LOCUS       DAOSMQ010000001        23596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_2171_length_23596_cov_4.828512, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000001 DAOSMQ010000000
VERSION     DAOSMQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 23596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23596
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_23596_cov_4.828512"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="PK829_00005"
     CDS             complement(<1..1789)
                     /locus_tag="PK829_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOU39631.1"
                     /translation="MATVIGGRWRPKAAFLPALRYLRQPVQLARGYQRADLRADLIAG
                     LTVGVVLLPQAIAFSLLAQLPPQTGLYTAVVGGIAAALWGSSEQLHSGPTNTISLLVF
                     STLAVSIIPGSDQYILAAGMLALMAGVLQLGLGLLRLGFIVNFVSHSVIVGFGTAAGV
                     LILVRQIDPLLGLSLPKTNILSGLVNSALAVSQVHLLTAAIGLGSVAIILLLRRLNPR
                     LPGALIALLAATAVVALLGDRAEGVAVIGAIGGGLPRPVSLPVLNLQLISDLSTGALA
                     VAAIGLVQTTAVSRSLASQTHQRLDNNQEFVGQGIANVLSGLFGGYATSGSFTISAVK
                     YRAGARTRLASLAAALFVLLVMLVAGPVGEYMPVSAIAAALIVTAYNMLDKAEIRRIL
                     RGAPGDAVIMVVTFLGTLFLNLDFAVLLGILLSFVLYVMRTSAPRVQVVVPGADFRHF
                     LYRPDAPSCPQLGIVEIQGDLYFGAVNHIEEAILHNAAQYPEQRFLIIRMHHVNQIDF
                     SGIHMLESLVQTYRDRGGDLFLVRVAPQARRLMQTTGCLGYLGEDNILDEDDAIEYVF
                     RHILDPAVCIYECPVRVFRECQNLPKRFDL"
     gene            complement(1793..2419)
                     /locus_tag="PK829_00010"
     CDS             complement(1793..2419)
                     /locus_tag="PK829_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU39632.1"
                     /translation="MKILAVSDRVLDKLYTGQTRQTYADVDLLIGCGDLPFYYLDFLT
                     SAIDAPLVYVRGNHDEGPQLAADGSVWRGVRGGVDIHGKVVTRKGLILAGLQGSMRYK
                     AHADYMYSETEMRRVVAQLVPRLLWNRQRLGRALDILVTHSPAYGIHDRPDLPHTGFK
                     VFLTFLRLFRPRYHLHGHIHIYRQDEVRETHFEDTTSINVYPYRVLEI"
     gene            complement(2416..2721)
                     /locus_tag="PK829_00015"
     CDS             complement(2416..2721)
                     /locus_tag="PK829_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOU39633.1"
                     /translation="MELKDLEARMHAFVAAKGWYANDSSKPQTAQNLAISLALEAAEV
                     LEHFQWRPEARDRDALAGELADVALYLLQLASVCGIDLETAILDTLRRNQEREWPDP"
     gene            complement(2881..3900)
                     /gene="gap"
                     /locus_tag="PK829_00020"
     CDS             complement(2881..3900)
                     /gene="gap"
                     /locus_tag="PK829_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU39634.1"
                     /translation="MTVKVGINGFGRIGRQVYKAIRDNYKGVLDVEAINDLMPPETNA
                     HLLKYDSTYGKFNGTVEVRDGDIYVDGEKLKSYAEKDPSKLPWGELGVDIVLECTGRF
                     RGKATAGAHLTGGAKKVIISAPGDKDIDGTFVLGVNEDKYDPATQHVISNASCTTNAL
                     APVAKVLHENFGIRRALMTTIHAMTNDQSILDVAHKDLRRARTTASNIIPTSTGAAKA
                     VGLVLPDLKGKFNGMAFRVPTVTVSVVDLTAELERPATKEEINAALKAASEAPGWLGK
                     VLNYTEEPLVSSDLIGDPASTTVDSLSTDVLDGNFAKVVSWYDNEWGYASRLADLTAY
                     VAERL"
     gene            4091..5320
                     /gene="hemW"
                     /locus_tag="PK829_00025"
     CDS             4091..5320
                     /gene="hemW"
                     /locus_tag="PK829_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOU39635.1"
                     /translation="MIAYALYLHIPFCQQRCSYCDFNTYTTLGGLQAAYVAALAMEIR
                     QVGALAAAAGDPRPAVRTIFLGGGTPSLLSPEQLGDILAAARAAFAIQPDAEITMEAN
                     PGTVSAGTLAAYRALGVNRLSFGVQSALPGELALLGRAHDFATAVEAVGLARAAGFDN
                     LNLDLIYGLPGQSVADWSQTLAAVLGLAAAEPAITHISLYCLTIEPGTPMQRWLSGGA
                     VPTPDADVAADQYELAGRELAAAGFDHYEISNWARPGRECRHNVVYWRNEPYLGLGAG
                     AHGLAGGYRYQVVRQPRAYVRRMLGAVWGSGGAEERGSRGEAAQFPLSSAVAAYHQVS
                     PEEAMSDTAITQLRLLDEGLDMAGFTARFGRAFDDVYANEVRQLEAWKLLHRRHGRLL
                     LTERGRFLSNQVFYRFV"
     gene            5377..5955
                     /locus_tag="PK829_00030"
     CDS             5377..5955
                     /locus_tag="PK829_00030"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOU39636.1"
                     /translation="MTKLTIAIQAGGKSSRMGTDKSFVPFLGRPMIEVVRERVEGLGD
                     ELILITNTPEPYAYLGLPMFGDDYPDTGPLGGIYTAIRHARNPHVLIVACDMPWLNRS
                     LLEYQVSLRETADIVVPRWDKFPEPLHAVYSQACLEPIREKLAAGMLKITAFFGRVSV
                     RFVDRPEIERFDPQGRSFINVNTPDDLAEQQR"
     gene            complement(6066..>6914)
                     /locus_tag="PK829_00035"
     CDS             complement(6066..>6914)
                     /locus_tag="PK829_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39637.1"
                     /translation="GGEGTRMRGGTLTKKELVEIGGRPIIWHVMRIFSAHGFNRFVLP
                     LGYEAQQLRRYFLDYEAMTRDFTIRLGSPTRSEGKDPLPNPLPGGEGTGAEGPLPGGE
                     GTGKIAFRGPEQHPEWQVSLVDTGLRTDKAGRIGRVADYLTTDRLFVTYGDGVGDVDV
                     TALLAFHRAHGKLVTITAIQPKHYQYGIMDAAEDGFVRQYVQYPELPYWINAGFMVFE
                     RATLDWMRGGDAQALETGVLQELIAAGEVMMYRHGGFWQSMDTLKDANDLERLWQQGA
                     PWRIWE"
     assembly_gap    6915..7014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7301..8365)
                     /gene="mtnA"
                     /locus_tag="PK829_00040"
     CDS             complement(7301..8365)
                     /gene="mtnA"
                     /locus_tag="PK829_00040"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOU39638.1"
                     /translation="MRTVFWDYDTDHLRMIDQRRLPFETVVPAYDTYQAVAEAIRDMV
                     VRGAPAIGAAAAFAMALAARQSRAADRLTLLRDLEAAAKALEAARPTAVNLAWAVRRQ
                     LRVAADEELESAGDVRHALLAEAQRLADEDVALNRRMGFYGAELIADGDTILHHCNTG
                     ALATVDWGTALGVVFAAHEQGKRVHVLVDETRPRLQGARLTAWELQQRGIPFDLIADN
                     AAGHFMRRGEVNLVLVGSDRTAANGDVANKIGTYQLGVLARENGVPFYPVVPTSTIDL
                     DLATGDLIPIEERSMDEVLTVIDTAIAPAGITARNPAFDVTPHRYVTGIITEAGIVYP
                     PFEKNLRAAVARAREIKWDE"
     gene            8565..10037
                     /gene="yvcK"
                     /locus_tag="PK829_00045"
     CDS             8565..10037
                     /gene="yvcK"
                     /locus_tag="PK829_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOU39639.1"
                     /translation="MSRLRALWQALYVRRRWLAVGIGVKRWLVLLMAGAAVTSIGWVY
                     LLLALNREGWLPPALFDVTTLRFLPLWLRALLPLLLGGAIMLLAVFQLGRSLTDPLRG
                     GEGAVVDSLYDYRRRDRGPHIVAIGGGTGLPGLLRGLTAYTSNITAIVTVADDGGSSG
                     RLRREMGLLPPGDFRNNLAALARDEALMTQVLQYRFGEQVPGARGQGPGEEAHARPGE
                     EAHAKDAKEEKEAASSPPLRPGPFAPLRERSPLEPSSLSGHAFGNLLLAALTGITGSF
                     DEALLAAQRVLALRGRVLPSTLEDVTLVGEVVMPDGETQRVAGESAIPQAGGRVERVR
                     LEPAQARAYPPALRAIFSAELIVLGPGSLYTSILPNLLLSDLAEALVHARGRVVYVCN
                     LATQPGETDGYSVADHVAAMQRHLPPGCIDIVLANDNLAVPPETGGGHTCFVPPVPPA
                     GVAMMTADLVDETRPWRHDSEKLARAVMALLARGQEISEV"
     gene            10078..10626
                     /locus_tag="PK829_00050"
     CDS             10078..10626
                     /locus_tag="PK829_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39640.1"
                     /translation="MTQFRLAIDGQERAFEVSQQGEQLHVSASPAEGDGDAGLTAEVR
                     VLHRDGPRLLLEIAWPDGTTERVHVAGARQGDRRQLWVEGRGLTAERVRRRGGGSAAT
                     QGSLAAAIPAVVSQILIAPGDAVAAGDKLILLESMKMVIPIVAPRDGVVARVLCALGE
                     SVPAGVPLIELADAPAAGNNPG"
     gene            10652..11755
                     /locus_tag="PK829_00055"
     CDS             10652..11755
                     /locus_tag="PK829_00055"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOU39641.1"
                     /translation="MNDDRLSRLTEQIAAHGLDGVALMPGPNLLYVSGMHVHLSERPI
                     LLFVPADDAPAIIIPTLEAMKAEAAGIAADRIFAWDDTEGYAGAFQRACAHLELTDYL
                     LGVEALHMRVLEMQMLQRYAPGLQIAHAEPALSALRSVKDAAEIAAMEKAIAVAERAL
                     QRILPRIKVGLSERQVAAMLTQELLASGADSIAFGPIVAAGPNAALPHATPTDRKLQR
                     GDLLIIDWGVFVDDYPSDLTRTFAVGEIDPELRRIYNTVLLANEQARLATRPGVSGRD
                     VDRAARDVIDDAGYGEYFIHRTGHGLGLEVHEPPDASPANRQPLAAGNVFTVEPGIYL
                     PGRGGVRIEDNVAVIADGCRTLTSFPRELMTVG"
     gene            complement(11813..12865)
                     /gene="dinB"
                     /locus_tag="PK829_00060"
     CDS             complement(11813..12865)
                     /gene="dinB"
                     /locus_tag="PK829_00060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOU39642.1"
                     /translation="MSARAVLHIDMDAFYVNVHLLDHAGDSGVPLVVGGQPDERGVVT
                     SASYEARRLGVRSAMPTSQALRLAPGLRIVPPDWPRIRQCSREVMAVLGAFGPLEQMS
                     VDEAYVDAGGWEGAEAIARAIFERVPAETGLPCSVGLATSKLVAKVASDFNKPRGFTV
                     VPPGEEAAFLAPLPARVIWGIGPRTADRLAALGIATCGQLATADLERLRGVFGREAEK
                     LTRRAQGLDDRPVEPDRGPAKSISQEWTFSRDVADPAVLRERLRQMSAEVAAELAQRN
                     LLAHTIRVKFRTPDFTTFTRQRTVEVGVDDAETIYRLALALWTEHWPAGKPMRLLGVG
                     AAGLVEGESRQLGLGL"
     gene            complement(12882..14240)
                     /locus_tag="PK829_00065"
     CDS             complement(12882..14240)
                     /locus_tag="PK829_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HOU39643.1"
                     /translation="MDERTPTFTFWRALRLSSFQIGSAMGDILVTSIWNRILITNFGI
                     PATPVGLLIALRYLLSPLSLWAGNLTDSKRIFGLRRTPIIWLGRALMVLSLPLLGLSV
                     LRLGGSSSDVLGWLAALLSSVMYGTGTLISGSPFLALVRESAPEARQGVAISLVETIL
                     IVFFAIAGIGFSLWMPVYDVRIFWEMIAVTMLVGGFFWVFAIIGVERRRRPDVAALAT
                     SEPGRVPLGVVLREIWADQRTRRFFAFLSLATLAAWVQDAILEPFGGDVFHLDIGVTT
                     RFNSYWQTATVLTLVIGAILWRKRRPERQAPIAIAGLATMAIGLMLLAAAGALGQRHL
                     IELALFVFGGGFGIYTFGGFSLMAVMSPDRHAGAYLGLWSLSVVVFKGLGALIGGVLR
                     DLFLLSLGLSPGLSYGLVFLIQALGLVAALLILARVDVLGFARDTGRHVPLAEARAAA
                     AD"
     gene            complement(14283..15464)
                     /locus_tag="PK829_00070"
     CDS             complement(14283..15464)
                     /locus_tag="PK829_00070"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HOU39644.1"
                     /translation="MPDRRVQLADEFRSQARFAAGYSPLYALLFGIVAGWLAAEEPDP
                     VADWLLVATAERRPFDVTLLLPAALHREVLAGVPAAAPLAPFYPSVGGTVPAAPAADL
                     AAALRQVSLARGDALAAFIRRANVQTNETGRGLAWLLPLAVMGWPAVHLLELGASAGL
                     NLVAERRAYRLADAAAPETTLLTLGSGAPPQFTTLVRGWATLPPRPCCPTILSRTGVD
                     LHPFPLATAEDELTLTAFLWADQVTRVARLREGIAALRAVEATVAPVRLAPLRLPDEL
                     PAFLAGYAPPPDDAPVVIFNTTVTMYLPDRGAGLRAAIDDWAGRRAAPVLWLQWEPAG
                     PDDGPPPEDSWFRWTADLWPNAPGKYRRHHFQLAWVHPHGTALEWTPQIGSFLDIAMQ
                     I"
     gene            complement(15533..16681)
                     /locus_tag="PK829_00075"
     CDS             complement(15533..16681)
                     /locus_tag="PK829_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOU39645.1"
                     /translation="MREVAILGVGQTPVREHWDLSLRQLAVQAGRAALDDAGVERIDA
                     LYVSNMNSDRLAGQRHLGALVADYLGQRGVEAVKLEAACGSAGSAMRQGLLAVASGQL
                     DAVLVIGVEKLTELPGRQTTAALATAADADYEVEHGITFVGLNALIMRRYMHEFGYSH
                     RDFAPFAVTAHANAARNPNALYRYAIDASEYERGREVADPIRLYDASPIGDGAAALLL
                     VPAERAPGAIRVVGSAAATDTLAVHDREDMLRLRAAERSAQRAYRQAGVGPEDIDLFE
                     LHDAFSIMAVLSLEACGFAARGQGVRLGLDGDIVQGGRIPISTMGGLKARGHPVGATG
                     LYQLVEATLQLRGQAGPLQVAGARTAMAQNIGGSGATIVTHILQTPGD"
     gene            complement(16859..17944)
                     /locus_tag="PK829_00080"
     CDS             complement(16859..17944)
                     /locus_tag="PK829_00080"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOU39646.1"
                     /translation="MDIELIPLADAQTRVPPTEDFGFCVHFANRMFTQRYNHGRGWHE
                     ARIEPYGPLALSPATAVLHYAQLIFEGMKAYRRPDGDINLFRPMENMRRFNNSARRMA
                     MPTVDEEEHLEAICRLVELEHDWVPSAPDATLYIRPTMVATDDALGVRASANYLHFVI
                     ISPVGPYFREGFSPVPVYITDEYVRAVTGGVGEAKTGGNYAASLYAGEEARRRGYSQV
                     LWLDGREHRWVEEVGAMNICFVYDNHRIVTPSLTGSILPGVTRKSVIELGRALGYEVA
                     EEMIDVHEMLADVKSGRITEVFGCGTAAVIAPVGKFGFHDETVLINDNQAGPVARELY
                     QHLTDIQFGRVPDPFGWTYTIHANGHS"
     gene            complement(18002..18481)
                     /locus_tag="PK829_00085"
     CDS             complement(18002..18481)
                     /locus_tag="PK829_00085"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOU39647.1"
                     /translation="MTNANHRPRAAMAARKRIALVAHDNKKDDLLEWTSFNLDLLRHH
                     KFYATGTTGAILVDRLGLEVTRMQSGPLGGDQQLGAHIVDGDIDILIFFWDPLEAQPH
                     DPDIRALLRIAAVWNIPVATNRATADFLISSPHMTEEYERLLTDYYDYQHRLDKAGD"
     gene            18695..19540
                     /locus_tag="PK829_00090"
     CDS             18695..19540
                     /locus_tag="PK829_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOU39648.1"
                     /translation="MRFTLGLAQVAPVLGDLAANLQLHLDMAAAAAGRGVDLLIFPEL
                     ALTGYDLGEQAFDVAIRADATDPTFAALLAASQQLDLVVSFVEVDERHRYYITAVYLS
                     GSRLVHRHRKVYLPTYGDFDEAHTFAHGDRVRAFDTRFGRVGLLVCEDFWHASMPYLL
                     WQDGADILLLLSASLEHGQAEATSTADKVLAINRAYALLFTDFVVHVNRVGREGDGTF
                     WGGSTVYGPDAALLAEGPHGEPALVVATLDTDALRAARLRLPLLRDERPDLTARELER
                     MTHER"
     gene            19530..20486
                     /gene="era"
                     /locus_tag="PK829_00095"
     CDS             19530..20486
                     /gene="era"
                     /locus_tag="PK829_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOU39649.1"
                     /translation="MSAEQTTPTNEESAGYETPAGHRSGFVALVGRPNVGKSTLMNAF
                     MRQKIAAVSHRPQTTRTRQLGIITEPDYQIVFVDTPGIITQPRHELDNYMVQAALETL
                     DDADVILWLLDLTETPDEDDRLIAERLAGLKSSKVLLGLNKTDAVAAADILSRTEAYT
                     ALLPGAEWHHLSAATGAGVAELLARLAAALPEGPRYYPQEQVTDIFVRNIAAELIREQ
                     LMLQLRDEVPYGTAVQVRDFKERDNGTVFISADIFVERDSHKSIIIGAGGAQLKQLGA
                     AAREQIEQLVDGKVFLELWVKVEPNWRRDSQLLRQFGYGPKE"
     gene            20621..21706
                     /locus_tag="PK829_00100"
     CDS             20621..21706
                     /locus_tag="PK829_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOU39650.1"
                     /translation="MTRQNANGVSRRSFIKVATVTAVAAAATGGGAAILKHTISPADV
                     VISTGPAPVAITSGGVVDIYPTLAPIVPAGQVVNSAEDALGQLAASQAENMQLRAQLD
                     QALRDLEAARAGEVNVRSAHDALSLELDGARTKLGVLGGLVALYEQLDGADLGGIVES
                     GLGAVGEKIGELLGGVPALAAGLDGGQLALSEVEAHIPLLDSGRQWLGVQGEKMRGFY
                     ADIEGWLQRAVDRVGNFLEMLQDWFEGMRRWLPGGAGEKAAGVMGALTALLAETPSTI
                     AGLDTNIAQPLDVWLQRIDGEPALQRRFLRPIRDEVIARARTTADQANQVGTVYEQQL
                     VAPARAALGNRATLRQAIADYRAQNQI"
     gene            complement(21795..23573)
                     /locus_tag="PK829_00105"
     CDS             complement(21795..23573)
                     /locus_tag="PK829_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl cyclase"
                     /protein_id="HOU39651.1"
                     /translation="MVLLLTLALGSSVSAAPPTPAATPDFGPNVIIFDPGMPTADIQA
                     TVDSIYAQQVDDEMGPNRYALLFKPGVYGSAATPLIVKVGYYTEVAGLGLLPTDVVIN
                     GHVDVYNRCLAADNCIALVNFWRSVSNLTINVMGLSDCRASGNFWAVSQASPMRRVNI
                     TGGNLTLMDYCTAGPQYASGGFIADSQTGYIINGSQQQFLVRNSIIGGWSNGVWNVVF
                     SGVLGAPPNSFGSTPYDPPPYTTLDVSPLTREKPFLYVDDNGKFNVFVPTARMRSAGT
                     TWSDGPAAGISIPINKFFLARPTDSISKINLALKRGMNVIFTPGVYQIDKTLFVNRRD
                     TVVLGLGIATLVPQHGNMAMRVANTPGVILAGLMFDAGPQNSSALLQIGSRHVPVRPN
                     DHRNPTLLADIFFRIGGATPGKATTSLVVNSNYVVLDNIWAWRADHGNGVGWTENTAD
                     TGVIVNGNFVTAYGLFVEHYQKTETIWNGEYGRTIFFQNEMPYDPPSQAAWQHDGVNG
                     YPAYKVADSVTNHGAWGMGSYSFFNAGPDIHAERAFEAPVAPSVKFYSLVTVFLNGNG
                     GIDHVINDTGAAVNSTNQVSNVVSYP"
BASE COUNT         4119 a         7584 c         7957 g         3836 t
ORIGIN      
        1 tcagatcgaa gcgcttgggc aggttctggc actcacggaa aacgcgcacg gggcactcgt
       61 agatgcacac agccgggtca aggatgtggc ggaagacgta ctcaatcgcg tcatcctcgt
      121 cgaggatgtt gtcctcgccc agatagccca ggcagccggt ggtctgcatc aagcgccgcg
      181 cctgcggggc aacgcgcacg aggaacagat cgccgccgcg atcgcgatag gtctgcacca
      241 ggctctccag catatggatg ccgctgaagt cgatctggtt gacgtggtgc atgcggatga
      301 tcaggaaacg ctgttcgggg tactgggcgg cgttgtggag aatggcttcc tcaatgtggt
      361 tgaccgcgcc gaagtagagg tcaccctgaa tctcgacgat gcccagctgg gggcaactgg
      421 gagcgtcggg ccggtatagg aagtggcgga agtcagcgcc gggcacgacg acctgcacac
      481 gcggcgcgct ggtgcgcatg acgtagagta cgaaggacag caagatgccc agcaagacag
      541 cgaagtcgag attgaggaag agcgtgccca gaaaggtgac gaccatgatg acggcatcgc
      601 cgggcgcgcc gcgcaggata cgacgaattt cggccttgtc gagcatgttg taggccgtga
      661 cgatgagcgc ggcggcgatg gcgctgacgg gcatgtattc gcccaccgga ccggcgacga
      721 gcatgaccag caacacgaac agcgccgcgg cgagcgaggc cagccgcgtc cgcgccccgg
      781 cgcgatactt gaccgctgag atggtgaacg accccgacgt ggcgtagccg ccgaacagac
      841 cggacaggac gttggcgatg ccctggccga cgaactcctg attgttgtcc agccgttggt
      901 gggtctgcga ggccagcgag cgggacacgg ccgtggtctg caccagaccg atggcggcca
      961 cggccagcgc gccagtggag aggtcgctga tgagctgcaa gttgagcact ggcagcgaca
     1021 ccggtcgcgg cagcccgccg ccgatcgcgc cgatgacggc cacgccctcg gcgcggtcgc
     1081 ccagcagcgc cacgacggcc gtggccgcca gcagggcgat gagcgcgcca ggcaggcgcg
     1141 ggttgaggcg gcgcagcaac aaaatgatgg ccactgatcc cagaccgatg gccgccgtca
     1201 gcaagtgcac ctgggacacg gccagagcgg aattgacaag gccactgagg atgttggtct
     1261 tgggcagcga cagacccagg agcgggtcga tctggcgcac aaggatgagc acaccggcgg
     1321 cggtaccgaa gcccacgatg acggaatggg acacaaagtt gacaatgaac cccagccgca
     1381 gcaagcccaa ccccaactgc aagacgccgg ccatgagggc caacatgccg gcggcgagga
     1441 tgtactggtc agagccgggg ataatcgaga cggccagggt ggagaagacg agcagggaga
     1501 tggtattcgt cgggccgctg tggagttgtt ccgacgaacc ccacaacgcc gcggcgatgc
     1561 cgccgacgac ggcggtatag aggcccgtct gcggcggcag ttgggccagc agcgagaagg
     1621 cgatggcctg cggcaagagg acgacgccaa cagttagccc ggcgatgagg tcggcccgca
     1681 ggtcggcccg ctggtagccc cgcgccagct gcaccggctg ccgcaggtag cgcagcgccg
     1741 gtagaaaggc cgccttgggg cgccatcgcc cgcctatcac tgtggccatg ggctaaatct
     1801 caaggactcg atacgggtag acgttgatgc ttgttgtatc ctcaaagtgc gtctcgcgca
     1861 cctcatcttg ccgatagatg tggatgtggc cgtgcagatg atagcgcggg cgaaagagcc
     1921 gcagaaaggt caggaagacc ttgaagccgg tgtggggcag gtcgggccgg tcgtggatgc
     1981 cgtaggcggg cgagtgggtc accaggatgt cgagcgcccg gccgaggcgt tgccggttcc
     2041 agagcagccg cggcacgagt tgggcgacga cgcgccgcat ctcggtctcg ctgtacatgt
     2101 agtcggcatg agccttatag cgcatggagc cttgcagccc ggccaggatc aaccccttgc
     2161 gggtgacgac cttgccgtgt atatcaacgc caccgcgcac gccgcgccag acagagccgt
     2221 cggcggccag ttgcggccct tcgtcgtggt taccgcgcac gtagaccagc ggcgcgtcga
     2281 tggccgacgt cagaaagtcc aggtaataga acggcaagtc gccgcaaccg atgagcaagt
     2341 ccacgtcggc ataggtctgt cgggtttgcc cggtatagag cttgtccagc acgcgatcgc
     2401 tgacagccag gatcttcatg ggtctggcca ctcgcgctct tgattgcgcc gcagcgtgtc
     2461 gaggatggcc gtttccaggt cgatgccgca gacgctggcc agttgcagca aatagagggc
     2521 cacgtcggcc agttcgccgg ccagcgcgtc gcggtcgcgg gcttccggcc gccactggaa
     2581 atgctccaac acctcggccg cctccaacgc cagcgagatg gccaggtttt gcgccgtttg
     2641 tggcttagac gaatcgttcg cataccaacc cttggcagcg acaaaggcat gcatgcgggc
     2701 ttccaggtcc ttcagttcca taggtaagcg tcgtggcgcg tgggcgtagc aggccgacgc
     2761 gcggcaatgc cgacagagcc tattctagag aatcgggcgc ggggccgcaa aagaaaaccg
     2821 ccggcccaga ggtggaccgg cggtagttct atttcaccga acgttcggcc agaggtcggc
     2881 ttagaggcgc tcggccacgt aagcggtcag gtcggccaga cgcgaggcat agccccactc
     2941 gttgtcatac cagctgacga ccttggcgaa gttgccgtcg agcacgtcag tggacaggga
     3001 atcgactgtg gtcgaggccg ggtcgccgat gaggtcagac gacaccagcg gctcctccgt
     3061 gtaattgagc accttgccca gccagccggg ggcctcggag gcggccttca gggcggcgtt
     3121 gatctcttcc ttggtggccg ggcgctctag ctcggcggtc aggtctacga ccgacacggt
     3181 gacggtcggg acgcggaagg ccatgccgtt gaacttgccc ttcaggtcag gcagcaccag
     3241 gccgacggcc ttggccgcgc cggtggaggt gggaatgatg ttcgaggcag tcgtacgcgc
     3301 gcggcgaagg tccttgtggg ccacgtccag gatgctctgg tcgttggtca tggcgtggat
     3361 ggtggtcatg agagcgcggc ggatgccgaa gttctcatgc agcaccttgg ccacgggtgc
     3421 cagggcgttg gtggtgcagc tggcgttgga gatgacgtgc tgcgtggccg ggtcgtactt
     3481 gtcttcgttg acgccgagga cgaacgtgcc gtcgatgtcc ttgtcaccgg gcgcggagat
     3541 gatgaccttc ttggccccgc cggtcaggtg ggcgccggcg gtggccttgc cacggaagcg
     3601 gccggtgcac tccaggacga tatcgacacc cagctcgccc cagggcagct tggaggggtc
     3661 cttttcagcg tagctcttca acttctcgcc atcgacgtag atatcgccgt cgcggacttc
     3721 gaccgtgccg ttgaacttgc cgtaggtgga gtcgtacttc agcaggtggg cgttggtctc
     3781 cggcggcatc aggtcgttga tcgcttcaac gtcgagaacg cccttgtaat tgtcacggat
     3841 cgccttatag acctgacggc cgatacggcc gaatccgttg attccaacct taacagtcat
     3901 ggaaacactc cttatgtgtg gtgatgtggg taggaaggtg cagattgtgc gagatggtac
     3961 agtgcgcagg aagcagtggg aagcgcactg tcacccatta taccgaatag agggtctagg
     4021 caaaagggat aatcacaaaa tccacagtct tctacactgc cgcgccaaaa tcgggcataa
     4081 tcccgcctgt atgatcgcct acgcgctcta cctgcacatc ccgttctgcc aacaacggtg
     4141 tagctattgc gacttcaaca cctacaccac cctcggtggc ttgcaggcgg cctacgtcgc
     4201 cgcgctggcg atggagattc gccaggtggg cgcgctggcc gcggccgcgg gcgacccgcg
     4261 cccggccgtg cgcaccatct tcctcggcgg cggcacgcca tcgctgctat cgccggagca
     4321 gctgggcgat attctggccg ccgcccgcgc cgccttcgcc atccagcctg acgcggagat
     4381 cacgatggaa gccaaccccg gcacggtctc ggccggcacg ctggcagcct atcgcgccct
     4441 gggcgtcaac cggctcagct tcggcgtgca gagcgcgctg cctggcgagt tggccctact
     4501 ggggcgcgct cacgactttg ccaccgccgt cgaggccgtc ggcctggccc gcgccgccgg
     4561 cttcgataac ctcaacctcg accttatcta tggcctgccg gggcagagcg tggccgactg
     4621 gtcgcagacg ctggcggccg tgctcggcct ggccgcggcc gagccggcca tcacccacat
     4681 cagcctctac tgcctgacca tcgagccggg cacgccgatg cagcgctggc tgtcgggcgg
     4741 cgccgtcccc acgcccgacg cggatgtggc cgccgaccag tacgaactgg ccgggcggga
     4801 actggccgca gcggggttcg accactacga gatctccaac tgggcgcgac cagggcggga
     4861 gtgccgccac aacgtcgtct actggcgcaa cgagccgtac ctggggctgg gcgcgggcgc
     4921 gcatggcctg gccggcggct atcgctatca ggtcgtccgc cagccgcggg cgtatgtgcg
     4981 gcgcatgttg ggtgcggttt ggggaagcgg gggagcagag gagcggggga gcagagggga
     5041 agcggcgcag tttcccctgt cgtcggcggt ggccgcctat caccaggtca gcccggaaga
     5101 ggctatgtcg gacacagcca ttacccaact gcgcctgctg gacgaaggat tggatatggc
     5161 cgggttcacc gcccgcttcg gccgtgcctt tgatgatgtc tatgcgaatg aagtccggca
     5221 actggaggcc tggaagctcc ttcatcgccg ccacggccgc ctgcttctga cggagcgcgg
     5281 gcggtttctc agtaaccagg tattctatcg gttcgtctag gctgaccgcc gacgacagac
     5341 ggccagccgc cgaccactca ccaaccgact actgctatga ccaaactaac aatcgccatc
     5401 caggctggcg gcaagagcag ccgcatgggc acggacaagt catttgtgcc ctttctgggg
     5461 cggccgatga tcgaggttgt ccgcgagcgc gtcgaggggt tgggcgatga gctaatcctc
     5521 atcaccaaca cgccggagcc gtatgcctac ctgggcctgc ccatgttcgg cgacgactac
     5581 cccgacacgg ggccgctggg cggcatctac acggccatcc gccacgcccg caatccccat
     5641 gtgctcatcg tcgcctgcga catgccctgg ctgaaccggt cgctgctgga atatcaggtc
     5701 agcctgcgcg agacggccga catcgtcgtg ccccgttggg acaagttccc ggagccgcta
     5761 cacgctgtct atagccaggc ctgcctggag ccgatccgcg agaagctggc ggcgggaatg
     5821 ttgaagatca cggccttctt tggccgcgtg tcagtgcgct ttgtggatcg gccggagatc
     5881 gagcgcttcg atccacaggg gcggtcgttc atcaacgtca acacgcccga cgacctggcc
     5941 gaacaacaga gataaagaag ctcacgcaaa ggacgctaag ggggcaaagg cgcaaagaag
     6001 acgctttctt cctttgcgac tttgcttact tggcgtcttt gcgtgagctt ctttgcgtga
     6061 gcttcttact cccagatgcg ccacggcgcg ccctgctgcc acaggcgctc cagatcattg
     6121 gcgtccttga gcgtatccat cgactgccag aagccgccgt ggcggtacat catcacctcg
     6181 ccagcggcga tgagttcctg caaaacgccc gtctccagcg cctgcgcgtc gccgccgcgc
     6241 atccagtcca gcgtcgcccg ctcgaagacc atgaagccgg cgttgatcca gtaaggcagt
     6301 tccgggtact ggacgtattg gcgcacgaac ccgtcctcgg ccgcgtccat gatgccatac
     6361 tggtagtgtt tcggctggat ggcggtaatg gtcaccagct tgccgtgggc gcggtggaag
     6421 gccagcagcg cggtcacgtc tacgtcgccg acgccgtcgc cgtaagtgac gaagaggcgg
     6481 tcggtggtga gatagtcggc cacgcggccg atgcggccgg ccttgtccgt gcgcaggccc
     6541 gtgtccacca gcgacacctg ccactccggg tgctgctccg gcccacgaaa ggcgatcttc
     6601 ccagttccct ctccccccgg gagagggcct tccgcccccg ttccctctcc ccccgggaga
     6661 gggttaggga gagggtcttt cccctccgag cgcgtgggcg agccaagacg aatggtgaag
     6721 tcgcgggtca tggcctcgta gtcgagaaag taacgccgca actgctgggc ctcgtagccc
     6781 agcggcagca caaagcggtt gaagccatgg gcggagaaga tacgcatgac gtgccagatg
     6841 atgggccggc cgccgatctc gacgagttcc ttcttggtca gcgtgccgcc gcgcatgcgc
     6901 gtgccctcgc cgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgacga
     7021 gttccttctt ggtcagcgtg ccgccgcgca tgcgcgtgcc ctcgccgcca cagaagatga
     7081 ctaccggaat gtcttcgttg ctcatgttca agagagatta tccgtacatt gggaagactc
     7141 acgccaaggc gcgaaggagc caaggcgcaa agaagttttt ctctatcctc tttgtctttg
     7201 cttctctgcg tctctgcgtc tctgcgtctt tgcgtgagcc tttcttctct tccttattct
     7261 tctttgcgcc tttgcccctt agcgccttgg cgtgagcgtt ttactcgtcc cacttgatct
     7321 cccgcgcccg ggcgacagcg gcgcggaggt tcttctcgaa cggtgggtag acgatacccg
     7381 cctcagtgat gatgccagtg acgtagcgat ggggcgtcac gtcgaaagcc gggttgcggg
     7441 cggtgatgcc cgccggggcg atggcggtgt cgataacggt caacacctcg tccatgctgc
     7501 gctcttcgat ggggatcagg tcgcccgtgg ccaggtcaag gtcaatggtg ctggtgggca
     7561 cgacggggta gaacggcacg ccgttctcgc gggccagcac gccgagctgg taggtgccga
     7621 tcttgttggc cacgtcgccg ttggcagcgg tgcggtcaga gccgacgagg acgaggttca
     7681 cttcgccgcg gcgcatgaag tggccagcgg cgttatcggc gatgaggtcg aaggggatgc
     7741 cgcgctgctg gagctcccag gcggtcaggc gcgcgccttg caatcgcggc cgggtctcat
     7801 cgaccagcac gtggacgcgc ttgccctgct cgtgggcagc gaagacgacg cccagggccg
     7861 tgccccagtc gacggtggcc agcgcgccgg tgttgcagtg gtggaggatg gtgtcgccgt
     7921 cggcgatgag ttccgcgccg tagaaaccca tacgccggtt gagggccacg tcctcgtcgg
     7981 ccaggcgttg ggcctcagcc agcagggcgt ggcgcacgtc gccggccgac tccagctcct
     8041 cgtcggcggc aacgcgcagt tgccggcgga cggcccaggc caggttgacg gccgtcggcc
     8101 gggctgcctc cagggccttc gcggcggctt ccaggtcacg cagcagggtc aggcggtcag
     8161 cagcgcgact ctgccgggcg gccagggcca tggcgaaggc cgcggccgcg ccgatggccg
     8221 gcgcgccgcg cacgaccatg tcgcggatgg cctcggccac ggcctgatag gtgtcgtacg
     8281 ccggcacaac cgtctcgaac ggtagccggc gctggtcgat catccgcagg tggtcggtgt
     8341 cgtagtccca gaaaacagtc cgcatgcaat ctccttcctt tggaaccaat tggaaccacg
     8401 ctattctagt cgcttgtcgc ctcttgccaa acgacggcag tgatccgtga gccgtgacca
     8461 gtggccagtt cgcagagggc cggccactgg ccaccggcta ctgaccacca gccaccaccc
     8521 atccacgttc tatcctcccc caaaggagtt gctataatga ccccatgtcc cgcctgcgcg
     8581 ctttgtggca agccctctac gtccgtcgcc gctggctggc ggtcggcatc ggcgtcaagc
     8641 gctggctggt gctactgatg gccggcgcgg ccgtcaccag tatcggctgg gtttacctgt
     8701 tgctggccct caaccgggag ggctggctgc cgccggcgct gttcgacgtg acgaccctgc
     8761 gctttctgcc gctgtggctg cgagcgctgc tgccgctgct gctcggcggg gcgattatgc
     8821 tgctggctgt cttccagctg ggtcgcagcc tgaccgatcc gctgcgcggc ggcgaagggg
     8881 ccgtcgtcga ttccctttac gattaccggc gccgcgatcg cggcccccac atcgtcgcca
     8941 tcggcggcgg cacgggccta ccgggtctgt tgcgcggcct cactgcctac accagcaaca
     9001 tcaccgccat cgtcactgtg gccgacgatg gcggcagcag cggccgtctg cgccgcgaga
     9061 tggggctgtt gccgccgggc gacttccgca ataacctggc cgccctggct cgcgacgagg
     9121 cactgatgac gcaggtgctc cagtatcgct ttggggagca ggttccgggg gccaggggcc
     9181 aggggccagg ggaagaagct cacgcaaggc caggggaaga agctcacgca aaggacgcaa
     9241 aggaagaaaa ggaagctgct tcttctcctc ctttgcgccc tggccccttt gcgcctttgc
     9301 gtgagcgttc tccactggaa ccttcttccc tcagcggtca cgccttcggc aacctgctgc
     9361 tggcggcgct gacgggcatc actggcagct tcgacgaggc actgctggcg gcgcaacgcg
     9421 ttctggccct gcgcggccgg gtgctgccct cgacgctgga ggatgtcact ttggtgggcg
     9481 aggtggttat gcccgacggc gagacgcaac gcgtggccgg cgagtcagcc atcccccagg
     9541 caggcggccg ggtcgagcgg gtgcggctgg agccagccca ggcgcgcgcc tacccgccgg
     9601 cgttgcgagc catcttcagc gcggaactga tcgtcctggg gccgggcagc ctctatacca
     9661 gcatcctgcc taacctgctg ctgagtgacc tggccgaggc gcttgtccac gcccgcggcc
     9721 gggttgtcta cgtctgtaac ctggccacgc agccgggcga gaccgacggc tacagcgtcg
     9781 ccgaccacgt cgccgccatg cagcgccacc tgccgccggg ctgcatcgac atagtgctgg
     9841 ccaacgacaa tctggccgtg ccgccggaga cgggcggcgg ccacacgtgc tttgtgccgc
     9901 ccgtgccgcc ggccggcgtg gcgatgatga cggccgacct ggtcgatgag acccgcccgt
     9961 ggcggcacga cagcgaaaag ctggcgcggg cggtgatggc gctattagcg cggggacagg
    10021 agatatctga agtctgactc tcctgacaac cgcctatacc tctgctataa ctaacccatg
    10081 acccaatttc gtctcgccat cgacgggcaa gagcgtgcct ttgaagtgag ccagcaaggg
    10141 gagcaactcc acgtcagcgc cagcccggcc gaaggagacg gcgacgccgg cctgacagcc
    10201 gaggtgcgcg tgctgcaccg cgacgggccg cggctgctgt tggaaatcgc ctggcccgat
    10261 ggcacaactg agcgcgtcca tgtggccggc gcgcggcaag gggacaggcg gcagctctgg
    10321 gtcgaggggc gcgggctgac ggccgaacgc gtccggcggc gcggcggcgg cagcgcggca
    10381 acgcaaggct cgctggccgc ggccattccc gctgtcgtct cgcagattct gatcgccccc
    10441 ggcgacgccg tggccgccgg cgacaagctc atcctgttgg agtcgatgaa aatggtcatc
    10501 cccattgtcg cgccgcgcga cggcgtcgtg gcccgcgtgc tgtgcgcgtt gggcgaatcg
    10561 gtgccggccg gcgtgccgct gatcgaactg gccgacgcgc ctgcggccgg caacaacccc
    10621 ggctaactgc caagacaaaa ggaaagcgcc catgaacgat gatcgcctca gccgcctgac
    10681 cgaacagatt gccgcccacg gcctggacgg tgtcgccctg atgccggggc cgaacctgct
    10741 ctacgtcagc gggatgcacg tccacctcag cgagcggccc atcctgctgt ttgtccccgc
    10801 cgacgatgcg ccggcgatca tcatccccac gttggaggcg atgaaggccg aggcggccgg
    10861 catcgccgcc gaccgcatct tcgcctggga cgacaccgaa ggctacgccg gagccttcca
    10921 acgcgcctgc gcccacctgg aactgaccga ctacctgctg ggcgtcgagg cgctgcacat
    10981 gcgtgtgctg gagatgcaga tgctccagcg ctatgccccc ggcctacaga tcgcccatgc
    11041 cgagccggcg ctgtcggcgc tgcggtcggt caaggacgcg gctgagatcg ccgcgatgga
    11101 aaaggccatc gccgtggccg agcgggcgct gcaacgcatt ctgccacgca tcaaggtcgg
    11161 cctgagcgag cgacaggtgg cagccatgct aacgcaggag ctactggcct ccggcgcgga
    11221 cagcatcgcc ttcggcccca ttgtcgccgc cgggccaaat gccgctctgc cccacgccac
    11281 gccgaccgac cgcaagctcc agcgcggcga cctgctcatc atcgactggg gcgtcttcgt
    11341 cgatgactac ccctccgacc taacgcggac gttcgccgtg ggcgagatcg acccggagct
    11401 gcgccgcatc tacaacaccg tgctgctggc caacgagcag gcgcgtctgg cgacgcggcc
    11461 gggcgttagc ggacgcgacg tggaccgggc cgcccgcgat gtcatcgacg acgccggcta
    11521 cggtgaatac ttcatccacc gcaccggcca cgggttgggc ctggaagtac acgagccgcc
    11581 cgacgccagc ccggccaacc ggcagccgct ggccgccggc aacgtcttca ccgtcgagcc
    11641 gggcatctac ctgcccggcc gcggcggcgt gcgcatcgag gacaacgtgg ccgtcatcgc
    11701 cgacggctgc cggacgttga ccagcttccc gcgcgaattg atgacagtgg gctagacgag
    11761 ccggcggacg acgggaaagg cgcgccggct ggccagccgg cgcgcctgtt ttctacagcc
    11821 ccagccccaa ctgccggctc tcgccctcga ccaggccggc cgcgccaacg cccagcaggc
    11881 gcatcggctt gcccgcgggc cagtgttccg tccacagggc caaggccagc cggtagatag
    11941 tctcggcgtc gtccacgccc acctcgacgg tgcgctgccg ggtaaacgtg gtgaagtcgg
    12001 gcgtacgaaa cttgacgcgg atggtgtggg ccagcaagtt gcgctgggcc agctcggcgg
    12061 cgacctcggc cgacatctgc cgcagccgtt cgcgcagcac cgccgggtcg gccacatcgc
    12121 ggctgaacgt ccactcctgg ctgatgctct tggccggacc gcggtctggc tcgaccgggc
    12181 ggtcgtcgag gccctgggcg cgccgggtga gcttctccgc ctcgcggcca aagacgccgc
    12241 gcaaccgctc caggtcggcc gtcgccaact ggccgcaggt ggcgatgccc agcgccgcca
    12301 gccggtcggc tgtgcgtggg ccgatgcccc agatgacccg cgcgggcagc ggggccagga
    12361 aggcggcctc ctcccccggc ggcacgacgg tgaagccgcg cggcttgttg aagtcggagg
    12421 cgaccttggc caccagcttg ctcgtggcca ggccgacgga acagggcagg cccgtctcgg
    12481 ccggcacgcg ctcgaagatg gcccgcgcga tggcttcggc tccttcccag ccgccggcat
    12541 cgacgtaggc ctcatcgacg ctcatctgct ccagcgggcc aaacgcgccc aggacagcca
    12601 tgacctcgcg cgagcactgc cggatgcgcg gccagtcggg cgggacgatc ctgaggccgg
    12661 gggccaggcg aagcgcttgc gaagtgggca tggccgagcg gacgcccagc cggcgggctt
    12721 catagctggc cgaagtgacg acaccgcgct cgtccggctg gccgccgaca accaggggca
    12781 cgccgctgtc cccggcatga tcgagcagat ggacgttgac atagaaggcg tccatatcga
    12841 tatggaggac ggcgcgggcg gacatgcgcg gttgggccgg tttagtcggc cgccgcggcg
    12901 cgagcctcgg ccaggggaac gtggcggccg gtgtcgcgag cgaagccgag tacatcgacg
    12961 cgcgccaaga tgagcagcgc ggccacaagg cccaacgcct ggatcaagaa gaccaggcca
    13021 tagctgagac cgggcgacag gcccagcgac agcaggaaca ggtcgcgcaa gaccccgccg
    13081 atcagcgcgc ccagcccctt aaaaacgacg acggaaaggc tccacaggcc caggtaggcc
    13141 ccggcgtggc ggtcaggcga catgaccgcc atgaggctga agccgccaaa ggtgtagatg
    13201 ccgaaaccgc cgccgaagac gaacagggcc agttcgatga ggtggcgctg gcccagcgcg
    13261 ccggccgcgg ccagcaacat cagcccgatg gccattgtcg ccaggccggc gatggcaatg
    13321 ggggcctgac gctctggccg ccgcttgcgc cagaggatcg ccccaatgac aagagtcagc
    13381 acggtcgccg tctgccaata gctgttgaag cgtgtcgtca cgcctatgtc caggtggaag
    13441 acatcaccgc cgaacggctc caggatagca tcctgcaccc aggcggccag cgtggccagc
    13501 gacaggaagg cgaagaagcg acgcgtgcgc tggtcggccc agatctcgcg caggacaacg
    13561 cctaatggga cgcggccagg ctcagacgtg gccagcgcag cgacgtccgg ccgtcgccgc
    13621 cgctccacgc cgatgatggc aaacacccag aaaaagccac cgacgagcat ggtgacggca
    13681 atcatctccc agaagatgcg cacgtcatag acaggcatcc acaggctgaa accaatgccg
    13741 gcaatggcaa aaaagacgat gaggatcgtc tcaacaaggc taatggccac gccctggcgc
    13801 gcctccgggg ccgactcgcg cacgagggcc aggaaggggc tgccggagat gagcgtaccc
    13861 gtgccgtaca tgaccgaaga cagcagagcc gccagccaac caagaacgtc gctactcgac
    13921 ccacccaggc gcagaacgct gagacccaga agcggcagcg atagcaccat cagggcccgg
    13981 ccgagccaga tgatgggcgt gcgccgcagg ccgaagatgc gcttgctgtc ggtcaggttg
    14041 cccgcccaaa gactgagcgg cgacagcaga tagcgcaggg cgatgagcaa gcccactggg
    14101 gtggccggaa tgccgaagtt agtgatgaga atgcggttcc agatgctcgt caccaggatg
    14161 tcacccatgg ccgagccgat ctggaagctg ctgaggcgca gggcgcgcca gaaagtaaag
    14221 gtcggtgtgc gttcgtccat agcggcggga ttgtacgcga tggcgacaca atgcacaaac
    14281 gcttagattt gcatagctat gtcgagaaat gacccgattt gcggcgtcca ctccagggcc
    14341 gtgccgtgcg ggtgtaccca ggccagttga aagtggtggc ggcgatactt gccgggcgcg
    14401 ttgggccaca gatcggccgt ccagcgaaac cagctatcct ccggcggcgg cccgtcgtcc
    14461 ggcccggctg gctcccattg cagccacagc accggcgcgg cgcggcggcc cgcccagtca
    14521 tcgatagccg cgcgcaggcc cgcgccgcgg tcgggcaggt acatggtcac cgtcgtattg
    14581 aaaatgacca ccggcgcgtc gtccggcggg ggggcatagc cggccaggaa ggccggcaac
    14641 tcgtcgggca gacgcagcgg ggccaggcgc accggcgcga ccgtggcctc gacggcgcgc
    14701 agggcagcga taccctcgcg cagccgcgcg acgcgcgtca cctgatcggc ccacaggaag
    14761 gcggtcaacg tcagctcgtc ctcggccgtg gccaggggga aggggtgcag gtcaacgccg
    14821 gtgcgcgaga ggatcgtcgg gcagcagggc cgcggcggca gagtggccca gccgcgcacc
    14881 agcgtcgtga actgtggcgg cgcgccgctg cccagcgtca gcagcgtcgt ctctggcgcg
    14941 gcggcgtcgg ccaggcgata agcgcgccgc tcggccacca gattcagccc ggcgctggcc
    15001 cccagttcca acaggtggac ggccggccag cccattaccg ccagcggcag cagccaggcc
    15061 aggccgcgtc cggtctcgtt ggtctgcaca ttggcccggc ggatgaacgc ggccagggcg
    15121 tcgccgcggg cgaggctgac ctggcgcagg gcagcggcca ggtcagccgc cggcgcggcg
    15181 ggtacggttc cgccgacgct ggggtagaaa ggggccaacg gcgcggcggc gggcacgcca
    15241 gccaatactt cgcggtgcag cgccgcgggc agcagcaaag tcacgtcaaa cggccggcgc
    15301 tcggccgtag cgaccagtag ccagtccgca accgggtcag gctcctcggc cgccagccag
    15361 ccggcgacga tgccgaagag gagagcatag agcggcgaat aacccgcggc gaagcgcgcc
    15421 tgggagcgga actcatccgc cagttggacg cgccggtcag gcattcattg accccggtga
    15481 gctgccctgc aacccagccc ccgtcgtccg caggccattc tgcgcttctt cgtcaatcac
    15541 caggcgtttg caggatgtgg gtgacgatgg tcgcgccgct gccgccgatg ttctgggcca
    15601 tggccgtgcg cgcgccggcg acttgcagcg ggccggcctg gccccgtagc tgtaacgtcg
    15661 cctcgacgag ctggtagagg ccagtcgcgc cgacggggtg gccgcgggcc ttcaagccgc
    15721 ccatcgtcga gatagggatg cgcccgccct ggacgatgtc gccatccaaa cccaggcgca
    15781 cgccctgccc gcgcgcggca aagccgcagg cctccaacga cagcacggcc atgatgctaa
    15841 aggcgtcgtg caactcgaac aggtcgatgt cctccgggcc gacgcccgcc tgccggtagg
    15901 cgcgttgggc cgagcgctcc gcggccctca ggcggagcat gtcctcgcgg tcgtggacag
    15961 ccagcgtgtc ggtggccgcg gccgagccga cgacgcggat ggcccccggg gcgcgctcgg
    16021 ccggcaccag cagcagcgcc gctgcgccgt cgccgatggg cgaggcgtcg tagaggcgga
    16081 tgggatcggc cacctcgcgg ccgcgctcgt actcgcttgc gtcaatggcg tagcggtaga
    16141 gcgcgttggg gttgcgggcg gcgttggcgt gggccgtcac ggcgaagggc gcaaagtcgc
    16201 ggtggctata gccgaactcg tgcatgtagc ggcgcatgat caacgcgttc aggccgacaa
    16261 aagtgatgcc gtgctccacc tcgtagtcgg catcagcagc cgtggccagc gcggccgtcg
    16321 tctggcggcc gggcaactcg gtcagcttct ccacgccaat caccagcacc gcgtcgagct
    16381 ggccactggc gacggccagc aaaccctggc gcatggccga cccggccgaa ccgcaggcag
    16441 cttccagctt gaccgcctcc acgccgcgct ggcccaggta gtcggccacc aatgcgccca
    16501 ggtggcgctg cccggccagg cggtcgctgt tcatattgct gacgtagagc gcgtcaatgc
    16561 gctcgacgcc ggcgtcgtcc aaggccgcgc gcccggcttg cacagccagt tggcgcagtg
    16621 ataggtccca gtgttcgcgc accggcgtct gcccgacgcc gaggatggct acttctcgca
    16681 taggtctccg gaaatggtgg ctagtggatg atggccggtg gccagggatc cgcattcacg
    16741 attgggccgg tcgttgggac cggccctgct attaagtcta gcgcgggaaa ctaccagaaa
    16801 ctatcagggt ggctattggc ggggctggcc gtcatcaacg ccgcgccagt cctgtctgtt
    16861 acgagtggcc gttggcgtga atggtatacg tccagccgaa cgggtcgggg acgcggccaa
    16921 actggatgtc ggtcaggtgc tgataaagtt cccgcgccac cggaccggcc tgattgtcgt
    16981 taatgaggac ggtttcgtcg tggaaaccaa acttgccgac cggggcgatg accgcggctg
    17041 tgccacagcc gaagacctcg gtgatgcgac cgctcttgac gtcggccagc atctcgtgca
    17101 cgtcgatcat ctcctcggcc acttcgtagc ccagagcgcg gcccagttcg atgactgact
    17161 tgcgcgtcac gccgggcagg atgctgccgg tcagtgacgg cgtgacaatg cggtggttgt
    17221 cgtagacgaa gcagatgttc atcgcgccga cttcctcaac ccagcgatgc tcccggccgt
    17281 cgagccacag cacttgcgag tagccgcgcc ggcgagcttc ttcgcccgcg tagaggctgg
    17341 cggcgtagtt gccacccgtc ttggcctcgc ccacgccgcc ggtcacggcg cgcacatact
    17401 catcggtaat gtaaacgggc acggggctga agccctcccg aaagtagggg ccgacggggc
    17461 tgatgatgac gaagtggaga tagttggcgc tggcccggac gccgagagcg tcgtcagtgg
    17521 ccaccatcgt cggccggatg tagagcgtgg cgtcgggagc gctaggaacc cagtcgtgct
    17581 ccagctcgac gagccggcag atggcctcta ggtgctcctc ttcatctacg gtaggcatgg
    17641 ccatgcggcg ggccgagttg ttgaagcggc gcatattctc catcggccgg aagagattga
    17701 tgtcgccatc cggccggcga taggccttca tgccctcgaa gatcagttgg gcgtagtgga
    17761 gcaccgcggt ggccgggctg agcgccaacg gcccatacgg ctcgatgcgc gcctcgtgcc
    17821 agccgcgacc gtggttatag cgctgcgtga acatccggtt ggcaaagtgg acgcagaaac
    17881 cgaagtcttc ggttggtgga acgcgtgtct gcgcgtcggc caggggaatc agttcaatat
    17941 ccattgctac tcctatgcta ctcctattct gcgctcggtg agatgagggt caggagccgg
    18001 ctcagtcgcc ggccttgtcc agtcgatgct ggtagtcgta gtagtcggtc agcaggcgct
    18061 catactcctc ggtcatgtgt ggcgaggaga tgagaaaatc ggccgtggcc cggttggtgg
    18121 ccacggggat gttccagacg gcggcgatgc gcaacagggc gcggatgtcg gggtcgtgcg
    18181 gctgcgcctc caagggatcc cagaagaaga tcaggatgtc gatgtcgccg tccacaatgt
    18241 gggcacccag ttgctggtcg ccgcccagcg ggccactctg catgcgcgtg acctctaggc
    18301 ccagcctgtc caccaggatg gcgccggtgg tgccggtggc atagaacttg tggtgccgca
    18361 gcaggtcgag gttaaaggac gtccactcaa ggaggtcgtc cttcttgttg tcgtgggcaa
    18421 cgagggcgat gcgcttacgg gcagccatcg ctgcgcgcgg tcggtggttg gcattggtca
    18481 taggtcgctc ccaaatcgct gcgaaaaggc gcgcgggccg cgcatgatcg atattgtacg
    18541 ccgatcaacg ccaaccgcaa ggtaagcagg agcacgcctg tgtctctgcc ctctggggct
    18601 gcccggctag atgcacagcc aaatgggcca caaccagaac atttgtgcca tttttcgcgt
    18661 ggttcctaac gggtctatct gctacaatgg ccccatgaga ttcaccctcg gcctcgccca
    18721 ggtcgcgccc gtgctgggcg atctggccgc caacttgcag cttcatctcg atatggccgc
    18781 cgccgcggcc gggcgcggtg tcgatctact catcttcccc gaactggccc tgaccggcta
    18841 tgacctgggt gaacaggcct tcgacgtggc catccgcgcc gacgccaccg acccgacctt
    18901 tgccgccctg ctggcggcca gccagcagtt ggacctggtc gtcagcttcg tcgaggtcga
    18961 tgagcgccac cgctactaca tcacggccgt ttacctctcc ggcagccggc tcgtccaccg
    19021 ccatcgcaag gtctatctgc ccacctatgg cgacttcgac gaggcccata cctttgccca
    19081 cggcgaccgg gtgcgtgcct tcgacacgcg cttcggccgt gtcgggctgc tagtctgtga
    19141 ggacttctgg cacgccagta tgccctacct gttgtggcaa gatggtgccg acattctgct
    19201 gctgctgtcc gcctctctgg aacacggcca ggccgaggcc acgagcacag ccgacaaggt
    19261 gctggccatc aaccgcgcct atgccctgct cttcaccgat ttcgtcgtcc acgtcaatcg
    19321 cgtcggccgc gaaggggacg gcacgttctg gggcggctcg acggtctatg gcccggacgc
    19381 cgctttgctg gccgaggggc cgcacggcga gccggccctg gtggtggcca ccctggacac
    19441 cgacgccctg cgcgcggctc ggctgcgcct gccgctcttg cgcgacgagc ggcccgacct
    19501 gacagcgcgc gaattggagc gaatgacaca tgagcgctga acagacgacc ccgacgaacg
    19561 aagagagcgc cggctacgag acgccggccg gccaccgttc cggcttcgtg gccctcgtcg
    19621 gccggcccaa cgtgggcaag agcacgctga tgaacgcctt catgcggcag aagatcgccg
    19681 ccgttagcca ccggccgcag acaacgcgca cgcgccagtt gggcatcatc accgagccgg
    19741 actatcagat cgtcttcgtc gatacgcccg gcatcattac ccagccgcgc cacgaactgg
    19801 ataattacat ggtgcaggcg gcgctggaga cgctcgacga cgccgatgtc atcctctggt
    19861 tgctcgatct gaccgagacg cccgatgagg acgaccggct catcgccgag cggctggccg
    19921 ggctgaagag cagcaaggtg ctcctggggc tgaacaagac cgacgcggtg gcggcagccg
    19981 acatcctgag ccgcaccgag gcctacacgg ccctgctgcc gggggcggag tggcatcacc
    20041 tgtcggcggc gacgggcgcg ggcgtggccg aactgctggc ccggctggca gcggcgcttc
    20101 cggaagggcc gcgctattac ccccaagaac aagtgaccga tatcttcgtg cgcaacatcg
    20161 ccgccgaact catccgcgag cagctgatgc tgcaattgcg cgacgaggtt ccctatggca
    20221 cggccgtcca ggtgcgcgac ttcaaggagc gcgacaacgg caccgttttc atcagcgccg
    20281 atatcttcgt cgagcgcgac agccataaga gcattatcat cggcgcgggc ggagcgcagc
    20341 tcaagcaatt gggcgcggcc gcgcgcgaac agattgagca acttgtcgac ggcaaggtgt
    20401 ttctagaatt gtgggtcaag gtcgagccga actggcggcg cgattcgcaa ctcttgcgac
    20461 agttcggcta tgggccaaag gagtaggccg gcgcaacggt gggagccggt catgcgtaga
    20521 ggatgagaag tccgtaacag atatcgggtt cacgtcgtct gcacactgga cggcgcgtgg
    20581 ccgcggccac ggattttggg cgaggtaaca ggtacacaac atgacccgac aaaacgcgaa
    20641 cggcgtctcc cgacgcagtt tcatcaaggt ggcgacggtg acggccgtgg ccgcggccgc
    20701 caccggcggc ggggcagcca tccttaagca tacgatctca ccagccgacg tagtcatctc
    20761 caccggtccg gcccccgtgg cgattaccag cgggggtgtg gtggatattt atccgacact
    20821 cgcgccgatc gtgcccgccg ggcaagtggt gaacagcgcc gaagacgcat tggggcaact
    20881 ggccgcgtcg caggcggaga atatgcaatt gcgggcgcaa ctcgaccagg cgctacgcga
    20941 cctggaggcg gcccgggccg gtgaggtcaa cgtccgcagc gcccatgacg cgctgtcgct
    21001 agagttggac ggcgcgcgca cgaagctggg cgttctcggc gggttggtgg cgctctatga
    21061 gcagttggat ggcgctgacc tcggtggcat tgtcgagagc ggactgggcg cggtgggcga
    21121 aaagatcggc gaattgctgg ggggtgtgcc cgcgttggcc gccgggctag acggtggcca
    21181 actggctctg agcgaagtcg aagcccatat tcccctgctc gacagcggcc ggcaatggtt
    21241 gggcgtccag ggggaaaaga tgcgcggctt ctatgccgac attgagggct ggttgcagcg
    21301 cgccgtcgac cgcgtgggta atttcctgga gatgctccag gactggtttg aaggcatgcg
    21361 gcgctggcta ccgggcggcg cgggcgagaa agccgccggc gtcatgggcg cgctgaccgc
    21421 gctgctggcc gagacgccca gcaccatcgc cgggctggac acgaacatcg cccagccgct
    21481 ggacgtgtgg ctgcaacgca tcgacggcga gccggccctg cagcggcgct tcctgcggcc
    21541 catccgcgac gaggtcatcg cccgggcacg cacgacggcc gaccaggcca accaggtcgg
    21601 cacggtctac gagcagcaac tcgttgcccc ggcccgcgcg gccctcggca accgcgccac
    21661 gctgcgccag gccatcgccg actaccgcgc gcaaaaccag atttaattgc gaattaggaa
    21721 ttaggaacca agagtccctt gagtccgcgc ttccttacca ttcctaattc ctaattcgcc
    21781 attccacatt catcttacgg gtagctgaca acgtttgaca cctggttcgt cgaattgaca
    21841 gccgcgccgg tgtcgttgat cacgtggtcg atgccaccgt tgccgttgag gaaaacggtc
    21901 accaggctat agaacttcac gctgggcgcg acgggagcct cgaaggcgcg ctcggcgtgg
    21961 atgtccgggc cggcgttgaa gaagctgtaa ctgcccatgc cccacgcgcc gtggttcgtg
    22021 actgaatcgg ccaccttgta ggcgggatag ccattcacgc cgtcgtgttg ccaggcggcc
    22081 tggctcggcg ggtcataggg catttcgttc tggaagaaga tcgtgcggcc atattccccg
    22141 ttccagatcg tctccgtctt ctggtaatgc tccacgaata gcccataagc cgtcacgaag
    22201 ttgccgttga caatgacgcc ggtgtcggcc gtgttctccg tccagcctac gccattgccg
    22261 tggtcggcgc gccaggccca gatattgtcc aacaccacgt agttgctatt caccacgagg
    22321 ctggtcgtgg ccttgccggg cgtggctccg ccaatgcgga agaaaatgtc ggccagcagg
    22381 gtggggttcc tgtggtcgtt cggccggaca ggcacatggc gcgagccaat ctgaagcagg
    22441 gccgaagaat tctgcgggcc agcgtcaaac atcagtccgg ccagtatcac ccccggtgtg
    22501 ttggctaccc gcatggccat gttgccatgc tgcggtacca atgtggcgat gcccaggccc
    22561 agcaccacgg tgtcgcgccg gttgacaaaa agtgtcttat caatctgata gacgccagga
    22621 gtaaagatca cattcatgcc gcgcttgagg gcgaggttaa tcttactgat gctatcggtc
    22681 ggccgggcga ggaaaaactt gttgatgggg atagatatgc ccgccgccgg gccgtcgctc
    22741 cacgtcgtgc cggccgagcg catgcgcgcc gtgggcacga agacgttgaa tttgccgtta
    22801 tcatccacgt acaggaacgg cttttcgcgc gtgagtgggc tgacgtccag agtcgtgtag
    22861 gggggtgggt cataaggcgt ggagccgaag gagttcggcg gcgcgccgag cacgccggag
    22921 aagaccacat tccagacgcc gttcgaccag ccgccgatga tgctattgcg caccaggaac
    22981 tgctgttgcg agccgttgat aatgtagccg gtctgggaat cggcaatgaa gccgccgctg
    23041 gcgtactgcg ggccggcggt gcagtaatcc atcaacgtca ggttgccgcc ggtaatattg
    23101 acccggcgca tcggcgacgc ctgcgagaca gcccagaaat tgcccgaggc acggcagtcg
    23161 ctaagaccca tgacattgat cgtcaggttg cttaccgacc gccagaagtt gaccagcgcg
    23221 atacaattat cggcggccag gcagcgattg tagacatcaa cgtgcccgtt gatcaccacg
    23281 tcggtcggca acaggcccag gccggcgacc tcggtatagt agcccacctt gacgatcagc
    23341 ggcgtcgcgg cgctgccata gacgccgggc ttgaaaagca gggcatagcg gttcggcccc
    23401 atttcgtcgt cgacttgctg ggcatagatg gagtcaaccg tggcctggat gtcggccgtg
    23461 ggcatgccgg gatcgaagat gatcacattg gggccaaagt caggtgtggc cgccggcgtg
    23521 ggtggcgcgg cggacacgct gctgcccaac gccagggtga gcaacagcac cataagcaag
    23581 gcgacggcta acgact
//
LOCUS       DAOSMQ010000002        21285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_2478_length_21285_cov_5.081959, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000002 DAOSMQ010000000
VERSION     DAOSMQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 21285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21285
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2478_length_21285_cov_5.081959"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK829_00110"
     CDS             complement(<1..390)
                     /locus_tag="PK829_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39652.1"
                     /translation="MLNKLAAIIRKDTLLRFEGRSELLFFLILPLVFTFLIGGGMPAA
                     SGGDNRIFLPVADEDGTARSAALVAALAAGDTVRPEVMGRANAEALLDDEAVAAVLVI
                     PAGFGAALTADGLAAGQPADVVVLALPT"
     gene            complement(423..1691)
                     /locus_tag="PK829_00115"
     CDS             complement(423..1691)
                     /locus_tag="PK829_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU39653.1"
                     /translation="MNKVLTIMWKDARVLFGDWAAVALIIAGPLVLALGMGLVTGAFS
                     GGDAPAIGRIPVVLVDEDGGQLAAALAGLLTGNDLSDLLAVEALTDPDAARARVAAGD
                     ASAAVLIPAGFSDSFMPDMATGELPAPVAAVVVGDPGAPVGAAVVRSVVDAFAARADT
                     AVAGVRAGLTQLASSGAASPAELPALGQRLGEALFADGAVAATASVIRVETRNVTATG
                     QPFNPLAYFAPGMALLFLMYAVTLGARTLLSERREGTLARMLAAPVSSAQVLGGKVGG
                     IFLGGFIQLAALILLTTLLFRLNWGNPLSVALLVVAAALAATGWGLLIAAVAANSGQI
                     TNMGMALTLLFGILGGSFVPLNDAWPALDWLSRITPNRWALDGFTALAAGDGGAAVLV
                     PVAALLVMAVVLFAAAALLFRRRQGALLTA"
     gene            complement(1717..2706)
                     /locus_tag="PK829_00120"
     CDS             complement(1717..2706)
                     /locus_tag="PK829_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU39654.1"
                     /translation="MNTPIVTARNLRKTFGDFEAVKGISFSVLRGEIFSLLGPNGAGK
                     TTTINMLSCLLEPTAGTAEIAGHAIPGDTLGVRRAIGVVPQEIALYDALTARQNLEFW
                     GRLYDMRGQALARRVDEVLEQVGLLDRAQDKVKTFSGGMKRRINIAAGLLHKPQLLFM
                     DEPTVGIDPQSRRRILDMVKELRDGGMTVLYTTHYMEEAEELSDRVGIIDQGQLIALG
                     TQAELTRVVGEQETLRLHLAEEAAAEVAAVGGDRAAASPDGGLSLFNGLPGVLRVSTV
                     DHQIVVNVADAGAALPGVVGRANTLGLHVRSVDIEEPNLEAVFLHLTGRGLRN"
     gene            complement(2801..3343)
                     /locus_tag="PK829_00125"
     CDS             complement(2801..3343)
                     /locus_tag="PK829_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39655.1"
                     /translation="MLGGLLLTLGGILALIAQFVPDRWGFSFGVVVLLGLGAAFIIVG
                     ILTRAPGWFIPGGILTGIGAGVALTDGPLARLLPANVLPGDDGGLFMLAFAGGWFLIT
                     LLTALFTDRTQWWPIIPGTIMALIGLAAGFGSIFATTLTLLGKLWPIALILVGLYVLF
                     AGRRSSHHDADEAEKFVEKA"
     gene            3662..3898
                     /locus_tag="PK829_00130"
     CDS             3662..3898
                     /locus_tag="PK829_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39656.1"
                     /translation="MTDELPSKPTLRIREALNELLQRNDHTHGREVGEMYLGSDLFIA
                     RLRRPCIIEGQWEDTERTVDLFDLIDNHQRGDEE"
     gene            4003..4815
                     /gene="mutM"
                     /locus_tag="PK829_00135"
     CDS             4003..4815
                     /gene="mutM"
                     /locus_tag="PK829_00135"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOU39657.1"
                     /translation="MPELPEVETFVRALRRPLLGRTIIDLRNDWPRHIAQPAPDELAA
                     RIAGRRIEAIDRRGKYLVFTLSDDETLVIHLKMSGHLSVVPGDAPPDPYAHTVFLLDD
                     GGELRFRDPRKFGRVYLLRDPDGVLGPLGPEPLADDFSAEALAAALARRRRVLKPLLL
                     DQTFIAGIGNIYADEALFDAGLLPTRHSHTLTPDEVAALHAAIRKVLALGIAREGASI
                     STYVKPDGQKGDMQNAVAVFRRTGQSCYVCGGPIERIVLGGRSTHYCPNCQH"
     gene            4939..6024
                     /locus_tag="PK829_00140"
     CDS             4939..6024
                     /locus_tag="PK829_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HOU39658.1"
                     /translation="MNQDDIAHARLANQRLTETTFTTAAEVVAWLVAVQAQDYATAKW
                     ALGQRLPPTTDAAIEQAFNEGLILRTHVLRPTWHFVAPDDIRPLLALTAPRVHQANGS
                     MYRQLGLDATMLSRSAEVIARALDGRALTRDELKSALDEAGIAAGGTDRAGQRLAYIV
                     MHAELEGLIVSGPRRGKQFTYMLLDERAPGGRALARNEALVAVARRFFLSHGPATPAD
                     FANWSGLTLTEARQGLDAVAGELDSETVDGQTYWFADDPLPPRSPSPTVYLLSIYDEY
                     TIGYKDRRAIVSAEDGARLATMGNALQNVIVMDGRIVGTWRRTLAKTSLVIELNLFRV
                     LSEEERAAVEVAGQKYSEFLALSLALK"
     gene            complement(6035..6145)
                     /locus_tag="PK829_00145"
                     /pseudo
     CDS             complement(6035..6145)
                     /locus_tag="PK829_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
     gene            complement(6205..7827)
                     /locus_tag="PK829_00150"
                     /pseudo
     CDS             complement(6205..7827)
                     /locus_tag="PK829_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            complement(8422..10155)
                     /locus_tag="PK829_00155"
                     /pseudo
     CDS             complement(8422..10155)
                     /locus_tag="PK829_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            complement(10295..11236)
                     /locus_tag="PK829_00160"
     CDS             complement(10295..11236)
                     /locus_tag="PK829_00160"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOU39659.1"
                     /translation="MYGDIKLYAGSACPELAQRVASYIGIRLSRWDITTFANENIFIR
                     LNSSVRGQDVYLIQTHSKPIHSNIMEMLIAIDCFKRDSAGRVTVVIPYMSYSQSDKKD
                     QPRVPITARLLADMIEIAGADRWVTIDLHAGQIQGFYKIPGDSLTALHIICKHIRAKN
                     LNGTVVTTDLGFAKGGRNYAEALGFPLAFVEKRRVGAAGTREALTLIGSVAGRDVLIV
                     DDLVDTAGSVQQAVEIVRAHGAGDVYLAFTHAVLSPPAIERLGSLGLKEIITTDTLPV
                     APNDRLPNMTVLTVAELLGEVIIRSHEGRSVGELFNE"
     gene            complement(11255..12304)
                     /gene="meaB"
                     /locus_tag="PK829_00165"
     CDS             complement(11255..12304)
                     /gene="meaB"
                     /locus_tag="PK829_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU39660.1"
                     /translation="MDWIAEARSGNPRAIARLITQVERGAAEAQTIVRALYAATGRAH
                     IVGVTGPPGSGKSSLVNELAKAFRPRGRRVGIVAVDPSSPFTGGALLGDRVRMRDLSG
                     DEGIFVRSMASRGSLGGLARGVAAAVKVLDASGYDFILIETVGAGQAEVDIAGAAHTV
                     VVLEAPGAGDDVQSIKAGILEIADILVVNKADLLGANRTIRSLEMMLPLGPGAADDSS
                     HHALSDGHDGHDSHGSLPARWAVPVLPTIATTGQGVAELAATIVQHGDYLRASGGWAA
                     REQARSRDEIEHLLSDRFIDRLAAAVPAEERAALIAAVAERRLDPYTAADRLFAGAAA
                     ALAGAATPLAGSAGI"
     gene            12655..14154
                     /locus_tag="PK829_00170"
     CDS             12655..14154
                     /locus_tag="PK829_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU39661.1"
                     /translation="MNNNTLSRSRLPLFVALLIAVAVAGLSLGLRRIQAQAAQPTAVP
                     APVTFEARVDVSSLNLRTGPHVSYTAVAYLVDGEQVKLVGRNRAATWAQVELYNGYRG
                     WVNARYLQPGVNIAALPVADVALLGITAFVTNDSIPVYAGPDALYQRVGTGQPGDVLT
                     LDGRNDAATWVHVSLPGGPAGWARADSPFLPSASIHDLPIITPFLDAPPPELSAFYRV
                     YAGPGFLYEPVDSVVEGQTLGIRGRSDDARWLLVRLENGREGWIAAEILRVDMALEAV
                     PVIEGIAPPQVVGWQMSSPSSAAATATATSAPPTATGPMPATATAEATTGGKGTAPSP
                     ATATPGATPTGGGKGTAPNLDTATPAPGATATATTAPTERATAQPTARATATRPATPT
                     PAPTVAPTATTAPTQPPPTAAATTTTPPQTTGNPPPTGLPVVYVYATPSDANAPILRV
                     VPGQSVVLIGRTADSEWVRIWLPGDQQGWIRASAVQVEIDLAVLPVVAP"
     gene            complement(14620..16125)
                     /locus_tag="PK829_00175"
     CDS             complement(14620..16125)
                     /locus_tag="PK829_00175"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU39662.1"
                     /translation="MSLDLINIIFGMKVRQARLEAGLTLTDLARQAEMSPSYLTEIEK
                     GRKYPRADKIVKMADALGKAYDDLVSIRLAPSLQQLETTLSSATMQRFPFEEFGLEPA
                     DVLGLITREPERASALLHAVVEIARRYDLREEEFLRAALRSYQEMHENYFPDLEEAAL
                     RFSAEFGPRYGFGETTLPTLDTLTTLLRQEYDYSIDDRALANTEELRAFRSLLLPGRR
                     PQLFINSRLYARQVKFILAREIGYRYLSLKERSLISTPDRIDSFQQLLNDARAAYFGG
                     ALLMPRVRVLADLQAFFALPTWEPARFSAMLTTYEVTPEMLLYRFSELIPEFFGIKLH
                     FLRFHHTTGTDEYHLVRHLNMNQLLVPSGIGLLEHHCRRWLSIRLLPDTPGAAVDPLP
                     VGIQLSEFLSTQEQFLCLGFGRRMVLAPQVASSVIIGFRVDADLRATIRFLDDPNIPR
                     DIIHETCERCPLTPEQCAVRAAPPTLLEARREQLVRKLALSQLQARYGTGD"
     gene            16451..18040
                     /gene="aceB"
                     /locus_tag="PK829_00180"
     CDS             16451..18040
                     /gene="aceB"
                     /locus_tag="PK829_00180"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HOU39663.1"
                     /translation="MVKADNLLIQGEVSAEFASILTPEALAFVAELQRAFNGRRLELL
                     ARRVERQQRIDAGVMPDFLPETAGIRAAEWTVAPIPADLQDRRVEITGPVDRKMIINA
                     LNSGASVYMADFEDSHAPTWAATIEGQLNLRDAVNRVISFTSPEGKAYALNEKTAVLL
                     VRPRGWHLSEKHVLLDGEPLSGALFDFGLYFFHNVHNLLARGTGPYFYLPKLESHVEA
                     RLWNDVFVLAQERLGVPQGTIRATVLIENILATFEMDEILWELREHSAGLNCGRWDYI
                     FSAIRKFQKHPAFFLPDRAQVTMTVPFMRAYSQLVIKTCHRRNIHAMGGMAAQIPIKN
                     DPEANEAALAKVRADKEREARDGHDGTWVAHPGLVPIARDIFDRYMPTPNQIFRKRED
                     VHVTAADLLAIPTGTITAAGLRTNIDVGIQYMAAWLSGNGCVPIYNLMEDAATAEISR
                     SQIWQWLHNPNARLDDGRAITRDLFRGLTAEVLAELRARVGEADFVNNHYELAARLFD
                     EISVADQFTDFLTLKAYEYLN"
     gene            18188..19498
                     /gene="aceA"
                     /locus_tag="PK829_00185"
     CDS             18188..19498
                     /gene="aceA"
                     /locus_tag="PK829_00185"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HOU39664.1"
                     /translation="MFKPYNVAKLKDDWELNKRWQGITRPYSAEDVIRLRGSVQIEYT
                     LARMGAERLWRLMQQEPFVPALGAITGNQAVQQVQAGLKAIYLSGWQVAGDNNTAAQT
                     YPDQSLYPADSVPNMVKRINNALMRADQISHMNEEEGMYWFAPIVADAESGFGGSLNA
                     FELMKAMIEAGAAGVHFEDQLSSAKKCGHMGGKVLVPTREFIQKLVAARLAADVMGVP
                     TVLIARTDANGAQLLTTDIDPNDVPFIYGERTVEGFHRIHGGIECAISRGLAYAPYAD
                     VIWCETSHPDMAEARQFADAIHAKFPGKLLAYNCSPSFNWRRNLSESEIANFQKELGK
                     MGYAFQFVTLAGFHTLNASMFELSVGYRETGMAAYSHFQQREFEMEHEHGFRAIKHQS
                     FVGAGYFDEVQIAVSQGESSTVALKGSTEEEQFDEETPLVHYPN"
     assembly_gap    19884..19983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20309..20617
                     /locus_tag="PK829_00190"
     CDS             20309..20617
                     /locus_tag="PK829_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39665.1"
                     /translation="MAESDLRHYRQHGATGTTKQLLAALTSTGVAGLTLLDIGGGIGI
                     IQHELHAAGVVETTDVDASRAYLAIARREADRRGYGSDAHYIHGDFVAWPIGSRRPTS
                     "
     gene            20650..20898
                     /locus_tag="PK829_00195"
     CDS             20650..20898
                     /locus_tag="PK829_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39666.1"
                     /translation="MEALMHGAAARARRLLGLVYPRDGWWTKVGVTLLNLYPRLRGDA
                     FRSFAHPTAAVEAVAASNGLRRITHHEGLIWQVVVFAR"
BASE COUNT         3718 a         6971 c         6928 g         3568 t
ORIGIN      
        1 ggtgggcagg gccaacacga ccacgtcggc cggctgtccg gcggccaggc cgtcggccgt
       61 cagcgccgcg ccgaagccgg cggggatgac cagcacggcg gcgacagctt catcgtccag
      121 cagcgcctcg gcattggccc gccccatcac ttctggccgg acggtgtcgc cagcggccag
      181 cgcggccacg agggcagccg agcgcgctgt gccgtcctcg tcggccacag gcaggaagat
      241 gcgattgtcg ccgccgctgg ccgcgggcat accgccaccg ataagaaagg tgaacaccag
      301 cggcaggatc aggaagaaca gcagttccga acgcccctcg aagcggagga gcgtatcttt
      361 gcgaatgatg gcggccagtt tattgagcat tgtcgtcacc tgatgagcgc gagcattggc
      421 cgctaagctg tcaggagcgc cccctgccgg cggcggaaga gcagcgccgc ggcagcgaaa
      481 agcaccacgg ccatgaccag cagcgcggcg acgggcacga ggacggccgc gccaccgtcg
      541 cccgcggcca gcgccgtgaa gccgtccagc gcccagcggt tgggcgtgat gcggctgagc
      601 cagtcgagcg ccggccaggc gtcgttcagg ggcacgaagc tgccacccag gatgccaaag
      661 agcagcgtca gggccatgcc catgttggtg atctggccgg agttggcggc gacggcggcg
      721 atgagcagcc cccaaccggt ggcggccagg gccgcggcca cgaccagcag ggccacgctg
      781 agcgggttgc cccagttcag ccggaacagc agcgtcgtca ggaggatgag cgctgccagt
      841 tggatgaagc cgcccaggaa gatgccgccg accttgccgc cgagcacctg cgcgctgctg
      901 accggcgcgg ccagcatccg cgccagcgtg ccctcgcgtc gctccgacag cagcgtccgc
      961 gcgcccaggg tgacggcgta catcagaaac agcagggcca tgcccggcgc gaagtaggcc
     1021 agggggttaa aaggctggcc cgtagcggtc acgttgcgcg tctcgacacg gatgacggac
     1081 gcagtcgcgg ccacggcgcc atcggcaaag agcgcctcgc ccaggcgttg gccgagcgcc
     1141 ggcaactccg ccggcgacgc cgcgccgctg ctggccagtt gcgtcaggcc ggcgcgcaca
     1201 ccggccacgg ccgtgtcggc gcgggcagcg aacgcgtcga cgacgctgcg cacgacggcc
     1261 gcgcccaccg gcgcgcccgg atcaccgacg acgacggcgg cgacgggcgc gggcaactca
     1321 ccggtggcca tgtcgggcat gaaactgtcg ctgaagccgg ccgggatgag cacggcggcg
     1381 ctggcgtcgc cggcggccac gcgggcgcgg gcggcgtcag ggtcagtcag cgcctccacg
     1441 gcgagaaggt cgctcaggtc gttgcccgtc agcaggccgg ccagagccgc ggcgagctgc
     1501 ccgccgtctt catcgacgag gacgacggga atgcggccga tggccggcgc gtcgccgcca
     1561 ctgaacgcgc ccgtgaccag ccccatgccc agggccagca ccagcggccc ggcaatgatg
     1621 agagccaccg cggcccaatc cccgaacagg acgcgggcgt ctttccacat gatggtgagg
     1681 actttgttca tgatcgcctc tccagcgagc caggccctag ttgcgcaggc cgcggccggt
     1741 cagatgtagg aagacggctt ccagattcgg ctcctcgatg tcgacggagc gcacatgcaa
     1801 gcccagcgtg ttggcccggc cgacgacgcc gggcagggcc gcgccggcat cggcgacatt
     1861 gacgacgatc tggtgatcga cggtcgagac gcgcagcacg cccggcaggc cgttgaagag
     1921 cgacaggcca ccatcgggcg acgcggccgc cctgtcgccg ccgacagcgg ccacctcggc
     1981 cgccgcctcc tcagccaggt gcagccgcag cgtctcctgc tcaccaacga cgcgggtcaa
     2041 ctcggcctgc gtgcccaggg cgatgagctg gccctggtcg atgatgccca cgcggtcgga
     2101 cagctcctcg gcctcctcca tgtaatgggt cgtgtagagc actgtcatgc cgccatcgcg
     2161 caactccttg accatgtcga ggatgcgccg gcggctctgc gggtcgatgc cgacggtcgg
     2221 ctcgtccatg aacaacagtt gcggcttgtg gagcagcccg gcggcgatgt tgatgcgccg
     2281 cttcatgcca ccggaaaagg tcttgacctt gtcctgggcg cggtcgagca ggccaacttg
     2341 ctccagcact tcgtccacgc gccgggccag ggcctggccg cgcatgtcat agaggcggcc
     2401 ccagaactcc aggttctgcc gggcggtcag cgcgtcgtag agggcgatct cctgcggcac
     2461 gacgccgatg gcgcggcgga cgccgagcgt gtcgccggga atggcatggc cggcgatctc
     2521 ggccgtgccc gccgtcggct ccagcaggca gctgagcatg ttgatggtcg tcgtcttgcc
     2581 cgcgccgttg gggccgagca ggctgaagat ctcgccacgc aggacgctga acgagatacc
     2641 tttgaccgct tcaaagtcgc cgaaggtctt gcgcaggttc ctggcggtga cgatgggggt
     2701 gttcatgagg gcctccgagg acgatgtgtc gagtcttgcg gattggcgtt gagagaaaaa
     2761 ggcgcgtcac ccttcggaaa gagtgacgcg cctaccgggt tcaggccttt tcgacgaact
     2821 tctcggcttc gtcagcgtcg tggtggctcg agcgccggcc ggcgaagagg acgtagaggc
     2881 caacgaggat aagggcgatg ggccaaagct tgcccagcag ggtcagcgtc gtggcgaaga
     2941 tgctgccgaa gcccgcggcc aggccgatga gggccatgat ggttccgggg atgatcggcc
     3001 accactgcgt cctgtcggtg aagagcgccg tcaggagggt gatcaggaac cagccgccgg
     3061 cgaaggcgag catgaacagg ccgccgtcgt cgccgggcag cacgttggcg ggcagcaggc
     3121 gcgccagcgg gccgtccgtc agggcgacac ccgcgccgat accggtcagg atgccgccgg
     3181 ggatgaacca gcccggtgcg cgggtcagga tgccgacgat gataaaggcc gcgcccaggc
     3241 ccagcaggac cactacgcca aagctgaagc cccaacgatc gggtacgaat tgagcgatca
     3301 gggccagaat gccgcccaag gtgagcagca gaccgccgag cataggatta cggttttcgc
     3361 gaatgcgatg ggagcgggtg gacatgacaa ctacctcttt gcatctgaac tgtcgaacgt
     3421 ggatcaactg tcactagaat agcattccgg ggtgatgata ggatgtgccg gaagtcatgt
     3481 cagggtcatg cgggtgtcat ggttagccgc gccggcccgt gcttatgcgc gactcatatc
     3541 gcgacccgcg cccactctgc cgacgcgaat ttatacgcca ttttcacggc acgcgccagg
     3601 cgcaggacta caatgcagga cagggtgtgg tgaaagtatg ggaagaggcc gagggacaac
     3661 aatgacagac gaactgcctt cgaagccgac gctgcggatt cgcgaggcgc taaacgaact
     3721 cttgcagcgc aacgatcaca cccacggacg cgaggtcggt gaaatgtacc tcggttccga
     3781 cctgttcatc gcccgcctgc gccgcccctg catcatcgaa ggacaatggg aagacaccga
     3841 acgcaccgtt gacctcttcg acctcatcga caaccatcag cgcggcgacg aagaataaaa
     3901 cgctcacgcg aaggcgcaaa ggagataaaa gttcttcttt gcgtcctttg ccctcttcgc
     3961 gtcctttgcg tgagcctctt cgttctcggc tataatcggc ccatgcccga gttgcccgaa
     4021 gttgaaacct ttgttcgcgc gctgcggcgg ccgctgctgg gccgcaccat cattgatctg
     4081 cgcaacgact ggccgcgcca catcgcccaa cccgcgcccg acgagctggc ggcgcgcatc
     4141 gccggccggc gcatcgaggc catcgatcgc cgcggcaagt atctcgtctt cacgctgagc
     4201 gacgacgaga cgctggtcat ccacctgaag atgagcggcc acctgtcggt cgtgcccggc
     4261 gacgcgccgc ctgaccccta tgcccacacg gtcttcctgc tggacgacgg cggcgagttg
     4321 cgcttccgcg acccgcgcaa gttcggccgc gtctacctgc tgcgcgaccc cgacggcgtg
     4381 cttggcccgc tcggcccgga gccgctggcc gacgacttct cggccgaggc gctggcggcg
     4441 gcgctggccc ggcggcggcg cgtgctcaag ccgctgctgc ttgaccagac cttcatcgcc
     4501 ggcatcggca acatctatgc cgacgaagcg ctgttcgacg ccggcctgtt gcccacccgc
     4561 cacagccaca ccctgacgcc cgacgaggtc gccgccctgc acgctgccat ccgcaaggtg
     4621 ctggccctgg gtatcgcccg cgagggggcc agcatcagca cctacgtcaa gcccgatggc
     4681 cagaagggcg acatgcagaa tgccgtggcc gtctttcgcc gcacggggca atcgtgctac
     4741 gtttgcggcg ggccaattga gcgcattgtc ctgggcgggc gcagcaccca ctactgcccc
     4801 aactgtcagc attagattgg atggaaatga aataagaacc tcacgcaaag ccgcaaagga
     4861 agccaagacg caaagaaaaa tgagattgct tccttcgcgt cctttgcgtg aggttcttcc
     4921 tgcaaagaca atccctcaat gaatcaagat gacattgccc acgcccggct ggccaaccag
     4981 cgcctgacag aaacgacgtt cacgacagcg gccgaggtcg tcgcctggct ggtggccgtc
     5041 caggcgcagg actacgccac cgccaagtgg gcgctgggcc aacggctgcc gccgacgacg
     5101 gacgcggcca tcgaacaggc cttcaatgag gggctgatcc tgcgcactca tgtgctgcgg
     5161 ccgacgtggc acttcgtcgc ccccgacgac atccggccgc tgctggcgct gaccgcgccg
     5221 cgcgtccacc aggccaacgg cagcatgtac cggcaactgg gccttgacgc gaccatgctg
     5281 agccgcagcg ccgaggtcat cgcccgcgcc ctggacggcc gggcgctgac ccgcgacgag
     5341 ctgaagagcg ccctcgacga ggccggcatc gccgccggcg ggacggatcg cgccgggcaa
     5401 cgcctggcct acatcgtcat gcacgccgag ttggaggggc tgatcgtcag cggcccgcgg
     5461 cgcggcaagc aattcaccta catgcttctg gacgagcgcg cgccgggcgg gcgggcgttg
     5521 gcgcgcaacg aggcgctggt ggcagtggcg cgacgcttct tcctgagcca cggcccggcc
     5581 acgccggccg acttcgccaa ctggtcgggg ctgacgctga ccgaggcgcg gcaggggctg
     5641 gacgcggtcg ccggcgagct ggacagcgag acggtcgatg ggcagaccta ctggttcgcc
     5701 gatgatcctc tgccgccgcg ctccccgtcg ccgaccgtct atctgctgtc aatctatgac
     5761 gagtacacca tcggctataa ggatcgccgc gccatcgtca gcgccgagga tggcgcgcgg
     5821 ctggcaacaa tgggcaatgc cttacagaac gtgatcgtca tggatgggcg gattgtgggg
     5881 acgtggcggc ggacgcttgc gaaaacatcg ctcgtcatcg aactcaatct cttccgggta
     5941 ctaagtgaag aggaaagggc ggctgtggaa gtagcagggc aaaagtatag tgagttcttg
     6001 gcactatctc tggcactgaa gtaataacag gagtggtatc cttaatcgtc cgcccctgag
     6061 ctaccaaacc tggaatgtcg tccgatgtag ccaggtacac accctccggg agtttttcaa
     6121 tgtgaagttt ggcaactcgc tccatccatc ctccgcctgc atcatagaaa ccgagttcct
     6181 cagaaggaac tcggtttcta aaacttaccg ccgcgccttc ttcttctgcg gctgcggctt
     6241 cgccttggcc gcgggctggg cgatggtctg gccgttgcca ccgccgccgg ccagcatggc
     6301 cgcgcccggc cggccgtggc agttcttgta cttccggccg ctgccacacg ggcaggggtc
     6361 gttgcggccc acgtccgggc tgtccttgcg cacttcggtg cccttgccct tctcgcgctt
     6421 gacggtctgg tagccggcgt cctgcgactg caccttgtag gccacttcct gctgctgttg
     6481 ctggatgaaa atctggtgat cggcaatccc gcggaagaag cggtcggcaa tgtcgcggtc
     6541 gatgttcttg cgcatgtcgc cgaacatctc aaacgagcgc ttcttgtatt cgatcttggg
     6601 atcgcgctgg ccgaaggcct gcaagccgat ctcgcgccgc aggtcatcca tggccgtcag
     6661 atagtcgcgc cactcgcggt cgatggccga cagcagcagc agccgctcgt agttctggta
     6721 ctcctcgttg ccgatgagct gccgccactt ggcctgctgc tgatcgatgt actgctccag
     6781 cgccgtagcc agctgctcac cattcagttc ctgcaaactc tgcaccagca aatcgctcag
     6841 aacgcggtct acctcggcgc ggaagtggcg ctggcgctcc tccatgttct tgaccgacag
     6901 gccgtcgagg ctgaactggc tgaaagcgcg gtttagctcg ctctccgcct cggcccagat
     6961 cggctcgcgg tcggcctggt caatcgtctc ggccaggaac tcgttgtaga gcatctgcac
     7021 ctgggcgatg aagccggcgc gcaggcggtc gcgggtctgc acctggccac ccttgtgcgt
     7081 cgccagcgcg cccagattgg gcacgggcgg caccagcggc aggaagcggc ccatcgcctg
     7141 tagcaactgc aataggttcg tgccctggcg ctcctgctcg tgggccaatg aggccagccg
     7201 tgaggtcagc cggtcgggcg acaggcgctc caactcgtcg cgctccagat cggccagcag
     7261 gggcagcatc ggccccagcc ggcgcaaaac ggcggccacg ttgaccgtgt tggtggcgtc
     7321 ggtggtgaag tcgttgacga cgcgctccac ttcgccgcgg atgtagctgg ggtaggcgtc
     7381 gatgtagttc tctaccaggg cggcgatggc gtcatcgaag gcagcatcca ccttggcatc
     7441 caggtcgacg tcaccgccca tgaggatggc gcggcgctcg ccgtagatgg cctggcgctg
     7501 cttgctcatc acgtcgtcgt actcgacgac gttcttgcgc atgtcgaagt tgtagccctc
     7561 gacgcgctcc tgcgagcttt cgatgatctt gtccagcaga ccgctctcga taggcatgtc
     7621 ctccggaatg ttggtgcggg acatgaagcc cttcagccgt tcgccgccga agcgccgcat
     7681 caggtcgtct tccagcgaga ggaagaagcg cgatgaaccg gggtcgccct ggcgggcggc
     7741 gcggccgcgc aactggttgt cgatgcgccg cgactcgtgc cgctcggaac cgacaacgtg
     7801 cagaccgccc aaccgccaga tgtcttcctt atcgcggcgg gcctgggcgc gcaactcctc
     7861 aatgcggctg atgtgctgtg ggccgaggcc agggatggtc tccaccagcc gccgggcttc
     7921 gtcgggccgg tcgcccaaca cggcgcggat gagcgcggcg cgggcgttgt agtggcgctc
     7981 aaagacctgt tcggccagga attgcgcggc cagcttttcg tcagcgtgga tgttctgcca
     8041 gcggatgagt tctgtctgac ggcgcaaaat ctcatcgacc aaagccacgt cctggtccag
     8101 gctctctaca aaggctcgcg cctgggccag ttcgccagtg cgcaccaggc gcagcgtcgt
     8161 caccaggtcg ttgtagtcga tgctatacgg ctcttgcagc acacgggcca ggtagcccat
     8221 gacctgcact tgctcgatct cagtcatggc ggcgtcgaag cggtcgcggg tctccagcag
     8281 agcatcgaca aggtcttcgg tcagccggtt gctcttgcgg gcgatgtcgc gggcggcctc
     8341 ttgcccttcg ctcacgagct tgagcgccag ctgggccagc atcggccggt cgaacatctc
     8401 gctttccaac gcttcggcgg ccaacccctc ggcgttgccg cccaggagga tgtcggtgcc
     8461 gcggccggcc atattggtag aaatagtcac ggcctgccgc cggccggcct gggccacgac
     8521 gagcgcctcg gactggtgca tcttggcgtt gaggacgttg tggcggatgc ccgcgcgggt
     8581 cagcagcttg gcaatcgtct ccgagtgctc gaccgaggtt gtgccgacca gaatgggccg
     8641 gcccgtctgg tggacgcgct tgatctcgtt gatgatggcc tggtctttgg cctcgctcgt
     8701 gccgtagacc tggtcggccg agtccacgcg cttatagtag accggctcgc ccgtctgcgg
     8761 cttgacgtag accacagcct ccgcgccatc gaccttctcg cggcgcgtct ccaaccccat
     8821 ctggcccgtc tcgatgatgt actcgacgtt ggtcggcagc ggcgtgacgc ccagctcgta
     8881 gatctcctcg aattcctcgg cgtcggtcag ggccgtaccg gtcatgccgg ccagcttgtc
     8941 gtagaggcgg aagtagtttt gcagcgtgat ggtcgccacc gtcaccgtct cgcgctgcac
     9001 atcgacgccc tccttggcct cgatggcttc gtgcaaaccg tcggagtagc gccggccggg
     9061 catgaggcgg ccggtgaagt catcgacgat gatgacctca tctccctgga cgacatagtc
     9121 cacgtcgcgc ttatagagat actgggcgcg cagggcattg tcgagatagt aggtcagatg
     9181 gtagaaacgc gggtcataga ggctgtcgcc ggcgtcgggg tccatctcct cgatgcgctt
     9241 ttcgatttcg ctcaggccga tttccgtcag gctgatgctg cggctcttct cgtcgatgtc
     9301 gtagtggccg ttggggtctt catcttcgtc ggccgtgttg gggcgcaggc ggcggacgta
     9361 ctcggcaaag cgggagtagt ccttgctgct gcgcgccgcc ggcccggaga tgatcagcgg
     9421 cgtgcgcgcc tcgtcaatga ggatgttgtc aacctcgtcg atgatggcgt agttcaggcc
     9481 gcgctggacg agttggtcct ggcgcacggc catgttgtcg cgcagatagt cgaagccgaa
     9541 ctcagagctg atgccgtagg tgatatcggc ctgataggct tgctcgcggt tgcacggccg
     9601 ccagtggacg agccgctcat cttccaactc agcgccgggg ttgacgtact ccgggtcgaa
     9661 cagggccgag aactgcaacg ggccgatgac gccgacagac aggccgagag cgtggaaaat
     9721 cttgcccatc cagccgccgt cgcgccgggc caggtattcg ttgactgtga ccagatggac
     9781 gccgttgccg gtcagggcat tgagatacag tggcagcgta gcgaccagcg tcttaccctc
     9841 gccggtgcgc atctcgacaa cctcaccctg gtggagcaac atgccgccca tcagttgcac
     9901 gtcataggga cgcaacccga tggcccgccg cgacgcctcg cgcacaaggg caaaggcctc
     9961 gttgaggacg tcatctagct ccgccccgcc ggccacccga tccttgagcg ccagcgagcg
    10021 ctcgcgcagg gcgtcagcgg acagcggcgt caattgcggc tccagcgcgt tgatagccgc
    10081 cacgctgggc ttcaggctat tgatgatctt cttgttggca tcgccgacga tgctggaaac
    10141 gattttcttg aacatggcaa gacctattat agtgaacgac gcaagggcaa ggcagctttc
    10201 ttaacccgcg tcttgcctga gggaaggatg gcccgtctgt gcgggcgcgc cgggcgaccg
    10261 gccaagccgc cgcgaagcac aaccaccagc cggcttactc gttgaacagt tcgccaacac
    10321 tgcgcccctc atgcgagcgg ataatcacct cacccagcag ctcggccacg gtcagaacag
    10381 tcatgttggg cagccggtca tttggggcaa cgggcagggt gtcggtggta atgatctcct
    10441 taaggcccag gctgcccagc cgctcaatgg ccggcggaga cagaacggca tgggtgaagg
    10501 ccaggtagac gtcgcccgcg ccgtgggcgc ggacgatctc aacggcctgc tggacggagc
    10561 cggccgtgtc gaccaggtcg tcaacgatga gaacgtcgcg gcccgccacg ctgccgatga
    10621 gcgtcagggc ctcgcgcgtg ccggccgccc ccacgcgccg cttctcgacg aaagccagcg
    10681 ggaagcccag cgcttcggcg tagttgcgcc cgcccttggc gaagcccagg tcagtggtga
    10741 cgaccgtgcc gttgaggttc ttggcgcgaa tgtgcttgca gatgatgtgc agcgcggtca
    10801 gcgagtcgcc gggaatcttg tagaagccct gaatctgccc ggcgtgcagg tcaatagtca
    10861 cccagcgatc ggccccggcg atttcgatca tgtcggccaa caggcgggca gtgatgggca
    10921 cgcgcggctg gtctttcttg tcgctctgcg agtagctcat gtaggggatg accaccgtca
    10981 cccgcccggc cgagtcgcgc ttgaagcagt cgatggcgat cagcatctcc atgatgttgc
    11041 tatggatggg cttgctgtgg gtctggatca ggtagacatc ctggccgcga acgctgctgt
    11101 tgagccggat gaagatgttc tcgttggcaa atgtggtgat gtcccaacgg ctaaggcgga
    11161 tgccgatgta gctggccacg cgctgcgcca gttcgggaca ggcggagccg gcatagagtt
    11221 tgatgtcgcc gtacatgtag gatgctccag agtgctagat gccggctgaa ccggccaagg
    11281 gggtcgccgc gccggccagg gcggcggccg cgccggcgaa gaggcggtcg gccgccgtat
    11341 aggggtcaag gcggcgctcg gccacggcgg caatgagggc ggcgcgctct tcggccggca
    11401 cggccgccgc cagccggtca atgaaacggt cactcagcaa atgctcgatc tcgtcgcggc
    11461 tgcgcgcctg ttcgcgcgcc gcccagccgc cagacgcgcg cagataatcg ccgtgctgga
    11521 cgatggtcgc cgccaactcg gccacgccct ggccggtcgt ggcaatggtg ggcaacacgg
    11581 gcacggccca acgggcgggc aggctgccgt ggctgtcgtg gccgtcgtgg ccgtcgctga
    11641 gggcgtgatg gctgctgtcg tcggccgcgc ctggccccag gggcagcatc atctccagcg
    11701 accggatcgt ccgattcgcg cccaagaggt cggccttgtt gaccaccagg atatcggcaa
    11761 tctccaggat gccggccttg atcgattgca cgtcgtcgcc ggcgcccggc gcttccagca
    11821 cgacaacggt gtgggccgcg ccggcaatat cgacctcggc ctggcccgcg ccgacggtct
    11881 cgatgagaat gaaatcgtag ccagaggcgt cgagaacctt gaccgccgcg gcgacgcccc
    11941 gcgccaaccc gcccaggctg ccgcgcgagg ccatgctgcg gacgaagatg ccctcgtcgc
    12001 cggacagatc gcgcatgcgc acgcggtcgc ccagcagcgc cccaccggtg aacgggctgc
    12061 tagggtccac ggcgacgatg ccgacgcgcc ggccgcgcgg ccggaaagcc ttagccaact
    12121 cattgaccag gctgctcttg ccggagccag gcgggccggt aacgccgacg atgtgggcgc
    12181 ggccggtggc cgcgtagagg gcgcgcacaa tcgtctgcgc ctcggcggct ccacgttcga
    12241 cctgggtgat gagccgggcg atggctcgtg ggttgccgct acgggcctcg gcgatccaat
    12301 ccatagtaat ctttcccgca agaaccgccg gatattatag ccctggggcg gacgttttac
    12361 gaacggacgc ggacatgggc acggctattt agtggtcgct ggtggcccgg agacctgaca
    12421 ggtgcgcggc cgaagtctct cccgcagaaa tgagccgccg ggtcgccacc ggtcaggtcg
    12481 aggcgctcgt atcgacgaat gaaaagccca cgcccatgaa tgcggcgcgc ctgtgaaaaa
    12541 tgtgatcata ttggccgccg taccttttcg gcaagcgtcg ccgctcctat actgcaccag
    12601 tagagggttt ggcagtaccg ataagcccca gcaacctaaa gagtcgtatt ggccatgaac
    12661 aacaacaccc tctctcgctc tcgcttgccg ctctttgtcg ccctgctgat cgctgtggcg
    12721 gtcgccggcc tgtcgctggg cctgcgccgc atccaggcgc aggcggccca gccaacggct
    12781 gtccccgcgc cggtcacctt cgaggcccgc gtcgacgtca gttcgctcaa cctgcgcacc
    12841 gggccgcacg tcagctacac ggctgtggcc tacctggtgg acggcgagca ggtcaagctc
    12901 gtcggccgca accgcgccgc cacctgggcg caggtagagc tatacaacgg ctatcgtggc
    12961 tgggtcaacg cccgctactt gcaacccggc gtcaacatcg ccgcgctgcc cgtggccgac
    13021 gtggccctgc tgggcatcac cgcctttgtg accaacgact ccatccccgt ctacgctggg
    13081 ccggacgcgc tctaccagcg cgtgggcaca ggccagccgg gcgatgtgct gaccctcgac
    13141 ggccgcaacg acgccgccac ctgggtgcac gtcagcctgc ccggcggccc ggccggctgg
    13201 gcgcgcgccg acagcccctt cctgccctca gccagcatcc atgacctgcc catcatcacc
    13261 cccttcctcg acgcgccgcc gccggaactg tcggcgttct accgcgtcta cgccggcccc
    13321 ggcttcctct atgagccggt cgatagcgtc gtcgaagggc agacgctggg cattcgcggc
    13381 cgctctgacg acgcgcgttg gctgctggtg cgtctggaga acgggcgcga gggctggatc
    13441 gccgcggaga ttctccgggt agacatggcc ctggaggccg tgcccgtcat cgagggtatc
    13501 gccccgccgc aggtcgtcgg ctggcagatg agcagcccca gcagcgccgc ggcgacggcg
    13561 acagccacca gcgccccgcc cacggccaca gggccgatgc cggcgacagc cacggccgaa
    13621 gcgacgaccg gaggcaaggg cacagccccc agtccagcca cggctacgcc gggggcgaca
    13681 cccacgggcg gcggcaaggg cacggccccc aacctcgaca cagccacgcc agcgcccgga
    13741 gccacggcta cggccacaac tgcgccgact gagcgtgcca cggcccaacc aaccgcacgg
    13801 gcgaccgcga cacggccggc cacgccaacc cccgcgccga ccgtcgcgcc gacggccacg
    13861 accgcgccaa cccagcctcc gcccacggcc gcggccacga ccacgacgcc cccgcagaca
    13921 accggcaacc cgccgcctac cggactaccc gtggtctacg tctatgccac gccgtcggac
    13981 gcgaacgcgc ccatcctgcg cgtcgtgccc ggccagagcg tcgtcctcat tgggcggacg
    14041 gccgatagcg aatgggtgcg catctggctg cccggcgatc aacagggctg gatccgcgct
    14101 tcggcggtgc aggtggaaat cgatctggct gtcctgccgg ttgtggcccc atagcgccta
    14161 gcgtggtgcc gaagaaaaca attcccatag aatcacattt tctatcttct tatttagtag
    14221 tcgtagtagg ggctggggaa acgtggataa ctgccacgcc tgcccatata tagggggtgg
    14281 tggccgcaaa tgccgggcac gtaggcctta tgtggcgggg cggggcggcg gggcggtgga
    14341 tggactgttg gtaagcccgg ggcgaacgtt tgtcctagca gttgctgagt ctgcccatgt
    14401 ccgggggcat ctcagaccac tgcaccccta tatatagcca tttcggccgg ccggatgccc
    14461 atataaagat gagcgcgcgc cgccacgccc ggcggacgcg cgtccagatt tccccagtgg
    14521 ccgatctgtt ttccacagct ttgtgcgagt tatccacacc tcagtgcccg cgctttctga
    14581 ggcgctggcc ggaaggtatg gccctgtgcc cgacaattgc taatcgcccg tgccatagcg
    14641 cgcctgaagt tgactgaggg ccaacttgcg caccaattgc tcgcggcggg cctccagcag
    14701 ggttggcggc gcggcgcgga cggcgcattg ctccggcgtc agcgggcagc gctcgcaggt
    14761 ctcgtggatg atgtcgcggg gaatgttggg gtcgtcgagg aagcggatgg tcgcgcgcag
    14821 gtccgcgtcc acccggaagc cgatgatgac gctgctggcc acctggggcg cgagaaccat
    14881 gcgccggcca aagcccaggc acagaaattg ctcctgcgta gacaggaact cggatagctg
    14941 gatgcccact ggcagcgggt cgaccgccgc gcccggggtg tccggcagca ggcgaatgga
    15001 cagccagcgc cggcaatggt gctccaacag gccgataccg ctgggcacaa ggagctggtt
    15061 catgttcagg tggcgcacca gatggtactc gtccgtgccc gtggtgtggt ggaagcgcag
    15121 gaagtgcagc ttgatgccaa agaactccgg gatcaactcg ctgaagcggt agagcagcat
    15181 ctccggcgtg acttcgtagg tggtcagcat ggccgagaag cgcgccggct cccaggtggg
    15241 cagggcgaag aaagcttgca ggtcggccag cacccggacg cgaggcatca gcagtgcccc
    15301 gccgaagtag gcggcgcggg cgtcgttgag cagttgctgg aaggaatcga tccggtcggg
    15361 ggtggagatc agcgagcgct ccttcagaga aaggtagcga tagccgatct cgcgggccaa
    15421 gatgaacttg acctgccggg catagaggcg gctgttgatg aagagctgcg gccggcggcc
    15481 gggcagcagc agcgagcgaa aggcgcgcag ttcctcggtg ttggccaggg cgcggtcgtc
    15541 gatgctgtag tcatactcct gccgcaagag cgtggtcagc gtgtcgagcg tcggtagggt
    15601 cgtctcgccg aagccgtagc gtgggccgaa ctcggcgctg aagcgcaggg cggcctcctc
    15661 caggtcgggg aagtagttct cgtgcatctc ctgataggag cgcagggcgg cgcgcagaaa
    15721 ttcctcttcg cgtaggtcat agcgccgggc gatctcgacg acggcgtgga gcagagcgct
    15781 ggcccgttcc ggctcgcggg taatcaggcc cagcacatcg gccggttcca ggccaaactc
    15841 ctcaaacggg aagcgctgca tcgtcgcgga ggacagcgtt gtttccagct gttgcagcga
    15901 cggcgcgagg cgaatcgaca ccaggtcgtc gtaagccttg cccaatgcgt cggccatctt
    15961 gacgatcttg tcggcgcggg ggtacttgcg gcctttctcg atctcggtca ggtaagacgg
    16021 cgacatctct gcctggcggg ccaggtcggt cagggttagc cccgcctcca gccgcgcctg
    16081 gcgcactttc atgccgaaaa tgatgttgat gaggtctagg gacatggtgg gtcagtggtc
    16141 agtgggtcag taggtagtga acagtaaaca gtaaatagtg ggcggtgaag tcgtggtcgg
    16201 cggtcggcgg ccggcggttg gccaacatga cattcatcac tttacaactc atgacactct
    16261 gcgttataat gacgcagtgc gttagtaacg gcgcgcatca tctgggagtc attctagcga
    16321 attttcgcta attgccaaga aagagggtgt tttttcttga caaagagcgg ttcatgccct
    16381 tatactgccc aaaacattag cgaattttcg ctggcagtga agtttatcct gagtcaagga
    16441 gaggatcagc atggtgaaag cagacaacct tcttatccag ggtgaggtct cagcggagtt
    16501 cgccagcatt ctgacgcccg aggcgctggc cttcgtcgct gagttgcagc gcgccttcaa
    16561 cgggcggcgg ctggagctat tggcccggcg cgtcgagcgc caacagcgca ttgacgccgg
    16621 cgtcatgccc gacttcctgc cagagacggc cggcatccgc gccgccgagt ggacggtcgc
    16681 ccccatcccc gccgacctgc aagaccgccg cgtggaaatc accggcccgg ttgaccgcaa
    16741 gatgatcatc aacgcgctca actccggggc cagcgtctat atggccgact tcgaggattc
    16801 gcacgcgccc acctgggcgg cgaccatcga gggccagctc aacctgcgcg acgccgtcaa
    16861 tcgcgtcatc agcttcacca gcccggaggg caaggcctac gccctcaatg agaagacggc
    16921 cgtgctgctc gtccggccgc gcggctggca cctgagcgag aagcacgtcc tgctggacgg
    16981 cgaaccgctg tccggcgcgc tgttcgactt cggcctctac ttcttccaca acgtccacaa
    17041 cctgctggcc cgcggcactg gcccctactt ctacctgccc aagctggaga gccacgtgga
    17101 ggcgcggctg tggaacgatg tcttcgtgct ggctcaggag cgcctgggcg tgccgcaggg
    17161 gacgatccgc gccacggtgc tgatcgagaa catcctggcc accttcgaaa tggacgaaat
    17221 cctctgggag ttgcgggagc actcggccgg cctgaattgt ggccggtggg attacatctt
    17281 cagcgccatt cggaagttcc agaagcaccc ggcgttcttc ctgcccgacc gcgctcaggt
    17341 caccatgacc gtgcccttta tgcgcgccta ctcgcaactg gtcatcaaga cctgccaccg
    17401 ccgtaacatc cacgccatgg gcggcatggc ggcgcaaatc ccgatcaaga acgacccgga
    17461 ggccaacgag gcggccctgg ccaaggtgcg cgccgacaag gagcgcgagg cccgcgacgg
    17521 ccacgacggc acgtgggtgg cccaccccgg cctggtgccc atcgcccgcg acatcttcga
    17581 ccgctacatg ccgacgccca accagatttt ccgcaagcgc gaggacgtcc atgtgaccgc
    17641 ggccgacctg ctggccatcc ccaccggaac gatcaccgcc gccggcctgc gcaccaacat
    17701 cgacgtcggc atccagtaca tggccgcctg gctcagcggc aacggctgcg tgcccatcta
    17761 taacctgatg gaggacgcgg ccacggccga gatctcgcgt tcgcagatct ggcagtggct
    17821 ccacaacccc aacgcccggc tggatgacgg ccgcgccatc acccgcgacc tcttccgcgg
    17881 cctgaccgcc gaggtgctgg ccgagctgcg ggccagggtg ggcgaggcgg acttcgtcaa
    17941 caaccattac gaactggccg cgcgcctgtt cgatgagatc agcgtcgccg accagttcac
    18001 tgatttcctg acgctgaagg cttacgagta cctgaactag gcttgtaggg cgggttgcca
    18061 acccgcccta cgaacagagg cgggttgcca acccgcccta cgaacagagg cgggttgcca
    18121 acccgcttta cgaacagagg cgggttgcca acccgcccta caaacagaaa gaaatggaga
    18181 caagaggatg ttcaaaccgt ataacgtggc taagctgaag gatgattggg aattgaacaa
    18241 gcgctggcag ggaatcaccc ggccctactc ggccgaggac gtcatccgtc tgcgcggttc
    18301 ggtgcagatc gagtacacgc tggcgcgcat gggcgcggag cgactgtggc ggttgatgca
    18361 gcaggagccg tttgtgcccg cgctgggggc catcaccggc aaccaggccg tgcagcaggt
    18421 gcaggctggc ctgaaggcca tttacctgag cggctggcag gtggcgggcg acaacaacac
    18481 cgccgcccag acctaccccg accagagcct ctacccggcc gacagcgtgc ccaacatggt
    18541 caagcgcatc aacaacgccc tgatgcgcgc cgaccagatc agccacatga acgaggaaga
    18601 gggcatgtac tggttcgcgc ccatcgtcgc cgatgccgag tcgggcttcg gcggttcgct
    18661 caatgccttt gagctgatga aggcgatgat tgaggctggc gcggccggcg tccacttcga
    18721 ggaccagctc tcctcggcca agaagtgcgg ccacatgggc ggcaaggtgc tcgtgccgac
    18781 gcgcgagttc attcagaagc tggtggcggc gcggctggcg gccgacgtca tgggcgtgcc
    18841 caccgtcctc atcgcccgca ccgacgccaa cggcgcgcaa ctgttgacga ccgacatcga
    18901 ccccaacgac gtgccgttca tctacggcga gcgcaccgtc gagggcttcc accgcatcca
    18961 cggcggcatc gagtgcgcca tcagccgcgg cctggcctac gccccctacg ccgacgtcat
    19021 ctggtgcgag acctcgcacc cggacatggc ggaagcgcgc cagttcgccg atgccatcca
    19081 cgccaagttc cccggcaagc tgctggccta caactgctca ccgtcgttca actggcgacg
    19141 caacctgagc gagtcggaga tcgccaattt ccagaaggaa ctgggcaaga tgggctatgc
    19201 cttccagttc gtgaccctgg ccggcttcca cacgctgaac gccagcatgt tcgagctgtc
    19261 cgtgggctac cgcgagacgg gcatggccgc ctactcgcac ttccagcagc gcgagttcga
    19321 gatggagcac gagcacggct tccgcgccat caagcatcag agcttcgtcg gcgcgggtta
    19381 cttcgacgag gtgcagatag cggtttcgca aggggaatcg tcgactgtgg ccctcaaggg
    19441 ttcgaccgaa gaggagcagt tcgacgagga gacgccgctc gtccactacc ccaactaaac
    19501 cgcccgattc tgcttctcca tttcatttct ccttcagtga gagaggggtg gccgcccggc
    19561 cgcccctctc tcgcgttgtg gggcgatttg ccaaatcgcc ctacaggcca cgggccggtt
    19621 gccgaagacg cgccgcttgg ccaatcccgt agcccaactt ctcctgttgt ggggcgattt
    19681 gccaaatcgc cctacaggcc acaggccggt tgccgaagac gcgccgcttg gccaatcccg
    19741 tagcccaact tctcctgttg tggggcgatt tgccaaatcg ccctacaggc cacaggccgg
    19801 ttgccgaaga cgcgccgctt ggccaatccc cgtagcccaa cttctcctgt tgtggggcga
    19861 tttgccaaat cgccctacag gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnncgtagcc caacttctcc tgttgtgggg cgatttgcca aatcgcccta caggccacgg
    20041 gccggttgcc gaagacgcgc cgcttggcca atcccgtagc ccaacttctc ctgttgtggg
    20101 gcgatttgcc aaatcgccct acaggccaca ggccggttgc cgaagacgcg ccgcttggcc
    20161 aatccccgta gcgcagccag cgcccttaaa tccgggcttg cattcgacgc gcctaacctt
    20221 taccctggat gcggataccc tatatgcgga ggcgaagatg aattgttgcc cacactgcca
    20281 gggggcagac cgattcttca atcagcgcgt ggctgagagc gatctgcgcc actaccggca
    20341 acacggcgcg accggcacga ccaagcagct cctggccgcg ctgacatcga ccggagtcgc
    20401 cggcctgacg ctactcgata taggcggcgg catcggcatc atccaacacg aactgcacgc
    20461 cgccggggta gtcgagacca cggacgttga cgcctcacgg gcttacctgg cgattgcccg
    20521 ccgggaagcc gacagacgcg gctacggcag cgacgcgcac tatatccacg gcgattttgt
    20581 ggcctggccg atcggatcga ggcggccgac atcgtaacgc tggatcgcgt cctctgctgc
    20641 tatcccgaca tggaagccct catgcacggc gccgctgccc gggccaggcg cttgctgggc
    20701 ctggtctacc cgcgggatgg ctggtggacg aaggtaggcg tgacgcttct caacctctac
    20761 cctcgcctgc gtggtgatgc ctttcgttcc ttcgcccatc cgacggcggc cgtagaagct
    20821 gtggccgcct caaacgggct gcgccggatc acgcaccatg aggggctgat ttggcaggtg
    20881 gtggtgtttg cacgctgagg cctatatggc gacccaaggg atcatggctt cgggaacgat
    20941 gtgcctgtct gccccaattg ccatgccatc gttcaccggc gcacgccatg ttactccatt
    21001 gacgaggtga agggctttat tgcggccagc cagaacagac ttccggaacc ctcttagaat
    21061 atcctctgca tcaaagcgac ttagcgagac gacactctat aatcgggtgg atgcgccgca
    21121 ccctactgct tctcattgct tgcctgctat gtctgtccgc ctgccgccag gcgcctccac
    21181 cggagcagcc gacggctacc ccgctccctg taccgccggc tacagcggtc gatgggagtc
    21241 tcgccgccac atccgccccg acaggccaaa tcttcgtccc cgtcg
//
LOCUS       DAOSMQ010000003        20751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_2558_length_20751_cov_4.438152, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000003 DAOSMQ010000000
VERSION     DAOSMQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 20751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20751
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_20751_cov_4.438152"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..3235)
                     /locus_tag="PK829_00200"
     CDS             complement(20..3235)
                     /locus_tag="PK829_00200"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOU39667.1"
                     /translation="MTVLRTRITPPRPGPRTLERPRVTCLLAEALNYRLTLVQAGAGF
                     GKTTALAGLAALGRPLIWYQVMPEDRDPLVFLLHLCHASRLALPELSDLPISLLESWE
                     GTSSPLPSTHVLDEWLNSFAAAPVEPALLVLDDVHLVSSSPEIAGLLERLVNLAPAEL
                     HIVLAGRAPLNLPGLAGWTARGLVLAVERSALAFTAEEIATLFAQTYGYELTADEAAR
                     LFQATEGWAIALQLVWQSLRGGAFASVEEVLSNQASSLDSLFHLLATEIYAGQPADVQ
                     AFLQATSILRVITPEACDALGIGNSAVMLAYLRRIDLFLVDLGDGSLRYHHIFHSFLE
                     QLADPTARRMTHLLAAAYFQVAASDPGEALYHLQQAGDIGAMAALLDAYGAQLLASGR
                     LDTLAGHLANVPPEVLGGHPALLFYLGELARLRSRFQEALGWYQQAETAWREHGQMEG
                     VGRALRGQARVYLDTVNPSQAEALLEQSIRLSEGVEGHEMRARLYELLAENKLNAGHI
                     EAAESLRQQAEALRSASPSDSQLLSRVLLRTGRLDEAARRLEERAAAEQLAPVLTPRA
                     HRETHLLLSLVYALQGRGEDAYACAREGGRRGVDLRSPFVTAVGHMREGHALCLLPDA
                     GRREQARAQYETAIAISRELAVPRLIVEASWGLCRVYGYAGDLVRAEAAAAQGIDIAT
                     AAGDEWIASLVRVSMGAGLALAERYEAALEWLTRALQGFQECSDAFGACAARLWLATV
                     RRQQGKHDLLPQLLPAALAACEDNGYDYLLTRPTFLGPPDERVFTPLLILARARGWRG
                     EYAGRLLAALGLAAATHHPGYRVRVTTLGGFMAYKGDTAIASNTWRRAKARQLFQLLL
                     TYRRSGLDRDQILEYLWPGIEPAAAGRNFKVTLNTLYQVLEPHRQPGADSAFVHREGT
                     SYCLRTGADLWLDAERFAGLVAQARVARRQPPARAMALLEEAVALYKGDYLPEAIYET
                     WAAAERERLAVMFLGAADHLAELYLDNRRFEQAVEIAHRLLSADNCWERAYRHLMIAY
                     HRLGNRGQIARTYQRCVQTLREELDVAPTEETTALYAQLTD"
     gene            complement(3346..4311)
                     /locus_tag="PK829_00205"
     CDS             complement(3346..4311)
                     /locus_tag="PK829_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU39668.1"
                     /translation="MTFRRVMRLLGLLAGFAAGLTAAIVAVFTRRMIAPTRQPLWCAP
                     TDMGLAYENVDFPARDSVRLSGWFIPATPVAGGNPTVILVHGWGWNRLGDAAGDIVAD
                     LSGSTPVELLRLAHALHYDGYHVLMYDQRNHGESAAHPPVTFGQTEAEDLLGAIAYLQ
                     TRPEVDAARLGAIGFSMGANAILYALARTDNLRAAIAVQPMTMTTYSEGYAADLLGPL
                     AAVVQPLVATAYGAITGIPLRALQPAFAASGAAAGEDGRSLTPVLFVQCKADRWGSLA
                     DAERLAAATPASEGPLYVDGDHRYQGYQYLIDNPRIATTFLEQHL"
     gene            complement(4308..5204)
                     /locus_tag="PK829_00210"
     CDS             complement(4308..5204)
                     /locus_tag="PK829_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOU39669.1"
                     /translation="MIPSRRLLAVLAHPDDESFGIGGSLARYADEGVDVHIAIATDGV
                     AGSVAPGYEHTLVELAAVRRRELEEAIAVLGATLHTFGYRDSGYIGDPANQHPEAFIN
                     ADDVEATGRVVALIRQIRPQVVITHDETGGYYHPDHIHCWKITTAAFHMAGDPTQYPD
                     IGPAAYQPQRLYYTGFSNRIVKLLILMMRLRGQDPKHSGRNKDIDWTKIGLDPRQLNA
                     HIDYRRYWDVKRAAGAAHASQGGGMSRNRLLPEPFQRRFMATETYIRAYPPPREGERE
                     RDLFAGVVPDSTPTATQEDKGQ"
     gene            complement(5201..5728)
                     /gene="acpS"
                     /locus_tag="PK829_00215"
     CDS             complement(5201..5728)
                     /gene="acpS"
                     /locus_tag="PK829_00215"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOU39670.1"
                     /translation="MLATGIDLIEIDRVRRGLERYGTRFADRFFTRREQSQCAGRAAS
                     LAGRFAIKEAVGKALGTGIGDVGWKDIEVLNDDRGRPHLTLHNAAERLAAERGLSQWA
                     VSLSHTATHAVGLAVAAGDWPPRPLGEGHEDSPPRPLGEGHEGSPPRPLGEGHEDSPP
                     RPLGEGWGEGSEVIP"
     gene            complement(5910..7187)
                     /locus_tag="PK829_00220"
     CDS             complement(5910..7187)
                     /locus_tag="PK829_00220"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU39671.1"
                     /translation="MSAPSYPGPETIRRHVLDNGITVLLYENMSSNTVAIEGIVRAGA
                     VVEPAAQAGLADFTADLLIRGSSRWTFEASAEALESVGAGVSFGGSRHTTGFATHSLV
                     EDIDLVLDIAADALLRPLFPADHVERVRGQTITGLQMRANDTRDMARLAFYEAAYPGH
                     PYSRSVSGYEETVIGLARDDLASFHARTYGPRDMIIGLAGAIRADEALAKLNSAFGGW
                     RNDAQEVVPVVGDAARPVGLMRRVVAMPDKHQVDLLMGLPGPRRAAPDYLDASLMNSI
                     LGVFGMMGRIGQNVREKQGLAYYASSRLHGGLGPGPWVASAGVAPDKVEQAIASIRDE
                     IRRIQDEPVPAEELADSQAYRTGSLPMSLETNSGLVDVITDMELYNLGLDYLQTYTPR
                     ILAITADDVMAAARKYLSADDLVIAVAGPVDRE"
     gene            complement(7184..9001)
                     /locus_tag="PK829_00225"
     CDS             complement(7184..9001)
                     /locus_tag="PK829_00225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU39672.1"
                     /translation="MTAQSDLILDLQTLNQISQTLNQSTDVHTALNTSLAQLVDLMGM
                     ETGWVFVRAEAASDSWAGRGFRLAAHYNLPPALALTNPDAWDKSCDCQTLCREGQLTG
                     AYNEVRCSRLGSVDGLRNGLVVHATSPLRAGDRVLGILNVAAPAWSSFTPRSLTLLSN
                     VGSQMGIALERARLFDALQEQRIHEQATLLDLSQQLLSRRDLDDLMAFLVAEVRSLLS
                     ADACAVLLTGDEPDTLYFRAAAGWLSDPVGAGYRVPADDSTGSGRVMRSQSVLLIEDL
                     SRQDPPLWTPEWLAREGFRAAAIVPLVANGQSIGALVIDSRQPRQFSEVEVRLLRLMA
                     NQAALAIEKARLHHEELQRQRIEEELAVGRSIQRSMMPAQTPQLPGWQFATDFEAARQ
                     VGGDFYDYFPLPGEPGRWGLVIADVSDTGVPAALFMALSRTTIRNLALRGRAPADVIS
                     WANRFIQEDSQSDMFLTVFYAELDTRDGHLTYANAGHNPPLLWRSATSQFETLDTTCP
                     LLGVLPELDVRVVQAVIHPGDALVLYTDGITEAFDRDYNEFGRGRLEETIGDLLARRL
                     DATAADIQMAITAAVRDFIGSAVQPDDMTLLIIRRGETL"
     gene            complement(9032..10837)
                     /locus_tag="PK829_00230"
     CDS             complement(9032..10837)
                     /locus_tag="PK829_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU39673.1"
                     /translation="MKSFGQWLPASPLRPLARRFRNELPGSFDRNNRRLVIQSLVVGA
                     AVWAPVFVLKLLVHTTFVQVVAWLSGRPVVLVALPLLAGASIVALIVVRGRPAMIAYH
                     DDHDHIHELIDMEGDGVERAIALYYASEPSFERALLGKEGVEVRWELPTVSLAVRKFV
                     ATWATLGSGGSGGLEGSVALMGESIAAAIFKPRPLLRAGAQRRWLSAPLAWWSSVETD
                     HLQTAQLSGIAAAVATLTGAPFTSAFFAAEVMYRRRPLVEKLIYSLIATLVAYFLSSV
                     VHLGHQTLFTVDSLYLPSSSWGYYGLVLLISLIIVLVSAVFIQLRRALENAFHHRLPN
                     IWLRHLTGAALTALVSVVVVALTFGRGLIDHGGALQLVLGTGDWAIGRALAGELTLGL
                     ALVALVGKMVATATTIGSGGSAGLLIPSLYLGAMVATIVAVRTPYEPMTLIVPAMTAS
                     LVSIVNVPLAAMLLPVELFSGHYLPPALLAVIVCSLLTQKTPIYRSQREGFDNRQIAP
                     GVAVRRVMVPAHWDRRTLVDLDLRRRFNVTVIGVLELVGEGGRAQVRLDPAISLPLSI
                     GDTVVVMGADDDLDQLDRHIQMRMQETDIATSPSG"
     gene            complement(10834..12153)
                     /locus_tag="PK829_00235"
     CDS             complement(10834..12153)
                     /locus_tag="PK829_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU39674.1"
                     /translation="MALAVSKTQLDNGLTVVLKEMHHAPVATFMIWYRVGSRNEIPGR
                     TGVSHWVEHMMFKGTPQHPGALLDRLISREGGHWNAFTWLDYTAYYETMPADRIDIAL
                     RLEADRMVNTEMTPDEVESERTVILQERAMYANEPRFLLDEELTAAAFRVHPYHHEVI
                     GDTADLVSMTRDDLYGHYRRHYAPNNAIAVVVGDFDTTAMLARIAELFGGAEPGEAAA
                     PIHRAEPPQMGERRVTVHGPGDTAYLTFAYRAPAADHPDYPAMTLLNAAFAGGSSLGM
                     FAGGGSNRSSRLYKALVTTELAAGAYGGLIPTIDPFLYTINAVVRAGRAPAEVEAALE
                     SELARLATEPISAAELQKALKRARAQFIMAGESISGQAQLLGAAEATTGDYRWFETVL
                     ERLGQVTPEDIDRVRATYLTPRNRTVGLYEPDSRVATPDDEATETDE"
     gene            12321..13814
                     /locus_tag="PK829_00240"
     CDS             12321..13814
                     /locus_tag="PK829_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HOU39675.1"
                     /translation="MTTPDDPETAAKKDDKTPPVSPPVDVISQSSHAIMINGETVHYQ
                     ATAGRLILKAEDEKTGEKPKAAIFFVAYTRDRPTDASEAGYVAARPITFAFNGGPGSS
                     SVWLHLGLLGPRRVVLEEDGRPLPPPYRLVDNDLSLLDKTDLVFIDPVTTGFSRAVPG
                     EADKQFHGFKPDIESVGEFIRLYLSRNHRWASPRYLIGESYGTTRAAGLAGHLQERHG
                     LYLNGIMLISVVLNFMTLHFAPGNDLPYMLFLPTYAATAHYHGRLGPGVPAELPALLA
                     EVEAFAMNEYALALLRGDALPAEERAAIANRLAGYTGLSADYIARTNLRINIHRFTKE
                     LLRDRRRTTGRLDSRYTGIDRDAAGEYDEFDPSYALIQGPYTGLLNDYVRRELRFESD
                     LPYEILSDRVQPWSFDDHQNTYVNVGETLRQAMSMNPHLRVFVANGYYDLATPYLATR
                     YTFDHLALDESLRGNVTTTHYEAGHMMYIHDPSLAQLRADLVAFLAG"
     gene            complement(13811..14392)
                     /locus_tag="PK829_00245"
     CDS             complement(13811..14392)
                     /locus_tag="PK829_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOU39676.1"
                     /translation="MNCRLVHWFEPILLAVPCPGQKKNAMEKTLAMRALEGKRIPYEV
                     LTYPDDLRDAEEIALLLDLPAAEVFKTLVVLPPEPGKKPLLVMIPADTQLDLKKLAAA
                     VGAKKLKMATHHEAETLTGLQVGGISAVALLNKGFAVYIDRSSGGRDRSSGGRDRICV
                     SAGKRGLQLRLAAADLIKLTNARPVDVATREAA"
     gene            14470..15945
                     /locus_tag="PK829_00250"
     CDS             14470..15945
                     /locus_tag="PK829_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOU39677.1"
                     /translation="MNVVVATPDWVKDAIFYQIFPDRFARDPDDTSAGAAVMGTTFQP
                     WGSPPTVDSFQGGNLAGIAQHFDYLQQLGVNALYLNPIFASAANHRYIAHDYYQVDPI
                     LGGNAGFKRFLDAAHARGMRVVLDGVFNHCSRGLFQFHHLLEVGAESPYLDWFHVNKW
                     PLNAYTPGEHPNYAAWWNIASLPKFNTNNEGVREFLWGVGTYWLEQGIDGWRLDVPNE
                     IDDDEFWREFRRRCKAVNPDAYIVAELWKAAPRWLKGDQFDAQMNYLFTRAVLGFLVG
                     RDLDQTQTEPIGYGHVPRLDGAAFGREMERIINRLYHPEIAFAQLNMLGSHDTPRVMT
                     LANNQPDLVALAFLLQMTAPGAPNIYYGDEIGMDGRNDPYCRKAFPWHAPETWNTALL
                     DEVKRLTALRHRLAVLRRGSFCALQGVGRLAVYERRYGDERVVVAVNAARRAARFQLP
                     ADFGPALGEEPVGAALSRPLRGGELVEVPARSGRVWSSAAG"
     gene            16035..17036
                     /locus_tag="PK829_00255"
     CDS             16035..17036
                     /locus_tag="PK829_00255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU39678.1"
                     /translation="MATKLFVGDGVFEALAGEWDALATRGMTDTPFQTWAYQRAWWTH
                     LRPPDSTLLTVAARDEDDQLAAIGCFFHRDGALHFNGCIEETDYLDLIAPAERAEWAW
                     SAVLDCLETAEPAWTALALCNVPAASPTRAVLPALAQQHGYAVHEEQIEVCPTIPLPE
                     TFEAYLESLDSKQRRELSRKLRRAEGAELTTTAVGPAEDVHAAVERFLELLQMSTFEK
                     RDWLNDGRRAVFHEVARAAQTAGTLQLLFCGIEDRTAAALFNFDYRNRIWVYNSGLDP
                     SAFAALSPGVVLTAAAIERAVALGRAEFDFLRGDEEYKYRFGAVDQPIYRLHVSRAG"
     gene            17058..18401
                     /locus_tag="PK829_00260"
     CDS             17058..18401
                     /locus_tag="PK829_00260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU39679.1"
                     /translation="MTDLSALPDPSPTTYAGLRVAMLSVHTCPLAMLGGKKTGGMNVY
                     VRDLSRELGRMGLYVDVFTRSQDDCVPMVVHDLGEQARVIHIPAGPQAPIPVAEVGDY
                     LDEFAAGVVAFAAAEGLHYDIIHSHYWLSGLVAERLCAAWGETPVVHMYHTLGHMKNR
                     IAQGEAERAPQARIDGEAHVLDVADRIIAATPAEQAQLIWLYGADAAKVAIIPPGVDL
                     ARFHPIDKDEAKRCVGIPCGDNNIMFAGRIEPLKGIDTLIRAMALLKERFPTVVANTC
                     VAIIGGDPWADSPDAEMARLQALRQDLGIHDVVVFLGAKDQNVLPCYYAAAEVVVMPS
                     HYESFGMVALEAMAMGRPVIASEVGGLAFLIQDGVTGYHIPSRDPEALASRLYTLLAD
                     EGCRESMGRAARRYAEGFDWAIIAGRIVAVYADLLGRSALDASAFASLPLVDTTF"
     gene            18550..19059
                     /locus_tag="PK829_00265"
     CDS             18550..19059
                     /locus_tag="PK829_00265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOU39680.1"
                     /translation="MSLHFLILLAGLFCAAMAVRARPLTAALWLATLSAIIAIVLYRL
                     GAHEVAVIELSVGAGLITVLLAFAISMAEDETMPASIVPRPFAWLLVAVAGGALLWLL
                     LPSLPAARQLAADAPFIVQVWENRLFDLVLQMVALIAGVMGVLSLLGETRATGTTANE
                     PTPRMEGEQ"
     gene            19056..19361
                     /locus_tag="PK829_00270"
     CDS             19056..19361
                     /locus_tag="PK829_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOU39681.1"
                     /translation="MTPIQITIAGAILLVAVGLYGLLVSRHLIKVVIALQIMVKGAML
                     AIIVAGAAVGQMNLAQSMALTVIVVDTIAAVLALALIVQIKRRTGTLDAAELTQLRH"
     gene            19394..>20751
                     /locus_tag="PK829_00275"
     CDS             19394..>20751
                     /locus_tag="PK829_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOU39682.1"
                     /translation="MVPTLILIAIGAPWLGALVVWLLGDDRPRWQHGLASAAALAAAG
                     AALLLLPAGRWAATDEVALSLSFGPFIGDMTFVADSLAVFLAVIATTIGALTVIFSVG
                     YMASEAQLGRYYALVLAFIGAMCGLVLSGNLLFLFLFWEVTAFCSYALISFYNDDPRA
                     VAAGLKALIITQLGGVGLLVGVLMASAYLPDLQVSTLLASFGDLPAGTLAVVAFGFLL
                     AAMAKSAQAPLHIWLPDAMEAPTPVSALIHAATMVNAGVYLLARFYPAFAGVEGWTTA
                     VVIVGLASALLGGVLATTCNDLKRTLAYSTVSQLGFMVTGVGLGAIFESQFYLLSHAI
                     FKALLFLGAGAIIHAVGTRDMRQMGGLWGRMRVVSVCFLIGAAALMGLPFFNGFWSKE
                     ILLETALEQNPAVFLALLLGAALTAFYAVRMCWLVFFRQPETAAATSHAHADGHAQVS
                     PF"
BASE COUNT         3420 a         6911 c         6956 g         3464 t
ORIGIN      
        1 cctgagaccc gccccggccc taatcagtca gttgagcata cagggcggtc gtctcctcgg
       61 taggggccac atccagttcc tcgcgcaagg tctggacgca gcgctggtag gtgcgggcga
      121 tctggccgcg gttgccgagg cgatggtaag cgatcatcag gtggcggtag gcccgctccc
      181 aacagttgtc ggccgacagc agccggtggg cgatctcgac cgcctgctca aagcgccggt
      241 tgtcgaggta caactcggcc agatggtcgg ccgcgcccag gaacatgacc gccagccgct
      301 cgcgctcggc cgcggcccac gtttcataga tggcctccgg caggtaatcg cccttgtaga
      361 gagccacggc ctcttccagg agggccatcg cccgcgccgg gggttgccgc cgggcgaccc
      421 gcgcctgggc caccagcccg gcaaagcgct cggcgtccag ccacagatcg gcccccgtcc
      481 gcaggcaata gctcgtgccc tcgcggtgga cgaaggcgga gtcagcgccg ggctggcggt
      541 gaggctccag tacctgatag agggtattga gcgtcacctt gaaattgcgc ccggcggcgg
      601 ccggctctat gcccggccag agatactcca aaatctggtc gcggtcgagg ccagaccgcc
      661 ggtaagtcag gagcaattgg aagagctgcc gcgccttggc ccgccgccag gtgttggaag
      721 cgatggcggt gtcgcccttg taggccatga agccgcccag cgtggtcacg cgcacgcggt
      781 agccggggtg gtgcgtggcc gcggccaggc ccagcgccgc cagcagccgc ccggcgtact
      841 cgccgcgcca gccccgcgcg cgggccagga tgagcagcgg cgtgaagacg cgctcatcgg
      901 gcgggccgag aaaggtcggt cgcgtcagca ggtagtcgta gccgttatcc tcacaggcgg
      961 ccagggcagc gggcagcagt tgtggcaaga gatcgtgctt gccctgttgc cggcggacgg
     1021 tggccagcca caggcgcgcg gcgcaggccc caaaggcgtc gctgcattct tgaaagccct
     1081 gcaatgcccg cgtcagccac tccagcgcgg cctcatagcg ctccgccagg gccaggcccg
     1141 cgcccatcga tacgcgcacc agcgaggcga tccactcgtc gcccgcggcc gtggcgatgt
     1201 cgatgccctg cgccgccgcg gcttcggcgc gcaccaggtc gccggcgtag ccgtagacgc
     1261 ggcacaggcc ccaactggcc tcgacgatca gccgcggcac ggccaactcg cggctgatgg
     1321 cgatggccgt ttcgtactgg gcgcgggcct gctcgcggcg gcccgcgtcg ggcagcaggc
     1381 acagggcgtg gccctcgcgc atgtggccga cggcggtcac gaacggcgag cgcagatcga
     1441 cgccacggcg tccgccctcg cgggcgcagg cgtaggcgtc ctcgccccgc ccctgcaggg
     1501 catagaccag cgacaacagc aggtgcgtct cgcggtgggc gcggggtgtc aataccgggg
     1561 ccagttgctc ggccgcggcg cgctcctcca gccggcgggc ggcctcgtcc agccggccag
     1621 tgcgcagcag cacgcgggag agcaactgcg aatcggacgg gctggcgctg cgcagggcct
     1681 cggcctgctg tcgcaggctc tcggctgcct cgatgtggcc ggcgttgagc ttgttctcag
     1741 ccagcagttc gtaaagccgg gcgcgcatct cgtgcccctc gaccccttca ctcaggcgga
     1801 tcgactgttc cagcagtgcc tcggcctggc tggggttgac cgtgtcgaga tagacacgtg
     1861 cctggccgcg cagcgcccgg ccgacgccct ccatctggcc atgctcgcgc caggctgtct
     1921 cggcctgttg gtaccagccc agagcctcct ggaagcggct gcgtagccgg gccaactcgc
     1981 ccaggtagaa cagcagtgcc gggtggccgc ccagcacctc tggcggcacg ttggccagat
     2041 ggccggccag cgtgtccagc cgccctgagg ccagcaattg cgcgccatag gcatccagca
     2101 gcgcggccat cgcgccaata tcacccgcct gttgcagatg atagagcgcc tcgccggggt
     2161 cgctggccgc cacctggaag taggccgcgg ccagcagatg ggtcatgcgc cgagcggtgg
     2221 ggtcggccag ttgctccaga aagctgtgga agatgtggtg atagcgtagg ctgccgtcgc
     2281 ccagatcgac gaggaacagg tcgatgcgcc gcagataggc cagcatgacg gcgctgttgc
     2341 cgatgcccag cgcgtcgcac gcctccggcg tgatgacgcg caggatggag gtcgcttgca
     2401 ggaaggcctg cacatcggcc ggctgcccgg catagatctc cgtggccagc aggtggaaga
     2461 gactgtcgag tgacgacgcc tgattagaca gcacctcttc gacagaagcg aacgcgccgc
     2521 cgcggaggct ttgccagacg agttgcaggg caatggccca accctccgtc gcctggaaga
     2581 gccgcgccgc ctcgtcggcg gtcagctcat agccgtaggt ctgcgcgaag agggtggcaa
     2641 tttcctcggc ggtgaaggcc agcgccgagc gctcaaccgc tagcaccagc ccgcgcgctg
     2701 tccagccggc caggccgggc aggttcagcg gcgcgcgccc tgccaggacg atgtgaagct
     2761 cagctggggc caggttcacc agccgctcca acagcccggc gatctccggc gacgacgaaa
     2821 cgagatggac gtcgtcgagc accagcagcg ccggttcgac gggcgcggcg gcgaagctat
     2881 tgagccactc gtcaaggacg tgggtcgagg gcagtgggct gctggtgccc tcccatgact
     2941 ccagtagcga gatgggcagg tcgctcagtt ccggcagggc cagacgggag gcgtggcaga
     3001 ggtgtagcaa gaagaccagc ggatcgcggt cttcgggcat gacctgatac cagatcagcg
     3061 gccggcccag ggcggccaac cccgccagcg ccgtcgtctt gccgaagccc gccccggcct
     3121 gcaccagcgt cagccggtag ttcagcgcct cggccagcag acaggtcacg cgcggccgct
     3181 ccagcgtgcg cgggccaggc cgcggcggcg tgatcctcgt gcgtagaacg gtcaccggcc
     3241 tgagcattgt ccgccccaat agggaccagg ttccaggttc caggttccag gcgctaggga
     3301 agaacgctct tactagcctc tggaacctgg cccctggccc ctagctcaga gatgctgctc
     3361 caagaacgtc gtggcgatgc gtgggttgtc gatgaggtac tgatagccct gatagcgatg
     3421 gtcgccatcg acatagagcg gcccctcgct ggcgggcgtg gccgcggcca gccgttcggc
     3481 atcggccagc gaaccccagc ggtcggcctt gcactgcacg aagagcaccg gcgtcagcga
     3541 gcggccgtcc tcgccggcgg ccgcgccgct ggcggcaaag gccggttgca gcgcccgcag
     3601 ggggataccg gtaatggccc cgtaggctgt cgccaccaac ggctgcacca ccgcggccag
     3661 cggccccagc agatcggcgg cgtagccctc actgtaggtg gtcatcgtca tcggctggac
     3721 agcgatggcc gcccgcaagt tatcggtgcg agccagcgca tagagaatgg cgttcgcgcc
     3781 catcgagaag ccaatggccc ccaggcgcgc cgcgtccacc tccggccgcg tttgcaagta
     3841 ggcgatagcc cccagcaagt cctcggcctc ggtctggcca aaggtgaccg gcgggtgggc
     3901 ggcgctctcg ccgtggttgc gctggtcgta catcagcaca tggtagccgt cgtaatgcag
     3961 cgcgtgggcc aaccgcagca gctccaccgg cgtcgaaccg ctcagatcag cgacgatgtc
     4021 gccggcggcg tcgcccagcc ggttccagcc ccaaccgtgg acgaggatga cggtggggtt
     4081 gccgccggcg accggggtcg ccgggatgaa ccagcccgac aggcgtacac tatcgcgcgc
     4141 gggaaagtca acgttttcat aggccaggcc catgtcggtg ggcgcgcacc agagcggctg
     4201 gcgcgtcggg gcgatcatgc ggcgggtgaa gacggccacg atggccgccg tcagcccggc
     4261 cgcaaagccg gccaacaggc cgagaagtcg catcacacgt cggaatgtca ttgtcccttg
     4321 tcctcctggg tggccgtagg ggtgctgtcg ggcacgacgc cggcaaacag gtctcgctcg
     4381 cgttcgccct ctcttggcgg tgggtaggcg cggatgtagg tctccgtagc catgaagcga
     4441 cgctggaacg gctccggcag cagccggttg cgcgacatgc cgccgccctg gctggcgtgg
     4501 gccgccccgg cggcgcgctt cacatcccag tagcgtcggt agtcgatgtg ggcgttcagc
     4561 tgccgcgggt cgaggccgat cttcgtccag tcaatgtcct tgttgcggcc ggagtgcttg
     4621 gggtcttgcc cgcgcaggcg catcatcaag atgagtagct tgacgatccg gttggaaaaa
     4681 ccggtgtagt agaggcgctg gggctgatag gctgccgggc cgatgtcggg atactgcgtc
     4741 gggtcgccgg ccatgtgaaa ggcggccgtc gtgatcttcc agcagtggat gtggtcgggg
     4801 tggtaatagc cgcccgtctc gtcgtgggta atgaccacct gtggacgaat ctggcggatg
     4861 agggcgacga cgcggccggt ggcctccaca tcgtcggcat tgatgaaggc ttccgggtgc
     4921 tggttggccg ggtcgccgat gtagccggag tcgcggtagc caaaggtgtg cagcgtcgcc
     4981 cccaggacgg cgatggcctc ctccaattcg cgacggcgga cggcggccaa ctcgaccagg
     5041 gtatgttcgt agccgggggc cacggagccg gccacgccgt cagtggcgat ggcgatgtgg
     5101 acgtcgacgc cctcgtcggc atagcgggcc aacgagccgc caatgccgaa ggattcgtcg
     5161 tcggggtggg ccaggacggc cagaaggcga cgggagggaa tcatggaatc acctcactgc
     5221 cctctcccca accctctccc agagggcgag ggggcgaatc ttcatggccc tcccccaaag
     5281 gacgaggggg cgaaccttca tggccctctc ccagagggcg agggggcgaa tcttcatggc
     5341 cctctcccag agggcgaggg ggccaatccc ccgcagccac ggccaggccg acggcgtggg
     5401 tggccgtgtg cgagaggcta acggcccact ggctcaggcc gcgctcggcg gccagccgct
     5461 cggcagcgtt gtggagtgtc aggtggggcc ggccgcggtc gtcgttgagc acctcgatgt
     5521 ccttccagcc gacgtcgccg atgcccgtgc ccagcgcctt gccgaccgct tctttgatgg
     5581 cgaaacggcc ggccaggctg gcggcgcgcc cggcacactg gctctgctcg cgccgggtga
     5641 agaagcgatc ggcgaagcgc gtgccgtagc gctccagccc gcgacgcacg cgatcgattt
     5701 cgataaggtc aatgcctgta gccaacatta ctgccctgcg cctccaagta ggggctaggt
     5761 tctaggggct agagattggg aagagcgttc ttctcatgga acctggcctc tgtttcgggt
     5821 tctaagaagt tctaagttcc aggaaagaag cgttcttctc ctagccccta acccctattc
     5881 cctattccct agtccctagt ccctagtccc tattccctat ccaccggccc cgcgaccgca
     5941 atgaccaggt catcagcgct gaggtacttg cgtgccgcag ccatgacgtc atcggctgtg
     6001 atggccagaa tacggggcgt gtaggtctgc aagtaatcca gacccaggtt atatagctcc
     6061 atgtcggtga tgacatcaac caggccgctg ttggtctcca ggctcatggg cagcgacccg
     6121 gtacggtagg cctggctgtc ggccagctcc tcggccggca ccggctcgtc ctggatgcgg
     6181 cgaatctcgt cgcggatgga ggcgatggcc tgctccacct tgtctggggc caccccggcc
     6241 gaggccaccc acggccccgg ccccagcccg ccgtgcagcc ggctggaggc gtaataggcc
     6301 aggccctgct tctcgcgcac gttctggccg atgcgcccca tcatgccaaa gacgcccagg
     6361 atggagttca tcaggctggc gtcgagatag tcaggcgcgg cgcgacgcgg gccgggcaga
     6421 cccataagca aatcgacctg atgcttgtcg ggcatggcca ccacccggcg catcaggccg
     6481 accggccggg ccgcgtcgcc caccacgggc acaacctcct gcgcgtcgtt gcgccagccg
     6541 ccgaaagcgc tattgagctt ggccagcgct tcgtccgcgc gaatggcccc ggccagaccg
     6601 atgatcatgt ctcgcgggcc ataggtacgg gcgtggaagc tggccaggtc gtcgcgggcc
     6661 agaccaataa ccgtctcctc gtagccgctg accgagcggc tgtaggggtg gccggggtaa
     6721 gccgcctcat agaaggccag ccgggccatg tcgcgggtgt cgttggcccg catctgcaag
     6781 ccagtgatgg tctggccgcg gacgcgctcg acgtgatcgg ccgggaagag cggccgcagc
     6841 agcgcgtccg cggcaatgtc gagcaccagg tcgatgtctt cgaccagact gtgggtggcg
     6901 aagccggtcg tgtgacggct gccgccgaag gacacacccg cgccaactga ctccagcgcc
     6961 tcggcactgg cctcgaacgt ccagcgcgaa ctgccgcgaa tgagcaggtc ggcggtgaag
     7021 tcggccagtc cagcctgcgc ggccggctcc accaccgccc ccgcgcggac gatgccctct
     7081 atggcgacag tattactgga catattctcg tagagcagca cggtgatgcc gttgtcgagg
     7141 acgtggcggc ggattgtctc cgggccggga taactgggag cgctcataag gtctctccgc
     7201 gtcggatgat cagaagggtc atgtcgtccg gctgcaccgc gctgccgatg aagtcgcgca
     7261 cggccgcggt gatggccatt tggatgtcgg cggccgtggc gtcgaggcgt cgcgccaaca
     7321 agtcgccgat agtttcttca aggcggccgc gaccaaactc gttgtagtcg cggtcgaagg
     7381 cctcggtgat gccgtcggtg tagagcacca gcgcgtcacc aggatggatg accgcctgga
     7441 caacgcggac gtctagctcc ggcagcacgc ccagcagcgg gcaggtcgtg tccagcgtct
     7501 cgaactggct ggtggctgat cgccacagca ggggcgggtt gtgaccagcg ttggcgtagg
     7561 tcaggtggcc gtcgcgcgtg tctagctcgg cgtagaagac ggttagaaac atgtccgact
     7621 ggctgtcttc ctggatgaaa cggttggccc aggagatgac atcggctggg gcgcggccgc
     7681 gcagggccag gttgcggatg gtcgtgcggc tgagggccat gaacagcgcc gcgggcacgc
     7741 cggtgtcgga cacgtcggcg atgaccagcc cccagcgccc cggttcgccc ggcaggggaa
     7801 agtagtcgta gaagtcgccg ccgacctggc gagcagcctc gaagtcagtg gcgaactgcc
     7861 agccaggcaa ctgcggcgtc tgggccggca tcatgctgcg ctggatgctg cggccgacgg
     7921 ccagctcttc ctcaatgcgc tgtcgctgca actcctcgtg gtgcaggcga gccttttcga
     7981 tggccagcgc cgcctgattg gccatcagtc gcaacaggcg cacctcgacc tcgctgaact
     8041 gccgcggctg gcgcgagtcg atgaccagcg cgccaatcga ctgcccattg gctaccaggg
     8101 gcacgatggc cgccgcccgg aagccttcgc gggccagcca ttctggcgtc cacaagggcg
     8161 ggtcctggcg cgacaggtcc tcgatcagca ggacgctctg gctgcgcatg acccggccag
     8221 agccggtcga gtcgtcggcg ggcacgcgat agcccgcgcc aacgggatcg ctcaaccagc
     8281 cggcggcggc ccggaagtag agcgtatccg gctcgtcgcc tgtcagcagc accgcgcagg
     8341 cgtcggccga caacaggctg cgcacttcag cgacgaggaa agccatgagg tcatcgagat
     8401 cgcggcggct gaggagttgc tgcgagaggt ctagcagcgt ggcctgctcg tggatgcgct
     8461 gttcttgcag ggcgtcgaac aggcgcgccc gctccagggc gatgcccatc tggctgccga
     8521 cgttgctgag cagcgtcagg ctgcgcggcg tgaaggacga ccaggccggc gcggcgacat
     8581 tgaggatgcc caacacgcgg tcgccggcgc gcagaggtga cgtcgcgtgg acgaccaggc
     8641 cattgcgcag accgtcaacg ctgcccagcc ggctgcaacg cacttcgttg taggccccag
     8701 tcagctgtcc ctcgcggcaa agggtctggc agtcgcagct cttatcccag gcgtcggggt
     8761 tcgtcagggc cagcgcaggc ggcaggttat agtgcgccgc caggcggaag ccgcggccgg
     8821 cccagctgtc gctagcagcc tccgcgcgca cgaacaccca acccgtctcc atgcccatca
     8881 gatcgaccag ttgcgccagc gacgtgttca gcgccgtgtg gacatcggtt gactgattca
     8941 gggtctggga gatctgattc agcgtttgca ggtcgaggat cagatcggac tgcgcggtca
     9001 tttcgtccaa ttctaacata gtcctcgcgg cctagccgct gggcgacgtg gctatatcag
     9061 tctcctgcat gcgcatctgg atatggcgat ccagttgatc caggtcgtcg tcggccccca
     9121 tgacaacaac agtatcgcca atgctgagcg gcaacgagat ggccgggtcg agtcgcacct
     9181 gggcgcggcc gccttcgccg acaagctcga gcacgccgat gacagtgacg ttgaagcggc
     9241 ggcgcagatc gagatcgacc agcgtgcgcc gatcccaatg ggccggaacc atcacccgcc
     9301 gcacggccac gccgggcgca atctgccggt tgtcgaagcc ctcgcgctgg gagcggtaga
     9361 tgggcgtctt ctgcgtcagc aaggagcaga cgatcacggc cagcaacgcc ggtggcaggt
     9421 agtggccgct gaacagctcc acgggcagca gcatggcggc gaggggcacg ttgacgatgg
     9481 acaccaggct ggccgtcatc gccggaacga tgagcgtcat cggctcgtag ggcgtcctga
     9541 cggcgacaat ggtggccacc attgcaccca gatagagtga cggaatgagc agcccggccg
     9601 agccgccgga gccgatggtc gtggccgtgg ccaccatctt gccgaccagg gccactagcg
     9661 ccagccccag ggtcagttcg ccggccagcg cgcgcccgat cgcccagtcg cccgtcccca
     9721 gcaccaattg cagcgccccg ccgtgatcga tgagtccccg gccaaacgtg agggccacca
     9781 caacgacgct caccagggcc gtcagggccg cgccggtcag atgtcgcagc cagatattgg
     9841 gcagacggtg atggaatgcg ttttccaggg cgcgccgcaa ctggatgaag acggcgctga
     9901 ccaggacgat gatcagcgag atgagcagaa ccagtccata gtagccccaa ctgctggacg
     9961 gcagatagag cgaatcgacg gtaaaaagcg tctggtggcc aaggtggaca acgctgctga
    10021 ggaagtaagc caccagtgtg gcgatgagcg aatagatgag cttctccacc aacggccggc
    10081 ggcggtacat gacctcggcg gcgaaaaagg ccgacgtgaa cggcgcgccg gtcagcgtgg
    10141 ccacggccgc ggcgatgccg ctcagttgag ccgtctgcaa gtgatccgtc tccaccgagg
    10201 accaccacgc cagcggggcg ctgagccacc ggcgctgggc cccggcgcgc agcagggggc
    10261 gaggcttgaa tatggccgcg gcgatgcttt cgcccatcag ggcgacgctg ccctccaggc
    10321 cgccactgcc gccgctgccc agtgtcgccc acgttgccac gaacttgcgc acggccagcg
    10381 acacggttgg cagctcccag cgtacctcga cgccctcttt gcccagcagc gcccgctcaa
    10441 acgacggctc ggaggcgtag tacagggcga tggcccgctc cacaccatcg ccttccatgt
    10501 cgatcagttc atggatgtgg tcgtggtcat cgtgataggc gatcatcgcc gggcggccgc
    10561 ggacgacaat gagggcgacg atgctcgccc cggccagcag cggcaaggcc accagaacca
    10621 ccggccgccc gctcagccag gccacgacct gtacaaacgt cgtatgaacc aacaacttga
    10681 gcacgaagac cggcgcccag acggccgcgc caacgacgag cgactggatg accagtcgcc
    10741 ggttattacg gtcgaagctg cccggcagtt cgttgcggaa gcgacgcgcg aggggccgca
    10801 aggggctggc gggcaaccac tggccgaacg acttcattcg tctgtctcgg tcgcctcgtc
    10861 gtctggcgtg gccacgcggc tatctggctc gtagaggccg acggtgcggt tgcgcggcgt
    10921 cagataggtg gcccggacgc ggtcgatgtc ctccggtgtc acctggccca ggcgctccaa
    10981 gaccgtctcg aaccagcgat aatcgcccgt cgttgcctcg gccgcgccca atagttgggc
    11041 ctggccggag atgctttcgc cggccatgat gaactgtgcc cgcgcccgct tgagggcctt
    11101 ttgcagctcg gcggccgaga tcggctccgt cgccaggcgg gccagttcgc tctccagggc
    11161 cgcttcgacc tcggccggcg cgcgcccggc gcgaacgacg gcgttgatgg tatagagaaa
    11221 ggggtcgatt gtcggaatca ggccaccata agcgccagcg gccagttctg tcgtcaccag
    11281 agccttgtag aggcgcgaac tgcgattgga gccacccccg gcgaacatgc ccaggctgct
    11341 gccgccggca aaggcggcgt tgagcagcgt catggccggg tagtcggggt ggtcggccgc
    11401 cggcgcgcga taggcgaagg tcagataggc ggtgtcgccg gggccgtgaa ccgtcacccg
    11461 acgctcgccc atctggggcg gctcggcgcg gtggatgggc gcggccgcct cgcccggctc
    11521 agcgccgcca aatagctcgg cgatccgcgc cagcattgct gtggtgtcaa agtcgccgac
    11581 gacaacagcg atggcgttat tgggcgcata gtggcggcgg tagtggccgt agaggtcgtc
    11641 acgggtcatg ctgaccagat cggccgtgtc gccgatgacc tcatgatgat aggggtggac
    11701 gcggaaggcc gccgccgtca gttcctcgtc caacaagaag cgcggctcgt tggcatacat
    11761 agcccgctct tgcaggatga ccgtccgctc cgattcgacc tcgtccgggg tcatctccgt
    11821 gttgaccatg cggtcggcct ccaggcgcag ggcgatgtcg atgcggtcgg cgggcatcgt
    11881 ttcgtagtaa gcggtgtagt cgagccaggt gaaggcgttc cagtgaccgc cctcacggga
    11941 aatcaggcgg tcgagcagag cgccagggtg ctggggcgtg cccttgaaca tcatatgttc
    12001 gacccagtgg gatacgcccg tgcggccggg aatttcgttg cggctgccga cgcgatacca
    12061 gatcatgaag gtcgccacag gggcgtgatg catctctttg aggacaacgg tcaggccgtt
    12121 gtcgagttgt gtcttgctaa ctgccagtgc catccactta ctccggcgaa acgaattaat
    12181 cgacccaagc tcgtgattat agcgcgggcg gccgatggcg acaggaaata ctcggcgcgg
    12241 gcacggctgc gtccgaatct ggccacgctg gccaagttgt gcttgaatgg ggccatcacc
    12301 cccacccaag gagaccgccc gtgaccacgc ccgatgaccc agagacagcc gccaagaaag
    12361 acgacaagac cccacccgtt tcgccgcccg tagatgtcat ctcccagagc agccacgcga
    12421 tcatgatcaa cggcgagacc gttcactacc aggctactgc cggccgtctc atcctcaagg
    12481 ccgaggacga gaagacgggc gagaaaccca aggcggctat cttcttcgtg gcttacacac
    12541 gcgatcgtcc gacggacgcg agcgaggcag gctacgtcgc cgcccggccg atcaccttcg
    12601 ccttcaacgg cggccccggc tcgtcgtcgg tgtggcttca tctggggctg ctcggcccgc
    12661 ggcgcgtcgt gctggaggag gacggccggc cgctgccgcc gccctatcgc ctggtggaca
    12721 acgacctcag cctgctggac aaaaccgacc tggtgttcat cgacccggta acgaccggct
    12781 tcagccgcgc cgtgcccggc gaagcggaca agcagttcca cggcttcaag cccgacatcg
    12841 agtctgtggg cgagttcatc cgcctttacc tgtcgcgcaa ccaccgctgg gcctcgccgc
    12901 gctacctcat cggcgagagc tacggcacga cccgcgccgc cggcctggcc ggccacttgc
    12961 aggagcgtca cgggctgtac ctcaacggca tcatgctcat ttccgtggtg ctgaacttca
    13021 tgaccctcca cttcgcgccg ggcaacgacc tgccctacat gctcttcctg cccacctatg
    13081 cggccacggc ccactaccac ggccgcctgg ggccgggcgt gcccgcggag ttgccggcgc
    13141 tgttggccga ggtcgaggcg ttcgccatga atgaatatgc cctggcgctg ctgcgcggcg
    13201 acgcactgcc ggccgaggag cgcgccgcca tcgccaacag gttggcgggc tacaccggcc
    13261 tgtcggccga ctacatcgcc cgcaccaacc tgcgcatcaa catccaccgc ttcacgaagg
    13321 agctattgcg cgaccggcgg cgcacaaccg gccgcctcga cagccgctac acgggcatcg
    13381 accgcgacgc ggccggcgag tacgacgagt ttgaccccag ctacgccctc atccaggggc
    13441 catacaccgg cctgctcaat gactacgtgc ggcgcgaact gcgcttcgag agcgatttgc
    13501 cctacgagat tctcagcgac cgggtgcagc cgtggtcatt cgacgaccac cagaacacct
    13561 acgtcaacgt gggcgaaacg ctgcggcagg ccatgagcat gaacccgcac ctgcgcgtgt
    13621 tcgttgccaa cggctactac gacctggcga cgccctacct ggcgacgcgc tacacattcg
    13681 accatctggc actggacgag agcctgcgcg gcaacgtgac cacgacccac tacgaagccg
    13741 ggcacatgat gtacatccac gatccgtcgc tggcccaact gcgggccgac ctggtggcgt
    13801 ttctggcggg ctaagcggcc tcacgggtag ccacgtccac cggccgggcg ttggtcagct
    13861 tgatgaggtc agccgcagcc aggcgcaatt gcaagccgcg cttacccgcg ctgacacaga
    13921 tacggtcgcg cccgccggac gagcggtcgc gcccgccaga cgagcggtcg atgtagacgg
    13981 cgaagccctt gttgagcagg gccacagcgg agatgccacc gacctgtaga ccggtcagcg
    14041 tctccgcctc gtggtgggtg gccatcttga gcttcttggc tcccacggcg gcggccagct
    14101 tcttcaggtc gagttgcgta tcggccggaa tcatcaccag cagcggcttc ttgcccggct
    14161 ccggcggcag aacgaccagc gttttgaaga cctccgccgc gggcaggtcg aggaggaggg
    14221 cgatctcctc ggcgtcgcgg aggtcgtcgg gataggtcag gacttcatag ggaatgcgct
    14281 tgccctccag ggcgcgcatg gccagcgttt tctccatcgc gtttttcttc tggccggggc
    14341 agggcacggc caatagtatt ggctcgaacc agtggacaag tctacaattc acttcgcgcg
    14401 gcggtctctg ctcaacgagt gtgctcgcgc cgaactgagc cattatacca ccggccggaa
    14461 ggaaccaaga tgaacgttgt ggttgcgacg cccgactggg tcaaggatgc tatcttctac
    14521 cagattttcc ccgatcgttt cgcccgcgac cccgacgaca ccagcgccgg ggcggcggtc
    14581 atggggacga cgtttcagcc gtggggcagc ccgccaacgg tcgatagctt ccagggtggc
    14641 aacctggccg gcatcgccca gcacttcgac tatctgcaac aactgggcgt caacgcgctc
    14701 tacctgaacc ccatcttcgc ctcggcggcc aaccaccgct acatcgccca cgactattac
    14761 caggtcgatc ccattctggg cggcaacgcc ggcttcaagc gctttctaga cgcggcccat
    14821 gcccgcggca tgcgcgtggt gctcgacggc gtcttcaacc attgcagccg cggcctgttc
    14881 cagttccacc atctgctgga agtcggcgcg gagtcgccct accttgactg gttccacgtc
    14941 aacaagtggc cgttgaacgc ctacacgccc ggcgaacatc ccaactacgc cgcctggtgg
    15001 aacatcgcct cgctgcccaa gttcaacacc aacaacgagg gcgtgcgcga gttcctgtgg
    15061 ggcgtgggca cctactggct ggagcagggc atcgacggct ggcggctgga cgtgcccaac
    15121 gagatcgacg acgacgagtt ctggcgcgag ttccgccgcc gctgcaaggc cgtcaatccc
    15181 gacgcctata tcgtcgccga gctgtggaag gcggccccac gctggctgaa gggcgaccag
    15241 ttcgacgcgc agatgaacta tctgttcacg cgggccgtgc tgggcttcct cgtcggccgc
    15301 gacctggacc agacgcagac ggagccaatc ggctatggtc acgtgccccg gcttgacggc
    15361 gcggcgttcg gacgcgaaat ggagcgcatc atcaaccggc tctaccaccc ggagatcgcc
    15421 ttcgcccaac tcaacatgct tggcagccac gacacgccgc gcgtcatgac actggccaac
    15481 aatcagcccg acctggtggc cctggccttc ctcttacaga tgaccgcgcc cggcgcgccc
    15541 aacatctact acggcgacga gatcggcatg gacggccgca acgaccccta ctgccgcaag
    15601 gccttcccct ggcacgcgcc agagacgtgg aacacggcct tgctggacga ggtcaaacgg
    15661 ctgacggccc tgcgccaccg gctcgcagtc ctgcgccgcg gcagcttctg cgccctgcag
    15721 ggcgtcgggc ggttggccgt ctatgagcgg cgctatggtg atgagcgcgt cgtcgtcgcc
    15781 gtcaacgcgg cccggcgggc ggcgcgcttc cagttgccgg cagacttcgg cccggcgctg
    15841 ggcgaggagc cggtcggcgc ggcgctgagc cggccgctgc gcggcggcga gttggtggag
    15901 gtgccggccc gcagcgggcg cgtctggtcg tcggccgccg ggtaaactgc ggcttttatt
    15961 ctgatacact tcctatatcg tggcccgcgg cggcgggcta taatgacagg tgctaaccga
    16021 gaatgaggat ggttatggca acgaagctgt ttgtgggcga cggcgtcttt gaagccctgg
    16081 cgggcgagtg ggacgcgctg gcgacgcgcg gcatgaccga cacgccgttt cagacgtggg
    16141 cctatcagcg cgcctggtgg acgcacctgc ggccgcccga cagcacgctg ctcacggttg
    16201 ccgcccgcga cgaggatgac cagttggcgg ccatcggctg ctttttccat cgggacgggg
    16261 cgctacactt caacggctgt atcgaggaaa ccgactacct cgacctgatc gccccggccg
    16321 agcgggccga gtgggcctgg agcgccgtgc tggactgtct ggaaacagcc gagccagcct
    16381 ggacggccct ggccctgtgc aacgtccccg ccgcctcacc gacgcgggcc gttttgccag
    16441 cgctggccca gcagcacggc tatgccgtcc acgaggagca gatcgaggtc tgcccgacca
    16501 ttcctctgcc ggagacgttt gaggcttacc tggagagcct cgacagcaag cagcgccgcg
    16561 aactcagccg caagctgcgt cgggccgagg gtgccgagct gaccacgacc gccgttggcc
    16621 cagccgaaga cgtgcacgcc gccgtggaac gcttcctgga actcttgcag atgagcacct
    16681 tcgagaagcg cgactggctc aatgacggcc gccgggcggt ctttcacgaa gtggcgcgtg
    16741 ccgcccagac ggccggcacg ctgcaactgc tattctgcgg cattgaggac cggacggcgg
    16801 cggcgctgtt caacttcgat taccgcaacc gcatctgggt ctacaactcc ggcctcgacc
    16861 ccagcgcttt cgccgcgctc agccccggcg tcgtgctgac cgccgcggcc atcgaacgcg
    16921 ctgtagccct cggccgggct gagtttgact tcctgcgcgg cgacgaggaa tacaagtacc
    16981 gttttggggc cgtcgaccag cccatctatc gtctgcacgt cagccgggcc ggctgatcgg
    17041 gtatgaaagt gaccgcgatg actgaccttt ccgcccttcc tgatccctcc ccgacgacct
    17101 acgccgggct gcgcgtggcc atgctcagcg tccacacttg cccgctggcc atgctgggcg
    17161 gcaagaagac gggcggcatg aatgtctacg ttcgcgacct gagccgcgaa ttgggccgca
    17221 tgggcctcta cgtcgatgtg ttcacgcgct cacaggacga ttgtgtgccc atggtcgtcc
    17281 acgatctggg cgaacaggcg cgggtgatcc acattcccgc cgggccacaa gcgcccattc
    17341 ctgtggccga ggtgggcgac tatctcgacg agttcgccgc cggcgtggtc gccttcgccg
    17401 cggccgaagg cttgcattac gacatcatcc acagccacta ctggctctcc gggttggtgg
    17461 ccgagaggct ttgcgccgcc tggggcgaga cgccagtggt gcacatgtac cataccctgg
    17521 gacacatgaa gaaccgcatc gcccagggcg aggccgaacg cgccccgcag gcgcgcatcg
    17581 acggcgaggc gcacgtgctg gatgtggccg accgcatcat cgccgcgacg ccggctgagc
    17641 aggcccaact catctggctc tatggggccg acgccgccaa ggtggccatc atcccccccg
    17701 gggttgacct ggcccgtttc catcccatcg acaaggatga ggccaagcgc tgtgtaggca
    17761 tcccctgcgg cgataacaac atcatgtttg ccggacgtat cgagccgctg aagggcatcg
    17821 acacgctcat ccgcgcgatg gccctgctca aggagcgctt tcccacggtg gtcgcgaaca
    17881 cctgcgtggc gatcatcggc ggcgacccgt gggccgactc gcccgacgcg gagatggccc
    17941 ggctgcaagc cctgcggcaa gacctgggca tccatgatgt cgtcgtcttt ctgggggcca
    18001 aagaccagaa cgtgctgccc tgctactacg ccgctgccga ggtcgtggtc atgccctccc
    18061 actacgagag cttcggcatg gtcgccctgg aggcgatggc catgggccgg ccggtgatcg
    18121 cttcggaagt gggcggcctg gccttcttga tccaggacgg tgtgaccggc taccatatcc
    18181 cttcgcgcga tccggaggcg ctggccagtc gtctctatac gctgcttgcc gatgagggct
    18241 gtcgtgagag tatgggccgg gcggcgcggc gctacgctga gggcttcgac tgggccatca
    18301 tcgctgggcg cattgtcgcg gtctacgccg acctcctcgg gcgcagcgct ctcgatgcca
    18361 gtgcgtttgc ctctctaccc ctcgtcgata ccacttttta gccccctttt agatagattg
    18421 cccaaaagaa tcgggtttgt ttgtgacagt tttcacaaat caggatgttt gtcatgcctt
    18481 tgacatgaca agtatcactt atcacgtgcc tgcctctgtc ctattgttct agtatgagga
    18541 ggtgacgtga tgtcgcttca ctttctgatc cttcttgcgg gcctgttctg cgcggcgatg
    18601 gccgtgcgcg cccgccccct gacagcggcg ctgtggctgg ccacgctcag cgcgatcatc
    18661 gccattgtgc tctatcgcct cggcgcgcac gaggttgccg tcattgagct gagcgttggc
    18721 gccggcctca tcactgtgct gctggccttt gccatcagca tggctgagga cgaaacaatg
    18781 cccgcgtcga ttgtgccccg gccgtttgcc tggctgctgg tggccgtagc cggcggcgcg
    18841 ttgctgtggc tgctgctgcc ctccctgccg gcggcgcgcc agctagccgc cgatgcgccc
    18901 ttcatcgtcc aggtctggga aaaccgcctg ttcgacctcg tcttgcagat ggtggccctg
    18961 attgccggcg tcatgggcgt gctcagcctg ctgggcgaga cgcgcgccac cggcacgacc
    19021 gcgaatgagc cgacgccgcg catggaggga gagcaatgac cccaatccaa atcaccatcg
    19081 ctggcgcgat cctgctggtg gccgtgggtc tttatggcct gttggtcagc cgccatctca
    19141 tcaaggtcgt cattgccctg caaatcatgg tcaagggagc catgctggcc atcatcgttg
    19201 ccggcgcggc tgttggccag atgaacctgg cccagagcat ggccctgacc gtcatcgtcg
    19261 tcgataccat tgccgccgtc ctggccctgg cgctcatcgt ccagatcaag cgccgcacgg
    19321 gtacgctgga cgcggccgag ttgacccaac tgcgccatta ggttgagcca acgctcgccg
    19381 tcaagaggac gatatggttc caacgctcat tctgatcgcc atcggcgcgc cctggctcgg
    19441 cgcgctggtc gtgtggctcc ttggcgatga tcggccgcgc tggcaacacg ggctggcgtc
    19501 ggccgcggcg ctggcggcgg ccggcgcggc gctactgctg ctgccggccg gccgctgggc
    19561 ggccacggac gaggtcgccc tgagcttgtc cttcggaccg ttcattggcg acatgacttt
    19621 cgtcgccgac agcctggccg ttttcctggc ggtcatcgcc acgaccatcg gcgcgctgac
    19681 ggtcattttt tcggtgggct atatggccag cgaagcccaa ctcggccgct actacgccct
    19741 cgtgctggcc ttcatcggcg cgatgtgtgg cctggtgctc agtggtaatc tgctcttcct
    19801 gttcctgttc tgggaagtga ccgctttctg ctcctacgcg ctcatctcgt tctacaacga
    19861 cgacccgcgg gcggtggccg ccggcctcaa ggcgctgatc atcacccagt tgggcggcgt
    19921 cgggttgctg gtgggcgtgc tgatggcttc agcttacctg cccgatttgc aggtcagtac
    19981 gctgctggcg tcgttcggcg atctgcccgc cgggacgctg gcggtggtgg ccttcggctt
    20041 cctgctggcg gccatggcca agtcggcgca agcgccgctg cacatctggc tgcccgacgc
    20101 gatggaagca ccgacgccgg tcagcgccct gatccacgcg gcgacgatgg tcaacgccgg
    20161 cgtctacctg ttggcccgct tctacccggc attcgccggc gtggagggct ggacgacggc
    20221 cgtggtcatc gtcggcctgg cgtcggcgct gctgggcggg gtgctggcca cgacgtgcaa
    20281 tgacctgaag cgcacgctgg cctattcgac cgtcagccaa ctcggcttca tggtcaccgg
    20341 cgtcggcctg ggggccatct tcgagagcca gttctacctg ctcagccacg ccatcttcaa
    20401 ggcgctgctg ttcctggggg cgggggcgat catccacgcc gtgggcacgc gcgacatgcg
    20461 ccaaatgggc ggtctgtggg ggcggatgcg tgtcgtgtcc gtctgcttcc tcatcggcgc
    20521 ggcggcactc atggggctgc cattcttcaa cggcttctgg agcaaggaga ttctgctgga
    20581 gacggcgctg gagcagaacc cggccgtctt cctggcgttg ctgctgggcg cggcgctgac
    20641 ggccttctac gccgtgcgca tgtgctggct ggtgttcttc cgccagccgg aaaccgccgc
    20701 ggccacgtct catgcccatg cggatggcca tgcccaggta tcgccgttca t
//
LOCUS       DAOSMQ010000004        20447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_2612_length_20447_cov_4.563505, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000004 DAOSMQ010000000
VERSION     DAOSMQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 20447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20447
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2612_length_20447_cov_4.563505"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PK829_00280"
     CDS             complement(<1..597)
                     /locus_tag="PK829_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39683.1"
                     /translation="MLGVGVGLAVLALMQVGQAQAAGNVPALSFLGEGGTYSVGEGYS
                     FLVKAKHPFHFMSISGPEYTARADERVWAVLGDFDTPVAEYQTTTIVGPVEKGCVIDF
                     ATVDDDEDDRINYFLLDGGVIYTMGQGMSTRGRFFVPRDGVLSYEANDSIGMFLSVCD
                     KKVTLTPTMTLTPTATLEPTLTPTPTLTLETGSSDAVVN"
     gene            937..2313
                     /locus_tag="PK829_00285"
     CDS             937..2313
                     /locus_tag="PK829_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU39684.1"
                     /translation="MISDRTPTYNLKAVVQETGLKPDTLRAWERRYGIPDPDRTDGGH
                     RLYSQHDINTLKWLLARQEEGLSISRAVALWHSLRAEGRDPLVVYTLSSSREADSYTT
                     VAPGSAMIDLRRAWIDACLAYDERRAESTLNQAFAYYAPEDVCFELLQKALAEIGDRW
                     YEGNVSIQQEHFMSALTMRRLDALLAATPAPTRAGRLLIGCPPGENHTFSPLLLTFLL
                     RRRGWDVLYLGADVPTDRLAMTIESTRPDLVIFSAQMLDTAASLYDVAVYLRAAGIAL
                     AYSGYIFGLVPELTHRIPGHFLGYDLRRTPAIIENLLADRPPAPPTEPVPESYSHAWA
                     YFRERSGLIETHIWQPLNGNAALNGTAEAIRRLTNNILSALKLGDLDYIAPNLQWLVG
                     MMSSLNLPADTLCHFLTAYHAATAQHLDATLGQPVIAWLAKVVAECRAQSSQEAKGQA
                     SVPSEEAR"
     gene            2310..3245
                     /locus_tag="PK829_00290"
     CDS             2310..3245
                     /locus_tag="PK829_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOU39685.1"
                     /translation="MTKRTIITGGSGVIGRRLSTELMAAGHEVIVLSRNPSPQKTVAG
                     VQAVTWDARTAAGWGHLADGAFAIINLAGESISGNNTLPLPGTWSVERKKRIKESRRH
                     AGEAVVAAVAAATVKPQVVVQMSGIDYYPSGDQVMTEESPRGRQFLADVVADYWEPAT
                     APVEELGVRRVVTRMAPLLHRETGPLPASLLQFKLFAGGRLGSGRQWLSWIHLEDTVR
                     ALRFLAECETAAGVYNLAAPNPVTNAEFTRVLGRIMHRPALLPVPKFALKLALGELSA
                     LVLDGRPVSVAKLQALGFTFRFPTLEAALRDILHK"
     gene            3254..4657
                     /locus_tag="PK829_00295"
     CDS             3254..4657
                     /locus_tag="PK829_00295"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOU39686.1"
                     /translation="MSDTVLWWLRRDLRLDDNDALRAALAGGRRVVPVFVLDPVLLDG
                     PTSSPTRTAFLLGGLRALDASLRARGSRLVARRGRPVEVLARLLAETGATAIYAEDDH
                     SPYAVRRDSEVGRQLPVHFFGSSAIRPPGTVLKADGSPYTVFTPFSRAWLAQPLPGRG
                     DLWAAPSALPDPGDAAGEPLPATPALPAAVPFPPGEAEAQRRLRAFAAESIAAYDLLR
                     DRVDMEGTSRLSPYLRFGMVAPRAAAVAALAAQAEQTGGGGAGRWLNELIWRDFYIHI
                     LAHFPAVAHESFRPAARAIAWRNDPREFIAWCAGETGYPIVDAAMRQLNTSGWMHNRA
                     RMIVASFLVKDLLIDWRWGERWFMRRLVDGDPAANNGGWQWTAGTGTDAAPYFRVFNP
                     VTQGRKFDPAGHYVRHWLPALAGVPDAFVHEPWRLDAAGQRAAGCVIGRDYPLPIVDH
                     ALARERVLAAYAAKATV"
     gene            4678..5364
                     /locus_tag="PK829_00300"
     CDS             4678..5364
                     /locus_tag="PK829_00300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOU39687.1"
                     /translation="MTRQPDIELFVDGRATRGYLARPAGDGPWPGVVVVQEWWGLNDH
                     IRDTADRLAGEGFVALAPDLYYGQTAREPDEARKLAMALEYPDALRVLQTAIDHLIGL
                     DDVAPKRVGIVGFCMGGGLAWHGAAQLSGLGAAVPCYGGGPEMTPAEAANISVPVLAI
                     YGELDRGVSPEVAWQRAALMDAAGVTNEMVIYPGAQHAFMNDTRPVYYPEAAEDAWRR
                     IVVLFHETLR"
     gene            complement(5473..5730)
                     /locus_tag="PK829_00305"
     CDS             complement(5473..5730)
                     /locus_tag="PK829_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOU39688.1"
                     /translation="MGLLGWLIGGAIVGWVASIITGRNAQQGLLGNIVAGIVGAFVGG
                     AIYGFLTGNGFNFEWSIGSFLVALVGAVIVLFLWNMISRRA"
     gene            complement(5960..6148)
                     /locus_tag="PK829_00310"
     CDS             complement(5960..6148)
                     /locus_tag="PK829_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39689.1"
                     /translation="MSKKKVVLTEEQRKRNAEILARLEEERVRLIEETRALQQQERRR
                     PGRKRKKKSLDELALATD"
     gene            6757..7476
                     /locus_tag="PK829_00315"
     CDS             6757..7476
                     /locus_tag="PK829_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39690.1"
                     /translation="MSTPMFIWLGSGRARRRHVGAPALWLDRAANAGLPVPPGAVVLD
                     EFFRSALDLGLATESDGRVAIPDAELWHNTLLDSAHLPRFRRPVNLYSVPATGCTTEA
                     VPIAHEVDFGDAHAAARAFAAIWSRRQERRDTLIVENIVAAAAGTATTTAGVDAPSDV
                     VTRDNSTFLLPQLRGWRGPDKAQPLYARRLQQLLRGVRRTLGPGVWRVHWADDDHICW
                     LVLIAAEVDERPRQRTPDPAV"
     gene            7528..8511
                     /locus_tag="PK829_00320"
     CDS             7528..8511
                     /locus_tag="PK829_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOU39691.1"
                     /translation="MNHSPLRLLVLIVLCFGALTACNRSNPPVATLAPTAVVPEQSTG
                     DTTDTGAAAAPTTAVTAVAEAARPSATPAAPAATATPAPTPTPEPSPTPAPPPVYTFE
                     IINTYPHDPTAFTEGLVFFDGTLYEGTGRWGESALREVALETGAVLRNLPLDAQYFGE
                     GIAVLNDRVYQLTWQEGTGFVYDRATFAPLQTFSYTHEGWGITHDGARLIMSDGTPVI
                     RFWDPETLQETGRITVRDNNGPVNRLNELEYVDGEIWANIWLTDLIARISPKTGDVLG
                     YIDLTGLLDTSGLTQPVDVLNGIAYDAAGGRLFVTGKLWPSLFEIRVVPQS"
     gene            8677..10770
                     /gene="uvrB"
                     /locus_tag="PK829_00325"
     CDS             8677..10770
                     /gene="uvrB"
                     /locus_tag="PK829_00325"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOU39692.1"
                     /translation="MSQFQLVSDFAATGDQPSAIADLVAGMNRGDKFQTLLGATGTGK
                     TFTIANIIQQTQRPTLILAHNKTLAAQLYSEFREFFPSNAVSYFVSYYDYYQPEAYVP
                     RHDLYIEKETQINEEINRLRLQAMATLKSRKDVIIVASVSCIYGIGNPEAWGNVIIDL
                     ERGSQYRRNTLLRHLVDIHYDRNDMELRRGTFRVRGDTLQVYPAYAETAYLVEFWGDT
                     IERIAEFDPLTGEVLLEHTRVSIYPAREFITDEEKLSHAITDIETELAERIAYFKNEK
                     RFLEAQRIEQRTMYDLEMLREVGFTPGVENYSRHLDQRPPGSRPWTLLDYFPADYLMV
                     IDESHMTIPQVRGMWGGDQSRKSILVDYGFRLPSAMDNRPLNFEEFESLLNQVIFTSA
                     TPSDYEFNHSAQVVQQIIRPTGIIDPEVEVRPVRGQVDDLIGEINRRIARGQRALVTT
                     LTKRMAEDLSDYLLELGIKVHYLHSEVATLERTEILRDLRLGVYDVVVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSETALTQTIGRAARHVDGKVIMYADVMTDSMKGAINTTN
                     ARRAIQHAYNVEHGIEPRSIVKAVHVLTDELTAQREMARGEMTPAIAESKGGYVTAAD
                     LPKAELAKIIAELEKQMKQAAQQLEFERAAILRDQVMEMRQIMILKEAGNKGSLPEWE
                     RMRLLDEAGVEYEVK"
     gene            complement(10811..12082)
                     /locus_tag="PK829_00330"
     CDS             complement(10811..12082)
                     /locus_tag="PK829_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOU39693.1"
                     /translation="MDEALLSRLRRLGVVKTARQIEPPAPASTRPRHRPLSLEQLLPG
                     GRVVDSDDGGCFVIDRVFVTDHRHGGDALADLLRSRPAAAVPYVPDARLEALDFRDFL
                     FLDTETTGLAGAGTLAFMVGVAYFEARPDGGDVLVVRQYFLRDHGDERAMLRQLDGLL
                     AERAGLITFNGRSFDVPLLDNRYLMNRLPSRLAQLPHIDLLLPARRLFRARLGSCALG
                     ALERNLLGLQRTQEDVPGALIPALYYQYLRTGEMGALASVFYHNQMDVLSMVTLAARI
                     FRQLDTSCCDDPLDQFSLARWQADLGLRERAERTLRASLAAEMPFETYHEGLQRLAAL
                     LKASGQRDEAVVVWQQIAHTSTSDVTAHVELAKHYEWVERDAAAALAWTERALALVAR
                     WTPTPNARLTRAELAHRRERLARKLGGQDSL"
     gene            12261..13592
                     /locus_tag="PK829_00335"
     CDS             12261..13592
                     /locus_tag="PK829_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOU39694.1"
                     /translation="MGADNPFFHRGAIRRVEDFVGRAAEINQTLGLLRNGQSVSLIGP
                     RRIGKSSLLLHLTHPTVRARYQLDPPHSLFVMLDCQELAGSPPEEVYELILDGLLEAA
                     TAAGLTVETATQPGTYRALDRALQQIYQHGVSVVVLLDEFELLAANLHLTPYFFARLR
                     GLTTKYGLAYLTASQRPLFAITAEEAVLSSPFFNIFVTVPLGLFNEEEARALFDKRLS
                     RTSVSFSEELTRYLLWLVGPHPFFLHIAGYHTVQVLAARDVRTAAQPVDFPLSNLDGP
                     IELESESHLGYLWQNLSAEEQHVLAIADGPVESLRQLEQQCLLERADGQYRYSSEILR
                     RFVRRQNVPGLVQAGPFVIDEQRHQVWGDNLELNLTASQFAILVRLCRNAGQVVRGED
                     LELAVWGDVLVDDPDRLKTLIKRLRRAVDPLGNWIVSERGVGYALRPPGED"
     gene            13690..18567
                     /locus_tag="PK829_00340"
     CDS             13690..18567
                     /locus_tag="PK829_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU39695.1"
                     /translation="MALLAALSAAVVLVPRAAAQSTGRAPGYWQFAASNRLDSVMLTD
                     IDADGVDDILVLDENGRLTLLSADGTPLWSFLSPDPVAAIGAAAVDSGLPQRSVAAAA
                     GQNLILLDGDGEERWRVPIDAPIAPAGIFAYDFEGDGNEEILVMLASGQLLTYDADGA
                     LLWQFSGQEDPTAAVNPQLIVSDLDGDGTQEIVLGLFTPRRFSQLIYLKGGVVQWRQS
                     VSRRITALAEAPFDNHASAIAVGTNFGQLNLYSLNGQLIWYRTLNRAITALAFANLSS
                     QPALAVGTDAGSIIAFSQEGRRLWANNLDRDAARGILALLPANDRILTDQPALAAILE
                     PASESSELADVFLLAGDGQTLGNPADTDLAGLTRLTDVNDDGYYELLLARFATLQLVG
                     LGIGDSEYVQEWNYALDAAPTAVLVLDLDGDGEDEVIVGTRDGRLHSLSIDRTIRWLN
                     APGEEIAFLAGAHSTPSQPPRVAVVRRKRALNDGVATGAGPSWLELREATGERLWAAA
                     IDAQITALTVDSQGDSDTSSIVVGTSNGEVFAYGFGGNLLWSHRFSDLAGGVRHLVYH
                     DQVGTEPGGLLVAGQRTIESLTPSAAGPISTVFVAFDQPIVALYSVPASPNRELAVSL
                     VVFLEDGSVHGLNGRGIEMGQWSWPFQLDARPTTTVTFGGSADEAFQENSNAFLVATD
                     DDRLQQLAIIDNLPVVSWQLGDLAAVQALAWSDLDKDGRPDTALVGTRDGGVWLYEQL
                     YTHTPQRILELPLASGIFQLALLPRTSSQSPDLLAITQNGLVRLFREEENRPPLLTEP
                     RVETEAEQYTIGVQVNDVENDAVNVRLELLEPVSGGWLPQTEQQLPTGNGRLVWPAVT
                     FPANTARIVYRFVYNDGFYTGTLTPPVGPEYVERGGRNVGLLLGSVVGVLLVAGLFLA
                     VRGAQTPAAQADRFYRQLGQNPTETLMRLEARYAVVGGSPDFLLQLANRARQANDVDL
                     ANMADGLFLLPNRPQAGLSIITRTLDDIHLVGRQWINLARRRLIYKTCQALLEAPSIT
                     ELGLLRPQFIHLLTILEDKREWSPVFEGLLPIITNMRDSERVDLVEDRLVYLNQAVAG
                     LRGVQEQLDGLGASVERTLLRAIVRRWSGLLTAEIEEQRGRAELEVALMTKRLAPNGR
                     THVALEIRNTGRAAAENLVATLDENPAYRVYGEPQIIPFLPSGRSRQVRFLIEPQAEA
                     RFRVGLSLTYDDHNRRDKSIAFGDMVYLLPPVREFTPIPNPYLPGTALRKNSPLFFGR
                     EELFDFIAENAGAQRQRNVLMLVGQRRTGKTSVLLRLEDYLPSSIVPVYIDCQSLGVS
                     AGMPALLQEFAWHIADALAARGLDCPVADRQRWQEDPARLFHREFLPTARRLLPEDTT
                     LLLVFDEFEAFESMIADGILPRTFFPYMRHLMQHSTGLGFVFVGTRRLEEMSADYWSV
                     LFNVALYRKIDFLSEPAALRLICEPVAPTLVYDDLALDKILRVTAGHPYFLQLVCYTL
                     VKQANQQKTGYVTISDVNTALDEMLRLGEVHFAYLWQRSSHAERALLAAAAHLMDRNE
                     PLHPEVFIEHLQPYNIELDPMQVTEALVSLVARDIMREVTEEGKALYELRIGLVGLWV
                     ARNKSLSRLHAHLES"
     gene            complement(18564..19052)
                     /locus_tag="PK829_00345"
     CDS             complement(18564..19052)
                     /locus_tag="PK829_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU39696.1"
                     /translation="MKTLLLMRHAKSSWDNNELSDFDRPLNSRGRRDAPRMGQLLAQE
                     DLTPDLIVTSAARRAATTAELVALAAGYGGDIHYTRELYLADTDTYLELAREIASDVN
                     RLLLVGHNPDMEALVSLLAGHEERMPTAALAHFRLAIDHWSALTEETGGELVALWRPK
                     EL"
     gene            complement(19121..19786)
                     /locus_tag="PK829_00350"
     CDS             complement(19121..19786)
                     /locus_tag="PK829_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOU39697.1"
                     /translation="MTHPWELAMLDDDDDDDFPPDIDPDEEPEEDDDDKPDDKRDGFG
                     SMEEKLLKNRTILIYGEINMELAMDITKRLLILDAESQDEIRIFINSPGGHVESGDTI
                     FDMIRFVKSPVKVIGTGWVASAGALIFAAAERENRYSLPNTRFLLHQPAGGASGQAAD
                     IAIEAQEIIKMRRRLNETFARQTGQPIEKVEEDTDRNFWMSAEQARDYGLVGKIIGTI
                     DEL"
     gene            complement(19880..>20447)
                     /gene="eno"
                     /locus_tag="PK829_00355"
     CDS             complement(19880..>20447)
                     /gene="eno"
                     /locus_tag="PK829_00355"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372163.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU39698.1"
                     /translation="MDPAASEFYEDGKYHLKVEGKALTGREMVDFWEWWLDKYPIISI
                     EDGLAEDDWDNWALMVERLGDRLQIVGDDLLVTNVKRIEQALERKAANSLLCKVNQIG
                     TLTEAIESVNLVQRNGWTAVTSHRSGETEDATIADLAVALNAGQIKTGAPARSDRIAK
                     YNQLLRIEEDLGDSAIYPGMKAFSNLRR"
BASE COUNT         3596 a         6872 c         6357 g         3622 t
ORIGIN      
        1 gttgaccacc gcatcgctgc tgccggtctc cagcgtcagg gtcggcgtcg gcgtcagagt
       61 cggctccagc gtcgccgtcg gcgtcagggt catcgtcggc gtgagcgtca ccttcttgtc
      121 gcagacgctc agaaacatac cgatgctgtc gttagcctca tagctgagga cgccgtcgcg
      181 cgggacgaaa aaccggccgc gggtgctcat cccctggccc atggtgtaaa tcacaccgcc
      241 atcaagcagg aagtaattga tgcggtcgtc ttcgtcgtcg tcaaccgtgg cgaagtcgat
      301 gacacagccc ttctccactg gtccgacgat cgttgtggtc tggtactcgg ctaccggtgt
      361 gtcgaaatcg cctagcaccg cccagactcg ctcgtcggcg cgagcggtgt attccgggcc
      421 agatatggac ataaagtgaa atgggtgctt ggctttcacc aggaagctgt aaccctcgcc
      481 gacactgtag gtcccgccct cgcccaggaa cgacagcgcg ggcacattgc cagccgcctg
      541 cgcctgcccc acttgcatca gcgccagaac agctaacccc actcccactc ccaacatcgc
      601 gatcgtaact acccttactg cgtacttctt catgacacta cccttaaact tcatgacgga
      661 tccgctacac acttttaagt ctaagaggta agaggaagca ctgtggacgg ttagcaacag
      721 aaaaagtgta aactggttta cacaagcgtg aacaactggc aaaatcgact cgcgtttgta
      781 atgcaatatg taatgggtag ctaggtcgct tggctcaggg gcgcaccctt aaggccagtc
      841 gcgccggtct atcgaacgaa atatggacaa atgcttgata tgctgaccgg ggtgtggtat
      901 gcttggccat aacaacacgg ctacagggca aacgcaatga tcagtgatcg gactccaacc
      961 tataacttaa aggccgttgt gcaggaaacc ggactaaagc cggacacgct gcgcgcctgg
     1021 gagcgtcgct atggcatccc cgacccagac cgcaccgacg gcggccatcg gctctattcg
     1081 caacatgaca ttaatacgct gaaatggctg ctggcgcgtc aggaggaggg gctgagcatt
     1141 agtcgcgccg tggccctatg gcactccctg cgagccgaag ggcgcgatcc gttggtcgtc
     1201 tacacgctgt cgtccagccg cgaagcggac agctacacga cggtcgcccc cggcagcgcc
     1261 atgatcgact tacgccgcgc ctggatcgac gcctgtctgg cctacgacga gcgccgggct
     1321 gaatcaacgc tcaatcaagc cttcgcctac tacgcgcccg aagatgtttg cttcgagttg
     1381 ctgcagaaag ctctggcgga gattggcgac cgctggtatg agggcaatgt ctccatccag
     1441 caagagcact tcatgtccgc gctgaccatg cgccggctgg acgcgctgct ggcggcgacg
     1501 cccgcgccca cacgcgccgg ccgcctcctc atcggctgcc ctcccggcga gaaccacacc
     1561 ttcagcccgc tgctgctcac cttcctgttg cggcggcgcg gctgggatgt gctctacctc
     1621 ggcgccgacg tacccaccga ccgcctggcg atgaccatcg aaagtacccg cccggacctg
     1681 gtcatattct cagcccagat gctcgacacg gccgcctccc tgtacgatgt ggccgtctac
     1741 ttgcgcgcgg ccggcatcgc cctggcgtat agcggttaca tcttcggcct tgtgcccgaa
     1801 ttgacccatc gcatccccgg ccacttcctg ggctacgacc tgcgcagaac gccggccatc
     1861 atcgaaaacc tgctggccga ccgcccgccc gcgccgccga cggagccggt gccagaaagc
     1921 tacagccacg cctgggccta ctttcgcgaa cggtcgggcc tgatcgagac gcacatctgg
     1981 cagcccctca acggcaatgc ggcgctcaac ggcacggccg aggccattcg ccgcctgacc
     2041 aacaacatcc tgtcggcgct caagttgggc gatctcgatt acatcgctcc caatctccaa
     2101 tggttggtgg gcatgatgag tagcctgaac ctgccggccg acacgttgtg tcactttctg
     2161 acggcctacc atgccgccac cgcgcagcac ctggatgcca cgctggggca gccggtgatc
     2221 gcctggctgg caaaggttgt cgctgagtgc cgcgcccaat caagccagga agctaagggc
     2281 caggcaagtg tcccttccga ggaagcaaga tgaccaaacg cactatcatt accggcggct
     2341 ccggcgtcat cggccgccgt ctgtccaccg aactcatggc cgccgggcat gaagtgatcg
     2401 tcctgagccg caacccatcg ccgcagaaga cggtggccgg ggtgcaggct gtcacttggg
     2461 acgcgcgcac ggcggcgggc tggggccatc tggccgacgg cgctttcgcc atcatcaacc
     2521 tggccggcga atcgatcagc ggcaacaata ctctgccact gccgggaacc tggtcggttg
     2581 agcgcaagaa gcgcattaag gagagccgtc gccacgccgg ggaggccgtc gtcgccgccg
     2641 tggcggcggc aacggtcaag ccacaggtcg tcgtgcagat gtcaggcatc gattactacc
     2701 ccagcggcga ccaggtcatg accgaagaat cgccacgcgg gcggcaattc ctggccgacg
     2761 tggtggccga ctattgggag ccggcgaccg cgcccgtgga ggaactgggt gtgcgccgcg
     2821 tggtcacgcg catggccccg ctgttgcacc gcgagacagg cccgctgccg gcctcgcttt
     2881 tgcagttcaa gctcttcgcc ggcggccggc tgggcagcgg ccggcaatgg ctgtcgtgga
     2941 tccaccttga ggacactgtg cgcgccctgc gctttctggc cgagtgcgag acggccgctg
     3001 gcgtctacaa cctggccgcg cccaacccgg tcaccaatgc cgagttcacc cgcgtcctgg
     3061 gccggatcat gcaccggcca gcgctgctgc ccgtgcccaa attcgccctg aagctggccc
     3121 tgggcgagct gtccgcgtta gtgctggatg ggcggccagt cagcgttgcc aagcttcagg
     3181 cgctgggctt caccttccgc ttcccgacgt tggaagcggc gctgcgcgac atcctgcata
     3241 agtgaggcca gcggtgagcg acacagtcct gtggtggctg cggcgagacc tgcgactgga
     3301 cgataacgac gccctgaggg ccgcgctggc cggcggccgg cgcgtcgtgc ctgtcttcgt
     3361 cctcgatccg gtcttgctgg acgggccaac gagtagccca acgcgcacgg cctttctgct
     3421 cggtgggctg cgcgccctcg atgccagctt gcgcgcgcgc ggcagccggc tggtggcgcg
     3481 gcgcggccgg ccggtcgagg tgctggcccg cctgctggcc gagaccggcg cgacggccat
     3541 ctacgccgag gacgaccatt ccccctatgc cgttcgccgc gatagtgagg tgggtcgcca
     3601 actgcccgtg cacttcttcg gcagcagcgc catccgcccg ccggggacgg tgctgaaagc
     3661 cgacggctcg ccctacaccg tcttcacgcc tttcagccgc gcctggctgg cccagccctt
     3721 gcccggtcgc ggcgacctgt gggccgcgcc gtcggcgctg cccgaccccg gcgacgcggc
     3781 cggcgaaccg ctgcccgcca cgccggcgct gcccgcggcc gtgcccttcc cgccgggcga
     3841 ggccgaggcc cagcgccgcc tgcgcgcctt cgccgctgag tccatcgctg cctacgatct
     3901 cctgcgcgac cgggttgata tggagggcac gtcgcgcctg tcgccctatc tgcgcttcgg
     3961 catggtcgcg ccgcgggcgg cggcggtggc cgcgctggcg gcgcaagcag agcagaccgg
     4021 cggtggcggc gcaggccgct ggctcaacga actgatctgg cgcgatttct acatccacat
     4081 cctggcccac ttccctgccg tcgcccacga gagcttccga ccggcggcgc gcgccatcgc
     4141 ctggcgcaac gacccgcggg agttcatcgc ctggtgcgcc ggcgagacgg gctaccccat
     4201 cgtcgacgcg gccatgcgcc agctgaacac gagcggttgg atgcacaatc gcgcccgaat
     4261 gatcgtcgcc tccttcctgg tcaaggattt gctcatcgac tggcgctggg gcgagcgctg
     4321 gttcatgcgc cggctggtgg acggcgaccc ggcggccaac aacggcggct ggcagtggac
     4381 ggcgggcacc ggcaccgacg ccgcgcccta tttccgcgtc ttcaacccgg tcacccaggg
     4441 gcgcaagttc gaccccgccg gccactacgt gcgccactgg ctgccggcgc tggccggcgt
     4501 gcccgacgcc ttcgtccacg agccgtggcg gctggacgcg gccgggcagc gcgccgccgg
     4561 ctgcgtcatc ggccgcgact acccgctgcc catcgttgac cacgccctgg cacgcgagcg
     4621 cgtcctggct gcctacgcgg ccaaagcaac tgtttgatcg ctcaggaggt ttcatccatg
     4681 acacgccaac ccgatattga gctttttgtt gatggccgcg ccactcgcgg ctacctggct
     4741 cgtcccgccg gcgatggccc ctggcccggc gtcgttgtcg ttcaggagtg gtgggggctg
     4801 aacgaccata tccgcgacac ggctgaccgg ctggccggcg agggcttcgt tgccctggcc
     4861 cccgatctct actatggcca gaccgcgcgc gaacctgacg aggcccgcaa gctggccatg
     4921 gccctggagt accccgacgc cctgcgcgtc ctccagacag ccatcgacca tcttatcggc
     4981 ctggacgacg ttgcgccgaa gcgcgtcggc atcgttggct tttgcatggg cggcgggctg
     5041 gcgtggcatg gcgcggcgca gctaagtggc ctgggcgcgg ctgtgccctg ctatggcggc
     5101 ggcccagaga tgacgccagc agaggcagcc aacatctccg tgcccgtgct ggccatctac
     5161 ggcgaactgg atcgcggcgt ctcgcccgag gtggcctggc aacgggcggc gctgatggac
     5221 gcggccggcg tgacgaacga gatggtcatc taccccggcg cgcagcacgc cttcatgaac
     5281 gatacccggc cggtttatta tcctgaagcg gctgaagatg cctggcggcg tatcgtcgtc
     5341 ctcttccacg aaaccttgcg ctagagcgga cgcccagagc cagaaagcaa aacggccccg
     5401 gctggatgtc gccaaccggg gccaagagga ccgcgaacga atgaatgaag gcagtaagac
     5461 tgctaccggc acttaggcgc ggcggctaat catattccac aggaacagca caatgaccgc
     5521 gccgaccaga gcaaccagga agctgccgat actccactcg aagttaaagc cgttgccggt
     5581 caaaaagcca taaatagccc cgccgacgaa cgcgccaacg atgccggcga caatattgcc
     5641 caacaagccc tgctgcgcgt tgcggccggt gataatgctc gctacccaac caacgatcgc
     5701 gccgccaatg agccaaccta gcagaccaat cattttgcca ctctcctctg tttatgaaca
     5761 gttcgcgtca aatcacacta acgtgttcgc ggtgattaac actggcattg tagacgcagt
     5821 tgtcggcctg ataaatatgc cctggatata aatgcgccag agaaaaaccc gcgctctctc
     5881 tggggagagc gcgggttggt atggacaaag ggcacaaatg ggatgcgggg ccagccgccg
     5941 gccagtcgtt gcgaggggat cagtcagtgg ccagcgccag ctcgtcgagg gacttcttct
     6001 tgcgcttacg ccccgggcga cggcgctctt gttgctgcaa cgcgcgtgtc tcttcgatca
     6061 atcgcacccg ctcttcctca agtcgggcca gaatctctgc gttgcgcttt ctctgctcct
     6121 ccgtcaggac caccttcttt ttggacatcc tcagtcaact ccttttgtcg ggtatgaaaa
     6181 catcgacagg tcggtcgccg cggggcagac agcgacggac aaccaacccg ttgtgagggc
     6241 taggggtgat agggccagga tcgaggtcgc gggcgggcgc gtccgccaat ggatagccgg
     6301 tcatgccctg gcgaatgggg cgaaagaatg agaggggcgg accctgccgt gttgcgccgg
     6361 gcaacaccaa caacaccatc ggtggacaac gcttttgcgg gcgtatgcac aatgctaacc
     6421 acgaatcctt cgcaactcag cgtctgtttc atgccgggca agggcacggc agcagtgccc
     6481 ttgtccaaac gcctgacatt cccggttagt agacagacgc atttattctt acgttattct
     6541 atcacaatct aatgttacat tccaatggga aattttacag acgagggttt ttcccaagtc
     6601 acccccggcg cagcgacctg acaggtaggc ggccacgaat gggactgcca ccggagaacg
     6661 actggccgcc caccccacct gtccggtcaa tggccgctct cgcttctcag atgattcacc
     6721 agcaacctat cccggactta tgatagaatc gggccaatgt caacgccgat gttcatttgg
     6781 ctcggttccg ggcgggcgcg acgccggcat gtcggcgcgc cagcgctctg gctcgaccgg
     6841 gcagcgaatg ccgggctgcc cgtgcccccg ggcgcggtcg tgctggacga gttcttccgc
     6901 agcgccctcg acctgggcct ggcgacggag agcgacggcc gggtagcgat tcccgacgcg
     6961 gagctgtggc ataacaccct gctcgacagc gcccacctgc cccgcttccg acggccggtc
     7021 aatctctact cggttccggc gaccggctgc acgactgagg ccgtccccat cgcccatgag
     7081 gtcgactttg gcgacgccca cgccgcggcg cgcgcctttg ccgccatctg gtcgcgcagg
     7141 caggagcggc gcgacacgct catcgtcgag aacatcgtcg ccgcggcagc tggcacggcc
     7201 acgaccacag ccggcgtcga tgcgcccagc gacgtcgtga cgcgcgacaa ttccaccttt
     7261 ctgctgcccc agttgcgtgg ctggcgcggc ccagacaagg cccagccgct ctacgcccgc
     7321 cgtttgcaac agctgctgcg cggcgtgcgc cgcacgctcg gcccgggcgt ctggcgggtc
     7381 cactgggccg acgacgacca tatctgctgg ctggtgctca tcgcggctga agtggacgaa
     7441 cgaccgcgac agcgaacccc ggacccggct gtctgaggca tggttgcgcc acacagccac
     7501 acccgcggcc ctcaaaggaa tcaatacatg aaccattctc ccttgcgctt gctcgttctg
     7561 atcgtcctat gctttggcgc attgaccgct tgtaaccgca gcaacccgcc ggtggccacg
     7621 ctcgccccca cggcggtcgt gcccgagcaa tcgaccggcg acacgactga tactggcgca
     7681 gcagcggctc cgacgacggc cgttaccgcc gtcgctgaag ccgcgcgccc ctcggccacg
     7741 cccgcggcac ccgccgccac ggccaccccc gcgccgacgc ccacgccgga gccatcgccg
     7801 acgcccgcgc cgccgccggt ctatacattc gagatcatca acacctaccc tcatgacccc
     7861 accgccttca ccgaaggtct cgtcttcttc gacgggacac tatatgaagg caccggccgc
     7921 tggggcgagt ccgccctgcg cgaggtcgct ctggaaacgg gcgcggtctt gcgcaatctg
     7981 ccgctcgacg cgcagtattt cggcgagggc atcgccgtcc tcaacgaccg cgtctaccag
     8041 cttacctggc aggaaggcac tggctttgtc tatgaccgag ccacgttcgc gccactgcaa
     8101 acctttagct acacccacga gggctggggc atcacccacg atggcgcgcg cctgatcatg
     8161 agcgacggca cgccggtgat tcgcttctgg gacccagaaa cgctccagga gacgggtcgt
     8221 atcaccgtgc gcgacaacaa cggccccgtc aaccggctga acgaactgga gtacgttgac
     8281 ggcgaaatct gggccaacat ctggctgacc gatctcatcg cccgcatttc gccgaagacg
     8341 ggcgacgtat tgggctatat cgacctgacc gggctactgg acacaagcgg gctgacccag
     8401 ccggtcgatg tcctcaacgg cattgcctat gacgcggccg gcggccggct gttcgtcact
     8461 ggcaagctat ggccatcgtt gttcgagatt cgggttgtgc cgcagtcgta gggcgatttg
     8521 gcaaatcgcc ccacaacctt accgtagggc gatttggcaa atcgccccca tctcaccgta
     8581 gggcgatttg gcaaatcgcc ccacaaagat gtagaatacg aactttcgtt ctacttagcg
     8641 cggcgcttat tggccagact tccgacgagg tgaagcatgt cccagtttca attggtttcc
     8701 gactttgccg ccaccggcga ccagccgtca gcaatcgccg acctggttgc cggcatgaac
     8761 cgcggcgata agttccagac gctgctcggc gcgacgggca cgggcaagac attcaccatc
     8821 gccaacatca tccagcagac acagcggccg acgctgatcc tggcccacaa caagaccctg
     8881 gccgcccaac tctactccga gttccgcgaa ttcttcccca gcaacgccgt ctcctatttc
     8941 gtcagctact acgactacta ccagccggag gcctacgttc cccgccacga cctctacatc
     9001 gaaaaggaaa cgcagatcaa cgaggagatc aatcgcctgc gtctgcaagc gatggctacg
     9061 ctcaagagcc gcaaggacgt catcatcgtc gcctcggtgt cgtgcatcta cggcatcggc
     9121 aatccggagg cctggggcaa cgtcatcatc gacctggaac gcggcagcca gtatcgccgc
     9181 aacacgctgc tgcgccatct ggtggacatc cactatgacc gcaacgacat ggaactgcgt
     9241 cgcggcacct tccgcgtgcg cggcgatacg ctgcaagtct accccgccta tgccgagacg
     9301 gcctacctgg tcgagttctg gggcgacacc atcgagcgca ttgccgagtt cgacccgctg
     9361 accggcgaag tattgctgga gcatacccgc gtgtccatct acccggcgcg cgagttcatc
     9421 accgacgagg agaagctgtc ccacgccatc accgatatcg agacagagtt ggccgagcgc
     9481 atcgcctact tcaagaatga gaagcgcttc ctggaggcgc agcgcatcga gcagcggacg
     9541 atgtatgacc tggagatgtt gcgcgaggtc ggtttcacgc ccggcgtaga aaactacagc
     9601 cgccaccttg accagcgtcc gcccggctcg cgtccctgga cgctgctaga ctacttcccg
     9661 gccgactatt tgatggtcat cgacgagagc cacatgacca ttccgcaggt acgcggcatg
     9721 tggggcggcg accagagccg caagagcatc ctggtcgatt atggcttccg cctgcccagc
     9781 gctatggaca accgcccgct caacttcgag gagttcgaga gcctgctcaa tcaggtcatc
     9841 ttcaccagcg ccacgccgag cgactacgag ttcaatcact cggcccaggt ggtgcaacag
     9901 atcatccggc cgacgggcat catcgacccg gaggtcgagg tgcggccggt gcgcggacag
     9961 gtagacgacc tcattggcga gatcaaccgg cgcattgcca gaggccagcg cgccctggtg
    10021 acgacgttga ccaagcgcat ggccgaagac ctgagcgact acctgctgga attgggcatt
    10081 aaagttcatt acctgcactc agaggtggcc acgctggagc gcacggagat tctgcgcgac
    10141 ctgcggctgg gcgtctacga tgtcgtcgtc ggcatcaacc tcctgcgcga ggggctggac
    10201 ttgcccgagg tgtcactggt ggccatcctc gacgccgaca aggagggctt cctacgctcg
    10261 gagacggccc tgacgcagac gatcggccgc gccgcccgcc acgtggacgg caaggtgatc
    10321 atgtacgccg acgtgatgac tgactccatg aaaggcgcga tcaacaccac caacgcccgc
    10381 cgcgccattc agcacgccta caacgtggag cacggcatcg agccgcgcag catcgttaag
    10441 gccgtgcacg tcttgacgga tgagttgacc gcccagcgcg agatggcccg cggcgagatg
    10501 accccggcga ttgccgagtc gaagggtggc tacgtaaccg ccgccgacct gcctaaagcc
    10561 gagctggcca agatcatcgc cgaattggag aagcagatga agcaggccgc ccaacaattg
    10621 gagttcgagc gcgccgccat cctgcgcgat caggtgatgg agatgcgtca gatcatgatc
    10681 ctgaaggaag ccggcaacaa gggcagcttg ccggagtggg agcgaatgag gctgctggat
    10741 gaggcgggcg tcgagtacga agtgaaatag cgctcagcgc gctcttgcat tcccacgagc
    10801 ggggggacgg ctagagcgaa tcctgtccac ccaactttcg cgccagccgc tcccgccggt
    10861 gggccaattc tgcccgcgtc aggcgggcgt tgggcgtcgg cgtccagcgg gcaacgagag
    10921 ccagcgcgcg ctccgtccag gccagcgccg cagccgcgtc acgttccacc cactcatagt
    10981 gcttggccaa ctccacgtgg gcggtcacgt cgctggtgct ggtgtgggcg atctgctgcc
    11041 agacgacaac ggcctcatcg cgctggccgc tggccttcag cagcgccgcc agccgctgta
    11101 gcccctcgtg gtaggtctcg aaaggcatct ccgcggcgag cgaggcgcgc agggtgcgct
    11161 ccgcccgctc gcgcagaccc aggtcggctt gccagcgcgc caggctgaac tggtcgaggg
    11221 gatcatcgca acacgacgta tctagctggc ggaagatgcg cgccgccagc gtcaccatcg
    11281 acagcacgtc catctggttg tgatagaaca cgctcgccaa tgcgcccatc tcgccggtgc
    11341 gcaggtactg gtagtaaagg gccgggatga gcgcgccggg cacatcttct tgcgtccgct
    11401 gcaagcccag caggttgcgc tcgagcgcgc ccagcgcaca ggagccgagc cgggcacgga
    11461 agaggcggcg cgccggcaac agcaggtcga tgtggggcag ttgggccagg cggctgggca
    11521 gccggttcat caggtagcgg ttatccagca ggggcacgtc gaaggagcgg ccgttgaagg
    11581 tgatcagccc ggcccgctca gccagcagcc catccagttg acgcagcatg gcccgctcgt
    11641 cgccgtggtc gcgcaggaag tattgccgca cgaccaggac atctcccccg tcgggccgcg
    11701 cctcgaagta agccacgccg accatgaagg ccagtgtgcc cgcgccggcc agcccggtcg
    11761 tctccgtgtc cagaaagagg aagtcgcgga aatcgagggc ctccagtcgg gcgtcgggca
    11821 cgtagggtac ggccgcggcc gggcggctgc gcagcaggtc ggccagggca tcaccgccgt
    11881 ggcgatggtc ggtgacaaag acgcggtcga tgacaaagca gccgccgtcg tcagaatcga
    11941 cgacgcgccc gccgggcaag agctgctcca gcgacagcgg ccggtggcga gggcgggtgc
    12001 tggccggcgc aggcggctca atttgccgcg ccgtcttgac cacaccaagt ctgcgcaatc
    12061 gcgacaggag cgcttcgtcc atatgttcgg attttacgcc gctcgcccta tggtgacaag
    12121 gggcgttgtg aggtcggtca agggctgacc caactggcca ggtgaccact cctcctctca
    12181 ggcgcgagat gggcgttgtc ggcgtatcat tgtgccgtca ttaccctttg gcggtcttcg
    12241 ggattacaat aggggtaact atgggtgcag acaatccctt tttccatcgt ggcgctattc
    12301 gccgggttga ggacttcgtt ggccgcgcgg cggagattaa ccagaccctg ggcctgttac
    12361 gcaacggtca aagcgtgtca ttgatcgggc cgcggcgcat cggcaaaagt tcgctgctgt
    12421 tgcacctgac ccaccccacc gtgcgcgccc gctaccaact tgacccaccc cactcgctat
    12481 tcgtcatgct tgactgccaa gagctggccg ggtcgccgcc ggaagaggtc tatgagctga
    12541 tcctcgacgg gctgctagag gcggccacgg ccgcggggct gactgtggag acggccacgc
    12601 agccgggaac ctaccgcgcc ctcgaccgcg ccttgcaaca gatctatcag cacggcgtca
    12661 gcgtcgtggt gctgctggac gaattcgagt tgctggccgc caatctgcac ctgacgccct
    12721 acttctttgc ccgcctgcgc ggcctgacga ccaagtacgg cctggcctat ctgaccgcca
    12781 gccagcgccc gctcttcgcc atcaccgccg aggaggccgt ccttagctca ccctttttca
    12841 atatcttcgt taccgtgccg ctaggcctgt tcaacgaaga ggaagcgcgc gccctattcg
    12901 acaagcggct gagccgcact tcggttagct tctccgagga gctgacccgc tatctgctct
    12961 ggctggttgg gccacatccc tttttcctcc acatcgctgg ctatcacacc gtgcaggtgc
    13021 tggccgcccg cgatgtccgg acggcggccc agccggtcga tttccctctg tcgaatctgg
    13081 acgggcccat cgaactggag tcggaatcgc acctcggcta cctgtggcag aacctttcgg
    13141 ccgaggaaca gcacgtcctg gccatcgccg acgggccggt ggaaagcctg cgccaactgg
    13201 agcagcaatg tctgttggag cgcgccgacg gccagtatcg ctatagcagc gaaatcctgc
    13261 gccgcttcgt gcggcggcaa aacgtgcctg ggctggtgca agccgggccg ttcgttatcg
    13321 acgagcagcg gcatcaggtc tggggcgaca atctggaact gaacctgacg gcgtcgcaat
    13381 tcgccatcct cgtccgtctg tgccgcaacg cgggccaggt cgtgcgcggc gaagacctgg
    13441 aactggccgt atggggcgat gtcctcgtgg atgaccccga ccgccttaag acactgatca
    13501 agcggctgcg tcgcgccgtc gatccgctgg gcaactggat cgtcagcgag cgcggcgtcg
    13561 gctatgccct gcgtccgccg ggcgaggact aggccccctc tcgcaagcgt actggtgaac
    13621 cgcatccgtt tcccacaagg aggccctggc gacagagcgc gccgcgctcg gttgtggctc
    13681 ctccacgggt tggcgctgct ggcagccctg tcggccgcgg tggttcttgt gccgcgcgcc
    13741 gctgcccagt ccaccggccg cgcgcccggc tactggcagt tcgccgcctc caaccggctc
    13801 gactcggtga tgctgaccga catcgacgct gacggcgtcg atgacattct tgtgcttgat
    13861 gagaacggcc gcctgaccct cctatcggcc gatggcacac cgctgtggtc attcctaagc
    13921 cccgaccccg tggccgcgat cggcgcggca gccgtcgata gcggtctgcc acagcgctcg
    13981 gtcgctgcgg ccgccgggca aaacctcatc ctgctggacg gtgacggcga agagcgttgg
    14041 cgtgttccca tcgacgcgcc catcgctcca gcgggcatct tcgcctacga ctttgagggt
    14101 gatggcaacg aagagattct ggtcatgctg gcatcgggac agcttctgac ctatgacgcc
    14161 gacggcgcgc tcctgtggca gttcagcgga caggaagacc cgacggcggc ggttaacccg
    14221 cagttgatcg tctctgatct ggacggtgac ggcacgcaag agattgtgct aggactattc
    14281 acgccgcgcc gcttcagcca gctcatctac ctgaagggcg gcgtcgtcca atggcgacag
    14341 tccgtctcgc ggcgcatcac cgcgctggcc gaagcgccgt tcgacaatca cgccagcgcc
    14401 atcgccgtag gcactaactt cggccaactc aacctctata gcctgaacgg ccagctgatc
    14461 tggtatcgca ctctcaaccg ggcaattact gccctggcgt ttgctaacct gagcagccag
    14521 ccggccctgg ccgtcggcac agacgccggc tccatcatcg ccttttcgca ggagggtcgg
    14581 cggctgtggg ccaataacct cgatcgcgac gccgcccgcg gcattctggc gctcttgccg
    14641 gccaacgacc gcattctgac cgaccagcct gcccttgcag ctatcctgga gcctgcctcg
    14701 gagtccagcg agctggccga tgtcttcctg ttggccggcg acgggcaaac cctgggcaac
    14761 cccgccgaca ccgacctggc cggcctgact cgcctgaccg acgtcaacga cgacggctac
    14821 tatgagctac tgctggcgcg cttcgctacg ctgcaactcg tcgggctggg catcggcgac
    14881 agcgagtatg tgcaggaatg gaactacgcc ctggacgcag cgccgacagc ggtcttggtg
    14941 cttgacctgg atggcgatgg cgaagacgag gtcatcgtgg gcacgcgcga cggccggctg
    15001 cacagcctaa gcatcgaccg caccatccgt tggcttaacg cgccgggaga ggagatcgcc
    15061 ttcctggccg gcgcgcattc cacgcccagc caaccaccgc gcgtggccgt tgtccgccgc
    15121 aaacgcgccc tgaacgatgg cgtggcgacg ggggccggcc ccagctggct cgaactgcgc
    15181 gaagcaacgg gcgagcgtct atgggcggcg gccatcgacg cgcagataac ggccctgacg
    15241 gtagactcgc aaggcgacag cgacacgtcg agcatcgtcg tgggcacaag caatggggaa
    15301 gtatttgcct atggctttgg cggcaacctg ctgtggagcc accggtttag cgacctggct
    15361 ggcggtgtgc gccatctggt ctaccacgat caagtcggca cggaacccgg cggcctgctc
    15421 gttgccggac agcgcaccat cgagagcctt acgcccagcg ctgccggccc gatcagcacg
    15481 gtgttcgtcg cctttgatca gcctatcgtc gcgctctaca gcgtgccggc ctcgcccaac
    15541 cgggaactgg cggtcagtct ggtcgtcttt cttgaagacg gctccgtgca tggccttaac
    15601 ggccgcggca tcgagatggg ccagtggtcg tggccgttcc agctagacgc ccggccgacg
    15661 acgaccgtca ccttcggcgg cagcgccgat gaggcctttc aggagaacag caacgccttc
    15721 ctcgtggcca ccgacgacga ccgcttgcag caactggcca tcatcgataa cctgcccgtc
    15781 gtctcctggc agctgggcga tctggccgcg gtgcaggccc tggcctggag cgacctggac
    15841 aaggacggcc gccccgatac cgctctggta ggcacgcgcg acggtggcgt ctggctctac
    15901 gagcaattgt acacccacac gccccagcgc atcctggaat tgccgctggc cagtggcatc
    15961 ttccagttgg cgctcttgcc gcgcacgtct agccagtcgc ccgacctgtt ggccattacc
    16021 caaaatggct tggtacgcct cttccgcgaa gaggagaacc ggccgccctt gttgacagag
    16081 ccgcgcgtcg agaccgaggc cgagcagtat accatcggcg tacaggtcaa cgatgtagag
    16141 aacgacgccg tcaatgtccg tttagagttg ctcgaacccg tcagcggcgg ctggttgcct
    16201 cagacagagc agcaactgcc cacgggcaac ggtcgccttg tctggccggc ggtcaccttc
    16261 ccggccaaca cggcgcggat tgtctatcgc ttcgtctaca acgatggctt ttacacgggc
    16321 accctcaccc cgccggtcgg gccagagtac gtcgagcggg gcggccgcaa tgtcggtctg
    16381 ctattgggca gtgtggtcgg cgtcttgttg gtggccgggc tgtttctggc cgtgcgcggg
    16441 gcgcagacgc cagccgcgca ggccgaccgc ttttaccggc aactggggca gaacccgacc
    16501 gagacgctca tgcgcctgga ggcccgctat gccgtcgtcg gcggctcgcc tgacttcctg
    16561 ctacagttgg ccaaccgcgc ccggcaggcc aacgacgtcg atctggccaa catggccgac
    16621 ggcctgttcc tgctgcccaa ccggccacaa gccggcctat cgatcatcac ccgcacgctg
    16681 gacgacatcc acctggtcgg ccggcagtgg atcaacctgg cccgccggcg gctcatctat
    16741 aagacctgcc aggcgctgct ggaagctccc tctatcaccg agctgggctt gctgcggccg
    16801 caattcatcc atctgctgac catcctggag gacaagcgcg agtggtcgcc tgtcttcgag
    16861 ggccttctac caatcatcac caatatgcgc gacagcgagc gggtcgattt ggtcgaagac
    16921 cggctggtct atctgaatca ggcggtagcc gggctgcggg gggttcagga gcagttagac
    16981 gggctgggag cgagcgtgga gcggacgctg ctgcgagcca tcgtccgccg ctggtcgggc
    17041 ctgctaacgg ctgagatcga ggagcagcgc ggccgggccg agctggaagt cgccctaatg
    17101 accaagcggc tggcccccaa cggccgcacc cacgtcgccc tggagattcg caacacgggc
    17161 cgggccgcgg ccgagaacct cgtggccacc ctggatgaga atccggccta tcgcgtctat
    17221 ggcgagccac agatcattcc tttcctgccg tcgggtcggt cgcggcaagt gcgcttcctc
    17281 atcgaaccgc aggcagaggc gcgtttccgt gttggcctgt cgctgaccta cgacgaccac
    17341 aaccggcgcg acaagagcat tgccttcggc gacatggtct acctgctgcc gcccgtgcgc
    17401 gaattcacgc ccatccccaa cccctatctg ccgggcacgg cgctgcgcaa gaacagccct
    17461 ctattcttcg gccgcgaaga gttgttcgac tttatcgccg agaacgccgg cgcccagcgc
    17521 caacgcaacg ttctcatgct cgtcggccag cggcgcacgg gcaagacctc ggtgctgctg
    17581 cgcctggagg actacctacc ctcgtccatc gtgccggttt acatcgactg ccaatccctg
    17641 ggcgtctcgg ccggcatgcc cgcgctcttg caggagttcg cctggcacat cgccgatgca
    17701 ttggccgcgc gcgggctaga ttgtcctgtg gccgaccgcc agcgctggca agaggacccg
    17761 gcccggctct tccaccgcga gttcttgccc acggcgcgtc gtctcttgcc ggaggacaca
    17821 acgctgctgc tggtcttcga cgagttcgag gccttcgagt cgatgatcgc cgatggcatt
    17881 ctgccgcgga cgttcttccc ctacatgcgg catctgatgc agcacagcac cggtctgggc
    17941 ttcgtcttcg tgggcacgcg ccgcctggag gagatgagcg ccgactactg gtcagtgttg
    18001 ttcaacgtcg ccctctaccg caaaatcgac ttcttatcgg agccggcggc gctgcgcctc
    18061 atctgcgagc cggtcgcgcc caccctggtc tacgacgatc tggcgctaga caaaatcctg
    18121 cgtgtcaccg ccggccaccc ctacttcctg caactggtct gctatacact ggtgaagcag
    18181 gccaaccagc aaaagactgg ctacgtgacc atctccgacg tcaacacggc gctggacgag
    18241 atgctgcgtc tgggcgaggt gcactttgcc tacctctggc agcgctccag ccacgctgag
    18301 cgggccttgc tggctgccgc cgcccacctg atggaccgca acgagccgct gcacccggag
    18361 gtgttcatcg agcacttgca gccctacaac atcgagttgg acccgatgca ggtaactgag
    18421 gcgttggtct ccctggtggc gcgcgacatc atgcgcgagg tgaccgaaga gggcaaggcg
    18481 ctgtatgagc tgcgaatcgg tttggttgga ttgtgggtgg cgcgcaacaa aagtctgagc
    18541 cggctgcacg cccatttaga gagctagagt tccttgggcc gccagagggc gaccagttcg
    18601 ccgcctgtct cctcggtcag cgcgctccaa tggtcgatgg ccagccggaa gtgggccagc
    18661 gcggccgtgg gcatgcgctc ctcgtggccg gccagcagcg acaccaacgc ctccatgtcc
    18721 gggttgtggc ccaccagcag cagccggttg acgtcactgg ctatctcacg tgccagttcc
    18781 agataggtat ccgtatcggc caggtagagt tcgcgggtgt aatgaatgtc gccgccatag
    18841 cccgcggcca gggcgaccag ttcagctgtg gtggccgcgc ggcgggcggc tgaggtgacg
    18901 atcagatcgg gcgtcaggtc ttcctgggcc agcaattggc ccatacgcgg cgcgtctcgc
    18961 cggccacgac tgttcaacgg ccggtcgaag tcggacaact cgttgttgtc ccaactcgac
    19021 ttggcatggc gcatcagcag gagtgtcttc ataagcaatg gccgccagac gggcggcgcg
    19081 acgcgccgtc cccgtctggc ggggccgata ggggctaggc ctagagttcg tcgatggtgc
    19141 cgatgatctt gccgacgaga ccgtagtcgc gcgcctgctc ggccgacatc cagaagttgc
    19201 ggtcggtgtc ttcctcgacc ttctcaatcg gctggccggt ctgccgggcg aaggtctcgt
    19261 tgaggcggcg gcgcatcttg atgatctcct gcgcctcgat ggcgatgtcg gccgcctgac
    19321 cgctggcgcc gccggctggc tggtgcaaga ggaagcgcgt gttgggcaag ctatagcgat
    19381 tctcgcgctc ggccgcggcg aagatcaatg ccccggcgct ggccacccaa cccgtgccga
    19441 ttaccttgac cggcgacttc acaaagcgaa tcatgtcgaa gatagtgtcg cccgattcta
    19501 cgtgcccgcc gggcgaattg atgaaaatgc ggatttcatc ctgcgactcg gcgtcgagaa
    19561 tcagtagccg cttggtgatg tccatggcca gttccatgtt gatctcacca tagatcaaga
    19621 tggtacggtt cttcagcagc ttctcttcca tcgagccgaa gccgtcgcgc ttgtcatccg
    19681 gcttgtcgtc gtcgtcctct tccggttcct catccgggtc aatgtccggg ggaaagtcat
    19741 catcgtcatc atcgtctagc atggccagtt cccatgggtg tgtcatccga tagcttctcc
    19801 ttgcggctgt accgcaatga tcgggcgagg cagaccagcg gcgcgccggt ctgcctcgtc
    19861 gtgttcaccg ttgccgtcct tagcggcgca ggttcgagaa cgccttcatg cccggataga
    19921 tggccgaatc gcccaggtct tcctcaatgc gcagaagctg gttgtacttg gcaatacggt
    19981 cgctgcgggc cggggcgccg gtcttgatct gtccggcgtt gagggccacg gccaggtcgg
    20041 cgatggtcgc gtcctcggtc tcgccgctgc ggtgggaggt gacggccgtc cagccgttgc
    20101 gttgcaccag gttgaccgat tcgatagcct cggtcagcgt gccgatctgg ttgactttgc
    20161 acagcaggct gttggctgcc ttgcgctcca gcgcctgctc aatgcgcttg acgttggtca
    20221 ccagcaggtc gtcgccgaca atctgcaaac gatcgcccaa ccgctcgacc atcagcgccc
    20281 agttgtccca gtcatcctcg gccaggccgt cctcgataga gataatcggg tacttgtcga
    20341 gccaccactc ccagaaatcg accatctcgc ggccggtcag cgccttgccc tcgaccttca
    20401 gatggtactt gccgtcctca tagaactccg acgcggccgg atccatg
//
LOCUS       DAOSMQ010000005        19747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_2742_length_19747_cov_5.036411, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000005 DAOSMQ010000000
VERSION     DAOSMQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 19747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19747
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_19747_cov_5.036411"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1957)
                     /locus_tag="PK829_00360"
     CDS             complement(152..1957)
                     /locus_tag="PK829_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU39699.1"
                     /translation="MSEQASSVRAAGSATWQLLGYLRPYRRQVSIAYVAMLLGTGLNL
                     IVPRVMAWAIDYGLAQRQASYLFLAGGIIMAIAVVRGVLGFAQRYYGEWLTHRVAYDL
                     RNDFYNAVQYQPFAFHDQSHTGDLMSRATGDIAESERFAGIGLMDFLSTMLLLAGVVV
                     AMLLQSVPLALLGLIPLPILLLTTTRFGLTIEPMFKRIQEQMGVLSNVMQESLTGIRV
                     VKAFAREPHELDKFDRENNEWFARREGVIKKWGNNWPFMTFLIAMSIFLLLLFGGPAA
                     LDGRITVGALFAMISYVLMLNTPVQRLGFLVNLAATAGASAARILEIVDLPGEVVDRP
                     DAIELGRARGDVRFDHVSFGYRDGLNILADIDFHARPGERVALIGPTGSGKSTITNLI
                     PRFYDVSSGRVLVDYQDVRDVQLRSLRRQIGIVMQEPFLFSQTVAENIAYGRPEASRE
                     EIEAAAHVAHAHDFILELQDGYETRVGERGVTLSGGQKQRIAIARALLADPRILILDD
                     STSSVDTETEHLIQSALAELMKGRTTFVIAQRLLTLKNADTILVLDQGRIAERGTHQS
                     LLAEGGLYRQIYDLQLKDQEEFVALQERMAAEARR"
     gene            2132..2329
                     /locus_tag="PK829_00365"
     CDS             2132..2329
                     /locus_tag="PK829_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39700.1"
                     /translation="MLLKRLLLAVISFGVAFGLTVLITMFIGTTPAEYGPIYMFFTTL
                     TIGLAIAIWLDKFMGTNLLPK"
     gene            2438..3325
                     /gene="folD"
                     /locus_tag="PK829_00370"
     CDS             2438..3325
                     /gene="folD"
                     /locus_tag="PK829_00370"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOU39701.1"
                     /translation="MTAAIIDGKAIAEKVRQEVRQAVARMKEDHGYTPGLATVLVGEN
                     PASATYVRSKRKTCEELGIVSFGHELPADATQQQVEKLVAELSANPNVNGILVQLPLP
                     KHLNEEAVLNSISLEKDVDGFHPVNIGRLAMKGRDPLFIPCTPYGCIVLLEESGIPIR
                     GADAVVVGRSNIVGLPVAMLLQKRDATVTICHSRTRDLPGHIRQADIVIAAIGQTEMI
                     TGDMLKPGAAVIDVGINQKEDPTAKRGYRLVGDVDFESAKEVAGAITPVPGGVGPMTI
                     AMLMKNTLRAAEIALAKRA"
     gene            3351..4343
                     /locus_tag="PK829_00375"
     CDS             3351..4343
                     /locus_tag="PK829_00375"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU39702.1"
                     /translation="MSGDAGEADFWRGRRVFITGCTGFLGTWLTAALLERGASVVGLV
                     REHEPESQLVRSGLIDRVEQVPGELLDYSLLQRTLIDCAIDTVFHLAGQTIVGVANRE
                     PVATFETNIRGTWLLLEAIRQTPTVRGVIIASSEKAYGEQLNLPYREDYSLQGRHPYE
                     VSKSCADLIAQSFAHTFGLAIAVTRCSNLYGGGDVSWNRLIPGTIRSLLHHERPVIRS
                     DGSFRRDYLYVADAVRGYLMLAERLTEPGVRGEPFNLGSGCPVSALDVVRTIIALSEH
                     PDWQPLILNEVKHEIQDEYLSPQKALAAVGWEPQYSLAAGLEEALRWYHAYLRP"
     gene            4340..5233
                     /locus_tag="PK829_00380"
     CDS             4340..5233
                     /locus_tag="PK829_00380"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU39703.1"
                     /translation="MSRPRVLVTGATGFVGRALLPHLVEQADVTLLLLEEFAGAALPA
                     PLGQLRPALDVVYSDLRNYPLTARAVRQARPERVIHLAAAGVSDPFLDPRLALSHNVA
                     GTLNLLRACFDGAAAIQQLIVARTSAELEPSNVYAASKAAAWAFGAMYARAAAWPIHG
                     AMIFQAYGPGQPPGMLLPAALRAARAGQDFPMTAGQQEKDWIHVDDVAAGLLALLGRD
                     LPPGATAELGTGQTTTVRAVVERLYALVGRGGRPLPGALPSRPGEAPRHAADAAATAA
                     LIGWRARVGLEEGLARLVEQE"
     gene            complement(5328..7010)
                     /locus_tag="PK829_00385"
     CDS             complement(5328..7010)
                     /locus_tag="PK829_00385"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOU39704.1"
                     /translation="MPITLATFRRNIDATILGRGQSYYDNGHVIGLDELDANRWQAEV
                     AGTYPYTVTIHIATDGALSWACSCPYVDGPVCKHVVATLYAIESALDPAAPQPRKSRQ
                     TRTDQVREAISQLTHDQLVALLVELANGDRQIAYMLLARYSSPTGDKLAAKRLVKDAL
                     SFGRDRYGLLDYRATIEAAQSVGRLFSRANKLREAGQVNEALLLYQAILEEVVPALQE
                     ADDSSGSLSGCVEMALEELEEVGAHLSPAGRVALFGYCLSDEMAKVMAEWEWRWALLQ
                     LAGRLMTTPLQRAELFDALDRTALRHDTDSWIREHDRTQAAEIKFAVIQQQDDEAAVQ
                     AFLEAHSEQEEMRIRLAHFYIDHGNPDAARQVCQVWLDDPPAHKLGRRRDFLEMLRLI
                     AERTGDKADIMRQTAALFLETGDMTIYQQLKEMVGPEDWRVYRPGLLARMETEGGLRL
                     NRGAVYAAEAMWAELMAVVRSHPDKAIVWHQQLAPHYPKELSAIYKQLAGRTLDQHMN
                     RAGYQNACAYLLRMQEVDPTGAAAVVAGWRAEYKNRRALQDELTRAFGPPFG"
     gene            7172..8014
                     /locus_tag="PK829_00390"
     CDS             7172..8014
                     /locus_tag="PK829_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOU39705.1"
                     /translation="MRWPHYEHVFFDCDSTLTAVEGIDVLAESVGKGQRVRILTDAAM
                     DGQLDLSEVYDKRLRAVRPTQQQVLDIRRSYKRHIVEDAARVVTALQGLGHKVYIISG
                     GLAEPVIEFGIYLGIPRERIRAVGLTYNELSGRWWEKGDERRYLAHQEGALTVSDGKA
                     QIVRELLGEQRGRSLLIGDGYSDLLAGAAVDLFVGYGGVVARPKVQAAAPAYITCPSL
                     APLLPLAGGPVALRLLRSWPMHYQTLSTKALYLIHTGVVTFTDEHLESRFRAAFLNPE
                     PTTH"
     gene            8091..9080
                     /locus_tag="PK829_00395"
     CDS             8091..9080
                     /locus_tag="PK829_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOU39706.1"
                     /translation="MPECLDLFGEILPKLAQVFFTRQTVLITASSGTGLWEAGVRNLV
                     NKKVLTCVNGAFAQRFSDVARLNGKATEVLDVPWGQPILPEQVAERLATGEFDAVTVV
                     HNETSTGVTSNIRDIARAVRALPNGSDIMILVDSVSGLAGARIEMDAWDLDMVLASSQ
                     KAFALPPGIAFCAVSDRAMAKAKTVPDRGYYFDFISLAKSIAKNQTPATPAVSLLYAL
                     NRQLDDILAEGMDNRFARHIQMRHQTLQWARHQGFDIFAAAGYESPTVTCVSNNREID
                     ISALNKFLRTRGMVISNGYGDLKGKTFRIAHMGDTQPAELETLFAAMDEFLTA"
     gene            9090..10676
                     /gene="serA"
                     /locus_tag="PK829_00400"
     CDS             9090..10676
                     /gene="serA"
                     /locus_tag="PK829_00400"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOU39707.1"
                     /translation="MYRILVSDKLGAAALERLKEHNDMSVDVKTGLSKEELLAVIPDY
                     DGLIVRSGTQVTADVLAAAKKLRVVGRAGVGVDNIDVKAATMSGVVVMNTPGANSMAT
                     AELTMALMLAVSRNIPQAHASLKAHEWRRSEFVGVQLYNKVLGIVGFGRVGKLVAERA
                     QAFGMEVLAFDPIALEETAREMGVMLVEMDELLAKSDYITLHAALNPSTSKLINAETI
                     AQMKDGVILINAARGKLLDEAAVAAALQSGKIKAAAVDVYSSEPPPADHPLIGLPNVV
                     HVPHLGASTHEAERDVGIQIVGQVVAALRGTDYSYAVNMPFELEGDYATVRPYLELAE
                     TLGRLHAGLADRPIRRIEVEVQGDVVGGLVRAIGAALLKGLLEKDADVAVNYINAPVL
                     AHDRNIAMTQTVGINSLDYPNLIASRAVCDNGERLLAGVLFGGAEPRIAQMDNYRLEA
                     RPEGVVLIMQNRDVPGVIGQVGTLLASYGLNIGEWRLGRNKPGGEALSFINLDAEPAG
                     EALRALTEIDAVTQAKVVRL"
     gene            complement(10763..11659)
                     /gene="jag"
                     /locus_tag="PK829_00405"
     CDS             complement(10763..11659)
                     /gene="jag"
                     /locus_tag="PK829_00405"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOU39708.1"
                     /translation="MSPSSTHEIESRGANVDAAIDAGLRRLGLARDQVTVTVIDEGSR
                     GLLGIGSREAIVRLSPVAAPPARPAAAPPPVARPAAPAPAARPEPARRAEPPAAPAPA
                     ARPEPARRAEPPAAPAPTPSAAAVPSAGEAEEVASADELAEEQQAAVAILSELLEKMH
                     VPASVSATQSEPDDLTGQRVNVIAIHGEDLTMLIGPRGETLDALQFLGRLMVAHELHR
                     RANFVVDVEGYRQRRVLALTRLAERMADKARQRGEPISLEPMTAYERRIIHMALRDAP
                     DVYTESAGEGKQRRVRIYPKAE"
     gene            complement(11665..12546)
                     /locus_tag="PK829_00410"
     CDS             complement(11665..12546)
                     /locus_tag="PK829_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOU39709.1"
                     /translation="MWDTLIVNPMINALLLFYNFLGQNFILAIAFLTILIRIITLPLN
                     LKQQRSMMHTQELQPEINAIQKKYRDNPQKMQEEFKRVGYNPADTLSGCIPTLIQFPI
                     LIGLYRAITTILGTTPQALFELVPRVYPAINLTNLIPVENKFMWLNLSQPDPIFVLPI
                     VVFVSMFIQQKLLTPAPPKGQTQQDNPTAGMSQSMMYTMPLMFGFFSLQFPAGLSIYF
                     ILANVIGIVQGLYLQRIMNRERAERAARGETRPYVVETEKSAAKSRQSANGAGESKTK
                     VTNNSTAPRRKKGSAKR"
     gene            complement(12561..13706)
                     /locus_tag="PK829_00415"
     CDS             complement(12561..13706)
                     /locus_tag="PK829_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU39710.1"
                     /translation="MSRPLARPALRLLLIAAALFLLTGCGSRLATTNWAGLSTDGRLV
                     YLAFGPQVLAYDPAAQAQAWIHPAASTGAAFFAAPAAADGRVVFGDYGHSGGLFSPRV
                     TVSVYALQEGDSAGASQLWVNTSDARDKIVAPALQVGEQVFLGTADNHILALDATSGR
                     LQWDYQTGHAIWGQPAFRDGTLYVASMDHSVYALDAATGELKWSQALGGALPSGPTLG
                     DGLLYVSSFDGNVHALDMATGEERWAAPSADNWVWGAPALLDGTLYFGDIGGTLYAVD
                     AATGEQKWTQETQNAIQTSPVVSNDTLYVASQVAAETPTGALTAYNVTDGTQLWSEPT
                     TTPLYATPVVVEDALVVSTQDANALLIGYDLANGQELWRYALPQTSN"
     gene            complement(13728..13937)
                     /gene="yidD"
                     /locus_tag="PK829_00420"
     CDS             complement(13728..13937)
                     /gene="yidD"
                     /locus_tag="PK829_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOU39711.1"
                     /translation="MKQIALLLIRLYQNTLSRVLPPSCRFTPSCSVYGYQAIEKYGFL
                     KGGWLALRRLSRCHPFNPGGYDPVP"
     gene            complement(13934..14320)
                     /gene="rnpA"
                     /locus_tag="PK829_00425"
     CDS             complement(13934..14320)
                     /gene="rnpA"
                     /locus_tag="PK829_00425"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOU39712.1"
                     /translation="MLPTSFRLRRSDDIARVRQRGRRWQHPLIVLFIDVQPQESSSVS
                     RFAFVAGRRIGKATVRNRAKRRLREIVRHHLETLEPGHDCLVVARPASASADYEALER
                     SVLELLTRAGLRRSDDCGRSPRELPL"
     gene            complement(14436..14567)
                     /gene="rpmH"
                     /locus_tag="PK829_00430"
                     /pseudo
     CDS             complement(14436..14567)
                     /gene="rpmH"
                     /locus_tag="PK829_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
     gene            complement(14753..16048)
                     /gene="murA"
                     /locus_tag="PK829_00435"
     CDS             complement(14753..16048)
                     /gene="murA"
                     /locus_tag="PK829_00435"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU39713.1"
                     /translation="MSKFIIEGGQPLHGTMTASGNKNAALKLLPACLLTDEPVILHNI
                     PAIRDVENTTLILSDLGAEVTPLGGGSWRVQAYNVVKTELDARLAGRTRASFVFAGPL
                     LARAGRVCLPIPGGDVIGGRPLDTHMQALKALGATVNMNRRGTFEIEAPQLRGAGYLL
                     LAEASVTATENAVMTAVLAPGETIIDNAACEPHVRDLCHFLNQMGANIEGIGSNRLQI
                     QGVRRLHGTEFRIGSDFMEVGSFIGAAAVTGGEVRIRDAQPRNLGMIRLVFERLGVRW
                     QDDGEDILVPGNQEMVIQEGLGGRIPEIKPMPWPGFPPDLMSIAVVIGSQANGSVLLH
                     DWMYESRFFFVDHLTFMGARVVLCDPHRVLVQGPSRLYANPAGVPSPDIRAGMAMVLA
                     ALCARGTSMIHNIQQVDRGYEHIDDKLRALGAQITRVEE"
     gene            16304..17107
                     /locus_tag="PK829_00440"
     CDS             16304..17107
                     /locus_tag="PK829_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU39714.1"
                     /translation="MPYLNANGSRLFYSDQGQADGLALLLLHGAAGSHLVWPGPLRQL
                     AGARVLALDLPGHGRSDPPGRRTIAHYAASVAAFVHALALPDVVIAGHSMGAAIALTI
                     ALEPPAALRGLALLGAAGRLRANEALLGGSLADFEAAADFIVEYGFADNGLAVPTAEL
                     RRKVRAAILSVGAMTTYGDFLACHRFDARAQLAGIDLPAWVIAGSADRLVPARAAEAL
                     AGGLPRGRLARLEGAGHFSMLERPDEVAALMAAFLAEIAGSLPGQRAPQ"
     gene            complement(17078..17704)
                     /gene="rdgB"
                     /locus_tag="PK829_00445"
     CDS             complement(17078..17704)
                     /gene="rdgB"
                     /locus_tag="PK829_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOU39715.1"
                     /translation="MINLPPRPPRLLVASHNRGKVAEYAELLGDLRVGWLTLDEAGVT
                     EDVPETEDTFRGNATLKATAYARQTGLLTLADDSGLEVDALGGQPGVWSARYGQPGFS
                     AVDRYRLLLRNLAGVPWEQRAARFHCVIALAAPDGEILGTADGVCEGLITFEPAGEGG
                     FGYDPVFLLPERGLTMAQLPAAEKNRLSHRARATAAIAPLLRRALAGE"
     gene            17793..18314
                     /locus_tag="PK829_00450"
     CDS             17793..18314
                     /locus_tag="PK829_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOU39716.1"
                     /translation="MKMSRLRFNFGFLLEADFGTIRRIELDYPSIKLSDDLTLTPLKG
                     VITATRTTEGIYIQGQLESAAVLECIRCLEETVVPLDFSLDELFFHPPQSAPPGENRV
                     GEDGMIDLAPLVRELSLLSLPIKVLCRPDCLGLCQECGANLNEGDCGCAEAAIDPRLA
                     ALQQLLNSASNEQ"
     gene            18329..19612
                     /gene="obgE"
                     /locus_tag="PK829_00455"
     CDS             18329..19612
                     /gene="obgE"
                     /locus_tag="PK829_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU39717.1"
                     /translation="MFYDEAKIHVRSGDGGDGMISFRREKYVRLGGPDGGDGGRGGDI
                     VFVATNHLNSLIHLQHQKHHRAGNGVHGTVQRMTGANGQDLRIEVPAGTIIRNAETGD
                     TLADLTTVGQEVVLLAGGRGGRGNIHFANSRDQAPRVAERGEPGEELWLRLELKLIAD
                     IGIVGVPNAGKSTLLSVVSAARPKIGDYPFTTLQPNLGVVALDDYETMVLADIPGLIE
                     GASAGVGLGHDFLRHIERTRVLIHLLDGMATDPLQDYAMINDELALYNPDLANKPQVV
                     VLNKIDLPDVQALEPLVREAISAAGHPFMAISAVTGEGIRPMLYEVKRLLAAAPVTAT
                     TAATTPAEGAPIVIRPRVEERAFTITREDGRVWRVSGKEIERVAAMTYFEFDDSLMRF
                     QTMLERLGITAALTEAGVRVGDTVFIGDQELEWGE"
BASE COUNT         3466 a         6597 c         6337 g         3347 t
ORIGIN      
        1 ggccttgccc agcagttcgt cgtcgtcgcg cagcagttgg cgaatgcgcg cctcgcgggc
       61 ctcgcgctcg gcgtcgatgt ttacgtcggt ggtggcgggc gcggcgtggc cgttggcccg
      121 ttcgtcggga ttcactttga gggccatgtc gctatctcct cgcctccgcg gccatgcgct
      181 cctggagagc gacaaactcc tcctggtctt tcagttgcag gtcgtagatc tgccggtaga
      241 ggccgccctc ggccagcagc gactggtgcg tgccgcgctc ggcgatgcgc ccctgatcga
      301 ggacgaggat ggtgtcggcg ttcttcagcg tcagcaggcg ctgggcgatg acgaacgtcg
      361 tccgcccctt catcagttcg gccagcgccg actggatgag gtgttccgtc tcggtgtcca
      421 cgctgctggt ggagtcgtcg aggatcagga tgcgcgggtc ggccagcagc gcgcgggcga
      481 tggcgatgcg ctgcttctgg ccgccgctca gcgtcacgcc gcgctcgccg acgcgcgtct
      541 cgtagccgtc ctgcagttcc aggatgaagt cgtgggcgtg ggccacatgg gccgcggcct
      601 cgatctcctc gcggctggcc tccggccggc cataggcgat gttctcggcc accgtctggc
      661 taaacaggaa cggctcctgc atgacgatgc cgatctggcg gcgcaggctc ctgagttgca
      721 cgtcgcgcac gtcctggtaa tcgaccagca cgcggccgct gctgacgtca tagaagcgcg
      781 ggataaggtt ggtgatggtt gacttgcccg accccgtcgg cccgatgagg gcgacgcgct
      841 cgcctggccg ggcgtggaag tcgatgtcgg ccaggatgtt caggccgtcg cgatagccga
      901 aggagacgtg gtcgaaccgg acatcgccac gcgcccggcc caattcgatg gcgtcgggcc
      961 ggtcaacgac ttcgccgggc agatcgacaa tttccaggat gcgcgcggcg ctggccccgg
     1021 ccgtggccgc caggttgacc aggaagccga gccgctggac gggcgtgttg agcatcagga
     1081 cgtaggagat catggcgaac agcgcgccga cggtgatgcg gccgtcgagc gctgccggcc
     1141 cgccgaagag cagcagcagg aagatgctca tggcgatcag gaaggtcatg aagggccagt
     1201 tgttgcccca cttcttgatg acgccctcgc gacgggcgaa ccactcgttg ttctcgcggt
     1261 cgaacttgtc cagctcgtgc ggctcgcggg cgaaggcctt gacgacgcgg atgccggtca
     1321 ggctctcctg catgacgttg gacaacacgc ccatttgctc ctgaatacgc ttgaacatcg
     1381 gctcgatggt caggccgaag cgcgttgtgg tgagcagcag gatgggcagc ggaatcagcc
     1441 ccagcaacgc cagcggcacc gactggagca gcatggcgac gaccacgccg gcgagcagca
     1501 acatcgtcga taggaagtcc atcaggccga tgccggcgaa gcgctccgat tcggcaatat
     1561 cgcccgtggc ccggctcatc aggtcgccag tgtggctctg gtcgtggaag gcgaagggct
     1621 ggtactgcac cgcgttgtag aagtcgttgc gcagatcgta ggccacgcga tgggtcagcc
     1681 actcgccgta gtagcgttgg gcgaagccaa ggacgccgcg cacaacggcg atagccatga
     1741 tgatgccgcc agcaaggaag agatacgacg cctgccgctg ggccaggcca tagtcgatgg
     1801 cccaggccat gacgcggggc acgatcaagt tcaggcccgt acccaacagc atggccacgt
     1861 aggcgatgct gacctggcgg cgataggggc gcaggtagcc cagcagctgc cacgtggcgc
     1921 tgccggccgc ccgcaccgaa ctcgcttgtt cactcatgat gcgcaaaacc cccttaaggt
     1981 cgttccatta taccacataa cctagagtac attaggcaaa tatctaacga actattgttt
     2041 ttgggcagcc cgcgctttgc ttgtcggcat ggtcttatct gctacaattg cctcggtttg
     2101 tgatggatta cacaactgga ggcaagcccg catgttgctc aaacgtctac tcttggcagt
     2161 catctccttc ggcgtggcct ttggtctgac agtgctgatc accatgttca tcggcacgac
     2221 cccggccgaa tatggcccca tctacatgtt cttcacgacg ctgacgattg gcctggccat
     2281 cgctatctgg ctcgacaagt tcatgggcac caacctgctg ccgaagtaag gggaacgcgg
     2341 gccgccaatt gattgccgat ccatgagcct ctgacatcag gtagaggctc tttctgtttc
     2401 ccacccagac tacacccaca ttgaggaaag atttaccatg accgcagcga tcattgacgg
     2461 aaaggccatc gccgagaagg tacgccagga agtgcgccag gccgtcgccc gcatgaagga
     2521 agaccacggc tacacgccgg ggctggcgac cgtgctggtc ggcgagaacc cggcctcggc
     2581 cacctacgtg cgcagcaagc gcaagacctg cgaggaattg ggcatcgtct cgtttggcca
     2641 cgaactgccg gccgacgcca cccagcagca ggtcgaaaag ctggtggccg aactgagcgc
     2701 caatcccaac gttaacggca tcctcgtgca gctgccgctg cccaagcacc tcaacgaaga
     2761 ggccgttctg aacagcatca gcctggagaa ggacgtcgac ggcttccacc cggtcaacat
     2821 cggccgcctg gccatgaagg gccgcgaccc gctgttcatc ccctgcacgc cctacggctg
     2881 catcgtcctg ctggaagaaa gcggcatccc catccgtggg gccgatgcgg tcgtcgtcgg
     2941 ccgctccaac atcgtcggtc tgcccgtggc catgctcttg cagaagcgcg atgccaccgt
     3001 caccatctgc cacagccgca cccgcgacct gcccggccac atccgccagg ccgacatcgt
     3061 catcgccgcc atcggccaaa ccgagatgat caccggcgat atgctcaagc ccggcgcggc
     3121 ggtcattgac gtgggcatca accagaagga ggacccaacg gccaagcgtg gctatcgcct
     3181 ggtcggcgac gtggatttcg agtcggccaa ggaagtcgcc ggggcgatca cgcccgtgcc
     3241 cggcggcgtc ggcccgatga ccattgccat gctgatgaaa aataccttgc gggcggccga
     3301 gatcgcgctg gccaaacggg cctagtcgcg acgggccggg gagacgccat ttgagcggcg
     3361 acgcaggcga agccgacttc tggcgcgggc gacgcgtctt cattaccggc tgcaccggct
     3421 tcctggggac ttggctcacg gcggcgctgc tggagcgcgg cgcgtcagtc gttggcctcg
     3481 tccgtgagca cgagccggag tcgcaactcg tccgcagtgg gctgatcgac cgcgtcgagc
     3541 aggtgccggg cgagttgctg gattacagcc tcctccagcg cacgctcatc gactgcgcca
     3601 tcgacacggt ttttcacctg gccggacaga cgatcgtcgg cgtggccaac cgcgaaccgg
     3661 tagccacatt cgagaccaac atccgcggca cgtggctgct actggaggcc atccgccaga
     3721 cacccacggt gcgcggcgtc atcatcgcca gcagcgaaaa ggcctacggc gagcaactca
     3781 acctgcccta ccgcgaagat tactcgctgc agggccgcca cccctacgag gtctccaaga
     3841 gctgcgccga tctgatcgct caatcgttcg cccacacctt cggcctggcc atcgccgtca
     3901 cgcgctgctc caacctctac ggcggcggcg atgtgagctg gaaccggctg attccgggaa
     3961 ccatccgcag cctcctgcac cacgagcgcc cggtcatccg ctccgatggc tccttccggc
     4021 gcgactacct ctacgtcgcc gacgccgtgc gcggctacct gatgctggcc gagcggctga
     4081 ccgagccagg cgtgcgcggc gagccgttca atctcggctc cggctgcccc gtctccgccc
     4141 tcgacgtcgt gcggacgatc atcgccctgt cggagcaccc cgactggcag ccgctcatcc
     4201 tgaatgaggt caagcacgag attcaggacg agtatcttag cccgcagaag gcgctggcgg
     4261 cggtaggctg ggagccgcag tacagccttg ccgccggcct ggaagaggcc ctgcgctggt
     4321 accacgccta cctgcgccca tgagccggcc gcgcgtcctc gtcaccggtg cgaccggctt
     4381 cgttggccgc gcgctgctgc cacatctggt cgagcaggcc gacgtgacgc tgctgctgct
     4441 ggaggaattc gccggcgcgg ccctgcccgc gccgctggga cagttgcggc cggcattgga
     4501 tgtcgtctac agcgacctgc gcaactatcc cctgaccgcc cgcgccgtgc gccaggcccg
     4561 gccggagcgg gtcatccacc tggccgcagc cggcgtcagc gacccattcc tcgacccgcg
     4621 cctggccctc agccacaacg tcgccggcac gctcaacctg ctgcgcgcct gcttcgacgg
     4681 cgcggcggcg atacagcaac tcatcgtcgc ccgcacctcg gccgagctgg aaccgtcgaa
     4741 cgtctacgcc gccagcaagg ccgccgcgtg ggccttcggc gcgatgtacg cccgcgccgc
     4801 cgcctggccc atccacgggg cgatgatctt ccaggcctat ggccccggcc agccgccggg
     4861 gatgctgctg ccggccgccc tgcgcgccgc ccgcgccggc caggacttcc ccatgaccgc
     4921 gggccagcag gagaaggact ggattcacgt cgatgacgtg gccgccgggc tgctggcgct
     4981 gctgggccgc gacctgccac cgggagcgac ggccgaactg ggcacagggc agacaacgac
     5041 cgtccgcgcg gtcgtggagc ggctctatgc gctggttggc cgcggcgggc ggccgctgcc
     5101 cggcgcgctg ccctcccgcc ctggcgaggc cccccgccac gccgccgacg ccgccgccac
     5161 cgcggcgctc atcggctggc gggcgcgggt ggggttagag gagggactgg cgcgattggt
     5221 ggaacaggaa tgagaaaaac acgggtgacg tcagatgaag gcggtttgct gaattatatt
     5281 tacaccccat ttaagaccct ccgccccccg atcgacatca cccaaaatca cccaaacggc
     5341 gggccgaagg ctctggttaa ctcatcctgc agggcgcggc gattcttata ctccgcccgc
     5401 caaccggcca ctaccgccgc tgcgccggtc gggtctacct cctgcattcg cagtagatag
     5461 gcgcacgcgt tctgatagcc agcgcgattc atgtgctgat ccagcgtccg cccggccaat
     5521 tgcttataga tagcgctcaa ctccttgggg tagtgcgggg ccaactgttg atgccagacg
     5581 attgccttgt cagggtggct gcgaacgacg gccatcagct ccgcccacat cgcctccgca
     5641 gcatagacag cgccccgatt gagccgcagc ccgccctcgg tctccatgcg cgccagtagg
     5701 ccgggcctgt agacgcgcca atcctccggc ccgaccatct ccttgagctg ctggtagatg
     5761 gtcatatcgc cggtctccag gaacagcgcg gcggtctggc gcatgatgtc ggccttgtct
     5821 cctgtccgct ctgctatcag gcggagcatc tccaaaaaat cgcgccgccg ccccaactta
     5881 tgagcgggcg gatcgtcgag ccacacctga cacacctgcc gcgccgcatc cgggttgccg
     5941 tgatcgatgt aaaaatgagc cagccgaatg cgcatctcct cttgctcact atgagcttcc
     6001 agaaaggctt gcacggctgc ctcgtcatct tgctgctgga taacggcgaa cttgatctca
     6061 gctgcctggg tgcggtcgtg ctcgcgaatc catgaatccg tgtcatggcg cagagctgtc
     6121 cggtctagcg catcgaaaag ctctgcccgc tgcaacgggg tggtcataag ccggccggcc
     6181 agttgcagca aggcccaccg ccactcccat tcggccatta ccttggccat ctcatcgcta
     6241 aggcagtagc caaacaaggc cacgcgtccg gcgggcgaca agtgcgcccc gacctcctcc
     6301 agctcctcca gcgccatctc aacgcaaccg ctcagcgacc cagatgaatc atcggcttcc
     6361 tgcagcgcgg gcacgacctc ttcgaggata gcctgataga gcagcagggc ttcattgacc
     6421 tgcccggcct cgcgcaactt gttggcgcgg ctgaacagcc ggccgactga ctgcgccgct
     6481 tctatcgtgg cgcgatagtc gagaaggccg taccgatcgc ggccgaagct gagggcgtcc
     6541 ttgaccaggc gcttggcggc cagcttgtcg cctgtgggtg aactgtagcg ggccagcagc
     6601 atgtaggcga tctggcgatc cccattcgcc agttcgacga gcaaagcaac cagttggtca
     6661 tgggtcaatt ggctgatagc ttcgcgcact tgatcggtgc gggtctggcg acttttgcgc
     6721 ggctggggag cggccggatc gagcgcgctt tcgatggcat agagcgtggc gacgacgtgc
     6781 ttgcagacag ggccgtcaac gtaggggcag gaacaagccc aactaagtgc gccgtcagta
     6841 gcgatgtgga tcgtgacggt atagggatag gtcccggcca cttccgcttg ccaacggttg
     6901 gcgtctagct catccagccc gatcacatgg ccgttgtcat aatagctttg cccgcggccg
     6961 agtatggtcg catcgatgtt gcgtctgaag gtggcgagag tgatgggcat gggtatggcc
     7021 tcgcaggcag cttagcctga ttatagcgcg tccgcgcaat ccgttggcaa aaaccagcgt
     7081 tagtgactaa aatcacgagt cggcacacca ggtgccccgc tgacaccgcg tcccgacagc
     7141 cgccggcacg ccaaccggac cgataatgcc aatgcgctgg ccccactatg agcacgtctt
     7201 cttcgactgc gactccacgc tgaccgctgt ggaaggcatc gacgtgctgg ccgaatcggt
     7261 cggcaagggg cagcgcgtcc gaattctgac cgacgcggcc atggacggcc agctcgacct
     7321 gagcgaagtg tatgacaagc gcctgcgcgc cgtgcgcccg acccagcagc aagtcctcga
     7381 catccgccgc agctacaagc gccacattgt cgaagacgcc gcccgcgtcg tcaccgccct
     7441 gcaaggactg ggccataagg tctatatcat cagcggcggc ctggccgagc cggtcatcga
     7501 gtttggcatc tacctgggca tcccccgcga acgcattcgc gctgtgggcc tgacatacaa
     7561 cgaattgtcc ggccgctggt gggagaaagg ggacgagcgc cgctacctgg cccaccagga
     7621 aggtgcgctg acggtcagcg acggcaaggc gcagatcgtc cgcgaactgc tgggcgagca
     7681 acgcggccgc tcgctgctga ttggcgacgg ctacagcgat ctcctggccg gcgcagcggt
     7741 cgatctcttt gtcggctacg gcggcgtggt cgcgcggccg aaagtgcagg ccgccgcgcc
     7801 cgcctacatc acttgcccca gtctggcccc gctgctgcca ttggccggcg ggccggtcgc
     7861 gctgcgcctc ctgcgcagct ggccgatgca ctaccaaacc ctgagcacga aagccctata
     7921 cctgatccat acaggagttg tgacatttac cgatgaacac cttgaatcca gattccgggc
     7981 agccttcctc aacccggagc caacaacaca ttaaactgtt cattcctgga ccgacagagg
     8041 tccgccccga aatccttgac gcccaaacgc aatggatggt cggccatcgc atgccggagt
     8101 gcctggacct ctttggcgaa atcttgccca agctggccca agttttcttc acccggcaga
     8161 ccgtcctcat caccgcctcg tcgggcacgg gcctgtggga agcgggggtg cgcaacctcg
     8221 tcaataagaa agtcctgacc tgcgtcaacg gcgcgttcgc ccagcgcttc agcgacgtcg
     8281 cccgcctgaa cggcaaggcg acggaggtgc tcgacgtgcc ctggggccaa cctatcctgc
     8341 cggagcaggt cgctgagcgg ctggccacag gcgaatttga tgccgtgacc gtcgtccaca
     8401 acgagaccag caccggtgtc accagcaaca tccgcgacat cgcccgcgcc gtccgcgccc
     8461 tgcccaacgg gagcgacatc atgatcctgg tggactccgt cagcggcctg gccggagcgc
     8521 gcatcgaaat ggacgcctgg gacctggaca tggtgctggc ctccagccaa aaggccttcg
     8581 ccctgccgcc gggcattgcc ttctgtgctg tgtccgaccg ggccatggct aaggccaaga
     8641 ctgtgcccga ccgcggctac tacttcgact tcatcagcct ggccaagtcg attgccaaga
     8701 accagacgcc ggccactccg gccgtgtccc tgctctacgc cctcaaccgc cagctggacg
     8761 acatcctggc cgaaggcatg gacaaccgct tcgcccgcca catacagatg cgccaccaga
     8821 cgctgcaatg ggcgcgccac cagggcttcg acatcttcgc cgccgccggc tacgagtcgc
     8881 ccacggtcac ctgcgtcagc aacaaccgcg agatcgacat ctcggccctg aacaagttcc
     8941 tacgcacgcg cggcatggtc atctccaacg gctacggcga tctgaagggc aagaccttcc
     9001 gcatcgccca catgggcgat acccagccgg ccgaactgga aacactgttc gccgcaatgg
     9061 acgaattcct taccgcttag gggtacacta tgtatcgcat actcgtttcc gacaaactcg
     9121 gcgcggcggc cctggagcgc ctgaaagaac acaacgacat gtccgtcgac gtgaagacgg
     9181 ggctgagcaa ggaagaactc ctggccgtca tccccgacta cgacgggctg atcgtccgta
     9241 gcggcacgca ggtgacggcc gacgtgctgg ccgccgccaa gaagctgcgc gtcgtcggcc
     9301 gcgccggtgt gggcgtcgat aacatcgatg tcaaggcggc cacgatgagc ggcgtcgtgg
     9361 tcatgaacac gcccggcgcg aacagcatgg cgacggccga actgaccatg gccctcatgc
     9421 tggctgtcag ccgtaacatt ccccaggccc acgcctcgtt gaaggcccat gagtggcgac
     9481 gcagcgagtt tgtcggcgtc cagctctata ataaggtgtt gggcatcgtc ggcttcggcc
     9541 gcgtcggcaa gctcgtggcc gagcgggcgc aggccttcgg catggaggtg ctggcctttg
     9601 accctatcgc cctggaggag acggcgcgcg agatgggcgt tatgcttgtg gagatggatg
     9661 agctgctggc caagtccgac tacatcaccc tccacgccgc gctcaacccg tcgacgtcca
     9721 agctcatcaa cgccgagacg atcgcccaga tgaaggacgg cgtcatcctc atcaatgccg
     9781 cgcgcggcaa gctgttggac gaagccgccg tggccgcggc gctgcaaagc ggcaagatca
     9841 aggccgccgc tgtggatgtc tactcgtccg agccgccgcc ggccgaccat ccgctcatcg
     9901 gtctgcccaa cgtcgtccac gtgccccacc tgggcgccag tacccacgag gccgagcgcg
     9961 acgtcggcat ccagatcgtc ggccaggttg tcgccgcgct gcgcggcacg gactacagct
    10021 atgccgtcaa tatgcccttc gaactggagg gcgattacgc caccgtgcgc ccctacctgg
    10081 aactggccga gacgctcggt cgcctgcatg ccggcctggc cgaccggccc atccgccgca
    10141 tcgaagtcga ggtgcagggg gatgtcgttg gcggcctggt gcgcgccatt ggcgcggcgc
    10201 tgctgaaggg gctgctggag aaggacgccg atgtcgccgt caactacatc aacgcgccgg
    10261 tgctggccca cgaccgcaac atcgccatga cacagacggt gggcatcaac agtcttgact
    10321 accccaacct catcgcttcg cgggccgtct gtgacaacgg cgagcggctg ctggccggcg
    10381 tccttttcgg cggcgcggag ccgcgcatcg cccagatgga caactaccgg ctggaggccc
    10441 gccccgaggg cgttgtgctg atcatgcaga accgcgacgt gcccggcgtc atcggccagg
    10501 tgggcacgct gctggcgtcc tatgggttga acatcggcga gtggcggctg ggccgcaaca
    10561 agcctggcgg cgaagcccta tcgttcatca acctggatgc cgaaccggcc ggagaggccc
    10621 tgcgcgctct gaccgagatc gatgccgtga cccaggccaa ggtcgtccgt ctctagccgg
    10681 cgacgcgaca gaagcaggaa agcgaaaggc cggagcgtca tctgctccgg cctttgtcgt
    10741 tcgggggtga agccgccggc ggctattccg ccttggggta gatgcgcacg cggcgctgct
    10801 tgccctcgcc ggcgctctcg gtatagacgt cgggcgcgtc acgcagggcc atgtggatga
    10861 tgcgccgctc ataggcggtc atcggctcca ggctgatcgg ttcgccgcgt tggcgggcct
    10921 tgtcggccat acgctcggcc agccgcgtca gcgccagcac gcgccgctgc cggtagccct
    10981 cgacatcgac gacgaaattc gcccgccggt gaagctcgtg ggccaccatc agacggccca
    11041 ggaattggag cgcgtcgagt gtctcgcccc gcgggccgat gagcatagtc aggtcttcgc
    11101 catgaatggc aataacgttg acgcgctggc cggtgaggtc gtcgggttcc gactgcgtcg
    11161 ccgacacgct ggccggcacg tgcatcttct ccagcaattc cgacaggata gccacagccg
    11221 cttgctgctc ctcggccaac tcgtcggccg aagcgacctc ctcggcttcg ccggcgctgg
    11281 ggacggccgc ggccgaaggc gtcggggcgg gcgcagccgg tggttcggct cgcctggccg
    11341 gctcgggacg ggccgctggg gcgggcgcag ccggtggttc ggcgcgcctg gccggctcgg
    11401 gacgggccgc cggggcgggc gcagccgggc gggccacagg cggcggagcc gcggccgggc
    11461 gggcgggcgg ggcggccacg ggcgacaggc gtacgatggc ttcgcgactg ccaatgccca
    11521 gcaggccgcg gctgccttcg tcaataaccg tcacggtcac ctggtcgcgc gccaggccca
    11581 ggcggcgcag gccggcgtcg atggccgcgt ccacgttcgc gccgcgcgat tcaatctcat
    11641 gagtactgct tggtgacata tcgcctaccg ttttgcggaa cccttcttcc gccggggagc
    11701 cgtcgagttg ttcgtgacct tggtcttgct ctcgccagcg ccattggccg actggcggct
    11761 cttggcggca ctcttctctg tctcaacgac atacgggcgc gtctcgccac gggcggcgcg
    11821 ctcggcgcgc tcgcggttca tgatccgttg caggtacagg ccctggacga taccgatgac
    11881 gttggccaga atgaagtaga tcgacaggcc ggccgggaac tggagcgaga agaagccgaa
    11941 catcagcggc atggtgtaca tcatcgattg cgacatgcct gcggtggggt tgtcctgttg
    12001 cgtctgcccc ttcggcggcg ccggcgtcag caacttctgt tggataaaca tgctgacaaa
    12061 gacgacaatc ggcaggacga agatcgggtc cggctggctc agattgagcc acatgaactt
    12121 gttctcgacc gggatgaggt tcgtcaggtt gatggccggg tagacgcggg gcacaagctc
    12181 aaacagggct tgcggcgtcg tgcccagaat cgtggtgatg gcccgataga ggccgatcag
    12241 gatagggaac tggatcaggg tcgggatgca gccgctgagc gtatcggccg gattgtagcc
    12301 aacgcgcttg aactcttcct gcatcttctg cgggttatcg cggtacttct tctgaatcgc
    12361 gttgatctcc ggctggagtt cctgcgtgtg catcatcgag cgctgttgct tcaggttcag
    12421 cggcaaggtg atgatgcgga taaggatcgt caggaaggcg atcgccaaga tgaagttctg
    12481 ccccaggaag ttgtagaaca acaacagggc gttgatcatg gggttaacga tcagggtatc
    12541 ccacatggtg tcctcgcgcg ctagttagac gtctggggca gcgcatagcg ccagagttcc
    12601 tggccgttgg ccagatcata gccgataagc aaggcgttgg cgtcctgcgt ggagacaacc
    12661 aaggcgtcct cgaccaccac cggcgtagcg tacagcggcg tggtcgtcgg ttccgaccag
    12721 agctgcgtgc cgtcagtgac gttgtaggct gtcaaggctc cggttggcgt ctccgcggcc
    12781 acctgggagg cgacgtagag cgtatcattg ctgaccaccg ggctggtctg aatcgcgttc
    12841 tgcgtctctt gcgtccactt ctgctcgccg gtggcggcat ccacagcata gagcgtgcca
    12901 ccgatgtcgc cgaagtagag cgtgccgtcc aatagcgccg gcgcgcccca aacccagttg
    12961 tcggcgctgg gcgcggccca gcgctcttcg ccagtagcca tgtcaagcgc atggacgttg
    13021 ccatcgaagc tggagacgta gagcaggcca tcgcccaggg tcgggccgct gggcagcgcc
    13081 ccgcccaacg cctggctcca cttcaactcg cccgtggccg catcgagagc gtagacggag
    13141 tggtccatgg aggccacgta cagcgtgcca tcccggaagg caggctggcc ccagatggcg
    13201 tgccccgtct ggtaatccca ttgcaggcgg ccgctcgtcg catcgagggc cagaatatgg
    13261 ttgtcggctg tgcccagaaa gacctgctcg cccacttgca gcgccggagc gacgatcttg
    13321 tcgcgcgcgt cgctggtgtt gacccacaat tgggacgcgc cggcgctatc gccctcctgc
    13381 agggcataga cggacacagt gacgcggggg gagaagagac cgcctgagtg gccatagtcg
    13441 ccgaagacaa cccgaccatc ggccgcggcc ggagccgcaa agaaggccgc gccggtagac
    13501 gcggccgggt gaatccaggc ctgtgcttgc gccgccggat cgtaggccag gacttgcggc
    13561 ccgaacgcca gatagaccag ccgtccgtcc gtagaaagcc cggcccagtt ggtagtggcc
    13621 agccggctac cgcagccggt cagcagaaag agagcggccg cgattagcag cagccgcagt
    13681 gcgggccgag cgagcggccg actcatgtgg tccaatgaac acctccgtca aggtaccggg
    13741 tcatagccgc cgggattgaa cgggtgacaa cggctgaggc gccgcagcgc cagccagccc
    13801 cccttcagaa acccatactt ctcaatcgcc tgataaccat acacagagca cgaaggcgta
    13861 aaccggcaac tcggcggaag cactcgagag agtgtgtttt ggtatagtcg aatgagcagc
    13921 aaggcgatct gtttcacagc ggtaactccc gtggcgatcg cccgcaatcg tcagaccgtc
    13981 gcaagccggc gcgcgtcagc aactccagaa cgcttcgttc caacgcctca tagtcggccg
    14041 aggccgacgc cgggcgggcc accaccaaac agtcgtggcc cggttcgagt gtctccagat
    14101 gatgacgcac gatctctcgc aaccttcgct tagcccgatt acgcaccgtg gctttgccga
    14161 ttcggcgccc ggcgacaaag gcgaagcgac tgacagacga cgattcctgc ggctgaacgt
    14221 cgataaacaa tacgatcaaa gggtgctgcc agcgccgccc tcgttgacga acgcgcgcaa
    14281 tatcgtctga gcggcgcagt cgaaaggacg tcggcagcat ggaacattac aatacgcgcg
    14341 ccgtctcagc cgccgccgtg gcgcttgacg gagcgatagg ccgtggccga cgtgccgttc
    14401 cagttcacct gcttgctgtg gttgttcata gacaccgaca gcacacggcg accccggcgg
    14461 cgacggcgct ggagcacatc gcgaccacct ttggtcgaca tgcgctcgcg gaagccatgc
    14521 acacgcatcc ggcggcgaat cttcggctga tatgtacgtt tcgtcatgat gcactctcca
    14581 aaaaaacaag ggcgcgttgc cctgtcattg cgatccggtt attcgtagcg cataggggac
    14641 gtctgcacgg cagaccgtca cgacaaaccc ataatggccc gtcgatgcaa tacttcatta
    14701 tagcggcaga cctgtctttt gcaacaggcg gccgcccgtt ttcacgcctt ccctactctt
    14761 ctacccgcgt gatctgcgct cccaaggccc tcagcttgtc gtcaatgtgc tcatagccgc
    14821 gatcaacctg ctgaatattg tggatcatgc tcgtgccgcg ggcacacagg gccgccagca
    14881 ccatggccat gccagcgcgg atgtccgggc tgggcacgcc ggcggggttg gcgtagagcc
    14941 ggcttggccc ctgcaccagc acacggtggg ggtcgcacag caccacccgc gcgcccatga
    15001 acgtcaggtg atccacaaag aaaaagcgac tctcgtacat ccagtcgtgg agcaagacgg
    15061 agccgttagc ctgtgagccg atgacgacgg cgatactcat cagatcgggc ggaaagccgg
    15121 gccagggcat gggcttgatc tccgggatgc gcccgcccaa cccttcctgg atgaccatct
    15181 cctggttgcc aggcacaagg atgtcctcac catcgtcttg ccagcgcacg cccaggcgct
    15241 cgaagaccag acgaatcatg ccgaggttgc gcggctgggc gtcgcgtatg cgcacttcac
    15301 ccccggtcac ggccgccgcg ccgataaagc tgccgacctc cataaagtcg gagccgatgc
    15361 gaaattccgt gccgtgcagc cgcctgacgc cttgaatctg caagcggttg ctgccaatgc
    15421 cctcgatgtt ggctcccatc tggttgagga agtggcacag gtcgcgcacg tgtggctcac
    15481 aggcggcgtt gtcgataatc gtctcgccgg gggcgaggac ggcggtcatg acggcattct
    15541 cggtggctgt cacgctggcc tcagccagca gcagatagcc cgcgccgcgc agttgcggcg
    15601 cttcgatctc aaacgtcccg cgccggttca tgttgaccgt cgcgcccagg gccttcagag
    15661 cctgcatatg cgtgtccagc ggccggccgc cgatgacgtc gccgccgggg atgggcagac
    15721 acacccgtcc ggcgcgggcc aacagcggcc cggcaaagac aaacgaagcc cgcgtgcgcc
    15781 ccgccagccg ggcatccagt tccgtcttga ccacgttata agcctggacg cgccagctgc
    15841 cgccgcccag cggcgtcacc tcagcgccca gatcgcttag gatgagggtg gtgttctcca
    15901 cgtcgcggat ggcgggaatg ttgtgcagaa tcaccggctc gtccgtcaac aggcaggccg
    15961 gcaggagttt gagggcggca ttcttgttgc cgctggccgt catcgtgccg tgcagcggct
    16021 gccctccttc gatgatgaat ttgctcatgg ttgggttccg gccgggcggc gttttcccgg
    16081 cttgcggccc attgtaccaa tcggcgcggc gcaggccact tggcaccagg gcattgccaa
    16141 ggtcacccca ggcgcgccga cctgacaggt gagcggccac gaatcgtact tgcaccagag
    16201 aacgactggc cgctcacctg tcaggtcaat ggtcgctaga cgactttgga atagccctgc
    16261 acttggcact gtcgttgtcg ccggcatggg ctacaatggc ggcatgccct acctgaacgc
    16321 caatggcagc cggctcttct atagcgacca gggccaggcc gacggcctgg ccctgcttct
    16381 gttgcatggc gcggccggct cacacctggt ctggcctggc ccgctgcggc agctggccgg
    16441 ggcgcgcgtg ctggccctcg acctgcccgg ccacggccgc tccgacccac ccggccgccg
    16501 caccattgcc cactacgccg catcggtcgc ggcctttgtc cacgcactcg ccttacccga
    16561 tgtggtcatc gccggccact cgatgggcgc ggctattgcc ctgaccatcg ccctggagcc
    16621 gccggcggcg ttgcgcgggc tggcgctgct gggcgcggcg gggcggctgc gcgccaacga
    16681 ggccctgctg ggcggcagcc tggccgactt cgaggccgcg gccgatttca tcgtcgagta
    16741 cggcttcgcc gacaacggcc tggccgttcc cacggccgaa ctgcggcgca aggtgcgcgc
    16801 ggccatcctg agcgtcgggg cgatgactac ctatggcgac ttcctggcct gccaccgctt
    16861 cgacgcgcgg gcgcaactgg ccggtatcga cttgcctgca tgggtcattg ccggctccgc
    16921 cgaccggctc gtgccggcgc gcgcggccga agcgctggcc ggcggcctgc cccgcggccg
    16981 gctggcgcgg ctggagggcg cgggccactt cagtatgctg gagcggcccg acgaggtcgc
    17041 cgcgctaatg gccgccttcc tggccgagat agccggctca ctccccggcc agcgcgcgcc
    17101 gcagtagcgg ggcgatggcc gccgtggccc gggcgcggtg actaaggcgg ttcttttcgg
    17161 ccgccggcag ctgggccatc gtcagcccac gctcaggcag cagaaagacc gggtcatagc
    17221 cgaagccgcc ttcgccggcc ggctcgaaag tgatcagccc ttcgcagacg ccgtcggccg
    17281 tgcccaggat ttcgccgtcc ggcgcggcca gggcgatgac acagtggaag cgggcggcgc
    17341 gctgctccca gggcacgccg gccaggttac gcagcaacag ccggtagcga tcgaccgcgg
    17401 agaagcccgg ctggccatag cgcgccgacc acacgcctgg ctgtcccccc agcgcgtcca
    17461 cttccagccc ggagtcatcg gccagggtca gcaagccggt ctgccgggcg taggccgtgg
    17521 ccttcagcgt ggcgttgccg cgaaaggtgt cctccgtttc gggcacgtcc tcggtcacgc
    17581 cggcctcgtc cagcgtcagc cagcccacgc gcaggtcgcc cagtagctcg gcgtattcgg
    17641 cgactttgcc ccggttgtgc gaggcgacca ggagccgcgg cggtcttggt ggtaggttaa
    17701 tcactgctaa caccctccgg tataacccgc gccagccagg cgcgggtgtg gtatactccc
    17761 attctatccc gtaccaagtc ggaaggttta ccatgaagat gtcccgtttg cgcttcaatt
    17821 tcggtttcct gctggaggcc gacttcggga cgatccgtcg tatcgaactg gattatccca
    17881 gcatcaagct gagcgatgat ctgacgctaa cccccctcaa gggcgtcatc accgccaccc
    17941 gaaccaccga aggcatctac attcaggggc aactggagag cgctgccgtc ctggaatgta
    18001 tccgttgtct ggaggaaaca gtcgtccctc tggacttcag cctcgatgaa ctattcttcc
    18061 acccgcccca gagcgcgccg ccgggcgaga accgcgttgg cgaggatggc atgatcgatc
    18121 tcgccccgct ggtgcgtgaa ttgagcctgc taagcctgcc aatcaaggtg ttgtgccggc
    18181 ccgactgcct gggcttgtgc caggagtgcg gcgcgaacct caacgagggc gattgtggct
    18241 gcgccgaggc agcaatcgat ccgcgtctgg cggcgctaca gcagttgctc aacagcgcca
    18301 gtaacgaaca ataagcaccg gatagcctat gttttacgat gaggccaaaa tccacgtccg
    18361 cagcggcgac ggcggcgacg gcatgatcag tttccgccgc gagaaatacg tgcggctggg
    18421 tgggccagac ggcggcgacg gcggccgcgg cggcgatatt gtctttgtgg ccaccaacca
    18481 cctcaatagc ctgatccacc tgcagcacca gaagcaccac cgtgccggca acggcgtcca
    18541 cggcacggtg caacgtatga ccggggccaa cggccaagac ctgcgcatcg aagtgcccgc
    18601 gggtacgatc atccgcaacg cggagacggg cgacaccctg gccgacctga ccacggtcgg
    18661 gcaggaagtt gtgctgctgg ccggcggccg gggcggacgg ggcaatatcc acttcgccaa
    18721 cagccgcgac caggccccgc gcgtggccga gcgcggcgag ccgggcgagg agttgtggct
    18781 gcggctggaa ctaaagctca ttgccgacat cggcatcgtc ggcgtgccca acgcgggcaa
    18841 atcgacgctg ctgtccgttg tcagcgccgc gcggccgaag atcggcgact acccctttac
    18901 cactctacag cccaacctcg gcgtcgtcgc tctcgacgac tacgagacga tggtgctggc
    18961 cgacattcca gggctgatcg aaggcgcgtc ggccggcgtg gggctgggtc acgacttcct
    19021 gcgccacatc gagcgcacgc gcgtgcttat ccacttgctg gacggcatgg ccaccgaccc
    19081 actacaagac tacgccatga tcaacgacga gctggccctc tacaatcccg atctggccaa
    19141 caagccgcaa gtggtcgttc tcaacaagat cgatttgccc gacgtgcagg cgctggagcc
    19201 gctggtgcgc gaggccatca gcgccgccgg tcaccccttc atggctatct cggcggtgac
    19261 cggcgagggc atccggccga tgctctacga ggtcaagcgc ctgttggcgg ccgcgccggt
    19321 gaccgcgacc acggccgcga ccacaccggc cgagggcgcg ccgatcgtca ttcgtccgcg
    19381 agtcgaagag cgcgccttca cgatcacgcg tgaggacggc cgcgtctggc gcgtcagcgg
    19441 caaagagatc gagcgcgtcg ccgccatgac ctacttcgag ttcgacgatt cactgatgcg
    19501 ctttcaaacg atgctggagc gcctgggcat caccgccgcg ttgaccgagg cgggcgtccg
    19561 ggtaggcgac actgtcttca tcggcgatca agaactggag tggggcgaat agctccccgg
    19621 cgcagcccca tagccagcca tgaccgacag cgatccccag gcactcctgc gcgccgccat
    19681 tctggacgag gagcgctttg tcgaggccgt cttccagggc gcgcggccgg gcatcgcgtc
    19741 gccgtgg
//
LOCUS       DAOSMQ010000006        15988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_3690_length_15988_cov_3.847424, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000006 DAOSMQ010000000
VERSION     DAOSMQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15988
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3690_length_15988_cov_3.847424"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1191
                     /locus_tag="PK829_00460"
     CDS             91..1191
                     /locus_tag="PK829_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOU39718.1"
                     /translation="MTRTNTLLPISERPVPKGPIWLDEEAFLREAGAQSLKTETTYRS
                     GLRLFADWLQHFRRAGYAKSDAWPLRPDHLTTDVILEYRNWLLANRSRATVTTYMAAV
                     AGYLNFLDGYDRLPDTVQLGKLQRQMARRQIERNQAEAVIDLDIARQDIPRIVAYYDG
                     LPLPPQNDVYNRRLSLLRDRAIVKMLYSTAARISEVVSLNRANVGHGRAAYATIVGKG
                     NKGRTLHIREYARQAVVAYLAERTDGNSALFVSHSRNSTSARLSITSVHKVVKRAVHA
                     LHLHESLSAHDFRHYRATQLLRAGMPLEVVQEFLGHADVTTTRNIYAPVLGVQVVTEW
                     LDNLDVPPKEALSDVNGPLVTDERLLSMLDDD"
     assembly_gap    1381..1390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1391..2235)
                     /locus_tag="PK829_00465"
     CDS             complement(<1391..2235)
                     /locus_tag="PK829_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39719.1"
                     /translation="MALSGQSVGGLEDVTLRVRAAIGEARTAGDAVIGAAQWAYLDAI
                     HARPDAVEQLRQEYGLAAGDAVPVCYTEISDYAATAERIDHLLALRPAAIGYRLTGAS
                     DLVAEAIGEKAEHLQRFVRELGRRVAMLAPGEDYAPAVYLGLNGALGQLAGDPVRHIG
                     KVLGHVVGLQVATGERQLFLEEPFRLEETLAQAANLHRLKDFIRRTPDSLQRAKPTQV
                     VAPCPLAADAVQVYADTDAVHGLVANSDAGDLHALISGLTRAQTAGLDTYWRLPAGTT
                     PRRAAS"
     gene            2431..2838
                     /locus_tag="PK829_00470"
     CDS             2431..2838
                     /locus_tag="PK829_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39720.1"
                     /translation="MKTTAEQNGRLLTGCALVAVLLLFALAVTLALAIRRDRDLGRYP
                     GATPITSHSNYSGLPRQFRWDNSYFTTDSFNEVDNWYSVTFGLGAESRAMERCILLEG
                     PAEWLWLVRDTSVLLCNAPEGQLIYVTRTTRLR"
     gene            complement(2851..3786)
                     /locus_tag="PK829_00475"
     CDS             complement(2851..3786)
                     /locus_tag="PK829_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOU39721.1"
                     /translation="MTGGLIALLRLARPLFLVGGFVFYGLGVAIARYEGVALHLPALL
                     WGQLAVTAAQLMTHFANDYFDLAADRANSTPTRWSGGSRVLAEGLLPPQVGLWAALVC
                     GGAALAAIGAVWAVRMSAAAAVLLLLATALAWFYSAPPARLHSRGLGEWTVAAIVPVL
                     TPLVGYTVQRGVPGPLPLLAAVPPACFQVAMIMVINFPDAAGDRQVGKRTLVLRLGGE
                     RAARLHPALLALGYLSLPLLVWAGLPPLVAVAVLLPLPLAIWLAWQMSHDAWRDPTAW
                     NALGFWSIGLLMATAGLELLAFLVAGRGMPWLPFG"
     gene            complement(3783..5660)
                     /locus_tag="PK829_00480"
     CDS             complement(3783..5660)
                     /locus_tag="PK829_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39722.1"
                     /translation="MTRQSRSIWWNGRMVVLAVVLVGTLVVLGATGSVQGQGPTRLVP
                     IGAGYEADTLELFAAQAAAHDSDGVVTIRVLPITYHVRPDAILPPIRRENLELAGERA
                     EQIREACQAVAAAGQTCAATVVDIQIRPDALAPERVAQLGPEVDGFYILGGDQTVGMQ
                     VVANTPTEAALAAGFAAGAAVGGNSAGAAVISRYMIGGYAGDNSAWDGLHQGALDLWY
                     GPDSSERRGLIFGLPEAVIDQHVLERGRTARLLQAMVAKPDDKLAVGFDWGTAGVIDE
                     SLLVHDITGWYAAIALDGETYGAAADASYVGDDPAILAIHDVAYHVLPPGPYGYDLTA
                     LLPLVDGVAQPAPDISGRNFDVLLPEEGAGALFVSGDLSAATRGAVMTEFVAAANAAG
                     GPTIVLSAGPRQRAAEGWGRFWRNRLRNMGLAERPTIITLTPATNQTALAARLAGAGA
                     IFFTGNDAVLMADLVAAMRAAGTDTALRDWWLGGGVFLADRAAAAAVGTAMSAEPTPT
                     GGNVEYQSSDSFLADYIHIRPGLGLLPAVVEPRPLYDYLYGRMVSHVMAAPGTVVVGV
                     ERGAALRVTPAEAAMIGDEAALVLDSRYAATLAVGDNDAFAATWLLLDTFPPGTTLAA
                     R"
     gene            5840..6094
                     /locus_tag="PK829_00485"
     CDS             5840..6094
                     /locus_tag="PK829_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39723.1"
                     /translation="MARTDERSRLPTPTTTTAPTRTHRWIAPILGAVLGLILGTAVAS
                     LAAAYMPEGSSAFGGLGLSLQIIGAVVGLFIGGLMARSRR"
     gene            6197..6415
                     /locus_tag="PK829_00490"
     CDS             6197..6415
                     /locus_tag="PK829_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39724.1"
                     /translation="MRPTVSQPLIVLMGSLYGLVGGLVLALVLLSGLVPNGWAEGFVA
                     GPFAFQGLLSAGGAVCGAALTGLRLTKQ"
     gene            6508..7992
                     /locus_tag="PK829_00495"
     CDS             6508..7992
                     /locus_tag="PK829_00495"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39725.1"
                     /translation="MTELDAPALWNLAVDLSHTLVADYARRADLTALGGYDFAPYEQQ
                     LAASLAPGVVAFVACLSAALPSDFDLLPVLTQFGRDPETATALNRYGEDAAAGITAIA
                     AVLTKIGLPAGQMEANALGTALAAWSAASAEVARLNAHLDMTQQALAVTPLYSQQHPP
                     AAEALYADMARLVQALAYQGLSRVAAGDILAADGYTILFSWRRGQSGAQAEPPESAKP
                     ESLPDSAVGEIPGGLEDLLSGTGAEPLPAPPLPVQPAPIPPAPGVPSPVLTLRLDTAA
                     PPQVTVGIPFDLAASVRQLASPALDIADLSLQESASFAAILPSGAAFLAMRIQIAAPE
                     CDILGGDTRPVRVLPGQDGPTVYFQLTPRHAGPLSIIVTVYQEMDWIGSARLRTEAAG
                     GNDGGQTVAAPRGAVALTVNSQPLAPAEVNLKTLRDALDKGYSLSELQDLCFELEIDY
                     EDLPGEGQAAKARELVLFCKRRDLLAVLVEHVMRDRPLLLGDRG"
     gene            complement(8052..9761)
                     /locus_tag="PK829_00500"
     CDS             complement(8052..9761)
                     /locus_tag="PK829_00500"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39726.1"
                     /translation="MWPDVLLLMQLYWRLDRRDVTRVRGWRRVGMIVGLIAFVFIGAF
                     SAAAGYGLSFLTRPESPVQLAPGTVPGILLTLVLFGVLFTGLNQAMRALFLSGDLDQL
                     VVAPIQTRAIMIAKLLSRLPGNIALLLIMAVPAFVAYGIGVGAGVIYYLLGLLLLLLA
                     PLFGLAIGTLISMLLVRWLPVNRLNEMLTATYALLGIFIAILAQLPRFFFVNEETSIQ
                     TLEAAGEMITRVQSLPLPTLLAGRGLMAIDAFQFDGPGLLGVLAYLLLTLGFFLAVVF
                     GGERMYLSGWLKAQSAGSKRRGLEERDNVFGGRSLAVALGIKDWLLRVRDPRQLVTLL
                     GGSFIAIFVSALALFNSNGGQGSLLDFSVSGELQAPGGWAALTAAFQPGVIMSAWSLF
                     AAYVMLSTPAQGALPLERQSFSLLKAAPLRPAEVWRAKTWSVALPAGVVFGLILTVAR
                     FVFPFSLVWMPYALIVGVLMTIALVMLSVSVGFRFANLDWKDPRRMTTSGGGWVGLLL
                     TLVYGVPAIIVAVAPFALAQLWPQWGVLLGLAGITVLALGTWFWARQMARWAERSWEL
                     LPA"
     gene            complement(9751..10521)
                     /locus_tag="PK829_00505"
     CDS             complement(9751..10521)
                     /locus_tag="PK829_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU39727.1"
                     /translation="MTQPVLEANHLSRTFGDFVAVDDVTFSLQPGEIVGFLGPNGAGK
                     TTTIKMLTGLLGPSAGSARVAGHDIVTEPLEAKARIGYVPDTPNLYGKLKASEYLRFV
                     GQLYKVPPARVEERMRPMLDMFDLTDVAGNYLDTFSHGMQQKVAITGAFLHDPQIVFM
                     DEPTVGLDPRSARLIKDLMIRNRDRGRTIFFSTHILEIAQTMCDRVIIINKGRIVADA
                     RVDDLRAMRGEQTLEDIFLELTGGMDVDDMVKELGDVA"
     gene            10857..11987
                     /locus_tag="PK829_00510"
     CDS             10857..11987
                     /locus_tag="PK829_00510"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU39728.1"
                     /translation="MPIATSDLPLPTLETNVGVALEPVQNIQHTLTLLAHAEDVSGYD
                     EWVYRTHASMSAEERDTNLLVMNGLYYATRPRAAYPSFPAYIEHLATVPAIHLRDKVL
                     SAYLRMMPLADDRQLTPEEVLADLNTFMAFLRRGFGDHFILEIERRAYTYLLDPPALQ
                     GLVVGHLRAMWYKYLAVEWERVRPMLQKAVRAFDSIDLSSMDRAAAAEYIVGRPLRKD
                     DKWCDELDDAERLVFVPSAHIGPYTAKIRGEDRTWILFGARQPQDAGEMAPELTRAEL
                     LVRLNALADDSRLNILRLIVDTGEARSADIIQLLDMSQSAASRHLQQLTAAGFLHERR
                     CNGAKCYHLNSDRVDDTLSALASFLARTETRELISPGRAKGE"
     gene            11989..12237
                     /locus_tag="PK829_00515"
     CDS             11989..12237
                     /locus_tag="PK829_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39729.1"
                     /translation="MNANWYDAEQKLNESRAFARAMADAYPLTPARPRHPLRAAYRNT
                     LVLTGGALIALGRRLQGEIQDMAATPLELGQPRQMPTP"
     gene            complement(12421..13059)
                     /locus_tag="PK829_00520"
     CDS             complement(12421..13059)
                     /locus_tag="PK829_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU39730.1"
                     /translation="MTRHPIMAPPTMLATEAQHLMHENDIRHLPVVGDGKRLLGLVDR
                     MSFALDPGALPSLDLWEITRHLSRLSVMDVMTPADSVATIPPDHSVERAARYMTEHGL
                     SCLPVVEEEAVVGLLTKADLLNAFQVLLGLPAEGVRVTMRMPDQPGEFAKLTAVLGRQ
                     SWGVMGMGTFPTPRREGFYDAVVKICGVGVDEVLAVLSQVTGQEVVDIREVV"
     gene            complement(13146..15242)
                     /locus_tag="PK829_00525"
     CDS             complement(13146..15242)
                     /locus_tag="PK829_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU39731.1"
                     /translation="MSTVPVLTVENLAVAYQTRGGDVQAIRDISFEIARAQTFGLVGE
                     SGCGKSTTAWAILNFLGPNGAVKRGRIQFQGQDLLGRTAEELRRLRGDQIAMVYQDPM
                     QALNPSVRIGEQMKEVLTVHRGLSDVAATRRCVAMLERVYMPDPEDILRRYPHQLSGG
                     QQQRVVIATALLNNPALLIMDEPTTALDVTVEAAVLDLIAELRRDFDTAILLITHNLG
                     VIARTCDQVGVMYAGELVERAAVADLFADPQHPYTQGLLRALPRLGADKADSVLYPIR
                     GRVPLPGNRPSGCLFAPRCDYAQELCRAERPALRLLPNGAWVRCHFAEEIDPAAWVPR
                     DDFVPPRPQPAASDSPPILTANGLKKYYSLPGNSLRDILGLGEKRFVHALENATFAVL
                     RGTTLGVVGESGSGKSTLVKTLIGLEASSGGEASFLGFDLTGDLSGRDTALIRELQMV
                     FQNPDATMNPAYTVGYQLARPMRRLGIVPRSQIRAEVVRLLESVRLGENYLDRLPRQL
                     SGGEKQRIGIARALASRPELVLCDEPVSALDVSVQAALLNLLLDVQREYGTTLIFIAH
                     DLSAVRYISDQVAVMYLGEIVDIGPAEAIFAPPYHPYTEALLSAIPSPDPLARQQRIR
                     LAGPVPSAVNPPGGCRFHTRCPRRELLPDGGRICATDIPPWRDADDGHRILCHIPLET
                     LRALPPVGAAPTATSD"
     gene            complement(15259..>15988)
                     /locus_tag="PK829_00530"
     CDS             complement(15259..>15988)
                     /locus_tag="PK829_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU39732.1"
                     /translation="PPNATNWLGTDNLGRDTLSRLMVGTQVVLLKTRVPVGERTIALP
                     LGVAVWGVLVSLALGTLLGQVAGYRRGRWDQGIMQLLDAFVAFPSIVLYLVIIAALGR
                     GDLVVIFAIALTGAPGVARLARSLTLDISTRDYVRAAETRGENLWYILFREILPNARG
                     PLLVDAMLRVGYAVFAIGTLGFLGLGLPPPDPDWGSMVNEARRFMFSSAWGVVWPSLA
                     IASLVVGLSLLADGLREELNRYQR"
BASE COUNT         2966 a         5388 c         5086 g         2538 t
ORIGIN      
        1 ccttagttat actatgtgga gtcacatcac cgttggcgag acaataaacg ctctgccccg
       61 gccaccccgg ccgcaaatgg ataactatcg atgacccgaa ccaataccct cctgcccatc
      121 tccgagcggc cggtgcccaa agggcctatc tggctggacg aggaagcctt cctgcgtgag
      181 gccggcgcgc aaagcctgaa gaccgagacc acctaccgca gcggcctgcg cctcttcgcc
      241 gactggttgc agcatttccg ccgcgctggc tacgccaaaa gcgatgcgtg gccgctcagg
      301 cctgaccatc tgacgacgga cgtcatcctg gaatatcgca actggctgct ggccaatcgt
      361 tcgcgggcca cggtgacgac ctatatggcc gccgtggccg gctatctcaa ctttctcgac
      421 ggctatgacc gtctgcccga caccgtccaa ctgggcaagc tacaacgcca gatggcgcga
      481 cggcagatcg agcgcaacca ggccgaggcg gtcatcgacc tcgacatcgc ccggcaggac
      541 atcccgcgca ttgtggccta ctatgacgga ttgcccctac ccccacagaa cgacgtctac
      601 aatcgccgtc tgtcgctgct gcgcgaccgg gctatcgtca agatgctcta ttcgacggcc
      661 gcgcgcatct ccgaggtcgt ctcgctgaac cgcgccaacg tcggccacgg ccgcgccgcc
      721 tacgccacca tcgtcggcaa gggcaacaag ggccgcacgc tccacattcg cgaatacgcc
      781 cgccaggctg tcgtcgccta cctggccgag cgcaccgacg gcaattcggc gctgttcgtc
      841 tcccacagcc gcaactccac cagcgcgcgg ctgagcatca cgtctgtcca taaggtggtt
      901 aagcgcgccg ttcatgcgtt gcacctgcac gaaagcctgt cggcccacga cttccgtcac
      961 taccgcgcca cccaactcct gcgcgcgggg atgccgttgg aggtcgtgca agagttcctg
     1021 gggcacgccg acgtcaccac gacgcgcaat atctatgccc ctgttctggg cgtgcaggtc
     1081 gtcaccgagt ggttggacaa tctggacgtg ccaccgaagg aagccctgag cgacgtcaac
     1141 gggccgttgg tgacggatga gcggctgttg tcgatgctcg acgacgacta agccccaccc
     1201 tgccctgtgg caacccaccc tgttacacgt agcacgttct gctacacgaa attcacggct
     1261 gccgcagcgc tttggcctcg gccaacaggc tggtcaccag cgccggctcc ccgtcgccct
     1321 ggtcgccgct gacgagcacg gccgacgcac gaccacgctg tgtaatgacc accggctcgt
     1381 nnnnnnnnnn gacgccgccc gccggggcgt ggtcccggcc ggcagccgcc agtaagtatc
     1441 cagcccggcc gtctgcgcgc gcgtcagacc gctgatcaga gcgtgcaggt cgccggcgtc
     1501 gctgttggcg accagaccat gcaccgcgtc cgtgtcggcg tagacctgaa ccgcgtcggc
     1561 ggcaagcggg cagggagcaa ccacttgagt gggtttggct ctctgcaacg agtcgggcgt
     1621 acggcggata aagtccttca gccggtgtaa gttggcagcc tgggcgaggg tttcttcaag
     1681 acggaatggc tcctcaagaa aaagttgccg ctctccggta gccacttgca gcccaacgac
     1741 atggccaagc accttgccaa tgtggcgcac gggatcaccg gccaactgtc ccaacgcgcc
     1801 gttgagtccg aggtagactg ccggcgcata gtcctcgccg ggggccagca tggcaacgcg
     1861 ccgccccaac tcgcgcacga aacgttgcaa gtgctcggcc ttctcgccaa tggcctcggc
     1921 caccaggtcg ctcgcgccgg tgaggcgata gccgatggcc gccggccgca aggccagcag
     1981 gtgatcgatg cgctcggctg tggcagcgta gtcgctgatt tcggtgtagc agaccggcac
     2041 agcgtcgccc gcggccaacc catactcctg gcgcaattgc tcaaccgcat cgggccgggc
     2101 gtggatggcg tcgaggtatg cccactgcgc cgcgccgatc acagcgtcgc cagcggtgcg
     2161 cgcttcgcca atggcggcac gcacgcgcag ggtcacgtcc tcgaggccgc ccaccgattg
     2221 tccggacagg gccattgtga tagggtctct cagcgcgtat acggccgaca ccgccgccgg
     2281 ccgcgccggg ccgtcgggca gaaccacatc gccccaatgc acgacgcctg tgtcgagacg
     2341 caggcctagc gagagcggcc ccgccccacc ctgaggaggg gcggccgggg cgagcaagat
     2401 atctgtgatg atgatcggtc gcaatgccat atgaaaacca ccgctgagca gaacgggcga
     2461 ctcctgaccg ggtgcgcgct ggtggccgtg ctgctgctgt tcgcgctggc agtgacgctg
     2521 gcgctcgcta tccgccgcga ccgcgatctg ggccgctacc ccggggccac gccgatcacc
     2581 agtcacagca actatagcgg cttgccccgc cagtttcgtt gggacaacag ctattttacc
     2641 accgacagct tcaacgaggt cgacaactgg tattcggtca cttttggtct gggcgcggag
     2701 tcgcgggcca tggagcgctg cattcttctg gaagggccgg ccgagtggtt gtggttggtg
     2761 cgcgacacct cggtcttgct ctgcaacgcg ccggaggggc aactcatcta cgtcactcgc
     2821 acgacgcgcc tgcggtagct ccgcgcgcgc tcagccaaag ggtagccacg gcatgcccct
     2881 gccagccaca aggaaggcca gcagttccag accggccgtg gccatgagca gaccgatgct
     2941 ccagaagccc agcgcgttcc aggccgtcgg gtcgcgccat gcgtcatggg acatctgcca
     3001 ggctaaccag attgccagcg gcagcggtag caggacggca acggcaacaa ggggtggtag
     3061 cccggcccaa actaacagcg gcagcgacag gtagcccagc gccagcaggg ccgggtgcag
     3121 ccgcgccgcg cgttcgccgc ccaaccgcag caccagcgtc cgcttgccca cttgccggtc
     3181 gcccgcggcg tcgggaaagt tgatgaccat gatcatcgcc acctggaagc aggccggcgg
     3241 cacggccgcc agcaacggca gagggccagg cacgccacgc tgaacggtat agccgaccag
     3301 cggggtcaac acgggcacga tggcagctac cgtccactcg cccaggccgc gcgagtgaag
     3361 ccgcgccggc ggggcgctgt agaaccaggc cagcgccgtc gccaggagca agaggaccgc
     3421 ggccgcggcg ctcatgcgca cggcccagac tgcgccaatg gccgccagcg ccgcgccgcc
     3481 acacaccagc gcggcccaca gccccacctg tgggggcaac agcccctcgg ccaatacccg
     3541 gctgccgccc gaccagcgcg tcggtgtgga gttggcccgg tcggcggcca ggtcgaagta
     3601 gtcgttggcg aaatgggtca tgagctgggc ggccgtcacg gccaactggc cccagagcag
     3661 cgcggggagg tggagggcca cgccctcata gcgggcaatg gccacgccca gcccatagaa
     3721 gacaaacccg cccaccagaa acagggggcg ggccagtcga agaagagcga taaggccgcc
     3781 agtcatcgcg ccgcgagcgt cgttcctggt gggaacgtgt ccagcagcaa ccatgtggcc
     3841 gcgaaggcgt cattgtcgcc cacggccaga gtggcggcat agcggctgtc gagcacgagg
     3901 gcggcctcgt cgccaatcat cgccgcctcg gccggcgtca cccgcagggc cgcgccgcgc
     3961 tcaacgccaa cgacgaccgt gccgggcgcg gccatgacgt ggctgaccat gcgcccatag
     4021 agataatcat agagcggccg cggctcgacg accgccggca acagccccaa cccgggccgg
     4081 atatgaatgt agtcggccag gaagctatcg ctggactggt actcgacatt gccaccggtc
     4141 ggggttggtt cggcgctcat ggccgtgccc acggcggcgg cggcggcgcg atcggccaga
     4201 aacacgccgc cgcccagcca ccagtcgcgc aatgccgtgt ccgtcccggc cgcgcgcatg
     4261 gccgcaacga ggtcggccat gagcacggcg tcgttgccgg tgaaaaagat cgcgccagcg
     4321 ccggccaggc gcgccgccag cgccgtctgg ttggtcgccg gcgtcagagt gatgatggtg
     4381 gggcgttcag ccagacccat attgcgcagc cggttgcgcc agaagcgccc ccagccctcg
     4441 gcggcgcgtt ggcgcggccc ggccgaaagg acaatggtcg gcccgccggc ggcgttggcc
     4501 gcggccacga attcggtcat caccgcgcca cgggtcgccg cgctcaggtc gccggagaca
     4561 aacagcgcgc cggccccctc ctctggcagt aaaacgtcga agttccggcc gctgatgtcg
     4621 ggcgccggct gggccacgcc gtcgaccagc ggcaaaagcg ccgtcaggtc atagccgtag
     4681 gggccgggcg gcagaacgtg ataggccacg tcgtggatcg ccagaatggc cgggtcgtcg
     4741 cccacgtagc tggcatcggc cgccgcgcca taggtctcgc cgtcgagggc gatggccgcg
     4801 taccaaccgg tgatgtcgtg gaccagcagg ctttcgtcga tgactcccgc cgtgccccag
     4861 tcgaagccca cggcgagctt gtcgtccggc ttggctacca tcgcttgcaa aagcctggcc
     4921 gtccggccgc gctctagcac atgctggtcg atgacggcct ccggcagccc gaatatcagc
     4981 ccgcgccgct cgctgctgtc cggaccgtac cacagatcga gcgcgccctg atgcagacca
     5041 tcccaggcgg agttgtcgcc ggcgtagccg ccgatcatgt agcgagagat gaccgccgcg
     5101 ccggcgctat tgccgccgac ggccgcgccc gcggcaaacc cagccgccag cgcggcctcg
     5161 gtcggcgtgt tggccaccac ctgcatccct accgtctggt cgccgccgag aatgtagaag
     5221 ccatcgacct ccgggccgag ttgagctacg cgctcagggg ccagcgcgtc gggtcgaatc
     5281 tggatatcaa cgacggtggc ggcacatgtc tgcccggcgg cggcaaccgc ctggcaagcc
     5341 tcgcgaatct gttcggcgcg ctcgcctgcc aactccaggt tctcacggcg aatagggggc
     5401 aggatggcgt ccggccgcac gtgataggta atgggcaaca cccgaatcgt cacgacgcca
     5461 tcgctgtcat gggcggccgc ctgcgcagcg aataattcca gcgtgtcggc ctcatagcct
     5521 gcgccgatgg gcaccaggcg cgtcggcccc tgcccctgga cgctgccggt agctccgagc
     5581 acaacgagcg ttcccaccag gacgaccgcc agcacaacca tgcgcccgtt ccaccaaatc
     5641 gaccgcgatt gtcttgtcat cgccatcctc cgacccgtct cgaccgcggc cgacggccct
     5701 ggcccgccga gcctgcggaa tctatcgccg tctatatccg tggattatac cccggtatat
     5761 agccggcgct ccatgcgtct attatcctgt atttacggtg aacgcgagag cagatatggt
     5821 caccaccgga ggaagtgcta tggctcgtac agacgaacgc agtcgattac caacccccac
     5881 cactaccacc gccccgacgc gcacgcatcg ctggatcgcc ccgattctgg gcgcggtcct
     5941 gggcttaata ctgggcacgg ccgttgcctc tctggcggct gcctacatgc ccgaaggctc
     6001 gtcggccttc ggcggtctcg gcctgagttt gcagatcatc ggcgcagtgg tcggtctgtt
     6061 cattggcggg ctaatggccc ggtcacggcg gtaatcagga gcggcacatt ggccgtgccg
     6121 tcacgttggc caggcgcgcc tggccatgag gaggtgcttt gggatgaagc gggaaactac
     6181 cgatagccgg gtcgccatgc gacccacagt ctcgcagcca ctcatcgtgc ttatggggtc
     6241 actctacggg ttggtcggcg gcctggtatt ggccctggtt ctgttaagcg gtcttgtgcc
     6301 gaacggctgg gcggagggct ttgtggccgg ccccttcgcg tttcagggac ttctaagcgc
     6361 gggcggagcc gtctgcggcg cggcgctgac cggtctacgc ctgacgaagc agtaagcgcg
     6421 ggcacaaatc tggcgtagag acgcgcgttt ggtttatact ggcgctcagc acgccggcct
     6481 cctgtcgccg gcggcgcgga gtggcccatg accgagctgg atgccccggc gctgtggaat
     6541 cttgctgttg acctatccca tacactggtt gccgactacg ccagacgcgc cgacctgact
     6601 gctttaggcg gatatgactt cgcgccctat gagcagcagt tggccgcctc acttgcgccg
     6661 ggagttgttg cctttgttgc ctgcctgagc gcggccctgc cctctgactt cgacctatta
     6721 ccagtcctga cgcagttcgg ccgcgacccg gaaacggcga cggcgctcaa tcgctatggc
     6781 gaagatgccg ccgccggtat caccgctatc gccgctgtcc tgaccaaaat aggtctgccc
     6841 gccggccaaa tggaagcgaa cgcgctgggc acagcactgg ccgcctggag cgccgcgtcg
     6901 gccgaggtgg ctcgtctcaa tgcgcatctg gacatgacgc agcaggccct ggcggtcacc
     6961 ccgctttata gccagcagca cccaccggca gccgaggcac tctacgcgga catggccaga
     7021 ctggtgcaag cgctcgctta ccagggcttg agccgtgtcg ctgcgggcga catcctggct
     7081 gccgacggct acacaattct cttctcgtgg cggcgcggcc aaagcggggc acaggcagag
     7141 ccgccggaga gcgccaaacc cgaaagcctg cctgatagcg ccgtcgggga gatccccggt
     7201 ggactggaag acctcctctc cggcacgggc gcggagcctc tacctgcgcc cccactaccc
     7261 gttcagcccg cgcccattcc gcccgcgccg ggcgtgccca gccctgtcct gacgcttcga
     7321 ctcgatacgg ccgcgccgcc acaggtgacc gtcggtatcc ccttcgatct ggccgcgtct
     7381 gtcagacaac tggcgtcgcc cgcgctggac attgccgatc tatcgttgca ggaaagcgcc
     7441 tcgttcgccg cgatcttgcc atctggagcg gcctttctgg ccatgcgcat ccagattgcc
     7501 gcgcccgaat gcgacatcct cggcggcgac acgcggccgg tgcgcgtctt gcctggccag
     7561 gatgggccga cggtctactt ccaactgacg ccgcgccacg ccgggccgtt gagcatcatc
     7621 gtgacggtct atcaggagat ggactggatt ggctcggcgc gcctgcgaac agaagcggcc
     7681 ggcggcaacg atggcggtca gacggtggcc gcgccgcgtg gggccgtggc gttgacagtc
     7741 aacagccagc ctctggcccc ggccgaagtg aacctgaaga cgctccgcga cgcgctggac
     7801 aagggctata gcctgtccga actgcaagac ctgtgctttg agctggagat cgattacgaa
     7861 gacctgcccg gcgaaggcca ggcagccaaa gcgcgcgaac tggtgctctt ttgcaagcgg
     7921 cgcgatctgc tggcggtgct agtcgagcat gtcatgcgcg accggccgct cttgctaggg
     7981 gataggggtt aggttctggg tgcggaaaga tatcgttctt ccctgactct caaaatccag
     8041 tccctatccc cttaggcggg cagcagctcc caggagcgct cggcccagcg ggccatttgc
     8101 cgcgcccaga accaggtacc cagcgccaga accgttatcc cggcgagtcc cagcagcaca
     8161 ccccactggg gccaaagttg tgccagggca aatggcgcga cggcgacgat gatggccggc
     8221 acgccataga ccagggtcag caatagcccc acccacccgc caccgctggt ggtcatccgc
     8281 cgtgggtctt tccagtcgag gttggcaaag cgaaagccga cagacacgct gagcatgacc
     8341 agggctattg tcatcaggac gccgacgatc agcgcatagg gcatccagac gagactgaat
     8401 ggaaagacga agcgagccac ggtcagaata agcccaaaga cgacgcctgc cggaagcgcg
     8461 acgctccacg tcttggcccg ccagacctcg gccgggcgca agggcgcggc ctttagcagc
     8521 gagaaagact gtcgctctag cggcagcgcg ccctgggccg gggtcgagag catcacatag
     8581 gcggcgaaga ggctccacgc cgacatgatg actccaggct ggaatgccgc tgtcagcgcc
     8641 gcccaacccc caggggcttg taactcgccc gatacggaga aatccaacag gctaccctgg
     8701 ccgccattgc tattgaaaag cgccagcgcg ctgacaaaga tggcgataaa gctaccaccc
     8761 agcagggtga ccaactgccg tgggtcgcgg acgcgcaaca gccagtcctt gataccgagg
     8821 gccacggcca gcgaacggcc gccaaagaca ttgtcgcgct cttccagccc gcgccgcttg
     8881 ctgcccgcgc tttgggcctt aagccagccg gataaataca tgcgctcgcc accgaacacc
     8941 accgccagga agaagcccag cgttagcagc agataggcca acacgcccag cagccccggc
     9001 ccatcgaact ggaacgcgtc gatagccatc agcccgcgcc cggccagaag cgtcggcaaa
     9061 ggcaaacttt gcacgcgggt gatcatttcg ccggccgctt ccagcgtctg gattgacgtt
     9121 tcctcgttga cgaaaaagaa gcgcggcaac tgggccaaaa tggcaatgaa gatgcccagc
     9181 agggcgtagg tcgccgtcag catctcgttg agccggttga cgggcagcca gcgcactagc
     9241 agcattgaaa tcagcgtacc aatggccagc ccaaacaacg gcgccagcag caatagcagc
     9301 agccccaaca aataatagat gacgcccgcg ccaacgccga tgccataggc gacaaaggcc
     9361 ggtacggcca tgatcaacag cagggcgatg ttgcctggca agcggctgag cagcttggcg
     9421 atcatgatcg cccgcgtctg aatcggggcg accaccaatt ggtccaagtc gccgctgaga
     9481 aacagggcac gcatggcctg gttcagaccg gtaaaaagca caccgaaaag gaccagtgtc
     9541 agcaatatac cggggacagt gcctggagcg agctgcaccg gcgactccgg ccgggtcaga
     9601 aacgacaggc catagccggc cgcggccgag aacgcgccga tgaacacaaa ggcgatcagg
     9661 ccgacgatca tacccacccg ccgccagccg cgtacacggg tcacgtcgcg ccggtcaagc
     9721 cgccaataaa gttgcatgag caacaggaca tcaggccaca tcgcccaact ccttgaccat
     9781 gtcgtccacg tccatgccgc cggtcagctc caggaagatg tcctccaacg tctgctcgcc
     9841 gcgcatggct cgcaagtcgt ccacgcgggc atcggccacg atgcgcccct tattgatgat
     9901 aatcacccga tcgcacatcg tctgggcgat ctccaaaatg tgggtggaga aaaagatcgt
     9961 ccggccgcgg tcgcggttgc ggatcatcag gtccttaatc agccgggccg agcgcgggtc
    10021 taggccgacc gtcggctcgt ccatgaagac gatctgcgga tcgtggagga acgcgccagt
    10081 gatggccact ttctgctgca tgccgtggct gaaagtatca aggtagttgc cggccacatc
    10141 ggtcagatca aacatatcca gcatcggccg catacgctcc tcaacccgcg caggtggcac
    10201 cttatagagc tggccgacga agcgcagata ctcgctggcc ttcagcttgc catagagatt
    10261 gggcgtatcg ggcacatacc cgatgcgcgc cttagcctcc agcggctcgg tgacgatgtc
    10321 gtggccggct acgcgggccg agccggccga cggccccaga aggcccgtca acatcttgat
    10381 ggtcgtcgtc ttgcccgcgc cgttggggcc gaggaacccg acaatttcgc ctggctggag
    10441 ggaaaaagtc acgtcatcga cggctacgaa gtcgccgaag gtgcgcgata agtggttggc
    10501 ttcaagaacc ggttgggtca tgaatacttc tctcctgtgc gcgccgccgc tgcacactat
    10561 acgcgcaagg gcgccgcagg ttccgcgtgt tggcggggct tgggccgccc cacagtcaac
    10621 atccgtattg tacccgcgaa gtgcccagga accacgcgac cacttgcctg gcccagggtt
    10681 ttgccaaagt cacctcaggc gcgcgggcat tgggcctcat ctaacccatt tatgcggcaa
    10741 atcgcataat acgcaaattg cctcttgaca ggcgctggtt tggcgattat actgcccaca
    10801 ttacccgtaa atgcgggtaa tagggtaaat agctagacac aataaggaag actgcgatgc
    10861 ccattgccac cagcgacctc cccctgccga ctctggaaac gaacgtcggc gttgccctgg
    10921 agccggtgca gaacatccag cacacactga ccctgctcgc gcacgcggag gacgtatccg
    10981 gttacgacga gtgggtctac cgcacccacg ccagcatgtc ggccgaggag cgggacacca
    11041 acttgctagt gatgaacggc ctctactatg ctacccggcc gcgcgccgcc tacccgagct
    11101 tcccggccta catcgagcac ctggccaccg ttccagcgat ccacctgcgc gacaaggttc
    11161 tgtcggccta cttgcggatg atgccgctgg cagacgacag acagctcacg ccggaagaag
    11221 tgttggccga cctcaatacg ttcatggcct ttctgcggcg gggcttcggc gaccatttca
    11281 ttctggagat cgagcgccgc gcttacacct atctgctcga cccgcctgcc ctgcaagggc
    11341 tggtggtcgg ccacctgcgg gccatgtggt acaagtacct ggccgtcgag tgggaacggg
    11401 tgcgaccgat gttgcaaaag gctgtgcgcg cctttgatag catcgacctg agcagcatgg
    11461 atcgggctgc ggcggccgag tacatcgtcg gccggccttt gcgcaaagac gacaagtggt
    11521 gcgatgaact cgacgatgcc gaacgccttg tctttgtgcc ctcggcccac atcggtccct
    11581 acacggccaa aatccggggc gaggatcgga cgtggattct cttcggcgcg cgccagccgc
    11641 aagacgccgg cgagatggct ccggaactga cgcgggccga gctgctggtg cggctcaacg
    11701 cgctggccga cgactcgcgt ctcaatatcc tgcggctgat cgtcgatacc ggcgaggcgc
    11761 gttcggccga catcatacag ctgctcgaca tgagccagtc ggccgcctcg cgccacttgc
    11821 aacaactgac cgccgccggc tttctccatg agcggcgctg caacggcgct aagtgctacc
    11881 acctgaacag cgaccgcgtc gatgacacgc tatcggccct ggcctcgttc ctggcacgaa
    11941 ccgagaccag agaactcatc tcgcccggcc gggcgaaagg agagtgacat gaacgccaat
    12001 tggtatgatg ccgaacagaa gctcaacgag agccgcgcgt tcgcccgggc gatggccgat
    12061 gcctatccgt tgacaccggc ccgtccacgc cacccactgc gcgccgccta ccgcaacacg
    12121 ctggtgctca ccggcggcgc gctcatcgct ctcggccggc gcttgcaggg cgagattcag
    12181 gatatggccg cgacaccgct cgagctgggc cagccgcggc aaatgcctac cccttagcgt
    12241 ttatcccttt tggacgttgc ttggaggaat gccaacaagt cttctcgcca gcatttcgtg
    12301 tcctcctcca tccctccaaa cccgtccaaa acacgacccc ggcagtccag cagcttccgg
    12361 ggtctttttt tgcctcctac cgggccgttg gcggccggca gctctgtggc cggcgcgagc
    12421 ctaaaccact tcgcggatgt caaccacttc ctggccggta acctggctca gaaccgcaag
    12481 gacctcgtct accccgacgc cacagatttt gaccacggcg tcgtagaagc cttcgcggcg
    12541 cggcgttggg aaagtgccca tacccatcac cccccaggac tgccggccga ggacagccgt
    12601 cagcttggcg aactcgcccg gctggtcggg catgcgcatg gtcacgcgca cgccttccgc
    12661 cggcaacccc aacagcacct gaaaggcgtt gagcaaatcc gccttcgtca gcaggccaac
    12721 gacagcctcc tcctccacca ccggcaggca actcagcccg tgctcggtca tgtagcgcgc
    12781 tgcgcgctcc acgctgtggt caggggggat ggtggccacg ctatcggccg gcgtcatgac
    12841 atccatgacc gacaggcgcg acagatggcg ggtgatttcc cacaggtcaa gactgggcag
    12901 cgcgccaggg tctagcgcga agctcatgcg gtctaccagg ccgagcagcc gcttgccgtc
    12961 gccaacgacg ggcagatggc ggatatcgtt ctcgtgcatg agatgttgcg cctcggtggc
    13021 caacatcgtc ggcggggcca tgattgggtg gcgggtcata cagtctcgga cgagcatacg
    13081 cagtcacctc gcaagggcta tccagcgcat ctacgcggcg cggacgcggt gggccttagt
    13141 gggccttaat ctgatgtggc tgtgggcgcg gcccccactg gcgggagtgc gcgcagcgtc
    13201 tccaggggaa tgtggcaaag aatacggtgg ccgtcgtccg cgtctcgcca gggcgggatg
    13261 tccgtggcac agatgcgccc gccgtcgggc aatagctcac ggcgcgggca acgcgtgtgg
    13321 aagcggcaac cgccaggcgg gttgacggcg ctgggtacag gcccggccag gcggatgcgc
    13381 tgctgccgcg ccagggggtc ggggctgggg atggccgata gtagcgcctc cgtgtagggg
    13441 tggtaggggg gcgcgaagat ggcctcggcc gggccgatat cgacgatctc gcccaggtac
    13501 atcacggcca cctgatcgga aatgtagcgc acagcgctga gatcgtgggc gataaaaatg
    13561 agggtcgtgc catactcgcg ctgcacgtcg agcagcagat tcaacagcgc cgcctgcaca
    13621 gagacatcca acgcgctgac cggctcatca cacagcacca gttccggccg gctggccaac
    13681 gcgcgggcga tgccaatgcg ctgtttttcg ccgccgctca actggcgcgg caagcgatcc
    13741 agatagttct cgcccaaccg caccgactcc agcaggcgaa cgacctcagc gcggatctgg
    13801 ctgcggggca caatacccag gcgacgcatc ggccgcgcca gttggtaacc caccgtgtac
    13861 gctgggttca tcgtcgcgtc ggggttctga aagaccattt gcagctcgcg gatgagcgcg
    13921 gtatcgcggc cagagaggtc accggtcagg tcgaagccca gaaacgacgc ttcgccaccg
    13981 ctgctggcct ccaggccaat gagcgtcttc accagcgtac tcttgccgct gccggactcg
    14041 ccgacgacgc ccagcgttgt gccccgaagg acggcaaatg tggcgttctc cagagcgtgg
    14101 acgaagcgct tttcgcccag accgaggata tcgcgcagcg agttgcccgg cagcgagtaa
    14161 tacttcttca ggccgttggc cgtcaggatg ggtgggctgt cgctcgcggc cggctgcggc
    14221 cggggcggta cgaagtcatc gcgggggacc caggcagccg gatcgatttc ctcggcaaag
    14281 tgacagcgca cccacgcccc gttgggcaac aggcgcaacg ctggccgctc tgcgcggcaa
    14341 agctcctggg catagtcgca gcgcggagcg aagagacagc cggatggcct gttgccgggc
    14401 agcggcacgc ggccgcggat ggggtagagc acgctgtcgg ccttgtcggc ccccaggcgc
    14461 ggcagtgccc gcaacaaccc ctgcgtgtag gggtgctgcg ggtcggcgaa gaggtcggcc
    14521 acagcagccc gctcgaccag ttcaccggca tacatcacac ccacctggtc gcacgtgcgg
    14581 gcgatgacgc ccaggttgtg ggtgatgagc aggatggccg tgtcgaagtc gcgtcgcagt
    14641 tcggcgatga ggtcgagcac ggccgcttcg acggttacgt ccagcgccgt cgtcggctcg
    14701 tccatgatga gcagcgccgg gttattgagc agggccgtgg cgatgacaac gcgttgttgc
    14761 tggccgccag agagttggtg cgggtagcgc cgcagaatgt cctctggatc gggcatgtag
    14821 acccgttcca gcatggcgac gcagcgccgc gtggccgcca cgtcgctcag gccgcgatgg
    14881 acggtcaaca cctccttcat ctgctcgcct atgcgcacag aggggttgag cgcctgcatg
    14941 gggtcctggt agaccatggc gatctggtca ccgcgcagcc gtcgcagttc ttcggccgtg
    15001 cgacccagca agtcttgtcc ttgaaactgg atgcgcccac gcttgacagc gccatttggc
    15061 cccagaaagt tgaggatagc ccatgccgtc gtagatttac cgcaacccga ctcccccacc
    15121 aggccaaagg tctgggcacg ggcgatctcg aaagagatgt cgcggatggc ctgcacgtcg
    15181 ccgccacgcg tctggtaagc gacggcgaga ttctcgactg tcaggacggg cacggttgac
    15241 acggtaggtt cctttcgctc agcgctgata gcggttcagt tcctcacgca ggccgtcggc
    15301 caacaggcta aggccgacga cgagcgaagc aatggccagc gacggccaga cgacgcccca
    15361 ggcggacgag aacatgaagc gccgcgcctc gttgaccatc gagccccagt cggggtctgg
    15421 cggcggcagc cccagcccca gaaagcccag ggtaccgatg gcaaacacgg catagccgac
    15481 ccgcaacatc gcgtcgacca gcagggggcc gcgcgcgttg ggcagaatct cccggaacag
    15541 gatgtaccag aggttctcgc cgcgcgtctc cgccgcgcga acgtagtcgc gcgtgctgat
    15601 gtcaagcgtc aggctgcgcg ccaggcgggc cacgcccggc gcgccggtga gggcaatggc
    15661 gaagatgacc accagatcgc cgcggccgag cgcggcgatg atgaccaggt agagaacgat
    15721 gctggggaag gcgacaaagg catcgagtag ctgcatgatg ccctgatccc agcgtccgcg
    15781 ccgatagccg gccacctgcc ccagcagcgt gcccagagcc agcgagacca ggacgcccca
    15841 gacggccacg cccaacggca gggcgatcgt ccgttcaccc acaggtacgc gcgtcttcag
    15901 cagcaccacc tgcgtgccga ccatcagccg ggagagcgta tcccggccga gattgtccgt
    15961 gcccagccag ttggtcgcat tgggcggc
//
LOCUS       DAOSMQ010000007        15702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_3755_length_15702_cov_4.421678, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000007 DAOSMQ010000000
VERSION     DAOSMQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15702
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_15702_cov_4.421678"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1862
                     /locus_tag="PK829_00535"
     CDS             81..1862
                     /locus_tag="PK829_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOU39733.1"
                     /translation="MNRWFRFRRPRLVHLAAILALLAVTALTGGALSTRAQGGAWEGR
                     YWNNRDLSGNPVLIRQDGSIDFDWGGGSPATTVFTDNFSAQWTQTANLPAGTYRFSAT
                     ADDGMRVWVDNVLIIDAWYDSQVRTITADVFLGAGNHTIVVQYYEAGGVAVAKMNYVS
                     LNAPPPPTPGNNWFNEYFTNTSLSGTPALTGTTTDINFNWGLGTPTAGFPADFFSVRW
                     VRNLTLDAGRYRFTTTTDDGVRLWVNNVLVIDQWRVQPPTSFSVDIDLPGGAIPIKME
                     YFENTERAYAKLTWQRIGTTPPPTSSAWRAEYFSNMSLSGQPVVVRDESAVDYNWGYG
                     TPAPQVPVDHFSARWTANLNFPSGRYRFSATSDDGVRVWVNNQLIIDAWFDRAAQTST
                     AEFTSPGGVLPVRIEYYENTALAEMRFGYTSLTGTGGQFPGTGTVTSYRLNVRTGPGT
                     NFAVITRLSTGNVVNLTGYRNGDATWVQVSLANGTVGWASALYLRTTIPVANLTPITG
                     TTTPPTPPAGAQGTVNTGALNVRTGPDVGYPAFTSISNGTTVTLLARNAASTWVKVIL
                     RDGRQGWVNASFLILNVSINSLPVSNL"
     gene            2002..2553
                     /locus_tag="PK829_00540"
     CDS             2002..2553
                     /locus_tag="PK829_00540"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOU39734.1"
                     /translation="MLSFDIPPLTTDALLGALFIFALRVFGITISTIRVLVMMRGKKA
                     AAFIFGFIEVLAYVVAIAEVVNNLDNIWNVLGYCLGFSVGTVVGMIYDERTASGYANV
                     RIISRYQAQRIIEQIHEAGYGATVGYGHGRDGTVGMIVAVVRRQDAEAVCVIAETADP
                     NAFITIEDARAVRRGHLYMGQER"
     gene            2623..2862
                     /locus_tag="PK829_00545"
     CDS             2623..2862
                     /locus_tag="PK829_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39735.1"
                     /translation="MPAITLLDGSLKLTIYYDAADREFEDNICLQFEEDSVEFESLFH
                     AEETSLYLTPEQAALIVLELSRALEEARRDKPGPP"
     gene            2924..3775
                     /gene="panB"
                     /locus_tag="PK829_00550"
     CDS             2924..3775
                     /gene="panB"
                     /locus_tag="PK829_00550"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOU39736.1"
                     /translation="MERRKMTAPRLVAKKARHEPITMLTAYDYTGARLVDAAGIDAIL
                     VGDSLGMVMMGLDNTLPVTMEEMLHHCRAVARGAKYAHLVGDMPFMSYQAEVAEAVRN
                     AGRFLREGGMESVKLEGGQEMVATVRAIVAAGIPVMGHLGLTPQSISKFGGMRAQGKT
                     AVAARRLVDDALALEAAGCYAIVLEAVPAPVAAAVSQRLAIPTIGIGAGAGCDGQVLV
                     YHDVLGLFDRVHPPFVKEYARLSETIVDAFGAYREDVVAGRFPAEEHTYPMSAEELAA
                     FQQSLEG"
     gene            3777..4760
                     /locus_tag="PK829_00555"
     CDS             3777..4760
                     /locus_tag="PK829_00555"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOU39737.1"
                     /translation="MRVAILGIGALGCLLGAHLEPVADVVLLGHWPAQLAALRRDGLR
                     LEHPDGSHSHHRLAVADDPAAAGRANVVLVAVKSRQTPDAARAAERLLGPDGLALTLQ
                     NGLNNRAALRAVLGEARVALGVTAQGATTLAPGVVRHAGHGPTYLGRDAALGPAQRDC
                     LSEIATLFGRAGFETALVDDADGLAWGKLVANAAINPLTALLRVPNGFLLEHAALVAI
                     MRQTAAEVAAVAERQGIALPEGDAGERAIAVARGTAANRSSMLQDVERGTLTEIDAIC
                     GAVVRAGRAAGVATPLNSRLASLVAQVESGNPPVAPGDVAGLIRQLDPAMR"
     gene            4772..5635
                     /gene="panC"
                     /locus_tag="PK829_00560"
     CDS             4772..5635
                     /gene="panC"
                     /locus_tag="PK829_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOU39738.1"
                     /translation="MISDIASVRALRRGYGDATLGFVPTMGYLHEGHMELVRRARREN
                     DHVAVSIFVNPTQFGPTEDLSRYPRDLERDLALLRAEGVDFVFAPGDGVIYPPDFQSS
                     VLVRGVTQPLEGARRPTHFEGVATVVAKLFNIVRPTRVYFGQKDAQQVVVVRQMVRDL
                     NFDLDVVVCPTVREPDGLAMSSRNKYLEGDQRRAATVLNRALRAAEHLWNEGKRDGEA
                     LREAMRDTLAAEPLARVDYVSAADPQTLAEYEGTISPDAGVLLSMAVFIGRTRLIDNF
                     NLAGTAEQTGE"
     gene            5628..6377
                     /locus_tag="PK829_00565"
     CDS             5628..6377
                     /locus_tag="PK829_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOU39739.1"
                     /translation="MSSPATLPPALLDSLRRAGRVVVLTGAGISAESGVPTFRQAQTG
                     LWAQYDPQELATPAAFRRQPQLVWDWYAWRRELVAGVAPNPGHFALASMEAYVPTFTL
                     ITQNVDSLHQRAGSRHVIELHGNITRVKCSREERVVETWPATEERPPRCPDCGAFLRP
                     DVVWFGEALPAAVLHAASEAARLADIFLTIGTSGLVQPAASLPRLAAGNGAVTVEINI
                     EDTPLSPRMDYTLRGPSGVILPMLVERAWPD"
     gene            6451..6702
                     /locus_tag="PK829_00570"
     CDS             6451..6702
                     /locus_tag="PK829_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39740.1"
                     /translation="MSDQAMPLHPSLLEFLRCPLAVRSAEHGDDPGRLRLVHGSWLVC
                     DESGMKYPIRDGIAIMLIEEGEKWRNTAEADLPVPPPAA"
     gene            6711..7073
                     /locus_tag="PK829_00575"
     CDS             6711..7073
                     /locus_tag="PK829_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU39741.1"
                     /translation="MKTILQIEDNYANTRLVERVLEHQGYRLLHAADGRTGIDIALQE
                     HPDLILLDMGLPDMDGQTLVAVLRGAAGLVHTPIVALTAWPEATAAQMAVNYGCDGCI
                     TKPINVTEFAEQIAAYLR"
     gene            7111..8043
                     /locus_tag="PK829_00580"
     CDS             7111..8043
                     /locus_tag="PK829_00580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU39742.1"
                     /translation="MILLPASEFTLDELTLAYNQSRTDYLIPMPMNPDRLNEYITLYD
                     VDLHRSRVAVTGESIVGLGMLGRRQDQGWVTRLGVLPEGRRQGIGRALLGELLHQAAN
                     SGMPEVWLEVIKGNQPALELFRSYDFQPTRELIIARRPPRAVANAAALLAARKIYYLQ
                     HGEVIDLHCARHERMNWLNAVDSMRNVRRLAAASVGSDPHDGVPLHQLPHLSGLLVEF
                     HDGSRGWVTYLATTLELKRISVEVTYGPPDVVAACLLELLHRHHAAQDAVVENIPDDV
                     RWRGFQRAGYFEVFRRIEMVRPAENVTYTNEFSP"
     gene            8057..8980
                     /locus_tag="PK829_00585"
                     /pseudo
     CDS             8057..8980
                     /locus_tag="PK829_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
     gene            9110..10777
                     /locus_tag="PK829_00590"
     CDS             9110..10777
                     /locus_tag="PK829_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU39743.1"
                     /translation="MATLAAASQVDFLPDAGEETLTARIGDYHALIVGPDQHISNHIL
                     KYGYNLRAIGTLGGRLNNIDVSAARALGIEVCYAPDSRAVAIAEHTVARLLSLAENLG
                     DGRLAGKTLGLIGFGLVGQLVAQRAAAFDMRILTNQPRLTPELALAPGVESTDLIALL
                     RQSDFISLHVPFSEETEAIIGARELAAVKPSAMLVNMGHTDLVDDEALLRALDAGGLA
                     GVALSALPALVTNPAPASLAVRRHPRVRVSPHVSAVLDDQRRDAALQMARQITAALQV
                     RRANETLNLEIVPIELVTPHEHIDQKRVTRLMERLEVDGRLVNPPVTTYWKGRYVILD
                     GATRYSALQRLGYRFAIVQVVDETQAGFQLHTWYHAISAESESPADFALLEARLRSIA
                     GLHLRPLAPESARQALEQPQALCYFLTRDGNLLLAELAGGASRLAVMNALVDAYNAWG
                     NVERTLLTDTERLLAQFPRLVAVAIFPQFAPAEVFDAAAEGDLLPAGLTRFVIPGRIL
                     RLNADLERLKRDEPPAEKRAWFNEFLGGKLSRSRLRYYQEPVVLLDE"
     gene            10770..11384
                     /locus_tag="PK829_00595"
     CDS             10770..11384
                     /locus_tag="PK829_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU39744.1"
                     /translation="MSSVIRAVIFDIGGVFVRTFDPTGRQQWEARLGLPAGGAEAIVL
                     NSDMGHRAQRGEIEEEALWAWVGEQLALGAALALFRHDFWRGDGVDARLVDLVQRLRP
                     GYQTAVISNATGNLRAVLEHYRLLALFDVVVGSAEEGLMKPHPAIYERALGLLGRTAG
                     ETIFIDDAPANVAGALAVGMNAIRFTPDLDLEAELRRYGVVPAP"
     gene            complement(11400..12995)
                     /locus_tag="PK829_00600"
     CDS             complement(11400..12995)
                     /locus_tag="PK829_00600"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOU39745.1"
                     /translation="MPAVLQRPLEAATRDLTIVHLELFQTVQTATNSVSLIADKPTLL
                     RVYARATGSGVAPVAEVTVHALRGMASLGTLTIGPHVVSSQPLLGDLNTTFNIDLPAE
                     WLAGDVTLMATIDAVNTVPEMNETNNGYTAQFTFHTVAPLQITIVPITYTDTRTGRVF
                     ADPPHDPISDWLRGAFPVSHVTTAFHTAFAFTGDLRQPSEWERLLDNLTTLWAAEVGM
                     DAPTVYYGLIPNADAGGATWFEGGVSGLGWIGQRVSLGLDVGVATGNAAGHEIGHNFG
                     RQHAPCGNPNSVDPHYPYPNATIGVYGVNTDNDVLLDPSANYDMMSYCGPEWVSDYTY
                     EALFHDQVARTSRAAQRSSDGLLLRATLVGDEAIILPVYRLSGPLTSGDNSGYQVQLL
                     DAAGEVLATHPVALLQAEEAGVQTQMLVAHVPAPSGVTALRFVRQGRVLAERALIGPG
                     PEQAASVSAEVRATTEGLALAWSRPDVPALVRYSADGQQWTTLAVDALSGRLNLAGIQ
                     LPAGGQIQVVPGDGSPVITLALD"
     gene            complement(13391..15643)
                     /gene="topA"
                     /locus_tag="PK829_00605"
     CDS             complement(13391..15643)
                     /gene="topA"
                     /locus_tag="PK829_00605"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020621258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU39746.1"
                     /translation="MAGKSAKTSAAKTRKDAKSSAGGGERGGKLVVVESPAKAKTISR
                     YLGPGYVVKPSVGHVRDLLKSRLSVDVENDFAPEYRVPNEKRALVKELKEAAARADEI
                     FLATDPDREGEAIAWHVREAAEMDPARTKRVVFQEITPPAIRAAFAAPRAIDMDRVEA
                     QQARRVLDRLVGYQLSPLLWRKVQGRLSAGRVQSVAVRLVVEREREIEAFDSREYWTI
                     GAELSQQKFRSLPANNGNRRPFFLGRLHRLDGAEPELSSEAAVAPHLAALEQAHWEVG
                     EVTLGKRTRRPAAPFTTSTLQQEASRKLGLGTTRTMRLAQELYEGVNVGEGNVGLITY
                     MRTDSVTVAAVAQEQARAFIAETYGADYLPDKAPVYKTRSKTAQEAHEAIRPTAVTRT
                     PAALKEHLSREQLRLYTLIWQRFVASQMAPALYDTVSVDIWAGPARTPAAQRPYLFRA
                     SGSTLAFRGFLAVYGADDPDEDNGTEGDGPQVPTDLRAAEALDLLQLLPEQHFTQPPP
                     RYSEASLVKALEEHGIGRPSTYASIISTIQARGYIDQEQKRLRPTEIGSIVNDMLVQY
                     FPDLLSVDFTARLEDELDEIAEGKPWVPVIGQFYQKFEKDLGVADAALPKLDLRKEPE
                     LVGRDCPLSGHPLVYREGRFGRFIGCSDYPRCKYTEQILVRVGVRCPKCGGELVEKRT
                     RKGRVFYGCANYPECDWTSWKQPLAMPCPVCQGVMVQENKELAKCTRCEHVMPLADVA
                     MPASPESIAE"
BASE COUNT         2635 a         5429 c         4935 g         2703 t
ORIGIN      
        1 attgacagcg cgtccttgtg cgctgcgtcg tggcagcttg ggcggctccg cacatgttgc
       61 cagtcaacag gagcatcctc atgaacagat ggttccgctt tcggcgcccg cgacttgtcc
      121 atctggccgc aattctcgcc ctgctggccg ttaccgccct gaccggtggc gcgctcagca
      181 cccgtgccca gggcggtgcc tgggaaggcc gctactggaa caaccgcgac ctgtccggca
      241 atcccgtcct cattcgccag gatggcagca tcgatttcga ttggggcggc ggctcgccgg
      301 ccacaacggt cttcaccgac aacttctccg cccaatggac ccagaccgcc aacctgcccg
      361 cgggcaccta ccgcttctcg gccacggccg atgatggcat gcgtgtttgg gtcgataacg
      421 tgctgatcat cgacgcctgg tacgatagcc aggtgcgcac gatcaccgcc gatgtcttcc
      481 tgggcgcggg caaccacacc atcgtcgtcc agtactatga ggccggcggc gtggcggtgg
      541 ccaagatgaa ctacgtctcg ctcaacgcgc cgcccccacc gacgccgggc aacaactggt
      601 tcaacgagta cttcaccaat accagcctga gcggcacgcc ggcgttgacg ggcacgacca
      661 ctgacattaa cttcaactgg ggtttgggca cgcccacagc cggcttcccg gccgacttct
      721 tctccgtacg ctgggtgcgc aacctgaccc tcgacgccgg ccgctaccgc ttcacgacaa
      781 cgaccgacga cggcgtgcgc ctgtgggtca acaatgtcct ggtcattgat cagtggcgcg
      841 tccagccgcc aacgtctttc tcggttgaca tcgatctgcc cggcggcgct atccccatca
      901 agatggagta cttcgagaac accgagcggg cctacgccaa gctcacctgg cagcgcatcg
      961 gcacgacgcc gccgcctact tcctcggcct ggcgggcgga atacttcagc aatatgagcc
     1021 tgagcggcca gcccgtcgtc gtccgcgacg agagcgcggt cgactacaac tggggctatg
     1081 gcacgcccgc gccccaggtt ccggtcgatc acttctccgc ccgctggacg gccaacctga
     1141 acttcccgtc cggccgctat cgcttctcgg ccaccagcga cgacggcgtg cgcgtctggg
     1201 tcaacaacca gctgatcatc gacgcctggt tcgaccgggc cgcgcagacc tccacggccg
     1261 agttcacctc ccccggcggc gtgctgccgg tgcgcattga gtactacgag aataccgctc
     1321 tggccgagat gcgcttcggc tacacgtcgc tgaccggcac aggcggccaa ttccccggca
     1381 cgggcacggt cacctcctat cgcctgaatg tccgcaccgg gccgggcacg aacttcgccg
     1441 tcatcacccg gttgagcacg ggcaacgtcg tcaacctgac cggctaccgc aacggcgacg
     1501 cgacctgggt acaggtctct ctggccaatg gcaccgtcgg ctgggccagc gccctctact
     1561 tgcgcacgac catccccgtg gccaacctca cgcccatcac cggcacgacc acgccgccca
     1621 cgccgcccgc cggcgctcag ggcacggtca acaccggcgc gctgaacgtc cgcaccggcc
     1681 ctgacgttgg ctaccccgct ttcaccagca tcagcaacgg cacgaccgtg acgctgctgg
     1741 cgcggaatgc cgcctcaacg tgggtcaaag tcatcctgcg cgacggccgc cagggctggg
     1801 tcaacgccag cttcctcatc ctgaacgtgt ccatcaatag tctgccggtt agcaacctgt
     1861 agaccaatag gtggctgcca caagtccatc agggcgctcc cgcggcggcg tgggagcgcc
     1921 cttttcgttg gcggccgccg cgccgcgggt acaataggcc gccatagaga cccagcaggg
     1981 gctgcaactg aaaccaacgc catgttgtcc ttcgatatcc caccgctgac caccgacgcc
     2041 ttgctgggcg cgctattcat tttcgccctg cgcgtcttcg gcatcaccat cagcaccatc
     2101 cgcgtcctgg tgatgatgcg cggcaagaag gcggcggcct tcatcttcgg cttcatcgaa
     2161 gtgctggcct acgtcgtcgc catcgccgag gtcgtcaaca atctggacaa catctggaac
     2221 gtgctcggct actgcctggg cttctcggtc ggcacagtgg tcggcatgat ctacgacgag
     2281 cgcaccgcca gcggctacgc caacgtgcgc atcatctcgc gctatcaggc ccagcgcatc
     2341 atcgaacaaa tccacgaggc cggctacggg gccaccgtcg gctatggcca cggccgtgac
     2401 ggcaccgtgg gtatgatcgt cgccgttgtc cgccgccagg acgccgaagc cgtctgcgtc
     2461 atcgccgaga cagccgaccc caacgccttt atcaccattg aagacgctcg cgccgtccgc
     2521 cgcggccacc tctacatggg tcaggagcga taacagcagt cttattgatt gcggcgacgc
     2581 ccacgactga tgcgtagcgt cgccgctgga ggtacagaat ctatgcctgc catcactcta
     2641 ctagatggga gcctcaaact gacaatttat tacgatgccg ccgacagaga attcgaggac
     2701 aacatctgtc ttcagtttga ggaagacagc gtggagttcg agagcctctt ccacgctgaa
     2761 gagaccagcc tctacctcac gccagagcag gccgccctca tcgtgttaga actgagccgg
     2821 gcgctagaag aagcgcggcg cgacaagccc ggccccccgt agcccacccg cgccgccctc
     2881 cgaccaggag cacgctgccc ggccacaaac aggagcaatg acaatggaac gcagaaaaat
     2941 gaccgccccg cgactcgtgg ccaaaaaggc acgccacgaa ccgatcacca tgctgaccgc
     3001 ctacgactac accggcgcgc gtctcgtcga cgccgcgggc atcgacgcca tcctcgtcgg
     3061 tgactccctg ggcatggtca tgatgggcct ggacaatacg ctgcccgtca ccatggagga
     3121 gatgctccac cactgccgcg ccgtcgcccg tggggccaaa tacgcccatc tggtgggcga
     3181 tatgcccttt atgtcctacc aggccgaagt ggccgaggcg gtgcgcaatg ccggccgctt
     3241 tctgcgcgag ggcggcatgg agagcgtcaa gttggaaggt ggccaagaga tggttgccac
     3301 cgtccgcgcc atcgtcgccg cgggcatccc ggtcatgggt cacctgggtc tgacgccgca
     3361 atcgatcagc aagttcggcg gcatgcgcgc tcagggcaaa accgccgtcg ccgcccgccg
     3421 cctcgtggac gacgccctgg ccctggaagc ggccggctgc tacgccatcg tcctggaggc
     3481 ggtgcccgcc ccggtcgctg ccgccgtcag ccagcgtctc gccatcccga ccatcggcat
     3541 cggcgcgggc gccggttgcg atggccaagt gctggtttac cacgacgtgc tgggactctt
     3601 cgaccgcgtt catccgccct tcgtcaagga gtatgcccgg ctgagcgaga ccatcgtcga
     3661 cgcctttggc gcctaccgcg aggatgtcgt cgccggccgg ttcccggccg aggagcacac
     3721 ctaccccatg agcgccgaag aactggccgc gttccagcag agcctggaag gctaagatgc
     3781 gcgtcgccat cctcggcatc ggcgcgctgg gctgcttgct cggtgcgcac ctggaaccgg
     3841 tggccgatgt cgtcttgctc ggccactggc cagcgcaatt ggccgcccta cgacgcgacg
     3901 gcctgcggct ggagcacccc gacggcagtc atagccacca ccgcctggcc gtggccgacg
     3961 accccgccgc cgccggtcgc gccaacgtcg tcctcgtagc cgtcaagagc cgccaaacgc
     4021 ccgacgccgc gcgggcggcc gaacgtctgc ttggccccga cggcctggcc ctgacgttac
     4081 aaaacggcct gaacaaccgc gccgccttgc gcgccgtcct cggcgaggcg cgcgtcgccc
     4141 tgggcgtgac ggcccagggg gcgacgacgc tggcccctgg cgtcgtgcgc cacgccggcc
     4201 acgggccgac ctacctgggc cgcgacgcag ccctcgggcc ggcgcaacgc gactgcctga
     4261 gcgagatagc caccttgttc ggacgcgctg gattcgagac cgctctagtt gacgacgccg
     4321 atgggcttgc ctggggcaag ttggtggcca atgcggccat caacccgctg acggccctac
     4381 tgcgcgtgcc caatggcttc ttgctggagc acgccgcgct ggtggctatc atgcgccaga
     4441 cggcggccga ggtcgccgcc gtggccgaac gccagggcat tgccctgcca gagggcgacg
     4501 cgggcgagcg cgccatcgcc gtcgcccgcg gcacggccgc caaccgttcg tcaatgctcc
     4561 aggacgtaga gcgcggcacg ttgaccgaaa tcgacgctat ctgtggcgcg gtcgtccgcg
     4621 ccgggcgggc cgcgggtgtg gccacgcctc tgaacagccg cctcgccagc cttgtggccc
     4681 aggtcgagag tggcaacccg ccggtcgcgc cgggcgacgt ggccgggctg attcgccaac
     4741 tcgacccggc gatgaggtaa agaccgtgga agtgatcagc gacatcgctt ctgtgcgggc
     4801 gctccgccgc ggctacggcg acgctacgct tggctttgtg cccacaatgg gctatttaca
     4861 cgaagggcat atggaactgg tgcggcgggc gcgacgcgag aacgaccacg tcgccgttag
     4921 catcttcgtc aatccaaccc aattcggccc caccgaggac ctcagccgct acccgcgcga
     4981 cctggagcgt gacctggccc tcttgcgtgc cgagggcgtc gatttcgtct tcgcgcccgg
     5041 cgacggcgtc atctatccgc ctgatttcca gtcgagcgtc ctcgtccgcg gcgtcaccca
     5101 gccactggag ggcgcgcgcc ggccaaccca cttcgaaggc gtggccactg tcgtggccaa
     5161 gctgttcaac atcgtccggc cgacgcgtgt ctactttggc cagaaagacg cccagcaggt
     5221 tgtcgtcgtc cgccagatgg tccgcgacct caactttgac ctcgacgtcg tcgtttgccc
     5281 caccgtgcgc gagccggacg ggctggccat gagttcgcgc aacaagtacc tggagggcga
     5341 ccagcgccgc gcggcaacgg tgctcaaccg cgccctgcgc gccgccgaac acctctggaa
     5401 tgagggcaag cgcgatggcg aagctctgcg tgaggccatg cgcgacaccc tggcggccga
     5461 accgctggcc cgggtagact acgtcagcgc cgccgacccg caaacactgg ctgaatacga
     5521 gggcacgatc tcgccggacg cgggcgtgct gctgtcgatg gccgtcttca tcggccgcac
     5581 gcgcctgatc gataacttca acctggctgg cacggcggag cagaccggtg agtagccccg
     5641 cgaccctccc gccggcgctg ctcgacagcc tccggcgcgc cggccgcgtc gtcgtgctaa
     5701 ccggcgcggg catctccgct gagagcggcg tgcccacctt ccgtcaggcg cagacaggcc
     5761 tgtgggcgca atacgacccg caggagctgg ccacgccggc ggccttccga cgacagccgc
     5821 aactggtgtg ggactggtac gcctggcggc gcgagcttgt cgccggcgtg gctcccaacc
     5881 ccggtcactt tgccctggcc agcatggagg cctatgtccc gactttcacg ctcatcactc
     5941 agaacgtcga cagcctccat cagcgtgccg gcagccgcca cgtcatcgag ctacatggca
     6001 acatcacccg cgtgaagtgc tcgcgtgaag agcgcgtcgt cgaaacctgg ccggcgaccg
     6061 aggaacgccc gccgcgctgc ccggattgtg gcgcgttcct gcggcccgat gtcgtctggt
     6121 tcggcgaggc gcttccggcg gccgtcctac acgcggccag cgaggccgcg cggctggccg
     6181 atatcttcct gaccattggc acgtccggcc tggtgcagcc agcagcgtcg ttgccgcggc
     6241 tggccgccgg caacggcgcg gtaacggtgg agatcaacat cgaggatacg cccctctccc
     6301 cgcgaatgga ttacacgttg cgcgggccat cgggcgtcat cttgcccatg ctggtcgaga
     6361 gagcgtggcc ggactgaccg cgcccgccag ttggctctcg ccgccccgtt ctgctatgat
     6421 tggccaaccc gtcaatcgct ggtgagaatg atgtccgatc aggcaatgcc cctccaccca
     6481 tccttgctgg aattcctgcg ctgtcccctg gccgtccgct cggctgagca cggcgacgac
     6541 cccggccggc tgcgccttgt ccacggcagt tggctggtgt gcgatgagtc gggtatgaag
     6601 tatcccatcc gcgacggcat cgccatcatg ctcattgaag agggcgaaaa gtggcgcaac
     6661 acggccgaag ccgacctgcc cgtgccgccg ccggctgctt gaaggggcct atgaagacca
     6721 ttttgcagat tgaggacaac tacgccaaca cacgcctggt agagcgcgtg ttagaacacc
     6781 agggctatcg cctcctccat gccgccgacg gtcgcaccgg catcgacatc gccctccagg
     6841 agcaccccga cctgatcctg ctggacatgg gcctgcccga tatggatgga cagacactgg
     6901 tggccgtgtt gcggggcgcg gccgggctag ttcatacgcc catcgtcgct ctgacagcct
     6961 ggccggaagc gactgccgcc cagatggccg tcaattatgg ctgcgatggc tgtattacta
     7021 agcccatcaa tgtcactgaa ttcgccgagc agattgccgc gtacttacgt taggccctat
     7081 gcctgacacg cttcacagaa gtctcgtgaa atgatcctat taccggcgtc tgaatttacg
     7141 ctcgatgaac taaccttagc ctataaccag tcacgcaccg attatctcat tcccatgccg
     7201 atgaaccccg atcggctgaa tgagtacatc accctctacg acgtcgacct gcaccgctcg
     7261 cgggtcgctg tcacggggga gtctattgtt ggactgggca tgctgggcag gcgacaggac
     7321 cagggttggg tcacacggct gggcgttctg ccggaggggc ggcgacaggg catcggccgt
     7381 gccctcctgg gcgagctatt acatcaggcc gccaatagtg gcatgcccga ggtgtggttg
     7441 gaagtcatta agggcaacca gccggcgctg gagctgttcc ggagctacga tttccagccc
     7501 acgcgcgaac tgatcatcgc ccgccgcccg ccgcgcgccg tcgccaacgc cgccgctctc
     7561 ctggcagcgc gaaaaatcta ctatctgcaa cacggtgagg tcattgatct gcactgcgcc
     7621 cggcacgagc gcatgaactg gctcaacgcc gtcgacagca tgcgcaatgt gcggcggctg
     7681 gcggccgcct ctgtcggcag cgaccctcac gacggcgtgc ccctccatca gctaccgcac
     7741 ctgtccggcc tgttggtcga gttccacgac ggctcgcgcg gctgggtcac ctacctggca
     7801 acgacgctag aactcaagcg catcagcgtc gaggtgacct atggcccacc ggatgtcgtc
     7861 gccgcctgcc tgctggaatt gttacatcga caccatgccg cccaggacgc cgttgtcgag
     7921 aacattcccg acgacgtgcg ttggcgtggg ttccaacggg caggctactt tgaggtgttc
     7981 cggcgcatcg aaatggtgcg cccggccgaa aatgtgactt acaccaatga gttcagtccc
     8041 tgagacaagg acgcgtatga tctccccagc agcagtccaa gccgccgccg agcgcctcgc
     8101 cggcgtcgcc catcgcactc ccgtgctgac ctcgcaaacg ctagacaccc gattgggcgt
     8161 gagcgtcttc ttcaagtgcg agaacttcca gcgcatgggc gcattcaagt tccgtggggc
     8221 ctacttcgcc atcagccgtc tgtctgagac tgagcgcgcc gccggggtca tcacccactc
     8281 cagcggcaac catgcccagg ggctggcgct ggcggcgagg ctgctgggcg tgcgcgccgt
     8341 ggtcgtcatg cccgccgacg ccccgccgaa caagcgcgcc gccaccgagg gctacggcgc
     8401 tgaggtcatc ccctgcgacg cgctctatcg cgaggaagtc accgccgaac tgatcgccca
     8461 acacggctac acgctcgtcc atcccttcga caacgacaat atcatcgccg ggcagggcac
     8521 ggccgcgctg gaactcttcg acgaagttgg gccactggac tatcttttcg ttcccgtcgg
     8581 cggcggcggc ctgatcagcg gcagcgccct ggccgcgcaa ttgcgcgcgc cggagtgccg
     8641 cgtcgtcggc gtggagccgg cgctaggagc cgacgccaac cgctcctggc gcagtggcga
     8701 ggtctacaag ctggaggccg ttccggcgac catcgccgac gggctgcgga cgcgggccat
     8761 cggcggccgt aacctggcca ttatgcgccg ctatgtcagc gacatgacca cggccaccga
     8821 agaggagatc ctggcggcgc tggagttcat ctgggcgcgg ctgaagatcg tcgtcgagcc
     8881 gagttcggca gtcgctctgg ccccgctact ggccggcagc tacaccgtgc cgtccggcgc
     8941 gcgcgtcggt gtgttgctca gcggcggcaa cgtcaacgtg tcggctttct tgcagccacc
     9001 ggcggccgcc tccgcccagg ccacagccgc cgcgcgcgaa atggacgctc cggccaggcc
     9061 ccgcacggct tcgcgcattc tcacctgtgt gccgctggaa ggcccggcga tggcaacact
     9121 ggccgctgcc agccaggtcg acttcttgcc cgatgctggc gaagaaacgc ttaccgcccg
     9181 catcggcgac taccacgccc tcatcgtcgg ccccgaccaa cacatcagca atcacatcct
     9241 caaatacggc tacaatctgc gcgccattgg caccctgggc ggtcgcctga acaacatcga
     9301 cgtcagcgcg gcgcgcgccc tgggcatcga agtctgctat gccccggaca gccgcgccgt
     9361 ggctatcgcc gagcacaccg tcgctcgcct gctgtctctg gccgagaacc tgggcgacgg
     9421 ccgtctggcc ggcaagacgc tgggcctcat cggctttggc ctcgtgggcc aactggtggc
     9481 ccagcgcgcc gccgccttcg acatgcgcat cctgaccaac cagccccgcc tgacgccgga
     9541 actggccctg gctcccggcg ttgagtccac tgacctcatc gccctgctgc gccaatctga
     9601 ctttatctcc ctgcacgtgc ctttcagcga ggaaacagag gctattatcg gcgcgcgcga
     9661 actggccgcg gttaagccct cggccatgct ggtcaacatg ggccacacgg acctggtcga
     9721 cgacgaggcc ctgctgcgcg ccctcgacgc tgggggcctt gccggcgtgg cgctgtcggc
     9781 gctgccggca ttggtgacca accccgcgcc ggcatccctc gctgtgcgcc gccatccgcg
     9841 cgtgcgcgtc tcgccccatg tttccgccgt cctggacgac caacgccgcg acgccgcgct
     9901 gcaaatggcc cgccagatca ccgccgcgct gcaagtccga cgtgcgaacg agacgctcaa
     9961 tttggaaatc gtccctattg agctggtcac gccccacgag catatcgatc agaagcgcgt
    10021 aacgcgcctc atggagcggc tagaggtaga cggccgcctc gtcaatccgc cggtgacgac
    10081 ctactggaaa gggcgctacg tcatcctcga cggggccacg cgctacagcg ccctgcaacg
    10141 actcggctac cgttttgcca tcgtccaggt cgtcgatgag acgcaggcgg gtttccagct
    10201 gcacacctgg tatcacgcga tttccgctga gtcagagagt ccggccgact ttgcgctgtt
    10261 ggaagcgcgc ctgcgctcca ttgccggcct ccacctgcgc ccgctggctc cggagtccgc
    10321 gcgccaggcg cttgaacagc cccaggcgct gtgttacttc ctgacacgcg acggcaacct
    10381 gctgctggcc gaactggccg gcggcgcgtc ccggctggcg gtcatgaatg ccctcgtcga
    10441 tgcctacaac gcctggggca acgtcgagcg caccttgctg accgatacag agcgcctgct
    10501 ggcccaattc ccgcgcctgg tggctgtggc catcttcccc cagttcgcgc cagccgaggt
    10561 ctttgacgcc gccgccgagg gcgacctgct gccagccggc ctaacccgct tcgtcatccc
    10621 tggccgcatc ctgcgactta acgccgacct ggagcgcctg aagcgcgacg agccgccggc
    10681 cgaaaagcgc gcctggttca acgagttcct gggcgggaag ctctcacgca gccggctgcg
    10741 ctactaccag gagccggtgg tgcttctcga tgagtagcgt catccgcgcc gtcatcttcg
    10801 acattggtgg cgtctttgtg cgcacgttcg acccgaccgg gcggcaacag tgggaggcgc
    10861 gtctgggtct gcccgcgggc ggggccgagg ctatcgttct gaacagcgac atgggccatc
    10921 gcgcccagcg cggcgagatt gaggaagagg ccctgtgggc ctgggtgggt gagcaattgg
    10981 cgttgggcgc ggccctggcc ctctttcggc acgacttctg gcgcggcgac ggcgtcgatg
    11041 cgcgcctggt cgatctcgtt cagcggctgc ggcccggcta ccagacggcc gtcatcagca
    11101 atgcgaccgg caacctgcgc gccgtgctgg agcactatcg gctgctggcc ctgtttgatg
    11161 tcgttgtggg gtcggctgag gaagggctga tgaaaccgca tccagccatc tacgagcgcg
    11221 ccctggggct gctgggccgt acagccggcg aaacgatctt catcgacgac gcgccggcca
    11281 atgtggccgg cgcgctcgcc gtgggcatga atgccattcg cttcacgccc gatcttgatc
    11341 tggaagcgga actgaggcgc tacggcgttg tgcccgcgcc ctaagcccgc tggcccgcgc
    11401 tagtccaacg ccagggttat aaccgggctg ccgtcgccgg ggacgacctg aatctggccg
    11461 ccggccggca gttggatgcc tgccagattg agccgcccac tcagcgcgtc aaccgccaac
    11521 gtcgtccatt gctggccgtc ggccgagtaa cgcacgagcg caggcacgtc gggccggctc
    11581 caggcgaggg ctaggccctc cgttgtcgcc ctcacctcgg cgctgacaga cgcggcctgt
    11641 tctggcccgg ggcctatcag cgcgcgctca gcaagcacgc ggccctggcg cacgaaacga
    11701 agcgcggtga cgccggacgg cgcgggcacg tgcgcgacga gcatctgggt ttggacgccg
    11761 gcctcctcgg cttgcaagag ggcaaccggg tgggtggcca gcacctcgcc agcggcgtcg
    11821 agcagttgaa cctggtagcc gctgttgtcg ccggacgtga gcgggccgct cagacggtaa
    11881 acgggcagaa tgatcgcctc gtcgcctacc aacgttgccc gcagcagcaa tccgtcgcta
    11941 gagcgttggg ccgcccggct ggtacgcgca acctggtcgt ggaaaagggc ttcataggtg
    12001 tagtcggaaa cccactccgg gccacaatag ctcatcatgt cgtagttggc gctggggtca
    12061 agcagcacgt cattgtctgt gttcacgcca tacacgccaa tggtggcatt cgggtaggga
    12121 taatgtggat cgacgctgtt agggttgccg cagggggcgt gctggcgacc gaagttgtga
    12181 ccgatctcgt gtcctgcggc gttgcccgtg gccacgccca catccagacc cagagacacg
    12241 cgctgcccga tccagcccag gccagaaacg ccaccctcga accaggtcgc gccgccagcg
    12301 tctgcgttgg ggattagccc atagtagacc gtcggcgcgt ccataccaac ctcggcggcc
    12361 cacaaagtcg tcagattatc cagaagccgt tcccattcgc tcggctggcg caaatcgccg
    12421 gtgaacgcaa aggcggtgtg aaaggctgtg gttacgtggc taactgggaa tgcgccccgg
    12481 agccagtcgc tgataggatc gtggggcggg tcggcgaaga cccggccggt gcgtgtgtcc
    12541 gtataggtga tcgggacgat agtgatctgc aagggagcga cggtgtggaa ggtgaactgc
    12601 gccgtgtagc cgttgttcgt ctcgttcatc tccggcacgg tgttgacggc gtcaattgta
    12661 gccatcaggg taacatcccc cgcgagccac tccgccggca agtcgatatt gaaggtagtg
    12721 ttcaggtcgc ccagcaacgg ctgggacgaa acgacgtgcg ggccgatggt taaagtgccg
    12781 agcgaagcca tgccgcggag cgcatgaaca gttacctcgg ccaccggagc tacgccggag
    12841 cctgtcgccc gggcatagac ccgcagcagg gtcggtttgt cagcaatcaa gctaactgag
    12901 ttagtcgctg tttgtacggt ctggaaaagc tccaggtgga caatcgtcag gtcgcgcgtg
    12961 gccgcctcca gtgggcgttg caggacggcc ggcatataga ccttctgggc ctgacggcgt
    13021 tgcgaagctg catgagtggt ccccacactc cccaaatgcg ttagggcagc ggccagcagc
    13081 aacacaacga tggcgatgag cctcgttggg cgctcttgca ttaggtttct ccgtctgctc
    13141 tcgcgcggcg gggtgggtct aactcgcaac aacaagcccc cgaagggcta tcatttggca
    13201 acacacagtg cagtccccgc cgcacttttt caagtgtagg cgcactgcgt taaaaactgc
    13261 gttacaacaa atgaccgtct gttgcggcgt tttggtacag ccggtctgta gcccttcttg
    13321 gccccacaga gcagggccgg ggctacgaat tgactacgaa cgtgtggaag atgatcaggg
    13381 ccggcgcagg ctattcggcg atgctctcag gcgacgccgg catcgccaca tcggccagcg
    13441 gcatcacgtg ttcgcagcgt gtgcacttgg ccaactcctt gttttcctgc accatgaccc
    13501 cctggcaaac agggcaaggc atggccagcg gctgcttcca gctcgtccaa tcgcactccg
    13561 ggtagttggc gcagccatag aagacgcgcc ccttgcgggt gcgcttttcg accagttcac
    13621 cgccacactt gggacagcga acgccaaccc tgaccaggat ttgctcggta tatttgcagc
    13681 gcgggtagtc gctacaaccg ataaaccggc cgaagcgtcc ctcgcgataa accagcggat
    13741 ggccgctcag cgggcagtcg cgcccgacaa gctccggctc cttgcgcagg tctaacttcg
    13801 gcaaggccgc gtcggccaca cccaggtcct tctcaaactt ctggtagaac tggccgatga
    13861 ccggcaccca cggcttgccc tcggcgatct cgtccaactc gtcttccaac cgggcggtga
    13921 agtcaaccga gagcagatcg gggaagtact gcaccagcat gtcattgacg atgctgccta
    13981 tttcggttgg ccgcaaccgc ttttgttcct gatcgatgta gccgcgcgcc tgaatggtgg
    14041 agatgatcga cgcatacgta gagggacggc caatgccgtg ctcttccagc gccttcacga
    14101 gcgacgcctc gctgtagcgc ggcggcggct gggtgaagtg ctgctctggc aacagttgga
    14161 gcaagtccag cgcctcggcc gcgcgcagat ccgtcggcac ctgcggccca tcgccctctg
    14221 taccattgtc ctcgtcgggg tcatcggccc catagacggc caggaagcca cggaaagcca
    14281 acgtcgagcc gctggcccga aacaagtaag ggcgctgggc cgcgggcgtc cgcgccggcc
    14341 cggcccaaat atccaccgag acggtgtcgt atagagcagg cgccatctgg ctggcgacga
    14401 accgttgcca gatgagcgtg tagagccgca actgctcgcg ggagaggtgc tctttcagcg
    14461 ccgccggcgt gcgcgtcacc gccgtcggcc ggatggcctc atgcgcctcc tgggctgttt
    14521 tggagcgcgt cttgtagacg ggggccttgt cgggcaggta atcggcgcca tatgtctcgg
    14581 caatgaaggc gcgcgcctgc tcctgggcca cggcggccac agtgacgcta tcggtgcgca
    14641 tgtaggtgat caggccgacg ttgccctcgc cgacattgac gccctcatat agttcctggg
    14701 ccagacgcat cgtgcgcgtc gtgcccagtc ccagcttgcg cgacgcttcc tgttgcagcg
    14761 tactggtggt gaatggggcc gccgggcggc gcgtgcgctt gcccagcgtc acttcgccca
    14821 cttcccagtg cgcctgttcc aacgccgcca ggtgcggcgc tacggcggcc tcactggaca
    14881 gttccggttc ggccccgtcg aggcgatgca ggcggccgag gaaaaacggc cggcggttgc
    14941 cgttgttggc cggcagcgaa cggaacttct gctggctgag ttctgcgccg atggtccagt
    15001 actctcgcga atcgaacgcc tcgatctcgc gctcgcgctc gaccaccagg cgcacggcca
    15061 cactctgcac gcgcccggcc gacaagcgcc cctggacttt gcgccacagc aacgggctaa
    15121 gctggtagcc gaccagccgg tcgagcacgc gccgcgcctg ctgcgcctcg acgcgatcca
    15181 tgtcaatggc ccgcggcgcg gcgaaggcgg cacggatggc tggcggggtg atctcctgga
    15241 agacgacgcg tttcgtccgc gccgggtcca tctcggccgc ctcgcggacg tgccaggcga
    15301 tggcctcccc ttcgcggtcg gggtcagtgg ccaggaaaat ttcgtcagcg cgggcggcgg
    15361 cctccttaag ttctttcacc agggcgcgct tctcgttggg cacgcgatac tccggcgcga
    15421 aatcattctc cacatcgacg ctcaggcgtg acttcagcag gtcgcgcacg tggcccacgc
    15481 tgggcttcac cacgtagccc ggccccaggt agcggctgat ggtcttggcc ttggccggtg
    15541 attcgacaac gaccagctta ccgccgcgct cgccgccacc ggcgctgctt tttgcgtctt
    15601 tgcgggtctt ggcggctgag gtcttggcgc tcttgccggc caccgaagcc ttttggcctt
    15661 tgcccgcgac cgttgtccgg ccactcttgc cggccgctgg gg
//
LOCUS       DAOSMQ010000008        15459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_3840_length_15459_cov_4.812516, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000008 DAOSMQ010000000
VERSION     DAOSMQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15459
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3840_length_15459_cov_4.812516"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PK829_00610"
     CDS             complement(<1..191)
                     /locus_tag="PK829_00610"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU39747.1"
                     /translation="MDESDALLVVERGPAPSLRFALKADQVTIGRSAGNELVMADPEV
                     SRRHARVLRRADGYAVEDIG"
     gene            complement(236..943)
                     /locus_tag="PK829_00615"
     CDS             complement(236..943)
                     /locus_tag="PK829_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39748.1"
                     /translation="MNALRRLTYAYLSRMYALMYFLSRRPFLSALVRWLPLAALFIGW
                     LQGWPPAALLVVALVAVWINYSLWRARRDNYNRFVADARPLGDAPPEPPLPPNQRLEV
                     LATGLFSVSGRESRLFLRPARYWHVPLGDHIIMAEEEPGKYLYQFFSATTLQELREGL
                     LLYGTQPTPALAVSFLARWGPEHTRFGQVYELGQDEGPPPKRITVYLTSADENTRRLI
                     RETIISTARRARLSGVA"
     gene            complement(988..1716)
                     /locus_tag="PK829_00620"
     CDS             complement(988..1716)
                     /locus_tag="PK829_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU39749.1"
                     /translation="MLSLDRQNYWREVYAAEHPGWRPATERFAAHVRAALRPGARLLD
                     LGCGRGGLVEQLDDPAGVVGIDPDFGSLRDHRLPGFPRAAALSDRLPFAAASFDVVAA
                     SWLLEHLAAPAVTFGEIARVLRPGGAFVFITPNGRHPLAWANRAGGRLGRAQGRLVAR
                     LYGRSGADTFPTVYRANAPDVLARLAAAAGLAVETFDCVADPTYLAFSPALFRVMVAV
                     EDRLPPGRRIHFVGVLRRPAGASG"
     gene            1816..3873
                     /locus_tag="PK829_00625"
     CDS             1816..3873
                     /locus_tag="PK829_00625"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOU39750.1"
                     /translation="MRAFILILLLALSTAACSTRQTPAIPPTPVAVTVAPTATPPPTA
                     LSLTRPPEPPPAADATPAPTAIAPAVPTLAPTAPPDEARATAAAELAGAPPPADPVRL
                     AIAYRGLNPALPTAAPPDRPQLGDVETFTIRNVDNITDSQITARLLSISDTAYFWFDQ
                     AADVPQPDAAELDAVAAQFDDIYAALYAEFGVAEPPGGKAHIVHVSPASLCVNADNCG
                     LAGYFNSVDLLPRAVNPQSNERAMFVMNARQYGSGRYLDVLAHELRHMLGATYDTGDE
                     DWFIEGAATLAQDLTGFSNGPQERGSLFLQNPDQQLNSWSDDNTLPHYGQGYLVNRFL
                     YDRLGVALYDEYATSPAVGLGAVDGVAAAHGLDVTGEGLWRDWLVAMAVHDNADAPER
                     YHWGGPALEPVATTRVDNTPATFDTTVSQYAADYYELPSSGSFLIDFAGAPAVSLLGA
                     GAPSGDYFWVAQRANDSNPRLTRAIDLRGVDAQRGDEAMLTYRVFADIERGYDFAYVS
                     VSTDGGERWQALTAGHMQGLSPDDDPSGSALTERFYTGRTRAWVDEAIDLTPFAGQEI
                     LLRFEVVTDLILSYTGLALDDIAIPAIGFADDAETLDAGWLAEGFVRATATLPQTWPL
                     QLVTFDRDEQPTVQALPVGADGRVQFVLEATPGARRPLLIVAATAPDTLQPASYTLRV
                     GEP"
     gene            complement(3900..3998)
                     /locus_tag="PK829_00630"
     tRNA            complement(3900..3998)
                     /locus_tag="PK829_00630"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3962..3964),aa:Sec,seq:tca)
     gene            4124..4196
                     /locus_tag="PK829_00635"
     tRNA            4124..4196
                     /locus_tag="PK829_00635"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4157..4159,aa:Phe,seq:gaa)
     gene            4226..4297
                     /locus_tag="PK829_00640"
     tRNA            4226..4297
                     /locus_tag="PK829_00640"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4258..4260,aa:Gly,seq:gcc)
     gene            4338..4408
                     /locus_tag="PK829_00645"
     tRNA            4338..4408
                     /locus_tag="PK829_00645"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4370..4372,aa:Cys,seq:gca)
     gene            4561..6285
                     /locus_tag="PK829_00650"
     CDS             4561..6285
                     /locus_tag="PK829_00650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOU39751.1"
                     /translation="MSLTPIPNLPFAIDTTSWEGFQARYEALTNEPLNEATLADWLAR
                     WSQLNMLVEESGAIANIEKTLDTADETAERAFLNFVENIAPNFSRADQALKQRLLAAA
                     TDNDALGEDMRVPLRAMRNQADLFREENVPLMTELAKLGNEYDKITGALSADWDGEPR
                     NLNQLNALLRDRDRAVRERAWRQMMALWLGQRAALNDLYGRMLALRRQMAANAGLPNY
                     RDYIYRAYNRFDYTPDDCLRFHDAIAAVVVPAAQRVYERRRARLGLDALRPWDLAVDP
                     SDAPPLAPYHGQDELIQGSLNIFERVDPGLARQFALMAEENLLDLDTRAGKALGGYCS
                     SLVWRRRPYIFMNGVGSHDDVQTTLHEAGHAFHAFESFALPTIWQMEVPLEFCEVASM
                     GMELLAAPYLTRASGGFYTPAEAARARSEHLEGILTFLPYMAVVDSFQHWVYTHPDAA
                     ADPAACDAAWDGLWARFMPGVDWSGFEAERATGRHRKLHIFHIPFYYIEYGLAQVGAL
                     QVWRNALHNQAQAVATYRDALKLGGTRSLPELYRAAGAEFRFDEPMLRDLVDLIETTL
                     AELEATEP"
     gene            6287..6955
                     /locus_tag="PK829_00655"
     CDS             6287..6955
                     /locus_tag="PK829_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOU39752.1"
                     /translation="MDARESLAPSAAMREYLAEIYRLQEDSPTVSTTSLADRLNVSPP
                     AIPRMLKRLQSVGFVKHVPYQGVELTERGREEALREIRRHRLLEVFLVNVMGFTWDET
                     HEHADDLSAGLNDRLTERMAEMTGQPTRCPHGEPIPDRDGRLPEVNDICIVNLSVGHR
                     GAVSRVRTHEPEKLRYFASLGLTPGTAIEIVGRAPFNGPMRLRAGREEVVIGYELMRS
                     LWVT"
     gene            complement(6973..7404)
                     /locus_tag="PK829_00660"
     CDS             complement(6973..7404)
                     /locus_tag="PK829_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39753.1"
                     /translation="MAKKLFASKVFRSRPLLNVPQVIFLLVIAGAIIIAIDLNRRAQA
                     GRLVGSGEEALRVQVEGEATRQVQLQATLAYVTSDDYVAAYARNESGMILPGERRVVP
                     LLQEATPEPTALPPPTPDPALEARPWQAWWRLLTDAPQPTR"
     gene            7631..8497
                     /locus_tag="PK829_00665"
     CDS             7631..8497
                     /locus_tag="PK829_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU39754.1"
                     /translation="MSVPALAQPPVDFLAVGHICHDLVPGGRLTGGAAAYSSLTAVAL
                     GLRAGVVTSAAAEDDWAAVFPQLAVTQVPAPATTVFENVYTPAGRVQTLHAVAGRLRP
                     EHVPAGWTRAPIVLLGPIANEIDPAIIHLFSDSIVGVGPQGWLRRWDAAGRVWPTAWE
                     GAAEVLPLAAVTFLSEEDLLDAAMLDDTRRLARIVVLTQGERGCTVFCRDEARAFPAP
                     PVDVVDPTGAGDIFAAAYLVRFYQTDGNAWAAAEFANDIAAHAITRRGLPAKSQIIRQ
                     TLDALMHNPAAR"
     gene            8503..9297
                     /locus_tag="PK829_00670"
     CDS             8503..9297
                     /locus_tag="PK829_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOU39755.1"
                     /translation="MAHIYAIANQKGGVGKTTTVVNLTAYLAGSGRRVLLVDVDPQAN
                     ATSALGFDKYQMQPSTYELLVEQKPAAVVRRRQAEYKIDLLPSSPSLAGAEIELVQAI
                     GREYRLQRALQAVADEYDYILIDSPPSLGLLTVNALTAARDGVLIPVQCEYLPLEGLT
                     QLTQTIQLVQEYLNPTLGIRGVMMTMYDSRTNLSRQVVEEVRQHFPNKVFKTIIPRNV
                     RLSEAPSFGQPINFYAPQSPGGLAYKLLATELLNGDRRNGGGEHGR"
     gene            9287..10141
                     /locus_tag="PK829_00675"
     CDS             9287..10141
                     /locus_tag="PK829_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU39756.1"
                     /translation="MAAKRQVLGRGLDALFATGDRTTRADAVRQVAIDLIAPNPRQPR
                     TTLDAEKLSELAASIKAHGLIQPLIVTETAEGYVLIAGERRWRASRLAGLADVPVIVK
                     ETTPQDMLELALIENIQRADLNALEEAHAYRQLTEEFGLTHEAVAERVGKARTTVTNL
                     LRLLALPEPIQAAVSDERLSGAHARALLPLPTPEAQLAAMNQILKLNLSVRQTEALVS
                     SLMADRRPAPRPRKQLPPELAALQGRFEQTLGTRVSIEQGSQGGRIVIHYYSDEELNA
                     IYEAIAGE"
     gene            10254..>11193
                     /locus_tag="PK829_00680"
     CDS             10254..>11193
                     /locus_tag="PK829_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU39757.1"
                     /translation="MLAVQRISKSFGLSPILKDISFALGPGERLGLIGPNGGGKTTLL
                     RILTGQETPDAGHVARTPADLRVGYLPQGLTIDPRATLGDVLHAAAPDAGRLAAELGE
                     LAVRLAAEPGRADVQHAYDDVLQRLSRSEGGRAEGVLAHLGLDSLPREQPVGRLSGGQ
                     KTRLALALVLLDEPDVLLLDEPTNHLDIDMLEWLEAWLANFSGAALIVSHDRTFLDRT
                     VTRILDLDPTTQTVREYAGNYSDYLDQYLSARERQYTTWRHQEDEIRRMRADITRTRE
                     QARHVEITTRPNQPGVRRIAKKVARKAASREKKLERY"
     assembly_gap    11194..11293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11294..12046
                     /locus_tag="PK829_00685"
     CDS             <11294..12046
                     /locus_tag="PK829_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU39758.1"
                     /translation="AKKVARKAASREKKLERYVAADERVERPERSWQMKLAFDTLSEA
                     HIGRDVLTLEDLTVGYDRPLLAGLNLSIRAGQRVVLTGPNGAGKTTLLRTIAGRVPPL
                     AGRARLGASVQLGYMSQEQEHLDPDATPLATILGVAAMTETEARSFLHYFLFEGDDAL
                     RPIAALSFGERSRLSLARLVAAGSTFLLLDEPINHLDIPSRARFEEALTGFPGTILAV
                     VHDRYFIERFATDVWLVEGERVTWLPQEVEGF"
     gene            complement(12119..12304)
                     /locus_tag="PK829_00690"
     CDS             complement(12119..12304)
                     /locus_tag="PK829_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39759.1"
                     /translation="MRSLRSTFFIGFFIALLVAAIVWYYQKSTTTEDGALDLLDRYAE
                     SQRRVEELEQAMAEARG"
     gene            complement(12316..12714)
                     /locus_tag="PK829_00695"
     CDS             complement(12316..12714)
                     /locus_tag="PK829_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39760.1"
                     /translation="MKKAATNPFTRFLSQWTADDDFAAFVAGWDRLERVVVGVYRQKM
                     DATEARAEYIAVWPGLRQSYPRWEAALRPHWQATRAAGERTHSDPFALLLALDGPAAI
                     PGDWRAMQHLPAAREAINRYLRGQSAPDNA"
     gene            13091..14116
                     /gene="hisG"
                     /locus_tag="PK829_00700"
     CDS             13091..14116
                     /gene="hisG"
                     /locus_tag="PK829_00700"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOU39761.1"
                     /translation="MERTDIRLALPSKGILQDGAIEFLEACGLRVFRPNPRQYAATIP
                     ALPGVTVMFQRPGDIVTSIQQGSVEFGISGYDVLAERGANGDSAVLVIHEALGFGPCA
                     LKLAAPEDAPPRDVAELPGWAAELAAAGRPLRVATKFPRLTAAFLDSHGIAPYQLVAV
                     EGTLEIAPAIGYADLICDLVSSGITLRDNHLRPLAGGVVLRSEAVLIANRAALRRRPE
                     ALATARHLLEYIEAHLRAQGSYLLTANVRGESAEAIGRRMFAQTTLGGLQGPTVAPVV
                     AREHLRAEGGWYAVNIVVRKERLFEAVGELRAIGGSGVVVVPCAYIFEEEPERYRAMV
                     EQVGAGW"
     gene            14198..>15459
                     /gene="hisD"
                     /locus_tag="PK829_00705"
     CDS             14198..>15459
                     /gene="hisD"
                     /locus_tag="PK829_00705"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOU39762.1"
                     /translation="MITIYTTDEAQQTILRRERALAPELTAPLRARMLALFGEALTPA
                     AAVARILADVRARGDAALFDWTQRLDGVALTRLDVPAEDIRAAAAALPADLRQSLQLA
                     ADRIRAFHALQPVHSWTTGALGGRLGQRVAPLRRVGVYVPGGTAPLPSSLLMSAIPAQ
                     VAGVAEIVVVTPPGGDGRVPPVILAAAATAGVETLYTLGGAQAIAALAFGTASIGRVD
                     KIVGPGNLFVTLAKQQVFGQVGIDGLAGPTETMIIADETANPAWLAADLLAQAEHDAL
                     AAAILLTPSRALAEAVQREVARQLEALSRDAVIAAALAGQGGIVLTADLDEAVALANA
                     YAPEHLCVATAAPRALADRLHNAGGLFLGERSFEVLGDYVAGPSHTMPTGGTARFASP
                     LNVLDFVRITSLIELDAATTAALAPAAAR"
BASE COUNT         2484 a         5444 c         5117 g         2314 t
ORIGIN      
        1 ccgatgtctt cgacggcata gccgtcggcg cggcgcagca cgcgggcgtg ccgccgtgag
       61 acttccggat cggccatcac caactcgttg ccggcgctgc ggccaatagt cacctggtcg
      121 gctttcaggg caaagcgcag gctgggcgcc ggcccacgtt cgacgacgag aagcgcatcg
      181 gattcatcca tggtctctaa ctatagcctc cttgggcggg cgcggcgaat cgcggctagg
      241 ccacgcccga cagcctggcc cgccgcgcgg tgctgatgat cgtctcgcga atgagccggc
      301 gcgtgttctc gtcggccgac gtgagataga cggtgatccg tttcggcggc ggcccttcgt
      361 cctggcccag ttcgtagacc tggccgaagc gggtatgctc cggcccccag cgggccagga
      421 agctgacggc cagcgccggc gtgggctggg tgccatagag cagcagaccc tcgcgcaact
      481 cttgcaacgt ggtcgcggag aagaactggt agagatactt gcccggctct tcttcggcca
      541 tgatgatgtg gtcgcccagg ggaacgtgcc aatacctggc cggacgcaga aacagccggc
      601 tctcccggcc gctgacgctg aagaggcccg tggccagcac ctccagccgt tggttgggcg
      661 gcaagggcgg ctcagggggc gcgtcgccaa gcggtcgcgc gtcggcgacg aagcggttgt
      721 agttgtcgcg cctggcccgc cagagggagt agttgatcca cacggcgacg agggccacaa
      781 ccagcagcgc cgccggcggc cagccctgca gccagccgat gaatagggcc gccagcggca
      841 gccagcgcac cagcgcgctc agaaacggcc gccggctgag aaagtacatg agcgcgtaca
      901 tgcggctgag ataggcgtag gtgagccgtc ggagcgcgtt catggtctgt attctaggcg
      961 attgtcacgc aaatgtcacc gtatggatca cccactggcc cccgccggcc gccgcaggac
     1021 gccaacgaag tggatgcgcc gcccgggcgg caggcgatcc tccacggcga ccatgacccg
     1081 gaagagggcc ggcgagaagg ccagataggt ggggtcggcc acgcagtcga acgtctccac
     1141 agccagcccg gccgcggcgg ccagccgcgc cagcacgtcc ggcgcgttgg cccggtagac
     1201 ggtggggaag gtgtcggctc cgctccgccc gtagagccgg gccaccagcc gcccctgcgc
     1261 ccggcccagc cgcccgccgg cgcggttggc ccaggcgagc gggtggcgac cgttgggcgt
     1321 gatgaagaca aacgcgccgc ccggccgcag cacgcgagcg atctcgccga aggtcactgc
     1381 cggcgcggcc agatgctcca gcagccagct ggcggcaacg acatcgaacg acgccgcggc
     1441 aaagggcagg cggtcactga gcgccgcggc gcgggggaaa cccggcagac ggtggtcgcg
     1501 tagcgagccg aagtcggggt cgataccgac gacgccggcg gggtcgtcga gctgctcaac
     1561 cagcccgccg cggccgcagc ccagatccag gagtcgcgcc cccggccgca gcgccgcgcg
     1621 aacgtgcgcg gcgaagcgct cggtcgccgg tcgccagccg ggatgttcgg cggcgtagac
     1681 ctcgcgccag tagttctgcc gatccagtga gagcatccag ccattgtacc gcgccggctg
     1741 agaatcggct gatagcgtgg ccggcgagag cggattgagt ttctctggcc cagtccgcgg
     1801 agtagaatcc ccatcatgcg cgcatttatt ctgatcctgc tgctggccct ttcgaccgcg
     1861 gcctgctcga cccgccagac gccggccatc ccaccgacgc ctgtggccgt caccgtggcc
     1921 ccgacggcga cgccgccgcc cacggcttta tcgctgacgc ggccgccgga gccgccgccc
     1981 gccgctgacg ctacgcccgc gccgacggcg atagccccgg ccgtgcccac cctcgcgcct
     2041 accgctccgc ccgacgaagc gcgtgccacc gcggcggccg aactagctgg cgcgccgccg
     2101 ccggccgacc ccgtgcgact ggccatcgcc taccgcggcc tgaacccggc cctgcccacg
     2161 gccgcgccgc cagacagacc gcagctcggc gacgtggaga ccttcaccat tcgcaacgtc
     2221 gataacatca ccgacagcca gatcaccgcc cggctgctga gcatcagcga cacggcctac
     2281 ttctggttcg accaggccgc ggacgtgccc cagccagacg cggccgagtt ggacgcggtc
     2341 gccgcccagt ttgacgacat ctacgcggcg ctctacgccg agttcggcgt ggccgagccg
     2401 ccgggcggca aggcccatat cgtccacgtc tccccggcct cgctgtgcgt caatgccgac
     2461 aattgcggcc tggctggcta cttcaacagc gtcgatctcc tgccgcgggc ggtcaatccg
     2521 cagtcgaacg agcgcgccat gttcgtcatg aatgcccggc aatatggctc cggccgctat
     2581 ctcgacgtgc tggcccacga attgcgccac atgctcggcg caacctatga cacgggcgac
     2641 gaagattggt tcatcgaggg cgcggcgaca ctggcgcaag acctgaccgg cttcagcaac
     2701 ggcccccagg agcgcggcag ccttttcttg caaaaccccg accagcaact caatagctgg
     2761 agcgatgaca ataccctgcc gcactatggc cagggctatt tggtcaatcg attcctctat
     2821 gaccgcctgg gcgtggccct ctacgacgaa tatgccacca gcccggccgt cgggctgggc
     2881 gcggtcgatg gcgtggccgc ggcgcatggg ctggacgtga ccggtgaggg gctgtggcgc
     2941 gactggttgg tggcgatggc cgtccacgac aatgccgacg ccccggagcg ctatcactgg
     3001 ggcgggccgg cgctggagcc agtggccacg acgcgggtag acaacacccc ggccactttc
     3061 gacaccaccg tgtcgcaata cgccgccgac tactacgagc tgccgtccag cggctctttt
     3121 ctgatcgact ttgccggcgc gccggccgtg tcgctgctgg gggcgggcgc gccctccggc
     3181 gattacttct gggtggccca gcgggccaac gacagcaatc cacgcctgac gcgggccatc
     3241 gacctgcgcg gcgtggacgc acaacgcggc gacgaagcca tgctcaccta ccgcgttttc
     3301 gccgacatcg agcgcggcta tgatttcgcc tacgtgtctg tttccacgga cggcggcgaa
     3361 cgctggcagg cgctgacagc cgggcacatg caggggctta gccccgacga cgacccttcc
     3421 ggcagcgccc tgaccgagcg cttctacacc ggccggacgc gcgcctgggt cgatgaggcc
     3481 atcgacctga cgccgttcgc cgggcaggaa atcctgctgc gcttcgaggt tgtcaccgac
     3541 ctcatcctca gctacaccgg cctggcgctg gacgacatcg ccatcccggc catcggtttc
     3601 gccgacgacg ccgagacgct ggacgcgggc tggctggccg agggctttgt gcgggctacg
     3661 gcgacactgc cgcagacgtg gcctttgcaa ctggtgacct tcgaccgcga cgagcagccg
     3721 accgttcagg cgctgcccgt cggggccgat ggtcgagtgc agtttgtgct ggaggcgacg
     3781 cccggcgccc ggcggccgct gctcatcgtc gccgccaccg cgccggacac gctccaaccg
     3841 gcgagctata cactgcgtgt gggtgagcca tgaccgagga cacgcgacgg gggggaggct
     3901 ggcgggagtg gatgggagtc gaacccaccg ccgctcgctc agcgcgaccg gccaacggtt
     3961 ttgaagaccg cagcatccac cgggacacaa ccactcccac gggcaattgt gcctcatgcc
     4021 cggcggttgt caatcgcccg gctgagcgca atcctcattt tacttgacag ccccggccca
     4081 tttcgataga atccctttac ctttaacagc gcagatccca ctggccggga tagctcagtt
     4141 ggtagagcac ttcactgaaa atgaaggtgt ccccagttcg agtctgggtc ctggcatcga
     4201 ttccggtcag cacgatggaa tgaatgcggg cgtggttcag tggtagaacg tctccttgcc
     4261 aaggagaagg ccacgggttc gaatcccgtc gcccgctcag gcgaaacggc aggccgcagg
     4321 gcctgccgtc ttgttatggc gacgtagcca agtggtaagg caggggtctg caaaaccccc
     4381 atcaccggtt caaatccggt cgtcgcctca gcttccccca ggcccttgcc cccgcaaggg
     4441 ccttttttgt gcgttgggtg cgccacgccg ttttcctgat agaatcggca gccgtcggcc
     4501 actaccggtg aggcgcaagt ccaccggccg gccacagcga ttcatgaccg gagaagtccc
     4561 atgtctctta cccccatacc gaatttgcct tttgccatcg acacgactag ctgggaaggt
     4621 ttccaggccc gctatgaggc gctaaccaat gagccgctca acgaggcgac gctggccgat
     4681 tggctggcgc gctggtcgca gctcaacatg ctcgtcgagg agtccggcgc catcgccaac
     4741 atcgagaaga cgctggacac ggccgacgaa acagccgagc gcgcctttct gaactttgtc
     4801 gagaacattg ccccgaactt tagccgcgcc gaccaggccc tgaagcagcg cttgctggcc
     4861 gcggcgacgg acaatgacgc cctgggcgaa gacatgcgcg tgcccctgcg ggccatgcgt
     4921 aaccaggccg acctcttccg cgaggaaaac gtgcccctca tgaccgaact ggccaagctg
     4981 ggcaacgagt acgacaagat caccggcgcg ttgtcggccg actgggatgg cgagccgcgc
     5041 aacctgaacc agctcaacgc cctcctgcgc gaccgcgacc gcgccgtgcg cgagcgggcc
     5101 tggcggcaga tgatggccct gtggctggga cagcgcgcgg cgctcaacga tctctacggg
     5161 cgcatgctgg ccctgcgccg ccagatggcc gccaacgccg gcctgcccaa ctaccgcgac
     5221 tacatttacc gcgcctataa ccgcttcgac tacacgcccg acgactgcct gcgcttccac
     5281 gacgctatcg ccgccgtcgt cgtgcccgcg gcccagcgcg tctatgagcg ccgccgcgcc
     5341 cgcctggggc tagacgccct gcgcccgtgg gatctggccg ttgaccccag cgacgccccg
     5401 ccgctggccc cctaccacgg ccaggacgag ttgatccagg gcagcctcaa catcttcgag
     5461 cgcgtcgatc ctggcctggc gcggcaattc gccctcatgg ccgaggagaa cctgctcgac
     5521 ctggacaccc gcgcgggcaa ggcgctgggc ggctattgca gcagcctcgt ctggcggcgg
     5581 cggccgtaca tcttcatgaa cggcgtcggc agccacgacg acgtgcagac gaccctgcac
     5641 gaagccggcc acgccttcca cgccttcgag tccttcgccc tgcccaccat ctggcagatg
     5701 gaagtgccgc tggagttctg cgaggtggcg tcgatgggca tggaactgct ggccgcgccc
     5761 tacctgaccc gggcgagcgg cggcttctac acgccggccg aggcggcccg cgcccgcagc
     5821 gagcatttgg agggcatcct caccttcttg ccctacatgg ccgtcgtcga ttctttccag
     5881 cactgggtct acacccaccc cgacgccgcc gccgacccgg ccgcctgcga cgcagcctgg
     5941 gatggtctct gggcgcgttt catgcccggc gtcgattgga gcggcttcga ggccgaacgc
     6001 gcaaccggcc ggcaccgcaa gctccacatc ttccacatac ctttctacta cattgagtac
     6061 ggcctggccc aggtgggcgc gctgcaagtg tggcgcaacg ccctgcacaa ccaggcgcag
     6121 gccgtggcca cctaccgcga cgcgctgaaa ctgggcggaa cgcgctcctt gcccgaactc
     6181 tatcgcgccg ccggggccga gttccgcttc gacgagccga tgctgcgcga cctggtcgac
     6241 ctgatcgaga cgacgctggc ggagttggag gcaacggagc cgtagcatgg acgcgcggga
     6301 gagcctggca cccagcgcgg cgatgcgcga atacctggcc gagatttacc ggctacagga
     6361 ggattcgccc acggtcagca caaccagtct ggccgaccgg ctgaacgtct cgccgccggc
     6421 catcccgcgc atgttaaagc gcttgcagag cgtcggcttt gtcaaacacg tgccctatca
     6481 gggcgtcgaa ctgaccgagc gcggccgcga ggaggcgcta cgcgagattc gccgccatcg
     6541 cctcctggaa gtctttctcg tcaacgtgat gggcttcacc tgggacgaga cccacgagca
     6601 cgccgacgac ctgagcgcgg gtctgaacga ccggctgacg gagcgcatgg ccgagatgac
     6661 cggccagccg acgcgctgcc cccatggcga accgattccc gaccgcgacg gccgcctgcc
     6721 ggaagtcaac gatatctgca tcgtcaacct gagcgtcggc caccgcggcg cggtcagccg
     6781 ggtgcgcacc cacgagccgg agaagctgcg ctacttcgcc agtctgggcc tgacgccggg
     6841 cacggccatc gagatcgtcg gccgcgcgcc cttcaacggg ccgatgcgcc tgcgcgccgg
     6901 ccgcgaagaa gtggttattg gctatgaact gatgcggtcg ctgtgggtga cgtaaacgcc
     6961 tgttgcgcca gattaccgtg tcggttgggg ggcgtcggtt agcaagcgcc accacgcctg
     7021 ccacggccgc gcctccaggg cggggtcggg cgtcggtggt ggcaaggcgg tcggctccgg
     7081 cgtggcctct tgcagcagag ggacgacgcg ccgctcgccg ggcaggatca tgccgctctc
     7141 gttgcgcgca taggcggcca cgtaatcatc gctggtcaca taggccagag tggcttgcaa
     7201 ctgcacctgg cgcgtggcct cgccctcgac ctgcacgcgc agcgcttcct cgccactgcc
     7261 caccagccgg cccgcctgcg cccggcggtt gaggtcgatg gcgatgatga tcgccccggc
     7321 gatgaccagt aggaaaatga cctgcggcac gttgagcagc ggccgggagc gaaagacctt
     7381 cgacgcgaac aacttcttgg ccatcgccgt gatcttagcc gcatccggga cggggtgcaa
     7441 gactggagat ggtaaaagaa tggtacgcgg gatagcacgc tgattgacgc tgagaagaac
     7501 gctgattttc gctgccagag atcctctttc agcgaaaatc agcgtgatat cagcgagaat
     7561 cagcgtccta ttctggacgg ttgcccggcc cgccttgccg atcgcccgcc cgccggggta
     7621 gaatgggcca atgtccgtcc ctgcgctcgc ccagccacca gttgattttc tggccgtcgg
     7681 ccacatctgc catgacctgg ttcccggcgg gcggctgacg ggcggggcgg cggcctatag
     7741 cagcctgacg gccgtggccc tcggcctgcg cgccggcgtc gtcaccagcg ccgccgccga
     7801 ggacgactgg gcggcggttt ttccgcagct ggccgtaacg caggtgcccg caccggccac
     7861 gaccgtcttt gagaacgtct acacgcccgc cgggcgcgtc cagacgctcc acgccgtcgc
     7921 cgggcggctg cggcccgaac acgttcccgc gggctggacg cgcgcgccca tcgtcctcct
     7981 cggccccatc gccaacgaaa tcgacccggc catcatccac ctcttcagcg acagcatcgt
     8041 cggcgtcggc ccgcagggct ggctgcgccg ctgggacgcg gccgggcggg tctggcccac
     8101 cgcctgggag ggcgcggccg aggtcttgcc gctggcggcg gtgaccttcc tgagcgagga
     8161 ggacttgctc gacgcggcca tgctcgacga cacccgccgg ctggcccgca tcgtcgtcct
     8221 gacccagggc gagcgcggct gcaccgtctt ctgccgcgac gaggcgcgcg ccttccccgc
     8281 gccgccggtc gatgtcgttg accccaccgg cgcgggcgac atcttcgccg cggcctacct
     8341 ggtgcgcttc taccagaccg acggcaacgc ctgggccgcg gccgagttcg ccaacgacat
     8401 cgccgcccac gccatcaccc gccgcggcct gccggccaag agccagatca tccggcagac
     8461 gctcgacgcg ctcatgcaca accccgccgc ccggtaacga tcatggccca catctacgcg
     8521 atcgcaaacc agaagggcgg tgtgggcaag acgacaaccg tcgtcaacct gactgcctat
     8581 ctggctggca gcggccggcg cgttctgctg gtcgatgtcg atccgcaggc caacgccacc
     8641 tcggccctgg gcttcgacaa gtatcagatg cagccctcga cctacgagct gctagtggag
     8701 cagaagccgg ccgccgtcgt tcgccggcgg caggcggaat ataagatcga cctgctgccg
     8761 tccagcccgt cgctggccgg ggccgaaatc gaactggtgc aggctatcgg ccgcgagtac
     8821 cgtttgcagc gcgccttgca ggccgtcgcc gacgagtacg actacatcct catcgactcg
     8881 ccgcccagtc tgggcctgct gacggtcaac gccctgaccg ccgcccgcga cggcgtgctc
     8941 atccccgtgc agtgcgaata tctgcccctg gaggggctga cgcagttgac gcagacgatc
     9001 cagctggtgc aggagtacct caacccgacg ctgggaatcc gcggcgtgat gatgaccatg
     9061 tacgatagcc gcaccaacct gagccggcag gtggtcgagg aagtccggca gcactttccc
     9121 aacaaggtct tcaagacgat cattccgcgc aacgtccgtc tgagcgaagc gcccagcttt
     9181 ggccagccga tcaatttcta cgcgccacag tcgcccggcg ggctggccta caagctgctg
     9241 gcaaccgagc tgctcaacgg cgaccggcgc aatggagggg gcgaacatgg ccgctaagcg
     9301 acaggtactc ggccgcggcc ttgacgcgct gtttgccacc ggcgaccgga cgactcgcgc
     9361 cgatgccgtc cgccaggtag cgattgacct catcgcgccc aatccccggc agccgcggac
     9421 gacactcgac gccgagaagc tgtccgaact ggccgcgtcg atcaaggccc acggcctcat
     9481 ccagccgctc atcgtcacgg agacggccga aggatatgtg ctgatcgccg gggagcgccg
     9541 ctggcgggcg tcgcggctgg ccgggctggc cgacgtgccg gtcatcgtca aagagacgac
     9601 gccgcaggac atgctggagt tggccctcat cgagaacatc cagcgcgccg acctgaatgc
     9661 cctggaagag gcccacgcct accgccagct aaccgaggag ttcggcctga cccacgaggc
     9721 cgtggccgag cgcgttggca aggcgcgcac gacagtgacc aatctgctgc ggctgctggc
     9781 cctgcccgaa cccatccagg ccgccgtcag cgacgagcgc ctcagcggcg cgcacgcccg
     9841 tgccctgctg ccgctgccca cgccggaggc gcaactggcg gccatgaacc agatcctcaa
     9901 gcttaacctc agcgtccggc agaccgaggc gctggtcagc agcctgatgg ccgacaggcg
     9961 tcccgcgccg cgcccgcgca agcagttgcc gccggagctg gccgcgctgc aagggcgctt
    10021 cgagcagacg ctgggcacgc gcgtcagcat cgagcagggt agtcagggcg gccggattgt
    10081 gatacattac tactccgacg aagaactaaa cgcgatttac gaagccatcg ccggcgagta
    10141 acccatgacg gactgagttc accccccata acctcttttg ctggccaacc atcccgccca
    10201 ccggcgggct gtggacggat cgccgcgcgg cgctccccaa gaggtgtgaa ctcatgcttg
    10261 ccgttcagcg catttccaaa tctttcggtc tgtctcccat cctcaaagac atctcctttg
    10321 ccctcggccc cggcgagcgg ctgggtctca ttggccccaa cggcggcggc aagacgacgc
    10381 tgctgcgcat tctgaccggc caggaaacgc ccgacgccgg ccacgtcgcc cggacgccgg
    10441 ccgatttgcg cgttggctac ttgccgcaag ggttgaccat tgacccgcgg gcgacgctgg
    10501 gcgatgtgct ccatgccgcc gcgcccgatg ccgggcgtct ggcggccgag ttgggcgagc
    10561 tggccgtgcg gctggcggcc gagccgggcc gcgccgatgt gcagcacgcc tacgacgatg
    10621 tgctgcaacg gctgagccgc agcgagggcg gccgggcgga gggcgtgctg gcccacctgg
    10681 gcctcgacag cctgccgcgc gagcagcccg ttggccggct cagcggcggc cagaagacgc
    10741 gcctggcgct ggccctggtg ctgctcgacg agccggacgt gctgctgctg gacgagccaa
    10801 ccaaccacct cgacatcgac atgctggagt ggctggaagc ctggctggcc aacttctccg
    10861 gcgcggccct catcgtcagc cacgaccgca ccttcctcga ccgcaccgtg acgcgcatcc
    10921 ttgatctgga cccgacgaca cagaccgtgc gcgagtacgc cggcaactac agcgactacc
    10981 tcgaccagta cctctccgcc cgcgagcggc agtacaccac ctggcgtcat caggaggacg
    11041 agatccgccg catgcgcgcc gacatcaccc gcacgcgcga gcaggcccgc cacgtggaga
    11101 tcaccacccg gcccaaccaa cccggcgtgc gccgcattgc caagaaggtg gcccgcaagg
    11161 cggcctcgcg cgagaagaag ctggagcgct acgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnngccaaga aggtggcccg caaggcggcc tcgcgcgaga agaagctgga
    11341 gcgctacgtg gccgccgacg agcgcgtcga gcggccggag cgcagttggc agatgaagct
    11401 ggccttcgac acgttgagcg aggcccatat cggccgcgac gtgctgacgc tggaagatct
    11461 aaccgtgggc tacgaccggc cgctgctggc cgggctaaac ctgtccatcc gcgccgggca
    11521 gcgcgtcgtg ttgacggggc caaacggggc gggcaagacg acgctgctgc gcaccattgc
    11581 cgggcgcgtg ccgccgctgg ccgggcgggc gcggctgggg gccagcgtgc agttgggcta
    11641 catgtcccag gagcaggagc acctcgaccc ggatgccacg ccgctggcga cgatcctcgg
    11701 cgtcgccgcc atgaccgaga ccgaggcgcg gtcgttcctc cactacttcc tgttcgaggg
    11761 ggatgacgcc ctgcgaccga ttgccgcgct cagcttcggc gaacgcagcc gcctgtcgct
    11821 ggccaggctg gtggccgcgg gtagcacctt cctgctcctc gacgagccga tcaaccacct
    11881 tgacattcct tcgcgggcgc gctttgagga agcgctgacg ggcttccccg gcacgatcct
    11941 ggctgtcgtc cacgaccgct atttcatcga gcgcttcgcc acggatgtct ggctggtgga
    12001 gggggagcgg gtgacctggc tgccgcagga ggtggagggg ttttaggggc taggggctag
    12061 cgattagggg ctagggaaaa gcgctcttcc ctagccccta gcccctagcc cctaatccct
    12121 atcccctggc ttcggccata gcctgctcca gctcttctac gcggcgctgc gattcggcgt
    12181 agcggtcgag taagtcgagc gcgccgtctt cggttgtggt gctcttctgg tagtaccaga
    12241 cgatggcggc gacgagcagg gcgataaaga agccgatgaa aaaggtcgaa cgcagtgatc
    12301 gcatgagggc ctccgtcagg cattgtccgg cgcggactgg ccgcgcagat agcgattgat
    12361 ggcctcgcgg gcggcgggca ggtgctgcat ggcccgccag tcaccgggga tggccgccgg
    12421 cccgtcgagc gccagcagca gcgcgaacgg gtcgctgtgg gtccgctcgc cggcggcgcg
    12481 agtggcctgc cagtgggggc gcagggccgc ctcccagcgc gggtagctct ggcgcagacc
    12541 aggccagacg gcgatgtact ccgcccgggc ttcggtcgcg tccatcttct gccggtagac
    12601 gccgacgacg acgcgctcca accgatccca accggcgacg aaagcggcga aatcgtcgtc
    12661 ggccgtccac tggctgagga agcgcgtgaa tgggttggta gctgctttct tcacggacgt
    12721 gattatagca tgggcgtggc tggggattag ggattgggga ttagggattg ggttccaggg
    12781 gccaggggcc aggttccagg gaaagaacgc tcttcccctg gcccctagcc cctggcccct
    12841 aacccctggc ccctggcccc tagatcgtgc cattcttctg cccgcaattg acgcgctgcc
    12901 ggtggtatgc tagactgtcg tccatgttgc ggttaacagt cggcacctct ggctccccgg
    12961 gcgcggcgcg ccatcatcat ggtgagcttc ttccacgccc cccgggttga ggttgccggc
    13021 tgccgccacg aaacagagtc accaccctcc tcagacccct ggggagggtt ttttgttgcc
    13081 ggagcgaacg atggaacgaa cggatatccg cctggccctg cccagcaaag gcatcttgca
    13141 agacggggcc atcgaattcc tggaagcgtg cgggctgaga gtcttccggc ccaacccgcg
    13201 ccagtatgcg gccaccatcc cggccctgcc cggcgtgacg gtgatgttcc agcggccggg
    13261 cgacatcgtt accagcatcc agcagggcag cgtcgagttc ggcatcagcg gctacgacgt
    13321 gctggccgag cgcggcgcga acggggacag cgccgtgctg gtcatccacg aagcgctggg
    13381 cttcgggccg tgcgccctga agctggccgc gccggaggac gcgccgccgc gggacgtggc
    13441 cgagctgccc ggctgggcgg ctgagttggc cgcggccggg cggccgctgc gcgtggccac
    13501 caagttcccg cgcctgacgg cggcgttcct ggacagccat ggcatcgcgc cttatcaact
    13561 cgtcgccgtc gagggcacgc tggagatcgc cccggccatc ggctacgccg acctgatctg
    13621 cgacctggtc tcgtcgggca tcaccctgcg cgacaaccac ctgcgaccgc tggcgggcgg
    13681 cgtcgtcctg cgctcagagg cggtactcat cgccaatcgc gccgcgctac gccgtcgccc
    13741 ggaagcgctg gccaccgccc gccacctgtt ggaatacatc gaggcccacc tgcgggcgca
    13801 aggctcctac ctgctcaccg ccaacgtgcg cggcgagtcg gccgaggcca tcggccggcg
    13861 catgttcgcc cagacgacgc tgggcggcct gcaagggccg accgtcgccc cggttgtggc
    13921 ccgcgaacac ctgcgcgccg agggcggctg gtacgccgtc aacatcgtcg tgcgcaagga
    13981 gcgccttttc gaggccgtcg gcgaactacg cgccatcggc ggcagtggcg tggtcgtcgt
    14041 cccttgcgcc tacatcttcg aggaagagcc ggagcggtat agagcgatgg tggagcaggt
    14101 aggggcaggt tggtaggggc gggtccgaga cccgccccgg cgcggcgcgg caatggcggc
    14161 attggtggct gacaatggct caggaacgac aacgaacatg atcacgatct acaccacaga
    14221 cgaggctcaa cagacgattc tgcgccgcga gcgagcactg gccccggaac tgaccgcgcc
    14281 attgcgcgcg cgcatgctcg ccctcttcgg cgaggcgttg acccccgccg cggccgtggc
    14341 ccggattctg gccgacgtgc gtgcccgcgg cgacgccgcc ctcttcgact ggacacagcg
    14401 cctcgacggc gtcgccctga cgcggctgga cgtgcccgcg gaggacatcc gcgccgcggc
    14461 ggcggcgctg ccagccgacc tgcgccagag cctgcaactg gccgccgacc gcatccgcgc
    14521 cttccacgcc ctccagccgg ttcactcgtg gacgactgga gcgctgggcg gccggctggg
    14581 ccagcgcgtc gccccgctgc ggcgcgttgg tgtctacgtg cccggcggca ccgcgccgct
    14641 gccgtcgtcg ctgctcatgt cggccatccc ggcgcaggtg gccggcgtgg ccgagatcgt
    14701 cgtcgtgaca ccgcccggcg gcgacggccg cgtgccgccg gtcatcctgg ccgcggcggc
    14761 gacggccggc gtagagacgc tctacaccct cggtggagcg caggccatcg ccgcgctggc
    14821 cttcggcacg gccagcatcg gccgggtgga caagatcgtc ggccccggca acctcttcgt
    14881 caccctggcc aagcagcagg tcttcgggca ggtgggcatc gacgggctgg ccgggccgac
    14941 ggagacgatg atcatcgccg acgaaacggc caatccggcc tggctggccg ccgacctgct
    15001 ggcccaggcc gagcacgacg cgctggccgc ggccatcctg ctgacgccct cgcgcgccct
    15061 ggccgaggcg gtgcagcgcg aggtcgcccg gcaactggag gcgctcagcc gcgacgccgt
    15121 catcgccgcc gcgctggccg ggcagggcgg catcgtcctg accgccgacc tggacgaggc
    15181 cgtggccctg gccaacgcct acgccccgga gcacctctgc gtggccacgg ccgcgccgcg
    15241 cgccctggcc gaccgcctcc acaacgccgg cggcctcttc ctgggcgagc gctccttcga
    15301 agtcctgggc gactacgtgg ccgggccgag ccacaccatg cccaccggcg gcacggcccg
    15361 cttcgcctcg ccgctcaacg tgctcgactt cgtgcgcatc accagcctga tcgaactgga
    15421 cgcggccacg acggcggcgc tggccccggc ggcggcccg
//
LOCUS       DAOSMQ010000009        15022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_3997_length_15022_cov_4.347832, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000009 DAOSMQ010000000
VERSION     DAOSMQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15022
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3997_length_15022_cov_4.347832"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK829_00710"
     CDS             <1..456
                     /locus_tag="PK829_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOU39763.1"
                     /translation="LSKADFSRAKMSWADLRNTVVTELTQIDPKWRLVWEIVNQGAMQ
                     RDLSRADLAGANLREANLNGADLRAAKLWLADLSGADLRRANLRHTDLRESNLVGADL
                     RETNLQDTNLTGARYDSQTRWPAGFDPLSSGALARSTVARPETLREPIP"
     gene            503..1174
                     /locus_tag="PK829_00715"
     CDS             503..1174
                     /locus_tag="PK829_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOU39764.1"
                     /translation="MCGRFALVATGEEVATHYELAEVPFIAPRYNVAPTQPVAAVRLD
                     RNGARELTHFQWGLIPSWSKDPAMGAKLINARAETAADKPAFRAAFKRRRCLIPATGF
                     YEWQTMGKGKQPMYVREAGGGLLSLAGLWEIWHAPDGGELETCTILTTLPNTLMEPIH
                     NRMPVILEPADYAMWLDPATEMDQLNHLLRPFAPEGLAAYPVSTAVNRVQNDSEACIA
                     PLAQE"
     gene            complement(1314..2441)
                     /locus_tag="PK829_00720"
     CDS             complement(1314..2441)
                     /locus_tag="PK829_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU39765.1"
                     /translation="MTTKRALSLLLLLPLLLLATQVARADTIHVVQPGDTLWTISQDY
                     GVSVEAIAEANNLPNIRAVMLGYPLIIPGVSGPLGAPTPTSTPRPAATPRTYTIPASA
                     VATDGGVIHTVAAGDTLFSISMTYNILMSAIIDANDLADPRHIALGSKLFIPGATLNR
                     PQATATPRPAATPASGQSAAPASGNLFVNGNFEGDWYFYLYNELQVPEGWLLATDEGP
                     NTLEGGPGGNFNRPEVRVVGTNQLPEAEWPLFVFNGTKTVKAFKGGAPTAFSMFQDVY
                     LQPGRYRMTINFFPDIVADYNRGGERIFNTDPLAGEARIIHNDGGTWFEPVTAGQKNT
                     RVYEFTVSQAGSVRLGASFRNRFETANNGWFLDDWRLEKIG"
     gene            2561..3058
                     /locus_tag="PK829_00725"
     CDS             2561..3058
                     /locus_tag="PK829_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39766.1"
                     /translation="MTQPVVNPEVRQAGKRPFGLYAIMVLLLLTIFAAALDILRLRSG
                     LATPLLLQLADIVSESASLSTLPPLVFSDRNLLLLSNVVIILVILLTVIGLWFRLWGA
                     WIMAMLLIGLGLGYNIWKYLEGAPFYLSMFIHVVSVFYMNERSVRQVFERPTPTAPPS
                     GTMWS"
     gene            3055..4851
                     /locus_tag="PK829_00730"
     CDS             3055..4851
                     /locus_tag="PK829_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39767.1"
                     /translation="MSDLDLLRQYEPILRLTQGELFFPRAVDGYVAASSLWELTADKR
                     PVERAPEGTLTTDKLATYDTLPPGHVFYLRFVREPLSPLEYQRWLARPDRPAFKAPGR
                     LARVPLISRIGDAFFNLSLLLRGTLPGGTSAAAEIKTRELNRLDDRAVYYGRVVRDGG
                     WIVLHYLYFYTMNNWRSGFYGVNDHEADWEQAFIYLYEQDGATRPMWTAYAAHDLRGD
                     ELRRRWDDPLLVKEGNHPVIFAGAGSHGSYFEPGEYMMAIEPAFLTPVKNALNAARKF
                     WTETLRQGDPAAAAQRIERLLSVPFVDYARGDGPSIGPGQARPWSPVLIDDRVHWVDR
                     YRGLWGLDTQDRFGGERAPSGPKFNRDGSIRMSWYDPLGWAGVDKLFPVGRLDDEVRT
                     RIDEVTAELEGVRQQIQTERALLRDVALDVEALKATAYFSGLREARARELATIQSTFQ
                     ALVRRESELTETRLALSDYWGRIQVGDYGPPDAHLRQSHHPAPPAEQRQAIEIWAAVS
                     GALALMTFGYLIVTTPEHWIAWALAVATAFGAIDALARGRVARFLLSVIIVLAVVCAI
                     ILFIEFWQWIIIGGLAVIVIYMIRDNLRELRA"
     gene            complement(4812..5675)
                     /locus_tag="PK829_00735"
     CDS             complement(4812..5675)
                     /locus_tag="PK829_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39768.1"
                     /translation="MNRFGLLLLMLAMALLAVGCDASDPPPPAATTAATAPATALLPT
                     VAATATATAPVETLPPAPSPTAAAQATPEATRTGADYVLRLPREDAVPSGWAMSPVPD
                     FETRAPRPGETYHFACRDLPARSIGVASVGYRHLDGLPSVSIEYVIYPTAEDAAARMS
                     ARAAADGCDDFTIGSGDAATTASLALLDTPTSGEEGFAAALTTSSVVTGDLVTHVVKV
                     RQNHIIIGISHTARAGEAPPDETLTRALIETALDNLHDGPLPPAVRPTGPSAARPAPY
                     ALSSRRLSRIM"
     gene            5930..6340
                     /locus_tag="PK829_00740"
     CDS             5930..6340
                     /locus_tag="PK829_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39769.1"
                     /translation="MTEHVEMMNVKTLTDKVVEFGRESLYMSLGVVDVVQENVQEWIK
                     RGMDYRHTLVERGEKMAQENRGRVNELVEMPQTVAKDTVKKANDTLDKYSEQVLTRVH
                     VPTVEQIENMTKKVGSVERKLDKVIKENAAAQQI"
     gene            6452..7195
                     /locus_tag="PK829_00745"
     CDS             6452..7195
                     /locus_tag="PK829_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU39770.1"
                     /translation="MLDRNQSRRTARARILVVDDNGPERDGMCQALETAGFRTDCVIS
                     AEEGRRHVGRFGSPDLLLLALRPQSHAAEVALCRDIHATGDVPLIALTTAAAHSEATL
                     LETCFDDFITRPAEPSEIVMRVRRCLRRWATPGEGSEDGAGRHATGQAVEIAGHTVML
                     TDREDTLLRLLMRYPNRALSRAYLLQQVWPDEPVNDGTLRVTIHRLRRKLEQTVGQRG
                     VIVSVRGSGYLYALDARPAADPAPKRAIA"
     gene            7257..7793
                     /locus_tag="PK829_00750"
     CDS             7257..7793
                     /locus_tag="PK829_00750"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU39771.1"
                     /translation="MTHWLNKQDIYDGKIIRVSRAETRLAGQVQAFDIVSHHGGAAVV
                     PWRGDCVLLVRQQRPVVDEALLEIPAGKLESADDDPAARAQAELGEEIGYHAGRLVPV
                     ATFYVSPGYCTERIAVFLGLDLEPTEANPEATEDIEIVEMPLDEARQMLDEGRFRDAK
                     TIIGLRELLAYLAREEQL"
     gene            7790..8479
                     /locus_tag="PK829_00755"
     CDS             7790..8479
                     /locus_tag="PK829_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39772.1"
                     /translation="MSDAPRPARRVPTEFDWIIYADATFAGLSLLLPLPFVDSLIETY
                     FRRRMPRDIARRRGRALPGQSIRAVNRQRATLFPGCLLWPVQAIIYLIRNIWRTVLYV
                     LTIVDASEKLSYYWHRAFLLDYAIGRGDLDQPARAELAATALHRVLETTQTSPLLNLA
                     QEIVAEARQRLGALARAIFGWLRRRKSSEAGQAASTIAAHWAEFRDYLIALADRYDAA
                     LAELESAPPTP"
     gene            8537..9430
                     /locus_tag="PK829_00760"
     CDS             8537..9430
                     /locus_tag="PK829_00760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU39773.1"
                     /translation="MTDPATSTVPYLERSFRVRHYECDAYGHVNHANYLRYMQEAAFD
                     ASAAAGYDIARYEADGRQWLVRETDITYLRPLVYGDTVIVRTWVADFRRVRSRRAYAL
                     RLAGSDEPMATATTEWVLLDSATLRPATIPPEMIAAFHHPGVAAGERREPFPEAPSEP
                     AGVFRTRRRVEWRDIDPAGHVNNANYLAYIEECNVGVAAAHGWPMARMMAANLGIVAR
                     RYRIEYRQPALMDDELEVTTYISDVKRATAVRHNTIRRVADGALLARAHALWVFVDLA
                     TGRPRRIPADFMADFRPNVAE"
     gene            complement(9557..12307)
                     /locus_tag="PK829_00765"
     CDS             complement(9557..12307)
                     /locus_tag="PK829_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016871929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOU39774.1"
                     /translation="MSVQWYQLSAEAALQEMGSASDGLSAGEATRRLEQYGANELREK
                     AHRTKWQILLDQFKGILTVLLIVAALVSAFLGDWIEAVAILIIVVLNGVLGYTQESRA
                     EQSMEALKRMSVPIVRVRRDGQLREISARDLVPGDIVILETGNIVPADGRVLSSVNLR
                     VEEAALTGESEAVDKESDLIFEDDRALGDRRNMLYSGTIVNYGRGEFIVTGTGMETEL
                     GHIATMLQSVVEDQTPLQGRLDRMGRWLAYGALVLVAVVIGLGLLRGERNWENLLLTG
                     VSLAVAAVPEALTAVVTIALSLGAQRMLKRQALIRRLPAVETLGSVTVICSDKTGTLT
                     LNRMTVQVADLANHAFRFVHGSDDDIKVEKLSGEIEGDRTSALELLLIGAALNSDASL
                     VTEGDGSVVALGDPTEAALVHAAALLDLAKPELDRVFPRVAEVPFDSTRKRMTTLHRM
                     PKATVAMSPALQSSWEARDIPFASSPPYIAWTKGAIDGLLPLSSHVWVEGQLEPFTEE
                     WRQRIMASHDALAAKGMRVLGVALRPWEHEPKETTAETMERELTLVGMVGMIDPPRPE
                     VRDAVAECRAAGIRPVMITGDHPLTARHIARQIGITDNDLFLTGQELDRLSPSELEAR
                     AREVSVFARVSPEHKLKLIDIYQRQDNIVSMTGDGVNDAPALKKADIGVAMGITGTDV
                     AKGAAEMVLLDDNFATIVAAVEEGRIIYDNIRRFIKYLLTCNVSEIAVMLIGPLLGMP
                     LPLLPLQILWMNLVTDGLPALALSIEPPEKNVMQRPPYSATESVFGRGMLQFIGIFGI
                     VLSIIAIGSSFELWSINDEGWQTALFATLVFAQLAMALEVRSEEESLLSTGLLTNKPM
                     VGAIVITVALQLVLIYWGPAQRIFHTVALSARDLALCFGLALLVIIVVEIWKFFVRRR
                     RK"
     gene            complement(12335..14098)
                     /locus_tag="PK829_00770"
     CDS             complement(12335..14098)
                     /locus_tag="PK829_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39775.1"
                     /translation="MSSGSDYRRVLAADQFHRARRQAAIESLLARLSGQSTDLLSYEQ
                     VIGQLGVSGQSPLGTREIAIKDIVGSVGRYHDFTRTFLPRLSSDEDRWVSVGAAAPAV
                     GDLPPIDVYKVGDNYFVLDGNHRVSIARRQGLSHIDACVIEVRTRVPLPPGGRPDDLI
                     IAAEYAAFLQYTRLDLNRAGVDMRVSVPGQYGHLENAIEAYRYRLETEFGQELSFEAA
                     AARWYDEAYLPIVVAIRQQGILRYFPGRTETDFFVWLSRHHAALEQALGQHISPEVAV
                     ARLLPGVQAAERAASPRPSPLRRITHLVAPDRPAPPTSWAAERMLARYGDRLFANVLA
                     AVSPGTVTADDAAAVIQRAAGISRMERAHLTVLATPAPEESDAALLKLQTLLDEERET
                     PGLSAEMASASGGDTARRVLELAFLNDLIVLNRDYARAPGDEASPSAAARRILSQAFR
                     PILFVGPPGQPEPRRALLVHDTRRRFDEAVFLAAYVAELWGIRLTGLPISNGRNTAGH
                     VARTADYLALHEVSAAFLEPARATATLPERIVAAASESAADLIILPGPGAGQGSNRYP
                     RLDETIITALRSWPQAVLVAC"
     gene            14201..>15022
                     /gene="purH"
                     /locus_tag="PK829_00775"
     CDS             14201..>15022
                     /gene="purH"
                     /locus_tag="PK829_00775"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOU39776.1"
                     /translation="MTKRALLSVHDKNGLVALGRGLNDLGWELVASGGTAEALRQAGL
                     PVRDVAELTGAPEMLGGRVKTLHPAIHGGILARATAADDAELLARNIAPIDLVAVNLY
                     PFVETIARPAADLAAAVEQIDIGGVALLRAAAKNFARVVVLSDPADYAPALAALAEGG
                     PDEATRRALAARAFALTRAYDAAIAAYLAAPGAPADDPLPEYVHLALHRVRRLRYGEN
                     PHQQAALYAADPTAGPLGGRLVAGKELSYNNLLDLNAALEAAEAFTAPAVVIVKHL"
BASE COUNT         2580 a         4851 c         5074 g         2517 t
ORIGIN      
        1 ctcagcaagg ccgacttcag ccgggccaag atgagttggg ctgacctgcg caatacggtt
       61 gtgaccgaac tgacgcagat cgaccccaaa tggcggctgg tctgggagat cgtcaatcag
      121 ggggcgatgc agcgtgacct gagccgggcc gacctggccg gggcgaatct gcgcgaggct
      181 aacctcaacg gcgcagacct gcgcgcggcc aagctctggc tggccgacct gagtggcgcg
      241 gacctgcgcc gcgccaatct gcgtcacacc gacctgcgtg agtccaacct cgtcggcgcg
      301 gacctgcgcg agacgaactt gcaggacacc aacctgaccg gcgcgcgcta tgacagccag
      361 acgcgctggc cggctggctt tgatccgctc tcatccgggg cattggctcg ttccaccgtc
      421 gcccgcccgg aaaccctgcg cgagccgatt ccctaaccgg agcggcccgc gcccaacgcg
      481 gcgacttcaa aaggagtaaa acatgtgcgg ccgatttgcc ctggttgcca ccggtgaaga
      541 ggtggcaacc cattacgagt tggcggaagt cccattcatc gctccacgtt acaacgtcgc
      601 ccccacacag cctgtcgccg ctgtccggct cgaccggaac ggcgcgcgcg aactgaccca
      661 tttccaatgg ggcctcatcc catcgtggtc gaaagacccg gcgatgggcg cgaagttgat
      721 caacgcccgc gccgagacgg cagccgacaa gccggccttt cgcgccgcct tcaagcgccg
      781 ccgctgcctg atcccggcga caggcttcta tgagtggcag acgatgggta agggcaagca
      841 gcccatgtac gtccgcgagg cgggcggcgg cttgctgtcg ctggccggcc tgtgggagat
      901 ctggcacgcg cccgacggcg gcgaactgga aacgtgcacc atcctgacga cgctgcccaa
      961 cacgcttatg gagccgatcc acaaccgtat gcccgttatt ctggagccgg ccgactatgc
     1021 catgtggctc gacccggcca ccgagatgga tcagttgaac catctgctgc ggccgttcgc
     1081 gccggaggga ctggccgcct atccggtgag cacggcggtc aatcgcgtgc agaacgacag
     1141 cgaggcctgc atcgccccgc tggcgcagga gtagtagttc agtgcctggc actttctgaa
     1201 gtgcctggca ctttcttgaa ggccacgccg aaggtgcgac gcaccggcac aagaaaagag
     1261 agcaccggcg gcggtgctct ctttgtatca cgaaccgatt cgcgctgcgg ccgttagccg
     1321 atcttctcca agcgccagtc gtcgaggaac cagccgttgt tggctgtctc gaagcggttg
     1381 cggaacgacg cgcccagacg cacggagcca gcctggctga cggtgaactc atagacgcgg
     1441 gtgttctttt gtccggcggt gaccggctcg aaccacgtac cgccgtcatt gtggatgatg
     1501 cgcgcctcac cggccagcgg gtcggtgttg aagatgcgct cgccgccgcg gttgtagtcg
     1561 gcaacgatgt cggggaagaa gttgatcgtc atccgatagc ggcccggctg caaatagaca
     1621 tcctggaaca tgctaaaggc tgttggcgcg ccgcctttga aggccttgac cgtcttggtg
     1681 ccgttgaaaa cgaacagcgg ccactccgct tcgggcagct gatttgtgcc cacgacgcgc
     1741 acctccggcc ggttaaagtt gccaccgggg ccgccttcca gggtgtttgg cccctcgtcg
     1801 gtggccagca gccagccctc aggaacctgt agctcgttgt agagatagaa gtaccagtcg
     1861 ccctcgaagt tgccgttgac aaagaggttg ccgctggcgg gcgcagccga ctggccggag
     1921 gcgggcgtgg ctgcggggcg gggggtggcc gtggcctgcg gccggttcag cgtcgcgccc
     1981 gggataaaca actttgaccc cagggcaatg tgccgcgggt cagccagatc gttggcgtcg
     2041 atgatggccg acatcaggat gttgtaggtc atcgagatgc taaagagcgt gtcgcccgca
     2101 gcgacggtgt ggatgacgcc gccgtcagtg gccacagcgc tggccgggat cgtgtaggtg
     2161 cgcggggtgg ccgcggggcg cggcgtggac gtcggcgtcg gcgcgcccaa agggccactg
     2221 acaccgggga tgatcagcgg gtagccgagc atgacggcgc ggatgttggg caggttgttg
     2281 gcctcggcaa tggcctcaac agacacaccg tagtcctgtg aaatggtcca aagcgtgtcg
     2341 cccggttgga cgacgtggat cgtgtcggcg cgcgcgacct gcgtggccaa cagcaagagg
     2401 ggtaacagca gcaaaaggga taatgctcgt ttcgttgtca ttgttgtgtt ccgatggttg
     2461 tgattacaaa cggagaattt tggcacaacg ggcgcggcaa tgcaaatccg cgagcgtgtt
     2521 tcttgtgcct ccccacccac atacggtaac attcctcgtg atgacacaac cggtggtcaa
     2581 tcccgaagta agacaggcag gcaagcggcc gttcggtctc tatgccatca tggtgctgct
     2641 gttgctgact atctttgccg cggccctcga cattttgcgg ctccgttctg gtctggcgac
     2701 gccactgctg ttgcagttgg ccgacattgt cagcgagagc gccagcttgt ctaccttgcc
     2761 gccgctggta ttcagcgatc ggaatctact gctgctgagt aatgttgtca ttatcctggt
     2821 catcctgctg acggtcattg gcctgtggtt tcgcctgtgg ggcgcgtgga ttatggccat
     2881 gctgctcatt ggcctgggcc tgggttacaa catctggaaa tatttggagg gcgcgccgtt
     2941 ctatctgagc atgttcatcc acgtcgtctc cgtcttttat atgaacgaac gcagcgtccg
     3001 gcaggtattt gagcggccga cgccgaccgc gccaccctcc ggaaccatgt ggtcatgagc
     3061 gacctcgacc tgttgcggca gtatgagccg atcctgcgtc tgacccaggg ggagttgttt
     3121 ttcccgcgtg ccgttgatgg ctacgtggcc gcgtcgagct tgtgggaact cacggccgac
     3181 aagcgtccgg tcgaacgagc cccggagggc acgctgacga ccgacaagct ggcgacctat
     3241 gacaccctgc cgcccggtca tgtcttctac ctgcgctttg tgcgcgaacc gctgtcgccg
     3301 ctggaatatc agcgctggct ggcccgcccc gaccggccgg ccttcaaagc acccggtcgc
     3361 ctggcgcgcg tgccgctcat ttcccgcatc ggcgacgctt tcttcaacct gtcgctgctc
     3421 ctgcgaggca cgctgcccgg cggcacgtcg gccgcggcgg aaatcaagac gcgcgaactg
     3481 aatcgcctgg atgaccgtgc cgtttactat ggccgcgtcg tgcgtgatgg cggctggatc
     3541 gtcctccact atctgtactt ctacacgatg aacaactggc gctccggctt ctatggtgtc
     3601 aatgaccatg aggcggactg ggaacaggcg tttatctacc tctatgagca ggacggagcc
     3661 acccggccga tgtggactgc ctatgccgcc cacgacctgc gcggcgatga gctccgccgc
     3721 cgctgggatg accctctgct ggttaaagag ggcaaccacc cggttatctt cgccggcgcg
     3781 ggttctcacg gcagttattt tgagccgggc gagtacatga tggccatcga gccggccttc
     3841 ctgacaccgg tgaagaatgc gctgaacgcc gcgcgcaagt tctggacgga gacactgcgc
     3901 cagggcgacc cagccgcggc cgcccagcgt attgaacggc tgctgagcgt gcccttcgtc
     3961 gactatgccc gcggcgacgg ccccagcatc ggccccggcc aggcgcggcc ctggtcgccc
     4021 gtcctcattg atgatcgcgt ccactgggtt gaccgttacc gcggcctgtg ggggctggat
     4081 acgcaagacc gcttcggcgg tgagcgcgct ccctccggcc cgaagtttaa ccgcgacggc
     4141 agtatccgca tgtcgtggta cgatcccctc ggctgggcgg gcgtggacaa gcttttcccg
     4201 gtcggccggc tggacgacga ggtgcggacg cgcatcgacg aagttacggc cgagttagag
     4261 ggcgtgcgcc agcagattca gacggagcgc gctctgctgc gcgacgtggc tctggacgtc
     4321 gaggcgctca aggcaacggc gtacttcagc ggcctgcgcg aggcgcgcgc gcgcgaactg
     4381 gccacgattc agtccacctt ccaggcgctg gtgcggcgcg agtcggaatt gaccgagacg
     4441 cgcctggccc tgagtgacta ttggggacgc atccaggtgg gcgactacgg cccgcccgac
     4501 gcccacctgc gacaatcgca ccaccccgcg ccgccggcgg agcagcgcca ggccattgag
     4561 atatgggccg ctgtcagcgg cgcgctggcg ctaatgacct tcggctacct gatcgtgacc
     4621 acgccagagc actggatcgc ctgggcgctg gcggtggcga cggccttcgg ggccattgat
     4681 gcgctggcgc gcgggcgcgt ggcgcggttt ctgctgtcgg tcatcatcgt gctggccgtc
     4741 gtctgcgcca ttattctgtt catcgagttc tggcagtgga tcatcatcgg cggcctggcc
     4801 gtcattgtca tctacatgat ccgcgataac ctgcgcgaac tgagagcata gggagcgggc
     4861 cgggcggcgc tggggccggt gggccggaca gcggggggga gcgggccgtc gtggaggttg
     4921 tccagcgctg tctcaatgag cgcgcgggtt aatgtctcgt cgggcggggc ttcgccggcg
     4981 cgggcggtgt ggctgatgcc gatgatgatg tggttctggc gtaccttgac gacgtgggtc
     5041 accaggtcgc cagtgacgac gctcgacgtg gtgagcgcgg cggcaaagcc ctcctctcca
     5101 ctggtgggcg tgtccagtag cgccaggctg gccgttgtgg cggcatcgcc cgaaccgatg
     5161 gtgaagtcgt cgcagccatc ggccgccgct ctggctgaca tgcgcgcggc ggcatcttcg
     5221 gccgtggggt agatcacgta ttcgattgac acactgggca gcccgtctaa atgtcgatag
     5281 ccaacgctgg ccacgccgat gctgcgcgcg ggcaggtcgc ggcaggcgaa gtggtaggtc
     5341 tcgcccggcc ggggcgcgcg cgtctcgaag tcgggcacag gactcatcgc ccagccagag
     5401 ggcacggcat cctcgcgcgg caggcgcagc acgtagtcgg ccccggtgcg cgtggcctcc
     5461 ggcgtggcct gcgcggcggc ggtaggagag ggcgcggggg ggagcgtctc cacgggcgcg
     5521 gtggcggtgg ccgtagcggc gacggtgggc agcagagcag tcgcgggtgc ggtggccgcg
     5581 gtcgtggcgg cgggtggcgg cgggtcactc gcgtcgcagc caacagccaa cagtgccatc
     5641 gccagcatca gcagcagtag accaaaccga ttcatgggtg tttctccaac tgtcgtcttg
     5701 ggtgggccga gtataggggt atgcccggca gcgatcaatg gcaattcccc gtggggactt
     5761 gtccctgcgg ctgacctgta ctaccatttg ttacagattc acgtcattcc cgcgtctgac
     5821 caaagtgccg cttgacaaag atgacgcaat gcgttatagt atccatgact atgacgcatt
     5881 gcgttagaaa taaatattag tgctcaatgg cacaatgagg aaagcaagaa tgaccgagca
     5941 tgttgagatg atgaatgtaa agaccctgac cgataaggtc gtggaatttg gccgggagag
     6001 cctgtacatg agcctaggcg tcgtcgacgt cgtgcaggag aacgtgcagg agtggattaa
     6061 gcgcgggatg gactatcgcc acacgctggt ggagcggggc gagaagatgg cccaggagaa
     6121 tcgcggccgg gtcaacgagc tggtggaaat gccccagaca gtggccaagg acaccgtgaa
     6181 gaaggccaac gacactctgg acaagtatag cgagcaggtg ctcactcgcg tccacgtccc
     6241 gaccgttgag cagatcgaga acatgaccaa gaaggtcggc tcggtcgagc gcaagctgga
     6301 caaggtcatc aaggaaaacg ccgccgcgca gcagatctaa tcaatgccgc ctgctgtccg
     6361 ttcctctgcc gggcagccat cctcctcttc ggaaccgcaa agcccggcgc gtgatgcgct
     6421 gggctttgct gcttacatgg agtgatgagg tatgttggac aggaaccaat cgaggcgaac
     6481 tgccagggcg cgtatcctgg tggtggacga caatgggcca gagcgcgacg gcatgtgcca
     6541 ggcgctggag acggccggct ttcggaccga ctgcgttatc tcggctgagg aagggcggcg
     6601 gcacgtcggc cgctttggct cgcctgacct gctgctcctg gccctgcgcc cgcagagcca
     6661 tgccgccgag gtggcccttt gccgcgacat ccacgccacc ggcgacgttc ccctcatcgc
     6721 cctgacgaca gcagcagcgc acagcgaagc cacgttgctg gaaacctgct tcgacgactt
     6781 catcacccgg ccggccgagc cgtcggagat cgtcatgcgc gtgcggcgct gtctgcggcg
     6841 ttgggcaacg ccaggcgagg ggagcgaaga cggggccggc cgccacgcca ccggccaggc
     6901 tgtcgaaatt gccggacaca cagtgatgct gaccgaccgc gaggataccc tgctgcggtt
     6961 gctgatgcgc taccccaacc gggcgctcag ccgcgcctat ctcctccagc aggtctggcc
     7021 cgacgaaccg gttaatgacg gcacgctgcg cgtcaccatt catcgtctgc ggcgcaagct
     7081 ggagcagacg gtgggccaac ggggcgtgat cgtctccgta aggggcagtg gctacctgta
     7141 cgctctagac gcgcggccgg cggccgaccc ggccccgaag cgagcgattg cctgattggc
     7201 gcggctgccg taccattcgg gccaatacca tcgccccaat cgggagtggc aggcgcatga
     7261 cccactggct aaacaagcaa gacatctacg acggaaagat catccgcgtc agccgcgccg
     7321 aaacgcgact ggccggccag gtgcaggcgt tcgatattgt gagccaccac ggcggcgcgg
     7381 ccgttgtgcc ctggcgcggc gactgcgtac tgctggtgcg ccagcagcgg ccggttgtcg
     7441 atgaggccct gctggagatt cccgccggca agttggagag cgctgacgac gaccccgccg
     7501 cccgcgccca ggccgagctg ggcgaagaga tcggctatca cgccgggcgg ctggtgcctg
     7561 tggcgacctt ctatgtatcc cccggctact gcaccgagcg catcgccgtc tttctggggc
     7621 tagacctgga gccaaccgag gccaatccag aagccaccga ggacatcgag atcgtcgaga
     7681 tgccgctcga cgaggcccgg cagatgctcg acgaggggcg cttccgcgac gccaagacca
     7741 tcatcggtct gcgtgaactg ctggcctacc tggcgcggga ggagcagctg tgagcgacgc
     7801 gcccaggccg gcccgccgcg tgccaaccga gttcgactgg atcatctacg ccgatgccac
     7861 cttcgccggg ctgtcgctgt tgctgcccct ccctttcgtt gatagcctga tcgagaccta
     7921 cttccgccgg cgcatgcccc gcgacatcgc ccggcggcgc gggcgcgcct tgccggggca
     7981 atccatccgc gccgtcaacc gccagcgggc gacgctgttt cccggctgtc tattgtggcc
     8041 ggtgcaggcg atcatctacc tcatccgcaa catctggcgg acggtgctct atgtgctgac
     8101 catcgtcgac gccagcgaga agctgagcta ctattggcat cgcgccttcc tgctcgacta
     8161 cgccatcggc cgcggcgacc tggatcagcc ggcgcgcgcg gagttggccg cgacggcgct
     8221 gcaccgcgtg ctggagacga cgcagaccag cccgctgctc aacctggctc aagagatcgt
     8281 cgccgaagcg cggcaacggc tgggcgcatt ggcgcgggcc atcttcggct ggctgcggcg
     8341 gcgcaagtcg tccgaggccg gtcaggcggc cagcaccatc gccgcccact gggccgagtt
     8401 ccgcgactac ctgatcgctc tggccgatcg ctacgacgcg gccctggccg agctggagag
     8461 cgctccgccc acaccgtagt gatgagcgaa agagagcgca cgcccgggcg tgagtcccac
     8521 tgacaagcag ccatctatga cagacccggc gacttcaact gtcccttacc tggaacggtc
     8581 gtttcgcgtg cggcattatg agtgcgacgc ctatggccac gtcaaccacg ccaactacct
     8641 gcgctacatg caggaggccg ccttcgacgc ctcggccgct gccggctacg acatagcccg
     8701 ctacgaggcc gacggccgcc aatggctggt acgcgaaacg gacatcacct atctgcgccc
     8761 gctggtctat ggcgacacgg tcatcgtgcg gacgtgggtg gccgacttcc gccgcgtgcg
     8821 ctcgcggcgc gcctatgccc tccggctggc cggcagcgac gagccgatgg ccacggccac
     8881 aaccgagtgg gtgttgctgg acagcgccac gttgcgcccg gccaccatcc cgccggaaat
     8941 gatcgccgcc ttccaccacc ccggcgtggc cgccggcgag cggcgcgaac cgtttcccga
     9001 agcgccatcg gagccggcgg gcgtctttcg cacgcgccgc cgcgtggagt ggcgcgacat
     9061 cgacccggcc ggacacgtca acaacgccaa ctacctggcc tacatcgagg agtgcaacgt
     9121 cggcgtggcc gcggcccacg gctggccgat ggcgcggatg atggccgcga acctgggcat
     9181 cgtcgcccgg cgctaccgca tcgagtaccg ccagccggcg ctgatggacg atgagttgga
     9241 ggtgacgact tacatctccg acgtgaagcg ggccacggcc gtgcgtcaca acaccatccg
     9301 gcgcgtggcc gacggcgcgc tgctggcccg cgcccacgcg ctgtgggtgt tcgtcgacct
     9361 ggccacgggc cggccgcggc gcatcccggc cgacttcatg gccgacttcc ggcccaacgt
     9421 ggccgaataa gagaagaggt taacgccaag acgccaggac gccagggcgc aacgagcaaa
     9481 gaaaacgcag gacgccagga cgccagcgaa gatacgtggc gtcctggcgt cctgcgcgcc
     9541 ttagcgttaa gttctcttac ttccgccgcc ggcggacaaa gaacttccag atctcgacga
     9601 cgatgatgac cagaagcgcc agcccgaagc acagcgccag gtcgcgcgcg gacagggcga
     9661 cggtgtggaa gatacgctgc gccggccccc agtagatcag caccagttgc agggccaccg
     9721 tgatgacgat ggcccccacc atgggcttgt tggtcagcag gccggtgctc aacagtgact
     9781 cctcttcaga gcgcacttcc aaagccatcg ccaattgggc aaagacgagc gtggcaaaga
     9841 gcgccgtctg ccagccttcg tcgttgatgc tccacagctc aaacgatgag ccgatggcga
     9901 tgatactaag cacgatgccg aaaataccga tgaactggag catgccgcgg ccaaagacgc
     9961 tctctgtggc cgagtatggc ggccgttgca tgacgttctt ctccggcggt tcgatgctga
    10021 gagccagcgc gggcaagccg tcggtgacca ggttcatcca caggatttgc agcggcagca
    10081 agggcagggg catacccaac agcggcccaa tgagcatcac ggcgatctcg ctgacgttgc
    10141 aggtcagcag atacttgatg aaccgccgga tgttgtcgta gatgatgcgc ccctcttcaa
    10201 ccgcggcgac gatggtggcg aagttgtcgt cgagcagcac catctcggcc gcgcccttgg
    10261 ccacgtcggt gccggtgatg cccatggcca cgccaatgtc ggccttcttc agcgccgggg
    10321 cgtcgttgac gccgtcgccg gtcatggaga cgatgttgtc ctgacgctga tagatgtcga
    10381 tgagcttgag cttgtgctcc ggcgagacgc gggcgaagac gctgacctct ctggcgcgcg
    10441 cctccagttc gctgggcgag agccggtcta gctcctggcc ggtcaggaac aggtcgttat
    10501 cggtgatgcc gatctgcctg gcgatgtggc gagcggtcag gggatggtcg ccggtaatca
    10561 tgaccgggcg aatgccggcg gcgcggcact cggccacggc gtcgcgcact tccggccgcg
    10621 gcgggtcgat catgccgacc atgcccacga gcgtcagttc ccgctccatc gtctccgcgg
    10681 tcgtctcctt tggctcgtgc tcccacgggc gcaaagccac gcccagcacg cgcatgccct
    10741 tggccgccag ggcgtcgtgc gaggccatga tgcgctgccg ccattcctcc gtgaacggct
    10801 ccagttgccc ctcgacccac acgtggctgc tgaggggcaa caggccgtca atggccccct
    10861 ttgtccaggc gatatagggc ggcgaggacg caaaggggat gtcgcgcgcc tcccaggacg
    10921 attgcagcgc gggcgacatc gccaccgtcg ccttgggcat ccggtggaga gtcgtcatgc
    10981 gcttgcgcgt cgagtcaaag ggcacctcag ccacgcgcgg aaagacgcga tccagctctg
    11041 gcttggccag gtcgagcagc gccgcggcgt gcaccagggc ggcctcggtc gggtcgccga
    11101 gggcgacaac gctgccgtcg ccctcggtca ccagcgaggc gtcgctgttg agcgccgcgc
    11161 cgatgagcag cagttccaga gccgaggtgc gatcgccctc tatttcgccg gatagcttct
    11221 caaccttaat atcgtcgtcg ctgccgtgga cgaagcggaa ggcgtggttg gccaggtcgg
    11281 ccacctgcac ggtcatgcgg ttgagcgtca gcgtgcccgt cttgtcagag caaatgaccg
    11341 tcaccgagcc gagcgtctcc acggccggca ggcgacgaat gagcgcctgg cgcttgagca
    11401 tccgttgcgc ccccagcgat agggcgatgg tgacgacggc cgtcagcgcc tccggcaccg
    11461 cggcgacggc caggctgacg ccggtgagaa gcaggttctc ccagttgcgc tcgccgcgca
    11521 gcaggcccag accgatgacg acggcgacga gcacgagcgc gccataggcc agccagcggc
    11581 ccatgcggtc gagacggcct tgcagcggcg tctggtcttc gacgaccgac tggagcatgg
    11641 tggcgatgtg gcccaactcc gtctccatgc ccgtgccggt gacgatgaac tcgccgcggc
    11701 cgtagttgac gatggtgccg ctatagagca tgttgcgccg gtcgcccagg gcgcggtcgt
    11761 cctcgaagat caggtcagac tccttgtcga ccgcctccga ctcgccggtc agggccgcct
    11821 cctcgacgcg caaattgacc gaggacagaa cgcggccgtc ggcggggaca atgttgcccg
    11881 tctccagaat gacgatatcg ccgggaacca ggtcgcgggc ggaaatctcg cgcagttggc
    11941 catcgcgccg cacacggaca atgggcacgg acatgcgctt gagcgcctcc atcgactgct
    12001 cggcgcgcga ttcctgggta tagccgagaa cgccgttcag cacgacgatg atcaaaatgg
    12061 ccacggcttc gatccagtcg cccaggaagg ccgagaccag cgcggcgacg atcagcaaca
    12121 ctgtgaggat gcccttgaac tggtcgagca ggatttgcca cttcgtgcgg tgcgcctttt
    12181 cgcgcaattc attggcgccg tactgctcca gccggcgggt ggcttcgccg gcagatagac
    12241 cgtcagacgc gctgcccatt tcttgcagcg ccgcctcggc ggataactga taccactgaa
    12301 cactcatcga ggttctctcc tgtttgttgc gccgtcagca ggcgaccaga acggcctgcg
    12361 gccaactgcg aagggcggtt atgatcgtct catcaagacg ggggtagcgg ttgctgccct
    12421 ggcccgcgcc ggggccgggc aaaataatca ggtcggcggc gctctcgctt gccgcggcga
    12481 cgatgcgttc gggcagcgtc gccgtggccc gcgccggctc caagaaggcg gcgctgactt
    12541 cgtgcagcgc cagatagtcg gccgtgcggg ccacgtggcc ggcggtgttg cggccgttgc
    12601 tgatgggcaa cccggtcagc cggatgcccc atagctcggc tacataggcg gccaggaaga
    12661 cggcctcatc gaatcggcgg cgcgtgtcgt ggacaagcag cgcgcgccgc ggctccggct
    12721 ggcccggcgg gccgacgaac agaatcggcc ggaacgcctg gctcaggatg cggcgcgcgg
    12781 ccgccgaagg agacgcctcg tcgcccggcg cgcgcgcgta gtcgcggttc agcacgatca
    12841 ggtcattaag aaaggccaac tccaacaccc gtcgggcggt gtcgccgccg ctggcgctgg
    12901 ccatctcggc gctcaggccg ggcgtctcgc gctcttcgtc tagcaacgtc tggagtttga
    12961 ggagggccgc gtcgctctct tctggcgctg gggtggccag gacggtcagg tgagcgcgct
    13021 ccatccgact gatgccggct gcccgctgga tgacggcggc cgcgtcatcg gctgtgaccg
    13081 tgccgggcga gaccgcggcc aggacgttgg cgaagaggcg gtcgccataa cgggccagca
    13141 tgcgttcggc ggcccaggaa gtcggcggcg cggggcgatc cggagcgacc aggtgggtga
    13201 tgcggcgcag cggcgagggg cggggggacg cggcgcgctc ggccgcctgg acgccaggca
    13261 acagccgggc caccgccacc tccgggctga tgtgctggcc cagagcctgc tccaacgcgg
    13321 cgtggtggcg cgacagccag acgaaaaagt cggtctccgt gcggcccggg aagtagcgca
    13381 ggatgccctg ctgccggatg gcaacgacga tgggtagata agcctcgtcg taccagcgcg
    13441 cggcggcggc ctcgaaactt agctcctggc caaactccgt ctccagacga tagcgatagg
    13501 cctcgatggc gttctccaga tggccatact gcccgggcac actgacgcgc atgtccacgc
    13561 cagcgcgatt caggtctagc cgggtgtatt gcagaaacgc ggcgtactcc gcggcgataa
    13621 tgaggtcgtc tggccgcccg ccggggggca ggggcacgcg cgtgcggact tcaatcacac
    13681 aggcgtcaat gtggctgagg ccctgtcgac gggcgatgct gacgcggtgg ttgccgtcga
    13741 ggacgaagta gttatcgccg accttgtaga cgtcaatagg cggcaagtcg ccgacggccg
    13801 gggcagccgc gccgacggac acccagcgat cctcgtccga tgagagccgg ggcaggaagg
    13861 tgcgggtgaa atcatggtaa cggccgacgc tgccgacaat gtccttgatg gcgatctcgc
    13921 gcgtgcccag gggcgattgc ccgctgacgc ctagctggcc gatgacctgc tcataggaaa
    13981 gaaggtcagt cgattggccg gatagtcggg ccagcaggct ctcaatggcc gcctgacggc
    14041 gagcacgatg gaactgatcg gcggccagca cgcgtcgata gtcacttccc gaactcatgg
    14101 tgcgcggatg atagcccaat ggcgcgcccc ggccaacaat agcagcgacc gcagaggtcg
    14161 tagaagaccc ccgcggtctg gagtgataca ctcaagggcc atgaccaaac gagcacttct
    14221 ctcggtacac gacaagaacg gcctggtggc gctggggcgc ggtctgaacg acctgggctg
    14281 ggaactcgtg gccagcggcg gcacggcgga agcgctgcgc caggccggcc tgccggtgcg
    14341 cgacgtggcc gaactgaccg gcgcgccaga aatgttgggc gggcgggtca agacgttgca
    14401 cccggccatc cacggcggca ttctggcccg cgccacggcc gccgacgacg ccgagctgtt
    14461 ggcccgcaac atcgcgccca tcgatctcgt tgccgtcaac ctctatccgt tcgtcgaaac
    14521 aatcgcccgg ccggcggctg acctggctgc ggctgtggag cagatcgaca tcggcggtgt
    14581 agctctgctg cgggcggcgg ccaagaactt cgcccgcgtg gttgtgttgt ccgacccggc
    14641 cgactatgcc ccggcgctgg cggctctggc cgagggcggg ccagacgagg ccacgcgccg
    14701 cgccctggct gcccgcgcct ttgccctgac acgcgcctac gacgcggcca tcgctgccta
    14761 cctggccgcg ccaggcgcgc cggcagacga cccgctgccg gagtatgtcc acctggccct
    14821 ccaccgcgtg cgccggctgc gctatggcga gaacccccac cagcaagcgg cgctctacgc
    14881 cgccgacccc accgccgggc cgctgggcgg gcggctggtg gccggcaagg agctatcgta
    14941 caacaacctg cttgacctga acgcggccct ggaggcggcc gaagccttca ctgcgccggc
    15001 ggtggtcatc gttaagcacc tg
//
LOCUS       DAOSMQ010000010        14953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_4020_length_14953_cov_5.244731, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000010 DAOSMQ010000000
VERSION     DAOSMQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4020_length_14953_cov_5.244731"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PK829_00780"
     CDS             complement(<1..836)
                     /locus_tag="PK829_00780"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOU39777.1"
                     /translation="MPLTIIIGAQWGDEGKGHITDRLAQDAAVVARYSGGDNAGHTVT
                     IGGEVFKLHLIPSGIIHRGVVCLIGNGAVVNPVVMLREMDALAARGLDVGPARLRLSG
                     GAHLITPGHIAMDRAHERERGRGAIGTTLRGIGPAYTDKARRIGLRAELLANPAALAA
                     ALVDHLAAKNEVLVKVYGEPPLDADAILADYAAQAARLAPHLVDGPVLLDEALARGET
                     VLAEGAQGTLLDLDHGTYPFVTSSSPTAGGAITGLGVGPRHVDRVIGVAKAFTTRVGG
                     GP"
     gene            complement(942..2030)
                     /locus_tag="PK829_00785"
     CDS             complement(942..2030)
                     /locus_tag="PK829_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOU39778.1"
                     /translation="MNQPPASTDHLIAAARQAIEANRPAEARPLLERAAHQDSGDPRP
                     WLLLAGIAATPRERRVYLAQARRLDALAGRSRPPAMPAPQPRRASRRGLWLGGLALLL
                     AGALLLAFHPVGRALLGRMAGGVSALTNGAPEPTADAAASVAVATATAIATQPTALPT
                     AAAEVIPPTPTNPPVFPTKGVDPNSGAAAGQPLPTWTPTALPTPTLAPTLTPTPTPLP
                     EPTTPPVAVDPGALSPRPAGVGETERWIDVNLSTQTLVAYEGDTPVFNTLVSSGLPQW
                     PTVTGQFRTYMKYESQTMNGYLLGYDYFLPDVPYVMYFYEDYAIHGTYWHNNFGTPMS
                     HGCVNVSTPDAGWLFNWAPVGTLVNVRY"
     gene            complement(2071..2898)
                     /locus_tag="PK829_00790"
     CDS             complement(2071..2898)
                     /locus_tag="PK829_00790"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU39779.1"
                     /translation="MQAPYGNSDAADRPFWRFVGRNLALLGFLALSAGVMLAAYFYFS
                     AATGVAEVVAAGSLEGALTAPFVKPVPPRPVTQRLAQSPGPIRIGIISGHKGNDSGSV
                     CADGLTEADVVESIAVRVIAALQIRGIAADMLDEFDARLQGYVATGLVSIHADSCDYI
                     NDLATGFKIAGSGYTDSSQLSICVEDAYRQATQMNYHAATITPHMTDYHAFREIAAGV
                     PGIIIEVGFLNLDRQILTTQPDLVAGAITDGILCYIDGIRGGVSYTPAAGAPTGPED"
     gene            complement(2942..4348)
                     /locus_tag="PK829_00795"
     CDS             complement(2942..4348)
                     /locus_tag="PK829_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOU39780.1"
                     /translation="MSEPGEPLSERELDVLRRLAVGESNKAIADGLSISPLTVKTHLR
                     NIYTKLAVSTRTEALNVALQQRLVTVVAADGQAAEDLPAEPPAVAPTLMPDQDGPTAL
                     ASPAPIPPAPITGSGSSAVTGHRWRVISLALLVLLLLTAGAFAFVQWQGGALLPAGAE
                     ATPFVEQPLGDSRWLSSRPLPAARAGRAVVALGLDIYAIGGETDGGISDEVNVFDTQS
                     LAWRRAATKPTAVAAATAAELFGEIYVPGGRLANGQPTAVVEVYSPSQDAWRRAAPLP
                     QPIAGGLALADGGFLYLIGGQGEGDTLPNVYLYDPVGDSWRPVAALPEPRSEAAGGAL
                     TGQLYVVGGSDGAAGQPSCFSYDPPRDAWSDCPDMLLPRVNAGSTVLLNKLYVIGGTA
                     GDAAYGEVYDPNTRTWTVLNAPPDVFDWRAPGVTHVETRIYAVGGRRGAALSDATLVY
                     SPLTYQTYIPAAPSDRDE"
     gene            complement(4356..5399)
                     /locus_tag="PK829_00800"
     CDS             complement(4356..5399)
                     /locus_tag="PK829_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39781.1"
                     /translation="MAESMPPAGNDADTSPSGTRRGRLARRALGLALVIVSVVLATYL
                     LVAYFAYESGRTLRSQQETTARTEQIDRQIELARQDLAAESTHMALTRLDWVLAQEPD
                     NADALALRQQVAAVENAAQATAGPLPTAEPRPTAEATPAEVITDDERLSELQAVRRLV
                     AGEQWEEALPALVSFQQRFPDYERDETNQMLFDAYLALGLSYVNTEKVELGLNYFAQA
                     ERLGSLPQEALDYRVWADLYFQGMAYSGVNWDIAADYWADLCAAAPFYRDSCARLDRA
                     LVGQGDQYAYLMDWCPALTIYQRAWNRTPTEILDNKIAEARAGCAAATAVPLTNTVPI
                     TNTAPLTPTEPGG"
     gene            complement(5401..6528)
                     /locus_tag="PK829_00805"
     CDS             complement(5401..6528)
                     /locus_tag="PK829_00805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU39782.1"
                     /translation="MRVLMLSKACLVGAYQTKLEAIARMPDVELTALVPPSWDDPDGR
                     VALERCYTDGYELLVDPIRFNGHYHTYYYPTLPRRLRQFRPDVVHIDEEPYNLATWLA
                     MRQARAVGAKTLFFTWQNLARRYPPPFSLMERQVLAASDYAIMGNREAADIWRAKGYA
                     GPYRVIPQFGVDPVLFSPPAARDAGRAFIIGSANRRLVPEKGVDLLLRAAAGLPGVWR
                     LHIAGDGPALPGLRRLAEELGIAGRVDFDGPIPSDQVPAYLRDLDVLALTSRTLPNWK
                     EQFGRVLIEAMACGVPVIGSRCGEIPHVIGDAGLIIPEEDIDALRAGLLQFMQNDELR
                     REFNRRGRQRVLEHFTQASIAAETVAIYRSLSAPGAAVPLD"
     gene            6719..8638
                     /locus_tag="PK829_00810"
     CDS             6719..8638
                     /locus_tag="PK829_00810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU39783.1"
                     /translation="MLLLAVLVAAALRFTRLGAWPPGPYRDEAYNGLDALGVLRGQLA
                     LFFPANNGREPIFIYLVALSVALLGPTTLALRLPAAVAGTLATLPAALLGRAWFGRVA
                     GLLAAFLWAITFWPVHLGRIGLRAGLLAPMLATAFWLGTRAYRERRAVLWLAAGLVYG
                     LSFYTYLAARFTPLLLLLFAAYLVATGRRARLWDAGRALWFVAGAAVAVAPLATVLLR
                     DPALLFGRAGQVSILSPEVSGGDPLGALLGNTGRALGLYLWRGDSILRHNALLSYDVV
                     LKSDNPAGRPVFDLLMAGPFLVGLGWCLWRWRRPAAAFLLLWQLVMLGPTLLAEDAPH
                     FLRAAGILPGAIFFPAIGLAQLWAWPRLPAVARRLAVVGLLAGSLALTVRDYSTYARQ
                     PDVAYLWESAAAELARAARAAPQGTTVLIDRRFTEGWPSIPFLLHGHAFTPFDPAKPL
                     PALAGPTTVFAWPYGPLDFLPGAVRPPARVAIEPGPLARGDLEPEPYALYTRYDILPG
                     PAEAPPAANFDDRYGLSAAATLDAPDALTVALEWTPGAAHSPDASPPQVFIHVLGPEG
                     MLAQHDGTIGGGLWPAANWQPLLRIDERHTLALSRPFQPAQDRIEIGLYDPATGQRLP
                     LLGASGDVVVLTPDG"
     gene            8658..9674
                     /locus_tag="PK829_00815"
     CDS             8658..9674
                     /locus_tag="PK829_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU39784.1"
                     /translation="MKAPRWLPMGLLVGALLAAGAASAAQTDPTAMPIPTPTIETPTP
                     TFAPPTPWISPTPDETGAITIIVAPGESLWVIAARAGLSLGELLALNNLTEGDVINPG
                     DVLLVARVTPPAAPTSGSPTVTPPPPTLRPTETRAEAGICLEAYDDLDRDGIHDEGEP
                     LRAGVAFTIYNTEVVVANYITDGVSEPHCIAGLLPGEYRVTRSIGPGEVLTTGGDWTL
                     SLTAGSQLRQAFGSVTGAADVAAGPAAGATALPTPANPPAATTSAPSTAPAATPAGQT
                     AAAGRFIRLDWRLAGVVILFLGGLLLLAAVLILLFRQPRVVTGGGQRPEPTNERRFHD
                     LDDL"
     gene            9714..10997
                     /locus_tag="PK829_00820"
     CDS             9714..10997
                     /locus_tag="PK829_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOU39785.1"
                     /translation="MKKIAPLLVVSLFVALLGARVAAAQQGNLLQNPGFEQPFSGGAA
                     GNWQKWSQSTPRSDEECLVAYHVLPKWNVETAAGFLREGAASQYVGNNWDTWSGGVYQ
                     TVAATPGTTYRFSFYGRGRGSNESSPAASETALQMNMRAGIDPNGSGQWNDADVVWGA
                     AGSPHDTWQQFTVSATATGNQITVFTSADWGVTGVNQCRQFLDTWVDAAELVAQTLPT
                     ATPQPTQPAPTPAPVQTSSVPTEPAAATVAATLTAATATTAPTAAPAGTATVCVNAFR
                     DANGNGLRDADEGYVAGVTLTVAQGATVIGQAVSTGQAEPLCFGNLAAGTFQVGQTLP
                     PTLETTTQPNATVDVAAGQTIALEFGSRLRTTPTAEPATPTADPAQAAATATAAAVAT
                     GAPEGGSNWLVYLGLGAILVGVALLGVLLFTLLRR"
     gene            complement(11278..11490)
                     /locus_tag="PK829_00825"
     CDS             complement(11278..11490)
                     /locus_tag="PK829_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOU39786.1"
                     /translation="MEYMVVIERGEDSYGAYVPDLPGCVAVADTKDEVLTLIQEAIVL
                     HLELMQEDGLPIPTPHSVSEYVAVAL"
     gene            complement(11496..11684)
                     /locus_tag="PK829_00830"
     CDS             complement(11496..11684)
                     /locus_tag="PK829_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOU39787.1"
                     /translation="MKVREVIKLIEEDGWYLVRIRGSHRQFRHLSKPGIVTVSGSPGK
                     DMPPGTLNSVLKQAQLKE"
     gene            11831..13483
                     /gene="pgm"
                     /locus_tag="PK829_00835"
     CDS             11831..13483
                     /gene="pgm"
                     /locus_tag="PK829_00835"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HOU39788.1"
                     /translation="MALHPLAGQPAPRERLVNVPRLVSAYYTNHPDPADPLQAVSFGT
                     SGHRGTSTGGTFNDDHIAAISQAVAEYRAGQGIAGPLFAGMDTHALSEPALATALEVF
                     AANGVHVVIQRGLDYTPTPVISHAILTWNSDRARPRADGIVITPSHNPAEDGGYKYNP
                     PEGGPADTTTTRWIQERANALLADGLRGVRRIPLRRALAADTTEERDYITPYVEDLAT
                     VVDMEAIAAARLHIGVDPMGGSGVHYWEPIAARYGLHLTVVNPYVDPTFGFMSVDHDG
                     KIRMDCSSPYAMAGLIRLKDDFDIAVGNDPDYDRHGIVTRSAGLMNPNHFLAVAIWYL
                     FQNRPAWRADAAVGKTLVSSSMIDRVAAHLGRRLAEVPVGFKYFVAGLLSGDYGFGGE
                     ESAGASFLRRDGSVWTTDKDGFIMDLLAAEILARTGRDPGEHYGDLAGRFGDPVYQRT
                     DTPVSATRKAVLANLSPELVRADTLAGEPIVAKLTRAPANDAPIGGLKVVTANGWFAA
                     RPSGTENIYKVYAESFLGREHLQQIQAEAGTIIDDAFAAAGV"
     gene            13485..14273
                     /locus_tag="PK829_00840"
     CDS             13485..14273
                     /locus_tag="PK829_00840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU39789.1"
                     /translation="MSDTFAARYGPWALVTGASSGIGRAFARRLAGRGLNVVLAARRA
                     ERLEALAGELAADCGVLACPVPVDLCRDDFLDILRAATDGLDIGLLVNSAGYSLTGPF
                     LDADPDEMLRLFNVNARAPLLLTRAFGPAMRARRRGGIVMVSSVVAFVGTPQWSLYAA
                     TKAFDLLLGEGLAAELRADGVDVLTLAPGTTRTEFLDVAGLSGRMSLSAERVVDDALG
                     HLGRSDIVVPGWFYRGGTLAMRLLPRSVNRAVFGRFLAAMRTEE"
     gene            14326..>14953
                     /locus_tag="PK829_00845"
     CDS             14326..>14953
                     /locus_tag="PK829_00845"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HOU39790.1"
                     /translation="MVVVAVVAAWPAVSQPGLLNTRGGGDSPFLLQRLHQLTTALAGG
                     HFPVRWMPDAAYGDGYPFYNYYAPLSIYIAAAFRGLGFSYVRAIHLAQLAGFIVAGLG
                     TFYLGRRWLRSDWAGLLAAVAYTVAPFHMVNVYVRGDSLAEFWAMAWYPVVLLAADGL
                     VTAQRPARRRAVALFALAYAALILSHNISALIFSPFLLVYLSLRFWNAS"
BASE COUNT         2227 a         5163 c         5143 g         2420 t
ORIGIN      
        1 aaggggccgc cgccgacgcg cgtggtgaaa gccttggcca cgccgatgac ccggtcaacg
       61 tggcgcgggc cgacgcccaa gccggtgatg gccccgccgg ccgtgggcga cgagctggtg
      121 acaaaggggt acgtgccgtg gtcgaggtcg agcagcgttc cctgcgcccc ttcggccagc
      181 accgtctcgc cacgggccag agcctcgtcg agcagtacgg gcccatcgac caggtggggg
      241 gccagccggg cggcctgggc ggcgtagtcg gccaggatgg cgtcggcgtc cagcggcggt
      301 tcgccataga ccttcaccag tacttcattc ttggcggcaa ggtgatcgac cagggccgcg
      361 gccagcgccg ccgggttggc cagcaactcc gctcgcaggc cgatgcgccg ggccttgtcg
      421 gtgtaggccg ggccgatgcc gcgcagcgtt gtgccgatgg ccccgcggcc gcgttcgcgt
      481 tcgtgggcgc gatccatggc gatgtggccg ggggtgatca ggtgtgcgcc gccgctgagc
      541 cgcagccgcg ccgggccaac gtcaaggccg cgtgccgcta gggcgtccat ctcgcgcagc
      601 atcaccaccg ggttcacgac cgcgccgttg ccgatcaggc agacgacgcc acggtggatg
      661 atgccggagg ggatcaggtg gagcttaaag acctcacccc cgatggtgac ggtgtggccg
      721 gcattgtcgc caccgctata gcgggcgaca accgccgcat cctgggccaa ccggtcggta
      781 atgtggccct tgccctcgtc gccccattgc gcgccgatga tgatcgtcag tggcatgaca
      841 tgtgctcccg tatgaaaaag cgggggagcg gggagcaggg gagcaggggg aatgaactct
      901 ttctcctcgg ctcccccgct ccccggcccc ctgctctctg cttagtatct cacgttcacg
      961 agcgtcccca ccggcgccca gttgaacagc cagcctgcgt ccggcgtgct gacgttgaca
     1021 cagccgtggc tcatgggcgt gccgaagttg ttgtgccagt aggtgccgtg gatggcgtag
     1081 tcctcgtaaa agtacatcac atagggcacg tcgggcagga agtagtcata gcccagcagg
     1141 tagccgttca tcgtctggct ttcgtacttc atgtaggtgc gaaattggcc cgtgaccgtt
     1201 ggccattggg gcaagccgct gctgaccagt gtgttgaaca ccggcgtatc gccctcatag
     1261 gccaccagcg tctgcgtgct caggttgacg tcgatccagc gctccgtctc gcccacgccg
     1321 gctggccggg gcgaaagcgc gccggggtca acggccaccg gcggcgtggt cggctctggc
     1381 agcggcgtcg gcgtgggggt cagcgtggga gccagcgttg gcgtcggcag ggcggtgggc
     1441 gtccaggtgg gcaagggttg gccggcggcc gccccgctgt tgggatcgac gcccttggtg
     1501 gggaagacgg gcgggttggt cggcgtgggc gggatgacct cagcggcggc ggtgggcaag
     1561 gcggtgggct gtgtggcaat ggccgttgcc gtggccacgg caacggacgc ggccgcgtcc
     1621 gcggtcggtt ctggcgcgcc gttggtcagg gccgagacgc ccccggccat gcgccccagc
     1681 agcgcgcggc cgacgggatg gaaggccagc agcaacgccc cggccagcaa cagcgccagc
     1741 ccgcccagcc acagaccgcg ccgggaagcg cgccgcggct gtggcgcggg catggccggc
     1801 gggcggcttc gcccggccag cgcgtccagg cggcgcgcct gagccaggta gacgcggcgc
     1861 tcgcgcggcg tcgcggcgat gccggccagc agcagccacg gccgcgggtc gccgctgtcc
     1921 tggtgggcgg cgcgttccag cagcgggcgc gcctcggccg ggcggttggc ctcgatggcc
     1981 tggcgggccg cggcgatgag atggtcggtg gaggcaggtg gttgattcat gcgttcacaa
     2041 gcctggcgga gcctgtttgc gccggggcca tcagtcctct gggcccgtgg gcgcgccggc
     2101 ggcgggcgtg taggagacgc cgccgcgaat accgtcgatg tagcacaaga tgccgtcggt
     2161 gatcgccccg gccacaaggt cgggctgcgt cgtcagaatt tggcggtcga gattcaggaa
     2221 gcccacctcg atgatgatgc ccggtacgcc ggcggcgatc tcgcggaaag cgtggtagtc
     2281 ggtcatgtgg ggcgtaatgg tagcggcgtg gtagttcatc tgcgtcgcct gccggtaggc
     2341 gtcttcgacg cagatggata gctgcgacga atcggtgtag ccggagccgg cgatcttgaa
     2401 gccggtggcc aggtcgttga tgtagtcgca tgagtcggcg tggatggaca ccaggccggt
     2461 ggccacgtac ccctgcaagc gggcgtcgaa ttcgtccagc atgtcggcgg caatgccacg
     2521 tatctgcaag gcggcaatga cgcgcacagc aatgctttca accacatccg cttccgtcag
     2581 gccgtcggcg cagacggagc cggagtcgtt gcccttgtgg ccggagatga tgccaatgcg
     2641 aatggggccg gggctttgcg ccaggcgctg ggtgacgggg cgcggcggca cgggcttgac
     2701 gaacggcgcg gtcagcgcgc cctccaggct gccggcggcg acgacttcgg ccacgcccgt
     2761 ggcggcgctg aagtagaagt aggccgccag catcaccccg gccgacaggg ccaggaagcc
     2821 cagcagggcc agattgcggc ccacaaagcg ccagaagggc cggtcggccg cgtcgctatt
     2881 gccatacgga gcctgcatta tgtcgatatt ctacacgaag cgcggccaat tggaaacccc
     2941 ctcactcgtc ccggtcggac ggcgcggcgg ggatgtaggt ctggtaggta aggggagagt
     3001 agacgagggt ggcgtcgctg agggcagcac cgcgccggcc gccgacggcg tagatgcgtg
     3061 tctccacgtg ggttacaccc ggcgcgcgcc agtcgaacac gtccggcggc gcgttgagca
     3121 cggtccaggt gcgcgtgttg gggtcgtaga cttcgccata ggcggcgtcg ccggccgtgc
     3181 cgccaatgac gtagagcttg ttgagcagca ccgtcgaacc ggcattgacg cggggcagga
     3241 gcatgtcggg gcagtcgctc catgcgtcgc gcggggggtc atagctgaag cacgatggct
     3301 gcccggccgc gccgtcgctg ccgccaacga cgtagagctg cccggtcagc gcgccgccgg
     3361 ccgcctctga ccgcggctcc ggcagcgcgg ccaccggccg ccaactgtcg cccacgggat
     3421 cgtacaggta gacgttcggc agcgtgtcgc cctcgccctg cccgccgatg aggtaaagaa
     3481 agccgccgtc ggccagggcc agcccgccgg cgatgggctg cggcagaggg gcggcgcgcc
     3541 gccaggcgtc ctgcgacggg ctatagacct caacaacagc tgtcggctgc ccgttggcca
     3601 gccgcccgcc ggggacgtag atttcgccaa acagctccgc cgccgtcgcc gcggccacgg
     3661 ctgttggctt ggtcgcggcg cgacgccagg ccaatgactg cgtgtcgaag acgttgacct
     3721 catcgctgat gccgccatcc gtctcgccgc cgatagcgta aatatccaac cccagggcaa
     3781 cgacggcccg cccggcgcgc gccgccggca gcggccggct gctcagccag cgcgagtcgc
     3841 ccagcggttg ctccacaaat ggcgtggcct cggctccggc tggcaacagc gcgccgccct
     3901 gccactggac aaaggcgaac gcccccgctg tcagcagcaa caagaccaac agcgccaggc
     3961 taattacccg ccagcggtgg cccgtgaccg ccgacgaccc ggagcctgtt atcggggccg
     4021 gagggatcgg cgcgggcgag gccagggcgg tcgggccatc ctggtcgggc atgagcgtgg
     4081 gcgcgaccgc cggcggttcg gccggcagat cctcggccgc ctggccgtcg gcggcaacca
     4141 cggtgacaag ccgctgttgc agggcgacat tcagcgcctc ggtgcgcgtg gagacagcca
     4201 gcttggtgta gatgttgcgc aggtgggtct tgaccgtcag cgggctgatc gacaagccgt
     4261 cggcgatggc cttgttggac tcgcccacag ccagacgccg cagcacatcc agttcgcgct
     4321 cgctcagagg ttcgccgggt tcggacatgg gcggtctagc ctcccggctc ggtgggcgtc
     4381 agcggggcgg tgttggtaat gggcacggtg ttggtcagcg gcacagccgt agccgcggcg
     4441 caaccggcgc gggcctcggc gatcttgttg tcgagaatct ctgtcggcgt ccggttccag
     4501 gcacgctggt agatcgtcag cgccgggcac cagtccatca gataggcata ctggtcgccc
     4561 tggcccacca gagcgcggtc gagccgggca caggagtcgc gataaaacgg cgcggcggcg
     4621 cacagatcgg cccaatagtc ggcggcgatg tcccagttga cgccggagta ggccatgccc
     4681 tggaagtaga gatcggccca gacgcgatag tcgagcgctt cctggggcag gctgcccagc
     4741 cgttcggcct gggcaaagta gttcagcccc aactcgacct tctcggtatt gacataactc
     4801 agcccgaggg cgaggtaggc atcgaagagc atctggttcg tctcgtcgcg ctcatagtcg
     4861 gggaagcgct gctggaaaga caccagcgcg ggcagcgcct cctcccactg ttccccggcc
     4921 accagacggc ggacggcctg caattcggac aggcgctcat cgtcggtaat gacttctgcg
     4981 ggcgtggcct cggccgtcgg gcgtggttcg gctgtgggca gcggcccggc ggtggcctgg
     5041 gcggcgttct ccacggcggc gacctgttgg cgcaaagcca gggcgtcagc gttgtctggc
     5101 tcctgggcca agacccaatc gaggcgcgtc agggccatgt gggtgctttc ggcggccagg
     5161 tcttgccgcg ccagctcgat ctggcggtcg atctgctcgg tgcgggcggt cgtctcctgc
     5221 tggctgcgca aggtccggcc gctttcatag gcgaagtagg ccaccaacag ataggtcgcc
     5281 aggacgacgg agacgatgac cagcgccaga cccagcgctc gccgggccag gcgtccccgt
     5341 cgtgtgcccg aaggcgatgt atcggcgtcg ttgcccgccg ggggcatgga ttcggccata
     5401 tcaatcaaga ggaaccgccg cgccgggggc gctcaggctc ctgtaaatcg ctactgtttc
     5461 cgcggcgata ctcgcctggg tgaaatgctc caacacccgt tgccggccgc gccggttgaa
     5521 ctcgcggcgc agctcgtcgt tctgcatgaa ttgtagcagc ccagcccgaa gcgcgtctat
     5581 atcctcttca ggaatgataa ggccagcgtc gccgatgacg tggggaatct cgccgcagcg
     5641 cgagccgatg accggcacgc cgcaggccat cgcctcgatc aggacgcgac cgaactgctc
     5701 cttccagttg ggcagcgtgc gcgaggttag cgccagcacg tccaggtcgc gcagataggc
     5761 gggcacctgg tcggaaggaa tggggccatc gaagtcgaca cgcccggcga tgcccagttc
     5821 ctcggccagg cggcgcaggc cgggcagcgc cggcccgtcg ccggcgatgt gcaaccgcca
     5881 gacgcccggc agcccggcgg cggcgcgcaa cagcagatcg acgcccttct ccggcaccag
     5941 acggcggttg gccgagccga tgataaatgc tcgcccggcg tcgcgggcgg ccggcggcga
     6001 gaagagcacc ggatcgacgc caaactgcgg gatgacgcgg taggggccgg catagccctt
     6061 ggcccgccag atgtccgcgg cctcgcggtt gcccataatg gcgtagtcgc tggcggccag
     6121 tacttgccgc tccatcaggc taaacggcgg tgggtaacgg cgggccagat tctgccaggt
     6181 gaagaacagc gttttggctc caacggcgcg cgcctggcgc atggccagcc aggtagccag
     6241 attgtatggc tcctcgtcga tgtggacgac gtcgggccgg aactggcgca gccggcgggg
     6301 cagggtggga tagtagtagg tgtggtagtg gccgttgaag cggatgggat cgaccagcag
     6361 ctcgtagccg tcggtgtagc agcgctccag cgccacccgg ccgtccgggt catcccagct
     6421 aggcggcaca agcgctgtca actcgacatc gggcatgcgc gcgatggctt ccagcttggt
     6481 ctgataggcc cccacgaggc aggccttgga gagcatgagg acacgcatag gctactccgc
     6541 ccgccgtggg gtggaggagg gcgatggcga tgacctgcgc atgtacttcg cggcgagatg
     6601 ggttcggtcg agttgacgtt gacgcaagga gctattttgc tatactgcgg ttgctttggc
     6661 aaccttgggg aagtcgcgtg cgcggtggtc tttcggccca tcccgttggg ggtgggcttt
     6721 gttgctcctg gcggtgctcg tcgccgcggc gctgcgcttc accaggctgg gcgcgtggcc
     6781 gcccggcccc taccgcgacg aagcctataa cggcctggac gcgctaggcg tcttgcgtgg
     6841 gcaactggcc cttttcttcc cggccaacaa cggccgcgaa ccgatcttca tctacctcgt
     6901 cgccctgtcc gttgccctgc tcggcccgac gacgctggcg ctgcggctgc cggcggctgt
     6961 ggccggcacg ctggcgacgc tgccagcggc gttgctgggg cgggcctggt ttgggcgcgt
     7021 ggccgggctg ctggccgcgt tcctgtgggc catcaccttc tggcccgtcc acctcggccg
     7081 catcggcttg cgggccgggc tgctcgcgcc catgctggcg acagccttct ggctgggcac
     7141 gcgcgcctac cgcgagcggc gggcggtgtt gtggctggcg gccgggctgg tctatggcct
     7201 cagcttctac acctatctgg cggcgcgctt cacgccgctc ttgttgctcc ttttcgccgc
     7261 gtacctggtg gccaccggcc gccgggcgcg cctgtgggac gcggggcggg cgctgtggtt
     7321 cgtcgccggg gcggccgtgg ccgtcgcccc gctggcgacc gtgctgctgc gcgacccggc
     7381 cctgctcttc ggccgcgccg ggcaggtgtc gatcctcagc ccggaggtca gcggcggcga
     7441 cccgctgggc gcgctgctgg gcaacaccgg ccgcgccctg ggtctgtacc tgtggcgcgg
     7501 cgatagtatc ctgcgccaca acgccttgct cagctacgac gtcgtgctga agagcgacaa
     7561 ccccgccgga cggccggtct tcgacctgct catggccggg ccgtttctcg tcgggctggg
     7621 ctggtgcctg tggcgctggc ggcggccggc ggcggccttt ctgctgctgt ggcaactggt
     7681 catgctcggc ccgacgctgt tggccgaaga cgcgccccac ttcctgcgcg ccgccggcat
     7741 cctgcccggc gcgatcttct tcccggccat tgggctggcc caattgtggg cctggccacg
     7801 gctgccggcg gtcgcgcgtc gcctggctgt cgttggcctg ctggccggca gcctggccct
     7861 gaccgtccgc gattacagca catacgcccg ccagccggac gtcgcttacc tgtgggagag
     7921 cgccgcggcc gagctggccc gcgccgcccg cgccgcgccg cagggcacga ccgtgctcat
     7981 tgaccggcgc ttcaccgagg gctggccgtc catccccttc ctgttgcacg gtcacgcctt
     8041 cacgcccttc gacccggcca agcctctgcc ggcgctggcc gggccgacga ccgtcttcgc
     8101 ctggccctat ggcccgctcg acttcctgcc tggggcggtc cgtccgccag cgcgggtggc
     8161 catcgaaccc ggcccgctgg cccgcggcga cctggagcca gagccgtacg cgctctacac
     8221 ccgctacgac atcctccccg gcccggccga agcgccaccg gcggcgaact tcgacgaccg
     8281 ctatggcctg agcgcggcgg cgacgctcga cgcgcccgac gcgctgacgg tggcgctgga
     8341 gtggacgccc ggcgcggccc actcgcccga cgcatcgccg ccgcaagtct tcatccacgt
     8401 cctcggccca gaggggatgc tggcccagca tgacggcact atcggcggag gtctgtggcc
     8461 ggcagcgaac tggcagccct tgctcaggat cgacgagcgg catacactcg cattgtcgcg
     8521 gccgttccag ccggcgcagg accgcatcga gatcggtctc tacgacccgg ccactggcca
     8581 gcggctgccg ttgctcggcg cgagcggcga tgtggtcgtc ctgacgccgg acggctgacg
     8641 gcaaggagcg atttggcatg aaagcaccgc gatggctgcc gatggggctg ctcgtgggcg
     8701 cgctgttggc cgccggggcc gcgtccgccg cccagaccga cccgacggcg atgcccatcc
     8761 ccacgccaac cattgagacg ccgacgccga cgtttgcgcc gccgacgccg tggatctcgc
     8821 ccacgccgga cgaaacaggc gcgatcacca tcatcgtcgc gccgggcgag tcgctgtggg
     8881 tcattgccgc gcgggccggg ttgtcgctgg gcgagctgct ggcgctcaac aacctgaccg
     8941 agggggatgt catcaacccc ggcgacgtgc tgctcgtcgc ccgcgtgacg ccgccggccg
     9001 cgcccaccag cggcagcccg acagtcacgc ccccgccgcc gacactgcgc cccaccgaaa
     9061 cgcgcgccga ggcgggcatc tgcctggagg cctacgacga tctcgaccgc gacggcatcc
     9121 acgacgaggg cgagccgttg cgggcgggcg tggccttcac catctacaac accgaggtcg
     9181 tcgtggccaa ctacattacc gacggcgtct ccgagcctca ttgcatcgcc gggctgctgc
     9241 cgggcgaata ccgcgtcacg cgctccatcg gccccggcga ggttctaacg acgggcggcg
     9301 actggacact gagcctgacg gccggcagcc agttgcggca agcatttggc agcgtcaccg
     9361 gcgcggccga cgtggccgcg gggccggccg ccggcgcgac cgcgctgccc acgcccgcca
     9421 acccgcccgc ggcaaccacg tcggccccgt ccacagcccc ggcggccacg cccgccggcc
     9481 agacggccgc ggccggccgg tttatccgcc tcgactggcg gttagccggc gtggtcatcc
     9541 tttttctggg cggcttgctc cttctggcgg ctgtgctaat cttgcttttc agacagccgc
     9601 gcgtcgttac gggcgggggg caacgccctg aaccgaccaa cgagcggcgt ttccacgacc
     9661 tcgatgacct ttagtggggc gcgtcgtcaa cagccgcgcc cggacatgat ggcatgaaga
     9721 agatagcccc cctccttgtc gtatccctct ttgtcgccct cctcggcgcg cgcgtcgccg
     9781 cggcccagca gggcaatctg ctgcaaaatc ccggcttcga gcaacccttc agcggcggcg
     9841 cagccggcaa ctggcagaag tggagccaat cgacgccccg gagcgacgag gagtgcctgg
     9901 tggcctatca cgtcctgccg aagtggaatg tcgagacagc ggccggcttc ctgcgcgagg
     9961 gagcggcgtc gcaatacgtc ggcaacaact gggacacctg gtcgggcggc gtctatcaga
    10021 ccgtcgccgc cacgccgggg acgacctacc gcttctcctt ctacggccgt ggtcgaggca
    10081 gcaacgagtc ttcgccggcg gcctcggaga cggcgctgca aatgaacatg cgcgccggca
    10141 ttgaccccaa cggcagtggc cagtggaacg acgccgatgt cgtctggggc gcggccggct
    10201 cgccccacga cacctggcag caattcaccg tctcggccac ggctacgggc aaccagatca
    10261 ccgtcttcac cagcgctgac tggggcgtga ccggcgtcaa ccagtgccgg cagttcctcg
    10321 acacgtgggt ggacgcggcc gagctagtgg cccagacgct gccaacggcc acgccccagc
    10381 cgacgcagcc cgcgccgacg cccgcgcccg tgcaaacgtc gtccgtgccc acggagccgg
    10441 ccgcggccac cgtggcggcg acactaacgg cggccacggc cacaaccgcg ccgacggccg
    10501 cgcccgcggg cacggccacc gtctgcgtca acgccttccg cgacgccaac ggcaacggcc
    10561 tgcgcgacgc cgacgaaggc tacgtcgccg gggtgacgtt gaccgtcgcc cagggcgcaa
    10621 cggtcatcgg gcaagcggtc tccaccggac aggcggagcc gctgtgcttc ggcaatctgg
    10681 ccgcggggac gttccaggtc ggccagacgc tgccgccgac attggagacg acgacgcagc
    10741 ccaacgccac ggtcgatgtc gctgccgggc agacgatcgc tctggagttc ggcagccggc
    10801 tgcggacgac gccgacggcc gagccggcca cgccaacggc cgaccccgct caggccgcgg
    10861 ccacggccac ggccgccgcg gtagccacgg gcgcgccgga gggcggctcg aactggttgg
    10921 tttacctggg gctgggggcg attctcgtcg gcgtggcgct attaggggtg ctgctcttca
    10981 cgctgctgcg gcgctagtcg gccgaggcgg gcaacagcgc gtttcatttg ttgtcccccc
    11041 tctatgatgc cgtatagagc cgttccacct tcatttcgcg ctgcctctgt cgggcctgcc
    11101 agagatcgta tcgtctcact tacctaagcg tgaaaaagaa ctcgaaccgc tgggcgaggg
    11161 cgaaggcctc atcggcgggc aagttctcgt aaaaggcact ggagtcatgc gtgttcagca
    11221 ggttatccaa agtcacaaga atcctgcctc aaggaactcg atgtttctct ttatcgccta
    11281 cagggctaca gcaacgtatt cactgaccga gtgcggagta ggaataggca ggccgtcctc
    11341 ttgcatgagc tccaaatgga ggacaatcgc ttcttggatt aaggtcaata cctcatcttt
    11401 ggtatccgca accgccacgc agccaggcaa atcgggcacg taggccccgt agctgtcttc
    11461 cccgcgttcg atgacaacca tgtattccat gctttttact ccttcagttg agcttgcttc
    11521 agaacactat tgagcgttcc tggtggcatg tctttgccag ggctgcccga tacagtcacg
    11581 atgccgggct tgcttagatg cctgaactgt cgatgacttc ctctaatccg cactaggtac
    11641 caaccgtcct cctcgatcag cttgatgacc tctcgaacct tcatgggtaa actccgatat
    11701 agcaatcggc ttcagcgaat acagcacctg cggcccgaat aataccacaa ctgacgatcc
    11761 ctctactact tgctatactc ccctcagagc gcaaacccgg tttctttcaa tactcagcaa
    11821 aggggatcac atggccctac acccactggc cgggcagccg gccccacgcg aacgacttgt
    11881 caatgtcccc aggctcgtct cagcctacta taccaaccac cccgacccgg ccgacccgct
    11941 gcaagcggtc agcttcggca cgtccggcca ccgcggcaca tcgacagggg gcacgttcaa
    12001 cgacgaccac atcgccgcca tcagtcaggc cgtggccgaa taccgcgccg ggcagggcat
    12061 cgctgggccg ctcttcgccg gcatggacac ccacgcgctg tcggagccgg ccctggccac
    12121 ggcgctggag gtcttcgccg ccaatggcgt ccatgtcgtc attcagcgcg gccttgacta
    12181 cacacccacg ccggtcatct cccacgccat cctgacgtgg aatagcgacc gcgcgcgccc
    12241 gcgggccgat ggcatcgtca tcacaccgtc gcacaacccg gccgaggacg gcggctacaa
    12301 gtataacccg cccgaaggcg gcccggcgga cacaacgacg accaggtgga ttcaggaacg
    12361 ggccaacgcc ctgctggccg atgggttgcg tggcgtgcga cgcattcccc tgcgccgggc
    12421 actggcggcc gacacgactg aggagcgcga ctacatcacc ccctacgtcg aagacctggc
    12481 caccgtggtc gatatggagg ccatcgccgc ggccaggctc cacatcggcg tcgatccaat
    12541 gggcggctcc ggcgtccact actgggaacc gattgccgcg cgctacgggc tgcacctgac
    12601 cgtcgtcaac ccctacgtcg atccgacctt tggcttcatg agcgtcgatc acgacggcaa
    12661 gatccgcatg gactgctcgt cgccctatgc catggccggt ctcatccgcc tgaaggacga
    12721 cttcgacatt gccgtcggca acgaccccga ctacgaccgc cacggcatcg tcacccgcag
    12781 cgccgggctg atgaacccca accacttcct ggcggtggcc atctggtatc tgttccagaa
    12841 ccgtccggcc tggcgcgccg acgcggctgt gggcaagaca ttggtgtcca gctcaatgat
    12901 cgaccgcgtg gccgcgcatc tgggccggcg gctggccgag gtgcccgtcg gcttcaagta
    12961 cttcgtcgct ggcttgctga gcggcgacta cggcttcggc ggcgaggaga gcgccggagc
    13021 ctcgttcctg cgccgcgacg gcagcgtatg gacgaccgac aaggacggct tcatcatgga
    13081 cctgctggcc gccgaaatcc tggcccgcac cggccgcgac cccggcgagc actacggcga
    13141 cctggccggg cgcttcggcg acccggtcta ccagcggacc gacacgcccg tgtccgcgac
    13201 gcgcaaagcc gtgctggcca acctgtcgcc agaactggtg cgcgccgata cgctggcggg
    13261 cgagccgatt gtcgccaagc tgacccgcgc ccccgccaac gacgccccca tcggcgggct
    13321 gaaagtcgtg accgccaacg gctggttcgc cgcccgtccc agcggcacgg agaacatcta
    13381 caaggtctac gcggagagct ttctagggcg cgagcactta cagcagattc aggcggaggc
    13441 cggcacgatc atcgacgacg ccttcgccgc ggcgggcgtg taaggtgagc gacaccttcg
    13501 ccgcccgcta tggcccgtgg gcgctggtca caggcgcgtc gtcgggcatc ggccgcgcct
    13561 ttgcccgccg tctggccggg cgcgggctaa acgtcgtcct ggccgcgcgg cgggcggagc
    13621 ggctagaggc gctggcgggc gagttggccg ccgactgcgg cgtactggcc tgccccgtcc
    13681 ctgttgatct ctgccgcgat gacttcctcg acatccttcg cgccgccact gacgggctgg
    13741 acatcggcct gctggtcaat agcgctggct atagcctgac cgggccattc ctcgacgccg
    13801 accccgacga gatgctgcgc ctgttcaacg tcaacgctcg cgcgccgctg ctgctgacgc
    13861 gcgctttcgg cccggccatg cgcgcgcggc ggcgcggcgg catcgtcatg gtctcgtcgg
    13921 tcgtcgcttt cgtgggcacg ccccagtgga gcctctacgc ggccaccaag gcgttcgacc
    13981 tgctgctggg cgaggggttg gccgccgagt tgcgcgccga cggcgtggac gtgctgacgc
    14041 tggcccccgg cacaacgcgc accgaatttc tcgacgtagc cggcctgagc ggccgcatga
    14101 gcctgtcggc cgagcgcgtc gtcgatgacg ccctgggcca tctggggcgg tcggatatcg
    14161 tcgtgcccgg ctggttctac cgcggcggca cgctggccat gcgcctgttg ccgcggtcgg
    14221 tcaaccgggc ggtgttcggc cgcttcctgg ccgccatgcg gacggaggaa tagcgccatc
    14281 gcccgccgag ccggcccctg gcgtgcatgg ctcttcccgg cggtcgtggt cgtcgtcgcc
    14341 gtggtggccg cctggccggc ggtgtcgcag ccggggctgc tgaacacacg cggcggcggc
    14401 gacagtccct tcctgctgca acgcctccac cagctaacaa cagcgctggc cggcggccat
    14461 ttccccgtcc gctggatgcc cgacgcggcc tatggcgacg gctatccctt ctacaattac
    14521 tatgcgcccc tctcgatcta tatcgccgcg gccttccgcg gtctgggctt cagctacgtg
    14581 cgcgccatcc atctggcgca actggccggc ttcatcgttg ccgggctggg gacgttctat
    14641 ttggggcggc gctggctgag gtcagactgg gcggggctgc tggcggccgt ggcctacacc
    14701 gtcgcgccct tccacatggt caatgtctac gtccgcggcg actcgctggc cgagttctgg
    14761 gcgatggcct ggtatccggt ggtgctgctg gccgccgatg gcctggtgac cgcccaacgg
    14821 ccggcccgcc gccgcgccgt ggccctcttc gccctggcct acgccgccct catcctcagc
    14881 cacaacatct ccgcgctcat cttctcgcct ttcctgctgg tctatttgtc tctgcgtttc
    14941 tggaatgcct ccc
//
LOCUS       DAOSMQ010000011        14785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_4087_length_14785_cov_4.567821, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000011 DAOSMQ010000000
VERSION     DAOSMQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14785
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_14785_cov_4.567821"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..149)
                     /locus_tag="PK829_00850"
     tRNA            complement(68..149)
                     /locus_tag="PK829_00850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:Leu,seq:tag)
     gene            254..1657
                     /locus_tag="PK829_00855"
     CDS             254..1657
                     /locus_tag="PK829_00855"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOU39791.1"
                     /translation="MMSTLLVKNALVLATMDDAQREIAGGGLFVRDNVIAAVGPTAEL
                     PDTADTVLDLRDHVVLPGLVNTHHHLYQSLTRVIAQDADLFTWLKTLYPIWANLTDEA
                     VYVSTITGLAELALSGCTTSSDHLYIYPNDCTLDSQIRAAADIGLRFHAARGSMSLGE
                     SQGGLPPDRVTEDEDAILRDSRRLIETYHDAQRHAMIRVVLAPCSPFSVTPDLMRESA
                     ALARATGVRLHTHLAETIEEEAFCLETFGARPVPYVESLGWTGDDVWHAHCVHMSAGE
                     IGLFGRTGTGVAHCPSSNMRLASGIAHVLDLRAAGAPVGLGVDGSASNDGGHLLGEAR
                     QAMLLQRVAPDRYLSEAPGGRGGFAGLAGAMSAREALAMGTRGGAAVLGRDDIGHLAP
                     GMSADFIALNLNRLDYAGAWHDPVAALIFCHAPRVDLSVINGRVIVQDGQLTTVDLEP
                     HLRRHNAIAREMVDGRG"
     gene            complement(1654..6078)
                     /locus_tag="PK829_00860"
     CDS             complement(1654..6078)
                     /locus_tag="PK829_00860"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU39792.1"
                     /translation="MNVKVKIVGLLLAVLVLTGLPAWVEAGSRLSVVIAAGAEIALDP
                     ADCQIVVDPSGSIQAAINAAANNTVICVHGGIYNEAITIPVTRTNLTLSAYPGEQPVI
                     DGNKRIPGGLPGARFRALVEIYGSGTVLDGFEVRYSSARGLEVNANNAIVRHTAVHDN
                     WAEGLTVRNAGENSRVTGVLIENNRVYSNLRKVRYIPVIYRGLRTGSGPTDWLFDPDE
                     AWDNPFWTGRDVDLPEGALNALAMTFNDDGQTARIYAGSVRTTREGHIGEEFSADGTP
                     IDYTGRDLLFFEPSTQKWTLYFKGATLGVPAAAVIDAFQIDGSIPPENLPCPQCAPIV
                     MSFDTPISLTVDGAPTSIGSSDLVRFQPTAVGPGDVITAGAFTMERTAASMSLPAGAN
                     IDALDRTPDNRQLMSFAGDITLGGQTFRNEDLIAYDPPDAWTLYFDGSAIPFNPFSDD
                     LTAVWLDRNGHIYISGDPIGGSGLTFVFAEDSIARGNVIYNNYGEGLVAGRYTSRLTL
                     EDNISYDNDHANVYLNDTADVLVQRNIIYCTDDREFWRKGHAVEYRPGTGLQIRDEDF
                     NPMPPPSTGQVIINNIVAGCSTNFGVDTQQAGGGLNNGVVANNVFINARADAPTAVDN
                     VVFSGGTSYANSRFVNNMIVQTVPGDLTLIQGTADFSTLTVANNLYSSAPTGWFPGEV
                     GRVVGAPVFVNGIPPLPTVASPPNPADYLVDYDSPAFDSGQTVAEVMADYFGHSRLDT
                     GLPDIGIYELPYQGEIIIIQQAIPASAAQSFDYTATFSPAVFRLSHGQQVATGVLTAG
                     TYRVTVGEVDGWTTTSSCDDGSTAETIALDPGETVTCTFVSTRASQLTVRNDLQPATD
                     TPLFDFDLDGTTFQLGHESRTFVVTPGEAHAVTAAVPDGWERTAATCDNGDAPAAVTL
                     AAGEQVNCTFSHRKLGRIVIEKQTLPDGVAQAFVFAAGYAGDGFSLSDGQQNQSDLLS
                     PGSYAVAETLPAGWAQSSATCDDGSTPGAIALDPGETVTCVFVNAQVGLALTKTPTPG
                     SVTAPGGDVSFAVRVSNTGAAALQLTALSDSVFGDVADAGNPALVATDCALPQSLAAG
                     SDYTCAFTGHIGGAAGQTHHNTLTATAGALAQATAEAAVAIVAPPPGHIVIVKQTDPP
                     NTPGQFNFSASYDADGFALGHGQSNDSGPLPSGATYSVGENVPAGWTLASATCSNGSP
                     PGQIALAANETVTCTFTNRRTTSGPAATFYVTTASAGTVGGVAYAVGDIVAYNGLTGV
                     WSLVFDGSDVGWKKAIGDFEFLPDGAVLLTSSAGQTVGSLKLQVHDIARFAPTSLGST
                     TAGTFTLYFDGSDVGLSTASERIDALARKPDGTLLISTAGRATVKNGSTSIVAQDEDL
                     LAFHPTQLGATTSGTWNLATDGFDGSLLTGMAAENVNSAWYDGATGNLYLTVLNAFNI
                     GSISGSQRTVLQVTPARAVSAYWSASAYGGIDGLAIAP"
     gene            complement(6250..7719)
                     /locus_tag="PK829_00865"
     CDS             complement(6250..7719)
                     /locus_tag="PK829_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU39793.1"
                     /translation="MKLVAMVALILLLSAGVVSADGETARSYAPAVYDTVACTVVVEP
                     GSSIQKAISPARSGAVICVRGGVYAEALKIAPANAGITLMAYPGEQPILDGENRLPVI
                     TNKNRFAPLIMVNARNVTVDGLEIRRSTMRGITVSQGDVILRNLIVHDNYEVGVIATG
                     PTAAPLRNVLVENSIVYNNLLKNANGAAGGSALTFIAVENGTARGNVIYHNYGEGLVA
                     GRWTRNMVLEGNASYDNRGANIYLVNTTDPIVRGNYVFCTDDPISWRGKGGMYRPGPG
                     LQVRDENFKAPQPPPSSGQVIVNNIVVGCGVNFGVATQIAGGGLNNAIVAHNTFVNAR
                     SVSGESANNIEIAGNAALNNTQFVNNLILQSVPGTITRIQVAMGTPNLSTFMVSNNLY
                     SRTPGNGWVAAESGRLVTDPQLVNPVLPLQAALPAPANFALTATSPAVDAGATSIGVS
                     QDFFSDERTGTVDIGADELGGSTQFNLLGDPASLTFSGE"
     gene            8058..8906
                     /locus_tag="PK829_00870"
     CDS             8058..8906
                     /locus_tag="PK829_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39794.1"
                     /translation="MRVTLAGYLTDHKGRVLLRQPGARSLEPVLCPLPVGQEPATTLA
                     RAFRAATGLFVLPVRLVGVYYTRDNELTLSYRGALRGGELVTPTGQPPAGFFDGQPPP
                     GGLSAAHARQLADAFHHDGGPPKTAHSPERLAARLGRGPSRARDEAEVGWAATARLLA
                     AAGGRVLWTRSAASEPWRLPARPVATGDAPWQTAAALQRALGLGHAVTPSLRLIIIAA
                     DRPELSFVFLATYGENELTHGRGEQLLVAPADSQANLDAADAALAAEVLAAPAGAVAR
                     LSAVRP"
     gene            complement(8920..11679)
                     /locus_tag="PK829_00875"
     CDS             complement(8920..11679)
                     /locus_tag="PK829_00875"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOU39795.1"
                     /translation="MTTWYTRAVDGVLQELAVDGARGLDDAEVARRREQYGPNELTER
                     GGKSPWKILWQQFSSTMVLILIAAAVVSGLLGKATETIAIAAIVVLFALLGFVQEYRA
                     EKAMAALKKLAVPLVRVIRGGERREVSARELVPGDIVLLEAGSAVPADLRLIEAANLR
                     IQEAVLTGESEPVEKAVAPLTSVALPLGDRINMAYMGTAVTYGRGSAVVVGTGMDTEL
                     GEIAALIQDVEDSATPLQRRLDSVGKLLALAGVVVAGLVLAIGLFLREDPRDMFLTAV
                     SVAVAVVPEGLPAVVTVTLALGAQRMLRRNALIRKLPAVETLGAVTVICSDKTGTLTE
                     NRMTVTVIDVAGHYLELTGTAPHHPAPALGGNGAPAETPIHLEEQPPAIGLTLAGGAL
                     CNDAALQPDEETNRYAVLGDPTEGALLVAALQAGLRREGLLAVAPRVAELPFDSERKR
                     MTTVHRLPSADQLPPALRGLVGAGLRPDDHVVAFTKGAVDGLLDISTAIWVGGEPQPL
                     DDHWRERLMRANEQMAGNGMRVLGVGVQWIDGPDEPLERDLTIIGLVGMIDPPRPEVK
                     AAVATARAAGIRPIMITGDHPLTARFIAYDLGITDNARVMTGLMLDALSDAELARVVD
                     EVSVYARVTPANKLKIVETLQGKGHVVAMTGDGVNDSPALKRADIGVAMGIAGTDVTK
                     EASEMVLLDDNFATIVAAVEEGRVIYDNIRRFVKFSIAGNVGKVAVMLLAPLLLGREA
                     AVALLPLQLLWLNLLTDGLLGLGLGVEPAERNTMRRPPRDPQAGIFSEGLGGHVLWVG
                     ALIGAVALLAGRLFFDAADLADRTWQTMIFTTLAFLQVGQALASRSGRESVFTLGLRS
                     NPTLLLLAVGVLALQLVVLYAPPLEQFFGIVPLSGRQLLVAAALGSIGLVAIEVEKAF
                     LRRRA"
     gene            11806..12615
                     /locus_tag="PK829_00880"
     CDS             11806..12615
                     /locus_tag="PK829_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HOU39796.1"
                     /translation="MVAPEPLDALVAAVLSSAKYRAVSPDLVRAVGARELAVRPNLKA
                     AIKATKNALHQAGGAFLDAPIDYGRALARLREAAGAGDAALREAARAVMALHASTRER
                     LPILDAFYAETLAGLPPPGRVLDVACGLNPLARPWMGLPATAELIAYDIYADQIAFLN
                     DVFALTGLPGRAEVRDVVGRPPDTPADLALVLKTLPCLEALDRRAPERLLDALPAPRL
                     LVSFPAQSLGGRRKGMAAHYEAGFRRLLDARGWPATRFTFATEIAFLVEKK"
     gene            12761..13591
                     /locus_tag="PK829_00885"
     CDS             12761..13591
                     /locus_tag="PK829_00885"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39797.1"
                     /translation="MTTDQEAVETVITAIKQSKKYRDTSEETIRQLAVEAVATHKKAR
                     PAEKAVRRRLHSIMAPYLGDPDYDAAGELLNQTFVNGDEAAVRAVCRDLMHTHLSTRE
                     RLPILDTFYRDIFAVTGPPGRLLDIACGLNPLAFPWMGLPRQGTDLLAYDIHEPRIDF
                     LNHYFILQGLPPLAFVRDVALQAPGDSGDVALFLKEMPRFERNYPGHGRALLAALDVR
                     WLVVSFPTISTHGGRNLTNRYREFFHQLIDGTGWPVTELLFDTELVFCAEKRSLVSGQ
                     "
     gene            13627..14367
                     /locus_tag="PK829_00890"
     CDS             13627..14367
                     /locus_tag="PK829_00890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOU39798.1"
                     /translation="MHRFFIPPDRRAGTIVHFEADQAHQMARVLRLRPGDRVLALDGT
                     GRQYEVALETVDATAAGRVVAESVAAGETGARLTLYQSLLKRDKFEWVLQKGTEIGVA
                     AFVPVITRRSLVRDAGVEPDKLARWRRIIQEAAEQSGRGALPALRPPVPFATAVGEAT
                     GYGRALIAWEGETERSLAAVLAEPLSRSTVALFIGPEGGFDPVEVEAAVAGGVLPITL
                     GPRVLRTETAALVGAALVLYELGEMGAA"
     gene            14364..>14785
                     /locus_tag="PK829_00895"
     CDS             14364..>14785
                     /locus_tag="PK829_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOU39799.1"
                     /translation="MNDDWRAHCGAIADELRAVAAFGLNFARPGSFDAERWEHVLAAA
                     ARLSALSGDGAADDLLARYHANHFDIGPVASGEAAVFHDGRLLLIRRSDDGLWALPGG
                     ITDPGETLARTAQRELWEEAGLAGRVVRLLGIFDSRRW"
BASE COUNT         2385 a         4956 c         4957 g         2487 t
ORIGIN      
        1 gtcagggtca cagggcttac ctcttgaaag aaaaagcgtt gctgccgcgc aacgctttgg
       61 gttgaaatgg ggatggaggg actcgaaccc acacgccttg cgacacatga ttctaagtca
      121 tgcgcgtctg ccaattccgc cacatccccg acagggcatt gtgatattat agctcaggca
      181 gccattgctt gccaaaagcc ataagacgca atccttcgcg cggccctgcc tgcgccaacc
      241 gacaaggagc ctcctgatga gcaccctact agttaagaat gccctcgttc tggccacgat
      301 ggacgacgcg cagcgcgaga tcgccggcgg cggcctgttc gtgcgcgaca acgtgattgc
      361 ggccgtcggc ccaacggccg agctgcccga cacggccgac acggttctcg atctgcgcga
      421 ccacgttgtc ttgcccggcc tggtcaacac ccaccaccac ctctatcaaa gcctgacgcg
      481 cgtcattgcc caggacgccg acctgttcac ctggctgaag acgctttatc ccatctgggc
      541 caacctgacc gacgaggccg tctacgtcag caccatcacc gggctggccg agctggccct
      601 gtctggctgc acgaccagta gcgaccacct ctacatctat cccaacgact gcacgctgga
      661 cagccagatt cgcgccgcgg ccgacatcgg tctgcgcttc cacgccgccc gcggctccat
      721 gtcgttgggc gaaagccagg gcggtctgcc ccccgatcgc gtcaccgagg acgaagatgc
      781 catcctgcgc gacagccgcc ggctgatcga gacctaccac gatgcccagc gccacgccat
      841 gattcgcgtc gtgctggctc cgtgctcgcc cttctccgtc acgcccgacc tgatgcgtga
      901 gtcggccgcg ttggcccgcg ccactggcgt gcggctgcac acccacctgg ccgagaccat
      961 cgaggaggag gcgttttgcc tggagacgtt tggcgcgcgg ccggtgccct acgtcgaatc
     1021 gctgggctgg acgggcgacg acgtctggca tgcccactgt gtccacatga gcgcggggga
     1081 gatcggcctg ttcggccgca cgggtacggg cgtggcccac tgccccagca gcaacatgcg
     1141 cctggccagc ggcatcgccc acgtgctcga tctacgcgcc gccggcgcgc cggtgggcct
     1201 gggcgtagat ggctcggcca gcaacgacgg gggccacctg ctgggcgagg cgcgccaggc
     1261 aatgctcttg cagcgcgtgg ccccagaccg ctacctgagc gaagcgccgg gcgggcgggg
     1321 cggctttgcc gggctggccg gggccatgtc ggctcgcgag gcgttggcga tgggcacgcg
     1381 cggcggcgcg gccgtgctgg ggcgcgacga catcggccat ctggcccccg gcatgagcgc
     1441 cgacttcatc gccctgaacc tcaaccggct cgactacgcc ggggcgtggc acgacccggt
     1501 ggccgcgctc atcttctgcc acgcgccccg cgtcgacctg tcggtcatca acggccgcgt
     1561 tatcgtccag gacggccagc tgacgaccgt cgatctggaa ccgcacctgc gccgccacaa
     1621 cgccatcgcc cgcgagatgg ttgacgggcg cggctagggc gcaatggcca ggccgtcaat
     1681 gccgccgtag gcgctggctg accagtaggc ggagacggcc cgcgccggtg tcacctggag
     1741 caccgtccgc tggctgccgc tgatgctgcc gatgttgaag gcgttgagga cggtcaggta
     1801 gaggttgccc gtcgccccgt cataccaggc gctgttgacg ttctccgcgg ccatgccggt
     1861 cagcagcgac ccatcgaagc cgtcggtggc caggttccac gtgccgctcg tggtcgcgcc
     1921 cagttgcgtc gggtggaagg ccaacaggtc ttcgtcttgc gccacgatgg acgtgctacc
     1981 gttcttgacg gtggcccggc cggcggtgct gatgagcagc gtgccgtcgg gcttgcgcgc
     2041 cagggcgtcg atgcgctccg aggccgtgct caggccaacg tcggagccgt cgaagtagag
     2101 cgtgaacgtg ccggcggtcg tgctgcccag cgaggtaggc gcgaagcggg cgatgtcatg
     2161 cacttgcaac ttcaggctgc ccacggtctg cccggcgctg ctggtcagca gtaccgcgcc
     2221 gtcgggcagg aactcgaaat cgccgatggc tttcttccag ccgacgtcgg agccgtcgaa
     2281 gaccagcgac cacacgccgg tcaggccgtt ataggcgacg atgtcgccga cggcataggc
     2341 cacgccgccc accgtacctg cgctggccgt tgtcacgtag aaggtcgccg ccggcccgga
     2401 ggtagtgcgc cggttggtga aggtacacgt caccgtctcg ttggcggcca gggcgatttg
     2461 accgggcggg gagccattgc tgcacgtggc gctggccagc gtccagccgg cgggcacgtt
     2521 ctcgccgacg ctgtaggttg cgccggaggg cagcgggcca gaatcgttgc tctgcccatg
     2581 tcccagggcg aagccatcgg cgtcgtagct ggccgagaag ttgaactggc cgggcgtgtt
     2641 gggcgggtcg gtctgcttga cgatgacgat gtgccccggc gggggagcga cgatggccac
     2701 agcggcctcg gccgtagcct gggccagcgc gccggccgtg gccgtcagcg tgttgtggtg
     2761 ggtctggccg gccgcgccgc cgatgtggcc agtgaaggcg caggtgtaat cgctgcccgc
     2821 ggccaggctc tggggcaggg cgcaatcggt ggccaccagc gccgggttgc cggcgtcggc
     2881 cacgtcgccg aagaccgagt cgctcagcgc cgtcagttgc agcgccgccg cgccggtgtt
     2941 gctgacgcgc accgcgaagc tcacgtcgcc gccgggcgcg gtgacactgc cgggcgtcgg
     3001 cgtcttggtc agagccaacc ccacttgcgc gttgacgaaa acgcacgtga ccgtctcgcc
     3061 ggggtcgagg gcgatcgcgc cgggggtgga gccgtcatca caggtggcgc tgctctgcgc
     3121 ccagccggcc ggcagcgttt cggcgacggc gtagctgccc ggcgacagga ggtcagactg
     3181 gttctgctgg ccgtcgctca ggctgaagcc gtcgccagcg tagccggcgg caaagacaaa
     3241 ggcctgcgcc acgccgtcgg gcagcgtctg cttctcgatg acgatgcggc caagtttgcg
     3301 gtggctaaag gtgcaattga cctgttcgcc cgcggccagc gttacggccg cgggcgcgtc
     3361 gccgttgtcg cacgtggctg cggtgcgctc ccagccgtcg ggcacggcgg ccgtgacggc
     3421 gtgggcctcg cccggcgtca ccacaaaggt acggctctca tggcccagtt ggaacgtcgt
     3481 gccatccaga tcgaaatcaa acagtggcgt gtccgtcgcc ggctgcaagt cgttgcgcac
     3541 cgtcagctgc gacgcgcgcg tgctgacgaa ggtgcaggtc accgtttcgc cggggtcgag
     3601 agcgatggtc tcggccgtcg agccgtcgtc acagctcgat gtcgtcgtcc agccgtccac
     3661 ttcacccacc gtaacgcgat aggtgccagc cgttagcacg ccggtggcga cctgctggcc
     3721 gtgggacaac cggaagacgg ccggcgagaa agtcgccgta tagtcgaacg actgggccgc
     3781 gctggcggga atggcttgct ggatgattat gatctcgccc tggtagggca gttcgtagat
     3841 gccgatgtcg ggcaggccag tgtcgaggcg actgtggcca aagtagtcgg ccatgacctc
     3901 ggccaccgtc tggccgctgt cgaaggccgg cgagtcgtaa tcgacgaggt aatcggccgg
     3961 gttgggcggg ctggcgacgg tgggcagcgg tgggatgccg ttgacaaaga ccggcgcgcc
     4021 gacgacgcga cccacctcgc cggggaacca gcctgtcggg gccgacgagt acagattgtt
     4081 ggccacggtc agggtcgaga agtcggccgt gccctggatg agcgtcagat cgccggggac
     4141 ggtctgaacg atcatgttgt tgacaaagcg cgagttggcg taggacgtgc cgccgctaaa
     4201 aaccacgttg tcgacggccg tgggcgcgtc ggcgcgggcg ttgataaaga cgttgttggc
     4261 cacgacgccg ttgttcagcc caccgccggc ctgctgtgta tctacgccga agtttgtgct
     4321 gcaaccggcg acgatgttgt tgatgatgac ctggccggtg ctgggcggcg gcatagggtt
     4381 gaaatcctcg tcgcggattt gcaggccggt gccgggccgg tattcaacgg cgtgcccctt
     4441 gcgccaaaac tcgcggtcat cggtacagta aatgatattg cgttgcacca acacgtcggc
     4501 cgtgtcgttc aggtagacgt tggcgtggtc attgtcatag ctgatgttgt cttccagggt
     4561 gaggcggctg gtatagcggc cagccaccag cccttcgcca tagttgttat agataacatt
     4621 gccgcgggcg atgctgtctt cggcaaagac gaaggtcaga ccgctgccgc cgatggggtc
     4681 gccactgatg tagatgtggc cgttgcggtc gagccaaacg gcggtcaggt catcagaaaa
     4741 agggttgaag gggatggcgc tgccgtcgaa gtagagcgtc caggcatcgg gcgggtcata
     4801 ggcgatgagg tcttcgttgc ggaaggtctg gccgcccagg gtaatgtcgc cggcaaagct
     4861 catcagttgg cgattgtcgg gtgtgcggtc gagggcgtcg atgttggccc cggccggcaa
     4921 gctcatgctc gccgcggtcc gctccatcgt gaacgctccg gcggtgatga catcgcccgg
     4981 gccgacggct gtgggctgga agcgtaccag atcgctggag ccgattgacg ttggcgcgcc
     5041 gtcgacggtc aggctgatcg gcgtgtcgaa gctcatgacg atgggcgcgc actgtggaca
     5101 gggcaggttc tctggcggaa tgctaccgtc aatctggaaa gcgtcgatga ccgcggccgc
     5161 gggcacgccc agcgtcgcgc ccttgaagta gagcgtccac ttctgcgtcg atggctcaaa
     5221 gaacagcagg tcgcgccccg tgtagtcgat gggcgtgcca tcggcgctga actcctcgcc
     5281 gatgtggccc tcgcgggtcg tccgcaccga gccggcatag atacgcgccg tctggccgtc
     5341 gtcgttgaac gtcatcgcga gcgcgttcag agcgccctcc ggcaaatcga cgtccctgcc
     5401 cgtccagaat gggttgtccc atgcctcgtc gggatcgaac agccagtcgg tcgggccgct
     5461 gccggtgcgc aggccgcggt agatgaccgg gatgtagcgc accttgcgca ggttgctgta
     5521 gacgcggttg ttctcgatca ggacgcccgt cacccggctg ttctcgccag cgttgcggac
     5581 agtcaggccc tcggcccagt tgtcgtgaac ggcggtgtgg cgcacgatag cattgttggc
     5641 attgacctct agcccccgcg cgctggagta gcgcacctcg aagccgtcaa gcaccgtgcc
     5701 agagccgtag atctccacca gcgcccggaa gcgcgccccg ggcaggccac cggggatacg
     5761 cttgttgcca tcgatcacgg gttgctcccc cggataggcg ctcagcgtca ggtttgtcct
     5821 ggtgacggga atggtgatgg cttcattgta gatgccgccg tggacacaaa taaccgtgtt
     5881 gttggccgcg gcgttgatgg cggcctggat ggagccactg ggatcgacga cgatctggca
     5941 gtcggccggg tcgagagcaa tctccgcgcc ggcggcgatc acgacactca gccgactccc
     6001 cgcctctacc cacgcgggca agccggtgag aaccaatacg gccagcagca aaccaacgat
     6061 ttttaccttt acgttcatgg caagcacacc tgtacaaaga attgggccat tgtaatttta
     6121 agggaactcg cttacgaagc ttattactgc cagcgccggc cccggaaaac gcgcgacggg
     6181 tcaggcggct gcctgacccg tcgcaagaca aactgttcac tctgcggtgt gcgttgccgt
     6241 gtctgccggc tactcgccgc tgaaagtcag agaggccggg tcacctagca ggttgaattg
     6301 tgtgctgccg cccagctcgt ccgcgccgat atcgactgtg ccggtgcgct catcgctgaa
     6361 gaagtcctgg ctgacgccga tgctggtcgc gccagcatcc accgccggag aggtcgccgt
     6421 cagggcgaag ttcgccggcg ccggcaaagc ggcctgtaac ggcagaaccg ggttaaccaa
     6481 ttgcggatcg gtgacaaggc gacccgactc ggcggcaacc cagccgttgc ccggcgtccg
     6541 gctatagagg ttattgctga ccatgaaggt ggataggttc ggtgtgccca tcgccacctg
     6601 gatgcgcgtg atcgtgcccg gcaccgattg caggatcagg ttgttgacaa attgcgtgtt
     6661 attcagcgcc gcattgccgg cgatttcgat gttgttggcc gattcgccgc tgacgctgcg
     6721 cgcgttcacg aacgtgttgt gggccacgat ggcgttgttc aggccgccac cagcgatctg
     6781 tgtggcgaca ccgaagttga cgccacaacc cacaacgatg ttgttgacga taacctgacc
     6841 cgaactgggt gggggctgag gcgctttgaa gttctcatcg cgcacctgga ggccggggcc
     6901 ggggcgatac ataccgccct tgccacgcca cgagatggga tcgtcggtgc aaaagacgta
     6961 gttgccgcgg acgatcggat cggtcgtgtt caccagatag atgttagcgc cgcggttgtc
     7021 gtagctggca ttgccctcca ggaccatatt gcgcgtccag cggccggcca ccaggccttc
     7081 gccgtagttg tggtagatca cattgccgcg ggccgtgcca ttctcgaccg cgatgaacgt
     7141 gagggcgctg ccgccggccg cgccattggc gttcttcaac agattgttgt agacgatgct
     7201 gttctctacc agcacattgc gcaggggggc cgccgtaggc ccggtcgcta tgacgccaac
     7261 ttcgtagttg tcatgaacga tcaagttgcg gaggataacg tccccctggc taaccgtgat
     7321 accgcgcatg gtcgagcggc ggatttccag accgtcaaca gtgacgttcc tcgcgttgac
     7381 catgatcagc ggggcaaacc gattcttgtt tgtgatcacc ggcagccggt tctcgccgtc
     7441 cagaataggc tgctcgccgg gataagccat gagggtgatg ccagcattgg ctggagcgat
     7501 tttgagcgcc tcagcataga cgccgccgcg gacgcagatg accgcgccgc tgcgggcggg
     7561 gctaattgcc ttctgaatgc tgctccccgg ctcaaccaca actgtgcagg cgactgtgtc
     7621 atagaccgcc ggcgcatacg agcgcgctgt ctcaccatcg gccgacacga cgccagccga
     7681 tagaagcaaa atcagcgcga ccatggcaac cagtttcata ctctttgctc tcatctcctg
     7741 cattctcccc gattgcggcg acgcgtgagg acgctaggga gaatcgccgc ctagcgcagt
     7801 acaggtatac tgcctgacca aatatgcgat ttggtagcca acgagtgccg atgtgttgaa
     7861 caacaccggt gataaaatag agtagccgac gcccgttgag agcggtggtt ggtgatggtg
     7921 gaggcgtgga cagttcccga tactgtcctg actgctaccc tatcgttcca gatagcgtgg
     7981 cgctttgctg gtttacggca acacacaaat tactcagaaa agggccacgc aacccactgt
     8041 atcggagaag tattacaatg cgcgttacac ttgccggtta cctgaccgat cataaaggcc
     8101 gtgtcctcct gcggcagccg ggcgcgcgct ctctggaacc tgtcctttgc ccgttgccgg
     8161 tggggcaaga accggcaacg acgctggcga gggcctttcg cgcggccact gggctttttg
     8221 tcctaccggt ccggctggtg ggggtgtact acacgcgtga taacgagttg accctcagct
     8281 accgcggcgc gttgcgcggc ggcgaactgg tcacgcccac ggggcagccg ccggccggct
     8341 tcttcgacgg ccaaccgccg cccggcggcc ttagcgccgc ccacgcgcgc caactcgctg
     8401 acgccttcca ccacgacggc ggcccgccca agacggccca ttcgccggag cgcctggcgg
     8461 cgcggctggg gcgcggcccc agccgcgcgc gggatgaggc ggaggtcggg tgggcagcga
     8521 ccgcgcggct gctcgccgcc gccgggggtc gcgtcttgtg gacgcggtcg gccgccagtg
     8581 agccgtggcg cttaccggcg cggccagtgg ccaccggcga cgcgccctgg cagacggccg
     8641 ccgcgctcca gcgcgccctc ggcctgggcc atgccgtcac gccatcgcta cgcctcatca
     8701 tcatagcggc cgacagacct gagttgtctt ttgtcttcct ggccacctat ggggagaatg
     8761 agttaacaca tgggcgcggc gagcagctgc tcgtcgcgcc cgcggactcg caagccaacc
     8821 tcgatgcggc cgacgcggct ctggccgctg aagtgctggc cgcgccggcc ggcgctgtgg
     8881 cccggctgag cgcggtgcga ccctagccgg cggcgcgcct tatgcccgcc gccgcaagaa
     8941 cgctttctcc acctcgatgg ccacaaggcc aatgctgccc agcgccgccg ccaccagcag
     9001 ttgccgcccg ctcagaggca caatgccgaa gaactgctcc agcggcggag catagagcac
     9061 cacgagttgc agcgccagca cgcccacggc caggagcagc agcgtcgggt tcgagcgcag
     9121 gcccagcgtg aaaactgact cccggccgga gcgcgaggcc agcgcctgac cgacctgcaa
     9181 gaaggccagc gttgtgaaga tcatcgtctg ccacgtccgg tctgccaggt cggcagcgtc
     9241 gaaaaagagc ctgccggcca gcagcgccac cgcgccgatg agcgcgccca cccagaggac
     9301 gtggccaccc agcccctcgc tgaagatgcc cgcctggggg tcgcgcggcg gccggcgcat
     9361 cgtgttgcgc tcggccggct ccacgcccag acccagcccc agcaggccgt cggtcagcag
     9421 gttcagccac agcagttgca gcggcaagag ggccacggcc gcctcgcgcc ccagcagcag
     9481 cggggccagc agcatcaccg ccaccttgcc gacgttgccg gcaatggaga acttcacaaa
     9541 gcgccggata ttgtcgtaga tgacgcgccc ttcttcgacc gcggcgacga tggtggcgaa
     9601 gttatcgtcc agcaacacca tctccgacgc ctctttggtc acgtcggtgc cggcgatgcc
     9661 catggccacg ccgatatcgg cccgcttcag cgccggcgag tcgttgacgc catcgccggt
     9721 catggctacg acgtggccct tgccttgcag cgtctcgacg atcttcagct tgttggccgg
     9781 cgtcacgcgg gcgtagaccg aaacctcatc gaccacccgc gccagttctg cgtccgacag
     9841 cgcgtcgagc atcaggccgg tcatcacgcg ggcgttgtcg gtgatgccca ggtcataggc
     9901 gataaagcgg gcggtcagag gatggtcgcc ggtgatcatg atcgggcgaa tgccggcggc
     9961 ccgggctgtg gccacggctg ccttgacctc cggccggggt gggtcgatca tgcccaccag
    10021 gccgatgatg gtcaggtcgc gctccagcgg ctcgtcgggg ccgtcgatcc actgcacgcc
    10081 aacacccagc acgcgcatgc cattgccggc catctgctcg ttggcgcgca tcagccgctc
    10141 gcgccagtgg tcgtccaacg gctgtggttc gccgccgacc cagatagccg tgctgatgtc
    10201 gagcagcccg tccaccgcgc ccttagtgaa ggcaacaacg tgatcgtctg gccgcaggcc
    10261 cgcgcccacc agtccccgca gcgccggcgg caactggtcg gcgctgggca ggcggtggac
    10321 ggtggtcatg cgcttccgct ccgagtcgaa aggcagttcc gccacacgtg gggccacggc
    10381 cagcaagccc tctcggcgga ggcccgcctg cagcgccgcc accagcagcg ccccctcggt
    10441 cgggtcgccc agcacagcgt agcgattggt ctcctcgtcg ggttgcaacg cggcgtcgtt
    10501 gcacagcgcg ccgccggcca gcgtcaggcc gatagcgggc ggctgttcct ccaggtggat
    10561 gggcgtctcg gccggcgcgc cgttgccgcc cagggcgggc gcggggtggt ggggggccgt
    10621 gcccgtcagt tccaggtagt ggccggccac gtcgatgacc gtgacggtca tgcggttctc
    10681 ggtcagcgtg ccggtcttgt cggaacaaat gaccgtgacc gcgccgagag tctcgacggc
    10741 cggcagcttg cggatgaggg cgttgcggcg caacatgcgc tgcgcgccca gcgccagcgt
    10801 gacggtgacg acggccggca atccctccgg cacgacggcc acggccacgc tgacggcggt
    10861 caggaacata tcgcgcggat cttcgcgcag gaagaggccg atggccagca ccaggccggc
    10921 cacgacgacg cccgccaggg ccagcagctt gccgacgctg tcgaggcgac gctgcaacgg
    10981 cgtggcgctg tcctccacgt cctggatgag cgcggcgatc tcgcccagct cggtgtccat
    11041 gcccgtgccg acgacgacgg ccgacccgcg gccatacgtc accgccgtgc ccatgtaggc
    11101 catattgatg cggtcgccca gtggcagggc caccgaagtc agcggggcga ctgccttctc
    11161 caccggctcc gattcaccgg tcagcaccgc ctcctgaatg cgcaggttgg cggcctcgat
    11221 caggcgcagg tcggcgggca cagccgagcc ggcctccagc aggacgatgt cgcccggcac
    11281 cagctcgcga gcggagactt cgcgccgctc gccgccgcgg atgacgcgca cgagcggcac
    11341 ggccagcttc ttcagcgcgg ccatggcctt ctcggcgcgg tattcctgca caaagcccag
    11401 cagggcaaag agcacgacga tggcggcgat agcgatcgtc tccgtcgcct tacccagcag
    11461 gccggagacg accgccgcgg cgatgaggat gagcaccatc gtgctgctga attgctgcca
    11521 gaggattttc cagggcgact tgccgccacg ttcggtcagt tcgttggggc catattgctc
    11581 gcgccggcgg gccacttcgg catcgtccag gccgcgcgcg ccgtcaacgg ccagttcctg
    11641 cagaacgcca tcgacggcgc gtgtatacca cgttgtcatg ttgggtttct ctcctgtgtc
    11701 ctgcgcgcaa caaacgcgca tggggcaggg attatgtttt atagacaatt gttagacaaa
    11761 taatgttaat ctgcctctag gcaactttgt ggtacgcttc gcggtatggt tgcgcccgaa
    11821 cccctcgatg ccctggtagc cgccgtcctg tcgtcggcca agtaccgcgc cgtcagcccc
    11881 gacctggtgc gggccgtcgg cgcgcgcgag ctggccgtcc gacccaacct gaaggcggct
    11941 atcaaggcga ccaagaacgc gctgcaccag gccggcgggg cgttcctcga cgcgcccatc
    12001 gactatggcc gcgccctggc ccggctgcgc gaggccgctg gcgcgggcga cgccgccctc
    12061 cgcgaggcgg cccgcgccgt catggccctg cacgcctcga cgcgcgaacg gctgcccatc
    12121 ctcgacgcct tctacgccga gacgctggcc ggcctgcccc cacctggccg cgtgctcgac
    12181 gtcgcctgtg gcctgaaccc tctggcccgg ccgtggatgg gcttgccggc gacggcagaa
    12241 ctgatcgcct acgacatcta tgccgaccag atcgccttcc tcaacgacgt tttcgccctg
    12301 accgggctgc ctggccgggc tgaggtgcgc gacgtggttg gccgcccgcc cgacacgccg
    12361 gccgacctgg ctctcgtcct caagacgctg ccctgcctgg aggcgctcga ccggcgcgcg
    12421 ccggagcgac tgctcgacgc gctgcccgcg ccgcggctgc tggtgtcctt cccggcgcag
    12481 agcctcggcg gccggcgcaa gggcatggcc gcccactacg aggccggctt ccgccgcctg
    12541 ctcgacgccc gcggctggcc ggccacccgc ttcacctttg ccaccgagat cgcttttctg
    12601 gtcgagaaga agtgagacat ggcacgcaga tcaaactgag tggcgcgatt ctcccgaatc
    12661 gcgcgctgaa gggaaatcgc gagtcggaga atcgcgccac aagtacaggt cctaccgcca
    12721 actgggctac aataggggca aagcgcgaag gggcaagcct atgacaaccg accaggaagc
    12781 cgttgagacg gtcatcacgg ccatcaaaca atcgaaaaag taccgcgaca ccagcgaaga
    12841 gaccattcgc caactggccg tggaggccgt ggccacgcac aagaaggccc gcccggccga
    12901 gaaagccgtc cgccgccgcc tgcacagcat catggccccc tacctgggcg atcccgacta
    12961 cgacgccgcg ggggaattgc ttaaccagac cttcgtcaat ggcgacgagg cggccgtccg
    13021 cgccgtctgc cgcgacctga tgcacaccca tttgtccacc cgcgagcgcc tgcctatcct
    13081 ggacacgttc taccgcgaca tcttcgccgt caccggtccg cccggccgcc tgctcgacat
    13141 tgcctgcggc ctgaacccgc tggccttccc gtggatgggc ctgccgcgcc agggcaccga
    13201 cttgctggcc tacgacatcc acgagccgcg tatcgacttc ctcaaccact acttcatctt
    13261 gcaggggctg ccaccgctgg cctttgtgcg cgacgtggcc ctgcaagcgc ccggtgacag
    13321 tggcgacgta gccctgttcc tgaaagagat gccgcgtttc gagcgtaact accccggcca
    13381 tggccgcgcc ctgctggcgg cgttggatgt gcgctggctc gtggtctcct tcccgaccat
    13441 cagcacccac ggcggacgca acctgaccaa ccgctaccgc gaattcttcc accagctcat
    13501 cgacggcacg ggctggccgg tgacggaact gctgttcgac accgaacttg ttttctgtgc
    13561 cgagaaaagg tcgctggtca gtggccagtg atcactggcc agtcctccta accacttccc
    13621 catcccatgc accgcttctt catccccccc gaccgtcggg ccggcacgat cgtccacttt
    13681 gaggccgacc aggcccacca gatggctcgc gtcttgcggc tgcggccggg cgaccgcgtt
    13741 ctggcgctcg acggcacagg ccggcagtac gaagtcgccc tggagacagt cgatgccacc
    13801 gccgccgggc gcgtcgtggc tgagtcggtc gctgccggcg agacgggcgc gcgcctgacg
    13861 ctctaccaga gcctgttgaa gcgcgataag ttcgagtggg tgctgcaaaa gggcacggag
    13921 atcggcgtgg cggcatttgt gccggtcatc acccgccgct cgctggtgcg cgatgccggc
    13981 gtggagccag acaagctcgc ccgctggcgg cgcatcatcc aggaagcggc tgagcagtcg
    14041 ggccggggcg cgctgccagc cctgcgtccg cccgtgccct tcgccacggc cgtgggcgag
    14101 gccactggct atggccgcgc cctgatcgcc tgggagggcg agacggagcg cagcctggcc
    14161 gcggtcctgg ccgaaccgct ttctcgatcc acggtcgccc ttttcatcgg cccggagggt
    14221 ggcttcgacc cagtggaggt cgaggcggcg gttgccggcg gcgtgttgcc aataaccctg
    14281 ggcccgcgcg tgctgcgcac agagacggcc gccctggtcg gcgcggcgct ggtgctctat
    14341 gagttgggcg agatgggcgc ggcgtgaacg acgactggcg cgcccactgc ggagccatcg
    14401 ccgacgagtt gcgggccgtg gccgcctttg gactgaactt cgcccggccg gggtcgttcg
    14461 acgccgagcg ctgggagcat gtgctggccg ccgccgcccg cctgtcagcg ctcagcggcg
    14521 acggcgcggc cgatgacctg ctggctcgct accacgccaa ccacttcgac atcggcccgg
    14581 tggccagcgg cgaggcggcc gtcttccacg acggccggct gctgctcatc cgccgcagcg
    14641 acgacggcct gtgggcgctg cccggcggca tcaccgaccc cggcgagacg ctggcccgca
    14701 cggcgcaacg cgaactgtgg gaagaagccg gcctggccgg gcgcgtcgtc cggctgctgg
    14761 gcatcttcga ctcgcgccgc tggca
//
LOCUS       DAOSMQ010000012        13797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_4464_length_13797_cov_4.823170, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000012 DAOSMQ010000000
VERSION     DAOSMQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4464_length_13797_cov_4.823170"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="PK829_00900"
     CDS             complement(<1..2323)
                     /locus_tag="PK829_00900"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HOU39800.1"
                     /translation="MKRQATYLSIASVVAAFLLALIVTTPTRAAAPLITPDQKFSIVE
                     NSPNGSSTTPSVLDAFDPEGEPVTFDILGGDGANAFDINSTTGVITVEDSTLLDYEVK
                     KSFQLNIRVNDTLAPVESAETTITINLIDASDQPPVMGDQTFSVAENSPAGTTVGTLV
                     YTDGDTNDSHTFTITGGSGNGLFNIDSLGKITVAPGAQLNYEAVNSYNMTVEIKDEGG
                     LTDTALITINVTDLNENPVVTPATFTLAEDAANGAVVGKVDADDPEKGPLTYSLSGTT
                     IFAIHPNSGQITVANSALLDFETNPTITFSVQAMDEKGKSGSATITVNLTPVNDPPKT
                     TGIPDQVVNEGGAPKVINLWQYFSDDEDPDAALTFVVQNNSNPSLVTAQVNNDAGTLT
                     LTFAASGAGVANITVRAFDSQDAHTDDPFVVDINDAPTGPATQNVTVNEDALTSQINL
                     YDVFDDAESADADLTYAIISVSNPALFDPAPTITKPNLTLDYAPDAAGKSDVKVRATD
                     SGGLWAETTFKVTVNAVNDNPTTSGIANVTVNEDAPDTVINLAPAFADKEDADNELDY
                     SVTNNTNENLFQSVTVDDAALTLTLDYKANAFGTASLTVQARDTDNGTVQTTFQVTVG
                     ATNDAPTLTDIALSTDEDTAVNFTANTFTSHFNDADGDSLVRVRIDSLPSDGTLKLNN
                     SPITSGVEIPVGELGNLTFVPAPNWNEGSTSFEWNASDGSNYAAASAQVTITVQSQND
                     KPTVNDFQKTGDEGVNILFAAADFIGAFNDVDGD"
     gene            complement(2629..4479)
                     /locus_tag="PK829_00905"
     CDS             complement(2629..4479)
                     /locus_tag="PK829_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOU39801.1"
                     /translation="MVARDKEDLSGQQIGNYEIESHIAARKASDLFLARDVKLERLVF
                     LEVLRTTTEEDENLAARFRRRMETVSQLKDPSIAVVSDLDITDDGFPYAVIDYFPGVT
                     LADKLADLARAQDKLPVIAALELAKHLAQALGVAHAAGLIHHDLRPENVMLREDGTPV
                     LIDLGLPLVLDPAAPGVNGSESEELDYASPEELEGKGITRRSNIYSFGVILYELLSGH
                     RPKLPVLPFDIFPQANMPKEEPLEEARPGLAGETYRLVRNCLWRQEWSRFETADELIT
                     AIETAIFAEQELPKAATWTPKPNRSFLVIIPVALLVLGLLGFLILRNARSGQAEAEDT
                     PVAETTPNDVAGVATANPDVTPSPTTTATIEFSTESAGEVAIPALSPGPGRAFTIADT
                     IIFDWYWPALPEPGQHFALYLIDGDQEHKLGTLTEPNNGISYRLTVDGADIPVTGEDL
                     GWQVRLENVNGGGVEVASDVIPIIIRELTPTPSPTGTPAPQTPTATPTLAEACVVSPP
                     PGWVLYTVRQGDSLSNLAERANIPVESLLQVNCLPNDLLSVGQQVWVPLAAVPRTPTP
                     TSPTATSAPPPQQPTNTPQPEVPTNTPQPEQPTDTPPPELPTKTPPGQQP"
     gene            complement(4661..5239)
                     /locus_tag="PK829_00910"
     CDS             complement(4661..5239)
                     /locus_tag="PK829_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39802.1"
                     /translation="MSTMGSGGKAARLATPLLLLLAIGGWYWLSQSAVARPTAVTVVT
                     PMRASATAASPMAASTAPPQGLIGQATPWGAAGLEPTATATVAVVAPGAIALYGPPAG
                     SRFGVGDDVSFYWSWPDELADGQRFVLYLVSAGERRPLGQVDETNLGRAYHLRVPVEE
                     TGEFRWEVVLEDAATGATIGASENRAISMIGS"
     gene            complement(5335..5973)
                     /locus_tag="PK829_00915"
     CDS             complement(5335..5973)
                     /locus_tag="PK829_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU39803.1"
                     /translation="MTKRFILVAGNIGVGKTSLTERLGARLGWVTGFESVADNPYLPD
                     FYADMRQWAFHLQVFFLGHRAQQHLLLAQSPQSAIVDRSIYEDAHIFARVLHHMNNLN
                     ERDYQAYRAVFELVVANLPRPDLLIYLTAPVPVLMERIRQRGRPIEAAITADYLSLLD
                     SFYQEWLAVFDLSPVLTLRTQQLDFVHHARHLDSVIDHIEATLAGREELILE"
     gene            complement(5970..6614)
                     /locus_tag="PK829_00920"
     CDS             complement(5970..6614)
                     /locus_tag="PK829_00920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU39804.1"
                     /translation="MSDPRTTVRLGYNRAAAAYLADRTLASPDVLALNHLLRHLSPGA
                     LVLDAGCGAGVPIAATLAAAHRVIGVDFSTAQLALARAHVPAAALACQDLTRLGFASN
                     VFDAVVSYYAIIHVPRAFHTSLLATFYRALKPGGYALLCLGAADLDDDFDDDYFGVRM
                     YWSHFDAPTNLGLLRAAGFEVLWHDFIADSLGDAPPGAGHLFVLARKPATIQTP"
     gene            complement(6718..6804)
                     /locus_tag="PK829_00925"
     tRNA            complement(6718..6804)
                     /locus_tag="PK829_00925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6768..6770),aa:Ser,seq:cga)
     gene            complement(6868..>7216)
                     /locus_tag="PK829_00930"
     CDS             complement(6868..>7216)
                     /locus_tag="PK829_00930"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOU39805.1"
                     /translation="GFNRKESDQDPTAHAEMIALRAAATHLGNWRLIGVTLYCTLEPC
                     PMCAGAMIQGRLARLVYGAKDTRFGADGTIIDVLGQPAFNHRVAVTTGLLAEEAGALL
                     QEFFRQLRGANGG"
     assembly_gap    7217..7316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7380..13475
                     /locus_tag="PK829_00935"
     CDS             7380..13475
                     /locus_tag="PK829_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU39806.1"
                     /translation="MSSQKSVRLFLILMALVVALAACRRGQTEAIPTIAATAAAEEPE
                     GAAQPTEPAAAATTAPATSATAEPVVARPVPVEAIDWAPQVVTSDPLPGGEIAANAPI
                     TVRFDQPMDQASVAAAFAIEPAVEGDLSWPEPAVAVFTPAEALAEGQTYHVRVADSAA
                     SVNGQTLLEPVDFALQTSGPLAVTQVSPEDGATAIGTDAAITVVFNKPVVPLVSSGEQ
                     ASLPQPLTFDPPVAGAGEWTSTSIYRFVADPALAGSTSYTVSVDPALTDLTGSVIENP
                     PTWQFTTLGPEVVTVEPENNAERIVPTTPITVTFNMPMNPTGVEGALSLSAGDEAVAY
                     TAEWQEGNRAVVLTPDDLLPLASDITLTVGASAPSANGGATLAGDHVSTFTTVPLPAV
                     VSTFPADGGTADVWQRGFSVEFASPMNDDTVVDQFVIEPAPGDVDYFVNEWEGNYSVS
                     LDFELTPNTTYTVTIPATAADPYGNTLGEDYVFTFNAPPFAPLASLNLPRDVAQLSTS
                     FPTTVQVLHRNVTEVTVSLYDLGVNTDLIFNSYQINEHQPSGEPVFQTTIPVENVPDA
                     LDVIDVPLADGGVLPTGVYELRVNAPEVDADSSYWQVRNVVLVVADTNIVVKEMFGEV
                     NVWVTDLATGQPVAGRELTLYKRSGEAAGTATSDANGFARFDYTPDEQYLEGVVVVSN
                     EPGAAGFGMASTLWTATVQPWQLGLTMDSGPEQSLYAYIYTDRPIFRPGDTVYYKGIV
                     RVPSFGRYLLPDIEELEISVNPNFFFGEDVDTFSQTFTVTLDEDGVFNGELTLPEDMP
                     LGSYSFNLTGNFWLSSRPFTVAEYRAPEFQVTVTPEQAEVLRGQATNVNVDVAYLFGG
                     SAAGLPVNWNVIESEYVPSFESAPAYVFGDAGDFNYEFDPFRFGGTETTVASGNGETD
                     AAGRVTIPLPADLLAEADAGSRTVRVEVTVGGLGEFPVSGRTEVIFHGADAYVGLRPQ
                     NNIVNAGDEAAVDLLTVDWAGEALGNQPVEVVFYQRDWESERVVEFGQRVTQWTPVDT
                     EVGRETVTTDEDGQATVSFTPASGGTYLVVATLTDAGGREQMASTAIWSVDAQFAGWR
                     TDPNQRTMDVTTDKREYRPGETAEILVQSPFAQPVQAWLVVERGNIIDQQVITVNGSQ
                     IISLPITEDYAPNVHVTVVAIKPVDSADAGFPYADIRVGFVEIVVPPDQFDLTVTLTP
                     GAEEYAPGDTATFDIAVTDQAGNGVQSEVSVALVDLAVLLLKEDNAPPILDAFYFRQP
                     LRSTLGSGLLITGEGLAIEEPLMAGGFGGGGGADELASSFRIEGEDDVRQDFRDTAYW
                     EARLMTDADGRATVEIPLPDNVTTWRLHSKATTTDTRVGQASVDILARLPLIIRPVTP
                     RFFTVGDQVSLGANVNNNTDADIEATITLEATGVTIDGEATQTVTVPAGGRQLVRWNV
                     TVDDVETADLTFRVEGGGFSDASKPTLGVGPDALLPVYRYDGRDFVATAGELDEEGRR
                     VEAVVLPQGVDQRQGEVQVRIQPSLAAAIIESFDVIEADNATWLECAGTYGDRLLYNA
                     SVEAAVRALELDRPDLQDRLTERVSADVAALQAAQFENGGWGWCFSAEADPWISAQAV
                     LALSRAAGLGYDVDAAVLDKGVAYLRTQLQPVGNLGDAAEANRQAFFLYVMAEAGENV
                     ADDADTLVGEHRGLLDPYAKALLAMAYDLSGATGANPTALLTDLNDTAIMSATGAHWE
                     SAEQDFLNLDSDIRGTAMVIAALARLQPDSPLLPPAVRWLMVARDAETWATLHQTAWS
                     VSALSDWMAVSGELEADYAYELLVNLQSRAAGSFTADNVTASDIVRVPLNELVGDDTN
                     FFDFQRGAGSGRMYYTLRLDSAIAVDALDPISRGFTVERRYFDAACDPAAETCEPIDT
                     IGIDGQVRVELTIVVPNDRVYVMVEDPIPAGTDAIDPNLLTSQSGRAGGIVPAEGDFA
                     RGFWGWWYFDHIQYRDEKVVFLSQFLPAGTYQYTYYLQPSTPGVYQAMPATAREQFFP
                     EVFGRSEGAIFTITE"
BASE COUNT         2312 a         4490 c         4534 g         2361 t
ORIGIN      
        1 ggtcgccgtc cacatcgtta aacgcgccga tgaagtcggc cgcggcgaag aggatgttga
       61 cgccctcgtc accggtcttc tggaaatcgt tcacggtcgg cttgtcgttt tgcgactgga
      121 ccgtgatcgt cacctgggcc gaggcggcgg cgtagttgct gccgtcggag gcgttccact
      181 caaacgaagt gctgccctcg ttccagttcg gcgcgggcac aaaggtgagg ttgcccaact
      241 cgccgacggg aatctccacg cccgacgtga tcgggctgtt gttgagcttc agcgtgccgt
      301 ccgatggcag cgagtcgatg cgcaccctaa ccagtgaatc gccgtcggcg tcgttaaagt
      361 gggaggtgaa cgtgttggcg gtgaagttga ccgccgtatc ctcgtcggtc gacagggcga
      421 tatcggtcag cgtcggcgcg tcgttggtgg ccccgaccgt aacctggaag gtcgtctgga
      481 cggtgccatt gtcagtgtcc ctggcctgga cggtcagtga cgccgtgccg aaggcgttgg
      541 ccttatagtc gagcgtcagc gtcagcgccg cgtcatcgac cgtcacgctc tggaacagat
      601 tttcattggt gttgttggtg acggagtagt ctagctcgtt gtcggcgtct tccttgtccg
      661 cgaaggccgg agcgaggttg atgacggtgt cgggcgcgtc ctcgttgacc gtcacgttgg
      721 cgatgccgct ggttgtcggg ttgtcgttga cggcgttgac cgtcaccttg aacgttgttt
      781 ccgcccacag cccgccgctg tcggtcgcgc gcactttgac gtcggacttg ccggccgcat
      841 cgggggcata gtcgagagtg aggttgggct tggtgatcgt cggggcgggg tcgaacaggg
      901 ccgggttgct gacgctgatg atggcgtagg tcaggtcggc gtcggcgctc tccgcgtcgt
      961 cgaagacatc atagaggttg atctgcgacg tgagggcgtc ctcgttgaca gtgacgttct
     1021 gcgtggccgg cccggtaggc gcgtcgttga tatcaacgac gaaggggtcg tcggtgtggg
     1081 catcctgtga gtcgaaggcg cgcacggtga tattggccac gcccgcgccg ctggcggcga
     1141 aggtcagggt cagcgtgccg gcgtcgttgt tgacctgcgc cgtcaccagg ctagggttgc
     1201 tgttattctg caccacaaag gtaagcgccg cgtcggggtc ctcgtcgtcg gagaagtatt
     1261 gccacaggtt gatcaccttc ggcgcgccgc cttcgttaac gacctggtcg gggatgcccg
     1321 tcgtcttggg ggggtcattg accggcgtga ggttgacggt gatggtggcc gagccggact
     1381 tgcctttctc gtccatcgcc tggacggaga aggtgatcgt cgggttggtc tcaaagtcca
     1441 gcagggcgct gttggccaca gtgatctggc cagaattagg atggatggcg aagatagtcg
     1501 tgccgctgag cgaataagtc agaggcccct tttcgggatc gtcggcgtca actttgccca
     1561 cgaccgcgcc attggcggca tcctcagcca gggtgaatgt ggccggggtg acgacggggt
     1621 tctcgttgag gtcggtgacg ttgatggtga tgagggctgt gtcggtcagg ccgccctcgt
     1681 ccttgatttc gaccgtcatg ttgtaggaat taacggcttc gtagttaagt tgcgcgcccg
     1741 gagccacggt aatcttgccg aggctgtcga tattgaacag gccattgccg ctgccgccgg
     1801 taatggtgaa ggtgtgcgag tcgttggtgt cgccatcggt atagaccaat gtgcccaccg
     1861 tcgtgccggc ggggctgttc tccgccaccg agaaggtctg gtcgcccatg accggcggct
     1921 gatcggaagc gtcgatcagg ttgatggtta ttgttgtctc cgcgctctca actggagcca
     1981 gggtgtcgtt cacgcggata ttcagctgga aagacttctt cacctcatag tctagcaagg
     2041 tgctgtcttc tacggtgata acgccggtag tgctgttgat atcaaaggca ttggcgccat
     2101 cgccgccaag gatatcgaag gttaccggct cgccctcggg gtcgaacgcg tctaacacgc
     2161 tgggggtagt cgacgaaccg ttggggctgt tttcaacaat cgaaaacttt tggtctggtg
     2221 tgatgagcgg ggcggcggca cgcgtcggcg tcgtcacaat cagcgccagc aaaaaggcgg
     2281 cgacaacaga tgcaatgctc aggtaggtgg cttgtctctt catgatgctc ctgactcctc
     2341 ggcgcgctgg ggtctgcgga acccaccagt catctctcaa acccgcaata gatagccagg
     2401 cagatgcctt gcaaccgggt ctaaaagaag acgccgaatc tatggtatcg tcacatagcc
     2461 tgatcgacgg ttaaaatgca gtgaaaagtc gattgtggct tcccccggcg cacaggcgcg
     2521 gcgggcgaca acaatgcacc acgaattgtg cccgcgcccg cacgaagacg cggcacttct
     2581 catatggcgc ttacgcgggt gcaagacaag cggcatcccc gccccccgtt aaggctgctg
     2641 accgggcggt gtcttggtgg gtagttccgg cggcggtgtg tccgtcggtt gttccggctg
     2701 tggcgtgttg gtgggcactt ctggctgtgg cgtattggtt ggctgctgtg gcggcggggc
     2761 gctcgtcgct gttggtgatg tcggcgtcgg cgtccgcggt acggccgcca gcggtaccca
     2821 cacctgttgg cccacgctta acaggtcgtt gggcaggcag ttgacctgga gcagcgactc
     2881 aacggggatg ttggcccgct cggccaggtt cgacagcgag tcaccctggc gaacggtgta
     2941 gagcacccag ccgggcggcg gtgagacgac gcaggcttcg gccagcgtcg gcgtggccgt
     3001 cggcgtctgc ggcgcgggcg tgccggtggg cgagggcgtg ggggttagct cgcgaatgat
     3061 aatgggaatc acgtcgctgg ccacttctac cccaccgccg ttaacattct ccagtcttac
     3121 ttgccagccc aaatcctcgc cggtgacagg gatatcggcg ccgtccacag tcagacgata
     3181 ggagatacca ttgttcggct cggttagggt gccgagcttg tgttcctggt cgccgtcgat
     3241 caggtaaagg gcgaagtgct ggcccggttc cggcagggca ggccaatacc agtcgaagat
     3301 aattgtatcc gcgatggtga acgcgcggcc ggggccaggc gagagtgccg ggatggccac
     3361 ttcgccggca gactccgtcg aaaactcgat ggtagccgtg gtcgtgggcg atggcgtcac
     3421 gtcggggttg gctgtggcca cgccggccac gtcgttaggc gtcgtttcgg ccacaggcgt
     3481 atcttcagcc tcggcctgtc cgctgcgcgc gttgcgcaag atcagaaagc ccagcaggcc
     3541 cagcaccaac agggccaccg gaatgatcac caggaaggaa cggtttggct tgggcgtcca
     3601 ggtggccgcc ttgggcaact cctgctcggc gaaaatggcc gtctcgatgg cagtgatcaa
     3661 ctcgtcggcc gtctcaaagc ggctccactc ctgccgccac aggcagttgc gcaccagccg
     3721 gtaggtctcg ccagccaggc cggggcgcgc ttcctccaag ggctcttcct tgggcatatt
     3781 ggcctgcggg aagatgtcga aaggcaatac cggcaacttg ggccggtggc cggacagcag
     3841 ctcgtagagg atgacgccaa aggagtagat gttgctgcgc cgggttatgc ctttgccttc
     3901 cagctcttcg ggcgaggcgt agtcaagctc ctcgctctcg gagccattga cgcccggagc
     3961 ggcgggatcg aggacgaggg gcaagcccag gtcgatcaag acaggcgtgc cgtcctcgcg
     4021 cagcatgacg ttttctgggc gcaagtcgtg gtgaatcagg ccggcggcgt gggccacgcc
     4081 caacgcctga gccaggtgtt tggccaattc cagtgccgcg atgaccggca gcttatcctg
     4141 ggcgcgggcc aggtcggcca gcttgtcggc cagcgtgacc ccggggaagt agtcgatgac
     4201 cgcatagggg aagccgtcat cggtgatgtc aagatcgctg acgacagcaa tggatgggtc
     4261 cttaagctgc gagacggtct ccatgcgccg gcggaagcgc gcggccagat tctcgtcctc
     4321 ttcggttgtc gtgcgcagca cctctagaaa gacgaggcgc tccagcttga cgtcacgcgc
     4381 gagaaagaga tcgctggctt tgcgcgccgc gatatgagat tcaatctcgt agttacctat
     4441 ctgttgccca gacaggtctt ccttatccct tgccaccatg ctcagcttac ttgtgcgcgc
     4501 cctctcaggt cgttttacag gtcaatcggc gggccaggca tggcacctcc gatggcccta
     4561 tattcgctct agcgtactat tttacacaac aaaatgcaat ggttccgggc gacaatcacc
     4621 ctcgccgacc acctcaggcc cgccgtcttg cccgcgccaa tcagctaccg atcatgctga
     4681 tggcccggtt ttcactggcg ccaattgtcg cgccggtcgc tgcatcctcc aggacgactt
     4741 cccaccggaa ctcgcccgtt tcctcgacgg gcacgcgcag atggtaggcg cgccccagat
     4801 tcgtctcgtc tacctggccc agcggccggc gctcgccggc gctcaccaga tacaggacga
     4861 aacgctgtcc atcggccaac tcatccggcc aactccagta gaacgacaca tcgtcgccca
     4921 cgccgaagcg gctgcccgcc ggcggaccat acagggcgat ggctcccggc gcaaccacgg
     4981 caaccgtcgc ggtggccgtc ggctccagcc cggccgcgcc ccatggcgtg gcctgtccga
     5041 tcaaaccctg cggcggggcc gtcgatgcgg ccatcggcga cgcagccgtg gctgacgccc
     5101 gcatcggcgt caccaccgtc acggctgtcg gtctggccac ggccgactgc gagagccagt
     5161 accagccgcc aatggccagc aacagcagca gcggcgttgc caggcgcgcg gctttgcctc
     5221 cgctacccat ggtagacatg cccacgccgc ctggtagcct cagacgccat catcatactt
     5281 gtgaattcta gcacaagacg ggcgtgtcga aaacggactt ttgtccagag aagattactc
     5341 gaggataagt tcttcgcgcc ccgccagcgt cgcctcgatg tggtcaatga cgctgtcgag
     5401 atggcgggcg tggtggacga agtcgagttg ctgggtgcgc agggtcagca ccgggctgag
     5461 gtcgaagaca gccagccact cctgatagaa ggaatcaagc agagagaggt aatcggcggt
     5521 gatggccgcc tctatgggtc ggccgcgttg gcggatgcgt tccatcagca cgggcacggg
     5581 cgcagtcaga tagatgagca ggtcgggccg cggcaaattg gccacgacca gttcaaagac
     5641 ggcccgatag gcctggtagt cgcgctcgtt gaggttgttc atgtggtgca gcacgcgggc
     5701 gaagatgtgg gcgtcctcgt agatgctgcg gtcaacgatg gccgattggg ggctttgggc
     5761 caggagcagg tgctgctggg cgcggtggcc gaggaagaag acctgcaagt ggaaggccca
     5821 ctggcgcatg tcggcgtaga agtcgggcag gtagggattg tcggccaccg actcgaagcc
     5881 ggtgacccag cccaggcgcg cgcccaggcg ctcggtcagc gaggtcttgc ccacgccgat
     5941 attgccggcc accaggatga agcgcttggt catggagtct gaattgtagc cggtttgcgc
     6001 gccaacacga aaagatgacc cgcgcccggc ggcgcgtcgc ccagcgagtc ggcgatgaag
     6061 tcgtgccaga gaacctcgaa gccggccgcg cgcagcaggc ccaggttcgt cggcgcgtcg
     6121 aagtggctcc agtacatgcg cacgccgaag tagtcgtcgt cgaagtcgtc gtcgaggtcg
     6181 gccgcgccca ggcacaacaa ggcgtagccg cccggcttca gcgcccggta gaacgtggcc
     6241 agcagactgg tatggaacgc gcgggggacg tggatgatgg cataatagga aacaacagca
     6301 tcgaagacgt tggacgcgaa gccaagccgg gtcaagtcct ggcacgccag cgccgccgcg
     6361 ggaacatgcg cgcgggccag cgccagttgc gccgtcgaga aatcgacgcc gatcacgcgg
     6421 tgggcggccg ccagtgtggc ggcgatgggc acgcccgcgc cacagccggc gtcgagcacc
     6481 aacgcccccg gtgacagatg gcgtagcaag tggttgaggg ccagcacgtc gggcgaggcc
     6541 agtgtccggt cggccaggta ggcggcggcg gcacggttgt agcccagacg cacagttgtg
     6601 cgaggatcgc tcattgggca agtgtatctt cagatgggca caagccccag ggatcgcgcc
     6661 ggcgggcagg accaggacat agagaaaagc ccccaggcaa aggccggggg ctggcagcgg
     6721 agagggtggg attcgaaccc acggtgacct cgcggccaca acgcttttcg agagcgcccc
     6781 gatcaaccac tctggcacct ctccagcaga actcaagtat agcagcaatt agcctatggc
     6841 aacagggcca acgcgccgct ggcttcgtta gcccccattg gcgccgcgca attggcggaa
     6901 gaactcttgc agcaacgcgc cggcctcctc ggccagcagg ccagtggtga ccgcgacgcg
     6961 gtggttgaac gccggttggc caagcacgtc gatgatcgtg ccgtccgcgc cgaagcgtgt
     7021 gtctttggcc ccgtagacca gccgcgccag ccgcccctgg atcatcgccc cggcgcacat
     7081 ggggcacggc tccagcgtgc agtagagcgt cacgccgatg agccgccagt tgcccaggtg
     7141 agtggcggcg gcgcgcaggg cgatcatctc ggcgtgggcg gtggggtcct ggtcgctctc
     7201 cttgcggttg aagcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatct
     7321 gccgctggcg cgcacctgcg cgccgcggcc catttcccgc catcgcggag gtttgtcata
     7381 tgagttccca gaagtccgtg cgcttattcc tgatcctgat ggcccttgtt gtggccctgg
     7441 ccgcctgccg gcgcggccag acggaagcga tccccacgat cgcggccacg gccgccgccg
     7501 aggagccgga gggcgccgcc caacccaccg agccggccgc cgcagccacg accgcgccgg
     7561 cgacctcggc cacggccgaa cccgtcgtgg cccggcccgt gcccgtcgaa gccatcgact
     7621 gggcgccgca ggtcgtcacc agcgatcccc tgcctggcgg cgaaatcgcc gccaacgcgc
     7681 ccatcaccgt ccgcttcgac cagccgatgg accaggcgtc cgtcgccgcg gccttcgcca
     7741 tcgagccggc cgtcgagggc gacttgagct ggccggagcc ggccgtggcc gtcttcacgc
     7801 cggccgaggc gctggccgag ggtcagacct accacgtgcg cgtcgccgat tcggccgcct
     7861 ctgtcaacgg gcagaccctg ctggagccgg tcgatttcgc tttgcagacc agcgggccgc
     7921 tggccgtcac ccaggtcagc cccgaagacg gcgcgacggc catcggcacc gacgcggcga
     7981 tcactgtcgt cttcaacaag ccggtcgtgc ccctggtcag cagcggcgag caggccagcc
     8041 tgccccaacc gctgaccttc gacccgcccg tcgcgggcgc aggcgagtgg acgtccacca
     8101 gcatctaccg cttcgtggcc gatccggccc tggccggttc gacgagctac accgtcagcg
     8161 tcgatccggc tctgaccgac ctgaccggca gcgtcattga gaacccgcca acgtggcagt
     8221 tcacgacgct tggcccggag gtcgtcaccg tcgagccgga gaacaacgcc gagcgtatcg
     8281 tgccgacgac gcccatcacc gtgaccttca acatgcccat gaacccgacc ggcgtcgaag
     8341 gcgcgctgag cctgagcgcc ggcgacgagg ccgtggccta cacggccgag tggcaggagg
     8401 gcaaccgcgc cgtcgtgctg acgcctgacg acctgttgcc gctggcgtcg gacatcacgc
     8461 tgaccgtcgg cgcgagcgcg ccgtcggcca acggcggcgc gacgctggcg ggcgaccacg
     8521 tcagcacctt cacgaccgtg cccctgccgg cggtcgtctc gaccttcccg gccgatggcg
     8581 gcacggccga cgtctggcag cgcggcttca gcgtcgagtt cgcctcgccc atgaacgacg
     8641 acacggtcgt cgaccagttc gtcatcgagc cggcccccgg cgatgtcgat tactttgtca
     8701 acgagtggga aggtaactac tccgtctcgc tggactttga gttgacgccc aacaccacct
     8761 acaccgtgac catcccggcc acggcggccg acccctacgg caacacgctg ggcgaggact
     8821 acgtcttcac cttcaacgcc ccgccctttg ccccgctggc gtcgctcaat ctgccgcgcg
     8881 atgtcgccca gttgagcacc agcttcccga cgaccgtaca ggtcttgcac cgcaacgtca
     8941 ccgaagtcac ggtgtccctg tacgacctcg gcgtcaacac cgatctgatc ttcaactcct
     9001 accagatcaa tgagcaccag ccgtctggcg agccggtctt tcagacgacc atcccggtgg
     9061 agaacgtgcc cgacgcgctg gacgtgatcg atgtgcccct ggccgatggc ggcgtgctgc
     9121 ccaccggcgt ctacgagttg cgcgtcaacg cgcccgaagt agacgcggac agcagctact
     9181 ggcaggtgcg caacgtcgtg ctggtcgtcg ccgacaccaa catcgtcgtt aaggagatgt
     9241 tcggcgaggt caacgtctgg gtgactgatc tggccaccgg ccagcccgtc gccggccgcg
     9301 aactgacgct ctacaagcgc agcggcgagg ccgccggcac ggccaccagc gacgccaacg
     9361 gcttcgcccg cttcgactac acgcccgatg agcagtatct cgaaggcgtg gtggtcgtca
     9421 gcaacgagcc gggcgcggcc gggttcggca tggcctccac actgtggacg gccaccgtgc
     9481 aaccctggca actgggcctg accatggaca gcggcccgga gcaatcgctc tacgcctaca
     9541 tctacaccga ccggcccatc ttccgcccgg gcgacacggt ctattacaag ggcatcgtcc
     9601 gcgtgcccag cttcggccgc tacctcctgc ccgacatcga ggagctggag atctcggtca
     9661 accccaactt cttcttcggc gaggacgtag acaccttcag ccagaccttc actgtcacgc
     9721 tggacgagga cggcgtcttc aacggcgagt tgacgctgcc ggaggacatg ccgctgggca
     9781 gctactcctt caatctgacc ggcaactttt ggctctccag ccggcccttc actgtggccg
     9841 agtaccgcgc gccggagttc caggtcactg taacgccaga gcaagccgag gtgctgcgcg
     9901 gccaggcgac gaacgtcaat gtggacgtgg cctacctctt tggcggctcg gccgccggcc
     9961 tgccggtcaa ctggaacgtc atcgagtctg agtacgtgcc ctcgtttgag tcggcgcccg
    10021 cctacgtctt cggcgacgcg ggcgatttca actacgagtt cgatcccttc cgctttggcg
    10081 gcaccgagac caccgtcgcc agcggcaacg gcgagaccga cgccgccggc cgtgtgacca
    10141 ttcctctgcc ggccgacttg ctggccgagg ccgacgccgg cagccgcacg gtgcgcgtcg
    10201 aagtcaccgt cggcggcctg ggcgagttcc ccgtcagcgg ccggacggag gtcatcttcc
    10261 acggcgccga cgcctacgtc ggcctgcggc cccagaacaa catcgtcaat gccggcgatg
    10321 aggccgcggt cgatctgctg accgtcgact gggccggcga agcgctgggc aaccagccgg
    10381 tcgaggtcgt cttctaccag cgcgactggg agagcgagcg cgtcgtcgag ttcggccagc
    10441 gcgtgacgca gtggacgccc gtcgacacgg aggtcggccg cgagaccgtg accaccgacg
    10501 aggacggcca ggccacggtg agcttcacgc ccgcatcggg cggcacgtac ctcgtcgtgg
    10561 ccacgctaac cgacgccggc ggccgcgagc agatggccag cacggccatc tggtcggtgg
    10621 acgcccagtt cgccggctgg cgcactgacc ccaaccagcg gacgatggac gtgacgacgg
    10681 ataagcgcga gtatcgcccc ggcgagacgg cagaaatcct cgtccagtcg cccttcgccc
    10741 agcccgtgca ggcgtggctg gtggtcgagc gcggcaacat catcgaccag caggtcatca
    10801 ccgtcaatgg cagccagatc atctcgctgc ccatcaccga ggactacgcg cccaacgtcc
    10861 acgtgaccgt tgtggccatc aagccggtcg actcggccga cgccggcttc ccctacgccg
    10921 acatccgcgt cggcttcgtg gagatcgtcg tcccgcccga ccagtttgat ctgaccgtga
    10981 cgctgacgcc cggcgcggag gagtacgccc ccggcgacac ggccacgttc gacatcgccg
    11041 tgaccgacca ggccggcaat ggcgtccagt ctgaggtttc cgtggccctg gttgacctgg
    11101 ccgtgctgtt gctgaaggaa gacaacgcgc cccccatcct cgacgcgttc tacttccgcc
    11161 agccgctgcg cagcacgctc ggctccgggc tgctcatcac cggcgaggga ttggcgatcg
    11221 aggaaccgct catggccggc ggctttggcg gcggcggcgg ggcggatgag ctggcgtcgt
    11281 cgttccgcat cgaaggcgag gacgacgtgc gccaggactt ccgcgacacg gcctactggg
    11341 aagcccggtt gatgaccgac gccgacggcc gcgccacggt cgagatcccg ctgcccgaca
    11401 acgtcaccac ctggcggctg cacagcaagg cgaccaccac cgacacccgc gtcgggcagg
    11461 ccagcgtcga tatcctggcc cgcctgccgc tcatcatccg cccggtcacg ccgcgcttct
    11521 tcaccgtcgg cgaccaggta tcgctgggcg cgaacgtcaa caacaacacc gacgccgaca
    11581 tcgaggccac gatcacgctg gaagcgaccg gggtgacgat tgacggcgag gcgacgcaga
    11641 cggtcacggt tccggccggc ggccggcagt tggtgcgctg gaacgtcact gtcgatgacg
    11701 tggagacggc cgacctgacc ttccgcgttg agggcggcgg cttcagcgac gccagtaagc
    11761 ccaccctggg cgttggcccg gacgcgctgc tgcccgtcta tcgctacgac ggccgcgact
    11821 tcgtggccac cgccggcgag ctggacgagg aaggccgccg cgtcgaggcc gtcgtcctgc
    11881 cgcagggcgt ggatcagcgc cagggcgagg tgcaagtgcg catccagccc tcgctggccg
    11941 cggccattat cgagtcgttc gatgtcatcg aggccgacaa cgccacctgg ctggagtgcg
    12001 ccggcaccta cggcgaccgg ctgctctaca acgcctcggt tgaggcggcc gttcgcgcgc
    12061 tggaactcga ccggcccgat ctgcaagacc ggctgacgga gcgcgtcagc gccgatgtcg
    12121 ccgccctgca ggcggcccag ttcgagaacg gcggctgggg ctggtgcttc agcgccgaag
    12181 ccgatccgtg gatcagcgcc caggccgtgc tggccctgtc gcgcgcggcc ggcctcggct
    12241 acgacgtgga cgccgctgtg ctggacaagg gcgtggccta cctgcgcacc cagctacagc
    12301 ccgtcggcaa cctgggcgac gccgccgagg ccaaccggca ggcgttcttc ctctacgtga
    12361 tggctgaagc cggcgagaac gtcgccgacg acgccgacac gctcgtgggc gaacaccgcg
    12421 gcctgctcga cccgtatgcc aaggcgctgt tggccatggc ctacgacctg agcggcgcga
    12481 ccggcgcgaa cccgacggcg ctgctgaccg acctgaacga caccgccatc atgagcgcca
    12541 ctggcgcgca ctgggagagt gccgagcagg acttcctgaa tctcgacagc gacatccgcg
    12601 gcacggccat ggtcatcgcc gcgctggcgc gcttgcagcc ggacagcccg ctgctgccgc
    12661 cggccgtgcg ctggctgatg gtcgcccgtg atgccgagac gtgggccacg ctgcaccaga
    12721 ccgcctggag cgtcagcgcc ctcagcgact ggatggctgt ctccggcgaa ctggaagcgg
    12781 attacgccta cgagctgctg gttaacctgc aatcgcgggc ggcgggcagc ttcacggccg
    12841 acaatgtcac cgcgtcggac atcgtccgcg tgccgctcaa cgaactggtg ggcgacgaca
    12901 ccaacttctt cgacttccag cgcggcgcgg gcagcggccg gatgtactac accctccgcc
    12961 tcgacagcgc catcgccgtt gacgctctcg acccgatcag ccggggcttc accgtcgagc
    13021 gccgctactt cgacgccgcc tgtgatccgg cggccgagac gtgcgaaccc atcgacacga
    13081 ttggcatcga cggccaggtg cgcgtcgaac tgaccatcgt cgtgcccaac gaccgggtct
    13141 acgtcatggt cgaagacccc atccccgccg gcacggacgc catcgacccc aacttgctga
    13201 ccagccagtc ggggcgcgcc ggcggcatcg tgcccgcgga gggcgacttt gcccgcggct
    13261 tctggggctg gtggtacttc gaccacatcc agtaccgcga cgagaaagtc gtcttcctga
    13321 gccagttcct gcccgcaggg acgtaccagt acacctacta cttgcagccg agcacccccg
    13381 gcgtctatca ggcgatgccg gccacggcgc gcgagcagtt cttcccggag gtcttcggcc
    13441 gctccgaagg cgctatcttc accattactg agtaggctat gtgggcgtgg gcctcatacc
    13501 ccgcgcccac cgcaccacac ggcgggggcg agacattcgg atagatgtct cgcgacctga
    13561 caggtgagcg accgctcacc tgtcaggtcg gtgcgcctgg ggtgactttg gaaaagccct
    13621 ggaagtcctg cgccaatcgt tgaacgcctg ggccaagttc tgctacacta ctgtcggcat
    13681 gtctcgactg cgccccacac tctatctgct gctcctgctg gccctcggtt tgctgctggc
    13741 tgcttgtggc ggcgacaacc cgcccctggc cgtggaagag gccgccaaag tcatgtg
//
LOCUS       DAOSMQ010000013        13713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_4498_length_13713_cov_4.783131, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000013 DAOSMQ010000000
VERSION     DAOSMQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13713
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4498_length_13713_cov_4.783131"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PK829_00940"
     CDS             complement(<1..1635)
                     /locus_tag="PK829_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39807.1"
                     /translation="MRPTFTARRLLLFLLINAAFIAFLSLWNRQLHRESVQLAVAKSK
                     QMLDMPAEVAGYPAGYALDTAAVRAVAQADMTLAALLADRSTEFVGAVRLSAGEARYW
                     QDQGCDQLNCAIVTYYNYTDGGTIEAIVDLDRDRVIAQWQDADARPGGSSEVLPKAMR
                     IAAADAQVRAILGDDIGAMDPAMIPMSGWLADNACREEWCVDLTFHDPAGTGRVYHVF
                     VNLEREVVARTFYTRARADRSAGKPVAQRNAFTDGCHEQYGWNVCWEMTANDGINFRD
                     ATYSGNNIFSSAKIGQIEAWYPSWPGGYRDEIGFAATVPPFGDTLVNDLGDGFEVRQL
                     FTEFTRWPNCICCYRYEEIMRFYSDGRFQPRFISHGPGCDDLSVYRPFWRIDVDLDGG
                     PGDQVWLFEDTQWVEQKVEFETHPVVEGLSPEGHKLAIFDGDVHYRWFMERTDPLGLD
                     EAYFFLLQDKPLEGEEPIVTGPGDTFIPPRQWIDGDALSGQDVVLWWVPLLHTKKGGP
                     WWCMPDPEPDFSPCEAILHAEPAGELRQPTAEELAAL"
     gene            1876..3099
                     /gene="coaBC"
                     /locus_tag="PK829_00945"
     CDS             1876..3099
                     /gene="coaBC"
                     /locus_tag="PK829_00945"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOU39808.1"
                     /translation="MQAITVFQGRRILLGVTGSIAAYKSVDLASKLTQAGALVDVVMT
                     ESAARFVTPLAFRSVTGRPVYTDMWSLDDHVRHVGLGEAADLFLIAPATANTLAKLAH
                     GLADNLLTVTALAARCPVVVAPAMDGGMYEHAATQANLAALAARGVVLAGPAEGRMAS
                     GLSGLGRMLEPETLLGEARRILARDGVLSGRRIVVTTGPTREALDPVRYISNRSTGKQ
                     GVAVAQAALDAGAEVTLIAGPIAASPPTGARVIPVETTQQMGDAVLGCVADADALFMV
                     AAVADFRPETISDRKIKKTERPDWGLAIGLEKTLDILSAVKEQRDRTGYPRVVLGFAA
                     ETHNAFEYGRDKLLRKGLNFIAVNDVTAAGAGFAVDTNQVVLLSSAGVVETMPLQSKT
                     AIAERLIHHVAAALG"
     gene            complement(3096..4106)
                     /gene="bmt"
                     /locus_tag="PK829_00950"
     CDS             complement(3096..4106)
                     /gene="bmt"
                     /locus_tag="PK829_00950"
                     /EC_number="2.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF005718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine--homocysteine S-methyltransferase"
                     /protein_id="HOU39809.1"
                     /translation="MDKLQQLLDTHETIVLDGAMGTVLFAAGLQAGAPPEAWNVLHPE
                     RIRAVHRGYIRAGSDVILTNSFGGTRYRLKLHNLQDRVYELNRAAAQHAQAEAGAAGR
                     PVLVAGSMGPTGELLAPMGSMTFDDARAAFAEQARGLVAGGVDLLWVETMSDLEEARA
                     AVEGARAVSDLPIAATMSFDTNGRTMMGVTPAQAVQTMKEWGLVAIGGNCGNGIEEIE
                     GVIHAMHRQAPEVRLIAKANAGIPQWRHNELSYDATPPTMGRYARRVRALGACFIGGC
                     CGNGPDHILAIAEALRTPLTDVERAALDAELGAELEAKLKAATNDRSAAARARTRRRP
                     RA"
     gene            complement(4113..4808)
                     /locus_tag="PK829_00955"
     CDS             complement(4113..4808)
                     /locus_tag="PK829_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOU39810.1"
                     /translation="MNDEEIDLQAMSDDEIYELMKEDLYDGYADEIKDEVQVMLARGH
                     EPYAILTNGLVAGMDIVGADFRDGILFVPEVLMAAKAMKAGMAILRPLLAETGAERIG
                     TMVVGTVKGDIHDIGKNLTAMMLEGAGFEIIDLGVNNSVDNFLAAIEKHKPDILGMSA
                     LLTTTMPYMRVVIEALKDAGIRQDIIVLVGGAPLNQAFAEEIEADAYCRDAAVGVETA
                     KRFMAIRRQKAQA"
     gene            complement(4923..7013)
                     /locus_tag="PK829_00960"
     CDS             complement(4923..7013)
                     /locus_tag="PK829_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOU39811.1"
                     /translation="MTTANGEFQVIFMPSGRRGRVPAGQSLLDAARQMGVDIESICGG
                     RLTCAKCRVRVEEGDFAKHGIRSSAAHLSPAAARERDLLASAGDAASLASADGAAGRL
                     ACAAHVAGDLLITVPEESRAHKQIIRKSATARRIDIDPAIRQVYVEVDRAELGEHRGD
                     WGRVQAALAAQWGLSDLRIDLPALRRLQPALRAGNWAVTVLVWNEREVIDVQPGYKEG
                     IYGLAVDIGSTTIAGHLCDLRTGAVLATEALMNPQVAYGEDLMSRVAYAMTHAGGLDK
                     MHAAIIEALNALAVRAVRSAGLHTRDIHDAVFVGNTTMIHILLGINPQELGGAPFALA
                     TRDAVDVKARELGLRFHPGAAIHVLPAEAGHVGADNVGVLLAEEPHRQDELTLLVDVG
                     TNAEIVLGNRGRLLSASSPTGPAFEGAQIRFGMRAAPGAIERVRIDPADWTARFRVIG
                     EERWSDAWPTGVDALPDSQPRHLATGICGSGIIEAVAEMYLAGVLRGDGRFSDDRPHP
                     RLLWQDKKAAYVLATGDQTANGQPILVTQDDVRAIQLAKAALYAGAKLLMNRMGVERV
                     ERIVLAGAFGSVIDPLYAMLLGLIPDCPLERVVAVGNAAGDGARIALLNRHKRDEAAA
                     LARAVVYVETAIDPRFQDEFVSAIHIPHAADRFPHLESRLGAPLPRAAAGQGDTGRAR
                     RAERIRRRAGGARL"
     gene            complement(7205..7504)
                     /locus_tag="PK829_00965"
     CDS             complement(7205..7504)
                     /locus_tag="PK829_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HOU39812.1"
                     /translation="MTTYQILYWHDIPVQVRAGGRRDRVSVELPARFAAAVDSAAMAA
                     GLTGTDGYLSGFAWSAAEERDGSPVEVAAAVTAELDAHHTTIDWQATVAAIRKGS"
     gene            complement(7541..8884)
                     /gene="solA"
                     /locus_tag="PK829_00970"
     CDS             complement(7541..8884)
                     /gene="solA"
                     /locus_tag="PK829_00970"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HOU39813.1"
                     /translation="MTAYSKHYHAIVVGVGGMGSATCYQLARRNKRVLGIERFDIPHG
                     LGSSHGYTRIIRLAYYEHIAYVALLRRAYELWADIERIGGEQLLYRTGSLDCGPADSD
                     VFTGSLRSCIEYDLPHEILTSAQVSERYPAYRLPRENLAVFQPDGGFLLPERSTVAFV
                     EAAHALGAEIHGREALLGWEPWGDAVRVVTDRDRYTADTLVFTAGAWAQNLLPFLDGL
                     AVPERQVLAWLQPARPEWFRPERFPVFNCLVPEGRYYGFPVFAVPGFKFGRYHHFGET
                     GEAEYLSREAYREDEDVLRDFAARYFPDGAGPTMMLKACLFTNTPDRHFLIDLHPEYP
                     QVSFAAGFSGHGYKFASVIGEIMADLAERRATRHDISLFNLARFTGQVTALHQPKIDM
                     LARGAWSPADDGPTHPLYRGGGREVPGGALGQPLSPRQPGADSPPAAGDTTDPRQ"
     gene            complement(9023..10804)
                     /locus_tag="PK829_00975"
     CDS             complement(9023..10804)
                     /locus_tag="PK829_00975"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOU39814.1"
                     /translation="MNHASKRLNPVPSDLDIAQAATPLPIKEVAAQLGLTEDDLIYYG
                     KTKAKVHLDTLPKLANNPMGKYIDVTAITPTPLGEGKTTTSVGLVQGLGRLGHRAIAC
                     IRQPSMGPTFGIKGGAAGGGYSQVIPMEDFNLHLTGDIHAITAAHNLVAAAIDARWYH
                     EDRMNDERLAKVGLSRLNIDPYRVTWNRVLDVNDRALRNIIVGLGPEEDGRPRQTGFD
                     ITVASEIMAILALVNGDDYKSALRDLRQRAGRTVIGTNKDGKPITLDDLGVGGAVTVL
                     MKDALHPNLLQTLEGQPAFVHAGPFANIAHGNSSILADRLALRLGDYVVTESGFGADI
                     GMEKFFDIKCRTSGLVPNCVVLVATIRALKMHGGGPRVIPGRPLDKAYTDENVALLEK
                     GMANMVAHIRNAKRFGVPVVVAVNKFHTDTPAEIAAVERAAMAAGAETAVMTDHWANG
                     GDGAMALAEAVVAACEKPSNFQFLYPLDLPIKAKIETIATEIYSAGEVIYEPQAEKQI
                     KSYEDNGFGNLPICMAKTHLSISHDPLLKNAPSGYTLTVREVRASVGAGFIYPLLGEM
                     RTMPGLGTRPAYMNVDVDEDGRVMGLF"
     gene            complement(11012..11560)
                     /locus_tag="PK829_00980"
     CDS             complement(11012..11560)
                     /locus_tag="PK829_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU39815.1"
                     /translation="METSVIISGFGGQGALFAGQLLAYAGMDAGRFVSWIPSYGPEMR
                     GGTAHCTVIISDDEIGAPVIAQPDIAIALNLPSFDKYEPLVKPGGLLVVNSSLVASES
                     RRTDIDIVYVPANGIAEEWGTPKMLNMAAVGAMLAARAVLPLAVIEAALAAHLPAGKQ
                     HLIEANRQVLRRGFAVGQPAQV"
     gene            complement(11573..12898)
                     /locus_tag="PK829_00985"
     CDS             complement(11573..12898)
                     /locus_tag="PK829_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU39816.1"
                     /translation="MTTPWNQRCAQRMQWMSASTIRELLKLIENPEIISFAGGMPAPE
                     VFPVESFRAAADRVLAREGRRALQYGPTEGYRPLREWIVQQMARYGIESAPENVLITS
                     GSQQALDLIGKIMINPGDLILTERPSYMGALQAWRAYQAEFVSVPTDADGLRVDLLEE
                     ALCAGPKFMYILPNFQNPGGITLSRERREQLIAIADHYGVPIIEDDPYGELRYEGDHL
                     PPLVVIDAERLKGRAPQGEVYYGDDAHNGNGRAYMRGNVIYLSTFSKTLAPGLRLAWL
                     VAPVEVVQRCVVAKQGVDLHTATLNQMLAYEVLTGEDDFLDKHVRRIRAVYRERRDVM
                     LAALETYFPDEVHWTYPQGGLFLWVTLPEGLDAAKLLKDALAHQVAYVPGGAFHPEGG
                     GDNTLRLNFSLCDPQRIETGIQRLGEVFAQAITAQAHAGELVPALQPAF"
     gene            complement(12943..13695)
                     /locus_tag="PK829_00990"
     CDS             complement(12943..13695)
                     /locus_tag="PK829_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU39817.1"
                     /translation="MVYERPHALTDVHTHYCPGCTHGIAHRLVAEVIDELGIREDTIL
                     VASVGCSVFAYNYFEVDSCEAAHGRAPAMATGVKRVHPDKIVFTYQGDGDLASIGMGE
                     IMHVAARGERITTIFINNAVYGMTGGQMAPTSLLGQTTTSSPFGRDLRLTGAPIHMAE
                     LLAQMAGTAYAVRRSLHDARHIIQAKKAIRTAFEAQQKGLGFSVVELLSTCPTNWGMT
                     PADSLAWVRDSMIPVYPLGDYKVAPELALARR"
BASE COUNT         2155 a         4432 c         4752 g         2374 t
ORIGIN      
        1 cagcgcggct agttcctcag ccgtcggctg gcgcagttcg ccggccggct cggcatggag
       61 gatggcctca cagggggaga agtccggctc ggggtcgggc atgcaccacc acggcccgcc
      121 cttcttcgtg tgcagcaacg gcacccacca gagcaccacg tcctgaccgg acagagcgtc
      181 gccgtcgatc cactgtcgcg gcgggatgaa cgtatcgccg gggccggtga caattggctc
      241 ctcgccttcc agcggcttgt cttgcagcag gaagaagtag gcctcgtcta gccccagcgg
      301 gtcggtgcgc tccatgaacc agcggtaatg cacgtcgccg tcgaagatgg ccagcttgtg
      361 cccttccggc gatagaccct cgacgacggg gtgcgtttcg aactcgacct tctgttcaac
      421 ccactgcgtg tcctcgaata gccagacctg gtcgcccggc ccgccgtcga gatcgacgtc
      481 gatgcgccag aaggggcgat agaccgacag gtcatcgcag ccggggccgt gggagatgaa
      541 gcgcggctgg aagcggccgt cgctgtagaa gcgcatgatc tcctcgtagc ggtagcagca
      601 gatgcaattg ggccagcggg tgaactcggt gaagagttgg cgcacctcga agccatcgcc
      661 caggtcgttg accagcgtgt cgccaaaagg gggcactgtg gcggcgaagc cgatctcgtc
      721 gcggtagccg cccggccagc tgggatacca cgcttcgatc tggccgatct tggcgctgga
      781 gaagatgttg ttcccgctgt aggttgcgtc gcggaagttg atgccgtcgt tggcggtcat
      841 ttcccagcag acgttccagc catactgctc gtggcagccg tcggtaaagg cgttgcgctg
      901 ggccacgggc ttgcccgcgc tgcggtcggc gcgggcgcgg gtatagaacg tccgcgccac
      961 gacctcgcgc tccagattga cgaagacgtg atagacgcgg ccggtgcccg ccgggtcgtg
     1021 gaaggtcagg tcgacgcacc actcctcgcg gcaggcattg tcggccagcc agccggacat
     1081 ggggatcatg gccgggtcca tcgcgccgat gtcgtcgccg aggatggccc gtacctgggc
     1141 gtcggccgcg gcgatgcgca tggctttggg cagcacttcg ctggagccgc cggggcgggc
     1201 atcagcgtcc tgccattggg cgatgacgcg gtcgcggtcg agatcgacaa tggcttcgat
     1261 ggtgccgccg tcggtgtagt tgtagtaggt gacgatggcg cagttgagtt ggtcgcagcc
     1321 ctggtcttgc cagtagcggg cctcgccggc gctgaggcga accgcgccga cgaactcggt
     1381 cgagcgatcg gccagcagcg ccgccagggt catgtccgcc tgggcgacgg cgcggacggc
     1441 ggccgtgtcc agggcgtagc cggccgggta gccggccacc tcagcgggca tgtcgagcat
     1501 ctgcttgctc ttggccacgg ctaactgaac gctctcgcgg tgcagttggc ggttccagag
     1561 gctgaggaag gcgatgaagg cggcgttgat gagcaggaag aggaggagtc ttcgcgcggt
     1621 gaatgttggt ctcacgggcc aaattatagc acccttgccg aggacgcacg atcaggaaga
     1681 attgaccgcg gcgggcgctg aggacgccgg tttgcgtgga taagaagcct tgccgtgctt
     1741 atcggcgtga gaagccgtgc aatcgacgtc atcatcgcga agccccagcc tgcgattcag
     1801 gagaatcgcg ccacggcttt ggcaaagccc tgatcggtgt gctcatctac tgtccaatct
     1861 gctacactct gccccatgca agctatcact gtcttccagg gccggcgtat cctgctgggg
     1921 gtcaccggtt ccattgccgc ctataagtcc gtcgatctcg cctccaagtt gacgcaggcc
     1981 ggggcgctgg tcgatgtcgt catgactgaa tcggccgctc gctttgtcac gcctctggcc
     2041 tttcgctccg tcaccggccg gcccgtctac accgacatgt ggagcctgga cgaccacgtc
     2101 cgccacgttg gtctgggcga ggcagccgat ctgttcctca ttgccccggc cacggccaac
     2161 acgctggcca agctggccca tgggttggcc gacaacctgc tgacggtgac ggcgctggct
     2221 gcgcgctgtc ccgtcgtcgt cgccccggcg atggacggcg gcatgtacga gcacgccgcc
     2281 acacaggcca acctggctgc cctggcggcc cgcggcgtcg tgctggccgg gccggccgag
     2341 gggcgcatgg cttccgggct gagcggactc ggccggatgc tggagccgga gacgctgctg
     2401 ggggaggcgc gacgcattct ggcgcgcgat ggcgttttga gcggccggcg catcgtcgtc
     2461 accactggcc cgacccgcga ggcgctcgac ccggtgcgct acatcagcaa ccgctccacc
     2521 ggcaagcagg gcgtcgctgt ggctcaggcg gcgctggacg ccggcgcaga ggtgacgctc
     2581 atcgccgggc caattgccgc cagcccgccc actggcgcgc gcgtcattcc cgtggagacg
     2641 acgcagcaaa tgggcgacgc ggtgttgggc tgcgtggccg acgccgacgc gctgtttatg
     2701 gtcgccgctg tggccgactt ccggccggag accatctccg accgcaagat caagaagacc
     2761 gagcggccgg actggggcct ggccatcggc ctggagaaga cgctagacat cctcagcgcg
     2821 gtcaaagagc agcgcgaccg gacaggctat ccgcgcgtcg tcctcggctt cgccgcggaa
     2881 acccacaacg ctttcgagta tggtcgcgat aagctcctgc gcaaggggct gaacttcatt
     2941 gccgtcaacg acgtgaccgc cgctggggcc ggctttgccg tggacaccaa ccaggtggtg
     3001 ctgcttagct cggccggcgt agttgaaacg atgccgttac agagcaaaac ggccatcgcc
     3061 gagcgcctca tccaccatgt tgccgcggcg ctgggctagg cgcgcggacg gcggcgggta
     3121 cgcgcgcggg cggcggccga gcggtcgttg gtcgcggcct ttagtttggc ctccagttcg
     3181 gcccctagct cggcgtccaa cgcggcccgc tcgacgtcgg tcagcggcgt ccgcagcgcc
     3241 tcggcgatgg ccaggatgtg gtcggggccg ttgccgcaac agccaccgat gaagcatgcc
     3301 cccagcgcgc ggacacgccg ggcatagcgg cccattgtgg gtggtgttgc gtcgtagctc
     3361 agttcgttgt gccgccactg ggggatgccc gcgttggcct tggcgatcag gcgcacctcc
     3421 ggcgcttggc ggtgcatggc gtggatgacg ccctcgatct cttcgatgcc gttgccgcaa
     3481 ttgccgccga tggccaccag accccactct ttcatcgtct gcaccgcctg ggccggagta
     3541 acgcccatca tcgtccggcc gttggtgtcg aagctcatcg tcgcagcgat gggtaggtcg
     3601 ctgacggcgc gcgccccctc cacggcggcg cgggcttcct ccaggtcgct catcgtctcc
     3661 acccacagca ggtctacgcc gccggccact aggccgcgag cctgttcggc gaaggcggcc
     3721 cgcgcgtcgt cgaaggtcat gctgcccatt ggcgccagca gttcgcccgt cggccccatc
     3781 gacccggcca cgagaacggg acgaccggcc gcgcccgcct cggcctgcgc gtgctgcgcc
     3841 gcggcgcggt tcaactcgta gacgcggtct tgcaagttgt gcagcttcag ccggtagcgc
     3901 gtgccgccga aggagttggt caggatgacg tctgacccgg cccggatgta gccgcggtgg
     3961 acggcgcgaa tgcgctccgg gtggagtacg ttccatgcct ccggcggcgc gcccgcttgc
     4021 agaccggcgg cgaagagcac cgtgcccatc gcgccatcga gaacgatggt ctcatgggtg
     4081 tcgaggagtt gctgtagctt gtccatagct gcctatgcct gggctttctg ccggcgaatg
     4141 gccatgaatc tcttggctgt ctccacgccg acggccgcgt cgcggcaata ggcgtcggcc
     4201 tcgatctctt cggcgaaggc ctggtttagt ggcgcgccgc cgaccaggac gatgatgtcc
     4261 tggcgaattc cggcgtcctt gagcgcctcg atgacgacgc gcatgtaggg catcgtcgtc
     4321 gtcagcaggg cgctcatgcc caggatgtcg ggcttgtgct tttcaatggc tgccaggaag
     4381 ttgtcgaccg agttattgac gccgagatcg atgatctcga agcccgcgcc ctccagcatc
     4441 atggccgtca agttcttgcc gatgtcgtgg atgtcgccct tgaccgtgcc gacaaccatc
     4501 gtgccgatgc gctccgcgcc ggtctcggcc agcagcggtc gcaggatggc catgcccgcc
     4561 ttcatcgcct tggcggccat gagcacctcc ggcacaaaga ggatgccgtc gcgaaagtcg
     4621 gccccaacga tgtccatgcc ggcgacgaga ccattggtca ggatggcgta aggctcgtga
     4681 ccgcgggcca acattacctg gacctcatcc ttgatctcgt cggcgtagcc gtcgtagagg
     4741 tcttccttca tcagctcata gatttcgtcg tccgacatcg cttgcagatc gatctcttcg
     4801 tcgttcatga cacaagttcc acatcctcat tgtccgggcc gggttgccgg cgccgttgct
     4861 cttctgtcct gcctactcgg ctcagggcgg cccgctcggc gtagggccgc ctacgggcgc
     4921 ggctacaagc gcgcgccgcc cgcccggcgg cgaatccgtt ccgcgcgccg cgctcgccca
     4981 gtgtccccct ggcccgccgc ggcccgcggc aggggcgcgc cgaggcggct ttccagatgg
     5041 gggaagcggt cggcggcgtg gggaatgtgg atggcgctga caaactcgtc ctggaagcgc
     5101 gggtcgatgg ccgtctcgac gtaaaccacc gcgcgggcca gcgccgccgc ctcgtcacgc
     5161 ttgtggcggt tcaggagggc gatgcgcgcc ccatcgccgg cggcgttgcc cacggccacc
     5221 acgcgctcca gtgggcagtc ggggatcaga cccagcagca tggcgtacag tgggtcgatg
     5281 acgctgccaa acgccccggc cagcacaatc cgttccacgc gctcgacgcc catccggttc
     5341 atcagcagct tggctccggc gtagagggcc gccttggcca actggatggc gcgcacgtcg
     5401 tcctgcgtca ccaggatggg ctggccgttg gccgtttggt cgcccgtggc caacacgtag
     5461 gccgccttct tgtcctgcca caacaggcgc gggtgcggcc gatcgtcgct aaagcggccg
     5521 tcgccgcgca gcacgccggc caggtacatc tcggccactg cctcgatgat gccggagccg
     5581 cagatgccgg tggccaggtg gcgcggttgg ctgtccggga gtgcatccac gcccgtgggc
     5641 caggcgtccg accaacgctc ttcgccaata acgcggaagc gggccgtcca atcggctgga
     5701 tcgatacgca cccgctcgat ggctcccggc gcggcgcgca tgccgaagcg gatttgcgct
     5761 ccttcaaagg ccgggccggt ggggctggaa gcgctcagca gccggccgcg attgccgagg
     5821 acgatctcgg catttgtgcc cacgtctacc aacagggtca gttcgtcctg ccggtgcggc
     5881 tcctcggcca gcagcacgcc aacattgtcc gcgccgacgt ggcccgcttc ggccggcaga
     5941 acgtggatgg ccgcgccggg gtggaagcgt agccccagct cgcgggcttt cacgtccacc
     6001 gcgtcgcgtg tggccaacgc gaacggcgcg ccgcccagct cttgcgggtt gatgcccagc
     6061 aggatgtgga tcatggtcgt gttgccgacg aaaacggcgt cgtgaatgtc acgcgtgtgt
     6121 agcccggcgc tgcggacggc acgcacggcc agagcattca gcgcctcgat gatggctgcg
     6181 tgcatcttgt ccagcccgcc ggcgtgggtc atggcatagg cgacgcggct catcaggtct
     6241 tccccgtagg ctacctgcgg gttcatcagt gcttcggtgg ccaaaacggc tccggtacgc
     6301 aggtcgcata gatgaccggc gatggtcgtg ctgccaatat ctacggccag gccgtagatg
     6361 ccctccttgt agccgggctg cacgtcaatc acttcgcgct cgttccagac gaggacggtg
     6421 acggcccagt tgcccgcccg caacgccggc tgaaggcgac gcagagcggg caggtcgatg
     6481 cgcaggtcgc tcaggcccca ctgcgccgcc agcgcggcct gcacgcgccc ccagtcgccg
     6541 cggtgctcgc ccagttcggc gcggtctacc tcgacataga cctggcgtat ggccgggtcg
     6601 atgtcgatgc ggcgggcggt ggccgacttg cggatgatct gcttgtgggc acggctctcc
     6661 tccggcacgg tgataagcag gtcgccggcc acgtgggcgg cgcaggccag gcggccggcc
     6721 gcgccatctg cggaagccag gctggcggcg tcgcccgcgc tggccagcag gtcgcgctcc
     6781 ctggccgcgg ccgggcttag gtgggcggcg ctggagcgga tgccgtgctt ggcaaagtcg
     6841 ccctcctcga cgcggacacg gcacttggcg caggttagcc ggccgccgca gatgctctcg
     6901 atgtctacgc ccatctggcg ggccgcgtcg agcagcgact ggcccgccgg cacccggccg
     6961 cggcggccgg agggcatgaa gatcacctgg aattcgccgt tggccgtggt catatcgcta
     7021 ggtctgggtt gccggggcgc tgcggatccg cctgctgggg cggccggtct gggatggatt
     7081 ctagcatgag agggcggggg ttcaaaagca gccgtagacc tgaccggtgc gcgactccaa
     7141 ccccgtcgct atcacaatac cagcagccgc ctacctgttc ggcctgctcg cgggccatcg
     7201 accactagct gcccttccgt atcgccgcga ccgtcgcctg ccaatcgatg gtcgtgtggt
     7261 gggcgtccag ttcggcggtc accgcggcgg cgacctcgac tggcgagccg tcacgctcct
     7321 cggccgcgct ccaggcgaag ccagacaggt agccatccgt gccggtcagc ccggcggcca
     7381 tggcggcgct atccaccgcg gccgcgaagc gcgccggcag ctcgacgctg acgcggtcgc
     7441 gtcgtccgcc ggcgcgcacc tgaaccggaa tgtcgtgcca gtaaagaatc tggtaagtcg
     7501 tcatgggcag ccaccaaagg gacttgctgt tgccggccgc ctactggcgc gggtcggtcg
     7561 tgtcgccggc tgccggcggg ctgtcggcgc ccggttggcg cgggctgagg ggttggccaa
     7621 gtgccccgcc cggcacctct cgcccgccgc cgcgatagag agggtgggtc gggccgtcgt
     7681 cggccggcga ccaggctccg cgggccagca tgtcgatctt cggttggtgc agggccgtga
     7741 cttggccggt gaagcgggcc agattgaaca ggctgatgtc gtggcgtgtg gcccggcgct
     7801 cggccaggtc ggccataatc tcgccaatga cgctggcgaa cttgtagcca tggccggaga
     7861 agccggcggc aaaggacacc tgcgggtact ccgggtgcag gtcgatgaga aagtggcggt
     7921 cgggcgtgtt ggtgaacagg catgccttga gcatcatcgt cggcccggcc ccgtcgggga
     7981 agtagcgggc agcgaagtcg cgcagcacgt cctcgtcctc gcggtaagcc tcccgtgaca
     8041 gatactcggc ctcgcccgtt tcgccgaagt ggtggtaacg gccgaatttg aagccgggca
     8101 cggcaaagac ggggaagcca tagtagcgcc cctccggcac aagacagttg aacaccggga
     8161 agcgctctgg ccggaaccac tccggccgcg ccggctgcaa ccaggccaac acctgccgct
     8221 ccggcacagc cagcccgtcc aggaatggca gcaagttctg cgcccaggca ccggcggtga
     8281 agacaagagt gtcggccgtg tagcgatcgc ggtcggtgac aacacgcacg gcgtcgcccc
     8341 acggctccca gccgagcagc gcctcgcggc cgtggatttc cgcgcccagc gcgtgggcgg
     8401 cctcgacaaa ggccaccgtg ctccgctccg gcagcagaaa gccgccgtcg ggctggaaga
     8461 cggccaggtt ctcccgcggc agccggtagg cggggtagcg ctcgctgacc tgcgcgctgg
     8521 tcaagatctc gtgcggcagg tcgtactcga tgcacgatcg cagcgagccg gtgaagacgt
     8581 cgctgtcggc cgggccgcag tcgagcgagc cggtgcgata gagcaactgc tccccaccaa
     8641 tgcgctcgat gtccgcccac agctcatagg cgcgccgcag cagggcgacg taggcaatgt
     8701 gctcgtaata ggccaggcgg atgatgcggg tgtagccatg ggaggagccg aggccgtggg
     8761 gaatatcgaa gcgctcaatg cccaggacgc gcttgttgcg ccgggccagt tggtagcagg
     8821 tggcgctgcc catcccgccg acgccgacga caatggcatg gtagtgcttg ctataggccg
     8881 tcatcgtgtt ctccctggtc tgtcgcccgt cgccgggcct gcgctgtcct ggcccggtta
     8941 agcctgaaat gccaggagca caaagagaag aggtgctctt gtctttgtgc tcctggccta
     9001 ctggggcggg tgcgattcgc tcttagaaca ggcccatcac gcggccatcc tcatcgacat
     9061 caacgttcat gtaggccggg cgcgtgccca ggccgggcat cgtccgcatc tcgcccagga
     9121 gcgggtagat gaagcccgcg ccgacggagg cgcgcacttc gcggacagtc agcgtgtagc
     9181 ccgacggcgc gttcttgagc agcgggtcgt ggctgatgct caggtgcgtc ttggccatgc
     9241 agatgggcaa attgccgaag ccgttgtcct cataggactt gatctgcttc tccgcctgcg
     9301 gttcatagat aacttcgccg gcgctgtaga tctcggtggc gatggtctcg atcttggcct
     9361 tgataggcag atcgaggggg tagaggaact ggaagttaga cggcttctcg caggcggcca
     9421 cgacggcctc ggccagcgcc atcgcgccgt cgccgccgtt ggcccaatgg tcggtcatga
     9481 ccgccgtctc cgcgcccgcg gccatggcgg cgcgctccac ggcggcgatc tctgccggtg
     9541 tatcggtgtg gaacttattg acggccacga caacgggcac gccaaagcgc ttggcgttgc
     9601 ggatgtgggc gaccatgttg gccatgccct tctccagcag cgcgacgttc tcgtcggtgt
     9661 aggccttatc cagcggccgg ccggggatca cccgcgggcc gccgccgtgc atcttcaggg
     9721 cgcgaatggt ggccaccagg acaacgcagt tgggtaccag gccggaggtg cgacacttga
     9781 tgtcaaagaa cttctccatg ccgatatccg cgccaaagcc ggactcggtg acaacgtagt
     9841 cgcccagccg cagcgccagc cggtcggcca ggatggacga gttgccatgg gcgatgttgg
     9901 cgaaggggcc ggcatggaca aaggcgggct gcccttccaa cgtctgcaag aggttgggat
     9961 gcagcgcgtc cttcatgagc acggtgaccg cgccgccgac gcccagatcg tccagggtga
    10021 taggcttgcc gtccttgttc gtgccgatga ctgtccggcc ggcgcgctgg cgcaggtcgc
    10081 gcagggcgct tttgtagtcg tcgccgttga ccagagccag aatggccatg atctcgctgg
    10141 cgacggtgat gtcgaagccg gtctggcgcg ggcggccgtc ctcctctggc ccgaggccga
    10201 cgatgatgtt gcgcagggcg cggtcgttga cgtccaagac ccggttccag gtgacgcggt
    10261 aggggtcgat gttcagccgc gacaggccca ccttggccag gcgctcatcg ttcatgcggt
    10321 cttcgtggta ccagcgggcg tcgatggccg cggcgacaag gttgtgggcc gcggtgatgg
    10381 catggatgtc gccggtcagg tgcaggttga agtcctccat ggggatgacc tggctatagc
    10441 cgccgccggc cgcgccgccc ttgatgccga aggttggccc catgctgggc tggcgaatgc
    10501 aggcaatggc ccggtggcca agacgaccga ggccttgcac caggccgacg cttgtcgtcg
    10561 tcttgccttc gcccagcggc gtgggagtga tggccgtcac gtcgatgtac ttgcccatcg
    10621 ggttgttggc cagcttgggc agggtatcca gatggacttt ggctttggtc ttgccgtagt
    10681 agatcaggtc gtcctcggtc aggcctagct gcgcggcgac ctctttgatg ggcaggggcg
    10741 tggcggcttg agcaatatcc aggtcggagg gaacgggatt cagacgcttc gatgcgtggt
    10801 tcatatatct cctcaagaag gcgccggcca tgacggtggg gtgagtggcc gggggttatc
    10861 gacgcaggtc attgtaccgt atttacggga tgaccggcaa acggccacgg cccgtggcca
    10921 gccaacagcg gccgtcggtt cgcggtcata gaagaggcgg ccccgcgcca acgcgcgggg
    10981 ccgccgggca accattgtcc acgccgggag attagacctg cgcaggctgg ccaacggcaa
    11041 agccgcggcg cagcacttgc cggttggcct cgatgagatg ctgctttccg gccggcaaat
    11101 gggcggccag agcggcctcg atgaccgcca acggcaacac cgcccgcgcg gccagcatcg
    11161 cccccacggc ggccatattc agcatcttgg gcgtgcccca ctcctcggcg atgccgttgg
    11221 cgggcacata gacgatgtcg atgtcggtac ggcgcgattc ggaagcgacg agcgagctat
    11281 tgactaccag cagcccaccc ggcttcacca gcggttcgta cttatcaaac gagggcaggt
    11341 ttagcgcgat ggcgatgtct ggctgggcga taacgggcgc gccaatctcg tcgtcgctga
    11401 tgatgaccgt gcaatgcgcc gtgccgccgc gcatttccgg gccataggac ggaatccacg
    11461 agacgaagcg gccggcgtcc atgcctgcgt aggccagcag ttgcccggca aagagcgcgc
    11521 cctggccgcc gaatcccgaa ataatgacag aggtttccat agtctcattt cctcagaaag
    11581 ctggctgcag cgccggaacc aactcgcccg cgtgcgcctg cgccgtgatc gcctgagcaa
    11641 agacctcgcc caggcgctga atacccgtct cgatgcgctg cgggtcgcag agggagaagt
    11701 tgagccgcag ggtgttgtcg ccgccaccct ccgggtggaa ggctccgcca gggacgtagg
    11761 cgacctggtg ggccagggca tccttcagca gtttggccgc gtctagtccc tccggcagcg
    11821 tgacccacag gaacagcccg ccctgcgggt aggtccagtg cacctcgtcc gggaagtagg
    11881 tctctagcgc cgccagcatg acgtcccgtc gctcgcggta gacggcgcga atgcggcgga
    11941 cgtgcttatc caggaagtcg tcctcgccgg tcagaacctc gtaagcgagc atctggttta
    12001 gcgtggccgt gtgcaagtcc acgccctgct tggccacgac acagcgctgc accacctcga
    12061 ccggagccac gagccaggcc aggcgcaagc caggggccag cgtcttggag aaggtgctca
    12121 aatagatgac gttgccgcgc atgtaggccc ggccattgcc gttgtgggcg tcgtcgccat
    12181 agtagacttc gccttgcggg gcgcgcccct tcagccgctc ggcgtcgatg acgaccaggg
    12241 gcggcagatg atcgccttcg tagcgcagtt cgccataggg gtcgtcctcg ataatgggca
    12301 cgccatagtg gtcggcaatg gcgatgagtt gctcgcgcct ctcacgcgac agggtgatgc
    12361 cgcccgggtt ctggaagttc ggcaggatat acatgaactt cggcccggcg cagagggcct
    12421 cttccagtaa gtcaacgcgc aggccgtcgg cgtcggtggg cacggagacg aactcagctt
    12481 gataggcgcg ccaggcttgc agcgcgccca tgtagctggg gcgctcggtc aggatcaggt
    12541 cgcccggatt gatcatgatc ttgccgatca gatcgagcgc ctgttgcgag ccgctggtga
    12601 tcagtacgtt ctccggggca gactcgatgc catagcgggc catttgctgg acgatccact
    12661 cgcgtagcgg ccggtagcct tccgtggggc catactgcaa tgcccggcgg ccctcccgcg
    12721 ccaggacgcg atcggcggcg gcgcgaaagg actccaccgg gaacacctcc ggcgcgggca
    12781 tgccgccggc gaaggagatg atttcggggt tctcgattag cttgagcagc tcgcgaatgg
    12841 tcgaggcgct catccattgc atgcgttggg cgcagcgctg gttccaaggg gttgtcatgt
    12901 caggcttgct cctgtggcgg cgttccggtg ggaacggaac gcctatcggc gcgcgagcgc
    12961 caactccggg gcaactttgt agtcgcccag cggatagacg gggatcatgc tgtcgcgtac
    13021 ccaggccagc gagtcggccg gtgtcatgcc ccagttcgtc gggcaggtcg aaagcaactc
    13081 cacaacgcta aagcccagcc ccttctgctg ggcctcgaag gcggtgcgaa tggccttctt
    13141 ggcctggatg atgtggcggg cgtcgtgaag gctgcgacga acggcgtaag ccgtgccggc
    13201 catctgggcc agtaactcgg ccatgtggat cggcgcgccg gtcagtcgca ggtcgcggcc
    13261 gaagggggag gacgtggtcg tctggcccag cagcgacgtg ggggccatct gcccgccggt
    13321 catgccatag acggcgttgt tgatgaaaat ggtggtgatc cgctcgccac gggcggcgac
    13381 gtgcatgatc tcgcccatgc caatgctggc caggtcgccg tcgccctgat aggtgaagac
    13441 gatcttgtcg ggatggacgc gcttgacgcc agtggccatg gccggggcgc ggccgtgggc
    13501 cgcctcgcaa gagtccacct cgaagtagtt ataggcaaag accgagcagc ccaccgaggc
    13561 gacgaggatg gtatcctcgc ggatgcccag ttcgtcgatg acctcggcca ccaaccgatg
    13621 ggcgatgccg tgggtgcagc cgggacagta gtgggtgtgg acgtcggtca gcgcgtgagg
    13681 gcgctcgtag actatttcct cgcgtcgcgc ctc
//
LOCUS       DAOSMQ010000014        13677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_4515_length_13677_cov_4.969755, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000014 DAOSMQ010000000
VERSION     DAOSMQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4515_length_13677_cov_4.969755"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..705)
                     /locus_tag="PK829_00995"
     CDS             complement(58..705)
                     /locus_tag="PK829_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU39818.1"
                     /translation="MIQAPATNKLDSFSPTMARRMNRLAKLGLDYALTLPTLILIAPL
                     LLVFAVLIRLDSPGPIIYRRRVVGRNGREFDAYKFRTMYVDGNDRLIANRDAWMEVLN
                     GDIDSDPRLTRVGRFLRRYGLDELPRLFNVLNRTMSLVGPRMMTRAELLRFGHRVDGY
                     CSVQPGLTGLWQVSGHSRRVDDRIELESRYVRDWSIGLDLHILLQSVFVAFGATA"
     gene            complement(1133..1630)
                     /locus_tag="PK829_01000"
     CDS             complement(1133..1630)
                     /locus_tag="PK829_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39819.1"
                     /translation="MTFWVLAASYWVHLLSTVVWLGGIALMAFIAWPALRQGTLSANQ
                     WFALQKRFLPWVNAALVLLLITGFVQMTNDENYHGFLAIDSVWAWAMLLKHIAYIGMV
                     VLTATLQFGLYPAMTRLALLAEAQPEMAAAERERLAAREALFLRLNVLCAMTVLLFTA
                     MATAV"
     gene            complement(1714..2892)
                     /locus_tag="PK829_01005"
     CDS             complement(1714..2892)
                     /locus_tag="PK829_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39820.1"
                     /translation="MRRALAVLVLLLLAGCTSLLGPAAPPPTATPPTATPPTATPLPT
                     DLLGDEPVVMSFPSPDYGIHVFMWWKPDTALRDLGLVQAMGFTWVKQKFPWREVEGIE
                     KGQYDWYRTDYIVEQTERAGLKLIVRLDQQPYWSEPADNQWQENGPPGNLADFGDYCG
                     AVAGRYRGRIGAYQVWNEPNLTREWGNRPPDPVAYTALLRVCYTAIKAADPDAIVVSA
                     GLAPTGTNSAEAMPDTDFLQGMYDAGAAAYFDVLGVNAPGYKAPPELSPADAEAPEYG
                     GGRWFAFRHVEDMRALMVANGDGRKQVAILELGWTLDQVNPDYAWHAVDEATQAAYLV
                     RAYQYAAAHWQPWVGLMTTIYIADWEWTPENEQWWWAIVLPDGTPRPAYDALRDMPKE
                     "
     gene            complement(3046..4383)
                     /locus_tag="PK829_01010"
     CDS             complement(3046..4383)
                     /locus_tag="PK829_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU39821.1"
                     /translation="MKPNRKLSLMLIAAFLLLLAACGGGQEAAPEPTVASGVVATLPA
                     ETAATLPPPVVEGEILPTPTLAAPTEGGEVAVPAEQPTTEPAVSGPWPADQFGYGIQV
                     HGNATVGDPAATMQAVRDQLGMNWVKMQVQWWLVEADRDGDQWFFYDAVIEQAHQHGL
                     RLMVSIVGAPAWSRAAGDENGPPDDYNEYARFLSEMIDRHPGQIDAIEVWNEQNLDRE
                     WTSANGISPEDYVRLLQVAHDAIKAKDPNIIVISGALSPTGTGDWIKWADDFDYLDRA
                     LAAGMLNYADCVGAHHNGYNIAPDVTFDAAGNTAEAASAGFRGPFDNPHHSWSFKTTL
                     DTYAQKIQAVDPNKKLCVTEFGWASAEGYSEYPQGFEFALDNTLQEQATYIVQAFQQM
                     HDSGDVWLAFLFNFDFGNKGGGPTDDPVPYSIIDTAGIPRPAFGAVADMPKNP"
     gene            complement(4405..7512)
                     /locus_tag="PK829_01015"
     CDS             complement(4405..7512)
                     /locus_tag="PK829_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU39822.1"
                     /translation="MENNETNPGKRLSWPLIVGFVVVVALILVGLFLPPISLGTRLGL
                     GGDNQPTTADATAAPEQTTSSELPDGVTLTAGSAVGVSRVAAADLATADAALAEAAAT
                     LPADASGDVYVLDYSGDVPTGQIALPLPAGIASPKTIDLFGWDGAGWQHVAGQIDPAG
                     AQAVVANGPLPRALVFVQSAPAAEPVVAAELSPEAALPAEVAPLLGETSVGALVLGEN
                     GSLAGDVAAVPDEATGSARFLRLTNRGAVIDQTALTALLDNAAIQDAQVNSVVGQAKA
                     EGYAGVHLDYQGAPATQSAAYTAFVTKLADALHAEGLQLALTLGLPAAIDSGWDTGGQ
                     DWATLGRLADVVYVQMPLDPTAYGPGEAADQLLAWATGQIDRSKINMLLTAGAVNKVG
                     DAYTELPAEQALADLGTLEFVEGAAEIDPGAPVEVALSGSATPLEWDGEALAYRYTFE
                     QAGQPQTVWLITEAALTDRLRLASEYNLRGATVRGLGLGSGPAYAAVLNSFMGNGDAP
                     QPGGAAIAWTVRDAGDNIVASGTGADLNFAWTTTENPGDYRVQAALVLGDTTAALGEL
                     PVTVGGAAVAEAEAPTATPEATGEATAEATAAPAGAATPAATRPSANLGAPGEATAVV
                     NVPSNVRTGPGISYGTIAGGAQGGTVVELLGRNAAGDWYNISLPGGEEGWISSSLVTV
                     NTGFNTSSLAVVEAPPPAAAVAGAPPASGAAAAPVVVPPSVAPVGNVGNFELGGQAAG
                     LPSGTMQYAGMTWVKKQYKWNPGDPPEAVAGLINEAHASGMKILLSIPGQLAPSSIDF
                     EAYTTFLGGVAALGPDAIEVWNEMNIDREWPAGQIDPTSYVNNMLRPAYQKIKAANPN
                     VMVITGAPAPTGYFGGCGGGGCDDAPYVAGMMAAGAGSASDCIGVHYNEGIMPPSATS
                     GDPRGAGGHYTRYFQGMINAYTGAGAGALCFTELGYLSGEEWGTLPAGFLWRAPYNLT
                     VAEQAQYLAEAVRVGVQSGRVRLIIVFNVDFKTFGDDPQAGYAMIRPNGSCPACETLR
                     QVMGQ"
     gene            7870..8190
                     /locus_tag="PK829_01020"
     CDS             7870..8190
                     /locus_tag="PK829_01020"
                     /inference="COORDINATES: protein motif:HMM:NF026214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOU39823.1"
                     /translation="MSTADLFSTMPSRFQADKATGTNMGILFDLSGPDGGQWFVNIAD
                     GQLTVNEGAPVAMPSATIKMTTADFADLSSGNLNPMMAFMTGKVKVEGDLNSVMKFQQ
                     LVGF"
     gene            complement(8263..10275)
                     /locus_tag="PK829_01025"
     CDS             complement(8263..10275)
                     /locus_tag="PK829_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU39824.1"
                     /translation="MTQMVDILLRGGTVVTMNDRYDVYEDGAVAIRGDSIVAVGPTEE
                     IARDYQAAETVDCADSVIMPGLVNAHTHIPMTLMRGLNDDLRLDVWLGYLMPVEREFV
                     TPAFVKLGARVACAEMIRSGVTSFADMFYYEEAIAEQVAEIGLRALLGQTILVFPAPD
                     AETFEDALLLCRRFIEHWNGHPLIQPAVAPHAWYTATPELLLACADLARAFDVPLHTH
                     VSETKLEADNSVNQNHMSVVTWLEQHGILDTKLLAAHCVHINEEDMFSLRRVGAGVAH
                     CPSSNLKLASGIAPVAQMLKLGLKVGVGTDGPASNNDLDMVEEMRLASFIAKTAAQNP
                     TVLPARQTLEIATIGGARAIHMGHLTGSLEVGKRADVAVRDMRGIHNQPHFHNHPDAV
                     YSRIVYSSKANDVAHVLCNGRWLMRDRVLLTVDEDAAREAAIDVAAKIDAFVIERESS
                     PYNKLVLLAGVQRLESFEVQVKAPVDDDAAVHAALSGGPITVTRAAHYRQYDHYFIFA
                     SGDPDAARLRYREDEFIDESGEVTQVRARLTLIGEGEREELPNAVMLSRSRFLATADR
                     SLRFYQEYFAPARELRVHKDRYRWHILYKATDFAINLDRVLEPELPGYFLEIKARTWS
                     RSDAERKAALITELLGQFGLELDQAERREYTEIALNANTEKEGARG"
     gene            10496..11809
                     /locus_tag="PK829_01030"
     CDS             10496..11809
                     /locus_tag="PK829_01030"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOU39825.1"
                     /translation="MRMVDLIARKRDGGELSSEEIAAFIREYAAGDIPDYQAAALLMA
                     IYLRGMNRRETVDLTLAMAHSGETLDLHDVAPHIVDKHSSGGVGDKTSLVVLPLVAAL
                     GVPVGKMSGRGLGSSGGTLDKMESFAGWSSALSLEQFKRQLADIGLVLAGQTADLAPA
                     DGKLYALRDVTATVSNRSLIAASIMSKKLAAGADAIVLDVKMGSGAFMPTLDAAEDLA
                     RLMVDIGQDAGRRVVALISDMNQPLGHAVGNALEVKEALATLNGGGPADFWEHCRVVA
                     GMMVWLAGGAPTPAAAQALVAAARADGRGLAKFRQLVVAQGGDGRQVDDPARLPQALY
                     VEPVEALAGGYIAAMDTSELGWVCVRCGGGRLVKTDTVDPAVGFLLTVKIGDRVEPGQ
                     VIGEIHADDDMTLQAAQRDIPAALTLSDTPVAAPPLFYKTIGDTG"
     gene            11828..>12505
                     /locus_tag="PK829_01035"
     CDS             11828..>12505
                     /locus_tag="PK829_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU39826.1"
                     /translation="MELLGIDIGGSGIKGAIVNAATGELVTERLRLPAPPEFQPETVI
                     AIVADLVNQFSYSGPIGVGFPAVVMRGEVMTPPTALAFPGWAGVNLAERIHAATGQPT
                     TVGNDADVACLAEMYFGAGKGERGVVMVFTIGTGIGSALFLDGRLVPNFELGRVYLRD
                     SKKTAEQWASDRVRELDGLGWKEWGTRLNAYFQHIELLFWPDVIIIGGGVSKKHEKFF
                     PQIQVRAK"
     assembly_gap    12506..12605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12606..>13677
                     /locus_tag="PK829_01040"
     CDS             <12606..>13677
                     /locus_tag="PK829_01040"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU39827.1"
                     /translation="LCRPPVPTQFQSVELPGGRFIGINVPRSLELHSLSDGRVLVRQR
                     DENRPLSGEEIRALSIARPTVDFETETIPGARRDDLNAETIDEYVEKRETRTGTRGPS
                     PAQLLYEIGATDREGQPTLIGVLLFGRNPQAFVPQSGVVFVKFPGTEPRGEDGGVGYG
                     RRAELTGPLADIIDRAWAIVFDEMRIGATVNGLEREEVLEYPRRAVREALINAVAHRD
                     YRIAGRRIEIRMYADRLEIISPGGLPGYMTLENLVDEHFSRNPRLVNGLFQWGYIEEL
                     GLGIDQMIEEMVQAGHPPPEFRATPHSFTVVLSNKRERAAPPRWTRSMNERQTRAVNF
                     VRESGSITNREYRQLCPDVSPET"
BASE COUNT         2203 a         4361 c         4761 g         2252 t
ORIGIN      
        1 ggccccgccg gagcggggcc ttgccatgac agaaggaggg tgaaacgtga agccagttta
       61 agcggtggct ccgaaggcca cgaagaccga ttgcaacagg atatgcaggt caaggccaat
      121 cgaccagtcg cggacgtagc gcgattccag ttcgatgcgg tcgtcgacgc ggcggctgtg
      181 gccgctgacc tgccacaggc cggtcaggcc gggctggacg ctgcaatagc cgtcgacgcg
      241 atggccgaac cgcagcagct cggcgcgggt catcatgcgc gggccgacga gcgacatggt
      301 gcggttgagg acgttgaaca gccgcggcag ctcgtcgagg ccgtagcggc gcaggaagcg
      361 gccgacgcgg gtcaggcgcg ggtcgctgtc gatgtcgccg ttcagcactt ccatccaggc
      421 atcgcggttg gcgatgagcc ggtcgttgcc gtctacgtac atggtgcgga acttgtaggc
      481 atcgaactcg cgtccgttgc ggccgacgac acgccggcga tagatgatcg ggccgggcga
      541 gtcgagccgg atgaggacgg cgaagaccag cagcagcggg gcaatcagga tgagcgtcgg
      601 cagagtcagc gcgtagtcca ggcccagctt ggccaggcgg ttcatgcgcc gtgccatcgt
      661 cggggagaag gaatcgagtt tattggtagc tggagcctga atcatgtttt tatcccaggc
      721 gcatctgcgg ggcagcgctg atgagcctag agatagtcta ggctggctcg cttacgaaaa
      781 aacttacgaa aggccacttg tattaatcat tatcctgctg gcgctggcgc tagttggcag
      841 cgcggcggcg ctgggcgaaa gccagccggc cgcgaacacc aggggcgcaa gcgcccgaac
      901 tggtcgttgt aacggattaa gttgtagggc gggatggcat cccgccgtct tcggttgtca
      961 gattatgctg ctgaagttct ggggagaaga ggcgggctgc cagcccgcgc tacatggtcg
     1021 tcggacgaag aggcgggctg ccagcccgcg ctacatggtc gtcggacgaa gaggcgggtt
     1081 gccaacccgc gctacatggt cgtcggacga agaggcgggt tgccaacccg cgctacacgg
     1141 ctgtggccat ggccgtgaag agcagcacgg tcatggcgca gaggacgttg aggcgcagga
     1201 agagcgcttc gcgggcggcc agacgctcgc gctcggccgc ggccatctcc ggctgcgcct
     1261 cggccagcag cgccagccgg gtcatggccg ggtagaggcc gaattgcagc gtggccgtca
     1321 ggacgaccat gccgatgtag gcgatgtgct tcagcagcat ggcccaggcc cagacgctgt
     1381 cgatggccag gaagccgtgg tagttctcat cgttggtcat ctggacgaag ccggtgatga
     1441 gcaggaggac aagggcggcg ttgacccagg gcaggaagcg cttttgcagg gcgaaccatt
     1501 ggttggccga tagcgtgccc tggcgcagtg ccggccaggc gatgaaggcc atcagggcga
     1561 tgccgcccag ccagacgact gtcgagagca ggtgaaccca gtaggatgcg gccagaaccc
     1621 agaatgtcat gtgtctcctc caagcaaggt tccaggttcc aggggccagg ttccagggaa
     1681 gagcgcttct ccctggaacc tggcccctgg aacctactct ttcggcatgt ccctcagcgc
     1741 atcatacgcc gggcgcggcg tgccgtcggg caagacgatg gcccaccacc attgctcatt
     1801 ctccggcgtc cattcccaat cggcgatgta gatcgtcgtc atcaggccga cccacggttg
     1861 ccagtgcgcg gcggcatact gataggcgcg cacgaggtag gcggcctgcg tcgcctcgtc
     1921 cacggcgtgc caggcgtagt cggggttgac ctgatcgagc gtccagccca gctccaggat
     1981 ggccacctgc ttgcggccgt cgccgttggc caccatcagg gcgcgcatgt cctcgacgtg
     2041 gcggaaggcg aaccagcgcc cgccgccata ctccggggcc tcggcgtcgg ccgggcttag
     2101 ctccggcgga gccttgtagc cgggggcatt gacgcccagc acgtcgaagt aggccgccgc
     2161 gccggcatcg tacataccct gcagaaagtc ggtgtcgggc atggcctcgg cgctgttggt
     2221 gcccgtgggg gccagcccgg cggagacgac gatggcgtcg gggtcggcgg ccttgatggc
     2281 ggtgtagcag acccgcaaca gggcggtgta ggccaccggg tcgggcgggc ggttgcccca
     2341 ctcgcgggtc aggttcggct cgttccagac ctggtatgcg ccgatgcgcc cgcgatagcg
     2401 cccggccacc gcgccgcagt agtcgccgaa gtcggccagg ttgccgggcg ggccgttctc
     2461 ctgccactgg ttgtcggccg gctccgacca gtagggctgc tggtcgaggc gaacgatgag
     2521 cttcagcccg gcccgctccg tctgctcgac gatgtagtcg gtgcgatacc agtcgtactg
     2581 gcccttctcg atgccctcca cctcgcgcca ggggaacttc tgcttgaccc aggtgaagcc
     2641 catggcctgc accagcccca ggtcgcgcag ggctgtgtcc ggcttccacc acatgaagac
     2701 gtggatgccg tagtcgggcg aggggaagga catgaccacc ggctcgtcgc ccagcaggtc
     2761 ggtgggcagg ggcgtggccg tgggcggcgt ggctgtcggc ggcgtagctg ttggcggcgg
     2821 cgccgccggc cccaacagcg acgtgcagcc ggccagcaac aggagaacga ggacagcgag
     2881 ggcgcggcgc atgggggagt gttcagtatc cagtatccag tatccagtat tcagtattca
     2941 gtgggtcagt aggacagtgc gtcaatggca ggggccaacg aacaagccac tgacctactg
     3001 acctactgac ctactgttta ctggccactg cccactgcct actgcttacg gattcttggg
     3061 catgtctgct accgcgccga aggcggggcg ggggatgccg gccgtgtcga tgatgctata
     3121 gggcacgggg tcgtcggtcg ggccgccgcc cttgttgcca aagtcgaagt tgaacaggaa
     3181 ggccagccag acatcgccgg agtcgtgcat ctgctggaac gcctggacga tataggtggc
     3241 ctgctcctgc agggtgttgt cgagggcgaa ctcaaagccc tgcggatatt cgctatagcc
     3301 ttcggcgctg gcccaaccga actccgtcac acacagcttc ttattcgggt caacggcctg
     3361 aatcttctgg gcgtaggtgt cgagcgtggt cttgaagctc caggagtggt gggggttgtc
     3421 gaacgggccg cggaagccgg ccgacgctgc ctcggcggtg ttgccggcgg cgtcgaaagt
     3481 cacgtcgggg gcgatgttgt agccgttgtg gtgcgcgcca acgcagtcgg cgtagttgag
     3541 catcccggcg gccagagcgc ggtcgaggta gtcgaagtca tcggcccact tgatccagtc
     3601 gcctgtgccc gtgggcgaga gcgcgccgct gatgacgatg atgttggggt ctttggcctt
     3661 gatggcgtcg tgggcgacct gcaggaggcg cacgtagtct tccgggctga tgccgttggc
     3721 gctggtccac tcgcggtcga ggttctgctc gttccagacc tcaatggcgt cgatctgccc
     3781 cgggtggcgg tcgatcatct cgctcaggaa gcgggcatac tcattgtagt cgtcgggtgg
     3841 gccgttctcg tcgccggcgg cgcggctcca ggccggcgcg ccgacgatgc tgaccatcag
     3901 gcgcagcccg tgctggtggg cctgctcaat gacggcgtcg tagaagaacc actgatcgcc
     3961 gtcgcggtcg gcctccacca accaccactg cacctgcatc ttgacccagt tcatgcccaa
     4021 ctggtcgcgg acggcctgca tggtcgccgc cgggtcgcca acggtggcgt tgccgtggac
     4081 ctggatgccg tagccgaact ggtcggccgg ccaggggccg ctgacggccg gctctgtcgt
     4141 cggctgctcg gcgggcacgg cgacttcgcc gccctcggtt ggcgcggcca gcgtcggcgt
     4201 gggcaggatt tcgccttcga cgacgggtgg tggcagcgtg gccgccgtct cagccggcag
     4261 cgtggccacc acgccgctgg cgacggttgg ctctggggcg gcctcctgcc ccccgccaca
     4321 ggcggcgagg agcaacagga aagcggcaat gagcatcagg ctcagcttgc gattcggctt
     4381 cattcgggtt ttctcctcct gcgcttattg gcccatgacc tgccgcagcg tttcgcacgc
     4441 cgggcagcta ccattgggcc ggatcatggc gtagccagcc tgcgggtcgt cgccgaacgt
     4501 cttgaagtcg acattgaaga cgatgatcag gcgcacccgg ccgctctgca cgccaacgcg
     4561 cacggcctcg gccaggtatt gcgcctgttc agccacagtc agattataag gggccctcca
     4621 gaggaaacca gccggcagcg tgccccattc ctcgccgctg agataaccca gctcggtgaa
     4681 gcacagcgcg ccggcgccag cgccggtgta ggcattgatc atgccctgga agtagcgggt
     4741 gtagtgaccg cccgcgccgc gcgggtcgcc gctggtggcc gacggcggca tgatgccctc
     4801 gttgtagtgg acgccaatgc agtcggaggc gctgcccgcg ccggcggcca tcatgccggc
     4861 cacgtagggc gcgtcgtcgc aaccaccacc gccgcagccg ccgaagtagc cggtcggggc
     4921 cggcgcgccg gtgatgacca tgacgttggg gttggccgcc ttgatcttct ggtaggccgg
     4981 ccgcagcatg ttgttgacgt atgaagtggg gtcgatctgg ccggcgggcc actcacggtc
     5041 gatgttcatc tcgttccaga cctcaatggc gtctggcccc agcgcggcca cgccgcccag
     5101 gaaagtcgtg taagcctcga agtcgatgct gctgggggcc aactggccgg ggatgcttaa
     5161 caggattttc atacctgagg cgtgggcctc gttgatgaga ccggccacgg cttcgggcgg
     5221 gtcgccgggg ttccacttgt actgcttctt gacccaggtc atgccggcgt attgcatggt
     5281 gccggagggc aggcccgcag cctggccgcc caactcgaag ttaccgacgt tgccgaccgg
     5341 cgcgaccgag ggcggcacga ccacaggcgc ggccgccgcg ccgctggccg ggggcgcgcc
     5401 cgcaaccgcc gctgcggggg gcggcgcttc gacgaccgcc aggctgctgg tgttgaagcc
     5461 ggtgttgacc gttaccaggc tgctggaaat ccagccctct tcgccgccgg gcaggctgat
     5521 gttataccag tcgccggcag cgttgcggcc gagcagttcg acgaccgtgc cgccctgcgc
     5581 cccgccggcg atagtcccat aggagatgcc ggggccggtg cggacgttgg agggcacgtt
     5641 gacgacggcg gtggcctcgc ccggcgcgcc gaggttggcc gacgggcggg tggccgcggg
     5701 cgtggccgct ccggccggcg cagcggtggc ttcggccgtg gcctcccccg tcgcttccgg
     5761 cgtggctgtc ggcgcttcgg cctcagccac ggccgccccg ccgacagtca ccggcagttc
     5821 gcccagagcg gcggtcgtgt cgcccagcac cagcgcggcc tggacgcggt aatcgccggg
     5881 attctcggtc gtcgtccagg caaaattcag gtcggcgccc gtgccgctgg cgacgatgtt
     5941 gtcgccggca tcgcgcacgg tccaggcgat ggccgcgccg ccgggttggg gcgcgtcgcc
     6001 attgcccatg aagctattga gcacggcggc ataggccggg ccgctgccca accccagccc
     6061 gcgcaccgtc gcgccgcgca ggttatactc gctggccagc cgcagccggt cggtcagcgc
     6121 cgcctcggta atgagccaga cggtctgcgg ctggccggcc tgctcgaagg tgtagcggta
     6181 ggccagggcc tcgccgtccc attccagcgg cgtggccgag ccggatagcg ccacctcgac
     6241 cggcgcgccg gggtcgatct cggccgcgcc ttcgacaaac tccagcgtgc ccaggtcggc
     6301 cagagcctgc tcggccggca gttcggtgta ggcatcgcca accttgttga ccgcgccggc
     6361 ggtcaacagc atgttgatct tgctccggtc gatttgccca gtggcccagg ccagaagctg
     6421 gtcggcggcc tcgcccggcc cataggcggt cgggtcgagg ggcatctgga catagacgac
     6481 atcggccagg cggccgagcg tggcccagtc ctggccgccg gtgtcccagc cgctgtcaat
     6541 ggcggccggc aagcccagcg tcagagccag ttgcagtccc tcggcgtgga gcgcgtcggc
     6601 cagcttggtg acgaaggcgg tataggccgc cgactgtgtg gccggcgcgc cctggtagtc
     6661 gagatggaca ccggcatagc cctcggcctt ggcctggccg acgacgctgt tgacctgggc
     6721 gtcctggatg gcggcgttgt cgagcagggc agtcagggcg gtctggtcga tgaccgcgcc
     6781 gcggttggtc aggcgcagga agcgggcgct gccggtggcc tcgtccggca cggcggctac
     6841 atcgccggcc agcgagccgt tctcgcccag caccagcgcg ccgacggagg tctcgcccaa
     6901 cagcggggcg acctcggccg gcaaggcggc ctcgggcgac aactcggcgg ccacaaccgg
     6961 ctcggccgcc ggggccgact ggacgaacac cagcgcccgc ggcagcggcc cattggcgac
     7021 cacggcctgc gcgccggcgg ggtcgatctg cccagcgacg tgctgccagc ccgcgccgtc
     7081 ccagccgaaa aggtcgatgg tcttgggcga ggcgatgccg gctggcaacg gcagggcgat
     7141 ctgcccggtg gggacgtcgc cgctatagtc gaggacgtag acatcgccgc tggcgtcggc
     7201 gggcagcgtt gccgcggctt cggccagcgc ggcgtcggcc gtggccaggt cggccgcggc
     7261 cacgcgactg acgccgacag cgctgccggc ggtcagcgtc acgccgtcgg gcagctcgct
     7321 gctagtcgtc tgttccggcg ctgcggtggc gtccgcggtt gtcggctggt tatcgccgcc
     7381 cagccccagg cgcgtaccca gcgagatcgg tggcaggaaa agccccacca gaatgagggc
     7441 aacgaccacc acaaagccga caatcagcgg ccaagacagg cgcttgcccg gattggtttc
     7501 attgttttcc atgaagttaa gccccttccc ggaagatgtt gaatttgtga ttcaagtttg
     7561 ggaatacacg acgaaaagcg gcatcatctg gccgcccgtc tcttcctctc cggtaaacta
     7621 gcgcgcattc tagcacaagc gccccgctcg gcaacagccc ccgcggtctt atcgacctat
     7681 cgtcgggctg tcgttgagga aacattaacc aagcgggcgc tgcttgacag aggcaacccc
     7741 cctgttatac tcgccttcga agccccgcgt cgcgggaaaa gagcacacaa ggcgggtaaa
     7801 ccacgaccct tctcgtcgct accagcccgg taggggatag ccaacagccc ttaaggagac
     7861 gaccgaaaga tgagcacagc cgatctattt agcaccatgc cctcccgctt ccaggcggac
     7921 aaggccaccg gaaccaacat gggtatcctg tttgatctga gcggcccgga tggtgggcag
     7981 tggttcgtca atatcgccga tggacaactg accgtgaacg agggcgcgcc ggtggccatg
     8041 cccagcgcga ccatcaagat gaccactgcg gacttcgctg acctgtctag cggcaacctg
     8101 aacccgatga tggccttcat gaccggcaag gtcaaagtcg agggcgacct gaactcggtc
     8161 atgaagttcc agcaactggt cggcttctag gttatagggg ccaggggcta ggggccaggg
     8221 attaggttcc agggaagaac gctcttccct agcccctacc ccctaacctc tggccccttc
     8281 tttctccgtg ttggcgttca gggcgatctc ggtgtactcg cggcgctcgg cctgatctag
     8341 ctccagtccg aactgcccca gcaactcagt gatcagcgcc gccttccgct cggcatcgct
     8401 gcgcgaccag gtgcgggcct tgatctccag gaagtagccc ggcaactccg gttccagcac
     8461 gcggtcgagg ttaatggcga agtcggtggc tttgtagagg atgtgccagc ggtagcggtc
     8521 cttgtggacg cgcagctcgc gcgccggggc gaagtactcc tggtagaagc gcaggctgcg
     8581 gtcggccgtg gccaggaagc gcgagcgcga gagcatgacc gcattgggca actcctcgcg
     8641 ttctccctcg ccgatgagcg tcagacgggc gcgcacctgc gtcacctccc ccgattcatc
     8701 gatgaattcg tcttcgcgat agcgcagccg ggccgcgtcc gggtcgccgc tggcgaaaat
     8761 gaagtagtgg tcatattggc ggtagtgggc ggcgcgcgtc accgtgatcg gcccgcccga
     8821 cagggccgcg tggacggccg cgtcgtcgtc caccggcgct ttcacctgta cctcgaagct
     8881 ctccagccgt tggacaccgg ccaatagcac cagtttgttg taagggctgg actcgcgctc
     8941 gatgacaaag gcgtcgattt tggccgccac gtcgatggct gcctcgcgcg cggcatcctc
     9001 atcgacggtc agcagcacgc ggtcgcgcat cagccagcgg ccgttgcaga gcacgtgggc
     9061 cacgtcgttg gccttgctgg aatagacaat gcgcgagtag accgcgtcgg ggtggttgtg
     9121 gaagtggggc tgattgtgga tgccgcgcat gtcgcgcacg gcgacgtcgg cgcgcttacc
     9181 tacctccagg ctgccggtga gatggcccat gtgaatggcc cgcgccccgc cgatggtggc
     9241 gatctccagc gtctggcggg cgggcagcac cgtcgggttc tgcgcggccg tcttggcaat
     9301 aaacgacgcc agccgcattt cctcgaccat gtccaggtcg ttgttgctgg ctgggccgtc
     9361 tgtgcccacg ccgaccttca ggccgagctt gagcatctgg gccacggggg cgatgccgct
     9421 ggccagcttg aggttgctcg acgggcagtg ggccacgccc gcgccgacgc gccgcaggct
     9481 gaacatatcc tcttcgttga tgtggacgca gtgggcggcc agcagcttgg tgtccaggat
     9541 gccgtgttgc tccagccagg tgacgacaga catgtggttc tggttgacgc tgttgtcggc
     9601 ctccagcttg gtctcgctga cgtgggtatg caggggcacg tcgaaggcgc gggccaggtc
     9661 ggcacaggcc agcagcagct ccggcgtggc cgtataccag gcgtgggggg cgacggccgg
     9721 ctggatgagc gggtggccgt tccaatgctc gatgaagcgc cggcagagca gcagcgcgtc
     9781 ctcgaacgtc tcggcgtcgg gcgcggggaa gacgagaatg gtttgcccca gcagggcgcg
     9841 caggccgatc tccgccacct gctcggcgat ggcctcttca taatagaaca tgtcggcgaa
     9901 ggaggtgacg ccggagcgaa tcatctcggc gcaggccacg cgcgcgccca gcttgacgaa
     9961 ggcgggcgtg acgaactcgc gctcgacggg catcaaatag cccagccaga cgtccaggcg
    10021 caggtcgtcg ttcaggccac gcatcagggt catggggatg tgggtgtggg cgttgaccag
    10081 gccaggcatg atgacgctgt cggcgcaatc gaccgtctcc gcggcctgat agtcgcgggc
    10141 gatctcctcc gtcggcccaa cggcgacgat gctgtcgccg cgaatggcca ctgcgccgtc
    10201 ttcgtagacg tcgtacctgt cgttcatggt cacgacggtg ccgccacgca aaagaatgtc
    10261 taccatttgg gtcataggta tctcctgaga aaggaaccgc gccggtttca atggtggcgg
    10321 gcggtgcctg tgtcgattcc gtggttaagg atgtcggaag ccgccggcgc ggcacaaccg
    10381 cattataccg attcctgata taattcataa actagacagc cgggcagtga agtgggtggg
    10441 tggggaacgg gctaccgatg ggcggcccac aagtgacgcg attggagcaa ttcgtatgcg
    10501 catggtcgac ctgatcgccc gcaaacggga tgggggcgag ttatcgagcg aggagatcgc
    10561 cgctttcatc cgcgaatatg ccgccgggga cattcccgac taccaggccg cggccctgct
    10621 gatggccatc tatctgcgcg gcatgaaccg gcgcgagacg gtcgatctga cgctggccat
    10681 ggcccactcc ggcgagacgc ttgacctcca cgacgtggcc ccgcatatcg tcgacaagca
    10741 ctcctccggc ggcgtgggcg acaagaccag cctggtggtg ctgccgctgg tggccgcgct
    10801 gggcgtgccc gtgggcaaga tgagcggccg cggcctgggc agcagcggcg gcacgctcga
    10861 caagatggag tcgttcgccg gctggtcgtc ggccctgtcg ctggaacagt tcaagcgcca
    10921 gttggccgac atcggcctgg tgctggccgg gcagacggcc gacctggccc cggccgacgg
    10981 caagctctac gccctgcgcg acgtgacggc cacggtcagc aaccgttcgc tcatcgccgc
    11041 gtcgatcatg tccaagaagc tggccgccgg ggccgatgcc atcgtgctcg acgtgaagat
    11101 gggcagcggc gcgttcatgc ccacgctgga cgcggccgaa gacctggccc gactcatggt
    11161 cgacatcggc caggacgccg ggcggcgcgt cgtggccctc atctccgaca tgaatcaacc
    11221 tctgggtcat gccgtgggca acgcgctgga ggttaaagag gccctggcga cgctgaacgg
    11281 cggcgggccg gccgacttct gggagcattg ccgcgtcgtg gccggcatga tggtctggct
    11341 ggccgggggc gcgcccacgc cggccgcggc gcaggcgctg gtggccgcgg cgcgggccga
    11401 cggccgcggc ctggccaagt ttcgccaact ggtcgtggcc cagggtggcg acgggcgaca
    11461 ggtggacgac cccgcgcggc tgccccaggc gctctacgtc gaaccggtcg aggccctcgc
    11521 cggcggctac atcgccgcga tggacacgtc tgaactgggc tgggtgtgcg tgcgctgtgg
    11581 cggcggccgg ctggtgaaga ccgatacggt ggatccggcc gtcggcttcc tgctgacggt
    11641 caagatcggc gaccgggtcg agccgggcca ggtcatcggc gaaatccacg ctgacgacga
    11701 catgaccctg caagcggcgc aacgcgacat tccggcggcg ctgacgctga gcgatacgcc
    11761 ggtggcggcg ccgccgctgt tctataagac aattggcgac acgggctaga gccacgggga
    11821 aaacgacatg gaactgctgg gcatcgatat tggtgggtcg ggcatcaagg gagcgatcgt
    11881 caacgcggcc acgggcgaac tggtgacgga acggctgcgc ctgcccgcgc cgccagagtt
    11941 ccagccagag acggtcatcg ccatcgtggc cgatctggtc aaccagttca gctacagcgg
    12001 ccccatcggc gttggcttcc cggccgtggt catgcgcggc gaggtgatga ccccgccgac
    12061 ggcgctggct ttccccggct gggcgggcgt caacctggcc gagcgcatcc acgcggccac
    12121 cggccagccg acgaccgtgg gcaacgatgc cgacgtggcc tgcctggccg agatgtactt
    12181 tggcgcgggc aagggcgagc gcggcgtggt catggtcttc accatcggca cgggcatcgg
    12241 cagcgccctg ttcctcgacg gtcgtcttgt gcccaacttc gagttggggc gcgtctacct
    12301 gcgcgacagc aagaaaacgg ccgagcaatg ggcctccgac cgcgtgcgtg agttggacgg
    12361 cctgggctgg aaggagtggg gcacgcggct gaacgcctac ttccagcaca tcgagttgct
    12421 cttctggccc gatgtcatca tcatcggcgg cggcgtcagc aagaagcacg agaagttctt
    12481 tccccagatt caggtgcggg ccaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnccctg tgccggccgc ctgtgccgac gcaattccag tcggttgagc tgcccggcgg
    12661 gcgcttcatc ggcatcaacg tgccccgcag cctggagctg cacagcctgt ccgacggccg
    12721 cgttctggtg cgccagcgcg acgaaaaccg ccccctcagc ggcgaggaga ttcgcgccct
    12781 cagcatcgcc cggccgacgg tcgatttcga gacagagact atccccggcg cgcgccgcga
    12841 cgacctcaac gcggaaacga tcgatgagta cgtggagaag cgcgagacgc gcacgggcac
    12901 gcgcggcccg tcaccggcgc aactgctcta cgagatcggc gcgaccgacc gcgaggggca
    12961 gccgacgctc attggcgtgc tgctcttcgg ccgcaacccg caggccttcg tacctcagag
    13021 cggcgtcgtc ttcgtcaagt tccccggcac cgagccgcgc ggcgaggatg gcggcgtggg
    13081 ctatggccgg cgcgccgaac tgaccgggcc actggccgac atcatcgacc gcgcctgggc
    13141 catcgtcttc gacgagatgc gcatcggcgc gacggtcaat gggctggagc gcgaggaagt
    13201 gctggaatac ccgcggcggg cggtgcgcga ggcgctcatc aacgccgtcg cccaccgcga
    13261 ctaccgcatc gccggccggc gcatcgaaat ccgcatgtac gccgaccggc tggagatcat
    13321 cagccccggc ggcctgcccg gctatatgac cctggagaat ctcgtcgacg agcacttctc
    13381 gcgcaaccca cggctggtca atggtctgtt ccagtggggc tacattgagg agttggggct
    13441 gggcatcgac cagatgattg aggagatggt gcaggccggc cacccgccgc cggaattccg
    13501 ggccacgccc cactccttta ccgtcgtcct gtcgaacaag cgcgaacggg cggccccacc
    13561 gcgctggacg cgcagcatga acgagcggca gacgcgggcg gtcaacttcg tgcgcgaatc
    13621 gggcagcatc accaaccgcg agtatcgcca gctttgcccc gacgtgtcgc cggagac
//
LOCUS       DAOSMQ010000015        13387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_4638_length_13387_cov_4.982148, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000015 DAOSMQ010000000
VERSION     DAOSMQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4638_length_13387_cov_4.982148"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK829_01045"
     CDS             <1..503
                     /locus_tag="PK829_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU39828.1"
                     /translation="IMGISSPGRVYDNIRYGRLEASREEIEAAARAANAHDFIISDLS
                     EGYHTAVGEHGVKLSGGQRQRIAIARAILKDPRILILDEATSSLDSASESLVQEALER
                     LMVGRTSFVIAHRLSTVMNADWILVMNRGELVEQGTHASLLLNPDGLYAQLHRMQFAE
                     NRPAAV"
     gene            complement(645..812)
                     /locus_tag="PK829_01050"
     CDS             complement(645..812)
                     /locus_tag="PK829_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39829.1"
                     /translation="MMWLLFFLGASVFSSIFVLAAGIMSSRMNRHDNYVETFITDTRE
                     APSDNTARTRG"
     gene            complement(1002..1778)
                     /locus_tag="PK829_01055"
     CDS             complement(1002..1778)
                     /locus_tag="PK829_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020107883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOU39830.1"
                     /translation="MTTYENILTRVEGRAGVAQLDRPKALNALNRELMTELMTALEAF
                     DADPAVGCMVVTGSDRAFAAGADIKAMAAATPAQMMAGSFIDLWDRLHHIGKPVIAAV
                     SGFALGGGCELAMACDLIIASETAQFGQPEINLGVIPGAGGTQRLTLAVGKAVAMEMV
                     LNGRYLSAEEARRAGLINHVYPVEVYLEEAVRLANEIAARAPVAVRLAKEAINATFEM
                     SLRAGLDHERRLFYLLFSTEDQKEGMDAFINKRRAEWHGR"
     gene            complement(1852..2670)
                     /locus_tag="PK829_01060"
     CDS             complement(1852..2670)
                     /locus_tag="PK829_01060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU39831.1"
                     /translation="MPRKTLGATSPPLPAREPVAPIVGRNRVLLAILLPLAAALLLGI
                     ITGALFQRGEVASASAAPLFAALGLASWFLGLKLYGLRGMALRGGRPLFAGIGFAVLA
                     WVVALIGRYMLVEIVAIRSAGSGRAFAYLLLFEAFAVQLWAFGLVFRALADYRGGLTA
                     AIVSGLLFGLVGFTLFGESFVAGMDVSVLAGLTSLLYFLLWGVLYGIIRLRTGSILGT
                     ILVQALQTFTVWFVFQPPDDLKIAGLQTCFLATGLLFLIIIWRLWPRQETDYRI"
     gene            2718..3962
                     /locus_tag="PK829_01065"
     CDS             2718..3962
                     /locus_tag="PK829_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU39832.1"
                     /translation="MNHSPDSSPAAPPADLSALPAADWGWDARRAIQLGILTFARFCL
                     NTMLRVVYPFAPAFARGLGVPVTTIYLLISLRNFTGLLSPVFAPLAERYGRRNVMSLS
                     MAVFSAAALLVAGVPAVWTLGAALVVVGAVKVIYDPAMQSYLGDAVPYQQRGRALAIT
                     EFGWSGAFLIGAPLTGWLIGRQGWPAPFLWLGIGGAVGTVLLWRTLPAARRHAHGTIT
                     IGYTWQMLRRHPIIWAAALYMLLPLIAQEALFIVYGDWMESTFNLSLTGLGLATTLIG
                     LAEMSGEATAGWSVDRFGKRPVVALSGVLTAGVFLLIPLSAGSLTTALAALVALFFLF
                     EIAVVGAIPLLTELVPEARGVVMSMSFGAMAAGRTIGSIIGPAVWLRVGMWGNSLIAA
                     VVMLLAVVVLARWLREANPGGA"
     gene            3962..5251
                     /gene="hutI"
                     /locus_tag="PK829_01070"
     CDS             3962..5251
                     /gene="hutI"
                     /locus_tag="PK829_01070"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOU39833.1"
                     /translation="MEIVDLLVYSAGQVCVVPPHDGPQRGAALGDLGLLAGGAVAVDG
                     GRIVAVGPTAELQARFRAEAMIDAGGRCVVPGFVDPHTHLPWQGDRATEFEQRLAGAS
                     YMEIMAAGGGIMSTVRRTRAASVADLVANNQPRLARMLRHGTTSAETKTGYGLETAAE
                     LRQLDAIMALSAAQPVELTPTFLPAHAVPAEFAGDTDGYVDYLVDDTLPAGAAWMRAR
                     GVVLFCDVFCEAGVFDLAQTRRILEAAAALGYRLKVHADEFVGLGGTRLAVELGAVSA
                     DHLVSTPAEDIAALGRGDTIAVGLPGTPFGLAHRDYTPAKAILAAGGALALATDCNPG
                     TCWCESMQMVIALACRYMGLTQAQALAAATLNAAYAIGRGREVGSLTPGYAADLLILD
                     VADYRQLGYRFGTNLVQTVIKRGRVVAVNGLEGEWTE"
     gene            5239..5418
                     /locus_tag="PK829_01075"
     CDS             5239..5418
                     /locus_tag="PK829_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39834.1"
                     /translation="MDGVNLTTAGWIMLAVIVLALLAVAVRYFISVAMEFTRVLLVAG
                     GVLLVAILFWILFVH"
     gene            5614..6558
                     /locus_tag="PK829_01080"
     CDS             5614..6558
                     /locus_tag="PK829_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOU39835.1"
                     /translation="MNYRIVTLLALLLLLVAGCRQNPAATGETAPATPAITATRPPPV
                     ASTSTPVPPTPTPTEPLAALVNGEDITLAAFERELARFTAAQPDAPAADAAQQVLDAL
                     IERQLIVQAAAAEGVAVAPEAVEAKLAELRATYATPAEFDTWLQTNGYTEDEFRAALA
                     AEMTVGEMTARVTAGVPTTAEQVRARYIQMDDATLAQSVLDRARAGDDFAFLAEQNSV
                     DRVTAEIGGDLGFFARGSLLVPEVEAAAFALQPEEISDVIAVTGADGKTIYYIIQVTE
                     RDPERELTADATAALLQAAFSDWLDGLRAAATIERFVP"
     gene            complement(6555..7562)
                     /locus_tag="PK829_01085"
     CDS             complement(6555..7562)
                     /locus_tag="PK829_01085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU39836.1"
                     /translation="MTQFAISKTRWNWKLPTYGQNWTEMIDVAIIIVSWNVRNYLSDC
                     LRSVAADLRASRLRGEIWVIDNASTDGTVALLEDLFPQVHVIANQTNVGFGAANNQGM
                     RAAAASRPRYYLFLNPDTLVRPGAIGRMAKCLDDRPKGGMASARLIFGDGRFQHSAFS
                     FPGLGQLVFDLYAMPPRLYDSRLNGRYPRRLFQPNRQPFAVDHPLGATMLVRADVAES
                     TQGFDESFHMYCEEIDWSWRVRESGWEIYVVPEAEIVHYGGESSRQIPAQSMLNLWES
                     RAQLYRKHHGPLKQRVAGWLVRRKMARRARLAQDPQLKNAYERIVLAWSADAAGRKGL
                     A"
     gene            complement(7492..8706)
                     /locus_tag="PK829_01090"
     CDS             complement(7492..8706)
                     /locus_tag="PK829_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOU39837.1"
                     /translation="MKRHHITITLSASLLAAIAFLPSCRPEAVTVEVTRLVVAAPSET
                     AAPPSPTTALAGAATSAPTVAAPPVPVEVTVEVTRPPLGTAERPVQLLFLPTTTAAVI
                     AQRTEPLVAALEAATGAEFAVGIPDNEAAVAALLCAAPADVIAFVSAAAYTVAHDECG
                     AVAGLVARHDDGLTWQMGMLVTRPGAAGALDDLDGRRWAVADTRSLPNYLYFRAQMAE
                     AGIEPGEVVTTPEDTSALLALRDQEVDFTTADFVPPIMPLERQWIYGETDPEEWRVLG
                     IAPTRSPIGYVLVAGEPELGGYRLRDARARLFDTTPDIFDVTRILALSEPIPNETVVF
                     GADFPLSLARQTLATMVEFTASEACRTSLCSADLYGWTGLEPADDAAYDPIRHIQDTL
                     ELEAADLWAELD"
     gene            complement(8928..10280)
                     /locus_tag="PK829_01095"
     CDS             complement(8928..10280)
                     /locus_tag="PK829_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39838.1"
                     /translation="MFSKRTAPILVILVAAALVLLAVWLAGRVLNGDDSLLRNASVRD
                     AAITPNADGDTDATLIRYELSRNASVSIYLENAAGQRFYFRQDKPRGAGEYEVLFSGV
                     VDGYQLAGETIDGDILARLLRDGDYTWTIVATDLAGVTETQSGALTISAADPQLPEIR
                     NFSLDKDTFTPNRDGIADRILAQLYLPKEVASLRVYLQMADGSEAPIAEQERDIPLNM
                     PGRHYFDYEGGVDNGETPPPDGTYPLIAVAEDLEGQRVRVESELTIQFGGVPRGYIIS
                     PPTGNTVQWSATAVSLCDTITFTLTVENDGNTPIRTTGPAPGTVYDSDWNYNTVGWPT
                     ESGAFRVAIGFENELTNYPYRWAVGNVEDLQRIGDYYYLMPKQRAVITGSIRVVDVFG
                     VRNPQPIWAGLIHEDVEITAFNDRVDTQAILVDVPDANNMPTCAPRAIPVRPLTGAKQ
                     "
     gene            complement(10368..11555)
                     /gene="lgt"
                     /locus_tag="PK829_01100"
     CDS             complement(10368..11555)
                     /gene="lgt"
                     /locus_tag="PK829_01100"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU39839.1"
                     /translation="MAARRRIEPYWYAIGLVWLALAALFVTHLLTGFTPARAAVSLFG
                     FAIYWYAICILVGVGLGTWVVARLADRRARAIFETTVPAEVRARPLAEVSLPEGAAAL
                     LARRKLATLGDLLYEVGLDPRRLGLKLPDWAAVDAALRATPDVDPAWLSNTPWRVWNP
                     DHAWGGLLWMLVLGLLGARLYHVLTPSPSMAAVGIYSWRDYFRYPLQLLNFRSGGLGI
                     YGAMVGGLLAVLLYTRRHRLPALAWADLAVIGAALGQAVGRWGNFFNQELYGRPTSLP
                     WAITIDPLHRLPDYADFSTFHPAFLYESLWSLLTFGVLLALHHRRGQRLLPGELLALY
                     LVAYAIGRSLLELVRLDSRAVPFLGLETGLAVATVVSLLVALAAVVAVVVRRARRRRL
                     SGG"
     gene            complement(11567..12178)
                     /locus_tag="PK829_01105"
     CDS             complement(11567..12178)
                     /locus_tag="PK829_01105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU39840.1"
                     /translation="MNQDSSLPPRPTLTDQLRARTHFIIDPIVTTMARFRLSPDLLTV
                     VGALAHVLFAYLIGIGAFRWAALAIVLVSPLDALDGALARKVGRKQGGFGAFLDSSLD
                     RLAEIVLFGGFIYYYYTHNDPRMMAIAYLAVTGSLMVSYARSRAEALGYSAKMGVLSR
                     VERYIVLVVFLALGMPQIALPILALFTYFTFFQRMFAVWKQSR"
     gene            complement(12405..13112)
                     /gene="atpF"
                     /locus_tag="PK829_01110"
     CDS             complement(12405..13112)
                     /gene="atpF"
                     /locus_tag="PK829_01110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOU39841.1"
                     /translation="MEALGINLGYLIVQILGIIFLLLILKGFVYQPMLRVLDERKARI
                     AKGLEDALQADIARQNADADAKRILDEARSEAANIRREAVVAAEQAAKDVEMRAREDA
                     KGIMANAQTEATEERNRILADLRGQVAAISIAAANKLVGESLDDRRQRQLINEFFSRV
                     PADVASLKGDAAEVTSALPLTAAEQESVRRTVGAQTVQFKVDPSILGGLIVRVGDRVV
                     DDSVANRMSALQESLRR"
     gene            complement(13140..13367)
                     /gene="atpE"
                     /locus_tag="PK829_01115"
     CDS             complement(13140..13367)
                     /gene="atpE"
                     /locus_tag="PK829_01115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOU39842.1"
                     /translation="MLAQGLKAMGAGLAMTGAIGAGAGIGILVGGAVQGMARNPDAGG
                     TILTNMILGIAFAEAVAIYALVVALIILFVF"
BASE COUNT         2311 a         4412 c         4504 g         2160 t
ORIGIN      
        1 aaatcatggg cattagcagc cctggccgcg tctacgacaa catccgctac ggccgcctgg
       61 aagcgtcgcg cgaagagatc gaggccgcgg ccagggctgc taatgcccat gatttcatca
      121 tcagcgactt gagcgagggc tatcacacgg ccgtcggcga gcacggcgtc aagctcagtg
      181 gcggccagcg ccagcgcatc gctattgccc gcgccattct gaaagacccg cgtatcctca
      241 tcctcgacga ggcgacttcc tcgctcgaca gcgccagcga gagcctggtg caggaggcgc
      301 tagagcggct gatggtcggc cggacgtcgt ttgtcattgc ccaccggctg agcaccgtga
      361 tgaacgccga ctggattctg gtgatgaacc ggggtgagct tgtggagcag ggcacccatg
      421 cctcgctatt gctgaacccc gacggcctat atgcacaact gcaccggatg cagttcgccg
      481 agaacagacc ggcggcggtc taactggcgg tggcctgtcg ggcggtggcc tatcggtagc
      541 tgcctgggac tggaaataaa aacagccgtc cagaattgga cggctgttat catccgggtc
      601 agccgtgctg ccaacagtgg gcttgccagg cagcgagcga gcgattagcc gcgcgtgcgc
      661 gccgtgttgt cggagggagc ttcccgtgtg tcggtgatga aggtctcgac gtagttgtcg
      721 tgccggttca tgcgcgagga catgatgccc gcagccagaa cgaagatcga gctaaataca
      781 gacgcaccga ggaagaaaag cagccacatc atctctcatt acctctacaa ccggttcttt
      841 tccggaatct actttatcgt aagaaaagtc gtaaggttca tcgactagac tttgctacct
      901 acgatgcccg cattatagcg gttaataggt accttgtcta cccctccagg ccccaaacgc
      961 ggtaacaaag cgataacaat caaccggcgc tcatcttcgg cctagcggcc gtgccattcc
     1021 gcccggcgct tgttgatgaa ggcatccatg ccctctttct ggtcctcggt gctgaagagc
     1081 aagtagaaga gacggcgctc gtggtctaac ccagcgcgca gcgacatctc gaaagtggca
     1141 ttgatagcct ccttggccag ccgcacggcc acgggcgcac gggcggcgat ttcgttggcc
     1201 agccgcacgg cttcctccag gtagacctca accgggtaga catggttgat aaggccggcg
     1261 cgccgcgctt cctcggccga gagatagcgc ccattgagca ccatctccat ggccacagcc
     1321 ttgcccactg ccagtgtcaa ccgctgcgtg ccgcccgcgc cggggatgac gcccaggtta
     1381 atctccggct ggccaaactg ggccgtctcg ctggcaatga tcaggtcaca ggccatggcc
     1441 agctcgcagc cgccgcccag ggcaaagccc gacacggcgg cgatgaccgg cttgccgatg
     1501 tggtgcagcc gatcccacag atcgatgaac gagccggcca tcatctgcgc cggggtggcc
     1561 gcagccatgg ccttgatgtc ggccccggcg gcgaaggcgc ggtcactgcc agtcacgacc
     1621 atgcagccaa cggcggggtc ggcgtcgaac gcttccagcg ccgtcatcag ctcagtcatc
     1681 agctcgcggt tcagcgcatt gagcgctttg ggccggtcga gttgggccac gccggcgcgg
     1741 ccctccacgc gcgtaaggat gttttcgtag gtcgtcatgc caatctcctg tagctttctg
     1801 gcgcgcttgc gccctgttgg ccccgctcca tgcgcctgcc ggctgtcgct ctcagatgcg
     1861 gtaatccgtt tcctgccgcg gccacagccg ccagatgatg atcaggaata gcagaccggt
     1921 agccaggaag caggtctgaa ggcccgctat cttcaagtcg tcgggcggtt gaaagacgaa
     1981 ccagacagtg aaggtttgta gggcctgcac caggatggtt cccaggatgc tgcctgtgcg
     2041 caggcggatg atgccataga gcacgcccca cagcaggaag tagagcaggc tggtcagtcc
     2101 ggccagcaca gacacgtcca taccggctac aaacgactcg ccaaagaggg tgaagccgac
     2161 caggccgaag agcaggccgc tgacgatggc cgctgtcagg ccgccgcgat agtcggccaa
     2221 ggctcgaaag accagaccaa aggcccacaa ttgcacggcg aaggcctcga agagcagcag
     2281 ataggcaaag gcgcggccgg agccggccga gcgaatggcg acgatctcaa ccaacatgta
     2341 gcggccaata agcgccacca cccaggccaa gaccgcgaag ccgatgccgg cgaagagcgg
     2401 ccgcccgccg cgcagggcca tccctcgcag gccatagagc ttcaggccca ggaaccaact
     2461 ggccagaccc agcgcggcga agagtggcgc ggcgctggcg ctggcgactt cgccgcgctg
     2521 gaagagcgcg ccggtgatga ttcccagcag cagagccgcg gccaacggca acaagatggc
     2581 caggagaacg cgattgcggc cgacgatggg cgcgaccggt tcgcgggccg gcaggggggg
     2641 cgaggttgcg ccaagagttt tgcgaggcat gctcggattg tagcaccatc cccggcttgg
     2701 gctaaactaa caggctgatg aaccactcgc ccgatagctc gcccgccgcg ccgccggccg
     2761 acctttccgc gctgccggcg gccgactggg gctgggacgc gcgccgcgcc atccaactgg
     2821 gcattctcac cttcgcccgc ttctgcctga acacgatgct gcgcgtcgtc tatccctttg
     2881 cgcccgcctt cgcccgcggc ctgggcgtgc cggtgacgac catctatctg ctcatctcgt
     2941 tgcgcaactt caccggcctg ctcagccccg tctttgcgcc gctggctgag cgctacggcc
     3001 gccgcaatgt catgtccctc agcatggccg tcttcagcgc cgcggccctg ctggtggccg
     3061 gcgtgcccgc cgtgtggacg ctgggcgcgg ccctggtggt tgtcggcgcg gtcaaggtca
     3121 tctacgaccc ggccatgcag tcctacctgg gcgatgccgt gccctaccaa cagcgcggcc
     3181 gggccttagc catcaccgag ttcggctgga gcggcgcgtt tctgatcggc gcgccgctga
     3241 cgggctggct cattggccgg cagggctggc cggccccgtt cctgtggctg ggcattggcg
     3301 gcgcagtcgg gacggtgctg ctctggcgca ccttgccggc cgcccgccgc cacgcccacg
     3361 gcacgatcac catcggctac acctggcaga tgttgcgccg ccacccgatt atctgggccg
     3421 cggcgctgta catgctcctg ccgctcattg cccaggaggc gctgttcatc gtctacggtg
     3481 actggatgga gagcactttc aacctgagtc tgacggggct gggcctggcg acgacgctca
     3541 tcggcctggc cgagatgagc ggcgaggcca cagccggctg gtcggtcgat cgctttggca
     3601 agcggccggt cgtcgccctc agcggcgtgc tgaccgcggg cgtcttcctg ctcattcccc
     3661 tcagcgccgg cagcctgacg accgccctgg cggcgctggt ggctctcttc ttcctgttcg
     3721 agatcgctgt cgtcggggcc atccccctgc tgaccgaact ggtgccggag gcgcgcggcg
     3781 tggtcatgtc gatgagtttc ggcgcgatgg ccgccgggcg caccatcggg tcgatcatcg
     3841 gcccggccgt ctggctgcgc gtgggcatgt ggggcaatag cctcatcgcc gccgtggtga
     3901 tgttgctggc cgtcgtcgtc ctcgcgcgct ggctgcgcga ggccaaccca ggaggcgcgt
     3961 gatggagatc gtcgatctgc tcgtttactc cgccggacaa gtctgcgtgg tgccgcccca
     4021 cgatggcccc cagcgcggcg cggccctggg cgatctgggc ctgctggccg gcggcgcggt
     4081 ggccgtggac ggcggacgga tcgtcgccgt ggggccgacg gccgagctgc aagcgcgatt
     4141 tcgggccgag gcgatgatcg acgccggcgg gcgttgcgtc gtgcccggct tcgtcgatcc
     4201 ccacacccac ctgccctggc agggcgaccg cgccactgag tttgagcagc gcctggccgg
     4261 agccagctac atggagatca tggccgccgg cggcggcatc atgtccaccg tccggcgcac
     4321 ccgcgccgcc agcgtggccg acctggtggc caacaaccag ccgcgcctgg cgcggatgct
     4381 gcgccacggc acaaccagcg ccgagaccaa gaccggctat ggtctggaga cggcggccga
     4441 gttgcgccaa ctcgacgcta tcatggccct gagcgcggcc cagccagtgg aactgacgcc
     4501 gaccttcctg cccgcccacg ccgtgccggc cgagttcgcc ggcgacaccg acggctacgt
     4561 ggactacctg gtggacgaca cgctgccggc cggcgcagca tggatgcgcg cgcggggcgt
     4621 cgtcctcttt tgcgacgtgt tttgcgaggc gggcgtcttc gacctggcgc agacgcggcg
     4681 cattctggag gccgccgcgg ccctgggcta ccggctcaag gttcacgccg atgagttcgt
     4741 cggcctgggc ggcacacggc tggcggtgga attgggcgcg gtctcggccg atcatctggt
     4801 cagcacgccg gccgaggaca tcgccgcgct cggtcggggc gacaccatcg ccgttggtct
     4861 gccgggcacg ccctttggcc tggcccaccg cgactacacc ccggccaagg ccatcctggc
     4921 ggcgggcggc gcgctggccc tggccaccga ctgcaacccc ggcacgtgtt ggtgcgagtc
     4981 gatgcagatg gtcatcgccc tggcctgccg ctacatgggc ctgacgcagg cgcaggcgct
     5041 ggccgcggcg acgctcaacg ccgcctatgc catcggccgc ggccgcgagg ttggcagcct
     5101 gacgccgggt tacgccgccg acctgctcat cctcgacgtg gccgattatc gccagttggg
     5161 ctatcgcttc ggcacgaacc tggtgcagac ggtcatcaag cgcggccgcg tcgtggcggt
     5221 caatggcctg gagggggaat ggacggagta aacctgacaa cagccggctg gatcatgctg
     5281 gcggtgatcg tgctggcgct gctggccgtg gccgtgcgct acttcatcag cgtggcgatg
     5341 gagttcacgc gggtgctgct ggtggccggt ggcgtgctgc tggtggccat cctcttttgg
     5401 attctattcg ttcactgagg caatgaaccc gccggagtct tcccggtgtc cattggcgtc
     5461 atcggcgtgc tcagggcttt accaaagtca cccaggcgga ccgacctgac aggtaggcgg
     5521 ccacctgtca ggtcaatggt cgctaggcaa ctttggaaaa gccctgtcgg cgtattattc
     5581 ttccccgccc gcgtccgcgc tataatcgcg gctatgaact atcgtatcgt gacgctcctg
     5641 gcgctgctgt tattattggt ggctggctgc cgccaaaacc ccgcggcgac cggcgaaacc
     5701 gcgcctgcga ccccggccat cacggccacg cggcccccgc ccgtggcgtc cacgtccacg
     5761 cccgtcccgc cgacgccaac gcccactgag ccgctggccg ccctggtcaa tggcgaagac
     5821 attactctgg ccgcctttga gcgggagctg gcccgcttca cggcggcgca gccagacgcg
     5881 cccgccgccg acgccgccca acaggtgctc gacgcgctta ttgagcggca actcatcgtt
     5941 caggcggccg cggccgaggg cgtcgctgtc gccccagaag ccgtcgaggc caagctggcc
     6001 gagttgcgcg ccacctacgc caccccggcc gagttcgaca cctggctaca gaccaacggc
     6061 tacaccgaag acgaattccg cgcggcgctg gcggcggaaa tgaccgtcgg ggagatgacc
     6121 gcgcgggtga cggccggcgt gccgacgacg gccgagcagg tgcgcgctcg ctacatccag
     6181 atggacgacg ccacgctggc ccagtccgtg ctcgaccgcg cccgcgccgg cgacgacttc
     6241 gccttcctgg ccgagcagaa ctcggtcgat cgcgttacgg ccgagatcgg cggcgatctg
     6301 ggcttcttcg cgcgggggtc cttgctggtg ccagaggtgg aggcagccgc ctttgccttg
     6361 caaccggaag agatcagcga cgtgatcgcc gtcactggcg cggacggcaa gacgatctac
     6421 tacatcattc aggtgacaga gcgcgacccg gagcgggaac tgacggccga cgccaccgcc
     6481 gccctcttgc aggcggcatt cagcgattgg ctcgacggcc tgcgggcagc ggcgacgatt
     6541 gagcgcttcg tgccttaggc caggcccttg cgaccggccg cgtcggccga ccaggccagc
     6601 acaatgcgct cgtaggcgtt cttgagttgc gggtcctggg ccagacgcgc ccggcgggcc
     6661 atcttgcggc gcaccagcca gccagcgacc cgctgcttca gcggcccatg gtgcttgcga
     6721 tagagctgcg cccggctctc ccagagattc agcatcgact gcgccggaat ctgccgcgaa
     6781 ctctcgccgc catagtggac gatttccgcc tccggcacga cgtagatctc ccagcctgac
     6841 tcgcgcacgc gccaactcca atcgatctcc tcgcagtaca tgtggaagga ttcgtcgaag
     6901 ccctgggtcg attcagcgac gtcggcgcga acgagcatcg ttgcccccag ggggtgatcg
     6961 acggcaaagg gctgccggtt aggctggaag aggcgacgcg gatagcgacc attgagccgg
     7021 ctgtcgtaga ggcgtggcgg catggcgtag aggtcgaaaa ccaactgccc cagcccgggg
     7081 aaggaaaagg cgctgtgctg gaaacggccg tcgccaaaga tgagccgcgc gctggccata
     7141 ccccccttgg gccggtcgtc caggcacttg gccatccggc caatcgctcc cgggcgcacc
     7201 agcgtgtcgg ggtttagaaa gaggtagtag cgcggccggc tggcagcggc ggcgcgcatg
     7261 ccctgattat tggccgcgcc aaaaccgacg ttagtctggt tggcgatgac gtgcacctgc
     7321 gggaacagat cttccaacaa cgccaccgtg ccatcggtgg aggcgttgtc gatcacccaa
     7381 atctcccccc gcagccgcga ggcgcgcaga tcggcggcaa ccgagcgcag gcagtccgac
     7441 agatagttgc gaacgttcca actgacgatg atgatggcga cgtcgatcat ctcagtccag
     7501 ttctgcccat aggtcggcag cttccagttc cagcgtgtct tggatatggc gaattgggtc
     7561 ataggcggca tcatcggctg gttccagtcc ggtccagccg tacaggtcgg ccgagcacag
     7621 cgatgtccgg caggcttcgg acgcggtgaa ctcgaccatc gtcgccagcg tctgccgggc
     7681 cagactgagg ggaaagtccg cgccaaagac gaccgtctca ttggggatcg gctcgctcag
     7741 cgccaggata cgggtcacgt caaagatatc gggcgtcgtg tcgaacagcc gggcgcgcgc
     7801 gtcgcggagc cggtagccgc caagctcagg ctccccggcg acgagcacat agccgatggg
     7861 gctacgcgtc ggcgcaatgc ccaacacgcg ccactcctcc gggtccgtct ccccgtagat
     7921 ccattgccgc tccagcggca tgatgggcgg gacgaagtcg gcggtggtga aatcgacctc
     7981 ctgatcgcgc agggccagca gggcgctggt gtcctccggc gtggtcacga cctcacccgg
     8041 ctcgatgccc gcttcggcca tctgggcgcg gaagtagagg tagttgggca ggctgcgcgt
     8101 gtcggccaca gcccagcgcc ggccgtccag gtcatccagc gcgccggccg cgcccgggcg
     8161 ggtcacgagc atgcccatct gccaggtcag gccatcgtcg tggcgggcca ccagcccggc
     8221 caccgcgccg cactcgtcat gggccacggt gtaggccgcg gcggagacga aggcgatgac
     8281 gtcggccggg gccgcgcaca gcagcgccgc cacagcggcc tcgttgtcgg ggataccgac
     8341 ggcgaactcc gcgcccgtgg ccgcctccag cgccgccacc agtggctccg tgcgctgggc
     8401 aatcacagcg gcggtcgttg taggcagaaa gagcagttgc accggccgct cggccgtgcc
     8461 caggggcggg cgggtgacct cgacggtgac ctcgacaggg acgggcggcg cggcgacggt
     8521 gggcgcgctg gtggccgcgc cagccagcgc cgtcgtgggg gaaggtggcg cagccgtctc
     8581 gctcggcgcg gccaccacca ggcgcgtcac ctcgaccgtg acggcttccg gtcggcagga
     8641 cggcaagaag gcgatcgcgg caagcaatga cgcgctgaga gtgatggtga tgtgatgtcg
     8701 tttcatggat aaaaaacgac tgacgtgggc cgtcagtcgc ttgcctgtta tcaaatcctg
     8761 tacggggtac gggatcacgc ccactctgcg cgaatagcgc cagccaacca caatgacctt
     8821 agtgtatcca cttgtaatat gccctccaat gacattcagg ggaggctaag gaagtagagc
     8881 ggcgcggcag gtgttcactg cccccaggag cgtagtcacc aggcggatta ctgcttggcc
     8941 cccgtcagcg gtcgcacggg aatggcccgc ggcgcgcagg tgggcatgtt gttcgcgtcg
     9001 ggcacatcga cgaggatcgc ctgcgtatcg acgcggtcgt tgaaggcggt gatctctacg
     9061 tcctcgtgga tgaggccggc ccagatgggc tgcgggttgc gcacgccgaa gacatcgacc
     9121 acacggatgc tgccggtgat gacggcgcgc tgcttgggca tcaggtagta gtagtcgccg
     9181 atgcgctgca agtcttcgac gttgcccacg gcccagcgat aggggtagtt ggtcagctcg
     9241 ttctcgaagc cgatggccac gcggaacgcg ccggactcgg tgggccaacc aacggtattg
     9301 tagttccagt cggagtcgta gaccgtgccc ggcgcggggc cggtggtgcg gatgggcgtg
     9361 ttgccgtcgt tctccacggt cagggtgaac gtgatggtgt cgcacaggga cacggccgtg
     9421 gcgctccact gcaccgtgtt gcccgtcggc ggcgagatga tgtagccgcg cggcacgccg
     9481 ccgaactgga tggtcaactc gctctcgacg cgcacgcgct gcccctccag gtcttcggcc
     9541 acggcgatga gcgggtacgt gccgtcgggc gggggcgtct cgccattgtc aacgccaccc
     9601 tcgtagtcga agtaatggcg gccgggcatg ttcagcggga tgtcgcgctc ctgttcggcg
     9661 atgggcgctt cgctgccgtc ggccatctgc aggtagacgc gcagcgaggc gacctccttg
     9721 ggcagataga gctgcgccag gatgcggtcg gcgatgccgt cgcggttggg cgtgaaggtg
     9781 tccttgtcga ggctgaagtt gcgaatctcc ggcagctgtg ggtcggccgc gctgatggtc
     9841 aacgcccctg attgcgtctc ggtgacgccg gcgaggtcgg tggcgacaat agtccaggta
     9901 tagtcgccat cgcgcagcag gcgagccagg atgtcgccgt cgatggtctc gcccgccagc
     9961 tggtagccat cgacgacgcc gctgaagagc acctcatact cgcccgcgcc ccgcggcttg
    10021 tcctggcgaa agtagaagcg ctggccggca gcgttctcca gatagatgga cacgctggcg
    10081 ttgcgcgaca gctcatagcg gatcagggtg gcgtcggtgt cgccgtcggc gttgggggtg
    10141 atagcggcgt cgcggacgct ggcgttgcgc aacaggctgt catcaccgtt gaggacgcgg
    10201 ccggccagcc acacggccag cagcaccagc gcggcggcga cgaggatgac gaggatggga
    10261 gcggtgcgtt tcgagaacaa cggttcaacc tttcgttcgc cgccggagat ggccaggcga
    10321 gcgacagccc taagtgtagc agacgcacta cccccggcca gcgacgatta gccgccgctt
    10381 agccgacgcc ggcgcgcccg ccggacgacg acagccacca cagccgccaa tgccaccagc
    10441 aacgagacga cggtggcgac ggccagcccc gtctccagcc ccaggaaggg cacggcacga
    10501 ctgtccaggc gcaccaactc cagcaggctg cggccgatag cataggccac gaggtacagc
    10561 gccagcagtt cgcccggcag cagccgctgg ccgcgccggt gatggagcgc cagcagcacg
    10621 ccgaaggtta gcaggctcca tagcgattcg tacaggaacg cggggtggaa agtgctgaag
    10681 tcggcgtagt cgggcagccg gtggagcggg tcgatggtga tggcccacgg cagactggtc
    10741 ggccggccat agagttcctg gttgaagaag ttgccccagc ggccgacggc ctgccccagt
    10801 gccgcgccga tgacggccag atcggcccag gccagtgccg gcagccggtg gcggcgcgtg
    10861 tagagcagca ccgccagcag cccgccaacc atggccccgt agatgcccag accgccgcta
    10921 cggaagttga gcaactgcaa cgggtagcgg aagtagtcgc gccacgaata gatgcccacc
    10981 gcggccatgc tgggcgaggg cgtcagcaca tggtaaaggc gcgcgccgag gaggcccagc
    11041 accagcatcc acagcagccc gccccaggca tggtcggggt tccagacgcg ccagggggtg
    11101 ttgctgagcc aggcggggtc aacgtcgggc gtggcgcgca gcgcggcatc gacggccgcc
    11161 cagtcgggca acttcagtcc cagccggcgc gggtcgagac cgacttcgta gagcaaatcg
    11221 cccagcgtcg ccaacttgcg gcgggccaat agcgccgccg cgccctccgg caggctgact
    11281 tcggccagcg gccgggcacg cacctcggcc ggcacggtcg tctcgaagat cgctcgcgcc
    11341 cgccggtcgg ccagccgcgc cacgacccac gtgcccaggc cgacgccgac gagaatgcag
    11401 atggcatacc agtagatggc gaagccgaag agcgagacag ccgcgcgcgc cggcgtgaac
    11461 ccggtcagca ggtgggtgac gaagagcgcg gccagggcca gccagaccag gccaatggcg
    11521 taccaatatg gttcaatacg acgtcgcgca gccacgaccg tccctcctac cgcgactgct
    11581 tccagacggc gaacatgcgc tggaagaagg tgaaataggt gaagagggcc aggataggta
    11641 gggcgatttg gggcatgccc agggccagaa agaccaccag aacaatgtag cgttccacgc
    11701 ggctaaggac gcccattttg gctgagtagc ccagcgcctc ggcgcgcgac cgggcatagc
    11761 tgaccatcag cgagccagtg acggccagat aggcgatggc catcatgcgc gggtcgttgt
    11821 gggtgtagta gtaatagatg aagccgccga agaggacgat ctcggccagc cggtcgagcg
    11881 acgagtcgag gaacgcgccg aagccgccct gcttgcgccc caccttgcgc gccagcgcgc
    11941 catcgagcgc gtccagcggc gacaccagga cgatggccag cgctgcccac cggaacgcgc
    12001 caatgccgat caggtaggcg aagagcacgt gcgccagcgc gccgacgacg gtcaacaggt
    12061 cgggcgagag gcgaaagcgc gccatcgtcg tgacgatagg atcgatgata aagtgcgtgc
    12121 gggcgcgcaa ctggtcggtc agggtcgggc ggggcggcaa tgaactatct tgattcatat
    12181 cagcgatctc tacgattccg ggtggatgct aacgccgggc ggcaggcctg tcaaggagcg
    12241 ctcataacaa aacaggccag gcgactgccg tcgcccggcc tgttgcttgg gccaacctgt
    12301 caaccagcaa aagacgttgg cggccgtttc ggcgcggccg ccgcttctcc cctctcttaa
    12361 cgagagcgca ttcgatagcc aatgggcacg ccggcaggcg cgctttaacg acgcagcgac
    12421 tcctgcaggg cgctcatgcg gttggcgacg ctatcatcga cgacgcgatc acccacgcgc
    12481 acaatcagcc cacccaggat cgacgggtct accttgaact gaaccgtctg cgcgccgacc
    12541 gtccggcgca ctgactcttg ttcagcagcc gtcagcggca gggcgctggt cacttcggcg
    12601 gcatcgccct tcagcgaggc gacatcggcc ggcacacggc tgaagaactc gttgatgagc
    12661 tggcgttgac gccgatcgtc cagcgattcg ccaaccagct tgttggccgc ggcgatggag
    12721 atggccgcca cctggccgcg cagatcggcc agaatgcggt tgcgctcctc agtggcttcg
    12781 gtctgagcgt tggccatgat acctttcgca tcttcgcgcg cgcgcatctc gacgtccttg
    12841 gccgcctgct cggcggcgac gaccgcctcg cggcggatgt tggccgcctc agagcgggcc
    12901 tcgtccagaa tacgcttggc gtcggcgtcg gcgttctggc gggcaatgtc cgcctggagc
    12961 gcatcttcca accccttggc aatgcgcgcc ttccgttcat ccagaacgcg cagcatcggc
    13021 tgatagacga agcccttcaa gatgagcagc aagaagatga tgcccagtat ctgtacaata
    13081 aggtagccta gattgattcc cagagcttcc attcgtaacc tctttgtgcg gttcgtcgtc
    13141 tagaagacga acaggataat cagggctacc acaagggcat aaatggcgac cgcttcggca
    13201 aaggcgatgc ccaggatcat gttcgtcagg atcgtgccgc cggcgtcggg gttgcgggcc
    13261 atgccctgca ccgcaccgcc caccagaata ccgatgcctg cgccagcgcc gatagcgccg
    13321 gtcatcgcca ggcccgcgcc cattgccttc aggccttgcg ctagaatagt tgcagattcc
    13381 acatcca
//
LOCUS       DAOSMQ010000016        12851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_4864_length_12851_cov_5.090731, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000016 DAOSMQ010000000
VERSION     DAOSMQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12851
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4864_length_12851_cov_5.090731"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..292
                     /gene="rpsO"
                     /locus_tag="PK829_01120"
     CDS             23..292
                     /gene="rpsO"
                     /locus_tag="PK829_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU39843.1"
                     /translation="MPLTVAEKAELIDNFATHDGDTGSPEVQIALMDKRIRQLQEHLN
                     THKHDESSRRGLLKLVGKRRRMLAYLRKEHPDRYRTILGRLGLRR"
     gene            619..2847
                     /locus_tag="PK829_01125"
     CDS             619..2847
                     /locus_tag="PK829_01125"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU39844.1"
                     /translation="MNSESVFSTTVGRHKLTIATGKLAEQAGGAVTLQVGESVLLATA
                     TMSKNIREGLDFFPLSVDFEEKMYAAGRIPGGFFRREGKPTTEAVLTSRLTDRPLRPL
                     FPDGMRNEVQVIVTTLSSDSIHLLDIMSVNAASAALTISDVPWGGPIGAVRVGYIDGQ
                     FVVDPTVEEMAESQLDLRMAGSRDAIIMVEAAANEVPEELMVKALAFGHEALQPIITL
                     LEEMRAAVGKEKREPTIVVSDAALAEMTKGRIGGRVAEVIASTTEREARNEALDALRD
                     EVVDSFLADDETTDPKAVREVFADEIKKVVRDRILYDGVRPDGRDYTTIRPLSSEVGL
                     SPRAHGSGLFQRGQTQVLSIVALGTPREAQKLDGLSPEDSRRYMHHYNFPPFSTGETW
                     PMRGPRRREIGHGALAENALLPMIPSEDEFPYTIRVVSEVLSSNGSTSQASVCASSLA
                     LMDCGVPIKRPVAGVAMGLVTDGHKYAVLTDIQGMEDHLGDMDFKVAGTSEGITALQM
                     DIKITGLDMDIMTRALEQARVGRLQILDSMLATIAAPREQLSPYAPRMLTIKVDPEKI
                     GAIIGKGGATIRGLEEEFEVSVDIQDDGTVFVAGVDGLKAEAALERIHALSHDPEPGE
                     VFTGKIVRITDFGVFVELTPGQDGMVHISQLASEHLQRVEDAVRMGDEVMVMVTDVSP
                     EGKIRLSRRAVLEGWSLDEARAQDAARKSSGGPRSNGGGGRDRGGDRRGGGDRGGRPP
                     RR"
     gene            3020..3292
                     /locus_tag="PK829_01130"
     CDS             3020..3292
                     /locus_tag="PK829_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39845.1"
                     /translation="MRRFSWLLIPLLWLLAACGSGADQFSNPTAVAGPGEDSPPTATV
                     RATTAADSTAVPAANADDPTQARERDWKQGDLEDPAVTIIEYGDFQ"
     gene            3311..3724
                     /locus_tag="PK829_01135"
                     /pseudo
     CDS             3311..3724
                     /locus_tag="PK829_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            3833..4285
                     /locus_tag="PK829_01140"
     CDS             3833..4285
                     /locus_tag="PK829_01140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU39846.1"
                     /translation="MANGGVFTIELLPKSAPQTVNSFVFLAREGWFDGVTFHRVLPGF
                     VAQTGDPSGTGRGGPGYMIPNEIDSSLSHNEAGVVAMANAGPNTNGSQWYITYGDVSQ
                     LDGSYTIFGRVIEGMDVVEAITPRDPSRNPTAPAGDAIAHVTIEEIQP"
     gene            4398..5900
                     /locus_tag="PK829_01145"
     CDS             4398..5900
                     /locus_tag="PK829_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39847.1"
                     /translation="MTYRFQLPAEPLVERNPFRQGERRSSLTNFADVRSCLPVPVFPQ
                     QPGWERLYWTAWEHLWSCLRAPATNSRLIAPYLQPSGDQPVEMAPAAFVSQLAGYIPG
                     STALIDPLDNFYARQHDTGFICRAFNPDDGRDFHQPYEPNSTGPALLAWGEWRRFRLT
                     GDASRIADAFWPLLAYHRWCRANRTWPDGLYWATAYSSGLTNQPRVPGGRTHHAHWTW
                     VDASAQATLDAALLERMAVLLGEQALADEVSAERVMLIQRFNATLWNENHSFYQDAGP
                     DGRFSPVRSIAAYWALLDGHLVPKERLTPFIQHLRDTWSFRTPMVLPSMAADSEAYNA
                     RTGNGWRGGVWPTLTYMVLRGLGLNEQHVLAHKLALTHLDVVGQLFETTGRLWSHYAP
                     EDLSPGDPSEEDAAGQTAAAIIAMLLENIVGVWVDWPLRQVTWRRHLQGAEEYGVRQL
                     PLGNEGTLDLMSAGNLVRVRTDAPLTLVLHNGSEVVRTAVPAGAFEIALD"
     gene            5919..6701
                     /locus_tag="PK829_01150"
     CDS             5919..6701
                     /locus_tag="PK829_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU39848.1"
                     /translation="MFAFTVATINLHNRADRWLARRHLMVGQLMDVQPDIVALQEISL
                     TIGQATWLARQINTRLTGKSNQPYRVIQAPRASARHRFEGVGVLTRLPVIYHDVLPLG
                     FDGRIALRVNVELPAGAAGSRRQSLDFVSAHLHHVATDRDARLEQAMILVGWLNDKRR
                     VPLQVIAGDFNEAPDGPAIAFMRQSYRSVYAEVKGRDPLATFPTNLVRPPTDWAACLD
                     YVFVSPAVYKVTRAAIFLDKPASDDDTLYPSDHVGLLVGLEV"
     gene            6703..7722
                     /locus_tag="PK829_01155"
     CDS             6703..7722
                     /locus_tag="PK829_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU39849.1"
                     /translation="MSAEERHDLISIVAPVYNEEGVLDALYERVRAVLDGAGEVWELV
                     LVNDGSRDRSADVIARLHAQDPRVKGLSFSRNFGFQIAATAGLDYARGDAVILTDADL
                     QDPPEVYPALLAQWRAGYDVVYGIRASREGETWFKLATAKLFYRLIHRITSINIPLDT
                     GDFRLLDRRVINALRGMNERNRFLRGMVPWVGFRQIGVEYHREARYAGEAKFTSVKKM
                     LPFALDAITSFSYFPLQLATILGFVMATISAVAILAVVLVRLLGPHEPLLGQATTLVA
                     VLFLGSVQLICLGIIGEYLGRIYDEVKGRPLYLIQQSWGIDNAPLGRFAPPTGPSSGA
                     EQKRA"
     gene            complement(7719..8699)
                     /gene="moaA"
                     /locus_tag="PK829_01160"
     CDS             complement(7719..8699)
                     /gene="moaA"
                     /locus_tag="PK829_01160"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOU39850.1"
                     /translation="MSIDRFGRNINYLRISLTDRCNLRCVYCMPEDMTFRPRAELLQD
                     EEMLRLVRLFAELGFHKFRLTGGEPTVRANVVDLVRQMAATPGVDKIAMTTNGLLLDR
                     LARPLAEAGLQRVNISIDTLDPDRFKKLTRWGKVEEVWAGIRAAEAAGLEIKLNAVIV
                     RNYNDREDLVDLARLTLHQPWQVRFIEMMPFGDVADFQLAGVVSEDELRATIAAHLGP
                     LRPVNDGHLDGEASLYQLDGAPGTIGFISSVTHPFCASCNRARLTADGRLRLCLLREK
                     EVDLLTPMRAGADDEEMRATITAGIWWKPWGHGLADDVIPLNRVMSEIGG"
     gene            9008..10066
                     /locus_tag="PK829_01165"
     CDS             9008..10066
                     /locus_tag="PK829_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOU39851.1"
                     /translation="MYKQPLLPGWLSALLSAAFVFSAVAVLFFAYLTVQHLMSRPATM
                     VDAAGAEGGLSPVGGDLPPAQVIDGGTAPTPTPAVPTVAAWDGDSRVNILLMGIDRRP
                     GESFVSRTDTMMILSVDPETDSAVILSVPRDLYVQIPGFGEDRINTALVLGARNGDYL
                     DGAALAMQTVSYNLNIPIHHFVLVDFNAFVRSIDLLGGVDVDVPYEINDPEYPDMDYG
                     YDPLYIPAGLQHFDGAMALKYARTRHGDTDFNRAFRQQQVLFAARQQVVKLGIGEMLL
                     RAPSLYREVEEGIRTDLSLEQMLRLAKTISDIPSGNIRNDVLDYDYVSSYRTPGGASV
                     LLLRPETAVPLIESLFGQ"
     gene            10087..11955
                     /locus_tag="PK829_01170"
     CDS             10087..11955
                     /locus_tag="PK829_01170"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOU39852.1"
                     /translation="MNRQEFKQRLEQGPVLMDGAFGTVLHGRGIPIDQSFDAVNLTNP
                     ALVAEIHRGYIDAGSDLIETNTFGANHYKLAEHGLQDQVAAINQAAVAIARRVIAGSF
                     REVLLAGSIGPLGVRLAPLGRVPMTEAEAAFREQIEALAAGPAGVDLLIIETMSDIKE
                     IEAAVNAARAVAPELPVIAQMTFTRDDKTLLGYPAEALATHLARLDVDAVGINCSGGP
                     AQVLRLISIVRQLVPDVPLSAAPNAGWPEQMAGGRVMYPATPDYFADYTRAFVEAGVN
                     IVGGCCGTTAAHVRAMRQALDTPGHSSLSMPEVRIVNRTERRVATGGEPTRLAQHLAA
                     KQLILTVEMDPPKGVAAERLLAGARMLREAGANFLNIADSPLARMRMSAWAAAYLVQK
                     DVGLEAVLHFPTRGRNLLRIQGDLLAAHAMGIRNLFVAMGDPTRIGDYPEAMDSYDIV
                     PTGLIELIKKQFNQGLDKAGLSIDQPTTFVVGCALSLTPKDIDAELRLLRKKVEHGAD
                     FALTQPVFDPEAALAFLEAYERAYGEPPPPILAGIKPLYNSRNAEFLHHEVPGIIIPE
                     ALRERMRLAEKPQAEGVHIAREIAAALQPHVRGFYFMPAFGRYDLVADVLDMFHAS"
     gene            complement(11952..>12851)
                     /locus_tag="PK829_01175"
     CDS             complement(11952..>12851)
                     /locus_tag="PK829_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39853.1"
                     /translation="YGAVVLLEDDLLVSARFHAYARQALAAYGDDPRLAGLSLNSPWF
                     NGFTHQPFVPLPDDGDVTFLQLSTPHGQVYTAAQWAAFRAWLAAATPATGAAAVHDLL
                     VALPEDDWLGTKARYLATTNRYYVYPRESLTTATGEPGTHFAQATSFFQVPLQERRRD
                     FRCLPFDAAVAVYDGFYELLPERLNRMTDRLHDCDYTIDLYATKPARRLTTDFVLTTR
                     PCRVTAATFGRALWPLEANVIAGVPGRGINLARREDVMMSDDAALAAQHANDAYFARY
                     RQPGRRRRWRGAWAYRRYRRWLD"
BASE COUNT         2240 a         4237 c         4110 g         2264 t
ORIGIN      
        1 agaagtacgg aggataattg acatgccctt gactgtggca gagaaagccg agttgatcga
       61 caactttgcg acgcacgacg gcgacactgg ctcgccggag gtgcagatcg cgctgatgga
      121 taagcgcatt cgccagctcc aggaacacct gaatactcac aagcacgatg aaagctcgcg
      181 ccgcgggttg cttaagctgg ttggcaagcg ccgccgcatg ttggcctatt tgcgcaagga
      241 acaccccgac cgctaccgta ctattcttgg acggctcggt ctgcgtcgct aaatctggaa
      301 atatgttggg aagggcgaag ggtgcttcgc ccttttctta ggagcaggca atcggccggg
      361 tttaggaatt gggtattgtc gcttacccta atcggtggcg gtaagagcca atccccagtc
      421 cctaacccca ttctgctccc cgtaattgga atcgggatcg atcatcaatt gtcgttacga
      481 ccgacagcgc atccgcacga aaagcggctg tctcgccgac ggcccgtctt gccgttgccg
      541 gttgcctgag cgccggcgat cgttctctgt gtatgggcgg actggctgtc ggcccgtgtt
      601 atcctgaagg aaagtagaat gaattcggaa tctgtatttt caaccacggt gggccgccac
      661 aagctcacca tcgccacggg taagctggcc gagcaggccg gcggagccgt cacgctccag
      721 gtgggcgaat cggtgttgct ggccacggcc acgatgtcga agaacatccg cgaagggcta
      781 gacttcttcc cgctcagcgt cgacttcgaa gagaaaatgt acgccgcggg ccgcatcccc
      841 ggcggcttct tccgccgcga gggtaagccg accacggagg ccgtgctgac cagccgcctg
      901 accgaccggc cgctgcggcc gctcttcccc gacggcatgc gcaacgaggt gcaggtcatc
      961 gtcaccaccc tgtcgtctga cagcatccat ctgctagaca tcatgtcggt caacgccgcc
     1021 agcgcggccc tgaccatctc cgacgtgccc tggggcggcc ccatcggcgc ggtccgtgtc
     1081 ggctacattg atggccaatt cgtcgttgac ccaaccgtcg aggagatggc tgaaagtcag
     1141 cttgacctgc gtatggctgg ctcgcgtgac gccatcatca tggtcgaagc ggcggccaac
     1201 gaagtgcccg aagagctgat ggttaaggcc ctggcctttg gccacgaggc gctgcaaccc
     1261 atcatcaccc tgttggaaga gatgcgcgcc gccgtgggca aagagaagcg cgagccgacc
     1321 atcgtcgtct ctgacgcggc attggctgag atgaccaagg gccgcatcgg cggccgcgtc
     1381 gccgaagtga tcgcctcgac gacggagcgt gaggcccgca acgaggccct cgacgccctg
     1441 cgcgatgagg tcgtcgatag cttcctggcc gacgacgaga cgaccgaccc caaggccgtg
     1501 cgcgaggtct ttgccgacga gatcaagaag gtcgtgcgcg atcgcatcct ttacgacggc
     1561 gtccgcccgg acggccgcga ctataccacc atccggccgc tgtcgtccga ggtgggtctt
     1621 agcccgcgcg cccacggctc cggcctgttc cagcgtggcc agacgcaggt gctatccatc
     1681 gtcgccctgg gcacgccccg cgaggcgcag aagctcgatg gcctgtcgcc cgaagacagc
     1741 cgccgctaca tgcaccacta caacttcccg cccttctcca ccggcgagac gtggcccatg
     1801 cgcggcccgc gtcgccgcga gatcggccac ggcgcgctgg ctgaaaatgc cctgttgcca
     1861 atgatcccgt cggaagacga gttcccctac actatccgcg tcgtctccga agtgctgtct
     1921 tccaacggca gcaccagcca ggccagcgtc tgcgcctcca gcctggcgct gatggactgc
     1981 ggcgtgccta tcaagcggcc ggtcgccggc gtggccatgg gcctggtgac tgatggccat
     2041 aagtacgctg tcctgaccga catccagggc atggaagacc atctcggcga catggatttc
     2101 aaggtcgccg gcacgagcga gggcatcacc gccctgcaga tggacatcaa gatcaccggc
     2161 ctggacatgg acatcatgac cagggcgctg gagcaggccc gcgtcggccg cctccagatt
     2221 ctggacagca tgctggccac catcgccgcg ccgcgcgagc agctcagccc ctacgcgccg
     2281 cggatgctga cgatcaaggt tgaccctgag aagatcggcg ccatcatcgg caagggtggg
     2341 gcgaccatcc gcgggctgga agaggaattc gaggtctcgg tcgacatcca ggacgacggc
     2401 acggtctttg ttgccggcgt cgatggcctg aaagccgagg cggcgctgga gcgcatccac
     2461 gccctgtccc acgacccgga gccgggcgag gtcttcaccg gcaagatcgt gcgtattacc
     2521 gacttcggcg tctttgtcga gttgacgccg ggccaggacg gcatggtcca catctcccag
     2581 ttggcttccg agcacctgca gagggtcgag gacgccgtgc gcatgggcga tgaggtcatg
     2641 gtcatggtga ccgacgtcag cccggagggc aagattcgcc tgtctcgccg ggccgtgctg
     2701 gagggctgga gtctcgacga ggcccgtgcc caggacgccg cccgcaagag cagcggcggc
     2761 ccgcgcagca acggcggcgg tggccgcgac cgcggcggcg accgccgtgg cggtggcgac
     2821 cgcggtggac gtccgccgcg tcgctagaac tggcttgacg gttcgctgac gagtgtacca
     2881 ggtaggggcg ggtctcagac ccgcccctac cataccttgt gggcgagagg catgtctccg
     2941 ctgccgcgag tgggcgcggg cggcctctac gccttatcat agtggcgggc gagatggccc
     3001 gcatgaagga gcgccgacga tgagacgttt tagctggttg ctcattccct tgctgtggtt
     3061 gctggccgcc tgtggctccg gcgcagacca gtttagcaac cccacggccg tggccgggcc
     3121 gggcgaggac agccccccca cggcgaccgt cagggcgacc acggcggccg acagcaccgc
     3181 tgtcccggcg gccaacgccg acgaccccac acaggcccgc gagagagatt ggaagcaggg
     3241 cgatctggaa gaccccgccg ttaccattat cgagtacggc gactttcagt gaccgggctg
     3301 cgcagggttc gcccccctgc taaagcgtct ggtagacgct taccccgatg accttcaaat
     3361 catttaccgg catttcccgc tgaccagcat ccaccctaac gcccagaagg cagccgaggc
     3421 ggccgaggcg gccggcgctc agggtaagtt ctgggagtat catgacgccc tgttcgagcg
     3481 ccagaatgac tggagcgcgc tgagcgcagc agaggcgcga cagtttttca ttgacctggc
     3541 cgtggaactg gggctggatg gcaacgcctt tacggccgcg ctggacaatg gcgactttgc
     3601 cgagtacgtg tccggactgg agagtgaggc ggtggcgctg ggcctgcccg gcacgccgtc
     3661 ggccgttgtc aacgggcagg tcattcctag ttcaaacttg cccttcgatt ttgaaacctg
     3721 ggaggctttt gtgaaagacg aaatcaagct gcaacaactg gccgcgcgcc aatacaaggc
     3781 cgcgccgccg atgacgatca acaccgacgc ccgctatttg gcccatgttg agatggccaa
     3841 tggcggcgtc ttcaccatcg aactgctgcc caagtctgcc ccgcagacgg tcaacagctt
     3901 cgtctttctg gcccgcgagg gttggttcga cggcgtgacc ttccaccgcg tgctgcccgg
     3961 ctttgtggct cagacgggcg accccagcgg caccggccgc ggcggccccg gctacatgat
     4021 ccccaacgag atcgatagca gcctgtcgca taacgaggcg ggtgtcgtcg ctatggccaa
     4081 tgccggcccg aacaccaacg gcagccagtg gtacatcacc tacggtgatg tcagccagct
     4141 agacggctca tataccatct ttggccgggt catcgaggga atggatgtgg tcgaggccat
     4201 cacgccacgc gatccatcgc gcaaccccac cgcgccggcc ggcgatgcca ttgcccacgt
     4261 caccattgag gaaattcagc cctaaccggc tgctcttcat atgaccggcc tggcgatgag
     4321 aaccactgtg gttctcgctg ccaggccggc ccatctccca ttgactgtgg cgaacactca
     4381 gcggagaggg gtagggtatg acgtacagat tccagttgcc ggctgagccg ttggtggaac
     4441 gcaatccctt tcgccagggc gagcggcgat cgtcgctgac caactttgcc gacgtacgtt
     4501 cctgcctgcc ggtgccggtc tttccccagc aaccgggctg ggagcggctc tactggacgg
     4561 cttgggagca tctgtggtcc tgtctgcgcg cgccggcgac taactcgcgc ctgatcgccc
     4621 cctatctaca gccatccggt gatcaacctg tcgagatggc cccggccgct ttcgtgagcc
     4681 agttggctgg ctacataccc ggttcaacgg cgctgatcga tcctctcgat aacttctacg
     4741 cccggcagca cgacactggc ttcatctgcc gcgcctttaa cccagacgat ggtcgcgact
     4801 tccaccagcc ctatgagccg aacagcaccg gcccggccct gctggcctgg ggcgagtggc
     4861 ggcgctttcg cctgacgggc gacgcgtcgc gcattgctga cgccttttgg cctctgttgg
     4921 cctaccatcg ctggtgccgt gccaatcgca cctggcccga tggcctgtac tgggcgacgg
     4981 cgtacagcag tggcctgacc aaccagccgc gcgtgcccgg cggccgcacc caccatgccc
     5041 actggacgtg ggtcgatgcc tcagcgcagg ccacgctgga cgcggccctg ctggaacgta
     5101 tggccgtcct tttgggcgag caagccctgg ccgatgaggt ctccgccgag cgtgtaatgc
     5161 tcatccagcg cttcaacgcc accctctgga acgaaaacca cagcttctac caggatgctg
     5221 gccctgatgg ccgcttcagc ccggtgcgca gcatcgccgc ctattgggcc ttgctcgacg
     5281 gtcacctggt gcccaaggag cggctgacgc cttttatcca gcatctgcgc gacacctggt
     5341 cattccgaac gcccatggtg ctgccgagca tggccgccga cagcgaagcc tacaatgctc
     5401 gcaccggcaa cggttggcgc ggcggcgtct ggccaacact aacctacatg gtcttgcgcg
     5461 gcctgggtct gaatgagcag catgtcctgg cccacaagtt ggccctcacc cacctggatg
     5521 tggtcggaca gctgttcgag acgaccggcc gcctgtggag ccactacgcg cccgaagacc
     5581 tcagccccgg cgacccgtcc gaggaggatg ccgccggcca gacggcggcg gcgatcatcg
     5641 ccatgctcct ggagaacatc gtcggcgtct gggtcgattg gccgttgcgg caggtgacgt
     5701 ggcgacgcca ccttcagggg gccgaagagt atggcgtgcg acaactgccc ctgggcaacg
     5761 agggcacgtt ggacctaatg agcgccggca acctggtgcg cgtgcgcacg gacgcgccgc
     5821 tgacgctggt cttgcacaac ggttcggagg ttgtgcggac ggccgtgccc gccggcgcgt
     5881 tcgagatcgc cctggactaa gcccgagtcc cttccggtat gttcgccttc accgtcgcca
     5941 ctatcaacct gcacaaccgg gccgaccgct ggctggcgcg ccgccacctg atggtcggcc
     6001 aactcatgga cgttcagccg gacatcgttg ccttgcaaga gatcagtctg accattggcc
     6061 aggccacctg gttagcgcgg cagatcaaca ctcgtctgac cggcaaatcc aaccagccct
     6121 accgcgtcat ccaggcgccc cgcgccagcg cccgccatcg cttcgagggt gtcggcgtgc
     6181 tgacgcgctt gccggtcatc taccacgacg tgttgccgct ggggttcgac ggccgcatcg
     6241 ccctgcgcgt taacgtcgag ttgcccgccg gggcggccgg ctcgcgccgg caatcgcttg
     6301 atttcgtcag cgcccacctg catcatgtcg ccaccgaccg cgacgcccgg ctggaacagg
     6361 ccatgatcct ggtcggctgg ctcaacgata agcggcgcgt gcccttgcag gtcatcgccg
     6421 gcgacttcaa cgaagcgccc gacggcccgg ccatcgcctt tatgcgccag tcgtatcgct
     6481 cggtctacgc cgaggtgaaa gggcgcgacc cgttggcgac ctttccgacc aatctggtgc
     6541 gcccgccaac ggactgggcc gcctgtcttg attacgtctt tgtctcgccg gccgtgtata
     6601 aagtaacgag ggcggcgatt ttcctggaca agcccgcgtc cgacgacgat acgctctatc
     6661 cgtccgacca cgtgggtttg ctagtaggcc ttgaggtatg acttgtcggc agaagaacga
     6721 cacgacctga tctccatcgt tgcgccggtg tacaacgagg agggtgttct ggacgcgctc
     6781 tatgagcgcg tgcgcgccgt gttggacgga gcgggagaag tgtgggagtt ggtgttggtc
     6841 aacgacggca gccgcgaccg ctcggccgac gtcatcgccc ggctgcacgc ccaggacccg
     6901 cgcgtgaagg ggctgagctt ctcgcgcaac tttggcttcc agatcgcggc tacggccggg
     6961 ctggactacg cccgcggcga cgccgtcatc ctgaccgacg ccgacttgca ggacccgcca
     7021 gaggtctacc cggcgctgct ggcccagtgg cgcgctggct acgacgtcgt ctatggcatc
     7081 cgcgccagcc gcgaggggga gacgtggttc aagctcgcca cggccaagct cttctatcgt
     7141 ctcatccacc gcatcaccag cattaacatc ccgttggaca ctggcgactt tcgtctgttg
     7201 gatcggcgcg tcatcaacgc cctgcgcggc atgaacgaac gcaaccgctt cctgcggggc
     7261 atggtgccct gggtcggctt ccggcagatc ggcgtggagt accaccgcga ggcgcgctat
     7321 gccggcgagg ccaagttcac cagcgtcaag aagatgttgc ccttcgccct ggacgctatc
     7381 actagctttt cctacttccc cttgcaactg gcgaccatcc tgggcttcgt catggcgacc
     7441 atcagcgccg tggccattct ggccgtcgtg ctggtgcgcc tgctggggcc gcacgagccg
     7501 ctgctgggcc aggcgacgac actggtggcg gtgctgttcc tgggcagcgt ccagctcatc
     7561 tgtctgggga ttatcggcga gtacctgggg agaatctacg acgaggtgaa ggggcggccg
     7621 ctctatctca tccagcagag ttggggcatc gacaacgcgc cgctagggcg ctttgccccg
     7681 cccacggggc cttccagcgg ggccgaacag aagcgggcct aaccgcctat ctctgacata
     7741 acacggttca aggggatcac gtcatcggcc aagccatggc cccacggctt ccaccaaatg
     7801 ccagcggtga tggttgctcg catctcttcg tcgtcagccc cggcgcgcat gggcgtcagc
     7861 agatccactt ccttctcgcg cagcagacat aggcgcagcc ggccatcggc cgtcagccgc
     7921 gcccggttgc acgacgcgca aaacggatgg gtgaccgagc taatgaagcc aatcgttccc
     7981 ggcgcgccgt ccagctggta gaggctggcc tcgccatcga gatgaccgtc gttgaccggt
     8041 cgcagcggcc ccaggtgggc ggcgatggtc gcccgcaact catcctcgct gaccacgccg
     8101 gccagctgga agtcagccac gtcgccaaag ggcatcatct cgatgaagcg cacctgccag
     8161 ggttggtgga gcgtcaggcg ggccagatcg accaggtcct cgcggtcgtt gtagttgcgg
     8221 acgatgaccg cgttgagctt gatttccagc ccggccgcct cggcggcacg aatgcccgcc
     8281 cagacctctt cgaccttgcc ccagcgggtc agcttcttga agcggtcggg gtcgagcgtg
     8341 tcgatgctga tgttgacccg ctgtagaccc gcctcggcca acgggcgggc cagccggtca
     8401 agcagcagcc cgttggtggt catggcgatc ttgtccacgc ccggcgtagc cgccatctgc
     8461 cgcaccaggt cgacaacgtt ggcgcgcacg gttggctcac caccggtcag ccggaactta
     8521 tggaagccca actcagcaaa gagacgcacc aggcggagca tctcctcgtc ctgcaacagc
     8581 tctgcgcgcg gccggaaggt catatcctcc ggcatgcagt agacacagcg caagttgcag
     8641 cggtcggtca gagagatgcg caggtagttg atgttcctgc caaagcgatc gattgacata
     8701 gtgtttgacg gtgcctgacc agcggcgcgg gttgccaggt agccacgata ctgtacctga
     8761 tttgccacgc tacggtcaag gccgaacaca ctatttttat cacaaacatc ataaaaagcc
     8821 agtaatggtt gattggcatg gccggcggtt gtctgcgggc tgccatttat gagggggcgt
     8881 tcagggtgta gagcaaacgg tgggtttgcg gcgggaacct cagcagcagg ggaagcgtta
     8941 tagggctata cttagagcca ttgtaggcca atgtgcatcc gggttcgccc ggatcaggag
     9001 tgtagatttg tacaaacagc cattactacc gggctggttg agtgccctct tgtcggccgc
     9061 ctttgtcttt tcggccgtgg cggtgctgtt ctttgcctat ttgaccgtcc agcacctgat
     9121 gagccggccg gcgacaatgg tcgatgccgc cggcgctgag ggtggtctgt cgcccgtggg
     9181 cggcgacctg ccaccggcgc aggtgatcga cggcgggact gcgccaacgc cgactccggc
     9241 cgtgccgacc gtggctgcct gggacggcga ctcgcgcgtg aacatcttgc tgatgggcat
     9301 cgaccggcgg ccgggcgaaa gcttcgtctc gcgcaccgat acgatgatga tcctgtcggt
     9361 cgatcctgaa accgattcag ccgtcattct ctccgtgccg cgcgatctct acgtacagat
     9421 tcccggcttc ggcgaagacc gcattaacac cgctctggtg ctgggggcgc gcaatggcga
     9481 ctatctcgac ggcgctgctc tggccatgca gacggtatcg tataacctca acatccccat
     9541 tcatcacttc gtgctggtgg acttcaacgc tttcgtccgc agcatcgacc tgctgggcgg
     9601 cgtcgacgtc gatgtgccct acgagatcaa cgacccggaa tatccagaca tggactatgg
     9661 ctacgatccg ctctacatcc cggccggttt gcagcacttc gacggagcga tggcgctgaa
     9721 gtatgcccgc acgcgccacg gcgacacgga cttcaaccgc gcttttcgcc aacagcaggt
     9781 gctcttcgct gcccggcaac aggtggtcaa gctgggcatt ggcgagatgc tgctgcgcgc
     9841 gccgtcgctc taccgcgagg tggaggaggg tatccgcacc gacctgagcc tggagcagat
     9901 gttgcggttg gccaagacga tcagcgatat cccgtcgggt aacatccgca acgacgtgct
     9961 cgactacgat tacgtcagca gctaccgcac gcccgggggg gcctccgtgt tgctgctgcg
    10021 gccggagacg gcggtacctc tgatcgagag cctcttcggc cagtaatggg gtacaatcac
    10081 cccacgatga atcgccagga attcaaacaa cgacttgagc aaggccccgt gctgatggac
    10141 ggggcctttg gcacggtcct gcacggccgc ggtatcccta tcgaccagtc cttcgatgcc
    10201 gtgaacctca ctaatcccgc gctggtggcc gaaatccacc gcggctacat cgacgccggc
    10261 tccgacctga tcgagaccaa caccttcggc gctaaccatt acaagctggc cgagcacggc
    10321 ctgcaagacc aggtagcggc gatcaaccag gccgccgtgg ccattgcccg gcgggtgatt
    10381 gccggctcat tccgagaggt tttgctggcc gggtccattg ggccgctggg cgtgcgcctg
    10441 gcgccgctcg gccgcgtgcc catgaccgag gccgaggctg ccttccgtga gcaaattgag
    10501 gcgctggctg cggggccggc tggcgttgac ctgctcatca tcgagacgat gtcggacatt
    10561 aaagagatcg aggcggcggt caacgctgcg cgggccgtcg ctccggagct gcctgtcatc
    10621 gcccagatga ccttcactcg cgacgacaag acgctgctcg gctacccggc cgaagcgctg
    10681 gccacgcacc tggcccggct ggatgtcgat gccgtgggca tcaactgtag cggcggcccg
    10741 gcccaggttt tgcggctcat cagcatcgtg cgccagctgg tgcccgacgt gcccctctcg
    10801 gccgcgccca acgccggctg gccagagcag atggccggcg gccgggtgat gtatccggcc
    10861 acacccgact acttcgccga ctatacccgc gccttcgtcg aggccggcgt caatatcgtc
    10921 ggcggttgct gcggcacgac ggcggcccac gtccgggcca tgcgccaggc gctggacacg
    10981 ccaggccata gcagcctcag catgcctgaa gtgcgaatcg ttaaccggac ggaacggcgc
    11041 gtggccactg gcggcgagcc gacccgtctg gcccagcacc tggccgcgaa gcaactgatt
    11101 ctgacagtgg aaatggatcc gcccaagggc gtggccgccg agcggctgct ggccggcgcg
    11161 cgcatgttgc gcgaggccgg ggccaacttt ctcaacatcg ccgatagccc gctggcgcgg
    11221 atgcgcatga gcgcctgggc ggcggcctac ctggtgcaga aggatgtcgg gctggaagcc
    11281 gtactccact tccccacgcg cggccgcaac ctgctgcgca ttcagggcga cttgctggcc
    11341 gcccacgcca tgggtattcg caacctcttc gtggccatgg gcgaccccac ccgcattggc
    11401 gactaccccg aagcgatgga ctcctacgac atcgtgccca ctggcctgat cgagctgatc
    11461 aagaagcaat tcaaccaggg gctagacaag gccggcctgt cgatcgacca gccgaccacg
    11521 ttcgtcgtcg gctgcgcgct tagcctgacg cccaaggaca tcgacgccga gttgcgccta
    11581 ttgcgcaaga aggtcgagca cggggccgac ttcgccctga cacagccagt gttcgacccc
    11641 gaggcggcgc tggccttcct ggaagcgtat gagcgcgcct atggcgaacc gccgccgccc
    11701 atcctggccg gcatcaagcc gctctataac agccgcaacg ccgagttcct gcaccacgaa
    11761 gtgccgggca tcatcatccc cgaagcgctg cgcgagcgga tgcggctggc cgagaagccc
    11821 caggccgagg gcgtccacat cgcccgcgag atcgccgcgg cgctgcagcc gcacgtgcgc
    11881 ggcttctact tcatgcccgc tttcggccgc tacgacctgg tggccgacgt gctggacatg
    11941 ttccacgcaa gctagtccag ccagcggcgg tagcgccggt aagcccacgc gccgcgccag
    12001 cgccgccgcc ggcctggctg ccggtagcgg gcgaagtagg cgtcgttggc gtgctgtgcc
    12061 gccagggctg cgtcgtcgct catcatcacg tcctcccgcc gcgccaggtt gatgccccgc
    12121 ccgggcacgc cagcgatcac attggcctcc aacggccata gggcgcggcc gaaggtagcc
    12181 gcagtgacgc ggcaggggcg cgtcgtcagc acgaagtcag tcgtcaaccg gcgcgccggc
    12241 ttcgtggcgt agagatcgat ggtgtagtcg cagtcatgca ggcggtcagt catgcggttc
    12301 agtcgctctg gcagcagttc gtagaagcca tcgtagacgg ccacagccgc gtcgaacggc
    12361 aggcagcgga aatcgcgccg ccgctcttgc aagggcacct ggaagaaact cgtcgcttgc
    12421 gcgaagtggg ttcccggctc gccggtggcg gtggtcagcg actcgcgtgg gtagacataa
    12481 tatcggtttg tagtggccag atagcgcgcc tttgtgccca gccagtcgtc ctccggcaga
    12541 gcgaccagga gatcatgcac cgctgctgcg cccgtcgccg gtgtggccgc ggccagccag
    12601 gcgcggaagg cggcccactg cgccgcggta tagacctggc cgtggggggt gctcagttgc
    12661 aggaaggtca catcgccgtc gtcgggtaag ggcacaaaag gctggtgggt gaagccgttg
    12721 aaccacgggc tgttgagcga caggccagcc agccgcgggt cgtcgccata ggccgccagc
    12781 gcctgtcgcg cgtaagcgtg aaagcgggcg gagacgagca gatcatcttc cagcagcacg
    12841 accgcgccat a
//
LOCUS       DAOSMQ010000017        12568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5007_length_12568_cov_4.734436, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000017 DAOSMQ010000000
VERSION     DAOSMQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5007_length_12568_cov_4.734436"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..650)
                     /locus_tag="PK829_01180"
     CDS             complement(12..650)
                     /locus_tag="PK829_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39854.1"
                     /translation="MRSQIRQAPLFLLGCLLALTLLLAGCGGAAGNATATMAPATTAG
                     GALATSTAPAAPDTTGAPSTTDTYPAPVTSEESLSAYPSEGEAAVPADSYPAPAAEEA
                     ILEPRFRIDQPVTAAMTTITGQAPPNLVLAVLDVTYSGVPLGQGRSDENGAFAIPVSG
                     LTVGNRIGLAIGELPEGMDIAQTAEAYFPHRGEGFQNLPNLGVFYDTTLVQP"
     gene            complement(766..2187)
                     /locus_tag="PK829_01185"
     CDS             complement(766..2187)
                     /locus_tag="PK829_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39855.1"
                     /translation="MFKKTFVLVLALLLVAVGVVSAQSGNLPGSGWWSGQQIQNVGTA
                     NASVLFTAYDDQGNDYACGTAKTAAPGQSVNFLTNTDCSVSAGFVGSAVVSADQPIAA
                     IVNVVNMPSGRAAGMYRGTDGADVANTIAFPLMKSNFFGRSTTLYIQNASSSPNNITV
                     NVKVGGANFSKTFNNVPANAMVLVGPADTNPAMNNNTYGSATVTGTGPLAGAALEYQT
                     AVALGSNLHAYAAFSPQDFGAKAYCPLIRSNYARLNTGIQAQNVSTSAQAIRVTYSYR
                     MNNTGAVQTKVVTSGSIAPNAAATFSTWTDLPSGAVGGATVEGVGGGNIAVIVNDETF
                     TLNPNRVTAYTCFADSAKTNKVALPLYKEYYLGDTSGVQVQNVGTAAANISVTYYPPG
                     NPAGVTFTQNNIAAGASATFYNVSGGTASSGNAASLLGTFGGAVVNANQPVVAIVNEE
                     PGTKAGARPASTQDAKLYEGFNQ"
     gene            complement(2300..3742)
                     /locus_tag="PK829_01190"
     CDS             complement(2300..3742)
                     /locus_tag="PK829_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOU39856.1"
                     /translation="MNTLQRLLSNTFLAFASSVLVKAGNALLFILIGRQLGPAASGSF
                     SLATTYFTFVFGLSALGLHEIMVREVPARREESGRYLVNYVSLRVVLSAVVYGLLVLG
                     LWRLSPYTAATTQVIAILCLAAVPEAIFSMSQSLFEAHERLLTPLVAATVSTAVKLGA
                     GFWLLRSGADVAQIAWVVPLASVLGVAVFAGGLARLFRQAGPQLPARLDPTFLRHHLG
                     YMPSFFIIQMFSLLDYQADILLISLMLSENDVGLYAAAQTILLGFNLMPVAVRTALYP
                     VMSRYYYQAPDKLAVLNDKISRYLLAVILPVATGVTLLAGPIIALVYGPAFGPATPVL
                     QVSIWAIVFLFLNVPHSRLALIYNRQRDAAILLSLTTGLNLALNLVLIPRAGILGAAV
                     ARLLSSLALFLVFYVYAQTRLTRVSLLASPVRPLLATAAMAAAVWPLRDQPLAIPIAA
                     GVLVYGIAALLLDLVPQDDRVYWREVLRRR"
     gene            complement(3717..4478)
                     /locus_tag="PK829_01195"
     CDS             complement(3717..4478)
                     /locus_tag="PK829_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU39857.1"
                     /translation="MRLGREGQLALLPRWVSEKLDVHLWETNRLLAQAQAELPPGARL
                     LDAGSGEGQYRDTFKHLRYVGVDLAVGDQTWDYTGLDAQGDLSHLPFADDSFDAAVCF
                     QVLEHVNEPALVIDEIGRVLRPGGRFYLSAPMAWHQHQKPHDFFRYTSYGFTHLLEKA
                     GLRVVEMRPWGGYFWFLSFNFQMMHDRLFPRGDNDLAYLLKLPLILPVYLVFFLLLPL
                     LLFYLDRLDRVKDHTLGWACIAVKPPADEHAPAPA"
     gene            4616..5326
                     /locus_tag="PK829_01200"
     CDS             4616..5326
                     /locus_tag="PK829_01200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOU39858.1"
                     /translation="MQTNSNTSTNTHPWSPSLKARLYGLARPAEEWLLRAVYTSRFYQ
                     WSKARAAAPTLATRFDLYQYVIDAHGLGGTIDYLEFGVYHGESLRWWAAHNSAPASRF
                     YGFDVFTGLPDAWVGLPAGTFTTHGQVPTFDDPRVELEIGLFQDTLGPFVDGHALDRR
                     TVIHLDADIYTSTLYVLTTLAPRLRPGDVLLFDELGSAYGVSHEFRALEDFASSYRCQ
                     YRLLAGTRRYLQAAIELL"
     gene            complement(5334..6320)
                     /locus_tag="PK829_01205"
     CDS             complement(5334..6320)
                     /locus_tag="PK829_01205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU39859.1"
                     /translation="MNADAIALVIVAWNQLDKTLDCLASVAALRHAPGRVVLVDNGSA
                     PPLAAAVAERFPAVEGLRLPRNLGFAGGYNAGLRHVLAGEPAAVLLLNNDTLLAPDAL
                     DQLIAQMEEERLTPGRQDARAPGGDGRGEFIGLVTAKIYYATEPERIWTVGANLNVFL
                     DLKDGGQGQRDVGQWATPRDVDFAPFCGILIRREVFATVGLLDEAFFLYYEDMDYCRR
                     ARLAGWRIRLCPSAHILHAVSASSGGRDSPLERYWMAQSSGRYFRKHGRGLRLLLIVP
                     FRLASAVKMTARLLLAGKTTALRAYWRGLWLGWRTGRATTPPPAWARGVSAP"
     gene            complement(6317..7288)
                     /locus_tag="PK829_01210"
     CDS             complement(6317..7288)
                     /locus_tag="PK829_01210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU39860.1"
                     /translation="MRASVIIPSLNAPTLSRALQAIAAGTELPDEIIVVGRDEAGALK
                     AFPPPSESGPTHVPLPSESGPTRVPPPSGRGLGGGIVRFIDTGAPVCAARARNLGLAA
                     ATGELLLLLDADCIPHPGWLAAHRARHVAGEQVVGGAVALAGSNYWAQSDNVSMFHEF
                     IPQRPAGARVLLPTLNLSLRREVFAAVGGMDESFPGAAAEDADWTIRLRRAGYRLWFD
                     PAAVVAHSPARTTRADVARHWRNLGHNAVRVRLRYPEEFGTPAGARRAAWWRLLSPLI
                     ATRITAGVYAKRALWRYWPSLPVVYASKVLYCCGAARAIDSGFATRP"
     gene            complement(7290..8558)
                     /locus_tag="PK829_01215"
     CDS             complement(7290..8558)
                     /locus_tag="PK829_01215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOU39861.1"
                     /translation="METPTASLQLKPGRDKPVHNRHPWVFSGAVGRLHGDPQPGDLIT
                     ITDERDNPLATATFNPRSQIVARILSWDPAEPIDDAFWGRRLERAIAARAALGLSSDA
                     GAALVSPAPRLPGSPVPASPTTAYRLVNAEADGLPGLIVDRYGDHLVMQCLTLGIDRR
                     KAMLTAQLAELLRPAGILERSDVDVRGKEGLRLLIEPRHGQPPPPEMTIHENGHAFLV
                     DVWRGHKTGFYLDQRDNRAAVGRPALLAGREVLNVFAYTGAFGVYAAAAGAARITQVD
                     SSAPALEVAERNMALNGFDRPDDELIAGDAFEVLRYYRETGRQFDVVVLDPPKFAHSQ
                     GQVERACRGYKDLNWLALRLLRPGGLLATFSCSGLVSADLFQKVVFGAAVDAGRDVQI
                     LQHLGQAADHPVLLSFPESAYLKGLLGRVW"
     gene            complement(8665..9606)
                     /locus_tag="PK829_01220"
     CDS             complement(8665..9606)
                     /locus_tag="PK829_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU39862.1"
                     /translation="MAPMHSDPLRLRSDLLLLLAAFIWGFAFVAQRAGVEHIGPMAFT
                     AIRFFIGGLVLLPIILAGERQGRPAAAGAGRRTLLSKHHLASILVGVALCAGATLQQL
                     GVARTTAGKSGFITSLYVVIVPLLGLALGRRAPAATWVGVLLAAAGLYFLTMQGSFRM
                     GLGDFLVLLGAFAWAVHMLLLGHFSPGRDPVKLAFTQFMVCAALSGLAALLLETTTPA
                     DVRAAALPILYTGVLSVGVGYTLQVAGQRHAPPADAAILMSMESVFAVLGGWLLLSEQ
                     LTARALLGCALMLAGILVSQLWPARARAAAATLPAEH"
     gene            complement(9631..10488)
                     /locus_tag="PK829_01225"
     CDS             complement(9631..10488)
                     /locus_tag="PK829_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU39863.1"
                     /translation="MLHRLIPTNGVTLHTVLDGPEDGPVVLLLHGFPEFWYGWRQQIP
                     ALAAAGFRVLAPDQRGYNLSDRPRALSAYSLDALANDALGLIDASGRERVYLAGHDWG
                     AMVAWWVALIAPHRVQRLAILNVPHPAVMLNHLTTDPAQMSRSLYAAFFQLPGLPERL
                     MEANDWRLLARTLRDTSLPGTFSDADLEEYRRAWSRPRAMTTMLNWYRALVRRPPAEV
                     SSTRVVVPTTILWGMKDFALRAVMAEESAALCDDGELLAFPDNTHWLQHEAAAAVNEA
                     LISRFRPQP"
     gene            complement(10526..11026)
                     /locus_tag="PK829_01230"
     CDS             complement(10526..11026)
                     /locus_tag="PK829_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOU39864.1"
                     /translation="MRRVGILTISDRAAAGDYADESGPAAAAAVTAATGWPIERQAII
                     PDDFDAIVRALRQWCDDGLELLLTSGGTGFAPRDVTPEATRVVIERDAPGIAEALRAE
                     SLHVTRHAMLSRGVAGLRGQTLIINLPGNPKAVRENLDVLLPVLPHALDLLTARPESG
                     QGHREV"
     gene            complement(11023..>12568)
                     /locus_tag="PK829_01235"
     CDS             complement(11023..>12568)
                     /locus_tag="PK829_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU39865.1"
                     /translation="LDKLLVLPGFEFTATFGFHILGIFPPETPVSYLEHLLLTLRVPP
                     ALLREGSSTVGATSDVLTAYHVINEAGGLVIAAHVNSGNGVMMRGLDFGGQTRVAYTQ
                     DPNVHALEVTDLEKRGNQTTRRFFDGSKPEYPRPMRCIQGSDAHRLIRESATSPYLGV
                     GDRVTEILLEEVSFAALAGVIRGNDYSLTRPYRGTAKAVDFVQLAREEGESLVQAFHP
                     TINQRGGHLDNILRDICAMANTNGGTIYVGVPTDPKAKAEGVREPAKAIEALRTTLAK
                     RFSPKPPVQIDSLPTQGTTVVRVTVTPGPDAPYAIDSNLFYVRDETETTLAVRDEIVQ
                     LVERGLERRAGQIAAPAEEPARPTPTPVAAAPAPRADRRRSETARATPAEKPPPASAP
                     STNGKRERPRTGVELVESQERDGTIYHTLRDLRSGNLINNVTRSSARRLWHYAITQAE
                     TTPPNLGSVQWRQNVAVLNRRPKGNSTLYDLAVRADDGVHVYYGVTDSGLSEEWLALV
                     EEPRRE"
BASE COUNT         2035 a         4123 c         4520 g         1890 t
ORIGIN      
        1 ggcgtcgtgc gttagggctg caccagcgtc gtgtcgtaga agacgcccag gttgggcagg
       61 ttctggaagc cctcgccccg gtgggggaag taggcctcgg ccgtctgggc gatgtccata
      121 ccctccggca actcgccgat ggccaggcca atgcggttgc ccacggtcag gccgctgacg
      181 ggaatggcga aggcgccatt ctcatcgctg cggccctggc cgagcggcac gccgctgtag
      241 gtcacgtcaa gcacggccag caccaggttg ggcggcgcct ggccggtgat ggtggtcatc
      301 gccgcggtga ccggttggtc gatgcggaag cgcggctcca ggatggcctc ctcggccgcg
      361 ggcgcggggt agctgtcggc cggcacggcc gcctcgccct cagacgggta ggcgctcagg
      421 ctctcctcgc tggtcactgg ggcggggtag gtgtccgttg tagacggagc gccggtggtg
      481 tcgggggcgg ccggggcggt gctggtggcc agcgcgccgc cggcggtcgt ggccggggcc
      541 atggtcgcgg tggcattgcc ggccgcgccg ccgcagccgg cgagcagcag agtcagggcc
      601 aacaggcagc ccagaaggaa cagcggtgct tgtctgattt gtgaacgcat agtttcgatt
      661 ctagcctaaa gggggcaaga aaagaagcca cctggtgggg tggcttcttt tcttctcagg
      721 tggccgaaca cggccgccca tgttcaaccg gatgcctagt ggttcttact gattgaaacc
      781 ctcgtacaac ttggcgtcct gcgtggaggc gggacgcgcg ccggccttgg tgcccggctc
      841 ttcgttgacg atggccacga cgggctggtt cgcattgaca accgcgccgc cgaacgtgcc
      901 cagtagcgag gccgcgttac cggacgacgc cgtgccgccg gaaacattgt agaaggtcgc
      961 gctggcgcct gcggcgatgt tgttctgcgt gaacgtcaca cccgcgggat tgcccggcgg
     1021 gtagtaggtc acgctgatat tggccgccgc cgtgcccacg ttctgtacct ggacgccaga
     1081 ggtatcgccc aggtagtact ccttatagag aggcagggcg accttgttgg tcttggccga
     1141 atccgcgaag caggtgtagg ccgtcacgcg attcgggttc agggtgaagg tttcatcgtt
     1201 gacgatgacg gcgatattgc caccgcccac gccctcgacc gtcgcgccgc ccacagcgcc
     1261 cgacggcaga tccgtccagg tgctgaacgt ggctgccgcg ttgggggcga tggagccgct
     1321 cgtgacgacc ttggtctgca ctgctccggt gttgttcatc cggtagctgt aggtcacacg
     1381 gatcgcctga gccgaggtgg agacgttctg ggcctggatg cctgtgttaa ggcgggcgta
     1441 gttgctccga atcagcgggc agtaggcctt ggcaccaaag tcctgcgggc tgaaggcggc
     1501 ataggcatgc aggttgctac ccagggcgac agcggtctgg tactccaacg ccgcgccggc
     1561 caacgggccg gtgccggtaa cggtggcgct accataggtg ttgttgttca ttgccggatt
     1621 ggtgtcggcc gggccgacca gcaccatggc attggccgga acgttgttga acgtcttgct
     1681 gaagttcgcg ccgccgacct tcacattgac agtgatgttg ttcgggctgc tgctggcgtt
     1741 ctggatatag agcgtcgtcg agcgaccgaa gaagttgctt ttcatcagcg ggaaagcgat
     1801 cgtgttggcc acgtcggcgc catcggtgcc gcggtacatg ccggcggcgc gaccgctggg
     1861 catgttgacg acgttgacga tggcggcgat aggctgatcg gcgctgacaa cagccgagcc
     1921 gacgaagccg gccgacacgg agcagtcggt gttggtcagg aagttaaccg attggccggg
     1981 cgcggccgtc ttggcggtgc cacaggcgta atcgttgccc tggtcatcat aggcggtgaa
     2041 caggaccgac gcattggccg taccgacgtt ctgaatctgc tgcccagacc accagccgga
     2101 acccggcagg ttgccggact gggccgacac gacgccgacc gcgaccagca acagggccaa
     2161 aacaagaacg aaggtcttct taaacatagt tgtctccttg gaacctaaaa catccagtct
     2221 tgcgaacact agcattctag gcgatccgcc cgccaaaaca atcgcccgtt tggagtattt
     2281 gggggagtaa tctgtccgat tagcgccggc ggaggacttc gcgccagtaa acgcggtcgt
     2341 cttggggcac gagatcgagc agtagggcgg cgatgccgta gacgaggacg ccggcggcga
     2401 tggggatggc cagtggttgg tcgcgcagcg gccacaccgc cgcggccatc gccgccgtgg
     2461 ccagcagcgg ccggacgggc gaggccagca ggctgacccg cgtgaggcgc gtctgcgcat
     2521 agacgtagaa gaccaggaac agggccaggc ttgacagcag ccgggccacg gccgcgccca
     2581 ggatgcccgc ccgtgggatg agcacgaggt tcagagcgag gttcagcccc gtggtgaggc
     2641 tcagcaggat ggccgcgtcg cgctgccggt tgtagatcag cgccagccgc gaatggggca
     2701 cattcaggaa caggaaaacg atggcccaga tagagacttg tagcaccggc gtcgccggcc
     2761 caaacgccgg gccatagacc agggcgatga tcggcccggc cagcagcgtc acgcctgtgg
     2821 ctaccggcag gatgacggcc agcaggtagc ggctgatctt gtcgttgagc acggccagtt
     2881 tgtcgggagc ctggtagtag tagcggctca tcaccgggta gagtgcggtg cgcacggcca
     2941 cgggcatcag gttgaagccc agcaggatgg tctgcgccgc ggcgtagagg ccaacgtcgt
     3001 tctcgctcag catcagcgag atgagcagga tgtcggcctg atagtcgagc agggagaaca
     3061 tctggatgat gaagaagctg ggcatataac ccaggtggtg gcgcaggaag gtcgggtcga
     3121 gccgcgccgg cagctgtggc cccgcctgcc ggaaaagccg cgccagccca ccggcaaaga
     3181 cggccacgcc cagcaccgac gccagcggta ccacccaggc gatctgggcc acgtcggccc
     3241 cggagcgcaa cagccagaag cccgcgccta gcttgaccgc cgtgctgact gtcgctgcca
     3301 ccagcggcgt cagcaggcgc tcgtgagcct cgaagagcga ctgcgacatg ctgaagatcg
     3361 cttcgggcac ggctgccagg cacaggatgg cgatgacttg cgtcgtggcc gcggtgtagg
     3421 gcgatagccg ccacaacccc agcaccagaa gcccatagac gaccgccgat agaacaacgc
     3481 gcagactgac gtagttgacc aggtagcggc cgctctcctc gcgtcgcgcc ggcacctcgc
     3541 gcaccatgat ctcgtgcagc cccagcgccg acaggccaaa gacgaaggtg aagtaggtcg
     3601 tggccaggct gaagctgccc gacgccgccg ggccgagctg gcggccgatg aggatgaaca
     3661 gcagggcgtt gcctgccttg acgaggacgc tgctcgcaaa cgccaggaag gtgttgctaa
     3721 gcaggcgctg gagcgtgttc atcggcgggg ggcttcacgg cgatgcaggc ccagcccaac
     3781 gtgtggtcct tcacccggtc gagccggtcg agatagaaca gcagcagcgg cagcagcagg
     3841 aagaagacca ggtagacggg caggatgagc ggcagcttca gcaggtaggc cagatcgttg
     3901 tcgccgcgcg ggaagagccg gtcgtgcatc atctggaagt tgaacgacag gaaccagaag
     3961 tagccgcccc acggccgcat ctcgacgacg cgcaggccgg ccttctcgag cagatgggtg
     4021 aagccgtagg aggtgtagcg gaagaagtcg tggggcttct ggtgctggtg ccaggccatc
     4081 ggcgcggaca ggtagaagcg gccgccgggg cgcaggacgc ggccgatctc gtcgatgacc
     4141 agcgccggct cgttaacgtg ctccaacacc tggaagcaga ccgcggcgtc gaagctgtcg
     4201 tcggcaaagg gcagatgcga caggtcgccc tgggcgtcca acccggtgta gtcccacgtc
     4261 tggtcgccca cggccagatc gacgccgacg tagcgcaggt gcttgaaggt gtcgcggtac
     4321 tgcccttcgc cggagccggc gtctaacaga cgcgcgccgg gtggcagttc cgcctgggcc
     4381 tgagccaata ggcggttggt ctcccataga tgaacgtcca gcttttcgct gacccacctg
     4441 ggcagcaggg ccagttgccc ctctcgtcct agtcgcatat gtcacactcc caaaccgcca
     4501 ttgtagcggc cccgcgcccc accgccaacc agcgtagggc gatttaccaa atcgcccgcg
     4561 atttaccaaa tcgcgctaca acggcgggcg tggtaacatt ggccgcgtga ctgccatgca
     4621 aaccaactcc aacacatcca ccaacaccca cccctggtcg ccgtcgctga aggcccggct
     4681 ctatgggctg gcccgcccgg ccgaggaatg gctgctgcgg gcagtctaca cctcgcgctt
     4741 ctaccagtgg tcgaaggccc gcgccgccgc gccaaccctg gcgacgcgct tcgacctcta
     4801 tcagtacgtc atcgacgccc acggcttggg cggaacaatc gactatctgg agttcggcgt
     4861 ctaccacggc gagtcgctgc gctggtgggc ggcgcacaat agcgcccctg cttcgcgctt
     4921 ctacggcttc gacgtcttca ccggcctgcc cgatgcctgg gtgggcctgc ccgcgggcac
     4981 cttcaccacc cacggccagg tgccaacctt cgacgacccg cgcgtggagt tggagatagg
     5041 cctgttccag gacacgctcg gccccttcgt cgacggccac gcgctcgacc ggcgcacggt
     5101 catccacctc gacgccgaca tctacacctc gacgctttac gtcctgacga cgctggcccc
     5161 ccggctgcgc cccggtgatg tgctcctgtt cgacgagttg ggttcggcct acggggtcag
     5221 tcacgagttc cgcgcgctgg aggacttcgc ctcatcctac cgctgccaat accggctgct
     5281 ggcagggacg cgacgctatt tgcaggccgc catcgagctg ctctagcgcg gcgctagggc
     5341 gcggacaccc cgcgcgccca ggccggcggc ggcgtggttg cccgccccgt gcgccagccc
     5401 agccacagcc cgcgccagta ggcccgcagg gccgtggttt tgcccgccag cagcagccgc
     5461 gccgtcatct tgaccgcgct ggccagccgg aaggggacga tgagcagcag ccgcaggcca
     5521 cggccatgct tgcgaaagta gcgcccgctg ctctgggcca tccagtagcg ctccaggggg
     5581 ctgtcgcggc cgccgctgct ggcgctgacg gcatggagga tgtgggccga ggggcacagg
     5641 cggatgcgcc agccggccag ccgcgcccgc cggcagtagt ccatatcctc atagtagagg
     5701 aagaacgcct catccagcag cccgacggtc gcaaacacct cgcgccggat gagaatgcca
     5761 cagaaggggg cgaaatccac gtcgcgcggc gtggcccact ggccgacgtc gcgctgcccc
     5821 tggccgccgt ccttcaggtc gaggaagacg ttcaggttcg cgcccactgt ccagatgcgc
     5881 tccggctcgg tggcgtaata aatcttcgcc gtgaccaacc cgatgaactc cccgcgaccg
     5941 tctccgcctg gcgccctagc gtcctggcgt cctggcgtta gcctctcctc ttccatctgg
     6001 gcaatcagtt ggtcgagggc gtccggggcc agcagggtgt cgttgttgag cagcagcacg
     6061 gccgccggct cgccggccaa cacgtggcgc aacccggcat tgtagccgcc ggcaaaaccc
     6121 agattgcgcg gcagccgcag cccttccacg gccgggaagc gctccgccac ggccgcggcc
     6181 agcggcggcg cgctgccatt gtccaccagc acgacgcggc cgggcgcgtg gcgcagcgcg
     6241 gcgacactgg ccaggcagtc gagcgtcttg tcgagctggt tccaggcgac gatgacgagc
     6301 gcgatggcgt cggcgttcat ggccgggtgg cgaagccgct gtcgatggct cgcgccgcgc
     6361 cgcagcaata gagcaccttg ctggcgtaga cgacgggcag gctgggccag tagcgccaga
     6421 gggcgcgctt ggcatagacc ccggcggtga tgcgcgtggc gatgagcggc gacagcagcc
     6481 gccaccaggc cgcgcggcgc gccccggccg gcgtgccgaa ctcctccggg tagcgcagcc
     6541 gcacgcggac ggcgttatgg cccaggttgc gccagtggcg ggccacgtcg gcgcgggtgg
     6601 tgcgcgccgg gctgtgggcg acgacagccg ccgggtcgaa ccacaggcgg tagcccgcgc
     6661 ggcgaaggcg gatggtccag tcggcatctt cggccgccgc gccggggaag gactcgtcca
     6721 tgccccccac ggcggcgaaa acctcgcgcc gcaggctgag gttcagcgtc ggcagcagga
     6781 cgcgcgcccc ggccggtcgc tggggaatga actcgtggaa catggacacg ttgtcgctct
     6841 gcgcccagta gttgctgccg gccagggcca ccgcgccgcc gacgacctgt tccccggcga
     6901 cgtggcgggc gcggtgggcg gccagccagc cggggtgggg aatgcagtcg gcgtcgagca
     6961 gcagcagcag ttcgcccgtc gcggcggcca ggcccaggtt gcgcgcccgc gcggcgcaga
     7021 ccggcgcgcc ggtgtcgatg aagcgcacaa tccctccccc taaccccctc ccagagggcg
     7081 gggggacgcg cgtcgggcca ctctcagagg gtagggggac gtgcgtcggg ccactctccg
     7141 agggcggggg gaaagctttt agagcgccgg cctcgtcgcg gccgacgacg atgatctcgt
     7201 ccggcaattc cgtgcccgcg gcaatggcct gtaacgcccg gctcagggtc ggcgcgttca
     7261 ggctggggat gatgaccgag gcgcgcacgt caccacaccc ggccgagcag ccccttcaga
     7321 taggccgact caggaaatga gagcagcacc ggatggtcgg cggcctgccc cagatgttgc
     7381 agaatctgca cgtctcgccc ggcatcgacg gccgcgccaa agacgacctt ctggaagagg
     7441 tcggccgaga ccaggccgga gcaggagaac gtcgccagca gcccgcccgg ccgcagcagc
     7501 cgcagcgcca gccagttgag gtccttatag ccgcgacagg cgcgctcgac ctgcccctgg
     7561 ctgtgggcga acttgggcgg gtcgaggacg acgacgtcga actgccggcc cgtctcgcga
     7621 tagtagcgca gcacctcgaa ggcgtcgccg gcgatcaact cgtcgtcggg ccggtcgaag
     7681 ccgttgaggg ccatgttgcg ctcggccacc tccagcgccg gcgcggagct atcgacctgg
     7741 gtgatgcgcg ccgccccggc ggcggcggca tagacgccga acgcgccggt ataggcgaag
     7801 acgttgagca cctcgcgccc ggccagcagc gccggccggc cgacggcggc gcggttgtcg
     7861 cgctggtcca ggtagaagcc cgtcttgtgc ccgcgccaga catcgaccag gaaggcatgg
     7921 ccgttctcgt ggatggtcat ctccggcggc gggggctggc cgtggcgcgg ctcgatcagc
     7981 agccgcagcc cctccttgcc gcgcacgtcc acgtccgacc gctccagaat gcccgccggc
     8041 cgcagcagct ccgccagttg cgccgtcagc atcgccttgc gccggtcgat gcccagcgtc
     8101 aggcactgca taaccagatg gtcgccatag cgatcgacga taaggccggg caggccatcg
     8161 gcctcggcgt taacgaggcg gtaggcggtg gtgggggagg caggaaccgg ggagccgggg
     8221 agccggggag ccggggagac aagagcggcg ccggcgtcgc tgctgaggcc cagagcggcg
     8281 cgggcagcga tggcgcgttc caggcggcgg ccccaaaagg catcgtcgat cggctcggcc
     8341 gggtcccagg aaagaatgcg ggcgacgatc tgcgacctgg ggttgaaggt tgccgtggcc
     8401 agagggttgt cgcgctcgtc ggtgatggtg atcaggtcac ccggctgcgg gtcgccatgc
     8461 aggcggccga ccgcgccgga gaagacccac ggatggcgat tgtgaaccgg cttgtcgcgg
     8521 cccggcttga gttggaggct ggcggttggc gtttccatgg gggagtcagt ggtcagtggg
     8581 tcagtggtca gtggtcagtg gtcagtggtc tgtcgtcggc ggtcggcggt cggcggtcgg
     8641 cggtcggcgg tcggcggtcg gccgtcagtg ctccgcgggc aaggtcgccg ccgccgcccg
     8701 cgcccgcgcc ggccagagtt gcgagacgag gatgccggcc agcatcaggg cgcagccgag
     8761 gagggcgcgg gcggtcagtt gctcgctcag cagcagccag ccgcccagca cggcaaagac
     8821 agactccatg ctcatcagga tagccgcgtc ggccggcggg gcgtggcgct ggccggcgac
     8881 ttgcagcgta tagccaacgc caacggacaa gacgccggta tagaggatgg gcagcgccgc
     8941 ggcgcgcacg tcggcgggcg tcgttgtctc cagcagcagc gccgccagcc cgctaagcgc
     9001 ggcgcagacc atgaactggg tgaaggccag cttgacaggg tcgcggccgg gcgagaaatg
     9061 gcccagcaaa agcatatgca cggcccacgc gaacgcgccg aggagcacca ggaagtcgcc
     9121 caggcccatg cggaacgacc cttgcatggt cagaaagtag aggccggccg cggccagcag
     9181 cacccccacc cacgtggccg ccggcgcgcg ccgccccagc gccagcccca gcaggggcac
     9241 gatcaccacg tagaggctgg tgatgaagcc gctcttgccg gccgtcgtgc gggccacgcc
     9301 gagttgttgc agcgtcgccc cggcgcagag ggcgacgcca acgaggatgc tggccaggtg
     9361 atgcttgctc agtaacgtcc ggcggccagc gccggccgcc gccggccggc cctggcgctc
     9421 gcccgccagg atgatcggca gcagcaccag cccgccaatg aaaaagcgga tggctgtgaa
     9481 cgccatcggg ccaatatgct ccacgccggc gcgctgggcc acgaaggcga agccccaaat
     9541 gaaagcggcc aacagcagca gcaggtcgga gcgcaggcgc aacgggtcgg aatgcatggg
     9601 ggccattgta gccgctccgg cgcggcgcgg ttagggctgc ggccggaagc ggctgatgag
     9661 cgcctcgttg actgccgccg cggcctcgtg ctggagccag tgggtgttgt cggggaaggc
     9721 cagcagctcg ccgtcgtcgc acagcgcggc gctctcctcg gccatgaccg cgcgcagggc
     9781 gaagtccttc atgccccata agatcgttgt cggcacgacc acgcgtgtcg aggacacctc
     9841 ggccggcggc cgccgcacca gggcgcgata ccagttgagc atcgtcgtca tcgcccgcgg
     9901 acgcgaccag gcccggcggt actcctccag gtcagcgtcg ctgaacgtgc ccggcaagct
     9961 cgtgtcccgc aacgtgcgcg ccagcagccg ccaatcgttg gcctccatta gccgctccgg
    10021 cagccccggc agctggaaaa aggccgcgta gaggctgcgc gacatctgcg ccgggtcggt
    10081 ggtgaggtgg ttgagcatga ccgcggggtg ggggacgttg aggatggcca gccgctgcac
    10141 ccggtgtgga gcgatcagcg ccacccacca ggcgaccatc gccccccagt cgtggccggc
    10201 caggtagacg cgctcccggc cgctggcgtc gatcagcccc agggcgtcgt tggccagcgc
    10261 gtcgagcgag taggccgaca gcgcgcgcgg ccggtcgctg aggttgtagc cgcgctggtc
    10321 gggagccagg acgcgaaagc cggccgcggc cagcgccgga atctgctgcc gccagccgta
    10381 ccagaactcc ggaaagccat gcagcagcaa gacaaccggg ccatcctccg gcccgtcgag
    10441 cacggtgtgg agggtaacgc cgttggtggg gatgaggcgg tggagcatgg ggtagtggtc
    10501 agtggtcagg gtcagtggtc ggcgctcaaa cctcgcggtg gccctgcccg ctctccggtc
    10561 gcgcggtcag caggtcgagg gcgtggggca agacgggcag cagcacgtcg aggttctcgc
    10621 gcacggcctt ggggttgcca ggcaggttga tgatcagcgt ctggccgcgc agcccggcca
    10681 cgccgcgcga cagcatggcg tggcgggtga cgtgcaggct ctcggctcgc agcgcctcgg
    10741 caatgcccgg cgcatcgcgc tcgatgacca cccgcgtggc ctccggggtc acgtcgcgcg
    10801 gggcgaagcc ggtgccgccg ctggtcagca gcagctccag cccgtcgtcg caccactggc
    10861 gcagcgcccg gacgatggcg tcgaagtcgt cgggaatgat cgcctgccgc tcgataggcc
    10921 aaccggtggc cgcggtgacg gccgcggccg cggccggccc actctcgtcc gcgtagtcgc
    10981 cggccgcggc gcggtcgctg atggtcagga tgccgactcg cctcattcgc gccgcggctc
    11041 ctccaccagg gccagccatt cctcgctcag gccgctgtcg gtaacgccgt agtagacgtg
    11101 gacgccatcg tcggcgcgca cggccaggtc gtagagcgtc gagttgccct tcggccgccg
    11161 gttgagcacg gccacgttct gccgccactg gacgctaccc aggttgggcg gggttgtttc
    11221 cgcctgggta atggcataat gccatagccg ccgcgccgag gagcgggtga cgttgttgat
    11281 caggttgccg ctgcgcaggt cgcgcagggt gtggtagatc gtgccgtcgc gctcctggct
    11341 ctcgacgagt tcgacgcccg tgcgcggccg ttcccgcttg ccgttggtcg atggcgcgct
    11401 ggccggcggc ggcttctccg cgggcgtggc ccgggccgtt tcggagcggc ggcggtcggc
    11461 acgcggggcg ggggcggcgg ccacgggcgt gggcgtgggg cgcgccggct cctcggccgg
    11521 cgcggcgatc tggcccgccc ggcgctccag cccgcgctcg accaattgga cgatctcgtc
    11581 gcgcacggcc agcgtcgtct ccgtctcgtc gcggacgtag aagaggttgg agtcgatggc
    11641 gtagggcgcg tcggggccgg gcgtcacggt gacgcgcacg acggtcgtgc cctgcgttgg
    11701 caggctgtcg atctgcaccg gcggcttcgg gctgaagcgc ttggccagcg tcgtccgcag
    11761 cgcctcgatg gccttggccg gctcgcggac accctccgcc ttggcctttg ggtcagttgg
    11821 cacgccaacg tagatcgtgc cgccgttggt gttggccatg gcgcagatgt cgcgcagaat
    11881 gttgtccagg tggccgccac gctggttgat ggtcgggtgg aaggcctgca ccagcgactc
    11941 gccttcctcg cgggccagtt ggacgaaatc gaccgccttg gccgtgccgc ggtaggggcg
    12001 ggtcagggag tagtcgttgc cgcggatgac gccggccagg gccgcgaagg acacttcctc
    12061 cagcaagatc tccgtcacgc ggtcgccgac gcccagatag ggcgacgtgg ctgattcacg
    12121 gatgagccgg tgggcgtcgg aaccctggat gcagcgcatc ggccgcgggt actctggctt
    12181 gctgccgtcg aagaagcggc gcgtcgtctg gttgccgcgc ttctccaggt cggtcacttc
    12241 cagcgcgtgg acgttggggt cctgcgtata ggcgacgcgc gtttgcccgc cgaaatcgag
    12301 gccgcgcatc atcacgccgt tgccggaatt cacatgggcg gcgatgacca gcccgccggc
    12361 ctcgttgatg acgtgatagg cggtcagtac gtcggaggtc gcgcccacgg tcgaactgcc
    12421 ctcgcgcagc agcgccggcg gcacgcgcag cgtcagcagc aaatgctcca gatagctcac
    12481 cggcgtctcc ggcgggaaga tgcccagaat atgaaaaccg aaggtggcgg tgaactcgaa
    12541 gccgggcagg acaagcagct tgtccagc
//
LOCUS       DAOSMQ010000018        12385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5097_length_12385_cov_4.634955, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000018 DAOSMQ010000000
VERSION     DAOSMQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12385
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5097_length_12385_cov_4.634955"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /gene="menD"
                     /locus_tag="PK829_01240"
     CDS             complement(<1..1620)
                     /gene="menD"
                     /locus_tag="PK829_01240"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOU39866.1"
                     /translation="MDLSSNANTRWAASFVEELANCGLEAVCLAPGSRSTPLALAFAA
                     HPDIRLYPHLDERSAGFFALGLALATDKPVALVCTSGTATANFHPAIIEAYYSYVPLL
                     VLTADRPPELRGSGANQTIDQIKMFGDHVRWAVDLPTPQDDAPDVVLRHLRTLAARAY
                     ATASGLVRGPVHLNFPFRKPLEPSSTSQLAAAQPPPALARPYTLFGRGRLLPDDTAIG
                     LIVEAIEWHPRGLIICGPCCPGGAFPAVLAALAETTGFPILADPLSNVRHGPQVIDGL
                     VLGAYNLWLHAPAERLPRPEVILRFGAVPTSATLATYLAGLKPNFHVHIREDGQWADD
                     QHQTDTYLQADAAAVCEALAAELARRGFRRQPDWAAPWLAAETRAWAALDERRSAAGF
                     FDGAAIARTLVALPDGAVVFAGNSMPVRYVDAFDRPDARPIVLYGNRGASGIDGNVST
                     ALGLAAAEGRPVVALLGDITFYHDMNGLLAVGQHALNNVAFVITNNDGGGIFRRLPIA
                     HHDPPFTDLFLTPHGLTFDHAAAMYGLRYRQV"
     gene            complement(1621..3111)
                     /locus_tag="PK829_01245"
     CDS             complement(1621..3111)
                     /locus_tag="PK829_01245"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HOU39867.1"
                     /translation="MITHLPARPPTASPPFSATDPAWDNSTPPPGEPRQLISLYAPAP
                     AGLAAAFVRAAGGRERFYWAEPAHGLVLAGAGVAAQLISPPVLADAERLPEQRFSDIE
                     AQARRLFTGALLRPASGADLAPGLAVLARPRLCGGFAFQDDFVPDNTWSVFNPAEFLL
                     PHYQYAQKGDAACLTINALLGPGEDLDEALRGLAEALRLYLAATLTLAVAPAAAPIGP
                     TTLRYPMTAPQWADIIGRAVAAIRAGALDKVVLARVGEVWAEAAIDAAAPLAVLDADY
                     GDSYRFLFEPCPGHAFLGATPELLAGKSGRAVATMALAGSVARGRSPAEDDALAAQLL
                     TSAKDRHEHQFVVDAIRAQLAEAADALDVPATPVVLRLRNIQHLHTPIRGQLRRPDDS
                     ILRLVRRLHPTPAMGGVPRDRALAFLRDAEPFPRGWYAAPIGWFDSAFDGVFAVGIRS
                     AVTQHARVWLYAGAGIVAQSTAEREWAETALKFRPMLGALGVGEMG"
     gene            complement(3108..3758)
                     /locus_tag="PK829_01250"
     CDS             complement(3108..3758)
                     /locus_tag="PK829_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU39868.1"
                     /translation="MTTPDADKPTAWRDPNATRERILDAALLTFSRQGYHGTRLDDIV
                     GASETSKGAIYFHFANKERLFLALVDQFADLLERRVLEAIETQGRGMARVRVALETVL
                     ETFGRYRRPAKILLVQAVGLGNVFENKRAAVTDRFARLIEGYLREAIELGDIEPTDTE
                     VVAYAWMGAIYGLVIRWVTTGEPEPGRIMTGLVPLLLRGVGYEDRRAAMTTGDPTA"
     gene            4116..4889
                     /locus_tag="PK829_01255"
     CDS             4116..4889
                     /locus_tag="PK829_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOU39869.1"
                     /translation="MNRSVVGAAETNSGAASGGDRERASSGRRRVSPSAWLYGLASLL
                     ALSIVAFNVIRPIVVLPRIAPSPGFALVDSTGQPVTSEDRRGFLTLYSFSYLDCDVQC
                     PQSVDDIAALRAAVAATLPAGTPLSFVTISLDPENDTPARLATAASAHALPADAPGWL
                     LLTGDPARTRATVGGGFGVYYSPPDLQAGDASPRVRFDPRYVLVDHLGIMRAEYRTGT
                     PDPALIARDVNLLLKEAANSNGVVRLGYEAAHLFLCYPR"
     gene            4898..5566
                     /locus_tag="PK829_01260"
     CDS             4898..5566
                     /locus_tag="PK829_01260"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOU39870.1"
                     /translation="MALQPDKTDATMKPPRAKRPSALLWAGLVALLLIGGVAVWSLVN
                     GRLPFVPPQLHGTVVQSPDRVPDFTLDASTGERVSLSDLRGKVLLVYFGYTFCPDACP
                     TTLNELKKVPAVLGQSADDVQVVMISVDPQRDTPEVLREYLAHFDPSFLGLTGTDEEI
                     LAAATPLGIYYNAHEGSAATGYLVDHTTSVLAIDREGYLRVIYSFETPGEAIAEDMRW
                     LVRQ"
     gene            5631..5759
                     /locus_tag="PK829_01265"
     CDS             5631..5759
                     /locus_tag="PK829_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 2A"
                     /protein_id="HOU39871.1"
                     /translation="MNDKDRYKTPQGTIALMVIFIILIIALWGSAYLTLLSRGVTQ"
     gene            5759..6241
                     /locus_tag="PK829_01270"
     CDS             5759..6241
                     /locus_tag="PK829_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOU39872.1"
                     /translation="MHIDRYERLFLSAAVALLVVFMGALAVSSFAYGIRVPTATERVD
                     PRTVATPPSPWGEPVENRLRELSPGNYEVYILAQMWQFTPATITIPQGSRVTFYVTSK
                     DVQHGFKLSGTNINMMALPGEVARLTATFDTPGTYNFICHEYCGALHHTMYGQLVVEE
                     "
     gene            6270..7979
                     /locus_tag="PK829_01275"
     CDS             6270..7979
                     /locus_tag="PK829_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="b(o/a)3-type cytochrome-c oxidase subunit 1"
                     /protein_id="HOU39873.1"
                     /translation="MTKRKAQAGDVAGVAALPPGTFKREDRLTAWYITIAMVALFIGG
                     SFGPLQKLEHVGINLYPTLAKIGIGSVRIQSYYQGLTAHGVLNALVWTTFFIVGFFTY
                     AVPRSLGRPLSRPRVNLLALVMMITGLIMAAIPILTNLATVLYTFYPPMKASIFFYLG
                     LVLVVVGSWVGGLSYSTYWQWRKDNPGQKTPFIALASIITMVMWQIATLGVATEILAL
                     IIPWVLGWTEGTDPQLARTFFWFTGHPLVYFWLLPAYVSWYAMLPKQAGGKMFSEQLA
                     RLVFWLFLVLSIPVGLHHQFLDPGVPAGWKAIHAVITYSLFLPSMATAFTVVASLEIG
                     GRARGGKGLLGWIPKLPWGNPSYTAQNLAMILFVFGGIGGLTNASYNLNLAIHNTAWV
                     PGHFHLTVGSAVTLTFFGIAYWLVPALTGKPLASRRAALAQAWTWFIGMLLMSNALHI
                     LGLNFGAPRRTALGLSPYVDPAWNPLQIEAAIGGIILGISGLLFFGNMLATALSRQRA
                     EVRAEMPVAEPLHPEYETPGWLDSWRPWIVATVLLILVAYGPVLVSMIANINLTSPGV
                     QIR"
     gene            8030..8467
                     /locus_tag="PK829_01280"
     CDS             8030..8467
                     /locus_tag="PK829_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOU39874.1"
                     /translation="MSKKTLLMLLISLFLLALAACGGGGNTDETTADNTGDTAAVGNA
                     ANGEKLFSQATIGANNAPGCITCHSLEPDVVIVGPSQAGLATRAESRVPGMTAAEYIH
                     QSIVEPNAYVVEGFAEGLMYQNYATDLTEQEINDIVAYTLTLK"
     gene            8618..10879
                     /locus_tag="PK829_01285"
     CDS             8618..10879
                     /locus_tag="PK829_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOU39875.1"
                     /translation="MSAPPPTDPVPTRASDQAPRDHITWLTGPLPAAGTGDKVAAWPP
                     RRGRWAIALERVALAAERVVNYVTTAKYNPLYYTGTIAFFLLLIVGLTGLYLFLFFQY
                     GYDASYAAVGRMESQFIARTIRAIHRYASGATVIVTLLHAYRMLFMERFRGPRWLAWL
                     TGVALTGIVWFAGITGYWLIWDERAVLINDTFGRALAALTPWQPAFMVYLSGAEAAGR
                     SWILFLLVLAVHVILFLVAVGFFYLHIVRLKRPKWLPPLPWVAGIGVIMLLSGLLFPI
                     GMLPQGDLLRFPQAITFDPIFLFFLALPGGPWLWVALLAATLVTAALPWLSRRQRAAA
                     GRAATFPPAHPDAPRVRVLGERCTGCTLCALDCPYGALEMVERDDGRPHKYIAREIAD
                     RCVSCAICVGSCDVLAITLGEAMPEDLWTLMAAGGPAGGGPAAGGPAATWSGGDAGDP
                     IGLNARRLAGARLVFTCERHAAHGARAYLTGDRAADAPLVVIPLPCIGAAPPDLLSRA
                     LEAGAASVSVVGCPPEDCANREGNAWAEQRLTRQRLPRLRKPYIDAPVTAAYLPPDAF
                     AQGLRALAPATGGGAPDYAASRGMGRTFSLRHFGPALALLAVALLLQILLTDVPLRPD
                     ANRPAVVQLVTGDLGATFGSLVAGQTADSAYRLEVLVDGREVLRRSFTAEEVVGLDRD
                     ARAQFFHELPVAPGAHTLTVRLIGTTTGATFVLFDDHVTVSAGQATQPALWPQSLWLD
                     GPRPCVSDHACAQ"
     gene            complement(10873..11646)
                     /locus_tag="PK829_01290"
     CDS             complement(10873..11646)
                     /locus_tag="PK829_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU39876.1"
                     /translation="MPLLRLPTHDRLAYIDTGPTGGSPLLLIHGFTGTATADLGNLIA
                     EFAPAHRLLAPDLRGYGQSRPPNRDFPPDFYERDAVDMAALLDAVQPGPVVVLGFSDG
                     AEVALLLAAARPDLVRGVVAWGVSGVISPEELAGVAKWLPATAWGPEREQWKQQIITR
                     HGAEQFPAMVEGWVAAAQAIAAAGGNICYQQAANIACPVLLLNGDGEEGNTPRDVRRL
                     AERIPGARLEFVSDSGHAIHRDRPDVLLARIRAFLAEGY"
     gene            complement(11663..>12385)
                     /locus_tag="PK829_01295"
     CDS             complement(11663..>12385)
                     /locus_tag="PK829_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008941402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOU39877.1"
                     /translation="IYDVREIIARLVDGSRLTEFKARYGPTVVCGFAHILGIPVGIVA
                     NNGLLFSESSLKATHFIQLCGQRGTPLLFMQNIAGFMVGRQYENEGIAKHGAKMVMAV
                     SNVNVPRITLLHGVSHGAGNYGMSGRAYDPRFLFSWPNSRISVMSGDAAADTLWTVGK
                     RQVFHQMDEPTDSEIAAAEEKFKRPILEQYARESSPYYATARLWDDGILDPVKTRAAL
                     ALAYAATAHAPLPPGGYGTFRM"
BASE COUNT         2021 a         3987 c         4157 g         2220 t
ORIGIN      
        1 cacctggcga tagcgcaggc cgtacatggc cgcggcgtgg tcgaaggtca gcccgtgggg
       61 cgtcaggaac aggtcggtga agggcgggtc gtggtgggca atgggcagcc ggcggaagat
      121 accgccgccg tcgttgttgg tgatgacgaa agcgacgttg ttgagggcgt gttgccccac
      181 ggccagcagc ccgttcatgt cgtggtaaaa ggtgatgtcg cccagcaggg caacgaccgg
      241 tcgcccctcg gccgcggcca ggcccagagc cgtggacacg ttgccgtcaa tgccgctggc
      301 cccacggttg ccgtagagga cgatggggcg cgcgtcgggc cggtcgaagg catcgacgta
      361 gcgcacgggc atgctgttgc cggcgaagac gacggcccca tcgggcaggg ccaccagcgt
      421 ccgggcgata gccgcgccat cgaagaagcc cgccgcggat cgcctttcgt ccagcgccgc
      481 ccaggcgcgg gtctcagcgg ccaaccacgg tgcggcccaa tcaggttgcc gccggaagcc
      541 gcgccgcgcc aattcggccg cgagcgcctc acacacggcc gccgcatcgg cctgcaagta
      601 agtgtccgtt tggtgctggt cgtcggccca ttggccgtct tcgcgtatgt gcacgtggaa
      661 gttgggtttc agcccggcca gataggtcgc cagggttgcc gaggtgggca cggcgccgaa
      721 gcgcaaaatc acttcgggcc gtggcagtcg ctcggccggc gcatgcagcc acaggttata
      781 ggctcccaac accagcccgt cgatcacctg cgggccgtgg cgcacgttgg acaggggatc
      841 ggccaggatg gggaagcccg tcgtctcggc cagggcagcc aatacagccg ggaacgcgcc
      901 gccgggacag cacggcccgc aaatgatcaa tccgcgcgga tgccactcga tggcttcgac
      961 gatgaggccg atcgccgtgt catcgggcaa caggcggccg cggccaaaga gggtgtaggg
     1021 ccgcgccagg gctgggggcg gttgggccgc ggccagctgg ctggttgacg acggctccag
     1081 cggcttgcgg aaggggaagt tgaggtgaac cgggccacgc accagaccgc tggcggtggc
     1141 ataggcgcgg gcggccagcg tgcgcaggtg gcgcagcacc acgtcgggcg cgtcgtcctg
     1201 aggcgtgggc agatcgaccg cccagcgcac atggtcgccg aacatcttaa tctgatcgat
     1261 ggtctggttg gccccactac cacgcaactc gggtgggcgg tcggcggtca gcaccagcag
     1321 cggcacgtag gagtagtagg cttcgatgat ggccgggtgg aagttggccg tggctgtgcc
     1381 cgacgtgcag acgagggcca ctggcttatc agttgccagc gccaggccca gggcgaagaa
     1441 gccggcgctg cgctcgtcca gatggggata gaggcgaatg tccggatggg cggcaaaggc
     1501 cagcgctagc ggcgtggagc gcgagccggg ggccaggcaa acagcttcta ggccgcagtt
     1561 agccaattct tcaacgaagc tggcagccca gcgggtgttg gcgttgctgc tcagatccat
     1621 ctaccccatc tcccccacgc ccagcgcgcc gagcatgggc cggaacttca gcgccgtctc
     1681 ggcccactcg cgttcggccg tcgactgggc gacgatgccc gcgccggcat agagccagac
     1741 gcgcgcgtgt tgggtgacgg ctgaacgaat gccaacggca aagacgccat caaacgcgct
     1801 gtcgaaccag ccgatgggcg cggcgtacca gccccgcggg aagggttcgg cgtcgcgtag
     1861 aaaggccagc gctctgtcgc ggggtacgcc gcccatggcc ggggttgggt gcaggcggcg
     1921 cacaaggcgc aagatgctgt cgtccggccg ccgcagttgg ccgcggatgg gcgtgtgcag
     1981 gtgctggatg ttgcgcaatc gcaagacgac cggagtagcg ggcacgtcca gtgcgtcggc
     2041 cgcttccgcc aactgggcgc gaatagcatc gacgacgaat tggtgctcgt gccggtcttt
     2101 ggccgaggtc agcagttgcg cggccagggc gtcgtcctct gccggggagc ggccacgggc
     2161 tacggagccg gccagggcca tcgtggcgac ggcgcgaccc gacttgcccg ccagcagttc
     2221 cggcgtcgct cccagaaagg catgacccgg acatggctcg aataggaagc ggtagctgtc
     2281 gccgtagtcg gcgtcgagca cagccagggg cgcagccgcg tctatggcgg cctcggccca
     2341 gacctcgccc acgcgagcga gcaccacctt gtccagcgcc ccggcgcgaa tggcggcaac
     2401 ggcgcggccg atgatgtccg cccactgggg ggcggtcatg ggatagcgca gcgtcgtcgg
     2461 gccaattggc gcggcggccg gggccacggc cagcgttagc gtcgccgcca gatagagccg
     2521 cagcgcctcg gccagaccgc gcagggcctc gtcgaggtct tcgcccggcc caagcagggc
     2581 gttgatggtc aggcaagcgg cgtcgccctt ctgggcgtac tggtagtggg gcagcaggaa
     2641 ctcggccggg ttgaagaccg accaggtatt gtcgggcacg aagtcatcct ggaaggcgaa
     2701 accgccgcag agccgggggc gcgccaacac cgccagcccc ggggccaggt ctgccccgct
     2761 ggctggccgc agcagcgcgc cggtgaataa ccgccgcgcc tgggcctcga tgtcgctgaa
     2821 acgctgttcc ggtagtcttt cggcgtcggc caacaccggc ggcgagatga gttgggcggc
     2881 cacgccagcc ccggccagca cgagcccgtg ggccggctca gcccaataga accgttcccg
     2941 gccgccagcc gcgcggacaa aagcggcggc caggccagcc ggcgcgggcg cgtagaggct
     3001 gatcagttgg cgcggctcgc caggcggggg cgtgctgttg tcccaggccg ggtcggtggc
     3061 cgagaacggc ggggatgctg tgggaggacg ggcaggtaga tgggtgatca tgctgtgggg
     3121 tcaccggtcg tcatggccgc gcggcggtct tcatagccca cgccacgcag cagcagcggc
     3181 accagcccgg tcatgatgcg ccccggctcc ggctcgccgg tggttaccca gcggataacc
     3241 aggccataga tcgcccccat ccaggcgtag gcgacgactt cagtgtcggt cggctcaatg
     3301 tcgcccagct cgatggcctc gcgcaggtag ccttcgatca ggcgggcgaa gcgatcggtg
     3361 acggcggcgc gcttattctc gaagacattg cccagtccaa cggcctgcac caggagaatc
     3421 ttggccgggc ggcgatagcg gccaaaggtt tccagcaccg tctccagggc gacgcgcacg
     3481 cgggccatgc cccggccttg cgtttcaatg gcctccagca cccgccgctc caacaggtcg
     3541 gcgaactgat cgacaagcgc caggaagaga cgctccttgt tggcgaagtg aaagtagatc
     3601 gcccctttgg aagtctccga ggcaccgacg atgtcatcca ggcgcgtgcc gtggtagccc
     3661 tggcgcgaga aggtcagcag ggctgcgtcg aggatgcgtt cgcgtgtggc gttggggtcg
     3721 cgccaagcgg tgggtttgtc cgcgtcgggt gtagtcatag gccgcagttt actgtaaacc
     3781 gactggtcag tctattgttt cagccaaaaa agaacgaata tacagtcacc catgagcagc
     3841 gcattgctgt tcacaacatt caaaacattc tgaatctaat cgtgaagtct atcacaaatg
     3901 gtggcaaaat caatagggaa gccaaaaaac tgtttcgcaa acctacaaaa cttaaacgta
     3961 tctgagtata atcggtcggg ttcattcatc gacagggttg gcgcggtcac gatatacttg
     4021 accgagtaag tagcattggc gtcgttgggg gcggggagac gctcaccatg gcgacactga
     4081 gaactgagta tgttttgtgg atggagtaga gcctgatgaa tcggtcagtc gttggcgccg
     4141 ctgagacgaa cagtggggcc gcgagcggtg gggatcggga gcgcgcttcc agcggccggc
     4201 gtcgcgtgtc gcccagcgcc tggctttacg gcctggccag cctgctggcg ctcagcattg
     4261 tggccttcaa tgtcatccgg cccattgtcg tcttgccgcg catcgcgccg tcgccgggct
     4321 ttgctcttgt agatagcacc ggtcagccgg tcaccagcga ggatcggcgg ggattcctga
     4381 cgctctatag tttttcctac ctggattgtg acgtgcagtg cccgcaatcg gtggacgata
     4441 tcgccgcgct gcgcgcggcc gtcgcggcga cgctgccggc cggcacgcca ctctccttcg
     4501 tcaccatcag ccttgaccct gagaacgaca cgccggcgcg cctggccaca gcggcgtcag
     4561 cccacgcact gccagcggac gcgcccggct ggctgctgct aaccggcgac ccggcgcgta
     4621 cgcgagcgac cgtggggggc ggcttcggcg tctactactc accgccggat ttgcaggccg
     4681 gggacgcctc gccgcgcgtg aggttcgacc cccgctacgt gctggtagac cacctgggca
     4741 ttatgcgcgc tgagtatcgc accggcacgc ccgacccggc gctcattgct cgcgacgtga
     4801 acctgttgct caaggaggcc gccaacagca atggcgtcgt tcgtctgggc tacgaggcgg
     4861 cgcatttgtt cctgtgttac ccgcgctaaa atagaccatg gcccttcaac ccgacaagac
     4921 cgacgccacc atgaagcccc cgcgcgccaa gcgccccagc gccctcctat gggccggtct
     4981 ggtggcgctg ttgctcatcg gcggcgtggc cgtctggagc ctggtcaacg gccgcctgcc
     5041 ttttgttcca ccccagttgc acggcacggt cgtccagtcg cccgaccgtg tgcccgactt
     5101 caccctggac gccagcacgg gcgagcgtgt tagcctgagc gatttgcgcg gcaaggtatt
     5161 gctggtctac ttcggctaca ccttttgccc cgatgcctgc ccgacgacgc tgaacgagct
     5221 gaagaaggtt ccggcagtgt tgggacagtc ggccgatgac gtacaggtcg tcatgatcag
     5281 cgttgatccg cagcgcgaca cgccagaggt gttgcgcgaa tacctggccc acttcgatcc
     5341 atcctttctg gggctgacgg gcacagacga agagattctg gccgcggcga cgcccctggg
     5401 catctactac aatgcccacg agggttcagc ggccaccggt tacctggtcg atcacaccac
     5461 gtcggtcctg gcgattgacc gcgagggcta tctgcgggtg atctacagct ttgagacgcc
     5521 cggcgaggcg atcgccgagg atatgcgctg gctggtgcgc cagtaaaagt gagccatcgc
     5581 cgccgtagag gtgtcggcgg cgtaaatgaa gtgacaacga aggaggttgc atgaacgaca
     5641 aggatcgcta caagacaccc caagggacca tcgccctgat ggtcatcttc atcatactca
     5701 tcatcgcgtt gtggggcagc gcctatctga ctttgctgtc cagaggagtg acgcagtaat
     5761 gcacatcgat cgctacgaac ggctattcct gtccgcagcg gtggcgctcc ttgtcgtctt
     5821 catgggcgcg ttggccgtca gcagctttgc ctatggcatt cgcgtgccga cggccaccga
     5881 gcgggtcgat ccgcgcacag tggccacgcc gccctcgccc tggggtgagc cagtcgaaaa
     5941 ccgcctgcgg gaactctctc ccggcaacta cgaagtttat attctggcgc agatgtggca
     6001 gttcacgccg gccaccatca ccattcccca gggttcgcgg gtgacattct atgtgaccag
     6061 caaggacgtg cagcacggct ttaagctgtc cgggacgaac atcaacatga tggctctgcc
     6121 gggcgaagtg gcccggttga cggccacctt tgacacgccc ggcacgtata acttcatttg
     6181 ccatgagtac tgcggtgcgt tgcaccatac gatgtatggc caattggtgg tcgaagagta
     6241 aggcgacgcc ttaggaggac acagaatcaa tgaccaaacg caaagcacaa gcaggggatg
     6301 ttgccggggt ggctgccttg ccaccgggga ccttcaagcg cgaggatcgt ctgacggcct
     6361 ggtacatcac gattgccatg gtggccctat tcatcggtgg gtcctttggg ccgctccaga
     6421 agctggaaca cgttggcatc aacctatacc cgacgctggc caagatcggc attggtagcg
     6481 tgcgcattca gtcatactat cagggtctga ccgcccacgg tgtacttaat gccctcgtct
     6541 ggacgacctt tttcatcgtt ggcttcttca cctacgccgt gccgcgcagc ctcggccggc
     6601 cgctcagccg gccgcgcgtc aatctactgg ccctggtgat gatgatcacc ggcctgatca
     6661 tggctgccat cccgatcctg acgaacctgg ccacggtact ctatactttc taccccccca
     6721 tgaaggccag catcttcttc tatcttggcc tggtgctggt ggtggttggt tcgtgggtag
     6781 gcgggttaag ttactcgacc tactggcagt ggcgtaagga caaccccggc cagaagacgc
     6841 ccttcatcgc cctggccagc atcatcacga tggtcatgtg gcagatcgcc actctgggtg
     6901 tggcgaccga aatcctggcg ctgatcattc cgtgggtgct cggctggacg gaaggcaccg
     6961 acccccaact ggcgcggact ttcttctggt tcacaggtca tcccctggtg tatttctggc
     7021 tgctaccggc ctatgtttcc tggtatgcca tgttgcccaa gcaggccggc ggcaagatgt
     7081 tcagcgagca gctggcgcgg ctggtcttct ggctattcct ggtactttcc atcccggttg
     7141 gtctccatca ccaattcctg gaccccggcg ttccggccgg ctggaaggcc attcacgccg
     7201 tgattaccta ctcgctcttc ctgcccagca tggccacggc ctttacggtt gttgcctcgc
     7261 tggagattgg cgggcgggcg cgcggtggca aaggtctgct gggctggatt ccgaagctgc
     7321 cgtggggtaa tccatcatac acggcccaga acctggccat gatcttgttc gtcttcggcg
     7381 gtattggcgg tttaaccaat gcgtcgtaca atctgaatct ggccattcac aatacagcct
     7441 gggtacctgg ccatttccat ttgactgtcg gttcggccgt caccctcact ttcttcggca
     7501 tcgcctactg gcttgtaccg gcattaacgg gtaagccgct ggccagccgt cgtgctgcgt
     7561 tggcgcaggc ctggacgtgg tttatcggca tgctgctgat gtcgaacgcg ctgcatattc
     7621 tcggcctgaa cttcggcgca ccgcgccgca ccgccctggg tctgtctccc tatgttgacc
     7681 ctgcctggaa cccattgcag atcgaggcgg ccatcggcgg catcattctg ggcatcagcg
     7741 gcttgctgtt cttcggcaac atgctggcca cggcactgtc gcgccaacgc gctgaggtgc
     7801 gcgcggaaat gccggtggcc gagccgttgc atccggagta tgagacaccc ggctggttgg
     7861 acagttggcg gccatggatt gtcgccaccg tcctgttgat tctcgttgct tacggccctg
     7921 tgctggtaag catgatcgcc aatatcaatc tgacttcgcc cggcgtacag attcgctaga
     7981 cggttagact ggtgttggcc gagtaatacg tgtacgtgga ggtacaccta tgtccaaaaa
     8041 aacgctgttg atgttactga tcagtttgtt cctgttggca ttggccgcgt gtggcggcgg
     8101 tggcaacacc gatgagacga cggccgacaa cacgggcgac accgccgccg ttggcaacgc
     8161 tgccaacggc gagaagctct tctcgcaggc caccatcggc gcgaacaacg cccctggctg
     8221 catcacctgc cactcgttgg aaccggacgt cgtcatcgtt ggcccgtcgc aggccggctt
     8281 ggctactcgc gccgagagcc gcgtgcctgg catgacggcg gccgagtaca tccaccaatc
     8341 cattgtcgag ccgaacgcct acgtcgtcga gggcttcgcc gaaggcttga tgtatcagaa
     8401 ctatgcgacc gacctgaccg aacaggagat caacgacatc gtcgcctaca cgctgacgct
     8461 gaagtaatcc ctttctggcc acgcctggcc ggctcagcgc cgccaggcaa tctcaatccg
     8521 tacagaggtg gctgtcatgg ccgcctctgt accccacttc tcatcagggg agcgccggac
     8581 tctgtgtccg gcaacgagcc atcgcaccat ctgcgccatg tccgcaccgc cgccgaccga
     8641 ccctgtcccg acccgcgcca gcgaccaggc tcctcgcgac catattacct ggctaacggg
     8701 gccgctgccc gcggccggaa cgggggacaa ggttgctgcc tggccgccgc gccgcggtcg
     8761 ctgggccatc gccctggagc gcgttgccct ggctgcggag cgcgtcgtca actacgtcac
     8821 taccgccaag tacaacccgc tctattacac gggcacaatc gccttcttcc tgttgctgat
     8881 cgtcgggctg accggcctgt atctgttcct cttcttccag tatggctacg atgcctccta
     8941 tgccgccgtc ggccgcatgg agtcgcagtt catcgcccgc accatccgcg ccatccaccg
     9001 ctacgcctcc ggggcgacag tgattgttac actgctgcat gcctatcgga tgctcttcat
     9061 ggagagattc cgcggccctc gttggttggc ctggctgacg ggcgtggcgc tgacgggcat
     9121 tgtttggttt gcgggcatca cgggctactg gctcatctgg gacgagcggg ctgtgctcat
     9181 caacgacacg ttcggccgcg ccctggcggc gctgaccccg tggcagcctg ctttcatggt
     9241 ctacctgagc ggggccgagg cggcggggcg gagctggatt ctctttctac tcgttctggc
     9301 tgtccacgtc atcctgtttc tcgtggccgt gggctttttc tatctccaca tcgtccggct
     9361 gaagcggcca aagtggctgc cgccgctgcc gtgggtcgcc ggcatcggcg tgattatgct
     9421 cctgagcggg ctgctattcc ccattggtat gttgccgcag ggagatttgc tgcgctttcc
     9481 gcaggcgatc accttcgatc ccatcttcct atttttcctg gcactgccgg gtggcccgtg
     9541 gctgtgggtg gcgctgctgg cagccacact ggtcacagca gcgctgccct ggctgtcgcg
     9601 gcggcagcgc gcggcggccg ggcgggcggc gaccttcccg ccggcgcacc ccgacgcgcc
     9661 gcgcgtgcga gtgctgggcg aacgctgcac gggctgtact ctctgcgcgc tggattgccc
     9721 ctatggcgcg ttggaaatgg tcgagcgcga cgacggccgc ccccacaagt acatcgcccg
     9781 cgaaatcgcc gatcgctgcg tctcatgcgc catttgcgtc ggctcctgcg atgtgctggc
     9841 catcacgctg ggcgaggcga tgcccgaaga tctgtggacg ctcatggctg ctggcggccc
     9901 ggccggcggc ggcccggccg ctggcggtcc ggctgcaacc tggtccggcg gcgatgccgg
     9961 cgacccaatt ggcctgaacg cgcgccggct ggccggggcg cggctcgtct tcacctgcga
    10021 gcgccacgcc gcccacggcg cgcgtgccta tctgaccggc gaccgggcag cggacgcgcc
    10081 gctggtggtc attcctctgc cctgcattgg cgcggccccg cccgacctgc tcagccgggc
    10141 gctggaggcg ggcgcggcca gcgtcagcgt tgtcggctgc ccaccggagg actgcgccaa
    10201 ccgcgagggc aacgcctggg ccgagcagcg cctgacgcgc cagcgcctgc cgcggctgcg
    10261 caagccctat atcgacgcgc cggtgacagc cgcctatctg ccgcccgacg cctttgccca
    10321 gggtctcaga gcgcttgcgc ccgcgaccgg gggcggcgcg cccgattacg ccgcctcgcg
    10381 cggcatgggg cggacgttct cgctgcgcca cttcggcccg gcgctggcgc tgctggcggt
    10441 ggcgctgctg ctgcaaatcc tgctgaccga cgtgcctttg cgcccggatg ccaaccggcc
    10501 ggctgtggtg caactggtca cgggcgatct cggcgcgacc ttcggcagcc tcgtcgccgg
    10561 acagacggcc gacagcgcgt accggttgga ggtgctggtc gatggtcggg aagtgttgcg
    10621 gcgcagcttc acggccgagg aagtagtggg gctggacagg gatgcgcgag cgcagttctt
    10681 ccacgagttg cccgtcgcgc cgggggcgca taccctgacg gtgcgcctga tcggcacgac
    10741 gaccggggcg acgttcgtcc tcttcgacga ccatgtgacg gtgagcgcgg gccaggcgac
    10801 gcagccggcg ctgtggccac agtcgctgtg gctcgacggg ccgcggccgt gcgtcagcga
    10861 ccacgcctgt gctcagtagc cttcggccag aaaagcgcga atccgcgcca gcagcacgtc
    10921 gggccggtcg cggtggatgg cgtggccgct gtcgctgacg aactctagcc gcgcgccggg
    10981 gatgcgctcg gccagccgcc gaacgtcgcg cggcgtgttg ccctcttcgc cgtcgccatt
    11041 gagcagcagc accggacagg caatgttggc cgcctgctgg taacagatgt tgccgccggc
    11101 ggcggcaatg gcctgcgcgg ccgccaccca gccctcaacc attgccggga actgctccgc
    11161 gccgtggcga gtgatgatct gctgcttcca ctgctctcgc tccggccccc aggcggtggc
    11221 gggcagccac ttggctaccc cggccagttc ctccggcgag atgacgccag agacccccca
    11281 ggcgaccacg ccgcgcacca gatcgggccg cgccgcggcc aacagcaacg ccacctcggc
    11341 tccgtcgctg aagccgagga cgacgaccgg gccaggttgc accgcgtcga gcagcgcggc
    11401 catgtcgacg gcgtcgcgtt cgtagaagtc gggcgggaag tcgcggttgg gcgggcggct
    11461 ctgaccatag ccgcgcaagt ccggggcaag gaggcgatgg gcaggggcga actcggcgat
    11521 caggttgccc aggtcggccg tggccgtgcc ggtgaagccg tggatgagca acagcggcga
    11581 gccgccggtc gggccggtgt cgatataggc caggcggtcg tgggtgggca agcgcaaaag
    11641 tggcatgggt gcaataccta ccctacatgc ggaaggtgcc atagccgccg ggcggcaagg
    11701 gcgcgtgagc cgtagcggcg taggccagcg ccaacgccgc gcgggtcttc accgggtcga
    11761 gaatgccgtc gtcccacagg cgcgccgtgg cgtagtaggg gctgctctcg cgagcgtact
    11821 gttcgaggat ggggcgcttg aacttctcct cggcggcagc gatctcgctg tcggttggct
    11881 cgtccatctg gtggaagacc tgcctcttgc ccaccgtcca gagcgtgtcc gccgcggcgt
    11941 cgccgctcat gacactgatg cggctattgg gccaggagaa gagaaagcgc gggtcatagg
    12001 cccggccgct catgccatag ttgcccgcgc cgtggctgac gccgtggagc agggtgatgc
    12061 gcggcacgtt gacgttgctg acggccatga ccatcttggc cccgtgcttg gcgatgcctt
    12121 cgttctcgta ttgccggccg accataaagc cggcgatatt ctgcatgaac agcagtggcg
    12181 tgccccgctg gccgcagagc tggatgaagt gggtcgcctt gagggacgat tcgctgaaca
    12241 gcaggccgtt gttggcaacg atgccgacgg ggatgcccag gatgtgggcg aagccgcaga
    12301 cgacggtcgg gccatagcgg gctttgaact cggttaggcg cgagccgtcc accagccggg
    12361 cgatgatctc gcgcacgtcg tagat
//
LOCUS       DAOSMQ010000019        12045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5265_length_12045_cov_4.487323, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000019 DAOSMQ010000000
VERSION     DAOSMQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12045
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5265_length_12045_cov_4.487323"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK829_01300"
     CDS             <1..301
                     /locus_tag="PK829_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39878.1"
                     /translation="RPFALAVLMPAAVARAVAAFAAEWPFSAAPSAWACLSFMDNNRN
                     PGQFAFSLCIYPALAGSALLVRRLPAVPGPYLFLSSTEYILNNIQCIFNTPIGLS"
     gene            538..2883
                     /locus_tag="PK829_01305"
     CDS             538..2883
                     /locus_tag="PK829_01305"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU39879.1"
                     /translation="MRLNETIAHYFSADALADLAEFIGFRPVAPPAASATPAVAPAAE
                     STALLAESTALLAEAAARGRIAALLAALAARVPTVDWAALPWPPAALAALHDALDRRL
                     GREDLRDLCLCLGVDYDDLPGEGKRARARELVLRMARTGRLVELEAEANAWTQRGKGA
                     KRKQGARARRGVRVGGAAKGLPLRWAVAGLLVVCLVAVALVWRAGRGWVSGPALPEDV
                     VGVAVAEFAETADCHRGARGREASALVYETLGRELAAAGLARRVGLTRVGRVCAAAEA
                     VAAGQAVGAEVVVWGWLPSSEGLVATFTPTAAAPAASADLARAFEALFTGPDEALSLR
                     LSGRALVLTRFLLGLVRAGQGEPAAAQALYDAAIVAVEAELATAGSGEGAELRRTLAV
                     LLTERGKARAALGRPDDARADYDRAEALNPDYLRLLIARAAEAYGRRDWAAAERYLDR
                     AALQDEPLPTIAYGYGLLDYYRGRYASAVATFDRAIALSAADGSDAAIYYLARGYSYV
                     ELGDCAAAAADFRAAGSDANAPAAVRRAAASAGCDAAAVAGTGGQPAGAPSAAPGEAV
                     TPTPDGTPAVALSGIAPTRLSPLEMAAAAPTRADATPATTPTATAPALATPGPLLVEV
                     GPRGANVRQGPGAEYPVMATRRAGETLEVLAANASGEWLQVRVPGQGRGWVSAAYAAA
                     LGDVSGLAVGYYQPGIIIAGAGAATTGAFATPTPARPQPAARAQPSATPAYTPPGPSP
                     TPPPPPDGPAPTRTPPAVSTSPAGWWPTPTATLLPAPTARR"
     gene            complement(3051..3467)
                     /locus_tag="PK829_01310"
     CDS             complement(3051..3467)
                     /locus_tag="PK829_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU39880.1"
                     /translation="MITYRRDAPLTADDYIALLRRSTLAERRPVDDAAAIAGMVANAG
                     LTVTAWDGELLVGVARSVTDFTYCCYLSDLAVDVAYQRQGIGVELIARTQAALGPRCN
                     LILLAAPAAADYYGHIGFVHHSRCWILPRDRRPGRP"
     gene            complement(3464..4261)
                     /locus_tag="PK829_01315"
     CDS             complement(3464..4261)
                     /locus_tag="PK829_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020329668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU39881.1"
                     /translation="MDTLELIHHHRSIRRYRPDPIPDDLLTEVLAAGVRASSSGNMQT
                     YSIIVTRDRALREQLYEPHMEQSMVLDAPALLTFCADFRRMRHWLRLSAAPDNFDNFM
                     SFLIAAIDATLVSQNVALAAEARGLGLCYMGSTLANCDQIGRVLQLPPGVFPVVGYSL
                     GWPDEDPAPRDRLPLSGLIHNETYHDYSDDDIRAIYRERETRGWQRYMSYPELRLMIE
                     ASGVQNLAQVYTVVKYTRESHEAFSQTVLDYLRQQGFLEQQLAEAQP"
     gene            complement(4342..5361)
                     /locus_tag="PK829_01320"
     CDS             complement(4342..5361)
                     /locus_tag="PK829_01320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU39882.1"
                     /translation="MSRPASERIAVPGAPAVAGLVFRSFRGESDYAAMVRLANERAAA
                     NGDDWNLTVAELAADFAPTAHFNPAADALLAEVEGTLAGFWRGEWRAENDGPLIYRIE
                     HFLHPRWRGQGIGRAALQWTEDRMRAIAATHDPARPKILQGFASHGNRYQAELLEANG
                     YQAVRHFHLMVRPALDDIPDFPLPEGLEVRPVRPEHYRALWDADVEAFHDAWGVSDPD
                     EAEYQTWLDNKIWFQPELWQVAWDPAVERVAGQVRTFIDHGENERFGRLRGYTEFISV
                     RRAYRRRGLARALIARSLRAQRDAGMTASALHVDAESLTGAPRVYEDCGFRVQTTDTL
                     YRKAL"
     gene            complement(5373..6047)
                     /locus_tag="PK829_01325"
     CDS             complement(5373..6047)
                     /locus_tag="PK829_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HOU39883.1"
                     /translation="MQEHVQATDQRDDNTVEFQDTTLPLSETLRGVARAVTGDRITTR
                     ELLALAGEQGMLLALMVLTLPFLFPVSIPGVSTVFGLVGILISIGVTLNRVPWLPDRL
                     LDRPLGTEKLVPAIEKGADAFRRVDRISHPRLQRLTDGRAVSRVNGLGLLAASILLLF
                     PLSFVPFSNTLPAVAMLLLAAGMLQRDGVLVLLGYLFLLATVIYFAALFIGAVMTGQG
                     IMGLFR"
     gene            6368..7129
                     /locus_tag="PK829_01330"
     CDS             6368..7129
                     /locus_tag="PK829_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOU39884.1"
                     /translation="MANLATTHYWDQSPYQLRCEWGPRGVAALASQCDVVVIVDVLSF
                     STCVDIAVARGAIVYPAGPGLESPADMAHRLGAAVVGRDASAAYVFSPTGMLAVPAGA
                     RLVLASPNGATLSTLTGATPTLAGCLRNARAVAAAAARLGRRVAVIAAGERWREDAHG
                     DRSLRPGVEDLVAAGAILDRLPGSRSPEAEVAVAAFRAVRGRLADVLLACSSGRELVA
                     IEREADIPLAAQFNVSDFAPRLIDGAYRAAAGEAP"
     gene            7126..8076
                     /locus_tag="PK829_01335"
     CDS             7126..8076
                     /locus_tag="PK829_01335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU39885.1"
                     /translation="MIRVIRCHPNRFDALVDFVAAYNPLPEHHIGYFGLTPADIRQEL
                     RHFSLRFDRATSIAVEGGRIVGAFGADVDAEIGRAWLFGPLVAAQGAAERAAEPWATT
                     ADALYAAVAARLPAAVREHQMFVDSRNVRAAAFAGRHGFAPAGEWAIYFLPAGRLDAL
                     PAAEAQPWDARYNDQLDALHNRLFPSSNYTLGYILREWAERGAILLAAAEGDTLHGYF
                     FGRVEAETGEATVDLVGVDETRRRAGLGRRLMLAGLARMRGAPGLRQVNLSVAAANTP
                     ARTLYDDLGFLWERDMAAYQRALTSASLPSNISRRMHDDR"
     gene            8063..8506
                     /locus_tag="PK829_01340"
     CDS             8063..8506
                     /locus_tag="PK829_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU39886.1"
                     /translation="MTTDNPLPEPTLEVVDTADPADADFVHAGLRAHNAQFVAEDWRR
                     LYVFLRDAGGAIVGGLLGDTFWGWLAVHILWIDARYRGQGYGDRVLRAAEDEARARGC
                     RHVQLDTLSFQARPFYEARGYRLYGQLEDFPAGSGHTRYYLTKPL"
     gene            8527..9774
                     /locus_tag="PK829_01345"
     CDS             8527..9774
                     /locus_tag="PK829_01345"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU39887.1"
                     /translation="MTPKRVLILTADAGFGHRSTANALAAALARRYGDAVAATIVNPL
                     DEPGAPPLMRSAESDYDKLATQWPGFYKLGYKMSDLAMTTSVTEMTYVLLMYDLIARL
                     IKQHQPDAVVVTFPTYQYPVAAYRRLSGARVPMATIVTDLVSLQKMWFTPSADLCLVP
                     TPFAADLALERGLDPETVHVTGLPVNPALADAPPKAEARAALGWPAGKFTVLAVGSKR
                     VEGLDQFLHVLNHSGFDIHLVAVAGGNDELYARLRAAEWHIPATIYNYVSDMPSLLHA
                     ADCVISKAGGLIVTESLACGLPLLLMQVIPGQETGNAALVTREGAGALTPEPLLLLEA
                     LADWLADGRRLYRERAANARRLGRPDAAYAAADLIWEACRHRLPDPSRRFPISRAKLK
                     ALLQSFGDALSLGDWSPDDRPLE"
     gene            9771..11195
                     /locus_tag="PK829_01350"
     CDS             9771..11195
                     /locus_tag="PK829_01350"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOU39888.1"
                     /translation="MSAPAFPRGFRFGAATSAYQIEGSPAAEGKGLSIWDRFTRRPGR
                     VYRGHTGNVAADTYRDFQPDVAILRQLGLNAYRFSVAWPRVLPEGRGAVNTRGLDYYD
                     RLVDALLAADVTPFVTLFHWDLPDALQRTIGGFAARDCAALFADYAEVVAARLGDRVR
                     HWITLNEPWVFATLGHLRGLHAPGRHNPWAFLRVVHHQLLAHALAAPRLRARGDAARV
                     GLSLNLSPVYPLTGSDADRAAARLADAGVNRLFLDPLCRGYYPQELWTRLRRFHPPVQ
                     PGDMEAIRGSLDFVGVNYYSRVRVRFRRLVPYLQLWPDGLPLAGHAATLHPRGAAEQS
                     AMGWEVYPAGLFELLFRLKQEYGNPPVFVTENGAAYDDPPPVNGRVPDEARRRYLAQH
                     LDIVAAALRAGADVRGYFVWSLIDNFEWTYGYDKRFGLVYVDFATQQRTIKDSGHWYG
                     AFARQQTAASGPRGTSAPRPASIE"
     gene            11234..11893
                     /locus_tag="PK829_01355"
     CDS             11234..11893
                     /locus_tag="PK829_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39889.1"
                     /translation="MRKWFVMLCLLAAALLPVACAPAGGQEPAVVVEPTIDAPALNPV
                     PLPAETDPEVNAAEAAPAGDAAGNTASDETLAAPPATTRPSSAEPVSPGEVALGDLTP
                     TTPSAPRREAPAPGRPAADSPAGRERVMVVTAVLDMIDRLGVSAESVAVESIEAITWP
                     DASLGCPQPGVDYAQVAIAGSRITLRAGDETFTYHTDGDSRYILCQDGEVVDEGVAPG
                     R"
BASE COUNT         1644 a         4380 c         4203 g         1818 t
ORIGIN      
        1 gcggccgttc gccctggctg tgctcatgcc agcggccgtg gcgcgggccg tggctgcctt
       61 cgcggccgaa tggccgttct cggccgcccc gtccgcgtgg gcctgcctgt ccttcatgga
      121 taataatcgt aatcctggtc aattcgcatt ctccttatgc atctatccgg ccctggccgg
      181 ttcggccttg ctggtacgcc ggttgcccgc cgtgcccggc ccttatctct ttctttcaag
      241 tactgaatac atattaaaca atattcaatg tatattcaat acacctatcg gtctctctta
      301 actggcattc atctagccac aagaagaccc gtggcggcca ggccttgctc tcagagcgaa
      361 cgcgcatcta aggaggcggc cggggggata agcccctgtg tcagacgccc tctctcgtgc
      421 ccttgctccc ctgcgccccg tgctgtaatg ctccttgtaa ccttcccctc ctaccctaaa
      481 agctgggaag tcagtcgccc ggccgccggc agccgtaacg ccggcaggac acggacaatg
      541 agactgaacg agaccatcgc ccattacttt tcggcggatg cgttggccga cctggctgag
      601 ttcattggct tccggcccgt cgcaccgccc gccgcttcgg ccactccggc ggtcgccccc
      661 gcggccgagt ccacggctct cctggccgag tccacggctc tcctggccga ggccgccgcc
      721 cgcggacgca tcgctgccct tctggctgcc ctggctgccc gcgttcccac tgtcgattgg
      781 gccgcgctgc cctggccgcc ggccgcgctg gccgccctcc atgacgccct cgaccgccgt
      841 ctgggccgcg aagatttgcg cgacctctgc ctgtgcctgg gcgtggacta cgacgacctg
      901 cctggcgagg gcaagcgcgc ccgcgcccgc gagctggtgc tgcggatggc gcggacgggg
      961 cggctggtgg agttggaggc agaagcgaac gcctggacgc aaagaggcaa gggcgcaaag
     1021 aggaagcaag gggcgcgggc gcggcggggt gtgcgggttg gcggcgcggc aaaagggttg
     1081 ccgctccgct gggctgtagc ggggctgctg gtggtatgtc tggtagccgt ggctctggta
     1141 tggcgggcgg ggcggggctg ggtgtccggg ccggcgctgc cggaggacgt cgtcggcgtg
     1201 gccgtggccg agttcgccga gacggccgac tgccaccgcg gcgcgcgcgg ccgcgaggct
     1261 tcggcgctgg tctacgagac gctgggccgc gagctggccg cggccggcct ggcgcggcgt
     1321 gtcggcctga cccgcgttgg ccgtgtctgc gcggcggccg aagccgtcgc cgcggggcag
     1381 gctgttgggg ccgaggtcgt cgtctggggc tggctgccat cgtctgaggg cttggtggcc
     1441 accttcacgc ccaccgcggc cgcgccggcg gcctcagccg acctggcccg cgccttcgag
     1501 gcccttttca ccgggccaga cgaagctctt tcgctacgtc tttctggccg ggcgctggtg
     1561 ctgacccgct tcctgctcgg cctggtgcgc gccgggcagg gtgagccagc cgcggctcaa
     1621 gccctctacg acgccgccat tgtagctgtg gaagcagaac tggccacggc tggatcgggc
     1681 gagggcgcgg aactgcgccg cacgctggcc gtgctgctga ccgaacgcgg caaggcccgc
     1741 gccgccctgg gccgcccgga cgacgcccgc gccgactacg accgcgccga ggcgctcaac
     1801 cccgactacc tgcgcctgct gatcgcccgc gccgccgagg cctacggccg ccgcgactgg
     1861 gccgcggccg agcgctatct cgaccgcgcc gcgctgcaag acgagccgct gccgaccatt
     1921 gcctacggct acggcctgct cgactactat cgcgggcgct acgcctcggc cgtggccact
     1981 ttcgaccgcg ccatcgccct gagcgccgcc gatggcagcg acgcggccat ctactacctc
     2041 gcccgtggtt atagctacgt cgagctgggc gactgcgccg ccgcggcggc cgacttccgc
     2101 gccgcgggca gcgatgccaa cgcgccggcc gccgtgcgcc gggcggcagc atcggccggc
     2161 tgcgacgcgg ccgccgtcgc cggcacaggc ggccagcctg ccggcgcgcc gagcgctgcg
     2221 ccgggcgaag cagtcacccc cacgcccgac ggcacgcccg cggtggcgct gtcgggcata
     2281 gcgcccacgc gcctctcgcc gctggagatg gccgccgccg ccccgacgcg cgccgacgcg
     2341 acgccggcca ccacgcccac agccacagcg cccgcgctgg ccacgcccgg cccgctgctg
     2401 gtggaggttg gcccgcgcgg agccaacgtg cgccaggggc cgggggcgga atacccggtc
     2461 atggccacgc ggcgggccgg cgagacgctg gaggtgctgg cggccaacgc cagtggagag
     2521 tggttgcagg tgcgcgtgcc ggggcagggg cgggggtggg tgtcggctgc ctacgccgcg
     2581 gctctgggcg acgtcagcgg gctggcggtg ggctactacc agcctgggat catcatcgcc
     2641 ggggctggcg cggcgacaac cggggccttc gccaccccca cgccggcccg gccgcagccg
     2701 gccgccaggg cacagcccag cgccacgccc gcctacacgc cgcccgggcc gtcgccaacg
     2761 ccgccaccgc cgcccgatgg ccccgcgccc acgcgcaccc cgccagcagt gtccacgtcg
     2821 ccggcgggct ggtggccaac gcccacggcc acgctgctgc ccgcgccaac cgcgcgccgc
     2881 taggcccggc ggcgcgttca cgccttatcc acgtcatatc caccgcgcct taacgccgcg
     2941 gcgcgcgggc atcggctatc ctacaggtgg gcagcagtac agttttcctc ctttttgaga
     3001 cgccgcgatt tccccttttc gcggcgtttc ccttttctgg ggaggagcgg ctacggccgg
     3061 ccgggccgcc ggtcgcgggg caaaatccag cagcgcgaat ggtggacaaa gccgatgtgg
     3121 ccatagtagt cggccgccgc cggggcggcc agcaggatga ggttgcagcg cgggccgagc
     3181 gccgcctgcg tccgggcgat cagttccacg ccgatgccct gccgctggta ggccacgtct
     3241 acggccagat cggacaggta gcagcagtag gtgaaatcgg tcaccgagcg ggccacgccc
     3301 accagcaact cgccatccca ggccgtgaca gtcaggccgg cgttggccac catgccggcg
     3361 atggccgcgg cgtcgtccac cggccggcgc tcggccaggg tggagcggcg cagcagggca
     3421 atgtagtcgt cggcggtgag gggcgcgtcg cgccggtagg tgatcatggt tgcgcctccg
     3481 ccagctgttg ctccaggaag ccctgttggc gcagatagtc gagcaccgtt tgcgagaacg
     3541 cctcgtgcga ttcgcgggtg tacttgacga cggtgtagac ctgggccagg ttctgcacgc
     3601 ccgacgcctc gatcatcagc cgcagctccg gatagctcat gtagcgctgc cagccgcgcg
     3661 tctcccgctc gcggtagatc gcgcggatgt cgtcgtcgct gtagtcgtgg taggtctcgt
     3721 tgtggatgag gccgctcagc ggcaggcggt cgcgcggggc ggggtcttcg tctggccagc
     3781 cgagcgagta gccgacgacg gggaagacgc ccggcggcag ttgcaggacg cggccgatct
     3841 ggtcgcagtt ggccagcgtg ctgcccatgt agcacagccc caggccgcgc gcctcggccg
     3901 ccagggcgac gttctgcgac accagcgtcg cgtcgatggc ggcgatcagg aagctcatga
     3961 agttgtcgaa gttgtcgggc gcggcggaca ggcgcagcca gtggcgcatg cggcggaagt
     4021 cggcgcagaa ggtcagcagc gccggcgcgt cgagcaccat gctctgttcc atgtgcggct
     4081 cgtagagctg ctcgcgcagc gcccggtcgc gggtgacgat gatggagtag gtctgcatat
     4141 tgcccgacga ggaggcgcgc acgccggcgg cgagcacttc ggtcagcagg tcgtcgggga
     4201 tggggtcggg ccggtagcgg cggatggagc ggtggtgatg gatgagttca agggtgtcca
     4261 tgcccgccaa gtatgcccca attggcccgc ggcgggaatg gccgggcgtt ttttctccgc
     4321 gttcccgctg gatgggtaag gctacaacgc cttgcgatag agcgtatccg tcgtctgcac
     4381 gcggaagccg cagtcctcat agacacgcgg cgcgccggtc agactctcgg cgtcgacgtg
     4441 cagggcggaa gcggtcatgc cggcgtcgcg ctgggcgcgc aggctgcggg cgatgagcgc
     4501 gcgcgccagc ccacggcggc ggtaggcgcg gcgaacgctg atgaactccg tgtagccgcg
     4561 caggcggccg aagcgctcgt tctcgccgtg gtcgatgaag gtgcgcacct gcccggccac
     4621 gcgctccacc gccgggtccc aggccacctg ccacagttcc ggctggaacc agatcttgtt
     4681 gtccagccag gtctggtact ccgcctcgtc cgggtcgcta acgccccagg catcgtggaa
     4741 cgcttccacg tcagcgtccc agagggcgcg gtagtgctcc ggccggacgg gccgcacctc
     4801 cagcccctcg ggcaggggga agtcggggat gtcgtcgagc gccggccgca ccatcagatg
     4861 gaagtggcgt acggcctggt agccgttggc ttccagcaac tcggcctgat agcgattgcc
     4921 gtgggaggcg aaaccctgga ggatcttcgg ccgcgccggg tcgtgggtcg cggcgatggc
     4981 gcgcatgcgg tcctccgtcc attggagcgc cgcgcggccg atgccctgcc cgcgccagcg
     5041 ggggtgcaga aagtgctcga tgcggtagat gagcgggccg tcgttctctg cccgccactc
     5101 gccgcgccag aagccggcca gcgtcccctc tacctcggcc agcagggcgt cggcggcggg
     5161 gttgaagtgg gcggtgggcg caaagtcggc ggccagttcc gcgacggtca ggttccaatc
     5221 gtcgccgttg gccgcggcgc gctcgttggc caggcgaacc atcgccgcgt agtcgctctc
     5281 gccgcggaac gagcggaaga ccagcccggc aacggccggc gcgccgggaa cggcaatccg
     5341 ttcagaagcg gggcggctca cggtcgtgcc ttctagcgaa acagccccat gatgccctgg
     5401 ccggtcatga ccgcgccgat gaacagagcg gcgaagtaga tgaccgtggc cagcaggaac
     5461 aggtagccca gcaagaccag cacgccgtcg cgctgcaaca tgcccgcggc cagcagcagc
     5521 atggccaccg ccggcagggt gttggagaag ggcacaaagc tgagcgggaa gagcagcagg
     5581 atgctggccg ccagcaggcc caggccattg acccggctca ctgcccggcc gtcggtcaat
     5641 cgttgcagcc gcgggtggct gatgcgatcc acgcggcgga aagcatcggc ccccttttcg
     5701 atggccggaa cgagtttctc cgtgcccagc ggccggtcga gcagccggtc gggcagccag
     5761 ggcacacggt tgagcgtgac gccgatggag atgaggatgc cgacgaggcc aaagaccgtg
     5821 ctgacgccgg ggatcgatac cgggaacagg aacggcagtg tcagtaccat cagggccagc
     5881 aacatcccct gttcgccggc cagagccagc agctcgcggg tagtgatgcg gtcgcccgtg
     5941 acggcgcggg ccacgccgcg cagtgtctca ctcaggggca gcgttgtgtc ctggaactcg
     6001 acggtgttat cgtcgcgttg gtcggttgcc tgaacgtgtt cttgcatctt tcttcctgat
     6061 ggcgcggccc tcggccggtc gccggttcga tagtgcccga ccgccggagt gggccaggcc
     6121 aacaatcacc cagtgtagcc gccaccgtgg cgcttgacca gtggcaaacc atgatttcac
     6181 ccgccgttca cctggcggta ggggcgcgcc gacctgaccg gtgggcggcc acgagtggca
     6241 ctgccaccag agaacgacgg gccgcccacc ggtcaggtca atggtcgtta gacgactttg
     6301 gcaaagccat ggggccgaca cgccggtctg cccctaccca gcgccatcat ggtataatcc
     6361 ccaccttatg gccaaccttg cgacgaccca ttattgggat caatctccct atcaactgcg
     6421 ctgcgagtgg gggccgcgtg gcgtggcggc gctggcctcg cagtgcgatg tcgtcgtcat
     6481 cgtcgatgtg ctgtctttct ccacctgtgt cgatatcgcc gtggcccgcg gggcgatcgt
     6541 ctatcccgcc gggccgggcc tggagtcgcc ggccgacatg gcccaccgcc tgggcgcggc
     6601 cgtcgtcggg cgagatgcgt cggcggccta cgtcttctcg cccacgggca tgctggctgt
     6661 gcccgccggg gcgcggctgg tcttggcttc gcccaacggg gccacgctga gcaccctgac
     6721 cggggcgacg ccgacgctgg ccggctgcct gcgcaatgcc cgcgccgtgg ctgccgccgc
     6781 cgcccgcctc ggccggcgcg tggccgtcat cgccgccggc gaacgttggc gcgaggacgc
     6841 ccacggcgac cgctcgctgc ggccgggcgt ggaggatctc gtcgccgccg gagccatcct
     6901 cgaccgcctg ccgggcagcc gctcgccgga ggccgaggtg gccgtggccg ccttccgcgc
     6961 cgtccgcggg cggctggcgg acgtcttgct ggcgtgcagt tccggccgcg aactggtcgc
     7021 catcgagcgc gaggccgaca tcccactggc cgcccagttc aacgtcagcg acttcgcccc
     7081 gcgcctgatc gatggggcct accgcgccgc cgccggagaa gcgccgtgat ccgcgtcatc
     7141 cgctgccacc ccaaccgctt cgacgccctg gtggacttcg tcgccgccta taaccccctg
     7201 ccggagcacc acatcggcta cttcggcctg acaccggccg acatccgcca ggagttgcgc
     7261 cacttcagcc tgcgcttcga ccgggccacc agcatcgccg tcgagggcgg gcgcattgtt
     7321 ggcgcgttcg gggccgacgt tgacgcggag atcggtcgcg cgtggctctt cgggccgctg
     7381 gtggccgctc agggggccgc tgagcgagcc gccgagccgt gggcaacgac ggccgacgcc
     7441 ctctatgctg ccgtcgccgc ccggctgccg gcagccgtcc gcgagcatca gatgttcgtc
     7501 gatagccgca atgtccgcgc cgcggccttc gccggccgcc acggcttcgc gcccgccggc
     7561 gagtgggcca tctacttcct gcccgccggc cggctggacg ccctgcccgc ggccgaggcc
     7621 caaccgtggg acgcccgcta caacgaccag ctcgacgccc tgcacaaccg cctcttcccg
     7681 tcgtccaact acaccctcgg ctatatcctg cgcgaatggg ccgagcgcgg ggccatcctg
     7741 ctggccgcgg ccgagggcga cacgctccac ggctacttct tcggccgcgt cgaggcggag
     7801 acgggcgagg ccactgtcga tctcgtcggc gtggacgaga cgcgccgccg cgcgggcctg
     7861 ggccggcggc tcatgctggc cgggctggcg cgcatgcgcg gcgcgcccgg cttgcgccag
     7921 gtcaatctca gcgtcgccgc cgccaacacg ccggcccgga cgctttacga cgacctcggc
     7981 ttcctgtggg agcgggacat ggccgcttac cagcgggcgc ttaccagcgc gtcgcttccc
     8041 agcaacatct caaggaggat gcatgacgac cgataacccc ctgcccgaac cgacgctgga
     8101 ggtcgtagac acggccgacc cggccgacgc cgatttcgtc cacgccggcc tgcgcgccca
     8161 caacgcgcag ttcgtggccg aggactggcg gcggctgtac gtcttcttgc gcgacgccgg
     8221 cggagccatc gtcggcgggc tgctgggcga taccttctgg ggctggctgg ccgtccacat
     8281 cctgtggatc gatgcgcgct accgcggcca gggctatggc gaccgcgtcc tgcgggcggc
     8341 cgaggacgag gcccgcgccc gcggctgccg tcacgtccag ctggacacgc tcagcttcca
     8401 ggcgcggccg ttctacgagg ctcgcggcta ccggctctat ggccagctgg aggactttcc
     8461 cgccggctcc ggccacacgc gttattacct gaccaagccg ctctagggcc gcccacccga
     8521 ccgcccatga cccccaagcg cgtcctcatc ctgaccgccg acgccggctt cggccaccgt
     8581 agcacggcca acgccctggc cgcggccctc gcccggcgct atggcgacgc cgtcgccgcg
     8641 accatcgtca acccgctcga cgagccgggc gcgccgccgc tgatgcgcag cgccgagagc
     8701 gactacgaca agctggcgac gcagtggccc ggcttctaca agctcggcta caagatgtcc
     8761 gacctggcca tgaccacgag cgtcaccgaa atgacctacg tgctgctgat gtatgacctc
     8821 atcgcccggc tgatcaagca gcaccagccc gacgccgtcg tggtcacctt cccgacctac
     8881 cagtaccccg tggccgccta ccgccgcctc agcggcgcgc gcgtgcccat ggccaccatc
     8941 gtcaccgacc tcgtgtcgct gcaaaagatg tggttcacgc cctcggccga tctctgcctg
     9001 gtgcccacgc ccttcgccgc cgacctggcc ctggagcgcg gcctcgaccc ggagacggtg
     9061 cacgtgaccg ggctgccggt caacccggcg ctggccgacg cgccgcccaa ggccgaggcg
     9121 cgggcggcgt tgggctggcc ggccggcaag ttcaccgtcc tggccgtggg cagcaagcgc
     9181 gtcgaggggc tggatcagtt cctccacgtg ctcaaccatt ccggcttcga catccacctg
     9241 gtcgccgtgg ccgggggcaa cgacgagcta tacgcgcggc tgcgggcggc cgagtggcac
     9301 atcccggcga cgatctataa ctacgtcagc gacatgccca gcctcctcca cgccgccgat
     9361 tgcgtcatca gcaaggccgg cgggctgatc gtcaccgagt cgctggcctg tgggctgccg
     9421 ctgctgctca tgcaggtcat ccccggccag gagacgggca acgccgccct ggtgacgcgc
     9481 gagggcgccg gcgcactgac gccggagccg ctgctgctgc tggaggcgct ggccgactgg
     9541 ctggccgacg gccgacgcct ctaccgcgag cgggccgcca acgcccggcg gctgggccgg
     9601 ccagacgcgg cctatgccgc cgccgacctc atctgggagg cgtgccgcca tcgcctgccc
     9661 gaccccagcc gccgcttccc catctcccgc gccaagctga aggcgctgct gcaatcgttc
     9721 ggtgacgcgc tgtcgctggg cgactggtcg ccggacgacc ggccgctgga atgagcgcgc
     9781 ccgccttccc gcgcggcttc cgcttcggcg cggccacctc ggcctaccag atcgagggca
     9841 gcccggcggc cgagggcaag ggcctgtcca tctgggatcg tttcacccgc cgccccggcc
     9901 gtgtctaccg cggccacacc ggcaacgtgg ccgccgatac ctaccgcgac ttccagcccg
     9961 acgtcgccat cctgcgccaa ctggggctga acgcctaccg cttctccgtc gcctggccgc
    10021 gcgtgctgcc ggaggggcgc ggggcggtga acacgcgcgg gctggactac tacgaccggc
    10081 tggtggacgc cctgctggcc gcggacgtca cgccgttcgt gaccctgttc cactgggact
    10141 tgcccgacgc gctgcaacga actatcggcg gcttcgccgc gcgcgactgc gccgcccttt
    10201 tcgccgacta cgccgaggtg gtcgccgcgc ggctgggcga ccgggtgcgc cactggatca
    10261 ccctcaacga gccgtgggtc ttcgccacgc tggggcatct gcgcgggctg cacgcgccag
    10321 ggcggcacaa cccgtgggcc ttcctgcgcg tcgtccacca ccagttgttg gcccacgcgt
    10381 tggccgcgcc ccgcctgcgc gcccgcggcg acgccgcccg cgtcggcctg agcctcaacc
    10441 tgtcgcccgt ctatcccctg accggcagcg acgccgaccg cgccgccgcc cggctggccg
    10501 acgccggggt caatcgcctc ttcctcgacc cgctctgccg cggctattac ccgcaggagc
    10561 tgtggacgcg cctgcgccgc ttccacccgc ccgtccagcc cggcgatatg gaggcgatcc
    10621 gcggctcgtt ggacttcgtg ggcgtcaact actacagccg cgtccgcgtg cgcttccgcc
    10681 ggctcgtgcc ctatctgcaa ctctggcccg acgggctgcc cctggccggc cacgcggcca
    10741 cgcttcatcc ccgcggcgcg gccgagcaga gcgccatggg ctgggaggtc tacccggcgg
    10801 ggctgttcga gctgctattc cgtctgaagc aggagtacgg caacccgccg gtcttcgtca
    10861 ccgagaacgg cgcggcctac gacgacccgc cgccggtgaa cggccgcgtg cccgacgagg
    10921 cccgccgccg ctacctggcg cagcacctcg acatcgtcgc cgcggcgctg cgggccggcg
    10981 cggacgtgcg cggctacttc gtctggtcgc tgatcgacaa cttcgagtgg acttacggct
    11041 acgacaagcg cttcggtctg gtctacgtcg atttcgccac ccagcagcgc accatcaagg
    11101 acagcggcca ctggtatggc gcgtttgccc gccaacagac ggccgcctcc ggccccaggg
    11161 gaacatccgc gccccgtcca gcgtccattg aatagcggtg gcgcctgatc gccggccgcg
    11221 tggaggattg tcgatgcgaa agtggtttgt gatgctgtgt ctgctggccg cggcgctgtt
    11281 gcccgtcgcc tgcgcgccgg ctgggggcca ggagccggct gtcgtcgttg agccgacgat
    11341 tgacgccccg gccctgaacc ccgtgcccct gccggcggaa accgatcccg aagtgaacgc
    11401 ggccgaggcc gcgccggccg gcgatgccgc cggcaacacg gcgagcgatg agacgctggc
    11461 cgcgcccccg gcgaccacgc gcccatcttc ggcggagccg gtctcgccgg gcgaggtggc
    11521 gttgggcgat ctgacgccga cgacccccag cgccccgcgc cgcgaagccc ccgcgcccgg
    11581 ccgccccgcg gccgattcgc cggccgggcg cgagcgcgtc atggtggtga cggccgtgct
    11641 ggatatgatc gaccggctcg gcgtgtcggc cgagtcggtc gcggtcgagt ctatcgaggc
    11701 gatcacctgg ccggatgcga gcctgggctg cccccagccg ggcgtggact atgcccaggt
    11761 ggccatcgcc ggcagccgca tcaccctgcg ggccggcgac gagaccttca cctaccacac
    11821 cgacggcgac agccgctaca ttctctgcca ggatggggag gtggtggatg agggggtggc
    11881 accgggtagg tagtgggcag taagcagtaa gtagtaagca gtaaacagta aacagtaaac
    11941 agtaggtcag tattcagcgg cagtcggcgg tcagcggtcg gccgtccgtc gtcggcggtc
    12001 agcggtcggc cgtccgtcgt cggcggtcag cggtcggccg tccgt
//
LOCUS       DAOSMQ010000020        11900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5332_length_11900_cov_5.127649, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000020 DAOSMQ010000000
VERSION     DAOSMQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11900
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_11900_cov_5.127649"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PK829_01360"
     CDS             <1..1380
                     /locus_tag="PK829_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU39890.1"
                     /translation="FGVGYRDEARTLRTLFVVPDDSPMRPFVEDYSTRLGRQIDPRGI
                     IPDAAEADRELRQQQVDLVVVVPPDPAAQLNNNEQATFQMYHYEIDPYEQTYIEILGR
                     NYIEEINRQVLLTAADQGKVEAAALQERVTAARETAAAARVALEAGDEAAAGIAAGDL
                     DSQLGLLAVAAGSGLALLGGVENTLGAETLQAQLLAIQAASAALSADTTTPVAERAAS
                     AAAIEADLAEVDGVLSRYQGIDSQVLVSPFRSETLSITDVSLGPTDFFVPAVIALLLQ
                     HIAVTLAGLSIVRERQDGPLELFRASPVSAFQTLIGKFIGYLILTAVIGAVLTALVVF
                     GLGVPMLGNWGYYALVVLALLCASLSLGFFLSLLAHTDSQAIQYAMLLLLASIFFSGF
                     FLALYRLWEPVRAVSWALPVTYGINLLQSVMLRGQMPGMIMLGALFLFALVFFVLDWW
                     RLSRLMARQ"
     gene            1412..2701
                     /locus_tag="PK829_01365"
     CDS             1412..2701
                     /locus_tag="PK829_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU39891.1"
                     /translation="MLDHRPALELPELSVRQLVTATLVVGLVALSIWLVFRFNQVILI
                     LIAGIIISTALQPVVNWLQRRGLPAGAAVLLLFGVLALIVGLFLRFGVPLIADQAANI
                     GAQLGDVYAGFVQRLGQSSNLLIRRLAEVIPPQMGLPANVPAAPVEGQGVEVVESPFA
                     QVWLTLGTVARVIYKTAAIFFVAFFWTVESDRIKRVGIALLPLNRREPIRDLITEIEG
                     RVGGYVSGQVLVSGIIGTLSLIAYLIIGIPYALALALVIAVMEFIPVVGPLIGAIPSI
                     IIALSIAPSKALWVLLTSLIIHQLEANFFGPRVMKRTLGMRPLVTLVAMTAFGTLFGI
                     LGALIALPLTAIIQMLLERFMLSNTPQTEPTGERDRIGLLRYQTMELVEDVRKVLRQR
                     ENAPPDEEGTHLEDVIETIALDLDKLLSQSRNGAEAS"
     gene            2698..3879
                     /locus_tag="PK829_01370"
     CDS             2698..3879
                     /locus_tag="PK829_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU39892.1"
                     /translation="MKRLAYYVLIIAVTVAMLILLWQFRSVLLLLLLSLMLAAVLRPS
                     AEFLKSRGLPVSLSWLLVYLGLFAVVGLSIYLLGGHLLDELQKLSNYLVILYDFTYRS
                     WSGGSGIQQAIVQRIPPPDQLPEVMAGPSGLSILRLIFGVGQNVVTVGASLLIVIVLS
                     LYWSTDREHFERLWLSLLPPGKRVPARSIWQTAEASIGNYLRSELVQALLAVVLLAVG
                     FRLLGLDYPLLAALLAGLFWLVPMAGFVFAAVVAFLAGMATNGHVQTALFALVLTAGV
                     LAFLEFVVEPRFFQRNRFSGLLIVILLLMMVEAYGLIGFVIAPPLAVALQIFAGHTMR
                     ILQRPQDDTLALAALEKRLDVAYATYNGQQELGGEPAPELGSLLQRLESLVEQTRDLT
                     E"
     gene            complement(3899..5032)
                     /locus_tag="PK829_01375"
     CDS             complement(3899..5032)
                     /locus_tag="PK829_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU39893.1"
                     /translation="MVEQSKPFIYLLVGLASIFIIISGIQNLAFILNPILLSLVITIA
                     LIPVPGWFARRGVPGWLSLVLTFVIVLGFIGLVLLLVFFGLARLTDALPSLQAGVATN
                     GQELIDMPSTVFGYRLEEIREAATAFLTSERLTGLATRSLGILFTTVSQAFLVMLIFA
                     FMLSAALSLPSSARLGLTAESPLVQQAAALTADVRRYVTLTTGLNLAAAVGNTILLLL
                     LGVGPAVLWGLLSWFMGYIPAVGFWVAMLPPLALAFVEHGFTGALVVFIGYTLINGTV
                     ENLIKPRRMGRGLKISPVVVVISLFIWGWLLGIVGAILSTPLTLLILSVLELFEGTRW
                     IALLLRTTGTGEERAVQEHEEALGRVRGLWDRGVAAVRSATRL"
     gene            5136..5882
                     /locus_tag="PK829_01380"
     CDS             5136..5882
                     /locus_tag="PK829_01380"
                     /inference="COORDINATES: protein motif:HMM:NF039337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOU39894.1"
                     /translation="MAETTRIPYLAEMLAALSQPGCAFCRLLDATAGRLVDAILFESV
                     NDVGVREELNAARGFCRRHAALLVRTGGALGTATMMQGVIKVLLRELDGSGTEVGPPG
                     RLRALLRAGGVGGPDPAAARLADALAPRAPCPVCAHEATFTEHYIDTLLKQATPGSPL
                     AGAYAASDGLCLLHFRAVVARGQSGPALMTLVAAQRAHWQRLDGELEEFLRKSDYRFQ
                     HEKFGAERDAWQRALSAVSGQLPGHLTEGA"
     gene            5879..6316
                     /locus_tag="PK829_01385"
     CDS             5879..6316
                     /locus_tag="PK829_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU39895.1"
                     /translation="MSALTFVDADEAMRERIGRALGPVAAAHMHLGNGFTLVALDADE
                     PVGLLAVVWRALPPPLPDTVEGFIDIIEVRPAWRRRGVARQLVALAAARAGAAGAYQL
                     RAWSSADKMEAIPMWRALGFGLCPATTYPRGESVRGYFVARPL"
     gene            6427..8616
                     /locus_tag="PK829_01390"
     CDS             6427..8616
                     /locus_tag="PK829_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOU39896.1"
                     /translation="MATDTDKATPTTLTARLLSTLLAGLLIGALEVALATSYATLIFN
                     GELLPHLSRGVGMSLFSATVGITVAGLLSAIPVLICSNQDVPGAVVAVAVAAAAGMLA
                     APDVEFATAAVLIALTTFMVGATFLLLGGFRLTALVRFLPYPVAGGFLAGTGWLLVSG
                     GIHMMFGGAVSPSTLGDLLAPGALGHWLPGVVLGGLIYFLGQRVRHTLFLPGMIAGAF
                     VLFYLVAFLRGATLTTLSAGGWLLGPFPGDRLWQPISAAELGRVQWAVVWPQLPNLLA
                     GVAISAIALLLNVTGLELTLRQDLDLNQEMRAAGWANLANVLGTGFPTYHSISVTVLN
                     SRLGTASRLSGLTAAVVTGLTLLFGGAALGAFPKLIFGGLLVYLGLSFLWEWAVAARK
                     RLPPIDYAIVLIILVTIAAVGFLPGVVVGVVAAVVMFVVSYSRASVVKHELDGTSFRS
                     RVTRSPGARALLLATGTQAYYLQLQGFIFFGTAYSLLQQARARARRVDTQYVVLDFRQ
                     VIGLDSTALLSFDKMRQLARDGGFVLVVAGLSPALREQFATGGLNEEAGVLRFAPDLD
                     RAAEWIEDQVCLAAEMAGQGTLDDYWQAIVPGEATTRLIGYLARREIPAGAYLIHQGD
                     APDLIYFIESGQVTAQLEREGLVPVRLETMRGGRTVGELGFYLGTQRSAAVVAEQPTV
                     VYSLTQTELARIERDDPAAASAFHRLVVHLLGERVLHLMRAVEALQR"
     gene            complement(8665..9342)
                     /gene="nth"
                     /locus_tag="PK829_01395"
     CDS             complement(8665..9342)
                     /gene="nth"
                     /locus_tag="PK829_01395"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU39897.1"
                     /translation="MNASTPKQPLDIDEAMRRIEVAVAPYAKAAMFELAELGYATPFE
                     QLVGCIISIRTRDEQSLPLSRRLLDVARTPAAMAQLTPDEIDRLISPSTFHERKAQQI
                     CAIAQRIVAEYGGELPCDETVLLSFAGVGVKCAHLTLAVGCGQPWISVDIHVHRVTNR
                     WGYVAAPAPEQTMVALEQVLPKEYWIDINRLLVPFGKHICTGELPRCSTCPVLAMCQQ
                     VGVDRHR"
     gene            complement(9402..9686)
                     /locus_tag="PK829_01400"
     CDS             complement(9402..9686)
                     /locus_tag="PK829_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOU39898.1"
                     /translation="MNVEFSGPIWFWRGPAPFYFVTVPADESDALHVIANVVTYGWGM
                     IPVTVHIGQTVFKTALWPKDGAYIVPLKASVRKAERLAEGDVVTIRLEAG"
     gene            9821..>11900
                     /locus_tag="PK829_01405"
     CDS             9821..>11900
                     /locus_tag="PK829_01405"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU39899.1"
                     /translation="MIATKLYLPPSRPGIVQRPRLIDQLNRHLHRKLTLISAPAGFGK
                     TTLVSEWVAVCGRPVAWLSLDEAESDIVRFLTYLIAALQTIAGHMGAMALPVLETGQP
                     LPVEAILTSLVNDIAAVPNNFILILDDYHLVDNPAVDRALGFLLEHWPPQMHLAITTR
                     EDPNLPLARLRMRDQLTELRAADLRFTPSEAAEFLNQVMGLALSAEEVAALESRTEGW
                     IAGLQLAALSMKGRQNASEFIEAFAGDHRYIVDYLVEEVLRRQPEALRVFLLQTSILD
                     RLNGPLSDAVTGQPGGTARLEALQRGNFFLIPLDDRRHWYRYHHLFADVLRAHLAAEH
                     PDQLPVLHTRASQWYEQDGSAPEAIRHALAARDFERAAALIERALPVMRQHRQEATLL
                     SWFRALPDELFRRRPMLSVHAAGVFLQNGQLEGVAARLRDAERWLGKVVGERPVYADE
                     EDFQRLPGLVAMYHAGMALIRGDVAGTMQYARRVMALARADDAFIRGAASALMGLASW
                     TEGDLETAHQMFAGGMGYLQKAGFISDVIGGSLTLSDIRITQGRLREALSNYERGLQL
                     ATQPGGPPLRGAADMLVGMSEIYREYNELDAATQYLRRSKELGELNGLPKNPYRSRVA
                     LARILAARGEFDGALNLLDEAERVYEGDFSPNFRPVAAIRARLWIMAGRFSQAHNWAR
                     EQGLSAGDDLS"
BASE COUNT         1857 a         3776 c         4018 g         2249 t
ORIGIN      
        1 tttggcgttg gctaccgcga tgaggcgcgg acgctgcgga cgctgttcgt cgtgcccgac
       61 gatagcccga tgcggccgtt cgtcgaagac tactccacgc gcctgggccg gcagattgac
      121 ccgcgcggca tcatccccga cgcggccgaa gccgaccgag aactgcgcca gcagcaggtc
      181 gatctcgtcg ttgtcgtccc gcccgatccg gcggcgcaac tcaacaacaa cgagcaggcc
      241 acgttccaga tgtatcacta tgagatcgac ccctacgagc agacttacat cgagattctg
      301 gggcgcaact acatcgagga gatcaatcgc caggtgctgc tgacagccgc tgaccagggc
      361 aaggtcgagg ccgcggcgct gcaagagcgc gtcaccgcgg cgcgcgagac ggccgccgcc
      421 gcccgcgtgg ccctggaggc gggcgacgag gccgccgcgg gcatcgccgc cggagacctg
      481 gatagccaac tgggcttgct ggccgtggcc gccggctccg ggctggccct gctggggggc
      541 gtggagaaca cgttgggggc cgagacgttg caggcgcaac tgctggcgat tcaggccgcc
      601 tcggcggcat tgagcgcaga taccaccact cctgtcgccg agcgcgcggc ctcggccgcg
      661 gccatcgaag ctgacctggc cgaggtcgat ggcgtgctga gccgctacca gggcatcgac
      721 tcgcaggtgc tggtgtcccc cttccgcagc gagacgctca gcatcaccga cgtcagcctg
      781 gggccgacag acttcttcgt gccggcggtc atcgccctgc tgttgcagca catcgccgtg
      841 acgctggccg gcctgtccat cgtccgcgag cgccaggacg gcccgttgga actgttccgc
      901 gcctcgcccg tgtcggcctt ccagacgctg atcggcaagt tcattggtta tcttattctc
      961 acagcggtca tcggggcagt cctgacggcc ttggtagtct ttggcctggg cgtgcctatg
     1021 ctgggaaatt ggggatacta tgcgctggtc gtcctggcgc tattgtgtgc gtcgctgagc
     1081 ctgggtttct tcctgtcgct attggcccac acggacagcc aggccattca gtacgcgatg
     1141 ttactcctgc tggccagcat cttcttcagt ggcttcttcc tggccctcta ccggctgtgg
     1201 gaaccggtgc gtgccgtttc ctgggcgctg ccggtgacct atggcattaa tctgttgcaa
     1261 tcagtgatgc tacgggggca aatgccgggt atgatcatgt tgggcgcgtt gttcctattc
     1321 gccctggtct tcttcgtgct cgactggtgg cgtctcagcc gtctgatggc tcgccagtag
     1381 ttgggcagac gtgtttgccg gagtatgacc tatgctggac catcgccccg ctctggagtt
     1441 acccgaactg tcggtcaggc aactggtgac ggccaccctg gtcgtcggcc tggttgccct
     1501 gtcgatctgg ctcgtctttc gcttcaatca ggtgatcctg atcctcattg ccggcatcat
     1561 catcagcact gccctgcagc cggttgtcaa ctggctgcag cggcggggtt tgccggccgg
     1621 cgcggcggtg ctgctgctgt ttggcgtgct ggcgctgatc gtgggcctgt tcctgcgctt
     1681 cggcgtgcct ctgatcgccg accaggcggc caatatcggc gcgcaactcg gtgacgtcta
     1741 cgccggcttt gtccagcggc tgggccagtc gtctaatctg ctgattcggc ggctggccga
     1801 ggtcatcccg ccccagatgg gcctgccggc caatgtcccg gccgcgccag tggagggtca
     1861 gggcgtggag gtagtcgagt caccattcgc gcaagtgtgg ctgaccctcg gaaccgtggc
     1921 cagagtcatc tataagactg cggcgatctt ctttgtggcc ttcttctgga ctgtcgagtc
     1981 cgaccgcatc aagcgcgtgg gcatcgccct gctgccgctc aaccggcgcg aacccattcg
     2041 tgacctcatc accgagatcg aagggcgcgt cggcggttac gtctccgggc aggtgctggt
     2101 cagcggcatc atcggcacgt tgtcgctgat tgcctacctc atcatcggca tcccctatgc
     2161 cctggccctg gctctggtca tcgccgtgat ggaattcatc cccgtggtcg gcccgctcat
     2221 tggcgcgatc ccgtcgatca tcattgccct atcgatcgcg ccgtcaaagg ccctctgggt
     2281 ccttcttacg tcgctgatca tccaccagct agaggccaac ttctttggcc cgcgggtgat
     2341 gaagcggacg ctgggcatgc gcccgctggt gacgctggtg gccatgaccg ccttcggcac
     2401 gctgtttggc atcctgggcg cgctcatcgc cctgccgctg acggccatca tccaaatgct
     2461 cctggagcgc ttcatgctga gcaacacgcc ccagaccgag ccaaccggcg agcgcgaccg
     2521 cattggcctg ctgcgctatc agacaatgga gcttgtggag gatgtccgca aggtgctgcg
     2581 ccagcgggag aacgcgccgc ccgacgagga ggggacgcac ctggaagatg tgatcgagac
     2641 gatcgccctc gatctggaca agctgctgag ccagtctcgc aacggcgcgg aggcatcatg
     2701 aaacgcctgg cgtactacgt tttgatcatt gccgtgacgg tagcgatgct gatcctgctg
     2761 tggcaatttc gaagtgtgtt gctcctgctg ctgttatcgc tgatgttggc cgcggtgctg
     2821 cggccgagcg ccgaatttct aaagtcgcgt gggttgccgg tttcgctgtc gtggttgctg
     2881 gtgtatctgg gcttgtttgc cgtcgtgggc ctgtccatct atctgcttgg cggtcacctt
     2941 ctggacgagc ttcagaagct gagcaactac ctcgttattc tctatgactt cacctatcgc
     3001 agttggtcgg gcggcagcgg cattcagcag gccattgtcc agcgcattcc cccgccggat
     3061 caactgccgg aagtgatggc cgggccgtcg gggctgtcga tcctgcggct gatctttggc
     3121 gtggggcaaa acgtcgtcac cgtgggcgcg tcgttgctga tcgtcatcgt gctaagtctc
     3181 tattggagca ccgaccggga gcactttgaa cggctgtggc tgtcgctgtt gccgccgggc
     3241 aagcgcgtgc cggcgcgcag catctggcaa accgccgagg ccagcatcgg caactatctg
     3301 cgcagcgagc tggtgcaggc actattggcg gtggtgctgc tggcggtggg gttccggctg
     3361 ctggggctag attacccgct gctggcggcg ctgttggcgg ggctgttctg gctcgttccg
     3421 atggccggct ttgtctttgc cgcggtggtg gcctttttgg ccgggatggc cacgaacggc
     3481 cacgtgcaga cggcgctgtt cgctttggtg ctgacggctg gcgtgctggc ctttttagag
     3541 ttcgtcgtcg agccgcgctt cttccagcgc aaccgcttca gcggtctgct gatcgtcatc
     3601 ctgctgctga tgatggtcga ggcttatggc ctgatcggct ttgtcattgc cccaccgttg
     3661 gccgtggccc tgcagatctt tgccggccat accatgcgca ttttgcagcg gccgcaagac
     3721 gacaccctgg cgctggcggc gctggagaag cggctggacg tggcctacgc aacctacaac
     3781 ggccagcagg aattaggcgg cgagccggcg ccggagttgg gcagcttgct gcaacggctg
     3841 gaaagcctgg tggagcagac gcgcgacctg acggagtagc ggcgtcgttc gccggggctt
     3901 acaggcgggt agcgctgcgc acggcagcca cgccgcggtc ccacaggccc cgcacgcggc
     3961 cgagcgcctc ttcgtgttcc tggacagcgc gctcttcgcc cgtgcccgtc gtgcgtagca
     4021 gcagggcgat ccagcgcgtg ccctcgaaca gttccagcac gctgaggatg agcagcgtca
     4081 gcggcgtcga gagaatggcc ccgacgatcc ccagcagcca gccccagatg aacagcgaga
     4141 tgacgacgac gacgggcgag atcttcagcc cccggcccat gcgccgcggc ttgatgaggt
     4201 tctcgaccgt gccgttgatg agcgtgtagc cgatgaagac gacgagcgcg ccggtgaaac
     4261 cgtgctcgac gaaggccagc gccagcggcg gcagcatggc cacccagaag cccacggccg
     4321 gaatgtagcc cataaaccag gagagtagac cccagagaac cgctgggccg acgccgagga
     4381 gcaatagcag aatcgtgttg cccacggcgg cagccaggtt aagaccggtt gtcagggtaa
     4441 cgtaacggcg cacgtcagcc gtcagcgcgg cggcctgctg caccaacgga ctttcggcgg
     4501 tcaaccccag gcgcgcgctg ctgggcagcg acagcgccgc gctgagcatg aaggcgaaaa
     4561 tgagcatgac caggaacgcc tgcgagacgg tggtgaagag gatgcccagg gaacgcgtgg
     4621 ccagtccggt cagccgttca ctggtcagga aagcggttgc cgcctcacga atctcctcca
     4681 ggcgatagcc gaagacggtg ctgggcatgt cgatgagttc ctggccattc gtggcgacac
     4741 cggcctgcaa actgggtagt gcgtcggtca gccgggccag cccgaagaag accagcagca
     4801 acaccagccc gatgaacccc aggacaatga caaaggttag caccagcgac agccagccgg
     4861 gcacgccgcg ccgggcaaac cagccgggca cggggatgag ggcgatggtg atgaccagcg
     4921 acagcaggat cgggttcagg atgaaggcaa ggttctggat gccgctgatg atgatgaaga
     4981 tgctggccag accgacgagc aagtagatga agggctttga ctgttcgacc atgggaggac
     5041 cctcgttgcg ttgcgattga gcaatgaccc aagcaggaat tctacaccat gctatcatcc
     5101 tgggcagaga attcgcccgc gctggaggaa ttggtatggc cgagacgacg cgaatcccct
     5161 atctggccga gatgctggcc gcgctgtcgc agccgggctg cgctttctgc cggctactgg
     5221 acgcgacggc cggccgtctg gtggacgcca tcctgtttga gtcggtcaac gacgtcggcg
     5281 tgcgcgagga actgaacgcc gcccgcggct tctgccgccg ccacgcggcg ctgctggtgc
     5341 gcaccggtgg ggcgctgggc acggcgacga tgatgcaggg ggtgattaag gtgctgctgc
     5401 gcgagttgga tggcagcggc acggaggtcg ggccgccggg ccgtctgcgg gcgctgctgc
     5461 gggccggggg cgtcggcggg ccagacccgg ccgcggcgcg cctggccgac gcgctggccc
     5521 cgcgtgcgcc ctgccccgtc tgcgcccatg aggcgacctt tactgagcac tacatcgaca
     5581 cgctgctcaa acaggccacg ccgggcagcc ccctggccgg ggcctacgcc gcctccgacg
     5641 gcctatgcct gctccatttc cgcgcggtgg tggctcgcgg ccagtctggc ccggcgctta
     5701 tgacgctcgt ggccgcccag cgcgcccact ggcagcggct ggacggcgag ttagaggagt
     5761 tcctgcgcaa aagcgactat cgcttccagc acgagaagtt cggcgcggag cgcgacgcct
     5821 ggcagcgggc gctgtcggcc gtctccgggc aactgccggg ccacctgacc gagggggcgt
     5881 gagcgcgctg acgttcgtag acgctgacga ggccatgcgg gagcgtattg ggcgcgcgct
     5941 ggggccggtg gctgcggccc acatgcacct gggcaatggc ttcacgctgg tcgcgctcga
     6001 cgcggatgag ccggtggggc tgctggcggt ggtctggcgg gcgctgccgc cgccgttgcc
     6061 ggatacggtc gagggcttca tcgacatcat cgaggtgcgg ccggcgtggc ggcggcgtgg
     6121 cgtcgcccgg cagttggtgg cgttggccgc ggcgcgcgct ggcgcggctg gggcctacca
     6181 gttgcgcgcg tggagttccg cggacaagat ggaggccatt cccatgtggc gggcgttagg
     6241 cttcggcctg tgcccggcga caacctaccc gcgcggcgag tctgtgcgcg gctacttcgt
     6301 cgcccggcca ttgtgatttg cgtcccagtg gcttttggcc ataatcgccc cccacatacc
     6361 accaccgcaa gggccgattc gaccgtggcc tgtgctcact ttgtagcctg cgccggagcg
     6421 agtagtatgg cgaccgacac agataaggcc acaccgacga ccctgacagc gcgcctgctg
     6481 tccacgctgc tggccggcct gctcatcggc gcgctggaag tcgccctggc cacctcctac
     6541 gccacgctga tcttcaatgg cgagttgctg cctcatctgt cgcggggcgt gggcatgagc
     6601 ctcttttcgg ccaccgtcgg catcaccgtc gccggcctgc tcagcgccat cccggtgctc
     6661 atctgctcca atcaggacgt gccgggggcg gtcgtggccg tggccgtggc tgcggcggcg
     6721 ggcatgctgg cggcaccaga cgtggagttc gccacggcgg ccgtcctgat cgccttgacg
     6781 accttcatgg tgggggcaac cttcttgttg ctgggcggct tccgcctgac cgcgctggtg
     6841 cgcttcctgc cctatccggt cgccggcggg ttcctggcag gcacaggctg gctgctcgtc
     6901 agcggcggca tccatatgat gttcggcggc gccgtttcgc catcaaccct gggcgacctg
     6961 ctcgcgccgg gtgcgctcgg ccactggttg ccgggcgtcg tcctcggcgg cctgatctac
     7021 tttctgggcc agcgcgtccg ccacacgctg ttcctaccgg gaatgatcgc cggcgccttc
     7081 gtgctgttct atctggtcgc cttcctgcgc ggcgcgacgc tgacgacgct tagcgccggc
     7141 ggctggctac tggggccgtt tccgggggac agattgtggc agcccatcag cgcggccgag
     7201 ctgggccgtg tacagtgggc cgtcgtctgg ccccaattgc ccaacctgct ggccggcgtg
     7261 gccatcagcg ccatcgccct gctgctcaat gtcaccggcc tggagttgac cctgcgccag
     7321 gatctggact tgaatcagga gatgcgcgcc gctggctggg cgaacctggc caacgttctg
     7381 ggcacgggtt tccccaccta ccattccatc agtgtcactg tgttgaacag ccggttgggc
     7441 acggccagcc gcctgagtgg gctgacggcc gccgtcgtca ccggcctgac gttactattc
     7501 ggcggcgcgg cgctgggggc cttccccaaa ctcatcttcg gcgggctgct cgtctatctg
     7561 ggcctgtcgt ttctgtggga gtgggctgtc gccgcgcgta agcgtctgcc gccgattgat
     7621 tacgccattg tcctgatcat cctggttacc atcgccgcgg tcggctttct gccgggcgtc
     7681 gttgtcggcg tcgttgccgc ggtggtgatg ttcgtcgtca gctatagccg tgccagcgtg
     7741 gtcaagcatg agctggacgg caccagcttc aggagccgcg tcacgcgcag ccccggcgcg
     7801 cgcgcgctgc tgttggccac cggcacgcag gcttactact tgcagctaca gggcttcatt
     7861 ttctttggca cagcctacag cctgctgcaa caggcgcgcg cgcgggcgcg gcgggtcgat
     7921 acgcagtacg tcgtcctcga ctttcgccag gtgatcggcc tcgactcgac ggcgctgctg
     7981 agcttcgaca agatgcggca gttggcccgc gacggcgggt ttgtgctggt ggtggccggg
     8041 ctgtcgccgg cgctgcgcga gcagttcgcg acgggcggcc taaacgaaga ggccggcgtg
     8101 ttacgctttg cgcccgatct tgaccgggcg gccgagtgga tcgaggacca ggtctgtctg
     8161 gcggcggaaa tggccggcca ggggacgctg gacgactatt ggcaggccat cgtgcccggc
     8221 gaggcgacga cgcggctgat cggctatttg gcgcggcgcg agattcccgc cggcgcgtat
     8281 ctcatccacc agggagacgc gcccgacctg atctacttca tcgagtccgg ccaggtgacg
     8341 gcgcagttgg agcgggaggg tctggtgccg gtacggttgg agacgatgcg cggcgggcga
     8401 acagtggggg agttaggctt ctatctgggc acgcagcgca gcgccgccgt ggtcgccgag
     8461 cagcctacgg tggtctactc gctgacgcag acggagttgg cccgcatcga gcgtgacgac
     8521 ccggccgcgg ccagcgcctt ccatcgcctg gtcgtccatc tactggggga gcgagtgctg
     8581 catctgatgc gggcggtaga ggcgttgcag cgctagcggc ccgctgtggc cgtgtcgctg
     8641 ccgggcgtgg agccgcgtca tgcctcaccg gtggcgatcc acccccacct gttggcacat
     8701 ggccagcacc ggacaggtgg aacagcgggg caattcgccg gtgcagatgt gcttgccaaa
     8761 tggtaccagc agccggttga tgtcgatcca gtactccttc ggcaacacct gttccagagc
     8821 gaccatcgtc tgttccggcg ccggcgcggc cacgtagccc caacgattgg tgacccgatg
     8881 gacgtggata tcgacactga tccacggctg cccgcagccc acggccagcg tcagatgggc
     8941 gcacttgacc cccacgccgg caaacgacag caacaccgtt tcgtcgcaag gtagctcgcc
     9001 gccgtactca gcgacgatgc gctgggcgat ggcgcaaatc tgctgggctt tgcgctcgtg
     9061 gaaggtgctg ggggagatga ggcggtcgat ctcgtccggg gtcagttggg ccattgccgc
     9121 cggcgtgcgg gccacgtcca gcaggcggcg ggagagcggc agcgactgct cgtcgcgggt
     9181 gcggattgag atgatgcaac cgacgagttg ctcaaacggc gtggcgtagc ccaactcggc
     9241 cagttcaaac atggccgcct tggcgtaggg ggccacggcg acctcgatcc ggcgcatggc
     9301 ctcgtcgatg tctagcggtt gtttgggggt ggaggcgttc atggccggaa gagtagcgca
     9361 aagcccgtag acggccaacg cgggccgggg cagcagggcc gctaaccggc ctccaaccgg
     9421 atggtgacga catccccttc cgcaagccgc tcggccttgc ggacgctggc cttgaggggc
     9481 acgatgtagg cgccatcttt gggccatagc gcggtcttga agacggtctg gccgatgtgg
     9541 acagtgaccg ggatcatccc ccaaccataa gtgacgacgt tggcgatgac gtgtagcgcg
     9601 tcgctctcat cggccggaac ggtgacaaag tagaacgggg ccgggccgcg ccagaaccag
     9661 attgggccgc taaattcgac gttcatcagc aacctttgcc gtgggaggtg gctttcgcat
     9721 cgccggggag tttagcgcgc gcggctgtat ccggccacac ccatctagcc cccttttcat
     9781 cggcgcatgt gtctcctata atgcgttcgt gccgacgcca atgatcgcca cgaaacttta
     9841 tcttcccccc tcccggcctg gaattgtcca acgcccgcgc ctgatcgacc agctgaacag
     9901 gcatctgcac cgcaaactga ccctcatctc ggccccggcc ggctttggca agacgacact
     9961 ggtcagcgaa tgggtcgccg tctgcgggcg gccggtcgcc tggctatcgc tggacgaagc
    10021 ggagagcgac atcgttcgct ttctgacgta tctcatcgcc gcgctgcaaa cgatcgcggg
    10081 gcatatgggc gcgatggcgc tgcccgtgct cgagaccggg caaccattgc ccgtcgaagc
    10141 gattttgacg agtctggtca acgacatcgc cgccgtcccg aacaacttca tcctcatcct
    10201 ggacgactat catctggtgg acaacccggc tgttgacagg gcgttaggct ttctgctgga
    10261 acactggcca cctcaaatgc acctggccat taccacgcgg gaggacccta atctgccgtt
    10321 ggcccgtctg cggatgcgcg accaattaac cgaattacgc gccgcggact tgcgcttcac
    10381 gccatcagag gcggccgaat tcctcaacca ggtgatgggc cttgcgctct cggccgagga
    10441 ggtcgccgcg ctggaaagcc gcacggaggg ctggattgcc ggcctacagc tggccgcgct
    10501 ttcgatgaag ggccgccaga acgccagcga attcattgag gccttcgccg gcgaccatcg
    10561 ctacattgtc gattatctcg tcgaggaggt cttaaggaga cagccggaag cgctgcgcgt
    10621 cttcctgctc caaacctcca tccttgaccg cctcaatggc ccgcttagcg acgcggtcac
    10681 gggtcagccg ggaggtacag cccggttgga agccctccag cgtggcaact tcttcctcat
    10741 tccgctggat gaccggcgac attggtatcg ctatcaccat ctgtttgccg atgttctgcg
    10801 cgcccatttg gccgcggagc accccgatca actgcctgtc ctgcacacgc gcgcgagtca
    10861 gtggtatgag caggacggct cagcgccgga ggcgatccgg cacgcgctgg cggcgcggga
    10921 ttttgagcgc gcggccgcgc tgatcgagcg ggcgttgccg gtcatgcgcc agcacagaca
    10981 ggaagccaca ttgctcagtt ggttccgagc tttgcccgac gaactgttcc ggcgccggcc
    11041 catgctcagc gttcatgccg ccggcgtgtt cctacagaac ggtcaactcg aaggcgttgc
    11101 ggcccgcttg cgcgacgccg agcggtggct gggtaaggtt gttggtgagc gcccggtgta
    11161 cgccgatgaa gaggattttc aacgcctgcc cgggctggtc gccatgtacc atgccggcat
    11221 ggccctgatt cgaggcgatg tcgccggcac gatgcagtat gcccggcggg tgatggcact
    11281 ggcgcgggca gacgacgctt tcattcgcgg cgcagcttcc gcgctgatgg ggctggcttc
    11341 ctggacggag ggcgacctgg agacggcaca tcagatgttt gccggtggaa tgggctatct
    11401 gcagaaggca ggctttatct ccgacgtgat cggcggctcg ctcacgctgt cggatatccg
    11461 cattacgcaa ggtcgcctgc gcgaggcgct gagcaattac gagcgcggtc tgcaactggc
    11521 cacacagccc ggtggtccgc ccctgcgcgg cgcggcggat atgctcgtgg gcatgagcga
    11581 gatctaccgc gaatacaacg aattggatgc cgccacgcaa tatcttcgaa gaagcaagga
    11641 gttgggcgaa ctgaatggcc taccgaagaa cccctatcgt tcccgcgtgg ccctggctcg
    11701 aatactggcg gcgcgaggag agttcgacgg cgcgctcaac ttgctcgacg aggccgagcg
    11761 cgtgtatgaa ggtgatttct cgcccaattt ccgtcccgtc gcggcgatca gggcgcggct
    11821 gtggattatg gccggaagat tcagccaggc acacaactgg gcacgcgagc agggcctatc
    11881 cgccggggat gatctcagct
//
LOCUS       DAOSMQ010000021        11857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5355_length_11857_cov_4.128283, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000021 DAOSMQ010000000
VERSION     DAOSMQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5355_length_11857_cov_4.128283"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PK829_01410"
     CDS             complement(<1..1112)
                     /locus_tag="PK829_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOU39900.1"
                     /translation="MSETKKEDAMTTSSSYVNGTEGVLIYRGYDIRDLGLNVTYEEVV
                     YLLWNDKLPNAHELELFRAALQAKRVVPEPVLRVMREFPRDAQPMAVLRTAVSAMGLI
                     DRDADNITSSGAREKALLLTAVVPSLVAAWERLRNGREPIPPRRGLGHAANFLYMLDG
                     EEPNPDAVAAFDSYLVLLADHGFNASTFSARVTTSTGSDFYSAITSAIGTLKGPAHGG
                     ATQRAMEQFFSAADRGDISAWFEESLDAGKRIMGIGHRIYKVEDPRAKILKPLAEKLA
                     NSSGQGQWYQVADTIQQLARQNDYFIERNLYANVDYYSAVVLYTIGLPVDQFTCIFAV
                     SRVAGWSSQVLEQLADNRLIRPKESYVGQRDQSFVPL"
     gene            complement(1147..2181)
                     /gene="rfbB"
                     /locus_tag="PK829_01415"
     CDS             complement(1147..2181)
                     /gene="rfbB"
                     /locus_tag="PK829_01415"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOU39901.1"
                     /translation="MRNLLVTGGAGFIGSNFVHYLLAQYPDYHVVVYDKLTYAGNLDN
                     LLPVQDNPRYGFVRGDICRRDEVEAAVRAHDIDTIVNFAAETHVDRSIMDPDAFIQTS
                     VYGTYTLLEVARRLGLERYHQISTDEVYGHVPAGRSSRETDPVEPRSPYAASKASADL
                     LVNAYHVTYGLPTTVTRGSNNIGPYQYPEKAVPLFATNAIDGLPLPVYGDGRQMREYQ
                     YVRDHCEAIDLVLQRGQIGETYNIGTGVEVENLAMIEILLDVLNRPRSLIRHVTDRPG
                     HDRRYSLDIRKITALGWEPAHTPAEAIAETALWYRDNEWWWRKIKSGDFQQYYEAQYG
                     ERLRTAAPLS"
     gene            complement(2360..3226)
                     /gene="nudC"
                     /locus_tag="PK829_01420"
     CDS             complement(2360..3226)
                     /gene="nudC"
                     /locus_tag="PK829_01420"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOU39902.1"
                     /translation="MSTAFIPGVLPPTGPAEPALWFLFRGSKLLVANNDERATLPLLA
                     VPADLALAPVRWQYLGRLEEAGQATHCFSAELDEATAPPPGMEFANLRALYPRLPETL
                     FQLAGRAVQIVDWDRNHQFCSRCGTPTRDQIGERSKMCPACGLVSYPRLSPAIIVRVE
                     RDDAAGRRILLARAQRFPTAMFSVLAGFVEPGETLEECVQREIHEEVGLRVSNIRYFG
                     SQPWPFPNSLMIAFVADYDAGEIVVDPVEIAEAGWFTADALPNTPMPPSIAHRLIASW
                     RDGAAGPARQRL"
     gene            complement(3236..4189)
                     /locus_tag="PK829_01425"
     CDS             complement(3236..4189)
                     /locus_tag="PK829_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39903.1"
                     /translation="MNTSDESLPAPAGPSGSRSLLSRSPVLAALAVFLLLAGLILVVS
                     TLLSQDRRPLYPNLPTFTPLSETLASEPAVLTFTELNADPDAYRDRRIQVSGDFASMT
                     PPTCAPFAGPVIRWTLVAEELQLNAVGFENVLRLLAEGTPMTVTGTWRLYRGPAGCGK
                     EPPDANVWYLAVDQILEPNPLLGGGGAALTVIAGSPLPTLPPLNLEAPTEEATPTLEA
                     TPAEATPQPTGLPEVSPTAGQPVPITPVGTPTLTETPTATTTVDPLATPTLTATPTTT
                     GTPGPSPTPSATTEGGELTPGIPTATLSGTGYPDDGTPAYP"
     gene            complement(4186..6078)
                     /locus_tag="PK829_01430"
     CDS             complement(4186..6078)
                     /locus_tag="PK829_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU39904.1"
                     /translation="MALLAPTALVLALLAVPILLLYMLRLRRREQPVSSTWLWRALVR
                     DRAANAPWQRLRRNLLLFLQLLILAALVLALARLALRRPGLGGNLIVLLDASASMGAS
                     DGSPTRFDDARAAVDGLIGNLSGDDRLTLIRVGQTAEVLAAATTDRILLRRALAVAAP
                     ENGPADWPAAFALAAGSTQGLSNPRIAIISDGGLPEGLPPLPAEVTFLPVGRAGENLA
                     ITAQAARATASGPQLLVAVSNSGTTARSALLSLYLDNVLYDSRRVELAAGAQSAQTWS
                     LPAGATLARTQLEPRDGLPDYLAVDNNAWTPLDAGSTRRVMLISDGNLFLERIFTVLP
                     GYELFRMSSAEATQATAAEEPFDLTIYDGVPLPDGPLPSSALIFNPQPPSGAGADGSS
                     PLVTVTGTFTETAAVRIADSPLLENVDWGSFAIANAQQVEAPTLPAVVSAAAGPLLLA
                     GESNGRRVVVFTFDLRASDLPLQIAFPIIMANITAWLNPGRVIAADNSQPGSTVTLLP
                     DGRAVRATVTLPDGSIWARDIANAAQPVVFDQTQQPGVYRLTTTDAAGAATPAGAFAV
                     NFFDAGESHIQPAASLQVGQAQVTAATGGFEGLRELWPWLLAAALAVLLVEWWVAYRR
                     PLFRTR"
     gene            complement(6080..6991)
                     /locus_tag="PK829_01435"
     CDS             complement(6080..6991)
                     /locus_tag="PK829_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU39905.1"
                     /translation="MRLFDDATLRTLERLSLWADEVRVGVMKGNRRSRRRGASIEFAD
                     YRDYTQGDDLRRLDWNVFARLERPFIKLTEEEEDLAVHVLIDTSGSMDWPAGDDSENK
                     LRYGLRLSGALGYIALSSGDLVQVTLFDGRGQSAWGPFRGRQNGWPLLQFLEASLAAR
                     AGGDAAARQTVTGPFLRGYAQRAHRPGLLFLLSDLFAPADYRDGLVALLARGYEVALL
                     HLLSPDEAAPELAGDLRLLDVETGDAAEVSLDPQALEEYAARLAAWQEELRRFCGPRG
                     IHYASVTTDQPWDALIRGALRREGVVR"
     gene            complement(6995..7996)
                     /locus_tag="PK829_01440"
     CDS             complement(6995..7996)
                     /locus_tag="PK829_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOU39906.1"
                     /translation="MNAVDAPEIDAFRDTAAAIRAELAKAIVGQEGVVRHVLVGLVSN
                     GHTLLEGVPGLGKTLLIRSLGQALDLRFSRIQFTPDLMPADITGTEIMEERDGGRRGF
                     RFQHGPIFANLVLADEINRATPKTQSALLEAMQERQVTVANTTYPLPAPFFVLATQNP
                     IEQEGVYPLPEAQLDRFLFKINVPFPSAAEVSEILNRTTGETEPVLSPVANGETLMGM
                     QRVARQVPVPHAVADYIARLIVATHPGQSASRLVNQAIRYGASPRGGQALVLGAKALA
                     LFDGRYNVSYDDIAAIAPAALRHRLLLNFEGQAEGISADDVVADLLAAVPREPNGRA"
     gene            complement(8057..9697)
                     /locus_tag="PK829_01445"
     CDS             complement(8057..9697)
                     /locus_tag="PK829_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU39907.1"
                     /translation="MAEIVLKLDHVAVSLAGRPIFTDLTWEIQRGQRIGLVGPNGAGK
                     STLMKLVAAEFGPDAGSVFRLSGLDWGRLEQEPQLPPGRTVLQEALTALPALGAIERQ
                     LDELADHMSRPEVYSQPTALSKALKAQERLLHQFEQLDGPRYESRVKETLVALGLDHS
                     RWDTLTEHLSGGQKKLIMLAKLLVQQPGLLLLDEPDNHLDLPAKRALEGIIRAYPGTV
                     VIISHDRYLLDEVATHIAELENGRLTQYLGNYTAYTTERQLRRLRQQQMYAAQQKEIA
                     RIEAAIARFELWASIVVNERHIRQARSRQKMLDRMDKVEKVTDVRRMTLELNGWRGSN
                     KVLELAGVARTFDNGRTLFRDLNLTLWHGERVGLVGPNGAGKSALFRLLLDPDSASAG
                     EVKVGPSIQIGYYAQEQETLDPAQDLITCIRAAAPLTREAAVAFLLRFLFSYDQTTQP
                     VGKLSGGERSRLQLARLVLQRPNLLLLDEPTNNLDIPAIEVLEETLDEFVGTVLVISH
                     DRYFLDRAVDRIVELRDGRLTEYVGGYTDYLAERGYDE"
     gene            10080..11441
                     /locus_tag="PK829_01450"
     CDS             10080..11441
                     /locus_tag="PK829_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU39908.1"
                     /translation="MNRISRLAAFLLPVLLLLAACDLLDNTPAAYPVATFAPTASLLT
                     LGPTPAAATSVPAQLPDASVSSLRLWLPAEIGARTPEGAQELADQITAFEASQSLEVL
                     VEQKPVEGAGGILSYLRTGTAVAPSVLPDVIALPAVMLTDPTIRQMVQPLDGLIDPAM
                     LEDAYTDAAAQVVGGPETGGSPARFYGVPFVSAGLTHLVYARASITDTIPMDWTTFIS
                     DTNHTLVLPADSRDGALFGLQFYLAEGGTLVNENGQPDLQVEPLARALRQIAGRPENL
                     LQSRQLKTLDEAWQYYQVGQSGFLWVRADYYLGRRAEAGAAEAQVSDPHLLGYSRVPG
                     PGGPLTPLTTTWVWAVATANPARQRPAADLIRALTAPQALATWAEQSQLLPARREAMT
                     LLAAEEEYYHFAGEELERARPMPISESSRQLAAIGDAVFQVLTTDQSPEAIAEATVLS
                     LRQ"
     gene            complement(11390..>11857)
                     /locus_tag="PK829_01455"
     CDS             complement(11390..>11857)
                     /locus_tag="PK829_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOU39909.1"
                     /translation="LARQLAGRLAADALLILDAGHAPENFLGPIVRFRPDAVLFLDAA
                     RGGGAPGDIVWLRPAEADGRGGSTHAVSLEMLADYLTAETGAVIRVLGIEPSLSSSRR
                     AGCQPASCRAPAEFPFSEGLSPAVAAAADEVAATLIAYCRKLSTVASAMASGD"
BASE COUNT         1938 a         3809 c         4135 g         1975 t
ORIGIN      
        1 agcgggacga agctctggtc ccgctggccc acatagctct ccttggggcg gatgaggcgg
       61 ttgtcggcca gctgctccag cacctgcgaa ctccagccgg cgacgcggct gacggcgaag
      121 atgcaagtga actggtcaac gggcaggccg atggtgtaga ggacgacggc cgagtagtaa
      181 tcgacgttgg cgtacaaatt gcgctcgatg aagtagtcgt tctggcgcgc caactgctgg
      241 atcgtgtccg cgacctggta ccactggccc tggccgctgc tgttggccag cttttcggcc
      301 agcggcttca ggattttggc ccgcgggtct tcgaccttgt agatgcgatg gccgatgccc
      361 atgatgcgct tgccggcgtc cagcgactcc tcgaaccagg cgctgatgtc gccgcggtcg
      421 gcggcgctga agaactgctc catggcccgc tgggtggctc cgccgtgggc cgggcctttc
      481 agcgtgccaa tggcgctggt gatggccgag tagaagtcgg agccggtgct ggtcgtcacg
      541 cgggcggaga aggtcgaggc attgaagccg tggtcggcca gcaagacaag ataggaatcg
      601 aacgcggcca cggcgtcggg gtttggctcc tcaccgtcga gcatgtacag gaagttggcg
      661 gcatggccca gcccgcgacg tggcggaatc ggctcgcgcc cgttgcgcag gcgctcccag
      721 gcggcgacca ggctgggaac gaccgctgtc aacagcagcg ctttctcgcg cgcgccgctg
      781 gaggtgatgt tatcggcgtc gcggtcgatc aggcccatcg cgctaacggc cgtgcgcaag
      841 acggccatcg gctgggcgtc gcgtggaaac tcgcgcatca ctcggagcac cggttccggc
      901 acgacccgct tggcctgtaa cgccgcccgg aacagttcca gctcatgggc attgggcagc
      961 ttgtcgttcc acagcagata gacgacctct tcataggtca cattcaggcc caggtcgcgg
     1021 atatcgtagc cgcggtagat caatacccct tcagttccat tgacatagct cgatgacgtc
     1081 gtcatcgcat cttctttctt ggtttcggac atcgtctgcg ctcctatgtg gctaactgga
     1141 cgtcttttat gaaagcggcg cggctgtgcg tagccgctcg ccatattgcg cttcgtagta
     1201 ctgctgaaaa tcgccgctct taattttgcg ccaccaccat tcgttgtccc gataccagag
     1261 ggccgtctcg gcaatggctt cggccggcgt gtgggccggc tcccagccca gggcggtgat
     1321 cttgcggatg tcgaggctat agcggcggtc gtggccgggc cggtcggtca cgtggcggat
     1381 aaggctgcgc ggccggttca gcacgtcgag caggatttcg atcatcgcca ggttctcgac
     1441 ctcgacgccg gtgccgatgt tgtacgtctc gccgatctgg ccgcgctgca aaaccaggtc
     1501 gatggcctcg cagtggtcgc ggacgtattg atactcgcgc atctgccggc cgtcgccata
     1561 gacgggcagc ggcaggccgt cgatggcatt ggtggcgaac agcggcacgg ccttttccgg
     1621 atactggtag gggccaatgt tgttggagcc gcgggtgacg gtggtgggca ggccataggt
     1681 gacgtggtag gcgttgacga gcaggtcggc gctggctttg ctggccgcgt aggggctgcg
     1741 cggctccacc gggtcggtct cgcggctcga ccgcccggcc ggtacgtgcc cgtagacctc
     1801 gtcggtggaa atctggtgat agcgttccag ccccaaccgc cgggcgacct ccagcagcgt
     1861 gtacgtgccg tagacgctcg tctggatgaa ggcgtcgggg tccatgatag aacggtctac
     1921 gtgggtttcg gcggcgaaat tgacaatggt gtcgatgtcg tgcgcccgga cggccgcttc
     1981 aacctcatcg cgccggcaga tgtcgccgcg gacaaagcca tagcggggat tgtcctgaac
     2041 cggtagcagg ttgtccaggt tgccggcgta ggttagcttg tcgtaaacca cgacgtgata
     2101 gtcgggatac tgcgccagca gataatggac aaagttggaa ccgatgaagc ccgctccacc
     2161 cgtaaccagt aaattcctca acaatgcctc cgcgtttgta gttacacagg ctgcccgtga
     2221 ccggcacggc cgccggtcat tgccccatct gccgcccgtt taacggcatg ctgtcccttg
     2281 cgaacatcac ggccgcctcc ggtaaggccg ggggccagat gatcctgatt gtagcccatg
     2341 ccccccaaac ggtaaacact tataggcgtt gtctggccgg gccagccgcg ccatcgcgcc
     2401 aggaggcgat cagccggtgg gcgatgctgg gcggcatagg tgtgttcggc agcgcgtcgg
     2461 cggtgaacca gcccgcctcg gcaatctcga ccggatcgac gacgatctcg ccggcgtcat
     2521 agtcggccac aaaggcgatc atgagtgaat tgggaaacgg ccacggctgg ctgccgaagt
     2581 agcgtatgtt gctgacccgc aggccgacct cttcgtggat ttcgcgctgg acacattcct
     2641 ccaacgtctc gcccggttcg acgaaaccgg ccaggacgct gaacatggcc gtggggaagc
     2701 gttgcgcccg cgccaacagg atgcgccgcc ccgcggcgtc gtctcgctcg acgcgaacaa
     2761 tgatggctgg cgaaaggcgc gggtagctga ccaggccgca ggcggggcac attttggagc
     2821 gctcgccaat ttggtcgcgg gtgggcgtgc cgcaacggct gcagaactgg tggttgcgat
     2881 cccaatcgac gatctgcacg gcccgaccgg ccagctggaa gagcgtctct ggcagccgcg
     2941 ggtaaagcgc gcgcaagttg gcgaactcca ttcccggcgg cggcgcggtc gcctcgtcca
     3001 attcagccga aaagcagtgg gtggcctggc ccgcttcctc cagacgaccc agatactgcc
     3061 agcgaacagg cgccagggcc aggtcggccg gcacggccaa caggggcagt gtcgcccgct
     3121 cgtcgttgtt ggcgaccagc agctttgacc cgcgaaagag gaaccacaac gccggttcgg
     3181 ctgggccggt tggcggcagg acgccgggga tgaacgccgt gctcatggct gttgcttaag
     3241 gataggcggg cgtgccgtca tcggggtagc cagtgcctga gagggtcgcc gtgggaatgc
     3301 ccggcgtcag ctcgccgccc tcggtcgtgg ccgaaggcgt tgggcttggc ccgggcgtgc
     3361 cggttgttgt cggcgtggcg gtcagggttg gcgtggccag agggtcaacg gttgtcgtcg
     3421 cggtgggcgt ctcggtcagc gtcggcgtgc ccacaggggt gatcggcacc ggttggcctg
     3481 ctgtcgggct gacttccggc agccccgttg gttggggcgt ggcctccgcc ggcgttgctt
     3541 ccagcgtcgg cgtcgcctcc tctgttggcg cttccagatt cagcggcggc agtgtgggca
     3601 agggcgagcc agcgatgacg gtcagcgccg cgccgcctcc gcccaacagc gggttcggct
     3661 ccaggatttg atcgacggcc agataccaga cattggcgtc gggcggctct ttgccacagc
     3721 cggccgggcc gcgatatagc cgccacgtgc cggtgaccgt catcggcgtg ccttccgcca
     3781 gcagccgcag aacgttctca aagccgacgg cattcagttg tagctcttcg gccaccagcg
     3841 tccagcggat gaccgggccg gcgaatggcg cgcaggttgg tggcgtcatc gaggcgaagt
     3901 cgcccgacac ctggatgcgc cggtcgcggt aggcgtcggg gtcagcgttc aactcggtga
     3961 aggtcaacac ggccggctcg cttgccagcg tctcgctcag cggggtgaag gtcggcaagt
     4021 tggggtacag tgggcggcga tcctggctca gcagggtgga gacgaccaga atcagtcccg
     4081 ccagcagcag aaacacagcc aacgcggcca gcaccggcga gcggctgaga agtgagcgcg
     4141 acccggaagg cccggccggc gcgggaaggg attcgtcgga cgtgttcatc gcgttctgaa
     4201 gagcggccgg cggtaggcga cccaccactc gacaagcagc accgccaggg cagcggccag
     4261 aagccagggc cacaactcgc gtagcccctc gaaaccaccg gtcgcggccg ttacctgtgc
     4321 ctgtccaact tgcagactgg ctgcgggctg gatatgcgac tcgcccgcgt cgaagaagtt
     4381 gacggcaaac gcaccggccg gcgtggctgc gccggcggcg tcggtagtgg tcagacgata
     4441 gacgcccggc tgctgtgtct ggtcgaatac caccggctgg gcagcgttcg caatgtcacg
     4501 cgcccatatg gagccatcag gcaaggttac tgtcgcgcga acggcccgcc catcgggcaa
     4561 caaggtcacc gtgctgcccg gttgggaatt gtcagcggca atgacgcgtc ccggattcag
     4621 ccacgccgta atgttagcca taatgatcgg aaatgcaatc tgcaggggca ggtcagaggc
     4681 gcgcaggtca aaggtgaaga cgacgacgcg ccgtccgttg ctctcgccgg ccagcagcag
     4741 cggccccgca gcggcactga ccaccgccgg cagcgtcggc gcttcgacct gctgggcgtt
     4801 ggcgatggca aaggagcccc aatcgacgtt ttccaacagc gggctatcgg cgatgcgcac
     4861 ggccgccgtt tcggtaaagg tgccggtgac ggtcaccagc ggtgacgagc cgtccgcccc
     4921 ggcgccggac ggcggctgcg ggttgaagat aagggcgctg ctgggcaggg ggccgtccgg
     4981 caggggcacg ccatcgtaga tggtcaggtc gaacggctcc tcggccgcgg tcgcctgtgt
     5041 cgcttctgct gatgacatgc gaaacagttc gtagccgggc aagacggtga agatgcgctc
     5101 caggaacagg ttgccgtcgc taatcagcat cacgcggcga gtgctgccgg cgtccagcgg
     5161 cgtccaggcg ttgttgtcga cggccaggta gtcgggcagg ccgtcgcgcg gctccagttg
     5221 cgttcgggcc agcgtggccc cggccggcag cgaccatgtc tgcgccgatt gcgcccccgc
     5281 ggccagttcc acgcgccgcg agtcgtagag cacgttgtcg agatagaggc tcagcagggc
     5341 cgagcgggcc gttgtgccgc tgttgctgac ggcgacaagc agttgcgggc cgctggccgt
     5401 cgcgcgggcg gcctgggcgg tgatagccag gttctctccg gcgcgcccca cgggcaggaa
     5461 ggtcacctcg gccggcagcg gtggcagacc ctctggcagg ccgccgtcag agataatggc
     5521 gatgcgcggg ttgctgaggc cctgggtgga gccggcggcc agggcaaagg cggccggcca
     5581 gtcggccggc ccgttctccg gcgcggcgac ggccagcgcg cgccgcagca ggatgcgatc
     5641 ggtcgtggct gcggccaaca cttcggccgt ctggcccacg cggatgagcg tcagccggtc
     5701 gtcgccgctc aggttgccga tgagaccgtc gacggccgcg cgggcgtcgt cgaagcgcgt
     5761 ggggctgccg tcgctggccc ccatggacgc cgaggcatcg agcaggacga tgaggttgcc
     5821 gcccaggccg ggccgccgca gcgccagccg cgccagcgcc agcaccagcg ccgccagaat
     5881 tagcagttgc aggaagagca acagattgcg ccgcaggcgc tgccagggcg cgttggcggc
     5941 gcggtcgcgc accaacgcgc gccacagcca ggtgctgctg accggctgct cgcgccgccg
     6001 caggcgcagc atgtagagca gcaggatggg cacggccagc agagccagga ccagggccgt
     6061 gggagccagc agagccatgt taacggacga ccccctcgcg gcgtaaggcc ccgcggatga
     6121 gcgcgtccca gggctggtcg gtggtcacac tggcgtagtg gatgccgcgc gggccacaga
     6181 agcggcgcaa ctcctcctgc cacgccgcca gccgggcggc gtactcctcc agtgcctgcg
     6241 ggtcgaggct aacctcggcc gcgtcgcccg tctccacgtc gagcaggcgc aggtcgccgg
     6301 ccagttcggg cgcggcctcg tcgggactga ggaggtggag cagggcgacc tcgtagccgc
     6361 gggccagcaa ggcgaccagg ccgtcgcggt agtcggccgg ggcgaagagg tcgctgagca
     6421 ggaagagcag gccggggcgg tgggcgcgtt gcgcatagcc acgcaggaaa gggccggtga
     6481 cggtctggcg ggcagcggca tcgcccccgg cacgggcggc caggctggcc tccaggaatt
     6541 gtagcagcgg ccaaccgttc tgccgcccgc ggaacggccc ccaggcgctc tggccgcggc
     6601 cgtcgaacaa cgtcacttgc accagatcgc cggaggacaa ggcgatgtag cccagcgcgc
     6661 cagacagccg caggccgtag cgcagcttgt tttcactgtc gtccccggcc ggccagtcca
     6721 tgctgccgct ggtgtcgatc agcacatgga cggccaggtc ttcttcttct tcggtcagct
     6781 tgatgaacgg ccgctccagc cgggcgaaga cgttccagtc gagccggcgc aggtcatccc
     6841 cctgggtgta atcgcggtaa tcggcgaact caatcgacgc gccacggcgg cggctgcggc
     6901 ggttgccctt catgacaccg acgcgcacct cgtcggccca caggctcagg cgctccagcg
     6961 tgcgcagggt ggcgtcatcg aagaggcgca ttggctaggc gcggccgttc ggctcgcgcg
     7021 gcacggcggc caacaggtcg gcaaccacgt cgtcggcgct gatgccttcg gcctggccct
     7081 cgaagttgag cagcagccgg tggcgcagcg ccgccggggc gatagcggcg atgtcgtcgt
     7141 agctaacgtt gtagcgaccg tcgaacaacg ccagcgcttt ggcacccagc accagcgcct
     7201 ggccgccgcg tgggctggct ccgtagcgga tcgcctggtt caccagccgg ctggcgctct
     7261 ggccagggtg cgtggccacg atcagccggg cgatatagtc ggcaacagcg tggggcacgg
     7321 gcacttgccg ggcgacgcgc tgcatgccca tcagcgtctc gccgttggcc accggggaga
     7381 ggactggttc cgtctcgccc gtagtgcggt tgagaatctc gctgacctcg gccgccgatg
     7441 ggaagggtac gttgatcttg aacaggaagc ggtcgagttg ggcttccggc agcgggtaga
     7501 caccctcctg ctcgatgggg ttctgcgtgg ccagcacgaa aaagggcgcc ggcagcgggt
     7561 aggtcgtgtt ggccacggtt acctggcgct cctgcatggc ctccagtagg gctgactgcg
     7621 tcttgggcgt ggcccggttg atctcgtcgg ccagcaccag attggcgaag atcggcccat
     7681 gctggaagcg gaagccacgc cggccgccgt cgcgctcttc catgatctcc gtaccggtga
     7741 tgtcggccgg catcaggtcg ggcgtgaact ggatgcggct gaagcgcaga tcgagcgcct
     7801 ggcccaatga gcggatgagc agcgtcttgc ccagccccgg tacgccctcc agcagcgtgt
     7861 ggccgttgct gaccaggccc accaacacgt ggcgcacgac cccttcctgg ccaacaatgg
     7921 ccttggccag ttcggcgcga atggccgcgg cggtgtcgcg gaaggcatcg atctccggcg
     7981 cgtcaacggc gttcatagtg cccatagctc tgttcatcgg cgggattata cacgtcttgg
     8041 cccgaccgcg cgcggatcat tcatcgtagc cgcgctcggc cagatagtcg gtatagccgc
     8101 cgacgtactc cgtcagccgc ccatcgcgca gttcaacgat gcgatcgact gcccggtcga
     8161 ggaaatagcg atcgtgggag atgaccagca ccgtgccgac gaactcgtct agcgtctcct
     8221 ccagcacctc gatggccggg atatcgaggt tgttggttgg ctcgtcgagg agcagcaggt
     8281 ttggccgctg caaaaccagc cgcgccagtt gcagccggct gcgctcgccg ccgctcagct
     8341 tgccgacggg ctgcgtcgtc tggtcatagc tgaacaggaa gcgcagcagg aaggccacgg
     8401 ccgcctcgcg cgtcagcggc gcggcggcgc gaatgcaggt gatgaggtcc tgcgccgggt
     8461 cgagcgtctc ctgctcctgg gcgtagtagc cgatctggat gctggggccg accttgactt
     8521 cgccggccga ggcgctgtcg gggtcgagca gcagccggaa cagggccgac ttgcccgcgc
     8581 catttggccc caccaggccg acgcgctcgc catgccagag ggtcaggttg aggtcgcgga
     8641 aaagcgtgcg gccgttgtcg aacgtccggg ccacgccggc cagttccagg accttgttgc
     8701 tgccccgcca gccgttcagt tccagcgtca tccgccggac gtcggtgacc ttctcaacct
     8761 tgtccatgcg gtcgagcatc ttctggcggc tgcgggcctg gcggatgtgg cgctcgttga
     8821 cgacgatgga ggcccacaac tcgaagcggg cgatggctgc ctcgatgcgg gcgatctcct
     8881 tctgctgggc ggcatacatt tgctgctggc gcaggcggcg cagttgtcgt tcggtcgtgt
     8941 aggcggtgta gttgccgagg tattgggtca ggcggccgtt ttctagctca gcgatgtggg
     9001 tcgccacctc gtcgagcagg tagcggtcgt gggagatgat gaccaccgtg ccggggtagg
     9061 cgcggatgat gccctccaac gcccgcttgg ccggcaggtc aagatggttg tccggttcgt
     9121 cgagcagcag caggccgggt tgttgcacca gcagtttggc cagcatgatc agcttcttct
     9181 ggccgccgct gaggtgttcg gtcagggtgt cccagcggct gtgatctagc cccagagcaa
     9241 ccagcgtctc cttgacgcga ctctcgtagc gcgggccgtc gagttgctcg aactggtgca
     9301 acagacgctc ctgggccttt agcgccttgc tcagggctgt gggctggctg tagacctccg
     9361 gccggctcat gtggtcggcc agctcgtcca attggcgctc gatcgcgccc agcgccggta
     9421 gagcggtcag ggcttcttgc agcacggtgc ggccgggcgg cagctgtggc tcttgctcca
     9481 gccgccccca gtccagtccg gagagacgga agacgctgcc cgcgtccggg ccaaactcgg
     9541 ccgcgacaag cttcatcagc gtcgacttgc ccgcgccgtt ggggccaacc aggccaatgc
     9601 gttggccgcg ctggatctcc caggtcaggt cggtgaagat ggggcggccg gccaggctga
     9661 ccgcaacgtg atcgagcttc aagacaatct cggccatttt gaacttgctc cagaaaacaa
     9721 aaagccgggg tttgcgcccc ggctcggtct gacaatctcg catcaacttg acataagaat
     9781 cagtcaccag gcgcaatacg acaaggtgac cccgtgatgg aaaccaggcg gcgcgacggc
     9841 cccgattgat tcgtatagcc agtgacgatg agcatgagcg cgagcttatc tcaagagatg
     9901 ccaacaggca agttccggca accagggctt tgccaacgtc acccccggcg caccgacctg
     9961 acagatgggt ggccacgaat ggtacttcca tcagcgaacg actggccgcg cacctgtcag
    10021 gtccatggtt gcagccctgt tccggcaacg gtgctatact gccccgctgg aggcacatca
    10081 tgaaccgcat cagccgcctg gctgcattcc tgctaccggt acttctcctg ctcgccgctt
    10141 gtgatctact ggacaacacg cccgccgcct atccggtggc gaccttcgcg ccgacagcgt
    10201 cgttgctaac gctggggccg acgccggccg cggccaccag cgtcccggcg cagctgcccg
    10261 acgccagtgt tagctcgttg cgtctctggc tgccggccga gatcggcgcg cgcacgccgg
    10321 aaggagcgca ggaattggct gaccagatta cggccttcga ggccagccag tcgctggaag
    10381 tcctggtcga gcaaaagccg gtcgaagggg caggcggcat cctcagctat ctgcgcacgg
    10441 gcacggccgt cgctccatct gtcttgcccg acgtcatcgc cctgcccgcc gtcatgctga
    10501 ccgatccgac cattcgccag atggtgcagc cgttggacgg actgatcgac ccggctatgc
    10561 tcgaagatgc ctacaccgac gcggcggcgc aggtggtggg cgggccagag acgggcggct
    10621 ctccggcccg tttctacggc gtgcctttcg tcagcgcggg actgacccac ctggtctacg
    10681 cccgcgccag catcaccgac accatcccca tggactggac gaccttcatc agcgacacca
    10741 accacaccct ggtgctgccg gccgacagcc gcgatggggc gctgtttggc ttacagttct
    10801 atctggctga ggggggcacg ctcgtcaacg agaacggcca gcccgacttg caggtcgagc
    10861 cattggcgcg ggcgctgcgc cagattgccg gccgcccgga gaacctgctg caaagccgcc
    10921 agctaaagac gctcgacgag gcctggcagt actatcaggt cgggcaaagc ggctttctgt
    10981 gggtgcgcgc cgactactac ctgggccgtc gcgccgaggc aggtgcggcc gaagcgcagg
    11041 tctcagaccc acacctgctg ggctattcgc gcgtgcccgg ccccggcggc ccgctgacgc
    11101 cgctgacgac aacgtgggtc tgggctgtgg ccacggccaa ccccgcgcga cagcggccag
    11161 cggccgacct catccgcgcg ctgaccgccc cgcaagcgct ggccacctgg gccgagcaga
    11221 gtcagttgtt gccggcgcgg cgcgaggcca tgacgctctt ggccgccgaa gaagagtact
    11281 accattttgc cggggaggag ctggaacgag ccaggccaat gcccatcagc gaatcgagcc
    11341 gccagttggc ggccatcggc gacgcggtct ttcaggtatt gacgacagat cagtcgcccg
    11401 aagccatcgc cgaggcaacg gtgcttagct tgcggcagta ggcgatcagc gtcgccgcca
    11461 cctcatcggc agcggcggcc acggccggcg acagtccttc gctgaagggg aactcggccg
    11521 gcgctcggca ggacgcaggt tgacaaccag cgcgacgtga ggaggaaagg gagggttcga
    11581 tgcccagtac gcggatgacc gcgccggttt cggcggtcaa atagtcggcc agcatttcca
    11641 gcgacacagc gtgggtgctg ccaccacggc cgtcggcctc ggccggccgt agccagacga
    11701 tgtcgcccgg cgcgccgccc ccccgcgcgg catcgaggaa gaggaccgcg tcaggccgga
    11761 agcggacgat agggccgagg aagttctccg gagcgtggcc ggcgtcgagg atgagcaggg
    11821 catcggcggc cagccggccg gccaactgcc gggccag
//
LOCUS       DAOSMQ010000022        11852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5357_length_11852_cov_4.991184, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000022 DAOSMQ010000000
VERSION     DAOSMQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5357_length_11852_cov_4.991184"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK829_01460"
     CDS             <1..305
                     /locus_tag="PK829_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOU39910.1"
                     /translation="LPAEAERFIGEVLWQLGHTADAALALPAESLDSLATATSITTRE
                     QEVLRLLSEGLTNRQMADRLCVSVGTIKTHLTNIYGKLGVNCRVQAVAEAQALRVL"
     gene            complement(302..1708)
                     /locus_tag="PK829_01465"
     CDS             complement(302..1708)
                     /locus_tag="PK829_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOU39911.1"
                     /translation="MRRPFTLLPIAIILLLGAACRAPAEVAPASTPARNAIQENRATA
                     TAAPATPPPNAIQENRATATPLAATATPIYAGPLSPPCGLSLPALPVDGPAASSINLS
                     DEAALRAIVPAAAWPAIEQLRDAPASVGLVAYQVGREAEGIYWNADTPMPLASVAKII
                     TLVAYVEAVAAGELDPLEIVPLAELERFYLPNFDLGAHRRALAELRAEGRLLSPDDNP
                     AVPLSEVPWMMIRHSSNAAADYLHLRLGQARIEDTARVLGLDNPAGSHSAPCTFLGQF
                     LLMANHSRTLANDTAALAAYVDDEAAYGRDVALLADAYAGQAEFRAAEAAWRERNRRP
                     SIDTQRIFAARLAPRGTTRAYAALMALLAGNGLSNADSSFQARRYLEWPNQFENNLEW
                     GSNVGYKNGALPGVLTTVYYAYRWDDPAAVVVALFYRDLPQDTYRRWRNDLPHDELAR
                     WLLADPAAIPALAGAIAP"
     gene            complement(1720..4767)
                     /locus_tag="PK829_01470"
     CDS             complement(1720..4767)
                     /locus_tag="PK829_01470"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU39912.1"
                     /translation="MFSPSQTQSFLIAAAILLVVVVAGLLIARSLRRRRSRQALVERV
                     AELEALSSAGRAIVAAELDVDALCRFIAEQAGQVIDNRTFQVGLFEDSYYDIRYWTID
                     GQPQPVPQCFDLGKDEGGRMRDEGGLVGWVRDSQQPLLVRDFQREMDKLPARPAYHSD
                     SPPRAALFLPLVSGGRTLGIVSAQSTTPDHFSEQDMRRLTILANQAAAAIANARLFAQ
                     ERTRAAHLELVTQIARQVNAADDLDDVLEQAVSLTRQTFGFHPVTVFGLDPLTRDAII
                     QASTIPELDAANGGGPQLRLPIGRGIIGTAAATRHTVVVNNAGEDERFLAHLDGVPPE
                     LNPDTQAEIAIPLLVHNELLGVFDVQSPQVGAFGANERVVLETLAAELSSAIYKTQQL
                     SREQERAWITTAQLQVAEAIGRHDEPEEMLTAVARLAPLLAGVKLCAFLLWDEDSESY
                     TGATLVDRRGNEDAAFANVRLAIGDWNALDAVHVGREPLTTTHLPPWATQPWATQPLL
                     EGQVSPARACLRLYPLVSGPVKTLGAMLVDFDDGADDGPTPAQSASGAAPFHARREEL
                     VQNLARQTAAGLESTYLRMAQQEEAWVNTALLQVAEAVNSLTDLNEILDTIVRLVPLL
                     VGVDTVFILVWDEDRQIFQAGPSYGVSTMGRGLVETLEIDREELLAMSPGLTHDSSQP
                     RLPGAGYYAMRVPGWLETVLGTATAYNFPLVARGRLVGAKIVGLQRDRLGRTPFSNRR
                     INILNGIAQQAATAVVNNQLYRESADRARMQQELDVAHTIQASFLPDGSPSIPGCSVA
                     TYWQAARQVGGDFYDFLPLGDDKWGIAIADVADKGVPAALFMALTRTILRAVAFSHTE
                     PAQVLVRANEIIGREARSDLFVTVFYGVWDPATEHFTYANAGHNPPLLLQPNGNFQPL
                     LGHGVALGILPEISMKSHTITLRPGETIILYTDGITEALNEDFDEFGLERLQVAARAA
                     ARRSSADVVRQITDNIRDHTGQTAQFDDMTLIVLKRLGGKAVR"
     gene            complement(4839..5195)
                     /locus_tag="PK829_01475"
     CDS             complement(4839..5195)
                     /locus_tag="PK829_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU39913.1"
                     /translation="MSVWQEKLPQSNTWLIGISGRLDQSLTPKLEETLNALLDNGHVA
                     LVVDLTHATYINSGGLRCLVSAWRKAKAGDGTLTLCGLNNRLQEIFAMVGFDKVFTIL
                     DDCATARGQARQGVRS"
     gene            complement(5192..5650)
                     /locus_tag="PK829_01480"
     CDS             complement(5192..5650)
                     /locus_tag="PK829_01480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU39914.1"
                     /translation="MAREQVLVVPGRYKEIKRICEFIAAGAADAGFDANTIFHLELCC
                     DEASTNIIEHAYGDEGVGDIAISYAVEDDGFTIVLRDTGQAFDPGDVPPPPAISGTAM
                     PADELADQMRIGGLGLHFIRNLMDEVTYTADRRHGNRLVMVKKLHKDGDP"
     gene            complement(5746..6090)
                     /locus_tag="PK829_01485"
     CDS             complement(5746..6090)
                     /locus_tag="PK829_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU39915.1"
                     /translation="MAESLVDIQVEDLKRVELVRVTGRIDSSNAAEFDRVLKEVGSRK
                     QNVVLELSGVEYISSAGLRAIVALWRECQKHRGDVLLANPSERVVEVLTLAGLNTVFK
                     TFDSETAAVGSF"
     gene            6243..7424
                     /gene="queG"
                     /locus_tag="PK829_01490"
     CDS             6243..7424
                     /gene="queG"
                     /locus_tag="PK829_01490"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOU39916.1"
                     /translation="MQPPTAMLTAELKAQAAAMGFNRVGVIPAAPARRLDAYLAWIDA
                     GLHGEMGYMARPDRVARRRDPAVILPGVRSLVVVGLDYAAPPLPAEIAARLADDPSRG
                     RFSNYAWGADYHDVMTPRLEALGDWLRGQTAGEVASRAYVDTGALLERDHGETAGLGF
                     TGKNTLLIDPRAGSWFFLGVLLTTLPLDYDQPLVASPSCGRCRRCLDACPTAAFPAPY
                     VLDARRCISYLTIELKGWIPAELRPLMGNWVYGCDVCQAVCPFNRFAAPAAEAAFRPA
                     SWDAVAPPLLDLLALDDEGFRRRFAHGPVGRIGRARLVRNACVAAGNWGSAAAVAALA
                     ALLTDAAPIVRGHAAWALGRIGGQAAGAALAAALPAEADPDVRHALSAATQPRRETRG
                     Y"
     gene            complement(7381..8370)
                     /locus_tag="PK829_01495"
     CDS             complement(7381..8370)
                     /locus_tag="PK829_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU39917.1"
                     /translation="MTNWTKRKRLEATISGEPTDRPPVALWRHWPGDDQHAAALATAH
                     LKWQADYDWDLLKVGPASSYSVVDWGVEDRWDGHIEGTRTTTRYPVTQPTDWEALRPT
                     DPSRGMLATQIEALRLIGQTLRGEVPFMATIFSPLSMAKHLAGNEVLLSHMRAHPDAL
                     HRGLATLADSIITFIDAARATGIAGIYYAVQHAVYPLLSRDEFAAFGRPYDRRVLDSA
                     ADLWANMVHLHGTRVMFDLVADYPAPLLNWHDRETGISLRDGLAGFPGAASGGVSQWT
                     IHRDQSDDLLAEAAEARAQTGDRRLVLGTGCVIMTTTPLFNIRAFRGAAESPR"
     gene            complement(8388..10766)
                     /locus_tag="PK829_01500"
     CDS             complement(8388..10766)
                     /locus_tag="PK829_01500"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU39918.1"
                     /translation="MSHRRLRPTNPAALLPLALLLLPLAYVLTLARSVVFGDPTEYTF
                     VAHILGIAHPPGYAFYTLLGKLFQTLVPLGEVSWRMHLLSAAVATGTALFIYGTVRAA
                     ARMLLPALHAPWDRVAAVFAALSYGLAADVWQHAIHANPHIVTAAFLAANLFFLTRWA
                     AGGTARPRWLYAFALSAGLGVTHHPLTVMSFPAYAVFILLARPRIWREWRVWLGMAGC
                     AVLGLAVWLYFPIRSATAPPFGPATMTTLSGFLDHVLARGLSESLPFFGLADAPARAL
                     VFWSLLRLQFSLPVIFLAMLGVVWPLLARRRGGAAVPAWKLTTLYVLALAGNYAFVMS
                     LRAQDIMAYLLGLLVILAVLAGLGLYGLLALTATRLRPAPALLALLAAALFLLGPGLQ
                     VARNAPAISLRDETAAADYANAVFDRFAGTAEGATLLNDWEHMTPLWYARFVDERWPD
                     EADVRPILVSTARPWLESVFDTLPGGPVYLSNYRREIVAAGFRLRPDGPFYRVVEPGD
                     ASLPSDLTPLAGEGAAQLAGYALPQRSVTAGDYVPLTLALRAPAGTADYYAPVVRVGA
                     LSFPFTTDSHLISPQWQPGEVIVERFDFALPHDLPGGVYPVTVALRNLSTDETGPELP
                     LGELIVAVPRQQPAIPPLLANFRQRVGLVAATASTGGQRRAAVWSEPLPARPGDTVQL
                     RLLWQSLAPAEESYTVFVHLIDAANRPLLALDYTPLGGSAPTHLWIPKWLPGQRLLDP
                     YRLVLPADLPPGQYYIEVGLYEMVGERRLHMADPAGNLIGDRLILGPVDVSE"
     gene            complement(10771..>11852)
                     /locus_tag="PK829_01505"
     CDS             complement(10771..>11852)
                     /locus_tag="PK829_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU39919.1"
                     /translation="EIVHDMAPAAELYLLKISTDVDLDQAVTYAISQGVDVISTSLTF
                     LNVTPGDGTGKFAAMATRARAAGVLWVTAAGNYREQHWSGQWADADADGLHEYAPGVE
                     VNVFGPGNGTAYQIPAGLSLSVSIRWDDWTEVRQDLRLLLLRYNGTRFEIVASGNNPQ
                     TGQFGQRPTERVNFITNGSAAIYGVAIQRVSGNRAVYLQLLTPNRELDRRVTAMSLGD
                     LADAPDALTVGAVSINSPYAAADYSSEGPTTGPGGAPGGGRLKPDISAYAGVSTASYG
                     TRAFSGTSAATPHVAGAAALARNQRPAYGPAALQFYLEELSIDQGPACADTRYGYGRL
                     FLRLPSEPAKNKLFLPLLSRSTERC"
BASE COUNT         1847 a         3851 c         4302 g         1852 t
ORIGIN      
        1 cgctgccggc ggaagcagag cgcttcattg gcgaggtgtt atggcagctg gggcacacag
       61 ccgatgccgc gctggccttg ccggccgaaa gcctggactc actggcgacg gccacttcta
      121 ttaccacccg cgagcaagag gtgttgcggc tgctgagtga ggggctaacc aatcgccaga
      181 tggctgaccg gctgtgtgtc tccgtcggga cgatcaagac acatctgacc aacatctacg
      241 gcaagttggg ggttaattgc cgcgtgcagg ccgtggccga ggcacaggcg ttgcgggtgc
      301 tttagggggc gatggctccg gccagggcgg gaatggctgc cgggtcggcc agcagccagc
      361 gggccaactc gtcgtggggc aaatcgttgc gccagcggcg ataggtgtct tgcggcaggt
      421 cgcggtagaa gagggccacg acgacggccg cgggatcgtc ccagcggtag gcgtagtaga
      481 ccgtcgtcag cacgcccggc agcgcgccgt tcttatagcc gacgttgctg ccccactcca
      541 gattgttctc gaattgattg ggccactcca gatagcgccg cgcctgaaag ctgctgtcgg
      601 cattgctcaa cccgttgccg gccagcaggg ccatgagggc ggcataggcg cgggtcgtgc
      661 cgcgcggggc cagacgggcg gcgaagatgc gctgtgtgtc gatggacggc cgccggttgc
      721 gctcgcgcca ggcggcctca gcggcgcgga actccgcctg accggcatag gcgtcggcca
      781 gcaaggccac gtcgcggccg taggcggcct cgtcgtcgac gtaggccgcc agcgcggcag
      841 tgtcgttagc cagcgtccgg ctgtggttgg ccatcagcag aaattggccc aggaaggtgc
      901 agggcgcgct gtggctgccg gcggggttgt ccaaccccag aacgcgggcg gtgtcctcga
      961 tgcgcgcctg gcccagccgc aggtgtagat agtcggccgc ggcattggaa ctgtggcgga
     1021 tcatcatcca cggcacttcg gacaagggca cggccgggtt gtcgtctggc gagagaaggc
     1081 ggccctcggc gcgcaactcg gccagcgctc gccggtgcgc ccccaggtcg aagttgggca
     1141 ggtagaagcg ctctagctca gccagcggca cgatctccag cgggtcgagt tcgccggcag
     1201 cgaccgcttc gacgtaggca accagagtga tgatcttggc caccgaggcc aggggcatgg
     1261 gcgtgtcggc gttccagtag atgccttcgg cctcgcggcc gacctggtag gccaccagcc
     1321 caacgcttgc cggcgcgtcg cgcagttgct cgatggccgg ccaggccgcg gccggtacga
     1381 tggcgcgcag cgcggcttcg tcgctcaggt tgatgctaga cgccgccggg ccatcgactg
     1441 gcagcgccgg caggctgaga ccgcacggcg gcgacagcgg cccggcatag atgggcgttg
     1501 ccgtggccgc caggggcgtt gccgtggcgc gattctcctg aatcgcgttc ggcggcggcg
     1561 tggccggcgc ggccgtggcg gtggcgcgat tctcctgaat cgcgttccgc gccggcgtgc
     1621 tcgccggcgc aacctccgcc ggggcgcggc aggccgcgcc cagaagcaga atgatggcga
     1681 tgggcagcag ggtgaacggg cggcgcatag gcaaacggtc tagcgtaccg ccttgccgcc
     1741 caggcgcttg aggacgatga gggtcatgtc gtcgaactgc gccgtctggc cggtgtggtc
     1801 gcggatgttg tcggtgatct ggcggacgac atcggccgat gaccgccgcg ccgccgcgcg
     1861 cgccgccact tgcagccgct ccagcccgaa ctcgtcgaag tcctcgttga gggcctcggt
     1921 gatgccatcg gtgtagagaa tgatcgtttc gcccggccgc agggtgatgg tgtggctctt
     1981 catcgagatc tctggcagaa tgcccagggc gacgccgtgg ccgagcagcg gctggaagtt
     2041 gccgttgggc tgcaacagca gcggtgggtt gtggccggcg ttggcgtagg tgaagtgctc
     2101 tgtggccggg tcccagacgc cgtagaacac ggtcacaaac aggtcggagc gcgcctcgcg
     2161 gccgataatc tcattggccc gcaccagcac ctgagccggc tcggtgtggc tgaaggccac
     2221 ggcgcgcagg atggtgcgcg tcagggccat gaatagggcc gcgggcacgc ccttgtcggc
     2281 cacgtcggcg atggcgatgc cccacttgtc gtcgcccaga ggcaggaagt catagaagtc
     2341 gccgcccacc tgtcgcgctg cctgccagta ggtggccacg gagcagccgg gaatgctggg
     2401 gctgccgtcg ggcaggaaac tggcctggat ggtgtgggcc acgtctagct cctgctgcat
     2461 gcgggcgcgg tcggccgact cgcggtagag ctgattgttg acgacggccg tggccgcctg
     2521 ctgggcgatg ccgttgagga tattgatgcg ccggttggag aacggcgtcc ggcccaggcg
     2581 gtcgcgttgc aggccgacga tcttcgcccc caccagccgg ccgcgggcca ccagcgggaa
     2641 attgtaggcc gtggctgtgc ccagcaccgt ctccaaccag ccgggcacgc gcatggcata
     2701 gtaacccgcg cccggcagtc gcggctggct ggaatcatgg gtcagccccg gcgacatcgc
     2761 cagtaactcc tcgcggtcga tctccagcgt ctccaccagc ccgcgcccca tcgtgctgac
     2821 gccatagctc ggcccggcct gaaagatctg acggtcttcg tcccagacga ggataaagac
     2881 ggtatcgacg ccgaccagca gcggcaccag ccgcacaatg gtgtcaagga tctcgttcag
     2941 atcggtcaga ctattgaccg cctcggccac ctgcaagaga gcggtgttga cccaggcttc
     3001 ctcctgctgg gccatgcgca ggtaggtgct ctccaggccg gcggcggtct gccgggccag
     3061 attctgcaca agctcctcgc ggcgggcgtg gaacggagcc gcgccgctgg ccgattgggc
     3121 cggcgtgggg ccgtcgtcag cgccgtcgtc gaaatcgacc agcattgccc ccagcgtctt
     3181 gaccgggccg ctgaccagcg ggtaaaggcg cagacaggcg cgcgccggcg acacctgccc
     3241 ttccagtaat ggctgcgtgg cccacggctg cgtggcccac ggcggtagat gggtggtggt
     3301 caacggctca cggccgacgt ggacggcatc gagcgcattc cagtcgccga tggccagacg
     3361 cacattggca aaggcagcgt cctcgttgcc gcgtcgatcg accagcgtcg cgccggtgta
     3421 gctctcgctg tcctcgtccc acagcagaaa ggcgcatagc ttgaccccgg ccagcagcgg
     3481 ggccagacgc gccacggccg tcagcatctc ctccggctcg tcgtggcggc caatggcttc
     3541 ggcgacctgt aactgggcgg tggtgatcca ggctcgctcc tgttcgcggg atagctgctg
     3601 cgttttgtag atggcgctgg aaagctccgc cgccagcgtt tccagcacga cccgttcgtt
     3661 cgcgccgaag gcacccactt gcggactctg gacatcaaat acgcccagca actcgttgtg
     3721 gacgagcagg gggatggcga tctcagcctg ggtgtcgggg ttcaattccg gcggcacgcc
     3781 gtcgaggtgg gccaggaagc gctcgtcctc gcccgcgtta ttaacgacca ccgtgtggcg
     3841 cgtcgccgcg gccgtgccaa tgatgccccg tcctatcggc aggcgcagct gcggcccacc
     3901 gccgttggcc gcgtccagtt cgggaatggt gctggcctgg atgatggcgt cgcgtgtcag
     3961 cgggtcaagg ccgaagaccg tcacagggtg gaagccgaac gtctggcggg tcagactgac
     4021 agcttgctcc agcacgtcat cgaggtcgtc ggcggcgttc acctggcggg cgatctgcgt
     4081 caccagctcc aggtgggcgg cgcgggtgcg ctcctgggca aagagccggg cattggcgat
     4141 ggccgcggcg gcctggttgg ccaggatggt cagccggcgc atgtcctgct cgctgaagtg
     4201 gtcgggcgtg gtgctctggg cggagacgat gcccagcgtg cggccgccgc tgaccaaagg
     4261 caggaagagc gccgcgcggg gcggcgaatc gctatggtag gccgggcgcg cgggcagctt
     4321 gtccatttcg cgctggaagt cgcgcacgag cagcggctgt tgcgagtcgc gcacccagcc
     4381 gaccagcccg ccttcatccc tcatccttcc gccttcatcc ttgcccaggt caaaacactg
     4441 cggcacgggc tggggctggc catcgatggt ccagtagcga atgtcgtagt agctgtcctc
     4501 gaacaggccg acctggaagg tgcggttgtc gatgacctgc ccggcctgct cggcgatgaa
     4561 gcggcaaagg gcatcgacat ctagctcggc ggcgacgatg gcccggccgg cgctgctgag
     4621 agcttccaat tcggcgacgc gctcgaccag cgcctggcgc gagcgacggc ggcgcaacga
     4681 ccgggcgatc agcagtccag ccacgacaac aacgagcagg atggcggcag cgatgagaaa
     4741 cgattgcgtc tgggacgggg aaaacatagc cagggctgtc gcgcgtcagg gggcgcgtgg
     4801 gcggtgggcg gctcgccacc tctatccgat gggccgcctc aggaacgcac tccctgacgc
     4861 gcctgaccgc gggccgtggc gcagtcgtcg aggatagtga aaaccttgtc gaaaccgacc
     4921 atggcgaaga tctcctgcaa gcggttgttg aggccacaga gcgtcagcgt gccgtcgccg
     4981 gctttggcct ttcgccaggc gctgaccagg cagcgcaggc cgccgctgtt gatgtaggtc
     5041 gcgtgggtca gatcgacgac gagggcgacg tggccgttgt ccagcagagc gttgagcgtc
     5101 tcttccagct tgggcgtcag gctctggtca agccggccgc tgatgccgat cagccacgta
     5161 ttgctttggg ggagtttttc ttgccagacg ctcatgggtc gccatccttg tgtagctttt
     5221 tgaccatgac cagtcgattg ccgtggcgcc ggtcggccgt gtaggtaact tcgtccatca
     5281 ggttgcggat gaagtgcagc cccagcccgc cgatgcgcat ctggtcggcc agctcatcgg
     5341 cgggcatggc cgtgccgctg atggccggcg gtggcggcac atcgccaggg tcgaaagcct
     5401 gaccggtgtc gcgcaggacg atggtgaacc cgtcgtcctc cacggcatag ctgatagcga
     5461 tgtcgccgac gccctcatcg ccataggcat gctcgatgat gttggtcgaa gcctcgtcgc
     5521 agcacagttc cagatggaag atggtgttgg catcgaagcc ggcatcggcc gcgccggcgg
     5581 cgatgaactc gcaaatccgc ttgatttcct tatatcgtcc ggggacgacc aggacttgtt
     5641 cacgtgccat atcgcccgat gccatatcgc gcccgctacc ggttggcaag taaaagtcgg
     5701 cacgggcgcg aagcgcccgt gccgccgtgg gttgagaatc gaaaactaga agctgccgac
     5761 cgccgccgtc tcgctgtcga aggtcttgaa gaccgtgttc agcccggcca gggtcagcac
     5821 ttcgacgacg cgctcggacg ggttcgccag caaaacgtca ccgcggtgct tctggcactc
     5881 gcgccagagg gcgacgatgg cccgcaggcc cgcgctgctg atgtactcga cgccgctcag
     5941 ttccagaacg acattttgct tgcggctgcc gacttccttc aggacgcgat cgaactcggc
     6001 cgcgttgctg ctgtcaatac gcccggtgac gcgcaccagt tccacgcgct tcaagtcctc
     6061 aacctgaata tctaccaacg attcggccat gttcacctgc ctcctggcta tgctaaagtg
     6121 tcccgactgt ggcccggctt gcgccgcggc ccggccaggg atcgctcatt aagcagcccg
     6181 attatagcat aggataaagg caaaacgtca atcgcgggca ggaatggtat cattccgagg
     6241 atatgcagcc ccccaccgcc atgctcacgg ccgaattaaa agcccaggcc gccgctatgg
     6301 gcttcaaccg cgtcggcgtc atccccgccg cgccggcccg ccgcctcgat gcctatctgg
     6361 cctggatcga cgccgggctg cacggcgaga tgggctacat ggcccggccc gaccgcgtcg
     6421 cccgccgccg cgaccccgcc gtgattctgc ccggcgtccg ttcgctggtc gtcgttggcc
     6481 tcgactacgc ggccccgccg ctgccggccg agatcgccgc ccggctggcc gacgacccgt
     6541 cccgcggccg cttctccaat tacgcctggg gggcggacta ccacgacgtc atgacgccgc
     6601 ggctggaggc gctgggcgac tggctgcgcg ggcagacggc cggcgaggtc gccagccgcg
     6661 cctacgtcga caccggcgcg ctgctggagc gcgaccacgg cgagacggcc gggctgggct
     6721 ttaccggcaa gaacacgctg ctcatcgacc cgcgggctgg atcgtggttc ttcctgggcg
     6781 tgctgctgac gacgctaccg cttgactacg accagccgtt ggtggcgtcg ccgtcgtgcg
     6841 ggcgctgccg gcgctgcctc gacgcctgcc ccacggccgc ctttcccgcg ccctatgtcc
     6901 tcgacgcgcg ccgctgcatc tcctacctga ccatcgaact gaagggctgg attccggccg
     6961 agctaaggcc gctgatgggc aactgggtct acggttgcga cgtttgccag gcggtttgcc
     7021 cttttaaccg cttcgccgcg ccggcggccg aggccgcctt tcgcccggcg tcgtgggatg
     7081 cggtcgcgcc gccgctgcta gatctgctgg cgctagacga cgaaggcttc cgccgtcgct
     7141 tcgcccacgg gccagtgggg cgcattggcc gggcgcgtct ggtgcgcaat gcctgcgtgg
     7201 ccgcgggcaa ctggggcagc gccgcggctg tggcggcgct ggccgcgctg ctgacggacg
     7261 ccgcgcccat cgtccgcggc catgccgcct gggcgctggg gcgcatcggc gggcaggcgg
     7321 ccggggcagc gctggccgcc gctctgccgg ccgaggccga tcccgacgtg cgccacgcgc
     7381 ttagcgcggc gactcagccg cgccgcgaaa cgcgcggata ttgaacaggg gcgtcgtcgt
     7441 catgatgacg cagcccgtgc ccagcaccag ccgccggtcg ccggtctgcg ctcgcgcctc
     7501 agccgcctcg gccaacaggt catccgactg gtcgcggtgg atcgtccact gcgacacgcc
     7561 gccgctggcc gcgccgggga agccggccag gccgtcgcgc agggagatgc ccgtctcgcg
     7621 gtcgtgccag ttgagcagcg gcgcggggta gtcggccacg agatcgaaca tgacccgcgt
     7681 gccgtgcagg tgaaccatgt tagcccacag gtcggcggcg ctgtccagca cgcgacggtc
     7741 gtagggccgg ccgaaggcag cgaactcgtc gcggctcagc agcgggtaaa cggcgtgctg
     7801 gacggcgtag tagatgccgg cgatgcccgt cgcccgggcc gcgtcgatga aggtgatgat
     7861 gctgtcggcc agcgtcgcca ggccgcggtg gagggcgtcg gggtgggcgc gcatgtggct
     7921 gagcagaacc tcgttgccgg ccaggtgctt ggccatcgac aggggcgaga agatggtggc
     7981 catgaagggc acttcgccgc gcagggtctg gccaataagt cgcagcgctt cgatttgcgt
     8041 cgccagcatg ccccggctgg ggtcagtcgg ccgcagcgct tcccagtcgg ttggctgcgt
     8101 cacgggatag cgcgtcgtcg tgcgcgtgcc ctcgatgtgg ccgtcccagc ggtcttcgac
     8161 gccccaatcg acgacagagt agctgctggc cgggccgacc ttgagcaggt cccagtcata
     8221 gtcggcctgc cacttcagat gggccgtggc cagggcggcg gcgtgctggt cgtcgccggg
     8281 ccagtgccgc cacagggcca cgggcggccg gtcggtcggc tcgccggaga tggttgcttc
     8341 cagtcgtttg cgtttggtcc agttggtcat ggtagctgtt gcctgtctta ctcgctgaca
     8401 tcgaccgggc cgaggatgag ccggtcgccg atgaggttac ccgcgggatc ggccatgtgc
     8461 aagcggcgct cgccaaccat ctcgtaaagc ccaacctcga tgtagtactg gcccggcggc
     8521 aggtcggccg gcagcaccag ccggtaggga tcgagcagcc gctggccggg cagccacttg
     8581 ggaatccaca ggtgcgttgg cgcggagccg cccagcggcg tgtagtcgag ggccagcagc
     8641 ggccggttgg cggcgtcgat gagatggaca aagacggtgt agctctcctc ggccggggcc
     8701 aggctttgcc acagcaggcg caactgcacc gtgtcgccgg ggcgggccgg cagcggttcg
     8761 ctccagacag cggcgcgccg ctggccgccg gtgctggccg tcgccgccac caggccgacg
     8821 cgctggcgga agttggccag caggggcggg atggccggct gctgcctggg gacggccaca
     8881 atcaattcgc ccaggggcaa ttccggcccc gtctcgtcgg tgctcaggtt gcgcagggcg
     8941 accgtgaccg ggtagacgcc gccgggcagg tcgtggggca gggcgaagtc gaagcgctcg
     9001 acgatcacct cgcccggctg ccactggggc gagatgagat ggctgtcggt ggtaaacggg
     9061 aagctcagcg cgccgacgcg gacgacgggg gcgtagtagt cggccgtgcc cgccggggcg
     9121 cgcagggcca gcgtcagggg cacgtagtcg ccggcggtga cgctgcgctg cggcagagcg
     9181 tagccggcga gctgggccgc gccctcgccg gccagcgggg tcaggtcgga cggcaggctg
     9241 gcgtcgcccg gctcgacgac gcggtagaac gggccgtctg gccgcaggcg aaagccggcc
     9301 gcgacgatct cgcggcggta gttgctcagg tagaccggcc cgccgggcag ggtgtcgaag
     9361 acgctttcca gccacggccg ggccgtagag acgaggatgg ggcgcacgtc ggcctcgtcg
     9421 ggccagcgct cgtcgacgaa gcgggcgtac cagagcgggg tcatgtgttc ccagtcattg
     9481 agcagggtcg cgccctcggc cgtgccggcg aagcggtcga agacggcatt cgcatagtcg
     9541 gcggcagccg tctcgtcgcg cagggagatg gccggcgcgt tgcgggccac ttgcagcccc
     9601 ggccccagca ggaagagcgc cgcggccagc agcgccagca gcgccggggc cggccgcagc
     9661 cgcgtcgccg tcagcgccag cagaccgtag aggcccagac cggccagcac agccaggatg
     9721 accagcagcc ccagcaggta ggccatgatg tcctgtgccc gcaggctcat gacgaaggcg
     9781 tagttgccgg ccagggcgag gacgtacagg gtcgtcagct tccacgcggg cacggccgcg
     9841 ccgccgcgcc gtcgcgccaa cagcggccaa accacgccca acatggccag aaagatcacc
     9901 ggcagcgaga actgtaagcg cagtagcgac cagaagacca gcgcccgtgc cggcgcgtcg
     9961 gccagtccga agaagggcag gctctcgctc aggccgcggg ccagaacatg gtcgaggaag
    10021 ccgctcagtg tggtcatcgt cgccgggcca aagggcgggg ccgtggcgct gcggatgggg
    10081 aagtagagcc agacggccag ccccagcacg gcgcagccgg ccatgcccag ccagacgcgc
    10141 cattcgcgcc agatgcgcgg ccgggccagc agaatgaaga cggcataggc ggggaaggac
    10201 atcaccgtca gcgggtggtg ggtgacgccc agcccggccg agagggcgaa ggcgtagagc
    10261 cagcgcggcc gagcggttcc gccggcggcc cagcgcgtca ggaaaaagag attggcggcc
    10321 aggaaagcgg cggtgacgat gtgggggttg gcgtggatgg cgtgctgcca gacgtcggcg
    10381 gccagaccat agctgagcgc ggcgaagacg gcggcgaccc tgtcccaggg cgcgtggagg
    10441 gccggcagca gcatccgcgc cgcggcgcgc actgtgccgt agatgaacag tgccgtccct
    10501 gtggccacgg cggccgatag caggtgcatc cgccaggaga cttcgcccag cggcacgagc
    10561 gtctgaaaca gcttgcccag cagtgtgtag aaggcgtagc cgggcgggtg ggcaatgccc
    10621 aggatgtggg ccacgaaggt gtattcggtc ggatcgccga agacgactga ccgcgccagc
    10681 gttagcacgt aggccagggg cagcagcagc agggcgagtg gcagcagcgc cgcggggttc
    10741 gtcgggcgca ggcggcgatg gctcatggta ttagcagcgc tcagtgctgc gagacagcag
    10801 gggcaggaaa agcttgtttt tggccggctc ggacggcagg cgcaggaaaa gccgcccata
    10861 gccgtagcgt gtatcggcgc aggccggccc ctggtcgatc gacagctctt ccaggtagaa
    10921 ctggagcgcc gctggcccgt aggctggacg ctggttgcgc gccagggcgg ccgcgccggc
    10981 aacgtggggt gtggccgccg aggtgccgct gaaggcgcgc gtgccatagc tggccgtgga
    11041 cacgccagcg taggcgctga tgtcgggctt cagccggccg ccaccgggcg cgccgcccgg
    11101 gccggtcgtc ggcccctcgg agctgtagtc ggccgccgcg taggggctat tgatgctcac
    11161 cgcgcccacg gtcagcgcgt cgggcgcatc ggccaggtcg cccaggctca tggccgtcac
    11221 gcgccgatcc agttcgcggt tgggcgtcag cagctggagg tacacagccc ggttgccgct
    11281 gacccgctgg atggccacac cgtagatggc ggcgctgccg ttggtgatga agttcacccg
    11341 ctcggttggc cgctggccaa actggccggt ctgcgggttg ttgcccgagg cgacgatctc
    11401 gaaacgcgtg ccgttgtagc gcagcagcaa caggcgcaag tcctggcgca cctccgtcca
    11461 gtcgtcccag cggatggaca ccgacagaga cagaccggcc ggaatctggt aggccgtgcc
    11521 gttgccgggg ccgaagacgt tgacctcgac gccgggggca tattcatgca ggccgtcggc
    11581 gtcggcgtcg gcccactggc cgctccagtg ctgctcgcga taattgcccg cggccgtcac
    11641 ccagagcaca ccggcggcgc gtgcccgcgt cgccatggcc gcgaacttgc ccgtgccgtc
    11701 gccgggggtg acgttgagga aggtcagcga ggtagagatg acatcgacgc cctggctgat
    11761 ggcgtaggtc acagcctggt cgaggtcaac atcggtggag attttgagca agtatagctc
    11821 ggctgccggg gccatgtcgt gaacgatctc gg
//
LOCUS       DAOSMQ010000023        11762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5421_length_11762_cov_4.701802, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000023 DAOSMQ010000000
VERSION     DAOSMQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11762
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5421_length_11762_cov_4.701802"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3441)
                     /locus_tag="PK829_01510"
     CDS             complement(<1..3441)
                     /locus_tag="PK829_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOU39920.1"
                     /translation="MLDDLELQQVQTIEVDGDQVWVQHGIPALEGDFLQGLGRRASQI
                     TLTGVLTGAEVADSLKKLRDKFRAAAPVAFVADIATAIRLDQVLIEEMGVRELAGRPA
                     RFEYAFTLREFKPATPTEIIIPPPPPPPPPPRTLTSVLEVQVIVEGDPNFDHALTRVL
                     VDGRDEAGQSVRRELPNDPSVRQGDLWTVDDFPAGSYTATATTRSPAMTGSASVTLPL
                     GQTRRAIIILRPGQTIATAFVIHYTFDRAFVEPCMRAVLRQVADFATAHADQKIIIVG
                     HTDESGDDNYNQSLSERRARGAHAFLTFGRDAQAADDAVAEWNELRRTRPTGETPSVH
                     DSWGAREYQYMLQDLGFYNGQVHGTHDAATDTAVRDFQTANGLAVSGQVDDLTWPVLI
                     RAYMAQDPLNVPASQFMPNADPADGCDNGVVRWLGCGEKLPVKSTPRGACGDPAWRPN
                     RRTELLFVRAAAFPCEIAKPVTFDLAPPGGAGWCLGPGNSKARCCFLTFEAGNQNSFL
                     VQPAEPGTFVVTGRIQFEDGTPLANTEYVLIAPDGEFMNGEVTCSDPSGARKGTPRRG
                     RTDANGNFTDKAGNVGYPQNPKGPGIYTLTIAADVVARNQGEPIESATGPNVCVRLDD
                     DGGFVVIVVGRAVVNVRPNIAPAGNVVVVRKPHTNPQRQPVVLSVNQAFTGSGTFTRS
                     SDAIRFFDAAVGGNEIAFNGTDNVFTDAQLVAGQTLFVEGSRASAAVDDVELRLVLTV
                     NGTPGLGATARMTALELTLDICQSRTAPGVDPAPLSAADKINPGRPLQTPNLANTQQR
                     ALVIVRSAQPAAFVGDLALTPLNGSVQLFSAADEVPAAGQAPLAGAQILANAAIPASG
                     ARFWAQGATASAAARDTGFQLSLQGVEPDGDRVAITVIQLDIVAAAADDSPTATFVRF
                     GLWDDAFDPATGNLRNNAAEAQNFVGSDSRHFFFRLRDASRAGTATIRWRTLFANNTL
                     DDPRTPAGNQDLTVTETAAGSGVFLSRAVMLVTDDVDRGQATDSGLTAGDVGPRNQGQ
                     SNHRLRCVTVDDTHQLDGRVEAEYAPAGVGVSPFTAPTLLFNRAPEERLRIRIHFINV
                     RNRVGGIGVLTAIRRAQVTQAFQALYALCGVFVEVDEITIDPPAAATGWPAAFPGDPL
                     A"
     gene            complement(3510..3926)
                     /locus_tag="PK829_01515"
     CDS             complement(3510..3926)
                     /locus_tag="PK829_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39921.1"
                     /translation="MNTNIFEELKNLLTEFQTFLEQNVATIRPAILAIAGVFPQIVEL
                     IDKLIELMNKLKTEINNFDLGNINGLTEATQFINMIKSFLESARTLLPDEAGTINDVL
                     AVADVVGSLGGLTDVKTDILKAIDTIVTQLASLKPA"
     gene            complement(3982..4527)
                     /locus_tag="PK829_01520"
     CDS             complement(3982..4527)
                     /locus_tag="PK829_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39922.1"
                     /translation="MRPMLDDIELPQVQEITAYDRRMLAEHKPPGMDGSLLQNLGRRP
                     AHLTLWGVKTGPDALEFVETLELKYRAGQAVPFVADIIADAEIQQVVIDDLQWQELAG
                     RPQRFSYVVALREFIEPVEPEDTTALQEDILGDAGDLFDDLLDGLDFALPFATGLERF
                     LGPLTDILGRLQQLNRPAGNP"
     gene            complement(4581..>5467)
                     /locus_tag="PK829_01525"
     CDS             complement(4581..>5467)
                     /locus_tag="PK829_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39923.1"
                     /translation="GGSFPGVGSRRATPELLARLAGRGAKAGPRTSEVKRPPLARSKG
                     QAATAAGQSAAGQEWAGRLAERVRQRALAGLPAGEPRDANERGHFPAPAERPSDLPAQ
                     WREPLDGQRVPAGWLAATQASTPATRPPAAPTGQPRARATSNDAPPLSSPSGHRPILH
                     PPQRGRRSPAGRRDDPTGAARGLSRPVPAGGDSDGWVGPGPDVDGASHRNGRLEADNG
                     EVVSVVNRFAPPQMADTLPPLRPPVGQGSVAMPVAAAASRRSARREEAAVVDDLDELA
                     VKLRRILEEESRRHGIDI"
     assembly_gap    5468..5567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5568..5969)
                     /locus_tag="PK829_01530"
     CDS             complement(<5568..5969)
                     /locus_tag="PK829_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39924.1"
                     /translation="MTRPRLCPPRPRVVFAGGRAPACWRQQVRPTAAAPAALARLCGW
                     MAAMAQETGPKLRSEPLLGTCLAEMVGDGPAEAPSAVETTASGGGKPRPAEPGASRQA
                     QTATPPLAHETGKRRPATAPVTGERRQPLPGG"
     gene            complement(5966..6436)
                     /locus_tag="PK829_01535"
     CDS             complement(5966..6436)
                     /locus_tag="PK829_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39925.1"
                     /translation="MTLTAEELLAGSTLTHRVEIPADLLGSNGHRPVDTEVVLRPLTV
                     QALQRIGKAAGDDETLSASLMIQGALVEPALRFEQVAQLPAGAARFLVERINAISGIT
                     TARDELEELVQAPLARACFVLAKTFGWTPEEVSGLTIGQILLYLEMARRDGGPA"
     gene            complement(6513..7076)
                     /locus_tag="PK829_01540"
     CDS             complement(6513..7076)
                     /locus_tag="PK829_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39926.1"
                     /translation="MALTNVYTGANGTLTLAFEDTPEGNDAKAVIDTYQITTVGRVAN
                     VEVCIQTDLEEFHEIGRRHATSLHPGNVHISGRVGRAYINGALLFLLMGRGAAPTQIA
                     EPFVQPTFTMRVALNDPAVPGNTVSLELKGVKFQNWGVAMPEDDFVMENLSFKALTIS
                     VVDQEAPAGGGDAVAVVPEGFAGVDQA"
     gene            complement(7121..>8185)
                     /locus_tag="PK829_01545"
     CDS             complement(7121..>8185)
                     /locus_tag="PK829_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HOU39927.1"
                     /translation="APAAADDVVTVSYAVPAASSAKVTLKLGTAQEVYVIANGNDLAR
                     DINARSTLATATADATNGIELPTAFADAKDYHLFGSGTNTRGNNGADAGQDNYKNGLD
                     LLMNVSAHIIVAAGQSNDVIGDELAQHVADASDDMNKRERIGIVGSALGATFDDIRGH
                     NLNSDRIVFVAPGIITYDAALKKDVTLPGSYSAAAIAGLMSSYDPHVSLTNKPVPVAG
                     LEHKFTNPELAVLLEEGRVLALQEKLGFKVVRGITTSTNTAWKQITTRRIVDFAKYGV
                     RSAADPYIGMLNNERVRGALRATINSFLAEMVEDEMLIGYDLTVSATRDQEIKGICAV
                     TMVVRPVFSIDFIKVTMFLA"
     assembly_gap    8186..8285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8286..8671)
                     /locus_tag="PK829_01550"
     CDS             complement(<8286..8671)
                     /locus_tag="PK829_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HOU39928.1"
                     /translation="MSEMILPGVYIEVRPEALIVPGRVSVNTVGIVGTANRGPVDTPV
                     LLAGYSQAREVFGEYDAWDEDNPTALTLMRALELAFGHGATQVWAVRVANGAAAGSYV
                     LQSDGGANVTLSANSPGEWGNALEVNV"
     gene            complement(8790..10034)
                     /locus_tag="PK829_01555"
     CDS             complement(8790..10034)
                     /locus_tag="PK829_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39929.1"
                     /translation="MDRDAPPGHSFIIRMWPEELAVTAGVIQWRGHITHVPSGDRRYF
                     QDLDALRQFIQPYQGDMVSLFSFDDPADAAGSASAELAIAAARMTEALSTMLTRLREA
                     AALPDETDTLPAADATILSVEQKLVGLGHWRGQELRQHIAPVALKGGRLLATIQLQLW
                     GASPGDVGTLSTAVHGRLLGARELLQQQGFLRLRAAGASLAEHVSALDVWRKTLTYEV
                     LYEYHYQDADGAEGLIARIPIATDLETHDSPNRENNLVTDALARWDQLTAPALELRGV
                     QDVGRLHALVFFAGLPPTGSVEWRRTTSTTTAPPQEFATFDDFLAAITDPADPALNAR
                     FVFPTLIAFLDRFEPDPNLVSMGNWNADEDDVLDTYLARTLALARPVRLRRGIDRLRI
                     AYAPGGADPRFDTVGIVYLRAG"
     gene            complement(10181..11173)
                     /locus_tag="PK829_01560"
     CDS             complement(10181..11173)
                     /locus_tag="PK829_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOU39930.1"
                     /translation="MSVCLFGRFAAETDSRPVASLSGAKVQELFSYLLLYRTRPHRRE
                     ALTTLLWPDVPDVQSKRYLRKTVWQLQAALAQLTPPDAPPILHVDPDWLQVSAKVDSW
                     LDVAQFECSYGLAEDTAGEQMTPDTAARVEAAVELYRGDLLEGWYQEWCLFERERLER
                     MYLSLLDKLISHCAAHGRYEVGIGYGTRALRHDPARERTHRRLMRLHALAGNRSEALH
                     QYALCVDTLRRELGVTPSERTNALYQHILTAGDETEASDLAPVERPQPMPEGDRPLPA
                     PNGEVNHALARLEQMLRLLGETQRLVQQNMATVAATLDDLRRDDLRRDDLRHRA"
     gene            complement(11443..>11762)
                     /locus_tag="PK829_01565"
     CDS             complement(11443..>11762)
                     /locus_tag="PK829_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU39931.1"
                     /translation="ISRGSIFKLPIVAYADAAALLTELHRLGVASVATTANDGAPLPR
                     LARPPGPVAVLMGNEAEGLPAEVATTVDIRVTIPMAAGVDSYSVNAAAAIVLYHLRQE
                     ASA"
BASE COUNT         1709 a         3769 c         4136 g         1948 t
ORIGIN      
        1 ggccagtggg tcgccgggga aggccgccgg ccagcccgtc gccgccgccg gtgggtcgat
       61 ggtgatctcg tccacctcca cgaaaacgcc acagagagcg taaagcgcct ggaatgcctg
      121 ggtgacctgt gcccgtctga tggctgttag cacaccaatg ccgccgacgc ggttgcgcac
      181 gttgatgaaa tgaatcctga tccgcaggcg ctcttccggc gcgcggttga agagcagcgt
      241 cggcgcggtg aacgggctga cgccgacgcc cgccggcgcg tactccgcct cgacccggcc
      301 gtcgagctgg tgagtgtcgt caaccgtcac gcaacgcagg cgatggttgc tttgtccctg
      361 gttgcgcggg ccgacgtcgc cggccgtcag gccgctgtcg gtcgcttgcc cacgatccac
      421 gtcgtcggtc accagcataa ccgcgcgcga gagaaacacg ccgctgccgg cggccgtctc
      481 ggtgacggtc aggtcctggt tgcccgccgg tgtgcgcggg tcgtccaggg tattgttggc
      541 gaagagcgta cgccagcgga tggtcgccgt cccggcgcgg ctggcgtcgc gcagacggaa
      601 gaagaagtgg cgcgaatcgg agccgacgaa gttttgcgcc tcggcggcgt tgttgcgcag
      661 gttgccggtg gccgggtcaa aggcatcgtc ccacaggccg aagcggacga aggtggctgt
      721 cgggctatcg tcagccgcgg cggcgacaat gtctagctgg atgacggtga tggccacccg
      781 gtcgccgtcc ggctctacgc cctgcaagct caactggaag ccagtatcgc gggcagcggc
      841 gctggccgtc gccccctgcg cccagaagcg cgccccactg gcggggatgg cggcattggc
      901 cagtatctgc gcgccggcca gcggcgcttg cccagccgcc ggaacctcgt cggctgcgct
      961 aaacaactgc acgctaccgt tgagcggcgt cagcgcgaga tcgcccacaa aggccgccgg
     1021 ctgggcggac cgcacaatga ccagcgcccg ctgctgggtg ttggccagat tcggcgtctg
     1081 gagcggccgg ccggggttga tcttgtcggc ggccgagagc ggtgcggggt caacgcccgg
     1141 cgctgtccgg ctctggcaga tgtccagcgt cagttccagc gccgtcatcc gcgccgtcgc
     1201 ccccagtccc ggcgtgccgt tgaccgtcag caccagccgc agctcgacgt cgtccacggc
     1261 cgcgctggcg cggctgccct cgacaaagag ggtctgcccg gcgacgagtt gggcgtcggt
     1321 gaagacgttg tccgtgccgt taaaggcgat ctcgttgccg ccgacggcgg cgtcgaagaa
     1381 gcgaatggcg tcgctggaac gggtgaacgt gccgctgccg gtgaaggcct ggttgacgct
     1441 cagcacgacc ggctgccgct gcgggttggt gtgcggcttg cgcacaacga cgacattgcc
     1501 cgccggggcg atattgggcc gcacgttgac gaccgcgcgg ccgacgacga tgaccacaaa
     1561 gccgccgtcg tcgtcgagac ggacacagac atttggcccc gtggcggact cgatcggctc
     1621 gccctggttg cgcgccacca cgtcggcggc gatggtcagg gtgtagatgc ccggcccctt
     1681 ggggttctgg ggatagccga cgttgcccgc tttgtcggtg aagttgccgt tggcgtcggt
     1741 gcggccgcgc cggggcgtgc ccttgcgcgc gccgctgggg tcgctgcacg tcacttcgcc
     1801 gttcatgaac tcgccgtccg gggcgatgag gacgtactcg gtgttggcca gcggcgtgcc
     1861 gtcctcgaac tggatgcggc cagtgacgac gaacgtgccc ggctcggccg gctgcaccag
     1921 gaagctgttc tggttgcccg cctcgaaggt caggaagcag cagcgcgctt tggagttgcc
     1981 cggccccagg caccagccag cgccgcccgg cggggccagg tcgaaggtca ccggcttggc
     2041 gatctcgcag gggaaggccg ccgcgcggac gaagagcaac tccgtgcgcc ggttcggccg
     2101 ccaggccggg tcgccgcacg cgccacgggg cgtcgacttg acaggcagct tctcgccaca
     2161 gcccagccag cggacgacgc cgttgtcgca gccatcagcc gggtcggcgt tgggcatgaa
     2221 ctggcttgcg ggcacgttca gcggatcctg ggccatgtag gcgcggatga gcacgggcca
     2281 cgtcaggtcg tccacctgac cactgacggc caggccgttg gcggtctgga agtcgcgcac
     2341 ggccgtgtcg gtggcggcgt cgtgcgtgcc atgcacctgg ccgttgtaga agcccaggtc
     2401 ttgcagcatg tactggtatt cgcgcgcgcc ccaactgtcg tggacggagg gcgtctcgcc
     2461 ggttggccgg gtgcggcgca actcgttcca ctcggccacg gcgtcgtcgg ccgcttgcgc
     2521 gtcgcggccg aaggtcagga aggcgtgcgc cccgcgtgcc cgtcgctcgg aaagggactg
     2581 gttatagttg tcgtcgcccg attcatcggt gtggccgacg atgatgatct tctggtcggc
     2641 gtgcgctgtg gcgaagtcgg ccacctggcg caggacggcg cgcatgcacg gctccacaaa
     2701 ggcccggtcg aaggtgtagt ggatgacaaa ggccgtggcg atggtctggc ccgggcgcag
     2761 gatgataatg gcgcgccggg tctggcccag cggcagggtc accgacgccg agccggtcat
     2821 ggccgggctg cgcgtggtgg cggtcgcggt gtagctgccc gcggggaagt catcgaccgt
     2881 ccacagatcg ccctggcgca cggacgggtc attgggtagc tcgcggcgga cagactgccc
     2941 ggcctcgtcg cgcccatcga cgaggacacg ggtgagggcg tggtcgaaat tggggtcgcc
     3001 ctcaacgatg acctgcacct ccagcaccga cgtcagcgtg cgcggcggcg gcgggggcgg
     3061 cggcgggggt gggatgatga tctccgtcgg cgtggccggc ttgaactcgc gcagggtgaa
     3121 ggcgtactcg aagcgcgccg gccggccagc cagctcgcgg acgcccatct cctcgatgag
     3181 cacctggtcg aggcggatgg ccgtggcgat gtcggcgacg aaggccaccg gcgcggcggc
     3241 gcggaacttg tcgcgcagct tcttcaggct gtcggccact tccgcgccag tgaggacgcc
     3301 ggtgagggtg atctggctgg cgcggcggcc cagaccctgg aggaagtcgc cctccagggc
     3361 cggaatgcca tgctgcaccc acacctggtc gccatcgacc tcgatggtct gcacctgctg
     3421 taactcaagg tcgtcgagca tcggccgggg cacggttcac ttctcctgcg tttgcctggt
     3481 gaagctggtc agtctcgttc tggcgctgat tacgccggct tgagcgaggc cagttgggta
     3541 acgatggtgt cgatagcctt gagaatgtcg gtcttgacgt cggtcaaacc gcccaggctg
     3601 ccaacgacat cggccacggc gagcacatcg ttgatcgtcc cggcctcgtc cggcagcagc
     3661 gtgcgcgccg actccagaaa cgacttgatc atgttaatga actgggttgc ctccgtcagg
     3721 ccattgatgt tgccgaggtc gaagttgttg atctccgtct tcagcttgtt catcagctcg
     3781 atgagcttgt caatcagctc gacgatctgc gggaagaccc cggcgatcgc cagaatggcc
     3841 ggccggatgg tggcgacgtt ttgttcaagg aaggtctgga attctgttag aaggtttttt
     3901 agttcttcaa agatgttggt gttcatcata tgctccttac actgtcaagg ctggctgatt
     3961 gccagacggc ggccgggctg tctacgggtt gcccgccggc cggttcaact gctgcaaacg
     4021 gcccaggatg tcggtcagcg ggccgaggaa gcgctccagc ccggtggcga agggcagggc
     4081 gaaatcaagg ccgtcgagca ggtcgtcgaa caggtcaccg gcgtcgccga ggatgtcctc
     4141 ttgcagcgcc gtggtgtcct ccggctcaac cggctcgatg aactcgcgca aagccacgac
     4201 gtagctaaag cgctgcggcc ggccggccag ctcctgccat tgcaggtcgt cgatgaccac
     4261 ctgctgaatc tccgcgtcgg cgatgatgtc ggccacgaag ggcacggcct ggccggcgcg
     4321 gtacttcagc tccagcgtct cgacgaactc cagcgcgtcc ggcccggtct tgacgcccca
     4381 gagcgtcagg tgggccggcc ggcggccgag gttctgcagc aggctgccgt ccatacccgg
     4441 cggcttgtgc tcggccagca tgcgccggtc gtaggccgtg atttcctgca cctgcggcag
     4501 ttcgatatcg tcgagcatcg ggcgcatggc cttcactcct gggttgccgg cggggcgcac
     4561 actggccgtg cgccgcgccg ctaaatgtcg atgccgtggc ggcgcgattc ttcttccaga
     4621 atccgccgca gcttgacggc caactcatcg agatcgtcta cgaccgcggc ctcctctcgc
     4681 cgcgcgctgc gacgggacgc ggcggcggcg acgggcatgg ccacgctgcc ctgcccgacc
     4741 ggcgggcgca agggcggcaa cgtgtcggcc atctgcggcg gcgcgaagcg attgaccacg
     4801 ctgaccacct cgccgttgtc ggcctccagc cggccgttgc ggtgcgaagc gccgtccacg
     4861 tctggccccg gcccgaccca gccgtcgctg tcgccgccgg cggggacggg gcgggatagt
     4921 cctctcgccg cgccggtggg gtcgtcgcga cggccagccg gcgaccggcg tccacgctgg
     4981 ggcggatgca ggatcgggcg gtgtccgctc ggactcgata acggcggcgc gtcgttgctg
     5041 gtcgcacggg cgcgcggttg tccagtgggc gcggccggcg gccgggtcgc gggcgtgctc
     5101 gcctgggtcg cggccagcca gccggcgggc acgcgctgcc cgtccagcgg ctcgcgccac
     5161 tgagccggca ggtcgctggg ccgctcggcc ggggcgggga aatgcccgcg ctcgtttgcg
     5221 tcccgcggct cgcccgcggg caagcccgcc agcgcccgtt ggcgcacgcg ctcggccagt
     5281 cgcccggccc actcctgccc ggcggctgac tggccggctg ctgtcgccgc ctggcccttt
     5341 gaccgggcca ggggcgggcg cttgacctca gacgttcgcg gcccggcctt cgcgccgcgc
     5401 ccggccagcc gggccaatag ctccggcgtc gcccgccggc tgcccacgcc ggggaaagag
     5461 ccgccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gccgggcagc
     5581 ggctggcggc gctcgcccgt caccggggcg gtggcggggc ggcgcttgcc cgtctcatgg
     5641 gcgagagggg gcgtggccgt ctgcgcctgt cggctcgcgc cgggttcggc cgggcgcggc
     5701 ttgccgccgc cactggccgt cgtctcgacc gcgctcggtg cttccgctgg cccgtcgccg
     5761 accatctcgg ccaggcacgt gcccagcaac ggctcagagc gcagcttcgg gccggtctcc
     5821 tgggccatcg cggccatcca gccgcagagg cgcgccagag cggccggcgc ggcggccgtg
     5881 ggccgcacct gttgccgcca acaggcgggg gcacggccgc cggcaaagac gacccggggc
     5941 cggggcgggc agaggcgcgg tcgcgtcacg ccggccctcc gtcgcgccgg gccatctcca
     6001 gatagagcag gatctggccg atggtcaggc cgctgacttc ctccggcgtc cagccgaacg
     6061 tcttggccaa cacgaagcag gcccgcgcca ggggcgcttg caccagctcc tccagctcgt
     6121 cgcgggcggt ggtgatgccg ctgatggcat tgatgcgctc caccaggaag cgggccgcgc
     6181 cggccggcag ctgggcgacc tgctcgaacc gcagcgccgg ctcgaccagc gccccctgga
     6241 tcatcagcga ggcggagagg gtctcgtcgt cgccggcggc cttgccgatg cgctgcaacg
     6301 cctgcaccgt tagcgggcgc agtaccacct cagtatcgac cgggcggtgg ccgttgctgc
     6361 ccagcaggtc ggccggtatc tccacacggt gggtcagcgt gctgccggca agcagttctt
     6421 cggcggtcag ggtcattgtc tgtctccgtc tgggccttgc cagacctcag aggcttactg
     6481 aaacctctga ggtcttagcg ggccaagctc atctaggcct gatcgacccc ggcgaagcct
     6541 tcgggaacca cggccacggc gtcgccgccg ccggccggcg cttcctggtc aaccacgctg
     6601 atggtcaggg ccttgaagct gaggttctcc atgacgaagt cgtcctccgg catggcgacg
     6661 ccccagttct ggaacttgac gcctttcagt tccaggctga cggtgttgcc gggaacggcg
     6721 gggtcgttca gggcgacgcg catcgtgaac gtgggctgga caaacggctc ggcgatctgc
     6781 gtcggcgcgg cgccacggcc catcagcagg aacagcagcg cgccgttgat gtaggcccgg
     6841 ccgacgcgcc cgctaatgtg gacgttgccg gggtggagcg aggtcgcgtg gcggcggccg
     6901 atctcgtgga actcctccag atcggtctgg atgcagacct cgacgttggc cacgcggccg
     6961 accgtcgtga tctggtaggt gtcgatgacc gccttggcgt cgttcccttc cggtgtatcc
     7021 tcgaacgcca gcgtcaacgt gccgttggcg ccggtgtaga catttgtcag tgccatgaga
     7081 cttgtctcct tgtctatctg ctaatctgcc tgccccgcgc ctaggcgagg aacatcgtca
     7141 ccttgatgaa gtcgatgctg aagaccggcc ggacgaccat cgtcacggcg cagatgccct
     7201 tgatctcctg gtcgcgcgtg gccgatacgg tcaggtcgta gccgatcagc atctcgtcct
     7261 cgaccatctc ggccaggaag ctgttgatgg ttgcccgcag cgcgccgcgg acacgctcgt
     7321 tgttgagcat gccgatgtag gggtcggccg ccgagcgcac gccatacttg gcgaaatcga
     7381 cgatgcgtcg ggtggtgatc tgcttccagg cggtgttggt cgaggtggtg atgccgcgca
     7441 cgaccttgaa gcccagcttc tcctgcaagg ccagcacgcg cccctcttcc agcagcacag
     7501 ccagttccgg gttggtgaat ttgtgttcca gaccggccac gggcacgggc ttgttggtca
     7561 ggctgacgtg tgggtcgtag ctggacatga ggccggcgat ggccgcggcg ctatagctgc
     7621 cgggcagggt aacgtccttc ttcagggccg cgtcgtaagt gatgatgccg ggggcgacga
     7681 agacgatgcg gtcgctgttg aggttgtggc cgcgaatgtc gtcaaaagtg gcccccagcg
     7741 cgctgccgac gatgccgatg cgctcgcgct tgttcatgtc gtcggaagcg tcggccacgt
     7801 gttgggccaa ctcgtcgccg atgacgtcgt tgctctgccc cgcggcgaca atgatgtggg
     7861 cgctgacgtt catcagcagg tcgaggccgt tcttgtagtt gtcctggccg gcgtccgcgc
     7921 cgttgttgcc gcgcgtgttc gtgccgctgc cgaacaggtg atagtccttg gcgtcagcga
     7981 aggcggtggg cagttcgatg ccgttggtcg cgtcggccgt tgccgtggcc agggtcgagc
     8041 gcgcgttgat gtcgcgggcc aggtcgttgc cgttggcgat gacgtagacc tcctgagccg
     8101 tgcccagctt cagcgtgacc ttggcgctgc tggccgcggg gacggcgtag gatacggtga
     8161 cgacgtcatc ggccgcggcg ggcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnacgtt gacctccagc gcgttgcccc actcgccggg gctgttggcg ctcagcgtca
     8341 cgttggcgcc gccgtccgac tggaggacat agctgccggc ggccgccccg ttggcgacgc
     8401 ggacggccca gacctgcgtc gcgccgtggc cgaaggccaa ctccagcgcc cgcatgagcg
     8461 tgagggcagt ggggttatcc tcgtcccacg cgtcgtactc cccgaacacc tcgcgggcct
     8521 ggctatagcc ggccagcagc acgggcgtgt cgaccgggcc gcggttggct gtgccaacaa
     8581 tgccgacggt attgaccgac acccgcccag gcacgatgag cgcttcgggg cgcacctcga
     8641 tgtagacacc tggcaagatc atctcggaca taggtttgct cctgtagaga aaactgggtg
     8701 gcggtcgggc cgccgctggc tattcctttt cgcgaccggc gcgccgcgcc caaaggcgcg
     8761 ccacgccgct aaccgcgggt cggggcgact tacccggccc gtagatacac aatgccaaca
     8821 gtatcgaagc gcggatcggc gccgccgggc gcgtaggcaa tccgcagccg gtcaatgccg
     8881 cgccgcaagc gcaccggccg cgccagcgcc agcgtgcgcg ccaggtaggt gtccagtacg
     8941 tcgtcttcgt cggcgttcca gttgcccatg ctgaccagat tggggtcggg ttcgaagcga
     9001 tccagaaagg cgatgagcgt gggaaagacg aagcgggcgt tcagcgccgg gtcggccggg
     9061 tcggtgatgg cggccaggaa gtcgtcgaag gtggcaaatt cctggggcgg ggccgtggtc
     9121 gtgctggtgg tgcgccgcca ctcgacgctg cccgtgggcg gcagcccggc gaagaagacc
     9181 agggcatgga gccggcccac gtcctgcacg ccgcgcagtt ccagcgccgg cgcggtcagt
     9241 tggtcccaac gcgccagggc gtcggtgacg aggttgttct cgcggttggg cgagtcgtgg
     9301 gtctccaggt cggtggcgat ggggatgcgg gcgatcagcc cctccgcgcc gtcggcgtcc
     9361 tgatagtggt actcatagag cacctcataa gtcagcgtct tgcgccagac atcgagggcg
     9421 ctgacgtgct ccgccagaga cgcgccggcc gcccgcagcc gcaggaagcc ctgctgttgc
     9481 agcaactcgc gcgcgcccag gaggcggccg tggaccgcgg ttgacagcgt gccgacgtcg
     9541 cccggcgacg cgccccagag ttgcagctgg atggtggcca acagccggcc gcccttcagg
     9601 gccaccgggg cgatgtgctg gcgcagttcc tgcccgcgcc aatggcccag gccgaccagc
     9661 ttctgctcaa cgctcaggat tgtggcgtcg gccgcgggca gggtgtctgt ctcgtccggc
     9721 agcgccgccg cttcccgcag ccgggtcagc atcgtgctca gtgcctcagt catgcgcgcg
     9781 gcggcaatgg ccagttcagc gctggcgcta ccggcggcgt cggcgggatc gtcaaacgag
     9841 aaaagagata ccatatcgcc ttggtaaggc tggatgaact gccgcaacgc atccaggtcc
     9901 tgaaagtatc gtctatcgcc actgggcaca tgggtgatgt gcccacgcca ctggatcact
     9961 ccggcggtta ctgccaactc ctccggccac atccggatga tgaaggagtg acctggagga
    10021 gcatctcgat ccatacgcgg gcagaatagc agccgtctgt atcccggacg gtatcccggc
    10081 gcgtatcacc ggccgaccag ttttggctca gtttggcggc gtggccagag agaggggcga
    10141 agggagagga aaggggcagc cgcagagacg ccgcgccggg tcaggcccga tgacgcaggt
    10201 cgtcgcgacg caggtcgtcg cgacgcagat cgtcgagcgt cgccgcgaca gtggccatat
    10261 tctgctggac gaggcgctgt gtttcgccca gcagccgcaa catctgctcc agccgggcaa
    10321 gggcgtgatt gacttcgccg ttcggcgcgg gcagggggcg gtcgccttcc ggcatcggtt
    10381 gggggcgttc caccggggcg aggtcagacg cttcggtctc gtcgccagcg gtcaggatgt
    10441 gctggtagag ggcgttggtg cgctccgaag gagtcacgcc cagttcccgg cgcagcgtat
    10501 cgacacagag agcgtattgg tggagcgctt ccgagcggtt gcccgccagg gcgtgaaggc
    10561 gcatcaggcg gcgatgggtg cgctcgcggg cggggtcgtg ccgcagcgcc cgcgtgccat
    10621 agccaatgcc gacttcatag cggccgtgcg cggcgcagtg gctgatcagt ttgtccagca
    10681 ggctgaggta catgcgctct agccgctcgc gctcaaacag acaccactcc tgataccagc
    10741 cttccagcaa gtcgccgcgg tagagttcga cggccgcttc cacgcgcgcg gccgtgtctg
    10801 gcgtcatctg ctccccggcg gtgtcctcgg ccaacccgta gctacactcg aactgggcca
    10861 catcgagcca ggaatcgact ttggcgctca cctgaagcca atcgggatcg acgtgcagga
    10921 tgggcggcgc gtcgggtggc gttaactggg ccagggccgc ctggagttgc cagaccgtct
    10981 tgcgcaggta gcgcttcgac tggacgtcgg gcacgtctgg ccagaggagg gtcgtcagag
    11041 cttcgcgccg gtggggccgt gtgcggtaca gcagcaggta gctgaaaagc tcctgcactt
    11101 tggcaccgct tagagaagcg accggccggc tatccgtctc cgcggcaaac cggccgaaga
    11161 gacagacgct caaagctgtc attccacgaa cttcctgagg cgtcggcggg ctgtcctgcc
    11221 atcgccatcg gtgacggaac ttcgatgtgt acttcgattt agtgatggtt cactggcacg
    11281 tgaacgcaag taggattcag tataatggct gcgattcact tgtcaatagg ccaggacgaa
    11341 tgtctcagag aggcgcggtc aataattctc tctgaagccg cgcgtagagg cagtcgtggg
    11401 tcattgacaa acagagacgg cgcagcgctc ggccgcccga gcttaagccg acgcctcttg
    11461 ccggaggtga taaagaacaa tcgccgcggc ggcattgacc gagtaggagt ctacgccggc
    11521 ggccatcggg atggtcacgc ggatgtccac ggtcgtggca acctcagccg gcagcccctc
    11581 ggcctcgttg cccatcagca cggcgacagg gccgggcggg cgggccagcc gcggcagggg
    11641 cgcgccgtca ttcgcggtcg tggccacact ggcaacacca agacggtgca actccgtcag
    11701 cagcgcggcg gcgtcagcgt aggcgacgat gggtagcttg aagatgctgc cccggctgat
    11761 gt
//
LOCUS       DAOSMQ010000024        11717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5450_length_11717_cov_4.412794, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000024 DAOSMQ010000000
VERSION     DAOSMQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_11717_cov_4.412794"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PK829_01570"
     CDS             complement(<1..465)
                     /locus_tag="PK829_01570"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOU39932.1"
                     /translation="MDIPNDQLRQFFINAFSDDELEDFCFDYFPAASQEFAPGMPVGR
                     KARLLISFADRRGQREHLLVALGKVRPEQFAEAFKKLPQLAAPPSASTTRLTRRVFIS
                     HAEEDVELAHRLATALREAGRPVWIAPDDILPGEQWVEAIGRGLTVSGLFVVL"
     gene            656..2428
                     /locus_tag="PK829_01575"
     CDS             656..2428
                     /locus_tag="PK829_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU39933.1"
                     /translation="MTIRIIIADDHSVVRQGLRMFLGLDPELEVVGEAVNGAEAVQKA
                     EALQPDVVLMDLIMPVMSGLEATAEIRRRGLAAQVIVVTSVIEEAAVTAVIRAGAIGY
                     LLKDTEADELRRAIKAAAAGRAQLSPAIAARLLAETQSSDADTLTDRENEVLRLAAAG
                     LSDQEIALQLLVSEPVVHSHVAGIVEKLRLANQSQTLLLDFANRAQTYLDPTALLDFL
                     VTEVPRLLQADAAALLLPEPGDGRLALRAAHNWPAGPLNETLGAGALSAFESRQPWQD
                     GRDDAAGPLASVAFIPLLIGGGAIGVLGLGGQTPRRLNEDELRFARLLANQAALSVEQ
                     ARLHRVESRGQRMERELQQARQIQMSLLPSEYPQVAGYDFAAAYESAREIGGDLYDFI
                     EWPGDPDRIGLLIADVSGKGTAAALFMAHSRAMIRGAAQTQPGPAATLAAANVQIARD
                     NHAMLFVSAFYAVLDAPSGRLTFANAGHNVPLVRRANGGVEEALARGMVLGIMDDMTY
                     DEATTTLAGGDIIVLYTDGITEAMNASGELFDKERLMAAIRGANPAGARDLIDAILAA
                     VRDFTGDTPQADDLTIVTVRRTNA"
     gene            2484..3119
                     /locus_tag="PK829_01580"
     CDS             2484..3119
                     /locus_tag="PK829_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU39934.1"
                     /translation="MSIRVLLVDDHSVVIDGLRMFLGLDPELEIVGQAADGAEAVALA
                     HRLRPDVVLMDLLMPTMDGITATGLIRRELPEVEVVALTSVLEDEKVIGAIRAGAIGY
                     LLKDTEADELRRAIKAAAAGQVQLSPQVVERLLHEVKEPEDPARLGDYEEEVLRLLAR
                     GRSNDEIARALHVQEKTVKLLIGDILAKLGVPSRTQAALYAVRTGLVTLEE"
     gene            3277..3804
                     /locus_tag="PK829_01585"
     CDS             3277..3804
                     /locus_tag="PK829_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39935.1"
                     /translation="MITKESFTVEEWAQIISAPASIGALVVTADPSGPMGLIGEFRAI
                     MSSMQAYVEAHAAESGLMGALKGYMSSQPTEQEQEQLKQWASAQQEEMKANRPNTPEE
                     LQQRVREAVTNVMALLRGKGATEEEVSGFKAMMVSIAEAVAEASKEGGFLGFGGVRVS
                     DKEQSILTQIRAELS"
     gene            3811..4749
                     /locus_tag="PK829_01590"
     CDS             3811..4749
                     /locus_tag="PK829_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU39936.1"
                     /translation="MAGGKDAAARRHRAGGQAFRAGDGRVGWLLLPYAAGLVLLVLLP
                     ALLSFGLAFFRADGLSPPRFVGGLNFTLVFTDELYTLSVANSLALVILPAPVRVLGAF
                     LLAWLARRGRLSALLRAAVFLPSAVPASAFALAWLWVLNPLYGPLNRLLAGLGLPGPG
                     WLADGQWARPGILLLLLWLVGEGFLVALAALYDVPPELEDAAAVDGAGAAATLRHVLL
                     PLLAPVLALLAARDAVLLLPDAYVFARLLTGGGPYYATYTLPQFIHEAAFDRLDFGVA
                     AAAVWLLYALTGLLLVVVYLVARQWNVGTSESDFLV"
     gene            4759..5580
                     /locus_tag="PK829_01595"
     CDS             4759..5580
                     /locus_tag="PK829_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU39937.1"
                     /translation="MRLSPCSRLTLTLVRALAAAIFGLPLLWALLAALRPAGTPLAAP
                     LLAVRPTLDAFGRLFAVLPVWRFTANSLLVAAIAVPLTLLTASWAGFAMARRPRPAQR
                     RWVVVSLMALMVPGVALWPSRFLLYSRVGLIDTYGALLAPALMGSSPFYVLMFYRAFG
                     RIPSALYDAARLDGAGVLATWGRVALPIARPTAAAVAVLAFVATWGDYLSPLLYLSDT
                     RRYTLPVGLELLRQLGRADYPLLLAGALWATIAPLVVVVLTVLLLALGERRRAAH"
     gene            5710..6972
                     /locus_tag="PK829_01600"
     CDS             5710..6972
                     /locus_tag="PK829_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU39938.1"
                     /translation="MKRLWLLLPLLFLFACRSEPATVSFMVFGDAAEFAAYESLVKAF
                     ADAQDEVTVTLTHIPSQNDYRARLAADFAAGTPPDISLLNFRRVGAFAAADQLQPLGA
                     YLDDSDVIAPEDFYPITLAAFTWQGQLMCIPQNISSLVVYYNRDLFRAAGVAEPADDW
                     TWDDFLAAAQALTLDSDGDGQTDQYGLGVEASLYRLAPFIWQNGGELVDDPAFPTQLA
                     LPRVPALAALTWFTELQTVHGVVPGREAEASQDSESRFLAGTTAMYLNSRRGTPTYRE
                     SAAFDWDIAPLPHGAVEAGVLHSDGYCLSATAAGKDAAWRFIEFANSPEGQRIVAQTG
                     RTVPSLIAVAESDAFLDPAQRPARSRVFLDTIPTLRLVPVLSTWNDIESTASEEIERA
                     FYGDISPEEAAILARDRTDEYFQLAVRP"
     gene            6969..>7902
                     /locus_tag="PK829_01605"
     CDS             6969..>7902
                     /locus_tag="PK829_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39939.1"
                     /translation="MSRRDPLAETLAHLSAARANPHAPGAAETLRRALADRSNLVVAR
                     AAEIVAEWELAEAAPDLMAAFDRFMIDPARRDPTCAAKIALAEALLRLDAGDAEHFRR
                     GLGHIQPEPVWGGTQDTAAPLRAICAVGLARANPPDVLLDLAGLLADPEADARAGAAR
                     AIAAAGRPGGAALLWFKVRAGDVEVGPLAECFAGLLALEPERAVAFVGGYLEADSAAL
                     AEAAALALGRSGLAAAAPLLRDAWQTAGDPGRRQPFLRALALLGDEAAFAFLLELVAS
                     GPAGDARDALAALAVIDDRRLRRKIDRAAAGLGAR"
     assembly_gap    7903..8002
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8003..8356)
                     /locus_tag="PK829_01610"
     CDS             complement(<8003..8356)
                     /locus_tag="PK829_01610"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOU39940.1"
                     /translation="MLLLVACGGGESAGPTNVYIADENDNGQTVTMGVGDALHLTLPE
                     NRSTGYVWSVVTNDESILRPDAEPTYEIEGEAMPGAGGQVTFNFIAAREGRVNLQLIL
                     ARPQETAVEAAQTFAL"
     gene            complement(8486..11419)
                     /locus_tag="PK829_01615"
     CDS             complement(8486..11419)
                     /locus_tag="PK829_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOU39941.1"
                     /translation="MITAEQLADFAAALRPRIAGELRTDAMTRVLYSTDASLYQVMPH
                     AVLIPRTTEDVIAAVSVAAAHDVPVLPRAAGTSLAGQAVNAALVIDFTRHLDAILAID
                     PAARRATVQPGVVVDDLNAALRPHGLQFGPDPASSDRSTLGGAVANNATGSHSARYGM
                     TADHVTAMDVVLADGATARLTAGDGGGTAPSGAAAIRAAVAALLADAGNQRAIDAGTP
                     RHWRRCGGYALDRLRPGPAADRAGDLAALVCGSEGTLAVMTAITLNLVPRPAHTGLVL
                     LHIDDLHAALAAVPDILTTDPSAVELLDTLSLALGDNVPEYARLLAAVVPGRPRGLLV
                     VEYSGESEAAVRAGATRLERLAAQLPGIVAATPALSPAHQAAVWAVRKANLGLLMSMK
                     GDMKPVPSIEDAAVPVADLPEYVRRVEEFCAGLDTPISYYAHAGAGCLHIRPLINRRS
                     AAQLARLPEIARFSAAAVRGYGGALSSEHGDGRARSWLNEEFYGPELYALFRRVKRIF
                     DPANRLNPGIIVDAAPMTASLRRLPELPGPARLDFSDYERDTPLPLVVAPRPEPAAVA
                     PGLVRAVELCNGAGVCRRRTGGTMCPSFMVTREEAHSTRGRANALRAVMSGVLPAAEL
                     TGPGMAAVMDLCISCKACRAECPSGVDMARLKTEYLARTYEAHGVPLRARLFAHAGAL
                     NRWGGGWRAPLAGALLNSRARRWLGLTTARPLPVPARRSFRDWWRDWWRDSPESRAPD
                     LALLIDPFTDAIQPAVGIAAVEFLRAAGLRPRPVVVDDGRACISKGLVGAARRAAART
                     VAALEWAVAAGLPVVGLEPSSLLTLRDEYLYLLPGDGRAAALAAAARTFEEFVAERAA
                     AGALPVRFAEGPWRMLLHGHCHQKALVGTGPARRALALLPGAVVEEVDSGCCGMAGSF
                     GYEAEHYAISMQMAERRLLPAVRAAGPETIIVAAGFSCREQIAHGSGRRALHPAEVLR
                     AALLP"
BASE COUNT         1648 a         4199 c         4020 g         1750 t
ORIGIN      
        1 caggacgacg aacaggccgc tgacggtcag cccgcggccg atggcctcga cccattgctc
       61 gccgggcagg atgtcgtccg gggcgatcca caccgggcgg ccagcctcgc gcagggccgt
      121 ggccagccgg tgggccagct cgacgtcttc ctcggcgtgg ctgatgaaga cgcggcgcgt
      181 caggcgggtg gtggaagccg acggcggcgc ggccaactgg ggcagcttct tgaaagcctc
      241 ggcgaactgc tccggccgca ccttgcccag agcgacgagc aagtgctcgc gttggccacg
      301 gcggtcggcg aaactgatca gcagccgcgc cttcctgcca accggcatcc ccggcgcgaa
      361 ctcctgcgac gccgctggaa agtagtcaaa gcaaaaatcc tccagctcat cgtcgctgaa
      421 ggcgttgatg aagaattgcc gcagttggtc gttcggaatg tccatctcag gagctgccga
      481 gagccgtgcg catatcatag ccgaagaggg aggcggacac aatgggtagt gacgcaggga
      541 ttttcccaaa gtcacccccg gcgcgctcac ctgtcaggtc aatggccgcg ccgggcggcc
      601 ggccatctct catgtcccgg cgcgcgttgt gctataatcg gccccaagtg acgccatgac
      661 cattcgtatc attattgccg atgaccacag cgttgtccgc caggggctgc ggatgtttct
      721 gggcctcgac cccgagttgg aagtcgtcgg cgaggcggtc aatggcgcgg aggccgtcca
      781 gaaggccgaa gccttgcagc ccgacgtggt gctgatggac ttgattatgc cggtcatgtc
      841 cggactagag gccacggccg aaatccgccg ccgcggcctg gccgcgcagg tcatcgtcgt
      901 caccagcgtc atcgaagagg ccgcagtcac ggccgtcatt cgcgccgggg ccatcggcta
      961 cttgctgaaa gataccgaag ccgacgaact gcgccgggcc atcaaggccg ccgccgccgg
     1021 ccgcgcccag ctttcccccg ccatcgccgc ccgtctgctg gccgagacac agagcagcga
     1081 cgccgacacc ctgaccgacc gcgagaatga ggtcttgcgc ctggccgccg ccggcctgtc
     1141 agaccaggag atcgctctgc agctgcttgt ctcggagccg gtcgtccact cccacgtcgc
     1201 tggcatcgtc gagaagctgc ggctggccaa ccagtcgcag acgctgctgc tcgacttcgc
     1261 caaccgcgcc cagacctatc tggacccgac ggcgctgctc gacttcctgg tgaccgaggt
     1321 cccgcgcctg ctgcaagccg acgccgccgc gctgctgttg cccgaacccg gcgatgggcg
     1381 actggcgttg cgcgccgccc acaactggcc cgccggcccg ctgaacgaaa cgctgggcgc
     1441 gggggcgctg agcgccttcg agagccgcca gccgtggcag gacggccgcg acgacgcggc
     1501 cggccctctg gccagcgtcg ccttcatccc tctactcatc ggcggcggag ctatcggcgt
     1561 tctgggactg ggcggccaga cgccgcgccg cctcaacgag gacgagttgc gctttgcccg
     1621 cctgctggcc aaccaggcag ccctcagcgt cgagcaggcc cgcctccacc gcgtcgagag
     1681 ccgcggccag cgcatggagc gcgaacttca gcaggcgcgc cagattcaga tgagcctgtt
     1741 gcccagcgag tacccacagg tggccggcta cgacttcgcc gcggcctatg agtcggcgcg
     1801 cgagatcggc ggcgatctct atgacttcat cgagtggccc ggcgaccccg accgcatcgg
     1861 cctgctcatc gccgacgtca gcggcaaggg cacggccgcg gccctcttca tggcccatag
     1921 ccgggcgatg atccgcggcg cggcccagac ccagcccggc ccggcggcga cgctggcggc
     1981 ggccaacgtc cagatcgccc gcgacaacca cgccatgctc tttgtcagcg ctttctatgc
     2041 cgtcctcgac gcgcccagcg gccggctgac cttcgccaac gccggccaca acgtgccgct
     2101 ggtgcgccgc gccaacggcg gcgttgaaga agctctcgcc cgcggcatgg tgctgggcat
     2161 catggacgac atgacttatg atgaagcgac gacaactctg gccgggggtg atatcatcgt
     2221 gctctatacc gacggcatca ccgaagcaat gaacgccagt ggtgagttgt tcgacaagga
     2281 gcggctgatg gccgccattc gcggggccaa cccggctggt gcgcgcgacc tcattgatgc
     2341 catcctcgcc gccgtgcgcg acttcaccgg cgacacgccg caggccgacg acctgaccat
     2401 cgtcacggtg cgcagaacga acgcgtgaag gtgctgcctg cccttccgtc cggcggccgc
     2461 agccgcccgc aagttggatg cttatgtcaa ttcgagtcct gctcgtagat gaccacagcg
     2521 tcgtgatcga cggcctgcgg atgttcctgg gccttgaccc ggaactggag atcgtcggcc
     2581 aggcggccga cggggccgag gccgtggcgc tggcccaccg gctgcggccg gatgtcgtcc
     2641 tcatggacct gctgatgccg acgatggacg gcattaccgc cacgggcctg atccgccgcg
     2701 aactgccgga ggtcgaggtg gtcgccctga ccagcgtcct ggaggacgag aaggtcatcg
     2761 gggccattcg cgccggggct atcggctatc tgctgaaaga caccgaagcc gacgaattgc
     2821 gccgggccat caaggccgcc gccgccgggc aggtgcaact gtcgccccag gtcgtcgagc
     2881 ggttgctaca cgaggtcaag gagccggagg acccggcccg cctgggcgac tacgaggagg
     2941 aggtgctgcg gctattggcc cgcgggcgct ccaacgacga gatcgcccgc gccctccacg
     3001 tccaggagaa gacagtcaag ttgctcatcg gcgatatact ggccaagctc ggtgtaccca
     3061 gccgcaccca ggccgccctc tacgccgtcc gtacgggact ggtgacgctg gaagagtagg
     3121 aaggaggagg gatgaaggag gaaggaggaa ggaggaagga tgacagacca cagactgacc
     3181 tactgtccac tgacctactg acccactgac ctactgaccc actgaccact gtctactgtc
     3241 cactgtccac tgtctactgt cgaaaggagt acgcacatga tcaccaagga atcgtttacc
     3301 gttgaagaat gggcgcaaat tatctctgcg ccggcgtcga ttggcgcgct ggtcgtcacc
     3361 gccgatccgt ccgggccgat gggcctgatt ggcgagttcc gcgccatcat gagcagcatg
     3421 caggcctacg tcgaggccca tgcggctgag agcgggctga tgggcgcgct gaagggctac
     3481 atgagcagcc agcccaccga gcaggagcag gagcagctca agcagtgggc atcggcgcag
     3541 caggaggaga tgaaggccaa ccggcccaac acgcccgaag aactgcaaca gcgcgtccgc
     3601 gaggcggtca ccaacgtcat ggccctgttg cgcggcaagg gggccacgga agaggaagtc
     3661 agtggcttca aggccatgat ggtcagcatc gccgaggccg tggccgaggc cagcaaggag
     3721 ggcggcttcc tgggcttcgg cggcgtgcgc gtctccgaca aggagcagtc gatcctgacc
     3781 cagatccgcg cggaactgag ctaaccggct gtggccggcg gcaaggacgc ggcagcccgg
     3841 cggcacaggg ccggcggcca ggcatttcgt gccggcgacg ggcgcgtagg ctggctgctg
     3901 ctgccctatg ccgccggcct cgtgctgctc gtgctcttgc cggcgctcct gtcctttggc
     3961 ctggccttct ttcgcgccga cggcctcagc ccgccgcgtt ttgtcggcgg gctgaacttc
     4021 accctggtct tcacggacga gctatatacc ctcagcgtgg ccaactcgct ggcgctggtc
     4081 atcctgcccg cgcctgtgcg cgtgctgggc gcgtttttgc tggcctggct ggcgcggcgt
     4141 ggccggctga gcgccctcct gcgcgcggcc gtcttcctgc ccagcgccgt gcccgcctcg
     4201 gcctttgcgc tggcctggct gtgggtgctc aatccgctct acgggccgct caaccggctg
     4261 ctggccggcc tgggcttgcc ggggccgggc tggctggccg atgggcaatg ggcgcggccg
     4321 ggcatcctgc ttctgctgtt gtggctggtg ggcgagggct tcctggtggc cctggcggcg
     4381 ctctatgacg tgccgcctga gctggaagac gccgcggcgg tggacggcgc gggcgcggcg
     4441 gcgacgctgc gccacgtgct gctgccgctg ctggcccccg tgctggcgct gctggccgcg
     4501 cgggacgccg tgctgttgct gcccgacgcc tacgtcttcg cccgcctgct gaccggcggc
     4561 ggcccctact acgctaccta caccctgccg cagttcatcc acgaggcggc cttcgaccgg
     4621 ctcgacttcg gcgtggccgc ggcggcggtg tggctgctct atgccctcac cgggttgctg
     4681 ctggtcgtcg tctatctggt cgcccggcag tggaacgtgg gcaccagcga gagcgatttc
     4741 ctggtctagg taacgaccat gcgcctgtcg ccctgctccc ggctcaccct gacgctcgtg
     4801 cgcgctctgg cggcggcgat cttcggcttg ccgctgctct gggcgctgct ggcggcgctg
     4861 cggccggcgg gcacgcccct ggccgcgccg ctgctggccg tccggccaac gctcgacgcc
     4921 ttcggccgcc tcttcgccgt cctgccggtg tggcgcttca cggccaactc gctcctcgtc
     4981 gccgccatcg ccgtgcccct gaccctgctg acggcctcgt gggccggctt cgccatggcc
     5041 cggcggccgc gaccggcgca gcgccgctgg gtggtcgtct cgctgatggc cctcatggtt
     5101 cccggcgtgg ccctgtggcc gtcgcgcttc ctgctctaca gtcgggttgg cctgatcgac
     5161 acctatggcg cgttgctggc cccggcgctg atgggcagca gcccgttcta tgtgctgatg
     5221 ttctaccgcg ccttcggccg catcccctcg gccctctacg atgccgcccg gctcgacggc
     5281 gcgggcgtgc tggccacgtg ggggcgcgtg gccctgccca ttgcccggcc gacggccgcc
     5341 gccgtggccg tgctggcctt cgtcgccacg tggggcgact acctgtcgcc actgctctac
     5401 ctgagcgaca cgcgccgcta cacgttgccc gtcgggctgg agctgttgcg gcaactcggc
     5461 cgggccgact acccgctgct gctggccggg gcgctgtggg ctacgattgc gccgctggtt
     5521 gtagtggtgc tgaccgtctt gctgctggcg ctgggcgagc gccggcgggc cgcccactag
     5581 cgcccggccc ttcccgcgcc caatgcctgg cactttctcg cccagccccc caagaaggtg
     5641 cgacgcactc cagaaagtgc gacgcaccgc gtaccgcgac gggcgtcttg aatgcgttat
     5701 actacgacca tgaaacggct ctggctcctc ctcccccttc ttttcctatt cgcctgccgc
     5761 agcgaaccgg caaccgtgtc ctttatggtc ttcggcgacg cggccgagtt cgccgcctac
     5821 gagtcgcttg tcaaagcctt cgccgacgcg caggatgagg tcacggtcac gctgacccac
     5881 atcccgtcgc aaaacgacta tcgcgcccgc ctggcggccg atttcgccgc cggcacgccg
     5941 cccgacattt cgctgctcaa cttccgccgt gtgggcgcgt tcgccgccgc cgaccagctc
     6001 cagccgcttg gcgcttacct ggacgacagc gacgtcatcg ccccggagga cttctacccc
     6061 atcaccctgg cggccttcac ctggcagggc caactgatgt gcatcccgca gaacatctcc
     6121 agcctcgtcg tctactacaa ccgcgacctc ttccgcgccg cgggcgtggc cgagccggcc
     6181 gatgactgga cgtgggacga cttcctggcc gcggcccagg ccctgacgct ggacagtgac
     6241 ggcgacggcc agaccgacca gtacggcctg ggcgtggagg catcgctcta ccggttggcc
     6301 ccattcatct ggcagaacgg cggcgagctg gtcgatgacc ccgcctttcc cacgcaactg
     6361 gccctgccgc gcgtgcccgc gctggcggcc ctgacgtggt tcacggagtt gcaaactgtc
     6421 cacggcgtcg tgccgggccg cgaggccgaa gcctcgcagg acagtgagag ccgcttcctg
     6481 gccggcacga cggcgatgta cctcaacagc cgccgcggca cgccgaccta ccgcgagtcg
     6541 gccgccttcg actgggacat cgccccactg ccccacggcg cggtcgaggc cggcgtgctc
     6601 cacagcgacg gctattgcct gtcggccacc gcggccggca aggacgccgc ctggcgcttc
     6661 atcgaattcg ccaactcgcc ggaggggcag cgcatcgtcg cccagaccgg ccgcaccgtg
     6721 ccctcgctga tagccgtggc cgagtccgac gctttcctcg acccggccca gcgcccggcc
     6781 cgttcgcgcg tcttcctgga taccatcccc acgctgcgcc tcgtgcccgt gctaagcacc
     6841 tggaacgaca tcgagagcac cgcgtcggag gagatcgagc gcgctttcta cggcgacatc
     6901 agcccggagg aggcggccat cctggcgcgc gaccggacgg atgagtactt ccaactggcc
     6961 gttcgcccat gagccggcgc gatccgctgg ccgagacgct ggcccacctg agcgccgcgc
     7021 gggccaaccc ccacgcaccc ggcgcggccg agacgctgcg ccgggcgctg gccgaccgct
     7081 ccaacctcgt cgtcgcccgc gcggccgaga tcgtcgccga gtgggaattg gccgaagccg
     7141 cgcccgacct gatggccgcc ttcgaccgct ttatgatcga cccggcgcgg cgcgacccca
     7201 cctgcgcggc caagatcgcc ctggccgagg cgctgctgcg gctcgacgcc ggcgacgcgg
     7261 agcacttccg ccgcggcctg ggccacatcc agccggaacc ggtctggggc ggcacgcagg
     7321 acacggccgc gccgctgcgg gcgatctgcg cggtggggct ggcgcgggcc aacccgcccg
     7381 acgtgctgct cgatctggcc gggctgctgg ccgacccgga agccgacgcc cgcgccggcg
     7441 cggcgcgggc catcgctgcc gccggccggc cgggcggcgc ggcgctgctg tggttcaagg
     7501 tacgcgcggg cgacgtcgag gtggggccac tggccgagtg ctttgccggg ctgctggcgc
     7561 tggagccgga gcgcgccgtg gcctttgtcg gcggctatct ggaggccgac agcgcggccc
     7621 tggccgaggc ggcggcgctg gccctgggcc ggtcagggct ggccgcggcc gcgcccctct
     7681 tgcgcgacgc ctggcagacg gccggcgacc ccggccggcg gcagccgttc ctgcgggcgc
     7741 tggccctgtt gggcgacgag gccgctttcg ccttcctgct ggaactggtg gccagcggcc
     7801 cggccggtga cgcccgcgat gccctggcgg ccctggccgt catcgacgac cgccgcctgc
     7861 gccgcaaaat cgaccgcgcc gccgccggac taggggctag ggnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nncagggcga acgtctgcgc cgcctccacg gccgtctcct
     8041 gcggccgggc gaggatgagt tgcagattga cgcgcccctc gcgcgcggcg atgaagttga
     8101 acgtcacctg cccgcccgcg ccgggcatgg cctcgccctc gatctcgtag gtcggctcgg
     8161 cgtccggccg caggatactc tcgtcgttgg tgacgacgct ccagacgtag ccggtgctgc
     8221 ggttctccgg cagggtcagg tgcagcgcgt cgcccacgcc catcgtcacc gtctggccgt
     8281 tgtcgttctc gtcggcgatg tagacgttgg tcgggccggc gctctcgccg ccgccacagg
     8341 cgaccagcag cagcatgacc actagaagtg gcgcgattct cctgaatcgc gttcttgggc
     8401 cggcgcggaa caaaaagcga ttcgggagaa tcgcgccaca cggttgcctg gctatacgaa
     8461 agtccatctc cctttccctc caactttaag gtagcaacgc cgcccgcaac acctcggccg
     8521 ggtgcagcgc ccgccgcccg ctgccgtggg cgatctgctc ccggcagcta aagccggcgg
     8581 cgacgatgat cgtctccggc ccggcggcgc gcaccgccgg cagcagccgc cgctcggcca
     8641 tctgcatgga aatggcgtag tgttcggcct cgtagccgaa cgagccggcc atgccgcagc
     8701 agccggaatc gacctcctcg acgaccgcgc cgggcagcaa ggccagcgcc cgccgcgccg
     8761 gccccgtgcc caccagcgcc ttctggtgac aatggccgtg cagcagcatc cgccacggcc
     8821 cctcggcaaa gcgcaccggc agcgccccgg ccgccgcccg ctcagcgacg aactcctcga
     8881 acgtccgcgc cgccgcggcc agcgccgccg cccgcccgtc gcccggcaac aggtagaggt
     8941 actcgtcgcg cagcgtcagc aggctgctcg gctccagacc gaccacgggc agccccgcgg
     9001 ccacagccca ttccagcgcc gccaccgttc gcgccgccgc ccgccgcgcc gcgcccacca
     9061 gccccttgga gatgcacgcc cggccgtcat cgacgacgac cggccgcggc cgcaacccgg
     9121 ccgcgcgcag gaactccacc gcggcgatgc ccaccgccgg ctggatggcg tcggtgaacg
     9181 ggtcgatgag cagggccagg tcgggggcgc gcgattcggg agaatcgcgc caccagtcgc
     9241 gccaccagtc gcggaaggag cggcgggcgg gcacgggcag cggccgcgcc gtcgtcaggc
     9301 ccagccagcg ccgcgcccga ctgttcagca gcgccccggc cagcggcgcg cgccaccccc
     9361 cgccccaccg attgagcgcc ccggcgtggg cgaacagccg cgcccgcagg ggcacgccgt
     9421 gggcctcgta ggtccgcgcc aggtactccg tcttcagccg ggccatgtcc acgcccgacg
     9481 ggcactcggc tcggcaggcc ttgcaggaga tgcacaaatc catgaccgcg gccatgcccg
     9541 ggccggtcag ttcggccgcg ggcagcacgc cgctcatgac cgcgcgcaga gcgttggccc
     9601 ggccgcgcgt gctgtgggcc tcctcgcgcg tcaccatgaa gctcgggcac atcgtcccgc
     9661 ccgtccgccg gcggcagacg cccgcgccgt tgcacagctc cacggcgcgg acgaggccgg
     9721 gcgcgacggc ggctggctcc ggccgcgggg cgaccactag cggcaggggc gtgtcgcgct
     9781 cgtagtcgct gaagtccagc cgcgcggggc cgggcagttc tggcaggcgg cgcaggctgg
     9841 cggtcatggg cgcggcgtcg acgatgatgc cggggttgag ccggttggcg gggtcgaaga
     9901 tgcgcttgac ccggcgaaac agcgcgtaca gctccgggcc atagaactct tcgttcagcc
     9961 agctgcgcgc ccggccgtcg ccgtgctcgc tcgacagcgc cccgccatag ccgcgcacgg
    10021 ccgcggccga gaagcgggcg atctccggca ggcgagccag ttgcgccgcg ctgcgccggt
    10081 tgatgagcgg ccggatgtgg aggcagcccg cgccagcgtg ggcatagtag ctgatgggcg
    10141 tgtccagccc ggcgcagaat tcctccaccc ggcgaacgta ctccggcaga tcggccacgg
    10201 gcacggcggc gtcctcgatc gacgggacgg gcttcatgtc acccttcatc gacatcagca
    10261 agcccagatt ggccttgcgg acggcccaga cggcggcctg atgggcgggc gacagcgccg
    10321 gcgtggccgc gacgatgccg ggcagctgcg ccgccagtcg ctccagtcgc gtcgccccgg
    10381 cccgcacggc ggcctcgctc tcgccgctgt actcgacgac cagcagcccg cgcggccgcc
    10441 ccggcacgac cgcggccaac aggcgggcgt actccggcac gttgtcgccc agggccaggc
    10501 tgagcgtgtc cagcagttcg acggccgacg ggtcggtggt caggatgtcg ggcacggccg
    10561 ccagcgcggc gtggaggtca tcgatgtgga gcaacaccag gccagtgtga gccgggcggg
    10621 ggacaaggtt gagggtgatc gcggtcatga ccgccagcgt gccctcggag ccgcagacga
    10681 gcgcggccag atcgccggcc cggtcggcgg ccgggccggg ccgcagccgg tcgagggcgt
    10741 agccgccgca gcgccgccag tggcgcggcg tgcccgcgtc gatggctcgc tggttgccgg
    10801 cgtcggccag cagggcggcc acggcggcgc ggatggccgc cgcgccggat ggggccgtgc
    10861 caccgccgtc gccggccgtc aggcgcgccg tagccccatc ggccaggacg acgtccatcg
    10921 ccgtcacgtg gtcggcggtc atgccgtagc gggccgagtg gctgccggtg gcgttgttgg
    10981 ccaccgcgcc gccgagcgtc gagcggtcgc tgctggccgg gtcggggccg aactggaggc
    11041 catgcggccg caaggccgcg ttgaggtcat cgacgacaac gccgggctgc acggttgccc
    11101 gccgcgcggc cgggtcgatg gccaggatgg cgtcgaggtg gcgggtgaag tcgatgacca
    11161 gggcggcgtt gacggcctgc ccggccaggc tggtgcccgc cgcccgcggc agcacaggca
    11221 cgtcgtgggc cgcggcgaca ctcaccgccg cgatgacgtc ctcggtcgtc cgcgggatga
    11281 gcacggcgtg gggcatgacc tggtagaggc tggcgtcggt gctatagagc acccgcgtca
    11341 tggcgtcggt gcgcaattcg ccggcgatac gcggccgcag cgccgcggcg aagtcggcca
    11401 gttgctccgc ggtaatcatg gagcgatctt agtgcgtcgc ggcggggagg caagtggatg
    11461 gcgggaaggg gctagggatt aggggctagg ggttaggggt taggggtcag ggaagagcgc
    11521 tcttcccctg gcccctggaa cctggcccct ggaacctggc ccctggaacc tggcccctgg
    11581 ccccttcccc cgacttttta tcaattaact ctgcttattg acaaattgtt accaatgagt
    11641 atacttatga atggctcccc ccccagaggt ggacagtagg acgcccgtct tactgccacc
    11701 tcatttttta tggatcg
//
LOCUS       DAOSMQ010000025        11659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5491_length_11659_cov_4.849104, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000025 DAOSMQ010000000
VERSION     DAOSMQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5491_length_11659_cov_4.849104"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PK829_01620"
     CDS             complement(<1..372)
                     /locus_tag="PK829_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOU39942.1"
                     /translation="MNQSDFKRISPYLYEIPRSYRSDMRAPARFYADAELLRQALSDK
                     SLDQLVNSATLPGIVAYALAMPDIHQGYGFPIGGVIATETPDGVISPGGVGYDINCGV
                     RLRRRDGPGAHRRERPHCRGRP"
     gene            complement(408..836)
                     /locus_tag="PK829_01625"
     CDS             complement(408..836)
                     /locus_tag="PK829_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOU39943.1"
                     /translation="MTNPEPPDDDIQIIDHTADWALRVRAADLPGLFHRAAVGMARLL
                     AGDLAAVPLDETRTLAVTADDAEGLLVEWLGELAYWAERDGFVAPQIEWDALSPVSLA
                     ATVRGGRLAELQKHIKAVTYHDLAIRPAGDGLEVTIVFDV"
     gene            complement(856..1671)
                     /locus_tag="PK829_01630"
     CDS             complement(856..1671)
                     /locus_tag="PK829_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU39944.1"
                     /translation="MSAMCPNCGKPVLPTDTACWHCGYALPKRPKTPLPARPRPKDDA
                     PAEAATADYDLRALVVYGLLTLAVVLSLWLVMRALGQQPVLVRSAALAGGDWVSVTDA
                     DLRYTLSLPGGWQWLDLSYRDQQALLAEVIDRQGYVAHALDPLARPAADVETLAVAAN
                     TRDLNATEPIPFVVVGRSERLRDLSPEDALALLAGHDRPVTETAVDRHLAGQPQARFT
                     VSDFANSYQCRHLFVAGLDAAGYLVAACAPPAEFGTLGRDLNAILDSFQLLQR"
     gene            1878..2474
                     /locus_tag="PK829_01635"
     CDS             1878..2474
                     /locus_tag="PK829_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU39945.1"
                     /translation="MTDPLELLLSRRSIRRYTDRPLSPETIDHLLTAAVWAPSAHNRQ
                     PWRFVVMTGATRARLAAAMGAQLRRDLAADGLPAEAIARDADRAYARISSAPALVLLC
                     LTLADMDAYPDARRSAHETTMAVQSVAMAGQNLLLAAHALGLGACWLCAPLFCAAVVR
                     QTLDLPADWQPQGLITLGHPAEERQKTRAPLETRVLYR"
     gene            complement(2548..4194)
                     /locus_tag="PK829_01640"
     CDS             complement(2548..4194)
                     /locus_tag="PK829_01640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOU39946.1"
                     /translation="MTSVLRILPLGGLGEIGKNMTAFEYEDDILIVDAGVMFPENDML
                     GVDLIIPDYHYLMEEDRLSKIRAIIITHGHEDHIGALPHIIEDIRAPIYATPLTLGLI
                     EAKLREARRLDDVELVSFRAGEVLEIGPFKIEPFHVAHSIPDCVGFGITTPVGLIVHT
                     GDYKFDHTPIDNWPPDFAKLAEFSQRGVLALMADSTNADRPGWTESEMDLAQGFEDVF
                     RTAPGRIIVATFASLISRVQIAAAAAQKFNRKIAIAGRSMRDAVKIARKLGYLEVPDD
                     LIIDISQAVTLPPSRVVIMATGSQGEPSAVLGRLARGQHKQLSIQEGDTVIFSAHPIP
                     GNEELIYRTINQLYRRGATVLYDRIAKVHVSGHASREEMKLLINLIKPVFLIPIHGEL
                     RHLKLHAQIGQTLGIPAERIAVVENGTPIDLTPYSMTVRERLPGGYVFVDGSGVGDIG
                     WAEVRDRDRLAQAGYFFAAVPTDGRGGMAGPPQLSTRGFVNEAEAGELLLEAEKLIAR
                     TAAEAQREGRPISASQIEGALNRYLYDETRKRPVIEVVVV"
     gene            complement(4371..5363)
                     /locus_tag="PK829_01645"
     CDS             complement(4371..5363)
                     /locus_tag="PK829_01645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOU39947.1"
                     /translation="MAETISLSAAWRTDTGLVRGHNEDFVKVYEPATEDERRRHGAVY
                     IVADGVGGADAGEVASQYASERTLHHYLAADGRTDWGQRLIDAMQAANTDLRRLAAGR
                     DDSRRMATTMVAVVIQDGQAFIGNVGDSRAYQWRKGRLEQISRDQSLVARLVEEGALT
                     PEEAVHYPYKNVILYSIGSEKRPPIDLYPIVLEPGDLLVLCSDGLTRHVEDGEIAAIA
                     GDERPDVAADILVRLARERGGEDNITVAIIQHGERLEESTQPTARLTAAQPVVDAPAA
                     SIPVPVVAPPPVPQGGGPPRERPRSLWPLTFALVAIMAVLIFVLWVVVQRATTI"
     gene            5529..6710
                     /locus_tag="PK829_01650"
     CDS             5529..6710
                     /locus_tag="PK829_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOU39948.1"
                     /translation="MPADNRRPHSETRTIRTRARASHYREQVTPIYMASGFTFDSADQ
                     ARAVYGGEEPGYVYTRWDNPNSAELVARLCGMEGAEAGLPMASGMAALFTILAGLLNS
                     GDHVVASRSLFGATHQLLTTILPRWGIAHSYADCELTETWPGLFRPETRLCLVETPSN
                     PDLTLVDLAWVAALCRERKVLLVVDNTFATPVVQQPLALGADLVVHSTTKFLDGQGRT
                     IGGVILGDRLTVNELTHFARQTGPTLSPFNSWLLSQSLETLSLRMERHCANALALAEW
                     LSRRAGVETVRYPFLPSHPQAELARRQMSAGGGIVTFEVSGGLPAGRRFLDGLKMCDI
                     VANLGDARTTVTHPASTTHSALTDGERRVVGITPGLIRVSVGLEHIDDIIADVDQALG
                     N"
     gene            complement(6707..6922)
                     /locus_tag="PK829_01655"
     CDS             complement(6707..6922)
                     /locus_tag="PK829_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39949.1"
                     /translation="MLRRPLRRACALPPDAPPALAESDPTEAFYAIDAAHMLAIVAPP
                     AEALAWNRRAPTNWLLPLTGRAFTPNS"
     gene            complement(7124..10258)
                     /locus_tag="PK829_01660"
     CDS             complement(7124..10258)
                     /locus_tag="PK829_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU39950.1"
                     /translation="MSRKNTLKLVVIVGLLLITASIAFAQDATQPSRAETGRTIPDQV
                     KTLRLDTPIQVSGAEAAFRLAPELRNATGRQQVVVRLSQPALAVAPAAEGAMAAAVSQ
                     QAEVVAAARSLDSNVRVLATLRMALNAVVIEADSATLAQLARNPNVVAITPLRNYELD
                     LSETVPYIGATAVQNEGYDGSGVRVGVIDSGIDYTHAVFGGPGTVEAYENNDPTIIEP
                     GTFPTERVVGGYDFVGSEWPNGDIMPDPDPLDDGPGGGHGTHVADIIGGNLGVAPGVD
                     LYALKACSSVSTSCAGLPLLQAIEFAVDPNNDGDTSDHLDVINMSLGSPYGDPRWDDL
                     SYAVDNASAAGVLTVASAGNSADKPYAHGTPAAAVTALAVAQTNVPSATQGLMEVVEP
                     ESIAGLYAAVHQPWSPEVTDPIQAPLQYGDGSGGNLDGCTAFPAGSLTGKIVLVDRGT
                     CAFSIKISNIAAGGAMAGIIGLITPGEPFAGSYGGGDPSVPGFMISQADAQLLKDALE
                     EGQVIVAFDPANDIPLVGHMVGSSSRGPSGGLNMAKPDIGAPGASVSAIHGTGTGTEA
                     FSGTSGAAPMVTGSAALLMDAYPDRSWAEIKSLLMNTAETDIMNTPEFFGGYLAAISR
                     IGGGEVRVDRALHSPIAAWDRDDLTGSLSFGFHEVTGQLDMLKRVTIRNYTDSPVTLR
                     SEVDYRYDNDANGEVRVTVPTLITVPANGTVGVPVRMFIRPRLSRPLHEWVLNSGSQG
                     ANPDALTLVEYDGYIRFIGVGANGQGADAHVAWHVLPRAAGRPETGFVPQTNTGWVRN
                     TGVSPMYIDTYSLLGSSPQIAGDPPLGGQTAPVDLRWVGVQTYPVPAGVCGPVDSFVM
                     GLAVNTWDRYSHASHVLVGFDLDLDGDGTFDYEVFNYDASGAFGSAPSDGRSLTWVSN
                     LATGAASASFYTQHETNSGNFVLFFCGQQIGMNASNFFNTSMDASAFAFDYYYSGSID
                     TIEGMNIVPLGERYLTVFQNGDIGFTWLPARSPRTRFGIIDFGDQLNMTETGVLWLYG
                     PGSPGEARVWELQP"
     gene            10717..11304
                     /locus_tag="PK829_01665"
     CDS             10717..11304
                     /locus_tag="PK829_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOU39951.1"
                     /translation="MERLRRDFSTITLVMIPVAIAINIAIGQLIYTLKVPLYLDSIGT
                     IFVAAVAGPLAGMLTGALTNLVWGLSGINSFYTAFWITGAVIGLVAGLFSRFGWFKVW
                     WRAIIAGVITGLIAALISAPILTYVNGGVTGSGTDVITAVFRNAGLGILPSAFAQGVS
                     SDPLDKAISFFIVWGVIRALPLRFLDRFSRAEDIR"
     gene            11323..>11659
                     /locus_tag="PK829_01670"
     CDS             11323..>11659
                     /locus_tag="PK829_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOU39952.1"
                     /translation="MSTSFSLYIPRESGMHRLHPLTKLALVAFCLVLGLFLPGVWLTY
                     LAFMLLIVPLAAWAGVLPSFLRAVFKTALPVVISVFLVQGLFWPGGTPILHLGPLSLK
                     QEGLQFAIRS"
BASE COUNT         1950 a         3736 c         3944 g         2029 t
ORIGIN      
        1 gggtcggccc cggcaatgcg gccgttctct tcggtgcgct ccaggtccat ctctgcgcct
       61 gagccgcacg ccgcaattga tgtcatagcc cacgccgccg ggcgagataa ccccgtcggg
      121 cgtctccgta gcgatgacgc cgccgatggg gaagccgtag ccctggtgga tgtcgggcat
      181 ggccagcgcg taggcgacga tgccgggcaa cgtggcgctg ttgaccagtt gatcgaggct
      241 cttgtcgctc aatgcctgtc gcagcagttc agcatcggcg tagaagcgcg ccggggcgcg
      301 catgtccgag cgataggagc gcgggatctc gtagaggtaa ggtgaaatgc gcttgaagtc
      361 cgactgattc atgcagtttt cctcggccgt ccggcccgac cgggcggcta cacgtcgaag
      421 acgatggtca cttccaggcc atcgccagcg gggcgaatgg ccagatcgtg gtaggtgacg
      481 gccttaatgt gcttctgtag ctcggccagc cggccgccgc ggacggttgc cgccagcgac
      541 accggcgaca aggcgtccca ctcgatctgt ggggcgacga aaccgtcgcg ctcagcccag
      601 taggccagct cgcccagcca ctccaccagc agcccctcag catcgtcggc ggtcacagcc
      661 agcgtgcgcg tctcgtccag cggcacggcc gcgaggtcac cggccagcag ccgggccatg
      721 ccgacagcgg cgcggtggaa cagaccgggc aggtcagccg cgcggacgcg cagcgcccaa
      781 tcggcggtgt ggtcgatgat ctggatgtca tcgtccggcg gttcggggtt ggtcatgcgg
      841 cttattatag ccgaatcagc gctgcagaag ctggaagctg tcgagaatgg cattgaggtc
      901 gcgccccagc gtgccgaatt cggccggcgg cgcgcaggcg gccaccaggt agccggctgc
      961 atccaggcca gccacgaaga ggtgacggca ctgatagctg ttggcgaagt ccgacacagt
     1021 gaagcgcgct tgcggttgcc cggccaggtg gcggtcaact gctgtctccg tcactggccg
     1081 gtcgtgcccg gccagcaggg ccagggcgtc ctctggcgac aggtcgcgca gtcgctcgct
     1141 gcggccgacg acgacaaagg ggatcggctc ggtggcgttc agatcgcgcg tgttggcggc
     1201 cacggccagc gtttccacgt cggcagcggg gcgggcaagc gggtcgagcg catgggcgac
     1261 atagccctgc cggtcgatga cttcggccag cagcgcttgc tggtcgcggt agctcaggtc
     1321 aagccactgc cagccgccgg gcaggctgag cgtgtagcgc aaatccgcgt cggtgacgga
     1381 cacccagtcg ccgcccgcca gcgcggcgct gcgcacgaga acgggctgct gccccagggc
     1441 gcgcatgacc agccacagag acaggacgac ggccagcgtc agcaagccat agacgaccag
     1501 ggcgcgcaga tcatagtcgg ccgtagcggc ttcggccggc gcgtcatcct tcgggcgggg
     1561 gcgcgcgggc agcggggtct ttggccgctt gggcagggcg tagccgcagt gccagcaagc
     1621 ggtgtcggtg ggcagcacgg gcttgccaca gttgggacac atcgcgctca tcggccgtcc
     1681 ctgcgcccgc tggccgggcc aagtgtaaac cagtttacac tgcggctgta ttccgcttgc
     1741 gcgacaaaga gcgtcattgc ccgtatgata acgacatcat tcggcaacac aaatagtcct
     1801 cctttttctc ctcttcccgc aaaaagccct gctgtcccgg cagggcttct ttgcatctag
     1861 gggctacaat cgggcgcatg accgaccctc tggaactgct gctcagccgc cgctctatcc
     1921 gccgctacac cgaccggccc ctctcgccgg agaccatcga ccacctgctg acggctgccg
     1981 tctgggcacc gtcggcccat aatcggcagc cgtggcgctt cgtcgtcatg accggcgcga
     2041 ccagggcgcg gctggcggcg gccatgggtg cgcagttgcg gcgcgacctg gccgccgacg
     2101 ggctgccggc cgaggccatc gcccgcgacg ccgaccgggc ctacgcccgc atcagcagcg
     2161 cgccggcgct cgtcttgctc tgcttgaccc tggccgacat ggacgcctac cccgacgccc
     2221 gccgcagcgc ccacgagacg acgatggccg tccagagtgt ggccatggcc ggacagaacc
     2281 tgctgctggc ggcccacgcg ctggggctgg gggcgtgctg gctgtgcgcg ccgctcttct
     2341 gcgcggctgt cgtccgccag acgctcgacc tgccggccga ctggcagccg caaggcctga
     2401 tcaccctggg ccaccccgcc gaagagcgac aaaaaacgcg cgccccactg gagacgcgcg
     2461 tcctttatcg gtaagacagg ggctaggggc tagggatcag tggtctgtgg tcggcggtca
     2521 ctacgactga ccactgctca ctgtccacta taccacaacc acctcaatga ccggccgctt
     2581 gcgcgtctcg tcatagagat agcggttgag cgcgccctcg atctgcgacg cggagatggg
     2641 ccggccctcg cgctgggctt cggccgccgt gcgggcaatg agcttctcgg cctccagcag
     2701 cagctcgccc gcctcggcct cgttgacgaa gccgcgcgtg ctgagctggg gcgggccggc
     2761 cataccgccg cggccgtcgg taggcacggc ggcgaagaag tagcccgcct gcgccagccg
     2821 gtcgcggtcg cgcacctcgg cccagccgat gtcgcccacg ccggagccat cgacgaagac
     2881 gtagccgccg ggcaatcgct cgcggacggt catgctatag ggcgtcaggt cgatgggcgt
     2941 gccgttctcc actacggcga tgcgctcggc gggaatgccc aacgtctggc cgatctgggc
     3001 atgcagcttc aggtggcgca gttcgccgtg gatggggatc agaaagaccg gcttgataag
     3061 gttgatgagc agcttcatct cctcgcggct ggcgtggccg gagacatgca ccttggcgat
     3121 gcggtcatag agcactgtcg ccccacgccg gtagagttgg ttgatggtgc ggtagatgag
     3181 ttcctcgttg ccggggatgg gatgggccga gaagatgacc gtatcgcctt cctggatgct
     3241 gagttgcttg tgctggccgc gggccaggcg gccgagcacg gccgacggct cgccctggga
     3301 gccggtggcc atgatgacca cgcgactggg cggtagggtg acagcctggc tgatgtcgat
     3361 gatcaggtca tcgggcactt ccagatagcc gagcttgcgg gcgatcttga ccgcgtcgcg
     3421 catggagcgg ccggcgatgg cgatcttgcg gttgaacttc tgggccgcgg cggcggcgat
     3481 ctgcacgcgc gagatgagcg aggcaaaggt agcgacaatg atccggccgg gcgcggtgcg
     3541 gaaaacatcc tcgaagccct gagccaggtc catctccgac tcggtccagc cgggccggtc
     3601 ggcgttggtg ctgtcggcca tcagcgccag gacgccgcgc tgcgagaact cggccagctt
     3661 ggcgaagtcg ggcggccagt tgtcaatggg cgtgtggtcg aacttgtagt cgccggtatg
     3721 gacgatcaac cccaccggcg tcgtaatgcc aaagccgacg caatcgggga tgctgtgggc
     3781 gacgtggaac ggctcgatct tgaacgggcc gatctccagc acctcgccgg cgcggaagga
     3841 aaccagttca acgtcgtcca gccggcgggc ctcgcgcagc ttggcctcga tgaggcccag
     3901 tgtcagcggc gtggcgtaaa tgggcgcgcg aatgtcctcg atgatgtgcg gcagcgcgcc
     3961 gatatggtct tcgtggccat gcgtaatgat gatggcgcga attttggaga ggcgatcctc
     4021 ttccatcaag taatgataat cgggaatgat gagatcgacg cctagcatgt cattttcggg
     4081 gaacatgacg cccgcatcga cgatgaggat gtcatcctca tactcaaaag cggtcatgtt
     4141 tttgccgatc tcacctagcc cccccagggg tagaatacgt aatacacttg tcatattgat
     4201 ccgctattat atgagatttt ggccggcgcg tcaaagttac tcagaggcgc atgagggcgg
     4261 gaaagcaggg acgacaacga gcttcaagcg aggctgcaag gaggaggaca gctctggcgt
     4321 ctatgcgttg tgttctcttg cgggcagagc gctagatggt cgtggccctg ctagatagtc
     4381 gtggcccgct ggacgacgac ccacaggacg aagatgagca cggccatgat cgcgaccagg
     4441 gcgaaggtca gcggccagag gctgcgcggc cgctctcgcg gcggcccgcc cccctgcggt
     4501 acgggtggcg gcgccaccac agggacgggg atggaggctg ccggcgcatc gaccacgggc
     4561 tgcgccgcgg tgagccgtgc cgtgggctgg gtgctctcct ccagccgttc gccgtgctgg
     4621 atgatggcca cggtgatgtt atcctcgccg ccgcgctcgc gggccagccg caccaggatg
     4681 tcggcggcca cgtccggccg ctcgtcgccg gcaatggcgg cgatctcgcc gtcctccacg
     4741 tgccgggtca ggccgtcgga acagagtacc agcaggtcgc ccggctccag gacgatgggg
     4801 tagagatcga tgggcggccg cttctccgag ccgatgctat agagaatgac gttcttgtag
     4861 gggtagtgga cggcctcttc cggcgtcaga gcgccctctt ccaccaggcg ggcgacgagg
     4921 ctctggtcgc ggctgatctg ttccagccgc cccttgcgcc actggtaggc gcggctgtcg
     4981 ccaacgttgc cgatgaatgc ctggccatcc tggatgacca cggcgaccat cgtcgtggcc
     5041 atgcgccggc tgtcgtcgcg gccagcggcc aggcggcgca ggtcggtgtt ggccgcctgc
     5101 atggcgtcga tgagtcgctg cccccaatcg gtgcggccat cggcggccag gtagtggtgg
     5161 agcgtccgct cgctggcgta ctggctggcc acttccccgg cgtcggctcc ccccacgcca
     5221 tcggcgacga tgtagaccgc gccgtggcgg cggcgctcgt cctccgtggc tggctcgtag
     5281 accttaacaa agtcctcgtt gtggccacgc accagaccgg tgtcggtgcg ccaggcggcg
     5341 gaaagggaga tcgtctctgc catcacgggc actatagcaa aaccccaccc ggcggcaaga
     5401 gcaacaaccc aacgattgcc tgacgctcgc ccacacctga caggtctggc ctctcggtta
     5461 caatagggta acgttgccag cggcccatac acccaccgcc ggccaagacc aacagcagga
     5521 gatagcctat gcccgccgac aaccgccgcc cccactccga aacgcgcacc atccgtaccc
     5581 gcgcccgcgc cagccactac cgcgagcagg tgacgcccat ctatatggcc tccggcttca
     5641 ccttcgacag cgccgaccag gcccgcgccg tctacggcgg cgaggaaccg ggctatgtct
     5701 atacgcgttg ggacaacccc aacagcgctg aactggtagc gcggctgtgc ggcatggaag
     5761 gggctgaggc ggggctgccg atggcctcgg gtatggccgc gctcttcacc atcctggccg
     5821 ggctgctcaa tagcggcgat cacgtggtgg cctcccgctc gctctttgga gcgacccacc
     5881 agctgctgac gaccatcctg ccacgctggg gcatcgccca cagctacgcc gactgcgagt
     5941 tgaccgagac gtggcccggc ctcttccgcc cggagacgcg cctgtgtctg gtggagacgc
     6001 cctccaaccc cgacctgacg ctggtcgatt tggcctgggt ggcggcgctg tgtcgcgagc
     6061 gcaaggtctt gctggtggtc gataatacct ttgccacgcc ggtggttcag cagccgctgg
     6121 cgctgggcgc ggaccttgtc gttcactcga cgaccaagtt cctcgacggc caggggcgca
     6181 ccatcggggg cgtcatcctc ggcgaccgtc tgacggttaa cgagctgacc catttcgccc
     6241 gccagacggg accaacgttg tcgcccttca atagctggct gctcagccag tcgctggaaa
     6301 cgctgtcgct acgcatggag cgccattgcg ccaatgccct ggccctggcc gagtggctgt
     6361 cgcggcgggc cggcgtggag acagtgcgct atcccttcct gccgtcgcac ccgcaggccg
     6421 agctggcgcg gcggcagatg tcggccgggg gcggcatcgt caccttcgag gtcagcggcg
     6481 ggctgccggc cgggcggcgc tttctggacg ggctgaagat gtgcgacatc gtcgccaatc
     6541 tgggcgacgc gcgcacgacc gtcacccacc cggccagcac cacccacagc gctctgaccg
     6601 acggcgagcg ccgcgtcgtg ggcatcaccc ccggcctgat tcgcgtctcc gtcgggctgg
     6661 aacatatcga cgacatcatc gccgacgttg accaggcgtt gggaaattag gaattaggag
     6721 tgaaggcccg gccagtcagg ggcagtagcc agttcgttgg ggcgcgccgg ttccaggcca
     6781 gcgcttcggc cggcggggcg acgatggcca acatgtgggc cgcgtcgatg gcgtagaaag
     6841 cctcggtcgg gtcagactcg gccagggcgg gcggagcgtc gggcggcagc gcgcaagcgc
     6901 gtctcagtgg ccgccggagc attgaaatcc agccgcgcgt ccagatcggc ggaggaatgg
     6961 atcgtcataa cccggaatga tagccagcaa aaacgccgcg cccaaacgag ttgggcgcgg
     7021 cgccttgcta caggaggaga aagaaaatga gaagcgtttt tgctgtccgt tctgccatct
     7081 agatgactgg cggtctcatg ttcttacccc ataggtcaac tggttacggc tgcaactccc
     7141 agacgcgggc ctcgccgggc gagccggggc cgtagagcca cagcacaccc gtctccgtca
     7201 tattcagctg gtcgccgaag tcgataatcc cgaagcgcgt ccgcggcgaa cgtgccggca
     7261 gccaggtgaa accgatgtcg ccgttctgga acacagtcag gtagcgctcg cccaacggca
     7321 cgatgttcat accttcgatg gtgtcgatcg agccactgta gtagtagtcg aaagcaaacg
     7381 cggatgcgtc catcgaggtg ttgaagaagt tcgacgcgtt catgccgatc tgctggccac
     7441 agaagaagag cacgaagttg ccggagttgg tctcgtgctg tgtgtagaag gaggcgctgg
     7501 cagcgccggt ggccaggttg ctgacccagg tcaggctgcg gccgtcgctg ggggcgctac
     7561 caaacgcgcc ggaggcgtcg tagttgaaca cctcgtaatc gaacgtgccg tcgccgtcga
     7621 ggtcaaggtc gaagccgacc aggacgtggc tggcgtggga gtagcgatcc caggtattga
     7681 ccgccagacc catgacgaag gagtccacgg ggccgcagac gccggccgga accgggtagg
     7741 tctgcacgcc gacccaacgc aggtcaaccg gggcagtctg gccgcccagg ggcgggtcgc
     7801 cagcgatctg cgggctgctg cccagcagcg agtaggtgtc gatgtacatc ggcgatacgc
     7861 cggtgttgcg cacccaaccg gtgttcgtct ggggcacgaa cccggtctcc ggacgcccgg
     7921 cagcccgcgg caggacgtgc caggccacat gggcgtccgc gccctgtccg ttggccccga
     7981 cgccgatgaa gcggatatag ccgtcatact cgaccagcgt cagcgcatcc gggttagcgc
     8041 cctggctgcc gctgttgagc acccactcgt gcaggggccg gctcaaccgc ggccggataa
     8101 acatgcgcac gggaacgccc acagtgccat tggccggaac cgtgatcagc gtgggcaccg
     8161 tcacgcgcac ttcgccgttg gcgtcattgt cgtagcggta atccacttcc gagcgcagcg
     8221 ttacggggct gtcggtgtag ttgcggatgg tgacgcgctt gagcatgtcg agctggcctg
     8281 tcacctcatg gaagccaaac gacagcgagc cggtcagatc atcgcgatcc caggccgcaa
     8341 tgggcgagtg gagcgcgcga tcgacgcgca cttcgccgcc gccgatgcgg ctgatggctg
     8401 ccaggtagcc gccgaagaac tccggcgtat tcatgatgtc cgtctcagcg gtattcatca
     8461 agagcgactt gatctccgcc caggagcgat ccgggtaggc gtccatcaac agagccgccg
     8521 agccagtcac catcggggcc gcgccggacg tgccactgaa ggcctccgtg cccgtgcccg
     8581 tgccgtggat ggccgagacg gacgcgccgg gtgcgccgat gtcgggcttg gccatgttga
     8641 ggccgccgct cgggccgcgg ctgctgctgc cgaccatgtg gccgaccagc gggatgtcat
     8701 tggccgggtc gaaagcgacg atcacctggc cctcttccag agcatccttc agcagttggg
     8761 catcagcctg gctgatcatg aagccgggga cgctcggatc accaccgcca tagctgcccg
     8821 cgaacggttc gccgggggtg atcaggccaa tgatgccggc catcgcgccg cccgcggcga
     8881 tgttgctgat cttgatactg aacgcgcacg tgccgcgatc caccaggacg atcttgccgg
     8941 tcagcgaacc agccgggaag gcagtgcagc catcgaggtt gccgccactg ccgtcgccat
     9001 actgcaacgg ggcctggatc gggtctgtga cttcaggcga ccagggctgg tgcacggccg
     9061 cgtacaaacc ggcgatgctc tccggctcga caacttccat caagccctgc gtcgccgaag
     9121 ggacgttggt ctgggccaca gccagtgctg tgaccgccgc ggcgggcgtg ccgtgggcat
     9181 agggcttgtc ggcgctattg ccggccgagg ccaccgtcag gacaccggcg gcactggcgt
     9241 tgtccaccgc gtaggacagg tcatcccagc gcgggtcgcc atagggcgag ccgagggaca
     9301 tgttgatcac gtccaaatgg tcgctcgtgt cgccatcgtt attggggtca acggcaaatt
     9361 caatggcctg gagcaagggc agcccggcgc aggaggtcga gaccgaggag caggccttca
     9421 gggcgtagag atcgacgccg ggggccacgc cgaggttgcc gccgatgatg tcggccacgt
     9481 gcgtgccatg gccgccgccg gggccgtcat ccaacgggtc ggggtcgggc atgatgtcgc
     9541 cgttgggcca ctcgctgccg acgaagtcat agccgccgac gacgcgctcg gtcgggaagg
     9601 ttcccggctc gatgatcgtc gggtcgttgt tctcgtaggc ttcgactgtg ccggggccgc
     9661 cgaaaacggc gtgggtgtag tcgatgccgc tgtcgatcac gccgacacgg acgccagagc
     9721 catcgtagcc ctcgttctgg accgcggtcg cgccgatgta gggcacggtc tccgacagat
     9781 ccagttcgta gttacggagc ggggtgatgg cgacgacgtt ggggttgcgg gccaactggg
     9841 ccagggtggc gctgtcggcc tcgatgacga cggcgttgag cgccatgcgc agcgtggcca
     9901 gcacgcggac gttgctatcg agcgagcggg ccgcggcgac gacttcggcc tgctgcgaga
     9961 cggccgcggc catcgcgcct tcggcggccg gagccaccgc cagcgccggt tggctcagac
    10021 ggacgacgac ctgctgccgg ccggtggcgt tgcgcagttc cggcgctagc cggaaggccg
    10081 cttcggcgcc gcttacctgt atgggcgtgt ccagtctaag ggttttgacc tgatcaggaa
    10141 tggtgcgccc ggtttcagcg cgtgagggct gcgtagcgtc ctgggcaaag gcaattgacg
    10201 cggtaatcag cagcagaccg acaatcacca ctagcttaag agtgttcttc ctagacaact
    10261 tggtcctcca aggattcgtt gagcaaaagc atcggatcat tgaccggggc ggccggaacg
    10321 gctggtcgca gaccggtgga acggtgtgga gggcttgcgg atcagttggt ttttgcgggt
    10381 agcacgacct ccttgccggc agccggcagc tgaggagcag tcactgatga tgtcgctatt
    10441 gtcagaatgg gcatgcaaca gccacacaga aaggacatta ccccattcaa aacacaggta
    10501 acgtcagtag tttagtagcc gatgcttaca aactctctta caattctttc acggattgtt
    10561 cacgcttagc ttatcctggt gggcgcatcg gtcaagggca gaccagcggc taaggtggac
    10621 aggcagacaa acatacgtac aatcgtttat tgacagtgga taccgttata caggcttctg
    10681 ggccacaagc ggcaatcggt aaggaggttg gaggatatgg aacgtctgcg acgcgatttc
    10741 agcacgatca cgctggtcat gatcccggtg gccattgcca tcaatatcgc catcggccag
    10801 ctcatctaca cgctcaaagt gcctctctat ctcgactcca ttggcacgat cttcgttgcc
    10861 gccgtggccg ggccgctggc cgggatgctg accggcgcat tgaccaacct ggtctggggc
    10921 ctcagcggca tcaactcgtt ctacaccgcc ttctggatca ccggcgctgt tatcggcctg
    10981 gtggcggggc tgttctcgcg ctttggctgg ttcaaggtgt ggtggcgagc catcatcgcc
    11041 ggcgtcatca ccggccttat cgccgccctc atctccgcgc ccattctcac ctacgtcaac
    11101 ggcggcgtca ccggctcggg caccgacgtg atcaccgccg tctttcgcaa tgctggcctg
    11161 ggcatcctgc cctcagcctt cgcccagggc gtctcgtccg acccgctgga caaggccatt
    11221 tcgttcttca tcgtctgggg cgtcattcgc gccctgccgt tgcgcttcct ggatcgcttc
    11281 tcgcgggctg aagacatccg ctaaggcggc tgtctggcga ctatgagcac atccttcagc
    11341 ctgtacattc cgcgcgagag cgggatgcac cgcctacacc cgctgaccaa actcgctctc
    11401 gtcgctttct gtctggtgct ggggctgttt ctgccggggg tctggttgac ctacctggcc
    11461 tttatgctgc tcatcgtgcc gctggccgcg tgggcggggg tgttgccgtc gttcctgcgc
    11521 gccgttttca agacggccct gcccgtggtg atttccgtgt tcctggtgca ggggctattc
    11581 tggccgggcg gcacacccat cttgcacctg gggccgctgt cgctgaagca ggagggcttg
    11641 cagtttgcca tccgcagcg
//
LOCUS       DAOSMQ010000026        11525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5585_length_11525_cov_4.860157, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000026 DAOSMQ010000000
VERSION     DAOSMQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5585_length_11525_cov_4.860157"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PK829_01675"
     CDS             <1..703
                     /locus_tag="PK829_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU39953.1"
                     /translation="ASLESTVSRITLRPPQDIGKIAAIYMPYRSTDKTRQKKDAKRTA
                     MMQAAVRVFAEKGYHAATVRDIVSEANVAIGTFYFYFPDKETLFVHLFEETAAFLLQA
                     IDQALMSRSSYPAQLETAIQSYVNVAFYEPAVVQLLLVGGMGSVPSLSERRAEYREKL
                     VRAWQRALTAALERHQIEPQNTRRTAEALVGAFDEIVYNLLSSPLAEQERSVAVRDMV
                     QFGLRAAGYLYTPGD"
     gene            complement(718..3744)
                     /locus_tag="PK829_01680"
     CDS             complement(718..3744)
                     /locus_tag="PK829_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOU39954.1"
                     /translation="MIPHYLSLTNFLSYRDTAVLDLSSVHLACISGLNGAGKSSLLDG
                     MTWALFGRGRSGDDNVVNRIAAGNGKAAEVTFIFELEGAVYRVIRRKVVGKTTQLELH
                     VRGALADSDALADSDKGQPRWRTLTQAGVRETQRAIEELLRMQYDIFTNASFLLQGQA
                     DEFTTKTPNRRKEILADILGVNRWDAYKTLAGERRKAAENEEKVIDRRLADIDAELDQ
                     EGERRRELELAEAYEQAVAAQQTAQDALVATLREKRALIEQQRQQVTQTTTELERTRT
                     ELRRLERDAALRQADLEGHRALIDRAAAIAAAYQAYQQLAAELSMWQKRADDANALLR
                     ERQPFELAIAKARTALEERVQTLMAQETAAADALAGRQRIAAQLAERRTQLDTAEAEV
                     VELTEREVAWHAARDRRQQAEANSALWQLERDQLAAQAEEAERSRAQREMIAVSHDQA
                     QARLDAAQAILATLAGREERRAAIKFERADRNGELEALRREGEERNERIERLRLEEAT
                     GECPICGQPLTEEHREAVLKDLEDEREASRSRYREVQAQLQALDQEETVLSAEATRRP
                     HHEKERDTQQRSVANYAAQLAHIDGLLSVWDSRDGRSRLAALEALLAAADVAALRAEE
                     ERLRSATADLRQKNREQQLLVGEIRRLETQCEQLDKTAQQWAQTGRPALLAARRELEE
                     QRYAADAQARLAAVDKRLAAVAYDEAAHAAARGRLAEQSAAPEEYQALQRAEAAIKPL
                     ADGLAELERRREREAARAADLETQHISLSASLRDLQAGATDLRAAEAELARLRDEQAR
                     AIRATSAARQKVDVLADLRRVQKDLRAERQTVGGRIGFLRQLEDACGRNGVQALLIET
                     ALPEIEEHANELLYRLSGGEMRVRFDTQRERKSDGGQIETLDIKISDSSGERPYENYS
                     GGEKFRVNFAIRLALSQVLARRAGARLQTLVIDEGFGSQDPEGRQRLVEAINAVQPEF
                     ACILVITHIDELRDKFPARIEVEKTAAGSRLALVAV"
     gene            complement(3775..5013)
                     /locus_tag="PK829_01685"
     CDS             complement(3775..5013)
                     /locus_tag="PK829_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOU39955.1"
                     /translation="MSVRILHFADAHIDIANYGRHDPQTGLPQRVVDFLAALDQIVDA
                     ALSERVDLVIFAGDAYKDRNPQPTFQRAWGERIMRLSRAGIATLLLIGNHDIAPAENR
                     AHTLQEFATLSVPHVFVADKLKLWTPAELGVPVQVLALPWVTRSRFLARYDMTGKTLD
                     EIHDAILDRIHEALTSTLENEVDPALPLILTAHASVHGARLSSERNIMLGQDLTLSEG
                     LVSDPRFDYVALGHIHKHQVLNERPPVIYSGSIERIDFGEAGEQKGYVLAEVSRGHTD
                     WRFVPLHTRRFVDRLISIKDPDSFMDDIMSQLPEAEHIAGAICRVQIEFAQEHEALLD
                     EKLIRDHFADAFDLRLLKHRLGSEKSRLPSGLAVETLSPYELLEFYWRYKGRPDAEID
                     ALLELAHDVLAAGADGDAVV"
     gene            complement(5076..7091)
                     /locus_tag="PK829_01690"
     CDS             complement(5076..7091)
                     /locus_tag="PK829_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39956.1"
                     /translation="MRRSLVSIFALLGLIVLPMFIARAWADDLPSLQTDEPLIVAEPP
                     SVWLPDEVHIDDLPQWTAAAPAADTCATATPLELSFSHTADGSGTLTNSFTADATDPV
                     LSCAYGAPTSPTGYRTAWHVLVAGDTGVITITTEGSDYDTILAVYEGTCEALRSLSCA
                     DDTQGFQSRTTVRVIRGHTYYILVADYQSGAPASAILRLSAIMREGGQRWRQVSNLPF
                     GGVSRHAFASDGVDMYVIGGQTRIQGVPVLTNRLLRYNAESRQWTKLADIPGTGLSNT
                     TAVRLGRRIYVPGGFNGNTSDYANTHFVYDLDTDFWGTAAPIPTALLPGGEMFAWASA
                     VAAPDETSYYLTGGLASIPPFQTNAVVLNNTYRYTPSTNQWEAFRPMTTARYAHTAAW
                     VSTANRGLCVVGGLSSGVDTAGQPIIVLLTSGECYNPATGSDWRPTGPLNFPRYNAGS
                     AIGPDGNWYVFGGLDAQGNGVPEVEIYDPLANAWHVLDSDFSWGGRPTDPPFDWPRGA
                     FWRDNLYVFGGNTPREQRVVSTVDRLNVGSGVIWYVHRALIPIAPKIGSDNLLFWATY
                     LPLNSPLNGNFVRSNQFYNAYYFAWPAFGRATIRLGNIPSDSNFNVAVYDANKVLRGQ
                     GDPAIYGGEKTVQITLMPGTYYVVVQRIFPKDLPNPDDFYSLGVFTP"
     gene            7222..7725
                     /locus_tag="PK829_01695"
     CDS             7222..7725
                     /locus_tag="PK829_01695"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HOU39957.1"
                     /translation="MSVTAITDTLVVAAWPTAEAITALRTLNVHLVISMTNRVPPPEL
                     SEPPFRLLHLPTNDSPLRPVPMGKLCTGVTAALPVIARGQGVAVHCSRGRHRSVAMAC
                     AILIGLGYSAEAAMFLVKARRAEADPSIWYIRRRITKFGKIWPQGCSAQKTRDDALRE
                     HLSTGGV"
     gene            7758..8720
                     /locus_tag="PK829_01700"
     CDS             7758..8720
                     /locus_tag="PK829_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU39958.1"
                     /translation="MNESTRTIQIIINPAAGNNEPVLNTINDVFSQNDGIQWDVRVTH
                     GPGDATEMAHQAAAAGCDLIAVYGGDGTLMEVINGLMAHGHQTPIAILPGGTGNTVAA
                     DLGIPHNLADALHVVATSQGRRQLDIGEVNGRYFLLRAYTGIGSDHNASRELKDHLGI
                     LAYPIKGLQFLKEQPPAAFRITVDTEKIEEPGVLCYVNNIAYSRAPQLQDWLERVFLD
                     VQVHDGDESSSAEEPMLQTIDPTDGLLDVVLLTQNPFDLHAIKSCVVRSGEEQATVHF
                     MQGKRIHIDADPPQTLWIDGEECGTTPAYLQVVPQAITVVVPEG"
     gene            complement(8766..>11525)
                     /locus_tag="PK829_01705"
     CDS             complement(8766..>11525)
                     /locus_tag="PK829_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU39959.1"
                     /translation="CGTAISQHEIVTDGYFDVTHDSVFVRFPLVDRPNEALLVWTTTP
                     WTLTSNVAAAVGPELDYVQVRAEDGWTYYLAEGAMKNSLVGKNNEVVGRLKGADMLGW
                     QYTGPFDDLPQVVAAFDEKDYSHRVIPWTEVGAEEGTGIVHIAPGCGAEDFALGQEFD
                     LPVVAPLDENGVYLPGFDWLTGREVLGGPNTPSVAEDIFTALREGGFFYRKQKYTHRY
                     PHCWRCGSELVYRLVDEWFISMGPVYDKPREQLTAEEKTASLRYQIMDRVERINWVPG
                     FGYDREMDWLRNMHDWMISKKRYYGLALPIWECEECGTFTVVGDEHELEERAVEGWDT
                     FAGHTPHRPYIDAVKIACPSCGATTSRIADVGNPWLDAGIVAFSTLHYRTDRAYWEKW
                     YPADWISESFPGQFRNWFYSLLAQSTVLTDEPPFDNLFGYATLVDEDGRPMHKSWGNS
                     IEFNEAAEEMGADTMRWLYASCKPEQNLQFGFNRGDETRRRFLIPLWNVYSFLVTYAN
                     ADGWTPQAAAEQGSTGEASSPGPRPLAPGTSQLDRWIVERLNETTVDVREALDAWDAE
                     RAAAALELLLDDLSNWYVRRSRRRFWKSEADADKAAAYATLYHVLVEFIKLLAPFLPL
                     TTEAMYQNLVRAGDAGAPLSVPVLSVPLLSVHHHFYPQADAAALDHRLLDKMRLVIDA
                     AALGRSARGSADVKLRQPLARARVFVGSEQERADLQELAGVLAEEINVKAIDIVSEVG
                     ELVSYKLLPNNRALGPKLGPLFPAVRAALAQLDPAAAAVRLQAGAGLELAVNGQMVTL
                     NGDDVLVQTESRGGLAVAGGKGITVAIDPHLTPELVQEGYARDIVRAINSLRKDAGLE
                     VSDRIALHYVAEGDVAATFQNFGGFIGAETLATQLMAGEPDNDDIRQQVSIGDTPVTL
                     ALHKL"
BASE COUNT         1906 a         3729 c         3840 g         2050 t
ORIGIN      
        1 ggcgtcgttg gaatccacgg tcagccggat cacattgcgg cctcctcaag atatcggtaa
       61 gatagcggcg atctatatgc cctaccgcag caccgacaag acccgccaga aaaaggatgc
      121 caaacgcacg gccatgatgc aagccgccgt gcgtgtcttt gccgagaaag gataccacgc
      181 ggccactgtc cgcgacattg tgagcgaggc caacgtcgcc atcggcacgt tctacttcta
      241 cttccccgac aaggagacac tcttcgtcca cctctttgag gagacggccg cttttctgct
      301 acaagccatc gaccaggcgc tcatgagccg gtcgtcctac ccggcccaat tggagaccgc
      361 catccagtcg tatgtcaacg ttgcctttta tgagccggcg gtggtgcaac tgctgctggt
      421 cggcggaatg gggtcggtgc cgtcgttgag cgagcggcgg gcggagtatc gcgagaagct
      481 ggtgcgtgcc tggcagcggg cgctgacagc cgccctggag cgccaccaga ttgagccgca
      541 gaacacacgc cgcacggctg aggccctcgt aggcgcgttc gacgagatcg tctacaactt
      601 gctctcctcg ccgttggccg agcaggagcg gtcggtggcc gtgcgcgaca tggtccagtt
      661 cggcctgcgc gccgccggct acctctatac cccaggcgac tgattcgccc ccaccttcta
      721 aacggcgacc agcgccaggc gcgagccggc ggccgtcttc tccacctcga tgcgcgcggg
      781 gaacttgtcg cgcagttcat cgatgtgggt aatgaccagg atgcaggcaa actccggctg
      841 cacggcgttg atggcctcca ccaaacgctg ccggccctcc gggtcctggc tgccgaagcc
      901 ttcgtcgatg accagcgtct gcaagcgcgc cccggcccgg cgggccaaga cctgcgacag
      961 ggccaggcga atggcgaagt tgacgcggaa tttctcgccg ccgctgtaat tctcgtaagg
     1021 gcgctcgccg ctgctgtcgg aaatcttaat gtccagggtc tcgatctggc cgccgtcgga
     1081 cttacgctcg cgctgcgtgt cgaagcggac gcgcatctcg ccgccggaga ggcggtaaag
     1141 cagttcgttg gcgtgctcct cgatctccgg cagggctgtc tcgatcagca acgcctggac
     1201 accgttccgg ccgcaggcgt cttccagttg gcgcaagaac ccgatccgcc cgccgacggt
     1261 ctgccgttcg gcgcgcaggt ctttctgcac ccggcgcagg tcggccagga catcaacctt
     1321 ctggcgggcg gcgctggtgg cgcggatggc gcgggcctgc tcgtcgcgca gacgggccag
     1381 ctcggcctca gcggcgcgca agtcagtagc gccggcctgc aagtcgcgca acgacgctga
     1441 taggcttatg tgctgcgttt ccaaatcggc cgcgcgcgcc gcctcgcgct cgcgccggcg
     1501 ctctagctcg gccagcccgt cggccagggg cttgatggcc gcctcggccc gttgcagggc
     1561 ctggtactcc tccggcgcgg cgctctgctc ggccagccgt ccacgggcag cggcgtgggc
     1621 ggcttcgtcg taggccacgg ctgccagccg cttgtcgacg gcggccaacc tggcctgcgc
     1681 gtcggcggcg tagcgctgct cctccagctc gcggcgcgca gccagcagcg ccgggcggcc
     1741 tgtttgcgcc cattgctggg cggtcttgtc cagctgctcg cactgcgtct ccaaccgacg
     1801 aatttcaccg acgagcagct gctgctcgcg attcttctgg cgcagatcgg ctgtggccga
     1861 acgcaagcgt tcttcctcgg cccgcaacgc agccacgtcg gccgcggcaa gcagagcctc
     1921 cagcgccgcc agccggctgc gcccatcgcg gctatcccag acgcttagca gcccatcgat
     1981 gtgggccagt tgcgcggcgt agttggccac gctgcgctgc tgggtgtcgc gctccttctc
     2041 gtggtgcggc cgccgggttg cctcggcgct caggactgtc tcctcctggt caagcgcttg
     2101 cagctgcgct tgcacttccc gatagcgtga ccgcgacgcc tcgcgctcgt cttccaggtc
     2161 cttgagcacg gcctcgcggt gctcctcggt cagcggctgg ccgcagatgg ggcactcgcc
     2221 ggtggcctct tccaggcgca ggcgctcaat gcgttcgttg cgctcctcac cctcacgccg
     2281 tagcgcctct agctccccgt tgcggtcggc gcgctcgaac ttgatcgccg cgcggcgctc
     2341 ttcgcgcccc gccagcgtgg cgaggatggc ctgggccgcg tctagccttg cctgggcctg
     2401 gtcatggcta acggcgatca tctcgcgctg ggcgcggctg cgctcggctt cctcggcctg
     2461 cgcggccaac tggtcgcgct ccagctgcca caaagcgctg ttggcctcgg cctgttgccg
     2521 gcggtcacgg gcggcgtgcc aggccacttc ccgctcggtc agttcgacga cctccgcctc
     2581 agcggtgtcc agttgggtgc ggcgctcggc gagttgcgcg gcgatgcgct gccggccggc
     2641 cagagcatcg gccgccgctg tctcctgggc catgagcgtc tgtacgcgct cctccagcgc
     2701 cgtgcgggcc ttcgcgatgg ccagttcaaa tggctggcgt tcgcgcagca gggcgttggc
     2761 atcgtcggcg cgcttctgcc acatgcttag ctcagccgca agctgctgat acgcctgata
     2821 ggccgccgca atggccgcgg cgcggtcgat cagcgcgcga tggccctcca ggtcggcctg
     2881 gcgcagcgcg gcgtcgcgct ccagccggcg caattctgtt cgcgtccgct ctagctccgt
     2941 tgtcgtctgc gttacctgct ggcgctgctg ttcgatgagc gcgcgctttt cgcgcagcgt
     3001 ggccaccagc gcgtcctggg ctgtctgttg ggcggcgacg gcttgctcat acgcctcggc
     3061 caactccaat tcgcgccggc gctcaccctc ctggtccagt tcggcgtcga tgtcggccag
     3121 gcggcggtcg atgaccttct cctcgttctc agccgctttg cgccgctcgc cggccagcgt
     3181 tttgtaggca tcccagcgat tgacgcccag gatgtcggcc aggatttcct tgcgccggtt
     3241 gggcgtcttg gtggtgaact cgtcggcctg cccttgcaac aggaagctgg cgttggtgaa
     3301 gatgtcgtac tgcatccgca gcagttcttc aatagcccgc tgcgtttcgc ggacgcccgc
     3361 ttgtgtcagc gtgcgccagc gcggttggcc cttgtcgctg tccgccagcg cgtcgctgtc
     3421 cgccagcgcg ccgcggacgt gcagctccag ctgcgtcgtc ttgcccacga ccttacggcg
     3481 gatgacgcgg tagacagcgc cctctagctc gaagatgaag gtcacctcgg cagccttgcc
     3541 gttgccggcg gcgatgcggt tgaccacgtt gtcgtcgccg gagcggccgc ggccgaagag
     3601 cgcccaggtc atgccgtcca gcaggctcga cttgcccgcg ccgtttagcc cggagatgca
     3661 ggccagatgg acgctgctca ggtcgaggac ggccgtatcg cggtaggaca ggaagttggt
     3721 caggctcaag tagtggggga tcatggccgt gtgcgagtgt cgtcgcggca gcgctcagac
     3781 aacggcgtcg ccgtctgcgc cggcggccag cacgtcgtgc gccaattcga gtagcgcatc
     3841 gatctccgcg tctggccggc ctttgtagcg ccagtagaac tccagcaact cataggggct
     3901 gagcgtctcc acagccaggc ccgacggcag acggctcttc tcggagccaa gacggtgctt
     3961 cagcaggcgc aggtcgaagg cgtcggcgaa gtggtcgcga atgagcttct cgtccagcag
     4021 cgcctcgtgc tcctgggcaa attcgatctg cacgcggcag atggccccgg cgatatgctc
     4081 ggcctccggt agctgggaca tgatgtcgtc catgaagctg tcggggtctt tgatggagat
     4141 aagccggtcg acgaagcggc gcgtatgcaa ggggacgaag cgccagtctg tgtgcccgcg
     4201 cgacacctcg gccaaaacgt agcccttctg ctcgcccgcc tcgccgaagt cgatgcgctc
     4261 aatcgagccg gagtagatga ccggcggccg ctcgttgagc acctggtgct tgtggatatg
     4321 gcccagggcg acgtagtcga agcgcgggtc gctgaccagg ccctcgctga gcgtcaggtc
     4381 ctggccgagc atgatgttac gctcgctgct caggcgcgcc ccgtggacgc tggcgtgggc
     4441 ggtcaggatg agcggcagcg ccggatcgac ctcgttctcc agtgtcgagg tcagcgcctc
     4501 gtggatgcgg tcgaggatgg cgtcgtggat ttcgtccaac gtcttgccgg tcatgtcgta
     4561 gcgggccagg aagcggctgc gcgtcaccca gggcagggcc agcacctgca cgggcacgcc
     4621 cagttcggcc ggcgtccaca gcttcagctt gtcggcgaca aagacatggg gcacagagag
     4681 agtggcgaat tcctgcaacg tatgggcgcg gttctcggcc ggggcaatgt cgtggttgcc
     4741 gatcagcagc agcgtggcga tgcccgcccg tgacaggcgc atgatgcgct cgccccaggc
     4801 gcgctggaag gtcggctgcg ggttgcggtc cttgtaagca tcgccggcga agatgaccag
     4861 gtcaacgcgc tcggacaggg ccgcgtcgac gatctggtcg agcgcggcca ggaaatcgac
     4921 gacgcgctgc ggtaagcccg tctgcgggtc gtgacggccg tagttggcaa tgtcgatgtg
     4981 ggcgtcggcg aagtgaagaa tgcgcacgct cataggtcaa tgtgcgcccg cccggccgcg
     5041 cggccgggtg ggcaagacgc tgccgccgcg ccgggtcacg gcgtgaagac ccccaggctg
     5101 tagaagtcat ccgggttagg taagtctttc ggaaagatgc gctggacgac gacgtagtag
     5161 gtgcccggca tcagcgttat ctggacggtc ttctcgcccc catagatggc agggtcgccc
     5221 tgaccacgca ggactttgtt ggcatcgtag acagcgacgt tgaagttgct gtccgaggga
     5281 atgttgccca gacggatggt cgcccggccg aaggcaggcc aggcgaaata gtaagcgttg
     5341 tagaactggt tggagcgaac gaagttgccg ttcagcgggc tgttcagcgg caagtaggtc
     5401 gcccagaaga gcaaattgtc gctgccgatc ttgggcgcaa tggggatgag ggcgcggtgg
     5461 acataccaga tcacgcctga gccgacgttg agccgatcga cagtcgagac gacgcgttgt
     5521 tcgcgcggcg tattgccgcc gaagacgtag agattgtccc gccagaacgc gccccgcggc
     5581 cagtcgaaag gcgggtccgt cggccggccc ccccagctga aatcgctgtc caggacgtgc
     5641 caggcattgg ccagcgggtc atagatctcg acttcgggca cgccgttgcc ctgcgcgtcc
     5701 aggccgccga agacgtacca gttgccgtcc gggccgatgg cgctgccggc gttatagcgc
     5761 gggaagttga gcggcccggt cggtcgccag tctgagccgg tcgccgggtt atagcactcg
     5821 ccgctggtca gcagcacgat gatgggctgg ccggcggtgt caacgcccga tgacaggccg
     5881 ccaacgacac acaaaccgcg attggccgtg ctgacccagg cggccgtgtg ggcgtagcga
     5941 gccgtggtca tgggccggaa ggcttcccac tgattggtgc tgggcgtgta gcgataggtg
     6001 ttgttgagga caacggcgtt ggtctggaat ggcgggatgc tggccagccc accagtcagg
     6061 tagtaggacg tctcgtctgg cgcggcgacg gccgacgccc aggcgaacat ttcgccgccg
     6121 ggcagcagcg ccgtcgggat gggcgcggcc gtgccccaga agtcggtatc caggtcatag
     6181 acgaagtggg tgttggcgta gtcgctggtg ttgccattga agccgccggg cacgtagatg
     6241 cgccgaccca gccggacagc ggtggtgttc gataagccgg tgccgggtat gtcggccaac
     6301 tttgtccact ggcgcgactc agcgttgtag cgcagcagcc ggttggtcag tacgggcacg
     6361 ccctggatgc gcgtctggcc gccgatgacg tacatatcga cgccgtcgct ggcgaaggcg
     6421 tggcggctga cgccgccgaa gggcaggttg ctgacctgac gccagcgctg cccgccctcg
     6481 cgcatgatcg cggacagccg caggatggcc gacgcgggcg caccagattg gtaatcggcg
     6541 acaagaatgt aataggtgtg cccgcgaatg acgcgcaccg tcgttcggga ctggaagccc
     6601 tgcgtgtcat cggcgcagga aagcgagcgt agagcctcgc atgtaccttc atagacggcc
     6661 aggatggtgt cgtagtcgct accttccgtg gtgatagtga tgacgccggt gtcgccggcc
     6721 acaagtacgt gccaggcggt acgatagccg gtcgggctgg tcggcgcgcc ataggcgcac
     6781 gagagaaccg ggtctgtggc atcggcagtg aaggagttgg tcaacgtgcc gctgccatcg
     6841 gcggtatggc tgaaagacag ttccagcggg gtggccgtgg cacaggtatc cgccgccgga
     6901 gccgcggccg tccactgggg caggtcatcg atgtggacct catcgggcag ccagaccgac
     6961 ggcggttcgg cgacaatgag cggctcatcc gtctgaagtg agggtagatc atcggcccag
     7021 gcccgcgcga tgaacattgg caggacaatc agccccagaa gagcaaagat ggaaacaagc
     7081 gagcgacgca tataatcgcc aatgataaca acccttgtcc gaaaaggcac accggcatag
     7141 ccggcctata tcatcactgg cgaacgaatg tatctctgac attttgacga accagccggc
     7201 tttttcctat gatgatgacc gatgagcgtg accgcaatta ccgacaccct ggtggtcgcc
     7261 gcctggccga cggccgaagc catcaccgcc ctacggacac taaatgtcca tctggtcatc
     7321 agcatgacca accgcgtgcc gccaccggaa ctgagcgagc cgcccttccg gctgctgcac
     7381 ctgcccacca acgactcgcc cctgcggccc gtgccgatgg gcaagctgtg cacgggggtg
     7441 acagccgccc tgccagtgat cgcgcgcggt cagggtgtgg ccgtccattg cagccgcgga
     7501 cgccaccgca gcgtagccat ggcctgcgcc attctcatcg gcttgggcta ctcggccgag
     7561 gcggccatgt ttcttgtcaa ggcgcgccgc gcagaggccg atccctccat ctggtatatc
     7621 cgccggcgca tcaccaagtt cgggaagatt tggccgcagg gctgtagcgc tcagaagacc
     7681 cgcgacgacg cgttgcggga acatttatca actggaggtg tctgaagaac aactgcttgg
     7741 cttcgtaaag gtgacgtatg aatgagtcaa cccgtaccat tcagatcatt atcaatccgg
     7801 ccgccggcaa caatgagccg gtgctcaaca ctatcaatga tgtcttctcg cagaacgacg
     7861 gcatccagtg ggatgtcaga gtgacccacg ggccgggcga tgccacggag atggctcacc
     7921 aggcagccgc cgccggctgc gacctgatcg ctgtctacgg cggcgacggg acgttgatgg
     7981 aggtcatcaa tggcctgatg gcccacggcc accaaacccc tatcgccatt ctgccgggcg
     8041 ggaccggcaa cacggtggcc gccgacctcg gcatcccgca caatctggcc gacgcgctgc
     8101 atgtcgtggc gaccagtcag gggcggcggc aactggacat cggcgaagtc aacggccgct
     8161 atttcctgct gcgggcctat accggcatcg gtagcgacca caacgcctcg cgtgaattga
     8221 aagaccacct gggtatcctg gcctatccta ttaaagggtt gcagttcctg aaggaacagc
     8281 cgccggcagc cttcagaata acggttgaca cagagaagat cgaggagccg ggcgttctct
     8341 gttacgtgaa caacatcgcc tattcgcgcg cgccgcagct acaagactgg ctggagcggg
     8401 tctttctcga cgtgcaggtg cacgacggcg acgagagcag cagcgccgag gaaccgatgt
     8461 tgcagacaat tgacccgacc gatggtctgc tcgatgtggt tctgctgacc cagaaccctt
     8521 tcgaccttca cgccatcaag agttgtgtcg tgcgcagcgg cgaggagcag gcgaccgtcc
     8581 acttcatgca gggcaagcgc atccacatcg acgcggaccc gccgcagacg ctgtggatcg
     8641 atggcgagga atgcggcacg acaccggctt acctgcaggt cgtgccgcag gccattaccg
     8701 tcgtcgttcc cgaaggctaa atgagcgcgt ccttcctgag gttgtttccc aggaaggacg
     8761 cggagctaga gcttgtgcag ggccagcgtc accggcgtgt cgccgatgct gacctgctga
     8821 cgaatgtcat cgttatccgg ttcgccggcc atcagttgtg tggccagagt ttccgcgccg
     8881 atgaagccgc cgaagttctg gaacgtcgcc gcgacatccc cctcggccac gtagtgcagg
     8941 gcgatccggt cggaaacctc caggccggcg tccttgcgca ggctgttgat ggcccggacg
     9001 atgtcgcggg cgtagccctc ttgcaccaac tccggcgtca ggtgcggatc gatggcaacg
     9061 gtgatgccct tgccgccggc gacggccagg ccgccgcgcg actccgtctg caccagtaca
     9121 tcatcgccgt tcagtgtcac catctggccg ttgacggcca actccagccc cgcgcctgct
     9181 tgcaggcgga cggccgcggc ggccgggtcc agttgcgcca gggcagcgcg aacggccggg
     9241 aacagcggcc ccagctttgg ccccagagcg cggttgttgg gcagcaactt gtagctgacc
     9301 agttcgccga cctcggaaac aatgtcgatg gccttgacat tgatctcctc ggccagcacg
     9361 ccggccaact cctgcaagtc ggcccgctcc tgctccgagc cgacgaaaac gcgcgcccgg
     9421 gccagcggtt ggcgcagctt gacatcggcc gagccgcgcg ccgagcgccc caacgcggcc
     9481 gcgtcgatga ccagacgcat cttgtccagc agccgatgat cgagcgcggc cgcgtcggcc
     9541 tgcgggtaga agtggtggtg gacgctcaga agcgggacgc tcaaaaccgg gacgctcagc
     9601 ggcgcgccgg catccccggc gcgcaccaga ttctggtaca tcgcttcggt cgtcaacggg
     9661 agaaatgggg ccagcagttt gataaactca accaggacgt gatagagcgt ggcgtaggcc
     9721 gcggccttgt cggcatcggc ttcgctcttc cagaagcggc ggcgcgaacg gcggacatac
     9781 cagttggaca ggtcatccag caacagctcc agcgccgccg cggcccgctc cgcgtcccag
     9841 gcgtccagcg cttcgcggac gtcaaccgtc gtctcgttga ggcgttcgac gatccagcgg
     9901 tctagttggg aggttccagg ggccaggggc cgggggccag gggaagatgc ttcgcctgtg
     9961 ctcccttgct cggctgccgc ctggggtgtc cagccatccg cgttggcata ggtcaccagg
    10021 aacgagtaga cgttccacag cgggatgagg aagcgccgcc gcgtctcgtc gccgcggtta
    10081 aagccgaatt gcaggttctg ctccggcttg cacgaggcat agagccagcg catcgtgtcc
    10141 gcgcccatct cttcggcggc ctcgttgaac tcgatggagt tgccccacga cttgtgcatg
    10201 gggcggccgt cttcgtccac cagtgtggcg tagccaaaca ggttgtcaaa gggcggctcg
    10261 tcggttagca ccgtgctctg ggccagcagg ctgtagaacc agttgcggaa ctggccgggg
    10321 aaactctccg agatccagtc ggccgggtac cacttctccc agtaggcgcg gtcggtgcga
    10381 tagtgcagcg tgctgaaggc gacgatgccg gcatcgagcc agggattgcc cacgtcggcg
    10441 atgcggctgg tcgtggcccc gcatgagggg caggcaatct tgaccgcgtc gatgtagggc
    10501 cggtgaggtg tatggccggc aaatgtatcc cagccctcca cagcgcgctc ctccaactcg
    10561 tgctcgtcgc ccacgacagt gaacgtgccg cactcctcgc actcccagat aggtagggcc
    10621 agaccatagt agcgcttctt gctgatcatc cagtcgtgca tgttgcgcag ccagtccatc
    10681 tcgcggtcgt agccgaagcc aggcacccag ttgatccgct cgacgcggtc catgatctgg
    10741 tagcgcaggc tggcggtctt ctcttcggcc gttaactgct cacgcggctt gtcgtagacc
    10801 ggccccatgc tgatgaacca ctcatccacc agccgataga ccaactccga gccacagcgc
    10861 cagcagtggg ggtagcggtg ggtgtacttc tgcttgcgat agaagaagcc gccctcgcgc
    10921 agtgcagtga agatgtcctc ggctacgctg ggcgtgttgg gaccgcccag cacttcacgg
    10981 ccggtcagcc agtcgaagcc aggcagatag acgccgttct cgtccagcgg agcgacgacg
    11041 ggcaggtcga actcctggcc cagggcgaag tcctccgcgc cacagccggg ggcgatgtgg
    11101 acgatgcccg tgccctcttc cgcgccaact tccgtccagg ggatgacacg gtggctgtaa
    11161 tccttctcat caaaggcggc gaccacctgc ggcaggtcat cgaacgggcc agtgtactgc
    11221 cagccgagca tgtccgcgcc cttcagccgg ccgacgacct cgttgttctt gcccaccagc
    11281 gagttcttca tggccccttc ggccaggtag tacgtccagc cgtcctcggc ccgcacctgc
    11341 acgtagtcca gttccgggcc gacggcggcg gcgacgttgc tggtcagcgt ccagggcgtc
    11401 gtcgtccaga ccagcagggc ctcgttgggc cggtcgacca gcgggaagcg cacgaagaca
    11461 gaatcgtggg tcacgtcgaa gtagccgtcg gtgacgatct cgtgctggct gatggccgtg
    11521 ccgca
//
LOCUS       DAOSMQ010000027        11496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5606_length_11496_cov_4.286950, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000027 DAOSMQ010000000
VERSION     DAOSMQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5606_length_11496_cov_4.286950"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..969
                     /locus_tag="PK829_01710"
                     /pseudo
     CDS             34..969
                     /locus_tag="PK829_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1031..2137)
                     /locus_tag="PK829_01715"
     CDS             complement(1031..2137)
                     /locus_tag="PK829_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU39960.1"
                     /translation="MESTASLSAKSVARQRLGEWWPYLLVLAFLVLFPAIAALTIGDS
                     TAAQPDAGLFQQLAQRAESGYAKFWQGMLIQMLILGIFAMSYDLLLGYTGILSFGHAM
                     FFGTGGYAIAILIGRNFNWPFGLALIAVVVIALVQSLVFGVLSLRVKGVYLAMVTLAF
                     AEMFFILAEAGDFRAYTGADDGLHGFPVPAYLSATDHRTRFYYLALIFFVAVFLMAKR
                     LVESPTGRVMIASRDNENRATMIGYNVFWFKLIAFVAAGVFAALAGALFALYNVSVTP
                     SVLSVGRTIDALAMTIIGGVGTLVGPILGAAIIQLLGYWLERWFGANWTLAYGILFML
                     IVIFLPYGIVGTARARSFRLKEGWQRWRKLGNRD"
     gene            complement(2309..3715)
                     /locus_tag="PK829_01720"
     CDS             complement(2309..3715)
                     /locus_tag="PK829_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU39961.1"
                     /translation="MSIMLSGATLAALYFLVASGLSLIFGLMDVLNFAHGVLFVLGAY
                     VGLSTFMNPRLLFNTLPFFFVLVAGLIVAQHFGRFFWRRIGAVRSRQVATAVLVLVAA
                     GLVLLALRGFPIRALNAFNITAVGGAVTTAEAQEPMAAMLRRVTMVLLAGLPIGLLLA
                     PKQARDELPLRPVWQTLLIAGVAAVVGYALLLVRDPGETFMLALSADVRFVLAIVLGT
                     VAGALAGVGIETTLIRPFYGNPVTQLVLTLGLSIAGIDLIEAVWGEEGNPPMPPPSLF
                     AANCRSDSLLGWLGEHCQSIDVLGRAFPTYRLFIIAIGIAVFIAVGLLLRRTRIGMII
                     RAGVQDGEMVQALGINVRRVFTFVFALGSALAAMGGVIAAPYLGVFPAMGSLFQLQAF
                     IAVVIGGMGSYVGAAVGTIILGLARAFGDHFVAAGINVPFLDTVIKGSPAIARASTVL
                     IMAIVLLVKPAGIFGKKE"
     gene            complement(3828..4550)
                     /locus_tag="PK829_01725"
     CDS             complement(3828..4550)
                     /locus_tag="PK829_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU39962.1"
                     /translation="MAASPTPTNVLEVRDIHTDIGQFHILDGVSVEVPEGSITVLLGR
                     NGAGKTTTLKSIIGLTPPHSGAVALRGREIQGEPAYRIAARGIGYVPEHRAIFRDLSV
                     EENLRIAERKPGDLSRRGDFIFDLFPDLKRLIKLPGGNLSGGQQQMLAVARALVPENE
                     LLLIDEPSEGLAPVIIEQMMEAIRQLSAHTTVLLVEQNFIMASQLADRYTIIETGRSV
                     KSGRMADLVKDEETITRYLGAT"
     gene            complement(4551..5330)
                     /locus_tag="PK829_01730"
     CDS             complement(4551..5330)
                     /locus_tag="PK829_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU39963.1"
                     /translation="MDDSTILETQDLRKQFGGLVAVDGVSMKVRAHTLHTIIGPNGAG
                     KTTFFNLLSGNIRPSSGRVLFKGEDITGQPAHRRAHLGIGRSFQITNVFPTLTVLENV
                     RLAAQAVGHDSFKFWQHHRHFHQYEERAAAIVAQVGLAAQAGLPARLLPHGGKRKLEL
                     GILLAADPDLLLLDEPTAGMAREEIPMLMEVINGIRAAGDKTIILVEHKMDLVMNVSD
                     RISVMHQGRLLAEGVPMEIAANKTVQAAYLGDLYGDLTERK"
     gene            complement(5494..6942)
                     /locus_tag="PK829_01735"
     CDS             complement(5494..6942)
                     /locus_tag="PK829_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU39964.1"
                     /translation="MKNRRTLWIVLSLFTVFVLALSACGGTTTTETAATEAPAAEPTE
                     AVVEPTEAPAEEPTEAPTEAAVVEPTEAPAEEATVAPTEEAAAAGEQPFAGEELVIGL
                     MTDKSGALAIYGPSQTQGFYLGLEYATDGTMEVAGRPIRVVEMDNGSDPDTAVQQARE
                     LIEAEGAEILIGNVSSSAALATIPVAEENDVIYIAEPAASPAITGENFSPNTFRTSRT
                     SVQDALVMGSGLLGMGDTFVQIAPDNAFGIGSACAFYAIVKGGGGEFVANDTDEGCGT
                     VYAPLETTDFTSYINQILDSGADVLIVSWAGAGFAPMFQQMSQLGVFDEMTVGTGVGD
                     NQTLAAGYADAVGVVGIQVYHYTLPDNEINDWLVQRHMEEYGTPPDLWAAGGMMSAIM
                     AVEGLKATNGDASADALRAVYEDNFSFEGPKGTTTIRAADHVALQTLYFVRVTNVTDP
                     DFKFVELVQEFGPETTVPPCALVDPYADRCAE"
     gene            complement(7130..7936)
                     /locus_tag="PK829_01740"
     CDS             complement(7130..7936)
                     /locus_tag="PK829_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU39965.1"
                     /translation="MPIITTNGIDLYYEIHGRGEPLVLIAGLGYDGWMWHRMIPGLAE
                     HFQVISFDNRGVGQSDKPAGPYTAGMLAADVIGLLDAFDIDKAHIIGHSMGGFIAQAL
                     AIHHLDRVNRLVLSATNFGGPRHAPISPEAMAVLTDVSGDPIARLRRGIVISCAPGFA
                     EQNPALVDDWVDYRVRHPIDPAGYQAQLAIGLSLLPEAAAFENKLGAVTVPTLILFGE
                     HDKVVPPANATLLAEKLPNAQIEILPNAGHFFPFEVPEEANRAIIRFLSA"
     gene            complement(8003..9559)
                     /locus_tag="PK829_01745"
     CDS             complement(8003..9559)
                     /locus_tag="PK829_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU39966.1"
                     /translation="MINELYPLHVGELLSKRAELTPDCAGLVVAESGATYTYRELNGR
                     ANRLANLMQSLGVGKGDRVAILAQNSVVYVDLLYGLAKIGAILAPLNWRLTARELSYI
                     AADCAPRAIVCGPEYAGLLAELRREVDFDIVLGIEGASIEGGLLYEDAVAAAADTEPE
                     QPPLSGEDICCLLYTSGTTGRPKGAMLPHRQVLFNCINTVISWGLSANDISPVMTPMF
                     HAGGLFVFLAPLFYVGGKIVMSRGFDSEASLALIQRERCTIVLGVPTLFQMWMASPSF
                     AAADFSAVRYFVSGGAPCPVSLIHAWREAKGGVLRQGYGLTEVGVNCFTMTDEESVRK
                     VGSVGHPIFHSRMRIVGSDGRDVARGETGELIIWGPHVCAGYWRNEAATAQALRDGWF
                     YTGDMARQDDEGYYYLAGRYKDMIISGGENVYAAEVETVFLEHPAVGEAALIGLPDPQ
                     WGEVGQMIVVTRPGRTTTEEELLSFCRARLARYKVPKRVVFAEALPYSPYGKVIKAEL
                     RQRYGGAGVA"
     gene            complement(9661..10419)
                     /locus_tag="PK829_01750"
     CDS             complement(9661..10419)
                     /locus_tag="PK829_01750"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOU39967.1"
                     /translation="MRLKGKVCLITGGAAGIGRATAERFAEEGATVVIADLNEEAGRA
                     TAEAIGATFYRVNVADRAAVQAWVDDVVAKSGRVDVLVNNAGVLRDSTLVKVKDGELV
                     KQMSEADFDLVIAVNLKGVFNCTQAVAPYMIRQGGGVILNASSIVGLDGNFGQTNYVA
                     TKSAVIGMTKVWARELGRSNIRVNAIAPGFTATEMVAAMPENVLAGMKARTPLGRLGE
                     PRDIANAYLFLASDEASFITGEVLRVDGGIVVGT"
     gene            complement(10515..11327)
                     /locus_tag="PK829_01755"
     CDS             complement(10515..11327)
                     /locus_tag="PK829_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU39968.1"
                     /translation="MSMVNVNGVSLYYELHGPAGAPVLVLNNGILMNAAGSWVFQTAA
                     LSRHYRLLQYDCRGQGQSDHPAGPYTMALHAADLVALLDALGSEHTDTTRVHIAGASY
                     GGEVAQAFALAYPERVRSLILMDTASELGPELRATAENWLAAARRADPEEFYNATVPW
                     NFSPPFIREHEALLADARRRYALLDFPAVARLCECFLGVDFTARLGEITAPTCIIVGA
                     LDLLKGPAYAHMLKAAIPQAELHVLPGAGHASPWERPEEFNTVVLGFLAKQP"
BASE COUNT         2131 a         3674 c         3788 g         1903 t
ORIGIN      
        1 aggaagcctg cgggaattgt tcgaacctga ggagtgcaga tgattgccac ccagtgggcc
       61 acgctgcaaa gcgatgtcgc cggggcagcg cgcatctttg gcctgctgga cgagccggcc
      121 gatatcatcg acaagccggg cgcggtcgct atcccgtcca tcaacggcca tgtggtctat
      181 caagacgtct gggcggagta caatcccggc gagccagtcc tgcgtggcgt ggacatcgag
      241 gcccaggcgg gccagaccgt ggccatcgtc ggccctacgg gcgcgggcaa aacgacaatc
      301 atcaatctgt tgccgcgctt ctgggacgtg ggcaccgcag acggtgctgg cggagccatc
      361 acgatcgacg gctacgacct gcgcgacgtg acccaacaga gcctgcggca gcagatcggc
      421 atcgtgttgc aggacacttt cctgttcagc gacacggtga tgaacaacat ccgctacgga
      481 cggccggagg ccaccgacga ggaagtcatc gccgcggcgc agttgtccca cgcgcatgag
      541 ttcattgagc gcctgcccga cggctatcag accgttctgg gcgagcgcgg cgccggcctc
      601 agccaggggc agcgccagct cctggctatc gctcgcgtgg ccctcatcga cccgcggatc
      661 ctcatcctcg acgaggctac cagcagcgtc gacacgcgca ccgagcaaca gattcagcgg
      721 gcgctggaga agttgctgca cggccgcacc agcttcgtca tcgcccaccg cctgagtacc
      781 atccgtcacg ccgaccaggt gctgttcgtc gacgccggcc aggtcattga gcgcggtacg
      841 cacgacagcc ttctggccca aaacgggcgg tacgccgagc tctacatgag tcagttccgg
      901 cgcaagggtg ctgagactga ggaagaggaa gcggccgagg ccgagacata cctcttcccc
      961 ggcgactaga ccaaagtagg ggccaggagc cagggaagca cactcttccc tagcccctgg
     1021 cccctattcc ctaatcccta tttcctaatt tccgccagcg ctgccagcct tctttcagcc
     1081 ggaacgagcg cgcccgcgcg gtgccgacga tgccgtaggg caggaagatg acgatgagca
     1141 tgaagaggat gccgtaggcc agcgtccagt tcgcgccgaa ccagcgctcc agccagtagc
     1201 caagtagctg gatgatggcc gcgccgagga ttggcccgac cagtgtgccc acgccgccga
     1261 tgatggtcat ggccagggcg tcgatggtac ggccgacact cagcaccgag ggcgtgacgc
     1321 tgacgttata gagggcgaag agcgccccgg ccagcgcggc aaagacgccc gcggccacaa
     1381 aggcgatcag cttgaaccag aagacattgt agccgatcat cgtcgcccgg ttctcgttgt
     1441 cccggctggc gatcatgact cgccccgtgg gcgactcgac cagccgcttg gccatcagga
     1501 acacagccac gaagaagatc agggccagat agtaaaagcg ggtgcggtga tcggtggcgc
     1561 tcaggtaggc gggcacgggg aagccgtgca ggccgtcgtc cgcgccggtg taggcacgga
     1621 agtcgcccgc ctcggccagg atgaaaaaca tctcggcaaa ggccaacgtc accatagcca
     1681 ggtagacccc tttaacacgc aaggacagca ccccgaagac aaggctctgc accagagcaa
     1741 tgacgaccac ggcgatgagc gctagaccga acggccagtt gaagttgcgg ccgatgagga
     1801 tggcgatggc atagccgccc gtgccgaaga acatggcgtg gccaaaagat agaatgccgg
     1861 tgtagcccag cagcagatca tagctcatgg cgaagatacc caggatgagc atctggatga
     1921 gcatgccctg ccagaacttg gcataaccgc tctcggcgcg ctgggccaat tgctggaaca
     1981 ggcccgcgtc aggctgcgcg gccgtcgaat cgccaatggt cagcgcggcg atggccggaa
     2041 agagcaccag gaaggccagg accagcagat agggccacca ctcgcccagc cgttggcgag
     2101 ccacactctt agctgatagg gaagcagtac tttccatacg tgatcaccag ttctgtttcg
     2161 cctcacaggc cctcccctaa cccttcccag agggcgtggg attgtctggc cccctctccc
     2221 cttggtagag ggctggggag agggtcttaa ccgcgcccct cccttaccct tcccagaggg
     2281 cggagaatgg gtggagagcg ggttctcttt actctttctt gccgaagatg cccgccggct
     2341 tgaccagcaa gacaatggcc atgatcagca ccgtcgaggc acgggcgatg gccgggctgc
     2401 ccttaatgac cgtatccaga aacggcacat tgatgcccgc ggcaacgaag tggtcgccaa
     2461 aggcgcgggc caggcccagg atgatcgtgc ccacggccgc gccgacgtag ctgcccatac
     2521 cgccgatgac gacggcgatg aaggcctgta actggaacag cgaccccatc gctgggaaga
     2581 cgcccaagta tggtgcagcg atgacaccgc ccatcgccgc cagcgccgag ccgagggcaa
     2641 agacaaaggt gaacactcgg cgcacgttga tgcccagcgc ctgcaccatc tcgccgtcct
     2701 gcacgccagc gcggatgatc atgccgatgc gcgtgcggcg caatagaaga cccacggcga
     2761 taaagacggc gatgccgatc gcgatgatga acaggcgata ggtggggaag gcgcggccga
     2821 gcacgtcaat cgactggcaa tgctcaccga gccagcccag gagcgaatca gagcggcagt
     2881 tggcggcaaa gagcgacggc ggcggcatgg gcgggttgcc ctcttcgccc cagacggcct
     2941 cgatgaggtc gatgccggcg atgctcaggc ccaacgtcaa taccagttgc gtcaccggat
     3001 tgccatagaa cggccggatg agggtcgtct cgatgccgac gccggccagc gcgccggcga
     3061 ccgtgcccag cacgatggcc aggacaaagc gcacatcggc tgacagggcc agcatgaagg
     3121 tctcgccggg gtcgcggacg agcagaaggg catagccgac gaccgcggcc acgccggcga
     3181 tgagcagcgt ctgccagacc ggccgcagcg gcaactcatc tctggcctgc ttgggggcca
     3241 gcagcaagcc gatgggcagg ccggccagca gcaccattgt cacgcggcgc aacattgccg
     3301 ccattggctc ctgcgcctcg gccgtggtca ctgcgccgcc gacagcggtg atgttgaagg
     3361 cgttgagggc gcgaatgggg aagccgcgca gggccagcaa aaccaggccg gccgccacca
     3421 gcaccaggac ggccgtggcc acctgccggc tacgaacagc gccaatgcgc cgccagaaaa
     3481 agcggccgaa gtgctgggcc acgatcaggc cggcgacaag gacgaagaag aagggcagcg
     3541 tgttgaacag caggcgcggg ttcatgaacg tggacaggcc aacgtaggcc cccagcacaa
     3601 acagcacgcc gtgggcgaag ttcagcacgt ccatcagacc gaagatgagc gataggccgg
     3661 aggcgaccag aaagtagagc gccgccagcg tagccccgga cagcatgatc gacatccaga
     3721 ctttgggttc aaggctctgg agcaggccgg ccatgagcac gacgacgatg gcagcgatga
     3781 tcagcaggcc ccggttgttc ttccacagtg cggtcatagc ggctccgtca ggtggctccc
     3841 agatagcggg tgatcgtctc ttcgtccttg accaggtcgg ccatgcggcc gcttttcacc
     3901 gaccggcccg tctcgatgat ggtgtaccgg tcggccaact ggctggccat gatgaagttt
     3961 tgctccacca gcagcacggt cgtgtgggct gagagctggc gaatggcttc catcatctgc
     4021 tcgataatga ccggggccag cccctcgctc ggctcgtcga tgagcagcag ttcgttctct
     4081 ggcaccagcg cgcgggcgac ggccagcatc tgctgttgcc cgccagacag gttaccgccg
     4141 ggcagcttga ttagccgctt caggtcaggg aagaggtcaa agatgaagtc accgcggcga
     4201 ctcaggtcgc ccggcttgcg ctcagcgatg cgcaggtttt cctcaaccga caggtcgcgg
     4261 aagatggcgc ggtgctccgg cacatagccg atgccgcgcg cggcgatgcg ataggccggc
     4321 tcgccctgaa tttcccggcc gcgcagagct accgcgccgc tgtggggtgg ggtcaggccg
     4381 atgatgcttt tgagcgtcgt cgtcttgccc gcgccgttgc ggcccagcag cacggtgata
     4441 ctgccttcgg gcacctcgac cgacacgccg tccaggatat ggaattggcc gatgtcggtg
     4501 tggatgtctc tgacttcgag aacgttggtg ggcgtgggcg aagcggccat ttatttcctt
     4561 tcggtgagat cgccgtacag gtcgcccagg taggccgcct gcacggtttt gttggcggcg
     4621 atctccatcg gcacgccctc agccaggagc cggccctggt gcatgaccga aatgcggtcg
     4681 gagacgttca tcaccaggtc catcttgtgc tcgacgagga taatggtctt gtcgccggcg
     4741 gcgcggatgc cgttgataac ctccatcagc atgggaatct cttcgcgggc catgccggcc
     4801 gtcggctcgt cgagcagcag cagatcgggg tcggcggcca acaggatgcc cagctctagc
     4861 ttgcgcttgc cgccatgggg caacagccgc gcgggcaggc cggcctgcgc ggccaggccc
     4921 acctgggcga cgatggctgc ggcgcgctcc tcgtactgat gaaagtggcg gtggtgttgc
     4981 cagaacttga agctatcgtg gcccacagcc tgggcggcca gacgcacgtt ctccagcaca
     5041 gtcagggtgg gaaagacgtt ggtgatctgg aacgagcgac cgatgcccag gtgcgcgcgg
     5101 cggtgggccg gctggccggt gatgtcttcg cccttgaaga gcacccgccc actgctgggc
     5161 cggatgttgc cactgagcag gttgaagaag gtcgtcttgc ccgccccgtt cggcccgatg
     5221 atggtgtgca gcgtatgggc gcgcaccttc atgctgaccc catccaccgc caccaggccg
     5281 ccgaattgct tgcgcaagtc ctgggtttcc agaatggtcg aatcgtccat agtgaaacag
     5341 ctcacgcgaa ggcgcaaagg acgctaaaag gaacctaaga agcccacgcc aagacgcggc
     5401 ggcgcaaagg gcgcaaagtc aacctcaatc tcttccttgg cgttctttgc ccctttgcgc
     5461 ctttgcgtga gcgttcgtta catcaagtca atcctactcg gcgcagcgat cggcgtaggg
     5521 atcgacgaga gcgcagggcg gcaccgtggt ctccgggcca aactcctgca ccaactcgac
     5581 gaacttgaaa tccgggtcgg tgacgttggt cacgcgcaca aagtagagcg tctgcaaggc
     5641 cacgtgatcc gcggcgcgga tggtcgtcgt gccctttggt ccctcaaagg agaagttgtc
     5701 ctcgtagacg gcgcgcagcg cgtcggcgct ggcgtcgccg ttggtcgctt tcaggccctc
     5761 aaccgccatg atggccgaca tcatgccacc cgcggcccac aggtccggcg gcgtgccata
     5821 ctcttccatg tgccgttgga cgagccagtc gttgatctcg ttgtccggca gcgtgtagtg
     5881 gtagacctgg atgccgacca cgccgacagc gtcggcgtag cccgcggcca acgtctggtt
     5941 gtcgcccacg cccgtgccga cggtcatctc atcaaagacg cccagctggc tcatctgctg
     6001 gaacatcggc gcgaagccgg cgccggccca ggagacgatc agcacgtcgg cacccgaatc
     6061 gagaatctga ttgatgtagg acgtgaagtc ggtcgtctcc agcggcgcgt agaccgtgcc
     6121 gcaaccctcg tcggtgtcgt tggcgacgaa ctcgccgccg ccgcccttaa cgatggcgta
     6181 gaaggcgcag gccgagccga tgccgaaggc gttgtccggg gcgatctgca cgaacgtgtc
     6241 gcccatgccc agaagaccac tacccatgac gagcgcgtcc tggacgctgg tgcggctggt
     6301 gcggaaggtg ttggggctga agttctcgcc cgtgatcgcc ggcgacgccg ccggttcggc
     6361 gatgtagata acgtcgttct cctcagcgac cgggatagtg gccagggcgg cgctggagct
     6421 gacgttgcca atcagaattt cggcgccttc agcctcgatg agttcacggg cctgctggac
     6481 ggccgtgtcc ggatcgctgc cgttgtccat ttcgacaacg cgaatgggcc ggccggcgac
     6541 ttccatggtg ccgtcggtgg cgtactccag gcccaggtag aagccctgcg tctggctggg
     6601 gccatagatg gccaacgcgc ccgacttgtc ggtcatcagg ccgatgacca gttcttcgcc
     6661 ggcgaagggc tgctcgcccg cggcggcggc ctcttcggtc ggcgcgacgg tagcttcctc
     6721 agcgggagct tcggttggct caacgacggc ggcctcggtg ggagcttcgg tcggttcctc
     6781 agcgggggct tcggttggct caacgacggc ttcggtcggc tcggccgccg gggcctcggt
     6841 cgcggccgtt tccgtggtgg tcgtgccgcc acaggcgctc agggccagca cgaagacggt
     6901 gaacagggac aaaacgatcc acagcgttcg acggttcttc atctatctcc tccaatctaa
     6961 gtattcaaca gcaaacagat catcgaaagc atctgggcag aggactgccg gacagcagac
     7021 tgccccttct ttatagccta gcacccaccg gttactgttc ggttacagcc cggcggctgg
     7081 cgtggtaggg gcgagtctga gactcgcgcc taccacgcac tcctatacat caggccgaca
     7141 ggaagcggat gatggcccgg ttggcctcct ccggaacttc aaacgggaag aagtggcccg
     7201 cgttgggcag aatctcgatc tgcgcgttgg gcagcttctc agccagcagc gtggcgttgg
     7261 ccggcggcac gaccttgtcg tgctcgccaa acaggatgag cgttggcaca gttaccgcgc
     7321 ccagcttgtt ctcgaacgcg gccgcctccg gcaacagcga caaaccaatg gccaactgcg
     7381 cctgatagcc ggccgggtcg atggggtggc ggacgcgata atcaacccag tcatcgacga
     7441 gagcggggtt ctgctcggca aagccgggcg cgcaactgat gacgatgccg cgccgcagcc
     7501 gggcgatggg atcgccgctg acgtcggtca gcacagccat cgcctccggg ctgatgggcg
     7561 cgtggcgcgg cccgccgaag ttggtggccg agagaaccag ccggttaacc cggtcgaggt
     7621 ggtggatggc cagcgcctgg gcgatgaagc cgcccatcga gtggccgatg atgtgggctt
     7681 tgtcgatgtc gaatgcgtcc agcaagccga tgacgtcggc ggctaacatc ccggcggtgt
     7741 atggcccggc cggcttgtcg ctctggccca cgccgcggtt gtcgaagctg atcacctgga
     7801 agtgttcggc caggccgggg atcatgcggt gccacatcca gccgtcgtag cccaggccgg
     7861 caatgagcac cagtggctcg ccgcggccgt ggatttcgta gtagagatcg attccgttcg
     7921 tggtaatgat aggcatattg gctccgatat tggttggtgg gtataggcgt gccgtagggg
     7981 cgggttggcg ggttgtagac cgctatgcca cacctgcgcc gccgtagcgc tggcgcaact
     8041 ccgccttgat caccttgccg tagggcgaat agggcagcgc ctcggcaaag acgacgcgct
     8101 tgggcacctt gtagcgcgcc aggcgggcgc ggcagaagct cagcaactct tcttcggtgg
     8161 tcgtgcggcc cggccgagtg acgacgatca tctggccaac ttctccccac tgggggtcgg
     8221 gcaggccgat gagggccgcc tcgcccacgg ccgggtgctc caggaaaaca gtctccacct
     8281 cggcggcgta gacgttctcg ccgccgctga tgatcatgtc cttgtagcgg ccggccagat
     8341 agtagtagcc ctcatcgtcc tggcgggcca tgtcgccggt gtagaaccag ccatcgcgca
     8401 acgcctgggc cgtggccgcc tcgttgcgcc agtagccggc gcagacgtgc ggcccccaga
     8461 tgatcagctc gcccgtctcg ccgcgggcca cgtcgcgccc gtcgctgccg acaatgcgca
     8521 tgcggctgtg gaagatgggg tggccgacgg agccgacctt gcgcaccgac tcttcatcgg
     8581 tcatggtaaa gcagttgacg ccgacctcgg tcaatccata gccctgacgc aacacgcccc
     8641 ccttggcctc gcgccaggcg tggatgagcg acaccgggca gggcgcgccg ccgctgacga
     8701 agtagcgcac ggcgctgaag tcagccgcgg cgaaggacgg gctggccatc cacatctgaa
     8761 acagcgtcgg cacgcccaga acaatggtgc agcgctcgcg ctggatgagg gccagcgacg
     8821 cctcgctgtc gaagccgcgg gacatgacga tcttgccgcc aacgtagaac agcggcgcta
     8881 gaaagacgaa cagcccgccg gcgtggaaca tcggcgtcat cacgggcgag atgtcgttgg
     8941 cgctgaggcc ccaactgatg accgtgttga tgcagttgaa cagcacctgg cggtggggca
     9001 gcatggctcc cttggggcga ccagtcgtgc cggaggtgta gagaaggcag cagatgtcct
     9061 cgccgctcag gggcggctgc tccggctcgg tgtcggccgc ggcagcgacg gcgtcctcat
     9121 acaatagccc gccctcaatc gacgcgccct cgatgcccag gacgatgtcg aaatccacct
     9181 cgcggcgcaa ctcggccagc aagccggcat actccgggcc gcagacaatg gccctgggag
     9241 cgcagtcggc ggcaatgtag ctcaattcgc gcgccgtcag ccgccagttg agcggcgcga
     9301 ggatagcgcc gatcttggcc agcccgtaga gcaagtccac atagacgacg ctattctgcg
     9361 ccaggatggc gacgcggtcg cccttgccca cccccagcga ttgcatcagg ttggccaggc
     9421 ggttggcgcg gccgttcaat tcgcggtagg tataagtcgc gcccgactcg gccaccacca
     9481 ggccggcgca atccggcgtc aactcggcgc gcttgctcag cagctcgccg acgtgcaatg
     9541 ggtaaagctc gttgatcatt gtagggttag gttctagggt ctaggttcca ggggccaggg
     9601 tctagaggcc agggaagagc gttcttcccc tagccctagc ccctggctcc taatccctac
     9661 tcacgtcccg acaacgatgc cgccatccac gcgcaagact tcgccggtga tgaacgacgc
     9721 ctcgtcggag gccagaaaca ggtaggcatt ggcaatgtcg cgcggctctc ccaaccgccc
     9781 cagaggtgtc cgcgccttca tgcccgccag cacgttctcc ggcatcgccg cgaccatctc
     9841 cgtagccgtg aagccagggg cgatggcgtt gacgcgaatg ttggagcgcc ccagttcgcg
     9901 cgcccagact ttggtcatgc cgatgacggc cgacttggtc gccacatagt tggtctggcc
     9961 aaagttgcca tccaggccaa cgatggacga ggcgttaaga atcacgccgc cgccttgccg
    10021 aatcatgtat ggagccacgg cctgggtgca gttgaagacg cccttcaggt tgacggcaat
    10081 aaccaggtcg aagtcggcct ccgacatctg tttgaccagt tcaccgtcct tcactttgac
    10141 cagggtgctg tcgcgcagaa cgccggcgtt gttgactagc acatcgaccc ggccggattt
    10201 ggcgacgaca tcatcgaccc aggcctggac ggcggcgcgg tcggcgacgt tgacgcggta
    10261 gaacgtcgcg ccgatagcct cggctgttgc ccggccggcc tcttcgttca ggtcggcgat
    10321 gacgacggtc gcgccttcct cggcgaagcg ctcggccgtc gcccggccga tgccggccgc
    10381 cccgccggtg atcagacaca ctttcccttt caaacgcata gtttccttcc tcgacagata
    10441 actgcccatt gacaatggta tagggccgtc cgggtagggg cgggtctcgg acctgcccct
    10501 acaggccccg ctggttacgg ctgtttggcc agaaacccca gcacgacggt attgaactcc
    10561 tctgggcgct cccacggcga ggcatggccc gcgccgggca ggacatgaag ctccgcctgg
    10621 gggatggccg ccttgagcat atgggcatag gccggcccct tcagcaggtc gagcgcgccg
    10681 acgatgatgc aggttggggc cgtaatctcc ccgagccggg cggtgaaatc gacgcccagg
    10741 aagcattcgc acaaccgggc cacggctggg aagtcgagca gggcgtagcg gcggcgagcg
    10801 tcggccagca gagcctcgtg ctcgcgaatg aacggcggcg agaagttcca cggcacggtg
    10861 gcgttgtaga attcttctgg gtcggcgcgg cgggcggcgg ccaaccagtt ttcggccgtg
    10921 gcccgcagct ccggccccag ctcgctggcc gtgtccatca agatcaggct gcgcacgcgc
    10981 tccgggtagg ccagggcgaa tgcctgggcc acctcgccgc cataggaggc cccggcgatg
    11041 tggacgcggg tggtgtcggt gtgttcgctg cccagcgcgt cgagcagcgc aaccaggtcg
    11101 gcggcgtgga gagccatcgt gtaggggccg gcagggtgat ctgactgtcc ctgcccgcgg
    11161 cagtcgtatt gcagcaggcg gtagtggcgc gacagcgccg ccgtttggaa aacccacgag
    11221 ccggcggcgt tcatcaggat gccgttgttg agcaccagca ccggcgcgcc ggccgggccg
    11281 tggagttcat agtagagact gacgccgttg acgttaacca ttgacataac gacaccttat
    11341 aaagcccgct ccccggcccg cgggatcaga tcacccaccc acaccagcgc ctccccgtcg
    11401 cagaccgtcg cgccggccgc ggtaatccac gtccgcaggt tgacaagctc cttgtccggc
    11461 cgcaggcgaa tgatctccac gtgggccgcc agctcg
//
LOCUS       DAOSMQ010000028        11397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5671_length_11397_cov_4.864486, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000028 DAOSMQ010000000
VERSION     DAOSMQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5671_length_11397_cov_4.864486"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..975
                     /locus_tag="PK829_01760"
     CDS             43..975
                     /locus_tag="PK829_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU39969.1"
                     /translation="MTNPLSVAGILITLAFAFTALFAPVLAPPPNENWPRMQIPRDGF
                     RAEPQPPMSEWNKNVPMRVPWWYRNVWPRQESWVHIMGTTSGQYDIWYGVVWGARTAF
                     FASFVVTTLTTILGVIVGALAGFYGGWVDELLMRVVEIFVAFPFITGALILASLLTPR
                     LGRSIWPATIALVIFGWTGYARQLRGDILATKERDYVLAARASGAKDSHLMIKHVIPN
                     AMFPTLVRISLNMGSVVLSFAALSFLGIGLPEGFADWGQVVSFARSFIISLDTYWYII
                     VFPGVALLLYGLGWNLIGDALRDILDPKLRGKKA"
     gene            1088..1894
                     /locus_tag="PK829_01765"
     CDS             1088..1894
                     /locus_tag="PK829_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39970.1"
                     /translation="MTRAIRRWAVVVVLLGLGLTALFATAAAQIERGGPKLPTPTPAP
                     TVPGAVPTMDRLAPPPTVAVPNQADEGAQLYWLHCQPCHGDVGQGLTDDWRAQYPPED
                     QYCWNSGCHGPQPYDQDFALPTAVPAIIGEGALTKFPTLDSVYRYVSVAMPYFFPGDL
                     TQEEYLAILAHLARGNGLWDGRTLTLDTLPQYAIPGAVAGAATPTLPAVAQTTPAPST
                     PAPTSAALATGQPAHTGGMGAAGRLVLVGGVVVLFALGGFVLWLRRSRSS"
     gene            1867..3009
                     /locus_tag="PK829_01770"
     CDS             1867..3009
                     /locus_tag="PK829_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39971.1"
                     /translation="MAETQPLQLDLDAPDMHTLPAYRRLGLLNGLLIGLALGLGAWGV
                     EVVRAARLPLPLTVPALLLGILLLTILGAVIGWLSARIARTWVTVLLWLAGAVVASLG
                     IGYLPFHGRTVTAWLADPRFFGRNVYPNSLGGSNLGLVLSGLFILVVLGALGLLQSYR
                     LENMAGEMHNRRWPSRRAWLALLLPLPLVFVSALSTSSGMVNPAATAAGLANHAILRA
                     QAYEGDLRDLPNEAGISYLGLRPVHDLIAGPFTLGFVDNNVATATATVAAHFDSGNWI
                     HCSIINDQFNFCYDARLVYVDGLRGLITGEAPPENCHTCDLTATDEAAAWLAEHSAAF
                     GPQPVVTLEAQQGNTVLLRAAGEDGFAALCWVEGIDPPTVTSCAEE"
     gene            3048..4115
                     /locus_tag="PK829_01775"
     CDS             3048..4115
                     /locus_tag="PK829_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU39972.1"
                     /translation="MPPFVKQLLLRLLALPLTLLIITAALYGIIMLAPAEERAQLYLP
                     PRTPPLTQEESQRLMARLVTEHGLDDPYPVQYARWLGDLLRGDWGWSPTFKADVLTLL
                     KKRLPVTAELAFYAVLVIVPTALIGGVLSGWRANGRFDRGYRLATFVATSIPPFILGL
                     FLLSIFYVGLGWFPPGRTGIYELSLSTSDFRAITGFLTFDGLLNGRLDVVADALRRLV
                     LPVFSLSLLYWATVGRVTRVAMIEEQTRDYLLTARAKGLRERRLEWRHAFPNAVGPAL
                     TAMILSAAGLVTGIYIVESVFGFKGLSELVTKGFFVAPDAPLVMGFAVFSVLLVLPII
                     LLFDLLRVTLDPRLREDGLEL"
     gene            4120..5100
                     /locus_tag="PK829_01780"
     CDS             4120..5100
                     /locus_tag="PK829_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU39973.1"
                     /translation="MRRRLRPFRRRSTLIGLALVLGFCLLAALAPVLAPPLNPADPWP
                     FMNVSQSFSRQPQPPGPGLPLGTTPQIQNMPQYGLAPGQDASYQWDVFYTLVWGARSA
                     LRFGLLVTILTALIGIAVGVVSGYAGGLSNLLAMRVTDAFLAFPVIAGVWVIQRAFYG
                     NIYNPFNDPTTLTGWEAFLYRARIDPIMIAFILFSWMPYARLMNAQARRVKNMDYIRA
                     AEAMGATGGRVIWRHLLPNTIAPAIVLAARDVGGMVILASAFIFIGFGGNVAWGTMLV
                     SSRDFVIGLNGNPFAYWWTFVPISLALVLFGVGWNLLGDGLNAALNPHGR"
     gene            complement(5104..5379)
                     /locus_tag="PK829_01785"
     CDS             complement(5104..5379)
                     /locus_tag="PK829_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOU39974.1"
                     /translation="MVTAGPWRLVFTKQAQRDAKRLTATGLRPRAEALLAVLRENPYQ
                     TPPPFEKLVGDLSGAYSRRINIQRRLVYQILDDERTVKVLRLWPPYE"
     gene            complement(5373..5615)
                     /locus_tag="PK829_01790"
     CDS             complement(5373..5615)
                     /locus_tag="PK829_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOU39975.1"
                     /translation="MTTLSATEARTNLYKLLNQVSETHEPVLITGKRGNAVLVAEEDW
                     RAIEETLYLLGIPGMRESIIDGMATPVDECDTEIEW"
     gene            complement(5669..6700)
                     /locus_tag="PK829_01795"
     CDS             complement(5669..6700)
                     /locus_tag="PK829_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOU39976.1"
                     /translation="MDDQLLTALAEACAPFGDGRFHVCELTIAPGDGRVCLQGRVLDT
                     ATRDAVLARLRQELPDVRWDAEGVDVLRQARPQRLTVATNLTGLQRSPSWLGEQQSQP
                     RAGAAVEVLAEDERWVFVRLDDGYLGWMYRAYLSEAAAPSPTHQVAAPFTLLYAGPNY
                     LAPVVTRLFAGTPVAVETQDNGWARLASAAGEGYADPMDLRPLADDRPLDIRRRHMVH
                     HDALQFIGVPYLWGGTSVHGIDCSGFAQLLHQLAGVVTPRDADQQFAAGRPVEPPFAP
                     GDLFYFGAPGDHRDVSHVGVSMGPEVEPAGWGMIHSSRGRNGVYVDNVQAVASLRERF
                     LGARRFLSE"
     assembly_gap    6805..6904
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6910..7941)
                     /locus_tag="PK829_01800"
     CDS             complement(6910..7941)
                     /locus_tag="PK829_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOU39977.1"
                     /translation="MTRLTWEPITLRLRTPFRVSYGASETRRAHWLRLAGDEGWGEGT
                     IPPYYGISDEAMAAVWAAADRLAAPFPDDPAGIPAWLAAEPAIAAGPAPARAALDLAL
                     HDRIGRRLGQPLHALLGLPVPRPLPTSFTLGIDAPEVMAEQAAALHDCSVIKLKLGGD
                     DGGDVARVAAVRAARPDVTLYVDANAGWLPDDAIAQIERMLPYGVALVEQPVAKGDIA
                     GMGRVQAACAVPVVADESLQSLADVDRLAAAGVRGVNLKLMKLGGLAAGLAVLRRARA
                     LGLGVMLGCMVETAVGATAMAHLMGLADWLDLDAPWLVADDPFDGLTYDDAGRVHVPD
                     RPGIGVIRR"
     gene            complement(7968..9113)
                     /locus_tag="PK829_01805"
     CDS             complement(7968..9113)
                     /locus_tag="PK829_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOU39978.1"
                     /translation="MVSSTQTILDHIRGQRADMVALLADLVAAESPSDAPETQAGPIG
                     ILYEQFDRLGYHVRHTPGARTGGYLIARHPAAGERPTQLLLGHCDTVWAVGTLAEMPL
                     EVQDDVLRGPGSYDMKAGLAQGLFALRALHELGLEPPLAPVFLINTDEEIGSIESRPV
                     IEQLARESARALVLEPAAGPDGALKTARKGTGDFEIVVHGRAAHAGLEPEKGINAIVG
                     MAEIVRTLATLNDYPRGVSVSVGLISGGLRTNIIPPECRAVVDVRAVTVVDAERLEQA
                     IRVLPAPLPGTTVTVSGGFDRAPLERTPRNAALWELARDVGAEMGLALTETAVGGGSD
                     GNLTSPFTATLDGLGPVGDGAHARHEHVIISKMVERSALLAALLMRA"
     gene            complement(9269..10840)
                     /locus_tag="PK829_01810"
     CDS             complement(9269..10840)
                     /locus_tag="PK829_01810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HOU39979.1"
                     /translation="MSILYAITVALMLLVPVGVAVALRRRFRAPWLLWSAGAVAFFLS
                     QAYHLPLNNWLADIGLIGPVAAGAPDLLRTAVVLGLSAGVCEGLMRAAAFWLLHRRGL
                     VGRWSGGVMVGLGHGGLEAMILGAFTALSVAGLFALRGTDLTALNLPAAQLAALEAQL
                     AAVATPSAAFLLPLVERLLAMALHVAISLLFWLAFRRRNVLYAIAGVLYHALVDGALV
                     YIGPAVSNAWLLEGLVALLVVPGLALIGWTYRRYGVAEPPHRPRPLREEWAQYAALLR
                     KELLEQWRTRRALVVGVVFILFGLTSPLLARFTADLLRSVPGAEQFSGLVPEPKTADA
                     VTQYVKNLTQFGFIIAILLGMGAIAGERERGTAAMTLSKPIPRWAFVLAKFDAQALVY
                     LAALLLAGLGAWFYTGQLFEPGLALGPFMAGNVFLWLWLLAFVAVVLLGSALGRTTLL
                     AAGLGLLGCVIVLVGGAFPQAAALLPAGLVAWISQLGIPDPQPVNGWGALAGSLVLIA
                     FCLITAVGVVERQEV"
     gene            complement(10837..>11397)
                     /locus_tag="PK829_01815"
     CDS             complement(10837..>11397)
                     /locus_tag="PK829_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU39980.1"
                     /translation="GAARRRVGGFSGGMKQRLGLAQALIHRPGVLILDEPMAGLDPAG
                     RRDVLDLLEALRAHVTVFFSTHILSDVERVCDRIGILHEGRLIEVAGREELLSRYTSD
                     VAELVVPEETAGRLPAFAAALAAEPWVAGVTTEANRVRVAAADAAAGQAALLPLVVAH
                     NLPLLRYEWVRPNLEEIFLSLSEEAA"
BASE COUNT         1722 a         3965 c         3842 g         1768 t
ORIGIN      
        1 aaggccgtcg gcccggaatg ggcgcggctc atccgcggca tcatcaccaa cccgctctcc
       61 gtggccggca tcctcatcac cctggcgttt gccttcacgg ccctcttcgc gcccgtgctg
      121 gccccgccgc ccaacgagaa ctggccgcgc atgcagatcc cccgcgacgg cttccgcgcc
      181 gagccgcagc cgcccatgag cgaatggaac aagaacgtgc ccatgcgcgt cccgtggtgg
      241 tatcgcaacg tctggccgcg ccaggagtcg tgggtgcaca tcatgggcac gaccagcggc
      301 cagtatgaca tttggtatgg cgtcgtctgg ggcgcgcgca cggccttctt cgccagcttt
      361 gtggtgacga cgttgacgac gatcctgggg gtgatcgtcg gcgcgctggc gggcttctat
      421 ggcggctggg tcgatgagct tctgatgcgc gtcgtggaga tcttcgtcgc cttcccgttc
      481 atcaccggcg cgctcatcct ggcctcgctg ctgacgccgc gactcggccg aagtatctgg
      541 ccggccacca tcgccctggt catcttcggc tggacgggct acgcccgcca gctgcgcggc
      601 gacatcctgg ccaccaagga gcgcgactac gtgctggccg cgcgcgccag cggggccaag
      661 gactcgcacc tgatgatcaa gcacgtcatc cccaacgcca tgttcccgac gctggtgcgt
      721 atttcgctca acatgggttc ggtggtgctg tcgtttgccg cgctcagctt tttgggcatt
      781 ggcctgccgg aaggcttcgc cgactggggc caggttgtca gcttcgcgcg gtcgttcatc
      841 atctcgctcg acacctactg gtacatcatc gtcttccccg gcgtggcgtt gctgctctac
      901 gggttgggct ggaacctcat cggcgacgcc ctgcgcgaca tcctcgaccc gaagttgcgc
      961 ggcaagaagg cataagaagg ggacagtgac cagtgaccag taggtcagtg ggtcagtagg
     1021 ccagtgggcc agtgggcacg gaggcgggta gacgacaagt tgcccgcctc tttatttttg
     1081 agccgtgatg acaagagcaa tccgacggtg ggccgtggtg gtggtgctgc tgggcctggg
     1141 gctgacggcg ctattcgcca cggccgcggc ccagatcgag cgcggcggcc ccaaactgcc
     1201 aacgcccacg cccgcgccga ccgtgcccgg cgcggtgccg acgatggatc ggctggcccc
     1261 gccgccgacc gtggccgtcc ccaaccaggc cgacgagggc gcgcagctct actggctcca
     1321 ctgccaaccc tgccacggcg acgtcggcca gggcctgacc gacgactggc gcgcccagta
     1381 cccacccgaa gaccagtact gctggaatag cggctgccac ggcccgcagc cctacgacca
     1441 ggacttcgct ctgcccacag ccgtgccggc tatcatcggc gagggcgcgc tgaccaaatt
     1501 ccccacgctc gacagcgtct atcgctacgt cagcgtggcc atgccctact tcttccccgg
     1561 cgacctgacc caggaggaat atctggccat cctcgcccac ctggcgcgcg gcaacggtct
     1621 gtgggatggg cgcaccctga cactggacac cctgccgcaa tacgccatcc ccggcgcggt
     1681 cgccggcgcg gccacgccta ccctgccggc cgtcgcccaa acgacgcccg cgccctccac
     1741 gcccgcgccc acgtcggcgg ccctggccac cgggcagccg gcccacacgg gcggcatggg
     1801 cgcggccggc cggctggtac tcgtcggtgg cgtagtcgtc ctgtttgctc tgggaggttt
     1861 cgtgctatgg ctgagacgca gccgctccag ctagacctcg acgcgcccga tatgcatacc
     1921 ctgcccgcct accggcggct ggggctgctc aacgggctgc tcattggtct ggcgttgggt
     1981 ctgggggcgt ggggggtgga ggtcgtgcgg gcggcgcgtt tgccgctgcc gctgactgtg
     2041 ccggcgctgc tgctgggcat tctcctgctg accatcctgg gcgcggtcat cggctggctg
     2101 agcgcgcgca tcgcccgcac ctgggtgacg gtgctgctgt ggctggcggg cgcggtcgtg
     2161 gccagcctgg gcatcggcta cctgcccttc cacggccgca ccgtgaccgc ctggctggcc
     2221 gatccgcgct tcttcggccg caacgtctat cccaattcgc tgggcggctc caacctcggc
     2281 ctggtgctca gcggcctgtt catcctcgtc gtcctgggcg cgctggggtt gctgcaaagc
     2341 taccgcctgg agaacatggc cggcgagatg cacaaccggc gctggccgag ccggcgggcg
     2401 tggctggcgc tgctgctgcc gctgccgctg gttttcgtca gcgctctgtc cacgtccagc
     2461 ggcatggtca accccgcggc cacggccgcc gggctggcca accacgccat cctgcgggca
     2521 caggcctacg agggcgacct gcgcgacctg cccaacgagg ccggcatcag ctatctgggc
     2581 ctgcggccgg tgcatgacct catcgccggc ccattcactc tgggctttgt cgataacaac
     2641 gtggctacgg cgacggccac tgtggccgcg cacttcgaca gcggcaactg gatccattgc
     2701 agcatcatca acgaccagtt caatttctgc tacgatgcgc gactggtgta tgtcgatggg
     2761 ctgcgcggcc tgatcaccgg cgaagcgccg ccggagaatt gccacacctg cgacctgacc
     2821 gcgaccgacg aagccgcggc ctggctggcg gagcacagcg ccgccttcgg gccgcagccg
     2881 gtcgtgacgc tggaggcgca acagggcaac accgtcctgc tgcgggccgc cggcgaagat
     2941 ggtttcgccg cgctgtgctg ggtggaaggc atcgacccgc cgacggtgac cagctgcgcc
     3001 gaagagtgat ccgcgccaca gaacgaacac cgaggtgatt ggccgcaatg ccgccttttg
     3061 tcaaacaact cctcctgcgc ctgctggctt tgccgctgac gctgctgatc atcacagcgg
     3121 cgctctacgg cattatcatg ctcgccccgg ccgaggagcg ggcgcagctc tacctgccgc
     3181 cgcgcacgcc gccgctgacg caggaggaat cccagcggct catggcccgg ctggtgaccg
     3241 agcacggcct ggacgacccc tacccggtgc aatatgcccg ctggctgggc gatctgctgc
     3301 gcggcgattg gggctggagt cccacgttca aggccgacgt gctgacgctg ctgaaaaagc
     3361 gcctgccggt gacggctgaa ctggccttct acgccgtcct ggtcatcgtg cccacggcgc
     3421 tgatcggcgg ggtgctgtcc ggctggcgag ccaacggccg cttcgaccgc ggctaccggc
     3481 tggcgacgtt cgtggccacg tccatcccgc cgtttatcct ggggttgttc ctgctgtcga
     3541 tcttctacgt cggcctgggc tggttcccgc ccggccgcac gggcatctac gagttgtcgc
     3601 tcagcacgag cgacttccgc gctatcaccg gctttctgac cttcgacggc ctgctcaacg
     3661 gccggctcga tgtcgtcgcc gacgcgctgc ggcggctggt cttgcccgtc ttcagcctga
     3721 gtctgttgta ttgggccacc gtcggccgcg tcacgcgcgt ggccatgatc gaggagcaga
     3781 caagggacta cctgctgacg gcccgcgcca aagggctgcg cgagcgccgc ctggagtggc
     3841 gtcacgcctt ccccaacgcc gttggcccgg ccctgacggc catgatcctg tcggccgctg
     3901 gtctggtgac cggcatctac attgtcgagt cggtcttcgg cttcaaaggg ctgtcagagc
     3961 tggtgaccaa aggtttcttc gtggccccgg acgcgccgct ggtgatgggc ttcgccgtct
     4021 tcagcgtgct gctggtgctg cccatcattc tgctgttcga cctgctgcgc gtgaccctcg
     4081 acccacggct gcgcgaagac ggcctggagc tatagccgca tgcgccgccg attgcgcccc
     4141 ttccggcgtc gctcgacgct catcggcctg gccctggtgc tgggtttctg cctgctggcc
     4201 gcgctcgcgc ccgtgctggc cccgcccctc aacccggccg atccctggcc gttcatgaac
     4261 gtcagccaat cgttctcgcg ccagccgcag ccgccggggc cgggcttgcc gctgggcacg
     4321 acgccgcaga ttcagaacat gccccagtat ggcctcgctc cggggcagga tgcatcctac
     4381 cagtgggacg tgttctacac gctggtgtgg ggagcgcggt cggcgctgcg ctttggcctg
     4441 ctggtgacga tcctgacggc gctcatcggc atcgccgtcg gcgtcgtcag cggctacgcc
     4501 ggcgggctgt ccaacctgct ggccatgcgc gtcaccgacg ccttcctggc ctttccggtc
     4561 atcgccggcg tgtgggtcat ccagcgcgcc ttctatggca acatctacaa cccctttaac
     4621 gaccccacga ccctgaccgg ctgggaagcg tttctctatc gggcgcgcat cgacccgatc
     4681 atgatcgcct tcatcctctt ttcgtggatg ccctacgcgc gactgatgaa cgcgcaggcg
     4741 cggcgggtga agaacatgga ctacatccgc gccgccgagg cgatgggagc cacgggcggg
     4801 cgggtcatct ggcgccacct gctgcccaac accatcgccc cggccatcgt tctggccgcg
     4861 cgcgacgtgg gcggcatggt catcctggcc tcagcattca tcttcatcgg cttcggcggc
     4921 aacgtggcct gggggacgat gctcgtcagc agccgcgact tcgtcatcgg tctgaacggc
     4981 aacccgttcg cctactggtg gacgttcgtg cccatctcgc tggcgctggt cctgttcggc
     5041 gtaggctgga atttgctggg cgacggcctg aacgcggccc tcaaccccca cgggcgataa
     5101 tggctactcg taagggggcc aaaggcgaag aaccttaacc gtccgttcgt cgtccaggat
     5161 ttgatagacc agccggcgct gaatgttgat ccgccgcgag taagcgccgc tgagatcgcc
     5221 gacgagcttc tcaaaagggg gtggggtctg gtaggggttc tcgcgcagga cggccagcag
     5281 ggcctctgct cgcggccgca gcccggtggc ggtcagcctc ttggcgtctc gctgggcctg
     5341 tttggtgaag acgagacgcc acggccctgc tgtcaccact caatctcggt gtcgcattca
     5401 tctaccggcg tagccatccc gtcgatgatg gactcgcgca tgcccggaat gcccaacagg
     5461 tagagtgtct cctcaatcgc ccgccaatcc tcttcggcaa ccaggacggc attgccccgt
     5521 ttgccagtga tcaacaccgg ctcgtgggtt tcgctgacct ggttgagcag cttgtacagg
     5581 ttcgtacgcg cttcggtggc gctgagcgtc gtcatggaac acctccgcag gaaaggtgcg
     5641 tgatcgcgta cgttttgtca agcggccctc attcactcag gaatcgccgc gctcccagga
     5701 agcgctcgcg cagcgacgcc actgcctgca cgttgtccac gtagacgccg ttgcgcccgc
     5761 ggctgctgtg gatcatcccc caccccgccg gctccacctc cggccccatg ctgacgccga
     5821 cgtggctgac atcgcggtgg tcgcccggcg cgccgaagta gaacaagtcg cccggcgcaa
     5881 agggcggttc caccggccgc ccggcggcga actgctggtc ggcatcgcgc ggggtgacga
     5941 cgccggccag ctggtggagc aactgggcga agccggagca gtcgataccg tggacagacg
     6001 tgccgcccca gagatagggc acgccaatga attgcagcgc gtcgtgatga accatatggc
     6061 ggcggcggat gtcgagcggc cggtcgtcgg ccagcgggcg cagatccatc gggtcggcgt
     6121 agccctcccc ggccgcgctg gccagccgcg cccagccgtt gtcctgcgtc tccacagcta
     6181 cgggcgtgcc cgcgaagagg cgcgtgacca ccggggccag gtagttcggc ccggcgtaga
     6241 gcagcgtgaa gggcgcggca acctggtggg ttggcgatgg cgcggccgcc tcgctcaggt
     6301 aggcgcggta catccagccc agatagccgt cgtcgagccg gacgaagacc cagcgctcgt
     6361 cctccgccag cacctcgacc gccgcgccgg cccgcggctg gctctgctgt tcgcccagcc
     6421 acgacggcga gcgctgcaag ccggtcaggt tggtggccac ggtcaggcgc tgcggccgtg
     6481 cctggcgcag cacatccacc ccctcggcgt cccatcgcac gtcgggcaac tcttgccgca
     6541 agcgggcgag gacagcgtcg cgggtggccg tgtcgagcac gcggccttgc aggcagacgc
     6601 ggccgtcgcc gggggcaata gtcagttcgc aaacgtggaa gcgcccgtcg ccgaaggggg
     6661 cgcaggcttc ggccaacgcg gtcagtagtt gatcgtccat gtacatttgt ccttgtaggg
     6721 aaaaccctca cgcaaagacg caaagacgca aagaaaagat tcttcttggc gtctttgcgt
     6781 ctttgcgtga ggctcttcct tcttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnatcctt catcgtctta tgacgccgat gccgggccgg tcgggcacgt ggacgcggcc
     6961 ggcgtcgtcg taggtcaggc cgtcgaaggg gtcatcggcc accagccagg gcgcgtccag
     7021 gtctagccag tcagccaggc ccatcaggtg ggccatggcc gtggccccga cggccgtctc
     7081 gaccatgcag ccgagcatga cgcccagccc cagcgcgcgg gcgcggcgca acaccgccag
     7141 cccggcggcc agcccgccaa gcttcatcag cttcaggttg acgccgcgca cgccggccgc
     7201 ggccagccgg tcgacgtcgg ccagcgattg cagcgactcg tcggccacca cgggcacggc
     7261 gcaggccgcc tgcacccggc ccataccggc gatgtcgccc ttggccacag gctgctccac
     7321 cagggcgacg ccgtaaggca gcatccgctc gatctgggcg atggcgtcgt cgggcaacca
     7381 accagcattg gcatcgacgt aaagggtcac gtcgggccgg gcggcgcgca cggcggccac
     7441 gcgggccacg tcgccgccgt cgtcgccgcc cagcttgagc ttgatgacgg aacaatcatg
     7501 cagcgcggcg gcctgctcgg ccatcacctc cggcgcgtcg atgcccagcg tgaacgacgt
     7561 cggcagcggc cggggcacgg gtaggcccag cagggcgtgc agcggttggc ccaggcggcg
     7621 gccgatgcgg tcatgcaggg ccaggtcgag cgcggcgcga gccggggccg ggccggcggc
     7681 aatggccggt tcggcggcca gccaggcggg gatgccggcc gggtcgtcgg gaaagggggc
     7741 ggccaggcgg tcggccgcgg cccaaacggc ggccatagcc tcatcgctga tgccgtaata
     7801 gggcgggatg gtgccctcgc cccagccctc gtcgccggcc aggcgcaacc agtgggcgcg
     7861 gcgcgtctcg ctggccccgt agctgacgcg gaaaggcgtg cgcaggcgca gggtaatggg
     7921 ttcccaggta agtcgggtca tggtcgtatt atccgcgaat gtggccgcta ggctctcatc
     7981 agcagcgccg ccagcagggc gctgcgctcg accatcttgg agatgatgac gtgctcgtga
     8041 cgggcgtgcg cgccgtcgcc aaccgggccg aggccgtcga gagtggccgt gaaggggctg
     8101 gtcaggttgc cgtcggagcc gccgccaacg gccgtctccg tcagcgccag gcccatctcc
     8161 gcgcccacgt cccgcgccag ctcccacagg gcggcattgc gcggcgtgcg ctccagcggg
     8221 gcgcggtcga agccgccgct gaccgtcacc gtcgtgcccg gcagcggcgc gggcaggacg
     8281 cgaatggcct gctccagccg ttcggcgtcg accacggtca cggcgcggac gtcgaccacg
     8341 gcgcggcact ccggcgggat gatgttggtg cgcaacccgc cgctgatcag cccgacgctg
     8401 acggagacgc cgcgcgggta gtcgttgagc gtcgccagcg tgcggacgat ctcggccatg
     8461 cccacaatcg cattaatgcc cttctccggc tccagcccgg cgtgggcggc gcggccgtgg
     8521 acgacgatct cgaagtcgcc cgtgcctttg cgcgccgtct tcaacgcgcc gtccggcccg
     8581 gcggccggct ccagcaccag ggcccgcgcc gactcgcgcg ccaactgctc gatgaccggc
     8641 cgcgactcga tgctgccgat ctcttcgtcg gtgttgatca ggaaaacggg ggccagcggc
     8701 ggctccagcc ccagctcatg cagggcgcgt agggcgaata acccttgcgc cagcccagcc
     8761 ttcatgtcgt agctgcccgg cccgcgcagg acgtcgtcct gcacctccag cggcatctcc
     8821 gccagcgtgc ccacggccca gacagtgtcg cagtggccca ggaggagttg tgtcgggcgc
     8881 tccccggcgg ccgggtggcg ggcgatcagg tagccgccgg tgcgcgcgcc cggcgtgtgg
     8941 cggacgtggt agccgaggcg gtcgaattgc tcgtagagga tgccaatggg gccggcctgc
     9001 gtctccggcg cgtcggaggg cgactcggcg gcgacgaggt cggccagcag ggcgaccatg
     9061 tcggcgcgct ggccgcgaat gtggtcgagg atggtctgtg tggatgatac catagcgtaa
     9121 ttagaacctg tgcgcgccgg gtaggcaatg aagggattag gggctaggga ttaggttcca
     9181 ggttccaggg ggaagaacgc tcttccctgg aacctggccc ctggaaccta atcccttcga
     9241 tcattaactc ggcgttaaag cccagactct agacctcctg ccgctcgacc acgcccacgg
     9301 ccgtgatcag gcagaaggcg atcagcacca gcgacccggc cagcgcgccc cagccgttga
     9361 ccggctgtgg gtcggggata cccagctggc taatccaggc caccaggccg gccggcaaca
     9421 gcgccgcggc ctgcgggaag gccccgccga cgagcacgat gacgcagccc agcagcccca
     9481 gcccggcagc cagcagcgtc gtccggccga gggcgctgcc cagcagcacg acggcgacga
     9541 aggccagcag ccacagccac aggaagacgt tgccggccat gaacggcccc agcgccagcc
     9601 ccggctcgaa gagctgtccg gtgtagaacc acgcccccag cccggccagc aggagcgccg
     9661 ccagatagac gagcgcctgc gcgtcgaact tggccaggac gaacgcccag cgcgggatgg
     9721 gcttactgag cgtcatggcc gccgtgcccc gttcgcgctc cccagcgatc gcgcccatgc
     9781 ctagcaggat ggcgatgatg aaaccgaact gggtcaggtt tttgacgtac tgggtcacgg
     9841 cgtcggccgt cttcggttcc ggcaccaggc cagagaactg ctccgcgccg ggcacgctgc
     9901 gcaacaggtc ggccgtgaag cgggccagca gcggcgacgt tagcccgaag agaatgaaga
     9961 caacgccgac gaccagcgcc cggcgcgtgc gccactgctc cagcagttcc ttgcgcagca
    10021 gggcggcgta ttgcgcccac tcctcgcgca acggacgtgg gcgatggggc ggctcggcca
    10081 cgccatagcg ccggtaggtc cagccgatga gcgccagtcc gggcacgacc agcagggcca
    10141 ccagcccctc cagcagccag gcgttgctga ccgccggccc gatgtaaacg agcgcgccgt
    10201 ccacgagggc gtggtagagg acgccggcga tggcatagag gacgttgcgc cgccggaagg
    10261 ccagccagaa cagcagcgag atagccacgt ggagggccat cgccagcagg cgctcgacca
    10321 acggcagcag gaaggccgcc gagggcgtcg ccaccgcggc cagttgcgct tccaacgccg
    10381 ccagttgcgc cgccggcagg ttcagcgccg tcaggtctgt accccgcagg gcgaagaggc
    10441 cggccaccga cagagccgtg aacgcgccca ggatcatcgc ctccaggccg ccgtggccca
    10501 ggccaaccat gacgccgcct gaccaacgcc cgaccaaccc gcgccggtgg agcagccaga
    10561 aggccgcggc gcgcatcagc ccctcgcaca cgccggccga caggcccagg acgacggccg
    10621 tgcgcagcaa atccggcgcg ccggccgcca ccgggccgat caggccgatg tcggccagcc
    10681 agttgttaag tggcaggtgg taggcctgtg acagaaagaa ggccaccgcc ccggccgacc
    10741 agagcagcca cggcgcgcgg aagcggcggc gcagggccac agccacgccc acgggcacga
    10801 ggagcatcag ggctacggtg atcgcataaa ggatgctcat gccgcctcct cgctcaagct
    10861 gaggaaaatc tcctccagat tgggccgcac ccactcatag cgcaacagcg gcaagttgtg
    10921 ggcgacgaca agcggcagca gggcggcctg cccggcggcg gcgtcggccg cggccacgcg
    10981 gacgcggttg gcctcggtcg tgacgccggc cacccacggc tcggccgcca gcgcggcggc
    11041 gaaggcgggc agccgcccgg cggtttcctc gggcacgacc agttcggcca cgtcggaggt
    11101 gtagcgcgac agcaattcct cgcggccggc cacctcgatc agccgcccct cgtggaggat
    11161 gccgatcctg tcgcagacgc gctccacgtc gctgaggatg tgggtggaga agaagacggt
    11221 cacgtgggcg cggagggctt cgagcaggtc aaggacatcg cgccgcccgg cggggtcaag
    11281 gccggccatc ggctcatcga ggatgagtac gcccggccgg tggatgagcg cctgggccag
    11341 gccgaggcgc tgcttcatgc cgccggagaa accgcccacc cgccgccggg ccgcgcc
//
LOCUS       DAOSMQ010000029        11396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5672_length_11396_cov_4.824001, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000029 DAOSMQ010000000
VERSION     DAOSMQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5672_length_11396_cov_4.824001"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3090)
                     /locus_tag="PK829_01820"
     CDS             complement(<1..3090)
                     /locus_tag="PK829_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU39981.1"
                     /translation="MPSKLGPHFIGTPGLPRWLEAGARVFKFDPSSLGASDQIPAGPL
                     VVGKLDQREDHLDLTDWKAYMNGGGTPATVAAHRFNVQREIFVGGNKPRVDRYLVNPR
                     IDVWEDDNEVVPDNPDEARWYSDYCIEMMRLYESIGRRRANFCFAVGTPDVRPGDPAD
                     VWPHLLPAIRHAHDHGHYLALHEYMGYEADLGVGWKQIDSQRRLTRRWHGRRDPSGQP
                     DESYPYGYVALRYRLVYDTYLRPAGLAGVPLLITELGCDSVETVTPAGGSVGTWQEHR
                     AFWSAAGHDPEATYADMLTWYDHHIRQDAFVRGAMIFTVGSVGIWAKWDIAGTSVEER
                     VLTYITSEQNKEDETMTDRPQPVTPATQGAAAPAELASMNAAETAGFTSDTDMNRVPA
                     GAPFRATWTFRNSGQSTWSGDYRLAYADTPHPETVNNARSTLGAAASHTLAELGAPPV
                     RPGETVALTLTFHAPAAPGTYATNWQLAAPDGQRFGPVRWLRAVVVAVAEAGTLRYEV
                     VSFNNTAGDVNTLQPGQEFSGTWTLRNTGDRAWDGDFRVAYLDSAVNDTANTVRDPMG
                     AKPVATLRQASGRERVAPGETVAIRFDLVAPAAPGAYSFHWQLESATGEAFGGVRWLR
                     IGVVGAPDDTQRPRPTPDGGRVQFGMNVNPEVHGLDVERLSGLSWVRFVFFASRLNLS
                     PEEAYQRRYRAWIQTYAAAGIRSLVILHQDTEWGNAPWQNGGWEQYAETFAKACGRVA
                     AACSEFGDCVAYQIFNEQDSGPDNKSAIGIPAEHYALILDRAQEAIRRNHPGAVVIFG
                     GLNTGPEKAIAYTRAVQTRLGGRLPVDALAIHPYGRYVKKIIFNYGSIGKLSDSLDRF
                     HKAFPGKPIWITEVGAASDSVIGPEHYGDIADYMREFVDELIDNYADVVPVLIWFAWT
                     DRMRNSGILTADGKPKTGIFDVFQYMIARGKEIPEELESLAVSGLEALDVSKADFLSF
                     NTTLANHVAVPAGTAFTNRWRFRNSGATTWGDGFRLAYVPDGANSDPMTAQTDVGLSA
                     "
     gene            complement(3192..3968)
                     /locus_tag="PK829_01825"
     CDS             complement(3192..3968)
                     /locus_tag="PK829_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39982.1"
                     /translation="MAQNWHTLLVGGGRRDGDAETVLRLSLPAGGRGYADAQIDDYGG
                     PRPAGAPRFRHQPGLVLSLRARFSPDIAGTAGFGFWNAPYGDPATRRLALPAAAWFFY
                     ASAPNDLPFAPEPGRGWFAATLDSGAPRALALLPLAPATLLLNQFGPLRRRLWPAVRR
                     RLGISAAPLAAARPAATPDTWRDYRLEWRPDGCCFLVDGAPVLTTTTSPRGPLGFVCW
                     VDNQYLVLTPRGRFRSGYLPVASEQWLEVAEVAIRPPAKR"
     gene            4018..4665
                     /locus_tag="PK829_01830"
     CDS             4018..4665
                     /locus_tag="PK829_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU39983.1"
                     /translation="MEITWYGLSCFRITERKLATIVTDPFAPAVGLGELKLRADIVTI
                     SHDAPGHNYAGGITGAEHRLTGPGEYEIGSVFVTGIATPSKTGKFNNIVYLYDYNGLT
                     VAHLGDLGDVPSQKQIEDLEMVNILLVPVGGGSSLNAAGAAELVSMLEPNIVVPMHYQ
                     VPGLTMPLEPLEKFLKEMGVTEAKHETSLKIGGADSLPQETEVITLAFKEQVPAG"
     gene            4691..4993
                     /locus_tag="PK829_01835"
     CDS             4691..4993
                     /locus_tag="PK829_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39984.1"
                     /translation="MTAKLIMRWNVRHEMESEYFEFLVHELIPSMNKLGMGDIQVWYT
                     AYGSCEQKMAEGVMDSTDDMLRIMQTEEWGQLTEKLQGFVQDYSQKVITGTRGFQI"
     gene            complement(5003..5299)
                     /locus_tag="PK829_01840"
     CDS             complement(5003..5299)
                     /locus_tag="PK829_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU39985.1"
                     /translation="MPLYTYRCNENGHEFQARQRMVDDPLTECPVCGGPVRRVVSSVG
                     VVFKGSGFYVTDNRTSSVNGKGGKSKSKDAKEGGAAESSSSSATTPSAPSTPAE"
     gene            complement(5401..6711)
                     /locus_tag="PK829_01845"
     CDS             complement(5401..6711)
                     /locus_tag="PK829_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU39986.1"
                     /translation="MTGRVPPRGGREWAGWFGRAPWRILAVGMLLALGVATALFSALM
                     APGPADLAQLVATLAVAAVLAVALGYLFYRRGWARSSSLMVTMVMTYLWAALVTLLPV
                     WLLQRQMFFSEHDLALSGVLLLFAAIVATTYGLFIATSVADGLRQMAAAANRLAAGDP
                     AARAPITGRDEVARLGASFNAMADQLQAAAHRREEVETLRRNLIAWTSHDLRTPLTAI
                     RVRVEALHDGIVTEPEEVRRYVAAMRADVVALDSLLDDLFELAQLDAGGPPAARTLHA
                     PADFVADALERFRPLAERRDIDLIGEATTTAAVAVEPGKIERVLGNLIGNALRHTPPG
                     GQVTLAAGETPEGVVFRVEDTGPGFSAADLAYGFEQFYRGEEARTRATGGAGLGLAIA
                     RGIVQAHGGHIWAENRAEGGARVGFLLPRAGKVAAGSGVPLTGN"
     gene            complement(6708..7424)
                     /locus_tag="PK829_01850"
     CDS             complement(6708..7424)
                     /locus_tag="PK829_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU39987.1"
                     /translation="MTTGPGLEGDILIVDDEATVVEVVSLYLRREGFRVRAARDGAEA
                     LLAIGAERPALIILDIMLPHIDGLSLMRRLRQNPAANVPVILLTARGQEADRIAGLDL
                     GADDYVTKPFSPGELVSRVKAVLRRATRPADGGDAPIDHGELRLDPLTREVVVRGRPV
                     ELTATEFNLLWFLAAHPRQVFRRDKLLEEVWGYTDYLDPGTVTVHIRRLREKIERDPA
                     KPAWLQTVWGVGYKFEPGAE"
     gene            complement(7421..8602)
                     /gene="queA"
                     /locus_tag="PK829_01855"
     CDS             complement(7421..8602)
                     /gene="queA"
                     /locus_tag="PK829_01855"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOU39988.1"
                     /translation="MLSSAGHGATEHGASGHRVADYDYALPEALIAQQPIEPRDAGRL
                     LVLDRDSGAIAHRRFRDLPDYLRPGDILVANDTRVRPARLFARKPTGGRVELLLLEQR
                     GDAWAALVGGRGVAEGLTLTLLDHAGQPAEITAAVAGRGPEAQRLLRFSAPVETWIEA
                     LGYTPLPPYIHTALDDPERYQTVYARPAGSAAAPTAGLHFTPETLLDLRARGVLFATV
                     TLHVGLDTFKPVSVASVDDHVIHSEWARLSPETAQRINEAHLAGGRIVAVGTTTARVL
                     ETAALRSAGITGSLQTISARDAAGETSALCPWRPVAAFEGPTDLYIYPGYRFRAVEAL
                     LTNFHLPRSSLLMMVSAFAGREAVLAAYRAAVAEGYRFYSFGDAMFIGSHRAGAPEPA
                     P"
     gene            complement(8592..9581)
                     /locus_tag="PK829_01860"
     CDS             complement(8592..9581)
                     /locus_tag="PK829_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU39989.1"
                     /translation="MNNKYIAIEGVIGVGKTTLARLLQPRYSASILLEVVEDNPFLPK
                     FYSDRERYAFQTQIFFLLSRYHQQYQAIPAALQKGALISDYTFAKDELFAWLNLKDDE
                     LAMYGRVHAALGEKILRPDLIVFLRADHDVLMRRIALRDRPFERDMDPDYIRELAEAY
                     DAWLSRLTDIPVLTIETNHLDYLSRAEDLDRVTAMIAAELERAAPAGAPEAQLRSLQQ
                     GRVAAFQQFHREREATNGTAVDPLSHYLLLVEEVGELATALRHVAADSRAAAGNPAAL
                     AEAIATHRPALREELADLLAQLLKLANDTGIDLEQAYLDKMRLQLAAAVPHAL"
     gene            complement(9587..10354)
                     /locus_tag="PK829_01865"
     CDS             complement(9587..10354)
                     /locus_tag="PK829_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOU39990.1"
                     /translation="MHCLIIAGGQVRPEDPLYPYTNGRPKALIDIGGQTMLERVVGAL
                     QASRHVEDILIVGLDPVAAGQRFARPVESLPDQGSMVGNMLAGCDWFRHHRPDAPVIL
                     GCSADIPAITPAMVDAFIEACRPWDHAVYYNFVTRETLESRFPNSNRTYSRLNGLEVA
                     GGDMVMAQPTVADRNRALIDMLTQARKHPWRIARIVGLPFLLKFLFHRVTFADIEATA
                     GRLIGAPVKIVLGSPAEMAMDADKPYQVDMLRAVIAP"
     gene            complement(10560..>11396)
                     /locus_tag="PK829_01870"
     CDS             complement(10560..>11396)
                     /locus_tag="PK829_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU39991.1"
                     /translation="DDQGHGTHTMGTMVGTQPDPAVGIGMAPGAQWIGCRNMEDGWGA
                     PASYAECYEWFIAPYPFGGDPFSDGDPSRAPDVINNSWSCPPVEGCAAGDVLLAAVEA
                     VRAAGILSVHSAGNNGSGCETIDTPAAIYDASFTVGATDHDNVIADFSSRGPVMIDES
                     GRLKPDVVAPGVDVRSSYLNGGYATLQGTSMAAPHVAGLAALLISADPALAGQVDALE
                     ALITANSVHLTATQDCGNITNRVPNNVYGWGRIDALAAYEALEPSQTWPFHTFVPAAP
                     GQ"
BASE COUNT         1818 a         3679 c         4066 g         1833 t
ORIGIN      
        1 cgcggacagg ccgacgtcgg tctgagccgt catcgggtcg ctgttggccc cgtcgggcac
       61 gtaggccagc cggaagccgt cgccccaggt cgtcgcgccg ctgttgcgga agcgccaacg
      121 gttggtgaag gccgtcccgg cgggcacagc gacgtggttg gccagggtgg tgttgaagga
      181 caggaagtct gcctttgaca catccagcgc ctctagtcca gagacggcca acgactccag
      241 ttcctccgga atctccttgc cgcgggcgat catatactgg aagacgtcga agatgccggt
      301 cttgggcttg ccgtcggccg tcaggatgcc agagttgcgc atccggtctg tccaggcgaa
      361 ccagatcagc accggcacga cgtcggcgta gttatcgatc agttcatcga cgaactcgcg
      421 catatagtcg gcgatgtcgc cgtagtgctc cgggccgatg acgctgtcgc tggccgcgcc
      481 gacctcggtg atccagatag gcttgccggg gaaggccttg tggaagcggt cgagtgagtc
      541 gctcagcttg ccgatggagc catagttgaa gatgatcttt ttgacgtagc ggccgtaggg
      601 gtggatggcc agcgcgtcga ccggcagccg gccgcccagc cgggtttgca ccgcgcgcgt
      661 gtaggcgatg gccttctccg ggccggtgtt caaaccgccg aagatgacga ccgcgccggg
      721 gtggttgcga cggatggcct cttgcgcccg gtcgaggatg agggcgtagt gctcggcggg
      781 gatgccgatg gccgacttgt tgtcggggcc gctgtcctgc tcgttgaaga tctggtaggc
      841 gacgcagtcg ccgaactcgg agcaggccgc ggccacccga ccgcaggcct tggcgaacgt
      901 ctcggcatac tgctcccagc cgccattctg ccacggcgcg ttaccccact cagtatcctg
      961 gtgcaggatg accagcgagc ggatgccggc ggcggcgtag gtctgaatcc aggcccggta
     1021 gcgacgctgg taggcctcct ccgggctaag gttgaggcgc gaggcgaaga agacgaaccg
     1081 cacccagcta agcccgctca gccgctccac gtccaggccg tgcacttccg ggttgacgtt
     1141 catgccgaac tgcacccggc cgccgtctgg cgtcggccgc ggtcgctgcg tgtcgtcggg
     1201 cgcgcccacc acgccaatgc gcagccagcg cacgccaccg aaggcctcgc ccgtagcgct
     1261 ctccaactgc cagtggaagc tgtaggcccc cggcgcggcc ggagccacca ggtcgaagcg
     1321 gatagccacc gtttcgccgg gggcgacgcg ctcccggccg ctggcctggc gcagcgtggc
     1381 gaccggcttg gcccccatcg ggtcgcggac ggtgttggcc gtgtcgttca cggcgctgtc
     1441 gaggtaggcc acgcggaagt cgccgtccca ggcgcggtcg ccggtgttgc gcagcgtcca
     1501 ggtgccgctg aactcctgcc ccggctggag ggtgttgacg tcgccggccg tgttgttgaa
     1561 gctgacgacc tcatagcgta gcgtgcccgc ctcggccacg gcgacgacga cggcccgcag
     1621 ccagcggacg gggccgaagc gctggccgtc gggcgcggcc aactgccagt tggtagcgta
     1681 ggttcccggc gcggccggcg cgtggaaggt cagcgtcagg gcgaccgtct cgcccggcct
     1741 caccggtggc gcgcctaact cggccagggt gtggctggct gccgcgccca gcgtcgagcg
     1801 ggcgttgttg actgtctcgg ggtggggcgt gtcggcgtag gccagccggt agtcgccgct
     1861 ccaggtgctc tggccgctgt tacggaacgt ccacgtggcc cgaaagggcg cgccggccgg
     1921 gacgcggttc atgtccgtgt cggacgtaaa gccggccgtt tcggcggcat tcatgctggc
     1981 caactcggcc ggggcggcgg ctccctgggt cgcaggcgtg acgggctgcg ggcgatcggt
     2041 catggtctca tcttccttgt tctgttcgga cgtgatgtag gtcaggacgc gctcttcgac
     2101 gctcgtcccg gcgatgtccc acttggccca gatgccgacg gagccaacgg tgaagatcat
     2161 cgcgccgcgc acgaaggcgt cctggcggat gtggtggtca taccaggtca gcatgtcggc
     2221 gtaggtcgcc tccgggtcgt ggccggcggc gctccagaag gcgcggtgct cctgccacgt
     2281 gccgacgctg ccgccggcgg gcgttactgt ctcgacgctg tcgcagccca gttcggtgat
     2341 cagcagcggc acgccggcca ggccggccgg gcgcagatag gtgtcgtaga cgagccggta
     2401 gcgcagggcg acgtagccat aggggtagct ctcgtccggt tggccgctgg ggtcgcggcg
     2461 gccgtgccag cggcgcgtca gccggcgctg gctgtcgatc tgcttccagc ccacgcccag
     2521 gtcggcttcg tagcccatgt attcgtgcag ggccaggtaa tggccgtgat cgtgggcgtg
     2581 gcggatggcc ggcagcaggt ggggccagac gtcggccggg tcgccggggc ggacgtcggg
     2641 cgtgcccacg gcgaagcaga agttggcccg gcggcggcca atggactcgt acaggcgcat
     2701 catttcgatg cagtagtcac tgtaccagcg cgcctcgtcg gggttgtcgg gcacgacctc
     2761 gttgtcgtcc tcccacacgt cgatgcgcgg gttgaccagg tagcgatcga cacgcggctt
     2821 gttgccgccg acgaagatct cccgctggac gttaaaacgg tgggcggcga cggtcgccgg
     2881 cgtcccgccg ccgttcatgt acgccttcca gtcggtcagg tcgaggtggt cttcacgctg
     2941 gtcgagcttg ccgacgacca gggggccggc cggtatctgg tcactcgcgc cgaggctgga
     3001 cggatcgaac ttgaagacgc gcgcgccggc ttcgagccag cgcggcagtc caggcgtacc
     3061 aatgaaatgg ggcccaagtt tgctcggcat gacctcactt cctgttactg cgagatcggt
     3121 gtcatggtcg ccgcgacaca gagagactgt ctggaaattc taaaggaaaa gccgggtacg
     3181 tgtcaattaa atcaacgttt ggccggcggc cggatggcga cctcggccac ctctaaccac
     3241 tgttcgctgg ctacgggcaa gtacccgctc cggaagcggc cgcgcggcgt caggacaagg
     3301 tactggttat cgacccagca cacgaagcca agcggcccgc gtgggctagt cgtcgtcgtc
     3361 aggacgggcg cgccatcgac gaggaagcaa cagccgtcgg gccgccattc gagccggtaa
     3421 tcgcgccagg tgtcgggcgt ggccgccggt cgcgccgccg ccagcggcgc ggcgctgatg
     3481 cccagcctgc ggcggacagc tggccatagc cggcggcgca acgggccgaa ctggttcagc
     3541 agtagcgtcg ccggggcgag cggcagcaat gccagggcgc gcggcgcgcc gctatcgagc
     3601 gtggccgcga accagccgcg gcctggttcc ggcgcgaagg gcaggtcgtt gggggcagag
     3661 gcgtagaaga accacgccgc ggcgggcagg gccagccgtc gcgtagccgg gtcgccgtag
     3721 ggcgcgttcc agaagccgaa gccggccgtg cccgcgatgt cgggcgaaaa gcgcgcccgt
     3781 aacgatagga ccagcccggg ctgatggcga aagcggggcg cgcccgccgg ccgcgggcca
     3841 ccatagtcgt cgatctgcgc gtcagcatag ccacgtccgc ccgcgggcaa gctcagacgc
     3901 aggacggtct cggcgtcgcc atcgcggcgg ccgccaccaa cgagcaaggt gtgccagttt
     3961 tgagccatga gttccctcgg ctataatagc cctgatcttt tgaggagcat caccctaatg
     4021 gaaatcacct ggtatggtct gagctgtttc cggattaccg agcgcaagtt ggctaccatc
     4081 gtcactgacc cgttcgcgcc ggccgtgggg ctgggcgaac tgaagctgcg cgccgatatc
     4141 gtcaccatca gccacgacgc cccagggcat aactacgccg gcggcatcac tggcgcggag
     4201 catcgcctga ccggccccgg cgaatatgag atcggcagcg tcttcgtcac gggcatcgcc
     4261 acgcccagca agacaggcaa gttcaacaac atcgtctatc tatatgacta caatggcctg
     4321 actgtcgccc acctgggcga cctcggcgat gtgccctcgc agaagcagat cgaagacctg
     4381 gaaatggtca acatcctgct tgtacccgtg ggtgggggca gcagtcttaa cgccgccggc
     4441 gcggccgaat tggtctctat gctagagccg aacattgtcg tgcccatgca ctaccaggta
     4501 cctggcctga cgatgcctct ggagccgctg gaaaagttcc tgaaggaaat gggtgtcacc
     4561 gaagccaagc acgagaccag cctgaagatt ggtggggccg atagcctgcc ccaggagacc
     4621 gaagtcatca ccctggcctt caaagagcaa gtcccggcgg ggtagcccta gcaccagcgg
     4681 ggaaaacatc atgaccgcaa agctgatcat gcgctggaat gtgcgtcacg agatggaatc
     4741 ggagtacttc gagttccttg tccacgaact gatcccgtcc atgaacaagt tgggcatggg
     4801 cgacattcag gtgtggtaca cggcctatgg ctcgtgtgag cagaagatgg ccgaaggggt
     4861 catggactcc accgacgata tgctgcgcat catgcagacc gaagagtggg ggcagttaac
     4921 agagaagtta caggggtttg tgcaggacta tagccagaag gtcattaccg gcacgcgcgg
     4981 ctttcaaatc taagaccggc acttactcgg ctggtgtcga gggggcgctc ggcgtcgtgg
     5041 cgctactgga cgacgactcg gctgcgccgc cctctttggc gtccttgctc ttggacttgc
     5101 cgcccttgcc gttgacgctg ctggtgcggt tgtcggtcac gtagaagccg ctgcccttga
     5161 agaccacgcc cacggaactg accacgcggc gcaccgggcc gccgcacacc ggacactcgg
     5221 tcagcgggtc gtccaccatg cgctgccgcg cctggaactc gtggccgttc tcgttgcatc
     5281 gatacgtata caatggcatc tgtcgtaacc tcggtcgcgc tctcgcgctc cttgtcagcc
     5341 ggtgattcta gctgctgtcg cccgtcctga caaaccacat tgtattatag ccgccgcgcg
     5401 ttagtttccg gttagcggca cgccggagcc ggccgccact ttgcccgccc gcggcagcag
     5461 gaagccaacg cgcgcgccgc cctcggcccg attctcggcc caaatatggc cgccatgcgc
     5521 ctggacaatg ccgcgggcga tagccaggcc cagccccgcg ccgcccgttg cccgcgtgcg
     5581 cgcttcctcg ccgcggtaga actgctcgaa gccataggcc agatcggcgg cgctgaagcc
     5641 ggggccagta tcctcgacgc ggaaaacgac accctctggc gtctcccctg ccgccagcgt
     5701 cacctgcccg cccggcgggg tgtggcgcag cgcgttgccg atgagattgc ccagcacgcg
     5761 ctcgatcttg cccggttcga cagcgacggc cgcggtcgtc gtcgcttcgc cgatcaggtc
     5821 gatatcgcgt cgctcggcca gcggccggaa gcgctccagc gcgtcggcca cgaagtcggc
     5881 cggcgcgtgg agcgtgcgcg cggccggcgg cccgccggcg tccagttggg ccaactcgaa
     5941 taggtcgtcc agcaggctat ccagggcgac gacgtcggcg cgcatggcgg cgacgtagcg
     6001 ccgcacctcc tcaggctcgg tcacgatgcc gtcgtggagc gcctcaacgc ggacgcggat
     6061 ggctgtcagg ggcgtgcgca ggtcgtggga ggtccaggcg atgagattgc ggcgcagcgt
     6121 ctcgacctct tcgcgccggt gggcggcggc ttggagctgg tcggccatgg cgttgaacga
     6181 cgcgcccagg cgggcgacct cgtcgcggcc ggtgatgggc gcgcgcgccg ccgggtcgcc
     6241 cgcggccagc cggttggccg cggcggccat ctgccgcagc ccgtcggcga cgctggtggc
     6301 gatgaacagg ccgtaggtgg tggcgacgat ggcggcgaag agtagcagca cgccgctgag
     6361 ggccaggtcg tgctcgctga aaaacatttg ccgctgcaac aaccagaccg gtagcagtgt
     6421 caccagggcc gcccacaggt aggtcatcac cattgtgacc atcagcgacg acgaccgcgc
     6481 ccagccgcgc cggtagaaca gatagcccag ggcgacggcc agaacggcgg ccacggccaa
     6541 tgtggccacc agttgggcca ggtcggccgg ccccggagcc atcagcgcgc tgaacagcgc
     6601 cgtggccacg cccaacgcca gcagcatgcc cacggccaga atgcgccacg gcgcgcggcc
     6661 aaaccagccc gcccattccc ggccgccgcg cggcggcacg cggccggtca ttcggccccc
     6721 ggctcgaact tgtagccgac gccccagacc gtttgcagcc aggccggctt ggccgggtcg
     6781 cgctcgatct tctcccgcag gcggcggatg tggacggtga ctgtgcccgg atcgaggtag
     6841 tcggtgtagc cccagacttc ctccagcagc ttgtcgcggc ggaagacctg ccgcggatgg
     6901 gcagccagga accacagcag gttgaactcc gtggccgtca gttccaccgg gcgaccgcgc
     6961 acaacgacct cgcgcgtcag cgggtcaagg cgcaattcgc cgtggtcgat gggcgcgtca
     7021 ccgccatcgg ccgggcgggt ggcgcggcgc aggacggcct tgacccgcga caccagttcg
     7081 ccaggcgaga agggcttggt cacgtagtcg tcggccccca ggtcaaggcc ggcgatgcgg
     7141 tcggcttcct gtccgcgcgc cgtcagcagg atgacgggca cattggccgc ggggttctgc
     7201 cgcaggcggc gcatcaggct cagcccgtcg atgtgaggca gcatgatgtc gaggatgatc
     7261 agcgccggcc gctcggcccc gatggccaga agcgcctcgg ccccgtcgcg ggcggcgcgc
     7321 acacggaagc cctcgcgccg caggtagagc gatacgacct cgacgaccgt ggcctcgtcg
     7381 tcgacgatga ggatgtcgcc ttcgagaccg gggccggtgg tcatggggcc ggctccggcg
     7441 cgccggcgcg gtggctgcca atgaacatgg cgtcgccaaa tgaataaaag cggtagcctt
     7501 cggccacagc ggcgcggtag gcggccagaa cagcctcgcg cccggcgaag gctgagacca
     7561 tcatcagcaa gctggagcgc ggcaggtgga agttggtcaa caaagcctcc acggcgcgga
     7621 agcgatagcc ggggtagatg tagaggtcgg tcggcccctc gaaggcggcg accggccgcc
     7681 aggggcagag cgcgctggtc tccccggcgg cgtcgcgggc gctgatggtc tgcaagctgc
     7741 cggtgatgcc ggccgagcgc agggcggctg tctccagcac gcgcgccgtc gtcgtgccca
     7801 cggcaacgat acgcccgccg gccagatggg cctcgttgat gcgctgggcc gtctccggcg
     7861 acaggcgcgc ccactcgctg tggatgacgt ggtcgtcgac ggacgcgacg ctgaccggct
     7921 tgaaggtatc cagcccgacg tggagcgtca ccgtggcgaa gaggacgccg cgcgcgcgca
     7981 agtccaggag cgtctccggc gtgaagtgca gccccgccgt gggcgcggcg gccgagcccg
     8041 ccgggcgggc gtagacggtc tgatagcgct ccgggtcgtc gagcgcagtg tggatgtagg
     8101 gcggcagggg cgtgtagccg agggcctcga tccacgtctc gaccggggcg ctgaagcgca
     8161 gcagacgttg ggcctctggg ccgcggccgg cgaccgcggc ggtgatttcc gccggctgcc
     8221 cggcgtggtc gagcagcgtc agcgtcagtc cctcggccac gccccggccg ccaacgaggg
     8281 ccgcccaggc gtcgccgcgc tgctccagca gcagcagctc aacccgaccg ccggtcggct
     8341 tgcgggcgaa aaggcgggcc ggccgcacgc gcgtgtcgtt ggccaccagg atgtcgccgg
     8401 ggcgcaagta gtcgggcaga tcgcggaagc ggcgatgggc aatcgcgccg ctgtcgcggt
     8461 cgagcaccag cagccggccg gcgtcgcgcg gctcgatggg ctgctgggcg atgagcgcct
     8521 ccggcagggc gtagtcgtag tcggcgacac gatggccgct ggcaccgtgc tcggtggcac
     8581 cgtggccggc gctagagagc atggggcaca gccgcggcca gttgcagacg cattttgtcc
     8641 aggtaggcct gttccaggtc gatgccggtg tcgttggcta acttcagcag ttgggccagc
     8701 agatcggcca actcctcgcg cagcgccggg cggtgggtgg cgatggcctc ggccagggcc
     8761 gccgggttgc cagccgcggc gcggctgtcg gccgccacat ggcgcagcgc cgtggccagc
     8821 tcgccgacct cctcaaccag cagcaggtag tggctcagcg gatcgacggc cgtgccgttg
     8881 gtggcttcgc gctcgcggtg gaactgttgg aaggcggcca cgcgcccctg ctgaagcgac
     8941 cgcaactggg cctccggcgc gcccgccggc gcggctcgct ccagttccgc ggcgatcatc
     9001 gccgtcaccc ggtcgagatc ctccgcccgc gacaggtagt ccagatggtt ggtttcgatg
     9061 gtcagcacag gaatgtcggt caggcgcgac agccaggcgt cgtaggcctc cgccagctcg
     9121 cggatgtagt cggggtccat gtcgcgctcg aagggccggt cgcgcagagc gatgcggcgc
     9181 atcagcacgt cgtggtcggc gcgcaggaag acgatcaggt cgggccggag aatcttctcg
     9241 cccagcgcgg cgtggacgcg gccgtacatg gccagctcgt catccttcag gttcagccag
     9301 gcgaacagtt cgtctttggc gaaggtatag tcggagatga gcgcgccctt ttgcagcgcc
     9361 gcggggatgg cctggtactg ctggtggtag cggctcagca gaaagaaaat ctgcgtctgg
     9421 aaggcgtagc gctcccggtc gctgtagaac ttgggcagga aggggttgtc ctcaacgacc
     9481 tctagcagga tgctggcgct gtagcgcggt tgcaacagac gggccagcgt tgtcttgccg
     9541 acgccgatga cgccttcgat ggcgatgtac ttgttgttca tagtccttag ggcgcgatga
     9601 cggcgcgcaa catatcgacc tggtagggct tgtcggcgtc catggccatc tcggccgggc
     9661 tgcccagcac gatcttgacc ggcgcgccga tgaggcgtcc ggcggtggcc tcgatgtcgg
     9721 caaaggtcac ccggtggaaa aggaacttca gcaggaaggg cagcccgacg atgcgggcga
     9781 tgcgccaggg gtgtttgcgc gcctgggtca gcatgtcgat cagggcacgg ttgcggtcgg
     9841 ccacagtggg ctgggccatc accatgtcgc cgccggcgac ctccaggccg ttgagcctgg
     9901 agtaggtgcg gttggagttg gggaaacggc tttctaatgt ctcccgggtg acgaagttgt
     9961 aatagacggc gtggtcccac ggccggcaag cctcgatgaa ggcatcgacc atcgccgggg
    10021 tgatggccgg gatgtcggcc gagcagccga ggataaccgg cgcgtcgggc cggtggtgcc
    10081 ggaaccagtc gcagccggcc agcatgttgc cgaccatgct gccctggtcg ggcagcgact
    10141 cgaccggccg ggcaaaacgc tgtccggccg cgacggggtc aaggccgacg atgaggatgt
    10201 cctccacgtg gcgcgaggct tgtagcgcgc cgacaacgcg ctccagcatc gtctggccac
    10261 ctatgtcgat gagcgccttg ggccggccat tggtataggg atagagcggg tcttcggggc
    10321 ggacttgccc tccggcaatg atcagacagt gcatgacgtc atgacctctg gcaatatggc
    10381 gcaattgtaa cggctttgcg acggccgtaa aggcatgggc gccattgcga cagggcattg
    10441 ccaagtttgg aagcaggttc acgcgaaggc gccaagtaag ccaaggacgc gaagaagaag
    10501 ctttgttatc tgctgtttct tctttgcgcc ttggccactt ggcgacttgg cgtgagctcc
    10561 tattggccgg gcgcggcagg cacaaaggtg tggaacggcc atgtctgcga tggctccagc
    10621 gcctcatagg cggccagcgc gtcgatgcgc ccccagccgt agacgttgtt ggggacgcga
    10681 ttggtgatgt tgccgcaatc ctgcgtcgcg gtcaggtgga cgctgttggc ggtaatgagc
    10741 gcctccagcg cgtccacctg cccggccagc gccgggtcgg ccgagatgag cagcgccgcc
    10801 aagccggcca cgtgcggcgc ggccatgctg gtgccttgca gggtcgcgta gccgccattc
    10861 aagtagctgg agcgtacatc aacgcccggg gcgacgacat cgggcttcag acggccgctc
    10921 tcgtcgatca tgactggccc acggctgctg aagtcggcga tcacgttgtc gtggtccgtc
    10981 gctccaacgg tgaacgaggc atcgtagatg gccgccggcg tgtcgattgt ctcgcagccg
    11041 gaaccgttgt tgccggccga atgaacggac aggatgccgg cggcgcgaac ggcctcgacc
    11101 gcggccagta gtacgtcgcc agcggcgcag ccctcgacgg gcgggcacga ccacgaattg
    11161 ttgatcacgt ccggcgctcg cgatggatcg ccgtcggaga agggatcgcc gccgaagggg
    11221 tagggggcga tgaaccactc atagcactcg gcataggagg ccggcgcgcc ccagccatct
    11281 tccatgttgc ggcagccgat ccactgcgcg ccgggggcca tgccgatgcc gaccgccggg
    11341 tctggctgcg tgccgaccat ggtgcccatc gtgtgcgtgc cgtggccctg gtcgtc
//
LOCUS       DAOSMQ010000030        11382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5683_length_11382_cov_4.098702, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000030 DAOSMQ010000000
VERSION     DAOSMQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5683_length_11382_cov_4.098702"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PK829_01875"
     CDS             complement(<1..843)
                     /locus_tag="PK829_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39992.1"
                     /translation="MIPTFRRSSLRRVFSISAAVLVLAIVLVAPALAAPTPTVTLDLP
                     DSPFLGEPFTFTATFDNTGAGAQAGYGPYIDLFLPIGGADGTSAGGPNDGASFISATY
                     LGTAVTSATLTCAPGATINHPYVPGMTVVCPALPVGFDPSFTWQFVSLQLPFGSFTPG
                     QPPAEVTITADMSDFADLDKALPFQSRGVFRYGNDPLDNPGSDPAIVTPFTQETLTPT
                     LMTLDKVYIGPEDETATGPNFPRRYELNVNIADAQTIDELTVTDELPGNLQYIGIFST
                     TPAAT"
     gene            complement(1189..2649)
                     /locus_tag="PK829_01880"
     CDS             complement(1189..2649)
                     /locus_tag="PK829_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU39993.1"
                     /translation="MAQDRNTLLDFLSRHYTLDGLKTLCFNLFVDYDNLSGDTKNGKG
                     RELILHLERAGRLGDLEAALGRQQPRAYEREFLRPPPVPPIPARPNRDPRQVFISHAH
                     EDDGFAHRLAADLCAHGWNVWIAPDSIAPGETWVDAINRGLEASSYYVLALTPAASAS
                     PWVNTETNVAISLEHARLMRFIPLDVAPSRPPPLWTAYQNVPFRDDYQTGLEHLLARL
                     EGRPPRPQPAGDPAKRRVDEMRLFSDRQLHERARIELVRVAAGAFLYGAREADPRHER
                     PQRSVRLGEYWISRAAVTNAQFARFVEATGYTTTAEASGFGRVWVGGRWQDVERAYWR
                     RPEGPRSSIEERMHHPVVSVSWHDAQAFCEWAGLRLPSEAEWEKAARGSDGRLFPWGD
                     EPPSGGRANFGMGQGATTAVGCFSPAGDSPYGALDMAGNVWEWTTAEGSGGSARVVRG
                     GAWPSEARHLRVTWRVEVEPAQSFNTVGFRVIAPAR"
     gene            2882..3673
                     /locus_tag="PK829_01885"
     CDS             2882..3673
                     /locus_tag="PK829_01885"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HOU39994.1"
                     /translation="MSNRSILRCLFIVAVLAVSVAAAQAEPNAPAAVRVRVAHLAPFD
                     GSDAASIAVHVDGAAVGGTMAYGGHSAYITLPGAAGDHLIEVLRDGAVVLAEPHALAD
                     GDTSIVVIGDDSQVPLDLLVLDENLAEPGVGRAGLRITHVAAIGATIDDTKVDICSQT
                     GELFNATAAGLRYTRTTSYRLLPVGDYDLKITRLNAGGSCLGPVVIDPPVLTLAEGTK
                     TTLFLVGDGTNQTLAVFTFADGLIGDDNPPATGGTLFLPVLAASS"
     gene            3774..5393
                     /locus_tag="PK829_01890"
     CDS             3774..5393
                     /locus_tag="PK829_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="jacalin-like lectin"
                     /protein_id="HOU39995.1"
                     /translation="MEPKQLGPIGGEGGRPFDGYTIPDDARLTAVHVFAEWVLDALRL
                     EWVDSNGAPGSHPPIGGLGGYHHTFYLDEDEYLTGISGRCGWYVDSVRFHTNKRVSDL
                     FGGEGGEREYTLLASPGEEVAGLFGRCDWYIDALGLQLRPLAVREEAAEGAALEAEAE
                     AERLAEAAEEELDEALVDAAAMAMEGEDVLRASIAHRSEALTWLRSDPEAMEAEATPA
                     PAEADALADLVAALEEEIDAEQQERAAEEALDMAAGRVADFPPVEASLLEWTGEEGEP
                     LDAVVAVRRADVASELEVEALEEAAIAEAIAVLHEGHGGEGTVDLAVYTQVDESDEGD
                     VATVLAVAAPASGEEPPAGESETAIMVVDAIASEDELLELEQAAVEGALLALEEDLGE
                     AIDDADVAIYMGMTEDETGQPYGAVVVVASRSPVAPAATMRSATERGSFSDKVMQFGG
                     LEPRPQDLQLVEGIGPKIAALLIENGVNDLGQLAETPVERLREVLAGAGRRFRLADPA
                     TWPRQAHLAATGQWAELTQLQTELKAGREER"
     gene            complement(5441..6679)
                     /locus_tag="PK829_01895"
     CDS             complement(5441..6679)
                     /locus_tag="PK829_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU39996.1"
                     /translation="METQSKNRFVRGLMIMGVLLLALLLASAVAAQDAPSAQDAPAAA
                     TAATLTLGVTTTPAGGQGFWLTAVSFQGSWGRFGKGNGQYRQPRDVDQDAAGNIYVSD
                     HRNSRIQKLSATGVFIEKIGRSGRGAGRLLRPNAIAISGNLLFVANTENHRITVYNTT
                     SGALVREWGGQGTTNGKFNMPNGVAIGPDNNVYVSDTFNHRIQVFTQEGTFLRQWGSQ
                     GAGDGQLNFPAHLDFDGAGNLYVADSNNNRIVVYNTDGVFQRKFGVSGTGPGQFRVPV
                     GVEVGADGYLYVSEAFGNRIQKLTTNGVYIAHWSEAGGAAISRPNGLLVVGNRVYATD
                     LDANRIQIYAQASATLNDGGQQALTLPAGTYNVTQAPKTGWTFDNATCSGGNPTAITW
                     GARLTLADGASVTCAFASHQ"
     gene            complement(6815..7684)
                     /locus_tag="PK829_01900"
     CDS             complement(6815..7684)
                     /locus_tag="PK829_01900"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOU39997.1"
                     /translation="MYTTVLSAAEVAAHLGEPAWVMIDCRFDLMAPDAGEQAYRAGHI
                     PGAVYAHLDRDLSGPRRPTGEGGRHPLPAPAELTRTFGQWGIGRGTQVVAYDAQSGHY
                     AARLWWLLRFMGHEAVAVLDGGWPAWLAEGLPATTATFAPPARLFEGAPHWEWLAPLE
                     AVTAAPRLVDARAPERYRGETEPLDPVAGHIPGALNRPYTANWDAAGRWRAQEALLAE
                     FLDLLGDAPAEEAVFYCGSGVSACVNLLAMARAGLPAGRLYLGSWSEWSRLGGPVALG
                     AARGAGEDSRPSS"
     gene            complement(7826..8752)
                     /locus_tag="PK829_01905"
     CDS             complement(7826..8752)
                     /locus_tag="PK829_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU39998.1"
                     /translation="MFTKILVPLDGSALAEQAVRPAMALARAAGAEVLLLRSVASVQP
                     IFVLSSALTGQFESGGDDDGLASRQQAAHEYLTGIQRRYEQPGGVVRVVAEIGEPAAA
                     IVDVARERGVDLIVMSTHDGTGVRRAVWGSVTERVLHGADCPVLVIRSPAPIRRVVIA
                     LDGSELGERVLPPALAVARALGAQVILLRVSDAPVLNPLEISMMWDMEPSVADQDMLA
                     AARRAAGRYLDDVAARAGLSEGDTTLVLEGAPADRIDEYARLTDVDLIAMSTHGRTGL
                     RRWLYGSVTARVMRGCNRSMLIVRPPAEALRG"
     gene            9246..>9425
                     /locus_tag="PK829_01910"
     CDS             9246..>9425
                     /locus_tag="PK829_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU39999.1"
                     /translation="MLQRVLALGGAALASLLLCLLALPLVAAPWPTPSMAQPSDGLPS
                     DGLPSDGLPSDGLPSD"
     assembly_gap    9426..9525
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9526..10863
                     /locus_tag="PK829_01915"
     CDS             <9526..10863
                     /locus_tag="PK829_01915"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40000.1"
                     /translation="ATIPPLSGGAPAERPGAFVEAAPATAQTTAAPAIDLWYSATLPA
                     GHNGDPQKWVNVLGNVASAAPLASLTYRLNDGPALPLAVGPDDMRLARAGDFNIELDY
                     TDLRAGANRVVIRAADQTGAATEATVTIDYRGGGIVWQPRTYVFDWATATRIDDLAQV
                     VDGHWVLDGGGVRPTVFDFDRLLALGDLSWRDYTVTVPITVFGIDEDGYKAPSNGPGV
                     GIMVRWQGHYDAGNGASPRTGWRRLGALGWYRWQKVGENYSEGFQLLGHTGRELGTFD
                     RPLALGQTYLFKLAVQSNADPAIPATYRFKVWPATAAEPAAWDVEKRGFAGEPSGGGL
                     LLVAHHVDARFGAVRVDLATVRPRPSLTIITNGAGRGRVVTTPDGPTFRFGQDVALTG
                     APDLGSLFLGWDGAATGTTNPTQTTLFESATITATFGLGGNGIALPIIVANAP"
     gene            10867..>11382
                     /locus_tag="PK829_01920"
     CDS             10867..>11382
                     /locus_tag="PK829_01920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU40001.1"
                     /translation="MEPSEYRAIHRVEDRHWWYAGMRAITAALMESLYTGRRDLRILD
                     AGCGTGGAMSYLTRFGQVTGCDLSALALELARTRGLAALGQASVMALPFAPATFDLVT
                     SFDVLYHRAVDEPHAALRQFHRVLRPGGRVLLRLPAYNWLRGGHDVVTHTARRFTTGD
                     VAEALGGAGFSV"
BASE COUNT         1867 a         3816 c         3803 g         1796 t
ORIGIN      
        1 cgtggccgcc ggggtggtgc tgaagatgcc aatgtactgc aagttacccg gcagttcatc
       61 ggtaacggtc agctcgtcaa ttgtctgcgc gtcggcgatg ttgacgttga gttcataccg
      121 ccgcgggaag ttcggccccg tggccgtctc gtcttccggg ccgatgtaga ccttatccag
      181 cgtcatcaac gtcggcgtca gtgtctcttg cgtaaagggg gtaacaatgg ccgggtcgct
      241 gcccgggttg tcgagtgggt cgttgccata gcgaaagacg ccgcgcgact ggaagggcag
      301 agccttgtcc aggtcagcga agtcgctcat gtcggccgta atggtcacct cggccggcgg
      361 ctggccgggc gtgaaagagc caaacggcag ttgcaggctg acgaattgcc atgtaaagga
      421 cggatcgaag ccgaccggca gcgccgggca gaccaccgtc atgccaggga cgtagggatg
      481 attgatcgtc gcgccaggcg cgcacgtcag cgtggccgac gtgaccgccg tgcccaggta
      541 ggtcgcgctg atgaaactag cgccgtcatt cgggccaccc gcgctcgtgc cgtccgcgcc
      601 gccaatgggc aggaacaggt cgatgtaggg gccatagccg gcttgcgcgc cagcgccagt
      661 attgtcgaag gtggccgtga atgtaaacgg ctcgccaaga aaaggcgagt cgggcaaatc
      721 cagggtcacc gtgggcgtgg gtgctgccag ggccggggct accaatacaa tagccaacac
      781 cagcacggca gcgctgatac taaagacccg cctgagcgac gagcggcgaa aagtgggaat
      841 catgaaacgg gttctcctta tccagtacta cgtttcagta tcaaaaagca cgctaccgct
      901 aaagtagcgc gctaatactg tcctgtgaaa tgggtggccc agagttgaag taaagaaatg
      961 cctaacgaaa caatacaccg gcacattttc cggccacggg gccatgttta gggatgagac
     1021 caggcgttag ggaacagcta gctgcccgtc atcatctcaa taaggctatg gtactaaagt
     1081 accaagaaaa tgtcaagcat tttatacatg gacggggccg aatgttcaaa ggaaagggca
     1141 gcctaattga tccccgactg ttggtcaata gtcccatcag ccagcacgtc atctggccgg
     1201 ggcaatcaca cggaagccga cggtattaaa cgactgcgcc ggctcgacct ctacgcgcca
     1261 ggtgacgcgc agatgacgcg cctcgctggg ccaggccccg ccgcgaacga cgcgcgcgct
     1321 gccgccgctg ccctcggctg tcgtccactc ccagacgttg ccggccatat ccagcgcgcc
     1381 ataggggcta tcgcccgcgg gcgagaagca gcccacggcc gtcgtcgcgc cctgacccat
     1441 gccgaagttg gcccgcccgc ccgacggtgg ctcgtcgccc caggggaaga gacggccatc
     1501 gctgccgcgg gcggccttct cccactccgc ctccgacggc agccggaggc cggcccattc
     1561 gcaaaacgcc tgcgcgtcgt gccaactgac gctgaccacg ggatggtgca tgcgctcctc
     1621 gatgctgctg cgcggccctt ccggccgccg ccaataggcg cgctccacgt cttgccagcg
     1681 gccccccacc cacacgcggc cgaagccgct ggcctcggcc gtggtggtat agcccgtggc
     1741 ctccacaaag cgggcaaact gcgcgttcgt caccgcggcc cggctgatcc agtactcacc
     1801 caaccgtacc gatcgctgcg gccgctcgtg gcgcggatcg gcttcgcgcg cgccatagag
     1861 gaacgccccc gcggccacgc gcaccagctc gatgcgcgcg cgctcgtgca actgccggtc
     1921 agaaaagagg cgcatctcat cgacccggcg tttggcgggg tcgccggcgg gctggggccg
     1981 cggcggccgc ccttccagcc gggccagcag atgctccagg cccgtctgat agtcgtcgcg
     2041 gaagggcaca ttctgatagg ccgtccatag cggcggcggc cggctggggg ccacgtccag
     2101 ggggataaag cgcatgaggc gcgcgtgttc cagactgatg gctacgttgg tctccgtgtt
     2161 gacccacggc gacgccgacg ccgccggcgt cagcgccaga acgtagtagc tgctggcctc
     2221 caggccgcga ttgatggcat cgacccacgt ttcgccggga gcgatgctat ccggggcgat
     2281 ccacacgttc cagccgtggg cgcataggtc ggccgccaac cggtgggcga agccatcgtc
     2341 ctcatgggcg tggctgatga acacctggcg cgggtcacgg ttgggccggg cagggatggg
     2401 cggcacgggc ggcgggcgca ggaattcgcg ctcataggcc cgcggctgct gccgcccgag
     2461 ggcggcctcc aggtcgccca ggcggccggc ccgctccaga tggaggatca gctcgcgccc
     2521 cttgccattc ttcgtgtcgc cgctcaggtt gtcgtaatcg acgaagaggt taaaacaaag
     2581 cgtcttcagg ccgtccagcg tataatggcg gctcaggaag tcgagaagtg tgttgcggtc
     2641 ttgggccatg cgtcgcggcg ttgtgttgta tagatacaac aattgtagcc gtcgggcgac
     2701 agggcgcaag cccgtggcga tctttttaca tgtggggcga gggggcgtta acgccgttgg
     2761 ctctccacgc tattgggcgt agaatggaca cgctcgacaa gggcgcatcg cttcccgccg
     2821 caacaactgg gccggtcatc gccaggcgat ccggctacgc gtgaaagcaa ggagaaaggt
     2881 catgtccaac cgatcgattc tgcgctgcct ctttattgtt gctgtgctgg ctgtcagcgt
     2941 ggccgcggcg caagcagagc cgaatgcgcc ggcggccgtg cgcgttcgcg tcgcccacct
     3001 ggcccctttt gatggctccg acgccgcctc catcgccgtc cacgtggatg gcgccgccgt
     3061 cggcggaacg atggcctatg gcggccacag cgcctatatc accctgccgg gcgcggccgg
     3121 cgaccacctg atcgaagtgc tgcgcgatgg cgctgttgtc ctggccgagc cgcacgccct
     3181 ggccgacggc gatacatcca tcgttgtcat tggcgacgac agccaggtgc ctctggactt
     3241 gctggtgctc gacgagaatc tggccgagcc gggcgtcggc cgggccgggc tgcgcatcac
     3301 ccacgtggcc gccatcggcg cgaccattga tgatacgaag gtcgatatat gcagccagac
     3361 gggcgagttg ttcaacgcca ccgcggccgg gctgcgctat acgcgcacga catcctatcg
     3421 tctgctgcct gtcggagact atgacctgaa gatcacccgc ctgaatgccg gcggctcttg
     3481 cctcggcccg gtagtcatcg acccgccggt gctgactctg gccgagggca ccaagaccac
     3541 cctcttcctg gtgggcgacg gcacaaacca gacgctggct gtattcacct ttgccgacgg
     3601 cctgatcggc gacgataacc cgccggcgac gggcggcacg ctcttcctgc ctgtgttggc
     3661 cgcgtcgtca tagcccggcg caatgcctga ccggctgtct gggccatagc aatcccaatc
     3721 ccaggtgtaa tcccgcattt tagtagcgat ccgctacgag gaggacaaac agcatggagc
     3781 caaagcaact tgggccgatt ggcggcgagg gtgggcggcc gttcgacggc tacacgatcc
     3841 ccgacgatgc ccgcctaacg gccgtccacg tctttgccga gtgggtgctc gacgccctgc
     3901 gcctcgagtg ggtggacagc aacggcgcgc ccggcagcca cccgcccatc ggcggcctgg
     3961 gcggctatca ccacactttc tatctggacg aggatgaata tctgaccggc atcagcgggc
     4021 gctgcggctg gtacgtcgat agcgtgcgct tccacaccaa caagcgcgtc tccgatctct
     4081 tcggcggtga gggcggcgag cgcgaataca cgctcctggc ctcgccgggc gaggaggtcg
     4141 ccggcctgtt tggccgctgc gactggtata tcgacgccct gggcctccaa ctgcgcccgc
     4201 tggccgtgcg cgaggaggcc gccgaaggcg cggccctgga ggccgaagcc gaggcggaac
     4261 ggctggccga ggcggccgag gaagaactgg acgaggccct cgtcgacgcc gccgcgatgg
     4321 ctatggaagg cgaggacgtt ctgagagcca gcatcgccca ccgcagcgaa gccctgacct
     4381 ggctgcggtc tgacccggag gccatggagg ccgaggcgac gcccgcgccg gcggaagccg
     4441 atgcgctggc cgacctggtg gccgcgctgg aggaagagat cgacgctgag caacaggagc
     4501 gcgcggccga ggaggccctg gacatggccg ccggccgcgt ggccgacttc cccccggtcg
     4561 aggccagctt gctggagtgg acgggggaag agggcgaacc gctggatgcc gtcgtcgctg
     4621 tgcgccgggc ggatgtcgct tcggagctgg aagtcgaggc gctagaggaa gcggcgatcg
     4681 ccgaggcgat tgccgtgctg cacgagggcc acggcggcga gggcacggtc gatctggctg
     4741 tttacacgca ggtggacgag agcgacgaag gcgacgtggc cacagtgctg gccgtcgccg
     4801 ccccggccag cggcgaagaa ccgccggccg gcgagagcga gacagccatc atggtcgtgg
     4861 acgccatcgc ctctgaagat gaactgctgg agttggagca ggcagcggtc gaaggggcgc
     4921 tgctggccct ggaagaagac ctcggcgaag ccatcgacga cgccgatgtc gccatctata
     4981 tgggcatgac cgaggacgag acgggccagc cctatggcgc ggtggtggtg gtggcctcgc
     5041 gcagccctgt cgcgccggcg gcgaccatgc gctcggccac cgagcggggc agcttcagcg
     5101 acaaggtgat gcaattcggc ggcctggagc cgcgcccgca agacctgcaa ctggtcgagg
     5161 gcatcggccc caagatcgcc gcgctgctca tcgagaacgg cgtcaacgac ctgggccagc
     5221 tggccgagac acccgttgag cgcctgcgcg aggttctggc cggggcgggc cgtcgcttcc
     5281 ggctggccga tccggccacg tggccgcgcc aggcgcacct ggcggcgact ggccaatggg
     5341 ccgaactgac ccagttgcaa acggagttga aggcaggacg agaagagcgc taggcgggac
     5401 ggcccgtcgc gccgggcgac gggccgtccc gttggccgtc ttactggtgg ctcgcgaagg
     5461 cgcaggttac cgacgcgcca tcggccagcg ttagacgcgc gccccaggtg atcgccgtcg
     5521 ggttgccgcc gctgcacgtg gcgttgtcaa acgtccagcc tgtcttgggc gcttgcgtca
     5581 cgttgtaggt tccggccggc agcgtcaggg cctgctggcc gccgtcattc agagtggccg
     5641 aagcctgggc gtagatctgg atacggttgg cgtcgagatc agtggcataa acccgattgc
     5701 ccacgacgag cagcccgttc ggccgggaga tggccgcgcc gccggcctcg ctccagtggg
     5761 caatatagac gccgttggtc gtcagcttct ggatgcgatt gccgaaggcc tcactcacgt
     5821 agagatagcc gtccgccccg acctccacgc cgacggggac gcggaattgc cccggccctg
     5881 tgcccgagac gccaaacttg cgttggaaga caccatcggt gttgtagacg acgatgcgat
     5941 tgttgttgga atcggccacg tagaggttgc ccgcgccgtc gaagtccagg tgggccggga
     6001 aattcaactg accgtcgccc gcgccctggg aaccccattg gcgcaggaaa gtcccttcct
     6061 gggtgaagac ctggatgcga tggttgaacg tgtccgacac atagacgttg ttatccgggc
     6121 caatggcgac gccgttgggc atgttgaact tgccgttggt tgtgccctgg ccgccccact
     6181 cgcgcaccag cgcgccgctg gtcgtgttgt agacagtgat gcggtggttt tcggtgttgg
     6241 ccacgaacag caggttgcca ctgatggcaa tggcattggg ccgcagcagc cggcccgcgc
     6301 cgcgccccga ccggccgatc ttctcgataa aaacgccggt ggcgctgagc ttctggatgc
     6361 ggctgttacg gtggtcgctg acgtagatat tgccggcggc gtcctgatct acgtcccgcg
     6421 gctggcgata ctggccgttg cccttgccaa accggcccca cgaaccctga aaggaaacgg
     6481 ccgtcagcca gaagccctgc ccgccggcgg gggtcgtggt cacgccaaga gtcagggttg
     6541 ccgccgtcgc cgccgctggc gcgtcctggg cggagggcgc atcctgcgcc gcgacggccg
     6601 acgccagcaa cagggccagg agcagcaccc ccatgatcat caggccacgg acaaaacggt
     6661 tcttgctttg ggtctccatt cgtattctcc ttgactgcgt tgtgtggcca agctcgttcg
     6721 tgcgcatcgc cacgaacccc tactgtggtc gccgcgcatc cgcttgaatg gcagcagtgt
     6781 aacatactgc cacagtctac cgcaataggg taggtcagga agacgggcgg ctgtcctctc
     6841 cggctccgcg cgccgcgcca agcgccacgg ggccgcccag ccggctccac tcactccacg
     6901 agccgagata gaggcgaccg gccggcagcc cggcgcgggc catggccagc agattgacgc
     6961 aggccgagac gccggaaccg caatagaaca cagcctcctc cgcgggcgcg tcgcccagca
     7021 ggtccaggaa ctcggccagc agcgcctcct gcgcccgcca acggccggcg gcgtcccagt
     7081 tggcggtgta gggccggttc agcgcgccgg ggatgtggcc cgccaccggg tcgagcggct
     7141 ccgtctcgcc gcggtagcgc tccggcgcgc gggcgtccac caaccgcggc gcggcggtga
     7201 cggcttccag aggcgccagc cattcccaat ggggcgcgcc ctcgaaaagg cgggctggcg
     7261 gcgcgaatgt cgctgtcgtt gccggcagcc cctcggccag ccaggcgggc cagccgccgt
     7321 caagcacggc cacggcttcg tgccccatga agcgcagcag ccaccacaat cgggcggcgt
     7381 agtggccgct ctgggcgtcg taggccacga cctgcgtccc gcggccgatg ccccattggc
     7441 cgaaggtgcg cgtcaactcg gctggcgcgg gcaacgggtg gcggccgccc tcgcccgtgg
     7501 gccggcgtgg gccggacaga tcgcggtcca ggtgggcata gaccgcgccg gggatatggc
     7561 cggcccggta ggcttgttcc ccagcgtcgg gggccatcag gtcgaaccgg caatcgatca
     7621 tgacccaggc aggctcgccc aggtgggcgg ctacctcggc cgccgagagg acagtggtat
     7681 acatcgcgct catggcccga tcatagcacg aaaagcgccg tgcctgaatg ggagcggagg
     7741 catggcccac agatgacgcc gatcatgcca attgacgccg atgggaatcc ctgccggcgt
     7801 catcagcgtc ctatggcccg cccgctcagc cgcgcagcgc ttcagccggc ggccggacaa
     7861 tgagcatgga gcgattgcag ccgcgcatca cccgggcggt cacgctgccg tagagccagc
     7921 gccgcagccc ggtgcggccg tgggtgctca tggcgatcag gtcgacgtcg gtcagccggg
     7981 catactcgtc gatgcggtcg gccggcgcgc cctccagaac cagcgtcgta tcaccctccg
     8041 acagacccgc ccgcgcggcg acgtcgtcca gatagcgccc ggccgcccgc cgcgccgcgg
     8101 ccagcatatc ctggtcggcc acggacggct ccatatccca catcatgctg atctccagcg
     8161 ggttcagcac cggcgcgtcg ctgacacgca acaggatcac ctgcgccccc aacgcgcgcg
     8221 cgacggccag cgccggcggc aagacgcgct cgcccagttc ggagccgtcc agcgcaatca
     8281 ccacgcgccg gataggagcg ggcgagcgta tcaccagcac cgggcagtcc gcgccgtgaa
     8341 gcacgcgctc ggtgacactt ccccagaccg cgcgccgcac gcccgtcccg tcgtgggtgc
     8401 tcatgacgat cagatcgacg ccccgctcgc gggccacatc gacgatggcc gccgccggtt
     8461 cgcctatctc ggccaccacg cgaaccacgc cacccggctg ttcgtagcgc cgttggatac
     8521 cggtcaggta ctcgtgggcc gcctgctggc ggctggccag cccatcatcg tccccgccac
     8581 tctcgaactg ccctgttaac gctgacgaca gcacaaaaat gggctgcacg ctggcgacgc
     8641 tgcgcaataa caacacctct gcgccggccg cccgcgccag cgccatcgcc ggcctgacgg
     8701 cctgctcggc caacgccgaa ccatccaacg gaacgaggat cttcgtgaac atagcgcctc
     8761 ccaccggctt gctcatgcag ccggcgcaat ggccctgggg cgctcgcgcc gctcgtccgg
     8821 ctgcttacga gcgtcagata gacgccccca tgtcatcact atagcgtaag cgagccgggc
     8881 ggcgatagtg acggaaatca ccccaccgat atgacatttg tcacgccggc cgctccggcg
     8941 ctagacctct caggtcttag agaaatacga agccctggcc catatcatcc gccttttatc
     9001 ctctttatag agcaatgagc taagtggcag ggctttgcca cagtcacccc aggcgcgccg
     9061 acctgaccgg tgggcggcca gtcgttctgt ggtggcagac tatgcgtggc cgcccaccgg
     9121 tcaggtcaat gatcgctcga cgactgtggt aaagccctga actaagtggt tctgcccttg
     9181 cccacacgcc ggctggcggc taaactttcg tgccgcttcg gagccgacgc ggccaagagg
     9241 atagaatgct acaacgagtc ttagccctgg gcggcgccgc gttggcgtcg cttctcctgt
     9301 gtctgttggc gttgcccctg gtggccgcgc cctggccaac gccgtcgatg gcccagccgt
     9361 ccgacggtct gccgtccgac ggtctgccgt ccgacggtct gccgtccgac ggtctgccgt
     9421 ccgacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccac tattccaccg
     9541 ctgtctggcg gcgcgccggc cgagcgcccg ggagcgttcg tcgaggccgc gccggccacc
     9601 gcgcagacga cggccgctcc ggccattgac ctctggtaca gcgcgacgct gcccgccggc
     9661 cacaatggcg acccgcaaaa gtgggtgaac gtgctcggca atgtggcttc ggccgcgccg
     9721 ctggccagtc tcacctatcg cctcaacgac gggccggcgc tgccgctggc cgtcggcccc
     9781 gacgatatgc gcctggcccg cgctggcgat ttcaacatcg aacttgacta taccgacctg
     9841 cgcgccgggg ccaatcgggt tgtcatccgc gccgccgacc agacaggcgc cgcgacggag
     9901 gcgacagtga ccattgacta ccgcggcggg ggcatcgtct ggcagccgcg gacttacgtg
     9961 ttcgactggg ccacggcgac gcgcattgat gatctggcgc aggttgttga cggccactgg
    10021 gtcctggacg gcgggggcgt gcggccgacc gttttcgact tcgaccgcct cctggcgctg
    10081 ggcgatctct cctggcgcga ctacaccgtc accgtgccca tcaccgtctt cggcattgac
    10141 gaggacggct acaaggctcc cagcaacggg cctggcgtcg gcatcatggt gcgttggcag
    10201 gggcactacg acgccggcaa cggcgcttcg ccgcgcaccg gctggcggcg gctgggcgcg
    10261 ttgggctggt accgctggca aaaagtgggc gagaactaca gcgagggctt ccaattgttg
    10321 ggccacaccg gccgcgagct gggcacgttc gaccgcccgt tggctctggg acagacctat
    10381 ctgttcaagc tggccgtcca atccaacgcc gacccggcca tcccggccac ctatcgcttc
    10441 aaggtctggc cggcgacagc ggccgagccg gcggcctggg atgtggagaa gcgcggcttc
    10501 gccggagaac caagtggcgg cgggctgctg ctggtggccc accacgtcga cgcccgcttc
    10561 ggtgcggtgc gggttgacct ggctaccgtc cggccgcggc cgtcgttgac catcatcacc
    10621 aacggcgcgg gccgcggacg ggtcgtcacg acgcccgacg gccccacctt tcgctttggc
    10681 caggacgtgg cgctgaccgg cgcgcccgat ctcggctcgc tcttcctggg ctgggacggc
    10741 gcggccacgg gcacgaccaa ccccactcag acgacgctgt tcgagagcgc caccatcacg
    10801 gctactttcg gcctgggcgg caacggcatc gccctgccga tcattgtggc caacgcgccc
    10861 taggccatgg aaccctcaga gtaccgcgcc atccaccgcg tcgaagaccg ccactggtgg
    10921 tatgccggta tgcgcgccat caccgcggcg ctcatggagt cgctctacac cggccgccgc
    10981 gatctgcgca tcctggacgc tggctgcggc acaggcgggg ccatgagcta cctgacccgg
    11041 ttcggtcagg tcaccggctg cgacctgtcg gcgctggcgc tggaactggc gcggacgcgc
    11101 ggattggcgg cactggggca ggcgtcggtg atggccctgc ccttcgcccc ggcgacgttc
    11161 gacctggtga ccagtttcga cgttctttac caccgcgctg tggacgagcc gcacgcggcc
    11221 ctgcgccagt tccaccgcgt gctgcggccg ggcgggcggg tgctactgcg gttgccggcc
    11281 tataactggc tgcgcggcgg ccacgacgtc gtcacccaca cggcgcgccg cttcaccacc
    11341 ggcgacgtgg ccgaggcgtt gggaggcgcg ggcttctccg tc
//
LOCUS       DAOSMQ010000031        11220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5777_length_11220_cov_5.006090, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000031 DAOSMQ010000000
VERSION     DAOSMQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5777_length_11220_cov_5.006090"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK829_01925"
     CDS             complement(<1..367)
                     /locus_tag="PK829_01925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU40002.1"
                     /translation="MALPLVTVIMPVRNEAAYIERSLGAVLAQDYPAERLEILVVDGL
                     STDGTREMVGRMARPPRVRLLDNPAGIVPPGLNIGIAQARGEIVVRVDGHCEIAPDYV
                     RHCVAHLLAGHEGTPIDAVG"
     gene            complement(436..1725)
                     /locus_tag="PK829_01930"
     CDS             complement(436..1725)
                     /locus_tag="PK829_01930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU40003.1"
                     /translation="MRRHDLAAVAALFVLTLLVAGVFWALLPAEYRQNQSTDYTIHYE
                     PVARAILAGRGIVDETGALATRYPPGFSLLLAGAWGLGRAAGVADDTALLAFRLLCAA
                     LSTVLLYALARLVWPLRAALLVGAAWACYPFFLWITKQPNSEVPFLPVFFATLFVFWL
                     AVLRRPRAWPLYVLAGLLAGAAMLIRPAALGLGGVMAVLVLLLARPLRWPARLGLAAL
                     LVLGNVLAVAPWLGAVYARTGTFIPLSTGGTLTLKDGLTFLVDEKNYRRDVPLADDVA
                     ALLLTFRDRRAEMSSTGDVVAIVLDEARQAPVAFLKFTWIKATRSWYGNDSRTFEGPT
                     LLAQVAYALLILWGNAYAFFARGTPAVARPADLRRMLAGNWLIVVAFWAMTMTAVPLL
                     RYMLPLMGPLFIAVPGVYFSLRALRAGAWAGPTPSLE"
     gene            complement(1755..3140)
                     /locus_tag="PK829_01935"
     CDS             complement(1755..3140)
                     /locus_tag="PK829_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU40004.1"
                     /translation="MPDWLKVYHQLPYPAKVLAASARGRRLERWRYGPDTERLVDEVL
                     ARDDWTVAQWDAWREERLAFVLHRAATRVPYYRDQWARRRAAGDRAAPDVLANWPALS
                     KDALRAQPHAFLADDCDPATMYYEHTSGTTGKPLHLWHSRATLNAWYALTEARMRRWY
                     GLSREDRWAILGGQLVAPVERDRPPFWVWNFALRQLYLSAYHLTPDNIAAYLEAMRRH
                     EVVYLLGYPSGLYTLARVALERGLDAPQLRAVIGNAEPLLAHQRETIAAAFGCPARET
                     YGMAEYAAAASDCEHGRLHLWPEAGHIELLADDGDAAVPPGTPGRLVATALLNADMPL
                     IRYETGDRAALATEPCACGRGLPVLAAIEGRVDDMIVTPDGRHIGRLDPVFKADLPIH
                     EAQIIQTTPDTLRVLYVPTAAYTPGDGEALIGRIHDRVGPAMRVVLEAVPAIPRTANG
                     KFRAVISEVNR"
     gene            complement(3133..4257)
                     /locus_tag="PK829_01940"
     CDS             complement(3133..4257)
                     /locus_tag="PK829_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU40005.1"
                     /translation="MDTLDTRSHIPAVEPFAPAPLRPRLCFAGPLLGINSGWVTSQGE
                     IVAGLLANEGFVVRQTSHIPARLPRLADMLRSLVAWRNDIDVVIHQVFSGAAFVVADL
                     TSALCQALGLRQVFALHGGTLPQFAAAHPGWTRRVLARADRVVAPSSYLIERMVRPLF
                     PAGGRAGRPANWARVIPNILALDQYTYRHRAVVAPRLLWMRTFHEVYNPEMAVDVLAD
                     VRQTHPQATLSMGGQEKGLHAAVVASAAARGLAGAVAFPGFLDAAGKAREFAAHDIYL
                     NTNRVDNTPVSVLEAAAFGLPIVATNVGGIPHLLRDGETGLLVPNEDAAAMANAVRRL
                     LDEPALAARLSANGRRLAESCAWDAVKGQWAALFDEVYHA"
     gene            complement(4238..5485)
                     /locus_tag="PK829_01945"
     CDS             complement(4238..5485)
                     /locus_tag="PK829_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU40006.1"
                     /translation="MRLLIVSHTPHYRAAGGFVGWGPTVRELDYLAELFDEVVHVAPL
                     YDEAAPGSALAYASPRLRLRPVAPAGGETLRAKLDIARLYPAYAVAIGDEMRRADAVH
                     VRCPANISLLALALLRAARQPGPRWVKYAGNWQPDGGEPWSYALQRRWLAANVHRGVV
                     TVSGRWPDQPAHVHSFYNPSLSEDELVAGRVAATAKQLSSPLELLFVGALNDGKGAGR
                     ALAVAEALQRQGVPYRLRLLGDGPDRPRYEAWAAERGLREVIFHGWVSREAVGRFDAT
                     AHFILLPSQTEGWPKVLSEAMAYGAVPITSAVSSIPQILAETGAGVARPVGDIEGMAA
                     AIVNYVNQPDEWLAASRAGAAVAAQFGYRAYQDAVRALFAGAWGIALAAPMTPSAPVS
                     HAVSHRVMAAVMPHPSTNGHTRH"
     gene            complement(5508..6692)
                     /locus_tag="PK829_01950"
     CDS             complement(5508..6692)
                     /locus_tag="PK829_01950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU40007.1"
                     /translation="MSAGPRLLLLSSEFPPGPGGIGMHAYQLARHLAAGGWLVTVATP
                     QAYVAAAARADFNRRQPFAVITLPERDAGPLWPVARLRALAGAMGAARPDILLASGRR
                     ALWTAALLGPLYRRPYVAVGHGSEFHSGSALPRVLTGRSIAAAAAVVAVSEYTAGLVR
                     AAGRPRELVVIPNAADGERFRPGQDTAAPRRELALDGRRVLLTVGRVGARKAQDVVIR
                     ALPRVLAVHPDVVYVMAGLPEKRAEFTALAESLGVAAALRFTGLVEDALLPALYDLAD
                     VFVLVSRRTAAGDVEGYGIVVQEAALCGTPAVVSRGCGLTEAIREGETGVSVPPDDPA
                     ATAAAIISLLDDAAAGGDMGARARELATRATWAERAADYDDLLRRFLPQGTGHRPLAT
                     GH"
     gene            complement(6689..8149)
                     /locus_tag="PK829_01955"
     CDS             complement(6689..8149)
                     /locus_tag="PK829_01955"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU40008.1"
                     /translation="MITRYSPEPPTSSIGRSIGQPENAARELAGSVNVRIALFLLVHM
                     PLAYAMEAFWPLATAHAVLVLLAGIRWAWLGRTRQVLYCLSYIAGFEVLWRMTEARVF
                     WEYGKYALALVVLMALFAEWRRSDARLRTVLPVILLAAMVPAVALAVLQFSPAEAFDQ
                     LSFNLSAYLALGAAALYCWERPVDRPTAANLLLALMAPIVGVLFLASFSTITQIGVDT
                     FATASSWIRSGDFGANQVSNVLSLGALAGVILLIILPRAQGARLLIAGLTVAMVIQGI
                     LTFSRGGLYSLILAGLAFGLNLLTTPGARGRFLLLIAVFIALVFGVIYPWLNDLSTGA
                     VTARFEDLDTTGRLELAQADLMAFQDSPLLGVGVGGSMPYHVYVFGEDVGTHTEYTRL
                     LAEHGLFGILVMALMALMLLKRYLGNAPGLGRAISAAMAIWTLSVMAHSATRIVVIPY
                     ALVLAFLAWRLDEGHAPADADGLSAPATAVAPTRVR"
     gene            complement(8146..>9875)
                     /locus_tag="PK829_01960"
     CDS             complement(8146..>9875)
                     /locus_tag="PK829_01960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOU40009.1"
                     /translation="PAPAWPLGETRLVPATVAAIVSAAAALAPPAPPSPRSPAALLFW
                     DAALGQPDADLVAALLAGPGDVWHAGLRLGMAGLPALIDYVDPVWRFNRDPNPAAPGV
                     SWRLSLRACLMRADVVRCLGGPAAGFDTLAGAALELGHRWVTGGAMLRYVPDLVAAPT
                     GAVDAPTPADEFRFVRRRYGRMWAAWAAWRAARHGAGLLATARAFRAARPAAGAGPAG
                     TLHPVELPTAVGADAPTVSVLIPTLDRYPHLFVLLDHLRAQTVSPLEIIVVDQTEAAA
                     RDLAWPARFADLPLRVIRRDTAGQCSSRNAGLAIVRGDAVLFLDDDDEAPPDLIARHL
                     AYLARFGVDASCGVAEELGAGALPAAFRRVRESDVFPTNNALLRTAALADAGLFDLAY
                     ERGERADGDLGMRLYLAGKTLGLNPAASVVHLHAPRGGLRQHRARVMTRGGSRGSLRQ
                     RHLLAPTEGYLWSRYFTPRQVHEALLIRTVATLRGEGRRPRRLLRAALMTLLLPDTWR
                     QNRSRLAEGQALLARFPSIPAYHPNPLSPGERAESPLPQGRASERAESSLPQGEGQGE
                     GQKGAFVLP"
     assembly_gap    9876..9975
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9976..11150)
                     /locus_tag="PK829_01965"
     CDS             complement(<9976..11150)
                     /locus_tag="PK829_01965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU40010.1"
                     /translation="MRLVLVVPRFPRLSETFIVSKFVGLADAGWDVHVVCAEAEAWTA
                     FPALLARPHLRRRVHRQWPHAAAWQVALLWLPALLLTLLRAPRATLRYWRRALPGGLR
                     RAARDFYLDAAIIALSPDILHFEFGALAVGRTALKQRLGCRLTVSFRGYDLNYVGLDD
                     PGHYAAIWRDADAIHTLGGDLWRRARRRGCPADMPHALIPPAVDAEVFRPAARDDDAI
                     GPGRPLRVLSVGRLEWKKGYEVGLEAVRLLKARGVPVHYRILGGGQYLEALAFARHQM
                     GLAAEVEFLGARPPADVPAQMQWADVLLHPAVSEGFGNAVLEAQAMALPVVCSDADGL
                     AENVADGVSGFVVPRREPAALATALARLAADTELRAQMGAAGRERVQTCFRPEAQIAA
                     "
BASE COUNT         1836 a         3969 c         3979 g         1336 t
ORIGIN      
        1 cgccgacggc gtcgatgggc gtgccctcat gcccggccag caggtgcgcc acacagtggc
       61 gcacgtagtc cggggcgatc tcgcagtggc cgtccacgcg cacgacgatc tccccccgcg
      121 cctgggcaat gccgatgttc agccccggcg ggacgatgcc ggcgggattg tccaacaggc
      181 gcacgcgcgg cggccgggcc atacggccaa ccatctcgcg cgtgccatcg gtcgataggc
      241 catcgacgac gaggatttcc agccgttcgg cggggtaatc ctgggccaac accgcgccca
      301 gactgcgctc gatgtaggcc gcctcgttgc gcacaggcat gatcacagtg acaaggggaa
      361 gcgccatgtc gttagtgtaa cgccgccgcc gccctcctcc cgtgcagaag gcgtgaacgc
      421 ctcgtgagga acgggttatt ccagcgacgg cgttgggccg gcccacgcgc cggctcgcag
      481 cgcccgcagc gagaagtaga cgcccggcac ggcgatgaac aacggcccca tgagcggcag
      541 catgtagcgc aagagaggca cggccgtcat cgtcatggcc cagaaggcga ccacgatgag
      601 ccagttgccg gctaacatgc gccgtagatc ggccgggcgg gccacggccg gcgttccccg
      661 cgcgaagaag gcataagcgt tgccccagag gatgagcagc gcgtaggcca cctgcgcaag
      721 cagcgtcggc ccttcgaagg tgcggctgtc gttgccatac cagctgcgcg tggccttgat
      781 ccaggtgaac ttgaggaagg ccaccggcgc ctgccgcgcc tcgtcgagga cgatggccac
      841 cacgtcgccg gtcgaagaca tctcggcgcg acggtcgcgg aaggtgagca gcagcgcggc
      901 gacatcgtcg gccagcggta cgtcgcgccg gtagttcttc tcgtccacca ggaaggtcaa
      961 cccgtccttg agggtcagcg tgccgccagt gctgaggggg atgaacgtcc ccgttcgggc
     1021 gtaaacagcc cccagccacg gagcaacggc caggacgttg cccagcacca gcagcgccgc
     1081 cagccccaac cgcgccggcc agcgcaacgg ccgggccagc aatagcacca gcacggccat
     1141 caccccgccc aaccccaacg ccgccggccg gatgagcatg gccgccccgg ccagcagccc
     1201 ggccagcacg tagagcggcc aggcccgcgg ccgccgcaag accgccagcc agaagacaaa
     1261 tagcgtggcg aagaacaccg gcagaaacgg cacttcgcta ttgggctgct tggtgatcca
     1321 caggaagaag gggtagcaag cccacgccgc gccaaccagc agcgccgccc gcagcggcca
     1381 gaccagccgc gccagcgcgt agagcagcac cgtcgaaagt gccgcgcaca gcaaacggaa
     1441 ggccagcagc gccgtgtcgt cggccacgcc ggcggcgcgg cccaggcccc acgcgccggc
     1501 cagcagcagc gagaagccgg gcgggtagcg cgtcgccagc gcgccggtct catcgacgat
     1561 gccccggccg gccagaatcg cccgcgctac cggctcataa tggatcgtgt agtcggtgct
     1621 ctggttctgg cgatactcgg ccggcagcag cgcccagaag acgccggcca caagcagggt
     1681 cagcacaaag agcgccgcga cggcggcgag atcatgtcgt ctcatggttc gctccaggga
     1741 aggcgtggct ggcttcatcg gttgacctcc gagatgacgg cgcggaactt gccgttggcc
     1801 gtgcgcggga tggccgggac ggcctccagg acgacacgca tcgccgggcc gacacggtcg
     1861 tggatgcggc cgatgagcgc ctcaccgtcg cccggcgtgt aggccgccgt gggcacgtag
     1921 aggacgcgca gtgtgtccgg cgtcgtttgg atgatctgtg cctcgtggat gggcaaatcg
     1981 gccttgaaaa ccgggtcgag ccggccgatg tgacggccgt cgggcgtgac gatcatgtca
     2041 tccacccgcc cctcgatggc cgccaggacg ggcaggccgc gcccacaggc gcatggctcc
     2101 gtcgccagcg ccgcccggtc gccggtctcg tagcgaatca gaggcatgtc ggcgttgagc
     2161 agggccgtgg ccaccagccg gccgggcgtg cccgggggca cggccgcgtc cccgtcgtcg
     2221 gccagcaatt cgatgtggcc ggcctccggc cagaggtgca gccggccgtg ttcgcaatcg
     2281 ctggccgcgg cggcgtactc ggccatgccg taggtctcgc gcgccgggca gccgaacgcc
     2341 gccgcgatcg tctcgcgctg gtgggccagc aacggctcgg cattgccgat caccgcccgc
     2401 agctgcggcg cgtccagccc acgctccaaa gccacccgcg ccagcgtata cagccccgat
     2461 gggtagccga gcaaatagac gacctcgtgc cggcgcatgg cttccaggta ggcggcgatg
     2521 ttgtccggag tcaggtggta ggccgacagg tagagttgcc gcagagcgaa gttccacacc
     2581 cagaaaggcg gccggtcgcg ctcgaccggg gcaacgagtt gcccgcccag gatggcccag
     2641 cgatcctcgc ggctcaggcc ataccagcgc cgcatgcgcg cctcggtcag cgcgtaccag
     2701 gcgttgagcg tcgcgcgact gtgccagaga tgcagcggct tgccggtcgt gccactggtg
     2761 tgctcgtagt acatcgtcgc cgggtcgcag tcgtcggcca ggaaggcgtg gggctgggcg
     2821 cgcagggcgt ccttgctcag cgccggccag ttggccagca cgtccggcgc ggcccggtcg
     2881 ccggccgccc gacggcgcgc ccactggtcg cggtagtaag gcacgcgcgt cgccgcgcgg
     2941 tggagcacaa aggccagccg ctcctcgcgc caggcgtccc actgcgccac cgtccagtcg
     3001 tcgcgggcca gcacctcgtc aacaagcctc tcggtgtcgg ggccatagcg ccagcgctcc
     3061 agccgccgcc cgcgggcgct ggcggccagc accttggccg gatagggtag ctgatgatag
     3121 accttgagcc aatcaggcat gatacacctc gtcgaacagc gccgcccact ggcccttcac
     3181 cgcgtcccag gcgcatgact cggccagccg ccggccgttg gccgaaagcc tggccgccag
     3241 cgccggctca tccagcagcc gccgcaccgc attggccatg gccgcggcgt cctcgttggg
     3301 caccagcagg ccggtctcgc cgtcgcgcag cagatggggg atgccgccca cgttggtggc
     3361 cacgatgggc aggccaaagg ccgccgcttc caatacgctg acgggcgtat tatcgacacg
     3421 gttagtgttc aggtagatgt cgtgggcggc gaactcgcgc gccttgccgg cggcgtcgag
     3481 aaagccggga aaagccaccg ccccggccaa cccccgcgcc gcggcggagg cgacaacggc
     3541 cgcgtgcagc cccttctcct gcccgcccat gctcagcgtc gcctgcgggt gcgtctggcg
     3601 cacatcggcc agcacgtcaa cggccatctc tggattataa acctcgtgga aggtgcgcat
     3661 ccacagcagc cgcggggcga cgacggcgcg gtggcgatag gtatactggt caagcgccag
     3721 gatgttggga atgacccgcg cccagttggc gggccggccg gcccgcccgc ccgccggaaa
     3781 gagcggtcgc accatccgct cgatcaggta gctggacggg gccacgaccc ggtcggcccg
     3841 ggccaacacc cgccgcgtcc agccgggatg cgccgcggcg aactgcggca acgtgccacc
     3901 gtgcagggcg aatacctgcc gtagccccag ggcctggcac agcgcgctgg tcaggtcggc
     3961 cacgacaaac gccgcgccgc tgaagacttg atggatgacc acgtcgatgt cgttgcgcca
     4021 ggccaccagc gagcgcaaca tgtcagccaa ccgcggcagc cgggccggga tgtgggaagt
     4081 ctggcggacg acaaaacctt cgttggccag cagcccggcg acgatctcgc cctgactggt
     4141 cacccagccg gaattgatgc cgagcagcgg cccggcgaaa cacaggcgcg ggcgaagcgg
     4201 cgctggcgcg aacggctcca cggcggggat atgcgaccta gtgtcgagtg tgtccattgg
     4261 ttgaaggatg cggcatgacg gccgccataa ctcggtgcga cacggcatgt gaaaccggcg
     4321 cagagggcgt catcggcgcg gccagagcga tgccccacgc cccggcgaag agggcgcgga
     4381 cggcatcctg gtaagcgcgg tagccgaact gtgccgccac ggccgccccg gcgcggctgg
     4441 cggccagcca ctcatcgggc tgattgacat aattcacgat agccgcggcc atgccctcaa
     4501 tgtcgccaac cggccgggcc acgcctgccc cggtctcggc caggatctgg ggaatgctgg
     4561 agacggcgct ggtgatgggc acggccccat aggccattgc ctcgctgagc accttgggcc
     4621 agccctcggt ttgcgacggc agcaagatga agtgcgccgt ggcgtcgaac cggccgaccg
     4681 cctcgcgcga gacccagccg tggaagatga cctcgcgcaa gccccgctcg gccgcccagg
     4741 cttcgtagcg cggccgatcc gggccgtcgc ccagcagacg cagccggtaa ggcacgcctt
     4801 gccgttgcag cgcctcggcc actgccagcg cccgccccgc ccccttgccg tcgttgagcg
     4861 cgccgacgaa cagcagctcc agcggtgacg agagctgttt ggccgtcgcg gccacgcggc
     4921 cggcgactaa ctcgtcctcg ctcagcgacg gattatagaa ggagtgaacg tgggcgggct
     4981 ggtcgggcca gcggccgctg accgtcacca cgccacggtg gacgttggcc gccagccagc
     5041 gccgttgcag ggcataggac cacggctcgc cgccgtcggg ctgccagttg ccggcatact
     5101 tcacccagcg cggccccggc tgccgcgccg cccgcaacag ggccaacgcc agcaggctga
     5161 tgttggccgg gcagcgcacg tggacggcgt cggcacggcg catctcgtcg ccgatggcga
     5221 cggcgtaggc cgggtagagc cgggcgatat cgagcttggc gcgcagcgtc tcgccgccgg
     5281 ccggagccac cgggcgcaag cgcagccgcg gcgaggcgta ggccagggcg ctgcccggcg
     5341 cggcctcgtc gtagagcggg gcgacgtgga cgacctcgtc gaacaactcg gccaggtagt
     5401 caagctcgcg cacggtcggc ccccagccaa cgaagccgcc ggcggcgcga taatggggcg
     5461 tgtgggagac gatcaggagg cgcatagtag tggtcagtgg ccagtggtca gtggccggtg
     5521 gccagtggtc ggtggccagt cccctgtgga aggaagcggc gcaggaggtc gtcgtagtcg
     5581 gcggcgcgct cggcccaggt ggcgcgggtg gccagctcgc gggcgcgcgc gcccatgtcg
     5641 ccgccggcgg cggcatcatc caggaggcta atgatggccg cggccgtcgc ggccgggtcg
     5701 tcgggcggca cgctgacgcc cgtctcgccc tcgcgaatgg cctcggtcag gccacagccg
     5761 cggctgacca cggccggcgt gccacacagc gccgcctcct ggacgacgat gccgtagccc
     5821 tccacgtcgc ccgcggccgt ccggcggctg accaggacaa agacatcggc caggtcatag
     5881 agcgccggca acagcgcgtc ctcgaccagg ccggtgaagc gcaacgccgc ggccacgccc
     5941 agcgactcgg ccagagcggt gaactcggcg cgcttctccg gcagcccggc catgacgtag
     6001 acgacgtcgg gatggacggc cagcacgcgc ggcaaggcgc gaatgacgac atcctgcgcc
     6061 ttgcgcgcgc cgacgcggcc gacggtcagc aacacgcgcc gcccgtccag agccaattcg
     6121 cggcgcggcg cagccgtgtc ctggccgggg cggaagcgct cgccgtcggc ggcgttaggg
     6181 atgacgacca attcgcgcgg gcggccggcg gcgcgcacca ggccagcagt gtactcgctg
     6241 acggccacga cggccgccgc cgcggcgatg ctccggccgg tgaggacgcg cggcagcgcc
     6301 gagccgctat gaaactcgct gccgtggccc acagccacgt agggccgccg gtagagcggc
     6361 cccagcaggg ccgccgtcca cagcgcccgc cggccgctgg ccaggagaat gtcgggccgg
     6421 gcggcgccca tcgccccggc caacgcccgc agccgggcga ccggccacag cggcccggcg
     6481 tcgcgctccg gcagggtaat gaccgcgaag ggctgccgcc ggttgaagtc ggcgcgggcg
     6541 gccgcagcga cgtaggcctg tggcgtggcc accgtcacca accagccgcc cgccgccaga
     6601 tggcgggcca gctggtaggc atgcatgccg atgccgcccg gccccggcgg gaattcgctg
     6661 ctcagcagca gcaggcgcgg cccagcgctc atcgcaccct cgtcggcgcg acagccgtcg
     6721 ccggagccga cagaccatcg gcgtccgccg gagcatgccc ttcgtcgagc cgccaggcca
     6781 ggaaggccag caccagcgcg taagggatga ccacgatgcg ggtggcgcta tgggccatca
     6841 ccgacagcgt ccagatggcc atcgccgccg agatagcccg cccgaggccc ggcgcgttgc
     6901 ccagatagcg tttcagcagc atcagagcca tcagggccat aacgaggatg ccgaacaggc
     6961 cgtgctcggc cagcaggcgg gtgtattcag tatgcgtgcc gacatcctcc ccgaagacgt
     7021 aaacgtgata gggcatcgag ccgccgacgc caacgcccag cagcgggcta tcctggaagg
     7081 ccatcaggtc ggcctgggcc aattccagcc ggccggtcgt atccaggtct tcaaaccgcg
     7141 ccgtcaccgc gccggtggac agatcgttca accacgggta aatgacgccg aagacgaggg
     7201 caatgaagac ggcgatgagc aacaggaagc gcccgcgcgc gcccggcgtc gtcagcaggt
     7261 tcaggccaaa ggccagcccg gccagaatga ggctgtagag gccgccgcgc gagaaggtca
     7321 ggatgccctg gataaccata gcgaccgtta gcccggcgat gagcagccgc gcgccctgtg
     7381 cccgcggcag gatgatgagc aggatgacgc cggctaacgc gcccaggctg agcacattgg
     7441 agacctgatt ggcgccaaag tcgccgctgc ggatccagct tgaggcggtg gcgaaggtat
     7501 cgacgccgat ctgggtgata gtgctgaaac tggctaagaa gaggacgccg acaatgggcg
     7561 ccatcagcgc cagcaacaga ttggcggccg tgggtcgatc gaccggccgc tcccagcagt
     7621 agagcgctgc cgcgcccagg gcgaggtagg ccgacaggtt gaagctcagt tggtcgaaag
     7681 cttcggccgg gctgaactgg agcacggcca gggccacggc cggtaccatc gccgccagca
     7741 ggatgaccgg cagcaccgtg cgcagtcgcg cgtcgctgcg ccgccactcg gcgaacagag
     7801 ccatcaggac gaccagcgcg agggcgtact tgccgtattc ccagaacaca cgcgcctccg
     7861 tcatgcgcca caagacctcg aagccggcga tgtagctgag gcagtagagc acctgacgtg
     7921 tgcggccgag ccacgcccag cggatgcccg ccaacaagac caacacggcg tgggccgtcg
     7981 ccagcggcca gaacgcctcc atggcatagg ccagcggcat atggaccagc agaaagaggg
     8041 cgatgcgcac gttgactgag ccggccagtt cgcgggcggc gttttccggc tgcccaatgg
     8101 agcggccgat ggacgaggta ggtggttcgg gtgagtaacg tgtgatcatg gcagtacaaa
     8161 agctcctttt tggccctctc cctggccctc tccctggggg agagaggatt cggctctctc
     8221 gctcgccctg ccctggggga gaggggattc ggctctctcg ccgggggaga ggggattggg
     8281 gtggtaagcc ggaatggacg ggaaacgcgc cagcagcgct tgcccctcgg ccagacggct
     8341 gcggttctgt cgccacgtat cgggcaacaa cagcgtcatc agcgccgccc gcaagaggcg
     8401 ccgcggccgg cggccctcgc cgcgcagcgt ggcgacggta cggatcagca gggcctcgtg
     8461 cacctgccgc ggcgtgaagt agcggctcca gaggtagcct tcggtggggg ccaacaggtg
     8521 tcgttgccgg aggctgcccc ggctgccgcc gcgcgtcatc acgcgcgccc ggtgctgccg
     8581 caggccgccg cgcggcgcgt gcaggtgcac gaccgacgcc gccgggttca gccccagcgt
     8641 cttgccggcc aggtagaggc gcatacccag gtcgccgtcg gcgcgctcgc cgcgctcata
     8701 ggccaggtcg aacaggcccg cgtcggccag cgccgccgtg cgcagcaggg cgttattggt
     8761 cggaaagacg tcgctctcgc gcacgcgccg gaacgcggcc ggcagcgccc ccgcgcccaa
     8821 ctcctcggcc acgccgcagg aggcatcgac gccgaaacgg gccagatagg ccaggtggcg
     8881 ggcgatcagg tcgggcggcg cttcgtcgtc gtcgtccagg aagagcacgg cgtcgccgcg
     8941 cacaatggcc agcccggcgt tgcgcgagga gcattgcccg gctgtgtcgc gccggatgac
     9001 ccgcaggggc aggtcggcga agcgcgccgg ccacgccagg tcacgcgccg cggcctcggt
     9061 ctggtcaacg acgatgatct ccagcggcga cacggtctgc gcccgcagat ggtcgagcag
     9121 gacgaacaga tgcgggtagc ggtccagcgt ggggatgagc acagagaccg tcggggcgtc
     9181 cgcgccaacc gcggtgggca actcgacggg gtgcagcgtg ccggccgggc cagcgcctgc
     9241 cgccggccgc gccgcgcgga aggcccgcgc tgtggccagc agccctgcgc catgtcgcgc
     9301 cgcccgccag gccgcccacg ccgcccacat gcggccatag cgccggcgca cgaaacggaa
     9361 ttcgtcggct ggtgtcggcg cgtcgaccgc gccggtcggc gcggccacca ggtcgggcac
     9421 gtagcgcagc atcgccccgc ccgtcaccca gcggtggccc agctccagcg ccgccccggc
     9481 cagcgtgtcg aagccggccg ccgggccgcc caggcagcgc accacgtcgg cgcgcatcag
     9541 gcaggcccgc agcgacagcc gccaggagac gcccggcgcg gcggggttcg ggtcgcggtt
     9601 gaagcgccag acggggtcaa catagtcgat cagcgccggc aagccggcca tgcccagccg
     9661 cagcccggcg tgccagacgt cgcccggccc ggccagtaga gcggcgacga gatcggcgtc
     9721 cggctggccc agcgccgcgt cccagaagag gagggccgcg ggggagcggg gggacggggg
     9781 agcagggggg gcaagagcgg ccgccgccga cactatggcg gcgaccgtgg cgggcacaag
     9841 gcgcgtctcg cctagcggcc aggcgggggc cggccnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnngcggc gatctgcgcc tccggccgga agcaagtctg cacccgctcg
    10021 cgcccggccg cgcccatctg cgcgcgcaac tccgtgtcgg ccgccagccg ggccagcgcc
    10081 gtcgccaacg ctgccggctc gcgccgcggc acgacgaagc cagagacgcc atcggcgacg
    10141 ttctcggcca ggccgtcggc gtcgctgcac acgaccggca gggccatcgc ctgcgcctcc
    10201 aggacggcgt tgccgaagcc ctcggacacg gccgggtgca gcagcacgtc ggcccactgc
    10261 atctgcgccg gcacgtcggc cggcggacgc gcccccagga actccacctc ggccgccagc
    10321 cccatctggt ggcgggcgaa ggccagcgcc tccagatatt gcccgccgcc gaggatgcgg
    10381 tagtggacag gcacgccgcg cgccttcagt agccggacgg cctccaggcc aacctcgtag
    10441 cccttcttcc actccaggcg gccgacgctg aggacgcgca acggccggcc cgggccaatg
    10501 gcgtcatcgt cgcgcgccgc cggccggaag acctcggcat cgacggccgg cgggatgagc
    10561 gcgtggggca tgtcggccgg gcagccgcgc cgccgcgccc gccgccacag atcgccgccg
    10621 agcgtgtgga tggcgtcggc gtcgcgccag atggcggcgt agtggccggg atcgtccagg
    10681 ccgacgtagt tcaggtcata gccgcggaag ctgaccgtca ggcggcaacc cagccgctgc
    10741 tttagcgccg tccggcccac ggccagcgcg ccgaactcga aatgcaggat gtcgggcgac
    10801 agggcgatga tggccgcgtc caggtagaag tcgcgcgccg cccgccgcag cccgcccggc
    10861 agcgcccgcc gccagtagcg cagcgtcgcc cgcggcgcgc gcagcagcgt cagcagcagc
    10921 gccggcagcc acagcagcgc cacctgccag gccgcggcgt ggggccactg gcgatggacg
    10981 cgccggcgca agtgtggccg ggccagcagg gccgggaagg ccgtccacgc ctcggcctcc
    11041 gcgcaaacga cgtggacatc ccagccggcg tcggccaggc cgacgaactt gctgacaatg
    11101 aacgtctccg acagccgcgg aaaccggggc acgaccagca ccagtctcat gccgccaccg
    11161 cccggaagag gtcagcgaag gccccgacct gatcgctcag ccggaaatcg cgctcgacgc
//
LOCUS       DAOSMQ010000032        11159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5822_length_11159_cov_5.308537, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000032 DAOSMQ010000000
VERSION     DAOSMQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5822_length_11159_cov_5.308537"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2136)
                     /gene="phnE"
                     /locus_tag="PK829_01970"
     CDS             complement(<1..2136)
                     /gene="phnE"
                     /locus_tag="PK829_01970"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOU40011.1"
                     /translation="MPPERHSANPWLPALLSLIIPGSGQFLLRERSRGITLFLTFLAL
                     LGLVLWTRTYALLAPLALLLLWIARDAYQLARGKQPRWGAALLLIGLVLYGAATFITE
                     VRPTRLITGLPNVQPYLRSLLNPELFEYPTEDLAGVAPIMVPCVDPLPEPNREATVSP
                     RIITLPCTEVGDVLAISGEGFQPNAAGQVQWVDPLGSPRRATLNGQVVTFVADDDGRF
                     TATLVVPQAVPLTAQPDPGQTLTHAVRAVQAIPTGGLQPTQTLSLVIEKIGETLALAF
                     LSTVMGVLFAVPVSFLAARNLMGANRFTLAVYYVVRTLLNIIRSIETLMWAIVFAVWV
                     GLGPFAGTLALWFHTVAALAKLYSESIESIDPGPIEAVRATGASWAQMVVYAVLPQIL
                     PTFTSFTLYRFDINVRLSTVIGLVSDAGLGFLVIQWVRLNRFSAMATAILAIILVVAT
                     LDFVSGWLRERIIAGRPIISSRNPAVRSALRLLIVAGFVAAFAWSWRVAQIEPVELIR
                     GAPKGLALAREFAVPEVFTQPTTTQAISAPLPVPCDAVDPGEPADEGISLSVACGDPG
                     EPLIITGSGLPPNRQVSVRWVLPDGAFLRVRAGCCTTTPEGTLTVETTINPVVAFQEG
                     QPAASRVEITYEDVAGRRQLSDTVRTVFNLSILTLLMALVATTLGALFAIPLSFLAAR
                     NIMGNSPLGKTIYYGLRTIFNVTRSVEPLI"
     gene            complement(2126..2899)
                     /gene="phnC"
                     /locus_tag="PK829_01975"
     CDS             complement(2126..2899)
                     /gene="phnC"
                     /locus_tag="PK829_01975"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOU40012.1"
                     /translation="MLEIQNLTKVFPDGTVALKEVSFRVEDGEFLAIIGLSGSGKSTL
                     LRCINRLIEPTAGAVIWNGQNITAANTSELREIRRQIAMVFQHFNLVKRSSVTTNVLT
                     GRLGYVNPFWSLLGRFSAEDRQRARAALARVGIEDKADNRADQLSGGQQQRVGIARAL
                     MQEPKLLLADEPVASLDPVLSHSILRYLELLNQQDDITVLCSLHFLDLVHRYATRVVG
                     LKAGELVFDGLPSELTPAKFKEVYGEEAEMVSVTPQNAS"
     gene            complement(2989..3981)
                     /locus_tag="PK829_01980"
     CDS             complement(2989..3981)
                     /locus_tag="PK829_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU40013.1"
                     /translation="MNVRKFSLLLVVAAFLMLALAACGGGAAEVTPTEAPAVEEPTTA
                     AEEPTAAVAEPAAGDLGTAENPIIMSFVPSGDTQDIIASGDTLAQMLTDATGLTVQST
                     VGTDFSSVREAMCAGQAQIGWLNTFNYVMAAEQCGVDAALVTSRFGATTYAGQIIVRA
                     DSGIKTLQDLKGKVMCWVDPASTSGYIIPRIMLAAEGIDPDTDFAETIEAGSHNNVVT
                     QVYNGDCDAGATFSDARTGIEEEFPDVLEKVVVLANTSDIPNDSVAFIADFPAAMREQ
                     IVTALLDIAGTPEGQEALNTLYNIEGLQASSDAFYDTFRADLSRAGLNIEELAQ"
     gene            complement(4129..4749)
                     /locus_tag="PK829_01985"
     CDS             complement(4129..4749)
                     /locus_tag="PK829_01985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU40014.1"
                     /translation="MTTTISLLRHGLVENPDAVYYGRLPHFGLAERGRAQATAAGRYL
                     AGTEVSALYHSPMLRARQTAELVAAQLAAPVPLLPCDLLTEIHSPYDGETIAVMEQRH
                     WDFYGEIEPPYETPADILARILAFFAHARAERPGQHVVGVSHGDPIAFAILWAFGRPA
                     TSAAKRELSECGVDDLYPAPASISTFRFDEAGALVEYGYYCPHDDK"
     gene            complement(4746..5441)
                     /locus_tag="PK829_01990"
     CDS             complement(4746..5441)
                     /locus_tag="PK829_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU40015.1"
                     /translation="MAETILIADDEASIRDLARLYLEREGFRVRMAVDGAAALDEVRR
                     DPPALLILDLMMPRMDGWEVCRRVRAESNLPILMLTARDQAIDKIVGLEMGADDYLTK
                     PFEPRELVARVRAILRRTTVDKPASDRPRHLGRVTIDPGSREVLLDETPIFLRAREFD
                     LLLMLADHTNQVLSRDQLLDLVWGYEYHGQTRTVDVHVAHLRDRLEGSGVEIETVWGR
                     GYKLSVVEAGPAT"
     gene            complement(5449..5835)
                     /locus_tag="PK829_01995"
     CDS             complement(5449..5835)
                     /locus_tag="PK829_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU40016.1"
                     /translation="MNKQRIHTDDAPAAVGPYSQAIRAGNFLFTAGQVALDPAAGDLV
                     GSDVAAQTEQVLRNLRAVLAAAGADFAHVVKTTVFLQSMSDFAAMNAVYARHFPEPFP
                     ARSTVEVGALPKGGLVEIECVALLAD"
     gene            complement(5920..6504)
                     /locus_tag="PK829_02000"
     CDS             complement(5920..6504)
                     /locus_tag="PK829_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40017.1"
                     /translation="MKLLLLRCPKCNQALSPGQDDRVIQCPNCHEAVSLDEGGLRLLP
                     VQYVTPIQPRPEAWLPFWVYRGEVTIQVRETQGGRSAEDEARAFWAKTRFVYIPAWSC
                     ELAEARALATDLLQKQPLLMAVSPPEGATFPPVALAPEDGRKLVELVIVSIEAGRRDW
                     MERLKYDLRLDSEALWLLPARRDGDGWALLIKGL"
     gene            complement(6508..7641)
                     /locus_tag="PK829_02005"
     CDS             complement(6508..7641)
                     /locus_tag="PK829_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40018.1"
                     /translation="MTTEPTPASPAPVSTGPRGRVPERSQGLVCPNCSGTVPVAEGAR
                     IVQCPYCNLHSLVQGERGVRRWQVPRQVDRARAEAGVRGFLSGMRKARDLSRAASIDE
                     LMLTYLPFWRVEATVAGWLFGRVRKDKDETKPDEHEVFELMNWNDAAVDVSEFGVHHI
                     VVSRAELQPFESQNIHAEAMVFEPTESRTDALDEARGYFLGRARSAASQRSTSYENVQ
                     LLRPEFALVYYPVWIGRYSYRSRTYQVVIDGVSGRVMYGKAPGNVLYRAMALVVGLAA
                     GNLVLVNGTMLAARASSDDNSLGLLLLPIVVGIGLILNGYRQFRYGEEVEDRPKEFQK
                     AGSGGGLLGSFLPTTGNLSEMMKTGQSVLVDLEKISREARRDR"
     gene            complement(7659..8471)
                     /locus_tag="PK829_02010"
     CDS             complement(7659..8471)
                     /locus_tag="PK829_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40019.1"
                     /translation="MSDAYVSTQLLCQQCSAPLSVELGAKFVTCEFCNTVNFVDKSQT
                     VLHYTVTPTLDEPGAAAALRRWMAGNQTVKGLDQAAQIESQTYQLFPLWQVRTVRDDK
                     EKVVFKPAAALSIVELGKLSVPASDLAPYDDTLDAVAVKPTVPVTAVHSWLASNEGIA
                     AGTIEELSLVHVPIYQFKYRYKDQSYTALVDAAAGEVFTAIYPEKFELPYVSIGGFGC
                     LAYFLAALIPIITLAVTNSDIGLLLGILIYLGVAVVLAIPIFMVAASISRRF"
     gene            8635..9192
                     /gene="efp"
                     /locus_tag="PK829_02015"
     CDS             8635..9192
                     /gene="efp"
                     /locus_tag="PK829_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOU40020.1"
                     /translation="MIDVNQLRRGVTFVHDGALLKVNEYSHSKPGRGKATIRVRVRDL
                     RTGTNREVTFSSGDRVEDVRLDKSTYQYLYDDGSFYVFMDQATYEQRQVGHSVLEDDK
                     AFLRDNMELELLSYEGEVIDYELPKTMEFDVVEAENALAGDTATGATKEVVTETGLRV
                     KTPLFVQVGDRIRVNTETREYLTRV"
     gene            9263..9724
                     /locus_tag="PK829_02020"
     CDS             9263..9724
                     /locus_tag="PK829_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU40021.1"
                     /translation="MSREKSVGRLVFLAALVVLLSLFFVAARPAEAQGAGVNHIVQPG
                     EFLALIARNYGTTVQNILWANPQITNPNRIYPGQVIFVPLGVVTPPPPLPPPTPQPLP
                     QPICRAWHYVTPGQTLLQISRWYGVDPFAIARANGIFNLNLIYWGTNLCIP"
     gene            complement(9802..>11159)
                     /locus_tag="PK829_02025"
     CDS             complement(9802..>11159)
                     /locus_tag="PK829_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU40022.1"
                     /translation="APADGDTPVGQAAPFEDAGTVIGSGPYSGLPSAEAAARIAADLA
                     TRGLGGATVSYRLHDWLISRQRYWGAPIPIVYCDACGIVPVPEAQLPVRLPDDLPRPT
                     GDGRSPLAGIETWVNTTCPQCGGPARRETDTMDGFACSSWYFLRYASPHETARPFDPA
                     AVAAWLPVDVYVGGAEHAVMHLLYARFWTKVFHDAGLIDFDEPFTQLRNQGMLLSPQD
                     GQKMSKSRGNVVTPDEVVAAHGADALRAYVLFLGPFDANALWDDRGIVGVDRFLERYW
                     RLAHEFATLPDGPFNVAFERARHVVISRVTADMERFRFNTAVAALMEYTNTLFAARQQ
                     PVAAAQWRTAIRSLTLLLAPIAPFITEEVWREVLGELESVHRAAWPAWDEAMTVEEQV
                     TVVVQVNGKLRDRLTAAADSDAAALRAMALANDRVRAAIDGRAVRDVIVVGRLVNVVT
                     T"
BASE COUNT         1955 a         3531 c         3843 g         1830 t
ORIGIN      
        1 gatgagcggc tcgaccgagc gggtgacgtt gaagatcgtc cgcaggccat agtagatcgt
       61 cttgcccagc gggctgttgc ccatgatgtt gcgcgccgcc agaaagctga gcgggatggc
      121 gaacagcgcg cccagcgtcg tggccacgag ggccatgagc agggtcagga tggacaggtt
      181 gaagacagtg cgcacggtgt cgctgagttg ccggcggccg gccacgtcct cataggtgat
      241 ctccacacga ctggcggccg gctgtccctc ctggaaggcg acgacgggat tgatcgtcgt
      301 ctccaccgtc agcgtgcctt ccggcgtcgt tgtgcagcag ccggcgcgga cgcgcaggaa
      361 ggctccgtcg ggcagcaccc agcgcaccga cacctgccgg ttgggcggca ggccgctacc
      421 ggtgatgatc agcggttcgc cggggtcgcc gcacgcgact gagagactga tgccctcgtc
      481 ggccggctcg ccggggtcta ccgcgtcgca gggcacaggc aggggcgcgc tgatggcctg
      541 ggtcgtcgtc ggctgggtga agacttccgg cacggcaaac tcgcgggcta acgccagccc
      601 ttttggcgcg ccacggatca actccaccgg ctcgatctgg gcgacgcgcc acgaccaggc
      661 gaaggcggcc acaaagccgg cgacgatgag cagacgcagg gcgctgcgca cagccgggtt
      721 ccggctgctg atgatcggcc ggccggcgat gatgcgttcg cgcagccagc cggacacaaa
      781 gtccagcgtg gccacgacga ggatgatggc gaggatggcc gtggccatgg ccgagaagcg
      841 attgagccgc acccactgga tgaccagaaa gcccaggccg gcgtcgctga ccagaccgat
      901 gacggtcgag aggcgcacgt tgatgtcgaa gcggtagagg gtgaacgacg taaacgtcgg
      961 cagaatctgg ggcagcacgg cgtagacgac catctgcgcc caactggccc ccgtggcccg
     1021 cacggcctca atggggccgg gatcgatgct ctcgatggat tcagagtaga gcttggccag
     1081 ggcggcgacg gtgtggaacc acagcgccag cgtgcccgcg aacggcccca gaccgaccca
     1141 gacggcgaag acgatggccc acatcagcgt ctcgatggag cggatgatgt tgagcagcgt
     1201 gcggacgacg tagtagacgg ccagggtgaa gcggttagcg cccattagat tgcgtgccgc
     1261 caggaagctg accggcacgg cgaagaggac gcccatcacc gtggacagga aggccagggc
     1321 cagcgtctcg ccgatcttct cgatgaccag cgatagtgtc tgcgtcggct gtagcccgcc
     1381 ggtagggatg gcctgcaccg cgcggacagc gtgggtcaat gtctgaccgg ggtcgggttg
     1441 ggccgtcagg ggcacggcct ggggcacgac cagtgtggct gtgaagcggc cgtcgtcgtc
     1501 ggcaacaaag gtcacgactt ggccgttgag cgttgcccgc cgagggctgc ccagcggatc
     1561 gacccactga acctgcccgg cggcgttcgg ctggaagccc tcgccgctga tcgccagcac
     1621 gtcgcccacc tcggtgcagg gcagggtgat aatgcgcggc gagacagtcg cttcgcggtt
     1681 tggctctggc agcggatcga cgcagggcac catgatcggg gcgacgccgg ccaggtcttc
     1741 ggtcgggtac tcaaatagct ctgggttgag cagcgagcgc aggtagggtt ggacgttggg
     1801 caggccggtg atcagccgcg tcggccgcac ttcagtgatg aacgtggccg cgccatagag
     1861 taccaggccg atgagcagca gggcggcgcc ccaacgcggc tgcttgcccc gcgcgagctg
     1921 gtaggcgtcg cgggcgatcc agaggagcaa cagcgccagg ggggccagca gggcgtaggt
     1981 tcttgtccac agcaccagac ccaacagggc caggaaggtc aggaagaggg tgatgccccg
     2041 tgagcgttcg cgcaggagga attggccgct gcccgggatg atgagcgaga gcagggcggg
     2101 caaccaggga ttggcggagt gtcgttcagg aggcattctg cggcgttacc gacaccattt
     2161 ccgcctcttc gccatagact tctttgaact tcgccggcgt cagctccgat ggcaggccat
     2221 cgaagacgag ttcgcccgct ttcaggccca cgacgcgcgt ggcatagcga tgtacgaggt
     2281 ccagaaagtg caagctgcac aggacggtga tgtcgtcttg ttgattgaga agttccagat
     2341 agcgcaggat ggagtgcgac agcaccgggt cgaggctggc caccggctcg tcggccaaca
     2401 gcagcttcgg ctcctgcatc agggcgcggg caatgccgac gcgctgctgc tggccaccgg
     2461 agagctggtc ggcgcggttg tcggccttgt cctcaatgcc cacgcgggcc agcgccgccc
     2521 gcgcgcgctg gcgatcctcg gccgagaagc ggccgagcag gctccagaag ggattgacgt
     2581 agcccagccg cccggtcagc acgttggtgg tcacgctgct gcgcttgacc aggttaaagt
     2641 gctggaagac catggcgatc tgccggcgaa tctcgcgcag ttccgacgtg ttggcggcgg
     2701 tgatattctg gccgttccag ataaccgcgc cggccgtcgg ctcgatcagc cggttgatgc
     2761 agcgtagcag cgtcgatttg cccgagccgg acaggccgat gatggccagg aactcgccgt
     2821 cctcgacgcg aaagctgacc tccttcaggg ccaccgttcc atcggggaag accttggtca
     2881 ggttctgaat ctcgagcacg gcttctcctt cgttcgcgaa gcggccacga agcggccacg
     2941 gaccagcgtc ccggtccgtg gccgcttggg tcagctacac agcagtggct actgggccaa
     3001 ctcctcgatg ttgaggcccg cgcggctcag gtcagcgcgg aaggtgtcat agaaggcgtc
     3061 gctggacgct tgtagcccct caatgttgta gagggtgttc agcgcttcct gcccctccgg
     3121 cgtgccggcg atgtcgagca gggcggtgac gatctgctcg cgcatagccg cggggaagtc
     3181 ggcaatgaag gccacgctgt cgttggggat gtcgctggtg ttggccagga cgacaacctt
     3241 ctccagcaca tccgggaact cctcttcgat gcccgtgcgg gcgtcggaga aggtcgcgcc
     3301 ggcgtcgcag tcgccgttgt agacctgggt gacgacgttg ttgtgcgaac ccgcctcgat
     3361 ggtctcggcg aagtcggtgt cggggtcgat gccctcggcg gccagcatga tgcgcgggat
     3421 gatgtagccg gaggtcgagg ccgggtcaac ccagcacatg accttgccct tcaggtcttg
     3481 cagcgtcttg atgccactat cggcgcggac gatgatctgg ccggcgtagg tcgtcgcccc
     3541 aaagcgggac gtcaccaggg cggcatcgac gccgcactgc tcggcggcca tgacgtagtt
     3601 gaacgtgttc agccagccga tctgggcctg accggcgcac atggcctcgc gcacagacga
     3661 gaagtccgtg ccgacggtgg actggaccgt caggccggtg gcgtcagtga gcatctgcgc
     3721 cagggtgtcg ccgctggcga tgatgtcctg ggtgtcgccc gaggggacga aggacatgat
     3781 gatgggattc tcggccgtgc ccagatcgcc ggcggccggc tctgcgacgg ccgcagtcgg
     3841 ctcctcagcc gcggtggttg gctcctcgac agcgggcgct tccgtcggcg tgacttcggc
     3901 ggcgccaccg ccacaggcgg ccagggcaag catcaggaat gctgccacta ccaatagaag
     3961 ggaaaactta cggacgttca ttctttcctc caggactaag acgagccaca attagctcgc
     4021 gcttgacggc ggcgacgcct tcataggacg gctcgccgcc gttgaacctg ctgtcatgtt
     4081 acataccttg ccaatggttg acaaccgtaa tatgacccat cggcggcctt acttatcgtc
     4141 gtgcgggcag tagtagccgt actctaccag cgcgccggcc tcgtcgaagc ggaaggtaga
     4201 gatggacgcc ggcgcggggt agaggtcatc gacgccacat tcgctcagtt cgcgcttggc
     4261 cgcgctcgtg gccggccggc cgaaggccca caggatggcg aaggcgatcg gatcgccgtg
     4321 gctgacgccg acgacgtgtt ggccggggcg ctcggcccgc gcgtgggcaa agaaggccag
     4381 tatccgtgcc aggatgtcgg ccggcgtctc gtagggcggt tctatctcgc cgtagaagtc
     4441 ccagtggcgt tgctccatca cggcgatagt ctcgccatcg tagggggagt gaatctcggt
     4501 cagcaggtca caggggagca gcggcacggg cgcggccagt tgcgcggcca ccagttcggc
     4561 cgtctggcgg gcgcgcagca tggggctgtg gtagagggcg ctgacctccg tgccggccag
     4621 gtagcgcccg gcggctgtgg cctgggcgcg ccctcgctcg gccaggccaa agtggggcag
     4681 gcggccatag tagaccgcgt ccgggttctc taccagaccg tggcgcagca gcgaaatggt
     4741 cgtggtcatg tcgccgggcc ggcctccacg acgctcagct tgtagccccg cccccaaacc
     4801 gtctcgatct ccacgccgct gccctccagc cggtcgcgca gatgggccac gtggacatca
     4861 acagtgcgcg tctggccgtg gtactcgtag ccccagacga ggtcgagcaa ttggtcgcgc
     4921 gacagcacct ggttggtgtg gtcggccagc atcagtagca ggtcgaactc tctggcacgc
     4981 aggaagatgg gcgtctcatc gagcagcacc tcgcggctgc cggggtcgat ggtgacgcgg
     5041 ccgaggtggc gcggccgatc ggaggccggc ttatcgaccg tcgtgcgccg caggatggcg
     5101 cgcacgcggg ccaccagctc gcgtggctca aatggcttgg tcagatagtc gtcggcgccc
     5161 atctccaggc cgacgatctt gtcgatggcc tgatcgcgcg cggtcagcat caagatgggc
     5221 aaattgctct cagcgcggac acgacggcag acctcccagc cgtccatgcg cggcatcatc
     5281 agatcaagga tgagcagtgc cggcggatcg cgccgcacct catccagcgc cgccgcgccg
     5341 tcgacggcca tgcgcacgcg aaagccctcc ctttccaggt agagccgggc caggtcgcgg
     5401 atgctggcct cgtcgtcggc aatcaggatc gtctcggcca tcgcgcactc agtcggcaag
     5461 gagtgccacg cactcgatct cgaccaggcc gcccttgggc agcgcgccta cctcgaccgt
     5521 ggaacgggcg gggaacggct ccgggaagtg gcgagcgtag acggcattca tggccgcgaa
     5581 gtcgctcatg ctctgtagga agacggttgt tttgaccacg tgggcgaagt cggcacccgc
     5641 ggcggccagc acggcgcgca ggttgcgtag cacctgctcg gtctgcgcgg cgacgtcgct
     5701 gcccaccagg tcgcccgcgg ccgggtcgag ggcgacctgc ccggccgtga acaggaagtt
     5761 accggcccgg atggcctgcg aataggggcc gactgccgcc ggagcgtcgt cggtgtgaat
     5821 gcgctgcttg ttcatggcta ctccttgtgc ccgctcagcc taagaataag cgggcagtgt
     5881 ttagaaagtg tgaaggatgt atgatgtggg tgtaacggct tagaggccct tgatcagcaa
     5941 cgcccagccg tcgccgtcgc gccgcgctgg cagcagccag agcgcctcgc tgtcgagccg
     6001 caggtcatac ttgaggcgct ccatccagtc gcgccgcccg gcctcaatgc tgacaatgac
     6061 cagttccacc agcttgcggc cgtcctctgg ggcgagggcg acgggcggaa acgtggcccc
     6121 ctccggcggc gagacggcca tcagcagcgg ctgcttctgc agcaggtcag tggccagcgc
     6181 gcgcgcctcg gccagttcgc acgaccaggc agggatatag acgaagcgcg tcttagccca
     6241 gaaggcgcgc gcctcgtcct cggccgagcg gccgccctgc gtctcgcgca cctggatggt
     6301 cacctcgccg cgatagaccc agaacggcag ccacgcttcc ggccgcggct ggatgggcgt
     6361 gacgtactgc accggcagca gccgcaggcc gccctcgtcc aggctgacgg cctcgtggca
     6421 gttggggcac tggatgaccc tgtcatcctg gccgggggac agggcctgat tgcacttggg
     6481 acagcgaagc agtaaaagtt tcatggctca tcgatcgcgg cgcgcctcgc ggctgatctt
     6541 ctccaggtca accaggacgc tctggccggt cttcatcatc tccgagaggt tgcccgttgt
     6601 tggcaggaac gaacccagca ggccgccccc gctgcccgcc ttctggaact ctttgggccg
     6661 atcctcaacc tcctcgccgt agcggaactg gcggtagccg ttgaggatca gaccgatgcc
     6721 gacgacgata ggcaacagca gcaggcccag gctattgtca tcggacgagg cgcgggcggc
     6781 cagcattgtg ccgttgacca ggacgagatt gccggcggcc agcccgacga ccagggccat
     6841 ggcccggtag aggacgttgc caggcgcttt gccatacatc acccgcccgc tgacgccgtc
     6901 gatgacgacc tggtaggtgc ggctgcggta ggaatagcgg ccgatccaga ccgggtagta
     6961 aaccagcgcg aactccggcc gcaacagttg tacattctca tagctggttg accgctggct
     7021 ggcggcgctg cgcgcccggc ccaggaagta gccgcgcgcc tcgtccagcg cgtcggtgcg
     7081 cgattccgtc ggctcgaaga ccatcgcctc ggcgtggatg ttctggctct cgaacggctg
     7141 caattcggcc cgcgagacga cgatgtggtg aacgccgaac tcggagacat cgacggcggc
     7201 gtcgttccag ttcatcagct cgaagacctc gtgctcgtcg ggcttggtct cgtccttatc
     7261 cttgcgcacg cggccgaaga gccagccggc cacggtagcc tcgacgcgcc agaagggcag
     7321 ataggtcagc atcagttcgt caatcgacgc cgcgcggctc aggtcgcggg ctttgcgcat
     7381 gcccgacagg aagccgcgca cgccggcctc ggcgcgcgcc cggtcgacct ggcgcggcac
     7441 ttgccagcgg cgcacgccgc gctcgccctg caccagcgag tggaggttac agtaggggca
     7501 ctgaacgatg cgcgcgcctt cggccacagg caccgtgccg ctgcaattgg ggcagacaag
     7561 gccctgcgag cgctccggaa cacggccgcg cggcccggtg gaaaccggcg caggggaagc
     7621 aggagtgggt tcagtcgtca tgggcagtcc tcaagcgctc aaaagcgacg cgaaatcgaa
     7681 gcggcgacca tgaagatggg aatggccaga acgacggcca cgccgagata gatgagaatg
     7741 cccagcaaca ggccaatgtc gctgttggtc acggccaggg tgataatggg aatgagcgcg
     7801 gccaggaagt aggccaggca accgaagccg ccaatggaga cgtagggcaa ctcgaacttc
     7861 tccgggtaga tggcggtgaa aacctccccg gcggcagcat caaccagggc ggtgtaggac
     7921 tgatccttgt agcggtactt gaactggtag atgggcacgt gaacaagcga caactcctca
     7981 atcgtgccgg cggcgatgcc ctcattgctg gccagccagc tatgcacggc cgtcaccggc
     8041 acggtcggct tcacagccac ggcatcgagg gtgtcgtcat agggagccag gtctgacgcc
     8101 ggcacgctca gcttgcccaa ttcgacgatc gatagcgccg cggcgggctt gaagacgacc
     8161 ttctccttgt cgtcgcggac ggtgcgcacc tgccagagag gaaatagctg gtaggtctgc
     8221 gactcgattt gcgcggcctg atccagtccc ttgacggtct gattgccggc catccagcgg
     8281 cgcagagcag ccgcggcccc tggttcgtcc agcgtcggcg tgacggtgta gtgcaacacg
     8341 gtctggctct tatcgacaaa gttaaccgtg ttgcagaact cgcaggtgac gaacttggcc
     8401 cccaactcca ccgacaacgg ggccgagcat tgctggcaca gtaactgggt actgacatac
     8461 gcatcagaca taccggcgca cctcagcgtc attgtagcac gcgcatggcc aaaggaaagt
     8521 gccgtcgctt tccgctgccg cgcgcgcctg atccgttgca cggtaaggcg aacctatggt
     8581 acactgtgag gtaattgaca ttatgtcttg tgcgttagga gtagaattgc agcgatgatc
     8641 gacgtcaatc agctgcgccg tggggtgaca tttgtccacg acggcgcgct tctgaaagta
     8701 aacgagtatt cccacagcaa gcctggccgc ggcaaggcga caatccgcgt ccgcgtccgc
     8761 gacttgcgca cgggcacaaa tcgcgaggtc acctttagct ccggcgaccg cgtggaagat
     8821 gtccgtctcg acaagagcac ttaccagtac ctctacgacg acggtagctt ctatgtcttc
     8881 atggatcagg ccacctacga gcagcggcag gtcggccaca gcgtgctgga agacgacaag
     8941 gctttcctgc gcgacaacat ggagctggag cttctgtcct atgaaggcga agtcatcgac
     9001 tacgagcttc ccaagacaat ggagttcgac gtggtcgagg cggagaatgc cctggcgggc
     9061 gacacagcca cgggcgcgac caaagaggtt gtcacggaga cgggcctgcg ggtgaagacg
     9121 ccgctgtttg tccaggtggg cgaccggatt cgcgtcaata ccgagacgcg tgaatatctg
     9181 acccgcgtct agcacccatg cgcgacttcc cggtgggctt ttctctaatc ggtttagtct
     9241 ggagggtaca atgagttggg tagtgtctag agagaagtcg gtcggccgtc tggtcttcct
     9301 ggcggccctg gtggtgctcc tgtctctgtt cttcgtggcc gcgcggccgg cggaggcgca
     9361 gggtgcgggc gtaaaccaca ttgtccaacc gggcgagttt ctggcgttga tcgcccgtaa
     9421 ttatggcacc acggttcaga atatcctgtg ggccaatccg cagatcacca atccgaaccg
     9481 catctatccg gggcaggtga tcttcgtccc gcttggcgtg gtgacgccgc cgccgcctct
     9541 gcccccaccg acgccccagc cactgccaca accgatctgc cgcgcgtggc actatgtgac
     9601 gccgggccag acgctgctgc aaatcagccg ctggtatggc gttgacccgt tcgccatcgc
     9661 ccgagccaat ggcatcttca acctgaatct gatctactgg ggcacgaatc tgtgtatccc
     9721 ctagcggttt gtgggggaag ggccttgtag cccagccccc acagccggcg cgtcatcatc
     9781 gggcgggtgc gaacccgccc tttatgttgt cactacattc accagccgcc cgacgacgat
     9841 gacatcgcgc accgcgcggc cgtcaatggc cgcccgcacg cggtcgttgg ccagagccat
     9901 ggcccgcaga gcggcggcgt cgctgtcggc cgcggccgtc agccggtcgc gcagcttgcc
     9961 attgacctgg acgacgaccg tcacctgctc ctcgacggtc atggcctcat cccaggccgg
    10021 ccacgctgcc cgatgaaccg actccagctc gcccaacacc tcgcgccaga cctcctcggt
    10081 aatgaacggg gcgatgggag ccagcagcag tgtcagtgag cggatggccg tgcgccactg
    10141 cgcggcggcg accggctgct ggcgcgcggc gaacagcgtg ttggtatact ccatcagcgc
    10201 ggccacggcg gtattgaagc ggaagcgctc catgtcggcc gtgacgcggc tgatgacgac
    10261 atggcgggcg cgctcgaagg cgacgttgaa cgggccgtcg ggcagcgtgg cgaactcgtg
    10321 ggccagccgc cagtaacgct ccaggaagcg atcgacgccg acgatgccgc ggtcgtccca
    10381 cagggcgttg gcgtcgaacg gccccaggaa cagcacgtag gcccgcagcg cgtcggcccc
    10441 gtgggcggcc acgacctcgt cgggcgtgac gacgttgccg cgcgacttgg acatcttctg
    10501 cccatcctgc ggcgacagca gcatgccctg gttgcgcaac tgcgtgaatg gctcgtcgaa
    10561 gtcgatcagc ccggcgtcat ggaagacctt tgtccagaag cgggcataca gcaggtgcat
    10621 gacggcatgc tccgcgccgc cgacgtagac atcgaccggc agccaggcgg ccacggcggc
    10681 ggggtcgaac ggccgcgccg tctcgtgggg gctggcgtag cgcaggaagt accacgacga
    10741 gcaagcaaag ccgtccattg tgtcggtctc gcgccgggcg gggccgccgc actgcgggca
    10801 ggtcgtgttg acccacgtct cgatgccggc cagcggcgag cggccgtcgc ccgtcggccg
    10861 tggcaggtcg tcgggcagcc gcacgggcag ctgtgcctcg ggcacgggca cgatgccgca
    10921 ggcgtcgcag tagacaatgg gaataggcgc gccccagtag cgctggcggg agatcagcca
    10981 atcgtgcagg cgatagctga ccgtggcccc gcccaacccg cgggtggcca ggtcggcggc
    11041 gatgcgcgcc gccgcttcgg ccgagggcag gccgctgtag gggccggagc cgatgaccgt
    11101 gccggcgtcc tcgaagggcg cggcttggcc gaccggggtg tcaccgtctg ccggcgcaa
//
LOCUS       DAOSMQ010000033        11101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5856_length_11101_cov_5.381948, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000033 DAOSMQ010000000
VERSION     DAOSMQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_11101_cov_5.381948"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK829_02030"
     CDS             complement(<1..405)
                     /locus_tag="PK829_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU40023.1"
                     /translation="MTSQSHPPERAIAPQQQADDVGLDTMLRPQRLTQFTGQDRLKDN
                     LRILIEAARARGETLDHVLFHGPPGLGKTTLAQVVANEMNANIRHTAGPAIEHSGDLA
                     AILSNMRTGDILFIDEVHRLSRPVEEILYPAME"
     gene            667..1287
                     /gene="upp"
                     /locus_tag="PK829_02035"
     CDS             667..1287
                     /gene="upp"
                     /locus_tag="PK829_02035"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOU40024.1"
                     /translation="MVFESHHPLVKHKLTLMRDSTTKPKKFRELIREISLLLCYEATT
                     DLTTQPRTVDTPMGRADGVELLHKVGLVPVLRAGLGMVEGIWEMMPGAEVWHIGLYRD
                     ERTLKPVSYYNKLPTMPTVQVCLVLDPMLATGGSAVATVDILKRWGAERIKFVGILAA
                     PEGIARLQGAHPDVPIHVAHVDERLNDIGYIVPGLGDAGDRQFGTG"
     gene            1223..2338
                     /locus_tag="PK829_02040"
     CDS             1223..2338
                     /locus_tag="PK829_02040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOU40025.1"
                     /translation="MTSATLSLAWATPATGSSAQGNVVAFVVTLAALVVAAATTPLAR
                     RLSFRWGIVAEPGGRRQHHGRMPKLGGLAVAAGWAVGVALIYLLRPPGNPDDALRLRG
                     VVLGSLIVLAGGLLDDRYDLPPWAQLVIQLSGALVAIAHIIFIEVFTNPLPGTALWQA
                     IPLFRVEGTLVWIWRPLALLFTTFWIIGMLNAINFLDGLDGLAAGVCLIAAVFFAVHS
                     FRLGQSTVPLFPLALAGALLGFLPFNFSPARIFLGSAGAYFLAYQVATLSILSPAKLS
                     TALLVLAVPIIDVAWQIVSRLRQGRHPMSSDRGHLHFRLADSGLPTPRIVLGYYSVAL
                     VFGLMAVLVSSPLLKLVLLAALAVGVLALLLRLSKRT"
     gene            2373..2861
                     /locus_tag="PK829_02045"
     CDS             2373..2861
                     /locus_tag="PK829_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU40026.1"
                     /translation="MEEQDIMREQAQILIDRAYRHQQQGELGDAIALYRRSLAVYPTA
                     EAHTYLGWAYSYMDRYDEAIAECEKAIALDPTFGNPYNDIGAYMLAQGRPEEAVPWLE
                     RATAAPRYDTPQFAYLNLGRTYEALGHPYRALACYDRAQAIAPLYLPAQWAKGLLLGR
                     LN"
     gene            2880..3356
                     /locus_tag="PK829_02050"
     CDS             2880..3356
                     /locus_tag="PK829_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOU40027.1"
                     /translation="MPRAIGHVTVAAVGRLRERHWQAVQDDYARRLSRYTDFHLVEVK
                     DAVGRSLPDAVATAREGEQLLAAGRGARLVLLSATGREMTSPELAAWLQGQLESYGRL
                     AFLVGGPVGFDPATVTAADEQLALSRLTFPHELARVVLLEQLYRAFTILNGEPYHK"
     gene            complement(3363..4037)
                     /gene="rsmI"
                     /locus_tag="PK829_02055"
     CDS             complement(3363..4037)
                     /gene="rsmI"
                     /locus_tag="PK829_02055"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU40028.1"
                     /translation="MLYLVATPIGNLGDISLRALEVLRAVDYVASEDTRKTGLLLKHF
                     DIKKPQIAFHEHNEERAGARIMTLLADGQSVAVVSDAGTPGVADPGFTLVRRAIALDL
                     PVTMIPGASALVMALVLSGLPSHSFTFRGFPPRKSGKRQAFLAIDRDAPHTLIFYESP
                     HRLRDFLADALAVYGDRPAAVANELTKMFESVRRGLLSELIASLADEEPRGEYVVAIG
                     GATAAG"
     gene            complement(4052..5395)
                     /gene="aroA"
                     /locus_tag="PK829_02060"
     CDS             complement(4052..5395)
                     /gene="aroA"
                     /locus_tag="PK829_02060"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOU40029.1"
                     /translation="MTSNSKLIVHTQNTPLRGTITVPGDKSISHRAVLLAALAEGVSV
                     IRNWLPAGDTLATLEVMRALGVHIDVDERLPQAWDLRIEGRGLRGLRRPAGPLNCRNA
                     GTAIRLLAGVMAGQSFPSVLDGSEQLRRRPMGRVTEPLQLMGADIRAEEGHAPLYIRP
                     STLHGVSYSLPVASAQVKSALLLAGLYTGDAVRVFQPGPARDHTERLLMAMGAVVTMQ
                     AGWATLSDAPPGGRRLLPLDMTVPGDFSSAAFPIVAATIVPHSEVTIRGVGINETRTG
                     LLHVLRQMGANIAISNQQVTGGEVAADVSVGFAELHGVDVDGPTVVRAIDEFPVWAVA
                     ATQAVDHSTVRDAAELRVKEVDRIALLAQELRRLGANLDEHQDGFTVYGPTRLKGASV
                     DSHDDHRLGMALAVAGLATDEPTLIHDAACMADSFPGFVDTMQQLGASMEWVKPA"
     gene            complement(5406..6260)
                     /locus_tag="PK829_02065"
     CDS             complement(5406..6260)
                     /locus_tag="PK829_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HOU40030.1"
                     /translation="MDKPGRNDPCYCGSGKKYKQCHMPADLAAEREQRAWADAARNLR
                     ADLLEFADSERFDDDAGAAAAGYWNNLYTAETLAQMSASESERFFDWFAFDYALPSGE
                     RVVEQYRGEHSDLPSPQRELLDQWVAAGPMSGYELLGYERQTLRLRDMASGQEVDVFE
                     PAGHGDAPLGAILLGRLVPVQDHLEFFALPAYIPPEEIADLPAQLAALQADGGPEALR
                     GGNTLFIHHALEQAKLAGRPPVARLDPRHNASAAQQRHRHERARFKGPNGRAETLPHM
                     AQTRRKAI"
     gene            complement(6382..9609)
                     /locus_tag="PK829_02070"
     CDS             complement(6382..9609)
                     /locus_tag="PK829_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HOU40031.1"
                     /translation="MPTALRRLFSIQPGEEQLTLLLFAQMFLIGIGNNFVETSVFPLF
                     LSTFSAGTLPYLYIINAVVVALLSAAYLRLGRRLTFTQGLRVLLGFLAALTLAYWLAL
                     TLDGGRPVTFALPILFQIAVNLGQVAFWTMAGRLLTLRQSKRLFGPIGAGQWVAIVLT
                     GFLIPGIVRAIGTVNLLLVGAFGFAGALALLVVITGRYRGALDTVEAPGTVARKEAPG
                     AFLRNPYILLMFGLTIASWLSFFFVDNIFFNRISARFPDPADLSAFMGLYLAVLGIFT
                     LFNNTFLAGLIINRLGMRVGLFVLPGLLFVVTLAFSVAGVGWGLVPLLFWLATLNRVL
                     DLGFMFSVDQTAQAILYQPLPAAERARVQTIDNGIVRMVAVGLAGVSLLLLNRLPQFT
                     VVHVAMVLLVIIIAWLAVAFRTTRAYPRALMAALSRRRLTGVELSLGDSDSIAVLTEA
                     LRSPRPGSALYALGLLAQHRPEALAEAAPDLLRHPEAAVRREAARQIEALGLPAGRDA
                     LLAAAESDPDPLVRGAAWQALAAVGGAEMEPALVAHLAAPEPETRLGALLGLRHYGSD
                     TARTEAEATLAALATSANPAERRLAIRALERPAGAGCALLSRLLADSDDGVRREALAV
                     AGRAQCPAVWPEVVAALGRRATQMAAVAALRAGGEAAQPAVVSALCQNDQPVELLVGL
                     ARAAGRMGNEAVTTALAARLDHPSDTVRTEVLLALTGASYRATGEAAAAVRRQIAAET
                     SHAAWLLAGLRDCLAAEELDYLRRVLQDQFAAARDRIYLLLALLVEPSAMHDVREVLG
                     QGHQATEARRAYALEILELGLPAELKAMVMPLSEEMSPAARLQRLGGLYDAPALSPTE
                     RVEALLRGDGRANHWLEVTRLFAAGQVGCTGPDLPELLRRYQTSSDPVLRETAVWTSA
                     RLGHAPAVAPSPGERAMLTTIEKVIFLKDVDLFAETPDELLAEIAGLLTEVERPAGAS
                     VFGKGDAGDSLYIIVSGEVRIHDGDYMLNTLGESEVFGEMALLDPEPRMAAATAETDT
                     LLLRLEQSPFYELMDTRSEVARGVIRVLSRRLRQRVQEVAALRSA"
     gene            complement(9627..10037)
                     /locus_tag="PK829_02075"
     CDS             complement(9627..10037)
                     /locus_tag="PK829_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOU40032.1"
                     /translation="MTEHARVLIAKPGLDGHDRGAKVTARALRDAGMEVIYTGLRQTP
                     EMVVEAALQEDVDAIGLSILSGAHMPLTSRILELMAANDMGDVPVLLGGIIPDEDATR
                     LREMGVAAVFGPGASLDDVIAAFRAAIAARRGNA"
     gene            complement(10080..>11101)
                     /locus_tag="PK829_02080"
     CDS             complement(10080..>11101)
                     /locus_tag="PK829_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40033.1"
                     /translation="EDFLGHLRARGYLNPAALGTLELHPEFFADEATALRFLRVLRAY
                     GIGRVSVGYQASDDALLRATKRRHTAGFLPAAMALLRAEGFLVNLDLMYGLVGQSHAA
                     WATTLADAVAAAPDSISTYFLFVDRGTGLYARVQRGAAQLPGHRHAQTQHLLAQLCLE
                     AAGYHELPNDFWARPAADPATFRPERLPSQAVTLPIGPGAYGYYSGAQVGNVFDLAEY
                     ERRAAVGQSPLWRAYRLSADESFHRDVMFSLKNDPYIDCSLFRSAYNSSPLERFAARF
                     ERLERLGLVAIDGERIRLTGKGRLCVEEIAALFRAPGISPAADAAEPLLQKHLFAATY
                     PVVDW"
BASE COUNT         1787 a         3834 c         3789 g         1691 t
ORIGIN      
        1 ctccatcgcc gggtagagga tctcctccac cgggcggctg aggcggtgga cttcatcgat
       61 gaacaggatg tcgccggtgc gcatgttgga caggatggcc gccaggtcgc cagagtgctc
      121 aatggccggg ccggcggtgt gacggatgtt ggcgttcatc tcgttggcca cgacctgggc
      181 cagggtcgtt ttgcccaggc cgggcgggcc gtggaagagg acgtgatcga gtgtctcgcc
      241 gcgggcgcgg gcggcctcga tgaggatacg caaattgtcc ttgagtcgat cctgaccggt
      301 aaactgggtc aggcgttgcg gccgcagcat ggtgtccagc ccgacatcgt cggcctgctg
      361 ctgtggggcg atagcccgct ccggcgggtg cgattggctg gtcatggtgc ggggattata
      421 cgcgatctgg gggcgcgggc gaaacggcac ggccgggcta attgccgagt tggcagaaga
      481 cctcacgcaa aggacgcgaa ggaagctaag gacgccaaga agcggcttgt ttgcgccttt
      541 gccttcttgg cgatcttggc gtgaggtgtc tttatggcct ggcaatgacc ctgaatggcc
      601 cacgcacaag ctgcgaattc ggcgggagtt ggttacaatg ggacaaatca acggagtctg
      661 tcccctatgg tctttgagtc acatcacccc ctcgtcaagc acaagctgac gctgatgcgc
      721 gacagcacaa cgaagcctaa gaagtttcgc gaactcatcc gcgaaatttc cctgctcctc
      781 tgctatgaag ccaccaccga tctgacgacg cagccgcgaa cggtggacac gcccatgggc
      841 cgcgccgacg gcgttgaact gctgcacaag gtcggcctgg tgcccgtgct ccgcgccggg
      901 ctgggcatgg tcgagggcat ctgggagatg atgcccggcg cggaggtctg gcacatcggc
      961 ctctatcgcg acgagcgcac gctgaagccg gtgtcctact acaacaagct gccgaccatg
     1021 cccacggtgc aggtctgcct ggtgctcgat ccgatgctgg ccacgggcgg ctcggccgtg
     1081 gccacggtgg acatcctcaa gcgctggggc gcggagcgca tcaagttcgt cggcatcctg
     1141 gccgcgccgg agggcatcgc ccgcctgcaa ggagcgcacc cggacgttcc cattcacgtc
     1201 gcccacgtcg atgagcggct caatgacatc ggctacattg tccctggcct gggcgacgcc
     1261 ggcgaccggc agttcggcac agggtaacgt ggtcgccttc gtcgtcaccc tggccgcgct
     1321 ggtcgtcgcc gcggcgacca cgccgctggc ccgccgcctg tccttccgtt ggggcatcgt
     1381 cgctgagccg ggcggccgtc gccagcacca cggccgcatg cccaagctgg gcgggttggc
     1441 cgtggccgcg ggttgggccg ttggcgtggc cctcatctac ctgctgcggc cgcccggcaa
     1501 ccccgacgac gccctgcggt tgcgcggcgt ggtgttgggc agcctgatcg tgctggccgg
     1561 cggcctgctc gacgaccgct acgacctgcc gccgtgggcg caactggtga tccagttgtc
     1621 cggcgcgctg gtggccatcg cccacatcat cttcatcgaa gtcttcacca acccactgcc
     1681 cggcacggca ttgtggcagg caattcccct ctttcgcgtc gaggggacac tggtgtggat
     1741 ctggcgtccg ctggcgttgc tcttcacaac cttctggatc attggcatgc tcaacgccat
     1801 caacttcctg gatggccttg acgggctggc ggccggggtg tgcctgatcg ccgcggtctt
     1861 cttcgccgtc cacagcttcc ggctgggcca atcgaccgtg ccgctctttc cgctggcact
     1921 ggccggggcg ctgctgggct ttttgccgtt caacttctcg ccggcgcgca tctttctcgg
     1981 ctcggccggg gcctactttt tggcttatca ggtagccacg ctgtcgattc tctcgccggc
     2041 caaactgtcc acggcgctgc tggtgctggc cgtgcccatc atcgacgtgg cctggcaaat
     2101 cgtctcgcgg ctgcggcaag ggcggcaccc catgagcagc gaccgcggcc acctgcactt
     2161 tcgcctggcc gacagcggcc tgccgacgcc gcgcattgtc ctgggctatt acagcgtcgc
     2221 cctggtgttt gggctgatgg ccgtcctggt gtcctcgccg ctgcttaagc tggtcttgct
     2281 ggcggcgctg gccgtgggcg tgctggcttt gttgctgcgg ctgagtaaga ggacttgaat
     2341 cggccgccgc ccggtgctac actcgcacta tcatggaaga acaagacatc atgcgcgaac
     2401 aggcgcaaat cctgatcgat cgcgcctatc gccaccagca acagggcgaa ctgggcgacg
     2461 ccatcgccct ctaccggcgg tcgctggccg tctacccgac ggccgaagcc cacacctatc
     2521 tcggctgggc ctatagctac atggatcgct acgacgaggc catcgccgag tgcgagaagg
     2581 ccatcgctct cgatccgacg ttcggcaacc cctataacga catcggcgcg tatatgctgg
     2641 cccaggggcg gccggaagag gccgtgccct ggctggagcg ggccacggcc gcgccgcgct
     2701 acgacacacc gcagttcgcc tacctcaacc tcggccggac ctatgaggcc ctgggccacc
     2761 cctaccgcgc cctggcctgc tacgaccgcg cccaggccat cgccccgctt tacctgcccg
     2821 cccagtgggc caaggggctg ctgctcggcc gcctgaacta ggctgaggcc ctggccccca
     2881 tgccccgcgc catcggccat gtgacagtcg ccgcggtcgg ccggctgcgc gaacgccact
     2941 ggcaggcggt gcaggacgac tacgcccgcc gcctgagccg ctacaccgat tttcatctgg
     3001 tcgaggtcaa agatgcggtt ggccggtcgc tgcccgacgc cgtggccacg gcccgcgagg
     3061 gggagcagct cctggccgcc gggcgcggcg cgcggctggt gctgctgagc gccacggggc
     3121 gagagatgac cagcccggag ttggcggcgt ggctacaggg gcaactggag agctacggcc
     3181 ggctggcctt cctcgtcggc gggccggtcg gcttcgatcc ggcgaccgtg accgcggccg
     3241 acgagcagct ggccctctcg cggctgacat tcccccacga actggcccgc gtcgtcttgc
     3301 tggagcagct ttaccgggcg tttacgatcc tcaatgggga gccgtatcac aagtaggcgg
     3361 cgctacccgg ccgcggttgc cccgccgatc gccaccacgt actcccctct tggctcctcg
     3421 tcggccaacg aggcgattag ttcggagagc aagccccgcc gtaccgactc aaacatcttc
     3481 gtcaactcgt tggccacggc cgccggccgg tcgccataga ccgccagcgc gtcggccagg
     3541 aagtcgcgca gacgatgggg cgattcgtag aagatgagcg tgtggggcgc atcgcggtcg
     3601 atggccagga acgcctgccg cttgccgctc ttgcgcggcg ggaaaccgcg aaaggtgaag
     3661 ctgtgcgacg gcagcccgga cagcaccagg gccatcacca gcgccgacgc accgggaatc
     3721 atcgtcactg gcagatcaag cgcgatggcc cgccgcacca gcgtgaagcc ggggtcggcc
     3781 acgcccggcg tgcccgcgtc ggagacgacg gcgaccgatt gcccgtccgc cagcagggtc
     3841 atgatgcgcg ccccggcgcg ctcctcgttg tgctcgtgga aggcgatctg cggcttcttg
     3901 atgtcgaagt gcttcagcag caggccggtc ttgcgcgtgt cctcactggc gacgtagtcc
     3961 acggcgcgca gcacctccag cgcccgcagg ctgatgtcgc cgaggttgcc gatgggcgtc
     4021 gccaccaggt agagcaccgc ccctccacgg cctaggccgg cttaacccac tccatcgacg
     4081 cccccagttg ctgcatcgta tcgacgaagc cggggaagct gtcggccatg caggcggcgt
     4141 catggatgag cgtcggctcg tcagtcgcca gcccggccac ggccagagcc atgcccaggc
     4201 ggtggtcgtc gtggctatcg acgctggccc ccttgaggcg cgtcggcccg tagacagtga
     4261 aaccgtcctg gtgctcatca aggttggctc ccagacggcg caactcctgg gccagcaggg
     4321 caatgcgatc cacttccttg acgcgcaact cggccgcgtc gcggacggtg ctatgatcga
     4381 ccgcctgcgt ggcggccaca gcccagacgg ggaactcatc gatggcgcgt acgaccgtcg
     4441 gcccatccac atcaacaccg tgcagctcgg cgaagccaac gctgacgtcg gcagccactt
     4501 cgccgccggt cacctgctgg ttgctgatgg cgatgttggc gcccatctgt cgcaggacgt
     4561 gcagcaggcc ggtgcgcgtc tcgttgatgc ccacgccgcg gatggtgacc tcggagtggg
     4621 gcacgatggt ggcggcgacg atggggaagg cggccgagga gaaatcgccg ggcacggtca
     4681 tgtccagcgg cagcaggcga cggccgcccg gcggcgcgtc gctcagcgtc gcccagcccg
     4741 cctgcatcgt cacgaccgcg cccatggcca tcagcaggcg ctcggtgtgg tcgcgggccg
     4801 ggccgggctg gaaaacgcgc acggcgtcgc cggtgtagag gccggccaac agcagggcgc
     4861 tcttcacctg ggcgctggcc acgggcaagc tatagctgac accgtggagc gtcgacgggc
     4921 gaatgtacag cggcgcgtgc ccttcttcgg cgcggatgtc cgcgcccatg agttgtagcg
     4981 gctcggtcac gcggcccatc ggccggcggc ggagctgctc gctgccgtcc aacaccgagg
     5041 ggaacgattg cccggccatt accccggcca gcaggcggat ggccgtgccg gcgttgcggc
     5101 agttgagcgg cccggccggc cggcgtaggc cgcgcaaccc ccgcccctca atgcgcaagt
     5161 cccacgcctg cggcaggcgc tcatccacgt cgatgtggac acccagcgcg cgcatcactt
     5221 ccagcgtcgc cagcgtgtcg ccggccggca gccagttacg gatgacgctg acgccttcgg
     5281 ccagcgcggc caacaggaca gcgcgatggc tgatcgactt gtcgcccggg acggtgatcg
     5341 tgccgcggag cggggtattc tgggtatgga cgatcagctt actgtttgac gtcattgggg
     5401 ccggcttaga tggctttgcg ccgtgtctgg gccatgtgcg gcagggtttc ggcgcggccg
     5461 ttggggcctt taaaacgcgc gcgttcgtgc cgatggcgct gctgagcggc gctggcatta
     5521 tggcgcgggt cgaggcgggc aaccggcggc cgcccggcca gcttggcctg ctccagcgcg
     5581 tggtggatga agagggtgtt cccgccgcgc agagcctcag gcccaccgtc ggcttgcagc
     5641 gccgccagtt gcgccggcag gtcggcgatc tcttccggcg ggatgtaggc cggcagggcg
     5701 aagaactcca gatggtcctg gacgggcacg aggcggccaa ggaggatcgc ccccagcggc
     5761 gcgtcgccat ggccagccgg ctcaaagaca tcgacctcct gcccggaggc catgtcgcgc
     5821 aggcgcaacg tctgccgctc gtagcccaac agctcgtaac cgctcatcgg cccggcggca
     5881 acccactggt caagcagttc gcgctgtggc gaaggcaggt cgctgtgctc gccgcgatat
     5941 tgctcgacga cgcgctcacc cgacggcaag gcgtagtcga aggcgaacca gtcaaagaag
     6001 cgctcggact cggaggcgct catctgtgcc aacgtctcag ccgtgtacaa gttgttccag
     6061 tagccggccg cggccgcgcc ggcgtcgtcg tcgaagcgct cgctatcggc gaactccagc
     6121 aggtcggcgc gcaaattgcg cgcggcgtcg gcccaggcgc gctgctcgcg ctcggcggcc
     6181 aggtcggcgg gcatgtggca ctgcttgtac ttcttgccac tgccgcaata gcagggatcg
     6241 tttcggcccg gtttgtccat atcgcccaac tccataagat ggcaaccggc aacgccagag
     6301 atgccggctg cgaaatgaca aggaattgta ccagatgaag ccaccgccgc gtagcgtcag
     6361 cgaaccagcc acccgccgcg actaggccga ccgcagcgcg gccacctcct gcacgcgctg
     6421 ccgcagccgc cgcgagagca cgcggatgac accgcgggcc acttcgctgc gcgtgtccat
     6481 cagctcatag aacggcgatt gctccaggcg cagcaacagc gtgtccgtct cggccgtggc
     6541 cgcggccatg cgcggttccg ggtccagcag ggccatctcg ccgaagactt ccgactcgcc
     6601 cagggtgttg agcatgtagt cgccgtcgtg gatgcgcacc tcgccgctaa cgatgatgta
     6661 gagcgagtcg cccgcgtccc ccttgccgaa gacgctcgcg cccgccggtc gctccacctc
     6721 ggtcaacagc ccggcgatct cggccagtag ctcgtccggc gtctcggcga agagatcgac
     6781 gtccttcagg aaaatgacct tctcaatcgt cgtcagcatg gctctttctc cgggtgacgg
     6841 ggcaacagcg ggggcatgac ccagacgggc ggacgtccag acggccgtct cgcgcaagac
     6901 gggatcgctg ctggtctgat agcgccgcaa tagttcgggc aggtcggggc cagtgcaacc
     6961 cacctgcccg gcggcgaaca ggcgcgtgac ctccagccag tggttggccc ggccgtcgcc
     7021 gcgcagcagc gcctcgacgc gctccgtggg cgacagagcg ggcgcgtcat acagaccgcc
     7081 caggcgctgc aaccgcgccg cgggcgacat ctcttccgac agcggcatga ccattgcctt
     7141 tagctctgcc ggcaagccca actcaaggat ctccagcgcg taggcccggc gcgcctccgt
     7201 ggcctggtgg ccctgaccca acacctcgcg cacatcgtgc attgcggacg gctccaccag
     7261 cagcgccagc agcaggtaga tgcggtcgcg ggcagcggcg aactggtctt gtagcacgcg
     7321 ccgtagatag tcgagttcct cagcggccag gcagtcgcgc aggccggcca gcagccaggc
     7381 ggcgtggctg gtctcggcgg caatttggcg gcgcacagcg gccgcggcct cgcccgtggc
     7441 ccggtagctc gcgcccgtca gcgccagcag cacttccgtc cgcaccgtgt cgctgggatg
     7501 atccagccgg gccgccagcg ccgtcgtgac cgcttcgttg cccatgcgcc cggccgcgcg
     7561 ggccaggccc accaacaact ccaccggctg gtcgttctgg cagagggcgc tgacgacagc
     7621 cggctgcgcc gcctcgccgc ccgcccgcaa tgcggccacg gccgccatct gcgtcgcccg
     7681 gcggcccagc gcggcgacaa cctcgggcca gacagcgggg cactgcgccc gcccggcgac
     7741 ggccaacgct tcgcggcgca cgccgtcgtc gctgtcggcc agcaggcgcg atagcagggc
     7801 acaaccggcc ccggccggcc gctccaacgc gcgaatggcc agacggcgct cggcgggatt
     7861 ggccgaagtc gccagggcgg ccaacgtcgc ctcggcctcc gtccgtgccg tgtctgagcc
     7921 gtagtggcgc agacccagca gcgctcccag ccgcgtctcc ggctccggcg cagccagatg
     7981 cgccaccagg gccggctcca tctccgcccc acccacggcc gccagcgcct gccaggccgc
     8041 gccgcgcacc agcgggtcgg ggtcgctctc ggccgcggcc agcagggcgt cgcgcccggc
     8101 cggcaggccc agcgcctcga tctgccgcgc ggcctcgcgg cggacggcgg cttcgggatg
     8161 gcgtagcagg tcgggcgcgg cctcggccaa cgcctccggc cggtgctggg ccagcaaacc
     8221 gagcgcgtag agggccgaac cgggccgggg gctgcgcagc gcctcggtca gcacggcgat
     8281 gctgtcgctg tcgcccaggc ttagctctac gccggtgagc cgccgccgcg acagcgcggc
     8341 catgagcgcc cgcggatagg cgcgcgtcgt ccggaaggcc accgccagcc aggcgatgat
     8401 gatgaccagc agcaccatcg ccacgtggac gacggtgaac tgcggcaggc gattgagcag
     8461 cagcagcgac acgccggcca atcccacggc gaccatgcgc acgatgccgt tgtcgatggt
     8521 ctggacgcgc gcccgctcgg ccgcgggcaa cggctggtag aggatggcct gcgccgtctg
     8581 atcgaccgag aacatgaagc ccaggtcgag gacgcggttg agcgtggcca gccagaagag
     8641 cagcggcacc agcccccagc cgacgccggc gacgctgaag gccagcgtga cgacgaacag
     8701 caaccccggc agcacgaaca gccccacgcg catgcccaga cggttgatga tcagtccggc
     8761 caggaaggtg ttgttgaaca gggtgaagat gcccagcacg gccaggtaga ggcccatgaa
     8821 ggccgacaga tcggccggat cgggaaagcg cgcgctgatg cggttgaaga agatattgtc
     8881 aacgaagaag aacgacagcc acgaggcaat cgtcaggccg aacatgagca ggatgtaggg
     8941 gttacgcagg aacgcgcccg gcgcttcctt gcgggcgaca gtgcccggcg cctcaaccgt
     9001 gtccagcgcc ccgcggtagc ggccggtgat gaccactagc aacgccagcg ccccggcgaa
     9061 gccaaacgcg cccaccagta gcagattgac tgtgccgatg gcgcggacga tgcccggaat
     9121 gaggaagccg gtcaggacaa tggccaccca ctggccggcg ccaatcggcc cgaagaggcg
     9181 cttcgactgg cgcaacgtca acagccgccc ggccatcgtc cagaaggcga cctggcccag
     9241 gttcacggcg atctggaaga ggatgggcag ggcgaaagtg accggccggc cgccgtccag
     9301 cgtcagcgcc agccagtagg ccaacgtcag cgccgccaga aagcccaaca gcacgcgcag
     9361 cccctgggtg aacgtcagcc gccggcccag gcgcaggtaa gcggccgaca gcagggccac
     9421 aacgacggcg ttgatgatgt acaggtaggg cagcgtgccg gcgctgaagg tgctcaggaa
     9481 gagcgggaag acggacgttt cgacgaagtt gttgccgatg ccgatcagaa acatttgggc
     9541 gaagagcagc aaggttagct gctcttcgcc cggctggatg ctgaagagac ggcgcagagc
     9601 ggtgggcatt ggtcggatag atagactcag gcgttgcccc ggcgagcggc gatggcggcg
     9661 cggaaggcgg cgatcacgtc gtcgaggctg gcccctggcc cgaagaccgc ggccacgccc
     9721 atctcccgca gccgggtcgc gtcctcgtcg gggatgatgc cacccagcag cacgggcacg
     9781 tcgcccatgt cgttggcggc cattagttcc aggatgcggc tcgtcagggg catatgcgcg
     9841 ccggagagga tgctcaggcc aatggcgtcc acgtcctctt gcagcgccgc ctcgacgacc
     9901 atctccggcg tctggcgcaa accggtgtag atgacctcca taccggcgtc gcgcagagcg
     9961 cgggcggtca ccttggcccc acggtcgtgg ccgtccagcc ccggcttggc aatgagcacg
    10021 cgcgcgtgtt cggtcattcg ttcaacctcc cggacaggag tctgccaccc cgccccacgt
    10081 taccagtcca ccaccggata tgtcgccgcg aagagatgct tttgcaacag cggctcggcc
    10141 gcgtcggccg ccgggctgat gcccggagcg cggaacaggg cggcgatctc ctcaacgcac
    10201 agccgcccct tgccggtcag gcggatgcgc tcgccatcga tggccaccag gcccagccgc
    10261 tccagccgct cgaagcgcgc cgcgaagcgc tccagtggac tactattata ggcgctgcgg
    10321 aacagactac aatcgatata gggatcattc ttcagagaaa acatcacatc gcggtgaaat
    10381 gactcgtcgg cgctgaggcg ataggcgcgc cacagcggcg attgacccac ggccgcgcgc
    10441 cgctcgtact cggccaggtc gaagacgttg cccacctgcg cgccgctgta gtagccgtag
    10501 gcccccggcc cgatgggcag cgtcacggcc tgcgacggca gcctttctgg ccggaaggtc
    10561 gccgggtcgg ccgccgggcg cgcccagaag tcgttgggca actcgtggta gccggccgcc
    10621 tccagacaca actgggccag caggtgttgc gtctgcgcgt gccggtggcc tggcaactgc
    10681 gccgcgccgc gctgcacgcg cgcatacagc cccgtgcccc ggtcgacgaa caggaagtag
    10741 gtgctgatgg agtctggcgc ggcggcgacg gcgtcggcca gtgtcgttgc ccaggcggcg
    10801 tggctctggc cgacgaggcc atacatcagg tcgagattga ccagaaagcc ctccgcccgc
    10861 agcagagcca tcgccgcggg caggaagccg gccgtgtgcc gccgctttgt cgcccgcagc
    10921 agcgcgtcgt ccgaggcctg gtagccgacg ctgacccggc cgatgccgta ggcccgcagc
    10981 acgcgcagga aacgcagcgc cgtggcctcg tcggcgaaga actccgggtg cagctccagc
    11041 gtgcctagcg cggccgggtt caggtagccg cgcgcccgca gatgacccag gaagtcctcc
    11101 a
//
LOCUS       DAOSMQ010000034        11058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5889_length_11058_cov_4.793965, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000034 DAOSMQ010000000
VERSION     DAOSMQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5889_length_11058_cov_4.793965"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PK829_02085"
     CDS             complement(<1..1011)
                     /locus_tag="PK829_02085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU40034.1"
                     /translation="MAARTIGRYVVREEIGQGGMATVYRALDPHFQRDVAIKVLPLHF
                     LHDPRFRARFEREARIVAGLQHPAIVPVYDFGEDNGQPYLVMALMSGGSLEDRLRQGP
                     MPLVETERIIGRLAPALDAAHQRGVIHRDLKPANILFDQWNEPYLTDFGIVKLTQGSS
                     DDSASLTTVGGIIGTPAYMSPEQVQGAVIDGRSDIYSLGVILFQMLSGRLPYDATTPI
                     GLAFKHISEPIPSIQAMGNVRLPAEFQTIVNQAMAKLANDRYPTAVALARDLTRVVHG
                     SGPRPTVVVPPAPGGLAATQRVEGPPSAPQMPSGGPPAYPSGWPAVPPQAAAPPQAAA
                     PTA"
     gene            complement(1127..1573)
                     /locus_tag="PK829_02090"
     CDS             complement(1127..1573)
                     /locus_tag="PK829_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOU40035.1"
                     /translation="MSAEHATALAQALQARGYRLTPARRAILDALAAAGDHPTADELA
                     AAVVRAAPGVGRMTVYRTLDLLSELGLLRPVYQGTGAARGARHYVLLLDGHHHHLVCS
                     CCRRVIEFDDCALGELAETVARRYGFVVEGHLLEFYGRCAECGSRD"
     gene            complement(1570..2409)
                     /locus_tag="PK829_02095"
     CDS             complement(1570..2409)
                     /locus_tag="PK829_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOU40036.1"
                     /translation="MSFLLAPLQYDFIVRALVAAIIVGVVCSVLGVYIVLRGMAFLGD
                     AMSHTILPGVVVAYFLGWPLAVGALVMGVLTALGIGFLTRRAALQEDTAIGVIFAGFF
                     ALGVALLSAGASSVDLAHFLFGNLLGVSSGDLLITAALGGLVLLVIFLFYKEFLVLSF
                     DPLLATTLRLPTTFLHYLLLLLIAITIVVALQVVGVALMLAILVTPAAAASFVTHRLP
                     AMMAVSALIGVVSGVAGVYAGYYLNIASGPAVVLAATLIFLIVLTFSVLRRRSPAAEG
                     VAL"
     gene            complement(2482..3312)
                     /locus_tag="PK829_02100"
     CDS             complement(2482..3312)
                     /locus_tag="PK829_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOU40037.1"
                     /translation="MTGPHPQHGIAHEPDAPTLEADDVWAVYRDRLAADGPRYALEAV
                     TFRVARGERVAIVGPNGAGKSTLFKLIVGTLRPDRGRLDVFGHGPAGHICIAYVPQRA
                     QVDWRFPVTVEDVVMMGRVGQIGLLRRPGRADWTATRAALARVGAAALARKQIGGLSG
                     GQQQRVFLARALAQEAELVLLDEPLNGLDAPSQEAILAVLDDLRRDGVTVLLATHDLD
                     LAAERFDRIMLLNRRVIAFGPPAAVLQADTLLRAYGGHLHVIDADGTAIIADPSCDEN
                     "
     gene            complement(3309..4412)
                     /locus_tag="PK829_02105"
     CDS             complement(3309..4412)
                     /locus_tag="PK829_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOU40038.1"
                     /translation="MPGRIGIWLAGALLAAGLLGCGGQAAEPATTNVATPADALAPLS
                     LPAVAAVPGEGPLRVVATTSIIGDIAGRVGGEAIALTTLMAAGQDPHSYQPAAADLAA
                     AADADLIFVNGWNLEEGLIADLDAAGDAPRVPVSAGITPLPSSADPRAADPHVWQDVA
                     NVMQWVDNIAATLSAARPADAATFTANAAALRAELATLDEDIHAATAAIPATRRILVT
                     NHDTLAYFAAAYDFRVLGSVIPGASTLAEATAANLAALADAMNDTGICSLFIETTAAD
                     QLARALSEELDGCNTVRVLSLYSDALGPPGSGADSYTGMMRANVATIVAALGADEQGS
                     GGAGVRGRKGAGTVCSPLPPCPPAPLLSNRRPT"
     gene            4910..7174
                     /locus_tag="PK829_02110"
     CDS             4910..7174
                     /locus_tag="PK829_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU40039.1"
                     /translation="MDAPLPDTSVQPAEPAVEPSRDPRPPPGSLTADTPFEAARRLER
                     RQQVALTILSAVAPATAINIAVGYIEEAIDCLAAGISLYSAFPETMTVLQDSGDGWEP
                     GTQVPVTRTDILATVAAGDLYYLADLRAASAGSPGFQHALAMGGRSLLAAPVRADGQL
                     LGVLWIITGDPRPFSSDEETVAREVANVVAVALHNRRLLAAEQKARARERSLREVGAS
                     LTLGLSRGTVLKRILEQLGELIPFASASIVLLDGGEIVFAAAHELALRPEQVDDLRTH
                     TPPILRALIDTRAPRMIDDTATDPDWLPLPGFSYIRAWLGVPLLFNGVCIGALTIDRE
                     QPRSFSAADVELASAFANQAAVAIENARLFNEAQRYAEQLEARVRERTRELEALYGIS
                     AATIERPEMGNVLARALELVVDAFDCPAATVHLIQPGQPELPLIAHLDRSGSHLASLL
                     GAPPVAALLRRQALGGAVRIAGDELATALAADRAWAAASAPLRAHGRNLGVLSLWSDS
                     PDAFEGSMRFLTAVADQLGAALENLQLRQMTRQAAVLEERERMARELHDAVTQTIYSA
                     GLLAEAARESAHAGDLRAVESHTQIIVQRVYQALREMRLLLFELRADTVAWQGLAIAL
                     RTRLQAVEERANIATQLSVKDVSELPPALEEAFYRVALEALNNALHHARAGRVSVTLR
                     VAGGELILVVRDDGVGFRRRDAAAGSGMGLESMARRLDKVGGRLSITSRQGRGTRVEA
                     RAPLTAGPQEAEQQ"
     gene            7171..7836
                     /locus_tag="PK829_02115"
     CDS             7171..7836
                     /locus_tag="PK829_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU40040.1"
                     /translation="MTDAIHILVVEDQTIVREGLCAMLATKPGLRVIGTAADGEEAVH
                     RALALRPDVILMDLIMPRMDGIRATREIVAQWPAARILILTSYSDDAQIFEAFHAGAL
                     GFVLKQDVGQKLVEALQQIHAGQSPLNSTVTLRLMQSLAIRNTATPREATLETQLTER
                     ELEIARLVARGYDNQNIADALNISVRTVGTHVSNILRKAGVSNRTQLARLMLRQGLAS
                     LYE"
     gene            complement(7880..8734)
                     /locus_tag="PK829_02120"
     CDS             complement(7880..8734)
                     /locus_tag="PK829_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU40041.1"
                     /translation="MSKKKVAIITDSVANLTPETLAEHDIYVIPQILNWEGNSLLDQI
                     DITTTEFYERLPKSKDLPKTSQPSPGQFTEHFMKVAAGADSIVAVFVSSALSGTIQSA
                     HLGAQAMGDYPIEIVDSRSASLGLGLLAVAAARHAAAGHDTHAVAEYVRGLVPRMRVL
                     FVVDTLEYLHKGGRIGGAKRLVGSVLSIKPVLHIENGQIEPLASVRTKSKAVAHMLEV
                     VLGEMKGKKHIHAGIIHAGAYSDALAIKEQVLAAVQPDELLLNELTPVIGANVGPGIL
                     GMGYYTEE"
     gene            complement(9121..>9612)
                     /locus_tag="PK829_02125"
     CDS             complement(9121..>9612)
                     /locus_tag="PK829_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HOU40042.1"
                     /translation="LEHLRDVREAVRLPVLRKDFLFHPYQLYEARVAGADAVLLIAAV
                     LGDHDLAELHALARQLGMAALVEVHNEAELARVLPLGPELVGVNNRDLQTFAIDLGTT
                     ARLRALIPPAVAVVAESGLKTPDDVTAMRVAGAAAVLIGEALARSKDVVATTRQFVAA
                     GRA"
     assembly_gap    9613..9712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9713..10091)
                     /locus_tag="PK829_02130"
     CDS             complement(<9713..10091)
                     /locus_tag="PK829_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HOU40043.1"
                     /translation="MSVTNLAKRRGEILDEIMAHHRERLPRVMAERPLADLRAMAAVA
                     PPPRDFAAALRAPGVALIAECKKASPSKGLIVPDYDPVRLARAYEKGGARAVSVLTDA
                     RHFQGALEHLRDVREAVRLPVLRK"
     gene            complement(10242..>11058)
                     /locus_tag="PK829_02135"
     CDS             complement(10242..>11058)
                     /locus_tag="PK829_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40044.1"
                     /translation="GAAFRLRVFTVNHPGYDVPASAAIDRYHLEAYSIRHQPQAMRAG
                     LRWLLDGPLARETDIIWIAYGHSMGGLALSQCQTEALVEEMAAAGRRLRPTRVLSAPA
                     FCLQPEARAALSRLDVLHAMKLTLGRLPLYAPVATGLFRAFAPLFYLAGADRFTIGGL
                     RGFGGYGRLNPFILLEQGRELLRLEPDDVCGPDTLADAHVLLSRQDGMVNWQATHDLI
                     ATARRDGHRVRHYDLDSTHLMEVDQPQAVAGILRQIIAAALAEWPADDEDLRR"
BASE COUNT         1865 a         3626 c         3779 g         1688 t
ORIGIN      
        1 cgccgtcggc gcggcggcct gcggcggcgc ggcggcctgc ggcggcacag ccggccagcc
       61 cgacgggtag gcgggtgggc cgccagacgg catctgcggc gcgctgggtg ggccttcgac
      121 gcgctgcgtg gccgccaacc cgcccggcgc gggcggcacg accaccgtcg gccgtgggcc
      181 ggagccgtgg acgacgcgcg tcagatcgcg ggccagggcc acggctgtcg ggtagcggtc
      241 gttggccaac ttggccatgg cctggttgac aatggtctgg aactcggccg gcaggcggac
      301 attgcccatc gcctggatgc tggggatcgg ctcggagatg tgcttaaacg ccaggccgat
      361 gggcgtagtc gcgtcgtagg gcagccggcc gctgagcatc tggaacagga taacgcccag
      421 cgagtagatg tcgctgcggc cgtcgatgac ggccccttgc acctgctctg gactcatgta
      481 ggcgggcgtg ccgatgatgc cgccaacggt ggtcagcgag gccgagtcgt cgctgctgcc
      541 ctgcgtcagc ttgacaatgc cgaagtcggt caggtatggc tcgttccact ggtcaaagag
      601 gatgttggct ggcttcaggt cgcggtggat gacgccgcgc tggtgggcgg cgtcgagagc
      661 gggggccagg cggccgatga tgcgctccgt ctccaccagc ggcatcggcc cctggcgcag
      721 gcggtcttcc agcgagccgc cgctcatcag cgccatgacc aggtagggct ggccgttgtc
      781 ctcgccgaag tcgtagacgg gcacaatggc cgggtgttgc agcccggcga cgatgcgcgc
      841 ctcgcgctcg aagcgggcgc gaaagcgggg atcgtggaga aagtgcagcg gtagaacctt
      901 gatggccacg tcgcgctgaa agtggggatc gagcgcgcgg tagaccgtgg ccataccccc
      961 ctggccgatc tcctcccgca ccacatagcg cccaatcgtc ctcgccgcca tgagtttatt
     1021 gtactgcgtc cgcccttggt ggccaagagg aggggctggg gggttgggtt ccaggttcca
     1081 ggggccaggt tccagggagg agcactcttc ccctggaacc tggcccctaa tccctactcc
     1141 cgcactccgc gcagcgccca taaaactcca gcaagtgccc ttcgacgacg aagccatagc
     1201 gccgcgcgac tgtctcggcc agctcgccca gggcgcagtc gtcgaattcg atcacccgcc
     1261 ggcagcagga gcaaacgaga tggtggtggt ggccgtccag taacaaaacg tagtggcgcg
     1321 ccccgcgggc tgcgcccgtg ccctggtaga ccggccgcag cagccctagc tcgctcagca
     1381 ggtcgagggt gcggtagacg gtcatgcggc cgacgcccgg cgcggcgcgg acgacggcgg
     1441 cggccagttc gtcggccgtc ggatggtcgc cggcggcggc cagggcgtcg agaatggccc
     1501 ggcgggcggg cgtcaaccga tagccgcgcg cttgcagggc ttgcgccagc gccgtagcgt
     1561 gctcggcgct catagagcaa ccccctcggc cgcgggcgag cgccgccgca gcacgctgaa
     1621 ggtcagcacg atcaggaaga tgagcgtcgc cgccagcacc actgccgggc cggaggcgat
     1681 gttgaggtag tagcccgcgt agaccccggc cacaccggag acgacgccga taagcgccga
     1741 cacggccatc atcgccggca gccggtgggt gacgaacgaa gccgccgccg ccggcgtcac
     1801 cagaatggcc agcatcaggg ccacgccgac gacttgcagg gcgacgacga tggtgatggc
     1861 gatgagcagc aacagcaggt agtggaggaa ggtcgtcggc agccgtagcg tcgtcgccag
     1921 cagcgggtcg aacgacagca ccaggaactc cttgtagaac aagaagatga ccaacagcac
     1981 cagcccgccc agcgcggcgg tgatgagcag atcgccgctg gagacgccga gcaggttgcc
     2041 gaacaggaag tgggccagat cgaccgaact ggctccggcc gacagcagag ccacgcccag
     2101 ggcgaagaaa ccggcgaaga tgacgccaat agccgtgtcc tcttgcagcg ctgcccgccg
     2161 cgtcaggaag ccaatgccca gcgccgtcag cacgcccatg accagcgcgc cgacggccag
     2221 cggccagcct aggaagtagg cgacgacgac gcccggcagg atggtgtggg acatggcgtc
     2281 gcccaggaag gccatgcccc gcagaacaat gtagacaccc agcacggagc agaccacgcc
     2341 gacgatgatg gcggccacca gcgcgcggac gatgaagtcg tattggaggg gggcgaggag
     2401 gaaggacatg ggcaagtaag tggtcagtgg tcagtaaagc agttggtcag tagtctgtcg
     2461 tctgtggccg gcggccggcg gtcagttctc gtcgcaagat gggtcggcaa tgatcgctgt
     2521 gccatcggcg tcgatgacgt ggagatggcc accataggcg cgcagcaggg tgtcggcttg
     2581 caggacagcc gccggtgggc cgaaggcgat gacgcgccgg ttgagcagca tgatgcggtc
     2641 gaagcgctcg gcggccaggt cgaggtcgtg ggtggccagc agtacggtca cgccgtcgcg
     2701 gcgcaggtcg tcgagcacgg ccaggatggc ctcctgcgag ggcgcgtcga ggccgttgag
     2761 cggctcgtca agcagcacca actcggcctc ctgggccagg gcgcgggcca ggaagacgcg
     2821 ctgctgttgg ccgccggaca ggccgccgat ctgcttgcgg gccagcgccg ccgcgccgac
     2881 gcgcgccagc gccgctcgcg tcgccgtcca gtcggcccgc cccggccgtc gcagcagccc
     2941 gatctgcccc acccggccca tcatgacaac gtcctcgacc gtcaccggga agcgccagtc
     3001 cacctgggcg cgctggggca cgtaggcgat gcagatgtgg ccggccgggc cgtggccgaa
     3061 gacatccagc cggccgcggt cggggcgcag cgtcccgacg atgagcttga aaagcgtgct
     3121 cttgcccgcg ccattggggc cgacgatggc cacgcgttcg ccgcgggcca cccggaaggt
     3181 gaccgcctcc agggcgtagc gcggcccgtc ggcggccaac cggtcgcgat agaccgccca
     3241 aacgtcgtcg gcctccagcg ttggcgcgtc cggctcgtgg gcgatgccgt gctgtgggtg
     3301 ggggccggtc acgtgggcct cctgttggag agcagggggg caggggggca ggggggcagg
     3361 ggagagcaga ctgtccctgc tcccttgcgc ccccgcaccc ctgcgccccc gctcccctgc
     3421 tcatcagcgc ccagcgccgc cacaatcgta gcgacattgg cccgcatcat accggtgtag
     3481 ctgtccgcgc cgctgccggg cgggccgagg gcgtcgctgt agagtgacag gacgcgcact
     3541 gtgttgcagc cgtccagttc ctcgctcagg gcgcgagcca gctggtcggc ggccgtggtc
     3601 tcgatgaaca gactacagat gccggtgtcg ttcatggcgt cggccagcgc cgccaggttg
     3661 gccgccgtgg cctcggccag cgtgctggct ccggggatga ccgaaccgag gacgcgaaag
     3721 tcgtaggcgg cggcgaagta ggccagcgtg tcgtggttgg tcaccagaat gcgccgcgtc
     3781 gcgggaatgg ccgccgtagc cgcgtggatg tcctcgtcca gcgtagccaa ctcggcgcgc
     3841 aaggcggcgg cgttggcggt aaaggtggcg gcgtcggccg ggcgagcggc gcttagcgtc
     3901 gcagcgatat tgtccaccca ctgcatcacg ttagccacgt cctgccagac gtgggggtcg
     3961 gccgcacgcg ggtcggcgct ggagggcaag ggcgtgatgc cggccgacac gggcacgcgt
     4021 ggcgcgtcgc cggcggcatc gaggtcggca atgagtcctt cctccaggtt ccagccgttg
     4081 acgaagatga ggtcggcgtc ggccgccgcg gccagatcgg ccgccgccgg ctggtagctg
     4141 tgggggtcct gcccggcggc catgagcgtc gtgagggcaa tggcctcgcc gccgacgcgg
     4201 ccggcgatgt cgccgatgat gctcgtcgtg gccaccacgc gcagcgggcc ttcgccgggc
     4261 acagcggcca cggccggcaa ggacagcgga gccaacgcat ccgccggcgt ggccacgttt
     4321 gtagtcgccg gctcggccgc ctgtccgccg cagcccagca gccccgcggc cagcagcgcc
     4381 ccggccaacc atattcctat ccgtcctggc acgtcgttca aagcactcct aattgatatt
     4441 ttgtatcatg agtataataa ctggcctggc ggtgtcaagt aggggccggc ctacctcaag
     4501 gcatttcagt tgtcagcaat tgcccgtagc acagcctgtc aggctgtctt caccggccac
     4561 agcctgacag gctgtgctat gagttgagcg acgggccggg caacaaacga ccggcgacaa
     4621 cctgtaacac agcctgtcag gctgtcttca caggccacag cctaacaggc tgtgctacga
     4681 gttgagcgac gggctgggca acaaacgacc ggcgacaacc tgtaacacag cctgtcaggc
     4741 tgtcttggct gtcttcacag gccacagcct aacaggctgt gctacgggtt gggcgactac
     4801 tgaaaagccc tgagtgaccc caaacgggag tttacagaat gcaccaaact aagtataatg
     4861 acgtaagtgc aggaaggtga ggagccatcg caggaaaggc gggagccaca tggacgcccc
     4921 attgccagac acatctgtcc agccggccga gccagcggtc gagccttcgc gcgacccacg
     4981 gccaccaccc ggctccctga ccgcggacac gccctttgaa gctgctcgtc gtctggaacg
     5041 ccgccaacag gtcgccctga ccatcctttc ggctgtcgcc ccggcgacgg ccatcaacat
     5101 cgctgtcggc tacattgagg aggccatcga ctgcctggcc gccggcattt ccctctacag
     5161 tgccttcccc gaaacgatga ccgtcttgca ggatagcggc gacggctggg agccgggcac
     5221 gcaagtgccc gtgacccgca ccgacatact ggccactgtc gccgcgggcg acctttacta
     5281 tctggccgac ctccgcgctg ccagcgctgg ctcgcccggc ttccagcacg cgctggccat
     5341 gggcggccgc tcgttgctgg cggccccggt acgcgccgac gggcaattgc tgggcgtcct
     5401 gtggatcatc acgggcgacc cacgcccgtt ttcgtcggat gaagagactg tggcccgcga
     5461 agtggccaat gtggtcgcgg tggccctcca caaccgccgc ctactggccg ccgagcagaa
     5521 ggcccgcgcc cgcgagcgca gcctacgcga ggtcggtgcc agcttgacgc tgggtctcag
     5581 ccgcggcacg gtgctcaagc gcattctgga gcaactgggc gagttgatcc cctttgccag
     5641 cgcatccatc gtcttgctag acggcgggga gatcgtcttt gccgcggcgc acgagctggc
     5701 cttgcggccg gaacaggtgg acgacctacg cacccacacg ccgcccatcc tgcgcgcgct
     5761 catagacacg cgcgcccccc ggatgatcga cgacaccgcc acagacccgg actggctgcc
     5821 gctgcccggc ttcagttaca tccgcgcctg gctgggcgtg ccgttgctgt tcaatggcgt
     5881 ctgcatcggc gcgctgacca tcgaccggga gcagccgcgc agcttctcgg ccgccgacgt
     5941 ggaactggcc agcgccttcg ccaatcaggc tgccgtggcc atcgaaaacg cccggctgtt
     6001 taacgaggcc cagcgctacg ccgaacaact ggaggcgcgc gtccgcgagc gcacgcgcga
     6061 actggaagcc ctctatggca tcagcgcggc gacgatcgag cggccggaga tgggcaacgt
     6121 cctggcgcgc gccctggaac tggttgttga cgccttcgat tgcccggcgg cgaccgtcca
     6181 tctgatccag cctggccagc cagagctacc gctcatcgcc cacctggatc gtagcggcag
     6241 ccacctggcg agcttgctgg gtgcgccgcc ggtggccgcg ctgctgcgcc ggcaggcgct
     6301 cggcggcgcg gtccgcattg ccggagacga actggcgacg gccctggccg cagaccgggc
     6361 atgggccgcg gccagcgccc cgttgcgcgc ccacggccgc aacctcggcg tcctgagcct
     6421 gtggagcgac agccccgacg ccttcgaggg gtcgatgcgc ttcctaacag ccgtcgccga
     6481 ccagctcggc gcggcgctgg agaatctgca actgcgccag atgacacgcc aggcggctgt
     6541 tctggaagaa cgcgagcgca tggcgcgcga gctacacgat gccgtcaccc agaccatcta
     6601 cagcgcgggc ctcctggccg aggctgcccg cgaatcagcc cacgccggtg acctgcgggc
     6661 cgtcgaaagc cacacccaga tcattgtgca gcgcgtctac caggcattga gggaaatgcg
     6721 cctactgttg ttcgagctgc gcgccgatac agtggcctgg caggggttgg ccatcgcgtt
     6781 acgcacccgg ctgcaagcgg tcgaggagcg ggccaacatc gccacgcaat tgagcgtcaa
     6841 ggacgtgagc gagttgccgc cagcgctgga agaggccttc tatcgcgtcg ccctggaggc
     6901 gctcaacaac gccctgcacc acgcccgcgc cgggcgagtc agtgtgacgc tgcgcgtcgc
     6961 cgggggcgaa ctgatcctcg tcgtccgtga cgatggcgtg ggcttccgcc ggcgcgatgc
     7021 cgccgccggc agcggcatgg ggctggagag tatggccagg cggttggaca aagtcggtgg
     7081 ccggttgagc atcaccagcc gccaggggcg gggcacgcgc gtcgaagcgc gcgccccgct
     7141 gacggccggg ccacaggagg cagagcagca atgaccgacg ctatccacat cctggttgtt
     7201 gaagaccaga ccatcgtgcg cgaagggcta tgcgcgatgt tggccacgaa gccgggcctg
     7261 cgcgtcattg gcacggccgc cgacggcgag gaggccgtgc atcgcgccct ggcgctgcgg
     7321 cccgatgtga tcctgatgga cctgatcatg ccccggatgg acggcatccg cgccacgcgg
     7381 gagatcgtcg cccaatggcc ggcggcgcgc atcctcattc tgaccagcta cagcgacgac
     7441 gcgcagatct tcgaggcctt tcacgccggg gcgttgggct ttgtgctgaa gcaagacgtg
     7501 ggccagaagc tggtcgaggc tctacaacag atccacgccg ggcaatcgcc gctcaattcg
     7561 acggtgacgc tgcggctgat gcagtcgctg gccatacgca acacggccac gccacgcgaa
     7621 gcgacgctgg agacgcagct caccgagcgc gaattggaga tcgcccgttt ggtcgcccgc
     7681 ggctatgaca accagaacat cgccgacgcg ctgaacatca gcgttcgcac ggtaggcacg
     7741 cacgtgtcca acatcctgcg caaggcaggt gtgagcaacc gcacccaact ggcgcgcctg
     7801 atgctgcggc aagggttggc gtcgctgtac gaatgagcgg cgacgcgcca gacccctcag
     7861 gtctggcgcg tcgcccggcc tactcctccg tgtaatagcc catgcccaga atgcccggcc
     7921 caacgttggc gccgatgacg ggcgtcagtt cattgagcag cagttcatcc ggctgaacgg
     7981 cggccaagac ctgttccttg atagcgaggg cgtcgctgta agcgccggcg tggatgatgc
     8041 cggcgtggat gtgcttcttg cccttcattt cgcccagaac gacctcaagc atgtgggcaa
     8101 cggccttgct cttggtgcgc acagacgcca gcggctcgat ctggccattt tcgatgtgga
     8161 ggacgggctt gatcgacagc accgacccaa ccaggcgctt ggccccgccg atccgcccgc
     8221 ccttgtggag atactccagc gtatcgacga cgaagagcac gcgcatccgc ggcacgaggc
     8281 cacgcacata ctcggccacg gcgtgggtgt cgtggcccgc ggcggcatgg cgggcggcgg
     8341 ccacggccaa caaccccagc cccaacgacg ccgagcgcga atcgacgatc tcgatggggt
     8401 agtcgcccat cgcctgcgcc cccagatggg ccgactggat cgtgccgctg agcgccgaag
     8461 aaacaaacac ggcgacgatg ctgtccgcgc ccgcggcaac cttcatgaag tgctcggtga
     8521 attgtcccgg cgacggttgc gaggttttgg gcaggtcttt cgacttcggc agccgctcgt
     8581 agaactcggt cgtggtgatg tcgatctggt ctagaaggct attgccctcc caattgagga
     8641 tttgggggat gacgtagatg tcatgctcgg cgagcgtctc cggcgtcagg ttggcgacgc
     8701 tatcggtgat aatggcaacc ttcttcttgc tcataaggac tcctgattct ccttccggtt
     8761 aatcgttcgc ttttaggcag cgcggggccg gtcaaacgta aatgatgtgc taggaaaaga
     8821 aacactggac aaggccaata gaatcggtta tcgtatagtg tctctgtgag gttctcccgc
     8881 cactgcggaa gtcaacccgt tcagggttct cccccaccac aaatacgcac aattttgtag
     8941 acaaatatgg cctatttcct gatgtgtcta tcggcaactt cggctacatt tgtcttgtac
     9001 acttcatggg gtgaattgta cgcggggtga attcggtcgc acaagaaacc cggctcattg
     9061 gccgggtttt ttggtgtttg cgtgggttta tcgtcctacc gctcactgta accgtctgca
     9121 tcaggcgcgc ccggcggcga caaattgccg ggttgtggcg acgacatcct tgctgcgcgc
     9181 cagtgcctcg ccgatcagca cggccgccgc gcccgcaacc cgcatggcgg ttacgtcgtc
     9241 aggcgtcttc aggccgctct cggccaccac ggcgacggcc ggcgggatga gcgcccgcag
     9301 tcgcgctgtc gtgcccagat cgatcgcgaa cgtctgcaag tcgcggttat tgacgcccac
     9361 caattccggc cccagcggca agacgcgcgc caactcggcc tcgttgtgca cttcgaccag
     9421 cgcggccatg cccagctgcc gcgccagagc gtgcaactcg gccaggtcgt ggtcgcccag
     9481 tacggcggcg atgagcagga cggcgtcggc cccggccacg cgcgcctcgt agagctgata
     9541 gggatgaaag aggaaatcct tgcgcagcac gggcaggcgc acggcctcgc gcacgtcgcg
     9601 cagatgctcc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccttgcgc
     9721 agcacgggca ggcgcacggc ctcgcgcacg tcgcgcagat gctccagggc accctgaaaa
     9781 tggcgcgcgt cggttagcac agagacagcc cgcgcgccgc ccttttcgta ggcccgcgcc
     9841 agccgcacgg gatcatagtc gggcacgatc agccccttgc tgggcgaagc cttcttgcac
     9901 tcggcgatta gcgccacgcc cggggcgcgc aaggcggccg cgaaatcgcg cggcggcggg
     9961 gcgacggcgg ccatagcccg caggtcagcc agcggccgct cggccatcac ccgcggcagc
    10021 cgctcgcggt ggtgggccat gatctcatcc agaatttcgc cgcggcgctt ggcaagatta
    10081 gttacgctca tcgggcggga ttatagcacg ggcaggaacg gcacgcagat gacgctgatt
    10141 agcggcaggg ctttgccaaa gtcaccccag gcgcatagtc cctggacgac tttggaaaag
    10201 ccctggatta gctgattttc gcagagaagg aattctgatt cttatcggcg taaatcctcg
    10261 tcatcggcgg gccactcggc caacgccgcg gcaatgatct gccgcaggat gccggcgaca
    10321 gcctggggct ggtcaacttc catcaggtgg gtgctgtcga ggtcgtagtg gcgcacgcga
    10381 tggccgtctc gccgcgctgt ggcgatgaga tcgtgtgtcg cctgccagtt gaccatgcca
    10441 tcctgccgcg acagcaggac atgggcatcg gccagcgtgt cggggccgca gacgtcgtcc
    10501 ggctccagcc gcagcaactc ccgcccctgc tccaggagga tgaacgggtt tagccgccca
    10561 tagccgccga agccgcgcag cccgccgatg gtgaagcggt cggccccggc caggtagaac
    10621 agcggcgcga aggcgcgaaa gaggccggtg gccacgggcg cgtagagcgg cagccggcca
    10681 agggtcagct tcatggcgtg gagcacatcc agccggctca gggcggcccg cgcctccggt
    10741 tggaggcaga aggccggggc cgacaggacg cgcgtcggcc gcagccggcg gccggcggcg
    10801 gccatctcct caaccagcgc ctcggtctgg cactggctga gggccagacc gcccatcgag
    10861 tggccatagg cgatccagat gatgtcggtc tctctggcca gcggcccgtc gagcagccag
    10921 cgcaggcccg cgcgcatggc ctgcggctgg tggcggatgc tgtaggcttc caggtggtag
    10981 cggtcgatgg cggcgctggc gggaacgtcg tagccggggt ggttgacggt gaagacgcgc
    11041 agccggaacg ccgcgccg
//
LOCUS       DAOSMQ010000035        11044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_5899_length_11044_cov_4.773501, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000035 DAOSMQ010000000
VERSION     DAOSMQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_11044_cov_4.773501"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PK829_02140"
     CDS             <1..690
                     /locus_tag="PK829_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOU40045.1"
                     /translation="GGNGDGEAASPYSFELCGGLHVSETGEIGLFRFVSESAVGANTR
                     RVEAVTGRGAYEFVSKRLAALDRLAARLNTPVGELESRVEALLEHDRAAERDLETAKR
                     RLLRGDFEVLLGGVLETKGTHLLAAQVKAGDTDQLREMADWYRDRFQSGVVVLGAVKD
                     GKPVIVAAVTDDLIARGLKAGDLVREVAKVVGGGGGGRADLATAGGRNPELLSAALDA
                     VRGHVEATLKD"
     gene            690..1778
                     /locus_tag="PK829_02145"
     CDS             690..1778
                     /locus_tag="PK829_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40046.1"
                     /translation="MRVIELDTAETYYSLRDRILRGPRERIALVVPPRAAVMEGVGLP
                     LLRRLADRERLEIGLVTADGELARRARRAGLPAFASLGLAQHYRPGWWRGRRRAEWLG
                     LPVGGAPPPAATGRAAVPLAIALILLALALTAAAVVYTLPRATVTLRPATQPAQAIVE
                     LADVPARPVALSQSWEATAAGTGAAARQSARALALQALNAAAPAVLAARLAPGEQLVP
                     GATRVTLAGEEYTTARGQTRLALTATLEGTAVDTAEVNRRVLPELARALPAGYAVDPA
                     TLVTRLEPGSAAGRLVVTATATGRAVIDANALAAQLAGQRTVDVGPALAALPLAEPAR
                     LDLSLGPLSTWLPRLPFRPAQIQVEVLP"
     gene            1920..2375
                     /gene="ruvX"
                     /locus_tag="PK829_02150"
     CDS             1920..2375
                     /gene="ruvX"
                     /locus_tag="PK829_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOU40047.1"
                     /translation="MPNDTPSPGRVMALDLGEKRIGIALSDPTRIIASAHSVLARRSR
                     LEDTAHYGRLIAENGVTLLVVGLPMTLGGREGERAGWVRDYAADLAERLPVPIVFWDE
                     SLSTVEAEAALRAQGRRGRKLRARIDAVAAAVILQSYLDAQRGATPDDV"
     gene            2365..3534
                     /gene="mltG"
                     /locus_tag="PK829_02155"
     CDS             2365..3534
                     /gene="mltG"
                     /locus_tag="PK829_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOU40048.1"
                     /translation="MTSEARPEKRSGSCLLRLAVAVVVLVAVGATAVVLLRGPLSADA
                     DVNPALDPIRRTYLKLYLSEHAEALAGPAGRGTTPVVFTVAPGEGATAIAANLRAAGL
                     LDNPELFLNYLTYYGLDGGLVTGEYQLDPQATIPELAEALGSGRSQTLELRFLPGWRS
                     EEMANYLEVVAPAQIDAVAFREVVSSRRGLSGSYSFLTSLPEGASLEGYLFPDAYTVA
                     PDATAAALVDSMLARFDATMTPAMRQSYGTLGLSLRDAVVLASIIEKEAVLDEEKPLM
                     AAVFLNRLAAGMPLQADPTVQYALGYQAATGGWWKAPLSAEDLAVASPYNTYLAPGLP
                     PGPISNPGLASLAAVANPAAVDYLFFVVDCAAATPGAHAFSVTYDEHLANIARCQ"
     gene            3497..4141
                     /locus_tag="PK829_02160"
     CDS             3497..4141
                     /locus_tag="PK829_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU40049.1"
                     /translation="MTSIWRISRAASRLLALALATALLAGCASVGPVVKIGLAAPFEG
                     RDRAVGYDAIYAARLAVREVNARGGIGGTRVALVALDDRGDPVLATQTAASLVVDPGV
                     VAVVGHYLPETTTAAADIYAKAGLPLLAAGAPPFAAANPAALPPAFVAAYEKVTPFDE
                     TPGPYAGPTYDAFGLLWAALAQAEERGGELSRASVQQALRGLEYRGVTGTVYQP"
     gene            4164..5189
                     /locus_tag="PK829_02165"
     CDS             4164..5189
                     /locus_tag="PK829_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40050.1"
                     /translation="MVIRRILGLIILLTALVIAAGSIYAAVVVGDALASLGAGIRDNL
                     ALASNTLETARNTLALSRDTFDDVNGGLGTAVQATANASRTIGDSQPLIDNVAVVTTQ
                     EVPEAVEGIQNALPNMIEVAGVIDTTLRTLSNIGIDRTIDLPFGGSIPLQFDLGIDYA
                     PATPFDVSLRSFQASLEGLPESMRGLEDELQLTSDNLGALATDLQATSDNLAVINGRV
                     GEVLPLLDQYLILADQLSATVTRVDADIDRQLETLRLGAIGVLGLLALSQLAPLVLGW
                     ELLTGRRGPARPVEVIRETIIAPAPAALPPDTVPLTAVLPMPAGTVEMPAEEWSPLAP
                     PEDDDSV"
     gene            5176..7830
                     /locus_tag="PK829_02170"
     CDS             5176..7830
                     /locus_tag="PK829_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU40051.1"
                     /translation="MIAFRRGLARARWHTLWLVAVLVGAVACRGTEAVETPTGRAQPV
                     TGANAALLPTPTLAAGVLAATVDPLRPPTPIVLPSATPLGAPEGQPDEAILAAGTPAA
                     STADTAEVAGETIPPQPTVALPPGATAPELLALGRQSAAEGDNATAAAAFRAAAEAGE
                     LSAREAAEAQLGHAMALFDDGQYQAAEEALAALLQDPAAAAAAPARPATTALVTPIDI
                     PDVAAFTLGRARAALGNHAGAIEAYRAYAQNNPDMAAYVQPRIADAHLALGDTAAAIA
                     ALELAAQGAAQRFVAVENRGRLAQLYLDRGDTAAAVAQYDAIRDIARTEATKGQMTYL
                     AGQAEERAGHVAAAQERYRDAVANYPRAADSYAALVALVDAGAPVDEYQRGVVDFYAG
                     AYDVGIEALRRAIAANAEGYPQDAHLFLAWSYEGLGDLTSALVELDAYAAHDAARALF
                     ERGEMLARAGRANEALAAYDAFLSDYPDAAEAPAVHWTAAQLADSAGATDAAGRYLAL
                     ADAFPFDDETPAALYRAGELTAAGDPAAGAALWARLAEQYPANEFGAQALFRLLQLAG
                     VGEVEGLDAGALRAQVDTLTPSNYYALRARDVVAGREPFTADAPLTLADTAGQAEAET
                     WLRQRLVAAGVTPPEMLGALSAGLAAEPGRRIGEKLWQLGLHDAAKAELETVREAYAG
                     DAVANYQMALYFDELGLYRSSIIAAVSLLNQVGATIYDAPRFLGRLSFPIHYADLILP
                     LAERYNFDPRLQFALVRQESLFESIARSGAAAQGLSQVIPDTGAWIAQRLAWPNYDND
                     DLFKPYVGLNFGAYYLSEQLRTFDGHVFAALAAYNGGPGNAARWYEAAGADHDRFVDT
                     VDFSETRLYIQRIYEGFSAYRALYGGLN"
     gene            7867..10458
                     /locus_tag="PK829_02175"
     CDS             7867..10458
                     /locus_tag="PK829_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40052.1"
                     /translation="MSFDWQTEDRDWEEQEPRRAGRWALDTPAGEDTAAAPERAETLA
                     SSRPRHRWLFVAAVGGLVVALLAVLYWQLERRSTAAQTRLATEVIASHATILDAAQRG
                     DGELFVSFLSGRDAAWSRAQERLVRAGGFVDRTAFEMHWTPAAPVSPTVTLAPDLRSA
                     ELIAPQAYAVEIGNGLTETIVLQQTAIYRQGPDRWLYSPPEEDYWGQTEAMGNRYVRV
                     HYPARDADIVEPLARDMDATLAELCRLVGGLCAGNIFIDLTFSTDPAALSEYRTPQDT
                     WAGGGKLALPTPTLFGRPADAAAYRALSRAYTSRVVSAAAANLAGWACCDDQLFYGAL
                     LDAQLGQLGLRPWPLEAAAHETLVGRPELLRDVEGLWPGGDATPEQRLAVYALVDFLV
                     ARNDAPIVEMQRLLLDESTGDYWSWLARITNGAFASPADFERALLSYSAERRRATAPL
                     PLPAQALQLVCRQADAAWPALYRYDPVDGQLTYEQELTGFEEPMIVALPGRDGIAVFA
                     REREQQFPMTYLWHDGAITTVYPGATDGNPRLVALPDAPKDDALLVVQEGAPSVYALL
                     LLDGCLQPQATCITTPPIGPITYSPDATRSLLVAGEPSPFSDQVGAPIIYLGDAEGQV
                     HSSQAFVEAGISPFWLSDDTFGYIATRQDGSGQERVVLRTIASSPGNTRLTAAADGSL
                     FSVRDLEPLVGSRGEGGLTIDRVLPNPAADELLVFTAGPSNPEQANLALTYDLTTGEL
                     RPRFALADEAFDYRRAYRRAYRFSPDGRWLAVSTLAWSATPGVQGTVDWWVYVHATDG
                     SLTRRYALRAGDTWPAHWLSDWSADGRWLALATGGYVRLVAPQEDYSLPIILPDKACS
                     AAVWAKG"
     gene            10472..>11044
                     /locus_tag="PK829_02180"
     CDS             10472..>11044
                     /locus_tag="PK829_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40053.1"
                     /translation="MRSNFDWQTEDDDRREHGGWDEPAEPRPAAPRRRPPWRALGLIG
                     GLLLAVGALIWWRVDKRIEATTQAMRTDVVASHNLVQRAVADGDTEIFRASLSGRDPA
                     WTEGTLAVFESGLFADRAPFGLSPVEGSLPVMLPAPDEEATADQRPAVIALSADLNEA
                     IVTVDQPYRRDDGGTILLQQTTIFRRGDRRW"
BASE COUNT         1575 a         4052 c         3764 g         1653 t
ORIGIN      
        1 ggcggcaacg gcgacggcga ggctgcgtca ccctatagct tcgagttgtg cggcggcctg
       61 cacgtcagcg agacgggcga gatcggcctg ttccgcttcg tcagcgaatc ggccgttggc
      121 gcgaacaccc gccgcgtgga ggcggtcact ggccgcggcg cgtatgagtt cgtcagcaag
      181 cggctggcgg cgctcgaccg tctggcggcc aggctcaaca cgcccgtcgg cgagctggag
      241 agccgcgtcg aggcactgct ggagcacgac cgcgccgccg agcgcgacct ggagacggcc
      301 aagcggcggc tgttgcgcgg cgacttcgag gtgctgctcg gcggcgtgct ggagacgaag
      361 ggcacgcacc tgctggccgc ccaggtcaag gccggcgaca ccgaccagtt gcgcgagatg
      421 gccgactggt accgcgaccg ctttcagtcg ggcgtggttg tcctcggcgc ggtcaaggac
      481 ggcaagcccg tcatcgtcgc cgccgtgacc gacgacctca tcgcccgcgg cctgaaggcg
      541 ggcgacctgg tgcgcgaagt ggccaaagtc gtgggtggcg gcggcggcgg gcgggccgac
      601 ctggccacgg ccggcgggcg caacccggaa ttgctgtcgg ccgccctcga cgccgtgcgc
      661 ggccacgtgg aggcgacgct caaggactga tgcgcgtcat cgagctggac acggccgaga
      721 cttactattc gctgcgcgac cgcatcctgc gcggcccgcg ggagcgcatc gctctggtcg
      781 tgcctccgcg ggcggcggtc atggagggcg tcggcctgcc tctgctgcgg cgcttggccg
      841 accgcgagcg gctggagatc ggcctggtga cggccgacgg cgagctggcc cggcgggcgc
      901 ggcgcgccgg gctgccggcg ttcgcctcgc tgggactggc ccagcactac cggccgggct
      961 ggtggcgggg gcggcgccgt gccgagtggc tgggcttgcc cgtcggaggc gcgcctccgc
     1021 ccgcggcgac cggccgggcg gccgtacccc tggccattgc cctcattctg ctcgcgctgg
     1081 ccctgacggc cgccgcggtg gtctacacct tgccgcgggc gacggtgacg ctgcggccgg
     1141 cgacacaacc ggcgcaggcc atcgtcgaac tggccgatgt gccggcgcgg ccggtggcgc
     1201 tgagccagtc atgggaggcg acggccgcgg gcacgggcgc ggccgcccgg cagagcgccc
     1261 gcgccctggc cctccaagcc ctgaatgccg ccgcgccggc ggtgctggcg gcgcggctgg
     1321 cccccggcga gcagctcgtg cccggcgcga cgcgcgtcac tctcgccgga gaggagtaca
     1381 cgaccgcgcg cggccagacg cgcctggcgc tgacggccac gctcgaaggc acggccgtgg
     1441 ataccgccga ggtgaaccgg cgcgtcctgc cggaattggc ccgcgccctg ccggccggct
     1501 atgccgttga cccggccacg ctcgtcacgc ggctggagcc gggcagtgcc gccggccggc
     1561 tggtggtgac ggcgacggca accggccggg cggtcatcga cgccaacgcg ctggcggcgc
     1621 aattggccgg acagcgcacc gtcgatgtcg ggccggcgct ggccgcgctg ccgctggccg
     1681 agccggcgcg gcttgacctg tcgctggggc cactgagcac gtggctgccg cgcctgccct
     1741 tccgcccggc tcagattcag gtggaagtgc tgccttagct ccatgcgggc cgatcccgtt
     1801 ggccaaacgt cgggcaacaa ttgggacgcc gccatcgtct agagagataa tagcgggcaa
     1861 tctacgggcc gcgaaggacg gcagcccgca aacagacgat aaacagcagc cactgccata
     1921 tgcccaacga caccccatcc cccggccggg tcatggccct tgacctgggc gagaagcgca
     1981 tcggcatcgc cctgagcgac ccgacccgca tcatcgcctc ggcccacagc gtcctcgccc
     2041 gccgctcgcg cctggaggac accgcccact acggccgcct catcgccgag aacggcgtca
     2101 ctttgctcgt cgtcggtctg cccatgaccc tcggcgggcg cgaaggggag cgagccggct
     2161 gggtgcgcga ctacgccgcc gacctggccg agcgcctgcc cgtgcccatt gtcttctggg
     2221 acgagagcct gagcacggtg gaggccgagg ccgccctgcg cgcccagggg cggcgcgggc
     2281 gcaagctgcg cgcgcgcatc gacgccgtgg ccgcggccgt catcttgcag tcctacctgg
     2341 atgcccagcg cggagcgacg cccgatgacg tctgaggcgc ggccggagaa gcgctccggc
     2401 agctgcctgc tgcggctggc cgtggctgtg gttgtcctgg tggccgtggg cgcgacggcg
     2461 gtcgtgctgt tgcgagggcc gttgagcgcc gatgccgacg tcaaccccgc ccttgacccc
     2521 attcgccgaa cgtacttaaa gctatacctg tctgagcacg ccgaggcgct ggctgggccg
     2581 gccggccgcg gcacgacgcc agtggtcttc accgtcgctc ccggcgaagg agccacggcc
     2641 atcgccgcga acttgcgcgc tgccggcctg ctggataacc cggagttatt tctcaactac
     2701 ctgacctatt acggccttga cggcggcctg gtgacgggcg agtaccagct cgacccgcag
     2761 gccaccatcc ccgaactggc cgaggcgctt ggctccggcc gctcgcagac gctggagctg
     2821 cgcttcctgc ccggctggcg cagcgaggag atggccaact atctggaggt cgtcgccccg
     2881 gcacagatcg acgctgtcgc cttccgcgag gtggtcagtt cgcggcgcgg gttgagcggc
     2941 agctacagct tcctgaccag cctgccggag ggcgcgtcgc tggagggcta cctcttcccc
     3001 gacgcctata ccgtcgcccc ggacgccacg gccgcggccc tggtagacag catgctggcc
     3061 cgcttcgacg ccacaatgac gccggccatg cgtcagtctt atggcacgct gggcctgtcg
     3121 ctgcgcgacg ccgtggtttt ggcctcgatc atcgagaagg aggctgtgct ggacgaggag
     3181 aagccgctca tggccgccgt cttcctcaac cggctggccg cgggcatgcc cctgcaagcg
     3241 gacccgacgg tgcagtacgc tctcggctat caggcggcga cgggcggctg gtggaaagcg
     3301 ccgttgtcgg ccgaagacct ggctgtggcc tcgccctaca acacctacct ggctcctggc
     3361 ctgccccccg gcccgatcag caaccccggc ctggcctcgc tggccgccgt ggccaacccg
     3421 gccgcggttg attacctgtt cttcgtcgtc gattgcgccg cggctacgcc cggcgcacat
     3481 gccttcagtg tgacctatga cgagcatctg gcgaatatcg cgcgctgcca gtaggttgct
     3541 ggctctggcg ctggccacgg cgctgctggc cggctgcgcc tcggtcgggc cggtggtcaa
     3601 gatcggcctg gcagccccct tcgagggccg cgaccgggcc gtgggctacg acgccatcta
     3661 cgccgcgcgg ctggccgtgc gcgaggtcaa cgctcgcggc ggcattggcg gcacgcgcgt
     3721 ggctctggtg gccctggacg accgcggcga cccggtgctg gcgacgcaga cggccgcctc
     3781 gctggtcgtc gatcccggcg ttgtcgccgt cgtcggccac tatctgccgg agacgaccac
     3841 agcagctgcg gacatctatg cgaaggcagg gctgcccctg ctcgccgctg gcgcgccgcc
     3901 cttcgccgcc gcgaacccgg ccgccctgcc gcccgccttt gtagccgcct atgagaaggt
     3961 tacccccttc gacgagacgc ccggccctta tgccgggccg acctatgacg cctttggcct
     4021 cctttgggcc gccttagcgc aggctgagga aagagggggg gaactgtctc gcgcctctgt
     4081 ccaacaggcg ctgcgtggat tagaataccg gggcgtgaca ggaaccgtct accagccctg
     4141 atccgcccca cggagcgagc cgcatggtca tcagacgaat tctcggcctc atcatcctcc
     4201 tgaccgctct ggtcatcgcc gccggcagca tctatgctgc cgtggtcgtc ggcgacgctc
     4261 tggccagtct gggcgctggc atccgcgata acctcgctct ggcctccaac actctggaga
     4321 cggcccgtaa caccctggcc ctatcgcgcg acaccttcga cgacgtgaat ggcgggctgg
     4381 gcacggctgt acaggccacg gccaacgcct cgcgcaccat cggcgacagc cagccgctga
     4441 tcgacaacgt ggcggttgtg acgacgcagg aagtgccgga ggctgttgag ggcatccaga
     4501 acgcgctgcc caacatgatc gaagtggccg gcgtcatcga cacaaccctg cgcacgctca
     4561 gtaacatcgg catcgatcgc actatcgacc tgcccttcgg cggctcgatt cccctccagt
     4621 tcgatctggg catcgactac gccccggcga cgcccttcga cgtcagcctg cgcagcttcc
     4681 aggccagcct tgagggcttg ccggagagca tgcgcgggct ggaggacgaa cttcaactga
     4741 ccagcgacaa cctgggcgcg ctggcgaccg atctacaggc cacctccgac aacctggcag
     4801 tcattaacgg ccgcgtcggc gaagtgttgc cactgctcga tcaatacctg atcctggccg
     4861 accagttgag cgccaccgtg acgcgcgtcg acgccgacat cgaccggcaa ctggagacgc
     4921 tgcgcctggg ggcgattggc gtgctgggcc tgctggccct gtcgcaactg gccccgctgg
     4981 tgctcggctg ggagctattg accggccggc gcggcccggc ccggcccgta gaggttatcc
     5041 gcgagaccat cattgctcca gcgcccgcag cgctgccgcc cgatacggtt ccgctcacgg
     5101 ccgttctgcc catgccggcc ggcacagttg agatgcctgc ggaggagtgg tcgcccctgg
     5161 ccccaccgga ggacgatgat agcgtttaga cgcggcctcg ctcgcgcccg atggcacacg
     5221 ctgtggctcg tggctgtcct cgttggggca gttgcctgcc ggggcacgga ggctgtggag
     5281 acgccaaccg ggcgggcgca acccgtaacc ggggccaacg ccgcgctact gcctacgccg
     5341 acactggccg ccggcgtcct cgccgccaca gtcgaccccc tccgcccgcc aacgcccatc
     5401 gtcctgccat cggccacgcc gctgggcgcg ccggaaggcc agcccgatga ggccattctg
     5461 gccgccggca caccggcggc cagcaccgct gacacggccg aggtcgccgg cgagacaatc
     5521 ccgccacagc cgacggtcgc cctgcccccg ggcgccaccg cgccggagtt gctggcgctg
     5581 ggccggcaat cggccgccga aggggataat gccaccgccg ccgcggcctt ccgcgccgcc
     5641 gccgaagccg gcgaactgag cgcccgtgag gccgccgagg cgcaactggg ccatgccatg
     5701 gccctgttcg acgatggcca gtaccaggcc gccgaagagg cgctggccgc cttgctgcaa
     5761 gaccccgccg ctgccgccgc cgccccggcc cggccggcga cgaccgcgct ggtcacgccc
     5821 atagacattc ccgacgtagc tgccttcacc ctgggccggg cgcgggccgc gctgggcaac
     5881 catgccggcg cgatcgaggc ttaccgcgcc tatgcccaga acaaccccga catggccgcc
     5941 tacgtccaac cgcgcatcgc cgacgcccac ctggccctgg gcgatacggc cgcggccatc
     6001 gccgcgctgg aattggccgc ccagggcgcg gcgcagcgct tcgtggccgt ggagaaccgt
     6061 ggccggctgg cccagctcta cctggacagg ggcgacacgg ccgcggctgt ggcccaatac
     6121 gacgccatcc gcgacatcgc ccgcaccgag gccaccaagg ggcagatgac ctatctggcc
     6181 gggcaggccg aggagcgcgc cggccacgtc gccgccgccc aggagcgcta tcgcgacgcc
     6241 gtggccaact acccgcgcgc cgccgacagc tacgccgccc ttgtggccct ggtcgacgcc
     6301 ggcgcgcccg tggacgagta ccagcgcggc gtggtcgact tctacgccgg ggcctacgac
     6361 gttggcatcg aggcgctgcg ccgggctatc gccgccaacg ccgagggtta tccccaggac
     6421 gcccacctct ttctggcctg gagctacgag gggctgggcg acctgaccag cgccctggtc
     6481 gaactggacg cctacgccgc ccacgacgcg gcgcgggcgc tgttcgagcg cggcgagatg
     6541 ctggcccgcg ccggacgggc gaacgaggcg ctggcggcct atgacgcttt cctgagcgac
     6601 taccccgacg cggccgaggc cccggctgtc cactggacgg cggcgcaact ggccgacagc
     6661 gccggggcga cagacgcggc cggccgctac ctggctctgg ccgacgcctt cccctttgac
     6721 gacgagacac ccgcggccct ctaccgcgcc ggcgaattga ccgccgcagg cgacccggcc
     6781 gccggcgcgg ccctgtgggc gcggctggcg gagcagtacc cggccaacga gtttggcgcg
     6841 caggcgctct tccggctgct gcaactggcc ggcgtgggcg aggttgaggg gctggacgcc
     6901 ggcgcgctgc gggcgcaggt cgatacgctg acacccagca actactacgc cctgcgtgcc
     6961 cgcgatgttg tcgccggccg ggagccgttc acggccgacg cccccctgac gttggcggat
     7021 actgccgggc aggccgaggc cgagacctgg ctgcgccaac ggctggtggc cgccggcgtc
     7081 accccaccgg agatgttggg agcgctctcg gctgggctgg ccgccgagcc gggccggcgc
     7141 atcggcgaga agctctggca gctgggtctg cacgacgcgg ccaaggcgga actggaaacg
     7201 gtgcgcgagg cctacgccgg cgacgccgtg gccaactacc agatggccct ctactttgac
     7261 gagttgggcc tctaccgctc gtcaatcatt gccgccgtgt cgctgctcaa ccaggtcggc
     7321 gcgaccatct acgacgcgcc gcggttcctg ggccgcctgt cctttcccat ccactacgcc
     7381 gatctcatcc tgccgctggc cgagcgctac aacttcgacc cgcgcctcca gttcgctctc
     7441 gtgcgccagg agagcctctt cgagagcatt gcccgcagcg gcgcggcggc gcagggtctc
     7501 agccaggtca tccccgacac cggcgcatgg atcgcccaac ggttggcctg gcccaactac
     7561 gacaacgacg acctgttcaa gccctacgtc ggcctgaact tcggcgcgta ctacctcagc
     7621 gagcaactac gcaccttcga cggccacgtc ttcgccgcgc tggcggccta caacggcggg
     7681 ccgggcaacg ccgcccgctg gtatgaggcc gccggggccg accacgaccg cttcgtggac
     7741 accgtcgatt tcagcgagac gcgcctctac atccaacgca tctacgaggg cttcagcgcc
     7801 tatcgcgcgc tttatggcgg cttgaattag cccgttcgtg ccgccagccc cggccacagc
     7861 tataccatgt cctttgactg gcagaccgaa gaccgcgatt gggaagaaca agaaccgcgc
     7921 cgcgccggcc ggtgggcgct cgacacgccc gccggcgagg ataccgccgc ggccccggag
     7981 cgagccgaaa cgctggcctc ttcccgaccg cgccaccgct ggctgtttgt cgccgcggtc
     8041 ggggggctgg tggtagctct gctggccgtg ctctactggc aactggagcg gcgcagcacg
     8101 gcggcgcaaa cgcgcctagc gaccgaagtg atcgccagcc acgccaccat tctcgacgcc
     8161 gctcagcgcg gcgatggcga gctattcgtc agcttccttt ctggccgcga cgccgcctgg
     8221 tcacgcgccc aggagcggct ggtacgcgcc ggcggctttg tcgaccgcac ggccttcgag
     8281 atgcactgga cgccggccgc gcccgtctcc ccgacggtga cgttggctcc cgacctgcgt
     8341 tcggccgagt tgattgcgcc gcaagcctat gccgtcgaga tcggcaacgg cctgacagaa
     8401 accattgtgc tccagcagac ggccatctac cgccaggggc cagaccgctg gctctattcg
     8461 ccgcccgagg aggactactg gggtcagacg gaggcgatgg gcaaccgcta cgtccgcgtc
     8521 cactaccccg cccgcgacgc cgacatcgtc gagccgctgg cccgcgatat ggacgcgacg
     8581 ctggccgaac tgtgccggct ggtcggcggc ctctgcgccg gcaacatttt catcgacctg
     8641 accttttcga ccgatccggc cgcgctcagc gaataccgca cgccgcagga cacctgggcc
     8701 ggcggcggca agctggcgct gccaacgccg acgctcttcg gccggccggc cgacgccgcc
     8761 gcctaccgcg ccctcagccg ggcctacacc agccgcgtcg tcagcgccgc ggcggccaat
     8821 ctggccggtt gggcgtgctg cgatgaccaa ctgttctacg gcgcgctgct cgacgcgcaa
     8881 ctcggccagt tggggctgcg gccgtggccg ttggaagcgg cggcgcacga gacgctggtc
     8941 ggccggccag aactgctgcg cgacgtggag gggttgtggc ccggcggtga cgccacgccg
     9001 gagcagcgtc tggccgtcta tgcgctggtc gatttcctcg tcgcccgcaa cgacgcgccc
     9061 atcgtcgaga tgcaacggct gctgctggac gaatccacgg gcgactactg gagttggctg
     9121 gcccgcatca ccaacggggc ctttgcctct ccggccgact tcgagcgtgc cctgctgagc
     9181 tattccgccg agcgacggcg ggcgaccgcg ccgctgccct tgcccgccca ggcgttgcag
     9241 ttggtctgcc gccaggcgga cgcggcatgg cccgctctct atcgttacga tccggtggac
     9301 ggtcagttga cctacgagca ggagttgacc gggttcgagg aaccgatgat cgttgctctg
     9361 ccggggcgag acggcattgc cgtcttcgct cgcgaacgag agcagcagtt tcccatgacc
     9421 tacctctggc acgatggcgc gatcacgacc gtctatcccg gggcgaccga cggcaacccg
     9481 agactcgtcg ctctccccga tgcccccaag gacgacgcgc tgcttgtggt gcaggagggc
     9541 gcgccgtcgg tttatgccct gctgttgctg gatggctgcc ttcagccgca agcgacctgc
     9601 atcaccacgc ctcctattgg ccccatcacc tactcacccg acgccacgcg cagcctgctc
     9661 gtggccggcg aaccgtcgcc cttctcagac caggtcggcg cgccgatcat ctatctcggc
     9721 gacgccgaag gccaggtgca ctcctctcag gcctttgttg aagcgggtat ctcccccttc
     9781 tggctgagcg acgacacctt tggctacatc gccacgcgcc aggacggcag cgggcaggag
     9841 cgggtcgtcc tgcgcacgat cgcgtcttcg cctggcaata cccggctgac ggctgccgcg
     9901 gacggctctc tattctccgt ccgggatctt gagccgctcg tcggtagccg gggcgagggc
     9961 ggcctgacca tcgaccgcgt cttacccaat ccggccgcgg acgagctact ggtcttcacc
    10021 gctgggccga gcaaccctga gcaagcaaac ctggccctga cctacgacct gaccacgggc
    10081 gagctgcgac cacgcttcgc cctcgccgat gaggcgttcg actatcgtcg cgcctatcgt
    10141 cgcgcctatc gtttctcacc cgacggccgt tggctggccg tcagcacgct cgcctggtcg
    10201 gccacacctg gggtgcaggg cacagtcgac tggtgggttt acgtccatgc caccgacggc
    10261 tccctgacgc gccgctatgc gttgcgcgcc ggcgacacgt ggccagccca ctggctgagc
    10321 gattggtcgg ccgacggccg ctggctggcg ctggcaaccg gcggctacgt gcgtctggtt
    10381 gccccccagg aggactacag tctccctatc atcctgcccg acaaggcttg tagcgccgcg
    10441 gtttgggcta agggataagc aaggtgcgtt catgcggtct aacttcgatt ggcaaaccga
    10501 ggacgatgac cggcgcgaac atggcggttg ggacgagccg gccgaaccgc gaccggccgc
    10561 cccccgccgc cggccgccgt ggcgcgcgct gggcctgatc ggcgggctgc tgctggccgt
    10621 cggcgcgctc atctggtggc gcgtcgacaa gcgcatcgag gcgacgacgc aggccatgcg
    10681 caccgatgtc gtcgccagcc acaacctcgt ccagcgggct gtggccgacg gcgacaccga
    10741 gatctttcgc gcctccctgt ctggccgcga tccggcctgg acagagggca cgctggccgt
    10801 cttcgagagc ggcctgtttg ccgaccgcgc tccgttcggg ctgtcgcccg tcgaggggtc
    10861 gctgccggtc atgctgcccg cgcctgacga ggaagcgacc gccgatcagc gcccggcggt
    10921 catcgcgcta tcggccgacc tcaacgaagc tatcgtcact gtcgaccagc cctaccgccg
    10981 cgacgacggc ggcaccatcc tgttgcaaca gacgaccatc ttccggcgcg gcgaccggcg
    11041 ctgg
//
LOCUS       DAOSMQ010000036        10906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6005_length_10906_cov_4.284951, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000036 DAOSMQ010000000
VERSION     DAOSMQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10906
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6005_length_10906_cov_4.284951"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PK829_02185"
     CDS             complement(<1..1255)
                     /locus_tag="PK829_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU40054.1"
                     /translation="MSTEKQMVGEVYYPAPEVVAHAHIRDYEKVYAEAAADLSAFWGR
                     IAAEGFEWYAPWDMVLDDSNAPFYKWFDGAQVNIVHNALDRHLRTSTRNKAALIFEGE
                     PGDTRTFTYQQLSYEVSRFAGVLKSQGVGRGDRVTIYMGRIPELVIAMLACAKIGAIH
                     SVVYGGFSVEALYDRILDSNSKVLVTCDGAWLRGEIVELKNIVNEALQRNNPVQSVIV
                     VKRTGQAVEMVADRDHWYHDLMALPIADPRTPTEHMDAESPLFMLYTSGTTGRPKAIL
                     HTHGGYMVGTATTLKWVFDIKPHDIYWCAADPGWITGHSYIVYAPLVLGATSFMYEGA
                     PTHPNPDRWWSMIDKYRITILYTAPTAIRGLMRFGESWPQLHDLSSLRLLGSVGEPIN
                     PEAWRWYYRVIGKERCPIMDTWWQTE"
     gene            1366..2748
                     /locus_tag="PK829_02190"
     CDS             1366..2748
                     /locus_tag="PK829_02190"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40055.1"
                     /translation="MTPPKIVIIGAGSASFGPTTLATILRDEALRGSELALVDLNARS
                     AENAARVAERMNAAWGAGMTISATTERREALPGATHVVVSIEVAPREALWQLDWEVPL
                     RHGLRQPYAENGGPGGLMHTCRQVPAHLAIARDMEELCPSAWLILFSNPLPRLVRAIT
                     KYSRIRVVGKCHQINVGYGLVAALLRDRYGVDVPNGVTLHSDPGNTGVVHTLATAGRR
                     HVSITSAGLNHFIWLLDIRDRATGEDLYPALRAAVADAPPTLEPLSLDVFRAFGLLPI
                     AGDTHLAEYLPWLHDPVAKPWETYRLPLYDWRGNEGVRDVLQGMMQMLADGVLPVEGM
                     REAPSEGAAELIVGLNGQTYLDETVNVPNCGAIAGLPPETIVEVPAIVGPTGIRPAQL
                     GQLPPPVAELLRREAALVEVVVDAAATGDRDLARQALLLDPMTGDIGRARAILDDYLQ
                     TFAAYLPQFK"
     gene            complement(2760..3269)
                     /locus_tag="PK829_02195"
     CDS             complement(2760..3269)
                     /locus_tag="PK829_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU40056.1"
                     /translation="MESLQSYLTILLGAAGGLLALVWLALVVWGYRDIRARSRSTVAA
                     VLTILLVALLPVVGLVVYLLLRPRETLAAAYDHALEQEALLQQIEDKPVCPTCARPTQ
                     PNWFLCPACHTHLRQPCPVCRSPLELHWDICPYCGYLFPDPAAEPLAEADGATPVPVD
                     GDVSTTSET"
     gene            complement(3380..3592)
                     /gene="rpsU"
                     /locus_tag="PK829_02200"
     CDS             complement(3380..3592)
                     /gene="rpsU"
                     /locus_tag="PK829_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU40057.1"
                     /translation="MNTLPKVTLRPDESGEQLLRRFSREVVKSRVLTDVRRKRWFVSK
                     SELTRIAKKKAARRTRKSQKEQQQGV"
     gene            3885..5639
                     /locus_tag="PK829_02205"
     CDS             3885..5639
                     /locus_tag="PK829_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOU40058.1"
                     /translation="MTWVAAALFLTIAVHLSAQPPPSTALANEPTLVARVYYNDAADL
                     ARLTNYDVWEYNNVAQRYVLAAVAGDDLDALRAAGWRVAVDETATARLADRAAKPMFF
                     DGYRSVDELYAALDAAAAAHPELTELLTYGQSYCLASGGCVTPGGDALAGYPLRAIRV
                     TNEAIAGSSLISGATVTRGEKPVFFLMANIHAREITTPEIALRFLDLLLDGYGTDANV
                     TWLLDWHEIWIVPTANPDGHWLVELGEERAYGGLPFMQRKNARLGNNIDGLDDCDAWP
                     PDGGTQFGVDLNRNHSFAWGGSGSSDEPCDPVFRGRAPASESEVARLEALLKALFADR
                     RGPAMDDPAPADTAGLLITLHSFSNLVLWPWGHLDVAAPNKTDLQAIGDKLATFNGYR
                     SCQAAAYDCLYAAAGATDDWAYGELGIPAFTFEMGDQFMPSYNEVDGVQWPANGPALL
                     YAATIARAPYQLAHGPDARDVLVAGPGPVRSVSATLDATSNSGLPIAAAEYTVDVPPW
                     QEEALPVALTATDHVFNAAVEKVVGEMNTSALPPGGHILYLRGQDSAGNWGPVSAAFI
                     TVDATPDTWLYLPSVLGP"
     gene            complement(5703..7697)
                     /locus_tag="PK829_02210"
     CDS             complement(5703..7697)
                     /locus_tag="PK829_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40059.1"
                     /translation="MGFHGGGWWAFLSQDEEQDRPVVTRDLLGRVWQFARPYNREVVL
                     LLLSILLTTGITLVTPLLMRTLIDDVIPNADTRRLAWLAAALITVPILNGVVGVWQRK
                     LSAFVGESIIFDLRCAVYEHFQRMSLRFFTQSRTGELMSRLNNDVVGAQQAVSNTLIA
                     IFSNIVQVIGTLAIMLALEWRLTLLGLVVLPFFYLPARRIGRVLRDLRRRSMVLNAEM
                     NATMNETLNVSGALLVKLFGREERELARFRRDSAAVRDLGVHSAVIGRWFFMVLGIIG
                     AVGSALVYWVGGYLTIQGAFTIGTIVAFGAYLSQLYGPLMALTNAPVEFAQSMVSFER
                     VFEVLDIPLEIAEEPDAVPLRDVAGQIAFDQVTFDYAASGEKKAALAEVVRYDWGSGE
                     TLLRRGQGRKPRPVAYRPDGEPDGAEVAAANGNGTNGSEPGTLRRVALRDVDFRIEPG
                     QLAALVGPSGAGKTTVTYLIPRLYDPTGGRVLIDGHDLRDVTLNSLAENIGMVTQETY
                     LFYDTIRANLLYARPDATEAEMIAAARAANIHDFIAGLPDGYETVVGERGYRLSGGER
                     QRVAIARVILKNPRILVLDEATSHLDSLSEALIQDALQHVMQGRTSLVIAHRLSTILA
                     ADVILVMDEGRLVERGTHDELLAGGGLYASLYETQFRAQV"
     gene            complement(7727..10111)
                     /locus_tag="PK829_02215"
     CDS             complement(7727..10111)
                     /locus_tag="PK829_02215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU40060.1"
                     /translation="MRQLLSRPACVAVLLLWVMFALALTSMSGEGPTFDEQGFLVRGL
                     GYLRGPEHGGTRTIIVGHPIGLNALNAVLLVADPTVRLPSDDPSWGETSFHRPAELFL
                     WGRGNDVARTMFLARVPTIWLGMLMAAVVGRWAGQMAGGWRLGRSAANGAGLLALALA
                     ALDPNILAHTRLATTDLGLAAGAALAGYTLWRFLRRPSWPAAILAGMGLGLLQNTKFT
                     ALLFVPLFGVVIFLALWTRRRTLHRRLLLQTLLVYPAVALLTLWATSGFQIGPLRAPL
                     PVVGLLGGAPVPLADYFAQLLAIGNRMQVGAPSFLLGRYSDTGWWYYFPVAFALKTPL
                     PTLLLFGLAIFRLASMIPRRRDRFQIAGFDLAALLIPAGGYFAIALTTDINLGYRHLL
                     AVLPFLMVLIGAVVAQTARKRASQAPGLAGRNRTAVVVTAGVAWLALATVWVYPHFIS
                     FFNVLAGGPDNGWRALVDSNIDWGQDLGRLKTWMADRGVARVWLSYFGEGRPEYYGIA
                     YDGLDSFPPRLMNPVARPFYPHDPAPGWYAISATTLQGVHFADHDQFRFFRERPADDK
                     VGYSIFLYRVAPRGEPVDLLLASTQMDSLAAADFAQLGTNQVTPRWFEADRALVLPAG
                     VAPLWLLLPAEEDVPAALRPFLDYGPAAVAAGDGYRLFRAVARPPATEAGLSLNGNAI
                     AYGQDSWARQDGRAITTLTIWRQEGAPRPIQIFIHVLDQSGQLMAQWDGLSAAWEGWR
                     PGDTLVQLATVTLPESAPSGTYRVVAGLYDPLTNQRWRFPDGRDSVELGQFAIP"
     gene            complement(10194..>10906)
                     /locus_tag="PK829_02220"
     CDS             complement(10194..>10906)
                     /locus_tag="PK829_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40061.1"
                     /translation="NVGQIVGGYRDDFDDPNSGWSMRRSTYLEEVKGFYENGRYVMQI
                     IDRWDWGIVSPLKPAPRLPYAIDFEMRSISQIYAHSAGGVFGGDWTGQTCPPGTSFDE
                     WYKHSACFNHFYNTNTIYNDSNASRVVLQLLFERIDRLEWCQGCGGSPMKRVGDTSDL
                     QNLRNVNPFEWNHYRIEVRQDGIKLYAGVPGQPLTLQYEYDDTRWVNSPYFGLFASTD
                     VIDNLTWRFEYVQVMPLD"
BASE COUNT         1999 a         3658 c         3490 g         1759 t
ORIGIN      
        1 tctccgtctg ccaccaggtg tccatgatgg ggcagcgctc cttgccgatg acgcgatagt
       61 accagcgcca cgcttccgga ttgatcggct cgccgacgga gccaagcagt cgcaggctgg
      121 acaggtcgtg tagctggggc cacgactcgc cgaagcgcat caggccgcgg atggctgtcg
      181 gcgcggtgta gaggatggtg atgcggtatt tgtcgatcat cgaccaccag cggtccgggt
      241 tcgggtgcgt tggcgcgccc tcgtacatga agctcgtcgc gcccaacacc agcggggcgt
      301 agacgatgta gctgtggccg gtaatccagc cggggtcggc cgcgcaccag tagatgtcgt
      361 gcggcttgat gtcaaaaacc catttcagcg tcgtcgccgt gccaaccatg tagccgccgt
      421 gggtatggag gatagccttt ggccggccgg tcgtgcccga cgtatagagc atgaacagcg
      481 gcgactcggc atccatgtgc tccgtcggcg ttcgcgggtc ggcaatgggc agagccatca
      541 ggtcgtgata ccagtggtcg cggtcggcca ccatctcgac cgcctgcccg gtgcgcttga
      601 cgacgatgac gctctgcacc gggttgttgc gctgcaacgc ctcgttaacg atgttcttca
      661 actcgacgat ctcgccgcgc agccaagccc cgtcgcaggt caccagcacc ttagaattgc
      721 tgtccagaat gcggtcatag agcgcctcga cagagaagcc gccatagacc accgagtgaa
      781 tcgcgccgat tttggcgcag gccaacatcg cgataaccag ttcggggatg cggcccatgt
      841 agatggtcac gcggtcgccc cggccgacgc cctgcgactt cagcacgccg gcgaacctgg
      901 acacttcgta gctcagttgc tgataggtga aggtgcgcgt gtcgcctggc tcgccctcaa
      961 agataagggc agccttgttg cgtgttgacg tgcgcaaatg gcgatcgagg gcgttgtgga
     1021 cgatgttgac ctgggcccca tcaaaccact tatagaatgg cgcgttgcta tcgtcgagca
     1081 ccatatccca gggcgcgtac cactcgaagc cctcggcggc gatacgcccc cagaaggcgg
     1141 acagatcggc cgcggcctcg gcatagacct tttcgtaatc gcgaatatgg gcatgggcga
     1201 cgacttccgg tgcggggtag tacacttccc cgaccatctg cttctccgta gacatgacgg
     1261 cctccaatgc gggatggaac ggtgaccaag gctgttaagc aacttttggc agcgtaagta
     1321 taatgggagc gggccaattc gtaaatgaat acaaacggac accttatgac accaccgaag
     1381 atcgtgataa tcggcgcggg cagcgccagc ttcggcccaa cgacgctggc gacgatcctg
     1441 cgcgacgagg cgctgcgtgg cagcgagttg gctctcgttg acctgaacgc ccgcagcgcc
     1501 gagaatgccg cccgcgtggc cgagcgcatg aacgccgcct ggggcgcagg catgaccatc
     1561 agcgccacga ccgagcggcg cgaggccctg cccggcgcga cgcacgtcgt cgtctctatc
     1621 gaggttgccc cgcgcgaggc gctgtggcaa ctggactggg aagtacccct gcgccacggc
     1681 ctgcgccagc cctacgctga gaacggcggg ccgggcgggc tgatgcacac ctgccggcaa
     1741 gttcccgccc acctggccat cgcccgcgac atggaagaac tgtgcccgtc ggcctggctc
     1801 atcctgttca gcaatccctt gccccgtctc gtgcgagcca tcaccaagta cagccgcatt
     1861 cgcgtcgtgg gcaagtgcca ccagatcaat gtcggctatg gcctcgtggc agccttgctg
     1921 cgcgaccgct acggcgtcga cgtgcccaac ggcgtaacgc tccactccga tccgggcaac
     1981 accggcgtcg tccacaccct ggccacggcc gggcggcgac acgtcagtat cacctcggct
     2041 ggccttaacc acttcatctg gctgctggat attcgcgacc gggccacggg cgaagacctt
     2101 taccccgccc tgcgcgccgc cgtcgctgac gccccgccga ccctggagcc gctgtcgctc
     2161 gacgtcttcc gcgcgttcgg tctgctgccc attgccgggg atacccacct ggccgagtac
     2221 ctgccctggc tccacgatcc tgtcgccaag ccgtgggaga cctaccggct gccgctctac
     2281 gactggcggg gcaacgaggg cgtgcgcgat gtcttgcagg gaatgatgca gatgctggcc
     2341 gatggcgtct tgccggttga agggatgcgc gaagcgccca gcgagggcgc ggccgaactc
     2401 atcgtcgggc tgaacggcca gacatacctc gacgagacgg tcaacgtgcc caattgcggc
     2461 gcgatcgccg ggctgccgcc cgagacaatt gttgaagtgc cggccatcgt tggcccgacg
     2521 ggcattcgcc cggctcaact gggccagttg cccccgccgg tggcggagct gctgcggcgc
     2581 gaggcggcac tggtggaagt ggtcgtcgat gccgccgcga ccggcgaccg cgacctggcc
     2641 cggcaagccc tgctgctcga cccgatgacc ggcgacatcg gccgcgcgcg agccatcctc
     2701 gacgactact tgcagacgtt cgcggcctat ctgccgcagt tcaagtaggg gcagacctgt
     2761 caggtctcac tggtcgtcga gacgtctccg tccacgggca caggggtggc gccgtcagcc
     2821 tcggccagcg gctcagcggc cgggtccggg aacaggtagc cgcagtaggg gcagatatcc
     2881 cagtgtaact ccagcggtga gcggcagacg gggcaaggct ggcgtagatg ggtgtggcag
     2941 gcagggcaga gaaaccagtt gggctgggtc ggccgggcgc aggtggggca aacaggcttg
     3001 tcctcgatct gctgcaatag cgcctcctgc tccagggcgt ggtcataggc tgcggccagc
     3061 gtctcgcgcg gccgcagcaa caagtagacc accaggccaa cgacgggcag cagggccacg
     3121 agcaggatgg tgagcacagc cgccacagtc gaacgcgagc gcgcgcggat atcgcggtag
     3181 ccccagacga ccagggccag ccacaccagg gccagcaacc caccggccgc gcctagcagt
     3241 atcgtcaggt agctctgtag actctccatc ggggccttac tcgcggccga ttgtaccacg
     3301 ccgccacgaa aaggcccccg gtttccgagg aaaccagggg ccgggttaac aaactgatca
     3361 gggggatcga cccggggcgc tagacgccct gctgctgctc tttctgcgac ttgcgcgtcc
     3421 gccgcgccgc tttcttcttg gcgatgcgag tcagttcgct cttggacaca aaccagcgct
     3481 tgcggcgcac gtcggtcagc acgcggctct tgacgacttc acggctgaag cggcggagca
     3541 gctgctcacc cgactcatcg ggtcgaagcg ttacctttgg cagagtattc accccctttg
     3601 ttttgaagtt tgctcatcag ggcggatgat agcacgggac cggcaacgcg gcaacctgac
     3661 gttggcagcg ccgtccaccg ggctataatc ccctggctcg ccatcaaccg gcgggcaatt
     3721 cacactcgga ggcaaccata tggaacccgt tggcatcgcc atcttcatcc tcttcatcct
     3781 gctcctggcg gcagcgatca ttctgcccaa gctgcaacgc aatcgcgcgc ggccgtaggg
     3841 tgtagatgaa gaaccgcagt aacttgtggc caaagggccg gctcgtgacc tgggtcgcgg
     3901 cggccctttt tctaaccatc gccgtccacc tcagcgccca gcctcctcct tcaaccgctc
     3961 tggccaacga acccaccctc gttgcccgcg tctactacaa cgacgccgcc gatctggccc
     4021 ggctgacaaa ctatgatgtc tgggaataca acaacgtcgc ccagcgctac gtcctggccg
     4081 ccgtcgctgg cgatgacctt gatgccctca gagcggccgg ctggcgcgtc gctgtggacg
     4141 agacggcgac cgcgcgactg gccgaccgcg ccgccaagcc gatgttcttc gacggttatc
     4201 gctcggtcga tgaactctac gcggccctcg acgcggcggc ggcggctcac ccggagttga
     4261 ctgagctgct cacctatggc cagagctatt gcctggccag cggcggctgc gtcacgcccg
     4321 gcggcgacgc cctggccggc tacccactgc gcgctatccg cgtcaccaat gaagccattg
     4381 ccggcagttc cctcatcagc ggcgcgaccg tcacgcgcgg cgagaaaccc gtctttttcc
     4441 tgatggccaa catccacgcc cgcgaaatca ccacgccgga gattgctctg cgcttcctcg
     4501 acttgctgct ggacggctac ggcacagacg ccaatgtcac ctggctgctc gactggcatg
     4561 aaatctggat cgtgcccacg gccaaccccg atggccactg gctggtggag ttgggcgagg
     4621 agcgtgccta tggcggcctg ccgttcatgc agcgcaagaa cgctcggttg ggcaacaaca
     4681 tcgacgggct tgacgactgt gacgcctggc caccggacgg cggtacgcag tttggcgtcg
     4741 acctgaaccg caaccacagc tttgcctggg gcggctccgg ctccagcgac gagccatgcg
     4801 accccgtgtt ccgcggccgc gcgcctgcct cagagagcga agtggcccgg ctggaagctc
     4861 ttctgaaggc cctctttgcc gaccggcgcg gcccagcgat ggacgaccca gccccggccg
     4921 atacggccgg tttgctgatc accctccaca gcttcagcaa cctcgtcctc tggccgtggg
     4981 gccatctgga cgtcgccgcg cccaacaaga ccgacctaca ggccatcggc gacaagctgg
     5041 ccacgttcaa cggctatcgc tcctgccagg cggcagccta tgactgcctc tacgccgccg
     5101 ccggggccac cgacgactgg gcctacggtg aactgggtat ccctgccttc actttcgaga
     5161 tgggcgacca attcatgcca tcttacaacg aggttgacgg cgtccagtgg cccgccaacg
     5221 gcccggcgct gctctatgca gccaccatcg cgcgcgcgcc ctaccaactc gcccatggcc
     5281 ccgacgcgcg cgatgtgctt gtggccgggc cggggccagt gcgctccgtg tcggccaccc
     5341 tcgacgcgac cagcaacagc ggcctgccca ttgccgcggc cgaatacacg gtggacgtac
     5401 caccgtggca ggaggaggcc ctccccgtgg ccctgacagc gacggatcat gtctttaacg
     5461 cggctgtgga gaaggtcgtg ggcgagatga acacctcggc gctgccgccc ggcggtcaca
     5521 tcctctacct gcgcggccag gacagcgccg gcaattgggg gcctgtgtca gccgcgttca
     5581 ttacggttga cgccacgccg gacacgtggc tctatctgcc cagcgtgcta gggccataga
     5641 catggggccg ccggcgcggc gggttgcccg tgttgtgtgc agcccgccgc gtttgcctgc
     5701 ggctaaacct gcgcccggaa ttgggtctcg tagagagacg catacaggcc gccgccggcc
     5761 agcaattcgt cgtgcgtgcc ccgctccacc agccgcccct cgtccatgac cagaatgaca
     5821 tccgcggcca gaatggtcga taagcggtgg gcaatgacca ggctggtgcg cccctgcatg
     5881 acgtgctgca gggcatcctg gatgagtgcc tctgatagcg agtcgaggtg agatgtagcc
     5941 tcgtcgagga cgaggatgcg cgggttcttg aggatgacgc gggcgatggc gacgcgctgg
     6001 cgctcgccac cgctcagccg gtagccgcgc tcgcccacaa ccgtctcata gccgtcgggc
     6061 agcccggcga tgaagtcgtg gatgttggcc gcgcgggcgg cggcgatcat ctcggcctcg
     6121 gtcgcgtcgg gacgggcgta gagcagattg gcgcggatgg tgtcgtagaa caggtaggtc
     6181 tcctgggtca ccataccgat gttctcggcc agcgagttta gcgtcacgtc gcgcaggtcg
     6241 tggccgtcga tgagcacccg gccaccggtg gggtcataga gccgggggat gagataggtg
     6301 accgtcgtct tgcccgcgcc gctcggcccg accagcgcgg ccaactgccc cggctcaatg
     6361 cgaaagtcca cgtcgcgcag cgccacccgc cgcagcgtgc ccggctcgga gccgttcgtc
     6421 ccattgccgt tggccgcggc gacttcggcc ccgtctggct ccccgtcggg gcgataggca
     6481 acaggccggg gtttgcggcc ttgcccgcgg cgcaaaagcg tttcgccgct gccccaatcg
     6541 tagcgcacta cctcggccag ggcggccttc ttttcgcccg acgcggcgta gtcaaaggtc
     6601 acctggtcga aagcgatctg cccggcgaca tcgcgcagcg gcacagcgtc cggctcttcg
     6661 gcgatctcca gcggaatatc cagtacctcg aagacgcgct cgaagctgac catgctctgg
     6721 gcaaactcca ccggcgcatt ggtcagggcc atgagggggc cgtagagctg gctgaggtag
     6781 gcgccaaaag cgacgatggt accaatagtg aacgcgccct gaatggtcag gtagccgcca
     6841 acccagtaga ccagcgcgct gccgaccgcg ccaatgatgc ccagaaccat gaagaaccag
     6901 cggccgatca ccgccgagtg gacgccgagg tcgcgcacag cggcgctgtc gcgccgaaag
     6961 cgggccagct cgcgctcctc gcggccgaac agctttacca gcagcgcccc gctgacgttg
     7021 agcgtctcgt tcatcgtcgc gttcatctcg gcgttgagaa ccatcgaacg acggcgcagg
     7081 tcgcgcagga cgcggccgat acgccgcgcc ggcaagtaga agaacggcaa cacgaccaac
     7141 cccagcagcg ttagccgcca ctccagagcg agcatgatcg ccagcgtgcc gatcacctgg
     7201 acgatgttgg agaagatggc gatcagcgtg ttgctgacgg cctgttgcgc cccgacgacg
     7261 tcgttgttga gccgcgacat cagctcaccg gtgcgcgact gcgtaaaaaa gcgcaaggac
     7321 atgcgctgga agtgctcata gacggcacag cgcaggtcga aaatgatgct ttcgccgacg
     7381 aaggcactca gcttgcgctg ccagacgccg acgacgccgt tgagaatggg cacggtgatg
     7441 agcgccgcgg ccagccaggc caaccggcgc gtgtcggcat tgggaatcac gtcgtcgatc
     7501 agcgtgcgca tcaacagcgg cgtgaccagg gtaatgcccg tcgttagcaa gatgctaagc
     7561 aacagcagca cgacctcgcg attataagga cgtgcaaact gccacacacg ccccagcagg
     7621 tcacgagtca ccaccggccg atcttgctct tcatcctggc tgaggaaggc ccaccagcca
     7681 ccgccatgaa agcccataag ttactccata actcccctag accacgctac ggaatcgcga
     7741 actgacccaa ttctacgctg tctcggccat cgggaaacct ccacctttgg ttagtcaaag
     7801 ggtcatagag gccagcgacg acgcgatacg tgccggacgg agccgattcg ggcaaggtga
     7861 ccgtcgccag ttgcaccagg gtgtcccccg gccgccagcc ctcccaggcg gcgctcagcc
     7921 cgtcccactg agccatgagt tgcccgctct ggtcgagcac gtggatgaaa atctggatcg
     7981 gccggggcgc gccctcctgc cgccagatgg tcagcgtagt aatagcgcgg ccgtcctgtc
     8041 gcgcccaact gtcctggcca taggcgatag cgttgccgtt gagcgagaga ccggcctcag
     8101 tcgcgggcgg gcgggccacg gcgcggaaga ggcgatagcc gtcgccagcc gccacggccg
     8161 ccggcccata gtccaggaag gggcgcagcg ccgccggcac gtcctcctcg gccggcaaca
     8221 gcagccacag cggggcgacg ccggccggca acaccagcgc ccggtcggcc tcgaaccacc
     8281 gcggcgtcac ctggttggtg cccagttggg cgaagtcggc cgcggccagg ctatccatct
     8341 gcgtgctggc cagcagcaga tcgaccggct cgccgcgtgg cgcgacccgg tagaggaaga
     8401 tgctgtagcc caccttgtcg tcggccggcc gctcgcggaa gaaacggaac tggtcgtggt
     8461 cggcgaagtg gacaccttgc agggtcgtcg cgctgatggc gtaccaaccc ggcgcgggat
     8521 cgtggggata gaacggccgc gcgacgggat tcatcagccg gggcggaaag ctatccagcc
     8581 cgtcataggc gatgccatag tactctggcc ggccctcacc aaagtagctc agccagacac
     8641 gcgccacgcc acggtcagcc atccacgtct tcagccggcc caggtcttgc ccccagtcga
     8701 tattgctatc caccagcgcc cgccagccat tgtccggccc gccggccagg acgttgaaga
     8761 aggagatgaa gtgcggatag acccacaccg tcgccaatgc cagccaggcc acgccggcgg
     8821 tgacgaccac ggccgtccgg ttgcgccccg ccaaccctgg tgcctgactg gcccgcttgc
     8881 gggcggtttg ggccacgacc gcgccaatca gcaccatcag aaatggcagc accgccagca
     8941 gatggcgata gccgagattg atgtccgttg tcagggcgat ggcgaagtaa cccccggcgg
     9001 gaatgagcag cgccgccagg tcgaagcctg ctatctgaaa gcgatcccgc cggcgcggga
     9061 tcattgacgc gaggcgaaat atggccagac caaacagcag cagcgttggc agcggcgttt
     9121 tcagcgcgaa ggcgaccggg aagtaatacc accagccggt gtctgagtag cgccccagca
     9181 ggaacgacgg cgcgcccact tgcatccggt tgccaatggc cagaagttgc gcaaagtagt
     9241 cggccaatgg cacgggcgcg ccgcccaaca agcccaccac cggcagcggc gcgcgcaatg
     9301 gcccgatctg aaagccactc gtcgcccaga gcgtcagcaa agccacggcg ggatagacga
     9361 gaagcgtttg cagaaggagg cgacgatgga gcgtccgccg ccgcgtccac agcgccagga
     9421 aaatgaccac gccaaagaga ggcacaaaca gcagcgcggt gaacttggtg ttctgcaaca
     9481 ggcccagccc catcccggcc aggatcgccg cgggccacga cggccgccgt agaaaacgcc
     9541 agagcgtata gccggccagc gcggcccccg cggccaaccc caggtcagtc gtcgccaggc
     9601 gcgtgtgggc caggatgttg ggatcgagcg cggccagggc cagcgccagc agccccgccc
     9661 cgttggcggc cgaccgcccc agccgccacc cgccggccat ctgccccgcc cagcggccga
     9721 ccaccgcggc catcagcatg cccagccaga tcgttggcac gcgggccagg aacatcgtcc
     9781 gggcgacatc gttgccgcgg ccccacaaaa acagctcggc cggccggtgg aagctcgtct
     9841 cgccccagga gggatcgtcc gagggcagcc ggacggtcgg atcggccacc agcaagacag
     9901 cgttcagcgc gttgaggccg atggggtggc cgacgatgat cgtccgcgtg ccgccgtgtt
     9961 ccggcccgcg cagatagccc agcccgcgca cgaggaagcc ctgttcgtca aacgttggtc
    10021 cctcgccgga catgctggtc agcgccaggg caaacatgac ccatagcagc agaacggcca
    10081 cacaggccgg ccgggacaac agttggcgca cgacgctttc tccccataga aatggccggc
    10141 aagtccccgt aagggttctt gccggccact ggttcacgaa ccgtctaagc caattagtcg
    10201 agcggcatta cctggacgta ctcgaaccgc caggtgagat tgtctatgac gtcagtcgac
    10261 gcgaacaaac caaagtacgg gctattgacc cagcgggtgt cgtcgtactc gtattgcagc
    10321 gtcagtggct ggccgggcac gcccgcgtaa agcttaatgc cgtcctgccg cacctcgatg
    10381 cggtaatggt tccattcaaa ggggttcaca ttgcggagat tttgcaggtc gctcgtgtcg
    10441 cccacacgct tcatcgggct gccgccacag ccttgacacc attccagccg gtcgatgcgc
    10501 tcgaacagca gttgcaggac gacgcgactg gcattggagt cgttgtaaat ggtgttggta
    10561 ttgtagaagt ggttgaaaca agcgctgtgt ttgtaccact catcgaacga ggtgcccggt
    10621 gggcaggtct ggccagtcca gtcgccgccg aagacgccgc cggccgagtg ggcgtagatc
    10681 tggctgatac tgcgcatctc gaagtcgatc gcatagggta ggcgcggcgc tggtttcagc
    10741 gggctgacaa tgccccagtc ccagcggtcg atgatctgca tgacgtagcg gccgttctca
    10801 tagaagccct tgacctcctc aagataggtc gaacgacgca tgctccagcc gctgttggga
    10861 tcgtcgaagt cgtcgcgata gccgccaacg atctggccca cattcg
//
LOCUS       DAOSMQ010000037        10905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6006_length_10905_cov_4.239539, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000037 DAOSMQ010000000
VERSION     DAOSMQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6006_length_10905_cov_4.239539"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1513
                     /locus_tag="PK829_02225"
     CDS             95..1513
                     /locus_tag="PK829_02225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU40062.1"
                     /translation="MIVVVIALYMVSVAGLALYGLLGFVTLALFLRYRHGQQPRVPVA
                     PAEWPVVTVQLPLYNERGVVTRLLEATAALDYPRDCLQIQILDDSTDDTTALAAAWVA
                     TRQAEGYDAILLHRAERQGYKAGALGNGLDTARGEFIAIFDADFAPQPSFLRQTLPYL
                     LDDPHLGAVQTRWGHLNATESALTGAQAIALDKHFAVEQLVRHRAEYFPKFNGSAGVW
                     RKTCIQAVGGWSAETLCEDLCLSTRAVLAGWRFHFADDIVAPAELPRTIRAYKSQQAR
                     WATGASQCLARYAAPIWRASDHSPLARLYALLSMSAYCTHLLLLLLLLVQLPLILSGV
                     RFPAWMVLFSALGLGQPILFILAQQFLYPDWLRRLRYLPALLLLAIGTAPGNAWAVLR
                     GLSGRRFVFVRTPKGAQPGYRLKAGNGVWAEAGLAVYSAVLLVLATWTGNTGAASLPL
                     ISLLGFGYVAWLTGRESWLAAS"
     gene            1673..2062
                     /locus_tag="PK829_02230"
     CDS             1673..2062
                     /locus_tag="PK829_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOU40063.1"
                     /translation="MNPVIGQCPICGNHLHVTRLQCRNCDTAIEGHFTLGRLYELSPE
                     QLEFVEIFLRCEGKINRVEQEIGLSYPAVRARLNDVIRALGYEVGEPPAASRGVPEEV
                     RREVLADLRAGKLSADEALQILRGDGD"
     gene            2184..2651
                     /locus_tag="PK829_02235"
     CDS             2184..2651
                     /locus_tag="PK829_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40064.1"
                     /translation="MDRLAAGQISVSEAMRQLDRTAAPASVESLKAEEQAADAIPVEE
                     LKAEEAVAIRMPEEPAILKEAIANGNTAPADGKPRWLKIRVRDTASDNNKVTVSLPLG
                     LVSFGLGIAQRFSADMAGVNTDEIMALLKSGQRGVLVEVQDDEDNEHVQIYVD"
     gene            2662..3885
                     /locus_tag="PK829_02240"
     CDS             2662..3885
                     /locus_tag="PK829_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40065.1"
                     /translation="MAKERFETGKAPEIHVAACDGDLVVRGWAESMLQVRGGYEVEES
                     PEGYRLSGRGDLRLLVPAGANVAIDEVSGDLVVKGLTGQGAARQVHGNAILVEVGAFN
                     SEMVHGNLVARGVQSLAAGAVHGDVSARRVGGVKLGAVYGDFSGKGLDGSVTIEEASG
                     DVSVREVNGEIALGHAHRDVNLAAVAGRVMLGSVDGDIRLRGALPPGDHALSAHGDIV
                     VRWPANVAVNVVAAAKRIVNRLPLQDVAEKDGQLLGRIGNGTTQLTLSADGQIVLKEI
                     TPVDEKWDDGMIGDDAEFEPFFSGLGLENMTARIEAEVNNHLARVARDIETRFGPEFG
                     QRMADKVARQAERATERARRKGEWRSRAGDSATAAAPQRRSASPEEQLKILKMVESGA
                     ITPEDAGMLLEALEG"
     gene            4024..5007
                     /locus_tag="PK829_02245"
     CDS             4024..5007
                     /locus_tag="PK829_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40066.1"
                     /translation="MVITVPDTRANEGLRPIDMARDVPQIVELLKLVFGEALELDEGQ
                     LLGDDVGAQISNLFYRLNPATSRLANGFVWVAEGRIIGNVTLLSTKAWDRYLVANVAV
                     HPTYRRRGIARAMMQAVTTHVRARGGRAILLQVVKDNTSAIDLYRSLSYVRVGDMTTW
                     YSSASRLRRLEPANDAPPIDIRPLPGNRWRDAYALDTTCVPADLNWPEPLRTDAYRQT
                     LWQRLGDMMNSRMLETWVTTGASDRLTGLMSISGQWGRSHWVVLRIHPDYAGQLERPL
                     LAKAIRRLQQLPRRNVRIDHPDDDTTTTALLAEANFAVQRTLTHMRLDLSR"
     gene            5028..5411
                     /locus_tag="PK829_02250"
     CDS             5028..5411
                     /locus_tag="PK829_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40067.1"
                     /translation="MATTEERLQILKMIEEGKISAAEGAELLRALDTKGDRSQPMEPM
                     KGTSSPRWFRVRVTDVQSGRNKVNVNIPMGLVNVGIKMGARFAPQIEGKQLETVLEAV
                     RSGQLGKIVDIVDTEDGERVEIFVE"
     gene            5861..6811
                     /locus_tag="PK829_02255"
     CDS             5861..6811
                     /locus_tag="PK829_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOU40068.1"
                     /translation="MNQYDAYEKYQEFDDYQTEKSAYRKAKAGKKKPKPAEMIAQLSN
                     FDDSLAAWVPSYAAALDPRHHERQWLIESVGPFYRDNVITDVTHRVKSGKEANVYCCT
                     AHPASGVALIAAKLYRPRMLRTLKNDIIYKAGRQLRGEDGKLMKGRREKVALAQKTNF
                     GKQLDMTWWIQNEFSTQQKLFEAGAAVPRPIGHNGNAILMDYIGEEGLPAPALIDSAP
                     APGEARDLFALIMDNVQLMLNNHLVHGDLSAYNILYWEGTAVIIDFPQMVDARANPHA
                     YELLRRDVARVCAYFARFGVRADAEELARDLWLPYMGSIG"
     gene            6898..7251
                     /locus_tag="PK829_02260"
     CDS             6898..7251
                     /locus_tag="PK829_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40069.1"
                     /translation="MAVSTKGKRKVNVRGRQYIWYVSESGRTTAPEQGFVVEGDPARI
                     LHIIASNKQFIVHYRLPQTGDTAAQLTVEGPDIPRAPGARQVEVPRWRHDSNPYPTAD
                     FVRRLIGWCLGLEQT"
     gene            7302..7910
                     /locus_tag="PK829_02265"
     CDS             7302..7910
                     /locus_tag="PK829_02265"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002744503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU40070.1"
                     /translation="MNTAEQTLRSAGTQTKRLLRPLLIILALMWIIEIADWLLFNGAL
                     DQFGIIPRQLVGLRGIPIAPLLHGSFSHLAANTIPFLALGLLVMLRHSRQFTAVSIII
                     VLVSGLGTWLFAPAYTIHIGASGLIFGYFAFLVVAAFYERSPGAIGLALLVIVFYGGL
                     LWGAMPQDGAISWQGHLFGLVGGGLAAYAVAPRRIRIRIDPN"
     gene            complement(7907..8710)
                     /gene="tatC"
                     /locus_tag="PK829_02270"
     CDS             complement(7907..8710)
                     /gene="tatC"
                     /locus_tag="PK829_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOU40071.1"
                     /translation="MTTDERRDRNRPDAEGPVMSILDHLNELRVHVMRAGIAVLVTTI
                     FSFIFAERLLAFLLQPYAQSRPAGATLQTLRPTEGIETYFRVALLAGIILAMPIILLE
                     LWRFIQPGLSRQERRYVYIFIPAGLALFLLGIAFAWYVLAPTAIYFLANFMTEVFATE
                     WTGDEYIGFITRLLLWIGLGFQMPIVIYFIARLGLLTARTMREQWRVAVVVIAILAAV
                     ITPSIDPVTMLLTMAPLLALYVLSIGLAVLGQRQFERSAEASEVPISTA"
     gene            complement(8713..9258)
                     /locus_tag="PK829_02275"
     CDS             complement(8713..9258)
                     /locus_tag="PK829_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40072.1"
                     /translation="MDSVFGIGLPELILVVVLAGMVMGPERIVRYARMLGVVTARLQS
                     VSRAFVHQLTAELDAADSSGELRETIDELQLLRRQVAELRAEVLTIASGTAAEGKQVM
                     RELKQAQNAIMPPSLLAATREARATTAPTPISAATPGPAVHRPPSLFPEATPSAPPAT
                     GTPPALSPALPRRVDITDDPD"
     gene            complement(9268..9468)
                     /gene="tatA"
                     /locus_tag="PK829_02280"
     CDS             complement(9268..9468)
                     /gene="tatA"
                     /locus_tag="PK829_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOU40073.1"
                     /translation="MLPGGWEWIIILVIVLLIFGVGRIARVGGELGKGVSAFRQGLKE
                     GQEEKEAAEKKPEGTGDTLPKS"
     gene            complement(9567..>10905)
                     /locus_tag="PK829_02285"
     CDS             complement(9567..>10905)
                     /locus_tag="PK829_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU40074.1"
                     /translation="GEPVTAEDVAFTYGLMQSDDFPGPAELIPLWQSATITVIDDHHI
                     QFTLAQPYAPFIEATTRGILPAHLLGDIKPADLDGIAFNAAPIGTGPFMVQPGQDWQQ
                     SGRLRLTPNPIDWREGTLLSDIEFRFFATADALLTAFHNGEIHAANNLPPTLAPDVLT
                     AEGVRLFSSPAQRYTALFFNLGDTGTPAARSKEVRQALAYGLDRAALIATVLNGQGLV
                     FEGPYLPDSWAARPGLMTAYSYQPETAAALLDTAGWPFGDGGRFHDGGLLTLRLVAVD
                     QPEPRAVAQEIARQWAALGIDTPVTLLPDADSLRAALDSGDFDVALLDVVPPADPDLY
                     DFWSQEAIVRGHNYGRWNNRRASEALEAARQLMPQAERAVYYEAFLRQFDADLPALTL
                     YQHVTGYVLSDAVQAAEIGRLQGPRDRFATLPAWFLSYREVPVNCPAATPAGG"
BASE COUNT         1997 a         3546 c         3472 g         1890 t
ORIGIN      
        1 ctgctgccgt tgtcgccggc gcgtgctgtt gccgcgcggc cagttcaaca agcaactcaa
       61 ggccatcatc gcccacgctc ccaaagccga ctgaatgatc gttgtcgtga ttgcccttta
      121 tatggtcagc gtggctggcc tggcgctcta tgggttgctg ggctttgtca ccctggccct
      181 attcctacgc tatcggcacg gccagcagcc tcgcgttcct gtcgccccgg ccgagtggcc
      241 ggtcgtcacg gtccagctgc cactctacaa cgagcgtggc gtcgtcaccc ggctgctgga
      301 ggctacggcc gccctcgact acccgcgcga ctgcctgcag atccagatac tggatgactc
      361 caccgacgac accacggcgc tggccgcagc ctgggttgcc acgcggcagg ccgagggcta
      421 cgacgcgatc ttgctgcacc gcgccgagcg acaggggtac aaggccggcg ccctgggcaa
      481 tgggctagat acagcccgcg gcgaattcat tgccatcttc gacgccgact tcgcgccaca
      541 gccgtccttc ctgcgccaga cactgcccta tcttcttgac gacccacacc tgggcgcggt
      601 gcagacgcgc tggggccatc tgaacgcgac cgagtcagca ctgaccggcg cgcaggcgat
      661 tgccctcgac aagcacttcg ccgtggaaca actcgtgcgt catcgcgccg aatacttccc
      721 caagttcaat ggctccgccg gcgtctggcg caaaacctgc atccaggccg tcggtggttg
      781 gagtgctgag acactatgcg aagacctatg cctcagcacg cgggccgttc tggccggctg
      841 gcgcttccac ttcgccgatg acatcgtcgc cccggccgag ttgccccgca cgattcgcgc
      901 ctacaagagc cagcaggccc gctgggccac gggcgccagc cagtgcctgg cccgctacgc
      961 cgcgcctatc tggcgcgcgt ccgaccactc gccgttagcc cgcctctatg ccctgctgtc
     1021 gatgtcggcc tactgcaccc acttgctgct cctcctcttg ctgcttgtcc agctaccgct
     1081 catcctgagc ggtgtccgct ttcccgcctg gatggtcctc ttcagtgccc tgggcttggg
     1141 ccagcccatc ctcttcatcc tcgcccagca gttcctctac cccgattggc tccggcggct
     1201 gcgctatctg ccggctctcc ttctgctggc catcggcaca gcgccgggca acgcctgggc
     1261 agtgctgcgc ggcctctctg gccggcgctt tgtctttgtg cgcacgccca agggcgcgca
     1321 acctggctac cggctcaagg ccggcaacgg tgtatgggca gaggcgggcc tggccgtcta
     1381 ctcggccgtc ttgcttgtgc tcgccacgtg gacgggtaac acgggcgcgg cgtccttgcc
     1441 gctgatctcc ctcctcggtt ttggctacgt tgcctggctc accggacgcg agtcatggct
     1501 tgccgccagc taatgcccct ttagccccct ggtcacactt tcatatttct gcacatcgat
     1561 attagatttt tcaatctagg tattgacatt tcttatatcc gattgtatta tctgaacaga
     1621 tcgttgaaga actgatcgat ctgtaaagat tctttagccg gaacgagaga aaatgaaccc
     1681 cgtcattggc caatgcccca tttgcggcaa ccacctacat gttacccggc tccagtgtcg
     1741 caattgcgac acagccattg aaggccactt caccctcggc cgcctttatg agttaagccc
     1801 cgaacagctg gagttcgtcg agatcttcct acgctgcgag ggcaagatca accgcgtgga
     1861 acaggagatc ggtctgtcat accccgctgt tcgcgcgcgc ctgaacgatg tcattcgcgc
     1921 cctgggctat gaggtcggcg agccgccggc cgccagccgg ggcgtgcccg aggaggtgcg
     1981 ccgcgaagtg ctggccgacc tgcgagcggg caaactgtcg gccgatgagg cgctgcagat
     2041 cctccgcggc gacggcgact gagcacaacc agcatcgccg gcggccgcgc gccccggcgg
     2101 gcaaccgatg cagcggccac tgcgaatggc cactgcgaca gtgggagatg aatgaaatga
     2161 atgcagcgga acgtgcggaa atcttggaca ggctggccgc aggccagatc agtgtcagcg
     2221 aggccatgcg ccaactcgac cggacggccg ccccggcctc cgtcgagtcg ctcaaggccg
     2281 aagagcaggc cgccgacgcc atcccggtgg aagagttaaa ggccgaagag gccgtcgcca
     2341 tccggatgcc cgaagagccg gccatcttga aagaggccat cgccaacggc aacacagcgc
     2401 cggccgatgg caagccgcgg tggctgaaaa ttcgtgtccg cgacacggcc agtgacaaca
     2461 acaaggtcac cgtctctctg ccgctgggtc tggtcagttt cgggctaggc attgcacagc
     2521 gcttcagcgc cgacatggcc ggcgtgaaca ccgacgaaat catggccctg ctcaagtccg
     2581 ggcagcgcgg cgtccttgtg gaagtgcagg acgacgagga taacgaacac gtccagatct
     2641 acgtggacta ggaaggatca catggccaag gaacgtttcg agacgggcaa ggcgcccgag
     2701 attcatgtcg ccgcctgcga cggcgacctg gttgtccgcg gttgggccga gtcgatgctg
     2761 caagtgcgag gcggctacga agtggaagaa tcgccggagg gttaccggtt gagcggccgg
     2821 ggtgacctgc gcctcctggt gccggccggg gccaacgttg ccatcgacga ggtcagtggc
     2881 gaccttgtag tgaaggggct taccggccag ggcgcggccc gccaggtgca cggcaacgcc
     2941 atcttggtcg aagtcggcgc tttcaacagc gagatggtcc acggcaacct ggtggcgcgc
     3001 ggcgtccaga gcctggccgc cggcgcggtg catggcgacg tatcagcgcg ccgcgtcggc
     3061 ggtgtcaagt tgggcgccgt ctacggtgac ttcagcggca aaggtctaga cggctccgtg
     3121 accattgagg aggcttccgg cgatgttagc gtccgcgagg tgaacggcga gattgccctc
     3181 ggccacgccc accgggatgt gaacctggcg gccgtcgccg ggcgcgtcat gctgggcagc
     3241 gtggacggcg acattcgcct gcggggcgcg ttgccgcccg gcgaccatgc cctctccgcc
     3301 cacggcgaca tcgtcgtgcg ctggccggcc aatgtagccg tgaatgtggt cgccgccgcc
     3361 aagcgcatcg tcaatcgcct gccattgcaa gacgtggccg agaaggatgg tcagttgctg
     3421 gggcgcattg gcaatggcac aacgcaactg acgctttcag ccgatggcca gattgtcctg
     3481 aaggagatta cgccagtgga tgagaagtgg gacgatggca tgatcggcga cgacgctgag
     3541 ttcgagccgt ttttcagcgg cctggggctg gaaaacatga ccgcgcgcat cgaggccgag
     3601 gtcaataatc atctggcgcg ggtggcgcgc gacatcgaga cacgcttcgg cccagagttc
     3661 ggccagcgca tggccgacaa ggttgcgcgg caggcggaac gagcaaccga gcgcgcccgc
     3721 cgcaagggag aatggcgcag ccgcgccggc gacagcgcga cagccgccgc gccacaacgc
     3781 cgatcggctt cgcccgaaga gcaactcaaa atcctcaaga tggtcgagtc gggagcgatc
     3841 acgccggagg atgcaggcat gttgctggag gcgctcgagg gataagcgat gtcgttggct
     3901 ctcgtctcgc acccggacta caatagcaac cgtcggccgt cgggcggcca tcccctgccc
     3961 tgtgcgacct gggcacggcc ctttgccggc cgtcctgacg gtgcgagacg agagcgcaac
     4021 gcaatggtca ttaccgttcc agatacacga gcgaatgaag gtctgcgccc tatcgacatg
     4081 gcgcgcgacg tgccgcagat cgtcgagctg ctcaagttgg tctttggcga agcgctggaa
     4141 ctcgacgagg gccagctgtt gggcgacgat gtcggcgcgc agatcagcaa tctgttctac
     4201 cgcctcaatc cggccacatc gcgcctcgcc aacggctttg tctgggtggc cgaagggcgc
     4261 atcatcggca atgtgacctt gctatcgaca aaggcgtggg accgctacct ggtggccaac
     4321 gtcgccgtgc acccgaccta tcgccggcgc ggcattgccc gggcgatgat gcaagccgtc
     4381 accacccacg tgcgtgcccg cggcggtcgg gccatcctgc tccaggttgt taaggacaac
     4441 acctcggcca tcgatctcta ccgctcgctg agctacgtcc gcgttggcga tatgaccacc
     4501 tggtacagct cggcctcgcg gctgcgccgg ctggagccgg ccaacgacgc tccccccatc
     4561 gacatccggc cgctgcccgg caaccgctgg cgcgacgcct atgccctgga caccacgtgt
     4621 gtgcccgccg atctcaactg gccggagccg ctgcgcaccg atgcctatcg ccagacgctg
     4681 tggcagcgcc tgggagacat gatgaacagc cgcatgttgg agacctgggt cacaaccggc
     4741 gcaagtgatc gtctgaccgg actcatgtcg atctccgggc aatggggccg cagtcattgg
     4801 gttgtgctgc gcatccatcc cgactatgcc ggacagttgg agcgcccact gctggccaag
     4861 gccatccgcc gtctgcaaca gctgccacgg cgtaacgtgc gcatcgacca tcccgacgac
     4921 gataccacta ccactgccct gctggccgaa gccaattttg ccgtccagcg gacactcacc
     4981 cacatgcgac tggatctatc gcgctagtgg gcaaggggaa acaaattatg gctaccactg
     5041 aggaacgcct ccaaatcctg aagatgattg aagagggcaa gatcagcgcc gccgaaggcg
     5101 ctgaattgct gcgcgccctg gacacgaagg gcgaccgcag ccagccgatg gaaccgatga
     5161 agggcaccag cagcccgcgt tggttccgcg tgcgggtgac agatgtgcag tccggccgca
     5221 acaaggtcaa cgtcaacatc ccgatggggc tggtgaatgt gggcattaag atgggcgctc
     5281 gttttgcgcc acagatcgag ggcaagcagt tggagactgt gctggaggcc gtccgcagcg
     5341 gtcagttggg caagatcgtc gacatcgttg acaccgaaga cggcgaacgg gtcgagattt
     5401 tcgtcgaata gcacaccagc gcgcgccgac ctgacaggtg ggcgaccaaa acctttcccg
     5461 cggaagtggg ccatcgggcc gcctacctgt caggtcattg ggcaaaatcc gaaaatccga
     5521 gcgcctcttg acgggcgcac ggcgactatg ctagactgtc tttggctcag aggtctgacc
     5581 caaatctttt agcggaggtg cacaagcatg caagccaacc agaatgtctc tgaacatacg
     5641 gttgtccgtc gctgcgtcgg cgcgtccgct cctctccgct agttcctccg atttcgatcg
     5701 tcaatcgaac ccagcggtcg cggacagctg aaagccggat gcatgggcat ccgtcgccgg
     5761 cactgccgtc tcttatcgct ggctgcggcc cacgcgggcc acggccgacc tggcgcttcg
     5821 ccagccggcc gcatccgttt cgattgtttc gaaattggca atgaatcaat acgacgctta
     5881 cgaaaagtat caggaatttg acgactacca gactgaaaag agcgcctatc gcaaggccaa
     5941 agcgggcaag aagaagccca agccggccga gatgatcgcc caactgagca actttgacga
     6001 cagcctggcc gcctgggtgc ccagctatgc cgctgccctc gacccgcgcc accacgaacg
     6061 ccagtggcta atcgaatctg tcggcccctt ctaccgcgat aacgtgatta ccgacgtgac
     6121 ccaccgggtc aagagtggca aggaagccaa cgtctactgc tgcaccgccc atccggccag
     6181 cggcgtcgct ctgatcgcgg ccaagcttta ccgcccgcgt atgttgcgca cgttgaaaaa
     6241 cgacatcatc tacaaagccg gccggcagct gcggggcgaa gacggcaagc tgatgaaggg
     6301 ccgccgcgaa aaggtcgccc tggcccagaa gaccaacttc ggcaagcagc tggacatgac
     6361 ctggtggatc cagaacgagt tcagcaccca gcaaaagctg ttcgaggccg gcgcggccgt
     6421 tccccgcccc atcggccaca acggcaatgc catcctcatg gactacatcg gcgaagaggg
     6481 cctgcccgcg ccggcgctca tcgacagtgc ccccgcgccg ggcgaagcgc gtgatctctt
     6541 cgcgctgatc atggacaatg tgcagctgat gttgaataac catctggtgc acggcgacct
     6601 gtcggcctac aacatcctct attgggaggg cacggccgtc atcatcgact ttccacagat
     6661 ggtcgacgcc cgcgccaatc cccacgccta cgaattgttg cggcgcgacg tggctcgtgt
     6721 gtgcgcctac tttgcccgtt tcggcgtgcg cgccgacgcg gaagagttgg cgcgcgatct
     6781 gtggctgccg tacatgggca gcatcggcta gagcgggccg ccacggccgc ggccgtggac
     6841 gggatgccgg ccggatgccg gccggcatcc catccagata gctcaaggag tgtacgattg
     6901 gcagtcagca ccaaaggcaa gcgcaaggtc aatgtacgcg gccggcaata catctggtat
     6961 gtcagcgaga gcgggcggac gacagcgccg gagcagggct ttgtcgttga gggggacccg
     7021 gcccgcatcc tgcacatcat cgccagcaac aagcagttca tcgttcacta ccgcctgcca
     7081 caaacaggcg acacagcggc gcaactgacc gtagaagggc cggacatccc ccgcgcgccg
     7141 ggcgcacgcc aggtagaagt gccgcgttgg cggcacgaca gcaaccccta ccccacggcc
     7201 gacttcgtgc gccggctcat tggctggtgc ctggggctgg aacagacctg agagcggtcg
     7261 ccccggttgc gaccgtggcg cgacagttgc gtaatgtcca tatgaacacc gccgaacaaa
     7321 ccctccgatc cgccggcacg cagaccaaac ggctcctgcg cccccttttg atcattctgg
     7381 ccctgatgtg gatcatcgag attgccgact ggctgctgtt caacggcgcg ctcgatcagt
     7441 ttggcattat tccccgccag cttgtcggtc tgcgcggcat ccctatcgcg ccactgctcc
     7501 acggcagctt tagccatctg gccgccaaca ccattccctt cctggccctc ggcctgctgg
     7561 tcatgctgcg ccacagccgc cagtttacgg ccgtgtcgat catcattgtg ctggtcagcg
     7621 gcctgggcac atggctgttt gccccggcgt acacgatcca cattggcgcc agtgggctga
     7681 tcttcggcta cttcgccttt ctggtcgtgg cggccttcta tgagcgcagt ccaggcgcga
     7741 ttggcctggc gctactcgtc atcgttttct atgggggact gctatggggg gccatgccgc
     7801 aagatggcgc tatctcctgg cagggccatc tcttcgggct ggtggggggc gggctggcgg
     7861 cctatgccgt cgccccgcgg cgcatccgca ttcgcatcga cccgaattag gccgtcgaaa
     7921 tgggcacttc gctggcttcg gcgctgcgct cgaactgccg ctggcccagc acagccaggc
     7981 cgatgctgag cacatacagc gccagcagag gggccatcgt cagcaacatc gtcaccggat
     8041 cgatggaggg tgtgatgacg gccgccagga tggcaatgac gacgacagcg acgcgccact
     8101 gctcgcgcat ggtgcgcgcc gtcagcaggc ccagccgggc aatgaagtag atgacgatgg
     8161 gcatctggaa gccgaggccg atccacagca gcagccgggt gataaagccg atatactcgt
     8221 cgccagtcca ctccgtggcg aagacttcgg tcatgaagtt ggccaggaaa tagatggccg
     8281 taggggccag cacataccag gcgaaggcga tgcccagcag gaagagcgcc agcccggcgg
     8341 ggatgaagat gtagacgtag cggcgctcct gccggctcag ccccggctgg atgaagcgcc
     8401 acaactccag caagataatg ggcatggcca gaatgatgcc ggccagtaac gccacgcgga
     8461 aataggtctc gatgccctcg gtcggacgca gcgtttgcaa ggtggccccg gcgggccggc
     8521 tttgtgcata cggctgtagc agaaaagcca gcagccgctc ggcaaagatg aagctgaaga
     8581 tcgtcgtcac caggacggcg atgccggcgc gcatcacgtg cacgcgcagc tcgttgaggt
     8641 ggtccagaat ggacatgacc ggcccttcgg catcgggccg gttgcggtca cggcgctcgt
     8701 cggttgtcat ggctaatccg ggtcgtcggt gatgtccacg cgacgcggaa gcgccgggga
     8761 aagggcgggc ggcgtgccgg tggccggggg tgccgaggga gtcgcttcgg ggaagagact
     8821 gggcggtcga tggacggccg ggccgggcgt ggccgcgctg attggcgtgg gcgcggtcgt
     8881 cgccctggct tcgcgcgtcg ccgccaacaa gctgggcggc atgatggcgt tctgggcctg
     8941 cttcaactcg cgcatgacct gcttgccctc ggcggccgtg ccgctggcaa tggtcagcac
     9001 ttcggcgcgc aactcggcca cctgtcgccg cagcagttgt agctcgtcaa tggtctcgcg
     9061 cagttcgcca ctgctgtcgg ccgcgtctag ttcagccgtt agctggtgca cgaaggcgcg
     9121 cgacaccgac tggaggcgcg cggtcacgac gcccaacatc cgcgcatagc ggacaatgcg
     9181 ctctgggccc atgaccatgc cggccaaaac aaccaccagg atcagttccg gcaggccgat
     9241 tccgaagaca ctatccatcg gcgcgaatta cgacttgggc agggtgtcgc cggttccctc
     9301 tggctttttc tccgcggctt ccttttcttc ctggccttcc ttcaggccct ggcgaaaggc
     9361 cgagacgccc ttgcccaact caccgccgac gcgggcgata cggccgacgc cgaagatgag
     9421 tagcacaatc accaaaatga tgatccattc ccaaccacct ggtaacatat cacgttctcc
     9481 ctgtctctcg acaaaccatc tactcgaata gcattatatc attgcctatc tgtcatcgta
     9541 gcttgcgctt agccactata tagcgattag ccgccggccg gggttgccgc cgggcagttg
     9601 accggcactt cacgatagct gaggaaccag gccggcaagg tcgcgaaccg atcgcgcggc
     9661 ccctggagac ggccgatctc cgccgcctgc acagcgtcgc taagaacgta gccggtcaca
     9721 tgttggtaga gcgtcaacgc gggcaggtcg gcgtcgaact ggcgcaaaaa ggcctcatag
     9781 tagacggcgc gctcggcctg gggcataagc tgtcgcgccg cttccagtgc ctcgctggcc
     9841 cggcgattgt tccagcggcc gtagttgtgg ccgcgcacga tggcttcctg gctccagaag
     9901 tcatacaggt cggggtcggc cgggggaacc acgtcgagca gggccacgtc gaaatcgccg
     9961 ctgtcgagag cggcgcgcaa gctgtcggcg tcgggcaaca gcgtcacggg cgtgtcgatg
    10021 cccagcgccg cccactgccg agcgatctcc tgggccacgg cccgcggctc cggctggtca
    10081 accgccacca gacgtaacgt gagcagcccg ccgtcgtgga agcgcccgcc gtcgccgaag
    10141 ggccacccgg ctgtgtcgag cagcgccgcg gccgtctccg gctggtagct ataggccgtc
    10201 atcagccccg gccgcgccgc ccaggaatcg ggcaggtatg gcccctcgaa gaccaacccc
    10261 tgcccattga gcacggtggc gatgagcgcc gcccggtcaa ggccataggc caacgcctgg
    10321 cggacttcct tgctacgcgc cgccggcgtg cccgtgtcgc ccaggttgaa gaacagggcc
    10381 gtgtagcgct gggccggcga ggaaaacagg cgcaccccct cggccgtcag tacatccggg
    10441 gccaatgtcg gcggtaggtt gttggcggca tggatctcgc cgttgtggaa ggcggttagc
    10501 aaggcatcgg ccgtggcgaa aaagcggaac tcgatgtcgc tcagcagcgt accctcacgc
    10561 cagtcgatgg ggttaggcgt caggcgcaga cggccgctct gttgccagtc ctgaccgggc
    10621 tgaaccatga acggccccgt gccaatgggg gcggcgttga aagcgatgcc gtccaggtcg
    10681 gccggcttga tgtcgcccag cagatgggcg ggcaggatgc cgcgcgtcgt cgcctcgatg
    10741 aaaggtgcat agggctgggc cagcgtgaac tggatgtggt ggtcgtcgat gacggtgatc
    10801 gtcgccgact gccacagagg gatcaactcg gccggcccgg ggaagtcgtc ggactgcatc
    10861 aggccgtagg taaaggccac gtcctcggcc gtcaccggct cgccg
//
LOCUS       DAOSMQ010000038        10859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6037_length_10859_cov_3.980563, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000038 DAOSMQ010000000
VERSION     DAOSMQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6037_length_10859_cov_3.980563"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PK829_02290"
     CDS             <1..584
                     /locus_tag="PK829_02290"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU40075.1"
                     /translation="TNTLQPLAPLPTAAATTPATATPAPGGGGGFLGGDGVAPLLLGG
                     LLLLAGLSILGLLMLRRRRPARPARAAVRAATVPLPIGTEEVTGVLAPVYLELESDSS
                     QRFAIHRTPFAIGRDPGNQLVIDESFPQWQTVSREHAIISRHERGYLIEDLGSQNGVR
                     VNGRLTPKNLLRTGWQVTIGGIAFRFVDETQTN"
     gene            596..2305
                     /locus_tag="PK829_02295"
     CDS             596..2305
                     /locus_tag="PK829_02295"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOU40076.1"
                     /translation="MDDILCPNCQTPNRPGAKFCTSCGLNLVEKPAPTTLVMDEATLR
                     HGGARPTVPLPGSRLTDISPRPPARASNGTPTTPLGPPPAAEPLPEGALLADRYLIGQ
                     QVQGPPGVFVYQAEDSDGKPPYGEACLIKESADEAALAGELAAMRAIPPGDGVRPPYD
                     AFTLMLRAASGPRTFVVGSRGRPFEAMEWPVETALALAQAAPLARGLAAVHAAGLAFG
                     GLAGRVWSDGELFLGDFAGAAAGTTATYDADVRALAGMIDRMFTGQTEATLGVTTPLG
                     DHLAGLMAGRITMSAAELAALLSSRAAGLRRPESFDLRVGRRTDVGQLRELNEDSLLT
                     LDLVWSNKSVSRPVGLFVVADGMGGHEGGEVASGLLVRAVAQHAASELMPRTTAAGGE
                     PLDFGAWLTAAIQSGNVEVYERSQRVGNDMGTTVVAALVAGDEAYIAHVGDSRAYRIN
                     SDGIQQITVDHSLVESLVASNQITREEARHHPQSNVIYRTIGDKRQVTVDLDRLTLAP
                     GDALLLCSDGLSGMLTDETIHRLVMSATSPQAACDALIEAANRAGGEDNVTAIVVQTE
                     ALS"
     gene            2329..3054
                     /locus_tag="PK829_02300"
     CDS             2329..3054
                     /locus_tag="PK829_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40077.1"
                     /translation="MLDLWGETTTDVGAPPQGAIFYPGAGRDPLSDEWIAARGYLLAV
                     VGGAGRAAARDFIAALVPAYYASRGGAGGLRQAVEVAEADTAAAGPLAYVVAALADGN
                     LFLARMGGGRAYRVRGATVESLARATAPVGPQPAYSLAVPLLPGDRVALCSAAVAAAV
                     GDGRGLAYVAEQRSPREAASRLVALAHEHGAGGDVSVVVAFVRPAARFNPAQMRVLLA
                     LGVAALAALGWLVWELWRLGQAA"
     gene            3088..4203
                     /locus_tag="PK829_02305"
     CDS             3088..4203
                     /locus_tag="PK829_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU40078.1"
                     /translation="MDEKDPLQAYTAQIVALGQAALAHYRRGESLPEALGGLAAALLA
                     LEDELRPPTTKAAPADTASAVDPTVGPTPAEDESPAGAGQMAPLSASPALVDDWLPAG
                     VGLTEAIPADQPVAAELPLPAGLAPPGPTAVFGDDNAPETPGEVDLAAMTPVEDNDWL
                     TQFAEESSGMLVIREDETSETPGEQPLVVAVEEEAGDPAVVASPMEALPPLVSAPVSL
                     PADEAAWPGTATEPAAVPAAGPPAAPELAASELTTELKYCTNCGAPLRPGRRFCYRCG
                     ASVAEMAAEVGAGAPPAAEPALPPVPARPPVPEPPPASGPSSEWPTIVGDVPNFEDVA
                     TAPPASLPALARFCNNCGLGVAAGVTVCPECGGRDFT"
     gene            4338..5588
                     /locus_tag="PK829_02310"
     CDS             4338..5588
                     /locus_tag="PK829_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU40079.1"
                     /translation="MAGEVTITASLNRPRFATLETEQMAYVYLEARPAEGATTAQAPL
                     NLVLVLDRSGSMAGRKINDLRQAARLTLDRVAPADLVSIVVFDDTVAVLAAARPAADR
                     AALLAQIDRIEERGGTQMSLGLRAGLEQARAGLGPGRVSRLVLLTDGETWDDEDRCRE
                     LAQTAAQMGVPITALGLGDEWNQALLTDLAELSGGNWEFIDAPDRILEAFQQVVVAMQ
                     RTLVTNATLGLRLVKGVRAVAAWRVQPLIDRLSARAVGERDVQVTLGDLQSEGQSVLI
                     ELAVPPRAPGNYRLAQAEVNYDIPGGGAGRKASADIVFTFVADPAGATAVNGAVMNIV
                     ERVSVFKLMTRALDERDSADAQQRTRRLQAAATRLLDAGEVELAREAEAAAAQLGAGQ
                     TLSPHATKRLVSATRRLDMSDLGS"
     gene            5625..6497
                     /locus_tag="PK829_02315"
     CDS             5625..6497
                     /locus_tag="PK829_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU40080.1"
                     /translation="MSMINCPNCGAQVPAGAAFCDSCGTPLAGVAARPGVLGGSAGPA
                     PAASGSGRCPNCGESVMPGEAFCGNCGTPLSMASPTAFAPPPAAPPASNWSPPAPASS
                     PPPTQVARDWIEPEAMPATDSVCPNCGAPVQPADVFCDNCGHALTPRSGFPAGQQAPA
                     LFTSDPSLPLVSTPPGAMGVPVLSPRLVVPSSGATLNLAPGKTEYLIGREDPVSGSFP
                     DVDLVPYGGEEGGVSRRHAQIASRGGQFTIEDLNSVNYTFVNRQKVAPGTPHPLRDGD
                     EIRLGRVVLQFKTS"
     gene            6530..7555
                     /locus_tag="PK829_02320"
     CDS             6530..7555
                     /locus_tag="PK829_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40081.1"
                     /translation="MTTDSGTGRRLPEPAVCPNGHVNRPGTRICAVCRALIPPAATTA
                     APVAPPPAGPAPAAPGPARVANRALGLMLLLLALAVAALAVYYPVRRPAGSLPTTSIT
                     PPAATPLSTIVGIVLTPTLDVATLALAPGAGVPGPNLIHNGDFNADWSDGWERTAEGV
                     TGVQTVATLPSTTPTADGAPPTPRLAMSKSGAGRVQLSQTIPLAQSPVGLVFRGQLRL
                     SGVADGTGEGRSALMLRYEDSNGRLLGASVWLDGSATTSALWGHALPPFGPGVAPRSQ
                     TAAWQTIEVALAQEFADRLPDLDPEAVRRVVVVLLLAGTDDCPPDACVAELSAAALSL
                     VPGELPR"
     gene            7552..8469
                     /locus_tag="PK829_02325"
     CDS             7552..8469
                     /locus_tag="PK829_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40082.1"
                     /translation="MNGRGQTGPGFCHLCGRPLNARARRFHHAAWPPELLLTVCEVCL
                     AEKPRCPVCRLPGNAAGACPTCREVGPICAGCGRALGRQATTFGQSGPYCQECAAGPR
                     CQSCGLPAGRRATAIDGRLLCTVCGATTVVSDDEAAAVYRAAESILREQLGLTLNVPT
                     GLALVTPDELADILGDMGEDSHSGVETPGVETLGVETLGVYTRRGRRRGIYAVRGLPR
                     RLLLQVAAHELAHAWQMENAPLLGDPLLREGFAEWVAYRVLLAVGEDEAAERILARQD
                     NYGQGARLLLDMEKQVGARGVLDWSRRAR"
     gene            8512..9315
                     /locus_tag="PK829_02330"
     CDS             8512..9315
                     /locus_tag="PK829_02330"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU40083.1"
                     /translation="MRYAVRRVSPLSALLYGLLLGLAGWLVPGVLLGLLARAAVERLL
                     LWLASLSFVLPLPLTDGVAIDLVPVLQLSDAQTRMAALSAREAGLVLVVALATAAAGM
                     LLTGLSALLGALIYNLLARGFGGVEVTLDPLEATAAPAPVTRPASATPAPRAAAAPAA
                     PAVPPPVARPSRPTPHAWLVPTKGGGDWLPLGDDVTRIGSAPDNDIVLAGLSPQHAEI
                     RRESGRYLLYDLGSRRTWVNDVQVAAVHMLKDGFRVQLGQAEFMVQMVA"
     gene            9323..10153
                     /locus_tag="PK829_02335"
     CDS             9323..10153
                     /locus_tag="PK829_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOU40084.1"
                     /translation="MDADTIWQLYPTPAREAPLNGLYLSHALRDIGAASGRPFVYTNY
                     VQSIDGRIAIPRPSGQGMMVPTDTSNDRDWRLFQELAVQADCLISSGRYLRDYARGKA
                     QEILRVYDEPRFEDLGRWRAERGLPRYPDLAVISGGLDFPIPSFLTEAGRKVIVITDG
                     AAEGERMARLRDEGATVVVAGQKDVGGRAAIDALGERGYRTLYMATGPRVHHLLLADH
                     VLDRLYTTVAHRLLGGQPFSSMVEGDLLRPAVGMRLHALYYDPHALDGLGQQFVCYAR
                     "
     gene            10153..10854
                     /locus_tag="PK829_02340"
     CDS             10153..10854
                     /locus_tag="PK829_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU40085.1"
                     /translation="MAIALVVFVAAAVQGVMGFGGALVAMPLLVTLVGVQTATPAFAI
                     IGTVATLLNAIRWRAHVTLGDLAQLVVPAALGIPIGVFFLARVDASIVTRALGVILIV
                     YTAYNLAGWSVPPLHGRSWAFAAGFSSGILSGAFNTGGPPVIVYADARGWTAERFRGN
                     LQTYFVLTSVFLLASHALTGHFTPTVWRTALIGLPALLVGQLLGVRLCRYINPDVFRR
                     LVLVFLLLLGARLII"
BASE COUNT         1484 a         3841 c         3820 g         1714 t
ORIGIN      
        1 cgacgaacac gcttcaaccc ctggcgcccc tgcccacagc ggcggccact acccccgcca
       61 ccgccacgcc cgcgcccggc ggtgggggtg gcttcctggg cggcgacggc gtcgcgccct
      121 tgctgctggg tgggctgttg ctgctggccg gcctgtccat cctcggcttg ctgatgttgc
      181 gccgccgccg gccggcgcgc ccggcccgcg ccgctgtccg cgcggccact gtgcccctgc
      241 ccatcgggac ggaagaggtg accggcgtgc tcgcgcccgt ctacctggaa ctggagagcg
      301 attcgtcgca gcgcttcgcc attcacagaa cgccattcgc cattggccgc gaccccggca
      361 accagctggt catcgacgag tccttcccgc agtggcagac cgtctcgcgc gaacacgcca
      421 tcatcagccg gcatgagcgc ggctacctca tcgaagacct cggctcccag aacggcgtgc
      481 gcgttaacgg tcgcctgacc cccaagaacc tgttgcgcac cggctggcaa gtgaccatcg
      541 gcggcattgc ctttcgcttt gtagacgaga cccagacgaa ctaaacagta ggcccatgga
      601 cgatatcctc tgcccgaatt gtcagacgcc gaatcgcccc ggggcgaagt tctgcacgtc
      661 gtgcggcctg aacctcgtgg agaaacccgc gccgacgacg ctggtcatgg acgaggcgac
      721 gctacgccac ggcggcgctc ggccgacggt gccgctgccc ggttcccgcc tgaccgacat
      781 ttcgccgcgc ccgccggcac gggcgagcaa cggcacgccc acgacgccgc tcggtccgcc
      841 gcccgcggcc gagcctttgc ccgaaggggc gctgctggcc gaccgctacc tcatcgggca
      901 gcaggtgcag gggccacccg gtgtctttgt ctaccaggct gaagacagcg acggcaagcc
      961 tccctacggc gaggcctgcc tgatcaagga gagcgctgac gaggcggccc tggcgggcga
     1021 actggcggcc atgcgcgcca ttccgccggg cgacggcgtg cgcccgccct acgacgcctt
     1081 cacgctgatg ctgcgcgccg ccagcggccc gcgcaccttc gtcgtgggca gccgcggccg
     1141 gccgttcgag gcgatggagt ggccggtgga aacggcgctg gcgctggccc aggccgcgcc
     1201 gctggcccgc gggttggccg ccgtccacgc cgccgggctg gccttcggcg ggctggcggg
     1261 gcgcgtgtgg agcgatggcg agctattcct gggcgacttc gccggagcgg cggccgggac
     1321 cacagccacc tatgacgctg acgtgcgcgc cctggccggc atgatcgacc gcatgttcac
     1381 tggtcagacg gaagcgactc tcggcgtgac cacccctttg ggcgaccatc tggccgggct
     1441 gatggccgga cgtatcacga tgagcgcggc cgagctggcc gcgttgcttt cctctcgcgc
     1501 cgcgggcctg cgccgtccgg agagcttcga cctgcgcgtc ggccggcgca ccgacgtcgg
     1561 ccagttgcgt gaattgaacg aggatagcct gctgacgctc gacctcgttt ggagcaacaa
     1621 gtcagtcagc cggccggtgg gcctgttcgt cgtcgccgac ggcatgggcg gccacgaggg
     1681 cggcgaagtg gccagcggcc tgctggtgcg cgccgtggcc cagcacgcgg ccagcgaact
     1741 gatgccgcgc acgacggcgg ccggcggcga accgctggat ttcggcgcct ggctgacggc
     1801 ggcgatccag tccggcaacg ttgaggtcta cgagcgctcc cagcgggtgg gcaatgacat
     1861 gggtacgacc gtcgtcgccg cgctggtggc cggcgacgag gcttacatcg cccacgtcgg
     1921 cgacagccgc gcctaccgca tcaatagcga tggcatccaa cagatcaccg tcgatcattc
     1981 cctggttgag agcctggtcg cctccaacca gatcacccgc gaggaagccc gccaccaccc
     2041 gcagtcgaac gtcatctatc gcactatcgg cgacaagcgc caggtgacgg tcgatctgga
     2101 tcggctaacg ctggcccctg gcgatgcctt gctgctgtgt tccgacgggc tgagcggcat
     2161 gttgacggac gagacgatcc accggctagt gatgtcggcc acctcacccc aggcggcctg
     2221 cgatgccctg atcgaagctg ccaaccgcgc tggcggcgaa gacaacgtga ccgccatcgt
     2281 cgtccagacg gaggcgcttt cctgagcggc gaaaggccac ggcgcagcgt gcttgacctc
     2341 tggggcgaaa cgacgacgga cgtaggcgcg ccgccacagg gagctatttt ctaccctggg
     2401 gccgggcgcg acccgctgag cgacgaatgg atcgccgcgc gagggtactt gttggccgtc
     2461 gttggcggcg cggggcgtgc ggcggcgcgt gattttatcg ccgcgctcgt gccggcatac
     2521 tatgccagcc gcggtggcgc tggcggcttg cgccaggctg tcgaggtcgc tgaggcggac
     2581 acggccgccg ctggcccgct cgcttacgtc gttgcggccc tggccgacgg caacctcttc
     2641 ctggcccgca tgggcggcgg acgggcctac cgcgtccgcg gggcgacggt ggaatcgctg
     2701 gcccgcgcca ccgcgcccgt cggtccgcag ccggcctatt cgctggccgt gccgctgtta
     2761 cccggcgatc gtgtggcgct gtgctccgcg gcggtcgcgg cggccgtggg cgatggacgg
     2821 ggtttggcct acgtggccga gcagcgctcg ccgcgtgagg cggccagccg gctggtggcc
     2881 ctggcccacg agcacggggc cggcggcgat gtctccgtgg tggtcgcctt tgtgcgcccg
     2941 gcggcgcgtt tcaacccggc gcaaatgcgc gtcttgctgg cgctgggcgt cgccgcgctg
     3001 gccgcgctgg gctggctcgt gtgggaactg tggcgcttgg gtcaggctgc ctagagcggc
     3061 cggccccatg cgtgagggag ttggtttatg gacgagaaag atccgctgca agcctataca
     3121 gcccagatcg tcgctcttgg tcaggcggcg ctggcccact atcgccgcgg cgaatcgctg
     3181 cccgaggcgc tgggcggcct ggcggctgcc ctgctggctc tggaagacga actgcgcccg
     3241 cccacgacca aagccgcccc cgcggacacg gccagcgccg ttgaccccac ggttggcccc
     3301 acgccggccg aagacgagtc gcccgccggc gcgggccaga tggcccccct ctctgccagc
     3361 ccggcgctgg tcgatgactg gctgcccgcc ggcgtgggtc tgactgaggc catccccgcc
     3421 gaccagcccg tggcggccga actgccgctg cccgctggcc tggcccctcc cgggccgacg
     3481 gcggtctttg gcgacgacaa cgcgccggaa acgcccggcg aggtcgattt agccgccatg
     3541 acgccggttg aggacaacga ttggctaacc cagttcgccg aggaatcgag cggcatgctg
     3601 gtgatccgcg aggacgaaac gagcgagacc cccggcgagc aaccgctggt cgtcgctgtc
     3661 gaggaagagg ccggcgaccc ggcggtggtt gcttcgccga tggaggcgtt gccgccgctg
     3721 gtgagcgccc cggtcagtct accggccgac gaggcggcat ggccgggcac agccaccgaa
     3781 ccggcggccg tgcccgcggc cgggccgccg gccgccccag aactggcggc ctccgaactg
     3841 acgaccgaac tgaagtattg caccaattgt ggcgcgccgc tgcggcccgg ccggcgcttc
     3901 tgctatcgct gcggcgcgtc ggtggccgag atggcggccg aggtgggcgc gggcgcgcca
     3961 ccggccgcgg agccggcgct gccgcccgtc ccagcgcggc cgcctgttcc ggagccgccc
     4021 ccggccagcg gcccgtcgtc ggagtggccg accatcgtcg gtgacgtgcc caactttgag
     4081 gatgtcgcca ccgcgccgcc ggcaagcctc cccgctctgg ctcgcttctg caataactgc
     4141 ggcctgggtg tggccgccgg cgtgacggtc tgcccggaat gtggcggccg cgactttacc
     4201 tgaggccctg gacattcgga accgtggccg cccacctgtc aggttggtgc gccgtgggtg
     4261 actttggcaa agccctgcct agcggccgtg aataccagtc gaagcgggct ataatcgggc
     4321 agcatggagg tgtaagcatg gcgggcgaag tgacgatcac agcctcactc aaccggccgc
     4381 ggttcgccac cctggaaacg gagcagatgg cctacgtcta cctggaggcc cgcccggccg
     4441 agggcgcgac cacggcgcag gccccgctca accttgtcct ggtgctcgac cgcagtggct
     4501 cgatggccgg ccgcaagatc aatgacttgc gccaggccgc ccggctgacg ctcgaccgcg
     4561 ttgcgcccgc tgacctggtg tccatcgttg tcttcgacga cacggtcgct gtgctggccg
     4621 cggcccgccc ggccgccgac cgcgctgccc tgctggccca gatcgaccgc atcgaggagc
     4681 gcggcggcac gcaaatgtcg ctggggctgc gggccgggct ggagcaggcc cgcgccgggt
     4741 tggggcccgg ccgcgtcagt cgccttgttc tgctgaccga tggcgagacg tgggacgatg
     4801 aagaccgctg tcgcgagttg gcgcagacgg cggcgcagat gggcgtgccc atcaccgccc
     4861 tgggcctggg cgacgagtgg aatcaggcct tattgaccga cctggccgag ctgtccggtg
     4921 gtaactggga gttcatcgac gcgcccgacc gcatcctgga ggcattccaa caggtggtcg
     4981 tcgccatgca gcgcaccctg gtcaccaatg ccacgctggg cctgcggctg gtgaagggcg
     5041 tgcgcgcggt ggccgcctgg cgcgtccagc cgctgatcga ccgcctctcg gcgcgggccg
     5101 tgggtgagcg tgacgtgcag gtgaccctgg gcgacttaca aagcgagggc cagagcgtgc
     5161 taatcgaact ggccgttccg ccgcgcgccc cggggaatta tcggctggcg caggctgagg
     5221 taaactatga catccccggc ggcggcgcgg gccgcaaggc gtcggccgac atcgttttta
     5281 cctttgtggc cgacccggcc ggcgcgacgg ccgtcaacgg cgcggtgatg aacatcgtcg
     5341 agcgggtcag cgttttcaag ctgatgaccc gcgccctgga cgaacgcgac agcgccgacg
     5401 cccaacagcg cacccggcgc ttgcaggccg cggcgacgcg cctgctggac gcgggcgagg
     5461 tagagttggc gcgcgaagcg gaggcggccg cggcgcagct tggcgcaggg cagaccctct
     5521 cgccccacgc caccaaacga ctggtctccg ccacgcggcg gctggatatg agcgacctgg
     5581 gcagctgaca aggcgtcaca ggcgcacagg atgggaagga tgagatgagc atgatcaact
     5641 gccccaattg tggcgcgcag gttccggccg gcgcagcctt ctgtgacagc tgcggcacgc
     5701 ccctggctgg cgtggccgct cgccccggcg tgttgggtgg ctcggccggc cccgctccgg
     5761 cggcctctgg ctcggggcgc tgtcccaact gtggcgagag cgtgatgccc ggcgaggcgt
     5821 tttgcggcaa ttgcggcacg ccgctcagca tggcctcgcc gacggccttc gcgccgccgc
     5881 cggccgcgcc accagcctcg aattggtcac cgcccgcccc ggcgtcctcg ccaccgccga
     5941 cacaggttgc tcgcgactgg atagagccgg aggcgatgcc cgccacggat agcgtctgcc
     6001 ccaactgcgg cgcgcctgtc caaccggccg atgtcttttg cgacaactgt ggccacgcgc
     6061 tgacacccag gtctggcttt cctgccggcc agcaggcccc ggccctgttc accagcgatc
     6121 catcgctgcc tctcgtctcg acgccgcccg gcgccatggg cgtgccagtg ctcagcccgc
     6181 gcctggtggt gccgtcgtcc ggggcgacgc tcaatctggc cccgggcaag acggaatacc
     6241 tgatcggccg tgaagacccg gtcagcggca gcttccccga cgtcgatctc gttccctatg
     6301 gtggcgaaga gggtggcgta tcgcgccgcc acgcccagat cgccagccgt ggcggccaat
     6361 tcaccattga ggacctgaac tcggtcaact acaccttcgt caaccgccag aaggtggccc
     6421 ccggcacgcc ccacccgctg cgcgacggcg acgaaattcg actgggccgg gtcgtgttac
     6481 agttcaagac gagctaggcg cgggcgtcgg ccggcgctga tgagcgttaa tgaccacgga
     6541 ctcaggcacg ggacgacgcc tgccagagcc ggccgtgtgc cccaacggcc acgtcaaccg
     6601 gcctggcacg cgcatctgcg cggtctgtcg ggcgctcatc ccgccggcgg ccacaacagc
     6661 tgcgcctgtc gccccaccgc cggccgggcc ggcgccggcg gcgcccgggc cggcccgggt
     6721 tgccaaccgg gcgctggggc tgatgctgct tctgctggcg ctggccgtgg ccgcgttggc
     6781 cgtctattat ccagttcgtc gcccggctgg ctccctgcca acgaccagca taactccacc
     6841 ggccgctacg ccattatcga ccatcgtcgg catcgtcctg accccgacgc tggatgtggc
     6901 cacacttgct ctggctccag gggcgggtgt ccctggcccc aacctgatcc acaatggcga
     6961 tttcaacgcc gactggagcg acggctggga gcgcacggcc gagggcgtga cgggcgttca
     7021 gactgtggcg acgttgcctt cgacgacacc gacggcggac ggcgcgccac cgacgccccg
     7081 gctggctatg agtaagtcgg gtgctgggcg cgtgcagctt agccagacca tcccgctggc
     7141 ccagtcgccg gtcgggctgg tgtttcgtgg gcaactgcgg ctgtccggcg tcgccgatgg
     7201 gaccggtgag gggcgtagcg cgctgatgct gcgctatgag gacagcaacg gccggcttct
     7261 gggcgcgtcg gtgtggctcg acggcagcgc gaccacgtcg gccctgtggg gccatgccct
     7321 gccaccgttc ggccccggcg tggccccgcg cagccaaacg gctgcgtggc agacgatcga
     7381 ggtcgcgctg gcccaggagt tcgccgaccg cttgcccgac ctcgatccgg aggcggtgcg
     7441 ccgcgtcgtc gtcgtcctgc tgctggccgg cacagacgac tgcccaccgg atgcctgtgt
     7501 ggcggagttg agcgccgcgg cgctgtcgct tgtgcccggc gagctgccgc gatgaacggc
     7561 cggggccaga cggggccagg cttctgccac ctctgcggtc ggccgctgaa tgcgcgcgct
     7621 cgccgcttcc accacgccgc ctggccgccg gaactgctcc tgaccgtctg tgaggtctgc
     7681 ctggccgaga agccgcgctg tcccgtttgc cggctgcccg gcaacgccgc cggggcatgc
     7741 cctacgtgcc gcgaggttgg gccgatctgt gccggttgtg gccgtgcgct gggccgccag
     7801 gcgacgacct tcggccaaag cgggccatac tgtcaggagt gcgccgccgg gccgcgctgc
     7861 cagagttgtg gtctgccggc cggacgccgc gcgacggcca tcgacggccg gctcctgtgt
     7921 acggtctgcg gagccacgac cgtggtcagc gacgacgagg ccgccgccgt ctatcgcgcc
     7981 gctgagagca tcctgcgcga gcaactgggc ctgacgctca acgtgcccac ggggctggcg
     8041 ctggtcacgc ccgacgagct ggccgacatc ctgggcgaca tgggcgaaga ctcccacagc
     8101 ggcgtcgaaa cgccgggcgt tgaaacgctg ggcgttgaaa cgctgggcgt ttatacgcgg
     8161 cggggacggc ggcggggcat ctacgccgtc cgggggctgc cgcggcggct gctgcttcag
     8221 gtggccgcgc atgaattggc ccacgcctgg cagatggaaa acgcgccgct gctgggcgat
     8281 ccgctgctgc gcgagggctt tgccgagtgg gtggcctacc gcgtcttgct ggctgtgggc
     8341 gaggatgagg ccgccgagcg catcctggct cgccaggaca actacggcca gggagcgcgc
     8401 ctgctgctgg acatggagaa gcaagtcggc gcgcgcggcg tcctcgattg gtctcgccgg
     8461 gcgcgctgac cagcatgtgc cctggaaaca gggatcaggt actatagagg gatgcgttac
     8521 gctgtccgtc gcgtcagtcc gctatcggct ctgttatatg gcctgctgct cggcctggcc
     8581 ggctggctgg ttccgggagt gctgctaggc ttgctggcgc gcgcggccgt cgaacggctg
     8641 ctcctctggc tggcgtcgct cagctttgtg ctgcctctgc ccctgaccga cggcgtggct
     8701 attgaccttg tgcccgtgct gcaactgagc gacgcgcaga cgcgcatggc cgctctgagc
     8761 gcgcgcgagg cgggcctggt gctcgtcgtc gcgctggcca cggccgccgc gggcatgttg
     8821 ctgaccgggc tgtcggccct gctcggcgcg ctcatctata acctcctggc gcgaggcttt
     8881 ggcggcgtgg aagtaacact cgatcccctg gaagccaccg ccgcccccgc ccctgtgact
     8941 cgcccggcga gcgcaacgcc agccccccgg gccgccgccg cgcccgctgc cccggccgtg
     9001 ccgccgcccg tggcccgccc cagccggcca acgccccacg cctggctcgt gccaacaaag
     9061 ggtggcggcg actggctgcc gctgggcgac gacgtgaccc gcatcggcag cgcgccggac
     9121 aacgacatcg tcctggccgg cctctcgccc cagcacgccg agattcgccg cgaaagtggc
     9181 cgctacctgc tctatgacct gggcagccgc cgcacatggg tcaacgacgt ccaggtggcc
     9241 gccgtccaca tgctaaagga cggcttccgg gtgcagttgg gccaggccga gttcatggtg
     9301 cagatggttg cctgagatgc atatggacgc cgacaccatc tggcaactct acccgacgcc
     9361 ggcgcgcgaa gcgccgctta acggcctgta cctgagccat gccctgcgcg acatcggcgc
     9421 ggccagcggc cggccgtttg tctacaccaa ctacgtccag agcatcgacg gccgtatcgc
     9481 catcccccgg ccgtcgggcc agggcatgat ggtgccaacc gatacgtcca acgaccgtga
     9541 ttggcggctg ttccaggagc tggctgtgca ggccgactgc ctgatcagca gcggccgcta
     9601 tctgcgcgac tacgccaggg gcaaggccca ggagattctg cgcgtctacg acgagccgcg
     9661 cttcgaggat ttgggccgct ggcgggccga gcgcggcttg ccccgctacc ctgacctggc
     9721 cgtcatcagc ggcggcctcg actttcccat cccatcattc ctgaccgaag ccggccgcaa
     9781 ggtgatcgtt atcaccgacg gcgcggccga gggcgagcgc atggcccggc tgcgcgacga
     9841 gggcgcgacg gtggtcgtcg ccgggcaaaa ggacgttggc ggtcgggcgg ccattgacgc
     9901 gctgggcgag cggggctacc ggacgctcta tatggccacc ggcccgcgcg tccaccattt
     9961 gctgctggcc gaccacgtgc tcgaccgcct ctatacgacc gtggcccaca ggctgctggg
    10021 cggacagccc ttcagcagca tggtggaggg agatttgctg cggccggctg tcggcatgcg
    10081 cctgcacgcg ctgtattacg acccgcacgc cctcgacggg ctggggcagc agttcgtctg
    10141 ctacgcgcgc tgatggctat cgcgctggtg gtcttcgtcg ccgcggccgt ccagggcgtc
    10201 atgggctttg gcggcgcgct ggtggccatg cccctgctgg tgacactcgt gggcgtgcag
    10261 acggccaccc cagccttcgc catcatcggc accgtggcga cgctgctcaa cgccatccgc
    10321 tggcgggcgc atgtgaccct gggcgatctg gcccaactgg tggttccggc ggcgctgggc
    10381 attcccatcg gcgtgttttt cctggcgcgg gtggacgcca gcattgtcac gcgagcgctg
    10441 ggtgtcattc tgatcgtcta caccgcttac aacctggctg gctggtctgt gccgcccctc
    10501 cacggccgca gttgggcctt cgccgccggt tttagttcgg gcatcctcag cggggcgttc
    10561 aacacgggtg ggccaccggt gatcgtctac gccgacgccc gcggctggac ggccgagcgc
    10621 ttccgcggca acctccaaac ctacttcgtg ctgaccagcg tctttctgct ggcctcccac
    10681 gcgctgacgg ggcacttcac gcccacggtg tggcggacgg ctctcattgg cttgccggcg
    10741 ctgctggttg ggcagctatt gggcgtgcgc ctgtgccgct atatcaaccc cgacgtgttc
    10801 cggcggctgg tgctggtttt ccttctcctt ctgggggcgc ggctgatcat ctgaccggt
//
LOCUS       DAOSMQ010000039        10843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6052_length_10843_cov_4.344457, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000039 DAOSMQ010000000
VERSION     DAOSMQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6052_length_10843_cov_4.344457"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PK829_02345"
     CDS             complement(<1..808)
                     /locus_tag="PK829_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOU40086.1"
                     /translation="MGLAGSLGLGGLARAGAPTAGHSQPAAQSGTPATDDHLAAHGGM
                     GTVGQVDHAANQFDPHEILTDFDTGELSEENGRPVRTWQMFAVDREFEIAPGVHFPGW
                     VYGTPTSAASRRAGHVVGQCPGPTLRCVEGERLRIHFTNASSHPHTIHFHGIHAAAMD
                     GIPGVGVGMIAPGGQFTYEFEAKPFGCHLYHCHAVPLKRHIHKGLYGAFIVDPDPAHY
                     TGEEAEAARRRNHTTAESREVDEMVMVLNGFDTNFDGENEVYAANTVAFAY"
     gene            1091..2065
                     /locus_tag="PK829_02350"
     CDS             1091..2065
                     /locus_tag="PK829_02350"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU40087.1"
                     /translation="MSKKALFCSLFLLVLLAACGGQAAPETLRTIRLPMGYVADPQYA
                     PLYVAVERGYFAEEGLALEFDYSFETDGIALVGAGELPFALASGEQAVLARAEGLPVV
                     FVMQWWQQYPIAIVSKAASGIRSPADLAGRQVGVPGFFGASYVGLVGLLFANDMTLDD
                     IDANEIGFNQVESLLTGQSEAVVVYINNEPVQLAQRGEALNVIAVSDYVNLVANGLMT
                     NEQTIAEEPELVQGFVRAFLRGLRDTLDDPDAAFEISKRYVEGLDDSRRGVLDASIDL
                     WQADQLGRGDPAAWQETHDLLLAMGLLETPLPDVGALYTNDFVDQAQP"
     gene            2072..2893
                     /locus_tag="PK829_02355"
     CDS             2072..2893
                     /locus_tag="PK829_02355"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40088.1"
                     /translation="MTADFLTLRDVGLIYSDNNGRRRDHVLAGVSLDIPIGAFVALVG
                     TSGVGKSTLLRVIAGLLPPDGGSVRLLGSDPRTVEAPIGIVFQRDNLMPWRTVAENVR
                     LPLEMHAGPGQSLGRVGALPPSDGATALDTSAQVLDALALVGLADQAGSYPAQLSGGM
                     AQRVALARALVHHPALLLLDEPFGALDALTRERMAQELLRIWQARPVTVLMVTHSIGE
                     AVLLADEVLVMNGRPATITDRFVIDLPRPRAPELTDTAAFQATVAAVRAAIRVKD"
     gene            3101..3652
                     /locus_tag="PK829_02360"
     CDS             3101..3652
                     /locus_tag="PK829_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40089.1"
                     /translation="MPWRKGVAWWVVFLEGVTLLGLGLVMFFARETTHLFLGWIIALA
                     LTASGALSLWLALNAIKRDEVRLWTMIHGAIGLAGGLLVILLLLFGSFLEGPGLTILG
                     LGCLAYGGVGLYLLADKRLSSLRRLSFISTALYLALGVLILLQVMGVGVLATKLQIIN
                     LIVVIAGIILIFWGLILRNERAQ"
     gene            3693..4277
                     /locus_tag="PK829_02365"
     CDS             3693..4277
                     /locus_tag="PK829_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HOU40090.1"
                     /translation="MDSILWGLIVLLCGIFIAAYGHQLFRFALAFIGFALGVYLVMWL
                     GSGLDTALRMVVAIVAGGILASVLYFLVKSALYVAGGIMGLVLMLVVLGLFRLSGLDL
                     GLFGSILALAGAGLGGIFGQRLGPIVTILATSLAGAYFVVLGLGAWFGLGVTTDDPTV
                     TLGSAFPLVLFLTVAAISGLAQHQAFTLRQRFLR"
     gene            complement(4401..5360)
                     /locus_tag="PK829_02370"
     CDS             complement(4401..5360)
                     /locus_tag="PK829_02370"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOU40091.1"
                     /translation="MKRRVGYLVGLLVLALVIVACGGRAYDSAAVTQTASETVVEEAA
                     PQAYDAESDFSMAAGSTVANAPAAEPAQPAQPVADQERLIIRNADMSIVATDTEAALA
                     QIAAMATNGGGWVVSSNVYQSSETSKTGYIQIRVPSEGFQSVLDAIAGLAVSVESLST
                     SGQDVTEEYVDLSSQLTNLEATAARVRTFLDDATRVEDALAVNAELSRLEGEIAVTKG
                     RMQYLSQSAAFSSITVNVTPDELAQPIEVAGWQPRGVAKEALESLINALQSLANLVIW
                     LVVFVLPVLLVVAIPLLIIFLLIRWLRRRQGRVATPATPPVEK"
     gene            complement(5445..6176)
                     /locus_tag="PK829_02375"
     CDS             complement(5445..6176)
                     /locus_tag="PK829_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU40092.1"
                     /translation="MRVLLLSDIHANLVAFEAVLAEAEGQWERLWFLGDLVGYGPQPN
                     ECVERLRALEPLALSGNHDWAVLDKLDDEDFNSDARYVARWTRRGLTADNRAYLNTLP
                     PLRVEAPFTLAHASPRHPVWEYILDLPTALENFAHFDTPCCLVGHTHVPAVFALDEKT
                     GGVDFALIEHGDVLDLSRHRLIVNPGGVGQPRDGDPRAAYALLDTEAMRMTFHRVAYD
                     IAATQVLMREQKLPRRLIKRLEQGL"
     gene            6426..6926
                     /gene="tsaE"
                     /locus_tag="PK829_02380"
     CDS             6426..6926
                     /gene="tsaE"
                     /locus_tag="PK829_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU40093.1"
                     /translation="MAILDDRTLDFVSTSPAQTERLGIRLGELLEPGDIVCLAGELGA
                     GKTTLARGIGRGWGTPLRVTSPTFILVNEYPRPRDGATLYHLDCYRLERAGDIATVGL
                     EDLFDSDATVMIEWPEHILALLPADRLWIALSYDSPTRRRLRLNAGGPRATDLLADFR
                     KNAFGV"
     gene            6933..7592
                     /gene="tsaB"
                     /locus_tag="PK829_02385"
     CDS             6933..7592
                     /gene="tsaB"
                     /locus_tag="PK829_02385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOU40094.1"
                     /translation="MILAIDTATDWIGLALHNGAAILAEFGWRSRRTQTIELAPAVAQ
                     LWGRAGVAPTDLNAVAVAIGPGSYTGLRVGLALAKGLALAHNLPLVGVPTLDIVARSV
                     CRLETDLVVVAQAGRTRVWAGQYQWVAKRGWERVGEPLLTNWEDLAARLALPVAFAGE
                     IDAAAAKLIRRTNRAAAIAPPAESVRRAAMLAEIGWQRWKRKQTDPAGSLAPLYLREP
                     G"
     gene            7598..8131
                     /gene="rimI"
                     /locus_tag="PK829_02390"
     CDS             7598..8131
                     /gene="rimI"
                     /locus_tag="PK829_02390"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOU40095.1"
                     /translation="MLALPPPYRLRPMTVADIPAVLAVDRASFPTPATEQLFVNELTD
                     NQLAHYQVLLRDAAGGETLVGFAGFWLIAGEIHISTIAVSPAERGRGRGEWLLLNLLL
                     LACALEPLLITLEVRRSNTVAQTLYAKYRFVEVGLRRRYYRDPPEDALLMTVDLAEQP
                     DYCVWLAERRPSGRGEE"
     gene            8242..9276
                     /locus_tag="PK829_02395"
     CDS             8242..9276
                     /locus_tag="PK829_02395"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU40096.1"
                     /translation="MSGQHVFITGGAGFIGCNAADHFLGRGDAVTLYDNLTRRGGRAN
                     LDWLHATYGGERLAVVEADIRDYDRLASAVAERPPDVVLHLAGQVAVTQSVADPREDF
                     ESNALGTFNVLEAVRHGAPGAALLYASTNKVYGGMEHVGIVPAGERYAYADYPHGIPE
                     TFPLDFHSPYGCSKGAGDQYMRDYARIYGLRTLVLRQSCIYGRRQFGVEDQGWVAHFV
                     IATVLDRPIAIYGDGRQVRDVLYIDDLLDAYQRGIDHIDDLRGEVLNLGGGPDNTLSI
                     WTEFGSLLAELAGRPVPVRYGPWRPGDQRVFVADIRQAKALLGWQPRVSPAEGVRRLY
                     EWVAANRELF"
     gene            9360..9962
                     /locus_tag="PK829_02400"
     CDS             9360..9962
                     /locus_tag="PK829_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40097.1"
                     /translation="MRHWLRLPLFVLVGVLTAAVGELQYSVLIRGDWANLLGSMVFNA
                     VYLIAAFVVVWALFRLLPRRAAFLACVILAAVAGLGVEWFLIGNSPRGNPDASQLGMA
                     AYWAGMVVVPLIVMDGDARLRPLKRRIAVFAAVYTLAVLLGQWLLPVGDWRFAFHILT
                     VILGYLGLLVLCVVGYLRNARSGSLALLADEPGRLDIQHD"
     gene            complement(9959..10366)
                     /locus_tag="PK829_02405"
     CDS             complement(9959..10366)
                     /locus_tag="PK829_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOU40098.1"
                     /translation="MIVVDANIIAYLLISGDRTPEAQALWRFDNDWVVPDLWRDEFLN
                     ILSTYVRQGGADLETAKKLWGSAVDLFASKERSASPLLVLELAQQYRLSAYDAQYLAV
                     ALELGVMLLTEDRSFHRAVPQFSTTMQPYLQAS"
     gene            complement(10363..10584)
                     /locus_tag="PK829_02410"
     CDS             complement(10363..10584)
                     /locus_tag="PK829_02410"
                     /inference="COORDINATES: protein motif:HMM:NF017333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOU40099.1"
                     /translation="MTDLLIRQLPEDLHNRLKARARANRRSVTQEVLILLEQSLVSGE
                     TYMEPPQPLQGAFDIDDEWLSQARQAGRA"
BASE COUNT         1679 a         3549 c         3584 g         2031 t
ORIGIN      
        1 cataggcgaa ggcgacggtg ttggcggcgt agacctcgtt ctcgccgtcg aagttagtgt
       61 cgaagccgtt gagtaccatg accatctcat cgacctcgcg cgactcggcc gtggtgtggt
      121 tgcggcggcg ggcggcctcg gcctcctcgc cggtgtagtg agccgggtcg ggatcgacga
      181 tgaacgcgcc gtagaggccc ttgtgaatgt ggcgcttcag aggcacggcg tggcagtggt
      241 acaggtggca gccgaacggc ttggcctcga attcataggt gaactgcccg cccggcgcga
      301 tcatgcccac gccgacgccg gggataccat ccatggctgc cgcgtggatg ccgtggaagt
      361 ggatcgtgtg cgggtggctg ctggcgttgg tgaagtggat gcgcaggcgc tcgccctcga
      421 cacagcgcag cgtcggcccg gggcattggc cgacgacgtg gccggcccgg cgcgaggccg
      481 cagaagtggg tgtgccgtag acccagccgg ggaagtggac gccgggggcg atttcgaact
      541 cgcggtcgac ggcgaacatc tgccacgtgc gcacagggcg gccgttttcc tcgctcagtt
      601 cgcctgtgtc gaagtcggtc aggatttcgt gcgggtcgaa ctgattggcg gcgtggtcaa
      661 cctggccgac ggtgcccatg ccaccgtggg cggccagatg gtcgtcagtt gccggcgtgc
      721 cggactgcgc ggcgggctgg ctgtggccgg ccgtcggcgc gccggcccgg gccagcccgc
      781 ccaggccaag gctgccggcc agccccacca gccccccccg ccccgcccgc ccgcagaaat
      841 gttcgtcgtg tgattcccga ttttgcaagc atcttaatgg ttacctgtat acctttgttt
      901 gtcgaaaaga ataaaatatt tcgatgcacc taatatagta actggggcag tggatttgtc
      961 aagataagga gacctgacag gtatacagcc ccaactgccc acctgacagg tctgtgcaat
     1021 cggcgcggct tgtgttattg tctctggccg aatgggccaa acgctcgcct atcgcactca
     1081 agaggaatcc gtgtccaaaa aagccctctt ctgctcgctt ttcttactcg ttctgctggc
     1141 tgcctgcggt ggccaggccg cgccagagac gttgcgcacg atccgcctgc caatgggcta
     1201 cgtggccgac ccacagtacg cgccgctgta cgtcgccgtc gagcgcggct acttcgccga
     1261 agagggactg gcgctggagt ttgactacag cttcgagacc gacggcatcg ccctggtggg
     1321 cgcgggcgag ctgcccttcg ccctggccag cggcgagcag gccgtgttgg cccgcgcgga
     1381 ggggctgccg gtcgtcttcg tcatgcagtg gtggcagcaa taccctatcg ccatcgtcag
     1441 caaggccgcg tcgggcatcc gctccccggc cgatctggcc ggccgccagg tgggcgtgcc
     1501 cggcttcttc ggggccagct acgtcggcct cgtcggcttg ctcttcgcca acgacatgac
     1561 cctcgacgac atcgacgcca acgagatcgg cttcaaccag gtcgaatcgc tgctgaccgg
     1621 ccagtcagag gcggtcgtcg tctacatcaa caacgagccg gtgcagctgg ctcagcgcgg
     1681 cgaggccctt aacgtcatcg ccgtgtccga ctacgtcaac ctggtcgcca acggcctgat
     1741 gaccaacgag cagaccatcg ccgaggagcc ggagcttgtg cagggctttg tgcgtgcttt
     1801 tctgcgcggc ctgcgcgata cgctcgacga ccccgatgcc gccttcgaga ttagcaagcg
     1861 ctacgtcgag gggttggacg acagccggcg cggcgtgctc gatgcctcca tcgacttgtg
     1921 gcaggccgac cagcttggcc gcggcgaccc cgccgcctgg caggaaaccc acgacttgct
     1981 gctggccatg gggctgctgg aaacgccgtt gcccgatgtg ggcgcgctct acaccaacga
     2041 cttcgtcgac caggcccagc cctagcatgg aatgacggcc gactttctga ccctgcgcga
     2101 tgtaggcttg atctacagcg acaacaacgg ccggcggcgc gaccacgtcc tggccggcgt
     2161 gtcgctcgac atccccattg gcgcattcgt ggccctggtg ggcacgtccg gcgtcggcaa
     2221 atcgaccctg ctgcgcgtca tcgccgggct gctcccgccg gacggcggca gcgtccgcct
     2281 gctgggcagt gacccgcgca ccgtggaggc ccccatcggc atcgtcttcc agcgcgacaa
     2341 cctgatgccc tggcgcaccg tggccgagaa cgtccgtctg ccgctggaga tgcatgccgg
     2401 gccggggcaa tcgctggggc gcgtcggcgc gctgccgccg agcgacggcg cgacggcgct
     2461 cgacacctct gcccaggtgt tggacgcgct cgccctcgtc ggcctggccg accaggccgg
     2521 cagctacccg gcgcagctct ccggcggcat ggcccagcgc gtcgccctgg cccgcgccct
     2581 tgtccaccac cccgccctgt tgctgctcga cgagccgttc ggcgcgctcg acgccctgac
     2641 gcgcgagcgc atggcccagg agttgctgcg catctggcag gcgcggccgg tgacggtgct
     2701 gatggtgacc catagcatcg gcgaggcggt gctgctggcc gacgaggtgc tggtgatgaa
     2761 cggccgcccg gcgacgatta ccgaccgctt cgtcatcgac ctgccccggc cgcgcgcgcc
     2821 ggagctgaca gacacggccg ccttccaggc caccgtcgcc gccgtccgcg ccgccatccg
     2881 cgtcaaggat tgaggcgcac acgcccgggg cctttcacgg caatttgtct tatgcgctgg
     2941 gggcctattt attctcttgg tcgctgacta ttgcatgcta tactgactcg ccacgtgacc
     3001 atgctcggcg gcgtcgcgca gtcataatcg ctcaagtcta gtggaggctg ggatgccgaa
     3061 cgaactcaaa tccaaagcgc ttgagacggc cagaggcgct atgccctggc gtaaaggcgt
     3121 cgcctggtgg gttgtgtttc tcgaaggggt caccctgctg gggctggggt tggtgatgtt
     3181 cttcgcgcgc gagacgaccc atctcttcct cggctggatc attgccctgg ctctgaccgc
     3241 cagtggggcg ctcagcctct ggctggctct caacgcgatt aagagggacg aggtgcgcct
     3301 ctggacgatg atccacggcg cgattgggct ggccggtggg ctactggtta tcctgctgct
     3361 gttgtttggc tcgttcctgg aaggccccgg tctgactatt ctagggcttg ggtgtctggc
     3421 ctatggcggc gtagggcttt atcttctggc cgacaagagg ctgtccagcc tgcggcggct
     3481 ctcattcatc agtaccgccc tctatctggc gctgggcgtt ctcatcctgc tccaggtcat
     3541 gggtgtgggc gtgttggcca ccaagttgca gataatcaac ctgatcgtcg tgatcgccgg
     3601 catcatcctc atcttctggg gcctgatctt gaggaacgag cgcgcgcagt agaaccccgg
     3661 tttccgccat tccatcaaca ggagtattca tcatggacag tatcctttgg ggcttgattg
     3721 ttttgctatg tggcatcttc atcgctgcct atggccacca gttgtttcgc tttgccctgg
     3781 ccttcatcgg cttcgctctg ggcgtctacc tggtcatgtg gcttggcagt gggctggaca
     3841 cggccctgcg gatggtcgtc gccatcgttg ccggcggtat cctggcgtcg gtgttgtatt
     3901 ttctggtgaa gtctgctctc tatgtggccg gcggcatcat ggggctggtt ctgatgctgg
     3961 tcgtcctggg cctgttccgg ctgagcggcc tggacctggg tctcttcggc agcatcctgg
     4021 ccctggccgg agcggggctg ggcgggatct ttggccagcg cttggggcca atcgtcacca
     4081 tcctcgccac cagcctggcc ggggcctact tcgtcgttct cgggctgggc gcgtggttcg
     4141 gcctgggtgt aacgactgat gatccgacgg tcacgctcgg ctcagccttc ccgctggtgc
     4201 tttttctgac cgtcgcagcc atcagcggcc tggcccaaca tcaggccttc accttgcggc
     4261 aacgcttcct gcgctaatga acgcaaagag gtcggacgct ggcttgcgcc cgacctcttt
     4321 gtctcgcttc gtggcggagg cgttgccccg gccccgttgc ctgcgctggg gtgggcttct
     4381 gaacccatcc ccgcgcgcgt ctatttctcg accggcggcg tcgccggcgt cgctacgcgc
     4441 ccctgccgcc ggcgcagcca gcggatgagc aggaagatga tcagcagcgg gatggcgacg
     4501 accagcagaa ccggcaggac gaacacgacc agccagatga ccaggttggc cagcgattgc
     4561 agcgcattga tcagcgactc gagcgcttcc ttggccacgc ccctgggctg ccagccggcg
     4621 acctcgatgg gctgggccag ctcgtccggg gtgacgttga cggtgatgga cgagaacgcg
     4681 gccgactgtg acagatattg cattcgcccc ttggtgacgg cgatctcgcc ctccagccgg
     4741 ctgagttcgg cgttaacggc cagggcgtct tcgacccgcg tggcgtcgtc gaggaaggtg
     4801 cgcacccgcg ccgccgtcgc ctccaggttg gtcagctgcg acgacaggtc aacgtactcc
     4861 tcggtcacat cctgccccga cgtggacagg ctctcgacgc tgacggccag cccggcgatg
     4921 gcgtccagca cgctctggaa gccctcggac ggcacgcgaa tctggatgta accggtcttg
     4981 ctcgtctcgc tcgattggta gacgttgctg ctgacgaccc agccgccgcc gttggtggcc
     5041 atcgcggcga tctgggccag ggcggcctcg gtgtcggtgg ccacgatcga catgtcggcg
     5101 ttgcggataa tgagccgctc ctgatcggcg acgggctgcg cgggctgcgc cggctcagcc
     5161 gcgggcgcgt tggccacggt cgaccccgcg gccatgctga aatcgctctc ggcgtcatac
     5221 gcctgcggcg cggcctcttc cacaaccgtt tcgctggccg tctgcgtgac ggcggcgctg
     5281 tcatacgccc taccgccgca ggctacgatc accagcgcca gcactagcaa ccccaccaga
     5341 tatccaactc ggcgtttcat cttcattctc tcctgtctac acggcgctct cattcagttg
     5401 catgtctctg ttgagaggac gctgccgcca ggccaaatgt tccgctacag cccttgttcc
     5461 agccgcttga tcagccggcg cggcaacttt tgctcacgca tgagcacttg tgtcgcggcg
     5521 atgtcgtagg ccacgcggtg aaaggtcatg cgcatggcct cggtgtccag cagggcatag
     5581 gcggcgcggg ggtcgccgtc gcgcggctgg cccacaccgc cggggttgac gatcaggcgg
     5641 tggcggctca ggtcgagcac gtcgccatgc tcgatcaggg cgaagtccac cccaccggtc
     5701 ttttcgtcca gggcaaagac ggccggcacg tgcgtgtggc cgaccaggca gcagggcgtg
     5761 tcgaagtggg cgaagttctc caacgccgtc ggtaggtcga ggatgtactc ccagacaggg
     5821 tggcgcgggc tggcgtgggc cagcgtgaat ggcgcttcga cgcgcagggg cggcagggtg
     5881 ttcaggtagg cgcggttgtc ggccgtcagc ccacggcgcg tccagcgggc cacgtagcga
     5941 gcgtcgctgt tgaaatcctc gtcgtcgagc ttgtccagca cggcccagtc gtggttgccc
     6001 gacagggcca gcggctccaa tgcccgcagc cgttcgacgc attcattcgg ctgcggccca
     6061 tagccgacca gatcgcccag gaaccacaac cgctcccact gtccctcggc ttcggccagc
     6121 actgcctcga acgcgaccag attggcgtga atgtcggaca acaagagaac gcgcatgagg
     6181 gaagtatacc ggaatcgtag gcggtgggca gtgatcggtc gccaacgagc cgggctttgc
     6241 caaagccgtc tagcgaccat gggcctgacc ggtgcgtctg gggtgacttt ggcaaagccc
     6301 tgagttggcc gacagttgtt ttcagcatgt ggccgatttg ggatatactg tccccggata
     6361 gaggctactg gtcagtgggc actatatctt gaccagccat tgatcaccga ctaacgacca
     6421 ctcgtatggc catactcgac gaccggacac tcgatttcgt cagcaccagc cctgcgcaga
     6481 cggagcgcct gggtattcgc ctgggcgagc tgctggagcc gggcgacatt gtctgcctgg
     6541 cgggcgagct gggcgcgggc aagacgacgc tggcgcgggg catcggccgc ggctggggca
     6601 cgcctttgcg cgtcaccagc ccgacgttca tcctggtcaa cgagtacccc cgcccgcgcg
     6661 atggggcgac gctttaccat ctcgactgct accggctgga gcgggccggc gacatcgcca
     6721 ccgtcgggct ggaagacctg ttcgacagcg acgccacggt gatgatcgag tggccggagc
     6781 acattctggc gctgctgccg gccgaccgcc tgtggatcgc ccttagctat gacagcccga
     6841 cccgccgccg cctgcgcctg aacgccggcg ggccgcgggc caccgaccta ctggcggatt
     6901 tccgcaaaaa cgctttcggg gtctaaatca cgatgatcct ggcaattgac acggcaaccg
     6961 actggatcgg cctggctctc cacaacggcg cggccattct ggccgagttc ggctggcgca
     7021 gccggcgcac gcagaccatc gagctggctc cggccgtcgc ccagctatgg ggccgggcgg
     7081 gcgtcgcccc aactgacctg aacgcggtgg ccgtggccat tggccccggc tcctacaccg
     7141 gcctgcgcgt gggcctggcg ctggccaagg ggctggccct ggcccacaac ctgcccctcg
     7201 tcggcgtgcc cacgcttgac atcgtcgccc ggtcggtctg ccggctggag acggacttgg
     7261 tcgtcgtcgc ccaggcgggc cgtacgcgcg tctgggccgg tcagtaccaa tgggtggcca
     7321 agcgtggctg ggagcgcgtt ggcgagccgt tgctgaccaa ttgggaagac ctggccgcgc
     7381 ggctggcgtt gccggtggcc ttcgccggcg agatcgacgc cgccgcggcc aaactcatcc
     7441 gccggaccaa ccgcgccgcg gccatcgccc cgccggccga gtcggtgcgg cgcgcggcga
     7501 tgctggccga aatcggctgg cagcgctgga agcgcaagca gaccgacccg gccggcagcc
     7561 tggcgccgct ctacctgcgc gagccgggct aggcgcgatg ctggccctgc ccccgcccta
     7621 ccgtctgcgg cccatgaccg tggccgacat ccccgccgtc ctggccgttg accgcgcctc
     7681 gttccccacg ccggcgacgg agcagctttt cgtcaacgaa ttgaccgata accagctggc
     7741 ccactaccag gtgttgctgc gcgacgccgc cggcggtgag acgctggtcg gctttgccgg
     7801 cttctggctc atcgccgggg agatccacat cagcaccatc gccgtgtccc cggccgagcg
     7861 cggccgcggc cggggcgagt ggctgctgct gaacttgctg ctgctggcct gcgcgctgga
     7921 gccgctgctc atcactctgg aggtgcgccg cagcaacacc gtcgcccaga ccctctatgc
     7981 caagtaccgc tttgtcgagg tcggcctgcg ccgccgctac taccgcgacc cgcccgaaga
     8041 cgcgctgctc atgaccgtcg atctggccga gcagccggac tactgcgtct ggctggcgga
     8101 acggcggccg agtggccgcg gggaagaata ggccgccgag gcgaggtctg ttaagggtat
     8161 caccggcgag ggctgtataa tcacatgagc ttggcctgcg gcccggcgcg ggcggcggcg
     8221 agcgatccga gagtaaacca cttgagcgga caacacgtct tcatcaccgg cggagccggc
     8281 ttcatcggct gcaatgccgc cgaccacttc ctggggcgcg gcgacgccgt gaccctctac
     8341 gacaatctga cgcggcgcgg cggccgggcc aacctcgact ggctccacgc cacctatggg
     8401 ggcgagcggc tcgccgtcgt ggaggccgac atccgcgact acgaccggct ggcgtcggct
     8461 gtggccgagc ggccgccgga cgtggtcctg cacctggccg ggcaggtagc cgtcacccag
     8521 tcggtggccg acccgcgcga ggacttcgag agcaacgccc tgggcacatt caacgtcctg
     8581 gaggccgtgc gccacggcgc gccgggcgcg gccctgctct atgccagcac caataaggtc
     8641 tacggcggca tggagcacgt cggcatcgtg ccggccggcg agcgctacgc ctatgccgac
     8701 tatccccacg gcatcccgga gacatttccg ctggacttcc actctcccta tggctgctct
     8761 aaaggcgcgg gcgatcagta catgcgcgac tacgcccgca tctatggcct gcggacgctg
     8821 gtgctgcgcc agtcgtgcat ctacggccgg cgtcagtttg gcgtggaaga ccagggctgg
     8881 gtagcccact tcgtcatcgc caccgtcctc gaccggccca ttgccatcta cggcgacggc
     8941 cggcaggtgc gcgatgtgct ctacatcgac gacctgctcg acgcctacca gcgcggcatc
     9001 gaccacatcg acgacctgcg cggcgaggtg ctcaacctgg gcggcgggcc ggacaacaca
     9061 ctgtcgatct ggacggagtt tgggtcgctg ctggccgagc tggccggccg gccggtgccg
     9121 gtgcgctatg gcccgtggcg gccgggtgac cagcgcgtct tcgtggccga catccgccag
     9181 gcgaaggcgc tgctgggctg gcagccgcgc gtctcgccgg ccgaaggcgt gcggcggctg
     9241 tacgagtggg tggcagccaa ccgcgaactg ttctaggcgg ccacaaccca tcgggctaca
     9301 tcgatttcgc ccgtcgtgcg taggggatag caatcctgcg aacggccgga gtgaaatcaa
     9361 tgcgtcattg gctgcgtttg cccctgttcg tcctggttgg cgtgctgacc gcggccgtcg
     9421 gcgagctgca atactcggtc ctcatccgcg gcgactgggc caacctcctc ggctcaatgg
     9481 tcttcaatgc cgtctacctg atcgcggcgt ttgtcgtggt gtgggcgctc ttccggctgc
     9541 tgccgcggcg ggcggccttc ctggcttgcg tgatcctggc ggccgtggcc ggtctgggcg
     9601 ttgagtggtt cctcatcggc aactcccccc ggggcaaccc cgacgccagc caactgggca
     9661 tggccgccta ttgggccggc atggtcgtcg tgccgctcat cgttatggac ggcgacgcgc
     9721 ggctgcgacc gctgaaacga cgtattgccg tctttgccgc ggtctacacg ctggcggttc
     9781 tgctcggcca gtggctccta ccagtgggcg actggcggtt tgctttccac atcttgacag
     9841 tgatcctggg ctatctgggg ttattggtgc tgtgtgtggt gggctatctg cgtaacgccc
     9901 gctcagggag cctagcgttg ctggctgacg aacctggtcg gctggatatt cagcacgatt
     9961 aggatgcttg cagataaggc tgcatggtcg tgctgaattg gggaacggcg cggtgaaatg
    10021 aacggtcttc tgtcagcagc atgacgccca attcaagcgc cacagccaga tattgggcat
    10081 cataggcgct tagacgatac tgctgcgcta actccaggac aagtaagggg cttgcggagc
    10141 gctccttgga tgcaaacaag tcgaccgccg atccccacag ctttttggcc gtctccaggt
    10201 cggcgcctcc ctgacggaca taggtgctaa ggatgttcag gaactcgtct cgccagagat
    10261 caggaacgac ccaatcattg tcaaatcgcc agagggcctg cgcttcgggt gttctatcgc
    10321 cgctgatcaa cagataggca ataatgttgg cgtcaacgac gatcatgccc ggcctgcctg
    10381 ccgtgcctga gacaaccatt catcatcgat atcaaaagcg ccttgcagtg gttgcggcgg
    10441 ctccatatag gtttcgccgc tgaccaggga ttgctccagc aagatcaaaa cttcctgggt
    10501 tacgctgcgc cggttggccc gcgcgcgcgc tttcagtcga ttgtgcaggt cttccgggag
    10561 ttgccggatc agaaggtcag tcatatacgt tgccatcgaa atgatagcac tatgatacaa
    10621 ttgacgcgac tacgcgtcaa gggcttcccc gccgtgtccg cactgcgtac gggttgccca
    10681 ccaaagagtt tcacagtgcc ggctcgccat tgagcgagca tgatataata ctaggcaaga
    10741 caagcgaaga cagggctaca gggtagagca aatggctgaa atgaaaatcg ccgacttgac
    10801 cttagaagag tttcgcgcgc tgattcgcga ggcggtcata gaa
//
LOCUS       DAOSMQ010000040        10670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6194_length_10670_cov_4.847951, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000040 DAOSMQ010000000
VERSION     DAOSMQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6194_length_10670_cov_4.847951"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PK829_02415"
     CDS             complement(<1..437)
                     /locus_tag="PK829_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOU40100.1"
                     /translation="MDDFDYSQFPDLPELFPELSGDLFDQPLTKVKNGVIECPFLPLR
                     DVVLFPQMVMPLFIGRERSLAAIKAANRNHENLIVAAQRDNDAVDPEEDDLFGIGTEA
                     TIGRVLRMPDDTTSVLAQGRRRVKIVAYTQWEPYIRVRARVIE"
     gene            complement(430..1797)
                     /locus_tag="PK829_02420"
     CDS             complement(430..1797)
                     /locus_tag="PK829_02420"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOU40101.1"
                     /translation="MESYQRALDYLYGFINFEHKSLDRYQAAKIDVDRPRRLLALLGD
                     PHRRFPSLHIAGTKGKGSVAAMSAACLRLAGLRVGLYTSPHLREFRERIRILTLDDAD
                     GRISEEAFVETMARVRPAVEQTPGITWFEIVTALAFDTFAAAGVDIAVVEVGLGGRLD
                     ATNVLLPDVAVITSLSLDHTQLLGNTLAEIAFEKGGIIKTGVPVVVAPQQPEALATLE
                     GIAAERGAPLVAVGRDWRYEVTRPGEQQVVIAAGGPAQTFIPPGTAFPVGLRGRHQAD
                     NAAVALAALERARTRFPGLTVADMQRGLATVAWPGRLQTLQSGPGRPTVLVDSAHNDD
                     SAAKLVAALREEYAYDRLVLLFGAPEDKNVAGMLARLLPLADEVIMTTANHPRSATPE
                     QLAALAHDLGHTATITHSVAEGLETALAAAGPGDLVCATGSIIVVGDLLNHWDTLQSD
                     ARSYG"
     gene            1978..2292
                     /gene="rplU"
                     /locus_tag="PK829_02425"
     CDS             1978..2292
                     /gene="rplU"
                     /locus_tag="PK829_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOU40102.1"
                     /translation="MYAIVESGGRQYRAEEGNTFSVEKLPYEVGDQVELTNVLLIANG
                     DAVQVGQPTVAGATVKATVVEQYRGKKILVWKYRAKLRYRRRQGHRQDYTRLRIDEIV
                     AG"
     gene            2325..2600
                     /gene="rpmA"
                     /locus_tag="PK829_02430"
     CDS             2325..2600
                     /gene="rpmA"
                     /locus_tag="PK829_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013189994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOU40103.1"
                     /translation="MAHKKGSGSSRNGRDSNAQRLGIKRYGGEFVGPGVIIVRQHGTK
                     YRPGNNVGVGSDYTIYALIDGQVTFENRFGRKVISVYPVVAAERVGE"
     gene            2606..2794
                     /gene="rpmE"
                     /locus_tag="PK829_02435"
                     /pseudo
     CDS             2606..2794
                     /gene="rpmE"
                     /locus_tag="PK829_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020861530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            2849..3052
                     /locus_tag="PK829_02440"
     CDS             2849..3052
                     /locus_tag="PK829_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOU40104.1"
                     /translation="MPENFDIDMMNLGTRATNALRDAGMTTYGDLLALLKQGDDAVLA
                     LQGIGQTALIKIKRALRDEELIS"
     gene            3149..3736
                     /locus_tag="PK829_02445"
     CDS             3149..3736
                     /locus_tag="PK829_02445"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009792604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU40105.1"
                     /translation="MSKHSHGGIELICGSMFSGKTEEMIRRLRRAVIARQQVQAFKPA
                     IDQRYHIEKVTSHDGLHFEAQPVLRAADILESLDSQTTVVAIDEVQFFDNGIITVCER
                     LADAGIRVICAGLDMDFRGLPFGPMPALMARAEHVDKLHAICVICGEEASRTQRLIEG
                     RPAAFDDPVVLVGAAEVYEARCREHHEVPVAPPEL"
     gene            3739..4305
                     /gene="hpt"
                     /locus_tag="PK829_02450"
     CDS             3739..4305
                     /gene="hpt"
                     /locus_tag="PK829_02450"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOU40106.1"
                     /translation="MRNLHDDIARILIDEPTLAARIKALAAQIDADYAENDDLHLVCV
                     LKGAYMFLADLSRALTRPHSLDFMGISSYGRGITSSGAVQIILDLKKPIDNRHVLIVE
                     DIIDSGRTLGYMRRNLVSRGPASLRICTLLNKPARREVPVEVDYIGFDIPDEFVVGYG
                     LDFDELYRNLPFIAVLKPEMFAHLLNQP"
     gene            4351..5850
                     /locus_tag="PK829_02455"
     CDS             4351..5850
                     /locus_tag="PK829_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU40107.1"
                     /translation="MPRESCHARPMLVFDPLLDRLRPVLAEQPQPVYLVGGIVRDALL
                     ERVSHDIDLIVPRDAISLTFELARRLDLPAYRLDDERDVGRLLVPNSATTVDIARFRG
                     ETLADDLRARDFTINALALPVAARTAAEVIDYHSGRADLAARRLHAIHGHSISDDPVR
                     ALRAARFVVQLGFRPTAETVAAARAAGAMLAGRISPERIRDELTRLLATSAPDRAVAL
                     LDEWGLLAHTLPEIAALADIKQSPPHHEAVLPHTRSVLRYLVAVEERLGDAPSEAAWA
                     AAVAALLQPHRLALSAHLAAPLDGGVSAGQLLRWSALYHDSGKATTQTGDETGRLRFL
                     GHDEVGARLTADRLNKLRFSAEAVRRARDSVAGHMRPLHLATERRPPSRRSAYRYYRA
                     LHEAGVDVVLLSLADHLATYDGAGPAESWAALLAVAGALLDAYFTGYTETVRPARLLN
                     GLEVMALLAIGPGRELGRLLAELEEAQAAGEVVSRDEAEQFLRHHHTGQ"
     gene            complement(5905..6624)
                     /gene="folE"
                     /locus_tag="PK829_02460"
     CDS             complement(5905..6624)
                     /gene="folE"
                     /locus_tag="PK829_02460"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOU40108.1"
                     /translation="MYNANDTLGPNNGNGHRRDFETALLVHPNGHGHTNPLALAQPFV
                     PVAVERKLAIENAVRDILVNVGEDPQREGLQRTPHRVAKMWGEVLEGYEIDPVALVND
                     ALFDVEYDEMVVVKEIEFFSMCEHHMLPFFGRAHVAYIPGAKVIGLSKIPRLVDMFAR
                     RLQVQERMTQQIADMLDDVLAPRGIAVVVEGSHMCGMMRGVEKEHTRMVTTAMRGAFK
                     TDRDLRNEFMEHLRRPSGGLS"
     gene            complement(6841..7218)
                     /locus_tag="PK829_02465"
     CDS             complement(6841..7218)
                     /locus_tag="PK829_02465"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HOU40109.1"
                     /translation="MSTRYFTVEEANALLPELEPLVGRVLALRARASHDSRAMGSLLG
                     DLSSDLGGPLATRLAGEFAEIEDLIARIQAYGCVLKSLEAGLLDFLAERDGRDVLLCW
                     RYGEPAIQYYHEVHSGFQGRRPI"
     gene            complement(7308..7721)
                     /gene="erpA"
                     /locus_tag="PK829_02470"
     CDS             complement(7308..7721)
                     /gene="erpA"
                     /locus_tag="PK829_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOU40110.1"
                     /translation="MDVQHVETVQTETVYLTESAAATVRNLLVQKNVPDYGLRVFVAG
                     GGCSGMQYGMALEAEARDYDHVIEHDGVKIFIDPTSMMYLDQATIDYVDSIMGGGFKI
                     ENPNAVTSCGCGSSFKAKNGGGYEGASGGGCGCGN"
     gene            7960..9741
                     /gene="aspS"
                     /locus_tag="PK829_02475"
     CDS             7960..9741
                     /gene="aspS"
                     /locus_tag="PK829_02475"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOU40111.1"
                     /translation="MLKTHSCGELRREHIGQTVTLAGWVNRRRDMGGVIFIDLRDRAG
                     KTQVVIDAGRSRAAFTAAEGVRSEYVVQVSGQVSARPAGQENSQMPTGDVEVLADRVV
                     VLNPAKTTPFAIDRDDPVDETTRLRYRYLDLRRERMQRNLILRHNAVKFIRDFLGSRG
                     FIEVETPILFKSTPEGARDYLVPSRVHPGKFYALPQSPQQLKQLLMVAGYERYFQIAR
                     CFRDEDLRADRQPEFTQLDMEMSFVERDDILDLIEALMVGMVQAVSLVPLAHTQFPRL
                     SYAEATERFGTDRPDLRYGLELVDIGDIAGRSAFRVFAENVAAGKRVKAICAPGCATY
                     SRKQLDDLESMARAQGAKALAWLAIQPDSGEIRGPIAKFFTVDQLITLTERLEAKPGD
                     LILIASDSKAIVHNVLSTLRTELARRLGYTDQKQLAFAWVIDFPLFEEEMEEGHFAPS
                     HHMFTAPKPEHIPLLDSDPAAVLSQQYDLVCNGYEVAGGSIRIHDQELQAKIMSLIGF
                     SMTQAREQFGHLLEAFEFGAPPHGGIAPGIDRLVALMAGEPNIREVMAFPKTAQATDL
                     MADAPSAVLPRQLDELQLAIKTKDKAG"
     gene            complement(9738..10016)
                     /locus_tag="PK829_02480"
     CDS             complement(9738..10016)
                     /locus_tag="PK829_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40112.1"
                     /translation="MSEYIEIDAEVGDDGLIVFETNLPLTAAGQEERYDSPAALDEGS
                     PVAQALSVVPGIAGLTISGGRMILAAAVDADWHAIIADVTAALKEFFL"
     gene            complement(10024..10662)
                     /locus_tag="PK829_02485"
     CDS             complement(10024..10662)
                     /locus_tag="PK829_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HOU40113.1"
                     /translation="MKNTSIHNTLVDMLGKPIAECNALCIATALYAIPNGAVLAWEFI
                     SGHEPRCPMCELGWKSLGVLELTALPSIKEEYWRPMVQETDVLLVSGGDPVYLSYWMR
                     QSGLADLLPSLRAVYVGMSAGSMVMAPNIGREFVSWTQSPEGDKGLGMVDLAIFPHLD
                     HEELPHNTMANAERWAAGIRTPGYAIDDETALKVIDGAIEVVSEGHWKRFAP"
BASE COUNT         1911 a         3562 c         3344 g         1853 t
ORIGIN      
        1 tcttcgatga cacgcgcgcg gacgcggatg tagggttccc attgggtgta ggcgacgatc
       61 ttgacgcggc gccggccctg agccaggacg ctggtagtgt cgtcgggcat gcgcaggacg
      121 cggccgatgg tcgcttcggt gccgatgccg aaaaggtcgt cctcctccgg gtcgacagcg
      181 tcgttgtcgc gctgggcggc gacgatgagg ttctcgtggt tgcggttggc cgccttgatg
      241 gccgccagcg agcgctcgcg gccgatgaac agcggcatga ccatctgcgg aaacaggacc
      301 acgtcgcgca agggcaggaa cgggcactca atgacaccgt tcttgacctt ggttaagggc
      361 tggtcaaaca ggtcgccgct cagttcgggg aacaactccg gcaagtcggg gaactgagag
      421 taatcgaaat catccatagc tacgtgcgtc cgattgtaaa gtgtcccaat gattaagcaa
      481 atcgcccacg acgatgatcg atccggtggc acagaccaga tcgcccggcc cggccgcggc
      541 cagggccgtc tccagcccct cggcaacgct gtgggtgatg gttgccgtgt gtcccaggtc
      601 atgggccaga gcggccagtt gctccggcgt ggccgagcgg gggtgattgg ccgtggtcat
      661 aatgacctcg tcggccagcg gcaacagccg cgccagcatc ccggccacgt tcttgtcctc
      721 cggcgcgccg aacagcagca ccaggcggtc gtaggcgtac tcctcgcgca gcgcggccac
      781 cagcttggcc gccgagtcgt cgttgtgggc gctatcgacc aggacggtcg gccggcccgg
      841 ccctgactgc aaggtctgta agcggccggg ccaggccacg gtcgccaatc cgcgctgcat
      901 gtcggccacg gtcaggccgg ggaagcgcgt ccgcgcccgc tccagcgcgg ccagggcgac
      961 ggcggcattg tcggcctggt ggcggccgcg caggccaacg gggaacgctg tgcccggcgg
     1021 gatgaacgtt tgcgccgggc cgccggcggc gatgacgacc tgttgctcgc ccggccgcgt
     1081 cacctcgtag cgccagtcgc ggccgacggc gaccagcggc gcgccgcgct cggcggcaat
     1141 gccctccagc gtggccagcg cctccggctg ttgcggcgcg acgaccaccg gcacgcctgt
     1201 cttgatgatg ccgcccttct cgaaggcgat ctcggccagc gtgttgccca gtagctgggt
     1261 gtggtcgagg ctcaggctgg tgatcacggc tacgtccggc agcagcacgt tggtggcgtc
     1321 cagccgcccg cccaggccca cctcgacgac ggcgatgtcc acgccggcgg cggcgaaggt
     1381 gtcgaacgcc agcgcggtga cgatctcgaa ccaggtgata cccggcgtct gctccacggc
     1441 cgggcgcacg cgcgccattg tctcgacgaa ggcctcttcg gagatgcgac cgtcggcgtc
     1501 gtcgagcgtc aggatgcgga tgcgctcgcg gaactcgcgc aggtggggcg aggtgtagag
     1561 gccgacgcgc agcccggcca ggcgcaggca ggcggctgac atggccgcca ccgagccttt
     1621 gcccttggtg ccggcgatgt ggagggacgg gaagcgccgg tgcgggtcgc ccagcagggc
     1681 cagcagccgc cgcggccggt cgacgtcgat cttggccgcc tggtagcgat cgagggactt
     1741 gtgctcgaag ttgatgaatc cgtaaagata atcgagggcg cgctgatacg actccatagg
     1801 ccggattgta gcgtatccgc tcggcgctgt ccggcgtttg acaacgcgcc ggccatcgcc
     1861 tataattctt cttcgcgacg ccggtcacca accacgccgg cagcgcgcag tgaacaagca
     1921 accaagttga ccagggccgg catcgtgtac ggatgccggt tctttttgga ggttatggtg
     1981 tacgcaattg tggaaagtgg tggccggcaa taccgcgccg aagagggcaa taccttttcc
     2041 gtcgaaaaac tgccctatga agtcggtgac caggtggagt tgactaacgt cctcctcatc
     2101 gccaatggcg atgccgtgca ggtgggacag ccgaccgtcg ccggggccac cgtgaaagcg
     2161 actgtcgtgg agcaataccg cggcaagaag atcctcgtct ggaagtaccg ggcgaaactg
     2221 cgctatcgcc ggcgccaggg tcatcggcaa gattacaccc gcctgcgcat cgacgagatc
     2281 gtcgcgggct agtccggcca gatagaggcc aggagagttg taacatggca cacaagaaag
     2341 gaagcgggtc ttcccgcaac ggccgcgaca gcaacgccca acgattgggc atcaagcgct
     2401 atggcggcga attcgtcggc cccggcgtga tcatcgtccg ccagcatggc acgaagtatc
     2461 gtccgggcaa caacgtcggc gttggcagcg actacacgat ctatgccctc atcgatggcc
     2521 aggtgacctt cgagaatcgc tttggccgca aggtcattag cgtctatcct gtggtcgcgg
     2581 ccgagagggt cggggagtaa gcaacatgaa ggacaacacc catcccaagt ggtaccccga
     2641 cgctaaggtg atcgtcgaag gcgaggtcgt catgaccgtc ggttcgaccc ggccgcagat
     2701 cgtggttgag gtctggtcgg gcacgcaccc gttctacact ggcacgcagc gcctgatgga
     2761 caccgagggc caggtcgatc gcttcatgcg tcgcctccag cgccgccagg acatccagac
     2821 gacgaccgag accaccaagc aacagcgcat gccagagaac ttcgacatcg acatgatgaa
     2881 tctgggcacg cgggctacca acgccctgcg tgacgccggc atgaccacct atggcgacct
     2941 gctggcgctg ctgaagcaag gcgacgacgc cgtgctcgct ctgcagggca tcggccagac
     3001 ggcgctgatc aagatcaagc gcgcgctgcg cgacgaggaa ctgatcagct aagcgggcag
     3061 ccgccctctt tgcctacgtc ccgtttcggg cgataatggg caggtacgtc aaaagacgtg
     3121 tctgcccttt ttattttcct ggaggcttat gagcaagcat tcgcacggag ggatcgaact
     3181 catctgtggc agcatgttta gcggcaagac agaggagatg atccgtcgcc tgcgtcgcgc
     3241 cgtcatcgcc cgacagcagg tgcaggcctt caagccggct atcgaccagc gctaccacat
     3301 tgagaaagtg acttcccacg atggactgca cttcgaggcc cagcccgtcc tgcgcgccgc
     3361 cgacatcctg gagtccctcg actcgcaaac gacggtcgtg gccatcgacg aggtgcagtt
     3421 cttcgataac ggcatcatca ctgtttgtga acggctggcc gacgccggca ttcgcgtcat
     3481 ctgcgccggg ctagacatgg attttcgcgg tctacccttt ggccccatgc ccgcgctgat
     3541 ggcccgcgcc gagcacgtcg acaagctgca cgccatctgc gttatctgtg gcgaagaagc
     3601 cagccggacg cagcgtctca ttgagggccg gccggcggcg ttcgacgatc ccgtcgtcct
     3661 ggtcggcgcg gccgaggtct acgaggcgcg ctgtcgcgag caccatgagg tgcccgtcgc
     3721 cccaccggag ctatagctat gcgcaatctt cacgacgaca tcgcccgcat cctcattgac
     3781 gagccaacgc tcgccgcgcg catcaaggcg ctggcggcgc agatcgacgc cgactatgcc
     3841 gagaacgacg atctgcacct ggtttgcgtg ctcaaagggg cctacatgtt tctggccgac
     3901 ctgagccggg cgctgacgcg cccccacagc ctcgatttca tgggcatctc cagctatggc
     3961 cgcggcatca ccagcagcgg cgcggtgcag atcatcctcg acctcaagaa gcccatcgac
     4021 aaccgccatg ttctcattgt cgaggacatc atcgacagtg gccgcaccct cggttatatg
     4081 cgccgcaacc tcgtttcccg cggcccggcg tcgctacgca tctgcacctt gctcaacaag
     4141 cccgcccggc gcgaagtgcc ggtcgaggtc gattacatcg gtttcgacat ccccgacgag
     4201 ttcgtcgtcg gctatgggct ggacttcgat gaactgtatc gcaatctgcc cttcatcgcc
     4261 gtgctaaagc cggagatgtt cgcccacctg ctcaatcagc cgtagggcgc tttgggaaaa
     4321 tcgcgccacg cgaatcacgc cacgcgaatc gtgccacgcg aatcgtgcca cgccagacca
     4381 atgctcgtct tcgacccatt gctcgaccgc ctccggccag tgctggccga gcaaccccaa
     4441 cccgtctacc tggtcggtgg gatcgtgcgc gacgcgctgc tagagcgggt cagccatgat
     4501 atagacctga tcgtgccgcg cgacgccatt tccctgacct tcgagctggc ccggcggtta
     4561 gacctgcccg cctaccggct ggatgacgag cgcgatgttg gccgcctgct cgttcccaat
     4621 agcgccacca cggtcgatat cgcccgcttt cggggcgaga cgctggccga cgacctgcgg
     4681 gcgcgcgact tcaccatcaa cgccctggcc ctgccggtgg cggcgcggac cgcggccgag
     4741 gtcatcgact accacagcgg ccgggccgat ctggctgcgc gccgcctgca cgccatccac
     4801 ggccactcca tcagcgacga cccggtgcgc gccctgcgag cggcccgatt cgttgtccag
     4861 cttggctttc ggcccacggc cgagacggtc gccgccgctc gcgccgccgg agccatgctg
     4921 gccgggcgca tctcgccgga gcgcatccgt gacgaactga cccggctgct ggccacgtcc
     4981 gcgcccgatc gcgccgtcgc cctgctggac gagtggggtt tattggccca cacgctgccg
     5041 gagatcgccg cgttggccga catcaagcag tccccgcccc accacgaggc cgtgctgccc
     5101 cacacgcgca gcgtcctgcg ctatctggtt gctgtcgaag agcgcctggg cgacgcgccg
     5161 tccgaagcgg cctgggctgc ggccgtggcc gcgctgttgc agccccatcg tctcgccctg
     5221 tcagcccacc tggcagcgcc tcttgacggc ggcgttagcg ccggccagct tctgcgctgg
     5281 tcggccctct accacgacag cggcaaggcg acgacgcaga cgggcgacga aaccggccgc
     5341 ctgcgcttcc tgggccacga cgaggttggc gcgcgcctga cggctgatcg gctgaacaag
     5401 ctccgtttca gcgccgaagc cgtgcggcgc gcccgcgaca gcgtcgccgg acacatgcgc
     5461 ccgctgcatc tggccacgga gcgccggccg ccgtcgcgcc ggtcggccta ccgctactac
     5521 cgcgccctgc acgaagccgg ggtcgatgtc gtcctgttgt cgctggccga ccacctggcc
     5581 acctacgacg gcgcaggccc ggcggagagt tgggcggcgc tgttggcggt ggctggggcg
     5641 ctgctggatg cctacttcac gggctacaca gagaccgtgc ggccggcgcg gctgctgaac
     5701 gggctagagg taatggcgtt gctggccatc gggccagggc gggagttggg ccggctgctg
     5761 gccgagctgg aggaagcgca ggctgccggc gaagtggtta gccgcgacga ggccgagcag
     5821 ttcctgcgtc accaccacac tggccagtaa accaaccgcc cgcaacgatg cgcgggcggt
     5881 tggcttgaga cccacagaga agagttaact caggccgccg ctcggccggc gcaggtgctc
     5941 catgaactca ttacgcagat cgcggtcggt cttgaacgcg ccgcgcatgg ccgtggtcac
     6001 catgcgggta tgctccttct cgacaccccg catcatgccg cacatgtgcg agccttcaac
     6061 cacaacggcg atgccgcgcg gagccagcac atcgtcgagc atgtcggcga tctgctgggt
     6121 catgcgctcc tgaacttgca ggcggcgggc gaacatatcg acaaggcgcg ggattttcga
     6181 caggccgatc acctttgcac cggggatgta ggccacgtgg gcacggccga agaagggcag
     6241 catgtgatgc tcgcacatgc tgaagaattc gatctccttg accacgacca tttcgtcgta
     6301 ttccacatca aacagcgcgt cgttgaccag ggccacgggg tcaatttcgt acccctccag
     6361 aacttcgccc cacattttgg caacgcgatg gggtgtccgc tgcaggccct cgcgctgtgg
     6421 gtcttcgccg acgttcacca gaatgtcgcg cacggcgttc tcaatggcca gcttgcgctc
     6481 gaccgccaca ggcacaaatg gctgggccag cgccaacggg ttggtgtggc cgtggccatt
     6541 ggggtgcacc agaagcgctg tctcaaagtc gcgccgatgt ccattgccat tgttcgggcc
     6601 gagcgtatcg ttcgcgttgt acattgttga ctccttcatg tttcgcacag cgccagcctt
     6661 ctggcccgtg ttcgttcttt ttgtcttacc tggctgtcca cctttgctgg acaacacgat
     6721 gagtagtata gcatttgtcc atgttttgtc aatagtttcg tggcattttg cccaccaaaa
     6781 acgaccacag gcgatcggtc gatcgcctgt ggtcgtaacg tcaggggtgg gcgcggcgcg
     6841 ctagatgggc cgtcgcccct gaaagccact gtgcacttcg tggtagtact gaatagccgg
     6901 ttcgccatag cgccagcaga ggagcacgtc ccgaccgtcg cgctcggcca ggaagtcgag
     6961 caagcccgcc tccaggctct taagcacgca gccgtaggcc tgaatgcggg caatgaggtc
     7021 ttcgatctcg gcgaactcgc ccgccaggcg cgtcgccagc ggcccgccga ggtcgctgct
     7081 caggtcgccc agcagcgacc ccatggcccg gctgtcatgg ctggcccgcg cccgcagggc
     7141 caagacgcgg ccgaccaacg gctctaactc cggcaaaagt gcgttggctt cttcgacagt
     7201 aaagtagcgt gtgctcatga ccggcctccc ttcacaaccg atggaaaaca aaaagggtgt
     7261 cggccacagt ggcgacaccc tcaacaaatc acaaatctgc ctggctctca gttgccgcag
     7321 ccgcaaccac cgccggacgc gccctcgtag ccgccgccat tcttggcctt gaacgacgag
     7381 ccacagccgc aggaagtcac cgcgttgggg ttctcgattt tgaagccgcc gcccatgatg
     7441 ctatcgacgt agtcaatggt cgcctggtcg agatacatca tgctggtcgg gtcgatgaaa
     7501 atcttcacgc cgtcgtgctc aataacgtga tcatagtcgc gggcctcggc ctccagggcc
     7561 atgccatact gcatgccgga gcagccgccg ccggccacaa agacgcgcag gccatagtcg
     7621 ggcacgtttt tctggaccag caggttgcga accgtcgccg cggccgattc ggtcagataa
     7681 acggtttctg tctggacagt ctctacgtgt tgtacatcca tgtctcttca agtcctcgcc
     7741 acaatcggcc ttgtcatgcc gttgatgata cgggcggatt ctagcaatga tggcgcggaa
     7801 agtcaatttt tatgaaccaa ttctactaaa tgccgcgtcg cggccggcgt tgtgtccgcg
     7861 cgcgggctat gctagaatcg gcccttgcct cgcgccggat cgtggatcga ggggcgacat
     7921 catcacagcc ttggcgctca tttttgagga aggatagcaa tgctgaagac gcacagctgc
     7981 ggcgagcttc gccgcgaaca catcggccag accgtcaccc tggcgggctg ggtcaatcgc
     8041 cggcgcgaca tgggcggcgt gatcttcatc gacctgcgcg accgcgcggg caagacgcag
     8101 gtggtcatcg acgccggccg ctcgcgcgcg gccttcaccg cggccgaggg cgtgcgcagc
     8161 gagtacgtcg tgcaggtcag cggccaggtc agcgctcgcc ccgccggcca ggagaacagc
     8221 cagatgccga ccggcgacgt cgaagtgctg gccgaccgcg tcgtcgtcct gaacccggcc
     8281 aagaccacgc cgtttgccat cgaccgcgac gaccccgtcg acgaaaccac tcgcctgcgc
     8341 taccgctacc tcgacctgcg ccgcgagcga atgcagcgca acctcatcct gcgccacaac
     8401 gcggtcaagt tcatccgcga tttcctgggc agccgcggct tcatcgaagt ggagacgccc
     8461 atcctcttta agagcacgcc ggagggcgcg cgcgactatc ttgtgcccag tcgcgtccac
     8521 cccggcaagt tctacgccct gccccaaagc ccgcagcaac tcaaacagtt gctgatggtc
     8581 gccggctacg agcgctactt ccagatcgcc cgctgcttcc gcgacgaaga cctgcgcgcc
     8641 gaccgccagc cggaattcac ccagctagac atggaaatga gcttcgtcga gcgcgacgac
     8701 attctcgacc tgatcgaggc gctcatggtc ggcatggtgc aggcggtcag cctcgtgccc
     8761 ctggcccaca cccagtttcc ccgcctcagc tacgccgagg ccaccgagcg cttcggcacc
     8821 gaccggcccg acctgcgcta tggtctggag cttgtggaca tcggcgacat tgccggccgc
     8881 agcgccttcc gcgtcttcgc cgagaacgtc gccgcgggca agcgcgtcaa ggccatctgc
     8941 gcgcccggct gcgccaccta tagccgcaag cagctcgacg acctggaaag catggcccgc
     9001 gcccaggggg ccaaggcgct ggcctggctg gcgatccagc ccgacagcgg cgagattcgc
     9061 ggccccattg ccaaattctt caccgtcgat caactcatca cccttaccga gcgcctggag
     9121 gccaaaccgg gcgacctgat cctcattgcc agcgacagca aagccatcgt tcacaatgtg
     9181 ctcagcacgc tgcgcacgga gttggcccgc cggctaggct acaccgacca gaagcaactg
     9241 gccttcgcct gggtcatcga cttccctttg tttgaggaag agatggaaga gggccacttc
     9301 gcccccagcc accacatgtt caccgcgccc aagccggagc acattcccct gctcgatagc
     9361 gacccggcgg ccgtgctcag ccagcaatat gacctggtct gcaacggcta cgaggtcgcc
     9421 ggcggcagca tccgaattca cgaccaggag ctgcaagcca agatcatgtc gctgatcggc
     9481 ttctcgatga cacaggcgcg ggaacagttc ggccacttgc tggaggcgtt cgagttcggc
     9541 gcgccgcccc atggcggcat cgcccccggc atcgaccggc tggtggccct gatggccgga
     9601 gagccgaaca ttcgcgaagt gatggccttc cccaagacag cccaggcgac tgacctgatg
     9661 gccgacgcgc cctcggcagt cctgccgcga cagctggacg agctgcaact ggccatcaag
     9721 accaaagaca aggccggcta gagaaagaac tccttcagcg cggcggttac gtcggcgatg
     9781 atggcgtgcc aatcggcatc aacagcggcg gccaggatca tccggccgcc gctgatcgtc
     9841 aggccagcga tgccgggcac gaccgacagc gcctgggcca cgggcgagcc ttcgtccagc
     9901 gccgccggcg aatcataacg ctcttcctgg cccgcggctg tcagtggcag attggtctcg
     9961 aagacaatca gcccgtcgtc gcccacttcg gcatcgatct cgatgtactc ggacatatcc
    10021 ctattagggc gcaaaccgtt tccagtggcc ttcggagaca acttcaatgg ctccgtcgat
    10081 cactttgagg gcggtctcgt cgtcaatcgc gtaccccggt gtccgtatgc cggccgccca
    10141 tcgttcggca ttggccatcg tgttgtgcgg caactcctca tgatccaggt gcggaaagat
    10201 cgccagatcg accattccca gtcccttgtc gccctcaggg gactgagtcc agctgacgaa
    10261 ctcccggcct atgttaggag ccatcaccat gctcccggcg ctcattccca cgtagaccgc
    10321 gcgcagcgac ggcaagaggt ctgccaaccc ggactgccgc atccagtagc tcagatatac
    10381 ggggtcgcca ccactcacta gcaaaacgtc ggtctcctgg accatgggcc gccaatattc
    10441 ttctttgatg ctggggagcg ccgtgagttc cagcactccc agggacttcc aacccaattc
    10501 gcacatgggg cacctgggtt cgtggccgct gatgaactcc cacgccagga cggcaccgtt
    10561 gggtatggcg tacaacgcgg tggcgatgca aagagcgttg cactcggcaa tgggcttgcc
    10621 cagcatgtca accaatgtgt tgtgaatact ggtgttctta atgccggcgg
//
LOCUS       DAOSMQ010000041        10669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6196_length_10669_cov_5.068212, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000041 DAOSMQ010000000
VERSION     DAOSMQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10669
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6196_length_10669_cov_5.068212"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PK829_02490"
     CDS             <1..687
                     /locus_tag="PK829_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU40114.1"
                     /translation="HEGTPIDAVGGPIETIGETDEAQAIALAMSSWFGVGGSAFRTID
                     DREMLVETVAFPAYRRRTLQQLGPFDEELVRNQDDEYNYRLLKHGGRILLSPDIRSRY
                     YSRGTMRSLWRQYYQYGYWKVRVMQKHPRQMRARQFVPPAFVVGLFGSAALGLVWRPF
                     RALLAVVVGLYLAANVVASLSVSRAHAPRLLLIHPILHLSYGLGFLAGLARFRHRFAG
                     PAGRPAPQSR"
     gene            773..2698
                     /locus_tag="PK829_02495"
     CDS             773..2698
                     /locus_tag="PK829_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOU40115.1"
                     /translation="MDDPQVNDILNFSRRQFLHGAGALTAGLVLGGAPKLGRLGLALP
                     RAQAGAAVDVTAAPFGAKGDGVSNDRAAFQAAIAAAIQQRKPLWVPQPASFYRLELDN
                     ANPQLDVPGDLAIVGAGRATTLLRFTVAGPQAGATYSGFYVHNGVRFQAADLRLEEDA
                     APDGYALHGFNFQSGPADHQCLIEAVDADGFTHVAMAGSSGSGDSRGELFLAIRDCDF
                     SPRTECCVAFWTAETGHKRLHVYDSYFHDNVSNHLVYCHPHNSVHIENTRFDGTPNWS
                     FHLQGSAISGEPEYQRFIGCWFGPNVGRGIITHGGPTTHPRPEIVNCLFECSNAIQIR
                     SDVLIDGCYFTNNRDAQSNSAFVASIENEPWEVTMRNCIFAPKRDVLPYVDLRLADIT
                     ATIANCQFYHQGSGSILALGKSASNVSTVSNCLFYVRPDNGSQAISIEVEDGQTTIAN
                     CRFHGRAPSDRAVIHCQRSSGTLSDGAALRIDDCTFQSVTGGGLFYVDDGPGNSWRGR
                     ISGGNNRINDWQSASPMVVVNAPQAPFYARLLPVAGPAPLGLVAAATTVVSSNYDAYE
                     ISTPGEISALHWWSADGIADPLFSGTIRLTAVTPFALLASGNIRLAGGAARRDVAAGT
                     TVRLAYDSEQGVWSEVA"
     gene            2740..3423
                     /locus_tag="PK829_02500"
     CDS             2740..3423
                     /locus_tag="PK829_02500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU40116.1"
                     /translation="MSERQRVHEIYARRQGHSERYSLGNPAHLFIMQGRQRAVLRRLR
                     REGLWPLAGHDILEVGCGSGGVLLELLTYGADAGRLHGTDLLGERVAVARRRLPHVAV
                     TCADGRSLPYPDQSFDLVLQFTMFSSILDQGLCYTVANEMRRVLRPGGLLLWYDFWLN
                     PTNRQTRGIRPREIRQYFPDSQYVFERITLAPPLTNHLVPFSWVAALSLERLGIFNTH
                     YLAAIRPNR"
     gene            3427..5100
                     /locus_tag="PK829_02505"
     CDS             3427..5100
                     /locus_tag="PK829_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU40117.1"
                     /translation="MNESIASQPVIDAKLRSGLTDLRHTVDNIARRTLDVTIAALGVL
                     VLLPGLVLLAVLIKRDSPGPVFYWGERAGRHGRPFRILKFRTMYETGDSYNGPRVTGQ
                     GDPRITPFGQWLRETKVNELPQLWNVLKGDMSLVGPRPEDRDFVGHWPADVREVLLSV
                     RPGITSPASVLYRDEESMLSSGNVVDQYLRIILPSKLRLDLIYVHHRSFLTDLDVLFW
                     TLMTLLPRLKTRQVPEHLLFWGPLARFISRYMSWFVLDSVVAFVAVAATGIIWRSNGP
                     LELGPSLALGVALAIALIFSLVNGVLGVGRIVWSRADSEDAITLAISSGLTTAILLAT
                     NWLWQPRAIPVPYLGHRLLPSGLIVVSGLLAFAGFLLLRYRMRVITGLSQYWMALRGG
                     TLAVGERVLIIGAGEAGNMAAYLLRKLEAGQAFSIIGLVDDAPRKLGARVGGLRVLGP
                     TVDVPQLVRVHDIGLILFAIENIRPAERERILQICRATGAQTVVLPDVLASLRTQLAN
                     SAPAAPAAPMPSGAEMDSWLRQLDGLIAAEAWDAVREQVRTRQRQIVSD"
     gene            5201..6193
                     /locus_tag="PK829_02510"
     CDS             5201..6193
                     /locus_tag="PK829_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU40118.1"
                     /translation="MSDWTGKRVLVTGAGGFIGSHLTESLARAGAGVRAFVRYNSRGD
                     AGLLRQLPAEALAEVEIVAGDLRDGAAVERAVAGCDVVFHLGAIISIPYSYRHPLETA
                     ETNFMGTLAVLLACRAHGVERLVHTSTSEVYGTAQFTPMDESHPLQGQSPYSASKIGA
                     DKLVESFYRSYDLPTVTVRPFNTYGPRQSARAVIPTIITQALTADCIRLGSLTTRRDF
                     TYVGDTVAGFLRAALAPDVAGEEINLGTGDDIAIGELAERVIALVGRPVAIVTEAERL
                     RPEKSEVRRLLSGNDRARRVLGWQPAWSLDDGLRQTVAWIGDHLDFYRVGRYEV"
     gene            6203..6970
                     /locus_tag="PK829_02515"
     CDS             6203..6970
                     /locus_tag="PK829_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU40119.1"
                     /translation="MKAVILAGGKGTRLKPYTTILPKPLMPIGDMPILEILLRQMRRA
                     GVDEAVLTVGHLAEMLRLFFQDGRRLGMDIRYSYEDAPLGTAGPLSLVEGLDDTFLVS
                     NGDVLTTLPLAELLAHHHDSGAAATIAMHRRQVYIDLGVIQQNEAGHVTGYIEKPTYD
                     FMVSMGIYVFEPRVLDFIPRNAYLDFPDLVKRLIAAGEIVSGFPFDGYWQDLGRADDY
                     ERAVEDFDRLRDAFLPPELPVAASANGAISARNGRGG"
     gene            6975..8180
                     /locus_tag="PK829_02520"
     CDS             6975..8180
                     /locus_tag="PK829_02520"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU40120.1"
                     /translation="MAETNGQWRVPLADIDLGPEEEAAVLAVLRRRWLSMGSETAAFE
                     ADFAALTGAAHALAVTNCTAALHLAGLALGWGPGDEVIVPSLTFVATANAVRYTGATP
                     IFADIRSEDDLSLSPDDVAARITPRTRAIVAVHYGGYAADMPALLALAERHGLDVVED
                     VAHAPGGSLDDRALGTWGRVGCFSFFANKNMTTGEGGMLTTGDDALAERLRLLRSHGM
                     TTLTWDRHRGHAFSYDVVAPGYNYRIDELRAAIGRAQLAKLPANNGRRAAIDAAYRAQ
                     LPVAAPELGLPYARAGGVSAHHLRPALLPPGIDRTRFMTEMKARGIQTSIHYPPIHLF
                     SLYRRDGGPSLPLTEAVAAREVTLPLYPGMSSGDVELVVAATAEALAAARVDATASAS
                     EVAAGGRAN"
     gene            8280..10217
                     /locus_tag="PK829_02525"
     CDS             8280..10217
                     /locus_tag="PK829_02525"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOU40121.1"
                     /translation="MELKLILQLVRRWAWLLTIGVLVGGGIGYVLSIYQTPEYEARAK
                     ILVMEPVRNNSASTSTTLKANKEMADTYLELLFTQPILDATSQKVGATVHTSQIHADR
                     VGETGLLEVVIRDTDPERAALTANTLVQVLIDQNEALQSSRFSSSEGSLQAQVAEVEQ
                     ELSNLSDQVEKRSEETVAAQTEQIETQKVALEQQINALLGEITRLEQDIDDLTPKVIV
                     AGQPPAALTLEQRNLLTAKRTELAQKQFQLELAQNNYAELLTPLPSTGSAEDSTNLRD
                     IEQANLELYRQLYSTLLSNYEAVRLARLENTPNVVQVERAAPSNVPVQSGPLRYIIMG
                     AIMGLLLTGTLAFTLEYLDDTLKTPQDVANVLGLPVVGYVVNEAGIEKGEGTPYVSSN
                     PRSPLAETFRTLRTNLEFASVDKPLKTILITSPGSSEGKTTVASNLAAVMAQANKRVI
                     LLEGDLRRPRVHRALGMSNQIGLSDVFRGQMDIRDVARYSKVKDLAVITSGSLPPNPA
                     ELLGSARMSQILARLVESASVVIIDSPPFLVTDATVLSAKVDGVLLVIQPGKTHAEAA
                     KAMLAQLNRAGAHVVGVVLNRVPRKNANYYGYYRYENDYTIESTPAEAPAVNGDGRRA
                     GKGLATMFSGKPGKIGEEVVS"
     gene            10268..>10669
                     /locus_tag="PK829_02530"
     CDS             10268..>10669
                     /locus_tag="PK829_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40122.1"
                     /translation="MPQSATYRLPLLRRPGAAGGVNRTPGQCPFLGTPADPGTALAFP
                     SEANHCFRSRIPIPISSIHQENYCLSHQYETCPVYRQYATTEERAEAASVPVAAVALA
                     APEPHWAGESVGSAIAPATTAAGMALGAGRPS"
BASE COUNT         1742 a         3657 c         3475 g         1795 t
ORIGIN      
        1 catgagggca cgcccatcga cgccgtcggc ggccccatcg aaaccatcgg cgagacggac
       61 gaggcccagg ccattgccct ggccatgagt tcctggttcg gcgtcggcgg ctcggccttc
      121 cgcaccatcg acgaccggga gatgctggtg gagacagtgg ccttccccgc ctatcgccgc
      181 cgcacgctgc aacagctcgg ccccttcgat gaggagcttg tccgcaatca ggacgacgag
      241 tacaactacc gcctgctgaa gcacggcggg cgcatcttgc tctcgcccga cattcgctcg
      301 cgctactaca gccgcggcac gatgcgctcg ctctggcggc aatactacca gtacggttac
      361 tggaaggtgc gcgtgatgca gaagcacccg cgacagatgc gggcgcggca gtttgtgccg
      421 ccggcctttg tcgtcgggct atttggctcg gcggcgctgg ggctggtgtg gcggccgttc
      481 cgcgccctgt tggccgtggt ggtgggcctc tatctggcgg ccaacgtggt cgcctcgctg
      541 tccgtcagcc gcgcccacgc gccgcggctg ctgctcatcc atccgattct gcatctgagc
      601 tacgggctgg gctttctggc gggcctggcc cgatttcgcc atcggtttgc cggccccgcc
      661 ggccgccccg cgccgcagag ccgatagcgt cgggcagcga cgcgcagtct ttcgaaagtg
      721 gccgcggacg gttggcccat ccattacccc cgacgtccgc aagcaggtga acatggacga
      781 cccacaggtc aatgacattc tgaacttctc gcggcggcag tttctccacg gcgcgggcgc
      841 gctgacagcg gggcttgtcc tgggcggggc gccgaagctg ggccgactgg gcctggcgct
      901 cccgcgcgcc caggcggggg cggccgttga cgtgaccgcc gcgccgtttg gggccaaggg
      961 cgacggcgtc agcaacgacc gcgccgcctt tcaggcagcc attgccgcgg ccatccagca
     1021 gcgcaaaccg ctctgggtgc cgcagccggc gtcgttctat cgtctcgagt tggacaacgc
     1081 caatccccag ttggacgtgc ccggcgatct ggccatcgtc ggcgcgggtc gggcaacgac
     1141 cttgctgcgt ttcaccgtcg ccgggccaca ggcgggcgcg acttattcgg gcttctatgt
     1201 ccacaacggc gtgcgcttcc aagccgccga cctgcgactg gaagaagacg ccgcgcccga
     1261 cggctacgcg ctccacggct tcaacttcca gagcggcccg gccgaccacc agtgcctgat
     1321 cgaagcggtg gatgccgacg gcttcaccca cgtggccatg gccggctcct ctggctctgg
     1381 tgacagccgc ggcgagctgt tcctggccat ccgcgactgt gatttcagcc cgcggacgga
     1441 gtgctgtgtt gccttctgga cggccgagac cggccacaag cgcttgcacg tctacgacag
     1501 ctacttccac gacaacgtca gcaaccacct ggtctattgc cacccccata actcggttca
     1561 catcgagaac acgcgctttg acggcacgcc caattggtcg ttccacctgc aaggctcggc
     1621 catctccggg gaaccggagt accagcgctt catcggctgc tggtttgggc caaacgtcgg
     1681 ccggggcatt atcacccacg gcgggccgac gacgcacccc cgtccggaga ttgtcaactg
     1741 tctgttcgag tgcagcaacg ccatccagat ccgctccgac gtactgatcg acggctgcta
     1801 cttcaccaac aaccgcgacg cccagagcaa cagcgctttt gtggcctcca ttgagaatga
     1861 gccgtgggaa gtgacgatgc gcaactgcat cttcgcccct aagcgcgacg tgttgcccta
     1921 cgtggacctg cgtctggctg acatcacggc caccatcgcc aattgccagt tctaccatca
     1981 aggctccggc tcgatcctgg ccctgggcaa gagcgcgagc aacgtctcga cagtcagcaa
     2041 ctgcctcttt tacgtccgcc ctgacaacgg ttcgcaggcg atcagcatcg aggttgagga
     2101 cggccagacg acgatcgcca actgccgttt ccacggccgc gcccccagcg accgggccgt
     2161 catccattgc cagcgcagca gcggcacgct gagcgacggg gccgcgctgc gcatcgacga
     2221 ctgtacgttc cagtcggtga ccggcggtgg cctgttctac gtcgacgacg ggccgggcaa
     2281 cagttggcgc ggacgcatca gtggcggtaa caaccgcatc aacgactggc agagcgccag
     2341 ccccatggtg gtggtcaacg ccccgcaagc gcccttctac gcgcgcctgt tgcccgtggc
     2401 cgggccggct ccattggggt tggtggccgc ggcgacgacg gtggtcagta gcaactacga
     2461 cgcctacgag atcagcaccc cgggcgaaat cagcgccctg cactggtgga gcgcggacgg
     2521 catcgccgac ccgctcttca gcgggacgat ccgtctgacg gcggtgaccc ctttcgccct
     2581 cctggccagc ggcaacattc gcctggccgg cggggcggcc cgccgcgatg tggccgccgg
     2641 gacgacggtg cggctggcct atgactctga gcagggtgta tggagcgagg tggcctagcc
     2701 agcagcggcc gccgaccacg attctcagtt ctggtagcta tgtccgagag gcagcgcgtt
     2761 catgagattt acgcccgtcg ccaggggcac agcgagaggt actcgctggg caaccccgcg
     2821 cacctgttca tcatgcaggg gcgacagcgg gccgtcctgc ggcgcttgcg gcgcgagggg
     2881 ctgtggccgc tggccgggca cgacattttg gaggtcggct gcggctccgg cggcgtgctg
     2941 ctggagctat tgacctatgg agccgacgcc ggacggctgc acggcacaga cctgctgggc
     3001 gagcgcgtcg ctgtggcccg gcgacggctg ccccacgtgg ccgtcacttg cgccgacggc
     3061 cgcagcctgc cttaccccga ccaatccttc gatctggttc tgcaattcac gatgttttca
     3121 tcgattttgg atcaggggct ttgctatact gtcgccaatg agatgcgacg ggtattgagg
     3181 ccaggcgggc tactcctgtg gtacgacttc tggctcaacc ccacaaaccg acagacccgg
     3241 ggcatccgtc cgcgcgaaat ccggcagtat tttccggata gccagtacgt tttcgagcgg
     3301 atcaccctcg cgccgccgtt gacgaatcat cttgtaccgt tttcatgggt ggcggcgctc
     3361 agcctggaga ggttgggcat ctttaacacc cactacctgg cagccatccg gcccaaccgg
     3421 tgagttatga acgagagcat cgcttcgcaa ccagtgattg acgccaagct ccgcagcggg
     3481 ctaaccgatc tgcggcatac ggtggacaac atcgcgcggc gcacgctcga cgtgaccatc
     3541 gcggcgctgg gcgtccttgt cctcttgcct ggtctcgtct tgctggccgt gctcatcaag
     3601 cgcgattcgc cggggccggt cttctattgg ggcgagcgcg ccggccgcca cggccggccg
     3661 ttccgcatcc tgaagttccg cacgatgtac gagacaggcg acagctacaa cggcccgcgc
     3721 gtgaccggcc agggcgaccc acgcatcact ccctttggcc aatggctgcg cgagacgaaa
     3781 gtcaacgaac tgccgcagct ctggaacgtc ctcaaggggg acatgagcct ggtggggccg
     3841 cggccagaag accgcgattt cgtcggccac tggccggccg atgtgcgcga ggtgctgctc
     3901 tccgttcgcc ccggcatcac cagcccggcg tccgtcctct atcgggacga ggaaagcatg
     3961 ctctcgtcgg gcaacgtggt cgatcaatat ctgaggatca ttctgcccag caagctgcgc
     4021 ctggacctca tctacgtcca ccaccgctcc ttcctgaccg acctggacgt gttgttctgg
     4081 acgctgatga ccctgctgcc gcggctgaag acgcggcagg tgccggagca cctgctgttc
     4141 tgggggccgc tggcgcgttt catcagccgc tacatgtcct ggttcgtcct cgacagtgtg
     4201 gtggccttcg tcgccgtggc ggccacgggc atcatctggc gcagcaacgg cccgctggag
     4261 cttggcccca gcctggcgct gggcgtggcc ctggccatcg ccctcatctt tagccttgtc
     4321 aacggcgtgc tcggcgtcgg ccgcatcgtc tggtcgcgcg ccgactccga agacgccatc
     4381 accctggcca tctcctccgg cctgacgacg gccattctgc tggccaccaa ctggttatgg
     4441 cagccgcggg ctatccctgt gccctatctg ggccaccgcc tgctgccgtc cggcctgatc
     4501 gtcgtcagcg gcctgttggc ctttgccggc ttcctgctgc tgcgctaccg gatgcgcgtc
     4561 atcaccggcc tgtcgcagta ctggatggcc ctgcgcggcg gcacactggc cgtcggcgag
     4621 cgcgtgctca tcatcggcgc gggcgaggcc ggcaacatgg ccgcctacct gttgcgcaag
     4681 ctggaagccg gccaggcctt ttccatcatt ggcctggtgg acgacgcgcc gcgcaagctg
     4741 ggggcgcgcg tcggcggcct gcgcgtgctg gggccgacgg ttgacgtgcc ccaactggtg
     4801 cgcgtccacg acatcggtct gatcctcttc gccatcgaaa acatccgccc ggccgagcgc
     4861 gagcgcatcc tgcaaatctg ccgcgcgacc ggcgcgcaga cggtcgtcct gcccgacgtg
     4921 ctggcgtcgt tgcggacgca gctggccaac agcgcgccgg ccgcgccggc cgcgccgatg
     4981 cccagcggcg cggaaatgga tagctggctg cgccaactgg atggcctgat cgccgccgaa
     5041 gcttgggacg cggtgcgcga acaggtgcgg acgcggcaac ggcagattgt cagcgactag
     5101 ccgccgcgaa caacagggcc gaccagtgcc caacggcgcg tgttcgcccg gctccccggc
     5161 cgggccgcgc ccgacgacaa gtaaggagta gcgaaccgac atgagcgatt ggaccggcaa
     5221 gcgcgtcctg gtgacgggcg cgggcggttt cattggcagt cacctgaccg agtctctggc
     5281 gcgggccggg gccggcgtgc gcgccttcgt gcgctacaac tcgcgcggcg acgccgggct
     5341 gctgcgccag ttgccggccg aggcgctggc cgaggtcgag atcgtcgccg gcgatctgcg
     5401 cgacggggcc gccgtcgagc gcgccgtggc cggctgcgac gtcgttttcc acctgggggc
     5461 gatcatctcg atcccctact cctatcgcca cccgctggag acggccgaaa ccaacttcat
     5521 gggcacgctg gccgtcctgc tggcctgccg cgcccacggc gtcgagaggc tggtgcacac
     5581 ctccaccagc gaggtctacg gcacggccca gttcacgccg atggacgaga gccacccgct
     5641 gcaaggccag tcgccctact cggccagcaa gatcggcgcc gacaagctgg tggagagctt
     5701 ctaccgctcc tacgacctgc ccacggtgac ggtgcggccg ttcaacactt acggcccgcg
     5761 ccaatcggcc cgcgccgtca ttccgacaat catcacccag gcgctgacgg ccgattgcat
     5821 ccgcctgggc agcctgacga cgcggcgcga cttcacctac gttggcgaca ccgtggccgg
     5881 tttcctgcgg gcggcgctgg cccccgacgt ggccggcgag gagatcaacc tgggcacggg
     5941 cgacgacatc gccatcggcg aactggccga gcgcgtcatc gccctggttg gccggccagt
     6001 ggccatcgtc accgaggcgg agcggctgcg gccggagaag tcggaagtgc ggcgtctgct
     6061 atcgggtaac gaccgggcgc ggcgggtact gggctggcag ccggcgtggt cgctcgatga
     6121 cggcctgcgc cagacggtgg cgtggatcgg cgaccatctc gatttctacc gcgtgggacg
     6181 ctacgaagtc taggagggta tcatgaaagc agtcattctg gccgggggca agggtacgcg
     6241 cctgaagccc tataccacca ttctgcccaa gccgctcatg cccattggcg acatgcccat
     6301 cctggagatt ctgctacgcc agatgcggcg ggcgggtgtg gacgaggccg tgctgaccgt
     6361 gggtcatctg gccgagatgc tgcgcctgtt cttccaggat gggcggcgac tgggcatgga
     6421 cattcgctat agttatgagg acgccccgct gggcacggct gggccgctgt cgttggtcga
     6481 ggggctggac gacacctttc tggtgtccaa tggcgacgta ctgacgaccc tgccgctggc
     6541 cgagctgctg gcccaccatc acgacagcgg cgcggcggcc accatcgcca tgcaccgccg
     6601 ccaggtctac atcgacctgg gcgtcatcca gcaaaacgag gccggccacg tgaccggcta
     6661 catcgagaag cccacctatg acttcatggt cagcatgggc atttacgtct tcgagccgcg
     6721 cgtgctcgac ttcatcccgc gcaacgccta cctcgacttc cccgacctgg tcaagcgcct
     6781 catcgccgcc ggcgagatcg tcagcggctt cccgttcgac ggctactggc aagacctggg
     6841 ccgcgccgac gactacgagc gcgccgtgga agatttcgac cggctgcgcg acgcattcct
     6901 gccgccggag ttgcccgtgg cagccagcgc caacggtgcg atcagcgcgc gcaacgggcg
     6961 aggagggtag gggcatggcc gagaccaacg ggcagtggcg cgttcccctg gccgacatcg
     7021 acctggggcc ggaggaagag gcggccgtgc tggccgtgct gcgccggcgc tggctatcga
     7081 tgggcagcga gacggccgcc tttgaggccg acttcgccgc gctgaccggc gcggcccacg
     7141 ccctggccgt caccaactgc accgccgcgc tgcacctggc tggcctggcc ctcggctggg
     7201 ggccgggcga cgaggtgatc gtgccgtcgc tgacgttcgt ggccacggcc aacgccgtcc
     7261 gctataccgg ggcaacgccg atcttcgccg acattcgcag cgaggacgac ctcagcctgt
     7321 cgcccgacga cgtggccgcg cgcatcacgc cgcgcacgcg ggccattgtt gccgtccact
     7381 acggcggcta cgccgctgac atgcccgcgc tgctggccct ggccgagcgg cacgggctgg
     7441 acgtggtcga ggacgtggcc cacgcgcccg gcggcagcct ggacgaccgc gctctgggca
     7501 cgtgggggcg cgtcggctgc ttcagcttct tcgccaacaa gaacatgacc accggcgagg
     7561 gcggcatgtt gaccaccggc gacgacgccc tggccgagcg gctgcgcctg ttgcgctcgc
     7621 acggcatgac cacgctgacc tgggaccgcc accgcggcca cgccttcagc tatgatgtcg
     7681 tcgcgccggg ctacaactac cggatcgacg agctgcgggc ggccattggc cgggcgcaac
     7741 tggccaaatt gccggccaac aatgggcggc gggcagccat cgacgccgcc tatcgcgccc
     7801 agttgccggt cgccgcgccg gagctggggc tgccctatgc ccgcgcgggg ggcgtgtcgg
     7861 cccaccacct gcggccggcg ctgttgccgc cgggcatcga ccggacgcgg ttcatgaccg
     7921 agatgaaggc ccgcggcatc cagaccagca tccactatcc gcccatccat ctcttcagcc
     7981 tctaccggcg cgacggcggc ccatcactgc ctcttacgga agccgtggcc gcgcgcgagg
     8041 tgacgctgcc gctctatccg ggcatgtcat cgggcgacgt ggagttggtc gtggccgcga
     8101 cggctgaggc gttggccgcc gcgcgggtgg acgcaactgc atcagcaagc gaagttgccg
     8161 ccggaggccg ggccaactga cccgggcaag gccgtttttc accccttttt tcctcttgaa
     8221 tcggggcatt cgatgtaagc tgtagcggta gaaaactgta tcgacagggt agtacgggta
     8281 tggaactcaa actcattctt cagttggtgc gacgctgggc ttggctactg accatcggtg
     8341 tgctggtcgg cggcggcatt ggctatgtcc tcagcatcta tcagacgccg gagtacgagg
     8401 cgcgagccaa gattctggtg atggaaccgg tgcgcaacaa cagcgcctcc acctccacga
     8461 ccttgaaagc caacaaggaa atggccgata cctatctgga gttgctcttc acgcagccca
     8521 tcctcgacgc caccagccag aaggtcggag cgacggtgca cacctcccag attcacgccg
     8581 accgcgttgg cgagacaggc ttgctggagg tcgtcattcg tgacaccgac ccggagcgag
     8641 ccgcgctgac cgccaacacc cttgtccagg tgctcatcga ccagaatgag gccctgcaat
     8701 cgtcgcgctt ctcgtcgtcg gagggcagct tgcaggcgca ggtggccgag gtcgagcagg
     8761 agctgtcgaa cctgagcgac caggtcgaaa agcgctccga ggagactgtc gccgcgcaaa
     8821 cggagcagat cgaaacccag aaggtcgcgc tggaacagca gattaacgcg ctgctgggcg
     8881 agatcacccg gttggagcag gacattgacg acctgacccc taaggttatc gtggccggcc
     8941 agccaccggc ggcgctgacg ctagagcagc gcaacctgct gaccgcgaag cgcaccgaac
     9001 tggcccagaa gcagttccaa ttggaactgg cgcagaataa ctacgccgag cttctcacgc
     9061 cgctgccgag tacgggctcg gccgaggact ctaccaacct gcgcgacatc gagcaggcca
     9121 atctggaact ctatcgccag ctctactcca cgctgttgag caactacgag gccgtccgtt
     9181 tggcccgtct ggagaacacg cccaacgtcg tgcaggtgga gcgggccgcg ccctcgaatg
     9241 tcccggttca atcggggccg ctgcgctaca tcatcatggg cgcgatcatg ggcttgctgt
     9301 tgacgggcac actggccttc acgctggagt acctggacga tacgctgaag acgccgcaag
     9361 acgtggccaa tgtacttggc ctgccggttg tcggctatgt ggttaacgaa gcggggattg
     9421 aaaagggcga gggcacaccc tacgtcagtt ctaacccacg ctcgcccctg gcggagacgt
     9481 tccgcacgct gcgcaccaac ctggagttcg ccagtgtcga caagccgcta aagaccattc
     9541 tcatcaccag ccccggttct agcgagggca agacgacggt ggccagcaac ctggcggcag
     9601 tgatggccca ggccaacaag cgggtcatcc tgctggaggg cgatctgcgc cggccgcgcg
     9661 tccatcgcgc tctgggtatg tcgaaccaga tcggtctgag cgacgtcttc cgcggccaga
     9721 tggacattcg tgatgtggcc cgctactcga aggtgaagga cctggccgtc attaccagcg
     9781 gcagcctgcc gcccaacccg gctgagttgc tcggctcggc gcgcatgtcc caaatcctgg
     9841 cccgtctggt ggagtcggcc tcggtggtca tcatcgacag cccgccgttc ctcgtcaccg
     9901 acgccacggt tctatcggct aaggtggacg gtgtcctgct ggtcatccaa ccgggcaaga
     9961 cccatgccga agcggctaag gccatgttgg cccagttgaa ccgggccggg gcgcacgtgg
    10021 tcggcgtggt gctcaatcgt gtgccgcgca agaacgccaa ctactacggc tactaccgct
    10081 acgagaacga ctacaccatc gaaagcacgc cggccgaagc cccggccgtc aacggcgacg
    10141 gtcgtcgcgc cggcaagggc ctggcgacga tgttcagcgg caagcccggc aaaatcggtg
    10201 aagaggtcgt atcctgacgg gtgtttcctg acgagtgctt cccacgttgt aacggaatcg
    10261 atgcatcatg cctcaaagcg ccacctatcg gctacccttg ctccgccggc ccggcgcggc
    10321 cggcggcgtc aatcgcacac cgggccagtg cccctttcta gggacgccgg ccgatccggg
    10381 aactgcgctg gccttcccct cggaagccaa ccactgcttt cgttcgcgca ttcccatccc
    10441 cattagctcc atccaccagg agaactactg cctctcccac caatacgaga cgtgccccgt
    10501 ctatcggcaa tatgccacca cagaggagcg ggccgaagcg gcatccgtgc ccgtggccgc
    10561 ggtagcgttg gccgcgccgg aaccgcattg ggccggcgag agcgtcggtt cggccattgc
    10621 gccagccacg accgcggcgg gcatggccct gggcgcgggt cgcccctcg
//
LOCUS       DAOSMQ010000042        10645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6223_length_10645_cov_4.755996, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000042 DAOSMQ010000000
VERSION     DAOSMQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6223_length_10645_cov_4.755996"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK829_02535"
     CDS             <1..248
                     /locus_tag="PK829_02535"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40123.1"
                     /translation="DPVNDRAKKMAENMAHVLATAVRERALAATEILGPTPAFFTRLD
                     GRYRWHIVIYSPDPHRLLDDLHIPPPWVVDIDPESTL"
     gene            complement(256..327)
                     /locus_tag="PK829_02540"
     tRNA            complement(256..327)
                     /locus_tag="PK829_02540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Arg,seq:cct)
     gene            724..>2123
                     /locus_tag="PK829_02545"
     CDS             724..>2123
                     /locus_tag="PK829_02545"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOU40124.1"
                     /translation="MKRIAVFTSGGDAQGMNAALRAVVRTGLARGLEVYAIYEGYAGM
                     LQGGEAFKPMTWDDVGGILHLGGTVIGSARSKEFRERAGRLRAARNLLLHEIDGLVVI
                     GGDGSLTGADLLRREWAGLVQELVDTGQVSKKMAARQTALAVVGIIGSIDNDMFGSDI
                     TTGADTALHRIIEATDAISSTAASHQRTFVVEVMGRNCGYLALMSAMASGADWVLIPE
                     APPDVDDWEAKMCEVLMKGREVGRRDIMVIIAEGARDRNGKPITTEHVKKILEDRLHE
                     DTRITVLGHVQRGGAPSAFDRNLSTLLGAEAIEAITSMDPNGEPLIVGMQGNKLTRTR
                     LDVALAKTKAVADAVAAKNYDEALELRGHSFAESFDIVRTLVRALPHEPTPGQRRLRL
                     AVMHGGGPAPGMNTAVRAAVRIAIDRGHTVLGVRNAFRGLINNEIEEMEWMSVNGWAT
                     RGGSELGTNRAIPKGR"
     assembly_gap    2124..2223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2224..3116
                     /locus_tag="PK829_02550"
     CDS             <2224..3116
                     /locus_tag="PK829_02550"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOU40125.1"
                     /translation="TRGGSELGTNRAIPKGRDLYAIARHLEEHKVDGLLLVGGWSGYE
                     SILELYGQRRNYPAFDIPMICLPATINNNLPGTELSVGADTALNSIVSAVDKIKQSAV
                     ASQRTFIVEVMGYYCGYLALMSALATGAERVYLHEQGIHMRELVEDVEAFKRGFRRGK
                     RLGLIIRNEKANEIYTTNFMRTLFEEEGGDLFDVRQAILGHMQQGGTPSPFDRIFATR
                     LAVRCIDFLEQAIGGKESTGVCIGQVQGDIVYTNLDDVPRLMDYEHSRPLKQWWMDLR
                     PIAHVLASPGISANDEENRG"
     gene            3188..4354
                     /locus_tag="PK829_02555"
     CDS             3188..4354
                     /locus_tag="PK829_02555"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOU40126.1"
                     /translation="MKIGILTGGGDVPGLNPCIKAVVNRAVDHGHEVVGIRRGWGGLL
                     LVNRDDPQSIEQYIMPLSHNEVRTIDRTGGTVLHTSRTNPGKVKPNDVPDFLKDPEHP
                     EAEAADKSTRDFTPHILKNLEYLQIDRLIPIGGDDTLSYGERLHREGFPVVAIPKTMD
                     NDVYGTDYCIGFSTAVTRSVQFIHQLRTSAGSHERIAVIELFGRNSGETSLISSYLAG
                     VERALISEVPFDPERLAALVMEDKRTNPSNYAMVTVSEGAHLSGGAIVEFGEADAYGH
                     RKLGGIGDITGDALKRLTGENIISQKLGYLMRSGAPDALDLMVGTNYAHLAVDLIVSG
                     SSGRMVALRDGAYTHVAMSMVTSGVKRVDVDELYDVANYRPKVRHVLGKPMFLY"
     gene            4557..5750
                     /locus_tag="PK829_02560"
     CDS             4557..5750
                     /locus_tag="PK829_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU40127.1"
                     /translation="MARFLRSSPARLIVLLALLLLAACKGSLTDEGRTATPDQAVVVQ
                     QAVATITAQMATTAPNATLAPAQLPAITTDLEADLIALYEHINPSVVHIFVYDTADNP
                     VGTGTGFVFDDRGHIVTNNHVVAGAGRLEIVFPNGERRTGLTVGTDVDSDLGVVLVDS
                     LPDGVTPIPLGDSDAVRVGQFVVAIGNPFGEAGSLSLGIVSALGRTLTSDRITEGGGR
                     YSLPQVIQTDAAINPGNSGGPLLNLAGEVIGVNSSIRTSTGTNSGVGFSIPVNAVRRI
                     APALITNGTYHYTYMGIRMAALDLTSQQALGLPQVTGAYVTEVSPNAPAALAGLRAGG
                     VDEVGELLPGGDLIIAVDGQPIASSDDLISYLVFSTEVGQTINLTIIRDGQQLDVPLT
                     LGQRP"
     gene            5833..6951
                     /locus_tag="PK829_02565"
     CDS             5833..6951
                     /locus_tag="PK829_02565"
                     /inference="COORDINATES: protein motif:HMM:NF037526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40128.1"
                     /translation="MFSQDDMQELLTYEGNGDGVVSIYLDTDSARQSTEAIKLTAKGL
                     LKEIQPQHERESQTIERYLDLSYDWSKPGLALFCGQGGSYFRSYPSPVAFRNRIRLGP
                     RPYVKPLAHFLDHYAHFGVIAVDRVNGRFYEYHLGEPQSSESFSGDEVRKVKRGGGSS
                     AVGRRGGGGASGAGGGDHEEEVAARNLRDCAAAAGRFFAARPIRRLFLGGTNETVAQF
                     RELLPKQLQTCLAGSFAMDMDMGEPEVRRRALELLREVNAEREAQLVDRLTTLKAMGA
                     NAVVGLDDTLQAVTDKRVEALIISDGFRHHGYIDEASGFVVANLARSPLAENELTAVE
                     DVVDVAVAQTVAQGGHVEIIADSPALEDSGRIGAILRY"
     gene            6995..7654
                     /locus_tag="PK829_02570"
     CDS             6995..7654
                     /locus_tag="PK829_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU40129.1"
                     /translation="MAAPVRFVAVAVDAIPAFVARLADMPAPPHARNPYAGDGPAAVR
                     RDNLTLYLTRLAARRPWALLVGEAPGYRGCGATGVPFTSAVILLAEPCPFGLFGAAAG
                     FRLAEDGRPAREATATILWDTLRARDHLPLLWNVCPFHPHRPGRPFSNRAPAAAEIAA
                     GEWAVRELLALFAVEQVIAVGRVAGTALGRWGIAATTVRHPSHGGKAAFGQQLATVPG
                     R"
     gene            complement(7635..9281)
                     /locus_tag="PK829_02575"
     CDS             complement(7635..9281)
                     /locus_tag="PK829_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40130.1"
                     /translation="MTPDTGFRDLRAYLAHWDDRRRLQTLLDWLPRALAAGLLPGLGL
                     ALLARMRPLLTAAEIALAALAIAVMAVAVAVLIVLVSRRSLAEQARFADRRFALRERA
                     TAAVEIRAGQHDVPPELAARQLGDAVAAAAAVDTARQMPLTTGLRDWLPALLALAALL
                     LALGLPNPQQLLLLQQRAVAAAMGEQAAALESLSEQIGADASLSPSQQAALQRPLEEA
                     LATLNEPGVSPAEAMAALSAAEAELRSLTQEFDEAAASASVAAAADALSRSEQTADFA
                     AALEAGDANAAADAVAGLADRLPQMDAPARAELAQSLADAADAMRQVSPGAAAELDRV
                     ADALAAGDIAEAQAALDDMAASIEEAGTAARAAARAEQAAEAIGQSRQSVAAAGAEDT
                     AGDTQAATGAAGTGEQAGSGGQTGGEGQSSGSGQQGNGQEGQGAAGQGQGSQGTATGG
                     PSEGGGHVENVFVPAPVDLNGQGENVELEVQCLSDPAACGPQGEPLPNSPDLPTGGSF
                     VPFDQVFGDYRDAAFEALPGSGVPPALQGLVRDYFSALEP"
     gene            complement(9278..10174)
                     /locus_tag="PK829_02580"
     CDS             complement(9278..10174)
                     /locus_tag="PK829_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40131.1"
                     /translation="MSAWLSNPVAIKELRGRMRGRRAFVVLTLYLLALSGIVTFTYLI
                     YSASAAIPGSTNARTTGKALFAAILAVQVFLVAFIGPAFTAGAISGERERQTYDLLRT
                     TLLPAESFVRGKLISALSYVFLLVLVSIPLQSIAFLLGGLSAVELIVSQVLILVAAVT
                     YALYGLWCSAAMRSTLAATVATFAGMLFLLFGIPLIGGAAALFLNLWFATAGSGGGPL
                     TETLALYAGFISAATNLPATLLVSEALFIEENAIFATSQTIGSTRVWLPLPWPLFILF
                     HLLVAYWLYRATVRRVRRVSDK"
     gene            complement(10171..>10645)
                     /locus_tag="PK829_02585"
     CDS             complement(10171..>10645)
                     /locus_tag="PK829_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HOU40132.1"
                     /translation="VRELLVEMAHMGKTIFFSSHILSEVADICTSVGILEHGHLVASG
                     SVAEMLSGLRGHRSVAVRLLAGAADLAEADAWLRGQPGVVAVSGPRTDAPPADGHADG
                     HADGHTLRLDFSGDDAALSALLAGMVSRGLPVVAFGEEQGDLEDVFMHLTRGPGT"
BASE COUNT         1773 a         3543 c         3480 g         1749 t
ORIGIN      
        1 ccgacccggt caacgatcgc gccaagaaga tggccgagaa catggcccac gtgctggcca
       61 cggccgtgcg cgagcgagcg ctggcggcca cggagattct cggcccgacg ccggccttct
      121 tcacacggct cgacggccgc tatcgctggc acatcgtcat ctactcgccc gacccccacc
      181 gcctgctgga cgacctccac atcccgccgc cgtgggtggt tgacatcgac ccggagagca
      241 cactttaggt ctcattgtcc ccgcacggag tcgaaccgtg atcgatcgct taggaggcga
      301 ttgctctatc ctttgagcta cgaggacgcg cctcgaattc tagcctagcc atcccccagc
      361 ggccacctgc cagcatggct cttgagtagt cgcccgttgg ccaaagaccg gcacaggtcg
      421 gcagcccgac ggctcatttg tgctggagcg atttcggccg cccatctgtc agatcgacgc
      481 gttccgttaa taaaacacga aaaactcctt gcctcttgcg tcgaaaatag tactattcca
      541 ttcggcattc ccacacccca gatgcaagaa ccactgcacg ctgacagccg cacatgaggc
      601 tcgaagacgg gtcacattga ctcgcttccc cctttcgcta cgttgccccc gcggcccaat
      661 cagtcagcac gttcccgtgc accctggcgc aaaccaacgc agatgaggat ggtatatccg
      721 gtaatgaaaa gaatcgctgt tttcacgagc ggtggcgacg cccagggcat gaacgcggcc
      781 ctacgcgcgg tagtgcgcac ggggttagcg cgcgggttag aggtctacgc catttatgag
      841 ggctacgcgg gcatgctcca gggcggggag gccttcaagc ccatgacctg ggacgacgtg
      901 ggtggcatcc tccacctcgg cggcacagtc attggatcgg ctcgcagcaa ggagttccgc
      961 gagcgcgccg gccgcctgcg cgcggcgcgt aacttgctgt tgcacgaaat cgatggcctg
     1021 gttgtcattg gtggcgatgg cagtctgacc ggcgcggatc tgcttagacg cgagtgggcg
     1081 gggctggtgc aggaactggt cgacaccggc caggtcagca aaaagatggc cgcgcgccag
     1141 actgcgttgg ccgtcgtggg catcattggc tccatcgaca acgatatgtt cggctccgac
     1201 atcaccaccg gggccgacac ggccttgcac cgtatcatcg aggctaccga cgccatcagc
     1261 agcaccgccg ccagccacca gcgcaccttc gtcgtcgagg tcatgggccg caattgcggc
     1321 tacctggccc tgatgagcgc catggcttcc ggggctgact gggtgctgat tcccgaagcg
     1381 ccgcccgacg tggacgactg ggaagcgaag atgtgcgaag tgctgatgaa gggacgcgag
     1441 gtcggccggc gcgacatcat ggtgatcatt gccgagggcg cgcgcgaccg caacggcaag
     1501 cccatcacca cggagcacgt caagaaaatc ctggaagatc gcttacacga ggatacgcgc
     1561 atcaccgtgc tgggccacgt ccagcgcggt ggcgcgccca gcgccttcga ccgcaacctg
     1621 agcacgttgc tcggcgcgga agcgatcgag gccatcacgt cgatggatcc caacggcgag
     1681 ccgctcatcg ttggcatgca gggcaacaag ctgacgcgca cgcgcctgga tgtggccctg
     1741 gccaagacca aggctgtcgc tgacgctgtg gccgccaaga actacgacga ggcgctagag
     1801 ctgcgcggcc actcgttcgc cgagtccttc gacatcgtgc gcacgctggt gcgggcgctg
     1861 ccccacgagc caacgccggg gcaacgccgg ctgcgcctgg cggtgatgca tggcggcggc
     1921 ccggcgccgg gcatgaacac cgccgtgcgc gccgccgtac gtattgccat cgaccgcggc
     1981 cacaccgttc tcggcgtccg caatgccttc cgtggcctga tcaacaacga gatcgaagaa
     2041 atggagtgga tgagcgtcaa cggctgggcc acgcgcggcg gctcggagtt gggcaccaac
     2101 cgcgccatcc ccaaggggcg cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnccacgcg cggcggctcg gagttgggca ccaaccgcgc catccccaag gggcgcgacc
     2281 tctacgccat tgcgcgccac ctggaggagc acaaggttga tgggctcctg ctggtcggcg
     2341 gctggtcggg ctatgagtcc atcctggagc tatatggcca gcgtcgcaac tacccggcct
     2401 tcgacatccc gatgatctgc ctgccggcga cgatcaacaa caacttgccg ggcacggaac
     2461 tgagcgttgg ggcggacaca gcgctcaaca gcatcgtttc ggccgtggac aagatcaagc
     2521 agtcggccgt ggccagccag cggacgttca tcgtcgaggt catgggctac tactgcggct
     2581 acctggccct gatgagcgcg ctggctaccg gcgcggagcg cgtctacctg catgagcagg
     2641 gcatccacat gcgtgaactg gtcgaggatg tcgaggcttt caagcgcggc ttccggcgcg
     2701 gcaagcggct gggcctgatc atccgcaacg agaaggccaa cgagatctac acgaccaact
     2761 tcatgcggac gctgtttgag gaagagggcg gcgacctgtt cgacgtgcgc caggccattt
     2821 tggggcatat gcagcagggc ggcacgcctt cgcccttcga ccgcatcttc gccacccggc
     2881 tggccgtccg ctgcattgac ttcctggagc aggccatcgg cgggaaggaa tcgaccggcg
     2941 tctgcatagg ccaggtgcag ggcgacatcg tctacaccaa tctcgatgat gtgccccggc
     3001 tgatggacta cgagcacagc cgcccgctca agcagtggtg gatggacctg cggcccatcg
     3061 ctcacgtcct ggcttcgccg ggcatcagcg ctaacgacga ggaaaaccgc ggctgattcc
     3121 cggctctggc cggcggccgc ctttcgtttg cattccagca ttcatcttat caacgaggag
     3181 acatagcatg aaaatcggta ttctgacagg cggcggcgat gtgcccggcc tgaacccatg
     3241 tattaaggcg gtcgtcaacc gcgcggttga ccatggccat gaagttgttg gcattcgccg
     3301 cggttggggt ggcctgctgc tggtcaaccg cgatgacccg cagagcattg agcagtacat
     3361 tatgcctctg agccacaacg aagtgcgcac catcgaccgc actggcggca cagtgctcca
     3421 caccagccgc accaaccccg gcaaggtcaa gcccaacgat gtccccgatt ttttgaagga
     3481 cccagagcac cctgaggctg aggcggccga caagtctacc cgcgacttca caccccacat
     3541 cctgaagaac ctggagtacc tacagatcga ccggctcatc cccatcggcg gcgacgacac
     3601 gctcagctat ggcgagcggc tgcaccgcga gggctttccc gtcgtggcca ttcccaagac
     3661 gatggacaac gacgtctatg gcacggacta ctgcatcggc ttcagcacgg ctgtcacgcg
     3721 cagcgtccag ttcattcacc agttgcgcac cagcgccggc tcacatgagc gcatcgctgt
     3781 catcgagctg ttcggccgca actccggcga gacgagcctg atcagttctt acctggccgg
     3841 cgtcgaacgc gccctcattt ccgaggtgcc gttcgacccg gagcggctgg cggcgttggt
     3901 gatggaagac aagcgcacca accccagcaa ctacgccatg gtgaccgtca gtgagggcgc
     3961 gcacctgtcg ggcggggcta tcgtcgagtt cggcgaggcc gacgcctacg gccaccgcaa
     4021 gctgggcggc atcggcgaca tcaccggtga cgccctgaag cgcctgacag gcgagaacat
     4081 catcagtcag aagctcggct atttgatgcg ctctggcgcg cccgacgctc tcgacctgat
     4141 ggttggcact aactatgccc atctggccgt cgatctcatt gtctccggct ccagtggacg
     4201 gatggtggcc ctgcgcgacg gcgcttacac ccatgtagcc atgagtatgg tcaccagcgg
     4261 cgtcaagcgc gtagacgtag acgagctata cgacgtggcc aactatcgcc ccaaggtgcg
     4321 tcacgtgctg ggcaagccga tgttcctgta ttgatccgtc gccagcgcga cgcaacgcaa
     4381 caacgggtgt gggcggcctc tcgacggggc cgcccgcgcc cgtttcgtca tctgatagct
     4441 gcgtaagtgg tcaccgctgc cccttgactt gtcactggac gggattctag aatagacata
     4501 tccttagggt atcggttcgt cttcagtttt ccaccggtca ataagtgatc ctttcaatgg
     4561 cccgattcct gcgctcttcc cctgcccgtc taatcgttct actggctctg ttgctgttgg
     4621 ccgcgtgcaa agggtcgctt actgatgaag gccgcacggc gacgcccgac caggcggtcg
     4681 tagttcagca agccgtggcc accatcaccg cacaaatggc gacgaccgcg cccaacgcca
     4741 ccctcgcgcc cgcgcaactg ccggccatca ccaccgatct tgaagctgac ctcattgccc
     4801 tctacgagca catcaatcct tctgtcgtcc atatcttcgt ctacgacacg gctgataacc
     4861 ccgtgggcac gggtacgggc tttgtcttcg atgaccgcgg ccatattgtg accaacaacc
     4921 acgttgtcgc cggggcgggg cggctggaga tcgtcttccc caacggcgag cgccgcacag
     4981 ggctgacggt tggcacagac gtggacagcg atctcggtgt tgttctcgtg gattcgttgc
     5041 cggatggcgt gacgcctatc cccctgggcg attcggacgc ggtgcgcgtt ggccagtttg
     5101 tcgtcgccat cggcaaccct ttcggcgagg ccgggtcgct gtcgctgggc atcgttagcg
     5161 cgctggggcg cacgctgact tccgaccgca ttactgaggg cggcggtcgc tactcgctgc
     5221 ctcaggtcat ccagacggac gcggccatca atcccggcaa ttccggcggt ccgctgctca
     5281 atctggccgg cgaagtcatt ggcgtcaaca gctccatccg caccagcacg ggcacaaact
     5341 ccggtgtcgg tttctccatc cccgtcaatg ccgtccggcg tattgcgccg gcgctgatca
     5401 ctaacggaac gtatcactac acctacatgg gcatccgcat ggccgctctg gacctgacca
     5461 gccagcaggc gctgggtttg ccacaggtga ccggggcgta tgtgactgag gtctcgccca
     5521 acgcgccggc ggcgttggcc ggcttgcgcg cgggtggcgt agacgaagtg ggcgaattgc
     5581 tgcccggcgg cgacctgatc atcgctgtcg atggccagcc cattgccagt tccgacgatc
     5641 tgatcagtta tctggtattc agcacggagg taggccagac catcaacctg acgatcattc
     5701 gcgacggaca acaactcgac gtacccctga cgctcggcca gcggccgtag gctgccctga
     5761 gcgaccttat cccacaccga ccgtctgccg gcgagctgcc cggcagaacc ggacccatcc
     5821 taggaggaat ccatgtttag ccaggatgat atgcaagagt tgctgaccta tgagggtaat
     5881 ggcgatggcg tggtcagtat ctatctcgac actgatagcg cgcgccagtc taccgaggcc
     5941 atcaagctaa ccgccaaagg gctacttaaa gagattcagc cgcagcacga acgcgagtcg
     6001 cagaccatcg aacgctatct cgacctgagc tatgactggt ctaagcctgg tctggccctc
     6061 ttttgtggcc agggcggcag ctacttccgc tcttatccat caccggtcgc ctttcgcaat
     6121 cgcattcgtt tggggccgcg cccctacgta aagccgttgg ctcacttcct ggatcattac
     6181 gcccacttcg gcgtcatcgc cgtcgaccgc gtcaacgggc gcttctacga gtaccacttg
     6241 ggcgagccgc agtcgagcga gagcttcagc ggcgacgaag tccgcaaggt gaagcgcggc
     6301 ggtggctctt cagccgttgg ccggcgtggc ggcggcggcg cgagtggcgc cggcggcggc
     6361 gaccacgagg aggaggtggc ggcgcgcaac ctgcgcgact gcgcggcggc ggccggccgc
     6421 ttcttcgccg cacggcccat ccggcggctg ttcctgggcg gcaccaacga gaccgtggcc
     6481 cagtttcgcg agttgctgcc caagcaactc cagacctgcc tggccggttc cttcgctatg
     6541 gacatggaca tgggcgagcc ggaagtgcga cgccgggcac tggagctatt gcgcgaggtc
     6601 aatgctgagc gcgaggccca gctagtggat cgcctgacaa cgctcaaggc catgggcgct
     6661 aacgccgtcg tcggcctgga tgatacgctc caggccgtaa ccgacaaacg cgtcgaggcg
     6721 ctcatcatca gcgacggctt ccgccatcac ggctatatcg acgaggcgtc gggcttcgtc
     6781 gtcgccaacc tggcccgcag cccgctggcc gagaatgagc tgaccgcggt cgaggatgtc
     6841 gttgatgtcg ccgtggccca gaccgtggcc cagggtgggc acgtggagat catcgctgat
     6901 agcccagcgc tggaagacag cggccgtatc ggcgcaatcc tgcgttacta gttaacttct
     6961 gttgctgaga cgggcgcggc caccgggtag tgtagtggcc gcgcccgttc gctttgtcgc
     7021 tgtagctgtg gacgccattc ccgccttcgt cgctcgcctg gcggacatgc ctgcgccacc
     7081 ccacgcccgc aatccctacg ccggcgacgg cccggcggct gtccggcgcg acaacctgac
     7141 cctctacctg acgcgcctgg ccgcgcgccg gccgtgggcg ctgctggtgg gcgaagcccc
     7201 tggctaccgc ggttgtggcg caaccggcgt gcccttcacc agcgcggtta ttctcctggc
     7261 cgagccatgc cctttcggcc tgttcggcgc ggcggccggc tttcgcctgg ccgaggacgg
     7321 ccgcccggcg cgcgaggcca cggcgactat cctctgggac acgctgcggg cgcgcgacca
     7381 tctgccgctg ctgtggaatg tctgtccctt ccatccccat cggcccggcc gaccgttcag
     7441 caaccgcgcg ccggcggcgg ccgagatcgc cgcaggggag tgggccgtgc gcgagttgct
     7501 ggcgctgttc gctgtggagc aggtgattgc cgtggggcgc gtcgccggga cggccctggg
     7561 acgctggggc atcgccgcga cgaccgtccg ccaccccagc cacggcggga aggcggcgtt
     7621 cggccagcag ttggctacgg ttccagggcg ctgaagtagt cgcgcaccag cccttgcagg
     7681 gccgggggca cgccactgcc gggcaacgcc tcgaaagcgg cgtcgcggta gtcgccgaag
     7741 acctggtcga agggcacgaa gctgccgccc gtgggcaggt cggggctgtt gggcagcggc
     7801 tccccctgcg ggccgcacgc cgccgggtcg ctgaggcact gcacttccaa ctccacgttc
     7861 tctccctggc cattgaggtc aaccggcgcg ggcacgaaga cgttctcgac gtggccgccg
     7921 ccctcgctgg ggccgccggt ggccgttccc tggctgccct ggccctggcc ggccgcgccc
     7981 tggccttcct ggccgttgcc ctgttggccg ctgccgctgg actggccctc accgcccgtc
     8041 tgcccaccgc tgccggcctg ttcgcccgtg cccgccgcgc cggtcgcggc ctgggtgtcg
     8101 ccggccgtgt cctccgcgcc ggccgcggcg acactctgcc gcgactggcc gattgcctcg
     8161 gcggcctgct cggcgcgggc ggcggctcgg gcggctgtgc cggcctcctc aatgctggcg
     8221 gccatgtcgt ctagcgcggc ctgcgcttca gcgatgtcgc ccgcggccag ggcgtcggcc
     8281 acgcggtcga gttccgcggc cgcgccgggg ctgacctggc gcatggcgtc ggcggcgtcg
     8341 gcgaggctct gggccagttc ggcccgcgcg ggcgcgtcca tctggggcag gcggtcggcc
     8401 aggcccgcga cggcatcggc ggcggcgttg gcgtcgccgg cctctagcgc cgcggcaaaa
     8461 tcggccgttt gctcgctgcg gctcagggcg tcggccgcgg cggcgacgct ggcgctggcg
     8521 gcggcctcgt cgaactcctg cgtcaggctg cgcagttccg cctccgccgc cgatagcgcc
     8581 gccatcgcct cggccgggct gacgcccggc tcgttgagcg tcgccagcgc ttcttccagc
     8641 ggccgttgca gggcagcttg ctgcgacggc gacaggctgg cgtcggcccc gatctgctcc
     8701 gacaggctct ccaacgccgc ggcctgctcg cccatggcgg cggcaacggc gcgctgttgg
     8761 agcaggagga gctgttgcgg gttgggcagc cccagggcca gcagcagtgc cgccagggcc
     8821 agcagcgccg gcaaccagtc gcgcaggcct gtcgtcaggg gcatttgccg ggcggtgtcc
     8881 acggccgcgg cggcggccac ggcgtcgccc aactgccggg cggccagttc cggcggcacg
     8941 tcgtgttgcc cggcgcgaat ctcgaccgcc gccgtggccc gctcgcgcag ggcgaagcgc
     9001 cggtcggcaa aacgcgcctg ctcggccagg ctgcgccggc tgacgagcac aatcaggacg
     9061 gccacggcca cggccatgac ggcgatggcc agggcggcga gggcgatctc agccgcggtc
     9121 agcagcggac gcatgcgagc cagcagcgcc agtcccagcc ccggcagcag cccggcggcc
     9181 agcgcccgcg gcagccaatc gagtagcgtc tgaagacggc ggcggtcgtc ccagtgggcc
     9241 aggtaggcgc gcaggtcgcg gaagccggtg tcgggggtca cttgtcgctc acgcgccgca
     9301 cgcggcgcac cgtcgcccgg tagagccagt aggcgaccaa gagatgaaac aggatgaaga
     9361 gcggccaggg caggggcagc cagactcgtg tgctgccgat agtctggctg gtggcaaaaa
     9421 tggcgttctc ctctatgaag agggcttcgc tgacgagcaa cgtcgccggc aggttggtgg
     9481 ccgcgctgat gaaacctgcg tagagagcca gcgtctcggt gagcgggccg ccgccactgc
     9541 ctgccgtggc aaaccacaag ttgaggaata gggccgccgc gccgccgatc agcgggattc
     9601 caaagaggag gaacagcatt ccggcgaagg tggccacggt ggccgccagc gtgctacgca
     9661 ttgccgccga gcaccacagg ccgtagaggg cgtaggtgac ggcggcaacc aggatgagca
     9721 cctgtgagac gatcagttcg acggccgaca ggccacccag caggaaggcg atgctctgta
     9781 aggggatgga caccagcacc aacaggaaga cgtagctgag ggccgagatg agcttgccac
     9841 ggacgaacga ctcggccggc agcagcgtcg tgcgcagcag gtcatacgtc tgccgttcgc
     9901 gctcgccgct gatggccccg gcggtgaagg ccgggccgat gaaggcgacc aggaagacct
     9961 gcacggccag gatggcggcg aaaagggcct tgccggtcgt gcgcgcgttg gtgctgccgg
    10021 gtatggcagc gctggcgcta taaatcagat aggtgaaggt gacgatgccg ctgagagcca
    10081 gcaggtagag ggtcaggacg acgaaggcgc gccgcccgcg cattcgcccg cgcagttcct
    10141 tgatggccac ggggttgctc agccaggcgc tcacgttccc ggccctctgg ttagatgcat
    10201 gaagacatcc tccaaatcgc cctgctcctc gccgaaggcg acgacgggca ggccgcggct
    10261 gaccatcccg gccagcaggg cgctgagcgc ggcgtcgtcg ccgctgaagt ccagacgcag
    10321 ggtgtggcca tccgcgtggc catccgcgtg gccatcggcc ggcggcgcgt ccgtccgcgg
    10381 gccgctgacg gcgacgacgc cgggctggcc ccgcagccag gcgtcggcct cagccagatc
    10441 ggccgcgccc gccagcagcc gcacagcgac cgaccggtgg ccacgcaggc cgctgagcat
    10501 ctcggccacg ctgccgctgg ctaccagatg gccgtgctcg aggatgccga cgctggtaca
    10561 gatgtcggcc acctctgaga gaatgtggga ggagaagaag atcgtcttgc ccatgtgcgc
    10621 catctcaacc agcagttcgc gcact
//
LOCUS       DAOSMQ010000043        10628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6230_length_10628_cov_4.613071, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000043 DAOSMQ010000000
VERSION     DAOSMQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6230_length_10628_cov_4.613071"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="PK829_02590"
     CDS             <1..1453
                     /locus_tag="PK829_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOU40133.1"
                     /translation="KGGTFDAAAHHALARRVAGGSMVLLKNNGLLPLQAPQRIAVIGR
                     AALEAHFQGGGSSHINPTQVDVPFEELRRLAGDAEFRYAAGYPDDDSRDQALIDEAVA
                     AAQATDVALLYIALPTFKESEGYDRPDMGLTTQQVALIQAVTAAQPRTVVILNNGAPV
                     AMSEWIDGAAAVLEAWMMGQAGGGAIADILFGRVNPSGKLAETYPLRLVDTPAFLNFP
                     GENGDVRYGEGLFIGYRYYDTKQMPVQFPFGYGLSYTTFAYDHVRVSAGNGVPTVHFR
                     DVDGVTVSVDVTNTGAVAGKEVVQVYVHDRAARLIRPAKELKGFAKVALEPGETRTVN
                     IPLDFRAFAYYDPAYHQWITEDGEFDILVGASSADIRGQATVTLECTLELPSLLHDES
                     TIRAWFADPIGQPILQPIFDELMKKGGVFAGNGSEEPVDMDLLSFLMDLPLRSFFHFQ
                     EGYLTQPPDDITNMLLEQVHGLGSRKLALAPAL"
     gene            1687..2721
                     /locus_tag="PK829_02595"
     CDS             1687..2721
                     /locus_tag="PK829_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40134.1"
                     /translation="MTDQQPPPPPRRRSARRVARKATPTNSPGLGLAALVVLLATLPL
                     LGILYLGAQLLRLPFPPLDLYDWPVRAGFTPWAALVDSLSSPTGNIAETAPLVQWLVG
                     LALFLALAYLVGLAFYAFVMRRGRAPDAIDGLTAGALLAVPMVFVSLAVRSSPLPAPL
                     IIVWLVAALVGWGLLLSYAFGRLLRPAPAVVAPAMLPSEEPVTPIAPAAATTPTPAGI
                     DRRRFLLQFGASTAAITAVTTAAGAALARREDAETLQRTLPMISPEFRAAQQELFGRF
                     RRFAIVRGAADSVDKTNVLALGAEYPDRNYVSIWLGGHSPIVIYENLETALAAYSAEG
                     EQTGLFWLDS"
     gene            2941..3381
                     /locus_tag="PK829_02600"
     CDS             2941..3381
                     /locus_tag="PK829_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40135.1"
                     /translation="MVNRQTPSPISPDLLEILRDPQAIQEGDRYGADPGRLELVRGCW
                     LVSADTGYKYPIRDGIPVMLIEEGARWKDTPVAALPVPPPPAIDAPAPETRADEVATM
                     PAANETNIALLVAIGALVLALLIVIGLSRRHAASPEPLPEPLVP"
     gene            3378..5111
                     /gene="aceK"
                     /locus_tag="PK829_02605"
     CDS             3378..5111
                     /gene="aceK"
                     /locus_tag="PK829_02605"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HOU40136.1"
                     /translation="MTRLTDSRLANVGAGTLQNAYNTFHDQFQAITGQAKHRFEMQDW
                     GGLQEDAAARLGLYRRIVELAEAAIRDLLRERLDNKIIWASMKAVYSSLIAQRDDWEV
                     AETFFNSVTRRIFTTVGVDHQIEFVATDFETPPTRPQSPVYRVYSRITSTTDLVMAIL
                     RDSRFAASFRDMAEDAHLAAARIDAQLEALNTLHTVQRVEMVKPAFYRGMGAYLVGRL
                     FSGSQSFPLALALIHTPEGITVDSILLDEDSVSILFSFAHSYFHVDVARPYDLVHFLK
                     TIVPRKRTGELYISIGYNKHGKTELYRDLLDHLAYTQERFEIARGQRGMVMLVFTMPG
                     YDIVLKLIKDHFNYPKDSTRKEVMAKYDLVYRHDRAGRLVDAQSFEYLEFSRDLFSPE
                     LLEALQRDCSQSVVIGQKTVVLAHTYFQRRVIPLDIFTREAREAAACAAVVDYGQAIK
                     DMACSNIFPGDMLLKNFGVTRHGRVVFYDYDELCLLEECNFRRLPPTDRYEEELAAEP
                     WFHINTNDIFPEEFPNFLGLSGSLRAVFESAHSDLYTVDYWRARQEAIRGGERAHIFP
                     YQAGQRLHQGV"
     gene            5115..6113
                     /locus_tag="PK829_02610"
     CDS             5115..6113
                     /locus_tag="PK829_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU40137.1"
                     /translation="MEYRRLGKSGLPVSALSYGSWVTFSHQLGVEQALETMSAAYEAG
                     VNFFDNAEVYAGGRSETIMGQALRQVGWPRDSYIVSSKVMWGSVDDPKPTQRGLHRKH
                     VFEACHDALRRLQVDYLDLYFCHRPDINVPMEEIVRMMTELIHQGKVLYWGTSQWSAQ
                     QLAEAYGVARQYHLIPPTMEQPQYSMLHRDKVEREFLPLYETMGLGTTTFSPLYFGIL
                     TGKYNDGIPTDSRANLAGYEWMRDNIESDEGQANLEKVRRLTPIAEGLGTTMSRLAIA
                     WCLRNPHVSTVILGASRVEQVHDNLHALELLPQLTDDVMAAIDEVLANKPAAPETY"
     gene            6135..6878
                     /locus_tag="PK829_02615"
     CDS             6135..6878
                     /locus_tag="PK829_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU40138.1"
                     /translation="MSQRLLPITGLPVGEVTPAVIVCGDPARAARIAARLDEATPLSH
                     HREYRALRGGYEGLPLTVCSHGVGAPGAAVAFEELIAAGARQIVRVGTCGALQAGMDA
                     GHLVVATAAVQNIGYGRETVPDGYPAVADPTLTLALARAATEAGPPAHSGFVISRDSF
                     YPGVPLAGAPDYRVLSAARVLAVEMECAALFLVGSLRGVATAAILAVDGNVLASAESM
                     DSYQPGQEVVGVAVEAAIGCALRALRSVA"
     gene            6875..7627
                     /locus_tag="PK829_02620"
     CDS             6875..7627
                     /locus_tag="PK829_02620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40139.1"
                     /translation="MTGIQDKRRAIRHLLREREPADAMAAYYALYHPDERTTLIVSPL
                     VDGQHADGYVALSRTGIDLFRPLVTMRLPTDRDPTATEAAAALLRQALGPGQPVIISA
                     PDRYLPLLRALGDTQSEERLRLYALDGRRFEPVINVLVTRGEGPGGLPRFTIRSSQTG
                     TEEVAATASLNWQSPTFAEIAVSTRPQFRRQGWGRSVVAALCQYVLESGRTPLYVVAE
                     ENAASIQLAESLGFADTGAREAFLQTMLRPIS"
     gene            7954..8166
                     /locus_tag="PK829_02625"
     CDS             7954..8166
                     /locus_tag="PK829_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOU40140.1"
                     /translation="MFRASGIVKWFSRLKGYGFINPDEGGQEVFVHYSAIDGDGYRNL
                     YEGDRVEYDLIDQGKGPQAKRVVPKR"
     gene            8297..8674
                     /locus_tag="PK829_02630"
     CDS             8297..8674
                     /locus_tag="PK829_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU40141.1"
                     /translation="MSHSVLIVDDNLSLANLYQIVFERVGWAVTRVSSGQEALDSIAG
                     RVPDVILLDVMMPVMDGIEACRRIRALRPEKPPCIVIYSANNRPEVRETVLAAGADAF
                     VSKNLSVFELPSHILVYLPEGSR"
     gene            complement(8671..9849)
                     /locus_tag="PK829_02635"
     CDS             complement(8671..9849)
                     /locus_tag="PK829_02635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU40142.1"
                     /translation="MASVSEHEVIVVGAGPGGATAATALAQAGRDVALLDRQRFPRDK
                     ICGDAVSLGAIRIMNDLGMADKIAAARARGEFYPLDGMRLVAPSGHTLHMDFHPGSVG
                     EDSYVAPRLYYDAVIQQHAIDSGACFEQAEVEAPLLVDGRVVGVRARRNGAVHEYRAP
                     LVIAADGVTSTIMRQLRPETEQHVDMHRAVALRAYIEGMEIFPHKVEFFLYEEVLPGY
                     AWIFSAGDGLANVGLGMRLDVFRRNRHNLKKMLHDFLDMPAIRPRLREGHTVRDMAIW
                     QLSFGSQKGLQYAFDGALLVGDAAGFINPLTGGGIHNALISALLAAETADEALRRGDV
                     SRAGLQVYEERCRETMWSGMRTSFFYQRFLMNYPMLVDRLIRFLGRHGGLAKVFVSKL
                     "
     gene            complement(9971..10435)
                     /locus_tag="PK829_02640"
     CDS             complement(9971..10435)
                     /locus_tag="PK829_02640"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOU40143.1"
                     /translation="MVRVLFVCLGNICRSPMAEAVFQRMVEDAGLTDKVQVDSAGTSA
                     YHVGEPAHPGTQRILSHHGIQYRGRARQLAGSDVTGDQVYLIAMDSDNAAELRRRFGE
                     SAPIHRLLDFAGHTAARDVPDPYYSDNFDYVYRLVEDGCRGLLDAVRADAGL"
BASE COUNT         1841 a         3589 c         3356 g         1842 t
ORIGIN      
        1 caagggcggc acgttcgacg cggctgccca tcacgccctg gcgcggcgcg tcgccggcgg
       61 gagcatggtg ttgctgaaga acaatggcct gctgccgttg caagcgccgc agcgcatcgc
      121 cgtcatcggc cgggcggccc tggaagccca cttccagggc ggcggcagtt cccacatcaa
      181 tccaacgcag gtagatgtgc ctttcgagga gttgcgccgg ctggccggcg acgccgagtt
      241 ccgctatgcc gcgggctacc ccgacgacga ctcccgcgac caggcgctca tcgacgaggc
      301 cgtggccgcg gcccaggcga ccgacgtggc cctgctctac atcgccctgc cgacgttcaa
      361 ggagtcagaa ggctatgacc ggcccgacat gggtctgaca acgcagcagg tagccctcat
      421 tcaggcggtg acggcggcac agccccgcac cgtcgttatc ctcaacaacg gcgcgccggt
      481 ggccatgagc gagtggatcg acggcgcggc ggccgtgttg gaggcgtgga tgatgggcca
      541 ggccggcggt ggggccatcg ccgacatcct cttcggccgc gtcaacccct ccggcaagct
      601 ggccgagacc taccccttgc gcctggtgga cacacccgcc ttcctcaact tccctggcga
      661 gaacggcgac gtgcgctatg gcgagggtct gttcatcggc tatcgctatt acgacaccaa
      721 gcagatgccg gtgcagttcc cgtttggcta tggtctgagc tacaccacct tcgcctatga
      781 ccacgtgcgc gtctcggccg gcaacggtgt ccccaccgtt cacttccggg atgtggatgg
      841 cgtcactgtg tcggtggacg taaccaacac gggcgccgtg gccggcaagg aagttgtgca
      901 ggtctatgtc cacgaccgcg ccgcgcggct catccggccg gcgaaggagc tgaagggctt
      961 cgccaaagtg gcgctggagc cgggcgagac caggacggtc aatattccgc tcgacttccg
     1021 cgccttcgcc tactacgacc cggcctatca ccagtggatc accgaggatg gcgagttcga
     1081 catcctcgtg ggcgcgtcgt cggccgacat ccgcggccag gccacggtga cgctggaatg
     1141 caccctggag ctgcccagtc tgctgcacga cgaatcgacg atccgcgctt ggttcgccga
     1201 tcccattggc cagcctatcc tgcaacccat cttcgacgag ttgatgaaga agggcggcgt
     1261 gtttgccggc aatggctctg aagagccagt ggatatggat ctgttgagct tcctgatgga
     1321 tttgccgttg cggagctttt tccacttcca ggaaggctac ctgacccagc cgccagacga
     1381 cattaccaac atgctcctgg agcaagtaca cggcctgggc agccgcaagc tggccctggc
     1441 cccggcgcta tagagaagcg acccgcgcgg ttgggccagc cctgggcggg cgcagccggc
     1501 gggcggaggg acaaccagcg gcggcatggg gccggtgagg atgaccatcg accacacgcc
     1561 gccgcgcctt tttctctggg gccggtcgcc ggcgcgccgc cgactgaccg ccacatgagc
     1621 aagttcgccc caccctctca cctttaggat aatcgtaacc cagtactgga atagtagaga
     1681 gcgcctatga ccgaccaaca accgccccct cccccacgcc gccgatccgc gcggcgcgtt
     1741 gcccgcaagg ccacccccac aaacagccct ggcctgggtc tggccgcgct cgtcgtcctg
     1801 ctggccacgc tgccgctgct gggcattctc tatctgggcg cgcaattgct ccgtttgccc
     1861 ttcccgccgc tcgacctgta cgactggccc gtgcgcgccg gcttcacgcc gtgggccgcg
     1921 ctggtcgact ccctgagcag ccctacgggc aacatcgccg aaaccgcccc gcttgtccag
     1981 tggctcgtcg ggctggcgct gttcctggca ttggcctatc tggtgggtct ggccttctac
     2041 gcctttgtca tgcgccgcgg acgcgcccct gacgccattg atggcctgac ggctggcgcg
     2101 ctgctggctg tgccgatggt tttcgtcagt ctggccgtgc gcagttcgcc gctgcctgcg
     2161 ccgctcatca tcgtctggtt ggtggcagcg ctcgttggct gggggctgct gctcagctat
     2221 gcgttcggcc gcctgctgcg ccctgcgccg gccgtcgtgg ccccggccat gcttccgtcc
     2281 gaagagccag tcacgcccat cgccccggcg gcggcgacaa ccccaacgcc ggcgggcatt
     2341 gaccgccgcc gcttcctgct acaatttggg gccagcaccg cggccatcac cgccgtcacg
     2401 accgccgctg gcgcggctct ggcccgccgc gaggacgccg agacgttgca gcggacgctg
     2461 ccgatgatca gcccggagtt ccgcgcggcg cagcaggagt tatttggtcg cttccgccgc
     2521 ttcgccatcg tccgcggcgc agccgactcg gtggacaaga cgaacgttct ggccctgggc
     2581 gcggagtatc ccgaccgcaa ctacgtctcg atctggctgg gaggccacag ccccattgtc
     2641 atctacgaga atctggagac ggccctggcc gcctatagcg ccgaagggga acagaccggc
     2701 ctgttctggc tcgatagcta acattctttg tccccagccc agacgcaccg acccgacagg
     2761 tgggcagcca cctgtcaggt cattggccgc tagacgactt tggaaaagcc cactgggccg
     2821 gcggccttta gttgacagcg gccaacgtct aggcgtatca tcccataggc agccgggcga
     2881 cgcggcccac gcgcccgcgc ccttgcgccg ctgcaaatcc ccgcagagga agggaaccaa
     2941 atggtcaata gacagactcc ctcgcccatc agccccgatc tcctggagat tctgcgcgac
     3001 ccgcaggcta tccaggaagg cgaccgctac ggcgccgacc ccggccggct ggaactggtg
     3061 cgcggctgct ggctggtgtc ggctgacacc ggctacaagt accccattcg cgatggcatc
     3121 ccggtgatgc tcattgagga gggcgcgcgc tggaaggaca cgccggtggc agccttgcct
     3181 gtgccgccgc ccccggctat cgacgcgccc gccccagaga cgcgcgcgga cgaggtcgcg
     3241 accatgccgg cggccaacga gacaaatatc gccttgctcg tcgccatcgg cgcgctggtg
     3301 ctggctctgc tcattgtcat cggcctgagc cgccgtcatg cggcgtcgcc cgagccgctg
     3361 ccggaaccgc tcgtgccatg acgcgcctga cggacagccg tctggctaac gtcggcgcgg
     3421 gaactctgca gaacgcctac aacaccttcc acgaccagtt ccaggccatc accggccagg
     3481 ccaagcaccg cttcgagatg caggactggg gcggcctgca agaggacgcg gccgcgcgtc
     3541 tgggcctcta ccggcgcatc gtcgagctag ccgaggcggc catccgcgac ctgctccgcg
     3601 agcggctgga caacaagatc atctgggcca gcatgaaggc ggtctactcc agtctgatcg
     3661 cccagcgcga tgactgggaa gtggccgaga ccttttttaa ctcggtcacg cgccgcattt
     3721 tcaccactgt cggcgtcgat catcagatcg agtttgtggc caccgacttt gagacgccgc
     3781 cgacgcggcc gcagtcacct gtctaccgcg tctacagccg catcacgtcc acgacagatc
     3841 tggtgatggc catcctgcgc gacagccgct tcgcggcttc gtttcgcgac atggccgagg
     3901 acgcccatct agcagccgcg cgcatcgacg cgcagttgga ggcgctcaac acgctgcaca
     3961 ctgtccagcg ggtggagatg gtcaagcccg ctttctatcg cggcatgggg gcttatctgg
     4021 tcggccggct cttcagcggc tcacagagct tccccctggc gctggccctc attcacacgc
     4081 cagagggcat taccgtcgat agcatcctgc tagacgagga tagcgtcagc atcctgttca
     4141 gctttgccca ctcttatttt cacgtcgatg tcgcgcgccc ctacgacctg gtgcactttc
     4201 tcaaaactat cgtgccgcgc aagcgcacgg gcgagttgta tatttccatc ggctacaaca
     4261 agcatggcaa gacagagtta taccgcgacc tgctcgacca cctggcctat acccaggagc
     4321 gctttgagat cgctcgcggg cagcggggca tggtcatgct ggtgttcacc atgcctggct
     4381 acgatattgt cctgaagctg atcaaagacc acttcaacta tcctaaggat tcaacgcgca
     4441 aggaggtcat ggccaagtat gacctcgtct atcgccacga ccgtgccggc cgcctggtcg
     4501 atgcgcaatc gttcgaatat ctggagttca gccgcgacct gttttcgccg gaactgctgg
     4561 aggcattgca gcgcgattgc agccagtcag tcgtcatcgg ccagaagacg gtcgtactgg
     4621 cccacaccta ctttcagcgc cgcgttatcc cgctggacat cttcacccgc gaggcgcgtg
     4681 aggccgccgc ctgcgccgcc gtggtcgatt acggccaggc gatcaaggac atggcttgca
     4741 gcaatatctt ccccggcgac atgctgctga aaaacttcgg cgtcacgcgc cacggccgtg
     4801 tcgtcttcta cgactacgac gagttgtgtc tgctggagga gtgcaatttt cgccgcttgc
     4861 cgccgacaga tcgctatgaa gaggagctgg ccgccgagcc gtggttccac atcaacacca
     4921 acgacatctt cccggaggag ttccccaact tcctggggct cagcggttcg ctgcgcgcgg
     4981 tgttcgagag cgctcacagc gacctctaca ccgttgacta ctggcgcgcc cgccaggagg
     5041 ccatccgcgg cggggaacgc gcccatatct tcccgtatca ggcgggacaa cgtctacatc
     5101 aaggagtcta gagtatggag tatcgtcgtc tgggcaagtc cgggttgccg gtcagtgctc
     5161 tatcgtatgg ctcgtgggtc accttcagcc accagttagg cgtcgagcag gccctggaga
     5221 ccatgagcgc ggcctatgaa gccggcgtta actttttcga caacgccgag gtctatgccg
     5281 gcggccggtc ggagacgatc atgggccagg cattgcggca ggtcggctgg ccgcgcgaca
     5341 gctacatcgt ctccagcaag gtcatgtggg ggtcggtgga cgaccccaag ccaacccagc
     5401 gcggcctgca ccgcaagcac gtcttcgagg cctgccacga tgccctgcgc cgcttacagg
     5461 tcgattacct cgacctctac ttctgccacc gccctgacat caacgtgccc atggaagaaa
     5521 tcgtccgcat gatgaccgaa ctgatccacc agggcaaggt gctctactgg ggcacgtcgc
     5581 aatggtcggc gcagcagctg gccgaggcct acggcgttgc ccggcagtac cacctcattc
     5641 cgccgacgat ggaacagccg caatacagca tgctccaccg cgacaaggtc gagcgcgagt
     5701 tcctgccgct ctatgagacg atgggcctgg gcacgacgac gttctcgccg ctctactttg
     5761 gcatcctgac cggcaagtac aacgatggca tccccaccga ttcgcgcgct aacctggccg
     5821 gctacgagtg gatgcgcgac aacatcgaga gcgacgaggg ccaggccaac ctggagaagg
     5881 tgcgccggct gacgcccatc gccgaggggt tgggcacgac catgagccgg ctggccatcg
     5941 cctggtgtct gcgcaatccc cacgtcagca cggtcatcct gggcgcgtcg cgcgtggagc
     6001 aagttcacga caacctgcac gcgctcgaac tcctgccgca actgactgat gacgtaatgg
     6061 ccgccatcga cgaagtgctg gccaacaaac cggcggcgcc ggaaacctac taagcgtaag
     6121 cgccgcgaga agccatgagc caacgtttgc tacccatcac cggtctgccc gtcggtgagg
     6181 tcaccccggc ggtcatcgtc tgcggcgacc cggcgcgggc cgcgcgcatc gccgcccgcc
     6241 tggacgaggc cacaccgctg agccaccacc gggagtatcg cgccctgcgc ggcggctacg
     6301 agggcctgcc actgacggtc tgctcccacg gcgtcggcgc gccgggagcg gccgtggcct
     6361 tcgaggaact gatcgccgcc ggggcgcggc agatcgtgcg cgtgggcacg tgtggcgcgc
     6421 tgcaagccgg catggacgcc ggccacctgg tcgtggccac ggccgcggtg cagaacatcg
     6481 gctacggccg cgagactgtc cccgatggct acccggccgt ggccgacccg accctgacgc
     6541 tggccctggc tcgcgccgcg accgaggccg ggccgcccgc ccacagcggc ttcgtcatct
     6601 cgcgcgacag cttttacccc ggcgtgcccc tggccggcgc gcccgattac cgcgttctct
     6661 ccgccgcccg cgtgctggcc gtggagatgg agtgcgcggc gcttttcctc gtcggcagcc
     6721 tgcgcggcgt ggccacggcg gccatcctgg ccgtggacgg caacgtgctg gccagcgccg
     6781 agagcatgga cagctatcag cccgggcagg aagttgtcgg tgtcgccgtg gaggcggcca
     6841 tcggctgcgc cttgcgggcg ctacggagcg tggcatgacc ggcatccagg acaagcggcg
     6901 ggccatccgc cacctgttgc gcgagcgcga gccggctgac gcgatggccg cctactacgc
     6961 gctctatcac cccgacgagc ggacgacgct cattgtgtcg ccgctggtag acggtcagca
     7021 cgccgatggc tatgtggccc tttcgcggac ggggatcgac ctgttccggc cgttggtaac
     7081 catgcgcctg cccaccgacc gcgacccgac ggcgaccgag gccgccgccg ccctcttgcg
     7141 ccaggcgctt ggccccggcc aaccggtcat catcagcgcc ccggaccgct atctgcccct
     7201 gctgcgggcg ctgggcgaca cccagtcgga ggaacgcctg cgcctgtatg ctctggacgg
     7261 ccggcgattc gagccggtca tcaatgtgct ggtgacgcgc ggcgaggggc cgggcgggct
     7321 gccgcgcttc accatccgct cgtcgcagac gggcacggaa gaggtggcgg ccacggcgtc
     7381 gctcaactgg cagtcgccca ccttcgccga gatcgccgtc agcacgcgcc cacagttccg
     7441 gcgacagggc tgggggcgca gtgtggtcgc cgctctgtgc caatacgtgc tggagagcgg
     7501 tcgcacaccc ctctacgtcg tcgctgagga gaacgcggcc tcgatccagc tggccgaaag
     7561 cctcggcttt gccgacactg gcgcgcgcga agcctttttg cagaccatgt tgcgccctat
     7621 ctcatagggt cgctgctccg ctaatcgcgc cgcgccgcgc caacctgggg ccagtgcgcg
     7681 ccccaattgg cccctgcgcc gccgccccaa aaaggcgtgc agacgccgtg aagaaggcgt
     7741 gaaatgtcgt ctgcccccgt tgaccctctc cggcccaata tgctaatctg ccagtaaccg
     7801 caaatgtatt ggtagcctcg gtacacgcca ggtattggta gtccgctaca gttgaagcac
     7861 cattttacgg aagcgaaatg gtgctttatc tgaggccctc cgtagtcaga gcgctttgga
     7921 gcaatccgta gtaacgttgg aggtattggt tctatgttcc gcgcttcagg tattgtgaag
     7981 tggttcagcc gcttgaaggg gtatggcttc atcaacccgg acgaaggtgg acaagaggtc
     8041 ttcgtccact attcagccat cgacggcgat ggctatcgca atctctacga aggcgaccgg
     8101 gtggagtatg atctgatcga tcagggcaaa ggcccccaag ccaagcgcgt cgtccccaag
     8161 cgctaggcga ctagcgcccc cgttgtcatc gttcacgagc gcccgccctg cgcgggcgct
     8221 tcctttttct ggtcaataca cgctacgacg atacaatcag cgattacctt atctttgggc
     8281 gggaacgcgg gtttctatga gccattcggt cttaatcgtt gacgacaacc tttcgttggc
     8341 gaacctttat cagattgtct ttgaacgggt gggctgggcg gttacgcggg tgtccagtgg
     8401 ccaggaggcg ctagacagca tcgccggccg ggtgccggat gtgatcctgc tggatgtgat
     8461 gatgccggtc atggatggca tcgaagcgtg ccgccgcatc cgcgccctgc gaccagagaa
     8521 gccaccctgc atcgtcatct actcagccaa caaccggccg gaagtgcgcg agaccgttct
     8581 ggccgccggg gccgatgcct ttgtcagcaa gaacctctct gtcttcgagc tgccctcgca
     8641 catcctcgtc tatctgccgg aaggctcacg ctagagctta gaaacgaaga ccttggccag
     8701 gccgccgtgc cggcccagga agcgaatcag ccggtcgacc agcataggat agttcatcag
     8761 gaagcgctgg tagaagaagg acgtgcgcat ccccgaccac atggtctcgc ggcagcgctc
     8821 ctcgtagacc tgcaagccgg cgcgactgac atccccgcgc cgcagggcct cgtcggccgt
     8881 ctcggccgcc agcaaggcgg aaatgagcgc gttatgtatg ccgccgccgg tcagcgggtt
     8941 gatgaacccg gccgcgtcgc ccaccagcag cgcgccgtcg aaggcgtact gtagcccctt
     9001 ctgcgagccg aagctcagtt gccaaatggc catgtcacgg acagtatgtc cttccctcag
     9061 ccgcgggcga atggccggca tgtccagaaa gtcatgcagc atcttcttga gattgtgcct
     9121 attgcggcgg aagacgtcga ggcgcatgcc caaaccgacg ttggccaggc cgtcgccagc
     9181 cgagaaaatc caggcgtagc cgggcagcac ttcttcatag agaaagaact cgaccttgtg
     9241 ggggaatatc tccataccct cgatgtaggc gcgtaaggcc acggcgcggt gcatatcgac
     9301 gtgctgctcg gtctccggcc gcaactggcg catgatggtt gaggtaacgc cgtcggcggc
     9361 gatgaccaac ggcgctcggt actcgtggac agcgccattg cgccgcgccc gcacgccaac
     9421 gacccggcca tcgaccagca ggggcgcttc gacctcagct tgctcgaagc acgctccaga
     9481 atcgatggca tgctgctgga tgacggcgtc gtagtagaga cgtggcgcga cataggaatc
     9541 ctcgcccacc gagccggggt gaaagtccat gtggagcgtg tgccccgagg gcgcgacgag
     9601 gcgcatgccg tccagcgggt agaactcgcc acgcgcgcgg gccgcggcga tcttatcggc
     9661 catgcccaag tcattcatga tgcggatggc ccccagcgag acagcgtcgc cgcagatctt
     9721 atcgcgcggg aagcgctgcc ggtcgaggag ggccacgtcg cgccccgcct gggccagggc
     9781 tgtggccgcg gtggcaccgc ctggccccgc gccaactaca atgacctcat gctcactcac
     9841 actcgccatt ctcgctgtct ctcctgctat gggttagacc atgtgaacag agcgcggccg
     9901 ggacaagtag ggaggggatg gaagccgaag tgggatcggc atcacggcgt tgcccggccg
     9961 cggtcaccgg ttacagaccg gcgtcggccc ggacggcatc gagaaggccg cgacagccgt
    10021 cctcgaccag ccggtaaaca tagtcaaagt tgtcactgta atagggatcg ggcacgtcgc
    10081 gggcggcggt gtggccggca aaatcaagca ggcgatggat gggagccgac tcgccgaagc
    10141 gccggcgtag ctcggcggcg ttgtcgctgt ccatggcgat caggtagacc tggtcgccag
    10201 tgacgtcgct gccggcaagc tgacgggcgc ggccacgata ctggatgccg tggtgcgaca
    10261 agatgcgctg cgtgccgggg tgcgccggct caccaacgtg ataggcgctt gtgccggccg
    10321 agtccacctg aaccttatcg gtcagcccgg cgtcctcgac catgcgctga aaaacagcct
    10381 cggccattgg cgagcggcat atattgccca gacagacgaa caggacacgc accatcacat
    10441 agtctcccga caggccggac aggcgtcgct gggcctgtca gatcacatcc gaacccgata
    10501 gcaagctggc ctataagccg cattctgtag gcgttggaag gccagcgccc ggtgatcatc
    10561 tctctggggc cgccgttacc ggcgacctcg tgcagcctac ccggacgtca gacgggacga
    10621 gcagcccc
//
LOCUS       DAOSMQ010000044        10415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6401_length_10415_cov_5.772780, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000044 DAOSMQ010000000
VERSION     DAOSMQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6401_length_10415_cov_5.772780"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK829_02645"
     CDS             <1..403
                     /locus_tag="PK829_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40144.1"
                     /translation="VHPTDDPLAGQLVEGCRHPLVVARLGPARLFRQPRGPFGIDGFL
                     RIAAREPGVYGNQIAVSARASGPAMYDFAFIFEGVPFENARQTVLGKPLAILTAETLA
                     PGPAGVLQAKAAGVHVTVSRERTAQGQALSS"
     gene            442..1434
                     /locus_tag="PK829_02650"
     CDS             442..1434
                     /locus_tag="PK829_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40145.1"
                     /translation="MANIEEPYVEFMAGDLIAAETMNEMQKMIRTNIGEQSQAAIEAI
                     ERVPRADNADALQGQNVEALTRDIVQKAISEMSRESGYRRVFKKLHLGDEPSVIQHDL
                     RNCPLVDLYQLEYFQVVASEDGYNRLTWVNFFIYHSSEKRIRYAPEGKKALNVEIESS
                     REPVFRIPLRDLLAWYAVDYDDDSSLGDIETEFWDKLFSDPNDRFDDDQYAHSPWFDR
                     CCRDERTVRSLKQKDDWNEIWIKIMPRKTVNYLTREQIAFTPHGSKPQPAPTQVEVAH
                     LDLNRIALQLLDKPIYSDEVIGVKAGDNEPGSGLLGEDREDALKELKLMVLLRA"
     gene            1572..6575
                     /locus_tag="PK829_02655"
     CDS             1572..6575
                     /locus_tag="PK829_02655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU40146.1"
                     /translation="MNLNASFPGETIRFEGHRTVVLRQLPAGAQIQSARVTVTPKAPP
                     GAPDRLFEEVLIFPAGGPLGDFGMTRVVAPGGGPVLGTVEVDFHARRTLAAVQGTNTT
                     GSGSIAGAVLLVDLGGGVFMPIAQNGALATPDNTDPFNVPAGGALPGLTVAKFRLQAA
                     AAAPPEQVQVLNVSQVTIRTVPTNVSVALEGLAPFWTRLGEMTAAETSDDFAQFLQLY
                     LNDAEVVGGFYEIPLVVHSDTLARLDLAVAIEYVVQQSALPAGVGEVALPYGHDTVPQ
                     DGCAALQVALPPGAQVTSAAVRVLGSFESSRVAVGPTGPVAPTAMAAVRPDQGQAQPI
                     RLARVVPATAIDLLLSCVSQAAVLEVNVLGDIDGKPFGDALLPRPVRIELARDIAPAP
                     TWLSAELPEAFQFPAGRVWLAVQAREGQAVWHAVAAAAAVAEADPIGLHYSAASGLTW
                     RASRGERAGAAPVNGAGDEALAGLFRLRHVPPEYRVPLEVLVGQGDGATRVSLDRYRA
                     LGRIDFTIDFPEFVAAINRAARAGEGSGAPQGEQLRNGDFARWTVAGDRIGAPQRVPG
                     IDIATTVSIPAHGQWAYVGEIPRTRQAEFAARFLDLPEHTGDMDPALPMPMRRQEVAI
                     RSDGERIAVMMASEANETRELFLVDGRARRVLASSDLDLALFCLAWSPDQHFLYSGAL
                     SGEAGEVQVIEAEALEQLMIRGEVGAIRTLLSHNSFTLSDGFLPISLALSADGRRLFV
                     AALQQGNTAAASSSRIYGFDTRTHSPLRGSPLDVPGRVVDLALTPDGGTLLAVNVTRQ
                     ALQAVDATRLTLARTIPLAASPNDILQPMVVVASGDGRRAYVGNAAGHSISVVDLTTW
                     QARRPIVIPTADDAGAAQFGSDVSLIDLALTPTGDRLYAAVLAVDKANQERGVAGLFY
                     LTLGLPEEWTLTQGYVMPLTLAEPHRLAALFGPLTAEECAAQPPRPSSLSQAVAASGG
                     RVYEFSFWGIALSAGATAEVIWRGDACALQHTDRVPVQIDEDAAIRPCPAIDPAAPDT
                     WPELLLHRVRLEAPAGATQAEIRFISPPGKNMLIDTVSFLATAETTVNGDLLQVDENG
                     EVVGWQLAPATAVGISLSPAPDGLRVGNTGSATASLLQSFAATAAQPFLLRFEGQIAA
                     VGAGGPPRVELRWLDAAGAPTGEVAALALQPDQTVHQLQGVAPTNAAGAELHLVLPGG
                     AALVVRHVSYAPVEMATVPICFVAEAPGDIAVLGFDYAYDTPAARPAPAPPAGLCAPT
                     PPGRRPDGKSGCMCAYCATPCGPCCDEEDGDDHAAAPAAAGLRSARPRRATQPARATR
                     PLIRAQGEAPESLVVPRLLPVEDDLRGFLALPAPAWAERAPELAAAAAPLTLVNGIGP
                     GRAAVLRQLGLGALPALAAADPQAIAVALNGVSPAMASGFVTEARTLLANPVGLPAPL
                     VSCIMPTANHRDRVPQAIALFRRQTYPHRELIIIDDGEDSVADLIPDEATLRAEGAAI
                     RYVRLDGRRTLGQKRNFGGEAAQGPIIAHWDDTTWYAPWRLSYQLGALVQREADGCGL
                     DSLLQYDPAAGTAWLSGPEPALFGGTLCYRRALWLARPFEESDDSDAAFLRDRPDARL
                     IALPAIDWLVGILLLSEESRPAARARRPYPAREVRRLLGDDAAIYERLAGRAPAPVG"
     gene            6632..7405
                     /locus_tag="PK829_02660"
     CDS             6632..7405
                     /locus_tag="PK829_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40147.1"
                     /translation="MAILQPVALLALSLLALVAIWALAQRVRLARARRRPAATIQLAD
                     AAIDYVEQLDERGELVVPDGASKSATKLALAVAWTRAELARLGIRLDDDEGTAWLRAR
                     LHQRRAELRPVAVMTAAAREAVDRVERLLMSDSTARLDHSERTLFYARLAADWFVVRL
                     AEQGATVGREEGQVWVQAELLRRLNARADDMAGRNDLDSLVLEANIFLDTLRANNRLP
                     AGSDAARDLAIAWVLTEVAKRGLAYTPREIVAAVRRVVR"
     gene            7729..>7954
                     /locus_tag="PK829_02665"
     CDS             7729..>7954
                     /locus_tag="PK829_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40148.1"
                     /translation="MPPSATSIGGARPRIYTIRPVRGPAESEARVAVQRAAFESEWMT
                     VERYARVRASPTYRPELDLVIEAPGGDLAGF"
     assembly_gap    7955..8054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8055..8372
                     /locus_tag="PK829_02670"
     CDS             <8055..8372
                     /locus_tag="PK829_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40149.1"
                     /translation="RPELDLVIEAPGGDLAGFALLWLDAANRLGVFEPLGVAAAYRRR
                     GLGRALMLAGLRCLGDCGADHALVETGLESRARSLYEATGFTLLDTNFAWVSPNSQEA
                     AET"
     gene            8369..10204
                     /locus_tag="PK829_02675"
     CDS             8369..10204
                     /locus_tag="PK829_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40150.1"
                     /translation="MSADLDRWLSPQTREAIEQRYYAEINRRARLETLIHDPSFLDAL
                     RTGEVHVGLFSDHGVVHVRDVARQIVTILERAHGLLIPRRPAWRFASMVGYGVLLAYL
                     HDIGMIDFSAFGRAMHPEYAAQAILSAEMDDLMATLWHENSGNLAWRLTQLAQAGALA
                     SEPRTVLRELLAMTIGHSKSKIPIDLLNDRPALRHRLLAVAGADLRVLYQQQRAERAR
                     QKLYAARQGGDAVEAERWSATLAESEREELAARIRPERHRPQLGDDAQGDNAQGDNAF
                     AWLMSAHPDVRALADDAIDTVRALRAADSLRQRGLVLKTSGGYEMFISRVSGRAVYSL
                     QANDGRLYLLEVSDSLSSGEAILAGSELDGNGDLRIAFHRGAFDTPAATDYAVAVTAT
                     VVDDIIQDTIISFQRAPDEPAEGLKPATAMRILLEETDDSAGFVARVRQRLIADEPSL
                     SERVRLAPSLREVSERERALYLSGTSIAWDRPTRQQFLDRLTLSGQRCEGIDVDRAFA
                     DVRLVRLRPGEVVIDAGAPAAFVYIPLGEGLRIVPLGGYDTIAARAWSLLGTTGVIRG
                     AARNATVIADVALEMVMIPKGVYMREWYRTHSPDDFLLLLGNMDA"
BASE COUNT         1545 a         3639 c         3555 g         1576 t
ORIGIN      
        1 agttcatccg accgatgatc ccctggccgg ccagctcgtc gaaggctgcc gccacccgct
       61 ggtagtcgcc cggctcggtc cagcccgcct gttccgccag ccgcgcggcc cattcggcat
      121 cgacggcttc ctgcgcattg ccgcgcgcga gccgggggtc tatggcaacc agatcgccgt
      181 ctcggcccgc gcgtctggcc cggccatgta cgacttcgcc ttcatcttcg agggcgtgcc
      241 ctttgagaac gcgcgccaga cggtgttggg caagccgttg gccatcctga cggccgagac
      301 gctggccccc ggcccggccg gcgtgttgca ggccaaggcc gccggcgtcc acgtcaccgt
      361 cagccgtgag cgtacggcgc aggggcaggc gctgagcagt taatgtgatc gaactgatac
      421 atgcagaaag gagatagtga aatggcaaat atcgaagagc cgtatgtgga gttcatggcc
      481 ggcgacctca tcgccgcgga gacgatgaac gagatgcaga agatgatccg gaccaacatc
      541 ggcgagcagt cgcaggcagc catcgaggcc atcgagcgcg tgccgcgcgc cgacaacgcc
      601 gacgccctgc aggggcagaa cgtcgaggcg ctgacgcggg acatcgtcca gaaggccatc
      661 agcgagatgt cgcgcgagag cggctaccgg cgcgtgttca agaagctgca cctcggtgac
      721 gagccgtcgg tcatccagca cgacctgcgg aactgcccgc tggttgacct ctaccagttg
      781 gagtacttcc aggtcgtggc ctcggaggac ggctacaacc gcctgacgtg ggtcaacttc
      841 ttcatctacc actccagcga gaagcgcatc cgctacgcgc cggagggcaa gaaggctctg
      901 aatgtcgaga tcgagtcgtc gcgagagccg gtcttccgca tccccctgcg cgacctgctg
      961 gcctggtacg cggtggacta cgacgacgac tccagcctgg gcgacatcga gacggagttc
     1021 tgggacaagc tcttcagcga ccccaacgac cgcttcgacg acgaccagta cgcccactcg
     1081 ccctggttcg accgctgctg ccgcgacgag cggacggtgc gcagtctgaa gcagaaggac
     1141 gactggaatg agatctggat caagatcatg ccgcgcaaga cggtcaacta tctgacgcga
     1201 gagcagatcg ccttcacccc ccacggctct aagccgcaac ccgcacccac gcaggtagaa
     1261 gtcgcgcacc tcgatctgaa tcgcattgcc ctacaactgc tcgacaagcc aatctactcc
     1321 gatgaggtta tcggtgtcaa ggcgggcgac aatgagcccg gaagcggtct gctgggcgaa
     1381 gaccgcgaag acgcgctgaa ggagttgaag ctgatggtct tgctgcgcgc ctgaccgtcc
     1441 gcggaggccc tcctggcccg tcttctcccc gccgggaacg gctccgggag aggagggcct
     1501 ccgactgaac tctggtttga aggtggacaa caggcgctcg gctgacgaga catagggcaa
     1561 ggcgttgaca catgaatctg aacgcatcat ttccaggcga aacgatccgc tttgagggcc
     1621 atcgcaccgt tgtgctgcgc caactgccgg ccggggcgca aatccagagc gcgcgcgtca
     1681 ccgtcacgcc caaggccccg ccgggcgcgc ccgaccgcct cttcgaggaa gtgctgatct
     1741 tccccgctgg cggccctctg ggcgactttg gcatgactag agtcgttgcg cccggtggag
     1801 ggccggttct gggcacggtc gaggtggact tccacgcgcg gcgcacgctg gcggcggtgc
     1861 agggcacaaa caccaccggc agcggctcca ttgcgggggc cgtgctgttg gtcgatttgg
     1921 gcggcggcgt cttcatgccc atcgcccaga acggcgcgct ggccaccccg gacaacaccg
     1981 atcctttcaa cgtgcccgcc ggcggcgcgc tgccggggct gacggtggcc aagttccgcc
     2041 tgcaagcggc cgcggccgcg ccgcccgagc aggtgcaggt tctcaacgtc agccaggtga
     2101 ctatccgcac cgtcccgacc aacgtcagcg tcgccctgga ggggctggcc cccttctgga
     2161 cgcgcctggg cgagatgacc gcggccgaga cgagcgacga cttcgcccaa ttcctacaac
     2221 tgtacctgaa cgacgccgag gtggtgggcg gcttctacga gatcccgctc gtcgtgcaca
     2281 gcgacacgct ggcccggcta gacctggccg tggccatcga gtacgtcgtc cagcaatcgg
     2341 cgctgccggc cggcgtgggc gaggtggcgt taccttatgg ccacgacacc gtgccgcagg
     2401 acggctgcgc ggcgctgcaa gtggccctgc cgccgggggc gcaggtcacg agcgccgccg
     2461 tgcgcgtcct cggctcgttt gagtccagcc gcgtcgccgt cggcccgacg ggaccggtcg
     2521 cgcccacagc tatggcggcg gtccggcccg accaggggca ggcgcagccc atccgcctgg
     2581 cgcgtgtcgt gccggcgacg gccatcgacc tgctgctgag ttgcgtcagc caggcggccg
     2641 tgctggaggt gaacgtgctg ggcgacatag atggcaagcc gtttggcgac gcgctgctgc
     2701 cgcggccggt gcgcatcgag ctggcccgcg acatcgcccc cgcgcccacc tggctcagcg
     2761 cggagttgcc ggaggcgttc cagttcccgg ccggccgcgt ctggctggcc gtgcaggcgc
     2821 gcgagggcca ggccgtctgg catgccgtcg ccgccgcggc ggctgtggcc gaagccgacc
     2881 ccattggcct gcactactcg gccgcgtctg gcctgacctg gcgggccagc cgaggcgagc
     2941 gcgccggggc cgcgccggtc aatggcgcgg gcgatgaggc gctggccggg ctgttccggc
     3001 tgcgccacgt gccgcccgag taccgcgtgc cgctggaagt gctggtcggc cagggcgacg
     3061 gggccacgcg cgtgtcgctc gaccgctacc gggcgctagg gcgcatcgac ttcaccatcg
     3121 acttccccga attcgtcgcc gccatcaacc gcgccgcgcg ggcgggcgaa ggcagcggcg
     3181 cgccccaggg cgagcagctg cgcaacggcg acttcgcccg ctggaccgtg gccggcgacc
     3241 gcatcggcgc gccgcagcgc gtgccgggca tcgatatcgc cacgacggtc agcattcccg
     3301 cgcatgggca gtgggcttac gtcggcgaaa taccgcgcac gaggcaagca gagttcgccg
     3361 cgcggtttct cgatcttccg gagcacaccg gggacatgga tccggcgttg ccgatgccca
     3421 tgcgccggca ggaggtggcc atccgcagcg atggcgagcg gattgccgtc atgatggcca
     3481 gcgaggccaa cgaaacgcgc gagctgtttc tcgtcgacgg ccgcgcgcgg cgagttctgg
     3541 ccagcagtga cctggacctg gcccttttct gcctggcctg gtcgcccgat cagcactttc
     3601 tctacagtgg cgcgctcagc ggcgaggccg gcgaggtgca ggtcatcgag gctgaagccc
     3661 tggaacaact gatgatccgt ggggaggttg gggcgatccg gacgctgttg agtcacaatt
     3721 cgtttacgct gagcgacggc tttttaccca tcagcttggc gctctcggcc gacgggcggc
     3781 ggctctttgt cgccgcgctc cagcagggca acaccgccgc cgcgtccagc agccgcatct
     3841 atggcttcga cacgcgcacg catagcccat tgcgcggctc gccgctggac gtgcccggca
     3901 gagtggtcga tctggccctg acgcccgacg gcggcacgct tctggccgtc aacgtcaccc
     3961 gccaggcgct gcaagccgtg gatgccactc gtctgacgct ggcgcgcacc atcccgctgg
     4021 ccgcgtcgcc caacgacatc ttgcaaccga tggtcgtggt ggcgtctggc gacggccgcc
     4081 gcgcctacgt cggcaacgcc gccggccact cgatcagcgt cgtcgatctg accacttggc
     4141 aagcccgccg gcccatcgtc atccccacgg ccgacgacgc cggcgccgcg cagttcggca
     4201 gcgatgtctc cttgatcgat cttgccctga cccccacggg cgaccgcctg tatgcggcgg
     4261 tgttggccgt ggataaggcg aatcaggagc gcggcgtggc cggactcttt tacctgaccc
     4321 tcggcctgcc ggaagagtgg acgctgacgc agggatatgt catgcccctg acgctggcgg
     4381 aaccccaccg tctggcggcg ctgttcggcc cgctgacggc cgaggagtgc gccgcgcaac
     4441 cgccgcggcc gtcgtcgctg tcgcaggccg tggccgccag cggcgggcgc gtctacgagt
     4501 tcagcttctg gggcatcgcc ctgagcgccg gggcgacggc cgaggtcatc tggcgcggcg
     4561 acgcctgcgc cctgcagcac accgaccgcg tgccggtgca gatcgacgag gacgcggcca
     4621 tccggccctg cccggccatc gacccggccg cgcccgacac ctggccggag ctgctgctgc
     4681 accgcgtgcg gctggaggcc cccgccggcg cgacccaggc cgagattcgc ttcatcagcc
     4741 cgccgggcaa gaacatgctc atcgacacgg tgtcgttcct ggccacggcc gagacgaccg
     4801 tcaacggcga cctgttgcag gtggacgaga acggcgaggt cgttggctgg cagttagcgc
     4861 cggcgacggc cgtgggcatc agcctgtcac cggcacccga tggcctgcgg gtgggcaaca
     4921 cgggcagcgc cacggccagc ctgttgcaga gcttcgccgc caccgccgcc caacccttcc
     4981 tcctgcgctt cgagggacaa atcgccgccg tgggcgcggg cgggccgccg cgcgtggagt
     5041 tgcgctggct cgacgccgcc ggcgcgccca cgggcgaggt cgccgcgctg gcgctccagc
     5101 ccgaccagac cgtgcaccag ctccagggcg tcgccccgac caacgcggcc ggcgcggaac
     5161 tgcatctggt gctgccgggc ggcgcggcgc tggtggtgcg gcacgtatcc tatgcgccgg
     5221 tggaaatggc cacggtgccg atctgctttg tggccgaagc gcccggcgac atcgccgtgc
     5281 tgggcttcga ttacgcctac gacacgccgg cggcgcggcc cgcgcccgca ccgccggccg
     5341 gcctgtgcgc gccgacgccg cccggccgcc gcccggacgg caagtcaggc tgcatgtgtg
     5401 cctactgcgc cacgccctgc ggcccatgct gcgacgaaga ggacggcgac gaccacgccg
     5461 ccgcgccggc agccgccggc cttcgtagcg cccggccgcg ccgggcaacg cagcccgccc
     5521 gcgcgacccg cccgctcata cgcgcccagg gcgaagcgcc ggaaagcctg gtcgtgccgc
     5581 gcctcctgcc tgtagaggac gacctgcgcg gcttcctggc tttgcccgcg ccggcgtggg
     5641 ccgagcgcgc gcccgaactg gccgccgccg ccgcgccgct gacgctcgtc aacggcatcg
     5701 gccctgggcg cgccgcggtc ttgcgccagc tgggactggg cgcgctgcca gcgctggccg
     5761 cggccgaccc gcaggccatc gcggtggccc tcaacggcgt ctccccggca atggccagtg
     5821 gtttcgtcac cgaggcgcgg acgctgctgg ccaacccggt cggactgccc gcgccgctgg
     5881 tgtcgtgcat tatgcccacg gccaaccatc gcgaccgcgt gccccaggcg attgccctgt
     5941 tccggcggca gacctatccc catcgtgagt tgatcatcat tgacgacggc gaggactcgg
     6001 ttgccgatct catcccggac gaggccaccc tgcgcgcgga gggcgcggcg atccgctacg
     6061 tgcgcctcga cggccggcgc acgctcgggc agaagcgcaa cttcggcggc gaggcggcgc
     6121 aggggccgat catcgcccac tgggacgaca cgacgtggta cgccccctgg cggctaagct
     6181 accagttggg cgcgctggtg cagcgcgagg ccgatggctg tggcctggac agcctgctcc
     6241 agtacgatcc cgccgccggc acggcctggc tgtccggccc ggagccggcg ttgttcggcg
     6301 gcacgctctg ctatcgccgc gctctgtggc tggcgcggcc gttcgaggaa agcgacgaca
     6361 gcgacgccgc cttcctgcgc gaccggcccg acgcccgcct tatcgccctg ccagctatcg
     6421 actggctggt aggcatcctg ctactcagcg aagagagccg cccggccgcg cgcgcgcggc
     6481 ggccctatcc cgcgcgcgag gtgcgccggc tgctgggcga cgacgcggcg atctacgagc
     6541 ggctggccgg gcgcgcgccc gcgccggtcg gctaaggggc gctgctggcg tctatgtgga
     6601 ggtcactacg gaaccgatac tgaccacggc gatggccatc ctgcaaccgg ttgccctgct
     6661 ggcgctgagc ctgctggcgc tcgtcgccat ctgggcgctg gcgcagcgcg tccgcctggc
     6721 gcgcgcgcgg cggcggccgg cggcgacgat acaactggcc gacgccgcca tcgactacgt
     6781 tgagcagctc gacgagcgcg gcgagctggt cgtgcccgat ggcgcgtcga agagcgcgac
     6841 caagctggcc ctggccgtcg cctggacgcg ggccgaactg gcgcggctgg gcatccgtct
     6901 ggatgacgac gagggcacgg cctggctgcg ggcgcggctg caccagcgcc gcgccgaact
     6961 gaggccggtg gcggtcatga ccgcggcggc gcgcgaggcg gtcgatcgcg ttgaacgttt
     7021 gctgatgagc gactcaaccg cccggctaga ccacagcgag cgcactctgt tctatgcccg
     7081 gctggcggcc gactggtttg tcgtgcggct ggccgagcag ggggcgaccg tcggccggga
     7141 ggaagggcaa gtctgggtgc aggccgaact gctgcgccgc ctcaatgccc gcgccgacga
     7201 catggccggc cgcaacgacc tcgattcgct ggtgctggaa gccaacatct tcctggacac
     7261 gctgcgggcc aacaatcgtc tgccggccgg cagcgacgcc gcccgcgacc tggccatcgc
     7321 ctgggtgctg accgaggtgg ccaagcgcgg cctggcctac acgccgcggg agatcgtcgc
     7381 cgctgtgcgg cgggtcgtgc gctaggacag atttgacgtt gagcgggcgg cggagttcac
     7441 gccaaggcgc aaagagggca aaggaggcca agtagaagct cgatcattgg ccgttccctc
     7501 tttgcgcctg ttgccccttt gcgtgagcgc ctatcgactt ggcaatgacc gggtaggcta
     7561 ttccctaccg cggcggcggc gcttatgcta ctctatgagc agtcatgccc agagaggatt
     7621 gacttgacac catgcgcatc catccccgcc cctttcagtc tgagtccgac tacggggcca
     7681 tgcgccgcct tgtggtcgat gtcctggccc acaccggccc acccgtctat gccaccatcg
     7741 gcaacctcga ttggtggcgc gcggccgagg atctacacca tccgtccggt gcgcggcccg
     7801 gcggagagcg aggcgcgggt cgccgtccag cgcgccgcct tcgagtcgga gtggatgacc
     7861 gttgagcgct atgcccgcgt gcgcgcgtcg cccacctacc ggccggaact cgatctcgtc
     7921 atcgaggccc ccggcggcga tttggccggt tttgnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnncggccg gaactcgatc tcgtcatcga ggcccccggc ggcgatttgg
     8101 ccggttttgc cctgttgtgg ctcgacgcgg ccaaccggct gggcgtcttt gaaccattgg
     8161 gcgtggccgc ggcctaccgg cggcgcgggc tgggacgggc gctgatgctg gccgggctgc
     8221 gctgcctggg cgactgcggc gctgatcacg ccctcgtcga gaccggactg gaatccagag
     8281 cgcgtagcct ctacgaggcc actggcttca cgctgctgga caccaatttc gcctgggttt
     8341 cgcccaacag ccaggaggca gctgagacat gagcgccgac cttgatcgct ggctctcgcc
     8401 ccagacgcgg gaagccatcg agcaacgcta ctacgccgaa atcaacaggc gcgcccgcct
     8461 ggagacgctt atccacgacc ccagcttcct ggacgcgctg cgcactggcg aagtacacgt
     8521 gggccttttc tccgaccacg gcgtcgtcca cgtgcgcgac gtggcccggc agatcgtcac
     8581 catcctcgaa cgagcgcacg ggctgctgat tccccggcgg ccggcgtggc gcttcgcgtc
     8641 gatggtgggc tatggcgtcc tgctggccta cctgcatgac atcggcatga tcgacttctc
     8701 cgccttcggc cgggccatgc accccgaata cgccgcgcag gccatcctga gcgccgagat
     8761 ggacgacctg atggccaccc tgtggcacga gaacagcggc aacctggcct ggcggttgac
     8821 gcaactggcg caggcgggcg cgctggccag tgagccgcgg acggtgctgc gcgagctgct
     8881 ggccatgacc atcggccaca gcaagagcaa gattcccatc gacctactca acgaccggcc
     8941 cgctctgcgc catcgtctgc tggccgtcgc cggcgccgac ctgcgcgtcc tataccagca
     9001 gcaacgcgct gagcgggcgc ggcagaaact ttacgcggcg cggcaggggg gcgacgcggt
     9061 cgaggctgag cgctggagcg cgaccctggc cgagtccgag cgcgaggagt tggccgcccg
     9121 catcaggccg gaacgccacc ggccccagtt gggcgacgac gctcagggcg acaacgctca
     9181 gggcgacaac gcctttgcct ggctgatgtc ggcgcatccg gacgtgcgcg cgctggccga
     9241 tgacgccatt gataccgtcc gcgccctgcg cgcggccgac tcgctgcgcc agcgcggtct
     9301 ggtgctgaag acctccggcg gctatgagat gttcatcagt cgcgtgagcg gccgggccgt
     9361 ctactcgctg caggccaacg acgggcgact ctatctgctc gaagtatccg actccctcag
     9421 ctccggcgag gccattctgg ccggcagcga gctggacggc aatggcgatt tgcgcattgc
     9481 cttccaccgc ggcgcgttcg acacgcccgc ggcgacagac tatgccgtcg ccgtcacggc
     9541 caccgttgtt gatgacatca tccaggacac catcatcagc ttccaacgcg cgccggacga
     9601 gccggccgaa gggctgaagc cggcgaccgc gatgcgtatc ctgctggagg agacggacga
     9661 ctcggccggt ttcgtcgccc gcgtccgcca gcgccttatc gccgacgagc catcgctgag
     9721 cgaacgggtg cgcctggccc catcgttgcg cgaggtgtcg gagcgggagc gcgcgctcta
     9781 cctgtccggc acgtccatcg cctgggatcg gcccacccgg caacaattcc tggatcgtct
     9841 gacgctcagc ggccagcgct gcgagggcat cgacgtagac cgggccttcg ccgacgtgcg
     9901 cctggtgcgg ttgcggccgg gcgaagtcgt cattgatgcc ggcgcgccgg ccgcgttcgt
     9961 ctatatcccc ctgggcgagg ggctgcgcat tgtcccactg ggcggctacg acaccatcgc
    10021 cgcccgcgcc tggtcgctcc tgggcaccac cggcgtcatt cgcggcgcgg cccgcaacgc
    10081 caccgtcatc gccgatgtgg cgcttgagat ggtcatgatc cccaagggcg tctacatgcg
    10141 cgagtggtat cgcacgcact cgccggatga ctttctgctg ttgctgggga atatggacgc
    10201 ctagcgtccc ggtcgttgac ctgcggcccg cggctgagta ctatatagac cacttctcgg
    10261 ccgcaaggcg gcgcgggcaa taccatggga ggcgattaac atggagatta ccagttggcg
    10321 cgaaaccggc caggtgccgg tcacgatctt gcagctgaaa ggcgatctcg tcgccgaaga
    10381 cccattggga acgcgcgtga ccgcggcttt cgccg
//
LOCUS       DAOSMQ010000045        10327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6467_length_10327_cov_5.292251, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000045 DAOSMQ010000000
VERSION     DAOSMQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10327
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_10327_cov_5.292251"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK829_02680"
     CDS             complement(<1..282)
                     /locus_tag="PK829_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU40151.1"
                     /translation="MDLHLHTPGSNDYQEPHISYLDILRQASARGLDIIAITDHNTVA
                     GYAAMQKEVEQLLWLESRGRIDPLEQRQLDEYRRVLDKLLVLPGFEFTAT"
     gene            complement(412..669)
                     /locus_tag="PK829_02685"
     CDS             complement(412..669)
                     /locus_tag="PK829_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40152.1"
                     /translation="METVDKTLNQELASMDDSRDWERRVVVAGGILGAFVGVVTSLLL
                     IRTSRDAHAGPPAINTSDAFKVGITAIGLVRSIAALGDRQR"
     gene            complement(671..1279)
                     /locus_tag="PK829_02690"
     CDS             complement(671..1279)
                     /locus_tag="PK829_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40153.1"
                     /translation="MPSVRNGGSFATVEPPRSASVYDQTTPGYGYARSTYQPTAEQLA
                     RDEARRAYWRRNVKTPIVISAVLVAALLVVLVLLAFIAPRLGLTTPAARSLIAGLAGL
                     TLILLAIPLIALASILPLAWLALAYRRRDQRKLNPETGPMAYRSRVQTLLWQLDHLLD
                     TAERGVTQGGARVTRPLIGLHARADYWRAFVRAVRRNFTRSN"
     gene            complement(1476..1928)
                     /gene="nusB"
                     /locus_tag="PK829_02695"
     CDS             complement(1476..1928)
                     /gene="nusB"
                     /locus_tag="PK829_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOU40154.1"
                     /translation="MKTRRRARRVTLETLYEYDIANHDPDEVLELRLEASPMERAGVD
                     FAAKLVHGVIEYQTQMDRLIARFAPEWPLDQMAVIDRNVLRIAMYEFLVTDETPVKVA
                     INEAVELAKVYGSDSAPRFINGVLGTLADHLDELKREVRREASLGVTA"
     gene            complement(1986..2345)
                     /locus_tag="PK829_02700"
     CDS             complement(1986..2345)
                     /locus_tag="PK829_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOU40155.1"
                     /translation="MTERQESIGRIEVTPEVLVTIARYAVQEIDGVQQMVPVPNDVAR
                     LFQRGLRQDGVLLDLSENKLRFNIYVIMAPHVNLLETSRAIQHAVSEAIDTMVGIPVD
                     AVNVFVEDVHYNKGEVV"
     gene            complement(2342..3088)
                     /gene="fabG"
                     /locus_tag="PK829_02705"
     CDS             complement(2342..3088)
                     /gene="fabG"
                     /locus_tag="PK829_02705"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOU40156.1"
                     /translation="MLLEGKVAIVTGASRGIGRAIAETLAAEGATVVVNYQSNAAAAA
                     AVVAGIRARGGQALAVAADVSDLAAAEGLIRAALEAYDHIDIVVNNAGTTRDTLLLSM
                     KEEQWDVVIDTNLKSVFNVCKAAARPMLRRKKGGRIINISSVAGIVGQPGQTNYAASK
                     AGIIGFSKSLAKELGSRGITVNVVAPGFVPTDLTAGLPEDLVKQTLAYIPLGRWGEAQ
                     EVAHAVAFLASDRAAYITGEVLRVDGGIGT"
     gene            complement(3190..4092)
                     /gene="fabD"
                     /locus_tag="PK829_02710"
     CDS             complement(3190..4092)
                     /gene="fabD"
                     /locus_tag="PK829_02710"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOU40157.1"
                     /translation="MSTAFLFPGQGSQFVGMGRALYEREPVARALYDEADALLGFALS
                     RLCFEGPEEALTDTAVQQPALFTTSLAAWAVLSARGAADAAYVAGHSLGEFSALAAAG
                     ALTFAGGLALVRRRGELMKLAGERAPGGMAAILGLDAAPVAEVCTAVAAATGRFVGVA
                     NDNCPGQVVITGDETALTEAMRQLAAAGARKVVRLPISIAAHSPLMAAVAAEFAAAVD
                     AAPLAPARVPIVANVTAAPITAPDAIRAELKAQLTAPVQWTNSVRALSAAGVTAYVEV
                     GPGDVLLSLVKRIDRDATRTKFEA"
     gene            complement(4183..4368)
                     /gene="rpmF"
                     /locus_tag="PK829_02715"
     CDS             complement(4183..4368)
                     /gene="rpmF"
                     /locus_tag="PK829_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU40158.1"
                     /translation="MGAVPKRKVSKARRDKRRTHDALSLYHLVPCPDCGEMRRAHHVC
                     PNCGKYNGRQILPEREE"
     gene            complement(4481..5425)
                     /locus_tag="PK829_02720"
     CDS             complement(4481..5425)
                     /locus_tag="PK829_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOU40159.1"
                     /translation="MSDIAKRVVLTGATGFVGSAVAAELLSHGHELVVFSRDPQAAAA
                     KVPGAAEYVAWQPEASGLWAAAVDGADAVVHCAAASIFDRRYTRQVAHDALANRVIST
                     RGLVNAMAQAAHKPRVFVNSASQGIYGFEGVSQAEVDERTPPATDYWGQDSKPWEDEA
                     LRAESLGIRTVVMRIGYVLGADGGGLPYQVRQARKGQSGISRPAEASLSWIHIRDLAR
                     LFRFAVENASLHGPVNGTAPQPASNGEYAELLGQLTGQPVKLMPYPLVRLFVGKVAEI
                     YARQKRVIPRQALAAGFTFDYPTLAEALADLAPRVSEA"
     gene            5710..6633
                     /gene="cyoE"
                     /locus_tag="PK829_02725"
     CDS             5710..6633
                     /gene="cyoE"
                     /locus_tag="PK829_02725"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOU40160.1"
                     /translation="MSFLKATERTFEGQMTTAGWRSTLHTVMVLFKLRIVFLLLMAAT
                     GGSFLAAGGWPGWRALLLVWVTGGMAAAGASALNQYWERNRDGLMGRTRHRPLVNGDI
                     TDPRWVPIAGVALVLVPVLAVLPFNRPLAFFLLLGAFIYVGIYTIWLKPRTLLNIVIG
                     GAAGSAAVLSGSAAAGAWNAVGALALAAILFLWTPFHFWSLAILYRDEYSRADVPMLP
                     TQTTPHRAAWWVMTHTLPTGILSLLLVVLPGLSWLYFLPVLAITIDLFYRNVQLIRDP
                     SPQYARKLFMSSNYYLLVLLLAICVDLALPW"
     gene            6990..8267
                     /locus_tag="PK829_02730"
     CDS             6990..8267
                     /locus_tag="PK829_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU40161.1"
                     /translation="MKLRVFVGLAVLLLALALVACGGGDDTSTITPVAEATTAPAATE
                     ATTAEEPAATEEPAAEEPTATAEAVAEQPTATEAAAATEEPAATEPPAATADPAALPT
                     QAVFVGGGPTLEAVRNRGVLRCAGNQAVPGFGYINPDTNEFEGFDIDYCKAIAVAVLG
                     EPAFEVRHTSANERFPVLQSGEVDVLVRNTTWTISRDTSVGLNFAPVTFYDGQGMMVR
                     TDSGITDLEGLDGGTICVQSGTTTEKNLADVFRARGIDFTPVVFEDADATREAYDQGQ
                     CDGFTTDKSGLVSQQILLADPTAHIILDETMSKEPLAPAVRQGDDNWYDIVKWTVYCT
                     IDAEELGISSENVDTFLGGEDPVIQNLLGETGDLGLAMSLNADFCYQVIKQVGNYGEI
                     YNRNLGPDTPFNLPRGLNNLYTNGGLQYSPPFR"
     gene            8341..9954
                     /locus_tag="PK829_02735"
     CDS             8341..9954
                     /locus_tag="PK829_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119978.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU40162.1"
                     /translation="MTDLAPKTTRRAAETPFWRDARVLGVLAQAVFLAVVVAAAAWIG
                     SNASQNMLRLGGSQFLCRDGTTSFRCAFDFLRLDAQFAISESLIPYDPSDSYARALAV
                     GALNTAKVAVLGIVLATVLGTVTGIARLSSNWLISTVARWYVDLIRNTPLLVQLFFLF
                     FGVIVLFPPIRAALQPFGLPIYLSQRGINLPRPVVMPSFGTWLIFLVAGVGLAAVAWF
                     LLGRREVRTGQAINRPLWAALAFAVVAVGGWFVAAAGGANNQGFLVSDSAGISVLDDL
                     ATTTAVRLGLVDLDELPGALASGALTEDEVEQGTLIFCGVKDAPAEANLTAQLRRAGV
                     PFAFERFPGPNEAIGGYVDGDCEALVAPRAVLMAASIPASTIVPVPETPLRFSLPRIE
                     GLNFVGGIKLSPNFAAILIGLVLYTGAFIAEIVRAGIQSVPRGQSEAARALGLSEAQR
                     LRLVVLPQALRVIIPPLTSQYLNLTKNSSLAIAVGFPDLWSVAYTTLNQSGRALQVFL
                     LVMATYLAFSLIISFFLNWYNRRIALVER"
     gene            9956..>10327
                     /locus_tag="PK829_02740"
     CDS             9956..>10327
                     /locus_tag="PK829_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40163.1"
                     /translation="MTTHYRNLQLVLRGAADPPFNWREFIHENILHTPWLALWLLALT
                     YIAARVAWRFLVDAPVTTAIILLAWALSLGATALGNILHRHTPATFWLARNLYSSITN
                     ALVTLLLVLLLVAGARGFIDWA"
BASE COUNT         1686 a         3383 c         3407 g         1851 t
ORIGIN      
        1 tgtggcggtg aactcgaagc cgggcaggac gagcagcttg tccagcacgc gccggtactc
       61 atccaattgg cgctgctcca gcgggtcgat gcggccgcgg ctctccagcc acagcaattg
      121 ctcaacctct ttctgcatgg ccgcatagcc ggcgacggtg ttgtggtcag tgatggcgat
      181 gatgtccagg ccgcgggctg acgcctgacg gaggatgtcg aggtaactaa tgtgcggctc
      241 ctggtagtcg ttcgagccgg gggtgtgaag gtgaaggtcc atccgccgcc attggggcga
      301 aggacgcttg gggggccggg ccgcaaccgg gcgagccgat tgggaagccc cgcgggatcg
      361 gccgcgttgg gtcgatccgg gatcattggt catgcgattt gctccttgca attatcgttg
      421 tctgtctccc agagcagcaa tggagcgcac cagaccgatg gcggtgatgc caaccttgaa
      481 ggcgtcgctg gtgttaatgg ccggcgggcc ggcgtgggcg tcgcgtgagg tgcggatcag
      541 cagcagcgag gtgacgacgc cgacaaatgc accgaggatg ccgccagcaa cgacaacgcg
      601 gcgttcccaa tcacgggaat cgtccatcga ggccagctcc tggttcagcg tcttatcgac
      661 tgtttccatt tcagttgctc cgtgtgaagt tgcgccggac agcgcggacg aaggcgcgcc
      721 agtagtcagc gcgggcgtgc aggccgataa gcggccgggt gacgcgcgcg ccgccctggg
      781 tcacgccccg ttcggctgtg tccagcaagt ggtcaagttg ccacagcagc gtctgcacgc
      841 ggctgcggta ggccatcggc cctgtctccg ggttgagctt gcgctggtcg cggcggcggt
      901 aggccagggc caaccaggcc agcggcagga tgctcgccag ggcgatcagg gggatggcta
      961 gtaggataag ggtcagcccg gccagcccgg cgataagcga ccgcgccgcc ggcgtcgtca
     1021 ggcccaggcg cggggcgata aaggccagca gcaccaggac gacgagcagc gcggcgacga
     1081 gcacggcact gatgacgatg ggcgtcttga cgttgcgccg ccagtaggcg cggcgggcct
     1141 cgtcgcgggc cagttgttcg gccgtgggct ggtaggtcga gcgggcatag ccgtagccgg
     1201 gcgtcgtctg atcgtagacc gacgccgagc gcggcggttc gaccgtcgcg aacgagccgc
     1261 cgttacggac ggagggcata ttcgcctgtg gggtgggccg gggcggtgta tttgctgcgg
     1321 tcatggtgag aaagctaatt gagggattat agcacaaagg ggggacaagt gctggccacc
     1381 tgtcaggatc aagagcgggc cgcgcctgga atggccgaat cgctacaaag aaaacaggcc
     1441 ggccggcctg cttgcatccg tcgggaggcg tgcgcctaag cggttacgcc aagcgacgcc
     1501 tcgcgccgca cctcgcgctt gagttcgtcc aggtgatcgg ccagcgtgcc caagacgcca
     1561 ttgatgaagc ggggggcgct atcgctgccg taaaccttag ccaactcgac cgcttcgtta
     1621 atggccacct tgactggcgt ctcgtccgtc accagaaact cgtacatagc gatgcgcagc
     1681 acgttgcggt cgatgacggc catctgatcc agcggccact ccggggcgaa acgggcgatg
     1741 agccgatcca tctgcgtctg gtactcgata acgccgtgaa ccagtttggc ggcgaagtcg
     1801 accccggcgc gttccattgg actggcttcc aaccgcaact ccagcacttc gtccggatcg
     1861 tggttggcga tgtcgtattc gtacagcgtt tctaaggtta cgcgacgcgc ccgacgcctg
     1921 gtcttcattc tcttaacctt ccgggaacct ggttctagcc gccgaccagg ccctcatcag
     1981 ggagcttaaa caacttctcc cttgttgtaa tggacatctt caacaaacac attcacggca
     2041 tcgaccggaa tgcccaccat ggtgtcgatc gcctcggaga ctgcatgttg aatcgcgcgg
     2101 ctggtctcca gcaggttcac gtgcggagcc attataacat agatattgaa gcgtaacttg
     2161 ttttcgctca aatctaacaa aacgccgtcc tgccgcaggc cacgttggaa gaggcgggcg
     2221 acgtcgttcg gcaccggcac catctgttgc accccgtcga tctcctggac ggcatagcgg
     2281 gcgatggtca ccagcacttc cggcgtcacc tcgatgcggc cgatgctttc ctgtcgttcg
     2341 gtcatgtccc gattcctcca tctacgcgca acacttcgcc ggtgatgtag gccgcccggt
     2401 cggaggccag gaaggccacg gcatgggcca cttcctgcgc ctcgccccag cggccgaggg
     2461 ggatgtaggc caatgtctgc ttcaccaggt cttccggcag gccggcggtc aggtcagtgg
     2521 gcacgaagcc gggggcgacg acgttgacgg tgatgccgcg cgagccgagt tccttggcca
     2581 gcgacttgga aaagccgatg atgcccgcct ttgacgcggc gtagttggtc tggcccggct
     2641 ggccgacgat gccggcgacg gacgagatgt tgatgatccg cccgcccttc ttgcgccgca
     2701 gcatcggccg ggccgccgcc ttgcagacgt tgaagacgct cttcaggttc gtgtcgatga
     2761 ctacatccca ctgctcctct ttcatcgaca ggagcagcgt gtcgcgggtg gtgccggcat
     2821 tgttgacgac gatgtcgatg tggtcgtagg cctccagcgc ggcgcggatg aggccctcgg
     2881 ccgcggccag gtcgctcacg tcggcggcca cggccagcgc ctggccgccg cgcgcgcgaa
     2941 tgccggcgac gaccgccgcg gccgcggcgg cgttcgattg gtagttgaca accacggtcg
     3001 cgccctcggc ggccagcgtc tcggcgatgg cccggccaat gccccgcgag gccccggtga
     3061 caatggcgac tttgccttct agtaacatac tcatacccag aatggagaga ttagcgcaaa
     3121 ggcgcaagga cgcaacaaac acctaaatcg tttgcgtctt tgcgtccttg cgcctttgcg
     3181 tgagcttctt tacgcctcga acttggtgcg cgtcgcgtcg cggtcgatgc gtttgaccag
     3241 gctgagtagc acgtcgcctg gcccgacttc gacgtaggca gtgacgcccg cggccgacag
     3301 ggcgcgcacg gagttggtcc actgcaccgg ggcagtcagt tgcgccttca gttcggcgcg
     3361 gatggcgtcg ggggcggtga tcggcgcggc ggtgacgttg gcgacgatgg ggacgcgggc
     3421 cggggccagc ggcgcggcgt ctacggcggc ggcgaattcg gcggccacgg cggccatgag
     3481 gggggagtgg gcagcgatgg agatgggcag gcggacgacc ttacgcgccc cggcggcggc
     3541 cagttggcgc atcgcctcgg tcagcgccgt ctcgtcaccg gtgatgacga cctggccggg
     3601 gcagttgtcg ttggccacgc cgacgaagcg gcccgtcgcc gcggcgacgg cggtacagac
     3661 ctcggccacc ggcgcggcgt ccagtcccag aatggcggcc atgccccccg gcgcgcgttc
     3721 gccggccagt ttcatcagct cgccgcgccg ccgcaccagg gccaggccgc cggcgaaggt
     3781 cagcgccccg gccgcggcca gggcggagaa ctcgcccagc gagtggccgg cgacgtaggc
     3841 cgcatccgcc gcgccgcgcg ccgagagcac cgcccaggcg gccaggctgg tggtgaagag
     3901 cgccggctgc tggacggccg tgtctgtcag ggcctcttcc ggcccctcaa agcacagccg
     3961 cgacagggca aagccgagca gggcgtcggc ctcgtcgtag agggcgcggg cgacgggttc
     4021 gcgctcatag agagcgcggc ccatgccgac aaattgggaa ccctggccgg ggaagagaaa
     4081 ggcggtagac attcaataac gaaggatgaa ggcggaggga tgaaggatga acgctgcgct
     4141 ggcaacattc ctccctcatc cttccgcctt catccttcat tcctactcct cgcgttcggg
     4201 caggatttgc cggccgttgt acttgccgca attcgggcag acgtgatgcg cgcggcgcat
     4261 ctcgccacaa tcggggcagg ggacgagatg atagaggctc aacgcatcgt gcgtgcgccg
     4321 cttgtcacga cgcgctttcg acacttttcg cttgggaaca gcacccatga tgtcacttcc
     4381 ttaaagtatg cagctgcaaa ttcgcgcggg gcgatagacg acataaaacg agcggccaac
     4441 agagccgcga agagtgatta tagccgttgg gggcgcggtg tcaagcctcg ctgacgcgcg
     4501 gcgctagatc cgccagcgct tctgccaggg tgggatagtc gaaggtgaag ccggccgcca
     4561 gggcctggcg cgggatgacg cgcttctggc gggcgtagat ctccgctacc ttgccaacga
     4621 acaaccgcac cagcggatag ggcatcagtt tgaccggctg gcccgttagc tggccgagca
     4681 gttcagcgta ctcgccgttg ctggctggtt ggggcgcggt gccgttgacc ggcccgtgca
     4741 atgatgcgtt ctcgaccgca aagcgaaaca gacgcgccaa atcccggatg tggatccacg
     4801 aaaggctggc ttcggcgggc cggctgatgc cactctggcc cttgcgggcc tgccgcacct
     4861 ggtaggggag gccgccgccg tccgcgccaa gcacgtagcc aatgcgcatc acgaccgtgc
     4921 ggatgccaag cgactccgcc cgcaacgcct cgtcctccca cggcttgctg tcctggcccc
     4981 agtagtcggt ggccggcggc gtgcgctcat cgacctcggc ctggctgacg ccttcaaacc
     5041 cgtagatgcc ctgcgaagcg ctattgacga aaacgcgcgg cttgtgggct gcttgcgcca
     5101 tcgcattgac cagcccgcgc gtgctgatga cgcggttggc cagcgcgtcg tgggcaacct
     5161 gtcgggtgta gcgtcgatca aagatggacg ccgcggcgca gtggacgacg gcgtctgccc
     5221 cgtcgacggc cgccgcccac agcccgctcg cctctggctg ccaggcgaca tactcggccg
     5281 cgccgggcac tttggccgcc gcggcctgcg ggtcgcgcga gaagaccaca agctcatggc
     5341 cgtgcgagag aagttccgca gccacggccg agccgacgaa gcccgtcgcg cccgttaaaa
     5401 caactcgctt ggctatgtca gacaatgatg cagctcctta cctacgcgat tggcttcgct
     5461 cagcgtcagc caatgggttt caattgccaa ccaatcatta tacccctctt ctggcggttt
     5521 tacactctgc caatgatgcc agccacaccg tttttccaat tgtcattggc tgatttgccc
     5581 ccgataggcg actccagaaa agccctggcg cgcgccacgg tgcgtttgtt tcctgcgccg
     5641 gtggttgcta caattacgcg atcgccaaaa gggcggttgg gtgcgggtgt cattcacgct
     5701 ggagaggtta tgtcgttttt aaaggcaacg gaacggacat ttgagggcca gatgactacg
     5761 gccggctggc gcagcacctt gcacaccgtc atggtgctgt tcaagctgcg catcgtcttt
     5821 ttgctgctca tggcggctac tggcgggtcg tttctggccg ccggcggctg gccggggtgg
     5881 cgggcgctgc tgctggtctg ggtgacgggc ggcatggccg cggccggcgc gtcggcgctg
     5941 aaccagtatt gggagcgcaa ccgcgatggc ctgatgggcc gcacacgcca ccggccgctg
     6001 gtcaatgggg atatcaccga tccccgctgg gtgcccatcg ccggggtggc gctggtgctc
     6061 gtgcccgtgc tggcggtgct accgtttaac cggccgctgg ccttcttcct gctgctcggt
     6121 gcgttcatct acgttggcat ctacacgatc tggctgaagc cgcgcacgct gctcaatatc
     6181 gtcatcggcg gggcggccgg cagcgccgct gtcctcagtg gcagcgccgc cgcgggagcg
     6241 tggaacgccg tcggcgcgct ggcgctggcg gccatcctgt tcctgtggac gcccttccac
     6301 ttctggagcc tggccattct ctaccgcgac gaatacagcc gcgccgacgt gcccatgttg
     6361 cccacgcaga cgacgcccca ccgggcggcc tggtgggtca tgacccacac gctgccaacg
     6421 ggcatcctca gcctgctgct ggtggtcttg cccgggctga gctggctcta cttcctgccc
     6481 gtgctggcca tcaccatcga cctcttctat cgcaacgtcc agctcattcg cgacccttcg
     6541 ccgcaatacg cgcggaagtt gttcatgtcg tctaactact acctgctggt attgctactg
     6601 gccatttgcg tcgacctggc cttgccgtgg taggccgtgg gcgggagcgg ggcggccagt
     6661 gatttgcgca ccggcgcgcc atcgtctata gtacccgccg caatcgacaa actggttggc
     6721 tcatcgccac cggctgacaa cgggccggtc accgctgctc acgcggatcg gtttgcccac
     6781 cgccccgcgt aatatcatcg ccgccatcat gctttgagac gcagggccgc ccacccaggc
     6841 ggccaaatag aatagcttgc caagcggcgc gaccgacaaa ccggccgccg cttgtcgcag
     6901 gaaggagcac cgccgggcaa gggaaacggc gcattggcac tttaacatgg cgtgaaatag
     6961 atcgagttga cgagaggaga aggaaggtca tgaaattgcg agtgtttgta ggcttggctg
     7021 tgctgttgct agcgttggcc ctggtggcct gcggcggcgg cgacgatacg tccaccatta
     7081 ccccagtagc cgaggcgacg acggccccgg cggccaccga ggccactacg gccgaggagc
     7141 cggcggccac cgaagaaccg gccgccgaag aaccgacggc caccgcagag gccgtcgccg
     7201 agcagccgac ggcgaccgaa gcagcggcgg cgacagaaga accggcggcc actgagcctc
     7261 ccgcggcgac ggccgacccg gccgccctgc ccacccaggc cgtctttgtc ggcggcggcc
     7321 ccacgctgga agccgttcgc aatcgcggcg tcctgcgctg cgccggcaat caggccgtgc
     7381 ccggctttgg ctacatcaac cccgatacga atgagtttga gggctttgat atcgactact
     7441 gcaaggccat cgccgtggcc gtgctgggcg aaccggcctt cgaagtgcgc cacaccagcg
     7501 ccaacgagcg cttccccgtc ttgcagtcgg gcgaggtcga tgttctggtg cgcaacacca
     7561 cctggacgat cagtcgcgat acgtctgtgg gcctcaactt cgccccggtg acgttttatg
     7621 acggccaggg catgatggtg cgcaccgact ccggcatcac cgaccttgaa gggctagacg
     7681 gcggcacgat ctgcgtccag tcgggcacga ccacagagaa gaacctggcc gatgtcttcc
     7741 gcgcccgcgg catcgatttc acgccggttg tcttcgagga cgccgacgcc acccgcgagg
     7801 cttatgacca gggccagtgc gacggcttca ccaccgacaa atccggcctc gtttctcagc
     7861 agatcctgct ggccgacccg acggcccaca tcatcctcga cgaaaccatg tcgaaggagc
     7921 cgttggcccc ggccgttcgt cagggcgacg acaactggta cgacattgtc aagtggacgg
     7981 tctactgcac catcgacgcc gaggagttgg gcattagttc cgagaacgtc gataccttcc
     8041 tgggcggcga agacccggtc atccagaatc tgctgggcga gaccggtgac ctgggcctgg
     8101 ccatgagcct gaacgccgac ttctgctacc aggtcattaa gcaggtgggc aactacggcg
     8161 agatctacaa ccgcaatctc ggtccagaca cgccgttcaa cttgccgcgc ggtctgaaca
     8221 acctctacac caacggcggg ttgcaatact cgcccccgtt ccgctaggag ctagacgcac
     8281 agggggccgg tgcccggccg gcccctttgt gtttcgccgg ccaacggaag ggggttatcc
     8341 atgacagacc tagcgcccaa aacgacacgg cgcgcggcag agacgccgtt ctggcgcgac
     8401 gcgcgcgtcc tgggtgtcct ggcccaggcg gtctttctgg ccgtcgtcgt cgccgcggcc
     8461 gcctggatcg gcagcaatgc ctcgcagaac atgctgcggt tgggcggcag ccagttcctc
     8521 tgccgcgacg gcacgaccag cttccgctgc gccttcgact tcctgcggct cgacgcccag
     8581 ttcgccatct ccgagtcgct catcccctat gaccccagcg acagctacgc gcgggcgctg
     8641 gccgttggcg cgctgaacac ggccaaggta gccgtgctgg gcatcgtcct ggcgacggtg
     8701 ttgggcacgg tgaccggcat cgcccggcta tcgtccaact ggctgatcag caccgtcgcc
     8761 cgctggtacg tcgatctcat ccgcaataca cccctgctgg tgcagctctt cttcctcttc
     8821 ttcggcgtca tcgtgctctt cccgcccatt cgcgcggcgc tgcaaccgtt cggtctgccc
     8881 atctacctga gccagcgcgg cattaatcta ccccggccgg ttgtcatgcc ctctttcggc
     8941 acgtggctga tctttctggt cgcgggggtg gggctggcgg ccgtggcctg gttcctgttg
     9001 ggccggcgcg aggtgcgcac cgggcaggcc atcaaccggc cgctctgggc cgcgctggcg
     9061 ttcgccgtgg tggccgttgg gggctggttc gtggctgccg ccggcggggc caacaaccag
     9121 ggcttcctcg tatccgattc ggccggcatc agcgtgctcg acgacctggc cacgacgacg
     9181 gccgtgcggc tgggcctggt cgatctcgac gagttgcccg gcgctctggc cagtggcgcg
     9241 ctgactgagg acgaggttga gcaggggaca ctcatcttct gtggcgttaa ggacgcaccg
     9301 gccgaggcca acctgaccgc acagctgcgg cgggcgggcg tgccctttgc gttcgagcgc
     9361 ttccccggcc cgaacgaggc catcggcggc tatgtcgatg gcgactgcga ggcgctggtg
     9421 gccccacggg ccgtgctgat ggccgcgtct atccccgcca gcaccatcgt gcccgtgccg
     9481 gagacgcccc tgcgcttctc tctgccgcgc atcgaggggt taaacttcgt cggcggcatc
     9541 aagttgtcgc ccaacttcgc cgccatcctc atcggcctgg tcctctacac cggcgcgttc
     9601 atcgccgaga tcgtccgcgc cggcatccag tccgtaccgc gtggccagag cgaggccgcg
     9661 cgggcgctgg gcctgagcga ggcgcagcgg ctgcggctgg tcgtcttgcc ccaggcgctg
     9721 cgggtcatca tcccgccgct gaccagtcaa tacctcaacc tgaccaagaa ctccagcctg
     9781 gccatcgctg tgggcttccc cgatctgtgg tcggtggctt acacaacgct gaaccagtcg
     9841 ggccgggcac tgcaagtgtt cttgctggtc atggccacct atctggcctt cagtctgatc
     9901 atctcattct tcctgaactg gtacaaccgg cgaatcgccc tggtggagcg ctgagatgac
     9961 gacgcactac cgcaacctcc agctcgtcct gcgcggcgcg gccgacccgc cgtttaactg
    10021 gcgcgaattc atccacgaga acatcctcca cacaccctgg ctggcgttat ggctgctggc
    10081 gctgacgtat atcgccgcgc gcgtggcctg gcgcttcctg gtcgacgcgc cggtgacgac
    10141 ggccatcatc ctgctggcct gggcgctgtc gctgggagcc acggccctgg gcaacattct
    10201 ccaccgccac acgccggcga ccttctggct ggcccgcaac ctctatagct ccatcaccaa
    10261 cgccctggtg acgctgctgc tggtgctgct gctggtggcc ggggcgcgcg gcttcatcga
    10321 ctgggcc
//
LOCUS       DAOSMQ010000046        10276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6511_length_10276_cov_4.657274, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000046 DAOSMQ010000000
VERSION     DAOSMQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6511_length_10276_cov_4.657274"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PK829_02745"
     CDS             <1..1167
                     /locus_tag="PK829_02745"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOU40164.1"
                     /translation="MAALDPDRYEVVPIGITKAGRWLAGDAMAALSDEAGAARPATLL
                     PEPSAGKAATSALLALDRDTRSGADATPNLSVVAELDVVFPVLHGPYGEDGTVQGLLE
                     LAGLPYVGAGVVGSAVGMDKAIFKQVMEANGLPVLPWRLFTGSQWRHHPEAVLDAVED
                     ALSYPVFTKPANLGSSVGISKCRDRAQLRAGLAAAARFDRRLIVEQGIDRARELEVAV
                     LGNDEPRASVVGEVRPRREFYDYVAKYLLAPGSEEESELLIPAPLEPALAEAIRALAV
                     RAYRAIDCAGLGRVDLLLDDHSGDIYLNELNTIPGFTSISMYAKLWAASGLSYSALLD
                     ELIDLALQRHAEKADLETAIDPTAETSAALSDHSAIRDDRASPTAPQPAEGATE"
     gene            1164..2168
                     /locus_tag="PK829_02750"
     CDS             1164..2168
                     /locus_tag="PK829_02750"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOU40165.1"
                     /translation="MKQRQEQPKPRLRKQPRMRRLNTASVAPLPALTPEARTAQKKAQ
                     KRKRRNSQERLRRPLSGIVGLIVNTRWISLAVLAACVYTLYIIGSDDRFYLNVIPVEG
                     ASSLDINHVVEESGLAGQHIFAADPQEAADRLSEMGGVVTATVTLRWPNDVLITLREQ
                     PPLATWQEGDQSYWVDADGGLSPARAQTIGLLNIISEIPVAADGAPAAKTMTADDSNA
                     GAIVAAEEDASDEAGATADPGDGADAQIAFLPQDVLEGALQLRSLRPNIEQLYYRPSG
                     GLSYQDGRGWRAYFGTGRDMNQKLAVYETVVAKLLEQGIRPEYISVSNQYKPYYRALP
                     "
     gene            2208..3440
                     /gene="ftsA"
                     /locus_tag="PK829_02755"
     CDS             2208..3440
                     /gene="ftsA"
                     /locus_tag="PK829_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU40166.1"
                     /translation="MEDIVFGIDIGTTKICALVGAVRNRELQIIGLGVQPSSGMRKGM
                     IVDVPSATVALARAVQEAEETSGYRLSQAVVSIAGEHIGSTNNAGMVAIGQSGARGAD
                     TGVTTADIDRALETAQAIPIPHNRQIIHLIPRHYTIDDHAGVRQPLGMHGYRLEVEAH
                     IVTAATSALQNLQHCVGGVGINAAETVLNALASAEAVLEPAEREMGVLIADIGGGTTD
                     LALYKQGTVWHTHVIPIGGYHITNDIAIGLRAPYETAEKVKLQYGDCRPADIDPDLVF
                     TVEPFGGEKIQVGRQDLAQVIEARIEEIFNLILKDIQQSGYDELLPAGIVLTGGTAQL
                     RGITSVAERVLNVPARVATPKNLVGLVDTLHSPAYATSVGLLRWASTDHHRYQARTQA
                     PSEWSRRVGNLIRTLLPD"
     gene            3546..4709
                     /gene="ftsZ"
                     /locus_tag="PK829_02760"
     CDS             3546..4709
                     /gene="ftsZ"
                     /locus_tag="PK829_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU40167.1"
                     /translation="MPATEGSALIRVVGVGGGGSNAVNRMIAENIAGVEFVAVNTDQQ
                     ALLGSHAPIRIAIGERTTRGLGSGGDPETGARAAEESLEELRQSLSGSDMVFVTAGMG
                     GGTGTGATPIVARAAREAGALTIGVVTRPFTFEGSQRARSAETGIEQLKEHVDTLIVI
                     PNDRLLETADKRVSLLDSFRMADDILRQGIQGISELITVPGLINLDFADVKTIMSMGG
                     AALMAVGEGTGDERARDAATQALSSRLLDVTIDGARGILFNVTGGPDMTLYEINQAAA
                     IIRETAHPDVNLIFGAVIDEKMTNKIRITVIATGFEHTMPMRQMTAATASQTQRLMPN
                     RPPTRESAPVGAPVSGPTGQAAARSQPQQPARQNQEAYRFNDLEVPAFLRKRQ"
     gene            4862..5317
                     /gene="nrdR"
                     /locus_tag="PK829_02765"
     CDS             4862..5317
                     /gene="nrdR"
                     /locus_tag="PK829_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOU40168.1"
                     /translation="MRCPYCGYEKTHVIDTSHDRRGGTRRRRVCEACDQRFTSYERPI
                     LATPLLIKKDGTREEFSREKLLGGIRVACAKRPISAADIERMAGEIEAELQRMGQEEV
                     KSRIVGDMVIRKLKELDLVAYVRYAIVYLRLDDLTSIRGEIDRLLTEQH"
     gene            complement(5447..7870)
                     /locus_tag="PK829_02770"
     CDS             complement(5447..7870)
                     /locus_tag="PK829_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU40169.1"
                     /translation="MARTHRPLSLLLVLIPVALVAARWGRLAAAPVAAPAVLIDSVLF
                     DGYALDDADEAVRLVNAGAAPADLSGWRLGDGADVALIPGGTSLAPGATLWLARDAAA
                     FRAQFGHDADLQLAAWPGYSNSGDEVVLADATGQPVDVLVYLDGDTGRGGWTGPSLAP
                     YRVAGLFAAEGQILFRRRDEATGRPVADTDTAADWGQYTGDPIAGRKVRYAGWAVERF
                     AAPAVITATGTLTVAVAPDNAYDVLTRLIDRAAISIRLSSLTLEHAGLGMALARAAGR
                     GVAVTALLDGAPPGGLSNQERYVCAGLEAAGGACWFMTGDVASRVHSRYRYLHAKYLV
                     VDDHIAAVSSENFSPDSLPDDDKADGTWGRRGVVLITDARAVAQYLATLFATDLDTVH
                     ADLRRWSAADPIYGAPPADFAPITASGGLSYTVRFPQPAVFFGTHRFEIIQSPENSLR
                     AGDGLLALLGRAGQGDALMFEQLSERPHWGATGSTAAADPNPRLEAALAAARRGADVA
                     LLLDSFFDDAGSAVSNEATCSYVNEVSAREGLRLRCATGNPTGLGIHNKMVLARIGGR
                     GVVHVGSLNGTELSHKANRELAVQVWSDGAYAYLARVFAGDMTNRLYLPLALAGAQGA
                     ADHLLISEVVYDPPGPDEAEFVELVNPTGAPIDLTGYALGDAVSALDFEDMRHFPPGA
                     VLPAGGVAVVALSAAAFQAEYDVTPQFEIVDSDPTVPDLLDDPAWGDPAALFQLGNAG
                     DEVVVIRWDGLVDVVTYGDGYHAGVIGCGLLVAPARTLERRPYWRDTDSCPADFRAWP
                     FPSPGSVPP"
     gene            complement(7922..8830)
                     /locus_tag="PK829_02775"
     CDS             complement(7922..8830)
                     /locus_tag="PK829_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOU40170.1"
                     /translation="MRILFFGLPAGVSAVVLRGLLADGRAVAGVVVPAASVPHLLPDP
                     VPPIARLAPPAAAPLALALGDAPAGLLGCAWAAGLPVFAARALGHAGTLAALAAARAD
                     VACMACFTRRVPPALLALPPRGFLNLHPSLLPAYRGPQPLFWQLREGAPTGATVHYLD
                     TGLDTGDIAAQTAVPLPDGLPGPEAEQRLALAGLALLRGVLDDLAAGVVRRRPQGPGS
                     YQPTPADADFALSAGWPARRAYNFMRGTADWGKPYPIEVNGRTEWLAAADDFTEAELD
                     RPSVRHGRHIAIRCSPGVLYARLLRR"
     gene            8966..9277
                     /locus_tag="PK829_02780"
     CDS             8966..9277
                     /locus_tag="PK829_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HOU40171.1"
                     /translation="MTRETKTLTVVQCAKLGAELPALDRPPFPGKLGQRIYDEISKYA
                     YSLWQDQARLLINHYGLNMADPRSQEFLFEQMEAFLFDEGSPAVSGAPVAGGKGGPAR
                     K"
     gene            9283..>10276
                     /locus_tag="PK829_02785"
     CDS             9283..>10276
                     /locus_tag="PK829_02785"
                     /inference="COORDINATES: protein motif:HMM:NF040546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HOU40172.1"
                     /translation="MSAAPGDVLLLACYELGHQPLSLAWPAAVLRQAGLPVTVADLAV
                     DALPREAVVRARFVGISVPMHTALRLGVRAAAEVRALNPTTHICFYGLYAWLNADYLL
                     ATAADAVLAGELEEELVVQARRVLDAKPAEDNARADRRLTRLALPTPDRAGLPPLSHY
                     ARYVEGGVARLAGTVEASRGCLHTCRHCPVVPVYGGRFFVVPAETVLADIRQQVAAGA
                     EHIAFGDPDFLNGPGHVLKIARALHAEFPRLTFDFTTKVEHIRQHRALFPELRALGAS
                     FVVSAFESVSDEVLARLHKGHTAADLDDALGVLAGAGLAVQPTWLPFTPWTTLDD"
BASE COUNT         1738 a         3609 c         3481 g         1448 t
ORIGIN      
        1 atggccgcgc tcgaccccga ccgctacgag gtcgtgccca tcggcatcac caaggccggc
       61 cgttggttgg ccggcgacgc catggccgcc ctcagcgacg aagccggcgc cgcgcgcccg
      121 gccacgctgt tgcccgagcc gtccgccggc aaggccgcga cctcggcgct gctggccctt
      181 gaccgggaca cccgttccgg ggccgacgcc acgcccaacc tgtcggtcgt ggccgagctg
      241 gatgtcgtct tccccgtgct ccacggccct tatggcgagg acggcacggt gcagggcctg
      301 ctagagttgg ccgggctgcc ctacgtcggc gcgggcgtgg tcggctcggc cgtgggcatg
      361 gacaaggcca tcttcaagca ggtcatggag gccaacggtc tgcccgtgct gccctggcgg
      421 ctattcactg gcagccagtg gcggcaccac ccggaagccg tcctggacgc cgtcgaagac
      481 gctctgtcct accccgtctt taccaagccg gccaacctcg gctccagcgt cggcatcagc
      541 aagtgccgcg accgcgctca gttgcgcgcc ggcctggccg cggcggcccg cttcgaccgc
      601 cgcctcatcg tcgaacaggg catcgaccgg gcccgcgagc tggaagtggc cgtgctgggc
      661 aacgacgagc cgcgcgccag cgtcgtcggc gaagtgcggc cgcgccgcga attctacgac
      721 tacgtcgcca agtatctgct ggctcctggc tctgaggaag agtcggaact gctcatcccc
      781 gccccgctgg aaccggctct ggccgaggcc atccgcgctc tggccgtccg cgcctaccgg
      841 gccatcgact gcgccggcct gggccgcgtc gacctgttgc tcgatgacca cagcggcgac
      901 atctacctga atgaactcaa caccattccc ggcttcacca gtatctccat gtatgccaag
      961 ttgtgggctg ccagcgggct gagctacagc gccttgctcg acgaactcat cgacctggcc
     1021 ctgcagcgcc acgccgagaa ggccgatctg gaaacagcca tcgacccgac ggcggagacg
     1081 tccgccgcct tgagcgacca tagcgcgatt cgggacgatc gcgcctcccc aacggcccca
     1141 cagccggccg aaggagcgac ggaatgaaac agcgccagga acaaccaaag ccgcgtctgc
     1201 gcaaacaacc acgcatgcgc cgcctgaaca ccgcctcggt cgcgcccttg ccggccctga
     1261 cgccggaggc gcgtacggcc cagaaaaagg cccagaagcg caagcggcgc aatagccagg
     1321 agcgcctccg ccggccgctc tccggcatcg tcggcctcat cgtcaacacc cgctggatca
     1381 gcctggccgt gctggccgcg tgcgtctaca cgctctacat catcggcagc gatgaccgct
     1441 tctacctcaa tgtcatcccg gtcgagggcg cgtcttcgct cgacatcaac cacgtcgttg
     1501 aagaaagcgg cctggccggg cagcacattt tcgccgctga cccccaagaa gcggccgacc
     1561 gcctgagcga gatgggcggc gtcgtcacgg cgacggtgac gctgcgctgg cccaacgatg
     1621 tgctcatcac cctgcgcgag cagccgccgc tggccacatg gcaggagggc gatcagtcct
     1681 actgggttga cgccgacggc ggtctgtcgc cggcgcgcgc gcagaccatc ggcctgctga
     1741 acatcatctc ggagataccc gtcgccgcgg acggcgcgcc agctgctaag acaatgaccg
     1801 ccgacgacag caacgccggt gccatcgtcg ccgcggaaga ggacgccagc gacgaagccg
     1861 gtgcgaccgc cgacccgggc gacggcgcgg acgcccaaat cgccttcctg ccccaggacg
     1921 tgctggaggg cgcgctgcaa ttgcgctctc tgcgtcccaa catcgagcag ctctactacc
     1981 ggccgtcagg cggactgagc taccaggacg ggcgtggctg gcgagcctac ttcggcaccg
     2041 gccgagatat gaatcaaaaa ctggccgttt acgagacagt ggtggccaag ctgctggagc
     2101 agggcatccg gccagaatac atcagcgtca gcaaccaata caaaccctat tatcgggcgc
     2161 tgccgtagcc tgtagcgcgg gttggtaacc cgcgagggaa gaagcgtatg gaggacatcg
     2221 ttttcggaat cgacattggc acaacgaaaa tctgtgccct ggtgggcgcg gtgcgcaacc
     2281 gcgagctaca gatcatcggt ctgggcgtgc agccgtcgag tgggatgcgc aaggggatga
     2341 tcgtcgatgt gccctcggcc acggtggccc tggcgcgcgc cgtgcaggag gccgaggaga
     2401 catcgggcta ccgtctgtcg caggccgtgg tcagcatcgc cggcgaacac atcggctcga
     2461 ccaacaatgc tggcatggtg gctatcggcc agagcggcgc ccgcggcgct gacaccggcg
     2521 tgacgacggc cgacatcgac cgtgccctgg agacggctca ggccatcccc atcccgcata
     2581 accgtcagat catccacctg atcccgcggc actataccat cgacgaccat gccggcgtgc
     2641 gccaaccgct gggcatgcac ggctaccggc tggaggtcga ggcccacatc gtcaccgcgg
     2701 cgacgtcggc cctgcaaaat ctgcaacact gcgtcggcgg cgtgggcatc aacgccgcgg
     2761 agacggtgct caacgcgctg gcgtcggccg aggccgtgct ggagccggcc gagcgcgaga
     2821 tgggcgtcct catcgccgac atcggcgggg gcacgaccga cctggccctc tacaagcagg
     2881 gcacggtctg gcacacgcac gtcatcccca tcggcggcta ccacatcacc aacgacatcg
     2941 ccattggcct gcgcgccccc tacgagacgg ctgagaaggt caagctccag tacggtgatt
     3001 gccgcccggc cgacatcgac cccgacctcg ttttcaccgt cgagccgttt gggggcgaaa
     3061 agatacaggt gggccggcaa gacctggccc aggtcatcga agcgcgcatt gaggaaatct
     3121 tcaacctgat cctcaaggac atccagcagt cgggctatga cgaactgctg ccggcgggca
     3181 ttgtgctgac cggcggcacg gcccagctgc ggggcattac cagcgtggcc gagcgcgtgc
     3241 tgaacgtgcc cgcccgcgtg gccacgccca agaacctggt tggccttgtc gataccttgc
     3301 acagcccggc ctacgccacc agcgtcggcc tgctgcggtg ggccagcacg gaccaccatc
     3361 gttaccaggc gcgcacccag gcacccagcg aatggagccg gcgggtgggc aatctcatca
     3421 gaacactgct gccggattaa agggagtccg gcgacagcgc caaaccaagt aagttgtctg
     3481 tcagatcaaa gtcagagggg tatagagaca tgaacgtgga ttacagagca agagacgcgc
     3541 agcgcatgcc ggccaccgaa ggctcggccc tgattcgagt ggtgggcgtt ggcggcggcg
     3601 gcagcaacgc cgtcaaccgc atgatcgccg agaacattgc cggcgtggag ttcgtggccg
     3661 tcaacaccga ccaacaggcc ctcctgggca gccacgcgcc catacgcatc gccattggcg
     3721 agcgcaccac ccgcggcctc ggctccgggg gcgacccgga gacgggcgcg cgggcggccg
     3781 aggagtcgct ggaggagttg cgccaatcgc tatccggctc cgacatggtc ttcgtgaccg
     3841 ccggcatggg cggtggcacg ggcacggggg ccacgcccat cgtcgcccgc gccgcccgcg
     3901 aggccggggc gctgaccatt ggcgtcgtca cacggccgtt caccttcgag gggtcgcagc
     3961 gggcgcgctc ggccgagacg ggcatcgagc agctcaagga gcacgtcgat acgctgatcg
     4021 tcatccccaa cgaccggctg ctggagacgg ctgacaagcg cgtctccctg ctcgactcgt
     4081 tccgcatggc cgacgacatc ctccgccagg gtatccaggg tatcagcgaa ctcatcaccg
     4141 tccccggcct gatcaacctc gacttcgccg acgtgaaaac gatcatgtcg atgggcgggg
     4201 cggcgctgat ggccgtgggc gagggcacgg gcgacgagcg cgcccgcgac gcggccaccc
     4261 aggccctctc cagccgcctg ctagacgtga ccatcgacgg cgcgcgcggc atcctgttca
     4321 acgtcaccgg cgggccagac atgaccctct atgagatcaa ccaggccgcc gccatcatcc
     4381 gcgagacggc ccacccggac gtcaacctca tcttcggcgc ggtcatcgat gagaagatga
     4441 ccaacaagat ccgcatcacc gtcatcgcca ctggctttga gcacacgatg ccgatgcgcc
     4501 agatgacagc ggcaacggcg tcgcagacgc agcgcctgat gcccaaccgg ccgccgacgc
     4561 gcgagtccgc gcccgtcggc gcgcccgtca gcgggccaac cggccaggca gcggcccggt
     4621 cgcagccgca gcaaccggcg cgccagaacc aggaagccta tcgcttcaac gatctggaag
     4681 tgccggcttt cctgcgtaag cgccagtagg cgcggcgcgg cgggccaggc cgttgccgtg
     4741 cagcggctcc gtgatagaat tcggtcgatg gcatatattg gggtctgctc ccaggacagt
     4801 gcctgggaac ccaatatatg ccgtctatac gacccattct ttcccacgga gggcaagacc
     4861 aatgcggtgt ccttattgtg gctacgaaaa gacgcacgtc attgacacca gtcacgaccg
     4921 gcgcggcggc acgcggcggc ggcgggtgtg cgaggcgtgc gatcagcgct ttacgtccta
     4981 tgagcggcca attctggcca cgccgttgct catcaagaaa gacggcacac gcgaggagtt
     5041 ctcgcgcgag aagttattgg gtggcatccg tgtggcctgc gccaagcggc cgatctcggc
     5101 tgccgacatc gagcgcatgg ccggcgagat cgaagctgag ttgcagcgca tgggccagga
     5161 agaggtgaag agccgtatcg ttggcgacat ggtcatccgc aagttgaagg aactggacct
     5221 ggtcgcctat gtgcgctacg ccatcgtcta cctgcgcctg gacgacctga cctcgatccg
     5281 cggtgagatc gatcggctgt tgactgagca gcattagcct ggcagttcgg gccatatggc
     5341 ccttggctga ccgcggcccc gcgccgtgtc tctatccctt caggaagccc tgcgccccgc
     5401 agggcttccg cgcgcccgct ggcccccccg ctctggtcgc cgcgtcctac ggaggcacac
     5461 tgcccggact tggaaacggc caggcgcgga agtcggccgg gcagctatcg gtgtcgcgcc
     5521 agtaaggccg ccgctccagc gtccgcgccg gggcaaccag tagcccgcag ccgatgacac
     5581 cggcatgata gccgtcgcca taggtgacca catctaccag cccatcccag cgaatgacca
     5641 cgacctcgtc gccggcgttg cccagttgga acagcgccgc cgggtcgccc caggcggggt
     5701 cgtcgagcag gtcgggcacg gtcgggtcgc tgtcgacgat ctcgaattgt ggcgtaacat
     5761 cgtattccgc ctggaaggca gccgcgctga gggccacgac ggccacaccg cccgccggca
     5821 agaccgcgcc gggcgggaag tggcgcatgt cctcgaagtc cagcgccgaa acggcgtccc
     5881 ccagggcata gccggtcagg tcgataggcg cgccggtggg gttaaccagc tcgacgaact
     5941 cggcctcgtc tggccccggc gggtcgtaga cgacctcgct gatgagcagg tggtcggccg
     6001 cgccctgagc accggccagc gccagcggca ggtacaaccg gttggtcatg tcgccggcaa
     6061 agacacgggc cagataggca taggctccat ctgaccacac ctgcacagcc agctcgcggt
     6121 tggctttgtg cgatagctct gtgccattga ggctgccgac gtggacgaca ccacggccgc
     6181 cgatccgcgc cagcaccatc ttgttgtgga tgcccagccc ggtcgggttg cccgtcgcgc
     6241 agcgcagccg cagtccctcc cgcgcggaga cttcgttgac ataactacaa gtggcctcat
     6301 tactaacggc gctgccggca tcgtcgaaga aactatcgag caacagggcc acgtccgcgc
     6361 cgcggcgggc ggcggccagg gcagcctcca ggcgcgggtt ggggtcggcc gcggcggtgc
     6421 tgccggtcgc gccccagtgg ggccgctcgg agagctgctc gaacatcagc gcgtccccct
     6481 gccccgcccg gcccagcagc gccaacagcc cgtcgccagc gcgcaggctg ttctccggcg
     6541 actggatgat ctcgaagcgg tgtgtgccga agaacacggc tggctgcggg aagcgcacgg
     6601 tgtagctgag gccaccgctg gcggtgatgg gcgcaaaatc agccggcggc gcgccgtaga
     6661 tggggtcggc cgccgaccag cggcgcaggt cggcatggac tgtatccaga tcggtggcaa
     6721 agagggtggc caggtattgg gccacggcgc gggcatcggt gatgagcacg acgccgcgcc
     6781 gcccccatgt gccgtccgcc ttgtcgtcgt cgggcaggct gtcggggctg aagttctcgc
     6841 tgctgacggc ggcgatgtgg tcgtcaacga ccaggtactt ggcgtgcagg tagcggtagc
     6901 ggctgtggac gcggctggcg acgtcgccgg tcatgaacca gcacgcccca ccggcggcct
     6961 ccagccccgc gcagacgtag cgctcctggt tgctcagccc acccggcggc gcgccatcaa
     7021 gcagcgccgt cacggccacg ccccgcccgg ccgcccgcgc cagggccatg cccaggccgg
     7081 catgctccag cgtcagcgag gataggcgaa tggagatcgc cgcccggtcg atgagccgcg
     7141 tcaggacatc gtaggcattg tcgggggcca cagccacggt cagcgtgccg gtagcggtga
     7201 tgaccgccgg cgcggcgaag cgctcgaccg cccagccagc atagcgcacc ttgcgcccgg
     7261 cgatggggtc gccggtatac tggccccagt cggcggccgt gtctgtgtca gcgacgggcc
     7321 ggccggtggc ctcgtcacgt cgccggaaga ggatttgccc ctcggcggcg aaaaggccgg
     7381 ccacacggta gggggccagc gacgggccag tccagccgcc gcggcccgtg tcgccatcga
     7441 gatagaccag cacgtcgacc ggctgcccgg tggcatcggc cagcacgacc tcgtcaccgc
     7501 tgttgctata gccgggccag gcggccagtt gcagatcagc gtcgtggcca aactgcgcgc
     7561 ggaaggcggc ggcatcgcgg gccagccaca gcgtcgcccc cggcgccagc gacgtgccgc
     7621 cggggatgag cgccacgtcc gcgccgtcgc ccagccgcca gccgctcaga tcggccggcg
     7681 ccgcgccggc gttgaccagg cgcacagcct cgtcggcgtc gtcaagggcg tagccgtcaa
     7741 agaggacact atcgatgagc acagccggcg cggccaccgg ggcggcagcc aaacggcccc
     7801 agcgggcggc gaccagcgcg acgggaatga gaacgagcaa gagcgagagg gggcgatgcg
     7861 tgcgggccat gattttgcca ttgggatgat gctatcgctg gcgcggcgcg aagggcatgg
     7921 ctcagcggcg cagcagccgg gcatagagca cgcccggcga acagcgaatg gcgatgtgcc
     7981 ggccgtggcg cacgctgggc cggtcaagtt cggcctccgt gaagtcgtcg gctgctgcca
     8041 gccactctgt ccgtccattg acctcaatgg ggtaaggctt gccccaatcg gctgtgccgc
     8101 gcatgaagtt atacgcccgc cgcgccggcc agccggccga cagggcgaag tccgcgtccg
     8161 ccggcgtggg ttggtagctg cctggcccct gcggccgccg ccggaccacg cccgcggcca
     8221 ggtcgtcgag cacaccgcgc agcagcgcca gtccggccag cgccagccgc tgctccgcct
     8281 ccgggccggg gaggccgtcg ggcaggggca cggccgtctg cgcggcgatg tcgcccgtgt
     8341 ccagccccgt gtcgagatag tggacggtcg cgccggtcgg cgcgccctcg cgcagctgcc
     8401 agaagagcgg ctgcgggcca cggtaagcgg gcagcagcga cggatgcagg ttgaggaagc
     8461 cgcgcggcgg caaggccagc agcgccggcg ggacgcgccg ggtaaagcag gccatacacg
     8521 caacatcagc gcgcgccgcg gccaacgcgg ccagcgtgcc ggcgtggccc agcgcgcgag
     8581 cggcgaagac gggcagcccg gcggcccagg cgcaaccaag cagaccggcc ggcgcgtcgc
     8641 ccagggccag cgccagcggc gcggcggccg gcggcgcgag gcgggcgatg ggcggaacgg
     8701 gatcgggcaa caggtggggc acagacgcgg cgggcacaac cacgccagcc acggcgcggc
     8761 catcagccag caacccgcgc aagaccacgg ccgacacccc cgccggcagg ccgaagaaga
     8821 ggatgcgcat gaacggcatt ataagcctgt cggggataga aggataggct ggcccgccgg
     8881 ccggtttgcg acgggcgggc gaaaggttta tcataggctc cgcggctggg gcgcgactgt
     8941 gcgccgcccg cggcgaggga tctctatgac acgcgagacg aagacattga ccgtggtgca
     9001 gtgcgccaag ctgggcgcgg aattgccggc gctcgaccgg ccgcccttcc ccggcaagct
     9061 gggccagcgc atctacgacg agatatccaa gtacgcctac agcctgtggc aagaccaggc
     9121 ccgactgctc atcaaccact atggcctgaa catggccgac ccgcgctcac aggagttcct
     9181 ctttgaacag atggaggcgt ttctgttcga cgagggatcg ccggccgtca gcggcgcgcc
     9241 ggtggccggc ggcaagggcg gcccggcccg caagtaagcc tcatgagcgc cgcgcccggc
     9301 gacgtcctgc ttctggcctg ctacgagtta ggccaccagc cgctgagcct ggcctggccg
     9361 gcggcggtcc tgcggcaagc gggcctacca gtcacggtcg ccgatctggc tgtggacgcc
     9421 ctgccgcgcg aggccgttgt ccgtgcccgc ttcgttggca tcagcgtgcc catgcacacc
     9481 gccctgcgtc ttggcgtccg ggccgcggcc gaagtgcgcg ccctcaaccc caccactcat
     9541 atctgcttct atggcctcta cgcctggctc aacgccgact acctcttggc caccgccgcc
     9601 gatgcggtgc tggccggcga actggaggag gagttggttg tccaggcgcg gcgcgtgctg
     9661 gacgcgaagc cggcggaaga caacgcgcgc gccgaccgcc gcctgacccg tctggccctg
     9721 cccacgcccg accgcgccgg gctgccgccg ctgagccact acgcccgcta tgtcgagggg
     9781 ggcgtggccc ggctggcggg caccgtcgag gccagccgcg gctgcctcca cacctgccgc
     9841 cactgcccgg ttgtgcccgt ctatggcggg cgcttcttcg tcgtcccggc cgagacggtg
     9901 ctggccgaca tccgccagca ggtcgccgcc ggcgcggaac atatcgcctt cggcgacccc
     9961 gatttcctca atggccccgg ccacgtgctg aagatcgccc gcgccctgca cgccgaattc
    10021 ccgcgcctga cgttcgactt caccaccaag gtggagcaca tccgccagca ccgcgctctt
    10081 ttcccggagt tgcgggcgct gggggccagt ttcgtcgtct ccgccttcga gagcgtctcg
    10141 gacgaagtgc tggcgcgcct gcataagggc cacaccgccg ccgatctcga cgacgccctg
    10201 ggcgtgctgg ccggtgcggg cctggccgtc caacccacgt ggctgccctt cacgccgtgg
    10261 acgacgctcg acgact
//
LOCUS       DAOSMQ010000047        10269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6517_length_10269_cov_5.332974, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000047 DAOSMQ010000000
VERSION     DAOSMQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6517_length_10269_cov_5.332974"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /locus_tag="PK829_02790"
     CDS             complement(<1..1855)
                     /locus_tag="PK829_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU40173.1"
                     /translation="MKLHKLSLQGYKTFAARTEFVFDDGVTAIVGPNGSGKSNIADAL
                     RWVLGEQSYSTLRARRTADMIFAGSQQRTRAGMAQVTITLDNSDGWLPIDFSEVEITR
                     RAFRSGENEYLINGRHVRLKDVADLLATSGLAERTYTIVGQGLIDQALSLRSDERRAL
                     FEEAAGITHYKTRRAETLRRLEETQRNLQRVRDILEELRPRVASLRRQATRTRNYEQI
                     SADLRELLRVWYGYKWEQAKVELRAARRAAETATAGWTTARDALLAHQNRIDDIQRRL
                     TATQRAAADRQTARDRLRQETEAGRRRRAVARERRAALERQLAEAEAERPLLEEQAAA
                     AHAAIAAAATDLLAAQTEMQGQTARLTEFRVAFEGQRAEVNRRQAEVRRLEGELRQTQ
                     TAQAQAEGQLAQLHERLAEQSVAPPAEPSPADPAALAHLEAVATEAQAAVAQQRAARE
                     ETERARVATLARLKELRRAAREADNDLNRRRGDLARAEERLALLDQQRQRAIPLDAAR
                     VVGQLAGLLRIPPAHERAIAAALGEQLAAWLVSDVESLWQAVESARDGDAGARLSLLV
                     ADTPSAGLALDQSPDQRPTVADTRGVIGWAVERVAFDIAAAPLAGQLLGRLL"
     gene            1968..4115
                     /locus_tag="PK829_02795"
     CDS             1968..4115
                     /locus_tag="PK829_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU40174.1"
                     /translation="MAQANVNRQALVEAIATLESQRRSDEPTLDRAVATALSALRDKL
                     ADLQSNPGDQQQRHLAVLMADLSGFTALSERMDAEKVRDALNAMWRVLDAVILAHGGQ
                     IDQHAGDSLLALFGLPQPRAGDAARALRAALTLQAELALFNERVRQAAASQGAPWAAD
                     WPGPAMRVGVHSGPVYFARAAGSARLTSVGETIAVARRLEHVAPPGGVLASATAQHQA
                     AAQVQFADAPAPLAAAAGRPTGEALYLATGERPDFPSFGPALTAGQVTRLIGRDEQLD
                     RLENALQAVIDSGAPQVLTMIGAAGSGKSRLVHEFEARARLLTGVSGVQPTGGRLFAA
                     EAPFALVRDLLLRRFGLRPQHSHAIVEDRIRRVLDTLDRPGRARRLSATGPLDSTLEL
                     LKQLLNVQTAATLPPEEVDALVERLLGAASSAGPTIVVLEDAHRADRQSLDLVDRLAR
                     AGGPLPVLFLVLAAQDGDTPPALPWLGREEDPFLPFDRLEVTPLSAVDSRLMATDILG
                     QLSPPMRLLDLIVAESGGNPLYIEAFIRLLLERGVISVGERRGFDMAQLERMRLPAGL
                     PGLIETRMQALTDAERHVLQAAAVAGPLFWDVTLLDTRSPLSDDLTEPEIEAALLSLV
                     SKRFILPDTTYSFGAAQAYRFRREVGYAVAYDSLPPAARLAQHARVAQWLLANQTDAR
                     FRAWFPVDGMIAYHLAAGGEATRAFNWRQRAGQ"
     gene            4122..7301
                     /locus_tag="PK829_02800"
     CDS             4122..7301
                     /locus_tag="PK829_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU40175.1"
                     /translation="MVSRDQLQEAIAQFTDQRARFGAATETILAVLRERLAALPSEPT
                     AATKPPQPAGEQRKQVTVLFAAIDGFTRLAGAIHNTERLRQIDLLWRQLDETIYAHGG
                     IVDKHMGDVVMGIFGVPTARENDPERAVRCAIAMRELAGEALAALGGKGVSDKGRELV
                     IRIGINTGPVSLGQVGSDDGQTAIGDAVNVASRLKETADEAGVYISQATYRLVHRLFR
                     VETLGPVAIKGRQMPVTAHRVVGSRPRAFFPESDGLEGVAVPMIGRDAELTLLLRALR
                     DTIETGQGRLITIVGEAGVGKSRLVREFSRRLEGLPVKMALFQGRTDQRLMEVPYGLL
                     RDLVVDHFHIEEGDRGRQIEDKLARRLAEVLGRSRGARRRTSEGGAGRSGAAERARQI
                     AHLVGLEMAAPDADLPAPQRAAARERAVETLLAYFVAVARRSAATLFILEDVHWADDD
                     SLALLEQLSALTADAPLMMICLARPLLFERRPVWAAESATPATLPLAPLSENHSRELV
                     LQILRKLPDIPAALLDLIVQSAAGNPFYVEELVRVLIEDGVIVPGETAWQLRRRELTH
                     LRVPATLTGVLQARLDRLPELERLTLQQAAVVGDEFWDSAVQQINSAARNPFDGEQVA
                     AALQSLERRDMIYRVPSPVFNGSQAYLFKHAVLREVAYESVLLRDRPGYHLQAARWLE
                     AQSGERLADYAAPIAQHHERAGLPAEAARLYDLAARRAAEQYKPALAIDYYRKALGLL
                     ENLPQFLDLRLNVQEQMGRVLRRQGRLVEALYTYHAMQRLAELDGNLQGEARAENALA
                     GVFADLNDFNAALAAASRAERLARLTGADIEWARALLWRAEAAGQLGHADEARAAATE
                     AADRARALDAPRELARALALLLALAEPAQAATIAAELAALADALEARGASEDAAAALT
                     LLGEAHLRQGDATAALGALERALAARRALGAPGNAADTLRLLGLAVCRAGQVEAAVDY
                     LEEAGALAETAGQRYLRLLCRLALAEALLAHGRAEAAEATLRGVIAAAEDGQRLGTWR
                     HLPEAYDLLALVLQRQGRGDEAVWRTARR"
     gene            7501..8322
                     /gene="cdaA"
                     /locus_tag="PK829_02805"
     CDS             7501..8322
                     /gene="cdaA"
                     /locus_tag="PK829_02805"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOU40176.1"
                     /translation="MELFNSLLERLALIRVVDVIDILLVTLIIFGVLMLIRGTRAVQV
                     LRGMLVLATIIFVLAQIFELRGFTWLLDNMLPVLLLAIPVIFQPELRRALEQLGLAGR
                     YLRFFRHDEINPVVVTFKDAALRLSQRRHGALVVFEQDTGLQEYIDTGVKLEAEPSPE
                     LFLTIFNKHTELHDGAIIIRGEKLAAAACVLPLSTSSLSDRQMGLRHRAALGISEVSD
                     AVAVVVSEETGQVSIAHNGRILRRQEIARLDAILHALLTSPRQPISGKDSREPRA"
     gene            8306..9673
                     /locus_tag="PK829_02810"
     CDS             8306..9673
                     /locus_tag="PK829_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOU40177.1"
                     /translation="MNRARDLLTNVASFLLALVLAIAIWTNASEAQDPVLTRFLEVPL
                     NIVGLPADTVLAGADVRQAVQIRLEGPDSILEATSPADFTATADVSQVPPGETTSVDI
                     TVTNRRPGATISFITPESIDVLLEQQVTRDVPVELDLRGTVARGHNQGEPLLDPPTIR
                     VSGAASRVNDLDFALVTVFLNSTVETLVDTSEPIFYDRSGRVASVTGLDLSSQEVTVT
                     IPVEEAAGFADKLVTAVWSGEPAPGYRLLSITVDPPSVLVEGRPALVNRMTSARTEAI
                     DITGLTQTFKQAVILDLPEGVTVDPEQTVTVRIEIEPILTTSTFIRVPETRGLPSTLE
                     ATATPSQVRVVLFGPLPVLDALAENDVRVTLDLFGLEPGTYSIEPDVSVPDRDIEIRS
                     VQPSAVSVTITEPQAGAEATPTRVAARATTQRVASLPAVCFLPPNGAVDEALTTFCAG
                     RQSSQ"
     gene            9670..>10269
                     /locus_tag="PK829_02815"
     CDS             9670..>10269
                     /locus_tag="PK829_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOU40178.1"
                     /translation="MNQKSLVALVGRPNVGKSTLFNRIIGRRLAVVSDVAGTTRDRLY
                     ADAEWGGVVFTLVDTGGIEVTEGRGTAPLSEDSEQFLPLIRQQATVAMQDADAIVHVV
                     DGQSGITAADREVADILRQTKKPVFVAANKLESSKLWDTAYEFYELGLGEVFAVSALH
                     GSGTGDLLDAIVGAVSPFDPEEEAEDTSVKIAILGRPNVG"
BASE COUNT         1468 a         3658 c         3502 g         1641 t
ORIGIN      
        1 gcagcagccg gccgagtagc tgcccggcca gcggcgcggc ggcgatgtcg aaggcaacgc
       61 gctccacggc ccagccgatg acgccgcgcg tgtcggccac ggtcgggcgt tggtcgggcg
      121 actggtcgag ggctaaaccg gcggacggcg tgtcggcgac cagcaggctc aggcgcgcgc
      181 cggcgtcgcc atcgcgggcg ctctccacag cctgccacag gctctcgacg tcactcacca
      241 accaggcggc caattgctcg cccagcgcgg cggcgatggc ccgctcgtgg gctggcggga
      301 tgcgcagcaa cccggccagc tgccccacca cgcgggccgc atcgagagga atggcccgct
      361 gccgctgctg gtcgagcagg gccaggcgct cctcggcgcg ggccaggtca ccgcggcggc
      421 gattgaggtc gttgtcggcc tcgcgggcgg cgcggcgcaa ctccttcagg cgagccagcg
      481 tggcaacgcg ggcgcgctct gtctcctcgc gggcggcgcg ctgttgggcc acagccgcct
      541 gggcctcggt ggcgacagcc tccaggtggg ccagcgccgc cggatcggcc ggcgacggct
      601 cggccggtgg cgcaaccgac tgttcggcca ggcgctcgtg caactgggcc agctgcccct
      661 cagcctgggc ctgggccgtc tgtgtttgcc gcagctcgcc ttccagccgt cgcacctctg
      721 cctgccgccg gttgacctca gcacgctgcc cttcaaaggc aacgcggaac tcggtcaggc
      781 gggccgtctg accctgcatc tccgtctgcg ccgccagcag gtcggtcgcg gcggcggcga
      841 tggccgcgtg ggcggcagcg gcctgctcct ccagcagcgg tcgctccgct tccgcctcgg
      901 ccagttgtcg ctccagtgcc gcgcgccgct cgcgggccac ggcccggcgg cggcggccgg
      961 cctcggtctc ctgccgcaga cggtcgcgcg ccgtctggcg atcagccgcg gcccgctgcg
     1021 ttgccgtcag gcggcgctgg atgtcgtcga tgcggttctg gtgggccagc agcgcgtcgc
     1081 gcgccgtcgt ccagccagcc gtagccgtct cggcggcgcg gcgggcggcg cgtagctcca
     1141 ccttagcctg ctcccattta tagccgtacc agacgcgcaa caactcgcgc aaatcggccg
     1201 aaatctgctc gtagttgcgc gtgcgcgtgg cctgccgccg cagcgaggcc acgcgcgggc
     1261 gcaattcctc caggatgtcg cggacgcgct ggaggttgcg ctgcgtctct tccagccggc
     1321 gcaatgtctc ggcccgccgg gttttgtaat gggtgatgcc cgcggcctct tcaaacagcg
     1381 cccggcgctc gtcggatcgc agcgacagcg cctggtcgat gaggccctgg ccgacgatgg
     1441 tgtaggtgcg ctcggccagg ccgctggtgg ccagcaggtc ggccacatcc ttcaggcgca
     1501 cgtgccggcc gttgatgagg tactcgttct cgccagagcg aaaggcgcgg cgggtgatct
     1561 cgacctcgct aaagtcgatg ggcaaccagc catcgctgtt gtccagggta atggtcacct
     1621 gggccatgcc ggcgcgcgtc cgctgctggc tgccggcgaa gatcatgtcg gccgtgcggc
     1681 gggcgcgcag cgtgctgtag ctctgctcgc ccagcaccca gcgcaacgca tcggcaatgt
     1741 tgcttttgcc gctgccgttc ggcccaacga tggcggtcac gccatcgtcg aagacgaatt
     1801 ccgtccgcgc ggcgaacgtc ttgtatcctt gcaggctgag cttgtgcagc ttcatggcta
     1861 tcgcagtcgg ggacgcccgc cgacttgtga cactccctct tcgcccggtc ggtcgcccgt
     1921 tccgggccgg ttcgcgtgcg ggcaaggcta tttatataat acctggtgtg gcccaggcaa
     1981 acgttaaccg ccaagcgtta gtggaggcca tcgccacgct cgagtcgcag cggcgatcgg
     2041 acgaaccgac gctcgaccgg gcagtggcca cagcgctttc ggccctccgc gacaagctgg
     2101 cggacctgca atcgaatcct ggcgaccagc agcagcgcca cctggcggtg ctgatggccg
     2161 atctttccgg ttttacggcg ctgtcggagc gcatggacgc cgagaaggtg cgcgacgcgc
     2221 tcaatgccat gtggcgggtg ctcgatgccg tcattctggc ccacggcggc cagatcgacc
     2281 aacacgccgg cgacagcctg ctggcccttt tcggcctgcc gcagccacgc gccggcgacg
     2341 cggcccgcgc cctgcgcgcg gcgctgacgt tgcaggccga actggcgctg ttcaacgaac
     2401 gtgtgcgcca ggccgccgcg agccagggcg cgccctgggc ggccgactgg cctggcccgg
     2461 ccatgcgcgt cggcgtccac agcggcccgg tctacttcgc ccgcgccgcc ggcagcgctc
     2521 ggctgacgtc tgttggcgag actatcgccg tggctcgccg gctggagcac gtcgcgccac
     2581 ccggtggggt gctggcgtcg gccaccgccc agcatcaggc ggccgcgcag gtgcaattcg
     2641 ccgacgcgcc cgcgcccctg gccgccgcgg ccggccggcc gacgggcgaa gcgctctacc
     2701 tggccacggg cgagcgcccc gacttcccat ccttcggccc cgcgctgacg gccggccaag
     2761 tgacgcgcct gatcggccgc gacgagcaac tcgaccggtt ggagaacgcc ttgcaggcgg
     2821 tgatcgacag cggcgcgccc caggtattga ccatgattgg cgcggccggt tcgggcaagt
     2881 cgcgcctggt gcacgagttc gaggcgcggg cgcggctgct aaccggtgtg tcgggcgtcc
     2941 agcccaccgg cggacgcctg ttcgccgccg aagcgccctt tgccctggtg cgcgacctgt
     3001 tgctgcgccg cttcggcctc cgcccccagc acagccacgc catcgtcgaa gatcgtatcc
     3061 gccgcgtcct cgatacgctc gaccggcccg gccgcgcccg ccgcctgtcg gcgaccggcc
     3121 cgctcgactc gacgctggaa ctgctgaagc agctgctgaa cgtgcagacc gccgccaccc
     3181 tgcccccaga ggaggtggac gcgctggtgg agcgtcttct gggggccgcc agctcggccg
     3241 gcccgaccat cgttgtcctg gaggacgccc accgcgccga ccgccaatcg ctcgacctgg
     3301 tagaccggct cgcgcgggcg ggcgggccgc tgccggtgct gttcctcgtg ctggccgccc
     3361 aggacggcga cacgccgccc gctctgccct ggctggggcg cgaggaggac ccgtttttgc
     3421 cgttcgaccg gctggaggtc acgcccctgt cggctgtcga tagccgcctg atggccaccg
     3481 acatcctcgg ccagctctcg ccgccgatgc gcctgctcga tctcatcgtc gccgagtccg
     3541 gtggcaatcc gctctacatc gaggcgttca tccggctgtt gctggaacgt ggcgtcatct
     3601 ccgtgggcga gcggcgtggc ttcgacatgg cccaactgga gcggatgcgg ctgccggccg
     3661 gtctccctgg gctaatcgag acgcggatgc aagctctgac cgacgccgag cggcacgtct
     3721 tgcaggccgc cgccgtcgcc gggccgctgt tctgggatgt caccctgttg gacacccgct
     3781 cgcccctgtc ggacgacctg accgagccag agatcgaagc cgccctgctg agcctggtca
     3841 gcaagcgctt catcctgccc gacacgacct acagcttcgg cgcggcccaa gcctatcgct
     3901 ttcggcgcga ggtgggctat gcggtggcct atgacagtct gccgcccgcg gcgcggctgg
     3961 cccagcacgc ccgtgtggcc caatggctgt tggccaacca gaccgacgcc cgcttccggg
     4021 cctggttccc ggtggacggg atgatcgcct accacctggc cgccggcggt gaggcaacgc
     4081 gggccttcaa ctggcgacag cgcgccgggc agtaacaccc catggttagc cgcgaccaac
     4141 tccaggaagc catcgcccag ttcaccgacc agcgcgcgcg cttcggcgcg gcaacggaga
     4201 ccattctggc cgtcctgcgg gagcggctgg cggcgctgcc gagcgagcca acggccgcaa
     4261 ccaagccgcc acaaccggcc ggcgagcagc gcaagcaggt caccgtgctc ttcgcggcca
     4321 tcgacggctt cacgcgtctg gccggagcca tccacaacac cgagcggctg cggcagatcg
     4381 acctgctgtg gcggcaactg gacgagacca tctacgccca cggcggcatt gttgacaagc
     4441 acatgggcga cgtggtcatg ggcatcttcg gcgtgcccac ggcgcgcgag aacgacccgg
     4501 aacgcgccgt ccgctgcgcc atcgccatgc gcgagttggc cggcgaggcg ttggcggcgt
     4561 tgggcggcaa aggcgtgagc gacaagggac gcgaactggt catccgcatc ggcatcaaca
     4621 ccgggccggt gtcactgggg caggttggct ccgacgatgg ccagacggcc attggcgacg
     4681 ccgtcaacgt cgccagccgc ctgaaggaga ccgccgacga ggctggcgtc tatatctccc
     4741 aggccaccta ccggctggtg caccgcctct ttcgcgtgga gacgctgggg ccggtggcca
     4801 tcaagggtcg ccagatgccg gtgacggccc atcgcgtcgt cggctcgcgg ccgcgcgctt
     4861 tcttccccga aagcgacggg ctggaaggtg tggccgtgcc gatgatcggc cgggatgccg
     4921 agctgaccct gctgctgagg gccttgcgcg ataccattga gaccggccag gggcggctga
     4981 tcaccatcgt cggcgaggcg ggcgtgggta aatcacgtct ggtgcgtgag ttcagtcgcc
     5041 ggctagaggg cctgcctgtc aagatggccc tgttccaggg gcgcaccgac cagcggctga
     5101 tggaagtgcc ctacggtctg ctgcgtgatc ttgtcgttga tcacttccac attgaggagg
     5161 gcgaccgcgg ccggcaaatt gaggacaagc tggcccgccg cctggccgaa gtgctgggtc
     5221 gcagccgggg cgcgcgccgt cgcacgagcg agggcggcgc gggccgcagt ggcgcagccg
     5281 agcgggcacg gcagatcgcc cacctggtcg gcctggagat ggccgcgccc gacgccgatt
     5341 tgcccgcccc acaacgcgcc gcggcgcgcg agcgcgccgt cgagacgctg ctggcctact
     5401 tcgttgccgt cgcccggcgt tcggcggcaa cgctgtttat cctggaggat gtccactggg
     5461 ccgacgacga ttcgctggcc ctgctggagc agttgtcggc cctgacggcc gacgcgccgc
     5521 tgatgatgat ctgtctggcc cggccgctgc tgttcgagcg ccgtcccgtc tgggccgccg
     5581 aaagcgccac cccggccacc ctgcccctcg ctccactgag cgagaaccac agccgtgaac
     5641 tggtgttgca gattttgcgc aagctgcccg acatccccgc ggcgctgctg gacctcattg
     5701 tccaatcggc cgcgggcaac cccttctacg tcgaggagtt ggtgcgcgtg ctcatcgagg
     5761 atggcgtcat cgttcccggc gagacggcct ggcaattgcg gcggcgcgag ctgacccatt
     5821 tgcgcgtccc ggccacgctg acgggcgtgt tgcaggcccg cctcgaccgg ctgccggagc
     5881 tggaacggct aacgttgcag caggcggctg tcgtcggcga tgagttctgg gatagcgccg
     5941 tgcagcagat caacagcgcg gcgcgcaacc ccttcgacgg ggagcaggtc gccgccgcgc
     6001 tccagtcgct ggagcggcgc gacatgatct accgcgtacc ctcgcccgtc ttcaacggca
     6061 gccaggccta cctgtttaag cacgctgtcc tgcgcgaggt ggcttacgag agcgtgctgc
     6121 tgcgcgaccg gccgggttac cacttgcagg cggcgcgttg gctggaggca caaagcggcg
     6181 agcggctggc cgactatgcc gcgcccatcg cccagcacca cgagcgcgcc ggcctgccgg
     6241 ccgaggcggc gcgcctctac gacctggccg cgcggcgggc agccgagcaa tacaagccgg
     6301 ctctggccat cgattattac cgcaaggcgc tggggctgct ggaaaacttg cctcagttcc
     6361 tcgacctgcg cctgaacgtg caggagcaga tgggccgcgt cctgcgccgg caggggcggc
     6421 tggtggaggc gctctacacc taccacgcca tgcaacggct ggccgagctg gacggcaact
     6481 tgcagggcga agcccgcgcc gagaacgcgc tggccggcgt ctttgccgat ctcaacgatt
     6541 tcaacgccgc gctggccgcc gccagccgcg ccgagcgact ggcccgcctg accggggccg
     6601 acatcgaatg ggcgcgggcg ttgctgtggc gggccgaggc ggccgggcag ctgggtcacg
     6661 ccgacgaagc gcgcgcagcg gcaaccgagg cggccgaccg cgcccgcgcc ctcgacgcgc
     6721 cgcgtgaact ggcccgcgcg ctggccttgc tgctggctct ggccgagccg gcccaggccg
     6781 ccaccatcgc cgccgaactg gctgccctgg ccgacgctct ggaagcgcgc ggcgcgagcg
     6841 aggacgccgc cgcggcgctg acgttgttgg gcgaggccca tctacggcag ggcgatgcca
     6901 ccgcggcgct gggcgcgctg gagcgcgcgc tggccgcccg gcgcgcgctg ggcgcgcccg
     6961 gcaacgccgc cgatacgctg cggctattgg gcctggccgt ctgtcgcgct ggccaggtgg
     7021 aggctgccgt ggactattta gaggaggccg gcgcgctggc tgaaacggcc ggccaacgct
     7081 acctgcggct gctgtgccgg ctggcgctgg ccgaggcgct gctggcccac ggccgcgccg
     7141 aagcggccga ggcgaccctg cgcggcgtca tcgccgccgc cgaggacggg cagcggctgg
     7201 gcacatggcg gcatctgccg gaggcctatg acctgctggc cctcgtcttg cagcgccagg
     7261 ggcgcggcga tgaggccgtt tggcgtaccg cgcgtcgctg aaccgtccgc gtcagtggtg
     7321 atctggttgc cctgtggcct cttcgcgccg gccaatcgtg gctttcccgt ctctttggct
     7381 gtggtatact tgttcaccgg gtttgtgcac ggccagcgcc tacagccatc accctggcag
     7441 gtatgaccgc tttaacgaaa ttgatggttc ccggggccgg gaatcgtttc gagcacacta
     7501 ttggaactct tcaactcatt actggaacgc ctcgccctca tccgcgtcgt cgatgtcatc
     7561 gacatcctgc ttgtcaccct gatcatcttc ggcgtgctga tgctcatccg cggcacgcgc
     7621 gccgtgcagg tgctgcgcgg catgctggtg ctggcaacta tcatcttcgt cctggcccag
     7681 atcttcgagt tgcgcggctt cacctggctg ctggacaata tgctgcccgt gctgctattg
     7741 gccatcccgg tcatcttcca gccggaactg cggcgagcgc tggagcaatt ggggttggct
     7801 ggtcgctatc tgcgcttttt ccgccacgac gagatcaacc ccgtcgtcgt caccttcaaa
     7861 gacgcggccc tgcgcctctc gcagcggcgg cacggggcgc tcgttgtctt cgagcaagac
     7921 accggcctgc aggaatacat cgacacgggc gtcaagctgg aggccgagcc gtcgccagag
     7981 ctattcctga ccatcttcaa taagcacacc gaactccacg acggggcgat catcatccgt
     8041 ggcgaaaagc tggccgcggc cgcttgcgtc ctgcccctgt ccaccagcag cctgtccgac
     8101 cggcagatgg ggctgcgcca ccgcgccgct ctgggcatca gcgaggtcag cgacgccgtg
     8161 gctgtcgtcg tctccgagga aactggccag gtgtccatcg cccacaacgg ccgcatcctg
     8221 cgccgtcagg agatcgcccg cctggacgcc atcctccacg ccctgctgac cagcccccga
     8281 cagcccattt cggggaagga ctctcgtgaa ccgcgcgcgt gatctcctga ctaacgtcgc
     8341 ctccttcctg ctggcgcttg tcctggccat cgctatctgg actaacgcct ccgaggccca
     8401 ggacccggtg ctgacgcgct tcctggaagt gccgttgaac atcgttggtc tgccggccga
     8461 caccgttctg gccggggccg atgtgcgcca ggcggtgcag attcgcctgg aagggccgga
     8521 ctccatcctg gaggcgacct caccggcgga cttcaccgcc acggccgacg tcagccaggt
     8581 tccgcccggc gaaacgacgt cggtcgatat caccgtcacc aatcgccggc cgggcgcgac
     8641 catctccttc atcacgccgg agtccatcga cgtcttgctg gaacagcagg tgacgcgcga
     8701 cgtgcccgta gagctagacc tgcgcggcac agtcgcccgc ggccacaacc agggcgagcc
     8761 gctgctggac ccgcccacca tccgcgtgtc cggcgcggcc agccgcgtca acgacctcga
     8821 ctttgccctg gtgacggtct ttctcaatag cactgtcgag acgctggtag acacatccga
     8881 accgatcttc tacgaccgct ccggccgcgt tgccagcgtc actgggcttg acctgagcag
     8941 tcaggaagtg acagtgacta tccccgtcga ggaagcagcc ggctttgccg acaagctggt
     9001 gacagcggtc tggtcgggcg agccggcccc cggctaccgc ttgctcagta ttacggtcga
     9061 tccgcccagc gtcctcgtgg agggccgtcc ggcgctggtc aaccgcatga ccagcgcgcg
     9121 cacggaggcc atcgacatca ctggactgac gcagacgttc aagcaggccg tgatcctcga
     9181 cctgccggag ggcgtcaccg tcgacccgga gcagaccgtc accgtccgca tcgaaatcga
     9241 acccatcctg accaccagca ccttcatccg cgtgccggag acgcgcgggc tgccgtccac
     9301 cctggaggcg acggcgacgc cctcgcaggt gcgggtggtg ctctttggcc ccttgcccgt
     9361 gctcgacgcg ctggcggaga acgacgtgcg cgtcactctc gacctgttcg gcctggaacc
     9421 gggaacctac tctatcgaac cagatgtcag cgtgcccgac cgggacatcg agattcgttc
     9481 cgtgcagcct tccgctgtta gcgtgacgat cacggagccg caggccggag ccgaggccac
     9541 gccgacgcgc gtggcggcgc gggccacgac gcagcgggtg gccagcctgc cagccgtctg
     9601 ctttctgccg cccaacgggg ccgtggatga ggccctgaca acgttctgcg ccgggaggca
     9661 atcatcgcaa tgaaccaaaa atctctggtg gccctggtgg gccggcccaa cgtgggcaaa
     9721 tcgaccctgt tcaaccgcat catcggccgt cgcctggccg ttgtctccga cgtggccggc
     9781 accacccgcg accggctcta cgccgacgct gagtggggcg gtgtggtctt cacgctggtc
     9841 gataccggcg gcattgaggt caccgagggc cggggcaccg cgcccctgtc cgaggattcg
     9901 gagcaattcc tgcccctcat ccggcagcag gccactgtgg ctatgcagga cgccgacgcc
     9961 atcgtccacg tcgtcgatgg ccaatcgggc atcaccgccg ccgaccgcga ggtggccgac
    10021 atcctgcgcc aaaccaagaa gcccgttttt gtggccgcca acaagctgga gtcgtcgaaa
    10081 ctatgggaca cggcctacga gttctatgag ttggggctgg gcgaggtctt tgccgtgtcg
    10141 gccctgcacg gctccggcac gggcgatctg ctggacgcca tcgtcggggc cgtgtcgccg
    10201 ttcgaccccg aggaggaggc cgaggacaca tcggtcaaga tcgccatcct gggccggccc
    10261 aacgtcggc
//
LOCUS       DAOSMQ010000048        10264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6524_length_10264_cov_4.374767, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000048 DAOSMQ010000000
VERSION     DAOSMQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_10264_cov_4.374767"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PK829_02820"
     CDS             <1..719
                     /locus_tag="PK829_02820"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOU40179.1"
                     /translation="LDDDQRRALHEAATAAAAGRAPVLLHVGAQRTAAAVALAQHAAT
                     LRPAALVAMTPTFYSMSDDALARYFHAIAAAAPDVPLFLYDIPQFAVNGIGPALLARL
                     AGEMPSLAGLKTSRVDIQMVRQLIDALPRDRIVLAGNESAALASLALGADGLISGLST
                     AIPEPFVALTRAAAAGQLDEAREWQRCINRLLGVLGGARIGGIKAILVQRGIPVGPPV
                     PVLDSTDAPLWTRAAEILGI"
     gene            767..1465
                     /locus_tag="PK829_02825"
     CDS             767..1465
                     /locus_tag="PK829_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOU40180.1"
                     /translation="MRLILFDIDGTLIHSDRIGRAAMARALTDVFGHCGAVDTISFGG
                     KTDCGMVHELMLAEGWSEGEVGARLPHFYERMAAAGQALFTSTHIRPCPGVLPLLAAL
                     AARADAVPALLTGNIQATAPIKLAAAGIDPGLFRVGAYGSDSAIRADLFGIALARAEA
                     ELGLRIDAHDVIIVGDTPGDIDCARAGGGRVIAVATGPYPAATLQEHRPDYLFGDLTA
                     TDAVLRALFDDHQV"
     gene            1503..1811
                     /locus_tag="PK829_02830"
     CDS             1503..1811
                     /locus_tag="PK829_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40181.1"
                     /translation="MIDDIAFNIGEPTPIPLERLYTWVVWQFPRLRSVTLGGNSTFGD
                     ADGVEGDVFTGHSGAVHPGDGEHGWYPALIDAAAGTVLIFANVAEPSPSPEAASKYFD
                     "
     gene            complement(1887..3146)
                     /locus_tag="PK829_02835"
     CDS             complement(1887..3146)
                     /locus_tag="PK829_02835"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOU40182.1"
                     /translation="MPYMEDVLGLILGGGQGTRLYPLTKERSKPAVPLAGKYRLIDIP
                     MSNCFHADIEKIAILTQFNSASLHRHIWSTYTRDQFSPGWVQILAAEQTPRSADWYQG
                     TADAVRKQWMEIKQAGAKYVLILAGDHLYRMDYRQFVGQHVESGADISVAVQPVSRET
                     VSGLGILKMDSNNVITAFAEKPKKPEVLQDFENPTNPDKPFLASMGIYVFNTDVLQAV
                     LEEEYVDFGRHILPQSIKKRRVCGYVFDGYWEDIGTIRRFFEVNLQMAKIDAPFDFYD
                     AQAPIYTHARFLPGSELHNTRLDEVLLADGCRLYDASVRECVVGLRAIIGQEASIQSS
                     IVMGADYYETEDDRAENRRRKRPDIGIGANAVIKGAIIDKNARLGSNVTIRYIPDRPD
                     EEGDCYVARDGIVIVPKNAVVPDGTVI"
     gene            3329..3763
                     /locus_tag="PK829_02840"
     CDS             3329..3763
                     /locus_tag="PK829_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40183.1"
                     /translation="MSRVIHVDNPGKRRNAARRSIAEILRYLSRKGQIDDEARDMAAA
                     LVFLLTEIKATVDESAAAWEKRGYWMKVERFVRDYEWIPGAAANLDDVIRHDAWDLLP
                     ELLADLSPHFDDIHIKTLTRKPTEWRGAYSRLKTTPPVAAPY"
     gene            3810..4868
                     /locus_tag="PK829_02845"
     CDS             3810..4868
                     /locus_tag="PK829_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU40184.1"
                     /translation="MKAIIVAPILLALALLLLPACGPQPALPTRAAPALFPPTPPVLP
                     PTPTTAGATTTVNTPAPTSLPVAPVAEPPLATADAATPLPLPSGEAAAAVPTGVWPIA
                     SPTPCATPGRVEAGELASAVAGQTMRYRVYLPPCYGEDGRVYPTLYLFGGNIHDETFW
                     DRLGFDEAAEAAIVAGRIPPLLIVMPDNGWLANTTSSGPTSYEGFVLNELIPHIESQT
                     CAWPAREGRAVGGVSRGGYWSLMMAFRRPDLFRSVGAHSPALIDSFAGPDEDPRVTGT
                     ANPLGDLRIWVDIGERDPYLTAARPLHDTLTAAGVAHEWRVNPGIHDEAYWTAHLDDY
                     ITWYAAGFPADRAALPGC"
     gene            complement(4865..5965)
                     /locus_tag="PK829_02850"
     CDS             complement(4865..5965)
                     /locus_tag="PK829_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40185.1"
                     /translation="MQAEAIGLWAARALALALGLYIVAASVLAAIKTFILPRPAQVRT
                     IRLTFAAVRWLFNWRGRRAISYNDRDRIMALYAPMALFFVPIVLMILVFTGFALIYWA
                     LSPLSILESIKLSGSALFTLGSFTNDDPLLMVFEFGQALLGMIMVALLIAYLPTIYSA
                     FTRRETQVQLLEVRAGTPPSAWEMLIRSYRNGELEQLREFWNDWQVWFAELEESHTSL
                     TALAFFRSPRPDLSWVTAAGVSLDAASLTLSTLDIPWEPRAAFCIRSGYLALREIATF
                     FGVEHNSNPKPDDPISIAREEYDSVCAQLAAAGVPLLADREKAWRDFNGWRVNYDTVL
                     IALAALTMAPYAPWSSDRSVAGARPAQSRWQA"
     gene            6088..6888
                     /locus_tag="PK829_02855"
     CDS             6088..6888
                     /locus_tag="PK829_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOU40186.1"
                     /translation="MDYSDYQNILFERREHGIVWLTLNRPEALNAADRRLHTELVEVW
                     RTLDRDPDARVAVVTGAGRAFSAGGDLGMVADAYRNPEEIGRILDEARDLVYNILRCD
                     KPIVSAINGPAVGAGLVVALLADISIVAESARLGDGHVRMGVAAGDHAAIVWPLLVGM
                     ARAKYYLMTAEFIDGKEAERIGLVSLCVPDEELLERAQAVAVKLATGPRHALRYTKRA
                     LNQWLLQAGPIFDHSLALEMLGFFHEDMAEGVAALQGKRAARYPSSEG"
     gene            complement(6932..8278)
                     /locus_tag="PK829_02860"
     CDS             complement(6932..8278)
                     /locus_tag="PK829_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOU40187.1"
                     /translation="MRSLLTDSAERAIAYLEGLDARPVAPMVVDRLDELDRPLPDQPT
                     PPEEVLRDLDEIGSPATMAMAGPRFFGFVIGGALPVALAANWLAGAWDQNSALYNVTP
                     ATAQLERIALEWLVSLFGLPAGTGGGFVTGATMANFTALAAARYGLLERVGWNVANDG
                     LFGAPPITVIVGQEAHVTLLKTLSLLGLGRNRVVRVPADRQGRLRAEALPPISGPTLI
                     CAQAGNVNSGAFDPLADICAHARAGGAWLHVDGAFGLWAAAVPEMAHLTDGLAGADSW
                     AVDCHKWLNVPYDSGVAFVRDSDTLRAAMAITAEYLPTASRERNPSDFAPELSRRGRG
                     VEVWAALRALGVEGVRDLIRRTCGYARRFAAELEAAGYEVLNDVVLNQVVVAFGDAAT
                     TSRVVAGIQADGTCWCGPTVWQGRTAMRISVSSWATTDEDVTRSLAAMLRVARAVA"
     gene            8445..9467
                     /gene="paaA"
                     /locus_tag="PK829_02865"
     CDS             8445..9467
                     /gene="paaA"
                     /locus_tag="PK829_02865"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004939951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HOU40188.1"
                     /translation="MADEAYKGQVAAFEARIARGEKIEPGDWMPDAYRKQLIRMISQH
                     AHSEVVGMLPEGVWITRAPNLRRKMVLLAKVQDEAGHGQYLYHAAETLGVGRDEMIAA
                     LLDGRAKYASVFNYPTLGWADVAAIGWLVDGAAIMNQTMLAHCSYGPYSRAMVRICAE
                     EGFHKKQGQEMIIRYAAGTATQRAMAQEAIDRWWWPALMMFGPHDSASSNTPQLVRWG
                     IKTKTNDQLRQEFIDDMVPALQTLGLSVPDPEMRYDAATGHWAGREIDWDEFWRVIQG
                     DGPMNASRLDARRQAHEDGRWVREAAAAYGEKQPGSGSAGEQGRKESLLPPCAPAPLH
                     PSESTT"
     gene            9464..9994
                     /locus_tag="PK829_02870"
     CDS             9464..9994
                     /locus_tag="PK829_02870"
                     /inference="COORDINATES: protein motif:HMM:NF018002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein"
                     /protein_id="HOU40189.1"
                     /translation="MNDQWPRYEVFKQERDGGPFRNVGSVHAPDAEMALLNARDVFAR
                     RPACRCLWVVPAAAIVATPIVVNAAGDAEPDDVSGPLEPYAVCGKSSERQAMTYVDYL
                     GRVEARSAAEALRAAPALGAARIWWVFPERVISRSDPEDAPGLEAATGKLFRMSNQYH
                     TVVTMQKIRRGDEGEE"
     gene            9991..>10264
                     /locus_tag="PK829_02875"
     CDS             9991..>10264
                     /locus_tag="PK829_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HOU40190.1"
                     /translation="MIDPTLAVPLADRLLAMADDELILAHRNSEWAGHGPILEEDIAF
                     ANIALDEMGHAAIWYGLRSDITGEDTDRLVFFRNAGQYRNVQMVELP"
BASE COUNT         1728 a         3528 c         3377 g         1631 t
ORIGIN      
        1 agctagacga cgatcagcgt cgtgccctgc acgaggcggc cacggccgcc gccgccgggc
       61 gcgcgcctgt gttgcttcat gtcggcgcgc aacggaccgc ggcggccgtg gccctggccc
      121 agcacgccgc cacgctgcga cccgcggccc ttgtggccat gacgccgacc ttctacagca
      181 tgagcgacga cgctctggcc cgctatttcc acgccatcgc cgcggccgcg ccggatgtgc
      241 ccctgtttct ctacgacatc ccccagttcg ccgtcaatgg cattggcccg gcgctgctgg
      301 cgcggctggc gggtgagatg ccgtcgctgg ccggcctgaa gacgagccgc gtggacatcc
      361 agatggtccg ccaactcatc gacgccctgc cgcgcgaccg catcgtgctg gccggcaacg
      421 agagcgcggc tctggcgtcg ctggcgctgg gagccgatgg gctgattagc ggcctgagca
      481 cggccatccc ggagccgttc gtggccctga cccgcgccgc ggccgccggc caactagatg
      541 aagcccgcga atggcagcgc tgcatcaacc ggctgttggg cgttctgggc ggcgcgcgca
      601 ttggtggcat caaggccatt ctcgtccagc ggggcatccc tgtcggcccg cccgtgcctg
      661 tgctggacag caccgacgcg cccctctgga cgcgcgcggc cgagattctg gggatatagc
      721 gcccgctgac tatcgacggc cagccactga ccactgacca cttccgatgc gcctgattct
      781 cttcgacatt gacggcacgc tcatccacag cgaccgcatt ggccgggcgg cgatggcccg
      841 cgcgctaacg gacgtgttcg gccactgcgg cgcggtggac acaatctcgt ttggcggcaa
      901 gaccgactgc ggtatggtgc acgaactgat gctggccgag ggctggagcg agggggaggt
      961 cggggcgcgg ctgccccact tctacgagcg catggccgcc gccggacagg cactgtttac
     1021 ttcgacccac atccggccgt gccccggcgt cttgcccctg ctggcggccc tggccgcgcg
     1081 cgccgacgcc gtgcccgccc tgctgacggg caacatccag gcgacagcgc ccatcaagct
     1141 ggccgccgcg ggcatcgatc cggggctatt ccgcgtcggc gcctacggca gcgacagcgc
     1201 tatccgcgcc gacctctttg gcatcgctct ggcgcgagcc gaggccgagt tgggcttgcg
     1261 tatcgacgcg cacgatgtca tcatcgtggg cgacacgccg ggcgatattg attgcgcccg
     1321 cgccggcggc ggccgggtga ttgccgttgc cactggccca tacccggccg cgacgctgca
     1381 agagcaccgg cccgactatc tctttggcga tctgaccgcc acagacgccg tgctgagggc
     1441 tttattcgat gaccaccaag tctagccgcc agagagaacg cgcatcttcg cgcaagaaac
     1501 tcatgatcga cgatatcgct tttaacatcg gcgagccgac tcccataccc ctggagcgac
     1561 tttacacgtg ggtcgtctgg cagtttcccc gtctgcgctc cgtgacattg ggcggcaata
     1621 gcacgttcgg tgacgccgat ggagtcgaag gggacgtatt cacgggccac agcggcgctg
     1681 tacacccggg ggacggcgaa cacggctggt atccggcgct gatcgacgcc gcggccggca
     1741 ccgtcctcat cttcgccaac gtcgccgaac cctcccccag ccctgaggcg gcgtccaaat
     1801 acttcgacta accatgtgac gaacggcggg ccacccccat aacaaagagg cgcggcgtgt
     1861 gggcgttagc gccgccgccg agccggctag atcaccgtgc cgtcgggcac aaccgcgttc
     1921 ttgggcacaa tgacgatgcc atcgcgagcc acgtagcagt cgccctcttc gtcggggcgg
     1981 tcggggatat agcggatggt cacattgctg cccagccggg cgttcttgtc gatgatggcc
     2041 cctttgataa ccgcgttcgc gccaatgcca atgtcaggcc gcttgcgccg gcggttttcg
     2101 gcgcgatcgt cctcggtctc ataatagtca gcgcccatga caatcgacga ctggatgctg
     2161 gcctcctggc caataatcgc ccgcaagccg acaacgcact cgcgcacgct ggcatcgtag
     2221 agacggcagc cgtcggccag cagaacctcg tccagtcgcg tgttgtgcag ctcggagccg
     2281 ggcaggaagc gcgcgtgggt gtagatgggc gcttgggcgt cgtagaagtc aaacggggca
     2341 tcgattttgg ccatctgcaa attcacctcg aagaaccggc gaattgtccc gatgtcctcc
     2401 caatagccgt caaagacata gccgcagacg cgccgcttct tgatcgattg gggcaagatg
     2461 tggcgaccga agtcgacgta ttcctcttcc agcacagcct gcaagacgtc ggtgttgaag
     2521 acatagatac ccatcgacgc caggaagggt ttgtccgggt tggtcgggtt ctcaaagtcc
     2581 tggagcactt ccggcttctt gggcttctcg gcgaaggcag tgataacgtt gttactgtcc
     2641 atcttgagga tgcccaggcc actgactgtc tcgcgcgaga cgggttgcac ggcgacgctg
     2701 atatccgccc cgctctccac gtgctgaccc acgaattggc ggtaatccat gcgatagaga
     2761 tggtcgccgg ccaaaatcag gacgtacttc gcgcccgcct gcttgatctc catccactgc
     2821 ttgcgaacag cgtcggccgt gccctgatac cagtcggcgc tgcgcggcgt ctgttcggcg
     2881 gccagaattt gcacccagcc gggcgagaac tgatcgcgcg tgtaggtgct ccagatgtgg
     2941 cggtgcagcg aggccgagtt gaattgggtc aggatggcga tcttctcaat atcggcgtgg
     3001 aagcagttgc tcatgggaat gtcaatcagc cgatacttgc cggccagcgg gacagcgggc
     3061 ttggagcgct ccttcgtcaa cgggtaaagg cgtgttccct gccctccgcc taagattaag
     3121 cctagaacat cttccatata gggcatggcg tactccgaat gtgtgtttgt aggtgtgggt
     3181 catgccgcgg tcgcccgcgg catcaatact atagcgcggc ccttctgttt tggcacacaa
     3241 gcgccgctca tgctatgatt caggcgcaat tttggccggt cgtcgaaccg gctttctgcc
     3301 tgcccgacgg cacgcaagag gagtctacgt gagtcgagtc atccatgtcg ataaccctgg
     3361 caagcggcgc aacgcggccc gccgctccat tgccgaaatc ttgcgctacc tgagccgcaa
     3421 ggggcagatc gacgacgagg ccagagacat ggccgccgcc ctcgtctttc tgctgacaga
     3481 gatcaaggcg acggtcgatg aatcggccgc ggcctgggag aagcgcggct actggatgaa
     3541 ggttgagcgc ttcgtccgcg actacgagtg gatccccgga gcggcggcca acctcgacga
     3601 cgttatccgc catgacgcct gggatctgct gccggaactg ctggccgacc tgtcgcccca
     3661 cttcgacgac attcacatca agacgctgac gcgcaagcca accgagtggc gcggcgctta
     3721 tagccggcta aagaccacgc cgcccgtggc cgcgccctac tgagccaccg cggcgcgcgc
     3781 cgctctgccc ggctcttgtt gggggagtca tgaaggccat catcgttgcc cctatcctcc
     3841 tggcgctggc gctgttgctg ctgccggcct gcgggccaca gcccgcgctg ccgacgcgcg
     3901 cggcgccggc gctattcccg ccaacgccgc cggtgctccc gccgacgcca acgaccgcgg
     3961 gcgcgacaac cactgtcaac acgccggcgc ccacgtcgct gccagtcgct ccagtagcag
     4021 agccgcccct ggcaacagca gacgcggcca cgccgcttcc cctgccgtca ggcgaggccg
     4081 ctgctgccgt ccccaccggg gtatggccaa tcgcctcgcc caccccctgc gctacgcccg
     4141 gccgcgtcga ggcgggcgaa ctggccagcg ctgtggccgg ccagaccatg cgctatcggg
     4201 tctacctgcc accgtgctat ggcgaggatg ggcgcgtcta cccgaccctt tacctctttg
     4261 gcggcaacat ccacgacgag actttctggg acagactggg cttcgacgaa gcggctgagg
     4321 cggccatcgt cgccggccgc atcccgccgc tgctgatcgt catgcccgac aacggttggc
     4381 tggccaacac caccagcagc ggcccaacct cctacgaggg ttttgtcctc aacgagctga
     4441 ttccccacat cgaaagccag acctgcgcct ggccggcgcg cgaggggcgg gctgtcggcg
     4501 gcgtgtcgcg cggcggctat tggtcgctga tgatggcctt tcgccggccc gacctcttcc
     4561 gcagcgtggg ggcgcacagc ccggcgctga tcgacagctt cgccggcccg gacgaagatc
     4621 cccgcgtcac cggcacggcc aacccgctgg gcgacctgcg catctgggtc gatattggtg
     4681 agcgcgaccc gtacctgacc gcggctcgcc cgctccacga cacgctgacc gccgccggcg
     4741 tcgcccacga gtggcgcgtt aatcccggca tccacgacga agcctactgg acggcccatc
     4801 tggacgacta cataacctgg tacgccgccg gcttcccggc cgaccgcgcc gcgctgcccg
     4861 gctgctaggc ctgccagcgc gactgcgccg gccgcgcccc ggccaccgac cggtccgacg
     4921 accacggcgc atagggggcc atcgttagcg ccgccagagc gatgagcacg gtgtcatagt
     4981 tgactcgcca accgttgaag tcgcgccagg ccttttcgcg gtcggccagc aggggcacac
     5041 cggcggcggc cagctgcgcg caaacactgt cgtactcctc gcgagcaatg ctgatggggt
     5101 cgtcgggctt ggggttactg ttgtgctcga cgccgaagaa cgtggcgatt tcgcgcaggg
     5161 ccaggtagcc gctgcggatg cagaaagccg cccgtggttc ccaggggatg tccagcgttg
     5221 agagggtcag ggaggcggca tcgaggctga cgccggcggc cgttacccag gacaggtctg
     5281 gccgcggcga acgaaagaag gccagcgccg tcagtgacgt gtggctttcc tctaactccg
     5341 cgaaccacac ctgccagtcg ttccagaact cgcgcaactg ctctagctcg ccattgcgat
     5401 aggagcggat gagcatctcc caggccgacg gcggcgtgcc ggcgcgaact tccagcaact
     5461 gcacctgcgt ctcgcggcgg gtgaaggcgc tgtagatcgt cggcaggtag gcgatcagca
     5521 gggcgaccat gatcatgccc aagagggcct ggccgaactc gaagaccatc agcagcgggt
     5581 cgtcattggt gaaactaccc agcgtgaaca gcgccgaacc gctgagcttg atcgattcga
     5641 ggatgctcaa ggggctaagc gcccagtaga tcagggcaaa gccggtgaac accaggatca
     5701 tcaggacgat gggcacaaag aagagggcca tgggcgcgta gagggccatg atgcggtcgc
     5761 ggtcgttgta gctgatggcg cgccgcccac gccagttgaa tagccaacgg acggcggcga
     5821 atgtcagacg gatcgtccgc acctgcgccg ggcgtggcag gatgaaggtt ttgatggccg
     5881 ccaggacgct ggcggccacg atgtagaggc ccaacgccag cgccagggcc cgcgccgccc
     5941 acagcccgat cgcttctgcc tgcatagatg tctccctttg tgtcgccttt taggcgcgtg
     6001 cgctagtcta ggctagaatg ccgccagcgg ccaggaagcg ccgccgcggc taaccacctc
     6061 ccaccgacag accgagtgag cacgcccatg gactattccg attatcaaaa catcctcttt
     6121 gaacggcgcg agcatggtat cgtctggctg acgctaaacc ggccggaggc gctcaacgcc
     6181 gccgaccgcc gcctgcacac cgaactggtc gaggtctggc gcacgctcga ccgcgacccc
     6241 gacgcgcgcg tggccgtcgt caccggcgcg gggcgggcgt tcagcgccgg tggcgacctg
     6301 ggtatggtgg cggatgccta ccgcaacccg gaggagatcg gccgcatcct cgacgaggct
     6361 cgcgacctgg tctacaacat cctgcgctgc gacaagccca tcgtctcggc catcaacggc
     6421 ccggccgtgg gcgcggggct ggtcgtggct ctgctggccg acatcagcat cgtcgcggag
     6481 agcgcccggc tgggcgatgg ccacgtgcgc atgggcgtgg ccgccggcga ccacgccgcc
     6541 attgtctggc cgctgctggt gggcatggcc cgggccaagt actatctgat gacggctgag
     6601 ttcatcgacg gcaaggaggc cgagcgcatt ggcctggtca gcctgtgcgt gcccgacgag
     6661 gagctgctag agcgggcaca ggccgttgcc gtcaagttgg ccaccggccc gcgccacgcc
     6721 ctccgctata ccaagcgggc gctcaaccag tggctgctcc aggctgggcc gatcttcgac
     6781 cactcgctgg cgctggaaat gctgggcttc ttccatgagg atatggccga gggcgtggcc
     6841 gcgctgcaag gaaaacgggc ggcccgctat ccttcgagcg aaggctagaa accgggtttc
     6901 gcggcgaaac ccggctccta tggttgctag gtcacgcaac cgcgcgggcc acgcgaagca
     6961 ttgccgccag gctgcgcgtc acgtcctcgt ccgtcgttgc ccacgacgag acgctgatgc
     7021 gcatggccgt ccgcccctgc cagaccgtcg gcccgcacca gcacgtcccg tcggcctgga
     7081 tgccggccac cacccggctg gtcgtggccg cgtcgccaaa ggccacgacg acctggttga
     7141 gcaccacgtc gttgagcacc tcgtagccgg cagcctctaa ctcggcggcg aagcggcggg
     7201 catagccaca ggtgcgccgg atgaggtcgc gcacgccttc gacccccagc gctcgcaggg
     7261 ccgcccagac ctccacaccg cgcccgcgcc gcgacagctc cggggcaaag tcggacgggt
     7321 tgcgctcgcg gctggccgtg ggcaagtact cggcggtgat ggccatggcc gcccgcaggg
     7381 tgtcgctgtc gcgcacgaag gccacgccgc tgtcgtaggg cacgttgagc cacttgtggc
     7441 aatcgaccgc ccacgagtcc gccccggcca gaccgtcggt caggtgggcc atctccggca
     7501 cggccgcggc ccacaggcca aaggcgccat cgacgtgcag ccaggcccca ccggcgcggg
     7561 cgtgggcgca gatgtcggcc agggggtcaa acgccccgga gttgacgttg cccgcctggg
     7621 cgcagatgag cgtcggcccg ctgatgggcg gcagggcttc ggcgcgcagc cgcccctgcc
     7681 ggtcggcggg tacgcgcacg acgcggttgc ggcccagccc cagcaagctg agcgtcttca
     7741 gcagggtcac gtgggcttcc tggccgacga tgacggtgat cggcggcgcg ccgaagaggc
     7801 cgtcgttggc cacgttccag ccaacgcgct ccagcagccc gtagcgcgcc gcggccagag
     7861 cggtgaagtt ggccatcgtc gccccggtga caaagccgcc gcccgtcccg gccggcagcc
     7921 cgaagagcga caccagccac tccagggcga tgcgctccag ctgggccgtg gccggcgtga
     7981 cgttgtagag agccgagttc tggtcccacg ccccggccag ccagttggcc gccagcgcca
     8041 ccggcagcgc ccccccgatg acgaagccga agaagcgcgg cccggccatg gccatcgtcg
     8101 ccggcgagcc gatctcgtcc aagtcgcgca agacctcctc cggcggggtt ggttggtcgg
     8161 gcaggggcct gtccagttcg tccagccggt cgacgaccat cggcgcgacc gggcgggcgt
     8221 caaggccctc cagataggcg atcgcgcgct cagcagaatc agtcagtaac gaacgcatac
     8281 cttctccttg gctgtccccg ccaccgggta aggataagcg acttgcgcac ccatttcaac
     8341 ccccgcccac aggttgactt gctgagccaa tccctagtcg ttgtgtcgtt gggcgcgcct
     8401 ttgctctata ctagggacaa tccacaacga cggagtgtag gtcgatggct gacgaggcat
     8461 acaaggggca agtggcagcg ttcgaggccc gcatcgcccg cggcgagaag atcgaaccgg
     8521 gcgattggat gcccgacgcc taccgcaagc agctcatccg catgatttca cagcacgccc
     8581 atagcgaggt cgttggcatg ctgccggaag gggtgtggat cacccgcgcg cccaacctgc
     8641 ggcgcaagat ggtgctgctg gccaaggtgc aggacgaggc cggccacggc caatatctct
     8701 atcacgccgc cgagacgctg ggcgtcggtc gcgacgagat gatcgctgcc ctgctcgacg
     8761 gccgggccaa gtacgccagc gtctttaact accccaccct cggctgggcc gacgtggccg
     8821 ccattggctg gctggtcgat ggcgcggcga tcatgaacca gaccatgctg gcccactgct
     8881 cctacggccc ctactcgcga gcaatggtgc gcatctgcgc ggaggagggc ttccacaaga
     8941 agcaaggcca ggagatgatc atccgctacg ccgccggaac ggcgacgcaa cgggccatgg
     9001 cccaggaggc catcgaccgc tggtggtggc cggcgctgat gatgttcggc ccgcacgaca
     9061 gcgcgtcgag caacacgccg cagctcgtcc gctggggtat caagaccaag accaatgacc
     9121 agctgcggca agagttcatc gacgacatgg ttcccgcgct gcaaaccctc ggcctgagcg
     9181 tacccgaccc ggagatgcgc tacgacgccg ccaccggcca ctgggccggc cgcgagatcg
     9241 actgggacga gttctggcgg gtcatccagg gcgatggccc catgaacgcc agtcgcctgg
     9301 acgcccgccg gcaagcgcac gaggacggcc gctgggtgcg cgaggcggcg gcggcgtatg
     9361 gagagaagca gccggggagc gggagtgcgg gcgagcaggg gagaaaagag tcactcctgc
     9421 ccccctgcgc ccccgccccc ctgcatcctt ccgagagcac tacatgaacg accagtggcc
     9481 ccgctatgaa gtcttcaagc aggagcgcga cggcgggccg tttcgcaacg tcggctccgt
     9541 ccacgcgccg gacgcggaga tggccctgct gaatgcccgt gacgtgttcg cccggcggcc
     9601 ggcctgccgg tgtctgtggg tcgtacccgc cgcggccatt gtcgccacgc ccatagtcgt
     9661 caacgccgcc ggcgacgcgg agccggacga cgtctccggc ccgttagagc cgtatgccgt
     9721 ctgcggcaag tcgtcggagc ggcaggcgat gacctacgtg gactatctcg gtcgcgtcga
     9781 ggcgcgctcg gccgccgaag ccctgcgggc cgcgccggcg ctgggcgcgg cgcgcatctg
     9841 gtgggtcttc ccggagcggg tcatcagccg cagcgatccg gaggacgcgc ccggcctgga
     9901 agcagccacg ggcaagctct tccgcatgtc caaccagtac cataccgtcg tcaccatgca
     9961 aaagattcgc cgcggcgacg agggcgaaga atgatcgacc ccaccctggc cgtgccgctg
    10021 gccgaccggc tgttggcaat ggccgacgac gagttgatcc tggcccaccg caactcggag
    10081 tgggccggcc acggccccat cctggaggag gacatcgcct tcgccaacat cgccctggac
    10141 gagatgggcc acgccgccat ctggtatggc ctgcgcagcg acatcaccgg cgaggacacc
    10201 gaccggctgg tcttctttcg caatgccggc cagtatcgca acgtccagat ggtcgaactg
    10261 ccgc
//
LOCUS       DAOSMQ010000049        10259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6529_length_10259_cov_4.069777, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000049 DAOSMQ010000000
VERSION     DAOSMQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6529_length_10259_cov_4.069777"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..708)
                     /locus_tag="PK829_02880"
     CDS             complement(82..708)
                     /locus_tag="PK829_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40191.1"
                     /translation="MNDMTTGDGPIGSRPAGETAPGQPSESPLADAPIEASPDLSEQL
                     ADPASSPASDDAAVETSPAAETDEWPGALPASGPDESSPDGAPPNVTPVAGQPPLPEA
                     RRLSRGEREVVERARQRFAACGRCGYLIADLQILLDETTLQTGMIQARDGWLRLESDW
                     RLRALLSNAYGVRLGLDYDMMDGTCPECRRRFVFAALDDGRARLKLHL"
     gene            834..2018
                     /locus_tag="PK829_02885"
     CDS             834..2018
                     /locus_tag="PK829_02885"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HOU40192.1"
                     /translation="MEIRSVTLFCDAGRSPAVYAPFLKAARGAFPVRVQSMRLGTTPF
                     PDWLPRRDAARAAALAEAWRAAGVDYVSLGPVQLRHDPAWLAVIPDLLADTDGVFASI
                     EIATTDGHIDTGRCRDAAEIIRRVSTLRPDGFANLFLGALAACGPGHPFLPASYAGGA
                     DTFAIAVEAADVALTAVNGAATLTEAAGRLTAGIEAAAAAIVPVAQRLSAEFNLPFGG
                     LDFSLAPYPTPDKSLGAALEALGTRVGAPGALFAAAFITAAIDRAAFPRTGFSGLMLP
                     VLEDATLARRAAAGQLALNDLLLYSAVCGVGLDTIPLPGDIGAATLAGILLDTAALAA
                     RLRKPLVARLMPLPGHRAGDETAFSFEYFAPGRAMTVSGEGVGGLLGGRTALALAANR
                     TR"
     gene            2026..3195
                     /locus_tag="PK829_02890"
     CDS             2026..3195
                     /locus_tag="PK829_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU40193.1"
                     /translation="MRIAMIGPFGLHPKQTMRSRALGLARPLVARGHAVRLFMPPWDT
                     PDEAGRTWTEDGVELRYVPLRGGVPGIAWVLARETLAWRPDVVHAFKPKAYSGLAAAW
                     LWRFHRDRLRLVMDTDDWEGAGGWNDLAPYSPLQKRFFARQERYGLTHAHAVTVASRT
                     LQSLVWSLGMSPERVVYVPNGPGISMHAVSSAERAAERARLGLGRRPAVLVYSRLFEF
                     DTARLAAALAGIRAVVPDVAVLLVGAGLYAADSAAFRARLADAGLLDAVIDVGWTARE
                     QLPATLAAADVGLYLMDDTLLNRTKCPVKLADMLVAGVPVVAEAVGQVSEYIRDGRTG
                     ALAASGDVAGLVGATVALLADAGERTRRGAAAREDIVTRFGWAGLAGTLEGVYAG"
     gene            complement(3310..3750)
                     /locus_tag="PK829_02895"
     CDS             complement(3310..3750)
                     /locus_tag="PK829_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOU40194.1"
                     /translation="MSVAEPAELFFLDTNVLIYVFDRTAPEKQRVAEQLVRHAVTTGQ
                     GLISTQVVQEFLHAARRKFERPMTLDERRQHLEMVLAPLCQYSPSISFYDHALLIAEE
                     TGYAFYDALIITAAVESGCRVLYSEDLQHDRVVRGVRLVDPFRD"
     gene            complement(3737..3979)
                     /locus_tag="PK829_02900"
     CDS             complement(3737..3979)
                     /locus_tag="PK829_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40195.1"
                     /translation="MLKNITFSADENLIRMARRQAALEDTTLNQLFREWLERYVVQQD
                     SEMRRQAVARYDALMGRLAHVNAGRKFTREELNERR"
     gene            4194..>5369
                     /locus_tag="PK829_02905"
     CDS             4194..>5369
                     /locus_tag="PK829_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40196.1"
                     /translation="MSRSLSLLAFVLLLAACNRAQTDNTGPATVTPEPASPTTVPTQS
                     PPPTAAPTETSAPALPVDATPLDGQWEGAIDIAGQQLGIIVKFDSSTGALTATIDIPQ
                     QGAADLPLNDVRLEGDAVHFAIGSVGAVFDGTLDGETIRGAFAQAGATGTFEIARTGE
                     VVAATPTPIPPYVVTEVSWPLDDTTVGATLTRPEGDGPFPAVVFVAGSGPTDRDWTSP
                     LLPGRNGSAALLADVLTRAGYATLRYDKRFTGPFAQQNMPKLLGNISFQSHLDELASA
                     VDYLVAQPGIDPAQVFVLANSEGTLHAMNYQTSDPATPFAGLILTGAPGRPLTEVLHQ
                     QISENVLSAEPNRDELLVLWDEAIAAFLAGETATLDPALPESAQQVFAGLVAPANQPF
                     "
     assembly_gap    5370..5379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5380..5713
                     /locus_tag="PK829_02910"
     CDS             <5380..5713
                     /locus_tag="PK829_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40197.1"
                     /translation="GAASGALLNAELLAVQPAELLADITAPTLVIIGQKDIQIDWQAD
                     GPVLAAAAGDNVTFSYPANANHVLKLEEKPAGELTAADGANYNAADRVLDPETVQIIL
                     DWLAERTN"
     gene            complement(5856..6020)
                     /locus_tag="PK829_02915"
     CDS             complement(5856..6020)
                     /locus_tag="PK829_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40198.1"
                     /translation="MLRGGSWNNNQRNARASIRNNNTPDNANNNIGFRPVAHVFTSEA
                     QPAMLRVIAR"
     gene            complement(6068..6412)
                     /gene="avd"
                     /locus_tag="PK829_02920"
     CDS             complement(6068..6412)
                     /gene="avd"
                     /locus_tag="PK829_02920"
                     /inference="COORDINATES: protein motif:HMM:NF033474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diversity-generating retroelement protein Avd"
                     /protein_id="HOU40199.1"
                     /translation="MEQSPIFTKTYDLLTWLLPATTKFPREHRFVMAKAVQETALRFQ
                     ERLIEAGLGSDRTRQHALAQADVELTKLRFYLRLSRDMSLITPRQYQHVAEYTTEVGR
                     LLGGWRHSLKAA"
     gene            complement(6448..7500)
                     /locus_tag="PK829_02925"
     CDS             complement(6448..7500)
                     /locus_tag="PK829_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HOU40200.1"
                     /translation="MKTYKHLYPAVCEWDNLDLAYRKARHGKRSRPPAAAFEYDLEAN
                     LVVLRRELADRTYQPGRYHSFYIHEPKRRLISAAPFRDRVVHHALCNVIEPLFERSFI
                     ADSYANRIGKGTHRALDRAQQYARRFPYVLQCDVRQFFPSIDHALLRVILARKIADPD
                     VMWLVDGILDSGRGVLSEEYEMQWFPGDDLLAALRPRGLPIGNLTSQFWANCYLNPFD
                     HFIKRELGCPGFVRYVDDLLLFGPDKATLWDWRQAVVEKLAGLRLTIHSGAQPRPVTE
                     GIPFLGFVVFPDYRRLKRRQGIRYRRRLGALLRQYAAGEIGIDRVHASVRGWINHVRY
                     GDTWRLREAMLGPVVV"
     gene            complement(7595..>10259)
                     /locus_tag="PK829_02930"
     CDS             complement(7595..>10259)
                     /locus_tag="PK829_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU40201.1"
                     /translation="LYSLRRALATHENWGPLITYTDSEVVRLQLRQATTSLAHIEANL
                     RALLAVFGVTDRDEESALRDYLEHVLRETAAISFLFVKPAGPRHKLKTEAELEAVYVP
                     LQVQDPDRPAEQARAQNRRAPKLAADDLEPAEARTPTLTIDQVLARHAIFLLRGKPGS
                     GKTTLLRHLAHTFAAGQAAERLAWTGEPLLPILVPLRNFGRFLDDHRKEYASPAPLAL
                     RNFIEDYFKGHELGLPPDFFHRRLDQGHCLVLLDGLDEVADKPLRATVAQYVSSFLKH
                     YEPKGNRFGLSSREKGYDEVAAYLPRPIVCNVQDLEPDGRDHLVRNLLQQFDDDPRRC
                     REETADLLHDIANRRQVDSLSRNPLFCTTLVLVYKYRGRKLPERRVDVYQEVVSLMLG
                     FWETQRTGVAEARELALMDGTGRSFMDEFEAIEVKQRTLTDLADWMQQEAREAGVQKE
                     EVLAHLATFFSEREGAQADQKSDWARGFLDVAHQRSGLFIEIDPGVYAFSHGHFREYL
                     AATALVDRTDDEMVRAVLAHAAEGWWEEVIKLAVAHERLSPSRRELLLRAMIDGGHLV
                     LAGWCAVDAGSGRLPAPLRERIKSELRARMVDGKRLPPDRFAAGEALDALGWLPEDLN
                     GWVRCPASADDRRDLLAGRYPVTNAQYELFLRAGGYDDPAYWGGEQSEGWQWRTGGER
                     PVSSGKNRDEPEYWQSANFGRDRRGFPVVGVCWYEAAAYCGWLTALLRRARAGEELPP
                     AHLALIAGLVDAGAAEARFPSEREWVRLAGGEADDRYPWDGPAGATTDKATILARANT
                     SESAINNTSPVAMYPQGSSRPFGLQDMAGNVWEWTASWVDEKQSGRVLRGGSWHYHQR
                     YARASIRHDNTPVVAFNNSGFRPVAPVDSGN"
BASE COUNT         1603 a         3499 c         3473 g         1674 t
ORIGIN      
        1 ttccgcgcaa acgggggcga cccgtctgct attggggtgc ggtggggtgc gcggggaaga
       61 tagacggggc ggcgacccgt cctacaggtg cagcttgagg cgcgcccggc cgtcgtccag
      121 ggcggcgaag acgaaccgcc gccggcactc cggacaggtc ccgtccatca tgtcatagtc
      181 caggcccaga cgcacgccgt aggcattgct caacagcgct ctcagccgcc agtcgctctc
      241 cagccgcagc caaccgtcgc gggcctggat catgcccgtt tgcagggtcg tctcgtctag
      301 caggatctgc aggtcggcga tcaggtagcc gcaacggccg caggcggcga agcgctggcg
      361 cgcccgctcg accacctcac gctcgccgcg ggagaggcgg cgcgcttccg gaagcggagg
      421 ctggccggcg acgggcgtaa catttggcgg cgcgccgtcc ggcgacgatt cgtccgggcc
      481 ggaggcaggt agcgcgccgg gccactcgtc cgtctccgcc gcgggagagg tctcgacggc
      541 cgcgtcgtcg gacgctggcg aactggccgg atcggccagc tgctcgctca agtctggcga
      601 ggcctcgatg ggggcgtcgg ccagggggct ttcgctgggc tggcccgggg ccgtttcgcc
      661 ggccggccgg ctgccgatgg ggccgtcgcc cgtggtcatg tcgttcatgt gcgcgccgtt
      721 gcctgtcaga aacgccccgt ctggacagac agtggggcga atgtgtgaat tccgttcaat
      781 ctgtggttta ttacgcgata aggccattct aacggcaagg caacggctgt caaatggaaa
      841 ttcgatctgt caccctgttt tgcgatgccg gccgctcccc ggccgtctac gctcccttcc
      901 tgaaggccgc ccgcggcgcg ttccccgtgc gcgtccaatc gatgcggctg ggcaccacac
      961 cctttcccga ctggctgccg cgccgggatg ctgcccgggc cgctgctctg gccgaggcct
     1021 ggcgcgcggc cggagttgac tacgtcagcc tcggcccggt gcagttgcgc cacgacccgg
     1081 cctggctggc ggtcatcccc gatctgctgg ccgacaccga cggcgtcttt gcctccatcg
     1141 agatcgccac gaccgacggc catatcgaca ccggtcgctg ccgcgatgcg gcggagatta
     1201 tccgccgcgt cagcacgctg cggcccgatg gcttcgccaa cctcttcctg ggcgcgctgg
     1261 ccgcctgcgg ccccggccac cccttcctgc cggccagcta cgccggcggc gccgacacct
     1321 tcgccatcgc tgtcgaggcg gccgacgtcg ccctgacggc cgtcaacggg gcggcgacgc
     1381 tgactgaggc ggccggccgg ctgaccgcgg gcatcgaggc cgccgccgcg gccatcgtgc
     1441 ccgtcgccca acggctgtca gccgagttca acctgccctt cggcggcctc gacttctcac
     1501 tggctcctta cccgacgccc gacaagagcc tgggcgcggc gctggaggcg ctggggacgc
     1561 gcgtcggcgc gccgggggcg ctcttcgcgg cggcgttcat caccgcggcc atcgaccggg
     1621 ccgccttccc gcgcaccggt ttctccggcc tgatgctgcc cgtgctggag gacgctacgc
     1681 tggcccggcg ggcggccgcg ggccagctgg ccctcaatga cctgctgctc tactcggccg
     1741 tctgcggcgt cgggctagat accatcccac tgcccggtga catcggcgcg gcaacgctgg
     1801 ccggcatcct gctggacacg gcggcgctgg ccgcccggct gcgcaagccg ctggtggccc
     1861 ggctcatgcc cctgcccggc caccgtgccg gcgacgagac cgcgttttcg ttcgagtact
     1921 tcgcccccgg ccgggccatg actgtcagcg gggagggcgt cggcgggctg ctgggcggca
     1981 ggacggcgct ggccctagcc gccaaccgga cgcgctaggc ggcctatgcg catcgccatg
     2041 atcggcccgt ttgggctaca ccccaagcag acgatgcgca gccgggcgct ggggctggcc
     2101 cggccgctgg tggcccgcgg ccacgccgtg cgcctgttca tgccgccgtg ggacacgccc
     2161 gacgaggccg gccggacgtg gacggaggat ggcgtcgagt tgcgctacgt gcccctgcgc
     2221 ggtggcgtgc ccggcattgc ctgggtgctt gcgcgcgaga cgctggcctg gcggccggac
     2281 gtcgtccatg ccttcaagcc caaagcctac agtgggctgg ccgcggcgtg gctgtggcgc
     2341 ttccaccgcg accggctgcg gctggttatg gataccgacg actgggaagg ggcgggcggc
     2401 tggaacgacc tggcacccta ctcgccgctg caaaagcgct tcttcgcccg gcaggagcgc
     2461 tatggcctga cccacgccca cgctgtcacc gtcgccagcc ggacgctgca gagcctggta
     2521 tggagcctgg gcatgtcgcc agaacgggtc gtctatgtgc ccaacgggcc gggtatctcc
     2581 atgcacgccg tctcgtccgc ggagcgggcg gccgaacggg cgcggctggg gttggggcgg
     2641 cggccggcgg tgctggtcta tagccgcctg ttcgagttcg acacggcccg tctggcggca
     2701 gcgctggccg gcatccgcgc tgtcgtgcct gatgtggccg tgctgctggt cggcgcgggc
     2761 ctctacgccg ccgatagcgc cgccttccgc gcccggctgg ccgacgccgg actgctggac
     2821 gcggtcatcg acgtgggttg gacggcgcgg gagcagttgc cggcgacgct ggccgcggcc
     2881 gacgtgggcc tctacctgat ggacgacacg ctgctcaacc gcaccaagtg cccggtgaag
     2941 ctggccgaca tgctggtcgc gggcgtgccc gtcgtggccg aggcggtcgg gcaggtgagc
     3001 gagtatatcc gcgatgggcg gacgggggcg ctggccgcgt cgggagacgt ggcgggactg
     3061 gttggcgcga cggtggcgct gctggcggac gccggcgaac ggacgcgccg gggcgcggcg
     3121 gcgcgggagg acatcgtgac gcggtttggg tgggcggggc tggccgggac gctggaaggt
     3181 gtttatgccg gttagccggc ggccgcggcg gttgtgcttc gcagtctaga ccaccgtcgc
     3241 caacggctca atggccctgg ccagtcgatt aagcgccagg tggattctgg ggcaggcgac
     3301 cgagggccgc tagtcgcgaa acggatcgac caggcgcacg ccacggacga cgcggtcgtg
     3361 ctgaaggtct tcgctataga ggacgcggca gccgctctcg acggccgcgg tgatgatcag
     3421 ggcgtcgtag aaggcatagc ccgtctcttc agcgatgagc aaggcgtgat cgtagaacga
     3481 gatcgatggg gagtactggc acagcggcgc taagaccatc tccaggtgtt ggcgtcgctc
     3541 atccagggtc attggccgct cgaacttacg tcgtgcggca tggagaaatt cctgcacgac
     3601 ctgggtactg atcaggcctt gacccgtagt cactgcatgg cgcacgagtt gctcggccac
     3661 acgctgcttt tcaggtgcag tccggtcaaa cacatagatc aggacgttcg tatcaagaaa
     3721 gaacagttcg gccggctcag cgacgctcat tcaactcctc gcgggtgaac ttgcgcccgg
     3781 cattgacatg agccaggcgt cccatcaagg cgtcgtagcg ggcgacagcc tgccggcgca
     3841 tctcgctatc ctgctggacg acgtaccgct ccagccattc gcggaacaac tggttgagcg
     3901 ttgtgtcctc cagcgccgcc tgccgtcgcg ccatgcggat taggttttca tcggccgaga
     3961 aggtgatgtt cttgagcata ggcccagtgt acacaagaat cgtgtacaca gcaagacgac
     4021 acagggcttt agggcttttc caaagtcacc cccggcgcac cgacctgaca ggggagcggc
     4081 cacgaatcgt acttgcacca gaagacgact ggccgctcac ctgtcaggtc tgcgttcaat
     4141 ctcatggtat aatgagcagt tgactgccta cccttaaccg aggtcagcta accatgtcac
     4201 gttcattgtc ccttctggct ttcgttttgc ttctggccgc ttgcaatcgg gcccagacag
     4261 ataacacggg gccggccacc gtcacgcccg aacctgcgtc gccaacgacc gtgcccaccc
     4321 aatcgccccc gccgacggcc gcgccaaccg agaccagcgc gccggccctg cccgtcgacg
     4381 ccacgccgct ggatggccag tgggaagggg ctatcgacat cgccgggcag caattgggca
     4441 tcatcgtcaa gttcgactca tccaccggcg cgctgacggc gacaatcgac atcccccagc
     4501 agggcgcggc cgacctgccc ctcaacgacg tccgcctgga gggcgacgcc gttcacttcg
     4561 ccatcggctc tgtcggcgcg gtgttcgacg gcacgctcga cggggagacg atccgcggcg
     4621 ccttcgccca ggcgggcgcc acgggcacct tcgagatcgc ccgcaccggc gaggtggtcg
     4681 cggccacgcc cacgcccatc ccgccctacg tcgtcaccga ggtcagctgg ccgctggacg
     4741 acacgaccgt gggcgcgacg ctgacccggc cggagggcga cggccccttc ccggcggtgg
     4801 tcttcgtcgc cggcagcggg ccgaccgacc gcgactggac ttcgccgctt ctgcccggcc
     4861 gcaacggcag cgccgccctg ctggccgacg tgttgacccg cgccggctat gccacgttgc
     4921 gctatgacaa gcgcttcacc ggcccctttg cccaacagaa catgcccaag ctgctgggca
     4981 acatcagttt ccagagccat ctggatgagc tggccagcgc cgtggactat ctcgtcgccc
     5041 agccgggcat cgacccggcg caggtctttg tgctggccaa cagcgaggga acgctgcacg
     5101 ccatgaacta ccagacgtcg gacccggcca cgcccttcgc cgggctgatc ctgacgggcg
     5161 cgcccggccg gccgctgacc gaggtgctgc accagcagat cagcgagaac gtgttgagcg
     5221 ccgagccgaa ccgtgacgaa ctgctggtgc tgtgggacga ggccatcgcc gccttcctgg
     5281 ccggcgagac agccacactc gacccggcgc tgccggagag cgcgcagcag gtcttcgccg
     5341 ggctggtggc gccggccaac cagccgttcn nnnnnnnnnc ggcgcggcca gcggcgcgct
     5401 gctcaacgcc gaactgctgg ccgtgcaacc ggccgaactg ctggccgaca tcaccgcgcc
     5461 aacgctggtc atcatcggcc agaaggacat ccagattgac tggcaggccg acggcccggt
     5521 gctggccgcg gccgccggcg acaacgtcac cttcagctac ccggccaacg ccaaccacgt
     5581 gttgaagctg gaggagaaac cggccggcga actgacggcg gctgatggag cgaactataa
     5641 cgccgccgat cgggtgctgg acccggagac ggtgcagatt atccttgatt ggctggcgga
     5701 gcggaccaac tgattgaaga tgcgcccggc cgcgtgcgtc gcatgacgct accacaggcg
     5761 gccgggcgtt tttctatttg cccgcgcccg ggtcccaagc ggggccgggc acggccagga
     5821 acaggttacg ccgtcttttt aagcctcggc tcgcgtcacc tggcgatgac ccggagcatt
     5881 gccggctggg cttcactggt aaagacgtgg gcgaccgggc ggaaaccgat attgttgttg
     5941 gcgttgtccg gcgtgttgtt gttgcggatc gacgcacgcg cgttcctctg attgttgttc
     6001 cacgacccgc cgcgcagcac gcggccacct gataacctgc ccccgcgtat ggacactcgc
     6061 gcgcccctta cgccgctttc aaggagtgtc gccagccgcc cagcagccgg ccaacctccg
     6121 tcgtatactc cgccacgtgt tgatactgtc gcggcgtgat gagactcata tcgcgcgaca
     6181 gccgcaagta gaaccgcagt ttcgtcagtt cgacgtcggc ctgggccaag gcgtgttgcc
     6241 gcgtccggtc gctgcccaat ccggcctcga tcagccgctc ctggaaacgg agtgccgtct
     6301 cctgcaccgc cttcgccatg acgaagcgat gctcacgtgg gaacttcgtc gtcgccggca
     6361 gcagccacgt caatagatca taggtcttgg tgaagatagg cgattgctcc atatccgtct
     6421 ccccgaatga ccgaatggcg ccaaggccta cacgaccaca gggccgagca tggcctcgcg
     6481 caaccgccag gtgtcgccgt agcgaacatg attgatccag ccgcgcacag aagcatgcac
     6541 acggtctatg ccgatctcgc cggccgcgta ctgccgcagc agtgcgccga gccgccggcg
     6601 gtagcgaatc ccctgccgcc gtttgagccg ccggtagtcg ggaaagacga caaagcctag
     6661 aaacgggatg ccttcggtca ccgggcgggg ctgagcgccg gaatggatcg tcagacgcag
     6721 cccggccaac ttctcgacaa cagcctgccg ccaatcccac agggtcgcct tgtccgggcc
     6781 gaagagcagc agatcatcga cgtagcgcac gaagccgggg cagcccaatt cgcgcttgat
     6841 aaagtggtcg aacgggttca ggtagcagtt ggcccagaac tggctggtca ggttgccgat
     6901 gggcagcccg cgcggacgga gcgcggcgag caggtcgtcg ccggggaacc attgcatctc
     6961 gtactcctcc gacagcacac cccggccgct gtcgaggatc ccatccacca gccacatcac
     7021 gtccggatcg gcgatcttgc gcgccaggat aacccgcagc agcgcgtgat cgatactggg
     7081 gaagaactgg cgtacgtcgc attgcaggac gtagggaaag cggcgcgcat actgctgcgc
     7141 ccggtcgagc gcccggtggg tgcccttgcc gatgcggttg gcgtaggaat cagcgatgaa
     7201 gctgcgctcg aagaggggct cgatgacgtt gcacagcgcg tggtggacga cccggtcgcg
     7261 gaaaggggcg gcggagatca gccgccgctt gggttcgtgg atgtagaacg aatgatagcg
     7321 gcccggttgg tacgtcctgt ccgccagctc gcggcgcaac acaaccaggt tggcctccag
     7381 atcgtattca aacgcggctg ccggcgggcg gctgcgcttg ccgtgacgcg ctttgcggta
     7441 ggccagatcg aggttatccc actcgcagac ggcgggatag aggtgtttgt aggtcttcac
     7501 aggggcatct cccgttcgcc ccgacaacca cttttgcgcg gcgctggggg gcaagccccc
     7561 cagacccccg cttcaggaat cagtatacca gaattcagtt gccagaatca acgggggcga
     7621 ccgggcggaa accgctattg ttgaaggcga cgaccggcgt gttgtcgtgg cggatcgacg
     7681 cacgcgcgta cctctgatgg tagtgccacg acccgccgcg cagcacgcgg ccactctgct
     7741 tctcgtcgac ccacgacgcc gtccactccc agacgttgcc ggccatgtcc tgcaagccga
     7801 acggccggct gctgccctgg ggatacatcg ctaccggcga ggtgttgttg atagctgact
     7861 cgctggtgtt agcgcgggcc aggatggtcg ccttatctgt cgtcgccccg gccggcccgt
     7921 cccacgggta gcggtcatct gcttcgccgc cggccaaacg cacccactcc cgctccgacg
     7981 ggaagcgggc ctccgctgca ccagcgtcga ctagcccggc gatgagcgcc aggtgcgctg
     8041 gcggcagttc ctctccggca cgggcgcgcc gcaatagcgc cgtcagccag ccgcaatagg
     8101 cggcggcctc gtaccagcag acgccgacca cggggaagcc gcgccgatcg cggccaaagt
     8161 tggctgactg ccagtactcc ggctcatccc tattcttccc gctactcacc gggcgttcgc
     8221 ctccggttcg ccattgccag ccctcgctct gctcgccgcc ccagtacgcc ggatcatcgt
     8281 agccgccagc gcgcaggaac agctcgtact gggcgttggt caccgggtag cgcccggcca
     8341 acaggtcgcg tctgtcatcg gccgacgccg ggcagcgtac ccagccgttc aaatcctccg
     8401 gcagccagcc cagcgcgtcg agggcttcgc cggcggcgaa acggtctggg ggcaaacgct
     8461 tgccgtcaac catgcgcgcc cgcaactccg acttgatccg ctcgcgcagc ggggcgggca
     8521 ggcggccgct accagcgtcg acggcgcacc agccggccaa caccaggtgc ccgccatcaa
     8581 tcatggcccg cagcagcagc tcgcgccgcg agggcgacag gcgctcgtgg gccacggcca
     8641 gcttgatcac ctcctcccac cagccctcgg cggcgtgggc cagcacggcg cgcaccatct
     8701 catcgtcggt gcggtcgacc agggctgtgg ccgccagata ctcgcggaag tggccgtggg
     8761 agaaggcgta aacgccggga tcgatctcga tgaacaggcc gctgcgctgg tgggccacat
     8821 ccaggaaacc gcgcgcccag tcgctcttct ggtcggcctg cgcgccctcg cgctcgctga
     8881 agaaggtcgc cagatgcgcc agcacctcct ctttctgaac gcccgcttcc cgcgcctcct
     8941 gttgcatcca gtcggccagg tcggtcaggg tgcgctgttt gacctcaatg gcctcgaact
     9001 cgtccatgaa tgaccgcccc gtgccgtcca tcagggccag ttcgcgcgcc tcggccacgc
     9061 ccgtccgctg cgtctcccag aagcccagca tcaggctgac gacctcctga tagacgtcta
     9121 cccgccgttc gggcagcttc cggccgcggt acttgtagac cagcacgagc gtggtgcaga
     9181 agagcgggtt gcgcgatagg ctgtcgacct gccgccggtt ggcgatgtcg tgcagcaggt
     9241 cggctgtctc ctcgcggcag cggcgggggt cgtcgtcgaa ctgctgcaac aggttgcgga
     9301 caaggtggtc gcggccgtcc ggctccaggt cctgtacgtt gcagacgatg ggccggggta
     9361 gataggccgc cacctcgtcg tagcccttct cgcgcgagga cagaccgaag cggttgccct
     9421 tcggctcgta atgcttcagg aagctgctga cgtactgagc cacagtcgcc cgcaggggct
     9481 tatcggcgac ctcatccagt ccatcgagca gcacgaggca atgaccctga tccagacggc
     9541 ggtggaagaa gtcgggcggc aagcctaatt catgcccttt aaagtagtct tcgatgaagt
     9601 tgcgcagggc caacggcgca gggctggcgt actccttgcg gtggtcgtcc agaaagcgtc
     9661 cgaagttgcg cagcggcacg aggatgggca gcaggggttc gcccgtccag gccagccgtt
     9721 cggccgcctg cccggcggca aaggtgtggg ccaggtggcg cagcagggtg gtcttgcccg
     9781 atccgggctt gccgcgtagc aggaagatgg cgtggcgggc cagaacctgg tcgatagtca
     9841 gcgtcggcgt gcgggcctca gctggctcta aatcatccgc tgccagtttg ggagcgcgac
     9901 ggttctgagc gcgggcttgc tcggccgggc gatcgggatc ctgaacctgc agcggcacgt
     9961 agaccgcctc cagttcagcc tcggtcttca gcttgtggcg cggcccggcg ggcttaacga
    10021 aaaggaatga gatggccgcc gtctcgcgca ggacgtgttc caggtagtcc cgcagcgcgc
    10081 tttcctcgtc gcggtcggtg acgccgaaca cggccagcag ggcgcgcaga ttggcctcga
    10141 tgtgggccag gctggtcgtc gcctgacgca attgcaggcg gacgacttcg ctatcggtgt
    10201 aggtgatcag cggcccccag ttctcgtgcg tcgccagcgc ccggcgcagg ctgtagagg
//
LOCUS       DAOSMQ010000050        10246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6535_length_10246_cov_5.067118, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000050 DAOSMQ010000000
VERSION     DAOSMQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6535_length_10246_cov_5.067118"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4522)
                     /gene="gltB"
                     /locus_tag="PK829_02935"
     CDS             complement(<1..4522)
                     /gene="gltB"
                     /locus_tag="PK829_02935"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOU40202.1"
                     /translation="MGRSQRRKSGQQPPERDVASGEGPAQQGLYDPRYEHDACGTGFV
                     AHVKGQRSHAIVADALTVLDNLSHRGARGSEPNTGDGAGILTQLPHAFFAAEAARLGF
                     HLPPAGEYGVGMLFLPRDPARRLACERQLAGLVAAEGHTLLGWRDVPTDNRALGATAV
                     ASEPVVRQVFIGRGPATGAAGDALAFERALFVLRRLAERAIDSPAGGAQVFYVVSLSS
                     RTLVYKGMLLAEQLRDYFPDLSDPRFDSAIALVHSRFSTNTFPAWKRAHPNRILAHNG
                     EINTIRGNVNAIKARQALFRSDAFGPDLARVLPVIDEDGTDSAMFDNALEFLVLAGRS
                     LPEALMMMIPEPWEKHAGMSAAKRAFYAYHSALMEPWDGPAAIVFSDGALVGAVLDRN
                     GLRPARYTVTADDRVIMASEVGVLDIPPEQVLHKGRLQPGRMLLVDTAAGRIVADEEL
                     KETIAAARPYGDWLDEHLLPLEALPVAGDEPVPDHDTVTIRQHAFGYTFEDLRLIMAP
                     MARDGVEPLGSMGDDTPVAVLSERPQLLYNYFRQLFAQVTNPPIDAIREELVTSSTML
                     LGAEGDLLNPRPEDCCRIRIPSPLLTNAALASLRAIRVPGFRAATLPILYPAGGDGAA
                     LTAALAELCAAAGKAVAGGANLLILSDRGVGRARAPIPALLAVSALHEHLIAAGTRTR
                     VSLLVESGEPREVHHYACLVGYGADAVNPYLAFETLHDLIARRLLDVEYDTAAAKYLK
                     AAVKGVVKTISKMGISTVQSYHGAQIFEALGLSRAVVDRYFSGTPTRIEGADMDVLAA
                     EAAQRHAAAYPARPANGHTLEVGGRYQYRSGGEFHLFNPSTIHKLQHAVRSGDYRAYK
                     QYADEVHARETHLATLRALLEFQFAPTPVPLDEVEPVEAILSRFKTGAMSYGSISREA
                     HETLAIAMNRVGGKSNTGEGGEDPARYAPGPDGDSRISAIKQVASGRFGVTIEYLSHA
                     QEIQIKMAQGAKPGEGGQLPGHKVYPWIARVRYSTPGVGLISPPPHHDIYSIEDLAQL
                     IYDLKNANPAARINVKLVSEVGVGTIAAGVAKGHADVILISGHDGGTGASPVSSIKHA
                     GLPWELGLAETHQTLVLNNLRSRVRLETDGQLKTGRDVVIAALLGAEEFGFATGPLVA
                     MGCIMMRVCHLDTCPVGVATQNPELRRRFAGKPEDVVNFMRFIAEDVREHMAALGVRS
                     VDELVGRTDLLAVREELAHHKASKLDLSALLYRPDAGPEVGRTCTQAQAHGLEHSLDR
                     TTLLGLCAPALEAGAPVAATLPIRNIHRTVGTMLAGEIARRHGPEGLPEDTIQLTFQG
                     SAGQSFAAFLPPGVTFTLEGDANDYVAKGLSGGKLIIKPPAGTVFAPQDNIIIGNVAF
                     YGATGGEAYIRGAAGERFAVRNSGAVAVVEAIGDHGCEYMTGGRVAVLGPTGRNFAAG
                     MSGGIAYILDVDGSFAGRCNADMVDLLPLDDDDARELNNMVWKHAVYTGSSVAWRLVG
                     EWETARERFVKVL"
     gene            4914..6317
                     /locus_tag="PK829_02940"
     CDS             4914..6317
                     /locus_tag="PK829_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOU40203.1"
                     /translation="MKQNWRAWRRLANVAVVGVLLLVLTQTVFAQDAEPAVSAIDTVW
                     VLVCAFLVFFMQAGFGFLEAGFVRSKNVVNIMAENFMDTTFATVAFVLVGFGLMFGSG
                     NGFFGTEWFALRGIPEVFPGTTLPTLAFFFFQFAFCAAAGAIASGMMAERTNFVADLG
                     YSLITAGLIYPIFGHWVWGGGWLAQLGYIDFAGSSVVHIVGACIGLAGTLMLGKRRDK
                     VFGPSIRGHSIPLAALGTFVLWFGWFGFNPGSVLAAQPFKIASVVVTTDFGATTGAIS
                     AIFLAYFFTRKWDVSMAFNGALAGLVAITGSAAFVTPAAALVIGTIAGVIAYFGVQLM
                     EKLRIDDPVGAFPVHGLNGIFGILAVGIWGSDGLGLLSGGGFRQLGIQALGVVVCIAW
                     TLPLTLGFFYVVRRTIGLRVSAEVEATGLDFPQHGLESYPEFDGVELRGIQARDWEMD
                     IAPMPVERAKLVAGTGD"
     gene            6320..7378
                     /locus_tag="PK829_02945"
     CDS             6320..7378
                     /locus_tag="PK829_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40204.1"
                     /translation="MPRATFTYRHNTTEAAGHRQPALWAAIDLAGHGAMDRIEALPIY
                     RTARLLPDGRLAQRYQTLVAGVAIERASLEELETSIDHYLGLLVHYGRLPEYLFQLGD
                     RAWPIYRLEDQLTARFPGSRTFSAPTIGELRNELADHFKQVGVIDYRKGMTVLYLSRQ
                     DFQPDAPVCTLRAPGLAEMPIFRDQETVVHLVAPVNGRSIVTELAGGEGLLVLPGLVG
                     DYLADRGRLADSDSIGVRKLSAAAWAAAARGLHPYTCSFVYTDRSGDDRERREIPIFA
                     DEARGRLVAARTNRMGRVSLHFAADIPHLQFKVGEELTEIGKIPVPTYVTIDGIEEVV
                     HAEYVEARGSSTLAPLAL"
     gene            7560..8963
                     /gene="glnA"
                     /locus_tag="PK829_02950"
     CDS             7560..8963
                     /gene="glnA"
                     /locus_tag="PK829_02950"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOU40205.1"
                     /translation="MTPSDVQKLIEARGIEAVDVKFTDLFGQWQHFSLPIEEFSAEGA
                     FEEGLGFDGSSIRGFQSIESSDMILICDPNTAIVDPICAAPTLSLIGDVYDPITRQPY
                     SRDPRYVARKAGEYLKSTGIADTAYFGPEAEFFVFDKVMYSAGEYSAAYQVDSIEGPW
                     NSGIFGFGHSVPHKRGYFPVAPFDTLQDLRTEMVRELINAGVPIELHHHEVGTAGQCE
                     IDMRFDALLKMADNVQLYKYIVKNVAQRNGKTVTFMPKPIYADNGSGMHTHQSLWKDG
                     QPLFYDENGYAGLSQLAKWYIGGILKHINALLAICAPTTNSYRRLVPGYEAPVVVAYS
                     ARNRSAAVRIPMYSKSPKAKRIEFRCPDPAANPYLAFAACLMAGLDGIKNQIDPGDPA
                     EMDLFDEENINKVTMTVGKLNEALDALVADHAFLLEGGVFTQDVLDAYISYKHETEIG
                     PVSLRPHPYEFLLYYGV"
     gene            complement(9050..>10246)
                     /locus_tag="PK829_02955"
     CDS             complement(9050..>10246)
                     /locus_tag="PK829_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOU40206.1"
                     /translation="GLLLAFSILGNGLLLGLWWRGRAAASVTPPTPPALTLDEPSATV
                     TPGAVAAAASPLASEATPTRPGPLVATRAATATATAPMRTTVPSPTPITAPATGAPAA
                     PTEEPTAAPSPTPTTEPTPTPTASPTIAAVGTDWLQFVNRFRASAGVPPLLENTAWSL
                     EAFDHSRYMAYTGDLSHSQDVNSPYFSRAGMAAAENGNIAAGYSSSEPFQWAFNYWMS
                     APFHALPIIDPQLATTGFAEYRSPDALLGATATLDVRRGLGPLPPGVVYPIRYPGDGA
                     STWVLRYSLPEFPEALSTCPGYQQPTGAPIILQLGDGALTPHVTATSLAREGEYLAHC
                     WFDETTFTHPNEFRQRSARTILDQRDAIVIIPQEPLLLDATYAVRIDANGQTYTWSFH
                     TVAGPP"
BASE COUNT         1618 a         3358 c         3490 g         1780 t
ORIGIN      
        1 gcaagacctt gacgaaccgc tcgcgagcgg tctcccactc acccacaaga cgccaggcca
       61 cgctgctgcc ggtgtagacg gcgtgcttcc agaccatgtt gttgagttcg cgggcatcgt
      121 cgtcgtccag gggcagcaag tcaaccatgt cggcgttgca gcggccggcg aagctgccgt
      181 cgacgtccag gatataggcg atgccgccgg acatgcccgc ggcgaagttg cgccccgttg
      241 gccccagcac agccacccgg ccgccggtca tgtactcgca gccgtggtcg ccgatggcct
      301 cgacgacagc caccgcgccg ctgttgcgca cggcgaagcg ctcgccggcc gcgccgcgga
      361 tgtaggcctc gccgccggta gcgccgtaga aggcgacgtt gccgatgatg atgttgtcct
      421 ggggcgcgaa gaccgtgccc gccggcggct tgatgatgag cttgccgccc gacagcccct
      481 tggccacgta gtcgttggcg tcgccctcca gcgtgaaggt gacgcccggt ggcaggaagg
      541 cggcgaagct ctgcccggcc gatccctgaa acgtcagctg gatggtgtcc tccggcagcc
      601 cctccgggcc gtggcggcgg gcgatctcgc cggccagcat cgtgcccacg gtgcggtgga
      661 tgttgcggat gggcagcgtg gccgcgacgg gcgcgccggc ctccagcgcc ggggcgcaca
      721 ggccgagcag cgtcgtccgg tcgaggctgt gctccaggcc gtgggcctgg gcctgcgtac
      781 aggtacggcc aacctccggc cccgcgtctg gccggtagag tagcgccgac aggtcgagtt
      841 tgctggcttt gtggtgggcc aactcctcgc ggacggccag caggtcggtg cggccgacca
      901 gctcatcgac cgagcgcacg cccaacgcgg ccatgtgctc gcgcacgtct tcggcgatga
      961 agcgcatgaa gttgaccaca tcctccggct tgccggcgaa gcggcggcga agctccgggt
     1021 tctgcgtggc cacgccgacc gggcaggtgt ccaggtggca gacgcgcatc ataatgcagc
     1081 ccatggccac cagcgggccg gtggcgaaac cgaactcctc cgcccccagc agcgcggcga
     1141 tgaccacgtc gcggccggtc ttgagctggc cgtccgtctc caggcgcacg cgactgcgca
     1201 gattgttgag caccagcgtc tgatgcgtct cggccaaccc cagttcccac ggcagcccgg
     1261 cgtgtttgat gctcgacacc ggcgacgcgc ctgtgccgcc gtcgtggccg ctgataagaa
     1321 tgacgtcggc gtgccccttg gccacacccg cggcaatcgt gccgacgcca acctcggaca
     1381 caagcttgac gttgatgcgc gccgccgggt tggcgttctt caggtcgtag atgagctggg
     1441 ccaggtcttc gatggagtaa atgtcgtggt ggggcggcgg cgaaatgagg ccgacgccgg
     1501 gcgtcgagta gcgcacgcgg gcgatccacg ggtaaacctt gtggccgggc aactggccgc
     1561 cctcgccggg cttggccccc tgggccatct tgatctgaat ctcctgggcg tggctgaggt
     1621 actcgatggt gacgccgaag cggccgctgg ccacctgctt gatggcgctt atgcgcgagt
     1681 cgccgtcagg gccgggggca tagcgggccg ggtcttcgcc gccctcgccg gtgttgctct
     1741 tgccgccgac gcggttcatg gcaatggcca gtgtctcatg ggcttcgcgg ctgatggagc
     1801 cataggacat ggcccccgtc ttgaagcggc tcaggatggc ctccaccggt tcgacctcgt
     1861 ccagcggcac gggcgtgggc gcgaactgga actccagcag cgcccgcagc gtggccaggt
     1921 gcgtctcgcg ggcgtgaact tcgtcggcat actgcttata agcgcgatag tcgccgctgc
     1981 gaacggcatg ttgcagcttg tggatggtgg acggattgaa caagtggaac tcgccgccgc
     2041 tacggtactg gtagcgcccg ccgacctcta acgtgtggcc attggcggga cgtgccgggt
     2101 aagccgcggc gtggcgctgc gccgcctcgg cggccagcac gtccatgtca gccccctcga
     2161 tgcgcgtcgg tgtgccgctg aagtagcgat cgacgacggc gcggctgagg cccagcgcct
     2221 cgaagatctg cgccccgtgg tagctctgca ccgtgctgat gcccatcttg gagatggtct
     2281 tgaccacgcc cttgaccgcg gccttcaggt acttggccgc ggcggtgtcg tactccacat
     2341 ccagcagccg ccgggcgatc aggtcgtgaa gcgtctcgaa ggccaggtag ggattgactg
     2401 cgtcggcccc gtagccgacc aggcaggcgt agtggtggac ttcgcgcggc tcgcccgact
     2461 cgaccagcag gctgacccgc gtgcgcgtgc cggcggcgat gaggtgctcg tgcagcgcgg
     2521 agacggccag tagtgccggg ataggcgcgc gcgcccggcc gacgccccga tccgatagga
     2581 tgagcaggtt cgcgccgccg gccacggcct tgccggccgc ggcgcatagc tcggccagcg
     2641 ccgccgtcag cgccgcgccg tcgcccccgg ccgggtagag gatcggcaac gtcgccgccc
     2701 ggaagccggg cacgcgaatg gcgcgcagac tggccagcgc ggcattggtc agcagcggcg
     2761 acgggatgcg gatgcggcag cagtcttctg gccgcgggtt cagcaagtcc ccctccgcgc
     2821 cgagcagcat cgtgctgctg gtcaccagtt cctcgcggat ggcgtcaatg ggcgggttgg
     2881 tgacctgggc gaagagctgg cggaagtagt tgtagagcaa ttgcggccgc tccgacagca
     2941 cggccaccgg cgtgtcgtcg cccatcgagc cgagcggttc gacgccgtcg cgggccattg
     3001 gggccatgat caggcgcagg tcttcgaagg tatagccaaa ggcgtgctgg cggatggtca
     3061 cggtgtcgtg gtcgggcacg ggttcgtcgc cggccacggg caatgcctcc agcggcagca
     3121 ggtgctcgtc cagccagtcg ccatagggtc gggccgcggc gatggtctcc tttagctcct
     3181 cgtcggcgac gatgcggccg gcggcggtgt ctaccagcag catgcgcccc ggctgcaagc
     3241 gtcccttgtg cagcacctgc tccggcggga tgtcgagcac gcccacctcc gaggccatga
     3301 tgacccggtc gtcggcggtg acagtgtagc gcgccggccg cagcccgttg cggtcgagca
     3361 ccgcgccgac gagcgccccg tcgctgaaga cgatggccgc cgggccgtcc cacggctcca
     3421 tgagggcgct gtgataggcg tagaaggcgc gtttggccgc gctcatgccg gcgtgtttct
     3481 cccacggttc ggggatcatc atcatgagcg cctccggcag agagcggccg gccagcacca
     3541 ggaactccag ggcattgtcg aacatggctg agtcggtgcc gtcctcgtcg atgacgggca
     3601 gcacgcgggc caggtcgggg ccgaaagcgt ccgagcggaa gagcgcctgc cgcgccttga
     3661 tggcgttgac attgccgcga atggtgttga tctcgccgtt gtgggccagg atgcggttgg
     3721 gatgggcgcg cttccacgcc ggaaacgtgt tggtcgagaa gcgcgagtgg acgagagcga
     3781 tagccgaatc gaagcgcggg tcggacaggt cgggaaagta gtcgcgcagt tgctcggcca
     3841 ggagcatgcc cttatagacc agcgtccggc tggacaggct aacgacgtag aagacctgcg
     3901 cgccgccggc cggcgagtcg atggcccgct cggccagccg gcgcaggaca aagagcgccc
     3961 gctcgaaggc caacgcgtcg ccggccgcgc cggtggctgg gccgcggcca atgaacacct
     4021 ggcgcacaac cggctcactg gccaccgccg tggctcccag ggcgcggttg tcggttggca
     4081 cgtcgcgcca gcccagcagc gtgtgcccct cggcggcgac gaggccggcc agttgccgct
     4141 cgcaggccag ccgccgcgcc gggtcgcgcg gcaggaagag catgccgacg ccgtactcgc
     4201 ccgccggtgg caggtgaaat ccgaggcgag cggcctcggc ggcgaaaaag gcgtggggaa
     4261 gttgggtgag gatgccggcc ccgtcgccgg tgttcggctc cgagccgcgc gcgccgcggt
     4321 ggctgaggtt gtccagaacc gtcagcgcgt cggcgacgat ggcgtgcgac cgctggccct
     4381 tgacgtgggc gacgaagccc gtgccacagg catcatgctc ataacgagga tcgtacaagc
     4441 cctgctgggc aggcccttcg ccggaggcta cgtctcgctc cggcggttgt tgtccacttt
     4501 tgcgcctctg ggaacgcccc atcttcttct cctcgtagtc ctcacagtcg ccttgcggcg
     4561 agctgggcat tcacggccaa cgccgcgcgg ccggctctta atgtgcaaaa tgcgtcacct
     4621 tctcaggcga cgccgtctat tcactccata gtgtatctcg cgatcacccg gccttcctga
     4681 ggcactttgc acgaaacaac actctgaact ttatgatctt tagacgtttt gctgcggcgc
     4741 gtcatcctgt atgattcagg cttgtgatga gtggcggcca cgggctgcca acaacagaat
     4801 cattgttttg ggccaggacg cctcgcaccc cgccacgtgg gttgacgagg cgtttttgcg
     4861 ttcatcagcg ggatgaacga cagcggccaa cagtcatgca gaggaggaac atgatgaagc
     4921 agaattggcg agcgtggcgg cgactggcaa atgttgctgt ggtgggtgtg ctcttgctgg
     4981 tgctgacgca aacggtcttc gcgcaggacg cggaaccggc cgtcagcgcg atcgatacgg
     5041 tctgggtgct ggtctgcgcc ttcctggtgt ttttcatgca ggcgggcttc ggcttcctgg
     5101 aggcgggctt cgtccgctcc aaaaatgtcg tcaacatcat ggccgagaac ttcatggaca
     5161 cgacgttcgc cacggtggcc ttcgtccttg tcggcttcgg gctgatgttc ggctccggca
     5221 atggcttctt cggcacagag tggttcgccc tgcgcggcat tcccgaagtc ttccccggca
     5281 cgacgctgcc gacgctggcc ttcttcttct tccagttcgc cttctgcgcc gccgccggag
     5341 ccatcgcctc cgggatgatg gccgagcgca ccaacttcgt cgccgacctg ggctacagcc
     5401 tgatcaccgc cggcctcatc taccccatct tcggccattg ggtttggggc ggcggttggc
     5461 tggcgcagtt gggctatatc gacttcgccg gttcgtcggt tgtccacatc gtcggcgcgt
     5521 gcatcggcct ggccggcacg ctcatgctcg gcaagcggcg cgacaaggtc tttggcccca
     5581 gcatccgcgg ccacagcatt cccctggcgg cgctgggcac atttgtcctg tggttcggtt
     5641 ggtttggctt taaccccggc agcgtcctcg ccgcccagcc cttcaagatc gccagcgtcg
     5701 tcgtgacgac cgactttggc gcgaccaccg gagccatcag cgccatcttc ctggcctact
     5761 tcttcactcg caagtgggac gtgagcatgg ccttcaatgg cgcactggcc ggcctggtgg
     5821 ccatcaccgg ctcggccgcc ttcgtcacgc cggccgcggc cctggtcatc ggcacaattg
     5881 ccggcgtcat cgcctacttt ggcgtgcagt tgatggaaaa gctgcgcatt gatgaccccg
     5941 ttggcgcgtt cccggtgcat ggcctgaatg gcatctttgg catcctggcc gtgggcatct
     6001 ggggcagcga tgggctgggc ttgctcagcg gcggcggctt tcggcaattg ggcattcagg
     6061 cgttgggcgt cgtcgtttgc atcgcctgga cgttgccgct gacgctgggc ttcttctacg
     6121 tggtgcgccg cacaatcggc ctgcgcgtct cggccgaggt cgaggccacg gggctggact
     6181 tcccgcagca cggcctggaa tcgtacccgg agttcgacgg cgtcgaactg cgtgggattc
     6241 aggcgcgcga ttgggagatg gacatcgcgc cgatgccggt cgagcgggct aaactggtcg
     6301 ccgggacggg ggattagcca tgccgcgcgc gacatttacc tatcgccata acacgaccga
     6361 ggccgccggc catcgtcagc cggccctctg ggcggccatt gacctggccg ggcacggcgc
     6421 gatggaccgg attgaggcgc tgccgatcta ccggacggcc agactgctgc ccgacggccg
     6481 gttggcccag cgctaccaga cgctcgtcgc cggcgtcgcc atcgagcgag ccagcctgga
     6541 ggaactggaa acgtcgatcg atcactatct gggcttgttg gtccactacg gtcgcctacc
     6601 ggaatatctc tttcagctcg gcgaccgcgc ctggcccatc tatcggctgg aagaccagct
     6661 gacggcgcgc tttcccggca gccggacgtt cagcgcaccg accatcggcg agctgcgcaa
     6721 cgagttggcc gaccacttca agcaggtggg cgtcatcgac taccgcaagg gcatgacggt
     6781 gctctatcta tcgcgccagg acttccagcc ggacgcaccg gtttgcaccc tgcgcgcgcc
     6841 gggtctggcc gagatgccca tcttccgcga ccaagagacg gtcgtccacc tggtcgcgcc
     6901 agtcaacggc cgctcaatcg tcaccgagct ggccggtggc gaggggttat tggtgctgcc
     6961 ggggctggtg ggcgactatc tggccgaccg cggccggctg gccgacagcg acagcatcgg
     7021 cgtgcgcaag ctatcggccg cggcctgggc cgccgcggcg cgcggcctgc acccctatac
     7081 gtgcagcttc gtctacaccg atcgctctgg cgacgaccgg gagcggcgcg agatccccat
     7141 cttcgccgac gaggcccgcg gccggctagt cgccgcccgc accaaccgga tggggcgcgt
     7201 ctcgctgcac tttgccgccg acatccccca cctgcaattc aaagtgggcg aagaactgac
     7261 cgagatcggc aaaatccctg tgccgaccta cgtcaccata gatggcatcg aggaggtcgt
     7321 tcacgccgag tatgtggagg cgcgcgggag cagcactctg gccccacttg ctctataatg
     7381 acagtgtccg cgcccaagcg acgagtcgga gccactgcca tctgtcttga tctattgttg
     7441 agttgacgcc ggacccggcc aaatccagcc cacctgggcg gccggggccg gcgcaaagtc
     7501 gcgattcggg agaatcgcgc cacgtttgat tgtctaacaa ttgcggagga acaagaacca
     7561 tgacacctag cgatgtacag aaactgattg aggctcgcgg catcgaagcc gtcgatgtga
     7621 agttcaccga cctgttcggg cagtggcagc acttctcctt gcccatcgaa gagttcagcg
     7681 ctgaaggcgc tttcgaagag gggttgggct tcgatggctc ctccatccgt ggcttccaga
     7741 gcattgagtc gagcgacatg atcctgatct gcgacccgaa cacggccatc gtcgatccca
     7801 tctgcgccgc gccgacgttg agcctcatcg gcgacgtgta cgaccccatc actcgccagc
     7861 cgtattcgcg cgacccgcgc tacgtggccc gcaaggcggg cgagtatctg aagagtaccg
     7921 gcatcgccga cacggcctac ttcggcccgg aggcggagtt cttcgtcttc gacaaggtga
     7981 tgtattcggc cggcgagtac tccgcggcct accaggtgga cagcatcgag gggccgtgga
     8041 attcgggcat cttcggcttt ggccactccg tgcctcacaa gcgcggctat ttccccgtgg
     8101 ccccgttcga cacgctgcag gatctgcgca ccgaaatggt gcgcgagctg atcaacgccg
     8161 gcgtgcccat cgagttgcat caccacgaag tcggcacggc cggccagtgc gagatcgaca
     8221 tgcgtttcga cgcgctgctg aagatggccg acaacgtgca gctctacaag tacatcgtca
     8281 agaacgtggc ccagcgcaat ggcaagacgg tcaccttcat gcccaagccc atctacgccg
     8341 acaacggctc cggcatgcac acccaccaga gtctgtggaa ggacggccag ccgctgttct
     8401 acgacgagaa cggctacgcc ggcctgagcc aactggccaa gtggtacatt ggcggcatcc
     8461 tgaagcacat taacgccctg ctggccatct gcgcgccgac gaccaactcc taccgccgcc
     8521 tggtgcccgg ctacgaggcc cccgtcgtcg tcgcctactc ggcccgcaac cgttcggcgg
     8581 ccgtgcgcat ccccatgtat tccaaatcgc ccaaggccaa gcgcatcgag ttccgctgcc
     8641 ccgacccggc ggccaacccc tacctggcct tcgccgcctg cctcatggcc ggtctggacg
     8701 gcatcaagaa ccagatcgac cccggcgacc cggccgagat ggatctgttt gatgaggaga
     8761 acatcaacaa ggtgacgatg accgttggca aactcaacga agccctcgac gcgctcgtgg
     8821 ccgaccacgc cttcctgctg gagggcggcg tcttcactca ggatgtgctg gacgcctaca
     8881 tcagctacaa gcacgagacg gagatcggcc cggtcagcct gcggccgcat ccctacgagt
     8941 ttttgctcta ctacggcgtc taagcaaatc aggggctagg ggctaggttc tagggggtag
     9001 ggaagagcgc tcttccctac cccctagccc ctagccccta cccccttcct caaggcgggc
     9061 cggcgactgt gtggaagctc caggtgtagg tctgcccgtt ggcgtcgatg cgcaccgcgt
     9121 aggtcgcgtc gagcagcagc ggttcctgcg ggatgatcac gatggcgtcg cgctgatcga
     9181 ggattgtccg cgccgagcgc tgccggaatt cgttggggtg ggtgaatgtg gtctcatcga
     9241 accagcagtg ggccaggtat tccccctcgc gggccagcga ggtcgccgtg acgtggggcg
     9301 tcagagcgcc gtcacccagt tgcaggatga tcggcgcgcc ggttggctgc tggtagccgg
     9361 ggcaagtgct gagcgcctcc ggaaactccg gcagcgagta gcgcagcacc cacgtgctcg
     9421 cgccgtcacc agggtagcgg atgggataaa ccacgccagg cggcagcggc cccagtccgc
     9481 ggcgcacgtc cagcgtcgct gtcgccccca gcagggcgtc cggcgagcgg tattcggcga
     9541 agccggtcgt agccagctgt gggtcgatga tgggcagggc gtggaaggga gccgacatcc
     9601 agtagttgaa agcccactgg aacggttctg agctactgta gccggcggcg atgttgccgt
     9661 tttcggccgc ggccattccg gcgcggctga agtacggact gttgacatcc tggctgtggc
     9721 tcaggtcgcc agtgtaggcc atgtagcggc tgtggtcgaa ggcctccagc gaccaggccg
     9781 tattctccag cagcggcggc acgccggccg acgcgcgaaa gcgattgacg aattgcagcc
     9841 agtccgtgcc cacagccgcg atggtcggcg aggccgttgg cgtcggggtc ggctcggttg
     9901 tgggtgttgg cgatggggct gcggttggtt cctcggttgg cgcggccggc gcgccggttg
     9961 ccggggcggt gataggcgtg ggcgagggga cagttgtacg catgggcgct gtggctgtcg
    10021 ccgtagcggc gcgcgtggcc acgagcgggc cagggcgggt gggggtggct tcgctggcca
    10081 gtggcgaagc agccgcggca acagcgcccg gagtaactgt cgccgatggc tcgtccagcg
    10141 tcaatgccgg gggcgtgggt ggcgtcactg acgcggccgc ccgtccgcgc caccataacc
    10201 ccagcaatag cccattcccc agaatcgaga aggccagcaa tagccc
//
LOCUS       DAOSMQ010000051        10146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6618_length_10146_cov_5.631256, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000051 DAOSMQ010000000
VERSION     DAOSMQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_10146_cov_5.631256"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK829_02960"
     CDS             <1..258
                     /locus_tag="PK829_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40207.1"
                     /translation="SVRLAAYEFLLTLYYPDKNREALLQLLQNMLSDSEDAIRAAGAR
                     YADQADVAAELRSFLLRWQKTAPGQGWANTESFELVGRLLN"
     gene            277..1266
                     /locus_tag="PK829_02965"
     CDS             277..1266
                     /locus_tag="PK829_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40208.1"
                     /translation="MLLKPAYKLTIGDKIVDTTDEPQASTVVALVVDLDLAAPADSFT
                     LTLGQVGGFQPRRDDDAKIELGFEDELTQVMAGKVVTADNGLTTRRVIGHGAARLLLA
                     ATATETFLSLTAGQIVERLAERAAVPVERAEPGITFPAYVIDGRRNLYRHLHDLADLC
                     GFDLYINSAGKLVFAPFRSGNAIHIFEFGKQIIELEMEQRPPLAGQVQVFGESAGGGQ
                     GDNAWAWLTKDFSGLKGTGGDAQPVLLLEQAVARTGEAAQAVAEAAFTRLTRQRLRGT
                     LLSTGQPKVKLGDAIELRGLPDTAANGTFQVRRVRHTIDKTRGFTTRIGFRSI"
     gene            1280..2083
                     /locus_tag="PK829_02970"
     CDS             1280..2083
                     /locus_tag="PK829_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HOU40209.1"
                     /translation="MTTVVDTLQQIIRHELRSLHIAELGLVEAVFPHSDGGDDDNYAC
                     DVRLKDSGLLLRRVPVMTGHIGTVAIPNVGDLVLLNFEQGDVNHALIVGRFYNNIDRP
                     PLSNPNEIIFRLPLAEADDKSILAAVRNLPANAEPREIIVEMPPQITVRISDGAVRAT
                     AGDTEMILDQPHNSGGRVTVVTGRTKIVMNQDGDVRVDVAGSLTVKADRDLTLEANGD
                     VAIKGMNVKAEAAMNAEVKGGLAAKVQGDLSATVQGLNLSLKGMATFSP"
     assembly_gap    2238..2337
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2435..2731
                     /locus_tag="PK829_02975"
     CDS             2435..2731
                     /locus_tag="PK829_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40210.1"
                     /translation="MITPGASGPPDMGTIVAVFPPVITANGLPLATTGSLCMMINSVW
                     GFPYPLVIGPLASSGVQVAGRALVRTFDRIPTPPGILLILGPPAAPFITDQWPP"
     gene            2772..3224
                     /locus_tag="PK829_02980"
     CDS             2772..3224
                     /locus_tag="PK829_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOU40211.1"
                     /translation="MSDNTRWGADLRLLDNLLQQNDLDVGKDLFVTERLETGQSDLET
                     VVADDNLKQALLLRFLTRRGELEPLGHPTYGSRLFDLLGERNTLANRNRAKLYVLEAL
                     AMEPRVAEVLSVQVETNHAEPTQIDISVRLRPIYADTVLNLVFPFFLE"
     gene            3279..4880
                     /locus_tag="PK829_02985"
     CDS             3279..4880
                     /locus_tag="PK829_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HOU40212.1"
                     /translation="MNQPTVTLIQRPYQEIVDDVLTAIVGGVVNEPIRYDLKRDLYPL
                     SEPADDIRGVTGLHEGKAYAFLKEIDYVFNEGSNAVEWDENGTRPDDDTDFRVDYFRP
                     NSRSPLTDINVGSVTRTLSEAVSREINTVYEQVNLAYLSAFIDTATGRSLELVVAILG
                     ITRKTKEFAVGLETFFRQAGAAGNITIPEGTILATTKGDVIFETTQPRTLQQGQVRID
                     APIRATIDFKGEKGKVTAAGAISQLLQPIVGITRVTNFDGTFLGTDDETDEELRLRAK
                     AALRSLGKATLAALQRVVLEGGGKLTEVWDPDGAPDHRSDPGSVTLLIEAEPERFASL
                     NTAVQETRAAGVQALVISRYVFFKPRILATIAPGLTAAGKLQVVDELIAAVQDYTDGL
                     SSGQPAEGQAMLKALKDVEDVVDVKIVDVLVWRSDLGRPAQATLVDGIANAVVASRAG
                     GAPDDLDALKLIITSTLQETPPLVPTNTRIPDRSLLQGPDGQPATDAQIESGDFRVVA
                     TVGGDQWWVVLDMEPADVVVEEKVS"
     gene            4881..>10146
                     /locus_tag="PK829_02990"
     CDS             4881..>10146
                     /locus_tag="PK829_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40213.1"
                     /translation="MANKTDRIISYLPSTFRKQPRGVTLHAVVDAYGRELQDAENTLA
                     EVTQSHWVDYADRLQPRIDDLARIAALYGLGPRPDETVEAFREHLKRYIRTYLDGTVT
                     VQGVLRIAAQALGLDIADDYDALDTWWTRPQGDAITVAAPDGRDAAERVLGARAAFNR
                     GLSSLPAQVRGTADLSLGADCRENRWLRLVVDGAGPFEIDVSAGAADLSAVPGQLIAG
                     NINAEIEQPIASFDGHALTLTSLRRGFASVIEVRDILNDAAEAVLGLPPRAYNGRDES
                     PARVRGLVDLSGVLDLSEARYLRVLIDGARLAEIDVAGPDPAGTLLHQVVERLNAVLG
                     EGTVTADDKFLTLTSPTAGLGSSIVFQQAAAQQALLRLFGPISQTHLGRGTLSAQVVG
                     RRDLSGGVDLSRRARLLLRVDGAEHAIDCAGRVPENTQLPELVAAINDALGARVATHN
                     GGKITLTSTASGLSSEIVFLTPDEEDATELLFGIRPRVFHGRAAAAGRVLGTPDLSQG
                     VDLRSRYRLRLRLDGRPPVTVDLRSHAGADNLSAVSPQALADAIDAQMGADIASTDGA
                     RLNLVSRSEGSASSIEVVPLAASQMERFITRAFVTGEAADALFGFNRKSEPGTAATNA
                     RVSGGVDVHRGVDLRQNRYLRLAIDDQPAVEIDAAGVRPRATLIDELVNAINLGIAQA
                     QTPVVATFADGHLTLTSPTHGAAGRLVLASAGSDDALGRLLGLPPGETRGQDATPARL
                     VGAVDLSAGVDLSAGDRIKLSIDGGQPVEIACAEGATDPARVRLSTIRSAINRALRRK
                     LASHDGKFLSVTSRRTGSSSRIVLDSPDTGDATAALFGVAVPAEFSGQDAVPAQFVGT
                     ADLSAGVDLSQESTLRLTVDGTPPLDIDCRGKDARLTRLDEIAARINRAVALALTPRI
                     ASHDGRRLILSSPTLGRASRIAFEPPRATDALPLLLGIAPAEFRGQNAESVRFVGTVA
                     LGPTVDLSAGDAVRLGLDGAEPVTVACAAGAADPAAVPPSLIVANLNAALGVNAASFD
                     GRFLQIASPTVGASSQLVFATPDVGDATAALFGIESPREYHGHDQTRAEVTGRQDWRN
                     GVDLVYLGIARYLRLGVDGRPPQVVDCRPAETAAPNFATLTEIAAAIDDALGANVADV
                     VERDGGQVLALRSPTTGQGSRLVVEAHSSGDARALLFGSVPDTTTGVDAQPAVITGEV
                     DLLAPADLSRRSVLRLAIDGGLPQDINVAGYAPGATLLEEVVTAINAALGTADGPAAV
                     AAATAEQRLQLTSPTTGPASRLELLPLRTIDLIEYPAETAALPPRTVRHNQSWPVDNR
                     GALAGMAEVLITAPSGVAGVVLVNETLGQQIRLLTAIGPRQAARIWADGARLRAEIRP
                     ASGPARPVPDRQILAGPPGAQARVPFPGEWGFSGRAGERPTLQLNNPTADGIVRLTAR
                     AATATGRLALRVADSDVPPATLDATAADGQTVALPGRVEATGDGPVYRLMGNGGEARA
                     ELRAGPGVEIERFVDRVVVAHGTIHAAATGALLIVSRLEALFRATLTLTTPAGEPVVE
                     EYARVTIGDDPAADNALARAIQTRPSALVRAESLAKIEALSLPLGRSRWRFLDCYAPR
                     FNSARFDRARFPGEVCHERGVFDASRFENAPPEPVAAAFTGIDDPPDAGVQVQFTTAR
                     YQPGAFRVQVPADLPEVFGGRFNQARFGQGKNRPELYPFVVTEPPGDENHVEKVFDHS
                     RLVEVEIVPRVPLGWEPVAMPFREPRTLT"
BASE COUNT         1641 a         3652 c         3353 g         1400 t
ORIGIN      
        1 agcgtgcggc tggcggcgta tgaattcctg ctgacgctct actacccgga caaaaaccgc
       61 gaggcgctgc tgcaactgtt acagaatatg ctgtccgaca gtgaagatgc catccgggcc
      121 gccggcgcgc gttatgccga ccaggccgac gttgccgccg agttgcgcag cttccttcta
      181 cgctggcaga agacagcccc gggccagggg tgggcgaata cagagagctt tgagttggtt
      241 ggccgccttc tcaattgatc aagcgcctgg tgcgacatgc ttctgaaacc cgcctacaaa
      301 ctcaccatcg gcgacaagat cgtcgatacc accgacgagc cgcaggccag cacggtcgtg
      361 gccctggtgg tcgatctcga cctggccgcg ccggccgaca gcttcacgct gaccctcggc
      421 caggtcggcg gcttccagcc gcggcgcgac gacgacgcca agatcgagtt gggttttgag
      481 gacgaactga cgcaggtgat ggccggcaag gtcgtgaccg ccgacaacgg cctgaccacc
      541 cgccgcgtca tcggccacgg cgcggcccgg ctgctgctgg ccgccaccgc gaccgagacc
      601 ttcctgagcc tgaccgccgg ccagatcgtc gaacgcctgg ccgagcgcgc cgccgtgccc
      661 gtcgagcggg ccgagccggg catcactttc ccggcctacg tcatcgacgg ccggcgcaat
      721 ctctaccgcc acctgcatga cctggccgac ttgtgcggct tcgacctgta catcaacagc
      781 gccggcaagc tcgtcttcgc tcccttccgc agcggcaacg ccatccacat cttcgagttc
      841 ggcaaacaga tcatcgagct ggagatggag cagcgcccac cgctggccgg gcaggtgcag
      901 gtctttggcg agagcgccgg cggcgggcag ggcgacaacg cctgggcctg gctgacgaag
      961 gacttcagcg gcctgaaggg cacgggcggc gacgcccagc cggtcttgct gctggagcag
     1021 gccgtggccc gcaccggcga agcggcccag gccgtggccg aggccgcctt cacccggctg
     1081 acgcggcagc ggctgcgcgg cacgctgctg agcaccggcc agcccaaagt caaactgggc
     1141 gacgccatcg agttgcgcgg cctgcccgac acggccgcca acggcacgtt ccaggtgcgc
     1201 cgcgtgcgcc acaccatcga caagacgcgc ggcttcacga cgcgcatcgg cttccgcagc
     1261 atctaaaggg gcgaaagaca tgaccaccgt tgtcgatacc ctgcaacaga tcatccgtca
     1321 cgagctacgc tcgctccaca tcgccgaact gggcctggtg gaggccgtct tcccccacag
     1381 cgacggcggc gacgatgaca attacgcctg cgacgtgcgg ctgaaggaca gcggcctgct
     1441 gctgcggcgc gttccggtga tgaccggcca catcggcacc gtggccatcc ccaacgtggg
     1501 cgatctggtg ctgctcaact tcgagcaggg cgacgtcaat cacgccctca tcgtcggccg
     1561 cttctacaac aacatcgacc ggccgccgct gagcaatccc aatgagatca tcttccgcct
     1621 gccgctggcc gaggccgacg acaagagcat cctggccgcc gtccgcaacc tgccggccaa
     1681 cgccgagccg cgcgaaatca tcgtcgagat gccgccgcag atcaccgtgc gcatcagcga
     1741 cggcgcggtg cgggccacgg ccggcgacac cgaaatgatc ctcgaccagc cccacaacag
     1801 cggcggccgg gtcacggtcg tcaccggccg gacgaagatc gtcatgaacc aggacggcga
     1861 cgtgcgcgtg gacgtggccg gcagcctgac cgtcaaggcc gaccgcgacc tgacgctgga
     1921 ggccaatggc gacgtggcca tcaagggcat gaacgtcaag gcggaggccg caatgaacgc
     1981 cgaggtcaag ggcggtctgg cggccaaggt gcagggcgat ctctcggcca cggtgcaggg
     2041 cttgaacctg tccttgaaag gcatggccac gttctccccc tagaggtgtc gggcgatttg
     2101 gcaaatcgcc ccacaaaagg agttgcaacg tagggcgatt tggcaaatcg ccccacaaaa
     2161 gcagttgcga cgtagggcga tttggcaaat cgccccacaa aaggagttgc gacgtagggc
     2221 gatttggcaa atcgcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca
     2341 aaaggagttg cgacgtaggg cgatttggca aatcgcccca caaagtagga gtcatgagat
     2401 gccaggatcg ccagtttcta taggagcaag cgtgatgatc accccgggcg cttccgggcc
     2461 gcccgacatg ggcacaattg tggccgtctt cccgccggtc atcaccgcca acggcctgcc
     2521 gctggccacg accggctcgc tgtgcatgat gatcaactcg gtctggggct tcccctaccc
     2581 gctggtcatc ggcccgctgg ccagcagcgg cgtgcaggtc gccggccggg cgctggtgcg
     2641 caccttcgac cgcattccga cgccgccggg catcttgctc atcctggggc cgccggcggc
     2701 gccattcatc accgaccagt ggccgccgta ggaatctgga atgtggaatt aagaattagg
     2761 aatcgaagaa catgagcgac aacacccgct ggggcgcaga cttgcgcttg ctggacaacc
     2821 tgctacagca gaacgatctc gacgtgggca aagacctctt cgtcaccgag cggctggaga
     2881 cggggcagag cgacctggaa acggtcgtgg ccgacgacaa ccttaagcag gccctcctgc
     2941 tgcgctttct gacgcggcgc ggcgaactgg agccgctggg gcatccgacc tacggctcgc
     3001 gcctgttcga cctgctgggc gagcgcaaca cgctggccaa ccgcaaccgg gccaagctct
     3061 acgtcctgga ggcgctggcg atggagccgc gcgtggccga agtcttgagc gtgcaggtcg
     3121 agaccaatca cgccgagccg acgcagatcg acatcagcgt ccgcctgcgc cctatctatg
     3181 ccgatactgt cctgaatctc gtatttccct ttttcctgga gtaggcggcg tctctcacgc
     3241 gggagaagtc gggaaaaagc cggaaggaga ctagacgcat gaatcagcca accgtaacac
     3301 tcatccagcg gccctatcag gagatcgtcg atgacgtgct gaccgccatc gtcggcggcg
     3361 tggtcaacga gccgatccgc tacgatctca agcgcgatct ctacccgctg tcggagccgg
     3421 ccgacgacat tcgcggcgtc accggcctcc acgagggcaa ggcctacgcc ttcctgaagg
     3481 agatcgacta cgtcttcaac gagggcagca acgccgtcga gtgggacgag aacggcacac
     3541 ggcccgacga cgacaccgat ttccgcgtcg attacttccg gcccaacagc cgctcgcccc
     3601 tgaccgacat caacgtcggc agcgtcacgc gcaccctcag cgaggccgtc agtcgcgaga
     3661 tcaacaccgt ctacgagcag gtcaacctgg cctacctgtc ggcgttcatc gacacggcca
     3721 cggggcgctc gctggagctg gtcgtggcca tcctgggcat cacccgcaag accaaggagt
     3781 tcgccgtcgg cctggagacc ttcttccgcc aggccggggc cgccggcaac atcaccatcc
     3841 ccgaagggac gatcctggcg acgaccaagg gcgacgtcat cttcgagacg acgcagccgc
     3901 gcacgctgca acagggccag gtgcgcatcg acgcgcccat ccgcgccacc atcgatttca
     3961 agggcgagaa gggcaaggtc accgccgccg gagccatcag ccagctcttg cagcctatcg
     4021 tcggcattac ccgcgtgacc aacttcgacg gcaccttcct gggcacggac gacgagaccg
     4081 acgaggaact gcggctgcgg gccaaggccg ccctgcgctc gctgggcaag gccacgctgg
     4141 ccgccctgca acgtgtcgtc ctggaaggcg gcggcaagtt gactgaggtc tgggaccccg
     4201 acggcgcgcc cgatcatcgc tccgaccccg gcagtgtgac cctgctcatc gaggccgagc
     4261 cggagcgctt tgccagcctc aacaccgccg tccaggagac gcgcgccgcc ggcgtccagg
     4321 cgctggtcat cagccgctac gtgttcttca agccgcgcat cctggccact atcgccccgg
     4381 gcctgaccgc cgccggcaag ctgcaggtcg tcgatgaact catcgccgcc gtgcaggact
     4441 acaccgatgg cctgagcagt ggccaaccgg ccgagggcca ggccatgctg aaggccttga
     4501 aagatgtgga agatgtcgtg gacgtgaaga tcgtcgatgt cctggtctgg cgttctgacc
     4561 tcggccgccc ggcgcaggcg acgctggtgg acggcatcgc caatgccgtc gtcgccagcc
     4621 gggccggcgg cgcgccggac gacctcgacg cgctcaagct gatcatcacc agcacactcc
     4681 aggagacgcc gccgctcgtg cccaccaata cgcgcatccc cgaccgcagc ctgctgcaag
     4741 ggccggacgg ccagccggcc accgacgccc agatcgagag cggcgacttc cgcgtggtgg
     4801 ccaccgttgg cggcgatcag tggtgggtcg tgctcgacat ggagccggcc gacgtcgtcg
     4861 tggaggagaa agtgagctag gtggccaaca agaccgaccg cattatcagc tatctgccct
     4921 cgaccttccg caagcagccg cgcggcgtga cgctccacgc cgttgtcgat gcctatggcc
     4981 gcgagcttca ggacgctgag aacaccctgg ccgaggtcac gcagtcccat tgggtggact
     5041 acgccgaccg tctgcaaccg cgcatcgacg acctggcccg catcgccgcg ctctacggcc
     5101 ttggcccgcg gcccgacgag acggtcgagg ccttccgcga gcacctgaaa cgctacatcc
     5161 gcacctattt agacggcacg gtgacggtgc agggcgtcct gcgcatcgcc gcccaggcgc
     5221 tggggctgga catcgccgac gactacgacg cactcgacac gtggtggacg cgcccgcaag
     5281 gggatgccat caccgtggcc gcgcccgacg gccgcgacgc cgccgaacga gtgctgggcg
     5341 cgcgggcggc tttcaaccgc ggcctgtcga gcctgccggc gcaggtgcgc ggcacggccg
     5401 acctcagcct cggcgcggac tgccgcgaga atcgctggct gcgcctggtc gttgacggcg
     5461 cggggccgtt cgagatcgac gtcagcgccg gcgcggccga cctgagcgcc gtgcccggcc
     5521 aactcatcgc cggcaacatc aacgccgaga tcgagcagcc catcgccagc ttcgacggcc
     5581 acgcgctgac gctgacctcg ctgcggcgcg gcttcgccag cgtcatcgaa gtgcgcgaca
     5641 tcctcaacga cgccgccgag gctgtgctgg gcctgccgcc gcgggcctac aacggccgtg
     5701 acgagtcgcc ggcgcgtgtg cgcggcctgg tggatctcag cggcgtcctc gatctgtccg
     5761 aggcgcgcta cctgcgcgtg ctgatcgacg gcgcgcgcct ggccgagatc gacgtggctg
     5821 gccccgaccc ggccggcacg ctgctgcacc aggtcgtcga acggctgaat gccgtcctgg
     5881 gcgagggcac cgtgacggcc gacgacaagt tcctgacgct gacctcgccg acggccgggc
     5941 tgggcagcag catcgtcttc cagcaggccg cggcccagca ggcgttgctg cgcctcttcg
     6001 gccccatcag ccagacgcat ctcggccggg gcacgctgtc ggcgcaagtc gtcggccggc
     6061 gcgacctcag cggcggcgtt gatctgagcc ggcgcgcgcg cctgttgctg cgcgtcgatg
     6121 gcgcggaaca tgccatcgac tgcgccggcc gcgtgccgga gaatacccag ttgcccgaac
     6181 tggtcgccgc catcaacgac gccctgggcg cgcgggtagc cacccacaac ggcggcaaga
     6241 tcaccttgac ctcgaccgcc tccggcctga gcagcgagat cgtcttcctg acgcccgatg
     6301 aggaggacgc caccgaactg ctcttcggca ttcgcccgcg cgtcttccac ggccgggcgg
     6361 cggccgccgg ccgcgtcctg ggcacgcccg acctgagcca gggggttgac ctgcgctcgc
     6421 gctaccggct gcggctgcgg ctggacggcc gcccgccggt aacggtcgat ttacgcagcc
     6481 atgccggcgc ggacaacctg agcgccgtgt cgccgcaagc gctggccgac gccatcgacg
     6541 cgcagatggg ggccgacatc gccagcaccg acggcgcgcg gctgaacctc gtctcgcgca
     6601 gcgagggcag cgccagcagc atcgaggtcg ttcccctggc cgcgtcgcag atggagcgct
     6661 tcatcacccg cgccttcgtc actggcgagg ccgccgatgc gctctttggc ttcaaccgca
     6721 agtcggagcc gggcacggcg gcgaccaatg cccgtgtctc cggcggggtg gacgtccacc
     6781 gtggcgtcga tctgcgccag aaccgctatc tgcgcctggc catcgacgac caaccggcgg
     6841 tcgaaatcga cgccgctggc gtgcgcccgc gggccacgct gatcgacgaa ctggtcaacg
     6901 ccatcaacct cggcatcgct caggcccaga cgcccgtcgt ggccacattt gcggacggcc
     6961 acctgaccct gacctcgccc acccacggcg cggccggccg ccttgtactg gcatcagccg
     7021 gcagcgatga cgcgctgggc cggctgctgg gtctgccgcc cggcgagaca cgtggccagg
     7081 acgccacccc ggcccggctg gtgggcgcgg ttgacctgag cgccggcgtc gacctgagcg
     7141 ccggcgatcg catcaagctg agcatcgacg gcgggcagcc ggtggagatc gcctgcgccg
     7201 agggcgcgac cgacccggcg cgcgtccgtc tcagcaccat ccgcagcgcc atcaaccggg
     7261 cgctgcggcg caagctggcc agccacgacg gcaagttcct cagcgtcacc tcgcgccgga
     7321 cgggcagcag cagccggatc gtcctcgaca gccccgacac aggcgacgcc acggcggccc
     7381 tcttcggcgt cgccgtgccg gccgagttca gcggtcagga cgccgtgccg gcccagttcg
     7441 tgggcacagc tgacctcagc gccggggttg acctgagcca ggagagcacg ctgcggctga
     7501 ccgtggacgg cacgccgcca ctggacatcg actgtcgcgg caaggacgcg cgtctgacgc
     7561 ggctggacga gatcgccgcg cgcatcaatc gcgccgtcgc cctggccctg acgccgcgca
     7621 tcgccagcca cgacggccgg cggctcatcc tcagctcgcc caccctcggc cgggccagcc
     7681 gcatcgcctt cgagccaccg cgcgccaccg acgcgctgcc gctgctgctg ggcatcgccc
     7741 cggctgagtt ccgcggccag aacgccgaga gcgtgcgctt cgtgggcacg gtcgcgcttg
     7801 gccccacggt cgatctgagt gccggcgacg ccgtgcgcct cggcctggac ggcgcggagc
     7861 cggtgacggt cgcctgcgcg gcgggggcgg ccgacccggc ggccgtgccg ccaagcctga
     7921 tcgtcgccaa tctcaacgcg gccctgggtg tcaacgcggc cagcttcgac ggccgcttcc
     7981 tgcaaatcgc ctcgccgacc gttggggcga gcagccaact ggtgttcgcg acgccggacg
     8041 taggcgacgc gacggcagcc ctgttcggca tcgaatcgcc gcgagagtac cacggccacg
     8101 accaaacccg cgccgaagtg accggccggc aggactggcg caacggcgtc gatctggtct
     8161 atctggggat cgcccgctat ctgcgcctgg gtgtcgatgg ccggccgccg caggtcgtcg
     8221 attgccggcc ggccgagacc gccgcgccca acttcgccac cctgaccgag atcgccgcgg
     8281 cgatcgacga cgccctcggc gccaacgtgg ccgacgtcgt cgagcgcgac ggcggccagg
     8341 tcctcgccct gcgctcgcca acgaccgggc agggcagccg cctggtggtc gaagcccact
     8401 cctccggcga cgcgcgggcg ctgctcttcg gcagcgtgcc cgacacgacg acgggcgtgg
     8461 atgcgcagcc cgcggtcatc accggcgagg tcgatctgct cgccccagcc gacctgagcc
     8521 gccgcagcgt actgcgattg gccatcgacg gcggcttgcc acaagacatc aacgtggccg
     8581 gctacgcgcc cggcgcgacg ctgctggaag aggtcgtcac ggccatcaac gccgccctgg
     8641 gcacggccga cggcccggcg gccgtcgccg cggccacggc cgagcagcgc ctgcaactga
     8701 cctcgccgac gaccggcccg gccagccggc tggaactcct gccgctgcgc accattgacc
     8761 tcatcgagta ccccgcggag acagccgcgc tgcccccgcg cacggtgcgg cacaatcaaa
     8821 gctggccggt ggacaatcgc ggcgcgctgg ccggcatggc cgaggtactg atcaccgccc
     8881 cgtcaggcgt ggccggcgtc gtgctggtca acgagacgct cggccaacag attcgcctgc
     8941 tgacggcgat tggcccgcgc caggcggcgc gcatctgggc cgacggcgcg cggctgcggg
     9001 ccgagattcg cccggcgagc ggcccggcgc ggcccgtgcc cgaccggcaa atcctcgccg
     9061 ggccgccggg ggcgcaggcg cgcgtgccct tccccggcga atggggcttc agcgggcgag
     9121 cgggcgagcg gccaacgctg caactcaaca accccaccgc cgacggcatc gtccgcctga
     9181 cggcccgcgc ggcgacggcg accggccgcc tggctctgcg cgtggccgac agcgacgtgc
     9241 ccccggcaac gctcgacgcc actgccgccg acggccagac cgttgccctg ccggggcgcg
     9301 tcgaggccac gggcgacggg ccagtctacc ggctgatggg caatggcggc gaggcgcggg
     9361 cggagttgcg cgccgggccg ggcgtggaga tagagcgctt cgttgatcgc gtcgtcgtcg
     9421 cccacggcac gatccacgcc gccgcgacgg gcgcgctgct gatcgtcagc cggctggagg
     9481 cgctcttccg ggcgacgctg acgctgacca cgccggcggg cgagccggtc gtcgaggagt
     9541 acgcccgcgt caccatcggc gacgacccgg ccgccgataa cgccctcgcg cgcgccatcc
     9601 agacgcggcc gtcggccctt gtgcgcgccg agtcgctggc caagatcgag gcgctgtcgc
     9661 tgccgctggg gcgttcgcgc tggcgcttcc tcgactgcta cgcgccgcgc ttcaactcgg
     9721 cccgcttcga ccgggcgcgc ttccccggcg aagtctgcca tgagcgcggc gtcttcgacg
     9781 ccagccgctt cgagaacgcg ccgccggagc cggtcgccgc cgccttcacc ggcatcgacg
     9841 acccgcccga cgccggggtg caggtgcagt tcacgaccgc gcgctaccag ccaggggcgt
     9901 tccgcgtcca ggtgccggcc gacctgccgg aggtcttcgg cggccggttc aaccaggccc
     9961 gctttggcca gggcaagaac cgcccggaac tatacccgtt cgttgttacc gagccgcccg
    10021 gcgacgagaa ccacgtcgag aaggtcttcg accactcccg gctggtggag gtggagatcg
    10081 tcccgcgcgt gccgctgggc tgggaaccgg tggccatgcc ctttcgcgag ccgcgcaccc
    10141 tgaccc
//
LOCUS       DAOSMQ010000052        10146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6619_length_10146_cov_4.715588, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000052 DAOSMQ010000000
VERSION     DAOSMQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6619_length_10146_cov_4.715588"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="PK829_02995"
     CDS             complement(<1..1343)
                     /locus_tag="PK829_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOU40214.1"
                     /translation="MTTRDRYPRRPPTPELPPMPESPDESRRPLGRVIGLALLLLLLA
                     LLLWGGLKAWRVAGAARSLLAAQEQAQALAAGGLAGLNPDAAEALLLQARADIVTLRD
                     ELAFSRPLAPHLGWVPRLGPTLVASPYLLDMADAGSEAAVLAVGSLKPALATVQGGDF
                     GMDNLGEVLPVLAAAGPALAQAGAALDRAAAARAGLAAAMPAEELPWRVRQLLALADE
                     WLPVGRDGLRLAPQLPSLLGADGPKRYLIMAQNEDELRATGGFVTGAGLVTVENGRIT
                     ALEFRDSSNIDNFAAQPYDFPPQPLYDYMNLELLAFRDANFWADFPTSAQKAMDLYAY
                     GLGVPPLDGAIAIDQEFLRLLVDATGPVPIPGSDRSINANNLLQVLRQARNIQEGQQV
                     GEWVADRKAFLGGFAAAIRARIESDFGSIDPVKLARNVVGAVETRHLSIYMRDPDV"
     gene            complement(1340..2746)
                     /locus_tag="PK829_03000"
     CDS             complement(1340..2746)
                     /locus_tag="PK829_03000"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU40215.1"
                     /translation="MTAATPRPRPALARPAGGPLLLAMLTAAIALAVYAPTVARDLTW
                     SGDSGELITAAMTLGVPHPPGYPTYVLLGRLFALLPVGLVAWRFNLFAAVCAAAAAGL
                     LAATIRRAGGARVRPVAAVAAALTFAFLPLVWSQAVVAEVYSLNLLMVATFLLVWAAR
                     GPGLAAGLCLGLAVTTHLTSLLLLPAALLAAGPGRARLLAGVAFGLLPLLALPWLARG
                     DSPVVWGQPDTLRGWWWLVSGQLYAANLRWPPDARLAGLLRALAVGPVALLAGRVVIG
                     RRVAALARPADRWPLLLGATAGLYALFALAYGTPDAAVLLLPALLLLAFLLAPRLNAP
                     GAWSVVLPLALAAAGLVARADSPLSPHAAATAALAAAPPRALLLTPGDRSLFALWYAH
                     HVEGQRPDVIIADTNLFAFDWYRLRLARQQPDLFVPATDDLLAFQRSNAAARPVCAVS
                     LTAAPASAPRLYCNEVTP"
     gene            complement(2743..4350)
                     /locus_tag="PK829_03005"
     CDS             complement(2743..4350)
                     /locus_tag="PK829_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU40216.1"
                     /translation="MTSDRLSVVVPAYNAAQTITACLTALGQQAGVGPYEVIVVDDGS
                     TDDTAALARAAGARVITTTRGRPAAARNTGARAAQGDILCFTDADCVPIADWLREISA
                     PFADPTVVATKGTYITHQTSLVARFVQLEYEEKYDRLRAQPVIDFVDTYSCAYRRAAL
                     LADGGFDERFHYLEDQELSFRLAARGLCMVFAERAVVAHHHSDTLARYLRKKATIGYW
                     KAQVVRRFPDRVVRDSHTPQIMKPQMALASLLPAALAVGLVALAAGGAAATWLLGGAG
                     LLALVFLATTLPFVARAARRDRAVALAAPALLFGRAAALSWGYARGVLFPRPGIGGTG
                     LSMFQAAVKRAVDVVGAMMGLLLTFLLAPFLFLLIRLDSAGPVFFRQERVGARGRSFV
                     MYKFRTMDVGAEEQLPALAAARGLSEPVLKLADDPRLTPAGHFLRRWSLDELPQFWNV
                     LRGEMSLVGPRLEELRVVAHYTEWQRRLSMRPGMTGPMQVSGRANLSLEERACIELDY
                     IDHYSLWRDAVILLRTIPVVLHGEGAQ"
     gene            complement(4347..5372)
                     /locus_tag="PK829_03010"
     CDS             complement(4347..5372)
                     /locus_tag="PK829_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU40217.1"
                     /translation="MITDKNTTTRPNWRRALPWLNLALSALLVLGGLWYLSTRVPLGD
                     VLAALAGASPWWVALSVGVFVVTLLLKGWRWQLLFPAGGAQVSFRTAFWGLMLGQYVN
                     LIIPFLRLGEVARLYALNRETGIAPARALGTLVVEKTLDLIFFGLTIVFILPFVILPD
                     YVNRPGPWLLGLPAALLLVLYVLAFRTTWVIGMWRRIIRPLPERLSGWLLRVAVSGLE
                     GLAALRDGRRSLLLLLASLLVAALSVLMPYTLFPALGLPLTLLDAALIHVVVSIAITP
                     PSTPAKVGVFNAAVALMLVQLGLTDETAVAGYAILFYLVVLVPQIALGLVAASRSHWH
                     WAAASQP"
     gene            complement(5421..5795)
                     /locus_tag="PK829_03015"
     CDS             complement(5421..5795)
                     /locus_tag="PK829_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40218.1"
                     /translation="MKRRWMAGTLAGLALLALVLALAGAVSAAPGNQDAYPAPSPTAD
                     PLTLPLDYPASVPAVVATPATIGAPVDGVPVVDVASPPNAATAAGDRGLVYLWIGFAA
                     TLLIFAASVIGAVLLFNRRTNP"
     gene            complement(5826..6107)
                     /locus_tag="PK829_03020"
     CDS             complement(5826..6107)
                     /locus_tag="PK829_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40219.1"
                     /translation="MINWLTIVAHASWIAGLALILAALSYHNWLAGQAGHSLRAELAG
                     QSFQRVLLVGMLLVGLGLSGISRGAWQQVLAGVVVVGAIAGLVWRWRRG"
     gene            6232..7605
                     /gene="radA"
                     /locus_tag="PK829_03025"
     CDS             6232..7605
                     /gene="radA"
                     /locus_tag="PK829_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU40220.1"
                     /translation="MAKRQTQYVCQACGRTTPVYMGRCPRCGEFDTMVEQRVDPEPGT
                     DRRRSAAAAVSRPVRLREVEADGLDRLSLPVAEFSRVLGGGLVPGSLILVGGDPGIGK
                     STLLLEVAGLVAGQHGPVLYISGEESARQIKMRADRLRIDSDDLFLVTETNLRVMMDH
                     INAVRPVMTIVDSIQTTYDDNLSSSAGSVSQVRESAARLQDVAKSTGVIVVLVGHVTK
                     EGAIAGPRVLEHIVDTVLYLEGDPFHRYRLLRSVKNRFGATSEVGVFEMVEQGMVEVS
                     NPSEAFLAERQANAPGSAIAVTLEGTRPLLVEVQALASSTTFANPRRTPNGIDTNRLL
                     LLTAVLTRRVHIPLADKDVFVNVIGGLQITEPAADLAVALAIASSVKDRAIHADMAFL
                     GEVGLSGELRAVSQLEPRLREAAKLGFRRCIVPRSRQLKEGSRPEGLEIIQCRTLHEA
                     VDVALIK"
     gene            complement(7652..9580)
                     /locus_tag="PK829_03030"
     CDS             complement(7652..9580)
                     /locus_tag="PK829_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,3-alpha-glucanase family
                     glycosylhydrolase"
                     /protein_id="HOU40221.1"
                     /translation="MLMPPRGPQRRPQRVRRRTAALPLLLLLLLLALGARATLPTAGA
                     QGQTKLVLAFYYAWYSPDSFGPGKTPFQPAAAYSSADGATIQRQVAEARSAGIDGFVQ
                     SWYGPQTANNQTETNFQTLLSVAAANGFTAAVDFETGSPFFASNDDRIAALNTLMSTH
                     ATQPAYLRVDGRPVIFFWASWLLSVSDWAAIREAVDPGHGAIWIAEGGDTTYLSVFDG
                     LHLYNTAWSAAPAQTAASWAGNTRAAAATYGSYKYWVATAMPGWDDTLLGRGTAAFRR
                     DRADGDYYRASFSGAAASSPDLLIITSFNEWAEGSQIEPSQEYGRFYLDLTAELSAAY
                     KSGSLAVAPPPPAAPSPGAATATAPTAATTAAATTTSPADTAIVADDAPAAATTAGAT
                     AGAIAAAPAATALADGRLLYTVVAGDTLLTIADRFGVRVADLRAYNGLAVDAVLMVGQ
                     SLIVGYTVFPDGARPLAGFPQARVRADGAVVHRVAAGDTPGGIALLYRLPLEELYSLN
                     GLEPGALLQIDQELIVGHEATPTAMPTATAMPTEALAATATVALPERAALATPAPSRT
                     PAPTPTATPTEPAAALAAVEDRGAPTLAAPPPPVTSGTAESQPSRRWLWLALGASVVV
                     MLGAAAVVARFSRIAARE"
     gene            9774..10067
                     /gene="rpsF"
                     /locus_tag="PK829_03035"
     CDS             9774..10067
                     /gene="rpsF"
                     /locus_tag="PK829_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOU40222.1"
                     /translation="MRDYEVTVVIQPQLEEAERNQVIERLSNLLVPGSKENGELVANH
                     WGVRQLAYPIRKFQEGYYVLFEAQLDPTRIRDIERSMQYNEDVLRYLVVRKEA"
BASE COUNT         1718 a         3373 c         3674 g         1381 t
ORIGIN      
        1 acgtcggggt cgcgcatgta gatggacagg tggcgcgtct cgaccgcgcc cacgacgttg
       61 cgcgccagct taaccgggtc gatggagccg aagtcggatt cgattctggc gcgaatggcg
      121 gcggcgaaac cgcccaggaa tgctttgcgg tcggccaccc actcgcctac ctgttgcccc
      181 tcctggatat tgcgcgcctg ccgcaggact tgcagcagat tgttggcgtt gatgctgcgg
      241 tcggagccgg ggatgggcac ggggccggtc gcatcgacca gcaggcgcag gaactcctgg
      301 tcgatggcga tcgcgccatc gagcgggggc acgcccaggc cataggcgta gaggtccatg
      361 gctttctggg ccgaggtggg gaagtcggcc cagaagttgg cgtcgcgaaa ggccagcaac
      421 tccaggttca tatagtcgta gagcggctgt ggcgggaagt catagggctg ggcggcgaaa
      481 ttgtcgatgt tgctggagtc gcggaactcc agcgcggtga tgcggccgtt ctcgaccgtg
      541 accaggcctg cgccggtcac aaagccgccg gtggcgcgca actcgtcttc gttctgggcc
      601 atgatcagat agcgcttcgg cccgtcggcg cccaacaagg acggcaactg aggggccagc
      661 cgcaggccgt cgcggccgac gggcagccac tcgtcggcca gggccagcag ttgccggacg
      721 cgccacggta actcctccgc gggcatggcc gcggccagcc cggcccgcgc cgccgcggcc
      781 cggtcgaggg ccgcgcccgc ctgcgccagc gccggcccgg ccgcggccag taccggcaac
      841 acttcgccga ggttgtccat gccaaagtcg cccccctgca ccgtggccag cgccggcttc
      901 aggctgccga cggccagaac ggccgcctcc gagccggcgt cggccatgtc gagcaggtag
      961 ggggaggcga ctaacgttgg tcccaggcgt ggcacccagc ccagatgggg cgcgaggggg
     1021 cggctgaagg ccagctcatc gcgcagggtg acgatgtcgg cgcgggcctg gagtagcagc
     1081 gcctcggccg cgtcggggtt gaggccggcc agcccgcccg cggccaacgc ctgggcctgc
     1141 tcttgcgcgg ccagcagtga gcgcgccgcg ccggccacac gccacgcttt cagcccgccc
     1201 cagaggagca gggccagcag caggagcaat agcgccaggc cgatgacgcg gccaagcgga
     1261 cggcgcgatt cgtcgggcga ctcgggcatt ggcgggagtt cgggcgttgg cggacgccgt
     1321 ggatagcgat cgcgggtagt caaggcgtta cctcattaca gtacaaacgt ggggcagagg
     1381 ccggcgcggc cgtcaggctg accgcgcaga ccggccgcgc ggccgcgttc gaccgctgga
     1441 aggcgagcag gtcgtcggtg gcgggcacga aaagatcggg ctgttgccgc gccagccgca
     1501 ggcgatacca gtcgaaggcg aagagattgg tgtcggcgat gattacatcc ggccgctgcc
     1561 cttcgacgtg gtgggcatac cacagggcga agaggctgcg gtcgcccggc gtcagcagca
     1621 gcgcgcgcgg cggcgcggct gccagggcgg ccgtggccgc ggcgtggggc gagagcgggc
     1681 tgtcagcgcg ggccaccagc ccggccgcgg ccaaagccag cggcagcacg accgaccacg
     1741 cccccggcgc gttgagccgc ggggccagca ggaaggccag cagcaacagc gccggcagca
     1801 gcagcacggc ggcgtcgggc gtgccgtagg ccagggcgaa cagggcgtag aggcccgctg
     1861 tcgcgcccag caggagcggc caacgatcgg cggggcgggc cagggcggcc acgcggcggc
     1921 cgatgaccac tcgcccggcc agcagcgcca ccgggccgac ggccagcgcc cgcagcaggc
     1981 cggccagccg cgcgtcgggc ggccagcgca gattggcggc gtagagctgg ccgctgacca
     2041 gccaccacca gccccgcagc gtgtccggct gcccccagac cacggggcta tcgccacggg
     2101 ccagccaggg cagggccagc agcggcagca gaccgaaggc gaccccggcc agcagccgcg
     2161 cccggcctgg cccggccgcc agcagcgccg ccggcaagag caagagcgag gtcagatgcg
     2221 tagttacggc cagccccagg cagaggccgg cggccaggcc ggggccgcgc gcggcccaga
     2281 ccagcaggaa ggtagccacc atcagcaggt tcaggctgta gacctcggcc accacggcct
     2341 gactccacac cagcggcagg aaggcgaacg tcagcgccgc ggcgacggcg gcgaccggcc
     2401 ggacgcgcgc gccacctgcg cgccggatgg tcgccgccag cagaccagca gcggcggcgg
     2461 cgcagacggc cgcgaacagg ttgaagcgcc aggccaccag gccaaccggc aacagggcaa
     2521 agagccgccc caataggacg taggtggggt aaccgggcgg gtggggcacg cccagggtca
     2581 tggccgcggt gatgagttcg ccgctgtcac cggaccaggt caggtcgcgg gccacggtgg
     2641 gcgcgtagac ggccagggcg atggcggcgg tgagcattgc cagcagcagg gggccaccgg
     2701 cagggcgagc gagggccggc cgtggccggg gcgtggcggc ggtcattgcg ctccctcgcc
     2761 gtgcaacacg acggggatgg ttcgcagcag gataacggcg tcgcgccaca gactgtagtg
     2821 gtcaatgtag tccagttcga tgcaggcgcg ctcctcgagc gacagattgg cccggccgct
     2881 gacctgcatc ggcccggtca tgcccggtcg catcgacagc cgccgctgcc actcggtgta
     2941 gtgggccaca acgcgcagtt cctccagccg cggcccgacc aggctcatct cgccgcgcag
     3001 gacgttccag aactgcggca gttcgtccag gctccagcgg cgcagaaagt ggccggcggg
     3061 tgtcagccgc gggtcgtcgg ccagcttcag cacaggctcc gacaaccccc gcgccgccgc
     3121 cagcgccggc agttgctcct ccgcgccgac gtccatcgtc cggaacttgt acatgacgaa
     3181 gctccgcccg cgtgcgccga cccgctcctg ccggaagaag accggcccgg ctgaatccag
     3241 gcggatgagc aggaacagaa agggggccag caagaacgtg agcaacagtc ccatcatggc
     3301 cccgaccacg tctacagcgc gcttgacggc ggcctggaac atgctcaggc ccgtgccgcc
     3361 gatgcccggc cgcgggaaca ggacgccacg ggcatagccc cagctcaggg ccgcggctcg
     3421 cccaaatagc agcgccggcg cggccagggc cacagcgcga tcacgccgcg ccgcgcgggc
     3481 cacaaagggc agcgtcgtcg ccaggaagac cagcgccagc agcccggccc cgcccagcag
     3541 ccacgtcgcg gccgccccgc cggccgccag cgcgaccagc ccgaccgcca gcgccgccgg
     3601 caatagcgac gccagagcca tctgcggctt cataatctgc ggcgtgtggg agtcgcggac
     3661 gacgcggtcg ggaaaacgcc gcacgacctg cgccttccag tagccaatgg tcgctttctt
     3721 gcgcaggtag cgagccagtg tgtcgctatg atggtgggcg acgacggctc gctcggcgaa
     3781 gaccatgcac aggccgcggg cggccagccg gaaggatagt tcctgatctt cgaggtagtg
     3841 gaagcgctcg tcgaagccgc cgtcggccag cagcgcggca cgccggtagg cgcaggagta
     3901 ggtatcgacg aaatcaatga ccggctgggc gcgcaggcgg tcgtactttt cctcgtattc
     3961 gagctgcacg aagcgggcga cgagggacgt ctggtgggtg atgtaagtgc ctttggtggc
     4021 aacgacggtg ggatcagcga atggcgcgct aatctcgcgg agccaatcgg cgatcggcac
     4081 gcagtcggca tcggtgaagc agaggatatc gccctgggcc gcccgcgcgc cggtgttgcg
     4141 ggccgccgcc ggccggccgc gcgttgtggt gatgacgcgc gcgccggcgg cgcgggccag
     4201 ggcggcggtg tcgtcggtcg agccatcatc gacgacgatg acctcgtatg gccccacgcc
     4261 ggcctgctgc cccagggcgg tgaggcaggc ggtgatggtc tgggcggcat tgtaggccgg
     4321 gacgacgacg gagagccggt cgctggtcat ggctgggagg cggccgccca gtgccaatgg
     4381 ctgcgcgagg cggcgacgag accgagggcg atctggggca cgaggacgac caggtagaac
     4441 aggatggcgt agccggctac ggctgtctcg tcagtcagac ccaactgcac caacatcagg
     4501 gccacagcgg cgttaaacac gcccaccttg gccggcgtgc tgggcggggt gatggcaatg
     4561 gagacgacca catggatgag ggccgcgtcg agcagcgtca ggggcagacc gagggcgggg
     4621 aagagcgtat agggcatcag gacggagagc gcggcgacga gtagcgaggc cagcagcagc
     4681 agcagcgagc gccggccgtc gcgtagcgcc gccaacccct ccagccccga cacggccacg
     4741 cgcagcagcc agccggacag ccgttcgggc agcggccgta tgatccgccg ccacatgcca
     4801 attacccagg tcgtccgaaa ggcgagcacg tagagcacga gcaacagcgc cgccggcagg
     4861 cccagcagcc aggggccagg tcgattgaca taatccggca gaatgacaaa cggcaggatg
     4921 aagacgatgg tgagtccgaa gaaaatgagg tcgagcgtct tctcgaccac cagcgtcccc
     4981 aacgcccgag ccggggcgat gccggtctcg cggttcaggg cgtagagccg cgccacttcg
     5041 ccgaggcgca ggaaggggat gatgaggttg acgtactgcc ccagcatgag gccccaaaag
     5101 gcagtccgga aggacacctg cgcgccgccg gccggaaata gcagttgcca gcgccaaccc
     5161 ttcagcagga gcgtgacgac gaagacgccg accgacagcg ccacccacca cggcgacgcc
     5221 ccggccagcg cggccagcac atcgcccagg gggacgcgcg tggacaggta ccacaggcca
     5281 cccagcacca gaagggcgct cagggccaga ttcagccagg gcagcgcccg tcgccagttg
     5341 ggcctggtcg tggtgttctt gtcggtgatc aaggctgcct gctctttgtt ggcggtgagg
     5401 gtgatgggcg cggcgcggct ctaagggttg gtgcgccggt tgaatagcaa gaccgcgccg
     5461 atgacgctgg cggcgaagat gagcagggtg gcggcgaagc cgatccacag gtagacgagt
     5521 ccacgatccc cggccgcggt tgccgcgttg ggcggcgaag cgacatcgac cacggggacg
     5581 ccatcgaccg gcgcgccgat cgtcgccggc gtggccacca cggcggggac ggatgccggg
     5641 tagtcgagcg gcagcgtcag cgggtcggcc gtggggctgg gggcggggta agcgtcctgg
     5701 ttgccgggcg cggcgctgac cgcgccggcc agcgccagca cgagcgccag caaggccagg
     5761 ccggccagcg tgccggccat ccaacgtcgc ttcatatcac ttttctccgt ccatttggcc
     5821 acgtgtcagc ccctgcgcca gcgccacacc agaccagcga ttgccccgac gacgaccaca
     5881 ccggccagca cctgctgcca cgcgccccgg ctgatgccgc tcaggccgag gccgaccagc
     5941 agcatgccga ccaggaggac gcgctggaac gactgcccgg ccagctccgc gcgcagcgag
     6001 tggccagcct gtccggccag ccagttatgg tagctcaacg ccgccaggat cagcgccagc
     6061 ccggcaatcc aggaggcgtg ggcgacaatc gtcagccagt tgatcatgtc agttatctag
     6121 tatatcagcc ccgccgcggg cagcccgcac agccggcgtg aattcccacc ggcggctaag
     6181 cgatcgggtg catgtctccg ccccggtccc gaacacgggt atacttggga tatggccaaa
     6241 agacaaaccc aatatgtatg ccaggcgtgc ggccgcacca cccccgtcta catggggcgc
     6301 tgcccgcgtt gtggagaatt cgatacgatg gtcgaacagc gcgtcgatcc ggagccgggc
     6361 actgaccggc ggcgctcggc ggcggcggcc gtctcgcgcc ccgtccgcct gcgcgaggtc
     6421 gaagccgacg gtctcgaccg gctgagcctg cccgtggcgg agttttcgcg tgtgctgggc
     6481 ggcggccttg tccccggctc gctgatcctg gttgggggcg atcccggcat cggcaaatcg
     6541 accctgctgc tggaagtggc cggcctggtg gccgggcagc acgggccggt gctctacatc
     6601 tctggggagg agagcgcccg ccagatcaag atgcgcgccg accgcctgcg catcgactcc
     6661 gatgatctct ttctggtgac cgagaccaat ctacgggtga tgatggatca catcaacgcc
     6721 gtgcggccgg tcatgaccat cgtcgatagc atccagacga cctatgacga caatctcagc
     6781 tcctcggctg gcagcgtcag ccaggtgcgc gaatcggctg cccggctgca agacgtggcc
     6841 aagagcacgg gcgtcatcgt cgtcctcgtc ggccacgtca ccaaagaggg cgctatcgcc
     6901 gggccgcgcg tgctggagca catcgtcgat accgtcctct atctggaggg cgatcctttc
     6961 caccgctatc gccttctgcg cagcgtcaag aaccgcttcg gggcgacgag cgaggtgggc
     7021 gtctttgaga tggtcgagca gggcatggtc gaagtgtcca atccgtcgga ggcgtttttg
     7081 gccgaacggc aggccaacgc gcccggctcg gccattgccg tgacgctgga aggcacgcgg
     7141 ccgctgctgg tcgaggtgca ggcgctggcc agttcgacta cctttgccaa cccgcgccgc
     7201 acccccaacg gcattgatac caaccggcta ctgctgctaa cggccgtgct gacccgccgc
     7261 gtccacatcc cgctggccga caaggacgtt tttgtcaacg tcattggcgg cttgcagatc
     7321 accgagccgg cggccgacct ggccgtggcc ctggccattg ccagcagtgt caaggaccgc
     7381 gccatccacg ccgatatggc tttcctgggg gaggttgggc taagcggcga actgcgtgcc
     7441 gtcagccagc tagagccgcg cctgcgcgag gcggccaaac tcggcttccg gcgctgtatc
     7501 gtgccccgca gccgccagct taaagagggc agccggccgg aggggctgga gatcatccag
     7561 tgccggacgc tgcacgaggc ggtcgatgtc gcgttgatca agtaagaggg tggcgcgatt
     7621 ctcccgaatc gcgctattcg cgtaaatcgc gctattcgcg tgccgcgatt cgggagaatc
     7681 gcgccacgac ggccgccgcg cccagcatca ccacgacact cgcgcccaac gccagccaga
     7741 gccaacgccg cgacggctgg ctctcggccg tgccggacgt gactggtggg ggaggggccg
     7801 ccagcgtggg cgcgcctctg tcctcgaccg ctgccaatgc cgcggccggc tctgtcggcg
     7861 tggcggtggg ggtcggggcc ggcgtgcggc tgggcgcggg cgtcgccagg gccgcacgct
     7921 ccggcagggc gaccgtggcg gtggcagcca gtgcctccgt gggcatggct gttgccgtgg
     7981 gcatggccgt cggcgtggcc tcatggccga caatcagctc ctggtcgatc tgcaacagcg
     8041 cgcccggttc cagcccattg aggctatata actcctctaa cggcagccgg tagagcaggg
     8101 cgatgccgcc cggggtgtcg cccgcggcca cgcggtggac gaccgcgccg tccgcgcgga
     8161 cacgcgcctg cgggaacccg gccagcggac gcgcgccgtc gggaaagacc gtgtagccga
     8221 cgatgagcga ctggccgacc atcagcacgg catcgaccgc caggccgttg taggcccgca
     8281 ggtcggccac gcgcacgcca aagcggtcgg cgatggtcag cagcgtgtcg ccggcgacga
     8341 cggtgtagag aaggcggccg tcggccagtg ccgtggccgc cggggcggcc gcgatggcgc
     8401 cggcggtagc gccggccgtg gtggctgccg ccggtgcgtc gtcggccacg atggctgtgt
     8461 cggccggcga ggttgtggtc gcggccgcgg ttgtggccgc ggtcggggcc gtggctgtgg
     8521 ccgcgcccgg cgagggcgcg gccggaggag gtggagctac ggccagcgat ccactcttat
     8581 aggcagcgct tagctcggcg gtcaggtcga ggtagaagcg accatactcc tgcgacggct
     8641 cgatctggct gccctcggcc cactcgttga acgaggtgat gatgagcagg tcggggctgc
     8701 tggccgccgc gccgctgaag ctggcccggt agtagtcgcc gtcggcgcgg tcgcggcgga
     8761 aggcggccgt gccccggccc agcagcgtgt cgtcccagcc gggcatggcc gtggccaccc
     8821 agtacttgta gctgccatag gtggccgctg ccgcccgcgt gttgcccgcc caggaggcgg
     8881 ctgtctgggc tggcgcggcc gaccaggctg tgttgtagag gtgtagaccg tcgaagacgc
     8941 tcagataggt cgtgtcgcca ccctcggcga tccagatcgc cccgtggccg gggtcaacgg
     9001 cctcgcgaat ggccgcccag tccgacacgc tcagcagcca gctggcccag aagaaaatga
     9061 ccggccggcc gtcgacgcgc aagtaagctg gctgtgtggc gtgggtgctc atcagcgtgt
     9121 tcagcgcggc gatgcggtca tcgttgctgg cgaagaaggg gctgccggtt tcgaaatcga
     9181 ccgcggcggt gaagccgttg gccgcggcga cactcaagag cgtctggaag ttggtctccg
     9241 tctggttgtt ggccgtttgt gggccatacc agctttgcac gaagccgtcg atgccggcgc
     9301 tgcgggcctc ggcaacctgg cgctggatgg ttgcgccgtc ggccgagctg taggccgctg
     9361 ccggctgaaa gggcgttttg cccggcccga aggagtcagg gctataccaa gcgtagtaaa
     9421 aggccaacac gagctttgtc tgcccttgcg ccccagccgt gggcagggtc gcccgcgcgc
     9481 caagcgccag caggaggagc agcagcagca agggcagggc ggccgtgcgg cggcgaacac
     9541 gctgtggcct acgttgtggc ccacgcggtg gcataagcat cggcgcgttc atatcaccgg
     9601 cgcgccaatc cggcaaacgc gccgccgatc ggccttgcct ttttctaagg agacgctata
     9661 atcgcgaatt cgttgccggc aatggccgca cgccattccg ccgcttccgc tcccggcccg
     9721 tgtctggggg caatgctcgc aagagtaaat tccaaggaaa ggagaatcgc aacgtgcgtg
     9781 actacgaagt gacggttgtt atccaaccgc agttggaaga agctgagcgc aatcaagtga
     9841 tcgagcggct cagcaatcta ctggttcccg gctccaaaga gaacggcgaa ttggtcgcca
     9901 atcattgggg cgtccggcag ctggcctacc ccatccgcaa gttccaggaa ggctactacg
     9961 tcctcttcga ggcgcagcta gacccgacgc gcatccgcga catcgagcgc agcatgcagt
    10021 ataacgaaga tgtccttcgt tatctggtcg tgcgcaaaga agcctaagtc ctgcaggcag
    10081 gacatttcgc gatggcgtcg aacagaggct tgaacaaagt catggtcatc ggctggctgg
    10141 aggacg
//
LOCUS       DAOSMQ010000053        10120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6644_length_10120_cov_4.387183, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000053 DAOSMQ010000000
VERSION     DAOSMQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6644_length_10120_cov_4.387183"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..1540
                     /locus_tag="PK829_03040"
     CDS             389..1540
                     /locus_tag="PK829_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU40223.1"
                     /translation="MIDSEFGNGEHPMSFLLTGDVYLPSAGEIRLGRIVALRSNEILV
                     DIGAKSEGVIPSQETSTYDTTTRAQLQEGEDILVFVLNPEDQNGNIILSFRRAAEERD
                     WQVATDLFESQEALETEIVGFNKGGVLVSVGQLRGFVPNSQLRRDRLQYIDSDNKQRS
                     YQALVGQPMLAKVIEVDRPRNRLILSEQAASREIRQARKTELFSSLQEGAVCEGVVVN
                     LADFGAFVDIGGIEGLVHLSEMSWKRTQKPADILQKGDHVKVQIMSVDQERQRVALSM
                     KRLEADPWTRIEELYRVGQLVEAEITRLTKFGAFARVDDDYQLEGLIHISELADDRVE
                     QARDIVKPGDRVTVRVIRLDAKQRQVGLSLRQVASDRYIEEDLAMMNPM"
     gene            1636..4188
                     /locus_tag="PK829_03045"
     CDS             1636..4188
                     /locus_tag="PK829_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOU40224.1"
                     /translation="MNSKNWERFTQRARRVLSLAQEEAERLNHSYIGSEHVLIGLLLE
                     EGGVAGRVLRELGLEVTRVQAMVERLSGGQGTRTPFTKTELSPSTKRILEYAVEEARR
                     MGQHYISTEHLLLGLARQNEGLAIDVLRKFGISAEQIRRQTRRMLKESPVSGSESGSQ
                     QQQPQRRPAQQQQKKEKSKTPLVDQLATDLTALAQEYALDPVIGRETEIERVIQILAR
                     RTKNNPALIGQPGVGKTAIVEGLAQRIVDGQVPESLHNKRVLQLDVGSLVAGTMYRGQ
                     FEERLKRVIEELKSSDAILFIDEVHMLVGAGSAGSSVDAANILKPSLARGELQCIGAT
                     TLDEYRKYIESDAALERRFQQVRVDEPSAEETVQILKGIKGAYEQHHNLLISDEAIEA
                     AVFLSTRYVTERYLPDKAIDLIDEGASRVRMYRVQQPTDIREAYDSLRELRVQRNEAV
                     EAGRYDDATRIREQEEEIQHQLDDLRAGQVEYDQSTMLTAEDVAEVLSMWTGIPVYQL
                     TEEESARLLRMEEELQKAIVGQKDAINAIAKAVRRARAGLKDPRRPIGSFMFLGPTGV
                     GKTELTKALATFLFGSEDALVQLDMSEFMERHNVARLVGSPPGYVGYEDAGQLTEAVR
                     RRPYSIVVFDEIEKAHPEAFNLLLQIMEEGHLSDAKGQKVNFRNAIIILTSNVGADMI
                     KKGGGLGFNFKQDAAVLVEENYRDMQKKLMEQLKRHFRPEFLNRLDSVIVFRQLQPED
                     IRQIVDIQIADVNERMDERFLLLEATDAAKDWLARHGYDAEYGARPLRRLIQQTVEDK
                     LSDAVLAGEFKEGETVIIDIGPGASAEGEIVLRHATLEEAGEQPALPAIQAN"
     gene            4469..6508
                     /locus_tag="PK829_03050"
     CDS             4469..6508
                     /locus_tag="PK829_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOU40225.1"
                     /translation="MLSTFERAMFLLLAAVCLTAVYNTFRQMALVINRGQGALALNDL
                     PRRVMAGLLALLTQGRILRHRKITSLFHYGVAFGFIFYGLVNLIDILEGVVPGFSFLH
                     DNIIGQLFRLAADLFAAAVLIGVVYFLLRRFAAHDPALKTRGNVKLMERVREGGIAVD
                     SLIVGLFILAHVGSRLMGAAFKVALDGPDGWQPVASFIAATFLRGLSPDALTLGFHAF
                     WWLAIGLIFLFLPYFPYTKHAHLFMGPTNFMLSPDRTYLGQMKKLNLDDETIEQFGAN
                     DLDDLPKKHILDAFACIMCNRCQQVCPAYNTGKELSPAALEINKRYYIRQNMPELAAG
                     AANGTPLLDYAISPSAVWACTACGHCIEICPVGNVPMLDIMEIRRDQVMMQSEFPEQL
                     KGAFVGMERMGNPWQAQASRMEWTAPLPFDVPTVAENPDYEYLLWVGCAGAFNPDAQK
                     TVQALATILHTAGVSFAILGDDETCTGDSARRAGNEPLFQALAEANIETLNAARANER
                     RIVTSCPHCFTTLGKEYGELGGHYTVFHHTQLIADLVGKGRLRLDGGTLTQATFHDPC
                     FLGRHNGVLDEPRAALAAAGMTLLEMPRHGKDSFCCGAGGAQYWKEEEHGTEAVNVNR
                     YREARATGAAVLAVGCPFCNTMMSDANREQGAPMAVKDVAEVVVEALRGKVGVAN"
     gene            6614..>10120
                     /locus_tag="PK829_03055"
     CDS             6614..>10120
                     /locus_tag="PK829_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU40226.1"
                     /translation="MVTVLPVDAALLTIYPRQPLSVSVALPAETLSLPTPASETRTSE
                     TQASETQASLLINEIDAIAASEAAEFIELYDGGVGNTPLDGLTLVLYRGDRASVYLAI
                     GLDGARTDSRGYYVLGGPAIDADQLLAAGLLRDGPDAVALFAASVWDFPVNSPVVVEG
                     LLDAVVYAPRGTPATGLQPLLLPDELPVDEGQRGAAEAHSAQRFPNGQGQAREMAAFT
                     AGPPTPDEPNKKSLDYAPTVTNVWPMPDASDVPNTLTISVTFSEPVMLHDTPITLACE
                     RSGVHPYALNGGPLTYGFRPYIALASGDACAVTVYGDRVSDMDEDDPPDGMLASYKWY
                     FTAAQTVAQNIVLNEIDADTPSTDTAEFIELFDGGGGATPLDGLLLVLFNGGDDRSYR
                     TIDLAGYTTDAAGYFVLGNAAIVGAEIELPDGALQNGTDAIALVAGRAEAFPNGTPVT
                     GVTPLDAVVYGRSDEPDAGLLPLLNDGQPAIDENSRGEAESHSNQRCPNGAGGARNTA
                     GFRQNTPTPRAASDCQTDVAPGVSERSPAPGATGVSVSVTLRIDFTEPVSVGKSWLTL
                     ACSTSGAHSSTTTGGPSAFFIQPNEPLAYAETCTVTLKAALIHDLDTDDPPDTLTGNL
                     TWKFTTAEAPAAFVIINETDADTPGSDTSEFIELYDGGRGATRLDGLVLVFYNGATNT
                     AYTALDLDGQRTDSAGYWLAGNAAVTPGLAFANGTLQNGPDAVALYAGDATQFPADTP
                     LHTRGLLDAVVYGEATNTPGLLALLLPGESAADEGARGAADAHALQRCPNGAGGQRRT
                     AALRPAPPTPGKTNICALDAAPVVTAVVPSNGETGVAIDVALTVTFSEPVAPVAGWLR
                     LRCGNNDYAINVTAADPARYTATPTTLLPRAATCYATIAAALVHDADNDDPPDILAAD
                     YTWSFATAAPVADFVLINELDSDTPGSDTAEFVELYDGGVGGTDLSGLVLVFFNGTDD
                     HAYFAVDLDGVQTEGDGYAVAGSAALGGALTLPAAMIQNGPDAVALYEGDAADFPNGT
                     GITTDGLRDAIVYGTSDPTDNGLLALLAAGQAQVDENARAAAEADSSGRCPNGAGGRR
                     YTATYRQNAPTPGRANDCHADAPPAVAAVSPTDGADHVAAAAQLQVEFSEKVTLDGNW
                     FGIVCDASGSHTATTGGGPTTFT"
BASE COUNT         1923 a         3464 c         3110 g         1623 t
ORIGIN      
        1 agggccagct ggaggctcat gctcagcgcg cccagccatg tcgtcgtcac gccggggtgg
       61 ccggccacca gcgtgcccgc ccagtggcca gcgagcagcg cctggcggaa ctgcatcgag
      121 cggtagaccc acaccagttc gtcgggggtg atgtagcggc ccacggccac cagccgcggg
      181 acgagactga cgaaaaagag gagaacgggg aataaccgca gccaagggcg tgtcgccgac
      241 ggccctgtcg cagagagaag agaagcacgc ccggccattc ccctacgtat acagcacatt
      301 agacatattg ccaagcggtc taaacgcagt ataatcgctc gaaaatgggc gctggggcaa
      361 cctcggcgca ggtgggaatc ttagcaacat gatcgactcc gaattcggca acggcgaaca
      421 tccaatgagc tttctgctca caggcgatgt ctacctaccc agcgccggcg aaatccgcct
      481 cggccgcatt gtagccttac gaagcaacga gatactggtc gatattgggg caaagtccga
      541 gggagtcatc ccttcgcaag agacctcgac atacgacacg acgacgcgtg cccaattgca
      601 ggaaggcgag gacatcctcg tcttcgtgct gaaccccgaa gatcagaacg gcaacatcat
      661 cctgtcgttc cgccgcgcgg ccgaggagcg cgattggcag gtggccaccg acctctttga
      721 gtcgcaagaa gcattggaga ccgagatcgt cggcttcaac aagggcggcg tgttggtcag
      781 cgtgggccag ctgcgcggtt tcgtgcccaa ctcccagttg cggcgcgacc gcttgcagta
      841 catcgacagc gataacaagc agcgctccta ccaggccctc gtcggccagc ccatgttggc
      901 caaagtgatc gaggtcgacc ggccacgcaa ccgcctgatc ctgtccgaac aggcggcctc
      961 gcgggagatc cgccaggcgc gcaagaccga gttgttctcg tcgctgcaag agggcgcagt
     1021 ctgtgagggc gtggtcgtca atctggccga tttcggcgcg ttcgtcgaca tcggcggcat
     1081 cgagggtctt gtccacctct cagagatgag ttggaagcgt acccagaagc cggccgatat
     1141 cttgcagaag ggcgaccatg tgaaggtgca gatcatgagc gtcgatcagg agcggcagcg
     1201 cgtcgccctc agcatgaaac ggctggaggc cgacccgtgg acgcggatcg aggagctata
     1261 ccgtgtcggc cagctggtgg aggcagagat tacccgcctg accaagttcg gagcttttgc
     1321 ccgcgtggac gacgattatc aattggaggg gttgattcat atttcggagt tggccgatga
     1381 ccgcgtcgag caggcgcgcg atatcgtgaa gccgggcgac cgggttacgg tgcgcgtcat
     1441 tcgtctcgat gccaaacaac gccaggtcgg cctgagcctg cgtcaggtcg cctcagacag
     1501 atacatcgaa gaagacctgg cgatgatgaa tcccatgtaa atggcaacgt gccccctggg
     1561 cctggcccac ggcgcacgcg atgatccgat ggcgactacc cgtgaacgta ttgagtttcc
     1621 ctgaggtgga tagccgtgaa ttctaagaat tgggaacgct ttactcaacg cgcgcgacgc
     1681 gtgttgagct tggcccagga agaggccgag cgcctcaacc atagctacat aggcagcgag
     1741 cacgtcctga ttggcttgct gctcgaggag ggcggcgtgg ccggccgagt tctgcgcgaa
     1801 ctgggcctgg aggtcacgcg ggttcaggcc atggtcgaac gcctatcggg cggccaggga
     1861 acccggacgc cattcaccaa gaccgaactt tcgcccagca cgaagcgcat cctggaatac
     1921 gccgtcgaag aagcgcgacg catgggccag cactacatca gcacagagca tctgctcctc
     1981 ggcctggccc gccagaacga gggcctggct atcgatgtcc tgcgcaagtt cggcatcagc
     2041 gccgagcaga tccgccggca gacgcggcgc atgctcaagg aaagccctgt ctccggcagc
     2101 gagtcgggtt cgcagcagca acagccgcaa cgtcggccgg cgcagcagca gcagaagaaa
     2161 gagaagagca aaacgcccct ggtcgaccag ctggccaccg acctgaccgc cctggcgcag
     2221 gagtatgccc tggacccggt catcggccgc gagacggaga tcgagcgcgt catccagatt
     2281 ctggcccggc gcacgaagaa caaccccgcc ctcatcggcc agcccggcgt cggcaagacg
     2341 gccatcgtcg agggcctggc ccaacgcatc gtcgatggcc aggtgccgga atcgctgcac
     2401 aataagcgcg tcttgcaact ggacgtgggc agcctggtcg ccggaacgat gtaccgcggc
     2461 cagttcgagg agcgtctcaa gcgcgtcatc gaggaactca agtccagcga cgcgatcctg
     2521 ttcatcgacg aagtccacat gctcgtcggc gcggggtcgg ccggcagctc ggtcgacgcg
     2581 gccaacatcc tcaagccgtc gctggcccgc ggcgagttgc agtgcatcgg ggcgacgacg
     2641 ctggacgagt accgcaagta catcgagagc gatgctgccc tggaacgccg cttccagcag
     2701 gtgcgcgtcg atgagccgtc ggccgaggag actgtccaga ttctcaaggg catcaaggga
     2761 gcctatgagc agcatcacaa tctgctcatc tccgacgaag ccattgaggc ggctgtcttc
     2821 ctgtccacgc gctacgtcac cgagcgctac ctgcccgaca aggccatcga cctgatcgac
     2881 gagggcgcgt cgcgcgtgcg catgtaccgc gtccagcagc cgaccgacat ccgcgaggcg
     2941 tatgactcgc tgcgcgagct gcgcgtccaa cgcaacgagg ccgtcgaggc cgggcgctac
     3001 gacgacgcta cccgcatccg cgaacaggaa gaggaaatcc agcatcagct cgatgacctc
     3061 cgcgccgggc aggtggagta tgaccaatcg acgatgctga cggccgaaga cgtggccgag
     3121 gtgctgtcga tgtggaccgg cataccggtt taccagctca ccgaagaaga gagcgcccgc
     3181 ctgctgcgca tggaagagga gttgcaaaag gccatcgtcg gccagaagga tgccatcaat
     3241 gccatcgcta aggccgtccg gcgcgcgcgc gcgggcctga aggacccccg gcggcccatc
     3301 ggctcgttca tgttcctcgg ccccacgggc gtgggcaaga ccgagctgac caaagccctg
     3361 gccaccttcc tcttcggcag cgaggacgct ctggtgcagc tcgatatgtc cgagttcatg
     3421 gaacgacaca acgtcgcccg gctcgtcggg tcgccgcccg gctacgtcgg ctatgaagat
     3481 gccggccagc tgaccgaagc cgtccgccgc cggccctact ccatcgtcgt cttcgacgag
     3541 atcgagaagg cccaccccga agccttcaac ctgctcttgc agatcatgga agagggccac
     3601 ctgtccgatg ccaagggcca gaaggtcaac ttccgcaacg cgatcatcat cctgacctcc
     3661 aacgtcggcg cggacatgat caaaaagggc ggcggcctgg gcttcaactt caagcaagac
     3721 gcggccgtgc tcgtcgaaga aaactaccgc gacatgcaga agaagctcat ggagcagctc
     3781 aaacgtcact tccggccgga gttcctcaac cgcctcgata gcgtcatcgt cttccgccag
     3841 cttcagccgg aggacatccg ccagatcgtc gatatccaga tcgccgacgt gaacgagcgc
     3901 atggacgagc gcttcctgct gctagaggcg accgacgcag ccaaagactg gctggcccgg
     3961 cacggctacg atgccgagta cggcgcgcgg ccgctgcggc gtctcatcca gcagaccgtt
     4021 gaggacaagc tctccgatgc cgtcctggcc ggcgagttca aggaaggcga gacggtgatc
     4081 atcgacattg gcccgggggc gagcgccgag ggcgagatcg tcctccgcca cgccacgctg
     4141 gaagaagccg gcgaacagcc cgccctgccc gccattcagg ccaattagac cagcgcgcca
     4201 accaggcgag atcaaccggg tttctgggtg aaacccggtt ttttttgggc ttatgacctg
     4261 acaggtgagc cgcctacctg tcaggtcttt ggcaaacccc cgcgcggccc ggtagacaaa
     4321 taatgcagtg cgttagatat gcgttatact aggggcgtac caggcggcgc tagtccgacc
     4381 aggcgctgcc taaaccgtta acccgcgccg accgtcgctc ccccttcctc aatcatccgc
     4441 ggcgacgatc gcaaggagag gaacaaccat gctatcaacc ttcgaacgag ccatgttcct
     4501 gctgctggcc gccgtctgcc tgaccgccgt ctacaatacc ttccggcaaa tggccctcgt
     4561 catcaaccgc ggccagggcg cgctggccct caatgacctg ccgcgccgcg tcatggccgg
     4621 cctgctcgcc ctgctgaccc agggccgcat cctccgccat cgcaaaatca cctccctctt
     4681 ccactatggc gtcgccttcg gcttcatctt ctatggtctg gtcaacctca tcgacatcct
     4741 cgaaggcgtc gtccccggct tcagcttcct ccacgacaac atcatcggcc agctcttccg
     4801 cctggccgcc gacctcttcg ccgccgccgt cctcatcggc gtcgtctact tcctcctgcg
     4861 acgcttcgcc gcccacgacc cggccctcaa gacccgcggc aacgtcaagc tcatggagcg
     4921 cgtccgcgag ggcggcatcg ccgtcgattc gctcatcgtc ggcctgttca tcctcgccca
     4981 cgtcggctcg cggctcatgg gcgcggcctt caaagtcgcc ctcgatggcc ccgacggctg
     5041 gcagcccgtg gccagcttca tcgccgccac cttcctgcgc ggcctctcgc ccgatgccct
     5101 taccctcggc ttccacgcct tctggtggct ggccatcggc ctcatcttcc tcttcctgcc
     5161 ctacttcccc tataccaaac atgcccacct cttcatgggg ccgaccaact tcatgctcag
     5221 ccccgaccgt acctacctgg gccagatgaa gaagctcaac ctcgacgatg agaccatcga
     5281 acagttcggg gccaacgacc tcgacgacct gcccaaaaag cacatcctcg atgccttcgc
     5341 ctgcatcatg tgcaaccgct gccagcaggt ctgcccggcc tacaacaccg gcaaggaact
     5401 ctcccccgcc gcgctggaga tcaacaaacg ctactacatc cgccagaaca tgcccgaact
     5461 ggctgccggc gcggccaacg gcacgcccct cctcgactat gccatcagcc ccagcgccgt
     5521 ctgggcctgc acggcctgcg gccactgcat cgaaatctgc cccgtcggca acgtgcccat
     5581 gctcgacatc atggagattc gccgcgacca ggtcatgatg cagagcgagt tccccgaaca
     5641 gctcaaaggc gcgttcgtcg gcatggagcg catgggcaac ccctggcagg cccaggccag
     5701 ccgcatggag tggaccgccc ccctgccctt cgacgtgccc accgtggccg agaaccccga
     5761 ctacgaatac ctcctctggg tcggctgtgc cggcgcgttc aaccccgacg cccagaagac
     5821 cgtccaggcc ctggcgacca tcctccacac cgccggcgtc agcttcgcca tcctgggcga
     5881 cgacgagacc tgcacgggcg actcggcccg gcgggcgggc aacgaacccc tcttccaggc
     5941 cctggccgag gccaacatcg agacactcaa cgccgcccgg gccaacgagc ggcgcatcgt
     6001 gaccagttgc ccccactgct tcacgaccct gggcaaggaa tacggcgagc tgggcggcca
     6061 ctacaccgtc ttccatcaca cgcagctcat cgccgatctc gtcggcaagg ggcgcttgcg
     6121 gctagacggc ggcacgctga cccaggccac cttccacgac ccctgcttcc tggggcggca
     6181 caacggcgtc ctcgacgaac cccgcgccgc cctggccgcg gccggcatga ccctcctgga
     6241 gatgccccgc cacggcaagg attccttctg ctgtggcgcg ggcggggcgc aatactggaa
     6301 ggaggaggag cacggcacgg aggcggtcaa cgtcaaccgc tatcgcgagg cgcgggccac
     6361 gggcgcggcc gtcctggccg tcggctgtcc cttctgcaac acgatgatga gcgatgccaa
     6421 ccgcgagcag ggcgcgccga tggccgtcaa ggacgtggcc gaggtggtgg tggaggcgtt
     6481 gcgggggaag gtgggggtgg ccaactagac aaatcgcgct tccaccattg taagctttcc
     6541 tggacgaagg cgcgcaatgc aaccagcacg ctggctctgg agaagtctgc tgctctgggc
     6601 gacgatgggc aggatggtaa cagtcctgcc tgtagacgcc gccctgttga caatctaccc
     6661 gcgacaaccg ttaagcgtct ccgtggccct gcccgcggag acgttgtcgt tgccgacgcc
     6721 cgcgtcggag acgcggacgt cagagacgca ggcgtcggag acgcaggcgt ctctgctgat
     6781 taacgagatc gatgccatcg ccgcgagcga agcagccgaa ttcatcgagc tgtacgacgg
     6841 cggcgtgggc aacacgccgc tggacgggtt aacgctggtg ctctatcgcg gcgacagggc
     6901 cagcgtctat ctggccatcg gtctggacgg cgcgcgtacc gacagccgcg gctactacgt
     6961 cctcggcggc ccggccattg acgccgatca gctcttggcc gccggcctgt tgcgcgacgg
     7021 accagatgcc gtggcgctct tcgccgcgtc ggtctgggat ttccctgtca acagcccggt
     7081 ggtcgtcgaa gggctgctcg atgcggtcgt ctacgcgccc cgtgggacgc cggcgacggg
     7141 actgcaaccg ctcctgttgc cagatgaact gccggtggac gaagggcagc gcggcgcggc
     7201 cgaagcgcac tcggcccagc gtttccctaa cggccagggc caggcccgtg agatggcggc
     7261 cttcaccgcc ggcccaccga cgcccgatga acccaacaag aagtcactcg actacgcgcc
     7321 aaccgttacg aacgtctggc ccatgcccga cgccagcgac gtccccaata cgctgacgat
     7381 cagcgtcacc tttagcgagc cggtgatgct tcacgatacg cccataacgc ttgcttgcga
     7441 gcgcagcggt gtccatccct acgcgttgaa cggtggcccc ctgacctacg gctttcgccc
     7501 ctacatcgcc ctcgcgtcgg gcgatgcctg cgcggtgact gtctacggcg accgcgtcag
     7561 cgatatggac gaagacgatc caccggacgg gatgttggcg tcctacaagt ggtacttcac
     7621 ggccgcccaa accgtggccc aaaacatcgt cctcaacgaa atcgacgctg atacgccgag
     7681 caccgacaca gccgagttca ttgagctgtt cgacggcggc ggcggcgcaa cgccactgga
     7741 cgggctactg ctggtgctat tcaacggcgg cgacgaccgt agttatcgca ccatcgatct
     7801 cgccggctat acaaccgacg ccgcaggcta ctttgtgctg ggtaatgccg ccatcgtggg
     7861 agccgagata gagctgcccg acggcgcttt gcagaacggc accgacgcta tcgctctggt
     7921 ggccggccgg gccgaggcgt tccccaatgg cacgccggtg acgggggtaa cgccgttgga
     7981 cgccgtcgtc tatggccgga gcgatgagcc ggacgcgggt ctgctgcccc tgctcaacga
     8041 cggccagccg gcgattgacg agaacagccg cggcgaagcc gagagccaca gcaaccaacg
     8101 ctgccctaac ggcgctggcg gcgcgcggaa cacggccggc ttccgccaaa acacgccgac
     8161 gccgcgcgcc gccagcgatt gccagaccga tgtcgcgccg ggcgtcagtg aacgtagccc
     8221 tgcgcccggc gccacgggcg tcagtgtctc cgtaacgctg cggatcgact ttacagagcc
     8281 agtttcggtc ggcaagagtt ggctgaccct ggcctgtagc acctccggcg cgcactcatc
     8341 cacgacaacc ggcgggccga gcgccttttt catccagccg aatgagccgc tggcctatgc
     8401 cgagacgtgc acggtgacgc tgaaagccgc cctcatccac gatctggaca cggacgaccc
     8461 gccggacacg ctcaccggca atctcacctg gaaatttacg acggccgaag cgccggccgc
     8521 cttcgtcatc atcaacgaga ccgacgcgga cactcccggc agcgacacaa gcgaattcat
     8581 cgaactctat gacggtgggc gtggcgcgac acgactggat ggcctcgtgc tggtctttta
     8641 caatggggcc acgaacacag cctatacggc cctcgatctg gacggccagc gcaccgacag
     8701 cgccggctac tggttggccg gcaacgctgc tgtcacccct ggtctggcct ttgccaatgg
     8761 caccctacag aatggccccg acgccgtggc cctctatgcc ggtgacgcaa cgcagttccc
     8821 ggccgacacg cccctgcaca cgcgtggcct gctcgatgcc gttgtctatg gcgaggctac
     8881 aaatacgccc ggcctcctgg cgctactgct gcccggtgag agcgccgcgg acgaaggcgc
     8941 gcgcggagcg gccgacgcgc acgcgctgca acgctgccct aacggcgcag gcggccaacg
     9001 gcgtacggcc gcgttgcgcc ccgccccacc gacgccgggc aagaccaata tctgtgccct
     9061 tgacgccgcg cctgtcgtca ccgctgtcgt tccgtccaac ggcgagacgg gtgtggccat
     9121 tgacgtagcg ttaacggtca ctttctctga gccggtcgcg ccggtagccg gctggctgcg
     9181 cctccgttgt ggcaacaatg actatgcgat aaatgtcacg gctgctgatc cggcccgcta
     9241 cacagccacg cccacaacct tgctgcctcg cgcggcaacc tgctacgcca ctatcgccgc
     9301 cgcgcttgtc cacgacgccg acaacgacga cccgccagac attctcgcgg cggattatac
     9361 gtggtctttt gccaccgccg cgcccgtggc cgacttcgtc ctgatcaatg aactggatag
     9421 cgacacgccc ggcagcgaca cggccgagtt tgtcgagttg tacgacggcg gcgtgggcgg
     9481 gacggatctc tccggattag tactcgtctt cttcaatggc acggacgacc atgcctattt
     9541 cgccgttgat cttgatggcg tgcagacgga aggtgacggc tacgccgttg ccggaagcgc
     9601 ggcgttgggg ggcgctctaa cgctgcccgc ggccatgatc cagaatggcc ctgacgcggt
     9661 cgcgctctac gagggcgacg ccgctgactt ccccaacggc acgggcatta ccacagatgg
     9721 cctgcgcgac gccatcgtct acggcacgtc cgatccaacg gacaacggcc tgttggcgtt
     9781 gttggctgcg ggacaggccc aggtggacga gaatgcccgc gccgcggccg aagccgactc
     9841 cagcggtcgc tgtcccaacg gggccggcgg acggcgctat acggcgacct atcgccagaa
     9901 cgcgccgacg ccggggagag cgaacgactg ccacgccgac gccccgccgg ctgtcgcagc
     9961 ggtttcgccc accgatggcg ccgatcacgt ggcggcggcc gcgcagctac aggtcgaatt
    10021 tagcgagaaa gtcacgctcg atggcaactg gttcggcatt gtctgcgacg ccagtggtag
    10081 ccacacagcc acaaccgggg gtggcccgac caccttcact
//
LOCUS       DAOSMQ010000054         9958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6763_length_9958_cov_4.376855, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000054 DAOSMQ010000000
VERSION     DAOSMQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6763_length_9958_cov_4.376855"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PK829_03060"
     CDS             <1..408
                     /locus_tag="PK829_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOU40227.1"
                     /translation="DLSPQDERWLGEWARQEHGSDFLFVTGYPMVKRPFYTHPDPARP
                     AYSNSFDLLFRGTELVTGGQRLHRYDDYLAALARAGYPTAPFETYLEAFRHGMPPHGG
                     FAIGLERLLMQLLGLPNVRLATLFPRDLNRVTP"
     gene            complement(484..2664)
                     /locus_tag="PK829_03065"
     CDS             complement(484..2664)
                     /locus_tag="PK829_03065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU40228.1"
                     /translation="MAAPLRRLSLIALLLLATALRVVGLDNFPTPQGATPPGLEHDEV
                     AHWLFNRDILAGHHAIYFAEAYGHEALYHYAQAGFGALVGDHALGLRLPSAYLGVLLV
                     AVGYALGRRLFGEGVGWWSAAFLAVLFWPVFYSRLGLRAIALPVVAGLSAVAWWRAFA
                     GGQGSGGAEAIIKPSTDPLPIPLPKGEGVLSPSLWEGLGEGAAEVPLPIPLPEGEGGL
                     SPAPLLPRSSATLLLLASGALAGLSLHTYMAGRAVPIFYALYTAYLALFHRPAFRARW
                     RGVALFWLALAAVALPLVVFLLANPDAEARVSEVDAPLRALLAGDPRPVLANAAAIAA
                     GFGLRGDPLWRQGIAGRPVFDPVLGLLFYAGVVLCLWRWREPRHAFVLLWLAASVIPS
                     LVTVDAPSTIRMINALPLLLPLPLLALAAIEHRLQTRLGPSVKRKTPRLIHFGPQLST
                     LWTTLSTDLLTTLTHGLLLLLLLYHAWATVDGLWRVWPANDEVQFVWQAALTEAGAFL
                     DAAAEAGPVAIGGWTPATMDPPTMELTLRRDDLALGFFDPTNSLLLPNPAGGAARLLR
                     PAILPLAPELAALTGPWTTLPEGAAPGRQFALYRYETLPAIRPAVAADADFGGEVRFL
                     GYDPGPGCAAAEAPCTLVTYWRVLAPTGEPRRFFLHLAGPDGTPLAQDDRLGAPAEYW
                     RAGDVVVQLLTLPYTDGELRLGVYDPGDGTRLLTAGGRDYLVLP"
     gene            complement(2664..6728)
                     /locus_tag="PK829_03070"
     CDS             complement(2664..6728)
                     /locus_tag="PK829_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU40229.1"
                     /translation="MRARLGALRADILIVLSTLVLPLLLYAGVTLGPRTMIPADNLFQ
                     WAPWNTAAQQFGALPPHNGLIGDLVIQNMPWKQFALDSLRGGEIPLWNPYLFAGAPFL
                     ANGQHSMLYPFSALFLVLPLAKAYGWFALSQVWLAGVSMYAFGRILGLRRGGAALAGF
                     AYQGAQFLIISAAVFPMIVAAVVWLPLLLGCVDRVITTSLGPAARRDRALLWAAAGAV
                     ALAGQVLAGHAEFTYYTLLVMGLYALWRLAATWLAARRSGRAGADAAGAAVWLAAMVA
                     LGLLIAALQLVPLFEVGQVNFRAGSAGFDEVRGWAYPWRNAVTLLLPDFHGNPTERAY
                     RDVFTGQQVPLTVNASGQPNPRGPGTTSWGVKNYVEGAWYMGVLPLLLAAVGGAVVLR
                     RRGRPAATDAPEPTPAISAGRFFVALALVSVAFIFGTPLYALLYYGLPFIDQLHTPFR
                     WVFPLALAVAGLAGIGGDAATARRGGDGSGRFSPRRVASLLGWLALAGGGLTLLGLLL
                     SRLAYERIAGLVERVFLGLAGAADAFPSAEAFYSYQFGNVLTFGILLALAGGVLLLAR
                     RVRRLFVPAAAALIVLDLAWAGAGFHAAADPALLDFTPESVRWLQEQNPAAWRLTTYD
                     PAGSAPLNANVPWLHGLADIRGYDSIIPRQYTEYMAAIEPQNGLIYNRIQPIGSAAAL
                     ESPLLDALAVRYVVSSGPIDSPTYRLAWEGEGVRIYENLDAAPRAYTLPGAPAALPAL
                     PTAGLLAALTPATLAETRNNQVVVEATVDAPATLVLADSAFAGWRAYVRPAGSGEEAE
                     REVEITRVFGNFRGVALEPGAWTVRFRYSPRSFWLGGLMSFMGGMVLVFALVIWGWRR
                     FYRAEHTATTTRSVAKNSAAPMALSLFNKGIDFVFAAFYLRVLGPAAAGSYATAIASA
                     GIFEIVANYGLNILLIREVSQDRDHAGRFLFNSSLLRLLTGVVAVLPVAVYILAGSRG
                     PNPLSSEELTAIGLLMIGMVFSGLTLGVSGLFYVYEQAEVPAAMSTVTTLLKVGLGVA
                     ALLAGLSFVGLAAVSIVVNVVTLALLLALALSRFQLRGPWTVDRPLLGTMLRQGWPLM
                     LIHLLQTIFISIDVLLLRQMLADGERVVGYYNSAWKWFNALQIIPSYFTLALFPIISR
                     AIKQDMDAARRMYRLALRLMLLLALPTAALFTFAATPLIGLLGGQEFLPDGAIALRII
                     MWSIPFGWLNSVTNYVLIALGMERLQPRAFALAVGFNIVANVLLIPRYSYVAAAVITV
                     LSEVVLLVVFAIILRRRDAGVDWRQLTARPLLLTALMMAAMALGGRLHLLVGLALGVA
                     VYLVGLFALRVIGPEERAALGEVLPLAVTRRLGW"
     gene            complement(6728..7363)
                     /locus_tag="PK829_03075"
     CDS             complement(6728..7363)
                     /locus_tag="PK829_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HOU40230.1"
                     /translation="MSTVVLPSGYFLANRFGRLFLASLEETVGRNGLNALLNLSGRGS
                     LITAPPPDDMQLAFDTASISALLAALELVYGPRGARGLAMRAGRAMFDRLVDGFELPT
                     GFNPLAFRLLPQPTRLKIGVPALARLVTQHSDMTCRVADRGTYFEYTAERCADCWGRT
                     SKGPVCNLTVGLLLEAMSSFGRGQEFRVAQRECRAAGAAACVFHLDKEPIA"
     gene            7426..8784
                     /locus_tag="PK829_03080"
     CDS             7426..8784
                     /locus_tag="PK829_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOU40231.1"
                     /translation="MAHATMTFPPDFLWGTATAAYQVEGGNTNSDWWRWEAEGHVREG
                     SRATICCDWWANAERDLDTAAELGTNAHRLSLEWSRIEPEPSVFDGEALARYRAILQH
                     CHAAGLEPMVTLHHFSNPLWLADKGDFNSDVVVDYFGRYAAKVVDVLGDLVPKWVTIN
                     EPMTYAVMRYLTGDFPHTRRGYGAFMEGVYNLLRCHAVAYHAIKERHPAALVSTADNM
                     QVFAAPPDGTALDRWWAGAVGALYNDAWPRALHTGVFHGPLGRRRIKGLAGTADFSGI
                     NYYTRFYLRFPPRKGFIEREWGPGAVVSDGDYGEVYPYGLYQMIKRVLPYGKPIYITE
                     NGVPDAADRLRPGFILDHLRQIWQAISFCYPVMGYYHWSLVDNFEWERGWSQRFGLIA
                     LDPETQERTWRPSAHLYREICRSYTITDDMAARYAPEMLPVMWPGGETGQDSTRMTPI
                     KA"
     gene            9034..9783
                     /locus_tag="PK829_03085"
     CDS             9034..9783
                     /locus_tag="PK829_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU40232.1"
                     /translation="MSGHSKWSTIKHKKAASDAKRGKEFTRIAKELTIAAREGGGDVN
                     GNTALRLAVLKARASNMPKDNIDRAIKRGTGELEGGELIEAVYEAYGPHGVGILVHVV
                     TDNRNRAIADVRHAVSKHGGTMAEAGAVSWQFTRKGYISVSESVDPDELFMVAAEAGA
                     DDVQFNDGVAEIYSDVTAFQAVRVALEEAGVTPDEASLIYEPNNPVSLNTSGALQVMS
                     LVERIEDLDDVQDVYSTLEITDETLAAMEAA"
     gene            9801..>9958
                     /locus_tag="PK829_03090"
     CDS             9801..>9958
                     /locus_tag="PK829_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU40233.1"
                     /translation="MRILGLDPGTATTGYGIIDAADGRLTIVAYGVISTPAGDAAPDR
                     LQSIFHQLR"
BASE COUNT         1765 a         3382 c         3489 g         1322 t
ORIGIN      
        1 gacctgtcgc cgcaggacga gcgctggctg ggcgagtggg cgcggcagga gcacggcagc
       61 gacttcctgt ttgtgaccgg ctatccgatg gtcaagcggc cgttctacac ccaccccgac
      121 ccggcccgcc cggcctactc caactccttc gacctgctct tccggggcac ggaactggtg
      181 acgggcggcc agcggctgca ccgctacgac gactacctgg cggcgctggc gcgggccggc
      241 tacccgaccg cgccgttcga gacctacctg gaggcgttcc gccacgggat gccgccacat
      301 ggcggcttcg ccatcggcct ggagcggctg ctgatgcaac tgctggggct gcccaacgtg
      361 cgcctggcga cgctgtttcc gcgcgacttg aatcgcgtca cgccgtagct cagtctgtca
      421 gactgacgct ccgtagttca gtctgttaga ctgatgctct gccgctcagt ctgacagact
      481 gagctacgga agcactaaat agtcgcgccc tccagccgtc aacagccgcg tgccgtcgcc
      541 cggatcatag acacctagcc gtaactcgcc gtcggtgtac ggcagggtta gcagttgcac
      601 caccacatcg cccgcccgcc agtactcggc cggcgcgccg aggcggtcgt cctgggccag
      661 cggcgtcccg tcggggccgg ccagatgcag gaagaagcgg cgcggttcgc ccgtcggggc
      721 cagcacgcgc cagtaggtga ccagcgtgca gggcgcttcg gccgccgcgc agccggggcc
      781 ggggtcgtag cccaggaagc gcacctcccc gccgaagtcc gcatcggcgg cgacggccgg
      841 gcgaatggcc ggcagtgtct cgtagcgata gagcgcgaac tgccgccccg gcgcggcccc
      901 ttccggcagc gtcgtccacg ggccggtcag cgccgccagt tccggcgcga gggggagaat
      961 ggccggccgc agcagccgcg ccgcgccgcc ggcggggttg ggcagcagta ggctgttggt
     1021 cgggtcgaag aaacccaggg ccaggtcgtc gcgtcgcagg gtcaattcca tcgtcggcgg
     1081 gtccatcgtt gccggcgtcc agccgccgat ggccaccggc ccggcctcgg ccgcggcgtc
     1141 gagaaaggcc ccggcctcgg tcagcgccgc ctgccagacg aactgcacct cgtcgttggc
     1201 cggccagacg cgccacaggc catcgaccgt ggcccaggcg tggtagagca gcagcaggag
     1261 cagcagcccg tgggtcagcg ttgtgagcaa gtctgtggat aatgttgtcc acaatgtgga
     1321 taactggggg ccaaagtgga taagtcgagg cgtttttcgc ttgacagacg gccccagacg
     1381 tgtttgcagg cggtgttcga tggccgccag ggccagaagg ggcagcggca gcagcagcgg
     1441 cagggcgttg atcatgcgga tggtgctggg cgcgtccaca gtgaccaggc tggggatgac
     1501 cgaggccgcc agccacagca agacgaaggc gtggcgcggt tcccgccagc gccacaggca
     1561 caggacgacg ccggcgtaga acagcagccc cagcaccggg tcgaacaccg gccgcccggc
     1621 gatcccctgc cgccacagag ggtcgccgcg caggccgaag ccggcggcaa tggcggcggc
     1681 attggccagc accggccgcg ggtcgccggc cagcagtgcc cgcagcggcg cgtccacctc
     1741 gctgacgcgc gcctcagcgt cggggttggc cagcaggaag acaaccagcg gcagggccac
     1801 ggccgccagc gccagccaga acagggccac gccgcgccat cgggcgcgga aggccggccg
     1861 gtggaaaaga gccaggtagg ccgtgtagag ggcataaaag atgggcacag cgcgcccggc
     1921 catgtaggtg tggagcgaca gcccggcgag cgcaccgctg gcgagaagaa gcagggtggc
     1981 agaggagcgg gggagcaggg gtgcagggga gagccctccc tcgccctctg ggagagggat
     2041 agggagaggg acttccgccg cgccctcgcc taacccctcc cagagggagg gggacaagac
     2101 tccctcgccc tttgggagag ggatggggag agggtcggtg ctcggtttta tgatcgcctc
     2161 tgctcccccg cttccctgcc ccccggcaaa agcccgccac caagccaccg ccgacaagcc
     2221 cgccaccacc ggcaaagcga tggcccgtag ccccaggcgg ctgtagaaca ccggccagaa
     2281 gaggacggcc aggaaggcgg ccgaccacca gccgacgccc tcgccgaaca gccgccgccc
     2341 cagggcgtag ccgacggcca ccagcagcac gcccaggtag gccgacggga gccgcaagcc
     2401 cagggcgtga tcgcccacca gcgcgccgaa gccggcttgc gcatagtgat agagggcctc
     2461 gtggccatag gcttcggcga agtagatggc gtggtggccg gccaggatgt cgcggttgaa
     2521 cagccagtgg gcgacctcgt cgtgctccag gccgggcggc gtcgcccctt gtggcgtggg
     2581 gaagttgtcc agcccgacga cgcgcagcgc cgtggccagc aggagcaggg cgatcagcga
     2641 gaggcggcgc agcggcgcgg ccatcaccat cccagccgcc gcgtcacggc cagcggcagc
     2701 acctcgccca gggccgcgcg ctcctccggg ccgatgacgc gcagggcaaa gaggccgacc
     2761 aggtagacgg cgacgcccag cgccagccca acgagcaggt gcagccgccc gcccagcgcc
     2821 atcgccgcca tcatcagcgc cgtcagcagc agcggccggg ccgtcagttg ccgccagtcc
     2881 acgcccgcgt cgcgccggcg caggataatg gcgaagacga ccagcagcac cacctccgat
     2941 agcacggtga tgaccgccgc ggcgacgtag ctgtagcgcg ggatgagcag gacgttggcg
     3001 acgatgttga agccgacggc cagcgcgaac gctcgcggct gcaagcgctc catgcccagg
     3061 gcgatgagga cgtagttggt gacgctgttg agccagccga agggaatcga ccacatgatg
     3121 atccgcaagg cgatagcccc gtcgggcagg aactcctggc cgcccagcag accgatcagc
     3181 ggcgtggccg cgaaggtgaa cagcgccgcc gtgggcaggg ccagcagcag catcagccgc
     3241 agcgccagcc ggtacatgcg ccgcgccgcg tccatgtcct gcttgatggc ccggctgatg
     3301 atcgggaaga gggccagggt gaagtaggac gggataatct gcagggcgtt gaaccacttc
     3361 caggcgctgt tgtagtagcc gacaacgcgc tcgccgtcgg ccaacatttg ccgtagcagc
     3421 agcacgtcga tggagatgaa gatcgtctgc aaaaggtgga tgagcatcag cggccagccc
     3481 tggcgcagca tcgtgcccag cagcggccga tccactgtcc atggcccgcg cagctggaag
     3541 cggctaaggg ccagcgccag cagcagggcc agggtgacga cgttgacgac gatcgacacc
     3601 gccgccagcc cgacgaagct gaggccggcc agcagcgcag ccacgcccag gccgacctta
     3661 agcagcgtcg tcactgtgga catggccgcg ggcacttcgg cctgctcgta aacgtagaac
     3721 aggccggaca cgcccaacgt cagcccggag aagaccatgc cgatcatcag caggccaatg
     3781 gcggtcagct cctcgctgct gagcgggttg gggccacggc tgccggccag gatgtagacg
     3841 gccacgggca gcacggccac gacgcctgtc agcagccgca gcagactgga gttgaacagg
     3901 aagcggccgg cgtggtcgcg gtcttgcgac acttcgcgga tgagcaggat gttgaggccg
     3961 tagttggcga cgatctcgaa gatgccggcc gaggcgatgg ccgtggcgta gctgccggcc
     4021 gccgccgggc cgagcacgcg caggtagaac gcggcgaaga cgaagtcgat gcccttgttg
     4081 aacaggctca gcgccatcgg cgcggcgctg ttcttggcca cgctgcgcgt cgtcgtggcc
     4141 gtgtgctcgg cgcggtaaaa gcgccgccag ccccagatga ccagggcaaa gaccagcacc
     4201 atgccgccca tgaacgacat cagcccgccg agccagaagc tgcgcgggct atagcggaag
     4261 cgcaccgtcc acgcgcccgg ctccagggcc acgccgcgga agttgccgaa gacacgtgtg
     4321 atttcgacct cgcgctcggc ctcctcgccg ctgccggccg ggcgcacgta ggcccgccag
     4381 ccggcaaagg cgctgtcggc cagcaccagc gtggccggcg cgtccacggt cgcctcgacg
     4441 acgacctgat tgttgcgcgt ctcggccagc gtcgccgggg tcagggcggc caggaggccg
     4501 gccgtgggca gggccggcag cgccgccggc gcgccgggca gcgtgtaggc gcgcggcgcg
     4561 gcgtcgaggt tctcgtagat gcgcacgccc tcgccctccc aggccaggcg gtaggtgggc
     4621 gagtcgatag ggccgctgct gacgacgtag cgcaccgcca gcgcgtcgag cagcggcgac
     4681 tccagcgccg cggccgagcc gatgggctgg atgcggttgt agatcagccc gttctgcggc
     4741 tcgatggcgg ccatgtattc ggtgtactgg cgcgggatga tggagtcgta gccgcggatg
     4801 tcggccaggc cgtggagcca ggggacgttg gcgttgagcg gcgcgctgcc ggcggggtcg
     4861 taggtcgtta gccgccacgc ggccgggttc tgctcttgca gccagcgcac cgactccggc
     4921 gtaaagtcga gcagtgctgg atcggccgcg gcgtggaagc ccgcgccggc ccaggccagg
     4981 tcgaggacga tgagcgccgc ggcggcgggc acgaagagcc gccgtacacg ccgcgccagc
     5041 agcaggacac ccccggccag agccagcagg atgccgaagg tgagcacgtt cccgaactga
     5101 tagctataga atgcctcggc cgaggggaaa gcgtcggccg cgccggccag ccccaggaag
     5161 acgcgctcca ccagaccggc gatgcgctca taggccagcc ggctgagcag caagcccagc
     5221 agcgtcagcc cgccgccggc cagcgccagc catcccagca acgaggccac ccggcgcggc
     5281 gagaagcggc ccgaaccgtc gccgccgcgc cgcgcggttg ccgcgtcgcc gccaatgccc
     5341 gccagtccag ccacggccag ggccagcggg aagacccagc ggaagggcgt gtgtagctgg
     5401 tcgatgaacg gcaggccgta gtagagcagg gcgtagagcg gcgtgccgaa aatgaaggcc
     5461 accgagacga gagccagggc gacgaagaag cggccggcgg agatagccgg cgtcggctcc
     5521 ggcgcgtctg ttgcggcggg ccgtccgcgc cgccgcaaga ccaccgcgcc gccgacggcc
     5581 gccagcagca agggcagcac gcccatgtac cacgccccct cgacgtagtt cttcacgccc
     5641 caactggtcg tgcccgggcc acggggattg ggctggccgc tggcgttgac ggtcaggggc
     5701 acctgctggc cagtgaagac gtcgcggtag gcgcgctcgg tggggttgcc gtggaagtcg
     5761 ggcagcagca gcgtcacggc attgcgccag gggtaggccc agccgcgcac ttcgtcgaag
     5821 ccggccgacc cggcgcggaa gttgacttgc cccacctcga acaggggcac cagttgcagc
     5881 gcggcgatga gcagccccag ggcgaccatt gccgccagcc agacggccgc cccggcggcg
     5941 tctgcgccgg cgcgcccgct acgccgcgcc gccaaccacg tagctgccag ccgccacagc
     6001 gcatacagcc ccatgaccag cagcgtatag taggtaaact cggcgtgccc ggccagcacc
     6061 tggccggcca gagcgaccgc cccggccgcg gcccaaagca aggcgcggtc gcgccgcgcc
     6121 gccgggccga ggctggtggt gatgacccga tcgacgcagc ccagcagcag cggcagccag
     6181 acgacggcgg cgacgatcat gggaaagacg gcggcgctga tgatcaggaa ctgcgccccc
     6241 tgataggcaa agccggccag ggccgcgcca ccccgccgca agccgaggat gcggccgaag
     6301 gcgtacatgc tgactccggc cagccacacc tggctgagag cgaaccagcc gtaggccttg
     6361 gccagcggca agacgaggaa cagggcgctg aacgggtaga gcatggagtg ctgcccgttg
     6421 gccaggaagg gtgcgccggc gaacaggtag gggttccaca gcggaatttc gccgccgcgc
     6481 aggctgtcga gggcgaactg cttccagggc atgttctgga tgacgaggtc gccgataagg
     6541 ccgttgtggg gcggcagcgc gccgaactgc tgcgcggcgg tgttccaggg cgcccactgg
     6601 aagaggttgt cggccgggat catcgtccgc gggccgaggg tgacgccggc ataaagcagt
     6661 agcggcagca ccagggtgct caggacgatg aggatgtcgg cgcgcaacgc gcccaagcgg
     6721 gcacgcatca tgcgatcggc tctttgtcca ggtggaagac acaggccgcg gccccagcgg
     6781 cgcggcactc ccgctgggcg acgcggaact cctggccgcg gccgaagctg ctcatcgctt
     6841 ccagcaacag accgacggtc agattgcaga ccggcccctt gctcgttcgc ccccagcagt
     6901 cggcgcagcg ctcggcggtg tactcgaagt atgtgccgcg gtcggccaca cggcaggtca
     6961 tatcgctatg ttgcgtcacc agacgggcca gggccggaac gccgattttg agccgcgtcg
     7021 gctgtggcag cagccggaag gccagcgggt tgaagcccgt cggcagctcg aagccgtcca
     7081 caaggcggtc gaacatggcc cggccggcgc gcatggccag cccacgcgcc ccgcgcggcc
     7141 cgtagaccag ctccagcgcg gccagcagcg cggagataga ggccgtgtcg aacgccaact
     7201 gcatgtcgtc cggcggtggg gcggtgatca ggcttccccg gccactcaga ttcagcagcg
     7261 cgttcagccc gttgcggccg accgtttctt cgagcgaggc gaggaacagg cggccgaagc
     7321 gattggcgag gaagtagccg ctgggcagga cgacagtact cacaagggcc aagtatagga
     7381 gtctcgcgcc gggacgcaaa gaacggtatc agctatactt tgcccatggc gcacgcaacg
     7441 atgacctttc cacccgactt cctttggggc acagccaccg cggcctatca ggtcgagggc
     7501 ggcaacacca acagcgattg gtggcgctgg gaggccgagg ggcatgttcg cgagggcagt
     7561 cgcgccacga tttgctgcga ctggtgggcc aacgccgagc gcgacctgga cacggccgcc
     7621 gagttgggca ccaatgccca tcgcctgtcg ctggagtgga gccgcatcga gccggagccg
     7681 agcgtcttcg acggcgaagc cctggcccgc taccgcgcca tcctccagca ctgtcacgcc
     7741 gccggcctgg agccgatggt caccctccac cacttcagca acccgctctg gctggccgac
     7801 aagggcgact tcaacagcga tgtcgtcgtc gactacttcg gtcgctacgc cgccaaagtg
     7861 gtggatgtgc tcggcgacct ggtgcccaaa tgggtcacca tcaacgagcc gatgacctac
     7921 gccgtcatgc gctacctgac cggtgacttc ccccatacgc gccgcggcta tggcgcgttc
     7981 atggagggcg tctacaacct cctgcgctgc cacgccgtcg cctaccacgc catcaaggag
     8041 cgccatccgg cggcgctggt cagcacggcc gacaacatgc aggtcttcgc cgccccgccc
     8101 gacggcacgg ccctcgaccg ctggtgggcc ggcgcggtgg gcgcgctcta caacgacgcc
     8161 tggccccggg cgctgcacac cggcgttttc cacgggccgc tgggccggcg gcggatcaag
     8221 gggctggcgg gcacggccga tttcagcggc atcaactact acacccgctt ctacctgcgc
     8281 ttcccgccgc ggaagggatt catcgagcgg gagtgggggc cgggcgcggt ggtcagcgac
     8341 ggcgactacg gcgaggtcta cccctatggc ctttaccaga tgattaagcg cgtgttgccc
     8401 tatggcaagc ccatctacat caccgagaac ggcgtgcccg atgccgccga ccggctgcgg
     8461 ccgggcttca tcctcgacca cttgcgccaa atctggcagg cgatcagctt ttgctacccg
     8521 gtcatgggct attaccactg gtcgctggtg gacaacttcg agtgggagcg cggctggtcg
     8581 cagcgcttcg gcctgatcgc cctcgacccg gagacgcagg agcggacatg gcggccgagc
     8641 gcccacctct accgcgagat ttgccgcagc tacaccatta ccgacgacat ggccgctcgc
     8701 tacgcgccgg agatgttgcc ggtcatgtgg ccagggggag agacaggcca ggatagcacg
     8761 cggatgacgc cgatcaaagc gtagcggtct tgctgacagg gctttgccaa agtcgtttgg
     8821 cgagcattga ccttgaccgg tggacggcca gtcgtcctct ggtggcgggg ccaggcgcgg
     8881 ccgcccaccg gtcaggtcgg tgtgcctggg gtgactttgg caaagtcctg atagcgtctc
     8941 ttgacgcgct gtcaccgcgc cttctttgtg ctataattca gggcgctaac cgcacaaatc
     9001 ccaacgcatc ccttgcgtat tggagaaagg aacatgtccg gacactcaaa atggtccacc
     9061 atcaaacaca agaaggcggc cagcgacgcc aagcgcggca aggaattcac gcgcattgcc
     9121 aaggagctta ccattgccgc gcgcgaaggg ggcggcgacg taaacggcaa cacggccctg
     9181 cgtctggccg tcctcaaggc ccgcgcctcg aacatgccca aggacaacat tgaccgggcc
     9241 atcaagcgcg gcacgggcga gttggaaggc ggcgagctga tcgaggccgt ctacgaagcc
     9301 tatggccccc acggcgtcgg catcctcgtc cacgtcgtca ccgacaaccg caaccgcgcc
     9361 attgccgacg tgcgccatgc cgtcagcaag cacggcggca cgatggccga ggccggcgcg
     9421 gtgtcgtggc agttcacgcg caagggctac atcagcgtct ctgaaagcgt cgatcccgat
     9481 gaactgttca tggtcgccgc cgaagcgggc gcggatgacg tgcagttcaa cgacggcgtg
     9541 gccgaaatct attccgacgt gaccgccttc caggccgtgc gcgtcgccct tgaagaggcc
     9601 ggtgttaccc ccgatgaggc ttcactgatc tacgagccga acaaccctgt cagcctgaac
     9661 acgtcgggcg cgctgcaagt gatgagcctg gtggagagga tcgaggacct cgacgacgtg
     9721 caggacgtct actccacgct agagatcacc gacgagaccc tggcggcgat ggaagcggcc
     9781 tagcgcgggc gtgttcgtcc atgcgcatcc tggggcttga tcccggcacg gccacaaccg
     9841 gttacggcat catcgacgcc gccgacggcc gtctgactat cgttgcctac ggcgtcatca
     9901 gcacgccggc cggcgacgcc gcccccgacc gtctgcaatc catttttcat caacttcg
//
LOCUS       DAOSMQ010000055         9886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6829_length_9886_cov_4.214729, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000055 DAOSMQ010000000
VERSION     DAOSMQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6829_length_9886_cov_4.214729"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK829_03095"
     CDS             <1..190
                     /locus_tag="PK829_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40234.1"
                     /translation="SDAVLDVVPQDREVLLEAMRLKQFRGYCDGGQRAFAWPTAEHGD
                     VRLVVSWRTLRQSESCDE"
     gene            231..1940
                     /locus_tag="PK829_03100"
     CDS             231..1940
                     /locus_tag="PK829_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40235.1"
                     /translation="MGQMHSDRIILVLGGAGLVGAQIVREIARELEPERIIVASLYRG
                     EVREFLHDARREFCNVDFVGAWGDVFVRDEFMEDNRRRLIGTRSRRDSLYQDLYGGLD
                     DAYARSGLVQLIRRYRPNVIVDCINTATAISYQDSDSQSKRVHDLLEQLNQAVDHQDL
                     TALAGLGRDVEVNVSSLIISQSLPQLIRHVQLIHRAMTEVGTRLYLKVGTTGTGGMGL
                     NIPYTHSEDRPSFKLMSKTAVAFAHTGLMFLMARTPDGPLVKELKPAAMIGYRRVAYK
                     TVKLRGRPQMRYQSVEQPLGQRLLLRGDETQYERLDKLHMAGVDTGENGFFARGEFET
                     ITHTDQMEFITPEEIAEQAVLEILGSNTGYDVIAGIDSSVLTPSYRAGVLRQAALEKL
                     ARIEEETGSHSVALGQLGPPQLSKLLYEAHLLKLNYGTLSAVLAAAPEEIAQTLFDYL
                     RRDDILRTTITSIGVPILTPGGDCLIRGPRLNIPESIYHEIDATPAHVDAWATKGWVD
                     LRPGNFRLWHDRFERMQRTKHMLHTRGTSSVTMKTYLPETIEIGAIVAWLFINEDSGY
                     RIK"
     gene            2051..3142
                     /locus_tag="PK829_03105"
     CDS             2051..3142
                     /locus_tag="PK829_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40236.1"
                     /translation="MQLSKDFTGLTMPVFSAFGWAGEENALKYALSQMQLFIEALYLR
                     LPADIRDDFATYGLSAENQNVYLATGDTYDKEAFIAFNARPMSLEVQLGIVGQNVLSK
                     GLAAINKDPAATYHVLTQLDPSWTLRVQQMQIDAEAGERAHHLDLYKDSVNNLTEAQA
                     REIFERTAYLNEEDKWVTPVYLSLRLPSERVAAMGLAVLDIARDLVVAVVPSLRLFTG
                     RKAKKARAAHTSKARAPRVEPTEETPAGEPTITGSIKALADSFSYVADLKPLHIRRGF
                     INLTPAHWPFFAASSRSETREVTVVFGGRQDKHSSVWRLQPDDQARLVLGQPVHEWLE
                     ENFATSDTIRILARRLDNDEIRITLEPAP"
     gene            3161..4567
                     /gene="pyk"
                     /locus_tag="PK829_03110"
     CDS             3161..4567
                     /gene="pyk"
                     /locus_tag="PK829_03110"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOU40237.1"
                     /translation="MARTKIVATIGPGSAHSETIRRMLAAGMTVARVNFSHGDHAAHI
                     KTVEMLREVARDEGKPLAILGDLQGPKLRLGHVKAGGMMLSPGDEIVLTPHPGQPAMI
                     HLPHPDLIAAVQPGSRLVIGDGEVEFTVAEKRGDTLRVIVTVAGLLESRKGVNAPGTE
                     LPISCLSDKDREDLELICDLDFDYIALSFVRTAYDVEELRALMKNRPRQIPIIAKIEK
                     SEAIENLMAIRDAADGMMVARGDLGIEMPPQELPLLQKRIIAACNDAGKPVITATQML
                     QSMVEHPRPTRAEASDVANAILDGTDAVMLSGETATGKYPVESVLMMKQIAEIMERAF
                     PYDQWRRSHLVPHFEGDTTEAISDASCTIAEEIGAAAIVSTTLSGWTAQQVARFRPRS
                     PILAVSPSAYTQRRLALVWGVETLLVPDFEDTDAMLQQTIQAVRQSGLAPGQRVVITG
                     GVPFGKSGQTNLIKVHTI"
     gene            4669..5406
                     /locus_tag="PK829_03115"
     CDS             4669..5406
                     /locus_tag="PK829_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOU40238.1"
                     /translation="MSWLTADRPLIIGHRGACADAPENTLAAFALALEQNADGVELDV
                     QLSADGAPIIMHDATVDRTCDGVGRVEDMTLVELRQLTIDNDHPVPALDDLFALLGRR
                     TLYNVELKALGLDDGGLAEAVARCVVAHRVADCVLVSSFSPFAVRSARRHLPAAVPVA
                     LLRERRATRVFHRVLRTAADHPDYQLVDEALMAWARRKGLHVHAWTVNDVAEGRRLTG
                     LGVHGIITNTPGVMRAALDAPAHAEGG"
     gene            5410..6294
                     /locus_tag="PK829_03120"
     CDS             5410..6294
                     /locus_tag="PK829_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU40239.1"
                     /translation="MRVKVILNPYANRWRAGAQAGATAEAFRAAGVDCDLVTTNAPRH
                     GDALAEAAARGGYDAVVAAGGDGTINEVINGLLRAAGDGPTKPFGIVPLGTANDFNLM
                     AGLPSVPAESVAVIARGQTRLIDAGQVNERFFINNSAAAMEPMVTLENIKMKRLSGEV
                     RYIVALLRALAKLKPWQMSLAWDGGGYEGPAYLLSVCNSARTGGFMMAPGALLDDGLL
                     DMVFAPQVSKGQVISILLKLMRGQHIQHPAVTFRRVTAIDLTSRPGTPLHSDGEIFAE
                     AAEVVRYRVLPGRVRLLS"
     gene            6346..7047
                     /locus_tag="PK829_03125"
     CDS             6346..7047
                     /locus_tag="PK829_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40240.1"
                     /translation="MLTADELVEGIFGGQHLALRAEFAGWVRGSRRFAAFADVYRGKI
                     RAKLRHARDEGGLRDVRAELVTAALLLREDRFSLEYEKYAAARTRGPDFTVTFKGHMP
                     FNVEVRRIRNGELDTADAGARTAKLIAVLVDKASQMPPSIINVLWLSAEGEIDADDLT
                     HAVATVRQLAERKDDDYFARRGYRDAADFLGQYQRLSGIVRPAAGAPVLWRNPTARHK
                     IPPDLAAALGRLRGP"
     gene            7097..7274
                     /gene="ssrS"
                     /locus_tag="PK829_03130"
     ncRNA           7097..7274
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK829_03130"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(7463..8167)
                     /locus_tag="PK829_03135"
     CDS             complement(7463..8167)
                     /locus_tag="PK829_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOU40241.1"
                     /translation="MPQIDSLDRGGSQQRDSLSHQAIEDYVKTIYALAQEESPVSTSR
                     VAAARMVKPASATSMIQRLARLNLVNYEKHYGVTLTAAGEKLALEVIRHHRLLELYLM
                     EALGFRWDEVHEQADILEHVISEKLEERIAAALNHPTFDPHGEPIPALDGTMPVVDTT
                     ALSDVPAGARARISRVPDDANSELLRYLAGLGLVPGAEVLVAEVAPFLGPLTVEIDGM
                     RHAIGRNLADIVLVEK"
     gene            complement(8218..9363)
                     /locus_tag="PK829_03140"
     CDS             complement(8218..9363)
                     /locus_tag="PK829_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU40242.1"
                     /translation="MDWEPVLAECIAFAQRLIRTPSMPGQEAALAALVAEELRRLGYD
                     EVWGDAAGNVYGRLHGVDRSLPALVLNTHLDHVDPGDLSLWSVPPFAAEIDGDRLIGR
                     GSCDIKGPLAVQVYAGAALRRAGQRPRRDVVFCGVVEEEVGGAGAIYWAEHLDYPVEL
                     VVLGEPSSNTLALGHRGILQLWLTFHGRSVHASAPERGLNPNYALAAFLGGLEAAVAA
                     LPAHPILGPTSVAPTILQVDTTSGNVTPAWARVLLDFRTATESVDTLAALIDRLAAGA
                     PHTLAYAEGLEPMAGSSAPLAGFYTPPDSPLVARTAAAIAAGMGRPPELTRYRFATDG
                     RHFAPHGLAVVGYAPGEEHMAHTAGESISLNQMAESLRGYVALLRDF"
     gene            complement(9389..>9886)
                     /locus_tag="PK829_03145"
     CDS             complement(9389..>9886)
                     /locus_tag="PK829_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40243.1"
                     /translation="NIGTIMGGTVVNRVPHQASAGVEMRAYDPVVFEEGVGRVLALNE
                     ACTVRSAYDGYPCKVTIDIEGRWQPWPPNAGTMALFEVWSAAAAELGLGTVSLQSRGG
                     LSDGNWLWDSLPTIDGLGPEGYNAHSSQRSDDGSKDQEYVLASSFVPKAVLDAAAIVR
                     LLGAA"
BASE COUNT         1721 a         3264 c         3260 g         1641 t
ORIGIN      
        1 ctccgacgcc gtactggatg tcgttccgca ggatcgcgag gtgctgctgg aggcgatgcg
       61 gctgaagcag ttccgcggct attgtgatgg gggccagcgg gcgtttgcgt ggccgacggc
      121 cgagcatggt gatgtccgcc tagttgtcag ttggcgcacc ttgcgccaat ccgaatcgtg
      181 tgacgagtag ccgcgcccgg cgtgggccgc ggcctgtttc gtgaggaggt atgggtcaga
      241 tgcattccga ccggatcatc ctagttctcg gtggcgccgg ccttgtcggc gcgcagatcg
      301 tccgcgagat cgcccgcgag ttggagccgg agcgcatcat cgtcgccagc ctctaccgcg
      361 gcgaagtgcg cgagtttctc cacgacgccc ggcgcgagtt ctgcaacgtc gatttcgtcg
      421 gcgcgtgggg cgatgttttc gtgcgcgacg agttcatgga agacaaccgc cgccggctga
      481 tcggcacgcg cagccgccgc gacagcctct atcaggacct ctatggcggg ctggacgacg
      541 cctatgcccg ctccgggctt gtccagctca tccgccgcta ccggcccaat gtcatcgtcg
      601 attgcatcaa caccgccacg gccattagct accaggacag cgacagccag agcaagcgcg
      661 tccatgacct gctggagcag ctcaaccagg ccgttgacca ccaggacctg accgccctgg
      721 ccggcctcgg ccgcgacgtg gaagtcaacg tcagttcgct gatcatctcc cagtcattgc
      781 cgcagctcat tcgccatgtc cagctcattc accgggcgat gacggaggtc ggcacgcgcc
      841 tctatctaaa ggtcggcacg acgggcacgg gcggcatggg cctgaacatc ccctataccc
      901 atagcgagga tcggcccagc tttaagctga tgagcaagac ggccgtcgcc ttcgcccaca
      961 ccgggctgat gttcctgatg gcgcgcacgc ccgacgggcc actggtgaag gagctaaagc
     1021 cggcagcgat gattggctac cggcgtgtgg cctacaagac ggtcaagctg cgcgggcggc
     1081 cacaaatgcg ctaccagagt gtggagcaac ccctgggcca gcgcctgctg ctgcgcggcg
     1141 acgagaccca gtatgagcgc ctggacaagc tgcacatggc cggcgtcgac accggcgaga
     1201 acggtttctt cgcccgcggc gaattcgaga cgattaccca caccgaccaa atggagttta
     1261 tcaccccgga ggagatcgcc gagcaggcag tgctggagat attgggcagc aacaccggtt
     1321 acgacgtgat cgccggcatc gacagcagcg tgctgacgcc cagctaccgc gccggggtgc
     1381 tgcgccaggc ggcgctggag aaactggcgc gcattgagga agagacgggc agccacagcg
     1441 ttgccctggg ccagctcggc ccgccgcagt tgtcgaagct gctctatgag gctcacttgc
     1501 tgaagctcaa ctatggcacg ctcagcgctg tcctggccgc cgcgccggag gagatcgccc
     1561 agacgctgtt cgactatctg cgccgcgacg acatcctgcg cacgaccatc acctccatcg
     1621 gcgtgcccat cctgacgccc ggtggcgact gcctgatccg cgggccgcgc ctgaacattc
     1681 cggagagcat ctaccacgag atcgacgcca ccccggccca cgtcgatgcc tgggcgacca
     1741 agggctgggt agacttgcgg ccgggcaact tccgcctctg gcacgaccgc ttcgagcgca
     1801 tgcaacgcac caagcacatg ctccacacgc gcggcacgtc gtcggtgacc atgaagacct
     1861 atctaccgga gaccatcgag atcggcgcga tcgtggcctg gctattcatc aatgaggaca
     1921 gcggctatcg catcaagtaa gcttgacatt tggcccacct gggctaggat caatccctga
     1981 cgatcgccgt aattccccac acgctttgtg tacccaccgg cccggcgcag ttgttcacca
     2041 gagttatgct atgcaactat ctaaagattt taccggcctg accatgcccg tcttttcagc
     2101 ctttggctgg gctggcgaag aaaacgccct gaagtatgcc ctgtcgcaga tgcagctgtt
     2161 catcgaggcg ctctatttgc gcctgccggc cgacatccgc gacgatttcg ccacctatgg
     2221 actgagcgcg gagaaccaga acgtctacct ggccaccggc gatacgtatg acaaggaggc
     2281 gttcatcgcc ttcaacgccc gccccatgag cctggaagtg cagctgggca tcgttggcca
     2341 aaacgtactg tcgaaggggt tggcggccat caacaaggac cccgccgcca cctaccatgt
     2401 gctcacccag ctagacccat cgtggacgct gcgcgtgcag cagatgcaga tcgacgccga
     2461 ggcgggcgag cgcgcccatc acctcgacct gtataaggac agtgtcaaca acctgaccga
     2521 agcccaggcg cgagagatct ttgagcgtac tgcctacctc aacgaagagg acaagtgggt
     2581 gacgcccgtc tacctgagcc tgcggctgcc gtcggagcgc gtcgcggcga tggggctggc
     2641 ggtgctggac atcgcccgcg atctggtggt cgccgttgtg cccagcctgc gcctcttcac
     2701 cggccgcaag gcgaaaaagg cacgcgccgc ccacacgtct aaggcccgtg cgccgcgtgt
     2761 cgagccgacg gaggagaccc cggccggcga gccgacgatc accggcagca tcaaggccct
     2821 ggccgatagt ttcagctacg tcgctgacct gaagccgctc cacatccggc gaggcttcat
     2881 caacctgacg ccggcccact ggcccttttt cgctgccagc agccgctccg agacgcgcga
     2941 agtgaccgtg gtctttggcg ggcggcaaga caagcacagt agcgtctggc ggttgcaacc
     3001 cgacgaccag gcccgcctgg ttctggggca gccggtccac gaatggttgg aagagaactt
     3061 cgctacgagc gacaccatcc gcatcctggc gcgacggctc gacaacgacg agatccgtat
     3121 caccctcgaa ccagccccct aaaccgcccg gaacgaaagc atggcgcgca cgaaaatcgt
     3181 ggcaacgatc gggccgggtt cggcccacag cgagaccatt cgccggatgc tggctgcggg
     3241 catgaccgtc gctcgcgtca acttctctca cggcgaccac gccgcccaca tcaagacagt
     3301 tgagatgctg cgcgaggtgg cgcgcgacga gggcaagcca ctggccatcc tgggcgactt
     3361 gcaggggccg aagctgcgcc tgggccacgt caaggccggc ggtatgatgc tctcgccggg
     3421 cgacgagatc gtgctgacgc cccaccccgg ccaaccggcc atgatccacc tgccgcaccc
     3481 cgacctgatc gccgctgtgc agccgggcag ccgcctggtc atcggcgacg gcgaagtcga
     3541 atttactgtg gccgagaagc gcggcgatac gctgcgcgtc attgtcactg tggccgggct
     3601 actagagtcg cgcaagggcg tcaacgcccc cggcaccgaa ctgcccattt cctgcctgtc
     3661 ggacaaggac cgcgaagatc tagaactcat ctgcgacctg gactttgact atatcgccct
     3721 gtcctttgtg cgcacggcct atgatgtgga agagttgcgc gcgctgatga agaaccggcc
     3781 gcggcagatt cccatcatcg ccaagatcga gaagagcgaa gccatcgaga atctaatggc
     3841 cattcgcgac gcggccgacg gtatgatggt cgcccgcggt gacctgggca tcgaaatgcc
     3901 gccccaggag ctgccgctgc tacaaaagcg catcattgcc gcctgcaacg atgccggcaa
     3961 gccggtcatc acggccacgc agatgctgca atccatggtc gagcatcccc ggccgacgcg
     4021 ggccgaggcc agcgacgtgg ccaacgccat cctcgacggc accgacgcgg ttatgctctc
     4081 cggcgagacg gccacgggca agtacccggt tgagtcagtg ctgatgatga agcagatcgc
     4141 cgagatcatg gagcgcgcct tcccctatga ccaatggcgg cgcagtcatc ttgtgcccca
     4201 ctttgagggc gacaccactg aggccattag cgacgccagt tgcaccatcg ccgaggagat
     4261 cggcgcggcg gccatcgtca gcaccacgct cagcggctgg acggcgcagc aagtggctcg
     4321 cttccgtccg cgcagcccca ttctggccgt ctctccatcg gcctacacac aacggcggct
     4381 ggcgctggtg tggggtgtgg agacgctgct ggtgcccgac ttcgaggaca ctgacgcgat
     4441 gttgcagcaa accattcagg ccgtgcgcca gtcggggctg gcaccggggc agcgcgtggt
     4501 catcaccggc ggcgtgccct tcggcaaatc tggccagacc aacctcatca aggttcatac
     4561 catctaatca attaggaatt acgaatgagg aattagggat gaaaaaccgc cgggccattg
     4621 gctcttcatt cttaattcct cattcgtaat tcctcattcc tcattctcat gtcctggctg
     4681 accgccgacc gcccgctcat catcggccat cggggcgcct gtgccgacgc gccggagaac
     4741 accctggccg cctttgccct ggcgctggag cagaacgccg acggcgttga actggacgtg
     4801 cagctgtcgg ccgacggcgc gccgatcatc atgcacgacg ccaccgttga ccgcacctgt
     4861 gatggcgtcg gccgggtcga ggacatgacc ctggtcgaac tgcgccagct gaccatagac
     4921 aacgaccacc ccgtgccggc gctggacgac ctgttcgcgc tgctcggccg gcgcacactc
     4981 tacaacgtcg agctaaaggc gctggggctg gacgacggtg ggctggccga ggcggtcgct
     5041 cgctgtgtcg tcgcccaccg ggtggccgac tgcgtgctgg tgtcgtcttt cagcccgttc
     5101 gccgtccggt cggcgcggcg gcatttgccg gcggccgtgc ccgtggccct gttgcgcgaa
     5161 cgccgggcga cgcgcgtctt ccaccgcgtg ttgcgaacgg cggccgacca tcccgactat
     5221 caacttgttg atgaggcgct catggcctgg gcgcgacgga aggggctgca cgtccacgcc
     5281 tggacggtca acgacgtggc cgaggggcgg cggctgaccg gcctgggcgt ccacggcatc
     5341 atcaccaaca cccccggcgt catgcgggcg gccctggacg cgccggccca tgcggaagga
     5401 ggttgatcca tgcgcgtcaa agtcatcctc aacccctatg ccaaccgctg gcgggccggg
     5461 gcgcaggcag gggccacagc cgaggccttc cgcgccgccg gcgtcgattg tgacctggtg
     5521 acgaccaacg ccccgcgcca cggcgacgct ctggccgagg cagcggcccg cggtggctat
     5581 gatgccgtcg tcgccgccgg aggtgacggg acgattaacg aggtcatcaa cggcctgctg
     5641 cgcgccgccg gcgacgggcc gaccaagcca ttcggcatcg tgccgctggg cacggccaac
     5701 gacttcaacc tgatggccgg gctgccgtcg gtgccggccg agagcgtggc cgtcatcgcc
     5761 aggggacaga cgcggctgat cgacgccggg caggtaaacg aacgcttttt catcaacaac
     5821 agcgccgcgg cgatggaacc gatggtgacg ttggaaaaca tcaagatgaa gcggctgtcg
     5881 ggcgaggtgc gctacatcgt tgccctgcta cgggcgctgg ccaagttaaa gccgtggcaa
     5941 atgtcgctgg cctgggacgg tggcggctat gaggggccgg cctacctgtt atcggtctgc
     6001 aacagtgctc gcacgggcgg cttcatgatg gcccccggcg cgctgctgga tgacggcctg
     6061 ctggatatgg tcttcgcgcc gcaggtatcg aagggacagg tgatcagtat cctgttgaag
     6121 ctgatgcgtg gccagcatat ccagcatcca gccgtcactt tccgccgcgt gacggccatc
     6181 gacctcacca gccggccggg cacgccgctg cacagcgacg gcgaaatctt cgccgaagcg
     6241 gccgaggtcg tgcgctatcg ggtgttgccg gggcgggtgc ggttgctaag ctagtggtcg
     6301 gaaccagcgg gtcagtgatt ggtgggccgc ggtcagtaat caacagtgct aacggccgac
     6361 gaactcgttg agggcatctt tggtgggcag catctggcgc tgcgagccga gtttgccggg
     6421 tgggtgaggg ggtcgcggcg gtttgcggcc tttgccgacg tttaccgcgg caagattcgc
     6481 gccaagctgc gccatgcccg cgatgagggc ggcctgcggg acgtgcgcgc cgagttggtc
     6541 acggcggcgc tgctgttgcg cgaggatcgc ttcagccttg aatacgagaa gtacgccgcc
     6601 gcccggacgc gcggccccga tttcaccgtc accttcaagg gccacatgcc cttcaacgtc
     6661 gaagttcgcc gcatccgcaa cggcgaactg gacacggctg acgccggcgc gcgcacggct
     6721 aagctcatcg ccgtgctggt ggacaaggcc agccagatgc cgcccagcat catcaatgtg
     6781 ctctggctaa gtgccgaagg ggaaattgat gccgatgacc tgacccacgc cgtggcgacc
     6841 gtgcggcagt tggccgagcg caaagacgac gactacttcg cccggcgcgg ctacagggac
     6901 gcggccgact ttctggggca gtatcagcgc ctgagtggca tcgtccggcc ggccgccggc
     6961 gcgccagtac tctggcgcaa ccccacggcc cgccataaga ttccgcccga tctggccgcg
     7021 gccctcggcc gcctgagggg gccataacat gaccgaaagt cctattgcct gctttgtagg
     7081 actatgctag tatgtgcaca ccctgctgtg tacgagagtg tatctggcgt tgagccactt
     7141 agttttcttt gggaattagg atacgaaaga ggggatgtag tagccgcaag gccaggcaga
     7201 ggacctcgag acacaattcc cacctgcgtg agcaggttca cacaaaagag cattcacggc
     7261 atggcggggc gaactcatcg ctcatcccat ttgtagtgga ttaacgtttc acccatatct
     7321 atcggggtga aacgttttgt ttagggggcc ggctgataga cgacagacta ccgacggccc
     7381 gcggagcagg ctctctggcc gtcgtccccg gtctgttgcc ggtcgtccct cttggccggg
     7441 cattgcccca ctgacctact gtctacttct ccaccaacac tatatccgcc agattccgcc
     7501 cgatcgcgtg ccgcatgccg tcgatctcca ccgtcagcgg ccccaggaag ggcgcgacct
     7561 cggccaccag cacctctgct ccgggtacga ggcccagccc ggccagatag cgcagcagct
     7621 cactattggc gtcgtcgggg acgcgcgaga tgcgcgcccg cgcgccggcg ggcacgtccg
     7681 acagcgccgt cgtatcgacc acgggcattg tgccgtccag cgcggggatg ggttcgccgt
     7741 ggggatcaaa ggtggggtgg ttcagcgccg cggcgatgcg ctcctctagc ttctcgctga
     7801 tgacgtgctc caggatgtcg gcctgctcgt gaacctcgtc ccagcggaag cccagcgcct
     7861 ccatcaggta cagttccagc agccggtggt ggcgaatgac ctccaaggcc agcttctcgc
     7921 cggcggccgt cagggtgacg ccgtagtgct tctcatagtt gactaggttg agccgggcca
     7981 gacgctggat catgctcgtg gctgaggcgg gcttgaccat gcgcgcggcg gccacgcgcg
     8041 aggttgagac cgggctttct tcctgcgcca gggcgtagat agtcttcacg tagtcttcga
     8101 tggcctggtg gctaagactg tcgcgctgtt gcgacccgcc gcggtcgagt gagtcgatct
     8161 ggggcatgtc gatagcgtag acgatggggg gcgcggcgtc aagaggcgcg gccggggcta
     8221 gaagtcacgt agcagggcga cgtagccacg caggctctcg gccatttggt tgagcgagat
     8281 gctttcgccg gctgtgtggg ccatgtgctc ttcgccgggg gcgtagccga cgacggccag
     8341 gccatggggg gcgaagtggc ggccgtcggt ggcgaagcgg tagcgggtca actccggcgg
     8401 ccggcccatg cccgcggcaa tggccgctgc cgtccgcgcc accaacgggc tgtcgggcgg
     8461 cgtgtagaag ccggccaggg gcgcgctgct gccggccatt ggctccagtc cctcggcgta
     8521 ggccagcgtg tggggcgcgc cagccgccag ccggtcgatg agcgcggcca gagtatcgac
     8581 gctctccgtg gccgtgcgga agtcgagcag cacgcgcgcc caggccgggg tgacgttgcc
     8641 gctggtcgta tcgacctgga ggatggtcgg ggcgacggag gtcggcccca ggatgggatg
     8701 ggccggcagc gcggccacgg ctgcctccag cccgcccagg aaggccgcca gggcgtagtt
     8761 ggggttcagg ccgcgctccg gggcgctggc gtggacggag cggccgtgga aggtcagcca
     8821 tagctggagg atgccgcggt ggcccagcgc cagcgtattg ctcgacggct cgcccaggac
     8881 gacgagttcg accgggtagt ccaggtgctc agcccaatag atggcccccg cgccgccgac
     8941 ctcctcctcg acgacaccgc agaagacgac atcgcgccgt gggcgttggc ctgcccgccg
     9001 cagggccgcg ccggcataga cctgcaccgc cagcggcccc ttgatgtcgc acgacccccg
     9061 gccgatgaga cggtcgccgt cgatctcggc ggcgaagggg ggcaccgacc acagcgacag
     9121 gtcaccagga tcgacgtggt cgaggtgggt gttcaacacc agcgcgggca gggagcggtc
     9181 cacgccgtgc aggcggccgt agacgttgcc ggccgcgtcg ccccagacct cgtcatagcc
     9241 cagccggcgt agctcctcgg ccaccagcgc ggccaacgcc gcctcctggc cgggcatgct
     9301 gggcgtgcgg atcaggcgtt gggcgaaggc aatacactca gcgaggacag gctcccagtc
     9361 cacaggctcc tccgggttgg gcggcgcgtc aggccgcgcc gagtaggcgg acaatagccg
     9421 cggcgtcgag caccgccttg ggcacgaacg atgaggccag cacgtactcc tgatccttgc
     9481 tgccgtcgtc gctgcgctgc gaggagtggg cgttgtaccc ctccggcccc aacccgtcga
     9541 tggttggcag gctgtcccac agccagttgc cgtcgctcag gccgccgcgc gattgcaggc
     9601 tgaccgtgcc cagacccagc tcggccgccg ccgccgacca gacctcgaac agggccatcg
     9661 tgccggcgtt gggcggccag ggctgccagc gcccctcaat gtcgatggtg accttgcagg
     9721 ggtagccgtc gtaggcgctg cgcaccgtgc aggcctcgtt gagggccagc acgcggccaa
     9781 cgccctcctc gaacacgaca ggatcgtagg cgcgcatctc cacgccggcc gacgcctggt
     9841 ggggcacgcg gttgacgacc gtgccgccca taatcgtgcc gatgtt
//
LOCUS       DAOSMQ010000056         9795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6916_length_9795_cov_4.423819, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000056 DAOSMQ010000000
VERSION     DAOSMQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6916_length_9795_cov_4.423819"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PK829_03150"
     CDS             <1..767
                     /locus_tag="PK829_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU40244.1"
                     /translation="VMSRMEDMKAVAGCAAALATETGNIGYLGPLINFETRRLTASAY
                     LGARYCYENYRNLDPADLGFTVTWIGFWFNIPGVTLDPTEVVTNFFDTGTDVVLSGID
                     TTEAIDIAGQRAAQGEAVHAIPYDYIAACDIAPDVCLGVPFFSWGPPYLDIATAVSNG
                     TWQQSWDWLPANWDNLTDPALTSVGWVSGPALTGEMQANLDQFIAGMASGDINVWTGP
                     INLQDGTTYIADGATATDDEIWYLPQLLEGMDGPSE"
     gene            932..2470
                     /locus_tag="PK829_03155"
     CDS             932..2470
                     /locus_tag="PK829_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40245.1"
                     /translation="MTDLLPSGHPRIDHMEMRAITKRFPGVLASDHVDFDVRAGEVHA
                     LLGENGAGKSTLMKILYGLYQQDEGEVWLNGQVVNIQSPADAIRYGIGMIHQHFMLVP
                     TLTVKENVALGLKSSRAFLTDLDRVSARIEELARLYGLDVDPDAVVWQLSVGQQQRVE
                     IIKALYRGAALLILDEPTAVLTPQEVDEFFVILEQMKRDGHGLIFISHKLHEVIQLCN
                     RVTVLRDGRTIGTTKVEGTTKQQLAEMMVGRPFGAQPAKKPIDAHEVRLSVRDVEALN
                     DRSRPALRGVSFDVRAGEIVGLAGVSGNGQRTLAEVIAGLRPATRGEIILGGRNVTGQ
                     SPRQIMALGLSYIPEERMRDGMIREFSVAENIILREFDQPPYARSGFLNQGAIQRNSR
                     ELVNSFNVKTPSLETPAKSLSGGNIQKIILAREISRRPHVIVAAQPTRGLDIGATEYV
                     HARLLEQRDQGTATLLISEDLDEVLALADRILVICDGRIMGDVPRAEATAEKLGLLMA
                     GVES"
     gene            complement(2577..3665)
                     /locus_tag="PK829_03160"
     CDS             complement(2577..3665)
                     /locus_tag="PK829_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU40246.1"
                     /translation="MKAFVTGGTGFIGRSLVRQLVERNYQVYALARSQRGADALRAQG
                     AVVVPGDVTDRESMRAAMRGSDVVFHLAAIYDYTPEAKAQSATINVEGTRQVLGLAHE
                     LDVPRIVYTSSLAVFGDTHGELPDETYRYEGPFLTEYDRTKWQAHYEVAEPLLAEGAP
                     IIIVMPGAVYGPGDTSWLAEAMRLFYRGWLPMVPGPETTLTYAYVDDIAAGHILAAEK
                     GRVGETYILAGPAVPLGEMIEFWAQLTGKRPPVARVPARALAPLAPVAARLQPALSLS
                     PLVGEDMLHMLGATYAGRADKARAELGWRPRPLQTGMLETFEWIAATEPGNTGGRERA
                     AGMALLLAAVALLLLWLRSRLKSERAAA"
     gene            3895..5406
                     /locus_tag="PK829_03165"
     CDS             3895..5406
                     /locus_tag="PK829_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOU40247.1"
                     /translation="MDDSLDHTIDREFTRTSRLIAVTLVVLVLLEIAVFTVVFAVFGA
                     QTAGLWLPELLLAVLFSLLIGAIALNSVGRARRALSEDIGRAAAQELRALRSANNRAQ
                     SLQRMASTLSATLSFERVVEQSLDVCSLALDEMGIPRESLVGAVFLYEEGMLRPIARR
                     RFLGEDSQRVIAGKRGVVGAALSRAEPAVTNNPAEDPELRDYEAFRDCLTAVCVPLRA
                     GFQLFGAMVIGTDTPVQFEEEHFDLFKAVGDQAVIALQNAQFYQRLEAEKQRLIEADE
                     EARKELARDLHDGPTQSISAIAMRLNYARTTIDQDPARVKQELTKIEALAKKTSAEIR
                     GMLFTLRPLVLETQGLGAAIETLLRRYSESDGIEMQLVGAENGNLLSKSAQMVVFAVL
                     EEALNNARKYSQATTIEVRFWQTEGLFVAAVRDNGVGFDTYDINRDYSTRGSLGMVNM
                     RERAERIDGSLRVESSPDSGTTVTLVVPLDKQGPPAEGDERLSDQPQLIDAGS"
     gene            5360..6838
                     /gene="mptB"
                     /locus_tag="PK829_03170"
     CDS             5360..6838
                     /gene="mptB"
                     /locus_tag="PK829_03170"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HOU40248.1"
                     /translation="MSASPTSPSSSTPAADAPARGDAPWLWLAAGLAGYGLLAWWFPL
                     AANVGRTPPADIRTFAPTLPAGMLYALLVAALHGVALKAVGRATRRPLARPLATAVLG
                     GLLFALPLLFAYPVNATDVYRYLIRGRIAAVYGQSPFVAPPATFSGDPFLPLAGEWAG
                     ETSPYGPLWELVAAGVARLSGDNLLLGVVLFKLLAVACFLAAAAVLWAILRDRPGRLM
                     ATLLWAWNPALLLTFALNGHNDALMILWLLLGVWLGQRRPAAGFLVLCLAALTKPVAA
                     LALPVLFIAWWRGAAGSRARARFAAAAVLGALALTWLAFLPWAAPGRLAQTPLELIGR
                     LLREATGSGGFSPAVWLYFALGQRVAFESIGAALRALFLLLAAGVLWRGWRGRPPARG
                     AADLSFGYLTQALSFRVWYAVWPFPWLLLDADGDRAAAYRLRAGLWFLLLAQLSVVWY
                     GQWRVTVFGGDHVVAHLLGVPLVFGLPWLLARRPLPGLTHPL"
     gene            6913..8946
                     /locus_tag="PK829_03175"
     CDS             6913..8946
                     /locus_tag="PK829_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOU40249.1"
                     /translation="MAFLQEIIALLSRPPGSVIFHLLTLFALQVVLALAVSRWRRDPN
                     DEAARRMALAAGGMLAARLLLLLVALFVDQDPERARVVLPPLEQAIDTATALLLVWGL
                     AAFALDRPRLADGVLLVSLVVVAVLFLFFFQDWQSRATLTSAYIGTVQAYVWGVLQMV
                     ILAVGLVWLLVDRAARLSLRPIIVAVALLAHVANFFNYPEIIPSGTEVVYWLRLGYLV
                     AFPLWAVLAYREGMRGLVTLAEAERAQSAQVVRNLRLSTGVIAARIPEVRLEGALDMV
                     VGLAATQFAAIGLIDEKNPQRVLFNHTRPAAQRDAGYGHVFNLSDHPAFRLAYEQQRG
                     IELLPRGVGARQVHDLSQQFNVGALGPVLVEPLVVANQCFGFLFLAAPPSAVNWTDRD
                     RAIFPGVAAFLAQAIANSRRLESETPARRAPAPPPRLPEADAERERLIVELAEARRQQ
                     AVAEERARQAESMAVLLQQREPGRPTTARPQVAQSAVAPTVESTVASILPILRRKKLT
                     LDLSMAADLPLVAVKETVLRQLTLSLLENACRASAEDSGVVVRAEAVAAEDGATNGGK
                     ATSSRLVHVAFSDSGGGVAADQQGYVFDSRHHLAGGQPISGLGDTSANLAVVQKLAQA
                     SGGDLVFESQPGVGATFTLRLPAAELRPWSLLTPPARPGQTDETSRPAGAGEA"
     gene            8950..>9795
                     /locus_tag="PK829_03180"
     CDS             8950..>9795
                     /locus_tag="PK829_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU40250.1"
                     /translation="MDGQRPAGWQSNLLLAAAVGATVFLALLLAQFDALQARMLPQRS
                     GLPIVNVQATAVAVGQVIPLNPDAPPAPTAAAVPLLADDLNPRPEFITGVSESGVIFT
                     VCGEVPEGWLLYTVQPGETLASLATATAATTAELAAANCLDDGEMTAGMQILVPRQPA
                     AALCGPPQWWVRYEVQPGDTLGGLARNRGTTIDEILRANCRDSLDLLAGQLIFLPPGF
                     QGGPSAPLPQPSLTPLPTATPVPTIAPLPTVAPPTDEPPTKAPPPDTPVPPRPTDTPL
                     PPTATP"
BASE COUNT         1468 a         3326 c         3328 g         1673 t
ORIGIN      
        1 acgtcatgag ccgcatggaa gacatgaagg ccgtggccgg ttgcgccgct gccctggcga
       61 ccgagaccgg taacatcggt tacctcggcc cgctgatcaa cttcgagacc cgccgcctga
      121 cggcctcggc ctatctgggc gcgcgctact gctacgagaa ctatcgtaac ctcgacccgg
      181 ccgacctggg ctttaccgtg acctggatcg gcttctggtt caacatcccc ggcgtcacgc
      241 tggacccgac cgaagtggtg accaacttct tcgacacggg cacagacgtg gtgctgagcg
      301 gtatcgacac gacggaggcc atcgacatcg ctggccagcg cgcggcccag ggcgaggcgg
      361 ttcacgccat cccctatgac tacatcgctg cctgtgacat tgcccccgat gtttgcctgg
      421 gcgtgccctt cttcagctgg gggccgcctt acctcgacat cgccacggct gtctccaacg
      481 gcacgtggca gcagtcgtgg gattggctgc cggccaactg ggataatctg accgatccgg
      541 ccctcaccag tgtcggctgg gtcagcggcc cggccctgac cggggagatg caggccaatc
      601 tggatcagtt catcgccggc atggcctcgg gcgacattaa cgtctggact ggcccgatca
      661 acctgcagga cggcacgacc tacatcgccg acggcgctac cgcgaccgac gacgagatct
      721 ggtacctgcc gcaactgctg gaaggcatgg acggccctag cgagtaagca gtaagaagta
      781 agcagtaaac agtattcagt aggtcagcag gccagtgtgt caaaaccact gcctactgac
      841 cactgaatac tggatacggc atactgaaca ctgaatactg aatactggat actacacact
      901 ggatactgga tactggatac tggatactgc catgaccgac ctcctcccct ccggccatcc
      961 gcgcatcgac cacatggaaa tgcgcgccat caccaagcgc ttccccggcg tgttggccag
     1021 cgaccacgtc gatttcgacg tgcgcgccgg cgaagttcat gccctgctgg gcgaaaacgg
     1081 cgcaggcaag agcacgctga tgaagattct ttacggcctc tatcagcagg acgagggcga
     1141 ggtctggctg aacggtcagg tcgtgaacat ccagtcgccg gccgatgcca tccgctacgg
     1201 catcggcatg atccaccagc atttcatgct tgtgccgacg ttgactgtga aggaaaacgt
     1261 cgccctgggc ctgaagtcat cgcgcgcctt cctgaccgac ctcgaccgcg tgtccgcgcg
     1321 catcgaagaa ctggccaggc tctatggcct ggatgtcgat cccgatgccg tggtctggca
     1381 gttgtcggtg ggccagcagc agcgggtgga gatcatcaag gcgctctacc gtggcgcggc
     1441 cctgctcatc ctcgacgagc cgacggcggt gctgacgccg caggaagtgg acgagttctt
     1501 cgtcatcctg gagcagatga agcgcgacgg tcacgggctg atcttcatct cccacaagct
     1561 gcatgaggtc atccagctct gcaaccgggt gacggtactg cgcgacgggc gcaccatcgg
     1621 cacgacgaag gttgagggca cgaccaagca gcagttggcg gagatgatgg tcggccggcc
     1681 atttggcgcg cagccggcca agaagccgat tgacgcgcac gaggtgcgcc tgagcgtgcg
     1741 cgatgtggag gcgctgaacg atcgcagccg gccggcgctg cgcggcgtgt ctttcgacgt
     1801 gcgcgcgggc gagatcgtcg ggctggcggg cgtgtcgggc aacggccaac gcacgttggc
     1861 ggaagtgatt gctgggctgc ggccggccac gcggggcgag atcatcctcg gcggccggaa
     1921 cgtcaccggc cagtcgccgc gccagatcat ggctctgggc ctgtcgtaca tccccgaaga
     1981 gcgcatgcgc gacggcatga tccgcgagtt cagcgtggcc gagaacatca tcctgcgcga
     2041 attcgaccaa ccgccctacg cccgctccgg cttcctcaac cagggagcca ttcagcgcaa
     2101 ctcgcgcgag ctggtcaata gcttcaatgt caagacgccc tcgctggaga cgccggccaa
     2161 aagcctctcg ggcggcaaca tccagaagat tatcctggcc cgcgagatct cgcgccggcc
     2221 gcatgtcatc gttgccgccc agcccacgcg cggcctcgac atcggcgcga ccgaatatgt
     2281 ccacgcccgg ctgctggagc agcgcgacca gggcacggcc acgctgctca tcagcgaaga
     2341 cctggacgaa gttctggccc tggcggaccg cattctggtc atctgcgacg gccgcatcat
     2401 gggcgacgtg ccccgcgccg aggcgacggc ggagaagttg gggttgctga tggcgggggt
     2461 ggagagctag gcgcgccggt gcgtcgcact ttctgaagtg cgtcgcacct tcttcagggc
     2521 ggagcgccag cgcacacctg cacgttgggg cagacctgtg cgccggccct cgactactaa
     2581 gccgccgccc gctcggactt caagcgagag cggagccaca gcaatagtag cgcgacggcg
     2641 gccagcagca gggccatccc cgcggcgcgt tcgcgcccgc cggtgttgcc cggctccgtc
     2701 gccgcgatcc actcgaatgt ctccagcatg cccgtttgca gcggccgggg ccgccaaccc
     2761 aactcggcgc gagccttgtc ggcccgcccg gcataggtcg cgcccagcat gtgcagcatg
     2821 tcttcgccca ccagcggcga tagcgacagc gctggctgga gacgggcggc caccggagcc
     2881 aacggtgcca gggcgcgggc gggcacacgg gccacgggcg gccgcttgcc ggtcagttgc
     2941 gcccagaact cgatcatctc gcccaggggc acggccgggc cagccaggat gtaggtctcg
     3001 ccgacgcgcc ccttctcggc ggccaggatg tggcccgcgg cgatgtcgtc tacgtaggcg
     3061 taggtcaacg tcgtttccgg gccgggcacc attggcagcc agccacgata gaacaggcgc
     3121 atggcctcgg ccagccagct cgtgtcgccg gggccataga ccgccccggg catgacgatg
     3181 atgatcggcg cgccctcggc cagcagtggt tcggcgactt cgtagtgggc ctgccacttg
     3241 gtgcggtcgt actcggtcag gaacggcccc tcatagcgat aggtctcgtc gggcagctcg
     3301 ccgtgggtgt cgccgaagac ggccaggctg ctggtgtaga cgatgcgcgg cacgtccaac
     3361 tcgtgggcca gccccaatac ctggcgtgta ccttcgacgt tgatggtcgc cgactgggct
     3421 ttggcctctg gcgtgtagtc gtagatggcg gccaggtgga aaacgacgtc gctgccgcgc
     3481 atggccgcgc gcatcgactc gcggtcggtc acgtcgccgg gaacgacgac cgccccctgg
     3541 gcgcgcagcg cgtcggcccc gcgctgcgaa cgcgccagag catagacctg atagttgcgc
     3601 tccaccagtt ggcgaacaag actgcgaccg atgaacccgg tgccaccagt gacaaatgct
     3661 ttcatgggtg tactccctgc gatggtcgcg gcgcttggac ggcgcgcccg aacgactatc
     3721 gtcccattat agctgtggcg gcgcggattg gtgaacaatc ggggcgatct ggtatgattg
     3781 acaagtggaa tcgccgtagc gtcatggcca atgtaaggta gaaacgttac actgtagacg
     3841 gtaaattgga ctaggacgcg ccgccgcgcc gccggccaga cgtccgtata cagaatggat
     3901 gactcgctag accacaccat cgaccgcgaa ttcacgcgca cgagccggct catcgccgtc
     3961 accctggttg tcctggtgct gctggagatt gccgtcttca ccgtcgtttt cgccgttttc
     4021 ggcgcgcaaa cggccggcct gtggttgccg gagttgttgc tggccgtgtt gtttagcctg
     4081 ctgatcggcg ctattgccct gaacagcgtc ggccgtgctc ggcgcgcgtt gtccgaagac
     4141 attggccggg ccgccgccca ggaactgcgc gccctgcgct cggccaacaa tcgcgcccaa
     4201 tcgctgcaac gcatggcctc gaccctcagc gccacgctca gctttgagcg cgtcgtggag
     4261 cagtcgctcg atgtctgtag cctggcgctg gacgagatgg gcatcccccg cgagtcgctg
     4321 gtcggcgcgg tctttctcta tgaggaaggc atgctgcggc ccattgcccg gcggcgcttc
     4381 ctgggcgagg acagccagcg cgtcatcgcc ggcaagcgtg gcgtcgttgg cgcagccctc
     4441 agccgggccg agccggccgt caccaacaac ccggccgaag accccgaatt acgcgactat
     4501 gaggccttcc gcgattgtct gaccgctgtc tgcgtgccct tgcgagccgg tttccagctg
     4561 ttcggcgcga tggtcattgg caccgatacc cccgtgcagt ttgaagagga gcattttgac
     4621 ctcttcaagg ccgttggtga ccaggccgtc atcgccttgc agaacgctca gttctatcaa
     4681 cgcctggagg cggaaaagca gcggctgatc gaggccgacg aggaggcgcg caaggagttg
     4741 gcccgcgacc tgcacgacgg cccgacacag agtatctccg ccatcgccat gcgcctcaac
     4801 tatgcccgca caaccatcga ccaggacccg gcacgggtga agcaggaact gaccaagatc
     4861 gaggcgctgg ccaagaagac ctcggccgag attcgcggca tgctcttcac gctgcgtccg
     4921 ctggtgctgg agacacaagg gctgggcgcg gccatagaga cgctgttgcg tcgctacagc
     4981 gagagcgatg gcatcgagat gcagctcgtt ggcgcggaga acggcaattt gctgtccaag
     5041 tcggctcaga tggtcgtttt tgccgtcctg gaggaggcgt tgaacaacgc ccgcaagtac
     5101 tcccaggcca cgactatcga ggtgcgcttc tggcaaaccg aagggctatt tgtagccgcc
     5161 gtgcgcgaca acggcgttgg cttcgacacc tacgacatca accgcgacta cagtacccgc
     5221 ggcagcctgg gcatggtcaa catgcgcgag cgggccgagc gcatcgacgg ctcgctgcgc
     5281 gtcgagtcgt cgcccgactc tggcacgaca gtgacgctgg tggtgcccct ggacaagcaa
     5341 ggcccgccgg ccgagggcga tgagcgcctc tccgaccagc cccagctcat cgacgccggc
     5401 agctgacgcg ccggcgcggg gcgacgcgcc ctggctctgg ttggcggccg ggctggccgg
     5461 ctatgggctg ttggcgtggt ggttccctct ggcggccaac gtcggccgca cgcccccggc
     5521 cgacatccgc acctttgccc cgacactgcc ggcggggatg ctctacgcgc tgctggtggc
     5581 ggcgctgcat ggcgtcgccc tgaaggctgt agggcgggcc acccgtcggc cgctggcccg
     5641 gccgctggcc acggccgtgc tgggtgggct gctgttcgcc ctgccgctgt tgttcgccta
     5701 ccccgtcaac gccactgatg tctaccgcta cctcatccgc gggcgcatcg ccgccgtcta
     5761 tggtcaaagc ccgttcgtcg cgccgccggc gacgttcagc ggcgatccgt ttctgccgct
     5821 ggcgggcgag tgggccgggg agacgtcgcc ctatgggccg ctgtgggaac tggtggccgc
     5881 gggcgttgcc cggctgagcg gcgacaacct gctgctgggc gtggtgctgt tcaagctgct
     5941 ggccgtggcc tgcttcctgg cggcggcggc ggtgctgtgg gccattctgc gcgaccggcc
     6001 cggacggctc atggcgacgc tgctatgggc ctggaacccg gccttgctac tgactttcgc
     6061 cctcaacggc cacaacgacg ccctgatgat cctctggctg ctgctgggcg tctggctggg
     6121 gcagcggcgg ccggcggctg gcttcctggt gctgtgcctg gccgcgctga ccaagccagt
     6181 ggctgcgctg gcgctgccgg tcctcttcat cgcctggtgg cgcggggcgg ctggcagccg
     6241 ggcgcgggcg cgcttcgcgg cggcagccgt gctgggagcg ctggcgctga cgtggctggc
     6301 cttcctgccc tgggccgcgc ccggccgcct ggcccagacg ccgctggaac tcatcggccg
     6361 gctgctgcgc gaggccaccg gcagcggcgg cttctcgccg gcggtgtggc tctactttgc
     6421 cctggggcag cgcgtggcct tcgagagcat cggcgcggcg ttgcgggcgt tgttcctgct
     6481 gctggccgcc ggtgtgctgt ggcgcggctg gcgcggccgg ccgccggcgc ggggcgcggc
     6541 cgacctgtcg ttcggctacc tgacccaggc gctcagcttc cgggtgtggt acgccgtctg
     6601 gccgttcccc tggctgctgc tagacgcgga tggcgaccgc gccgctgcct accgattgcg
     6661 cgccgggctg tggttcctgc tgctggccca gctgtcggtc gtctggtatg ggcagtggcg
     6721 cgtgacggtt ttcggcggcg accatgttgt tgcccacctg ctgggcgtgc cgctggtgtt
     6781 cgggctgccg tggctactgg cgcgccgacc gctgcctggc ttaacgcatc ctttatagtc
     6841 agcgcccggc ctttctgcta tcctagccct gagagtggcg cgccccagcg cgaggcgcga
     6901 ctgttctaat ctatggcgtt cctacaagag atcatcgccc tgctgagccg gccgccgggc
     6961 agcgtcattt tccacctact gaccctcttc gcgttgcagg tggtgttggc gctggctgtc
     7021 tcgcgctggc gacgcgaccc caacgatgag gctgcccgcc gcatggcgtt ggccgccggg
     7081 ggaatgttgg ccgcgcggct gctgctgttg ctggtggccc tcttcgtcga ccaggacccc
     7141 gagcgcgccc gcgtcgtcct gccgccgctg gaacaggcca tcgacacggc cacggcgctg
     7201 ctgctggtct gggggctggc cgccttcgct ttggatcggc cgcgcctggc cgatggcgtg
     7261 ttgctggtca gtctggtggt tgtggccgtg ctctttctct ttttcttcca ggactggcag
     7321 agccgggcga cgctgaccag cgcctacatc ggtacggtgc aggcctacgt ctggggcgtg
     7381 ctgcaaatgg tcattctggc cgtggggctg gtgtggctgc tcgtcgaccg ggcagcgcga
     7441 ctgtcgttgc ggccgatcat cgtcgccgtg gccctgctgg cccacgtggc taacttcttc
     7501 aactacccgg agatcatccc gtcgggcacg gaggtggtct actggctgcg gctgggctat
     7561 ctggtcgctt ttcccctgtg ggccgtgctg gcctatcggg agggcatgcg cggcctggtg
     7621 acgctggccg aggctgagcg cgcccagtcg gcccaggtag tgcgcaacct gcgcctgtct
     7681 accggcgtca tcgccgcgcg catccccgaa gtgcgcctgg agggggcgct ggacatggtc
     7741 gtcgggctgg cggcgacgca attcgccgcc atcggcctga tcgacgagaa gaacccgcag
     7801 cgtgtgttgt tcaaccacac ccggccggct gcccagcgcg atgccggcta tggccacgtc
     7861 ttcaacctgt ccgaccatcc cgccttccgg ctggcctatg agcagcagcg cggcatcgag
     7921 ttgttgccgc ggggcgtcgg cgcgcggcag gtgcatgacc tgtcgcagca gttcaacgtc
     7981 ggcgcgctgg ggccggtgct ggtagagccg ctcgtcgtcg ccaaccagtg cttcggcttt
     8041 ctgttcctgg ctgcgccgcc gagcgcggtc aactggaccg accgcgaccg ggccatcttc
     8101 cccggcgtgg ccgcttttct ggcccaggcc atcgccaaca gccgccgcct ggaaagcgag
     8161 accccggccc ggcgcgcgcc cgcgccgcct ccccgtctgc cggaggccga cgccgagcgc
     8221 gagcggctga tcgtcgagtt ggccgaggcc cgccggcagc aggccgtggc cgaggagcgc
     8281 gcccgccagg ctgagtcgat ggccgtgctg ttgcaacagc gcgagccagg ccggccgacg
     8341 accgcccgac cccaggtggc ccagtcggcc gtcgctccaa ccgtcgagag cacggttgcc
     8401 tccatcctgc ctattctgcg acgcaagaag ctgacgctcg acctgtctat ggccgccgat
     8461 ctgccgctgg tggcggtcaa agagacggtc ttgcgccagc tgacgttgag cttgctagaa
     8521 aatgcctgcc gggcgtcggc cgaggacagt ggcgtcgtcg tccgcgccga ggccgttgcc
     8581 gccgaggacg gcgcgaccaa cggcggcaag gcgaccagca gccgcctggt ccacgtggcc
     8641 tttagcgatt cgggcggcgg cgtggccgcc gaccagcagg gctacgtctt cgactcgcgc
     8701 caccacctgg ccggcgggca gccgatttcc ggtctgggcg acactagcgc caatctggcc
     8761 gtcgtgcaga aactggccca ggccagcggc ggcgacctgg tcttcgaaag ccagccgggc
     8821 gtgggcgcga ccttcacctt gcggctgccg gcggccgagc tgcggccgtg gtcgctgctg
     8881 acgccgcccg ccaggccagg ccagaccgac gaaacgtcca ggccggccgg ggcgggggag
     8941 gcctagccga tggacggcca acgaccggcc ggctggcaat cgaacctcct cctggcggcc
     9001 gccgtggggg cgacggtctt cctggcgctc ctgctggccc agttcgacgc cctacaggcg
     9061 cgcatgttgc cgcagcggtc gggcctgccc attgtcaacg tccaggccac ggccgtggcc
     9121 gttggccagg tcattcccct gaaccccgac gcgccgccag cgccgacggc cgcggccgtg
     9181 ccgctgctgg ccgatgacct caacccgcgg ccggagttca tcaccggtgt cagcgaatcg
     9241 ggggtcatct tcaccgtctg cggcgaagtg ccggagggtt ggttgctcta cactgtccag
     9301 ccgggcgaga cgttggcctc gttggccacg gcaacggcgg cgacaacggc cgagttggcc
     9361 gcggccaact gcctggacga cggcgaaatg accgccggaa tgcaaatact cgtgccccgg
     9421 cagccggcgg cggcgctgtg tgggccgccg caatggtggg tgcgctacga ggtgcagccg
     9481 ggcgatacgc tcggcgggct ggcccgcaac cggggcacga ccatcgacga aatcttgcgc
     9541 gccaactgcc gcgattcgct cgatctactg gccgggcagc ttatcttctt gccgccgggc
     9601 ttccagggcg ggccgtcggc gccgctgccc cagccgtcgc tgacgccgct gccgacggcg
     9661 acgcccgtgc cgacgatcgc gccgttgcca acagtggctc cgcccaccga cgagccgccc
     9721 accaaagcgc caccgccgga tacgcctgtg ccgccacggc ccaccgacac gccgctgccg
     9781 ccgaccgcta cgccg
//
LOCUS       DAOSMQ010000057         9775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_6940_length_9775_cov_4.099486, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000057 DAOSMQ010000000
VERSION     DAOSMQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9775
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6940_length_9775_cov_4.099486"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK829_03185"
     CDS             complement(<1..346)
                     /locus_tag="PK829_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40251.1"
                     /translation="MLIVSRRRFLLPGVGLSLLFALALALLRGAPVFAAGDRYVATTG
                     VDAGACDNSASPCQTIAYAVAQAAAGDTIHIAAGSYTGVGNQHVVIAKALTLAGADAA
                     TTILTYDPLVQAV"
     gene            complement(510..2051)
                     /locus_tag="PK829_03190"
     CDS             complement(510..2051)
                     /locus_tag="PK829_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40252.1"
                     /translation="MLRRLVLLATTLVLLLTLGLWRSSAAAPDAGWWDARWPYRVAVD
                     VDAGGFARNDKVADVGINFTDLLNAVGQAEKFDPDSIRVLEVDGNAIVDEAVPFQFDR
                     ASDFNPNTNAAGTLVILLTGATAADETRHYQVYFDIIGGGYDVPVFQERVTVGNLTDA
                     YGFTAFRIDNDHGRYIYHKTGGGFSALIDASGKDWISWNTNPKAAGDYRGIPNMVHPN
                     DGGYFHPGRANVNSTLTRRGPLKATIRSTSTDGLWTTQWEFYPDAARLTVIKIAAGKA
                     YYLLYEGTPGGKLDLTTDLVVRSDGTTTPASQSWAADLVGEEWVYFADPGLGRSLYAY
                     HNVDDDKIDSYAPDGADMTIFGFGRNGNSRLLTEAGQQMTIGLVDETALAGVSAAMYA
                     AVRPLGVTLGQTQERPATPTPTPSPTLRPTDTPTPTATASPTPTATATPTQTLTPSPT
                     ATATATATHTPTATATNTPTATASPTPTATASPTATLKPTRGPSPTPTQKPDEDAFLP
                     FIIGD"
     gene            complement(2133..4097)
                     /locus_tag="PK829_03195"
     CDS             complement(2133..4097)
                     /locus_tag="PK829_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40253.1"
                     /translation="MVSKSRSMVAARRVLEGVVLAFLLLVGLGRASRRLVLAAGEPQY
                     EHIVTVNGNGFARTNKVVEVALNFTPLLASQGGSGALDPASIRVYEINAGGDVIDADV
                     PFQFDRAGDYNATNKARGTLVFLMTGSTGANETRRYNVRFDVTGTGFNAPSFTDRVTL
                     TDGVAHKGYQSVRVVTADAEYFYHKPGGGFATLLDANNNDWINWNTANGGAGDFRGIP
                     NMVHPNDGGYFHPGRNSVSTTVVYDGPLRATFRSTSNNGAWQVQWDVFPEYARMTVLK
                     TPGTNYWFLYEGVPGGQLQLGSDRMTRSNGSSIPASGTWDTDIPGDEWVFASDPGVGR
                     SLFLAHHQSDTKIDGYYADGSNMTVFGFGRSNNQRMLNGLPRQFTIGFVDATAIGGVE
                     PVVNAAYKPLTITGANDDGGDPGPTCDPQSYDVYFSPKANKTVGGVVLADEDIVRYDG
                     STCEVSVVFDGSAHGLAASANIDAFALQGGDTYFSLLAAGNVPGLGKVDDSDVIKYNG
                     SAFTLFFDGSAHGLTVDAEDVDAIAFDETGKLLLSTSGSYAVPGLAKGGDEDLLRYDA
                     GTWELRFDGSHNAGLASEDIVGASVAANGDIYLSVLDAFNVGGISGNANDIFICTPSS
                     LGDHNTSCTYSLFWDASTFGLPPFDAFDIQ"
     gene            complement(4270..>5533)
                     /locus_tag="PK829_03200"
     CDS             complement(4270..>5533)
                     /locus_tag="PK829_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40254.1"
                     /translation="TNGTVVATIGAGVAQDAASNPNAASTSTDNTVTYTGIDTTAPTV
                     TINQASGQADPTSTSPINFTVVFSEPVTGFATGDVTLSGTAGATTAVVTQIAPNDGTT
                     YNVAVSGMTTNGTVVATIGAGVAQDAAGNPNAASTSTDNTVTYNAPAATASIYVSTTA
                     AGSVGAVSFGSEDILLWDGNAWSVWFDGSAAGLQGTKNNINAFYIPEVGGAANSDTVL
                     MSFVQNARVVPGIPGKVDGMDLVTWNGSAFSLTFDGQDVGLTVLTAEKIDGLHELDPS
                     LAPAAVKAAAGGTCLRYFLISTAGNGKVMNYDGTTLKISGEDVLGFCATHLGTNTTGK
                     WHMLLDGSAEGVGPNNITSIAASADGQTLFLTTNKAFNVDSAVGGHSMVYEYDMTSHT
                     FSGPIFSAPAAGLEQKVDGLHMVDNNIN"
     assembly_gap    5534..5633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5634..7996)
                     /locus_tag="PK829_03205"
     CDS             complement(<5634..7996)
                     /locus_tag="PK829_03205"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HOU40255.1"
                     /translation="MNVCGGDVAPTVTSTTPANSATNVALAADISVTFSEAVTVSGAW
                     YTISCANSGTHTATVSGGPTTFTLNPDINFTGGELCTVTIESTLVADQDGTADNMTAD
                     YTFSFTTTTPPAVCGDPATPIHDVQGNGAASPDDGVVRTIEGIVVGDFQDYTTDPPNE
                     ISGYFLQEEDAQVDADPNTSEGIFVFDGQNPATNVALGDKVRVVGTVDEFNNLTELTS
                     VSNTQVCSSGNTLPTAASITLPVTALTDYEKFEGMRVTFPQELTVTDTFTLGRFGEIL
                     LSEGGRLPSPTDAAEPGAPAQAVRAANILRSIFLDDGLTNQNPDPLIHPAPGLSASNT
                     LRGGDTTTGLTGVLYYLGTTSDYRIMPSEAITWNHENPRPTDAPVISGTLQIVGSNTL
                     NYFITLDNPNTTADNICGPANNQECRGANTTQEFTRQRDKFLNALAEMDADVFGLMEL
                     ENNSAANPAGDDNDPVLEDIVNGLNDLVGAGTYDFIDTGTLGGDAIKVGLIYKVATVT
                     PVGDYAVLDSSVDPLFDTSRNRPALAQTFEEIATGGQLTVVVNHFKSKGSGCGAGDDV
                     ADTSGGNCNGTRTDAATALVNWLATDPTGSGDPDFLVIGDLNSYAKEDPIDVFINAGY
                     VDLADEYNTDPYSYSFDGEWGYLDYALANPSLATQVTDAKEWHVNADEPPILDYNTEF
                     KSPGQISSFYSPDPFRWSDHDPIIVGFDLDVPDTTAPTVTINQASGQTDPTSTSPINF
                     TVVFSEPVTGFATGDVTLSGTAGATTAVVTQIAPNDGTTYNVAVSGMT"
     gene            8963..9589
                     /locus_tag="PK829_03210"
     CDS             8963..9589
                     /locus_tag="PK829_03210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU40256.1"
                     /translation="MNDQITDHLRRAYDAKAEARNAGTQSSWKLVERAAFLEQLRAAG
                     ARRLLEIGGGPGHSSAFFRDAGLDVVMTDLSPEMVRLARAKGLDARVMDFRRLDFPDA
                     SFDAVFALNCLLHVPSAELPRALAEVRRVLQPNGLFYYGVYGGYSFEGVFADDAYEPK
                     RYFVFYPDDELRRRAAAAGFDEVSFRTIPLEGDDLHFQELVLRPEERV"
     gene            9589..>9775
                     /locus_tag="PK829_03215"
     CDS             9589..>9775
                     /locus_tag="PK829_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU40257.1"
                     /translation="MVVIGDIHGQYVKMLTVLHDARLIDAAGHWSGGATTLWFMGDYF
                     DRGPDGIAAIDLIRALQD"
BASE COUNT         1606 a         2908 c         3328 g         1833 t
ORIGIN      
        1 cgacggcctg caccaatggg tcataggtca gaatcgtcgt cgccgcgtcg gctccagcca
       61 acgtcaacgc cttagcgatg acgacgtgct ggttaccaac gccggtgtag ctgccggcgg
      121 cgatgtggat agtgtcgccg gccgcggcct gagccacggc gtaggcgatg gtctggcagg
      181 gcgaggcgct gttgtcgcac gcgccggcgt cgacgccggt cgtggcaaca tagcgatccc
      241 ccgctgcgaa tacgggcgcg ccccgcagga gcgccaatgc cagggcaaat agcaaggaaa
      301 ggcccacccc tggcagcagg aaccgacgcc tgctgactat caacatacca atacctcccg
      361 actataattc cgatacagtt ctttctatag agtcgggctg ctcacggcga agaggcagcc
      421 cgagcaggct gcatgcgcgc atccttgcct atcattgttg ccactgaggc ttacagaccg
      481 tcttacgctg tcccgttgct ccagcctggt caatcgccga tgatgaaagg cagaaacgcg
      541 tcttcgtcgg gcttctgcgt gggcgtgggt gaggggccgc gcgtgggttt cagggtggcc
      601 gtggggctgg cggtggccgt gggcgttggc gatgccgtcg ctgtcggcgt gttcgtcgct
      661 gtggctgtgg gggtgtgggt ggccgtcgcc gtggctgtgg ccgtgggcga gggcgtcagg
      721 gtctgcgtcg gcgtggccgt ggccgtcggc gttggcgacg ccgtcgctgt cggtgtgggc
      781 gtatcggtcg gccggagcgt cggcgagggg gtgggcgtcg gcgtggccgg ccgttcctgc
      841 gtctggccca gagtcacgcc cagcgggcgg acggcggcgt acatggctgc ggacacgccg
      901 gcaagggcgg tctcatcgac cagcccgata gtcatctgct gccccgcctc ggtcaggagg
      961 cgcgagttgc cgttgcggcc aaagccgaag atggtcatgt ccgcgccgtc gggggcgtag
     1021 gaatcgatct tgtcgtcgtc cacattgtgg taagcataga gcgagcgccc cagacctggg
     1081 tcagcgaagt aaacccactc ctcgcccacc agatcggcgg cccagctctg gctggccggg
     1141 gtggtcgtgc catcggagcg gacgaccagg tcggtcgtca ggtcgagttt gccgccgggc
     1201 gtgccctcat agagcagata gtaggccttg cccgcggcga tcttgatcac cgtcagccgc
     1261 gccgcatcgg ggtagaactc ccactgcgtc gtccagaggc cgtcggtcga ggtggagcgg
     1321 atggttgcct tcaacgggcc gcgccgcgtc agggtgctat tgacgttggc gcggccgggg
     1381 tggaagtagc cgccatcgtt ggggtgcacc atgttgggga tgccgcggta gtcgccggcc
     1441 gctttgggat tggtgttcca actaatccag tccttaccac tggcgtcgat gagcgccgaa
     1501 aagccgccgc ccgtcttgtg gtagatgtag cggccgtggt cgttgtcgat gcgaaaggcg
     1561 gtgaagccgt aggcgtcggt caggttgccg accgtcacac gctcctgaaa gacgggcacg
     1621 tcgtagccgc cgccgatgat gtcgaagtaa acctggtagt ggcgggtctc gtcggccgcc
     1681 gtcgccccgg tcagcaggat aaccagcgtg cccgcggcgt tggtgttggg gttgaagtcg
     1741 ctggcacggt cgaactggaa cggcacagcc tcatcgacga tggcattacc gtccacctcc
     1801 agaacgcgga tcgagtcggg atcgaacttc tccgcctggc ccacagcgtt cagcaggtcg
     1861 gtaaagttga tgcccacatc ggccaccttg tcattgcggg caaagccgcc agcgtccaca
     1921 tcgacggcca cgcgataggg ccagcgcgca tcccaccaac cggcatccgg cgcggcggcg
     1981 ctggagcgcc acaggcccag cgtgagcaaa aggacaagag tcgtggccaa gagtaccaat
     2041 cgtcgcagca tagcatctcc ccaccaccgc gcgcggcggg ccgggtgtgg cccgccgcgc
     2101 gcggcaacaa agttcagccg gaggggctga tcttactgga tatcgaacgc atcgaacggc
     2161 ggcaagccga acgtactcgc gtcccagaac aggctgtagg tgcaggatgt gttgtggtcg
     2221 cccagactgc tgggcgtgca aatgaagatg tcgttggcgt tgccggagat gccgcccaca
     2281 ttaaaggcat cgagcacgct caggtaaatg tcgccgttgg ccgctaccga cgcgccgacg
     2341 atgtcttcgc tcgccagccc ggcgttgtgg gagccatcga agcgtaactc ccacgtcccg
     2401 gcgtcatagc gcaacaggtc ttcgtcgccg cccttggcca ggccgggcac ggcataagaa
     2461 cccgacgtgc tcagcagcag tttgcccgtc tcgtcaaagg cgatggcgtc cacatcttcg
     2521 gcatcgaccg tcaggccgtg ggccgagccg tcaaagaaga gcgtgaaggc cgagccgttg
     2581 tacttgatca cgtcggagtc atcgaccttg cccaggccag gcacattgcc cgcggccagc
     2641 aggctgaaat acgtatcgcc gccctgcagg gcaaaggcgt cgatgttagc cgacgcagcc
     2701 aggccgtggg ccgagccgtc gaagaccacg ctcacctcgc acgtcgagcc gtcgtagcga
     2761 acgatatcct cgtcggccag gacaacgccg cccacggtct tgttggcctt ggggctaaag
     2821 tagacgtcgt aggactgcgg gtcacaggtt gggccgggat cgccgccgtc gtcattcgct
     2881 ccggtgatag tcagcggctt gtaggccgcg ttcactaccg gttcgacgcc gccaatggct
     2941 gtcgcgtcaa caaagccgat ggtgaactgc cgtggcaggc cgttgagcat gcgctggttg
     3001 ttgctgcggc cgaagccgaa gacggtcatg ttgctgccgt cggcgtagta gccgtcgatc
     3061 ttggtgtcgc tctggtgatg cgccaggaag agcgagcggc cgacgccagg atcgctggca
     3121 aagacccact catcgccggg gatatcggta tcccacgtgc cggaggcggg aatgctgctg
     3181 ccgttggagc gggtcatacg gtcgctgccg agttggagct ggccgccggg cacgccctca
     3241 taaaggaacc agtagttggt tcccggcgtc ttcagcacgg tcatgcgtgc gtattccggg
     3301 aacacgtccc actgtacctg ccacgcgccg ttgttgctgg tcgaacggaa ggtggcccgc
     3361 agcggcccgt cgtagaccac tgtggtgctg acgctgttgc ggccggggtg gaagtagccg
     3421 ccatcgttgg ggtgcaccat gttgggaatg ccacggaagt cgcccgcgcc gccattggcc
     3481 gtattccagt tgatccagtc gttgttgttg gcgtcgagca gcgtggcaaa gccgccgccg
     3541 ggcttgtgat agaagtactc agcatcggct gtgacgaccc gcacgctctg ataacccttg
     3601 tgggccacgc cgtcggtcag ggtcacgcga tcggtaaagg acggcgcgtt gaagcccgtg
     3661 cctgtcacat cgaagcgaac gttatagcgg cgggtctcgt tcgcgccggt gctgcccgtc
     3721 atcagaaaaa ccagcgtgcc gcgagccttg ttggtggcat tatagtcgcc cgcccggtcg
     3781 aactggaagg gcacgtcggc gtcgatgacg tcgccgccgg cattgatctc atagacgcgg
     3841 atggaggccg gatcgagcgc gccggagcca ccctggctgg ccagcagagg cgtgaagttc
     3901 agcgctacct caacgacctt attcgtgcgg gcaaagccgt tgccgttcac ggtgacaata
     3961 tgctcatatt gcggctcgcc agccgccagg accagccgcc gcgaagcgcg accaagcccc
     4021 accagtaata ggaatgccag caccactcct tccaggacgc ggcgcgcggc gaccatcgat
     4081 ctgctcttgg aaaccatggg aatacctctt ccaggaataa gcatgcgctg aagatatcat
     4141 gctccaaccg atccattata aacaaatgcc taagcgggag cttaaggacc ggtaacaagg
     4201 cggtaacaaa actgggcggc gtctggcgac gccgcccagt tcattcagtt agataagggc
     4261 aacgtggccc tagttgatgt tgttatccac catgtgcagg ccgtccacct tctgctccag
     4321 ccccgcggcc ggcgccgaga agatcggccc actgaaagta tggctggtca tgtcgtactc
     4381 gtagaccatt gagtgcccac cgaccgcgct gtcgacgttg aacgctttat tggtggtcag
     4441 gaaaagcgtc tggccgtcgg cgctggcggc gatgctggtg atgttgttcg ggccaacgcc
     4501 ctcggccgag ccgtcgagca acatgtgcca cttgccggtc gtgttcgtcc ccaggtgcgt
     4561 ggcgcagaag cccagcacat cttcgccgct gatcttcagc gtcgtgccgt cgtagttcat
     4621 caccttgccg ttgccggcgg tgctgatcag gaagtagcgc aggcacgtgc cgccggccgc
     4681 ggccttgacc gctgccggag ccagcgacgg gtccagctca tgcaggccgt cgatcttctc
     4741 cgccgtcagg acggtcagac cgacgtcctg gccatcgaag gtcagcgaga aggcgctgcc
     4801 gttccaggtg accagatcca tgccatccac cttgccgggg atgccgggga cgacgcgcgc
     4861 gttctggaca aaggacatta gcacggtgtc gctgttggcc gcgccgccga cttcggggat
     4921 gtagaaggca ttaatgttgt tcttagtgcc ttgcagaccg gcggccgagc cgtcgaacca
     4981 gaccgaccag gcgttgccgt cccacagcag gatgtcctcg ctgccgaacg acactgcgcc
     5041 gacagaaccg gcggctgtcg tgctgacgta gatgctggct gtggccgcgg gggcattgta
     5101 ggtcacggtg ttatcggtcg aggtcgaggc cgcgttcggg ttgccggccg cgtcctgggc
     5161 cacgcccgcg ccgatggtgg cgacgaccgt gccattggtg gtcatgccgc taacagccac
     5221 gttgtaggtc gtgccgtcgt tgggcgcgat ctgggtcacg acggccgtcg ttgccccggc
     5281 cgtgccgctc aaggtcacgt cgccggtggc aaagccggtg accggctcgc tgaagacgac
     5341 ggtgaagttg atcgggctgg tcgaggtcgg gtcggcctgg ccgctggcct ggttgatggt
     5401 caccgtcggc gcggtcgtgt cgatgccggt gtaggtcacg gtgttatcgg tcgaggtcga
     5461 ggccgcgttc gggttgctgg ccgcgtcctg ggccacgccc gcgccgatgg tggcgacgac
     5521 cgtgccattg gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcatgc
     5641 cgctgacggc gacgttgtag gtcgtgccgt cattgggcgc gatctgggtc acgacggccg
     5701 tcgtcgcccc ggccgtgccg ctcaaggtca cgtcgccggt ggcgaaaccg gtgaccggct
     5761 cgctgaagac aacggtgaag ttgatcgggc tggtcgaggt cgggtcggtc tggccgctgg
     5821 cctggttgat ggtcaccgtc ggcgcagtcg tgtcgggcac gtccaggtcg aagccgacga
     5881 tgatcggatc gtggtcagac cagcggaagg gatcggggct gtagaagctg ctgatctgcc
     5941 cgggcgactt gaactcggtg ttgtagtcga ggatgggcgg ctcgtcggcg ttgacgtgcc
     6001 actccttggc gtcggtcacc tgggtagcca gcgacgggtt ggccagggcg tagtcgaggt
     6061 agccccactc accgtcaaag ctgtaggagt agggatcggt attgtactca tcggccaggt
     6121 caacataacc ggcgttgatg aagacgtcga tcgggtcttc cttggcgtag ctattcaggt
     6181 cgccgatgac caggaagtcg gggtcgccgc tgcccgtcgg atcggtggcc agccagttga
     6241 ccagggccgt ggccgcgtcg gtgcgcgtgc cattgcaatt gccacccgac gtatcagcca
     6301 cgtcgtcgcc cgcgccgcaa cccgagcctt tcgacttgaa gtggttgacc acgacggtca
     6361 gttgcccgcc cgtggcgatc tcctcgaagg tctgggccag ggccggccgg ttgcgggagg
     6421 tgtcgaacag gggatcgacg ctcgaatcga gcacggcgta gtcgccgacg ggcgtcactg
     6481 tggccacctt gtagatcagg ccgaccttga tggcgtcgcc gcccagcgtg ccggtgtcga
     6541 tgaagtcgta cgtgcccgcg ccaacaagat cgttcaggcc gttgacgatg tcttccagta
     6601 cggggtcgtt gtcgtcgcca gccgggttgg cggcggagtt gttttccaac tccatcaggc
     6661 cgaagacatc ggcgtccatc tcggccaggg cgttgaggaa cttgtcgcgc tgacgggtga
     6721 actcctgggt tgtgttcgcg ccgcggcact cctgattgtt ggccgggccg caaatgttat
     6781 cggccgtcgt gttgggattg tcgagcgtaa tgaagtagtt gagcgtgttg gagccgacga
     6841 tctggagcgt gccgctgatg accggcgcgt cggtcggccg cgggttctcg tggttccagg
     6901 tgatggcctc gctgggcatg atgcggtagt cgctggttgt gcccaggtaa tagaggacgc
     6961 cggtcaggcc cgtcgtcgtg tcgccgccgc gcagcgtgtt gctggcgctc aggccggggg
     7021 cggggtggat gagcgggtcg gggttctggt tggtcaggcc gtcatccagg aagatgctgc
     7081 gcaggatatt ggctgcgcgc acggcctggg ccggcgcgcc cggctcggcg gcgtcggtcg
     7141 ggcttggcag gcggccgccc tccgacagca ggatttcgcc gaagcgaccc agggtgaagg
     7201 tgtcggtcac ggtcagttcc tgcgggaagg tgacgcgcat cccctcgaac ttctcgtaat
     7261 cggtcagggc cgtcaccggc agggtgatgg aagccgccgt gggcagcgtg ttgccgctgg
     7321 agcagacttg ggtgttgcta accgaggtaa gctcggtcag gttattaaac tcgtctaccg
     7381 tgcccacgac gcgcaccttg tcacccaggg ccacgttggt ggctgggttc tggccgtcga
     7441 agacgaagat gccctcggag gtgttgggat cggcgtcaac ctgggcgtcc tcttcctgaa
     7501 ggaagtagcc gctgatctca ttgggcgggt cggtggtgta atcctggaag tcgccgacga
     7561 cgatgccctc gatggtgcgg acgacgccgt cgtccgggct ggccgcgccg ttgccctgga
     7621 cgtcgtggat gggcgtcgcc gggtcgccac agacagcggg cggggtcgtg gtcgtgaagc
     7681 tgaacgtgta gtcggccgtc atgttgtcgg ccgtgccgtc ctggtcggcc accagcgtcg
     7741 actcgatggt gacggtgcag agttcaccgc cggtgaagtt gatgtcggga ttgagcgtga
     7801 acgttgtcgg cccgccgctg acggtggcgg tgtgcgtgcc ggagttggcg caggagatgg
     7861 tgtaccacgc gccgctgacg gtgaccgcct cgctgaacgt cacgctgatg tcggcagcca
     7921 gggcgacgtt ggtggcgctg ttggccgggg tggtgctggt gacggtgggg gccacgtcgc
     7981 cgccgcagac attcaacgtc gaggatgtgt tgcgcgggtt aggcgtacca ttggcgaagt
     8041 cggtactatt gttgtcggtc tcggtgcaac cacttccggc gcgtatcgcg gcagtcgtgg
     8101 cggtaaggtt aggagttgtg ggtgcagtct ccgagcagtt cgtggccggc ccaaaaccga
     8161 cgaggtcaac tacgtttgta ctagggcagg ctgtaccgct tgtaataaca gtggtagtac
     8221 tcattaacgc cactttacca gcgctaccgc tcatgttaat gccggtggct gtatggtctg
     8281 gcgtcggcag agcaatccca ttagctccac cactggccat ctgcacgaga tagtactgac
     8341 ctggcgcaat agacacattg gggaggtcgg ttttattcgt ccaacttgtt ccagctgtag
     8401 cagcatattg gattgaccat ccgttgatgc tcaccgaagt cgtgccgcgg ttaaaaagct
     8461 caacgaagtc gttggtataa gtagcgccac cattgccgcc accgccatat acctggctga
     8521 tgacgacgcc tgttgatagc gccgacgccg gcgcctggcg cgcggcaaac gcgaccagcg
     8581 ccaacatgag taaaagggct acacgaatga gcgtgcggga tcgggacatg ggtcagtatt
     8641 ctcctaacga caaggatagt ggtaaggacc cggcgctgcg cgggcgaccg acgccggacg
     8701 gctgttaact ccccttcccc cttctttacc ccttggggac aacccgttcg tagtgtacca
     8761 aagtaccaag caaatgcacg aaactgagca agggataaat tgacgggggc cgttttgact
     8821 aggggttagg agctagggat taggggctag ggaagaaggg aacgccgcgg tagtagtaca
     8881 ggctgttagc ctgtcgccgg caggctaaca gcctgcgcta cggctggctg tcagacccac
     8941 caaatacggt atacttcccc ccatgaacga ccagatcacc gaccacctgc gccgcgccta
     9001 cgacgccaaa gccgaggcgc gcaacgccgg cacgcaaagc tcgtggaagc tggtcgagcg
     9061 ggcggcgttc ctggagcaac tgcgggcggc cggggcgcga cgattgctgg agatcggcgg
     9121 cgggccgggc cacagcagcg ccttcttccg cgatgccggt cttgacgtgg tcatgaccga
     9181 cctctcgccg gagatggtgc gcctggcccg cgccaagggg ctggacgcgc gggtgatgga
     9241 cttccgccgg ctcgacttcc ccgacgcctc cttcgacgcc gtcttcgccc tcaactgcct
     9301 gctccacgtg ccctcggccg agctgccgcg cgccctggcc gaggtgcggc gggttttaca
     9361 gcccaacggg ctgttttact atggcgtcta cggcggctat tcgttcgagg gcgtctttgc
     9421 cgacgacgct tacgaaccga agcgctactt cgtcttctac cccgacgacg aactgcgccg
     9481 ccgcgccgcc gccgcgggct ttgacgaggt gtcgttccgc accatccccc ttgagggcga
     9541 cgacctccac tttcaggagc ttgtcctgcg gccggaggag cgcgtctgat ggtcgtcatt
     9601 ggtgacatcc acggccagta tgtgaagatg ctcaccgtcc tgcacgacgc gcggctgatc
     9661 gacgccgccg gtcactggtc gggcggggcg acgacgctgt ggttcatggg cgactacttc
     9721 gaccgcggcc ccgacggcat cgccgccatc gacctcattc gcgccctgca ggacg
//
LOCUS       DAOSMQ010000058         9612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7077_length_9612_cov_4.877263, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000058 DAOSMQ010000000
VERSION     DAOSMQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7077_length_9612_cov_4.877263"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..933
                     /locus_tag="PK829_03220"
     CDS             25..933
                     /locus_tag="PK829_03220"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOU40258.1"
                     /translation="MSSARRMHVDEVDTDVALVGRLLAAQFPRWAGLPLAPVASAGTD
                     NAIYRLGDDLVVRLPRIHWAAGQAAKEAAWLPRLAPRLPLAVPELLAIGRPGAGYPWP
                     WGVYRWLPGQQATLAALSDPCAAAAELARFLLALQRLDPTDGPDARAHGLRGAPLASR
                     DAGVRAAIAALDGLIDTDAATAVWSEALRAPEWDRAPVWFHGDFLPGNVLVSDGRPSA
                     VIDFGGLGVGDPACDLMIAWGLLAGESRAAFRAALAVDDATWARGRGHALAQAVIFIP
                     YYLETNPAGVAMARRALAEVLAEPVE"
     gene            1266..1487
                     /locus_tag="PK829_03225"
     CDS             1266..1487
                     /locus_tag="PK829_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40259.1"
                     /translation="MAYAEPRADDRRQVEAMLARLPFDLLYARLDVVRLDGRLALMEL
                     ELIEPMLYFDKAPHSARRLARATAARLAG"
     gene            1695..2048
                     /locus_tag="PK829_03230"
     CDS             1695..2048
                     /locus_tag="PK829_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005492236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU40260.1"
                     /translation="MTEKELDEQFDNLRLELRRGVVVLAVLSQMDTARYGYNLIQRLA
                     EQGLDVEEGTLYPLLRRLEKQGLLESEWEMGEARPRKYYRISPLGRKVLAALTTEWND
                     TVEVMHLILYGEKHV"
     gene            2041..3033
                     /locus_tag="PK829_03235"
     CDS             2041..3033
                     /locus_tag="PK829_03235"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HOU40261.1"
                     /translation="MSELVERYIHQVGRYLPSKERAEIQAELRSQIQDQLDDRYEGAP
                     TEANVAEVLKELGDPRRMAASYGGEQYLVGPDLYPVMMMVLRRGWVIVPPIAILVHLL
                     AVVFGDESGTLISLLLTAAGGAFQATAIFTGIVVLIFAILQHSGEDLDEITGSDKGFD
                     PLALPEVDDPAEVDRFESILSMAFGTFALLVMLYFLRVGGLTLRFNLNDPGDVIPVPI
                     PWLVALIAIGTTQMVLQLTALLRGRWGVALWLVNVALDIVGAVTLYYVLWLPLAGQLF
                     AAVPALLNVPFLGRGAQIILAISIIAAVLEGATKLVKMAGYRHSGPSSYNVKAS"
     gene            3135..4103
                     /locus_tag="PK829_03240"
     CDS             3135..4103
                     /locus_tag="PK829_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOU40262.1"
                     /translation="MKAIIYTRYGSPDVLQLKETAAPQPGEGEVLVKVWATGLNAADR
                     YALSGKPFIARLMIGGPRRPKHTILGADVAGVVEAAGSNVSRFRPGDAVYGDLSGVGS
                     GGLAEYVCAPEGVWALKPAGVSFEQAAAVPMAAVTALQGLRDKGQIRAGQRVLVHGAS
                     GGVGTFALQIAKALGAEVTAVCSPRNVDMAYALGADHVIDYTREDFAQNKQRYGLIFV
                     ANGNRPLGDYQRALAPGGIGVVAGGALGQIFQAMLLGPLVAGRGKVTSLSAKPDSGDL
                     ATIGEWLEKGTIRPVIDRCYPLAETAEAFRYLEKEHARGKVVVVQG"
     assembly_gap    4105..4204
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4205..4879
                     /locus_tag="PK829_03245"
     CDS             <4205..4879
                     /locus_tag="PK829_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU40263.1"
                     /translation="PDLIGLQECRDDEQAEYVRAALPDYEFFGVARGGNGPTALEMAP
                     VLWRRASFDLRQQGVFWLSETPDIPGSVGWDALFPRTATWVELAHRPSGQPLVFLNTH
                     FDLMPQAIIDSARLLYGWAERTAARRPLVITGDFNAGKTSAAYAILADGTMLVDAWRR
                     ANPAGGDETTYHDFGRPEAAAAIDWVLASPALAVAAAQIDRARPDGRYPSDHDPVVVT
                     LAATHF"
     gene            5046..5525
                     /locus_tag="PK829_03250"
     CDS             5046..5525
                     /locus_tag="PK829_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOU40264.1"
                     /translation="MKALVVYDSQYGNTERIAQAIGAALAARVETRVVRAADATADTL
                     ADCGLLVVGSPTQSFHATKPVDELLKGRSLRGVRAAAFDTRFDMAVVDSRMLRLAVRV
                     AGDNAYAAPRIAAQLAEAGAEVVAEPEGFIVTDTEGPLRAGELERATAWGTSLLATV"
     gene            complement(5522..6100)
                     /locus_tag="PK829_03255"
     CDS             complement(5522..6100)
                     /locus_tag="PK829_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU40265.1"
                     /translation="MKSASVAKSKLFQTAAELFYRHGYRATGVDTIAAESGIGKMTLY
                     RYYPSKDDLIVAYLRDSDEVFWSGFEQITAGATTARDKLLVFFKSLQDYATTPACYGC
                     PFLNVATEYPDGNYAGHQVAVAHKQAARERFRALAAEAGAARPEALADALHLLMDGAY
                     MAARLYNGAAGNPAAHLAETARQLIDAHCPAG"
     assembly_gap    6317..6416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6497..7462
                     /locus_tag="PK829_03260"
     CDS             6497..7462
                     /locus_tag="PK829_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU40266.1"
                     /translation="MNGTQPLGRSDVQVPRLGMGVMTWGHATGLARFHPAKLAYGGAE
                     EGEEEARAVQTSIAAGVNLFDTAAMYSGGASERRLGELTRGNPAALIATKFPGSMRAS
                     AADMPAELEASLRRLGRDTIDLYQHHFPTNRVDIPRLMNLMADAVAAGKIRAVGVSNY
                     SAEQMRLAHAVLAERGVPLASNQVEYSLLHRQPETNGVLDACRELGITLIAYTPLAGG
                     ALTGKYAAGDRPKGLRRFMMRQFRGRGLAEIAPVVALLREIGAAHDAQPAQVAIRWLI
                     ENECVLPIPGAKNSRQAAANAGALSFRLAPAEVDALDRATLAWRN"
     gene            7668..9566
                     /locus_tag="PK829_03265"
     CDS             7668..9566
                     /locus_tag="PK829_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOU40267.1"
                     /translation="MFRKALILMMAAMLALLLLAGCQSATMTTPISSATTASVTTTTD
                     TTTAATSAATSDTAASADASVSASEALEENGEIEVVSVDNVSAPAAVEGDAIAIALNG
                     DSISADSAAVVIDGPTATITAAGVYSLSGTLADGQIIVETEDEAVVTLVLNGVDITSS
                     TSAPIAVMSAEEVALVLADGTTNTLTDAASYVYPSADVDEPNAALFSNADLTISGNGA
                     LTVNGNYNDAISSDDGLVIAGGTLVVNAADDGIRGKDYLAIQGGNITVTAGGDGLKAS
                     EDTDATLGYISVSGGVIQITAAGDAITAETDVIISDGQFTLVAGGGSGSVVAADASAK
                     GIKGLVSVVIDGGTFAIDSADDAIHSNADITINGGTFSIATGDDAVHADTSLTVNGGA
                     IDVTTSYEGLESGIITLNAGDIHVVASDDGINVAGGTDSSGMMAGPGRGGRGGPGGAQ
                     NLDATTYTGSNFMYINGGTIVVDANGDGLDANGAIIMTGGLVVVNGPTNDGNGAMDYD
                     GGFNISGGTIVTAGSAGMLQSPDAASAQNSALIYVGQAAAGTLIHIQNSAGEDVLTFA
                     PTKSYAAVTLSSAELVSGETYTVYVGGSATGTANGGLYSDGAYTPGTELGSFTVSSAV
                     TTLGMGLR"
BASE COUNT         1550 a         3077 c         3213 g         1572 t
ORIGIN      
        1 ccgcggccat gatctaggcc gcccatgtct tccgcccgcc gaatgcacgt cgacgaggtc
       61 gataccgacg ttgccctcgt cggccgcctg ttggcggcgc agttcccgcg gtgggccggg
      121 ctgcccctcg cgccggtcgc gtcggccggc accgacaacg ccatctaccg cctgggcgat
      181 gatctggttg tgcgcctgcc gcgcatccac tgggcggccg ggcaggcggc caaggaggcg
      241 gcgtggctgc cgcggctggc cccgcggctg ccgctggccg tgccggagtt gctggccatc
      301 ggccggcccg gcgcgggcta cccctggccg tggggcgtct atcgctggct gccgggccaa
      361 caggcgacgc tggccgccct cagcgatccg tgcgccgcgg cggccgagct ggcccgcttc
      421 ctgctggccc tgcaacgcct cgaccctacg gacggccccg acgcccgcgc ccacggcctg
      481 cgcggcgcgc cgctggccag ccgcgacgcc ggcgtgcgcg ccgccatcgc cgcgctggac
      541 ggcctcatcg acaccgacgc ggcgacggcc gtgtggagtg aggccctgcg cgccccggag
      601 tgggaccgcg cgccggtctg gtttcacggc gacttcctgc ccggcaacgt gctagtgagc
      661 gacggccgtc ccagcgcggt catcgacttc ggcggcctgg gcgtcggcga cccggcttgc
      721 gacctgatga ttgcctgggg gttgctggca ggcgagagcc gggcggcctt ccgcgccgcg
      781 ctggcggtcg atgacgccac ctgggcgcgc ggacgcggcc acgccctggc gcaggcggtc
      841 atcttcatcc cttattacct ggaaaccaac ccggccggtg tagcgatggc gcggcgagcg
      901 ctggccgaag tgttggctga gccggtggaa tgagcgcgcc agatggctca ccgtccggcc
      961 acgtggaggc tgtctcgcca tgaacatcgc cgtcttgcgc tgtcgcaagc tgcccaactt
     1021 cgtcacctgg gagatccccg acgtggagga attgttcacc gacgaccggc tgctcatggc
     1081 cgcctttgcc gcggagggtc acgccgccga atccgtggcc tggggcgatg ccgtggagtg
     1141 gggccgcttc gatgccgccg tcctgcgtag cacgtgggac tatgtcgatg agagtgagcg
     1201 gtttttggca gcgctggcga ccgtcgaggc atcctcttgc cggctgttca atccgctgga
     1261 gagtggtggc ctacgccgag ccgcgcgccg acgaccggcg gcaggtcgag gcgatgctgg
     1321 cccggttgcc gttcgatctc ctctacgcgc ggctggatgt cgtgcggctg gacggccggc
     1381 tggcgctgat ggagctggaa ctgattgagc cgatgctcta tttcgacaag gccccccaca
     1441 gtgccaggcg gctggcccgc gctactgctg cccgccttgc cggctgacag ccagcgcact
     1501 cattgccacc ctgccagacg ttgccgctac aagcgcgccg tcatctccat cgccttcccc
     1561 caattgctgt cgccagaata atactgggta cttgacaata ctgtgcgtcg tatggtattc
     1621 tattactgtg ttacgcacag cataatgaga tgcatagtac tggcggacac acagcacaag
     1681 aagggacgac gcttatgact gaaaaggaac tagacgagca gttcgacaac ctgcgcctgg
     1741 agctgcggcg tggcgtggtg gtgctggctg tcctcagtca gatggacacc gcccgctacg
     1801 gctacaacct catccaacgc ctggccgagc agggcctgga cgtggaagag ggcacgctct
     1861 acccgctgct gcggcggctg gagaagcagg gcctgttgga gagcgaatgg gagatgggcg
     1921 aggcgcggcc gcgcaagtac taccgcatca gccccctggg gcgcaaggta ctggccgcgc
     1981 tgacgacaga gtggaacgac acggtcgagg tgatgcacct catcttgtat ggagagaaac
     2041 atgtctgagt tagtggagcg ctatattcat caagtggggc gctatctgcc cagcaaggag
     2101 cgggccgaga ttcaggccga actgcggtcg cagattcagg atcagctgga tgaccgctat
     2161 gagggcgcgc cgacggaggc caacgtcgcc gaggtgttga aggaactggg cgacccgcgc
     2221 cgtatggccg cttcctatgg cggcgagcag taccttgtcg ggccagatct gtatccggtc
     2281 atgatgatgg ttctgcggcg cggctgggtc atcgtgccgc cgattgccat ccttgtccac
     2341 cttctggccg tggtgtttgg cgacgagtcg ggcacgctga tcagcttgct attgaccgcc
     2401 gccggcggcg ctttccaggc cacggctatc ttcaccggca tcgtcgtgct cattttcgcc
     2461 atcttgcagc actccggcga agacctggac gagatcaccg gcagcgacaa gggcttcgac
     2521 ccgctggccc tgccggaggt agacgatccg gccgaggttg accgcttcga gtccatcttg
     2581 agcatggcct tcggcacgtt cgccctgctg gtcatgctct acttcctgcg cgtcggcggc
     2641 ctgacgctgc gcttcaacct gaacgatccg ggcgacgtca tcccggtgcc cattccctgg
     2701 ctggtggcgc tcatcgccat cgggaccacg cagatggtgt tgcagctgac ggcgctactg
     2761 cgcgggcgct ggggcgtggc tctgtggctg gtcaacgtgg ctctggacat cgtcggcgcg
     2821 gtgactctct actacgtgct atggctgccg ctggccggcc agctgttcgc cgccgtgccg
     2881 gcgctgctca acgtgccgtt cctgggccgc ggcgcgcaga tcatcctggc catttcgatc
     2941 atcgccgccg tgctcgaagg cgccactaag ctggtcaaga tggccggcta ccgccacagc
     3001 ggcccgtcat cctacaacgt gaaggcaagc taatcgcgcc gccagccaca gttacaccga
     3061 tgacgccgct gcctttgccg gcggtctaac tgccggtgta actgtggccg tcattcatct
     3121 ggacaaggaa actcatgaaa gcaattatct acacccgata cggttcgcca gacgtactcc
     3181 aactgaagga aacggccgcg ccgcaaccag gcgagggcga agtgctggtg aaagtgtggg
     3241 cgacgggcct caatgctgcc gaccggtatg cgctgagcgg caagccgttc atcgcccggc
     3301 tcatgatcgg cgggccgcgc cggccaaagc ataccatcct gggggccgac gtggccgggg
     3361 tggtcgaagc cgccggctcg aatgtgagcc gcttccggcc gggcgacgcc gtctatggcg
     3421 atctgtccgg cgttggttcg ggcggcctgg ccgagtacgt ctgtgcgccg gagggcgtct
     3481 gggcgctgaa gccggcgggc gtcagctttg agcaagccgc ggccgtaccg atggccgccg
     3541 tcacggccct gcaaggtctg cgcgacaagg ggcagattcg cgccgggcag cgggtattgg
     3601 tccatggcgc gtcgggcggc gtgggtacgt ttgcgctgca gatcgccaaa gccttggggg
     3661 ctgaagtgac ggccgtgtgc agcccgcgca acgtggacat ggcttatgct ttgggggccg
     3721 atcacgttat tgattacacg cgcgaggact ttgcccaaaa caagcagcgc tatgggttga
     3781 ttttcgtcgc caacgggaat aggccgcttg gtgattatca gcgggcgttg gctcccggcg
     3841 gtattggcgt ggtcgctggc ggggcgctgg gccagatctt tcaggccatg ctgctggggc
     3901 cgttggttgc tggccggggc aaagtgacaa gtctgtcggc caagcccgat agcggcgacc
     3961 tggccaccat tggcgaatgg ctggaaaagg ggacaatacg gccggtgatt gaccgctgct
     4021 acccactggc cgagacggcc gaggcatttc gctacttaga gaaggagcat gcgcggggta
     4081 aggtggttgt tgtgcagggg tagannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnncccgat ctgatcggcc tgcaggagtg ccgcgacgac gagcaggccg aatacgtccg
     4261 cgcggcgctg cccgattatg aattcttcgg cgtggcgcgc ggcggcaacg ggccgacggc
     4321 gctggagatg gccccggttc tgtggcggcg ggcgtcgttc gacttgcggc agcagggtgt
     4381 cttctggctg agcgagacgc cggacattcc cggcagcgtg ggctgggatg ccctgttccc
     4441 gcgcacagcc acctgggtgg aactggccca ccggccgagc ggccaaccgc tggtctttct
     4501 caacacccac ttcgatctca tgccgcaggc catcatcgac tcggcgcggc tactatatgg
     4561 ctgggccgag cggacggcgg cgcggcggcc gctggtcatc accggcgact tcaacgccgg
     4621 caagacctcg gccgcctacg ccatactggc tgacgggaca atgctggtcg atgcctggcg
     4681 gcgcgccaac ccggccggcg gcgacgagac gacctaccac gacttcggcc ggccagaggc
     4741 cgccgcggcc atcgactggg tgctggcgtc gccggcgctg gccgtggccg cggcgcaaat
     4801 cgaccgcgcc cgcccggatg ggcgctaccc gtcagaccat gacccggttg tcgtcacgct
     4861 ggcggcgaca cacttctaac atcttcttca cactggctga acgttcgggt cttataaggg
     4921 aggcacacac ggccagcctg actttgaacg agcgggagca taggttcccc tcggtcgagc
     4981 aaaccgtggc gagacgacca gattgtcgcc gcgccgcggg cgtaccatcg ggaggtcgca
     5041 ctctgatgaa agcattggtc gtttacgatt cgcaatatgg caatacggag cgcatcgccc
     5101 aggccatcgg cgcggcgctg gcggcgcggg tggaaacccg ggttgtgcgc gcggccgacg
     5161 cgaccgccga caccctggcc gactgtgggc tgctggtcgt cggttcgccg acgcagtcgt
     5221 tccacgccac caagccggtc gatgagttgc tgaaagggcg ctcgctgcgg ggtgtccgcg
     5281 cggcggcgtt cgacacgcgc ttcgacatgg ccgtggtgga ttcgcgcatg ctgcgcctgg
     5341 ccgtgcgcgt ggccggtgac aatgcctatg ccgcgccgcg catcgccgcg caactggccg
     5401 aggccggcgc cgaagtagtc gccgagccgg agggcttcat cgtcacggac acggaaggcc
     5461 cgctacgcgc gggcgaactg gagcgagcca ctgcctgggg cacgagcctg ctggcaaccg
     5521 tctagccggc cgggcagtgg gcgtcgatga gctgtcgcgc cgtttcggcc aggtgggcgg
     5581 ccgggttgcc ggccgcgccg ttgtagagcc gcgcagccat gtaggctccg tccatgagca
     5641 agtgcagcgc gtcggccagc gcctccggcc gggccgcgcc cgcctcggcc gccagcgcgc
     5701 ggaagcgctc ccgcgcggcc tgcttgtggg ccacggcgac ctggtggccg gcgtagttgc
     5761 cgtcggggta ctccgtggcc acgttgagga aagggcagcc atagcacgcc ggcgtcgtgg
     5821 cgtagtcctg caaggacttg aagaagacca acagcttgtc ccgcgccgtc gtcgccccgg
     5881 cggtgatctg ctcgaagccg ctccagaaga cctcgtcgct gtcgcgcaga taggcgacga
     5941 tgagatcgtc tttcgatggg tagtagcggt agagggtcat cttgccgatg cccgattcgg
     6001 cggcgatggt gtctacgccc gtggcccggt agccgtggcg gtagaacagc tcggccgctg
     6061 tctggaaaag cttgctcttg gcaacacttg cgctcttcat cactgattcc ctcttgacaa
     6121 gatacagatc tgtattgtat catacctgtc aaatgataca gaactgtatt gtgtgtggcg
     6181 cgattctctg aatcgcgtta cgggattgcg attgcgattc gagagaatcg cgccacggga
     6241 ttgcgattcg gagaatcgcg ccacgggatt gcgattcgag agaatcgcgc cacgggattg
     6301 cgattcggag aatcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaat
     6421 cgcgccacgg gattgcgatt cgagagaatc gcgccacggg attgcgattc gagagaatcg
     6481 caccacgagg atgaaaatga acgggacaca acctttggga cgcagtgatg tgcaggtgcc
     6541 gcggctgggt atgggcgtga tgacctgggg gcatgccacc gggctggccc gcttccatcc
     6601 ggccaagctg gcctatggcg gcgcggagga gggcgaggaa gaggcccgcg ctgtgcagac
     6661 gagcatcgct gccggcgtga atctcttcga cacggcggcc atgtacagcg gcggcgcgtc
     6721 ggagcggcgg ctgggcgagt tgacccgcgg caacccggcg gccctcattg ccaccaagtt
     6781 ccccggctcc atgcgcgcca gcgccgcgga catgcccgcc gaactggaag ccagcctgcg
     6841 ccggttgggg cgggacacga ttgacctcta ccagcaccac ttcccaacca accgggtaga
     6901 catccctcga ctgatgaatc tgatggccga tgccgtcgcc gcgggcaaaa tccgcgccgt
     6961 gggcgtcagc aactactcgg ccgagcagat gcgcttggcc cacgctgtcc tagccgagcg
     7021 cggcgtgccg ctggcttcta accaggtgga gtattcgctg ctccaccgcc agccggagac
     7081 caacggcgtg ctggacgcct gccgcgagct gggcatcacg ctgatcgcct acacgccgct
     7141 ggccggtggg gcgctgacgg gcaagtacgc cgccggcgac cggccgaagg ggctgcggcg
     7201 gttcatgatg cgccagttcc gcggccgggg gttggccgag atcgccccgg tcgtggccct
     7261 gctgcgcgag attggcgcgg cccacgacgc ccagccggcc caggtggcca ttcgctggct
     7321 gattgagaac gagtgcgtcc tgcccatccc cggcgcgaag aacagccgcc aggcggccgc
     7381 caacgccggg gcgctgtcgt tccggctggc gccggccgag gtggacgcgc ttgaccgggc
     7441 gacgctggcc tggcgcaact aaggctccct ccgggcgcgg gcgtgagtgg cctgcccgcg
     7501 cccattacac ttcgctgact tctccttcac aatttctgaa cgatcctgcc ctataaaaga
     7561 gagtgttggg tagcgccccg cgccccggtt cgagtggcgg cgaggcggca agagcgccca
     7621 gacacgcgaa atcacaccgg aagtgataac cggttaggag caagatcatg ttcagaaaag
     7681 cacttatttt gatgatggcc gctatgctgg cgctgttgtt gctggccggc tgccagagcg
     7741 ccacgatgac gacgcccatc tcatcggcga cgaccgcgag cgtcaccacc acgacagata
     7801 ccacgaccgc ggccaccagc gccgccacgt cggacacggc cgccagcgcg gacgcctccg
     7861 tttccgcgtc cgaggcgctg gaggagaatg gcgagattga agtcgtcagc gtggacaacg
     7921 tgagcgcgcc ggccgccgtc gaaggcgacg ccatcgccat cgccctcaac ggcgacagca
     7981 tcagcgccga cagcgccgcc gtcgtcatcg acggccccac ggcgaccatc accgctgccg
     8041 gcgtctatag cctcagcggc acgctggccg acggccagat catcgtcgag acggaagatg
     8101 aagctgtcgt cacgctggtg ctcaacggcg ttgacatcac cagttccacc agcgcgccca
     8161 tcgccgtgat gagcgccgag gaagtggccc ttgtcctggc cgatggcacg accaacaccc
     8221 tgaccgacgc ggccagctac gtctacccca gcgcggacgt ggacgaaccc aacgccgccc
     8281 tcttcagcaa cgccgatctg accatcagcg gcaacggcgc gctgacggtc aacggcaact
     8341 acaacgacgc catctccagc gatgacggcc tggtgattgc cggcggcacg cttgttgtca
     8401 acgcggccga tgacggcatc cgcggcaagg actacctggc catccagggc ggcaacatca
     8461 ccgtgacggc cggcggcgac ggtctgaagg ccagcgaaga caccgacgcg acgctgggct
     8521 acatctctgt cagtggcggg gtgattcaga tcacggccgc gggcgacgcc atcaccgccg
     8581 agacggacgt gatcatcagt gatggccagt tcacgctcgt cgccggcggc ggcagcggca
     8641 gcgtggtcgc cgccgacgct tcggccaagg gcatcaaggg cctggtcagc gtcgtcatcg
     8701 acggtggcac gttcgccatc gacagcgccg atgacgccat ccactccaat gccgacatta
     8761 ccatcaatgg cggcacgttc agcatcgcta ccggcgacga cgctgtccac gccgacacct
     8821 ctctgacggt caacggcggc gcgatcgatg tcaccacctc gtatgagggc cttgagagcg
     8881 gcattatcac ccttaacgcc ggcgacatcc acgtcgtcgc cagcgatgac ggcatcaacg
     8941 tggccggcgg cacggatagc tccggcatga tggccgggcc ggggcgcggc ggtcggggtg
     9001 ggccgggcgg cgcgcagaac ctggacgcca ccacctacac cggctcgaac ttcatgtaca
     9061 tcaacggcgg cacgattgtg gtcgatgcca atggcgacgg cctggacgcc aacggggcca
     9121 tcatcatgac cggcggcctg gtcgtcgtca atggcccgac caatgacggc aatggggcga
     9181 tggactatga cggcggcttc aacatcagcg gtggcaccat cgttaccgcg ggcagcgccg
     9241 gcatgctcca gtcgccggat gccgcctcgg cccagaactc cgccctcatc tacgtcggtc
     9301 aggccgccgc cgggacgctc atccacatcc agaacagcgc cggcgaagat gtactcacct
     9361 tcgcgccgac caagagctac gccgcggtga cgctctcctc agcggagttg gtcagcggcg
     9421 agacctacac cgtctacgtt ggcggcagcg ccacaggcac ggccaacggt ggcctctaca
     9481 gcgatggcgc gtacacgccg ggcacggaac tgggcagctt cacggtgtcg agcgccgtga
     9541 cgacgctggg tatgggcctg cgctaaggcg tatgggcctg cgctaaggcg tatgggcctg
     9601 cgctaaggcg ca
//
LOCUS       DAOSMQ010000059         9497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7176_length_9497_cov_5.179941, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000059 DAOSMQ010000000
VERSION     DAOSMQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7176_length_9497_cov_5.179941"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="PK829_03270"
     CDS             complement(<1..1550)
                     /locus_tag="PK829_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40268.1"
                     /translation="MRRIVYRLWKPACLGVLLALLAGGVSFLAAAPAEPEASYKLSSD
                     GPLPIGGQVNNFLISPNGAYTVYWAGQDITEIAQLYATRTDGSGVPVRLSALPQAGQY
                     IEGYNISPDNARVVYIADQDTRNVIELYSAPLNGSAAAVKLNRPLVAGGSLPDNSSSF
                     QISPNSRRVVYRADGDTDEISELYSVPIDGSAAAVKLNGPLVAGGEVYQFAITPDGAS
                     VVFTADKNTGNVIELYRVPIDGSAAPVKLSGALVAGGGVFDFKLSPDSLFAVYRADAS
                     TNDVMELYSVLLDGSAMPVKLNGPLAPGGEVAFGGTYVISPDNERVIYLADQDTNDVD
                     ELYSVPINGGLVVKLNPPLVPGGDVEWYPVPQISPDSSRVVYAADQETNDVYETFSAP
                     LAGGGMSVKLNGPLTGNYADDFAISPDSGRVVYRALQDSATQYELYSVPLAGGAPVKL
                     NSPLGGTGQVNDFTVSPDSSRVVYMASQDAASGSELYRVPLAGGAASKVNGALPPGGA
                     VSNYDISRA"
     gene            complement(1789..2958)
                     /locus_tag="PK829_03275"
     CDS             complement(1789..2958)
                     /locus_tag="PK829_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOU40269.1"
                     /translation="MKRLLWIAAALAVIVVAAVASRPLWARQAAPPLAASLEALPAAG
                     AIDGFARATQPGAIVFPRDLGPHDDYQTEWWYYTGNLTTADGRPFGFQFTIFRRALTP
                     PGDGDTAAADGWRSNQVYLAHFAISDIAADAFYPAERFSRGAAGLAGATATPYRVWLE
                     DWSVEEIAAGQVRLRARTDAVALDLILTQTLPPVLHGDGGLSQKGPEAGNASYYYSIV
                     QQAAAGTVTAGGEAFDVTGRAWKDHEWSTSALSEGAVGWDWLSLQLDDGSALMLFEIR
                     RADGTREAFSAGSYIAPDGTLTPLALGDWTLAVTDTWTSPTSGAEYPAGWRITIPSLG
                     LELTGAPQMDNQELNLSTVYWEGAVAFSGTRDGAPVSAQGYIELTGYAGSMEGRL"
     gene            complement(2976..>3392)
                     /locus_tag="PK829_03280"
     CDS             complement(2976..>3392)
                     /locus_tag="PK829_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40270.1"
                     /translation="IFDRTFAITSALRLLAVIVAFIGVLSALMSLQLERARELGTLRA
                     TGMTRRQLWGLTLLETGLMGTTAGLLALPVGYGLAWILVYVINVRSFGWTLSMALRPE
                     YFLQAFLVAVSAALLAGIYPALRLGNLEIAAAIREE"
     assembly_gap    3393..3492
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3493..5273)
                     /locus_tag="PK829_03285"
     CDS             complement(<3493..5273)
                     /locus_tag="PK829_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40271.1"
                     /translation="MSNTPLYRLAWRRARRHALQYLLFILGVAIGVAMMVSIDLANGS
                     ARRAFALSTDAITGKASHRIAAGPDGLDEDVYRRIRVEAGFAPAAPVVEGYVGVPALG
                     GQPVRLVGIDPFAEPPFRDYFGPAGGANGGALDPNALTAFLTEPNSVILSADAAAANG
                     IALGDTLTVDVAGRRIPARVVGLLRPADDVTRRALGGIIFTDIASAQEMLDLRGRLSH
                     IDLIVPANAAGEAALAAVAAVLPAGATIETAAARQNAIQQMTAAFEFNLTALSLLALV
                     VGMFLIYNTVTFSVVQRRPLFAVLRMLGATGGQLFVLIVGEAALLSLVGSALGLGLGV
                     LLGRGVVGLITQTINDFWFVVNVQGVSIAPLSLARGLAVGVGAAVAAALVPAIEAART
                     PPQSTLRRSTLESRVRRLLPWLVVAWAVLTLAAVALLAAGGGLATAFGGLFAVLIAAA
                     LLAPPVTAVLMRLVAPLSGRLLGVLGRMAPRDIVRSLSRTSIAVAALMTAVAVIVGVS
                     IMVGSFRGTVVQWLDQTLQADIYLSPPNVTANRVVGALAADVVATARTWPGVARAVTS
                     HTVQITLPDFDRLVQLVAVDGDVSNGNR"
     gene            complement(5315..5812)
                     /locus_tag="PK829_03290"
     CDS             complement(5315..5812)
                     /locus_tag="PK829_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40272.1"
                     /translation="MSKPARVVLLLLAVVVLSAAFVLARPRPAPILAAPNVFSGPVNG
                     GCYLQTVTTCAIHVDSWQPVVMDSGRALVGWQLAAQAQGASGSAVLYDFRTDVSNPPI
                     GSYLPSLVKQDFAAHCGVSYHLTLRAQDSGDLSFEEIGRTEVFACPAAPIAPRVYLPA
                     ALRGQ"
     gene            complement(5830..6279)
                     /locus_tag="PK829_03295"
     CDS             complement(5830..6279)
                     /locus_tag="PK829_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40273.1"
                     /translation="MRKRLAVTIFILLLAVVASLVARFASLDEASAAPAVPAGFASPI
                     NGGCYIAAPNQCKLHVDPFTININEGGGVVLEKFTLYANGSPIYDFRTDVSNPPYADY
                     APSLVMADFAAECGQTYYMNLVAKDSSDSSPLNYGQTAQFTCPAAVP"
     gene            complement(6462..7223)
                     /locus_tag="PK829_03300"
     CDS             complement(6462..7223)
                     /locus_tag="PK829_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40274.1"
                     /translation="MNETPTLSPPIPRPAVAPAPADAPPQPVVQLHALRKSFTEGGQS
                     RVVLDGVDYSFARGEFVVLLGHSGSGKSTLLNLISGIDRPDGGDITILDTPITRLSER
                     DRTLFRRDHIGFIFQFFNLIPTLSVLENVTLPQELAGRDRREVAPGAMRLLERVGLAD
                     RRDANPDKLSGGEQQRVAIARALAHDPLLVLADEPTGNLDDATGERVLALLLELTRDA
                     GRTLLMATHNPDIARRADRVLQLHDGRLLEVGHVQ"
     gene            complement(7313..7945)
                     /locus_tag="PK829_03305"
     CDS             complement(7313..7945)
                     /locus_tag="PK829_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOU40275.1"
                     /translation="MDEQIPDIVIGRLPVYLRELERMVRDAGKATTSSHELAERLGMS
                     SAQIRKDFSHFGEFGKQGTGYHIHYLIDRLTEILNLTHEWPVALVGAGFLGHALASYR
                     GFQRRGFVITCVFDNDPAKIGKTIGDLVVQDAAALETALRTRGIAVAILAVPADAAQE
                     MVDRLVAAGVRAILSYAPVSLTVPPGVRVSYSDPVVQLQQMAYYLSSTAS"
     gene            8285..>9497
                     /locus_tag="PK829_03310"
     CDS             8285..>9497
                     /locus_tag="PK829_03310"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU40276.1"
                     /translation="MSRTFRNILLVVLALAVVGAGVFWFVNRRQAAAAAQVEILREAT
                     VARDTITSTVNATGAIEPETLVTLTFGLSGTVTDVAVTRGQVVAAGDVLAQLDTSEPA
                     LAVQQAEDALRIQELTLAQRVEGGPSPAQLASSAADIQAAEANVQVAQANLSGAQAGV
                     QQAQAQRAQLLEGATPGQIAAAESQVAAAQQQQKQAEETHNKTLECVTFAVPGTNQQD
                     EVCPGLGEPEEAARAALETATLNLQAAQAQLADLQNPASAANIAAADAAVAAAAAQVE
                     AAEGNVAAAEAQLARARAANDLLREGPSAAEQAILDAQVASARTNLEIAQLRLKQAQL
                     IAPIDGQVASVLIRAGEQAAPGAPAMTVLNENAYHITVQVDEIDIDRVEAGQPALITL
                     DALPDKPVQGTV"
BASE COUNT         1539 a         3123 c         3342 g         1393 t
ORIGIN      
        1 gcgcggctga tatcgtagtt gctgacggcc ccgccgggcg gcagcgcgcc gttgaccttc
       61 gacgccgccc cgccggccag gggcacgcgg tacaactcgc tgccgctggc cgcgtcctgg
      121 ctggccatgt agacgacgcg gctgctgtcg gggctaaccg tgaagtcatt gacctgccct
      181 gtgcccccga gcgggctgtt gagcttcacc ggcgcgccgc cggccagggg cacgctgtag
      241 agttcgtact gggtggccga gtcctgcagg gcgcgataga cgacgcggcc gctatcgggg
      301 ctgatggcga agtcatcggc gtagttgccg gtcagcgggc cgttgagctt caccgacatt
      361 ccgccgccgg ccaggggcgc gctgaatgtc tcatagacgt cgttggtctc ctggtcggcg
      421 gcatagacga cgcggctgct gtcggggctg atctgcggca cggggtacca ctccacatcg
      481 ccgccgggca caagcggcgg gttgagcttg acgaccaggc cgccgttgat gggcacgctg
      541 tagagttcat cgacgtcgtt ggtgtcctgg tcggccagat agatgacgcg ctcgttgtcg
      601 gggctgatga catacgtgcc gccaaaggcg acctcgccgc cgggggccag cgggccgttc
      661 agcttaaccg gcatggcgct gccatccagc aacaccgagt acagttccat gacatcgttg
      721 gtgctcgcgt cggcccgata gacggcaaac aggctgtccg ggctgagctt gaaatcgaag
      781 acgccgccgc cggcgacgag cgcgccgctg agtttcaccg gcgcggcgct gccgtcgatg
      841 ggcacgcggt agagttcaat gacgttgccc gtgttcttgt cggccgtgaa aaccacgctg
      901 gcgccatcgg gcgtgatggc gaactgatag acctcgccgc cggccaccag cgggccgttc
      961 agcttcaccg ccgcggcgct gccgtcgatg ggcacgctgt atagctccga gatctcgtcg
     1021 gtgtcgccgt cggcgcgata gaccacgcgg cggctgttgg ggctgatctg gaagctgctg
     1081 gagttgtcgg gcaggctgcc gcccgcgacc agcggccggt tcagcttgac ggccgcggcg
     1141 ctgccgttca gcggggcgct gtatagttcg atgacgttgc gtgtgtcctg gtcggcgatg
     1201 tagacgacgc gggcattatc cgggctgatg ttgtagccct cgatatactg ccccgcttgc
     1261 ggcagggcgc tcagccgcac cgggacgccg ctgccgtccg tgcgcgtggc atagagttgg
     1321 gctatctcgg tgatgtcctg gccggcccag tagacggtgt aggcgccgtt gggactgatc
     1381 aggaagttgt tgacctggcc gccaatgggc agcgggccgt cgctgctcaa cttgtaagat
     1441 gcttcgggtt ccgccggcgc ggccgcgagg aacgagacgc cgccggccag cagggccaac
     1501 aatacaccca gacaagctgg cttccacagc cgatagacga ttcgccgcat gacggtctcc
     1561 ttactgtact gtgcagtttg atgtgtggtg gccaggctag acccgactga caagagaacg
     1621 cgcccgcttc agccgccggc cgcgtggcgc gacgagagaa gtcccctggt caggatcata
     1681 gcatctggcg cggccgggcg aaacggccgt ttgctgcact tgtgccggag aagtctaggg
     1741 agaaatcgac agagcgcggc ggggtctggt acgccgccgg gcgataaact acagccgccc
     1801 ctccatcgac ccggcgtagc cggtcagttc gatgtagccc tgggctgaga cgggcgcgcc
     1861 gtcgcgcgtg ccgctgaatg ccaccgcccc ttcccagtag accgtggaga ggttgagttc
     1921 ctggttatcc atctgcggcg cgccggtcaa ctccagtccc agtgacggta tggtgatgcg
     1981 ccagccggcg gggtactccg cgccgctggt ggggctggtc cacgtgtcgg tcacggccag
     2041 cgtccagtcg cccagcgcca gcggggtcag cgtgccgtcc ggggcgatgt agctgccggc
     2101 cgagaacgcc tcgcgcgtgc cgtcggcgcg acggatctcg aacagcatca gggcactacc
     2161 gtcgtccagt tgcagcgaca gccagtccca gccgacggcc ccctcgctca gggcgctggt
     2221 gctccactcg tggtctttcc acgcccggcc ggtcacgtcg aacgcctcgc cgccggcggt
     2281 gactgtgccc gcggcggcct gctggacgat ggagtagtag taggaagcgt tgcccgcctc
     2341 cggccccttc tggctcagcc caccgtcgcc gtggagcacg ggcggcagcg tctgtgtcaa
     2401 tatcaggtcg agggcgacgg cgtcggtgcg ggcgcgcagc cgcacctggc cggcggcgat
     2461 ttcctccacc gaccaatcct ccagccagac gcgatatggc gtggccgtgg ccccggccag
     2521 cccggccgcg ccgcggctga agcgctcggc agggtagaag gcgtcggcgg cgatgtcgct
     2581 gatggcaaag tgggccaggt agacctggtt gctgcgccag ccgtcggccg cggccgtgtc
     2641 gccgtcgccc ggcggggtca gggcgcggcg gaagatggtg aactggaagc caaagggccg
     2701 gccgtcggcc gtggtcaggt tgccggtgta gtaccaccac tcggtctggt agtcgtcgtg
     2761 cggccccaga tcgcgcggga agacgatggc ccccggctgc gtggcccggg cgaagccgtc
     2821 gatggccccg gccgccggca gcgcctccag cgaggcggcc agcggcggcg cggcttgcct
     2881 ggcccacagc ggccggctgg ccaccgcggc gacgacgatc accgccagcg ctgcggcgat
     2941 ccataacaat cgtttcatgc tcatgatcct tagggctact cctcgcggat ggccgcggcg
     3001 atctccaggt tgcccagccg cagcgccgga tagatgcccg ccagcagcgc ggcgctgacg
     3061 gccaccagga aggcttgcag gaagtactcc ggccgcaagg ccatactcag cgtccagccg
     3121 aaggagcgca cgttgatgac gtagaccaga atccaggcca gcccatagcc gaccggcagc
     3181 gccagcagcc cggccgtcgt gcccatcagc cccgtttcca gcagcgtcag gccccagagc
     3241 tgccggcggg tcatgcccgt ggcccgcagc gtgcccagtt cgcgcgcccg ctccagttgc
     3301 aggctcatca gggcgctgag cacgccgatg aaggcgacaa taaccgccag cagccgcagg
     3361 gccgaggtga tggcaaaggt gcggtcgaag atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nncggttgcc gttgctgacg tcgccatcga cggccaccag ttgcaccagg
     3541 cggtcgaagt cgggcagggt gatctgcacc gtgtgcgacg tgaccgcgcg ggccacgccc
     3601 ggccacgtcc gcgccgtggc cacgacatcg gccgccagcg cgccgacgac gcggttggcg
     3661 gtgacgttgg gcggcgagag gtagatgtcg gcctgcagcg tctggtcgag ccattggaca
     3721 accgtgccgc ggaaggaacc aaccatgatc gacacgccga cgatgacggc cacagccgtc
     3781 atgagcgcgg ccacggcgat agaggtgcgg ctgagcgagc ggacgatgtc acgcggggcc
     3841 atgcggccga ggacgcccag cagccggccg ctgagcggag cgacgaggcg catcagcacg
     3901 gccgtgaccg gcggggccag cagcgccgcg gcgatgagga cggcgaagag gccgccgaag
     3961 gccgtcgcca gcccgccacc ggcggccagc agcgccaccg cagccagcgt cagcacggcc
     4021 caggcgacca ccagccaggg caacagccgc cgcacgcggc tctccagcgt cgagcggcgc
     4081 agcgtgcttt gcggcggggt gcgcgccgcc tcgatggccg gcacgagcgc cgcggccacg
     4141 gccgcgccca cgcccacggc cagcccccgc gccaggctca gcggggcgat gctgacgccc
     4201 tggacgttga cgacgaacca gaagtcgttg atcgtctgcg tgatcaggcc gacgacgccg
     4261 cggccgagca gcacgcccag ccccagcccc agcgccgagc cgaccaggct gagcagcgcc
     4321 gcctcgccga cgatgaggac gaagagttgc ccgcccgtcg cccccagcat ccgcaggacg
     4381 gcgaaaagag gccgccgctg gacgacgctg aaggtgacgg tgttgtagat gaggaacatg
     4441 cccacgacga gggccagcag actcagcgcg gtcaggttga actcgaaggc ggcggtcatc
     4501 tgctggatgg cgttctggcg ggccgcggcc gtctcgatgg tcgcgccggc gggcaggacg
     4561 gcggccacgg ccgccagggc cgcctcgccg gcggcgttgg ccgggacgat gaggtcgatg
     4621 tggctgaggc gtccgcgcag gtcgagcatc tcctgggccg aggcgatgtc ggtaaaaatg
     4681 atgccgccca gggcgcgccg ggtcacgtcg tcggccgggc gcagcaaccc gaccacccgc
     4741 gccggaatgc gccgcccggc gacatcgact gtcagcgtgt cgcccagggc gatgccgttg
     4801 gccgcggcgg cgtcggcgct caggatgacg ctgttcggct cagtcaggaa ggcggtcagg
     4861 gcgttggggt ccagcgcgcc gccgttggcc ccgccggcgg ggccgaagta gtcgcggaag
     4921 ggcggctcgg cgaaggggtc gatgcccacc agccgcaccg gctgcccgcc caacgccggc
     4981 acgccgacgt agccctcgac gaccggcgcg gccggcgcga agccggcctc gacgcggatg
     5041 cgccggtaga cgtcttcgtc cagtccatcg gggccggcgg cgatacggtg gctggccttg
     5101 ccggtgatgg cgtcggtcga gagggcgaac gcccgccgcg ccgagccgtt ggccaggtcg
     5161 atcgacacca tcatggccac gccgatggcc acgccgagga tgaacagcag atattgcagc
     5221 gcgtgccgcc gtgcccgtcg ccaggcgagg cggtagaggg gggtgttgct catgaggggg
     5281 acagtgggtc agtgggccag tggtcggccg tcgctcattg ccctcgcagg gccgccggca
     5341 ggtatacccg tggcgcgatc ggcgcggccg ggcacgcgaa gacctcggtg cggccaattt
     5401 cttcaaacga caggtcgccg ctgtcctggg cgcgcaacgt caggtggtac gaaacgccac
     5461 aatgggcggc gaagtcttgc ttcaccaggg agggcaggta gctgccgatc ggtgggttag
     5521 acacatcggt gcgaaagtca tagaggacgg cgctgcccga cgcgccctgg gcttgcgccg
     5581 ccagttgcca gccgaccagc gcccggccag agtccatgac gacgggttgc cagctatcca
     5641 cgtgaatggc gcacgtcgtc accgtttgca gatagcagcc gccgttcacc ggcccggaga
     5701 atacgttggg cgcggccagg atgggcgcgg ggcgcggccg cgccaggacg aacgcggccg
     5761 acagaacgac cacggccagc aataacaaga caacgcgcgc cggcttgctc atgatcggct
     5821 ctatcgctct cagggcacgg ctgccgggca ggtgaactgc gccgtctgcc cgtaattgag
     5881 cggtgaactg tcgctgctgt ccttggcgac caggttcatg tagtaggttt ggccgcactc
     5941 ggcggcgaag tccgccatga ccagcgacgg ggcgtagtca gcgtagggcg ggttcgacac
     6001 atcagtgcgg aagtcataga tggggctgcc gttggcgtag agagtgaact tctccaggac
     6061 aaccccgccc ccttcattga tgttgatggt aaacggatcg acgtggagct tgcactggtt
     6121 gggcgcggcg atgtagcacc cgccgttgat cgggctggcg aaaccggcgg gaacggccgg
     6181 cgcggcgctg gcctcatcga gcgaggcgaa gcgcgccacc aggctggcga ccacggccag
     6241 caacaagatg aaaatcgtga cggcaagtcg ttttctcatg tcaatctctc cggcgcagat
     6301 caatccacgc ggcgcggact tgcgctgagg cgctcggacg acgcgatttc agtgtaacag
     6361 aatcggttgc gcccgtaacg atacaaacga cccatttttg gccaagatga ccagccaacc
     6421 gtcgtcaaca ggcatgccgg ccacggcttg ctgctgaccg gtcattgaac atgacccact
     6481 tccagcaggc ggccgtcgtg gagctggagc acgcggtcgg cgcggcgggc gatgtcgggg
     6541 ttgtgggtgg ccatgagcag ggtgcggccg gcgtcgcgcg tgagttccag cagcagggcc
     6601 aggacgcgct cgccggtggc gtcgtcgagg ttgccggtcg gctcgtcggc cagcaccagc
     6661 agcggatcgt gggccagggc gcgggcgatg gccacgcgtt gctgctcgcc accggagagc
     6721 ttgtcggggt tggcatcgcg ccggtcggcc agacccaccc gctccagcag gcgcatggcc
     6781 ccaggcgcga cctcccggcg gtcgcgcccg gccaactcct gcggcagggt cacgttctcc
     6841 agcacgctga gcgtggggat gaggttgaag aactggaaga tgaagccgat gtggtcgcgg
     6901 cgaaagagcg tccggtcgcg ctcggacagg cgggtgatgg gcgtgtccag gatggtgatg
     6961 tcgccgccgt cgggccggtc gatgccgctg atgagattga gcagggtgct cttgccgctg
     7021 ccgctgtggc ccagcaggac gacaaactcg ccgcgggcga agctgtaatc caccccgtcg
     7081 aggacaacgc gcgactggcc gccctcggtg aagctcttgc gcagggcgtg aagctggacg
     7141 acgggttggg gcggggcgtc tgccggcgca ggggccacgg ccgggcgcgg aataggcggc
     7201 gaaagagtcg gcgtttcgtt catgccaacc agttatatcc caggcgcggc cggtcaacac
     7261 gtacagcgac gggcgtatca ccgaggctta aggcgctggg cgggcgagtt gtctaggaag
     7321 ccgtggacga gagatagtag gccatttgct gcaactgcac caccgggtcg ctgtagctga
     7381 cacggacgcc gggcggcact gttagcgaga ccggcgcata gctgaggatg gcgcgcacgc
     7441 cggcggcaac aaggcgatca accatttcct gggcggcgtc ggcgggcacg gccagaatgg
     7501 ccacggcgat cccccgcgtc cgcagggccg tttccagcgc ggcggcgtcc tgcaccacca
     7561 ggtcgccaat ggtcttgccg atcttggccg ggtcgttgtc gaagacgcag gtgatgacga
     7621 agccgcgccg ctggaagcca cggtaactgg ccagcgcatg ccccaggaag cccgcaccga
     7681 cgagggccac cggccactca tgggtcaggt tcaggatttc ggtcagccgg tcgatgaggt
     7741 agtggatgtg atagccggtg ccctgcttgc caaattcgcc aaagtgggag aagtccttgc
     7801 gaatctgggc cgaactcatg cccagccgct cggccaactc gtgcgaggac gtggttgcct
     7861 tgccggcgtc gcgcaccatg cgctccaact cgcgcaaata gacgggcagg cggccgataa
     7921 cgatgtcggg gatttgttcg tccacgatcc ctttcccgaa tgctaatcgt gcattctttc
     7981 acgaacattt tagcagaggc tttgtggctg gcaaatgccg ggtcaccagt gcccagcgcg
     8041 ggcgcgccac cgggccgtct ggcgtgttgg ccgcgtttgt ctctaccgaa atgaccgctt
     8101 agccgccggc gtgggtatgg gatgtcattg tttgaatact catgagaggc aagtgcccct
     8161 tcgccggttt ctgactataa ttctctccta ggctgggcaa cttgcccgag caaccaactg
     8221 ggtaacagat gattagccct gaccggcggt cgggagccaa gcttcatccg tcttggaaca
     8281 atctatgtca cgcactttca ggaacatcct ccttgtcgtc ctggcgctgg ctgtcgtcgg
     8341 cgcgggcgtc ttctggttcg tcaaccgccg ccaggccgcg gccgcggcgc aggtcgaaat
     8401 cctgcgcgag gccaccgtcg cccgcgacac catcacctcg accgtcaacg ccaccggcgc
     8461 catcgagccg gaaacgctgg tgacgctgac ctttggcctc agcggcacag tcaccgatgt
     8521 ggccgtgacg cgcgggcagg tcgtggccgc cggcgacgtg ctggcccaac tcgacaccag
     8581 cgagccggcc ctggcggtgc agcaggccga ggacgcgctg cgcatccagg agctaaccct
     8641 ggcccagcgc gtcgagggcg gcccaagtcc ggcccaactg gcctcgtcgg cggccgacat
     8701 ccaggccgcc gaggccaacg tccaggtcgc ccaggccaac ctcagtggcg cgcaggccgg
     8761 cgtgcagcag gcccaggcgc agcgggcgca actgctggag ggggccacgc ccggccagat
     8821 cgccgcggcc gagtcgcagg tggccgccgc ccagcagcag cagaaacagg ccgaggagac
     8881 ccacaacaag accctggagt gcgtcacctt cgccgtgccg ggcacgaacc agcaagacga
     8941 ggtctgcccc ggcctgggcg agccggagga agccgcccgc gccgcgctgg agacggcgac
     9001 gctgaacttg caggccgccc aggcgcaact ggccgacctg cagaacccgg ccagcgcggc
     9061 caacatcgcc gctgccgacg ccgccgtggc cgccgccgct gcccaggtgg aggccgccga
     9121 gggcaacgtc gccgccgccg aggcgcaact ggcccgcgcc cgcgcggcca acgacctgtt
     9181 gcgcgagggg ccgtcggccg cagaacaggc cattctcgac gcccaggtgg ccagcgcgcg
     9241 caccaacctg gagattgccc aactgcgcct taagcaggcc cagctcatcg ctcccatcga
     9301 cggccaggtg gccagcgtcc tcatccgcgc gggggagcag gccgcgcccg gcgcgccggc
     9361 catgaccgtc ctcaacgaga acgcctacca catcactgtc caggtcgatg agatcgacat
     9421 cgaccgcgtc gaggccggcc agccggcgct gattaccctc gacgccctgc ccgataaacc
     9481 ggtgcagggc acggtca
//
LOCUS       DAOSMQ010000060         9487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7182_length_9487_cov_5.297392, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000060 DAOSMQ010000000
VERSION     DAOSMQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7182_length_9487_cov_5.297392"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..2693)
                     /locus_tag="PK829_03315"
     CDS             complement(144..2693)
                     /locus_tag="PK829_03315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU40277.1"
                     /translation="MVSRFASLSAVSGWRPAGRGRSSVVLLLALLLLAFGLRAHRIGD
                     QRVWWDEGWSVWAARFPVAGILRETGNDVHPPLYFTLLHVWRAGSGDSEAGLRLLSAF
                     LGLLTVAATYALGRSMARGLLPPGAARAVGLLAALLLTVSRFANAWSQEIRMYALATL
                     LGVLAVWAARRVWAGGGRRAAAVYVLATTAGLYTLYLFAPIWVAINVAWVVGLWPRRK
                     NPEWIALQLLIAALFLPWAVYAAGGFLSTASATPIRLLDFLHIYWTVLTVGIPVDVAQ
                     FNWLTLPALAIFVVAVTALVWTVGKKLTQGRQEAKTQRAETAGIGGRRVLADVVVLLA
                     VLLLPIVLVYFISLPRQNFYNPPFNPRYLVIFTPFYSVVLAWGLMMLGLWIGSLRRGG
                     TESEGAGKRGSRGEKKTRRTAERLTYRPATGHWPLATILLAVFMLAVALVGLWPYYPG
                     RIRIDDYPSLVSTLAAQRRPGDSVVLYTDTDWPIFAFHHPQPWRGVPHLWAMTPELAE
                     DFLAPIWAGHDAVWLVTTPYSAAGDPQRLVPAWLAARATAVRAFPYGDMALTLYTRTA
                     ARAAAAGAPAPDAPPPRPLAIPLPTGAITGYTQATRDFRSGDTVRLFLFYSATAGSDI
                     TVALLDATGAAWGATAVTLPAAPEWGRQQIDLLVPPDAPSGDYRFAAAGAGTNGAGAA
                     TPFGRLTIRQKDAARLTEADVTISQRLDVPFAGGVRLLGYDLAAEQVRPGEAVELTLY
                     WSAAGPLAPAHKVFTHLLGETFNATSGNFLWGQSDAEPAANTRPTTTWRAGEVIVDSH
                     LIPVAADAPAGRYTLEVGLYEPTSGARLAVVGAAGAAVDDHVVVTTVVVEP"
     gene            complement(2791..4503)
                     /locus_tag="PK829_03320"
     CDS             complement(2791..4503)
                     /locus_tag="PK829_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU40278.1"
                     /translation="MSETIFGRLATLEVRNEFAQLRLKGLQHRRRLSPGSPRAGEQPV
                     VTVTVGPDLPVDEVVCLVTEPQAAVVPLRRVSTDFDVPLWAYIETWEAALPAQPAGTL
                     VRYQLRAHDEDSNEIYWVDEGAEYSYVVGDSGPPAWALPASIYQVFPDRFSPGAGRAW
                     NPAARLSDIYGGTLRGIIDNLGYIANLGFTAIWLNPFFPDDTHHGYHATDYFAVNPRL
                     GTLDDARELVDKCHERGIHLLLDFVGNHWGSRHSTFQAALADRNSPYYSWYYWKNWPD
                     QYLAYYDVPDLPKLNVDHPGVRDHLLRSVGFWLGDVGFDGLRLDHADGPSYDFWTDVR
                     VVARSIKPDAWMFGELVRTPETQLSYAGLFDGTLDFLLCQAMRNTFATGEMSLAAFDA
                     FLNRHEAYFPAQNVFSRPSFLDNHDMDRFLWRAGNDKRRLKLAALAQFTLGGAPIVYN
                     GTEVGVSQTCNIHDPDSQGMEECRQPMPWGDEQDGELHAYFWYLSHLRRNHSALWRGG
                     RQTLLLDSAAQTYAYAREDGADALIVALNMSEEPRTVVVPHVERGAQVTFELPPWTGD
                     VAAV"
     gene            complement(4512..4802)
                     /locus_tag="PK829_03325"
     CDS             complement(4512..4802)
                     /locus_tag="PK829_03325"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HOU40279.1"
                     /translation="MTDESIVYFSEYSEFDEEGELSVKTYVYHWMTASKNLRRRWDNT
                     PHYPHLPESLNHVHDGDEKNVLSSAPMNLLKVLDIIADELKKTTGGRPQTAS"
     gene            complement(4927..5265)
                     /locus_tag="PK829_03330"
     CDS             complement(4927..5265)
                     /locus_tag="PK829_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40280.1"
                     /translation="MTAQMPDIRSSKRASRLLPLIRLYESGQASERMDRTLDKLLSTE
                     EAETKDTIEQLRADLDQFEQQYGMTSAAFYERFAEGELGDDMEYVTWASLFQMYTRAL
                     NRLHILTEAK"
     gene            complement(5361..8615)
                     /locus_tag="PK829_03335"
     CDS             complement(5361..8615)
                     /locus_tag="PK829_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HOU40281.1"
                     /translation="MRRIIVLILSLLIMAACRQEGNATPTAAPEALGETATPATAPAP
                     TEPPPTPAPTEEPPALPTAAPTAVPAACNPDDPIYLAIVWHQHQPVYFQDPETGVFSR
                     PWVRLHAAKDYVDMATMLRDYPDVKVTFNLTPSLIRQLEALSAGATDLAQEVTLVPAG
                     ELTDEQKQFILDRFFDTNRKVIARFPRYQELLNLRDGSDAPLTAYTTDDFRDLQVLFN
                     LAWTDPDWLAQEPLAALVDKGEGFSEADKQVILDEHLRLVNEVIPVHRELQDSGQIEV
                     TTTPFAHPILPLLVDTDMALIALPEATLPNPPFRFGQDAVAQVQLGVDFYTERFGQPP
                     RGMWPSEGAVAEGMISMVNAAGIQWMASDESVLARSIGLDSFARDGNEVVVEADRLYR
                     VYNVQGANGGPVAMIFRDQILSDKVGFGYSGLSAEAAVDDFVRRINAICGRLQTQGGQ
                     GPHLVSVILDGENAWENYDNDGKEFLHALYGRLSEETAIVTTTPSAYLAMTQERERIG
                     NLWAGSWHFADYSIWIGEDEENRAWDLLRDTRTYLQTYVTGRNRDNATPEEIDAAFTQ
                     MYIAEGSDWFWYLGADQSSADDASFDAQFRNTLKRVYTELDAEPPAILDVPIIPAQTA
                     EADRLASGTITPLIDGVADAGEWDAAGSYLATGGVMATTPVFAELAYGFDARNLYLKT
                     TINPDFTMPASTSIEYYLSSPAGGPVSNFSRSGELLGFAANRMLELKFGGGILGGVTF
                     YQAAAEGASPDGWSVVASGTARNGQAAFDVSDEAAGGVAAGEVIEMAIPLAALGSPDV
                     GARLFVRGVYIEPVEGERAEVDRIPSLGPGELAVPDLGETTALLDVTDPTGDDTGPGT
                     YTYPTDVVFQPGNFDITNFQVGADEDNVVFRFTMAGPVDNPWDGPNGLSLQTFDIYID
                     QDGDGAGGVAFLPGRNLALAEGSAWDYAITVEGWESKIFTPGDNAPTEIAGPGDFQVI
                     TDPGQQKVTIRVPKSILGDAPEAWRFAAVVMSQEGFPSPGVLRVRDVAPTAEQWRIGG
                     APAGATNATRVLDLVWPDAGQQETWLSDFTPAAAAQGSLTAADFARVEMLTPGP"
     gene            complement(8918..>9487)
                     /locus_tag="PK829_03340"
     CDS             complement(8918..>9487)
                     /locus_tag="PK829_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU40282.1"
                     /translation="FSRFRWRGRSQLLLVILLIQVFPAILAMVALFAILQQIGNYIPA
                     LGLNSHGGLILIYMGGTLGVNVWLMKGFYDSIPREIDESGKVDGASDWQIFWRLLFPL
                     VRPIMVVSMILTFFGTYSDYLMPRIMITSASKFTLMLGLQTFIGSNYAQEWGAFAAGA
                     VIGAIPMVTIYLLLQDYIVGGLTAGSVKG"
BASE COUNT         1555 a         3069 c         3324 g         1539 t
ORIGIN      
        1 ttgtcaacct gcctcttcgc gccccaggat gcaggataac atcctgcgct acaactcgtc
       61 gccgttgtag cgcaggttgt caacctgcct cttcgcgccc caggatgcag gataacatcc
      121 tgcgctacaa cccggcgcga gaactacggc tcgaccacga ctgtcgtcac caccacatga
      181 tcgtccacgg ccgccccggc cgcgcccacg acggccagcc gcgccccgct cgttggctcg
      241 tacagcccca cctccagcgt atagcggccc gcgggcgcat cggcggcgac ggggatgagg
      301 tgactatcga cgatgacctc gcccgcgcgc caggtggtcg tcggccgcgt gttggccgcc
      361 ggctcggcgt cgctctggcc ccacaggaag ttgccgctcg tggcgttgaa tgtctcgccc
      421 agcaggtggg tgaagacctt gtgcgccggg gccagcgggc cggcggccga ccagtacagc
      481 gtcagctcga ccgcctcgcc cggccgcacc tgttccgcgg ccaggtcgta gcccagcagc
      541 cgcacgccgc cggcaaaggg cacgtccagc cgctggctga tggtcacgtc ggcctcggtc
      601 agccgcgcgg cgtctttctg gcggatggtc aggcggccga agggcgtggc cgcgcccgcg
      661 ccattcgtcc cggccccggc ggcggcgaag cggtagtcgc cgctgggcgc gtccggcggc
      721 acgagcaggt cgatctgctg ccgcccccat tccggcgcgg ccggcagcgt caccgccgtc
      781 gcgccccagg cggcgccagt ggcgtccagc agggccacgg tgatgtcgct gccggccgtg
      841 gccgaataga agaggaacag gcgcacggtg tcgccgctgc ggaagtcgcg cgtggcctgg
      901 gtgtagccgg tgatcgcgcc cgtgggcagg gggatggcca gcgggcgggg cggtggggcg
      961 tcgggggcgg gcgcgccggc cgccgcggcg cgcgccgccg tgcgcgtgta gagcgtcagg
     1021 gccatgtcgc cgtaggggaa ggcgcgcacg gccgtggccc gcgccgccag ccaggcgggc
     1081 acgagccgct gcgggtcgcc cgccgcgctg tagggcgtcg tcaccagcca gacggcgtca
     1141 tggccggccc agatgggcgc gaggaagtcc tcggccagtt ccggcgtcat ggcccacagg
     1201 tggggcacgc cgcgccacgg ctgcgggtgg tggaaggcga agatgggcca gtcggtgtcg
     1261 gtgtagagga cgacgctgtc gcccggccgc cgctgggccg ccagcgtgct gaccagcgac
     1321 gggtagtcgt cgatgcggat ccgccccgga tagtagggcc acagcccgac aagggccacg
     1381 gcgagcatga atacggccag gaggatagtg gccagtggcc agtggccggt ggccgggcgg
     1441 tacgtcagcc tttcggccgt gcgtcgcgtc ttcttctctc ccctgctccc ccgcttccct
     1501 gctccctcgc tctctgtccc tccccggcgc aacgagccga tccacagccc caacatcatc
     1561 agcccccacg ccaacaccac gctatagaac ggcgtaaaga tgaccagata gcgcgggttg
     1621 aagggcgggt tgtagaagtt ttgccgcggc agggagatga agtagaccag cacgatgggc
     1681 aacagcagca cggccagcag aacgacgaca tcggccagca cccgtcgtcc gccaatccca
     1741 gctgtttctg ccctttgcgt ctttgcctct tggcgtcctt gcgttaactt cttcccaacc
     1801 gtccacacta aggctgtgac ggcgacaacg aagatcgcca gcgccggcag cgtcagccag
     1861 ttgaactggg ctacgtcgac gggaatgccg acggtcagca ccgtccagta gatgtggagg
     1921 aagtcgagca ggcggatggg cgtggccgag gccgtgctca ggaagccgcc ggcggcatag
     1981 acggcccagg ggaggaagag ggcggcgatg aggagttgga gggcgatcca ctcggggttc
     2041 ttccggcgcg gccaaagccc caccacccag gccacattga tcgctaccca gatgggcgcg
     2101 aacaggtaga gggtgtagag gccggccgtc gtggccagga cgtagacggc cgccgcccgc
     2161 cgcccgcccc cggcccacac ccgccgcgcc gcccagacgg ccagcacgcc cagcagcgtc
     2221 gccagggcgt acatgcggat ctcctgcgac caggcgttgg cgaagcgcga gacagtcagc
     2281 agcagcgccg ccagcagccc gacggcccgc gccgcccccg gcggcagcag cccgcgggcc
     2341 atgctccggc ccagggcgta ggtagcggcg acggtcagca ggccgagaaa tgccgacagc
     2401 agccgcaggc ccgcctcgct gtcgccgctg cccgcccgcc agacgtggag cagggtgaag
     2461 tacagcggcg ggtggacatc gttgcccgtc tcgcgcagga tgccggccac gggaaagcgc
     2521 gccgcccaga ccgaccagcc ctcgtcccac cagacgcgct ggtcgccgat acggtgggcg
     2581 cgcaggccga aggccagcag aaggagagcg agcaggagga cgacggacga ccgaccgcgg
     2641 ccggccggcc gccagccact gaccgcagac agagaagcga atcggctgac catagtgggc
     2701 gttcggccgg tgtcaacaga gcgacgcacc tccgccggct cgccgtcggc ggtcggttgg
     2761 gcggtcggcc gtcggccgtc ggcggtcatg tcagacggcg gccacgtcgc ccgtccacgg
     2821 cggcagctcg aaggtcactt gcgcgccgcg ctccacgtgg ggcacgacga cggtgcgcgg
     2881 ctcctcgctc atgttcaggg cgacgatgag cgcgtcggcg ccgtcctcgc gagcataggc
     2941 gtaggtctgc gcggccgagt cgagcagcag cgtctgccgg ccgccgcgcc acagcgccga
     3001 atggttgcgg cgcaggtggc tgaggtacca gaaataggcg tgcagttcgc cgtcctgctc
     3061 atcgccccag ggcatcggct ggcggcactc ctccatgccc tggctgtccg ggtcgtggat
     3121 gttgcacgtc tgcgacacgc cgacctcggt gccattgtag acgatgggcg cgccgccgag
     3181 ggtgaactgc gccagcgcgg ccagcttcag ccgtcgtttg tcgttgcccg cccgccacag
     3241 gaatcggtcc atgtcgtggt tgtccaggaa gctgggccgg ctgaagacgt tttgcgccgg
     3301 gaagtaggcc tcgtgccggt tcaggaaggc gtcgaaggcg gccaggctca tctcgcccgt
     3361 ggcgaaggtg ttgcgcatcg cctggcagag caggaagtcg agcgtgccgt cgaagaggcc
     3421 ggcgtagctg agttgtgtct cgggcgtgcg cacgagttcg ccgaacatcc acgcgtccgg
     3481 cttgatgctg cgggcgacga cgcgcacgtc ggtccagaag tcataggatg gcccgtcggc
     3541 gtggtcgagg cgcaggccgt cgaagcccac gtcgcccagc cagaagccga cactacgcag
     3601 cagatggtcg cgcacgccgg ggtgatcgac gttgagcttg ggcaggtcgg gcacgtcgta
     3661 gtaagccagg tactggtcgg gccagttctt ccagtagtac caactgtagt aggggctgtt
     3721 gcggtcggcc agcgcggcct ggaaggtgct atgccggctg ccccagtggt tgccgacgaa
     3781 atcgagcagc aggtggatgc cgcgctcgtg gcacttatcg accagttcgc gggcgtcgtc
     3841 gagcgtgccc aggcgcgggt tgacggcgaa gtaatccgtg gcgtggtagc cgtggtgggt
     3901 gtcgtcgggg aaaaagggat tgagccagat ggccgtgaag cccaggttgg cgatgtagcc
     3961 caggttgtcg atgatgccgc gcagcgtgcc gccatagatg tcggacaggc gcgccgccgg
     4021 gttccaggcg cggccagcgc ccggcgagaa gcggtcgggg aacacctggt agatgctggc
     4081 cggcagcgcc caggccggcg ggccggaatc gccgacgacg tagctgtact ccgcgccttc
     4141 gtccacccag tagatctcgt tgctgtcctc gtcgtgggcg cgaagctggt agcgcaccag
     4201 cgtgcccgcc ggctgggcgg gcagggccgc ctcccacgtc tcgatgtagg cccacagggg
     4261 cacgtcgaag tcagtgctga cccgccgcag cgggacgacg gccgcctgcg gctcagtgac
     4321 caggcagaca acttcgtcaa cgggcagatc aggccccacg gtaacggtga cgaccggttg
     4381 ttcgccggcg cgcggcgaac cgggcgacag ccggcggcgg tgttgcagcc ccttcaggcg
     4441 cagttgggcg aattcattgc ggacttccag agtggccagg cggccgaaga tggtctcgct
     4501 catagttccc cttaggaggc ggtctggggc cggccgccgg ttgttttctt gagttcgtcg
     4561 gcgatgatgt cgagcacttt gagcaggttc ataggcgcgc tggagagaac gttcttctcg
     4621 tcgccgtcgt ggacgtgatt tagagactcg ggcaaatgag gatagtgcgg agtattgtcc
     4681 cagcgccgcc tcaggttctt gctcgccgtc atccaatgat aaacataggt cttgacgctc
     4741 aactcaccct cttcgtcgaa ctcgctgtat tcggagaaat agacaatgct ctcgtccgtc
     4801 aggtagatac gcgctcggac aaaagcagag tcatctgtcg cgcgctcaaa caggaattca
     4861 tacgctgcta cccattgatg atcgatcagg tgactcttga tgctatcgat ataccacggg
     4921 gcgtgatcac ttcgcctcag tcaagatatg cagccgattc aacgcgcgcg tatacatctg
     4981 gaaaagtgag gcccacgtca cgtattccat atcgtcgcct aactcgcctt cggcaaatct
     5041 ttcataaaaa gcggccgacg tcatgccata ttgctgctcg aattggtcta gatcggcgcg
     5101 caactgctca atcgtgtctt tcgtctccgc ttcctccgtt gagagcagct tgtcgagcgt
     5161 cctgtccatt cgctcgctcg cctgaccgct ttcataaagg cgaatcagcg gtagcaggcg
     5221 cgacgcgcgt ttgcttgagc gaatatcggg catctgtgcc gtcatcatgc acctccgcgt
     5281 gaagtggttg ccagttacca gtggctgcac cgccgatcac tggccaccca ttataccgaa
     5341 gacgcctgtg cccggcagac ctacggcccc ggcgtgagca tctccacccg cgcgaagtcc
     5401 gcggccgtca ggctgccctg ggcggccgcg gcgggggtga agtcgctcag ccacgtctcc
     5461 tgttgccccg cgtccggcca gaccaagtcc agcacgcgcg tggcgttggt ggctccggct
     5521 ggcgcgccac cgatgcgcca ctgttcggcg gtgggagcca cgtcgcgcac gcgcagcacg
     5581 ccgggggagg ggaagccctc ctggctcatg accacggcgg cgaagcgcca cgcttccggc
     5641 gcgtcgccca ggatgctctt gggcacgcgg atggtgacct tctgctgacc agggtcggtg
     5701 ataacctgaa agtcgcccgg cccggcgatc tccgtcggcg cgttgtcgcc gggtgtgaag
     5761 atcttcgact cccagccctc gacagtgatg gcgtagtccc aggcgctgcc ctcggccagg
     5821 gccaggttgc gccccggcag gaaggccacg ccacccgcgc cgtcgccgtc ctggtcgatg
     5881 tagatgtcga acgtctgtag cgacaggccg ttgggcccgt cccagggatt atcgaccggg
     5941 ccagccattg taaagcggaa gacgacgttg tcctcgtccg cgccgacctg gaagttggtg
     6001 atgtcgaagt tgcccggctg gaagaccacg tcggtcgggt aggtgtaggt gcccgggccg
     6061 gtgtcgtccc cggtggggtc ggtcacgtcc agcagggcgg tagtctcgcc cagatcgggc
     6121 acggccaact cgccggggcc gagcgagggg atgcgatcca cctcggcccg ttcgccctcg
     6181 accggctcga tgtagacgcc gcgcacgaag agccgcgcgc caacgtcggg gctgcccagc
     6241 gccgccaggg ggatggccat ctcgatgacc tcgcccgcgg ccacgccacc ggcggcctcg
     6301 tcggacacgt cgaaggcggc ctggccgttg cgggccgtgc cgctggcgac gacgctccag
     6361 ccgtcaggcg aggccccctc ggccgcggcc tgatagaagg ttaccccacc cagaatgccg
     6421 ccgccgaact tcagctccag catccggttg gcggcaaagc ccagcagctc gccactgcgg
     6481 ctgaagttgc tgaccggccc gccggcgggc gaactgagat agtactcgat gctcgtcgag
     6541 gccggcatgg tgaagtcggg gttgatcgtc gtcttcaggt agaggttgcg ggcgtcgaag
     6601 ccgtaggcca gctcggcgaa aaccggcgtc gtagccatga cgccgcccgt ggccaggtag
     6661 ctgccggccg cgtcccactc gcccgcgtcg gccacgccat cgatgagcgg cgtgatcgtg
     6721 ccgctggcca gccggtcggc ctcggccgtc tgtgccggga tgatgggcac gtccaggatg
     6781 gccggcggct cggcgtccag ctcggtgtag acgcgcttga gcgtattgcg gaactgggcg
     6841 tcgaaggagg cgtcatcggc cgacgactgg tcggcgccga gataccagaa ccagtcgctg
     6901 ccctcggcga tgtacatctg cgtgaaggca gcatcgatct cctccggcgt ggcgttgtcg
     6961 cggttgcggc cggtgacgta ggtttgcagg taggtgcgcg tgtcgcgcag caagtcccag
     7021 gcgcggttct cctcgtcctc gccgatccag atgctatagt cggcgaagtg ccacgagcca
     7081 gcccacaggt tgccgatgcg ctcgcgctcc tgggtcatgg ccagatacgc gctgggggtc
     7141 gtggtgacga tggccgtctc ctcgctcaga cggccataga gggcgtgcag gaactccttg
     7201 ccgtcattgt cgtagttctc ccacgcgttc tcgccgtcga ggatgaccga gacgagatgc
     7261 ggcccctggc cgccctgggt ctgaaggcgg ccacagatgg cgttgatgcg ccggacgaag
     7321 tcatcgacgg cggcctcggc tgagaggccg gagtagccga agccgacctt gtcggacagg
     7381 atttggtcgc ggaagatcat cgccaccgga ccgccgttcg cgccctggac gttgtagacc
     7441 cgatagagcc ggtcggcctc gacgacgacc tcgttgccgt cgcgggcgaa gctgtccagg
     7501 ccgatgctgc gggccagcac gctctcgtcg ctggccatcc actggatgcc ggcggcgttg
     7561 accatgctga tcatgccctc agccacggct ccctccgaag gccacatccc tcgcggcggc
     7621 tggccgaagc gctccgtgta gaagtccacg cccagctgca cctgggccac ggcgtcctgg
     7681 ccgaagcgga acggcgggtt gggcagcgtc gcctccggca gggcgatgag ggccatgtcg
     7741 gtatcgacca gcagcggcag gatggggtgg gcgaaaggcg tcgtcgtcac ctcgatctgg
     7801 ccgctgtcct gcaactcgcg gtggacgggg atgacctcgt tgaccagccg cagatgctcg
     7861 tcgagaatga cctgcttgtc ggcctcgctg aagccctcgc ccttgtccac cagcgccgcc
     7921 agcggctcct gggccagcca atcggggtcg gtccaggcca ggttgaagag cacctgcaag
     7981 tcgcggaagt cgtcggtggt gtaggcggtc agcggcgcgt cgctgccgtc gcgcaggttg
     8041 agcaactcct ggtagcgcgg gaagcgggcg atgaccttgc ggttggtgtc gaagaaacgg
     8101 tcgaggataa actgcttctg ctcgtcggtc agctctccgg ccgggacaag ggtcacttcc
     8161 tgagccaggt cggtcgcccc ggcgctcagc gcttccagtt ggcggatgag gctgggggtc
     8221 aggttgaaag tcaccttgac gtcggggtag tctcgcagca tggtggccat atcgacgtag
     8281 tctttggccg cgtgcaggcg cacccagggg cggctgaaga cgcccgtctc cgggtcctgg
     8341 aagtagacgg gttggtgctg gtgccagacg atggccagat agatgggatc gtcggggttg
     8401 caggcggcgg gcacggctgt cggcgcggcc gtgggcaggg ccggcggctc ctccgtcggc
     8461 gcgggggtgg gcggcggctc agtgggcgcg ggagcggtgg ccggcgtggc cgtctcgccg
     8521 agcgcttccg gggcggctgt tggtgtggcg ttgccctcct gccggcaggc ggccatgata
     8581 agcaagctca gaatgagaac aatgatacgg cgcatgatta cctctcattg cgaataatat
     8641 gctcatagtc gtggcgttgc caaaagggct gaccttgaat accttggctc tggttgatac
     8701 gtcgggcggg aaacgacttg aaggcgcgaa caatttcggg taagccgtga tgatggccac
     8761 tgtagataga ggcaggttca agccctgtct cggccggggg caatggggca gtagaggcat
     8821 cggtgttggt gtaggggcgg gtctcagacc cgcccctaca ccgtggcgac acctcatgta
     8881 ggtagggcgg gtctaaggac ctgcccctac cggcgaacta gcccttaacc gagccggctg
     8941 tcaggccacc gacaatgtag tcctgcaaga gcagatagat ggtgaccatc gggatggccc
     9001 cgatgaccgc gccggcggcg aacgcgcccc actcctgcgc gtagttcgag ccaataaacg
     9061 tctgcaagcc gagcattagg gtgaacttcg acgccgacgt gatcatgatg cgcggcatca
     9121 ggtagtcgct gtaggtgccg aagaaggtca ggatcatcga cacgaccatg atcggccgca
     9181 ccagcgggaa caacagccgc cagaagatct gccagtccga cgcgccgtcc accttgcccg
     9241 actcatcgat ctcgcgcggg atggagtcgt agaagccctt catcagccag acgttgacgc
     9301 ccagcgtgcc gcccatgtag atgaggatca ggccaccgtg gctattcagg cccagggcgg
     9361 gaatgtagtt gccgatctgc tgcaagatag cgaacagggc gaccatggcc aggatggccg
     9421 ggaagacctg aatgagcagg atgaccagca gcaactggct gcgcccgcgc cagcggaagc
     9481 gcgagaa
//
LOCUS       DAOSMQ010000061         9421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7249_length_9421_cov_4.782084, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000061 DAOSMQ010000000
VERSION     DAOSMQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7249_length_9421_cov_4.782084"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PK829_03345"
     CDS             <1..1421
                     /locus_tag="PK829_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40283.1"
                     /translation="LNDLANAFDITFRGDYLYNTVENEGNFRNILIGADGFGDSALTE
                     GLDRLALYSSHSLQLGPSAAALLSADDNTWSSATDRPGGLVLAALSPAASDAGQLLAI
                     GDVHFLTEPYSTVYDNGAFVARVADFLAGGGSTGTLAGFPYFFHSPVDLVYSDDPDLG
                     PDAFDDIIALQAAFRGVGLSLALANEAAADHDVIYAGLYNTAEDVAELLDAAGVSLAI
                     RPAIARPATTATTDPDATPTPSHGTRLIESPLGDVEMAGTALLLLADDGTRRQLVVLA
                     ASNDGLENALTRLRGAASAEGVNLTDCLIQDNLALCPTGVANEPVEYALVTSGPADEP
                     TTESEQPNGSQADSPEPTDKGAIELGQTKTAELAAGEIHAWTFRDGPATIDITVDPDA
                     DMDAVLELYAPDGTLMANLDSAFAGGVEEMRAVEIPDDRPYFIIVRDFFNNGGHYQLS
                     VAAGEPAQPDQPEPTPTPEPGG"
     gene            1428..3044
                     /locus_tag="PK829_03350"
     CDS             1428..3044
                     /locus_tag="PK829_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOU40284.1"
                     /translation="MNSPDILLIVLDTQRADRLGCYGHDRPITPNLDGFAGGGALFER
                     AVSPAQWTIPSHASLFTGLYPTAHGVTQSSQSLSSGRPHLAQVLRQLGYETVGFCNNP
                     LVGVLNNGFKRGFGAFYNYGGAFPSLPRQSNPWPWPLNRVAEGYTQFLRRISYPIQNF
                     FGQSDLAFRVSLHAFLTPLWSKMANFKGQNERSVDDVVQFLEERETKPADRPLFLFLN
                     LMETHLPFWPPGEFIDRVAPYFRESKAARTILRTWNREAYRWAAPLAEPLDELESRVL
                     NDLYDAEVAYQDDYLGRLFAALAARGRSRDTLTIIVADHGDGLGDHGYFGHAFVAYDE
                     LVHVPLIAHWPARLAAGARVATPVSTRRVFHTLIDAAAPTPETAAVVAEVLAEDAPNV
                     RRLSLRHTLRGRDPEGATAFSEVYPPLNFVRAIETRQPELLTQFRCLATRRAVVRPMR
                     ATSGAAAPLKLIHVDGTADALYDLAADPREQDDLLTQQPEIAAALDLALEQMAQRMER
                     EREAQPAGAAVDLDSDELLRQRLRGLGYLE"
     gene            3073..3960
                     /gene="lgt"
                     /locus_tag="PK829_03355"
     CDS             3073..3960
                     /gene="lgt"
                     /locus_tag="PK829_03355"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU40285.1"
                     /translation="MLDGIQIDPVAFTIPIGDGFPIYWYGILVTIGIAIGVWWGAREI
                     ARRNQNVDEFFNGLILVIFSGYAFARLTYVTMESLAGRGAQFETFWDLINIRSGGINI
                     LGGFIGAAVVGFIYVRARGLNFWQYADVAGPAVLIAQAIGRWGNFINQELYGPPTTRP
                     WGILIDADHRLPQYADLTQFPLTTRFHPTFLYESIWLLLGFIVLVALNNRNRDKWRAG
                     TLFGLFLIWWGAGRAVLEFFRPDQVTVGDSPITYSFLLALGLAIAGIWVVLRRNNRRP
                     EVFTRRRRRQLKPKPRREA"
     gene            4005..5513
                     /gene="guaB"
                     /locus_tag="PK829_03360"
     CDS             4005..5513
                     /gene="guaB"
                     /locus_tag="PK829_03360"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOU40286.1"
                     /translation="MQFANEYGETALTFDDVLLAPGYSEVLPAQTHTRTRLVGDLYLS
                     IPILSAAMDTVTEAPMAIALARLGGLGVIHRNLTPEEQAEEVDKVKRSESGMIVDPLT
                     LRPDDTLATAEALMARYHISGIPITDGDGRLVGILTNRDTRFVQPGAQPIADFMTREG
                     LVTAPVGTSLEEAKAILHRRRIEKLPLVDGDGRLKGLITVKDILKKIDYPDSAVDARG
                     QLLCAAAIGVGDKALARLDCLVEAGVDAAAIDTAHGHTRLVLETIRAARRRHPRLPIL
                     AGNVIDGAGARALIEAGAGAIKVGIGAGSICTTRIVAGAGVPQVSAIAAVAAVARALD
                     TPVIADGGIKYSGDIVKALAAGADAVMLGSLLAGLEESPGEIILYEGRRYKVYRGMGS
                     MGAMQGFGADRYGSGVAAADGLHTPAGGQVRDKLVPEGIEGQVPYKGRLGDVVYQMMG
                     GVRSGMGYVGAADLAELRAKARFVRITNAGLLESHPHGVLITKEAPNYQARR"
     gene            5537..6424
                     /gene="rarD"
                     /locus_tag="PK829_03365"
     CDS             5537..6424
                     /gene="rarD"
                     /locus_tag="PK829_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOU40287.1"
                     /translation="MNKGAVYAAGAYILWGLLPIYWKTLHSVPSTQIVAHRLVWSLGF
                     VALVLTARHNWGWLGGVRRRPRVLLTFALSGALLTANWMVYIWGVNAGFIVETSLGYF
                     INPLVNVMLGYVILKERLRPPQWAALAIALGGVLYLTFSYGAFPWIALTLAFSFGFYG
                     LIRKTAALNSAEGLFLETALLFLPALGFLVLQEVRGVGALAHTDARTTILLIGAGAVT
                     SIPLILFAAGARRITLTTLGLLQYIAPTLQFLIGVLIYHEPFGAGRVVGFGLIWLALI
                     LYTTESLAHYRRIAAPVQT"
     gene            6469..6774
                     /locus_tag="PK829_03370"
     CDS             6469..6774
                     /locus_tag="PK829_03370"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOU40288.1"
                     /translation="MVTIVSIDLISTDQTVRGGKACIAGTTLRVIDVVMASIFHGRTP
                     SAIASDFDVSLAQVHAALAYYYQHKTQLDEDIRAQLDRAQAFKQERLGSQHASLLSG"
     gene            6740..7078
                     /locus_tag="PK829_03375"
     CDS             6740..7078
                     /locus_tag="PK829_03375"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOU40289.1"
                     /translation="MAANTLRYYLDENLPVEIARQLRLRGIDAVTVRDLGRLGEPDSN
                     HLHRATDMGMVLCTFDTDFIELVAAGQQHAGIVLGQQDVHEIGAWVRYLELMHTVYSA
                     EDMQDHIEYL"
     gene            7094..8509
                     /gene="purB"
                     /locus_tag="PK829_03380"
     CDS             7094..8509
                     /gene="purB"
                     /locus_tag="PK829_03380"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU40290.1"
                     /translation="MPTTDHQTYASPFTWRYGSEPMRYLWSETHKRRLMRQVWVALAA
                     AQHVAGLVTAEQLADLEAHVDSVDIPRALQIERETRHDVVAEIRAFAEQCRVGGGIIH
                     WGATSADITDNVDVLRQREAARLLAEQLRRLLLAFARRIEETADVVIMAQTHMQPAEP
                     TTLGYRLAMYAQDLAEDESRLNGLISDLRGKGLKGAVGTQAGFIEVLDVCDMTVVQLE
                     ELAMARLDLPYFPVAGQTYTRQQDLRVQSVLAGIAAALHKFALDCRILQSPPFGEWSE
                     PFGQRQVGSSAMPFKRNPINAENICSLARFVAAQTAVAWDNAAQAVLERSLDDSANRR
                     LFLPESFLAMDEMLRRATALVEGLIFDHEQMARNVTRYGPFAATERVLMAAVRAGADR
                     QAAHEWLREASLRAWEAIRHDQANPLTELLSADARLTAYLPAETVSALMDAGGYVGTA
                     AERARALADTLKTPLPNPRRL"
     gene            complement(8740..>9421)
                     /locus_tag="PK829_03385"
     CDS             complement(8740..>9421)
                     /locus_tag="PK829_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40291.1"
                     /translation="LSGRGAGVLPVVAAEPLATQWRTLWPGADEATVARVYTDLVSRY
                     ADPQRAYHTLGHITDCLRHFDAVRHLAAWPAAVELAIWFHDAIYDPQRGDNETRSAEL
                     AEVTLRALAGDRLAAATQAPTGSGLPAAVGRLIRLTSHAHAQPPATERCWVISTWPSW
                     EQHRRCLMPSTACSTACQAVCSTACQAVAAAGDDSLTGCATLRSMRPVRLSVAQPVRL
                     WLRPATTA"
BASE COUNT         1557 a         3275 c         3010 g         1579 t
ORIGIN      
        1 cgctcaacga cctggccaac gccttcgaca tcaccttccg cggcgactac ctctataaca
       61 ccgttgagaa cgagggcaac ttccgcaaca tcctgatcgg cgctgacggc tttggcgaca
      121 gcgcgctgac cgaggggcta gaccggctgg cactctacag cagccactcg ctgcaactgg
      181 ggccgtcggc ggcagcgctg ctatcggccg atgacaacac ctggtcgtcg gccaccgatc
      241 ggccgggcgg gctggtgctg gccgcgctca gcccggcggc gagcgatgcc gggcaactgc
      301 tggccatcgg cgacgttcac ttcctgaccg agccgtatag cactgtctat gacaacggtg
      361 cgttcgtcgc ccgcgtggcc gacttcctgg ccggcggcgg cagcacgggc acgctggccg
      421 gcttccccta tttcttccac tcgcctgttg acctggtcta tagtgacgac cccgacctgg
      481 ggccagacgc cttcgacgac atcatcgccc tgcaagccgc cttccgcggc gtggggctgt
      541 cgctggcgct ggccaacgag gccgccgccg accacgacgt gatctacgcc ggcctctata
      601 acaccgccga agacgtggcc gaactactcg atgccgccgg tgtctcgctg gccatccgcc
      661 cggccattgc ccgcccggca accacagcca ccactgaccc cgacgccacg ccgacgccat
      721 cccacggcac gcgcctgatc gagtcgccac tgggagacgt ggagatggcc gggacggcgc
      781 tgctgctgct ggctgacgac ggcacgcggc ggcaactggt cgttctggca gccagcaacg
      841 acggcctgga gaacgccctg acgcgactgc gcggcgcggc ctcggccgag ggcgtcaacc
      901 tgaccgactg tctcattcag gacaacctgg ccctgtgccc gaccggtgtg gccaacgagc
      961 cggtcgagta cgcgctggtg acgagcggcc cggccgacga gccgaccact gagtcggagc
     1021 agcccaacgg gagccaggca gacagccccg aaccgacgga caagggcgct atcgaactgg
     1081 gccagacgaa gacagccgag ttagccgccg gggaaatcca cgcctggaca ttcagagacg
     1141 gcccggcaac gatcgacatc actgtggacc ccgatgccga tatggacgcc gtgctggagc
     1201 tttatgcgcc cgacggcacg ctgatggcca atctcgattc cgcctttgcc ggcggcgtgg
     1261 aagagatgcg cgccgtggaa atacccgatg accgcccgta cttcatcatc gtccgcgact
     1321 tcttcaataa cggcggccac tatcagttga gcgtcgccgc cggcgagccg gcgcagccag
     1381 atcagccaga accgacgccg acacccgagc cgggaggcta ggagcggatg aattcaccgg
     1441 atatcctgtt gatcgtcctg gacacgcagc gagccgaccg gctgggctgc tatggccacg
     1501 accggcccat cacgcccaat ctcgatggct tcgccggcgg cggggcgttg ttcgagcggg
     1561 ccgtctcgcc ggcgcagtgg acgattccca gccacgcctc gctcttcacc ggtctatacc
     1621 ccacggctca cggcgtgacg cagtcgtcgc aaagcctcag cagcggccgg ccgcatctgg
     1681 cccaggtgct gcgccaactg ggctacgaga cggtcggctt ctgcaacaac ccgcttgtgg
     1741 gcgtactcaa caacggcttc aagcgcggct tcggcgcgtt ctataactat ggcggagcct
     1801 tccccagcct gccgcgccag tctaacccct ggccctggcc cctcaaccgc gtcgccgagg
     1861 gctacaccca gtttctgcgc cgcatctcct atcccatcca gaacttcttt ggccaatcgg
     1921 acctggcctt ccgcgtctcg ctccacgctt ttctgacgcc gctgtggtcg aaaatggcca
     1981 acttcaaggg gcagaacgag cgctcggtcg atgacgtcgt ccagtttctg gaggagcgcg
     2041 agacgaagcc ggccgaccgg ccgctgttcc tcttcctcaa tctgatggag acgcacctgc
     2101 ccttctggcc gccaggcgaa ttcatcgacc gcgtcgcccc ctatttccgc gaaagcaagg
     2161 cggcgcgtac tatcctgcgg acgtggaatc gcgaggccta ccgctgggcc gcgccactgg
     2221 ccgagccgct ggacgagctg gagtcacgtg tcctcaacga tctctacgat gccgaagtgg
     2281 cctaccaaga cgactacctg ggccgcctct tcgccgcgct ggcggcccgt ggacgcagcc
     2341 gcgacacgct gacgatcatc gttgccgacc acggcgacgg cctgggcgac cacggctact
     2401 ttggccacgc cttcgtcgcc tacgatgagc tggtgcacgt gcccctcatt gcccactggc
     2461 cggcgcggct ggccgcgggc gcgcgcgtgg ccacgccggt cagcacgcgg cgcgtcttcc
     2521 acacgctgat cgacgccgcc gcgccgacgc cggagacggc cgccgtcgtg gccgaggtgc
     2581 tggccgagga cgcgcccaac gtccgccgcc tgtcactgcg ccacacgctc aggggccgcg
     2641 acccggaggg agccaccgcc ttcagcgagg tctatccacc gctcaacttc gtccgcgcca
     2701 ttgagacgcg ccagccggag ttgctgacgc agttccgctg cctggccacg cggcgggccg
     2761 tcgtccgccc catgcgcgcg acctctggcg cggccgcgcc gctgaagctc atccacgtgg
     2821 acggcacagc cgacgcgctc tatgacctgg ccgccgaccc gcgcgaacag gacgacctcc
     2881 tgacgcagca gccggagatc gccgccgcgc ttgacctggc cctggagcag atggcccagc
     2941 gtatggagcg cgagcgcgag gcccagccgg ccggcgcggc ggtcgatctg gacagcgacg
     3001 agcttctccg gcagcggttg cgcgggctgg gctatctgga ataattcatt atccttctta
     3061 aacgggtgag acatgctaga cggtattcag attgacccgg ttgccttcac gattcccatc
     3121 ggcgacgggt ttcccattta ttggtatggc attcttgtca ccatcggtat cgccatcggc
     3181 gtgtggtggg gcgcgcgcga gatcgccagg cgcaaccaga acgtcgatga attcttcaat
     3241 ggtctgatcc tggtcatctt cagcggctac gccttcgccc gcctgaccta tgtcacgatg
     3301 gagtcgttgg ccgggcgcgg ggcgcagttc gagacgttct gggacctgat caacatccgc
     3361 tccggcggca tcaacatcct cggcgggttc atcggcgcgg ccgtggtggg cttcatctac
     3421 gtccgcgcgc gcggccttaa cttctggcaa tacgccgatg tggccggccc ggcggtgctc
     3481 atcgcccagg ccatcggccg ttggggcaac ttcatcaacc aggagttgta tggcccgccg
     3541 acgacgcggc cgtggggcat cctcatcgac gccgaccacc gcctgccgca atacgccgac
     3601 ctgacccagt tcccactgac tacccgcttc cacccgacgt tcctctatga gtctatctgg
     3661 ctgctgctgg gctttatcgt cctggtggcg ctcaacaacc gcaaccgcga caagtggcgg
     3721 gcaggcacgc ttttcggcct cttcctcatc tggtggggcg cagggcgggc cgtgctggag
     3781 ttcttccggc ccgaccaggt gactgtcggc gattcgccca tcacctactc tttcctcctg
     3841 gctctgggcc tggccatcgc cggcatctgg gtcgtcctgc ggcgcaacaa ccgccggccg
     3901 gaggtcttca cgcgccggcg gcgtcgccag ttgaaaccca agccacgccg cgaggcctag
     3961 cggcagccat ctgagcgcgc ccggcgcgca gcgaggcgga gatcatgcaa ttcgcaaacg
     4021 aatatggcga aacggcgctg accttcgacg atgtgttgct cgcgccgggc tattccgagg
     4081 tgttgccggc gcagacgcac acgcgcacgc gcctggtagg cgacctctac ctgagcatcc
     4141 ccattctctc ggcggcgatg gacaccgtca ccgaagcgcc aatggccatt gccctggcgc
     4201 ggctaggcgg gctgggcgtc atccaccgca acctgacgcc agaggagcag gctgaggaag
     4261 tcgataaggt caagcgctcc gagtcgggca tgatcgtcga tccgctgacg ctgcggccgg
     4321 acgatacgct ggccacggcc gaggcgctga tggcccgcta ccacatctcc ggcatcccca
     4381 tcaccgacgg cgacggccgc ctcgtcggca tcctgaccaa ccgcgacacg cgctttgtcc
     4441 agcccggcgc gcagcccatc gccgacttca tgacccgcga ggggctggtc accgcgccgg
     4501 tgggcaccag cctggaagag gccaaagcca tcctccaccg ccgccgcatc gagaagctgc
     4561 ccctggtgga cggcgacggc cgtctgaagg gtctcattac cgtcaaagat atcctcaaga
     4621 agatcgacta tcccgacagc gccgtggacg cgcgcgggca actgctctgc gccgcggcca
     4681 tcggcgtggg cgacaaggcg ctggcccggc tcgactgcct ggtcgaagcc ggtgtcgacg
     4741 ccgcggccat cgacacggcc cacggccaca cccgcctggt gctggagacc attcgcgccg
     4801 ctcgccgccg ccacccgcgc ctgcccatcc tggctggcaa cgtcatcgac ggggctggcg
     4861 cgcgggcgct gattgaggcc ggcgcggggg cgatcaaggt cggcatcggc gcggggtcga
     4921 tctgcaccac gcgcatcgtc gccggggcgg gcgtgcccca ggtctcggcc atcgccgccg
     4981 tcgccgccgt ggcccgcgcg ctggacacgc cggtcatcgc tgacggcggc atcaaataca
     5041 gcggcgacat cgtcaaggcc ctggctgccg gggccgacgc ggtcatgctc ggctcgctgc
     5101 tggccggtct ggaggaaagc cccggcgaga tcatcctcta cgagggccgc cgctacaagg
     5161 tctaccgcgg catgggcagc atgggcgcga tgcagggctt cggcgcggat cgctatggca
     5221 gtggcgtggc cgcggccgat gggctgcaca cgcccgctgg tggccaggtg cgcgacaagc
     5281 tcgtacccga aggcattgaa ggacaagtgc cctataaagg ccgcctgggc gacgtcgtct
     5341 accagatgat gggtggcgtg cgctccggca tgggttacgt cggcgcggcc gacctggccg
     5401 agttgcgcgc caaagcccgc ttcgtgcgca tcaccaacgc cggcctgctg gaaagccacc
     5461 cgcatggcgt cctcatcacc aaagaagcgc ctaattacca ggcgcgccgt tagcgtcctg
     5521 ccgggaggct gccatcatga ataaaggcgc tgtctacgcg gccggagcct atatcctctg
     5581 gggcctgctg cccatctact ggaagacgct ccacagcgtg ccgtccacgc agatcgtcgc
     5641 ccatcgcctg gtctggtcgc tgggcttcgt cgctctggtg ctgacggcgc gccacaattg
     5701 gggctggctg ggtggcgtgc ggcgccggcc acgtgtctta ctgaccttcg ccctgtctgg
     5761 cgcgctgctg acggccaact ggatggtcta catctggggc gttaacgccg gtttcatcgt
     5821 cgagacgagc ctgggctact tcatcaatcc gctggtcaac gtcatgctcg gctacgtcat
     5881 cctcaaggag cggctgcgcc cgccgcaatg ggcggcgctg gccatcgccc tgggcggcgt
     5941 gctctacctg acgttcagct acggcgcgtt cccgtggatc gccctgacgc tggccttcag
     6001 cttcggcttc tatgggctca ttcgcaagac ggcggcgctg aattcggccg aaggactgtt
     6061 cctggagacg gccctcctct tcctgccggc gctgggcttc ctggtgctgc aagaagtgcg
     6121 cggcgttggc gcgctggccc acaccgacgc acgaacgacc atcctgctca tcggcgcggg
     6181 cgcggtgacg agcatcccgc tgatcctctt tgccgccggc gcgcgacgca tcaccctgac
     6241 cacgttgggg ctgctgcaat acatcgcccc aacgctgcaa ttcctcatcg gcgtgctcat
     6301 ttaccacgag ccgttcggcg cgggccgggt cgtgggcttt gggctgatct ggctggcgct
     6361 gatcctctat acgaccgaga gcctggccca ttaccggcga attgccgcac cagtgcagac
     6421 gtaaccccga caggtatcgc ccttgtcaag gtaggctata attagggtat ggtcactatc
     6481 gtatccatcg atctcatttc gacagaccag accgtgcgcg gcgggaaagc ctgcatcgcc
     6541 ggtacgaccc tacgcgtgat cgatgtagtc atggcatcta tcttccatgg gcgcaccccc
     6601 agtgccattg cgtccgactt cgacgtgtcg ctggcccaag tccacgccgc cctagcctac
     6661 tattaccagc ataagaccca actggacgaa gatatacgcg cccagctaga ccgcgcgcaa
     6721 gcgttcaaac aagagagact tggcagccaa cacgcttcgt tattatctgg atgagaacct
     6781 gccggttgag attgcccgtc aacttcggct acgtggcata gatgctgtca ctgtgcgaga
     6841 cctgggacgc ctgggggagc cggacagcaa tcacctccat cgtgccaccg atatgggcat
     6901 ggtcttatgc acgtttgaca cggacttcat tgaactggta gcggccggac agcagcatgc
     6961 cggcattgtt ctgggccaac aggatgtaca tgagattggc gcatgggttc gctatctgga
     7021 actgatgcat acggtttact ctgccgaaga catgcaagac catattgagt atctctaggc
     7081 cgcgctccaa tcgatgccca cgacggatca ccaaacctac gcctcgccgt ttacctggcg
     7141 ctatggcagc gagccgatgc gctatctctg gtcagagacg cacaagcggc ggctgatgcg
     7201 gcaggtatgg gtggccctgg ccgcggcgca gcacgtcgcc gggctggtga cggccgagca
     7261 gctggccgac ctggaggccc acgtcgatag cgtcgatatc ccgcgggctt tgcagatcga
     7321 acgggagacg cgccacgacg tggtggccga aattcgcgcc ttcgccgagc agtgccgcgt
     7381 cggcggcggc atcatccatt ggggagccac cagcgccgac attaccgaca acgtcgatgt
     7441 gctgcgccag cgcgaggccg cccgcctgct ggccgagcaa ctccgccggc tgctgctggc
     7501 cttcgcccgg cgcattgagg agacggccga cgtcgtcatc atggcccaga cccacatgca
     7561 gccggccgag ccgacaacgt tgggctatcg cctggccatg tacgcccagg acctggccga
     7621 ggacgagagc cgcctaaacg gcctcatcag tgacctgcgc ggcaaggggc taaagggggc
     7681 cgtgggcacg caggcaggct tcatcgaggt gctcgatgtc tgcgacatga ccgtcgtcca
     7741 actggaggaa ctggccatgg cccggctcga cctgccctat tttcccgtgg ccgggcagac
     7801 ctacacgcgg cagcaagacc tgcgcgtgca gagcgttctg gccggcatcg ccgccgcgct
     7861 gcacaagttc gccctcgact gccgcattct ccaatcgcca cccttcggtg aatggtcgga
     7921 gccgttcggc cagcgtcagg tcggctcgtc ggccatgccc ttcaagcgca accccatcaa
     7981 cgccgaaaac atctgctcgc tggcccgctt tgtggccgcg cagacagccg tggcctggga
     8041 caacgccgcc caggctgtgc tggagcgcag cctcgacgat agcgccaacc gccgcctgtt
     8101 cctgccggag agctttctgg ctatggacga gatgctgcgg cgggcaacgg cgctggtgga
     8161 gggtctgatc tttgaccatg aacagatggc gcgcaacgtg acccgctatg gtcctttcgc
     8221 ggccacggag cgggtgctga tggccgccgt gcgcgccggg gccgaccggc aggcagcgca
     8281 cgaatggcta cgcgaagcca gcctgcgtgc ctgggaggcc attcgccacg accaggccaa
     8341 ccccctgacg gagctactgt cggccgacgc tcgcctgacg gcctacctgc cggccgagac
     8401 cgtcagcgcc ctcatggacg cgggcggcta cgtgggcacg gctgccgagc gagcgcgggc
     8461 gctggccgat acgctcaaga ccccgctccc taacccccgc cgcctgtagt ttgtctggca
     8521 acttctcgcg cccctcggcc gacagggcta cccgccttca atccggtcac cgcgctgagc
     8581 atctcgccgg cgttgattgc ggccaggtcg ccgtggaact gcttgtcgag gctgaaccgg
     8641 cccaggcctc aggccccggt cgccggctgg gggttacgct tgacttcaaa ttgacttgag
     8701 ccctgaccgg ccgcatgctg cgcaaagtag cacagcctgt taggctgtcg tcgccggccg
     8761 cagccacagc ctgacaggct gtgctacaga cagcctgacc ggccgcatgc tgcgcaaagt
     8821 agcacagcct gttaggctgt cgtcgccagc cgcagccaca gcctgacagg ctgtgctaca
     8881 gacagcctga caggctgtgc tacaggctgt ggacggcatc aaacaccgcc ggtgctgctc
     8941 ccaggatggc caggtcgata tcacccagca acgctccgtc gccggtggct gcgcgtgggc
     9001 gtggctcgtc agccgaatga gacggccaac cgccgctggc agaccgctgc cggtgggagc
     9061 ctgggtggct gccgccaggc ggtcgccggc cagtgccctg agcgtgacct ccgccagttc
     9121 ggccgaccgc gtctcgttgt cgccgcgttg cgggtcgtag atggcgtcat ggaaccagat
     9181 ggccagctcg acggcggccg gccaggccgc cagatggcgc acagcgtcga agtggcgcag
     9241 gcaatcggtg atgtggccga gcgtgtggta ggcgcgctgc gggtcggcgt agcgggagac
     9301 gaggtcggtg tagacgcggg cgaccgtggc ctcgtccgcg cccggccaga gcgtccgcca
     9361 ctgcgtggcc aatggttcag ccgccactac tggcaaaacg cccgcgccac ggccagatag
     9421 a
//
LOCUS       DAOSMQ010000062         9377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7290_length_9377_cov_3.944003, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000062 DAOSMQ010000000
VERSION     DAOSMQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7290_length_9377_cov_3.944003"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PK829_03390"
     CDS             <1..915
                     /locus_tag="PK829_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40292.1"
                     /translation="MIHLIFNFPGRTVYTHAMPGQPWSTPVALSENYAAMPLLVADTD
                     DSLFGIVPEIVDYHFVLKQLASAPAGQAGTATLSQQLTIPATMSHPTLSFMKRVLGNN
                     GGGATVVARIQEGGNPPQSFPITGDARWSLGWLDVSAWAGKTVVVTFAVEQPADAPPA
                     QLYLDAITLGSASPQLWAQLRSPGQAQPGATVAGMLAAGNRGAADADAAVLTLTLPDG
                     LSLAAATPPAEVTGNTARWSLGALAAGEERSIALTLAVAPGAPLGQRRTLRAELTAAT
                     PEVELADNRSEQAVFFGWQMLMPALFKN"
     gene            1088..4081
                     /locus_tag="PK829_03395"
     CDS             1088..4081
                     /locus_tag="PK829_03395"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOU40293.1"
                     /translation="MPRSIPSSRHLLALFTLLALLVAALQLAPRAWAADFSAANETEL
                     AAAITAANAAGAGTHTIALTADITLTAPLPALNNTAGTIALDGGGHTLNGGGAGRVLL
                     VARDAAATATDLTITGGNTAGDGGGILVRGRLSLTDVTISGNAANRGGGIFVDGAPGS
                     TVELSLLRVEISSNAANLDGGGIAATGDEGQVTITAADSRLDDNSAAGLGGGILASGF
                     SGAVDVALARTTLAGNRALHGAGVFLNGNGGQVELVAAASTLSGNRANGVGGGLYLNG
                     NDGLAAADVLNSTFSGNEAANGGGIAASDNTGTAELTLRYTTVAANTANTGSALRLVS
                     GAAANVSASILSSGGGAGKACAILSGAALTSGGYNLDNDDSCGLATAGDVANGAPDLA
                     ALALNAPGTTATHALGAASDALDAIPAGAAGCGAAVAVDQRGAARPAPATACDMGAYE
                     SDAGEPPTATATTTATATATPTATATVSATPTATVSPTPGPAPTPTASATPPSGCVPP
                     YNPATEAALNAAIACVNAAGTGTHTITLAADLALTGATTPLSNPAAMELQVVGAGHAI
                     NGNGHGPILAVAAGTTARLSGLTLRSGAAADGAAVNNAGILTIENSQLRDNTATGRGG
                     AVFNRGRLTLKGVTLTGNSAPTGGGVASAAESADATVIVLDSNLTANTAGNGGGLWAR
                     GTGHTAAVTLRNTTFADNTGTAGAGGAALLAGPDGRLTATIGATRFTGNSGGGLSALA
                     LGGDAAVNVDGTTFAGNTATDGAGVRLEVQDKGTAELLLSKTTLSGNAATGAGGGVHA
                     TASGGGTAKLTLFNATLSGNSAGGAGGGLSLATNGGATGANVVYTTLAGNTANGGGGG
                     IHTAGSASATLTATIITNGAGAGPDCVRAGGSIISTGYTLDGDNSCYLAPDGNDLPGM
                     AAAGLLPLAVYAPGGTATHALSATNPAADRIPVGAAGCGTAVTTDQRGAPRPGAAGGR
                     CDLGAYERGAGDGAARRVFAPVVLR"
     gene            complement(4185..5519)
                     /locus_tag="PK829_03400"
     CDS             complement(4185..5519)
                     /locus_tag="PK829_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU40294.1"
                     /translation="MTQPPIPGFRDVPRTGVIYVMHRAAELGFSYDAPDWANLGQGAP
                     ETGPLPGAPPRIDTVTMNPRQHEYGPITGQTALRRKVADLYNTLYRQGKRSQYTWENV
                     SISGGGRVALARIAAALGNINMGHFLPDYTAYEELLSIFKAFIPIPILLDPDLGYQAP
                     LEMVKREIQGRGLKAMLLSNPANPTGQVIDGDRLAQWVQLARNYRCALILDEFYSHYI
                     YNGKQGGGGAGELGSISEMKNGSPLHPGSPAPLPKMVSAAAQVEDVDRDPVILVDGLT
                     KNWRYPGWRISWTLGPKEVIDAIASAGSFLDGGANNPFQAEVLGLLEPECVIQETRAI
                     QTAFRAKRDYTLDRLYKMGVLVEVEPAGTFYVWANLSQLPPPLNDGMQFFEAGLEERV
                     ITVPGVFFDVNPEKRRTYARYRNYCRISFGPEMEVVARGLDALERVVGRHAS"
     gene            5686..6693
                     /locus_tag="PK829_03405"
     CDS             5686..6693
                     /locus_tag="PK829_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU40295.1"
                     /translation="MSKSNPTSGQRPSRSELRASLIWPVVISIAIWVAGLLIYIFAPG
                     RFDALVSGLIAVGLVIYLLYYQRSQHLSPGERLGALLLATPAVAGIAYGLVRGDALFA
                     ITGVSASLLLLSAQRVLTTPISYRMARRAFMRGRLETALDLVDKAIGARPHFWESYQL
                     RSLIYLSALQFVAAERDARKALELRPDAHPVYNTLGQIYLAESRYDAARDAYSRAIDL
                     SPKHALYHYHHGLALYRLGQPRAAAEALAAATRLSLPHVTYELQAYYYLGRALEGNGE
                     IEKAEEVFADMVKFKEGLETLKGELKGQPDFPELPALRADLRDLEKRLAAAEREAVKR
                     D"
     gene            7046..>9377
                     /locus_tag="PK829_03410"
     CDS             7046..>9377
                     /locus_tag="PK829_03410"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOU40296.1"
                     /translation="MPPWLASLILKRGGGALARFAVHYQQLASMPRAWRQRLRRKLAV
                     SLSSAALIMALAGPVALPMSAAPAAPDATIVVVNGEVADIQNNKCSLIEAIINARTDK
                     AGQRRADCAAGNLNGPDTISLPANGNFVLTAVHNEQFGSTGLPVITSAVTIDGHGATI
                     RRSSVSGTPNFRILAVDANGALTLNDTTISNGQAIYYAAKGGGILSYGDLTLSGSTIS
                     DNAVNGEGGGSGGGLYIAANSQATISDSQIINNSAASAYGTFGGGIAVYGQATILNTT
                     IAGNVNAGSEGGGEGGGIAVMGQATISGSTISGNSAQAYYEGYSYDWHPGVGGGLSNS
                     GTTTIVNSTISANTASNGGGLANVFDGVLTVTQSTVTGNDAHETVGEYDTAGGSGGGL
                     FAGPDYGIDPSGTTTLKGVILSGNTADIAAPQALFENSGGTAHMIVNAFNVFGQNGNA
                     GLAGFSKGATDVVPSVGLAAVLSPLANNGGPTQTHALPAGSPALDLAPNASCTAAPVN
                     GVDQRGEPRNQNGKDGATNNECDAGAYERGGTVAAMGFYLSASGSGAVGGVAFAPGDI
                     IKFTPPSTWAMHFDASDVGITKNVTAVAIEANGDILLSLAAGQNVPGVGAVQPQDVLR
                     FTPTSTGNNTAGSFAMALDGSANLLTTSGEKIDALARTDGRLAISTVGAAAVKRADNS
                     VLKAQDEDALGFNLNSGQWSEFFNGTAIPGLAAEDVNGLWIDPATGDLYISIVGAFNL
                     GGVKGDGKDIVKLSPSGAPGGYTPSLFWDGSANGFPT"
BASE COUNT         1516 a         3431 c         3106 g         1324 t
ORIGIN      
        1 atgattcacc tcatcttcaa ttttccaggc cggaccgtct acacccacgc gatgcccggc
       61 cagccctggt cgacgccggt cgcgctgagt gaaaactacg cggcaatgcc tctgctggtg
      121 gccgatacgg acgattcgct gtttggcatc gttcctgaaa tcgtcgatta ccacttcgtg
      181 ttgaaacagt tggcgagcgc gcccgccggc caggccggca cggccaccct ctcgcagcag
      241 ctcaccatcc ccgccaccat gagccacccg acgctctcct tcatgaagcg cgtcctgggc
      301 aacaacggcg gcggcgcgac ggtggtcgcg cgcatccagg agggcggcaa cccgccacag
      361 agcttcccca tcaccggcga cgcccgctgg tcgctgggct ggctcgacgt gtcagcgtgg
      421 gcgggcaaga cggtggtagt caccttcgcc gtcgagcagc cggccgacgc cccgccggcg
      481 cagctctacc tcgacgccat caccctcggc tcggccagcc cgcaactctg ggcgcaactg
      541 cgctcgccgg ggcaggcgca gccgggagcg acggtcgcgg gcatgctggc cgccggcaac
      601 cgcggcgcgg ccgacgccga cgccgccgtg ctgacgctga ccctgcccga cggcctgtcg
      661 ctggcagcgg ccacgccgcc ggcggaggtc acgggtaaca ctgcgcgctg gtcgctgggg
      721 gcgctggccg ccggcgagga gcggtcgatt gccctgacgc tggccgtcgc cccgggcgcg
      781 ccactgggcc agcggcgcac gctacgcgcc gagttgaccg cggccacgcc ggaagtagag
      841 ctggccgaca accgcagcga acaggccgtc ttcttcggct ggcagatgct tatgcccgct
      901 ttgttcaaaa actagggtcg cgagggaaag aggggtgggc agtggccagt tctacggcct
      961 actgcccact gcccactgcc cactgcccac tgacaatgag ttttcacgtc gtcttcgctc
     1021 gcgagagcaa caatgggccg tatactgttg gcgcattgtg caacaaacca tcatcaggaa
     1081 gacgactatg ccgcgctcga ttccttccag ccgacacctc cttgccctgt ttacgctact
     1141 ggccctgctc gtcgccgcgc tgcaactggc cccgcgggcc tgggcggccg atttctccgc
     1201 cgccaacgaa accgaactgg ccgcggccat caccgccgcc aacgccgccg gcgcgggcac
     1261 gcacaccatc gctctgacgg ccgacatcac cctgaccgcg cccctgccgg cgctgaacaa
     1321 caccgccggc acgatcgccc tcgacggcgg cggccacacc ctgaatggcg gcggcgcggg
     1381 ccgcgtcctg ctggttgccc gcgacgccgc ggccacggcc actgacctga ccatcaccgg
     1441 cggcaacacc gccggcgacg gcggcggcat ccttgtgcgc ggccggctca gtctgaccga
     1501 cgtgaccatc agcggcaacg cggccaatcg cggcggcggc atcttcgtcg atggcgcgcc
     1561 gggcagcaca gtcgaactgt ccttactgcg cgttgagatc agcagcaacg cggccaacct
     1621 cgacggcggc ggcatcgctg ccaccggcga cgagggacag gtgacgatca ccgccgccga
     1681 ctcgcgcctg gacgacaaca gcgcggccgg gctgggtggc ggcattctgg ccagcggctt
     1741 ctccggcgcg gtcgacgtgg ccctggcgcg cacgaccctg gccggcaatc gagcgctcca
     1801 cggcgcgggc gtcttcctga acggcaacgg cgggcaggtg gaactggtcg ccgcggccag
     1861 cacactgtct ggcaaccgcg ccaacggcgt cggcggcggc ctatacctga acggcaacga
     1921 cggcctggcc gcggccgacg tgctcaacag caccttctct ggcaatgaag cggccaacgg
     1981 tggcggcatc gccgccagcg acaacaccgg cacggccgaa ctgaccctgc ggtacaccac
     2041 cgtggccgcc aacacggcca acaccggcag cgccctgcgg ctggtctccg gcgcggcggc
     2101 caacgtgagc gcgtccatcc tcagcagcgg cggcggcgcg ggcaaggcct gcgccatcct
     2161 cagcggcgcg gcgctgacct ccggcggcta caacctggac aacgacgact cctgtggcct
     2221 ggccacggcc ggcgacgtgg ccaacggcgc gcccgacctg gcggcgctgg ccctcaacgc
     2281 cccgggcacg accgccaccc acgccctggg ggccgccagc gacgccctcg acgccattcc
     2341 cgccggcgcg gccggctgcg gcgcggccgt ggccgtcgac cagcgcggcg cagcccgccc
     2401 cgccccggcg actgcctgcg acatgggcgc gtatgagagc gacgcgggcg agccgccaac
     2461 ggccacggcc acgaccaccg ccacagctac agccacgcct accgccacgg ccactgtgtc
     2521 cgccacgccg acggcgactg tctcgcccac gccggggccg gcgcccacgc cgaccgcctc
     2581 ggccacgccg ccaagcggtt gcgtgccgcc ctacaatccg gcgacggagg cggcgctcaa
     2641 cgcggccatc gcctgcgtca acgccgccgg cacgggcacg cacaccatca ccctggccgc
     2701 cgatctggcc ctgaccgggg cgacaacgcc gctgagcaat ccggcggcga tggaactgca
     2761 agttgtcggc gcgggccacg ccatcaacgg caacggccac ggccccatcc tggccgtggc
     2821 cgcgggcacg acggcgcggc tgagcggcct gacgctgcgg agcggcgcgg cggccgacgg
     2881 cgcggcggtc aacaacgccg gcatcctgac tatcgagaac agccagttgc gcgacaacac
     2941 cgccaccggg cgcggcgggg cagtcttcaa ccgcggtcgc ctgacgctga aaggcgtcac
     3001 cctgaccggc aacagtgccc caaccggcgg cggcgtggcc agcgcggccg aatcagccga
     3061 cgcaacggtt atcgtgctcg acagtaacct gaccgccaac acagccggca acggcggcgg
     3121 cctgtgggcg cggggcaccg gtcacacggc cgcggtgacg ctgcgcaaca cgaccttcgc
     3181 cgacaatacg ggcacggccg gcgcgggcgg cgcggcgctg ctggccgggc cagacggccg
     3241 gctgacggcc accatcggcg cgacacgttt caccggcaac agcggcggcg gcctgtcggc
     3301 tctggccctg ggcggcgacg cggccgtcaa cgtcgatggc acgaccttcg ccggcaacac
     3361 ggccacggac ggggccggcg tgcggctgga ggtgcaagat aaaggcacgg ccgagctgct
     3421 gctgagcaag acaaccctat cgggcaacgc ggccacgggc gcgggcggcg gcgtccatgc
     3481 cacggcctct ggcggcggca cggccaagct gaccctcttc aacgccaccc tttcgggcaa
     3541 cagcgccggc ggcgcgggcg gcggcctgtc gctggcgacc aacggcggcg cgaccggggc
     3601 caacgtggtc tacacgacgc tggccggcaa caccgccaac ggcggcggcg gcggcatcca
     3661 caccgccggc tcggcctcag cgacgctgac ggcgacgatc atcaccaacg gcgcgggcgc
     3721 cggccccgat tgcgttcgcg ccggcggcag catcatctcc accggctata ccctcgacgg
     3781 cgacaacagc tgctacctgg cgccggatgg caacgacctg ccgggcatgg ccgccgcggg
     3841 gctgctgccg ctggccgtct acgcccccgg cggcacggcc acccacgccc tcagcgccac
     3901 caacccggcg gccgaccgca tccccgtcgg cgcggccggc tgcggcacgg ccgtaacgac
     3961 cgaccagcgc ggcgcgcccc gaccaggggc ggccggcggc cgctgcgacc tgggcgcgta
     4021 tgagcgcggc gcgggcgacg gcgcggcgcg gcgggtgttt gcgccggtgg tgctgcgcta
     4081 gggggtcgag cggggcgcga atgacgcgaa tgggatgcgg attagcgcgg agcaggccgc
     4141 agaggtctca gagagcctct gcgggccggg ctggggccac tgtcctagct cgcgtgccgc
     4201 ccgacaaccc gttccagcgc atccaacccc cgcgccacca cctccatctc cgggccgaag
     4261 ctgatccggc agtagttgcg gtagcgggcg taggtgcggc gcttctccgg gttgacgtcg
     4321 aagaagacgc ccggcacggt gatgaccctc tcttccagcc cggcctcgaa gaactgcatg
     4381 ccgtcgttga gcggcggcgg cagttgcgac aggttggccc agacgtagaa cgtgcccgcc
     4441 ggctccacct ccaccagcac gcccatcttg tagagccggt cgagggtgta gtcgcgcttg
     4501 gcgcggaagg cggtctggat ggcccgcgtc tcctggatga cgcactccgg ctccagcagc
     4561 cccagcacct cggcctggaa ggggttgttg gccccgccgt cgaggaagga gccggcgctg
     4621 gcgatggcgt caatgacctc cttggggccg agcgtccagc tgatgcgcca gcccgggtag
     4681 cgccagttct tcgtcagccc gtcaacgagg atgaccgggt cgcggtcgac gtcttccacc
     4741 tgcgccgcgg ccgagaccat cttcggcagg ggggctgggg agccggggtg caggggggag
     4801 ccattcttca tttcgctgat actccccagc tcccctgctc ccccgccccc ctgcttccca
     4861 ttatagatat agtgcgaata gaactcatcc aggatgagcg cgcagcggta attgcgggcc
     4921 agttgcaccc actgggccag ccggtcgccg tcgatgacct ggccggtggg gttggccggg
     4981 ttgctcagca gcatcgcctt cagcccacgc ccctgaatct cgcgcttgac catctccagc
     5041 ggggcctggt agcccaggtc ggggtcgagc aggatgggga tggggatgaa cgccttgaag
     5101 atgctcagca gctcctcgta ggcggtgtag tcgggcagga agtggcccat gttgatgttg
     5161 cccagcgccg cggcgatgcg cgccagggcg acgcggccgc cgccggagat gctgacgttc
     5221 tcccacgtgt actggctgcg cttgccctgg cggtagagcg tgttgtagag gtcggccacc
     5281 ttgcggcgca gggcggtctg gccggtgatg gggccatact cgtgctggcg cgggttcatc
     5341 gtcaccgtgt cgatgcgcgg cggcgcgccc ggcagcggcc cggtctccgg cgcgccctgg
     5401 cccaggttgg cccagtcggg cgcgtcgtag ctaaaaccaa gttcggccgc gcggtgcatg
     5461 acatagatca cgcccgtgcg gggaacgtcg cggaaaccgg gaataggggg ttgcgtcatg
     5521 ggtatgattg ccttatgatt taattcgccg ccgaggcagc ggcctatttt ggagcgaagc
     5581 ggcgggaaag tcaaccaggg ccgtggcgcg attctccgaa tcgcgtcttt gccgccagta
     5641 gagttagcgc gattcgggag aatcgcgcca cacgtagagg cagacgtgag caaatccaat
     5701 cccacctccg ggcagcgtcc ttcgcgcagc gaactgcgcg ccagcctcat ctggccggtc
     5761 gtcatctcta tcgccatctg ggtcgccggc ctgctgatct atatcttcgc ccccggccgc
     5821 ttcgacgccc tcgtctccgg cctcatcgct gtgggcctgg tcatctacct gctctattac
     5881 cagcgcagcc agcacctttc ccctggcgaa cggctggggg cgctgctact ggccaccccg
     5941 gccgtggccg gcatcgccta tggcctggtg cgcggcgacg cgctctttgc catcaccggc
     6001 gtcagcgcca gcctgctgct gctcagcgcc cagcgcgttc tgaccacgcc catctcttac
     6061 cgcatggccc gccgggcgtt catgcgcggc cggctggaga cggctctcga cctggtggac
     6121 aaggccatcg gcgcgcggcc ccacttctgg gagagctacc agttgcggtc gctcatctac
     6181 ctgtcggccc tgcaattcgt cgccgccgag cgcgacgccc gcaaggcgct ggaactgcgc
     6241 cccgacgccc acccggtcta caacaccctc ggccagatct acctggccga gagccgctac
     6301 gacgccgccc gcgacgccta cagccgggcc atcgacctgt cgcccaagca cgcgctctac
     6361 cactaccacc acggcctggc cctctaccgg ctgggccagc cccgcgccgc ggccgaggcg
     6421 ctggccgcgg ccacccgcct gagcctgccc cacgtcacct atgagctgca agcctattac
     6481 tacctcggcc gcgccctgga gggcaacggc gagatcgaaa aggctgaaga ggtcttcgcc
     6541 gacatggtca agttcaagga ggggctggag acgttgaagg gcgagttgaa gggccagccc
     6601 gacttccccg aactgccggc cctgcgcgcc gacctgcgcg acctggagaa gcgcctggcc
     6661 gcggccgagc gcgaggcggt gaagagggac tagggattag gggataggga atagggaaga
     6721 gcgctcttcc ctattcccta tcccctagcc cctatcccct agcccctagc cttaggaatg
     6781 tcgccggctt tacacctgcc tgcgctactc tgtgctatac tcctatcagg gagtactgtt
     6841 agccctcccc tgtaaccaaa tcagtgccgc cgaaccgggc gccctccact gtggcgcagg
     6901 agccattttc cccaccacaa tgcaccgcct accggctggc tatcgtgcgg cgacgggctg
     6961 ctggttgcgg ccggtggtca cgcacgggcg aaaaggagcc atagccgtgt attcgtatcg
     7021 ttcgccttcg caaccgcgtg aagagatgcc cccctggctg gcaagtctca tcctcaagcg
     7081 gggcggcggc gcgctggcgc gcttcgccgt ccactatcag cagctggcca gtatgccccg
     7141 cgcctggcgg caacgcctgc gccgcaagct ggccgtcagc ctcagcagcg ccgcgctgat
     7201 catggccctg gccgggccgg tcgccctgcc catgtcggcc gcgccggccg cgcccgacgc
     7261 caccatcgtc gtcgtcaacg gcgaggtggc cgatatccag aacaacaagt gctcgctcat
     7321 cgaggcgatc atcaacgcgc gcacggacaa agccggccag cggcgcgccg actgcgccgc
     7381 tggcaacctg aacggtcccg acaccatctc gttgccggcc aacggcaact tcgtgctgac
     7441 cgccgtccac aacgagcagt tcggctcgac cggcctgccg gtcatcacca gcgctgtaac
     7501 gatcgacggg cacggcgcga ccatccgccg cagcagcgtc agtggcacgc ccaacttccg
     7561 catcctggcc gtcgatgcca acggcgcgtt gacgttgaac gatacgacaa tttccaacgg
     7621 tcaggcaatt tactatgctg ccaagggcgg cggcatcttg agctacggcg acctgaccct
     7681 gtcgggtagc accatcagcg acaacgcggt caatggtgaa ggcgggggca gcggcggcgg
     7741 tctctacatc gccgccaaca gccaggcgac catcagcgat agccagatta tcaacaactc
     7801 ggccgcatcg gcttacggca ccttcggcgg cggtatcgcc gtctatggcc aggcaacgat
     7861 cctcaacacc actatcgccg gcaatgtcaa tgcaggcagc gagggcggcg gcgaaggtgg
     7921 cggcattgct gtcatgggcc aggcaaccat tagcggtagc acgatcagcg gcaacagcgc
     7981 ccaggcctac tacgagggct actcctacga ctggcatccg ggtgttggcg gcggtctcag
     8041 caacagtggc acgaccacca tcgtcaacag caccatcagc gccaacaccg ccagcaatgg
     8101 cggcggcctg gccaatgtct ttgacggtgt cctgaccgtc acccagagta cggtgacggg
     8161 caacgatgcc cacgagacag tcggcgagta tgacacggcc gggggcagcg gcggtggcct
     8221 gttcgccggg ccggactatg ggatcgatcc ctccgggacg accacgctga agggcgtcat
     8281 cctctccggc aacacggccg acatcgccgc cccgcaagcg ctgttcgaga attcgggcgg
     8341 cacagctcat atgatcgtca acgccttcaa cgtcttcggc cagaacggca acgccggcct
     8401 ggccggcttc agcaagggcg cgaccgacgt cgtgccgtcc gttggtctgg cggctgtcct
     8461 gtcgcctttg gccaacaacg gcggcccgac gcagacccat gccctgccgg cgggcagtcc
     8521 cgccctcgac ctggccccca atgccagttg cacggcggcg cccgtcaatg gcgtcgacca
     8581 gcgcggcgaa ccgcgcaacc agaacggcaa ggacggcgcg accaacaacg agtgcgacgc
     8641 cggggcctat gagcgcggcg gaacggtcgc ggcgatgggc ttctacctgt cggccagcgg
     8701 cagcggcgcg gtcggcggcg tggcctttgc ccccggcgac atcatcaagt tcacaccgcc
     8761 gagcacctgg gcgatgcact tcgacgcctc cgacgtaggc atcaccaaga acgtcaccgc
     8821 cgtcgccatt gaggccaacg gcgacatctt gctgtcgctg gccgcggggc agaacgtgcc
     8881 cggcgttggc gcggtgcagc cgcaggacgt gctgcgcttc acgcccacca gcaccggcaa
     8941 caacaccgcc ggctcgttcg ccatggccct cgacggctcg gccaacctgc tgaccaccag
     9001 cggcgagaag atcgacgccc tggcccgcac cgacggccgc ctggccattt cgaccgtggg
     9061 cgcggcggcg gtgaagcggg ccgacaacag cgtcctgaag gctcaggacg aggacgcgct
     9121 gggcttcaac ctgaacagcg gccagtggtc ggagttcttc aacggcacgg ccatccccgg
     9181 cctggccgcc gaggatgtca acggcctgtg gatcgacccg gccacgggcg acctctacat
     9241 cagcatcgtc ggcgcgttca atctcggcgg cgtcaagggc gacggcaagg acatcgtcaa
     9301 gctctcgccc agcggcgcgc ccggcggcta cacgccgtcg ctcttctggg acggctcggc
     9361 caacggcttc ccgacga
//
LOCUS       DAOSMQ010000063         9121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7564_length_9121_cov_4.459298, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000063 DAOSMQ010000000
VERSION     DAOSMQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7564_length_9121_cov_4.459298"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PK829_03415"
     CDS             complement(<1..146)
                     /locus_tag="PK829_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOU40297.1"
                     /translation="MIAPTSFFSDYGGHIRILEETRALQARGVEVAIVTYYKGNDVPG
                     IDIRR"
     gene            complement(184..1179)
                     /locus_tag="PK829_03420"
     CDS             complement(184..1179)
                     /locus_tag="PK829_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOU40298.1"
                     /translation="MKAQTVTIVGMGRTGVSIGQALRAADAGLTIVGHDRSRELAEDA
                     KDKWAAVDRVEWNLVAAAMAADILVLAVPLAELENTLMVISEDVQPHALVLDVAPLKT
                     QSLRWADKHLRSGHYIGVVPVLSAAYLNDGRDEPAAATPDLFRNSIFCLLPSAKAAPG
                     AVDTAVNFGRLLGALPYFVDPLEYDSLAQAVETLPGLVAAALFGAVQKSAAWRDILRF
                     ANPSFAVATQPLLHGADITAMALNDRAAILRWLDAMLNELTLIRRLIQEGDRDAIDLT
                     LAGLLVQRERWMREREANEWVEVADSPVERPSLGEQVLGGWLSNKLKPSQRDERR"
     gene            complement(1192..1698)
                     /locus_tag="PK829_03425"
     CDS             complement(1192..1698)
                     /locus_tag="PK829_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU40299.1"
                     /translation="MGLRYRLRQLKNNLTAGPLSEVARQEVAVLLSPAERALFTRLSL
                     ADQWHSVRVLGDLRAAGYNDPDLLAAALLHDVGKTRYPLTAWDRTVIVVGVALLPRRA
                     ARWGQGPPDGWRRPFVIRARHPEWGAAMAAAAGTRAAVVDLIRRHADRPGEGDDVLRA
                     LQWADDQN"
     gene            complement(1700..2338)
                     /locus_tag="PK829_03430"
     CDS             complement(1700..2338)
                     /locus_tag="PK829_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOU40300.1"
                     /translation="MLNLPDVSTIIARIVVLIIAFTIHELSHAVTADYLGDPTPRRMG
                     RITLNPLKHLDPIGTLLLIVAGFGWAKPVMVNPMNMRGNPRTSMAIVAAAGPVSNLLM
                     AAIAAVFFRLGVVSFTQTSSALSPSLLLSEFIWINLILAFFNLIPIPPLDGSKILYAL
                     LPSELVYRLRPLEQFGFILLMAVVFFVPAVLNLFVGLPAAALLQLLIGGGSL"
     gene            complement(2540..3595)
                     /locus_tag="PK829_03435"
     CDS             complement(2540..3595)
                     /locus_tag="PK829_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40301.1"
                     /translation="MALLEVRDVAKSFAPGTSAVDGVSFDLAEDDILCLLGPSGCGKT
                     TLLRLIAGLETPDRGRVRFDGRDLAGVPPHTRDFGMMFQDFALFPHKNVFDNVAFGLR
                     MRHRPAEEIATRVTAMLTTVDLAGFEHREIGQLSGGEQQRVALARALAPGPRLLMLDE
                     PMGALDRALRERLMLDVRAILKRVGMTAVYVTHDQAEAFAVADHLAVMNAGRIEQFGR
                     PPAVYNHPATPFVARFLGFENLLPGQVDARGLVVTAAGRFRPPSPARGPAVTLLVRPA
                     LDQAAQPSGDDGGDVNVIDGLIDAVAFRGRFCQVWLIAGGERLLFEVMGDYDHGPGER
                     VRVPVSPQQLQVYEDAV"
     gene            complement(3600..5294)
                     /locus_tag="PK829_03440"
     CDS             complement(3600..5294)
                     /locus_tag="PK829_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU40302.1"
                     /translation="MRSLLLAAVFLSPLLFFALFYFYPLAAILRLGLWLEGRLDTVAL
                     GELFRSAYFLRVLWFTVWQAVVSTLLTLALALPGAYVLARYRFRGQTLIRAVATLPFV
                     LPTVVVAAAFVALVGPAGVLNVWLMRLLALDAPPIRLSQTVWIILLAHVFYNYSVALR
                     LLSAYWQNLPPSLSQAAQMLGASPARAFLTVTLPLLRPALLAAAALVFTFTFTSFGVI
                     VVLGGPRFATLEVEIYRQAVNLFNLPVAAALSLWQILFTFILMAGYTRTQLQVTRALR
                     LGARRSIEQPVRTPRQRLLVIGNAAAILVLIGAPLLALVSRSLRGAAGPTLAYYRALF
                     TNRDDSLFFVPPVVAIRNSIAVALVATLLAVALGLITAQLLTRWERGPGRGNGGRVAL
                     ARLLDPLLMLPLATSAVTLGLGYLIALGRPPLNLRGSILILPIAHAVVAAPFVLRSVL
                     PGFRSILPSLREAAAVMGADGWRVWREVDWPLIRRPLLVGALFAFTISMGEFGATIFL
                     VRPETPTLPIAIYRFLGQPGALNYGQALAMSVLLLLVCAVAFVAIEHFRVGGEGGF"
     gene            5474..5980
                     /locus_tag="PK829_03445"
     CDS             5474..5980
                     /locus_tag="PK829_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOU40303.1"
                     /translation="MADSLREYHAKRDFAQTAEPSGDDTPRRRGDEPIFVIQRHDAST
                     LHYDFRLESDGVLVSWAVPKGLPQKTGTRRLAVRTEDHPLAYATFEGRIPKGQYGAGQ
                     VEIWDKGTYRNLRADKEEAERASMPEAIAEGKIEVYLDGSQISGSYAIIRTDSDEGNW
                     IILRMKGD"
     gene            5986..6897
                     /gene="ligD"
                     /locus_tag="PK829_03450"
     CDS             5986..6897
                     /gene="ligD"
                     /locus_tag="PK829_03450"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOU40304.1"
                     /translation="MTTKSFTTDGRTIEVSNANKVLFPDIGLTEGQLAEYYAQVAGTM
                     LPHLRGRPLSMQRFPKGIGERGFYAKEMPDYFPEWIDHVDVDVLETGEVQPQVVCNDA
                     ATLIYLVDQACITPHTWLSRRPHLNQPDKLIFDLDPPRSFETAREAARSLRTLLDELE
                     LPAYLMTTGSRGLHIGIPLDGQAGFDAVRAFAVELAEALARRAPDKLTTETRKNARRG
                     RLFLDYLRNSYAQTSVPPYAVRARPGAPVATPIRWEELDDPELTSSRYTVANVLNKIA
                     REGDPWADFHERGCSLTAVRARLGALR"
     gene            complement(6894..7523)
                     /locus_tag="PK829_03455"
     CDS             complement(6894..7523)
                     /locus_tag="PK829_03455"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU40305.1"
                     /translation="MATEAERAEQLAAIREEIAGLTESPLYDYRTTNHYNPAIGEGNP
                     MAEIVLIGEAPGEVEARTGRPFVGPAGRVLDDLLASIDLKREDIYITNVVKDRPPDNR
                     DPRVKEVRLYSPFLWRQLEIIQPKVIVPLGRHALDFVRKHFGGFPAEGSVSTLHGTAY
                     PVDAPYGRAFVVPLYHPAVLFYREDLKETVEADFRLLGELLKSRETTQS"
     gene            complement(7629..8681)
                     /locus_tag="PK829_03460"
     CDS             complement(7629..8681)
                     /locus_tag="PK829_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU40306.1"
                     /translation="MQRLKGLTALALLLVVLLAACGGAAETEGTTVRLMTHDSFDISA
                     EVLAAFEAETGITVEVFKAGDGGEALNKAILSKDAPLADVFYGVDNTFLQRALDNDIF
                     LPYDSPALADVPATLQLDPQHRALPVNYGDVCLNVDVAWFDERGLPPPASLDALADPA
                     YAGLTVVENPATSTPGLAFLLATIETYGQDGYLGYWQRLVDNNVLVANGWEEAYYTHF
                     SAASEGDRPIVVSYASSPVAEVYFAETPPETAPTAAVVADGACFRQIEFVGILSGTAV
                     EDAARQVVDFMLSKPFQEDIPLKMFVYPANEKAVLPEVFTQHSHIPAAPVQMDPARIA
                     ANREAWIQAWTETVLR"
     regulatory      complement(8755..8858)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1753 a         3021 c         2938 g         1409 t
ORIGIN      
        1 cggcggatgt cgatgccggg cacgtcgtta cccttataat aggtaacgat ggccacctcg
       61 acgcccctgg cctgtagcgc ccgcgtctcc tccaggatac ggatgtgccc cccgtagtca
      121 ctgaagaaag acgttggcgc gatcatcaac acgcgcaaac cgggctgccc cctgttctat
      181 ggtctaacgc cgctcgtctc tctggctggg cttcagcttg ttgctcagcc agccacccaa
      241 cacctgttcg cccaacgacg gccgctccac gggcgagtcc gctacctcga cccactcgtt
      301 ggcctcgcgc tcgcgcatcc agcgctcgcg ctgcaccagc agcccggcca gcgtcaggtc
      361 gatggcgtcg cggtcgccct cctgaatgag ccggcgaatg agcgtcagtt cgttcagcat
      421 cgcgtcaagc cagcgcagaa tagcggcgcg gtcgttcagg gccatcgccg taatatcagc
      481 gccgtgcagc agtggctggg tggccacggc aaaggaggga ttggcaaagc gcaggatgtc
      541 gcgccaggca gccgacttct gcaccgcgcc aaagagcgcc gcggccacca gaccaggcaa
      601 cgtttccacc gcctgggcca ggctgtcata ctccagcgga tcgacgaagt agggcaacgc
      661 ccccagcagg cggccgaagt tcaccgccgt gtcgaccgcg ccgggcgcgg ctttggccga
      721 gggcaagaga cagaagatgc tgttgcggaa cagatcgggc gtggccgcgg ctggctcgtc
      781 gcggccgtcg ttcaggtagg ccgccgacag cacgggcacg accccaatat agtggccgct
      841 ccgcagatgc ttatcggccc agcgcaggct ctgggtcttc aacggggcca cgtcgagcac
      901 cagcgcgtgc ggctgcacgt cctcgctgat gaccattaac gtgttttcca gttccgccag
      961 cggcacggcc agcaccagga tgtcggctgc cattgccgcg gcgacgaggt tccactccac
     1021 ccggtcgaca gccgcccact tatctttggc gtcctcggcc agttcgcgcg accggtcgtg
     1081 gccgacaatg gtcaggccag catcggccgc tcgcagcgcc tggccaatgg acacgccggt
     1141 ccggcccatc cccacaatcg tcaccgtctg tgccttcatg tcgtgtctcc tctagttctg
     1201 atcgtcagcc cattgcaggg cgcgcaggac atcgtcaccc tcgccgggcc tgtcggcgtg
     1261 gcgacgaatg agatcgacca cggcggcgcg cgtgccggcc gccgcggcca tcgccgcgcc
     1321 ccactccgga tggcgggcgc gaatgacgaa cggccgccgc cagccatcgg gcggcccctg
     1381 gccccagcgc gccgcgcgcc gcggcaacag tgccacgcca acgacaatca ccgtccggtc
     1441 ccaggccgtc aacggatagc gcgtcttgcc aacgtcgtgc agcagggccg cggccagcaa
     1501 atcgggatca ttatagcccg ccgcgcgcag gtcgcctaag acacggacgc tgtgccattg
     1561 gtcggccaac gacaggcgcg tgaatagcgc ccgctcggcc ggcgatagca ggacggcaac
     1621 ctcctgccgg gccacctcgc tcagcgggcc agcggtcagg ttgttcttca gttggcgcag
     1681 gcgatagcgg agacccacgt cagagggaac cacccccgat gagcagttgc agtaaagccg
     1741 cagccggcag accaacgaag aggttcaaca ctgccggcac aaagaagaca acggccatga
     1801 gaaggataaa gccgaactgc tccagcgggc gcagccgata gaccagctcg ctcggcaaca
     1861 gcgcatagag aatcttggag ccgtccagcg gcggaatggg gatgaggttg aaaaaggcca
     1921 ggatcaggtt gatccagatg aactccgaca gcagcaggga cggggagagc gcgctggagg
     1981 tctgagtgaa ggacaccacc cccaggcgga aaaagacggc ggcaatggca gccatcagca
     2041 aattggagac agggccggcg gcagcgacga tagccatcga ggtgcgcggg ttgccgcgca
     2101 tgttcatcgg attgaccatg actggcttag cccagccgaa gccggcgacg atgagcagca
     2161 gggtgccaat agggtccaga tgcttaagcg gattgagcgt gattcgaccc atgcggcgcg
     2221 gtgtcgggtc gcccagatag tcggccgtga cggcatgcga cagttcgtga atggtgaaag
     2281 cgataatcag gacaacgata cgggcgatga tggtgctgac atcgggcaaa ttgagcatga
     2341 tgtttctctt ggccaggctc cagggcactc tggctacagg ttaagtatac cggctatacg
     2401 caccacacgc gagattgatg cgcccccatg tagagacggg ccggtggccc gtctagcccg
     2461 tcggcccatc ccccatctgg cccatccccc gtctggcctg gcgacccacc tatcccaccc
     2521 agacccatca cgagatcagt tacactgcgt cctcatagac ttgtagctgc tgcggcgaaa
     2581 ccggaacgcg cacgcgctcg ccagggccgt ggtcgtagtc gcccatcact tcaaacagca
     2641 gccgctctcc tccggcaatc agccacacct ggcaaaagcg cccgcgaaag gcgacagcgt
     2701 cgataagccc gtcaatgacg ttgacgtcgc caccgtcgtc gccagacggc tgagctgcct
     2761 ggtcgagcgc cggtctgaca agcaaggtca cggccgggcc gcgggcgggc gacggcggcc
     2821 ggaagcggcc ggccgcggtg acaaccaacc cgcgcgcgtc cacctggccc ggcagcaggt
     2881 tctcgaagcc caggaagcgg gccacaaagg gtgtcgccgg gtggttatag acggccggcg
     2941 gccggccgaa ctgctcaatg cgcccggcgt tcatgaccgc caggtggtcg gccacggcga
     3001 aggcctcggc ctggtcgtgg gtgacgtaga cggcggtcat gccgacgcgc ttcaggatag
     3061 cgcgcacgtc cagcatcagc cgttcgcgca gcgcccggtc gagcgcgccc atcggctcat
     3121 cgagcatcag caggcgcggc cccggcgcca gcgcccgcgc cagcgccacc cgctgctgct
     3181 cgccgccgga gagttggccg atctcccggt gctcaaagcc ggccagatcg accgtggtca
     3241 gcatggccgt gacgcgggtg gcgatttcct cggccggccg gtggcgcatc cgcagcccaa
     3301 aggcaacgtt gtcgaagacg tttttgtgcg ggaagagggc gaagtcctgg aacatcatgc
     3361 cgaagtcgcg cgtatggggc ggcacgccgg ccaggtcgcg gccgtcgaag cgcactctgc
     3421 cgcggtcggg cgtctccagc ccggcgatga ggcgcagcag cgtcgtcttg ccgcagcccg
     3481 acgggccgag caggcaaaga atgtcgtcct cggccaggtc aaagctaacg ccatccacgg
     3541 cgctggtccc cggcgcaaac gacttggcta cgtcgcgcac ttccaatagg gccatcgggc
     3601 tagaatcccc cctcgccgcc gacgcggaag tgctcgatgg ctacgaaggc cacggcgcag
     3661 accagcagca gcaagacgct catggccagc gcctggccat aattgagcgc gcccggctgg
     3721 cccaggaagc ggtagatggc gatgggcagc gtcggtgttt ctggccggac aaggaagatg
     3781 gtcgccccga actcgcccat gctgatggtg aaggcgaaca gcgccccgac cagcagcggc
     3841 cggcgaatga gcggccaatc gacctcgcgc cagacgcgcc agccgtcggc ccccatgacc
     3901 gccgcggcct cgcgcagcga cggcaggatg ctgcggaagc cgggcaggac actgcgcaac
     3961 acaaacggcg cggcgacgac agcatgggcg atgggcaata tcaggatcga cccgcgcagg
     4021 ttgagtggcg gccggccgag ggcaatcagg tagcccagcc ccagcgtcac ggccgaggtg
     4081 gccagcggca acatcagcag cgggtcgagc aggcgggcca gagcaacgcg gccgccgttg
     4141 ccgcgccccg gcccgcgctc ccagcgtgtc agcaattgcg ccgtaatcag ccccagagcc
     4201 acagccagca gcgtcgccac cagggcgacg gcgatcgaat tgcgaatggc gaccacgggc
     4261 ggcacgaaaa agagcgagtc gtcgcgattg gtgaacaggg cgcgataata ggccagcgtt
     4321 ggcccggctg ccccgcgcag cgagcggctg accagcgcca gcagcggcgc gccgatgagc
     4381 accagaatgg ccgcggcgtt gccgatgacc aacaggcgtt ggcgtggcgt gcgcactggc
     4441 tgctcgatgc ttcgccgcgc gcccagccgc agggcgcgcg tcacttgcaa ttgcgtgcgc
     4501 gtatagccgg ccatcaggat gaatgtgaac aggatttgcc acagcgatag ggccgcggcc
     4561 acgggcaggt tgaacaggtt gaccgcctga cggtagattt cgacctccag cgtggcaaag
     4621 cgcggcccac ccagcaccac gatgacgccg aagctggtga aggtgaaggt gaaaacgagc
     4681 gccgccgcgg ccagcagtgc cgggcgcagc agcggcaacg tcaccgtcag gaaggcgcgc
     4741 gccggcgacg cccccaacat ctgcgccgcc tgcgacagcg acggcggcaa gttctgccag
     4801 taggcgctca gcaggcgcag ggcaacgctg tagttataga aaacgtgggc cagcaggatg
     4861 atccagaccg tttgcgacag gcgaatgggc ggcgcgtcca acgccagcag gcgcatcagc
     4921 cagacgttga gcacgcccgc cggcccaacc agggccacga acgccgccgc gacgacgacc
     4981 gtcggcagca cgaaaggcag cgtagccacg gcgcggatga gcgtttggcc gcgaaagcgg
     5041 tagcgagcca gcacgtatgc ccccggcagg gccagcgcca gtgtcagcag cgtcgagacg
     5101 accgcctgcc agacggtgaa ccatagcacg cgcaggaaat aggccgagcg gaataactct
     5161 cccagcgcga ccgtatccaa ccgcccctcc agccaaagac ccaggcgcag gatagctgcc
     5221 agcggataga agtagaacag ggcgaagaag agtagcggcg acaggaacac ggccgccaga
     5281 agtagcgaac gcacacggcg gcggaggctt ggaagagttg tcacctggtg catcattggt
     5341 cgcgccggag tgtaccccaa cttgtatcca gtgctctaac tgttatccct gcacagatat
     5401 gccccagagg attatgatga ctgtagacac atttagccga cagggtgact agaggaaagg
     5461 agtgactgtg accatggccg attcactacg cgaataccat gccaaacgcg acttcgccca
     5521 gacggccgag ccaagtggcg acgacacccc acgccggcgg ggcgatgagc ctatcttcgt
     5581 tatccaacgc cacgacgcca gcacgttgca ctatgacttc cgcctggaga gcgacggcgt
     5641 gctcgtctcc tgggccgtgc ccaagggact gccccagaag acgggcacgc gccgcctcgc
     5701 cgtccggaca gaagatcacc ccctggccta tgccactttc gaggggcgca tccccaaagg
     5761 gcagtacggc gcgggccagg tagagatttg ggacaagggc acctaccgca acctgcgcgc
     5821 cgacaaggag gaagccgaac gcgcctccat gcccgaggcc atcgccgagg gcaagattga
     5881 ggtctatttg gacggcagcc agatcagcgg ttcctacgcc atcattcgca ccgacagcga
     5941 tgagggtaac tggatcattc tgcggatgaa gggcgactaa cgcctatgac gaccaaatca
     6001 ttcaccaccg acggccgcac cattgaagtc tccaacgcca ataaggtgct cttccccgac
     6061 atcgggctga ctgagggcca gctggccgag tattatgccc aggtcgccgg aactatgctg
     6121 ccccatttgc gcggccggcc gctgagtatg caacgctttc ctaagggcat cggcgagcgt
     6181 ggtttctatg cgaaggaaat gcccgactac ttccccgagt ggattgacca cgtcgacgtc
     6241 gacgtgctgg aaacgggcga agtgcagccg caagtggtct gcaacgacgc ggccacgctt
     6301 atctacctcg tcgatcaagc ctgcatcacc ccgcacacct ggctcagccg caggccacat
     6361 ctaaaccagc ccgacaagct catcttcgat cttgacccgc cccggtcgtt tgaaaccgcg
     6421 cgcgaggctg ctcgctctct gcgaacgctg ctcgacgagc tagaactgcc tgcctatctg
     6481 atgaccaccg gttcgcgcgg gctgcacatc ggcatccccc tcgatggcca ggccggcttc
     6541 gacgccgtgc gcgccttcgc cgtcgaactg gccgaggcgc tggcccgtcg cgcgcccgac
     6601 aagctgacga ctgagacgcg caaaaacgcg cgccgcggcc gtcttttcct tgactacctg
     6661 cgcaacagct acgcccagac gtcggtgccg ccctatgccg tccgcgcccg gcccggcgcg
     6721 cctgtggcta cgcccattcg ctgggaggag ttagacgacc cggaactgac ttcgtcgcgt
     6781 tacacagtcg ccaacgtgct caacaagatc gcccgcgagg gcgacccctg ggcggacttc
     6841 cacgagcggg gttgctcgct gacggccgtg cgggcgcggt tgggcgctct gcgctaagac
     6901 tgggtcgtct cgcggctttt gagcagttcc cccagcagcc ggaagtcggc ttcgaccgtc
     6961 tctttcaggt cttcgcggta gaacagcacc gccgggtgat agagcggcac gacaaaagcg
     7021 cggccatagg gcgcgtccac cgggtaagcg gtgccatgca gagtcgagac ggagccttcg
     7081 gccggaaagc ccccaaagtg cttgcggacg aagtcgagcg catggcggcc caggggcaca
     7141 atcaccttcg gctggatgat ctccaactgc cgccacagga agggcgaata gagacgcact
     7201 tccttcaccc gcgggtcgcg gttgtcgggc ggccgatcct tgacgacgtt ggtgatgtag
     7261 atatcctcgc gcttgaggtc aatcgaggcc agcaggtcat ccagcacccg ccccgccggg
     7321 ccgacgaatg gccggccagt cctggcctcc acctcccccg gcgcttcgcc gatcaagaca
     7381 atttccgcca tagggttgcc ctcgccgatg gccgggttgt agtggttggt cgtgcggtag
     7441 tcatatagcg gcgactctgt caggccggcg atctcctcgc gaatagccgc cagttgctcg
     7501 gcgcgttctg cttcagttgc cattgctcaa ctccatcatg gtctaactcc taccataaca
     7561 cagagtcggc gccggcgaat gtaccaggat ataaagccac ggatacattt cccgccttct
     7621 catgctcctc aacgcagcac tgtctccgtc caggcttgaa tccacgcctc gcggttggcg
     7681 gcgatgcggg ccgggtccat ttgcacgggt gcggccggga tgtggctgtg ctgcgtgaac
     7741 acttctggca gcacggcctt ctcgttggcc ggataaacga acatcttgag ggggatgtcc
     7801 tcctggaagg gcttgcttaa cataaagtca accacttgcc gggcggcgtc ttccaccgct
     7861 gtgccgctga ggatgccgac aaactcgatc tgccggaagc aagcgccgtc ggcgacgaca
     7921 gcggccgtcg gcgcggtctc cggcggcgtc tcggcgaagt agacctcggc caccggactg
     7981 ctggcgtagc tgacgacgat gggccggtcg ccctccgagg cggcgctgaa gtgagtgtag
     8041 taggcctcct cccagccgtt ggcgacgagc acgttgttgt ctaccagccg ctgccagtag
     8101 ccgaggtagc catcctgacc atacgtctcg atggtcgcca gcaagaaagc caggcccggc
     8161 gtggacgtgg ccggattctc gacgacggtc aggccagcat aggccgggtc ggccagcgca
     8221 tccaggctgg ctggcggtgg caacccccgt tcatcgaacc aggccacgtc tacgttcagg
     8281 cagacgtcgc catagttgac cggcagggcg cgatgctgcg ggtccagttg cagcgtggcg
     8341 gggacgtcgg ccagcgcggg ggagtcatag ggcagaaaga tgtcgttgtc cagcgcccgt
     8401 tgcaggaagg tgttatcgac gccatagaag acgtcggcca ggggcgcgtc cttcgacagg
     8461 atggccttgt tgagcgcctc gccgccgtcg ccggctttga acacctccac ggtgatgccc
     8521 gtctccgcct cgaacgcggc cagcacttcg gcgctgatgt cgaagctgtc gtgggtcatc
     8581 aagcggacag tcgtgccctc cgtctcggcc gcgccgccac aggcggccaa caacacaacc
     8641 aacaggagcg ccagagccgt aagtcctttc aagcgttgca tggtcttcct ctcctcaggg
     8701 gcgcagggcg ggcacgccgc ccccaacgaa acgcgcccct aagaccaggg gcgcgaaacg
     8761 atttctgtca tttccctacg ccagcattac ctggatcagg ttcggcggtt cgctcaaagc
     8821 gtctcagccc ggtcacccgt gcacccgcaa gactgcgatt cagttgtcgc tgtcgggcgt
     8881 cttgcctaac aaccagcggg attataggcc ggtgccgggc gatcgtcaat cggggctttg
     8941 ccaaagtcgt cgagcgacca ttgacctgac aggtaggcgg ccagtcgttc tgtggtggca
     9001 gtcccatgcg tggtcgccca cctgtcaggt cggcgcgccg ggagtgactc tggcaaagtc
     9061 ctggatcgtc aatcgcagtt gacaaaggcg gccgtcgtgc tataatccgt tcttgtcgtt
     9121 g
//
LOCUS       DAOSMQ010000064         9079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7619_length_9079_cov_4.999889, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000064 DAOSMQ010000000
VERSION     DAOSMQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7619_length_9079_cov_4.999889"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1748
                     /locus_tag="PK829_03465"
     CDS             51..1748
                     /locus_tag="PK829_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU40307.1"
                     /translation="MHFQPFNQPAPRLGNQYDADRVLRAYLKRRLPADVLAEVEPALR
                     EMGEMSGGALYELQLADRLNEPALTQWDAWGERVDRIVVTPLWQRAERLAAEFGLVAT
                     AYERRYGPLSRAVQFALAYLFHPSTDVYTCPLAMTDGAARTLQLSGNRALIDRALPHL
                     TSRDPAAFWTSGQWMTESTGGSDVGRSETIACRDAGGWRLYGRKWFTSATTSPMTLTL
                     ARPQGNGPGGRGLALFYLELRDEDGRLNAISVNRLKDKLGTRKVPTAELLLDGAPATP
                     VMGLDNGVRNIIPMLHLTRTWNSVMAAATMRRGLALARDYAQRREAFGALLARQPLHI
                     DTLARLQAETEAAFHLSFFLVELIGADEAGDIAPAEADLLRLLTSIAKLTTGRQAVEV
                     ASEVLEAHGGAGYVEDTGLPLLLRDSQVLPIWEGTTNVLALDALRALAHGDTGLQTLR
                     RKVSDIAADARDAALVGAARQAEAAVDHALSWVRDAAAANRTVLEAGARRFGLTLGRA
                     LALALLVEAAEWSLAHERDGRARAAALRFARAPIDHIAVTDPRLDAALANDEPLSLA"
     gene            1763..3091
                     /locus_tag="PK829_03470"
     CDS             1763..3091
                     /locus_tag="PK829_03470"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU40308.1"
                     /translation="MSENNDLTFLDDLGLGFAIDRLGPATRPTPMRVSCLTSDDERIL
                     YHSDPTVVRRLIDEGRELPSFERAGPRERLFFDPRGLRAGIVTCGGLCPGINDVIRAI
                     VLSLHFHYGVEAIDGFRYGYEGLIPGPDHEPVALTPARVEMISDFGGTVLGTSRGDQD
                     VSRMVDTLVTRGVSILFTIGGDGTLRGAAAIAAEIARRHLPIAVVGVPKTIDNDIAFI
                     ERSFGFVTATAEARRVIEGAHSEALATRNGIGLVKLMGRESGFIAAHSSLADSRVNFC
                     LVPEVPFVLEGLLAALRDRLAERGHAVIVVAEGAGQELVGRSNARDASGNVKLGDIGL
                     FLREAINATFKELGPPVNLKYIDPSYTIRSLPANTYDSAYGLLLGYNAVHAAMSGRTN
                     MVVGYWNNEYTHVPIPLAVHRRKTIDPQGWLWNNVLLATGQRLASPAQRA"
     gene            3139..3495
                     /locus_tag="PK829_03475"
     CDS             3139..3495
                     /locus_tag="PK829_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40309.1"
                     /translation="MDTSSNLSMFLIGLAIGAAVALLGGVVDFALHLRGRREPSFGVP
                     GCLVYTAGGLILAGVIALIVSFVATGSLAPALAMGGGVLLGFYGGFILLVALWFVRDR
                     GPARAEEQLTSDSSLP"
     gene            3519..3854
                     /locus_tag="PK829_03480"
     CDS             3519..3854
                     /locus_tag="PK829_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40310.1"
                     /translation="MTIRIVITLALIAVGIGVMYALQGAYGGLSAVMVGSLFFLFALS
                     VWTLPVAAFERGNKLDPMQKRIIGAFLGIGLGMFGGLAVAALVPRPFNWIALGAIALG
                     ATIWWYRRS"
     gene            3873..5558
                     /locus_tag="PK829_03485"
     CDS             3873..5558
                     /locus_tag="PK829_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOU40311.1"
                     /translation="MTYNLASLDLPTLRGRTLRMFAAAIDSRPLSGALTGQLLKQGGI
                     IDLRAKTFSDAPTFYPLALAEAGTQRDAPPLDWDALQDRLLRRENREPFASVSDYARA
                     YRAGTTTPEAVAEMALAAVADADDDPRPLRAFRAIDRADVLKQARAATERLRAGRPLS
                     LLDGVPVAVKDEVDQVPYGTTVGTSFLGQTPATQDATVVARLRAAGALLLGKTNMHEI
                     GINPDGFNEHYGIIRNPYNLQCHAGGSSNGSAAAVAAGFCPVAIGADGGGSIRIPAAL
                     TGLVGLKATFGRVSERGAAPLTWSMGHLGPIGATVSDVALVYACIAGPDPDDPNSLHQ
                     PPVSLDQWDVADLRGVRLGIYAPWFEHADPQIVEDCRAIIERLVEMGATVHEVEIPEL
                     DEMRVAQALTILAEMAANMAAYEDHWAELSPSTRVNLTLGRAATAADYLQAQRVRTRA
                     IDHFRRALAICDVILTPATAVTAPVVPDNCEADGWSDLNTVTELMRFAMPANLTGLPA
                     IAFPVGYDPRGLPTAMQAMGRPWEEHLLLRVAYAAELVVARRCPPTFYHLLPH"
     gene            5573..5911
                     /locus_tag="PK829_03490"
     CDS             5573..5911
                     /locus_tag="PK829_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOU40312.1"
                     /translation="MDLTLRECQQQVDTWIRTVGVRYYSELTNLAVLVEEVGEVARLM
                     SRLYGDQSFKASDRGRELADEFADVLFVLICLANQTGVDLTAAFEATMVKKTARDAER
                     HANNPKLRGL"
     gene            6094..>7004
                     /locus_tag="PK829_03495"
     CDS             6094..>7004
                     /locus_tag="PK829_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009587042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU40313.1"
                     /translation="MTDLLRFDGRVAIVTGAGGGLGRAYALLLAARGASVVVNDLGGS
                     MHGEGAATTAADRVVDEIRAAGGTAVADYHSVEDGALIVQSALDAFGRLDIVINNAGI
                     LRDVSFHKMSAEDWDKIYRVHLYGGYQVTRAAWPILREQGYGRIVMTSSAAGLYGNFG
                     QANYSSAKLALLGLAQTLAVEGQKRNVLVNTIAPLAGSRLTETILPPELVAALRPEYV
                     APLVAYLCHESSSETGHTFEVGAGWVARVRWQRGRGAFFALTDALTPEAIAARWAEID
                     TFDDPTYPTTPTDSFAPVVANLQGSATA"
     assembly_gap    7005..7104
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7105..7983
                     /locus_tag="PK829_03500"
     CDS             <7105..7983
                     /locus_tag="PK829_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOU40314.1"
                     /translation="GPRDAIGYEFAPVTTSYDEKAVSLYALAVGAAADALDPAELPFV
                     YELSGDGFRALPTFGVTFPFALLWQITAVPGLRFNPALLLHGEQALQLRRPLPTKATV
                     THRAHIADIYDKGSAALVLLDVHSYDESGAELAYNRSSLFIRGLGGFGGERGPSAAAF
                     ALPQRPPDATRAQATTDNQALLYRLASGDGNPLHADPAFAAAGGFARPILHGLCTFAF
                     AGRAVLRDFAGNDPARIAGIKARFARHVFPGETIITAMWREQKEEDGMTRVLFQSKVA
                     ERDEIVLSNGVVDLTD"
     gene            complement(7993..>9079)
                     /locus_tag="PK829_03505"
     CDS             complement(7993..>9079)
                     /locus_tag="PK829_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOU40315.1"
                     /translation="TPDATPSDVATPESAPADADATGFTRQVVATNVKKTHATAAADY
                     DSDGDIDFAVTDYVNNLVLYYRNKGGNQYERTVLDGNLKGAYPIGLGDVNKDGKMDIV
                     AAGYLADTFVWYRNDGKGNFTRVIIDTHANGAHSIVVVDLDKDGDNDLVTAWQDGNTI
                     AWYENTGGNKFTRRIVDTKSPKAKRAAVADINGDGDLDIVSASFGGQVTWFENNGNEQ
                     FTKHVIDAAARGAYYVSPADVDKDGDMDIYAAIRRESAIVWYRNDGGGKFSYHVIDNK
                     AAGARTVLGVDFDKDGDMDALAASVDNDSVIWHRNDGKGNFTRVVLDTKIDGPYGLEV
                     VDINKDNKLDLLVAANMAGEIVLYRRN"
BASE COUNT         1385 a         3182 c         2905 g         1507 t
ORIGIN      
        1 ctccacgcct accgggcgca ggagccatag tctgaattgt ggagggcctg atgcacttcc
       61 aaccttttaa ccagcccgcc ccccgcctgg gcaaccagta cgacgccgac cgcgtgctgc
      121 gcgcctatct gaagcgccgc ctgccggccg atgtgctggc cgaggtcgag ccggcgctgc
      181 gcgagatggg cgagatgagc ggcggcgcgc tgtacgagct gcaactggcc gaccggctga
      241 acgagccggc gctgacgcag tgggacgcct ggggcgagcg cgtcgatcgc atcgtcgtca
      301 cgccgctgtg gcagcgggcc gagcggctgg cggccgagtt cggcctggtg gccacggcct
      361 acgagcgccg ctacggcccg ctctcccgcg ccgtccaatt cgccctggcc tacctttttc
      421 acccctcgac cgacgtctac acctgcccgc tggccatgac cgatggcgcg gcgcgcacgc
      481 tgcaactcag tggcaaccgc gccctcatcg accgcgccct gccccacctg accagccgcg
      541 acccggccgc gttctggacg agcggccagt ggatgaccga atcgaccggc ggcagcgacg
      601 tgggccgctc ggagactatc gcctgccgcg acgccggcgg ttggcggctc tatggccgca
      661 agtggttcac ctcggccacg acttcgccga tgacgctgac gctggcccgg ccgcagggca
      721 acgggccggg cgggcgcggg ctggcgctgt tctacctgga actgcgcgac gaggacgggc
      781 ggctgaacgc cattagcgtc aaccggctga aggacaagct gggcacgcgc aaggtgccga
      841 cggccgaact gctgctcgac ggcgcgccgg ccacgccggt catgggcctc gacaacggcg
      901 tgcgcaacat catccccatg ctccacctga cgcgcacctg gaatagcgtg atggccgcgg
      961 cgacgatgcg gcgcggtctg gctttggcgc gagactatgc gcaacgccgc gaagccttcg
     1021 gcgcgctcct cgctcgccag ccgcttcaca tcgacaccct ggcccgctta caggccgaga
     1081 ccgaggccgc cttccacctg agcttcttcc tcgtcgaact catcggcgcg gacgaagccg
     1141 gcgatatcgc cccggccgaa gccgatctgc tgcgcctgct gacctccatc gccaagctga
     1201 ccaccgggcg gcaggccgtc gaagtggcca gcgaggtgct ggaggcccac ggcggcgcgg
     1261 gctacgtcga ggacaccggt ctgccgctgc tgttgcgcga cagtcaggtg ctgcccatct
     1321 gggagggcac aaccaacgtc ctggccctcg acgcgctgcg ggcgctggcc cacggcgaca
     1381 ccggcctgca gacgctgcgt cgcaaagtca gcgacattgc cgcggacgcg cgcgacgccg
     1441 cgttggtcgg ggccgcccgc caggccgagg ccgctgtcga tcacgccctg agctgggtgc
     1501 gcgacgccgc cgcggccaac cgcacagtgc tggaggccgg cgcgcgccgt tttggcctga
     1561 cgctcggccg ggcgctggct ctggcgctgc tggtcgaggc ggccgagtgg tcgctggccc
     1621 atgagcgcga cggccgcgcc cgggccgcgg ccctgcgctt cgcccgcgcg cccattgacc
     1681 acatcgccgt gaccgacccg cgcctcgacg cggccctggc caacgacgag ccgctatccc
     1741 ttgcctaggg agtcaatgcc acgtgtcaga aaacaacgac ctcacctttc tggatgacct
     1801 cggcctgggc ttcgccatcg accggcttgg cccggcgacg cggcccacgc ccatgcgcgt
     1861 gagctgcctg acgtccgacg acgagcgcat cctctaccac agcgacccaa ccgtcgtccg
     1921 ccgcctgatc gacgaggggc gcgaattgcc ttccttcgag cgcgccgggc cgcgcgagcg
     1981 gttgttcttc gacccgcgag gactgcgcgc gggcattgtc acctgtggcg gtctgtgccc
     2041 cggcatcaac gatgtcatcc gcgccattgt cctgagcctg cactttcact atggcgtcga
     2101 agcgattgac ggcttccgct atggctacga ggggttgatc cctggccccg accacgagcc
     2161 agtggccctg acgccggcgc gggtggaaat gatcagcgac tttggcggca cggtgctggg
     2221 cacgtcgcgc ggcgaccagg acgtcagccg gatggtcgat acgctcgtga cccgcggcgt
     2281 cagcatcctg ttcaccatcg gcggcgacgg cacattgcgc ggcgcggcag ccatcgccgc
     2341 cgagatcgcc cgtcgccacc tgcccatcgc cgtcgtcggt gtgcccaaga ccatcgacaa
     2401 cgacatcgcc tttatcgagc gctccttcgg cttcgtcacg gccacggccg aggcccggcg
     2461 cgtcatcgag ggagcgcata gcgaagcgct ggccacccgc aacggcatcg gcctggtgaa
     2521 actgatgggc cgcgaatcgg gcttcatcgc cgctcactcc tcgctggccg atagccgggt
     2581 caacttctgc ctagtgcccg aagtgccgtt cgtgctggag ggcttgctgg cggcgctgcg
     2641 cgaccggctg gccgagcgcg gccatgccgt catcgtcgtg gccgaggggg ccggccagga
     2701 gcttgtcggt cgcagcaacg cccgtgacgc ctccggcaac gtcaagctgg gcgacattgg
     2761 cctcttccta cgcgaggcca tcaatgccac cttcaaggaa ctgggcccgc cggtcaatct
     2821 caagtacatc gaccccagct acacgatccg cagcctgccg gccaacacct acgactccgc
     2881 ttatggcctg ctgttgggct ataacgccgt tcacgccgcc atgagcggcc gcaccaacat
     2941 ggtcgtcggc tattggaaca acgagtatac ccacgtgccc atccctctgg ccgtccatcg
     3001 gcgcaagaca atcgaccccc agggctggct gtggaacaac gtcttgctgg ccacggggca
     3061 gaggcttgca tcgccggccc agcgggctta gaatacgctt agcttccgct ttacaacccc
     3121 tgttcccaca aaggcaccat ggacactagc agtaacctgt cgatgttcct gatcggtctg
     3181 gccatcggcg cggccgtcgc tctgctgggt ggtgtggttg acttcgcgct ccacctgcgc
     3241 ggccggcgcg agccgagctt tggcgtgccc ggctgcctcg tctacaccgc cggcggtctg
     3301 attctggccg gcgtcatcgc ccttatcgtc tcgttcgtcg ccaccggtag cctggccccg
     3361 gcgctggcta tgggcggggg cgtcttgctg ggcttctatg gaggcttcat actgctggtc
     3421 gccctctggt ttgtgcgcga ccgtggcccc gcccgggcag aggagcagtt gacgtccgac
     3481 tcgtcgctgc catagcctgt ccccacccgg agccgcctat gaccattcgc atcgtcatca
     3541 cactggccct gatcgccgtc ggcatcggcg tcatgtacgc cctgcaaggg gcctacggcg
     3601 gtctatccgc cgtcatggtc ggctcgcttt tcttcctctt tgccctctcc gtctggacgc
     3661 tgcccgtggc cgccttcgag cgcggcaaca agcttgaccc gatgcagaag aggatcatcg
     3721 gcgcgtttct gggcattggt ctgggcatgt tcggcgggct ggccgtggcc gcgctcgtgc
     3781 cgcgtccgtt taactggatc gccctcggcg caatcgccct gggcgccacg atctggtggt
     3841 atcggcggtc ataagcaccc tggagcgaca ccatgaccta caacctcgcc agcctcgatc
     3901 tgcccaccct gcgcggacgc actctgcgga tgttcgctgc cgccattgac agccggccgc
     3961 tgagtggcgc tctgaccggc caactgctca agcagggcgg catcatcgat ctgcgcgcca
     4021 agaccttcag cgacgcgccg accttctacc cgctggctct ggccgaggcc gggacgcagc
     4081 gtgacgcgcc gccgctcgat tgggacgcgc tgcaagaccg gcttctgcgg cgcgaaaacc
     4141 gcgagccgtt cgccagtgtg tctgactacg cccgcgccta tcgcgccggc acgacgacac
     4201 ccgaagccgt ggccgagatg gcgctggcgg ccgtcgccga cgccgacgac gacccccggc
     4261 cgctgcgcgc cttccgggcc atcgaccgcg ccgacgtgct gaagcaagcc cgcgccgcca
     4321 ccgagcggct gcgcgccggc cggccgctga gcttgctcga cggcgtgccc gtggccgtca
     4381 aggacgaggt cgatcaggtt ccctatggca cgaccgtggg cacgagtttc ctgggccaga
     4441 cgccggcgac gcaagacgcc accgtcgtcg cccgactgcg cgccgccgga gccttgctgc
     4501 tcggcaaaac caacatgcac gagatcggca tcaaccccga cggcttcaac gagcattacg
     4561 gcatcatccg caacccctac aacctgcaat gccatgccgg cggcagctcc aacggctcgg
     4621 ccgcggccgt ggccgccggt ttctgcccgg tggccatcgg cgcggacggc ggcggctcca
     4681 ttcgcatccc cgcggcgctg accggcctgg tcggcctgaa ggcgacgttc ggccgcgtca
     4741 gcgagcgcgg cgcggcccca ctgacctggt cgatgggcca tctggggcca attggcgcga
     4801 cggtcagcga tgtcgccctg gtctacgcct gcatcgccgg gcctgatccc gacgacccca
     4861 acagcctgca ccaaccgccc gtcagcctcg accagtggga cgtggccgat ctgcgcggcg
     4921 tgcgcctggg catctacgca ccctggttcg agcacgccga cccgcagatc gtcgaggact
     4981 gtcgcgccat catcgagcgc ctggtggaaa tgggcgcaac ggtgcacgag gtcgagatcc
     5041 ccgagttgga cgagatgcgc gtggcccagg cgctgaccat cctggccgag atggccgcca
     5101 acatggccgc ctacgaagac cattgggccg agctaagccc ctccacgcgc gtcaacctga
     5161 ccctcggccg ggcggctacg gcggctgact acctccaagc ccagcgtgtg cgcacgcgag
     5221 ctatcgacca cttccgccgc gccctggcca tctgcgacgt catcctgacg ccggccacgg
     5281 ccgtcaccgc gcccgtcgtc cccgacaact gcgaggccga cggctggtcg gacctgaaca
     5341 cggtgacgga gctgatgcgc ttcgccatgc ccgccaacct gaccggtctg ccggccatcg
     5401 ccttcccggt tggctacgac ccgcgcggcc tgccgacggc catgcaggcg atgggccggc
     5461 cgtgggagga gcatctgctg ttgcgcgtgg cctacgccgc cgagcttgtc gtcgcccgcc
     5521 gctgcccgcc aacgttctat catctgctgc cccactaagc ccggagccgg acatggacct
     5581 gaccctcaga gaatgccagc agcaggtcga tacgtggatt cgcaccgtcg gcgtgcgcta
     5641 ttactcggaa ctgaccaatc tggctgtgct ggtcgaggaa gtgggcgagg tcgcccggct
     5701 gatgtcgcgc ctctatggcg accagagctt caaggcgtcc gaccgcggcc gcgaactggc
     5761 cgacgagttc gctgacgtcc tcttcgtcct catctgcctg gccaaccaga ccggcgtcga
     5821 cctgacagcc gccttcgagg cgacaatggt caagaagacg gcgcgagatg cggagcggca
     5881 cgcgaataac cccaaactgc ggggcctgta ggacgggttg gcaacccgtc tcttttccga
     5941 gcagggccaa agcctgtagc acgggttggc aacccgtctc tttttcgagc aaggccaaag
     6001 cctgtagcac gggttggcaa cccgtctctt ttccgagcag ggccaaagcc gacgggtcaa
     6061 cgacccgttc tacaaccagc aaggagtaat cgcatgacag acctactgcg ctttgacgga
     6121 cgggtggcca ttgtcaccgg cgcgggcggg gggctggggc gggcctatgc cctgctgctg
     6181 gccgcgcgcg gggccagtgt cgtcgtcaac gacctgggcg gcagcatgca cggcgagggc
     6241 gcggcgacga cggccgccga ccgcgtcgtg gacgaaatcc gcgccgcggg cggcacggcc
     6301 gtggccgact accattccgt tgaagacggc gcgctcatcg tccagagcgc tctcgatgcc
     6361 ttcggccgcc tcgacatcgt catcaacaat gccggcatcc tgcgcgatgt cagctttcac
     6421 aagatgagcg ctgaggactg ggacaaaatc taccgcgtcc acctctacgg tggctaccag
     6481 gtgacgcgcg ccgcctggcc catcctgcgc gagcagggct atggtcgcat cgtcatgacc
     6541 agctcggccg ccggcctcta cggcaatttc ggccaggcca actacagcag cgccaagctc
     6601 gccctgttgg gcctggcgca gacgctggcc gtggaaggcc agaagcgcaa cgtcctggtc
     6661 aacaccatcg ccccgctggc cgggtcgcgc ctgaccgaga cgatcctgcc gccggaactg
     6721 gtcgccgcgc tgcggccgga atacgtcgcc ccgctggtgg cctacctctg ccacgagagc
     6781 agcagtgaaa ccggccacac cttcgaggtc ggcgcgggct gggtggcgcg ggtgcgctgg
     6841 cagcgcgggc gcggcgcgtt cttcgccctg accgacgcgc tgacgccgga ggccatcgcc
     6901 gcgcgctggg cagagatcga taccttcgac gacccgacct acccgacgac gcccacagat
     6961 tcgttcgcgc ccgtcgtggc caacttgcag ggcagcgcga cggcnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnggcccg cgcgacgcca tcggttacga gttcgcgccg
     7141 gtgacgacca gctacgacga gaaggcggtc agcctctacg cgctggccgt cggcgcggcg
     7201 gccgacgcgc tcgacccggc cgagttgccc ttcgtctatg agctttccgg cgacggcttt
     7261 cgggcgctgc cgaccttcgg tgtcaccttc ccgttcgcct tgctgtggca aatcaccgcc
     7321 gtgccgggac tgcgcttcaa cccggcactg ctgctgcacg gcgagcaggc cttgcagctg
     7381 cgccggccgc tgcccacgaa agcgaccgtc acccaccgcg cccacatcgc cgacatctac
     7441 gacaaggggt cggccgcgct ggtgctgctc gatgtccata gctacgacga gagcggcgcg
     7501 gagttggcct ataaccgctc atcgctgttc attcgcgggc tgggcggctt cggcggcgag
     7561 cgcgggccgt cggcggcggc cttcgccctg ccccagcgcc cgcccgacgc cacgcgcgcc
     7621 caggccacga ccgataacca ggccctgctc tatcggctgg cctccggcga cggcaatccg
     7681 ctgcacgccg accccgcctt cgccgccgcc ggcggctttg cgcggcccat tctgcacggc
     7741 ctgtgtacct tcgcctttgc cgggcgggcc gtattgcgcg acttcgccgg caatgacccg
     7801 gcccgcatcg ccggcatcaa ggctcgcttt gcccgccacg tcttccccgg cgagaccatc
     7861 atcaccgcga tgtggcggga acaaaaagaa gaggacggca tgacgcgcgt cctcttccag
     7921 agtaaggtcg ccgaacggga cgagatcgtc ctgtcgaacg gcgtggtcga tctgaccgat
     7981 tagggcagcg ccctagttgc gacgatagag cacgatctcg ccggccatgt tcgcggccac
     8041 caacagatcg agcttgttgt ccttgttgat atcgacgacc tccagaccgt aggggccatc
     8101 aattttagtg tcaaggacaa cgcgggtaaa gttgcccttg ccgtcgttgc ggtgccagat
     8161 gacgctgtcg ttgtccacgc tggcggccag ggcgtccatg tcgccgtcct tgtcgaaatc
     8221 gacgccaaga acggtgcgcg cgccagcggc tttgttgtcg atcacgtgat aggagaactt
     8281 gccgccgccg tcgttgcgat accagacaat ggctgactcg cgccggatgg cggcgtagat
     8341 gtccatgtcg ccatctttgt cgacatcggc cggggagacg tagtaagcgc cacgtgccgc
     8401 cgcgtcgatg acgtgcttgg tgaattgctc gttgccgttg ttctcgaacc aggtcacctg
     8461 accgccgaaa ctggcgctga cgatgtcaag gtcgccatca ccatttatgt cggcgaccgc
     8521 ggcgcgcttg gctttgggcg actttgtgtc gacaatccgg cgtgtaaact tgttgccgcc
     8581 ggtgttctca taccaggcaa tcgtattgcc gtcctgccac gcggtcacca ggtcgttgtc
     8641 gccgtccttg tcgaggtcta cgacgacgat tgaatgcgcg ccattggcgt gggtatcgat
     8701 gatgacacgt gtgaagttgc ccttgccatc gttgcgatac cagacaaagg tgtcggccag
     8761 atagcccgcg gcgacaatgt ccatcttgcc atccttgttg acatctccca ggccgatcgg
     8821 gtaagcgccc ttgaggttac catccaggac ggtgcgctcg tactggttgc cgcccttatt
     8881 gcggtagtag agcacaaggt tgttgacata atccgtaacg gcaaagtcga tgtcgccgtc
     8941 actgtcgtag tcggccgcgg cggtggcatg ggtctttttg acgttggtgg ccacgacctg
     9001 cctggtgaaa ccggtggcat cggcgtcggc gggggcactc tccggcgtgg ctacgtcgga
     9061 cggggttgca tcgggcgtg
//
LOCUS       DAOSMQ010000065         8966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7749_length_8966_cov_4.099764, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000065 DAOSMQ010000000
VERSION     DAOSMQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7749_length_8966_cov_4.099764"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK829_03510"
     CDS             <1..288
                     /locus_tag="PK829_03510"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HOU40316.1"
                     /translation="AEEALRALSQDPSVQELLRLRQDAERTRATWREEALSEGRVEGQ
                     LELLARLIAKRFGGVAALYHDRLIKLSPEGLNSVTERLLTAESLEELLGAG"
     gene            601..1065
                     /locus_tag="PK829_03515"
     CDS             601..1065
                     /locus_tag="PK829_03515"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HOU40317.1"
                     /translation="MLRRRIPTVLIVLLALMLTLAIAVAAFAAPGDSLRRTTSLWGAN
                     EVPGPGDPDGSGEATIKINMSRHQLCYNLVVRRIAPATAAHIHVGDPTVAGPVVVPLG
                     APTDGHSSGCIQNVDPMLLRAILRSPRDYYVNVHNADYPSGALRGQLHHPGS"
     gene            1217..2554
                     /locus_tag="PK829_03520"
     CDS             1217..2554
                     /locus_tag="PK829_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU40318.1"
                     /translation="MNVKRRLLFLPFLLVMAACAPSGAADLTNPPAGPAPETAAAATE
                     GEIVQLVYQDWRTDWFPGMAQEQLAKFHATHPNIRVFYTPDPEDVPGEMPAMFQSGSA
                     PDVISGCCDFFPAWAQAGYLLDLAPYVQRDLSAETIAEWDEAQYQSFFTADGVQYALP
                     KYHGALAVYYNKDMFDAAGLAYPGADWTYDDYLADMTALTSADGRVWGSTLDPIYDRV
                     QIHVNAFGGHFVNPDDPTDCVLDEAPAVAALEWLRARFQDDGVMASPLALNKLETRRA
                     FWEGRVAMVEDGSWALKDILVNAPFRVGVATFPVGPARRATLATTDGFAIAANTQHPA
                     EAWELLKFLVGEEYGLAMARASFLQPARASLVDQWAELVRADLPQQTADLDLSVFADG
                     HTRGYSVTAEVFANMIGVRDITDAAFEDILSLGQAEPAARLAQACQEIEALQP"
     gene            2647..5412
                     /locus_tag="PK829_03525"
     CDS             2647..5412
                     /locus_tag="PK829_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU40319.1"
                     /translation="MTMRGSLRTKILTLALLPAVAMYLLVAVLSYFAAQAVAEDLVID
                     RDRELARLSAAELSASLQEYPELLSGVARDLAAAGDSEEALGSNLSGMANRLTYFDGG
                     VALLDNLGRVVATYPAAGTAGDDWSAQPIFRQIIQNPNRPSFSNLISNSLSGGEPALA
                     LAVPVRTARNELRGVLVGQFRVGPDVLNAFYGTLLRLRLDSKGQAYVIDAAGRLIFAT
                     PEDNAAAGRSLDDHPVVGPALSGGAGALRTTSAGKEALASYGPIPGTGWSLVIEEDWA
                     TLMTPLRRYAAGLSLLLLLGIIVPALVVAGGMQRITRPINALVEAARQVARGDFGRTV
                     SAPTGDEIETLAEQFNHMSAELQSSYAQLEQRVADRTRELQTVLEISRQVASTLDLSP
                     LLTQILDRLRAVVDFRFARLFIMEGDTPVLLEERGEDVDLADFYYLAGMPETEAMLAR
                     GEPLLLADTSQESPAVLHLRRVAIEQGNADIMDSIGSILSLPLMAREQRVGIMTLVHG
                     ERGYYTPTRVGVAMAFAAQAAVAVENARLFAAEAERITQLRVINQVSQSIAGILDLDG
                     LLRETAALIHERFGYYHVGIGLVEGDEVVYRAGAGQLVSPKGEILFSPSRLRVGKDGL
                     TGRAAGTGLVMISPDVSRDRRYVPLVGLDTRSEVVVPIKSKEVVIGVLDIQSETLNAF
                     DESDVDLLRALANQLAVAIENARLYEQAGQLAALEERQKLARELHDSVSQALYGIALG
                     ARTAHTVVDRTAAIDDDTRGKLTEPLDYVLAQANAGMAEMRALIFELRPESLQTEGLV
                     AALRKQTAALSARHQIPVTTEFGPEPNVPPAQKEMLYRVAQEAMHNIVKHARATAAEV
                     RLVSENGRVVMEVCDDGQGFDMSQEFPGHLGLKSMRERVERGGGLLTIASAPAGGTRV
                     MVKVSTE"
     gene            complement(5493..6023)
                     /locus_tag="PK829_03530"
     CDS             complement(5493..6023)
                     /locus_tag="PK829_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40320.1"
                     /translation="MRRGVVLPGSTLGEEESVATAAGRLSAEDVITLTQVAAWWRYRL
                     ERADASDPVASLIGGVDAKATTTTSDLGGGLLTLTPALFVPPNTGRALLLHAYGHSTN
                     LYPPSVYSLLAVAHCYNGPIGATVEANRVSYMPTGATSLNLQAALTPGTNALVLSIVG
                     LTGVTIEWMADVYWLG"
     gene            complement(6020..6736)
                     /locus_tag="PK829_03535"
     CDS             complement(6020..6736)
                     /locus_tag="PK829_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40321.1"
                     /translation="MSTHTGTGRRAEAAGSVAAAAVLANCRVALDDAAAAEFSDADLL
                     TFLNEAIREYSQHLPRVAELTLTALAGVRRYALPGDTWAILDVEYPEGTEPAVYLTRR
                     PYKGRAFAAALDAYDFLPRHDLTNPPALLLSFDPPTGATLRLRATRPHAALASVNDYL
                     TVPDAHHHVLLQYVLFAAARRLQAAEQAAPSAGSLLMAQLAANARRLELAYLNALNRI
                     LTQRTGESAVVSWGAAGGGR"
     assembly_gap    6779..6878
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6895..7848)
                     /locus_tag="PK829_03540"
     CDS             complement(6895..7848)
                     /locus_tag="PK829_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40322.1"
                     /translation="MFLKQSTSATLTLGPFVDDADGKTAETGLAIAQADVRLSKNGGA
                     YAQKNDATSPSHQENGFYACPLNTTDTSTLGRLRVAVSKTGALPVWQDFVVLPANTYD
                     SLVLGSDRLDVDAAQVTANAITSSALDASAAAELADAVWDEARAGHTSAGTFGLYLDA
                     QVSAAASPPSAATIADAVWDEALSSHLATGSAGDQLNNAGGAAADPWAAALPGTYPAN
                     TAGWIIGQRLDARVSAMSGNSPGAGAAEFTYTLTEASAGDPIADADVWVTTDSAGAVV
                     VASGRTDQNGRVTFYLDPGTVYLWRQKSGWNFANPDAETVG"
     gene            complement(7852..8424)
                     /locus_tag="PK829_03545"
     CDS             complement(7852..8424)
                     /locus_tag="PK829_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40323.1"
                     /translation="MTTLTRAVAASADDAVELSGVMTLNGVNLNANSAGQNIGLRFAN
                     VTIPAGATITAAYLTLNVVSTSYDDPNLTIRGSGEATTTTFTSTTNDITTRFKTAATV
                     TWNAGSIGAGARNSPDLSAILAEILAIPGWASGNAINFYLFPNAGSALRVYAYDNGSN
                     APQLTIDYTEPAAAGQPARSQHQFRLRRGL"
     gene            complement(8504..>8966)
                     /locus_tag="PK829_03550"
     CDS             complement(8504..>8966)
                     /locus_tag="PK829_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40324.1"
                     /translation="AAAWRAARAECFQHSEVGSGKPLGIFPRFLLVPAELYDTALTIL
                     GYGEGMPTVYTPEAVGRGFADPRPIPLVVPDWTDDNNWAYVVDPAVFPVIQMSYSQAP
                     GGRRHPIPELFAVVSETSGLMFSNDILPIKVRDEFALGVNGHRGIGKRNVS"
BASE COUNT         1311 a         2922 c         3170 g         1463 t
ORIGIN      
        1 gccgaagagg ccctgcgcgc actatcccag gatccgagcg ttcaagagct gctgcgtctg
       61 cgccaagacg ccgagcgcac ccgagcaacc tggcgcgaag aggccttgag cgagggtcgc
      121 gtcgaggggc agctcgagct tctggcgaga ctcatcgcga agaggtttgg tggagtcgct
      181 gccctgtatc acgaccgact catcaagctg agccctgagg ggctgaattc cgtgaccgag
      241 cggttgctga cggccgagtc tctcgaagag ctgctcgggg cggggtaggc cgacgctcgg
      301 cgaacaacta cctgtgcccg ctcgcgcccc gctcgcgccc cgcaaaggcc accgagatgc
      361 ccggcagcct gggtcattca gcagacgccg ccgcgtagat gtgaagttca tgggaaaggg
      421 aaatggacgg tcgtccacct gttgactagt ccattggtac tatgctatga tgctgctctc
      481 ttagcgggtc cagtcactcg caacacttca tcgccgcacc gacgtaagac aggcccgccg
      541 caataacggc taccggtgtt tgcgcggccc tacgggcgtt cgattgagga gagttgcgct
      601 gtgttgagac gacgtattcc caccgttctg attgtcctgc tggccctgat gctcaccctg
      661 gccattgccg tggcggcctt tgccgccccc ggcgactcac tgcgccggac gacctcgctc
      721 tggggggcca acgaagtccc cggccccggc gaccctgacg gcagcggcga ggcgaccatt
      781 aagatcaata tgagtcgcca ccagctgtgc tacaacctgg ttgtgcgccg catcgccccg
      841 gccacggccg cccacattca cgttggcgac ccgacggtcg ccggcccggt cgttgtgcct
      901 ctgggcgcgc cgaccgacgg ccactcctcc ggctgtatac agaatgtgga cccgatgctg
      961 ctgcgggcca tcctgcgtag cccgcgcgat tactacgtca acgtccacaa cgccgactac
     1021 ccgtccggcg ctctccgcgg ccagttgcac catcccggca gctgatcacg cacgaggcag
     1081 ggcctcgtga ctctacgtct ttgctgttcc acaaggggcg ggcctcagaa acccgcccct
     1141 ttgtcttttg tgcccgcttt ctggcgcgcg cggcgcggat tttccggccg gcgacgcgat
     1201 gcgtataata gtggccatga atgtgaaacg ccgcttgctt ttccttccct tcctgctggt
     1261 catggccgcg tgcgcgccca gcggcgccgc cgacctgacc aacccgccgg ctgggcccgc
     1321 cccggaaacg gctgcggccg cgaccgaggg cgagatcgtc cagctcgtct accaggattg
     1381 gcgcaccgac tggtttcccg gcatggccca ggaacagctg gccaagttcc acgccaccca
     1441 ccccaatatc cgcgtcttct acacgcccga ccccgaggac gtgcccggcg aaatgccggc
     1501 catgttccag tccggctccg cgcccgacgt gatttccggc tgctgtgact tcttccccgc
     1561 ctgggcgcag gccggctatc tgcttgacct ggccccctat gtccagcgcg acctctcggc
     1621 cgagaccatc gccgagtggg acgaggcgca gtaccaatcg tttttcaccg ccgatggcgt
     1681 gcaatacgcc ctgcccaagt accacggcgc attggcggtc tattacaaca aggacatgtt
     1741 cgatgccgcc ggcctggcct accccggcgc cgactggact tacgacgact acctggcgga
     1801 catgaccgcg ctgacgtcgg ccgacggccg cgtctggggc agcacgctcg accccatcta
     1861 tgaccgggtg cagattcacg tcaacgcctt tggcggccac ttcgtcaacc ccgacgaccc
     1921 gaccgactgc gtgctggacg aagccccggc cgtggccgcg ctggagtggc tgcgggcacg
     1981 cttccaggac gacggcgtga tggcctcgcc gctggcgctc aacaagctgg agacgcggcg
     2041 ggccttctgg gaagggcgcg tggccatggt cgaagacggc tcctgggcat tgaaagacat
     2101 cctcgtcaat gcccccttcc gcgtcggcgt ggccaccttc cccgtcggcc cggcgcgccg
     2161 ggcgacgctg gccaccactg acggcttcgc cattgccgcc aacacgcagc accccgccga
     2221 agcgtgggag ttgctgaagt ttctcgttgg cgaggagtat ggcctggcca tggcgcgggc
     2281 cagcttcctg caaccggcgc gcgcctcgct ggtagaccaa tgggccgagt tggtgcgcgc
     2341 cgatctgccg cagcagacag ccgaccttga cctgtctgtc ttcgccgacg gccacacgcg
     2401 cggctattcc gtcacggccg aggtctttgc caacatgatc ggcgtccgcg acatcaccga
     2461 cgccgccttt gaggacatcc tgtctctggg ccaggccgaa ccggcggcgc gattggcgca
     2521 ggcctgccag gagatcgagg ccttgcaacc gtagggacgc gcctgcgtgc gcgcccgcca
     2581 cgtgtgcgcc ggggttggcc agggccggca cgtagcccgg ccccaacggg cgggaacgga
     2641 cgaaacatga ccatgcgtgg ctcgctgcgg accaagattc taaccctggc gctgctgccg
     2701 gccgtggcga tgtacctgct tgtcgccgtg ctgagttact tcgccgcgca ggccgttgcc
     2761 gaagacctgg tcatcgaccg cgaccgggag ctggcccgcc tgtcggccgc cgaactgagc
     2821 gccagcctgc aagagtaccc ggagctgctg tccggcgtgg cccgcgacct ggccgccgcc
     2881 ggcgatagcg aggaagccct cggtagcaac ctgtcgggaa tggccaaccg gctcacctac
     2941 ttcgacggtg gcgtcgccct gctggacaac ctcggccggg ttgtggccac ttacccggcc
     3001 gccggcaccg ccggcgacga ctggtcggcc cagcccattt tccgccaaat catccagaac
     3061 cccaaccgcc cgtctttctc caatctgatc agcaacagtc tgagcggcgg cgaaccggcg
     3121 ctggccctgg ctgtaccagt gcgaacggcg cgcaacgaat tgcgcggcgt gctggtgggc
     3181 cagttccgcg tcgggccaga tgtgctcaac gccttctacg gcacgctcct gcgcctgcgg
     3241 ctggacagca aggggcaggc ctacgtcatt gacgcggccg ggcggctcat cttcgccacg
     3301 cctgaggaca acgccgccgc gggccggtcg ctcgacgatc atcctgtcgt cggcccggcg
     3361 ctgtcgggtg gcgccggcgc gttgcggacg acctcggccg gcaaggaggc gctggccagc
     3421 tatggcccca tccccggcac cggctggagc ctggtcatcg aagaggactg ggccacgctg
     3481 atgacgccgc tgcgacgcta tgccgccggg ctgtcgctgc tgctgttgtt gggcatcatc
     3541 gtgcccgcgc tggtcgtcgc cgggggcatg cagcgcatta cccggccgat taacgccctc
     3601 gtcgaggccg cgcgccaggt ggcccgcggc gacttcggcc gcaccgtcag cgcccccacg
     3661 ggcgacgaga tagagacgct ggccgagcag ttcaaccata tgtcggccga gttacagtcg
     3721 tcctatgctc agctggagca gcgcgtcgcc gaccgcaccc gcgaactgca aacggtgctg
     3781 gagatcagcc gccaggtggc ctcgacgctg gacctgtcgc cgctgctgac gcagatcctc
     3841 gaccggctgc gggcggtggt ggactttcgc ttcgcccggc ttttcattat ggaaggcgac
     3901 acgcccgtgc tgcttgagga gcgcggcgag gacgtcgacc tggccgattt ctactacctg
     3961 gccggcatgc cggagacgga agccatgctg gcccgcggcg agccgctgct cctggccgac
     4021 acaagccagg agtcgccggc cgtgcttcac ttgcggcgcg tggccatcga gcagggcaac
     4081 gccgacatca tggacagcat cggctccatc ctcagcctgc cgctgatggc ccgcgagcag
     4141 cgcgtgggca tcatgaccct cgtccacggc gagcgcggct actacacgcc gacgcgcgtg
     4201 ggcgtggcca tggccttcgc cgcccaggcg gccgtggccg tcgagaacgc ccgcctcttc
     4261 gccgctgagg ccgagcgcat cacccagctg cgggtcatca accaggtcag ccagtccatc
     4321 gccggcattc tcgaccttga cgggttgctg cgcgagacgg ccgcgctcat ccatgaacgc
     4381 tttggctact atcacgtcgg catcggcctg gtggagggag atgaggttgt ctaccgcgcc
     4441 ggggcgggcc aactggtgtc gccgaagggg gagattctct tcagccccag ccgcctgcgc
     4501 gtcggcaagg acggcctgac tggccgggcg gcgggcaccg gcctggtgat gatcagcccc
     4561 gacgtgagcc gcgaccggcg ctatgtgcct ctggtggggc tggacacgcg ctccgaggtt
     4621 gtcgtgccca tcaagagcaa ggaggtcgtc atcggcgtgc tggacatcca gagcgagacg
     4681 ctcaacgcct tcgacgagag cgatgtcgac ctgttgcgcg cgctggccaa ccagctggcc
     4741 gtggccatcg agaacgcgcg cctctatgag caggcgggcc agctggcggc gctggaggag
     4801 cggcagaagc tggcccggga gctgcacgac tctgtctcgc aggcgcttta tggcatcgcc
     4861 ctgggggcgc gcaccgccca caccgtcgtc gaccggacgg cggccatcga cgacgacacg
     4921 cgcggcaagc tgaccgaacc gctggactat gtgctggccc aggccaacgc gggcatggcc
     4981 gagatgcggg cgctcatctt cgagctgcgg ccggagtcgt tgcagacgga agggctggtg
     5041 gccgcgctgc gcaagcagac ggccgccctc agcgctcgcc accagattcc ggtgacgacc
     5101 gagtttggcc cggagcccaa cgtgccgccg gcgcagaagg aaatgctcta tcgcgtggcc
     5161 caggaggcga tgcacaacat cgtcaagcac gcgcgggcga cggccgccga agtgcgtctt
     5221 gtcagcgaaa acggccgcgt ggtgatggaa gtctgcgacg acggccaggg cttcgacatg
     5281 agccaggagt tccccggcca tctgggcctg aagtccatgc gcgagcgcgt tgaacgcggc
     5341 ggcggcctgc tgaccatcgc cagcgccccg gccggcggca cgcgggtgat ggtgaaggtc
     5401 agcacggagt gagcgggagc ggggcagcag gggagagcgc tcttccgtag cggccggaac
     5461 ccgcccctgt cccctgcgcc cctcccctac ctttacccaa gccaatacac gtccgccatc
     5521 cactcaatcg tcacgccggt gaggccaaca atcgacaaca cgagcgcgtt cgttcccggc
     5581 gtcagggcgg cctgcaggtt gagggaagta gcgccggtcg gcatgtagct gacgcggttg
     5641 gcctcgacgg tagcgcctat ggggccgttg tagcagtggg cgacggccag cagggaatag
     5701 acgctggggg ggtagaggtt tgtcgagtgg ccgtaggcgt ggagcaggag ggcgcggccg
     5761 gtgttgggcg ggacgaagag agcaggcgtc agcgtgagca ggccgccgcc caggtcgctg
     5821 gtggtggtcg tggctttggc gtccacgccg ccgatgaggc tggccacggg atcggacgcg
     5881 tcggcgcgct ccaggcgata gcgccaccag gcggcaacct gcgtcagcgt gatgacatcc
     5941 tcggcgctca ggcgtccggc cgccgtggcg acgctctcct cctcgcccag agtgctgccg
     6001 ggtaagacga cgccgcgcct catcgcccac ctccggcggc tccccaggac acgacggcgc
     6061 tttcgcccgt ccgctgcgtc aggatgcggt tgagggcgtt gaggtaggcc agttccagcc
     6121 gccgcgcgtt ggccgccagt tgggccatga gcagactgcc ggcgctgggc gcggcctgtt
     6181 cggcagcctg caggcggcgc gcagcggcga agagaacgta ttgcagcagg acgtggtggt
     6241 gggcgtcggg cacggtcagg tagtcgttga ccgaggccag ggcggcatgg ggccgcgtgg
     6301 cgcgcagccg cagcgtcgct ccggtgggcg gatcgaagct gagcagcagc gccggcgggt
     6361 tggtcaggtc gtggcggggc aggaagtcgt aggcgtccag ggcggcggcg aaggcgcgcc
     6421 ctttataggg ccggcgggtc aggtagacgg ccggctcggt tccttccggg tattccacat
     6481 cgaggatggc ccacgtgtcg cccggcaggg cgtagcggcg cacgccggcc agggccgtca
     6541 gcgtcagctc ggccacacgc ggcaaatgct ggctgtactc gcggatggct tcgttgagaa
     6601 aggtcagcag gtcggcgtcg ctgaattcgg ccgcggcggc gtcgtccagc gccacccggc
     6661 aattggccag cacggccgcc gcggccacgc tccccgccgc ttcggcccgg cggccggttc
     6721 cagtgtgtgt actcatgcgg tgttcctcct gtagtagggg ctaggtgtta ggggctagnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ctaatcccta tcccctaccc
     6901 caccgtctcg gcatccgggt tggcgaagtt ccagccggac ttttgccgcc agaggtagac
     6961 ggtgccgggg tcaaggtaaa aggtgacgcg gccgttctgg tcggtccggc cgctggcgac
     7021 gacgaccgcg ccggcgctat cggtggtgac ccagacgtcg gcgtcggcga tggggtcgcc
     7081 ggcgctggct tcggtgaggg tgtaggtgaa ctcggccgcg cccgcgccgg ggctgttgcc
     7141 ggacatggcc gacacccggg cgtcgaggcg ttggccgatg atccagccgg ccgtgttggc
     7201 cgggtaggtg ccgggcaagg ccgcagccca ggggtcagcc gcggcgccgc cggcgttgtt
     7261 gagctggtcg ccggcgctgc cggtggccag gtggctggag agggcttcgt cccacacggc
     7321 gtcggcgatg gtggcggcgc tgggtgggct ggcggcggcc gatacctggg cgtcgaggta
     7381 gaggccgaag gtgccggcgc tggtgtggcc ggcgcgggct tcgtcccaaa cggcgtcggc
     7441 caactcggcc gcggcgctgg cgtcgagggc gctgctggtg atggcgttgg cggtcacctg
     7501 ggcggcgtcg acgtcgagcc tgtcactgcc aaggacgagg ctgtcgtagg tattggcggg
     7561 cagaacgacg aagtcttgcc agaccgggag cgcgcccgtc ttggagacgg cgacgcgcag
     7621 gcggccgagg gtgctggtgt cggtggtgtt cagggggcag gcgtagaagc cgttctcctg
     7681 gtggctgggg ctggtggcgt cgttcttctg ggcgtaagcg ccgccgtttt tggacaggcg
     7741 gacgtcggcc tgggcgatgg cgaggccggt ttcggccgtt ttgccgtcgg cgtcatcgac
     7801 gaaggggccg agggtgaggg tggcgctggt ggattgtttg aggaacatgg cttagagacc
     7861 tcgtctcagg cggaactggt gctggctgcg ggccggttgg ccagcggcgg ccggttcggt
     7921 gtagtcgatg gtcagttggg gcgcgttgct gccgttgtcg taggcgtaga cgcgcaaggc
     7981 ggagccggcg ttggggaaca ggtagaagtt gatggcgttg ccgctggccc agccggggat
     8041 ggccagaatc tccgcgagga tggcgctcag gtcggggctg ttcctggccc ccgcgccgat
     8101 gctgccggcg ttccaggtga cggtggcggc cgtcttgaag cgggtggtga tgtcgttggt
     8161 cgtgctggtg aaggtggtgg tggtggcttc gccgctgccg cggatggtca ggttggggtc
     8221 gtcgtagctg gtcgagacga cgttgagcgt caggtaggcc gcggtgatgg tcgcgccggc
     8281 cgggatggtg acgttggcga agcgcaggcc gatgttctgg ccggcgctgt tggcgttgag
     8341 gttgacgccg ttgagggtca tcacgccgct gagttcgacg gcatcatcgg ccgaagcggc
     8401 gacggcgcgg gtgagggtgg tcatggtttg gctcctgttg gccgggccgc gcgggccgcg
     8461 ggcgcggccg gtcgtcgcgc ccgtggcccc gcggccgcgg ctcttagctc acgttgcgct
     8521 tgccgatgcc gcggtggccg ttcaccccca gggcaaactc gtcgcgcacc ttgatgggca
     8581 ggatgtcgtt gctaaacatc agcccactcg tctcgctgac gacggcaaac aactccggga
     8641 tgggatgacg ccggccgccg ggagcctggc tgtagctcat ctggatgacc gggaagacgg
     8701 ccggatcgac cacgtaggcc cagttgttgt catcggtcca gtcgggcacg accagcggga
     8761 tgggccgcgg gtcggcaaag ccgcggccga cggcctccgg cgtgtagacc gtgggcatgc
     8821 cctcgccata gccgaggatg gtcagcgccg tgtcatatag ctcggccggc accagcagga
     8881 agcgcgggaa gattcccaac ggcttgccgc tgccaacctc gctgtgctgg aagcattcgg
     8941 cgcgggcggc gcgccaggcg gccgcg
//
LOCUS       DAOSMQ010000066         8878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7843_length_8878_cov_4.910688, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000066 DAOSMQ010000000
VERSION     DAOSMQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7843_length_8878_cov_4.910688"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PK829_03555"
     CDS             <1..1198
                     /locus_tag="PK829_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOU40325.1"
                     /translation="IQIVRNWDEWRQEPLTRAKQQQAEQAAFFIGVPPAVLVHELMHA
                     VAIWLFGGQVIEFGYRVFWGYVVPQGSFSPTQNWIIAIAGTLGSLAFGATLWWLARRS
                     ASSTVRYFGLRAFRFQIYFSLLYYPIMTLFLPIGDWRTIYAFGNTPWLSLATAVAHAG
                     LLLWFWRADRAGAFEMPAFETATARAHHDLSAASGDPLAQLHSIEVLRRGGAPQQALR
                     SLDALLAQYPRFAEAHLQRALIIAQTRGGISREAAGAARQALALGLREPTDVALAYRL
                     LALQPLQQGDGATAQAAMDAALTPAPGYDPDRLPDAQRAELHALRAQAYRRQGRYDDA
                     YREAGVALALAQTAGRDDIIARLRQEQELIEKHAGRKLSAEPLIERSPDYPADDTPGQ
                     LPRSET"
     gene            1397..2860
                     /locus_tag="PK829_03560"
     CDS             1397..2860
                     /locus_tag="PK829_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU40326.1"
                     /translation="MRVELRDIRKVFGAVKANDGISLTLESGKIYALVGENGAGKSTL
                     MKILSGYQPPTSGEILLDDQPVRFQSPADALAQGVGMLYQDPLDMPPFRVIDNYLLGR
                     DSRLVLDYKTARDELAQLAARYGFDIDPNATIDSLSMGQRQQLELVRLLAGGAQVLIL
                     DEPTTGISAEQKEILFESMRRMTNEEGKTVILVSHKLEEVQELCIHAYVLRRGKMVGG
                     QSLPCGNELLVEMMFGHMPQRSRRPLFQLGQPVLELRDVTISTYELTVPDINLSVRAG
                     EVFGFAGLEGSGQRLVMQVCAGLMAPSKGQVYIGGQDVTGWNYHRMQAHGMGYVAAGR
                     LEEGLVAGLSLTEHLVLARPEKQFFIDWKANETEMSQRISTYQIVGRPETTADALSGG
                     NQQRLLFSLLNTPLKLLLLEHPTRGLDVRSADYTWQLLYQRREHGTAILFISADLDEI
                     IAHSDRIAVFSGGRMSRVVEAAQTTAEELGHLIGGQA"
     gene            2857..3993
                     /locus_tag="PK829_03565"
     CDS             2857..3993
                     /locus_tag="PK829_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40327.1"
                     /translation="MNQARTSGASWIARVWPLVPVVAALLFTSGLLLLFHASPGEAYR
                     AMWDGAFGTPDKILSVVAFWIPMVLCSVGLLVTFTAGLWNIGVEGQIVLGAICASWVA
                     LRLSGPAWLLIPLEIGAAMLGGALWAAIAAVLKTRGKVNEIFGGLALNNLAIILTNYL
                     ISGPWQPPEGGTFRGTAPFQDAALLPRFLESRFSPLSLVLAGLAFAAVFFILRGTTWG
                     LKLKALGKNPHSAFLLGVSSERETVLAMMFCGALAGLGGAVRVLSWFDSLRQSISGGI
                     GFLSLLIVMLAAYRILLVPFIAFFFSVVLNGSITLQLKTQLHSALGGILTGVMVLFVL
                     LFGSAKQLGIGKTRAGSGDAPEAIPAAPADSIAGDISTHARGGD"
     gene            3993..4967
                     /locus_tag="PK829_03570"
     CDS             3993..4967
                     /locus_tag="PK829_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40328.1"
                     /translation="MNDSQLIAVLASIVLQASPLIIAVCGEILTERSGIVNLSLDGTM
                     LMSAMTGFVVGMQTDNYWLGFLAAAVVGAIFAAIVAFGSIRLGQSQVAIGFVLTLLGD
                     DLSAFLGQNYVRMTGAVVPRAGIPLLKDLPILGPILFNQNIVVYFAILLIVATWWWLN
                     RTQAGLRLRGAGERPTAAFARGVDVNRVRYLYTILGGALVGIAGAAYSLNVKIGWSDG
                     HVRGFGWIALAIVIFGGWSPIRGAFGALLFGATKALATVLQRAFPEVSVVAFNAIPWA
                     LMILVLLAVGSDYTERFIEVMPRRWQRPLRRILRVSPPMALGTAFHEG"
     gene            4990..6270
                     /locus_tag="PK829_03575"
     CDS             4990..6270
                     /locus_tag="PK829_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40329.1"
                     /translation="MIDFLVRNPRLGRLFDLLLPLLATLAALAVGAIMLQLLGANPFV
                     AYRALLEGAFGSQNALADTIVRATPLLLVGLGICIAFRAGVINIGGEGQFIAGALPAT
                     LLGLSFPDAPAIIIVPAALLCGFLGGAVWGSIPGLLKAYFNVNEILSTIMLNIIALQF
                     MNFLLRGPMIDPVQLQAASAIPQTARLNPAFDLPRWVPTRLHAGALLAVALAVVVYIL
                     LWRTTIGYRIRAVGFNPDASRYAGINVKRNIVLALLLSGAFAGLAGAVQVYGVSHRMI
                     TDGSTAGFTGNAGFNGIVAALFGKLHPLGAIPASFIFGALIVGANSLQRAMQVPAALI
                     TALNGLVVVFVVCSDIFSRRLARRRQIGVVEVGSKGAGEQGGGGAGERPAAAANVGPP
                     DSGGVKPAPPATAAKVSAPIKPVAPVAGKEASSD"
     gene            6263..7204
                     /locus_tag="PK829_03580"
     CDS             6263..7204
                     /locus_tag="PK829_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40330.1"
                     /translation="MISQLFTEAVLIGILTSGIRLATPYLFASIGEMFGQRSGVLNLG
                     VEGMMLMGAFAAFYVTLTTGSPWLGLLASILVGTLMGLAMAVISVTFQAEQGISGIGV
                     YLFGLGMSELLFKKMLGTVETVSGFRPLAIPVLSKLPIVGPILFQQSAMVYLAYLLVP
                     VAWFVLYRTTFGLKIRAVGENPAAADSLGVSVKRTRYMTVTLGGALAGVAGASLSIAL
                     LNVFQQNLTSGIGFIAVALVYFGGWRPYGVLFGALLFSMVNALQLWIQALNIPIPFDA
                     SELLIMMPYVLTILVLMFSAGRVRKPSALGAIYERGT"
     assembly_gap    7501..7600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7601..>8878
                     /locus_tag="PK829_03585"
     CDS             <7601..>8878
                     /locus_tag="PK829_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU40331.1"
                     /translation="EEAAGPFRVAVVMPSGINDLAFSQSMFDALTAIQNEMGADNFEF
                     VYSENMFVVEDAAAALRDYASQGYDLVIGHGSQYGSSLQEIAPDFPDTSFAWGTTVDT
                     FGIPNIYAYEARSEEGGYVNGVLAANLSTSNTLGVVGPIETGDAKLYVDGFVAGAKAA
                     KPDITVNVNYIGSFSDIALATEAANTLVGAGADALTGTAQMVVGAIGVAEENGVPWFG
                     TQSSQTKLAPSVVVANQVYDWTGVLKTIIEQVKAGTLGGESFALTLENGGLVMDYNPD
                     YALPEAAKTAADEAAAGIAAGTISVFGGETTAPAEEPAAEAAPLTFGMVLVGPKNDHG
                     WSQAQAEGGQFIVDNMPGSEMILFESLNPADKPEATLEGVVDDMVANGATLIFTTSDE
                     FEEDTLAVAQKYPDIIFINATGDDAKTGEAPAN"
BASE COUNT         1408 a         2898 c         2836 g         1636 t
ORIGIN      
        1 catccagatc gtccgcaact gggacgagtg gcggcaagag ccactgacca gggccaagca
       61 gcaacaggcc gagcaggccg cctttttcat cggcgtgccg cccgcggtcc tggtgcatga
      121 gttgatgcat gccgttgcca tctggctgtt cggcggccag gtcatagagt tcggctaccg
      181 cgttttctgg ggctacgtcg tgccccaggg cagcttcagc cccacccaga actggatcat
      241 cgctatcgcc ggcacgctgg gatcgctggc ctttggggcg acgctgtggt ggctggcccg
      301 ccgcagcgcc agcagcaccg tacgctactt cgggttgcgc gcttttcgct tccaaatcta
      361 cttctcgctg ctctactacc ccatcatgac gctgttcctg cccattggcg actggcggac
      421 gatctacgcc tttggcaata cgccctggct gagcctggcc acagccgtcg cccacgccgg
      481 gctgctgctg tggttctggc gggccgaccg ggccggcgcg ttcgagatgc ccgcgttcga
      541 gacggccacc gcgcgggcgc atcacgacct gtccgcggcg agcggcgacc cgctggcgca
      601 attgcacagc atagaggtct tgcgtcgcgg cggcgcgcca caacaggccc tccggtcgct
      661 agacgccctg ctggcccagt acccgcgctt tgccgaggca cacttgcagc gggcgctcat
      721 catcgcccag acgcgcggcg ggatcagccg cgaggccgcc ggcgcggccc ggcaggcgtt
      781 ggcgctgggg ctgcgcgagc cgacggacgt cgccctggcc taccggctgc tggctctcca
      841 gccgttgcag cagggcgatg gcgcgacggc ccaggcggca atggacgccg ccctgacgcc
      901 cgcgcccggc tacgaccccg accggctgcc cgatgcgcag cgcgccgaac tccacgccct
      961 gcgcgcccag gcctatcgcc ggcaggggcg ctacgacgac gcttaccgcg aggccggcgt
     1021 cgccctggcc ctggctcaga ccgccggccg cgatgatatc atcgcccgtc tgcgccagga
     1081 gcaggaactc attgagaaac acgccggtcg caagctgtcg gccgagcctc tgatcgagcg
     1141 cagccccgat tatccggccg acgacacacc cgggcagctg ccccggagcg agacgtaacg
     1201 agcctggctg ttgcctttaa cactcatcgc ggcatgcgcc cttcgcatgt catagatgaa
     1261 tgtcatatag aaatcaggat caatggcagt aggcagccat ccggttattc gctattctgt
     1321 ttgagcgggt tggcgctagg agccacacgc gtgtgactgg cctgcggcat cacatggcag
     1381 actaccggga ggtcatatgc gggttgaact gcgggacatc cgaaaggttt ttggcgcggt
     1441 caaggccaat gacggcatca gcctgaccct ggagtcaggg aagatttacg cgcttgtcgg
     1501 cgagaatggc gccggcaaga gcaccctgat gaaaattctc tccggctatc aaccgcctac
     1561 cagcggcgag atcctcctgg acgatcagcc ggtacgcttc caatcgccgg ccgacgcgct
     1621 ggcccagggc gtgggcatgc tctatcagga tccgctggac atgccgcctt tccgcgtcat
     1681 cgacaactac ctcctcggcc gcgacagtcg cctcgtgctc gactataaga ccgcccgcga
     1741 cgaactggcc caactggccg cgcgctacgg tttcgacatc gaccccaacg ccaccatcga
     1801 ctcgctctcc atgggccagc ggcagcagtt ggaactggtg cgcttgctgg ccggcggcgc
     1861 gcaagtgctt atcctcgacg agccgaccac gggcatcagc gcggagcaga aagagattct
     1921 ctttgagtcg atgcgccgga tgaccaacga ggagggcaag accgtcattc tcgtctccca
     1981 caagctggaa gaggtgcagg aactatgcat ccacgcctac gtcttgcggc gcggcaagat
     2041 ggttggcggc cagtcgttgc cctgcggcaa tgaattgctg gtggagatga tgtttggtca
     2101 catgccgcag cgcagccgcc ggccgttgtt ccagttgggc cagccggtgc tggaactgcg
     2161 tgacgtgacc atcagcactt acgaactgac cgtgcccgac atcaatctgt ctgtccgcgc
     2221 cggcgaagtg ttcggcttcg ccgggttgga gggcagcggc cagcgactgg tgatgcaggt
     2281 ctgtgccggg ctgatggccc cgtcgaaggg gcaggtctac atcggcgggc aggacgtgac
     2341 cggctggaac tatcaccgca tgcaagccca tggcatgggc tacgtggccg cggggcggct
     2401 ggaggaaggc ctggtggctg gcctgtcgct gaccgagcac ctggtcttgg cccggccgga
     2461 aaagcagttt ttcatcgact ggaaggccaa cgagaccgag atgagccagc gcatcagcac
     2521 ctatcagatc gttggccggc cggagacgac cgccgacgct ctctccggcg gcaaccagca
     2581 acgactgctc ttctcgctgc tgaacacgcc gctgaagttg ctgctgctgg agcacccgac
     2641 gcgcgggctg gacgtgcgct cggccgacta cacgtggcaa cttctctatc aacgccgcga
     2701 acacggcaca gctatcctgt tcatctcggc cgatctcgac gagatcatcg cccacagcga
     2761 ccgcatcgcc gtcttttctg gcgggcgcat gtcgcgggtc gttgaggcgg cgcagacgac
     2821 ggccgaggaa ctgggtcacc ttatcggagg ccaggcatga atcaggcgcg aacgagcggg
     2881 gccagctgga tcgcccgcgt gtggccgttg gttcccgtgg tcgcggcgct gctgtttacc
     2941 agcggtctgc tgctgctctt ccacgccagc cccggcgaag cctaccgggc catgtgggat
     3001 ggggccttcg gcacgccgga caagattctc agcgtggtcg ccttctggat tccgatggtg
     3061 ctgtgctccg tcggtctgct ggtcaccttc accgccggct tgtggaacat cggcgtggag
     3121 gggcagatcg ttctgggagc catttgcgcc agttgggtgg ccctacgcct cagcgggcca
     3181 gcctggctgc tcatccctct ggagattggc gcggccatgc tgggcggggc actgtgggcg
     3241 gccattgccg cggtgctgaa gacgcgcggc aaggtcaacg aaatcttcgg cggcctggcg
     3301 ctcaacaacc tggccatcat tttgaccaac tatctcatct ctgggccgtg gcagccgccg
     3361 gagggcggca cgttccgcgg cacagccccc ttccaggacg cggccctgct gccgcgcttc
     3421 ctggagtcgc gcttcagccc gctgtcgctg gtgttggccg ggctggcctt tgccgccgtg
     3481 ttcttcatct tgcggggcac gacctggggg ttgaagctaa aggcgctggg caagaacccg
     3541 cactcggcct ttctgctggg cgtctccagc gagcgcgaga cagtcctggc gatgatgttc
     3601 tgcggcgcgc tggcggggct gggtggggcc gtgcgcgtcc tcagctggtt cgacagtctg
     3661 cgacagagca tctccggcgg catcggtttt ctgtcgctcc tcattgtcat gctggccgcc
     3721 tatcgcatcc tgttagtgcc cttcattgcc tttttcttct cggtcgtgct caacggcagc
     3781 atcactttgc agctaaagac ccaactgcac tcggctctgg gcggcatctt gaccggcgtg
     3841 atggtgctgt tcgtgctgct ctttggctcg gccaagcagt tgggcattgg caagaccagg
     3901 gccggctctg gcgacgcgcc cgaagcgatt ccggccgccc cggccgacag catcgccggc
     3961 gacatctcga cccacgcgcg gggaggcgac tgatgaacga cagccaactc attgccgtcc
     4021 tggcctccat tgtcctgcaa gcgtcgccgc tcattatcgc cgtttgtggc gaaatcctga
     4081 ccgagcgctc tggaatcgtc aacctgtccc tcgacggcac gatgctcatg tcggccatga
     4141 ccggctttgt cgtcggcatg caaaccgaca actactggct cggcttcctg gccgcggccg
     4201 tggttggagc catcttcgcg gccattgtgg ccttcggcag catccgtctg ggccagagcc
     4261 aggtggccat cggcttcgtg ctgacgctgt tgggcgacga cctgagcgcc ttcctgggcc
     4321 agaattacgt ccgcatgacc ggcgccgtcg tgccgcgggc gggcattccg ctgctaaaag
     4381 acttgcccat tctcggcccg atcctgttca accagaacat cgtcgtctac ttcgccatct
     4441 tgctgatcgt agcgacgtgg tggtggttga accggacgca agcggggcta cggttgcggg
     4501 gcgcgggcga acggccgacc gctgcctttg cccgtggcgt cgatgtcaac cgggtgcgtt
     4561 acctctatac catcctcggc ggcgcgctgg tcggcatcgc cggcgcagcc tactcgctca
     4621 acgtcaagat cggctggtct gacggccacg tgcgcggctt cggctggatc gccctggcca
     4681 tcgtcatctt cggcggctgg tcgcccattc gcggcgcgtt cggcgcgctg ctctttgggg
     4741 caacaaaggc gctggccaca gtcttgcagc gcgcctttcc ggaggtttcg gtagtcgcct
     4801 tcaatgccat tccctgggca ttgatgatcc tggttttgct ggccgtcggc agcgactaca
     4861 cggagcggtt tatcgaggtc atgccgcggc ggtggcagcg tccgctgcgc cgcatcctac
     4921 gtgtctcgcc gccaatggct ctgggaacag cttttcatga gggatagtgc cctccgcgcg
     4981 gagtagccca tgatcgactt cctcgtcaga aatccgcggc ttggtcggtt gttcgatctt
     5041 ctcttgcctt tgctggccac gctggccgcc ctggccgttg gggcgatcat gctccagctg
     5101 ctgggcgcga acccatttgt cgcctaccgc gccctgctgg aaggcgcgtt cggcagccag
     5161 aacgccctgg ccgacaccat cgtgcgggcc acgccgctgc tgctggtcgg cctgggcatc
     5221 tgtattgcct ttcgcgccgg ggtgatcaac atcggcggcg aggggcagtt cattgccggg
     5281 gcgctgccgg cgacgctgct ggggttatcc tttcccgacg cgccggccat catcatcgtg
     5341 ccggcggcgc tgctgtgcgg tttcctgggc ggggcggtct ggggcagcat ccccggtctg
     5401 ctgaaggcct acttcaacgt caacgaaatc ctcagcacga tcatgctcaa catcatcgcc
     5461 ctgcagttca tgaacttcct gctgcgcggc ccgatgatcg acccggtgca actgcaagcg
     5521 gcttcggcca ttccccagac ggcgcggctg aacccggctt tcgacctgcc gcgctgggtg
     5581 ccgacgcggc tgcatgccgg ggcgctgctg gcggtcgcgc tggccgtggt ggtctacatt
     5641 ctgctgtggc gcacgaccat cggctatcgc atccgcgccg tcggcttcaa ccccgacgcc
     5701 tcgcgctacg ccggcatcaa cgtcaagcgc aacatcgtcc tggcgctgct gctcagcggc
     5761 gcgtttgccg ggctggccgg ggcggtgcag gtctacggcg tcagccaccg catgatcacc
     5821 gacggctcaa cagccggttt caccggcaac gccggcttca atggcatcgt cgccgccttg
     5881 ttcggcaagc tgcacccgct gggggccatc ccggcctcgt tcatcttcgg cgcgctcatc
     5941 gtcggagcca acagcttgca acgggcgatg caggttccgg cggcgctcat caccgcgctg
     6001 aacggcctgg tggtcgtctt cgtcgtctgc agcgacatct ttagccggcg gctggcgcgg
     6061 cggcggcaga tcggtgtcgt cgaggtgggt agtaaaggcg ccggagagca ggggggcggg
     6121 ggagccgggg aaagaccggc ggcggccgcg aacgtcggcc cgccggatag cgggggcgta
     6181 aaacccgcac cgcccgcgac tgcggccaag gtcagcgcgc cgatcaagcc ggtcgccccc
     6241 gtcgcgggca aggaggcgtc cagtgattag ccagctattt accgaagcgg ttctgatcgg
     6301 catcctgacc tccggcatcc gcctggccac gccctatctg ttcgcctcca tcggcgagat
     6361 gttcggccag cgcagcggcg tactgaacct gggcgtcgaa ggcatgatgc tcatgggcgc
     6421 gtttgccgcc ttctacgtca ctctgaccac cggtagcccc tggctgggat tgctggcgtc
     6481 catcctcgtc ggcacgctga tgggcctggc catggccgtc atcagcgtca cctttcaggc
     6541 cgagcagggc atcagcggca tcggcgtcta cctgtttggg ctgggcatga gcgagctgct
     6601 attcaagaag atgttgggca cagtggagac agtcagcggc ttccggccgc tggccatccc
     6661 agtgctcagc aaactcccca tcgtcggccc gatcctgttc cagcagagcg ccatggtcta
     6721 cctggcctat ctgcttgtgc cggtggcctg gttcgtgctc taccggacga cgttcgggct
     6781 gaagatccgc gccgtgggcg agaacccggc cgcggccgac tcgctgggcg tcagcgtcaa
     6841 acggacgcgc tacatgaccg tcaccctggg cggcgcgctg gccggtgtgg ccggggcgtc
     6901 gctgtccatt gccctgctga acgtcttcca gcagaacctg accagtggca tcggcttcat
     6961 cgccgtggct ctggtctact tcggcggctg gcggccttat ggcgtcctgt ttggcgcgct
     7021 gctgttcagc atggtcaatg ccttgcagct gtggattcag gcgctcaaca ttcccattcc
     7081 gttcgacgcc tctgagctgc tgatcatgat gccctacgta ctgaccatcc tggtcctgat
     7141 gttctcggcc gggcgcgtgc gcaagccgtc ggcgctcggc gctatctatg aacgcggcac
     7201 ataatccacc tgaaggaggt gatgcgctcc gatatttggc tgtcaactgg ttgggcgggg
     7261 tcgcaccgcg gcctacgctc ctgattcgtc gtcaccatca atgaaaaaca tttccatgcg
     7321 aatccaaaag cacaatgcaa aggagaggaa gaacatgaaa cgcactttgt ggctgagctt
     7381 gctgctaatc ctggccatgg ttctcgtggc ctgcggcggc gctcagaccg acacgactgt
     7441 cgaagagcag cccgcggcca cggaagagac cacggccgag gaagctgcgc cgaccgaaga
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgaagaggc cgccggcccc
     7621 ttccgcgtcg ccgtcgtcat gcccagcggt atcaatgacc tggccttcag ccagagcatg
     7681 ttcgatgccc tgacggctat ccagaacgag atgggcgcgg acaacttcga gtttgtctac
     7741 tcggagaata tgttcgtggt agaagacgcg gctgccgccc tgcgcgacta cgccagccag
     7801 ggctatgacc tcgtcatcgg ccacggctcg cagtatggca gctcgctaca ggagatcgcc
     7861 cccgatttcc cggacacgag ctttgcctgg ggcacaacgg tcgatacctt cggcatcccc
     7921 aacatctatg cttatgaggc gcgctccgaa gagggcggct acgtcaacgg cgtgctggcg
     7981 gccaacctgt ccaccagcaa caccctgggc gtggtcggcc ccatcgagac gggcgacgcc
     8041 aagctctacg tcgacggctt cgtggccggg gccaaggcgg ccaagcccga catcaccgtc
     8101 aacgtcaact acatcggctc cttcagcgac atcgccctgg cgaccgaagc ggccaacacg
     8161 ctggtcggcg cgggcgcgga tgccctgacg ggcacggcgc agatggtcgt cggcgcgatc
     8221 ggcgtggccg aagagaacgg tgtgccctgg tttggcacgc agtcgagtca gacaaagctg
     8281 gcccccagcg tcgtcgtcgc caaccaggtc tatgactgga caggcgtcct gaaaaccatc
     8341 atcgagcagg tcaaggccgg cacgttgggt ggcgagtcct tcgccctgac gctggagaac
     8401 ggcggcctgg tgatggacta caaccccgat tacgccctgc ccgaagcggc caagacggcc
     8461 gccgacgaag ccgcggccgg catcgccgcg ggcacgatca gcgtcttcgg cggcgagacc
     8521 acggccccgg ccgaggaacc ggccgccgaa gccgcgccgc tgacgtttgg catggttctc
     8581 gtcgggccga agaacgacca cggctggagc caggcgcagg ccgagggcgg ccagtttatt
     8641 gtggacaaca tgcccggctc ggaaatgatc ctgttcgagt cgctgaatcc ggccgacaag
     8701 ccggaggcga cgctggaagg cgtggtcgat gacatggtcg ccaacggcgc gacactcatc
     8761 ttcaccacct ccgacgagtt tgaggaagat actctcgcgg tggcccagaa gtaccccgac
     8821 attatcttca tcaacgccac tggcgacgat gccaagacgg gcgaggcccc ggcgaatc
//
LOCUS       DAOSMQ010000067         8771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7980_length_8771_cov_4.205828, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000067 DAOSMQ010000000
VERSION     DAOSMQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7980_length_8771_cov_4.205828"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /gene="sucD"
                     /locus_tag="PK829_03590"
     CDS             <1..812
                     /gene="sucD"
                     /locus_tag="PK829_03590"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOU40332.1"
                     /translation="GTFHTNQMIAFGTHVVGGVVPGKGGQSFEGEGATVPIFDSVREA
                     AEATGANASIIFVPARFAPDAMIEAADAGLELVVCLTEHIPVLEMINVRAYLDQRNTR
                     LIGPNCPGLITPGGAKVGIIPSSIVSPGHVGLVSRSGTLTYEVVYALTQRGIGQSTCV
                     GIGGDPIKGMDFIDVLRLFEEDPETSEVIMIGEIGGNAEEQAAAFVANHMSKRVTAFI
                     AGRAAPPGRRMGHAGAIVEGKSGTAEGKITALQAAGVQVADNPDRIVEMIS"
     gene            893..>1129
                     /locus_tag="PK829_03595"
     CDS             893..>1129
                     /locus_tag="PK829_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40333.1"
                     /translation="MKQLWRRFDAGFLVVLLISLLAVWPFLSRASLPEGTDAELHIFR
                     LHELGYLIHGGEFYPRWAPNFYHAYGYPIFNYYAP"
     assembly_gap    1130..1229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1230..3369
                     /locus_tag="PK829_03600"
     CDS             <1230..3369
                     /locus_tag="PK829_03600"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HOU40334.1"
                     /translation="APNFYHAYGYPIFNYYAPLTYYLALPLELLPQFDATAASKAVLV
                     AGMLLAGLGIYGFARDNWGRRAGYVAAALYVYAPYLQYVDPHIRGALPEVFSFAVFPL
                     ALWALDRLRRRPTPGAWVAAAGLVAAVILSHNLMGLFFYGLLFAWVLWQMVVIERRPA
                     ARLLAVLLVGLGLAAFFWLPVILERDAVNLTTLIGANDNYDFHTHFVGLVELLGGSLQ
                     PDWGATQPAFRFNLGVAQWLAGLLGVVMLALGRVRHRPQVLFFALAAAAVILLMTPLA
                     TPIWEALPFLPYFQFPWRLLGGAAALLAVLGAAGVEAVARAVTADANGHGDESAVGPR
                     RSSALVYSIAVALPMLFALSLSQPTPWAPFGEVNTARMSQLENSGRWLGTTSTADYVP
                     ATVEMLPPRRPQMVDPLAVGEQPDRVNREAMPDGAVVVAEYVRPLTTRYHVTAPKQFR
                     LRLYQFDFPGWQVTIDGEPATTELAAPEGLIVVLVPQGEHVVTVHFGSTAARTLAWGI
                     TTAALLLAAAGAWRLRRRSAAPPAVEGFAARADWPIVVVAAAITLAVIALEPLGLFHV
                     HSQNGALAIPATAHPANFGDQIALLGYHAGGPTAVPGETVELTLFWQAQRPLDIEYQV
                     FVHVLGPDGQPVAQSDKINPGDFPTHRWPMGKYVPDTHRLTLPADLPPGSYPVVTGLW
                     VQSEGWRLPVFDAGGAVVGDAETLFTLEVR"
     gene            3371..3937
                     /gene="rsmD"
                     /locus_tag="PK829_03605"
     CDS             3371..3937
                     /gene="rsmD"
                     /locus_tag="PK829_03605"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOU40335.1"
                     /translation="MSARVIGGKARGRRLKLVPGDTTRPIMDRVKESLFNILGDVGGT
                     RWLDLFAGTGQVGIEALSRGAAGVVFTDNARAAVQTIRDNLAYTRLTDGATVQQIDAF
                     RFLRETSALPFDIIFVAPPQYKELWSAALTAIDAAADRMLAAGGQVVVQIDPREFRET
                     PLQQLELSDKRRYGNTLLCFYERPAALA"
     gene            complement(3962..4729)
                     /locus_tag="PK829_03610"
     CDS             complement(3962..4729)
                     /locus_tag="PK829_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40336.1"
                     /translation="MDRVYLFLIHNDVWIYILSTLGLFWYLTELVRAQTALRRAMFNL
                     ERETATRLRNHALSFVLFLSAVIGIVYYVNNYIAPDLPQELTAEPTATPDIFATPLPS
                     PTPLGTPLAEGVPDNTIILAPTATLPGAAVSAPITDTETTAAAAEPTPFSACTPELRL
                     DEPLDGSVAFGRVRFRGTANTGELHQYIIELNGQQTEGLWAPITQEPQNQPIVNGDLG
                     EADLSLWQPGPYQVQLRALDIDGAQVGVCTVSFTLDG"
     gene            complement(4785..5645)
                     /locus_tag="PK829_03615"
     CDS             complement(4785..5645)
                     /locus_tag="PK829_03615"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU40337.1"
                     /translation="MSEFISHEQVVNAANFVRANITHNHRPAVGLILGSGLGALADAI
                     ESPETVATADVPHWPASTVQGHSGRLVIGSMGGQTALVLQGRTHFYEGYSMGHVTLPI
                     RVMIELGIRTLIVTNAAGGLNPDYEPGDLMLISDHINFPGMAGNNPLRGPNDDSYGPR
                     FPDMTAPYDAALRRLAHQVAAGAGFALREGVYAYVGGPSFETPAELRFLQRAGADAVG
                     MSTAPEVVVARHAGVRVLGISTIANLALPDPKVGIIQTHQEVLEMSARAVPRLVAVAS
                     GVVGRLAEVA"
     gene            complement(5642..6526)
                     /locus_tag="PK829_03620"
     CDS             complement(5642..6526)
                     /locus_tag="PK829_03620"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOU40338.1"
                     /translation="MDEHLLAFLSYLEREYQYSDNTVAAYHNDLSQFLDYLEQHKPLS
                     EWSAAAEDDIEAYLAYMKQKEQPYASSTIARKVAAIKSFFNYLTAQGVVAENPTLQVE
                     SPKVKKRLPQTLNLSEVEQLLDAPADDQSPKSLRDATLLNVLYETGMRVTEVVSLQME
                     DVDLEAATLSSPTRQGEARVIPIPETTQTLLARYLAAGRPQLVHNAAEHALFLNHRGE
                     KLTRQGLWLIIKGYARQAGLKADVTPHTLRHSFAVHRLGRGLKLEEIQHLLGHANIST
                     TQIYTQMEAPDEESPNGS"
     gene            6856..8193
                     /locus_tag="PK829_03625"
     CDS             6856..8193
                     /locus_tag="PK829_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOU40339.1"
                     /translation="MSSHSPQRILIAGAAGRDFHDFNTVFRDDDAYRVVAFTATQIPN
                     IEGRLYPPALSGPLYPEGIPIYPEAELEELIARHEIDDVVFSYSDVSHETVMHLASRV
                     LAAGAGFRLMNPRRTQLASTRPIVSVGAVRTGSGKSQTSRRILSVLQNDLGYEHVVAV
                     RHPMPYGNLAEQAVQRFASHADMDRHKCTIEEREEYEPYVERGAVIFAGVDYEAILRK
                     AEQEADIIIWDGGNNDLPFFRSDYHVVVVDPHRPGHEMRYHPGEANVRLADALVVNKI
                     DTAEPSGVDTVLRNIRQLNPRAAIVRGASPLFVDDPAAIQGRRVLVVEDGPTLTHGEM
                     AYGAGVVAARQFGAAEIVDPRPYAVGTIAATYAKYPNTGPVLPAMGYSAEQIADLEET
                     INRTPVDMVIVATPIDLARLVDIDRPSQRVRYELQEIGQPTLTDLLRAHFGRG"
     gene            complement(8353..>8771)
                     /locus_tag="PK829_03630"
     CDS             complement(8353..>8771)
                     /locus_tag="PK829_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU40340.1"
                     /translation="ICDHAVAFGVGQADAPTIDRVNILAATRLAMVAAIHALAPAAAA
                     LLIDGPIRLAAVALPQRGVIRGDRLSLSIAAASILAKVSRDRHMIALDAQYPGYGFAR
                     HKGYGTAEHLAALAALGPCPEHRLSFAPLRPAAGAE"
BASE COUNT         1389 a         2902 c         2843 g         1537 t
ORIGIN      
        1 agggcacatt ccacaccaac cagatgatcg ccttcggcac ccacgtcgtt ggcggcgttg
       61 tgccgggcaa gggcggccag agcttcgagg gcgagggggc caccgtaccc atcttcgact
      121 ctgtgcgcga ggcggccgag gctaccggag ccaatgcgtc gatcatcttc gtcccggcgc
      181 gcttcgctcc cgacgccatg atcgaggcgg ccgacgctgg gctggagctg gtggtctgcc
      241 tgacggagca cattcccgtg ctggagatga tcaacgtccg cgcctacctt gaccagcgca
      301 acacccgcct gatcggcccc aactgccccg gcctgatcac cccgggcggg gccaaagtcg
      361 gcatcatccc cagctccatc gtctcccccg gccacgtcgg cctggtctcg cgcagtggca
      421 cgctgaccta cgaggtcgtc tatgccctga cgcagcgggg catcggccaa tcgacctgcg
      481 tcggcatcgg cggcgacccc atcaagggca tggacttcat cgatgtcttg cgcctgttcg
      541 aggaagaccc ggagacctcg gaagtgatca tgatcggcga gatcggcggc aacgccgagg
      601 agcaggccgc cgcttttgtg gcaaaccaca tgagcaagcg cgtaacggcc ttcattgccg
      661 ggcgggccgc gccgccaggc cgccgcatgg gtcacgccgg ggccatcgtc gagggtaaga
      721 gcggcacggc cgagggcaag atcactgccc tgcaggccgc cggcgtccag gtggccgaca
      781 accccgaccg catcgtcgag atgatcagtt agggattagg ggctagggat tagggattag
      841 ggcagaacgc tcttccctac accctaatcc ctaatccctc atccctcttt gcatgaaaca
      901 actgtggcgc cggttcgacg cgggcttcct cgtcgtcctg ctgatcagcc tgctggccgt
      961 ctggcccttt ctcagccggg ccagcttgcc ggaggggact gacgccgaac tgcacatctt
     1021 ccggctgcac gaactcggct atctgatcca cggcggcgag ttctatcccc gctgggcgcc
     1081 caacttctac cacgcctacg gctaccccat cttcaactac tatgcgccgn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcgcccaact tctaccacgc ctacggctac
     1261 cccatcttca actactatgc gccgctgacg tactacctgg ccctgccgct ggagttgctg
     1321 ccacagttcg acgccacggc ggccagcaag gccgtcctgg tcgccgggat gctgctggcc
     1381 gggctgggca tctatggctt cgcgcgcgac aattgggggc ggcgcgccgg ctatgtcgcc
     1441 gccgccctct atgtctacgc gccttacttg cagtacgttg acccccacat ccgcggcgcg
     1501 ctgccggagg tgttcagctt cgccgtgttt cccctggccc tctgggcgct cgaccggctg
     1561 cgccgccggc cgacgcccgg cgcgtgggtg gccgcggccg gcctcgtcgc cgccgtcatc
     1621 ctgagccaca acctgatggg gttgttcttc tatgggctgc tgttcgcctg ggtattgtgg
     1681 cagatggtcg tcatcgagcg gcggccggcg gcccggctgc tggccgtgct gctggtcggg
     1741 ctggggctgg cggccttctt ctggctgccg gttatcctgg agcgcgacgc cgtcaacctg
     1801 acgacgctca tcggggccaa cgacaactac gattttcata cccacttcgt cggcctggtc
     1861 gaactgctgg gcggctcgct ccagccggat tggggggcga cgcagccggc ctttcgcttc
     1921 aacctgggcg tggcccaatg gctggccggg ctgctgggcg ttgtgatgct ggccctgggc
     1981 cgcgtgcgcc accggccgca ggtgcttttc ttcgccctgg ccgccgccgc tgtcatcctg
     2041 ctcatgactc cgctggccac gcctatctgg gaagcgctcc cctttctgcc ctacttccaa
     2101 tttccgtggc gcttgctggg tggggcggcg gcgctgctgg ccgtcctggg cgcggccggc
     2161 gtcgaggccg tggcccgcgc ggtaactgct gacgcgaacg ggcacggcga cgaatcagcc
     2221 gtcggcccgc ggcggtcgtc ggccctcgtc tattccatcg ccgttgccct gccgatgctc
     2281 ttcgccctgt cgctcagcca gccgacgccc tgggctccct ttggcgaggt caacacggcg
     2341 cgcatgtcgc aactggagaa cagcggccgc tggctgggca cgacctctac ggccgactac
     2401 gtgccggcca cggtcgagat gttgccaccg cgccggccgc agatggtcga tccgctggcc
     2461 gtgggcgagc agcccgaccg cgtcaaccgc gaggccatgc ccgacggggc tgtcgtggtg
     2521 gccgaatacg tccggccgct gaccacgcgc taccacgtca ccgcgcccaa acagttccgc
     2581 ctgcgcctct accagttcga cttccccggc tggcaggtga ccatcgacgg cgagccggcg
     2641 accacggaac tggccgcgcc cgaagggctg atcgtcgtcc tcgtgccaca gggggagcac
     2701 gtcgtcacgg tgcacttcgg cagcacagcg gcgcggacgc tggcctgggg cattaccacg
     2761 gcggcgctgc tgctggccgc ggccggcgcg tggcgactgc gccgccggtc cgccgcgccg
     2821 ccggcggtgg agggtttcgc ggcgcgggcc gactggccca tcgtcgttgt ggccgcggct
     2881 atcaccctgg cggtcatcgc tttggaaccg ctgggcctct tccacgtcca ttcgcaaaac
     2941 ggcgcgctgg ctatcccggc tacggcccac ccggccaact ttggcgacca gatcgccctg
     3001 ctgggctatc acgccggcgg cccgacggcc gtgcccggcg aaacggtcga gctcactctc
     3061 ttctggcagg cgcagcggcc gctggacatc gagtatcagg ttttcgttca cgttcttggc
     3121 cccgacggcc agccggtggc ccaatcggat aagattaatc ccggcgactt cccaacccac
     3181 cgctggccga tgggcaaata tgtgcccgac acccaccggc tgacgctacc ggccgacctg
     3241 ccgccgggca gctatccggt cgttaccggc ctgtgggtgc aaagcgaggg ctggcggctg
     3301 ccagtcttcg acgccggcgg cgcggtcgtg ggcgatgccg aaacactgtt tacgctggag
     3361 gtgcgctgaa atgtccgcgc gggtcattgg cggcaaagcc aggggacggc ggctcaagct
     3421 cgtgccgggc gacaccaccc ggccgatcat ggaccgcgtt aaagaaagcc tgttcaacat
     3481 cctcggcgat gtcggcggca cgcgctggct ggacctcttc gccggaacgg gacaggtggg
     3541 cattgaggca ctcagccggg gcgcggccgg ggtcgtcttc accgataacg cccgcgccgc
     3601 cgtccagacc atccgcgaca atctggccta tacgcggctg accgatggcg ccacagtgca
     3661 gcagatcgac gccttccgct ttctgcggga gacgagcgcg ctgcctttcg atatcatctt
     3721 tgtcgccccg ccgcagtata aggagttgtg gagcgccgcc ctgacggcca ttgacgctgc
     3781 cgccgaccgc atgctggccg ccgggggcca ggtcgttgtg cagatcgacc cacgcgaatt
     3841 cagggagacg ccgctgcaac aactggaact gagcgacaag cggcgctatg gcaacacgct
     3901 gctgtgcttc tacgagcggc cggcggcact ggcgtgagtc gcgtgcccgg ccgcgcccct
     3961 tctagccgtc tagcgtaaac gagaccgtac agacgccgac ctgcgccccg tcgatatcca
     4021 gggcgcgcag ttgcacctga taggggccgg gctgccacag gctgaggtcg gcttcgccta
     4081 gatcgccatt gacaatcggc tggttctgtg gctcctgggt gatgggtgcc cacaggccct
     4141 ccgtctgctg gccgtttagc tcgatgatgt actggtgcag ttcgccagtg ttggctgtgc
     4201 cgcggaaacg tacgcgccca aaggcgaccg aaccgtcgag cggctcatca aggcgcagtt
     4261 ccggcgtgca ggcgctgaac ggcgtcggtt cggccgcggc cgcggtggtt tcggtatcgg
     4321 tgatcggcgc ggacacagcc gcgccgggca gggtggctgt gggggccagg atgatggtgt
     4381 tgtctggcac gccttcggcc agcggcgtgc ccaggggcgt gggcgagggt agcggtgtgg
     4441 cgaagatgtc gggtgtggct gtgggttcgg ccgtcagttc ctggggcaag tccggcgcga
     4501 tgtagttatt gacataatag acaatgccga tcaccgcgct caggaaaagg acgaagctga
     4561 gcgcgtggtt gcgcaggcga gtggccgtct cgcgctccag gttaaacatc gcccgtcgca
     4621 gggccgtttg cgcccgcacc aactcggtca gataccagaa cagaccgagg gtgctgagga
     4681 tgtagatcca gacgtcgttg tggatcagaa agaggtagac gcggtccatg ggctagggcg
     4741 tgacagcgta ggcccagaag agcggccgtg gcgcaatgcg ggcgtcaggc gacctcggcc
     4801 aggcgaccga ccaccccact ggccacggcg acgagccgcg gcacggcccg ggcgctcatc
     4861 tccagcacct cttgatgcgt ctggatgatg cccaccttgg ggtcgggcag ggccaggttg
     4921 gcgatggtgg agatgcccag cacgcgcacg ccggcatggc gggccacgac gacctccggc
     4981 gcggtagaca tgccgacggc atccgcgccg gcacgctgca agaagcgcaa ctcggccggc
     5041 gtctcgaaac tcggcccgcc gacgtaggca tagacgccct cgcgcagggc aaagccggcg
     5101 ccggcggcca cctggtgggc caggcggcgc agggccgcgt cgtaaggcgc agtcatgtcg
     5161 gggaagcgcg gcccatagct atcgtcgttc ggcccgcgca gagggttgtt gccggccatg
     5221 ccggggaagt tgatgtggtc gctgatgagc atcaggtcgc ccggttcgta gtcgggattt
     5281 agcccgccgg cggcgttggt gacgatcagc gtgcgaatgc ccagttcgat catgacacga
     5341 atgggcaacg tcacgtggcc catgctgtag ccttcataaa agtgcgtccg cccctgcaag
     5401 acgagcgccg tctgcccgcc catcgaaccg atgaccaggc ggccgctgtg cccttgcacg
     5461 gtggaggctg gccagtgggg cacgtcggcc gtggccacgg tttcgggcga ttcgatggcg
     5521 tcggccagcg cgcccaggcc agagccgagg atcagaccga cggccggccg gtggttgtgg
     5581 gtgatgttcg cccggacaaa attggcggca ttaacgacct gctcatggga gatgaattcg
     5641 ctcatgatcc gttgggtgac tcctcgtcag gcgcttccat ttgcgtatag atttgcgttg
     5701 ttgagatgtt ggcgtggccc agcagatgct gaatctcctc cagcttcaga ccgcgcccca
     5761 ggcgatggac ggcaaagctg tggcgcaggg tgtggggcgt cacgtccgcc ttcaaccccg
     5821 cctgccgcgc ataccccttg atgatcagcc acaacccctg tcgtgtcaac ttctccccgc
     5881 gatgattaag gaaaagggcg tgctccgcgg cgttgtggac gagttgcggc cgtccggcag
     5941 ccagatagcg agccagtagc gtctgcgtcg tctccggaat ggggatgaca cgggcctcac
     6001 cctgccgcgt tggggaagag agtgtcgccg cctccaaatc gacatcctcc atctggagag
     6061 agacgacctc ggtcacgcgc ataccggtct catagagcac attgagcagg gttgcgtcgc
     6121 gcaggctctt gggcgattgg tcgtcggccg gggcgtcgag cagttgctcg acctcgctca
     6181 ggttcaacgt ctgcggcagc cgctttttga ccttgggcga ttcgacttgc agagttgggt
     6241 tctcagccac aaccccctgc gcggtcagat agttaaagaa ggatttgatg gccgccactt
     6301 tgcgggcgat ggtagaggaa gcatagggct gctccttctg cttcatatag gccaggtagg
     6361 cctcaatgtc gtcttcggcc gcggccgacc actccgacaa cggcttatgc tgctccagat
     6421 agtccaggaa ctggctcagg tcattgtggt aggccgcaac tgtgttgtcg gagtactggt
     6481 attctcgttc caggtaagat aggaaggcaa gcaggtgctc gtccatggct tcttactccc
     6541 agatcggttg ccgaatcgac atgcccactg cattcgtaac acgagggagg ttggccgtgg
     6601 cgggcagcca gagtgataca tctcctctca ggtcagaagt tgccgtcacc gtctgcctgg
     6661 ctgggaagaa gccctcttcc cgcgcagccc caaacagatt atgtcacgca acttactggc
     6721 attatagcac tacttttcat aatatcaaat gcctgttaga ccagtttcag gtagtatcct
     6781 cttgccgggc atgccacagc cggccattcc caatatcccc aaccgggcga aatcacgtta
     6841 ggagcaggta tccctatgag ttcccactct ccacagcgca tcctgatcgc cggcgcggcc
     6901 ggacgcgact ttcacgactt caacaccgtc ttccgcgacg acgacgccta ccgggtcgtg
     6961 gcctttacgg cgacccagat ccctaacatc gaaggccggc tttacccgcc agccctctcc
     7021 ggccccctct atccggaggg tattcccatc tatcctgagg ccgagctaga agaactcatc
     7081 gcccgccacg aaatcgacga tgtcgttttc tcttattccg acgtctctca tgagacggtc
     7141 atgcacctgg cctcgcgcgt gctggccgcc ggggccggct tccggctgat gaacccgcgc
     7201 cggacgcaac tggcctcgac gcggccgatt gtctccgtgg gcgcggtgcg cacgggcagc
     7261 ggcaagagcc agacctcgcg ccgcatcctg tctgtgctgc aaaatgatct gggctacgaa
     7321 catgtcgtcg ccgtgcgcca ccccatgccc tacggcaacc tggccgagca ggcggtgcag
     7381 cgcttcgcca gccacgccga catggatcgc cacaagtgta ctatcgagga gcgcgaggaa
     7441 tacgagccgt acgtcgagcg cggcgcggtc atcttcgccg gcgtcgatta tgaggccatc
     7501 ctgcgcaagg cggagcaaga ggccgacatc atcatctggg acggcggtaa caacgacctg
     7561 cctttcttcc gctccgacta ccatgtcgtc gtggttgacc cccaccggcc gggccacgaa
     7621 atgcgctatc accccggcga ggccaacgtg cgcttggccg acgcgctggt ggtcaacaag
     7681 atcgacacgg ctgagccgtc cggcgtcgat accgtcctgc gcaacatccg ccagttgaac
     7741 ccgcgcgcgg ccatcgtccg cggcgcgtcg cccctgttcg tcgatgatcc ggccgccatc
     7801 cagggccggc gcgtgctcgt cgttgaggac ggcccaacgc tgacccacgg cgagatggcc
     7861 tacggggcgg gcgtcgtcgc cgcgcggcag ttcggcgcgg ccgagatcgt cgatccccgc
     7921 ccctacgccg tgggcaccat cgccgccacc tacgccaagt accccaacac cggccccgtc
     7981 ttgccggcga tgggctacag cgcggaacag atcgctgacc tggaagagac aatcaatcgc
     8041 acgccggtcg atatggtcat tgtcgccacg cccatcgacc tggcccggct ggtggacatc
     8101 gaccggccca gccagcgcgt acgctacgag ttgcaggaga tcggccagcc gacgctgacc
     8161 gacctgctgc gcgcgcactt cggccgcggc taaccacccg gtgcgtggcg cgattctccg
     8221 aatcgcgttc ttctgcccgc agcgtggcgc gattctccga atcgcgttct tctgcccaca
     8281 gcgtggcgcg attctccgaa tcgcgttctt ctgcccatcg gcttaagatc gcgattcggg
     8341 agaatcgcgc cactattctg cgccggctgc cgggcgcagg ggcgcgaagc tgagccggtg
     8401 ttcggggcac ggccccagcg cggccagcgc cgccaggtgc tcggctgtgc catagccctt
     8461 gtggcgggca aagccatagc cgggatactg cgcgtccagg gcgatcatgt ggcggtcgcg
     8521 gctgaccttg gccaggatcg acgcggcagc gatgctcagg ctcagacggt cgccgcggat
     8581 gacaccacgc tggggcaagg ccacggccgc cagccggatc ggcccgtcga tgagcagggc
     8641 cgccgcggcc ggggccaggg cgtggatggc cgcgaccatg gccagccgcg tggccgccag
     8701 gatgttgacg cggtcgatgg tgggagcgtc ggcctgaccg acgccaaagg cgacggcgtg
     8761 gtcgcagata a
//
LOCUS       DAOSMQ010000068         8758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_7998_length_8758_cov_4.944617, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000068 DAOSMQ010000000
VERSION     DAOSMQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7998_length_8758_cov_4.944617"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="PK829_03635"
     CDS             complement(<1..1400)
                     /locus_tag="PK829_03635"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOU40341.1"
                     /translation="MTNFVSPSGAGRRALALLVLVLLVALTTVSVGAQGTPDAPTDIP
                     DTELQLEPVILSETRDGDATTVTIEIPVGADTFTASGRPATNFSTDSFIRVGFNSAIN
                     GAQRGFLFFNLSSIPRNAVVQNATLRAFVAGFAPTNDAPFGILARFLSTAWDPTILTW
                     NNFNPQWGAEIGVGQIPAATGWVEANVTAPVREWVWAVRPNNGLMLQGDESPNAGRER
                     IFFAINAQNGLHPRLVVTYQIDTTAPSSIVNPLGQWERATFRVAWSGQDNSGGSGLRH
                     FDVQFRVNGGAWQNWQTATTATSANFTGVNAATYDFRVRATDWAGNAQPFPNNPQATT
                     RVDSMPPNSVVNPLPQYTFSDSFLVSWSGLDPQPGSGIARYEVQYQVNGGGWQMFVTD
                     TTTTSATFRNAQVGATYGFRVRALDWVGNVEPFSPQAQAQTTISGGDPVAAIVPFSTR
                     VTQSNTFVVQWQGMPVP"
     gene            1708..3444
                     /locus_tag="PK829_03640"
     CDS             1708..3444
                     /locus_tag="PK829_03640"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOU40342.1"
                     /translation="MNFWTGLILGIIVGWLVEWIIDWLFWRRDVVAEEEALITGQRAA
                     ELELECQDKLAAAELDYHERLAAVEAEWRERLNLNENQWQSQFAAMDVDREALRAPLD
                     MASEADIVAEDLALDEAALSDFAVDEAFIDEGIMTADAADTSVSFADEMGAAVGAATM
                     AEFDTTTGAPAPEAIEVVAEESAGTRDFLSADMPSLYSDEDSAGAPGDDLTRVRGIGP
                     RYAALLADNGITTYEDLAAANPEHLEAILKPGPMQRINFASWSAQATALATTRRAPSG
                     DDLTKLEGIGPMYAERLRNGGIATFAQLAEADENTLATIISAPAWRRINFASWVEQAR
                     LAAAGDTAGLQALQSRLYRREGDNLGLIRGLGDRSVAGLRAAGLDSFAALAAATPEQV
                     EAAVRAGGGRGGDYAAWIEEAGHRAAGRRVARAPRRAKAAVVACPQDLSAVPGIGTTY
                     EDRLYAAGIGSYWELAELSGDELLAILAAPPGRVDAGSIKAEAMRLAAETNSIGRGWS
                     GAPPDNFEALGGIGEIYERRLYDAGICTFEALAATPPERLADICQAPALRLADYAGWI
                     AHAAELAAARSS"
     gene            3448..5208
                     /locus_tag="PK829_03645"
     CDS             3448..5208
                     /locus_tag="PK829_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOU40343.1"
                     /translation="MRRYRTAEEIRRYDLMKLGVLLLLILLLILTWLATRDNGALDLT
                     GAAEPTAVAEPTLEREPGTMPAPTLALPAIDVPVGQLAPGAVTLSGHAGAGAQVAVLA
                     DGLPLGLATASVDGAWALTADLPAGQHTLVAQTIDNVGTIVAMSAPLTITVGEAVLGE
                     ATGQTQPPIFQPLTDTWALAGTAAPGAAIVILANGAPVAETTADAAGAWALAVPGASV
                     AGDVIVQSTDAAGATASAPLTIGARPPSVAAPGQAAAPAAGAVLIVPVGPGVWTGQAA
                     PGTQIEAIVDDQSAGATSADAQGNWSLSLNLPAGQHTLHFNSLDPAGVLLATGSALPV
                     TAGEGEVIAATPEGAAPTTPAETPVAGAAGTIAAVLRARPEFATLVRAIDAAGLADAL
                     AQPGPFTVFAPTEDAFTALPQSVVDALLANPEALAAVLRYHVAQGRYTADQLRTAQPS
                     TIDGRLLTITAQPGGQAVNGALMTATDIAAGNGIIHAIDRILLPPLAAGVRPPVIDES
                     GVPSFVGPRLTVVGSAEPGRTIRVELNGEAFGDAVVVDANGGWQMSGDVSAAEYRIVA
                     FMLNGTTLEAFSRPVALTVR"
     gene            5301..6173
                     /locus_tag="PK829_03650"
     CDS             5301..6173
                     /locus_tag="PK829_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40344.1"
                     /translation="MGDDLMGMLSEPGEPDENDPTSQDERGLGSLLGGLMGGGAESAG
                     GGDLLGSLLGGLMGGGAAGSGQSSTAGSGDMGDLLGGLMGGGAAGSGQSAAQGEGGLG
                     DLLGGLMGGGSSGGLGGLLGGLLGGGGAGTFGGGGGPMLPFAETLAEKLHISPQMANM
                     LIMGAIGLLTASAAKKQSGRSVGVADLTSPDYIRSSGVASRLSSQMGISEDDAVAGLQ
                     EAMGLMAAHSGMATAPRPAAAKKRTAKKAGDATTTPATNTNKPTRKTTRTTPKKSSKS
                     ETGPDFMGLLDDPQ"
     gene            6264..6638
                     /gene="mscL"
                     /locus_tag="PK829_03655"
     CDS             6264..6638
                     /gene="mscL"
                     /locus_tag="PK829_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOU40345.1"
                     /translation="MWKEFRDFINRGNVIDLAVAVILGGAFTAIVNSLVADIIMPIIG
                     VLLGGVDFTALAITVGDANITYGNFIQAIINFLLIAFVVFLIVRSLNRMKKPAPPAAP
                     PGPTNEEVLLMEIRDLLRAQQR"
     gene            6732..7742
                     /locus_tag="PK829_03660"
     CDS             6732..7742
                     /locus_tag="PK829_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40346.1"
                     /translation="MSIFRRIIGLLMLLVGIAGLVIAGAGAYFASQAIDTVFGGLNSA
                     VDTLGTTVDTSTASLENVKATLVETGNTLETVSATASNAAVTLSDTQPLLERMTTLTT
                     DTLPASLDAVNAAVPNLAGIAGSIDATLTRLSDFQFQRTIATVPISFDLGIDYAPEEP
                     FDQAVLQIGESLVDVPDQLRAMEDSLQTTVTNLGNISGNIEDLSGNITGINTTVQQYI
                     PLLDQYIGVLNQTAASLENTRAQLNANLSTIKWVATGLMAWFALYQIVPLYVGYRMLS
                     DKVVEGTFEERVDALDDRPATYAAAKVERAEDAAERAEDAAHAARDAAEDAADETAKA
                     SS"
     gene            7779..>8758
                     /locus_tag="PK829_03665"
     CDS             7779..>8758
                     /locus_tag="PK829_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40347.1"
                     /translation="MSTYHEGDEANRRRVIAAGVILALQIIGLLWLLLFARDRFALGA
                     GQEPPATAAPTAVVTALATAVAAAESEPTATPEDNSAATAAAADAAATADADAAVAAG
                     LTATDAGVVAPEDVHLDTSGLPAAPDWLAQLVEGSAPDATAGTAGMPPHLLLTFPDAQ
                     EPALELTTPDLSRPQVRIVPIAALLAWLQARGDTAGQNALQDLQSLLADPPPADEAVV
                     PPLPFSDLRQPLVARVVFAQFGGGHGVGYVAHMTGDKATPVTADSGLIYVYQGLTDDG
                     RNYVFMSWPMDAAFLGEDADAAATAVAADADAYYDTVAAEIATADDANMTT"
BASE COUNT         1442 a         2981 c         2989 g         1346 t
ORIGIN      
        1 ggcacgggca taccctgcca ctggaccaca aaggtgttgc tctgcgttac ccgggtcgag
       61 aagggtacaa tggccgcgac cgggtcgccg ccggagatgg ttgtctgcgc ctgagcctgg
      121 gggctaaacg gctcgacgtt gcccacccag tcgagagcgc ggacgcggaa gccataggtg
      181 gcccccacct gggcgtttct gaaggtggcc gaggtcgtcg tcgtgtcggt gacgaacatc
      241 tgccagccgc cgccgttgac ctggtactgc acctcatagc gggcgatgcc gctgcccggt
      301 tgtgggtcga ggccgctcca ggagacgagg aaggaatcgc tgaaggtgta ctgcggcagc
      361 ggattgacga ccgagttggg cggcatgctg tcgacgcggg tggtcgcctg cgggttgttg
      421 gggaacggct gcgcgttgcc ggcccagtcc gtggcccgga cgcggaagtc ataggtggct
      481 gcgttgacgc cggtgaagtt ggcggaggtt gccgtagtgg ccgtctgcca gttctgccac
      541 gcgccgccat tgacgcggaa ctgcacgtcg aagtggcgca agccggagcc gccgctgttg
      601 tcttgcccgc tccaggcgac gcggaacgtc gcccgctccc actggccgag cggattaacg
      661 atcgacgacg gcgcggtggt gtcgatctgg taggttacaa ccaggcgcgg gtgaaggccg
      721 ttctgggcgt tgatggcgaa gaagatacgc tcgcggccgg cattgggcga ctcatcgccc
      781 tgaagcataa ggccattgtt tgggcgcacg gcccataccc attcgcgcac cggcgcggtg
      841 acattggcct cgacccagcc agtggcggcc ggaatctggc ccacgccgat ctcggctccc
      901 cactgcgggt tgaagttgtt ccaggtcagg atcgttggat cccaagccgt ggataggaaa
      961 cgagccagga tgccgaaggg ggcgtcattg gttggggcaa acccggcgac aaaggccctg
     1021 agagtggcgt tctgcacaac ggcgttgcgc ggaatactgc tgaggttgaa gaacaggaag
     1081 ccccgctgcg cgccattgat ggccgagtta aagccgacac ggatgaagct gtcggtagag
     1141 aagttggtcg ccgggcggcc ggaagcggtg aaggtgtccg cgccaacggg aatctcaatg
     1201 gtcacggtgg tggcgtcccc gtcgcgggtc tcgctgagga tgaccggctc cagttgcagt
     1261 tccgtgtctg gaatgtcagt gggcgcgtcg ggagtgccct gcgcgccgac agagacagtt
     1321 gttagggcga ccagtagcac caggaccagc agcgccagcg cgcgccggcc cgcgccggag
     1381 ggggatacaa agttggtcat atgtgtttct ccttacttcc catcacgtac agacagacaa
     1441 ccattgctca ttataatatg gacaattgtc gcaagtcaat tgacgtagag atgatagtta
     1501 gtggcgccat gctcgcccct ccgccccacg ccgatctgac aggcgggcgg ccacgcatgg
     1561 gattgccacc agagaacgac tggccgccta cctgtcaggt cgatggtcgc gagacgactt
     1621 tggaaaagcc ctgagcatta ccacatgccg attcacaact ttccgctata cttttctgtg
     1681 gaaagtctgt tccacgggag gtgctgcatg aacttctgga ccggtctcat tctggggatc
     1741 attgttggct ggctggtcga gtggatcatc gattggctct tctggcggcg cgatgtggtc
     1801 gcggaggaag aagcccttat caccggccag cgcgcggccg agctagagct ggaatgccag
     1861 gacaaactgg ccgctgctga gttggattac cacgagcgtc tggcggccgt tgaagccgaa
     1921 tggcgcgagc gcctgaacct caacgagaac cagtggcaaa gccagttcgc cgctatggac
     1981 gtcgaccgtg aggccctgcg cgcgccattg gacatggcct ctgaagccga catcgtcgcc
     2041 gaagacctgg ccctcgacga agccgccctc agtgacttcg ccgtcgatga ggccttcatt
     2101 gacgaaggga tcatgaccgc tgacgctgcc gacacgagcg tttccttcgc cgacgagatg
     2161 ggcgcggctg tcggcgcggc gacgatggcc gagttcgata ccacgaccgg cgcgcccgcg
     2221 cccgaggcca ttgaggttgt cgccgaggaa tccgccggca cgcgcgactt cctcagcgcg
     2281 gatatgccct cgctctatag cgacgaggac tcagccggag cgccgggcga cgacctgacc
     2341 cgcgtgcgcg gcatcggccc acgctatgcg gccctgctgg ccgacaatgg cattaccacc
     2401 tacgaagacc tggccgcggc taacccggaa cacctggagg ctatcctcaa gcccggcccc
     2461 atgcagcgca tcaacttcgc cagctggtcg gcccaggcca ctgccctggc cacgacacga
     2521 cgggccccat cgggcgacga cctgaccaaa ctggagggca tcggccccat gtatgccgaa
     2581 cggctacgca atggcggcat tgccaccttc gcgcagctgg ccgaggccga tgaaaacacg
     2641 ctggcgacga tcatcagcgc tccggcctgg cggcggatca acttcgccag ctgggtcgag
     2701 caagcgcgcc tggccgccgc cggcgacacg gccggcttgc aggcgttgca gagccgcctc
     2761 tatcgccgcg aaggcgataa cctcggcctg atccgcggcc tgggcgaccg gtccgtggcc
     2821 gggctgcgcg ccgccgggtt ggacagcttc gccgcgctgg ccgcggccac gccggagcag
     2881 gtcgaggccg ccgtccgcgc tggcggtggc cggggcggcg actacgccgc ctggatcgag
     2941 gaggccggcc accgcgccgc cggccgtcgc gtcgctcgcg ccccgcgccg ggccaaagcc
     3001 gctgtcgtcg cctgcccgca agacctgtcg gccgtgcccg gcattggcac gacctatgag
     3061 gatcgcctct acgccgccgg catcggcagc tattgggagc tggccgaact gtcaggcgac
     3121 gaactgctgg ccattctggc cgcgccgccc ggccgggtgg acgccggcag tattaaggcc
     3181 gaggccatgc ggctggccgc cgagaccaac agtatcggcc gcggctggag cggcgcgccg
     3241 cccgacaact tcgaggcgct gggcggcatc ggcgagatct acgagcgccg cctctatgac
     3301 gccggcatct gcacctttga ggcgttggcc gcgacgccgc cggagcggct ggccgacatc
     3361 tgccaggccc cggcgctgcg cctggccgat tatgccggtt ggatcgccca tgccgccgag
     3421 ttggccgcgg cgaggagcag ctaggccatg agacgctatc gcaccgccga agaaattcgg
     3481 cgctatgacc tgatgaagct gggcgtgctg ctgctgctca tcctgctgct catcctgacc
     3541 tggctggcca cccgcgacaa cggcgcgctc gacctgaccg gcgcggccga accgacagcc
     3601 gtggccgaac cgacgctgga gcgcgagccg ggcacaatgc ccgcgccgac gctggccctg
     3661 cccgccattg atgtgcccgt cgggcagctg gccccggggg cggtgacgtt gagcggccac
     3721 gcgggggcgg gggcccaggt ggccgtgctg gccgatggct tgcctctggg gctggccact
     3781 gccagcgtcg acggtgcttg ggcgctgacg gccgatctgc ccgccgggca acacaccctc
     3841 gtcgcccaga caatcgacaa tgtcggcacg atcgtcgcca tgtccgcgcc cttgaccatc
     3901 actgtgggcg aggcggtgct gggcgaggcg accggccaaa cgcagccgcc catcttccag
     3961 ccgctcacgg acacgtgggc gctggcgggc acggccgcgc cgggcgcggc gattgtcatc
     4021 ctggccaacg gcgcgcccgt ggccgagacg acggccgacg ccgccggcgc gtgggcgctg
     4081 gcagtgcccg gcgcgagcgt ggccggcgat gtcatcgttc aatcgaccga cgcggccggg
     4141 gccacggcat ccgcgccgct gaccatcggc gcgcgcccgc ccagcgtcgc cgcgcccggc
     4201 caagcagccg cgccggccgc cggggcggtg ctaattgtgc ccgtggggcc gggcgtctgg
     4261 acggggcagg ccgcgccagg cacgcagatt gaagccatcg tcgacgacca gagcgccggc
     4321 gctaccagtg ccgacgccca gggcaactgg tctctgtcgc tcaatctgcc cgccgggcag
     4381 cacactttgc atttcaacag tctcgatccg gccggcgtct tactggccac cgggtcggcg
     4441 ctgccggtca cagccggcga aggcgaggtc attgccgcca cgcctgaggg cgcggccccc
     4501 acgaccccgg cggagacgcc ggtcgccgga gcggccggca ccatcgccgc cgtgctgcgc
     4561 gcccggccgg aattcgctac cctggtgcgt gccatcgacg ccgccgggct ggccgacgcc
     4621 ctggcccagc cggggccatt caccgtcttc gcgccgacgg aagacgcctt cacggccctg
     4681 ccgcagagcg ttgtcgacgc gctgctggcc aatccggagg cgctggccgc cgtgctgcgc
     4741 tatcacgtgg cccagggacg ctacaccgct gaccagctcc gtaccgccca gccgagcacc
     4801 atcgacggcc ggctgctgac gatcacggct cagcccggcg gccaggccgt caacggcgcg
     4861 ctgatgacgg ccaccgacat cgccgccggc aatggcatca tccacgccat cgaccgcatc
     4921 ctgctgccgc cgctggccgc gggcgtgcgt ccgccggtca tcgacgagtc gggcgtgccc
     4981 agcttcgtcg ggccgcggct gacggtggtt ggctcggccg agccgggccg gacgatccgc
     5041 gtcgaactga acggcgaggc gtttggcgac gctgtcgtgg tcgatgccaa cggcggctgg
     5101 cagatgagcg gcgacgtcag cgccgccgag tatcgcatcg tggccttcat gctcaacggt
     5161 acgacgctgg aggctttctc gcggccggtg gctctgaccg tgcgctaggg cggacgaggt
     5221 gccgatcgca actggaatgg gttagctagt gggccggctg cggcggaacg cgcccgcacc
     5281 aggccaagga gcataaaacg atgggtgatg acttgatggg catgttgtcg gagccgggcg
     5341 aaccggacga gaacgacccg accagccagg atgagcgcgg actaggctcg ttgctaggtg
     5401 gcctgatggg cggcggagcc gagagcgccg gtggcggcga tctgctgggc agcctgctgg
     5461 gcgggctgat gggcggcggc gcggccggca gcggccaaag ctcgaccgcc ggcagcggcg
     5521 acatgggcga cctgctgggt gggctgatgg gcggcggcgc ggccggcagc ggccagagcg
     5581 cggctcaggg cgaaggcggc ctgggcgacc tgctgggtgg gctgatgggc ggcgggtcgt
     5641 ccggcggtct gggcggtttg ctcggtggcc ttttgggcgg cggcggggcc ggcactttcg
     5701 gcggcggcgg cggcccgatg ctgcctttcg ccgagacgct ggccgagaag ctgcacatct
     5761 cgccgcagat ggccaatatg cttatcatgg gcgcgatcgg cctgctgaca gcctcggcgg
     5821 ccaagaagca gagcggtcgc agcgtcggcg tggccgacct gaccagcccc gattacatcc
     5881 gctccagcgg cgtcgcgtcg cgcctgtcgt cgcagatggg catcagcgaa gacgatgccg
     5941 tcgccggcct gcaagaggcg atgggcctga tggccgccca cagcggcatg gccaccgcgc
     6001 ccaggccggc cgcggccaag aagcggacgg ccaaaaaggc cggcgacgcc accacgacgc
     6061 cggcgaccaa taccaataag ccgacccgca agacgacgag aacaacaccc aagaaatcct
     6121 caaagtcgga gaccggtccc gactttatgg gtctgctgga cgatccccag tagcgccccc
     6181 ggcgcgagcg cggatcaccg gcggcggcaa atctgctata gcaacccgcc gccggccacc
     6241 agcatgtaga tgtggaggtt catatgtgga aggaatttcg cgacttcatt aatcgtggca
     6301 atgtgatcga cctggccgtc gccgtcattc ttggcggcgc gttcacggcc attgttaact
     6361 ctctggtcgc cgacatcatc atgcccatca tcggcgtgtt gctgggcggc gtcgacttta
     6421 cagcgttggc cattacggtg ggggatgcca atatcaccta tggcaacttc atccaggcca
     6481 tcattaactt cctgctgatc gcctttgtgg tcttcctcat cgtccggtcg ctgaaccgca
     6541 tgaagaagcc cgcgccgcca gcggcgccgc ccggcccgac aaacgaggaa gtgctgctga
     6601 tggagatccg cgacttgctg cgcgcccaac agcgctagac tctggcggcg ctgccgcgcg
     6661 gcgcggccgg tcccgcgtcg cgcggcagcg ccgatgatca gacaactgca cgcccaacct
     6721 ggaggggtac catgtctatc tttcgtcgca tcatcggttt gctcatgctt cttgtgggca
     6781 ttgccggcct ggtcattgcc ggcgcggggg cctacttcgc cagccaggcc atcgacaccg
     6841 ttttcggcgg gctgaattca gccgtcgata cactcggcac gaccgttgac acatccaccg
     6901 ccagcctcga aaacgtcaag gcgacgttgg ttgagactgg taacacgctg gagactgtct
     6961 cagccacagc cagcaacgcc gccgtcaccc tgtcggacac gcagccgctg ttggagcgga
     7021 tgacgaccct gaccaccgac accttgccgg cgagcctcga cgccgtgaac gccgccgtgc
     7081 ccaacctggc cggcatcgcc ggcagcatcg atgccacgct gacccggctc agcgacttcc
     7141 agttccagcg caccatcgcc accgtgccca tcagctttga cctgggcatc gactacgcgc
     7201 cggaggagcc gttcgaccag gccgtgctgc agatcggcga gagcctcgtc gacgtgcccg
     7261 atcagctacg cgcgatggaa gacagcttgc agacgacggt aaccaacctc ggcaacatca
     7321 gcgggaacat cgaggatttg tcgggcaaca tcaccggcat caacacgacc gtccaacagt
     7381 atatcccctt gctcgatcag tacatcggcg tcctcaacca gacggccgcc tcgctggaaa
     7441 acacccgcgc gcagctcaac gccaacctga gcaccatcaa gtgggtggcc acgggtctga
     7501 tggcctggtt tgccctctac cagatcgtgc cgctctacgt cggctaccgc atgctgagcg
     7561 acaaggttgt cgagggcact tttgaggagc gtgttgatgc gttggacgac cgcccggcca
     7621 cctatgccgc ggcgaaggtc gagcgggccg aggacgcggc cgagcgggcc gaggacgccg
     7681 cccacgccgc ccgcgacgcg gccgaagacg cggccgacga gaccgcgaag gcaagttcct
     7741 agcgccagac cgttgccagg ccgaggagga gaagagccat gtcaacctat catgaaggag
     7801 acgaggccaa ccgccgccgc gtcatcgccg ccggggtcat cctggccctt caaatcatcg
     7861 gcttgctctg gctgctcttg tttgctcgcg accgctttgc cctgggcgcg gggcaggagc
     7921 cgccagccac agcggccccc acggccgtgg tcactgcctt ggccaccgct gtggccgcgg
     7981 ccgagagcga gccaacggcc acgccggagg acaatagcgc ggccacagcc gccgcggccg
     8041 acgccgcggc cacagccgac gccgacgccg ccgttgccgc gggcctgacc gccaccgacg
     8101 caggcgttgt tgccccggag gacgtccacc ttgacacctc gggcctgccg gccgcgccag
     8161 actggctggc ccaactggtc gaaggctccg cgcccgacgc cacggcgggc acagccggca
     8221 tgccgccgca cctgttgctg accttccccg atgcccagga gccggcgctg gagctgacca
     8281 cgcccgatct gagccggccg caggtgcgca tcgtgcccat cgccgccctg ctggcctggt
     8341 tgcaggcgcg cggcgacacc gccgggcaga acgcgctgca agacctgcaa tcgctgctgg
     8401 ccgacccgcc gccggccgat gaggccgtcg tgccgccgct gcctttcagt gatctgcgcc
     8461 aaccgctggt tgcgcgcgtc gtctttgccc agttcggcgg cggccacggc gtcggctacg
     8521 tcgcccacat gaccggcgac aaggccacgc cggtgacagc cgattcgggc ctgatctacg
     8581 tctaccaggg cctgaccgac gacggccgca attacgtctt catgagttgg cccatggacg
     8641 ccgccttcct gggtgaggat gccgatgccg ccgcgacagc cgtggccgcc gacgcggacg
     8701 cctactacga cactgtcgcc gcggagatcg cgacggccga cgacgcgaac atgaccac
//
LOCUS       DAOSMQ010000069         8615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8153_length_8615_cov_4.525000, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000069 DAOSMQ010000000
VERSION     DAOSMQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8153_length_8615_cov_4.525000"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..603
                     /locus_tag="PK829_03670"
     CDS             130..603
                     /locus_tag="PK829_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HOU40348.1"
                     /translation="MKSNESRLWPPLFVAWLIALLATAGALFLGEVMGMVPCVLCWYQ
                     RILMYPLAVVTLVGALKHDEYLPTYVLPLSITGILVSGYHVLLEKGVFPPSTTCAADV
                     PCNISYVNYFGFITIAVMALTAFVLITAIMIATRSAYRRDVSGQLPLPTSEDFSS"
     gene            600..1040
                     /locus_tag="PK829_03675"
     CDS             600..1040
                     /locus_tag="PK829_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOU40349.1"
                     /translation="MTGGWRKARAWLLGLVGVVFLAGCGGAGSAAEPDLGDPATLGLR
                     TFAQWCAPCHGAEGEGHINALEAPALNAAGDSYLLTDAEILDGIVKGGTQEGSQMQPL
                     GQFLTVEQQMAALYYVHTLWSDEQRATHDAAGGHTPPTPTAEGN"
     gene            complement(1214..3514)
                     /locus_tag="PK829_03680"
     CDS             complement(1214..3514)
                     /locus_tag="PK829_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40350.1"
                     /translation="MLSSRRWASRFVLPAILLLAAAFRLYRLLDFPPGFHFDEAIDLK
                     IALDVAHGARPLYVPEGWGREALYYYLVAPLLRVIDYNPLALRVTAVVCGLGVLVAAY
                     GLARRWRGPLAGWLTAAWLAVAMWPVWAARFGVRHISLALMLGLAAWAFWRAFGEITN
                     DEVGIRNEEGERDAASAPNPDSGLQVPGSRFQGRGFFPRRLEPGPPSSSPGTWPLEPG
                     PSSAAPNPYPLAPIPFFLAGLLLGLTAYTYQPARFVPLLFGGFGVYLALFHRRRPWTY
                     RRGWLVWGLTAALVALPLVVILTQATPIETGERAFSIEPLTELLAGNPRPLLDNAVAL
                     AGVFTVAGDPLKSYNLPGRPLFVPRWTGVFFYAGLALALWRWRRPFYAFALLWLATGL
                     LGSLVTISAPNFNRMVAAQFPVFFLAGSAAAEAIGWAGRRARWAGAAAALLVVVALGW
                     TARDTARDLFHVWPEQFADAHPLNREIAAVARYLESSDDPRPVVISSRDIEDEDPYVV
                     SVSLDRDVARRWVDTSQALALPAGAAEARLFVTADRWIDPALLEWVGEDAPAAGATTA
                     VDTRPAAAGWWAGLGARLWALVQSPLDEAHLPFARHVLRAEEWPALETPALSLPPESS
                     YMGITYPIPFCACAPGDDPAPALSLLGVRAIPARPGEELTLLTTWATGEARFISLAMF
                     VHLLDASGAIVAQDDGLGYPPHTWQPGDRFVQVARLPLPPDLPPGDYTLQFGLYDRAD
                     GRRWPVLTPDGQPTADRLLLPVHVGE"
     gene            3618..3857
                     /locus_tag="PK829_03685"
     CDS             3618..3857
                     /locus_tag="PK829_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOU40351.1"
                     /translation="MLTKTISSTEAQNNFGRILDDVTQNSVRYVIRRRSASQAILLSL
                     AELERLLSATEHERQDVGRVIRELSPIYDLGQSVE"
     gene            3872..4816
                     /locus_tag="PK829_03690"
     CDS             3872..4816
                     /locus_tag="PK829_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU40352.1"
                     /translation="MDELDWRQPFAFDYPTKAWSSSLAVQADCMEWLRRVPENSLHAI
                     VTDPPYGVKEYDFDQLEKRGNGNGGIWRIPPSFDGHQRSPLPRFTALSEAERQQLREF
                     FRAWAQLAARVLRPGGHLFLASNAFLAQMIFGIIAEEGLEFRGQIIRLVQTLRGGDRP
                     KNAEKEFPEVCTFPRGGYEPWGIFRKPLPAGMTVRDCLLAYETGGLRRKPDGRPFEDV
                     IVSERTPQRERAIADHPSLKPQSFLRQIVYAALPLGRGIVLDPFMGSGSTLAAAEFVH
                     YRVIGIERHPDYFRLAEEAIPALAKLSIKQDQLELFPS"
     gene            complement(4818..5456)
                     /locus_tag="PK829_03695"
     CDS             complement(4818..5456)
                     /locus_tag="PK829_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40353.1"
                     /translation="MDAQDDFSEEQACTAMPVQRDKLNTLSVLPYGLNAEHIYLAMSD
                     FIRFLGLVNRQLFTQQIPRLESFLMPANFSSIVGEFLNMTIPRYCPTLAKNRFHNGHP
                     DLIPAGYYPDDAVQYAHQGIEVKASRYAAGWQGHNPETVWLVVFQFESNSASERMARP
                     RPFRFRAVYAAQLELEDWRFSGRSATSRRTITASVTNSGRLKMQANWIYRDL"
     gene            5754..6518
                     /locus_tag="PK829_03700"
     CDS             5754..6518
                     /locus_tag="PK829_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU40354.1"
                     /translation="MELNTTIDRQSYIPLYVQVKDSLKELIDGGGRAPGEQLPGEPEL
                     CRLYDVSRTVIRQALRDLELEGLIVREKGVGTFVAEPKLREGLFQELTGFYGDMANKG
                     RPPVSQMLTQEVIAAPRKIAAYLRLRPGAAVVHIDRLRFVAEEPIVLVATYLPAARCP
                     GLEAVDFTRRSLYEYLESAYGLTIARGRRTVEAVPAGEYEARLLGVKKGAPLIQLDSV
                     SFLSDGTPIEYYHALHRGDRSRFEVELLRGILAVHE"
     gene            6534..7394
                     /locus_tag="PK829_03705"
     CDS             6534..7394
                     /locus_tag="PK829_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU40355.1"
                     /translation="MKVGIDSYCFHRFFGEVYPHQTPPPFQMTVFDFLDFARELAVDG
                     VSLESCFFPSYDKAFFADLKARLDEYGFDRVYAWGHPDGLEAGKNRAEFESMLKHIEY
                     APLIGAPVMRIVASSLMFRFEPHGPQIDILVDWLKEAVKVAEANGVKLAVENHIDYTS
                     DECVELLDRVDSPYLGLNLDTGNFLRLLDDPVDGARKLANRVLATHIKDLKPVRGVNA
                     KEWYFFSSTPVGDGLVDNQKLAQILHEANYQGFLAVETDSLHPDYENQEHWAVEKSVW
                     MLKRIARNVR"
     gene            7409..8566
                     /locus_tag="PK829_03710"
     CDS             7409..8566
                     /locus_tag="PK829_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU40356.1"
                     /translation="MKTMNVALIGTKFMGKAHSNAWANAPRFFDMGIRPVLAVAVGTD
                     MAGAQRLADTWGWAEASTDWREVVGRADIDIVDISTPTYLHHDMAVAAAEAGKHVFLE
                     KPFALSTRQAEEMLAAAEKAGIVHYVNHNYRRAPAVRLARRLIDDGFIGRLFHWRSAY
                     LQDWIVDPDFPLTWHLRAETAGYGAHGDLGSHSVDLARYLVGDITAVTGLMANFITER
                     PLPGAGAATFSAGAGAGEATGAVSVDDASFFVAEFANGALGSFEVSRFAPGRKNYNTF
                     EIYGSEGSLVFNLERMNELQVFSRNDPAYAQGFRTVLATEAGQHDYIANWWPPGHMIG
                     YEHEFHHAVVDFMHAIETGEEIRPNFRDGLAEMQVLDAAAQSARTGQKVTL"
BASE COUNT         1556 a         2780 c         2848 g         1431 t
ORIGIN      
        1 tgctgtgctt ggggttgtcg cactgattgg ggttgtctac ctgaattacc gggattggca
       61 gcgcaagcgt gagcgccggg aagagcggcg gcaaaagcgc catgaagcca agcgcaggaa
      121 gaagaactga tgaagagcaa tgagtctcgg ctctggccgc ctcttttcgt ggcgtggctg
      181 atcgcactgc tggcgaccgc cggtgcgctg ttcctcggcg aggtcatggg catggtgccc
      241 tgtgtgctgt gctggtatca gcgcattctg atgtacccgc tggcggtggt gacgctcgtg
      301 ggcgcgctga agcacgatga atacctgccg acatacgtgc tgcccctgtc gatcaccggc
      361 atcctggtgt cgggttacca cgtgctgctg gagaagggcg tctttccgcc ctcgacgacg
      421 tgcgccgccg acgtaccctg caatatcagc tacgtcaact acttcggctt catcactatt
      481 gccgtcatgg ccctgaccgc ctttgtgctg attacggcca tcatgatcgc cacgcgctcg
      541 gcctaccggc gcgatgtctc cgggcagttg ccgctgccca ccagcgagga cttctcctca
      601 tgaccggtgg ttggcgcaag gcccgcgcct ggttgctggg gctggttggg gttgtgttcc
      661 tggccggttg cggcggcgcg ggcagcgcgg ccgagcctga tctgggcgac ccggccacgc
      721 tggggctgcg cacgttcgcc cagtggtgcg ccccctgcca cggcgcggag ggcgagggcc
      781 acatcaacgc cctggaagct ccggccctga acgccgccgg cgacagttat ctgctgaccg
      841 acgccgagat tcttgatggg atcgtcaagg gcggcacgca ggagggcagc cagatgcagc
      901 cgctgggcca gttcctgacc gtggaacagc agatggcggc gctgtactac gtccacacgc
      961 tctggagcga cgagcagcgg gcgacccacg acgctgccgg ggggcacacg ccgccaacgc
     1021 ccaccgcgga aggaaattag gaattaggaa tgaagaggga gagcgtgacg ctgcttctgc
     1081 gcctaaaccc gactctgggt tacaggttcc agggaagagg gttcttcccc cgccgcctgg
     1141 aacctggccc tcctagctct tcccctggaa cctggcccct agaacctggc ccctcctccg
     1201 ccgcccctac cccctactcc cccacatgca cgggcaatag cagccgatcg gccgtcggct
     1261 gcccgtcggg cgtcagcacc ggccagcggc ggccgtcggc gcggtcgtag aggccgaact
     1321 ggagcgtgta gtcgcccggc ggcaggtcgg gcggcagggg caggcgggcg acctgcacga
     1381 agcggtcgcc gggctgccaa gtgtggggcg gatagccgag gccgtcgtcc tgggccacaa
     1441 tcgcgccgga cgcatccagc aagtggacga acatggccag cgagatgaaa cgcgcctcgc
     1501 ccgttgccca ggtagtcagc agggtcagct cttcgcccgg ccgggccggg atggcgcgca
     1561 caccgagcag cgacagcgcc ggcgcagggt cgtcgccggg ggcgcaggcg cagaagggga
     1621 tggggtaggt gatgcccatg tagctgcttt cgggcggcag cgacagggcg ggcgtctcta
     1681 gcgcgggcca ttcctcggcg cgcaggacgt ggcgggcgaa gggcagatgc gcctcgtcca
     1741 gaggcgattg caccaacgcc cacagccgcg cccccaggcc cgcccaccac ccggcagcgg
     1801 ccgggcgagt gtcaaccgcg gttgtcgctc cggccgccgg cgcgtcttcc ccaacccatt
     1861 ccaacaacgc cgggtcgatc cagcggtcgg cggtgacgaa cagccgcgcc tcggccgcgc
     1921 ccgcgggcag ggccagcgcc tgcgacgtat cgacccagcg acgcgccaca tcgcggtcga
     1981 ggctgacgct gacgacgtag ggatcttcgt cctcgatgtc gcggctgctg atgaccaccg
     2041 gccgggggtc gtcgctgctc tccaggtagc gagccacggc ggcgatctcc cggttgagcg
     2101 ggtgggcgtc ggcgaactgc tccggccaga cgtggaagag gtcgcgggcg gtgtcgcggg
     2161 ccgtccagcc cagggcgacg acaaccagca acgccgccgc cgccccggcc cagcgcgccc
     2221 gccggccggc ccagccgatg gcctcggccg cggctgagcc ggccaggaag aagactggaa
     2281 actgcgccgc gaccatgcgg ttgaagttcg gggcgctgat ggtgaccagc gagccgagca
     2341 ggccggtggc cagccacagc agggcgaagg cgtagaacgg ccgccgccag cgccacaggg
     2401 ccagcgccag cccggcatag aaaaacacgc ccgtccagcg gggcacgaag agcggccggc
     2461 cgggcaggtt gtaggacttg agcgggtcgc cggcgacggt gaagacgccc gccagggcga
     2521 cagcgttgtc gagcagaggg cgcgggttgc cggccagcag ttctgtcagc ggctcgatgg
     2581 agaaagcgcg ctcgcccgtc tcgatgggcg tggcctgggt caggatgacg accagcggca
     2641 gggcgacgag cgcggccgtc agcccccaca ccagccagcc gcgcctgtag gtccacggcc
     2701 ggcggcggtg gaagagagcc aggtagacgc cgaagccgcc gaacagcagt ggcacgaagc
     2761 gcgctggctg gtaggtataa gcggtcagac ccagcagcag gccggcgagg aagaagggga
     2821 taggggctag gggataggga ttaggggcgg cggaggaggg gccaggttct aggggccagg
     2881 ttccagggga agagctagga gggccaggtt ccaggcggcg ggggaagaac cctcttccct
     2941 ggaacctgga acctggaacc tgcaacccag agtcgggatt aggcgcagaa gcagcgtcac
     3001 gctcgccctc ttcattccta attcctactt cgtcattcgt aatttcccca aacgcccgcc
     3061 agaaggccca cgcggccagg ccgagcatca gcgccaggga gatgtggcgg acgccaaagc
     3121 gggcggccca gaccggccac atggccacgg ccagccaggc ggccgtcagc cagccggcca
     3181 gtggcccgcg ccagcgccgc gccaggccat aggcggcgac gagcacgccc agcccgcaaa
     3241 ccacggcggt gacgcgcagg gccagcgggt tatagtcgat gacgcgcagc agcggggcga
     3301 cgaggtagta gtagagcgcc tcgcgccccc agccctccgg cacatagagc ggccgcgccc
     3361 catgcgccac gtcgagggcg atcttcaggt cgatggcctc gtcgaagtgg aagccgggcg
     3421 ggaagtcgag caggcggtag aggcggaagg cggccgccag gaggaggata gcggggagga
     3481 caaagcggct cgcccagcgg cgcgatgaga gcatagaggg aattataggc caattctgaa
     3541 cgaaggatac ttttcttgac ggccagacca gacgcaacta caatttagct aaattagcta
     3601 atcgcgcagg atagaccatg ctgacgaaga ccatctcatc tactgaggcg cagaacaact
     3661 tcggccgaat tcttgatgac gtgactcaaa actccgtgcg ctacgtcatc cggcgtcgca
     3721 gcgcgtcgca ggccatccta ctgagcctgg ccgaactaga aagactattg agcgccacgg
     3781 agcacgaacg tcaggatgtc ggccgtgtga tccgcgaact tagccccatt tacgatctgg
     3841 gccaatcggt agagtgacca ggtgaaccgt catggacgaa cttgattggc gtcagccctt
     3901 tgcttttgac taccccacca aggcgtggag ctccagcctg gccgtgcagg ccgattgcat
     3961 ggagtggctg cgccgtgtgc cggagaacag cctgcacgct attgtgacgg atccgcccta
     4021 cggggtgaag gagtatgact tcgatcagct ggaaaagcgg ggcaacggca acggcggcat
     4081 ctggcgcatc ccaccgtcat ttgacggaca tcagcgctcg ccgttgccgc gttttaccgc
     4141 gctttccgaa gccgagcggc agcagttgcg ggaattcttc cgcgcctggg cgcaactggc
     4201 ggcgcgcgta ttgcgacccg gcggccacct tttcctggcc tcgaatgcct tcctggcgca
     4261 gatgatcttt gggatcatcg ccgaagaggg cctcgaattc cgtgggcaga tcatccggct
     4321 tgtgcagaca ttgcgcggcg gcgacaggcc caagaacgcg gagaaggagt ttccggaggt
     4381 ctgcaccttc cctcgtggcg gctatgagcc gtgggggata ttccgcaagc cgttgccggc
     4441 gggtatgacc gttcgcgatt gtttgctcgc ttatgagacc ggtggcctgc ggcgaaaacc
     4501 ggacggccgg ccgtttgagg atgtcatcgt cagcgagcga accccgcagc gagagcgagc
     4561 gattgccgac catccgagcc tcaagccaca atcgtttcta aggcagatcg tttatgcggc
     4621 gctgcctttg ggccggggga tcgttctcga tccgttcatg gggtcaggat cgactctggc
     4681 cgcggccgag tttgtacact atcgagtcat aggcattgag cgccacccgg actatttcag
     4741 actggctgag gaggccatcc ccgcactggc gaagttgagc atcaagcaag atcagttgga
     4801 actgtttcca tcctagatca tagatcgcgg taaatccagt ttgcctgcat tttcagccgc
     4861 cctgagtttg taacgctggc ggtgattgtc cggcggcttg tggctgaccg gcccgaaaaa
     4921 cgccagtcct ccaattctag ctgtgctgca tagacagccc tgaaccggaa tggccgcggc
     4981 cgtgccatcc tctcgctggc agagttgctt tcaaattgaa agaccacgag ccaaacggtt
     5041 tcagggttgt ggccttgcca accggcggca taacgcgatg ctttcacttc gatcccctga
     5101 tgcgcgtact gcaccgcgtc gtctggatag tagccggcgg gaatgaggtc gggatgccca
     5161 ttgtggaatc ggttcttagc cagtgttggg caatagcgtg ggatagtcat atttaaaaac
     5221 tcaccgacga tactgctgaa gttggctggc atgagaaagc tctccagcct gggtatctgc
     5281 tgcgtgaata gctggcgatt gacaaggccc aggaagcgga tgaagtcgct catggccaga
     5341 tagatgtgct cagcgttcaa cccataaggc aacacgctga gtgtgttcag cttgtcgcgc
     5401 tgcaccggca tcgctgtgca ggcctgttct tcactgaagt cgtcttgagc atccactcac
     5461 tcgattatag gcaaatgctg cgccagcaac gacactcatt ttcatttggc gcgtgctcat
     5521 attgacaacc ccgaatcgct atgctactct ttccctgtaa taacaggatg acagcataac
     5581 agggaacagg cccacgccgg cgatcgcggg tggccacagg cgacttcggt ggctcacccc
     5641 gctcatctgt tttcctcccc actatcgtgg ctgcctccgt ttcgccggcc gggcctcccc
     5701 tgtctttcca cgcgaacccg ccggcgcggc gggtcgttgg ccgggtagca ttcatggaac
     5761 tcaacacgac catcgaccgg cagagctaca tccccctgta tgtccaggtt aaagacagcc
     5821 tcaaggagct gatcgatggc ggcggccgcg cccccggcga gcagttgccc ggcgagccgg
     5881 agttgtgccg cctctacgac gtcagccgca ccgtcatccg ccaggccctg cgcgacctgg
     5941 agctggaagg actgatcgtg cgcgagaagg gcgtgggcac cttcgtggcc gagccgaagc
     6001 tgcgcgaggg gctgttccag gagttgaccg gcttctatgg cgacatggcc aataagggcc
     6061 ggccgcccgt ctcgcagatg ctgacccagg aagtcatcgc cgcgccgcgc aagatcgccg
     6121 cttatctgcg cctgcggccg ggcgcggccg tcgtccacat cgaccggctg cgcttcgtcg
     6181 ctgaggagcc cattgtcctg gtggctacct acctgcccgc ggcccgctgc cccggcctgg
     6241 aagcagtcga tttcacccgc cgttcgctgt atgagtacct ggaatctgcc tatggcctga
     6301 ccatcgcccg cggccggcgc acggtcgaag ctgtgcccgc cggcgagtac gaggcccggc
     6361 tgctgggcgt caagaagggc gcgcccctca tccagttgga tagcgtcagc ttcctcagcg
     6421 acggcacgcc catcgaatac taccatgccc tccaccgcgg cgaccgctcg cggttcgagg
     6481 tggagctgtt gcgcggcatt ttggccgtcc acgaatgaag aggagttttc cccatgaaag
     6541 tcggcattga cagctactgc ttccatcgtt tcttcggcga ggtgtacccc caccagacgc
     6601 cgccgccgtt ccagatgacc gtcttcgact tcctcgactt cgccagagag ctggccgtcg
     6661 atggcgtttc gctggagtcg tgcttcttcc cgtcctatga caaggcgttc tttgccgacc
     6721 tgaaggcgcg gctggacgag tacggcttcg accgcgtcta cgcctggggc caccccgacg
     6781 gcctggaggc gggcaagaac cgcgccgagt tcgagtcgat gctcaaacac atcgagtacg
     6841 ccccgctcat cggcgcgccg gtcatgcgca ttgtcgccag cagcctcatg ttccgcttcg
     6901 agccgcacgg cccgcagatc gatatcctgg tcgattggct gaaggaggcc gtcaaggtgg
     6961 ccgaggccaa cggcgttaag ctggccgtgg agaaccacat cgactacacc agcgatgagt
     7021 gcgttgagtt gctcgaccgc gtcgattcgc cctacctggg gctgaatctg gacacgggca
     7081 acttcctgcg tctgctcgat gacccggtcg atggcgcgcg caagctggcc aaccgcgtcc
     7141 tggccaccca catcaaggac ctgaagcccg tccgcggcgt caacgccaag gagtggtact
     7201 tcttctcgtc cacgcccgtc ggcgacggcc tggtggacaa ccagaagctg gcccagatcc
     7261 tccacgaagc caactaccag ggcttcctgg ccgtcgagac cgactcgctg cacccggact
     7321 acgagaacca ggagcactgg gcggtcgaga agagcgtgtg gatgctgaag cgcatcgccc
     7381 gcaacgtgcg ttagcgcgag gagccaccat gaaaacgatg aacgtcgccc tcatcggcac
     7441 caagttcatg ggcaaggccc acagcaacgc ctgggccaac gcgccacgct tcttcgacat
     7501 gggcatccgc ccggtgttgg ccgtggccgt gggcacggac atggccggcg cgcaacggct
     7561 ggccgacacc tggggctggg ccgaggcctc taccgactgg cgcgaagtcg tcggccgggc
     7621 ggatatcgac atcgttgaca tcagcacgcc gacctacctc caccacgaca tggccgtggc
     7681 cgcggccgag gccggcaagc atgtcttcct ggagaagccc tttgccctgt ccacgcggca
     7741 ggccgaggag atgctggccg ccgcggaaaa ggcgggcatc gtgcattatg tcaaccacaa
     7801 ctaccgccgc gccccggccg tccgcctggc caggcggctc atcgacgacg gtttcatcgg
     7861 ccgcctcttc cactggcgca gcgcctacct gcaggactgg atcgtcgatc ccgacttccc
     7921 gctgacctgg catctgcgcg cagagacggc cggctatggc gcgcacggcg acctgggcag
     7981 ccacagcgtt gacctggccc gctacctcgt cggcgacatc accgccgtga ccggactgat
     8041 ggccaacttc atcaccgagc ggccgttgcc cggcgcgggc gcggcgacct tcagcgccgg
     8101 cgcgggtgcc ggcgaggcca cgggcgcggt cagcgtcgat gatgcttcct tcttcgtggc
     8161 cgagttcgcc aacggcgcgc tcggctcgtt cgaggtgtcg cgcttcgctc ccggccgcaa
     8221 gaactacaac accttcgaaa tctatggcag cgagggcagc ctcgtcttca acctggagcg
     8281 catgaacgag ctccaggtct tcagccgcaa cgacccggcc tatgcccagg gcttccgcac
     8341 ggtgttggcc accgaagccg ggcagcacga ctacattgcc aactggtggc cgccggggca
     8401 catgatcggc tatgagcacg agttccacca tgccgtggtc gacttcatgc acgccatcga
     8461 gacgggcgag gagattcggc cgaacttccg cgacggcctg gcggagatgc aggtgctgga
     8521 cgcggcggcc cagtcggccc ggacgggcca gaaagtgacc ctgtagcgga gccgggcggg
     8581 ccggcgagag agaaccaatg cagtacggcg cgaac
//
LOCUS       DAOSMQ010000070         8588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8181_length_8588_cov_5.043361, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000070 DAOSMQ010000000
VERSION     DAOSMQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8181_length_8588_cov_5.043361"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PK829_03715"
     CDS             complement(<1..600)
                     /locus_tag="PK829_03715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU40357.1"
                     /translation="MTTIGRYELRGELGQGGMATVYRAYDAMLNREVALKVMAAHLTQ
                     DPSFYRRFEREAKAIATLEHPNIVPVYDYGLTENGQPYLVMRMLRGTLYDRLAAGSMT
                     RQQLLSALAQVASALDYAHGRQVVHRDMKSVNILFDEKANAYVSDFGIAKVVDATTNL
                     TGNAFMGTPAYMSPEQFTGGTLDGRSDQYSLAVVAFEALS"
     gene            934..1272
                     /locus_tag="PK829_03720"
     CDS             934..1272
                     /locus_tag="PK829_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOU40358.1"
                     /translation="MQPRYNLWIEIDGQVGLSLWRAELLRAVARHGSISAAAEQMGIP
                     YRTAWQKIHEMETRLGQKLVATQTGGAHGGGTRLTPAAALYLEQFDRFAHDLDRFIAE
                     AFAANFHLPD"
     gene            1381..2316
                     /locus_tag="PK829_03725"
     CDS             1381..2316
                     /locus_tag="PK829_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU40359.1"
                     /translation="MRRFVLFFLVLSALLVLAACGGGGAAEPTAAPTTAAAVPTEAAT
                     TEPTPEAAAEEPQVLRLATTTSTADSGLLDAILPDFEAAYNARVDVVAVGTGQAIEIG
                     QAGDADVILVHARAREDAFVEEGSGLARYDVMYNDFIIVGPADDPAGVQGMTSAAEAL
                     AAIAAAEAPFASRGDDSGTHTKELSLWEAAGVTPEGDWYNSLGQGMGETLTFANESEA
                     YTLTDRGTYLSMSSTLPNLAIMVGGDSIADNADSALLNPYGVIPVNPDLSANINAELA
                     QQFVDWLISPEVQEQIGQYGVEQFGQPLFYPNAAQ"
     assembly_gap    2414..2513
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2549..3238
                     /locus_tag="PK829_03730"
     CDS             2549..3238
                     /locus_tag="PK829_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40360.1"
                     /translation="MDTLLDALREALRLLTSLDPALVEIIAQTLRVTGSALALAVLIG
                     LPAGAWLGLRERVPAAGCLMPIIYTGMALPPVVVGLFVYLFLSSQGPLGRLDWLFTAR
                     AMVVAQTIIALPLVIGLTLTALRAVDPAVKLQVRSLGATRTQLAWALLLEARLGVLAA
                     IIAAFGGIISEVGAVMLVGGNIEGQTRVLTTAIVLETRRGNFALALALGLILLTLAFV
                     TNWLFHRLSDG"
     gene            3369..4094
                     /locus_tag="PK829_03735"
     CDS             3369..4094
                     /locus_tag="PK829_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU40361.1"
                     /translation="MPSLYKLDHVQKAYAGRVVLDIDELTIGRGEVLALVGPSGSGKS
                     TLLRLLNFLEPPTAGALSFDGQPVTAEPPLAQRRRVTAVFQRPALLRRSVAANIGYGL
                     GLRGQKLPPDALAGWLDRLGLSPLARQSAPRLSAGEAQRLAMARALVLRPDALLLDEP
                     TANLDPYNVGVIERLVAEEQRATGMTVVWVTHDIFQARRVADRAAFLLNGRLVEVAAA
                     ETFFAAPSAPQTAAFLRGELVYM"
     assembly_gap    4218..4317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4556..4831
                     /locus_tag="PK829_03740"
     CDS             4556..4831
                     /locus_tag="PK829_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40362.1"
                     /translation="MKWDDVRQNYPGQWLLVEALDAHSEEGRRVVEELAVIDAFDDSA
                     AAWLRYRQLHHDAANREFYILHTDREELDITERVWLGVRLVNSPAAA"
     gene            4828..5202
                     /locus_tag="PK829_03745"
     CDS             4828..5202
                     /locus_tag="PK829_03745"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOU40363.1"
                     /translation="MNLSIRYGLPFVTAVVEHDGRTLELDNVLLDTGSAGTLFQVDQL
                     ATIGLRMEPEDLIRRIRGVGGAEFVFSKRVDALKIGGLRVAGFEIEVGAMDYGFALDG
                     IIGLDFLRAVQAKIDLGELTIS"
     gene            complement(5290..8316)
                     /locus_tag="PK829_03750"
     CDS             complement(5290..8316)
                     /locus_tag="PK829_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU40364.1"
                     /translation="MSFTTPAALLLLLVLPAVAWLVWPTQPRRTAARPRPRSGWLGLA
                     LRLLILALLILSLAGAQLVRAVDDLAVVFLVDASDSMSRESAAAAEAFVREAVGDMGP
                     DDRAAVVLFGANALVEQPLRHFGSAGDLPPFASQPGRLNTNLAEALRLSLALLPAGTA
                     RRVVVLSDGAATTGDTPAAARLAAAAGVSIDTVYLPRPAAINEVILRDVVAPARVGQG
                     ETFRLEIAAESTTATTATLRVLGDEAVVYEEEVQLQPGANNFVVRLGAATPAFARYRV
                     QLAPAPAADTFPQNNELAAFTEITGQPRVLLVAAVPAEDAPDEAAQLAAALAASGLTV
                     ERTTPAELSPALDDLADYAGVFLVNVNARDLSLRKMAALQAYVRDLGGGLTAIGGPDS
                     FGMGGYFATPLEEALPVDMQIHDEERFPSVSLVLVIDRSGSMTAEEGGVMKIQLAAEG
                     AVRALELLNPGDEMTLIPVDEAADDIIGPVTTADRDSAIAQMRALGAGGGGIFVRTGL
                     QAAAGVLASSDREVKHIVVLADGADAEEKEGVPELIGELTAQGITVSMVSIGQGPDTP
                     WLRQMAELGNGRFHLTNEAANLPQIFTQETAAIQRSYLVEERFFPAQVASSPILAGIR
                     ETPALYGYVATSAKATAQVALETPRADPLLALWQYGLGRSLAWTSDATGRWAQDWVRW
                     GGFATFWNQAARWTFGNRGAGGLAASVRLDGERARLTVDAQDTGGAFLNDLTLSANVV
                     SPSGETQTVALAPVAPGRYEGAFTPATEGAYFIGVGTTADGQQTAADPAAGGLQTTAG
                     WVLGYSPEYAALSSDPALLASLAAQTGGRALAPAGAGGPGPAVVFDHNLDAAPAGQPL
                     WPWLTLAAALLLPLDVAARRLQLTRREWERIRNVELGMWNRKRGSAEPERERVEGMAT
                     LLQAKERATVGRFAKSPAPTMSDERRVTNAESASSDVDGARPVAPSQPPAADVRLPTA
                     ESRPPTTGEATPATDERQSAAAEETLAARLRRRREG"
BASE COUNT         1348 a         2860 c         2771 g         1409 t
ORIGIN      
        1 gctgagcgcc tcgaaggcga ccacggccag gctatactgg tcggagcggc cgtcaagcgt
       61 cccgccggtg aactgctccg gactcatgta ggccggcgtg cccataaagg cgttgccggt
      121 caggttcgtg gtcgcatcaa cgaccttggc gatgccgaag tcggaaacat aggcgttggc
      181 cttctcgtcg aagaggatgt tgaccgactt catgtcgcgg tggacgacct ggcggccgtg
      241 ggcatagtcg agggccgagg caacctgggc cagggccgac aagagctgct ggcgcgtcat
      301 gctgcccgcg gccagccggt cgtagagcgt gccgcgcagc atgcgcatga ccaggtaggg
      361 ctgcccgttc tcggtcaggc cgtaatcgta aaccgggacg atgttggggt gttccagggt
      421 ggcgatggcc ttggcctcgc gctcgaagcg gcgatagaag gaagggtctt gggtcagatg
      481 cgcggccatg acttttagcg ccacttcgcg gttgagcatc gcgtcatagg cgcgatagac
      541 tgtggccatg ccgccctggc ccagctcgcc gcgtagttcg taacgtccga tggtggtcat
      601 cacccatcct cccgtcttgt aacccgtgtt gactgtagta ggtgagtcaa gtggcgtgcc
      661 ggtcgctggc gtcggaaata gcccaattgc tggtagagga tgcgccagaa cgagaacaac
      721 acgctgagaa tgagcggatt ataggaacct gtggtcaaaa ttacaagtgg atttagggtt
      781 gaatgtcctc tcgcaatagg gcacaattcg cgatatcact aaccaactcg cacctctttg
      841 actgaagccc gatactctca tagtatcatg cgtaggtgag cagtcgggag cgcaatcgct
      901 cccaactgcc ggtcgaagga cacaacgcca cccatgcaac cccgctataa cctctggatc
      961 gagatcgatg gccaggtggg cctgtccctg tggcgggccg agctgttgcg cgctgtcgcc
     1021 cgtcacggct ccatcagcgc cgcggccgag cagatgggca tcccctaccg caccgcctgg
     1081 cagaagatcc acgagatgga gacgcgcctg ggccaaaagc tagtcgcaac gcagaccggc
     1141 ggcgctcatg gcggcggcac gcgcctgacg cctgcggccg ccctctacct ggagcaattc
     1201 gaccgcttcg cccacgacct cgaccgcttc atcgccgaag ccttcgccgc caactttcac
     1261 ctgcccgact gagcgctggc cccaccgttt cgcctcaaaa cgcttgcggc gcgtctatgg
     1321 ccgggtatac tttcatcagt cgttatgatc gttcatcata acgacaagga gaaggagacc
     1381 atgcgtagat ttgtcctatt cttcctcgtt ctgtcggccc tgctcgttct ggccgcgtgc
     1441 ggcgggggcg gggcagccga gccgacggcc gcgcccacga ccgccgccgc agtgcccacc
     1501 gaagcggcca cgacggagcc aaccccggaa gccgccgccg aagagccgca ggtgctacgg
     1561 ctggcgacga cgaccagcac ggctgactcc ggcctgctcg atgccatcct gcccgacttc
     1621 gaggcggcct ataacgcccg cgtcgatgtc gtcgccgtcg gcaccggcca ggccattgag
     1681 atcggccagg cgggcgacgc cgacgtcatc ctcgtccatg cccgcgcgcg cgaagacgcc
     1741 ttcgttgagg aaggttccgg cctggctcgc tacgacgtga tgtacaacga cttcatcatc
     1801 gtcggcccgg ccgacgaccc tgccggcgtg cagggcatga ccagcgccgc cgaggctctg
     1861 gccgccatcg ccgccgccga ggcccccttc gcctcgcgtg gcgacgacag cggcacgcac
     1921 accaaggagc tgagcctgtg ggaagccgcc ggcgtcactc ccgagggcga ttggtacaac
     1981 tcgctcggcc agggcatggg cgagacgctc accttcgcca acgagtccga ggcctacacc
     2041 ctgaccgacc ggggcaccta cctgtccatg agcagcacgc tgcccaatct ggcgatcatg
     2101 gtcggcggcg acagcatcgc cgacaacgcc gattcggccc tgctcaaccc ctacggcgtc
     2161 atccccgtca atccagacct gtcggccaac atcaacgccg agttggccca gcagttcgtc
     2221 gattggctca tctcgccgga ggtgcaggag cagatcggcc agtacggcgt cgagcagttt
     2281 gggcagccgc tgttctatcc gaacgcggcg cagtaagaga aacaagcagt ggtcagtgaa
     2341 cagtggtcag tggtcaatag ccagcggccg tgggtactga ttactggaca ctgcccactg
     2401 gacactgccc actnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnactggac
     2521 actgcccact ggacactgcc cacttcccat ggacaccctg ctcgacgccc tgcgcgaggc
     2581 cctgcgactg ctgaccagcc tggacccggc gctggtggag atcatcgccc agacgctgcg
     2641 ggtgaccggc tcggcgctgg cgctggcggt gctcatcggc ttgccagccg gcgcgtggct
     2701 ggggctgcgc gagcgggtgc cggcggccgg ctgcctgatg ccgatcatct acactggcat
     2761 ggccctgccg ccggtagtcg ttggcctctt tgtctacctg tttctatcca gccaggggcc
     2821 gctggggcgg ctcgactggc tcttcacggc gcgggcaatg gtcgtcgcgc agacgatcat
     2881 cgccctgccc ctggtcattg gcctgaccct gacggcgttg cgcgcggtcg atccggcggt
     2941 caaactgcaa gtacgctccc tgggagcgac gcgaacgcaa ctggcctggg cgctgctgct
     3001 ggaggcgcga ctgggcgttc tggcagccat catcgccgcc tttggcggca tcatctccga
     3061 ggttggcgcg gtcatgctcg ttggcggcaa catcgagggg cagacccgcg tgctgaccac
     3121 agccatcgtc ctggagacgc gccgcggcaa cttcgccctg gcgctggctc tgggccttat
     3181 cttgctaacc ctggcctttg tgaccaactg gctgtttcac cggctgagcg acggatgaag
     3241 agatgctcac gccaaggcgc agaggagcca agacgcaaag aagaatctct attcaattct
     3301 tctttgcgcc ttttgcccct tcgcgccttg gcgtaagcgt cttcccgatc aaacactgac
     3361 cgattgccat gccttccctc tataaactgg atcacgtcca gaaagcctac gctggccgcg
     3421 tcgtcctcga tattgacgag ctgaccatcg gccgcggcga ggtgctggcc ctggttgggc
     3481 cgagcggctc cggcaagagc acgctgctgc ggctgctcaa cttcctggag ccgcccacgg
     3541 ccggggcgct gtcgttcgac gggcagccgg tgacggccga gccgccgctg gcgcaacggc
     3601 ggcgggtgac ggccgtcttc caacgaccgg cgctgctgcg ccgcagcgtg gccgccaaca
     3661 tcggctacgg cctggggctg cgcggccaga agctgccgcc cgacgcgctg gccggctggc
     3721 tcgaccggct ggggctaagc ccgctggccc gccagagcgc gccacgcctg tcggccggcg
     3781 aggcgcaacg gctggccatg gcccgcgctc tcgtcctgcg ccccgacgcg ctgctgctcg
     3841 acgagccgac ggccaatctt gacccctaca acgtcggcgt gatcgagcgg ttggtggccg
     3901 aggagcagcg ggccacgggc atgaccgtcg tctgggtcac ccacgacatt ttccaggcgc
     3961 ggcgcgtggc cgaccgggcg gcgtttctgc tgaacggccg tctcgtcgag gtcgccgcgg
     4021 ccgagacgtt cttcgccgcc ccatccgccc cccagacggc ggcctttctg cgcggcgaac
     4081 tcgtttacat gtagggcgcg acccgacacc atcacgtttg tcccggcgcg ggtcttaccc
     4141 agtcccgcgc cgaccatcct ttgccggtgg agcgaggcag gcggcttttt cccggagccg
     4201 cctgcccgcc cccaccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc
     4321 ggtggagcga ggcaggcggc tttttcccgg agccgcctgc ccgcccccac cagcgcgccg
     4381 tgcatcttgc cgccgttcct acaaacctcc tagaaaccgg gtttctcagc agaaacccgg
     4441 gttctagagc ttggcccgca gcggcggcag gaaggcgtta agggggtggg ccaggtgttc
     4501 cagggcgatg acaagttcag gcaatagcat gatacaatcc cacaagaggc ttacgatgaa
     4561 atgggatgac gtccgacaaa actatccggg ccaatggctg ctagtcgagg cgctcgacgc
     4621 ccattctgag gaggggcgtc gcgtggtgga ggaattggcc gtgattgacg cattcgacga
     4681 ctcggccgcg gcctggctac gctaccgtca attgcaccac gacgcggcca acagggagtt
     4741 ctacatcctc cacaccgatc gggaagaact cgacatcacc gaacgggttt ggctcggcgt
     4801 caggctggtt aattctccgg cggcggcgtg aacctctcca tccgttatgg gcttccgttc
     4861 gtcacggctg tggttgagca cgacggccga acgctggaac tcgacaacgt tctgctcgat
     4921 accggttcgg cggggactct cttccaggtt gaccagctgg cgacgattgg cttgcgcatg
     4981 gagccggaag acctgatccg gcgaatccgg ggcgtcgggg gagctgaatt tgtcttttcc
     5041 aagcgggtcg atgcgttgaa gattggcggg ctacgggttg caggcttcga gatcgaggtt
     5101 ggggcaatgg attacggctt cgcgctagac gggatcattg gcctcgattt tcttcgagcc
     5161 gtgcaagcca agatcgacct cggcgagtta acgatctctt gaagacagct cgcccctccc
     5221 accgcctgcc tgtagagtgt agggcgattt ggcaaatcgt cccacggtaa ggcgatttgc
     5281 caaatcgccc tacccctcgc gccgccttcg caaccgcgcc gccaacgtct cctccgccgc
     5341 ggccgattgc cgctcgtctg ttgctggcgt tgcctcccct gttgttggcg gtcggctttc
     5401 ggccgtcggc agccggacgt cggcggccgg gggctggctt ggcgctaccg gtcgcgcacc
     5461 gtccacatct gaggatgcgg actccgcgtt tgtcactcgt cgctcgtcac tcatcgtggg
     5521 cgcaggcgat ttggcaaatc gccctacagt ggcccgctcc ttggcttgca acagcgtcgc
     5581 cattccctcg acccgctccc gctccggctc cgcgctgccc ctcttccgat tccacattcc
     5641 taattccaca ttcctaattc tctcccactc tcgtcgcgtc aactggagcc gccgcgcggc
     5701 aacatccagc ggcagcagca gcgccgcggc cagtgtcagc cacggccaga ggggctggcc
     5761 ggccggggcg gcgtcgaggt tgtggtcgaa gaccaccgcg gggccagggc cacccgcgcc
     5821 agccggggcc agcgcccgcc cgccggtctg tgccgccagc gaggccagca gcgccgggtc
     5881 gctcgataac gcggcgtact ccggcgaata gcccagcacc cagccggccg tggtttgcag
     5941 accgccggcg gccgggtcag ctgcggtctg ttggccgtcg gctgtcgtcc caacgccgat
     6001 gaagtacgcc ccctccgtcg ccggcgtgaa cgcgccctca tagcggccgg gggccaccgg
     6061 agccagggcc acggtctgcg tctcgcccga cgggctgacg acgttggccg acagcgtcag
     6121 gtcgttcagg aacgcgccgc ccgtgtcctg cgcgtccacg gtcagccgcg cccgctcgcc
     6181 atcgaggcgc accgacgcgg ccagcccgcc cgcgccgcgg ttgccgaacg tccagcgcgc
     6241 ggcctggttc cagaacgtgg caaagccgcc ccaacgcacc cagtcctgcg cccagcggcc
     6301 ggtagcatcg gatgtccacg ccagcgagcg gcccaggcca tattgccaca gggccagcag
     6361 cgggtcggcg cgcggcgtct ccagggccac ctgagccgtg gccttggcgc tggtggccac
     6421 gtagccatag agcgccggcg tctcgcgaat gccggccagg atggggctgc tggcgacctg
     6481 agccgggaag aagcgctcct ccaccaggta gctgcgctgg atggcggccg tctcctgagt
     6541 gaagatctgg ggtaggttgg ccgcctcgtt ggtcaggtgg aagcggccgt tgcctagctc
     6601 ggccatctgc cgcagccagg gcgtgtcggg gccttgccca atgctgacca tcgacacggt
     6661 gatgccctgc gccgtcagct cgccgataag ttcgggcacg ccctccttct cctcggcgtc
     6721 ggccccgtcg gccaggacga cgatatgctt cacctcgcgg tcgctgctgg ccagcacgcc
     6781 ggcggcggcc tgcaggccgg tgcgcacgaa gatgccgcca ccgcccgcgc ccagcgcgcg
     6841 catctgggcg atggcgctgt cgcggtcggc cgtcgtcacc gggccgatga tgtcatcggc
     6901 ggcctcgtct acggggatga gcgtcatctc gtcgccgggg ttgagcagtt ccagcgcccg
     6961 cacggccccc tcggcggcca actggatctt catcaccccg ccctcctcgg cggtcatgct
     7021 gcctgaccgg tcgatgacca gcaccaggct gacggacggg aagcgctcct cgtcgtggat
     7081 ctgcatatcc accggcaagg cctcttccag tggcgtggcg aagtagccgc ccatgccgaa
     7141 gctgtccggc ccgccgatgg ccgtcaggcc gccgcccagg tcgcgtacgt aggcttgcag
     7201 cgcggccatc ttgcgcagcg agaggtcgcg ggcgttgacg ttgaccaaaa agacgccggc
     7261 gtagtcggcc aggtcatcca gcgcgggcga aagctcggcc ggcgtcgtcc gctccacagt
     7321 caggcccgac gccgccaggg ccgcggccag ttgggcggcc tcgtcgggcg cgtcctcggc
     7381 cggcacggcg gcgacaagga gcacccgtgg ctggccagta atctcggtga aggcggccag
     7441 ctcattgttt tggggaaagg tgtcggccgc cggcgcggga gcgagctgca cgcggtagcg
     7501 ggcgaaggcc ggcgtcgccg cccccaggcg gacgacgaag ttgttggccc ctggctgcaa
     7561 ctgcacttct tcctcgtaga ccacggcctc gtcgcccagg acgcgcagcg tcgccgtggt
     7621 ggccgtcgtg ctctccgcgg cgatctccag ccggaacgtc tccccctggc cgacgcgggc
     7681 aggggcgacg acgtcgcgca ggatgacctc attgatggct gccgggcgcg gcaggtagac
     7741 ggtgtcgatg gacacgcccg cggccgcggc cagacgggcg gccgcgggcg tgtcgccggt
     7801 ggtcgccgcg ccgtcgctca ggacgacgac gcgccgcgcg gtgcccgccg gcagcagcgc
     7861 caggctcaac cgcaatgcct cggccagatt ggtgttcagc cgtcccggct gcgaggcgaa
     7921 cggcggcagg tcgccggccg aaccgaagtg gcgcaggggc tgctccacca gcgcgttggc
     7981 cccaaagagg acgacggcgg cgcggtcgtc tggccccatg tcgcccacgg cctcgcgcac
     8041 aaatgcctcg gccgcggcag cgctctcccg gctcatcgag tcagacgcat cgacgagaaa
     8101 gacgacggcc aggtcatcga cggcgcggac gagctgcgcc ccggccaggc tgaggatgag
     8161 cagagccagg ataagcagcc gcagggccag ccccagccag ccggagcgcg gccgtggccg
     8221 ggcggccgtg cgccgcggct gggtcggcca gaccagccag gccaccgcgg gcaagaccag
     8281 cagcagcaac agcgcggccg gggttgtgaa actcattggg ataatcactc tttccaaaat
     8341 ttatcccaac agcaatttgg gaaatagttg ggatatttgt agggcgggtt ggcaacccgc
     8401 cccttcaaag gcggggaagg gcgggttgcc aacccgccct acaagttact tgaggatgta
     8461 gtaggtgccc ttcttggagc cgatcttcag cagtatgccg cggtcaacca gatcggccag
     8521 gtcgaggcgc agtgtctccg gcgacacgtc ggggcaaagc tggcgatact cgcggttggt
     8581 gatgctgc
//
LOCUS       DAOSMQ010000071         8548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8217_length_8548_cov_4.390675, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000071 DAOSMQ010000000
VERSION     DAOSMQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8217_length_8548_cov_4.390675"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PK829_03755"
     CDS             complement(<1..791)
                     /locus_tag="PK829_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOU40365.1"
                     /translation="MPSLNRSEFLAGVRAEVPIVIGVVPFGLIFGAVAVGGENAAGGL
                     APALAQAMSSVVFAGSAQFIGVQLMAGGAASFVLLLTTLVVNLRHLLYSASLAPYVRH
                     LPLRWRLLLAYLLTDEAYAPTIIRYLEVDAPRAAGHWFFLGAGLALWFGGLDSMAGAL
                     SLGTIVLFINSATRFFDPVHELARVLTDVQSAQASTERVMGLLASVPEIRDSDEVRQA
                     IRNHAGCSREGLAIDGLDDRIGTIEFRDVTFAYKQGQQVLEHFNLT"
     gene            complement(809..3802)
                     /locus_tag="PK829_03760"
     CDS             complement(809..3802)
                     /locus_tag="PK829_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOU40366.1"
                     /translation="MIRSRPIRCLCISLGLLALSLTLVACDPGGRLAQLTPQPPVASP
                     VVLGVTAEAPDGASAPPDSVATTAVLPPPAGPTATYDPARPAWTVLYYGGADNDRAAF
                     VWDDLNEMEAGAPSDQVQVVAQVDWPEGGPAGTAESARYHIRADADPQALASEVVATL
                     GETNQGDPQTLADFLTWAVSAYPANRTLLVLGDYGGGWRGCCLDTTAGAPDQTDHLSL
                     TDIDQALAYAQAQTGTRFEVVAFAAGLMSQLDVLQTIQPYAAYAVASPDLVAGASWDF
                     QAVVAQLNSEPLVDGRQLAGDMVTAYVNTQRQLEGDEFASMAAVDLAHIPALSAAVEA
                     LAGALSAEPRLYGELAGFARRGAQVYGRAALADADSIAAVDLSHAAALIAEVSPPGDV
                     QTAATAVSAALTEALVAFDHGQGLAMGRGVALYWPGSVAAVDPLYGGISRLPTWAGYL
                     NAFVGATAGSTTRLTVEPDPRTPAPPVAHIAQPAFLRAEIVAQRVGQVALVAGQEAAD
                     GRYVLRQEEVVAPTAQTLPGGTNTSLWSDGWHESLVVWDATGSYLSDSAGAGDYTPLR
                     AVAPSPLGPQTAARGFFRRAGSDRAAEAAVVFAPGAPASSRLWLTFPADAGARLIGEA
                     RPQAGDTFQPSLLVLAADGTLSTEPGAMLVYDDAPALYRSARPLPAGRYAVGLRVTAL
                     DSSATLATQPVTVDPTGAPEGFRAYVDVTNSMQFLYPAGWLPPATQEGITFTRDVSGT
                     AQLQVRTYPNWAGDLATLQNDVLGTFGQVSVLLQEPVTVGPAPGVEGVRTAYGYDSAE
                     QGPRTGAFVTFVQEGTGTVIDLDGPREAEATTVATLGTIAATWQRLPPRLGFGGEPWS
                     TLNVGEFRLHYPAGFAYEPYNNWHRFAADAQTFVAVRIQPAARTAAEAMSSLLATASE
                     GVAGFAADEPERFFYAGHIWERNDFIYTAADGRPVRGLLLSRQDGETEVAVWAEAPDP
                     TTDLFETTFLPTAASIERIVAPPSG"
     gene            3988..5100
                     /locus_tag="PK829_03765"
     CDS             3988..5100
                     /locus_tag="PK829_03765"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOU40367.1"
                     /translation="MPDDWYSYSGNLHIHTRYSDGEKLHAEIAHDAIAAGLDFIIITD
                     HNVYVTGVEGYYHNDKGRVLLMTGEEVHNVRRQPQASHFLAYGAGRELAPLAADPQAL
                     IDETLSAGGYGFLAHPHEKDLRIIDSPDLGWHDWDIEGFSGLEIWNYMSVFKNTVAAR
                     LEGLRWRNKATESLLALWMTLHPERYMTGADPETLALWDSFLAKGMRITAVGNSDAHG
                     TPMHLGPIHREVYPYEFLFRAVNTHVLTPEPLNGDFDHDRALLLRAIGRGQSWVGYDM
                     PASTAGFRYTVKGTRRGIIGEEMPLGAAGATLQVKTPARCRIRIIRHGSVVAEAERDT
                     NLAYAPSEPGAYRVEGHILYEGQERGWIYSNPIYLR"
     gene            complement(5189..7981)
                     /locus_tag="PK829_03770"
     CDS             complement(5189..7981)
                     /locus_tag="PK829_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU40368.1"
                     /translation="MTSFRSGPVAWRQWAGQRLEERDRPRDRREGLLLALAALFVVAN
                     AATLALTMQDWSWPRLVWPAVWLLVSVAAHMTLRHLKPHRDPFLLPVYALLAGWGLLL
                     QDRLAPNFLGRQTLWYVVATAALLLVAIVPRSLQPLTRYRYVLLVGGLLLVAVTLVFG
                     VNPSGGGAALWLSIPVPFLDVVYFQPSELLKLLLVIFLASYFTEQEPLYRYNRQRAAG
                     NGARPNPARRLLHQLPFLGPLVLMWGFCLLLLVWQQDLGAAGLFFIVFVALLYLATGE
                     RLYVVSGIVLLMAAAVFAFFAFNAVAPRVLTWLNPWPNVSDRAYQVVQSLYAQAAGGI
                     FGRGAGQGFPDYIPVVHSDFALAAIAEEWGLVGSLTVVFAIALVTFRGLRAALLSLQG
                     FRPRTFHAYLAAGLVVLFAIQAMLIIGGVIKLLPLTGITLPFISYGGSSLLISSIMIG
                     LLLYLSAANDGATPTRAYDPRLARRIERLGLALLLGFGVVALTLAYWAAVRSEVLAAR
                     PDNPRLVEAERRVQRGRIVDRNGVVLAESVGETGALTRVYPLSESGPAVGYYSVRFGA
                     AGIERAYDAALRGHSGALWDEVWQAVLHVSHQGRDVRLTLDAEWQRTAAAELAGHVGG
                     LVLLELEPDAETPTAAIRALVSEPDYDPNALAEQFETLAADVRGPLFDRASQGLYQPG
                     LVVQPFLTAAAVDAARIQLGDIVADPFAPVTLDGRVQRCVQRQEETGPTARYTWADMA
                     ALRCPAPLQALGRDMGAAALAEVFDVFGLTTAPALPIATSGSPADIDDPALAAIGQAG
                     LTVTPLQAALATTTLAAGGRRPVLRLVEAVTDASGQWVGQPSALVATTAVRAATAAAV
                     RQTWPRSGDAGELAASSRGFAVSPLSGPDGHHNSWYLGFAPADAPRFVIALVLENEDD
                     IAAAEAIGRAVLAATE"
     gene            complement(7978..>8548)
                     /locus_tag="PK829_03775"
     CDS             complement(7978..>8548)
                     /locus_tag="PK829_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOU40369.1"
                     /translation="VVPDQLFDFTKGARERSFFGNGLVAHVTVAQPFCPHLSAALAEA
                     TEAAGGRLHRGGNIITVEGPRFSTRGESEIFRQWGMSAIGMTTSPEAFLAAEAEISYA
                     CMVHITDYDVWHHSEAPVTVEMVVRVLRANTQLAQAAISALVAGYDDWAGEFPAHSAL
                     KDAIITPAEHISPDVRANLQPLIGKYLPG"
BASE COUNT         1468 a         2883 c         2994 g         1203 t
ORIGIN      
        1 gtcaggttga agtgctccag cacctgctgc ccctgcttgt acgcgaaggt gacgtcgcgg
       61 aactcgatcg tgccgatgcg gtcgtccagg ccgtcgatcg ccaggccctc ccggctgcat
      121 cccgcgtggt tccggatcgc ctggcggacc tcgtcgctgt cgcggatctc cggcacgctg
      181 gccagcaggc ccatcacccg ctcggtcgac gcctgggccg actgcacgtc cgtcagcacc
      241 cgcgccagtt cgtgcacggg gtcgaaaaac cgcgtggcac tgttgatgaa caggacgatc
      301 gtgcccaggc tcagcgcgcc ggccatcgaa tccaggcccc cgaaccacag cgccagcccc
      361 gcgcccagaa agaaccagtg gcccgcggcc cgcggcgcgt ctacttccag atagcggatg
      421 atcgttggcg cgtaggcttc gtcggtcagc aggtaagcca gcagcagccg ccagcgcaac
      481 ggcagatggc ggacgtaggg ggccagcgag gcgctataga gcaagtggcg cagattgacg
      541 accagcgttg tgagcagtag cacgaacgac gccgcccccc cggccatgag ttgcacgccg
      601 ataaattgcg ccgagccggc gaagacgacc gacgacatcg cctgggccag agccggggcc
      661 agccccccag cggcgttctc gccgcccacc gccaccgcgc caaagatcag cccgaacggc
      721 acaacgccga tgacgatggg cacttcggcg cgcacgccgg ccaggaattc gctccgattg
      781 agggaaggca tggcgagggg cggacgattc agccggacgg cggggcgacg atgcgctcga
      841 tactggcggc ggtgggcagg aaggtggttt caaagaggtc ggttgtcggg tcaggagcct
      901 cagcccaaac ggccacttcc gtctccccgt cttgccggct gagcaacagg ccgcgcaccg
      961 gccggccgtc ggcggccgta tagatgaagt cgttgcgctc ccagatgtgg ccggcgtaaa
     1021 agaagcgctc cggctcgtcg gcggcaaagc cggcaacgcc ctcggaggcg gtggccagca
     1081 ggctgctcat cgcctcggcc gctgttcggg ctgccggctg gatgcgcacg gcaacgaacg
     1141 tctgcgcgtc ggcggcgaag cgatgccagt tgttgtatgg ctcataggcg aagccggcgg
     1201 ggtagtgcag gcgaaactcg cccacgttga gcgttgacca gggttcgccg ccaaagccca
     1261 gccgcggtgg caggcgctgc cacgtcgcgg cgatcgtgcc caacgtagcc acagtcgtcg
     1321 cctcggcctc gcgcgggcca tccaggtcga tgacagtgcc cgtgccctcc tgcacgaagg
     1381 tgacaaacgc gcccgtgcgc ggcccctgct cggcgctgtc gtagccataa gccgtgcgta
     1441 ccccctcgac gcccggcgcg gggccgaccg tcaccggctc ttgcaacagg acgctgacct
     1501 ggccgaaggt gcccagaacg tcattttgca gcgtggccag gtcgccggcc cagttggggt
     1561 aggtgcgcac ctggagttgg gccgtgccgc tgacgtcgcg cgtgaaggtg atgccctcct
     1621 gcgtcgccgg cggcagccag ccggccggat agaggaattg catgctgttg gtcacatcca
     1681 cgtaggcgcg gaagccctcc ggcgcgccgg tcggatcgac cgtcactggc tgcgtggcca
     1741 gcgtggcgct gctgtcgagc gcggtcacac gcaggccaac ggcgtagcgc ccggcgggca
     1801 gcggccgggc gctgcggtag agcgctggcg cgtcgtcgta gaccagcatc gcgccaggct
     1861 cggtgctcag cgtgccgtcg gcggccagga ccagaagcga gggctggaag gtgtcgcctg
     1921 cttgcggccg cgcctcgccg atcaaccgcg cgccggcgtc ggcggggaag gtcagccaca
     1981 ggcgcgatga cgccggcgcg ccgggggcaa agacgaccgc agcctcggcg gcgcgatcgc
     2041 tcccggcgcg gcggaagaag ccccgcgcgg cggtctgcgg ccccaacggc gagggggcca
     2101 cggctcgcag cggcgtatag tcgcccgcgc cggcgctgtc ggacagatag ctgccagtcg
     2161 cgtcccagac gacaagtgat tcgtgccagc cgtcgctcca gagagacgtg tttgtgccgc
     2221 cgggcaaggt ctgcgcggtc ggcgcgacaa cttcctcctg gcgcagcaca tagcggccgt
     2281 cggctgcctc ctggcccgcc accagcgcga cctggcccac ccgttgggcg acgatctcgg
     2341 cgcgcaggaa ggccggttgg gcaatatggg ccacgggcgg ggctggcgtt cgcgggtccg
     2401 gctcgacggt cagccgcgtc gtgctgccgg ccgtcgcccc gacgaaggcg ttgagatagc
     2461 cggcccaggt gggcaggcga ctgatgccgc cgtagagagg atcgaccgcg gccacactgc
     2521 ccggccagta gagggcgacg ccacgcccca tcgccagccc ctggccgtgg tcaaaggcga
     2581 ccagcgcttc cgtcagggcg gcgctgacgg ccgtggccgc cgtctgcacg tcgccgggcg
     2641 ggctgacctc ggcgatgagg gctgcggcgt gggacagatc gacggcggcg atgctgtcgg
     2701 cgtcggccag ggcggcgcgg ccgtagacct gcgcgccgcg gcgcgcaaag ccggccagct
     2761 cgccgtagag ccgcggctcg gccgacagcg cgccggccag cgcctcgacc gcggcgctga
     2821 gggccgggat atgcgccaga tcgaccgcgg ccatgctggc gaattcatcc ccctccagct
     2881 ggcgctgcgt gttgacgtaa gccgtaacca tgtcgccggc cagttgccgc ccgtcgacga
     2941 gcggctcgct gttgagttgg gccacaaccg cctgaaagtc ccacgacgcg ccggccacca
     3001 ggtcgggcga ggccacggcg taggcagcgt agggctggat ggtctgtagc acgtctagtt
     3061 ggctcatcag cccggcagcg aaggcgacga cctcgaagcg cgtgccggtc tgtgcctgcg
     3121 cgtaggccag ggcctggtcg atgtcggtca gactgaggtg gtctgtctga tcgggcgcgc
     3181 cggcggtcgt gtccaggcag cagccccgcc agccgccgcc gtagtcgccc agtaccagca
     3241 gggtgcgatt ggccgggtag gcgctgaccg cccacgtcag gaagtcggcc aacgtctgcg
     3301 ggtcgccctg gtttgtctcg cccagagtgg cgaccacttc ggaggccagc gcctgcgggt
     3361 cggcgtcggc gcgaatgtgg tagcgggcgg actcggctgt gcccgccggg ccgccctccg
     3421 gccaatcgac ctgggccacg acctgcacct ggtctgatgg cgcgccggct tccatctcgt
     3481 tgaggtcgtc ccagacgaag gcggcacggt cgttgtcggc gccaccgtag tagagcaccg
     3541 tccaggcggg gcgggcggga tcgtaggtgg ccgtaggccc cgcggggggg ggcaggacgg
     3601 cagttgtggc cacgctgtcc ggcggcgcgg atgcgccgtc gggcgcttcg gctgtgacac
     3661 cgaggacaac gggcgacgcc acaggcggct gcggcgtcag ttgcgccagc cgtccgccgg
     3721 ggtcgcaggc gaccagagtg aggctcagcg cgagcagtcc cagagagatg cagaggcagc
     3781 gaatcggccg agaacgtatc attcggcatc attatcgact gagttgggcc gggaggcaaa
     3841 atcagagcgt caccggaggg agaacgaaga tggggattga cttgagactt gaactgaaaa
     3901 accctgatct ctgacggtct tgacttgtgc agccgcggca aacacgttag catttgggtg
     3961 tctgcccatc cacagcgagc gcctgccatg cctgacgact ggtacagcta cagcggtaat
     4021 ctgcacatcc acacgcgtta ctctgacggc gaaaagctac acgccgagat cgcccacgat
     4081 gccatcgccg ccgggttgga cttcatcatc atcaccgacc acaacgtcta cgtgaccggc
     4141 gtcgagggtt attaccacaa cgacaaaggg cgcgtcttgc tcatgactgg cgaggaagtc
     4201 cacaacgtgc gccgccagcc gcaggccagc cacttcctgg cctatggggc gggcagggaa
     4261 ttagccccct tggccgctga tccgcaagcg ctgatcgacg agacgctcag cgccggcggc
     4321 tacggcttcc tggcccatcc gcacgaaaaa gacctgcgta tcatcgactc gcctgacctc
     4381 ggctggcacg actgggacat cgagggcttc agtggcctgg agatttggaa ctacatgtcg
     4441 gtcttcaaga acactgtcgc tgcccggctg gaagggctgc gctggcgcaa caaggcgact
     4501 gagtcgctgc tggccctgtg gatgacgctg cacccagagc gctacatgac cggggccgac
     4561 ccggagacgc tggcgctgtg ggacagcttt ctggctaagg gcatgcgcat caccgccgtg
     4621 ggcaatagcg acgcccacgg cacgccaatg cacctcggcc ccatccaccg cgaggtctac
     4681 ccctacgagt tccttttccg ggcggtcaat actcacgtac tgacgccgga accgctcaac
     4741 ggcgacttcg accacgaccg ggcgctgtta ttgcgggcca tcggccgcgg ccagagctgg
     4801 gtgggctacg acatgcccgc gtccacggct ggctttcgct acacggtcaa aggcacccgc
     4861 aggggcatta tcggcgagga gatgccgctc ggcgcggctg gagcgacctt gcaggtgaag
     4921 acgccggcgc gctgccgcat ccgcatcatt cgccacggca gtgttgtggc cgaggccgag
     4981 cgcgacacca acctggccta cgcgccgtca gaaccggggg cctaccgcgt ggagggccac
     5041 attctatatg agggacagga gcgaggctgg atctacagta atccgatcta cttgcgctaa
     5101 cgcgccgccc gccgccgtgc ctggcacttt ccgaagtgcc tggcacgttt ttgaatgccc
     5161 taagaacgtg cgacgccccc ggcggggatc attccgtcgc cgccaggacc gcgcggccaa
     5221 ttgcttcggc cgcggcgatg tcgtcttcat tctctagcac cagggcaata acgaagcgcg
     5281 gcgcgtcggc cggggcaaag cccaagtacc aactgttgtg gtggccatcc gggccagaga
     5341 gaggcgacac agcgaaccct cgtgaactcg ccgcaagctc gccggcgtcg ccgctgcggg
     5401 gccaggtctg gcgcaccgcg gcggccgtgg ccgccctgac agccgttgtc gcgacaagag
     5461 ccgacggctg accgacccat tggccgctcg cgtcggtgac ggcctccacc agtcgcagca
     5521 cgggccgccg gccgccggcg gccagggtgg tcgtggccag cgccgcttgc aggggtgtca
     5581 ccgtgagccc cgcctgcccg atggccgcca gggccggatc gtcgatgtcc gccgggctgc
     5641 cactcgtggc aatcggcagt gccggcgccg tcgtcaggcc aaagacgtcg aagacctcgg
     5701 ccagcgccgc cgcgcccatg tcgcgtccca gcgcttgcag cggcgcaggg cagcgcaggg
     5761 cagccatgtc ggcccaggtg tagcgcgccg ttgggccggt ctcttcctgg cgttggacgc
     5821 agcgctgcac ccggccgtcc agggtgactg gcgcaaaggg gtcggcgaca atatcgccca
     5881 gttggatgcg cgcggcatcg accgccgccg cggtcagaaa gggctggacg acaaggccgg
     5941 gctggtacag gccctggctg gcccggtcga acaacggccc gcgcacgtcg gcggccaacg
     6001 tctcgaactg ctccgccagg gcgttgggat cataatccgg ctcgctgacg agcgcgcgga
     6061 tggccgctgt gggcgtttct gcgtccggct ctagctccaa cagaaccagg ccgccgacat
     6121 gcccggccag ctcggccgca gccgtccgtt gccactccgc gtccaacgtc agccgaacgt
     6181 cgcgaccctg gtgggagacg tgcagcaccg cctgccagac ctcgtcccag agcgcgccac
     6241 tgtggccgcg cagggccgcg tcgtaggcgc gctcgatgcc cgccgcaccg aagcgcacgc
     6301 tgtagtagcc cacggccggg ccgctctcag acagcgggta gacgcgcgtc agcgcgcccg
     6361 tctcgccaac tgactcggcc aacacaacgc cgttccggtc gacgatccgg ccgcgttgga
     6421 cgcgccgctc ggcctccacc agccgcgggt tgtccgggcg ggcggccagg acttcgctgc
     6481 gcacggcggc ccagtaggcc agcgtcaggg cgacaacgcc aaagccgagc agcaaggcca
     6541 gccccagccg ctcgatacgc cgggcgagac gcgggtcgta ggcgcgcgtc ggcgttgccc
     6601 catcgttggc cgcggacagg tagagcagca ggccgatcat gatgctactg atcaggagcg
     6661 aactgccgcc gtagctgatg aacggtaggg tgatgcccgt cagcggcagc agcttgatga
     6721 cgccgccgat gatgagcatc gcctggatgg cgaacaggac gaccagcccc gcggccaggt
     6781 aggcgtggaa ggtgcgcggc cggaagccct gcaagctcag cagcgccgcg cgcaggccgc
     6841 gaaaggtgac cagagcgatg gcgaagacga cagtcaggct gccgaccagg ccccactcct
     6901 cggcaatagc tgccagggca aagtcggaat ggacgacggg gatgtagtcg ggaaagcctt
     6961 gccctgcgcc gcggccgaaa atgcccccgg cggcctgggc gtagagcgac tggacgacct
     7021 gataggcgcg gtcgctgaca ttgggccagg gattaagcca ggtcagaacg cgcggcgcga
     7081 ccgcgttgaa ggcgaagaag gcgaagacgg ccgcggccat cagcaaaaca atgccactca
     7141 cgacgtaaag ccgttcgccc gtagccagat aaagcaaggc gacaaagacg atgaaaaaca
     7201 gtcccgccgc ccccaggtct tgctgccaga ccagtagcag caggcaaaag ccccacatga
     7261 gcaccagcgg ccccaagaag gggagttggt ggagcaggcg gcgggccggg ttggggcgcg
     7321 ccccgttgcc cgccgcgcgc tgccggttgt agcggtagag cggctcctgc tcggtgaagt
     7381 agctggccag aaagatgacc agcagtagct ttagcaattc cgacggctgg aagtagacta
     7441 cgtccaaaaa cggcacgggg atcgagagcc acagcgccgc gccgccgccg ctgggattga
     7501 cgccaaagac aagtgtgacg gccaccaaca gtagcccacc caccagcagc acgtagcggt
     7561 agcgcgtcag cggctggagc gagcgcggga cgatggcgac gagaagcagc gccgccgtgg
     7621 ccaccacgta ccaaagcgtc tgccggccca ggaagttggg cgccaggcgg tcttgcaaca
     7681 gcaggcccca gccggccagc agggcataaa ccggcagcag gaagggatcg cggtggggct
     7741 tcaggtggcg caaggtcatg tgggcggcga cactgaccaa cagccacacc gccggccaca
     7801 ccagccgcgg ccacgaccag tcctgcatcg tcagggccag cgtggcggca ttggccacga
     7861 cgaacaacgc cgccaacgcc agcaacagtc cttcgcgccg gtcgcgcggg cggtcgcgct
     7921 cttccaggcg ctggcctgcc cattgtcgcc aggcgaccgg gccggagcgg aaactcgtca
     7981 tccgggcaaa tacttgccga tgagcggttg caggttggcg cgcacgtcgg gggagatatg
     8041 ctcggccggg gtaatgatgg cgtccttcag ggcgctatgg gccgggaact cgcccgccca
     8101 gtcgtcatag ccggccacga gggcgctgat ggccgcctgg gccagctgcg tattggcccg
     8161 cagcacgcgc acgaccatct cgaccgtaac cggggcctcg ctgtggtgcc agacgtcgta
     8221 gtcagtgatg tgaaccatgc aggcgtagct aatttcggcc tcggcggcca ggaacgcctc
     8281 cgggctggta gtcataccga tggcgctcat gccccactgg cggaaaatct ccgactcgcc
     8341 acgggtgctg aagcgcggcc cttcgacggt aatgatgttg ccgccgcgat ggagccggcc
     8401 gccagccgct tccgttgcct cggccagcgc cgcgctcaga tgggggcaaa atggctgagc
     8461 cacggtcacg tgggcgacca ggccattgcc aaagaacgac cgctcgcgcg cgcccttggt
     8521 gaagtcgaat agctgatcgg gcacaacg
//
LOCUS       DAOSMQ010000072         8368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8447_length_8368_cov_4.651871, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000072 DAOSMQ010000000
VERSION     DAOSMQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8447_length_8368_cov_4.651871"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(500..3265)
                     /locus_tag="PK829_03780"
     CDS             complement(500..3265)
                     /locus_tag="PK829_03780"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HOU40370.1"
                     /translation="MATLQQLHDLGQSTWLNYLRGSFIHSGELSERLKQGIQGVTANA
                     QVYERAIRGSSDYDTGIRREMAAGKPARHVHEALMVDDIQRAADALHPIFETSHRLDG
                     FVSYEMDPSTLQSVPAAVAEVRHLSHHIDRYNVMVEIPATPIGIAAVRDLVADGVSIN
                     VTHLFSLADYEAVAAGYLAGLEQYITSHSVWRTAPTAVASFSLAPIDAVVDAQLEALG
                     RPDLSSKTAVALARRLYARYQQLFSGPAWERLAARRGQPLRPKWTRTTPRDFRHAPLY
                     YVEQLIGPGTVMTFSPATLNVFLREGIAAVALDKGLDAAETHLATIAALGIDLEAVAE
                     ELKAEHLAASERRFQTLVEAVAAKRNALDMGRPTMALQLGEAQAAVDEGLHDLCRHHI
                     IRRIWAADHTVWNPQPKEITNRLGWLDIADNMADKTARLEAFTQSLVAGGFRSALLLG
                     MGGSSLAPELFARTLGRAARPAGAPYPYLDLVVLDTTDADAVRAAEARVDLAHTLVIV
                     ATKSGGTVETLSAFKYFYNRLLAVVGPERVGGHFIGITDPGSKIADMAAQYGFRDLFL
                     NDANIGGRYSALSYFGLVPAALVGVDVPTLLERAAIAAANATLSHCDAIGDNQAAVLG
                     ATLGKLALAGRDKVTFITPPALASFGDWVEQLIAESTGKEGQGILPVVGEPRAPIESY
                     GPDRLFVHLRLEGDASTDAFAADLAAAGHPVVTLRLGDLYDIGGQFFLWEMATAIAGY
                     FLGINPFDQPNVEAAKVKAREIVAAFATTGSLPAGDFVAPEPGALAGFLESARPGDYV
                     AIQAYIQPSPAADEALRALRQAIHLRTGLATTAGYGPRFLHSTGQLHKGDAGHGLFIQ
                     IVSEAVEDVGIPDEAGQAAAAMSFNVLKKAQALGDARALRDGQRRVISFAVGTEAVAA
                     IERLAAL"
     gene            3742..4353
                     /locus_tag="PK829_03785"
     CDS             3742..4353
                     /locus_tag="PK829_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU40371.1"
                     /translation="MDDHEAKEPPLAMLSGAARGPMTDTTSDTSLLARTARGDAAAFE
                     ALFQRHYDRVYGILFRLVGERGEAEDVAQEVFLRLHDHARRQRPDDNVTAWLYRVATN
                     LGYNALRSRRRRAQRNMVLVPSPAGGDVDTVLARREERRAVRAALSRLTEREAQLLIL
                     RQMEFSYAECAAIVGVAPGSVGALLARAGRAFRAAYERERGGE"
     gene            4543..5370
                     /locus_tag="PK829_03790"
     CDS             4543..5370
                     /locus_tag="PK829_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40372.1"
                     /translation="MSTRRVVSVGLTLVVALAVLMAFPSVRAAASDFLGLFRVQQFAP
                     ISVSPQQLALLEQLGEEGLAPGEFVVTQELGEPETVDSPKVAAAVAGFMPKTLPNRGA
                     LIRTYVMTGGAGYLTVDLAGARAIVEAAGADPLLLPDSLDGARVDVTVYDSVGQLYDN
                     GVLLLQSPSPDVNYPTDVDPTVLGEALLQVLGTEPSEAHRIAQSIDWTSTLLLPVPQE
                     LGTYREVTVNGVGGVLLEPFDSKAEKAVVWQQDGMVYMMRAGQLSVDELLAQANSVR"
     gene            5499..6422
                     /locus_tag="PK829_03795"
     CDS             5499..6422
                     /locus_tag="PK829_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40373.1"
                     /translation="MTDLVIDTRGLRKEYGAKVAVADLTLDVRPGEVFGFLGPNGAGK
                     TTSIKMLLSLTKPTAGEATLLNRPLGDLAARTRIGYLPEHFRFHEWLRADEFLNLHGE
                     LYGMDRAARGVAVPRLLALVGLEDRAATKLGAFSKGMLQRIGLAQALLNDPALVFLDE
                     PTSGLDPIGRRVVRDIITDLRERGMAVFLNSHFLSEVEKTCDRVAFIRSGRILQTSSL
                     KEFEQEAMQVVVRVGQPTPELLAGLARFGQNVVQQPDDGRIHLTLGDEAQLPALANWV
                     VQSGYSLYELTPAHLSLEERFLQVVGEEMDE"
     gene            6425..7258
                     /locus_tag="PK829_03800"
     CDS             6425..7258
                     /locus_tag="PK829_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU40374.1"
                     /translation="MAILVIARLTIREAQRRRLLWVALFMGVAFLLVFGLAFHFIRLD
                     IERHAAATDQLAFVSVFLLTAGLYAVNLLVNVVAVLVSVTTISGEIESHTIDAIVTKP
                     IPRWQVVVGKWLGYAALVLLYLVFLVGGLMLIVYLRSGFMMDNIGRGVLMMALAALLI
                     LTMSIAGGTRLSTIANGVLTFMLFGIAFLGGLVEQVGALLRNQAAVNVGIVSSLIMPA
                     DALWKKAVAYFQTNSPTTPFELGPFATITEPSTLMVVYAVIYLVGLLLFALWSFSRRD
                     L"
     gene            7344..7937
                     /locus_tag="PK829_03805"
     CDS             7344..7937
                     /locus_tag="PK829_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40375.1"
                     /translation="MAEKPHIIRIVADQVRRQYYRRQCSRAELPRPRIQGDEIGVELE
                     DGRFIPYADLGYHDEVGFFRLSETAGVPNQAHPYAASGRLIDCGQVVVTTSASLRLTP
                     DEIGTLIERHASGDFGENGEFYELDVSDEMLHDPGAQFLPIGIINKINTLTGLDPICS
                     AYTVREHAIWVITEAGEQRTTLVLYAGAADASASSAR"
     gene            complement(7895..>8368)
                     /locus_tag="PK829_03810"
     CDS             complement(7895..>8368)
                     /locus_tag="PK829_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU40376.1"
                     /translation="MFGGYIGAGTKTVLPSTGHAKISMRLVPDQDPAEIAALFEKFVR
                     ETLPATVRAEVKVAGLSQPVLMARDTPEMRAAAAAYEATYGQPAVFMREGGSIPVVSD
                     LQNVLGLPTVLMGFGLPGDRIHSPNERFYLPNFYAGIETSIRYLALLAEASAAPA"
BASE COUNT         1350 a         2732 c         2804 g         1482 t
ORIGIN      
        1 actgacaccg gtcgtggata tgggtgggta gagtatgaag gaacgcgatt gaacctttac
       61 tcaatggcct ggtggggcct ccaacctgga gacaactacg aatggtcagt aacggtcagg
      121 aacgattatg cgtggagtga tgaatcggct ccttggtcat tcagcattct gccaccaaca
      181 aatggccgac agcccagtac tccgtcatct tcgttaactc tccagcgcat gaaggccggt
      241 acgacctgga tctatagtca ggactaacta cttccactcg gaacaataca ttacccccca
      301 ggtttcttcc gagaaacccg gggggtaatg ccaccttctc aatcacccgc cacggcaaca
      361 acggtatctc atccatccac acccccgtcg cgtcgtggaa tgcggcgact ccgccccgtc
      421 cttccccgac cgcaaccgct gacggccgcc cggcccgccc ccacctgctc tcgccgcaat
      481 ggcgggttag ccgccaacgc tacaacgccg ccaatcgttc aatcgccgcc accgcctccg
      541 ttcccacggc gaagctgatc acccggcgtt ggccgtcgcg caacgcgcgg gcgtcgccca
      601 gggcctgcgc cttcttcagg acgttgaagc tcatggccgc ggccgcctgc cctgcctcgt
      661 cggggatgcc cacgtcttcc accgcctcgg acacgatctg gatgaatagc ccgtggccgg
      721 cgtcgccctt gtgcagttgg ccggtggagt gcaggaagcg cggcccatag ccggccgtcg
      781 tcgccaaccc ggtgcgcagg tggatggcct ggcgcagggc gcgcagggcc tcgtcggccg
      841 ccgggctggg ctggatgtaa gcctggatgg cgacatagtc gcccggccgc gcgctctcca
      901 ggaagccggc cagcgcgccc ggctccgggg cgacgaagtc gccggccggc aggctgcccg
      961 tcgtggcgaa cgcggccacg atctcgcgcg cctttacctt ggctgcctca acgttgggct
     1021 ggtcgaacgg gttgatgccc aggaaatagc cggcgatggc cgtggccatt tcccacagga
     1081 agaactggcc gccgatgtcg tagaggtcgc ccagccgcag cgtcacgacc gggtggccgg
     1141 ccgcggccag atcggcggcg aaagcgtcgg tagaggcgtc gccctccagg cgcaggtgga
     1201 cgaacagccg gtcggggccg tagctctcga tgggcgcgcg cggctcgccg acgacgggca
     1261 ggatgccctg gccctccttg ccggtgctct cggcgatgag ctgctcgacc caatcgccga
     1321 agctcgccag cgccggcggg gtgatgaacg tcaccttgtc gcggccggcc agggccagct
     1381 tgcccagcgt ggcccccagg acggcggcct ggttatcgcc gatggcgtcg cagtggctca
     1441 gcgtggcgtt tgccgcggcg atggcggcgc gctccagcag ggtgggcacg tcgacgccga
     1501 ccagcgccgc ggggacaagg ccgaagtatg acagcgccga gtagcgcccg ccgatgttgg
     1561 cgtcattgag gaagaggtcg cggaagccat attgcgcggc catgtcggcg atcttgctgc
     1621 cggggtcagt gatgccgatg aagtggcccc cgacccgctc cggcccgacg acggccagca
     1681 gccggttgta gaagtacttg aaggccgaca gcgtctcgac cgtgccgccc gacttggtgg
     1741 cgacgatgac cagcgtgtgg gccaggtcga cgcgggcctc ggcggcccgc accgcgtcgg
     1801 cgtcggttgt gtccaggacg acgagatcga ggtagggata gggcgcgccc gccggccgcg
     1861 ccgcccggcc gagcgtcctg gcgaacagct ccggggccag gctcgagccg cccatgccca
     1921 gcagcagcgc cgaacggaag ccgccggcca ccaggctctg cgtgaacgcc tccaaccgcg
     1981 ccgtcttgtc ggccatgttg tcggcgatat cgagccagcc caggcggttg gtgatctcct
     2041 ttggctgtgg gttccagacg gtgtggtcgg cggcccagat gcggcggatg atgtgatggc
     2101 ggcagaggtc gtgcaggccc tcgtcgacgg cggcctgcgc ctcgcccagt tgcagcgcca
     2161 tcgtcggccg gcccatgtcc agagcgttgc gcttggcggc cacggcctcg accagggtct
     2221 ggaagcggcg ctcggaggcg gccaggtgct cggccttcag ttcctcggcc acggcctcca
     2281 ggtcgatgcc cagggcggcg atggtggcca ggtgcgtctc ggccgcgtcc agccccttgt
     2341 ccagcgccac cgcggcgatg ccctcgcgca ggaagacgtt gagcgtcgcc ggcgaaaagg
     2401 tcatcaccgt gccggggccg atgagttgct cgacataata cagcggcgcg tggcggaagt
     2461 cgcgcggcgt ggtgcgcgtc cacttgggcc gcagcggttg gccgcgccgc gccgccagcc
     2521 gctcccaggc cgggccgctg aagagttgct ggtagcgagc atagagccgc ctggccaggg
     2581 cgacggccgt cttgctggac aggtcgggcc ggccgagcgc ctccagttgg gcatcaacga
     2641 cggcgtcgat gggggccagc gagaaggagg ccacggccgt tggcgctgtc cgccagacgc
     2701 tgtggctggt gatgtactgc tccagcccgg ccaggtagcc cgcggcgacg gcctcatagt
     2761 cggccagcga gaacaggtgc gtgacgttga tcgacacgcc gtcggccacc aggtcgcgca
     2821 cggcggcgat gccgataggc gtggccggga tttcgaccat gacgttatag cggtcgatgt
     2881 ggtgcgacag gtggcgcact tcggccacgg ccgcgggcac gctttggagc gtcgatggat
     2941 ccatctcata gctgacgaag ccatcgaggc ggtggctggt ttcgaagatg gggtgcagcg
     3001 catcggcggc gcgctggatg tcatcgacca tcagcgcctc gtggacgtgg cgggccggtt
     3061 tgcccgcggc catctcgcgc cggatgccgg tgtcgtagtc gctgctgccg cgaatggcgc
     3121 gctcatagac ctgggcgtta gcggtgacgc cctggatgcc ctgcttcagc cgctccgaca
     3181 gttcgccgga gtggatgaag ctgccgcgca gatagttcag ccaggtggac tggccgaggt
     3241 catgaagttg ttgcagggtt gccatggcga ctcctttggg ggacgtggct gcttgtgggc
     3301 tggcgaaggt tcgtaaggat ggtggcccgt gggggccatg cgcccgatcc gcccgggccg
     3361 gccggggctg cccggggatc gtctctctat agcatagcgc gatcgcggcg catcttcaag
     3421 cgggaacgtg gggtcatcac ggcttttcag gtagtagtac agcctgtcag gctgtcatcg
     3481 ctgggcacag ccacagccta acaggctgtg ctacaggctg tgctacgtgt gcgtcgagat
     3541 gcgcggcgcg aagaccgact actgaagccc tggtagggtc gctgcaaagt cggaagaagc
     3601 gctcacgtga agatctccaa gggggcaaag ggcgccaaaa agaggctata ctgcatctgg
     3661 gggccgccgg gcgtcctcca cgcaaagcag cccaatccgg ccgctgcctg tcacaaacgg
     3721 gcgcgcggcg gtgtttagaa gatggatgat cacgaagcaa aggagccgcc attggccatg
     3781 ctgagcggcg cggcccgcgg ccccatgacc gacaccacca gcgacaccag cctgctggcg
     3841 cgcaccgccc gaggcgacgc cgccgcgttc gaggcgctgt tccagcgcca ctatgatcgc
     3901 gtctacggca tcctgttccg gttggtgggc gagcgcggcg aggctgaaga cgtggcccag
     3961 gaggtcttcc tgcggttgca cgaccacgcc cgccgccagc ggccagatga caacgtcact
     4021 gcctggcttt accgcgtggc cactaacctg ggctacaacg cgctgcgcag ccgccgccgc
     4081 cgcgcccagc gcaacatggt gcttgtgccg tcgccggccg gcggcgacgt cgacaccgtg
     4141 ctggcccggc gcgaagagcg gcgcgccgtc cgcgcggcgc tgtcgcggct gacggagcgc
     4201 gaggcgcagc tgctgattct gcgccagatg gagtttagct atgccgagtg cgccgccatc
     4261 gtcggcgtcg cgcccggctc tgtgggggcg ttgctggccc gcgccggccg tgcctttcgc
     4321 gccgcctacg agcgcgagcg gggcggcgaa tgaccgccaa tggcggaggt cgagatgaat
     4381 gagcaggagc atgaaatccg cgagacgcta gagcgcctgg ctgtgctggc ccccgaccct
     4441 tcagagtcgg ccgcgccggc tgacgcgctg gcccgttttc gtcaccataa cgccgcgcgg
     4501 cctcgcgccg ccggcctggc aagtataagg agacaaacaa ttatgtcaac ccgtcgtgtt
     4561 gtgagtgttg gcctgaccct ggttgtggcc ctggccgtgc tgatggcctt tccgtccgtg
     4621 cgcgctgccg ccagtgactt cctgggcctg ttccgcgtgc agcagttcgc gcccatctcc
     4681 gtctcgccgc aacagctggc gctgctggag caactgggcg aggaagggct tgcgccgggt
     4741 gagtttgtcg tcacccagga gttgggcgag ccggagacgg tcgattcgcc caaagtagcg
     4801 gcggccgttg ccggtttcat gcccaagacc ctccccaacc gtggggcgct gattcggact
     4861 tacgtgatga cgggcggcgc gggctacctc acggttgacc tggctggcgc gcgcgccatt
     4921 gtcgaggcgg ccggggctga cccgctgctg ctgcccgaca gcctcgacgg ggcgcgggtg
     4981 gacgtgaccg tctacgatag cgtggggcag ctctacgaca atggcgtcct cctgctccag
     5041 tcgcccagcc cggacgtgaa ctatcccact gatgttgacc cgactgtgct gggcgaggcg
     5101 ctgctccaag tgctgggcac ggagccgtct gaggcccacc gcatcgccca aagcatcgac
     5161 tggacgagta ccctgctctt gcccgtcccg caggaactgg gtacctaccg cgaagtgacc
     5221 gtcaacggcg ttggcggcgt actcctggag ccgttcgatt ccaaggcgga gaaagctgtc
     5281 gtctggcagc aggatggcat ggtctacatg atgcgggccg gccagttgag cgttgacgaa
     5341 ttgttggcgc aggcgaactc ggtgcggtaa ggtacatccc tcaaagattc atctattccg
     5401 tccccagatt gagccgttca ggcgcgtgat ccggagcgag ttcgccttgc tccggatcgc
     5461 cgtccaaact gcccgatctg cggcttcatc ctatcaaaat gaccgatctt gtcatcgaca
     5521 cgcgcggcct gcgcaaagag tatggcgcca aagtggccgt cgccgacctg acgttggacg
     5581 tgcgccccgg cgaagtgttc ggcttcctcg gccccaacgg cgcgggcaag accacctcga
     5641 tcaagatgct cctcagcttg accaagccca cggccggcga ggcgaccttg ctcaaccgcc
     5701 cgctgggtga cctggccgcg cggaccagga tcggttatct gccggaacac ttccgctttc
     5761 acgaatggct gcgcgccgat gaattcctca acctgcacgg ggagctttac ggcatggatc
     5821 gcgcggcgcg gggcgtggct gtcccgcgcc tgctcgcgct ggttgggttg gaggatcgcg
     5881 cggcgacgaa gctcggcgcg ttttctaagg ggatgctgca acgcatcggc ctggcccagg
     5941 cgctgctgaa cgatcccgcg ctcgtttttc tggacgaacc cacctctggc ctggacccca
     6001 tcggccgccg cgtggtgcgc gacatcatca ccgatctgcg cgagcggggc atggccgtgt
     6061 tcctgaactc ccacttcctg agcgaagtgg aaaagacgtg cgaccgcgtc gccttcattc
     6121 gcagcggccg catcttgcag acatcctcgc tcaaggagtt cgagcaggaa gccatgcagg
     6181 tggtggtgcg cgttggccag ccgacgccgg agttgctcgc ggggctggcg cgctttggcc
     6241 agaacgtcgt ccagcagccg gacgacggcc gcattcacct gacgctgggc gacgaggccc
     6301 agctgcccgc gctggccaac tgggtggtgc aaagcggcta cagcctctat gaattgacgc
     6361 cggcccacct gtcgctggag gagcgcttcc tgcaggtggt cggcgaggag atggacgagt
     6421 aaccatggcg attctcgtca ttgcccggct gactatccgc gaggcccaac ggcgacgcct
     6481 gttgtgggtg gcgctgttta tgggcgtcgc ctttctgctg gtctttggcc tggcctttca
     6541 cttcatccgg cttgacattg agcgccacgc ggccgccacc gaccaactcg ctttcgtctc
     6601 tgtctttctc ctgacggccg gtttgtacgc ggtcaacctg ttggtcaatg tcgtcgccgt
     6661 cctggtctcc gtaacgacca tctccggcga gatcgagtcg cacaccatcg atgccatcgt
     6721 gaccaagccc attccgcgct ggcaagtggt ggtgggtaaa tggctgggtt atgcggcgct
     6781 ggtgctgctc tacctggtct ttcttgtcgg cgggctgatg ctcattgtct atctgcgctc
     6841 cggcttcatg atggacaaca tcggccgtgg cgtgctgatg atggctctgg ccgcgttgct
     6901 tatcctgacc atgagtatcg cgggcggtac gcgcctttcg acaatcgcca atggtgtgtt
     6961 gacattcatg cttttcggca tcgccttcct gggcggtctg gtggagcagg tgggcgcgct
     7021 gctgcgcaac caggccgccg tcaacgtcgg catcgtcagc agcctgatca tgccggccga
     7081 cgcgctctgg aagaaggccg tcgcctactt ccagaccaac agcccgacca cccctttcga
     7141 gttagggcct ttcgcgacca tcacggagcc gagcacgctg atggtggtct acgccgttat
     7201 ctaccttgtc ggcctgctgt tgtttgccct gtggagtttc tcgcgccgcg atctgtgaaa
     7261 agcgcgggta aatctctcct ttcgcctgtg atttcgccta taatatgggc aagtatcccg
     7321 accgtagcgg gagaacgcgc acgatggcag aaaaacccca tatcatccgc attgtggccg
     7381 accaggtccg ccgccaatac taccgccgtc agtgctcacg cgccgaactg cccaggccgc
     7441 gcatccaggg cgacgagatc ggcgtcgaac ttgaggacgg ccgcttcatc ccctatgccg
     7501 atctgggtta tcacgacgaa gtgggcttct tccgcctgtc ggagacagcc ggcgtgccca
     7561 accaggccca tccctacgcc gcctctggcc gtctcattga ttgtggtcag gtcgtcgtca
     7621 ccaccagcgc ctccttgcgg ctgacccctg acgagatcgg cacgctcatc gagcgtcacg
     7681 ccagcggcga cttcggcgag aacggcgagt tctacgaatt ggacgtcagc gatgagatgc
     7741 tgcacgaccc tggcgcacag ttcctgccta taggcatcat caacaagatc aatactctga
     7801 ccggtctcga ccccatctgc tcggcctaca ccgtgcgcga gcacgctatc tgggtgatca
     7861 ccgaagcggg cgagcaacgg acgacgctgg tgctctacgc cggcgcggcc gatgcctcgg
     7921 ccagtagcgc caggtagcga atgctggtct cgatacctgc gtagaagttg ggcaggtaga
     7981 agcgctcatt gggcgagtgg atgcggtcgc cgggcaggcc aaagcccatc aacaccgtcg
     8041 gcaaccccag cacattctgc agatcgctga ccactggaat cgagccgccc tcgcgcatga
     8101 agacggccgg ctggccatag gtcgcctcgt aagctgcggc cgcggcgcgc atctccggcg
     8161 tgtcgcgagc catcagcacc ggttgggaga ggccggcgac cttcacctcg gcgcggacag
     8221 tggccggcag cgtctcgcgg acaaacttct caaacagcgc ggcgatctcg gccgggtctt
     8281 ggtcgggcac aagacgcatg gaaatcttgg cgtggccagt ggacggcagg acagtctttg
     8341 tgcccgcgcc aatgtagccg ccaaacat
//
LOCUS       DAOSMQ010000073         8358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8462_length_8358_cov_4.214862, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000073 DAOSMQ010000000
VERSION     DAOSMQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8462_length_8358_cov_4.214862"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="PK829_03815"
     CDS             <1..1856
                     /locus_tag="PK829_03815"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HOU40377.1"
                     /translation="LFVAVLEMATALRRAAEAAGTATHVAPLPPEQAAAATRLAEQRE
                     AAMRQSIRAAAAAGRDRIAAVCGTWHAPALTAALDTPPDADAALLRDLPAIEVAATWV
                     PWTYSRLTQAGGYGAGVASPGWYDHLWAMGEAGGAFQRNVAAVWLANAARLLRGEGFD
                     TSPGHLIEAVRLAEALAALRERPFPALEELGEAAQSVLCGGDAEPMALIRQRLVVGER
                     MGIVPPDVPAVPLQRDLRAQQTRLKLPPEPEPVALSLDLRQEIDLERSRLLHRLALLD
                     IPWGETATTKGQVGTFAERWQLQWRPELSLRVIAAAMWGNTVRDAAAAHTGDLAARTD
                     ALPALIALIDRAVLADLPEAIPAVLTRIEELSAGSADVVHMLAALPPLAQVLRYGGLR
                     HTAEHLPLLRRVFDHLLTRACLSLPRATVAIDDEAARDLAAQLAEAAPAVRLVGEPRA
                     AERWAETLLALADRRAIHAIVAGRATRLLHEAAAQPPSAIGPRLARALSGSSPDETRY
                     AAGWLDGLLRDSGLLLVHDRSLSDEIDAWLAGLPGDRFVEALPLLRRTFAEYPESVRA
                     QLRRQLERRGGPAAPAAEGRAANFDPARAATMLPVVGRLLGVRLPVAVEER"
     gene            1853..2995
                     /locus_tag="PK829_03820"
     CDS             1853..2995
                     /locus_tag="PK829_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU40378.1"
                     /translation="MTGEERQRRWRLVLGAGQEEEGGGEGQGQPGRPAVTPPSLSPHD
                     QALDDALDALYGEARGGDLSKSMPDVARWLGDVRDYFPEPVVEIMQRDARQRLSTRQL
                     VEHPELLAEVEPDAALAAQLLALRKVMPERTLDTARQVVARVVAELTRRLEYPLRQAA
                     TGSLNRATRRHRPRKLQEINWTRTVQANLRHYQPAQGTIVPERLVGYGRRQPALRDVI
                     LLVDASASMATSVVYAGVAAAVLASLPSLAARLVLFDTAVADVSDQLADPVAMLFGLR
                     LGGGTNIDRALTYARGAISRPRDTVVVLISDLFEGGSKEGVVRQVGELLADGVAVIVL
                     LALSDGGAPRYDVALAQQLADLGAPAFACTPEEFAAVMGQALNGRD"
     gene            complement(3092..4132)
                     /locus_tag="PK829_03825"
     CDS             complement(3092..4132)
                     /locus_tag="PK829_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOU40379.1"
                     /translation="MDYKDYYKTLGVKKNAGADEIKKAYRKLARKYHPDVNPGDKSAE
                     ERFKDINEAYEVLSDEDKRGKYDRFGAEWQQFSRAGGRPEDFNWNQWGGAPGGAPGAG
                     GQYRSVSPEEFEQMFGRGAGGGGFSDFFEALFGMGGRGGATRTGFGGSYSGSNADFGD
                     LFGDAGGRAAAPSPDQEHPIQITLEEAFQGTTRTLQWEGGRRLEARIPRGVRTGSRVR
                     LSGQAGTGDGRAGDLYLKVEVLPHSVYERDGDNLRLTLPVDLFTVLLGGKVSVPALDK
                     SVNLNIPAGTRNGKVFRLARLGMPKLRQPDERGDLYVTVAVELPRHLSDEEKRLVGEW
                     QAVRQNAASDER"
     assembly_gap    4428..4527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4672..5460)
                     /locus_tag="PK829_03830"
     CDS             complement(4672..5460)
                     /locus_tag="PK829_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU40380.1"
                     /translation="MANINRWTPGRLSPWDRDLFDEMDRVFSEPFFGRRWDNERTSNW
                     GLPLDVSEREDKYIVRASIPGINPDNIDLSISDNMLTIRGNMSQSSDDESENYVLRER
                     RMGNFTRSVTFPTAIDANAAEASCEHGVLTIQLPKAATARPRRISIGGGAPGRQTIEA
                     QSGMGQTQTTTGTTGTAARMNQTSNGDQTQSGRAAGSEGWPEGQARPGRPSTETNRQS
                     QHWTEGQGTTAGEPKSEGWVEGQGTTANEPKSEGWTEGMDNTQS"
     gene            5955..8123
                     /locus_tag="PK829_03835"
     CDS             5955..8123
                     /locus_tag="PK829_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU40381.1"
                     /translation="MTWLITHKPAYLSDFMDLPRDLRNHVTAALRELERDPLTLRGDT
                     IKKLRGYENVYRYRLGDFRLIYAVAARAQVLNLLAIGPRGNIYRRFNYSGWDVPGAAA
                     TFDPELAQLQEWEEHPEWFGGVLLPRPLSPELLARWRVPAEHHAALMACRTEEELLGA
                     TDRGVPTDVVERVMNGLFPPAAAQLAAQPDLALFDPEDLVRYAEGTLAGFLLRLDESQ
                     KPLQHWALRGPTLVKGGPGSGKSTVALYRVRAVVEHHQQTTGRLPTVLFTTYTNSLTH
                     ASESLLRQLLTGLVPLAHDGKLPKEIRVSTLHKTAAWIANTGGTVTVATPADQQEALR
                     VARSAFGRALFGEAERARVGALTADLRDDYLLEEFEWAIEGQDCRDEAAYLAADRAGR
                     GIPFGRERRAAVWQLYEVYRDYLRDRGLCAWGQLTQLAIDRVRSGAFTRRWDYVIVDE
                     AQDLRPAALALAVELCREPGGVFLTADANQSLYHRGFRWNRIHAALQVVGRTRMLTRN
                     YRSTRQIAVAAAEILSGPDADEEAVRQEYIHSGPRPVVYAAPNTTAMWRWIFERIVGA
                     ARELRLPANAAAVLVPSHDVGELVAGALRDHRLPAQFMNSQTFDPDAPGVKVTTLHAA
                     KGLEFPIVVVAHVEAGRLPRETPATGPDEVAAFLEEQRRLLYVGCTRAMRRLYVTFDQ
                     AMPSPFVTGLGEERWEWWRTADGRQTAADGQPPTTAPRPY"
     gene            8164..>8358
                     /locus_tag="PK829_03840"
     CDS             8164..>8358
                     /locus_tag="PK829_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOU40382.1"
                     /translation="MLTHVVLIKLRDRSPEAIAAVRAHLDTLAGNIPSLRAIEVGTDV
                     LRSGRSFDLALVARFDDLAGL"
BASE COUNT         1215 a         2852 c         2840 g         1351 t
ORIGIN      
        1 aactcttcgt tgccgtgctg gagatggcca cggccctgcg gcgggcggcc gaagccgccg
       61 gcacggccac ccacgtcgct cccttgccgc cggagcaggc ggccgcggcc acccgcctgg
      121 ccgagcagcg cgaggcggcc atgcgtcaga gcatccgcgc cgccgcggcc gccggccgcg
      181 accgcatcgc cgccgtctgt ggcacgtggc acgccccggc gctgaccgcc gccctcgaca
      241 cgccgcccga cgccgacgct gccctactgc gtgacctgcc ggccatcgaa gtggccgcca
      301 cgtgggtgcc gtggacttac agccggctga cacaggccgg tggctatggc gcgggcgtgg
      361 cctcgccggg ctggtatgac catctctggg cgatgggcga ggccggcggc gcgttccagc
      421 gcaacgtcgc cgctgtctgg ctggccaacg ccgcccggct gctgcgcggc gagggcttcg
      481 acacctcgcc cggccacctc atcgaggccg tgcggctggc cgaggcgctg gcggcgctgc
      541 gcgagcggcc cttcccggcc ctggaggaac tgggcgaggc ggctcagagc gtcctctgcg
      601 gcggcgacgc cgagccgatg gccctcatcc gccagcggct ggtggttggc gagcgcatgg
      661 gcatcgtccc gccggacgtg cccgctgtgc ccttacagcg cgacctgcgc gcccaacaga
      721 cgcggctgaa gctgccgccg gagccagagc ctgtcgcgct gagcctcgat ctgcgccagg
      781 agatagacct ggagcgcagc cgcctgctcc accgcctggc cctgctggac atcccctggg
      841 gcgagacggc cacgaccaag ggacaggtgg gcacgtttgc cgagcgatgg caattgcagt
      901 ggcggccgga gttgtcgctg cgggtcatcg cggcggccat gtggggcaac accgtccgcg
      961 acgccgccgc ggcccacacc ggcgacctgg ccgccagaac cgacgccctg ccagcgctca
     1021 tcgccctcat cgaccgggcc gtgctggccg atctgccgga ggccatcccc gccgtgttga
     1081 cacgcatcga ggagctgtcg gccggtagtg cggacgtcgt ccacatgttg gcggcgttgc
     1141 cgccgctggc ccaggtgttg cgctatggcg ggctgcgaca tacggccgag cacctgcccc
     1201 tgctgcggcg cgtctttgac catctcctga cacgggcctg cctgtcgctg ccgcgcgcga
     1261 cggtagccat cgacgacgaa gccgcgcgcg acctggccgc gcagctggcc gaggccgccc
     1321 cggccgtgcg tctggtgggc gagccgcggg cggccgagcg ctgggcggaa acgctgctgg
     1381 ccctggccga ccggcgcgct atccacgcca tcgttgccgg gcgggccacg cggctgctgc
     1441 acgaggctgc cgcccagccg ccgtcagcca tcggcccacg actggcgcgg gcgctttccg
     1501 gcagctcgcc cgacgagacg cgctacgccg ccggttggct ggacgggctg ctgcgcgaca
     1561 gcggactgct gttggtgcac gaccgttccc tgtcggacga gatcgacgcc tggctggccg
     1621 ggctgccggg cgaccgcttc gtggaggcgc tgccgctgct gcggcggacg tttgcggaat
     1681 acccggaaag tgtgcgcgcg caactgcgcc gccaactgga gcggcgcggc ggcccagccg
     1741 cgcccgccgc cgaggggcgc gcggccaact tcgacccggc gcgggcggcg acaatgttgc
     1801 cagtggtcgg ccggctgctg ggcgtgcggc tgccggtggc cgtggaggag cgatgacggg
     1861 cgaggagcgg caacggcgct ggcggctggt gctgggcgcg ggccaagagg aggaaggggg
     1921 cggcgagggg caggggcagc cgggccgccc cgccgtgacc ccgccgtccc tttcgccgca
     1981 cgatcaggct ctcgacgacg ccctcgacgc gctctatggc gaagcgcgcg gcggcgacct
     2041 gagcaagtcg atgcccgatg tggcccgctg gctgggtgac gtacgcgact acttcccgga
     2101 gccggtcgtg gagatcatgc agcgcgatgc ccggcagcgg ctatcgacgc gccaactggt
     2161 ggagcatccg gagctactgg ccgaggtgga gccggacgcg gcgctggcgg cgcagctgct
     2221 agccctgcgc aaggtgatgc cagagcgaac gctggacacg gcgcggcagg ttgtcgcccg
     2281 tgtcgtggcc gagctgacgc ggcggctgga ataccccttg cggcaggcgg ctacgggcag
     2341 cctcaatcgc gccactcgcc gccaccggcc gcgcaagctc caggagatca actggacgcg
     2401 caccgttcag gccaatttgc gccactacca gcccgcccag ggcaccattg tgccggagcg
     2461 gttggttggc tacggccgcc gccagccagc cctgcgcgat gtcatcctgc tggtggacgc
     2521 cagcgcttcg atggccacgt cggtggtcta tgccggcgtg gccgcggccg tgctggcctc
     2581 gctgccgtcg ctggcggcgc ggctggtgct gttcgacacg gctgtggccg acgtcagcga
     2641 ccagttggcc gacccggtgg cgatgctctt cggtctacgg ctgggcggcg gcacaaacat
     2701 cgaccgtgcc ctgacctatg cccgcggggc catcagccgg ccgcgcgaca ccgttgttgt
     2761 gctcatcagc gacctatttg aggggggcag taaggagggg gttgtgcgcc aggtgggcga
     2821 gctgctggcc gacggcgtgg ccgtcatcgt cctgctggcg ctaagcgacg gcggcgcgcc
     2881 gcgctacgac gtcgctctgg cgcaacagct ggccgacctg ggcgcgccgg ccttcgcctg
     2941 cacgccggag gagttcgcgg cggtcatggg gcaggcgctg aatgggcgcg actgacctta
     3001 ccgccaggac gcgaagccgg caggaacgcc aggagacgta gagggccgac acgcgggtct
     3061 gtcccttccg acgcagcgag taacgccaac gtcaccgctc gtcgctcgcg gcgttctgcc
     3121 gcacggcctg ccactcgccc accagccgct tctcttcgtc cgacaggtgg cgcggcagtt
     3181 ccacggccac cgtgacgtac agatcaccgc gctcgtccgg ttggcgcagc ttgggcatgc
     3241 ccagcctggc caggcggaag actttgccgt tgcgcgtgcc ggcgggaatg ttgaggttga
     3301 ccgacttgtc gagcgcgggc acgctgacct tgccacccaa caggacagtg aacaagtcca
     3361 cgggcagggt caggcgcagg ttgtcaccgt cgcgttcata gacggagtgg ggcagcacct
     3421 cgaccttgag gtaaaggtcg ccggcgcggc cgtcgcccgt gccggcctgg ccgctcaggc
     3481 gcacacgcga gccggtgcgc acacctcgcg ggatgcgcgc ctccagccgc cggccgccct
     3541 cccattgcag ggtgcgcgtc gtgccctgga aggcttcctc cagggtgatc tggatgggat
     3601 gttcctggtc aggcgacggg gctgcggccc ggccgccggc gtcgccgaag agatcgccga
     3661 agtcggcgtt gctgccgcta tagctgccgc caaagcccgt ccgcgttgcc ccgccgcggc
     3721 cgcccatgcc aaagagcgct tcgaagaagt cggagaagcc gccgccgcca gccccacggc
     3781 cgaacatctg ctcaaattcc tccggcgaga cagagcgata ctgccctccg gccccgggcg
     3841 cgccacccgg cgcgccgccc cactggttcc agttaaagtc ctccggccgg ccgccggcgc
     3901 gtgagaactg ctgccactcc gcgccaaagc ggtcgtactt gccgcgcttg tcctcgtcgg
     3961 aaagcacctc gtaggcctcg ttgatgtctt tgaagcgttc ttcggcgctc ttgtcgcccg
     4021 gattgacgtc agggtgatat ttccgcgcca gcttgcggta ggccttcttg atctcatcag
     4081 cgccagcgtt tttcttgacg ccaagcgtct tgtaataatc cttataatcc attgccattg
     4141 cgataccaca ggcggcgcat ctgtgccgga gagcaatgct cccgcgccgc aacctgtatt
     4201 atgcctcaga cgagtcagaa ttgagtaaga ctggcccgtt ctgcgtggcg cgattctcct
     4261 gaatcgcgtc ttatggctgg tgctgtggca gaacgcgcca cgcgaggatc gcgattcgga
     4321 gaatcgcgcc acgtgaggat cgcgattcgg agaatcgcgc catgcgagga ttgcgattcg
     4381 gagaatcgcg ccacgcgagg atcgcgattc ggagaatcgc gccacgcnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt cggagaatcg cgccacgcga ggatcgcgat
     4561 tcggagaatc gcgccacgcg agaatcgcgc cacgcgaggg ctggggcaac aaaaagaggc
     4621 agccccgcgg ctgcctctgc gtcgctgtca gcaatggctg gcggctggcg cttacgactg
     4681 cgtgttgtcc atgccttccg tccagccttc gctcttgggt tcattggccg tcgtgccctg
     4741 gccctcgacc cagccctcgc ttttcggttc gccggcggtc gtgccctggc cttcagtcca
     4801 gtgctggctc tggcggttgg tctcggtaga cgggcggcca gggcgggcct ggccctccgg
     4861 ccagccctcg ctgccagcag cacggccgct ctgggtctgg tcaccattac tcgtctggtt
     4921 catacgcgcg gccgtgcccg ttgtgcccgt ggtggtctgg gtctggccca tgccggattg
     4981 agcctcgatg gtctgccggc cgggcgcgcc gccgccaatg ctgatgcggc gcgggcgggc
     5041 cgtggcggcc ttgggcaatt ggatggtcag cacaccatgc tcgcaactgg cctcggccgc
     5101 gttcgcgtcg atggccgtcg gaaaggtgac gctgcgggtg aagttgccca tgcgacgctc
     5161 gcgcagaacg tagttctcgc tctcgtcgtc actcgactgg ctcatgttgc cgcggatggt
     5221 cagcatgttg tcactgatgg acaggtcaat gttatcgggg ttgataccgg ggatggaggc
     5281 gcggacaata tacttgtctt cgcgctcgga tacgtccagc ggcaggcccc agttgctcgt
     5341 gcgctcgttg tcccagcgcc ggccgaaaaa cggctcggaa aagacgcggt ccatctcatc
     5401 gaacaggtca cgatcccacg ggctgagccg accgggagtc caacggttaa tatttgccat
     5461 tcctgaatac ctccagttag tgtttaacct tcaacagtta ttgacagaac acaggtgggc
     5521 gtcgaggatg gggagggatg caggctacac cgccagaatt atgttaccca ccgctatcta
     5581 ccctaacgcg cacgtgtaag aatgctgtta gcaatgggtt ggcatcacat tagatatagc
     5641 cagtgggcaa tagcctggcg tctgtcggcc gggaccggcc gccggcttat ggcgttacga
     5701 ggctttgcca gagtcgtcga gcgaccattg acctgaccgg tggacggcca gtcgttctgc
     5761 ggtgacagta ccatgcgtgg ccgcccatcg gtcaggtcgg cgcgccgggg gtgactttgg
     5821 ggaaaccctg atggcgtcat ttgtttcgta agtatgcaac agtatattag acgctagcgg
     5881 tcggcttgat ctcgccggcc ctatcgttcg ctccgcggca tccgcgcggc caatctagat
     5941 gattctgccc tgctatgacc tggcttatca cccacaaacc cgcctacctg tccgatttca
     6001 tggatctacc gcgcgatctg cgcaaccacg tcaccgcggc gctgcgcgag ttggaacgcg
     6061 acccgctgac gctgcgcggc gacacgatca agaagctgcg cggttacgaa aacgtctacc
     6121 gctaccggct gggcgatttc cgcctaatct atgccgtggc cgcgcgggca caggtgctca
     6181 acctgctggc cattgggcca cgcggcaaca tctaccgccg cttcaactat tccggttggg
     6241 atgtgcccgg cgcggcggcg accttcgacc cggaactggc ccagttacag gagtgggagg
     6301 agcatcccga atggtttggc ggcgtgcttc tgccccgccc cctttctccc gaactgctcg
     6361 cccgctggcg tgtgcccgcc gaacaccacg ccgcgctgat ggcctgtcgc accgaggagg
     6421 aattgctggg ggccaccgac cggggcgtgc ccaccgacgt cgttgagcgc gtcatgaacg
     6481 gcctgtttcc cccggccgcc gcgcagctgg ccgcgcagcc cgacctggct ctcttcgatc
     6541 ccgaagacct ggtccgctat gccgagggca cactggccgg tttcctgctg cggctcgacg
     6601 agagccagaa gccattgcag cattgggcgc tgcgcgggcc gacgctggtc aagggcggcc
     6661 ccggctcggg caagtcgacc gtggctctct atcgcgtccg cgctgtcgtt gaacatcacc
     6721 agcagacgac tggccggctg cccactgtcc tctttaccac ctataccaac tcgctgaccc
     6781 acgccagcga gtcgctcctg cgccaactgc tgaccggtct ggtccctctg gcccacgacg
     6841 gcaagttgcc caaagagatc cgcgtcagca cgctgcacaa gacggccgcc tggatcgcca
     6901 acacgggcgg cacggtaacc gttgccacgc cggccgacca gcaagaggcg ctgcgcgtgg
     6961 cccggtcggc cttcggccgg gcgctgttcg gcgaggcgga gcgggcgcgc gtgggcgcgc
     7021 tgacagccga cctgcgcgat gactatctgt tggaagagtt cgagtgggcc attgagggcc
     7081 aggattgccg cgacgaggcc gcctatctgg ccgcggaccg ggccgggcgg ggcatccctt
     7141 tcggccgcga acgacgggcg gccgtctggc agctctatga agtctaccgc gattacctgc
     7201 gcgaccgcgg cctgtgcgcc tggggccagc tgacgcagct ggccatcgac cgcgtacgca
     7261 gcggcgcgtt tacccgccgc tgggactacg tgatcgtcga cgaggcgcaa gacctgcggc
     7321 cggcggcgct ggctctggcg gtggagctgt gtcgcgaacc gggcggcgtc tttctgaccg
     7381 ccgacgccaa ccagtcgctc tatcatcgcg gcttccgctg gaaccggata catgcggcgc
     7441 tgcaagtggt cgggcgcacg cggatgctga cgcggaacta tcggagcacg cgccagatcg
     7501 ccgtcgccgc agccgaaatc ctgtctggcc ccgacgccga cgaagaagcc gtccggcagg
     7561 agtacatcca cagcgggccg cggccggtcg tctacgccgc gccgaacacg acagccatgt
     7621 ggcgctggat cttcgagcgt atcgtcggcg cggcgcgcga gctgcggctg ccggccaatg
     7681 ccgcggctgt tctcgtgccc tcgcacgacg tcggcgaact ggtggccggc gccctgcgcg
     7741 accaccgcct gccggcgcag tttatgaata gccagacgtt cgacccggac gcgccggggg
     7801 tcaaggtgac aacgctgcac gcggccaagg ggctggagtt tcccatcgtg gtcgtcgccc
     7861 acgtcgaggc tgggcggctg ccacgcgaga cgccggcgac cggccccgac gaggtggctg
     7921 cctttctgga ggagcagcgg cggctgctct acgtcggctg cacgcgggcc atgcgccggc
     7981 tctacgtcac cttcgatcag gccatgccgt cgccattcgt gaccgggctg ggcgaggagc
     8041 ggtgggaatg gtggcggacg gccgacggcc gacagacggc ggccgacgga caaccgccga
     8101 ccacggctcc acggccctac tgacccactg gcttcttccc aaaaccacgc tatcctcgct
     8161 cctatgctca cccacgtcgt cctcatcaag ctcagagacc gaagcccgga ggccatcgcc
     8221 gccgtccggg cgcacctgga cacgctggcc ggcaatatcc ccagcctgcg tgccattgaa
     8281 gtgggcacgg acgtgctgcg ctccggccgc tcgttcgacc tggccctcgt cgcccgcttc
     8341 gacgacctgg ccgggctg
//
LOCUS       DAOSMQ010000074         8339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8494_length_8339_cov_3.970787, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000074 DAOSMQ010000000
VERSION     DAOSMQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8494_length_8339_cov_3.970787"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK829_03845"
     CDS             complement(<1..221)
                     /locus_tag="PK829_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40383.1"
                     /translation="MTLNLVAIGVLIVAAALYAALVPGRWRGWAVLLGSIVALFWLQA
                     PLAPRFADFLLPSATVALAVAGWWLSRPG"
     gene            complement(247..1308)
                     /locus_tag="PK829_03850"
     CDS             complement(247..1308)
                     /locus_tag="PK829_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40384.1"
                     /translation="MDLSGFLGRLARRALLAVLPAAVALLAGCGRVAEPLRGAVAPRP
                     TAAVAALLPFAPTASVVPPTPAPTLPSPTPTPTPTATGTPAPTATATSLAPPTATPDP
                     ARQINGVPFEAIAVLPPETAAHVAEIIARGRELGRNPRAFSKLGDSAVLTESNLTRFD
                     NGGYTLGLYAFLQPTIDAFTGSWGRYGAGARVALTTVGALDPMWARPEYCAAGEDPLQ
                     CEIRLNNPAVMLIRLGTNDGRAASYEANMRVIIDACLEQGVVPVLGTKADRFEGDDSI
                     NAATRRLAEELRLPLWDFDRVADTLPNRGLNDDHAHLTTTARNDYTDPETLTRGYPVS
                     DLTALVMLDALRALGLGGE"
     gene            complement(1455..1847)
                     /locus_tag="PK829_03855"
     CDS             complement(1455..1847)
                     /locus_tag="PK829_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40385.1"
                     /translation="MKKWFIACLLVATLTLLIACGGSNAGDSDADPAARAVEAYLTAK
                     VSGDADAIRAGLCSEMESVLERELHTFETVSGAHLEDMACASDGADVVRCTGQIVALY
                     GTEETTFPLGAYRVVEEDGVYRWCGEAE"
     gene            1949..3178
                     /locus_tag="PK829_03860"
     CDS             1949..3178
                     /locus_tag="PK829_03860"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU40386.1"
                     /translation="MLTQYVVAPLARLAAAVERRLALPGDDETLRRRRVVAFFAGLAG
                     VNTAAVFAVLYWAGGAPLLAWLYILTVAVSGATLGYLLLRPRAYTWCVLITTTYVTIH
                     PWIVGLASGGFRSGLLPMLWALVGPAGSLLLIGVRPALFHAGLYVVMALGSVFLDPWA
                     AAHTPALPQNLRLALSAISALVPGMMVLFISLFLFGQVERARQQADRLLRNVLPEPIA
                     NQLKQSPATIADSHDEVTVLFADIVGFTKMSSHAAPANVVRLLNAIFSDFDELADRHG
                     LEKIKTIGDAYMVVGGLTQPLPDHVAAVVAFAVDMLDIIGHHRGPNGEPIGLRIGIHT
                     GPIVAGVIGRRRLIYDLWGDTVNTASRMESYGLENLIQVTPAVVARLNGRYHFASRGP
                     IDIKGKGLMTTYILRPN"
     gene            complement(3271..4131)
                     /locus_tag="PK829_03865"
     CDS             complement(3271..4131)
                     /locus_tag="PK829_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU40387.1"
                     /translation="MGTFAFVVLMLVFIIAIAALAISVRVIKQFEKGLVIRLGRYSHT
                     ADAGVVFLVPFIDSLIRVDMREALINVLPQQVITADNVVVTVDAVIYYKVVDPVKAEF
                     EIQNFNFAATTLAQTNLRNLIGDKQLDETLTARDVINASLREVLDEATNPWGVKVTKV
                     ELQKIDPPADITTAMSKQMKAEREKRAVILEAEGVRQADILRAQGEAQARLLRAEAEA
                     KSIEVVALAAERNFTDRARELRKLEVAQAVLSGNQTKFIIPAGSDIINVLGLDSDGHN
                     NIVPVRRKSE"
     gene            complement(4145..4579)
                     /locus_tag="PK829_03870"
     CDS             complement(4145..4579)
                     /locus_tag="PK829_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOU40388.1"
                     /translation="MTIPQLFVIAGLLLILAELLVGIQAGFDLVLIGSILFVSGFVGM
                     FTNSVPLMLVLATVLSILYIAVGRQKIREKVAVVTKKTNIDKLVGATGTVVRSVTPDT
                     AGLVRVNDEDWRASAEEILYERDTIVVEGIEGVTLIVRKLSK"
     gene            complement(4970..7648)
                     /locus_tag="PK829_03875"
     CDS             complement(4970..7648)
                     /locus_tag="PK829_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOU40389.1"
                     /translation="MKKSIFQPSIVPVLAPEGATDNDIPFIYMASGGQQPRRRIIGQR
                     RRPGSDRPSSGRADAPHRDDRPASSGGGYSQPTGGGYTGGGYSGGGLGGFPGGTRGAA
                     GGGGILLFLCAAIAYFLFGGGGDGGGILDTGQVQQDTSGQSSQVEDLGSLADEVSSGT
                     TGGLPTVAPLGGQTSSTTFQGSGGLTAAANSGEVTGAAAASLPAAANSTVTDGQTWTI
                     LLYQDADDKVLEQDIFIDFNEAERVGSTDRVKLVAQMDRYQGGFSGDGNWTDTRRYLL
                     QQDDNLDSIASPYESIGEVDMSDPQELVNFVTWGIQNFPADKYVLIMSDHGMGWPGGW
                     TDAEPRSVRRSTTPLAQAIGDAMYLQDIDAALQTIRDQTGLDAFELIGMDACLMGHME
                     VFTMLAPHARFAVASQEVEPAVGWAYTAFLSQLAQNPDVTGRELGQWIVDTYIVGDQR
                     VQDPQARAAFVGGRGATAEQVAAQLEQSVTLAAVDLSQMPAANDGLNRFALYLRGMDQ
                     RQVAQARSNAQSFTSIFGQQVPASYIDLPHFAALLVRQTGDAQLRSVVEDMFGAFQNA
                     VIAEKSGRQKPGATGLSIYFPNSQLYASAAAGPQSYIPITERFANVSLWDDYLTYHYT
                     GRSFQDNAGSLAIPDRGATITAPGAGAISLSPLSVSDTTVAPGGSILMSADVDGDNIG
                     YIYLFAGFYDRDANSVNVIDTDYIDSGDTREMDGIFYPDWGEGAFTLEFEWEPIVFAI
                     DDGQNQVLALLQPQTYGAAPEDAVYAVDGLYTFSTGEQRFARALFSNESLQQVITYQT
                     TDNALPSPNDVKNSPRVVTPQAGDQFTVLELWYDLDDTGQLVDAAYEPGGTLTFGQGL
                     WTYTVQDAPAGDYTIGFIVEDLDGNRTETYADVTVQ"
     gene            7989..>8339
                     /locus_tag="PK829_03880"
     CDS             7989..>8339
                     /locus_tag="PK829_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40390.1"
                     /translation="MIGDNPTTPGDALDWPALGRVVLKALLLFALVNAAFAAVRPLEA
                     LGRLSLYNSLFPGRARLPYGEVIAADYNLTLNNLPAMFAAHALARPKDADEYRVVLIG
                     DSGTWGWFLANDDTL"
BASE COUNT         1388 a         2815 c         2655 g         1481 t
ORIGIN      
        1 tgccccggcc gcgacagcca ccagccggcc acggccaggg ccaccgtcgc gctgggcagt
       61 agaaagtcgg cgaagcgcgg ggccagcggc gcttgcagcc agaacagggc cacgatactg
      121 cccaacaaca ctgcccagcc gcgccagcgc cccggcacga gcgcggcata cagcgccgcc
      181 gcaacgatca gcacgccgat ggccaccagg ttgagcgtca cgcggcgcgc cccccgctca
      241 tgcctctcac tctcctccca accccagcgc ccgcagcgcg tcgagcatca ccagcgccgt
      301 caggtcgctg accgggtagc cgcgggtcaa cgtctccggg tcggtgtagt cgttgcgcgc
      361 ggtcgtggtc aggtgggcgt ggtcgtcgtt caggccgcgg ttgggtaggg tgtcggccac
      421 gcggtcgaaa tcccacagcg gcaggcgcag ctcctcggcc agccggcgcg tggccgcgtt
      481 gatgctgtca tcgccctcaa agcggtcggc cttggttccc agaacaggca caacgccctg
      541 ctccagacaa gcgtcgatga tgacgcgcat gttggcctcg tagctggccg cccggccgtc
      601 gttggtgccc aggcggatga gcatgaccgc ggggttgttg agccggattt cacactgcaa
      661 tgggtcttcg cccgcggcgc agtattccgg ccgcgcccac atcgggtcga gcgcgccgac
      721 ggttgtcaat gctacccgcg ctccggcccc atagcgcccc cacgagccgg tgaaggcgtc
      781 gatggtcggt tgcaggaagg cgtagaggcc gagagtgtag ccgccgttgt cgaaacgggt
      841 caggttgctt tcggtcagca cggcgctatc gcccagcttg gagaaggcgc gcgggttgcg
      901 gcccagttcc cggccgcggg cgatgatctc ggccacgtgg gcggctgttt ctggcggcag
      961 cacggcaatg gcctcgaagg gcacgccgtt gatctgtcgc gccgggtcgg gcgtagcggt
     1021 cggcggcgcg agcgaagtgg ctgtggcggt tggcgcgggt gtgcccgtgg ccgtcggcgt
     1081 tggggtcggc gtgggcgagg gcagcgttgg cgcgggcgta ggcggcacaa ccgaagccgt
     1141 gggcgcgaag ggcagcaggg cggccacggc ggctgtcggc cgcggcgcga cggccccccg
     1201 cagcggctcc gcaaccctgc cgcaaccagc cagcagggcg acggccgccg gcaagaccgc
     1261 caacagcgct cgcctggcca gtcgccctag aaaccctgat agatccatgg cggggcgctg
     1321 tccgtggcca cgatcaggag caggatgatc agcaggcaca ggagaatggc aaggatgttt
     1381 tcgcgtgcgc gcatgagagc agagccatca gcagttagat ctcagaggtc taactgaaca
     1441 ccggcagttc tttctcactc ggcctcgcca caccagcggt agactccatc ctcctccacg
     1501 acgcgatacg cgcccagggg gaaggtcgtt tcctcggtgc catagagggc gacgatctgg
     1561 cccgtgcagc gcacgacgtc cgcgccgtcg ctggcgcagg ccatgtcctc caggtgcgcg
     1621 ccgctgaccg tctcgaaggt gtgcagttcg cgctccagaa cgctctccat ctccgagcac
     1681 agcccggcgc ggatggcgtc agcgtcgccg ctcactttgg ccgtcagata ggcttctacg
     1741 gcgcgagcgg ccgggtcggc gtcgctgtcg ccggcgttgc tgccgccgca ggctatcagc
     1801 agcgtcagcg tagctaccag caggcaggct ataaaccact ttttcatcaa cgcttcctcg
     1861 atccccttct cttggccaaa ggggacgggg gaattatgcc gcggcttaga cgcttaggcc
     1921 acacaggcta taatctgtcc acctgaccat gctcacccaa tacgtcgtgg ccccgctggc
     1981 ccgccttgcc gcggctgtag aacgccgtct ggccctcccc ggcgacgatg agacgctgcg
     2041 ccgccgcaga gtagtggcct tctttgccgg gctggccggc gtgaacaccg ccgccgtctt
     2101 cgccgtgctc tattgggctg gcggcgcgcc gctgctggcc tggctctaca tcctcaccgt
     2161 cgccgtttcc ggggcgacgc tgggctacct gctgctccgg ccgcgcgcct acacgtggtg
     2221 tgtgctcatc accaccacct acgtcaccat tcatccctgg atagttggtc tggcgtcggg
     2281 cggcttccgg tcggggctac tgcccatgct ctgggcgctg gtcggccccg ccggcagtct
     2341 gctgctaatt ggcgtgcgtc cggcgctgtt ccacgccggc ctctacgtcg tcatggccct
     2401 cggctcggtg ttcctcgacc cgtgggccgc ggcccacacg cccgccctgc cccagaacct
     2461 gcgcctggcc ctcagcgcta tcagcgccct cgtgccgggc atgatggtgc tgttcatcag
     2521 cctctttctc tttggccagg tcgaacgcgc ccgccagcaa gccgacaggc tgctgcgcaa
     2581 cgtcctgccg gagccgattg ccaaccagct caagcaaagc ccggccacca tcgccgacag
     2641 ccacgacgag gtcacagttc tctttgccga catcgttggc ttcacgaaga tgagttccca
     2701 cgccgcgccc gccaatgtcg ttcgccttct taatgccatt ttctccgact tcgacgagct
     2761 ggccgaccgc cacgggttgg aaaagatcaa gactatcggc gacgcctaca tggttgtcgg
     2821 cggcctgacg cagccgcttc ccgaccacgt cgcggccgtc gtcgccttcg ccgtggacat
     2881 gctcgacatc attggccacc accgcggccc caacggtgag ccaatcggcc tgcgcatcgg
     2941 catccacact ggccccattg tcgccggggt catcggccgc cgccgcctca tctacgacct
     3001 ctggggggac acggtgaaca ccgccagccg catggaatcg tatgggctgg agaacctcat
     3061 ccaggtgacg ccggccgtcg tcgccaggct gaatggccgc taccacttcg cgtcgcgtgg
     3121 gccgattgac atcaagggca aggggctgat gaccacctac atcctgcggc ccaactgagc
     3181 accacgcccc gccccggcga cggctgccca acaaaaaggc ccggtcagcg tatgaccggg
     3241 ccttgccctt gacttgccgg cgaccagcgg ctactccgac ttacgccgca ccggcacaat
     3301 gttgttgtgc ccgtcgctgt ccaggcccag gacattgatg atgtccgacc cggccgggat
     3361 gatgaacttg gtctggttgc ccgacagcac ggcctgggcc acttccagct tcctcaattc
     3421 gcgggcgcgg tcggtgaagt tgcgctcggc cgccagggcc accacctcga tcgacttggc
     3481 ctccgcctcg gcccgcagca gtcgcgcctg ggcctcgccc tgcgcccgca ggatgtcggc
     3541 ctgccgcacg ccctcggctt ccaggatgac ggcgcgcttc tcgcgctcgg ccttcatctg
     3601 cttgctcatg gccgtggtga tgtcggccgg cgggtcgatc ttttgcagct cgactttggt
     3661 caccttgacg ccccacgggt tggtggcctc gtccagcacc tcgcgcagcg aggcattgat
     3721 cacatcgcgg gcggtcagcg tctcatcgag ttgcttgtcg ccgatcaggt tacgcaggtt
     3781 cgtctgcgcc agcgtcgtgg cggcgaagtt gaagttctga atctcgaact cagccttcac
     3841 cgggtcgacg accttgtaat agatgaccgc gtcgacagtc acgaccacgt tgtcggcggt
     3901 gatgacctgc tgcggcaaaa cgttgatcag cgcctcgcgc atgtcgacgc ggatcaggct
     3961 gtcgatgaac ggcaccagga agacgacacc ggcatcggcc gtgtggctat agcgccccag
     4021 gcgaatgacc agccccttct caaattgctt gatgacgcgc acgctgatcg ccagcgcggc
     4081 gatggcgatg atgaagacca gcatcaaaac gacaaaggca aaagtaccca tgtttctcct
     4141 gcgattactt gctcaacttg cgaacgatga gggtcacacc ctcgattccc tcgaccacga
     4201 tagtatcgcg ttcgtagagg atctcctcgg ccgaggcgcg ccagtcttcg tcgttgacgc
     4261 gcaccagtcc ggcggtgtcc ggcgtaacgc tgcggacgac cgtgccggtc gctccgacca
     4321 gcttgtcgat gttggtcttc ttggtcacga cggcgacctt ttcgcggatt ttctgacggc
     4381 cgacggcgat gtagagaata ctcaacaccg tcgccaatac cagcatcagc ggcacgctgt
     4441 tggtgaacat gccgacaaag ccggacacga acaggatcga gccgatgaga accaggtcga
     4501 agccagcctg gatgccaacc agtagctcgg ccaagatcag caacagcccg gcaatcacga
     4561 ataattgggg aattgtcact acaccgtcca tagttcctgc cctggcgcat acgatgctgc
     4621 acaggcacgc cacacactgt ttgacacgct gggcgatcgc ttctaactcg gatcaccctt
     4681 gaaccatcca cagtcctata cgcgccctcc ggccacaagt tgcgccagat atgcgctcag
     4741 tgatgagtat aacaggtttt ccccaaatat ggggccgtcc aggcccgtgg cgcgattctc
     4801 ccgaatcgcg tccttctgtc gtggtgcgcc atggcgtaac cctcccgaag aacgcgattc
     4861 ggagaatcgc gccacagcgc cgccacaggg cgccacagcc caaaaaggcg ggtcacatga
     4921 cccgcctttg gcaaaaaaga cgttgcggcc ggcaaggcca cgacgcccgc tactgcactg
     4981 tcacgtccgc gtaggtctcg gtgcggttgc catccaggtc ttcaacgatg aagccgatgg
     5041 tgtaatcgcc ggccggagcg tcttgcacgg tataggtcca tagaccctgg ccaaaagtca
     5101 gcgtcccgcc gggttcatag gcggcgtcga ccaactggcc tgtgtcatcc aggtcgtacc
     5161 acagctccag cacggtgaac tggtcgcctg cctgcggcgt caccacccgg ggcgaattct
     5221 tgacatcgtt gggcgagggc agggcattgt ccgttgtctg gtaggtgatg acctgttgca
     5281 gcgactcgtt gctgaagagc gcccgcgcaa agcgctgctc gccggtcgag aacgtataca
     5341 ggccatcaac cgcatagacc gcgtcttccg gggccgcgcc atacgtctgc ggctggagca
     5401 gggccagaac ctggttctgg ccatcatcga tggcaaagac gattggctcc cactcaaact
     5461 ccagcgtaaa cgcgccctcg ccccaatcgg ggtagaagat gccgtccatc tcgcgcgtgt
     5521 cgccactgtc gatgtaatcg gtgtcgatga cgttgaccga gttcgcgtcg cggtcataaa
     5581 agccggcgaa gagatagatg tagccgatgt tgtcgccgtc cacgtcggcg ctcatcagga
     5641 tcgacccgcc cggagcgacg gtcgtgtccg acacgctgag cggtgacagg ctgatggccc
     5701 ccgcgcccgg cgcggtgatc gtcgcgccac ggtcgggaat ggccagcgaa ccggcgttgt
     5761 cctggaagga gcggccggtg tagtgataag tcaggtagtc atcccacaag gaaacgttgg
     5821 caaagcgctc ggtaatggga atgtacgact gcggcccggc ggcggccgag gcgtacagtt
     5881 gcgagttggg gaagtagatc gacaggccgg tggcgccggg cttctgccgg ccgctcttct
     5941 cggcgatgac ggcgttctgg aacgcgccga acatgtcctc aacgacgctg cgcaactggg
     6001 cgtcgcccgt ctgccgcacc agcagcgcgg cgaagtgagg caggtcgatg tacgacgccg
     6061 gcacttgctg gccgaagatg ctggtgaagg actgggcgtt ggaacgcgcc tgggccacct
     6121 gccgctggtc catgccccgc aggtagaggg cgaaccggtt cagcccatcg ttggccgccg
     6181 gcatctggct cagatcgaca gccgccaggg tgacagactg ttccagttgc gcggctactt
     6241 gctcggccgt ggcgccgcgc ccgccaacga atgccgcccg cgcctggggg tcttgcacgc
     6301 gttggtcgcc aacgatgtag gtatcgacga tccactggcc cagttcacgg ccagttacgt
     6361 ccgggttctg cgccagctgg ctcaagaagg ccgtgtaggc ccagccgaca gccggctcca
     6421 cctcttgcga ggcgaccgca aagcgggcgt ggggagccaa catggtgaaa acttccatat
     6481 ggcccatcag gcaggcgtcc atgccgatca gctcgaaggc gtctaggccg gtctggtcgc
     6541 gtatcgtttg caacgccgcg tcaatgtcct gcagatacat ggcatcgcca atggcctgtg
     6601 ccagcggcgt tgtgctccgg cgcacggagc gcggctcggc gtccgtccag ccgcccggcc
     6661 agcccatgcc gtggtcggac atgatcagca cgtacttgtc ggctggaaag ttctggatgc
     6721 cccaggtgac gaagttcacc agctcctgcg ggtcggacat atcgacctcg ccgatggact
     6781 cgtaaggcga ggcaatgctg tcgaggttgt catcctgttg cagcagatag cggcgcgtgt
     6841 cagtccagtt gccgtcgccg ctgaagccgc cctggtagcg gtccatctgg gcgacaagtt
     6901 tcacccggtc ggtcgagccg acacgttcgg cctcgttgaa gtcaatgaag atgtcttgtt
     6961 ccagcacttt atcgtcggcg tcctgataga gaaggatggt ccacgtctgg ccgtcggtaa
     7021 cggtgctatt ggccgcggcc ggcagcgagg ccgccgccgc gccggtcact tcgccactgt
     7081 tggccgccgc ggtcagcccg ccgctgccct ggaacgtcgt ggacgacgtt tgcccgccaa
     7141 gaggggccac agtcggcaat ccgccggtgg tgccgctgga aacctcatcg gccaggctgc
     7201 ccaggtcttc gacctgcgag ctttgtccag acgtgtcctg ctgcacctgc cccgtgtcga
     7261 ggataccgcc gccgtcgccg ccgccaccga agaggaagta ggcgatagcc gcgcacagga
     7321 agagcaagat accgccgccg ccggccgcgc cgcgcgtgcc gccgggaaag ccgcccaagc
     7381 cgccgccgga gtagccgccg ccggtgtaac cgccacctgt gggctggcta tagcccccgc
     7441 cagacgaggc cgggcggtcg tcgcggtgcg gcgcgtcggc gcgcccactg ctgggccggt
     7501 cgctgccggg ccgccgccgc tggccaataa tgcgccggcg cggctgctgg ccgcccgagg
     7561 ccatatagat aaaggggatg tcgttgtccg tggccccttc gggcgccagg acaggcacaa
     7621 tggacggttg gaatatactc ttcttcatgg tctgcctcct tgctgacagc ctcatgttca
     7681 acgagtagct cagggaacag cgctcgcgga cggcctttcg gcggccggca cgggcgcttg
     7741 gcgcggtcgc tcgcgggccg ccagcatccc catgctaccc tctcagttta gcaattgtca
     7801 gggaaaaggc catgtatcct gtgtacacct ttggaggggc tggcggctgg aggccggccc
     7861 ctttcgtcgt cctctggcgc gctgacaggg cttttccaaa gtcacccccg gcgcaatggc
     7921 cgctagacga ctgtggcaaa gccctgggcg cacttgtgcc ctggccgcgg atggggcatc
     7981 attcggacat gatcggcgac aaccccacca cgcccggcga tgcactggac tggcccgccc
     8041 tgggccgtgt cgtcctaaag gcgctgctgc tcttcgcgct ggtcaatgcc gccttcgccg
     8101 ccgttcgtcc gctggaagcg ctcggccggc tgtcgcttta caacagcctc ttccctggcc
     8161 gcgcccgcct accctatggc gaagtcatcg ccgccgacta taacctgacg cttaacaacc
     8221 tcccggccat gttcgccgcc catgcgttgg cccggccaaa ggatgccgac gagtaccgcg
     8281 tcgtcctcat cggtgattca ggcacgtggg gctggttcct ggccaacgac gacacgctg
//
LOCUS       DAOSMQ010000075         8304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8549_length_8304_cov_4.380652, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000075 DAOSMQ010000000
VERSION     DAOSMQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8549_length_8304_cov_4.380652"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1055)
                     /locus_tag="PK829_03885"
     CDS             complement(78..1055)
                     /locus_tag="PK829_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOU40391.1"
                     /translation="MGTFFRNQRNFTRFAWFTLAFLVLVVLWGAFVRATGSGAGCGSH
                     WPLCNGVIIPRAARVETLIEFAHRISSGLSGLLVLGMWLWAFRLYPRGHVVRKGATLA
                     LVFIITESLLGAGLVLFEWVAYNASVARAISMALHLINTYLLLGAVTLTAWWATQTLA
                     GRDTTLRLRGQGKVGWLVGGGLLLLLFLGVSGAVTALGDTLFPAGTLSEGISQDFLPT
                     AHFLVRLRVYHPLVAIVTGIYLALASRAIAAERPGEATRRLARVLLVVVAVQLVAGFV
                     NVRLLVPVWMQIIHLLLADVMWIAALLLSVTVLSQEAAVAPQWLPQVGD"
     gene            complement(1213..3456)
                     /locus_tag="PK829_03890"
     CDS             complement(1213..3456)
                     /locus_tag="PK829_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOU40392.1"
                     /translation="MARAEPPADKPAKPRADETPPPDPRSWDERLIDGLAVRRREVGG
                     VLLFLLAIITLLALAGLTQAGWLNWWTRLLRQIFGWGAYGVAVFMAVLGVYVTLGHER
                     LPRLPAAGRVAGLVVLIVTLMAASHLLGGYSLADAFAGRGGGVIGWALSEPLFDFLGG
                     FLSALVYLALLFWAAMLLAGRTADDLRHWLERAAAGLADWADRVDPERIDVVGHAELV
                     QWGGAQAQPAATPPAAPARGPVIVEPDGEAGPHRRDPRLPALALLERGETASQSRDEV
                     EWKSHRIEETLQDFGLPARVVEVRRGPAVTQFGVEPGYVERPGPDGEMKQQKVRVGQI
                     AALQRDLTLALAVSRLRIEAPVPGRSVVGIEVPNGETSLVRLRNILETRDFARLNSSL
                     GVALGRDVAGAPVTTDLAKLPHLLIAGTTGSGKSVCINAFIACLVFNNTPEQLKLVMI
                     DPKKVELVRFNGLPHLIGRVEVDADRAVGVLRWLTAEMDRRYELFETTSARNIGGYNR
                     KAGRGQRLPYLAVFIDELADLMHMYPGDVERNLCRLAQMARATGIHLVVATQRPSTDV
                     ITGLIKANFPARLSFVVASGTDSRVILDTVGAENLLGRGDMLYQAPDAGAPARVQGVL
                     VTDTEIERLVEHWRTTMPDHQPEDPPWEPLIARYALLDETDSLLESAVELAKKHDQLS
                     VSFLQRRLRLGYPRAARLMEHLYEMGLVDDPQTGGKTRRSFVGEEDDDPIGKYLSDQD
                     DQNNQDE"
     gene            complement(3469..4413)
                     /locus_tag="PK829_03895"
     CDS             complement(3469..4413)
                     /locus_tag="PK829_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU40393.1"
                     /translation="MEESFGADEPAPVGAADAVEAEPEAEAAAETAPPHELIFPAPPP
                     GDPWQMAQEMMAADRTLHLRVVGYNKGGLLVFWNGIQGFVPASQLIDFPQFHVSRERL
                     QALAAWQDRELTLKIIEVNRGNSRLIFSERATLVAAEQREVLLLGVRQGERRRGLVTN
                     LTDFGAFVDLGGLEGLIHISEISWSRVTHPSTLLRPGQEVEVLVLSVDKAAERVALSI
                     KRLHVDPWQDVESRYRPDQLVTGVVGNVTTYGAFVILEEELEGLIHISELAEGRFLHP
                     RDVVRSGETVTARVLSVDGRHKRLALSLRGVDPAAPQP"
     gene            4717..5433
                     /locus_tag="PK829_03900"
     CDS             4717..5433
                     /locus_tag="PK829_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40394.1"
                     /translation="MKTQTALFPAVLPALVADAAGLASLFLLFPGLSDRLGEPGGINA
                     LLFAGGFLLFCLGVFWLRRLRPVAGGGEWASRGWRVGLAAVFAVAMSLAIAWQLGFFR
                     SGLEVDTRTLGEGGSAAYFVFGPGAWLVFSALYVLVLGFTVRPSVDPAGGRWTTVATL
                     ALLATNVMLLLASAQAHHMVGEAALWLPLVFALLLVLFLPPRLVYIARGPGFPSPAGY
                     VVLGLFLMLVGAYALAIIPI"
     gene            complement(5420..6040)
                     /locus_tag="PK829_03905"
     CDS             complement(5420..6040)
                     /locus_tag="PK829_03905"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU40395.1"
                     /translation="MTLVWQLLWAALAFWLGALPFAVWVGRRAARRDIREVGDHNPGA
                     TNVLRASGFGPFLLVMSLDISKAAAPVGLAYHIFGWRGWPMALIAIAPPLGHAFSPFL
                     RGRGGKAVAAVFGVWIGLTIWELPVVALAGIAFWFALLTVSGWVVVLSLLGMLVYLLL
                     WRPDPLLIAVAVAQLPLLVYTHRADLTRRPGFRPASRFRPPAARSG"
     gene            complement(6037..7032)
                     /locus_tag="PK829_03910"
     CDS             complement(6037..7032)
                     /locus_tag="PK829_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HOU40396.1"
                     /translation="MYRFVRNTMHPARYHGRLRDQQPPYFEGWYFRIVDATEAHRFAI
                     IPGIIWSGRPHAFVQVLDAAGGAYYHEYPVDAFWTPDDRFEVHAGPNRFNHNEMTLLI
                     DRPEQSVAGTVRFGGLTPWPVTWAAPGIMGWYAWVPFMECYHGVVSLDHAIYGALTVN
                     GQEIDFTGGRGYIEKDWGRAFPEAWIWFQTNLFQEPGTCITASIAIIPWLMTAFPGFI
                     IGLWHDGQLYRFATYTGAKTERLHVTEHTIEWVVASKKYWLEMFVTQGAGSTFGLLKG
                     PDRAEMGVRVVETLSATVHVRLTERIGSRVVYEGDGRHAGLEVHEVEARLLKMVR"
     gene            complement(7039..7497)
                     /locus_tag="PK829_03915"
     CDS             complement(7039..7497)
                     /locus_tag="PK829_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40397.1"
                     /translation="MDLLELLQRDGEKIVTEAGEALARSSLAHYAEAGQAIGQERLGR
                     LYELTVESIADRNLVPVMDYMAAVANDRFHAGYAIREVQIAINVLEESIWNDIVANVP
                     PDELARALGLVATVLGAAKDALARAYVSLASRKKAPSLDLSALFEGSTGG"
     gene            complement(7503..7919)
                     /locus_tag="PK829_03920"
     CDS             complement(7503..7919)
                     /locus_tag="PK829_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU40398.1"
                     /translation="MDAIITLQDEMHFTALPTSGQLVHMDSKPSETGLRQGASPMELL
                     LSALGGCTAMDVISILRKKRQAVTGFEVRVHGERTTEHPQVFTDITIEYVVRGVNIDP
                     AAVARSIELSTETYCSVHAMLHQAANIRTAYTIVQE"
     gene            complement(7981..>8304)
                     /locus_tag="PK829_03925"
     CDS             complement(7981..>8304)
                     /locus_tag="PK829_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HOU40399.1"
                     /translation="DHAVRLVTVPVGYGDGYFRALSNRAEVIIRGRRYPVVGRVCMDQ
                     LMVNIEWDSAYNGDEVILLGEDAGGAAVRVEELAEWAGTVPWEILTNINTRVPRVYRN
                     AAPPA"
BASE COUNT         1405 a         2755 c         2830 g         1314 t
ORIGIN      
        1 gcccatcgtg ccgatgtacc agtcggtcat gcgctcctca ttgcggcggc gcggcacatc
       61 gggccgagca ccgccgctca gtcgcctact tgcggcagcc actgcggcgc gacggccgct
      121 tcctggctca gcaccgtcac gctcaacagg agcgcggcga tccacatcac gtcggctagc
      181 agcaggtgga tgatctgcat ccacaccggg accagcaggc ggacgttgac gaagccggcc
      241 accagctgca ccgcgacgac gacgagcagc acgcgggcca gccggcgcgt cgcctcgccc
      301 ggccgctcgg cggcaatggc gcggctggct agagccagat agatgcccgt gacaatggcg
      361 acgagcgggt gatagacgcg caaacgcacc aggaagtggg ccgtcggcag gaagtcctgg
      421 ctgatgccct cgctaagggt gccggccggg aagagcgtgt cgcccagcgc cgtcaccgcg
      481 ccgctgacgc ccaggaagag caacagtaac agcccgccgc caaccagcca gccaaccttg
      541 ccctggccac gcaggcgcag agtggtgtcg cgcccggcca acgtctgcgt cgcccaccac
      601 gccgtcagcg tcaccgcgcc cagcagcagg taggtgttga tcaggtgcag agccatggag
      661 atggcccgcg ccaccgaggc attgtaggcc acccactcga acagcaccag gcccgcgccc
      721 agcaggctct cggtgatgat gaacaccagc gccagcgtcg cccctttgcg aacgacgtgg
      781 cccctggggt agagacggaa ggcccacagc cacataccca ggacaagcag gcccgacaag
      841 ccgctgctga tgcggtgggc gaactcgatc agcgtctcca cgcgcgcggc gcgggggata
      901 atcacgccgt tgcacagcgg ccaatggctg ccgcagcccg cgccggagcc agtggcgcgc
      961 acgaaggcgc cccacaggac gacaagcacc aggaacgcca gggtgaacca ggcgaaacgg
     1021 gtaaagttgc gttgattgcg gaagaatgta cccatagggc cgattatagc ccggcgcgcc
     1081 gttgtcctca attggaatgc ggggctttcc caaggtcgtc tggcgaccat tgacctgaca
     1141 ggtcggtgcg ccgggggtga ctttggcaaa gcccccaatt ggaatacggc cggggcgcgg
     1201 gtcgtcgcgc ccctattcgt cctggttgtt ctggtcgtcc tggtcggaga gatacttgcc
     1261 gatggggtcg tcgtcctctt cgcccacgaa gctgcgccgg gtcttgccgc cggtctgcgg
     1321 gtcgtctacc aggcccatct cgtagaggtg ctccatcagc cgcgccgcgc ggggatagcc
     1381 gagccgcagg cggcgctgca agaacgacac tgacagctgg tcgtgcttct tggccagttc
     1441 tacggccgac tccagcaggc tgtcggtctc gtccagcagg gcgtagcggg cgatgagcgg
     1501 ttcccagggc gggtcttccg gctgatggtc gggcattgtc gtccgccaat gctccaccag
     1561 ccgctcgatc tccgtgtccg tcaccaggac gccctggacg cgcgccggcg cgccggcgtc
     1621 gggggcctga tagagcatgt cgccgcggcc gagcaagttc tccgcgccca cagtgtcgag
     1681 gatgacgcgc gagtctgtgc cggaggcgac aacgaacgac agccgggcgg ggaagttggc
     1741 cttgatgagg ccggtgatga cgtcggtcga agggcgctgg gtggccacga cgaggtgaat
     1801 gcccgtggcc cgcgccatct gcgccaggcg gcagagattg cgctcgacgt cgccggggta
     1861 catgtgcatc aggtcggcca gctcgtcgat gaaaacagcc aggtagggca gccgctggcc
     1921 gcggccggcc ttgcggttgt agccgccaat gttgcgcgcg ctagtcgtct cgaataactc
     1981 atagcggcgg tccatctccg ccgtcagcca gcgcaggacg cccacagccc ggtcggcatc
     2041 cacctcgacg cggccgatga gatgtggcag gccgttgaag cgcaccaact cgaccttctt
     2101 ggggtcgatc atcaccagct tcagttgctc cggggtgttg ttgaagacca gacaggcgat
     2161 aaaggcgttg atgcacaccg acttgccgga gccggtcgtg ccggcgatga gcaggtgggg
     2221 cagcttggcc aggtcggttg tcaccggcgc gccggccacg tcgcggccga gggccacgcc
     2281 cagcgaggag ttgagccggg cgaagtcgcg cgtttcgagg atgttgcgca ggcgcacgag
     2341 actggtttcg ccgttgggca cctcgatgcc gacgacgctg cggccgggga cgggcgcttc
     2401 gatgcgcagg cgcgagacgg ccagggccag ggtcagatcg cgctgcaggg cggcgatctg
     2461 gcccacgcgc actttctgct gcttcatctc gccgtctggc cccggccgct cgacgtagcc
     2521 cggctcgacg ccgaactgcg tcaccgccgg gccgcgccgc acttcgacca cccgcgccgg
     2581 caggccgaag tcttgcaacg tttcctcgat gcggtggctc ttccactcga cctcatcgcg
     2641 cgattgggac gcggtctcgc cacgctccag cagggccagc gcgggcagcc gtgggtcgcg
     2701 gcggtgcggg ccggcctcgc cgtccggctc cacgatcacc ggcccgcgag caggcgcggc
     2761 cgggggtgtc gccgccggtt gggcctgcgc cccgccccat tggaccaact cagcgtggcc
     2821 gacgacatcg atgcgctccg ggtctacacg gtcggcccag tcggccagcc cggccgcggc
     2881 tcgctccagc cagtggcgca gatcgtcggc cgtgcgcccg gccagcaaca tcgccgccca
     2941 gaaaagcagg gccagataga ccagagccga gaggaagccg ccgaggaagt cgaacagcgg
     3001 ctcggacagc gcccagccga tcaccccgcc gccgcggccg gcaaaggcgt cggccaggct
     3061 atagccgccc aggaggtggc tggcggccat aagcgtgacg atcaggacga ccagcccggc
     3121 gacgcgcccg gcggccggca gccggggcaa gcgctcatgg ccgagagtga cgtaaacgcc
     3181 cagcacggcc atgaacacag ccaccccgta agcgccccag ccgaagatct gtcgcagcag
     3241 ccgcgtccac cagttcagcc aaccggcctg cgtcaggccg gccagggcca gaagagtgat
     3301 aatcgccagc aggaaaagaa ggacgccgcc aacctcgcgg cggcgaacag ccaggccatc
     3361 gatcagccgc tcatcccacg agcgaggatc gggcgggggg gtctcgtcgg cgcggggctt
     3421 ggccggcttg tcggcagggg gttccgcgcg agccataggc ggctccggct acggctgcgg
     3481 cgcggcgggg tcaacgccac gcaacgacag ggccagccgc ttgtggcggc cgtcgacgct
     3541 caggacgcgc gccgtcaccg tctcgcccga acgtaccacg tcgcgcgggt gcaggaagcg
     3601 cccctcggcc aactcggaga tgtggatcag gccttccagc tcctcctcca ggatgacgaa
     3661 cgcgccgtag gtagtcacat tgccgacaac gccggtcacc aactggtcag ggcgatagcg
     3721 gctctcgacg tcttgccagg ggtcaacgtg caggcgcttt atgctcaggg cgacgcgctc
     3781 ggcggccttg tcaacgctaa gcactaatac ctcaacctcc tggcccggcc gcaggagggt
     3841 gctggggtgc gtcacgcggc tccaggagat ttcggagatg tggatgaggc cttccaggcc
     3901 gcccagatcg acaaaagcac cgaagtcggt caggttcgtg acaaggccgc gccgccgctc
     3961 gccctgcctg acgcccaaca gcagtacctc gcgctgttcg gcggccacca acgtcgcccg
     4021 ctcggagaag atgagccggc tgttgccgcg gttgacctcg atgatcttga gggtcagctc
     4081 gcggtcttgc cacgccgcca acgcctgtag ccgctcgcgc gacacatgga actggggaaa
     4141 gtcgatcaac tgcgaggcgg gcacgaaacc ctgaatgccg ttccagaaga cgagcagacc
     4201 gcccttgtta tagccgacaa cgcgcagatg gagcgtgcga tccgcggcca tcatctcctg
     4261 agccatctgc cacgggtcgc ctggcggcgg ggcagggaaa atgagttcgt gtggcggcgc
     4321 agtctcagcg gctgcttcgg cttctggctc cgcctcgaca gcatcggcgg caccgaccgg
     4381 cgccggctcg tcggcgccga acgactcttc cattcgaaat aaggccgtcc agtaaccatc
     4441 atctacttgt gggccggcct gcgggtccct tgcttgctcc tgactcatac tacccaaccg
     4501 ttacaaccct gaaaagcgcc cacggccgct tcccaacgcc gcgagtcact gacggacaac
     4561 gtcgccagtg ggtgaacaga acgaagtcgc cagacccagc agtacaaccc acgacggtaa
     4621 gctgaattat agggcttgag tggcgactgt caaccagtaa tacaccgttc gtgacggatt
     4681 tcacgagtgg ccccacactc tgctataatg ccggggatga agacccaaac ggcgctattt
     4741 ccggccgtcc tgcccgcgct ggtggctgat gccgccgggc tggcgtccct gttccttctg
     4801 ttccccggcc tcagcgacag gctgggcgaa cctggcggca tcaatgcgct gctgtttgcc
     4861 ggaggctttc tgctgttctg cctcggcgtg ttctggctgc ggcgtctgcg gccggtggcc
     4921 ggcggcggcg aatgggcctc ccgcggctgg cgcgtcgggc tggcggccgt ctttgccgtg
     4981 gccatgagcc tggccatcgc ctggcagctg ggtttctttc gatctgggtt agaggtggat
     5041 acgcgcacgc tgggcgaagg cggttcggcg gcgtacttcg tcttcggtcc gggcgcgtgg
     5101 ctggtctttt cggcgctcta tgtcctggtg ctgggcttca cagtgaggcc aagcgttgac
     5161 ccggccggcg gacggtggac gaccgtagcc acgctggcct tgctggccac caacgtcatg
     5221 ctccttctgg ccagcgccca ggcgcaccat atggtgggtg aggcggcgct gtggctgcca
     5281 ctcgtgtttg ccctgctgct ggtgctcttc ctgccgccgc ggctggtcta tatcgcccgc
     5341 ggccccggct ttccctcgcc ggcgggctac gtagtgctgg ggctgttcct catgttggtt
     5401 ggcgcgtacg cgctggcgat catcccgatc tagccgcggg cgggcgaaag cgcgacgccg
     5461 gtcggaagcc aggccggcga gttaggtcgg cgcgatgggt atagaccagc agcggcagct
     5521 gggcgacggc cacggcgatc agcagtggat cgggtcgcca caggagcaga tagaccagca
     5581 ttcccagcag tgacagcacc accacccagc ccgacaccgt tagcagcgcg aaccagaaag
     5641 caatgcccgc cagagccacg acgggcaact cccagatcgt cagcccaatc cacacgccaa
     5701 agacggccgc gacggccttg ccgccccggc cgcgcaggaa gggcgagaag gcgtggccca
     5761 gcggcggggc gatagcgatg agcgccatcg gccaaccacg ccagccgaag atgtgatagg
     5821 ccagccccac cggcgcggcg gccttagaga tgtccaggct catgaccagc aggaacgggc
     5881 cgaagccgct ggcccgcagc acgttggtcg cgccggggtt gtggtcgccc acttcgcgga
     5941 tgtcgcggcg ggcggcgcgg cggccgaccc agacggcgaa cggcaacgcc cccagccaga
     6001 aggccagcgc ggcccagagc agttgccaga cgagcgtcat cgcaccatct tcagtagccg
     6061 cgcctcgacc tcgtggactt ccaggccggc gtggcggccg tcgccctcat agacgacccg
     6121 gctgccgatg cgctcggtca ggcgcacgtg gacggtggcc gacagcgtct cgaccacgcg
     6181 cacgcccatc tcggcccggt ctggcccctt cagcaaacca aaggtgctgc cagcgccctg
     6241 ggtgacgaac atctccagcc agtacttttt gctggctaca acccactcaa tggtatgctc
     6301 ggtgacgtgc agccgctccg tcttcgcgcc ggtgtaggtg gcgaagcgat agagctggcc
     6361 atcgtgccac aggccaatga tgaagccggg gaaagcggtc atcagccagg ggatgatggc
     6421 gatggaggcg gtgatgcacg tgcccggctc ctggaaaagg ttggtctgga accagatcca
     6481 cgcttcgggg aaggcgcggc cccagtcctt ttcgatgtag ccacgcccac cggtgaagtc
     6541 gatctcctgg ccgttgaccg tcagcgcgcc gtagatggca tgatcgagac tgacgacgcc
     6601 gtggtagcac tccatgaagg gtacccaggc gtaccagccc ataatgcccg gcgcggccca
     6661 ggtgaccggc cagggcgtca gcccgccaaa gcgcaccgtc ccggcgaccg actgctccgg
     6721 ccggtcgatg agcagcgtca tctcgttgtg gttgaaacgg ttggggccgg cgtgaacctc
     6781 gaagcggtcg tcgggcgtcc agaaggcatc gaccgggtac tcgtggtaat acgcgccgcc
     6841 ggcggcgtcg agcacctgga cgaaggcgtg gggccggccg ctccagatga tgccggggat
     6901 gatggcgaag cggtgcgcct cagtggcatc gacgatgcgg aagtaccagc cctcgaagta
     6961 cggcggctgt tggtcgcgca accggccgtg gtagcgcgcc gggtgcatcg tgttgcgtac
     7021 aaagcggtac acgtgggctt agccgccggt ggagccttcg aatagagccg acaagtcgag
     7081 cgaaggcgct ttcttgcggc tggccaatga gacgtaggcg cgggccagcg catccttggc
     7141 cgcgcccaac acagtcgcca ccaggcccag cgcccgcgcc agttcatccg gcggcacgtt
     7201 ggcgacgatg tcgttccaga tggactcttc gagcacgttg atggcgattt gtacttcacg
     7261 gatagcgtag ccggcgtgga agcggtcgtt ggccacggcg gccatgtagt ccatcaccgg
     7321 cacgaggttg cggtcggcga tgctctcgac agtcagctca tagagccggc cgagtcgctc
     7381 ctggccgatg gcttgccccg cctcggcata gtgggccagg ctggagcgag ccagcgcctc
     7441 gccggcctcg gtgacgatct tctcgccgtc gcgttgcagt agttctagca ggtccatacc
     7501 gtctattcct gaacgatggt gtaggctgtg cggatgttgg cggcctgatg cagcatggca
     7561 tgaacggagc agtaggtctc ggtggacagt tcgatgctcc gggccacggc ggccgggtcg
     7621 atgttgacgc cgcggacgac gtactcaatg gtgatgtcgg tgaagacctg ggggtgttct
     7681 gtcgtccgct cgccgtggac gcgcacttcg aagccggtca ccgcctgccg cttcttacgc
     7741 aggatggaga tgacatccat cgccgtgcag ccgcccaggg cgctcagcag cagttccatt
     7801 gggctggccc cctggcgcag tcccgtctcc gacggcttgc tgtccatgtg gacaagctgg
     7861 ccgctggttg gcaaagcagt gaagtgcatc tcgtcttgca gggtaatgat cgcgtccatg
     7921 aacagtaggc tcccagaagg tgatgtgttg ggctgaattt cccatattcc ggtggagccg
     7981 tcaagcgggc ggcgccgcgt tccggtagac gcgcggcacg cgcgtattga tgttggtcag
     8041 gatttcccac ggcacggtgc cggcccactc ggccagttct tcgacgcgca cggccgcgcc
     8101 gccggcgtcc tcgcccagca agatgacctc gtcaccgttg taggccgagt cccattcgat
     8161 attgaccatg agctggtcca tgcagacgcg gccgacgacc gggtagcgcc gcccgcggat
     8221 gatgacttcg gcgcggttgg acagggcgcg gaagtagccg tcgccgtagc ccacgggcac
     8281 ggtgacaagg cgcacggcgt ggtc
//
LOCUS       DAOSMQ010000076         8303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8553_length_8303_cov_4.840810, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000076 DAOSMQ010000000
VERSION     DAOSMQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8553_length_8303_cov_4.840810"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1903
                     /locus_tag="PK829_03930"
     CDS             35..1903
                     /locus_tag="PK829_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOU40400.1"
                     /translation="MTLRPSPTLARRLLIAAPVLLAALALLLWPAAGQAQDPVLPAAT
                     PDGFPGLELFAGRCANCHGETGAGDGELILQAGNQPPMPFDAAYIQQAMPSVMFDQIT
                     NGEAAVGMPPFGPASSNPIDDAGRWNLVAAVYSLATSPENVAAGQEVYAANCAACHGD
                     AGQGDGPDAATTDPAAGPLTDVAYWFNRSNDTVVADLAPGVVPAHAYDLPATDLQRVV
                     DFARTFSYVYADPAVLNEPIAAGTITGVLTNGSSGQALANTAVTLRAFTAELEETLTL
                     TTTTGTDGAFQFDVTDTPPGWVYIVNAAYDGLSFNSGADQLSRTRTALDMPVTVFDKT
                     TDATAISIAQLHVVMEFAEDLIQVSQLYIFSNAAQAVYVGPTGNPAEGVVEVAVPEGA
                     QNLTFQRSFGAMDSFLAANDFVATARGWADPLPLRPGETSLTLLVRYALPYVDGLTIT
                     HPVFYNTASGAIILPDNGVDVVGADWVEGAPQTFGEGEVFRNFGRAGLAAGDALTIQL
                     EGRPSVINDATTGQAVANRDTTSELLIGGGALLLAAVGGIFLWRYWQGRQEESAYEEA
                     PAYAAPAAAPGLGQRDDLLRRIAALDDAFENGRLQEADYRAQREELKQQLAAVWGR"
     gene            2054..2374
                     /locus_tag="PK829_03935"
     CDS             2054..2374
                     /locus_tag="PK829_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HOU40401.1"
                     /translation="MHKFTVLYYRVDDETALEEFFSGTHLPLLESLPGLRAVEVSRVL
                     GQPLGQSRFTLTVEAMFDSRAALEAALSTAAGMTLMNALRPWAEARLITWYYADSFST
                     DAAP"
     gene            2563..3036
                     /locus_tag="PK829_03940"
     CDS             2563..3036
                     /locus_tag="PK829_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HOU40402.1"
                     /translation="MKDVYGLHLMMRVANVRDRAALGDGETIDRFLVDLVHELGMNVL
                     AGPMVTEEAGEPQRAGYSGVIILYESHAAIHTYSTLGQAFVDVFSCKPYDTSRVEAVM
                     SRHFGAFEVVEQTMLGRGVHWGSNVEREMATWSAARDGEAARDDATKPQAAGSFG"
     gene            3116..4069
                     /gene="speB"
                     /locus_tag="PK829_03945"
     CDS             3116..4069
                     /gene="speB"
                     /locus_tag="PK829_03945"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOU40403.1"
                     /translation="MAHPVNPALGGHLLAYSGVPTFMRQTPTRDLSGIDVAIVGVPFD
                     SGATSFRGGARFGPRKIREASLALWGHHPIHGVAPAEALAIADYGDVAIEMASIDKTM
                     ANITAEVGAVLAQGPMVVALGGDHSISLPLLRAQAAAARNGGHAGPLALVHFDAHTDV
                     EPGDYEHGTVFRHAVEEGLIDPAATIQVGIRGSLYFPDDLHVARQLGIRVLTIDECFE
                     MGIPAVVAAIRETVGARRTYVTLDIDAADPAFAPGTGTPEPGGFSSYQMLQLMRGLKG
                     LNLVGMDLVEVSPPYDHADITALLAANLVFEYLCLIALNRA"
     gene            4199..5176
                     /locus_tag="PK829_03950"
     CDS             4199..5176
                     /locus_tag="PK829_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOU40404.1"
                     /translation="MTDGPISRFLRHHYRHFNAATVIDAADGYKRHLDAGGAMLVTLA
                     GAMSTAELGLSLAEMIRRDKVQAISCTGANLEEDLFNLVAHDFYERVPHYRDLTPQDE
                     LALLGRHMNRVTDTCIPEEEAMRRLETALVDEWTRADQAGERYFPHEFMYRVLRSGVL
                     AQHYQIDPADSWMLAAAEKNLPMVVPGWEDSTMGNMYAAHVMSGDIDNVHTVRTGIEY
                     MAYLANWYAAVAPTRSIGFFQIGGGIAGDFPICVVPMLHQDLGQTDVPLWGYFCQIGD
                     STTSYGSYSGAVPNEKITWGKLGVDTPKYMIESDATIVAPLIFALVMEE"
     gene            complement(5269..6408)
                     /locus_tag="PK829_03955"
     CDS             complement(5269..6408)
                     /locus_tag="PK829_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU40405.1"
                     /translation="MKKVLLIGVIALLAVLAACGGGPSGGPGSAGAEPTAAASANGEV
                     QLDDELSVYNWEDYIDEQILRDYEERYGVRIVYDTYASNEDLLTKLQAGAAGYDVIFP
                     SDYMVTQMIELGLLAEIDVSAMPNFANIGAEFKDPPYDPGNRFCTPYQWGMTGIAYRN
                     GRFEEPPDSWAYLFDPAKLAELAPEGINVLNDQRELLSAALFYLGYSPNTTERAELEA
                     ARDLILAAKPYWKTFNSEDYYSTLMVSDEIVLSHAWSGDAAQAFWATYDDETGEGNWL
                     FTVPKEGAVRYQDNVCIPASSRRQATAQHFMNYLMEPEVAAAITNFTFYPSPNEAAKE
                     FIDPDILNNPGIYPPAEVRAKMQWLTDVGDAVFIYDEMWTAIKGQ"
     gene            complement(6567..7430)
                     /locus_tag="PK829_03960"
     CDS             complement(6567..7430)
                     /locus_tag="PK829_03960"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40406.1"
                     /translation="MTATTPAPSTTPAAGARTQALTAPRPARRDVRRSFNSNALTLLA
                     AAIFLFLYLPILILVIYSFNANSVVGVWTGVSTRWYAELLHDRALIASFRVSMWVAFW
                     STLISTVLGTLGAMALERFRFRGKLTFDATMYLPIIIPDIVMALSTLLFFVLVAVPLS
                     RYTILVAHVAFNISFVAVVVRGRLAEMDRGLEEAAADLGASAWTTFRRVTLPLLTPAI
                     VSAALLAFTLSLDDFVITFFVAGPGSTTLPVRVYSMIKFGVTPEVNAISTLMLLGSTV
                     LVVVSLWLQRK"
     gene            complement(7427..>8303)
                     /locus_tag="PK829_03965"
     CDS             complement(7427..>8303)
                     /locus_tag="PK829_03965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40407.1"
                     /translation="LAIVFFVSLGERTRLGTVTYPPLSLGSLGTLFDDYARVFSRVGG
                     DFIYLRIFGRSAWLALLNTALCLLFGFPLAYWIARQPANRRNMWIFLVMIPFWTNFLV
                     RTYAWMLILRDSGLINSAWTGSFHDAATVIAGHAPALGWLADITARPLPLLYNVGAVL
                     VGLFYGYLPFMVLPLYSNLEKTNWSLLEAAADLGDNGFQRFTRVLLPLSLPGIIAGSI
                     IVFIPSLGAYVTPDLMGGAKVSLLGNLLQQQFMTVRDWPFGSALSFIMMAVMLAATLV
                     YFRASAEAERRQEKS"
BASE COUNT         1492 a         2825 c         2668 g         1318 t
ORIGIN      
        1 gcccaggccg ctcgcgcctg atagggatag acccatgacc cttcgccctt ctccgaccct
       61 tgcgcgccgg ctactcatcg ccgcccccgt cctgctggca gcgctggctt tgctgctttg
      121 gccagcggcc gggcaggccc aggaccccgt gctgcccgcg gccacgcccg acggcttccc
      181 cggcctggag ctattcgccg gccgctgcgc caactgccac ggcgagaccg gcgcgggcga
      241 cggcgagctg atcctgcaag ccggcaacca gccgcccatg cccttcgacg ccgcctacat
      301 ccagcaggcc atgccctccg tcatgttcga ccagatcacc aacggcgagg cggccgtggg
      361 catgcctccc ttcggcccgg ccagcagcaa ccccatcgac gatgccggcc gttggaacct
      421 ggtcgccgcc gtctatagcc tggccacgtc gccggaaaat gtggccgctg gccaggaagt
      481 ctacgccgcc aactgcgccg cctgccacgg cgacgccggc cagggcgacg gccccgacgc
      541 ggctaccacc gaccccgctg ccggcccgct aaccgacgtc gcctactggt tcaaccgcag
      601 caacgacacc gttgtagccg acctggcccc cggcgttgtg cctgcccatg cctacgacct
      661 gccggcgacc gacttgcagc gggtcgtcga tttcgcgcgc accttcagct acgtctatgc
      721 cgatccggcc gtgctcaatg agccgatcgc cgccgggacg atcaccggcg tgctgaccaa
      781 cggcagctcc ggccaggcgc tggccaacac cgcagtgaca ctgcgagcct tcaccgccga
      841 gctagaagag acgctcacgc tgacgacgac gaccggcacc gatggcgcgt tccagttcga
      901 cgtgaccgac acgccgcccg gctgggtcta tatcgtcaac gccgcctacg acggtctgag
      961 cttcaacagc ggcgccgacc agctgagccg tacccgcacg gcgctagata tgcccgtcac
     1021 cgtcttcgac aagacgaccg acgccaccgc catcagcatc gcccagctgc acgtggtcat
     1081 ggagttcgcc gaagacctga ttcaggtcag ccaactctac atcttcagca acgccgcgca
     1141 ggccgtctat gtggggccga cgggcaaccc ggcggagggc gtcgtcgagg tggccgtgcc
     1201 cgaaggggcg cagaacctga cctttcagcg ctcctttggg gcaatggatt ccttcctggc
     1261 cgctaacgac tttgtggcca ccgcgcgcgg ctgggccgac ccgctgccgc tgcgccccgg
     1321 cgaaacctca ttgaccctgc tggtgcgcta tgcgctgccc tacgtcgacg gcctgaccat
     1381 cacgcacccg gtgttctaca acactgcctc cggagctatc atcttgcccg acaacggcgt
     1441 ggatgtcgtt ggcgcggact gggttgaggg agcgccgcag acattcggcg agggcgaagt
     1501 ctttcgcaac ttcggccgcg ccgggctggc cgcaggcgac gcgctgacta tccagctgga
     1561 gggccggccg tcagtgatca acgacgccac caccggccag gccgtggcca accgcgacac
     1621 aaccagcgaa ctgctcatcg gcggtggagc gctgttgctg gccgcggtcg gcggcatctt
     1681 cctgtggcgc tactggcagg ggcgacagga ggagagcgcc tatgaagaag ctcccgccta
     1741 tgccgcgccg gcggccgccc caggcctggg ccaacgcgac gatctgttgc gccgcatcgc
     1801 cgccctggac gacgccttcg agaacggccg gttgcaagaa gccgactacc gcgcccagcg
     1861 cgaggaactg aagcagcagc tcgccgcggt gtgggggaga tagagtgggc agtaaacagt
     1921 aaacagtagg tcagtagaga ccgatcgctg acgacagacg gctgaccacc gacctattga
     1981 ccactggaca ctggacactg acccactgaa cactggatac tgaccactga ccactgctca
     2041 cggaccacta accatgcaca aattcaccgt cctctactac cgcgttgacg acgagacggc
     2101 gctggaggag ttcttcagcg gcacgcacct gccgttgctg gagagcctgc cggggctgcg
     2161 cgctgtcgag gtcagccgcg tgctgggcca gccgctgggt cagtcgcgct tcacgctgac
     2221 cgtcgaggcc atgttcgaca gcagggcggc gctggaagcg gccctgagca ccgcggccgg
     2281 gatgacgctg atgaacgccc tgcggccgtg ggccgaggcg cgcctgatca cctggtacta
     2341 cgccgattcc ttcagcaccg acgccgcccc ctgatccatc ccccatcccc cacccccctc
     2401 ctaatgggga caaaacatta gaatatcgca ggttgcccca ctaatgggtt tacattggcc
     2461 ccgttttgtg cataatacag actgtccacg cgacgcaagg aaccttcccc ccaggttagc
     2521 ggacagatta atattccact cttccgagga gagatgtttc ccatgaaaga cgtgtatggt
     2581 ctgcatctga tgatgcgcgt cgccaacgtg cgcgaccgcg ccgcgctggg cgacggcgaa
     2641 acaattgatc gtttcctggt ggacctggtc cacgagttgg gcatgaacgt actggccggg
     2701 ccgatggtga ccgaagaggc cggcgagccg caacgcgctg gctattccgg cgtcatcatc
     2761 ctgtacgagt cccatgccgc catccacacc tatagcaccc tgggccaggc gttcgtggac
     2821 gtgttctcgt gcaaacccta tgacacctcg cgcgtcgagg cggtcatgag ccgccacttt
     2881 ggcgcgttcg aggtcgttga gcagacgatg ctcggccgcg gcgtccactg gggcagcaat
     2941 gtcgagcgcg agatggccac ctggtcggcc gcccgggacg gcgaggccgc ccgcgacgac
     3001 gcgaccaagc cgcaagcggc cggcagcttt ggctgagtgg tgggatgagc gaccgcgatg
     3061 acttcgcccc tccttccccc attcacccag tccaccaact gacaaaggaa tagctatggc
     3121 tcatcccgtg aaccccgccc tgggcggaca tctgctggct tactccggcg tgcccacctt
     3181 catgcgccag acgccgacac gcgacctgag cggcatcgac gtggccatcg ttggcgtacc
     3241 cttcgacagc ggcgcgacca gcttccgcgg cggcgcgcgc ttcggcccgc gcaagatccg
     3301 cgaggcatcg ctggccctct ggggccacca tcccatccac ggcgtcgccc cggccgaggc
     3361 gctggccatc gccgactatg gcgacgtggc catcgagatg gccagcatcg acaagacgat
     3421 ggccaacatc accgccgagg ttggcgcggt gctggctcag gggccgatgg tcgtggccct
     3481 gggcggcgac cactccatca gcctgccgct gctgcgcgcc caggcggccg ccgcgcggaa
     3541 cgggggccac gccggcccgc tggccctcgt ccacttcgac gcccacaccg acgtggagcc
     3601 gggcgactat gagcacggca cggtctttcg ccacgccgtt gaggaggggc tgatcgaccc
     3661 ggcggcgacc atccaggtcg gcatccgcgg ctcgctctac ttccccgacg atctccatgt
     3721 cgcccggcaa ctgggcatcc gcgtgctgac catcgacgag tgcttcgaga tgggcatccc
     3781 cgccgtcgtc gccgccatcc gcgagacggt cggcgcgcgc cgcacctacg tcaccctcga
     3841 tatcgacgcc gccgacccgg ctttcgcccc cggcacgggc acgccggagc cgggcggctt
     3901 cagcagctac cagatgctcc agctcatgcg cggcctgaag gggctgaacc tcgtgggcat
     3961 ggacctggtc gaagtcagcc cgccctacga ccacgccgac atcaccgccc tgctggcggc
     4021 gaatctggtg tttgagtacc tgtgtttgat cgctctgaat cgagcataag atagaagagc
     4081 tcacgcaaag gacgctaagg gagctaagga tgcaaagaag aaaaagttgt cttattcttc
     4141 ttagcgctct ttgcctcctt tgcgtccttt gcgtgagcct ctttctcaac ggagagacat
     4201 gacagacgga ccgatcagcc gcttcctgcg gcaccactac cggcacttca acgcggcgac
     4261 ggtcatcgac gccgccgacg gctacaaacg ccacctcgac gccggcgggg cgatgctcgt
     4321 cacgctggcc ggggcgatga gcacggccga gctgggcctg tcgctggccg agatgatccg
     4381 ccgcgacaag gtgcaggcca tctcctgcac cggggccaat ctggaggaag acctcttcaa
     4441 cctggtagcc cacgacttct atgagcgcgt gccccactac cgcgacctga cgccgcaaga
     4501 cgagctggcc ttgctgggcc ggcacatgaa ccgcgtcacc gacacctgca tccccgaaga
     4561 ggaggccatg cgccgcctgg agacggccct ggtggacgag tggacgcggg ccgaccaggc
     4621 cggcgaacgc tacttcccgc acgagttcat gtaccgcgtg ctgcgttccg gcgtactggc
     4681 gcagcactac cagatcgacc cggcggactc gtggatgctg gccgcggccg agaagaacct
     4741 gcccatggtt gtgcccggct gggaggactc gacgatgggc aacatgtacg ccgcccacgt
     4801 gatgagcggc gacatcgaca acgtccacac cgtgcggacg ggcatcgagt acatggccta
     4861 tctggccaac tggtacgcgg ccgtggcccc gacgcgctct atcggcttct tccagatcgg
     4921 cggcggcatt gccggcgact tccccatctg cgtcgtgccc atgctgcacc aggatttggg
     4981 ccagacggac gtaccactgt ggggctactt ctgccagatc ggcgactcga cgaccagcta
     5041 cggcagctac agcggggccg tgcccaacga gaagatcacc tggggcaagc tgggggtgga
     5101 cacgccgaaa tacatgatcg agtcagacgc gaccatcgtt gcgccgttga tctttgcgct
     5161 ggtgatggaa gagtaggggc agatcgtagg tgtaggggca ggtcgtaggg gcaggtctga
     5221 gacctgcccc tacacctacg acctgccctg cccgccggcg gcgacgacct actgcccctt
     5281 aatcgccgtc cacatctcgt catagatgaa tacggcgtcg cccacgtcgg tcagccactg
     5341 catcttggcc cgcacctcgg ccggcgggta gatgccgggg ttgttgagga tgtcggggtc
     5401 gatgaactct ttggccgcct cgttggggct ggggtagaaa gtgaagttgg tgatggccgc
     5461 ggccacttcc ggctccatca ggtagttcat gaagtgctgc gccgtggcct gccgccggct
     5521 gctggccggg atgcagacgt tatcctgata gcgcaccgcg ccctctttgg gcacggtgaa
     5581 cagccagttg ccctcacccg tctcgtcgtc ataggtggcc cagaaggcct gcgccgcgtc
     5641 gccgctccag gcgtgggaga ggacgatctc gtcgctgacc atcagcgtgc tgtagtagtc
     5701 ctcgctgttg aacgtcttcc agtagggctt ggccgccagg ataaggtcgc gggcggcttc
     5761 caattcggcg cgctcggtcg tgttgggcga atagcccagg tagaacagcg ccgccgacag
     5821 cagttcgcgc tggtcgttga ggacgttgat gccctcgggg gccagttccg ccagcttggc
     5881 cgggtcgaac aggtaggccc agctatcggg cggctcctcg aagcggccgt tgcggtaggc
     5941 gatgccggtc atgccccact ggtagggagt gcagaagcgg ttgccggggt cgtagggcgg
     6001 gtccttgaac tccgcgccga tgttggcgaa gttgggcatg gcgctgacgt cgatctccgc
     6061 cagcagcccc agctcgatca tctgcgtgac catgtagtcg gacgggaaga tgacatcata
     6121 gccggccgcg cccgcttgca gcttggtcag caggtcttcg ttggaggcgt aggtgtcgta
     6181 gacgatgcgc acgccgtagc gctcctcata gtcgcgcagg atttgctcat cgatgtagtc
     6241 ttcccagttg tagacactca actcgtcgtc gagctgcacc tcgccgttgg ccgacgcggc
     6301 cgctgtcggc tccgccccgg ccgatcccgg cccgccgctc ggccccccgc cgcaggccgc
     6361 cagcaccgcc agcagagcta tcacaccaat cagtagtact ttcttcatct cgatctcctt
     6421 ttcttctctg ggggaaggtt ccaggggcca ggggccaggg gccaggggcc aggggccagg
     6481 ggccaggggc cagggaaaga acgctcttcc ctggaacctg gaacctggaa cctaatccct
     6541 atcccctaat ccctatcccc taccccctac ttccgctgca accacagcga caccaccacc
     6601 aacaccgtcg aacccagcag catcagcgtc gaaatggcat tgacctccgg cgtgacgccg
     6661 aacttgatca tcgaatagac ccgcaccggc agcgtcgtcg agccgggacc ggccacgaaa
     6721 aaggtgatca cgaagtcgtc gagcgacaac gtgaaggcca gcagcgccgc cgagacgatg
     6781 gccggcgtca gcagcggcag cgtcacgcgg cggaaagtcg tccaggcact cgcgcccagg
     6841 tcggcggcgg cctcctccag cccgcggtcc atctccgcca accggccgcg cacgaccacg
     6901 gccacgaagg agatgttgaa ggccacgtgg gccaccagga tggtgtagcg gctgaggggc
     6961 acggccacca ggacgaagaa caacagcgtc gatagggcca tgacgatgtc gggaatgatg
     7021 atcggcaggt acatcgtcgc gtcgaaggtc agcttgccgc ggaagcggaa gcgctccaac
     7081 gccatcgccc ccagcgtgcc cagcaccgtg ctgatgagcg tcgaccagaa ggccacccac
     7141 atgctgacgc ggaaggaagc gatcagcgcc cggtcatgga gcaactcggc ataccagcgc
     7201 gtgctgacgc ccgtccacac gccgacgaca gagttggcgt tgaaggagta gatgaccagg
     7261 atgaggatgg gcagatagag gaacaggaag atggccgcgg ccagcagcgt cagggcgttg
     7321 ctgttaaagc tgcggcggac gtcgcggcga gcggggcgcg gcgcggtcag ggcctgcgtg
     7381 cgcgcgccgg cggcgggcgt cgtggacggt gctggcgttg tcgcggtcat gacttctcct
     7441 ggcggcgctc cgcctcggcc gacgcgcgga agtagaccag cgtcgccgcc aacatcacgg
     7501 ccatcatgat aaagctcagc gccgagccga atggccagtc gcgcacggtc ataaattgct
     7561 gctgcaacag gttgcccagc agcgacacct tggccccgcc catcaggtcg ggcgtcacat
     7621 aggcccccag cgagggaatg aagacgatga tcgacccggc gatgataccc ggcaggctga
     7681 gcggcagcag cacgcgcgtg aagcgctgga agccgttgtc ccccaggtcg gcggccgcct
     7741 ccagcagcga ccagttggtc ttctccaggt tgctatagag cggcaagacc atgaagggca
     7801 agtagccgta gaacaggccc accagcaccg cgccgacgtt gtagagtagc ggcagcggcc
     7861 gggcggtgat gtcggccagc cagcccagcg cgggcgcgtg cccggcaatc accgtggccg
     7921 cgtcgtggaa gctgcccgtc caggcgctgt tgatcagccc cgaatcgcgc aggatgagca
     7981 tccaggcgta ggtgcgcacc aggaagttgg tccagaaagg gatcatgacc aggaagatcc
     8041 acatgttgcg ccggttggcc ggctggcggg cgatccagta ggccagcggg aagccgaaca
     8101 gcaggcacag ggccgtgttc agcagcgcca gccaggccga gcggccgaag atgcgcaggt
     8161 agatgaagtc gccgccgacg cggctgaaga cgcgggcgta gtcgtcgaag agcgtcccca
     8221 gcgagccgag gcttagcggc gggtacgtga ccgtgcccag ccgcgtgcgc tcgcccaggc
     8281 tgacgaaaaa gacgatggcc agg
//
LOCUS       DAOSMQ010000077         8210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8691_length_8210_cov_5.108890, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000077 DAOSMQ010000000
VERSION     DAOSMQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8691_length_8210_cov_5.108890"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PK829_03970"
     CDS             <1..505
                     /locus_tag="PK829_03970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU40408.1"
                     /translation="RREGLAIRTVPGDMRDLSAFADATFDLIFHPVSNIFTPDVRAVW
                     RECYRVLRPGGALLMGTVNPIEYAFDRALADAEGIFQLKHSLPYSDVTDISTEEHARL
                     YGSHAPYEFSHTLEDQIGGQLEVGFVLTGFFEAHKEDDRLGRYFPNYIATRALKPYAT
                     PAAPTDD"
     gene            542..2143
                     /locus_tag="PK829_03975"
     CDS             542..2143
                     /locus_tag="PK829_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40409.1"
                     /translation="MTYQQLVTDHSRDPEQLEQAYRAAVKAGQGDAFRQAIDAGRASQ
                     PDNLLYAAWHYRLRDVAVAVKTSFVRWGWAVPLALLNGLLFWWLSDEERYSIQIAGGP
                     PGASTFLPTLLLLVAPLAAVFVLAYLAPVRRRWPLAVLIGVGLVAAAGYVLWVYPRLG
                     TRSFQEQYVTLMAMHLPLLAWAGVGLYLVAGHRDPGNRFAFLIKSLEVVIMGGLFAIA
                     GGLFTGITIALFSALGMEPSELVMRLFIAGGGGLIPVIAVAVIYNAARPPAGQSFEDG
                     LSKLLATLMRTLLPLTLVVLVVYIGFVLLNFREPFENRDVLIIYNGMLFAVIALLVGA
                     TPVSLAETSPRVANALRWAIVAVAGLALLVSVYALAAIVYRTAMDRLTPNRLAFIGWN
                     VVNIGLLALVVWAGARGRATDWLPRLHRAFAIGTVAYTVWTLAVILVVPWLFGIDQGA
                     LDTLPPAVRAVVYESPDPILLKCAGSPHIYLLDDGEKRWIDTIATFTERGYVWGDVHF
                     VPCDELHSLPDGEPIPADAGPPPQP"
     assembly_gap    2457..2556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2557..2919
                     /locus_tag="PK829_03980"
     CDS             <2557..2919
                     /locus_tag="PK829_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HOU40410.1"
                     /translation="LVWYLSNDPRLGLEARRILAEPSTSLIIPTLVLAEAKHISERKR
                     TTITFDEIMRTVASARRITIFPLDIFVVAALPTNLDIHDGLIVATALAARDLFAEEVA
                     VLTRDQLISASALVPVIW"
     gene            complement(2993..4582)
                     /locus_tag="PK829_03985"
     CDS             complement(2993..4582)
                     /locus_tag="PK829_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40411.1"
                     /translation="MNAATGQSATPRAWGDEAQRWRRFYADRLPGQLRLAVDFVDGRP
                     AAEVRPHFDSCLALLNAAGQRAELRPLWLALVDRLHPLPVRWGEWSLWLATLQQAADH
                     AAALGRPAQQAEYLAHAAGLLLNTGRLEPARDTAGEALRLAQESGAAWPLCLAGSVVA
                     ATLRAQARYGEAQTFVDELRADLARLPAAQPPARAAMGTARLDLETMDTLRHSAQPEL
                     ALTLSETVIDRLADVPGIDPHDLATIYMRRATIRWAFDRYPPAADDLRRAAELYRQAG
                     DELQATFAEANLGTVYLSMSRYGEAEALKLAAIRAAEEVNARHVLVSELGDLGVIYLG
                     MGRVELLLEYSNRMVKLATELGNDAELSRGRGNRASALLALGRAAEALPDIESSLSRY
                     HAQGRKEGAIVTTIDMVLYLEAIGDEAGAARLARENHAAALREDFPKLPIVTGRCLAP
                     FLPPAERRALLTEMLAAARAYGRPMDAAGCLFALAAIGADPAERAVHYREGVELVKQM
                     GATAWLAGRSPANPPWLPMMI"
     gene            complement(4579..6129)
                     /locus_tag="PK829_03990"
     CDS             complement(4579..6129)
                     /locus_tag="PK829_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HOU40412.1"
                     /translation="MNVDDITVSLLNATLRAWRRAEEPPATLLDLDFLRDTTAWAVAP
                     ASPAERDIQLRARVTSLAVAELDRLRAAESLPPTDPAATRVALLLALGRDFGGGNMAL
                     EEWSALYHRYLAPVSLSVAALAQAAHLDVRNFRHRVNNGARRLAEQLQQLEMAAQQRL
                     RRARLGRHLPPADYTRLFGVAEPQQQLVAALQPANGPDVISIEGLGGIGKTALAREAA
                     FVLAQGSDYDGIAWVSARQTWLNDAGAIETTPDAANTLADVIGRLATQLGFAELAGLS
                     VADKLDRLAPFLRAGRHLIVIDNLESVSDLELLLPALLPLARPTRFLLTSRQTLSHYP
                     LVTRFPVQLLSLADSRALVESELARHGHPARLAAEDMAALHEVVGGMPLALKLVAAQM
                     SLWPLPVLLDNLRRARHRAPEGLYRYIYWRAWQSLGDPARQLLLSLLPLAPDGEDVEW
                     LRLMSFLPPDEFGDALAQLRDASLLEVAGEPTSPRYRLHRLTATFLQTEILAAWGDSS
                     LQESLPNP"
     gene            6253..6480
                     /locus_tag="PK829_03995"
     CDS             6253..6480
                     /locus_tag="PK829_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU40413.1"
                     /translation="MNIPQERDGGLTAREREVLHLVAEGYTDAEIGRALHISIYTAQN
                     HVKSILGKLKARNRAHAGAMYVRANMADSSY"
     gene            6580..7416
                     /locus_tag="PK829_04000"
     CDS             6580..7416
                     /locus_tag="PK829_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40414.1"
                     /translation="MRQLYQMPSRTQLRALVLVLLLAVLTMGCALIERMRGDVILEGS
                     YDPIIIPITISINSLGEVTLEPRSEISVPTPIGVFHAGAHLEAHPFATSPQNVLVIRI
                     DDQECAYDLHGDRFEVDLNGGNFQLVSLKTEDDNIYIELRGDHHTGCTQDWSTVAASN
                     PSQGSGNEAGCPGASRSYMATGTRAYISVFQASVLREPSEFSSFARYKYLDSGNVVTV
                     IDGPVCGPGNPGHVLFWKVRSEVIDFADGTSDVVEGWVAEESGDIYLMRPVEQARSGP
                     ST"
     gene            7549..7806
                     /locus_tag="PK829_04005"
     CDS             7549..7806
                     /locus_tag="PK829_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40415.1"
                     /translation="MSAFEYLVVPFVGQLKSGVFSVENAQNVTAQLQAVISQYGQQGW
                     EFYRIDKINVQIKPGCLAALFGASASYITFDQIIFRHLTNP"
BASE COUNT         1388 a         2672 c         2592 g         1458 t
ORIGIN      
        1 ccggcgcgag ggcctggcca tccgcactgt gccgggggac atgcgcgacc tgagcgcctt
       61 cgccgacgcc accttcgacc tcatcttcca tcccgtgtcc aacatcttca cgcccgatgt
      121 gcgcgcggtc tggcgcgagt gctaccgcgt cctgcggccg ggtggcgcgc tgctgatggg
      181 cacggtcaac cccatcgagt acgccttcga ccgcgccctg gccgacgccg agggtatctt
      241 ccaactgaag cactctctgc cctactcgga cgtgaccgac atcagcacgg aggagcatgc
      301 ccgtctctac ggctcccacg caccctacga gttcagccac accctggaag accagatcgg
      361 cggccagcta gaggttggct ttgtcctgac tggcttcttt gaggcccaca aggaagacga
      421 ccggctcggc cgctacttcc ccaactacat cgccacgcgg gcgcttaagc cctacgccac
      481 gccggccgcg cccacggacg actgaaacga gcgggccaag acaatactga ttggagtatc
      541 tgtgacctac caacaactcg ttaccgacca ctctcgtgac ccggagcaac tcgaacaggc
      601 ctaccgcgcc gccgtcaagg ccggccaggg cgatgccttc cggcaggcca tcgacgccgg
      661 ccgcgcgtcc cagcccgaca acctgctcta cgcggcctgg cactaccggc tgcgcgatgt
      721 cgccgtcgct gtcaagactt cgttcgttcg ctggggttgg gccgtgccgc tggcgctgct
      781 caatggcctg ctgttctggt ggctgtccga cgaagagcgc tactctatcc agatcgccgg
      841 tggtccgccg ggcgcgtcca ccttcctgcc gaccctgctc ctgctggtcg ctccgctggc
      901 ggccgtcttc gtgctggcct acctggcccc ggtgcggcgg cgctggccgc tggccgtgct
      961 gatcggcgtc gggcttgtgg ccgccgccgg ctatgtgctc tgggtctacc cccgcctggg
     1021 cacgcgctcc ttccaggagc agtacgtcac cctgatggcc atgcacctgc ccctgctggc
     1081 ctgggcgggc gtggggctat acctcgtcgc cggccaccgc gaccccggca accgcttcgc
     1141 cttcctgatc aagtcgctgg aggtcgtcat catgggcggc ctgttcgcca tcgccggcgg
     1201 gctgttcacg ggcatcacca tcgccctctt ttccgctctg ggcatggagc cgtcggagtt
     1261 ggtcatgcgc ctgttcatcg ccggcggcgg cgggctgatc ccggttatcg ccgtggccgt
     1321 gatctacaac gccgcccggc cgccggcggg gcagtcgttc gaggacgggc tgagcaagct
     1381 gctggccacg ctgatgcgca cgctgctgcc gctgactctg gtggtgctgg tggtctacat
     1441 cggcttcgtg ctactcaact tccgcgagcc gttcgagaac cgtgacgtac tgatcatcta
     1501 caacggcatg ctcttcgccg tcatcgccct gctggtcggg gcgacgccgg tcagcctggc
     1561 cgagacctcg ccgcgagtgg ccaacgcgct gcgctgggct atcgtcgccg tggccgggct
     1621 ggcgctgctg gtcagcgtct acgccctggc ggccatcgtc taccgcacgg ccatggaccg
     1681 cctgacaccc aaccggctgg cgttcatcgg ctggaacgtc gtcaacatcg gcttgctggc
     1741 gctggtcgtc tgggccgggg cgcgcggccg ggcaacggac tggctgccgc ggctccaccg
     1801 cgctttcgcc atcggcaccg tcgcctatac cgtctggacg ctggccgtga tcctcgtcgt
     1861 gccgtggctg tttggcatcg accagggcgc gctcgacacg ctgccgccgg ccgtgcgcgc
     1921 ggtcgtctac gaatcccccg atcccatcct gctcaagtgc gccggcagcc cacacatcta
     1981 tctgctggac gacggcgaaa agcgctggat cgacaccatc gccactttca ccgagcgcgg
     2041 ctacgtctgg ggcgatgtcc acttcgttcc ctgcgatgag ctacacagcc tgcccgatgg
     2101 cgagccgatc ccggccgacg cggggccgcc gccgcagccg taagacatac ccggttctag
     2161 gcagcttcca tgctataatg gctgcctatg acgcggagcg attgacatga ccatgaatac
     2221 ggacctgaac ctcagcatga tgcgtgctca acttaccttg ttggaacgag agatgtccgc
     2281 gcttcggcgg ctgatcgacc aaatacaggc tccccagtcc caagtaacgt ttgagggcct
     2341 ggccggcgtg tggcagggca ctgtcttctc agacgaagat ctggcgtcct cgcgactgac
     2401 tatttcagag agcatctgat gcgttacgtc gtcgatacgc atgctctcgt atggtannnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctcg tatggtatct gagcaatgac
     2581 cctcggcttg gactggaggc ccggcgaatt cttgctgagc cttccacctc tctgattatt
     2641 cctacgcttg tcttggccga agccaagcac attagcgagc ggaagcgcac gacaatcaca
     2701 tttgatgaga tcatgcgaac ggtcgcatct gccaggcgaa tcacgatatt tcctctggac
     2761 attttcgtcg ttgccgccct gccaactaat ttggacattc acgacggctt gatcgttgct
     2821 acggctctag cagcgcgtga cctgttcgcc gaggaagttg ctgttcttac gcgcgatcag
     2881 ctcatttcag catccgcgct tgttccagtc atatggtgag ggtcacgtct tcttaaagga
     2941 agctcagtag gggcgcacct gcgtatgcgc ccggttgcgc gtccgcccgc catcatatca
     3001 tcatgggcaa ccacggcggg ttcgccggcg agcggcccgc cagccacgcc gtagccccca
     3061 tttgctttac gagttcgacc ccctcgcggt aatgcaccgc gcgctcggct gggtcggcgc
     3121 caatggcggc cagggcgaaa aggcagccgg ccgcgtccat cggccggcca taggcccgcg
     3181 ccgccgccag catctccgtc agcagtgcgc gccgctcggc cggcggcaag aacggggcca
     3241 ggcaccgtcc ggtcacgata ggcaactttg ggaagtcctc ccgcagcgca gcggcgtggt
     3301 tctcacgcgc cagccgggcc gcgcccgcct cgtcaccgat ggcctccagg tacagcacca
     3361 tgtcgatagt tgtcacgatc gcgccctctt tgcgcccctg agcgtggtag cggctcaggc
     3421 tcgactcgat gtccggcagc gcctcagccg cgcggcccaa tgccagcagc gccgaggcgc
     3481 ggttgccgcg cccgcggctc aactcggcgt cgttgcccaa ctccgtggcc agtttcacca
     3541 tccggttgga atactccagc aacagctcca cccggcccat tccgagatag atgacgccca
     3601 gatcgcccag ttcgctgacg agcacgtggc gcgcgttgac ctcctccgcg gcgcggatgg
     3661 cggctagctt caacgcctcg gcctcgccat agcgcgacat gctcaggtag accgtgccca
     3721 gattggcctc ggcgaaggtc gcttgcagtt cgtcgccagc ctggcggtag agttcggccg
     3781 cgcggcgcag atcgtcggcc gccggcggat agcggtcgaa cgcccagcga atcgtcgccc
     3841 gccgcatata gatcgtcgcc aggtcgtgag ggtcaatccc cggcacatcc gcgagccgat
     3901 cgatcaccgt ctcgctaagg gtcagggcca gttccggctg cgcggagtgg cgcagggtgt
     3961 ccatggtttc cagatcgagc cgggccgtgc ccattgctgc gcgggccggc ggttgggccg
     4021 cgggtagccg ggccaggtcg gcgcgcaact catccacgaa cgtctgcgcc tcgccatagc
     4081 gcgcctgcgc ccgcagcgtg gcggcgacaa cactgccggc caggcacagc ggccaggccg
     4141 ccccgctctc ctgtgccagg cgcagtgctt ccccggccgt gtcgcgcgcc ggttccagcc
     4201 ggccggtgtt cagcaacaag ccggcggcat gggccaaata ctcggcctgc tgggccggcc
     4261 ggcccagggc ggcggcgtgg tcggccgcct gctggagcgt agccagccag agcgaccact
     4321 ccccccagcg caccggcaac ggatgcagac gatccaccag cgccagccac aacggcctca
     4381 gttctgcccg ctggccggcg gcattgagca gggcaagaca gctgtcgaaa tgggggcgca
     4441 cctcagcggc cggccggccg tccacaaagt caaccgccag gcgtagctgg cccggcaaac
     4501 ggtcggcgta gaagcgccgc cagcgttggg cctcgtcgcc ccaggcgcgc ggcgtggcac
     4561 tttgccctgt tgcggcgttc atgggttcgg caggctctct tggagagaag agtcgcccca
     4621 cgccgccagg atttccgtct gcaggaaggt agccgtcagg cggtgcagcc ggtagcgggg
     4681 cgaggttggc tcgccggcca cttctagcag cgacgcgtcg cgcaattggg ccagcgcatc
     4741 gccgaattcg tcgggtggca gaaagctcat cagtcgcagc cactcgacat cctccccatc
     4801 gggagccagc ggcagcaggg agagcagcag ttgacgtgcc gggtcgccca gggattgcca
     4861 ggcgcgccag tagatgtagc gatacagccc ttcgggagcg cggtggcggg cgcggcgtag
     4921 attgtccaac agcaccggca gcggccacag gctcatttgc gcggccacca gcttcagcgc
     4981 cagcggcatg ccgccaacga cctcgtgcag cgcggccata tcttcggccg ccagccgggc
     5041 cgggtggccg tggcgcgcga gttcggattc caccagcgcc cggctgtcgg ccagcgagag
     5101 cagctgcacg gggaagcgcg tcaccagcgg atagtggctc agcgtctggc gactcgtcag
     5161 cagaaagcgc gtcggccggg ccagcggtaa cagcgccggc aacagcagtt ccaggtcgct
     5221 gacgctctcc agattgtcaa tgacgatcag gtgccggccg gccctgagaa agggggccag
     5281 ccggtcgagc ttgtcggcga cgcttagccc ggccagttcg gcgaagccca gttgtgtcgc
     5341 cagccggccg atgacatccg ccagcgtgtt ggcggcgtcg ggcgtcgtct caatggcccc
     5401 ggcgtcgttg agccacgtct gtcgcgcact gacccaggcg atgccgtcgt agtcgctgcc
     5461 ctgggccagg acgaaggcgg cttcgcgggc cagggccgtc ttgccgatgc cgcccagccc
     5521 ctcgatgctg atcacgtctg ggccgttcgc cggctgcaac gcggcgacca gttgctgctg
     5581 tggctcagcc acgccgaaga ggcgagtgta gtcggccggc ggcaggtggc gacccagccg
     5641 ggcgcggcgc agccgctgct gcgcggccat ctccagctgc tgtaactgct cggccagccg
     5701 ccgcgcgccg ttgttaacgc ggtggcggaa gttacgcacg tccaggtggg cggcctgggc
     5761 caacgctgca acgctcaacg acacgggcgc aagatagcgg tgatagagcg cgctccactc
     5821 ctccagagcc atgttgccgc cgccgaagtc gcggccgagg gccaggagca gcgcgacccg
     5881 cgttgccgct gggtccgtgg gcggcaacga ctcggccgca cgcaggcggt cgagttcggc
     5941 cacagccaga gacgtgacac gggcacgcaa ctggatgtcg cgctcagccg gcgaagcggg
     6001 cgcgacggcc caggcggtcg tgtcgcgcag gaagtccagg tcgagcaggg tcgccggcgg
     6061 ttcctcggcc cgccgccaag cgcgcagggt cgcattcaga agggagacag taatgtcatc
     6121 aacgttcacg tgacggctac ctcgatcaga agtcgtccga tgttgatccg atgtgtgccc
     6181 gattcctgta gaaaacggtg gagcactgtc tagtatagcc gtaagcgtcg ttggcgaaaa
     6241 atggagggtc gtatgaacat accacaagag cgcgacgggg ggttaactgc gcgggaacgc
     6301 gaggtgctgc accttgtggc cgagggctac acggacgccg aaatcggccg ggcgttgcac
     6361 atctccatct acaccgcaca gaaccatgtc aaaagcattc tgggcaaact gaaggcgcgc
     6421 aaccgggctc acgccggggc gatgtacgta cgggcgaata tggctgattc cagctattga
     6481 ggaagtagcc agccaccttt atactgttga tggggccgat ctcgattgcg cccgccaggg
     6541 tgttccagtt acggtgtcgc caccagaggg gaggcagacg tgcgtcagct ctatcagatg
     6601 ccctcgagaa cacaactcag agccttggtt cttgttttgc tattggctgt actgaccatg
     6661 ggctgcgcac tgatcgagcg gatgcgtggc gacgttatcc ttgagggttc ctatgaccca
     6721 attatcattc ccatcactat cagcatcaat agcctgggag aggtcacatt ggagccgagg
     6781 tccgaaatct cggtgcccac acccatcggc gtcttccacg ccggcgctca ccttgaggcg
     6841 catccgttcg ctacgtcgcc ccagaatgtc ctggtcatca ggatcgatga tcaggaatgc
     6901 gcctatgact tgcacggaga cagatttgag gtggatttga atggcggcaa tttccagctt
     6961 gtttccctaa agactgaaga tgacaatatc tacatcgagt tgaggggcga tcatcataca
     7021 ggttgcacac aggattggag cacagtcgct gcgtcaaatc catcccaggg ttctggcaat
     7081 gaggccggat gtcccggcgc ttcgcgctct tatatggcca ccggcacgag agcctacatc
     7141 tccgttttcc aggcttcagt cctccgggag ccaagcgaat tctcctcttt cgccaggtac
     7201 aagtaccttg acagtggcaa tgtcgtgacg gtcatcgacg gcccggtttg tgggccgggt
     7261 aatccaggtc atgtcttgtt ttggaaagtg cgcagtgaag tcatcgattt tgccgacggt
     7321 acaagcgatg tggtggaagg ttgggtggcc gaggagagcg gtgacatcta tttgatgcgg
     7381 ccggtggagc aggcacggtc agggccgtca acgtgacaga atgccagtgt acgcgtggcc
     7441 aaacacaatc agagcgcgag aggggcggcg attttgttcg gtgaacaaag tgcccgccgc
     7501 ccagctcgat ttcgtcgatg agcgaaggca gtatctcagg aggaaactat gagtgcattc
     7561 gagtatctgg tggtgccctt tgtgggccag ttaaagagcg gcgttttctc ggtcgagaac
     7621 gcgcagaatg tcaccgccca gttgcaggcc gtcatctcgc aatacggcca gcaagggtgg
     7681 gagttctacc gcatcgacaa gatcaacgtc cagatcaagc ccggttgcct ggccgcgctg
     7741 tttggggcca gcgcgtccta cattacgttc gatcagatca tctttcgcca tttaaccaat
     7801 ccgtaagtgc gcgaagatcg gctatcggca aggaagccca ggatgtgacc aagtacagtg
     7861 cctgtccaaa ctgcggtcgc aagccaacgt gggggcgcgt gcccatttac gaatgtagtc
     7921 ggtgtggcaa gcggttttgc tatgagtgcc ccggcagcaa cggcgcgcga cgctgcccca
     7981 actgtaagag cgagggtaag cggcacgtgg gggatgcaac ataggggaac gcagcttgct
     8041 tgccaagcac gccacgcgca acgaagtccg cagacccatg ccggcccgtc agggcctact
     8101 ccggtcagcc acgccgggaa ggactgccag acccgacaca tcgtctagac tggctccatc
     8161 cgcgccgaaa aatgccgtag cacctccggc gcgctgacga tccgcacgcc
//
LOCUS       DAOSMQ010000078         8076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8895_length_8076_cov_4.657524, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000078 DAOSMQ010000000
VERSION     DAOSMQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8895_length_8076_cov_4.657524"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PK829_04010"
     CDS             <1..934
                     /locus_tag="PK829_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOU40416.1"
                     /translation="ISKIAQLMPILDKVMRDPRPLVILAEKIEGAALSMLVHNHVNGL
                     FQCVAITAPGFGDRRLHKLEDIASVTGGAVFSKHSGFSLETMTIEQLGALKPEQISRL
                     TFHQLRALTATQLASLSDTQVGALNALQLASLGTERITALTTGQVDVLVSALVVAPER
                     TQDVAYTDSYFDAGQALFVPPGSAVAGMADLDGRTLAVELGALGHVEARAWQERLPGL
                     AVVTFGSADEALAAAASGAAGAALVDSVSGRLYLRDHAGSALLRLDPPVAPEPYAVAV
                     RIGDRTLHRRLNDALASLARSGQLEEITSRWLGP"
     gene            1053..2963
                     /locus_tag="PK829_04015"
     CDS             1053..2963
                     /locus_tag="PK829_04015"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOU40417.1"
                     /translation="MLPLLLFVVLLGLAPAALAQTKTFHWSQWDIDVVLQPDGRLAVT
                     ETQTLNFSGAPFTFGFRSIPVGRAGNNDGISDVSVREGNQAYTESFSNAPGTFEVVDE
                     GDETRINWYFDPALGEHTYTFSYIINGAVRMGTSEQGSGDQIFWTVIPSDVPARVDSS
                     RTTITLPEGVYPQRFTGTDNYLVEAYIADYTSSDVAITVSDDERVITYETLRPLMPGE
                     ALDVRVQFPHGLLAIPTPAWQASEQRDDVVGTVVLALSLLLLVAGPLGVVLLWYLRGR
                     DPQLGIVVPDYIAEPPDTLPPAMVGALLDEKVDMKDIMSTLVDLAHRGYVTMEEGERK
                     SYTFTRTAKPDSDLREFERTFLAKVFRGEESRTLSSLNNKFYSAIPQLRTQIEEAIIK
                     EGYLPRSPQSVRTSYTILAAVVLVLGIASLFFVGVFLGNNAGLSCFPMMAIVGTAIAL
                     FIAARHMPKKTPKGAEAAAKWNAFRTYLKNIRQYADVENSGDIFDKYLGYAVAFGLER
                     AFITTFAESPSTPIPTWYMPYPTVLHAPMGGGMGPFIGGRPQAGGAGSSGGGVPSLGD
                     ISGGLTGGLAGMSAGLTRMLNNTSTAMRSTPPPANAGSSGHFGGGGGFSGGFSGGFGG
                     GSSGGGGSAGFG"
     gene            3009..3524
                     /locus_tag="PK829_04020"
     CDS             3009..3524
                     /locus_tag="PK829_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40418.1"
                     /translation="MGRTLGIILIIGGIVVGAIVVALMIVYRNEGSLSSGAATLGIAL
                     GLLALSLPQLGFGAYLLMRGQQEAAVAETAGKQRQLLGMVKARGQVQIADLAIELRTS
                     RDELQKMIYELVNMGLYSGYVNWAEGTLYSSQASELRQLDRCKNCSGQLELAGKGVIR
                     CPYCGTEYFLP"
     gene            3627..4199
                     /locus_tag="PK829_04025"
     CDS             3627..4199
                     /locus_tag="PK829_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40419.1"
                     /translation="MTDETQDLYDMIAGERGMLSKLLSKIPGFKGYMEKESRRDADQL
                     LRDTISGRLQQTRLDLADVLRTLSRDIILGIEYAEPIGRADNQLMGLINKIKDAPQGY
                     AGFFDAVKVKEEDLARLYAFDEQMLTQAEQIAADVAALHKTVENSLNIHDAIVTLTNT
                     LRAANEAFNSRQEVLNQVGVDTIAKQNTQE"
     gene            4221..5354
                     /locus_tag="PK829_04030"
     CDS             4221..5354
                     /locus_tag="PK829_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU40420.1"
                     /translation="MPKILDRVAVEGWGRDEVVQKFPRGGLGDIKLGSQLIVNPGETA
                     VFVRGGEALGTFEPGRYTLTTENIPFLTDLFEKGLFGGANVFTADVYFVKTTDMTLKW
                     GTVNPIIVEHPQRMPGASAIVGNGTYVVKVKEPWRFLNAMDAFRESVQQTDIKHRLDP
                     MLAVMIADKLSELAAAKNLGPAQLQSFTKDLNDLLVALLQEEFDAIGMTLVTFAINMT
                     LHPDSLKVVTNMGYGTSYVQKQQADALMAAASNPSGGALSEVGFGAMGMAAMQQQQMQ
                     QQLMQQQWLAQQQAGQGGTTPPAGTGAPTSGAPTGGAPTGGAPTGGAMPMPDVMTPEQ
                     AADFLQVTPDDIIAAIDAGELKAKKIGAGYRISKANLEAYLAG"
     gene            5541..5987
                     /locus_tag="PK829_04035"
     CDS             5541..5987
                     /locus_tag="PK829_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU40421.1"
                     /translation="MYKTILVPLDGSSLAETILPHVEELAGLCGASVIFLNVVELPHI
                     MDKGVTYEGLPQKTATDVEAEVDEARRYLDELTAREAARGLQVRGEVEHGPVVATILR
                     VATANAADLIAMASHGRGGLTEVYYGSVAAGILQRIDRPLLIVRGQ"
     gene            6041..7906
                     /locus_tag="PK829_04040"
     CDS             6041..7906
                     /locus_tag="PK829_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU40422.1"
                     /translation="MNQPAWLRSAHHDGSERYVSKLHPRLGETVRLRLRVAQAAPVRR
                     VLLRTFPDGEQALTAMALSQAEPFVQWWQADLLIDEPLAHYRFVIEAADGLWHYSAAG
                     PSAHVPLDYTDFRILADYDPPGWPAESVFYQIFPDRFANGDPSTDPRPEEYEYRGHRP
                     TTYPWGATPPAGQLFPLVFYGGDLPGIESRLDYLAGLGVNALYLNPIFTALSNHKYDV
                     ADYDHVDPHFGGDAALVSLRRALDARGMRYLLDIVPNHCGYWHPWFQAARADSAAPEA
                     EFFTFRHHPDDYATWLGVWTLPKLNYLSAELRRRIYAAPDAVFRRWLRPPFSADGWRI
                     DVANMLGRQGPTQLGREISAGIRRAVRQTRPDAYLIGENFFDATSQLQGDQWDGVMNY
                     DGFTQPLWYWLRGYDLGAHGFADKLTGPHWPTEALAASWQSRLATIPWAVALQQYNLL
                     DSHDTPRIRTTVGENDALHRLAAVVLLTFPGVPGLYYGDEIGMTDVERLGARGCMVWD
                     ETRWNQPLHAFHRDLIHLRRRSSALQRGGFQLLLVEDDTIAYQRDSADERILVFAHRG
                     AAPRPAGGVPIDHAGVPDGARFVDALGGAEFVVANGTLPLPELPQGALICQQIAP"
BASE COUNT         1401 a         2734 c         2547 g         1394 t
ORIGIN      
        1 gatctcgaag atcgcgcagc tcatgccgat cctcgacaag gtcatgcgcg acccgcgccc
       61 gctcgtcatc ctcgcggaga agatcgaggg cgccgcgctg agcatgctcg tgcacaacca
      121 cgtcaacggg ctcttccagt gcgtcgcgat caccgccccc gggttcggcg accgccggct
      181 gcacaagctc gaggacatcg cgtccgtcac cggcggcgcc gtcttcagca agcactccgg
      241 cttctcgctc gagacgatga cgatcgagca gctcggcgcg ttgaagcccg agcagatctc
      301 gcggctgacc ttccaccagc ttcgggcgct gaccgccacg cagctcgcca gcctctcgga
      361 cacccaggtc ggggcgttga acgctctcca gctggcctcg ctcggcacgg agaggatcac
      421 cgcgctgacg accgggcagg tcgatgtcct tgtgtcggcg ttggtcgtcg ccccggagcg
      481 cacgcaggat gtggcctaca cggacagcta cttcgacgcc gggcaagcgc ttttcgttcc
      541 gcccggctcg gccgtcgccg gcatggccga cctcgacggg cgaacgctgg ccgtggaact
      601 gggcgcgttg gggcacgtgg aagcgcgggc gtggcaggaa cggctgccgg ggctggcggt
      661 ggtcacgttt ggcagcgctg atgaggccct tgctgccgcg gcctccgggg ccgccggcgc
      721 ggcgctggtg gacagtgtca gcggccggct ctacctgcgc gaccatgccg gcagcgccct
      781 gcttcgcctc gatccgcccg tagccccgga accttacgcc gtggccgtcc gtatagggga
      841 tcggacgctc catcgccgcc tcaacgacgc tctggcctcg ctggcgcgtt ccggtcaact
      901 agaggaaatc acaagccgct ggttagggcc gtgacaataa tccgcttttc gtggaagttg
      961 gtctataata cagatgtggg ccattccgaa gtgtaagcta ggaaggacat tgcgtgatgt
     1021 tttcccggac ttattccgtc agtaaacgtg ttttgctgcc ccttctcctg ttcgtggtgt
     1081 tgctaggtct ggcgccggca gcgctggcgc agacgaagac ctttcactgg agtcagtggg
     1141 acatcgacgt cgtcttacag cccgacggcc gcctggccgt cacggagacg cagacgctga
     1201 acttctccgg cgcgccgttc actttcggct ttcgcagcat tcctgtcggc cgggcgggca
     1261 acaacgacgg catcagcgac gtcagcgtcc gcgagggcaa tcaggcctat accgaatcat
     1321 tctccaacgc gccgggaacc ttcgaggtcg tcgacgaagg cgatgagacg cgcattaact
     1381 ggtatttcga ccccgcgttg ggcgagcata cctatacctt cagctacatc atcaacggcg
     1441 cggtgcgcat gggtacctct gaacaagggt cgggcgacca gatcttctgg acggtcatcc
     1501 ccagcgacgt gcctgcccgc gttgacagca gtcggacgac catcactctg cccgagggcg
     1561 tctacccaca gcgcttcacc ggcacggata attacctcgt cgaagcctat attgctgatt
     1621 acacctcgtc cgacgtggcc atcaccgtca gcgatgacga gcgggtgatc acctatgaga
     1681 cgttgcggcc gttgatgcct ggcgaagcgc tggacgtgcg cgtccaattc ccccacggcc
     1741 tgttggccat ccccacgccc gcctggcagg cgtcggagca gcgtgacgac gtcgtcggca
     1801 cggtggtgct ggcgctgtcg ctgctgctgc tggtggccgg gccgctgggc gtggtactgt
     1861 tgtggtatct gcgcggccgc gacccgcaac tgggcattgt cgtgcccgat tacatcgccg
     1921 agccgcccga cacgttgccg ccggcgatgg tcggcgcgct gctcgacgag aaagtggaca
     1981 tgaaggacat catgtctacg ctcgtcgatc tggcccatcg cggctatgtg acgatggagg
     2041 agggcgagcg caagagctac accttcaccc gcacggccaa gccggacagc gatctgcgcg
     2101 aattcgagcg taccttcctg gccaaggtct ttcgcggcga ggaatcgcgc acgcttagca
     2161 gcctgaacaa caagttctac agcgccatcc cccagttgcg cacccagatc gaagaggcca
     2221 ttatcaagga aggctatctg ccgcgctcgc cgcagagcgt gcgcaccagc tacaccattt
     2281 tggcggccgt tgtgctggtt ctgggcatcg ccagcctgtt cttcgtcggc gtgttcctgg
     2341 gcaataacgc cgggctgtcc tgcttcccga tgatggctat tgtcggcacg gccatcgccc
     2401 tgttcattgc cgcccgtcac atgcccaaga agacgcccaa aggagccgag gcggcagcca
     2461 aatggaacgc cttccgcacc tatttgaaga acatccgcca gtatgctgac gtggaaaact
     2521 ccggcgacat cttcgacaaa tacctcggct acgccgtcgc ctttgggctg gagcgcgcgt
     2581 tcatcacgac ctttgccgag tcgcccagca cacccatccc gacgtggtac atgccctatc
     2641 cgaccgttct gcacgcgcct atgggcggcg gcatggggcc gttcatcggc ggccgcccgc
     2701 aggctggcgg cgcgggcagc agcggcggcg gcgtgccttc gctgggcgac atttccggcg
     2761 gcctgacggg tggcctggcg ggcatgtcgg ccgggctgac gcgcatgctt aataacacgt
     2821 ccacggccat gcgcagcacg ccgccgccgg ccaacgccgg cagtagcggc cattttggcg
     2881 gcggtggcgg cttcagcggt ggtttcagcg gcggcttcgg cggcggctcg tccggcggcg
     2941 gcggcagcgc tggcttcggc taagccgacg ccgtcgcgct ggacatgacc ttagcattgg
     3001 aggtgtgaat gggaaggacg ttggggatca ttctgatcat cggcggcatc gtcgtcggag
     3061 cgattgtggt ggccctcatg atcgtttatc gcaacgaggg cagcctgagt tctggcgcgg
     3121 ccacgctggg catcgctctg gggctgctgg cgctgtcgct gccacaactg gggttcggcg
     3181 cgtatttgct gatgcgcggg caacaggaag cggccgtggc cgagacggcc ggcaagcaac
     3241 gccaactgct gggcatggtc aaggctcgcg gtcaggtgca gatcgccgac ctggctatcg
     3301 agctgcgcac gtcacgcgac gagctgcaaa agatgatcta cgaactggtc aatatgggcc
     3361 tctacagcgg ctatgtgaac tgggccgagg gcacgctcta ttccagccag gccagcgagt
     3421 tgcgtcaact cgatcgctgc aagaactgta gcgggcaact ggaactggcc ggcaagggcg
     3481 tcatccgctg cccctattgc ggcacggagt acttcctgcc ataacgatgg ttcaagcaga
     3541 tgttggcaat ggttagcggc gtgggcctgt tggtctgggg agtgggctaa gccgcgctgc
     3601 gcgagatgga tgaaggagaa cgaccaatga cggatgagac tcaagatttg tatgacatga
     3661 tcgccggcga gcgggggatg ctcagcaagc tcctgagcaa aatccctggc ttcaaggggt
     3721 atatggaaaa agagagccgc cgcgacgcgg atcagttgct gcgcgacacg atcagcgggc
     3781 gtttgcaaca gacacggctc gatctggccg acgttctgcg cacgctcagc cgcgacatca
     3841 ttctgggtat cgaatatgcc gagccgatcg gccgggccga taaccagttg atgggtctga
     3901 tcaacaagat caaggacgcc ccacagggct acgccggctt cttcgacgcg gtcaaggtca
     3961 aggaagaaga cctggcccgt ctatatgcct tcgacgagca gatgctcacc caggccgagc
     4021 agatcgccgc cgacgtggcc gcgctgcaca agacggtcga gaacagtctg aatatccacg
     4081 acgccatcgt caccctgacc aacaccctgc gcgcggccaa cgaagctttc aactcgcgcc
     4141 aggaggttct caaccaggtt ggcgtcgata cgattgccaa gcagaacacg caggagtaaa
     4201 tcacaggagg tcgatgagcg atgcccaaga tactagatcg tgtagctgtt gaagggtggg
     4261 gacgcgatga agtcgtccag aagtttccgc gcggtgggct gggcgacatc aagctcggct
     4321 cgcagctgat cgttaatccc ggcgagacag ccgtctttgt ccgcggcggc gaggccctgg
     4381 gcacgtttga gccggggcgc tacaccctga ctaccgagaa tatccccttt ctgacggact
     4441 tgtttgaaaa gggcctgttc ggcggggcca acgtgtttac ggccgacgtc tacttcgtca
     4501 aaacgacgga catgacgctt aaatggggca cggttaaccc catcatcgtc gagcatccgc
     4561 agcgcatgcc cggcgccagc gccatcgtcg gcaacggtac ctatgtcgtt aaggtcaagg
     4621 aaccgtggcg cttcctcaac gccatggatg ccttccgcga gagcgtccag cagaccgaca
     4681 tcaagcaccg ccttgacccg atgctggcgg tgatgatcgc cgacaagttg agcgaactgg
     4741 ccgcggccaa gaacctcggc ccggcgcaac tccagtcctt caccaaagac ctcaacgacc
     4801 tgctcgtcgc cctgctgcaa gaggagttcg atgccatcgg catgacgctg gtgaccttcg
     4861 ccatcaacat gacgctgcac ccggactcgc tcaaagtcgt caccaacatg ggctatggca
     4921 cgagctatgt ccagaagcag caggccgacg ccctcatggc cgcggccagc aacccgtccg
     4981 gcggcgcgct aagcgaggtc ggcttcgggg cgatgggtat ggccgccatg caacagcagc
     5041 agatgcagca acagctcatg caacagcagt ggctggcgca gcagcaggcc ggacagggcg
     5101 gcacgacacc cccggccggc acaggcgcgc ccacgagtgg cgcgcccacg ggcggcgcgc
     5161 ccacgggcgg cgctccgacc ggcggcgcga tgcccatgcc tgatgtcatg accccagagc
     5221 aggcggccga tttcctacaa gtgacgcccg atgacatcat cgccgccatc gacgctggcg
     5281 agctgaaggc caagaagatc ggcgcaggct atcgcatcag caaagccaac ctggaggcct
     5341 acctggccgg ctaggtctgc ccgcccggcg cgccggaaca gcttacgcat caggccggct
     5401 gacgtccgtc cctgcacgcg cccagacctc agaggtcttc attggcctct gaggtctgat
     5461 ctattcttgg ccaaacggca gcgacgacgt aaagtagagg ccatcgctgc ctctccgcgg
     5521 cagcccactc aggagtcacg atgtacaaaa cgatcctggt ccccctggat ggctcgtccc
     5581 tggccgagac catcctgccc cacgttgaag agttggccgg cctgtgcggt gcgtcggtca
     5641 tcttcctgaa cgtcgtcgaa ctgccccaca tcatggacaa gggcgtgacc tatgaggggc
     5701 tgccccagaa aacggccacc gacgtcgaag cggaggtcga tgaggcgcgc cgctatctgg
     5761 acgaactgac tgcccgggag gcggccagag ggctgcaagt gcgcggcgaa gtggagcatg
     5821 ggccggttgt ggccacgatc ctgcgcgtgg ccaccgcgaa cgccgccgac ctgatcgcca
     5881 tggccagtca cggccgcggc ggcctgaccg aggtctacta tggcagtgtc gccgccggca
     5941 tcttgcagcg catcgaccgg ccgctgctca tcgtccgcgg gcagtagcag ccggccgcca
     6001 ggcgagccgg ccacacttct gacacccctg ggcttaccct atgaaccaac ccgcctggtt
     6061 gcgctccgcc catcacgacg gctccgagcg ctacgtctcc aagctccatc cacgtctggg
     6121 cgaaacggtg cggctgcgcc tgcgcgtcgc ccaggcggcc cccgtgcgcc gcgtcttgct
     6181 gcgcaccttc cccgacggcg aacaggcgtt gacggcaatg gcactgagcc aggccgagcc
     6241 gttcgtccaa tggtggcagg ccgacctgct catcgacgaa ccactggccc actaccgttt
     6301 tgtcatcgag gcggccgatg gcctgtggca ctacagcgcc gccggcccat cggcccacgt
     6361 gcctctcgac tacaccgact ttcgcatcct ggccgactat gatccgcccg gctggccggc
     6421 cgaatccgtc ttctaccaga tcttccccga ccgtttcgcc aacggcgatc cctccaccga
     6481 cccgcgaccg gaggagtacg agtaccgcgg ccaccgcccc accacctatc cctggggcgc
     6541 gacgccgccg gccgggcagc tcttcccgct ggttttctac ggcggcgacc tgcccggcat
     6601 cgaatcgcgc ctggactatc tggccggcct gggcgtcaac gctctctatc tgaacccgat
     6661 cttcacggcg ctatcgaacc acaagtacga cgtggccgac tatgaccacg tggatcccca
     6721 cttcggcggc gacgcggctc tcgtctcgct gcggcgggcg ctggacgcgc gcggcatgcg
     6781 ctacctcctc gacatcgtac ccaaccactg cggctactgg catccctggt tccaggcggc
     6841 gcgggccgac agcgccgcgc cggaagccga gttcttcact ttccgtcacc atcccgatga
     6901 ctatgcgacg tggctcggcg tctggacgct ccccaagctc aactacctca gcgccgaact
     6961 gcgccgccgt atctacgccg cccccgacgc cgtcttccgg cgctggctgc ggccgccgtt
     7021 ctccgccgac ggctggcgca ttgatgtcgc caatatgctc ggccggcagg ggccgaccca
     7081 actcggccgc gagatcagcg ccggtatccg ccgcgccgtg cgccagaccc gccccgacgc
     7141 ctatctcatc ggcgagaact tcttcgacgc caccagccag ctccagggcg accagtggga
     7201 tggcgtgatg aactacgacg gcttcaccca accgctgtgg tactggttgc gcggctatga
     7261 tctcggcgcg catggctttg ccgacaagct gactggcccc cactggccga ccgaggcgct
     7321 ggccgccagc tggcagagcc gcctggcaac catcccctgg gccgtggccc tacagcaata
     7381 caacctcctc gacagccacg acacgccgcg catccgtaca actgtgggcg agaacgacgc
     7441 tctccaccgg ctggccgcgg tcgtgctact gaccttcccc ggcgtgcccg gcctctacta
     7501 tggcgacgag attggcatga ccgacgtgga gcgcctcggc gctcgcggct gcatggtctg
     7561 ggacgagacg cgctggaacc agccgctgca cgcctttcac cgcgacctca tccatctgcg
     7621 ccgccgctcg tcggccctcc agcgcggcgg cttccagctg ctactggtgg aggacgacac
     7681 catcgcctac cagcgcgata gcgctgacga gcgtatcctc gtctttgccc accgcggcgc
     7741 cgcgccgcgc ccggccggcg gcgtgcccat cgaccatgcc ggcgttcccg acggggcgcg
     7801 ctttgtggat gcgctcggcg gggcggagtt cgtcgtcgcc aatggcacgc tgccattgcc
     7861 ggagttgccg cagggggcgc tcatctgcca gcagatcgcg ccgtagcatg gccgcccgcc
     7921 tcgcccccgc cctactggcc cgcgcctacg atctgctcta cggccgtctg gcctggactt
     7981 atgacgccgt cgcctgggcc gtctcgctgg ggcagtggca ggcctggggc gcggcggccc
     8041 tgccctatct gaccggcccg cgcgtgttgg aactgg
//
LOCUS       DAOSMQ010000079         8069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8905_length_8069_cov_5.576741, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000079 DAOSMQ010000000
VERSION     DAOSMQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8905_length_8069_cov_5.576741"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="PK829_04045"
     CDS             complement(<1..1405)
                     /locus_tag="PK829_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOU40423.1"
                     /translation="MLHERNAAEGHIATELQVRPEQVAAAVSLLDDGNTIPFIARYRK
                     EVTGVLDEEQLRYIAEEIARRRALDERRAAVLASVEEQGKLTEELRGQIEAAMTMTLL
                     EDLYAPYRPKRRTRAGIAREKGLGPLADLILAQPRTSQTAAQLAESYLSEAAPTTEAA
                     LAGARDIVAETISDHAGVRGRLRQRAMQVGVLCARRIEGAADERGIYSLYYDFEMAVP
                     RLRPHQVLAINRGEADKVLRATVNVTEAEQLLAMRAHFRPDRLSPLAEHLQLAMDDAA
                     KRLLLPAVERDARRALSEMAERHDIEVFASNLRALLTQPPLAGHVVLGVDPGFRTGCK
                     LAVIDPTGKVLDTGTIYPHTSPHRAAEAEQALLTLIARHGVTLIAIGNGTASRETERL
                     VAGLTRERAGLHYVMVSEAGASVYSASPLARAEMPDLDVSLRGAVSIGRRLQDPLAEL
                     VKIDPKSIGVGQYQHDVN"
     gene            1503..2240
                     /locus_tag="PK829_04050"
     CDS             1503..2240
                     /locus_tag="PK829_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU40424.1"
                     /translation="MSYQVLVVEDDETLRDTLVYNLQNQGYEITTADNGYAALELARR
                     QQPDLLLLDLMLPGLNGFEVCRILRKEMSLPILILTARAEEIDKVIGLEVGADDYLTK
                     PFSMRELLARVKALLRRVEMIRGELIAQDEAAEAARPGMAALPVEPLTFGNLTIDETR
                     RETRLDGHPIRLKPKEFDLLLFLARHRGIALSRDLILERVWGWSFDGNSRTVDVHIRW
                     LREKIEPDPANATRIITVRSIGYRFDG"
     gene            2244..3635
                     /locus_tag="PK829_04055"
     CDS             2244..3635
                     /locus_tag="PK829_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU40425.1"
                     /translation="MATRLFRRIFVSFALLTIVLTLGLYLYLTRSSCLANPSCPPRAL
                     LLASGLGLLGVAGLSFWLAQRTVRPAQQVADAARRIAAGDDAARVLPDSRHTASGLVS
                     AFNDMAERQRERINDLVRDHRRFATVLEHMADGVLITDHLSMVLLINPAAGRLLRTTP
                     EEALGRPFAAVVRHHSLIELWQRCRAQEQEQVEAVELGPELLLQVVVTPFRESGTAGY
                     VVIFQDLTQVRRLQTMRRDFLGNLSHELRTPLASLRAVIETLQDGALADPPAAERFLR
                     QAENEVHTITQMVAEMTELSQIESGQVSLRLVPVAVARLFEVPLERLGLQAERAGAQL
                     SVEVAAGTPPVLADEERIQQVLTNLLHNAIKFTPSGGYIRLSARPQPDNAGEVLFEVR
                     DTGIGIAKADLPRVFERFYKSDRARTRSRGGTGLGLAIARHLVEAHGGRIWVKSKIDK
                     GSSFYFTLPTAGQ"
     gene            3863..5863
                     /locus_tag="PK829_04060"
     CDS             3863..5863
                     /locus_tag="PK829_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU40426.1"
                     /translation="MRSKLLALLILVTLMAAACGGTAQQAEPETTAPTATTVDETAAT
                     AEPADAAEPTAASATSAEGEGTMPLADPLAVSGDIVTAGSSTVYPLAEAIAERYQAEG
                     FSGQITIDSIGTGGGFERFCGTGETDIANASRAIKDEEAANCAAIGRTPVEFRIGTDG
                     LAVVVSQENDFVQDLTLEQLALVFSTAETWADVDPSFPDEPIERYIPGTDSGTFDYFT
                     EVIYPDLEDGVANPQLEAANLQQSEDDNVLVQGVAGSPYAIGYFGYAYAANNADTVRL
                     VAVEGVVPDFDTVEGGTYALARPLFLYSDAAILQAKPQVADYINFFLTNVDEEIETVG
                     YFPASDEALNEARNHWLVAMGLAATMPENTTGETVAQGAMCELPAADPLAVSGDIVTA
                     GSSTVYPLAEAIAERYQAEGFSGQITIDSIGTGGGFERFCGTGETDIANASRAIKDEE
                     AANCAAIGRTPVEFRIGTDGLAVVVSQENDFVQDLTLEQLALVFSTAETWADVDPSFP
                     DEPIERYIPGTDSGTFDYFTEVIYPDLEDGVANPQLEAANLQQSEDDNVLVQGVAGSP
                     YAIGYFGYAYAANNADTVRLVAVEGVVPDFDTVEGGTYALARPLFLYSDAAILQAKPQ
                     VADYINFFLTNVDEEIETVGYFPASDEALNGASCAFVDATGQ"
     gene            6049..7026
                     /gene="pstC"
                     /locus_tag="PK829_04065"
     CDS             6049..7026
                     /gene="pstC"
                     /locus_tag="PK829_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOU40427.1"
                     /translation="MENLTVLPPRGEAGAGRAGAASASDPPDLSRRHRIGERTIESGL
                     LLAAAVSILTTIGIVAILLEEALRFFADERVTVVNFLTDTTWQPQASHFGILPLLNAT
                     LMITAIALVVAIPLGLASAIFLSEYASPRVRSLLKPALEMLAGVPTVVYGYFALTFVT
                     AILRAVLGKDIVQIYNVASAGLVVGVLITPLIASLSEDALHAVPNSLREAAYGVGATK
                     FETAVKVILPAALSGVAAALVVAISRAFGETMVVAIAAGAGPKLTWNPFESAETMTGY
                     MVRISGGDLSYESLDYKSIYAIGLVVFAVTLLLNIISQRIVARFREVYE"
     gene            7023..>8069
                     /locus_tag="PK829_04070"
     CDS             7023..>8069
                     /locus_tag="PK829_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU40428.1"
                     /translation="MNDHQSPAESAPEPGPQPSDRFVDLYPSEDGIRAQVTRRQRVAT
                     SWRFVFLAATSLAVVILSTLLLSIINQSFGLVAEQTDIPEAQLVAGYYKSQLTGATNV
                     TLSTEDDAAIAAGIAADANGIGLLGYALYTANQPALQALSIDGIAPSAETVTAGTYPL
                     TRPLLLYSSAAILSDKPQVAAFLLYYLQHAEEVMSEQGAFPADAAALAEQAAALATML
                     GLDATTPIDPAAYEGDIVISGSSTLNPVTREVARRFRSEGFLGALKISSVGTVAGFEA
                     FCAESADLVDASRPITTVESAACRANGLTPLSVQVGLDPMVVAVNSANTAVTNVTGQI
                     MQRPLGVAELLPQRH"
BASE COUNT         1359 a         2738 c         2576 g         1396 t
ORIGIN      
        1 ggttcacgtc gtgctggtac tggcccacgc cgatgctctt ggggtcgatc ttcaccagct
       61 cggccagcgg gtcttgcagg cggcggccga tcgacaccgc gccgcgcaac gacacatcca
      121 ggtcgggcat ctccgcgcgc gccagtgggc tggcgctgta gacgctggcc ccggcctcgc
      181 tgaccataac gtaatgcagg ccggctcgct cgcgcgtcag gccggccacc agccgctccg
      241 tctcgcgcga ggccgtgccg ttgccgatgg cgatgagggt gacaccgtga cgggcgatga
      301 gggtcagcaa cgcctgctcg gcctcggcgg cgcggtgcgg cgacgtgtgg ggatagatcg
      361 tccccgtgtc cagcaccttg cccgtcgggt cgatgacggc cagcttgcag ccggtgcgaa
      421 agccgggatc aacacccagg acaacgtggc cggccagcgg cggctgggtc agcagggcgc
      481 gcaggttgct ggcgaagacc tcaatgtcgt ggcgctcagc catttcgctc agggcgcggc
      541 gcgcgtcccg ctcgacagcg ggcagcagca gccgcttggc ggcgtcgtcc atggccagtt
      601 gcagatgctc ggccagcggc gacagacggt cgggccggaa gtgcgctcgc atggccagca
      661 gttgctccgc ttccgtcacg ttgacggtcg cgcgcagcac cttgtccgcc tcgccccggt
      721 tgatggccaa gacctggtgc ggacgcaggc gcggcacggc catctcgaag tcgtagtaga
      781 gcgaatagat gccccgctcg tcggccgcgc cctcaatgcg ccgcgcgcag aggacgccga
      841 cctgcatggc ccgctgtcgc aggcggccac gcacgccggc gtggtcgctg atggtctcag
      901 cgacgatgtc gcgcgccccg gccagcgctg cctcggtcgt cggcgcggcc tcgctcaggt
      961 acgactcggc cagttgcgcc gccgtctggc tcgtgcgtgg ctgggccagg atgagatcgg
     1021 ccagcggccc caaccccttc tcgcgggcga tgccggcgcg ggtgcggcgc ttgggccggt
     1081 agggggcgta gaggtcttcc agcagcgtca tcgtcatcgc cgcctcgatc tgcccgcgca
     1141 actcctccgt cagcttgccc tgctcctcaa cggaggccag gacagcggcg cggcgctcgt
     1201 ccagggcgcg ccgacgggcg atctcctcgg cgatgtagcg cagttgctct tcgtccagga
     1261 cgcccgtgac ctctttgcga tagcgggcga tgaaggggat ggtgttgccg tcgtccagca
     1321 gactgacggc cgcggcgacc tgttccggtc gaacctgtaa ctcagtggcg atgtggccct
     1381 cggcagcgtt gcgttcatga agcatggtgg tattctccga ctggcaccga tcatacgcgg
     1441 cattatccgc ctgtccagac ctgggacaaa cgactctggc tgcgcggcta ccgaaagcgc
     1501 ttatgtccta ccaagtcctt gtcgtcgaag atgatgagac cctgcgcgat acgctggtct
     1561 acaacctcca gaaccagggc tacgaaatca ccaccgccga caacggctac gcggcgctgg
     1621 agctggcccg ccgccagcaa cccgacctgc tgctgctcga cctcatgctg ccgggcctca
     1681 acggcttcga ggtctgccgc atcctgcgca aggagatgag cctgcccatc ctcattctga
     1741 cagcgcgcgc ggaagaaatc gataaggtca ttggcctgga ggttggcgcg gacgactatc
     1801 tgaccaagcc gtttagcatg cgcgagctgc tggcccgcgt caaggcgcta ttgcgtcgtg
     1861 tggagatgat ccgcggcgag ctgatcgccc aggacgaggc cgctgaggcc gcccgcccgg
     1921 gcatggccgc cttgcccgtc gagccgctga ccttcggcaa cctgactatc gacgagaccc
     1981 ggcgcgagac ccgccttgac ggccacccca tccgcctcaa gccaaaagag ttcgatctgt
     2041 tgctcttcct ggcccgccat cgcggcatcg ctctgtcgcg cgacctgatc ctggagcgcg
     2101 tctggggctg gtcgttcgac ggcaacagcc gcacggtcga tgtccacatc cgttggctgc
     2161 gcgagaagat tgagccagac ccggccaacg ctacgcgcat catcaccgtg cgcagcattg
     2221 gctatcgctt tgatgggtaa gacatggcca ccaggctgtt tcggcgcatc ttcgtttcct
     2281 tcgcgcttct gaccattgtc ctgaccctgg gtctatacct gtacctgacg cgcagcagct
     2341 gtttggccaa tcccagttgc ccgccgcggg cactgctgct ggcctctggg ctagggctgt
     2401 tgggtgtagc cgggttgtcg ttttggctgg cccagcgtac ggtccggccg gcccagcaag
     2461 tcgctgatgc tgcgcgccgc atcgccgccg gcgacgacgc cgcgcgcgta ctgcccgata
     2521 gccgccacac cgccagtggc cttgtcagtg ccttcaacga tatggctgag cgccaacgcg
     2581 agcgcattaa cgacctggtg cgcgatcacc gccgctttgc taccgtgctg gagcacatgg
     2641 ccgacggcgt tctcattacc gaccatctta gcatggtctt gctgatcaac ccggccgccg
     2701 gccgcctgct gcggacaacg ccggaagagg ccctcggccg tccctttgct gccgtcgttc
     2761 gccaccacag tctgatcgaa ctgtggcagc gctgtcgcgc ccaggagcag gaacaggtcg
     2821 aggcggtcga actggggccg gaattgctgc tgcaagttgt cgtcacaccc tttcgcgaga
     2881 gcggcacggc cggttacgtg gtcatcttcc aagacctcac ccaggtacgg cgcttgcaga
     2941 cgatgcgccg cgatttcctg ggcaacctgt cgcacgaact gcgcacgccg ctggcctccc
     3001 tgcgcgctgt catcgagacg cttcaagacg gcgcgctggc cgacccgcca gcggccgagc
     3061 gctttctgcg ccaggccgag aacgaagtcc acaccataac ccaaatggtg gccgagatga
     3121 ccgagctgtc gcagatcgag tcgggtcagg tgagcctgag gcttgtccct gtggccgtgg
     3181 cccgcctctt cgaagtgccg ctggagcggc tggggctaca ggctgaacgg gcgggagcgc
     3241 aactgagcgt cgaagtggct gccggcacgc cgccggtgct ggccgacgag gagcgcatcc
     3301 agcaggtact caccaatctt ctccataacg ccatcaagtt caccccgtcg ggcggctaca
     3361 ttcgtctgag cgcccggccg cagcccgaca acgccggcga agtgctcttc gaggtgcgcg
     3421 acaccggtat cggcattgcc aaggccgact tgccgcgcgt cttcgagcgc ttctacaagt
     3481 ctgaccgggc gcgcacccgc agccgtggcg gcactggcct ggggcttgcc atcgcccgcc
     3541 atctcgtcga agctcacggc ggccgcatct gggtcaagag caagatcgat aaggggagta
     3601 gcttctattt cacgctccca acggccggac aatgacgctg ctgacgcagt tgttaacaag
     3661 gtgttaacac aaccttagcc tggcttttac acgcggctga cttgtcctta atagccgccg
     3721 gttatcctcc tcttagtgaa gttcgttatt ccacatgaca cccaacctgc gctggcccac
     3781 cggccgattg cgcctggttg gaaatcgcct gtgtgcgggc gaagcggtca ggggaaaaag
     3841 acaaccaatc ggaggagaaa gaatgcggag taaactactg gcgctgttga ttttggtcac
     3901 actgatggct gccgcctgtg gcggaacagc gcagcaagcg gagccggaga ctacagcccc
     3961 aacagcaacg acagttgatg agacagccgc caccgccgag ccggcagatg ccgcggagcc
     4021 aaccgcggca tctgccactt ctgccgaggg ggagggtaca atgccgctgg ccgatccgtt
     4081 ggcggtatcg ggcgacattg tgacggctgg cagttcgacc gtctacccat tggccgaggc
     4141 gatcgccgag cgctaccagg ccgagggctt cagcggccag atcaccatcg acagcattgg
     4201 cacgggcggt ggcttcgagc gcttctgcgg cacgggcgag accgacatcg ccaacgccag
     4261 ccgggcaatc aaggacgagg aagcggccaa ctgcgcggcc atcggccgga cgccggtcga
     4321 gttccgcatc ggcacggacg ggctggccgt cgtggtcagt caggagaacg acttcgtgca
     4381 agacctgacg ctggagcagc tggcgctggt gttctcgaca gcggagacgt gggcggacgt
     4441 agacccgtcg ttcccggacg agccgattga gcgctacatt cccggcaccg acagcggcac
     4501 gttcgactac ttcaccgagg tgatctaccc cgacctggaa gacggcgtgg ccaacccgca
     4561 gttggaggcg gccaacttgc agcagagcga agacgacaac gttctggtgc agggcgtggc
     4621 cggcagcccc tatgccatcg gctacttcgg ctatgcctac gcggccaata acgccgacac
     4681 ggtgcggctg gtggccgtcg agggtgttgt cccggacttc gacacagtcg agggtgggac
     4741 gtatgcgctg gcccggccgc tgttcctcta cagcgacgcg gccatcttgc aggccaagcc
     4801 gcaggtggcc gactacatca acttcttcct gaccaatgtg gacgaagaga tcgagaccgt
     4861 cggctatttc cccgccagcg atgaggccct caacgaggcc cgcaaccact ggctggtggc
     4921 tatgggcctg gccgcgacta tgcctgagaa cacgaccggc gagaccgtgg cgcagggggc
     4981 catgtgcgag ctacccgcgg ccgatccgtt ggcggtatcg ggcgacattg tgacggctgg
     5041 cagttcgacc gtctacccat tggccgaggc gatcgccgag cgctaccagg ccgagggctt
     5101 cagcggccag atcaccatcg acagcattgg cacgggcggt ggcttcgagc gcttctgcgg
     5161 cacgggcgag accgacatcg ccaacgccag ccgggcaatc aaggacgagg aagcggccaa
     5221 ctgcgcggcc atcggccgga cgccggtcga gttccgcatc ggcacggacg ggctggccgt
     5281 cgtggtcagt caggagaacg acttcgtgca agacctgacg ctggagcagc tggcgctggt
     5341 gttctcgaca gcggagacgt gggcggacgt agacccgtcg ttcccggacg agccgattga
     5401 gcgctacatt cccggcaccg acagcggcac gttcgactac ttcaccgagg tgatctaccc
     5461 cgacctggaa gacggcgtgg ccaacccgca gttggaggcg gccaacttgc agcagagcga
     5521 agacgacaac gttctggtgc agggcgtggc cggcagcccc tatgccatcg gctacttcgg
     5581 ctatgcctac gcggccaata acgccgacac ggtgcggctg gtggccgtcg agggtgttgt
     5641 cccggacttc gacacagtcg agggtgggac gtatgcgctg gcccggccgc tgttcctcta
     5701 cagcgacgcg gccatcttgc aggccaagcc gcaggtggcc gactacatca acttcttcct
     5761 gaccaatgtg gacgaagaga tcgagaccgt cggctatttc cccgccagcg atgaggccct
     5821 caatggcgcg agttgcgcgt tcgttgacgc cactggacag taacaacgac agcggtcgat
     5881 agcgccgctg ggcaaacaga cgccgcccac atctcgcgcc gcgcgggtgt gggcggcgcg
     5941 tcaccatgat cggcgctggc aacagcgccc gtaaccaacc tatccgcgcg gcgcgagcga
     6001 ttgtcatatt tgctcattcg cgcctagaat gggggtatgg agaaggaaat ggagaactta
     6061 accgtgctgc ctccgcgagg ggaggccggc gccggacggg ccggggccgc cagcgcatcc
     6121 gacccgcccg acctgagccg gcgtcaccgc atcggcgagc gcaccattga atccggtctg
     6181 ttgctcgccg ccgcagtttc catcctgacg actatcggca tcgtcgccat cttgctggaa
     6241 gaggccttgc gcttcttcgc cgatgagcgc gtcaccgtgg tcaacttcct caccgacacc
     6301 acctggcaac cacaggctag tcacttcggc attctgccgc tgctgaacgc cacgctgatg
     6361 atcaccgcta tcgccctggt cgtggccatc ccgctgggcc tggcttcggc tattttcctg
     6421 agcgagtatg cttcgccgcg ggtgcgcagc ctgctgaagc cggcgctgga gatgctggct
     6481 ggcgtgccca cggtcgtcta cggctacttc gccctgacct ttgtcacggc tatcctgcgc
     6541 gccgtcctgg gcaaggacat cgtccagatt tacaacgtcg cctcggccgg gctggtcgtc
     6601 ggcgtgctca tcacgccgct gatcgcctcg ttgagcgagg acgcgctcca cgccgtgccc
     6661 aactcgttgc gcgaagccgc ctacggcgtc ggcgcgacca agttcgagac ggcggtcaag
     6721 gtcattctgc ctgcggcgct gtccggcgta gccgcggccc tcgtcgtggc catctcgcgc
     6781 gctttcggcg agacgatggt tgtggccatc gccgccggcg cagggccaaa gctgacctgg
     6841 aaccccttcg agtcggccga gacaatgacc ggctacatgg tgcgcatcag cggcggtgac
     6901 ctgagctacg aatcgctcga ctacaagagc atctacgcta tcggcctggt tgtcttcgcc
     6961 gtcaccttgc tgcttaacat catcagccag cgcatcgtcg cccgcttccg cgaggtgtac
     7021 gaatgaacga ccatcaatcg cccgccgaga gcgccccgga gccaggcccc cagccgtccg
     7081 accgttttgt cgatctctat ccgtctgagg acggcatccg cgcccaggtg acccggcgac
     7141 agcgcgtggc cacctcctgg cgcttcgtct tcctggcggc cacctcgctg gccgtcgtca
     7201 tcctcagcac gctgctcctg tcgatcatca atcaatcctt tggtctggtg gccgaacaga
     7261 ccgatattcc cgaagcccag ctagtggccg gctattacaa gtcacagttg accggcgcga
     7321 ccaacgtcac cctctccacc gaggacgacg ccgctattgc cgccggcatc gccgcggacg
     7381 ccaacggcat tggcctgctg ggctacgccc tctacaccgc caatcagccc gcgctccagg
     7441 ccctgagcat tgacggcatc gccccatcgg ccgagaccgt caccgcgggc acgtatccgc
     7501 taacccggcc gctgctcctg tattcgtccg cggcgattct cagcgacaag ccgcaggtag
     7561 ccgccttcct cctctactac ttgcaacatg ccgaagaagt catgagcgag caaggcgcgt
     7621 ttccggccga cgctgccgcg ctggccgagc aggctgccgc cctggccacc atgctcggtc
     7681 tggacgcaac cacgcccatc gacccggccg cctacgaggg cgacatcgtt atctccggca
     7741 gttccacgct caaccccgtc acgcgcgagg tggcccggcg cttccgcagc gagggctttc
     7801 taggggcgct caagatcagc agcgtcggca ctgtcgccgg cttcgaggct ttctgtgccg
     7861 aaagcgccga cctcgttgac gccagccggc ccatcaccac agtcgagtcc gcggcctgcc
     7921 gcgccaacgg cctgacgccg ctcagcgtgc aggttggcct cgaccccatg gtcgtcgccg
     7981 tgaatagcgc caacactgct gtcaccaacg tgaccggcca gataatgcag cggccgcttg
     8041 gcgtcgccga gctgctgccg cagcgccac
//
LOCUS       DAOSMQ010000080         8027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_8969_length_8027_cov_4.860261, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000080 DAOSMQ010000000
VERSION     DAOSMQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8969_length_8027_cov_4.860261"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /gene="glgP"
                     /locus_tag="PK829_04075"
     CDS             <1..1716
                     /gene="glgP"
                     /locus_tag="PK829_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOU40429.1"
                     /translation="VGVGFFYPQGYFRQRLPSHGWQEAIYEPVYMADVPIHPVDDADG
                     RELRIEVWMGNRMVQARVWRVQVGRVPLYLIDTDVAENEPWDRELSARLYSGDSEVRI
                     RQEIMLGIGGVRLLSKLGYKPAVLHMNEGHSAFLLLESVRQEVAGGLDFETACELVKS
                     RTVFTTHTPVPAGHDAFSFSLMDKYFPGFWDELGINRDAFLSLGHHAEPWGDAFNMTV
                     LALRLSGRRNGVSQLHAEVSRQMWQPVWPDRAVADVPIMAITNGVHVPGWTAGEMRQV
                     FKEYLGADWEDHHDDPVLWERLADVPDEVLWRAHVSLKSKLMDYLRMRARHSWVAGQC
                     DPTEVLVGGTLLDPNALTIGFARRFATYKRATLLFQDMERLQRILLNMHRPVQIVFAG
                     KAHPADEPGKHLIQQVYNFAKSNYFGGRVAFVENYDLHSARYFKQGVDVWLNTPRRPR
                     EASGTSGMKASLNGIPNLSILDGWWAEGYNGANGWAVEDREFSDPDAQDAFDAETIYQ
                     LLEQEIVPLFYDRDRDGTPRGWVQVMREAIRSNAPLFSTRRMVKQYTTELYLPAMMAE
                     TEAAV"
     gene            1760..2119
                     /locus_tag="PK829_04080"
     CDS             1760..2119
                     /locus_tag="PK829_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40430.1"
                     /translation="MRVWPRRLLILLFILFWLAVMLFPTLAVVLARNGQLQLGDTDGR
                     HWRLFLLQEADFEGLGLERARPVAPPLSAPSASCTQTTVSYWLWAGEGQGANYCRCID
                     ADSGADLSDVPPACQAP"
     gene            2144..2596
                     /locus_tag="PK829_04085"
     CDS             2144..2596
                     /locus_tag="PK829_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU40431.1"
                     /translation="MSNIAVKWLEEGSRLFACRDSYGNMVLAGSWPREEPGWQEWKAA
                     KPSDLLLMSLASCAAHDVVAILERQRQRLTGLRIDVNGQQLPDPPYAFTDIHLHFTLE
                     GDELEPDKVARAVSLSVEKYCSVAATLRGVATITYSQTIATPVTPARP"
     gene            complement(2547..3059)
                     /locus_tag="PK829_04090"
     CDS             complement(2547..3059)
                     /locus_tag="PK829_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015197529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HOU40432.1"
                     /translation="MRPKPFVPAAGQESVWDYPRPPRLEPTTKHIRIVFNGVTIAETR
                     RAWRVLETSHPPVYYIPPEDIRAEVLRPAAGRSFCEWKGEAGYVTVEVDGRRAENAGW
                     YYASPTAGFAAIKGYIAFYAAPMDGCFVDGERVRPQPGSFYGGWITADVVGPFKGEPG
                     SRGWRSSGYR"
     assembly_gap    3329..3428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3429..4785
                     /locus_tag="PK829_04095"
     CDS             <3429..4785
                     /locus_tag="PK829_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40433.1"
                     /translation="QSSASTQSSASTGSSAPLLLIGWDDALGRVVRAVDPLTGRDAPG
                     YTPLTFDDRRDSDFITAHVLSADGHHLALLNTNGQLCYDFAGGETCGPRSSVVYLVDV
                     AAWTIQPVSLTPDGWVGPLAFSPDGERLALSVRGDNEVGVWLFDATNGAMLATAALPF
                     APSQLGFGPDAATVVVYGQPEGDEPGMTQPPSPRVLLLDTGTLAPVWETTLSEVSSGN
                     WCAAQCNAALDVRLMASRVPGVVLSPDGEQLLIAHADAERLTAVDLRQRTVVTVAIGK
                     AMTWLDRLLALTAGIAHAKGPTESMARYAALSPDGTRLYLGGYDFAMTAGSSGGWDVS
                     ETFTPLQVIDPITGRVVAESDAHIYWLQLSPDGAWLLRRDLSANVIATDVLDARTLTS
                     LGHIEGMDVFLARDLAGGLRFVGQSMRDRQTTFTLFDPTSFDAVARWSADGPAWLLHI
                     P"
     gene            4884..6176
                     /locus_tag="PK829_04100"
     CDS             4884..6176
                     /locus_tag="PK829_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU40434.1"
                     /translation="MTDLSALRRFSYPKTFIIGFGFLGISIIWPIFNQFVPIFLQAGN
                     PEFTQQLLAEGRAVPDVVGFGLAPSLALFIMTWDNLINIFVQPWVGARSDRTWTRFGR
                     RKPWILVGLPIAVLGFVLLPFAPTALALAVFILITNFGMALFRSPTVAWLGDLFLPDD
                     RSKANGIINLMGGIGSLLAFLGGGILFNALGRPAPFIAGAILLIVAMIVVLFGVREPE
                     QRAADADDAHSSVRDSLRVLMKDPQRSGIYVLLGILFWFIAFNALEAGLSSFAVFTLG
                     IAPGTASIYAGAISLSFLIFAWPAGIAGTRYGRRNVILVGLTGLTILFAVAYFLIQGA
                     ATFVVVLVLGGLFWAMVNVNSLPLVYDHGDERHIGAYTGLYYFSSQLAAVLGPTLGGA
                     LVDVLGNEYRWLFLFSAAFMAVAWVVVTRIRHEPAAAA"
     gene            complement(6187..6900)
                     /locus_tag="PK829_04105"
     CDS             complement(6187..6900)
                     /locus_tag="PK829_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOU40435.1"
                     /translation="MPLAQITHADTTIYWLCEDESAATNPALLSPAEAAAYAALRAGK
                     RRRDWLLGRRVAKRLVQASMAARLGQTPPLSEITVLSHADGWPQVSLPAALATGPITL
                     SISHSHDRALCALVWGNQPLGCDLEFIEPRSAGFVADYFTAAERQYLAAATPADHPTL
                     ANAIWSGKEAALKAIRRGLAEDTRLVSCLPGEAADADGWRSLRYRWEEPARALPPLTG
                     WWRAQNGFVMTLAAGRAES"
     gene            complement(7059..>8027)
                     /locus_tag="PK829_04110"
     CDS             complement(7059..>8027)
                     /locus_tag="PK829_04110"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HOU40436.1"
                     /translation="VGGRLGILASEWDATGGLDLAKANAWLAARQPQLLMLGHITAAK
                     LYGGLEVETTLDPKEQPFLYDHAMEGTPLLPGVMGTESFGQLALALAPGYRVAAIFDE
                     QFHAPFKFYRMEPQTLYLSGFATPAADGNLVAHATLRSVRDLGKPGLPPQQKVHFTAS
                     VLLARELDRPVLDFTPPAAEALPIPADAIYRVYFHGPAYQVLERAAVTGETAIGLMTA
                     DLPANTSPADVASVMAPRLIELCFQTAGVWDIQTTGTMALPLGLASVTAYRQPTEADG
                     RRLYALVEAIDGGDAYHARVLDEAGHVYVELKGYRTVDLPGSVSLS"
BASE COUNT         1203 a         2603 c         2668 g         1453 t
ORIGIN      
        1 gtcggcgtgg gcttcttcta tccgcagggc tacttccgcc agcgcctgcc ctcccatggc
       61 tggcaggagg cgatttacga gccggtctac atggccgacg tgcctatcca tccggtggac
      121 gacgccgacg gccgcgaact gcgcatcgag gtgtggatgg gcaaccgcat ggtccaggcg
      181 cgcgtctggc gggtgcaggt cggccgcgtg ccgctctatc tcatcgacac cgatgtggcc
      241 gaaaatgagc cgtgggatcg cgagctgtcg gcccgcctct attccggtga cagcgaggta
      301 cgcattcgcc aggagatcat gctgggcatt ggcggcgtgc ggctgctgag caagctgggc
      361 tacaagccgg ccgtgctgca catgaacgag ggccactccg ccttcctgct gctggagagt
      421 gtgcgccagg aagtggccgg cgggctggac ttcgagacgg cctgtgagct ggtcaagtcg
      481 cgcactgtct tcacgaccca cacgcccgtg cccgcgggcc acgacgcctt ttccttctcg
      541 ctgatggaca agtatttccc cggcttctgg gacgagttgg gcatcaaccg cgatgcgttt
      601 ctcagcctgg gccaccacgc cgaaccgtgg ggcgacgcct tcaatatgac cgttctggcg
      661 ctgcggctct ccgggcggcg caatggtgtc agccagctgc atgccgaagt ctcgcgccag
      721 atgtggcagc ccgtctggcc cgatcgggcg gtggccgatg tgccgatcat ggccattacc
      781 aacggcgtcc acgtgcccgg ctggacggcc ggcgagatgc gccaggtgtt caaggagtac
      841 cttggcgccg actgggagga tcaccacgac gaccctgtgc tctgggagcg gctggccgat
      901 gtgcccgacg aggtgctgtg gcgcgcccat gtcagtctga agagcaagtt gatggactac
      961 ctgcgtatgc gcgcccgcca ttcctgggtg gccgggcagt gcgacccgac cgaggtgctt
     1021 gtcggcggca cgctgctgga tcccaacgcg ctgaccatcg gctttgcgcg ccgctttgcc
     1081 acctacaagc gggcgacgct gctgttccag gacatggagc ggttgcagcg catcttgcta
     1141 aacatgcacc ggccggtgca gatcgtcttc gccggcaagg cccacccggc cgacgagccg
     1201 ggcaagcacc ttatccagca ggtctataat ttcgccaagt ccaactactt cggcgggcgc
     1261 gtggcctttg tcgagaacta cgacctgcac tcggcccgct atttcaagca gggcgtcgac
     1321 gtctggctga acacgccgcg ccgcccgcgc gaggccagcg gcaccagcgg catgaaggca
     1381 tcgctgaacg gcatccccaa cctgagcatc ctcgatggct ggtgggccga gggctacaat
     1441 ggcgccaatg gctgggcggt ggaggatcgc gagttcagcg accccgacgc gcaggacgcc
     1501 ttcgacgcgg agacgatcta ccagctactg gagcaggaga tcgtgcccct cttctatgac
     1561 cgcgaccgcg acggcacgcc acgcggctgg gtacaagtca tgcgcgaggc cattcgctcc
     1621 aacgcgccgc tgttcagcac gcggcgaatg gtgaagcaat atacgaccga gctatacttg
     1681 ccggcgatga tggccgaaac ggaagcggcc gtctagcgcc ggcggtgttg ccggccgacc
     1741 cgtattgccg agagaattta tgagagtgtg gccgcgccgg ttgctgattt tgctgttcat
     1801 tctgttctgg ctggcggtga tgctgttccc gacgctggcc gttgtgctgg cgcgcaacgg
     1861 ccagttgcaa ctgggcgaca ccgatgggcg acattggcgg ctgttcctgt tgcaggaggc
     1921 cgattttgag gggctggggc tggagcgggc gcgccccgtc gccccgccgc tgagcgcgcc
     1981 gtcggctagt tgtacccaga cgacggtgtc ctactggctc tgggcaggcg aggggcaggg
     2041 agccaactac tgccgctgca tcgacgccga cagtggcgcc gacctgtccg acgtgccgcc
     2101 cgcctgccaa gcgccatagc gctatgcccg taaggaggtt ctgttgagca acatcgccgt
     2161 caagtggctg gaagagggca gccgcctctt cgcctgtcgc gattcctacg gcaatatggt
     2221 tctggccggc tcctggccac gggaagaacc cggctggcag gagtggaagg cggccaagcc
     2281 gtccgatcta ctgttgatga gcctggcctc gtgcgcggcc cacgatgtcg ttgccatcct
     2341 ggagcgacag cggcagcggt taaccgggct gcgtattgac gtgaacggcc aacaattgcc
     2401 cgacccgccc tatgccttca ccgacatcca cctgcacttc acgctggagg gtgacgagct
     2461 ggaacccgac aaggtggccc gcgctgtgag cctgtccgtc gagaagtact gctcggtggc
     2521 ggccaccctg cgcggcgtgg ccacgattac ctatagccag acgatcgcca cccccgtgac
     2581 cccggctcgc ccttaaaggg gccgaccacg tcagcggtga tccagccgcc atagaagctg
     2641 cccggctggg ggcggacacg ctcgccgtcc acaaagcagc cgtccatagg cgcggcgtag
     2701 aaggcgatat agcccttgat ggcggcgaag ccggccgtcg gactggcgta ataccagccg
     2761 gcgttctcgg cgcggcggcc gtctacttcg acggtgacgt agcccgcttc gcctttccat
     2821 tcacagaagg agcggcccgc ggccggccgc agaacttcgg cgcgaatgtc ctctggcggg
     2881 atgtagtaga ccggcgggtg gctcgtctcc aggacacgcc acgcccggcg cgtctcggcg
     2941 atggtcacgc cgttgaagac gatgcggatg tgtttcgtcg tgggttcaag gcggggtggg
     3001 cgggggtagt cccagacaga ttcctggccg gcggcgggta caaacggttt aggtcgcata
     3061 gcgtcttcct catgctctta acgtgaagcc gattatagac atagttgtgg caagggtcgc
     3121 atcagccttt ccttgcccac gtcgccaacc gaccgcgcaa tcactccctg agggaaccaa
     3181 tcccccgctg gtggtgtact ccctcatatg aatacgcagg tacgaggttg gacggtgaaa
     3241 caattaacct ggctggcgtc gttgttgcta ctggcggcct gtgccgcgcc gctggagtcg
     3301 tctgcgtcca cgcagtcgtc tgcgtccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnngc agtcgtctgc gtccacgcag tcgtctgcgt ccaccggttc gtccgctccg
     3481 ctgctgctca tcggctggga tgacgcgctc ggccgcgttg tccgcgccgt cgatccactg
     3541 accggccggg acgcgcccgg ctataccccg ctgacgttcg acgaccggcg cgacagcgac
     3601 ttcatcaccg cccacgttct atccgccgat ggccaccacc tggcccttct gaacaccaac
     3661 gggcagctct gttatgactt cgccggcggc gagacttgtg gtccgcggtc gtcggtcgtc
     3721 tacctggtcg atgttgccgc ctggaccata cagccggtct cgctgacgcc tgacggctgg
     3781 gtcggcccgc tagccttcag ccccgacggc gagcgcctgg ctttgtccgt tcgcggcgac
     3841 aatgaggtcg gtgtgtggct gttcgacgcc accaacgggg cgatgctggc cacagccgcc
     3901 ctgcccttcg cgccatcgca gctcggcttc ggcccggacg cggcgactgt agtcgtctac
     3961 ggccagccgg agggcgatga gccaggcatg acccagccac cgtcgccgcg cgtgctccta
     4021 ttggacaccg gcacgctcgc gccggtatgg gagaccactc tgagcgaggt cagcagcggc
     4081 aattggtgtg ccgcgcagtg caacgccgcg ctcgacgtca ggctgatggc ctcgcgcgtg
     4141 ccgggcgttg tgctgtcgcc ggacggggag cagctgctca ttgcccacgc cgatgctgag
     4201 cggctgaccg cggtcgatct gcggcaacgc accgtcgtga cggtggccat tggtaaggcc
     4261 atgacctggc tcgaccggct cctggcactc acagccggca ttgcccacgc caagggcccg
     4321 accgagagca tggctcgcta cgcagccctc tcacccgacg ggacgcggct ctacctgggc
     4381 ggctacgact tcgccatgac agccggtagc agcggcggct gggacgtgag cgagacgttc
     4441 acgcccctgc aagtgattga cccgatcaca ggccgtgtgg ttgcggagag cgacgcccac
     4501 atttactggc tgcaattgtc acctgacggc gcgtggctac tgcggcgcga tctgtctgcc
     4561 aacgttattg ccaccgatgt gctcgatgcg cggacgctga cttccctggg ccacatcgag
     4621 ggcatggatg tgtttctagc acgcgatctg gccggcggcc tgcgctttgt cggccagtcc
     4681 atgcgcgatc gccagacgac cttcacgctc ttcgatccga ccagcttcga cgctgtggcc
     4741 cggtggtcgg ccgacggccc ggcgtggctg ctgcatatcc cttaggccgc gccgccattc
     4801 gttgcccatc ctgcccgtca cctctatact ggcgttgcgt ctccccgggc gcggcgactt
     4861 caacatcatc acgggaggca acggtgacag acctgtcggc gctgcggcgc ttctcctatc
     4921 ccaagacgtt catcatcggc ttcggattcc tgggcatcag catcatttgg cccatcttca
     4981 atcagttcgt gcccatcttc ttgcaggcgg gcaaccccga gttcacccaa cagttgttgg
     5041 ccgaggggcg ggccgtgccc gacgtggtcg gcttcggcct ggccccgtcg ctggcgctgt
     5101 tcatcatgac ctgggacaac ctgatcaaca tcttcgtcca gccctgggtt ggcgcgcgca
     5161 gcgaccggac gtggacgcgc ttcggccggc gcaagccgtg gattctggtc ggcctgccca
     5221 ttgccgtgct cggcttcgtg ctgctgccct tcgcgcccac ggcgctggcc ctggccgtct
     5281 tcattctcat caccaacttt ggtatggctc tcttccgctc gccgacggtg gcctggctgg
     5341 gcgacctgtt cctgcccgac gaccgcagta aggccaacgg catcatcaac ctgatgggcg
     5401 gcatcggcag cttgctcgcc ttcctgggtg gcggcatcct gttcaacgcc ctcggccggc
     5461 cggcgccctt tattgccgga gcaatcttgc tcatcgtggc catgatcgtc gttctgttcg
     5521 gcgtgcgcga gccggagcag cgcgccgccg atgccgacga cgcccacagc tccgtgcgcg
     5581 acagcctgcg cgtcctgatg aaagatccgc agcgcagtgg catctacgtg cttctgggca
     5641 tcctcttctg gttcatcgcc tttaacgcgc tggaggcggg gttgtcgtcc ttcgccgtgt
     5701 tcaccctggg cattgccccc ggcacggcgt cgatctacgc cggggcgatt agcctgtcgt
     5761 tcctcatctt cgcctggccg gcgggcattg ccggtacgcg ctacggccgc cgcaacgtca
     5821 tcctcgttgg cctgaccggt ctgacgatcc tgttcgccgt ggcttacttc ctcattcagg
     5881 gcgcggcgac cttcgtcgtc gtgctggtgc tcggcggctt gttctgggcg atggtcaacg
     5941 tcaacagcct gccgctggtc tacgaccacg gcgacgagcg ccacatcgga gcctataccg
     6001 gtctgtacta cttctcgtcg cagctggctg ccgttctcgg tccgacgctg ggcggggcgt
     6061 tggtggacgt gctcggcaac gaatatcgct ggctgtttct gttcagcgcg gcgttcatgg
     6121 ccgtggcctg ggtggtggtg acgcgcatcc gccacgagcc ggcggcggcg gcatgagcga
     6181 cggcgctcaa gactccgcgc gaccggcggc cagcgtcatg acaaagccgt tctgcgcccg
     6241 ccaccaaccc gtcagcggcg gcagcgcgcg cgccggctct tcccagcgat agcggagcga
     6301 gcgccagcca tcggcatcag ccgcttctcc tggcaggcag gacaccagcc gcgtatcctc
     6361 cgccaacccc cggcgaatgg cctttagcgc cgcttccttg ccgctccaga tggcgttggc
     6421 cagggtgggg tggtcggccg gcgtggcggc ggccaggtat tggcgctcgg cggcggtgaa
     6481 gtagtcggcc acgaagccgg cggagcgcgg ctcgatgaat tccaggtcgc agccgagggg
     6541 ctggttgccc cagacgaggg cgcagagagc gcggtcgtgg ctgtggctga tcgacagagt
     6601 gatcggcccg gtggcgagtg ccgccggcag cgacacctgc ggccaaccgt cggcgtggct
     6661 gaggacggtg atctcggaaa ggggtggcgt ttggccgagg cgggcagcca tgctggcctg
     6721 cacgagacgc ttggcgacgc gccggccgag gagccagtcg cggcgtcgct tgccggcgcg
     6781 tagcgcggcg taggcggcgg cctctgcggg ggagagcagc gcgggattcg tcgccgccga
     6841 ttcgtcttcg catagccaat agatggtggt gtctgcgtgg gtgatctgcg ccagtggcat
     6901 cattgtccgc gccacggttg tcccgtcagt cgttgcgccc ctaccgtgcc gggcgttatt
     6961 ccagtgacca taaagctatt atacgacagc aaaccgcccg ctggatcaga ggaatccagc
     7021 gggcggttgc cacaggttag tgaggaggta actgcgagct atgacaggct aacgctgccg
     7081 ggcagatcaa ccgtgcgata cccctttagc tcaacgtaga cgtggccggc ttcgtcgagc
     7141 acgcgggcgt ggtaagcgtc gccgccgtcg atggcttcga caagggcata gagccgccgg
     7201 ccgtcggctt cggtgggctg gcgataggcg gtcaccgacg ccaggcccag cggcagggcc
     7261 attgtgcccg tcgtctggat gtcccacacg ccggctgtct ggaagcacag ttcgatcagc
     7321 cgcggggcca tgaccgaagc cacgtcggcc gggctggtgt tggccggcag gtcggcggtc
     7381 atcaggccga tggcggtctc gccggtcacg gccgcccgct ccaacacctg gtaggccggg
     7441 ccgtggaagt agacgcggta gatggcgtcg gcggggatgg gcagcgcctc ggccgcgggc
     7501 ggggtgaagt cgaggactgg cctatccagt tcgcgggcca gcaggacgct ggccgtgaag
     7561 tggacctttt gctgtggcgg cagaccgggc ttgcccaggt cgcgcaccga gcgcagggtc
     7621 gcgtgggcga cgaggttgcc gtcggccgcc ggtgtcgcga agccgctcag gtagagcgtc
     7681 tgcggctcca tgcggtagaa cttaaacggc gcgtggaact gctcgtcgaa gatagcagcg
     7741 acgcggtagc caggggccag cgccagcgct agttgtccga acgactccgt gcccatgacg
     7801 ccgggcagta gcggcgtgcc ctccatggcg tggtcgtaga ggaacggctg ctccttggga
     7861 tcgagcgtcg tctccacttc caggccgcca tagagcttgg ccgccgtgat gtggccgagc
     7921 attagcagct gcggctgccg cgccgccagc caggcattgg ccttagcgag gtcgaggccg
     7981 ccggtagcgt cccactccga ggccaggatg cccagccggc cgccgac
//
LOCUS       DAOSMQ010000081         7998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9011_length_7998_cov_4.376180, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000081 DAOSMQ010000000
VERSION     DAOSMQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9011_length_7998_cov_4.376180"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PK829_04115"
     CDS             <1..562
                     /locus_tag="PK829_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOU40437.1"
                     /translation="CGLALDVAVAAGLRVGQTISAADLTALEQREEVHHARERAVALL
                     ARRPRSANEIARQLRRHQHADDIIQQVLDDLTAAGLLDDNAFAAYWVEQRDAFRPRSR
                     LALRQELSQKGLDREVVSEALEGLDEIEAARRVARKQAGRWRTLPEAEWRAKMTRYLL
                     RHGYPYDVVGEVVTEIWPEIKPDDET"
     gene            577..2109
                     /gene="rny"
                     /locus_tag="PK829_04120"
     CDS             577..2109
                     /gene="rny"
                     /locus_tag="PK829_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOU40438.1"
                     /translation="MELWIGLIIGLLLGALAAALIMRSVMRPKIDALVAATEAELAEK
                     RLTAEREADNILRVSREEAQELRTEAEKAIERRYQDLARAEERVDRRSANQDSQAKKL
                     ELREQVLNKRQSKIDKRQNELADLETVHREKLEEIAAMTTDEARVVLLQQVEREAQQD
                     LARVMREIDNRAKETAEAQARKIIALAIQRLASDQVAEMVVSTVPLPSDEMKGRIIGR
                     NGRNIRAFEQAAGVDIIVDDTPEAVTVSSFDPVRREVARRALAKLITDGRIHPARIEK
                     LISDATEEVERDIKEAGEQAAYEANVHGLHPEILKTLGRLKYRTSYGQNQLHHAVEAS
                     HIAALLAAELGANIETAKMGALLHDLGKAMDHDQEGTHAQIGAEFAKRFKVPPVVVNA
                     IASHHHEVDQDTVEAIIVEAADAISGARPGARRESLENYIKRVRTLEEIATSFAGVES
                     AYALQAGREIRILVKPNQIDDLAAVRLSKEISKNIEDSMQYPGQIQVTVIRETRAVGF
                     AK"
     gene            2219..3070
                     /locus_tag="PK829_04125"
     CDS             2219..3070
                     /locus_tag="PK829_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOU40439.1"
                     /translation="MLTTIAIIGSGTMGRGIAQSAALAGYGVRLYDLTEEIAGQGLRT
                     IAASIEEGVRRGKTRPETAAQAVAALSPTSDLAVAARADLVIEAVPEDIALKRQLFGH
                     LDAAAPPHTVLASNTSSLSISALAAATGRPDRVLGLHYFNPAHLMPLVEIIRGDHTSD
                     ATVALAHDFVERLGKTPVLCQDSPGFIVNRVARPFYGEAFRLLGEHAADVETIDRLIR
                     SLGFRMGPFELLDLIGLDVNLAVTQAVYNAYFQDPKYRPHPLQQRMVDSGRLGRKSGH
                     GFYDYER"
     gene            3295..4374
                     /locus_tag="PK829_04130"
     CDS             3295..4374
                     /locus_tag="PK829_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU40440.1"
                     /translation="MEQTTSKGQWQLWVGLAVGLACLAIVFIFFAKPGDIIDNLRHVH
                     LGYLLLGALGVILFLVIRAVRWRFMLSGAGATVDGATVHDAGGPAAPPYAQVFHIQNI
                     GYMLTQLLPLRLGDVARAILIGNVPPVTIARGLSTMVVERLLDMLFIVVLLPFTLSGI
                     PSLPGWMRSFALFSGFAALAGIVVLIVAANQRARAVRLAGWVLGRLPFLDRAAWVRRA
                     DELLLGLKSLTSLRDGAVLLVLSVIVWLPVLAAYFFTLRAVGLTPTLALTGFVVCAAA
                     FSVAVPSTPGQAGPYHVAVIAALQLAGQPATASASFAFLYHIMNIIVMVLMGLWGIAS
                     TGVTFRNVLDSARSFATRRHTESAP"
     gene            4554..5789
                     /locus_tag="PK829_04135"
     CDS             4554..5789
                     /locus_tag="PK829_04135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU40441.1"
                     /translation="MKILEVLTYYRPWVSGLTIYVERLSRALVAQGHDVTVLTAQYDP
                     ALPRYDVMDGVKIVRIPVALRVSKGVLMPTFGPMAWRLAREADVLHLHLPQFDAPGLA
                     TRGRLLGKPVVLTYHCDLQLPQGGFNRLVDRVVRLQNDTAGRLADAVVTYTRDYASHS
                     PYLSQYIDRKLAIIPPPIDLDEASEAVVRAFAAQHHLGERPIIGISARLAAEKGVEVL
                     LRALPRVLERFPDALVLHAGPYKDILGEEAYAARLAPLFEQYAGHYRLLGTLNGAELT
                     AFYRNLDVLCICSLNSTESFGLVQIEAMRNGVPVAACALPGVRQPVTMTGMGEVTPVG
                     DSDALAEALIRLLSDAGRYRRPAQTIADSFSPAQTAAEYLRLFEALQRGERLGLTAEP
                     PAYERLRALSAEPATAAQP"
     gene            5822..6697
                     /locus_tag="PK829_04140"
     CDS             5822..6697
                     /locus_tag="PK829_04140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU40442.1"
                     /translation="MTNDDLLWQQLKSLPAFRALLRAVEARFYPLIPLPEPTLDVGCG
                     DGHFAAMTFPDRTLAAGIDPWWGPLHKAVRSGKYALPIQALGDRLPFADGTFASAFSN
                     SVLEHIPDLPPVLAEVNRVLRPGARFVITTPSHLFTEYLGGAAFLERLRLGGLAQRYR
                     RLFNAISRHAHTDSPETWAARLAEAGFAVERWQYYFSRGALRALEIGHAQGLPSAALH
                     ALTGHWILAPWESSLAPTERWVRPFYEEEAAPDGGAYLLFIVRKQASGPIAAELPPPR
                     PLPVRRAAAEPAPSA"
     gene            6747..7925
                     /locus_tag="PK829_04145"
     CDS             6747..7925
                     /locus_tag="PK829_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU40443.1"
                     /translation="MMPEQPLLPNNSPLWSRTTKTIVVVTVLLLAALLVMRFRTLLVM
                     VTMAAILAYLLDPLISFIDRRTSIKRGIIIAVVYVLLAAALVGGFFALGVASYQQALN
                     LIDEVPGLVENVARLIATLVNSREPITIGPLSLDPSTVAWDRLPEQLLGMVEPLVTQS
                     GGVLSRLATSTARTVFNLFFIFVLSIYLASDLPRFGGYVKSFAQRPGYREDAEQLLPR
                     LRHVWSAYLRGQIVLSLVIFLTVWIGLTLLGVQNSLALGLLAGLLEFLPNLGPVISAV
                     VTILVAFFQPGNYLGLASWQYTLVVLALMIVIQQLENHLLVPRIVGGALNLHPIIVIV
                     GLFIGASLGGILGAILAAPLIASIKVLSQYAWRKLFDLPPFPVDEPLDDPPPHPFHPE
                     "
BASE COUNT         1324 a         2798 c         2518 g         1358 t
ORIGIN      
        1 ctgcgggctg gcgcttgacg tggccgtggc cgcggggttg cgtgtcggcc agacaatcag
       61 cgccgccgat ctgaccgcgc tggagcagcg cgaggaagtc caccacgccc gcgagcgagc
      121 tgtggctctc ttggcccgcc ggccgcgcag cgccaacgag atcgcccgcc aattgcgccg
      181 ccatcaacat gctgacgaca tcatccagca agtcctcgac gatctgaccg ccgcgggtct
      241 gctcgacgac aacgctttcg ccgcctattg ggtcgaacag cgcgatgcct tccgcccgcg
      301 cagccggctg gcgctgcgcc aggagctgag ccaaaaagga ctagaccgcg aagtcgtcag
      361 cgaagcactg gaaggactgg acgagatcga agccgcgcga cgtgtcgcgc gtaaacaagc
      421 cggccgatgg cgcactctgc ccgaagcgga gtggcgcgcc aagatgaccc gctatctgct
      481 gcgtcatggc tacccctatg acgtcgtcgg cgaggttgtc actgaaattt ggccggagat
      541 caaacctgac gacgaaacct agaggagata gcaatcatgg aactatggat agggctgatc
      601 atcggcctgc tgctgggcgc gctggccgcg gcgctcatca tgcggtcggt catgcgcccc
      661 aaaatcgatg ccctggtagc ggcgacagag gccgaattgg ccgagaaacg cctgaccgcc
      721 gagcgagagg ccgacaacat cctgcgtgtc tcgcgtgagg aagcccagga actgcgcacc
      781 gaggctgaga aggctattga gcggcgctat caggacctgg cccgcgccga ggagcgcgtc
      841 gaccggcgca gcgccaacca ggacagccag gccaaaaagc tggagttgcg cgagcaggtg
      901 ctgaacaagc gccagagcaa gatcgacaag cgccagaacg aactggccga cctggagaca
      961 gtccaccgcg agaagctgga agagatcgcc gccatgacca ctgacgaggc gcgcgtcgtc
     1021 ctgctgcaac aggtggagcg cgaggcccag caagacctgg cccgcgtcat gcgcgagatc
     1081 gacaaccgcg ccaaagaaac ggccgaagcc caggcgcgca agatcatcgc cctggccatc
     1141 cagcgattgg ccagcgacca ggtggccgaa atggtcgtct caactgtgcc gctgcccagt
     1201 gacgagatga aggggcgcat catcggccgt aacggccgca acatccgcgc ctttgagcag
     1261 gccgccggcg tcgatatcat cgtcgatgac acgccggaag cagtcacggt gtcgagtttc
     1321 gatccagtgc ggcgcgaggt cgcccggcgg gcgctggcca agctcattac cgatgggcgc
     1381 atccacccgg cgcgcatcga gaagctcatt agcgacgcca ccgaggaagt cgagcgcgac
     1441 atcaaagaag cgggcgagca ggccgcctac gaggccaacg tccatggcct gcacccggag
     1501 atcctcaaga cgctcggccg cctgaagtac cgcacctcct atggccagaa ccagctccat
     1561 cacgccgtcg aggcgtccca cattgccgcc ctgttggccg ccgaactagg agccaacatc
     1621 gagacggcca agatgggcgc gctgcttcac gatctgggta aggccatgga ccacgaccag
     1681 gaaggcaccc acgctcagat cggcgcggag tttgccaagc gcttcaaagt gccgcccgtc
     1741 gtcgtcaacg ccatcgcctc gcaccaccac gaagtcgatc aggacacggt cgaggccatc
     1801 atcgtcgagg ccgccgacgc catctccggc gcgcggccgg gggcgcggcg cgaaagcctg
     1861 gagaactaca tcaagcgcgt ccgcacgctg gaggaaatcg ccacgtcctt cgccggcgtc
     1921 gagagcgcct acgccctgca agcggggcgc gagatccgca tcctcgtcaa gcccaaccag
     1981 atcgacgacc tggccgccgt gcgcctgtct aaggagattt ccaagaacat cgaagacagc
     2041 atgcaatacc ccggccagat tcaggtgacg gtcatccgcg agacgcgggc cgtggggttt
     2101 gcgaagtagg gcagcaagta atgtaataga agcgggggag caggggggaa agaatctccc
     2161 ccgctcccct gctctcccgc tccctgcccg ctcccccgca cgacaacgaa caagaaccat
     2221 gctcaccacc atcgcgatca tcggctccgg caccatgggg cggggcatcg cccagtcggc
     2281 ggcgctggcc ggctatgggg tacggctcta cgatctgacc gaggagatcg ccggccaggg
     2341 gctgcggacg attgccgcca gcatcgagga gggcgtgagg cgcggcaaga cgcgaccgga
     2401 gacggccgcg caggccgtgg ccgccctctc gccaacgagc gacctggccg tggccgcccg
     2461 cgccgacctg gtcatcgagg ccgtgccaga ggacatcgcc ttgaagcgcc aactcttcgg
     2521 ccacctggac gccgccgccc cgccccacac cgtgctggcc agcaacacca gcagcctcag
     2581 catcagcgcc ctggccgcgg ccaccggccg gcccgaccgc gttctgggcc tgcactattt
     2641 caaccccgcc cacctcatgc cgctggtgga gattatccgc ggcgaccaca ccagcgacgc
     2701 caccgtcgcc ctggcccacg acttcgtgga gcgactgggc aagacgccag tgctgtgcca
     2761 ggactcaccc ggcttcatcg tcaaccgtgt cgcccgcccc ttctatggcg aggcgttccg
     2821 gctgctgggc gagcatgccg ccgatgtcga aaccatcgac cggctcattc gctcgctggg
     2881 cttccgcatg gggccgttcg agttgctcga cctcatcggg ctggatgtga acctggccgt
     2941 gacgcaggcg gtctacaacg cctacttcca ggaccccaag tatcggccgc acccgctcca
     3001 gcagcgcatg gtcgatagcg gccggctggg gcgcaagagc gggcacggct tctatgacta
     3061 cgagcgctag ccggcgcgac cgaggttctt agctgttggc catcccacgc aggtctgacg
     3121 ggtaggcggt tgacggtatc tgacggcaac aaggcctggg gcggccagtc gttctcggct
     3181 ggcagttcca ttcgtggacg ctcaactgtc aggtcggtgc gccggggatg actttggcaa
     3241 agccctggaa agtgaataag ccgtctaaag aaccactgat ataatagacc ctctatggag
     3301 caaacaacca gcaagggaca atggcagctt tgggtggggt tggcagtggg cttggcctgc
     3361 ctggccatcg tcttcatttt cttcgccaag ccaggcgaca tcatcgacaa cctgcgccat
     3421 gtccacctcg gctacctgct gctgggcgcg ctgggcgtca tcctctttct ggtcatccgc
     3481 gccgtgcgct ggcgcttcat gcttagcggt gccggtgcca ccgtagacgg tgccaccgtc
     3541 cacgacgccg gcggcccggc cgcgccgccc tatgcccagg tcttccacat ccagaacatc
     3601 ggctacatgc tgacccagct tctgccgctg cgcctcggcg acgtggcccg cgccatcctc
     3661 atcggcaacg tcccgccggt gaccatcgcc cgcggcctgt cgacgatggt cgtcgaacgg
     3721 ctgttggata tgctcttcat cgtcgtcctg ctgccgttca ccctgtcggg cattccctcg
     3781 ctgcccggct ggatgcgctc ctttgccctg ttctccggct tcgctgccct ggccggcatt
     3841 gtcgtcctga tcgtcgctgc caaccagcgg gcgcgggccg tgcgtctggc cggctgggtg
     3901 ctgggccgtc tgccattcct ggatcgcgcg gcctgggtgc ggcgggccga cgaattgctg
     3961 ctgggcctga aaagcctgac cagcctgcgc gacggggcgg tgctgctcgt cctgagcgtg
     4021 atcgtctggc tgccggtgct ggcggcttac ttcttcactc tgcgcgccgt gggcctgaca
     4081 ccgacgctgg ccctgaccgg cttcgtcgtc tgcgccgcgg ccttcagtgt cgccgtgcca
     4141 tccacgcccg gccaggccgg cccctaccac gtggccgtca tcgccgcgtt gcaactggcc
     4201 ggacagccgg ccacggcctc ggccagcttc gcctttctct accacatcat gaacatcatc
     4261 gtcatggtgc tcatgggcct gtggggcatc gccagcacgg gcgtcacctt tcgcaatgtt
     4321 ctcgactcag cgcgctcctt cgccacccgc cggcacacag agtctgctcc ctgagggcaa
     4381 ggcctggcca gcgtgtataa gaatatggcg gtgttgcacc gccgtattgc accacggtgg
     4441 tgcaccacgg ccatgactgt caacgtgatc agcgccgggt gtcgtcccgt caggaggggt
     4501 ccgtcccact aaccgcgtgg ccatgcccgg cgcccaactg ctagacgctt gctatgaaga
     4561 ttctggaagt cctgacttac tatcgcccct gggtcagcgg tctgaccatc tacgtcgagc
     4621 ggcttagccg cgcgctggtc gcccaggggc acgacgtgac cgtactgact gcgcagtacg
     4681 acccggccct gccacgctat gacgtcatgg acggcgtcaa gatcgtgcgc atccccgtgg
     4741 ccctgcgcgt tagcaagggc gtgctgatgc ccaccttcgg gccaatggcc tggcggctgg
     4801 cgcgtgaggc cgacgtgctg cacctgcacc tgccgcagtt cgacgcgccc ggcctggcca
     4861 cgcgcggccg gctgctgggc aagcccgttg tgctgaccta ccactgcgac ctgcaactgc
     4921 cccagggcgg cttcaatcgt ctcgtcgacc gcgtggtgcg cctgcaaaac gacaccgccg
     4981 ggcggctggc tgatgccgtt gtcacctaca cgcgcgacta cgccagtcac tcgccctact
     5041 tgtcgcagta tatcgaccgc aagctggcca tcattccccc gcccatcgac ctggacgagg
     5101 ccagcgaggc ggtggtgcgc gccttcgccg cgcagcatca cctgggcgag cggccgatca
     5161 tcggcatctc cgcgcgactg gcggccgaaa agggcgtcga ggtgctgctg cgggcgctgc
     5221 cgcgcgtcct ggagcggttt cccgacgccc tggtgcttca cgccggccct tacaaagaca
     5281 tcctcggcga ggaagcctac gccgcgcgac tggccccttt atttgagcaa tacgccggcc
     5341 actaccgcct gctgggcaca ctgaacggcg cggaactgac cgccttctat cgcaatctcg
     5401 acgtgctgtg catttgcagc ctcaacagca cggagagctt cggcctggtg cagatcgagg
     5461 cgatgcgcaa cggcgtgccc gtagccgcct gcgccctgcc cggcgtgcgc cagccggtta
     5521 caatgacggg catgggcgaa gtcacgcctg tcggtgacag tgacgcgctg gccgaggcgc
     5581 tcattcgcct ccttagcgac gccggtcgct atcgccgccc ggcgcaaacc attgccgaca
     5641 gcttctcgcc ggcccagacg gcggctgagt acctgcgcct ttttgaggcg ttgcagcgtg
     5701 gcgagcgcct gggcctgacg gccgagccgc cagcctatga acgcttgcgt gccctgagcg
     5761 ccgaaccggc gacggccgca cagccatgac caccccaccg ccaaccctga acgagcgacc
     5821 cgtgacgaat gacgatctcc tctggcaaca actcaagtcc ttgccggcct tccgcgccct
     5881 gttgcgggcc gtcgaggccc gcttctaccc gctcatcccc ctgccggagc cgaccctcga
     5941 cgtgggctgc ggcgacggcc acttcgcggc gatgaccttt cccgaccgca ccctcgccgc
     6001 gggcatcgac ccgtggtggg ggccgctgca caaggccgtc cgcagcggca agtacgccct
     6061 gcccatccag gccctgggcg accgcttgcc ctttgccgat ggcacgttcg ccagcgcttt
     6121 cagcaactcg gtgctggagc acatccccga cctgccgccg gtgctggccg aggtcaaccg
     6181 ggtgttgcgg ccgggggcgc gcttcgtcat cacgacgccc agccatcttt tcaccgagta
     6241 cctgggcggg gcggcgttct tggagcgcct gcggctgggc ggcctggcgc agcgctaccg
     6301 gcggctattc aacgccatct cccgccatgc ccacaccgat tcgccggaga cgtgggcggc
     6361 gcggctggcc gaggcaggct ttgctgtgga gcgctggcag tactacttct cgcgtggggc
     6421 gctgcgggcg ctggagatcg gccacgcaca ggggctgccc tcggcggcgc tgcacgccct
     6481 gacgggccac tggattctag cgccgtggga gagcagtctg gccccgacgg agcgttgggt
     6541 gcggccgttc tatgaggagg aggccgcgcc agacggcggg gcctacctgc tgttcatcgt
     6601 tcgcaagcaa gcgtccggcc ccatcgccgc cgagttgccg ccgccgcggc cgctgcccgt
     6661 caggcgcgcc gcggccgaac ccgcgccgtc cgcctgacaa acgcccggca acggcgtata
     6721 cttgcagcgg ttcacggaac ctcgccatga tgccggaaca gccgctcttg cccaacaact
     6781 cgccgctgtg gagccgcacc accaagacca ttgtggtcgt aacggtgttg ctgctggcgg
     6841 ctttgctggt catgcgcttc cggacgctgc tggttatggt gacgatggcc gctattctgg
     6901 cctacctgct cgacccgctg atcagcttca tcgaccggcg caccagcatc aagcgcggca
     6961 tcatcatcgc cgtcgtctac gttctgttgg cggcggcgct ggtgggcggg ttctttgccc
     7021 tgggagtggc ctcctaccaa caggcactga acctcatcga cgaagtgccc ggcctggtgg
     7081 agaacgtcgc ccggttaatc gccacattgg tcaacagccg cgagccgatc accatcggcc
     7141 cgctgtcgct agaccctagc accgtggcct gggatcgcct gccggagcag ctattgggca
     7201 tggtcgagcc gcttgtcacc cagagcggcg gtgtcctcag tcgcctggcc acttcgacgg
     7261 cacgcacggt cttcaacctc tttttcatct tcgtgctgtc gatctacctg gccagtgact
     7321 tgccgcggtt tggcggctac gtgaagagct tcgcccagcg ccccggctac cgcgaggatg
     7381 ctgagcaact cctgccccgg ctgcgccacg tctggagcgc ctatctgcgc ggccagatcg
     7441 tcctatcgct ggttatcttc ctgacggtct ggattggtct gacgctgcta ggggtgcaaa
     7501 actcgctggc gctgggattg ttggcggggc tgctggagtt tctgcccaat ctaggcccgg
     7561 tcatcagcgc tgttgtaacg atactggtcg ccttcttcca gcccggtaac tacctggggc
     7621 tggcttcgtg gcagtatacg ctggtcgttc tggccctgat gatcgttatt cagcagttgg
     7681 agaaccacct tctggtgccg cgcatcgtcg gcggggcgct caatctgcac cccatcatcg
     7741 tcatcgtcgg cctgttcatc ggcgcgtcgc tcggcggcat tctgggagcg atcctggccg
     7801 ccccactgat cgcctcgatc aaggtcctga gccagtatgc ctggcgaaag ctcttcgacc
     7861 tgccgccctt ccccgtggac gagccgctcg acgacccgcc gccgcaccct ttccacccgg
     7921 aatagcccgg caatgctacc aggccggccc caccagccgg aagacctcct ccagcgtctg
     7981 ctgccccttc acctgcac
//
LOCUS       DAOSMQ010000082         7966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9059_length_7966_cov_5.508659, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000082 DAOSMQ010000000
VERSION     DAOSMQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9059_length_7966_cov_5.508659"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /gene="ruvB"
                     /locus_tag="PK829_04150"
     CDS             <1..763
                     /gene="ruvB"
                     /locus_tag="PK829_04150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOU40444.1"
                     /translation="ILSNMRTGDILFIDEVHRLSRPVEEILYPAMEDFVLDLIVGKGP
                     GAKNVRLKLPRFTVIGATTRLALMTAPLRARFGAVYRMDFYDQAAMETIVERGARILA
                     VPIAAEGTAEIARRARGTPRVALRLLRRVRDYAQVRSDGAITPATAVAALDLMEIDVL
                     GLDDLDRRVLRAIIEKFNGGPVGLETIAASISEESDTIMDVVEPYLLQLGFLERTSRG
                     RTATAHAYRHLNLPIPDTAPRTVQPGLFGNSEEPS"
     gene            769..987
                     /locus_tag="PK829_04155"
     CDS             769..987
                     /locus_tag="PK829_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOU40445.1"
                     /translation="MGRWLIVAGVVFLVVGALLLAGGRFFPWLGRLPGDIRIEGEHTR
                     VFIPITTMILLSVIGTIVLNVLVRIFRR"
     gene            984..1574
                     /locus_tag="PK829_04160"
     CDS             984..1574
                     /locus_tag="PK829_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020750464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU40446.1"
                     /translation="MSRLLLFNKPYHVLCQFTDREGRATLADYIAVPGVYAAGRLDYD
                     SEGLLLLTDDGALQSRIADPRHKLPKTYWAQVEGVPDQRAIARLRGGVRLSDGPTRPA
                     RARLLPEPDVWPRTPPIRYRANIPTAWLELTLTEGRNRQVRRMTAAAGYPTLRLIRWA
                     IGDWTLAGLAPGQWREVTAGDERRTSRQDRQERKGG"
     gene            1731..2462
                     /locus_tag="PK829_04165"
     CDS             1731..2462
                     /locus_tag="PK829_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOU40447.1"
                     /translation="MTIPTSISILGIPVHAVTMDETVALIEGYMAEARLHQIATVNPE
                     FVMAAQDDAEFRRVLTGADLCLPDGVGLLYAARRAGRRLPERVPGSELVYRLAERAAA
                     RGWPLFLLGAAPGVAEAAAAVLGDRYPGLRVAGTYAGSPDPAEDDALVARVNASGAAL
                     LYVAYGAPRQDKWIARNRDALTSVRVAIGVGGSLDFVTGRAVRAPQWAQRLGLEWLHR
                     LVKEPWRWRRMLALPRFGWRVMVGR"
     gene            complement(2479..3792)
                     /locus_tag="PK829_04170"
     CDS             complement(2479..3792)
                     /locus_tag="PK829_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40448.1"
                     /translation="MVTKHIIGWTLIALVLLTGCLSPEAQGPTGDAGLAPATLARPSA
                     TPVSVALDEPPTAAATPQVRQTPSATPTLSPTPTRTATPTVTPTATPTALPTPAPMPE
                     QLDVRICSQAPQQFNLWAQLGIRPLSALRFESEELVTFEGWAQRPESPVYSEATPKPG
                     PIPWPSSRQLLQGGQLDLSNGQLSPRPLDVGELLANPCDQTCPLEVIGQSPDGQWQLV
                     QVSDWLPEVMGLWLVSEKTTDRLVHYVPYAPDWQWAADSSLLWLVYPDPEFGSYTLVA
                     RLTDPPVIQRPEPGSVLDTYGHFLAYSPVDNAALSVPSYDWTDEPRDEVSTIPLANGL
                     ESISAIQNVPDIVTISWNDARQSFMAQVVTTNSIQFIEIPDGTTWSIPEETLKAIIPS
                     LVDVSSSLPIGLVATGEWATSASGDTLALLPNPALLWVFDCAPSG"
     gene            complement(3977..4402)
                     /locus_tag="PK829_04175"
     CDS             complement(3977..4402)
                     /locus_tag="PK829_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOU40449.1"
                     /translation="MAYLLDTCVISELVHRRPNAAVVDWLGETDERQLFLSAITIGEI
                     QRGIARLPKRTRRQELGAWLSEDLLNRFRGRLLAIDTAAMITWGTLVAELEAAGRTLP
                     AMDSLIAALARHHHLHLVTRNEKDFEGAGLTIVNPWGKQ"
     gene            complement(4405..4656)
                     /locus_tag="PK829_04180"
     CDS             complement(4405..4656)
                     /locus_tag="PK829_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015122392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOU40450.1"
                     /translation="MRTWQLQEAKNKLSQVVDEAIAAGPQVITRHGQEVAIVISSEDF
                     RSLTAERESLVDFFRRSPLSGEAIDLSRDKSPIRDVLGE"
     gene            4767..5759
                     /locus_tag="PK829_04185"
     CDS             4767..5759
                     /locus_tag="PK829_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU40451.1"
                     /translation="MRILITGSSGQIGTNLALHLLAGGHAVFGVDVRPNTWTDAFPTL
                     LQDLAAPFSDFRGGLGHAPYPPDLDAVVHFAAHAKVHELVQQPARALENITMTFNVLE
                     FCRANRLPVIFSSSREVYGDIRRYLTDESHADFAFTESPYSASKISGEALIYSYAQCY
                     GLRYLVFRFSNVYGRYDIDIERMERVIPLFIRKIARGEPITVYGENKVLDFTYVDDCV
                     AGVAQGLARLVDGRLANQTINLAYGQGNSLVTLAETIGAALGVSPRLTLAPPRVGEVT
                     HYVADIARARALLGYEPRVPLSEGIPRAVAWATEFWAAGSNGSGEKIAAQTSED"
     gene            5768..6946
                     /locus_tag="PK829_04190"
     CDS             5768..6946
                     /locus_tag="PK829_04190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU40452.1"
                     /translation="MNTNIDHDVVEMARALAAGIATRADAADRAGALPAEDVAALRDA
                     GYLGLAVPVACGGRGASLLTCVVAQVELAKGSASTALVAAMQHQVVGHQRETRSWQPD
                     AFDRIMSEAAEGGLLNHLGSEPQMGSPSRGGLPATTATPTPDGQRWLINGHKTWCTGG
                     RHLTHLLVRVRVDAAAGVDAAAGVDAAAGVVLVRQEWAGVPGLEWQETWRDAMSLRAS
                     ESHDALFHDVSVPRHYLIEVGEPKAAPNVWFPTMLSAVYLGTALAARDAVVRYALERA
                     PTALGKPIATLPRIQRQIGEIDAGLQAARALLFEVAGLWTGEGDRSAYMARIAAAKSV
                     VTATADEATEKALQVAGGSAVTRALPLERYFRDVRPAAMQPPAGDTAYEMVGRAALGM
                     "
     gene            complement(6959..7363)
                     /locus_tag="PK829_04195"
     CDS             complement(6959..7363)
                     /locus_tag="PK829_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOU40453.1"
                     /translation="MKYLLDTDMCIYLIKRRPESVIRHLAEQPVGDVDVSAVTVAELQ
                     YGVARSAQTERNAQALELFLAPLVIAGFDAGAATAYGVVRAALERSGTPIGALDALIA
                     AHALSLGAALVTHNTREFGRIPGLALANWAAG"
     gene            complement(7360..7593)
                     /gene="vapB"
                     /locus_tag="PK829_04200"
     CDS             complement(7360..7593)
                     /gene="vapB"
                     /locus_tag="PK829_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOU40454.1"
                     /translation="MDTAKLFTNGRSQAVRLPKEYRFEGTEVYIKKVGNTVVLIPTTA
                     SWQSLIHSLSEFSADFMAERAKPAAQERESPFE"
     gene            complement(7658..>7966)
                     /locus_tag="PK829_04205"
     CDS             complement(7658..>7966)
                     /locus_tag="PK829_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HOU40455.1"
                     /translation="RDIEAAVRGLGAARVTIHTVLHPPWTTEWISDAGREKLRRFGLA
                     PPPRFSSDPTGVAFFDLAECPRCGSTRTSLRNAFGPTLCRMIWYCHDCQDAFEQFKPL
                     "
BASE COUNT         1287 a         2678 c         2660 g         1341 t
ORIGIN      
        1 catcctgtcc aacatgcgca ccggtgatat cctgttcatc gacgaagtcc accgcctcag
       61 ccgcccggtg gaggagatcc tctacccggc gatggaggac tttgtgctcg acctcatcgt
      121 cggcaagggg ccgggggcca agaacgtgcg cctgaagttg ccgcgcttca ccgtcatcgg
      181 ggcgacgacg cggctggcgc tgatgaccgc gccgctgcgg gcccgctttg gcgcggtcta
      241 ccgtatggat ttctacgacc aggcggcgat ggagaccatc gtcgagcgcg gcgcgcgcat
      301 cctggccgtg cccatcgccg cggaaggcac ggccgagatc gcccggcgcg cccggggcac
      361 gccgcgcgtg gccctgcggc tgctgcggcg cgtgcgcgac tatgcccagg tgcgctccga
      421 cggggccatc acccccgcca cggcggtggc cgccctcgac ctgatggaga tcgacgtcct
      481 ggggctggac gacctcgacc ggcgcgtgct gcgggccatc atcgagaagt tcaacggcgg
      541 gccggtcggc ctggagacga tcgccgcgtc catcagcgag gaatcggaca cgatcatgga
      601 tgtcgtcgag ccgtacctgc tgcaactggg ctttctggag cgcacctcgc gcggccgcac
      661 ggccacggcc cacgcctacc gccacctgaa cctgcccatc cccgacacgg ccccgcgcac
      721 cgtccagcct ggcctgttcg gcaacagcga ggagccgtca tgagcgacct gggccgctgg
      781 ctgatcgtgg ccggagtggt gttcctggtc gtcggggcgc tgctgctggc cggcgggcgc
      841 tttttcccgt ggctcggccg cctgcccggc gatattcgca tcgagggcga gcacacgcgc
      901 gtcttcatcc ccatcacgac gatgatcctg ctgagcgtca tcggcaccat cgttctaaac
      961 gttctcgtcc gcatcttccg ccggtgagcc gcctactgct gttcaacaag ccctaccacg
     1021 tcctctgcca gttcaccgac cgcgagggcc gggccacgct ggccgactac atcgccgtgc
     1081 ccggtgtcta cgccgccggc cggctggact acgacagcga gggcctgctg ctgctcactg
     1141 acgatggcgc gttgcagagc cggattgccg acccgcgcca caagctgccc aagacctact
     1201 gggcgcaggt ggagggcgtc cccgaccagc gggccatcgc ccggctgcgc ggcggcgtgc
     1261 gcctgagcga cggcccaacg cggccggcgc gcgcccgcct gctgccggag ccggatgtct
     1321 ggccgcgcac tccgcccatc cgctatcggg ccaacatccc caccgcctgg ctggagctga
     1381 ccctgaccga ggggcgcaac cgccaggtgc ggcgcatgac cgccgccgcc ggctacccga
     1441 cgctacgcct gatccgctgg gccatcggcg actggacgct ggccggactg gccccaggcc
     1501 aatggcgaga agtaacggct ggcgacgagc gaagaacctc acgccaagat cgccaagaac
     1561 gcaaaggcgg ctaagaagaa accgcagatt acgcagatga cacagatttc ttcttgctct
     1621 tctttgcatt ctttgcgccc ttggcgagct ttgcgtgagg ttctttacct tctgccccac
     1681 tatattctgg acactggata ctgaacactg aactactgac ctactacccc atgactatcc
     1741 ctacctccat ttccatcctc ggcatccccg tccacgccgt gacgatggac gagaccgtgg
     1801 cgctgataga gggctacatg gccgaggcgc ggctgcacca gatcgccacg gtcaacccgg
     1861 agttcgtcat ggccgcgcag gacgacgccg agttccgccg ggtgctgacc ggggcggact
     1921 tgtgcctgcc ggacggcgtc ggcctgctct atgccgcccg gcgcgccggc cgccgcctgc
     1981 cggagcgcgt gccggggtcg gaactggtct accggctggc ggagcgggcg gcggcgcggg
     2041 gctggccgct gttcctgctg ggcgcggctc cgggcgtggc cgaggcggcg gcggccgttc
     2101 tgggtgaccg ctaccccggc ctgcgcgtcg ccggcacgta tgccggctcg cccgacccgg
     2161 ccgaggacga cgcccttgtc gcccgcgtca acgccagcgg cgcggcgctg ctctacgtcg
     2221 cctatggcgc gccgcggcag gacaagtgga ttgcccgcaa ccgggacgcg ctgacgagcg
     2281 tgcgcgtggc catcggcgtc ggcggctcgc tcgatttcgt caccggccgg gccgtccgcg
     2341 cgccgcagtg ggcgcaacgg ctggggctgg agtggctcca ccggctggtc aaggagccgt
     2401 ggcgctggcg gcggatgctg gcgttgccgc ggtttgggtg gcgggtgatg gtggggcgct
     2461 gacgcacaga ccgtcatgtc agccagaagg cgcgcaatcg aaaacccata gtagcgcagg
     2521 gttgggcagg agagcgagcg tgtcgcctga ggcggaggta gcccattcac ccgtagcaac
     2581 caggccaatg ggcaaggaac tggatacatc tacgagcgag gggataatcg ccttgagcgt
     2641 ttcctcggga atgctccagg tcgtgccgtc gggtatctct atgaactgga tgctgttggt
     2701 cgtcacgacc tgcgccataa agctctgcct ggcgtcgttc caactgatcg tgacaatatc
     2761 gggcacgttc tggatcgcac tgatgctttc gagaccgttg gcgagtggga tagtggatac
     2821 ctcatctctc ggctcgtcag tccagtcgta ggaaggcacg gagagggcgg cgttgtcaac
     2881 tggtgaatac gccaggaagt gaccataggt gtccagcacg ctaccaggct cgggcctctg
     2941 gatgacgggc ggatccgtca gccgggcaac caatgtatac gagccgaatt cggggtcagg
     3001 gtagacgagc cacagcaggc tgctatcggc cgcccactgc cagtctggag cgtaaggaac
     3061 atagtgaacg aggcggtctg tcgtcttctc gctgaccagc cagagtccca tgacctccgg
     3121 cagccagtca ctcacctgaa ccaactgcca ctgcccatcc ggcgactggc cgatcacttc
     3181 cagaggacaa gtctggtcac atgggttggc cagcaactcg ccaacgtcca gtgggcgagg
     3241 gctgagttgt ccgttcgaca aatccagttg tcccccttgg agcagttggc gagagcttgg
     3301 ccatggtatc ggccccggtt ttggcgtcgc ctccgagtaa acgggcgatt ccggccgctg
     3361 cgcccagcct tcaaacgtca ccagctcttc cgattcaaac cggagcgccg acaatggccg
     3421 gatgcccagt tgcgcccaca agttgaattg ttgcggcgct tgggaacaga tcctcacatc
     3481 cagttgctca ggcatcggcg ctggcgttgg cagcgctgtt ggcgttgccg ttggcgttac
     3541 tgtcggcgtc gccgttctgg tcggcgtcgg cgagagggtg ggtgtcgccg agggggtttg
     3601 ccggacttgt ggcgtcgcgg ctgccgtggg cggttcgtca agcgcaacgc tgaccggcgt
     3661 ggctgacggc cgcgccagcg tggccggggc aagaccggcg tcgcctgtcg ggccttgcgc
     3721 ttcaggcgac agacaacccg tgagcaaaac gagggcgatg agcgtccaac cgatgatgtg
     3781 cttggtgacc atgtggccct cccagtggga tcaggggcct cccacgacct ggctgggata
     3841 ttcgcataga ccgtcaggta ttgttttcta gaatgattat agttctccct gaagcggata
     3901 gctcgatgcg cccccgacga cttcgctgcg tctacccgac gatgggcatg agcagccaga
     3961 gggttgccag gtgtgcttac tgcttgcccc acggattgac aatcgtcagg ccggcgccct
     4021 cgaagtcctt ctcattgcgc gtaaccaggt gcaggtggtg atgtcgcgcc agggcagcga
     4081 tcagcgagtc cattgccgga agggtgcgcc ccgcggcctc gagttcagca acgagcgtgc
     4141 cccaggtgat catggcggct gtgtcgatgg ccagtagccg ccctctgaac cggttgagca
     4201 aatcttctga cagccatgcg cctagttcct gacggcgtgt cctcttcggc aggcgggcga
     4261 tgccccgctg aatttcgcca atggtgattg cgctcagaaa aagctggcgc tcgtccgttt
     4321 cgcctagcca atccacaacg gccgcgttcg gccgccggtg aactagctcg gagatcacgc
     4381 aagtatcgag caggtaggcc atggttattc gcccaagaca tcgcgaatag gactcttatc
     4441 gcgcgacaga tcgatcgcct cgcccgacaa aggcgaacgt cggaagaaat cgacaagtga
     4501 ttcccgctca gcagtcagag agcgaaagtc ttcggacgag atcacaatgg cgacctcttg
     4561 cccgtggcgc gtgatgacct gcggcccggc ggcgatcgcc tcatccacga cctggctaag
     4621 cttgttcttg gcttcctgta gctgccacgt ccgcattaga cccacctcca cactgctgtc
     4681 tagactgtct agattatatg tttctaccca caccctcgtc aactagcgct ttcgaaccac
     4741 aaccgctttg tgctacgata ccgcccatgc gcatcctcat caccggctcc agcggccaga
     4801 tcggcactaa cctggccctc cacctgttgg cggggggcca tgccgtcttt ggcgtcgatg
     4861 tccggccgaa cacctggact gacgccttcc cgacgctgct gcaagacctg gccgcgccct
     4921 tcagcgactt ccgcggcggc ctgggccacg cgccctaccc gcccgacctc gacgccgtcg
     4981 tccacttcgc cgcccatgcc aaggtgcatg agctggtgca gcagccggcg cgggcgctgg
     5041 aaaacattac catgaccttc aacgtgctgg agttctgccg cgccaaccgc ctgccggtca
     5101 tctttagcag ttcgcgcgag gtctatggcg acatccgccg ctacctgacc gacgaatccc
     5161 acgccgactt cgccttcacc gaaagccctt actcggccag caagatcagc ggcgaggcgc
     5221 tcatctacag ctacgcccag tgctacgggc tgcgctacct cgtctttcgc ttcagcaacg
     5281 tctacggccg ttacgacatc gacatcgagc gcatggagcg ggtcatcccc ctgttcatcc
     5341 gcaagatcgc ccgcggcgag ccgatcaccg tttacggcga gaacaaggtg ctcgatttca
     5401 cctacgtcga tgattgcgtc gccggcgtgg cgcaggggct ggcgcggctg gtcgatggcc
     5461 ggctggccaa ccagaccatc aacctggcct acgggcaggg caacagcctg gtcacgctgg
     5521 ccgagaccat cggcgcggcg ctgggcgtgt cgccgcgcct gacgctggcc ccgccgcgcg
     5581 tcggcgaggt gacccactac gtggccgaca tcgcccgggc gcgggcgctg ctgggctacg
     5641 agccgcgcgt gccgctcagc gagggcatcc cgcgcgccgt cgcctgggcc acggagtttt
     5701 gggcggccgg atcgaatggc agcggcgaga agatcgccgc gcagacatca gaggattgac
     5761 atacgccatg aataccaata tcgaccacga cgtcgtcgag atggcgcggg cgctggcggc
     5821 cggcatcgcc acgcgggccg acgccgccga ccgcgccgga gcgttgccgg ccgaggacgt
     5881 ggccgcgctg cgcgacgccg gctatctggg cctggccgtg ccggtggcct gcggcggccg
     5941 gggcgcgtcg ctgctgacgt gcgtcgtcgc gcaggtggag ttggccaaag gcagcgcttc
     6001 gaccgctctc gtcgccgcca tgcagcacca ggtcgtcggc caccagcgcg agacgcgcag
     6061 ctggcagccc gacgccttcg accgcatcat gagtgaggcg gccgagggtg gcctgctcaa
     6121 ccacctcggc agcgagccgc agatgggcag cccctcgcgc ggcggcctgc cggcaacgac
     6181 ggccacgccc acgcccgacg gccagcgctg gctcatcaac ggccacaaga cgtggtgcac
     6241 cggtggacgc cacctgacgc acttgctcgt ccgcgtgcgg gtggacgccg cagccggcgt
     6301 ggacgccgca gccggcgtgg acgccgcagc cggcgtggtg ctcgtccggc aggaatgggc
     6361 cggtgtgccg gggctggagt ggcaggagac atggcgcgat gccatgagcc tgcgcgccag
     6421 cgagagccac gacgccctct tccacgacgt cagcgtgccc cggcactacc tcatcgaggt
     6481 gggcgagccg aaggccgcgc ccaacgtctg gtttccgacc atgctgtcgg ccgtctacct
     6541 gggcacggcg ttggccgcgc gcgatgccgt cgtccgctac gccctggagc gcgcgccaac
     6601 ggccctgggc aagcccatcg ccacgctgcc gcgcatccag cgccagatcg gcgagatcga
     6661 cgccgggctg caagcggcgc gggcgctact gttcgaggtt gccgggctgt ggacgggcga
     6721 gggcgaccgc tcggcctaca tggcccgcat cgccgcggcc aagagcgtcg tcacggccac
     6781 ggccgacgag gccaccgaga aggcgctgca agtggccggc ggttcggccg tcacccgcgc
     6841 cctgccgctg gagcgctact tccgcgacgt gcgcccggca gcgatgcagc caccggcggg
     6901 ggacacggct tatgagatgg tagggcgggc ggcgctgggg atgtagtccg gagcggtctt
     6961 agccggcggc ccagttggcc agtgccaggc cggggatgcg gccgaattcg cgtgtgttat
     7021 gggtcaccag cgccgcgccg aggctgaggg cgtgggcggc gatgagcgcg tccagcgcgc
     7081 cgatgggtgt gccggagcgt tccagcgccg cgcgcaccac gccgtaggcc gtcgccgcgc
     7141 cggcgtcgaa gccggcaatg accagcggcg cgaggaacag ttccagtgcc tgggcattgc
     7201 gctctgtctg cgcgctccgg gcaacgccgt actgaagctc agctaccgtc acggccgaca
     7261 cgtcgacgtc gcccactggc tgctctgcca gatggcgaat gacgctttcc ggccggcgct
     7321 tgatcaggta gatgcacatg tcagtgtcga gcaggtactt cattcgaacg gcgactcccg
     7381 ctcctgcgcc gccggcttag cgcgctcggc catgaagtcg gcgctgaatt cgctcaggct
     7441 atggatgagc gattgccacg aggccgtcgt tgggatgagt acgacagtgt tgcccacttt
     7501 cttgatatac acctcggtgc cctcgaagcg gtactctttg ggcaggcgaa cggcctggct
     7561 gcggccgttg gtgaaaagct tggcggtgtc catcggtcac accctccata tggaatatat
     7621 cagaggtata tacctgatgc ggcggcggcg caagcgctta taagggctta aactgctcaa
     7681 aggcatcctg acaatcgtgg cagtaccaga tcatccggca cagcgtcggg ccgaaggcgt
     7741 tgcgcaggct ggtgcgggtg gagccgcagc gtggacattc ggccaggtcg aagaaggcca
     7801 cgccggtggg gtcgctgctg aagcgcggcg gcggggccag gccgaagcgg cgcaacttct
     7861 cgcggccggc gtcgctaatc cattccgtcg tccacggcgg atgcaggacc gtgtggatcg
     7921 tcacccgcgc cgcgcccagg ccgcgcacgg ccgcctcaat gtcccg
//
LOCUS       DAOSMQ010000083         7951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9083_length_7951_cov_4.374620, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000083 DAOSMQ010000000
VERSION     DAOSMQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9083_length_7951_cov_4.374620"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..931
                     /locus_tag="PK829_04210"
                     /pseudo
     CDS             1..931
                     /locus_tag="PK829_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            956..1270
                     /locus_tag="PK829_04215"
     CDS             956..1270
                     /locus_tag="PK829_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40456.1"
                     /translation="MTLDYIPLEAGLWDDVSFHKGCYTGQEIIARMESRGKLAKRLAR
                     LRPASPVAAGAAVAAAGRPVGAITSAADGPAGPLALGYVRTAALEEGWELKAGDVPVA
                     IA"
     gene            1341..1814
                     /locus_tag="PK829_04220"
     CDS             1341..1814
                     /locus_tag="PK829_04220"
                     /inference="COORDINATES: protein motif:HMM:NF025658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HOU40457.1"
                     /translation="MSLSASQPLPWDEIQQIPDPEARYIQMGRYVFAKDMDKGYGALN
                     SIERDILHVYLLESEVNNGGFDQYFINSAGDHAVEALESLWRIGAPKALALLQQAIDA
                     FEPALPSTDRAARNSQVMSLPEAMQDLWYELDQQFCTSTEPLWQLVLSYVESRQR"
     gene            1831..4032
                     /locus_tag="PK829_04225"
     CDS             1831..4032
                     /locus_tag="PK829_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOU40458.1"
                     /translation="MADFFERNLVVVYFFYGLAFFSMGLAIWLASARFRTSELRLAGP
                     LLFLAGFGGVHGLQEWHEMFHLLEMRGGSNIPGWLLLPEVRVLHLVLSFALLVYFGIC
                     LLFANRNNPHSNRYALLGAGAFVGLWALSVAITWLLDRPERLPMLDAADVLARYALAI
                     PGALIAAWAIWLEQGSFKRRGMEGFGRALLWAAVALGLYGLSQFFTPPSFLFPSTVIN
                     SDSFVALFGLPIQVVRAGLAALMALSIIRALNVFEIETQQRLAAAQTARAAAQEEALR
                     VQRQARAETEQLNRELQAAVQDLALLFTISRQTAATLDRPALLNDAVPLLVNVLPQVE
                     AGMLLLRRAADQPLEIVSIVECDDPAASVAERKAQGLQLAEYTVRAGRPSWMIDREVV
                     ALPRTDEEEAALARVAPADGDGPTALLTLASEGAGGPAVSGGPAVSGGPAVSGGHTMA
                     MPLAARGRVFGSLVFCTLYGAPPFSLRDVALAQAIADQLSVAIDNATLYEEVQQSDRQ
                     RGELLHRVVSAQEAERQRIARELHDGTGQTLTAIGLGLAAAADRLDSDPEAVRRQLAD
                     MKAMNAQVLQEVHNVIADLRPSILDNLGLLPALRGHMRAFEERTGIQTQLLVQGKSAR
                     LKPDIETTVFRIVQESLTNVVRHAAAGSVLVQIVFGERDVRLSIDDDGRGFDVAAALA
                     GSDGRAAWGLLGIAERASLVGGTAEIQSRPGEGTTVRVAIPNPFREASYGQ"
     gene            4022..4684
                     /locus_tag="PK829_04230"
     CDS             4022..4684
                     /locus_tag="PK829_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU40459.1"
                     /translation="MANEIRLLLADDHAVVRSGLRMLLQAQPDMVIIGEAETGQEAVQ
                     RAVELAPDVVLMDIEMPGMNGIEATRRIKAEAPATAVLALTMYEDDQYFFEMLRAGAS
                     GYVPKRAAPDELVSAIRAAGRGEVFLYPSLAGRLVKDYLQRGPAGEGDAQNDDLTPRE
                     QEVLTLIAEGYSNNEIADRLVISAKTVDRHRENIMRKLNLHNRVDLVKYALRKGLIGL
                     DE"
     gene            4845..5237
                     /locus_tag="PK829_04235"
     CDS             4845..5237
                     /locus_tag="PK829_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40460.1"
                     /translation="MNEFQATTVMVALFALRFVLPIALMIAIGYTMNKLADRWEADEK
                     TATAAPPIPLPVIAPLGQAAQATAAALNVPCWVFKNCDEATRARCPAYQQPSLACWVA
                     LLRAEGRLPARCAGCERYTGMPALAAGD"
     gene            5505..5828
                     /locus_tag="PK829_04240"
     CDS             5505..5828
                     /locus_tag="PK829_04240"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOU40461.1"
                     /translation="MTSTNDVYPLPESYDSDAGQPGDVEQVRWEVVERTAGITPAEII
                     VGRLQAEGIPARAWQEGAGEALGLMVGLLGTAHVVVPEEFAERARAILAADAAAYAAE
                     MEEEE"
     gene            5853..6365
                     /locus_tag="PK829_04245"
     CDS             5853..6365
                     /locus_tag="PK829_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOU40462.1"
                     /translation="MADVSWQNTDDSLVLAKPRRNRLMFIVGGVVLIAAVVYLVVNAM
                     SGSTQLYKTVGEFYAEQSRLTGRDLRVAGWVVGDSIVYTQIDAHNSRLEFDVVDDLNN
                     PAQKLHVVALNEPKPDLLQNKAQALVEGRADANGVFNANPGGLMLKCPTRYEEMAPAG
                     AGHPESIAVP"
     gene            6495..>7951
                     /gene="ccsA"
                     /locus_tag="PK829_04250"
     CDS             6495..>7951
                     /gene="ccsA"
                     /locus_tag="PK829_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOU40463.1"
                     /translation="MIADFGRITLYVAFAVALYAIVAAYYGARRGDDRWVRSARNAII
                     AVCPLVLLACGLIITALVMGDFSIAYVSNVSSRGMPTYLKVTALWGGQAGSLLFWNLL
                     LSIFTAAAMLTKWREQKELMPYVIVVLGMVQLFFLFISGVVESPFVRNLVVPADGNGL
                     NPLLRHPGMIIHPPMLYLGFVGMTIPYAFGMAALMSGQMGDNWIKTTRRWTLVAWLFL
                     SLGLILGGRWAYDVLGWGGYWGWDPVENASLMPWLAGTAFLHSVMIQEKMRMFKMWNM
                     FLIMLTFLLVISGTFVVRSGVISSVHAFAQSAIGPFFFGFMAFMFIFSLVWITKRRES
                     LRTENQLVSYLSREAAFLYNNFLIVAILAVVFLFTYYPIISELFTGEKGFVGPPVYER
                     ALAPLFAALVLLMGVAPLTMWYRTSIQRLGRAILWPAVAATALVAVLFIFGVRQWPAM
                     LGFWLVSFSVMLTLWEFAKGTRARMGKGENVWTAFT"
BASE COUNT         1262 a         2713 c         2574 g         1402 t
ORIGIN      
        1 gacgtccttc ccggacgtgg ccgcggtcct cggcccgcgc cgtcccgacg cgagcgtctg
       61 ctggtgcctg agctaccggc tgcgctcgag cgcggagaac cggtccctca ccggccccgc
      121 ccgcggggag cgggtggagc aactctgcgc cctcgacccg ccacccggcg tgctcgccta
      181 ccgggacggg gaggtggtcg gctgggcggc cgtccacccg cgggcggaca cgagcttcgc
      241 caccaaccgg cgcatcccgc acgtcgacga cctcgacgtc tggtccgtct ggtgtttccg
      301 ggtgcgtccg ggccaccgga agcagggcat cacgcacgcc ctcctcgacg gggcggtcgc
      361 gttcgcccgc gaccacggcg cgcgcgggct gctgaagttg accggcaaga cacggctcga
      421 tctgctccat cgcatgtcta cgcaggcggt caaggacctg cggccgggcg agggcgcggc
      481 gacggtgctg acgaccgaca tcggccgcat catcgaccgg gtcgtcctct atgccggcat
      541 cgatcacacc tggctgctgg ccggcgacgg ccacgccgat gccctggccc gctacctgcg
      601 gcgcaacatt ttcttcaacg acgacgtgca ggtgcatgac ctgagcgcac agatggccat
      661 cttcggcgtc tatggcccgc aggcccgtga gtatatggcc gcgctcggcc tcgatgaggg
      721 caaggagttg ccgctgcatc actgggcttc gcccggcccg cgcgccccga ccgcagccca
      781 cagcttccac cgcgccgacc cgctcggcgg cgacggctat atcatcctga cgccgtccga
      841 tacggtgggc agcctgctcg acgcgctcgc cgttgggcca tcgctgtcgg ctatcgacga
      901 ggcgacctac gactacctgc gcatcgaagc cgggcagccg cgcttcggcc gcgagttgac
      961 gctcgactac attccgctgg aggccgggct gtgggacgac gtgtcctttc acaagggctg
     1021 ctacaccggc caggagatca tcgcccgcat ggagagccgc ggcaagctgg ccaagcggct
     1081 ggcgcggttg cggccggcgt cgcccgtggc cgcgggcgcg gcggtcgccg ccgccggccg
     1141 gccggtgggc gcgatcacct cggccgccga cggcccggcc ggcccgctgg cgttgggcta
     1201 cgtcaggacg gcggcgctgg aagaaggttg ggaactaaaa gcgggtgatg tacccgtggc
     1261 aatcgcctag gctgctctta gcactgatca tcatctctca gaagggcggc gcaaagcagt
     1321 tgacgtgagg acgacaggca atgagtctat cagcatctca acctcttccc tgggacgaaa
     1381 tacagcaaat acccgaccct gaggctcgct atatccagat gggccgctac gtcttcgcta
     1441 aggatatgga caaagggtat ggcgcgctca actctataga acgcgacatc ttgcacgtgt
     1501 atctacttga aagcgaggtc aacaacggcg gtttcgatca gtacttcatc aactcggctg
     1561 gcgaccatgc cgtagaggca cttgaatcgc tctggcgcat cggcgcgccg aaggccctgg
     1621 ccctccttca acaagctatc gacgcatttg aaccggccct accctcaacc gaccgcgcag
     1681 cgcggaattc tcaagtgatg agcttgcctg aggcaatgca agacctgtgg tacgagcttg
     1741 atcagcagtt ctgcacgtcg acagagccgc tttggcagct tgtcttgagt tatgttgaaa
     1801 gccggcagcg ataggtagcg tcaactccca atggccgact tcttcgagcg caatctcgtc
     1861 gtcgtctact tcttctatgg cctggccttc ttcagcatgg gcctggccat ctggctggcc
     1921 tcggcccgct tccgcacgtc ggagttgcgt ctggccgggc cgctgctctt cctggccggc
     1981 ttcggcggcg tccacggtct gcaagagtgg cacgagatgt tccacctgct ggagatgcgc
     2041 ggcggcagca acatccccgg ctggctgctg ctgccggaag tgcgcgtcct gcacctcgtc
     2101 ctctccttcg cgttgctggt ctacttcggc atctgcctgc tcttcgccaa ccgcaacaac
     2161 ccccacagca accgctatgc cctgctgggc gcgggggcgt tcgtgggcct gtgggcgctc
     2221 agcgtggcca tcacctggct gctcgaccgg cccgagcgcc tgcccatgct cgacgccgcc
     2281 gacgtgttgg cccgctatgc cctggccatt cccggcgcgc tcatcgccgc ctgggccatc
     2341 tggctggagc agggcagctt caagcggcgg ggcatggagg gcttcggccg ggcgctgctg
     2401 tgggcggccg tggccctggg tctctacggc ctcagccagt tcttcacccc acccagcttc
     2461 ctttttccct cgacggtgat caacagcgac agcttcgtgg ccctcttcgg cttgcccatc
     2521 caggtcgttc gcgccggtct ggccgcgctc atggccctga gcatcatccg cgccctcaac
     2581 gtctttgaga tcgagacgca gcaacggctg gccgcggccc agaccgcccg cgccgccgcc
     2641 caggaagagg ccttgcgcgt ccagcgccag gcccgcgccg agacagagca gctcaaccgc
     2701 gagttgcagg ccgccgtgca agacctggcc ttgctgttca ccatctcgcg acagacggcg
     2761 gccacgctcg accggccggc gctgctcaac gatgccgtgc cgctgctggt gaacgtgcta
     2821 ccgcaggtgg aagcgggcat gctgctgttg cgccgcgccg ccgaccagcc gctggagatc
     2881 gtctccatcg tcgagtgtga tgacccggcc gcgtcggtgg ccgagcgcaa ggcccagggt
     2941 ctgcaactgg ccgagtacac cgtccgcgcc ggccggccgt cgtggatgat cgaccgcgag
     3001 gtcgtcgcct tgccgcggac ggacgaggaa gaagcggcct tggcccgcgt cgcgccggcc
     3061 gacggcgacg gcccgaccgc gttgctgacc ctggcgagtg agggagcggg cggccccgcc
     3121 gtctccggcg gccccgctgt ctccggcggc cccgccgtct ccggcggcca cacgatggcc
     3181 atgcctctgg ccgcccgcgg gcgcgtcttt ggcagcctgg tcttctgcac cctctacggc
     3241 gcgccgccgt tcagcctgcg cgacgtggcc ctggcccagg ccatcgccga ccagctcagc
     3301 gtggccatcg acaacgccac cctctatgag gaagtgcagc agagcgaccg gcagcgcggc
     3361 gagttgctcc accgtgtcgt ctcggcccag gaggccgagc ggcagcgcat cgcccgcgag
     3421 ctgcacgacg gcacgggcca gacgctgacg gccatcggcc tggggctggc cgcggccgcc
     3481 gaccggctgg acagcgaccc ggaggccgtg cgccggcagt tggccgacat gaaggccatg
     3541 aacgcgcagg tcttgcagga agtccacaac gtcatcgccg acctgcgccc gtcgatcctc
     3601 gacaatctgg gcctgctgcc cgccctgcgc ggccacatgc gcgccttcga ggagcgcacc
     3661 ggcatccaga cgcaactcct cgtccagggc aagagcgccc ggctgaagcc cgacatcgag
     3721 acgaccgtct tccgcatcgt ccaggagtcg ctgaccaacg tcgtccgcca cgccgccgcc
     3781 ggcagcgtcc tggtgcagat cgtcttcggc gagcgcgacg tgcgcctgtc aatcgacgac
     3841 gatggccgcg gcttcgacgt ggccgcggcg ctggccggca gcgacggccg ggccgcctgg
     3901 gggttgctgg gcattgccga gcgggcttca ctggtgggtg gcacagccga aatccagtcg
     3961 cgccccggcg agggcacgac cgtgcgcgtc gctattccca atcccttccg ggaggcatct
     4021 tatggccaat gaaatccgcc tgctgctagc cgacgatcac gccgttgtcc gctccggcct
     4081 gcgcatgctg ttgcaagccc aacccgacat ggtcatcatc ggcgaggctg agacgggcca
     4141 ggaggctgtc cagcgcgccg tcgagttggc ccccgacgtg gtgctgatgg acatcgagat
     4201 gccgggcatg aacggcatcg aggccacgcg ccgcattaag gccgaagcac cggccacggc
     4261 cgtgctggcc ctgaccatgt acgaagacga tcagtacttc tttgagatgt tgcgcgctgg
     4321 ggcgtcgggc tatgtgccca agcgggccgc gcccgacgag ctggtgtcgg ccatccgcgc
     4381 cgccggccgc ggcgaggtct tcctctaccc gtcgttggcc gggcggctgg tgaaggacta
     4441 cttgcagcgc ggccccgccg gcgagggcga cgcccagaac gacgatctga cgccgcgcga
     4501 gcaggaggtg ctgaccctca tcgccgaggg ctacagcaac aacgagatcg ccgaccgtct
     4561 ggtcatcagc gccaagaccg ttgaccgcca ccgcgagaac atcatgcgca agctgaacct
     4621 ccacaaccgc gtcgatctgg tcaagtacgc tctgcgcaag ggactgatcg ggctggacga
     4681 gtagcgcgcc gggcgcggac tgggtcgctt tccccatagc gaaatactac ccctacccaa
     4741 tagacacacg ggggcatctg gccgtatcct atgcttagga gccaaacctg aaaccggccg
     4801 atcgcgcgat cgtccagacc aggaacccgc tccgggagac ggcaatgaac gaattccagg
     4861 cgacgacagt gatggtagcg cttttcgcgc tgcgtttcgt gttgccgatt gcgctgatga
     4921 ttgccatcgg ctacaccatg aacaaactgg ccgaccgctg ggaagcggac gaaaagacag
     4981 ccacggccgc gccgcccatt cccttgccgg tcatcgctcc tcttggccag gcggctcagg
     5041 ccacagccgc ggcgctcaac gtcccctgct gggtttttaa gaactgcgac gaggccacgc
     5101 gcgcccgctg ccccgcctat cagcagccgt cgctggcctg ctgggtggcg ctgttgcggg
     5161 ccgaggggcg gctgccggcc aggtgcgccg gctgcgagcg ctatacgggg atgccggcgt
     5221 tggcggccgg ggactagaaa cgctctcttc caatctcctc tcacgcgggc gtagagatag
     5281 cccattgagg caaatggata gcctgccttt cccattggtg acatccatca tgctcaacgg
     5341 gtgatgatcg acgttggcaa ttggccccac gcttctataa tagacaaggt gatgacaggg
     5401 atgcccgaac ctggagttcg gcatttgcta agattggctg cttttttagt ggcgaacgcg
     5461 gtataatctt gcgtatcgga tgacgaaggt aggcggaacc aactatgaca agcacaaatg
     5521 acgtttatcc cctgccggaa agctacgaca gcgacgccgg ccaaccgggc gacgtggaac
     5581 aggtgcgctg ggaagtggtc gagcgcacgg ccggcattac cccggcagag atcatcgtcg
     5641 gccgcttgca ggccgagggc attcccgccc gcgcctggca agagggcgcc ggcgaagccc
     5701 tggggctgat ggtcgggctg ctgggcacgg cgcatgtagt cgtgccggag gagttcgccg
     5761 aacgcgcccg cgccattctg gccgccgacg ccgccgccta cgccgccgaa atggaagaag
     5821 aggagtagat tcgaccagaa ggagagatca agatggccga tgtatcctgg cagaacacgg
     5881 atgacagtct ggtgctggcc aagccgcgcc gcaaccggct gatgttcatc gtcggcggcg
     5941 tggtgctgat cgccgccgtg gtttaccttg tcgttaacgc catgagcggc agcacgcagc
     6001 tctacaagac ggtcggcgag ttctacgccg agcagagccg gctgacgggc cgcgacctgc
     6061 gcgtggccgg ctgggtcgtt ggcgactcca tcgtctatac ccagatcgac gcccacaact
     6121 cgcgtctgga gtttgacgtc gtcgatgacc tcaacaaccc ggcgcagaag cttcacgttg
     6181 tcgccctgaa cgaaccaaag cccgacctgt tgcaaaacaa ggcccaggcg ctggtcgagg
     6241 ggcgcgccga cgccaacggc gtctttaatg ccaatcccgg cgggctgatg ctgaagtgcc
     6301 cgacgcgcta cgaagagatg gcccccgcag gcgcgggcca ccccgaatcc attgccgtgc
     6361 cctaaggcgc ggggccgccg gggccggttt ggccgccggc ggccgttgat ccaacgtttg
     6421 agcgacaaat cgggcggcgg gccggggaac gcgcaagcgc tccaggcccg tcgtgctgtt
     6481 aagggaatgt ccttatgatc gctgattttg gccgcattac cctctacgtc gccttcgccg
     6541 tcgccctgta cgccatcgtt gccgcctatt atggcgcgcg ccgcggcgac gaccgctggg
     6601 tgcgcagcgc ccgcaatgcc attattgctg tctgtcccct cgtactcctg gcctgcgggc
     6661 tgatcatcac cgcgctggtg atgggcgact tctccatcgc ctacgtctcc aacgtcagca
     6721 gccgcggcat gcccacctac ctcaaggtga cggcgctctg gggcggacag gccggctcgc
     6781 tgctgttctg gaacctgctc ctgtcgatct tcaccgccgc ggccatgctg accaagtggc
     6841 gggagcaaaa agagctgatg ccctacgtca tcgtcgtgct gggcatggtg cagctcttct
     6901 tcctgttcat ctccggcgtc gtggaaagcc cgttcgtccg caacctggtc gtcccggccg
     6961 acggcaacgg cctgaacccg ctgctgcgcc accccggcat gatcatccat ccgccaatgc
     7021 tctacctggg cttcgtgggc atgaccatcc cctacgcctt cggcatggcc gcgctgatgt
     7081 cggggcagat gggcgacaac tggatcaaga ccacgcgccg ctggacgctg gtggcctggc
     7141 tgttcctcag cctggggctg atcctcggcg gccgttgggc ctacgacgtg ctcggctggg
     7201 gcggctactg gggctgggac ccggtcgaga atgcctcgct gatgccctgg ctggcgggca
     7261 cggccttcct gcactcggtc atgatccagg aaaagatgcg catgttcaag atgtggaaca
     7321 tgttcctcat catgctgacg ttcctgctgg tcatctccgg gacgtttgtc gttcgcagcg
     7381 gcgtcatctc atctgtccac gcctttgccc aatcggccat cggcccattc ttctttggct
     7441 tcatggcctt catgttcatc ttctcgctcg tctggatcac caagcggcgc gagagcctgc
     7501 gcacggagaa ccagttggtc tcctacctgt cgcgcgaggc ggcctttctt tataacaact
     7561 tcctgatcgt cgccatcctg gccgtcgtct ttctgttcac ctactacccg atcatctccg
     7621 aactatttac cggggagaag ggctttgttg ggccgcccgt ctatgagcgc gcgcttgcgc
     7681 cgctgttcgc cgcgctggtg ctgctgatgg gcgtggcccc gctgacgatg tggtatcgca
     7741 ccagcatcca acgcctcggc cgggccattc tctggccggc ggtggcggcg acggcgctgg
     7801 tcgccgtgct gttcatcttc ggcgtgcgcc agtggccggc gatgctcggc ttttggctgg
     7861 tgtccttctc ggtcatgctg accctgtggg agtttgccaa gggcacgcgg gcgcgcatgg
     7921 gcaagggcga gaacgtctgg acggccttca c
//
LOCUS       DAOSMQ010000084         7949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9085_length_7949_cov_4.973778, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000084 DAOSMQ010000000
VERSION     DAOSMQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9085_length_7949_cov_4.973778"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1057)
                     /locus_tag="PK829_04255"
     CDS             complement(11..1057)
                     /locus_tag="PK829_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOU40464.1"
                     /translation="MTDTTTSRPGRVNALGLAQVDYKGLPSTLCQGCGHNSIANQIVQ
                     VAYEMNVRPAEMLKLSGIGCSSKSPAYFLGQSFGFNTLHGRMPSLATGALMANHAMHA
                     IAVSGDGDTASIGLGQFKHLLRRNVRMVYIVENNGVYGLTKGQFSATADEGQTLKYAG
                     TNEFPPIDICMEAILAGCGFVARSFSGDPAQVRELMKAALSHRGTAVLDIISPCVAFN
                     NDDASTKSYGYGKEHEVPLHAIGFVPREAEIEIDAYDPGEMRVVEMHDGSFIQLRRLE
                     TDYDPTDRLGAMHRLQWAQTQGELITGLVYYDPARPSLAETSRLVETPLAALDDERLR
                     PGREVLAGVMSGLM"
     gene            complement(1175..>2397)
                     /locus_tag="PK829_04260"
     CDS             complement(1175..>2397)
                     /locus_tag="PK829_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40465.1"
                     /translation="ATYAVVQAEDELAAIGMVVGAGFAGARAMTATSGPGISLMAEYV
                     GLAYFAEVPAVIWDIQRVGPSTGLPTRTSQGDVLFTYYLGHGDTKNVILLPANLAECF
                     EYGYRAFDLADTLQTPVFVLSDLDLGMNNWMSPPFDYPAEPLRRGKVLSAEDVAERGF
                     ARYRDLDGDGIPYRTLPGNPHPRAAYFARGTGHNDAAVYSERPEDWESNMLRLARKFD
                     TARDLVPAPLVESVEGAPIGIIAYGTTEPALREARDRLAAAGLPTSFMRLRALPTNGD
                     VAAFIAAHDRCYVVELNRDGQMHTILSAELPGLATRLVSLAHLDGMPLTARWVVEAIT
                     AQEASRPRPAPAPAVPAPAHGSSPQVRVEGATETDLPGDGSLSPGNGLPRGDSGLTDM
                     GHDGHNRQVIGSER"
     assembly_gap    2398..2497
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2498..3075)
                     /locus_tag="PK829_04265"
     CDS             complement(<2498..3075)
                     /locus_tag="PK829_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU40466.1"
                     /translation="MSIGIAPTPAAEMAPEQPIVNDFAIVVATANGTGSQTANLAILR
                     SLFKMGIPVNGKNIFPSNIQGLPTWYHIRLSADGYTGRRHTSEVLVAFNQATVVQDIQ
                     TLPAGGICLINGDWRSVPQRDDITIYAVPVNEFIRDSGSKGKLKDYLANMVYVGALAH
                     LLDIPLARLEEALFFLFKGKQRTVDSNMAVVRA"
     gene            complement(3224..3787)
                     /locus_tag="PK829_04270"
     CDS             complement(3224..3787)
                     /locus_tag="PK829_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU40467.1"
                     /translation="MPLSPDEPIEAHISGRVPHELDDEYTDVITGQGKEADDSTSYGR
                     AGTDLDIDGLKDRLIEEHVAALGTHAPVEVEAGASLAKAIRQMTSHRIGCVLVTDETD
                     RLVGIFTERDVLMRVAGLVDDLSAVPVNAYMTPNPVAVQADLPIAHALHLMAVHGFRH
                     LPLVDEDNRPVGVISFRDVVHYLHSTS"
     gene            complement(3788..4810)
                     /locus_tag="PK829_04275"
                     /pseudo
     CDS             complement(3788..4810)
                     /locus_tag="PK829_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654465.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
     gene            complement(4892..6751)
                     /locus_tag="PK829_04280"
     CDS             complement(4892..6751)
                     /locus_tag="PK829_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOU40468.1"
                     /translation="MNDYIIEERDAVVIRFAGDSGDGMQLTGTQFTNTSAVFGNDVST
                     MPDFPAEIRAPAGTLAGVSGFQVNFSNRDILTPGDAALILVAMNPAALKASLPDLEAH
                     GTIIANVDAFTQTNLRKAGYDANPLRDASLGEYQVIEVPLTTLNREALKDIEELSTKD
                     RDRSQNFFALGLAFWMFDRSLDVTLDWVRKKFAKTPALMEANIRSLETGYHFGETSRI
                     FQHRYRIRPAALPPGTYRKVTGNTALALGLVTAAQKMGKPLFYGTYPITPASDILHAL
                     APLRNFDVRTFQAEDEIAAMGSVIGAAFGGALAVTGTSGPGIALKSEGMNLAVALELP
                     MIIVNVQRGGPSTGLPTKVEQSDLLQALFGRNGESPIAILAPYSPSDCFDIALEACRL
                     AVRSMSPVMILSDGFLANSSEPWRIPDAADIPPIPVVHPDGPTSAPHNGDGAFLPYFR
                     DAETLARPWAIPGTAGLEHRIGGLSKAPETGNVSYNPLHHERMVRERAEKIARLAEVI
                     PPQKVFGPESGELLLVSWGGTYGAVRSAVVAAQRRGRSVAHAHLRYLNPLPGNLRDVL
                     SRYRQVIVPELNGGQLAFLLRGRYALNIQSYPKVHGRPFTINEIATKIEQALS"
     gene            complement(6820..7293)
                     /locus_tag="PK829_04285"
     CDS             complement(6820..7293)
                     /locus_tag="PK829_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU40469.1"
                     /translation="MKSLKEELQSEQVRHLDLSRYCVVESGTTVRATLDRMRTDEGRP
                     VLVVRDQELVGIFTERDVLQRVMNRPETLDGPVDAVMTTSPVTVLPDTSAADALWLMD
                     DHHFRNLPVVDRQGNILGDMTYAALIAYLGARYPVEIQNRPLRPDQYPSKPEGGD"
     gene            complement(7305..>7949)
                     /locus_tag="PK829_04290"
     CDS             complement(7305..>7949)
                     /locus_tag="PK829_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOU40470.1"
                     /translation="VEHHIDPGDKLPSLNEIGGTLGVSVGKLREELAVARGLGVVSVR
                     PRLGIQREPFDFAEAILPAILFGLATGEATFAQLSKLRRTIETSFWDEAVVRLTAEDR
                     LRLRALIGRAWAKLRGEPIHIPNGEHRELHLTIFGRLDNPFVHGLLVAYWDAYDASEL
                     TRFMRYQYWIDVWTYHEQIVDALCADDFERGRRLLNEHFALLQTEPEAVVAVDI"
BASE COUNT         1271 a         2462 c         2713 g         1403 t
ORIGIN      
        1 ccacccgcgc ctacatcaac ccgctcatca ccccggccag cacctcgcgc cccggccgca
       61 gccgctcgtc gtcgagggca gccagcggcg tctccaccag ccggctcgtc tccgccagcg
      121 acggccgcgc cgggtcgtag tagaccagcc cggtgatgag ttcgccctgc gtctgcgccc
      181 actggagccg gtgcatggcc cccagccggt cggtcggatc gtagtccgtc tccagcctgc
      241 gcagctggat gaacgagccg tcgtgcatct ccacgacgcg catctcgcca gggtcgtagg
      301 cgtcgatctc gatttccgcc tcgcggggca caaagccgat ggcgtgcagc ggcacctcgt
      361 gctccttgcc gtagccatag ctcttggtgg aggcgtcgtc gttgttgaag gccacgcacg
      421 ggctgatgat gtccagcacg gccgtgccgc ggtggctgag ggcggccttc atgagttcgc
      481 gcacctgcgc cgggtcgccg gagaaggagc gggccacgaa gccgcagccg gccaggatgg
      541 cctccatgca aatgtcgatg ggcggaaact cgttcgtgcc ggcgtacttc agcgtttggc
      601 cctcgtcggc cgtggccgag aactgcccct tggtcagccc gtagacgccg ttgttctcga
      661 cgatgtagac catgcgcacg ttgcgccgca gcaggtgctt gaactggccc aggccgatgc
      721 tggctgtgtc gccgtcaccg ctgacggcga tggcgtgcat ggcgtggttg gccattagcg
      781 cgcccgtagc cagcgagggc atgcggccgt ggagggtgtt gaagccgaag ctctggccca
      841 ggaagtaggc cggcgacttg ctggagcagc caatgccgct cagcttcagc atttccgccg
      901 ggcggacgtt catctcatag gccacctgga cgatctggtt ggcaatggag ttgtggccgc
      961 agccctggca aagcgtcgag ggcaggccct tgtagtccac ctgggccagg cccagagcat
     1021 tgacgcggcc gggacgggat gttgttgtat ctgtcatcgt gttgcctctt attacccgtt
     1081 ctactgggtc ggaggaagag cctcgcgcaa agatcgctaa ggacgccaag gaattctttt
     1141 cttagcgctc ttagcgctct tggcgtgagg tttcttaccg ttcactgcca atgacctgcc
     1201 ggttgtggcc gtcatggccc atatcggtca ggccgctgtc gccacggggc aggccgttgc
     1261 cgggcgagag cgaaccgtca ccgggcaggt cggtttccgt ggccccctcg acgcgcacct
     1321 gaggcgagga cccgtgggcc ggcgcaggaa ccgccggggc cggcgcgggc cgcggccggg
     1381 acgcctcctg cgcggtgatg gcctcgacta cccaacgcgc tgtcaggggc atgccgtcca
     1441 gatgggccag cgagacgagc cgggtggcca atcccggcaa ctcggccgac aggatggtgt
     1501 gcatctgccc gtcgcggttt agctcgacga cgtagcagcg atcgtgggcg gcgatgaagg
     1561 ccgccacgtc gccgttggtg ggcagggcgc gcaggcgcat gaagctggtg ggcaggccgg
     1621 ccgcggccag ccggtcgcgc gcctcgcgca gggccggctc ggttgtgcca taggcgatga
     1681 tgccgatcgg cgcgccctcg acgctttcca ccagcggagc aggcacaagg tcgcgggcgg
     1741 tgtcgaactt gcgcgccagc cgcagcatat tgctctccca gtcttccggc cgctcgctat
     1801 agacggcggc atcgttgtgg cccgtgccac gggcgaagta ggccgcgcgc gggtgcgggt
     1861 tgccgggcag ggtgcgataa gggatgccat cgccgtcaag gtcgcgatag cgggcaaagc
     1921 cgcgttcggc cacgtcctcg gccgacagca ccttgccccg ccgtagcggc tcggccgggt
     1981 agtcgaacgg cggcgacatc cagttgttca tgcccaaatc caggtcgctg agaacgaaaa
     2041 cgggcgtttg cagcgtgtcg gccaggtcaa aggcccgata gccatactcg aagcactcgg
     2101 ccaggttggc cggcagcagg atgacgttct tggtgtcgcc gtggcccagg tagtaggtga
     2161 acaacacgtc cccctggctg gtgcgcgtcg gcaggccggt gctcggcccc acgcgctgga
     2221 tgtcccagat gacggccggc acctcggcga agtaggccag gccgacgtac tcggccatca
     2281 gcgagatgcc ggggccgctg gtggccgtca tggcccgcgc cccggcgaag cccgcgccga
     2341 cgaccatgcc gatggccgcc agctcgtcct cggcctgcac gacggcatag gtggcctnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg cgaacgacgg ccatgttgct
     2521 gtccaccgtc cgctgcttgc ccttgaacag gaagaacagc gcctcctcca gccgcgccag
     2581 ggggatgtcg agcagatggg ccagcgcgcc cacgtagacc atgttggcca ggtagtcttt
     2641 cagcttgccc ttgctgccgc tgtcacggat gaattcgttg accggcacgg cgtagatggt
     2701 gatgtcgtcg cgctggggca cgctgcgcca gtcgccgttg atcaggcaga tgccgccagc
     2761 cggcaacgtc tggatgtcct ggacgacggt ggcctggttg aaggcgacca gcacctcgga
     2821 agtgtggcgg cggccggtgt agccgtcggc gctcagacgg atgtggtacc acgtcggcaa
     2881 gccctggatg ttggacggga agatgttctt gccgttgacg gggatgccca tcttgaacag
     2941 cgaccgcaga atggccaggt tggccgtctg gctgcctgtg ccgttggccg tggccacgac
     3001 gatggcgaag tcgttgacga tcggttgctc cggggccatt tcagccgccg gcgtcggcgc
     3061 aatcccgata ctcataccat ttctccttca gactgctacc aggccggcgt gggaacccgg
     3121 cccgctgaca tcatggggcg ggtcaccaga cccgccccta ttgactgatc cactccaacc
     3181 acgcgctgtc ccggacgatt cgccggggcg ggcgacgcgg cgactaagac gtactgtgca
     3241 ggtagtgcac cacgtcgcgg aacgagatga cgcccaccgg ccggttgtct tcatccacca
     3301 atggcagatg gcggaagccg tgcacggcca tcaggtgcag cgcgtgggcg atgggcagat
     3361 cggcctggac ggcgaccggg ttgggcgtca tataggcgtt caccggcacg gccgacaggt
     3421 catcgaccag cccggccacg cgcatcagca catcccgctc ggtgaagatg cccaccagcc
     3481 ggtcggtctc gtcggtgacg aggacacagc cgatacggtg gctcgtcatc tggcggatgg
     3541 ccttggccag cgacgcgcca gcctcgacct cgacgggcgc gtgcgtgccc agagcggcaa
     3601 cgtgctcctc aatcagccgg tccttcaggc cgtcgatgtc cagatcggtg ccggcccggc
     3661 catagctggt cgagtcgtcg gcctccttgc cctggccggt gatgacgtcg gtgtactcgt
     3721 catccagctc gtggggcacg cggccggaaa tgtgggcctc aattggctcg tcgggcgaga
     3781 gcggcacaac cgtccacaga tcggcggcgc gatagagttc gtccaggttg ccggggccgc
     3841 gcttggcccg cgccgtggcg atctgctcca tcaggccgtc ggtgtaggtc ggccggtcga
     3901 ccatttgccg gaagacgccc attggcgtgg ggtactccat gttgctgagg atgtagccca
     3961 gttcgaccga gttggcgtcg tggacgacca gatcgctctc gctgacacca tcgcccaggg
     4021 tgacgacctc tggcgtgaag ccgttcaggc ggatgccacg atcgcggttc ttgccgaaga
     4081 tcatcggctg gccgtcttgc aggtagagaa tgttgtcgtc gcgcgtgcgg cggtcgtcca
     4141 gtccttccca ctcggtcggg ttgaagatga cgcagttctg gtagacctcg acgaaggacg
     4201 aacccttgtg cgcggcggcc tggcgaagca cctcgcccag gtgttgggtg tgggcgtcta
     4261 gcgagcgggc cacgaacgac gcctcggccg ccagggcgat gccaatgggg ttgatggggt
     4321 gttcgatggt gcccatcggc gacgacttgg tcttggcccc cagccgcgac gtgggcgaat
     4381 attgcccctt ggtcaggccg tagatgcggt tgttgaacag gatgatgttc aggttgacat
     4441 tgcgccgcag agcatggagc agatggttgc cgccaatcga caggccgtcg ccgtcgccgg
     4501 tgatcaccca gatgctcagt tccgggttgg tgatggccag cccggtggcc agcgtcggcg
     4561 cgcggccgtg gatgctgtgg atgccgtagg tgttcatgta gtacgggaag cggctggagc
     4621 agccgatgcc ggagatgaac acgatgttct cgcgcgggat acccagatcg ggcaacacct
     4681 tctgcacggt ggccaggatg gcataatcgc cgcagccggg acaccaacgc acggtctggt
     4741 cagaggcgaa gtcggcgcgg ctcagagccg gacgctcaac gggttgtagg ggtatggttg
     4801 cggtagtcat gttattgaat tgggaattgg gaattaggaa ttgaagaagt tgttggcgta
     4861 acgctcttca ttcctagttc ctaattcgta attagcttag cgcctgctcg atcttggtgg
     4921 cgatctcatt gatggtgaac ggccggccgt ggactttggg ataggactgg atgttgaggg
     4981 cgtagcgccc gcgcaacagg aaggccagtt gcccgccgtt cagttccggc acgatgacct
     5041 ggcgatagcg gctgagcaca tcgcgcaggt tgcccggcag cgggttcagg tagcgcagat
     5101 gggcgtgggc caccgaccgg ccgcgccgtt gggccgcgac cacggccgag cgcaccgcgc
     5161 catatgtgcc gccccagctc accaacaata gctcgcccga ctccggcccg aagacctttt
     5221 gtggcggaat gacctcggcc agccgggcga tcttctcggc ccgctcgcgc accatgcgct
     5281 cgtggtgcag cgggttatag gacacgttgc ccgtctccgg ggccttgctc agtccgccga
     5341 tgcggtgttc caggccggcc gtgccgggaa tggcccacgg ccgcgccagc gtctccgcgt
     5401 cgcggaagta gggcaggaac gcgccgtcgc cgttgtgcgg ggcgctggtc gggccgtcgg
     5461 ggtggacgac cgggatgggc gggatgtcgg ccgcgtcggg gatgcgccac ggctcgctgc
     5521 tgttggccag gaagccgtcg gacaggatca tgaccggcga catggagcgc acggccagac
     5581 ggcaggcctc cagggcgata tcaaagcagt cggacgggct atagggggcc aggatggcga
     5641 tggggctttc gccgttgcgg ccaaagaggg cctgcaacag gtcagactgc tccaccttgg
     5701 tcggcaggcc ggtgctgggg ccgccgcgct gcacgttgac gatgatcatc ggcagctcca
     5761 aagcgacagc caggttcatg ccctcgctct tgagggcgat gccggggcca ctggtgcccg
     5821 tgacggccag cgcgccgcca aaagccgcgc cgatgaccga ccccatcgcg gcgatctcgt
     5881 cctcggcctg gaaggtgcgc acgtcgaagt tacgcagggg ggccagagcg tggaggatat
     5941 cgctggccgg ggtgatggga taggtgccgt agaacagcgg cttgcccatc ttctgcgccg
     6001 ccgtcaccag ccccagcgcc agggcggtgt tgcccgtcac cttgcgatag gttcccggcg
     6061 gcagggcggc cgggcggatg cgatagcggt gctggaagat gcggctcgtc tcgccgaagt
     6121 ggtagcccgt ctccagcgag cggatgttgg cttccatcag cgccggcgtc ttggcgaact
     6181 ttttgcgcac ccagtcgagg gtcacgtcga gcgaccggtc gaacatccag aaggccagcc
     6241 ccagggcgaa gaagttctgc gagcggtccc tgtccttggt cgatagctcc tcaatgtctt
     6301 tgagcgcctc gcggttgagc gtcgtcaggg gcacttcgat gacctggtac tcgcccagcg
     6361 aagcgtcgcg cagcgggttg gcatcgtagc cggccttgcg caggttcgtc tgcgtgaagg
     6421 cgtcgacgtt ggcgatgatc gtgccgtggg cttccaggtc gggcaggctg gccttcagcg
     6481 ccgccgggtt catggccacc agaatcaggg ccgcgtcgcc gggcgtcagg atgtcacggt
     6541 tggaaaagtt cacctggaag ccgctgacgc cggccagcgt gcccgccggg gcgcgaatct
     6601 cggctggaaa gtcgggcatc gtgctgacgt cgttgccgaa gacggccgac gtgttggtaa
     6661 actgcgtgcc ggttagctgc atgccgtcgc ccgagtcacc ggcaaagcgg atcacgacgg
     6721 cgtcgcgttc ttcaatgatg tagtcgttca taggaattag gaattgcgaa ttaggaatta
     6781 ggaattacca attcgtaact cctaattcct aattcctaat tagtctccac cctccggctt
     6841 gctcggatac tgatccgggc gcagggggcg gttctgaatc tcgaccgggt agcgcgcgcc
     6901 cagataggcg atcagcgcgg cataggtcat gtcgcccagg atattgccct ggcgatcgac
     6961 cacgggcagg ttgcggaaat ggtggtcgtc catcagccag agggcatcgg cggccgacgt
     7021 gtcgggcaaa acggtgacag ggctggtggt catgaccgcg tccaccgggc cgtcgagcgt
     7081 ctccggccga ttcatcaccc gctgcaagac gtctcgctcg gtgaagatgc cgaccaactc
     7141 ttggtcgcgg acgaccagca ccggccgccc ctcatcggtg cgcatccggt cgagtgtcgc
     7201 ccgcaccgtc gtgccgctct cgacgacgca gtagcggctc aggtcgagat ggcgcacctg
     7261 ttcgctttgc agttcttcct taaggctctt catggatcac tcccctaaat atcgaccgcg
     7321 actactgctt ccggctcggt ttgcagcagc gcgaagtgtt cgttcaacag acgccggccg
     7381 cgctcgaaat catcggcgca gagggcatcg acgatctgct catgataggt ccacacgtcg
     7441 atccagtact ggtagcgcat gaagcgggtc aactcgctgg cgtcgtaggc gtcccagtag
     7501 gcgacgagga ggccgtggac aaagggattg tccagccggc cgaaaatggt caggtgtagc
     7561 tcacggtgct ccccgttggg aatgtggatc ggctcgccgc gcaacttggc ccaggctcgc
     7621 ccgatgaggg cccgcagccg cagccgatct tcagccgtca agcgtaccac agcctcgtcc
     7681 caaaagcttg tttcgatggt gcggcgcagc ttgctcagct gggcgaaggt ggcctcgccc
     7741 gtggccagcc cgaagaggat ggccggcagg atggcctcgg cgaagtcgaa aggctcgcgt
     7801 tggatgccca gccgcggccg caccgacacg acgcccaggc cccgggccac ggccagctcc
     7861 tcgcgcagct tgccaacgct aacgcccagc gtcccgccga tctcgttcag cgacggcagc
     7921 ttgtcgccgg ggtcgatgtg gtgttcgac
//
LOCUS       DAOSMQ010000085         7937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9107_length_7937_cov_4.652880, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000085 DAOSMQ010000000
VERSION     DAOSMQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9107_length_7937_cov_4.652880"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..657)
                     /locus_tag="PK829_04295"
     CDS             complement(76..657)
                     /locus_tag="PK829_04295"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU40471.1"
                     /translation="MAQLVLERAPARFALAGHSMGGRVAMEVVRMAPERVLRLALLDT
                     GHLARPAGEAGEQEADKRYALLQVARTQGVAAMAHAWVQGMVHPDRLRDAALVDAVVT
                     MFARKSADGFAAQIEALLHRPDASEVLRNIAVPTLFLCGAQDVWSSPEQHQQMCDLVP
                     GAVLDVVDNAGHMAPMERPEAVVQCFTRWLQRR"
     gene            656..1261
                     /gene="coaE"
                     /locus_tag="PK829_04300"
     CDS             656..1261
                     /gene="coaE"
                     /locus_tag="PK829_04300"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOU40472.1"
                     /translation="MAVMRLAAERGALTIDADGVVHELLASDTGIQAQVVAVFGEGVR
                     RANGGIDRAALGAIVFGDPARLGQLEAILHPAVRLAIARRVAASDAPVIVIEAIKLLD
                     EPLAAMCDQVWVTACARETQMARLRVCRGMDEASAAARIDAQSPQADKIARANVVITT
                     DGLLRETEAQVAAAWAAWESWGAQGQVGRGEEESTPGLASG"
     gene            1304..1561
                     /locus_tag="PK829_04305"
     CDS             1304..1561
                     /locus_tag="PK829_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40473.1"
                     /translation="MQAIKDWVLRHTSLAAWIVLAVGMNAILIYEARDVGLLPMQWFW
                     LILITTLVAGACIWIINWGDDEDAAAEPAAQSAPDDTKADR"
     gene            1579..2712
                     /locus_tag="PK829_04310"
     CDS             1579..2712
                     /locus_tag="PK829_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOU40474.1"
                     /translation="METPLPPEPDTRLFEQVDALRRELDGLFAVADTTLDYPNKGHIR
                     FRGRLLCEPADCYGELRRRFERYGYTPMVTTEDDGRTSVVALPVVFTHGRTRWIINLV
                     LFLLTIASTLYVGAGYGATTQAEALQIWRGWPFAASILLILGAHEMGHYFAARRHDVP
                     VTLPFFIPMPFSYFGTLGAFIQLRGPASNRRALFDVAVAGPLAGLAFALPLLLYGLAT
                     SDLGPIAPGQPFEGNSLLYLLAKLVVFGRVLPGGGVDVYLNQVAWAGWVGLFVTGLNL
                     IPVGQLDGGHIAYSLLGDRARRLFWPVILSLALIIVYSWLQGAPAVTWGLWIVLLLAF
                     GRYHARPLEDISGLDPRRRVLGVLTLALFFLCFVPLPLVAIAR"
     gene            2787..3779
                     /gene="plsX"
                     /locus_tag="PK829_04315"
     CDS             2787..3779
                     /gene="plsX"
                     /locus_tag="PK829_04315"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOU40475.1"
                     /translation="MRIVVDAMGSDDHPGPDVAGAVQAARSFGDAIILVGDQRRIEAE
                     LKAHDTAGLAIEVRHAAEAIGMKDSPSAAARDKRQSSMHVGLALVRAGEADAFVSAGN
                     TGAILAVSTLRQTGLGRISGVLRPAMGVIFPIESKPMLIDNGANADARPEYLLQFGQM
                     GSVYVERMRGIARPRVALISNGEEEGKGNKLVKEAIPLLAASGLNYVGNIEPKEFVAG
                     AAEVGVIDGFAGNIMMKTAEAVAGYMSDTIRKYIMAGTLTKIGGALARPAFRRVRDQL
                     NPDEVGGAPLLGVNGVVIIAHGRSNAYAIRQAIGQARQVAERGIVAAIAERLAR"
     gene            3870..>5128
                     /gene="fabF"
                     /locus_tag="PK829_04320"
     CDS             3870..>5128
                     /gene="fabF"
                     /locus_tag="PK829_04320"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU40476.1"
                     /translation="MKDTLLDARGRPRVVITGMGMISPLGHSVEESWANLLAGVSGIG
                     PITQFDATDLATRIAGEVKGFNARDHMDFKEARRMSRASQLTVAAARMALADAGLPER
                     APDPDRTGVHIGTAVGGIERAFEEYDVFRERGARAVNPFAMTMFLANMPSHHVSLMTG
                     STGPIGTTVAACATGTQTVGEAAELIRRGVVDTVFAGGVEGLIHEAAIAGFGAMRALS
                     THFNDAPERASRPFDRDRDGFILAEGAGIVVLERLETAVARGARIYGEVLGHATSSDA
                     YHVAAPDPEAKGAIRAMQWALDDAALSPEDVSYINAHGTSTPVNDATETLAIKRLFGE
                     RAYEVPISSTKSAMGHAMGGAGTIESIFTTLALSRGLIPPTWNYETPDPECDLDYVPN
                     VPRPADIRIALKNSFGLGGQNACLVLGR"
     assembly_gap    5129..5228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5244..5978
                     /locus_tag="PK829_04325"
     CDS             5244..5978
                     /locus_tag="PK829_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40477.1"
                     /translation="MIVLRDEKRIARLGRAAQLISLAGLLVLVGGLLIIFFSRDPRIF
                     LFQLAALVIGFALSQVGLFLSQRYLRRPRPDQVLDRAAGKFARKDGRLYHYLLPAPHV
                     LLLPTSVVVLLAKYQGGNISAHGDQWRQTGLGMRRYFGREGLGNPTREAEAMTARVAA
                     LVKDAAPNADVPVLPVIVFTTQNIDTLDVKESRLPATHFSKLSAVLRQQTMGLKPLPR
                     ADYEAIRAAFDARAAHLLEETVDADA"
     gene            5965..6723
                     /locus_tag="PK829_04330"
     CDS             5965..6723
                     /locus_tag="PK829_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU40478.1"
                     /translation="MPTPESPTPESPTPEAGLGAPSWADVLWIDAGSVNCYLCHDPDG
                     FTLIDAATPGKADVIFDRLAEFGGRPSDLKRIFITHADLDHAGSAAAVQQRSGATVYA
                     SAATAALLVEGRSPKHMPRLVQFIVDRFVRYGAVDRAAIRPVADGDELPILGRSRVLA
                     APGHTPDQYTLHCPAAGIVFAADALNTRDNRLQLSPPRMTADPSATKRTAARLLGLAP
                     RVVACGHGAPLVDDAAAVDALLAQLGGSEVGSSL"
     gene            6720..7517
                     /locus_tag="PK829_04335"
     CDS             6720..7517
                     /locus_tag="PK829_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOU40479.1"
                     /translation="MTTRALLDTELNEINQSILVMGSMVDTAIGNAVESLMTSNAAMA
                     EAVIAGDADINRLRFQIEEKCYIVLATQQPMARDLRTVIAAIHFAIELERIGDHAAGI
                     ARIVTRMSGMSLPLPEPPEFLEDEDGLADESAAEVDYVPEPVSVDGAPETAEEPPKRL
                     KLSRLPKMSKRARDMLNRAITAYMDRDAEKASAIVKRDQKINRHYRKFFAEAMQTMHT
                     QSIAELPTYLLWIAHNLERIGDRTTNLAERVVFMVTGQYTEVLEDYE"
BASE COUNT         1310 a         2657 c         2567 g         1303 t
ORIGIN      
        1 atgctcgacg tggtgctgtt cccggaaatc tggacactgc gcactgccct gtaacgcgac
       61 cagcgctccc cgacttcagc gacgctgcag ccagcgtgtg aagcattgca ccactgcttc
      121 agggcgctcc atcggcgcca tgtggcccgc gttgtccacc acatccagca ctgccccggg
      181 taccaggtcg cacatctgct ggtgctgttc tggcgacgac cagacatcct gcgccccgca
      241 caggaacagg gtgggcactg caatgttgcg caacacttcc gaggcatcgg gccgatgcaa
      301 aagcgcttca atctgggcag cgaatccatc tgcgcttttg cgtgcgaaca tggtaacgac
      361 ggcgtcgacc aaggcggcgt cgcgcaggcg atccgggtgc accatacctt gtacccaggc
      421 atgggccatg gcagccaccc cttgtgtgcg cgctacctgc agcaatgcat agcgcttgtc
      481 agcttcctgc tcgcccgcct cgcctgccgg acgcgccaga tggccggtat ccagcaaggc
      541 cagccgcagc acgcgctcgg gtgccatgcg cacgacctcc atggcaaccc tgccacccat
      601 ggagtgccct gccagcgcaa acctggccgg tgcgcgctcc agcaccagct gcgccatggc
      661 cgtgatgcgg ctggccgccg agcggggcgc gctgaccatc gacgccgatg gcgtcgtcca
      721 cgaactgctg gccagcgaca ccggcatcca ggcgcaagtg gtggccgtct ttggcgaggg
      781 cgtgcgtcgc gccaatggcg gcatcgaccg cgccgccctg ggggccatcg tcttcggcga
      841 cccggcgcgt ctgggacaac tggaggccat cctgcatccg gccgtgcggc tggccatcgc
      901 ccggcgcgtg gccgccagcg acgcgccggt gatcgtcatc gaggccatca agctgctgga
      961 cgagccgctg gccgccatgt gcgatcaggt ctgggtgacg gcctgcgcgc gcgagacgca
     1021 gatggcccgc ctgcgcgtct gccggggcat ggacgaggcc agcgccgccg cgcgcatcga
     1081 cgcgcaaagt ccccaggcag acaagatcgc ccgcgcgaat gtggtgatca ccactgatgg
     1141 gttgttgcgc gagacggagg cgcaggtggc cgcggcctgg gcggcgtggg aaagctgggg
     1201 agcacagggg caggtgggca ggggggagga agagagcacg cccggactgg cctctggtta
     1261 gaaggtgtgc atttctgggg caacctcgct atcatctaga cccatgcaag ccatcaaaga
     1321 ctgggttctg cgccatacga gtctggcggc ctggatcgtc ctggccgttg gcatgaacgc
     1381 catccttatt tacgaggcgc gcgacgtcgg cctgctgccc atgcagtggt tctggctcat
     1441 cctcatcacc acgctggtag ccggggcgtg catctggatc atcaactggg gcgacgacga
     1501 ggatgccgcc gccgagccgg ccgcccagtc tgctcccgac gacaccaagg cggatcgcta
     1561 gccagcgccg aagcccacat ggaaacgcca ctgccgccag agccggacac ccggctcttt
     1621 gaacaggtcg atgccctgcg ccgggagttg gacggcctgt tcgccgttgc cgacacgacg
     1681 ctcgactatc ccaacaaggg ccacattcgc ttccgcggac gcctgctgtg tgaaccggcc
     1741 gactgctacg gcgagttgcg ccgccgcttt gagcgttacg gctatacgcc gatggtcacc
     1801 acggaagacg acggccgcac ctcggtcgtc gctctgccgg tcgtcttcac ccacgggcgc
     1861 acgcgctgga tcatcaacct cgtcctcttc ctgctgacca tcgcctcgac gctgtacgtt
     1921 ggcgcgggct atggcgcgac gacgcaggcg gaggcgctgc aaatctggcg cggctggccc
     1981 ttcgctgcca gcatcctgct cattctgggc gcgcacgaga tgggccacta cttcgccgct
     2041 cgccgccatg atgtccccgt gacactgccc ttcttcatcc ccatgccgtt ctcctacttc
     2101 ggcacgctgg gcgcgttcat ccaactgcgc gggccggcca gtaaccggcg ggcgctattc
     2161 gacgtggccg tggccgggcc gctggcgggg ctggccttcg ctctgccact gctgctctat
     2221 ggcctggcga cctctgacct ggggccgatc gcgcccggcc agccgtttga aggcaactcg
     2281 ctcctctatc tcctggccaa gctggtcgtg ttcggccgtg tcctgcctgg cggtggcgtc
     2341 gatgtctacc tgaatcaggt cgcctgggcc ggctgggtgg ggctgttcgt caccgggttg
     2401 aatctcatac cggtggggca gcttgacgga ggccacatcg cttactcgct cctcggcgac
     2461 cgggcgcggc ggctcttctg gccggtcatt ctcagcctgg ccctcatcat tgtctactcc
     2521 tggctgcaag gcgcgccggc agtgacgtgg gggctgtgga ttgtgctgct attggccttc
     2581 ggtcgctacc acgcccggcc gctggaggac atcagcggcc tcgacccgcg ccgccgggtg
     2641 ctgggcgtgc tgaccctggc gctgttcttc ctctgctttg tccctctgcc gttggtggcc
     2701 atcgctcgct agaagaattc cgccccatcg cgaatcccca caattgtgct agaatcgccc
     2761 gttaatctgt tcacaggcag gtagctatgc ggatcgttgt tgacgcgatg gggagcgatg
     2821 accatcctgg gccggacgtg gccggggcag tgcaggccgc gcgcagtttc ggcgacgcga
     2881 tcatcctggt cggcgaccag cggcggattg aagcggaact gaaagcccac gacacggccg
     2941 ggttggccat tgaggtgcgc cacgccgccg aggccatcgg catgaaggac tcgccctcgg
     3001 ccgcggcgcg cgacaagcgg cagtcgtcga tgcacgtcgg cctggcgctg gtgcgcgctg
     3061 gcgaggccga cgctttcgtc agcgcgggca acaccggcgc aattctggct gtgtctacct
     3121 tgcgacagac cggcctgggg cgcatctctg gcgtgttgcg cccggcgatg ggtgtcatct
     3181 tccccatcga gagcaagccc atgctcatcg acaacggcgc caacgccgac gccaggccgg
     3241 aatacctgct ccagttcggc cagatgggca gcgtctacgt ggagcggatg cggggcattg
     3301 cccggccgcg cgtggccctc atctccaacg gcgaggagga gggcaagggc aacaagctgg
     3361 ttaaggaggc cattcctctg ctggcggcca gcgggctgaa ctacgtcggc aacatcgagc
     3421 cgaaagagtt cgtcgccggc gcggccgagg tcggcgttat cgacggcttt gccggcaaca
     3481 tcatgatgaa gacggccgag gccgtagccg gctacatgtc cgacaccatc cgcaagtaca
     3541 tcatggccgg cacactgacc aagatcggcg gggcgctggc gcggccggcc ttccggcgcg
     3601 tgcgcgacca gttgaaccct gatgaagtcg gcggcgcgcc acttctgggc gtcaacgggg
     3661 ttgtgattat tgctcacggc cgctccaatg cctacgccat ccggcaggcc attggccagg
     3721 cgcgacaggt ggccgagcgc ggcatcgtcg ccgccattgc cgaacggctg gcgagataac
     3781 aggtcaacgc caagcagggg ctagggacta ggggctaggg actaggaaga agctctggtc
     3841 cctggcctct tgcgataaaa ggaaacaaca tgaaagacac actgctagac gcgcgcggac
     3901 gcccgcgcgt tgtcatcacc ggcatgggca tgatcagccc gctgggccac tctgtggagg
     3961 agagctgggc taacctgctg gccggcgtct ccggcatcgg tcccatcacc caatttgacg
     4021 ccaccgacct ggccacgcgc atcgccggcg aggttaaggg cttcaacgcc cgtgaccaca
     4081 tggacttcaa ggaagcgcgg cgcatgtcgc gcgcctcgca gctgaccgtg gccgcggcgc
     4141 gcatggccct ggccgacgcc ggcttgccgg agcgcgcgcc cgaccccgac cgcaccggcg
     4201 tccacatcgg cacggccgtg ggtggcatcg agcgcgcctt cgaggagtat gacgttttcc
     4261 gcgagcgcgg cgcgcgggcg gttaacccct ttgccatgac gatgtttctg gccaacatgc
     4321 cctcccacca cgtcagcctg atgaccggat cgactgggcc aatcggcacg accgtggccg
     4381 cctgcgccac gggcacacag accgtcggcg aggcggcgga actgatccgc cgcggcgtcg
     4441 tcgatacggt gtttgccggc ggcgtggagg gtctcatcca cgaggcggcc atcgccggct
     4501 ttggggccat gcgggcgctt tcgacccact ttaacgacgc cccggagcgg gccagccggc
     4561 ccttcgaccg cgaccgcgac ggcttcatcc tggccgaggg agccggcatc gtcgtgctgg
     4621 agcggctgga gacggccgtg gcccgcggcg cgcgcatcta tggcgaagtg ctgggccacg
     4681 ccacctcgtc ggacgcctac cacgtcgccg cgcccgaccc ggaggccaag ggagccatcc
     4741 gggccatgca gtgggcgctg gacgacgccg cgctgtcgcc ggaggacgtc agctatatca
     4801 acgcccacgg cacatcgacg ccggtcaacg atgccacgga gacgctggcc atcaagcggc
     4861 tgttcggtga gcgcgcctac gaagtgccca tcagcagcac caagtcggcg atgggccacg
     4921 ccatgggcgg cgcgggcaca atcgagtcca tcttcaccac gctggccctg tcgcgcgggc
     4981 tgatcccgcc gacgtggaac tacgagacgc ccgacccgga gtgcgacttg gactacgtgc
     5041 ccaacgtgcc gcgcccggcc gacatccgca tcgccctgaa gaactcgttt gggctgggcg
     5101 ggcagaacgc ctgcctggtg ttggggcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnngc aacgaagcat actatgatcg tattgcgtga cgagaaacga atcgcccggc
     5281 tgggccgggc ggcacagctg atcagcctgg cgggcctgct ggtgttggtg ggcgggctgc
     5341 tgatcatctt tttcagccgc gacccgcgca tcttcctctt ccagttagcc gcgctggtga
     5401 ttggctttgc cttgtcgcag gtcggcctct tcctgtcgca gcgctatctg cgccggccgc
     5461 gccccgacca ggtgttggac agagccgcgg gcaagtttgc ccgcaaggac ggccgcctct
     5521 accactatct gctgcccgcg ccccacgtct tgttgctgcc aaccagcgtc gtcgtgctgc
     5581 tggccaagta ccagggcggc aacatcagcg cccacggcga ccagtggcgg cagacggggc
     5641 tgggcatgcg gcgctatttc ggccgcgaag ggttgggcaa ccccacccgc gaggccgagg
     5701 ccatgaccgc cagagtcgcc gccctggtga aggacgccgc gcccaacgcc gacgtgccgg
     5761 tgctgcccgt gatcgtcttc acgacgcaga acattgatac cctggacgtg aaggagtcgc
     5821 gcctcccggc aacgcacttc tccaagctat cggccgtgct acgccagcag acaatggggc
     5881 tgaagccatt gccccgcgcc gactacgaag ccatacgggc ggccttcgac gccagagccg
     5941 cccaccttct ggaggagacc gtcgatgccg acgcctgagt cgccgacgcc tgagtcgccg
     6001 acacctgagg ccggcctcgg cgcgccttcg tgggccgatg tgctgtggat cgacgccggc
     6061 agcgtcaact gctacctgtg ccacgatccc gacggcttca cgctcatcga cgcggccacg
     6121 cccggcaagg ccgatgtcat cttcgaccgc ctggccgagt tcggcgggcg gccctctgac
     6181 ctgaagcgta ttttcatcac ccacgccgac ctggatcatg ccggcagcgc cgcggccgtg
     6241 cagcagcgct ccggggccac ggtctacgcc tcggcggcga cggcggcgct gctggtcgag
     6301 gggcgctcgc ccaagcatat gccccggctg gtgcaattca tcgtcgatcg ctttgtgcgc
     6361 tatggggctg tggatcgcgc cgccatccgg ccggtggccg acggcgacga actgcccatc
     6421 ctgggccggt cgcgcgtgct agccgcgccg ggccacacgc ccgaccagta cacgctccat
     6481 tgtccggcgg ccggcatcgt cttcgccgcc gacgcgctaa acacgcgcga caaccggctg
     6541 caactctcgc caccgcgcat gacggctgac ccgtcggcga ccaagcgcac ggccgcgcgg
     6601 ctactgggcc ttgcgccgcg cgttgtcgcc tgtggccacg gcgcgccgtt ggtcgatgac
     6661 gccgcagctg tagacgcctt gctggcccaa ttagggggtt cggaggtagg atcgtcgcta
     6721 tgacgacacg cgccttgctc gatacggaac tcaacgagat caaccagtcc atcctggtca
     6781 tgggcagcat ggttgacacg gccatcggca atgccgtcga gtcgctgatg accagcaacg
     6841 ccgccatggc cgaagcggtc atcgccggcg acgccgacat taaccggctg cgcttccaga
     6901 tagaggagaa gtgctacatc gtcctggcca cgcagcagcc catggcccgc gacctgcgca
     6961 cggtcatcgc cgccatccac tttgccattg aactagagcg tattggcgac cacgccgccg
     7021 gcatcgctcg catcgtcacg cgcatgagcg ggatgtcgct gcccttgccg gaaccgccag
     7081 agtttctgga agatgaggac ggcctggccg acgagtcggc cgccgaagtg gactatgtgc
     7141 cggagccggt aagcgtggac ggcgcgccgg agacggccga ggagccgccc aagcgcctca
     7201 agctgtcgcg cctgcccaag atgtccaagc gcgcccgcga catgctcaac cgggccatca
     7261 cggcctacat ggatcgcgac gccgagaaag cctcggccat cgtcaagcgc gaccagaaga
     7321 tcaaccgcca ctatcgcaag ttctttgccg aagccatgca aacgatgcac acgcagagca
     7381 tcgccgagct gcccacctac ctgctgtgga tcgcccacaa cctggagcgc atcggcgacc
     7441 gcaccaccaa cctggccgag cgcgtcgtct tcatggtgac cgggcagtac accgaagtgc
     7501 tggaagatta cgagtaggcc attcggccgt agtcgtggcc gaatggctgt ttgacaagtc
     7561 gcaacgggtt cactagaatt ccatccccat agcccagaga ctgggctatg tttattgaca
     7621 tccggtagta ttgaggaggt ggtggcaggg acggaagtcg ttgtcgcaaa gagacacgag
     7681 aggtcgagag ttcgtctgtc agaagccggg cttgccccgg caacgcgccg agaaattggc
     7741 tcactgtaaa gcaaacgctt tacgatcacg ttgaaaaagg aatagaggag aggaacaatg
     7801 aaacaacgat ctttgctggc tttgctggta ttcgtattgg ccctggctct ggtggcttgt
     7861 ggcggcgggg gcacaacgga aaccccagtc gaggaagccg tctccaccga ggccccggcc
     7921 gaagccaccg aggcccc
//
LOCUS       DAOSMQ010000086         7861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9213_length_7861_cov_5.078017, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000086 DAOSMQ010000000
VERSION     DAOSMQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9213_length_7861_cov_5.078017"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PK829_04340"
     CDS             <1..1083
                     /locus_tag="PK829_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40480.1"
                     /translation="NGPRRAVLVGEPPQSVEGATTIQTDTTASALINVMPPDADEPLA
                     VLQLSGNSTARLAQATAPRFRRSDQPYVLDLEVQAGQLRFFLPPFEGRSVTMSLTTPQ
                     ATADIRDPGRYTVEVNSEATQITVQSEGAAVVTAMGETLTISQGERVEIAAGGPPTGP
                     LDPARDLIRNGDFSQGLDNWASFAWQVELPDQPKGETSVTSVGRNPVLRFTREGVGHA
                     DVRLTQSINQDVSGYDSLRLSATLRIVNQSLGVCGVQGSECPLFIIVNYIDDSGVSRV
                     WQHGFFAQGTIDDNLTPGACISCAVVQRPHERTPLGQFFFYDVDLIQELAVQGFLPPR
                     YIESVTLVGSGHTFTTEVSDIALVVE"
     gene            complement(1096..1764)
                     /gene="radC"
                     /locus_tag="PK829_04345"
     CDS             complement(1096..1764)
                     /gene="radC"
                     /locus_tag="PK829_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOU40481.1"
                     /translation="MADTDRPRERLIQIGAEALSTAELLAIVLRSGLQGENVLRLAER
                     LLIQFDHLPGLARASVTELTAANGIGPAKAAEIKAALEIGRRLVASSPEERPRVTTPD
                     DAFHLLKSEMMFQEQEHLRLILLDTRNCVLRTPTLYVGSLNTSVVRVGELFRAAIREN
                     AAALIITHNHPSGDPAPSPEDIAVTRQIVQAGKLLDVAVLDHIIIGHGRFVSLKQRGL
                     GFDR"
     gene            complement(1826..2005)
                     /locus_tag="PK829_04350"
     CDS             complement(1826..2005)
                     /locus_tag="PK829_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40482.1"
                     /translation="MNHDHDIEPDMLAETELFAVWRNEEEDGYMYHLELGGLTLHLTP
                     EEWEELVLLVRQASA"
     gene            complement(2056..3261)
                     /locus_tag="PK829_04355"
     CDS             complement(2056..3261)
                     /locus_tag="PK829_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU40483.1"
                     /translation="MTDELRPGRNNGQATRRGRRVACLGLLLALLLTACRNSGQPEAI
                     VNGAAMHRANPARTGVFQTEGLAKYSDIKWQFQAEDWVFGAPAVLGDAVYTVSYDGNV
                     YALDRETGAERWRFETEQPIIAAPAAADGLIFVAGMDGNVYALSAADGAERWRIALGS
                     GAAGDGITGSPAVVGDTVYVAGESGLLLALEAETGAERWRFQKEGQAIAFSPAVANGT
                     IYVGVANGLLYALDAATGAEQWTFDPVGEQAERYSPTADVVAGDGVVYFTTVDHNLAG
                     QLFAIDTTTHTARWVYQTDTENFSAPALAGDLLIWGGLDKSIHAISAATGEPAWDFAT
                     GDIVFSAPAVAGDLVYVGGADKNLYALNTADGQVRWQFRADSGVSSATVADGAVFVGT
                     ERGLLYALE"
     gene            complement(3279..5975)
                     /gene="acnA"
                     /locus_tag="PK829_04360"
     CDS             complement(3279..5975)
                     /gene="acnA"
                     /locus_tag="PK829_04360"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOU40484.1"
                     /translation="MTQRLFDHFGARAQLKGTPFTYYRLDKLGRIADIDRLPFSIKVL
                     LEAVLRTTDGYAVMPQDVERLARWSPQMTDSFEVPFKPGRVILQDFTGVPAVVDLAAM
                     RSAMARLGGNADRINPIVPVDLVIDHSVQVDQFGSAFALFYNAEREFERNRERYEFLK
                     WGQTAFENFRVVPPATGIVHQVNLEYLGQVVMSKPEGDDRVAFPDSLVGTDSHTTMIN
                     GLGVLGWGVGGIEAEAVMLGQPIYMLTPEVVGVRLIGGLPEGATATDLVLRITEMLRK
                     KGVVGKFVEYFGPGLSNMTLADRATIANMCPEYGATVGFFPVDDETLNYMRNTGRPAE
                     LVDLVERYTKEQGLFRTEATPDPVFSDTIELDLATIEPSLAGPKLPQDRVSLKNMKTT
                     FRQQLLAPVGPRGFGLSEADLARTAEVSNGKTTEISHGAVVIAAITSCTNTSNPSVML
                     GAGLLAKKAVEAGLHVPPYVKTSLAPGSRVVEDYLKQSGLLPYLEQLGFNIVGFGCTT
                     CIGNSGPLPEEVSQAIQEGDLVVSSVLSGNRNFEGRVHPESRSNFLASPPLVVAYALA
                     GRADIDMYNEPLGIGRDGEPVYLREIWPSNAEIKRTLAQSLTPAMFTEQYANVFSGNE
                     MWNRITRAGGAQYKWDNASTYIQEPPFFIGLSREVQPISNISGARVLVKVGDSITTDH
                     ISPAGSISRTSPAAQYLINHGVEPRDFNSYGSRRGNDRVMTRGTFANIRLRNQMAPGT
                     EGGMTTYLPTDEVMTIFDASLKYCQTNTPLVVLAGREYGTGSSRDWAAKGPLLLGVRL
                     VIAESFERIHRSNLVGMGILPLQFKPGQNPQTLGLDGTEVYATVNLSDDLKPLQDVTV
                     RAEFADGRVMTFETTCRIDTPVEIDYYRNGGILHTVLRNFLK"
     gene            6245..7552
                     /locus_tag="PK829_04365"
     CDS             6245..7552
                     /locus_tag="PK829_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOU40485.1"
                     /translation="MAVETEQDGQKHPPSASGGREEIRDPLRFFVWLTQTYGDIVQYR
                     SSVEPAYLINHPDYVQHVLQTNGQSYNKNTYLNKYMLEALTGQGLLTSENPLWREQRR
                     LIQPAFHRRSLPAFAQLMTDAAGRTVERLAAAPPGQPVDIAREMMRLTLDIVTLALFG
                     FDISGRADEIGEAMDTMVTIGKPRHRKVREAIALLDTIVFAIIDERRAHPERQRDDLL
                     TMLLNARYDDGSAMPDRQVRDEVMTLLVAGHETTANTLSWTWYLLAQSPQVVERLEAE
                     VDTLGGAAPAMADFPRLVYTDRVVQEAMRLYPSAWSISRRALADDVIGGYDIPAGAIV
                     AMSPYTIHRRPDFWPDPERFDPDRFAPELVAARPRFAYFPFGGGARQCIGNYFALMES
                     LIIIPTIAQHFRFSPCWSEPIEGHALVTLRPRGGVPVVVEKRR"
     gene            7549..>7861
                     /locus_tag="PK829_04370"
     CDS             7549..>7861
                     /locus_tag="PK829_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HOU40486.1"
                     /translation="MKVLFIGGSGIISSACTALAVDAGIDLYLLNRGRTDFRPIPAAA
                     HVLPADIREPASAAAALSGHTFDAVVDWIAFTPDHVAVDLDLFRGRVGGLVFISSASA
                     YQ"
BASE COUNT         1316 a         2564 c         2594 g         1387 t
ORIGIN      
        1 aacggccccc gccgcgctgt gcttgtcggc gagccgccgc aatccgtcga gggggcgacg
       61 accatccaga cagacacgac tgcctccgcc ctcatcaacg tcatgccgcc cgacgccgac
      121 gagccgctgg ccgtgttgca gctctccggc aactcgacgg cgcgcctggc ccaggcgacc
      181 gcgccccgct tccggcgcag cgaccagccg tatgtgctcg acctggaggt gcaggccggg
      241 cagttgcgct tctttctgcc gcccttcgag gggcgatcgg tgaccatgag cctgaccacg
      301 ccccaggcca cggccgacat acgcgatccc ggccgctaca cagtcgaggt caacagcgag
      361 gcaactcaga ttaccgtcca aagcgagggc gcggccgtcg tgacggcgat gggcgagaca
      421 ttaaccatca gccagggcga acgggtcgaa atcgccgccg gcgggccgcc aaccggcccg
      481 ctcgatccag cccgtgacct gatccgcaac ggcgacttca gccagggcct tgataactgg
      541 gcctcatttg cctggcaggt ggaactgcct gaccaaccga agggcgagac gagtgtcact
      601 tccgtcggcc gcaacccggt attgcgtttc acgcgcgaag gcgtcggcca tgccgatgtg
      661 cgcctgacgc agtcgatcaa ccaggatgtc tctggttacg actcgttgcg tctgtcggcc
      721 acgttgagaa ttgtcaatca aagcctgggc gtttgtggcg tgcagggcag cgagtgcccg
      781 ctgttcatca tcgtcaacta tatcgacgac agcggcgtga gccgcgtctg gcaacacggc
      841 ttctttgccc aggggacgat tgacgacaac ctgacgccgg gcgcgtgcat ctcctgcgct
      901 gttgtgcagc ggccgcacga acgcacgcca ctggggcagt tcttcttcta cgatgtcgat
      961 ctcattcagg aattggctgt gcagggcttc ttgccgccac gctatatcga gagcgtgacg
     1021 ctggtggggt cgggccacac cttcacgacc gaggtgtcgg acatagccct cgttgttgag
     1081 tagagcgcgc cgcgactagc ggtcgaagcc caacccccgc tgcttcagcg acacaaaccg
     1141 gccgtggccg atgatgatgt ggtcgagcac ggccacgtcg agcaacttgc ccgcctggac
     1201 gatctgccgg gtcacagcga tgtcctccgg cgagggggcc gggtcgccag aggggtggtt
     1261 gtgggtgata atgagcgcgg cggcgttttc gcggatggcg gcgcggaaca actcgccgac
     1321 gcgcacgacc gaggtgttaa ggctgccgac gtagagcgtt ggcgttcgca agacgcagtt
     1381 gcgcgtatcc agcaggatga gccgcaagtg ttcctgttcc tggaacatca tctcggactt
     1441 gagcaggtgg aacgcatcgt cgggcgtggt aacgcgcggt cgttcctctg gcgagctggc
     1501 caccagccgc cggccgatct ccagcgccgc cttgatctcg gccgccttgg ccgggccgat
     1561 gccgttggcc gcggttagct cggtcacgct ggcccgcgcc aggccgggca gatggtcaaa
     1621 ctggatgagc agccgctccg ccaggcgcaa gacgttctcg ccttgcaggc cgctgcgcag
     1681 cacgatagcc agcagctcgg ccgtcgagag ggcctcagcc ccaatctgga tgaggcgctc
     1741 tcgcggccgg tcggtgtcgg ccatgtcccg aatcatcggg acgtagcggg gccgttcgtc
     1801 accgattttc acggcgggca tcgccttagg ccgaagcctg gcgcaccagg aggactaact
     1861 cttcccactc ctccggggtc agatggagtg ttagcccgcc cagttcaagg tgatacatgt
     1921 agccgtcttc ctcttcgttg cgccagacgg cgaaaagctc ggtctcggcc agcatgtcgg
     1981 gttcaatgtc gtgatcgtga ttcatcgttg actccttatc agctttcgga taacaggatt
     2041 gcggcgcagc gacggctact ccagcgcgta gagcaggcct cgctctgtgc cgacaaagac
     2101 cgcgccgtcg gcgacggtcg cggaagagac gccggaatcg gcccggaact gccagcgcac
     2161 ctggccgtcg gccgtgttca gagcgtagag attcttgtcc gcgccgccga catagaccag
     2221 gtcgccggca acggccggcg cggagaaaac gatgtcgccc gtggcgaagt cccatgccgg
     2281 ttcgcctgtc gccgcgctga tggcgtggat gctcttatcc agcccacccc agatgagcag
     2341 gtcgccggcc agcgctggcg cggagaagtt ctcggtgtcg gtctggtaga cccagcgcgc
     2401 cgtgtgggtg gttgtatcga tggcgaagag ctggccggcg aggttgtggt cgacagtcgt
     2461 gaagtagacg acgccgtcgc cggcgaccac atcggccgtc gggctatagc gctcggcttg
     2521 ctctccgacc gggtcgaatg tccattgctc cgcgccggtc gcggcgtcca gggcatagag
     2581 aaggccattg gcgacgccca catagatcgt cccgttggcg acagccggcg aaaaggcaat
     2641 ggcctggccc tccttctgga agcgccagcg ctccgcgccc gtctccgcct ccaaagccaa
     2701 cagcaggccg ctctcgccgg caacgtagac cgtatcgccg acaacggcgg gagaaccggt
     2761 gatgccgtcg ccggcagcgc cgctgcctag agcgatgcgc cagcgctccg cgccatcagc
     2821 cgcgctcagg gcatagacgt tgccgtccat gccggcgacg aagatcaggc catcggccgc
     2881 ggccggcgcg gcgatgatgg gctgctcggt ctcgaagcgc cagcgctccg cgcccgtctc
     2941 gcgatccagc gcgtagacgt tgccgtcgta gctaacggta taaacggcat cgcccagcac
     3001 cgccggcgcg ccaaagaccc agtcctcggc ctggaactgc cacttgatgt cgctgtattt
     3061 ggccagtccc tcggtctgaa agacgccggt gcgggccgga ttggcgcggt gcatggccgc
     3121 gccgttcacg atcgcttctg gttggccgct gttgcggcag gcggtcagca gcagcgccag
     3181 gagtagcccc aggcaggcga cccgccgccc gcggcgggtc gcctgcccgt tgttgcgtcc
     3241 ggggcgcagt tcatcagtca tacgcgatcc ttggtcgttc acttcaggaa gttgcgcaag
     3301 acggtgtgga ggatgccgcc attgcgatag tagtcgatct ccaccggcgt atcgatgcgg
     3361 caggtggtct cgaaggtcat gacgcggccg tcggcgaatt cggcgcgcac cgtcacgtct
     3421 tgcaacggct tgaggtcgtc gctcaggttg accgtcgcgt agacctctgt gccgtccagc
     3481 cccagcgttt gcgggttctg gcccggctta aactggagcg gcagaatgcc catgcccacc
     3541 aggttgctgc ggtggatgcg ctcgaagctc tcggcgatga ccagacgcac gcccagcagc
     3601 agcggcccct tggcggccca gtcgcgcgag ctgcccgtgc cgtactcgcg cccggccagc
     3661 accaccagcg gcgtgttggt ctggcaatat ttcagcgagg cgtcaaagat ggtcatgacc
     3721 tcgtccgtgg gcagataggt ggtcatgccg ccctcggtgc cgggagccat ctggttgcgc
     3781 aggcggatgt tggcgaatgt gccgcgggtc atcacccggt cattgccgcg ccgcgagccg
     3841 taggagttaa agtcgcgcgg ctcgacgccg tggttgatca ggtattgcgc cgccggactg
     3901 gtgcgcgaga tagagccggc cggcgagatg tggtcggtgg tgatggagtc gccgaccttc
     3961 accaggacgc gcgcgccact gatgttgctg atgggctgta cctcgcgcga cagaccaatg
     4021 aaaaacggcg gctcctggat gtaggtcgag gcgttgtccc acttgtattg cgccccgccg
     4081 gcgcgggtga tgcggttcca catctcgttg ccgctgaaga cgttggcata ctgctcggta
     4141 aacatcgccg gcgtcagcga ctgcgccagt gtgcgtttga tctcggcgtt gctcggccag
     4201 atctcgcgca ggtagaccgg ctcgccgtcg cgaccgatgc ccagcggctc attgtacatg
     4261 tcgatgtcgg cccggccggc cagggcgtag gccactacca gcggcggcga ggccaggaag
     4321 ttgctgcgcg actccgggtg gacgcgcccc tcgaagttgc ggttgccgct gaggacggag
     4381 gagacgacca ggtcgccttc ctgaatggcc tggctgacct cttcgggcag ggggccagag
     4441 ttgccgatgc aggttgtgca gccgaaaccg acgatgttga agcccaactg ttccagatag
     4501 ggcagcaagc ccgactgctt caggtagtcc tcgacgacgc gcgagccggg ggccaggctg
     4561 gtcttgacgt agggcggcac gtgcagcccg gcttcaacgg ccttcttggc cagcaggccc
     4621 gcgccgagca tgacgctggg gttggaggtg ttggtgcagc tggtgatggc ggcgatgacg
     4681 acagcgccgt ggctgatctc cgtcgtcttg ccgttgctga cctcggccgt gcgcgccagg
     4741 tcggcctcgc tgaggccaaa gccgcgcggc cccaccgggg cgagcagctg ctggcgaaag
     4801 gtggtcttca tgttcttgag cgagacgcgg tcctgcggca gcttcggccc ggccagactc
     4861 ggctcgatag tggccaggtc cagttcgatg gtatcggaga aaacggggtc gggcgtggcc
     4921 tcggtgcgga agaggccctg ttccttcgta tagcgctcca caagatcgac aagctccgcc
     4981 gggcggccag tattgcgcat gtagttgagc gtctcgtcgt ccaccgggaa gaagcccacc
     5041 gtggccccat actccgggca catgttggcg atggtcgccc ggtcggccag ggtcatattg
     5101 ctaaggccgg ggccgaagta ctcgacaaac ttaccgacga cacccttctt gcgcagcatc
     5161 tccgtgatgc gcagcaccag gtcggttgcc gtggccccct ccggcaggcc acctatgaga
     5221 cgcacaccga ccacttccgg cgtcagcatg tagatgggct ggccgagcat gaccgcttcg
     5281 gcctcaatgc cgccgacgcc ccagcccaac acgcccaggc cgttgatcat cgtcgtgtgc
     5341 gagtcggtgc ccacgaggct atcgggaaag gccacgcggt cgtcgccctc tggcttgctc
     5401 atgaccacct ggccgaggta ttccaggttt acctggtgga cgatgcccgt ggccggaggc
     5461 acgacgcgga agttctcgaa cgccgtctgg ccccacttta gaaactcata ccgctcgcgg
     5521 ttgcgctcga attcgcgctc ggcgttatag aacagagcga aggccgagcc gaactgatcc
     5581 acctggacgg aatggtcgat gaccagatcg acggggacaa tggggttaat gcggtcggcg
     5641 ttgccgccca ggcgggccat ggccgaacgc atggcggcca ggtcgaccac agccggcacg
     5701 ccggtgaagt cttgcaggat gacgcgaccg ggcttgaagg gtacctcaaa cgagtctgtc
     5761 atctgcggcg accagcgggc cagtcgttcc acgtcctggg gcatgaccgc gtagccgtcc
     5821 gtcgttcgca gaaccgcttc cagtagtacc ttgatggaga agggcaggcg gtcgatgtcg
     5881 gcaatgcgcc ccagtttgtc gagacggtag taggtgaatg gggttccttt cagttgcgca
     5941 cgcgcgccaa aatggtcgaa gagtcgttgg gtcactgtgc acctcggtct tgtcgctgtg
     6001 cggttccgcg taaacgccgc agcgaacgtt gtataatgct gcctgttacg ggcatcacgc
     6061 tgtctatgtt atgatagaac cgagagcaat tatagcccaa ttctctggcc gggaaacttg
     6121 tcggcgagcg cgacagccgc gaacaagagg cgcaacccgg ccttcgcgcc gcgcgtagag
     6181 tgcatgggcc gtccggcccc tttgccgggc agagcgaccg gcgatcgttc acaggaggct
     6241 tgtcatggca gtagagacag aacaagatgg ccagaagcac ccgcccagcg ccagcggcgg
     6301 ccgcgaggaa atccgtgacc cgctgcgctt ctttgtctgg ttgacgcaga cctacggcga
     6361 catcgtccaa taccgctcca gcgtcgagcc ggcctatctg atcaaccacc ccgactatgt
     6421 tcagcacgtc ttgcagacca acggacagag ttacaacaag aatacctacc tcaataaata
     6481 catgctggag gcgctgaccg gacaggggct gctgaccagc gagaacccgc tctggcgcga
     6541 gcaacggcgg ctcatccagc ccgctttcca tcgccgcagc ctgcctgcct tcgcccaatt
     6601 gatgaccgac gccgccggcc gcactgtcga gcggctggcg gccgcgccgc cgggccaacc
     6661 ggtcgacatc gcccgcgaga tgatgcgcct gacgctcgac atcgttacgt tggctctttt
     6721 cggcttcgac atcagcggcc gggccgacga gatcggcgag gccatggaca cgatggtgac
     6781 catcggcaaa ccccgccacc gcaaggtgcg cgaggccata gcgttgctgg acaccatcgt
     6841 ttttgccatc atcgacgagc ggcgcgccca cccggaacga cagcgcgacg acctgctgac
     6901 catgcttctc aatgctcgct acgacgacgg cagcgccatg cccgaccggc aggtgcgcga
     6961 cgaggtgatg accctgctgg tggccggcca tgagacgacc gccaacacgc tcagttggac
     7021 gtggtacctg ctggcccagt cgccgcaggt cgtcgaacgg ctggaggccg aggtagacac
     7081 gctgggcggg gccgcgccgg cgatggccga cttcccgcgg ctggtctaca ctgaccgcgt
     7141 ggtgcaggag gccatgcgcc tctacccctc ggcctggtcg atcagccgcc gggcgctggc
     7201 cgacgatgtg atcggcggct acgacatccc cgccggggcg atcgtggcca tgagtcccta
     7261 caccatccac cggcggccgg acttctggcc cgacccggag cgcttcgatc ccgaccgctt
     7321 cgcgccggag ctggtggcgg cccggccgcg cttcgcctac ttccccttcg gcggcggcgc
     7381 gcggcagtgc atcggcaact actttgccct gatggaaagc ctgattatca tccccacgat
     7441 tgcccagcac ttccgtttca gcccgtgctg gtcggagccg atcgaggggc acgccctggt
     7501 aaccctacgg ccgcgcgggg gcgtccccgt ggtggtggag aagcgacgat gaaagttttg
     7561 ttcattggcg gcagcggcat catcagttcg gcctgcacag cgttggccgt tgacgccggc
     7621 atcgacctct atttgcttaa ccgtggccgg acggacttcc ggcccatccc ggcggcggcc
     7681 cacgtgttgc cggccgacat ccgcgagccg gcctcggccg ccgcggccct gtccggccac
     7741 accttcgacg ccgtcgtcga ttggatcgcc ttcacgcccg atcacgtcgc cgtcgatctc
     7801 gatctgttcc gcggccgggt gggcgggctc gtcttcatca gttcggcctc ggcctaccag
     7861 a
//
LOCUS       DAOSMQ010000087         7812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9293_length_7812_cov_4.321516, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000087 DAOSMQ010000000
VERSION     DAOSMQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9293_length_7812_cov_4.321516"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PK829_04375"
     CDS             <1..889
                     /locus_tag="PK829_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU40487.1"
                     /translation="ATGAPVAYSPADRWILTRLSETIATVDRLMDGYLYGEAGRQIYD
                     FLWGDFADWYVEITKVQLNAGGETTRATLDTLVTTLDYALRLLHPFIPYVTEETWQQL
                     RRAVTESGLDIVPPGGWGEALIIADWPKPIALGEGSAAADFEAVRELIRRIRNVRAEQ
                     GVAPARYIPAVIVAGDRAAFLASQRPILVALARLDDAHLSITATAEPPAQALTVALGP
                     ITAYLPLAGMVDLDAEQARLTKELAELDKQIARLTSLLDSPFAQKAPAAVVQKEREKL
                     AQLSASREEIDARLGMLAG"
     gene            1016..1336
                     /locus_tag="PK829_04380"
     CDS             1016..1336
                     /locus_tag="PK829_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004872647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOU40488.1"
                     /translation="MTTRSRPANVVADRAQRVLTITWSDGRECRYSFAGLRAVCPCVL
                     CQGGHDNMGRPADKLLLRRAQDDALNLEAIAPVGTYAVQLRWSDGHDSGIYTWEYLYE
                     ACLD"
     gene            complement(1355..2635)
                     /gene="lysA"
                     /locus_tag="PK829_04385"
     CDS             complement(1355..2635)
                     /gene="lysA"
                     /locus_tag="PK829_04385"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU40489.1"
                     /translation="MTPSLSTANSFLTDSPFRVRTGALHCEEVPLATIADAVGTPVYV
                     YSRADLLGRARTFLEATRAAAGDGTLICYAVKANANPTLLRLLREAGLGADVTSGGEL
                     FLARHVGVEPQRIIYSGVGKQAEEIEAALAAGIRAIHIESEMELDTVAAVAARLGRVA
                     PVGVRVNPNISAATHPYISTGLHSHKFGVPHERAVAILRAAAADPWLRPVGLAAHIGS
                     QITELDPFAQAARFLVALADGLADEGIRLEYLDCGGGLGIDYQGGAPGLADWVHVIGP
                     IVRAAGYELVIEPGRSIAGRAGALLTRVVYTKQQGDKRFVITDAGMTDLIRPALYQAH
                     HPILPVRARGGVEEVVDVVGPICETSDFLARDRALPPLAAGDLLAVLHAGAYGFAMSS
                     NYNGHLRPAEVLVDGDSFGVIRRRQTYDDLIAGT"
     gene            complement(2592..4577)
                     /locus_tag="PK829_04390"
     CDS             complement(2592..4577)
                     /locus_tag="PK829_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40490.1"
                     /translation="MFAGIDVAYVFGYANDVLQSIIVIFGAAVVLYNVPRLQRGSVQR
                     SFSALISLVVLVYLTELLVSRAVAPASAEFLLRLGWLGVALVPAAQFHLSDALLRTTG
                     KRSRRRRVLVVLSYLGGAAFALLGLLSNWVVGEPSALGVTPRLTGGPLFPVFTAYFWG
                     AALGGVYNVWRARHRCLTTTTRRRMTTTLATILAAPLGVFPYLMLATHPATELPLPLW
                     PVLIIGNLVVSILFAVLTSQLVYFGSASPDRVVRVRLYKYMARVPLAATIVLLVFVLV
                     SRSSGVFGLHKEVLLGFAVVATVMIVEWIIHHYKQMFERAFQLNDDPDVRRIQALSER
                     LLTTSDLKQFLESVLAATCEAVRTPSAFVAALTPLGPRLEAAIGPPATLEGLPDVALQ
                     RFSLPGSNGQGLDEPARDDSFFVWGDYWIQPLYNQRNDLMLGILGMRARAPQPDLAAE
                     ELALLARLRAQAAAALEDHILQQEVFAAVEGILPEITALQERRSAATFGALPALTAES
                     APSTDELLQDPEFGNMVRDALTHYWGGPKLTESPLLQLQVVQRELEGHDNNPVNALRA
                     ILAEAIQQQRPDGQRSLTTTEWILYNILDLKFVQGRRVRDVARRLAMSESDLYRKQRV
                     AIENVARTIANMERTILGDEEEDLDSIPVDSQFIPNG"
     gene            complement(4626..6575)
                     /gene="ftsH"
                     /locus_tag="PK829_04395"
     CDS             complement(4626..6575)
                     /gene="ftsH"
                     /locus_tag="PK829_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU40491.1"
                     /translation="MNSTRLTRWLVYLLIGAAIVAVLWSYSSAPTPQNEIPISLLAQE
                     IQANEVAEMRVSGDGREVTVVYVDSQRAPTLAAISDVSSIEEVLATYGVGSSNYADSR
                     PVVIYEQPSQWGGLLNLVGIFLPALLVIGFFYFIFRQAQGTNNQAISFGKSRARMFTG
                     DHPTIGFEDVAGCDEAKEELQEIVEFLKEPEKFVSFGARIPKGVLLVGSPGTGKTLLA
                     KAVSGEAGVPFFSIAGSEFVEMFVGVGASRVRDLFEQAKRNSPCIIFIDEIDAVGRQR
                     GAGLGGSHDEREQTLNQILVEMDGFDTDTHVIILAATNRPDILDPALLRPGRFDRRVI
                     IDRPDMRGREAIFRVHLRGKPIANAVDVAVLAKATPGFVGADIENTVNEAALLAARRN
                     RKNIGMTEFQEAIERVQLGPERKSRIMSPEEKELTAYHEAGHALVSHYLPHAQTLRKV
                     TIIPRGMAGGVTWYMDEDASFLTRSKFEAMIATALGGRVAEEIVFNEVTTGASNDLQQ
                     VTRLARAMVTQYGMSTEMGLRVYGDKQELVFLGREISEQRDYSDAIAEQIDGEVRRII
                     DSAHSLAMKILTDHRDKLELVSQRLLEVETLEASEFVALLEGKDVVVPSSTPPGSPKV
                     NLPKPSEGTERHRPALDRPPSPSPA"
     gene            complement(6619..>7812)
                     /locus_tag="PK829_04400"
     CDS             complement(6619..>7812)
                     /locus_tag="PK829_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOU40492.1"
                     /translation="LSVLASKAAPLYNHGMAADPVVLNGRYRLIDRIGSGGMSVVYRA
                     QDLSLGRIVAIKILNENLTDDEGFLRRFQREAHAAANLSHPNIVTVHDIGQDGNRHYI
                     VMEYIDGRTVKQLVRLQNQQGQPLPVGRVLDLAIQICAGIGYAHRANLIHCDVKSQNI
                     LVTRDDRVKVADFGIARALSEATQHTSDSQIWGTPHYFSPEQAAGQPASPASDVYAIG
                     VVMFELLTGRLPFIAETHTALALKHMHEPPPRASDLNPLVPPQLDQIVRKVMSKEPAA
                     RYRTADQLGRILSAYRESSLQITGPAAPVSILELAPPTYEQPTALYGRSAGQDEALGP
                     AIALHNGPWPEIDSISNAQTALYPAAGKRPERDWVAIVLGILAVVAMLGLIPLWYFVY
                     LAYRG"
BASE COUNT         1399 a         2528 c         2583 g         1302 t
ORIGIN      
        1 ggcaacgggc gcgccggtcg cctacagccc ggccgatcgc tggattctaa cccgcctgag
       61 cgaaaccatt gccaccgtcg accggctcat ggatggctat ctttacggcg aagcgggacg
      121 gcaaatctat gacttcctgt ggggcgactt cgcggactgg tacgtggaga tcaccaaagt
      181 gcaactgaat gctggcggcg aaaccacgcg cgcgacgctg gacacgctgg tgacgacact
      241 cgactacgcc ctacggttgc tgcatccgtt catcccctac gtgaccgagg agacgtggca
      301 acagttgcgc cgggcagtca cggagtccgg gctggatatt gtgccgccgg gtggctgggg
      361 cgaggcgctg atcatcgccg attggcccaa gcccatcgct ctgggcgagg gcagcgccgc
      421 ggccgacttc gaggcggtgc gcgaactcat ccgccgcatt cgcaacgtcc gcgccgaaca
      481 gggcgttgcc ccggcccgct acattcccgc cgtcatcgtc gccggcgacc gcgccgcctt
      541 tctggccagc cagcggccca tcctggtcgc cctggcccgg ctagacgacg cccacctgag
      601 tatcaccgcg accgccgaac cgccggcgca agccttaacc gtggcgctag gccccatcac
      661 cgcctacttg cctctggccg gcatggtcga tctggacgcc gagcaggccc gcctgaccaa
      721 agagctggcc gagttggaca agcagatcgc ccgattgacc agcttgctcg atagcccctt
      781 cgcccagaag gccccggcag ccgttgtgca aaaggagcgc gagaagctgg cccagctgag
      841 cgccagccgc gaagagatcg acgcacggct ggggatgctg gcgggctagg agcaagaatc
      901 actgtcagac aagcgcctgt caaacaggcg actgtcagac aagcgtggga acggccgggc
      961 gccgggagcg cccggttgct ctccacgcga cagattgtaa gaggactgaa cgaacttgac
     1021 tacgcgaagc agaccagcca acgttgtcgc cgaccgggca caacgcgtcc tgactattac
     1081 ctggagcgac ggccgcgagt gccgctactc tttcgccggc ctgcgcgccg tctgcccctg
     1141 cgtcctttgc cagggtggcc acgataacat gggccggccg gccgacaagc ttctcctccg
     1201 gcgcgcccag gacgacgccc tcaacctgga agccattgcc cccgtgggca cttacgccgt
     1261 ccaactgcgc tggagcgatg gccacgattc gggcatctac acgtgggagt atctctatga
     1321 ggcgtgtctg gactgagaca gagcggtccc ggcctcacgt tccggcgatt agatcgtcgt
     1381 aggtctgccg gcggcggatg acgccaaagc tgtccccgtc caccagcact tcggccggcc
     1441 gcaggtgacc gttgtaattg gaactcatgg cgaatccata ggccccagcg tgcagcaccg
     1501 ccagcaggtc gccggcggcc agtggcggca gcgcgcggtc gcgggccagg aagtcgctcg
     1561 tctcgcagat agggccgacg acatcgacga cttcctccac cccgccccgc gcccgaacag
     1621 gcagaatggg gtgatgggcc tggtagagtg ccggccggat gaggtcggtc atgcccgcgt
     1681 cggtgatgac gaagcgtttg tcgccctgct gcttggtata gacgactcgc gtcagcagcg
     1741 ccccggcccg gccggcgatg gagcgccccg gctcgatgac cagctcatag cccgccgccc
     1801 tgacaatcgg cccaatcaca tgcacccaat cggccaggcc cggcgcgcca ccctgatagt
     1861 cgatgcccaa cccgccgcca cagtcaaggt actccagccg gatgccctcg tccgccaggc
     1921 cgtcggccag agcaaccagg aagcgcgccg cctgagcgaa cgggtcaagc tcggtgatct
     1981 gcgagccgat gtgggcagcc aggccgacgg gccgcagcca cggatcggcc gcggcggcgc
     2041 gcaaaatggc cacggcccgc tcatggggca cgccgaactt gtggctgtgc agcccggtgc
     2101 tgatgtaggg gtgggtggcc gcgctgatgt tggggttgac acgcacgccc accggagcaa
     2161 cccgccccag ccgcgccgcg accgcggcta cggtgtccag ttccatctcc gactcgatgt
     2221 ggatggcgcg gatgccggcc gccagcgctg cctcgatctc ctcggcctgc ttgcccacgc
     2281 cggagtagat gatccgttgt ggctccaccc caacgtggcg ggccaggaat agctcgccgc
     2341 cgctggttac gtccgcgccc aggccggctt cacgcagcag gcgcagcagc gtcgggttgg
     2401 cattagcctt gacggcgtag caaatcagcg tgccgtcgcc ggccgcggcg cgcgtcgcct
     2461 ccaggaacgt ccgcgcccgg ccgagcagat cggcccggct atagacgtag acgggcgtac
     2521 ccacggcatc ggcgatggtg gccaggggaa cttcttcaca atgaagcgcg cccgtgcgga
     2581 cgcgaaaggg gctatccgtt aggaatgaat tggctgtcga cagggatgga gtcaaggtct
     2641 tcctcttcgt ctccgaggat agtccgctcc atgttggcga tggtgcgggc tacgttttca
     2701 atggccacgc gctgtttgcg atagaggtcg gattcgctca tggccaggcg gcgggctacg
     2761 tcgcggacgc ggcggccctg gacaaacttc aggtcgagga tgttgtagag gatccactcg
     2821 gtcgttgtca ggctgcgctg gccgtccggc cgctgttgct ggatagcctc ggccagaatc
     2881 gcccgcaggg cattgaccgg gttgttgtcg tgtccttcca actcgcgctg cacgacctga
     2941 agctggagca aggggctttc agtcagcttc ggcccgcccc aatagtgcgt cagcgcgtcg
     3001 cggaccatgt tgccgaactc cgggtcttgc agcaactcgt cggtcgaagg agcgctctcg
     3061 gctgtcagcg caggcaacgc gccaaacgtc gccgcgctgc gccgctcttg caacgcggtg
     3121 atctccggta agatgccctc gacggcggcg aagacttcct gctgcaagat gtggtcttcc
     3181 agggcggctg cggcctgcgc gcgcagccgc gccagcaaag ccaactcctc ggccgccaga
     3241 tcgggttggg gggcgcgcgc gcgcatgccc aggatgccga gcatcaagtc gttacgctgg
     3301 ttatagagcg gctgaatcca gtagtcgccc cagacaaaga agctgtcgtc gcgcgccggc
     3361 tcgtccagcc cctggccgtt gctgcccggc agcgagaagc gctgcagggc gacgtcgggc
     3421 agcccctcca gcgtcgccgg cgggccgatg gctgcttcca gccgcggccc caaaggcgtc
     3481 agcgcagcca caaaggccga cggcgttcgc accgcctcgc acgtggccgc cagcacgctt
     3541 tcgaggaact gcttcaggtc actggttgtc agcaggcgct cgctcagcgc ctggatgcgg
     3601 cgcacgtcgg ggtcgtcgtt gagttggaag gcgcgctcga acatctgctt gtagtggtgg
     3661 atgatccact cgacgatcat caccgtggcc accacagcaa agccaagcaa aacctctttg
     3721 tgcagcccaa agacgccgga gctgcggctg accaggacga agacgagcaa gacaatggtc
     3781 gccgccagcg gcacacgggc catgtacttg tagagccgca cgcgcacaac gcggtcgggc
     3841 gaggccgagc cgaagtagac cagttgcgac gtgagcacag cgaacaggat gctgacgacc
     3901 aggttgccaa tgattaacac cggccagagt ggcagaggca actcggtcgc ggggtgcgtg
     3961 gccaacatca ggtaggggaa gacacccagc ggcgcggcca gaatggtcgc cagggtggtg
     4021 gtcatgcgcc ggcgtgtcgt cgtcgtcagg caacgatgcc gagcgcgcca gacgttgtag
     4081 acgccaccca gagccgcgcc ccagaagtag gccgtgaaca ccgggaagag cggccccccg
     4141 gtcagccgcg gcgtcacgcc cagagcagac ggctcgccca cgacccagtt gctaagcagg
     4201 cccaacagag cgaaggccgc gccgcccaga tagctgagga cgaccagcac gcgccgtcgt
     4261 cgcgagcgct tgcctgtcgt ccgcaacagg gcatcagata gatggaattg ggccgccggc
     4321 accagggcca cgccaagcca gcccaagcgc agcaggaact cggccgacgc cggagcaaca
     4381 gcccggctaa ccagtagctc cgtcaggtaa acaaggacca ccaggctgat gagcgccgaa
     4441 aaggagcgct gcaccgaccc acgttgcagc cgggggacgt tatagaggac gaccgccgcg
     4501 ccgaaaatga caatgatgga ttgcaggaca tcattggcat aaccgaacac ataggcaacg
     4561 tcgatgccgg cgaacatacc aactgtcctg aaatgaaacg ttttcccgcc gccaggcgca
     4621 ggcgatcagg caggcgatgg cgagggtggg cgatcgagcg ctggccgatg gcgctccgtg
     4681 ccctcgctgg gcttgggcaa gttgaccttg gggctgcccg gcggcgtgct agacggcaca
     4741 acgacgtcct tgccttccaa cagggcaacg aattcggagg cttccagcgt ctcgacttcc
     4801 agcaagcgct gcgacaccaa ctccaacttg tcgcggtggt cggtcaggat tttcatcgcc
     4861 aggctgtggg cggagtcgat gatgcggcgc acctcgccgt caatctgctc ggcgatggca
     4921 tcggaatagt cgcgctgctc gctgatctcg cgccccagga agacaagctc ctgcttatcg
     4981 ccatagacgc gcagccccat ctccgtactc atgccatact gtgtcaccat cgctcgcgcc
     5041 aggcgcgtga cctgttgcag gtcgttgctg gctccggtgg tgacctcgtt aaagacgatc
     5101 tcctcggcca cgcggccgcc cagcgccgtg gcgatcatgg cctcgaactt agagcgggtc
     5161 agaaagctgg cgtcttcgtc catgtaccag gtgacgccac cggccatgcc acgcgggatg
     5221 atggtgactt tgcgcagtgt ctgcgcatgg ggcagatagt ggctgaccag agcgtggccg
     5281 gcctcgtggt aggcggtcag ctccttctcc tccggcgaca tgatgcggct cttgcgctcc
     5341 ggccccagct ggacgcgctc gatggcctcc tggaattcgg tcatgccgat gttcttcctg
     5401 ttgcgccggg ccgccagcag ggccgcctcg ttgaccgtgt tctcgatgtc cgcgccgacg
     5461 aagccaggtg tggccttggc cagcacggcc acatcgacgg cgttggcgat gggcttgccg
     5521 cgcagatgga cgcggaagat ggcctcgcgg ccgcgcatgt cgggccggtc gatgatcacc
     5581 cgccgatcga agcggccggg ccgcaacagg gccgggtcga ggatgtcagg ccggttggtg
     5641 gccgccagga tgatgacgtg cgtgtccgtg tcgaagccgt ccatttcgac aagaatctgg
     5701 ttgagtgtct gttcccgctc atcatgggag ccgcccaggc ccgcgccgcg ctgccgccca
     5761 acggcgtcaa tctcgtcgat gaagatgatg cacgggctgt tgcgcttggc ctgctcgaac
     5821 agatcgcgca cgcggctggc gccgacgccg acgaacatct ccacaaactc cgaaccagcg
     5881 atggagaaaa agggcacgcc cgcttcgccg gaaacggcct tggccaatag cgtcttgccc
     5941 gtgccggggc tgccgaccag caagacgccc ttgggaatgc gcgccccgaa gctgacgaat
     6001 ttctccggct ctttcaagaa ctcgacgatc tcttgcagct cttccttggc ctcgtcacag
     6061 ccggccacat cctcaaagcc gatggtggga tggtcgccgg tgaacatacg cgcccggctc
     6121 ttgccgaacg agatggcctg attgttggtg ccctgggcct ggcggaagat gaagtagaag
     6181 aagccgatga ccagcagagc cggcaggaaa atgccgacca ggttcagcaa cccgccccac
     6241 tggctgggct gctcgtagat cacgacaggc cggctgtcgg catagttgct gctgcccacg
     6301 ccgtaggtgg ccaacacctc ttctatcgaa ctgacatcgc tgatggccgc cagtgtgggc
     6361 gcgcgctggc tgtcgacgta gacgacggtt acctcgcggc cgtcgcccga cacccgcatc
     6421 tcggcgactt cgttcgcctg aatttcctgg gccaacaggc taatcgggat ttcattttgg
     6481 ggcgtgggcg cagagctata gctccacaat acggccacga tggccgcgcc tatcagcaga
     6541 tagactagcc acctcgtcag tcgggtcgaa ttcatgatag tagttgcctc cggctacccg
     6601 tacgcgatcg gacggatttt agcctcgata ggccaagtac acaaagtacc atagcggaat
     6661 aagccccagc atggccacga cggccaggat gccgaggaca atcgccaccc agtcgcgttc
     6721 cggccgcttg ccggcggcag ggtagagcgc tgtttgggcg ttgctgatag agtctatctc
     6781 cggccagggg ccattgtgga gggcgatcgc cgggccgagc gcttcgtcct gaccggccga
     6841 gcggccatac agcgccgtcg gctgctcata ggttggcggg gccagctcca aaatagagac
     6901 gggcgcggcc gggccggtga tctgcaagct gctctcgcgg taggcgctga ggatgcgccc
     6961 caactggtcg gctgtgcggt agcgcgccgc tggctccttc gacatgacct tgcgcacgat
     7021 ctgatccagt tgcggcggga ccaacgggtt gaggtctgac gctcgcggcg gcggctcgtg
     7081 catgtgcttc agcgccaggg ccgtgtgcgt ctcggcgatg aagggcagcc ggccggtcag
     7141 caactcgaac atcaccacac cgatggcgta gacgtcggag gcggggctgg ccggttggcc
     7201 ggcggcctgc tctggcgaga agtaatgggg cgtgccccag atctggctgt cggaggtgtg
     7261 ctgcgtcgct tcgctcaggg cgcgggcgat accgaagtcg gctaccttga cgcggtcgtc
     7321 gcgagtcacg aggatgttct gcgacttgac atcgcagtgt atcaggttgg cccggtgggc
     7381 atagccaatg ccggcgcaaa tctggatggc cagatcgagg acgcggccga caggcaacgg
     7441 ttgcccctgc tggttttgca gccgtaccag ttgtttcacc gtccgcccgt cgatgtattc
     7501 catgacgatg tagtggcggt tgccgtcctg gccgatgtcg tggacggtga cgatgttggg
     7561 gtgggaaagg ttggccgcgg cgtgggcctc gcgttggaag cggcgtagaa agccctcgtc
     7621 gtcggtcaga ttctcgttca gaatcttgat cgcgacgatg cggcccagcg aaagatcctg
     7681 cgcgcgatag acgaccgaca tgccgccact gccgatgcga tcgatgaggc ggtagcggcc
     7741 gttgaggaca accgggtctg ctgccatgcc atgattgtaa agcggagcgg ccttgctcgc
     7801 aaggacgctt ag
//
LOCUS       DAOSMQ010000088         7753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9400_length_7753_cov_5.681606, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000088 DAOSMQ010000000
VERSION     DAOSMQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9400_length_7753_cov_5.681606"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5578)
                     /locus_tag="PK829_04405"
     CDS             complement(<1..5578)
                     /locus_tag="PK829_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOU40493.1"
                     /translation="MESTDRAIAIVGVGAILPDAPSAAAFWNNIIGKRYSITETPPER
                     WSIANYYDPDPKAPDKTYSKIGGWVRGFEFDWRRFRLPPRVAAAMDEGQQWAVTIAAD
                     ALADYGYPDRPLDTERTGVILGTAMGGELHYITNQRISFPEYVGWLRRAPEFQRLPAN
                     TQTALVEQFHEVMTTTLPQITEDSMPGELPNIVSGRVANILNLRGPNFITDAACASSF
                     AAIEAAAQLLADHQVDAVVAGGVDRNMGASTYVKFCKIGALSATGTRPFGDGADGFVM
                     GEGAAAFVLKRLDDAERAGDRIYAVIRGVAGASDGKGKGITAPNPVGQQLAVQRAWEA
                     AGLDPATATLVEAHGTSTRVGDVVEVESLATMFGAAPKGSIGLGSAKSNIGHLKAGAG
                     AAGLLKTTLALHHKVLPPTLNANPSNPNIDFPQTPFYLIHEPREWTPSAGTPRRAGVS
                     AYGFGGTNFHLVIEEHVPGMMAHRQKQFAGVDIRSAGAQPGESAGPPSAAPVRPKPIR
                     GILALGANTPNELKDRLDEVTKRIEAGWTPPFELPKAVDIRAKERLVIDFGTHEELVD
                     RVQKARKVMGLDTPSAWKAMEAQGIFRGRGPKPGKLAFLFPGQGSQYVNMGRELAAVS
                     PTVKATLDEADAIMQPVLGRPLSSYLFIDSDDKAAVNQANYDLMQTEITQPAMLTLDE
                     AIRRLLADYGFAPDMVMGHSLGEYGALVAAGVLPFAESLEAAAGRGREMARVKMDDNG
                     WMAAVLAPYEVVEATLAEVDGYVVPANINSNTQSVIGGDSKAVERAIKLFQERGYQAM
                     QLPVSHAFHTRIVAPASAPLRKMLDRMHIAPPRLALVANVTGDFYPTGVTEIKDMLEK
                     QIASPVQWVKGLQTMYDAGARVFIEVGPKRALRGFVKDVFGNHEDVVALLTNHPKNGE
                     LPSFNQALCGLYAAGFGAEETVAPVAAAPAPAVATPTAPPAPVPTRETSVPATGAPVA
                     EDTNTMNQTTQPSAAASSLEALAQALTQALQGNSQAHAAPQRAYDRNETPLGSVVISG
                     TGLGLPGANKPVMDPDNALRILRGEQFVDLIPERFRNLIVQKQITRLVKGEDGSGRFE
                     TITDPAEVIKLAGRPGPFDLTEEYGVPAELVEALDITTELAMAAGIDALREAGIPLVQ
                     TYKRTTTGSYLPERWLLPESMRDDTGVIFASAFPGGDRFAEEFTRYYTWRGRKQQLEM
                     LEDLRNYTSDETTLNEIARRIDDLRDQLEREPYTFDRRFLFRILAMGHSQFAQYIGAR
                     GPNTQVNAACASTAQAVGIAEDWIRSGRCRRVVVVAADDVTGEHLIEWVGAGFLAVGA
                     ATTKDQVAEAALPFDRRRHGTIMGMGACALVVESEDAVRERGMRGIVELLSSETSNSA
                     FHGTRLDVNHIAMVMESLVAAAERRFGLNRHAIASQTVFMSHETFTPARGGSASAEVM
                     ALRHTFGESANNIIIANTKGFTGHPMGVGVEDVIAVKILEHGIVPPVPNFREVDPDLG
                     PLNLSRGGRYPVQYAIHLAAGFGSQIAFTFTRRIPGGPDRVDNKPAYRRWLADISGYD
                     TAETEVVKRTLRIVAQGNPPRTPMPNHWQPGTGPTLRTIVSGETTNVAFPARRIIPPV
                     VEKTPVAVPANGKPVAPPPVAPVAPVAPVAPQPAPAPVAEVMHAPVPPAEVKAQTAAP
                     VATQPPVVAAPATDPVADKVLAIVAAQTGYPMEMLEFDLDLEADLGIDTVKQAETFQA
                     IRAEFNIPREDSLRLRDYPTLAAVIGFVRERRPDLAAPTADDRPTTAASAAPVAAPAP
                     AAVGGQPPAGDPIADKVLAIVAAQTGYPMEMLEFDLDLEADLGIDTVKQAETFQAIRA
                     EFNIPREDSLRLRDYPT"
     gene            complement(5594..7231)
                     /locus_tag="PK829_04410"
     CDS             complement(5594..7231)
                     /locus_tag="PK829_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU40494.1"
                     /translation="MAGAGLDSETREMILDTLRKYAERKLTTDFLLELDHEDRFPEEV
                     LKELNDPMQLGLHLLFIPEAFNGLGGGAYDIYRVSELMASIDLGVATGVLATFLGSDP
                     IRVGGTEEQKAHWMGRIADEALLMAYGATEPQAGSDLAAMSTKAVPVQENGSVTGYHI
                     TGRKQWISNGAVADLYTILALAPGGPSWFVVERDAPGFSHGKPEDKHGIRASNTAALF
                     LEDVYVPTERLIGGVEGQGLAQAQAVFGYTRLMVGAFGMGAGWEALRRAIRYSHERIQ
                     GGTPLSDKQGYTHKLIVPNAARLEAARTYIEWVAERLDSGEAGLQTEGAVAKYMATES
                     GNRAAEDAIQALGGYGYTKEYMVEKIKRDVRITCIYEGTSEIMEWTIARDRWQQHLKT
                     QGTFYTDWATRLDALHAAEPRNGANYAALAMRALAVILERARLDRLTRNQHVLFRLGE
                     LIAWAETAAVFAERVSAKPTEAIALDVPARQTLARIHGRNAALKVATDGLAWAIGSGQ
                     TDPNLAASLNLPAIYAAQTGLIDDMNDARDALNSAFK"
     gene            complement(7514..7585)
                     /locus_tag="PK829_04415"
     tRNA            complement(7514..7585)
                     /locus_tag="PK829_04415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7550..7552),aa:Arg,seq:ccg)
BASE COUNT         1215 a         2418 c         2733 g         1387 t
ORIGIN      
        1 gcgtcgggta gtcgcgcagg cgcaggctgt cctcgcgcgg gatgttgaac tcggcacgga
       61 tggcctggaa ggtttcggcc tgcttgaccg tgtcgatgcc caggtcggct tccaggtcga
      121 ggtcaaactc cagcatctcc attgggtagc ccgtctgggc ggcgacgatg gccaggactt
      181 tgtcggcgat ggggtcgcca gccggcggct gaccaccgac cgcggccggc gcgggagccg
      241 caaccggcgc ggcgctggcg gcggtcgtcg gtcggtcgtc ggccgtcggc gcagccaggt
      301 cgggccgccg ctcgcggaca aagccgatga ctgcggccag cgtcgggtag tcgcgcaggc
      361 gcaggctgtc ctcgcgcggg atgttgaact cggcgcggat ggcctggaag gtttcggcct
      421 gcttgaccgt gtcgatgccc aggtcggcct ccaggtcgag gtcgaactcc agcatctcca
      481 ttgggtagcc cgtctgcgcg gcgacgatgg ccaggacttt atcggccacg gggtcggtcg
      541 caggggcagc gacaaccggc ggctgggtgg cgaccggcgc ggctgtctgc gccttcacct
      601 cggccggcgg cacgggcgcg tgcatgacct cggccacggg cgcaggcgcg ggttgcggcg
      661 cgaccggggc caccggcgcg accggggcca ccggtggcgg cgcgaccggc ttgccattgg
      721 ccggcacagc cacgggcgtc ttctcaacaa ctggcggaat gatcctgcgc gccgggaagg
      781 cgacgttggt ggtctcgccg ctgacgatgg tgcgcagcgt cgggccggtg cccggttgcc
      841 aatggttggg catgggcgtg cgcggcgggt tgccctgggc aacgatgcgc agcgtccgct
      901 tcactacctc ggtctcggcg gtatcgtagc cgctgatgtc ggccagccaa cgccggtagg
      961 cgggcttgtt gtcgacgcgg tccgggccgc cggggatgcg ccgggtgaag gtgaaggcga
     1021 tttgcgagcc aaagccggcg gccagatgga tggcgtattg caccgggtag cggccgccgc
     1081 ggctcagatt cagcggcccc aggtctgggt ccacctcgcg gaaattgggc accggcggga
     1141 caatgccatg ctccaaaatc ttgacggcga tgacatcctc aacgcccaca cccatcggat
     1201 ggccggtgaa gcccttggta ttggcgatga tgatgttgtt ggccgactcg ccgaaagtgt
     1261 ggcgcagggc catgacctcg gccgaggcgc tacccccgcg ggccggggtg aaggtctcat
     1321 gcgacatgaa gaccgtctgc gaggcaatgg cgtggcggtt gaggccaaag cggcgctcgg
     1381 cggcggcgac caggctctcc atgaccatag cgatatggtt cacgtccagg cgcgtgccgt
     1441 ggaaggcgct gttgctcgtt tcgctgctca gcagttcgac gatgccgcgc atcccgcgct
     1501 cgcggacggc gtcctcgctt tcaacgacga gggcgcacgc gcccatgccc atgatcgtgc
     1561 cgtggcggcg gcggtcgaag ggcagagcgg cctcggccac ctggtccttc gtcgtggccg
     1621 cgccaacggc caggaagccc gcgccaaccc actcgatcag gtgctcgccg gtgacgtcat
     1681 cggcggcgac aacgaccacg cgccggcacc ggccactacg aatccagtcc tcggcgatgc
     1741 cgacggcctg ggccgtgctg gcgcaggcgg cgttgacctg ggtgttgggg ccgcgcgcgc
     1801 cgatgtactg ggcgaactgg ctatggccca tagccaggat gcgaaacagg aagcggcggt
     1861 cgaaggtgta aggctcgcgc tctaactggt cgcgcaggtc gtcaatgcgc cgggcgatct
     1921 cgtttagcgt agtctcgtcg ctggtgtagt tgcgcagatc ttcgagcatc tccagctgct
     1981 gcttgcgccc gcgccaggtg tagtagcggg tgaactcctc ggcaaagcgg tcgccgccgg
     2041 ggaaggcgct ggcgaagatg acgccggtgt cgtcgcgcat gctctctggc agtagccagc
     2101 gctccggcag gtaggagccg gtggtagtgc gcttataggt ctgcaccagg ggaatgcccg
     2161 cctcgcgcaa ggcatcgata ccggcggcca tagccagctc ggtggtgatg tccagcgctt
     2221 cgaccagttc ggcgggaacg ccgtattcct cggtcagatc gaacgggcca ggccggccgg
     2281 ccagcttgat gacctcggcc ggatcggtga tggtctcgaa gcggccgctg ccatcctcgc
     2341 cctttaccag gcgggtgatc tgcttctgga cgatcaggtt acggaagcgc tccgggatga
     2401 gatcgacgaa ctgctcgccg cgcagaatgc gcagggcgtt gtcgggatcc atcaccggct
     2461 tgttcgcgcc gggcaagccc aggccggtgc cgctaatgac caccgagccg agcggcgtct
     2521 cgttgcggtc gtaggcgcgc tggggcgcgg cgtgagcctg gctgttgcct tgtaacgcct
     2581 gggtcagcgc ctgggccagg gcctcgagtg aggacgccgc cgcggaaggt tgtgttgtct
     2641 gattcatggt gttcgtatcc tcggcaaccg gcgcgccggt cgcgggaacg ctcgtctcac
     2701 gggtggggac cggggccggg ggcgcggttg gtgtcgccac ggccggcgcg ggcgcggcag
     2761 ccacgggggc gaccgtctct tccgcgccga agccggcggc atacaggccg cagagggcct
     2821 ggttgaagct gggcagctcg ccgttcttgg ggtgattggt cagtagagcg acgacatcct
     2881 cgtggttgcc aaagacgtcc ttgacaaaac cgcgcagagc gcgcttcggg ccgacctcga
     2941 tgaagacgcg cgcgccggca tcgtacatgg tctgcaagcc cttgacccac tgcaccggcg
     3001 aggcaatctg cttttccagc atgtccttga tctcggtcac gcccgtcgga tagaagtcgc
     3061 cggtgacgtt ggctaccagg gccagccggg gcggggcgat gtgcatgcgg tcgagcatct
     3121 tgcgcagagg cgcgctggcc ggagcgacga tgcgtgtgtg gaaggcgtgg ctgacgggca
     3181 actgcatggc ctggtagccg cgctcctgga aaagcttgat ggcccgctcg acggccttgc
     3241 tgtcgccgcc gatgaccgac tgcgtattgg agttgatgtt ggccggaacg acgtagccgt
     3301 cgacctcggc cagcgtggcc tcgacgacct cgtagggggc caggacggcg gccatccagc
     3361 cgttatcgtc catcttgacc cgcgccattt cgcggccgcg gccggcggcg gcctccagtg
     3421 attcggcgaa aggcagcacg cccgcggcca ccaacgcgcc atattcgccc agtgagtggc
     3481 ccatgaccat atcaggggcg aagccatagt cggccagcag acggcggatg gcctcgtcga
     3541 gcgtcagcat ggccggttgg gtgatctccg tctgcatcag atcgtaattg gcctggttga
     3601 cggccgcctt gtcgtcgctg tcgatgaaca ggtagctgct cagcggccgg ccgaggacgg
     3661 gctgcatgat ggcgtcggct tcgtcgagcg tggccttgac cgtgggcgat acggcggcca
     3721 gctcgcggcc catattgacg tattggctac cctggccggg gaagagaaag gccagcttgc
     3781 caggcttggg gccgcgaccg cggaagatgc cctgagcctc catcgccttc caggccgacg
     3841 gcgtgtctaa ccccatgacc ttgcgcgcct tctgcacccg gtcgactagc tcctcgtggg
     3901 tgccgaagtc gatgaccaac cgctccttgg cgcggatgtc cacggctttg ggcaactcga
     3961 acggcggcgt ccaacccgct tcaatgcgct tggtaacctc gtcaagacgg tccttcagct
     4021 cgttgggcgt gtttgcgccc agggccagga tgccgcggat gggtttgggg cggacgggag
     4081 cggccgatgg cgggccggca ctctcgccgg gttgcgcgcc cgcgctgcgg atgtctacgc
     4141 cggcaaattg cttctgccgg tgggccatca tccctggcac gtgctcttct atcaccagat
     4201 ggaagttcgt gccgccgaag ccgtaggcgc tgacgccagc gcggcggggc gtgccggcgc
     4261 tcggcgtcca ctcgcgcggc tcatggatca ggtagaacgg cgtctgtggg aagtcgatat
     4321 tagggttgga cgggttagcg ttgagcgttg gcgggagcac cttgtgatgc agggccagcg
     4381 tcgtcttgag cagcccggcc gcgccggcgc cggccttcag gtggccgatg ttgctcttgg
     4441 ccgagccgag gccaatgctg cctttgggag ccgcaccgaa catagtcgcc agactctcta
     4501 cctcgacgac gtcgccaacg cgcgtgctcg tgccgtgagc ctccaccagg gtggctgtgg
     4561 ccgggtccag cccggctgcc tcccaggcgc gctggacggc caactgctgg ccgaccgggt
     4621 tgggagcggt gatgccctta cccttgccgt cgctcgcgcc ggccacgccg cggatgacgg
     4681 cgtagatgcg gtcgccggcg cgctcggcgt cgtcgaggcg cttcagcacg aaggcggccg
     4741 cgccctcgcc catgacaaag ccgtcggccc cgtcgccaaa cgggcgcgta cccgtggcgc
     4801 tgagcgcgcc gatcttgcag aacttaacgt aagttgacgc gcccatgttg cggtcaacgc
     4861 caccggcgac cacggcatcg acctgatgat ccgccagaag ctgagccgcg gcctcgatgg
     4921 cggcgaagct gctggcacag gccgcgtcgg taatgaagtt tggcccacgc agattgagga
     4981 tattggccac gcggccggag acgatgttgg gcagctcgcc gggcatggaa tcttcggtga
     5041 tctgcggcag cgtcgtcgtc atgacctcgt ggaactgctc cacgagcgcc gtctgcgtgt
     5101 tggccggcag gcgctggaac tctggcgcgc ggcgcagcca gccaacgtac tccgggaagg
     5161 agatacgctg gttggtaatg tagtgcagtt cgccgcccat ggctgtgccc aggatgacgc
     5221 cagtgcgctc ggtgtcaagc ggccggtcag ggtagccgta gtcggccaaa gcgtcggcgg
     5281 cgatggtgac ggcccactgc tggccctcgt ccatggccgc ggcgacgcgc ggcggcaggc
     5341 ggaagcgccg ccaatcgaat tcaaagcccc gcacccagcc gccgattttg ctgtaagtct
     5401 tgtctggcgc tttggggtca ggatcatagt agttggcaat gctccagcgc tccggtggtg
     5461 tttcggtgat gctgtagcgc ttgccgatga tgttgttcca gaacgcggcg gcgctcggcg
     5521 cgtcgggcaa gatggccccc acgccgacga tggcaatggc gcgatcggtt gattccatga
     5581 tggcattccc tcgttatttg aacgcgctgt tcagggcatc acgcgcgtca ttcatgtcgt
     5641 cgataagccc ggtctgcgcg gcgtagatgg ccggcagatt gagcgaggcg gcgaggttcg
     5701 ggtcggtctg gccggagcca atggcccacg cgaggccgtc ggtggccacc ttcagggcag
     5761 cgttgcggcc gtggatgcgg gccagtgtct ggcgggcggg cacatccagg gcgatggctt
     5821 ctgtcggctt ggcgctgacg cgctcggcga agacggccgc cgtctcagcc caggcgatca
     5881 gctcgcccag gcggaagagg acgtgctggt tgcgcgtcag ccggtcgagg cgggcgcgct
     5941 ccaggatgac agccagggcg cgcatggcca gggcagcgta gttggctccg ttgcgtggct
     6001 cggcggcgtg caacgcgtct agccgggtgg cccagtcggt gtaaaaagtg ccctgcgtct
     6061 tgagatgttg ctgccagcga tcgcgagcaa tggtccactc cataatctcc gacgtgcctt
     6121 cgtagatgca ggtaatgcgc acgtcgcgct tgatcttctc gaccatatac tctttggtgt
     6181 agccatagcc gcccaacgcc tggatagcgt cttcggcggc acggttgccc gactcggtgg
     6241 ccatgtactt ggcgacggcc ccttcggtct gcaagccggc ttcgccgcta tcgaggcgct
     6301 cagcaaccca ctcgatgtag gtgcgggccg cttccagccg cgcggcattg gggacgatga
     6361 gcttatgggt gtagccttgc ttgtcgctga ggggcgtgcc gccctggatg cgctcatggg
     6421 agtagcggat ggcgcggcgc agcgcttccc agcccgcgcc catgccgaac gcgccgacca
     6481 tcagccgggt gtagccgaag acggcctgcg cctgggctag gccctgccct tcgacgccac
     6541 cgatgagccg ctccgtcggg acgtagacat cctccaggaa gagggccgcg gtgttgctgg
     6601 cgcggatgcc atgcttgtct tccggtttgc cgtggctgaa gccgggcgcg tcgcgttcga
     6661 cgacgaacca cgacgggccg ccgggcgcga gagcaagaat ggtgtacaga tcggccacgg
     6721 cgccattgct gatccactgc ttgcggccgg taatgtggta gccggtgacg ctgccattct
     6781 cctgcacggg gacggccttg gtgctcatcg ccgccaggtc gctgcccgcc tgcggctccg
     6841 tcgcgccata ggccatcagc aacgcctcat cagcgatacg gcccatccaa tgagccttct
     6901 gctcttctgt gccgcctacg cgaatggggt cggagccgag gaaggtggcc aggacgcccg
     6961 tagctacgcc gaggtcgatg ctggccatca gttcggagac gcggtagata tcatacgcgc
     7021 cgccgcccag accgttaaat gcctcgggaa tgaacaagag atgcagacca agctgcattg
     7081 ggtcattgag ttctttcaga acctcttccg gaaaccgatc ctcgtgatcc aactccagca
     7141 ggaagtcggt tgtcagcttg cgctcggcat acttgcgcaa ggtatcgagg atcatctcgc
     7201 gagtctcgct gtctaatcct gcaccagcca tgttattctc cttcagtaac ctgtgggcca
     7261 cgcgacggca gaccgggcag tacttgggaa ggcaccatca ttcgcccagg gcgagcgatt
     7321 aggcctgcgt ctctcgtgcc tggtctacat tctacggcaa ggggtttatg acttttagtg
     7381 acgaatgtaa tgtaaaacag ggtcaaaagg catgatgcaa cgtgtcatga gagctggcac
     7441 ccagagaagc aacagtgcat agaccgggaa ctctcatctg tgcgccgggg tcatcgcgta
     7501 aagggtagct gagtgccctc gacaggactc gaacctgtgc acctggcttc ggaggccagt
     7561 gctctatcct ctgagctacg agggcaggag actgggaagc attgtcccac aacggccgag
     7621 gtgtgtcaaa cgggcggatg accccggcgc gccgacctga caggtaggca gccatgcatg
     7681 ggactgccac cagagaacga ctggtcgtcc acctgtcagg tcaatggtcg ccagacgact
     7741 ttggaaaagc cct
//
LOCUS       DAOSMQ010000089         7730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9429_length_7730_cov_4.665798, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000089 DAOSMQ010000000
VERSION     DAOSMQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9429_length_7730_cov_4.665798"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PK829_04420"
     CDS             <1..892
                     /locus_tag="PK829_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU40495.1"
                     /translation="QALSADTGLPKERLLAGMGADELIDLVLRALLEPGDAVLNCPPT
                     FGMYPFSTAVNGGRTVAVPRDPDFALDVDGIIAAASAEPRAKVLFLCSPNNPDGGVID
                     DEALRRLLALPLLVVLDEAYVDFAIAEGMPGRLHWVMDYDNLAVLRTFSKLAGLAGLR
                     LGYGAFPEWLLPYLWKIKPPYNVNVAAALAAVAALAEREWLADRVGRLVAERARIARE
                     AAALGWLRPFPSRANFVLCRVDGMPAAALQRALAAEGILVRHFDKPGLRDCIRISAGR
                     PEDTDRLLAALRRVAPRSEG"
     gene            889..1485
                     /gene="hisB"
                     /locus_tag="PK829_04425"
     CDS             889..1485
                     /gene="hisB"
                     /locus_tag="PK829_04425"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOU40496.1"
                     /translation="MSKRQATVHRQTSETDVRVTIDLDGRGAHRVATGVGFLDHVLAA
                     MAVHGLFDITVEATGDLHIDAHHTIEDTAIVLGQAVAVALGDRAGIHRAGHAYMPMDE
                     ALAFVALDVSGRPYTVFRATWYTPAIGAFPTDLVEHFFESFAVHARLTLHARLEVGRN
                     DHHGAEALFKALARALAVAVERDPRRAGVASTKGTLSS"
     gene            1509..2138
                     /gene="hisH"
                     /locus_tag="PK829_04430"
     CDS             1509..2138
                     /gene="hisH"
                     /locus_tag="PK829_04430"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOU40497.1"
                     /translation="MLTMIDYGGSNLRSAQKALEAAGARVHVTDDPAVVRTAARLVLP
                     GVGAFGAGMAALRARGLDGATVAAVAAGATLLGICLGMQFLFDESEEMGRHAGLGLLA
                     GRVARFPAGLTHDGRPLKVPHVGWNEIEPVGAPPLLDGIGPAAHAYFVHSFYCAPADA
                     ADVLAYADYGGRFAALVGRGRVFGYQFHPEKSQRVGLRLLRNYITLEAT"
     gene            2135..2890
                     /locus_tag="PK829_04435"
     CDS             2135..2890
                     /locus_tag="PK829_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOU40498.1"
                     /translation="MTGFDIYPAIDLRRGRVVRLELGDPARETVFGDNPAAMAGRWRD
                     AGARWLHVVNLDGAFAEAGAANWAALAAIVAAGGVRVQFGGGLRTLDDIAAALDSGAA
                     RVVLGTAALASPELLAAAVARFGPERVAAGIDARDGRVRVRGWQTETAVTPAALALSA
                     RAAGVTTVITTDIARDGVLTGVNAEATAALAAASGLAVIASGGARDLGDVARLLALAP
                     TAAPGRLAGVILGRALYDGHIDLAAALARAEAG"
     gene            2900..3664
                     /gene="hisF"
                     /locus_tag="PK829_04440"
     CDS             2900..3664
                     /gene="hisF"
                     /locus_tag="PK829_04440"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOU40499.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVNFIDLRDAGDPVAQARFYDAEGA
                     DELVFLDITATHEARGTVMAMARAVADQVFIPFTVGGGIRSVDDMRALLRAGADKISI
                     NSAAVRVPQLIAAGATEFGSQAVVVAIDARRRANGAGWEVYVSGGRAPTGLDAVQWAC
                     QAEALGAGELLLTSMDGDGTQAGYDVALTRAVAEAVAIPVIASGGAGTPAHFHAVLTD
                     GRADAALAASLFHYRQLSVGQVKDYLSARDVAVRRA"
     gene            3664..4304
                     /gene="hisIE"
                     /locus_tag="PK829_04445"
                     /pseudo
     CDS             3664..4304
                     /gene="hisIE"
                     /locus_tag="PK829_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146378.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
     gene            4346..7204
                     /locus_tag="PK829_04450"
     CDS             4346..7204
                     /locus_tag="PK829_04450"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU40500.1"
                     /translation="MPRPVDYLPGAAVGLFAARVLGEAIGLPGVWAAGLLGALLAGGG
                     AWLLARWPLGRTWPALLLLAYVVWPEVNPAARLWLPGVAVVVLLLHERARGAGGERWS
                     LTDRRLTLAISAALFVAALALYVATLAPDVLAADSGELQTVAAQLGVAHPPGFPLYVL
                     LAHLFTRLPVGSPAHAVNLLSAVFAALTVVVVYRTGLLLAGRHLAALVGALALGTATT
                     FWSQATTANVRSLTALFAALFIYALVCFGIATSNGSAGMGHGGRPIRQNTTPADRALI
                     WAALFIGFGLTHHVSLVFLAAVGLVFVVVVDRSLLRSPRRWGRPLMAALLGLLPLLYL
                     PLRAGADARGASPQLATLPGFLEHVLATGFRGDLFYYLAPADLTQRLGIMGNVMTFQF
                     APLLLAGMVVGLLLLLWRDRPMAWLLGGSFAVYVFVAATYRAPQTVEYMMPAYVPAAL
                     AVAVALGRWPLARPRWRGPGPALGALFAALMLAAALSQLPGHISAAGARHEAGDARAV
                     ATRLLAEAPPNAIILAHWHWATPLWYLREVEGLRPDVDVRFVFPEGETYEATWARRTA
                     AAFAEGRPVVTTWLPAAPLANLPVPEPLGEALLYPQTPRTALPAGYETVDLSLGESIA
                     VVGYRLETNGAFAPGEEAVLTLAWRPRAELSAGLGLYAHLVAAGGALYAQDDRPALAA
                     AGLTLTQFRLTPRPATPAGALTLFVGASDGGERQALVMLPLGPGGARPFTRNPVFRAA
                     TDGLAGTLIGYDWDHTIAGRARLYLHWRADDGYTTQAFDDAAPALLLPYRGPWGVPVR
                     DWRFPRGQADGHYVPLGQGIVWTGETLDGLSLAPGAPVVLGQRFHSGRPVNRDLVVSV
                     RLIGLEPDGFHWAWWDLRDSVPALGAIPTLKWVAGSAVRSPHRVTAATGAPPGQALTG
                     ALTLYDAFTGRPLPILDERIPAGQPWIPLGQGVVVGQE"
     gene            complement(7230..7301)
                     /locus_tag="PK829_04455"
     tRNA            complement(7230..7301)
                     /locus_tag="PK829_04455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7267..7269),aa:Ile,seq:cat)
     gene            complement(7395..7465)
                     /locus_tag="PK829_04460"
     tRNA            complement(7395..7465)
                     /locus_tag="PK829_04460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7431..7433),aa:Gly,seq:tcc)
     gene            complement(7473..7546)
                     /locus_tag="PK829_04465"
     tRNA            complement(7473..7546)
                     /locus_tag="PK829_04465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7510..7512),aa:Pro,seq:tgg)
BASE COUNT          988 a         2723 c         2792 g         1227 t
ORIGIN      
        1 ccaggccctc agcgccgaca ctggcctgcc gaaagagcgc ctgctggcgg gcatgggcgc
       61 ggacgaactg atcgacctcg tgctgcgggc gctgctggag ccaggcgacg ccgtgctcaa
      121 ctgcccgccg acgttcggca tgtacccctt ctcgacggcg gtcaacggcg gccggacggt
      181 ggccgtgccg cgtgacccgg acttcgccct cgacgttgat ggcattatcg ccgccgcgtc
      241 ggccgagccg cgggccaaag tgctcttcct ctgctcgccc aacaaccccg atggcggcgt
      301 catcgacgac gaggcgttgc gccggctgct ggccctgccg ctgctggtcg tcctcgacga
      361 ggcctacgtc gatttcgcca ttgccgaggg catgcccggc cggcttcact gggtgatgga
      421 ctacgataac ctcgccgtcc tgcgcacctt cagcaagctg gccgggctgg ccgggttgcg
      481 cctgggctat ggggctttcc cggagtggct gttgccctac ctgtggaaga tcaagccgcc
      541 ctacaacgtc aacgtcgccg ccgcgctggc cgccgtggcc gcgctggcgg agcgagagtg
      601 gctggcggac agggtcggcc gcctggtggc cgagcgcgcg cgcatcgccc gcgaggcggc
      661 ggctctgggc tggctgcggc cgtttcccag ccgggccaac ttcgtcctct gccgcgtgga
      721 cggcatgccc gcggcggcgc tgcagcgcgc cctggcggcc gagggcatcc tcgtgcgcca
      781 cttcgacaag cccggcctgc gcgactgcat ccgcatcagc gccggacggc cggaagacac
      841 cgatcgatta ctggcggcgc tgcggcgcgt cgcccccagg agcgaaggat gagcaaacga
      901 caggcgaccg ttcaccggca gacaagcgag accgacgtgc gcgtgaccat cgacctcgac
      961 ggccgcggcg cgcaccgcgt ggccaccggc gtgggcttcc tcgaccacgt gctggcggcg
     1021 atggccgtcc acgggctgtt cgacatcacc gtcgaggcca cgggcgacct ccacatcgac
     1081 gcccaccaca ccatcgagga cacggccatc gtcctggggc aggccgtcgc cgtggcgctg
     1141 ggcgaccggg cgggtattca ccgcgccggc cacgcctaca tgcccatgga cgaggccctg
     1201 gccttcgtcg ctctcgatgt cagcggccgg ccctacaccg tcttccgggc cacgtggtac
     1261 acgccggcca tcggcgcgtt tccgaccgac ctggtggagc acttcttcga gtcgttcgct
     1321 gtccacgccc gcctgacgct gcacgcgcgg ctggaagttg gccgcaacga ccaccacggc
     1381 gcggaggcgc tcttcaaagc cctggcgcgg gcgctggccg tcgccgtcga gcgcgacccg
     1441 cgccgcgccg gcgtcgcctc gaccaagggc acgctgtcgt catgaccgcg tcctcggccg
     1501 gcgggccgct gctcacgatg atcgactacg gcggcagcaa cctgcgctcg gcccagaagg
     1561 cgctggaggc ggccggcgcg cgcgtccacg tgacggacga ccccgccgtg gtgcggacgg
     1621 cggcgcgact ggtgttgccc ggcgtgggcg cgttcggcgc gggcatggcc gccctgcggg
     1681 cgcgcggtct ggacggggcg acggtggccg ccgtggccgc tggggcgacg ctgctgggca
     1741 tctgcctggg gatgcagttc ctttttgacg agagcgagga gatggggcgg cacgccgggc
     1801 tggggctgct ggccgggcgg gtagcgcgct ttcccgccgg cctgacccac gacggccggc
     1861 cgctgaaagt gccccacgtc ggctggaacg agatcgagcc ggtcggcgct ccgccgctac
     1921 tcgacggcat cggcccggcg gcgcacgctt acttcgtcca ctcgttctat tgcgccccgg
     1981 ccgacgccgc cgacgtgctg gcctatgccg actacggcgg gcgcttcgcc gccctcgtcg
     2041 gccgcgggcg cgtgttcggc taccagttcc acccggagaa gagccagcgc gtgggcctgc
     2101 gcctgctgcg caattacatc actctggagg cgacatgacc ggcttcgaca tttacccggc
     2161 catcgatttg cgccgcggcc gtgtcgtgcg gctagagctg ggcgaccccg cccgcgagac
     2221 cgtcttcggc gacaacccgg cggctatggc cggccgctgg cgcgacgccg gcgcgcgctg
     2281 gctccacgtc gtcaatctcg acggcgcgtt tgccgaggcg ggcgcggcca actgggccgc
     2341 cctggcggcc atcgtcgccg cgggcggggt gcgcgtgcag ttcggcggcg gcctgcgcac
     2401 gctcgacgac atcgccgcgg cgctggatag tggcgcggcc cgcgttgtgc tgggcacggc
     2461 cgcgctggcg tcgccggaac tactggccgc ggccgtggcc cgttttggcc cggagcgcgt
     2521 ggccgccggc atcgacgctc gcgacggccg cgtgcgcgtg cgcggctggc aaacggagac
     2581 ggccgtcacg ccggcagccc tggccctcag cgctcgcgcc gccggcgtaa cgacggtcat
     2641 caccaccgac atcgcccgcg acggcgtgct gaccggcgtc aacgcggagg ccacggcggc
     2701 gctggccgcg gccagcgggc tggccgtcat cgccagcggc ggcgcgcgcg acctgggcga
     2761 cgtggcgcgg ctgctggcgc tggcgccaac ggccgcgccc ggccggctgg ctggcgtcat
     2821 cctcggccgc gccctctacg acggccacat cgacctggcc gcggcgctgg cccgcgccga
     2881 agcgggttag gaggcgagca tgttggccaa acgcatcatt ccctgtctgg acgtgaagga
     2941 cggccgcgtc gtcaaaggcg tcaacttcat cgacctgcgc gacgccggcg acccggtggc
     3001 tcaggcgcgc ttctacgacg ccgagggagc cgacgaactg gtcttcctcg acatcaccgc
     3061 cacccacgag gcccgcggca cggtcatggc catggcccgt gcggtggccg accaggtctt
     3121 catcccgttc accgtcggcg gcggcatccg cagcgtggac gacatgcgcg cactgctgcg
     3181 ggccggggca gacaagatca gcatcaactc ggccgccgtg cgcgtcccgc agctgatcgc
     3241 cgccggggcg acggagttcg gctcgcaggc ggtcgttgtg gccatcgacg cgcggcggcg
     3301 agcgaacggg gccggctggg aggtatacgt cagcggcggg cgcgccccga cggggctgga
     3361 tgccgtccaa tgggcgtgcc aggccgaggc gctgggcgcg ggcgagttgt tgctgacgag
     3421 catggacggc gacggcacgc aggcgggtta cgacgtggcc ctgacgcggg ccgtggccga
     3481 ggccgtggcc atcccggtga tcgcttccgg cggcgcgggc acgccggccc acttccacgc
     3541 cgtgctgacc gacggccggg ccgacgcggc tctggcggcg tcgctgttcc actaccggca
     3601 gctcagcgtc ggccaggtga aggactatct gagcgcgcgc gacgtggccg tgcggagggc
     3661 ctgatggaca ccgaactgcg ctacgacgcc cgtggcctca tccccgccgt cgtccaggac
     3721 gcgacgacga acgcggtgct catggtcgcc tggatgaacg ccgaagcgct gcgccggacg
     3781 caggccaccg gcgagaccca cttctggagc cgcagtcgcg atgagttgtg gcacaagggg
     3841 gcgacatcgg gcaatgtaca gcgcgtccgc gaaatccgct atgattgcga cggcgacacg
     3901 ctgctgctgc tggttgaccc ggccggcccg gcctgccaca ccggcgcggc gtcgtgcttt
     3961 taccggatgc tggccgttgg gcaagccggg gacgaaggca tatgatgagt gatatggatg
     4021 tgggtgtggg tgtcatccgc gaactggaag ccacgctgcg cgagcgcgcc ggccgcccgc
     4081 cggccgagtc ctacaccgcc cggcttctgg ccgccggcga agacgagatc gtcaagaagg
     4141 tcggcgaaga ggccatcgag gtcatcctgg ccgtcaaggg gcagggggat gagcgactgg
     4201 tcagcgagac ggccgatctg gtctatcacc tgctcgtcct gctgcaatcg cgcggcctgg
     4261 actgggccgc cgtcgaggcc gaactggccc gccgccgcaa gtaggagagc ctccgctgag
     4321 cactgccgcg ccatcgcccc gccgtttgcc tcgtcccgtc gattatctgc ccggcgcggc
     4381 cgtgggtttg ttcgccgccc gcgtgctggg cgaggccatt ggcttgccgg gcgtctgggc
     4441 ggccgggctg ctgggcgcgt tgttggccgg cggcggggcg tggttgctgg cccgctggcc
     4501 gctgggccgc acgtggccgg cgctgttgct gctggcctac gtcgtctggc cggaggtgaa
     4561 cccggcggcg cggctgtggc tgccgggcgt ggctgtggtc gtcctgctgc tgcatgagcg
     4621 ggcgcgcggg gccggcggcg agcgctggtc gctgactgat cgccgcctga ctttggccat
     4681 cagcgcggcc ctgttcgtcg ccgcgctggc gctctacgtg gccacgctgg ccccggacgt
     4741 gctggccgcc gacagcggcg aattgcagac cgtggccgcg caactgggcg tggctcatcc
     4801 acccggtttc ccgctgtacg tcctgctggc ccacctgttc acgcggctgc cagtcggctc
     4861 gccggcccat gccgtcaacc tgctctcggc cgttttcgcc gcgctgaccg tcgtggtcgt
     4921 ctaccggacg gggctgctgc tggcggggcg acacctggcc gcgctggtgg gcgcgctggc
     4981 gctgggcacg gcgacgacct tctggtcgca ggcgacgacg gccaacgtgc gcagcctgac
     5041 ggcgctgttc gcggcgctgt tcatttatgc gttggtgtgc ttcgggatag cgacgagcaa
     5101 tggcagtgct ggaatgggcc acggaggccg gcccatccga cagaacacga ccccggccga
     5161 ccgcgccctt atctgggccg ccctcttcat cggcttcggc ctgacccacc acgtctcgct
     5221 tgtctttctg gccgcggtgg ggctggtctt cgtcgtcgtt gtcgatcggt cgttgctgcg
     5281 gtcgccgcgg cgatgggggc ggccactgat ggcggcgctg ctggggctgc tgcccttgct
     5341 ctatctgccc ttgcgcgccg gggcggatgc gcgcggcgcg tcgccgcagc tggccacgct
     5401 gcccggcttc ctggagcatg tcctggcgac cggtttccgc ggcgatctgt tctattacct
     5461 ggccccggcc gacctgaccc agcgcctggg catcatgggc aacgtcatga ccttccagtt
     5521 cgcgccgctg ctgttggcgg gcatggtcgt gggcctgctc ttgctgctgt ggcgcgaccg
     5581 gccgatggcc tggctgctgg gtgggtcgtt cgcggtctac gttttcgtcg ccgccaccta
     5641 ccgcgctccg cagacggtcg agtacatgat gcctgcctat gtgcccgccg cgctggcggt
     5701 ggccgtcgcc ctcggccggt ggccgttggc gcggccgcgt tggcgcgggc cggggccggc
     5761 gctgggcgcg ctcttcgccg cgctgatgct cgccgccgct ctgagccagc tacccggcca
     5821 catctccgcc gccggggcgc gccacgaggc cggcgacgcc cgcgccgtgg ccacccgcct
     5881 gctggctgag gccccgccca acgccatcat cctggcccac tggcactggg ccacgccgct
     5941 gtggtacctg cgcgaggtcg aagggctgcg gccggacgtg gacgtgcgct tcgtcttccc
     6001 ggagggcgag acctatgagg ccacctgggc gcggcggacg gcggcggcct ttgccgaggg
     6061 gcggccggtc gtcaccacct ggctgccggc cgcgccgctg gccaacctgc ccgtgccgga
     6121 gccgctgggc gaggcgctgc tgtacccgca aacgccgcgc acggccctgc cggccggcta
     6181 cgagacagtc gatctgtcgc tgggcgagag tatcgcggtc gtgggctacc ggctggagac
     6241 caacggcgcg ttcgcgcccg gcgaagaggc cgtgctgacg ctggcctggc ggccgcgcgc
     6301 ggagctatcg gccgggctgg gtctctacgc ccacctcgtc gctgccggcg gcgcgctcta
     6361 cgctcaggac gaccgcccgg cgctggccgc cgcggggcta acgctgacgc agtttcggct
     6421 gacgccgcgc ccggccacgc ccgccggcgc gctgaccctc ttcgtcggcg cgagcgacgg
     6481 cggcgagcgg caggcgttgg tcatgctgcc gcttgggcct ggcggcgcgc gccccttcac
     6541 gcgcaatccg gtcttccggg ccgcaaccga cgggctggca ggcacgctca tcggctacga
     6601 ctgggatcac accatcgccg gccgcgctcg cctctatctc cactggcgcg ctgatgacgg
     6661 ctacacgacg caggcgttcg acgacgccgc gccggcccta ttgctgccct atcgcgggcc
     6721 gtggggcgtg ccggtacgcg actggcgctt cccgcgcggg caggcggacg gccattacgt
     6781 ccccctgggg cagggcattg tctggacggg agagacgctc gacggcctat cgcttgcgcc
     6841 gggcgcgccg gttgtcctcg gccagcgctt ccacagcggc cggccggtca accgcgacct
     6901 tgttgtctcc gtgcgcctca tcggcctgga gccggacggc ttccactggg cctggtggga
     6961 tctgcgcgac agcgtgccgg cgctgggggc cattccgacg ctgaagtggg tggcggggtc
     7021 ggccgtgcgc tcgccccatc gcgtcacggc ggccaccggc gcgccgccgg ggcaggcgct
     7081 gaccggggcg ctgacgctct acgacgcctt caccggccgg ccgctgccga tcctcgacga
     7141 gcgcatcccc gccgggcagc cgtggatacc gctggggcag ggcgtggtcg ttggacagga
     7201 atgagggcgg gcgcgcgttg gatggggaat gggcctgcta ggattcgaac ctagaaccaa
     7261 tcggttatga gccgaccgct ctgccgttga gctacaggcc cggttcagtg tccagtattc
     7321 agtaggtcag tgttcagtgc gacgagctag taagactgag tactggatac tgaactactg
     7381 aatactgatt caagagcggc agacgggatt cgaacccgtg aaaacagctt ggaaggctgt
     7441 tgtgttacca ctacaccact gccgcagagt agtcggggcg gccagacttg aactggcggc
     7501 ctcgcggtcc caaaccgcgc gctctagcca actgagctac accccgcgac agaggcggaa
     7561 gcaccagtaa taaataaaat acagcaggat tataacaggt ttaattgcta agaacccatg
     7621 cttcaatatt aaaatccttt acatccgaag cataagttct caccttgtta cttatcaaat
     7681 ccacaaggtg tgttttaaag gtatgtttgc aagtctcttc tctggtgcag
//
LOCUS       DAOSMQ010000090         7678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9528_length_7678_cov_4.647514, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000090 DAOSMQ010000000
VERSION     DAOSMQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9528_length_7678_cov_4.647514"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1151
                     /locus_tag="PK829_04470"
     CDS             246..1151
                     /locus_tag="PK829_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40501.1"
                     /translation="MATVDAPITLATGADELAPPLTFRQMAWRRFRRHRMAMAGAVIL
                     ILLFVYAFGGGLIFTAERANFTDTSARLQGPSAAHPFGTDTVGRDVLARTIQGGRISL
                     MIGLMAAVLEIVIGVVVGAVAGYYGGIVDSLLMRLTEAMLIIPSLFLLIVAARLVGGS
                     VTWIIIIFALTSWMYVARIVRAEFLSLKENEFILAARSIGTPTSQIIFRHILPNSMAP
                     IVVSATLAVATAILSEAYISFLGMGVQPPTATWGNMLDGSYNYIESAPWLWIFPGLLI
                     LLTVLSINFVGDGLRDALDPRSRNV"
     gene            1151..2191
                     /locus_tag="PK829_04475"
     CDS             1151..2191
                     /locus_tag="PK829_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40502.1"
                     /translation="MTASSSRPTAAPRPLLLDIRNLQVRFHTPEGTVYAVNDISYKVH
                     EGEVIAVVGESGCGKSVSMMAVLGLIPRPPGEIAGGEALFMGRDLLRLPEDDMQSIRG
                     GDIAMVFQDPMTSLNPVLSVRRQMTESLRRHANMSPEQARDRAIELLELVGIPDAARR
                     LGDYPHQFSGGMRQRVMIAMMLACNPSLLIADEPTTALDVTIQAQIVELVTRMREKLG
                     MALIWITHDLGVVAGLAERVIVMYAGMIVEEADVDSLFDQPRHPYTLALLGALPRIDR
                     RRDSRLNSIPGAPPNLLVEPRGCPFAPRCEYVIDHCRVERPPLMAVSPTQWSACWVMA
                     GGEGNYELGIRN"
     gene            2327..3355
                     /locus_tag="PK829_04480"
     CDS             2327..3355
                     /locus_tag="PK829_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU40503.1"
                     /translation="MTDDERPTSNGEPLVRVVELTKHFPIRQGLIFQRVTGQVRAVDG
                     ISFEIARGETLGLVGESGCGKSTAGRTLLGLYPATSGASFIAGHHVQTATGDEWLAIR
                     RGAQMIFQDPFASLNPRWTVNGIISEPLRVHNLVDGEAARTERVKELMALVGLDGRYI
                     NRFPHEFSGGQRQRIGIARSLASDPLFLVCDEPISALDVSIQAQVVNLLEELQDRLGL
                     TYLFIAHDLSMVRHICDRVVVMYLGVMVESAAKDDLYEKPLHPYTQALLSAVPVPDPK
                     LARRRQRIVLEGDVPSPINPPSGCRFHTRCPIAQPHCTTHVPVWREIDPGHWVACHEV
                     EPKYVPNQ"
     gene            3352..5202
                     /locus_tag="PK829_04485"
     CDS             3352..5202
                     /locus_tag="PK829_04485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU40504.1"
                     /translation="MTNRVIVAAKYERHGWPSVDRPDLKPPDGWSLALVNSVNLIHHH
                     ELSPDGQQVAFVWERDGRADIFTQALSGGWPRRVSTDRAKTIYWWDSPPRWSPDGRWL
                     AFTQGGHVHVAPADGRGQSRAVTDFTAGASSPTWLPDSNGLIVAAERGEGTLLLLTDR
                     DGRWPRPLAEDAGDNLDAAPSPDGGRVAYIHRPLDDLNRWDLRLVDIASGQTTTLFGL
                     PREKAWSPRWSPDGQQIAFLSQRSGYTEVWLVRPDGDGPRQLTRLSRDVTEFAFSPDG
                     RTLAATVSREGAIDLLLADAGSGAARALRTGKGCYATPQWSPDGRFLTVEYEDATTPA
                     DIYRLEVDSGRMTPLTYSLPPALAANDRITPEHVRYPSFDGLEIPALLWRPARPNGAA
                     IVRPHGGPADATRFQWDALAQYFVAKGYTYLAPNFRGSAGYGRAFEHANYNNWGVGDT
                     KDVLHGARYLHSLGVDRERVGILGSSYGGYMVACCLARDPDYLFACGVSKYGDANVYS
                     SWAQCERGTRLYTEMMLGHPRDNWAVYRAASPIDEMANVRAPILLLHGLEDDVVPPQA
                     SEEWAEALRRHDKVFEYVTYAGEPHGFLRYETEMDWQRRAERFFDWYLRA"
     assembly_gap    5266..5365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5483..7363)
                     /gene="mutL"
                     /locus_tag="PK829_04490"
     CDS             complement(5483..7363)
                     /gene="mutL"
                     /locus_tag="PK829_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU40505.1"
                     /translation="MIHVLPDQLASQIAAGEVVERPSSVVKELVENSIDAGATAITVE
                     IRGGGRQLIQVADNGSGIDAGEVETAFLRHATSKLNTADDLHAIRTLGFRGEALAAIA
                     AVSQVTVVSRAREATAGTRLVLDGGHIMTRETAGAPVGTVIAVENLFYNVPARLKFLK
                     SITTEKRLIDEFVTRYALAYPGIRFRLSHDNRITFQTTGNGETAGVLLAVYGPETVRQ
                     LITIPADEGDERRVGVRGYVSDPALNWANRNHIQLFVNGRAVRDNRLTFAVVQAYHTL
                     LPVGRYPLGLLFLDVPVEDVDVNVHPAKVEVRFRDEGTMFSAVQREVRAALLGTTPGR
                     AAGGWPAGDSLGGWSSWGGRPAFAPRKDDGDRPAAQLDIPWQLRPPAPESELDALDGD
                     NPGDGDNPGDGDNPGDGDNPGDGDNAGVEPAVAPPPLIPHEPRLPIMRVIGQVGAAYI
                     IAEGPEGLYLIDQHAAHERILFEQLLAQWRQRRVASQGLVAPATVYLPPSQAALVDEQ
                     AGLLAGLGFEIEPFGPGAFQVRAVPALLGRTDPATALMAVVSDLENEVAPLGAQLEAQ
                     VIKRVCKSAAVKAGQTLSRDEMEALIAQLETCETPHTCPHGRPTLIHISAAQLARQFG
                     RT"
BASE COUNT         1173 a         2647 c         2518 g         1240 t
ORIGIN      
        1 tcctcgacca catgcagcgc ttcgacacgc cggtggtcat gggcattctc atcctgctgt
       61 cggtgacggt gatcgtcttc cagatcatca ccgacctgac ctatgcgctg ttggacccga
      121 ggattcgcta tgactaggtc tcttgactat ctgtggggga gcgggggggc aggagagcgg
      181 gggagagaag aatcgccccg gctcccctac ccccgtgccc ccctgccttc ttacatggac
      241 ggcggatggc cacagtagac gcccccatca ccctcgctac cggcgcggac gagctggccc
      301 cgccactgac cttccggcag atggcctggc ggcgcttccg gcggcaccgc atggccatgg
      361 ccggcgcggt catcctcatc ctgctgtttg tctacgcttt cggcggcggc ctgatcttta
      421 ccgccgagcg ggccaacttc accgacacca gtgcccggct gcaaggcccc tcggccgccc
      481 atcccttcgg cacggacacc gtcggccgcg acgtgctggc ccgcaccatc cagggcgggc
      541 gcatctcgct gatgatcggc ctcatggccg ccgtgctgga gatcgtcatc ggcgtggtcg
      601 tcggcgcggt ggccggctac tacggcggca tcgtagatag cctgctcatg cgtctgactg
      661 aggccatgct gatcatcccg tcgctgttcc tgctcatcgt cgccgcgcgg ctggtgggcg
      721 gcagcgtgac gtggattatc atcatcttcg ccctgacgag ctggatgtac gtcgcccgca
      781 tcgtccgggc cgagttcctg tcgctgaagg agaacgagtt catcctggcg gcgcgttcca
      841 tcggtacgcc gacgagccag atcatcttcc gccacatcct gcccaacagc atggccccca
      901 tcgtcgtctc ggccacgctg gccgtggcca cggccatcct ctcggaggcg tacatcagct
      961 tcctgggcat gggcgtccag ccgccaacag ccacgtgggg caacatgctg gacggatcgt
     1021 acaactacat tgaatccgcg ccgtggctgt ggattttccc cggcctgctg atcctgctga
     1081 cggtgctcag catcaacttt gtcggcgacg gcctgcgcga cgccctcgat ccccggagcc
     1141 ggaacgtcta atgactgcat cctccagccg ccctacagcc gcgccccgtc ccctgctgct
     1201 ggacatccgc aacctgcaag tccgcttcca cacgcccgaa ggcacggtct acgccgtcaa
     1261 cgacatctcc tacaaggtgc atgagggcga ggtcattgcc gtggtgggcg agagtggctg
     1321 tggcaagtcg gtcagcatga tggccgtgct ggggctgatt ccccggccgc cgggggagat
     1381 cgccggcggc gaggcgctct tcatgggccg cgacctgctg cggctgccgg aagacgacat
     1441 gcagtccatc cgcggcggcg acatcgccat ggtcttccag gacccgatga cctcgctcaa
     1501 ccccgtgctc agcgtccggc ggcagatgac cgagtcgctg cgccgccacg ccaatatgtc
     1561 gccggagcaa gcccgcgacc gggccatcga gctgctggag ctggtcggca tccccgacgc
     1621 ggcccggcgg ctgggcgact acccgcacca gttctccggc ggtatgcgcc agcgagtgat
     1681 gatcgccatg atgctggcct gcaaccccag cctgctcatc gccgacgagc cgacgacggc
     1741 cctcgacgtg accatccagg cgcagatcgt cgaactggtg acgcggatgc gcgagaagct
     1801 gggcatggcc ctcatctgga tcacccatga cctgggcgtg gtggccgggc tggccgagcg
     1861 cgtcatcgtc atgtacgccg gtatgatcgt cgaagaggcg gacgtggata gcctgttcga
     1921 tcagcccagg catccctaca cgctggccct gctgggggcg ctgccgcgca tcgaccggcg
     1981 gcgcgacagc cggctcaact ccatccccgg cgcgccgccc aacctgctgg tggagccgcg
     2041 cggctgcccc ttcgcgccac gctgcgagta tgtcatcgac cactgccgcg tcgaacggcc
     2101 gccgctgatg gccgtctcgc cgacgcaatg gtcggcgtgc tgggtgatgg ccgggggaga
     2161 ggggaattac gaattaggga ttaggaatta ggaatgaaga ggaggaagag ctcacgccaa
     2221 ggacgccagg gaagcaaagg acgccaagaa gaaagcctcc ttgtcttctt ggcgctcttt
     2281 gcccccttgg cgtccttggc gtgagctctt ctcgcataag cgacagatga ccgacgacga
     2341 acgacccacg agtaacggcg agccgctagt gcgcgtcgtg gagctaacca agcacttccc
     2401 catccgccag gggctgatct tccagcgcgt caccgggcag gtgcgggcgg tggacggcat
     2461 ctcctttgag atcgcccgcg gcgagacact ggggctggtg ggcgagagcg gctgcggcaa
     2521 gtccacggcc gggcggacgc tgctgggcct ctacccggcc accagcggcg ccagcttcat
     2581 cgccggccat cacgtccaga cggccacggg cgacgagtgg ctggccatcc gccgcggggc
     2641 gcagatgatc ttccaggacc ccttcgcctc gctcaacccg cgctggacag tcaacggcat
     2701 catctccgag ccgctgcgcg tccacaacct cgtggacggc gaggcggcgc ggacggagcg
     2761 cgtcaaggag ttgatggccc tggtagggct ggacggccgc tatatcaacc gcttccccca
     2821 cgagttctcc ggcggccagc gccagcgcat cggcatcgcc cgctcgctgg cctccgaccc
     2881 gctcttcctg gtttgcgacg agccgatttc cgccctggac gtgtccatcc aggcgcaggt
     2941 cgtcaatctg ctggaggagc tgcaagaccg gctggggctg acctacctgt tcattgccca
     3001 cgacctgagc atggtgcgcc acatctgcga ccgggtggtg gtcatgtacc tgggcgtcat
     3061 ggtcgagtcg gcggccaagg acgacctgta tgagaagccc ctgcacccgt acacgcaggc
     3121 gctgctgtcg gccgtgcccg tgcccgaccc caagctggcc cgccgccgcc agcgcatcgt
     3181 cctggagggc gacgtgccca gccccatcaa tccgccgtcg ggctgccgct tccacacccg
     3241 ctgccccatc gcccaaccgc actgcaccac ccacgtgccc gtctggcggg agattgaccc
     3301 cggccactgg gtggcgtgtc atgaggtgga accaaaatac gtgcccaatc aatgacaaat
     3361 agagtcattg tcgccgcgaa gtatgagcgc cacggctggc cctccgtcga ccggcccgac
     3421 ctgaagccgc ccgacggctg gagcttggcg ctcgtcaact cggtcaacct catccaccac
     3481 cacgagttgt cgcccgacgg gcagcaggtg gcttttgtct gggagcgcga cggccgcgcc
     3541 gacatcttta cgcaagccct gtccggcggt tggccgcgac gtgtctcgac tgaccgggcc
     3601 aaaacgatct actggtggga cagcccgccg cgctggtcgc ccgacggccg ctggctggcc
     3661 ttcacccagg gcggccatgt ccacgtcgcg ccggccgacg gccgcggcca gtcccgcgcc
     3721 gtcaccgact tcacggccgg cgcttcgtcg cccacctggc tgcccgatag caacggcctg
     3781 atcgtcgccg ctgagcgggg cgagggcacg ctgctgctgc tgaccgatcg cgacggccgt
     3841 tggccgcgcc ccctggccga ggacgccggc gacaacctcg acgccgcccc ttcgcccgac
     3901 ggcggtcggg tcgcctacat ccatcgcccg cttgacgacc tgaaccgctg ggatttgcgc
     3961 ctggttgaca tcgccagtgg gcagacaacg acgctcttcg gcttgcccag ggagaaagcc
     4021 tggtcgccgc gctggtcgcc cgacgggcag cagatcgcct tcctgtcgca gcgcagcggc
     4081 tacaccgagg tctggctcgt ccggcccgac ggcgacgggc cgcgccagct cacccgcctg
     4141 agccgcgacg tgaccgagtt cgctttctcg cccgacggcc ggacgctggc agcgacggtc
     4201 agccgcgagg gagccattga cctgctgctg gcggacgccg gcagcggcgc ggcgcgcgcg
     4261 ctgcggaccg gcaagggctg ctacgccacg ccgcaatggt cgcccgacgg gcgcttcctg
     4321 accgtcgagt atgaggacgc gacgaccccg gccgacatct accggctgga ggtagacagc
     4381 gggcgcatga cgccgctgac ctactcgctg ccgccggcgc tggccgccaa cgaccgtatc
     4441 actccggagc acgtgcgcta ccccagcttc gacgggctgg agattccggc gctgctgtgg
     4501 cggccggcgc ggcccaacgg cgccgccatc gtccgccccc acggcggccc ggccgatgcc
     4561 acccgcttcc agtgggacgc gctggcgcag tacttcgtcg ccaagggcta cacctacctg
     4621 gcccccaact tccgcggcag tgccggctac ggccgcgcct tcgagcacgc caactacaac
     4681 aactggggcg tcggcgatac caaggacgta ctccacgggg cgcgctatct ccacagcctc
     4741 ggcgtcgacc gggagcgcgt cggcatcctc ggcagcagct acggcggcta tatggtcgcc
     4801 tgctgcctgg cccgcgaccc cgactatttg ttcgcctgtg gcgtcagcaa gtacggcgat
     4861 gccaatgtct atagttcctg ggcgcaatgc gagcggggca cgcgcctgta cacagagatg
     4921 atgctcggcc acccgcgcga caactgggcc gtctaccgtg ccgcctcgcc catcgacgag
     4981 atggccaacg tccgcgcgcc catcctgctg ctccacgggc tggaggatga tgtcgtccca
     5041 cctcaggcat ccgaggaatg ggccgaggca ttgcgccggc atgacaaggt cttcgagtac
     5101 gtcacctacg ccggcgagcc acacggcttc ctgcgttatg agacggagat ggactggcag
     5161 cggcgggcag agcggttctt cgactggtat ctgcgcgcgt agcgtggcgc gattctcccg
     5221 aatcgcgtag cgtggcgcga ttctcccgaa tcgcgtagcg tggcgnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnngattc tcccgaatcg cgtagcgtgg cgcgattctc
     5401 ccgaatcgcg tagcgtggcg cgattctcct gaatcgcgat gcgccctggc gaagaaccgc
     5461 gattcgggag aatcgcgcca cgttatgtcc ggccgaattg gcgggccaac tgcgcggcgg
     5521 agatgtggat gagcgtcggc cggccgtggg ggcaggtgtg gggcgtttcg cacgtctcta
     5581 gctgggcgat gagcgcctcc atctcgtcgc ggctcagcgt ctgtccggcc ttcactgccg
     5641 ccgacttgca cactcgcttg atgacctgcg cctccagttg cgcccccagc ggcgcaacct
     5701 cgttctccag atcgctgacg acggccatga gcgccgtcgc cgggtcggtg cggcccaaca
     5761 gcgctggcac ggcccgcacc tggaacgcgc ccggcccaaa cggctcgatc tcgaagccca
     5821 gcccggccag caggccggcc tgctcgtcca ccagcgcggc ctgcgacggc ggcaggtaga
     5881 ccgtcgccgg agccaccagc ccctgcgacg ccacgcgccg ctgccgccac tgggccaata
     5941 gttgctcgaa aaggatgcgt tcgtgggcgg catgctggtc gattaggtac aacccctctg
     6001 gcccctcggc gatgatgtag gccgcgccga cctggccgat gacgcgcatg atgggcaggc
     6061 gcggctcgtg ggggatgagc ggcgggggcg cgactgctgg ctccacgccg gcgttgtccc
     6121 cgtcgccggg gttgtccccg tcgccggggt tgtccccgtc gccggggttg tccccgtcgc
     6181 cggggttgtc cccgtctagc gcatccagct cactctccgg cgcgggcggg cgcagttgcc
     6241 aggggatgtc gagttgcgcg gccggccggt cgccgtcgtc cttgcgcggg gcgaaggctg
     6301 gccggccgcc ccaactgctc cagccaccca gcgagtcgcc cgcaggccag ccgccggcgg
     6361 cgcggccggg cgtcgtgccc agcagcgcgg cgcgcacttc gcgctgcacg gcgctgaaca
     6421 tcgtcccctc gtcgcggaag cgcacctcga ccttggccgg gtggacattg acatcgacgt
     6481 cctccacggg cacatcgagg aagagcaggc ccagcggata gcggccaacg ggcagcaggg
     6541 tgtgataggc ctggacaacg gcgaaggtca gacggttgtc gcgcacggcc cggccattga
     6601 cgaacaactg gatgtggttg cggttggccc aattgagggc cgggtcgctg acgtagccgc
     6661 gcacgccgac gcggcgctcg tcgccctcgt ccgcggggat ggtgatgagc tggcgcaccg
     6721 tctccgggcc gtagacggcc agcagtacgc cggccgtctc gccgttgccc gtcgtctgga
     6781 aggtgatgcg gttgtcgtgg ctcaggcgaa agcggatgcc ggggtaggcc agcgcgtagc
     6841 gggtgacgaa ctcgtcgatg agccgcttct cggtggtgat gctcttcagg aacttcagac
     6901 gggcggggac gttgtagaac agattttcca cggcgatgac cgtgcccacc ggcgcgccag
     6961 cggtctcgcg cgtcatgatg tggccgccgt cgagcaccag gcgcgtgccc gccgtggcct
     7021 cgcgggcgcg gctgacgacg gtcacctggc taacggcggc gatggcggcc agggcctcgc
     7081 cgcgaaagcc gagagtgcgg atggcgtgca gatcgtcggc cgtgttcagc ttcgacgtgg
     7141 catggcgcag gaaagccgtc tcaacctcgc cggcgtcaat gccgctgcca ttgtcggcca
     7201 cctggatgag ctgccggccg ccgccgcgaa tctcgacggt gatggctgtc gccccggcat
     7261 cgatgctatt ctcaaccagc tccttgacga ccgacgacgg ccgctcgacc acctcgccgg
     7321 cggcgatctg cgacgccaat tggtcgggca aaacgtggat catggccgga ttttagcaga
     7381 tacgaccctc gaaacgtagg cctacgcggc gcttcaccag gggcataaca aagagctcac
     7441 gcaaaggcgc gaaggggcag aaggcgcaaa gaagaaacgg caggcgaata gaagattctt
     7501 ctttgcgcct tggccccttt gtgcctttgc gtgagcgctt cttccggctt tgtaataccc
     7561 caggacgtct cgccgcctac ggcgtttcga tgctgaccag ggcggtcatg ccccagcgca
     7621 gcggcaggtc gggggcgtcg tcgagggcga tgcggacgcg gtaggtcacg tcgccgag
//
LOCUS       DAOSMQ010000091         7637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9591_length_7637_cov_4.786336, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000091 DAOSMQ010000000
VERSION     DAOSMQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9591_length_7637_cov_4.786336"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1206
                     /locus_tag="PK829_04495"
     CDS             190..1206
                     /locus_tag="PK829_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU40506.1"
                     /translation="MDTDTSPPAATGGKTARQRLLTLLKLGVTALGLFLVLRGLDFGA
                     LLGALRTVRLSWMAIGAAGIALSLVVRAYRWHLVLHGVGSSIRFGRLVELYLVGSFFN
                     AFLPSGLGGDVVRAAEAAQDVAPGVAAGTVLVDRLTGLLALFGMALAVLPFRPDGFPP
                     RLAWTIGAICVGGLLGGLALIEGRLLRLVVDRLPGALRTAGDGFLDRLAATIHRCDWR
                     SVAAALLVSLVFNLMQIGWWAATGRALGLRVPFGYFVLVVPVMSLAVLVPSIGGLGVR
                     ETVAPALFAGAGLAPEQAVALTLLVFALERVASLLGAPVYLWTTARAARRLPRKEGQP
                     ARPR"
     gene            complement(1203..2396)
                     /locus_tag="PK829_04500"
     CDS             complement(1203..2396)
                     /locus_tag="PK829_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HOU40507.1"
                     /translation="MRIILYLGKGGVGKTTVAAATALRSAQLGYKTLVASTDIAHSLA
                     DSLDIPLGAMPIQVADNLWAQEISVVADIQNYWGTLQGFVSNVISGQGLNNVMADELS
                     AFPGMDEIVSLLHINKQAKEREFERVIIDAAPTGETIRLLTMPDTFRWYAGHVSKLES
                     AIVMRALKPFAGRLLQGPSEVLEAISNLDAATADLRKTLSDPSVSSYRVVLQPEKMVM
                     REAERAISYLGLFNYPVDSVVINRILPDVAEDSEFYRQRRAIQTKYIELIENNFRPLP
                     LWYAPYYAHEVVGLEALSQLASDCFGPTDPGEIFYRGILQEIIETEGGGYLLRLPLPF
                     VGRDDVRLRKRGDELFITIGNFKREMILPSVLAKRRAGGGRLRDGMLEITFSASEGEP
                     AAEAA"
     gene            complement(2406..4166)
                     /locus_tag="PK829_04505"
     CDS             complement(2406..4166)
                     /locus_tag="PK829_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU40508.1"
                     /translation="MNTIPLVYPTQPRQTVQARLPDGRVLEGPRGTPLEVFIEAADYP
                     AEEHVVAALMNNRLRELCFPLMEDADLKPVTTATNDGSRIYRRSLSFLMIAAAAEVLP
                     GQVITIHHSMPFGGYYCERGDAQRLTDAELVALRTRMRQLVDADLPINNIRVPLEEAL
                     AFFHESGDLEKADLFARRRKDYLTLYELNGVRDYFHGFMVPRTRYLKLFDLRHYNDGF
                     ILQFPRRTWANVLQPFEDEPRLAQVFQTYKDWLTIMGVGNVTSLNQAIVGGRSSEVIM
                     VAEALHERQLASIARAIASRQPSVRLVAVSGPTSAGKTTFAKRLALQVMAQGIYPVII
                     SLDDYFVDREATPLDPTGAYDFEALEALDLVLFQSDMCRLMAGEEVLLPRYNFKTGRR
                     EVGSPLSIGKNHVILIEGIHGLNPRLTEGLPESATFRVFISAFTQMNLDKHNRVPTTD
                     TRLLRRIVRDAAHRGYPAAATIGRWNSVRRGEKNHIFPYQNTADVFFNSALVYELSVL
                     KPLAEPLLLQVEPGTPERLEANRLMAFLQWFEPLPDADRVYIANDSILREFIGGSVLE
                     NFHPSHWYATGAISGRIALE"
     gene            complement(4238..5149)
                     /locus_tag="PK829_04510"
     CDS             complement(4238..5149)
                     /locus_tag="PK829_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU40509.1"
                     /translation="MPAALLALLAGALAFGHVSPARAATARPAPAVPLLSPFWGPDIQ
                     RWAGQIHALSLAYGFHPDFIAAVIEHEAEGEQRAVGHPRMAGLMSLLPARRGSQWQPS
                     AETMLGPASDLRWGMTVLSYVVQQAGGDLYTALAAYNGGWQAVGSRSASDYAAGVLDS
                     FARALLVRAGLSPQTASRWTVAIIIRAGNVSEESLLVLGHQPIAPLHIYAYHTVYAFA
                     DEAGHAYTVSGYVVPVGLSEGAVVESSAGPDELEAPLRARLGEKDAGSAMGNPRVLLA
                     CLAQLERLRGQVTTRWFAPLGCPAAER"
     gene            complement(5207..5731)
                     /locus_tag="PK829_04515"
     CDS             complement(5207..5731)
                     /locus_tag="PK829_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40510.1"
                     /translation="MKFIKQRLPGTLLVDGERAALRLHDDEAVVYLAFALVTQGIAAQ
                     IVPTVLLDDWGTEIKGLGLYDWVRENGMHFPRAEVFGFSPAGESIQYFLRDVELFAAY
                     PVYAAADKASPIADWRPVFSMLVPDSELSSPVAAAAPAALTGPLRQARVGWWRVPPRL
                     DALDFIEARDLPLP"
     gene            5990..6529
                     /locus_tag="PK829_04520"
     CDS             5990..6529
                     /locus_tag="PK829_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU40511.1"
                     /translation="MADFPTSGDSPDTDSDTLLATIEAARRLRHDDDLEGSQALLLGL
                     MEEFPDDPLVLFEVGGAYDVMGEVEMAIPYYQRAIDAGLEGSDRHECLLCLGSSLRLA
                     GETDDAVETLSQAIDEFPERNSGRVFLALAHYSNGDANEAVRDLLAVLLATTQDADIL
                     AYADTFDYVKDNLDETLDG"
     gene            6531..7601
                     /locus_tag="PK829_04525"
     CDS             6531..7601
                     /locus_tag="PK829_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOU40512.1"
                     /translation="MKALAFEEHGSIDRVQLLDVPIPAIGPREVLLSVRAAALNRLDL
                     WVLEGWPGLKLKLPHIMGADGAGVVAAVGEAVTRFQVGDRVAVNPTLCNDPADRFARL
                     GLDNMCDDFAIFGEHIDGLFAEYAAVPERNLLPLPDGVAFETAAAASLVFVTAWHSLV
                     TRGRLQPGESVLIVGAGGGVNTASIQIARLVGARPIYVVGSSAAKLAQARALGADEVI
                     NRAEADWGRELYRLTARQGVDAVVDNVGAATFAQSLRALRRGGRLLTVGNSSGPRFEL
                     DNRYLFAKHLTIIGSTMGTSVDYEKVMGLIFAGRLRPVIDTVYQLRDATTALTRLRDG
                     DVMGKLVLRIPPADVADKDQER"
BASE COUNT         1309 a         2499 c         2525 g         1304 t
ORIGIN      
        1 cactaccagg acggtttgct acccccctgt ttttgcgcaa aattagggtg tcaggttggc
       61 ggccacgaat ggtacttcca ccagaggacg actagtcgcc cacctatcag gtcaatggtc
      121 gccagacgac tttggcaaag ccctgactcc gtatgacgaa cgagagcgac tggactacaa
      181 tcgcgggcga tggacacaga cacctcaccg cccgccgcga ccgggggcaa aacggcccgg
      241 caacgactgc tgactttgct gaagctgggc gtcacagcgc tgggcttgtt cctcgttctg
      301 cgtgggctgg acttcggcgc gctgctgggg gcgctgcgca ccgtgcgact gagttggatg
      361 gccatcggcg cggccggcat cgccctgagc ctcgtcgtcc gcgcctaccg ctggcatctc
      421 gtcctgcatg gcgtcggctc gtctattcgc tttggccgcc tggtggaact ctacctggtg
      481 ggcagcttct tcaatgcctt tctgccgtcg ggcctgggcg gcgatgtcgt ccgcgccgcc
      541 gaggcggccc aggacgttgc gccgggcgtt gccgcgggca cggtacttgt cgaccggctg
      601 acggggctgc tggctctctt cggcatggcc ctggcggtgc tgccgttccg gccagacggc
      661 ttcccaccgc gcctggcctg gaccatcggc gcgatttgcg tcggcggtct gctggggggc
      721 ctggccctga tcgaggggcg gctgttgcgc ctcgtcgtcg atcggctgcc gggggcgctg
      781 cgaacggccg gagacggctt cctcgaccgc ctggcagcga ccatccaccg ctgcgactgg
      841 cgttccgtgg ccgcggcgct gttggtctcc ctcgtcttca acctgatgca aatcggctgg
      901 tgggcggcca ccgggcgagc gctgggcctg cgcgtgccct tcggctactt cgtgctggtt
      961 gtgccggtca tgtcgctggc ggtgctggtg ccgtccatcg gcggcctggg tgtacgtgaa
     1021 acggtcgcgc cagcgctgtt tgccggcgct ggcctggccc cggagcaggc cgtcgccctg
     1081 acgctgctgg tcttcgccct ggaaagggtc gccagcctgc ttggagcgcc ggtctacctg
     1141 tggacgaccg cgcgcgccgc gcgtcggctg ccccgcaaag agggacagcc cgcccgcccg
     1201 cgttaggccg cttcggccgc cggttccccc tcgctggccg agaaggtaat ctccaacatc
     1261 ccgtcgcgca agcgcccgcc accggcgcgc cgtttggcca gcacgctggg caggatcatc
     1321 tcgcgcttga agttgccgat ggtgatgaac agctcgtcgc cgcgcttgcg caggcgcacg
     1381 tcatcgcggc caacgaatgg cagcggcagg cgcagcaggt agcccccccc ttccgtctcg
     1441 atgatctctt gtaagatgcc gcggtagaag atctcgccgg ggtcggtcgg gccaaagcag
     1501 tccgaggcca gttgcgacag cgcctctaac ccgacgacct cgtgggcgta gtagggcgca
     1561 taccacaggg gcagcggccg gaagttgttc tcgatcaact cgatgtattt agtctgaatc
     1621 gcccggcgtt gccggtagaa ttcgctgtcc tcggccacgt ctggcaaaat gcggttgatg
     1681 acgacgctat cgacgggata gttgaacagg cccagatagc tgatggcccg ctcggcctcg
     1741 cgcatgacca tcttctccgg ctgcaagacg acgcggtagc tgctgaccga tgggtcgctc
     1801 agcgtcttcc gcagatcggc cgtggccgcg tcgaggttgc tgatcgcctc cagcacttcc
     1861 gatggcccct gcaacagccg cccggcaaaa ggcttcaggg cacgcataac gatggccgac
     1921 tctagcttgc tgacgtggcc cgcataccag cgaaaggtat cgggcatggt cagcaggcgg
     1981 atcgtctcgc ccgttggcgc ggcgtcgatg atgacgcgct cgaactcccg ctccttggcc
     2041 tgcttgttaa tatgtagcag gctgacgatc tcgtccatgc ccggaaaggc ggacaactcg
     2101 tcggccatga cgttgttcag gccctggcca ctgatcacgt tggacacaaa gccctggagc
     2161 gtaccccagt aattctggat gtcggcgacg acgctgatct cctgcgccca caggttatca
     2221 gccacctgga taggcatagc tcccagaggg atgtccagcg aatcggccag actatgagcg
     2281 atatcggtgc tggccacaag cgttttgtag cccagttgcg cgctgcgcaa cgcggtggcc
     2341 gcggcgacgg tggtcttgcc gacgccgccc ttccccaaat ataaaataat ccgcatgcta
     2401 ttctcctact ccaaggctat acgtcccgat atcgccccag ttgcatacca gtggctggga
     2461 tggaagttct ccagcaccga cccgccgatg aactcgcgca ggatcgagtc gttggcgatg
     2521 taaacgcggt cagcgtccgg cagtggctcg aaccattgca gaaaggccat cagccggttg
     2581 gcttccagcc gctccggcgt acccggctcg acctgcaaca acagtggctc ggccagcggc
     2641 ttcagcaccg acaactcata gaccagggcc gagttgaaaa aaacgtcggc cgtgttctgg
     2701 taggggaaga tgtggttctt ctcgccgcga cggacactgt tccaccggcc gatggttgcc
     2761 gcggcgggat agccccggtg ggcggcgtcg cggacaatgc gacgcagcag gcgagtgtcc
     2821 gtcgtcggca cacggttgtg cttatcgagg ttcatctgcg tgaacgcaga gatgaacacc
     2881 cgaaaggtag cggattccgg caatccttcg gtcaggcgcg ggttcaggcc atggatgccc
     2941 tcgatcagga tgacatggtt cttgccaatg ctaagtggcg aaccaacctc gcgacggccg
     3001 gttttaaagt tatagcgcgg cagcagcacc tcttccccgg ccatcagccg gcacatgtcc
     3061 gattggaaca ggaccaggtc gagcgcctcc aatgcctcga aatcataggc tccggttggg
     3121 tccagcggcg tggcctcgcg gtcgacgaag tagtcgtcga ggctaatgat aaccgggtaa
     3181 atgccctggg ccatgacttg caaggccagc cgcttggcga aggtcgtctt gccggccgaa
     3241 gttggcccgg agacggctac gaggcgcacc gacggctgac ggctggcaat ggcgcgggcg
     3301 atgctggcca gctgccgctc gtgcagcgcc tcagcgacca taatcacctc ggaagagcgt
     3361 ccgccgacga tggcctggtt gagcgaggtg acattgccca cgcccataat ggtcagccaa
     3421 tccttgtagg tttgaaagac ttgcgccagc cgcggttcat cctcgaaggg ctggaggacg
     3481 ttggcccagg tgcggcgtgg gaactgcaag atgaagccgt cgttgtaatg gcgcaggtca
     3541 aacagcttta gatagcgggt gcgcggcacc atgaagccgt ggaagtagtc gcgcacgccg
     3601 tttagctcgt agagcgtcag gtaatccttg cgccgccggg cgaacagatc ggctttctcc
     3661 aggtcgccgc tctcgtggaa gaaggccagc gcctcttcga ggggcacgcg gatgttgttg
     3721 atgggcagat cggcgtccac cagctggcgc atgcgcgtcc gcagggcaac caactcggca
     3781 tcggtcagcc gttgggcgtc accgcgctcg cagtagtagc cgccgaaggg catggagtgg
     3841 tggatggtga tgacctggcc gggcagcacc tcggccgcgg cggcgatcat caaaaagctt
     3901 aacgagcggc gatagatgcg cgagccatcg ttggtggccg tcgtcaccgg cttcaggtcg
     3961 gcgtcctcca tcagcgggaa gcacaactcg cgcagacggt tgttcatgag cgcggcgacg
     4021 acgtgctcct cggccgggta gtccgccgcc tcaataaata cttccagcgg cgtaccacgc
     4081 ggcccttcga ggacgcggcc atcgggtagc cgcgcctgga cggtctggcg gggttgagta
     4141 ggatagacaa gtgggatagt gttcatagca atacatttgg gcacaaatgg ttgtgccttc
     4201 agcgcaagcg tagcacggcc ggcgcgttgg ggcgaatcta ccgctcggcc gccgggcagc
     4261 cgagcggcgc aaaccagcgc gtcgtcacct ggccgcgcaa ccgttctaat tgcgccaggc
     4321 aggccagcag cacgcgcggg ttgcccatcg cgctgccggc gtccttctcg cccagtctgg
     4381 cccgcagggg ggcctccagt tcgtcggggc cggcgctgct ctcgaccaca gctccctccg
     4441 acaggcctac cggcaccacg taaccactaa ccgtgtaggc atggcccgcc tcgtcggcaa
     4501 aggcataaac ggtgtggtag gcatagatat gcaatggggc aatgggctga tgccccagca
     4561 ccaataatga ctcctcggag acgttgccgg cgcgaatgat gatggccaca gtccagcggg
     4621 atgccgtctg cggcgaaagc ccggcgcgca ccaacaacgc gcgggcgaag ctgtccagca
     4681 cgccggcggc gtagtcggat gccgagcgac tgccgacggc ctgccagccg ccgttgtagg
     4741 ccgccagggc cgtgtagaga tcaccgccgg cctgctggac gacataggac agcacggtca
     4801 tgccccagcg cagatcgctg gccgggccga gcatcgtctc agccgacggc tgccactgcg
     4861 acccgcgccg ggccggcagc aagctcatca ggccggccat gcgcgggtgc cccacggccc
     4921 gctgctcgcc ctccgcctca tgctcaatga ccgcggcgat gaaatcggga tgaaaaccat
     4981 aagccagcga gagcgcatgg atttggccgg cccagcgctg gatgtccggc ccccagaagg
     5041 gcgagagcag aggcacggct ggcgcgggcc gcgcggtcgc cgccctggcc ggggaaacat
     5101 ggccgaacgc cagcgccccg gccaacaggg ccagcagcgc ggctggcaac agccaccgcc
     5161 tcaggccagg cgtgcagtgg gaggggcggc gcggcgacat agagcctcag ggcaagggaa
     5221 ggtcacgcgc ctcgatgaag tccagagcat caaggcgcgg cggcacgcgc caccagccga
     5281 cacgcgcctg gcgcagcggc ccggtgaggg ccgcaggcgc ggccgcggcc acgggcgacg
     5341 acaattcgct gtcggggacg agcatcgaga atacgggccg ccagtcggcg ataggcgacg
     5401 ccttgtccgc ggcggcgtag acgggataag ccgcgaacag ttctacgtcg cgcaggaaat
     5461 actggatcga ctcgccggcc gggctgaaac caaacacctc ggcccgcggg aagtgcatgc
     5521 cgttctcgcg cacccagtca tacaggccta gccccttgat ctccgtgccc cagtcgtcga
     5581 gcaggacagt cgggacgatc tgcgcggcga tcccctgggt cacaagggca aaagccaggt
     5641 agaccacggc ctcgtcgtcg tgcaggcgca gcgctgcccg ctcgccatcg acgagcaagg
     5701 tgcccggcag ccgttgcttg atgaacttca cttgcttcgc tcctgtggcc ggccgcggcg
     5761 acagaacggc cggcacggcg cgccccttgt caggctgtcg tcgatctagt gtaccaacgc
     5821 gcggcgcgaa atccaatgtt tcgcttgccg gttgcgcggc cggggtctat cattcgacca
     5881 tatcccaccc atgccgcctg gagcacgggc cagggccagc gcgagcgctc gacggcgcgc
     5941 ttttgtccgg cgcctgcccg gcaactaccc ggcaagtacg aggctaacga tggctgactt
     6001 ccccacctct ggcgattccc ccgacaccga cagcgacaca ctcctggcaa cgattgaggc
     6061 ggcgcgccga ttgcggcacg acgatgacct ggaaggctca caggcattgc tgctgggcct
     6121 gatggaggag ttccccgacg atccgctggt gctgtttgag gttggcggcg cctatgatgt
     6181 catgggcgaa gtggagatgg ccatccccta ctatcagcgg gccatcgacg ccggactgga
     6241 gggcagcgac cgccacgaat gcctgctctg cctgggcagc agcctgcgcc tcgctggcga
     6301 gaccgacgat gctgtcgaaa ccctatcgca agcgatcgac gagttcccgg agcgcaacag
     6361 cggccgtgtc ttcctggccc tggcccacta tagcaacggt gacgccaacg aggccgttcg
     6421 cgacctgctg gccgtcctgc tggcgacgac ccaggacgcc gacatcctgg cctatgctga
     6481 caccttcgac tacgttaaag acaatctcga cgaaacgctg gacggctaac atgaaagcgc
     6541 tggcctttga agagcacggc agcatcgacc gcgtgcagct gctggacgta cccatcccgg
     6601 ctatcggccc acgcgaggtc ctgctgtccg tccgcgccgc ggctctcaat cgcctcgacc
     6661 tgtgggtgct ggagggctgg ccggggctga agctgaagct gccccacatc atgggcgctg
     6721 atggcgcggg cgtcgtcgct gccgtgggcg aagccgtaac gcgctttcag gtgggcgacc
     6781 gcgtagccgt taacccaact ctctgcaacg acccggctga ccgcttcgcc cgcctgggtc
     6841 tagacaatat gtgcgatgat ttcgcgatct tcggcgagca cattgacggc ctctttgccg
     6901 agtacgccgc tgtgccggag cgcaatctat tgcccctgcc cgacggcgtg gccttcgaga
     6961 cggccgcggc ggcgtcgctg gtcttcgtca cggcgtggca ctcgctggtg acgcgcggcc
     7021 gcctccagcc aggcgagtcc gttctgatcg tcggcgcggg tggcggcgtc aatacagcct
     7081 ccatccagat cgcccggctg gtgggggcgc gccccatcta cgtcgtcggc tcgtcggcgg
     7141 ccaagttggc ccaggcgcgc gccctggggg ccgacgaggt catcaaccgc gccgaggcag
     7201 actgggggcg tgaactgtat cgcctgacag cgcggcaggg agtcgatgcc gtcgtcgata
     7261 acgtcggcgc ggcgaccttt gcccagtcgc tgcgggcctt gcggcgcggc ggccggctgc
     7321 tgaccgtcgg caactccagc ggcccgcgct ttgaactcga caatcgctac ctctttgcca
     7381 agcacctgac catcatcggc agcacgatgg gcacaagcgt ggactatgag aaggtcatgg
     7441 ggcttatttt cgccgggcgg ctgcggccgg tcatcgacac cgtctaccag ctacgcgacg
     7501 ccacgaccgc cctgacccgc ctgcgcgacg gtgacgtgat gggcaagctc gtgctgcgca
     7561 ttccgccagc cgacgtcgcg gacaaggacc aggaacgatg acgcccccca gcgccgaaac
     7621 caggaagtgg gcatgac
//
LOCUS       DAOSMQ010000092         7597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9661_length_7597_cov_4.107664, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000092 DAOSMQ010000000
VERSION     DAOSMQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9661_length_7597_cov_4.107664"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PK829_04530"
     CDS             complement(<1..371)
                     /locus_tag="PK829_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOU40513.1"
                     /translation="MTQVKICGLTRYEDALAALEAGADYLGFIFYPPSPRAIAPEAAA
                     ALVARLRADAAAHFARPERPRLVGVFVNESPAAVAALLARCGLDLAQLSGDEPPEAVA
                     DPTSPLFGRAYKALRPRSATEA"
     gene            complement(358..2154)
                     /locus_tag="PK829_04535"
     CDS             complement(358..2154)
                     /locus_tag="PK829_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU40514.1"
                     /translation="MAEPLVQLALVAVVGVGAQLAAWHLRLPSILLLLLSGFLLGPVF
                     GLIHPDALLGEALFPFVSLAVGLILFEGGLTLKLKELPTSGPAVSRLISVGALATWLV
                     AALGARLLLGFDWSLAIVLGAILIVTGPTVVGPLLRQIRPKGRSGAILKWEGILIDPV
                     GAVLALLVVEIITLGGGINVPGFVLLGVGRSLIVGVVLGILGAAVLVALMRRHLIPDY
                     LQSGITLVLVLAFFTLGDLLAAEGGLLTVTLMGIALANQTWVPMSHIVEFKENLQVLL
                     IGVLFILLAGRVQIADLLAAGWPALAFVALLILVARPLGVLLSTWRTPLSNAERLFLM
                     CMAPRGIVAASVASVFVFELSAAGVAGAELLSPVVFLVIVGTVIFYGLTAGPVARRLG
                     LAEREPQGALIIGAHPFARQLARALTDQNVRAVLVDTNYHHVTESRLAGLEAYHGNAL
                     AEETSDDLDLGGLGRVLALTGNDEVNTLTMLHFSDVFSRAEVYHLPATSARQPNGLRA
                     GRTLFGPGAHYDALANALDNGATLRATPLSNTFTYADYKAHYGQRATPLCMLTENRRV
                     QFFTSDNRPTPKPGHTLISLVAADGESGDDPG"
     gene            complement(2172..3086)
                     /locus_tag="PK829_04540"
     CDS             complement(2172..3086)
                     /locus_tag="PK829_04540"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40515.1"
                     /translation="MNLYVARHSDAAGFLAAAESWLLAREAHNSLILGVALAQRRQAQ
                     TRRRRLPIYLTVSDATGLLLAAVSTAGIRLLLAGDDTTDDDALDALVEQLPRSMPEVF
                     AEQKLARRFAARWSAARWPATTGPPATIGERQHLLQVTGVTLTAVTLLPHLPAGRLRP
                     ATVREAELLGDWLFAFQTTITSDDTVDREAASMIVNGLLARKDIYVWAVGEGAPERPV
                     SMAIRSRPTANGTAISYVYTPAAERRHGYATACVVHLSQSLLDDGWQFCTLFVEDGHT
                     TARNIYHRLGYQPIAEFAGYRLMPAPAP"
     gene            complement(3118..3744)
                     /locus_tag="PK829_04545"
     CDS             complement(3118..3744)
                     /locus_tag="PK829_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOU40516.1"
                     /translation="MKLIIPSFENGAPIPAEFAMGIPAPDAPATFGPNRSPHIVWADA
                     PAGTQSFTLICTDHDAPSVRDDANQPDRALPYDLPRVDFYHWVLVDIPADVRELPAGC
                     DCEGVTPRGKEVGPTPYGRRGINSYTSWFAGETSMEGDYGGYDGPFPPWNDERVHHYH
                     FVLYALDVPTLGLSGAFDGADALLAMRGHILAEADWVGTYAIYPQARL"
     gene            complement(3767..4669)
                     /locus_tag="PK829_04550"
     CDS             complement(3767..4669)
                     /locus_tag="PK829_04550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU40517.1"
                     /translation="MNHMQGQVCLVTGANAGIGKQTARELAQMGATVVLVARNRERGE
                     AARAEIAASGASVELLLADFSSPASIRAMTEAFKARHDRLDVLVNNAGVYLMKREETA
                     DGLEMTFAVNHLGYFMTTLLLWDRLLAARSARVIVLASDAHRQAKLNFDDLQNRRKYA
                     GFRVYAQSKLANVLFTYELDRRRGNAPITINAVHPGFVASNFGRNNGGVAGLFMTRIV
                     PLFARTVVEGAATSVYLASSPEVAGISGQYFTDSRAVKSAPQSYDRATAERLWAISEE
                     LTGLRLPNLALPDERRENGHQTDL"
     gene            complement(4720..5622)
                     /gene="aroF"
                     /locus_tag="PK829_04555"
     CDS             complement(4720..5622)
                     /gene="aroF"
                     /locus_tag="PK829_04555"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOU40518.1"
                     /translation="MAAFIAESSPNGVTEMMAQERILEMEQADMALVARPVTVAPDVV
                     IGGRDVVVMAGPCSVETYEQTRAVAAAVWAAGGRVLRGGAFKPRTSPYSFQGLGREGL
                     EILRAVADEFGLLVISEVLGVENLPLVAEYADILQIGSRNMQHYPLLWAVGASDRPVL
                     LKRGFMSTIDEWLKAADHILSRGNNRIILCERGIRTFETATRNTLDTNAIALARQIAP
                     YPVLADPSHATGRADLVLPAARAAVAAGADGLLVEFHPDPAAALSDGEQALPLEALPR
                     FYDEVERVARALGRGLSRGPLASQ"
     gene            5621..6556
                     /locus_tag="PK829_04560"
     CDS             5621..6556
                     /locus_tag="PK829_04560"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOU40519.1"
                     /translation="MTTAVDSSRRFAVWLNAARPRTLPLALACIITGTGLAAAEGAFN
                     WPVALTCVVTAILLQILSNLANDYGDSLHGADHVERRGPKRAVQSGLVTPAEMRRAIV
                     ITALATMAAGLLLLWLAFGREAFSSTLLFIVLGGAAIGAAITYTAGRLPYGYAGLGDL
                     AVLIFFGWVGVLGSYFVQAQSFAAVLLLPATACGLLAVAVLNVNNIRDLESDRLAGKN
                     SIPVRLGLRRARGYHWLLLALAVVSATAYVLLRYTSLWQFLFLLAVPLLVRNGLAISR
                     TADLATLNPWLKQMSLAALVFALLFTLGQVLPLWL"
     gene            complement(6566..7366)
                     /gene="menH"
                     /locus_tag="PK829_04565"
                     /pseudo
     CDS             complement(6566..7366)
                     /gene="menH"
                     /locus_tag="PK829_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
     gene            complement(7363..>7597)
                     /locus_tag="PK829_04570"
     CDS             complement(7363..>7597)
                     /locus_tag="PK829_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40520.1"
                     /translation="VGLPHPEWGQQVAAAVVLRRPGAATAGALLAHCRARLPGYKCPR
                     RLRLVDALPQTASGKIQRRLVAETWPAEEQQAA"
BASE COUNT         1253 a         2461 c         2675 g         1208 t
ORIGIN      
        1 gcctcagtcg ccgagcgggg acgcagggct ttgtaggctc ggccgaacag aggcgaggta
       61 gggtcggcca cagcctccgg cggttcgtcg ccgctcaact gcgccaggtc gagaccgcac
      121 ctggccagca gcgcggcgac ggcggcgggc gactcgttga cgaagacgcc gaccaggcgc
      181 ggccgctcgg gtcgggcgaa gtgagcggct gcgtcggcgc gcagccgggc caccagcgcg
      241 gcggcggcct ccggagcgat ggcccgcggg ctgggtgggt agaagatgaa gcccagatag
      301 tccgcgcctg cctccagagc cgccagcgcg tcttcgtagc gagtcaggcc gcagatctta
      361 acctgggtca tcgccgcttt ctccgtcggc cgcgaccagg ctgatgagcg tgtggcccgg
      421 tttaggcgtt ggccgattgt cgctcgtgaa gaactgtacg cgccggttct ccgtcaacat
      481 gcagagaggt gtagcccgtt ggccataatg ggctttgtag tcggcatagg tgaacgtgtt
      541 gctgagaggt gtggcccgga gcgtcgcccc gttgtcaagg gcgttggcca gagcgtcata
      601 gtgtgcgccg gggccgaata gcgtgcgccc ggcgcgaagg ccgttgggtt ggcgggcgct
      661 tgtggccggc aggtggtaaa cctcggcccg gctgaagaca tcgctgaagt ggagcatggt
      721 cagtgtgttg acctcgtcat tgcccgtcag ggccaggacg cgccccaggc cgcccaggtc
      781 gagatcgtcg ctcgtctcct cggccagggc gttgccgtgg taggcctcca gccccgccag
      841 gcggctctcg gtgacgtgat ggtagttggt atcgacgagg acggcgcgga cgttctgatc
      901 agtcagggcg cgggccagct gccgggcgaa aggatgcgcg ccgatgatca gcgcgccctg
      961 gggttcgcgc tcggccagcc ccagccggcg ggcaactggc ccggccgtca gaccgtagaa
     1021 gatgaccgtg ccgacaatga ccaggaagac gaccggcgat agtagctccg ctccggccac
     1081 gcccgccgcg gacagctcaa agacgaacac agacgccacc gacgcggcga cgatgccacg
     1141 cggggccatg cacatcagga agaggcgttc ggcgttgctg agaggcgtgc gccaggtaga
     1201 cagcagcacc cccagcggac gggccaccag aatgagcagg gccacaaagg ccagcgccgg
     1261 ccaaccggct gccagtaggt cagcgatctg cacgcgcccg gccagcagga taaacaggac
     1321 gccgatcagc aggacttgca ggttctcctt gaactcgaca atgtggctca tgggaaccca
     1381 cgtctggttg gccagggcga tgcccatgag cgtgaccgtc aacaggccgc cctcagccgc
     1441 cagcagatcg cccagcgtaa agaaggccag caccagcacc aacgtgatgc cgctttgcaa
     1501 gtagtcaggg atgaggtggc ggcgcatgag ggccaccagg acggccgcgc cgaggatgcc
     1561 caggacgacg ccaacgatga ggctgcggcc gacgcccagc aagacgaagc cgggcacgtt
     1621 gatgccgccg ccgagggtga tgatctcgac gacgagcagg gccagcaccg cgccgacggg
     1681 gtcgatgaga atcccttccc atttcaagat cgccccgcta cgccctttgg ggcgaatctg
     1741 gcgcagcagc gggccgacga cggtcgggcc ggtgacgatc agaatcgcgc cgaggacaat
     1801 ggccaacgac cagtcgaagc cgagcaagag gcgcgccccc agcgcggcca ccagccaggt
     1861 cgccagcgcg cccacagaga tgagccgcga gacggccggg ccgctggtgg gcagttcttt
     1921 cagcttcagc gtcaggccgc cctcgaagag gatcaggccg acagccagcg aaacgaacgg
     1981 gaagagagcc tctcccagca gagcgtcagg atgaatcaga ccaaagacgg gaccgagcaa
     2041 gaaaccggac agcagcaaga gcaggatgga tggcaggcgt aagtgccagg cggccagttg
     2101 cgcgcccacg ccgacgacgg cgacgagtgc cagttggaca agtggttcag ccacgatgat
     2161 gttcctaagg gttatggggc tggggccggc atgagccggt agccggcgaa ttcggcaatc
     2221 ggctgatagc cgaggcggtg gtagatgttg cgcgccgtcg tatgaccgtc ttcgacgaac
     2281 aaggtgcaga actgccagcc gtcatcgagg agcgactggc tgagatggac gacgcaggcg
     2341 gtcgcgtagc cgtggcggcg ctccgctgcc ggtgtgtaga cgtagctgat ggccgtgccg
     2401 ttcgccgttg gacgcgaccg gatggccatc gacaccggcc gttctggcgc tccctcgcct
     2461 acggcccaaa cgtagatatc tttgcgggcc agcaacccgt tgacaatcat gctggctgcc
     2521 tcgcgatcga ccgtgtcgtc ggacgtgatg gtcgtctgga aggcaaagag ccagtcgccg
     2581 agcaattcgg cctcgcgcac agtggccggc cgcaaccgcc cggcgggcaa gtggggcaac
     2641 agggtcacag ccgtgagggt cacgccggtg acctggagca gatgttggcg ctcgccaatt
     2701 gtcgccggcg gcccggtcgt cgccggccac cgcgcagccg accaccgcgc cgcgaaccgg
     2761 cgcgccagct tttgttcggc gaagacttcc ggcatggagc gcggcagctg ctcgacaagc
     2821 gcgtcgagcg cgtcgtcgtc cgtggtgtcg tcgccggcca atagcagcct gatgccggcc
     2881 gtggaaaccg ccgccagaag caggccggtg gcatcgctaa ccgtcagata gatgggcagc
     2941 cggcggcggc gcgtttgggc ctggcgacgc tgggcgaggg ccacgccgag gatgaggctg
     3001 ttgtgcgctt cgcgcgcgag cagccaggac tcagccgccg ccaaaaatcc ggccgcgtcg
     3061 ctgtgtcggg cgacgtagag attcatgacc cgtagttcac agcccacgcg gcaggagcta
     3121 tagccgtgcc tggggataga tggcgtaggt gccgacccaa tccgcctcgg ccaggatgtg
     3181 gccgcgcatc gccagcagcg cgtcggcccc gtcgaacgcg ccagaaaggc ccagcgtggg
     3241 cacgtcgagg gcatagagaa cgaagtggta atggtggacg cgctcgtcgt tccagggagg
     3301 gaaggggccg tcgtagccgc cgtagtcgcc ctccatgctc gtttcgccgg caaaccagct
     3361 ggtgtagctg ttgatgccgc gccggccgta gggcgtcggg ccgacttcct tgccgcgcgg
     3421 ggtgacgccc tcgcagtcgc agccggccgg cagctcgcgc acatcggcgg ggatgtccac
     3481 cagcacccag tggtagaagt cgacgcgggg caggtcgtag ggcagggcgc ggtcgggttg
     3541 gttcgcgtcg tcgcgcaccg aaggcgcgtc gtggtcagtg cagatcaggg tgaacgactg
     3601 cgtgcccgcc ggcgcgtcgg cccagacgat atgggggctg cggttggggc cgaaagtggc
     3661 cggcgcgtcg ggcgcgggga tgcccatggc gaactcggcc gggatgggcg cgccattctc
     3721 gaacgacggg atgatcagct tcatgggtta cctccttgcc ggttacttaa aggtcagtct
     3781 ggtggccatt ttcccgcctt tcatcaggaa gggcaaggtt gggcaggcgg aggccggtca
     3841 gctcttcgct gatcgcccac agccgctcgg ccgtggcgcg gtcgtagctc tggggggcag
     3901 acttgacagc gcggctgtcg gtgaagtact gcccgctgat gcccgccacc tcgggcgacg
     3961 acgccagata gaccgaagtc gccgcgccct cgaccaccgt ccgggcgaac aggggcacaa
     4021 tgcgcgtcat gaacaggccg gcgacgccgc cgttgttgcg gccaaagttg ctggcgacga
     4081 agccgggatg gacagcattg atggtgatgg gcgcgttgcc acggcggcgg tcgagttcgt
     4141 aggtgaagag cacgttggcc agcttcgatt gcgcgtagac gcgaaagccg gcgtacttgc
     4201 gccggttctg caggtcatcg aagttgagct tggcctgccg gtgggcatcg gaggccagaa
     4261 cgatgacacg ggccgaccgg gccgccagca ggcgatccca cagtagcagc gtcgtcatga
     4321 agtagcccag gtggttgacg gcgaaggtca tctccagccc atcggctgtc tcctcgcgct
     4381 tcatcaggta gacgccagcg ttgttgacca gtacatccag ccggtcgtgg cgcgccttga
     4441 acgcctcggt catggcccga atggaagccg gcgaggagaa gtcagccagc agtagctcga
     4501 ccgaggcccc gctcgcggca atctcggccc gcgccgcttc accccgttcc cggttgcggg
     4561 cgaccaggac gacggtggcc cccatttgcg ccagttcgcg cgccgtttgc ttgccaatgc
     4621 cggcgttcgc gccggtgacc aggcagactt gcccttgcat gtgattcatt tttcagtgtt
     4681 taccttgtcg tgtcgtggtg gtttggtggg cgcccgcggc tattgcgagg cgaggggacc
     4741 ccggctcaac ccccgcccca gcgcccgggc gacacgctcg acttcgtcgt agaagcgcgg
     4801 caaagcctcc agtggcaagg cttgctcgcc gtcagagagg gccgcggccg gatcgggatg
     4861 gaactcgacc agcagaccat cggctccggc ggcaacggcg gcgcgggcgg ccggcagcac
     4921 caggtcggcc cggccagtgg cgtggctggg atcggcgaga acggggtagg gggcgatctg
     4981 gcgagccagg gcgatggcgt tcgtgtccag tgtgttgcgt gtcgccgtct caaaggtgcg
     5041 gatgccgcgc tcgcacagga tgatgcgatt gttgccgcgg ctgaggatgt ggtcggcagc
     5101 cttgagccac tcgtcgatgg ttgacataaa gccgcgcttg agcaacaccg gccgatcgct
     5161 cgcgcccacg gcccacagta gcgggtagtg ctgcatgttg cggctgccga tttgcaggat
     5221 gtcggcatac tcggccacca ggggcaggtt ttcgacgccc agcacttcgc tgatgaccag
     5281 cagcccaaac tcatcggcca cagcgcggag gatttccaac ccctcgcggc ccaggccctg
     5341 gaagctgtag ggcgaggtgc gcggcttgaa cgccccgccg cgcagcacgc gcccaccagc
     5401 ggcccagaca gccgcggcca cagcccgcgt ctgctcatag gtctccacag agcacgggcc
     5461 ggccataacc accacgtccc gcccgccgat gacgacatcg ggcgcgaccg tcaccggccg
     5521 ggccacgagt gccatgtctg cctgttccat ttccagaatt cgttcttgtg ccatcatttc
     5581 cgttaccccg ttggggctac tctctgctat aaacgcagcc atgaccactg ctgtcgattc
     5641 atcgcgtcgc tttgctgtct ggctcaatgc cgcccggccg cgtaccctgc cgctggcgct
     5701 ggcctgcatc atcaccggta cgggtctggc cgcggctgag ggcgcgttca actggcctgt
     5761 cgccctgacg tgtgttgtca cggccatcct gctgcaaatc ctgtccaatc tggccaacga
     5821 ctatggcgac tcgctccacg gcgccgacca cgttgagcgc cgtgggccaa agcgggccgt
     5881 ccagagcggc ctggtgacgc cggcggagat gcgccgggcc atcgtcatca cggctctggc
     5941 gacaatggcc gcgggtttgc tgctgctgtg gttggccttc ggccgcgagg cgttctcgtc
     6001 cacgttgctg ttcattgtgc tcggtggcgc ggccatcggc gcggccatca cttacaccgc
     6061 cggccggctg ccctatggct acgccggatt gggcgatctg gcggtgctga tcttcttcgg
     6121 ctgggttggc gtgctgggca gctacttcgt gcaggcgcag agcttcgctg ccgtgttgct
     6181 gctgccagcg acggcctgcg gcctgctggc tgtcgccgtg ctcaacgtca acaacattcg
     6241 tgatctggag tccgaccggc tggcaggcaa gaactctatt ccggtgcgtc tgggcttgcg
     6301 gcgggcgcgt ggctaccatt ggctactcct ggccctcgcc gtcgtcagcg ccacggccta
     6361 cgtgttgctc cgctacacct cgctctggca gttcctcttc ctgctggccg tgcccttgct
     6421 ggtgcgcaat ggcctggcca tcagccgcac cgcagacctg gccacgctca acccctggct
     6481 taaacagatg tcgctggccg cgctggtctt cgccctcctg ttcaccctcg gtcaggtgtt
     6541 gccactctgg ttgtaggctc ctgtccagaa agtccaggat cgttgccaga aacgcctccg
     6601 gctgctccag atgaaccgtg tgaccagcat ggggcacaag gcgcaagtcg gccgccggca
     6661 accccgtggc catgcgccgg ttgatgttca cgaacttgtc atcctcggcc ccgacgacca
     6721 gcagcgttgg ggcaaccaac tccggcaatc gcgcccacag cgacggctgc gcgcccgtgc
     6781 ccatgccacg caagctgttg gccaaaccgg tggcgctgtt ggccagccgt tggcggcgta
     6841 gttgcgcctg ggcctcagcc ggcagccggg ataggccggc gaagaggggc agttgttccc
     6901 actcgtccac aaagcgggcc atgccatcgc gctcgatgcg ggccgcgagg gcctcatcgg
     6961 cgacgcggcg ctgctcgcgc ccggcagccg tcgccaggcc aggcgaagcg ctctccagaa
     7021 tgagcgagcg gacggcgtgc gggtggtgga gcgcggcgta gagggccagg cggccgccca
     7081 tcgaatagcc cagccagtgc gcgggcaggg cggcacggca ctccaaaagc gctacgagat
     7141 cagcggcgac gtaatttatc gtgtagccag cgggatcgac cggggcgtcg ctgccgccgt
     7201 ggccgggcag gtctacggcg atgacgcgga agcgctcggc cagccttggc cagaggccgc
     7261 gccaggtggc cgcgctgccg gtgaagccgt gcagaagcag gagcggctcg cccgcgcccg
     7321 cctcgtcaat ggcgtaacgc aggccgttga cggtttcgta gctcatgccg cctgctgctc
     7381 ctcggccggc cacgtctcgg ccaccagacg gcgctgaatc ttgccagagg cggtctgcgg
     7441 cagcgcgtcg accagccgca ggcggcgcgg gcatttgtag ccgggcaggc gcgcccggca
     7501 atgggccagc agcgcgccgg ccgtggccgc gccgggccgg cgcaggacga cggcggcggc
     7561 gacctgttgg ccccactcgg ggtggggcag accaacg
//
LOCUS       DAOSMQ010000093         7573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9697_length_7573_cov_4.669460, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000093 DAOSMQ010000000
VERSION     DAOSMQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9697_length_7573_cov_4.669460"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2763)
                     /locus_tag="PK829_04575"
     CDS             complement(<1..2763)
                     /locus_tag="PK829_04575"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU40521.1"
                     /translation="MHPKLPLFPMSANTPRRVGFVRPLAALLLLAMLATLWPRAAGAA
                     EQPAATATFSLGALNGPNGFRIDGRAAGSEAGRALRSAGDVNGDGLNDIIIGAAQAAP
                     DGMQRAGEAYVVFGTTTNPTSLNLTSLDGKNGFRLTGALFNDATGTAVAGGDVNGDGL
                     ADLLVGAPGAGVGAADGVGVVYVVYGAASFPARLNLGALDGSRGFRIEGSAAGDETGG
                     AVGYAGDQNGDGLGDILIGAPGATVGGKEAAGRAYVIFGRRNFPPRLSLPGLGSAGVA
                     ISGVSADSGVGHVVGAAGDMNGDGYDDSLIAAPGASRAGANEAGAVYVLFGQATLPTS
                     LTVSQISGTTGFRAEGAAAGDHAGGAADGGGDVNGDGRDDLLIGAPDAAGYDGRAYVV
                     LGANALPNVVGLGALSGASGVRLLGSEAHGASGAAVGLADVDGDGYDDILVGAAAAGT
                     GSARFAGRVDILFGRPTLAAEINLGALDANSGLSVLGAVAGDQAGQALSGGGDWDGDG
                     FDELLVGAPNRGIGPSGNQGAVFLVMGGPTLGIPNRVTHPGTPADNTLIGTSAADVLH
                     GHRGDDRLDAADGNDALKGGQGDDVLLGGAGGDALIGGAGLDVASYAASPASVTVNLF
                     TGAAGGGHAQGDRLRSIESLVGSPQADALTGDAGDNRLDGGPGDDSLTGGLGDDRFAF
                     AANSGNDTIAGFVPGAGSVDSLDFTGLPGVGGVGDLTIQADGGNTRITLPAGATIRLL
                     GVAPAALHADDYRFAGAPLAHPDAYSTPVNAPLAVAAPGVLANDENPSAVALTAVLVA
                     GPSHGTVNLKANGSFTYAPAANFVGEDTFTYRANNGQNSNTAAVTIVVTPRRPTAVND
                     SYSVALGETLTVAAPGVLANDSNPGNAPLTAVLVEEPLSGTLSLAANGSFIYTSTEEF
                     AGQDSFT"
     gene            2952..4715
                     /locus_tag="PK829_04580"
     CDS             2952..4715
                     /locus_tag="PK829_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40522.1"
                     /translation="MSEFAVELRRESNRRSPGRWVLSHFSRHGGLFLIMFIGAFGNAA
                     LAAVMPIFIGIAFNAALADPPDFQRIAIAAIVILASQIARSVLQFGRNFASSVIAERI
                     ERDSRQELYLSLLGKSMTFHDLQPVGDTMARATNDVHELNLMMMPGVNLVVGSANFLI
                     MPLILAPRYDMQLIAAPLFFIVTYVLALARYMNILSRIADRVRRAFGVMNARLAEALD
                     GVEMVKGSAQESGEVARFEANAREYRHATVAQGREEAKFLPLLLLGVTQAIGFTHALL
                     LLQAGRLQVGDVVAYVGLLSLFGFPTNISLMAYSRVSLGLASARRILDLVNRETQLDE
                     NPEGYAEPIVGDITFEGVSFGYVPNVDVLRDVRFTVRAGQTVAIVGQTGAGKSTLTKL
                     INRTYDAREGRVLVDGVDVRDWSLAALRRQISIIEQDIFLFSRTIAENIAFGAPEATA
                     EEIEAAARAAQAHDFIVGFQDGYQTVVGGRGVTLSGGQRQRIALARAFLTQPRILILD
                     DSTSAVDSATEDQIQRAIQHAAQNRTTFLITHRLSQIRWADLVLVMRQGRLEAAGDHA
                     TLMAVSPAYRRIFDQYEALEG"
     gene            4721..5029
                     /locus_tag="PK829_04585"
     CDS             4721..5029
                     /locus_tag="PK829_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40523.1"
                     /translation="MGPLVYYCRWRGVQLRLRGRDESAVWGQLVERRREDGRERAEPF
                     RFDLRTWELWLGEPPGRRLQLDELGVVESEEPSPDVSSSADDWPRTRWEDSSDVSTDS
                     "
     gene            complement(4959..7175)
                     /locus_tag="PK829_04590"
     CDS             complement(4959..7175)
                     /locus_tag="PK829_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOU40524.1"
                     /translation="MSLADKAHGDRARPLGDTYLDHARAVAAGLGELGIDETDAIAAA
                     LLHDILLPHTGISEAMLRAQFGETVADLVKSVNTLDDYAQRAGRSRPADELNLDATQD
                     RRTLEVVRRALLSIIEGDIRIILIRMVDCLQDLRRAASIDRDGQRRIAFEALHVYAPI
                     ANRLGIWHLKWQLEDLAFRYLEPEKYREIARRLDDGRDIRAVKINKAAARLRRRLDSL
                     GLKANVTGRPKHTYSIYRKMARKELSFDQIHDVQALRVILEPPDKDAYAALSSKARAD
                     MDQALCYQALGAVHSLWQPIRGEFDDYIGSPKANGYMSLHTAIIDAETGQKLEVQIRT
                     QRMHEEAEHGIAAHWAYKEQGAKVTSSGQKRIQNLRELLATLQEVEDDPEGTPLPDIS
                     RLEERIHVFTPKDDVIDLPAGATPIDFAYQIHTELGHRCRGARVNGKPVSLSYRLKSG
                     DRVDIIAPKPDSLKRSAPNRDWMNPSLGYTGSARTRSKIRHWFRQQEREQNIQQGREV
                     VQRELRQLFLHDVYTVEDIAGALKLGSVEDCLSRIGFGDIHSKRIIGAITALQYESRR
                     KQEQEALSADEHADLRPLLQPPSDKPKSGLTVRGVGGLYTRLAHCCSPVAPEPIVGYI
                     TRGQGVTIHARDCKQVAAITDRERLIEVEWGVEAETHPVSIVVKAVQRSGLIEDIINL
                     LRGEQVNVPKTKMITKDTVTTLYMIAEVKDLTQLSYLLTRLKNLPNVFEASRQHWN"
BASE COUNT         1116 a         2513 c         2609 g         1335 t
ORIGIN      
        1 ggtgaagctg tcctgcccgg caaactcctc ggtagacgtg taaatgaagc tcccattggc
       61 cgccaggctc agcgtgccgc tcagcggttc ctcgaccagc acagccgtca gaggcgcgtt
      121 gccgggattg ctgtcgttgg ccaatacgcc cggtgcggcg acggtgagcg tctcgcccag
      181 ggcgaccgag tagctgtcgt tcacggccgt cggccgccgt ggtgtgacga caatggtcac
      241 agcggcggtg ttggagttct ggccgttgtt ggcccgatag gtgaaagtgt cctcgccgac
      301 gaagttggcc gccggcgcgt aggtgaagct gccgttggct ttcaggttaa ccgtgccgtg
      361 cgacggcccg gcgacgagga cggccgtcag cgccacggcc gaggggttct cgtcgttggc
      421 cagaactccc ggcgcagcta cggccagggg cgcgttgacc ggcgtgctgt aggcatccgg
      481 gtgggccagc ggcgcgccgg caaagcggta atcatcggcg tgcagcgcgg ccggagcgac
      541 acccagtagc cggatcgttg ccccggcagg cagggtgata cgcgtgttgc cgccgtcggc
      601 ctggatggtc aggtcgccta cgccgccaac gcccggcaga ccggtgaagt cgagcgaatc
      661 gacgctgccc gcgccgggca cgaagccggc tatcgtgtcg ttgccgctgt tggcggcgaa
      721 ggcgaaacgg tcgtcgccca ggccgccggt caggctgtcg tcgccggggc cgccgtccag
      781 gcggttgtcg ccggcgtcgc cggtcagcgc gtcggcctgt ggcgagccga ccagactctc
      841 gattgagcgc aggcgatcgc cctgggcatg accgccgccc gccgcgccgg tgaagagatt
      901 gacggtgacg ctggccggcg acgcggcata cgaggccacg tctagacccg cgccgccgat
      961 gagggcgtcg ccgccggccc cgcccagcaa cacgtcgtcc ccctggccgc ccttcagcgc
     1021 atcgttgcca tcggcggcat caagacggtc gtcgccgcgg tgtccgtgca gcacgtcggc
     1081 cgcgctcgtg ccgatcagcg tgttgtcggc cggcgtgccg gggtgggtga cgcggttggg
     1141 gatgcccagc gtcggcccgc ccatcaccag gaacacggct ccctggttgc ccgatgggcc
     1201 gatgccgcga ttgggcgcgc cgacgagtaa ctcgtcgaag ccatcgccgt cccagtcgcc
     1261 gccgccgcta agcgcctggc cggcctggtc gccggcgacc gcgcccagga cgctcagacc
     1321 gctgttggcg tcgagcgcgc ccaggttgat ctcggctgca agcgtgggcc ggccgaacaa
     1381 gatatcgacc cgcccggcaa agcgcgcgct gcccgtgccc gccgccgccg cgccgacgag
     1441 aatatcatcg taaccatcgc cgtccacgtc ggccagcccg acagccgcgc ccgacgcgcc
     1501 gtgggcttcg ctacccagca ggcggacgcc actcgcgccg ctcagcgctc ccaggccgac
     1561 gacgttgggc agcgcgttgg cccccaggac aacgtaggcg cgaccgtcgt agccggctgc
     1621 gtcgggcgcg ccgatgagca ggtcatcgcg gccgtcgcca ttgacatcgc cgccgccgtc
     1681 ggccgccccg ccggcgtggt cgcccgcggc cgcgccctcg gcgcgaaagc cggtcgtgcc
     1741 gctaatctgg ctgacggtca ggcttgtggg cagcgtggcc tggccgaaga gtacgtagac
     1801 cgcgccggct tcgttcgccc cggcccgcga cgcgccgggg gcggcaatca ggctgtcgtc
     1861 atagccgtcg ccgttcatgt cgccggccgc gccgacgaca tggccaacac cgctatcggc
     1921 gctgacgccg ctgatggcca cgccggcgct gcccagaccc ggcagcgaca ggcgtggcgg
     1981 gaagttgcgc cggccgaaga tgacgtaggc ccgaccggcg gcctctttgc cgcccacggt
     2041 cgcgccgggc gcgccgatga gaatgtcgcc caggccgtcg ccattctggt cgccggcgta
     2101 gccgacggcg ccgcccgtct catccccggc ggcgctgccc tcgatgcgga agccgcgact
     2161 gccgtcgagc gcgccgagat tgagccgtgc cgggaagctg gccgcgccgt aaacgacgta
     2221 gaccacgccc accccgtcgg ccgcgcccac gcccgcgccg ggcgcgccca cgagcaggtc
     2281 agccaggccg tcgccgttca cgtcgccgcc ggccacggcg gtgcccgtgg cgtcgttgaa
     2341 cagcgcgccg gtcagacgaa agccattttt gccgtccagg ctggtcagat tgagcgacgt
     2401 tgggttcgtc gttgtgccga agacgacgta ggcttcgccc gcgcgttgca tgccatcggg
     2461 cgcggcctgg gccgcgccaa tgatgatgtc gttcagcccg tcgccgttca cgtcgccggc
     2521 gcttctgagc gcgcggccgg cctcgctgcc ggcggcgcgg ccgtcgatgc ggaagccgtt
     2581 ggggccgttc agcgcgccca ggctgaaggt agctgtggcc gcgggctgct cggcggcccc
     2641 ggcagcccgt ggccagagcg tcgccagcat cgccagcaac agcaacgccg ccagcggacg
     2701 aacgaagcca acgcgccggg gcgtgttggc cgacattggg aagaggggaa gttttgggtg
     2761 catggttgtg ccttgctggg tgggctttgt gtcgccccgc gcatggtcgc cgtcagaaat
     2821 ggtagcgcaa tgagcggcgc gcagccaact tcaacggccg gcgtggcggg cgagagtgtg
     2881 ccttgcggcc ggcttatggc ataatccccg tgcgccccga tccctgcggg tcagcgcgcc
     2941 cggatagcct catgagcgaa tttgccgtcg aactgcgccg cgaatcgaat cgccgctcgc
     3001 ccggacgctg ggttctgtcc cacttcagcc gccacggcgg gctgtttctg atcatgttca
     3061 tcggcgcgtt cggcaatgcc gctctggccg cggtgatgcc catctttatc ggcatcgcct
     3121 tcaacgcggc cctggccgat ccgcccgact ttcaacgtat tgccatcgcg gccatcgtca
     3181 tcctcgccag ccagatcgcg cggtcggtgc ttcagtttgg tcgcaacttc gccagttccg
     3241 tgatcgccga acgcatcgaa cgcgacagcc gccaggagct ttacctgagc ctgctgggca
     3301 agagcatgac cttccacgac ctccagccgg tgggcgacac gatggcgcgg gcgaccaacg
     3361 atgtgcatga gttgaacctg atgatgatgc cgggcgtcaa tctggtcgtc ggctcggcca
     3421 acttcctgat catgccgctg attctggccc cgcgctatga catgcagttg atcgccgcgc
     3481 cgctgttctt catcgttacc tacgtcctgg ccctggcccg ctacatgaat atcctgtcgc
     3541 gcatcgccga ccgcgtgcga cgcgccttcg gcgtcatgaa tgcgcgtctg gccgaggcac
     3601 tggatggcgt cgagatggtc aagggcagtg cccaggaatc gggcgaggtc gcgcgcttcg
     3661 aggccaacgc ccgtgaatat cgccacgcga ccgtggccca ggggcgcgag gaagccaagt
     3721 tcctgccgct gctgctgttg ggcgtcacgc aggcgattgg cttcacccac gccctgttgc
     3781 tgttacaggc cggccgcctc caggtgggtg acgtggttgc ctatgtaggc ctgctgagcc
     3841 tgtttggctt tccgaccaac atctccctga tggcctattc ccgcgtctcc ctgggcctgg
     3901 ccagtgcgcg gcgcatcctc gacctggtca atcgcgagac ccagctggac gagaatccgg
     3961 agggctacgc cgaacccatc gttggcgaca tcacttttga gggcgtttcc ttcggctacg
     4021 tgcccaacgt ggacgtgttg cgcgacgtcc gcttcaccgt ccgcgccggg cagaccgtgg
     4081 ccattgtcgg ccagaccggg gccggcaaga gcaccctgac caagctcatc aaccgcacct
     4141 acgacgcccg cgaggggcgc gtcctggtcg atggcgttga cgtgcgcgac tggagcctgg
     4201 ccgcgctgcg gcggcaaatt tctatcatcg agcaggacat cttcctcttc tcgcgcacca
     4261 ttgccgagaa catcgccttt ggcgcgccgg aggccacggc cgaggagatc gaagccgccg
     4321 cccgcgccgc tcaggcccac gatttcattg tcggctttca ggatggctat cagaccgtcg
     4381 tcggcgggcg cggcgtcacc ctgtcgggcg gccagcggca gcgcatcgcc ctggcccgcg
     4441 cgttcctaac ccagccgcgt atcctcatcc tcgacgactc caccagcgcc gttgacagtg
     4501 ctactgagga ccagattcag cgggccatcc agcacgccgc ccagaaccgc accacctttc
     4561 tgatcaccca ccgcctgtcc cagattcgct gggccgatct ggtgctggtc atgcgccaag
     4621 ggcggctaga ggcggccggc gaccacgcca cgctgatggc tgtttcgcct gcctaccggc
     4681 gcatcttcga ccagtacgag gcgctggaag gctaagggcg atgggaccgc tggtctacta
     4741 ctgccgctgg cggggcgtcc aactgcgctt gcgcggccgc gacgagagtg ccgtctgggg
     4801 ccagctggtc gagcgccggc gcgaagacgg cagggagcgg gccgaaccct tccgctttga
     4861 cctgcgcacc tgggaactgt ggctgggcga gccgcccggc cggcggctgc aactggatga
     4921 gttgggcgtt gtggaaagcg aggaaccctc gccggacgtt agttccagtg ctgacgactg
     4981 gcctcgaaca cgttgggaag attcttcaga cgtgtcaaca gatagctgag ctgggtcagg
     5041 tctttgacct cggcgatcat gtagagcgtg gttacagtat ctttggtgat catcttggtc
     5101 ttgggcacgt tgacctgctc gccgcgcagc aggttgataa tgtcttcgat caggccgctg
     5161 cgctgcacgg cctttacgac aatcgaaacg ggatgcgtct ccgcctcgac gccccattcg
     5221 acctcgataa gccgctcgcg gtcggtgata gccgccacct gcttgcagtc gcgggcgtgg
     5281 atggtgacgc cctggccacg ggtgatgtag ccgacaatcg gctcgggggc cacgggcgag
     5341 cagcaatgcg ccaggcgtgt atataggccg ccgacgccgc ggacggtcag accgcttttg
     5401 ggtttgtcgc tggggggctg caagaggggc cgcaaatcag cgtgttcgtc ggcgctcaga
     5461 gcttcttgct cttgcttgcg ccggctctca tactgcaggg cggtaatcgc cccgatgatg
     5521 cgctttgaat ggatgtcgcc gaagccgatc cgggagaggc aatcctcgac gctgccgagc
     5581 ttcaacgctc cggcgatgtc ctcgacagta tagacgtcgt gcaggaacag ttgccgcagc
     5641 tctctctgga caacctcgcg cccttgctgg atattctgtt cgcgctcctg ttggcggaac
     5701 cagtgacgga ttttgctgcg tgtacgggcg ctgcccgtat agcccaggga gggattcatc
     5761 cagtcgcggt tgggcgcgct gcgtttgagg ctatcgggct tgggggcgat aatatcgact
     5821 ctgtcgcctg atttcagacg gtagctaagg ctgaccggct tgccgttgac gcgcgcgccg
     5881 cggcagcggt ggcctaactc ggtgtggatc tggtaggcga agtcgatggg cgtggccccg
     5941 gcgggcaagt cgatgacgtc gtctttgggc gtgaagacgt ggatgcgctc ttccagccgg
     6001 gagatatccg gcaggggcgt gccctcgggg tcatcttcca cttcctggag tgtggccagc
     6061 agctcgcgca gattctggat gcgcttctgg ccagaagaag tgaccttggc gccttgctcc
     6121 ttgtaggccc agtgggcggc gatgccgtgc tcggcctctt cgtgcatccg ctgggtgcgg
     6181 atttgcacct ccagcttctg gcctgtctcg gcatcaatga tcgccgtgtg gagcgacatg
     6241 tagccgttgg ccttgggcga gccgatgtag tcatcgaatt cgccccggat cggctgccaa
     6301 aggctatgga cagcgccaag cgcctggtag cacagggcct gatccatgtc ggcgcgagcc
     6361 ttgctgctca gggccgcgta ggcgtccttg tccggcggct caaggatgac gcgcagggcc
     6421 tgcacgtcgt ggatctggtc gaaactcaac tccttgcgcg ccatcttgcg gtagatggag
     6481 taagtatgct tcggccgccc ggtgacgttc gccttcaggc ccaggctgtc cagccgccgc
     6541 cgcagacgcg ccgcggcctt gttgatcttg acggcccgga tgtcgcggcc gtcgtccagg
     6601 cggcgggcga tctcccgata cttctccggc tccaaatagc ggaaggccag gtcttcgagc
     6661 tgccacttga gatgccagat gccgaggcgg ttggcaatgg gggcgtacac gtgcagggcc
     6721 tcgaaggcga tgcggcgctg cccgtcgcgg tcaatgctgg cggcgcggcg caggtcttgc
     6781 agacaatcga ccatgcgaat gaggatgata cggatgtcgc cctcaatgat cgatagcagg
     6841 gcgcggcgga cgacttcgag ggtgcggcgg tcctgggtgg cgtcgagatt aagttcgtcg
     6901 gcggggcggc tgcggccggc gcgttgcgca tagtcgtcta acgtgttgac gctcttgacg
     6961 aggtcggcca cggtctcgcc aaactgcgcg cgcaacatcg cctcgctgat gccggtgtgg
     7021 ggcagcagga tgtcgtgcag cagggcggcg gcaatggcgt cggtctcgtc aatgcccaac
     7081 tcgcccagcc cggcagcgac ggcgcgggca tgatccaggt aggtatcgcc caacggccgc
     7141 gcgcggtcgc cgtgggcctt atcggccaac gacaaggccc ggcccaatcg cgccgagtcc
     7201 gactcgcttg tctggccggg gggcctgcct tttggcaggc ccccgcgggc gtcctgtgtt
     7261 gtctgaatca tgggttgttc cgtcggggcg cgcccggaca tggtcgcccg cacagcactg
     7321 ccggccgaga aaaacgcgcc taaacgaaca cgatcgaagg atggccccgt caccgcggcg
     7381 acgctagggc cacggccaga cctggcccta gccgctgctg ttcaggctgt cggtggcgtc
     7441 ctgggcagcg tcgcgacccg cgtcgaggac gatgcgtgtc tgttcggtgg cctggtcggc
     7501 gaaggtctgg gcgcggttgc gcgtgtcgga gatgacggcg ctggcccgat cgcgatattc
     7561 gcggctatag gtg
//
LOCUS       DAOSMQ010000094         7571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9700_length_7571_cov_5.229643, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000094 DAOSMQ010000000
VERSION     DAOSMQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9700_length_7571_cov_5.229643"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PK829_04595"
     CDS             <1..383
                     /locus_tag="PK829_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40525.1"
                     /translation="ARMLYLPLLILLMMLATAGISLWFAAMSVHYRDVKHAMPFLTQI
                     LMYAAPVVWSAALIEERFGQVGRLVFGLYPMVGVIEGFRVALLDTGPMPWDMLAAGTV
                     SALLLFVTGLFYFRRMEVNFADVA"
     gene            401..1717
                     /locus_tag="PK829_04600"
     CDS             401..1717
                     /locus_tag="PK829_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40526.1"
                     /translation="MNHSPNGQRAPVEAIVAENLAKQYHIGLREQRHDTLMQSTLATL
                     SAPLTNLRRLRRLGEVSDDEAADVLWALRDVSFRVAAGEVVGLIGRNGAGKSTLLKIL
                     ARITPPTRGRVVLNGRVSSLLEVGTGFHPELTGRENVFLNGTILGMRKAEVERRFDEI
                     VAFAEVDKFIDTPVKRYSSGMRVRLAFAVSAHLQSEILLVDEVLAVGDAAFQQKCLRA
                     MDGIAQTGRTVLLVSHNMQVIQQLCGRALLFEGGRLVADGAPSAVVARYLSHAATASL
                     PEPVRLGDELCLRQLSASQDGAPAGEWLDSGRPFELRVCYDILQSVRNLLLGFNVVSS
                     DGVNLFRTYDMQSAGLGERAPGRYESVFTLPGGLLPAGHYFFEVTVGLHRLRWLSRGD
                     IRLRLNLGGGRQTDVDFPGVVAPLGDWTVRRASEAREPIGSEQSHG"
     gene            1710..2522
                     /locus_tag="PK829_04605"
     CDS             1710..2522
                     /locus_tag="PK829_04605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU40527.1"
                     /translation="MAKFYWLAHERQVANGRMRWHAEEADETFWADYWESKVNAAYYR
                     QAEALDLAADELGGPLLAEMPRDGRHLEAGCGAGYWVAALRRAGLAVEGIEYSRELVT
                     LVNRVYPGLPVAYGDALALDCPDNTYASYLSFGVVEHREAGPQPFLDEALRILRPGGK
                     LIITVPHLGRLRRLRGRLGRYQTAPSKPFFQYGFRAAEFGALVRAAGFEVEYSRPLYI
                     HRLLLEEVGPYRWLYDRGGSAAWRNWVRRLGGGRDGHMLLLVAHKPAGKSAA"
     gene            2548..4479
                     /gene="asnB"
                     /locus_tag="PK829_04610"
     CDS             2548..4479
                     /gene="asnB"
                     /locus_tag="PK829_04610"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOU40528.1"
                     /translation="MCGICGELQLGDRPARDEATIAHLSALMARRGPDDAGHWRDGAH
                     AWLAFRRLAILDLSPAGHQPMLTPDGRYAMVFNGEVYNFAALRAELERLGAGFRSTGD
                     AEVVLQALAHWGTAALDRLNGMFALGLYDRQERSLLLARDHAGIKPLYYLQSDAGIFF
                     ASQYDQIMRHPWAAGRPVSADALGLYLRLGYIPAPRALMEGTFMLPPGAWLRLDGDGR
                     VERGTYYRFPVYQKPELRGAAADEAVNAAVADAVRRQMVSDVPLGAFLSGGIDSPLVV
                     ANMRATGRPVRAYTIGASGDPADESADAAAYARQLGVANHVVEPFTPDRALDWLGTVV
                     EACGEPFADHSIFPTLLVSNLARRDMTVMLSGDGGDELFWGYAERFGSVLRSAPDFRE
                     GYWRRSARWGARRVLNAGQAHKNLRARDIGDWYRQKHTRLPAPWLSAIFENAPAWPDD
                     FDLFAYDGWEPDATAQWLRWNEFVGHLSMVLLKVDRASMANSLEVRVPLLDREVIDVA
                     CRVDWQSCLDLGTGLGKLPLRHALARHLPAQTTAKRGFQIPMAAWLRGPLGEVFRDAF
                     AGRRELLGLAIHRPALVDMLENHVSGQDDLSRGLWTLLSLALWHDRHFRPVDSSVVRP
                     AVATTISSTVATTTVPSVL"
     gene            4492..5325
                     /locus_tag="PK829_04615"
     CDS             4492..5325
                     /locus_tag="PK829_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOU40529.1"
                     /translation="MIGIKSNRNGETQRNGVAATVYHALRPTAEATLLNGRYRLRSEA
                     RPNHTGERDYALLQTLARGKQCIFDVGANVGLTMLVMAETMAADGRLVAFEASEKGCY
                     LIRDNAALNGLSARVQVVNALVSDRSGYALDFFGDAASGSASVIPGYLAHHRALRKAT
                     LALDDFVARTALLPEFIKIDVEGAELAVLAGLATTMRAVRPLIFVELHSWGDMTVPGT
                     AARLLPQLAEMGYHMIYLRTKTVVNDPAELAGRGRCHVLLCPDGRPLPDALATFDTTG
                     L"
     gene            5345..6556
                     /locus_tag="PK829_04620"
     CDS             5345..6556
                     /locus_tag="PK829_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU40530.1"
                     /translation="MKTAEMTTPDKTQDAAQARPRARRLGAWLPRLLPVVLLLVVLVP
                     FRGPLQAAVKRNLANRPYLLAAMRATQDAKPETDGLEPTSGVQARALAYVALQGDRPG
                     EGLHWLLLGATTADPAMLTRFEICRLLMGQGRIAEARNYCRGAVATAYFWLAQGIAAD
                     ERHAPEMAIAYFDMARTADPELLSAWERLGRAAFNIERYGEAVEAYEHVLATQAHPLA
                     DTFYQLGLSYLALERIDDARKTLEQGLEIYPYQRELHLALADTARATNDLVTAEEWYA
                     RLLQQRPDDAYAWAARGEVAMQRGLAKQATIYLEEATSLQPTDVGHWLSLAAAAAAAG
                     DSHEAAMAYDKALALQPGDVGLLLGAATFFAQSGEVARAHALYLQVLALEPDNPTATA
                     ALAVPVANPTP"
     gene            6575..>7571
                     /locus_tag="PK829_04625"
     CDS             6575..>7571
                     /locus_tag="PK829_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40531.1"
                     /translation="MQQTTATSTSNPALVASLTGAPGYLGDVLVSFGVDAPPPRRPAA
                     PWAAAGAAAAASRVWEAPPAADWRGAPVMRLAAGDWTAWLLGELYGAARTPADVLAVL
                     DGRLSPNALNGHFLLLAHDARAGEWHAWTNRLATFHAYYAHDGRRAALGTCFRAVAAA
                     ARPDLDWEALTGFCAYGFCPADRTFLADVRILRPATHYRFDARVRLLSAERYWQWHYE
                     PDHGRAYDATVDAFAERFQAVMDDLLADGHVALPISGGLDSRSTVAAVRPGSPVVERL
                     WAYSYGYGVDSVETRIAGQVAAARGLSFDAFTVRPYLFARLPRVLASVEGFQEVTQ"
BASE COUNT         1065 a         2635 c         2603 g         1268 t
ORIGIN      
        1 cggcgcgtat gctctacctg ccgctgctca tcctgctgat gatgctggcc acggcgggca
       61 ttagcctgtg gtttgcggcc atgtcggtcc actaccgcga cgtcaagcac gccatgccct
      121 tcttgaccca aatcctaatg tatgccgcgc cagtcgtctg gtcggcggcg cttatcgagg
      181 agcgctttgg ccaggtcggg cggctggtgt ttggcctcta cccgatggtc ggtgtcatcg
      241 agggtttccg cgtggctctg ctcgatactg gccccatgcc ctgggacatg ctggccgccg
      301 gcacggtctc ggcgctgctg ctctttgtca ccggactctt ttactttcgc cgcatggaag
      361 tcaacttcgc cgacgtcgcc tgacgccgcg cccctgtgcc atgaaccact cccccaacgg
      421 tcagcgcgcg ccggtcgagg ccatcgtcgc cgagaacctg gccaagcaat accacatcgg
      481 cctgcgcgag cagcgccacg acacactcat gcaatcgacg ttggccacac tgtccgcgcc
      541 gttgaccaac ctgcgccggc tgcgacggct gggcgaagtc agcgacgacg aggccgctga
      601 cgtgctctgg gccttgcgcg acgtatcttt ccgcgtcgct gcgggcgagg tcgtcggcct
      661 gattggccgc aacggcgccg gcaaaagcac gctgctgaaa atcctcgcgc gcatcacccc
      721 gccgacgcgc ggccgggtgg tgctcaacgg gcgcgtctcc agcctgctgg aggtgggcac
      781 gggcttccac ccggagctaa ccggccgcga gaacgtcttc ctcaacggca cgatcctggg
      841 catgcgtaag gccgaagtcg agcggcgctt cgatgagatc gtcgccttcg ccgaggtcga
      901 taagttcatc gacacgccgg tgaagcgcta ctccagcggc atgcgcgtgc ggctggcctt
      961 tgctgtctcg gcccacctgc aatcggaaat cctgctggtc gacgaggtgc tggccgtggg
     1021 cgacgccgcc ttccagcaga agtgcctgcg ggccatggac ggcatcgccc agacggggcg
     1081 cacggtgctg ctggtcagcc acaacatgca ggtcatccag cagttgtgcg gccgggcgct
     1141 gctgttcgag ggcgggcggc tggtggccga cggcgcgccc tcggccgtgg tggcccgcta
     1201 cctcagccat gccgccacag catcactgcc ggagccggtg cggctgggcg acgagctgtg
     1261 cctgcggcag ctgagcgcca gccaggacgg cgcgccggcc ggtgagtggc tcgacagcgg
     1321 ccggccgttc gagttgcgcg tctgctacga catcctgcag tcggtgcgca atctgctgct
     1381 gggcttcaac gtcgtctcca gcgacggcgt gaacctgttc cgcacctacg acatgcagtc
     1441 ggccgggctg ggcgagcgcg cgccgggccg ctatgagtcg gttttcacgc tgcctggcgg
     1501 cctcttgccc gcggggcact acttcttcga ggtcaccgtt ggcctgcacc ggctgcgctg
     1561 gctgagccgg ggcgacatcc ggctgcgcct gaacctgggc ggcggccggc agaccgacgt
     1621 cgattttccc ggcgttgtcg ccccgctggg cgattggacg gttcgccgcg ccagcgaggc
     1681 gcgcgagccg attgggagcg agcagagtca tggctaagtt ctactggctg gcccacgagc
     1741 ggcaggtggc caacggccgc atgcgctggc acgccgaaga ggccgacgag actttttggg
     1801 ccgactactg ggaatcgaag gtcaatgcag cctactaccg ccaggccgaa gcgctcgacc
     1861 tggccgccga cgagttgggc ggcccgctgc tggcggagat gccgcgcgac ggccgccatc
     1921 tggaggcggg ttgcggcgcg ggttactggg tcgccgcgtt gcggcgggcc gggctggctg
     1981 tcgagggcat cgagtactcg cgcgagttgg tgaccctggt caaccgggtc taccccggcc
     2041 tgcccgtcgc ctatggcgac gcgctggcgc tcgactgccc cgacaacacc tatgcgtcgt
     2101 acctgtcctt cggcgtcgtg gagcaccgcg aggccgggcc gcagccgttt ctggacgaag
     2161 cgttgcgcat cttgcggccg ggcggcaagt tgatcatcac tgtgccccat ctgggccgct
     2221 tgcgccggct gcgggggcgg ctgggccgct accagaccgc gccctccaaa cccttcttcc
     2281 aatatgggtt ccgcgcggcg gaattcgggg cgctggtgcg cgccgccggc ttcgaagtcg
     2341 agtacagccg ccccctctac atccaccgtc tgctactgga ggaagttggg ccataccgct
     2401 ggctctacga ccggggcggc agcgcggcgt ggcgcaattg ggtgcggcgg ctgggcggcg
     2461 gccgcgacgg ccacatgctg ctcctggtgg cccacaaacc ggccgggaaa tcagccgcct
     2521 agcggcgaca agggagacac aacgacgatg tgcggcatct gcggcgaact ccagcttggt
     2581 gaccggcccg cccgtgacga ggcgacgatt gctcacctga gcgccctcat ggcccggcgc
     2641 ggccccgacg acgccggcca ctggcgcgac ggcgcgcacg cctggctggc cttccgccgt
     2701 ctggccatcc tcgacctgtc gccggccggc caccagccca tgctgacgcc cgatggccgc
     2761 tacgcgatgg tcttcaacgg tgaggtctac aacttcgccg ccctgcgcgc cgagctggag
     2821 cggctgggcg ctggcttccg ctccacgggc gacgccgagg tggtcttgca ggcgttggcc
     2881 cactggggca cggccgcgct tgaccggctg aacggcatgt tcgccctggg cttgtacgac
     2941 cgccaggagc gcagtttgct gctggcccgc gaccacgccg gcatcaagcc gctctactac
     3001 ttgcagagcg acgcgggcat cttctttgcc tcacagtacg accagatcat gcgccacccc
     3061 tgggctgccg ggcggccggt ctcggccgat gcgctgggcc tctacctgcg cctgggctac
     3121 atccccgcgc cgcgcgcgct gatggaaggc acgttcatgc tgccgccggg ggcgtggctg
     3181 cggctcgacg gcgatggccg cgtcgagcgc ggcacgtatt accgcttccc ggtctaccag
     3241 aagccggagt tgcgcggcgc ggcggccgac gaggccgtca acgccgccgt agccgatgcc
     3301 gtgcgccggc agatggtcag cgacgtgccc ctgggcgcgt tcctgtccgg cggcatcgac
     3361 tcgccgctgg tggtagccaa tatgcgcgcc accggccggc cggtgcgcgc ctacaccatc
     3421 ggcgcgtctg gcgacccggc cgacgagtca gccgacgcgg cggcctatgc ccggcagttg
     3481 ggcgtcgcca accacgtcgt ggagccgttc acgcccgacc gcgctctcga ctggcttggc
     3541 acggtggtcg aggcgtgcgg cgagccgttc gccgaccact ccatcttccc gaccttgctg
     3601 gtctcgaacc tggcccggcg cgatatgacc gtcatgctct ctggcgacgg cggcgacgaa
     3661 ctgttctggg gctatgccga acgctttggc agcgtcttgc gcagcgcgcc cgacttccgg
     3721 gaagggtatt ggcggcgcag cgcccgctgg ggggccaggc gcgtcctgaa cgccggccag
     3781 gcccacaaga acctgcgcgc ccgcgatatc ggcgattggt atcgccagaa gcacacccgc
     3841 ctgcccgcgc cgtggctgtc ggccatcttc gagaatgcgc ccgcctggcc agacgacttc
     3901 gacctgttcg cctatgacgg ctgggagccg gacgcgacgg cgcaatggct gcgctggaac
     3961 gagttcgtcg gccacctgtc gatggtcttg ctgaaggttg accgggccag catggctaac
     4021 tcgctggagg tgcgcgtgcc gctgctcgac cgcgaggtga tcgacgtggc ctgtcgggtg
     4081 gactggcagt cgtgcctcga cctgggtacg ggcctgggca aactgccgct gcgccacgcg
     4141 ctggcccgcc acctgccggc gcagacgacg gccaagcgcg gcttccagat tccgatggcc
     4201 gcctggctgc gcgggccgct gggcgaggtc ttcagggacg ctttcgccgg ccgccgcgaa
     4261 ctgctggggc tggccatcca tcggccggcc ctggtggaca tgctggaaaa ccacgtcagc
     4321 ggccaggatg acctgagccg cggtctgtgg acgctgctca gtctggcgct gtggcacgac
     4381 cggcatttcc gcccggtcga ttcttccgta gtccgtccgg ccgtggccac cacaatatcc
     4441 agcacggtgg cgaccacgac tgttccgtct gtgttgtaat ggaggcgaag tatgatcggt
     4501 atcaagtcca acaggaatgg ggagacgcaa cgcaacggcg ttgccgcgac cgtctatcac
     4561 gccctgcggc cgacggccga ggcaaccctg ctcaacggcc gctaccggct gcgcagcgaa
     4621 gcccggccaa accacaccgg cgaacgggac tatgccctgt tgcagacgct ggcgcggggc
     4681 aagcagtgca tcttcgatgt cggcgcgaac gtcggcctga cgatgctggt catggccgag
     4741 acaatggctg ccgacggccg cctggtcgcc tttgaggcct cggagaaggg ttgctatctc
     4801 atccgcgaca acgccgcgct caatggactg tccgctcggg tgcaggtcgt caatgccctt
     4861 gtgtccgacc gctctggcta cgcgctcgat ttcttcggcg acgcggcctc gggcagcgcg
     4921 tcggtcatcc ccggctatct ggcccaccac cgcgcgctgc gcaaggcgac gctggcgctg
     4981 gatgactttg tggcgcggac agccctgctg ccggagttca tcaagatcga cgtagaaggc
     5041 gcggagttgg ccgtcctggc agggttggcg acgaccatgc gtgccgtccg gccgctcatc
     5101 ttcgtcgaac tgcattcctg gggcgacatg actgtgccgg gcacggcggc caggctcctg
     5161 ccgcaactgg ccgagatggg ctaccacatg atctacctgc gcaccaagac ggttgtcaac
     5221 gatccggccg agctggccgg gcgggggcgc tgtcacgtgt tgctgtgccc ggacggccgg
     5281 ccgttgccgg acgcactggc aacgttcgac acaacgggcc tctagccgtc tgatcggagg
     5341 aatgatgaag acagccgaaa tgaccacgcc ggacaagacc caggacgccg cgcaggcccg
     5401 gccgcgcgcg cgccgtctgg gcgcgtggct gccgcggctg ctgccagtgg ttctcttgtt
     5461 ggtcgtcctg gtgccgtttc gcggcccgct gcaagccgcc gtgaaacgca atttggccaa
     5521 caggccatat ctgctggcgg ccatgcgcgc gacgcaagac gccaaaccgg agacagatgg
     5581 cctggagcct accagcggcg tgcaggcccg ggcattggcc tatgtcgcgc tacagggcga
     5641 tcggcctggc gaggggctac actggctgct gctgggcgcg acgacggccg atcccgccat
     5701 gctgacgcgc ttcgagatct gtcgtctgct gatgggccag gggcgcatcg ccgaggccag
     5761 gaactattgc cgcggcgctg tggccaccgc ttacttctgg ctcgcgcagg gcattgccgc
     5821 cgacgagagg cacgctcccg agatggccat cgcctacttc gacatggcgc gcacagccga
     5881 tcccgagcta ttgtcggctt gggagcggct cggtcgcgcc gctttcaaca tcgagcgcta
     5941 tggcgaggca gtcgaggcct atgagcacgt gctggccaca caggcccacc cactggccga
     6001 taccttctat caactgggcc tgtcctacct ggctctggag cggattgacg acgcgcgcaa
     6061 gactttagag caggggctgg agatctatcc ctaccaacgg gagcttcatt tggctctggc
     6121 cgacacggcc cgcgcgacca atgatctggt cacggctgag gagtggtacg cgcgtctctt
     6181 gcagcagcgg cctgatgatg cctacgcctg ggccgcgcgg ggcgaggtgg ccatgcagcg
     6241 tggcctggcc aagcaggcga ccatctatct ggaggaggcg accagcctcc agccgacgga
     6301 tgttggtcac tggctgagcc tggcggccgc ggccgccgcg gccggcgact cgcacgaggc
     6361 ggccatggcc tacgataagg cgctggccct acagccgggc gatgtcggcc tgctcctggg
     6421 tgcggccaca ttcttcgcgc agtcggggga ggttgcccgg gcccacgcgc tctacttgca
     6481 ggtgctggcg ctggagccgg acaaccctac cgcgacggcc gcgctggccg tgccggttgc
     6541 caaccccaca ccctagccgt gatggagtct tgcgatgcaa cagacaacag caacctccac
     6601 atctaaccct gcgctggtcg cgtctctgac gggtgctccc ggctatctgg gcgatgtgct
     6661 ggtgagcttc ggcgtcgacg ccccgccgcc gcgccgcccg gccgcgccct gggccgctgc
     6721 cggcgcggcc gccgcggcaa gccgggtctg ggaagcaccg cccgcggccg actggcgcgg
     6781 cgcgccggtc atgcgcctgg ccgcgggcga ctggacggcc tggctgctgg gcgagttgta
     6841 cggcgcggcg cgcacgccgg ccgacgtgct ggcggtgctc gacggccggc tgtcgcccaa
     6901 cgcgctgaac ggccacttct tgctgctggc ccacgacgcc cgcgccggcg agtggcacgc
     6961 ctggacgaat cgcctggcca ccttccacgc ctactatgcc catgacggcc gccgggcggc
     7021 gctgggcact tgtttccgcg ccgtcgccgc cgccgcgcgg cccgatctgg attgggaggc
     7081 gctgaccggc ttctgcgcct atggcttctg ccccgccgac cgcacctttc tggccgatgt
     7141 acgcatcctg cggccggcca cccactaccg ctttgacgcc cgcgtccgcc tcctttcggc
     7201 cgagcgctac tggcagtggc attacgaacc cgaccacggc cgggcctacg acgccacggt
     7261 tgacgccttc gccgagcgct tccaggcggt catggacgac ctgctggccg atggccacgt
     7321 cgccctgccc atctccggcg gtctggattc gcgctcaacg gtggccgcgg tgcggcccgg
     7381 cagccctgtc gtcgagcgcc tgtgggccta ctcctacggc tacggcgtcg attcggtcga
     7441 gacgcgcatc gccgggcagg tggccgcggc ccgcgggttg agcttcgatg ccttcaccgt
     7501 gcggccctat ctcttcgccc ggctgccgcg cgtgctggcc agcgtggagg gcttccagga
     7561 agtcacccag g
//
LOCUS       DAOSMQ010000095         7565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9713_length_7565_cov_4.014647, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000095 DAOSMQ010000000
VERSION     DAOSMQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9713_length_7565_cov_4.014647"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PK829_04630"
     CDS             <1..1250
                     /locus_tag="PK829_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOU40532.1"
                     /translation="AVVAFFKQLGVSRRLSILLEGESLFNDGVAVVIFNLAIAAAATM
                     LVSNAAVSFNFLGAVGEFLRVSLVGLGVGLLLGFAVAYLVLRSIDDHLIETATTVALA
                     FGAYVAAEQLHASGLLAVVAAGLVVGNVGMDRTSPTTRLTLDNFWEFMAFVANSLVFL
                     LIGIRIQLGQLVQFAVPIGVAVLAVLFSRAISIYLLTALHNRLTPERLDVSQGYRHVM
                     FWGGLRGAISLALALTIGGNLFGQTIATEIQVMAFGVVLFTILVQGTTIEAVINRLGL
                     VQKQPAAIENQRRLAVVYARQAGRRELNRLHAEGFLYHDLWQAMNSVYDEEIDDARAA
                     LRDHLEMHPELERSMYLQTRADVVKAERNAISAAAARAFISAKVQEELLIEMDARLAA
                     LDQLLADDRGSLSGHGPAEGEDG"
     gene            1243..1683
                     /locus_tag="PK829_04635"
     CDS             1243..1683
                     /locus_tag="PK829_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU40533.1"
                     /translation="MADIICAIRAGEGSRAVQNAAIRAALSQNDRLLFLYVVDQRLVE
                     ASDESIRPSARAELYWMGRTLLRIAVARAHAAGLRDATWAIREGAVGQEIAGVVVEHG
                     ARVLFMGASRHAGGRPAAAKSLADHLRDTIGVAVDIVSPSLEDA"
     gene            1726..3528
                     /locus_tag="PK829_04640"
     CDS             1726..3528
                     /locus_tag="PK829_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HOU40534.1"
                     /translation="MSKIELNDEQQAMLAGERGRARQMGMRLLLDLAYMTEAERLVAI
                     ESAHLSGVSPLTGGLGLRQFLARLAAEAGPGVAVPTTLNSAGCDEAQFPAMRIAEPNF
                     LEHHREIIASYARLGVHTIQSCVPYEWEGIPPSRPGAVVAWAESNAICYANSYGGLRT
                     NRESGLSALASALTGYAPDYGLLDEAARRPNLAVTVTAPLDDPTDYSILGDWIGKQRR
                     PGWRMPYGPIPAISGLPAVLTHEQRKALSAAAANYGCPLLYVEDTPAGDYQDHLTFGP
                     AELARRYEELRPRGPVSLVVLGCPQASLGELAAIARLLAGRRVASEPTAAGNPPLWVF
                     TSAAAKAVTERNGIAAAIRGSGALLLENTCPEVIPYDQSWVHAILTNSMKAEHYIKSG
                     LNGVPTAVMRLADCIAVAAGELRVAGELQRDDAPPAAGIATGSSAPATRAPAPSPPTA
                     PTEADSAPFHSRGRGLPSQGDFTVEGEALVTDTPITFLGYVNRLTGVIEEEGHPANGQ
                     SLAGKVAIFPRGTGSSVAPYVLLELHYRGVGPLAVVNSEIDQQTVPACSLEGLPYAYG
                     FDADVLHGVRGGDRVRLARRGQSVTLEVLEAAHE"
     gene            3521..4750
                     /locus_tag="PK829_04645"
     CDS             3521..4750
                     /locus_tag="PK829_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU40535.1"
                     /translation="MNSELLNFLASVVIVIVAAKVGGYLSIRFGQPSVLGELLVGILL
                     GPTVLNMLGAWPLFAGDSHLSASLTLFAEIGVILLMFLAGLELELGDLLRSGRVAGLA
                     GMLGVVVPLAGGYATARLFGLDNVEAVFIGLALSATSVSISAQTLIELGVLRSKVGLG
                     LLGAAVFDDILVVLLLSGASVVFGSEGGAGGILMVLLRMVLFLAGASVVGLFVVPRLM
                     TLVEELPISQAVPTAALVICLLFAWASEALGGMAAITGAFMAGLFFARTDYVHHIETS
                     LAAMAYSLFVPIFLVNIGLQANLRAISGTGWYLAIALTIVAILTKVFGSGLGALAGGF
                     NRRDSLRLGVGMISRGEVGLIVASVALRAELIHPSTFSGIVFMVIVATLVTPPLLRLL
                     YRDDVSTSAGVETAANS"
     gene            4769..5140
                     /locus_tag="PK829_04650"
     CDS             4769..5140
                     /locus_tag="PK829_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40536.1"
                     /translation="MYYTVFLVLDNLEHESALLEGWENAGVTGITILDSTGLGRIRQR
                     ASLRDDMPLLPSLHALLHAKEERHRTFMSVVDGEEMVNGIIEATQAILGDMSLPNTGI
                     LFVMPVTRVVGVPRRGQTDVP"
     gene            complement(5173..5246)
                     /locus_tag="PK829_04655"
     tRNA            complement(5173..5246)
                     /locus_tag="PK829_04655"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5210..5212),aa:Val,seq:cac)
     gene            5305..5958
                     /locus_tag="PK829_04660"
     CDS             5305..5958
                     /locus_tag="PK829_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40537.1"
                     /translation="MIVQTAQLHSRRAWLLALLILLLVAGCRGGGSNLPWEETFNEAG
                     SWSTGEDAYSRGNVSDGAYVFEVLQNDISRWAAAGQNFGDGIYELEATQVSGPLDNGY
                     GLLFRANAEAGNFYLFKVSGDGYVWLGRYQDGAEAATLVGDHWFESAAVQQGLNVTNR
                     LTVRAEAGNMIFYVNGQEVGRVTDNTLASGDVGVLVQTLGGAPVVVRFDGLTVKALD"
     gene            6203..>7565
                     /locus_tag="PK829_04665"
     CDS             6203..>7565
                     /locus_tag="PK829_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOU40538.1"
                     /translation="MLETQAKKTERQEPEQQLAISAAEVLRDYSLAVQSRQASLVGRR
                     EVLSGKAKFGIFGDGKELAQIAMAKAVRPGDFRTGYYRDQTFIFALGATTIQQFFAQL
                     YAHPDVLAEPNSAGRQMNSHFATRLLDEDGRWLDQTQQFNSAADLSPTASQMPRLVGL
                     GYASRLYRELDDLKAMTGFSRNGNEVAFGTIGNASTAEGHFWETVNAIGVLRLPVVIS
                     IWDDEYGISVPNVYQITKGNLSEVLSGFRREAEGEPGFEIFTVKGWDYLALIETYARA
                     AELARQEHVPVLIHVIEMTQPQGHSTSGSHERYKSPERLAWEQEYDCLRKMRAWILEQ
                     RLAPAGELDNIERNAEKLVENLRMKAWQAFTRPIYDERQEAARLMEAVETQSPQAEAV
                     AAVRRRLLAKETVLRRDVLIAVGEALAATRGEPRGPETPVGRLLAWRQTQLAANRERY
                     SSHL"
BASE COUNT         1213 a         2554 c         2483 g         1315 t
ORIGIN      
        1 ttgccgtggt tgccttcttc aagcaactcg gggttagccg gcggttgagc atcctgctgg
       61 agggcgagag cctttttaat gatggtgtgg ccgtcgtgat cttcaatctg gccattgccg
      121 cggcggcgac catgctggtc agtaatgccg cagtctcctt caactttctg ggggccgtcg
      181 gcgaattcct gcgcgtctct ctggttgggc tgggcgtggg tctgctgcta ggtttcgctg
      241 tggcctatct cgttctgcgc agcatcgacg accatctcat cgagacggct acgactgtcg
      301 ccctggcgtt tggcgcctac gtggccgccg agcagctgca cgccagcggc ctgctggccg
      361 tcgtcgccgc cgggctggtg gtcggcaacg tcggcatgga ccgcacctcg cccacaacgc
      421 ggctgacgct ggacaatttc tgggagttca tggccttcgt ggccaactcc ctggtttttt
      481 tgctgattgg cattcggatt cagctcgggc aattggtgca gttcgccgtg cccatcggcg
      541 tggctgtgct ggccgttctg ttcagtcggg cgatcagcat ctacctgcta acggcccttc
      601 acaaccggct gacgcctgaa cggctggatg tctcccaggg ctaccggcac gtcatgttct
      661 ggggcggcct gcgcggggcc atcagtctgg ccctggcgct gaccattggc ggcaacctgt
      721 tcggccaaac catcgccacc gagatacagg tcatggcctt tggcgtggtg ctcttcacca
      781 tcctggtgca aggcacgacc atcgaggccg tcatcaatcg gctggggctg gtgcaaaagc
      841 agccggcggc catcgagaac cagcgtcgcc tggccgtcgt ctacgcgcgt caggccggcc
      901 ggcgcgagtt gaaccggctg cacgccgagg gattccttta ccatgacctg tggcaagcga
      961 tgaatagcgt ctatgatgag gagatcgacg acgcccgcgc cgcgctgcgc gaccatctgg
     1021 agatgcaccc ggaactggag cgctcgatgt acttgcagac gcgggccgat gtcgtgaagg
     1081 cggagcgcaa cgccatcagc gccgccgccg ctcgcgcctt tatttcggcg aaagtgcaag
     1141 aggaactgct cattgaaatg gatgcccggc tggcggcgct ggatcagctt ctggccgacg
     1201 accgcggcag cctcagcggg catggcccgg ccgagggcga agatggctga catcatctgc
     1261 gccatccgcg cgggcgaggg cagtcgcgcg gtgcagaacg cggccatccg cgccgcgctc
     1321 tcccagaatg acaggctgct cttcctgtac gtcgtcgacc agcgccttgt ggaggccagt
     1381 gacgagagca taaggccgtc cgcgcgcgct gagctatact ggatgggccg gactttgctt
     1441 cgcatcgccg tcgcccgcgc ccacgccgcc ggactgcgcg acgcaacctg ggccattcgt
     1501 gaaggagcgg tgggccaaga gatcgccggc gtggtggtcg agcatggcgc tcgggtgctg
     1561 tttatgggtg cttcccggca cgccggcggc cggccggccg cggccaaatc gctggccgat
     1621 catctgcgcg acaccatcgg cgtggccgtc gatatcgtct ctccgtcgtt ggaagacgcc
     1681 tgagcgagca cgcgccgggg cgatgaggag gatagaagag tacgcgtgag caagatcgaa
     1741 ctaaacgacg aacaacaggc catgctggcc ggcgaacgcg gccgggcgcg gcagatgggt
     1801 atgcgcctgc tgctcgacct ggcctacatg acagaggccg agcgcctggt ggccattgag
     1861 agtgcccact tgtcgggcgt gtcgccgttg accggtggcc tggggctgcg gcagttcctg
     1921 gcccggttgg cggctgaggc cgggccgggc gtggccgtgc cgaccacgct caactcggcc
     1981 ggttgcgacg aagcccagtt cccggccatg cgcatcgccg aacccaactt cctggagcac
     2041 caccgcgaga tcatcgcttc ctatgcccgg ttgggcgtcc acaccatcca gtcgtgcgtg
     2101 ccctatgagt gggaaggcat ccccccgagc cggcccgggg ctgtcgtcgc ctgggcagag
     2161 tcgaatgcta tctgctacgc gaactcctac ggcggcctac gcaccaaccg cgagtcgggt
     2221 ctgtcggcgc tggccagcgc cctgaccggc tacgcgcccg actatggcct gttggacgaa
     2281 gccgcccgcc ggcctaacct ggctgtcacc gtcaccgcgc cgctggacga ccccaccgat
     2341 tactccatcc tgggcgactg gatcggcaag cagcgccgcc ccggctggcg catgccctac
     2401 ggccccatcc cggctatcag cggcctgccg gcggttttga cccatgagca gcgcaaggcc
     2461 ctcagcgcgg cggcggccaa ctacggctgc ccgctgctct acgtcgaaga cacgcccgcg
     2521 ggcgactatc aggatcacct caccttcggc ccggccgaac tggcccggcg ctacgaggaa
     2581 ctgcgcccgc gcggcccggt ctcgctggtt gtcctcggct gcccgcaagc gtcgctgggc
     2641 gaactggcgg ccattgcccg gctgctggcg ggacggcgcg tggcgtctga gccaaccgca
     2701 gccggcaacc cgccgctgtg ggtcttcacc tcggccgcgg ccaaagccgt caccgagcgc
     2761 aacggcatcg ccgcggccat ccgcggcagt ggcgcactgc tgctggagaa cacctgcccg
     2821 gaggtcatcc cctacgatca gtcctgggtt catgccatcc tgaccaattc catgaaggcc
     2881 gagcactaca tcaagtctgg cctgaacggc gtgccgacgg cggttatgcg gctggccgac
     2941 tgtattgccg tggccgcggg cgagttgcgg gtcgcaggcg agttgcagcg agacgacgcg
     3001 ccgccggccg cgggcatagc gaccgggtcg agcgcgccgg cgacgcgcgc cccagcgccc
     3061 tcgccgccga ccgcgccgac cgaggctgac agcgcgccgt tccacagtcg cggccgtggc
     3121 ctgccctcgc aaggcgattt caccgtcgag ggcgaggcgc tggtcaccga cacgccgatc
     3181 actttcctgg gatatgtcaa tcgcctgacc ggcgtcatcg aggaagaagg ccacccggcc
     3241 aacggccaga gcctagccgg caaggtagcc atcttcccgc gcggcaccgg ctccagcgtg
     3301 gccccctatg tcttgctgga attgcactac cgcggcgtcg gcccactggc cgtcgtcaac
     3361 agcgagatcg accagcagac tgtgcccgcc tgctcgctgg aagggctgcc ctacgcctac
     3421 ggtttcgacg ctgacgtact ccacggcgtg cgcggcggcg accgtgtgcg tctggcgcgg
     3481 cgcggccaga gcgtaaccct ggaggtgctg gaggcggcgc atgaatagcg aactcctcaa
     3541 cttcctggcc agcgtagtca tcgtcatcgt cgctgccaag gtgggtggct acctcagcat
     3601 ccgcttcggc cagccgtcgg tgctgggcga actgctcgtc ggcatcctcc tcggcccgac
     3661 ggtgctcaac atgctcggcg cgtggccgct cttcgccggc gacagccatc tatcggcctc
     3721 gctgaccctc ttcgccgaga tcggcgtcat cctgctgatg tttttggctg ggctggaact
     3781 ggaactgggc gatctgttgc gctccgggcg tgtggccggg ctggcgggca tgctgggcgt
     3841 cgtcgtgccg ctggcgggcg gctacgccac ggcccgcctg tttggcctgg acaacgttga
     3901 ggccgtcttc atcggcctgg ccctctcggc caccagcgtc agcatctccg cgcagacgct
     3961 gatagaactg ggcgtgttgc gcagtaaggt cggcctgggg ctgctgggcg cagccgtctt
     4021 tgatgacatc ctcgtcgtcc tgttactgtc gggcgcgtcg gtcgttttcg gctccgaggg
     4081 gggtgcgggt ggcatcctga tggtcctgct gcggatggtg ctctttctgg ccggcgcctc
     4141 ggtcgtgggg ctgtttgtgg tgccccggct catgacgctc gtcgaggaac tgccgatcag
     4201 ccaggccgtg ccgacggcgg cgctggttat ctgcctgctg ttcgcctggg cgtcggaggc
     4261 attgggcggc atggcggcca tcaccggcgc gttcatggcc ggcctgttct tcgcgcgcac
     4321 ggactacgtc caccacatcg agacgagtct ggcggccatg gcctacagcc tcttcgtgcc
     4381 catctttttg gtcaacatcg gcctgcaagc caacctgcgg gccatcagtg gcacaggctg
     4441 gtacctggcc atcgccctta ccattgtcgc catcctcacc aaggtcttcg gcagcggcct
     4501 gggcgcgctg gccggcggct tcaaccggcg cgacagcctg cgcctgggcg tgggcatgat
     4561 ctcccgcggc gaagtcggcc tgatcgtcgc ctcggtcgcc ctgcgcgccg agctgatcca
     4621 cccgtcaacc ttctccggca tcgtctttat ggtcatcgtc gccacactgg tcacgccgcc
     4681 gctgttgcgg ctgctctatc gcgacgacgt gtccacgtcc gccggtgtcg aaacggctgc
     4741 caattcctag cggcaaaagg aagtatctat gtattacacc gtctttctcg tcctggacaa
     4801 cctggagcat gagagcgccc tgctagaggg ctgggagaac gccggcgtga ccggcatcac
     4861 cattctagac agcaccggcc tgggccgcat ccgccagcgc gccagcctgc gcgacgatat
     4921 gcccctcctg cccagccttc acgccctgct ccacgccaag gaggaacgcc accgcacgtt
     4981 catgtctgtc gtggacggcg aggagatggt caacggcatc atcgaggcga cgcaagccat
     5041 tctgggcgat atgagcttgc ccaacaccgg cattctgttt gtcatgccag tcacacgggt
     5101 ggtcggcgtg ccccggcgcg ggcagacgga tgtaccttag ggaacagggg ataagaagag
     5161 gacagacgaa ggtgggcgcg gagggactcg aacccgcgac ctcacggatg tgaaccgtgc
     5221 gctctaacca actgagctac gcgcccacgt ctagacagta gtataaccac ttgcgcggca
     5281 atcgccaagc cttcagagga ctttatgatc gttcagacag cacaacttca ctcccggcgc
     5341 gcctggcttc tggcgttgct catcttgctg ttagtggccg ggtgccgcgg cggcggcagc
     5401 aacctgccct gggaagagac cttcaacgaa gccggctcat ggagcacggg cgaggacgcc
     5461 tattcgcgtg gcaacgtcag cgacggcgct tacgtcttcg aggtgctgca aaacgacatc
     5521 agccgctggg ccgccgccgg ccagaacttc ggcgacggca tctatgaact ggaagccacc
     5581 caggttagcg gcccgctcga caatggctac ggcctgctct tccgcgccaa cgccgaagca
     5641 ggcaacttct acctcttcaa ggtctccggc gacggctacg tctggctcgg ccgctatcag
     5701 gacggggcgg aggcggctac gctcgtcggc gaccactggt ttgagtcggc cgccgtccag
     5761 caggggttga acgtcaccaa ccgcctgacc gtccgcgccg aggcgggcaa catgatcttt
     5821 tacgtcaacg ggcaggaggt cggccgcgta accgacaaca cgctcgcttc cggcgacgtc
     5881 ggcgtactgg tgcagacgtt gggcggcgcg ccggtcgtgg tgcgcttcga tgggctgact
     5941 gtcaaagcat tggattaggc tgcgcctaaa gggttgctct ggcgctacgc ctttggccgt
     6001 tttgtgctat gatgctggcg gatggcgtac ccaccgccac cgcggggccg aacgacggcc
     6061 cgcgccaatc tgaaccgagt gccgcgccaa acgaccggcc acgctatcat cgccgcccgc
     6121 gacgcagacc attctgcgtc gcttttgtga gtggtacggc ccggcgggcg cggagaacat
     6181 aaccgtgcgc gaagggcaag caatgctgga aacacaagcg aaaaagacgg aacgacagga
     6241 gccagagcag cagctggcca ttagcgcggc cgaggtgctg cgcgactaca gccttgccgt
     6301 ccagagccgg caggccagcc tcgtcggccg gcgcgaggtc ctcagcggca aggccaagtt
     6361 cggtatcttc ggcgacggca aggagctggc ccagatcgcc atggccaagg ccgtccggcc
     6421 gggcgacttc cgcaccggtt actaccgcga tcagacgttt atcttcgccc tgggcgcgac
     6481 gaccatccag cagttcttcg cccaactcta cgcccacccc gacgtcctgg ccgaacccaa
     6541 ctccgccggc cggcagatga acagccactt cgccacccgc ctgctggacg aggacggccg
     6601 ttggctcgat cagacgcagc aattcaactc cgccgccgac ctctccccga ccgcttcgca
     6661 gatgccgcgc ctggtcgggc tgggctatgc ctcgcgcctc taccgcgaat tggacgacct
     6721 caaggccatg accggcttct cgcgcaacgg caacgaggtc gccttcggca ccattggcaa
     6781 cgcctccacg gccgagggcc acttctggga gacggtcaac gccatcggcg tgctgcgtct
     6841 gccggtggtc atctccatct gggacgacga gtatggcatc tccgtgccca acgtttacca
     6901 gatcaccaag ggcaacctgt ccgaagtgct gtctggcttc cgccgcgaag ccgagggcga
     6961 gccgggcttc gagattttca ccgtcaaggg ctgggattac ctggccctga tcgagaccta
     7021 cgcccgcgcc gccgagttgg cccgccagga gcacgtgccc gtcctcatcc acgtcatcga
     7081 gatgacccag ccgcagggcc actcgaccag cggcagccac gagcgctaca agtcgccgga
     7141 gcggctggcc tgggagcagg agtacgactg cctgcgcaag atgcgcgcgt ggattctgga
     7201 gcagcgcctc gcccccgcgg gcgaactgga caacatcgag cgcaacgccg agaagctggt
     7261 ggagaacctc cgcatgaagg cctggcaggc gttcacgcgg ccgatctacg acgagcggca
     7321 ggaagcggcg cggctgatgg aagcggtcga aacccaatcg ccacaggccg aagccgtggc
     7381 cgccgtccgt cgccggctgc tggccaaaga gacggtgttg cgccgggacg tgctcatcgc
     7441 cgtgggtgag gcgctggcgg ccacgcgggg cgaaccgcgc ggcccggaga cgcccgtcgg
     7501 ccggctgctg gcctggcggc agacacaact ggcggccaac cgcgagcgct atagctccca
     7561 cctct
//
LOCUS       DAOSMQ010000096         7516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9802_length_7516_cov_4.454765, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000096 DAOSMQ010000000
VERSION     DAOSMQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9802_length_7516_cov_4.454765"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PK829_04670"
     CDS             <1..444
                     /locus_tag="PK829_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU40539.1"
                     /translation="PAVCIYECPVRVFRECQNLPKRFDLIAAPRLDHPLMPPPPAIAP
                     RDLWQTLRTTPAGGQPIVLDVRESREFRRSHIAEAQSTPLSTLLAGGVALPAGRSVVL
                     VCQTGRRSRRAAAVLRDLGFNDLTLLEGGMQAWEAAGLLTAVEFT"
     gene            446..1414
                     /locus_tag="PK829_04675"
     CDS             446..1414
                     /locus_tag="PK829_04675"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HOU40540.1"
                     /translation="MDTFLTSRNSGLDLVRVTEAAALAAGRWIGSGNFAAAHAAATDA
                     MFNMLAALQIDGHVAIGEDRLIDGAAPLAEGATFGAGLMPYDLAVDPIDGTRLLVEGK
                     PGAISVVAMAPRHSIRSIAPAEYLEKIVVDRDAAAVLVPECLNAPAAWTLALIARTKK
                     KAVRDLVVVVLQRARHHDLIEEIRATGARVLLREEGDAEGALVAATPGSRVDVLMGVG
                     GAAQGGLAACAVRALHGAMLAQVAPQSAEERAAVDQAGLGNHRVLTAEELVASDDVLF
                     AATGITDTSLLPGIAYRGSHAETHSLLLRAKTRTRRFIQAEHNLSE"
     gene            complement(1467..1919)
                     /locus_tag="PK829_04680"
     CDS             complement(1467..1919)
                     /locus_tag="PK829_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40541.1"
                     /translation="MATQQTDVSQNALSGFIRAAGVILAISYPVLALSTGARALYQLL
                     LKEGVTNFLPPALSGVAALCYLLATVGFIVRRKWAWRLSVSMLVFELAMVLLVGTLSY
                     IMPDTIGRTVWAHFGADYGFFPLIQPILGLAWLFHRETRAAYELGIRN"
     gene            1999..3480
                     /locus_tag="PK829_04685"
     CDS             1999..3480
                     /locus_tag="PK829_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40542.1"
                     /translation="MYDDDHPDTPPLRNDLTDLPEGEGGRRGCIFLGVTMLLIVSLIG
                     SSLLAWFVLVRREQTSAPPTPLVASTLAPATLVAALPTPQPIAAQPTAAPIDGTAINR
                     IVIVNPEGQVETLSPTGGDRRVLTREEDSVLFQFPAWSPDGRHLAVIGSRLTGGGVYV
                     LPDTPGGSDVSASQVYFSANETPIYLFWSPDSATLAFLANQSRSGMGLMVIDSAGASE
                     SRLLATGAPFYWDWSEDGRQLLIHTGADRGGDVSLIDVEGQVQAANVATPGQFQAPGI
                     APGGRYWAFAEETDGQSALVIVDTQTGERRSFEPSRSLALGWSPTGEQLAFTNATAAN
                     HPFWGPLRVLDAATGEARLLTRQTVLAFFWSPDGHAIAFITLNDSFNDDGVNALAKTR
                     TLARLASPAQQTAGFLTLSVVDVTTGQGLRLLDFEPTVVFLTQFLPFFDQYALSHRLW
                     SPDSRSLVLPVREEEGDVILIVPAAGGPPSFLAEGQAAFWSQR"
     gene            complement(3477..4697)
                     /gene="xseA"
                     /locus_tag="PK829_04690"
     CDS             complement(3477..4697)
                     /gene="xseA"
                     /locus_tag="PK829_04690"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU40543.1"
                     /translation="MFSFASQSTLDHTIWTVGELTHYVRQMFDVDYRLRDIDVSGEIS
                     NFTRAASGHVYFTLKDAGAQLKCVMWRSTAERLRFRPAEGDAVIAHGRVSVYEVSGVY
                     QLYVEHMQPAGRGDLAAAFERLKDMLAAEGLFDPERKQPIPPFPRRIGIVTSADAAAL
                     RDILIVLARRNPLVSVLIAPTLVQGELAAAQIVRALQRLDERDDIDVILLARGGGAIE
                     DLWAFNDERVARAIYAARHPIISGVGHETDFTIADFVADLRAPTPSAAAELAVPDLSG
                     AGPVLAEMRATLTAAIGSALVGRRQALRARAQWLTLLSPQRILDSDRQRVDMLAERLA
                     VNAARGLERRRGWLAVAGSSLAAFSPQATLARGFAIVRGPDGQLIRSVIQAQPGAALR
                     VQVSDGVFGATVDD"
     gene            4752..5132
                     /gene="rsfS"
                     /locus_tag="PK829_04695"
     CDS             4752..5132
                     /gene="rsfS"
                     /locus_tag="PK829_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU40544.1"
                     /translation="MVIYLVQGRCFLEGLELTQLLVDALLDKKGVDILVLDIHAQAIF
                     ADFFVICSGESEPQLRALSQAALESAKKEGGRSPKGVEGTPADGWVLVDFGDVVVHLF
                     SPQKRSYYDLESLWHNGRVVVRMQ"
     gene            complement(5194..5643)
                     /gene="ndk"
                     /locus_tag="PK829_04700"
     CDS             complement(5194..5643)
                     /gene="ndk"
                     /locus_tag="PK829_04700"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU40545.1"
                     /translation="MEQTLVLVKPDGVQRGLIGEIITRLERRGLKLLGLKLMQMTREL
                     AAAHYAAHEGKSFYNGLVDYITSGPIVAMVWEGKDAIAIVRKTMGATKAGEAEPGTIR
                     GDLAVEIGRNLVHGSDGAESAAREVALFFGEGELVSWERATDRWIRE"
     gene            complement(5676..>7516)
                     /locus_tag="PK829_04705"
     CDS             complement(5676..>7516)
                     /locus_tag="PK829_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40546.1"
                     /translation="GGEVAAPVFARVANRALASKLLREAGISPESLDLRGSTNEMSLG
                     DLSLLEGNTPINAFVAAEDFADTIVLMETMAADQGRTLEEPPPPATVSPAPPQATDLA
                     APATNEDSFEGAMAFLDELAAAQEPPGSTTQPIEPVEMTAGPAAGELALEQVVDRADK
                     EWAQPEWLTEPATETAATDAEALAQDSVELAEPIELAELTEPAEPAEQEAWPAEMVEP
                     AAATQAEAMLPEEAPWIELDIDRQPLSPAEDEPAGELPADMAMPATMARRNGDDGASL
                     EETLLALDALALPPGASLAEIDSSLRQSGGLSARRDLPAAVEWLELSLGLSGPLPAAP
                     LSDEDLIARMPEDPDAVLAWLEQMAEEDTVDSSPSLPVEEAAPAAAVANLAPSAGVNA
                     EPVLEELSEADLLNMPEDPDAIIAWLEGLAGNSAANPPEPSASPAAILAEEPATPETA
                     ADLGAPAALEAPVLESGAEEARPWPDVDTEAGLTEATPGDKAPAEAAVESAPAEGVTV
                     ATLPADARPRVAARPRRRGRRATPVVEATAAEAAREETTPAAPEAAEVSVADVTTVTV
                     EAATGIVAQEQATVEESEAAAAPKSEEPPAPPARPASWVDLLKPLR"
BASE COUNT         1150 a         2586 c         2479 g         1301 t
ORIGIN      
        1 ccggctgtgt gcatctacga gtgccccgtg cgcgttttcc gtgagtgcca gaacttgccc
       61 aagcgcttcg atctgatcgc cgcgccgcgg ctcgatcacc cccttatgcc gcctccgccc
      121 gccattgctc cgcgcgatct gtggcagacg ttgcgcacga cgccggccgg cggccagcca
      181 atcgtgctcg acgtgcgcga gtcgcgcgaa ttccggcgct cccacatcgc cgaggcgcaa
      241 tcgacgcccc tatcgacgct gttggccggt ggggtggcgc tgccggccgg ccggtcagtg
      301 gtgcttgtct gccagactgg ccgccgtagc cgccgcgccg cggccgtcct gcgcgacctg
      361 ggcttcaacg acctgacgct gctggagggt gggatgcagg cctgggaagc ggccgggtta
      421 ttgaccgctg tggagtttac ctgaaatgga tactttcctg acttcgcgca actccggcct
      481 ggacctggtg cgggtgacgg aagccgccgc cctggccgct ggccgctgga tcggctccgg
      541 caacttcgcc gccgcgcacg ccgcggccac ggacgccatg ttcaacatgc tggccgcgct
      601 gcagatcgat ggccacgtgg ccattggcga agatcgcctc atcgacggcg cggccccgct
      661 ggccgaggga gccaccttcg gcgcgggcct catgccctac gacttggccg ttgatccgat
      721 cgacggcacg cgcctgctgg tggagggcaa gccgggggcc atctccgtgg tggccatggc
      781 cccgcgtcac agcatccgtt ccattgctcc ggccgagtac ctggagaaga tcgtcgtcga
      841 ccgcgacgcc gccgctgttc tggtgcccga atgtctcaac gccccggccg cctggacgct
      901 ggcgctcatc gcccggacga agaagaaagc cgtccgcgac ctggtggtcg tcgtcctgca
      961 acgcgctcgc caccacgacc tcatcgagga gatacgagcc acaggcgcgc gcgttctgct
     1021 gcgcgaggaa ggcgacgccg agggcgcgct ggtagcggcc acgccgggca gtcgcgtcga
     1081 tgtgctcatg ggcgttggcg gcgcggccca gggcggcttg gcggcctgtg ccgtgcgagc
     1141 gctacacggg gccatgctgg cccaggtcgc gccgcagtca gccgaggagc gcgccgcggt
     1201 cgatcaggcc gggttgggca accaccgcgt gctgacggcc gaggagttgg tggccagcga
     1261 cgacgtgctc ttcgccgcca cgggcatcac cgacacgtcg ctgctgcccg gcatcgccta
     1321 ccgcggcagc cacgccgaaa cccactcgct gctcctgcgg gccaagacgc gcacgcgccg
     1381 tttcatccag gccgagcata acctgagcga gtaagaggac ggcgttgcgc cgcccatagc
     1441 tgtcgccgtt gtgggctggg tgaagcttaa ttgcggatgc ccagttcgta ggcggcgcgc
     1501 gtctcgcggt ggaacagcca cgccagaccg aggatgggct ggatgagcgg gaagaagccg
     1561 tagtcggccc caaaatgcgc ccagacggtg cgaccgatgg tgtcgggcat gatatagctg
     1621 agcgtgccca ccagcagaac catggccagt tcaaagacca acatactgac tgacaaccgc
     1681 cacgcccact tccggcggac aatgaagccg acggtggcca ggagatagca caaggcggcc
     1741 acgccgctga gcgccggcgg caggaaattg gtcacgccct ccttcagaag gagttggtag
     1801 agcgcccgcg cgccggtgga aagggctaga actgggtaag agatggccag gatgacgccc
     1861 gcggcgcgga tgaagccgga cagggcattc tggctgacat cggtttgttg ggtagccatg
     1921 ccgtcgattc taccatgatc ggtgaagact tgctgaaacc gaaccggtaa cgacggcacg
     1981 agatgttaca attcggccat gtacgacgac gatcaccccg ataccccgcc tctccgaaat
     2041 gaccttaccg atctgccaga aggggagggc ggccggcgcg gctgtatctt cctgggcgtg
     2101 acgatgctgc ttatcgtcag tctcattggc tcatccctgc tggcctggtt cgtgctcgtg
     2161 cggcgagagc agacctcggc cccccctacg cctctggttg cgtccacgct tgcaccggct
     2221 acgctcgtgg ccgccctgcc gacgccacag ccgatcgccg cccagccgac ggccgcgccc
     2281 attgatggca cggccatcaa ccgtatcgtc atcgttaacc ccgaaggcca ggttgagacg
     2341 ctctcgccta cgggcggcga ccgccgcgtc ctgacgcgcg aggaggatag cgttctcttc
     2401 cagttcccgg cctggtcacc cgacggtcgc catctggccg tgattggcag ccggctcaca
     2461 ggcggcggcg tctacgtgct gccggacact cccggcgggt cggacgtttc tgccagccag
     2521 gtttacttca gcgccaacga gacgcccatc tatctcttct ggtcgcccga cagcgccacg
     2581 ctggccttcc tggccaatca gtcgcgcagc ggcatggggc taatggtcat cgacagtgcc
     2641 ggcgcgagcg aaagccggct gttggccacg ggcgcgccct tctactggga ctggagcgaa
     2701 gacggccggc aactgctcat ccacaccggc gcggaccgcg gcggcgacgt gtcacttatt
     2761 gacgtcgagg ggcaagtgca ggcggccaat gtggccacgc cgggccagtt ccaggctccg
     2821 ggtatcgctc ccggcggccg ctactgggcc tttgccgagg aaaccgatgg ccagagcgcg
     2881 cttgtcattg tcgatacgca gaccggcgag cggcgcagtt ttgagccgtc ccggtcgctg
     2941 gctctcggct ggagtcccac cggcgagcaa ctggccttca ccaatgcgac cgccgccaat
     3001 caccccttct gggggccgct gcgcgtgctg gacgcggcca ctggggaagc gcgcctgctg
     3061 acccgccaga cggtgctggc cttcttttgg tcgcccgacg gccacgccat tgccttcatc
     3121 accctcaatg acagcttcaa cgacgacggc gttaatgccc tggccaagac gcgcaccctc
     3181 gctcgcctgg ccagtcctgc ccagcagacg gcgggcttcc tgaccctttc cgtggtggac
     3241 gtgaccacag gccaggggtt gcgcctgctc gactttgaac cgacagttgt cttcctgacc
     3301 cagttcctgc ccttcttcga ccagtatgct ctaagccatc gtctctggtc gcccgacagt
     3361 cgttcgctgg tcctgccggt gcgcgaggag gagggtgacg tcatcctcat tgtgcccgcg
     3421 gccggtggtc cgccgtcctt cttggccgag ggccaggccg ccttctggag ccagcgctaa
     3481 tcgtcgactg tggccccgaa gacgccgtcg ctgacctgca cgcggagggc cgcgcccggt
     3541 tgcgcctgga tgacggagcg gatgagttgc ccgtccggcc cgcgcacgat ggcgaagccg
     3601 cgggccagcg tcgcctgtgg gctgaacgcg gccagactgc tgccggccac ggccagccag
     3661 ccgcgccgcc gctccagccc ccgcgcggca ttgaccgcca gacgctcggc caacatgtcc
     3721 acgcgctgcc ggtcgctatc gaggatgcgc tgcgggctga gcagggtcag ccattgagcg
     3781 cgggcgcgta gggcctggcg gcggccgacc agagcgctgc cgatggctgc cgtcagcgtc
     3841 gcgcgcatct cggccagcac ggggccggcg ccggacagat cgggcacggc cagttccgcg
     3901 gcggccgagg gcgtcggcgc gcgcaggtcg gcgacgaagt cggcaatggt gaagtccgtc
     3961 tcgtggccca caccgctgat aattgggtgg cgcgcggcgt agatggcgcg ggcgacgcgc
     4021 tcgtcgttga aggcccacag gtcttcgatg gcgccgccac cgcgggccag caagatcacg
     4081 tcgatgtcgt cgcgttcatc cagccgctgt agcgcgcgga cgatctgtgc cgcggccagc
     4141 tcgccttgca ccagcgtcgg ggcgatcagg acagatacga ggggattacg gcgggccagg
     4201 acaatgagga tgtcgcgcag agccgcggcg tcagccgagg tgacgatgcc gatccggcgt
     4261 gggaagggcg ggatgggctg cttgcgctcc ggatcgaaca atccctcggc ggccagcatg
     4321 tctttcagtc gctcgaaggc cgcggccaga tcgccccggc cggcgggctg catatgttcc
     4381 acgtagagtt gataaacgcc gctgacctcg tagacggaca cgcggccgtg ggcaatgaca
     4441 gcatccccct cggctgggcg gaagcgcaac cgctcggccg tcgaccgcca catgacgcac
     4501 ttcagctgcg cgccggcgtc tttcaacgtg aaatagacat ggcccgacgc cgcgcgcgtg
     4561 aagttggaaa tctcgccgct gacgtcgatg tcgcgcagcc ggtagtccac atcgaacatc
     4621 tgccgcacgt agtgcgttag ctcgccgacc gtccagatag tatggtcgag agtcgattgg
     4681 ctggcaaaag aaaacatgcc tgacctcgtg actcgcccct agccgctgac cggtcttcgg
     4741 gctacaatta catggttatc tatctcgtac aaggaaggtg ctttctggaa ggtctggaac
     4801 tgactcaatt acttgtcgat gccctcctgg acaagaaggg tgtcgatatc ctcgtcctcg
     4861 atattcacgc ccaggcgatc ttcgccgact tcttcgtgat ttgcagcggc gaatcggagc
     4921 cgcaactgcg agccttatcc caggcggcgc tggagagcgc caagaaagag ggcggccggt
     4981 cgccaaaggg cgtggaaggg acgcccgccg acggttgggt actggtcgat tttggcgacg
     5041 tcgtcgtcca cctgttctcg ccccagaagc gcagctacta cgatctggaa tcgctctggc
     5101 ataacggccg cgtcgttgtg cgcatgcagt gacccacgcg cgctcggccg gtggcccagc
     5161 gaccaccgcg ccgagcacgc ccttgcccac ggcctactcg cgaatccagc gatcggttgc
     5221 ccgctcccag ctgaccagtt cgccctcgcc aaagaagagg gccacctcgc gggcggcgct
     5281 ctccgcgccg tccgacccat gcaccaggtt gcgcccaatt tccacggcca agtcgccgcg
     5341 aatcgtgccc ggctcggctt cgcccgcctt ggtcgcgccc atcgtcttgc gaacgatggc
     5401 gatggcgtcc ttgccctccc agaccatggc gacaatcggc ccagaggtaa tgtaatcgac
     5461 caggccgttg tagaacgact tgccttcatg ggcggcgtag tgggccgcgg ccagttcccg
     5521 cgtcatttgc atcagcttca ggccgagcaa cttcagcccg cgccgctcca gccgcgtgat
     5581 gatttcgcca atcaacccgc gctggacgcc gtcgggcttc accaaaacaa gagtctgctc
     5641 cattgaaagt tcactccgat gatcatggcc gcgccttagc gcagcggttt tagcaggtct
     5701 acccaggagg ccggcctggc aggtggggca ggcggctcct ccgatttcgg cgccgccgcg
     5761 gcctcgctct cttctaccgt ggcctgctcc tgggcaacga tgccggtcgc cgcctcaacg
     5821 gtcacggttg tcacgtcggc tactgagacc tcggccgctt ctggcgctgc cggggttgtc
     5881 tcttcccgcg cggcctcagc cgccgttgcc tcaacgaccg gcgtcgcccg acggccacgc
     5941 cgccgcgggc gggcagcgac gcgcggccgc gcgtcggccg gcagcgtggc gacggtaacc
     6001 ccttccgcgg gcgcgctctc aacagccgct tccgccggcg ctttgtcgcc cggcgttgcc
     6061 tctgttaggc ctgcctcagt atccacatct ggccacggcc gggcctcttc ggccccactc
     6121 tcgagcacgg gcgcttccag tgctgccggc gcgcccagat cggcggctgt ttcgggcgtg
     6181 gctggttcct ctgccagtat ggctgctggc gacgcggacg gctctggcgg gttggccgcg
     6241 ctgttgccgg ccagcccttc cagccaggca atgatcgcgt cggggtcttc gggcatgttg
     6301 agcaggtcgg cttccgacag ctcttcaagc accggttcgg cgttgacgcc ggcggatggg
     6361 gccaaattcg cgacggccgc cgcgggcgct gcttcctcaa ccggcagcga cggcgatgag
     6421 tcaacagtgt cttcctcggc catctgctcc agccaggcca gtaccgcgtc ggggtcctct
     6481 ggcatgcggg cgatcaggtc ctcgtcgctc aacggcgccg ccggcagcgg cccggagaga
     6541 cccagggaca actccaacca ttcgaccgcc gcgggaagat cgcggcgcgc agataggcca
     6601 ccgctctggc gcagtgacga atcgatctcg gccagcgatg cgcccggcgg tagggccagc
     6661 gcgtccagag ccaacagcgt ttcttccagg ctcgcgccat cgtcgccgtt ccggcgcgcc
     6721 atcgtcgccg gcatggccat atccgcaggc agttcgcccg ccggctcatc ctcggcaggt
     6781 gatagcggtt gcctgtcgat gtccagctcg atccaggggg cttcttcggg caacatcgct
     6841 tccgcttggg tggccgcggc cggctcgacc atctccgcgg gccatgcttc ctgctcggcc
     6901 ggctcagccg gttctgtcag ttcggccagt tctatcggct cggccagttc cacgctgtcc
     6961 tgagccaggg cttctgcgtc ggtggccgcg gtttcggtcg ccggctcggt cagccattct
     7021 ggctgcgccc attctttgtc ggccctatcc acgacctgct ccagggctag ttcgccggcc
     7081 gccgggccgg ctgtcatctc gactggctca atcggctgag tggtcgatcc tggcggttcc
     7141 tgagcggcgg ccagttcgtc cagaaaggcc atcgcgccct caaaggagtc ttcgttggtc
     7201 gcgggcgcgg cgagatcggt cgcctgcggc ggcgctggcg acacagttgc cggcggtggg
     7261 ggttcttcca gcgtgcgccc ctggtcggca gccatggtct ccatcaggac gatagtatcg
     7321 gcaaaatcct cggcggctac gaacgcattg atcggtgtgt tgccttccag cagcgaaaga
     7381 tcgcccagcg acatttcatt ggttgaccca cgcagatcga gcgactcggg cgaaatgccc
     7441 gcctctcgta gcagcttcga cgccagggcg cggttggcca cgcgggcgaa gaccggggcg
     7501 gccacctcgc cgccgc
//
LOCUS       DAOSMQ010000097         7479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9884_length_7479_cov_4.562769, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000097 DAOSMQ010000000
VERSION     DAOSMQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9884_length_7479_cov_4.562769"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK829_04710"
     CDS             <1..203
                     /locus_tag="PK829_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40547.1"
                     /translation="TYYPTFKFVKAENSGNVALTGLDPNVTYAVSIAALNDRGLLSGF
                     SIEQFVGPDAASYNSGALSSVP"
     gene            complement(209..1828)
                     /locus_tag="PK829_04715"
     CDS             complement(209..1828)
                     /locus_tag="PK829_04715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU40548.1"
                     /translation="MKAKALLLATLTAVLLLLAACGAPAAVAPTIVAATPSPTGAPPT
                     TTPEPTATPEPTATATLPPTATLPPTATLPPTAQPLTLALPPQWVDLVAPVAADTSAG
                     YALTPLAVDDPAAALAAGEAQLALQRGGDGIVVRQEPLAFAVPFTTNWELLNAAEAEA
                     ILANGHNLVRVIPWSAMTPDNKALRVDGLHPTDADYPYHETWSLVAAPGQEAAAAALA
                     PRLSAALAPLPTIRLAAVGDVNIDRTQREIILSTGDYVYPFARVKDIFAAADYTIANL
                     ETSLGDVGEPVAKRYTFRSPPEAAQSLALGGVDLVSLANNHAGDYGWETLLQGIDLLH
                     AAGVATVGGGANDAAAHAPHIADIDGLKVAFLGYVHVPVEAITHFDTQTWTATADTPG
                     LAWADPERIRADVRAVRPQVDLVVVILHSGYEYIEEPSEEQVAAAHAAVDAGADLVVG
                     HHAHILQGIHRYGDGVIAYGLGNFVFNIEGPPETAILNVWLDRDGVRQLELIPAIVQQ
                     YGQPRLADFAESGPILSRVYYLTTILNAATD"
     gene            complement(1825..2775)
                     /locus_tag="PK829_04720"
     CDS             complement(1825..2775)
                     /locus_tag="PK829_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOU40549.1"
                     /translation="MRSAVRLLPVVLLCLLAAALAACRPAAVAPVAALPTPAASATAR
                     PPVVVTTAPTTASTPPSTPPSTAASTAASTPPTATTTATTAPQPTIPPTTVPSPTPNR
                     DIPCNERFADDGLLTVVTLTYGLSRDYVPRGLVPLDAYFDSDVTLGYPTELRHLVIGP
                     LAAMIADMRAAGLHPFIISGYRSYSAQAIARQKWLEQEPERANILSAPPGFSEHQLGT
                     VVDFGSPELPEIVGQEDIEFHTYFYKTSEGFWLADNAHRYGFTLSYPFEATEITGFFY
                     EPWHYRYVGVEMATQLYNDHISLTERQLATEPPPCIPDTP"
     gene            complement(2780..3631)
                     /locus_tag="PK829_04725"
     CDS             complement(2780..3631)
                     /locus_tag="PK829_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40550.1"
                     /translation="MLLYRWSSLLRAYDLRLGYNRVIVLLSALGAVVALVAGIIAGLP
                     MSDVLLRAFAGGVTAFGAAGLAKELDPDHPGSAVAGAAMAIPLAWWVARPESLLPLLW
                     LILILRFVSRSTGLPPKLTDALGLILLAGWLSWARTPLFGILTGVVLVLDVLLPHGRR
                     VFGWLGAPVALAAGLYWALNAARYPSAQAETWLVVALLALTIAIIGVILTCYVILTPG
                     DATGRPLEPVRVQAGQIVALTVGLFFASWHGDDGATLFVALWLALAGTVVLYWRDIDS
                     RRATVPS"
     gene            complement(3635..4735)
                     /locus_tag="PK829_04730"
     CDS             complement(3635..4735)
                     /locus_tag="PK829_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU40551.1"
                     /translation="MTKESLGYVELEWVCPFCNTRNPGRLKVCANCGAPQPKDVQFEQ
                     AAEDQLVKDAEGLEMAKAGPDIHCPFCGTRNPAGAKKCSRCGGDLTEAERREAGQVVG
                     AAQTKPAPDVKCPFCGTPNKATNVKCTNCGSPLAKEARPKPTVAPVMATPAATSARMS
                     PMLLLIGAAVLLLICGAVAFLVMRGTRTETSLARVTDTEWQRSIVVLGYAPASHSDWA
                     DQIPAGADVGSCTERERGRSAFPTGNSEQVCGTPYVVDQGTGFGEMVQDCEYIVYDDY
                     CQYSVVELQPVGVLRESGSDLNPFWPQTRLDSDQQLGDREETYEITFDVDGEQVTYET
                     ADANEYARFQRGSQWALEINGFGNITDIQPAP"
     gene            complement(4774..6519)
                     /locus_tag="PK829_04735"
     CDS             complement(4774..6519)
                     /locus_tag="PK829_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HOU40552.1"
                     /translation="MDAATLTIFNHLFASVAEEMGVTLNRAAYSPNIKERLDYSCAVF
                     LGGERPALLAQAAHIPVHLGAMPASVRAAVAACAPLRPGDVVILNDPYQGGNHLPDIT
                     LVSPVFIDGAGEQGSGAAGEVASPDIASFPPAPLPPRSPAFFVASRAHHADVGGMSPG
                     SMPLSTELYQEGIIIPPLKLVDGGRRNDAVWELILRNVRTPWERDGDLAAQLAAHATG
                     AARLAEIAGRYGLDETLRQARALLAYAESLTRAAIARIPDGIYRFEDALDDDGQGDSR
                     RPDVPVPIRVSVDVRGEALTVDFAGTAPAVAGNLNAVPAIVESAVVYCLRCVALALLG
                     ELPMNQGVFAPLTVRIPRGSVLDPRPPHAVAAGNVETAQRVVDVVFGALAAALPDLIP
                     AASQGTMNNLTFGGNRGQVPGSREDGMTHDPVSSPLAPDPWPLAPSFAYYETIGGGAG
                     AGPAGDGASGVHVHMSNTRNTPVEALEYSYPIRVEAYALRHGSGGRGRHTGGDGLLRA
                     IRFLSPATATITTERRRRGPYGLAGGEAGAPGRNALLRGSEVVPLPGKATLVLEPGDI
                     IWLETPGGGGWGEPG"
     gene            complement(6577..7125)
                     /locus_tag="PK829_04740"
     CDS             complement(6577..7125)
                     /locus_tag="PK829_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOU40553.1"
                     /translation="MTQRDSYTVEVAGLLRHFPLFEVAPGVRIAVFNMLGDTYVVKAS
                     AAALAERLRDIPADVLLTAEAKSIPLIYEMSALMGLPYVVLRKSYKSYMGEAISAETV
                     SITTGAPQTLFLDEKDRAFITGKRVIVVDDVISTGSTLNGMEKLVLKAGGHVAQIAAI
                     FTEGDDDWSRVVALGNLPVFRD"
     gene            7327..7461
                     /locus_tag="PK829_04745"
     CDS             7327..7461
                     /locus_tag="PK829_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40554.1"
                     /translation="MKAILVQGEKDHPVLAWGDAPEPAMGPDEVLVAVRAAAVNRADL
                     "
BASE COUNT         1212 a         2339 c         2766 g         1162 t
ORIGIN      
        1 cgacctacta cccgacgttc aagttcgtca aggccgagaa ctccggcaac gtcgccctga
       61 ccggcctcga cccgaacgtg acctacgcgg tcagcatcgc cgcgctgaac gaccgcgggc
      121 tgctgagcgg cttctccatt gagcagttcg ttggcccgga cgcggccagc tacaacagcg
      181 gcgcgctctc ctctgtaccc tagggggctc agtccgtggc cgcgttcagg atcgtcgtca
      241 ggtagtagac tcgcgagagg attggccctg actcggcgaa gtcggccagg cgcggctggc
      301 catattgctg gacgatagcc gggatgagtt ccaactgccg cacgccgtcg cggtcgagcc
      361 agacgttgag aatggccgtc tccggtggcc cctcaatgtt gaaaacgaag ttccccagcc
      421 cataggcgat caccccgtcg ccatagcgat ggatgccctg caggatgtgg gcgtggtggc
      481 ccaccaccag gtcggccccg gcgtccacgg cggcatgggc cgcggccacc tgttcctcgc
      541 tcggctcctc gatgtattca tagccgctgt gaaggatgac gacgacgaga tcgacctgcg
      601 ggcgcacggc gcggacgtcg gcgcggatgc gttcggggtc ggcccaggcc aggccgggtg
      661 tgtcggccgt ggctgtccac gtctgcgtgt cgaagtgggt gatggcctcg acgggcacgt
      721 ggacgtagcc caggaaggcg acctttaggc cgtcgatgtc ggcgatgtgg ggcgcgtggg
      781 cggcggcgtc gttggccccg ccaccgaccg tggccacgcc ggcggcgtgc agcaggtcga
      841 tgccctgtag cagcgtttcc caaccgtagt cgccggcgtg gttgttggcc agtgagacga
      901 gatcgacgcc gccgagggcc agcgactggg ctgcctccgg cggcgaacgg aaggtgtagc
      961 gcttggccac cggttcgccc acgtcgccga gcgaggtttc caggttggcg atggtgtagt
     1021 cggccgcggc gaagatgtcc ttgacccggg cgaagggata aacgtagtcg cccgtgctca
     1081 ggatgatctc gcgctgggtg cggtcgatgt tgacgtcacc gacggcagcc aggcggatgg
     1141 ttggcagcgg ggccagcgcc gcgcttagcc ggggggccag ggccgcggcg gctgcttcct
     1201 gccccggcgc ggccaccagc gaccacgtct cgtgatatgg gtagtcggca tcggtcgggt
     1261 gcaggccatc gacgcgcaga gccttattgt cgggcgtcat ggcgctccag gggatgacgc
     1321 gcaccagatt atggccgttg gccagaatgg cctcggcctc ggcggcgttg agaagctccc
     1381 agtttgtcgt gaagggcacg gcgaaggcca acggctcctg gcggacgacg atgccatcgc
     1441 cgccgcgctg cagggccagc tgggcctcgc cggcggccag ggccgcggcc gggtcatcga
     1501 ctgccagcgg cgtcagggca tagccggcgc tggtgtcggc agccaccggg gcgaccagat
     1561 cgacccactg cggcggcagg gccaacgtca gcggttgcgc cgtgggcggc aaggtcgccg
     1621 tgggcggcaa ggtcgccgtg ggcggcaagg tcgccgtggc tgtcggttcg ggcgtggccg
     1681 tcggttcagg ggttgtcgta ggcggcgcgc cggtcggcga tggcgtcgcg gccacaatcg
     1741 tgggggcaac ggccgccggc gcgccgcaag cagccagaag caacaggaca gcggtcaggg
     1801 tcgcaaggag aagggctttt gctttcaagg ggtatccggg atgcagggcg gcggctcggt
     1861 ggccagctgg cgctcggtta gcgagatgtg gtcattgtag agctgggtgg ccatctcaac
     1921 gccgacgtag cggtagtgcc acggctcata gaagaagccg gtgatctcag tggcttcaaa
     1981 cgggtagctg agcgtgaagc cgtagcggtg ggcgttgtcg gccagccaga acccctcgct
     2041 cgtcttgtag aagtaggtgt ggaactcgat gtcttcctgg ccgacgatct ccggcagttc
     2101 cggcgagccg aagtccacga ccgtgcccag ttggtgctcg ctgaagccgg gcggggcgct
     2161 gagaatgttg gcgcgctccg gctcctgttc cagccacttc tgccgggcga tagcctgggc
     2221 ggagtagctg cggtagccgg agatgatgaa cgggtgcagg ccggcggccc gcatgtcggc
     2281 gatcatggcc gccaacgggc cgatgacgag gtggcgcaac tcggtcgggt agcccagagt
     2341 gacatcgctg tcgaaatagg cgtccagcgg cacgaggccg cggggcacgt agtcgcggct
     2401 aaggccgtag gtgagcgtca ccacggtcag cagaccgtcg tcggcgaagc gttcgttgca
     2461 ggggatgtcg cggttcggcg tgggcgaagg cacggtggtt gggggaatgg ttggttgggg
     2521 cgcggtcgtc gccgtggtcg tcgccgtggg tggcgtggac gcggccgtgg acgcggccgt
     2581 ggacggcggc gtggacggcg gcgtggacgc ggtcgtgggc gcggtggtta cgacaacggg
     2641 cgggcgggcg gtcgccgagg cggccggcgt gggcagggcg gcgacggggg cgacggccgc
     2701 gggccggcag gcggccaatg cggcggcgag caggcagagc agaacgacgg ggagaagccg
     2761 gacggcgctt cgcatagcgt tacgagggga cggtcgcccg ccgcgaatcg atatcgcgcc
     2821 agtagagcac caccgtgccg gccagcgcca gccacagggc gacgaacagc gttgccccgt
     2881 cgtcgccgtg ccaggaagcg aagaacagcc ctaccgtcag ggcgacgatt tgccccgcct
     2941 ggacgcgcac tggttctagc ggccggcccg tggcgtcgcc gggggtcagg atgacgtagc
     3001 aagtcagaat gacgccgatg atggcgatgg tcagggccaa tagggcgacg accagccatg
     3061 tctcggcctg cgccgacggg tagcgggcgg cattcaaggc ccaatatagc cccgcggcca
     3121 gggccacggg cgcgcccaac cagccaaaga cgcgccggcc gtggggcagc aacacgtcta
     3181 gcaccaggac gacgccggtg aggatgccga acagaggcgt gcgcgcccaa ctgagccagc
     3241 cagccaggag aatcaggccg agcgcgtcgg tgagtttcgg cggcaggccg gtcgaacggc
     3301 tgacgaagcg caagatgagg atgagccaca gcagcggcag caggctttcc gggcgggcga
     3361 cccaccacgc cagcggtatg gccatcgccg cgccggcaac agcgctgccg gggtgatccg
     3421 ggtcaagctc cttggccagg cccgctgcgc caaaggccgt cacgccgccg gcgaaggcgc
     3481 gcaacaacac gtcggacatg ggcagaccgg cgataatgcc ggcaaccagg gccacaaccg
     3541 cgccgagggc cgatagcagg acgatgaccc ggttatagcc gagcctcaag tcataggctc
     3601 gcagcagcga cgaccagcga tagaggagca tggttcaggg cgcgggctgg atgtcagtga
     3661 tgttgccgaa gccattgatc tccagcgccc actggctgcc gcgctggaac cgggcatact
     3721 cgttggcgtc ggccgtctcg taggtgacct gctcgccgtc cacgtcgaag gtgatctcgt
     3781 aggtctcctc ccggtcgccg agctgctggt cactgtccag ccgcgtctgg ggccagaagg
     3841 ggttgaggtc ggagccgctc tcgcgcagga cgccgacggg ctgcaactcg acgacgctgt
     3901 actggcagta gtcatcatag acaatgtact cgcaatcctg caccatttcg ccgaagcctg
     3961 tgccctggtc cacgacgtaa ggcgtgccgc agacttgctc ggagttgcca gtggggaagg
     4021 cggaacggcc gcgttcgcgc tcggtgcagc tacccacgtc ggccccggcc ggaatctggt
     4081 cggcccaatc gctatgactg gccggggcat agccgaggac gacaatgctg cgctgccact
     4141 cggtgtcggt gacgcgggcg aggctggttt ccgtgcgcgt gccgcgcatg acaaggaaag
     4201 cgacggcccc gcaaatgagc agcagcacgg ctgcgccgat gagcagcagc atgggactca
     4261 tcctggccga ggtagctgct ggcgtggcca tgaccggagc gacggttggc ttcggccgcg
     4321 cctctttggc cagcggcgag ccgcagttgg tgcatttgac gttggtggct ttgttgggcg
     4381 tgccgcagaa tgggcatttg acgtccgggg cgggcttggt ctgcgccgcg ccgacaacct
     4441 gccccgcctc gcggcgctcg gcctcggtca ggtcgccgcc gcagcgggag cacttcttgg
     4501 ctccggcggg gttgcgcgtg ccgcagaagg gacaatggat gtccggcccc gctttggcca
     4561 tctccagccc ttcagcgtcc ttgacgagtt ggtcttcggc ggcctgctcg aactggacat
     4621 ccttgggctg gggcgcgcca cagttagcgc agaccttcag ccggcccggg ttgcgtgtat
     4681 tacagaatgg acagacccat tccagctcga cgtagcccag ggactccttt gtcatatcct
     4741 ctttctatga cctccacgcg ggagtttacc acatcagccc ggctctcccc accccccgcc
     4801 gccgggtgtc tccagccaga tgatatcgcc cggttccagg acgagggtgg ccttgcctgg
     4861 caagggaacg acctcgctgc cgcgcagcag ggcattgcgg ccgggcgcgc ccgcctcgcc
     4921 gccggccagg ccatacggcc cgcgccgccg ccgctcggtg gtgatggttg ccgtggccgg
     4981 cgacaggaag cggatggccc gcagcaggcc gtcgccgccg gtgtggcggc cgcgcccgcc
     5041 agagccgtgc cgcagcgcat acgcctcgac gcgaatgggg tagctatact ccagcgcctc
     5101 gaccggcgtg ttgcgcgtgt tgctcatgtg gacgtggacg cccgacgcgc cgtcgccggc
     5161 cggcccggcc cccgcgccgc cgccgatggt ttcgtagtag gcgaaagaag gggccagggg
     5221 ccaggggtca ggggccagag gagacgagac aggatcgtgg gtcatgccat cttccctgga
     5281 acctggaacc tggcccctgt ttcctccaaa cgtcaggttg ttcatcgtcc cctgcgacgc
     5341 cgccgggatg aggtcgggca gggccgcggc cagcgcgccg aagacgacat cgacgacgcg
     5401 ctgggccgtt tcgacgttgc cggcggcgac ggcatggggc gggcgcgggt cgagcacaga
     5461 gccgcgcgga atgcgcaccg tcagcggggc aaagacgccc tggttcatgg gcaactcgcc
     5521 cagcagcgcc agggcgacgc agcgcaggca gtagaccaca gccgactcga cgatggctgg
     5581 cacggcgttg aggttgccgg ccacggccgg ggccgtgccg gcgaaatcga ccgtcagcgc
     5641 ctcgccgcgc acgtccacgg aaacacggat gggcacgggc acatcggggc gacgggagtc
     5701 gccttggccg tcgtcgtcga gcgcgtcctc gaagcggtag atgccgtcgg ggatgcgggc
     5761 gatggccgcg cgggtcaggc tctcggcgta ggccagcagg gcgcgggcct ggcgcagtgt
     5821 ctcgtcgagg ccgtagcggc cggcgatctc cgccagccgg gccgcgccgg tggcgtgggc
     5881 ggccaactgg gccgccaggt cgccgtcgcg ctcccacggc gtgcgcacgt tgcgcaggat
     5941 cagctcccag acggcgtcgt tgcgccggcc gccgtctacc agcttcagcg gcggaatgat
     6001 gatgccttcc tggtacagct ctgtggacag cggcatcgag ccgggcgaca tgccgccgac
     6061 gtcggcgtgg tgggcgcggc tggcgacgaa gaaagcaggg gagcgggggg gcaggggggc
     6121 gggggggaaa gaagcaatgt ccggtgaagc cacctcccct gctgccccgc tcccctgctc
     6181 ccctgcgccg tctatgaaca ccggcgatac cagcgtaata tccggcagat gattgccgcc
     6241 ctggtagggg tcgttaagga tgacgacgtc gccggggcgc aggggcgcgc aggcggcgac
     6301 ggcggcacgg acggaggccg gcatggcccc caggtggacg gggatgtggg cggcctgggc
     6361 cagcagcgcc ggccgctcgc cgcccagaaa gacggcgcaa ctgtagtcga gccgctcctt
     6421 gatgttgggg ctataggcag cgcggttgag cgtgaccccc atctcctcgg ccacggaggc
     6481 gaagaggtgg ttgaagatgg tcagggtggc ggcgtccatc gtgaagaagg gggccagggg
     6541 ccaggttcca gggcgctctt tcctggcccc tggaacctaa tccctaaaca cgggcaaatt
     6601 gcccagggcc acaacgcgcg accagtcgtc gtcgccttcg gtgaagatgg cggcgatctg
     6661 ggcgacgtgg ccgcccgcct tcaggacgag cttctccatg ccgttgagcg tactgccggt
     6721 gctgatcaca tcgtcaacga cgatcacacg tttgccggtg atgaaggcgc ggtctttctc
     6781 gtccagaaag agcgtctgcg gcgcgccggt ggtgatcgac acggtctcgg cgctaatggc
     6841 ctcgcccatg tagctcttgt agctcttgcg taggacgacg tagggcaggc ccatcagggc
     6901 gctcatctcg tagataaggg gaatgctctt ggcctcggcc gtcagcagca cgtcggcggg
     6961 gatgtcgcgt agccgctcgg ccagcgccgc ggccgacgcc ttgaccacgt aggtgtcgcc
     7021 gagcatgttg aacacggcga tgcgcacacc gggggcgacc tcgaaaagcg gaaagtggcg
     7081 cagcaggccg gccacttcga ctgtgtagga atcgcgttgg gtcatggggt gctctcctga
     7141 tgaagaaatt taacgcaaag acgcgaagac gcaaggcgcg aagaaaatct cacgcaaggc
     7201 gcgaagaaaa tctcatgcaa agacgccaag aacgccaagc tcggaatctt tctgagcttg
     7261 gcgttcttgg cgtctttgcg tgagaccgtc tttgcaccct tgcgtctttg cgttaaattt
     7321 ctggccatga aagcgatcct ggtacaaggc gagaaggacc atccggtgtt ggcttggggt
     7381 gacgcgcctg agccggcgat ggggccggac gaggtgctgg tggcggtgcg cgccgcggct
     7441 gtcaaccggg ccgacctgta gcaggcgcgc ggccactac
//
LOCUS       DAOSMQ010000098         7447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9940_length_7447_cov_3.761093, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000098 DAOSMQ010000000
VERSION     DAOSMQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9940_length_7447_cov_3.761093"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1000
                     /locus_tag="PK829_04750"
     CDS             134..1000
                     /locus_tag="PK829_04750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOU40555.1"
                     /translation="MQIGLVYPQTEYPADPAAVRDYAQTAEALGFAHVLAYDHVLGAN
                     PDRPGGWSGPYTYQTSFMEPFLLFTYMAAVTTRLGFATGILILPQRETALVAKQAATL
                     DVLCGGRLRLGIGTGWNEVEYIALGQDFHTRGRRQEEQIEVLRALWTQELVTFKGRWH
                     DIPDAGLNPLPVQRPIPLWLGGHADAVLRRLARLGDGWLPGFRTAEAATGALATLDGY
                     LAEAGRSRADIGLEPRLHWGDGDLDALGRTLAGWRAAGATHVSLNTMGAGFKTAGEHL
                     AAVRQFAEKLVQ"
     gene            complement(1016..1435)
                     /locus_tag="PK829_04755"
     CDS             complement(1016..1435)
                     /locus_tag="PK829_04755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOU40556.1"
                     /translation="MSRPHIPTSLRTLVFERAAGRCEYCRIHQDDVASRHPIDHITPI
                     SHGGETDEANLALACVLYNTAKGSNLSGVDPLTGRVAALYDPRRQKWSRHFALEGAAI
                     VGLTATGRTTIRVLRLNDPQRSDERLRLQAIHRYPRR"
     gene            complement(1441..1809)
                     /locus_tag="PK829_04760"
     CDS             complement(1441..1809)
                     /locus_tag="PK829_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40557.1"
                     /translation="MATITLEVPDELAQRLESVGGRLPALLAYALDVAGIPGSSIASE
                     HSPLWGEVIEFLGSGPSAEEVLAFKISDGAQGRLEDLLYLNREETLTPQEQDELDAFA
                     QIDHLFTLLKAYLRQTNSAA"
     gene            1835..3103
                     /locus_tag="PK829_04765"
     CDS             1835..3103
                     /locus_tag="PK829_04765"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOU40558.1"
                     /translation="MELLGGPLFAVAGAGESHGPGIVTIVFGCPPGLWLERAAVQAYL
                     DRRRPGSTRHGTPRREADRVAFLAGLYQDDHDRLTAGPPLSFGDALGDSQTAATYEAG
                     ATTGEPIAAVVLSTAGRSGHYDPFRGESGVVRPGHTDLVKFHQSGGAADIRGGGRSSY
                     RATISDVIGGSVARLYLRAAFGTVLLSSVAQVGPLRAAQTLADAVRDFGPHPAPERVA
                     ALEEAVAATWTGVGIPTIDAGFAVAAADLIKHTRVAGDSLGAAVEVVAVRVPALAGDP
                     LYNSLKLRLMGSLGGLHAAQACEIGDGAAVAERRGSDNNDPIRAAGYAGNSHGGLLGG
                     VTTGNPLVARVSFKPTSSIVRPQLSVRKNLEEIDFELGQGRHDPCVGVRAGITLESRM
                     AIEVMNAVLMHAARHVDADSFRLFPPAADR"
     gene            complement(3776..4549)
                     /locus_tag="PK829_04770"
     CDS             complement(3776..4549)
                     /locus_tag="PK829_04770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40559.1"
                     /translation="MDIAEVLALYDREQRREITFPDMERHALTDIVRFVRPKPGMSFI
                     LYSDLDEATADAAIDAQIAYFANNNLKMEWKVYGHDRPADLAQRLIARGFEPEDEDAI
                     MALDVAHAPAALLAEPAADVRRLTDPAQLADVVAVLEAVWTEKFGWVYERLGGHMAIP
                     GYLSVYVAYVGERPAAAGWTYFSEGSFASLWAGSTLPAYRRRGLYTALLAARVREARA
                     RGVPYLTIDAGSMSRPVVARHGFALLTTAISCEWKGDGR"
     gene            4625..5215
                     /locus_tag="PK829_04775"
     CDS             4625..5215
                     /locus_tag="PK829_04775"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOU40560.1"
                     /translation="MDDTRYFWKGRLTRLRPVSPDDAEGTWRDRLDSPGRQVLQLGIE
                     LPTTSDDLRDRLARFANCRDIDGLILFTIENLDGEDAGGLSYHTRNRKDGNFSFGVRV
                     AAPFRGRGYAQDATRILLRYGFLERNYHKCNSACVEGNTASIRLHHALGFRDEGRRRE
                     TLFINGRYYDELLFGLTRAEFDANDAPYRPDWMGAT"
     gene            5212..6141
                     /locus_tag="PK829_04780"
     CDS             5212..6141
                     /locus_tag="PK829_04780"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOU40561.1"
                     /translation="MNTEWTADAGADAIGRVLVVGAGTMGRQIALQCALYGLDVDLYD
                     TAPDALAAAHNEINILAASFARHGRLSPAEATAAAARVAPTSDLPAAAARADLVSESV
                     PEDPALKGRVFAQLNELCPPHTVFTTNSSTLLPSMFAAATGRPARFAALHFHLPPWDA
                     NVVDIMAHSDTAPAVVELLVAFARRIGQIPIVTQRESSGYVFNAMLSALNGAALKLAA
                     EGIADVTDIDRAWMGIMKTPIGPFGIMDMIGLETIWTITDFWANALGDRGLRANADFV
                     RGYLDRGRHGRKNGHGFYDYPHPAFAADGFLEG"
     gene            6163..>7447
                     /locus_tag="PK829_04785"
     CDS             6163..>7447
                     /locus_tag="PK829_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU40562.1"
                     /translation="MSPAIDRYLNVKLAYMPSFSSDGCWLVFISDLTGVPQAWRVSLG
                     DTGRTITMPEQLTFAADRVLWLCCSPAPGDRRILFTRDRGGNENAQLWLLDPAQGVET
                     CLTEGCDDVMHIPGQWSADGHTILFAANRDHRSHFGIYRQTLDSGVVELLWQSDVPGL
                     LYDVAAHPTADRVLFTRMGFSAAHDLMELDLSTGHARALSPTDRPARYQYPVYSADGA
                     YVYVVTDFDSDRTNLLRFDLAAGLWETVVGPLYDLEHFSLSTNGRFLAFTINQDGYSR
                     MELLDMERGEAQPGPILPDGVLGIHDERIAFDPTSSSLAFAYTTPRSTSNVYLWNWAT
                     MGQSVRPLTHMTQGGIAPDSFVDAELIRYPTFDGRAIPAWLYRPRTATGPVAVVVNVH
                     GGPEWQARPWFDFLTQYLVACGYAVLVPNVRGSTGY"
BASE COUNT         1222 a         2665 c         2289 g         1271 t
ORIGIN      
        1 gggcggcctg aagtttcctt tgcaccagag agagtttggc cgcccacctg tcagggcttc
       61 gagcaactga cgactttgga aaagccctgg actgactgac tactgactga cggaatactg
      121 ctatactctc cccatgcaaa tcggcctcgt ctacccccaa accgaatacc ccgccgaccc
      181 cgccgccgtc cgcgactacg cccagacggc cgaggcgctc ggcttcgccc acgttctggc
      241 ctatgaccac gtcctcggcg cgaaccccga ccggcccggc ggctggagcg gcccctacac
      301 ctaccagacg tcattcatgg agccgttcct gctattcacc tacatggccg ccgtcaccac
      361 ccgcctcggc ttcgccacag gcattctcat cctgccccag cgcgagacag ccctggtggc
      421 caagcaggcg gcgacgctgg acgtgctctg cggcggccgg ctgcgcctgg gcatcggcac
      481 gggctggaac gaggtcgagt acatcgccct gggccaggac ttccacaccc gcggccggcg
      541 gcaggaggag caaatcgaag tgctgcgcgc cctgtggacg caggaactgg tcaccttcaa
      601 gggtcgctgg cacgacatcc ccgacgccgg cctcaacccc ctgccggtgc agcggcccat
      661 cccgctctgg ctcggcggcc acgccgacgc cgtgctgcgc cggctggccc ggctgggcga
      721 cggctggctg cccggcttcc gcacggccga ggccgccacc ggcgccctgg cgacgctcga
      781 cggttatctg gccgaagccg gccgcagccg cgccgatatc ggcctggagc cgcggctgca
      841 ctggggcgac ggcgacctgg acgcgctcgg ccggacgctg gcgggctggc gcgctgccgg
      901 ggcgacgcac gtcagtttga atacgatggg ggcggggttc aagacggcgg gggagcacct
      961 ggcggccgtg cggcagttcg ccgagaagct cgtgcagtga gcgccgcagc gccattcacc
     1021 gccgcgggta ccgatggatg gcctgaagcc ggagccgttc atcgctccgc tgcggatcgt
     1081 tcagccgcaa gacccggatc gtcgtccggc ctgttgccgt tagcccgaca atagcggccc
     1141 cctcaagggc aaagtggcgg ctccacttct ggcgacgcgg atcgtacagc gcggccactc
     1201 gtccagtcag gggatcaacg cccgaaaggt tgctcccttt ggccgtattg tagagtacac
     1261 aagccagggc caggttggcc tcgtctgtct cgccaccatg tgatattggc gtaatgtggt
     1321 caatcggatg gcgcgacgcc acgtcatcct gatggatgcg acagtactca cagcgaccgg
     1381 cggcccgctc gaagacaagc gttcgcagcg atgtcgggat atgcggtcga gacacaagtt
     1441 tcaggcagcc gagttggtct ggcgcaaata cgccttgagc agggtgaaga gatggtcgat
     1501 ctgggcaaag gcatccaact cgtcttgttc ctgcggggtc agagtttctt cgcggttcaa
     1561 atagagaagg tcttctaaac gcccttgagc accatcggag atcttaaagg ctaggacttc
     1621 ctcggcgctg ggaccgctac ccagaaactc gataacctcg ccccagagag gggagtgttc
     1681 ggaggcaatt gaactgcccg gtataccagc cacatcgagc gcgtaagcca gtagcgccgg
     1741 caatcgcccg cctacactct ccaggcgttg cgccaactca tctggaacct caagcgtaat
     1801 tgttgccatg cgcgcattat agcataattg cctcatggaa ctcctcggcg gccctctctt
     1861 cgccgtggcc ggcgcgggcg aatcgcacgg ccccggcatc gtcaccatcg tcttcggctg
     1921 cccgcccggc ctgtggctgg agcgcgcggc ggtgcaggcc tacctcgacc gccgccgccc
     1981 cggcagcacc cgccacggca cgccccgccg cgaggccgac cgcgtcgcct tcctggccgg
     2041 cctctaccag gacgaccacg acaggctgac cgccggcccg ccactgtcgt tcggcgacgc
     2101 gcttggcgac agccagacgg cggccaccta cgaagccggc gcgaccaccg gcgaacccat
     2161 cgccgccgtc gtcctgtcca ccgccggccg ctccggccac tacgacccct tccgcggcga
     2221 gagcggcgtc gtccgccccg gccacaccga cctggtcaag ttccaccagt ccggcggcgc
     2281 ggccgacatt cgcggcggcg gccgctccag ctaccgggcg accatctccg acgtgatcgg
     2341 cggcagcgtc gcccggctct acctgcgcgc ggccttcggc acggtcttgc tgtcgtccgt
     2401 ggcccaggtc gggccgttgc gcgctgcgca aacgctggcc gatgccgtgc gcgactttgg
     2461 cccccacccc gcgccggagc gcgtcgccgc gctggaggaa gctgtcgcgg cgacatggac
     2521 cggtgtgggc atccccacca tcgacgccgg cttcgccgtc gccgcggccg acctgatcaa
     2581 gcacacccgc gtggccggcg attcgctggg cgcggccgtg gaggtcgtgg ccgtgcgcgt
     2641 gcccgcgttg gccggcgacc cgctctacaa cagtctcaag ctgcggctga tgggcagcct
     2701 cggcgggctg cacgcggccc aggcgtgcga gatcggcgat ggcgcggccg tggccgagcg
     2761 gcgcggcagc gacaacaacg accccattcg cgccgcgggc tacgccggca acagccacgg
     2821 cggtctgctg ggcggcgtca cgaccggcaa tcccctcgtc gcccgcgtca gcttcaagcc
     2881 aactagttcc atcgtccggc cacagttgtc ggtgcgcaag aacctggaag agatcgactt
     2941 cgagctgggc cagggccgcc acgacccctg cgtcggcgtg cgcgccggca tcactctgga
     3001 gtcgcgcatg gccatcgagg tcatgaacgc cgtcctcatg cacgccgccc gccacgtcga
     3061 tgccgacagc ttccgtctgt tcccgccggc ggccgaccgc tgaccgccga ccgctgtgtc
     3121 gcctgccggc cgacgactgt ccactgtcta ctgcttactg acctactgcc tcctatcccc
     3181 taacccctcc cctgtctact ctgtccgttg agcgtttgtt aaatatggca caaatgctta
     3241 gttacactca ggccacagtc tgctatactg tcatcttgta cgttgctcta tgggccggac
     3301 agtccgtcat ctgcccaaca tagtacccac tggatacctg ccgccctggc tgtgtccgca
     3361 atgatggatg accaaaacgc ccgcgccacc tgcatggtgc gtggccctgt ctaattcttc
     3421 cagagcaagc gggggtgata tgatgaacct gtcactatat gtcgaatcgc gccggacgct
     3481 ggccaagctg ttggccgaag ccgggccgtg cccgttgggc aaaggcagct ggctatccta
     3541 ccccgaggat atctttgagg atctgcgcca agaagttgcc gcggcgattc agtccgagtg
     3601 tacacgcgaa gatattcccc cctggcaacc cgcgccacgg gtagcactga gctactagaa
     3661 acaacaagac gcggcccacg ccgcgtcttt ttgtttctgc cagtgcgtgg ccccttccga
     3721 agggcatcgc acttttcttg tggttggcgc aagaaagtgc ccggcacgaa gagccttagc
     3781 ggccgtcgcc cttccactca cacgagatgg ccgtcgtcag caacgcgaag ccgtgccgcg
     3841 ccacgacggg gcggctcatg ctgcccgcgt cgatggtcag atagggcacg ccccgcgccc
     3901 gcgcctcgcg cactcgcgcg gccagcaagg ccgtgtatag cccgcggcgg cggtaggccg
     3961 gcagcgttga cccggcccac aggctggcga aactaccttc gctgaagtac gtccagccgg
     4021 ccgccgccgg ccgctcaccg acataggcca cgtagacgct cagatagccg gggatagcca
     4081 tgtgcccgcc cagccgctca tagacccagc cgaacttctc agtccacacc gcttccagga
     4141 cggcaactac atccgccagt tgcgcggggt cggtcagccg gcgcacgtcc gcggccggtt
     4201 cggccaggag cgctgccggc gcgtgggcca cgtcgagggc cataatggcg tcctcgtcct
     4261 ccggctcaaa gccgcgggca atgagccgct gcgccaggtc ggccggccgg tcgtggccat
     4321 agaccttcca ctccatcttc aggttgttgt tcgcgaagta agcgatctgc gcgtcgatgg
     4381 ccgcgtcggc cgtggcctcg tccaaatcgc tatagagaat aaaactcatg cccggtttcg
     4441 gccgtacaaa gcggacaatg tccgtcagcg cgtgccgctc catatctgga aaagtaatct
     4501 cgcggcgctg ttcgcgatca taaagcgcca gcacctcggc aatatccata agcgtgctcc
     4561 ttatttgtag tcccggccgg caaatgcgat aattctagcc gacgacccgc gcgagaactg
     4621 tattatggac gacactcgct acttctggaa aggacgcctc acccggctgc ggccggtgtc
     4681 gcccgatgac gccgagggga cctggcgcga ccggctggat agccccggcc ggcaggtgtt
     4741 gcaattgggc atcgaactgc ccactactag cgacgacctc cgcgacaggc tggcccgctt
     4801 cgccaactgc cgcgacatcg acggcctgat ccttttcacc attgagaacc tcgacggtga
     4861 ggacgccggc gggctatctt accatacgcg caaccgcaag gacggcaact tcagcttcgg
     4921 ggtgcgcgtc gccgcgccgt tccgcgggcg cggctatgcc caggacgcca cgcgcatcct
     4981 gctgcgctat ggcttcctgg agcgcaacta ccacaaatgc aactccgcct gtgtcgaggg
     5041 caacaccgcc tccattcgcc tgcaccatgc cctcggcttt cgcgacgaag gccggcggcg
     5101 cgagacgctc ttcatcaacg ggcgctacta tgacgagttg ctctttggtc tgacgcgcgc
     5161 ggagttcgac gccaacgacg ccccctatcg gcctgactgg atgggagcta catgaacact
     5221 gaatggactg ccgacgccgg cgctgacgcc attggccgcg tactcgttgt gggcgcgggc
     5281 acgatggggc gacagatcgc cctgcaatgt gccctgtacg gcctcgacgt tgacctgtat
     5341 gacaccgcgc cagacgctct ggctgccgcc cacaatgaga tcaacatcct ggcggcctct
     5401 tttgcccgcc acggccgcct ctcgccggcc gaggcgacgg ccgcggcggc ccgcgtggcc
     5461 ccaacgagcg acctgcccgc tgccgcggcc cgcgccgacc tggtttcgga gtccgtgccc
     5521 gaagacccgg cgctgaaggg tcgcgttttt gcccaactca acgaactctg cccgccccac
     5581 accgtcttca ccaccaactc ctccaccttg ctgccgtcaa tgttcgccgc ggccaccggc
     5641 cggccggcgc gcttcgccgc gctgcacttc cacctgccgc cctgggacgc caatgtcgtt
     5701 gacatcatgg cccacagcga taccgcgccg gctgtcgtcg agctgctggt cgccttcgcc
     5761 cgccgcatcg gccagatacc catcgtcact cagcgcgaaa gctccggcta cgtctttaac
     5821 gccatgctta gcgctctcaa tggcgcggcg ttgaagctgg ctgccgaagg catcgccgac
     5881 gtgaccgaca tcgaccgcgc ctggatgggc atcatgaaaa cccctattgg cccgtttggc
     5941 atcatggaca tgatcggcct ggagacgatc tggacgatca ccgacttctg ggccaacgct
     6001 ctgggcgaca ggggcctgcg ggccaacgcc gacttcgtcc ggggctacct cgaccgcggc
     6061 cggcacgggc gcaagaacgg ccacggcttc tacgactatc cccatccggc cttcgccgcc
     6121 gatggctttc tggagggtta gcggacagcg cgccgcgtcg ccatgtcgcc cgccatcgat
     6181 cgctacctca acgtcaagct ggcctacatg cccagcttca gcagcgacgg ctgctggctc
     6241 gtcttcatca gcgacctgac cggcgtgccg caggcgtggc gcgtgtccct gggcgacacc
     6301 ggccgcacca tcaccatgcc ggagcaattg accttcgccg cggatcgcgt cctgtggctg
     6361 tgctgctcgc ccgcgcccgg tgataggcgc atcctcttca cccgcgaccg tggcggcaac
     6421 gagaacgccc aactctggct gctcgatccg gcgcagggcg tggagacctg cctgaccgaa
     6481 ggctgcgacg acgtcatgca catccccggc cagtggtcgg ccgacggcca caccatcctc
     6541 ttcgccgcca accgcgacca ccgcagccat tttggcattt accggcagac gcttgatagc
     6601 ggcgtggttg agttgctgtg gcaaagcgac gtgccgggtc tgctctacga cgttgccgcc
     6661 cacccgaccg ccgaccgcgt cctctttacg cgcatgggtt tctcggccgc ccacgacctg
     6721 atggagttgg atctgagcac cggccacgcc cgcgccctta gcccgacgga tcgtccggcc
     6781 cgctaccagt accctgttta tagcgccgat ggcgcttatg tctacgtcgt cacggacttc
     6841 gacagcgatc ggacgaacct gctgcgcttt gacctggcgg ctggtttgtg ggagaccgtc
     6901 gtggggccgc tctacgatct ggagcacttc agcctgtcaa ccaatggccg ctttctggcc
     6961 ttcaccatca atcaggacgg ctatagccgg atggaactgc tggacatgga gcgcggcgag
     7021 gcgcagccag ggccgatctt gcccgacggc gtgctgggta tccacgatga gcgcatcgcc
     7081 tttgacccca catcgtcctc gctggccttc gcctatacca cgccacgcag caccagcaat
     7141 gtctacctgt ggaattgggc gacgatgggc cagagtgtgc gcccgctgac ccacatgacc
     7201 cagggcggca tcgcgccgga cagctttgtc gatgccgagt tgatccgcta cccgacgttc
     7261 gacggccgcg ccattccggc ctggctctac cggccgcgca cggccaccgg gccggtcgcg
     7321 gtggtcgtca acgtccacgg cgggccggag tggcaggcgc ggccgtggtt cgacttcctg
     7381 acgcaatacc tcgtcgcctg cggctatgcc gtcctcgtgc ccaacgtgcg cggctcgacc
     7441 ggctacg
//
LOCUS       DAOSMQ010000099         7435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_9971_length_7435_cov_4.145528, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000099 DAOSMQ010000000
VERSION     DAOSMQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9971_length_7435_cov_4.145528"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK829_04790"
     CDS             complement(<1..210)
                     /locus_tag="PK829_04790"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU40563.1"
                     /translation="MNDKREITGWMMYDWANSAFSTTVVTALLGPYIQSLAESAAEPF
                     RFLGAAIEPGAIYPFAVSLSVVLQVI"
     gene            complement(335..1876)
                     /locus_tag="PK829_04795"
     CDS             complement(335..1876)
                     /locus_tag="PK829_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOU40564.1"
                     /translation="MTRKGLLRLASLLAALFTGLTLVACGGPAVAQVTPFPTGTTSPT
                     IAATDTHTPEPPVSPSPAASNTPAATAEPGSTDTPRPTATAPATTTPLPTATPTSVPQ
                     FNPTPALNITGSMREGVPTPPTAIPSPVPAFELPEGTTNILLLGKDQNADGSDARSDT
                     MIIVSVNREAQTASMISLPRDLYVYMPNRIMGRLNTAVNLGGVELLEQTILYNFGIPI
                     HYYAQVDFDGFKQILDLLGGVTMAVSCPLEDWRLISPELDPTLEENWERFTLEAGIHQ
                     MDGDLALWYARSRLTTNDFDRGRRQQQLLTAMLNQSVDLGLVAKAPELWSTFHEVVET
                     DMDIGRILQLASMAPDVRDNGVQHLYLAGKMQAWTVPETGAAVQLPIWEGENMMYDTF
                     RRLLLPPALNRADRAPITVEIVNASQYPAQGLLAADNLAWYGFVPVMGETRAPQATSE
                     MTYYGDNFKGSYDWLFAWVMGMPRDNIRLSDETSAANYRVVIGDDYNPCRPAFLAPQE
                     ALPES"
     gene            complement(1904..2815)
                     /locus_tag="PK829_04800"
     CDS             complement(1904..2815)
                     /locus_tag="PK829_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40565.1"
                     /translation="MSVNSRPESGDKPVYIYNADRRWANWREIVGDKLRDLRHYHYLL
                     RNLVVRDLKARYKNSILGILWSILNPLFLMLVFTMLFTVMANSALRQYPVFVLVGLIP
                     WNFFSGALTSGTTSVTGNSALVKKVYFPRELLPTSALLSNLVNFLFAFAVLVVFLYVF
                     GIGLTIHALWVPVLLLTQLIFTLGLCLLLGSLTVFYRDVLMILEVVILAWFFMTPVFY
                     SLEMFGPTATVLGVTFNPAQVMRWVNPMASIIDGYRTVLWGTYGSNGPVGMNAAYLVR
                     TLVTSLIVLMIGYGVFVRLNPYFGEKL"
     assembly_gap    3005..3104
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3214..4476)
                     /locus_tag="PK829_04805"
     CDS             complement(3214..4476)
                     /locus_tag="PK829_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU40566.1"
                     /translation="MRILHVVHQYPPQHVGGTEVYTRAVAGYQAAAGHAVAVFCPEPG
                     DVRPTPDDETGRLVVHRAGDGARGRTAVFLSTFGGGRLAGSFAAALAETQPDVVHLQH
                     LMGLPLALVEQIKAAGLPYVISLHDYWYGCANAQLLTNDDGRLCRGPDAGHVNCGRCA
                     LARAGLGGLTALAPAVAPLMARRGRALRQVFAGAAAVLASTGFVRRAYAGMGFPTERV
                     HIWPLGIDAGADELAAARAAQTARPAGEPLRAGYIGGLSPQKGVHHLVAAVQRLPAEA
                     AALSIYGDASAFPDYAAGLRAAATHPGIRFPGRLSRAALWPALGGLDVLVVPTLWYET
                     YSLIAHEAFAAGVPVVASRIGVMAEVVRDGVDGLLFPPADEAALADILLALAEQPERL
                     AALRAGIRPVPTLADHSRRLLGLYESLS"
     gene            complement(4552..5928)
                     /locus_tag="PK829_04810"
     CDS             complement(4552..5928)
                     /locus_tag="PK829_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU40567.1"
                     /translation="MVLVTPAYPPFPGGGERYVGAVARGLVAAGWRVTVLTSSAATEA
                     DFWQGAPATSDKDEDGVRVIRRPVRPTPGGRTGLLLWRKGMVLLSSLPTDPAGALMRM
                     ARRVPAIDGLDETLAGLDADVLHAFNGSWECGLVAAYARALATATTASPTATAASPTA
                     TAASPTATAASPTATAASPTATAASPTPLVVTPFAHLGEHGNDRVARNSTMSHQLRIM
                     RSADRLLTLTTVEADGLARYHVPAERLGVIGSAADPPPADFRASPYYATDHCEARGTF
                     GVYIGRLSYDKGALHAADAIRTLRQRGRDVALLLIGSSTPEFERYRRGLSPHDRAAIR
                     TLGVLSDRDKHAVLSRAKFLMLPSRSDSFGIVILEAWSHSVPVIAARAGGIPGVVDDG
                     ATGLLVPFADVEGLVAAAERLISDEPFAQRLGWQGYERLRSGYNWATVVKRVASHYEQ
                     VIAAHHQR"
     gene            complement(5954..>7435)
                     /locus_tag="PK829_04815"
     CDS             complement(5954..>7435)
                     /locus_tag="PK829_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOU40568.1"
                     /translation="HCPAPFISTPALGDLDGDGDLEIVIGGIDKRIYAFHHTGQRVAG
                     FPPPSALYARLGWENLHNRLADSIWSSPALADMNGDGRLDILIGSDEGNIDSSQPGDS
                     GGWTCPYRLPGGWMEGYCGGSLYGLTGGGALLPGFPQYRLEIIQSTPALADVTGDARP
                     EIFVGMGTFYYNNSPDRPTYGQRFYAFDSQGRELPGWGGGQPTGDLVPGSAAVGDIAG
                     DSAPEIVVATLQGRLFAWHADGSLVSGFPMTPRSPFGDTDRQDVGKGVILGDYDGDGK
                     MEIFMTIGWSIGIIDGNGQMLTKTSAAGRPFYYAQGLLMNNPVLADVDGDGQLELIAH
                     NSKLYVWDLPGGATRADWPMFKHDPARTGALSSAAKTATLRLSPEELEIGYAPNLTRQ
                     LRAILTLDVPATSYDWRVSSSNSNITFPRASGTATGWTQVAVDVRVPDNLGLGQHALG
                     TITVAVTGHGTTIRDNEDSVDVSVQIVRGSTRAKVPVIFAPRP"
BASE COUNT         1251 a         2347 c         2596 g         1141 t
ORIGIN      
        1 gatgacctgc aagacgaccg agagcgacac ggcaaacggg tagatggctc ccggctcgat
       61 ggccgcgccc aggaagcgga acggttcggc ggccgactcg gccaatgact gaatgtacgg
      121 ccccagcagg gcggtgacga cggtcgtact gaaagccgag ttggcccagt cgtacatcat
      181 ccagccggta atctcacgct tatcgttcaa cggtaccctc ccaggcggcc aacaagcggg
      241 acgatggtcg tcgccaagat ggccatcgtc cctgctaagg actgttgcgc gtcggcaaca
      301 gatggattat gcccatatgc gcgcgggccg gcaattacga ctccggcagc gcctcttgcg
      361 gggccaggaa ggccgggcgg caggggttat agtcgtcgcc gatgaccacg cgatagttgg
      421 cggcgctggt ctcatccgaa agccggatgt tgtcgcgggg catgcccatt acccaggcga
      481 acagccaatc gtaggaacct ttgaagttat cgccgtagta ggtcatctcg ctcgtggcct
      541 gtggcgcgcg cgtctcgccc atgaccggca caaagccata ccaggccagg ttgtcggccg
      601 ccagcaaccc ctgggcggga tactggctgg cgttgacgat ctccacagtg atgggcgcgc
      661 ggtcggcccg attgagcgcc ggcggcagca gcaggcggcg gaaggtatcg tacatcatgt
      721 tctcgccttc ccagatgggc aactgtaccg ccgcgccggt ctcaggaacc gtccaggcct
      781 gcattttgcc ggccagatag aggtgctgca cgccgttgtc gcgcacgtcg ggagccatcg
      841 acgccaactg taggatgcga ccgatgtcca tgtccgtctc gacgacttcg tggaacgtgc
      901 tccacagttc cggcgctttg gctaccagac ccagatcgac gctctgattg agcatggccg
      961 tcaggagttg ttgctggcgg cggccgcggt cgaagtcgtt ggtggtcagg cgcgagcggg
     1021 cataccacag ggccaggtcg ccgtccatct ggtggatgcc ggcttccaac gtgaaccgct
     1081 cccagttctc ctccagcgtc gggtccaact ccggcgagat gagccgccag tcttccagcg
     1141 ggcagctaac ggccatcgtt accccaccca gcagatcgag gatttgctta aagccatcaa
     1201 agtcgacctg agcgtagtag tgaatgggga tgccgaagtt gtagaggatc gtctgttcca
     1261 acaactcgac gccacccagg ttcacagccg tgttcagccg gcccatgatg cggttgggca
     1321 tgtaaacgta gaggtcgcgc ggcagcgaga tcatcgatgc cgtctgcgcc tcgcggttga
     1381 cggagacgat gatcatcgtg tccgagcgcg cgtcgctgcc gtcggcgttc tggtccttgc
     1441 ccaacaggag aatgttggtc gtgccctccg gcagctcgaa ggcaggcacg ggcgagggga
     1501 tagccgtcgg cggcgtgggc acgccctcgc gcatggagcc ggtgatgttc agcgctggcg
     1561 tggggttgaa ctggggcacg ctggtgggcg tggccgtcgg gagcggcgtg gtcgtggccg
     1621 gcgcggtggc cgtcggccgg ggcgtgtcgg tgctgcccgg ttcggccgtg gccgccggcg
     1681 tattggacgc ggccggcgat gggcttacgg gaggctctgg cgtgtgggtg tcggtagcgg
     1741 caattgtggg gctggtcgtc cctgtgggaa agggcgtcac ctgggccacc gccgggccgc
     1801 cacaggcaac cagcgtcagg ccggtaaaca gcgccgccag aagtgacgcc agacgcaaaa
     1861 gtcccttacg agtcatagtt tggtacgatg cgcgccgccg ggtctacaac ttctcgccaa
     1921 aatatggatt cagccgcaca aagaccccat agccgatcat caggacgatg agcgatgtca
     1981 ccaacgtgcg caccagatag gcggcgttca tgcccaccgg gccgttgctg ccgtaggtgc
     2041 cccacaggac ggtgcgatag ccgtcgatga tcgaagccat cgggttgacc cagcgcatga
     2101 cctgggccgg gttgaaggtg acgcccagca cggtggccgt agggccgaac atctccagcg
     2161 agtagaacac cggcgtcatg aagaaccagg ccaggatgac gacctccagg atcatcagca
     2221 cgtcgcggta gaagacagtc aggctgccca gcagcaggca cagccccagg gtgaagatga
     2281 gctgcgtcag cagcagcacc ggcacccaca gggcgtggat cgtcaggcca atgccgaaca
     2341 cgtagaggaa gaccaccagc acggcaaagg cgaacaggaa attgaccaga ttcgacaaca
     2401 gcgccgaggt cggcaggagt tcgcgcggga agtagacttt tttcaccagg gccgagttac
     2461 cggtcaccga agtcgtccca ctggtcagcg cgccgctgaa gaagttccac ggaattagcc
     2521 cgacgaggac gaagacgggg tattgacgca acgcgctgtt ggccatcacc gtgaacagca
     2581 tggtgaagac gagcatcagg aacagcgggt tgaggatgct ccagaggatg cccaggatcg
     2641 agtttttgta gcgcgccttc aggtcgcgga cgaccagatt gcgcagcaga tagtgatagt
     2701 ggcgcaggtc ccgcagctta tcaccgacga tctcgcgcca gttagcccag cggcggtcgg
     2761 cgttgtagat gtagactggt ttgtcgccgc tttcgggccg gctgttgacg ctcatccgct
     2821 gtattgtctc ccgttgccat cgccgggcaa cgacgctgga ttatagcgcg tttggccgat
     2881 ttgccacgtc ccgcctagca ggggcatggc caggtcaata ggagctcacg caaagccgca
     2941 aagggagcca aggcgcaaag gaagaaagct tcttctttgc gccttggccc ccttggcgtc
     3001 cttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaggcg caaaggaaga
     3121 aagcttcttc tttgcgcctt ggcccccttg gcgtccttgg cgtgagcgct ttgcgtgagc
     3181 gcatcttccg acctggcact gcccctgccc ggcctaagac aggctctcgt agaggcccaa
     3241 caggcggcgg ctatggtcgg ccagggtagg cacagggcgg atgccggcgc gcagagcggc
     3301 cagccgctcc ggctgctcgg ccagcgccag cagaatatcg gccagcgccg cttcgtcagc
     3361 gggcgggaag agcagcccat cgacaccatc gcgcacgacc tcggccatga cgccaatgcg
     3421 cgaggccacc accggcacgc ccgccgcaaa ggcctcgtgg gcgatgagcg agtaggtctc
     3481 gtaccacagc gtcggcacga cgagcacatc cagcccgccc agcgccggcc agagcgccgc
     3541 ccgcgacaac cggccgggga agcggatgcc gggatgagtc gccgcggccc gcagcccggc
     3601 ggcgtagtcg ggaaaggcgc tggcgtcgcc gtagatggat agggccgcgg cctcggccgg
     3661 caaccgctgg acggcggcga cgaggtggtg gacacccttc tgcgggctga ggccgccgat
     3721 gtagccggcc cgcagcggct cgccggcggg ccgcgccgtt tgggccgcgc gcgccgcggc
     3781 caactcgtcc gcgccggcgt cgatgcccaa tggccagatg tggacgcgct ccgtcgggaa
     3841 gcccatgccg gcataggcgc ggcggacgaa cccggtcgag gccagcacgg ccgccgcccc
     3901 ggcaaacacc tgccgcagcg cccgcccgcg ccgggccatc agcggagcca ccgccggggc
     3961 cagcgctgtc agcccgccca acccggcccg cgccagggca cagcggccgc agttgacgtg
     4021 gccggcgtcc ggcccgcggc agaggcggcc gtcgtcgttg gtcaacagtt gcgcgttggc
     4081 gcagccgtac cagtagtcgt gcaggctgat gacgtagggc aggccggcgg ccttgatttg
     4141 ctccacgagg gccaggggca ggcccatcag atgttgcagg tggacgacgt cgggctgcgt
     4201 ctcggccagc gccgccgcga aactgcccgc cagccggccg ccgccgaagg tgctcagaaa
     4261 gacggccgtc cgcccacgcg ctccatcgcc agcgcggtgg acgaccagcc ggcccgtctc
     4321 gtcgtccggc gtgggtcgga cgtcgcccgg ctccgggcaa aagacagcca cggcgtggcc
     4381 ggcggcggcc tggtagccgg ccacggcccg ggtatagacc tcagtgccgc cgacgtgttg
     4441 cggagggtac tggtgaacga cgtggaggat gcgcatgggg agggaggagg gatgagggat
     4501 gagggatggg taggcttgtg gggatgcgat tcgggagaat cgcaccacat gtcagcgttg
     4561 gtggtgggcg gcgatgacct gctcgtagtg gctggccacg cgcttgacga cggtggccca
     4621 attgtagccg ctacgcagcc gttcgtagcc ctgccagccg aggcgctggg cgaacggctc
     4681 gtcgctgatg agtcgctcgg cagccgcgac cagcccctcg acgtcggcga aaggcaccag
     4741 cagcccggtc gcgccgtcat cgacgacgcc ggggatgccg cccgcccgcg cagcgatgac
     4801 cggcacgctg tggctccacg cctccaggat gacgatgccg aacgaatcgc tgcgcgaggg
     4861 cagcatcagg aacttggccc ggctgaggac ggcatgcttg tccctgtcag acaggacgcc
     4921 cagggtgcgg atggcggcgc ggtcgtgggg cgacaggccg cgccggtagc gctcgaattc
     4981 gggcgtgctg gaaccaatga gcagcagggc cacgtcgcgg ccgcgttgcc gcagggtgcg
     5041 gatggcatcg gcggcgtgca gcgcgccctt gtcgtaggac aggcggccga tgtagacgcc
     5101 gaacgttccc cgcgcctcgc agtggtcggt ggcgtagtaa ggcgaggcgc ggaagtcggc
     5161 cggcggcggg tcggcggcgc tgccgatgac gcctagccgc tccgccggca cgtggtagcg
     5221 agccaggccg tcggcttcca ccgtcgtcag cgtcagtagg cgatcggctg agcgcataat
     5281 gcgcagttgg tggctcatgg tcgagttgcg agccacgcgg tcgttgccgt gctcgcccag
     5341 gtgggcaaag ggggtgacaa cgaggggcgt gggcgacgca gccgtcgcgg tgggcgacgc
     5401 agccgtcgcg gtgggcgacg cagccgtcgc ggtaggcgac gcggccgtcg cggtgggcga
     5461 cgcggccgtc gcggtgggcg acgcggtcgt cgcggtggcc agggcgcgag cgtaggcagc
     5521 caccagccca cactcccacg aaccgttgaa ggcgtggagc acgtcggcgt ccagcccggc
     5581 cagagtctca tccaggccgt caatggccgg cacgcgccgg gccatgcgca tcagcgcgcc
     5641 ggcggggtcg gtcggcaggc tggacagcag caccatcccc ttgcgccaca acaacagacc
     5701 cgtccgcccg cctggggtgg gccgcactgg ccggcggatg acgcgcacgc catcctcgtc
     5761 cttgtccgac gtcgccggcg cgccctgcca gaagtccgcc tcggtggccg ctgagctggt
     5821 cagcacggtg acgcgccaac cagcggcaac gagaccgcga gcgaccgcgc caacgtagcg
     5881 ctcgccgccg ccgggaaaag gcgggtaagc cggggtaacg aggaccacgt ggccgttgac
     5941 gatgatgtga ctcctatggc cgcggcgcaa agatgacagg cactttggcc cgcgtgctgc
     6001 cgcgcacgat ttggaccgac acgtcaaccg aatcttcgtt atcgcggatg gtcgtgccat
     6061 ggccggtcac tgcgaccgta atcgtgccca gcgcgtgttg gcccaatccc agattatcgg
     6121 gcacgcggac atcgacggca acctgcgtcc agccggtggc cgtgccgctg gccctgggga
     6181 aagtgatgtt gctgttgctg ctgctcacgc gccagtcgta gctggtggcg ggcacgtcga
     6241 gcgtcaatat cgcccggagc tggcgcgtca ggttcggcgc gtagccgatc tcaagctcct
     6301 ccggcgatag cctgagcgtc gccgttttgg ccgcagacga cagcgcgccc gtgcgggccg
     6361 ggtcgtgctt gaacatgggc cagtcggcgc gcgtcgcccc gccgggcaag tcccagacat
     6421 agagcttgct gttgtgggcg atcagttcca actggccatc gccgtccaca tcggccagga
     6481 caggattgtt catcagcaag ccctgggcgt aatagaacgg ccggccggct gcgctggtct
     6541 tggtcagcat ctggccgttg ccgtcgatga tgccaatcga ccagccgatg gtcatgaaga
     6601 tttccatctt gccgtcgccg tcgtaatcgc ccaggatgac gcccttgccg acatcctgcc
     6661 ggtcggtgtc gccgaagggg ctgcgcggcg tcatcggaaa gccgctgacg agactgccat
     6721 cggcgtgcca ggcgaagaga cggccctgta acgtggccac gacgatctcc ggcgcgctgt
     6781 cgccagcgat gtcgccaacg gcggccgacc cgggcacaag gtcgcccgtc ggctggccgc
     6841 cgccccagcc gggcagttcg cgcccctggc tgtcgaaggc gtagaagcgc tggccgtagg
     6901 tcggccggtc ggggctgttg ttgtagtaga aagtgcccat gccgacgaaa atctccggcc
     6961 gggcgtcgcc ggtcacgtcg gccagcgccg gcgtcgattg gatgatctcc agccggtatt
     7021 gcgggaagcc gggcagcagc gcgccgccgc cggtcaggcc atagagcgag ccaccgcaat
     7081 agccctccat ccagccgccc ggcagccgat aggggcatgt ccagccgccg gagtcgcctg
     7141 gctggctgct gtcgatattg ccttcgtcgc tgccgatgag gatgtcgagg cggccgtcgc
     7201 cgttcatatc ggccagcgcc ggcgaactcc agatgctgtc ggccaggcga ttgtgtaggt
     7261 tctcccagcc caagcgggcg tagagcgcgc tcggcggcgg gaagccggcc acacgctggc
     7321 cagtgtggtg gaaagcgtag atgcgtttgt cgatgccgcc gatgacgatc tccaggtcgc
     7381 cgtcgccgtc caggtcgccc agcgccgggg tgctgatgaa gggggcaggg cagtg
//
LOCUS       DAOSMQ010000100         7371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10105_length_7371_cov_4.126435, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000100 DAOSMQ010000000
VERSION     DAOSMQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10105_length_7371_cov_4.126435"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PK829_04820"
     CDS             complement(<1..127)
                     /locus_tag="PK829_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOU40569.1"
                     /translation="MALITMRQAITDALREEMHRDERVFIMGEEVGVWGGTYAVTR"
     gene            complement(175..1131)
                     /gene="pdhA"
                     /locus_tag="PK829_04825"
     CDS             complement(175..1131)
                     /gene="pdhA"
                     /locus_tag="PK829_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOU40570.1"
                     /translation="MEKETLLDWYRQMVLGRRFEQKCAELYQLGKIGGFLHLYIGQEA
                     TAVGSINARRPQDHVITAYRDHVHALVSGAAPKRLMAELLGKDTGVSRGKGGSMHLAS
                     VEHNYWGGYGIVGGHIPLGTGLALAEQYKGTDAVVLCYMGDGSTNIGYFHESLNMAGV
                     WDLPVVYIVENNKYGMGTSEARASAGTMLAKAAAYGMTGRQVNGMNVMEVYEATREAI
                     DAIHAGHAPQFLEVITYRYEGHSMGDPLRYRTKEEVEKWRAEDPIGILERYLYSEKMA
                     TEADLDKIEDEVEKVIAEAVEFAEQSPFPAPEALFEGIYVDN"
     gene            complement(1263..2237)
                     /gene="thrB"
                     /locus_tag="PK829_04830"
     CDS             complement(1263..2237)
                     /gene="thrB"
                     /locus_tag="PK829_04830"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOU40571.1"
                     /translation="MPVDSRSVTVSVPATSANLGPGFDCLGLALELRNEVTFTAGGPL
                     LPHNSPQPTAYHFTVSGIDANKVPSDGRNLLIQAADTLFQRAGRRPADVSVSVLNRIP
                     VGSGLGSSSAAIVAGLLAANAVSEAGWTQDRLLRLAVEMEGHPDNVAPALLGGLVLGV
                     LPDATYGPAEIILRRIPAPRPAVVVVLPDFPFLTADARAALPPTVPRADAIFNASRVA
                     LLLHALTSDDAECLRVAMGDRLHQPYRLPLIPGALAAYEAAYAAGALGVALSGAGPSL
                     LAFVAGNSADNGAAVGRAMTDAFAAAGLSSRTWLLRASAAGARLSVEN"
     gene            2303..3352
                     /locus_tag="PK829_04835"
     CDS             2303..3352
                     /locus_tag="PK829_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU40572.1"
                     /translation="MKRPWVNLLLLLLLTAQGITGYFGFTNGREADAWILQLHGIVAY
                     GLVLLFFAKATIVLDAWRRKRRWTGRRIGFAVALALFVLTLLSGLLWTFHGPLYWGGF
                     SLVSLHIYIAVPLMGLMLWHAWHMRTVRRVPGAAGRRLFLGGLATLSGGLLFWAVVGR
                     ANAALGLSGAGRRFTGSYEIGSHTPDFPVVSWIADRPPPIDREAWRLRVEGAVARPLS
                     LTYEELAAMAQSEVAAAIDCTGGWYSVQRWRGVVVGELLARAGVLPAAASVTFEAVSG
                     YRRRFPVDEATAALLALGTLGQEDNAFHPLAHGHGAPARAVLPGRRGVEWVKWLAVIR
                     VNETGPWLQSPLPLQ"
     gene            complement(3365..4399)
                     /locus_tag="PK829_04840"
     CDS             complement(3365..4399)
                     /locus_tag="PK829_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOU40573.1"
                     /translation="MSNSQLSPNGNAPLGPARHELTPVNYGGQAVIEGVMMRGSRALS
                     VAVRNPQGDIVVHTEPLDSRIYGGRLARIPFLRGLTLLWDALGLGIKSLMFAAEVALQ
                     DESQTSTDGKPEKVFEGPVQWTMVIFSLTMSILLFFVLPTFLAHLIEGALGLSGQPVA
                     INFIEGMIRLVLLVGYIWGIGRMADIKRLFGYHGAEHKTINAYEAGAELTPVSVARFP
                     LEHPRCGTAFLLTVVVISILLYSLLPPLDLLPRILSRLVLLPVIAGIAYEFLKFTASR
                     QGNPLIRLLTKPNLAMQRLTTREPDMDMLAVAIAAFENVLAYERSQTAVASGDMVIAG
                     GAAAAAEPAA"
     gene            complement(4433..4975)
                     /locus_tag="PK829_04845"
     CDS             complement(4433..4975)
                     /locus_tag="PK829_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOU40574.1"
                     /translation="MSYATVLYFDPATEDAVYSVWDALNAVVDQPMRDLGIRPHVTLT
                     SGDQIDQGALELALATFAASIHPFRLTLSSVGLFATGEGVLFFGVTVSRALLELHAEY
                     SRVFGRYVRQANPYYRLGAWVPHCTLTMGLQPQQVGEAVAIARRAPLPLHGNVAEIAL
                     VRVPDGPVETLGLFQLGARA"
     gene            complement(4988..6061)
                     /locus_tag="PK829_04850"
     CDS             complement(4988..6061)
                     /locus_tag="PK829_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40575.1"
                     /translation="MTVTEIRAFPVSSTVVMRSTLFALANLGATLQTYNEDSGVIVAT
                     VSRWLGLQRQEVVARVRSFEQTCQLELDAPDVEKARELLQLIATYVRDGGRVQANATM
                     QWVDLQRQQANKARRQELGAKARTLLGSGSESNLPAVVEEGANLPAVANDDPAQPGPS
                     LSAPVAIPDNPGVLVKNNRQMLLELKVDPEVFTDRSGYVSMCSVCYAPALRGSAFCPN
                     CGRPLTLEAVQPELRGNAQKSARSGLTYGLVGLAFNVIPLLVLVLPALLAEPVDAGLL
                     DRIRETLSPLTIALAAIVGIAPAMVAGFLALRQGQRAAWFLNLPAVLEQNGRGQASLG
                     TALGWLSIYLGVAWILLIVIALL"
     gene            complement(6221..7342)
                     /locus_tag="PK829_04855"
     CDS             complement(6221..7342)
                     /locus_tag="PK829_04855"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOU40576.1"
                     /translation="MTTILILGGYGQAGRALAECLLAQTDCRLILAGRRPERAAPLVA
                     ALNEAAGAARVTAVRADAARPEELARALAGVDLLLVASSTAAHTETVARAALAAGCDY
                     LDIQYAAAKVACLRRLAPEIERAGRCFITDGGFHPGVPAALVRYAATCFDRLERANVA
                     SVIAVDWAALDLGEETREEFVAEFMDFDGRVFRDGCWQSSGLASMVRPLTVSFGPPFG
                     RRACVPMGLAEMDDLPEQIPSLRETGFYVGGFNWFTDWLVSPMVVLGLKVAPCRLLGS
                     MARLLFWSMCRFARPPYGMVLQLEAAGQRAGAAHTMTLRLAHPDGYAFTAIPTVACLR
                     QWLAGARPAGLWLQAQFVAPEPFLRDMAAMGVVMTQVNG"
BASE COUNT         1236 a         2386 c         2537 g         1212 t
ORIGIN      
        1 cgcgcgtgac agcgtaggtg ccgccccaca caccgacctc ctcgcccatg atgaacacgc
       61 gctcgtcgcg gtgcatctcc tcgcgcaggg cgtcggtgat cgcctgtcgc attgtaatca
      121 gtgccatgct atctcctgtc ctatctgatg ggccgcccag cgaccgcgta gtggctagtt
      181 atcgacgtag ataccctcga acagcgcctc cggggccggg aagggcgact gctcggcgaa
      241 ttcgactgcc tcagcgatga ccttctcgac ctcatcttcg atcttgtcca ggtcggcctc
      301 ggtagccatc ttctcgctgt agagatagcg ttcgagaatg ccaatggggt cttcggcgcg
      361 ccacttctcg acctcttcct tggtgcggta gcggaggggg tcgcccatgc tgtggccctc
      421 gtagcggtag gtgatcactt ccaagaactg cggcgcgtgc ccggcatgga tggcatcaat
      481 cgcttcacgc gtggcctcgt agacctccat gacgttcatg ccgtttacct gccgcccggt
      541 catgccgtag gccgcggcct tggccagcat tgtcccggcc gaggcgcggg cctccgacgt
      601 gcccatgccg tacttattgt tctcgacgat gtagacaacc ggcaagtccc agacgccggc
      661 catgttgagc gattcatgga agtagccgat gttggtcgag ccgtcgccca tgtagcacag
      721 gacaacggcg tcggtgcctt tatactgctc ggccagggcc aggcccgtgc ccaacgggat
      781 gtgcccgcca acgatgccat agccacccca gtagttatgt tccaccgagg ccagatgcat
      841 cgagccgccc ttgccccggc tgacgccggt gtccttgccc agcaactcgg ccatcagccg
      901 cttcggggcc gcgccggaca cgagggcgtg gacgtggtcg cgataggcgg tgatgacgtg
      961 gtcttgcggg cggcgggcgt tgatggagcc gacggcggtc gcctcctggc caatatagag
     1021 atgcaggaag ccaccgatct tgccgagttg gtagagttcg gcgcactttt gctcgaaacg
     1081 acggccaagt accatctgac ggtaccagtc caggagagtc tccttttcca ttcaattctc
     1141 cttcacgagg tgatgccggc cgatttgagc gctcggccgc ggtcgataga aagtgtaaca
     1201 aaaggtcaac aagctgacca aagcatttca tcgcaccgag gcagcgcgac gttcgccagg
     1261 cgctagttct ccacggacag ccgagcgccg gcggccgacg cccgcagcag ccaggtgcgg
     1321 ctggacagac cagcggcggc aaaggcgtcg gtcatggccc ggccgacggc ggcgccattg
     1381 tcggcgctgt tgccggctac aaaggccagc aggctcggcc ctgcgccgct gagagccacg
     1441 cccagcgccc cggcggcata ggcggcttcg taggccgcca gcgcgccggg aatgagcggc
     1501 agccggtagg gttggtgcag ccggtcgccc atagccacgc gcaagcattc ggcatcgtcg
     1561 ctcgtcagcg cgtgcagcag cagggccaca cggctggcgt tgaaaatggc gtcggctcgc
     1621 ggcacggtcg gtggcagggc ggcgcgcgcg tcggcggtca ggaaggggaa gtcgggcagg
     1681 actactacga ccgccggccg cggcgcaggg atgcgccgca ggatgatctc ggccgggccg
     1741 taggtcgcgt cgggcaagac gcccagcacc agcccgccca acagcgccgg ggcgacatta
     1801 tcagggtgac cctccatctc tacggccagt cgcagcagcc gatcctgcgt ccaacctgcc
     1861 tcgctgacgg cgttggcggc cagcagcccg gcgacgatgg ccgcggaact actgcccagc
     1921 ccactgccca cgggaatgcg gttgagcacg ctcacggaca cgtctgctgg ccgccggccg
     1981 gcgcgctgga agagcgtgtc ggcagcctgg atgagcaggt tgcggccgtc ggacggaacc
     2041 ttgttggcgt cgataccgct gactgtgaag tgataggcgg tcggctgggg gctgttatgg
     2101 ggcagcagcg gcccaccggc agtaaaagtg acctcattcc gcagttccag cgccagcccc
     2161 aagcagtcga aacccggccc caggttggcg ctggtggccg gcacggaaac cgtaacgctg
     2221 cgtgagtcga cgggcatcgt tgtagacatg cgcccgtagt ttacactaga atggcggcta
     2281 ataccctttt gggacagaga caatgaagcg cccgtgggtc aacttgcttc tgctactcct
     2341 gctgacggcg cagggcatca ccggctattt cggctttacc aacggccggg aggcggacgc
     2401 ctggattttg cagctgcacg gcatcgtcgc ctatggcctg gttctgcttt tcttcgccaa
     2461 ggccaccatt gtcctcgacg cctggcggcg caagcggcgc tggacaggcc ggcggatcgg
     2521 ctttgccgtg gcgctggcgc tgtttgtgct gacgctacta tcggggctgt tgtggacgtt
     2581 tcacggcccg ctctattggg gcggtttcag cttggtcagc ctgcacatct acatcgctgt
     2641 gcccctgatg ggcctaatgc tctggcatgc ctggcacatg cgcactgtgc gacgcgtgcc
     2701 aggggccgcc gggcggcggc tgtttctggg cgggttggcg acgttgtccg gcgggctgct
     2761 gttctgggct gtggttggcc gggccaacgc cgctcttggt ctatccgggg cgggccgccg
     2821 cttcactggc tcctatgaga tcggcagcca cacgcccgat ttccccgtgg tcagttggat
     2881 cgccgaccgg ccgccgccca tcgaccggga agcctggcgc ttgcgggtcg agggggccgt
     2941 ggcccggccg ctgtcgctga cctacgagga gctggccgcg atggcgcaat cggaagtggc
     3001 cgcggcgatc gattgcaccg gcggctggta ctctgtccag cgctggcgcg gcgtggtcgt
     3061 gggcgagttg ctggcgcgag cgggcgtgct gccagcggcg gccagcgtca ctttcgaggc
     3121 cgtttctggc taccggcggc gtttccccgt ggatgaggcc acggcggcgc tattggcgtt
     3181 gggcacactg gggcaagagg acaatgcgtt tcacccgctg gcccacggcc acggcgcgcc
     3241 ggcgcgcgcg gtgctgcccg gccgccgggg cgtggagtgg gttaagtggc tggccgtcat
     3301 ccgcgtcaac gaaacggggc cgtggttgca gtcaccgctg ccgttgcagt gaggccgggc
     3361 cgcgctaggc cgccggttct gccgccgcgg ccgctccgcc agcgatgacc atatcaccac
     3421 tggccacggc cgtctggctg cgctcatagg ccagcacgtt ctcgaacgcg gcgatggcca
     3481 cagccaacat gtccatgtcc ggctcgcgcg tcgtcagacg ctgcatggcc agattgggct
     3541 tggtcagcag ccggatgagt gggttgccct gccgagaggc ggtgaacttg aggaactcat
     3601 aggcgatacc ggcgatgacc ggcagcagca ccagccgcga taagatgcgc ggcaagaggt
     3661 ctagcggcgg cagcaggcta tagagcagga tggagatgac gacgacggtc agcaggaagg
     3721 ctgtgccgca gcgcgggtgc tccagcggga agcgggccac ggagacgggc gtcagctccg
     3781 ccccggcttc gtaggcgttg atggtcttgt gctccgcgcc atgatagccg aacagtcgct
     3841 tgatgtcggc catgcggccg atgccccaga tgtagccaac caacagcacc agccggatca
     3901 tgccctcgat gaagttaatg gccaccggct ggccgctcag ccccagcgcg ccctcgatca
     3961 gatgagcgag aaaggtgggc agaacgaaga agagcaggat cgacatcgtc aacgagaaga
     4021 tgaccatcgt ccactgcacc ggcccctcaa agactttctc tggcttccca tcggtgctgg
     4081 tctggctctc gtcttgcagc gccacctcgg cggcaaacat caggctctta atgcccaacc
     4141 ctagcgcgtc ccacagcagc gtcaggccgc gcaggaaggg gatgcgcgcc agccggccgc
     4201 cgtagatacg gctgtctagc ggctcggtgt ggacgacgat gtcgccctgt gggttgcgca
     4261 cggccacgga tagcgcccgc gagccgcgca tcatgacgcc ttcgatgacc gcctggccgc
     4321 cgtagttcac cggcgtcagt tcgtggcggg ccggccccag cggggcgttg ccattgggag
     4381 aaagctgact gttcgacatt gggccgattg tagcaggcgg cgggctggtt gcctatgccc
     4441 gcgcgccaag ctgaaaaagg cctaatgtct ctactggccc atcgggcacg cgcaccaggg
     4501 ctatttcggc cacgttgccg tggagcggca gcggcgcgcg ccgggcgatg gccacggcct
     4561 cgcccacctg ttggggctgt aggcccatcg tcagcgtgca gtggggcacc cacgcgccca
     4621 accgatagta gggattggcc tggcgcacgt agcggccgaa aacgcgactg tactcggcgt
     4681 gcagctccag cagggcgcgg ctgaccgtca cgccgaagaa gagcacgcct tcgcccgtgg
     4741 cgaacaggcc gacgctcgac agagtcaggc ggaacgggtg tatggaagcg gcgaaggtgg
     4801 ccagcgccag ctctaacgca ccctggtcga tctgatcgcc gctggtcaga gtaacgtgcg
     4861 gccggatgcc gaggtcgcgc atgggctggt cgaccacggc gttcagggcg tcccagacgc
     4921 tatagacggc atcttcggtc gccgggtcga agtacaggac agtcgcgtag gacatgggga
     4981 acctccgcta gaggagcgcg atgacgatca gcagaatcca ggccacgccc aggtagatgc
     5041 tcagccagcc gagcgccgtg cccagactgg cctggccgcg gccattctgt tccaacacgg
     5101 ccggcagatt gagaaaccag gcggcgcgct gtccctgtcg cagcgccagg aagccggcga
     5161 ccatggccgg agcgatgcca acgatggccg ccagggcgat agtcagcggg ctgagggtct
     5221 cgcggatgcg gtcgagcaag ccggcgtcga ccggttcggc cagcagggcg ggcaggacga
     5281 ggacgagcag cgggatcacg ttgaaggcca ggccgactag cccgtaggtc aggccagagc
     5341 gggctgactt ctgggcgttg ccgcgcaatt ccggctgcac cgcctccagc gtcagcggcc
     5401 ggccgcagtt ggggcagaag gcgctgccgc gcagggcggg agcgtagcag acgctgcaca
     5461 tcgacacgta gcccgaacgg tcggtgaaga cttccggatc gaccttcagt tccaacaaca
     5521 tctggcggtt gttcttgacg agaacgccgg ggttgtcggg gatggccacc ggcgcggaca
     5581 gggatgggcc gggctgcgcc ggatcatcat tggccacggc gggcagattg gcgccctcct
     5641 cgacaacggc cggcaggttc gactcgctgc cgctgccgag cagggtgcgc gccttcgcgc
     5701 ctagctcttg ccggcgggcc ttgttggcct gttggcgctg caagtccacc cactgcatgg
     5761 tggcgttggc ctgcacccgg ccgccgtcac gcacgtaggt ggcgatgagt tgcagcaact
     5821 cgcgcgcctt ctccacgtcg ggcgcgtcta gttccagctg gcacgtctgc tcgaaggagc
     5881 gcacgcgggc gacgacctcc tggcgctgta aacccaacca gcgcgagacg gtggcgacga
     5941 tgacgccact gtcctcgttg taggtctgca aggtggcccc caggttggcc agggcaaaga
     6001 gcgtgctgcg catgacgacg gtggaactga ccggaaaggc gcgaatctcg gtaacggtca
     6061 tagtggggct gattatagcc gatgtattgc cagtgaacag tgaacagtgg acagtgaaca
     6121 gtgaacagta ggggaccact gttcactgtt cactgcttac tgtccactgc ttactgtcca
     6181 ctgcttactg tccactgctt actgtccact cctccgcaag ctatccgttc acttgagtca
     6241 tcaccacgcc catcgcggcc atgtcgcgca ggaacggctc cggggcgacg aattgggcct
     6301 ggagccacag gcccgccggc cgcgccccgg ccagccactg acgcaggcag gcgaccgtgg
     6361 ggatggcggt gaaagcgtag ccgtcggggt gagccaggcg cagggtcatg gtgtgggccg
     6421 cgccggcgcg ctgcccggcg gcctccagtt gcagcaccat gccatacggc gggcgggcga
     6481 agcgacacat actccagaac agcagtcggg ccatcgaccc cagcaggcgg catggcgcga
     6541 ccttcaggcc gaggacgacc ataggcgaga cgagccaatc ggtgaaccag ttgaagccgc
     6601 cgacgtagaa gcccgtttcg cgtagcgacg gaatttgttc gggcaggtcg tccatctcgg
     6661 ccaggcccat cgggacgcaa gcgcgccggc cgaagggtgg cccgaacgac actgtcagcg
     6721 gtctgaccat cgacgccagc cctgacgact gccagcagcc gtcgcggaag acgcggccgt
     6781 cgaagtccat gaactcggct acgaactcct cgcgcgtttc ctcccccagg tcgagcgccg
     6841 cccaatcgac ggcgatgacg ctggccacat tggcgcgctc cagccggtcg aaacaggtgg
     6901 ccgcgtagcg caccagcgcg gcgggcacgc caggatggaa gccgccatcg gtgatgaagc
     6961 agcggccggc gcgctcgatc tctggggcca gccgccgcag gcaggccacc ttggctgcgg
     7021 cgtactggat gtcgagatag tcgcagccgg cggccagcgc cgcgcgggcg accgtttcgg
     7081 tgtgggcggc cgtgctggag gcgaccagta gcagatcgac gccggcgagg gcgcgggcca
     7141 gttcctccgg ccgggcggcg tcggcacgca cggccgtcac ccgcgccgcg ccggcggcct
     7201 cgttgagcgc ggcaaccaac ggcgcggccc gctccggccg ccgcccggcc aggatgagac
     7261 gacaatcggt ctgtgccagc aagcactcgg ccagcgcgcg accggcctgc ccatagccgc
     7321 cgaggatgag aatcgttgtc atcgttgcgc ctccacccac ctggattgcc g
//
LOCUS       DAOSMQ010000101         7365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10114_length_7365_cov_4.427223, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000101 DAOSMQ010000000
VERSION     DAOSMQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10114_length_7365_cov_4.427223"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PK829_04860"
     CDS             complement(<1..1048)
                     /locus_tag="PK829_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU40577.1"
                     /translation="MSMAQQDTLAARPPYGRVAFDAMAAPAAVVVVGQARFTVLTPRA
                     IRMEYSPGGAFEDRPSQVFWHRRQPVPPFEARRDGDGLTLTTDALRLRYSGGAFAPAT
                     LSVTLLPDGPTWRYGDADTGNLRGTTRTLDMVDGPTPLAPGLLSRDGWAVIDDSHTLV
                     FDDAGWLCARSEPGARDLTFLGYGRDYRAALRDYTALSGRAPLLPRWALGNWWSRYWA
                     YSDADLRALMDDFRAHDVPLSVCIIDMDWHLVDVGEGISGWTGYTWNHALFPDPPGFI
                     AWLHAQGLRTALNLHPALGIRPHEAAYGEMCRRLEIDLSAGHSIPFDIANPRFADAYF
                     EVLHHPLEDIGVDFW"
     gene            1166..2203
                     /locus_tag="PK829_04865"
     CDS             1166..2203
                     /locus_tag="PK829_04865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HOU40578.1"
                     /translation="MTTTALLLSDAQAYKFAPEPVYHGPELLACLQDLATRARAAGAP
                     VIFVRHNGRPGDPDAPNTRGWEIHPALAVAANESIIDKHTPDAFYQTRLAGVLAEQGI
                     GRLVIAGMQTEYGIDTTVRRARSLDYEVVLAADGHSTYPGALTAGQIIAHHNNVLAAF
                     ADVRPAAEIDFAAPPAPDVRAEGLTAADLSAIGTGLDEWRVYEQWLATGEGHPYWPHT
                     HPARVSDMLKSLWDAGFKPRARYTDPPRWEMGVARVFMQPLENIPLVFRRASLQAVIG
                     AMDHLLQNPRNPLSPHIRQIGEQVWLYDARDLRLVYVPSVVNDKEGRERRHIFLLWLA
                     PGIQVRNPFLP"
     gene            2200..3036
                     /locus_tag="PK829_04870"
     CDS             2200..3036
                     /locus_tag="PK829_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HOU40579.1"
                     /translation="MTAVPLLMAFVSDLMFTTRIARAAEHLGYRVEWIETADAFGSDA
                     DAREETPGELLHGRTGRLFEQVTRAQPALLLFDLNNAAIPWREWIAALKSSAATRRIP
                     VLCFGPHEDVATMTAARAAGADAVLARSRFTADMPGLIAQYARVPDTVALGEACAEPL
                     SDLARTGIERFNAGDYYHAHDALEEAWMLDRGAGRELYRGLLQVGIAYYQIERGNYRG
                     AVKMLLRARQWLEPLPAVCRGVDVATLRADAERVYAALAAAGPEGVGAIDRALLCPIL
                     LF"
     gene            3044..3784
                     /locus_tag="PK829_04875"
     CDS             3044..3784
                     /locus_tag="PK829_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOU40580.1"
                     /translation="MSQTYVPKTAMFIFAHPDDIEFGVAGTAALWARGGCRVIYVVIT
                     DGNVGSHEDGMTPERLAEIRRAEQTAAGDVAGAECVYLGYHDGFLQPTLELRKDLVRL
                     IRQYRPEAVVCLDPTNFFPAEDYINHPDHRAAGQAALDAVFPAAEMTLLYPDLAAEGL
                     TGHKVDYVFITFSERANYYVDITTTLDLKIEALRKHASQLGKWDPSPMIRQWAAESGK
                     KVGFAYAEAFYRITLRQVETPAEPQPEV"
     gene            3788..5008
                     /locus_tag="PK829_04880"
     CDS             3788..5008
                     /locus_tag="PK829_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU40581.1"
                     /translation="MDSEPFVLGVNYWPRRKAMYWWSDFDPGEVRAEFALIRELGLRL
                     VRIFLLWDDWQPAPDHVSPAALVHLAQVMTIAADLGLKLDVTFFTGHMSGPNWSPGWL
                     LELDKPLPARVRQLVSGGRVVDCGYRNVYTDPIALEASELLLRTVVGRFHEHPAVGLW
                     NLGNEPDLFAWPPNAAAGREWVRRMTGLIKELDPTRPVTCGLHVDSVFRDNGLRIHDV
                     FAEVDIPVMHGYPMYIDWLDDPLDVDFVPYLCALTTALCGKPTLMEEFGGCTNSPGEP
                     SAYWEWSAYGEPRRQFMASEEALADYITAVLPRLVEVGATGALLWCFADYVPELWDRP
                     PCEQSIHERFFGLVRPDGTVKPHALALQAFANTRPTVQPATRPLTLDISPEEYYRDPA
                     GHAARLYRVFRGQS"
     gene            5112..6116
                     /locus_tag="PK829_04885"
     CDS             5112..6116
                     /locus_tag="PK829_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40582.1"
                     /translation="MPRKKILFIGGSLNMTRMTHAVARHLADEFECWFSPFYSDGLYD
                     ILASHSHLLDFTVLGGNFRRQTDAYLRQHNLPIDYRGAAREYDLYVATTDLYVPKNLR
                     RRPFILIQEGMTDPEGFMYHMVKWFRLPRYLASTSTNGLSHQYQYFCVASEGYRDFFI
                     RKGVRPEKLIVTGIPNFDNIATNLDNDFPQRGYVLVATSDARETYKFDNRRKFLRQAV
                     DIAAGRPMIFKLHPNENFKRATREIREVVGDVPIYTDGNIDHMIANCDTLICQYSTVV
                     YVGIALGKEVYSYFDVDTLRRLTPLQNGGQSGRNIAQVCRHILLGEPVNWEEFLGQAV
                     "
     gene            6100..6915
                     /locus_tag="PK829_04890"
     CDS             6100..6915
                     /locus_tag="PK829_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOU40583.1"
                     /translation="MARLSSPGGAPPHRVVTVVQARTGSTRLPNKVLRPLAGSPLLVR
                     MVERVRAARLAGTVVVATTTAPDDDPIADLCRAEGWPCFRGDPLDLLDRHYQAGLAYG
                     ATAVAKIPSDCPLIDPAIIDRVLGHFLDHPGAFDFVSNLHPASYPDGNDVEIMTLDAL
                     ATAWREATRPLEREHTTPYLWENPDRFRLGNVLWETGLDYSKSHRWTIDYEEDYQFIR
                     AVYEALYPANPRFGLGDILAYGESHPHVAALNSAYAGEYWYRHHPGELKTVSG"
     gene            6964..>7365
                     /locus_tag="PK829_04895"
     CDS             6964..>7365
                     /locus_tag="PK829_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU40584.1"
                     /translation="MTQEEIEQLQEMALRVREHIIRLSTDGGCFTGASLSCADLIVYL
                     YNRFLNVGPDNFDAPDRDYLFLSKGHDVPALYGTFVELGWLAAERLGNHLKTNDFIYW
                     HPNRAIPGIEFHSGSLGHLPSVAVGVALDCKL"
BASE COUNT         1212 a         2645 c         2289 g         1219 t
ORIGIN      
        1 accagaaatc gacgccgatg tcctccagcg ggtgatggag tacctcgaag taggcatcgg
       61 cgaagcgcgg gttggcgatg tcgaaaggga tggagtggcc ggcgctcagg tcgatctcca
      121 gccggcggca catttcgccg taggcggcct cgtgcgggcg gatgcccagg gccgggtgca
      181 ggttcagcgc cgtccgcagc ccctgcgcgt gcagccaggc gatgaagccg ggtgggtcgg
      241 ggaagagggc gtggttccag gtgtagcccg tccaaccact gatgccctcg cccacatcga
      301 cgagatgcca atccatgtca atgatgcaga ccgacagggg cacgtcgtgg gcgcgaaagt
      361 cgtccatcag cgctcgcagg tcggcgtcgc tgtaggccca gtagcggctc caccagttgc
      421 ccagcgccca gcgcggcagc agcggcgcgc gcccggacag agcggtatag tcgcgcagag
      481 ctgcccggta gtcgcggccg tagcccagga aagtgaggtc gcgcgcccct ggctccgagc
      541 gcgcgcacag ccagccggcg tcgtcgaaca ccagcgtatg gctgtcgtcg ataaccgccc
      601 agccgtcgcg cgacaacagg cccggggcga gtggcgtagg gccatcgacc atgtccagcg
      661 tgcgcgtcgt gccgcgcagg ttgcccgtgt cggcgtcgcc gtagcgccac gtcggcccgt
      721 cgggcagcag cgtcacgctc agcgtcgccg gggcgaacgc gccgccgctg tagcgcagcc
      781 gcagcgcgtc ggtggtcagc gtcaggccgt cgccatcgcg ccgcgcctcg aagggcggca
      841 caggctggcg gcgatgccag aagacctggc tggggcgatc ctcgaacgcg ccgccggggc
      901 tgtactccat gcggatcgcc cgcggcgtca gcaccgtaaa acgcgcctgc ccgacaacga
      961 cgacggcggc cggggcggcc atcgcatcaa aagccacacg gccatagggc ggtcgcgcgg
     1021 cgagagtgtc ctgttgcgcc atactcatac gtcccattat agccagtcgc cagatggtgt
     1081 ggaataggcc atcggcactc tccgccggcg cgggccgcgg cgcgttagcc tttgttctgt
     1141 ttctgaccta cactataatg gctccatgac cacgacggcc ctcctcctca gcgatgccca
     1201 ggcctacaaa ttcgcgccgg agccggtcta tcacgggccg gaactcttgg cctgtctgca
     1261 agacctggct acgcgggcgc gcgctgccgg cgcgccggtc atctttgtgc gccacaacgg
     1321 ccggcccggc gaccccgacg cgcccaacac ccggggctgg gaaatccatc cggcgttggc
     1381 tgtcgcagcc aacgagtcga tcatcgacaa acacacgccc gacgccttct atcagacgcg
     1441 gttggccggc gtgctggctg agcagggcat cggccggctg gtcatcgccg gaatgcagac
     1501 ggagtacggc attgacacca ctgtccgccg cgctcgtagc ctcgactacg aggttgtcct
     1561 ggctgccgac ggccacagca cctaccccgg cgcgttgaca gccgggcaga tcattgccca
     1621 ccacaacaac gttctggccg cgtttgccga cgtgcgcccg gcggcggaga tcgacttcgc
     1681 cgccccgccc gcgccggacg ttcgcgccga gggcctgacg gcggccgacc tgtccgccat
     1741 tggcacgggg ctggacgagt ggcgcgtcta tgagcaatgg ctggccaccg gggagggcca
     1801 cccctattgg ccgcacaccc atcctgcccg cgtcagtgac atgctcaaga gcctgtggga
     1861 cgcaggcttc aagcctcgcg cccgctacac cgacccaccg cgctgggaaa tgggcgtcgc
     1921 ccgcgtcttc atgcagccgc tggaaaacat tcccctggtc ttccgccgcg cctcgctcca
     1981 ggcggtcatt ggcgcgatgg atcatctctt acagaatccg cgcaacccgc tctcgcccca
     2041 tatccgccag atcggcgagc aggtttggct ctacgacgcc cgcgacctgc ggctggtcta
     2101 cgtgccctcg gtcgtcaacg acaaagaggg ccgcgagcgc cgccacatct tcctgctgtg
     2161 gctggctccc ggcattcagg tacgcaaccc cttcttgcca tgaccgctgt gcccctgttg
     2221 atggccttcg tcagtgatct gatgttcacg acgcgcatcg cccgcgccgc cgagcacctg
     2281 ggctaccgcg tcgagtggat cgagacggcc gacgcgtttg gctccgacgc cgacgcccgc
     2341 gaggagacgc ccggcgagtt gctccacggc cggacggggc ggctcttcga gcaagtgacg
     2401 cgcgcccagc cggcgctgct gctgttcgac ctgaacaacg ccgccattcc ctggcgggag
     2461 tggattgccg ccctcaagtc gtcggcggcc acgcggcgca tccccgtcct ctgctttggc
     2521 ccgcacgagg acgtggccac catgaccgcc gcccgcgccg ccggcgccga tgccgtcctg
     2581 gcccggtcgc gcttcaccgc cgacatgccc ggcctcatcg cccaatacgc ccgcgtaccc
     2641 gacacggtcg cgttgggtga agcttgcgcc gaaccactgt ccgacctggc ccgcactggc
     2701 atcgagcgct tcaatgccgg cgactactac cacgcccacg acgcgctgga agaggcctgg
     2761 atgttggacc gcggggccgg gcgagagctt taccgcggcc ttctccaggt cggcatcgcc
     2821 tactaccaga tcgagcgcgg caactaccgc ggcgcggtca agatgctact gcgggcgcgg
     2881 cagtggctgg agccgctgcc ggccgtctgt cgcggcgtcg acgtggccac gctgcgggcc
     2941 gacgctgagc gggtctacgc ggccctggcc gccgctggcc cggagggcgt cggcgcgatc
     3001 gatcgcgccc tgttgtgtcc tatcctgttg ttctagaggt ctcatgagtc aaacatacgt
     3061 tcccaagacg gcgatgttca tcttcgccca tcccgacgac atcgagtttg gcgtggccgg
     3121 cacggcggcg ctgtgggcgc ggggtggctg ccgcgtcatc tacgtggtca tcaccgacgg
     3181 caacgtcggc agccacgagg acggcatgac cccggagcgg ctggccgaga ttcggcgcgc
     3241 cgagcagacc gccgcgggcg atgtcgccgg ggcagagtgc gtctacctcg gctaccacga
     3301 cggctttctg caaccgacgt tggaactgcg caaggacctc gtgcggctca ttcgccagta
     3361 ccggccagag gccgtcgtct gcctcgaccc gaccaacttc ttcccggccg aggactacat
     3421 caaccatccc gaccaccgcg ccgccggcca ggccgccctc gacgccgtgt ttccggcggc
     3481 ggagatgacg ctgctctacc ccgacctggc cgccgagggc ctgaccggcc ataaggtcga
     3541 ttacgttttc atcaccttta gcgagcgggc caactactac gtcgatatca ccacgacgct
     3601 tgacctcaag atcgaggccc tgcgcaaaca tgccagccag ttgggtaagt gggacccgtc
     3661 accgatgatt cgccagtggg cggccgagtc gggcaagaag gttggctttg cctatgccga
     3721 ggccttttac cgcattacct tgcggcaggt tgagaccccg gccgagccgc aaccggaggt
     3781 ctagcccgtg gacagcgagc cgtttgttct cggcgtcaac tattggccgc gccgcaaggc
     3841 gatgtactgg tggtcggact tcgaccccgg cgaggtgcgc gcggagttcg ctctcatccg
     3901 tgaactgggg ctgcgcctgg tgcgcatctt cctgctgtgg gacgactggc agcccgcgcc
     3961 cgaccacgtt agcccggccg ctcttgtcca cctggcccag gtgatgacca ttgccgccga
     4021 cctgggcctg aaactggacg tgaccttctt cactggtcac atgagcggcc ccaactggtc
     4081 gcccggctgg ctgctggaac tggacaagcc actgccggcg cgcgtgcgcc agctggtcag
     4141 cggcggacgg gtcgtcgatt gcggctatcg caacgtctac accgacccga tcgctctgga
     4201 ggcatcggag ctgctgctgc ggacagtcgt cggtcgcttc cacgagcacc ccgctgtcgg
     4261 cctgtggaac ctgggcaacg aacccgacct gttcgcctgg ccgcccaacg ccgccgccgg
     4321 ccgggagtgg gtgcggcgca tgaccggcct cattaaggag cttgacccca ctcgtcccgt
     4381 cacctgcggc ctgcacgttg acagcgtctt ccgcgacaac ggcctgcgca tccacgatgt
     4441 cttcgccgaa gtcgatatcc ccgtcatgca cggctacccg atgtacatcg actggctcga
     4501 cgacccactc gacgtggact tcgtgcctta cctttgcgct ctgaccacgg cgctgtgcgg
     4561 caagccgaca ctgatggagg agttcggcgg ctgcaccaac agccccggtg agccatcggc
     4621 ctattgggag tggagcgcct acggcgagcc gcgccgccag ttcatggcct cagaagaggc
     4681 cctggccgac tatatcaccg ccgtgctgcc gcggctggtg gaggttgggg caacaggcgc
     4741 gctgctatgg tgctttgccg actacgtgcc ggagctatgg gatcggccgc cctgtgagca
     4801 gtcgatccac gagcgcttct tcggccttgt ccgtcccgac ggcacagtca agccccatgc
     4861 tcttgccctg caagcctttg ccaacacgcg gccgaccgtc cagccggcga cacggccatt
     4921 gaccctcgac atctcgccgg aggagtacta ccgcgacccg gccggccacg cggcgcggct
     4981 ttatcgcgtc ttccgcggcc agagttaagc gccagcctgc ccaaagcacg ctcgtctcga
     5041 ccattgaaag gccctggtcg ggcgcgcctc gcggctgaca actgctgaat gacggtgtaa
     5101 actagcgccc catgcctcgc aagaaaatcc tcttcatcgg cggctcgcta aatatgacgc
     5161 ggatgaccca tgctgtcgcg cgccatctgg ccgatgaatt tgagtgctgg ttcagcccgt
     5221 tctactccga cgggctatac gacatcctgg ccagccacag ccacctactc gacttcaccg
     5281 tgctgggcgg caacttccgc cgccagaccg acgcctacct gcgccagcac aacctgccta
     5341 tcgactaccg cggcgcggcg cgcgaatatg acctctacgt ggccacaacc gacctctatg
     5401 tgcccaagaa cctgcgacgg cggccgttta ttctcatcca ggagggcatg accgacccgg
     5461 aaggctttat gtaccacatg gtcaagtggt ttcgcctgcc gcgctacctg gccagcacat
     5521 cgaccaatgg cctgtcgcac caataccagt acttctgcgt cgcctcagag ggctatcgcg
     5581 actttttcat tcgcaagggc gtgcggccgg agaagctgat cgtcaccggc attcccaact
     5641 tcgacaacat cgccaccaac cttgacaacg acttccctca acgcggctat gtcctggtgg
     5701 ccacgtccga cgcccgcgag acctacaagt tcgacaaccg ccgcaagttc ctgcgccagg
     5761 cggtggacat cgccgccggc cggccgatga tcttcaagct gcaccccaat gagaacttca
     5821 agcgggccac gcgcgagatc cgcgaggtcg tcggcgacgt gcccatctac accgacggca
     5881 acatcgacca catgatcgcc aactgcgaca cgctcatctg ccagtactcg actgtcgtct
     5941 acgtcggcat cgccctgggc aaggaggtct actcctactt cgacgtggac acgctgcggc
     6001 ggctgacgcc cctgcagaac ggcggccaat cggggcgcaa catcgcccag gtctgccgcc
     6061 acatcctgct gggcgagccg gtcaattggg aggagttcct tggccaggct gtctagcccc
     6121 ggcggcgcgc cgccccaccg ggtcgtcacc gtcgttcagg cgcgcaccgg ctcgacacgg
     6181 ctgcccaaca aggtgctgcg gccactggcc ggctcgccgc tgctggtgcg catggtcgag
     6241 cgcgtccgcg ccgcccgcct cgccggcacg gtcgtcgtcg ccaccacgac agcgccggac
     6301 gatgacccca ttgccgacct ctgccgcgcc gagggctggc cctgcttccg cggcgacccg
     6361 ctagacctgc tcgaccgcca ctaccaggcc gggctggcct atggagccac ggccgtggcc
     6421 aagattccgt ccgattgccc gctcatcgac ccggcgatca tcgaccgcgt gctcggccac
     6481 ttcctcgatc accccggcgc gttcgacttc gtcagcaacc tgcacccggc cagctacccc
     6541 gatggcaacg atgtcgagat catgacgctc gacgccctgg ccaccgcctg gcgcgaggcc
     6601 acccggccgc tggagcgcga acacaccacg ccctatctgt gggaaaatcc cgaccgcttc
     6661 cgcctcggca acgtcctctg ggagacgggg ctggactact ccaaatccca ccgctggacc
     6721 atcgactacg aggaagacta ccagttcatc cgcgccgtct acgaggccct ctacccggcc
     6781 aacccgcgct tcggcctggg cgacatcctg gcctacgggg agagccaccc ccacgtggcc
     6841 gcgctcaaca gcgcctacgc gggggagtac tggtatcgcc accatcccgg cgaactgaag
     6901 accgtttcgg ggtagcctgc caccggcggc cgcccggctt aatttccaac aaggcaaagc
     6961 tccatgactc aagaagaaat cgaacaactg caagagatgg ctctgcgcgt gcgcgagcac
     7021 atcatccgcc tgtccaccga cggcggctgt ttcaccggcg cgtcgctgtc gtgcgccgat
     7081 ctgatcgtct acctctacaa ccgctttctc aatgtcggcc cggacaactt cgacgcgccc
     7141 gaccgcgact acctcttcct gtcaaagggc cacgacgtgc ccgcgctcta tgggacgttc
     7201 gtcgagctgg gctggctggc ggccgagcgg ctgggcaacc acctgaagac caacgacttc
     7261 atctactggc atcccaaccg ggccatcccc ggcatcgagt tccactccgg ctcgctgggt
     7321 catctgccgt cggtggccgt cggcgtggcc ctggactgca agctg
//
LOCUS       DAOSMQ010000102         7234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10352_length_7234_cov_4.567489, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000102 DAOSMQ010000000
VERSION     DAOSMQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10352_length_7234_cov_4.567489"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..552)
                     /locus_tag="PK829_04900"
     CDS             complement(163..552)
                     /locus_tag="PK829_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020335491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40585.1"
                     /translation="MKARIRPPRRAAGGSGRARVNWNTVLLEQIRLAGLPEPLREYVF
                     HANRNWRFDFCWRGDGLLVACEIEGGIWMQTDTGRSKGHAHPERFEQDCEKYNEAALY
                     GWTLLRVTPDMIRDGRAIDWLDRALRT"
     gene            complement(677..1348)
                     /gene="rpe"
                     /locus_tag="PK829_04905"
     CDS             complement(677..1348)
                     /gene="rpe"
                     /locus_tag="PK829_04905"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU40586.1"
                     /translation="MPPIHLAPSILSADFARLGAHVEEALDAGADFIHVDVMDGHFVP
                     NITIGPLIVEAVRPLADRRGAVVDVHLMIARPERYLADFARAGADILTVHVETCPHLH
                     RTVQAIRELGVRPGVTLNPATSLTTLDEILPYVDLVLVMSVNPGFGGQSYIPTSTAKI
                     ARLRAMLDAIGSTAELEVDGGIKPGNAAEVVAAGATVLVAGSAIFGGALSVAENVAAF
                     QAALA"
     gene            complement(1351..2334)
                     /gene="glpX"
                     /locus_tag="PK829_04910"
     CDS             complement(1351..2334)
                     /gene="glpX"
                     /locus_tag="PK829_04910"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018017929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOU40587.1"
                     /translation="MSEQPERNLGLDLARATEAAALAAARWVGRGQKNEGDGAAVNAL
                     RLLLGTVRMDGIVVIGEGEKDEAPMLYNGERVGNGHGPAVDVAVDPVEGTNLLAKGRP
                     NSIAVVAVAPRGTMFNPGPAFYMDKLIVGPEAAGQVDLEAPVAETLAIIAAAKGLRVD
                     EVTVVILDRPRNQARIQAVRDAGARIRLITDGDVAPSLMAALPGTGIDAVMGIGGTPE
                     GVITACAMHGLGGEIQGCLAPQSDAERARIEAAGIAIGRKLTLVELAAGDDHFFAATG
                     ITQGDFLRGVRYTATGAITHSLVTRSRSGTWRTIEAHHRWDKLNRISAVEY"
     gene            2544..4589
                     /locus_tag="PK829_04915"
     CDS             2544..4589
                     /locus_tag="PK829_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU40588.1"
                     /translation="MPILSATDLHHAYGADDLFDEVAVAVEARDRIALVGPNGVGKTT
                     LLLALAGLLEPSGGTVEQAVGLTLGYLRQEAALTFAGRENTVYGEMLTVFAELHRREE
                     EMAALEAALVEAYSPELLETYGTAQAAFEAAGGYQYHNDIKRVLQGLGFPPEEWDTPL
                     LHLSGGQKTRVLLGRLLLEQPALLIMDEPTNHLDIAAIEWLEGTLRRWEGALILVSHD
                     RYFLNRVVNRVWELAPESGGPARLRAYRGNYDAYVRQRQDAWERAQRLYEEEKERLAA
                     EADFIQRHIAGGQTDIAKGRLRQLTRDLALIDQIGLAAMAEMRRGNRGWIEVGTRART
                     LTINEAVERIRALRPPGARPPRLNIRLETTARSSRVVLRAKDATIGYPGRPLFGVHRL
                     KLERGGRAALLGPNGSGKSTFLRTVLGDIPPLAGEVALGDGVQVGYFAQAHDQLVPER
                     RVIDEIWARSRLNETEARGYLAHYLFRGDDVFKRVRELSGGERGRLALALLALEGANF
                     LLLDEPTNHLDIPSQEVLQEVLEAFNGTILLVSHDRYLVDRLAQHIWSIEEGELLAFT
                     ATYQEFLAEREGGAPPAESEPEAAPEPPAPVAAAEEAQPTAPAAGWSRQARHREERRR
                     RALATELEDAEYWLARTAEALEEARAAGDAAAPELEAEHTAARARLDELLAEWELLA"
     gene            4586..5281
                     /locus_tag="PK829_04920"
     CDS             4586..5281
                     /locus_tag="PK829_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU40589.1"
                     /translation="MTSTSAPPFRGVIFDFNGVLFWDNPLHEEAWRRTSRQLRGRPLT
                     DHEMTRQVHGKVNRDIFTYVLGETPGDEQLATLIAAKEAIYRQLCLAADGVFRLSPGA
                     AELLDFLVAAGIPHAIATSSPHQNLVFYVEHLGLHRWFSDDHLIYDRGAYPGKPAPGI
                     YLEAAARLGLPPPACVVVEDSIAGIAAARAAGIGHIVALGPADGHAELASLPDVATVI
                     VDLGSFPRHLVAT"
     gene            5435..6202
                     /locus_tag="PK829_04925"
     CDS             5435..6202
                     /locus_tag="PK829_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40590.1"
                     /translation="MKRLNTVLIAVLLVLALAACEDTTVHTTPPAPVSGDSSGGPTVT
                     IDPSSVGPNTSITVTGQGFPADTVVELSVKRPPESFTSEPLGQGQTDADGNVSFAAQV
                     PALWLDGTPLEGPELTLELTTEDGDIRAVATVPFTAEELKSFLAVTPSSGAPGQEIQL
                     QGIGFEPGQAFVVRVGPTATELVEDTLAEDSVGADGNFQETFTLPTTWPDTGADITEP
                     SLVFAVVNADSGDIMATVSFSNDPAQAVPEGMDTPVP"
     gene            6364..6978
                     /gene="amrA"
                     /locus_tag="PK829_04930"
     CDS             6364..6978
                     /gene="amrA"
                     /locus_tag="PK829_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOU40591.1"
                     /translation="MNTHEPHRLTTDEERAELLGLARRAIREAIATGDPLAYETDNPY
                     FLKPAAVFVTLRSAPYYYEPEGELRGCVGRTDADAPLYLAVQDAAIKAATIDPRFYPV
                     TTDELDQLSIEISILSPMRPVTHYDDIEIGRDGLLIIGNHRRGLLLPEVPTHFGWDVR
                     EFVRQLCRKASLPDDAWPDRAQLLAFTTESFTAPGQSDRSRAER"
     gene            complement(6975..>7234)
                     /locus_tag="PK829_04935"
     CDS             complement(6975..>7234)
                     /locus_tag="PK829_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40592.1"
                     /translation="EVELNNDQTRTIYFFAKDRREGVLDSVPSGWQVMEGRNALPVLS
                     KEDSERRNGSGRSSGNGRSSSKRSNSNSGSKRSSSSRSGND"
BASE COUNT         1159 a         2368 c         2540 g         1167 t
ORIGIN      
        1 tgacgtggat gaggtcgccc tggtgttcga ccagacggcg agcggcacgc tgttcctcgc
       61 cgatgtcgct gtggagaggt aggagattta acgccaggac gcaaagacgc caggaagaag
      121 tcttctggcg tctttgcatc ttggcgtctt ggcgttgatc tcttacgtcc gcagggcgcg
      181 gtcgagccag tcgatggctc ggccgtcgcg gatcatgtcc ggcgtgacgc ggaggagcgt
      241 ccagccgtag agggcggcct cgttgtactt ctcgcaatcc tgctcaaagc gttcggggtg
      301 ggcgtgaccc ttgctgcggc cggtgtcggt ctgcatccag atgccgcctt cgatctcaca
      361 ggccaccagc aggccgtcgc cccgccagca gaagtcgaag cgccagttgc ggttggcgtg
      421 gaagacgtac tcgcggagcg gctccggcag cccggccagg cgtatttgct ccaggaggac
      481 ggtattccag ttgacgcggg cgcggccgga gccaccggcg gcgcgtcgcg gcgggcggat
      541 gcgagccttc atggataaaa gatagcacag atgttcgagg ttgccaaggg cacgcggccg
      601 gtgcgtcgca ctttcttggg gagggggtta agaaagtgcc tggcactcag gaaagtgcca
      661 ggcactggga ggcaggttag gccagggcgg cctggaacgc ggcgacattc tccgccacac
      721 tcagcgcgcc gccaaagatc gcgctgccgg ccaccaacac cgtcgccccg gcggcgacca
      781 cctcggcggc gttgccgggc ttgatgccgc catcgacctc cagctcggcc gtcgagccga
      841 tggcgtccag catggcccgc agccgggcga tcttggccgt gctggtgggg atgtacgact
      901 ggccgccgaa gccggggttg accgacatga ccaggacgag atcgacgtag ggcaggattt
      961 cgtccagcgt tgtcagcgag gtggccgggt tgagcgtcac gcccggccgc acgcccagtt
     1021 cgcggatggc ctgtacggtg cggtgaaggt gggggcaggt ctccacgtgg acggtcagga
     1081 tgtcggcccc ggcgcgggcg aagtcggcca gatagcgctc tggccgcgcg atcatcaggt
     1141 ggacatcgac gaccgccccg cgccggtcag ccaacggccg gacggcctcg acgatgagcg
     1201 ggccgatggt gatgttgggc acgaagtgcc cgtccatcac gtcgacgtgg atgaagtcgg
     1261 ctccggcgtc cagcgcctct tccacgtgcg cgcccaggcg ggcgaagtcg gccgagagaa
     1321 tggacggagc caggtgaatg gggggcatgg ctaatactcc accgcgctga tgcggttcag
     1381 cttgtcccag cggtgatggg cttcgatggt gcgccacgtg ccggagcgcg agcgcgtgac
     1441 gagcgagtgg gtgatcgcgc cggtggcggt gtagcggacg ccgcgcagga agtcgccctg
     1501 ggtgatgccg gtggcggcga agaagtggtc gtcgccggcg gccagctcga ccagggtcag
     1561 cttgcggccg atggcgatgc cggcggcctc gatgcgcgcc cgctcggcgt cggactgcgg
     1621 cgcgaggcag ccctggattt cgccgcccag cccgtgcatg gcgcaggcgg tgatgacccc
     1681 ttccggcgtg ccgccgatgc ccatgacggc gtcgatgccc gtgcccggca gcgcggccat
     1741 cagcgagggg gccacgtcgc cgtcggtgat cagccggatg cgcgcgccgg cgtcgcggac
     1801 ggcctggatg cgggcctggt tgcgcggccg gtcgaggatg acgacagtga cttcatcgac
     1861 ccgcagccct ttggctgcgg cgatgatggc cagcgtctcg gccacgggcg cttcgagatc
     1921 gacctggccg gccgcctctg gcccgacgat gagcttgtcc atgtagaagg ccgggccggg
     1981 gttgaacatt gtgccgcgcg gggcgacggc gacgacggcg atggagttcg gccgtccctt
     2041 ggccagcagg tttgtgccct ccacgggatc gacggcgaca tcgacggccg ggccgtggcc
     2101 gttgccgacg cgctcgccgt tgtagagcat cggcgcttcg tccttttcgc cctcgccgat
     2161 gacgacgatg ccgtccatgc gcaccgtgcc caggagcagg cgcagggcgt tgacggccgc
     2221 gccgtcgccc tcgttcttct gcccgcggcc gacccagcgc gccgcggcca gggcggccgc
     2281 ctccgtcgcc cgcgccagat cgagtcccag gttgcgttcc ggttgttcgg acatgacttg
     2341 cctcctgtgg gctttgtgaa agacggaaca gggacagtat agctgcaaga agggatgggg
     2401 actagggatt aggggctagg gagcaggttc caggggccag gggccagggg ccagggagaa
     2461 gagcactctt ccctggaacc tggcccctgg aacccatcct ccagaactgg cccccgaccg
     2521 cgtccccggc tagaatgacc gctatgccca tcctgagcgc cacagacctg caccacgcct
     2581 atggagccga tgacctgttc gatgaggtcg ccgtggccgt cgaggcgcgc gaccgcatcg
     2641 ctctggtggg gccgaacggc gtgggcaaga cgacgttact gctggccctg gccgggctgc
     2701 tggagccgag cggcggcacg gtggagcagg ctgtcgggct gacgctgggc tatctgcggc
     2761 aggaggcggc gctgaccttc gccggccgcg agaacactgt ctacggcgag atgctgaccg
     2821 tctttgctga gttgcaccgc cgcgaggagg aaatggcggc gctggaggca gcgctggtcg
     2881 aggcctattc gcccgaactg ctggaaacct atggcacggc gcaggccgcc ttcgaggccg
     2941 ccggcggcta ccagtaccac aacgacatca agcgcgtgct gcaagggctg ggctttccgc
     3001 cggaggagtg ggacacgccg ctgctgcacc tgagcggcgg gcagaagacg cgcgtgctgc
     3061 tcggccgctt gctgctggag cagccggcct tgctgattat ggatgagccg accaaccacc
     3121 tcgacatcgc cgccatcgag tggctggagg gcacgctgcg ccgctgggaa ggggcgctca
     3181 tcctggtcag ccacgaccgc tacttcctca accgcgtcgt caaccgcgtc tgggaactgg
     3241 ccccggagag cggcgggccg gccaggctgc gcgcttaccg cggcaactac gacgcctatg
     3301 tccgccagcg ccaggacgcc tgggagcggg cgcaacggct gtatgaggag gagaaggagc
     3361 ggctggcggc cgaggccgac ttcatccagc gccatatcgc cggcggccag accgacatcg
     3421 ccaaggggcg gctgcggcaa ttgacgcgcg acctggccct catcgatcag atcgggctgg
     3481 cggcaatggc cgagatgcgc cggggcaacc gcggttggat cgaggtcggc acgcgcgcgc
     3541 gcacgctgac catcaacgag gccgtcgagc gtattcgcgc cctgcggccg cccggtgcgc
     3601 gcccgccacg gctcaacatt cgcctggaga cgaccgcgcg cagcagccgc gttgtgctgc
     3661 gggccaaaga cgcgaccatt ggctatccgg gccggccgct tttcggcgtc caccggctga
     3721 agctggagcg cggcggccgg gcggcgctgc tggggccgaa cggcagcggc aagagcacct
     3781 tcctgcgcac cgtgctgggc gatattccgc cgctggccgg cgaagtggcc ctcggcgacg
     3841 gcgtgcaggt tggctacttc gcccaggccc acgaccagct tgtgcccgag cggcgggtca
     3901 tcgacgaaat ctgggcgcgc agccggctga acgagaccga ggcgcggggc tatctggccc
     3961 actacctctt ccgcggcgac gacgttttca aacgggtgcg cgagttgagc ggcggcgagc
     4021 gcggccggct ggccctggcg ctgctggcgc tggagggcgc caacttcctg ctgctcgacg
     4081 agccgaccaa ccacctcgac attccctcgc aggaggtgtt gcaggaggtg ctggaggcgt
     4141 tcaacggcac catccttctc gtctcccacg atcgctatct ggtggatcgg ctggcgcagc
     4201 acatctggtc tatcgaggag ggcgagctgc tggccttcac ggccacgtat caggagttcc
     4261 tggccgagcg cgaggggggc gcgccgccgg ccgagagcga accggaggct gcgccggagc
     4321 cgcccgcgcc cgtcgccgcg gccgaggaag cccagccgac cgcgccggcc gccgggtgga
     4381 gccggcaggc gcgccaccgc gaggagcgcc gccgccgtgc cctggccacg gaactggaag
     4441 acgcggagta ctggctggcg cggacggccg aggcactgga agaggcccgc gccgccggcg
     4501 acgccgccgc gcccgaactg gaggccgagc acactgccgc gcgcgcgcgt ctggacgaac
     4561 tgttggccga atgggagcta ttggcatgac atcgaccagc gcgccgccct tccgcggcgt
     4621 cattttcgac ttcaacggcg tgctgttctg ggacaacccg ctgcacgagg aagcctggcg
     4681 gcgcacctcg cgccaactgc gcggacgccc tctgaccgac cacgagatga ccaggcaggt
     4741 tcacggcaag gtcaaccgcg acatcttcac ctacgttctg ggcgagacgc ccggcgacga
     4801 gcagttggcg acgctgatcg ccgcgaaaga ggccatctac cggcagctat gcctggcggc
     4861 ggacggcgtc ttccggctct ctccgggcgc tgctgaactg ctggacttcc tggtggccgc
     4921 gggcatcccc cacgccatcg ccacgtcgtc gccccatcag aaccttgtct tctacgtgga
     4981 gcaccttggt ctacaccgtt ggttcagcga cgatcacctc atctacgacc gcggcgccta
     5041 tcccggtaag cccgcgccgg gcatctacct ggaagcggcg gcgcggctgg ggctgccgcc
     5101 gccggcctgc gtcgtcgtcg aggactccat cgccggcatc gccgcggcgc gagcagcggg
     5161 gatcggccac atcgtcgccc tcggcccggc cgacgggcac gccgaattgg cctctctgcc
     5221 ggacgtggcc acagtgatcg tcgacctggg cagcttcccg cgccatctgg ttgcgacgta
     5281 atgacgcccg cgcggcgcgg cggtaggcta tttgacggcc tccggcagcc ctgaaaaggg
     5341 tcattcaacc gttgggcagg gtgggccggc tgcctatcct ttgtccacgc ggcggggttg
     5401 tctgagtcgc ccgccccgag gagaaggaaa agtcatgaaa cgcctgaaca ccgtcctcat
     5461 tgctgtgctc ctggtgctgg ctctggctgc ctgcgaggat acgaccgtcc acaccacgcc
     5521 gcccgcgccc gtcagtggcg acagcagcgg cgggccaacc gtcaccatcg acccgtcctc
     5581 ggttgggcca aatacgtcca tcactgtcac cggccagggc ttcccggccg acactgtggt
     5641 cgagctgagc gtcaagcggc cgccagagag tttcacttct gagccgctgg ggcaggggca
     5701 gaccgacgcc gacggcaacg tgagcttcgc cgctcaggtg ccggcgctgt ggctcgacgg
     5761 cacgccgctg gaggggccgg agctgaccct ggaactgacg accgaggacg gcgacatccg
     5821 cgccgtggcg acggtaccct ttaccgctga ggaattgaaa tcgtttctgg cggtgacgcc
     5881 cagcagcggc gcgccgggcc aggagatcca gctacagggc attggctttg agccgggaca
     5941 ggcgtttgtc gtgcgcgtcg ggccgacggc aaccgagttg gttgaggaca cgttggccga
     6001 ggacagcgtt ggcgcggacg gcaacttcca ggagacgttc acgctgccga cgacgtggcc
     6061 ggacacgggc gcggacatca ccgagccatc gctcgtcttt gccgtggtca acgccgacag
     6121 cggtgacatc atggcgacgg tgtcgttcag caacgacccc gcccaggcgg tgccggaggg
     6181 aatggacacg ccggtgccct agaaccaacg cggccaagac ggacgggcaa ttgacaaaag
     6241 cggcctattc aggccgcttt tgcgtgtagt tgttaccctg ccggtcagcc cttcaggtgg
     6301 ggggcaagga ctggggaata tccgaacaat ctattgtata ctgaggggaa gggagtgatg
     6361 acaatgaaca cccacgaacc gcaccgcctg accacggacg aagagcgcgc cgaactgctg
     6421 ggcctggccc gccgcgccat ccgcgaggcg atcgctacag gcgatccgct ggcttatgaa
     6481 accgacaacc cgtatttcct aaagccggcg gccgtcttcg tgaccctgcg cagcgcgccg
     6541 tattactacg agcctgaggg ggagttgcgc ggctgcgtcg gccgcacaga tgcggacgcg
     6601 ccgctctatc tggcagtaca ggacgcggcc attaaagcgg cgaccatcga cccgcgcttc
     6661 tatcccgtca caaccgacga actagaccag ttgagcattg agatttcaat cctttccccc
     6721 atgcggcctg tcacgcacta tgacgacatt gaaataggcc gcgatgggtt gctcatcatt
     6781 ggcaatcacc gtcgcggcct gttattgcca gaagtgccaa cgcacttcgg ttgggatgtg
     6841 cgggagttcg tgcggcaact ctgtcggaag gcgtcgctgc cggacgatgc ctggccagat
     6901 cgggcgcaac tactggcctt tacgaccgaa agttttaccg cgcccggcca atccgatcgt
     6961 tctcgcgctg agcgctagtc gttgccgctt ctgctgctgc ttgagcgctt actgccgctg
     7021 ttgctgttgg agcgcttgct gctgctcctg ccgttgccac tactgcggcc gctgccgttg
     7081 cgtcgctccg aatcttcttt ggagagaacc ggtagcgcgt tacgtccttc catcacctgc
     7141 caaccggagg gcacgctatc gagcacaccc tcgcgccggt ccttggcgaa gaagtagatc
     7201 gtgcgcgttt gatcgttgtt gagttcgact tcac
//
LOCUS       DAOSMQ010000103         7191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10412_length_7191_cov_4.580297, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000103 DAOSMQ010000000
VERSION     DAOSMQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10412_length_7191_cov_4.580297"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PK829_04940"
     CDS             complement(<1..1070)
                     /locus_tag="PK829_04940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU40593.1"
                     /translation="MKELSRVVSSIPPSETLAVSDRAKALKAAGQDVVALAGGDPDFD
                     TPDYITAAAFQAIENGATHYPAPMKGITPLLEAIAEKMATDNGITVKPGSDIVVTPGG
                     KWALFLALSAILNPGDEVLYLEPVWVSYPPMIALAGGTPVAVTLPSEDNFRITADRLR
                     AKLTPRTKALMVNSPNNPTGRVLTAEELDDIAAVALENDLYVISDEIYETIIFDGRKH
                     LSPGARPGMAERTITINGLSKSHAMTGWRLGWLAAPTPIAKLATQMNSQTVSSAANFT
                     MHAAVAALRGPKDTTRAMRDEYQQRRDFMVPALNAIDGVECANIEGAFYLFPRFPNSR
                     RSSAELAETLIDKAGIASVPGGA"
     assembly_gap    1092..1191
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1192..1587)
                     /locus_tag="PK829_04945"
     CDS             complement(<1192..1587)
                     /locus_tag="PK829_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU40594.1"
                     /translation="MDWFQREIVLTPRPRGFHLITREVVAGVPEIGRFAAGLAHFFLR
                     HTSAGLALNENADPSVRADMAAYFNRLVPENAPYFTHTAEGPDDMPAHLKAVLLGSEL
                     TIPISGGRLALGTWQGIYLCEHRDDGGPRR"
     gene            complement(1737..2909)
                     /locus_tag="PK829_04950"
     CDS             complement(1737..2909)
                     /locus_tag="PK829_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40595.1"
                     /translation="MLRRIWIIIRKEILDNLRDRRSVGNPLLYVVLFGFLNRTFTEQA
                     ERPLALHVLGAANAPNLVQFLDQNNVDILEAPADAEDAVRRGDLAVVLVIPEEFGEQF
                     TRGQPAQVRLLVDDSNQSGSIAAGRAQSLINQYSSQIGALRLIARGVSPAISSAVPVE
                     WVSTTAGNVGANTASVVLNLLPVVMMTAVFYGGFYLAVDATAGERERKSLEPLLLNPV
                     PRREFVIGKFLAVFAFTLLATFLATALFLVLLGIPQIQNFTNIQLNVGWSVILAAVGL
                     IIPVAFMAVALEMLVASNARSVKEAQTYTQLIAFAGFLPSLFLSVLPIRPQEWMYLIP
                     TIGQLYFITDMSRGLPLDAAQVVLCSLLTAAIGAAALLATMRLYNREQIILGKSSA"
     gene            complement(2909..3640)
                     /locus_tag="PK829_04955"
     CDS             complement(2909..3640)
                     /locus_tag="PK829_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU40596.1"
                     /translation="MIEVTQLTKSFGPVKAVQDVSFNAPDGLITGLLGPNGAGKSTTM
                     RLIAGALQPDTGQTAIDGFDTQAQRLEAQRHLGVLTDAHGLYQRLTARENIRYFGQLR
                     DLGGKALEQRIDALIALLEMSDIADRRTEGFSTGEQGKVAIARALVHQPQNVMLDEPT
                     AGLDVMSTRAMRGVIRQLREEGRCILFSSHIMQEVAMLCDNIVVIAGGRVVAQGSPDD
                     LRQRTGQENLEDAFVAIIGTKEGLV"
     gene            complement(3657..5123)
                     /locus_tag="PK829_04960"
     CDS             complement(3657..5123)
                     /locus_tag="PK829_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU40597.1"
                     /translation="MKRIACLLFLLSLLLIACSGRTPEDERQPTIPLTACRLSGGVDA
                     QCGQLLVYEDRAAAAGRQISLNIAVLPATGSSNVVADDPLFLLAGGPGQAATETYPLA
                     ASLFADVNRNRDIVLVDQRGTGESNGLNCAVLEDETLPADLPDDEQIALLDQCRLDLG
                     ERADLSLYTTDAFVADLDDVRAALDYDTINLYGASYGTRAALTYMRRFPERVRSVVLD
                     SVVGPELVLFLQMPQDGQRALDLLFDRCAADDACNEAFPNFRAEYEAVLARLATPQPV
                     TVADPLSNKPLAFQLDRDFLSQLVFNSLYSAEFQGLLPLLIHHAHETGDYAPLIVQGL
                     AITSSASLYPGLLYAVTCSEDAPLIDPAEAESIRAGTSFGDFAARFQTICANWPRADI
                     AADFRDPLQSDIPTLLLSGGADPVTPPEYAAQVAAGLSNSRHIVVPGFGHGVIGLGCM
                     PSLVAEFVRATDPAALDATCLDVLQPPPFFVSFAGPQP"
     gene            5385..6956
                     /locus_tag="PK829_04965"
     CDS             5385..6956
                     /locus_tag="PK829_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HOU40598.1"
                     /translation="MTKGHFDTVGRSPRLPGSRLKSLLVATFFLVLVLGGPLVARGSN
                     DLFLPRAARSGAPSVPALRSRAVDINWAALSPDTNELRLNPFEGLTLAATRLRVDRPV
                     GGGYVWVGRLDGQAGSYVTLSVLNGVVAGSVFRQGREWLVIRPNPAGGHMVYELDPAA
                     REPGGSDVVFPPAGAALPLAEAPAAAVCEDDGTVIDVLVAYTATASAENGGNDALTAL
                     INQRISDMNTANEFSAAPFTWRLVGTLAVDYAESGDLSIDLAALTAPADGLLDAVHAA
                     RDAARADLVALLVAQGNGGKCGLAYQMMALDSAFASQAFGVTALDYPGDYVCGGLTLA
                     HEMGHNLGNAHDRAHHTGGVLFPYSYGYQAPSEAFRDIMSYDCTDGCPRLNFWANPDV
                     WYLGEPTGVDYETDPANAADIVRSMNGARLTAANFRNTCALPTATATPSPTPTDPPTA
                     TPSPTTTPSPTMTSTPTATPTWTPTPTMTGTPTRGPTPTITATPRPTRRPTRTPRPSP
                     TPEAWRAFAPAIVGR"
     gene            complement(6964..>7191)
                     /locus_tag="PK829_04970"
     CDS             complement(6964..>7191)
                     /locus_tag="PK829_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40599.1"
                     /translation="AEAAAAAMGMDPLRRQLQAATAISLRALGRNAEAEQQQAAARAT
                     ANAIAARIGDETLRREYLSAIEGRQLLLAQP"
BASE COUNT         1179 a         2293 c         2467 g         1152 t
ORIGIN      
        1 gcgccgccgg gcacggaggc gatgccggcc ttgtcgatga gcgtctcggc cagttcggcg
       61 ctgctgcggc ggctgttggg gaagcgcggg aacaggtaga acgcgccctc gatgttggcg
      121 cactccacgc cgtcgatggc gttcagcgcc ggcaccatga agtcgcgccg ctgctggtat
      181 tcgtcacgca tggcccgcgt cgtatccttc ggccctctca gcgcggccac ggcggcatgc
      241 atggtgaagt tggccgccga ggagacggtc tggctgttca tctgcgtggc cagtttggcg
      301 atgggcgtcg gcgcggccag ccagcccagc cgccagccag tcatggcgtg ggacttcgac
      361 agaccgttga tggtgatcgt ccgctcggcc atgcccggcc gcgcccccgg cgagaggtgc
      421 ttgcggccgt cgaagatgat cgtctcgtag atctcgtcgg agatgacgta gagatcattc
      481 tccagagcga cggcggcgat gtcgtccagt tcctcggccg ttagcacgcg gccggtgggg
      541 ttgttgggcg agttgaccat cagcgccttg gtgcgtggcg tcagcttggc ccgcagccgg
      601 tcggcggtga tgcggaagtt gtcttcgctg ggcagggtca cggccacggg tgtccccccg
      661 gccagagcga tcatcggcgg gtaggacacc cataccggct ccaggtagag cacctcgtcg
      721 ccggggttga ggatggccga cagggccagg aacagcgccc acttgccgcc gggcgtcacc
      781 acgatgtcgc tgcccggctt gacggtgatg ccgttgtcgg tggccatctt ctcggcgatg
      841 gcctccagca ggggcgtaat acccttcatc ggcgcggggt aatgggtcgc gccattttca
      901 atcgcctgaa acgcggcggc ggtgatgtag tcgggcgtgt cgaaatcggg gtcgccgccg
      961 gccagggcga caacgtcttg cccggccgct ttaagggcct tggcccgatc gctaaccgcc
     1021 agggtttcgg agggggggat gctactgacg acgcgggata gttctttcat gggggcggat
     1081 tctagcagaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgccgcgg
     1201 cccgccgtcg tcgcggtgct cgcacaggta gatgccctgc cacgtgccca gcgccagccg
     1261 gccgccgctg atagggatgg tcagttcgct gcccaggagc acggccttca ggtgggcggg
     1321 catgtcgtcg gggccttcgg cggtgtgggt gaagtagggc gcgttctccg ggaccaggcg
     1381 gttgaagtag gccgccatgt cggcgcggac actggggtcg gcgttctcgt tgagggccag
     1441 cccggccgaa gtgtggcgca gaaagaagtg ggccaggcca gcggcgaagc ggccgatctc
     1501 cggcacaccg gccacgacct cgcgggtgat caggtggaag ccgcgcgggc ggggagtgag
     1561 gacgatctcc ctttggaacc agtccacggt caataacctc gcagctgtcg ttgctttatc
     1621 gcttacccgc gctcagaata gtgacgccgc atacggattg cgattgtaga aggcgcagtg
     1681 cggaatacca agttagtcat aggccctcgc gcgattcggg agaatcgcgc cacgccctat
     1741 gccgacgact tgcccaaaat gatctgctcc cggttataca gccgcatcgt cgccagcaac
     1801 gccgccgcgc cgatggccgc cgtcagcagg ctgcacagca ccacctgcgc cgcgtccagc
     1861 ggcaggccgc gcgacatgtc ggtgatgaag tacagctggc cgatggtggg aatcaggtac
     1921 atccactcct gcggccggat gggcagcact gacaggaaca gcgacggcag gaagccggca
     1981 aaggcgatca gttgggtata ggtctgggct tccttcaccg agcgggcatt cgaggccacc
     2041 agcatctcca gggccacggc catgaaagcc acggggatga tcaggcccac ggcggccagg
     2101 atgacgctcc agccgacgtt gagctggatg ttggtgaaat tctgtatctg agggatgccg
     2161 agtagcacca gaaagagggc cgtcgccaga aaggtcgcca gcaacgtgaa ggcgaagaca
     2221 gccaggaact tgccgatgac gaactcgcgg cgcggcacgg ggttgagcag cagcggctcc
     2281 agcgacttgc gctcacgctc gccggcggtg gcgtctaccg ccaggtagaa gccgccatag
     2341 aagacggccg tcatcatgac cactggcagc aggttgagca cgaccgaggc cgtattggcg
     2401 ccgacgttgc ccgctgtggt gctgacccat tcgaccggca ccgcgctact gatggccggg
     2461 ctgacgccgc gggcgatcag gcgcaacgcg ccgatctggc tgctatactg attgatgagc
     2521 gattgcgccc gcccggcggc aatactgccg ctctggttag agtcatcgac cagcagccgc
     2581 acctgcgccg gctggccacg ggtgaactgc tcgccaaact cctccgggat gaccaggacg
     2641 acagccagat cgccgcgccg cacggcgtct tcagcatcgg ccggcgcttc caggatatcg
     2701 acgttgttct ggtcgaggaa ctggacgagg ttgggagcat tggccgcgcc gaggacgtgc
     2761 agcgccagcg gtcgctccgc ctgctcggta aacgtccggt tcagaaagcc gaaaaggacg
     2821 acatagagca gcgggttgcc caccgagcgg cgatcgcgca ggttgtcgag gatctccttg
     2881 cgaatgatga tccaaatgcg ccgcaacatc agaccaggcc ctccttcgtg ccgatgatgg
     2941 ccacgaatgc gtcttccagg ttctcctggc cggtgcgctg gcgcaggtcg tcgggcgacc
     3001 cctgggcgac cacgcgaccg ccagcgatga cgacgatgtt atcgcacagc atggccactt
     3061 cctgcatgat gtggctgcta aagagaatgc agcgcccctc ctcgcgcagt tgccggatca
     3121 caccgcgcat ggcccgcgtg ctcatcacgt ctagcccggc cgtcggctcg tcgagcatga
     3181 cgttctgtgg ctggtggaca agggcgcggg cgatggcgac cttgccctgt tcgccggtag
     3241 agaagccctc ggtccgccgg tcggcgatgt cggacatctc cagcagggcg atcagcgcgt
     3301 caatgcgctg ctccagcgcc ttgccaccca ggtcgcgcaa ctggccgaag tagcggatgt
     3361 tctcgcgggc cgtcaggcgt tggtagaggc cgtgggcgtc cgtcagcacg ccgagatggc
     3421 gctgcgcctc cagccgctgc gcctgggtat cgaagccatc aatggccgtt tggcctgtgt
     3481 cgggctgcaa cgccccggcg atgaggcgca tcgtcgtgct cttgcccgcg ccgttggggc
     3541 cgagcaggcc ggtgatcagg ccgtcggggg cgttaaagga aacgtcctgg acggccttga
     3601 ccgggccgaa ggatttggtg agctgggtga cttcgatcat gatcttgcct ggcaagctag
     3661 ggctgcggtc cggcaaacga gacgaagaac ggcggcggtt gcaggacatc aaggcaggtg
     3721 gcgtcgagcg cggccgggtc agtggcgcgc acaaactcgg ccaccagact gggcatacag
     3781 cctagtccga tgaccccgtg gccgaagccg ggcacgacga tgtgacggct attgctcagc
     3841 ccggcggcga cctgcgcggc atactccggc ggcgtcaccg ggtcggcccc accggacagc
     3901 agcagcgtcg ggatgtcgct ttgcagcggg tcgcggaagt cggcggcgat gtccgcccgc
     3961 ggccagttgg cgcagatggt ctggaagcgc gcggcgaagt cgccgaagct cgttccggcg
     4021 cggatgctct cggcctcggc cgggtcgatc agcggcgcgt cttccgagca ggtgacggcg
     4081 tagagcaagc cgggataaag gctggcgctc gacgtgatgg ccaggccctg cacgatgagg
     4141 ggcgcatagt cgcccgtctc atgcgcatgg tggatcagca gcggcagcag cccctggaac
     4201 tcggcgctat agaggctgtt gaagaccagt tgcgagagaa agtcgcggtc tagctggaac
     4261 gccagcggct tgttcgacag cgggtcagcc acggtgaccg gctgcggcgt cgccagccgg
     4321 gccagcacgg cctcatactc ggcgcggaag ttggggaagg cttcgttgca ggcgtcgtcg
     4381 gcggcgcagc ggtcgaaaag caggtcgagc gcccgctggc cgtcctgagg catttgcagg
     4441 aaaagcacca gctccggccc gaccacggaa tcgaggacaa cgctgcggac gcgctccggg
     4501 aagcggcgca tgtaagtgag ggccgcgcgc gtgccgtagg acgcgccata gagattgatg
     4561 gtgtcatagt ccagcgcggc gcgcacgtcg tcgaggtcgg ccacgaaggc gtcggtcgtg
     4621 tagaggctca ggtcggcccg ctcgcccagg tcgaggcgac actggtcgag caaggcgatc
     4681 tgctcatcat cgggcaggtc ggccggcagg gtctcatctt ccagcacggc gcagttcagg
     4741 ccattcgatt cgcccgtgcc gcgctgatcg acaagaacga tgtcacggtt gcggttgacg
     4801 tcggcaaaca gcgacgcggc cagaggatac gtctcggtgg ccgcctggcc ggggccgccg
     4861 gccagcagaa agagaggatc atccgcgacg acgttgctgc tgcccgtggc cggcaaaacg
     4921 gcgatgttga gcgatatctg ccggccggcc gcggcggcgc gatcttcata gacgagcaac
     4981 tggccgcatt gggcatccac accgccgctc aggcgacagg ccgttaacgg aatcgttggc
     5041 tggcgctcgt cttctggcgt cctgccggaa caggcgatga gtaaaaggct aagcaggaat
     5101 aggagacagg caatgcgttt cattgggcag ctcggcggcc ggcgacacgc agatggcccc
     5161 ggtaccttct ggattgtaac atggcttgcg gcgcgccaag tcgcggcggg cgacctgaca
     5221 ggtaggctgc ctacctgtca ggtcgtcgtg ctttgtaaaa tgagagggag cctagcggtt
     5281 gctgagagcg cctatctctg acagtgtagc ctttcttgcg tggcggcggc gcgtggtaaa
     5341 cttgccgcgg ccaaaacgcc cggctcggag acgccagacc cgtcatgacc aaaggacatt
     5401 tcgacaccgt aggacgctcg cctcggctgc cggggagccg gctgaaaagc ctgctggttg
     5461 ccacgttctt tctcgttctc gtgctgggtg gcccgctcgt tgcgcggggc agcaacgact
     5521 tgttcctacc gcgggcagcg cggtcgggcg cgccatccgt ccctgccctg cgcagtcgcg
     5581 cggtggacat caactgggcc gctctgtcgc ccgacacgaa tgagcttcgg ctcaacccgt
     5641 tcgagggact gacgctggcg gcgacaaggc tgcgcgtcga ccggccggtt ggcggcggct
     5701 atgtgtgggt cggccggctg gacggccagg cgggcagcta cgtcaccctg tcggtgctaa
     5761 acggtgttgt agccggcagc gtcttccggc aggggcgcga gtggctggtc attcgcccca
     5821 acccggccgg agggcacatg gtttatgagc tggacccggc ggcgcgcgag ccgggcggct
     5881 ccgacgtggt tttcccgccg gccggcgcgg cgctcccgct tgcggaggcg ccggcggcgg
     5941 ccgtctgtga agacgacggc acggtgatcg atgtgctggt ggcctacacg gccacggcca
     6001 gcgccgagaa cggcggcaat gacgcgctga cggcgctcat caaccagcgt atcagcgaca
     6061 tgaacacggc caacgaattc agcgccgcgc cgttcacctg gcgattggta ggcacgctgg
     6121 cggtggacta tgccgagtcg ggcgacctat cgatcgatct ggccgccctg acggccccgg
     6181 ccgacggcct gctggatgcc gtccacgccg cgcgcgacgc cgcccgggcc gatctggtcg
     6241 ccctgctggt ggcccagggc aacggcggca agtgcggcct ggcctaccag atgatggcgc
     6301 tggacagcgc tttcgccagc caggcctttg gcgtgaccgc gcttgattat cccggcgact
     6361 acgtttgcgg tgggctgacg ttggcccacg agatgggcca caacctcggc aacgcccacg
     6421 accgagcgca ccacactggc ggcgtactct tcccctactc ctacggctac caagcgccca
     6481 gcgaggcttt ccgcgacatc atgtcctacg actgcaccga cggctgcccg cgcctcaact
     6541 tctgggccaa cccggacgtg tggtatctgg gcgagccgac gggcgtcgat tacgaaaccg
     6601 acccggccaa tgccgctgac attgtccgct cgatgaacgg ggccagactg acagcggcca
     6661 actttcgcaa cacgtgcgct ttgccaacgg cgacagcgac cccgtcgcca acgcccaccg
     6721 acccgcccac agcaacaccg tcgccgacaa cgaccccctc gcccacaatg acctccaccc
     6781 cgacggcgac gcctacctgg acgccgacgc ccacgatgac gggcacgccc acccgcgggc
     6841 caacgcccac catcaccgcc acgccgcgcc ccacgcgccg gccaacgcgc acgccgcggc
     6901 cgtcgcccac gccagaggcc tggcgcgcct ttgccccggc gattgttggt cgatagcgag
     6961 cggctacggc tgcgccagca gaagctgtcg cccctcgatg gcgctcaggt attcccggcg
     7021 cagcgtctcg tcgccgatgc gcgcggcgat ggcgttggcc gtagcccgcg ccgcggcttg
     7081 ctgctgctcg gcctcggcgt tgcggcccag ggcgcgcaga gatatagccg tcgccgcctg
     7141 aagttgccgc cgcagcgggt ccatgcccat cgccgcggcc gcggcctcgg c
//
LOCUS       DAOSMQ010000104         7186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10422_length_7186_cov_4.523489, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000104 DAOSMQ010000000
VERSION     DAOSMQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10422_length_7186_cov_4.523489"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1278)
                     /locus_tag="PK829_04975"
     CDS             complement(175..1278)
                     /locus_tag="PK829_04975"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HOU40600.1"
                     /translation="MKVKAAVFYEPGLPFAVETLDLAPPGAGEALVRVAAAGVCHSDW
                     HLMTGATRHATPVVPGHEGAGVVVAVGPGVARVAVGDHVALSWAPSCGACFYCLHNRP
                     SLCETYIAPLWAGTMLDGTTRLTQNGRPVYHFSALACFAEYTVVPEVCCVPLGADIPL
                     TVAALIGCAVTTGVGAALNTAQVRPGSSVVVFGAGGVGLSVIMGARLAGASRIIAVDA
                     HKAKGDMARAFGATDFLMAGPDTVRAIQNLTQGRGADTVFEAIGLPAVQEMCLAAARP
                     GGLVVLAGISPMGSATNLPGALITRQEKTVTGCYYGSANPARDFPLYADLYRRGRLDL
                     DRLISRTYPLEAINAAYADMLGGEIARGLIVFP"
     gene            complement(1332..1682)
                     /locus_tag="PK829_04980"
     CDS             complement(1332..1682)
                     /locus_tag="PK829_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40601.1"
                     /translation="MNNQNQKDSPFAAILRGEAPGTVIARNDDLEFAIIASIEPEAAV
                     HWIAVPYEAGQTTEMMERANRERFLQLVDYAIDETRARVADYPALGYGFTIKFHLGAY
                     ETIPHAKLHILSKE"
     gene            complement(1752..2618)
                     /locus_tag="PK829_04985"
     CDS             complement(1752..2618)
                     /locus_tag="PK829_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOU40602.1"
                     /translation="MTIAMIVHGGAGAWSRSGDRLEEARRACTAAAAAGRDILLRGGS
                     ALDAVEAAVMLLEDCPALDAGRGSYPNSEGFVEMDALIMDGRDLRMGAIAAIQRVRHP
                     VALARRVMADSGHNFLVAAGADVFADSIGFPRCDVSELLVEPAPAASDGSGPLGDTVG
                     AVAIDSDGNVAAATSTGGTANKRPGRVGDSPLVGCGAYADNWTAGVSATGYGEALMRV
                     VISKRVCDLVGAGLSAAAACEAAIRLLEERTGGDGGLIAIDARGQVGWASNTEAMPYA
                     YGIDDQPIVAGV"
     gene            2788..2859
                     /locus_tag="PK829_04990"
     tRNA            2788..2859
                     /locus_tag="PK829_04990"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2820..2822,aa:Asn,seq:gtt)
     gene            2941..4494
                     /gene="gpmI"
                     /locus_tag="PK829_04995"
     CDS             2941..4494
                     /gene="gpmI"
                     /locus_tag="PK829_04995"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOU40603.1"
                     /translation="MKEYRPVALIILDGWGIREMEHGNAVVQANKPNYDRWTRELERS
                     VVDASGEAVGLPDGQMGNSEVGHLNLGAGRIIYQDLTRINLAIERGTFAENETLLAAL
                     EGVKNRRGKLHLIGLFGEGGVHSHSKHLYALLQMAERAGVEPIIHIISDGRDTPPESA
                     LGYALTLEAYLSQHPGIVASVGGRYYTMDRDKRWARTELGYRVIALHEGYRGEEGGAP
                     RTAPSLSTALRESYAAGVTDEFVLPVAIDVGQRNVLIEPGDAVIFYNFRADRMRQIVT
                     TFTAPDFVGFERNFIPDLDVLTMTSYEANLPVKIIFPEVDITNPLAEVLSKQGCHQFH
                     AAETEKYAHVTYFFNGGRETPFEGEERHLEPSPKVATYDLQPEMSARPLVEKVIARVR
                     DHDDDFILVNFANPDMVGHTGVLSAAIKAVETVDECAGRLVAAINAKGGVAIVTADHG
                     NCERMVNEVTGEPHTYHTTQPVALFVIGDQYYDLRPRGILADVSPTILHLMGIQPPPE
                     MTGRSLISE"
     gene            4491..5048
                     /locus_tag="PK829_05000"
     CDS             4491..5048
                     /locus_tag="PK829_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40604.1"
                     /translation="MSGNAVLSANGRALRAYVDSARAALAGMTAIDYHQQPLPAPPDE
                     QLMAIVGAVAALPAADRDRFMAALTDRERALLGLFGHRAATLAVRREEPEWLRLGLLG
                     SVIANYVIPARRDVDVSLAVFHHCARKLGLSPVEVFDEAAAYASEVMAERLRAFGRRD
                     DVVLKHFGWREIRGTEGVRYKFEWR"
     gene            5045..5875
                     /locus_tag="PK829_05005"
     CDS             5045..5875
                     /locus_tag="PK829_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU40605.1"
                     /translation="MNAVEAHYDSDPAREWERLDRHRTEFAVTARVLAEHLPPPPGQI
                     LDVGSGPGRYALHLTRQGYRVTLLDLSRKSLDLALEMATREGIYLLPPVWGEATALPE
                     DYTGQFDAVLLMGPLYHLLDAAGRLAAVREAYRVLRPGGLVFAAFITRFAPLRDVAIH
                     SPQWIIDNAERFRQLLEEGLNPAYESSGFPDSYFARPEEIAPLMEAGGFQMLDLLGLE
                     GIIAGHEETVNALEGAAWEAWLALNYRLGREPALYGAADHLLYVGARPATLVAPPDLA
                     "
     regulatory      5896..6065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6243..>7186
                     /locus_tag="PK829_05010"
     CDS             6243..>7186
                     /locus_tag="PK829_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOU40606.1"
                     /translation="MMTRRVGYGALLLLALLAAVGCSMVSEDAPASGPGDAAAARAAE
                     WLVANHQNDDGGYTSFSAGANAAPSDPAGTLDALLALAAARVDTEPALAYLRANGTAL
                     EATAGEGGGQLGKLVLALDAAGADPRDFAGVDYVAALAGMMDEAGSFGAPDAFNHSLA
                     VLGLAAAGETIPAESVAWLENLQAADGSWDDGFGTAGNADATAMAIMALLAAGRSAGD
                     ASIAAAADFLAGAQADGGGWAYGAGLPTSASSTALVIQAIVALGEDPADAASRWASDG
                     QSPLDALLRFQSESGAFQTDLGSGPVDDYYTTVLAIPAA"
BASE COUNT         1163 a         2359 c         2471 g         1193 t
ORIGIN      
        1 cgacctgaat gatctggcgc tgccggctgt cgaacttctc gcggaagtag tccagctgcc
       61 agtcctcatg ccacccccag atcggctcga tgtaggcccg catggccgcg ctatgcaggg
      121 cgaacagaaa gtcgaagtcg gccagcgtgg ccgggcgcag cgtgtaggcg gtgctcatgg
      181 gaagacgatc agtccgcggg cgatctcccc gccgagcata tcggcgtagg ctgcgttgat
      241 ggcctctaga gggtaagtgc gcgagatgag ccggtcgaga tcaagccgac cgcgccggta
      301 gagatcggcg tagagcggaa agtcgcgggc cgggttggcg ctgccgtagt agcagccggt
      361 gacggtcttc tcctgccggg tgatgagcgc gccgggcaga ttggtggcgc tgcccatcgg
      421 tgagatgccg gccagaacga ccagcccgcc cggccgcgcc gccgccaaac acatctcctg
      481 cacggccggc aggccgatag cctcgaagac agtatccgcg ccgcgcccct gagtcaggtt
      541 ctggatggcg cgcacggtgt caggcccggc catgagaaag tcggtcgcgc cgaaggcccg
      601 ggccatgtcg cccttggcct tgtgcgcgtc cacggcgata atgcggctcg cgccggccag
      661 cctggccccc atgatgacgc ttaggccgac gccgccggcg ccaaagacaa ccacgctact
      721 gcctggccgg acttgggccg tgttcagcgc cgcgccgacg ccggtggtga cggcgcagcc
      781 gatgagcgcg gccacggtca ggggaatgtc cgcgcccagc gggacacaac agacctccgg
      841 cacgacggtg tactcagcga agcaggccag ggcgctgaag tggtagaccg ggcggccgtt
      901 ttgggtgagg cgtgtcgtcc cgtcgagcat ggttccagcc cagagcggcg cgatgtaggt
      961 ttcacacagg ctggggcggt tgtgcaggca gtagaagcac gcgccacaac tgggcgccca
     1021 actgagagcc acatggtcgc ccacggcgac gcgggccacg cccggcccaa cggcaacgac
     1081 cacgcccgcg ccctcatggc cgggcacgac gggcgtggcg tggcgcgtgg ctccggtcat
     1141 gaggtgccag tcgctgtggc agacgcccgc cgcagcgacg cgcaccaggg cctcgcccgc
     1201 gcccggcggg gccaaatcca gggtctccac ggcaaagggg aggcccggct cgtaaaagac
     1261 cgccgcctta accttcatcg gtcgcgtcct gcctgggcca gacctgtcag gtctggccca
     1321 ggctggctat gctactcctt cgacaggatg tgcagcttgg cgtgcgggat agtttcgtag
     1381 gcccccagat ggaacttaat ggtgaagccg tagcccaacg ccggatagtc ggccacgcgc
     1441 gcccgggtct catcaatggc gtagtcgacc agttggagaa agcgctcgcg gttggcgcgt
     1501 tccatcatct ccgtcgtttg tcccgcctca tagggcacgg cgatccagtg gacagccgct
     1561 tccggctcga tgctggcgat gatggcaaac tccaggtcgt cgttgcgagc gatgaccgtt
     1621 cccggcgctt ctccgcgcaa gatcgcggca aatggactgt ctttctggtt ctggttgttc
     1681 atgttgatct ccctgtccct tttgtcgggc gcgccgcgcg gcgcgccatt tttggttgtg
     1741 ctgccctggc tttagacgcc ggcgacgatg ggctggtcgt cgatcccgta ggcatagggc
     1801 atggcctcgg tgttagatgc ccagccaacc tgaccgcggg cgtcaatggc gatcaggccg
     1861 ccgtcgccgc cggtgcgttc ttccagcagg cggatggctg cctcgcaggc cgccgcggcg
     1921 gacagccccg cgccgaccag gtcgcagacg cgtttgctga tgaccacgcg catgagcgcc
     1981 tcgccgtagc cggtagcact gacgccggcc gtccagttgt cggcatacgc gccgcagccg
     2041 acaagggggc tgtcgccgac gcggccgggg cgcttgttgg ccgtgccgcc ggtggaagtg
     2101 gcggcggcga cattgccgtc gctgtcgatg gccacggccc cgacagtgtc gcccagtggc
     2161 ccggagccgt cactggcggc cggcgcaggc tctaccaaca actcgcttac gtcgcagcgc
     2221 gggaaaccaa tgctgtcggc gaacacgtcc gcgccggcgg cgacgaggaa gttgtggccg
     2281 ctgtcggcca ttacccggcg ggccagagcc acgggatgac gtacgcgctg gatggcagcg
     2341 atcgccccca tgcgcagatc gcgaccgtcc atgatgagcg cgtccatctc gacgaagccc
     2401 tcggagttgg ggtagctgcc gcgcccagcg tcgagcgcgg ggcaatcctc cagaagcatc
     2461 accgcggcct cgacggcgtc cagcgccgac ccgccccgca ggaggatgtc gcggccggcg
     2521 gcggccgccg ccgtgcaggc gcggcgcgct tcttccaggc gatctccaga ccttgaccac
     2581 gcgcccgcgc caccatgcac aatcatcgcg atcgtcatat gtacctctcc gtgtgcgcct
     2641 attaaacacg ctttgtgcaa tttgtcgaaa gcaggccgcg gcgattttgg cgcattttca
     2701 cttggccgcc gggggagcgc gccgggccag acgcgctcat tttggcttga caccgcagct
     2761 acagaagcta taatccgggt tcgatgttcc tcagtagctc aatggcagag cgtgcggctg
     2821 ttaaccgcaa tgttgttggt tcgagtccaa cctgaggagc tggttcaaaa acggcaggcg
     2881 gcagctctgc cgttttttat ttggcggccg ggtgagcggc tgccgaggag cgtagcgatc
     2941 atgaaagagt atagacccgt cgccctgatc atcctcgatg gctggggcat ccgcgaaatg
     3001 gagcacggca atgccgtcgt gcaggccaat aagcccaatt acgaccgttg gacgcgcgaa
     3061 ttggaacgtt cggtcgtcga tgcctcgggc gaagcggtgg gcttgcccga cgggcagatg
     3121 ggcaactccg aagttggcca cctgaatctg ggcgcgggtc gcattattta ccaggacctg
     3181 acacgcatta acctggccat tgagcggggc acgtttgccg agaacgagac cttgctggcc
     3241 gcgctggaag gcgtcaaaaa tcgccggggc aagctgcatc tcatcggcct ctttggcgag
     3301 ggtggcgtgc atagccacag caagcacctc tacgcgctct tgcagatggc cgagcgcgcc
     3361 ggcgtcgaac cgatcatcca catcatcagc gacggccgcg acacgccgcc ggaaagcgcc
     3421 ctcggctacg ccctaacact ggaagcgtat ctctcccagc atccgggtat cgtcgcctcg
     3481 gtcggcggcc gctactacac gatggaccgc gacaaacgct gggcgcggac ggaactgggc
     3541 taccgcgtga tagccctgca tgagggctac cgcggcgagg agggcggcgc gccgcgcacg
     3601 gctccatcgc tcagtaccgc cctgcgcgag tcctatgccg ccggtgtcac cgacgagttc
     3661 gtgctgcccg tggccattga cgtcggccag cgtaacgtcc tcatcgagcc gggcgacgca
     3721 gttattttct acaacttccg cgccgaccgc atgcgccaga tcgtcaccac gttcaccgcg
     3781 ccggactttg tgggcttcga gcgcaacttc atccccgacc tggacgtgct gacaatgacc
     3841 tcctatgagg ccaacttgcc agtgaagatc atcttcccgg aagtggacat taccaacccc
     3901 ctggccgagg tgctgagcaa gcagggctgc caccagttcc acgccgccga gacggagaag
     3961 tatgcccacg tgacctactt cttcaacggc gggcgcgaga cgcccttcga gggcgaggag
     4021 cgccatctgg aaccgtcgcc caaggtggcc acgtatgatc tccaaccgga gatgagcgcc
     4081 cggccgctgg tggagaaggt catcgcgcgc gtgcgcgacc acgacgacga ctttatcctg
     4141 gtcaacttcg ccaaccccga tatggtcggc cacaccggtg tcctgtcggc ggctatcaag
     4201 gcggtggaga cagtggacga gtgcgccggg cggctggtgg cggccatcaa cgccaagggt
     4261 ggtgtggcca tcgtcaccgc cgaccacggc aactgcgagc gcatggtcaa tgaggtgacg
     4321 ggcgagccgc acacctacca cacgacgcag ccggtggccc tgttcgtcat cggcgaccaa
     4381 tactacgatc tgcgcccgcg cggaatcctg gccgatgtct cgccaaccat cttgcacttg
     4441 atgggcatcc agccgccgcc ggagatgacc ggccggagcc tgatcagcga atgagcggca
     4501 acgccgtgct ctcggccaac ggccgcgccc tgcgcgccta cgtggacagc gcccgggccg
     4561 cgctggcggg catgacggcc atcgactacc accagcagcc gctgcccgcg ccgccggacg
     4621 agcaactgat ggccatcgtc ggcgcggtcg cggccctgcc cgcggccgat cgcgaccgct
     4681 tcatggccgc gctgacggac agggagcggg cgctgttggg cttgttcggc catcgcgcgg
     4741 cgacgctggc cgtgcggcgc gaggagccgg agtggctgcg actggggctg ctgggcagtg
     4801 tcatcgccaa ctacgtcatt ccggcccggc gcgacgtgga tgtgtcgctg gccgtcttcc
     4861 accactgcgc ccgcaagctg ggactgtcgc cggtggaggt ctttgacgag gccgccgcct
     4921 atgcctctga ggtgatggcc gagcgcctgc gcgccttcgg ccggcgcgat gatgtggtgc
     4981 tgaagcactt tggctggcgg gaaatccgcg gcacggaggg cgtgcgctac aagttcgaat
     5041 ggcgatgaac gcggttgagg cccattacga cagcgatccg gcgcgggaat gggagcgcct
     5101 ggatcgccac cggacggagt tcgccgtcac cgcgcgcgtc ctggcggagc atctgccgcc
     5161 gccgccgggg caaatcctcg acgttggcag cgggccgggc cgctatgcgc tgcatctgac
     5221 acggcaggga tatcgcgtta ccttgctcga cctgtcgcgc aagagcctcg acctggccct
     5281 ggagatggcc acgcgcgagg gcatctactt gctgccgccg gtgtgggggg aggcgacggc
     5341 gctgccggag gattacaccg ggcaattcga cgccgtcctg ctaatggggc cgctctatca
     5401 cctgctggac gcggccggcc ggctggcggc cgtgcgcgag gcgtatcgcg ttttgcggcc
     5461 gggcggtctg gttttcgccg cgtttatcac ccgctttgct cccctgcgcg acgtggctat
     5521 ccactcgccg cagtggatca tcgacaacgc agaacgcttc cggcaactgc tggaggaggg
     5581 cctcaacccg gcctacgagt cgagcggctt ccccgattcc tacttcgccc ggccggagga
     5641 gatcgccccg ttgatggagg ccggcggctt tcagatgctg gacttgctgg gccttgaggg
     5701 cattatcgcc gggcatgaag agacggtcaa cgctctggaa ggggcggcgt gggaggcgtg
     5761 gctggcgctg aactatcgcc tcggccgcga gccagcgctc tatggcgcgg ccgaccacct
     5821 gctttacgtt ggggcgcggc cggccacact ggttgctccg cccgatctcg cctgatagaa
     5881 taccctcaca actgcatagg caaacccgtc tcaggtcgcc ccagccccgg aaggcagggc
     5941 gtaatcgcga aactggtgcg tcttgtttgc gcaagacaag gccagtgctg tacccgcaac
     6001 tgtgattcgg ccggccaccc gccggtcgat aagccaggtc gacatcctga gatgcgtttc
     6061 tgccctcgtg gataagggtt gggaacttcg tggagattgg ttatccggct taacctcgac
     6121 tcgatgccca cgcatcgagt ttttgttccc agcgtctcgt cctgtgacca acttaagcgc
     6181 ccggcggccg cgcgcctatg aacggcggtt tgtgcccggg cggaagaggg aagaagatga
     6241 cgatgatgac acgacgagtg ggatacggcg ctctgctgct gttggctttg ctggcggccg
     6301 ttggctgttc tatggtcagt gaggacgcgc cggcgagcgg gccgggagat gcggccgccg
     6361 caagggcggc cgagtggctg gtggccaacc atcagaacga cgatggcggc tacaccagct
     6421 tcagcgcggg cgcgaacgcg gccccgtccg accccgcggg tacgctggac gcgctgctgg
     6481 cgctggccgc cgccagggtt gatacggagc cggcgctggc ctatctgcgg gccaatggca
     6541 ccgccctgga ggccacggcc ggcgagggcg gcgggcaact gggcaagctg gtgctggcgc
     6601 tggatgccgc cggagccgat ccgcgcgact tcgccggcgt cgactacgtg gccgcgctgg
     6661 cgggaatgat ggatgaggcg ggttcgttcg gcgcgcccga cgccttcaac cactcgctgg
     6721 cagtcctggg cctggccgcg gcgggggaga caattcccgc ggaatccgtg gcctggctgg
     6781 agaacttgca ggcggccgac ggttcgtggg acgatggctt tggcacagcc ggcaacgccg
     6841 acgcgacggc gatggccatc atggccctgc tggccgcggg gcgctcggcc ggagatgcct
     6901 ccatcgccgc cgcggccgac tttctggccg gggcgcaggc ggacgggggc ggctgggctt
     6961 atggcgcggg cctgcccacc agcgccagca gcaccgcgct ggtcatccag gcgattgtcg
     7021 ccctgggtga agacccggcc gatgccgcgt cgcgctgggc cagcgacggg cagtcgccgc
     7081 tggacgcgct gttgcgcttc cagagcgaaa gcggggcctt ccagaccgac ctgggcagtg
     7141 gcccggtcga tgactactac acgaccgtgc tggccattcc cgccgc
//
LOCUS       DAOSMQ010000105         7180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10439_length_7180_cov_4.646877, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000105 DAOSMQ010000000
VERSION     DAOSMQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10439_length_7180_cov_4.646877"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="PK829_05015"
     CDS             complement(<1..1757)
                     /locus_tag="PK829_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU40607.1"
                     /translation="MALILTHEKTDFDAVASMLGAKKLFPAAVALLPHHVNRNVQQFL
                     NLYWDALPFVRADDWRRRQVDSVILVDTQALSNVRGLTSQPVVHVIDHHMGQPAKPGW
                     TYEVEPLGATTTLLVERLQERGLVLAPEEATLLLLGIYEDSGGLTYDTTTVRDVRATA
                     WLLEQGAQLNVARRFLNPALSEAQQQLYDLLLHNLQWERVEERPIALAATVAPYGFND
                     EISSVAHRLRETLTPDGLFVLVRVGDGVQLVARSSVDEIDVGLVARELGGGGHSRAAA
                     AMIVGGRLPEVVQRVRAALPRAVQPTTRVAAVMSYGVLTIPTTTRIGDVAALMQKHGH
                     EGYPVVDERQGRVVGLVTRRAVDRAMSLEMGGQAVSRIMRAGNITVQPSDSIEHVQQL
                     MAAEGWGQIPVLPDAAGDGNERPIGIVTRTDLLNALFKSPPEALETDLRELLVEVFSP
                     ELWALVLVASEAAAQLKMPIYFVGGLVRDLLLGKPPTDLDIVVEGDAIALVGALRDRF
                     GGQLHSHGRFGTAKWSLSPEVYGAIAAAAESRGAAVAGHPGVGLLAPPQIDFVTARKE
                     FYSRPTVLPDVEPGSIKLDL"
     gene            1933..2772
                     /locus_tag="PK829_05020"
     CDS             1933..2772
                     /locus_tag="PK829_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40608.1"
                     /translation="MFKETGNLRDVQLLGQGGTVSRKMVIAVILGVALIAFEIFNFDT
                     TQYALSNLLGDVRFVGVLWASILAIAFCAIDFAGLVRIFTPQRGAEEPRAVWFLTGAW
                     FLGTIANAVMTWWAVSLTLLGHDFGNEVLSRATLLRYVPIFVAMLVWLTRILFIGAFS
                     VAGEDVFDQARAGESKAARRSSPQPVAQPARAARPEPQIATPPAPKPRMATPARPTPP
                     AAPTVTYEPVDDPIVGPPPTPKTNPNGALRPTSHIQKRPPMPGNGIQRAPGNMRAQGK
                     NGN"
     gene            2857..3636
                     /locus_tag="PK829_05025"
     CDS             2857..3636
                     /locus_tag="PK829_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOU40609.1"
                     /translation="MTAYQETPIYYVDSPLTQPDDDPYLPWPRSQAADDPMAAVQQRM
                     LLVLGASLAFVVVFAMWAIRMGGLNSVAVDTTSQQTAEQAAPVPMEPAATGGIAPLFT
                     REVQHWAPQIVAWAAEFGLDPNMAATVMQIESCGDPNAVSGSGAQGLFQVMPFHFSAG
                     ENMQDPDTNARRGMAYLAQGMQLTGGDTGLSFAGYNGGHGTAAKGWAAWPSETQRYYT
                     WSTGIYQDAVSGSASSETLDQWLAAGGAGLCQQAAARLGLQ"
     gene            3806..4399
                     /locus_tag="PK829_05030"
     CDS             3806..4399
                     /locus_tag="PK829_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU40610.1"
                     /translation="MSRMDLMDARRYDQGMWLLEHASLGQLRQRLLAEARGVVLEIGV
                     GTGANLPFYAPGTAVTAIELRPKRLTATTRKGYGANVAVAAADAHSLPFPAGAFDTVV
                     GTLVFCSLRDPVTVLAEVRRVLRPGGRLLLLEHVRGQTPLTRRLTDWLHPLWFALQGE
                     CHLNRETAATVAAAGFRLDHSSNHARGLVQTLIATAP"
     gene            complement(4396..5289)
                     /locus_tag="PK829_05035"
     CDS             complement(4396..5289)
                     /locus_tag="PK829_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU40611.1"
                     /translation="MIWGELAALGTAVCWSFTAIFFSYAGRRVGADVVNRSRLLFAFL
                     YLLVAHRLLQGAFLPSGVEPFRWGWLGVSSLLGLVLGDAFLFRAYVLIGPRLSMLMMS
                     TVPIFSVLFGRLLFNEAITGLETAGILLAVAGIAWVVTEGQSSDEPANQRQHRRGLLF
                     ALLGALGQVTNLVAARYALVGDFPTLSATLIRIGIALVVLWGMAAVRGQAGPTLRQWR
                     DSRALRAIMAGAIVGPFLGIWLSLVAVQNARLGIASTLMALPPVLLIPLEFAAFRQRV
                     SRRGIAGTAVALAGVALLFWR"
     gene            complement(5300..5797)
                     /locus_tag="PK829_05040"
     CDS             complement(5300..5797)
                     /locus_tag="PK829_05040"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU40612.1"
                     /translation="MADRLNRKAYQQTLPGKRISAGCLLFDEAGRLLIVKPIYKEGWE
                     IPGGTVLANESPLEGCIRETAEELGLARRPLRLLVVDYADETKDRTESLSFIFDGGVL
                     SSAEVEAIRLPPKELSEYRFLPPEQAMALLNRRLARRVRRCLGARVSGTVLYLEEEKS
                     PWASE"
     gene            complement(5782..6744)
                     /gene="mmuM"
                     /locus_tag="PK829_05045"
     CDS             complement(5782..6744)
                     /gene="mmuM"
                     /locus_tag="PK829_05045"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HOU40613.1"
                     /translation="MPTTNPIMPFIERQGALVLDGGLATELERRGANLDDALWSARLL
                     ADDPALIRQVHLDYYWAGADCCTSASYQATVPGFVTRGLSAGAARALIRRAVALTNEA
                     RDAFWAAGPHARRLRPLVAASVGPYGAYLADGSEYRGDYDLDEAGLLAFHRERWALLA
                     GCGADLLACETLPSFAEARALARLLAETPNTWAWFSFTCRDGACISDGTPIGECAAYL
                     AGRPGVVAVGVNCTPPHLIPDLIRAISAATDTPIIVYPNSGETYDPAARRWRGESAPA
                     EFGTFSREWRKLGAALIGGCCRTTPAHVRQIGDRFHKATIWPTD"
     gene            complement(6758..>7180)
                     /locus_tag="PK829_05050"
     CDS             complement(6758..>7180)
                     /locus_tag="PK829_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40614.1"
                     /translation="VYEALDPDLEKQSRPYAWYVRVPNIPAFLRHIAPALEARLAASV
                     MAGHTGTTRVNMYRQRFTLVWEAGRLREVGDNYAHDRLEDGDAAFPDLSFLQVLFGYR
                     SFEELVAHYADCYTADATTRVLLRALFPKRPSHVIPME"
BASE COUNT         1246 a         2503 c         2344 g         1087 t
ORIGIN      
        1 aggtcgagct tgatcgaccc tggctcaacg tctggcaata ccgtgggccg gctgtagaac
       61 tccttgcgcg cggtcacaaa gtcgatttgt ggcggggcca gcagccccac gccggggtgg
      121 cctgccacgg ccgcgccccg gctctcggcc gcggcggcga tggccccgta gacctccggc
      181 gacagcgacc acttggccgt gccaaagcgc ccgtggctat gcagctggcc gccgaagcgg
      241 tcgcgcagcg cccccaccag agcaatggca tccccctcga caacgatgtc cagatcggtt
      301 ggcggcttac ccagcagcag gtcgcgcacc agcccaccaa cgaagtagat gggcatcttc
      361 agctgcgccg ccgcctcgct ggccaccagc accagcgccc acaactccgg tgaaaaaacc
      421 tcgaccaaca attcgcgcag gtcggtttcc aacgcttctg gcggcgactt aaagagagcg
      481 ttcagcaggt cggtgcgcgt gacgatgccg atgggccgct cgttaccatc cccagcggcg
      541 tcgggcaaga cagggatttg cccccagccc tcggcggcca tgagctgctg gacgtgctcg
      601 atggagtcag acggctgcac agtgatgttg ccagcgcgca tgatacgact gactgcctgg
      661 ccgcccatct ccaggctcat cgcccggtcc acggcccgcc gcgtcaccag cccgacgacg
      721 cgcccctggc gttcatcgac gacgggatag ccctcgtgac catgcttctg cattagcgcg
      781 gctacgtcgc cgatgcgcgt cgtcgtcggt atggtcagaa cgccatagga cattaccgcg
      841 gcgacccgtg tggttggctg caccgcgcgg ggcaacgccg cgcgcacccg ctgcaccacc
      901 tccggcagcc gcccgccgac gatcatcgcc gccgccgccc ggctgtggcc gccgccgccc
      961 agctcgcgtg ccaccaaccc gacatcaatt tcatcgactg agctacgggc gaccagttga
     1021 acgccgtcgc caacgcgtac cagcacgaac agaccatcgg gcgtcagcgt ctcgcgcaga
     1081 cggtgggcca cagaggagat ttcgtcgttg aagccatagg gagccacggt cgcggccaga
     1141 gcgatgggcc gctcctcgac gcgctcccac tgtaaattgt gcaagagcag gtcatagagc
     1201 tgctgctgcg cttcagatag tgccgggttc agaaagcgac gggcaacgtt gagctgcgcc
     1261 ccctgctcca gcagccaggc cgtggcgcgg acgtcgcgga ccgtcgttgt gtcgtaggtc
     1321 agaccgccgc tgtcctcgta gatgcccaac agtagcagtg tcgcctcctc gggagccaac
     1381 accagcccgc gctcttgcag ccgctccacc agcagcgtcg ttgttgcccc cagcggctcg
     1441 acctcgtagg tccagcccgg cttggctggc tggcccatgt ggtggtcgat aacgtgaaca
     1501 accggctgcg acgtcaaccc tcgcacatta ctcaacgcct gcgtatcgac caggatgacg
     1561 ctatcgacct ggcgtcgtcg ccagtcatcg gcgcgcacaa agggcagcgc gtcccagtaa
     1621 agattgagga actgctgcac gttgcggttg acgtggtggg gcaagagggc cacggccgcc
     1681 gggaagagct ttttcgcgcc cagcatggag gcaacagcgt cgaaatccgt cttctcatgg
     1741 gtcaaaatga gggccatagt gggattatac atcatccctg gtcggtcgcg gattggggac
     1801 tatgcgtatt gacctggggc gaacatttgt gctatgatgc gtagcgtaga acgcctgttc
     1861 tatcaccggc aatggcggcg tgtgcagcag tagtcagacg atcataccag accgacgaga
     1921 gggaaaagcg agatgttcaa ggaaacaggc aatttgagag atgtccaact gttgggccag
     1981 ggcggaaccg tctcgcgcaa gatggtcatc gccgtcatcc tcggcgtagc cctgattgcg
     2041 ttcgagatct tcaacttcga cacgacccag tacgcgctga gcaacttgtt gggtgatgtg
     2101 cgcttcgtcg gtgtcctgtg ggcatcgatt ttggccatcg ccttctgcgc catcgacttt
     2161 gccggactgg tacgcatctt cacgccgcag cgcggcgcgg aagagccgcg cgccgtctgg
     2221 ttcctgacgg gggcctggtt cctgggcacg atcgccaacg cggtcatgac ctggtgggcc
     2281 gtgtcgctga cgctgctggg ccacgacttc ggcaacgaag tgctcagccg cgcgacgctg
     2341 ctgcgctatg tgcccatctt cgtggccatg ctagtctggc tgacgcgcat cctcttcatc
     2401 ggcgcgttct ccgtagctgg cgaagatgtc ttcgaccagg ctcgcgccgg cgagagcaag
     2461 gccgctcgcc ggtcgtcgcc gcaaccggtg gcgcaacctg cgcgcgccgc ccgcccggag
     2521 ccgcagatag ccaccccgcc cgcgcccaag ccccgcatgg ccaccccggc ccgcccaacg
     2581 ccgccagccg cgcccacagt gacctatgaa ccggttgatg atcccattgt cggcccgccg
     2641 cccacgccaa agaccaaccc caatggcgcg ctgcggccga cgtcccacat ccaaaagcgt
     2701 ccgcccatgc ccggcaacgg cattcagcgc gcccccggca acatgcgcgc ccagggcaag
     2761 aacggcaact aggggccact cttcgggggc cactcttctg ggccactcca ttcgcttgta
     2821 ttggtattag gtaacgcgag gaaacggcgg tagactatga cggcgtatca agaaacaccc
     2881 atctattacg ttgattcgcc cttgacgcag ccagacgacg atccctacct cccgtggcca
     2941 cggtcgcagg ccgccgatga tcccatggcc gccgtgcaac agcgcatgct gctggtgctg
     3001 ggcgcgtcgc tggcctttgt cgtggtcttt gccatgtggg ccatccgcat gggtgggctg
     3061 aatagcgtgg cggtggacac aaccagccaa cagaccgccg agcaagccgc gcccgtgccg
     3121 atggaaccgg ccgcgaccgg cggcattgcc ccgcttttca cgcgcgaggt gcagcactgg
     3181 gcgccgcaaa tcgtcgcctg ggcggccgag ttcggcctcg accccaacat ggccgcgacg
     3241 gtcatgcaga tcgaatcgtg cggcgacccc aacgcggtct ctggctccgg cgcgcagggc
     3301 ctcttccagg tcatgccctt ccacttcagc gccggtgaga atatgcaaga cccggacacc
     3361 aatgctcgcc gcggcatggc ctatctagcc cagggcatgc aactgaccgg cggcgacacc
     3421 ggcctgagct tcgccggcta taacggcggc cacggcacgg cggccaaggg ctgggcggcc
     3481 tggcccagcg agacgcagcg ctactacacc tggagcacgg gcatctatca ggacgccgtg
     3541 tccgggtcgg ccagcagcga gacgctcgac cagtggcttg ccgccggcgg cgcggggctg
     3601 tgccaacagg cggccgcccg cctcggtctt cagtgatctt caggcgcggc ggctcgcgcc
     3661 cgccgcgcct tcccccttgt tcagaccgcc acccaacgtg gcgcgattct cccgaatcgc
     3721 gggtcgctgt cgcgcccaag cccgcgattc gggagaatcg cgccacagat tgccccgtac
     3781 tgccccggcg ggcataatgg cgcgcatgag cagaatggac ttgatggacg cgcgccgcta
     3841 tgaccagggc atgtggctgc tggagcacgc cagcctggga cagttgcgcc agaggttgct
     3901 ggcggaggca cgcggcgtcg tgctggagat cggcgtgggc acgggggcca acctgccctt
     3961 ctacgcgccg gggacggcgg tgacggccat tgagttgcga ccaaagcgcc tgaccgcgac
     4021 gacgcgcaag gggtacggcg ccaacgtggc cgtagccgcg gccgacgccc acagcctgcc
     4081 cttccccgcc ggcgcgttcg ataccgtcgt gggcacgctc gtcttttgca gcctgcgcga
     4141 cccggtgacg gtgctggccg aggtgcggcg cgtcctgcgg ccgggcgggc ggctgctctt
     4201 gctcgaacac gtccgcggcc aaacgcccct gacacgccgc ctgacggact ggctccaccc
     4261 cttgtggttc gcgctgcaag gcgagtgcca cctcaatcga gagacggcag ccacggttgc
     4321 cgcggccggc ttccgtctcg accatagctc caaccacgcc cgcggcctgg tgcagacgct
     4381 catcgccact gcgccctagc gccagaacag cagggccacg cccgccaggg ccaccgccgt
     4441 gccggcaatc cctcgccggc taacccgctg ccggaaggca gcaaactcca gcgggatgag
     4501 caagactggc ggcaaggcca tcagcgtcga ggcgatgccc aggcgggcgt tctgcacagc
     4561 caccaacgat agccagatgc ccaggaatgg ccccacgatc gcgccggcca tgatcgcccg
     4621 cagggcgcgg ctgtcgcgcc actgccgcaa ggttggcccg gcttgcccgc gcacggcggc
     4681 catgccccac agcacaacga gggcaatgcc aatgcgaatg agcgtcgccg agagagtcgg
     4741 gaagtcaccg accagcgcgt agcgggccgc gaccaggttg gtcacctgcc ccagcgcgcc
     4801 cagcagggcg aacagcaggc cgcgccgatg ctgccgttgg ttggccggct cgtcgctact
     4861 ttgcccctcg gtcacgaccc aggcgatgcc ggctacggcc agcaggatgc cggctgtctc
     4921 cagcccggtg atggcttcgt tgaagagcag ccggccgaag aggacgctga agatgggcac
     4981 ggtggacatc atcagcatcg acagccgcgg cccgatgagc acgtaggcgc gaaagaggaa
     5041 agcgtcgccc agcaccaggc ccagcaggct ggacacaccc agccatcccc agcgaaacgg
     5101 ctcgacaccg gagggcaaga acgcgccttg cagcaggcgg tgggccacga gcaggtagag
     5161 aaaggcgaac agcaaccggc tgcgattcac cacatccgca ccgacgcgcc ggccggcgta
     5221 gctgaagaag atggctgtaa acgaccagca cacggccgtg cccagcgcgg ccaactcgcc
     5281 ccagatcatc gcgccggcct cattcgctcg cccatggcga cttctcctct tcaagatata
     5341 gcaccgtgcc gctgacccgc gcccccaggc agcgccgcac ccgtcgcgcc agacggcgat
     5401 tgagtagcgc cattgcctgc tccggcggca ggaagcgata ctcgctcagc tccttcggcg
     5461 gcaggcgaat agcctccacc tcggccgacg acagtacgcc gccgtcaaag atgaagctca
     5521 ggctctcggt gcgatccttg gtctcatcgg catagtcgac gaccaacaac cgcagcggcc
     5581 gccgcgccaa ccccaactcc tcggccgtct cgcgtatgca gccctccagc ggcgactcat
     5641 tggccagcac cgtcccgccg ggaatctccc agccttcctt gtagatcggc ttaacgatga
     5701 gcagccggcc ggcttcgtcg aacagcaggc agccggcgga gatgcgcttg cccggcaggg
     5761 tctgttggta cgccttgcgg ttcagtcggt cggccatatg gtcgccttgt ggaagcggtc
     5821 gccgatctgg cgaacgtggg ccggcgtggt gcgacagcaa ccgccgatga gggccgcgcc
     5881 cagcttgcgc cactcgcggc tgaacgtgcc gaactccgcc ggcgcagact cgccccgcca
     5941 gcggcgcgcc gccgggtcgt aggtttcgcc ggagttgggg tagacgatga tcggcgtgtc
     6001 ggtggcggcg ctgatggccc ggatgaggtc ggggataaga tgcggcggcg tgcaattgac
     6061 gccgacggcg accacacccg gccgccccgc cagataggcg gcgcactcgc caatgggcgt
     6121 gccgtcgctg atacacgcgc cgtcacggca agtgaagctg aaccaggccc acgtgtttgg
     6181 cgtctcggcc agtaaccggg ccagcgcccg cgcctcggcg aaggaaggca acgtctcgca
     6241 agccagcagg tcggccccgc acccggccag cagcgcccaa cgctcgcggt ggaaggccag
     6301 tagcccggct tcgtccaggt cgtaatcgcc gcggtactcg gagccgtcgg ccaggtacgc
     6361 gccataaggg ccgacgctgg cggcaacgag cggccgcagc cgccgcgcgt gcggccccgc
     6421 ggcccagaag gcgtcgcgcg cctcgttggt caacgcgacg gcgcgccgga tgagcgcccg
     6481 cgccgcgccg gcgctcagac cgcgagtcac gaagccgggc accgtagcct ggtagctggc
     6541 gctggtgcag cagtcggccc cggcccaata gtagtcgaga tgcacctggc ggataagcgc
     6601 cgggtcgtcg gccagcagcc gcgccgacca gagggcgtcg tcgaggttcg cgccgcgccg
     6661 ctccagttcg gtggccagcc cgccatcgag caccagcgcg ccctggcgtt caatgaaggg
     6721 cataatcggg ttggtcgtgg gcataggcac gaccggccta ctccatcggg ataacgtgcg
     6781 acggccgctt gggaaacagc gcccgcaaaa gcacgcgcgt cgtggcgtcg gcggtgtagc
     6841 agtcggcgta gtgggcgacc aactcctcga agctgcgata gccgaacagt acttgcagga
     6901 acgacagatc ggggaaagcg gcgtccccgt cttccagccg gtcgtgggcg tagttgtcgc
     6961 ccacctcgcg caggcggccg gcctcccaaa ccagcgtgaa gcgttggcgg tacatgttga
     7021 cgcgcgtcgt gcccgtgtgg ccggccatga cgctggcggc caggcgcgcc tccagcgccg
     7081 gcgcgatgtg gcgcaggaag gcggggatgt tgggcacgcg cacgtaccag gcatagggcc
     7141 ggctctgctt ctccaggtca gggtcaagcg cctcgtagac
//
LOCUS       DAOSMQ010000106         7170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10464_length_7170_cov_4.723682, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000106 DAOSMQ010000000
VERSION     DAOSMQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10464_length_7170_cov_4.723682"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PK829_05055"
     CDS             complement(<1..356)
                     /locus_tag="PK829_05055"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOU40615.1"
                     /translation="MSLSWNPPSGSPVTGGTGGVVSTGLWFGEYYNNKNFIGSPTFTR
                     NDAAIDFNWGAGSPGAGIPVDGFAVRWTRNAVVTAGNYRFDVFSDDGIRVWVNNLLII
                     DQWRDQPAGNFSATVSL"
     gene            complement(353..1198)
                     /locus_tag="PK829_05060"
     CDS             complement(353..1198)
                     /locus_tag="PK829_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOU40616.1"
                     /translation="MSKNLNRTLIPLLALLGGLLLLALGPGAPRAAAADSAWRARYWN
                     NRTLSGDPVVQRDEAEINNDWGGGKPHERINDDNFSVRWTRAVDFSAGNYRFTATMDD
                     GMRVWIDDQLLIDAWTDSQVRTLQADRVLTGGTHNIRIDYFEAGGVAVAKFNWQQVGA
                     APAGVFTAWKGEYFNNMNLSGQPSFLRDDREINFNWSVGSPAAGIAADQFSVRWTRQL
                     NFPAGTYRFDVFADDGVRLWVNNQLVIDQWQNQSEGRFSANVTLPAGRSRCGWSTSRT
                     RAARQ"
     gene            complement(1349..3397)
                     /locus_tag="PK829_05065"
     CDS             complement(1349..3397)
                     /locus_tag="PK829_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOU40617.1"
                     /translation="MAAIGIDTGGTFTDFIWLDEAGQLRLHKEPSTPADPSQAVLAGL
                     AASGATTASVVHGSTVATNALLQRRGARTALIATEGFADVLAIGRQNRPDLYALVPQK
                     PPPLVPAAWRLEARERISAGGEVLVPLDEAALAAALTRLDAGDVESVAVCLLFSFLRP
                     EHERRIGALLAERFGPALHVSLSSDILPEYREFERTATTVINAYVAPLMGRYLTRLAG
                     RLAPRRLAVMQSNGGVISAALAGAQAARTVLSGPAGGVVGARFVAEAGGAGYADVITF
                     DMGGTSTDVALCAGRAPTTTEGEIAGLPLRLPLIDIHTVGAGGGSLAYLDAGGALLVG
                     PQSAGAEPGPAAYHADYAAWRAALDGHFAAGQATVTDANLVLGRLDARRFLGGQMALH
                     EGSATRAVGALAAALGVPVEQAALAVVRVANATMERAIRRISVERGHDPRRFTLVAFG
                     GGGPLHACELAAELRIPRVLVPLTPGVLSALGMLVAAPARDTSRTVMARLDGLTAGDI
                     AAVFAPLMAQARREMADEGHDPAALALRGALDLRYAGQSHELTVAAPLDADGAAMAAA
                     FHAAHTARYGYARPEAAVELVTARLTATAAHTPPVQPSFAAGPPAAGEALVGQRAVWF
                     AGGPAESALYDRERLRAGHRLAGPAVVYQYDTTTLIPPGWGAWVDEWLNLIVEPEAGL
                     "
     gene            complement(3412..4356)
                     /locus_tag="PK829_05070"
     CDS             complement(3412..4356)
                     /locus_tag="PK829_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40618.1"
                     /translation="MDYSGTLRRGWDITWQNKWLFLLTLLPFAVSLISLPLSLLNGAV
                     DPTAAPAEVVAFAGRSLLLACVSLILSLFAAILTLVTHAARAAGVARAARGEATGFGR
                     SWGEGWRALPRLLGLALLLYVPLIVLFIVAFLVVLVPVTMASLSGDPGQSGIMAGMGT
                     VGVILFCCLYIAFVLVLILVGCIFTFAGRGIVLRNLGVMASLRHGWQVVRENFGQNIL
                     LALPFGLIYLVLGLVFGALFVLLAFPDGFESAVMTGNPMMLNGRMLLAYIIYLLPAVV
                     LTTWQGATVTLGYLHWTGKDVLGATTTPPDMPPAAPLM"
     gene            complement(4462..5436)
                     /locus_tag="PK829_05075"
     CDS             complement(4462..5436)
                     /locus_tag="PK829_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40619.1"
                     /translation="MDYMGTLQRAWRLTWANKWLWLLGFLAALGGGVTSTSNYRFDSS
                     NTTPSQITPQMGMALAGGALVLMCVFFIIGILLWLVTLAAKGGLITSVADLDRGQTSS
                     FGRAFRQGWRKVLPLVGMTLVLNAVIIVLLIILLVGFAGTALATLAAGGNMDSNALTG
                     ILGTAGVVFLCLLCLLIPLAIVINLIYPFAYRGMMLRDLGVMDSIRHGWRVLRENLSH
                     ILLLGLLFFVIGLVVGLISGAIVGLLGLSTGLISMILNGSQPTTTQMVAAGLGFLVTA
                     IIFAIISAIITAWRSATFTLAYEQWMGQDMLKDTAAPMPPAPPVAPMS"
     gene            complement(5504..7060)
                     /gene="guaA"
                     /locus_tag="PK829_05080"
     CDS             complement(5504..7060)
                     /gene="guaA"
                     /locus_tag="PK829_05080"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU40620.1"
                     /translation="MHDTIIILDYGSQYAQLIARRVREANVYCELLPWQTPAETVLAL
                     RPKGFILSGGPNSVYDPGAPTLPGYVLDAGVPVLGICYGMQLLAHRLGGRVAHSDRRE
                     YGPAALVVDEAGEPLFAGWGAGDGRGDLVWMSHGDKVEALPPGYRPIAHSDNSPFAAA
                     ANAQRGHYAVQFHPEVAHTPRGRVLLSNFVHRICGCRPDWTPANVIDAQTASIQQQVG
                     AGRVVLGLSGGIDSAVAAALIHRAIGAQLTCIFVDTGLLRLGEATQVVQAFQQEQGIR
                     LVAVNAVEEYLEALTGVTDPEQKRRVIGEKFVRIFEREARRLGQIDFLAQGTIYPDVI
                     ESAGKGRGAAHVIKTHHNVGGLPDDMQFQLVEPLRMLFKDEVRRLGTALGLPDSLVWR
                     QPFPGPGLAVRCLGDVTWERLERLRAADAVFLEELTAADLLREGTQQAFAVLLPVRSV
                     GVMGDGRTYQEVVALRAVTTEDFMTADWARLPYDLLARVSRRIVNEVPGVNRVVYDIT
                     SKPPGTIEWE"
BASE COUNT         1256 a         2492 c         2376 g         1046 t
ORIGIN      
        1 aggctgacgg tcgcgctgaa gttacccgcc ggctggtcgc gccactggtc gatgatcagc
       61 aggttgttga cccagacgcg aatgccgtca tcggagaaca cgtcaaagcg gtagttaccg
      121 gcggtcacga ccgcgttgcg cgtccagcgc acggcgaagc catcgacggg gatgcccgcc
      181 cccggcgatc ccgcgcccca gttgaaatcg atggccgcgt cgttgcgcgt gaacgtcggt
      241 gagccgatga agttcttgtt gttgtagtac tcgccaaacc aaaggccggt ggagacgacg
      301 ccgcctgtgc cgcccgtcac cgggctgccg ctgggcgggt tccacgacag actcactgcc
      361 gcgcggccct ggttctcgaa gtactccagc cgcagcggga tcgtccagcc ggcagggtga
      421 cgttggcgct gaagcgcccc tcggactggt tctgccactg gtcgatgacg agttggttgt
      481 tgacccacag ccgcacgccg tcgtcggcga aaacatcaaa gcgatacgtg ccggcgggga
      541 agttgagctg gcgcgtccag cgcaccgaga actggtcggc ggcaatgccc gccgccggcg
      601 agccgacgct ccagttgaag ttgatctcgc ggtcgtcgcg cagaaaggac ggctggccgc
      661 tgagattcat gttgttgaag tactcgccct tccaggcggt gaagacgccg gccggcgcgg
      721 cgccgacctg ctgccagttg aacttggcca cggccacgcc gcccgcctcg aaatagtcga
      781 tgcggatgtt atgcgtgccg ccggtcagga cgcggtcggc ctgcaacgtg cgcacctggc
      841 tgtccgtcca ggcgtcgatg agcagctggt cgtcgatcca gacgcgcatg ccgtcgtcca
      901 tcgtcgccgt gaagcgataa ttgccggccg agaaatcgac cgcgcgggtc cagcgcaccg
      961 agaagttgtc gtcgttgata cgttcatggg gcttgccgcc gccccaatcg ttgttgattt
     1021 ccgcttcgtc ccgttggacg acgggatcgc cggagagtgt gcggttgttc cagtagcgcg
     1081 cccgccaggc cgaatcggcc gcggccgcgc gtggcgctcc tggccccaat gccaggagca
     1141 gcaaaccccc cagcagtgcc agcaggggga tcaacgtgcg gttcaggttc ttggacatag
     1201 gacaggctta ctccttacgt gaatcgtctc tggccgcaga ggtgaggaaa ggcgacggtg
     1261 gtgcggcgaa atactctgag tctttctatt ataaggttgg tagcgacgcc aaagaggcgg
     1321 cgctcatcac ccatttaaag taaagacgct atagccccgc ttctggttcc acaatcaggt
     1381 tcaaccactc atcgacccag gccccccagc caggtgggat gagcgtcgtc gtgtcatact
     1441 ggtagaccac ggccggcccg gccagacggt ggccggcccg cagccgctcg cggtcgtaga
     1501 gggcgctctc ggccggcccg ccggcaaacc agaccgcccg ctggccgacc agggcttcgc
     1561 cggccgcggg cggcccggcg gcgaacgagg gctggacggg cggcgtgtgg gctgccgtgg
     1621 ccgtcagccg cgccgtgacc agttcgacag ccgcctccgg ccgggcgtag ccatagcggg
     1681 ccgtgtgggc ggcgtggaaa gcggcggcca ttgccgcccc gtcggcgtcg agcggcgcgg
     1741 ccacggtcaa ctcgtgtgac tgcccggcgt agcgcaaatc cagcgccccc cgcagcgcca
     1801 gcgccgccgg gtcgtggccc tcgtcggcca tctctcgccg cgcctgcgcc atcagcggcg
     1861 cgaagacggc ggcgatgtcg ccggcggtca gcccgtcgag ccgggccatg accgtgcgcg
     1921 aggtgtcccg cgccggcgcg gccaccagca tgcccagcgc cgacagcacg cccggcgtca
     1981 ggggcacgag gacgcgcgga atgcgcagtt cggcagccaa ctcgcaggca tgcagcggcc
     2041 cgccgccgcc aaaggccacc agcgtgaagc ggcgcgggtc atggccgcgc tcgacggaga
     2101 tgcgccggat ggcccgctcc atcgtcgcgt tggccacgcg caccacggcc agcgccgcct
     2161 gctccacggg cacgcccagc gccgccgcca gcgccccaac ggcccgcgtc gctgatcctt
     2221 cgtgcagcgc catctgcccg ccgaggaagc gtcgcgcgtc cagccgcccc aggacgaggt
     2281 tggcgtcggt cacggtggcc tggccggcgg cgaagtggcc gtccagcgcc gcccgccagg
     2341 cggcgtagtc ggcgtggtag gcggccggcc ccggctccgc cccggcgctc tgcgggccga
     2401 ccagcagcgc cccgcccgcg tccagatagg ccaggctgcc cccgcccgcg ccgacggtgt
     2461 gaatgtcgat cagcggcagg cgcagcggta gcccggcgat ctcgccctcg gtcgtcgttg
     2521 gcgcgcggcc ggcacagagg gccacgtcgg tgctcgtgcc gcccatgtcg aaggtgatga
     2581 cgtcggcgta gcccgcgccg cccgcctcgg ccacaaagcg cgccccgacc acgccgccgg
     2641 ctggcccgga aagaaccgtc cgcgccgcct gcgccccggc cagcgccgcg ctgatgacgc
     2701 cgccgttgct ctgcatgacc gccagccggc gcggggccag ccgcccggcc agccgcgtca
     2761 gatagcggcc catcagcggg gcgacgtagg cgttgatgac cgtcgttgcc gtgcgttcga
     2821 attcgcggta ctccggcaag atgtcggacg acagcgagac gtgcagcgcc gggccgaagc
     2881 gctcggccag cagcgcgcca atgcggcgct cgtgttccgg gcgcaggaag gagaagagta
     2941 aacagacagc caccgactcg acatcgcccg cgtccagccg cgtcagcgcc gccgccaggg
     3001 ccgcctcgtc cagcggcaca agcacctcgc cgccggcgct gatccgctcg cgcgcttcca
     3061 gccgccacgc cgccggcacc agcggcggcg gcttctgcgg caccagggcg tagaggtcgg
     3121 ggcggttctg gcggccgatg gccagcacgt cggcaaagcc ctccgtggcg atcagcgccg
     3181 tgcgcgcgcc gcggcgctgc aagagggcgt tggtggccac ggtgctgccg tggacgacgc
     3241 tggctgtggt cgcgccggac gcggccagcc cggccagcac ggcttgcgac gggtcggccg
     3301 gcgtgctcgg ctccttgtgc agccgcaatt gcccggcctc gtccagccag atgaagtcgg
     3361 tgaaggtgcc gccggtgtcg atgccaatcg cggccatagg cataggccca gctacatgag
     3421 cggcgcggcc ggcggcatgt cgggcggcgt ggtcgtcgct cccagcacgt ccttgcccgt
     3481 ccagtgcagg tagcccagcg tcaccgtggc cccctgccac gtcgtcaaca cgacggcggg
     3541 tagcaggtag atgatgtagg ccagcaacat tcgcccattg agcatcattg gattgcccgt
     3601 catgacggcg ctttcgaagc catctggaaa agccagcaac acgaagagcg cgccgaagac
     3661 aagccctaaa accaggtaga ttagtccgaa cggcagcgcc aggagaatgt tctgcccgaa
     3721 gttctcgcgc acaacctgcc agccgtggcg cagcgacgcc atcacgccca ggttgcgcag
     3781 cacgatgccg cggccggcaa acgtgaagat acaaccaacg agaatgagca ccagtacgaa
     3841 agcgatgtag aggcagcaga acaggatcac gcccacagtg cccattcccg ccatgatccc
     3901 actctggccc gggtcgcccg acagcgaggc cattgtcacc ggtactagca ccacgaggaa
     3961 agcgacaatg aatagcacga tcagtgggac gtagagcagc agtgccaggc ccaacagccg
     4021 cggcagcgcc cgccagccct cgccccacga gcggccgaag cccgtggcct cgccacgcgc
     4081 cgcccgcgcc acgccggccg cgcgggcggc gtgggtgacc agcgtcagga tggcggcgaa
     4141 caggctgaga atcaggctca cgcaggccag cagcagcgac cggccggcaa atgccactac
     4201 ctctgccggc gcggccgtcg ggtcaaccgc gccattgagc agcgacagag gcaggctgat
     4261 caggctgacc gcgaatggca acagtgtcag cagaaacagc catttattct gccaggtgat
     4321 gtcccaaccg cgacgcagcg taccggaata gtccacaagt ccctcccttg gcaacccgga
     4381 ggcggtcgcc caatgccaag taaatcaggc agagcgatct ccgcgaccgc tctgcctgac
     4441 gacacagtga agacctgaag cctagctcat tggcgccact ggcggcgcgg gcggcatcgg
     4501 tgcggccgtg tccttgagca tatcctggcc catccactgc tcataggcca gcgtgaacgt
     4561 cgccgagcgc caggcggtga tgatggccga gatgatggcg aagatgatcg ccgtgaccag
     4621 gaaacccaga cccgcggcca ccatctgcgt ggtcgttggc tggctgccgt tcaggatcat
     4681 actgatcaag ccggtactca gccccagcag gcccacgata gccccggaga tgagaccaac
     4741 aaccagcccg atgacgaaga acagcaggcc cagcaaaagg atgtggctca ggttctcgcg
     4801 caggacgcgc cagccgtggc ggatggagtc catcacgccc aggtcgcgca gcatcatgcc
     4861 ccggtaggca aacgggtaga tcaggttaat cacgattgcc agcggaatca gcaggcagag
     4921 caggcacagg aagaccacac ctgccgtgcc caggataccg gtcaacgcgt tgctgtccat
     4981 gttccctccg gcagcgaggg tggccagcgc cgtccccgcg aagccgacca acaggatgat
     5041 cagcaggacg atgatcacgg cattcagcac cagcgtcata cccaccagcg gcagaacctt
     5101 gcgccagcct tgccggaagg cgcggccaaa gctcgatgtc tggccgcggt caaggtcggc
     5161 gaccgaggta atcaagccac ctttggctgc cagggtgacc agccacagga ggatgccgat
     5221 aatgaagaag acgcacatca acaccagcgc gccgccagcc agggccatgc ccatctgcgg
     5281 cgtgatctgg ctgggcgtcg tgttgctcga atcgaaacga tagttggagg tgctagtgac
     5341 gccgcctccc agagctgcca gaaaaccaag cagccacagc cacttgttgg cccatgtcag
     5401 ccgccaagct cgttgcagag tacccatgta atccataatg tcactctcct taaactcata
     5461 ctacactaat cccagtacgc gggaaagagt agttagttgc aatttattcc cactcaatgg
     5521 ttcctggcgg tttggaggtg atgtcataga ccacgcgatt gacgccgggc acttcgttga
     5581 cgatgcgccg gctgacccgc gccagcaggt catagggcag gcgcgcccag tcggcggtca
     5641 tgaagtcctc ggttgtcacg gcgcgcaggg cgacaacttc ctggtacgtc cggccgtcgc
     5701 ccatcacgcc gacgctgcgc acgggcagca gcacggcaaa cgcctgttgc gtgccctcgc
     5761 gcagcaggtc ggccgcggtc aattcctcca ggaagacggc atcggccgcc cgcagccgtt
     5821 ccagccgctc ccaggtcacg tcgcccagac agcgcacggc cagccccggc ccggggaacg
     5881 gctgccgcca gacgaggctg tctggcaacc ccagcgccgt gcccagccgc cgcacctcgt
     5941 ccttaaacag catccgcaat ggctcgacca gctggaactg catgtcgtct ggcagcccgc
     6001 cgacgttgtg gtgcgtcttg atgacgtggg ccgcgccgcg ccccttgccg gcgctctcga
     6061 tcacgtccgg gtaaatcgtc ccctgggcca gaaagtcgat ctggcccagc cgccgggcct
     6121 cgcgctcgaa gatgcgcacg aacttctcgc cgatgacgcg ccgcttctgc tccgggtcgg
     6181 tgacgccggt cagcgcctcc agatactcct ccacggcatt gaccgccacc aggcggatgc
     6241 cctgctcttg ctggaaggcc tgcaccacct gggtcgcctc gcctagccgc aacaggccgg
     6301 tatcgacaaa gatgcacgtt agctgcgcgc caatggcccg atggatgagc gccgcggcca
     6361 cggccgaatc gatcccgccg ctcagcccca gcacgacgcg accggcgccc acctgctgct
     6421 ggatggacgc cgtttgcgcg tcaatcacgt tggctggcgt ccaatcgggc cggcagccgc
     6481 aaatgcgatg gacgaagttg ctcagcagca cccggccgcg cggcgtgtgg gcgacttcgg
     6541 ggtggaactg cacggcgtag tggccgcgct gcgcgttggc cgcggcggcg aagggggagt
     6601 tgtcgctgtg ggcgatgggc cggtaaccgg gcggcagcgc ttcgaccttg tcgccgtggc
     6661 tcatccagac caggtcgccc cggccgtcgc ccgcgcccca gccggcgaag agcggctcgc
     6721 cggcctcgtc gaccaccagc gccgccggcc catactcgcg ccggtcgctg tgggcgacgc
     6781 gcccgcccag ccggtgggcc agcagctgca tgccgtagca gatgcccaac accggcactc
     6841 ccgcatcgag cacgtagccg ggcagcgttg gcgcgccggg gtcatagacg ctgttcggcc
     6901 cgccgctgag gatgaagccc ttcggccgca gcgccagcac cgtctccgcc ggcgtctgcc
     6961 agggcaacaa ctcgcaatag acgttcgcct cgcgcacccg ccgggcgatc agttgggcgt
     7021 actgggaacc gtagtcgagg atgataatcg tgtcgtgcat cgggggagtg gtcagtggcc
     7081 agtagatcgc taaatcagta gtctgtcgtc tgtcggcggt ggtcggcggt cgtccgttgc
     7141 catccaaccg gctacaatcc acccaacgac
//
LOCUS       DAOSMQ010000107         7156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10491_length_7156_cov_4.288973, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000107 DAOSMQ010000000
VERSION     DAOSMQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10491_length_7156_cov_4.288973"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK829_05085"
     CDS             <1..315
                     /locus_tag="PK829_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007257813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOU40621.1"
                     /translation="DAVEEAVSPAQEKVAEVVAETRDEAEEVVAEVKTRARKVEAAVV
                     PDDLTQIKGIGPAFAQRLTAAGYTTYADIANATPDALREATHAPAIADPDEWIAGARA
                     RM"
     gene            complement(812..1201)
                     /locus_tag="PK829_05090"
     CDS             complement(812..1201)
                     /locus_tag="PK829_05090"
                     /inference="COORDINATES: protein motif:HMM:NF039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HOU40622.1"
                     /translation="MSIQIIVDLPDHIYRRLESLARQSQRDVNAVITEVVSRSVQPFP
                     VDANRAAMLREVEAFRVLQPTLWHDLPNEHVAILDGKLVDHDADPVALLQRIRRDYPG
                     RTVLRRKVGPASDRVLRFRSPRLSPRP"
     gene            complement(1354..1929)
                     /locus_tag="PK829_05095"
     CDS             complement(1354..1929)
                     /locus_tag="PK829_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_272502268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1789..1791),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HOU40623.1"
                     /translation="MTKEKKLAPGDTAPDGRCLDKSGQEQQLSTFWQEGPVLLTFLRH
                     FGUIHCREWLAQLELHREEIEAAGLRIVALGLGEPKHARQFGDKLAPNVDCLTNEEPV
                     LHAAFGIERGNLLRMLAPDAIRAGARAASRGHSQGQATGDSQRLPGTFIIDRDGIVRY
                     AYYGKFAGDQPELTELVAWWRENGEMQGRAE"
     gene            complement(2015..3502)
                     /locus_tag="PK829_05100"
     CDS             complement(2015..3502)
                     /locus_tag="PK829_05100"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU40624.1"
                     /translation="MILEVILILVALHLLLRPLLLAWQFSRPLRRPLSGHTPADWGAD
                     YEDVEFAGGDGAPLRGWYIPSRNGTAVVLLHGHGGNRLSVAFHAATLARAGYGVLLFD
                     LRAHGQSGGRPFSRGERGVDDVLAAVAWLSRRRDVQARVGVLGISVGGMLAIQAAAHN
                     VFIRAVMADGPLLGTIDDLPPPRGWFERLWRFPRERGYQRAIDWFAPGPRPPANMQAL
                     ARLGGRPVLLISTGRGLEQRLTRHFFAAAAEPKTLYEIPDAAHAMGWVVAPKAYERQM
                     LDFFGHALSLEDTLAEGTRIDVAPVAALADAPSPPSPRAVTERTVPLPVAMMLAFGTI
                     PVAAMALFIPFQLRWGLTPPQLPERWPVTALLAVFALLLGGLLLREAVLLAGYRWIGR
                     VPRGAARLAAGHAALGPRVRCDAPVPARAYRLILLLPTLLLGVLPGVAAIVAGSWLLV
                     LWGLWMIVACSGDLVALWAMRGLPPATPVRAHPSRPGCEVLSLDS"
     gene            3593..4945
                     /locus_tag="PK829_05105"
     CDS             3593..4945
                     /locus_tag="PK829_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40625.1"
                     /translation="MRIRAARLLLPLAFLVLLVAFVIIWRARSQGVFGPAVALCPGPD
                     RYGYTCEGGAAYATIDAADATGLLADDAVITLALPFPFTFYGATYTDVNVSTNGNLQF
                     TTDNPLPRPDCLAPAAGMGDLIAPYWADLDLTAHGALETAVVGEAPARVFVVEWDDVP
                     LYGTDSSDRVTFEVQLFETSNDIVFLYEDPTTAGGGNGGAAVAGIQSERQGLSLSYSC
                     RQPVLPAPGGLRLAHPAEPNRDAGQPEDAPAAVGPAMSGPAAAAPPAKGPLAELLAAY
                     ETRGPAALAALQRQWAGGRPARAFVWRAADLTGDGTAELVAAWGGGPGAPELTEVAVL
                     AVGAGQPPALLFDRRLSTRAAGYAAVNVAEVADLTGDGGDDVVLRDQKTGTTWVLTWA
                     LPAAAGEPALLDVPARCGGDLIIRDTDGDGRLELIRDGCDRPGRVSVAWDGAAFVIAP
                     "
     gene            5077..5763
                     /locus_tag="PK829_05110"
     CDS             5077..5763
                     /locus_tag="PK829_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40626.1"
                     /translation="MFELNGVYRNRIGEYTVVALNGPRMVVRYADGTEAELNVNIQAR
                     IWENIVAEQEARAASSRHLRQSNKDTTHYIKAVSLPPGEELAFPGWQERVVMAPTPEL
                     AQRLKSGDRFIYYAVEAQTFFAVVTITGEVFQADPKQHTFTVEADKAFFFPTDVDAAS
                     ANLEHGVSADSIELESYPDFGKLHIEPESFFRISEDDFELLAEALTEITEDEEEETEE
                     FEEYEEDEVE"
     gene            5756..>7156
                     /gene="metG"
                     /locus_tag="PK829_05115"
     CDS             5756..>7156
                     /gene="metG"
                     /locus_tag="PK829_05115"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOU40627.1"
                     /translation="MNKAQSENILVCIAWPYANSDIHQGNVTGSHLPGDIYARFQRLR
                     GHHVVMVSGSDSHGTPVTVKAEELGRSNLETYELYHARFLELFRRLGISYDLFTSTHT
                     ENHFRVSQDMFLQLLKNEYLYRKVTPQMYSPEAGQFLPDRYVEGTCPNCGYTGARGDQ
                     CDNCNTLFESAAELLNPRSKRDNTSLVLRDTEHFFIDLPAIAAGALSESIHDDKQHWR
                     PQVVNFARNFIDQGLIGRAVTRDMDWGIPVPVDEGYDEKVLYVWFEAVIGYLSATIEW
                     AHNQDDNDAWRDWWQNPDARTLYFIGKDNIPFHTVIWPAQLLGARGLYSDNPQATLNL
                     PYDVPANEFMNMEGRKISGSRNWGVWMLDALDRYDPDPLRYYLTTNMPEARDSDWTWS
                     GFIERNNNELVANWGNLVNRVLNMIRRYFGGVVPEPGALTAADEALLAAIDDGFDSVA
                     TFYDGCKFRAAVQEALR"
BASE COUNT         1277 a         2411 c         2342 g         1126 t
ORIGIN      
        1 gacgcggtcg aggaggcggt gtcgccggcc caggagaaag tcgccgaggt cgtcgccgag
       61 accagggacg aggccgaaga agtggtcgcc gaggtcaaga cgcgggccag gaaggtcgag
      121 gcggcggttg tgcccgacga cctgacccag atcaagggta tcgggccggc ctttgcccaa
      181 cgcctgaccg ctgccggcta caccacttac gccgacatcg ccaacgccac gcccgacgcc
      241 ctgcgcgagg ccacccatgc cccggccatt gctgacccgg acgagtggat cgccggcgcg
      301 cgggcacgga tgtaaatgaa agtgccgcgc ccgaactggc cgcggcacag ctgcccataa
      361 ccgacagcta cttggtcttg ctatttgctc gccaccgcgc tagcgcgatc tagcagatcg
      421 cgctatgcgt ctgattctac ttgtgtcgta aacgccgggc cgttgagggt taccacaaga
      481 ttgttgagta catcccgacc gataacgctt tccgtcccag ttggtatggc tacggcctga
      541 attccgtgga cagttagtaa tcccaaccga atggcaaccg tgtaccgatc cacttgctcg
      601 ctttcgccca atacgccccg catctgcacc gtatcttcgt agagtgcgcc gaccgcttga
      661 aggacatcca ccggcagcat cgtcccgtct gcgcctgagt cgactaacgc aatgagtggt
      721 tgtggtgggt gcccggcgta gccatccacg gttactgtta ccacgggcgt ggccgggtaa
      781 taggactcat catacgggaa gtctattcgg ttcacggtcg cggcgaaaga cgaggagaac
      841 ggaagcgcaa aacacggtcc gaggccggcc ccactttgcg gcggagtacc gtccggccag
      901 ggtaatcccg tcgtatgcgc tggagaagcg cgacagggtc ggcgtcgtga tcgaccagct
      961 tgccgtccag aatcgcgacg tgctcattgg ggaggtcatg ccacaaagtc ggctgcaaca
     1021 cccgaaacgc ctccacctcg cgcagcatcg cggcccgatt ggcgtccacc ggaaacggct
     1081 ggacggaacg gctaaccacc tcggtaatga ccgcattcac atcccgctgc gattgacgcg
     1141 ccagcgattc aagacggcga tagatgtggt cgggcagatc gacaatgatt tggatgctca
     1201 tttgtcggtt atcttaatga cagcagaggc cgcgaaagca gcctcgtacc cggcttccga
     1261 agaaccggag gatatcatcg gaatagtact tgaggaatgt aggtagcgca ataggccttt
     1321 agagggacac ggatgcctgg ggcacacttt cgactactct gcccttccct gcatttcccc
     1381 attctcccgc caccacgcca ccaactccgt caactccggc tggtcgccgg cgaacttgcc
     1441 atagtaagcg tagcggacga tgccgtcgcg gtcgatgatg aacgtgcccg gcaagcgctg
     1501 gctgtcgccc gtcgcctggc cctggctgtg cccgcgcgag gcagcgcgcg cgccggcgcg
     1561 gatggcatca ggggccaaca tgcgcagcag gttgccccgc tcgatgccga aggccgcgtg
     1621 cagcaccggc tcttcgttcg tcaggcaatc gacgttgggg gccaacttgt cgccgaactg
     1681 gcgcgcgtgc ttcggctccc ccaggcccag ggcgacgatg cgcagcccgg ccgcttcaat
     1741 ttcctctcgg tgcagctcta actgagccag ccattcgcgg cagtggattc agccaaagtg
     1801 ccgcaagaag gtcagcaaca ccggcccctc ttgccaaaag gtcgatagct gctgctcctg
     1861 cccgcttttg tccagacagc ggccgtccgg ggcagtgtcg cccggggcca gctttttctc
     1921 tttggtcatg tcgattgtgc tcccggtgcg ctttcaatga gcacaccatt accccctaaa
     1981 cttatcagat tgcacataaa atgtcaatat tttgtcacga atctagcgat aacacttcgc
     2041 agccggggcg gctggggtgg gcgcgcacgg gcgtggccgg cggcaggccg cgcatggccc
     2101 aaagggcaac caggtcgccg ctacaggcga cgatcatcca cagcccccac agcaccagca
     2161 gccacgagcc agcgacgatg gcggccacgc cgggcagaac gcccagcagc agcgtcggca
     2221 atagcaggat gagccgatag gcgcgggccg gcacaggcgc gtcgcagcgc acgcgcgggc
     2281 cgagggcagc atggccggcg gccaggcggg ccgcgccacg cggcacgcgg ccgatccagc
     2341 gatagccggc cagtagcacc gcttcgcgca gcaacagccc gcccagcaac agggcgaaca
     2401 ccgccagcag cgccgtcacc ggccagcgct ccggcagttg cggaggcgtc aggccccagc
     2461 gcagttggaa gggaatgaac agggccatgg ccgccacggg gatcgtgccg aaggcgagca
     2521 tcatggctac gggcagcggc acagtgcgct cagtgacggc gcgggggctg ggcggggatg
     2581 gcgcgtcggc cagggccgcc accggggcca cgtcgatgcg cgtgccttcc gccagggtgt
     2641 cctccaatga cagagcgtgg ccgaagaagt ccagcatctg ccgctcatac gccttcggcg
     2701 cgacgaccca gcccatggcg tgggcggcgt cggggatctc gtagagcgtt ttcggctcgg
     2761 cggcggcggc gaagaagtgg cgcgttagcc gctgctccag gccgcgaccg gtgctgatga
     2821 gcaggacggg ccgcccgccg aggcgggcca gggcctgcat gttggccggc ggacgcgggc
     2881 cgggcgcgaa ccagtcgatg gcccgctggt agccgcgctc gcgcgggaag cgccacagcc
     2941 gttcgaacca gccgcgcggc ggcggcaggt cgtcgattgt gcccaggagc ggcccatcgg
     3001 ccatgacggc gcggatgaag acattgtgcg ccgcggcctg gatggccagc attccgccca
     3061 cggagatgcc cagcacgcca acgcgggcct gcacgtcgcg gcggcgcgac agccaggcca
     3121 ccgccgccag cacgtcatcg accccccgct ccccccgcga gaaaggccgg ccgccgctct
     3181 ggccatgggc gcgcaggtcg aacagcagca cgccatagcc cgcccgcgcc agcgtcgcgg
     3241 cgtggaaggc gacgctgagc cggttgccgc cgtggccgtg gagcaggacg acggccgtgc
     3301 cgttgcgcga ggggatgtac cagccccgca agggcgcgcc gtcgccgccg gcgaactcca
     3361 cgtcctcgta gtccgcgccc caatcggccg gcgtgtgccc ggacaggggg cggcgcagcg
     3421 gccggctgaa ctgccaggcg agcagcagcg gccggagcag caagtgaagg gcaacgagaa
     3481 tgagaatgac ttccaggatc atatcaggct cacggtgggc agcgctgcgt tctattgtaa
     3541 ctcacgttgc ccctgttgcc gccccgccgt ccatcggcta cacttcgccg ctatgcgcat
     3601 ccgtgccgcg cggctactgc ttcctctggc gttcctggtt ctccttgttg ctttcgtcat
     3661 catctggcgg gcgcgctcgc agggcgtctt cggcccggcc gtggctctct gccccggccc
     3721 ggatcgctat ggctacacct gcgaaggcgg cgccgcctat gccaccatcg acgccgccga
     3781 cgccaccggc ctgctggccg acgacgccgt catcaccctg gccttgccct tcccgttcac
     3841 cttctacggc gcgacctata cggacgtgaa cgtgtccacc aacggcaacc tgcaattcac
     3901 gaccgacaat ccgctgcccc ggcccgactg cctggccccg gcggccggca tgggcgacct
     3961 gatcgctccc tattgggccg acctggacct gaccgcccac ggcgcgctgg agacggccgt
     4021 cgttggcgaa gcgccggcgc gcgtcttcgt cgtcgagtgg gacgacgtgc ccctctatgg
     4081 cacggacagc agcgaccggg tgacgttcga ggtgcaactg ttcgagacga gcaacgacat
     4141 tgtcttcctc tatgaggacc cgacgacggc tggcggcggc aacggcggcg cggccgtggc
     4201 cggcatccag agcgagcggc aaggcctctc gctgagctat agctgccgcc agccggtgct
     4261 acccgcgccg ggcggcctgc gtctggcgca tccggccgag ccgaaccgcg acgccggcca
     4321 gccggaggac gccccggccg cagttggccc ggccatgagt ggcccggccg ccgccgcgcc
     4381 gccggccaag gggccgctgg ccgagttgct ggccgcctat gagacgcgcg gcccggcggc
     4441 gttggcggca ttacaacgcc agtgggccgg cggccgcccg gcgcgggcct ttgtctggcg
     4501 cgccgccgac ctgaccggcg acgggacggc cgagctggtc gctgcctggg gcggcgggcc
     4561 gggcgcgccg gaactgaccg aggtggctgt gctggccgtg ggcgcggggc agccgccggc
     4621 gctgctgttc gaccgtcgcc tgagcacgcg cgcggccggc tatgcggctg taaacgtggc
     4681 cgaggtggct gacctgaccg gcgacggcgg cgacgatgtc gtcctgcgcg atcagaagac
     4741 gggcacaacg tgggttctga cgtgggcgct gccggccgcc gcgggcgagc cggcgctgct
     4801 ggacgtgccc gcgcgctgcg gtggtgatct gatcattcgc gacaccgacg gcgacggccg
     4861 cctcgaactg atccgcgacg gctgcgaccg gcccggccgg gtcagcgtgg cctgggacgg
     4921 cgcggccttt gtgattgcgc cctgagacga agcgcttgcc ggcgcgtagt gcccttacgg
     4981 gcatggggcc tgactgggcc gcggccccat ttgcaccccg tgcacttaac agatactatg
     5041 cggcccaaat ttgcacagga gtaaaaggta gcagcgatgt tcgaactcaa cggggtttat
     5101 cgaaatcgca tcggtgaata cacagtcgtg gccctgaacg gcccacgcat ggtcgtgcgc
     5161 tatgccgatg ggacggaagc ggagttgaac gtcaatatcc aggcccgcat ctgggagaat
     5221 attgtcgccg agcaagaggc gcgggcggcc agtagccgcc atctgcgtca gtcgaacaag
     5281 gatacgaccc actacatcaa ggccgtcagt ctgccaccgg gcgaggaact ggccttcccc
     5341 ggctggcagg agcgggtcgt catggccccg acgccggaac tggcccaacg tctgaagtcg
     5401 ggcgaccgct ttatctacta tgccgttgag gcacaaacgt tcttcgccgt cgtcactatc
     5461 accggcgagg tcttccaggc cgatcccaag cagcacacct tcaccgtcga ggccgacaag
     5521 gcgttcttct tcccgaccga tgtcgatgcc gcctcggcca acctggagca tggcgtcagc
     5581 gccgactcta tcgaactaga gagctatcct gacttcggca agctccacat cgagccggag
     5641 tccttcttcc gcatcagcga ggacgatttc gaactgctgg ctgaagccct gacggagatc
     5701 accgaagacg aggaagagga aaccgaggaa ttcgaagagt acgaggagga cgaggttgaa
     5761 taaggcccag tccgaaaaca tcctggtttg catcgcctgg ccctatgcca attcggacat
     5821 tcatcagggc aacgtcaccg gctcccacct gcccggcgac atctacgcgc gcttccagcg
     5881 cctgcgcggc caccatgtcg tcatggtcag cggctccgac agccacggca cgcccgttac
     5941 ggtcaaggcc gaggagttgg gccgcagcaa cctggagacc tacgaactct atcacgcccg
     6001 cttcctggaa ctatttcgcc gcctgggcat cagctacgac ctgttcacca gcacccatac
     6061 ggagaaccac ttccgcgtgt cgcaggacat gtttctgcag ttgctgaaga acgagtacct
     6121 ctaccgcaag gtgacgccgc agatgtattc gccggaggcg ggccaattcc tgcccgaccg
     6181 ctacgtggag ggcacgtgcc ccaattgcgg ctacaccggc gcgcgcggcg accagtgcga
     6241 caactgcaat acgctcttcg agagcgccgc cgagttgctc aacccgcgca gcaagcgcga
     6301 caacacctcg ctggtgctgc gcgacacaga acactttttc attgacctgc cggccattgc
     6361 cgccggcgcg ctgtccgagt cgatccacga cgacaagcag cactggcggc cgcaggtggt
     6421 caacttcgct cgcaacttca tcgaccaggg gctgatcggc cgggctgtca cccgcgatat
     6481 ggactggggc atccctgtgc ccgtggacga aggctacgac gagaaggtac tctacgtctg
     6541 gttcgaggcg gtcatcggct acctctcggc taccattgag tgggcccaca accaggacga
     6601 caatgacgcc tggcgcgact ggtggcagaa cccggacgcg cggacgctct acttcatcgg
     6661 caaggacaac atccccttcc acaccgtcat ctggccggcg caacttctgg gcgcgcgcgg
     6721 cctctacagc gataacccgc aggcgacgct gaatctgccc tacgacgtgc ccgccaacga
     6781 gttcatgaac atggaggggc ggaagatcag cggcagccgg aattggggcg tgtggatgct
     6841 cgacgcgctg gatcgctacg accccgaccc gctgcgctat tacctgacaa ccaacatgcc
     6901 ggaggcgcgc gacagcgatt ggacttggag cggcttcatc gagcgcaata acaacgagtt
     6961 ggttgccaac tggggcaacc tggtcaaccg cgtgctcaac atgatccggc gctacttcgg
     7021 cggcgtggtg ccggagccgg gcgcgctgac cgcggccgac gaggccctgc tggccgccat
     7081 cgacgacggc ttcgacagcg tggccacgtt ctacgacggc tgcaagttcc gcgccgctgt
     7141 gcaggaagcg ctacgc
//
LOCUS       DAOSMQ010000108         7134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10534_length_7134_cov_4.799548, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000108 DAOSMQ010000000
VERSION     DAOSMQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10534_length_7134_cov_4.799548"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK829_05120"
     CDS             <1..488
                     /locus_tag="PK829_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40628.1"
                     /translation="SGLPVTVATCVIASIMFGLFISAIVPSQDVVLYIILVQLFVQII
                     LSGTLFPLGDSAGAKLASKMVISHWTMDALGSSVDLPGLDEDKSVACSAVWLPANPQL
                     GTTEPTTTVECVPAPLGDKLSLDYRNSEKHLAATWLALGGMALFWGVLTVLVQRRKQV
                     D"
     gene            593..1270
                     /locus_tag="PK829_05125"
     CDS             593..1270
                     /locus_tag="PK829_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOU40629.1"
                     /translation="MTTRQASATTAEPQRLRMSYAEYIAWADEDVHAEWVDGEVIVQM
                     PPYDVHQIVLNFLNHLLHLYVEFQELGVVLIAPFEMRLLSEKSARQPDILVVTSENLG
                     RLSRERLEGPADLVVEIVSRDSVRRDRHDKLHEYRRAGVREYWVVDPRPGRNRADFYR
                     LDAAGEYELYATDEDERVTSAVLPGFWLRPAWLWLESKPPVMTAFLEILSPEQRERFR
                     RLVAGEG"
     gene            complement(1250..2443)
                     /gene="mutY"
                     /locus_tag="PK829_05130"
     CDS             complement(1250..2443)
                     /gene="mutY"
                     /locus_tag="PK829_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008315009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOU40630.1"
                     /translation="MCAAEEGGGAGEKESRGEESPLLPRSPAPLLPLSPFPLLAWWDA
                     NRADLPWRRTGDPYAIWIAEVMLQQTQIAAVIPYYERWLARFPTVEALAAAPLDDVLK
                     QWEGLGYYSRARHLHTAARRIVADHDGRLPDTLAELLRLPGVGRYTAGAVASIAYGVR
                     APVLDGNVIRVLSRLFDVDDDVTRPETRARLWDIAEALVPAERPGDFNQALMELGQVI
                     CVPQRPRCLLCPLRDDCLARQRGTQLERPVRPPRRRTPHYDVVAVIIWRDGRPAADGH
                     FLIAQRPPEGLLGGLWEFPGGKVEPGETRPEALRREIAEELALAIVPGDFLIEVAHAY
                     THFRITLHAFHAAYTGGEPQHLGVADHAWVTLADVERYAFAVTDRKIIQALRALFAPS
                     ALPGD"
     gene            complement(2430..3434)
                     /locus_tag="PK829_05135"
     CDS             complement(2430..3434)
                     /locus_tag="PK829_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU40631.1"
                     /translation="MKPLQRFETSAGRAIFSFPVRSFPTLTNNIYVIDDGEQYILIDA
                     GSGMERPNADLLAGFTAIGEHLGRPFTPADIGTILITHGHIDHFGGLPFLRQYTAAPA
                     GVHILDRRVLTNHEERMVFAYRRLETFLEGAGVPAEERVVLMSVYLFSKTYYRSTPVQ
                     FLLAEGQPTVGGFGVFHVPGHCPGQVCLLVDDVLLTADQVLARITPHQAPESITMNMG
                     LGHYLDSLDKVAGLPGVRLGLGGHEDPIDDVPGRVAAIRAAHDERLGKIMDICRAPQS
                     IAGISRALFGHVDSYHVLLALEETGAHVEYLHQRGELMAANLEEIEATSRPVVEYVRG
                     "
     gene            complement(3470..4747)
                     /gene="kynU"
                     /locus_tag="PK829_05140"
     CDS             complement(3470..4747)
                     /gene="kynU"
                     /locus_tag="PK829_05140"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HOU40632.1"
                     /translation="MKGRDADLPLRRARERDAADPLTAFRDRFVLSDPELIYLDGNSL
                     GRLPRATAPRLAAMVEDEWGGRLVRGWNEGWFDAPDRIGGKLAGLLGAAADEVILADA
                     TSVNLFKLSLAALLARPGRHTIVTDDLNFPSDLYILRSAAALAGRPVAVRVVPSADGI
                     HGPTAALAAALNEDTALLSLSHTVFKSGYTYDMAALTALAHRAGALALWDLSHSAGAV
                     VVDLHGAEADLAVGCTYKYLNGGPGAPAFLYVRRDMQDMLANPISGWLGRADPFDFAL
                     DYAPAAGIRRFLTGTPPILSLAAVEPGIDLLLEAGMARLRERSVRLSEYLIGRWQERL
                     KPLGFRLNSPRDPAQRGSHISLGHPDGLRINMALIHEAQVLPDFRRPDNIRLGIAPLY
                     NTFAELHTAVERLRAVVVEGLYRHYDAAGVAVT"
     gene            complement(4791..5432)
                     /locus_tag="PK829_05145"
     CDS             complement(4791..5432)
                     /locus_tag="PK829_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOU40633.1"
                     /translation="MTTKPRLYDISRDLRNGMAVWPGDKDFALTPNGSIAEGGPVNVS
                     TLFLSSHSGTHVDAPYHFEDDAATLEQVDLSAYWGLAQVVTVTKEAGPLVPADFEGYD
                     LSLAPRLLVHSALSAADMAVFHEEFVYPSPELAGYLGQLGVVLYGADSPSMDAFDSKD
                     LAGHHALRRNNILILEGLLLAGVPDGLYELSAFPLKLIGGDGSPVRAVLRTLE"
     gene            5623..5943
                     /locus_tag="PK829_05150"
     CDS             5623..5943
                     /locus_tag="PK829_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40634.1"
                     /translation="MSSRTFWFLLIAAVVVGLAIAWVDSRPTWDDTGITAGVVLLTSA
                     VFGFVAPRRPWLWALAVGGMIPLLGILRGGNFGSLLALVVALAGAYGGALVHRAMYGT
                     IAAK"
     gene            5952..>7134
                     /locus_tag="PK829_05155"
     CDS             5952..>7134
                     /locus_tag="PK829_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOU40635.1"
                     /translation="MRLSLVWLAAGLALAGAALTLLAQGAGRSYAPVIILGPPSSGQL
                     VVKINFQPAAAAVPPGYLPDSGAVYANRGNGFAYGWNADNSAATRERNSPDAYDQRYD
                     TLIHLQKPENPDAVWELALPPGVYDVYAVAGDPAASDGFMHSTAEGQTLLSGAPGDNH
                     RFIAWTLSVPVTDGRLTVRSGSSAVNNKLLFLEVYRTATLPTPSPTPTRPPGMVELRG
                     LWVTRFDWTVFNRPADPARIDQIVANAADAGFNAIFFQVRGTADAYYTPGLEPWAARV
                     SGGVLGQAPAPLWDPLAYFVERAHARGLQLHAYVNVYPIGDRTAANTCPPPPAVNPTP
                     LWYRLRDAHGLTNDKLNAAQWTTLDAVSCGEYQVASPASAVFREHFKAVARDLVSRYD
                     ID"
BASE COUNT         1125 a         2324 c         2496 g         1189 t
ORIGIN      
        1 gctccggcct gccagtcacc gtggcaacct gcgtcatcgc cagcatcatg tttggcctgt
       61 tcatctcggc tattgtgccg tcgcaggacg tggtgctcta catcatcctc gtgcagctct
      121 tcgtgcagat cattctctcc ggtacgctct tccccctggg cgacagcgcc ggggccaagc
      181 ttgcctcgaa gatggtcatc tcccattgga cgatggacgc gctcggctcg tcggtggact
      241 tgcctggcct ggacgaggac aagagcgtgg cctgctcggc cgtgtggctg ccggccaacc
      301 cgcaactggg cacgaccgag ccgacaacga cggtcgaatg cgtgcccgcg cccctgggcg
      361 ataagctcag ccttgactat cgcaacagcg agaagcacct ggcggcgacg tggctggcgc
      421 taggcggcat ggccctcttt tggggcgtgc tgacggtact agtgcaaagg cgaaaacagg
      481 tggattagcg ttcgaccact tcgccgcctc ccgcgccggg ctacactgag cctatgctat
      541 actgtgtctg gctgatgatc tgggctggtt cgcctttcgt aggagatagc gcatgaccac
      601 aagacaagca agcgcgacga ccgctgagcc gcaacggctg cgtatgagct acgccgagta
      661 tatcgcctgg gcggatgagg atgttcatgc cgagtgggtc gatggagagg taatcgttca
      721 aatgccgccg tatgacgttc atcaaatcgt cctgaatttc ctgaaccacc tactacacct
      781 gtacgtcgag tttcaggaac tcggtgttgt gctcatcgcg ccgtttgaga tgcgcttact
      841 gtccgaaaag tcggcccgcc agcccgatat cctggtcgtc acgagcgaga acctcggccg
      901 gttgagccgc gaacgccttg aggggccggc cgacctggtg gtcgaaatcg tctcgcgcga
      961 cagtgtgcgc cgcgatcgcc acgacaagtt gcacgagtat cgccgggccg gtgtgcgcga
     1021 gtattgggtg gttgacccgc gtcccggccg caaccgcgcc gacttctacc ggttggacgc
     1081 tgccggtgag tacgaactgt acgccacgga cgaggacgaa cgggtcacct cggcggtgct
     1141 gcccggcttc tggctgcggc cggcgtggct ctggctagag agcaagccgc ccgtcatgac
     1201 cgccttcctg gagatcctct cgccggagca gcgcgagcgt tttcgccgtt tagtcgccgg
     1261 ggagggctga gggcgcgaac agcgcccgca acgcctggat gatcttgcgg tcggtgacgg
     1321 cgaaggcgta gcgctctacg tccgccagtg tcacccaggc atggtcggcc acgccgaggt
     1381 gttgcggctc gccgccggtg taggcggcgt ggaaggcgtg gagggtgatg cggaagtggg
     1441 tataggcgtg ggcgacttcg atgaggaagt cgcctggcac gatcgccagg gccagttcct
     1501 cggcgatctc ccgccgcagc gcctccggcc gcgtctcgcc cggctcgacc ttgccgccgg
     1561 ggaactccca cagcccgccc agcagcccct cgggcggccg ttgggcgatg aggaagtggc
     1621 catcggccgc cggccgcccg tcgcgccaga tgatgaccgc gacgacgtcg tagtggggcg
     1681 tgcggcggcg cggcgggcgc accggccgct ctagctgcgt gccgcgctgc cgggccaggc
     1741 agtcgtcgcg cagcgggcag agcaggcagc gcggccgttg cggcacacag atgacttgcc
     1801 ccagttccat cagcgcctgg ttaaagtcgc ccggccgctc agccggcacg agagcctcgg
     1861 cgatatccca caggcgcgcg cgcgtttccg gccgggtcac gtcgtcgtct acgtcgaaca
     1921 ggcggctgag gacgcggatg acgttgccat ccagcactgg cgcgcgcaca ccgtaggcga
     1981 tggaggccac ggctccggcg gtgtagcggc cgacgcccgg cagtcgcagc aactccgcca
     2041 gcgtgtccgg caggcggccg tcgtggtcgg cgacgatgcg ccgggcggcg gtgtgcagat
     2101 ggcgcgcccg gctgtagtag cccaacccct cccactgctt gagcacatcg tccagcggcg
     2161 cggcggccag cgcctcaact gtggggaagc gggccagcca gcgctcgtaa tagggaatga
     2221 ccgcggcgat ctgcgtctgc tgcaacatca cttcggcgat ccagatggca taggggtcgc
     2281 cggtgcgccg ccagggcagg tcggcgcggt tggcgtccca ccaggcgagc agggggaaag
     2341 gggagagagg gagcaggggg gccggggagc gggggagcag gggggattct tctcccctgc
     2401 tctccttctc ccctgctccc ccgccttctt cagccgcgca catactccac caccggccgg
     2461 ctcgtcgcct cgatctcttc caggttggcg gccatcagct caccgcgctg gtgaaggtat
     2521 tcgacgtgcg cgcccgtttc ttccagcgcc aggaggacgt gatagctgtc cacgtggccg
     2581 aagagggcgc ggctgatgcc ggcgatggac tggggcgcgc ggcagatgtc catgatcttg
     2641 cccaggcgct cgtcatgggc ggcgcggatg gcggcgacgc ggccgggcac gtcgtcgatg
     2701 gggtcctcgt ggccgcccag acccaggcgc acgcccggca gccccgccac cttgtcgagc
     2761 gaatcgaggt agtggcccag gcccatgttc atggtaatgc tctccggggc ctggtggggc
     2821 gtgatgcgcg ctagcacctg gtcggccgtc agcaggacgt catcgaccag caggcagacc
     2881 tggccaggac agtggccggg cacgtggaac acgccgaagc cgcccaccgt cggctggccc
     2941 tccgccagca ggaactgcac cggcgtggag cgatagtagg tcttggagaa caaatagacg
     3001 ctcatcagca cgacccgctc ctcggccggc acgcccgcgc cctccaggaa cgtctccagc
     3061 cggcgatagg cgaagaccat gcgctcctcg tggttggtca gcacgcgccg gtcgaggata
     3121 tggacgccgg ccggcgcggc ggtgtactgg cgcaggaagg gcaggccgcc gaagtggtcg
     3181 atgtggccgt gggtgatcag gattgtgccg atgtcggccg gggtgaaggg gcggccgagg
     3241 tgctcgccaa tggccgtgaa gccggccagc aggtcggcat tggggcgctc catgccggag
     3301 ccggcgtcga tgaggatgta ctgctcaccg tcgtcgatga cgtagatgtt gttggtcagc
     3361 gtggggaaag agcgcactgg aaaagagaag atggcccgtc cggcgctggt ctcgaagcgt
     3421 tgcaggggtt tcatggtggt tgtttttcgc tgccgagggt ttgggtcgct caggtgacgg
     3481 ccacgccggc cgcgtcatag tggcggtaga ggccctcgac gacgacggcg cgcaaccgtt
     3541 cgacggccgt gtgcagttcg gcgaaggtgt tgtagagcgg cgcgatgccc aggcgaatgt
     3601 tatccgggcg gcgaaagtcg ggcaagacct gcgcctcgtg gatgagggcc atgttgatgc
     3661 gcaggccgtc ggggtggccc agagagatgt gagagccgcg ctgggcaggg tcgcgcggcg
     3721 aattaaggcg gaagcccagc ggcttcaggc gctcctgcca gcggccgatg agatactcgc
     3781 tcagccgcac cgagcgctcg cgcagccgcg ccatgcccgc ttccagcagc aggtcgatgc
     3841 ccggctcgac ggccgccagc gacaggatgg gcggcgtgcc tgtcaggaag cggcggatac
     3901 cggccgcggg ggcgtagtcg agagcaaaat cgaatgggtc ggcccggcca agccagccgc
     3961 tgatggggtt ggccagcatg tcctgcatat cccggcgcac gtagaggaaa gccggcgcgc
     4021 cgggaccacc gttgagatac ttataggtgc agcccacggc caggtcggcc tccgcgccat
     4081 gcagatcgac gacgaccgcg ccggccgagt ggcttaggtc ccacagcgcc agcgccccgg
     4141 cccggtgggc cagcgcggtc agggcggcca tgtcataggt gtagccgctc ttgaagacgg
     4201 tatgggagag gctcagcagg gccgtgtcct cgttgagggc ggcggccagc gccgcggtcg
     4261 ggccgtggat gccatcggcc gagggcacga cgcgcacggc caccggccgc ccggccagcg
     4321 ccgcggcgct gcgcaggatg taaaggtcag acgggaagtt gaggtcgtcg gtgacgatgg
     4381 tgtgtcgccc cggccgggcc agcagcgccg ccagactgag cttgaacagg ttgaccgagg
     4441 tggcgtcggc caggatcact tcgtcggccg cggcccccag cagaccggcc agcttgccgc
     4501 cgatgcggtc gggcgcgtcg aaccagccct cattccagcc acggacgaga cgcccgcccc
     4561 actcgtcctc caccatggcc gccagtcgcg gcgcggtggc ccgcggcagg cggccgagcg
     4621 agttgccgtc cagatagatc agctccgggt cggagaggac aaagcggtcg cggaaagcgg
     4681 tcagcgggtc agccgcgtcc cgctcgcgcg cccgtcgtag gggcagatct gcgtctctgc
     4741 ctttcatcct tcattcctca tccctcatcc ctcatccctc cgccacgcgc ctactccaac
     4801 gtccgcaaca cggcccgcac cgggctgccg tcgccgccga tgagcttcag cgggaaggcc
     4861 gacagttcat agagaccatc gggcacgccg gcgagcagca ggccctccag gatgagaatg
     4921 ttgttacgcc gcagggcgtg gtggccggcc aggtctttgc tgtcgaaggc atccatcgat
     4981 ggcgaatcgg ccccgtagag gacgacgccc aactggccca gatagccggc caattccggg
     5041 ctgggataga cgaactcctc gtggaaaacg gccatgtccg cggcgctgag ggcggaatgc
     5101 accagcaggc gcggcgccag gctcaggtca tagccctcga agtcggccgg caccagcggc
     5161 ccggcttcct tcgtgacggt gacgacctgg gctagacccc aataggccga cagatcgacc
     5221 tgctccagcg tcgccgcgtc gtcctcgaaa tggtaggggg catcgacgtg cgtgccagag
     5281 tggctgctga gaaagagggt cgagacgttg accgggccgc cctcagcaat gctgccattg
     5341 ggggtcaggg cgaaatcctt gtcgcccggc cagacggcca tgccattgcg gaggtcgcgg
     5401 ctgatgtcat acagtcgagg tttggtggtc atgctactcc ttctcgtggg tctaaatcct
     5461 ttgggcgagt ataaaccagc ggggatagct gtcaatcagg cgacggggcc ggggtctgag
     5521 aggttttgac cgttccgtgc tggcggcgcg tgctatgatg tttcctgtgg cgcggcgggt
     5581 gggccgctgc caacgaaatt tcctgctcga cggagagaaa agatgtcatc acgtaccttc
     5641 tggttcctgt tgatcgcggc tgtggttgtg gggctggcca ttgcctgggt ggacagccgc
     5701 ccgacgtggg acgacacggg catcaccgcc ggggtggtgc tgttgacgtc ggccgtcttt
     5761 ggcttcgtcg cgccacggcg gccgtggctg tgggcgctgg ctgttggcgg catgatcccg
     5821 ctgctgggca tcctcagggg cggcaacttt ggcagtctgt tggcgctggt tgtggctctg
     5881 gccggggcct acggtggggc tttggttcat cgagccatgt atggcacaat agcggctaaa
     5941 tgaatcgctc ggtgcgtctt tcgcttgtat ggctggcggc cggcctggct ctggccggcg
     6001 cggcgctgac gctcctggcc cagggggcgg ggcggagcta tgcaccggtc atcatcctcg
     6061 gcccgccatc gagcggccag ctggtggtga agatcaactt ccagccggcc gcggcggccg
     6121 tgccgcccgg ttacctgccc gacagcggcg cggtctatgc caaccgcggc aacggcttcg
     6181 cctatggctg gaatgccgac aacagcgccg ccacgcgcga gcgcaactcg cccgatgcct
     6241 atgaccagcg ctacgacacg ctcatccacc tgcagaagcc ggagaacccc gatgccgtct
     6301 gggagttggc cctgccgccc ggcgtctacg atgtctatgc cgtcgctggc gatccggcgg
     6361 ccagtgacgg ctttatgcac agcacggccg agggccagac actcctttcg ggcgcgcccg
     6421 gcgataacca tcgcttcatc gcctggacgt tgagcgtgcc cgtgaccgac ggccgcctga
     6481 ccgtgcgcag cgggtcgtcg gcagtcaaca acaagctgct ctttctggag gtctatcgca
     6541 cggcgacgct gccgacgccc tcgccgacgc ctacgcggcc gccgggcatg gtcgagttgc
     6601 gcgggctgtg ggtgacgcgc ttcgactgga cagtcttcaa ccgcccggcc gacccggcgc
     6661 gcatcgacca gattgtcgcc aacgccgccg acgccggctt caacgccatc ttcttccagg
     6721 tgcgaggcac ggccgacgcc tactacacgc ccggtctgga gccgtgggcg gcgcgggtca
     6781 gtggcggcgt actggggcaa gcgcccgcgc cgctatggga tccgctggct tactttgtcg
     6841 agcgcgccca cgctcgcggc ctgcaactgc acgcctacgt caacgtctac cccattggcg
     6901 accgcaccgc ggccaatacc tgcccgccgc cgcccgcggt taaccccacg ccgttgtggt
     6961 accgcctgcg cgatgcccac ggcctgacca acgacaagct caacgccgcc cagtggacga
     7021 cgctcgatgc cgtctcctgc ggcgaatatc aggtggcttc gcccgcctcg gccgtcttcc
     7081 gcgaacactt caaggctgtc gcccgcgacc tggtcagccg ctacgatatc gacg
//
LOCUS       DAOSMQ010000109         7115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10572_length_7115_cov_4.638102, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000109 DAOSMQ010000000
VERSION     DAOSMQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10572_length_7115_cov_4.638102"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PK829_05160"
     CDS             complement(<1..672)
                     /locus_tag="PK829_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40636.1"
                     /translation="MTVACPTATDDTYLLRNPQHGYCLLYPATHKVERPNADEVDLVV
                     GSLLNAGDPRANIRVMTGNDLSAGAFADDLVAGFEGFDIARSEMTVAGQPAVVLDDVP
                     GQDINRQVIFTLDGRLYHIYFAPVDPANTAPIDDFMAVILDSFTPMPVSETLLPEDEC
                     LAAKSEQTTVRSDAFGFCALVPADFTYEETSAANANLFIGSMMDVAHPKLMIEVTDAG
                     GQTATA"
     gene            1171..2136
                     /locus_tag="PK829_05165"
     CDS             1171..2136
                     /locus_tag="PK829_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOU40637.1"
                     /translation="MADIQATANRIVENVERVIVGKRQPVQLTVLGLLCQGHILIEDV
                     PGVGKTVLAKSLAKSVGCKFQRIQFTPDMLPSDVTGVSVFNQGTREFEFRPGPIHAQI
                     VLVDEINRATPKTQSALLEAMEERQVTVDGRTYPLDVPFMVLATQNPIEYEGTFPLPE
                     AQLDRFMLRIRLGYPGKEEEIEILDRQQHAHPINALQQVVDVDELLAAQQAVKDIYVD
                     PLVKEYIVNLVRQTRDHPDVYLGSSSRGALAIYRLGQARAALMGRDYVLPDDVKALAG
                     PALGHRIIVGPAARIKDTEPEQIVQDLLDNMAVPGAHVGRQRTRV"
     gene            2136..3437
                     /locus_tag="PK829_05170"
     CDS             2136..3437
                     /locus_tag="PK829_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU40638.1"
                     /translation="MSGFLGQRRTVVFALAVVAFIAGLITGRDLLFTLAYLLGLLLIL
                     SFLWAWVNLNGVHLSRVTRVRRTQVGRPLEERFSVRNTTVVPKLWLEVRDYSTLPYHY
                     SSHVVNGLGGRQNYSWRVTTICQQRGRYQLGPIRLRTSDPFGLFPLERELTPTSNVVV
                     FPMTFPIHQFALPLGVLPGGDALRRRTHYITTNAAGVRDYEPGDSFNRIHWRSTARRD
                     RLVVKEFELDPLADIWIVPDMSVYGHVTPRKDAVPPPPPGDIPGWLKMQEFQLPESTE
                     EYAVSIAASVAQYFLRRDRAVGMLAYGQSNEILQPDRGERQFNRILETLAVLRAEGDV
                     PLQDMLHTESHLFPRGTTIIAVTPITREEWSVAARQLARRGLRVVTILVNPASFHGSR
                     SAAPLYQLLRGGGQVAYMVNNGDDLTTALSSGSTQAGFFTV"
     gene            complement(3487..4902)
                     /gene="miaB"
                     /locus_tag="PK829_05175"
     CDS             complement(3487..4902)
                     /gene="miaB"
                     /locus_tag="PK829_05175"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU40639.1"
                     /translation="MTKTYHFWITGCQMNYADARRVATELEKLGYRPTPRAEDADVVV
                     LETCTVRQQSEDKAYHKLQSLRPLKARRPDMTVAVMGCVVGVRGNEALTQRFPFVDVF
                     MEPSTDGLPLVSHLTQDSDYALELAETAARHALMDGEDAGKITLAPLPPRSPAPPLPI
                     SAPVAVVYGCSHACTFCIIPQKRGRERSRPVGEIVAEARGLAARGVKEVVLLGQIVDR
                     YGTDIPDGPDLADLLRVVNDVDGLERIRFLTSHPNYMTDRILRAVAELPKVMPQIEVP
                     IQAGDNDVLEAMKRGYTREQYRELVQHVRAVIPDAAIHCDVIVGFPGESEAQFMATYD
                     ILAELRLDKLHLARYSPRPGTVSARRMADDVPDEEKRRRFHMIEALQKEVSTEKMRRY
                     LGQTVEVLVEERDKGRWRGRTPHNKLVFFDDAGDLRGQLVAVHVTHTGPWSMSGKKVD
                     DGPQTPAHRPAGESIPLTLAM"
     gene            complement(4959..>7115)
                     /locus_tag="PK829_05180"
     CDS             complement(4959..>7115)
                     /locus_tag="PK829_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40640.1"
                     /translation="NEAIVTVDQPYRRDDGGTDLLQQTTVFRRGDRRWLLAPPVGDFW
                     GEWQSSEGDYLNLIYPERDAVIAEQLAIDLDAAIAGMCATLADIDCSADLFLTVRLDT
                     EPQTLADLSQPIGALRRARQTANILELPAPSLVGLPAGDEGERKAAYAALRDGYARHV
                     LGAAIAQAVSWRCCDASLLFSMLLPYQLVDLGLVSAPPVGPDDYRRVLDERIPMQRLN
                     LLTNERLPAEIDPDRLWLARVAVDFLLHAGDLSPADLQRRLSSVRSFNQFAQQVLQDG
                     AAGRPVPADLTQALWLYAIQRAAEAESEAPLPDETLYLVCTAPGEAQTPETSQLLRYL
                     PATGDWEHLYSVQGFIWMTPLPGWETMLLQQYTVATDTWQSRYWRAGEIATAYTTDDP
                     SSAISLGQSDAAGTRLVIYQRDTLGNDIRALVTDLTACEGNTCATEASPGQPLWSPNG
                     QWALYQGDGSENPGPQLVVNGRYLLLDSNEPFAVMPIALGTAGDSGRPVILGQGYAPF
                     WIDERTFGFIRPVGGATSTTDGGEEIVVATVDDSTPQTLLLSADLFQFLPHDSRMRQM
                     NLAYVAPNPSQPDSLFIVARDEVAEDAYVFLFDRATGQIELRLTLGADFNQSLGFSPD
                     GRYLVLTGVGSMSYTGGSNLAPMLLHDIAANRTIPLLSRLPYFLPSVTYDWTRDGRLI
                     VALDDNLLGLVIPDTGQVRLLRHNAGACTSVAWLDE"
BASE COUNT         1159 a         2299 c         2407 g         1250 t
ORIGIN      
        1 cgccgtggct gtctggccgc cggcatcggt cacttcgatc atcagcttgg gatgggccac
       61 gtccatcatt gagccgatga acaggttggc gttggcggcg ctggtctctt cgtaggtgaa
      121 atcggccggc accagggcgc agaagccgaa cgcgtcgctg cggacggtcg tttgttctga
      181 cttggccgcc aggcattcgt cttcgggcag gagcgtctcg ctgacgggca tgggcgtgaa
      241 gctgtccagg atcacggcca tgaagtcgtc gatcggcgcg gtgttggccg ggtcaacggg
      301 cgcgaagtag atgtgataga gccggccgtc gagcgtgaag atgacctgcc ggttgatgtc
      361 ctgcccgggc acgtcgtcga gcaccacggc cggctggccg gccacagtca tctccgagcg
      421 agcgatgtcg aagccctcga agccggccac cagatcatcg gcaaaggccc cggccgagag
      481 gtcattgccg gtcatgacac ggatgttggc tcgcgggtcg ccggcgttga gcaggctgcc
      541 gacgaccaga tcgacctcat cggcgttagg ccgctcgact ttgtgggtgg ccgggtaaag
      601 caggcagtag ccgtgctgcg ggttgcgcag caggtaggtg tcgtcggtcg ccgtggggca
      661 cgcgacggtc ataggtgtct cggccgcgcc gggggcgtcg gtcgccgcgg gggcgtcggt
      721 cgccgcgggg gcggcgggtt ccggagcgtc ggttgtggcc gctggcgcct gcgtcccagc
      781 ggtggggcta caggcggcgc caagcagcgc caatagaacg agaatgatag ctattgcgcc
      841 aattcgcttc atcaatggtt tactcccttg gtgattatct ttggccagag ctttaggaac
      901 cagatcgctg tccggccgct ctgcgcctat tgccagcgga aactctagcc gattcaccgg
      961 gttttgggtg tggcttaatt aactcccagt gagctttatt tttgactgtt gcgcaatcgc
     1021 gttatgctag gattgtagcg ggcgtcgaag tccgcgcgat tgaccccaaa atgtcaccag
     1081 attatacctt ttccggcgca tgctggtata ctactcggcc acgcggtcgg gcgcggcccg
     1141 ccgggatttg acgcaccagg agacaactag atggccgata ttcaggcaac tgccaatcgc
     1201 atcgtcgaga acgtcgagcg ggtcatagtc ggcaagcggc agccggttca actgacggtt
     1261 ctggggctgc tctgccaggg ccacatcctc attgaggacg tgcccggcgt gggcaagacc
     1321 gtgttggcca agtcgctggc caagtcggtc ggctgcaagt tccagcgcat ccagttcacg
     1381 cccgacatgc tgcccagcga tgtgaccggc gtgtccgtct tcaaccaggg cacgcgcgag
     1441 ttcgagtttc gtcccggccc catccacgct cagatcgtcc tcgtggatga aattaaccgg
     1501 gccacgccca aaacgcagtc agccctgctg gaagcgatgg aggagcggca ggtgacggtg
     1561 gacggccgca cctatcctct cgacgtgcct ttcatggtcc tggccacgca gaaccccatc
     1621 gagtacgaag gcactttccc cttgccggaa gcccagctcg accgcttcat gttgcgcatc
     1681 cgtctgggct atcccggcaa ggaagaagag atcgagatcc tggatcggca gcagcacgcc
     1741 cacccgatca atgcgctgca acaggtcgtc gatgtggacg aactgctggc ggcgcagcaa
     1801 gcggtcaagg acatctacgt cgatccactg gtgaaggagt atatcgtcaa cctggtgcgc
     1861 cagacgcgcg accaccccga tgtctacctc ggctccagct cgcgcggggc gctggccatc
     1921 taccggctgg ggcaggcgcg cgcggcgctg atgggccgcg actatgtgct gcccgacgac
     1981 gttaaggccc tggccggccc ggcgctgggc catcgcatca tcgtcggccc ggcggcgcgc
     2041 atcaaggaca ccgagccgga gcagatcgtc caggacctgc tggataacat ggccgtcccc
     2101 ggcgcgcacg tcgggcggca acggacgcgc gtctaatgtc tggcttcctg gggcagcggc
     2161 gcacggtcgt cttcgccctg gccgtcgtgg ccttcatcgc tggcctgatc actggccgcg
     2221 atctgctgtt cacgttggcc tatctgctgg ggctgctgct gatcctgtcc ttcctctggg
     2281 cctgggtcaa ccttaacggc gttcacctct cgcgggtgac gcgcgtgcgc cggacgcaag
     2341 tgggccgacc gctggaagag cgcttcagcg ttcgcaacac cactgtcgtg cccaagctgt
     2401 ggttggaagt gcgcgactat tccacgctgc cctatcatta ctccagccac gtcgtcaatg
     2461 ggctgggcgg gcggcagaac tacagctggc gcgtgaccac catctgtcag cagcgcggtc
     2521 gttaccagct cggccccatc cgcctgcgca ccagcgaccc cttcggcctc ttcccgctgg
     2581 agcgcgagct gacaccgacc agcaacgtcg tcgtcttccc catgaccttc cccatccacc
     2641 agtttgcctt gccgttgggc gtcttgcccg gcggcgacgc gctgcgccgg cgcacccact
     2701 acatcaccac caacgccgcc ggcgtgcgcg actatgagcc gggcgacagc ttcaaccgca
     2761 tccactggcg cagcacggcg cggcgcgacc ggttggtggt caaggagttc gagctagacc
     2821 ccctggctga catctggatc gtgcccgaca tgtccgtcta tggccacgtg acgccgcgca
     2881 aagacgccgt gccgcccccg ccgccgggcg acattcccgg ttggttgaag atgcaggagt
     2941 tccaattgcc ggaatcgacc gaggagtacg ccgtgtccat cgccgcgtcg gtggcccaat
     3001 acttcctgcg gcgcgatcgc gcggtgggca tgctggccta cgggcagtcc aacgaaatcc
     3061 tccagcccga ccgcggcgag cgccagttca accgcattct agagacgctg gccgtgctgc
     3121 gggccgaggg cgatgtgccg ctgcaagaca tgctccacac cgaaagccat cttttcccgc
     3181 gcggcacgac gatcatcgct gtcacgccca tcacccgcga ggagtggtcg gtggcggcgc
     3241 ggcaactggc gcgccgcggg ttgcgcgtgg tgaccatcct ggtcaacccg gcctcgttcc
     3301 acggctcacg ctcggccgcg ccgctctatc aactcttgcg tgggggtggc caggtggcct
     3361 atatggtcaa caatggcgac gacctgacca cggccctcag cagcggctcc acccaggcgg
     3421 gcttcttcac cgtctagcgt cggggccagc catcggtaga atcacctcgg ccgtcgatgg
     3481 ccgcccctac atcgccagcg tcagagggat ggactcgccc gccggccggt gggccggcgt
     3541 ctgtggccca tcgtccacct tcttccccga catactccac ggccccgtgt gggtcacgtg
     3601 gacggccaca agttggccgc gcaggtcgcc cgcgtcgtcg aagaaaacga gcttgttgtg
     3661 gggcgtgcgg ccgcgccagc gccctttgtc gcgctcctca accagcacct cgaccgtctg
     3721 ccccaggtag cggcgcatct tctccgtcga aacctctttc tgcagcgctt cgatcatgtg
     3781 gaagcggcgg cgcttttcct cgtcgggcac gtcgtcggcc atgcgccggg cgctgaccgt
     3841 gcccggccgc gggctatagc gggccaggtg cagcttgtcc agccgcaact cggccaggat
     3901 gtcgtaggtg gccatgaact gcgcctcgct ctcgccgggg aagccgacga tcacgtcgca
     3961 gtggatggcc gcgtcgggga tgaccgcgcg gacgtgctgc accaattcgc ggtattgctc
     4021 gcgcgtatag ccgcgcttca tcgcttccag cacgtcgttg tcgccggcct ggatgggcac
     4081 ttcgatctgg ggcatcacct tgggcagctc ggccacggcg cgcaggatgc ggtcggtcat
     4141 gtagttgggg tggctagtca ggaagcggat gcgctccagt ccgtccacgt cgttgaccac
     4201 gcgcaatagg tcggccaggt cggggccgtc ggggatgtcc gtgccgtagc gatcgacgat
     4261 ctgccccagc aggacgacct ctttgacccc ccgcgcggcc agcccgcgcg cctcggcaac
     4321 gatctcgccc actggccggc tgcgttcgcg gccgcgcttc tgggggatga tgcagaaggt
     4381 gcaggcgtgg gagcagccgt agacgacggc cacgggggcg gagatgggga gcgggggagc
     4441 gggagagcga gggggcaggg gggcaagggt gatcttcccc gcatcttcac catccatcag
     4501 cgcatgtcgc gccgccgttt ccgccaactc cagggcatag tcgctatcct gagtcaggtg
     4561 gctaaccagc ggcagaccgt cggtcgatgg ctccatgaag acatcgacaa agggaaagcg
     4621 ctgggtcagg gcctcgttgc cgcgcacacc aacgacgcag cccatcacgg ccacggtcat
     4681 gtccggccgg cgcgctttca gcgggcgcag gctttgcagc ttgtggtagg ccttgtcttc
     4741 cgactgttgc cggacggtgc acgtctccag cacaaccacg tcggcatcct cagcgcgggg
     4801 cgtgggccga tagccgagct tctccagttc ggtggccact cgccgcgcgt cggcgtagtt
     4861 catctgacag ccggtgatcc agaagtgata ggtcttggtc atcgcttacc cgtcgagcat
     4921 tgggattcta gtgatcggcg cgcgtcaagt caagcggcct actcatccag ccaggccacc
     4981 gacgtacacg cgccggcgtt gtggcgcagc agccgcacct ggccggtgtc ggggatgacc
     5041 aggcccaaca gattatcatc cagggcgaca atcagccggc cgtcccgcgt ccaatcataa
     5101 gtgaccgacg gcaggaagta gggcaaccgg gacagcaagg gaatcgtgcg gttggcggcg
     5161 atgtcgtgca gtagcatggg cgcgaggttg ctgccgcccg tgtagctcat ggagccgacg
     5221 cccgtcagca ccagatagcg gccgtcgggc gagaagccca gtgattggtt gaagtccgcc
     5281 cccagggtca ggcgcagttc gatctgcccc gtcgcccggt cgaaaaggaa gacgtaggcg
     5341 tcttcggcca cttcgtcccg cgcgacgata aacaggctat ctggctggct cgggttgggg
     5401 gcgacatagg ccaggttcat ctgccgcatc cggctgtcgt ggggcaggaa ctggaacaga
     5461 tcggcgctca gcaacagcgt ctgcggcgta gagtcatcga ccgtggccac gacgatctcc
     5521 tcgccgccgt cggtcgtgga tgtcgcgccg cccacggggc ggatgaagcc gaaagtgcgc
     5581 tcatcgatcc agaaaggcgc atagccctga ccgaggatga caggtcggcc tgagtcgccc
     5641 gcggtcccca gggcgatggg catgacggca aacggttcat tcgagtcgag aagcaggtag
     5701 cgaccgttga cgaccagttg tgggccagga ttctcgctgc cgtcgccctg gtagagcgcc
     5761 cactgaccat tgggcgacca caggggctgg cccggcgagg cctccgtggc gcaggtgttg
     5821 ccctcgcaag ccgtcaggtc agtgaccagc gcgcggatgt cgttgcccag cgtatcgcgc
     5881 tggtagatca cgagacgtgt cccggccgcg tccgactggc ccagcgaaat ggctgaactg
     5941 gggtcgtctg tcgtgtaggc ggtggcaatc tcgccggcgc gccagtagcg gctctgccag
     6001 gtatcggtgg ccacggtgta ttgttgcagc agcatggttt cccagcctgg cagcggggtc
     6061 atccagatga agccctgcac gctgtagaga tgctcccagt caccagtggc tggcaagtag
     6121 cgcaacaact gggatgtctc cggcgtctgc gcctcgcccg gggcggtgca gaccaggtag
     6181 agcgtttcgt cgggtaaggg cgcttcgctc tccgcttcgg cggcgcgctg gatggcgtag
     6241 agccagaggg cctgggtcag gtcggcgggc acaggccggc cggccgcgcc gtcttgtagc
     6301 acctgctggg cgaactggtt gaaactgcgc acgctgctca accgccgttg caggtcggcc
     6361 ggcgagaggt cgccggcatg tagcagaaaa tcgacggcca cgcgcgccag ccacagccga
     6421 tccggatcga tctctgccgg cagccgctcg ttggtcaaca ggttgagccg ctgcatgggg
     6481 atgcgctcat ccagcacgcg gcggtagtcg tccgggccga ccggcggcgc ggatacgagg
     6541 ccaagatcga ccagctggta tggcagcagc atcgagaaca gcagcgacgc atcgcagcag
     6601 cgccacgaca ctgcctgggc aatggccgcg ccgaggacgt ggcgggcgta gccgtcgcgc
     6661 agggcggcgt aggcggcctt gcgctcgccc tcgtcgcccg ccggcagccc gaccagcgac
     6721 ggcgccggca actccaggat gttggcggtc tggcgcgctc gccgcagcgc gccgatgggc
     6781 tgtgacagat cggccagcgt ctgtggctct gtgtccaggc ggacggtcag gaagaggtcg
     6841 gccgaacagt cgatgtccgc cagcgtggcg cacatgccgg cgatggcggc gtcgaggtcg
     6901 atggccaact gctcggcgat gacggcgtcg cgctcggggt agatgagatt caggtagtcg
     6961 ccctcgctcg attgccactc gccccagaag tcgcccaccg gcggggccag tagccagcgc
     7021 cggtcgccgc gccggaagac ggtcgtctgt tgcaacaggt cggtgccgcc gtcgtcgcgg
     7081 cggtagggct ggtcgacagt gacgatagct tcgtt
//
LOCUS       DAOSMQ010000110         7107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10588_length_7107_cov_4.995604, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000110 DAOSMQ010000000
VERSION     DAOSMQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10588_length_7107_cov_4.995604"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PK829_05185"
     CDS             complement(<1..1347)
                     /locus_tag="PK829_05185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU40641.1"
                     /translation="MTHHRHRALAVGLLVLYFAAAVHMAFALPALATPNELLNFEYVQ
                     VMRQIGGLPNRGLVDSEVRYTEWHQPPLYFAFAALFGLAVPVPPSADNPPPPIAMPAN
                     PHYLSTPAGNRNPVVHVNPANTPLLYTSRVAAALLGALGVAALHAAGKRVYGPAAGLL
                     MASLLAFQPTYIHLSGSVNNDMPLTAVVAIVMSYAVLLVERSSRQGREGSKGRKEEIS
                     RKDAENAKLRTLSFAFSASLREIFPFFVLGLLCAAAILTKANGVFVLVFPAVVLLGVL
                     VGGRRRTTNDRRPPTADRQPRPTGPLPTAHWLPAAAATAAGLLPLWVGWLWLNAVRMH
                     DALGLSGSLPVGRVLRLGPLDFNHVIPFLPAIWRSYWLDWSAGDVGYGPDWLYALWLM
                     AVVVMLLGWLRPMRLIRNYKLGIRNREESSRKGRQEEISSWRPWLPWRPLRELFLAH"
     gene            1517..4018
                     /locus_tag="PK829_05190"
     CDS             1517..4018
                     /locus_tag="PK829_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOU40642.1"
                     /translation="MIDTKGKYYLGKQWDAGASQLLDDTLLYDSEDLTTHAVVVGMTG
                     SGKTGLCVGLLEEAALNGVPALMIDPKGDLTNLLLHFPDLLPADFKPWINTDAARKDG
                     KTEDQAAAETAEMWRNGLADWDIAPERLQALAGSVRFTIYTPGSTSGVPLSVLASLKA
                     PDLPWQGNEEVLQEQISGTVTALLSLTGLRDIDPVQSREHILLSNIVANAWSQAHDLD
                     LGELIMQIQSPPFTKLGVFDVNTFFPEKDRTGLAMRLNSILAAPSFQTWVQGEPLDVG
                     ALLYTPDGRPRHSIFYIAHLSDDERMFFVTLLYANIESWMRAQSGTTSLRALVYFDEI
                     FGYLPPIGNPPSKTLMLRMLKQARAFGVGLVLATQNPVDVDYKGLSNIGTWFIGKLAT
                     DQDKARLLDGLSSADGSVDVRELNRLISGIGKRVFLMRNVHEKKPLLFQTRWAMNYLA
                     GPLTRTQIPALNALKGAGPIAAGPAGAAAAATMAAPPPAEAPSRPATPGTPDAEGTVT
                     RPPVPTGVGEYFLPANLSLAEAAARERVSLPAGARESGILYRPALLAQAEIALNNAKY
                     GLNTRTTWAALATDTADLRWEDVAVAPLDDRGLDRAPIRDARFAALLAPLSDAKAMRA
                     LETNLLDYIVRDVGVTVRANDRLKVYAGPDVDEAAFEEMCRDAADDQMQAELDKVQAR
                     FAARLKTAEERLKREERELAEDQADYEGRKREEYAKHAETLLGFLGGRRKSLSSSLSK
                     RRMTEKAKADIEESEQAIADLQEQVGDLKEEMEAGLDEVEAKWQALLGDTKEVTVAPR
                     KTDVRLPLFGVAWLPTYQVVDANGRVVPLPAYGAP"
     gene            complement(4099..5406)
                     /locus_tag="PK829_05195"
     CDS             complement(4099..5406)
                     /locus_tag="PK829_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOU40643.1"
                     /translation="MTTPAGSKRRQVATALLPLLLALLAACSGPPPLLDVPQPGPPVA
                     ATRGASTPAAGRTPLPTVAPVAPTPAVSPPAPPVAPSPPVTAPAPLPTLPAAGGQLPR
                     DIGYDAGAWAVLFSPGARGNQANGQYIIDKLIGYIDAAQSTIDLAVFETNLTPVAEAL
                     IRAHARGVAVRWITDDENGIDADDEAGRGQFALLEAAGIPVRDDLRSAFMHNKFILID
                     GRILWTGSMNLTTNDVFRNNNNVVVLDSPAAVAIYEREFEEMWSGQFGPGSPSNVNGQ
                     TVQTAAGLPITIRFAPEDEPFDSLVQLVALANESIRFMAFSFTEDELGAVMRARASEG
                     VVVQGIFETRGSETAYSEMPAMFCAGLEVRQDGNPGTFHHKVIIIDDFVVVTGSMNFS
                     DNAADSNDENVIAIADPAIGRLYREEFVRRWAEATTPDIACEN"
     gene            complement(5384..5983)
                     /locus_tag="PK829_05200"
     CDS             complement(5384..5983)
                     /locus_tag="PK829_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40644.1"
                     /translation="MSDSPPDDLPEAPDPAELPAAAQWMMAQAAAANTPAERAESELR
                     FTSLPEREARIVGGLRANHKLTAGLEPGAAAALTEWGAAMGRFVVAETAGLDDATAEG
                     ILQVRVRAARQLMVHVARAITDPPPPGVAPLDTALRQADIVYGRRYRAPDGNAVGRAL
                     AHWSAAAGQPARQIAGLRSFVEEHIRPQTGAAGDDASRV"
     gene            complement(6120..>7107)
                     /locus_tag="PK829_05205"
     CDS             complement(6120..>7107)
                     /locus_tag="PK829_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40645.1"
                     /translation="PTYDALAGERVWSFSFVPDDGVYPYHSTESFGPVKMGCVIQFVQ
                     IDDDVDERINSFSINADKVYTVAQGMVTYGSFIVPRDGELLFEAVDSVGLVIRVCQDK
                     VTLTPSVTPTETPTASLTPTITPTFTPTLTLTPTLTLTPTLTLTPSATPTEAMTATPT
                     ATGTQPPTETPTATATTPITPTVPTNTPTVTMTPTETPTASPTLTPPPGPNEMTATPT
                     ATAEPDIPRLPACLRIDFEVGPDSARRGTYVVQEVGGHVLATWWAEEGWMDSGWVRDL
                     DITFPAVYVQVFWVKGDGSPPVELTILNPAPDTNYGWLARGRCHALEVAWPG"
BASE COUNT         1204 a         2390 c         2414 g         1099 t
ORIGIN      
        1 atgagcgagg aagagctcac gcaaaggacg ccaaggaagc caaggacgcc aagaagaaat
       61 ctcttcttgg cgtcctttgc gtgagctctc ctctcgattc ctaattccta atttgtaatt
      121 cctaattagc cgcatgggcc gcagccagcc cagcagcatg accactaccg ccatcaacca
      181 gagcgcgtag agccagtccg gcccatagcc cacgtcgccg gccgaccagt cgagccagta
      241 actgcgccag atggccggca ggaaggggat cacatggttg aagtcgagcg ggccgaggcg
      301 caggacgcgg ccgacgggca ggctgcccga cagccccagc gcgtcgtgca tgcgcaccgc
      361 gttgagccac agccagccca cccatagcgg cagcagaccg gcggctgtgg cagcagcggc
      421 agggagccag tgggcagtgg gcagtgggcc agtggggcgc ggttggcggt cggcagtcgg
      481 cggtcggcgg tcgttcgttg tccgtcgtcg gccgccgacc aacaccccca gcaacaccac
      541 cgccggaaac accagcacaa acactccgtt ggccttcgtc aaaatcgctg cggcgcacag
      601 tagtcccagg acgaagaagg ggaagatctc acgcaaagac gcagagaacg caaagctaag
      661 agttcttaac tttgcgttct ctgcgtcttt gcgtgagatt tcttctttgc gccctttgct
      721 cccttcgcgt ccttggcgtg agctcctctc cactaacaac acagcgtaac tcatgacgat
      781 ggccaccacg gctgtcaggg gcatatcgtt gttgaccgag ccgctcaggt gaatgtaggt
      841 cggctggaag gccaggagcg aggccatgag caggccagcg gccgggccat agacgcgctt
      901 gccggcggca tggagcgcgg ccacgcccag cgcccccagc agcgccgcgg ccacccggct
      961 ggtgtagagc agcggcgtgt tggccgggtt gacgtggacg accgggttgc ggttgccggc
     1021 cggcgtggac aggtagtggg ggttggccgg catcgcgatg ggcggcggcg ggttgtcggc
     1081 cgacggcggc acgggcacag ccagcccgaa gagcgccgcg aaggcgaaat acagcggcgg
     1141 ctgatgccac tcggtgtagc gcacttcgct atcgaccagg ccgcggttgg gcaggccgcc
     1201 gatctggcgc atgacctgga cgtactcgaa gttgagcagt tcgttgggcg tggccagggc
     1261 tggcagggcg aaggccatgt ggacggcggc ggcgaagtat aggacgagca gccccacggc
     1321 cagcgcccgg tggcgatgat gcgtcataca ggccgcatta tagcactacc actgccccgc
     1381 cagtatctcg gtgcagggca agcgcataga tgtggctgcc ctggggaaga acgctcttcc
     1441 ctagccccta atccctaacc cctttcttag cgttcttccc gccttgcgct acacttgggc
     1501 gtggaggtgc gtgagcatga tcgacaccaa aggcaagtac tacctgggca agcaatggga
     1561 cgccggggcc agccagctcc tcgacgacac cctgctctac gattccgaag acctgactac
     1621 ccacgccgtc gtcgtcggca tgaccggcag cggcaagact ggcctgtgcg ttggcctgct
     1681 ggaagaggcc gccctcaacg gcgtgcccgc gctgatgatc gaccccaagg gcgacctgac
     1741 caacctgttg ctccacttcc ccgacctgct gccggccgac ttcaagccgt ggatcaacac
     1801 cgacgctgcc cgcaaggacg gtaagacgga agaccaggcc gccgccgaga cggccgagat
     1861 gtggcgcaac ggcctggccg actgggacat cgccccggag cgcttgcagg cgctggccgg
     1921 cagcgtgcgc ttcaccatct acacgcccgg ctccacgtcc ggtgtgcccc tcagcgtgct
     1981 ggcctccctg aaagcgcccg acttgccctg gcagggcaac gaggaagtgc tgcaagagca
     2041 gatcagcggc acggtgaccg cccttctgag cctgacgggc ctgcgcgaca tcgacccggt
     2101 gcagagccgc gagcacatct tgctgtccaa catcgtcgcc aacgcctgga gccaggccca
     2161 cgacctcgac ctgggcgagc tgatcatgca aatccagtcg ccgcccttca ccaagctggg
     2221 cgtgttcgac gtcaacacct tcttcccgga gaaagaccgc accggcctgg ccatgcgcct
     2281 gaacagtatc ctggccgcgc ccagcttcca gacctgggtg cagggcgagc cgctggacgt
     2341 gggcgcgctg ctctatacgc ccgacggccg gccgcgccac tccatcttct acatcgccca
     2401 cctgagcgac gacgagcgca tgttcttcgt gacgctgctc tacgccaaca tcgagtcgtg
     2461 gatgcgcgcc cagagcggca cgacctcgct acgggcgctg gtctacttcg acgagatctt
     2521 cggctacctg ccgcccatcg gcaacccgcc ttccaagacg ctgatgctgc ggatgctgaa
     2581 gcaggcccgc gccttcggcg tcggcctggt gctggccacg cagaacccgg tcgatgtcga
     2641 ttacaagggc ctgtccaaca tcggcacgtg gttcatcggc aagctggcca ccgaccagga
     2701 caaggcccgg ctgctcgacg gcctatccag cgccgatggc agcgtcgatg tgcgcgagtt
     2761 gaaccggctc atctccggca tcggcaagcg cgtcttcctg atgcgcaacg tccacgagaa
     2821 gaagccgctg ctcttccaga cgcgctgggc gatgaactac ctggccgggc cgttgacgcg
     2881 gacgcagatc ccggccctca acgccctcaa gggggccggc cccattgccg ccggcccggc
     2941 cggagccgcc gccgcggcaa caatggccgc gccgccgccg gccgaagcgc cctcgcggcc
     3001 ggccacgccg ggcacgcccg acgccgaggg caccgtcacc cgtccgcccg tgcccaccgg
     3061 cgtgggcgag tacttcctgc ccgctaacct gtcgctggcc gaagccgccg cgcgggaacg
     3121 ggtcagcctg cccgccggcg cgcgcgagtc aggcatcctc taccggccgg cgctgctggc
     3181 ccaggcggag atcgctctca acaacgccaa atacggcctg aacacgcgca cgacgtgggc
     3241 ggccctggcc accgacacgg ccgatctgcg ctgggaagat gtcgccgtgg ccccgctgga
     3301 cgaccgcggc ctcgaccgcg cccccatccg cgacgcccgc tttgccgccc tgctcgcccc
     3361 cctgtccgac gccaaggcca tgcgcgccct ggagaccaac ctgctcgact acatcgtgcg
     3421 tgatgtcggc gtcacggtca gggccaatga caggctgaag gtctacgccg ggccagacgt
     3481 ggacgaggcc gcctttgagg agatgtgccg cgacgccgcc gacgaccaga tgcaggccga
     3541 actggataag gtccaggccc gcttcgccgc ccggctgaag acggccgagg agcggctgaa
     3601 gcgcgaggag cgcgagctgg ccgaagacca ggccgactac gaagggcgca agcgcgagga
     3661 gtacgccaag cacgccgaga cgctgctggg ctttctgggc gggcggcgca agagcctgtc
     3721 cagctccctt tccaaacggc gcatgaccga gaaggccaag gccgacatcg aggaatcgga
     3781 gcaggccatt gccgacttgc aggaacaagt gggcgatctg aaagaggaga tggaggccgg
     3841 gctggacgaa gtcgaggcca agtggcaggc gctgctgggc gacacgaagg aagttaccgt
     3901 cgcgccgcgc aagaccgacg tgcgtctgcc gctgttcggc gtagcctggc tgcctaccta
     3961 tcaggtcgtg gacgccaacg ggcgggtggt gccgctgccc gcctatgggg cgccgtaaga
     4021 taggggctag gggttaggtt ctaggggcta gggaagagcg ctcttcccta gcccctagtc
     4081 cctagcccct atcttcccct agttctcgca agcgatgtcc ggcgttgtgg cctcggccca
     4141 gcgccggacg aactcctcgc ggtagaggcg gccgatggcc gggtcggcga tggcgatgac
     4201 gttctcgtcg ttgctgtcgg cggcgttgtc agagaaattc atcgagccag tgacgaccac
     4261 gaagtcatca atgatgatga ccttgtggtg gaacgtgccc gggttgccgt cctgccgcac
     4321 ctccagcccc gcgcagaaca tagccggcat ctcgctgtaa gccgtctcac tgccgcgcgt
     4381 ctcgaagata ccctgcacga cgacaccctc gctggcccgc gcgcgcatca ccgcgcccaa
     4441 ctcatcctcc gtgaaggaga aggccatgaa gcggatgctt tcgttggcca acgccacgag
     4501 ctgcaccaga ctgtcgaacg gctcgtcctc cggcgcgaag cggatggtga tgggcagccc
     4561 cgcggccgtc tgcaccgtct gtccgttaac gttggacggc gaccccggcc cgaactgccc
     4621 cgaccacatc tcctcgaact cgcgctcgta gatggccacc gccgcgggcg aatcgagcac
     4681 gacgacgttg ttgttattgc gaaagacgtc gttggtcgtc aggttcatcg accccgtcca
     4741 gaggatgcgg ccatcgatga ggatgaattt gttgtgcatg aaggccgagc gcaagtcgtc
     4801 gcgcacgggg atgccggcag cctccagcag ggcgaactga ccgcgacccg cctcgtcgtc
     4861 ggcgtcgatg ccgttctcgt cgtcggtgat ccagcgcacg gccacgccac gcgcgtgggc
     4921 gcgaatgagc gcctcggcca ccggcgtcag gtttgtctcg aagacggcca ggtcgatggt
     4981 gctttgcgcg gcgtcgatat agccgatgag cttgtcgatg atgtactggc cgttggcctg
     5041 gttgccgcgc gcgccggggc tgaacagcac ggcccacgcg ccggcgtcgt agccgatgtc
     5101 gcggggcagt tggccaccgg cggccggcag cgtcggcagg ggcgcgggag cagtgacggg
     5161 cggcgacggg gccacgggcg gcgcgggtgg cgacacggcc ggcgtcggcg cgacaggggc
     5221 aacggtgggc aagggcgtgc gcccggccgc cggtgtagac gcgccgcgcg tggcggcgac
     5281 cggtggccct ggctggggca cgtcgagcaa gggcggcggc ccgctgcacg ccgccaacag
     5341 cgccaataac aacggcagca gcgccgtcgc gacctggcgg cgcttagacc cggctggcgt
     5401 cgtcaccggc ggcccccgtc tgtggacgaa tatgctcctc cacgaacgac cgcagcccgg
     5461 cgatctggcg cgctggctgc ccggcggcgg ccgaccaatg agccagcgcc cggcccacgg
     5521 cgttgccgtc cggggcgcgg tagcgacggc catagacgat gtcggcctgg cgcagggccg
     5581 tgtctagtgg cgcgacaccg ggcgggggtg ggtcggttat ggcgcgggcg acgtggacca
     5641 tcagttggcg ggcggcgcgc acgcgaacct gcaagatgcc ctcggccgtg gcgtcgtcca
     5701 ggccagccgt ttcggcgacc acgaagcgcc ccatggccgc gccccactcg gtcagcgcgg
     5761 cggcagcgcc cggctctagc ccggcagtca gcttgtggtt ggcccgcagg ccaccgacga
     5821 tgcgcgcctc gcgctcgggc agcgacgtga agcgcagttc ggactcggcg cgctcggccg
     5881 gggtgttggc cgcggcggcc tgggccatca tccattgcgc cgcggcaggc agctcagccg
     5941 ggtcgggcgc ttcgggcaag tcatcgggcg ggctatcgga catacgctcc tcgctcacga
     6001 acggatggtc tagctgcgcc tcattgtacg ccaacgcggc cagccggcca aaagtaaagg
     6061 acgccaggct ccgcagacag agcctggcgt cccattccaa tacgatgtcg ttgtttggtc
     6121 tagccgggcc aggccacttc cagggcatgg cagcgcccgc gggccagcca gccatagttc
     6181 gtgtccgggg ccggattgag gatcgtcagt tctactggcg gcgagccgtc gcccttcacc
     6241 cagaagacct gcacatagac ggccgggaag gtgatgtcca aatcgcgcac ccagccgctg
     6301 tccatccagc cctcttcggc ccaccacgtc gccagaacat ggccgcccac ttcctggacg
     6361 acataggtgc cgcgccgggc gctgtccggg ccgacctcaa agtcaatgcg cagacaggcg
     6421 ggcagacgcg ggatgtccgg ttcggccgtc gccgtcggtg tggcggtcat ctcgttgggg
     6481 ccgggcggcg gcgtcagcgt cggcgaggcc gtgggcgtct ccgtcggcgt catggtgacc
     6541 gtcggcgtgt tggtgggcac ggtcggggtg atgggcgtgg tcgccgtggc cgtcggcgtc
     6601 tccgtcggcg gttgcgtgcc tgtcgccgtt ggcgtggctg tcatcgcctc ggtcggcgtg
     6661 gccgaggggg tcagcgtcag cgtcggcgtc agcgtcagcg tcggtgtcag cgtcagggtt
     6721 ggcgtgaacg tcggcgtaat cgtcggcgtc aggctggccg tgggcgtctc cgtcggcgtc
     6781 accgaggggg ttagcgtgac cttgtcctgg caaacgcgaa tgaccagacc aacgctgtca
     6841 accgcttcaa acagcagttc accatcgcgc ggaacgatga atgagccata cgtcaccata
     6901 ccctgggcga cagtatagac cttgtcggcg ttgatgctga agctgttgat gcgctcgtcc
     6961 acgtcgtcgt cgatctgcac aaactggatc acgcagccca tcttcaccgg gccgaagctc
     7021 tcggttgagt ggtaggggta cacgccgtca tcgggaacga agctgaagct ccagacgcgc
     7081 tcgccggcca gggcatcata cgtcggg
//
LOCUS       DAOSMQ010000111         7099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10608_length_7099_cov_4.926320, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000111 DAOSMQ010000000
VERSION     DAOSMQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10608_length_7099_cov_4.926320"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PK829_05210"
     CDS             <1..568
                     /locus_tag="PK829_05210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOU40646.1"
                     /translation="NLPAGGRLLDIATGTGDIADEGRRQVPGLRAVGGDFTIEMMRAG
                     KRLPGRETIHWVAADTLALPFPDETFDAVTSGFLMRNVIDVAGAFREQWRVTRPGGRV
                     VVLESSPPKDNLLRPFIKLHLNKVIPTLGRLITGESDAYRYLPDSTQGFQGPAELAAT
                     MRAGGFVDVSYQLYMFGTIAIHAGTKPR"
     gene            637..1473
                     /locus_tag="PK829_05215"
     CDS             637..1473
                     /locus_tag="PK829_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_256440082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1012..1014,aa:Sec)
                     /transl_table=11
                     /product="Rcas_1661 family thioredoxin-like
                     (seleno)lipoprotein"
                     /protein_id="HOU40647.1"
                     /translation="MRRFLLGLLLLLAACAPAAGETGAPASSPVVMTATTAPTATTAP
                     AATTVRESAAATATTVVTATAPAGGEQGSAAATADSPAAATGEPEPTAAPSSPIAGRN
                     DDGTFFYGAAAAPVTLTDYSDFLUSTCRAHVLGAERDIVANHVTAGEVRLVFWPILDL
                     GPNSLNAATTAFCAGEQDPALFWAAHDLLFANQGEVYGADRDYFLNVAAGLGLDADAF
                     AACYDGDDVRAQLVDLDATRRQQGINQRPTFALVSDAGTRRLIGAQPYDTFAAAITAL
                     LP"
     gene            1642..2268
                     /locus_tag="PK829_05220"
     CDS             1642..2268
                     /locus_tag="PK829_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU40648.1"
                     /translation="MSTLDLSGQVAIVTGAGQPAGAAVARALAAAGARVAVNDLNPDR
                     IARLAAELRAAGGQALDIAADTASKFQCVTLIEATRAEWQRVDILVNATSVRPAAAVL
                     KMDEWEWARCHDVNLKAAFFMCQLVGRVMDDQNRAEPRARGGLIVNVAWPAPDEDGRA
                     AYAAGQAGLVAFGRVCAREFAPLGVRVATVMAAPAAPEAVAADVLALL"
     gene            2280..3602
                     /gene="mtaB"
                     /locus_tag="PK829_05225"
     CDS             2280..3602
                     /gene="mtaB"
                     /locus_tag="PK829_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOU40649.1"
                     /translation="MKIHLHAIGCRLNQAEIETMARQLAAGGHTPTADPAEADVIILN
                     TCAVTAAAARDARNLTRRFARANASAEIVLTGCYATLAPAELAALPGVARVVDNASKA
                     SIPLLLDPALPTDAPLFDREPLARETAAGSIGRTRGFIKVQDGCDNRCTFCVTTIARG
                     EGRSRPLAEVVAEVGACAAAGYQEVVLSGVHLGSYGRDQGRPAGLRELVAALLADTDI
                     PRIRLSSLEPWDVAPGFFDLWANPRLLPHLHLPLQSGSDRVLRRMARRTTRAAFRALT
                     DEARAAIPDLNLSTDLITGFPGETDADHAATLAYVEALGFGRLHVFSYSPRPGTAAAR
                     MPDQVSGPVKKSRAAALIALGERLGAAFHTGYDGATRPVLWEMATGAEANGLRWVGYT
                     DNYIRVTATGPTGLMNRVTLARLSRPRADGMAGEIDPASASNSDSGEE"
     gene            3599..4171
                     /locus_tag="PK829_05230"
     CDS             3599..4171
                     /locus_tag="PK829_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOU40650.1"
                     /translation="MNRMDNQKELSPEQRAQLLKTLQARFEKHMNRHNGLEWAAVQAR
                     LNANPGKLWSLSEMERTGGEPDVVGQDEQTGAYIFYDCAAESPKNRRSLCYDPEALWS
                     RKENKPADSAVGMASDMGIELLTEGQYRALQELERFDTKTSSWLKTPANIRQLGGAIF
                     ADYRYGHVFVYHNGAESYYAARGFRGSLKV"
     gene            complement(4168..5022)
                     /locus_tag="PK829_05235"
     CDS             complement(4168..5022)
                     /locus_tag="PK829_05235"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOU40651.1"
                     /translation="MSKRLLITGGSGYLGRHLARRVAAGWPGEWRYTIFSADPLRLPQ
                     STRLDLRDAAAVARVVADFAPDAIIHTAGSNRTPDMSAVIVAGTAHIAAAATQAGARL
                     VHLSTDALFGGDAAPYDESAPPAPRNDYGRAKAEAEALALAHANAVAVRTSLIYGLEE
                     MDNGTAWMAAALRAGQPVTLFTNQRRNPVWVETLAAACLELVDQAYTGVLNVAGRQTL
                     TRAAFALRLLDWWGVGERATLTLAEDASGRWPLDCALDVSLAGRLLRTPLPGVDEVLR
                     AKARGRSG"
     gene            complement(5150..5851)
                     /gene="rnc"
                     /locus_tag="PK829_05240"
     CDS             complement(5150..5851)
                     /gene="rnc"
                     /locus_tag="PK829_05240"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU40652.1"
                     /translation="MDDLLTFSEQLGVEFRDVSLLSRALTHRSYLNENPGSVLEDNER
                     LEFLGDAVIDFVVAGFLYHHFPEMDEGELTALRAALVRAESLAAFARQIDLGRYLRLG
                     LGEEENGGRERTPLLCATFEAVVGAIYLDHGLERARAFIDPLVRPALEHIRAGELYKD
                     AKSEFQVWAQARYNITPHYRVSGSEGPDHARTFTVQVLVGPEVWGEGRGRSKQIAAQA
                     AAAEAMRRAETTPEE"
     gene            complement(5940..>7099)
                     /gene="fabF"
                     /locus_tag="PK829_05245"
     CDS             complement(5940..>7099)
                     /gene="fabF"
                     /locus_tag="PK829_05245"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU40653.1"
                     /translation="AILAGRSGFGPITLVDNPQHIIQGLCEVKEFDPAQYMDRKDARR
                     RDRYQQLATAAAAEAMRGAGLAITDDNRERVGITLGTGIGGMRTIIEQEAIVHDGGLR
                     RLSPFGITMIMPNGAAGMLAIDYGITGPSTTITTACAAGNDAVGHAFRSIRAGELDAV
                     LTGGSESILTSVAIGGFERAGATSTRATDTPRPFDADRDGLVIGEGAGILVLESLEHA
                     RARGATILAEIAGYGQATDAYHITAPPENGNGAARAIRKALADAGLRPEDVDYVSAHG
                     TATPLNDTAETAAVKSALGEHAYNVAISSTKSMTGHIMGATGAIESVFCALAIRDQIA
                     PPTIHYETPDPTCDLDYVPNAARPRRVRVCLNNAFGFGGHNAVLAIRAFEE"
BASE COUNT         1117 a         2533 c         2344 g         1105 t
ORIGIN      
        1 caacctgccg gccggcggcc gtctgctcga catcgccacc ggcacgggcg acatcgccga
       61 cgagggccgg cggcaagtgc ccggcctgcg cgccgtcggc ggcgacttca ccatcgagat
      121 gatgcgcgcc ggcaagcgcc tgcccggccg cgagacgatc cactgggtgg cggccgacac
      181 gctggcgctg cccttccctg acgagacctt cgacgccgtc acctccggct tcctgatgcg
      241 caacgtcatc gacgtggctg gcgcgttccg cgagcagtgg cgcgtgacgc gccccggcgg
      301 gcgcgtcgtc gtgctggaat ccagcccacc caaagacaat ctgctgcggc cgttcatcaa
      361 gctgcatctc aacaaggtca tccccacgct cggccggctc atcaccggcg agtccgatgc
      421 ctatcgctac ctgcccgaca gcactcaggg cttccagggg ccggcggaat tggcggcgac
      481 catgcgggcc ggcggttttg tggatgtaag ctaccagcta tacatgttcg gcactatcgc
      541 cattcacgcg ggcacgaagc cgcgctaaat agtcgtgatc gttcttaatc gacttgtaag
      601 aaccttttcg gccagtcgcc gcttatacta atgtgcatga gacgttttct gctaggtttg
      661 ttgttgttgc tggcggcctg cgcgccggcg gccggagaga caggcgcgcc ggcaagctcc
      721 ccggtggtca tgacggcaac cacggccccg acggcaacca cggccccggc ggcgaccaca
      781 gtgcgggaga gcgcagcggc gacggccaca accgtcgtta cggccacggc cccggcgggc
      841 ggcgaacagg ggagcgcagc ggcaacggcg gacagccccg ctgccgcgac cggggagcca
      901 gagccgacgg ccgcgccgag cagccccatt gccgggcgca acgacgatgg cacgttcttc
      961 tacggcgcgg ccgcggcccc ggtgacgctg accgactact ccgacttcct ctgatcgacc
     1021 tgtcgcgccc acgtgctggg cgcggagcgg gacatcgtcg ctaaccacgt gactgctggc
     1081 gaagtgcgcc tcgtcttctg gcctattctt gacctggggc cgaacagcct caacgcggct
     1141 acgacggcct tctgcgcggg cgaacaagac ccggccctgt tctgggccgc ccacgacttg
     1201 ctctttgcca accagggcga ggtctacggc gcggaccgcg actacttcct gaatgtcgcc
     1261 gccgggctgg ggctggacgc cgacgccttc gccgcttgct acgatggcga cgacgtgcgc
     1321 gcccaactgg tcgatctcga cgccacccgc cgccagcagg gcatcaacca gcggccgacg
     1381 ttcgcgctgg tctcggacgc gggcacgcgc cgcctcatcg gcgcgcaacc ctacgatacc
     1441 tttgccgccg ccatcacggc cctgttgccc tagcagcggg cagacatgcc ggtctgccct
     1501 tctctccgag gcgcgccgac ctgaccggtg ggcggccacg aatggtacgt ccacatgtca
     1561 ggtcaatgat cgctagatga ctttgggaaa gccctgtccg tgcatgcttg cgttcccttc
     1621 tccctccgct atcatctctc catgagcacc ctcgatctct ccggccaggt ggctattgtc
     1681 accggcgcgg gccagccggc cggcgcggcc gtggcccggg cgctggcggc ggccggcgcg
     1741 cgcgtggccg tcaacgacct caaccccgat cgcatcgccc ggctggcggc cgaattgcgc
     1801 gctgccggcg gccaggccct cgacatcgct gccgacacgg ccagcaaatt ccagtgcgtc
     1861 accctcatcg aggcgacccg cgccgagtgg cagcgcgtcg atatcctcgt caacgccacg
     1921 tccgtccgcc cggcggcggc ggtgctgaag atggacgagt gggagtgggc gcgctgccac
     1981 gacgtcaacc tgaaagcggc tttcttcatg tgccagctcg tcggccgggt gatggacgac
     2041 cagaaccggg ccgaaccccg cgcccgcggc gggctgatcg tcaacgtcgc ctggcccgcg
     2101 ccggacgagg acggtcgcgc cgcctatgcc gccgggcagg ccgggctggt ggctttcggc
     2161 cgcgtttgcg cgcgcgagtt cgcgccgctg ggcgtgcgtg tcgcgacggt tatggccgcc
     2221 ccagccgcgc ccgaggctgt cgccgcggat gtattggcgt tgctgtaggg tgaatagaga
     2281 tgaaaatcca ccttcacgcc attggctgcc gcctcaacca ggccgagatc gagacgatgg
     2341 cccgtcagct ggccgcgggc ggccacacac cgacggccga cccggccgag gccgacgtga
     2401 tcattctcaa cacctgcgcc gtgaccgccg cggccgcccg cgacgcccgc aacctgaccc
     2461 gccgcttcgc tcgcgctaac gcgtcggccg agatcgtcct gaccggctgc tacgccacgc
     2521 tggccccggc cgagctggcc gccctgcccg gcgtcgcccg tgttgtcgat aacgccagca
     2581 aggccagcat ccccctgctg ctcgacccgg ccctgcccac cgacgcgccc ctgttcgacc
     2641 gcgagccgct ggcccgcgaa acggccgccg gcagcatcgg ccgcacgcgc ggcttcatca
     2701 aagtccagga cggttgcgac aaccgctgca ccttctgcgt gaccaccatc gcccgcggcg
     2761 aggggcgcag ccggccgctg gccgaggtcg tggccgaagt gggcgcctgc gccgccgcgg
     2821 gctatcagga ggttgtcctc agcggcgtgc acctgggcag ctacggccgc gaccagggtc
     2881 gcccggccgg cctgcgcgaa ctggtcgccg cgctgctggc cgacaccgac atcccgcgca
     2941 tccgcctgtc gtcgctggag ccgtgggacg tcgcccccgg cttcttcgac ctgtgggcca
     3001 atccgcgcct gttgccccac ctgcacctgc cgctgcaatc gggcagcgac cgcgtgctgc
     3061 ggcgcatggc gcgacggaca acgcgcgccg ccttccgcgc cctgaccgac gaggcccgcg
     3121 ccgccatccc cgacctgaac ctgagcaccg acctgatcac cggcttcccc ggcgaaaccg
     3181 acgccgacca cgccgcgacg ctggcctatg tcgaagcgct gggcttcggc cgcctccacg
     3241 tcttcagcta cagcccccgc cccggcacgg ccgcggcccg aatgcccgac caggtttccg
     3301 gcccggttaa gaagtcccgc gcggcggcgc tgatcgccct gggagagcgg ctgggcgcgg
     3361 ccttccacac cggctacgac ggcgcgacac ggccggtgtt gtgggagatg gccaccggcg
     3421 cggaggcgaa tgggctgcgc tgggtcggct acaccgacaa ctacatccgc gtgaccgcca
     3481 ccggcccgac cggcctgatg aaccgtgtca cccttgcccg gctgagtagg ccgcgcgccg
     3541 acgggatggc cggcgagatc gatccggcca gcgcttccaa cagcgacagt ggggaagaat
     3601 gaatagaatg gacaatcaga aggagttgtc gcccgaacag cgcgcacaac tgctgaaaac
     3661 actgcaagcc cgttttgaaa agcacatgaa ccgccataac ggtcttgaat gggctgccgt
     3721 gcaggccaga ctaaacgcca atcctggaaa actgtggtcg ctcagcgaaa tggaacgaac
     3781 cggcggcgaa ccggatgtcg tcgggcaaga tgagcagacg ggcgcataca tcttctatga
     3841 ctgcgccgcc gaaagcccca aaaatcgccg tagcctctgc tatgatcctg aagcgctgtg
     3901 gagcagaaaa gagaacaagc cagccgacag cgccgtgggg atggcgagcg atatggggat
     3961 tgaactgcta acggaaggac agtatcgtgc cctgcaggag cttgagcgct tcgacacaaa
     4021 aacatcgagc tggctcaaga cccctgctaa catccggcaa ctgggcgggg cgatcttcgc
     4081 cgattaccgt tatggtcacg tgttcgttta ccacaacggc gccgaatctt actacgctgc
     4141 caggggcttc cgtggctcgc tgaaggtcta gcccgaccgc ccccgcgcct tcgcgcgcaa
     4201 cacctcatcc acccccggca gcggcgtgcg cagcaagcgc ccggccaggc tgacatccag
     4261 cgcgcaatcc aacggccagc gcccgctggc gtcttcggcc agagtcagcg tcgcccgttc
     4321 gcccacgccc caccagtcca gcaaccgcag ggcaaacgcg gcccgcgtca gcgtctgccg
     4381 cccggcaacg ttgagcacgc cggtatacgc ctggtcaacc agttccaggc aggccgcggc
     4441 cagcgtctcc acccagaccg ggttgcggcg ctggttggtg aataacgtca ccggttgccc
     4501 ggcccgcagc gccgcggcca tccacgccgt gccgttgtcc atctcctcca ggccgtagat
     4561 gagcgaggtg cgcacagcca cggcgttggc gtgggccagg gccaacgctt cggcctcagc
     4621 tttagcccgg ccgtagtcgt tgcgcggcgc gggcggggca ctctcgtcgt agggggccgc
     4681 gtcgccgcca aagagggcgt cggttgacag atggacgagc cgcgccccgg cttgtgtcgc
     4741 cgccgcagcg atgtgggccg tgccggcgac gatgacggcg ctcatatcgg gcgtgcggtt
     4801 ggagccggcg gtgtggatga tggcgtcggg ggcgaaatcg gccacgacgc gcgccacggc
     4861 cgcggcgtcg cgcaggtcga ggcgcgtgct ctgtggcaac ctcagcgggt cggccgagaa
     4921 gattgtatag cgccactccc ccggccaccc ggccgccacc cgccgcgcca gatgccgccc
     4981 cagatagccg ctgccgccgg tgatcagcaa tcgtttggac atagcgcgcc gcctgggatt
     5041 aaagaagctc acgcaaagga cgctaagcgg gctgaggacg caaagaagag accgttatct
     5101 tgttcttctc agcgcacttt gcttccttag cgtcctttgc gtgagcctct tactcctccg
     5161 gcgtggtctc ggcccggcgc atcgcctcgg cggcggcggc ctgggcggcg atctgcttgc
     5221 tgcggccgcg cccttcgccc cagacctctg ggccgaccag cacctgcacg gtaaacgtcc
     5281 gggcatggtc tggtccctcg ctgccgctga cgcggtagtg gggcgtgatg ttgtagcgcg
     5341 cctgcgccca gacctggaac tcgctcttgg cgtccttata tagctcgccg gcgcgaatgt
     5401 gctccagcgc cggacggacg agcgggtcga tgaacgcccg cgcccgctcc agcccgtggt
     5461 cgaggtagat ggccccgacg acagcctcga acgtggcgca aagcagcggc gtgcgttccc
     5521 ggccgccgtt ctcctcctcg cccaggccca gccgcagata gcgccccagg tcgatctgcc
     5581 gcgcgaaggc cgccagcgac tctgctcgca ccagcgccgc ccgcagcgct gtcaactcgc
     5641 cctcgtccat ttccgggaaa tgatgataga ggaagccggc gacgacgaaa tcgatcaccg
     5701 cgtcgcccag gaactccagc cgctcgttgt cctccagcac cgagccgggg ttctcgttca
     5761 ggtaggagcg gtgggtcagg gcgcgtgaga gcagcgacac atcgcgaaac tccacgccca
     5821 gttgttcact gaaagttagt aggtcgtcca tgattgcttg ctttcaccgc gcagggaagc
     5881 gaggcggccg gggagcgaag gcgcgctcgc gcttccccgc tctctgacgc attgagaagc
     5941 tactcctcaa acgcgcgaat cgccagcacg gcattgtgcc cgccaaagcc gaaggcgttg
     6001 ttcaggcaga cgcgcacgcg ccgcggccgg gccgcgttgg gtacatagtc caggtcgcag
     6061 gtcgggtcgg gcgtctcgta gtggatcgtt ggcggcgcga tctggtcgcg aatggccagt
     6121 gcacagaata cgctctcgat ggcccccgtc gcgcccatga tgtggccggt catggacttg
     6181 gtcgaactga tggcgacgtt gtacgcatgt tcgcccaggg cgctcttcac cgccgccgtc
     6241 tcggccgtgt cgttgagcgg cgtggccgtg ccgtgggcgc tgacgtaatc gacatcctcc
     6301 ggccgcagac cagcgtcggc cagcgccttg cggatggccc gcgccgcgcc gttgccgttc
     6361 tctggcgggg cggtgatgtg ataggcgtcg gtcgcctgcc catagccggc gatctccgcc
     6421 aggatggtcg cgccgcgcgc ccgggcatgc tccaggctct ccagaaccag gatacccgcc
     6481 ccttcgccaa tcaccaggcc gtcgcggtcg gcgtcaaagg gccgcggcgt atccgtcgcc
     6541 cgcgtcgagg tcgcgccggc gcgctcgaag ccaccaatgg ccaccgaggt caaaatcgac
     6601 tcagagccac cggtcagcac agcgtccagc tcgccggcgc ggatgctgcg gaaggcgtgg
     6661 ccaacggcgt cgttgccggc cgcgcaggcg gtagtgatgg tcgtcgatgg gccggtgatg
     6721 ccgtagtcaa tggccagcat gcccgccgcg ccgttgggca tgatcatggt gatgccaaac
     6781 gggctgagcc ggcgcaggcc gccgtcgtgg acgatggcct cctgctcgat gatggtgcgc
     6841 atcccaccga tgcccgtgcc cagggtgatg ccgacgcgct cgcggttgtc gtcggtgatg
     6901 gccaggccgg ccccgcgcat ggcctcggcc gcggccgccg tcgccagctg ctgatagcgg
     6961 tcgcgccgcc gcgcgtcctt gcggtccatg tactgcgccg ggtcaaactc cttgacttcg
     7021 cacagaccct gaatgatgtg ctgtgggtta tcgaccaggg tgatggggcc gaagcccgac
     7081 cgcccggcca ggatggccg
//
LOCUS       DAOSMQ010000112         7097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10613_length_7097_cov_4.670264, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000112 DAOSMQ010000000
VERSION     DAOSMQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10613_length_7097_cov_4.670264"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK829_05250"
     CDS             complement(<1..354)
                     /locus_tag="PK829_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU40654.1"
                     /translation="MSDAARAYARVHGEQFRQELHEMLRIPSLSADPAHTGDVRRMAE
                     WLADHFNSLGLDKVAVMETAGHPVVYGEWTGAGPDKPTVLVYGHYDVVPAQMEDGWHT
                     DPFEPVKKDGKIYARG"
     gene            complement(945..2510)
                     /gene="malQ"
                     /locus_tag="PK829_05255"
     CDS             complement(945..2510)
                     /gene="malQ"
                     /locus_tag="PK829_05255"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU40655.1"
                     /translation="MKFERAAGVLLHPTSLPSRYGIGDLGDGAYDFVDFLEAAKQQLW
                     QVLPLGPTGYGDSPYQSFSAFAGNPLLISPDRLVAEGYLPAEAVEATPTLPTAQVDYG
                     PVIEYKGELMRAAYEHFRAHGTAEQRELFDAFCANTAYWLDDFALFMALKEHHKDQDG
                     GVWNKWPSDIARRQKRAMKHWAGELSAAVELQKFQQFLFYKQWLELKAYANSKGIRII
                     GDIPIFVAYDSADVWSHPDLFYLNKDGSPSVVAGVPPDYFSETGQRWGNPLYRWERLA
                     ADGYSWWAQRIHMNLVQADIARIDHFRGFEAYWEIPGTEATAVKGQWIKGPGIPFFEA
                     IQARMGDLPIIAEDLGVITPEVEELRDRFQFPGMKILQFAFGGEHNSGFLPFNYVPNS
                     VVYTGTHDNETTLGWYLNATKDERDHARRYMRVSGQDVVWDLIRLAYASVSVMAIIPM
                     QDLFVLGNEARMNFPGREGGWWQWRYTREMFAARAPGIAVGLGELARLYGRVPEEPEE
                     EEEEEPEENPATP"
     gene            complement(2588..5449)
                     /locus_tag="PK829_05260"
     CDS             complement(2588..5449)
                     /locus_tag="PK829_05260"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU40656.1"
                     /translation="MPSTVARFFLQNPVTTGFTITDPFNSPRPYANGRHEGIDLRANQ
                     GGRPAEIVAAQAGVVHALHTGDTGYGNYARLRHDWADGTTWYTWYAHLSVINPALQVG
                     QPVEIGQRLGTAGTTGNSTGVHLHLTLQCIGGGLSGYVVADVVDPTRYFTSVAVPTID
                     ELTFLADVTAPDGSALTAGRAFTKTWRVGNTGTSQWSGYTLEHIGDEGMDGPASVPLP
                     ALAPDEVGEVSVPLVAPAEPGRHRSTWKACNAQGRRFAFELYADILVTPLARRDDAVL
                     VADLPLPGGTSVPAAGKALKAWRVRNTGDAPWGVGYHLAPDRAHDGAAVALPLVRPGA
                     TADLTITFSAPAETGVTRSVWRLRSPAGEPFGPELVIDVRVVAAPGRARPAATFVGDV
                     SVPDGTRLKPGQTFSKTWRLRNGGASAWSQGFTLAPADDNPFGGPAAVPLPPVEPGRT
                     ADITAELTAPATLGHHRAAWQPRAADGAPFGDIVHAAIEVVRPGAYDNARFEADVTYP
                     DGTVVPAGTRIDKTWRVRNTGMSAWAAGYALVFVAGNPMNGRSRTADSVPLPAALPGE
                     TVDVAVPLEAPLAPGVHRSSWRARNPEGMLFGDTLYAEIRVPVSSTSGSTAIDDAQLE
                     RHVTAPDGSEARAGAPFRKTWAIRNTGSTTWTDGYELVFAGGESMGQARRVPVGRVAP
                     QEVVEVSVDLTAPATPGRAVGRWRMHNGRGQAFGSTIFVAVAVVTAPTKIDLLPYLRG
                     DGRLYEMKYIFEMPNGPLIGQQRLQTQYDSERFYQTKDSEWEELWADDRFIYRGTDTS
                     PGSGNFYTLMDGDRYGTAWVPRHMAVGQTYRRSVAVISRRKGNCVVNSHLSGRHVTWI
                     RLEAVHDTLTLPDVEGRPGRGLVVTDAVVLAAYSEANGRPTEQPFERYTYAKGYGLVL
                     WQGIATDHKGSAFLVQLHKPGDRPDNVRERIPCLEEIRN"
     gene            complement(5453..6904)
                     /locus_tag="PK829_05265"
     CDS             complement(5453..6904)
                     /locus_tag="PK829_05265"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOU40657.1"
                     /translation="MADPLRILFLSAEVAPFAKTGGLGDIGGSLPKALQAMGHDVRVI
                     MPAYHNIEHGYPGVSSMPLQLLVPTGSGTIGAGAFEGRLPGSDVPVYFIAEYNLFGRP
                     NIYGYWDDAYRFAFFSKAALELTLALDWKPDILHAHDWHTAPAVTWLHTAGATDPRFQ
                     GIGSVFTIHNLAHQGRTSWNVFDYMQLFTHGLSEEAYGEVNFMARGIYHANIVNTVSP
                     TYAREIMTREGGAGLDGLLRHRQRDLHGILNGLDYDEWNPGTDKRLAASYGLDDMAGR
                     DENRRALQAAVGLPQLANIPIIGMVTRLDWQKGLDIVGHSLHLLMNGYSGPAQFVVLG
                     SGAPQYEDMFRQMAGYHREKMSAYIGYHAELAPLIYAGSDMFLMPSLFEPCGLGQMIA
                     MRYGSVPIVRATGGLADTVQDGITGYSFYDFNTDALFHAIQRATYTYNTDRDSWRRIQ
                     ANGMTNNFSWERSAQGYEQLYRWAQAQVGHFLG"
BASE COUNT         1134 a         2189 c         2495 g         1279 t
ORIGIN      
        1 gccgcgggcg tagattttgc cgtctttttt gaccggctca aaggggtcgg tgtgccagcc
       61 gtcttccatc tgggcgggga cgacatcgta gtggccgtag acgaggacgg tcggcttgtc
      121 ggggccggcc ccggtccact cgccatagac aacggggtgg ccagcggtct ccatcaccgc
      181 cactttgtcc aaccccaggc tattgaaatg gtcggccagc cactcggcca tgcgacgaac
      241 gtcgccggtg tgggcggggt cagcgctcag gctgggaatc cgcagcattt catgcagttc
      301 ctggcggaac tgctcgccgt ggacgcgggc gtatgcgcgg gcagcgtcac tcattgcagt
      361 catgtcttgg cacactcctt tcgattcttc agtgttgtca gcagtattcg gcaagacaag
      421 cgcatcaggc tgccagtgtg tatttcgccc tacctacaat cgccttagac cgagcgggtc
      481 tattctgtga taagctatgg taaagcgctc ggagaaagtc cgaactgggg cttattggtg
      541 ggccgttcca aaatgtcacc gtcgggaata cttggagacg atgtccattt tggagggcgc
      601 ctgatggccc aattacctaa gacaattatc ttatcatttc gggtgagtgt caatcgtttc
      661 tcaaagtcaa ccccaggcgc gccgaactga caggtgggcg accagtcgtt ctgtggtcgc
      721 ccacctgtca gttcattggc aaagcccggc agaggcgacc ggcggggctt ctggcggggc
      781 ctctggcgag gccacgcctc gtctacgtgc tcattgggtt aggacgtact gccgcgtgag
      841 acattgtgcc gcagtttcaa ggccggcgat ccggaccatg agcctgggcc agttcggccg
      901 ggacgatcac ttgtcgcccc ggccacgttt cgggatgctc ccggtcaggg cgtcgccggg
      961 ttttcttccg gctcctcctc ttcctcctcc tctggctctt ccggcacgcg gccgtagagc
     1021 ctggccagtt cgcccagacc cacagcgatg ccaggcgcgc gggcggcgaa catctcgcgc
     1081 gtgtagcgcc actgccacca acccccctcg cggccgggga agttcatgcg cgcctcgttg
     1141 cccagcacaa acaggtcttg catgggaatg atggccatga ccgacaccga ggcataggcc
     1201 agccggatca ggtcccagac cacatcctgg ccgctgacgc gcatgtagcg ccgggcgtgg
     1261 tcgcgctcgt ctttggtggc gttcagatac cagcccagcg tcgtctcgtt gtcgtgcgtg
     1321 ccggtgtaga ccacggaatt gggcacgtag ttgaacggca ggaagccgct gttgtgttcg
     1381 ccgccaaagg cgaactggag gatcttcatg ccggggaact ggaagcggtc gcgcaactct
     1441 tcaacctccg gcgtgatcac gcccaggtct tcggcgatga tgggcaggtc gcccatgcgc
     1501 gcctgaatcg cctcgaagaa gggaatgccc ggccccttga tccactggcc cttaacggcc
     1561 gtggcctctg tgcccggaat ttcccaatag gcctcgaagc cgcggaagtg gtcgatgcgg
     1621 gcgatgtcgg cctgcaccag gttcatgtgg atgcgctgcg cccaccagct atagccgtcg
     1681 gcggccaggc gctcccagcg gtagagcggg ttgccccagc gctggccggt ctcgctgaag
     1741 tagtccggcg gcacgccggc cacgaccgaa ggcgaaccgt ctttgttcag gtagaacagg
     1801 tcggggtgcg accagacgtc ggcgctgtcg taggcgacga agatgggaat gtcgccgatg
     1861 atgcggatgc ccttgctgtt ggcgtaggct ttcagctcca gccactgctt atagaacaga
     1921 aattgttgga acttctgcaa ctcaacggcc gcgcttagct cgccggccca gtgcttcatg
     1981 gcccgcttct gtcgcctggc gatgtcgctc ggccacttgt tccagacgcc gccgtcctgg
     2041 tctttgtggt gttccttgag ggccatgaac agagcgaagt cgtcgagcca gtaggccgtg
     2101 ttggcgcaga aggcgtcgaa caactcgcgc tgctcggctg tgccgtgggc acggaaatgc
     2161 tcataggccg cccgcatcaa ctcgcccttg tactcgataa ccggaccgta atcgacctgg
     2221 gccgtgggca gtgtcggcgt ggcttcgacc gcctcggcag gcagatagcc ctcggctacc
     2281 agccggtcgg ggctgatgag cagcgggttg ccggcaaagg ctgagaagga ctggtaaggc
     2341 gagtcgccat agcccgtggg gccgaggggc aacacctgcc aaagctgttg cttggctgcc
     2401 tccaggaaat cgacgaagtc ataggctccg tcgcccaggt cgccgatgcc gtagcggctg
     2461 ggcaaactgg tggggtggag caggacaccg gcagcgcgtt caaatttcat ggggtctcct
     2521 tgttgggtca gtaggcccgt aggtcagtgg tcgatggtca gtggtcagtg gtcagttgtt
     2581 acccttctta atttctaatt tcttccaggc agggaatgcg ctcgcgcaca ttatccggcc
     2641 gatcgccggg tttgtgtagt tgaacgagga aggcgctgcc cttatggtcg gtggcgatgc
     2701 cttgccagag caccaggccg tatcccttcg cataggtata gcgctcgaac ggttgctcag
     2761 tcgggcggcc gttggcctcg ctataagcgg ccagcaccac tgcatccgtc acgaccaggc
     2821 cgcggcccgg ccgcccttcg acatcgggca gcgtcagcgt gtcgtggaca gcctccagcc
     2881 gaatccaggt cacgtggcgg ccggagaggt gggaattgac gacacagttg cccttgcgcc
     2941 ggctaatgac ggcgacgctg cgccggtagg tctggcccac ggccatatgg cgcggaaccc
     3001 aggccgtgcc atagcggtcg ccgtccatga gcgtatagaa gttgccggag ccgggcgagg
     3061 tgtccgtgcc gcggtagatg aagcggtcgt cggcccacag ttcttcccac tcgctgtctt
     3121 tggtctgata gaagcgctcg ctgtcgtatt gggtctgcaa gcgctgctgg ccaatgagcg
     3181 ggccgttggg catctcgaag atgtacttca tctcgtagag ccgcccgtcg ccgcgcagat
     3241 agggcagcag gtcgatcttc gtcggcgcgg tcacgacggc gacggcgacg aagatagtcg
     3301 agccaaaggc ttggccgcgg ccattgtgca tgcgccagcg gccgacggcc cgccccggcg
     3361 tggccggcgc ggtcaggtca acgctgacct cgacaacctc ctgcggggcc acgcggccga
     3421 cgggcacgcg ccgcgcctgg cccatgctct cgccgccggc gaagacgagt tcatagccgt
     3481 ccgtccacgt cgtcgagccc gtgttgcgaa tagcccaggt tttgcggaag ggcgcgccgg
     3541 cccgcgcctc gctgccgtcg ggcgcggtga cgtggcgctc caactgggcg tcgtcgatgg
     3601 ccgtcgagcc gctggtggag gaaacgggca cgcggatctc ggcgtagagc gtgtcgccga
     3661 agagcatgcc ctccgggttt ctggcccgcc aggagctgcg gtggacgcca ggggccaggg
     3721 gcgcttccag cggcacggcg acgtcgactg tttcgcccgg cagcgccgcg ggcaaaggaa
     3781 cgctgtcggc cgtgcggctg cggccgttca tggggttgcc ggcgacgaag acgagggcgt
     3841 agccggccgc ccaggccgac atgccggtgt tgcgcacgcg ccaggttttg tcgatgcgcg
     3901 tgcccgcggg cacgaccgtg ccgtcggggt aggtgacatc agcttcgaag cgggcgttgt
     3961 catacgcgcc ggggcgcacg acctcgatgg cggcgtgaac gatgtcgccg aagggtgcgc
     4021 cgtcggcggc gcggggctgc caggcggcgc ggtggtggcc gagggtcgcc ggggcggtta
     4081 gttcggcggt gatgtcggcc gtgcgacccg gctccaccgg cggcaagggc acggccgccg
     4141 ggccgccaaa ggggttgtcg tcggccgggg ccagcgtgaa accctggctc caggccgacg
     4201 cgccgccgtt gcgcaagcgc cacgttttgc tgaaggtctg gcccggctta aggcgcgtgc
     4261 cgtcgggcac agaaacatcg cccacaaagg tcgccgccgg gcgggcgcgg ccgggcgcgg
     4321 cgacgacgcg cacgtcgatg accagttccg ggccaaacgg ttcgccagca gggctgcgca
     4381 gccgccagac ggaacgggtg acgcccgttt cggccggggc gctgaaggtg atggtcaggt
     4441 cggctgtggc ccccggccga acgagaggca gagccacagc cgcgccgtcg tgggcgcggt
     4501 cgggggccag gtggtagccc acgccccagg gtgcgtcgcc agtgttgcgc acgcgccagg
     4561 ccttcagggc ctttcccgcc gcgggcacgc tcgtcccacc gggcaggggc aggtcggcga
     4621 cgagaacggc atcgtcgcgc cgggccaggg gcgttaccag gatgtcggca tacagctcga
     4681 aggcgaagcg gcggccctgg gcattgcacg ccttccaggt gctgcggtgg cggcccggct
     4741 ccgccggcgc gaccaggggc acgctgacct cgcccacttc gtccggggcc agcgccggca
     4801 gcggcacgct cgctggtccg tccatgccct catcgccgat gtgctccagc gtgtagcccg
     4861 accactgcga cgtgccggtg ttgcccacgc gccaggtttt ggtgaaggcg cgcccggccg
     4921 tcagcgccga gccgtccggc gccgtcacgt cggccagaaa ggtcaactcg tcgatggtcg
     4981 gcacggccac gctggtgaag tagcgcgttg ggtcaaccac gtcggcgacg acgtagccgc
     5041 tcagaccgcc gccgatgcat tgcagcgtca ggtgcagatg cacgccggtg gaattgccgg
     5101 tcgtgccggc cgtgcccagc cgctggccga tctcgaccgg ctggccgact tgcagggcgg
     5161 ggttgatgac cgacaggtgg gcgtaccacg tgtaccaggt cgtgccgtcg gcccagtcgt
     5221 ggcgcaggcg cgcgtaattg ccgtagcccg tgtcgccggt gtggagggca tggacaacgc
     5281 cggcctgcgc agcgacgatc tccgccggcc ggccgccctg gttggcccgc aggtcaatgc
     5341 cctcgtggcg gccgttggca taggggcgcg gactgttgaa gggatcggtg atagtgaacc
     5401 cggtcgtgac tgggttttgc aggaagaagc gagcgaccgt cgaaggcatg gcttagccga
     5461 gaaagtggcc cacctgggcc tgcgcccagc ggtagagttg ctcatacccc tgagccgagc
     5521 gctcccagga gaagttgttg gtcatgccgt tggcctggat acgccgccag ctgtcgcggt
     5581 cggtgttgta ggtgtaggta gcgcgctgga tggcgtggaa cagggcgtcg gtgttgaagt
     5641 cgtagaacga gtagccggtg atgccgtcct ggacggtatc ggccaggccg ccggtggcgc
     5701 ggacgatggg cacgctgcca tagcgcatgg cgatcatctg ccccaggccg cacggctcaa
     5761 agagcgacgg catcaggaac atgtcgctac cggcgtagat gagcggcgct aactcggcat
     5821 ggtagccgat gtaggccgac atcttctcgc ggtggtagcc ggccatctgg cggaacatgt
     5881 cctcgtattg tggcgcgccg gaacccagga cgacgaactg cgctggcccg gagtagccgt
     5941 tcattagcag gtgcaggcta tggccaacga tgtccagccc cttctgccaa tcgagccggg
     6001 tgaccatgcc gatgatgggg atgttagcca attgtggcaa gccgacggcg gcttgcaggg
     6061 cgcggcggtt ctcgtcgcgg ccggccatgt catcgaggcc gtagctggcc gccaggcgct
     6121 tgtctgtgcc ggggttccac tcgtcgtagt ccagcccatt gaggatgccg tgcaggtcgc
     6181 gctggcgatg gcgcagcagg ccgtcgaggc ccgcgccgcc ctcgcgggtc atgatctcgc
     6241 gcgcataggt ggggctgacg gtgttgacga tattggcatg gtagatgcca cgggccatga
     6301 agttgacctc gccataggct tcttctgaca gcccgtgggt gaacaactgc atgtagtcga
     6361 agacattcca gctcgttctg ccctggtggg ccagattgtg gatggtgaag acgctgccga
     6421 tgccctggaa gcgcgggtcg gtcgcgccgg ccgtgtggag ccaggtcacg gccggggcgg
     6481 tgtgccagtc gtgggcgtgg aggatgtcgg gcttccagtc tagcgccagg gtcagctcaa
     6541 gagccgcctt gctgaagaag gcaaagcggt aggcgtcgtc ccagtagccg tagatgttgg
     6601 gccggccgaa gaggttgtac tcggcgatga agtagacggg cacatcgctg cccggcaagc
     6661 ggccctcgaa cgcgcccgcg ccgatggtgc cggagccggt tggcaccagc agttgcaggg
     6721 gcatgctgct gacccccgga tagccgtgct cgatattgtg gtaggccggc atgatgacgc
     6781 gaacgtcgtg gcccatcgcc tggagcgcct tcggcagcga gccaccgatg tcgcccaggc
     6841 cgccggtctt ggcgaaggga gcaacttcgg cgctgagaaa gagaattcgg agtggatcgg
     6901 ccatgtcgtg cctcgcgcaa gtggcttgga tgatggatga ttcagtttgg ttgactgcgg
     6961 gtggcgttcg cgtcgccagc ggactgacta tattgtgccc aattgccggc cgatggacaa
     7021 atagatgtgg cgcgattctc ccgaatcgcg ctcacggccg cccgcgctca cgcccgcgat
     7081 tcgggagaat cgcgcca
//
LOCUS       DAOSMQ010000113         7090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10635_length_7090_cov_4.499787, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000113 DAOSMQ010000000
VERSION     DAOSMQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10635_length_7090_cov_4.499787"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="PK829_05270"
     CDS             <1..1786
                     /locus_tag="PK829_05270"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU40658.1"
                     /translation="STYGTGAIMAVPAHDQRDFDFARQYGLEIIPVIQPEGERLDGAT
                     MTAAYVGPGVMINSAEFNGTPVTAAKGFANPSIAAVTGWLAERGAGGQSINYRLRDWL
                     ISRQRYWGAPIPALFCADGTIEMVPDDALPVLLPDDIEFMPTGQSPLRTHEGFLNAAD
                     AEGQPARRETDTMDTFMCSSWYQYRYLSPRYDRGPFDPEEAAYWLPVDVYTGGAEHAT
                     MHLLYTRFFTKVMRDLGLFEETARAMTAHGRETEGQFDEPMMTLRNQGQILGAERKGD
                     VIVAYGETAGNKMVARHVEVIAPGARPVGRRNDEVVGEIMKRTENVLTVDVGDELFTV
                     EVSPTAVVAIPSIEGDNNVNQLRHHLEVQRMSKSKGNVVNPDELVAQYGADTVRAYLM
                     FFAKWDQGGPWNYEGIKGPQRLLQDVWEFAQAQYPGGDDEAASRGLRRKTHQVIRKVT
                     QDLQDFSFNTAVAAMMELRNTIIAAQREGGANRAAWEEAVESLLLLLAPVSPHITEEL
                     WARRGLPYSIHHQPWPQWDDDVAREEMISLIVQINGKTRDRIDVAAGLPDEELRAIAL
                     ASDKVSRWLDGKTPQRIIVRGQLVNVVV"
     gene            complement(1868..2497)
                     /locus_tag="PK829_05275"
     CDS             complement(1868..2497)
                     /locus_tag="PK829_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOU40659.1"
                     /translation="MLPYLLRGLILGGTAAAQPGPFQAFLLSLVARDGWRRALPAVLA
                     PLVSDPPIVALVLVVLTRLPERWLAVLQVAGGVFLFYLGWGAWRAFRTWTAGDTASAP
                     ARGSNLAAILKAAVMNALSPGPYLFWATISGPILVAGWRERPVLGLAFLLGFYAALIG
                     GMVLFVLVVGLTGRIGPRLNRALAAFAVVALFGFGLYQFVTGVRALVEA"
     gene            complement(2511..3275)
                     /gene="map"
                     /locus_tag="PK829_05280"
     CDS             complement(2511..3275)
                     /gene="map"
                     /locus_tag="PK829_05280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU40660.1"
                     /translation="MRLKNAKEIARMRASGRLVAECFDILQDSIRPGISLKALDRLVE
                     NHIYKQGAETLYKGYRGRGREHPPFPGVICASVNHEICHGLPDERVLREGDVVGIDIG
                     LRYKGYCGDACVTYPVGQVSAETERLLAIGREALRLGIEAAQYNHHLNDIGRAIENYA
                     DTQGVTVVHEWGGHEIGRALHGDLSVSHIRQPGPGPRLIPGMTFTIEPMINAGTHEWL
                     MLPDGWTVITADGRNSVQFEHTIAITPNGPDILSRL"
     gene            3675..4970
                     /locus_tag="PK829_05285"
     CDS             3675..4970
                     /locus_tag="PK829_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU40661.1"
                     /translation="MVDESSTEARSLRPFLTLWIGQVFSLLGSQLVQFALIWWLTQQT
                     GSATVLAIASIVGIVPQVVLGPFVGPLVDRWNRRWTMILADGAVAAATAVLAILFWLG
                     LIAPWHVYVILFVRAVGGTFHGPAMAASTALMVPEAYLTRIQGLNQMLNGGLSVIAAP
                     LGALLLELLPLQGVLLVDIITAAIAITTVLLVHVPQPRHATTEVHGSAARQYWQDLRA
                     GLRYVLSWRGLLILMVMATFINLVLSPSMSFLPLLVTKHFQGTAWHLGALEAGLGVGV
                     IAGGVLLGVWGGFQRRIYTSLAGLIGLGLSVLLIGLVPASLFPLAVIAMGLMGMMSAM
                     TNGPLMAVMQATVDPAMQGRVFTLLSSAATAMMPLGLALAGPLADVIGVRTWFLLGGL
                     VTLAIGVGGFFFRSLASLEQDRHNPGAPVPTALPTTTAD"
     gene            complement(5008..5388)
                     /locus_tag="PK829_05290"
     CDS             complement(5008..5388)
                     /locus_tag="PK829_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40662.1"
                     /translation="MSTLVNVHHALSNTAWLFFLILGLWGLFRAIRGQSVNANYLGAA
                     FIGQLIFVAQAIMGGVLWLGGGSAQIARPDIHLLYGVFALIFLPFIYVSVLRGDDTNR
                     AQWVLAFTTLFLFGIALRSITTSI"
     gene            5726..5938
                     /locus_tag="PK829_05295"
                     /pseudo
     CDS             5726..5938
                     /locus_tag="PK829_05295"
                     /inference="COORDINATES: protein motif:HMM:NF021758.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(5935..6699)
                     /locus_tag="PK829_05300"
     CDS             complement(5935..6699)
                     /locus_tag="PK829_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOU40663.1"
                     /translation="MNVIIVGETPFVDEIGRLCRAAGHVTERLPGRAFLAEGQAGDER
                     QKLAAAEVVIAAQDTSAAAKRAVLMALSVGVPEEALLLTLALATSVTTAAAWVRSPER
                     VVGFGVVPPLPDEGIVELAAALQTTTGALAQAEEFWRGLKQEPVRVADGPGLVRARTV
                     CCLINEAASVLMEEVAVAADIDLAMRLGTNYPHGPLAWADRIGLDVVLGVMTGLFDEW
                     GDDHYRPAPLLRRMVAAGWLGRKSGRGFYLYPDEAG"
     gene            complement(6696..>7090)
                     /locus_tag="PK829_05305"
     CDS             complement(6696..>7090)
                     /locus_tag="PK829_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU40664.1"
                     /translation="RLFNPERTAHFLGPRLFPKPEQAQLREMFIAQWAQNDPQAYRAA
                     MRALVGWSVLDRVSRIACPTLVISGDRDYTPPATKAAYVAMMPNARLVVLADSGHATP
                     IDQAGRFNDCVLAFLASVAAPERDEERV"
BASE COUNT         1263 a         2350 c         2310 g         1167 t
ORIGIN      
        1 cagcacctat ggcactggag cgatcatggc tgtgcccgcc cacgaccagc gcgacttcga
       61 cttcgcccga cagtatggcc tggagatcat cccggtcatc cagccggagg gcgaacgcct
      121 cgacggggcg acgatgaccg cggcctatgt ggggccgggc gtcatgatca acagcgccga
      181 gtttaatggc acgcccgtca ccgccgccaa aggcttcgcc aatccctcca tcgctgccgt
      241 gaccggatgg ctggccgagc gcggcgccgg cggccagtcg atcaactacc gcctgcgcga
      301 ctggctcatc agccggcagc gctactgggg cgcgcccatc ccggccctgt tctgcgccga
      361 tggcacgatc gagatggtgc ccgacgacgc attgccggtg ttgctgcccg acgacatcga
      421 gttcatgccc accggccaaa gcccgctgcg cacccacgag ggcttcctga acgctgccga
      481 cgcggagggc caacccgccc gccgtgagac agacacgatg gacaccttta tgtgctcctc
      541 ctggtatcag taccgctatc tcagcccgcg ctacgaccgc ggcccattcg atccggaaga
      601 ggccgcctac tggctgccgg tcgacgtcta caccggcggg gccgagcacg ccaccatgca
      661 cctgctctac acgcgctttt tcaccaaagt gatgcgcgac ctgggcctgt tcgaggagac
      721 ggcccgcgcc atgactgccc acggccgtga aaccgagggc cagttcgacg aaccgatgat
      781 gaccttgcgc aaccagggcc agattctggg cgcggagcgc aagggagatg tcatcgtggc
      841 ctacggcgag acggcaggca acaagatggt cgcccgccat gtcgaggtca tcgccccagg
      901 ggcgcggccg gttggccggc gcaacgacga ggtcgtcggc gagatcatga agcgcaccga
      961 aaacgtgctg accgtcgatg ttggcgatga gctgttcacc gtcgaggtgt cgcctacggc
     1021 cgttgttgcc atccccagca ttgagggtga caacaacgtc aaccagttgc gtcatcacct
     1081 ggaagtccag cgcatgtcca agagcaaggg caacgttgtc aaccccgacg agttggtggc
     1141 gcagtacggc gcggacacgg tgcgggccta cctgatgttc ttcgccaagt gggaccaggg
     1201 cgggccgtgg aactatgagg gcatcaaggg gccacagcga ctcttgcagg atgtctggga
     1261 gttcgcccag gcccagtatc cgggcggcga cgacgaggcc gcctcgcgcg gcctgcggcg
     1321 caagacccac caggtcatcc gcaaggtgac gcaggacttg caggacttct cgttcaacac
     1381 ggccgtggcg gccatgatgg agctgcgcaa caccatcatc gccgcccagc gcgagggcgg
     1441 cgccaatcgc gccgcctggg aggaggccgt cgagtcgctg ttgctactgc tagcgccagt
     1501 gtcgccccac atcaccgagg agctgtgggc gcggcgcggc ctaccctata gcatccacca
     1561 ccagccctgg ccacaatggg acgacgacgt ggcccgcgag gagatgatca gcctgattgt
     1621 gcaaatcaac ggcaaaaccc gcgaccgcat cgacgtggcc gcgggactgc ccgacgaaga
     1681 gttgcgcgcc attgctttgg cgtcggacaa ggtcagccgc tggctggatg gcaagacgcc
     1741 ccagcgtatc atcgtccgcg gccaattggt caacgttgtc gtctagcggc tgcgccggcg
     1801 gtgtggtcaa agaagcggcg gcgcggttgg ccttgttggg cgggccgcgc cgctttttgt
     1861 tgccgcgtca ggcctcgacc agggcacgca cgccggtcac gaactggtag aggccaaagc
     1921 cgaacagggc tacgacggca aaggcagcca gagcgcgatt gaggcggggg ccgatgcggc
     1981 cggtcagccc gacgacgagg acgaacagaa ccatgccgcc gatcagggcc gcgtagaagc
     2041 ccaataggaa ggccagcccc agcaccggcc gctcgcgcca gccggccacc agaatggggc
     2101 cgctgatggt tgcccagaac aggtacggcc ccgggctgag ggcgttcatg accgccgcct
     2161 tgaggatggc cgccaggttg ctgccgcgag ccggcgcgct ggccgtgtcg ccggccgtcc
     2221 acgtccggaa cgcccgccac gccccccagc cgaggtagaa cagaaagaca ccgccggcca
     2281 cctggagcac ggccagccag cgctccggca ggcgcgtcag caccaccagc accagggcaa
     2341 cgatgggcgg gtcgctgacc aggggggcca ggacggccgg cagcgcgcgc cgccagccat
     2401 cgcgggccac caacgatagc aggaacgcct ggaaagggcc gggctgcgcg gcggccgtgc
     2461 cgcccaggat gaggccacga aggagatagg gcagcacgga cgcagtgggg ctagaggcgg
     2521 ctcagaatgt ccgggccgtt gggggtgatg gcaatggtgt gttcaaattg caccgagttg
     2581 cgcccatcgg cggtgatcac cgtccagcca tcgggcaaca tcagccactc gtgcgtgccg
     2641 gcgttgatca tcggctcgat ggtgaaggtc ataccgggga tgaggcgcgg ccccgggcca
     2701 ggctggcgaa tgtgggacac ggacagatcg ccgtgcaagg cgcggccgat ttcgtggccg
     2761 ccccactcat ggacgacggt gacgccctgg gtgtcggcgt agttttcaat ggcccggccg
     2821 atgtcgttga gatggtgatt gtattgcgcc gcctcaatgc ccagtcgcag cgcctcgcgg
     2881 ccaatggcca acaggcgctc cgtctccgcc gaaacctggc cgacggggta ggtgacgcag
     2941 gcatcgccgc aatagccctt gtagcgcaga ccaatgtcga tgccaaccac gtccccctcg
     3001 cgcaggacgc gctcatcggg caggccgtga cagatctcgt ggttgaccga ggcgcagatg
     3061 acgccgggga agggcggatg ttcccggccg cggccgcggt agcccttata gagcgtctcc
     3121 gcgccctgct tatagatgtg gttctcaacg aggcgatcca gcgccttgag cgagatgccg
     3181 gggcggatac tgtcctggag gatgtcgaaa cattcggcga cgagccggcc agacgcgcgc
     3241 atccgggcga tttctttagc gttcttgagt ctcataatcg atcactgagt atatcacaaa
     3301 acgcaccggc ttgcgcagga cggctgtcga ccagcacggc ttttcagttg tcaccgcttg
     3361 agacctgacc ggctatatgc cagtggtaca gcctgtcagg ctgtcgttac cggccgcagc
     3421 ctgacacagc ctaacaggct gtgctacagg ctgtgctacg ggccgtggga ctactgaaaa
     3481 accctgtgcc gaccagagca tctcagcgaa gacctgacag gtgaccaata cagcgctggt
     3541 ggacgcaaaa gttgtggtca cctacctgtc aggtatgggc cagacaatct acgtctatgt
     3601 aaagattatt gacagaggta ttaataaaat gtatactttc cgcaataatc ctgcgatagg
     3661 tcggtgagac ccacatggta gacgaatctt caacagaagc ccgctcgttg cgcccctttc
     3721 tgacactgtg gattggccag gtgttttcgc tgctgggcag ccagttggtg cagtttgcgc
     3781 tcatctggtg gctgacacag cagaccggct cggccaccgt cctggccatc gcctccattg
     3841 tcggcatcgt tccccaggtg gttctcggcc cttttgtcgg cccgctggtg gatcgctgga
     3901 accggcgctg gacaatgatc ctggccgatg gcgcggtagc cgcggccacg gccgtcctgg
     3961 ctatcctgtt ctggctgggc ctgatcgcgc cgtggcacgt ctacgtcatc ctctttgttc
     4021 gcgcggtggg gggcaccttc cacggcccgg ccatggccgc ctcgacggcg ctgatggtgc
     4081 ccgaggcgta cttgacgcgc attcagggcc tcaaccagat gctcaacggc gggctgagtg
     4141 tcatcgccgc cccgctgggc gcgttgctgc tggaactgtt gccgctgcaa ggcgtgttac
     4201 tggtcgatat catcacggcg gccatagcca tcacgacggt tttgctggtt cacgtgccgc
     4261 agccgcgcca cgccacgacc gaagtccacg gctcggccgc ccgccagtat tggcaggact
     4321 tgcgcgccgg tctgcgctac gtcctcagtt ggcggggcct gctgatcttg atggtcatgg
     4381 ccacgttcat caatctggtg ctcagcccat caatgagctt tctgccgctg ctggtgacaa
     4441 agcacttcca gggcacggcc tggcacttgg gcgcgctgga ggccggactg ggcgtcggcg
     4501 tgatcgccgg cggcgtactg ctgggagtgt ggggcggctt ccagcggcgc atctatacat
     4561 cattggccgg cctgatcggg ctggggttga gcgtcctcct gatcggcctt gtgccggcgt
     4621 cgctctttcc gctggcggtt atcgcgatgg gcctgatggg gatgatgtcg gccatgacca
     4681 acgggccgct catggctgtc atgcaggcca ccgtagaccc ggccatgcag gggcgcgtct
     4741 tcacgctgct tagcagcgcg gccacggcga tgatgcccct cggcctggcg ctggccggac
     4801 cgctggccga cgtgatcggt gtgcggacgt ggttcttgct gggtgggttg gtcacgctgg
     4861 cgatcggcgt tggcggcttc ttcttccggt cgctggccag tctggagcag gatcgccaca
     4921 accccggtgc ccccgtcccc actgcgctgc ccacgacgac ggccgactag cgcaccgcga
     4981 ccgctcttcg ccgatgggcg ctcccattca gatcgacgtt gtgatcgacc gcagggcgat
     5041 gccgaacaga aatagcgtcg taaaggccag cacccactgg gcccgattgg tgtcgtcacc
     5101 gcgcaggaca gagacgtaga taaacggcaa aaagatcaag gcgaagacgc catagagcag
     5161 atgaatatct ggccgcgcta tctgcgcgct gccgccgccc agccagagga cgccgcccat
     5221 gatggcctga gcgacgaaga tgagctggcc aatgaaagcc gcgccgaggt agttggcgtt
     5281 aaccgactgg ccgcggatgg cacggaaaag cccccacagc cccagaatca ggaagaacag
     5341 ccacgccgtg ttagacagcg catggtggac attgacgagt gttgacatgg tgcgtgcctc
     5401 gatgaggcga gtgtagcagg gggaacggcc ttgtcaaagc ggccgggctt tccgtgcgcc
     5461 aatttttcta cgtgccatag ccctgtccac ttggccgcac cgcttgacca cgacttttcc
     5521 aaagtcaccc caggcgcacc gacctgacca gtggacggcc acctgtcagg tcaatgggcg
     5581 ctagacgact ttggaaaagc ccggccgctt gacgccagca cgcgccccgc gtactattcc
     5641 cccaacttca taggagccag ccatgcccaa gtacgtcatc agcctcgaat actgcgttcc
     5701 ctgaaactac tggccgcgcg ccgcccgtgt ggcggacgac attatgagca actaccagca
     5761 cgtcatcgaa cgcttcaccc tcatcaccgg tggcaagggc acgttcgtct tcaccgtcaa
     5821 tggcgaggta ctgttctcga agaaggccct cggccgccac gccgaaccgg gcgaagtgct
     5881 ggcgctgttc cggcaattca tcggcccgga cgtagagcct tacccgcgcg aactctaacc
     5941 cgcctcgtcg gggtagagat agaagccgcg gccgctcttg cgacccagcc agccggcggc
     6001 caccatccgc cgcagcagcg gcgcgggccg gtagtggtcg tcgccccact cgtcgaacag
     6061 gccggtcatc acgcccagaa caacgtccag gccgatgcgg tcggcccagg ccagcggccc
     6121 atgagggtag ttggtgccca ggcgcatggc caggtcgatg tcggcggcga cggcgacttc
     6181 ctccattagc acgctggcgg cctcattgat cagacagcag accgttcgcg cccgcaccag
     6241 ccccggcccg tccgccaccc gcaccggctc ctgcttcagg ccgcgccaga actcctccgc
     6301 ttgcgccaat gccccggtcg tggtctgtaa cgccgccgcc aactcgacga tgccctcgtc
     6361 tggcagaggc gggacaacgc cgaagccgac gacacgctcc ggcgaccgca cccaggcggc
     6421 cgcggtcgtg acgctcgtgg ccagggccaa cgtcagcagc agcgcctcct ccggcacgcc
     6481 aacgctcaga gccatcagca ccgcccgctt ggccgccgcc gacgtgtcct gcgcggcgat
     6541 gacgacctcg gccgcggcca gcttctggcg ctcgtcgcca gcctgcccct cggccaggaa
     6601 ggcgcgaccc ggcaaccgct ccgtgacgtg cccggccgcg cggcagagac ggccgatctc
     6661 gtctacaaac ggcgtctcac cgacgatgat gacgttcaca cgcgctcttc gtccctctcc
     6721 ggcgcagcca cgctggccag gaaggccagc acgcagtcgt taaagcgccc ggcctggtcg
     6781 atgggcgtgg cgtggccaga gtccgccagc acgaccagcc gcgcgttggg catcatggcc
     6841 acataggcgg ccttggtcgc cggcggcgta tagtcgcggt cgccggagat caccagcgtt
     6901 gggcaggcga ttctgcttac ccgatcgagg acgctccagc cgaccaacgc ccgcatggcc
     6961 gcgcggtaag cctgcgggtc gttctgcgcc cattgagcga tgaacatctc ccgcaactgc
     7021 gcctgctccg gcttggggaa gaggcgcggg ccgaggaagt gggccgtgcg ctccgggttg
     7081 aacaggcggg
//
LOCUS       DAOSMQ010000114         7084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10650_length_7084_cov_4.797980, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000114 DAOSMQ010000000
VERSION     DAOSMQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10650_length_7084_cov_4.797980"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="sppA"
                     /locus_tag="PK829_05310"
     CDS             <1..697
                     /gene="sppA"
                     /locus_tag="PK829_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOU40665.1"
                     /translation="DSGGGSALASELIGRQIEQFAAHKPVVVYMGNVAASGGYYVAAP
                     AGHIMSQTLTTTGSIGVITAKFSTAGLYERLSVERASLERGQHAGLYRDSDPMTDQER
                     AIFQRTVHETYARFVDVVARGRQLAPEAVDAVGGGRVWTGRQARERGLVDSHGDFLDA
                     IKKAAELAALTAGDIYAVSAFNIYPRTSQYTVYPNGSARLAEEAARLLSGEQLRALAG
                     RPLMLLPYELRFG"
     gene            complement(671..1234)
                     /locus_tag="PK829_05315"
     CDS             complement(671..1234)
                     /locus_tag="PK829_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40666.1"
                     /translation="MTKSDQVVRVRPLQPSDAPYLVDLFENMSSDSRYHRFLQTLEYI
                     DMESMWTQAELIAYNSAANARGLIAFCDTAERPDVPVAAVRYVRLNGYEAEIGVSVRD
                     DMQNKGIGSHLLGLLVEEAHNEGLSRLLAYVQNSNIAIWHILNRLNYPLSRRPDGGAT
                     EVVIHVGDVDGCEDTAIDYSPEPQLIG"
     gene            1486..4140
                     /locus_tag="PK829_05320"
     CDS             1486..4140
                     /locus_tag="PK829_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOU40667.1"
                     /translation="MSKNDTDSLRASLTELPFISPAQVEQWVELQDTIDHTRDYLIRA
                     VPQAQFSIDEAQKILAQRTYTLVFFGGTGVGKSTLINALLGRNLLPTGAVTAVTGTIV
                     YIEQADEGEDESLVLNYWSRDEFAERVRRLCQLGEIDSFDITNEGERTQARKDIEALE
                     ESRKDQAKTERDEYLDILLDCIDSYDNNKELFKAGTPPPMSLMLENEESLKHLREDGF
                     KGSQQRQIRLIKSATFRIHPKAGMPSLLMNGYLRIVDVPGLGAGMRLHEAITLEEMKR
                     EDAMIVLVTDAGRQRVDEMKSLSAVNWIKENRLFGLSGGDLDEAASKIFLVVNGGNVR
                     QAFDRLNSGLPEAELEVKEVTRYIAPNYWERYGSRGHNRPYFLVMAPSALYLQDPEHA
                     PQEFASETERIAKVFKDQLGNVDTKDPLNPDTKEALLKLSEVPLLREALIHFIQHERV
                     RSQLREAATRTRNALQALRFYYEKQLAARGVQPPFATSWEQLQERRYENVLARQQKEL
                     PRAFHNALLELSSRTNSDERFRQMLRPTLNGIKGMVQDAVQREVEMLLENYGTEYWDG
                     RDVTYDNLIWGTSGIEVPIKRILFQVELVMQDAVSKFMPEAADIMTAELDRTLEAHEI
                     LSRLERASYGQTYNYIIPGEADNAVPVADAFRILIGRIGRSFRYTCQQATMYELMKTN
                     RSVHARLSVGEQELALPTNERLLDVALYGPERVAREIAASMPQVAAAGNNNQAPAPTE
                     TEAPIEINILDSAPNGETVPDFDISFLGEKPAAAAPADLEPSYADLLDNVAVKVNRIF
                     ASIIDDLFSDDDLLPRLRRLFWLEAIKSERDFNNLLVKPMLRQHDRNLHKPELRKAIE
                     VDLESVSDMEELMRVWDGLHKLETGLAI"
     gene            4208..5419
                     /locus_tag="PK829_05325"
     CDS             4208..5419
                     /locus_tag="PK829_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40668.1"
                     /translation="MEDAALSLIRAFPDRPQLAAFAARLLDWLNSDLNPRGEPLSESR
                     ARALLLAAEALDEPTRHSFSQLTDTEPGARIALYGLLRESNLADNGDVAALAAAAAGA
                     PPPDRPAAAAWLALAVAAHAWRSGFPLDRLDPAAPPDPYSPAGQVIKRAAFFARQQVQ
                     RSATERDKLSRKLAYTGPGGPATLNGLRPDGTAAPLPPYYRPPVPVRYPEYTSGVQVN
                     PDELEQPAPPAPAAPLSISVDDLEPPAAPAAPLTQPPLRIDASQLEPPARPIPQPPAP
                     RPPSNVVMPTAAVSPARPAAPRRHKRGRAPMATTKLRVVVQAHPDGPGLYGLQVRVSS
                     RGIRRYVAGTTNKDGEFLCELPVQADAGLTYDIDVTWPRDFGGEVERKSITVNADRTH
                     FVLPFYRTLKA"
     gene            5434..6078
                     /locus_tag="PK829_05330"
     CDS             5434..6078
                     /locus_tag="PK829_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOU40669.1"
                     /translation="MNDSDRLRLTAEDIAAQDGRVTAPPVLTIGLADIPDAPPPAGQR
                     RHGIQITSADIAAFEPASEEVIGPLEQRAFALVNAARAEQLPRWLNRGPLRWHSALAA
                     VARQHSADMLQRRYVAHVTPEGISVSRRLERASISYLACGENIGVVYGPASRGEQGLI
                     EIQTAFMNQPRRLANHRGNILNPVWTHVGIGVAYSPEGQLIVTQNFVATLTNPA"
     gene            6120..>7084
                     /locus_tag="PK829_05335"
     CDS             6120..>7084
                     /locus_tag="PK829_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOU40670.1"
                     /translation="MTDTDTETIVITDPDSDRYHTFGYISWWQQEVVRNATVLVIGAG
                     ALGNEVIKNLTLMGIGNILIADFDTIEDSNLSRSVLFRADDRGRRKVDAAADGVKALN
                     PDVKVKAWHGDINFEMGLGVFRHVDAIVGCLDNREARLSINRFSWAVNRPWVDGAIQE
                     LMGIVRVFRPGEGACYECTLTDSDYQIINLRYSCPLLARQNILQGKVPTTPTSASIVA
                     AFQTQEALKLLHHMEVQPGKALMINGLTNDIYMTEYPVKDGCMSHSMLEPIVELPDVT
                     AAGTTLGALLAIARERLGPEAVLEFDSELVVAMECSSCGQDEPIF"
BASE COUNT         1316 a         2415 c         2205 g         1148 t
ORIGIN      
        1 cgattccggt ggcggctcgg cgctggcctc ggagttaatc ggccggcaga ttgagcaatt
       61 tgccgcccat aagccggtcg tcgtctacat gggcaatgtc gccgcctccg gcggctacta
      121 tgtggccgcg cccgccggcc acatcatgag ccagacgctg acgaccaccg gctccatcgg
      181 cgtgatcacg gccaagttca gcaccgccgg cctctatgag cggttgtcgg tcgagcgcgc
      241 cagcctggag cggggccaac acgccgggct gtaccgcgac agcgacccga tgacggacca
      301 ggagcgggcc atcttccagc gcacggtcca cgagacgtat gcgcgcttcg tcgatgtcgt
      361 cgcccgcgga cggcaactgg cccccgaggc ggtcgatgcc gttggcggcg gccgcgtctg
      421 gacggggcgg caggcccgcg agcgcggcct ggtcgatagt cacggcgatt tcctcgacgc
      481 gatcaaaaag gcggccgagt tggccgccct gaccgctggc gatatttatg ccgtctccgc
      541 cttcaatatc tacccgcgta cgtcgcagta tacagtttat cccaatggct cggcccgttt
      601 ggcggaggaa gctgcccgcc tcttgtcggg cgagcagctg cgcgcgctgg cgggccggcc
      661 gctgatgctc ctaccctatg agttgcggtt cgggtgagta gtcgatggcc gtgtcctcgc
      721 agccgtcgac atcacctacg tggatgacga cctcggtcgc cccgccgtcc gggcggcggc
      781 tgagcggata gttaagccga ttcaggatgt gccagatggc aatgttgctg ttctggacat
      841 aggcaagcag ccggctgagg ccctcgttgt gggcttcctc aactagcaag cccaacaggt
      901 ggctgccgat gcccttgttc tgcatgtcgt cgcgcacgga cacgccgatc tcagcctcgt
      961 atccattgag gcgcacgtag cgaaccgcgg ctacgggcac gtccggccgc tcggctgtgt
     1021 cgcaaaaggc gatcaggccg cgcgcattgg ccgcagagtt ataggcgatc aactctgctt
     1081 gcgtccacat gctctccata tcgatgtact ctagcgtctg taagaaccgg tgatagcggc
     1141 tatcgctact catgttttcg aacaaatcaa ccagataggg agcatcgctc ggctgcaagg
     1201 ggcggacgcg cacaacctga tcgctcttcg tcaaaaagtt gtatactcta gggtgattca
     1261 gcataatgat tctcgatgac gcattgcgtc atacacagaa tagcacaagc cggccataag
     1321 agcaagccga tccacgcttt cctacgcgat ttttacatcc acggccacga cgtcagctgc
     1381 aacccttaag gggtcattgc gtactgatga gtaacaacta cgtgattgca gtgcgcctgc
     1441 gtctgcgcgg gcggtgacga atcccggcga ggtaaggaac gacacatgag taagaacgat
     1501 accgattctt tgagggcttc actgacggaa ctgccgttca tcagcccggc gcaggtggag
     1561 cagtgggtcg aactgcaaga cacgatcgac cacacacgcg actacctcat tcgcgccgta
     1621 ccccaggctc aatttagcat cgacgaggcc cagaaaatcc tggctcagcg cacctatacg
     1681 ttggtcttct tcggcggcac gggcgtgggc aagagcacgc tgatcaacgc cctgctcggc
     1741 cgcaacctgc tgccaacggg cgcggtgacg gccgtgaccg gcaccatcgt ctacattgag
     1801 caggccgacg agggcgagga cgaatcgctg gtgctcaact actggagccg cgacgaattc
     1861 gccgagcgcg tccgtcgttt gtgccagctg ggcgagatcg acagctttga catcaccaac
     1921 gagggcgagc gcacccaggc ccgcaaggac attgaggcgc tcgaagagtc gcgcaaagac
     1981 caggccaaga ccgagcgcga cgaatacctc gacatcctgc tcgactgcat cgactcctac
     2041 gacaacaaca aggagttgtt caaggccggc acgcccccgc ccatgagtct gatgctggaa
     2101 aatgaggagt cgctcaagca tctgcgtgag gacggcttca agggcagcca gcagcgtcag
     2161 attcgcctga tcaagtcggc caccttccgc atccatccta aggccggcat gcccagcctg
     2221 ctgatgaatg gctatctgcg catcgttgac gtgcccggtc tgggcgcggg catgcgctta
     2281 catgaggcca tcaccctcga agagatgaag cgcgaggacg ccatgatcgt cctggtgacc
     2341 gacgccggcc gccagcgcgt cgacgagatg aagtcgctct cggccgtcaa ctggatcaag
     2401 gaaaaccgcc tctttggcct gagcggtggc gacctggacg aggccgcgtc gaagatcttc
     2461 ctcgtcgtca acggcggcaa tgtgcgccag gcgttcgacc ggctgaactc cggtctgccg
     2521 gaggctgagc tagaggtcaa ggaagtcact cgctacatcg cccccaacta ctgggagcgc
     2581 tacggcagcc gcggccacaa ccggccctac ttcctggtga tggccccatc cgccctctac
     2641 ctgcaagacc cggagcacgc gccccaggaa ttcgccagcg agaccgagcg cattgccaag
     2701 gtctttaagg atcagttggg caacgtcgat acgaaagacc cgctgaaccc ggacacgaaa
     2761 gaggctctgc tgaagctgag cgaagtgccc ctgctgcgcg aggccctcat ccacttcatc
     2821 cagcacgagc gcgtccgcag ccagttgcgc gaggcggcca cgcgcacccg caacgccctg
     2881 caagccctgc gtttctacta tgagaagcaa ctggccgcgc gtggcgttca gccgccgttt
     2941 gccaccagtt gggagcagct acaggagcgg cgctatgaga acgttctcgc ccgccagcag
     3001 aaggaactgc cccgcgcctt ccacaacgcc ctgctggagt tgagcagccg caccaacagc
     3061 gatgagcgct tccgccagat gctccggccg acgctcaacg gtatcaaggg catggtccaa
     3121 gatgccgtcc agcgcgaagt ggagatgctg ctggagaact acggcaccga gtactgggac
     3181 ggccgcgacg tgacctacga caacctcatc tggggcacca gcggcatcga agtgcccatc
     3241 aagcgcatcc tgttccaggt cgaactggta atgcaggacg ccgtctccaa gttcatgccc
     3301 gaagcggccg acatcatgac cgccgaacta gaccgcaccc tggaggcgca cgagatcctc
     3361 tcccgcctgg agcgcgccag ctatggccag acctacaact acatcatccc cggcgaggcc
     3421 gacaatgctg tgcccgtggc cgatgcgttt cgcatcctga tcggccgcat tggccgcagc
     3481 ttccgctaca cctgccagca ggcgacgatg tacgaactga tgaagacgaa tcgctcggtt
     3541 cacgcgcgcc tgagcgtggg cgagcaggag ttggctctgc cgaccaacga gcgtctgctc
     3601 gacgtggccc tctatggccc ggagcgtgtc gcccgcgaga tcgccgcctc gatgccacag
     3661 gtggccgcgg ccggcaacaa caaccaggcc cccgccccga ccgagacgga ggcgccgatc
     3721 gaaatcaaca tcctcgacag cgcccccaac ggcgagactg tgcccgactt cgacatcagc
     3781 ttcctgggcg agaagccggc cgccgcggct ccggccgacc tggaaccgag ctatgccgac
     3841 ctgctggaca acgtcgccgt caaggtcaac cgcatcttcg cctcgatcat cgacgacctc
     3901 ttcagcgatg acgatctgct gccgcgtctg cgccggctgt tctggctgga ggccatcaag
     3961 tccgagcgtg atttcaacaa cctcctggtc aagccgatgc tgcgccagca cgaccgcaac
     4021 ctccacaagc cggagttgcg caaggctatc gaggtcgatc tggagtccgt ctcggacatg
     4081 gaagagctga tgcgcgtttg ggacgggctg cacaagctgg agaccggcct ggcgatctag
     4141 tactggatga cagatgatgg ggtgggctgt ccgtgacgac ggctcgcccc attgacagga
     4201 gcatgtcatg gaagatgctg ccctcagcct gatccgcgcc tttcccgacc ggccgcagtt
     4261 ggccgccttc gcggcgcggc tgctcgactg gctcaacagt gacctcaacc cgcgcggcga
     4321 gccgctgagc gagtcgcgcg cgcgggcgct gctgttggct gccgaggcgc tcgacgaacc
     4381 cacccgccac agtttcagcc agttgaccga caccgaaccg ggcgcgcgca tcgcccttta
     4441 cggcctgttg cgcgagagca acctggccga caacggcgac gtggccgctc tggccgcggc
     4501 cgcggccggc gcgccgccac ccgaccggcc cgcggcggcg gcgtggctgg cgctggcggt
     4561 ggccgcccat gcctggcgca gcggcttccc gctcgaccgc ctggacccgg ctgcgccgcc
     4621 cgacccctat agccccgccg ggcaggtcat caagcgggcg gctttctttg cccgccagca
     4681 ggtgcagcgc agcgccacgg agcgcgacaa gctgagccgg aagctggcct acaccggccc
     4741 cggcggcccg gccacgctca acgggctgcg ccccgacggc acggccgcgc cgttgccgcc
     4801 ctactaccgg ccgcccgtgc ccgtgcgcta tcccgaatac acgtccggcg tccaggtgaa
     4861 ccccgacgag ttggagcagc ccgcgccgcc agccccggcc gcgccgctga gcatcagcgt
     4921 tgacgatctg gagccgccgg ccgccccggc cgcgccgttg actcagccgc cgctgcgcat
     4981 cgacgcctcc cagctggagc cgccggcgcg cccgatcccc cagccgcccg cgccgcgccc
     5041 gcccagcaac gtggtcatgc ccacagccgc agtcagcccg gcccgcccgg ccgcgccgcg
     5101 ccgccacaag cgcggccgcg cgcccatggc cacgaccaag ctgcgcgtcg tcgtccaggc
     5161 ccacccggac ggccctggtc tctatggcct gcaagtgcgc gtcagctcgc ggggcatccg
     5221 ccgctacgtg gccggcacaa ccaacaagga cggcgagttc ctgtgcgagc tgcccgtgca
     5281 ggccgacgcg ggactgacct atgacattga tgtcacctgg ccgcgcgact tcggtggtga
     5341 ggtggagcgc aagtccatca ccgtcaacgc cgaccggacg cacttcgttc tgccgttcta
     5401 ccggacactg aaggcctgag cgcgggcaag cccatgaatg attcggaccg gctgcggctg
     5461 acggccgagg acatcgccgc ccaagatggg cgcgtcacgg ccccgccggt gctgaccatc
     5521 ggcctggccg acattcccga cgcgccgccg ccggcaggcc agcgccgcca cggcatccag
     5581 atcacctccg ccgacatcgc cgctttcgag ccggccagcg aggaggtcat cggcccgctc
     5641 gagcaacggg ccttcgctct ggtcaatgcc gcgcgcgccg agcagctacc acggtggctc
     5701 aaccgtggcc cgctgcgctg gcattccgcg ctggcggctg tggcccggca gcactcggcc
     5761 gacatgctcc agcggcgcta cgtggcccat gtgacgccag aggggatcag cgtctcccgc
     5821 cggctggagc gggccagcat cagctacctg gcctgtggcg aaaacattgg tgtggtctat
     5881 ggcccggcca gccgtggcga gcagggtctc attgagatac aaaccgcgtt catgaatcag
     5941 ccacgccggc tggccaacca ccggggcaat atcctgaacc ccgtctggac tcacgtcggc
     6001 atcggcgtgg cctattcgcc cgaaggccaa ctcatcgtga cgcagaattt cgtcgccacg
     6061 ctgaccaatc cagcctgagg gcgacacaat ggcgctaaac gccgattgca ggaaagataa
     6121 tgacggacac ggacacagag actattgtca tcaccgaccc cgatagcgac cgctaccaca
     6181 ctttcggcta cattagctgg tggcagcaag aggtcgtgcg caacgcgacg gtgctggtca
     6241 tcggcgcggg cgcgctgggc aacgaggtga tcaagaacct gaccctgatg ggcatcggca
     6301 acattcttat tgccgacttc gacaccatcg aggacagcaa tctcagccgc tcggtgctct
     6361 ttagagccga cgaccgcggc cgccgcaagg tcgatgccgc cgccgacggc gttaaggcgc
     6421 tcaatcccga cgtgaaggtc aaagcctggc atggcgacat caacttcgag atgggcctgg
     6481 gcgtcttccg ccatgtggat gctatcgtcg gctgcctcga caaccgcgag gcgcgcctgt
     6541 cgatcaatcg cttctcctgg gcggtcaacc ggccgtgggt cgatggggcc atccaggaac
     6601 tgatgggcat cgttcgcgtc ttccggccgg gcgagggggc gtgctacgag tgcacgctga
     6661 ccgacagcga ctatcagatc atcaacctgc gctactcctg ccccctgctg gcccggcaga
     6721 acatcttgca gggcaaggtg ccaacgacgc cgaccagcgc gtctattgtg gccgccttcc
     6781 agacgcagga ggcgctgaag ctgctgcacc acatggaagt gcagccgggc aaggcgctga
     6841 tgatcaacgg cctgaccaat gacatctaca tgaccgagta cccggttaag gatggctgca
     6901 tgagccactc tatgctggag ccgattgtcg aactgcccga cgtgaccgcc gctggcacca
     6961 ccctgggggc gctgctggcc attgcccgcg agcgcctggg gccagaggcg gtgctggagt
     7021 tcgacagcga gctggtggtg gctatggagt gcagctcctg cggccaggac gaacctatct
     7081 tcca
//
LOCUS       DAOSMQ010000115         7081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10656_length_7081_cov_4.448335, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000115 DAOSMQ010000000
VERSION     DAOSMQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10656_length_7081_cov_4.448335"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="PK829_05340"
     CDS             <1..1768
                     /locus_tag="PK829_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017160303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOU40671.1"
                     /translation="HEDLAWRSLPVNERLTHALVKGINAYIETDVEEARQTVERPLHV
                     IEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAYLVPYIEAEKARSGDRGPVG
                     RILLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMTPTHKILETARRENVDIIGLSG
                     LITPSLEEMRHVAQEMEREGFDIPLLIGGATTSKVHTAVKIDPEYHRGPVIHVHDASR
                     AVGVASQLLSKEHHAGFAAGVRAEYAAIRQSRGDRGGGKQLASLAEARANRLALDWSA
                     YTPPAPNKPGVTVLDVDLAELCATIDWTPFFTAWELHGKFPAILNDPVVGESARPLYA
                     DAQHMLNCLIEEQWLRARAVVGLFPANSVGDDIVVYPAGERGSGGAGATAALSTVHCL
                     RQQMQRPPGRPNLSLADYIAPAGSGLTDTLGFFAVTAGLGLDEVVRRYEAAHDDYNAI
                     LAKALADRLAESLAEWTHALVRRELWGYAPDEALDNDALIAEQYRGIRPAPGYPSCPD
                     HTEKGELFRVLDATNAAGIHLTESFAMLPAASVSGYYFAHPQAAYFGMGRIGRDQLED
                     YAARKGMSVELAERWLASSLGY"
     gene            1947..2258
                     /locus_tag="PK829_05345"
     CDS             1947..2258
                     /locus_tag="PK829_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40672.1"
                     /translation="MLFLAACGSEGMAVAPELIDPDAITARAFGAAECFVTLPEAVPA
                     RFNPDPEQVPAGEIPAGEVEAVQVVTLRDDGGLWYQVTTDGMWVRVDTVDYTTRGDCR
                     P"
     gene            complement(2255..3469)
                     /locus_tag="PK829_05350"
     CDS             complement(2255..3469)
                     /locus_tag="PK829_05350"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40673.1"
                     /translation="MAAEPPAEPVDLWLTENGTLLTTALSAAQALRLRQQYGEHLVVQ
                     PGWEPGSFVLTARQTIGVLVLDGLRIHIAPKVPLTNLFHMLTYAYDLPFFREEEAPLA
                     VGDDLFEFLVAIFVQQVNRLARQGIQRGYRDVEEDASYLRGRLLLGEQLQRNAGRPAR
                     FAQRVNDFTADRLENRILKATLWQLAGAPYRDADLRRRVRRTLSAFSEVSLVAVRPEE
                     CRQVVYNRLNARYRMPINLAQLFLRHLSLEGRTGETPFMSYLLPMAGVFERFVARYLD
                     DHFAAHPLISVLTQESIWLDEALRERGRLDILLHWAGQPAMIVDTKYKTFDGTPSEAD
                     RNQMFMYCHALGVTRAMLVYADEGAAGYEARFPGMWLAARSLALDGALADFRRRCQDF
                     AQEVMARVTAGP"
     gene            complement(3459..5009)
                     /locus_tag="PK829_05355"
     CDS             complement(3459..5009)
                     /locus_tag="PK829_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU40674.1"
                     /translation="MPWRADFRGGAADRTATGPQDKRLPNDDALAPPFDALFGDRATA
                     DWALELFAETVKRLGGWPDDPRFALTLPRGRKMLRLNLGNWMVMDVSASGVLHLTALI
                     APMAEAFSFPRGEPFARTEDEIAVFAIPLATARAWPEELQRVYSESMLALAERFGSYG
                     GSPHRHAHQAELFRALFNVAEREHLLLAGLPEGKGENGSRRSQPPPDRPAVVREAAPR
                     WSVAEPPVVAVYGRDDFLRETYLTTPAADELHDLLLERQQIVLYGPPGTGKTHVARHL
                     GRWLIGLADPPPERLTVIQFHPAYSYEEFIEGIRPESHGRGEHARVDYLARPGVFVRF
                     CRAAESIAGPCVFVIDEINRGNIARVFGELMLLLEYRDEAVPLPYSGDRFRIPPNVYL
                     IGTMNTADRSIALVDFALRRRFHFFRFDADPELFDRWLAKQPSPLPYLSALYRRLATE
                     AVDDPDYAIGPSIFMRHLDEAGLARVWRRSVMPYLEEYYIDQRARARRWAWDGELVRG
                     LREQPDGG"
     gene            complement(5213..5938)
                     /locus_tag="PK829_05360"
     CDS             complement(5213..5938)
                     /locus_tag="PK829_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOU40675.1"
                     /translation="MNDNDLRPDALFDLSNPTTAAFFDGCAYVWEALAQLERHVARLT
                     AGGRRIDGTVMDGAWLGDAPIVIEAGALVEPGAYIVGPAYIGRGAVVRHGAYVRENVI
                     LLDGAVLGHASEAKHALFLPNAHAPHFAYVGDSILGQGVNLGAGTKLSNLGILSAKDP
                     LTGKRPTIKLTIDDREYDTGLTKFGAILGDGVQTGCNAVLNPGCIVGPGTLVYANTSL
                     RKGYHPAGSIIKLRQNVRVMTRE"
     gene            6155..6472
                     /locus_tag="PK829_05365"
     CDS             6155..6472
                     /locus_tag="PK829_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOU40676.1"
                     /translation="MQFIETPIFTRHIMELLSDEEYSRLQEVLLMRPEAGDLIPGGGG
                     LRKLRWRVSGTGKRGGLRTIYYWDVSSEVCYMLFVYKKSAKSDLTQQQLRILRNLVKE
                     YLQ"
     gene            6469..6768
                     /gene="nadS"
                     /locus_tag="PK829_05370"
     CDS             6469..6768
                     /gene="nadS"
                     /locus_tag="PK829_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HOU40677.1"
                     /translation="MNNEEFAELVESVKQAGEIKRGLRQPSRVLEFSPMDIKGIRAKL
                     DKSQHEFALMIGVSLGTLQNWEQGRRKPVGPARALLRVAARSPEAFRDALETPLP"
BASE COUNT         1273 a         2262 c         2357 g         1189 t
ORIGIN      
        1 tcacgaagac ctggcctggc ggtcgctgcc ggtcaatgag cggctgaccc acgcgctggt
       61 gaagggcatc aacgcctaca tcgagacgga cgtggaagag gctcggcaga cggtcgagcg
      121 cccgctgcac gtcatcgagg ggccgctgat ggacggcatg aacgtcgtcg gcgacctgtt
      181 tggcgcgggc aagatgtttc tgccgcaggt ggtcaagagc gcccgcgtca tgaagcaggc
      241 cgtggcctat ctcgtgccct acatcgaggc ggagaaggcc cgcagcggcg accgcggccc
      301 ggtcgggcgc attctgctgg ccacggtcaa gggcgacgtg catgacatcg gcaagaatat
      361 cgtcggtgtc gtcctgggct gcaacaacta cgaggtcatc gatctgggtg tcatgacccc
      421 tacgcacaag attctggaga cagcccgccg cgagaacgtg gacatcatcg gcctcagcgg
      481 cctcatcacc ccgtcgctgg aggagatgcg ccacgtggcc caggagatgg agcgcgaggg
      541 cttcgacatc cccctgctca tcggcggggc gacgacgtcg aaagttcaca cggccgtcaa
      601 gatcgatccg gagtaccatc gcgggccggt catccacgtc cacgatgcct cgcgggccgt
      661 cggtgtggcc tcgcaattgc tcagcaagga gcaccacgcc ggttttgccg ccggcgtgcg
      721 cgctgagtac gccgccatcc gccagagccg cggcgaccgc ggcggcggca agcagttggc
      781 gtcgctggcc gaggcccgcg ccaaccggct ggccctcgac tggagcgcct atacgccgcc
      841 cgcgcccaac aagccgggcg tgactgtcct cgatgtcgat ctggccgagc tatgcgcgac
      901 catcgactgg acgcccttct tcaccgcctg ggagctgcac ggcaagttcc ccgctatcct
      961 caacgacccg gttgtgggcg aatcggcccg ccccctctac gccgacgccc agcacatgct
     1021 gaactgcctc attgaggagc agtggctgcg ggcgcgggcg gtggtagggc tgttcccggc
     1081 caactccgtg ggagacgaca ttgtcgttta cccggcgggg gagcggggga gcgggggagc
     1141 cggggcgaca gcggccctga gcacggtcca ctgcctgcgc cagcagatgc agcgtccgcc
     1201 gggccggccc aatctctctc tggccgacta cattgccccg gccggcagcg gcctgaccga
     1261 caccctcggc ttcttcgccg tcaccgccgg gctggggcta gacgaagtcg tgcgccgcta
     1321 tgaggctgcc cacgacgact acaacgccat cctggccaag gccctggccg accggctggc
     1381 cgaatcgctg gccgagtgga cgcacgcgct ggtgcggcgc gagttatggg gctatgcgcc
     1441 cgacgaggcg ctggacaacg atgccctcat cgccgagcag taccgcggca tccgtcccgc
     1501 gccgggctac ccctcttgcc ccgaccacac tgagaagggc gagctattcc gcgtgctcga
     1561 cgccaccaac gccgccggca tccacctgac ggagagcttc gccatgctgc cggcggcctc
     1621 ggtcagcggc tactacttcg cccacccgca ggcggcctac tttggcatgg gccgcatcgg
     1681 ccgcgaccaa ctggaggatt acgccgcccg caagggcatg agcgtcgaat tggccgagcg
     1741 ctggttggcg tcgtcgctgg ggtactgaac cagcgacgcg cggtgaactc gcccggcagg
     1801 cgagttcacc gcgcgtgtct agcgctgcgt ggcgtgcagg atagtgaagc cgacgatctc
     1861 gttagtctga acacacgatc cctgatctga gaagccctat gtgccatcat cgccgcctta
     1921 cacgtctgac gatatgtctt tcgctcttgc tcttcctcgc cgcctgtggc agcgagggca
     1981 tggccgtcgc cccggaactg atcgaccctg acgccatcac cgcgcgcgcc tttggcgcgg
     2041 cggagtgctt cgtcaccctg cccgaagccg tgcccgcccg cttcaacccc gacccggagc
     2101 aagttccggc cggcgaaatc ccggccggtg aagtggaagc tgtccaggtc gtgaccctgc
     2161 gcgacgacgg tgggctgtgg tatcaggtga ccaccgacgg catgtgggtg cgcgtcgata
     2221 cggtcgatta caccacccgc ggcgattgcc ggccctaagg gccggcggtc acgcgcgcca
     2281 tcacctcctg ggcaaagtcc tggcagcgcc ggcgaaagtc ggccaatgcc ccatccaacg
     2341 ccagcgatcg cgccgccagc cacatgccgg gaaaccgggc ctcatagccg gccgcgcctt
     2401 cgtcggcgta gaccagcatc gcccgcgtca ccccaagcgc gtggcagtac atgaacatct
     2461 ggttgcggtc ggcctccgag ggcgtgccgt cgaacgtctt gtacttggtg tccacgatca
     2521 ttgccggctg acccgcccaa tgcagcaaga tgtcgagacg gcccctttcg cgtaacgctt
     2581 cgtctagcca gatcgattcc tgggtcagga cgctgatcag ggggtgggcg gcgaaatggt
     2641 cgtccaggta gcgggcgaca aagcgctcaa acaccccggc cattggcagc aagtagctca
     2701 taaagggcgt ctcgccggtg cgcccctcca gcgacaggtg gcgcaggaac aattgcgcca
     2761 ggttgatcgg catgcggtag cgcgcgttca ggcggttata gacgacctgc cggcactcct
     2821 ctggccggac ggcgaccaac gacacctccg agaaagccga cagtgttcgc cgcacgcgcc
     2881 ggcgcaagtc ggcgtcgcga tagggcgcgc cggctagctg ccacagcgtg gccttcagga
     2941 tgcggttctc cagccggtcg gcggtaaagt cgttgacgcg ctgggcgaaa cgcgccggcc
     3001 ggccggcgtt gcgctgcaac tgctcgccca gcagcagccg gccgcgcaag taggacgcat
     3061 cttcctccac atctctgtag ccgcgctgga tgccctggcg ggccagccgg ttgacctgct
     3121 gcacgaagat ggccaccaga aactcgaaca ggtcgtcgcc cacggccaac ggcgcttcct
     3181 cctcgcggaa aaagggcagg tcataggcgt aggtcagcat gtggaacaga ttcgtcagcg
     3241 gcactttggg ggcgatgtgg atgcgcaggc cgtcgaggac gagcacgccg atggtctgcc
     3301 gggccgtcag gacaaacgac cccggctccc aaccgggctg aacgacgaga tgctcgccgt
     3361 actgctgccg cagccgcagc gcctgcgccg cgctcagcgc ggtggtcagc agcgtgccgt
     3421 tctcggtcag ccacagatcg accggttcgg ccggcggctc agccgccatc aggctgctcg
     3481 cgcaggccgc gcaccagctc gccgtcccat gcccaccgcc gggcgcgggc gcgctggtcg
     3541 atgtagtact cttccagata gggcatgacg ctgcgccgcc acacacgggc cagccccgct
     3601 tcgtcgagat ggcgcatgaa aatgctgggg ccgatggcat agtcagggtc gtcgaccgct
     3661 tcggtcgcca ggcggcgata gagcgcgctg aggtagggca gcggcgatgg ctgcttggcc
     3721 agccagcggt cgaacagctc cgggtcggcg tcaaagcgga agaaatggaa gcggcggcgc
     3781 agggcgaaat cgaccagggc aatggagcgg tcggccgtgt tcatcgtgcc gatcagatag
     3841 acgttgggcg ggatgcgaaa gcggtcgccg gagtagggca gcggcacagc ctcgtcgcgg
     3901 tactccagca gcagcatcaa ctcgccgaag acgcgggcga tgttgccgcg attgatctcg
     3961 tcgatgacga agacgcacgg cccggcaatg ctctcggcgg cccgacagaa gcggacgaag
     4021 acgcccgggc gggccaggta gtccacgcga gcgtgctcgc cgcgcccgtg gctctccggc
     4081 cggatgccct cgatgaactc ctcgtagctg taggcggggt ggaactggat gacggtcaga
     4141 cgctccggcg gcgggtcggc cagcccaatc agccagcgac ccaggtggcg ggcgacgtgg
     4201 gtcttgcccg tccccggcgg gccatagagg acgatctgtt ggcgctccag cagtagatcg
     4261 tggagttcgt cggctgccgg cgtggttaag taggtctcac gcaggaagtc gtcgcggcca
     4321 tagaccgcca ccaccggcgg ctcagccaca gaccaacgcg gcgcggcttc gcgcaccaca
     4381 gcggggcggt ccgggggcgg ctgcgaccgc cggctcccat tctcgccttt cccctccggc
     4441 aagccggcga gcagcaaatg ttctcgttcg gccacgttga aaagcgcccg aaacagctcg
     4501 gcctgatggg cgtgccggtg gggcgagcca ccgtagctgc cgaagcgctc agccagggcc
     4561 agcatgctct ccgaatagac gcgctgcaac tcttctggcc acgctctggc cgtcgccagc
     4621 ggaatggcga agacggctat ctcatcctcg gtgcgcgcaa acggctcgcc acgcggaaac
     4681 gagaaggctt cggccatcgg cgcgatgagc gcggtcagat ggagaacgcc gctggcagaa
     4741 acgtccatca ccatccagtt gcccaggttg agccgcagca tcttccggcc gcggggcaac
     4801 gtcagggcga agcgcgggtc atcgggccag ccgccgaggc gcttaaccgt ctcggcgaag
     4861 agttcgagcg cccagtcggc cgtcgcccga tcgccaaaga gggcatcgaa aggaggagct
     4921 aaagcgtcgt cgttgggcaa gcgcttgtcc tgcgggccgg ttgccgtccg gtcggccgcg
     4981 ccgccgcgga agtcggcgcg ccacggcaag ctcggctcac agcaacaagt ataggcaggt
     5041 cgttgcacaa tgggcaagtt tgggaggaag ccgaatagga cgccgattca cgcacaggag
     5101 acactcgcgg atcgacgtga atctgggcca tccgtgtcat ccgcgtgcgg ttcccccgcc
     5161 cttggccaca gcccgcgcgg ttatgctaca ctcgcccacc atgcgagact tattattccc
     5221 ttgtcatcac gcgcacattt tgccgcaact tgatgatgct gccggccggg tggtaaccct
     5281 tgcgcagact ggtgttggcg tagacgagcg tgcccgggcc gacgatgcag ccagggttga
     5341 ggacggcgtt gcagcccgtc tgcacgccgt cgcccaggat ggccccgaac ttcgtcaggc
     5401 cggtgtcgta ttcgcggtcg tcgatggtca gcttgatcgt cggccgcttg cccgtgagcg
     5461 ggtctttcgc gctcagaatg cccaggttgc tcagcttcgt acccgcgccc aggttgacgc
     5521 cctggccgag gatggagtcg ccgacgtagg cgaagtgggg cgcgtgggcg ttgggcagga
     5581 agagggcgtg cttggcctcg ctggcgtggc cgagcaccgc cccgtcgagc aggatgacgt
     5641 tctcgcgcac gtaggccccg tggcgcacga ctgcgccgcg gccgatgtag gccgggccga
     5701 cgatgtaggc ccccggctct accagcgccc ccgcctcgat aacgatgggc gcgtcaccca
     5761 gccacgcccc gtccatcacc gtgccgtcga tgcgccggcc gccggccgtc agccgggcta
     5821 cgtgccgctc cagttgggcc agcgcctccc agacgtaggc gcagccgtcg aaaaaggccg
     5881 ccgtagtggg gttggaaagg tcgaatagcg cgtcggggcg caggtcgttg tcgttcatag
     5941 gggttctcct ctgtaaacgg attttagcgg aacaagcggg aaaggcattg tggatcacgc
     6001 gcccaacggc aatcgcacga tcgcttctaa gtgtcgtggc tggggacgtt gtgggaaagc
     6061 aagattaagg cgggttgcga aggcgacttc tcgaaactcg cctggacgga gtaattgggg
     6121 ttgacaatag tacgccattg acgcatagta ccgtatgcag ttcatcgaga cgcccatctt
     6181 cacaaggcac ataatggagc tgctatcaga tgaggagtac agccgccttc aggaagtgtt
     6241 gctaatgcgg cccgaggccg gcgacctgat acccggcggg ggtggattgc gcaaactgcg
     6301 ctggcgagtg tccggcacgg gaaagcgagg cggtctacgc actatttact attgggatgt
     6361 ttcttcggaa gtttgctaca tgctatttgt ttataagaaa agtgctaaga gcgatctaac
     6421 acagcagcag ttgcgaattc tacgcaacct tgttaaggag tacctacaat gaacaatgag
     6481 gaattcgcag aattggtcga gagtgttaag caagctggtg agatcaagcg cggcctgcgt
     6541 cagccaagcc gagttttgga gttctcgccg atggacatta aaggcatccg cgcgaaactg
     6601 gacaagtcac agcatgagtt cgcgttgatg atcggcgtca gcctgggaac cttgcagaac
     6661 tgggagcagg gacgccgcaa gcctgttggg ccggcgcgag cactgcttcg cgtagctgct
     6721 cgcagtcccg aggcatttcg tgatgcattg gaaacaccgt tgccctgacg gaaaaggtct
     6781 aataaggaag gctttatgat ggcctatgat ctttgcgcgg ttgagtggct gcctgataga
     6841 agatccttat tcagcgacaa gcgctcggtc agggtcacag ggtttaatgc cgctggtgaa
     6901 cttcacattc tatacgtggg tgaggaaaag tataatgcgc gatcggcacg tgagttaaaa
     6961 gctctcggac tgtaaggtta gccctcttcc ttctttatcc tgatcacccc ctcccgcgta
     7021 atctgatagt ggtctagatc ccgcgccaca tgggtattgg cgaaagtggc gcgggcctcg
     7081 g
//
LOCUS       DAOSMQ010000116         7023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10769_length_7023_cov_5.614811, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000116 DAOSMQ010000000
VERSION     DAOSMQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10769_length_7023_cov_5.614811"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PK829_05375"
     CDS             <1..715
                     /locus_tag="PK829_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40678.1"
                     /translation="GFPRKADGFVYTADTFVDAWRKRQGFHQPDAVDYDALIDEFDMI
                     AKVNSGAVDEFWLFAFPYAGYYESIMGGPGAFWCNAPPLTQTAHAARRFVIMGFNYQR
                     GVGEMLENFGHRAESIMKHVFREKRDDDNLWERFFRHEFKNPGQAEVGWMHYAPNSER
                     DYDWGNKRRVPSRWHTWQNFPDLRGEAQMVDCNDWGNGDIRLHHKWWFNLLPHINGAA
                     DGIAYNWWKYIVDPNTVTW"
     gene            731..1189
                     /locus_tag="PK829_05380"
     CDS             731..1189
                     /locus_tag="PK829_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40679.1"
                     /translation="MNNSAVRWLIIIALALVLGLLLWWLLGARDVGAPGETDTPGDTT
                     QCPQATPEFFTVEPVTSPTDALTQIITVDLGNGEAITVTTESGVFTVPFDAFPKEIEI
                     TLLPNTTHNLTVEGKVRQVQQGDCTYGGYTLTTTTDRNGAPLVIEQNSGQ"
     gene            complement(1266..4139)
                     /locus_tag="PK829_05385"
     CDS             complement(1266..4139)
                     /locus_tag="PK829_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU40680.1"
                     /translation="MTLRLYFLGPPRIERDGQTIEPDTRKATALLAYLAVTGQFQTRD
                     ALAALLWPDMDDQHARAALRRTLSSLRAFAGDAALHVSRDGLGVNPDGLWCDVTAFTS
                     AVVETGRHAHSHAPDKPCPDCIARLEAAVALYRDAFLSGFSLRDSAAFDDWQVVAGEG
                     LRRSLTTALAWLVAAHSAAGAFDRAADHARRWLAVDPLREEAHRWLMRLYAWGGARDL
                     ALRQYREAVRVLDEELGVEPLPETTALYEAIQENRLERPAPVVAAPPSQAAAPPAAVP
                     APAAAPLLGRDTAWAELQRAYKDVTAAGRLVAITGEAGIGKTRLAEAFLEWARAGGAT
                     TVTATCYEGEADLAYAPFVAALNALLRRPESAARLPAIPAQWLAEAARLAPELAGGAA
                     PPADSGPGAQTRFFAGISEVICHLLSGPVPGVFCLDDAHWADVASLDLLAYLARRLSD
                     LPVLLVVAWSDAAGGAPRLRRLLTEAQRTATGRAVVLTRWRPSDVLGLVAAGAEPSAD
                     TAAVAGRLYRETEGLPFFVVEYLNALRANPDSWAMPQSVRDLLAGRLAGAGQTERQLL
                     QAAAVIGGAFDDDILLAASGRSEEEVVGGLERLLEHGLIREAAEAGGVAGDMGYDFSH
                     QQLRSLVYDETTLARRRLLHRRVAAALTARARGEAMASLIGHHYRLGGQEALAADAYR
                     RAGDHARGLFANREALGHYQTALALGHPATAALYEASGDLYTLLGEYSRGLSAYESAA
                     ALAGPADLARLEHKLGQLYERRGQWELADSHYAEALSRYQPATAGADIARLCVDRSRV
                     AYHRGDSAGARALAEEALALAAEDAEARAQAHNTLGILARGAGDREAARRHLEESLAL
                     AERAEATAVRVAALNNLARLRGAAGEIDAALELLELAIDLCARQGDRHREAALLNQRA
                     DWLHRAGREDEAMSSLKAAVAIYAEIGQESGGWQPEIWKLTEW"
     gene            4268..4735
                     /locus_tag="PK829_05390"
     CDS             4268..4735
                     /locus_tag="PK829_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40681.1"
                     /translation="MADDASWTTQQKKTIGDVAVDGLLAGMTAGLVMAGVLLVLRLLD
                     GAGVAETLGRFDPGSNNSPIVGGLMHLAVAGLDGALFALAYYTLRRRLPVLGHYSWLA
                     GAAYGLVLWLVAQSVLMRELNAGLAVIAPWQFALAHLAFGIVLGYAIGRHAGE"
     gene            4744..6192
                     /locus_tag="PK829_05395"
     CDS             4744..6192
                     /locus_tag="PK829_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40682.1"
                     /translation="MSATNGTQLGRRLNRPAALTLTFVLAFLALDVAQVVYRFFLPTD
                     GWVTTEVALDSPQALYYQNVVGRPSGLRPMDIVTGMAGQPLESFAQGVRPDFWQAGAV
                     VEYTVERAGQELRLPVPIDHWTPSALLTGFITLVPQAVSDLGSFLVLGISLLAFLRRP
                     AEPAAWALLVFAAALSAPSISRLIPLDYSIGFDPYTSILTIFYSYMVYGIVIAPALLA
                     FTLVFPRPKPLVVRHPWVAYAPFGIGVAVAIVLFSGGSVYFGYVLTMLMLLASLAGLA
                     HSALTMRDAVSRAQLRWAIGGLAAGIGLFLLKYPDAFDWLPDQVAYWFAVVSSLGVPV
                     MGIGLAVAVLRYRLFDVDVIIRRTTSYAILTALLAMVYFSAVVLLEHFLTPLIGDSTP
                     SVVLSTLLIAALFLPLRRRVQSAIDRRFFRRKYDAEKTLAAFAATVRDETDLDALLAE
                     LTRAIQETMQPEHVSVWLRPVDGGRPVANDGP"
     gene            complement(6624..>7023)
                     /locus_tag="PK829_05400"
     CDS             complement(6624..>7023)
                     /locus_tag="PK829_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006618696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOU40683.1"
                     /translation="DTNILSEPLRPQPNEAVLRLLRQHQAELATATLVWHELLFGCYR
                     LPPSARRAAIEQYLFAVVHPSLLLLAYDEAAAQWHAAERARLSGLGRTPAFVDGQIAA
                     IAATQQLTLVTHNYGDYQMFDGLTVVDWFE"
BASE COUNT         1103 a         2363 c         2348 g         1209 t
ORIGIN      
        1 cggcttcccg cgtaaggccg acggctttgt ctacacggcc gacaccttcg tggacgcctg
       61 gcgcaagcgc cagggctttc accagccgga cgcagttgat tacgacgcgc tcatcgacga
      121 gttcgacatg atcgccaagg tcaactccgg cgcggtcgat gagttctggc tgttcgcctt
      181 cccctatgcc ggctactacg agtcaatcat gggcgggccg ggcgcgttct ggtgcaacgc
      241 gccgcccctg acgcagaccg cccacgccgc ccgccgcttc gtcatcatgg gcttcaacta
      301 ccagcgcggc gtcggcgaga tgctggagaa cttcggccac cgcgccgagt cgatcatgaa
      361 gcacgtcttt cgcgagaagc gcgacgacga caacctgtgg gagcgcttct ttcgccatga
      421 gtttaagaac cccggccagg ccgaagttgg ctggatgcac tacgcgccca atagcgagcg
      481 cgactacgat tggggcaaca agcgccgcgt gcccagccgc tggcacacgt ggcagaactt
      541 ccccgatttg cgcggcgaag cccaaatggt ggactgcaat gattggggca acggcgacat
      601 ccggctgcat cacaagtggt ggttcaacct gctgccccac atcaacggcg cggccgacgg
      661 catcgcctac aactggtgga agtacatcgt tgatcccaac acggtcacct ggtagaaggg
      721 gaggagtgac atgaacaatt ccgcagtgcg ctggctgatc atcatcgccc tggccctggt
      781 gttggggctg ctgttgtggt ggctgctggg ggcgcgcgac gtgggcgcgc cgggcgagac
      841 cgacacaccc ggcgatacga cccaatgtcc ccaggccacg ccggagttct tcaccgtcga
      901 gccggtgacc tcgccgaccg acgccctgac acagatcatc accgtcgatc tgggcaatgg
      961 cgaggccatc actgtcacga ccgagtcagg cgtcttcacc gtccccttcg acgccttccc
     1021 caaggagatc gagatcacgc tgctgcccaa cacgacccac aatctgaccg tcgagggcaa
     1081 agtacgccaa gtccagcagg gcgattgcac ctatggcggc tacacgctga cgacgaccac
     1141 cgaccgcaac ggcgcgccgc tggtcatcga gcagaacagt ggccagtagg gcagtgggcc
     1201 agtagggcag tgatcagaca tcaggatgac ttgaagctgg ctgatctact gaccactgct
     1261 cccccctacc actccgtcag cttccagatc tccggctgcc agccgcccga ctcctggcca
     1321 atctcggcgt agatggccac ggccgccttc aggctgctca tagcctcgtc ctcgcgcccg
     1381 gcgcggtgga gccagtcggc gcgctggttc agcagggccg cctcgcggtg gcggtcgccc
     1441 tggcgcgcgc acaggtcgat ggccaattcc agcagttcca acgcggcgtc gatctccccg
     1501 gccgcgccgc gcaggcgggc caggttgttg agcgcggcca cgcgcacggc cgtcgcctcg
     1561 gcgcgttcgg ccaacgccag gctctcctcc agatgacggc gagccgcctc ccggtcgccc
     1621 gccccccgcg ccaggatgcc cagcgtgttg tgggcctgcg cccgggcttc agcgtcctcg
     1681 gcggccagcg ccagcgcctc ctcggccagc gctcgcgccc cggcgctgtc gccgcgatgg
     1741 taggcgacgc ggctgcgatc gacgcacagg cgggcgatgt cggcgcccgc tgttgccggc
     1801 tggtagcgac tcagggcctc ggcatagtgg ctgtcggcca gttcccactg ccctcgccgc
     1861 tcgtagagct ggcccagttt gtgctccagc cgggccaggt cagccgggcc ggccagggcc
     1921 gcggccgact cgtaggcgct caggccgcgg ctgtactcgc ccaatagcgt gtagaggtcg
     1981 ccactggcct cgtacagcgc cgccgtggcc gggtggccca gggccagggc ggtctggtag
     2041 tggcccagcg cctcgcggtt ggcgaagagg ccgcgggcgt ggtcgccggc gcggcggtag
     2101 gcgtcggccg ccagcgcctc ctgcccgccc agccggtagt ggtggccgat caggctggcc
     2161 attgcctcgc cgcgcgcccg cgccgtcaga gccgcggcca cgcgccggtg cagcaaacgc
     2221 cgccgggcca gcgtcgtctc atcgtagacc aacgagcgca attgctggtg gctgaagtcg
     2281 tagcccatat cgccggccac accgccggct tcagccgcct cgcggatcag cccatgctcc
     2341 agcagccgct ccagcccacc aacgacctct tcctcgctgc gaccgctggc cgccaggagg
     2401 atgtcgtcgt cgaatgcccc gccgatgacc gccgccgcct gcaagagttg gcgctcagtc
     2461 tggcccgcgc cggccagccg cccggccagc aggtcgcgca ccgactgggg catagcccag
     2521 ctatcggggt tggcccgcaa ggcgttcagg tactcgacga caaagaaggg cagtccctcc
     2581 gtctcgcggt agagccggcc ggcgacggcg gccgtgtcgg cgctcggttc cgccccggcg
     2641 gccacaaggc caagcacatc tgacggccgc cagcgggtca ggacgacggc gcggcccgtt
     2701 gctgtgcgct gcgcttcggt cagcaggcgg cgcaggcgcg gcgcgccgcc ggcggcgtcg
     2761 ctccaggcca caaccagcag cacgggcagg tcgctcagcc gccgcgccag gtaggccagc
     2821 aggtcgagcg aggccacgtc ggcccagtgg gcgtcgtcga ggcagaagac gccgggcacg
     2881 gggccagaca gcaagtgaca gatgacctcg ctgatgccgg cgaagaagcg cgtctgcgcg
     2941 ccggggccgc tgtcggccgg cggggcagcg ccgccggcca gctcgggggc cagacgagcg
     3001 gcctcggcca gccactgcgc cggaatggcc ggcaggcgcg ccgccgattc gggccgccgc
     3061 aagagggcat tcagcgcggc gacaaatggc gcgtaggcga gatccgcctc gccctcgtag
     3121 caggtggccg tcaccgtcgt tgccccgccg gcgcgcgccc actccaggaa cgcctcggcc
     3181 aggcgcgtct tgccgatgcc cgcctcgccg gtaatggcca ctagccggcc ggccgcggtc
     3241 acatctttgt aggcacgttg cagttccgcc caggcagtgt cgcgaccaag cagaggcgcg
     3301 gcggccggcg cgggcacagc cgcgggcggc gcagcggctt gtgatggcgg cgcggcgacg
     3361 accggcgcgg gccgttccag acggttctcc tggatggcct cgtacagcgc cgtcgtctcc
     3421 ggcagcggtt ccacgcccaa ctcctcatca aggacgcgca ccgcctcgcg gtactgccgc
     3481 agggccaggt cgcgcgcccc gccccaggcg tacagacgca tcagccagcg gtgggcctcc
     3541 tcgcgcagcg gatcgaccgc cagccagcgg cgggcgtggt cggctgcccg gtcgaacgcg
     3601 ccggcggcgc tgtgggcggc caccagccag gccagcgccg tcgtcagcga gcggcgcagg
     3661 ccctcgccgg caaccacctg ccagtcgtca aacgcggcgc tgtcgcgcag cgagaagccg
     3721 ctcagaaagg cgtcgcggta gagggcgacg gccgcctcca gccgggcaat gcagtcaggg
     3781 cagggcttgt cgggggcgtg gctgtgggcg tggcggcccg tttcgaccac ggcggacgtg
     3841 aacgcggtca cgtcgcacca cagaccatcg gggttcacgc ccaacccatc gcggctgaca
     3901 tggagggccg cgtcgccggc gaaggcgcgc agcgacgaca gcgtgcggcg cagcgccgcg
     3961 cgggcgtgct ggtcgtccat gtccggccag agcagtgcgg ccaacgcgtc gcgcgtctgg
     4021 aactggcccg tcacggccag gtaggccagc agcgccgtgg ccttgcgcgt gtccggctca
     4081 atcgtctgcc cgtcgcgttc gatgcgcggc gggccaagga agtagaggcg cagcgtcatc
     4141 gtgagcgcga ttatgctgga agcgtctcca agttacaagg cgaggcgatt tcgtaacgat
     4201 tcccgtaacg atcaggagac gctcgacggc tacactgcgt ctgaaatgga acgtaggaga
     4261 gttcaggatg gccgatgacg ccagttggac aacgcagcag aaaaagacga tcggcgatgt
     4321 ggccgtggac ggtctgttgg cggggatgac cgcgggactc gtcatggcag gcgtgttact
     4381 cgtgctgagg ttgttggacg gcgcgggtgt ggcggaaacc ctcggccgct tcgatccggg
     4441 tagcaacaac tcgcccatcg tcggcggcct gatgcacctg gcggttgccg gactagatgg
     4501 ggcgttgttt gcgctggcat actacacgct gcgccggcgg ttgccggttc tgggccacta
     4561 cagctggtta gccggcgctg cctatgggct agtgctctgg ctggtggcgc agtctgtctt
     4621 gatgagagag ctgaacgccg gcctggccgt gatcgcgccg tggcagttcg ccctggccca
     4681 tctcgccttt ggcatcgtcc tagggtatgc tattggtcgc cacgcgggcg aataggtgat
     4741 tgcatgagcg caacgaatgg gacacagcta gggcgccgcc tgaaccggcc cgccgcccta
     4801 acgctaacct tcgtcctcgc gtttctggcg ttggacgtgg cacaggtcgt ctatcgcttc
     4861 ttcctgccaa ctgacggctg ggtaaccacc gaggttgccc tggactcgcc gcaggccctc
     4921 tactatcaaa acgtggtcgg acggccatct ggtttgcgac ccatggacat cgtcaccggc
     4981 atggcgggcc aaccgttgga atcgtttgcc cagggcgttc gcccggactt ctggcaggct
     5041 ggtgcggtcg ttgaatacac ggtggaacgg gctggccagg agttgcgcct gcccgttccg
     5101 atcgaccact ggacgccatc ggcgctgctg acgggtttca ttaccctcgt tccacaggcc
     5161 gtcagcgacc tgggctcgtt ccttgttctg ggcatttcct tactggcctt tctccgaagg
     5221 ccggcggagc cggcggcctg ggccttgctg gtctttgcag ccgcgctgag cgcgccgagt
     5281 atcagtcgcc tgattccgct cgattactca atcggttttg acccttacac cagcatcctc
     5341 accattttct acagctatat ggtctatggg atcgttattg cgccggcgct gctcgcgttc
     5401 acgctcgtct tcccacggcc gaaacccctg gtggttcgcc atccctgggt agcctatgcg
     5461 ccgtttggca tcggggtagc cgtggcaata gtgctcttct ccggcggcag cgtctacttc
     5521 ggctacgtat taacgatgct gatgctgctc gcatccctgg ccggcctcgc gcacagcgcc
     5581 ctgacgatgc gtgatgccgt cagccgcgcg cagttgagat gggccattgg cggtttagcg
     5641 gcgggcattg ggcttttcct gcttaagtat cccgacgcct tcgattggtt gccggatcag
     5701 gttgcttact ggtttgcggt agtgtcgagc ctgggagtgc cggtcatggg cattggtctg
     5761 gctgtcgccg tcctgcgcta ccgactgttc gacgtggacg tcatcattcg acggacgacc
     5821 tcttatgcga tcctgacagc gctgttggcg atggtgtatt tcagcgcggt ggtcctcctg
     5881 gagcactttt tgacgccgct catcggtgat tcgacgccgt ccgtcgtcct gtctaccctg
     5941 ctgatcgctg ctctctttct gccgctgcgc cgccgcgtgc aaagcgctat cgaccggcgc
     6001 ttcttccgcc gcaaatacga cgcggagaaa acgctggcgg cgttcgccgc cacagttcgc
     6061 gatgagacag acctggacgc gctgctggct gaactcacgc gcgccatcca ggaaaccatg
     6121 cagcccgagc acgtgtcggt gtggttgcgg ccggtggatg gtggacgacc ggtggccaat
     6181 gatgggccat agcctgacga tcgcatcccc tgcttttata gagtcagtgg cgccggcgga
     6241 atcaagcggc tacggggttt atactcggca atccttcccg ctgcgcgaca gtgatgagtt
     6301 cgtcggcggc gatcacgacg aactgctgct gaaaatccgt cgttagcttt tgaatcacac
     6361 gcgccgcact tcctggccga aatgcgcctc ccttggcccg gcgggccaga gcggctatgc
     6421 cttcgacaat ggcgagtcac gtttgactgt ttcggcaatc ggttcgccgc tgatagcagc
     6481 cggcgcgtcg cccgcggctt gatctcggca acgaggccgg cgctcaataa tctggtttgc
     6541 caggcagcgc tgctatccag tgacttgcca aatacgctga ggacggccgc caggggcacg
     6601 tctgtccggc atcaggtgtt ttgctattca aaccaatcga caacggtcag gccgtcgaac
     6661 atctggtagt cgccgtagtt gtgcgtgacc aacgtcagtt gttgcgtggc ggcaatggcc
     6721 gcgatctgac catcgacaaa tgcaggggtg cgccccagcc cgctcagccg cgcccgctcc
     6781 gccgcgtgcc actgggcggc ggcttcatcg taggccagca acaggaggga ggggtggaca
     6841 acggcgaaca ggtactgctc aatcgccgcc cgccttgccg acggcggcaa acggtagcag
     6901 ccgaaaagca actcgtgcca gacgagtgtg gcggtagcca attcggcctg gtgctggcgc
     6961 aacagccgca ataccgcttc gttgggctgt ggtcgcagcg gttccgagag aatattggtg
     7021 tcc
//
LOCUS       DAOSMQ010000117         6940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10967_length_6940_cov_4.728105, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000117 DAOSMQ010000000
VERSION     DAOSMQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10967_length_6940_cov_4.728105"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK829_05405"
     CDS             complement(<1..204)
                     /locus_tag="PK829_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOU40684.1"
                     /translation="MLELRLETAEADAALPSALPLDEAVAAATPTPGGGSVAAQTGAL
                     AAALAQMAAGLTVGRKRYLDVSEE"
     gene            complement(187..1068)
                     /gene="ftcD"
                     /locus_tag="PK829_05410"
                     /pseudo
     CDS             complement(187..1068)
                     /gene="ftcD"
                     /locus_tag="PK829_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
     gene            complement(1118..2839)
                     /locus_tag="PK829_05415"
     CDS             complement(1118..2839)
                     /locus_tag="PK829_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOU40685.1"
                     /translation="MSRRRPRVIRNKGVIAAARVQRTPPPGARLPFQVDPWTRRLVRP
                     LLIAGVATAPALGMLVIVSIAQPDQPWLTLVWLCFFAALEGAYTAAWLHHPDSRAVDR
                     GTYRVAEVFLLLVLARIVSWVVFGVGLPSPDEMRVFLGAPLSFVMVGNFLTTAFVTLF
                     AWYVAVVMGRLFAQLDVGVEEVQFYTLSPAERQAQADNRPISTPRGVLQTQYMNRWIG
                     LGMLMVFLAALSTFEVGQFASVANVPDIARLGLRPAMLFALLLYFLGGLWLLSHARLL
                     RLNAQWLADGFGKEADLERSWQRSALLVVLLIAFAAAFLPIGSTLGISRILQLLLNIL
                     FYIAGLIFAAIGFLFASFLSLFVRNAEQLPPVTPEPVPTFAPTPVPTPPAQTNPAFAF
                     VFTSIFWAILIALAIGALLFFLRERGYRLDLSRVQHSVSALGQQARALWRRLRRRAKA
                     LSHNLRERLRSPDDAAPTAGPPPLGLGRRRGLSPREQVRYYYLAAVRRAGQSGVPRAD
                     NATPLEYARELKQHWPEAESELEQLTDAFLEARYSPRPIDHPMVARVKAEWKRLRERL
                     RRAAPRP"
     gene            complement(2836..4128)
                     /locus_tag="PK829_05420"
     CDS             complement(2836..4128)
                     /locus_tag="PK829_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU40686.1"
                     /translation="MPPYLWLALLLLAIAFLLRVDFVFYILYVLAGVFLWSRWHAARA
                     LTHLVASRAYRRRAFLGEQVEVTVTLENKSRLALPWVQFQESVPPELRLEESTQHVVS
                     LAGRGQRSHVYHVKGLQRGYYRLGPLRLTSGDLFGLAKSRVGYLPSDFLTVYPRIVPV
                     SRLGLPSRLPFGAIASQQRLFEDPARPMGTRDFRSGDPLRQINWKASAHTQKLLVRTF
                     QPAISLETMILLDLHASDYERRDRLYWTEWAIVTAASIATHLVNQRQAVGLMSNGVDP
                     LRIQEDAREFDEVTGRLLFQTLVGESARSYLPTPIPPGDGRSHLMKVLEQLARLDTRD
                     TIPLSEWASTAGVRLSWGVTILAITGQGDEPRCNALHRLARAGFNPILIAVEPDANFG
                     LVRERARRLGFRAYNLTSRADLDRIARPEVGRPAGATP"
     gene            complement(4128..5081)
                     /locus_tag="PK829_05425"
     CDS             complement(4128..5081)
                     /locus_tag="PK829_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOU40687.1"
                     /translation="MATNEIQEFSRRVIDNVERVIIGKRGALELIMVAILCEGHILLE
                     DVPGTGKTMLARALAQSLGIDFKRLQCTPDLLPNDVTGVSVYQPQSQTFAFIPGPAFT
                     NILLADEINRATPRTQSALLEAMGERQVTVDGITRPLERPFFVIATQNPIEYEGTFPL
                     PEAQLDRFFLKLTLGYLDERFESQMLVNLGRAHPIDTLQPVVDGRRIVELSRQVWDVH
                     VDDTVRDYIVGLVFGTRRHSDLLLGASPRGSHTLYRAAQTYAALQGRDYVLPDDVKRL
                     AAPVLAHRCLVHPESALRGVAVADVIGDILARTPLAIEESG"
     gene            5244..>6940
                     /locus_tag="PK829_05430"
     CDS             5244..>6940
                     /locus_tag="PK829_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40688.1"
                     /translation="MSVHRPPPPIDTTFNVDFADRLARYETYNKHHYRPNTYLHKWWG
                     RRCGSTFRLILKGLVDDPARADYYAAGGLEGKIILDPMIGGGTTLHEAIRLGAGVIGL
                     DIDPIPVLQARATLTEGRLDSLRAGFNDFYTRLRDESGDCFQTHCPVCDEPTPSWYTL
                     YGARRRCSCGEALVVDSLIIRQQPDGKVVRLCPHCGDLDGGTGHVCAPAGVPLVEKGQ
                     GACPTCGTAYGDLDELPFYARYRMLVVAGHCPVHGLFHRAPDWRDHERVASADARRAM
                     LPFAPEAFGVVSGDKSAQLLRRGIHSYLDLFSSRQLLFLDAAGRCLPDEPSVRLNLAL
                     LVSTALEFNSMLCGYKGMEQRRAGAVRHTFAYHGYAFPYTALETNPLYPRQASGTLLK
                     LYAGRIERARRWAARPQERSLDTARATFVPIPGERDSGQEVSCAAELAGGPQRFFVRQ
                     ASAVTLPVESGSVDAVVTDPPYYDSIQYDDLAAFFRVWLRHFLPDAADWGYDGAGAAI
                     HSERVPGDSRYVQMMTAIFRECRRALRPEVGRLIFTFHHWQPQAWAALTTALYDAGFV
                     "
BASE COUNT         1158 a         2244 c         2452 g         1086 t
ORIGIN      
        1 ttcctcgctc acgtccagat aacgcttgcg gccgacggtc agcccggcgg ccatctgcgc
       61 cagcgccgcg gccagcgcgc ctgtctgcgc ggccacggag ccgccgcccg gcgtgggcgt
      121 cgccgcggcc acagcctcgt ccaggggaag cgcgcttgga agagccgcgt cggcctcggc
      181 ggtttcaagc cgcagctcta acacctggtc gtcgcggaac ggctcgatct gcaagtacca
      241 gcgcgcggcg tcgagcagtg ccttttgcgg aatcagcccg accagttcgg cgcgggtgat
      301 cgtcaggcca tgctgagctg cctcgcgccg caccatctct tgcacgcgat agatgggcgt
      361 gcgctcgaag ttagtcaggt tcatgctgac ctgggcttgc ccctcgacga ggaaaccgcg
      421 cgcctggaca tggcgcagac cgccgttgct gaagcggatg cccctggcga tctgctcggc
      481 cacggcggcg tcatcggcgt taaggtagag gttgtaggcg atcaggaacg gccgcgcgcc
      541 aatgaccgtc gccccccacg ggcgcggctc agccggccca aagtcgggca gacggcgcgg
      601 gttggtggcc acctcctgct tccatagctc gtactcgcct ttgcgaatat cgctcaggcc
      661 ctcgcgctcc ggccgcgtgg ccgccgcgcc atacagatag accgccacgc ccagctcctc
      721 gccgacgcgc ttgcccagtt gccgcgccag ggccacacag ccctcggtgc tgatgccgcg
      781 gacgggcacg aaggggcaga cgtcggtcgc gccgatacgc gggtgctcac cctggtggtg
      841 gtcgaggtcg atcaggtcgc gggcggtctg gatggcgcgg aaggcggcct cgacaacctc
      901 gtctggcccg ccgacaaagg tgatgaccgt gcggttgtgg tcggcgtcgg cgctgacgtc
      961 gagcacctgg acgccggcga cagcgcggat ggcgtcggcg atctggccgt agacagccgg
     1021 gtcgcggccg ttgctgaagt taggcacaca ttcgacaagg ggttgcatga caattctcct
     1081 gatcgcaagg ggttatagcg gtaaagtggc cggtgggcta cggacgcggc gcagccctac
     1141 gcaagcgctc acgaaggcgc ttccactcgg ccttgacgcg ggcgaccatc gggtggtcga
     1201 tggggcgagg gctgtagcgg gcctccagaa aggcatccgt cagttgttcc aactcggact
     1261 ccgcttccgg ccaatgctgc ttcagctcgc gggcatactc caggggcgtc gcgttgtcgg
     1321 cccggggcac gccactctgc ccggcgcgcc ggacggcggc caggtagtag tagcgaacct
     1381 gctcgcgggg ggacaggcct cgccggcggc cgagaccgag cgggggcggg ccggcggttg
     1441 gcgcggcgtc gtcggggctt cgcagccgct cgcgcaggtt gtggctcagc gctttggccc
     1501 ggcggcgcag tcgtcgccac aaagcgcggg cctgctggcc caacgcgctc accgagtgct
     1561 gtacccggct gaggtctagc cggtagccgc gctcgcgcag gaaaaagagc agcgcgccga
     1621 tcgccagagc gatgagtatt gcccagaaga tggaggtaaa gacaaaggcg aaggccgggt
     1681 tcgtttgcgc cgggggcgtt ggtacgggcg tcggcgcgaa cgttggcacc ggctctggtg
     1741 tgactggtgg cagttgctcg gcgttgcgga cgaacaaact gaggaagctg gcaaagagga
     1801 agccgatggc ggcgaagatc agtccggcga tgtagaagag gatgttgagc agtagttgga
     1861 ggatgcggct gatgcccagc gtcgagccga tgggcagaaa ggccgcggca aaggcgatga
     1921 gcagcacaac cagcagggcg ctgcgctgcc aggagcgctc caaatcggcc tccttgccga
     1981 aaccatcggc cagccattgg gcgtttaggc gcaacaggcg ggcgtggctc agcagccata
     2041 ggccaccgag gaagtagagc agcagggcaa agagcatggc cggccgcaga cccagccggg
     2101 caatgtcggg cacattggcg acggacgcga actggccgac ctcgaaggtg ctcagcgccg
     2161 ccagaaaaac catcagcatg ccgaggccga tccagcggtt catgtactgt gtttgcagga
     2221 cgccgcgcgg cgtggagatg ggccggttgt cggcctgggc ctgccgttcg gctggtgata
     2281 gcgtgtagaa ctgcacttcc tctacgccca cgtccagttg ggcgaaaagg cggcccatca
     2341 ccacggccac ataccaggcg aacagggtca caaaggcggt ggtcaggaag ttgccgacca
     2401 tgacgaagct gagcggcgcg cccagaaaga cgcgcatttc gtctggcgag ggcaggccga
     2461 cgccgaagac cacccagcta acaatgcggg ccaggacgag cagcaagaag acttccgcca
     2521 cgcggtaggt gccgcggtcc acggcgcggc tgtcggggtg gtgaagccag gcggcggtgt
     2581 aggctccctc cagcgcggcg aagaagcaca gccagaccag cgtcaaccac ggctggtccg
     2641 gctgggcaat actgacgatg accaacatgc ccagggccgg ggctgtggct accccggcga
     2701 tgagcagcgg gcgcaccagc cggcgcgtcc acggatcgac ctgaaagggc agacgcgcgc
     2761 ccggcggcgg cgtgcgctgg acacgggcgg cggcaatgac acccttgttg cgaatgacgc
     2821 gcggccgccg gcggctcatg gcgtcgcccc tgcgggccgc cccacctccg gccgcgcgat
     2881 acgatccagg tcggcccgac tggtcaggtt ataggcgcga aagcccagcc gccgcgcccg
     2941 ttcgcgcacc aggccaaagt tggcgtctgg ctcaacggcg atgaggatgg gattgaagcc
     3001 ggcacgggcc agccggtgca gggcgttgca gcgcggctcg tccccctggc cggtgatggc
     3061 caggatggtg acgccccaac tgaggcggac gccggccgtc gacgcccact cggacaacgg
     3121 aatggtgtcg cgcgtgtcga ggcgagcgag ctgctccagc accttcatca ggtgcgagcg
     3181 gccgtcgccc ggcggaatgg gggtgggcag gtagctgcgc gcgctttccc cgaccagggt
     3241 ctggaacagc aaccggccgg tcacctcgtc gaactcgcgc gcatcttcct ggattcgcag
     3301 cgggtccacg ccgttggaca tcagcccgac ggcctggcgc tggttgacga ggtgtgtggc
     3361 gatggaggcg gcggtgacaa tggcccactc cgtccagtag aggcggtcgc ggcgctcgta
     3421 gtccgacgcg tggaggtcaa gcaggatcat cgtctccagt gagatggccg gctggaaggt
     3481 gcgcacaagc agcttctggg tgtgggcgct ggccttccag ttgatctggc gcagcgggtc
     3541 gccggagcga aagtcgcgcg tccccatcgg ccgggccggg tcttcgaaca ggcgttgctg
     3601 gctggcgatg gcgccaaagg gcaggcgcga cggcagcccc agccgcgaga cggggacgat
     3661 gcgcggatag acggtcagga agtcggatgg cagatagccg acgcgcgact tggccaggcc
     3721 gaacaggtcg ccgcttgtca gccgtagcgg gccgaggcgg tagtagccgc gctggagacc
     3781 tttgacgtga tagacgtggc tacgctggcc gcggccggcc aggctgacga cgtgttgcgt
     3841 cgattcctcc agacgtagct ccggcggcac actctcctga aattgcaccc atggcagagc
     3901 caggcggctt ttgttttcca gcgtcaccgt cacctcgacc tgttcgccca ggaaggcacg
     3961 ccggcggtag gcgcggctgg ccaccaggtg ggtcagcgcg cgcgccgcgt gccagcggct
     4021 ccacaggaag acgccggcga gcacgtagag gatgtagaag acgaagtcga cgcgcagcag
     4081 gaaggcgatg gccagcagca acagggccaa ccataggtag ggaggcatta gccggattcc
     4141 tcgatggcca acggcgtccg ggccaggatg tcgccgatga cgtcggccac cgcgacgcca
     4201 cgcagggcgc tctccggatg caccaggcag cggtgggcca gaacgggcgc ggccaaacgc
     4261 ttaacgtcgt caggcaggac gtagtcgcgc ccttgcagcg cggcgtaggt ctgggcggca
     4321 cggtagagtg tgtggctacc gcgcgggctg gcccccagca ggaggtcgct gtggcggcgc
     4381 gtgccgaaga ccaggccgac gatgtagtcg cgcaccgtgt catcgacgtg gacgtcccag
     4441 acctgccgtg acagctcaac gatgcgccgg ccatcgacaa cgggctggag ggtgtcgatg
     4501 gggtgcgccc ggccgaggtt gacgagcatc tgactctcga agcgctcgtc caggtagccg
     4561 agggtcagct tcagaaagaa gcggtcgagc tgcgcctccg gcagcgggaa ggtgccctca
     4621 tactcaatgg gattctgcgt ggcaatcaca aagaaggggc gctccagcgg ccgggtaatg
     4681 ccatcgaccg tcacctggcg ctcacccatc gcctccagca aggccgactg cgtgcgcggc
     4741 gtggcccggt tgatctcgtc ggccagcagg atgttggtga aggccgggcc agggatgaag
     4801 gcgaaggtct gcgactgcgg ctggtagacc gagacgccgg tcacatcgtt gggcagcagg
     4861 tcgggcgtac actggaggcg cttgaagtcg atgcccaggc tttgggccag cgcgcgggca
     4921 agcatggtct tgcccgtgcc gggcacgtcc tccaacagga tatgtccctc gcacaggatg
     4981 gccaccatga ttagttccag cgcgccgcgc ttgccgatga tgacgcgctc gacattgtcg
     5041 atcacgcgtc gcgagaactc ctggatttcg tttgttgcca tacgcctcta ttgcgaacct
     5101 gcggccgggg acatggcggg atgttatcat agacaggcgc aatggggtaa gtggggttag
     5161 acttgaccgg tctctatgat tgcccggatc ggtcgggctg attctctggc cggctgtcgc
     5221 tacgccttgc tacaataagc ccaatgtctg tccatcgccc gccgcccccc atcgacacca
     5281 catttaacgt ggactttgcc gatcgcctgg cacgctacga gacctacaac aagcaccact
     5341 ataggcccaa tacctactta cacaagtggt gggggcggcg ctgcggcagc acgttccggc
     5401 tcatcctcaa ggggctggtc gatgatccgg cgcgagcgga ctactacgcg gccggtgggt
     5461 tagagggcaa aatcatcctc gaccccatga tcggcggcgg cacgacgctg cacgaggcca
     5521 ttcgcctggg cgcgggcgtc atcgggctgg acatcgaccc catccccgtc ttgcaggcgc
     5581 gggccaccct gaccgaaggg cgtctggatt cgctgcgggc cggcttcaac gacttctaca
     5641 cgcggttgcg cgacgagagc ggcgattgct tccagacgca ctgccccgtc tgtgacgaac
     5701 cgacgccgtc gtggtacacg ctctatggcg cgcgccgtcg ttgtagctgc ggcgaagcgc
     5761 tggtcgtcga ttcgctgatc atccggcagc agcccgacgg caaggtagtg cggctctgtc
     5821 cgcattgcgg cgacctggat ggcggcactg gccacgtctg cgccccggct ggcgttccgc
     5881 tggttgagaa ggggcagggg gcctgcccca cctgtgggac agcctacggc gacctcgacg
     5941 agttgccgtt ctacgcccgc taccggatgc tggtcgtcgc cggccactgc cccgtccacg
     6001 ggcttttcca ccgcgcgccc gactggcgcg accacgagcg agtggcgtcg gccgatgccc
     6061 gtcgggcgat gttgccgttc gcgccggaag cgtttggcgt ggtcagcggc gacaagtcgg
     6121 cccagctgct gcgccggggc attcacagct atctcgatct tttctccagc cgccagctgc
     6181 tcttcctcga cgcggccggc cgctgcctgc ccgatgagcc gtcggtgcgc ctcaacctgg
     6241 ccctgctggt gtccacggcg ctggagttca actcaatgct gtgcggctac aagggcatgg
     6301 agcagcgacg cgcgggggcg gtgcgccaca ccttcgctta ccacggctac gccttcccct
     6361 acacagcgct ggagacgaac ccgctctatc cgcgccaggc ctccggcacg ctgctgaagc
     6421 tctacgccgg gcgcatcgag cgcgcccgcc gctgggctgc gcggccccag gagcgcagcc
     6481 tggacacagc gcgggcgacg tttgtgccca tccccggcga gcgagacagc gggcaggagg
     6541 taagctgcgc cgccgagttg gccggcggac cgcagcgctt ctttgtccgc caggcgtcgg
     6601 ccgtcaccct accagtcgaa tcgggcagcg tcgacgccgt agtgaccgat ccgccctact
     6661 acgacagcat ccagtacgat gacctggccg cgtttttccg tgtctggctg cgccacttcc
     6721 tgcccgacgc ggccgactgg ggctatgacg gggcgggggc ggccatccac tcggagcgcg
     6781 tccctggcga cagccgctac gtgcagatga tgacggccat cttccgcgag tgccgccgcg
     6841 cgctgcggcc ggaggtcggc cggctcatct ttactttcca ccactggcag ccgcaggcgt
     6901 gggcggcgct gacgacggcg ctctatgacg ccggcttcgt
//
LOCUS       DAOSMQ010000118         6934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_10979_length_6934_cov_4.761593, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000118 DAOSMQ010000000
VERSION     DAOSMQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10979_length_6934_cov_4.761593"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK829_05435"
     CDS             complement(<1..304)
                     /locus_tag="PK829_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40689.1"
                     /translation="MKMRRISKKEMETIHPAIPDAYEKLEQGRISRREFIRFATLLGM
                     SAATATIAAACGGGGEAVATTVAPVAGASTAEATSAPADTSTGGPRRGGTIIKAMGL"
     gene            746..1225
                     /locus_tag="PK829_05440"
     CDS             746..1225
                     /locus_tag="PK829_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU40690.1"
                     /translation="MTHRAVLLEALASTPADIARLARGLDEAAAARRPAGGRSCRDLI
                     AQLSYSEEHSRAQFRRILSEDAPALTAPDLTLAVDAGEGMAVLVEQFARARAETLAWL
                     SALGPGAWQRPAVHPTGGRTTLRFLVGELVASDIALTNQLVTVRNQWRAARPEVTDE"
     gene            1222..1839
                     /locus_tag="PK829_05445"
     CDS             1222..1839
                     /locus_tag="PK829_05445"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOU40691.1"
                     /translation="MRTAFILCGALARETQAIIDRHGWAVDVYGIPAIDHMHPERIAP
                     DVARKLRAIRDGHDRVLVVFGDCGSRGALDDLLAREGLARIDGPHCYEMYGGLLFDEL
                     MAEEPGTFFLTDFLVRGFRGTIWHGLGLDRYPQLREDYFRHYRRLVFMTQSGQPELLD
                     KAQTIAAMLDLPLEVRHTGYGQLEARLVAWMASASLLEDDAPAAT"
     gene            complement(1849..3054)
                     /locus_tag="PK829_05450"
     CDS             complement(1849..3054)
                     /locus_tag="PK829_05450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU40692.1"
                     /translation="MSIEMRARGRYPLVLAFTCYVALGMMGGLLSIAWPSIRVTFGLP
                     LDALAALLAAATTGVVAGSFLSAQIMARLGVGWSLLLANAVGVLAAIGYVLAPTWIAM
                     IVAGLLWGLAQGVINTSLNIYVAANHSVRTMNWMHASFGVGATIGPLLMTAIVATGLS
                     WRLGYLAVAGVHLGLALLFITVAGTMNFRGANQATEDTRPVPMRATLRLLVVWLSILL
                     FMLYTGVETTAGQWTYTWFTEARGASAYVAGTITSVFWAMSTLGRIVFGAGAARIGIE
                     RLLRTCMVGVVLAALLLLPRTLPTGLAAIILMGLSLSIIFPTLTAHTPARVGRRHTAN
                     TISVQTGAASIGLALLPGLAGIIAQRVGLEVLGPFLVVGAALMLLLNTATVAVARRET
                     TAVVETPVN"
     gene            3185..4150
                     /locus_tag="PK829_05455"
     CDS             3185..4150
                     /locus_tag="PK829_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU40693.1"
                     /translation="MGLGLAALGRPGYINLGHATDLKREYDVAAMAEHAYAVLDVAWQ
                     AGIRYFDVARSYGRAEEFLAGWLRARHIAPGQVSAGSKWGYTYTADWQVLAERHEVKD
                     HSLPVLRRQIGESRALLGDYLSLYQIHSATLDSGVLENGEVLAELARLRDGGLVIGLS
                     LSGPNQAETLRRALAIEIGGAPLFRSVQATWNVLAQEAGPALAEAHNRGMGVIVKEAL
                     ANGRLTERNIDPAFAEQRRLLSAAAAETGVGLDALALAAVLVQPWADVVLSGAARAEH
                     LASNVAAVALATRRRAWLHDWLRHARTLAEPADVYWRTRAALAWN"
     gene            complement(4147..5844)
                     /locus_tag="PK829_05460"
     CDS             complement(4147..5844)
                     /locus_tag="PK829_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOU40694.1"
                     /translation="MAESLRQSLARYYREIRSSQARSIMVVLILIVILALAVLGVQFL
                     VRERGSLALLNWLNLLLIPLLLVTAAVWLAGTWKRTQLEIARLRDERALVDSYYDRLT
                     ELLLGRNLRGAAADDEVARAARAHTLMTLRQLNSEGRGHIVRFLYESSLLSAEQPAVD
                     LQRADLSGSDLSGVAMAGISLRNVDLHEARLVETALPASDLRGADLDRANLTRADLSE
                     SYLRGATLREAVLAGAQLRGAIMTKALLTDADLRDADLSEANLAGADLTRANLAGANL
                     KGARLNGANLTDATLDGANLSLANLNKATLSGVQATAADFSGSNLAGATLVHARLNGT
                     MFGGAILSEADLTDAELRDSNLNLANLRGAVMNQAIVTNANLRDAIMGRVSLSGSDLT
                     GSDLSRANLSIANLNRAILNRTNLEEAGLSGADLRAAQLKGAMLRGAILRGAILGDAD
                     LSRADLSKANLRWANLSNANLTGADLTEVDLTDAEVAPQILAKARAVTKVERSDSGQR
                     YDVVPTATAVREGPLPSTQVPTYQRPGAAPAAPDAGTSGTTAAPRQPDKSGPLPGQN"
     gene            complement(5856..6677)
                     /locus_tag="PK829_05465"
     CDS             complement(5856..6677)
                     /locus_tag="PK829_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOU40695.1"
                     /translation="MSDWVYQDELRLTELNVPRAALQFARTLAYPRLDVANYMTALHT
                     LSELATEHVDLAGPVARQADQLAEYLFVTLRFRGNTEDYGDPRNSYLNEVLDRRLGIP
                     ISLSVIFIDIAHRLGIPAYGIGLPGHFIVGIHSRDADIWLDPFHGGRRLDLTDCIDLV
                     RLAVGYGGALEDAWFAPLPPREILARMLNNLRSSYVRAAAWKEAARVIRLLRQTQPEV
                     AEHVRDLGLVYYQQRQMTQAAHYLDVYLQRSPAAADAQVIRDGMKELLNLWATQN"
     gene            6766..6897
                     /locus_tag="PK829_05470"
     CDS             6766..6897
                     /locus_tag="PK829_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOU40696.1"
                     /translation="MYIEVHLGDRVRLRKKHPCGNDEWDIIRLGADIGLVCCRCRRR"
BASE COUNT         1198 a         2219 c         2350 g         1167 t
ORIGIN      
        1 gcaagcccat ggccttgatg atcgtgccgc cgcgacgcgg gccgccagtg ctggtgtcgg
       61 ctggggcaga cgtggcctcg gccgtgctcg cgccggcaac cggcgcgact gtcgtcgcaa
      121 cggcttcgcc ccccccgccg caggccgcgg cgatggtcgc tgtggccgct gacatgccca
      181 ggagtgtggc gaagcggatg aactcgcgcc gcgaaatgcg cccttgctcc agcttctcgt
      241 aggcgtcggg gattgccggg tgaattgttt ccatctcctt cttggagatt cttcgcatct
      301 tcatgtgttc tcctccgaag atgacccgcc tttctggccg gtcacaagcg ttggtttgtc
      361 ccaattagca ttgctagtgc gcgtctgtct ccgcctcacc tccgtgttgc cggacggaca
      421 ggagcgccca ccggtaggaa taggggctag aagtgaagag cagtaggtga tgtgggccac
      481 gtcgtggggc aacggggcgc gagggccagt ctgaaacccg cccaggtcgg cgcgcggagg
      541 gaggggaggc ggctgtcagg ttcgtggtgt taagcccttt aacgactgat cctcgcaggg
      601 tgggcagcgg cccggcttgc cggtcacgcg cagcgaccct gagaaagtcg ccgatgaaat
      661 gctatccttc ggcggccaga tgatagcatg agttaggtga tgtgcaagcg ttgggcacgg
      721 ctggcggcta tactccacgg tcacaatgac ccaccgcgca gtgcttctag aggccctggc
      781 ctccaccccg gccgacattg cgcgcctggc gcgcgggcta gacgaggccg ccgctgcccg
      841 gcggccggca ggcggccggt cgtgccgcga tctcatcgcc cagctaagct acagcgagga
      901 gcacagccgc gcccagttcc ggcgcatcct gagtgaggat gcgccggctt taaccgcgcc
      961 agacctgacc ctggcggtcg atgctggcga gggaatggcc gttctggtgg agcaatttgc
     1021 ccgcgcccgc gccgagacgc tggcctggct gagcgcgctc ggccctggcg cgtggcagcg
     1081 gccggctgtc caccccaccg gcgggcgcac cacgctgcgc ttccttgtgg gcgagctagt
     1141 cgccagcgac atcgccctga ccaaccagct tgtcacggta agaaatcaat ggcgagccgc
     1201 gcggccggag gtcacggacg aatgaggacg gctttcattc tttgcggcgc gttggcgcgt
     1261 gagacgcagg ccatcatcga ccgccacggc tgggcggtcg atgtctatgg cattccggcc
     1321 attgaccaca tgcatccgga gcgcatcgcc cccgatgtgg cgcgcaagtt gcgcgccatc
     1381 cgcgacggtc acgaccgcgt cctggtcgtc ttcggcgatt gcggctcacg tggagcgctc
     1441 gacgacctgc tggcccgtga ggggctggcg cgcattgacg gcccccactg ctacgaaatg
     1501 tacggcgggc tgctgttcga tgagttgatg gccgaggaac caggcacgtt cttcctgacc
     1561 gatttcctgg tgcgcggttt tcggggcacg atctggcacg gcctggggct agaccgctac
     1621 ccgcagctac gggaggacta cttccgccac taccgccggc tggtcttcat gacccagagt
     1681 gggcagccgg aactgttgga caaagcccag acaatcgccg cgatgctgga cttgcctcta
     1741 gaggtgcgcc acacgggtta cggccaactg gaggcgcggc tggtggcgtg gatggcgagc
     1801 gcgagcttgc ttgaggacga cgcgccagcg gcgacatgag gtcaggactc agttaaccgg
     1861 cgtctcgacc acggcagtcg tctcccgacg cgccacagcc accgtggccg tgttcagcag
     1921 caacatcagc gccgcgccaa ccaccagaaa cggccccagc acttccagcc cgacacgctg
     1981 ggcgatgatg ccggccaaac cgggcagcag ggccaggccg atgctggccg cgccggtctg
     2041 cacggagatg gtgttggccg tgtgtcgccg gccgacgcga gcgggcgtgt gcgccgttag
     2101 cgttgggaag atgatcgaca gcgacagacc catcaggatg atggcggcca gaccggttgg
     2161 caacgtgcgc ggcagcaaca acagggccgc cagcaccacg ccaaccatgc acgtccgcag
     2221 cagccgctca atgccaatgc gcgcagcccc cgcaccgaac acaatccggc ccagggttga
     2281 catggcccag aagacgctgg tgattgttcc ggcgacgtag gcggacgcgc cgcgggcttc
     2341 agtgaaccag gtataagtcc actgaccagc tgttgtttcg acgcccgtat agagcatgaa
     2401 cagtaagatg ctcagccaga cgacgagcag gcgcaatgtt gctcgcatgg gcaccggccg
     2461 ggtatcctca gtcgcctggt tggcaccgcg aaagttcatc gtgccagcga cggtgataaa
     2521 gagcagggcc agaccgaggt ggacaccggc gacggccagg tagcccaacc gccagctgag
     2581 gccggtagca acgatggcag tcatcagtaa cggcccgatg gtggctccca cgccgaagct
     2641 ggcgtgcatc cagttcatcg tccggacgga atggtttgcg gcgacgtaga tattcaggga
     2701 tgtattgata actccctgcg ccaggcccca cagcagaccg gcgacaatca tggcgatcca
     2761 ggtcggggcg aggacatagc caatcgcagc caggacgccg accgcgttgg ccaacaacag
     2821 tgaccagcca acgcccagcc gcgccatgat ctgcgctgac aggaagctgc cggcgacaac
     2881 accggtcgtc gccgcggcca ggagggccgc cagggcatca agtggtaagc caaatgtgac
     2941 gcggatagat ggccaggcga tgctcagcaa gccacccatc atccccaagg caacgtagca
     3001 ggtaaaggcc agcaccagcg ggtagcggcc cctggctcgc atttcaatgg acatgtacat
     3061 gcatccagtc tggaattgcg ggtcgagttg cccgcgggtc aactggttaa gtatactgtt
     3121 ttccagcaaa gggggcattg tgaaacaaac gtcattggga gatacgggac tgtccgtcac
     3181 gccgatgggc ctgggcctgg ccgcgctggg ccggccgggc tacatcaatc tgggtcacgc
     3241 cacggatctg aagcgcgagt acgacgtcgc ggcgatggcc gagcacgcct atgccgtcct
     3301 cgacgtcgcc tggcaggcgg gcatccgcta cttcgacgtg gcccgctcct atggtcgggc
     3361 agaggagttc ctggccggct ggttgcgcgc gcgccatatc gcgccgggcc aggtatctgc
     3421 cggctccaag tggggctaca cctacacggc cgactggcag gtcttggccg agcgccacga
     3481 agtcaaagac cacagcctgc ccgtcctgcg ccgccaaatc ggcgaaagcc gcgccctcct
     3541 gggcgactac ctgagccttt accagattca ttcggccacg ctcgacagcg gtgtgctgga
     3601 aaacggcgag gtgctggccg agttggcccg gctgcgcgac ggcgggctgg tgattggcct
     3661 gtcgctcagc ggccctaacc aggcggaaac gctacgccgg gcgctggcca ttgagatagg
     3721 cggcgcgccg ctgttccgct ctgttcaggc aacgtggaac gtgctggccc aggaagctgg
     3781 cccggccctg gccgaggccc acaaccgcgg catgggcgtc atcgtgaagg aggcgctggc
     3841 caatggccgc ctgaccgagc gcaacatcga ccccgccttc gccgagcagc ggcggctgtt
     3901 gagcgcggca gcggccgaaa ccggggtcgg gctggatgcg ctggcgctgg cggcggtgct
     3961 ggttcagccc tgggccgacg tggttctcag cggcgcggcg cgggcggaac atctggcgtc
     4021 gaatgtcgcc gcggtcgcgc tggccacgcg ccgccgcgcc tggctacacg actggctgcg
     4081 gcacgcgcgc acgctggccg agccggccga cgtctactgg cgcacgcggg ccgcgttggc
     4141 ctggaactag ttctgcccgg gcagaggccc agacttgtcc ggctggcgcg gggccgcggt
     4201 tgtgccactg gtgccggcgt ctggcgcggc cggcgcagcg ccaggacgct ggtaggtggg
     4261 cacctgggtg ctgggcagtg gcccctcgcg cacggccgta gccgtgggca cgacgtcata
     4321 gcgctggccg ctgtcggaac gctccacctt agtgacagcg cgggccttgg ccagaatttg
     4381 cggcgcgacc tccgcgtcgg tcagatcaac ctcggtcaag tcggccccgg tcaggttggc
     4441 gttgctgagg ttggcccaac gcaggttggc cttgctcagg tcagcccggc tcagatcagc
     4501 gtcgcccaga atggctccgc gcaggattgc gcctcggagc atcgccccct tcagctgcgc
     4561 cgcgcgcaag tctgcgccgc tgaggcccgc ttcttcgagg ttggtacggt tcaggatggc
     4621 ccggttcaga ttggcgatgc tcaggttggc ccggctcaag tcgctgccag tcaggtcgga
     4681 gccgctcagg gacacgcggc ccatgatggc atcgcgcaga ttggcgttgg tcacgatggc
     4741 ctggttcatg accgcgccgc gcagattggc caggttgagg ttgctgtcgc gcaactcggc
     4801 gtcggtcagg tccgcctcgc tcagaatcgc cccgccgaac atggtgccgt tgagccgggc
     4861 gtggacgagt gttgctccgg ccaggttcga accgctgaag tccgccgccg ttgcctgcac
     4921 cccgcttagc gtggccttgt tgaggttggc cagactgagg ttggcgccgt ccagcgtcgc
     4981 gtcggtcagg ttagccccat tcaggcgcgc tcccttgagg ttcgcgccgg ccagattggc
     5041 ccgcgtcagg tcggcgccgg ccagattggc ctcgctcagg tcggcgtcac gcagatcggc
     5101 gtcggtcagc aacgccttgg tcatgatcgc gccgcgcaat tgggcgccgg caagcacagc
     5161 ctcgcgcagt gtcgcgccgc gcaggtagga ctcgctcaga tcggcccgcg tgagattggc
     5221 ccggtccagg tctgccccgc gtaggtcaga ggcgggcaag gcggtctcga ccaggcgcgc
     5281 ttcgtgcaag tccacattcc gcagactgat gccggccatg gcgacgccgg agaggtcgga
     5341 gccactcaga tcggcccgct gtagatcgac agccggctgc tcggcgctca gtagggagga
     5401 ttcatacaga aagcgcacga tgtggcctcg tccctcgctg ttcagttggc gcagcgtcat
     5461 caacgtatgg gcgcgggcgg cccgggcaac ctcgtcgtcg gccgcggctc cgcgcaggtt
     5521 gcgacccagt agcagttctg tcagccggtc gtagtaggaa tcgaccaggg cgcgctcgtc
     5581 gcgcagccgg gcgatctcca actgcgtgcg cttccacgtg cccgccagcc aaacggcggc
     5641 cgtgacgagc aatagcggga tcagcaacag gtttagccaa ttgagcagag ccagcgatcc
     5701 tcgttcgcgc accagaaact ggacgcccaa aaccgccagc gccagaatga cgatcaatat
     5761 caggacgacc ataatcgagc gcgcctgcga ggagcgtatt tcgcgatagt agcgggcaag
     5821 agattgacgt agcgattctg ccataaccgg ttagcctagt tttgggtcgc ccacaggttg
     5881 agcaactcct tcatgccatc acggatcact tgcgcgtcag cggccgcggg agagcgctgg
     5941 agatagacgt ccagataatg cgccgcctgt gtcatctgac gttgttggta gtagaccagg
     6001 cccaggtcgc gcacatgctc ggccacttcg ggctgcgttt ggcgcagcag gcggatgacg
     6061 cgcgccgctt ccttccaggc cgcagcccgc acgtagctgg agcgcagatt gttgagcatc
     6121 cgtgccagga tttcgcgggg cggcaggggc gcaaaccagg cgtcctccag cgcgcccccg
     6181 tagccgaccg ccaggcgaac caggtcgatg caatcggtca gatcgaggcg acggccgccg
     6241 tggaagggat cgagccagat gtcggcgtcg cggctatgga tgccgacgat gaaatgccct
     6301 ggcaggccga tgccgtaggc gggtatgccc agacggtggg cgatgtcgat aaagatgact
     6361 gatagcgaga tgggaatccc cagccgccga tccagcacct cattcaggta gctgttgcgg
     6421 gggtcgccat agtcctcggt attgccacga aacctgagag tgacaaagag atactcagcc
     6481 agttggtcgg cctgtctggc cacagggccg gccaaatcca cgtgctcggt ggccaactcc
     6541 gacagcgtat gcagcgcggt catgtagttg gcgacatcca accgtgggta ggcaagggtg
     6601 cgggcgaact ggagcgccgc ccgcggcacg ttcagttcgg tcaggcgcaa ctcgtcttgg
     6661 taaacccaat cactcatggc gaccattatg ggccattcgg agcagttttt ccatggggag
     6721 ttcggccgcc tccgccgtat cccgttcgtg ctacaaagcc tggccatgta catcgaagta
     6781 catctgggcg accgggttcg cctccgcaag aagcaccctt gcggcaatga tgaatgggac
     6841 atcatccgcc tgggggctga catcggcctc gtctgctgcc gctgtcgccg gcgttgaaga
     6901 caatgagcca ctgcccgtcg ctcgtcgcca gcac
//
LOCUS       DAOSMQ010000119         6858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11132_length_6858_cov_3.942231, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000119 DAOSMQ010000000
VERSION     DAOSMQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11132_length_6858_cov_3.942231"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1086
                     /locus_tag="PK829_05475"
     CDS             103..1086
                     /locus_tag="PK829_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOU40697.1"
                     /translation="MGYYLGLDVGATKTHALIADEAGRCRGFGAAGPGNYQSVGYDGL
                     ARALGEAFAGAAGMAGVAAADITAAGFGIGGYDWPSDRPAHLEAIATLGLGCPLDVVN
                     DALNGLLAGTSAGWGVNVTAGSSNNCRGLDRAGREGRIVGNGAFFGEFGGGLEIALRG
                     LHAVNYAWIRRGPPTALTAAYLAATGAPDVLALMEGLSFGGYALQPHLALAVFAAAAA
                     GDGVAAGILRWAGEELGWLAVAVLRQLDLQGEAVEVVQSGSVFDGGAAITAPMEAVVR
                     LHAPRARFIRLRTLPVIGAVTLAVATGGRLPYPLRDRLIATLPDVYPPAAG"
     gene            1359..3263
                     /gene="ftsH"
                     /locus_tag="PK829_05480"
     CDS             1359..3263
                     /gene="ftsH"
                     /locus_tag="PK829_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU40698.1"
                     /translation="MDDQPNENRPNIPTWVWILTLFAIILGVQLWLSGKFSGPEQISL
                     QEVADHIRDGEVEQIAVTGDRLQVTLDDGRIFGAVKSPSDSLIETFQYYGISAEDLSN
                     KLIVHDQSTWNTLLSLAFTIGPVLLLLWIFSRGFRQMQGGGGNNIFGFGRSRARNLTD
                     ANRPTVTFEDVAGVEEAKLELAEVVQFLREPEKFVQVGARIPKGVLMVGPPGTGKTLL
                     ARAVAGEAGVPFFHISGSEFVEMFVGVGASRVRDLFEKAKQAAPSIVFVDEIDAVGRQ
                     RGAGLGGGHDEREQTLNQILVEMDGFDNETNVIVIAATNRADILDPALLRPGRFDRKV
                     FVDLPDVAGREKILAVHARGKPIASDVSFKEISRLTAGFSGADLENLINEAAIFAARR
                     SKRTISLPEFQDAFDRVVMGPERQSRVMSEDDKLTVAYHEAGHAVVSFYLTNTDPVQK
                     ITIVPRGRAGGFVMSLPEDRAVHSREFFEDQIAMALGGRAAEEHFFGRITTGAAADLQ
                     QATRLARAMVMEYGMSDRLGLPTYGGGSGSPFVGREMGWFGSGRDYSEEAAREIDAEV
                     KRLLDENYNRAGTIIQQNHDRMVYLATTLIDVETLDRPAFEKLMNEMTPSNGQVNAPP
                     LSSPQPAQAL"
     gene            3390..4178
                     /locus_tag="PK829_05485"
     CDS             3390..4178
                     /locus_tag="PK829_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOU40699.1"
                     /translation="MTQPDEPVTLQQAGPVLTVTLNRPELHNALNPALIAGLTQAFAE
                     RAQAAGVRVVVLTGRGRSFCAGADLTAMRAAADFTFEENIADGEAIFDLMLAIDRCPR
                     PVVGRVNGPAIGGGVGLVSCCDIVVAVERAVFAFSETRLGIVPAVISPFVLARIGPSA
                     ARELFLTGERFDARRAQAIGLVGHVVAETDLDEAVAERVGQLLQAAPGAQATAKDLIH
                     AVAFQPQASLREATAATIAARRASDEGREGMSAFLEKRQPWWQE"
     gene            4207..5100
                     /locus_tag="PK829_05490"
     CDS             4207..5100
                     /locus_tag="PK829_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU40700.1"
                     /translation="MKLAIFSDIHGNWPALAATMADIDAWRPDLVLVAGDVVNSGPSN
                     VACWDAIRRRAAADGWVVLRGNHEEYVAEWLGYTGPRAGPAYDRIRLSEWTFRQFNDA
                     AAVAAMAALPERWDFAAPDGSTLAALHASRLGNRAGIYPFTTDDEARARMIPGAAVFI
                     TAHTHIPHRRTLDGTQIVNVGSVGLPGDGDGRAAYARLTWRRARGWSAEVVRVAYDRA
                     AAERDFFASGFIDTAGPEAEMALVEFRAARDARTRWRAIYGEQVLRGELGLAASIRRF
                     LEETEFRPFMLPEKTAQTAAC"
     gene            5262..6083
                     /locus_tag="PK829_05495"
     CDS             5262..6083
                     /locus_tag="PK829_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40701.1"
                     /translation="MSSLNLLLSGTILPWLLGLLLVFAALALLLSLKLWRATKRSPYF
                     FMRQQAAKRMQSYLSICAVLVLMAVGVGYYGWRTPIDPTLRMALISDAKPVSADIQEL
                     FSQPSTAELTLESDMASAQSGGVANFLLSTDESAALSPELPAAYDRFEPQAELTEATK
                     LGEVVLSNDVTDEYEAINPANVFGEGFYTLYATFAYEGMANGMEWAWVWRHNGKVIDG
                     GNELWAYGDDGPGYIYLSPEEGFQEGRYSLEIWVNGELLGQAAATMNDAAISAGN"
     gene            complement(6139..>6858)
                     /locus_tag="PK829_05500"
     CDS             complement(6139..>6858)
                     /locus_tag="PK829_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOU40702.1"
                     /translation="QALGIAAFPTLAALAVRAAHREMRGILTDSLRLVLFLGLPATAL
                     LALLAEPYVAILFQRGLFDAQATALVATALRFYALGLIALTALEVVARGFYALSDTLT
                     PVLSGGLQIAVMWALSLWFRDSLFPALGWRPLGGLALSFSLSNILEVGLLLWLLRRRL
                     GGLNGGALLEGTARMGAATLAMGVAIVATVAPLPAGAIWARALVGTAAGGLVYLLACW
                     LLRVTELRQLVGLARRRLGRG"
BASE COUNT         1133 a         2424 c         2186 g         1115 t
ORIGIN      
        1 atcagccgcg agatgcgcta cttcctgcgg gcgtcggaca tccatctgat cggctaccgt
       61 ccgttgcgcg aggtcatggc cgcggccgcg ttctgagcag tcatgggcta ctacctgggc
      121 ctcgacgtag gcgccaccaa gacccacgcc ctcatcgccg acgaggccgg gcgctgccgt
      181 ggtttcgggg cggccgggcc gggcaactac cagagcgtcg gctacgacgg actggcgcgg
      241 gcgctgggcg aggccttcgc cggcgcggcg ggcatggccg gcgtggccgc ggccgacatc
      301 accgccgccg gcttcggcat cggcggttac gactggccct ccgaccgccc cgcccacctg
      361 gaagccatcg ccacgctggg cctgggctgc ccgctcgatg tcgtcaacga cgccctcaac
      421 ggcctgctgg ccggaaccag cgccggctgg ggcgtcaacg tcaccgccgg ctcgtccaac
      481 aattgccgcg gccttgaccg cgccggccgc gaagggcgca tcgttggcaa cggcgcgttc
      541 ttcggcgagt ttggtggcgg cctggagatc gccctgcgcg gtctgcacgc cgtcaactac
      601 gcctggattc gccgcggccc accgacggcc ctgacggccg cctacctggc cgccaccggc
      661 gcgcccgacg tgctcgccct gatggagggg ttgtccttcg gcggctatgc cctccagccc
      721 cacctggcgc tggcggtctt tgccgccgcg gccgccggcg acggcgttgc cgccggcatc
      781 ctgcgctggg cgggcgagga actgggctgg ctggccgtgg ccgtgctccg ccaactcgat
      841 ttgcagggcg aggccgtgga ggtcgtccaa tcgggcagcg tcttcgacgg cggcgcggcc
      901 atcaccgcgc cgatggaggc cgtcgtccgc ctccacgctc cacgcgcccg gttcatccgc
      961 ctgcggacgc tgccggtgat cggcgcggtc acactggccg tggccaccgg cggccggttg
     1021 ccctatcctc tgcgcgacag gctcatcgcc accttgccgg atgtctatcc accggccgcc
     1081 ggctgacgcc cggccactac ggcgacagtg gtcgtggccc actgtggccc accgcctcgc
     1141 ccgcgggccg ggcgagccgc aaccagccgc ttctatagat aatcaattgg taaacactta
     1201 tgactatcag atagaagttt ggtactctca tagggtaggg gttgagcctg gctcggccct
     1261 gttgatggat tcaacacgaa tgaccggcct gctgatccca tgcgggcggc accttaacca
     1321 ggttagcagt cggttaccct tgcgggagta gtgcgactat ggatgatcaa ccaaacgaga
     1381 atcgccccaa tatccccacg tgggtttgga ttctgacgct cttcgccatc attctggggg
     1441 tgcagctttg gctcagcggc aagttcagcg gcccagagca gatcagccta caggaagtgg
     1501 ccgaccacat tcgcgacggt gaagtcgagc aaatcgccgt cactggcgac cggttgcaag
     1561 tgactctaga tgacggccgc atctttgggg ccgtcaagtc tccgtccgac agtcttatcg
     1621 agacgttcca gtactacggc atctccgccg aagacctcag caacaagctg attgtccacg
     1681 accaatcgac ctggaatacc ctgctcagcc tggctttcac catcggcccg gtgctgctgc
     1741 tgctatggat tttcagtcgt ggcttccggc agatgcaggg tggcggtggc aacaacatct
     1801 tcggcttcgg ccgcagccgc gcgcgcaacc tgaccgacgc caatcggccg accgtcacct
     1861 tcgaggacgt ggccggcgtg gaggaggcca aactggaact ggctgaggtc gtccagttcc
     1921 tgcgcgagcc ggagaagttc gtccaggtcg gcgcgcgcat ccccaagggc gtcctcatgg
     1981 tcggcccgcc cggcacgggc aaaacgctgt tggcccgcgc cgtggccggc gaggcgggcg
     2041 tgcctttctt ccacatctcc ggctcggagt ttgtggagat gtttgtcggc gtcggcgcca
     2101 gccgcgtgcg cgacctattc gagaaagcca agcaggccgc cccgtcgatc gtctttgtgg
     2161 acgagatcga cgccgttggc cgccagcgcg gcgcgggcct gggcggcggc cacgacgaac
     2221 gcgagcaaac actgaaccag attctggtgg agatggacgg ctttgacaac gagaccaacg
     2281 tcatcgtcat cgccgccacc aaccgggccg acatccttga cccggccctg ctgcgccccg
     2341 gccgtttcga ccgtaaagtt ttcgtcgatc tacccgacgt tgccggccgc gagaagattc
     2401 tggctgtcca cgcccgtggc aagcccattg ccagcgacgt cagcttcaag gaaatctcgc
     2461 gcctgacggc tggcttctcc ggggccgacc tggagaacct cattaacgag gcggccatct
     2521 tcgccgcccg gcgcagcaaa cgcaccatta gcctgccgga gtttcaggac gccttcgatc
     2581 gcgtcgtcat ggggccggaa cggcaaagcc gcgtcatgag cgaggacgac aagctgaccg
     2641 tggcctacca cgaagccggc cacgccgtgg tcagcttcta tttgaccaac accgacccgg
     2701 tgcagaagat caccatcgtg ccgcgcgggc gggctggcgg ctttgtcatg tcgctgccgg
     2761 aggaccgcgc cgtccatagc cgcgagttct ttgaagacca gatcgccatg gccctcggcg
     2821 ggcgggcggc cgaggagcat ttctttggcc gcatcaccac cggcgcggcg gccgacttgc
     2881 agcaggccac gcgcctggcg cgggccatgg tcatggaata cggcatgtcc gaccggctcg
     2941 gcctgccaac gtatggcggc ggctccggca gtccttttgt cggccgcgag atgggctggt
     3001 tcggcagcgg ccgggactat agcgaggaag ccgctcgcga gatcgacgcc gaggtgaagc
     3061 ggctgctgga cgagaactac aaccgcgccg gaaccatcat ccagcagaac cacgaccgca
     3121 tggtttacct ggcgacgaca ctcatcgacg tggagacgct cgaccggccg gcgttcgaga
     3181 agctgatgaa cgaaatgacg cccagcaacg gccaggtcaa cgccccacca ctgtctagcc
     3241 cgcagccggc gcaagcccta taggccagca cctgccacca gcagctatca ggctcttcgg
     3301 ggcgggtcgt caacccgccc ctttcgtttt cgccccgcgc tgcatcgccg ttgccacgcc
     3361 ccgcgcccag tctataatcg ggccacacca tgacccaacc agacgaaccc gttaccctgc
     3421 aacaggccgg ccccgtgctg accgtcacgc tcaaccggcc agaactccat aacgccctga
     3481 acccggccct catcgccggg ctgacacagg ccttcgccga gcgcgcccag gcggccggcg
     3541 tgcgcgtggt cgtcctgacc ggccgcgggc gctccttctg cgccggggcc gacctgaccg
     3601 ccatgcgcgc cgcggccgac ttcactttcg aggagaacat tgccgacggc gaagccatct
     3661 tcgacctcat gctggccatc gatcgctgcc cgcggccggt cgtcggccgt gtcaacggcc
     3721 cggccatcgg tggcggcgtg ggattggtga gttgctgcga cattgttgtg gccgtcgagc
     3781 gggccgtgtt cgcctttagc gagacgcggc tgggcatcgt cccggcggtc atctccccct
     3841 ttgtcctggc ccgcatcggt cccagcgccg cccgcgagtt gtttctgact ggcgagcgct
     3901 tcgacgcccg ccgcgcccag gccatcggcc ttgttggcca cgttgtcgcc gagaccgacc
     3961 tggatgaagc cgtggccgag cgtgtcgggc agctgctgca agccgcgccg ggcgcgcagg
     4021 ccacggccaa ggaccttatc cacgccgtcg ccttccagcc ccaggcgagc ctgcgcgagg
     4081 ccacggcggc gaccatcgcc gcccgccgtg ccagcgacga ggggcgcgag ggcatgagcg
     4141 ccttcctgga gaagcgccag ccgtggtggc aggagtaggg cggtttggca aaccgcccca
     4201 caatcgatga agctcgccat tttctccgac atccacggca actggcccgc gctggccgcg
     4261 acgatggccg acatcgacgc ctggcgacct gacctcgtgc tcgtcgccgg tgacgtcgtc
     4321 aacagcggcc ccagcaacgt cgcctgctgg gacgccattc gccgccgcgc cgcggccgac
     4381 ggctgggtcg tcctgcgcgg caaccacgag gaatatgtcg ccgagtggct cggctacact
     4441 ggcccgcgcg ccggcccggc ctacgaccgt atccgcctga gcgagtggac gtttcgccag
     4501 ttcaatgacg ccgccgccgt ggccgccatg gccgcgctgc cggaacgctg ggacttcgcc
     4561 gcgcccgatg gctcaaccct ggccgctctc cacgcctcgc ggctgggcaa ccgggcgggc
     4621 atctacccct ttacaaccga cgacgaggcc cgcgccagga tgatccccgg cgcggccgtc
     4681 ttcatcaccg cccacaccca catcccccac cggcgcacgc tcgacggcac gcagatcgtc
     4741 aacgtcggct cagtcggcct gccgggcgac ggcgacggcc gggctgccta cgcccgcctg
     4801 acatggcggc gcgcccgcgg ctggtcagcg gaggtcgtcc gcgtggccta cgaccgcgcc
     4861 gcggccgagc gcgacttctt tgcttccggc ttcatcgaca ccgccggccc cgaagcagag
     4921 atggccctgg tagaattccg cgccgcccgt gacgcgcgca cacgctggcg ggctatttac
     4981 ggcgaacaag tcctccgggg agagttggga ctggccgcgt caatccgacg gtttttagaa
     5041 gagactgagt ttaggccatt catgctgccg gagaagacgg cccaaacagc cgcttgttag
     5101 ctttttcact tatcttcttc tcatgaacgg tgggcctata tgtggcacaa gagcggcaga
     5161 atgggggcat tgccatccat tagaagcctc ggctggcctc aacgcttgct aatcggtgag
     5221 ccgctatcta taatgggcag tgaatctgga agaggaattg tttgagtagt cttaatttgc
     5281 tgctgtccgg taccatccta ccctggctgt tggggttgct gctggtgttc gctgccctgg
     5341 ctttgttgct ttcgctgaag ctgtggcggg ccaccaagcg gtcgccctat ttctttatgc
     5401 gccagcaagc cgccaagcgg atgcagtcct atctgtccat ctgcgcggtg ctggtgctca
     5461 tggccgttgg cgtgggctac tacggctggc gcacacccat tgacccgacc ctgcgcatgg
     5521 cgctgattag cgacgccaag ccggtctcgg ccgacatcca ggagctattt agccagccct
     5581 ccacagctga gttgactctg gagagcgaca tggcttccgc ccaaagcggc ggcgtggcca
     5641 acttcctgct cagcaccgac gaatcagccg ccctctcccc cgaattaccc gcggcctacg
     5701 accgctttga gccgcaagcc gaactgactg aagcgaccaa gctgggcgag gttgtcctct
     5761 ccaacgacgt caccgacgag tacgaagcca ttaacccggc caacgtcttt ggcgaaggct
     5821 tttacaccct ctacgccacg ttcgcctacg agggcatggc caacggcatg gagtgggcct
     5881 gggtctggcg gcacaacggc aaggtcatcg acggcggcaa cgaactgtgg gcctatggcg
     5941 atgacggccc cggctacatc tacctcagcc ctgaagaggg tttccaggaa ggccgctact
     6001 cgctggagat ttgggtcaac ggcgagcttc tcggccaggc tgcggcgact atgaacgacg
     6061 cggctatttc ggcgggcaac tagcgccatc gagcgccgcc ccgcacaccc aaccggctca
     6121 gcgccggttt tttgttgcct agccccgccc cagccggcgg cgggccagcc ccaccaattg
     6181 ccgcaactcc gtcacccgca gtagccagca ggccagcaga tagaccagcc cacccgcggc
     6241 cgtgcccacc agcgcccgcg cccagatagc cccggccggc agcggcgcga cggtggcgac
     6301 aatggccacg cccatggcca gcgtagccgc gcccatccgc gctgtgccct ccagcagcgc
     6361 gccgccgttc agcccgccca gacgccgccg caacagccac agcagcagcc ccacttccag
     6421 gatattggac aggctgaaac tcagagccag cccgcccagc ggccgccagc ccagcgccgg
     6481 gaacaggctg tcgcggaacc acaagctcag tgcccacatg acggcgatct gtaggccgcc
     6541 cgacagcacc ggcgtcagcg tatcgctgag ggcatagaag ccacgggcga cgacctccag
     6601 cgcggtcagg gcgatcaagc ccagggcata gaagcgcaac gccgtggcca ccagcgccgt
     6661 cgcctgggca tcgaacaggc cgcgctggaa gaggatggcc acgtagggct cagccagcag
     6721 cgccagcagc gccgtcgccg gcagtcccaa gaagagcacc agccgcagcg agtcggtcag
     6781 gatgccgcgc atctcccggt gggcggcgcg gacggccagc gccgccagcg tcgggaaggc
     6841 ggcgatgccc agcgcctg
//
LOCUS       DAOSMQ010000120         6784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11287_length_6784_cov_4.103879, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000120 DAOSMQ010000000
VERSION     DAOSMQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11287_length_6784_cov_4.103879"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PK829_05505"
     CDS             complement(<1..1531)
                     /locus_tag="PK829_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOU40703.1"
                     /translation="MTSKRRTWGQFATPVDVADVLLGFCLRRPDDRVLDPSCGDGALL
                     RRATRWRGWLSEEAAAPPGEALYGVELDEDAAARAATAAGATVVPANFLALDPTAPLA
                     ALGGRPLGNFDALIGNPPYTRAEWIGRLSAAAQLPLFGDDPTARHVRPYAVPGELAAA
                     LSGRAGLHAYFLLHSLSFLRPRGRLGFVMPNGWLDIAYGAALKRFLLDHFRVLAIIES
                     AVERWFAGASVNTCILILERADEAGARDDNRVRLARLRRPLHELLGVAAGDSRRVAAV
                     EQLVGRLLPGGDRLAGDIAVRVGRQGDLAAEERWGALLRAPAVYLRPPSPALVPLGRW
                     ATIRRGHTTGANDFFYLDARRATALGLAPRFRRPLLKSLRGLTRPRLDTADCRHELLL
                     IPADAPLEGDLADYVASGEARGIHRRTTCAGRQPWYSLLEQPGSHLLLAKGVWQRHFA
                     PLADEPVAIDQQIYGLRLPAEWLAAAAALLNSVWFALQCELRGRVNLGEGVLWLAAYE
                     LA"
     gene            1586..2059
                     /locus_tag="PK829_05510"
     CDS             1586..2059
                     /locus_tag="PK829_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40704.1"
                     /translation="MNTLSTSAFLKSLPEALRPLLPPPLQGFQWRLQWSSLLQIHYGE
                     ASLHYEIVHVGERLGRSRTGWELGFHCESRDHALNRFLLDGFRRHLFEIKAELGDSIE
                     AEMWDRGWTKIYEVYADESLTEAYRDAVAARLAAIMACLHPVFVDLRGAAAALYR"
     gene            complement(2158..5862)
                     /locus_tag="PK829_05515"
     CDS             complement(2158..5862)
                     /locus_tag="PK829_05515"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40705.1"
                     /translation="MNTKATLRIFLMMTALLLGMFTLVATRPAAAAPDAFGLTTKVEP
                     AGKGTVDVSPPPPYSENQVVSLTATPIAGWTFDKWVLASDLVWWDENWDYRVTVTAAA
                     AGYARKDKPAEVALNFTQLWSSLGKTGTLDPNSIRVVEVDGSDAVIDDTVPFQFDKAK
                     DFNAASKAAGTLVLIMEGNTPAGATRRYHVYFDVTGKGFAAPNVPSQITFNPSATDQG
                     KTAYQLDTPVGTYFYHTKGGGFSSFNDLGGNDWINWNSAPENAGDFRGIPNLVHPNNG
                     GYFHPGRTGMTTTLISQGPIKVTFESFEANNPPAGRDRWRGIWEVYPTYVNFTIVSAP
                     YNYYFLYEGTPGGALQINSDFVVRSTGVQTLANVAWEEDLANEEWAFVADPTVGRALY
                     MASHTDDLKTDSFGQQDGVMTKLGFGRKGIAPLLDKTIVPRQFTWGIMNEVTFDAGKP
                     VIYNAYKDLNVSVGAAEARSGASLGSTNPVSFTITGQHTITAVFKPQQFTVNVTTSGQ
                     GSVTKTPNKATYALNEQVTLTATPAVDWAFAGWSGDLTGSDNPATVTVTKNMNITATF
                     AQSFTITTSVNPAEGGSITLNPSKPTYQPGEQVQVTATANSGYTFTGWGGALSGSNPT
                     ETVTVNSNLNIIANFSSATYTFSATANGNGSVSWEPVKPLYGAGDVVTVTATPATDYY
                     FIGWTGDVNSSTNPLQVTISGNTSIVANFMPVVCYTLTTIVNPPDSGTVTIEPPSNCE
                     GGGYTVGTMVTLTAIPGTQKRFVGWSGGITGSISPIQFPITGDMTVIATFEDDVYPLT
                     TTVVGSGAIAKSPDQPAGYFIGQEVMLTALPASGSEFVGWSGDVTGTSPTVPITITGA
                     MSVTATFTTAGPFTLTVNTAGDGSGTVQISPEKAEYTYGELVELTPVPAPDSVFAGWS
                     GDASGAARPLRVTMTGDKTITATFIVPAGPFSDNFNSCTLANQWSAEQMGDATMSYTG
                     RSLKIQIPEGSDHYINRSLNNAPRLMQDADNRNFEIVAKFDSDLTQDYQTQGILIEGT
                     TATGVATMLRIDFTYNSGVVSLYSGAWTPGKLSQKFSLPIAAGDANYLRVNRTGDKWT
                     LSYSATGADNNWQAAGDFKYIMTVSRAGVFAGNLKPSGGVAPALTTEIDFFQNTAEGP
                     MVEDTPLLTINKVGNGTVTASPSIDQLTCGQTVRLTATPLPGGTFDGWSGDATGTQNP
                     LSMLLNRPRTVTATFTAPDTFTLSLPVIIR"
     gene            6397..>6784
                     /locus_tag="PK829_05520"
     CDS             6397..>6784
                     /locus_tag="PK829_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40706.1"
                     /translation="MGATILLASWPWLHGQVWAEATTPAPAAHDELSWQHLSTANGDL
                     EAPSGSLQQVLTLILDVDHDGLNDIVIGARRGSGPSLVWYRRQASGWERRVIEPQALK
                     MEAGGAAYDIDDAGDLDLVDGGNNQTN"
BASE COUNT         1085 a         2091 c         2366 g         1242 t
ORIGIN      
        1 cggccagttc atacgccgcc agccagagga cgccctcgcc caggttgact cgcccgcgca
       61 attcgcattg caaggcgaac cagacgctat tgagcagtgc cgccgccgcc gccagccact
      121 cggccggcag gcgcaggccg tagatctgct ggtcgatggc caccggctcg tcggccaggg
      181 gggcaaagtg gcgctgccag acgcccttgg ccagcagcag atggctgccg ggctgctcca
      241 gcaggctgta ccagggctgc cgcccggcgc aggtcgtccg ccggtggatg ccgcgcgctt
      301 cgccggaggc aacgtagtcg gccaggtcgc cctccagcgg cgcgtcggcc gggatgagca
      361 gcagttcgtg gcggcagtcg gccgtgtcca gccgcgggcg cgtaaggccg cgcagcgact
      421 tcagcagcgg ccggcggaag cgcggagcga ggccgagggc cgtcgcccgc cgcgcgtcca
      481 ggtagaagaa gtcgttggcc ccggtggtgt ggccgcgccg gatggtggcc cagcggccca
      541 gagggacgag cgcgggcgag ggcgggcgca gatagaccgc cggcgcgcgc aacagagcgc
      601 cccaccgctc ctccgcggcc agatcgccct gccggccgac gcgtacggcg atgtcgcccg
      661 ccagccgatc gccgccgggc aacaggcggc cgacgagttg ctccacggcg gccacgcgcc
      721 gcgagtcgcc ggcggcgacg ccgagtaact cgtgcaacgg ccggcgcagc cgcgccagcc
      781 ggacgcgatt gtcgtctcgc gccccggcct cgtcggctcg ctccagaatg agaatgcagg
      841 tgttgacgct tgcgccggca aaccagcgct ccacggccga ttcgatgatc gccagcacgc
      901 ggaagtggtc gagcaggaag cgcttcaggg ccgcgccata ggcgatatcg agccagccgt
      961 tgggcatgac gaagcccagc cggccgcgcg ggcgcaggaa ggacaggctg tggagcagga
     1021 agtaggcatg caggccagcc cggccggaga gcgccgccgc cagttcgccg ggcacggcat
     1081 agggccggac gtggcgcgcg gttgggtcgt cgccgaagag gggcaactgc gcggcggcgc
     1141 tgaggcggcc gatccactcg gcgcgggtgt agggcggatt gccgatgagc gcgtcgaagt
     1201 tgcccaacgg ccggccgccg agcgccgcca gtggcgcggt tgggtccaaa gcgaggaagt
     1261 tggccggcac gacggtggcc ccggccgccg tcgcggcgcg ggcggcggcg tcctcgtcca
     1321 gttccacgcc atagagcgcc tcgcccggcg gcgcggcggc ttcttccgag agccacccgc
     1381 gccagcgcgt tgcccggcgc aacagcgcgc cgtcgccgca actggggtcg agcacccggt
     1441 cgtctggccg gcgcagacag aagcccagca acacgtcggc cacatctacg ggtgtggcga
     1501 actggcccca ggtgcgtcgc ttgctggtca ttttttcctc cgcgcggttg gggaacggac
     1561 tggcaggcat tatactacag agacgatgaa cacgttatcg acaagtgcct ttctgaagtc
     1621 attgccggag gcgctgcggc cgctgctgcc gccacccctg cagggcttcc agtggcgtct
     1681 gcaatggtcc tcgctgttgc agatccacta tggcgaggcg tcgctccact acgagattgt
     1741 ccacgtgggc gagcggctgg gccggtcgcg caccggctgg gaactgggct tccattgcga
     1801 gtcgcgcgac cacgccctca accgcttcct gctcgacggc ttccgccgcc acctgttcga
     1861 gatcaaggcc gaactgggcg acagcattga ggcagaaatg tgggatcggg gctggacgaa
     1921 gatctatgag gtctacgccg acgagtcgct caccgaagcc taccgcgacg ccgtggccgc
     1981 gcggctggcg gcgatcatgg cctgcctgca ccccgtgttc gttgacctgc gcggcgcggc
     2041 ggcggcgctc taccgctaag cggccggccg cgccgggaaa gccaaccgcg gcgggcggtg
     2101 gggccaaacc ccatcgcccg ccgcggctat ttgcacggcg acgcagcgcg ccgggcgcta
     2161 ccggatgatc accggcagag aaagcgtaaa ggtgtcgggc gcggtgaagg tggccgtcac
     2221 cgtccgcggc cggttgagaa gcatcgacag cggattctgc gtgccggtgg cgtcgccgct
     2281 ccagccgtcg aacgtgccgc cgggcagggg cgtggccgtc agccgcaccg tctggccgca
     2341 ggtcagttgg tcgatggacg ggctggccgt gaccgtgcca ttgccgacct tgttgatcgt
     2401 cagcaggggc gtgtcttcga ccattggccc ttcggccgtg ttctggaaga agtcgatctc
     2461 agtcgtgagg gccggggcca cgccgccgga cggcttcagg ttcccggcaa agacgcccgc
     2521 acggctgacc gtcatgatgt acttaaagtc gccggccgcc tgccagttgt tatcggctcc
     2581 ggtggcgcta tagctgagcg tccacttgtc gccggtgcga ttgacacgca ggtagttggc
     2641 gtcgcccgcg gcgatgggca ggctgaactt ctgcgacagc ttgcccggcg tccacgcgcc
     2701 ggagtagagg ctgacgacgc cggaattata cgtgaagtca atccgtagca ttgtggccac
     2761 gccggtcgca gtcgtgccct cgatcaggat gccctgcgtc tggtagtctt gggtgaggtc
     2821 agagtcgaat ttggcgacga tctcgaagtt gcgattgtcg gcgtcctgca tgagacgcgg
     2881 ggcgttgttc aggctcctgt taatgtagtg gtcgctgcct tcgggaatct gaatcttcag
     2941 cgaccggccg gtgtagctca tggtggcgtc gcccatttgc tcggccgacc attggttggc
     3001 cagggtgcag ctgttgaagt tgtcgctgaa cggccccgcg ggaacgatga aggtggcagt
     3061 gatggtcttg tcgccggtca tcgtcacccg gagcggccgg gccgccccgc tggcgtcccc
     3121 cgaccacccg gcaaagacgg aatcgggcgc gggcacaggc gtcaattcga cgagctcgcc
     3181 gtaggtgtac tcggccttct ccgggctgat ctggacggtg ccgctgccgt cgccggccgt
     3241 gttgacggtc aacgtaaacg gcccggcggt ggtgaaggtg gcggtgacgc tcatcgcccc
     3301 ggtgatggtg atgggcacgg tcggacttgt gcccgtgaca tcgcccgacc acccgacaaa
     3361 ctccgagccg ctggcgggca aagccgtcag catcacttcc tggccgatga agtagccggc
     3421 tggctggtcg ggcgacttgg caatcgcgcc gctgccgacc acggtcgtcg tcagcgggta
     3481 gacgtcatcc tcgaaggtag caatgactgt catgtcgccg gtgatcggga actgaattgg
     3541 cgagatgcta ccggtgatgc cgccgctcca gccgacgaag cgcttctgag tgccagggat
     3601 ggccgtcagc gtcaccatgg tgccaacggt gtagccgccg ccttcacagt tggacggcgg
     3661 ctcaatcgtc accgtaccgc tgtccggtgg gttgactatg gtcgtcagcg tatagcagac
     3721 cacgggcatg aaattggcga cgatgctggt gttaccgctg atggtcactt gcagggggtt
     3781 ggtgctggaa ttaacgtcac ccgtccagcc aatgaagtag tagtcggtcg cgggcgtagc
     3841 cgtgacggta acgacatcgc cggccccata gaggggcttg accggctccc aggacacgct
     3901 accgttgcca tttgcagtag ccgagaaggt atacgtggcc gacgagaaat tggcgatgat
     3961 gttcaggttg ctgttgaccg tgacggtctc tgtcgggtta ctgccactca gagcgccgcc
     4021 ccagccggtg aaggtatagc cgctgttggc cgtggctgtc acctgcactt gttcgcccgg
     4081 ttggtacgtt ggcttgctgg ggttcagggt gatactgccg ccctcggccg ggttaacgct
     4141 ggtggtgatg gtgaacgact gggcgaacgt cgccgtgatg ttcatgttct tggtgacggt
     4201 gaccgtcgcc ggattgtccg aacctgtcag gtcgcccgac cagccggcaa aggcccagtc
     4261 cacagcgggc gtggccgtca gggtcacttg ctcattgagg gcgtaggtcg ccttgttggg
     4321 cgtcttggtg accgagcctt gaccggaggt cgtcacattg acggtgaact gctgcggttt
     4381 gaagacagcg gtgatggtgt gctggccggt gatggtgaag ctgaccggat tggtgctgcc
     4441 cagcgacgcg ccagagcgcg cctcggccgc gcccacgctg acgttcaggt ccttgtaggc
     4501 gttatagatg accggcttgc ccgcgtcgaa ggtcacctca ttcatgatgc cccaggtgaa
     4561 ctggcgcggc acgatggtct tgtcgagcaa tggcgcgata cccttgcggc cgaagccgag
     4621 cttggtcatc acgccatcct gttgaccaaa tgaatcggtc ttcaggtcgt cggtgtggct
     4681 ggccatgtag agcgcccgcc ccaccgtcgg gtcggccaca aaggcccact cttcgttggc
     4741 caggtcttct tcccaggcga cgttggccag ggtttggacg ccggtgctgc gcacaacgaa
     4801 gtcgctgttg atctgcaacg cgccgccggg cgtaccctca tagaggaagt agtaattgta
     4861 gggcgcggag acgatggtga agttgacgta ggtgggatag acctcccaga ttccgcgcca
     4921 gcggtcgcgg ccggccggcg ggttattggc ctcgaagctc tcgaaggtca ccttgatcgg
     4981 tccttgcgag atcagcgtgg tggtcatgcc ggtgcggccg gggtggaagt agccgccgtt
     5041 gttggggtgg acaaggttgg gaatgccgcg gaagtcgccg gcattctctg gcgcggagtt
     5101 ccagttaatc cagtcgttgc cgcccaggtc attgaaactg gagaacccgc cgccttttgt
     5161 gtggtagaaa taggtgccga cgggcgtatc caattgatag gcggtcttgc cctggtccgt
     5221 ggcgctgggg ttgaaggtga tctggctggg cacgttgggc gcggcgaagc ccttgcccgt
     5281 cacgtcgaag taaacgtgat agcggcgcgt cgcgccggcg ggtgtattgc cctccatgat
     5341 cagcacgagc gtgccggccg ccttgctggc ggcattgaag tctttggctt tatcgaactg
     5401 gaagggcaca gtgtcatcga tgacggcatc gctgccatcc acctcgacca cgcggatcga
     5461 gttggggtct agcgtgccgg tcttgcccag cgacgaccag agctgggtga agttgagggc
     5521 gacctcggcc ggcttgtcct tgcgggcgta gccggcggcc gcggccgtca ccgtgacgcg
     5581 gtagtcccag ttctcgtccc accagaccag gtcgctggcc agaacccact tgtcgaacgt
     5641 ccagccggcg atgggcgtgg ccgtcaggct gacaacctga ttctcgctat agggcggcgg
     5701 cgggctgaca tcgacggtgc ccttgccggc tggctcgacc tttgtcgtca ggccaaacgc
     5761 atcgggcgcg gccgcggccg ggcgcgtggc cacgagggta aacatgccca gcagtagcgc
     5821 ggtcatcatc aaaaaaatgc gcagtgttgc ctttgtgttc ataactttct ccccaacgtt
     5881 tgcgacagcc ggcacccgga agccggttgc tgctagaggt gctcgccacg tcgctgaatt
     5941 tcgtcgtctt gcctggtggg tcgctcctga ctccagacgt ggggccgcgg ttgcggccat
     6001 ttttgtctct cgtagcatag tccgtctccc ttacggcatt ccttaacggc attcctggcg
     6061 tgtcgccgcg gccctgtggc caggggataa ggccacgcac gtcatattac tttctgggcc
     6121 gtgccatcgg gtcgcagaat atgaatcatt tgacggttga ccgtgtaggg agccggcggc
     6181 tattgttctg aaagcttggt ttgtccgtca tcaggccagg ggcaggcggt tggcaaccgt
     6241 tggttggcaa ccgttggttg gcaaccgttt tggtaatcac tttgtcctcc gtatcaacta
     6301 gttgacctgt agcggttggg ccttgcagcc cggccggttg aaaggctggg tgcgtttatg
     6361 acggcaagac gaacgcaacg attcgtattc cttgtggtgg gggccaccat tttgctcgcc
     6421 agttggccgt ggctccatgg ccaggtgtgg gctgaggcca ccacaccggc cccggccgcg
     6481 cacgacgaac tgagttggca gcacctctcg accgccaacg gcgacctgga agcgccatcg
     6541 ggcagtctcc agcaagtcct gacgctgatc ctggacgtag accacgacgg gctaaacgac
     6601 atcgtgatcg gcgcgcggcg cggttctggc ccttctttgg tctggtatcg tcgccaggcc
     6661 agcggatggg aacgccgggt cattgaaccg caggccctta agatggaggc tggtggcgcc
     6721 gcctacgata tcgacgacgc cggcgacctt gacctggtag acgggggcaa taatcagacc
     6781 aacc
//
LOCUS       DAOSMQ010000121         6782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11294_length_6782_cov_4.506021, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000121 DAOSMQ010000000
VERSION     DAOSMQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11294_length_6782_cov_4.506021"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..611
                     /locus_tag="PK829_05525"
     CDS             402..611
                     /locus_tag="PK829_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40707.1"
                     /translation="MGKVMGFLAGAVCGTLVGAVTALLLTPASGTELIREAEERWELT
                     LAEARRARDEKRRELEAQYRAETRA"
     gene            complement(608..>2444)
                     /locus_tag="PK829_05530"
     CDS             complement(608..>2444)
                     /locus_tag="PK829_05530"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOU40708.1"
                     /translation="GASTPTRPLPPAAVAPTPTAARPTLVVATAVPGALIDAQPPPAR
                     AQEDYERLFTTILPPLDPLALAAELDGVSEPAVTATATPAVGDWATFQTSEGPRQAEL
                     IYADDLAAYWVESGLSLDQAALIASAGWLRQQATPLLSALFGEATRLGLDGDPRVHIL
                     HLLGSPDADELGYFRDENQYPRWLESDSNEREMIYLNMARLTAGTPLYEGTLAHEMQH
                     LIQWSLDANEDAWLNEGLSQVAETLVGLDTVDATYYREQPYIRLDRWSDAAPDVDAHY
                     AGSYLYVLYLWEQAGDAALSELARQPANGLAAVRAVLAGHRPQLSWEEFTGDWAAAVA
                     LNDRSVDPRYGFRHPVDLGQAFLANRVRRLPFVATTTLDQMAVDMIDLDFSGPATLSF
                     AGDATAALIDGPPPAGEAFWFAPPGNSSRAQLTAEVDLTTAVAPALDFAVWHDLEPTY
                     DFAYLSASTDGGQTWTVISPGQSLYGPYGPAWGGPSAGWRQETVPLDDFAGGTVRLRF
                     DVMTDFEGLGRGFAVSGLAVSGVAIQPLWQADGFVETGAVLPQRWELRLIRKGAAPEV
                     VRLMPDASGRGEWAVDLGPDGGMLVVMPVTPLVVAPADYWLRVDR"
     assembly_gap    2445..2544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2813..3964
                     /locus_tag="PK829_05535"
     CDS             2813..3964
                     /locus_tag="PK829_05535"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU40709.1"
                     /translation="MRYAHIVGWGHYLPERVVTNDELSRLVDTTDEWIVQRTGIHTRH
                     IAAAHETTATLAFEAAARALAVADLHPSQIELIIVATSTPGHMFPSTACLVQDYLGAS
                     RAGAFDLGAACSGFVYALQMASQSIATGAVRNALVIGAETMSRVVDWSDRGTCVLFGD
                     GAGAVVLKGSSIPGGVMATTLRSDGSGADLLSLPALYHNPVPIAGAEFAHNGHHKNIV
                     DMDGRQVYRFATNVIASSVQDVLKQSGLTLDDIALIIPHQANVRIIETAARKMKLPLE
                     KFYLNLDHTGNTSAASIPVALCDAVSEGRLEPDDNIIFVGFGGGLTWGASLVKWDVTP
                     PQVSFIDHEWKRARYVMARGRSRLRRLSRRVEAAVARSSNGHKGDDSAE"
     gene            complement(3979..4641)
                     /locus_tag="PK829_05540"
     CDS             complement(3979..4641)
                     /locus_tag="PK829_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40710.1"
                     /translation="MSDITPPPGVPFDDETWRQLPGFLDNLPESVCLHVWADEQGEPA
                     EREATRLAQALSERSPRLSYRLFPRRQNYPYYPVIGVMGGTTVESRDDGLRIIGLPLG
                     YQMTSLIAAIQAVAFRGQTLEPATRVRLSRLPADADIAIELLTAAEDEWGGVAAKTVF
                     GMAAASPRVRAFLIMTDLFPEAAQRYSVTALPHVVVNRRVHFSGPPDEAELLRHIGLA
                     LK"
     gene            complement(4638..5447)
                     /locus_tag="PK829_05545"
     CDS             complement(4638..5447)
                     /locus_tag="PK829_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU40711.1"
                     /translation="MKHKQPSESAPEPEESEEVRRLRRELARANARLERLQQRKSSML
                     AMAAHDLRTPVAVIQGYAQLLELSLAAESDPAIREYLTTIIAHAASLRNGVESLVALD
                     AMDRGEVKIEPVRCDLVELVGQALAQVEGLMSVKGVELTLATPPEPLWVMADEDAAGR
                     ALYSLLSHAEKYTRERGRLTIAVAADGNQGRTSVHDPDRRLPAEVIARAFERVELGPE
                     GRAATRGTDLGLVVARKVAEAHGGHVAASSTEAGTTLSLSLPRASEEPAEQ"
     gene            complement(5493..>6782)
                     /gene="mutS"
                     /locus_tag="PK829_05550"
     CDS             complement(5493..>6782)
                     /gene="mutS"
                     /locus_tag="PK829_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU40712.1"
                     /translation="ASLEPRERQRSGIASLKVGFNKVFGYYIEVTHANTHLVPSDYIR
                     KQTLTNAERYITPELKEYETLILNAEERILQIERRLFSELCGFVGEHSARLLGTAAAL
                     AELDVAAALAEVAALNDYIRPTLSDPSQTGEIAIHIENGRHPVVEGALAEGSGLGARR
                     GERFVPNNTHFDDSNLIQIITGPNMSGKSTYLRQVALIALMAQVGSFVPATSADIALV
                     DRIFTRIGAHDELHAGRSTFMVEMVETAEILNHATHRSLLVLDEIGRGTSTYDGLSLA
                     WAIVEFLHNHPRLKPRTLFATHYHELVGLAGLLPDVANFNVAVSEEGGEVTFLHHIVP
                     GGADRSYGIHVAQLAGLPRDVILRANDILRELERRAPTTAVEPSRLSSSQQIALFPDS
                     SPFLDELQALDVNALTPLQALNKLYEWKQRYASQGSE"
BASE COUNT         1131 a         2191 c         2256 g         1104 t
ORIGIN      
        1 ttgcccagca tgtcaaccaa tgtgttgtga atactggtgt tcttaatgcc ggcggaagtg
       61 agcagcagtt tcataatctc tccttgtcgc ggtagtggcc ccctctgttt gatactgtag
      121 ccgaacaatc ctaattgtac gacgtgattt acccatgaca agacttgaca taatgacgta
      181 tgcagggcca ttcaatagga actcacgcaa agccgccaag gggcagaagg cgcaaagaaa
      241 aaacgacaga tgataaagct tcttctctgc gtcctttgcc tacttggcga ctttgcgtga
      301 gcgcttcttc caacttagaa atgccactga tagcgcattg gccactcctt ccctccacgc
      361 gctataatgg cggcatcatt cactgacaag gagcaaacgt aatgggcaaa gtgatgggct
      421 ttctggcggg ggctgtctgc gggacgctgg tcggcgcggt gacggcgctg ttgctgacgc
      481 cggcatcggg cacggaactc attcgcgagg ccgaggagcg ttgggaactg acgctggcgg
      541 aagcgcgccg cgcccgcgat gagaagcggc gcgagttgga ggcccagtac cgcgccgaga
      601 cgcgcgccta gcggtcgaca cgcagccaat agtcggccgg cgcgactacc agtggcgtca
      661 cgggcatcac caccagcatg cccccatccg gccccaggtc aaccgcccac tccccccggc
      721 cagacgcatc cggcatcagc cggacaacct ctggcgcggc ccctttccga atcaggcgca
      781 actcccaccg ctgtggcaac accgcgcccg tctccacgaa gccatcggcc tgccagagag
      841 gctgaatcgc gacaccgctc acggccagcc cgctgacagc gaagccgcgc cccagcccct
      901 cgaaatcggt catcacgtcg aagcgcaacc gcaccgtgcc gccggcgaag tcgtccaggg
      961 gcacggtctc ttgccgccag ccggcgctcg gcccgcccca ggccgggcca taggggccat
     1021 agagcgattg ccccggcgag atcaccgtcc acgtctgccc gccgtcggtg gaagccgaca
     1081 gataggcgaa gtcgtaggtc ggctccaggt catgccagac agcgaaatcc agcgccggcg
     1141 cgacggcggt ggtcagatcg acctcggccg tcaattgtgc ccggctgctg ttgcccggcg
     1201 gcgcaaacca gaaagcctcg cccgccggcg gcggcccgtc gatgagcgcc gcggtcgcgt
     1261 cgcccgcaaa ggacagtgtc gccgggccgc tgaagtcaag gtcgatcatg tccacggcca
     1321 tctggtctag cgtcgtcgtg gccacgaagg gtagtcgccg gacgcgattg gccaggaagg
     1381 cctggccgag atcgacgggg tggcggaagc catagcgcgg atcgaccgac cggtcgttta
     1441 gggcgacggc cgcggcccag tcgccggtga actcctccca gcttagctgc ggccggtggc
     1501 cggccagcac ggcgcgcacg gcggccagcc cgttggccgg ctggcgggcc agctccgaca
     1561 aggccgcgtc gccggcctgc tcccagagat agaggacata cagatagctg ccggcatagt
     1621 gggcgtctac gtccggcgcg gcgtccgacc agcggtcgag gcggatgtag ggctgttcgc
     1681 ggtagtaagt ggcatcaacc gtgtctagac cgaccagcgt ctcggccacc tgcgacagcc
     1741 cttcgttaag ccaggcgtcc tcgttggcat ccaggctcca ctgaatgagg tgctgcatct
     1801 cgtgggccag cgtaccctca tagagaggcg tcccggctgt cagacgggcc atattcaggt
     1861 agatcatctc tcgctcgttg gaatcgctct ccagccagcg cgggtactgg ttctcgtcgc
     1921 ggaagtagcc caattcgtcg gcgtcgggcg agccgaggag gtggagaata tggacgcggg
     1981 gatcgccgtc aaggcccagc cgggtggcct caccgaagag ggcgctgagc agcggcgtgg
     2041 cctgctggcg cagccacccg gccgaggcga tcagggcggc ctggtcaagt gacagaccac
     2101 tttccaccca gtaggctgcc aggtcgtcgg cgtagataag ttcggcctgg cgcggccctt
     2161 cggacgtctg gaaggtggcc cagtcgccga cggctggcgt agccgtggcg gtgacagccg
     2221 gctcgctgac gccgtccaac tcggcggcca gagccaatgg gtcgaggggc ggcaagatcg
     2281 tcgtgaacag acgctcgtag tcctcttgcg cccgcgctgg cggcggttgc gcgtcgatca
     2341 acgcgcccgg cacggctgtg gccacgacca gcgtcggccg ggcggctgtc ggcgtcgggg
     2401 caacggccgc cggcggcagt ggacgggtag gcgtggacgc gccgnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnngcgtgg acgcgccggg cgcggctaca gcaggcgtga
     2581 tggtcatggg agcggcctgt ggcggccggg cgcgcaacag ccaccagccg gccaggacgg
     2641 ccgcgccgag cagcaggctc aggcaaccaa tgacaataaa cgtgcccctc gcgggcggcg
     2701 attgctggtt cactcgcgcc ggattgtaac cgccggtcga tcggcggcgc aactcatggc
     2761 ccagggtagg gtttcaactt tccgagacgg ttgctcaacg gaatcggaca tgatgcgcta
     2821 cgcccatatc gtcggctggg gtcactatct gcctgagcgg gttgtgacca acgacgaact
     2881 atcccgcctg gtggacacga cggacgaatg gatcgtccag cgcacgggca tccacacgcg
     2941 ccacatcgcc gcggcccacg agacaacggc cacgctggcc ttcgaggccg ctgcccgcgc
     3001 cctcgccgtc gccgatctgc acccgtccca gattgaactg atcatcgtgg ccacatcgac
     3061 gccagggcac atgtttccgt caacggcctg tctggtgcaa gactacctgg gcgcgtcgcg
     3121 ggccggcgcg ttcgatctgg gcgcggcctg ttcgggcttc gtctacgccc tgcaaatggc
     3181 gtcgcagtcc atcgccaccg gcgcggtgcg caatgccctt gtcatcggcg cggaaacaat
     3241 gtcgcgggtg gtcgattgga gcgaccgcgg cacgtgcgtc ctcttcggcg atggcgcggg
     3301 cgcggtcgtg ctcaagggtt ccagcatacc cggcggcgtc atggccacga cgctgcgctc
     3361 cgacggctcc ggggccgacc tgctgtcctt gcccgcgctc tatcacaacc ccgtacccat
     3421 cgccggggcc gaattcgccc acaacggcca ccacaagaac atcgtcgata tggacggccg
     3481 ccaggtctat cgcttcgcca ccaacgtcat cgcctcgtcg gtgcaggacg tgttgaagca
     3541 aagcgggctg acgctcgacg acatcgccct catcattccc caccaggcca acgtgcgtat
     3601 catcgagacg gccgcccgca agatgaagct ccccctggag aagttctatc tgaatctaga
     3661 tcataccggc aacacgtcgg ccgcgtccat ccccgtggcg ttatgcgacg ccgtctccga
     3721 aggacggctg gagccagacg acaacatcat ctttgtgggc ttcggcggcg ggctgacctg
     3781 gggggcgtcg ctggtgaagt gggacgtaac cccgccacag gtgtcgttca tcgaccacga
     3841 gtggaagcga gcgcgctatg tgatggcccg cgggcgctcg cggctgcggc ggctcagccg
     3901 gcgcgtcgag gccgccgtcg cccgctcatc caatggccac aagggcgacg actcggccga
     3961 gtagccgccg gcgcgtccct atttgagggc caggccaatg tggcgcagca gttcggcttc
     4021 gtcgggcggg ccgctgaagt ggacgcggcg gttaacgact acatggggca gcgccgtcac
     4081 ggagtagcgc tgcgccgcct ccgggaacag gtcggtcatg atcaggaagg cgcgcactcg
     4141 cggcgacgcc gcggccatgc caaagaccgt cttggccgcc accccgcccc actcatcctc
     4201 agccgcggtc agcagttcga tggcgatatc cgcgtcggcc ggcaagcggc tcaggcgaac
     4261 ccgcgtcgcc ggctccaacg tctgcccgcg aaaggcaacg gcctgaatag cggcgatgag
     4321 cgaggtcatt tgatagccca acggcaggcc gatgatgcgc aggccgtcat cgcgcgactc
     4381 cacagtcgtg ccacccatga cgccgataac cgggtagtag gggtaattct ggcggcgggg
     4441 aaaaagccgg tagctgagcc gtggcgagcg ttcggacagc gcctgggcca accgggtggc
     4501 ctcgcgctcg gccggctccc cctgctcgtc ggcccagacg tgcaggcaga ccgactccgg
     4561 caggttatcc aggaagccag gcagttgccg ccaggtctcg tcgtcgaagg gcacaccggg
     4621 cggcggggtg atgtcgctca ttgctcggcc ggctcctcgg acgcgcgtgg caggctgagg
     4681 ctcagggtcg tacccgcctc ggtgctgctg gcggcgacgt ggccgccgtg ggcttcggcc
     4741 accttgcggg ccacgaccag gcccaggtcg gtgccgcgcg tggcggcgcg tccttcgggg
     4801 ccaagctcta cccgctcgaa ggcgcgggcg atgacctcgg ccggcagccg ccggtcgggg
     4861 tcatgcactg acgtgcgccc ctggttgccg tcggcggcga cggcgatggt cagacgaccg
     4921 cgctcgcggg tgtacttttc ggcgtgactc agcaaactat agagggcgcg gccggccgcg
     4981 tcctcatcgg ccatgaccca cagcggctcc ggcggcgtgg cgagggtcag ttcaaccccc
     5041 ttgacactca tcagcccctc cacctgggcc aacgcctggc caaccagttc aaccaggtcg
     5101 caacggactg gttcgatctt cacctcgcca cgatccatcg catcgagggc gacgaggctc
     5161 tcgacgccat tgcgcaacga ggcggcatgg gcgatgatgg tcgtcaggta ctcgcgaatg
     5221 gccgggtcgc tctcggccgc gaggcttagc tccagcaact gcgcgtagcc ctggatgacg
     5281 gccaccggcg tgcgcagatc atgggcggcc atggccagca tgctcgattt acgctgctgc
     5341 aagcgctcca gtctggcatt ggcccgtgcc aactcgcgcc gcaggcggcg cacctcttcg
     5401 ctctcttccg gctccggcgc tgactctgat ggctgtttgt gtttcatgtg acggtactat
     5461 ccctgggcca aacgataaaa cggttaacga tactactcac tgccctggct ggcgtagcgc
     5521 tgcttccact cgtagagctt gttaagggcc tggaggggcg tcagggcgtt gacatccagc
     5581 gcctggagtt cgtccaaaaa ggggctggaa tcggggaaca aagcgatctg ctggctgcta
     5641 gacaggcggc taggctcgac ggccgtcgtt ggtgcgcggc gctccagctc gcgcaggatg
     5701 tcgttggcgc gcaagatgac gtcgcgcggc agcccggcca actgggcgac gtggatgccg
     5761 tagctgcggt cggccccgcc gggcacgata tggtgcagga aggtcacctc gccgccctcc
     5821 tcgctgacgg cgacgttgaa gttggccaca tcgggcagca ggccggccag cccgaccagt
     5881 tcatgatagt gcgtggcgaa gagcgtgcgc ggcttcaggc gggggtggtt gtgcaggaac
     5941 tcgacgatgg cccaggccag cgacagaccg tcgtaggtgc ttgtgccccg gccgatctcg
     6001 tctaacacca gcagcgaacg gtgggtggca tggttgagga tttcggccgt ctcgaccatc
     6061 tcgaccatga acgtcgaacg cccggcgtgc agttcgtcgt gggcgccgat gcgggtgaag
     6121 atgcgatcca ccagggcgat gtcggcggac gtggccggca cgaagctgcc cacctgggcc
     6181 atgagagcga tgagggccac ctggcgcaag taggtggact tgccgctcat attggggccg
     6241 gtgatgatct ggatgagatt ggagtcgtcg aagtgggtgt tgttgggcac gaagcgctcg
     6301 ccgcggcgcg cccccagccc gctgccctcg gccagcgccc cctcgacaac gggatggcgg
     6361 ccgttctcga tgtggatggc aatctcgccc gtctgcgacg ggtccgacag cgtgggccgg
     6421 atgtagtcgt tgagcgccgc gacctcagcc agcgccgcgg ccacgtctag ctcggccagc
     6481 gccgcggccg tccccagcag ccgcgcgcta tgttcgccga cgaagccgca caactcgctg
     6541 aatagccgcc gctcaatttg caggatgcgc tcctcggcat tgaggatgag cgtctcatac
     6601 tctttcagtt ccggcgtgat gtagcgttcg gcgttggtca gcgtctgctt acggatgtag
     6661 tcagagggca cgaggtgggt gttggcgtgg gtgacttcga tgtagtagcc gaagaccttg
     6721 ttgaacccga ccttgagcga ggcgatgccg ctgcgctgcc gctcgcgcgg ctccagcgag
     6781 gc
//
LOCUS       DAOSMQ010000122         6782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11295_length_6782_cov_4.345919, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000122 DAOSMQ010000000
VERSION     DAOSMQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11295_length_6782_cov_4.345919"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PK829_05555"
     CDS             complement(<1..360)
                     /locus_tag="PK829_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOU40713.1"
                     /translation="MRLGSHISTAGGAYRSFERAREASCESLLIFTKSNRQWAANLTT
                     LGRNKAEVYEELLRQINPDVRVRTHTEGITDANVNDFLAGADILIDCLDVAVAPALRA
                     KMFAAARAAGLYAATGAM"
     gene            complement(362..589)
                     /locus_tag="PK829_05560"
     CDS             complement(362..589)
                     /locus_tag="PK829_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40714.1"
                     /translation="MRGLVDRKGEQFAYLIGNVLFTMDGEPSGRLAGEMIVDLAGRPV
                     WRVVGDGVYALHDLETIGYLTEERREDEYQD"
     gene            complement(608..1390)
                     /locus_tag="PK829_05565"
     CDS             complement(608..1390)
                     /locus_tag="PK829_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU40715.1"
                     /translation="MSFRQMLPFLFLNIVVSAVVVLSILFWWDNRAGAAVAEATVAAT
                     LPPGVLATPNIAAPPSGTVAAEVAAADTAATGDETAVVHTVQAGDTLNRISQQYDVSV
                     DDIMAANGLTDPNVISVGQQLTIPVGGLPTATVPPPTEIAALPSPIPTEAVVTGQGEI
                     AITSVNTPGVLATEAVQIVNNGTALQSMRGWKVRDEDGNVYTFGEVGIFGEGAGILLH
                     SGAGVDSPLELFWGLTEPVWRSGETLTLWDAADQVVGTFVIP"
     gene            complement(1566..3563)
                     /locus_tag="PK829_05570"
     CDS             complement(1566..3563)
                     /locus_tag="PK829_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU40716.1"
                     /translation="MHTDFSLLATITMAVLFAFLCGFAARKLRLPSLVGYLIAGLLIS
                     PFTPGFVGDVSAASQLAEVGVIFMMFGVGLHFSLRDLWNVRQIAIPGAILQTTLSTLI
                     GLALGQLWGWSFEAGLVLGLSISVASTVVLLRGLTDHGLVSTPHGRVAIGWLVFEDLA
                     TIVILVLMPVLVGGSGNPLVSVGVALLKTAVFAVIMLVIGTRVLSWLLTQIAYTRSRE
                     LFILAVVALALGTSFAAFELFGVSLALGAFLAGVVIGESDVHHQVNAEAVPFRDFFSV
                     LFFVSVGMLVDPAVVMANIGKVVVLSLLVIVGKGVITLLLGFVLPSSARTMIVAAAGL
                     SQIGEFSFIVGQAGVGLGLLDGDQYGLILAAAVVSIVVNPLMFALIPRVERALRGVPW
                     LWRLIDRGGPTPEAFTEAIEGHVVIIGYGRVGSHIGSVLQQLGLPFLVVEQNMAYANE
                     IQARGIKTLFGDAANSEILLHTGLEHARALVVTAPNETTTELIVAAAHAIAPTLPIIA
                     RAATADRLPHLYHLGARHAINPEMEGGLEMLRYTLLSLGHGSNRIQPYLDAVRTETYE
                     GILPEEGGYPVLDQLLTATRGVEIAWELLNPGSPVIGHTLAEAGIRSRVGASVVAVLR
                     GGEVITNPDMSFVFQQDDLVGMIGRSGQLAEACYMLNAPGD"
     gene            3727..3987
                     /locus_tag="PK829_05575"
     CDS             3727..3987
                     /locus_tag="PK829_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40717.1"
                     /translation="MIVRATLTGKNQITIPAAIVKEMQLEPGMQLEFELGEQRTAVIR
                     PVLTRAERVKQLEGKWQHLFPPDSDPIGDLIRERELEDEEMD"
     gene            3990..4388
                     /locus_tag="PK829_05580"
     CDS             3990..4388
                     /locus_tag="PK829_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOU40718.1"
                     /translation="MLTHLLDTSAWIAHLQNETDAPTVANILSEPGNRVGISALSLVE
                     LHARLKTFGREEELNTILDDYREIFAQIVPVDEAIALLATKLRSNSSGRVPAIDAFIA
                     ATAAHRDAILVHRDPHFLALAGEVVRQQAL"
     gene            4671..6758
                     /gene="dnaG"
                     /locus_tag="PK829_05585"
     CDS             4671..6758
                     /gene="dnaG"
                     /locus_tag="PK829_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOU40719.1"
                     /translation="MTVTQEIKSRLDLVDVVQDAGVQLRKSGRNYAGFCPFHPNTKTP
                     AFYIFPESQTWRCFGACAEGGDVFSFVMKKNGWDFKETLTELAKRAGVTLEDHRPRDK
                     AHEAAEVRQAALLDAASHYFHQLLLHAPQAAAARRYVADRLLNDDTVATFRLGFALDS
                     WDALHTHFSAQGYSEDELLAAGLLTENEERHRRYDRFRNRLIIPIRDSDGRVVGFGAR
                     TLDPDGIPKYLNSPQTALFDKGHLIFGLDTAKRHIREARQVVLVEGYMDVMQAWQHGY
                     RNVVAQMGTSLTEAQLRLLQKLTKRFVLALDPDAAGAKATMRSLQLARETLDRDLEVS
                     FDARGLVQLEGRLRADIRIVTLPEGQDPDKLIRTDPAAWPRLLAEARPVVDYIIRVVA
                     AELPPGDAKAKSAAAAQVLPLIHDIAEPVERDHYLGVLSQTLGVDIKALRAAETRMKK
                     AGGKGQGRGSREPGPPPVTNARPAPGDQPPPYDAPPPYWDDTPPGDFDAPRGAIGQPG
                     SKIAGDLRRPPRLEENFLRQCLEYPGAIHHINRILSRQGEPEVSAADFERVEDRALFQ
                     VVLERAQPSRPEVATIEELCNSLDAVLAERVRSLMALPPSPGLKLDRLPDSLAQSVLD
                     WRLEKIRKRISELKQLRAGEQEPESAEELTRLYSDLLATSQPAMRRIHQAKDALSAQG
                     RRRAESNGSRRAR"
BASE COUNT         1242 a         2281 c         2188 g         1071 t
ORIGIN      
        1 catcgcgccg gtggcggcgt agaggccggc ggccctggcg gcggcgaaca tcttggcccg
       61 cagggcgggc gcgacggcga cgtccaggca gtcgatcagg atgtccgccc cggccaggaa
      121 gtcgttcacg ttggcgtcgg tgatgccctc ggtgtgcgtc cgcacgcgga cgtcggggtt
      181 gatctggcgg agcagttcct cgtagacctc ggccttgttt cggcccaggg tggtgaggtt
      241 ggccgcccac tggcggttgc tcttggtgaa gatgagcagc gactcgcacg aggcctcgcg
      301 cgcccgctca aaggaacggt aggccccgcc ggccgtgctg atatgggaac caagtcgcat
      361 attaatcctg atattcgtct tcgcgccgtt cttcagtcag gtagccgatg gtctccaggt
      421 cgtggagggc gtagacgcca tcgccgacga cacgccagac cggtcggccg gccagatcga
      481 cgatcatctc gcccgccagc cggccgctcg gctcgccgtc catcgtgaag aggacattgc
      541 caatgagata ggcgaactgt tcgcccttgc gatcgaccag gccccgcata gcccatccac
      601 ctccgtgcta gggaatgacg aacgtgccga cgacttgatc ggctgcgtcc cagagcgtca
      661 gcgtctcacc gctgcgccag accggctcgg tcagtcccca gaacaactcc agcgggctgt
      721 cgacgcccgc gccactgtgg agcaagatgc ccgcgccttc gccgaagatg cccacctcac
      781 cgaaggtgta gacgttgcca tcctcgtcgc gcaccttcca gccgcgcatc gactgaagcg
      841 ccgtcccgtt gttgacgatc tgcaccgcct cagtggccag cacgccgggg gtgttgacgc
      901 tggtgatggc gatttcgccc tggccggtga cgacggcctc ggtggggatg ggcgatggca
      961 gagcagcgat ctccgtcggt ggggggacgg tcgcggtcgg caggccgccc acggggatgg
     1021 tcagttgctg cccaacggag atgacgttgg ggtcggtcag gccgttggcg gccatgatgt
     1081 cgtccacact gacgtcgtat tgctggctga tgcggttgag cgtgtcgccg gcctgcacgg
     1141 tgtggacgac ggccgtctcg tcgccggtgg cggccgtgtc ggcggcagcg acctcggccg
     1201 ccaccgtgcc cgacggcggc gcggcgatgt tgggcgtggc cagcacgccc ggcggcagtg
     1261 ttgcggccac tgtcgcttcg gccacagccg cgccggcgcg gttgtcccac cagaagagga
     1321 tgctcagcac gacgacggcc gagacgacga tattcagaaa caggaagggc agcatctgac
     1381 ggaaggacat acgcctatta taacctgagt tcggcattag gagggagaca acccccctgt
     1441 tggccagggc ttttctaaag tcaccccggc acactcacct gtcaggtcat tggccaagcc
     1501 cggctctgtt ggccaaagga catgggtcaa atcccaatcc ggcaacagct gctcaccatc
     1561 aggccctagt cgcccggcgc gttgagcata taacacgcct ctgccaactg cccactgcgg
     1621 ccaatcatcc ctaccaggtc atcttgctgg aacacgaagc tcatgtccgg gttggtaatc
     1681 acctcgccgc cgcgcaggac ggccacgacc gacgcgccca cccgcgagcg gatgccggcc
     1741 tcggccaggg tgtggccgat gaccgggctg ccggggttca gcagctccca ggcgatctcc
     1801 acaccgcgcg tggccgtcag cagttggtcg agcacggggt agccgccctc ctccggcaag
     1861 atgccctcgt aggtttctgt gcgcacggcg tcgaggtagg gctggatgcg gttgctgccg
     1921 tgccccagcg acagcagcgt gtagcgcagc atctccagcc cgccttccat ctccgggttg
     1981 atagcgtggc gcgcgccgag gtggtagagg tggggcaggc ggtcggccgt cgccgcgcgg
     2041 gcgatgatgg gcagcgtcgg ggcgatggcg tgggccgcgg cgacgatcag ttccgtcgtc
     2101 gtctcgttag gcgcggtgac gaccagcgcg cgagcatgtt ccagcccggt gtggagcaga
     2161 atctcggagt tggccgcgtc gccgaacagc gtcttgatgc cccgcgcctg aatctcgttg
     2221 gcgtaggcca tgttctgctc gaccaccagg aagggcaggc ccaactgctg gagcacgctg
     2281 ccgatatggc tgccgacgcg gccgtagccg atgatgacca catgcccctc gatggcttcg
     2341 gtgaatgcct ccggcgtcgg cccgccgcga tctatcagcc gccatagcca gggcacaccc
     2401 cgtaacgccc gttccacgcg cgggatcagg gcgaacatca gcgggttgac gacgatggag
     2461 accactgccg cggccaggat cagcccgtac tggtcgccgt ccagcagccc caaccccacg
     2521 ccggcctgcc cgacaatgaa cgagaactcg ccgatctggc tcaggccggc ggcggcgacg
     2581 atcattgtcc gcgccgacga gggcaggacg aaccccagca gcagcgtgat cacccccttg
     2641 ccgacgatga ccagcagcga cagaaccacg accttgccga tgttggccat caccaccgcc
     2701 ggatcgacca gcatgcccac ggagacgaag aacagcaccg agaagaagtc gcggaagggc
     2761 acggcctcgg cgttgacctg gtggtggacg tccgactcgc cgatgaccac gccagccagg
     2821 aacgccccca gcgccagcga cacgccgaac agctcgaagg cggcgaagga cgtgcccagg
     2881 gccagggcca cgacggccag gatgaacagc tcgcgcgagc gagtgtaggc gatttgcgtc
     2941 agcagccacg acagcacgcg cgtgccgatc accagcatga tgaccgcaaa tacggccgtc
     3001 ttcagcagcg ccacgccgac gctgaccagt gggttgccgc tgccgcccac cagcacgggc
     3061 atgagcacga ggatgacgat cgtggccagg tcttcgaaga cgagccagcc gatggccacg
     3121 cggccgtggg gcgtgctgac caggccgtga tcggtcaggc cgcgcagcag caccaccgtg
     3181 ctggcgacgg agatcgacag gcccagcacc agcccggcct caaacgacca gccccacagt
     3241 tggccgaggg ccagaccgat gagcgtggac agcgtcgttt gcaggatcgc cccggggatg
     3301 gcgatctggc ggacgttcca caggtcgcgc agcgagaagt ggaggccgac gccgaacatc
     3361 atgaagatga cgccgacctc ggccagttgg ctggcggcgc tgacgtcgcc gacgaagccg
     3421 ggggtgaagg ggctgatgag tagcccggca atgagatagc cgacgagcga cggcagacgc
     3481 agcttgcggg ccgcgaagcc gcacaggaag gcgaacagca ctgccatcgt aatggtggcc
     3541 aggaggctga aatcggtgtg catcgtccta ttctacctcc aaagctggcg atcctggaac
     3601 ctgagtttct ccgaggagac ctggtttctg gtcccgcttt ccttgaattg cgcatctcct
     3661 tgattgactt ccttccttga tgtatacttc tatcaaggga tattcttgct ggcgaggtgc
     3721 agttttatga tcgtacgagc aacactgacc ggaaaaaacc aaatcaccat tccggcggcc
     3781 atcgtgaagg agatgcaact ggagccgggc atgcagctgg agttcgagtt gggcgagcag
     3841 cgaacggcag tcatacggcc agtgctcaca cgtgcggagc gcgtcaaaca attagaaggc
     3901 aagtggcaac atttgttccc gcccgactct gatccgatcg gcgatctgat tcgcgagcgg
     3961 gaacttgaag acgaggagat ggactaggcg tgttgactca cttgctggac acgtccgcct
     4021 ggattgctca cttgcagaac gagacggatg ctcccaccgt tgccaacatc ctgtctgagc
     4081 ctggcaatcg ggttggcatt tctgcactca gcttggtcga gttgcacgcc cgcctaaaaa
     4141 cgttcggccg cgaagaagaa ctcaatacca tactggacga ctaccgtgag attttcgctc
     4201 agattgtgcc agtagacgag gcgatagcgc tgctggcgac gaaactccgc tcaaactcgt
     4261 cgggacgcgt tccggccatt gacgccttca tcgccgccac ggccgcccac cgcgacgcca
     4321 tcctcgtcca ccgcgacccg cattttctgg ccctggcggg tgaagtagtg cggcaacagg
     4381 ctctttagtc tgacgccgtt tctcccttct ggggaatcag cacgccttgc tggtagagag
     4441 tgtcagggca aaagtcctgg ccgttccgcc actggacgct gcccaggaat ggccgcacgg
     4501 agttgaaggc gcgcaggtcc ttgagttcgt cgcgattaga acatccaccc gacttgagat
     4561 ataatcccct tcgccgacaa ccgcgactga cataaactcg ccgctctcct tgtcctggcc
     4621 ggcgggttcc gaagctgaat cgcctgtgca ttgcgcgacc gctcgctgac atgactgtaa
     4681 cgcaagagat taaaagccgg ctcgatctcg tcgacgtcgt ccaggacgcc ggcgtgcaac
     4741 tgcgcaaatc gggacgcaac tacgccggct tctgcccctt ccaccccaac accaagacgc
     4801 ccgccttcta tatcttcccc gaaagccaga cgtggcgctg ctttggggcc tgcgccgagg
     4861 gcggcgatgt cttctccttc gtcatgaaga agaacggctg ggacttcaag gagacgctga
     4921 ccgagctggc caagcgcgcc ggcgtcactc tggaagacca ccgcccgcgc gacaaggccc
     4981 acgaagcggc cgaagttcgc caggccgccc tgctcgacgc cgcctcgcac tacttccacc
     5041 aactcctcct ccacgcgccg caggccgcgg ccgcccgccg ctacgtcgcc gaccggctgc
     5101 tcaacgacga taccgtggcc accttccggc tcggcttcgc cctcgatagc tgggacgccc
     5161 tgcacaccca cttcagcgcc cagggctaca gcgaggacga gttgttggcc gccggcctgc
     5221 tgaccgagaa cgaggaacgc caccgccgct acgaccgctt ccgcaaccgc ctgatcatcc
     5281 ccatccgcga cagcgacggc cgtgtcgtcg gcttcggcgc gcgcacgctc gaccccgacg
     5341 gcatccccaa gtacctcaac agcccccaga cggcgctgtt cgacaagggc catctcatct
     5401 tcgggctgga cacggccaag cgccacatcc gcgaggcgcg ccaggtcgtc ctcgtcgagg
     5461 gctacatgga cgtgatgcag gcctggcagc acggctatcg caacgtcgtc gcccagatgg
     5521 gcacgagcct gaccgaggcg caactgcgcc tgctgcaaaa gctgaccaag cgcttcgtgc
     5581 tggccctcga tcccgacgcc gccggggcca aggcgaccat gcgcagcctg caactggccc
     5641 gcgagaccct cgaccgtgat ctggaagtga gctttgacgc ccgcggcctg gtgcaattgg
     5701 aggggcggtt gcgggccgac attcgcattg tcaccctgcc ggaggggcaa gaccccgaca
     5761 agctcatccg caccgacccg gcggcctggc cgcggctgct ggccgaggcg cggccggtgg
     5821 ttgactacat catccgcgtt gtcgccgccg agttgccgcc gggcgacgcc aaggccaaga
     5881 gcgctgccgc cgcccaggtg ttgccgctca tccacgacat cgccgagccg gtggagcgcg
     5941 accactacct gggcgtgctc agccagacgc tgggcgtgga catcaaggcg ctgcgggcgg
     6001 ccgagacgcg catgaagaag gcgggcggga agggccaggg gcgaggaagc cgggagccgg
     6061 ggccgccgcc ggtcaccaac gcgcggccag caccgggcga ccagccgcca ccctacgacg
     6121 cgccgccgcc ctattgggac gacaccccgc cgggcgactt cgacgccccc cgcggcgcga
     6181 ttggccagcc gggcagcaag atcgccggcg acctgcgccg gccgccgcgc ctggaggaga
     6241 acttcctgcg ccaatgcctg gaataccccg gcgcgatcca ccacatcaac cgcatcctca
     6301 gccgccaggg cgagccagag gttagcgccg ccgacttcga gcgagtcgag gatcgcgccc
     6361 tcttccaggt cgtcctcgaa cgcgcccagc ccagccgccc ggaggttgcc acgattgagg
     6421 aattgtgcaa tagtctagac gcggtgttgg cagagcgggt gcggagtctc atggccctgc
     6481 ccccgtcgcc cgggctgaag ttggatcggc tacccgactc cctggcgcaa tcggtgctcg
     6541 attggcggtt ggaaaaaatc cgcaagcgga tatccgaact gaagcagttg cgcgctggcg
     6601 aacaggagcc ggaatcggct gaggagctaa cccggctcta cagcgatcta ttggccacat
     6661 cgcaaccggc gatgcggcgc atccatcagg ccaaagatgc gctgtcggcc caggggcggc
     6721 gacgcgccga aagcaacggc tcgcgccgcg cgcgctgagc gacgcccaca ccggcccatt
     6781 gc
//
LOCUS       DAOSMQ010000123         6755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11359_length_6755_cov_3.529851, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000123 DAOSMQ010000000
VERSION     DAOSMQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11359_length_6755_cov_3.529851"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PK829_05590"
     CDS             complement(<1..744)
                     /locus_tag="PK829_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40720.1"
                     /translation="MRPRGNLPHRSLTLILAIIALLLAALYVAGLVVDWPGWLRGSNW
                     VWVRRVPALGARLWLLSAALAVGLALAAVAARGGPWSRRRTALLLAAAVVLTPAVQVA
                     VAAQHRAQPLSAVVLSSGGFWQEGARIDDPLRFAREHAARMPAYADIHVRTQPPGWPL
                     AYWAAAQVWARFPAAAAAVGLRLLRYDCLAAEMQGLSAAQLAAGSLRIAILALTGLGA
                     LPLYAIGRRFFAPAAARLAAVAYAYLPALL"
     gene            789..2336
                     /locus_tag="PK829_05595"
     CDS             789..2336
                     /locus_tag="PK829_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40721.1"
                     /translation="MTAARPETAVLARWARLAPWAALLLFAAVVIRAAWLSDDAYITF
                     RVVDNAVAGLGLTWNPAERVQVYTHPLWLFTLLTGRLATGELYYTAIWLSVALSVAAA
                     ALVARRRPPLVAALGVAVLALSRAFVDFSTSGLENALSHLLLAAFLTLWFGRPPDVRG
                     FGDRRLFALSLLAGLLALNRSDALLLALPAVLYALWRQRSGRALLGVALGFLPLALWT
                     AFSLFYYGFPLPNTAYAKLATGIPAPVLALQGWRYLLNSLRWDPLTLTAIAAAEALGL
                     ALRRAAAVAIAAGIALYLLYVVRIGGDFMSGRFLSLPLLAAVMLLIELSPSRYTKISA
                     LALSLLALLALIGHAPVWLAGNPTGDAARRDGDIADERAYYTPNTGLFAARVPHPWAE
                     QGLVLRAQGPAVVEHGNVGFLGYYAGPAVHIIDRNGLTDPLLARLPVRDPANWRIGHF
                     RRAVPAGYAETLRGGVNVIADPHLAAYYEPLRLATRGPLWNRDRLAAIWRLNTGAYDA
                     LLPEVWP"
     gene            2333..>2592
                     /locus_tag="PK829_05600"
     CDS             2333..>2592
                     /locus_tag="PK829_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40722.1"
                     /translation="MSEVVVIGNAGIDTNVYLYGADVDFSVEANFSDNRDVVGQAGSY
                     SARGYAALGRATAFIGHVGDDHNGRRVRPRQRVENVVGQRFHR"
     assembly_gap    2593..2692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2693..3264)
                     /locus_tag="PK829_05605"
     CDS             complement(<2693..3264)
                     /locus_tag="PK829_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40723.1"
                     /translation="MHRRVVLCLCLIGLLALSACEAFRLTPVEPTPAPVHDSPFGQMG
                     RYRTQSGRFEVDFPAAWNEAEGDDRRCQDAPKCLISRDSEFMLVREQTMTRGPDSLAA
                     ELDAVVAGFEDTTADAVFLSREPFTTSAGLPGEILRYSIEQGRVSVKELWAADGDQAV
                     SVAFITWNEGFAAMEPLADYVFDTLTRTDAA"
     gene            3521..4846
                     /locus_tag="PK829_05610"
     CDS             3521..4846
                     /locus_tag="PK829_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU40724.1"
                     /translation="MTKTIILLISLLLLVACGAPNTEIRPTPIDLAGGQGGGAADEGG
                     GVGHTVSLRLWAPNDDARNAAYQALIVAYTNTHPDVSIQLETFDPTAYAQTLQSALAS
                     GGAADIIQLPGSACPWAAAGVLGEVPAAVLNPTDAATRVAVAGSLCDNRLYSLPQTVT
                     LPAAAALVDRAAAEAAGLTGIAGGWATWDELLADARALAVVQDGVMTRAGFHFIDPEA
                     LPALFFGLILQNGGQPATNGIVTANTPQGAAALALLKRFVDEGAIDPQRFNDATDPLA
                     RSALEGRSAMSVAPPGVAAQWNAAHPDAPALYLPLPWAGAPAVAAPGWALAVTNLSAA
                     PEVAWDFARFVALEPANAAQWSQAAGALPALLANREGEAVAQLAAAQPALAPLLPLLP
                     AATDAGLGAGHDRLWREVAYPRLLAYLQGAATAEETLTGIDAAASAPAP"
     gene            complement(4905..>6755)
                     /locus_tag="PK829_05615"
     CDS             complement(4905..>6755)
                     /locus_tag="PK829_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40725.1"
                     /translation="RLVGLALMVVFLFLLVAPAGQAQDADAAGLRPGETVVYRQTIPI
                     NIVFVGFDREDIAVPDVRAQLPAGYTPKVRYPLFYGLPGRNMGLRFDFEYKPIFTDKA
                     FEDAFFGYLASIAAPGAPTVFQQLYNGNANNVLDVSGNVGYIDAPSAEQWLMTAGEQW
                     LGINPDRSYTVYYINWYDRPDFQFHVYTKTDSPDPDTGYNFGQLRASRKMVAWGGSHG
                     RTWFYDLSAGPDSWTGNYDVDHADVDGDGEADYRLPVIWEYAANGYRHPAALSSDLGL
                     VTRYVAINLLFTTSPLYDPMVAAPGPGGKRVADIEIFELHRLSQGVNWIDGPYIQAEY
                     ERFQPYYDWEVVVDQNRSTPGVRRAVRIGGGLSTQPDCWTEYGTPFAEYFCYFDENYS
                     HFVPEYGADDNVAAVFGFNGALKNLGGVPLGFADDNWRDGTQSYVFMFDGPEIRAAGY
                     GFTTTTIHELGHYYGLSHPHDGYDSATGVDYGASGDFYFANLGDEVGSMMSYIDVGWK
                     FGHFDEDNMYRYEFAGYLNLCNELLAGILAQPGHGQVNDLVAAGREHARMAERAFKQW
                     NYLEAVTQARMAYEAIGTAAQQLGLPVVAADAAPQMPINGSVPRFIDPIR"
BASE COUNT         1036 a         2411 c         2178 g         1030 t
ORIGIN      
        1 cagcagcgcc ggcaggtagg cataggccac ggccgccagc cgcgccgccg ccggcgcgaa
       61 gaagcgccgc ccgatggcat agaggggcag cgcgcccagg cccgtcaggg ccagaatggc
      121 tatgcgtaaa ctgccggccg ccagttgggc cgccgatagc ccctgcatct ccgcagccaa
      181 acagtcatac cgcagcagcc gcaggccaac ggcagcagcg gccgcgggga agcgcgccca
      241 gacctgcgcc gccgcccagt aggccagcgg ccagcccggc ggctgcgtgc gcacgtgaat
      301 atcggcatag gccggcatcc gcgcggcgtg ctcgcgggcg aaacgcagcg gatcgtcgat
      361 gcgcgcgccc tcctgccaga agccgccgga gctgagcacg acggcgctga gcggctgcgc
      421 ccggtgctgg gcggccacgg cgacctgcac cgccggcgtc aggacgacgg cggcggccag
      481 cagcagcgcc gtccgccgcc gcgaccacgg cccgccgcgc gcggccacgg ccgccagcgc
      541 caaccctacg gccagcgctg ccgacaacag ccacaggcgc gcccccagcg ccggcacgcg
      601 ccgcacccag acccagttgc tgccgcgcag ccagcccggc caatcgacga ccagcccggc
      661 gacgtagagc gccgccaaca gcagtgctat aatcgccagg atgagcgtga gagaacggtg
      721 ggggagattg ccgcgtggcc tcatggcgag tccattctag cacagcaccg gccggggccg
      781 aaccagcaat gacagccgcg cgtccggaga cggccgtcct tgcccggtgg gcacgtctgg
      841 ctccgtgggc cgcgctcctg ctcttcgccg ccgtcgtcat acgcgcggcc tggctgagcg
      901 atgacgccta catcaccttt cgcgttgtcg ataatgccgt cgccgggctg gggctgacct
      961 ggaacccggc cgagcgcgta caggtctaca cccacccgtt gtggctgttc acgttgctga
     1021 ccggtcgcct ggccaccggt gaactgtact acacggccat ctggctgtcc gtggccctgt
     1081 cggtggccgc ggcggcgctg gtcgcccgcc ggcgaccgcc cctcgtcgcc gcgctgggcg
     1141 tggccgtgct ggcgctgtcg cgcgccttcg tcgatttctc cacctccggc ctggagaacg
     1201 ccctcagcca cctgttgctg gccgcgtttc tgacgctgtg gttcggccgt ccgccagatg
     1261 tccgcggctt tggcgaccgc cgcctgtttg cgctgtcgtt gttggccgga ctgctggccc
     1321 tcaaccgcag cgacgcgctc ttgctggcgc tgccggcggt gctctatgcg ctctggcggc
     1381 aacggtcggg gcgggcgctg ctcggggtgg cgctgggctt cctgccgctg gcgctatgga
     1441 cggcattctc gctgttctac tatggtttcc cccttcccaa cactgcctat gcgaaattgg
     1501 ccaccggcat ccccgcccct gtcctggccc tgcagggctg gcgctatctg ctcaactcgc
     1561 tgcgctggga cccgctgacg ctgacggcca tcgccgcggc cgaggccctg ggtctggcct
     1621 tgcgccgggc cgcggccgta gccatcgccg ctggcatcgc tctctacctg ctctacgttg
     1681 tccgcatcgg cggcgacttc atgagcggac gttttctatc tctgccgctt ctggcagcgg
     1741 tcatgctgct catcgaactg tctccgtctc gctacacgaa aatcagcgcg ctcgctctga
     1801 gtctgctggc cctcctcgcg ctcatcggcc acgcgcccgt ctggctggcc ggcaatccca
     1861 ccggcgacgc cgcccgccgc gatggcgaca tcgccgacga acgcgcctac tacacgccca
     1921 acaccggcct cttcgccgct cgcgtccccc acccctgggc cgagcagggt cttgtcctgc
     1981 gcgcccaggg gccggccgtc gtcgagcatg gcaacgtcgg cttcctgggc tactacgccg
     2041 gaccggccgt ccatatcatc gaccgcaacg gcctgaccga cccgctgctg gcccgcctac
     2101 cggtgcgcga cccggccaac tggcgcatcg gccactttcg ccgcgccgtg cctgctggct
     2161 acgccgagac gctgcgcggt ggcgtgaacg tcatcgccga cccccatctg gccgcctact
     2221 atgagccgct gcgcctggcc acgcgcgggc cgctgtggaa tcgcgaccgg ctggcggcca
     2281 tctggcggct caatacgggc gcctatgacg cccttctccc ggaggtctgg ccatgagcga
     2341 ggtcgtggtc atcggcaacg ccggcatcga caccaacgtc tatctctacg gcgcggacgt
     2401 ggacttcagc gtcgaggcca acttcagcga taaccgcgac gtggtgggcc aggccggcag
     2461 ctacagcgcc cgcggctacg cggccctcgg ccgggccacc gcctttatcg gccacgtcgg
     2521 cgacgaccac aacggccgcc gcgtccgtcc gcgtcagcgt gtcgaaaacg tagtcggcca
     2581 acggttccat cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccgcgtc
     2701 cgtccgcgtc agcgtgtcga aaacgtagtc ggccaacggt tccatcgccg cgaagccctc
     2761 gttccaggtg atgaaggcca ccgacacagc ctggtcgccg tcggccgccc acaattcctt
     2821 gacgctgacg cgcccctgct caatggagta gcgcagaatt tcgcccggca agccggcaga
     2881 ggtagtgaac ggctcacgcg agaggaacac ggcgtcagcg gtcgtgtcct cgaagccggc
     2941 cacgacggca tcaagttcag ccgccagcga gtccgggccg cgggtcatcg tctgttcgcg
     3001 cacgagcatg aattccgagt cgcggctgat gaggcacttg ggcgcgtcct ggcagcggcg
     3061 gtcgtccccc tcagcttcgt tccacgccgc cgggaaatcg acctcgaagc ggccagactg
     3121 ggtgcgatag cggcccatct ggccgaaggg ggagtcgtga acgggcgcgg gcgtcggctc
     3181 caccggcgtc agccggaagg cctcgcacgc agacagggcc agcaagccaa tcagacacag
     3241 gcacaggaca actcgtcgat gcataggctc cataagcctc cattctagcc gcccaccccg
     3301 cgcaatgcat cctcagcgcc ccgggcgcac cgacctaaca ggtggatggc cacgactggc
     3361 cgctcacctg tcaggtcaat ggtcgctaga cggccctggc aaagcccggc ggcgccatct
     3421 cagcgcctgg cactttctta ctcgccctca agaaggtgcg acgcgccagc ccttgcccca
     3481 aacggcttca tgctctatcg tggacactgg agagaagcca atgaccaaga ccattatcct
     3541 gctcatcagc ctgctgctgc tcgtcgcctg cggcgcgcca aacacggaga ttcgccccac
     3601 ccccatcgac ctcgccggcg gccagggcgg cggcgcggcc gacgaaggcg gcggcgtcgg
     3661 ccacaccgtg tcgttgcgcc tgtgggcgcc caacgacgat gcccgcaacg ccgcctacca
     3721 ggccctcatc gtcgcctata cgaacaccca cccggacgtc agcatccagc tggagacgtt
     3781 cgaccccact gcctacgccc agaccctcca atcggccctg gcctctggcg gcgcggccga
     3841 catcatccag ttgcccggct cggcttgccc ctgggccgcg gccggcgtcc tgggcgaagt
     3901 tcccgccgcc gtgctcaacc cgaccgacgc cgccacgcgc gtggccgtgg ctggcagcct
     3961 ttgcgataac cggctctata gcctgccgca gacggtgacg ctgcccgccg ccgcagcgct
     4021 ggttgaccgg gccgcagccg aagctgccgg tctgaccggc attgccggcg gctgggccac
     4081 gtgggacgaa ctactggccg acgcgcgcgc cctggccgtg gtccaggatg gggtcatgac
     4141 ccgcgccggg ttccacttca tcgatcccga agcgttgccg gcgctcttct tcgggctgat
     4201 cctgcaaaac ggggggcagc cggcgaccaa cggcatcgtg acggccaaca cccctcaggg
     4261 cgcggccgcg ctggcgctgc tgaagcgctt cgtcgacgag ggcgcaatcg acccgcagcg
     4321 cttcaacgac gccaccgacc cgctggcccg gtcggcgctg gaggggcgca gcgcgatgag
     4381 tgtcgcgccg ccgggggtgg ccgcccaatg gaacgccgcc cacccggacg cgccggcgct
     4441 ctatctgccg ctgccctggg ccggcgcgcc ggcggtcgcc gcgcccggct gggcgctggc
     4501 cgtcaccaac ctgagcgccg ccccggaggt ggcctgggac ttcgcccgct tcgtcgccct
     4561 ggaaccggcc aatgctgcgc agtggagcca ggcggccggc gcgctgccgg cgctgctggc
     4621 caaccgcgag ggcgaggccg tcgcccaact ggccgcggcg cagcccgcgc tggcgccgct
     4681 gctccccctg ctgcccgcgg ccaccgacgc cggcctgggc gccggccacg accgcctctg
     4741 gcgcgaggtc gcctatccgc gcctgctggc ctatctgcaa ggcgcggcca cggccgagga
     4801 aacgctgacg ggcattgacg ccgctgccag cgcgcccgcg ccttaggggc gagctttcct
     4861 cgaccgtggc cgcaacgggc aagcgttggg cttgccgccg gcgattaccg gatcgggtcg
     4921 ataaaccgcg gcacggaacc gttgatgggc atctgcgggg ccgcgtcggc ggccacaacc
     4981 ggcagaccca actgctgcgc cgccgtgcca atggcctcat aggccatccg cgcctgcgtc
     5041 acggcctcca ggtagttcca ctgcttgaaa gcccgctcgg ccatgcgcgc gtgctcgcgg
     5101 ccggcggcca ccagatcatt cacctggccg tggcctggct gggccagaat gccggccagc
     5161 agttcgttgc acaggttcag gtagccagcg aactcgtagc gatacatgtt gtcctcgtcg
     5221 aagtggccga acttccagcc cacatcgatg taggacatca tcgagccaac ttcgtcgccc
     5281 aggttggcga agtagaagtc gccgctcgcg ccgtagtcca cgcccgtggc cgagtcgtaa
     5341 ccatcgtgcg ggtgcgacag gccatagtag tggcccagtt cgtggatggt cgtcgtcgtg
     5401 aagccatagc cagcggcgcg gatttccggg ccatcgaaca tgaagacata gctctgcgtg
     5461 ccatcgcgcc agttgtcgtc ggcaaagccc agcggcacgc cacccaggtt cttcaacgcg
     5521 ccgttgaagc caaagaccgc ggcgacgtta tcgtccgcgc catattcggg cacaaaatgg
     5581 ctgtagttct catcgaagta gcagaagtac tcggcgaagg gtgtgccata ttcagtccag
     5641 cagtccggct gcgtgctcag accgccgcca atgcgcaccg cgcgccgcac gcccggcgtc
     5701 gagcggttct gatcgacgac aacctcccag tcatagtagg gctggaagcg ctcgtattcg
     5761 gcctggatgt acggcccatc gatccagttg acgccctgac tcaggcggtg cagttcgaag
     5821 atctcaatgt cggccacgcg cttgccgccg gggccggggg cggccaccat cgggtcataa
     5881 agcggcgagg tggtgaacag caagttaatg gccacgtagc gcgtcaccag ccccaggtcg
     5941 gacgacagcg ccgctgggtg gcgatagccg ttggcggcgt actcccagat cacggggagg
     6001 cggtaatcgg cctcaccgtc gccgtccaca tcggcatggt ccacgtcgta gttgcccgtc
     6061 caggagtccg ggccggccga gaggtcatag aaccaggtgc ggccgtggct gccaccccag
     6121 gcgaccatct tgcgcgaggc gcgcaattgg ccgaagttgt agcctgtgtc ggggtcgggg
     6181 ctgtcggtct tggtgtagac gtggaactgg aagtcgggcc ggtcatacca attgatgtag
     6241 tacaccgtgt agctgcggtc agggttgatg cccaaccact gctcgccggc ggtcatcaac
     6301 cactgttcgg ccgacggcgc gtcgatgtag ccgacgttgc cgctcacgtc gaggacgtta
     6361 ttggcgttgc cattgtacag ttgctggaac actgtcggcg cgcccggagc ggcgatagag
     6421 gccagatagc caaagaaggc atcttcgaag gccttatccg tgaagatagg cttgtactcg
     6481 aagtcgaagc gcagccccat attgcggccg ggcaggccgt agaagagcgg gtagcgcacc
     6541 ttgggggtgt agccggccgg caactgcgcg cgcacgtccg gcacggcgat gtcttcgcgg
     6601 tcgaagccga cgaagacgat gttgatcgga attgtctgtc gatagaccac ggtttcgccc
     6661 ggcctaagcc cggccgcgtc cgcatcctgg gcctggcccg ccggagccac cagcaggaac
     6721 aagaagacca ccatgagagc cagccccacc agccg
//
LOCUS       DAOSMQ010000124         6746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11383_length_6746_cov_4.723958, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000124 DAOSMQ010000000
VERSION     DAOSMQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11383_length_6746_cov_4.723958"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..426)
                     /locus_tag="PK829_05620"
     CDS             complement(136..426)
                     /locus_tag="PK829_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOU40726.1"
                     /translation="MSSPPTSCLEATVYGLVQGVYFRQYTWQEARRLGLVGWVANQSD
                     GTVRVVVEGPAEALRQLLTFLHHGSPSARVERVESVWKDASGAFTDFRVRGV"
     gene            complement(510..1556)
                     /locus_tag="PK829_05625"
     CDS             complement(510..1556)
                     /locus_tag="PK829_05625"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOU40727.1"
                     /translation="MPDLYTQLLFPVFKRFDPETAHERTLAALALAQATPAGRLALRR
                     MAGRLPALPVLIGPLRFPNPLGMAAGFDKDARVVVGLAALGFGHIEVGTLTPRPQAGN
                     PRPRIFRLPADEALINRMGFPNGGVAAAGPRLQALSARPRGYVLGVSLGKQKETPLEE
                     AAADYVAVMRAVYNHADYLAVNISSPNTPGLRALQGGRYLEGLLAALMAESRILATSA
                     GGALKPLWVKIAPDLGWDELDEVLTALTAAGVAGVIATNTTLAREGLRDRRQAEAGGL
                     SGRPLRARSTELIAAIYRRTDGKLPIIGVGGIASASDAREKLDAGASVVQLYSSLVYA
                     GPRLPGRILRGLAG"
     gene            complement(1549..3396)
                     /locus_tag="PK829_05630"
     CDS             complement(1549..3396)
                     /locus_tag="PK829_05630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOU40728.1"
                     /translation="MTHWNRAWRARAWQQLSADWDIVVVGGGITGAGILHEAARLGLR
                     ALLVEQRDFAWGTSSRSSKLVHGGLRYLRTGQVGLTRASVQGREQLLAEGAGLVHPLG
                     FLLAQYEGDGAGQLVYGAGLAVYDLMALQWSHRYYSPADFQLLAPYISPTGLRGGFQY
                     NDAQTDDARLVLRLIREAVDAADDAERQVLALNYVRAEELLRDDAGEVAGVRLVDTSG
                     DEAGRLPVDVRARAVINATGAWADFLRGQVGGEARLRPLRGSHLVFPAWRFPVAQAIS
                     FMHPVDGRPVFAFPWEGVTLLGTTDVDCPPPLDADPSITGEEVAYLMAAVTAQFPALG
                     LTLDDISATFAGIRPVIGSGKVDPSEESRDHVVWQEAGLLTVTGGKLTTFRQVGRQAL
                     EQVCARVDDGAAWLARLHDDAPLLWMATGATVSGDEAAPDSLPGGERLADEVRCRLLG
                     RYGRAATGLVAAAGPGDLEAIPGTTALWAELRWAARAEGVAHLDDLLLRRVRLGLLLP
                     RGGGDLMPHIRAIAQPELGWDDARWMDEERRYRQLVAAAYALPDRRDISDWNVLLATS
                     QADRERAAVARQARGRARQRRGAAALLVAALLLLIVGLLRRDRQQAGHA"
     gene            complement(3393..5033)
                     /locus_tag="PK829_05635"
     CDS             complement(3393..5033)
                     /locus_tag="PK829_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU40729.1"
                     /translation="MRRWNGWGDDTIPYLLPATAPAFLEERLGPGTPPRDTSFAEVAA
                     TVPPGRLPSHPLISAEAADRLRHARGQSLPDWVALRSGRVAAFPDGVAYPLTESDVAG
                     LLSFAAAVGARLIPYGGGTSVVGHINPEPGDAPALTVSLRRMNALLRFDSVSHLATFG
                     AGVAGPELEAQLRARGFTLGHFPQSFEYSTLGGWVATRSSGQQSLGYGRIESLFAGGT
                     LLSPAGVLRLPSFPASAAGPDLRQLVLGSEGRLGILTEATVRVTPLPEHEEFHAVFFP
                     DFAGATTATRQIVQGRWPLSLLRLSTPRETQTTLALAGHERLIGLLETLLSVRGVGGE
                     KCMLLCGFTGRAATIRDARDQVLDICRQQGGVSAGRRFGAQWIEGRFRTPYLRNTLWE
                     RGYAVDTLETATTWDNVPRLLGQIEDALGRALDDRGERVHIFTHLSHVYPQGASIYTT
                     YLFRLAAGPDETLDRWRALKGAASRAIVAGGGTISHQHGVGRDHAPYLAAEKGALGLG
                     ALAAAARCFDPAGHMNPGVLLAADGAGGQPEGRRGGRP"
     gene            complement(5035..5727)
                     /gene="fadR"
                     /locus_tag="PK829_05640"
     CDS             complement(5035..5727)
                     /gene="fadR"
                     /locus_tag="PK829_05640"
                     /inference="COORDINATES: protein motif:HMM:NF003444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HOU40730.1"
                     /translation="MTEWLAPVRPRAHAESVLLAAILDGTFPPGTTLPGERTLAAQLG
                     VTRPTLREALQRLARDGWLTVAHGKLTLVNNYWEEGGLNVLGHLVEHPDYLPPDFIAQ
                     LLEVRLHLAPAYTRAAVARDAALLTTFLDGAAVLETPADFARFDWQLHHLLTVRSGNP
                     IYTLILNGFRGFYEDIARRYFATVEARQLSADFYARLRAAAAAADASGAETLCRDVMR
                     ASQATWARRGEE"
     gene            6167..>6746
                     /locus_tag="PK829_05645"
     CDS             6167..>6746
                     /locus_tag="PK829_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40731.1"
                     /translation="MSNADSSDDRAKKPPAASSPGTGKKSKSKGFIESSDFKPGWLET
                     GPLPPDVLPSLPDPAKETSLADELAALQETLQRLYGSDTPDAATEEPAPAEDNAPAPA
                     AQPRPEAPVAREAGPLFTDPLMAAEPSIIVPPSPTEPSAPEAVAPEAGGPEAVAPEAG
                     VPEAVAPEAGVPEAGVPEAVAPEAGAPEAVVPE"
BASE COUNT         1127 a         2352 c         2328 g          939 t
ORIGIN      
        1 ccagcggctg cgtggcgctc tgcacaaacg cgttcaagct caggggagtg gcgacactga
       61 gaaagaggca ggtgaagagg ctgccgaaca ggatgagcca ggccagatgc tgccggctaa
      121 agcgatggcc ctcggtcata cgccacgaac tcggaaatcg gtgaaggccc cgctagcgtc
      181 tttccacacg ctttcgacgc gctcgacacg cgccgatggc gaaccgtggt gcagaaaggt
      241 cagcagttgg cgcagagcct cggccggccc ctcgacgacg acccgcactg tgccgtcgga
      301 ttggttggca acccaaccaa ccagaccaag acggcgggct tcctgccagg tatattgtcg
      361 aaagtagacc ccctgcacga gaccatagac ggtggcctcc aggcaagatg ttggtggcga
      421 tgacataagg ggaattatag cgcggtcggc gggttcggct atcgttggtt gggtagccgg
      481 ggggcagtgg tcggccggta gccggccgtc taccccgcca gcccgcgtag gatgcggccg
      541 ggcaggcgtg gcccggcata gacgaggctg ctgtagagct gcaccacgga cgcgcccgcg
      601 tcgagcttct cgcgcgcgtc gctggccgag gcgatgccgc ccacgccgat gatcggcagc
      661 ttcccgtccg tccggcggta aatggcggcg atgagttccg tgctgcgcgc ccgcagcggc
      721 cggccgctca gcccgccggc ttcggcctgt cgccggtcgc gcagaccctc acgggccagc
      781 gtcgtgttcg tggcaatgac gccggccacg ccggcggcgg tcagcgcggt cagcacctcg
      841 tccagctcat cccaacccag gtcgggggcg atcttgaccc acagcggctt gagcgcgccg
      901 ccggccgacg tcgccaggat gcgactctcg gccatgagcg ccgccagcaa cccctccaga
      961 tagcgccctc cctgcaacgc gcgcaggccg ggtgtgttgg gcgaactgat gttgaccgcc
     1021 agatagtcgg catggttata gacggcgcgc atgacggcca cgtagtcggc cgcggcctcc
     1081 tccaacggcg tctccttctg cttgcccagg ctgacgccca gcacatagcc gcgcggccgg
     1141 gccgagaggg cttgcaggcg cggcccggcc gcagccacgc cgccgttggg aaagcccatg
     1201 cggttgatga gcgcctcgtc ggccggcagg cggaagatgc gcggccgggg gttgccagcc
     1261 tgcggccgcg gcgtcagcgt gccgacctcg atgtggccaa agcccagcgc cgccagcccg
     1321 acaaccacgc gggcatcttt gtcgaagccg gcagccatgc ccagcgggtt ggggaagcgc
     1381 agcgggccga tcagcaccgg cagcgccggc agccggccag ccatgcgccg caacgcaagg
     1441 cggccagccg gtgtggcctg cgccagagcc agagcggcca gcgtgcgctc gtgggccgtc
     1501 tccgggtcga agcgtttaaa gacgggaaag agcagttggg tatatagatc aggcatggcc
     1561 ggcctgctgg cggtcgcggc gtagaagccc gacgatgagc agcagcagcg ccgccaccag
     1621 tagcgccgcc gcaccacgcc gttgccgtgc ccggccgcgt gcctgccggg cgacggccgc
     1681 tcgctcccgg tcggcctgcg acgtggccaa cagcacgttc cagtcggaga tgtcgcggcg
     1741 gtcgggtaga gcgtaggccg cagcaaccag ctggcgatag cggcgctctt catccatcca
     1801 gcgcgcgtcg tcccaaccca actccggttg agcgatggcg cggatgtggg gcatcaggtc
     1861 gccgccgccg cgcggcaata gcagccccag ccgcacccgc cgcaagagca gatcgtccag
     1921 gtgggccacg ccctcggccc gcgccgccca acgcaactcc gcccaaagcg ccgtcgtgcc
     1981 cggaatggcc tccagatcgc ccggcccggc cgcggcaacg aggccggtcg ccgcgcgacc
     2041 atagcggcct agcagccggc agcgcacttc gtccgccagg cgctcgccgc cgggtaacga
     2101 gtccggcgcg gcctcatcgc cgctgaccgt ggccccagtg gccatccaca acagtggcgc
     2161 gtcgtcgtgc agccgggcca gccaggctgc gccatcgtcc acccgggcgc agacctgctc
     2221 tagcgcctgg cggccgacct ggcgaaaggt cgtcagtttg ccgccggtga cagtcagcaa
     2281 ccccgcctct tgccagacga cgtgatcgcg cgattcctca gacggatcga ccttgccgct
     2341 gccgatgacc ggccggatgc cggcaaaggt ggcgctgatg tcgtcgagcg tcagccccag
     2401 cgccgggaac tgcgccgtga cggcggccat gaggtaggcc acctcttcgc cggtgatgct
     2461 ggggtcagca tccagcggcg gcgggcaatc aacgtcggtt gtgcccagca gagtgacgcc
     2521 ctcccaggga aaggcgaaga ccggccgccc atcgaccggg tgcatgaagc tgatcgcctg
     2581 ggccacggga aagcgccagg cgggaaagac gagatggctg ccgcgcaggg gccgcaggcg
     2641 cgcctccccg cccacctgcc cacgcaggaa gtcggcccac gcgccggtgg cattgatgac
     2701 ggcgcgagcg cggacatcga ctggcaaccg gccggcttcg tcgccggacg tgtccaccag
     2761 ccggacgccg gcgacttcgc ccgcgtcgtc gcgcaatagc tcctcggcgc ggacgtagtt
     2821 gagcgccagc acctggcgct cagcgtcatc ggccgcgtca acggcctcgc gaatgagccg
     2881 caacaccagc cgggcatcat cggtctgggc gtcgttatac tggaagccgc cgcgcaggcc
     2941 ggttggggag atgtaggggg ccaacagctg gaagtcggcc gggctgtagt agcggtggct
     3001 ccattgcagg gccatcaggt catagacggc cagccccgcg ccatagacga gttgccccgc
     3061 gccgtcgccc tcgtactggg ccagcagaaa gccaagcgga tgcaccagcc ccgccccttc
     3121 ggccaataac tgctcccgcc cctgcaccga ggcccgcgtt agaccgacct ggccggtgcg
     3181 caaatagcgc agaccgccgt ggaccagctt cgacgagcgg ctcgacgtgc cccaggcgaa
     3241 gtcgcgctgc tccaccagga gcgcccgcag gcccagccgc gccgcctcgt gcagaatgcc
     3301 cgcgccggtg atgccgccgc cgacgacgac gatgtcccaa tcggcgctca gctgctgcca
     3361 ggcccgcgcc cgccaggccc tattccagtg tgtcatggtc tacctccgcg acggccctcc
     3421 ggctggccac ccgccccgtc cgcggccaac aggacgccag ggttcatgtg cccggcgggg
     3481 tcgaagcagc gtgccgccgc ggccagcgcg cccaggccca gcgcgccctt ctcagcggcc
     3541 aggtagggcg cgtgatcgcg gccgacgccg tgctggtggc tgatcgtccc gccgccggcg
     3601 acgatggccc ggctggccgc gcccttcagc gcccgccagc ggtcgagcgt ctcgtccggg
     3661 ccggccgcca gccggaagag gtaggtcgta tagatgctgg ccccttgcgg gtagacgtgc
     3721 gacaggtggg tgaaaatgtg gacgcgctcg ccccggtcgt ccagcgcgcg gcccagagcg
     3781 tcctcgatct gtcccagcag acgcggcacg ttgtcccacg tcgtcgccgt ctccagcgta
     3841 tcgacggcgt agccccgctc ccagagagtg ttgcgcaggt aaggtgtgcg gaagcggccc
     3901 tcgatccact gcgcgccaaa gcgccgcccg gcggagacgc cgccctgctg gcggcagatg
     3961 tccagcacct ggtcacgcgc gtcgcggatg gtcgctgccc ggccggtgaa accgcacagc
     4021 agcatgcact tctcgccgcc gacgccgcgg acgctcagca gcgtctccag caggccgatc
     4081 agccgctcgt gcccggccag ggccagcgtc gtctgcgtct cgcggggcgt gctgaggcgc
     4141 agcagcgaca gcggccagcg cccctggacg atctgccgcg tggccgtggt cgcgccggcg
     4201 aagtcgggga aaaagacggc gtggaactcc tcatgctccg gcagaggcgt cacgcgcacg
     4261 gtcgcttcgg tcaggatgcc cagccgcccc tcggagccga gcaccaactg gcgcaaatcg
     4321 gggccggcgg ccgaggcggg gaacgacggc agccgcagca cccccgcggg cgacagcagc
     4381 gtgccgccgg cgaagaggct ctcgatacgg ccatagccga gcgactgctg gccgctggag
     4441 cgcgtggcca cccagccgcc gagcgtggag tactcaaacg actgcgggaa gtggcccagg
     4501 gtgaagccgc gagcgcgcaa ttgcgcttcc agctctggcc cggccacgcc cgcgccaaac
     4561 gtggccagat ggctgacgct gtcgaagcgc aacagtgcgt tcatccgccg tagactgacc
     4621 gtcagcgccg gcgcgtcgcc cggctcgggg ttgatgtggc cgacgacgct ggtgccgccg
     4681 ccgtagggga tgagccgcgc cccgaccgca gcggcgaagg acagcagccc ggccacgtcg
     4741 ctctcggtca gcgggtaggc gacgccgtcg gggaaggcgg ccacacgccc gctgcgcagg
     4801 gccacccagt cgggcaggct ttggccgcgg gcgtggcgca gccggtcggc cgcttccgcg
     4861 ctgatgagcg gatgggacgg caggcggcca ggcggcacag tggcggccac ctccgcgaag
     4921 gacgtgtcgc gcggcggcgt cccggggcca agccgctcct ccaggaaggc cggcgctgtg
     4981 gccggcagca ggtaggggat ggtatcgtcg ccccaaccat tccaacggcg catgtcactc
     5041 ctccccgcgc cgcgcccagg tggcctggct ggcgcgcatc acgtcgcgac agagcgtctc
     5101 ggccccgctg gcgtcggccg cagccgcggc ggcgcgcaac cgggcataaa agtccgccga
     5161 gagctggcgc gcttcgacag ttgcaaagta gcgccgggca atgtcctcat agaagccgcg
     5221 aaagccgttc aggatcagcg tatagatggg gttgcccgaa cgcacagtca acagatgatg
     5281 gagctgccag tcgaagcggg cgaagtcggc cggcgtctcc agcacggccg cgccgtcgag
     5341 aaaggtcgtc agcagggccg cgtcgcgggc gacggccgcg cgagtgtagg ccggagccag
     5401 gtgcaggcgc acctccagga gttgggcaat gaagtcgggc ggcaggtagt cgggatgctc
     5461 aaccagatgg ccgagcacgt tcagcccacc ctcctcccag tagttgttga ccagggtcag
     5521 cttgccgtgg gccaccgtca gccagccgtc gcgggccagc cgttgcaacg cttcgcgcaa
     5581 cgtcggccgg gtgacgccta attgcgccgc cagcgtgcgc tcgccgggca gcgtcgtccc
     5641 cggcggaaac gtgccatcga ggatggccgc caacagaacg ctttcggcat gggcgcgggg
     5701 acgaacgggg gcaagccact cagtcatact gtcttccgtg gcggtgcggg gtggtcacac
     5761 ggcttccacg caccggtcag gccaacggcc ataacctctg gtcagaccac ttaccagttg
     5821 ccgacatcat acccggtagt ggcgatagct gtcaagtcca ttgcagagct ttgccaaagt
     5881 cgtctggcga ctattgacct gacgggtgga cataccattc gtggctgccc acctatcagg
     5941 tcggtgcgcc tggggtgact ttggcaaagc cctgaagtcc attgagccaa acgggcgaat
     6001 tgtgggcaag atggctatag agaaggatgg tttcttcacg tataattcac cgtggattta
     6061 ctaccttttt cagcggcaat ggcgcgagtg atgttatctt cataggatgg gcgttggccg
     6121 acccgtaatc gtgtctgtct agttggccga gcagtcgaga tgtattatga gcaacgcaga
     6181 ttcgtccgac gacagggcaa agaaaccacc cgcagcctct tctccgggaa cgggcaagaa
     6241 gagtaagtct aagggcttca ttgagtcgtc cgacttcaag cccggctggc tggagaccgg
     6301 cccgctgcca cccgacgtat tgccttcgct acccgatccc gctaaggaaa cgtctttggc
     6361 cgacgaactg gccgcgctgc aagagacgtt gcagcgcctc tatggcagcg acacacccga
     6421 cgccgccacg gaggagccgg cccccgcaga ggataacgcg cccgcccctg ccgcccagcc
     6481 gcggccggaa gcgcccgtcg cgcgcgaggc tggaccgctt ttcaccgatc cgttaatggc
     6541 agcagagccg tccatcatcg ttcctccatc gcccacggag ccatctgcgc ctgaagcagt
     6601 cgcgcctgaa gcaggcgggc ccgaagcagt cgcgcctgaa gcaggcgtgc ccgaagcagt
     6661 cgcgcccgaa gcaggcgtgc ctgaagcagg cgtgcctgaa gcagtcgcgc ctgaagcagg
     6721 tgcgcccgaa gcagtcgtgc ccgaag
//
LOCUS       DAOSMQ010000125         6736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11401_length_6736_cov_3.979793, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000125 DAOSMQ010000000
VERSION     DAOSMQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11401_length_6736_cov_3.979793"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK829_05650"
     CDS             complement(<1..351)
                     /locus_tag="PK829_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40732.1"
                     /translation="MPALPVVEWWSRRSRRRQPRHTAMPFADPPPDSKLPPPLFDLCN
                     RLTTSFSLDELKDLAFTIGIDPESVADDTRPEFGLGLARAAWCRERLEALLAEAARQV
                     PGVDWLLPALPARPA"
     gene            complement(620..1933)
                     /locus_tag="PK829_05655"
     CDS             complement(620..1933)
                     /locus_tag="PK829_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU40733.1"
                     /translation="MAHANLNPDRDYDRLQRALDRQVTGAPASPAFMAILRLLYAPEE
                     AALAARVPILPTPLDKLAGQLGRDPEELDGQLTRLAERGLILDLSANGRRYYALPPVV
                     IGFFEFTFMRARPDMPMAELARLFETYMTEDDGRFARAVFDQSTQLGRSLPRESALPP
                     SPAVEVLDYEKASAIIRDARAVAVSLCACRHKAAHLGHACDRPQRTCLTFGGSSGMLV
                     PNGMAESIDRAEALDILAECQAAGLAQTADNVKHGISYMCNCCGDCCGMMRAIREFDL
                     PHAIVTSNWIMSIDYAQCNGCGRCVAACPVDAIHLDERVEERPGGRRHRTRWAVVDEA
                     LCLGCGTCSGACQFGGLALEPRPQRVYTPDNLVERVVAMAVERGKLADVVFGEPEKLS
                     RRAVANVVRAIEKLPPTKALLGSAAVKSAFLGAVARGTARFVKTP"
     gene            2282..>5468
                     /locus_tag="PK829_05660"
     CDS             2282..>5468
                     /locus_tag="PK829_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOU40734.1"
                     /translation="MSVPTVPTSARTLVATSVATRFRVFRLLAAAVFAAALLLSLRPP
                     VEAANYRTHIARVPDTPSNTQTVRIWINSDTVSGETAGVETLINGVYTKHLGTFQNGG
                     IGGYPGANWRVDLPAQPAGTFVQYQLFTRNQSGGDYGFDGFNYSYTVNGGDVQWNDLY
                     HNTFDSWARFPSGAVAAGTEVTLRLHTAPGDADSVALRVYTYNPATDNTSGPVDYAMS
                     YQQTNWYPTPVVGQWSYTLATPASPAILYYHFVITDAADVDYYGDDHSGPHDNLNQGG
                     TGAASDNQGAEGFQLTVYANGFQTPDWLQDAVVYQIFPDRFRNGNTNNDYCRAGGTTG
                     CPLFYGDQDALLREPWNTLIGDPRQPGPYQNQYGTQFYGGDLQGLIDKLDYLKAAGFD
                     TIYTTPIFDGSSNHRYDTDNYMAIDPALGTQADFDALIAALNARGMHLIVDGVFNHTS
                     SDSVYFDRYHRYSGNNGACESLGSTYRDWYNFYNNDAPCGTGDYEGWFGYDSLAVLQD
                     GSAEVRDTIYRDANSVVDTWYDRGVAGWRFDVADEISHDWWRDFRPYAKADAPNGPLV
                     GEVWPDASAFLLGDQLDSVMNYRFRKNVLGFVRQAGFADNDNNGNNEIIALSPSQFDR
                     ALRSVREDYPPQATAAMLNLLDSHDTNRLRYLVDIVGESDAQTLQRQKLAALFQYTYM
                     GAPMVYYGDEVALDSPSLANGVNGPEDDPYNRAPYPWADEAGDANAYGPADADMLAYY
                     TRLGELRRQYPFLSDGDFYTLVTGDTTTWTSDDDTTYVFARTGSSETVFVALNPGNNA
                     NTVIVSSGLIDYGSVWEDMLTGDIYNAPYGGFSVTLPPRTGVFLAKAVTSLTVAKTVN
                     GDATGLNDWQFTVNGNGGYTTPAAGGEFTITGIITGTTTVAETTVGGWTPAVSCDNGA
                     TGTDAATFNLDTGEDVVCTFTNTQDAPPSGDLTITKTVVGTPPGADWAFTGDLGPFNL
                     PASGGGATYSDQAVGDYTINETYVPGWATTTSCTPGSETGGRSVSLTLDDGEAAECVF
                     TNTLCQPGFYDDMSACVPTDPGYYTDASGAVA"
     assembly_gap    5469..5568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5569..>6736
                     /locus_tag="PK829_05665"
     CDS             <5569..>6736
                     /locus_tag="PK829_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40735.1"
                     /translation="KTTDPAGGTGFPFTLDPGAATFAGKWGVNGVGQGDFNGPFGIAV
                     DGDGNVYVADTFAYRVQKFDADGNFLLMWGRDVQVGGVTGFEICLASETCKAGTNGSG
                     NGEFNLPHGVAIDAAGNVYVSDSSNDRIQKFDTDGNYVGQWGGLGDEGGQFDNPTGVA
                     VDGAGNVYVADSFNNRIQKFGPAGNFLFTWGYDVLDDGTSHADFEICTASDDCWAGIG
                     GNGDGQLDDPFGVAVYGGNVYVADTFNHRVQVFATDGSYQDKWGSEGSGDGQFKYPYG
                     LTTDAAGNVYVADTNFFTGNTNDRLQKFNAGGGYLGQWGSEGINDGQFKSPTGVAASG
                     ASIYVTEYEGHRVQSFSQTGATLDDGQSHAFSDLEPDTYLVSELAPTGWALSDID"
BASE COUNT         1158 a         2348 c         2146 g          984 t
ORIGIN      
        1 cgccggccgg gcgggcaggg cgggcagcag ccagtcgacg ccgggcactt gccgggcggc
       61 ttcggccagc agggcttcca ggcgttcgcg gcaccaggcg gcgcgggcca gccccagacc
      121 aaactccggt cgcgtgtcgt cggccacgct ctccgggtcg atgccgatgg tgaaggccag
      181 gtctttcagt tcatccaggc tgaaggaagt tgtcaggcga ttgcagaggt caaagagggg
      241 cggcggcaac ttcgagtccg gcggcgggtc ggcaaacggc atggctgtat ggcgcggttg
      301 ccgccgccgc gaacgtcgcg accaccactc cacgactggt aacgctggca tgacgcctat
      361 cgcctcccgc tagtaagcat acacggccct acccggcgcg cgaccgccgg caatcgctgt
      421 cgatggtatt gtcgccgggc gactggccgg cggcaaaggg ctgggttgat ggcactacga
      481 ccgaagtaca cccatcttag caaaagggcc ggggcgttgt ccagagagac gggagttaaa
      541 ccgcctgatc cgggagtctg cgcgcgcggt cagggcgtct tgacgaagcg ggcgctgcca
      601 cgggccaccg tgggcgcggt cagggcgtct tgacaaagcg ggctgtgcca cgggccaccg
      661 ctcccagaaa agccgacttc accgccgcgc tgcccagcag cgccttggtt ggcggcagct
      721 tctcgatggc gcggacgacg ttggccacgg cgcggcggct cagcttctct ggctcgccaa
      781 agacgacgtc ggccagcttg ccgcgctcga cggccatggc cacgacgcgc tcgacgaggt
      841 tgtccggcgt gtagacgcgc tgcggccgcg gctccagcgc caggccgccg aattggcacg
      901 cgccgctgca cgtgccgcag cccaggcaga gggcttcatc gacgacggcc cagcgcgtgc
      961 ggtggcggcg gccgccgggg cgctcctcga cgcgctcatc caggtggata gcgtcgacgg
     1021 ggcaggcggc gacacaccgg ccacagccgt tgcattgcgc gtagtcaatc gacattatcc
     1081 agttggaggt gacgatagcg tggggcaggt cgaactcgcg gatggcgcgc atcatgccgc
     1141 agcaatcgcc gcagcaattg cacatgtagc tgatgccgtg cttgacgttg tcggccgtct
     1201 gggccagccc ggcggcctgg cactcggcca gaatatccag cgcctcggcc cggtcgatgc
     1261 tctcggccat gccgttgggg acgagcatgc cgctgctgcc gccaaaggtc aggcaggtgc
     1321 gttgcggccg gtcgcaggcg tggcccaggt gggcggcttt gtggcgacag gcgcacaggg
     1381 acacggccac ggcgcgggcg tcgcggatga tggcgctggc cttctcatag tcaagcacct
     1441 cgaccgccgg cgacggcggc agcgccgact cgcgcggcag ggagcggccg agttgcgtgc
     1501 tctggtcgaa gacggcgcgg gcgaagcggc cgtcgtcctc ggtcatgtag gtctcgaaca
     1561 gccgcgccag ttcggccatg ggcatgtcgg gccgggcgcg catgaaggtg aactcgaaga
     1621 agccgatgac gaccggcggc agggcgtagt agcggcggcc gttggcgctc aggtcgagga
     1681 tgaggccgcg ctcggccagg cgggtcagtt gcccgtccag ttcctccggg tcgcggccga
     1741 gttggccggc caacttgtcc agcggcgtgg gcaggatggg cacgcgggcg gccagggcgg
     1801 cctcctccgg cgcgtagagc aggcgcagga tggccatgaa ggcgggcgag gcgggcgcgc
     1861 cggtcacctg ccggtcgagg gcgcgctgca aacggtcgta atcgcggtcg gggttgaggt
     1921 tggcgtgggc cacgggcttg ctcccgcgtc gccggcccgg cgaaagccgg cgcggcgacg
     1981 ctgcccacag cttaggacag cccgcgctgg ttgtccagtg acaaagatca ccttttggct
     2041 ctgttgccag gctcgcggcg cgctgggcgg cgcggcgcgg cgcagcttct ggggccgcgc
     2101 acctgtcagg tctcgagcaa ctgacgactt tggaaaagcc ccgatcatgg caaaatagta
     2161 cttgacggcc gtgggcacat atgtataatg tcccgctacg ggagaagggc agttctgttg
     2221 gagctgccct ttttcaattg acaactggtt cccatcctgc cggcacgtcc tgaggttacc
     2281 catgagcgtt cccacggttc ccacgtctgc gcgcaccctg gtggccacgt ctgtggccac
     2341 tcgcttccgt gtctttcgcc tgctggccgc ggccgtcttc gccgcggccc tgttgttgtc
     2401 gctgcggccg ccggtcgagg cggcgaacta ccgcacccac atcgcccgcg tgcccgacac
     2461 gcccagcaac acccagaccg tgcgcatctg gatcaacagc gacaccgtct caggagaaac
     2521 ggccggcgtc gagacgttaa tcaacggcgt ctacaccaag catctgggca cgttccagaa
     2581 cggcggcatt ggcggctatc ccggcgccaa ctggcgcgtc gatctgcccg cccagcccgc
     2641 cggcacgttc gtccagtacc aactcttcac ccgcaaccag agcggcggcg actacggctt
     2701 cgacggtttc aactatagtt acaccgtcaa cggcggcgac gtccagtgga acgacctcta
     2761 tcacaacacc ttcgatagct gggcccgctt cccctccggc gcggtggcgg ccgggacgga
     2821 agtgacgctg cgcctacaca ccgcgcccgg cgacgcggac agtgtcgccc tgcgcgtcta
     2881 cacctataat ccggccacgg acaacacgtc cggcccggtc gactacgcca tgagctacca
     2941 gcagactaac tggtacccca ctcccgtcgt cggccaatgg agctacaccc tggccacgcc
     3001 ggcctcgccg gccatcctct actaccactt cgtcatcacc gacgcggcgg acgtggacta
     3061 ctacggcgac gaccacagcg gcccccacga caacctgaac cagggcggca cgggcgcggc
     3121 cagcgacaac cagggggccg agggcttcca actcaccgtc tacgccaatg gcttccagac
     3181 gcccgactgg ttgcaggacg ccgtcgtcta ccagatcttc cccgaccgct tccgcaacgg
     3241 caacaccaac aacgactact gccgcgccgg cggcacgacc ggctgcccgc tattctatgg
     3301 cgaccaggac gcgctgctgc gcgagccgtg gaacacgctc atcggcgacc cgcgccagcc
     3361 cggcccctac cagaaccagt acggcacgca gttctacggc ggcgacctgc aagggctgat
     3421 cgacaagctc gactacctga aggcggccgg cttcgacacc atctacacca cgcccatctt
     3481 cgacggcagc tccaatcatc gctacgacac cgacaactac atggccatcg acccggcgct
     3541 gggcacgcag gccgacttcg acgccctcat cgccgccctg aacgcccgcg gcatgcacct
     3601 catcgtcgat ggcgtcttca accacacctc gtccgacagc gtctacttcg accgctacca
     3661 tcgctacagc ggcaacaacg gcgcgtgcga gtcgctcggt tccacctacc gcgactggta
     3721 caacttctac aacaacgatg ccccctgcgg cacaggcgac tacgagggct ggttcggcta
     3781 cgacagcctg gccgtgctgc aagacggcag cgccgaagtg cgcgacacca tctaccgcga
     3841 cgccaacagc gtcgtcgata cctggtatga ccgcggcgtg gccggctggc gcttcgacgt
     3901 ggccgacgag atcagccacg actggtggcg cgatttccgt ccctacgcca aggccgacgc
     3961 ccccaacggc ccgctggtgg gcgaggtctg gcccgacgcc tccgccttcc tgctgggcga
     4021 ccagctcgac tcggtcatga actaccgctt ccgcaagaac gtcctgggct tcgtgcgcca
     4081 ggcgggcttt gccgacaacg acaacaacgg caataacgag atcatcgccc tctcgcccag
     4141 ccagttcgac cgggcgctga ggtcggtgcg cgaggactac ccgccccagg ccacggcggc
     4201 catgctcaac ctgctcgata gccacgacac caaccgcctg cgctacctgg tggacatcgt
     4261 cggcgagagc gacgcccaga cgctgcaacg gcagaagctg gccgccctgt tccagtacac
     4321 ctacatgggc gcgccgatgg tctactacgg cgacgaagtg gccctcgaca gcccctcgct
     4381 ggccaacggc gtcaacgggc cggaggacga cccctacaac cgcgccccct acccctgggc
     4441 cgacgaggcc ggcgacgcca acgcctatgg cccggccgac gccgacatgc tggcctacta
     4501 cacgcggctg ggcgagttgc ggcggcagta ccccttcctc agtgacggcg acttctatac
     4561 gctggtgacc ggcgacacga ccacgtggac gtcggacgac gacaccacct acgtcttcgc
     4621 ccgcacgggc agcagcgaga cggtcttcgt ggctctgaac cccggcaata acgccaacac
     4681 agtcatcgtg tccagcggcc tcattgacta tgggtcggtc tgggaagaca tgctgaccgg
     4741 cgacatctac aacgctccct atggcggctt ttccgtgacc ctgccgccgc ggacgggcgt
     4801 cttcctggcc aaggccgtca cgtcgctcac cgtcgccaag acggtcaatg gcgacgccac
     4861 cggcctgaac gactggcagt tcaccgtcaa cggcaacggc ggctacacca cgcccgccgc
     4921 cggcggcgag ttcaccatca ccggcatcat caccggcacg acgaccgtgg ccgagacgac
     4981 ggttggcggc tggacgccgg ccgtcagctg cgacaacggc gcgacgggca cggacgcggc
     5041 gaccttcaac ctcgacaccg gcgaggacgt cgtctgcacc ttcaccaaca cccaggacgc
     5101 gccgcccagc ggcgacctga ccatcaccaa gaccgtcgtc ggcacgccgc cgggcgcgga
     5161 ctgggccttc accggcgacc tcggcccgtt caacctgccc gcctcgggcg gcggcgcgac
     5221 ctatagcgac caggccgtgg gcgactacac catcaacgag acctacgtcc ccggctgggc
     5281 gacgacgacc agttgcaccc ccggcagcga gacaggcggc cgcagcgtgt cgctgacgct
     5341 ggacgacggc gaggcggccg agtgtgtctt caccaacacc ctctgccagc ccggcttcta
     5401 cgacgacatg agcgcctgtg tgccgaccga ccccggctac tacaccgacg cgtccggcgc
     5461 ggtggcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca agaccaccga
     5581 cccggccggc ggcacgggct tccccttcac cctcgacccc ggcgcggcga ccttcgccgg
     5641 caagtggggc gtcaatggcg tcggccaggg cgatttcaac ggccccttcg gcatcgccgt
     5701 ggacggcgac ggcaacgtct acgtcgccga caccttcgcc tatcgcgtcc agaagttcga
     5761 cgctgacggc aacttcctcc tgatgtgggg ccgcgacgtg caggttggcg gcgtcacagg
     5821 cttcgagatc tgcctggcca gcgagacgtg caaggccggc acgaatggca gcggcaacgg
     5881 cgagttcaac ctgccgcacg gcgtcgccat tgacgcggcc ggcaacgtct acgtcagcga
     5941 ctcgtccaac gaccgcatcc agaagttcga caccgacggc aactacgtgg gccagtgggg
     6001 cggcctcggc gacgagggcg ggcagttcga caacccaacc ggcgtggccg tggacggcgc
     6061 gggcaacgtc tacgtggccg actcgttcaa taaccgcatc cagaagttcg gcccggcggg
     6121 caacttcctg ttcacctggg gctatgacgt gctcgacgac ggcacgtccc atgcggactt
     6181 tgagatctgc acggcgtccg acgactgttg ggccggcatc ggcggcaacg gcgacggcca
     6241 actcgacgat ccgttcggcg tggccgtcta tggcggcaac gtctacgtgg ccgatacgtt
     6301 caatcaccgc gtccaggtgt tcgccaccga tggcagctac caggataagt ggggcagcga
     6361 aggcagcggc gacggccagt tcaaataccc gtatggcctg acgaccgacg cggccggtaa
     6421 cgtctacgtc gctgacacca acttcttcac cggcaacacc aacgaccgcc tccagaagtt
     6481 caacgccggc ggtggctatc tgggccagtg gggcagcgag ggcatcaacg acggccaatt
     6541 caaatcgccc accggcgtgg ccgcgtccgg cgcgagcatc tacgtcactg aatacgaggg
     6601 gcatcgtgtc cagagcttca gccagacggg cgcgacgctc gacgacggcc agagccacgc
     6661 cttcagtgac ctggaaccgg atacctatct ggtcagcgag ctggctccca ccggctgggc
     6721 gcttagcgac atcgac
//
LOCUS       DAOSMQ010000126         6728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11423_length_6728_cov_4.757231, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000126 DAOSMQ010000000
VERSION     DAOSMQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11423_length_6728_cov_4.757231"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PK829_05670"
     CDS             <1..495
                     /locus_tag="PK829_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU40736.1"
                     /translation="VAVGVAMDCKLRGLDNRIVVITGDGELDEGTVWEALQVAAAKKL
                     DNLLFIVDRNHFQANIRTEELIPLEPLAPKFEAFGGRVRRVDGHDFAALEAGLRELSD
                     DGGSGKPAVLIADTIRGRGLPSIQERADRWFCNFSHEEVAALLDELHGARQAEITSEA
                     LIVR"
     gene            512..1444
                     /locus_tag="PK829_05675"
     CDS             512..1444
                     /locus_tag="PK829_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU40737.1"
                     /translation="MKNYEQTLLELAQADERIIVMTAENRAAIRNLPNLLPGRFIDTG
                     ITEQTMVGAAAGLALRGRIPVLHALATFLTLRAFEFVRTDVGIGGLPVKLVGGVPGFL
                     SDGNGPTHQAIEDVALMRGIPGFHVFCPADEEDLVLALPQIIHSPNPTYIRHNALKTV
                     AGYEHEPFEIGKAEVRRTSESATRPDDVAILVYGMLLAQAWEAKARLEAEGLSVRLVN
                     VRMPKPIDVAAVLGAARESSLLVTLEDHFLTGGLFSIVSEVLARHGETADVLPIALEE
                     HWFKPALLPDVLRHEGFTGEQIAARILERLGVLA"
     gene            1560..2897
                     /locus_tag="PK829_05680"
     CDS             1560..2897
                     /locus_tag="PK829_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU40738.1"
                     /translation="MPNKVQLDQEYPVITESQRLYERATGLIPAYAQTLAKGPTQYVD
                     GVAPKYLQRAKGSHVWDVDGNEYLDYNMAIGPISLGYGYPAVDEAIRRQLEDGIAFSL
                     IHPLEVEVAELVREVIPNAESVRYAKAGAEVTSAAVRLARAFTGRDKVLCCGYHGWHD
                     WYIGITARNAGVPQAVKDLVYTFNYNDMASVHAALDADTACVILEPMTFDFPRGDFLH
                     ELKAACAANGSLLIFDEMWTGFRWALGGAQEYFGVTADLACYSKAIANGMPIAVLAGR
                     ADVMSLLDEQVFFFTTFGGEALSLAAAAATIHELRDKNVPADLAAKGQRLMDGYNDIA
                     TELGLRDVTHCKGHPARGLIFFDDKGGQGLLMKSLVQQELLRHGILWSGFHNMCYSHS
                     AADIDHTLAAYGRALAELKGALAEGDLASRLRGKPVQPVFRRTDSFNLKPAKK"
     gene            2976..3785
                     /locus_tag="PK829_05685"
     CDS             2976..3785
                     /locus_tag="PK829_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU40739.1"
                     /translation="MSNELFTLDNKTAVVTGALGLIGRQHCAALAAHGANVIVADLDA
                     DACAAFAATLPAPALGCALDVTDPATISALRDAVLARFGHLDVLVNNAAINDMFESPA
                     MAAEQSRFENYPLAMWRQSLEVNVTGVFLCAQILGAPMAAQGHGSIINVASTYGIVAP
                     DQSLYRTPDGRQPFYKSPAYPTTKGAVIAFTRFLAAYWGQAGVRVNTLSPGGVENNQQ
                     PYFIDNYAARTMLGRMAAPTDYQGALIFLASDASAYMTGANLVVDGGWTAW"
     gene            3871..4449
                     /locus_tag="PK829_05690"
     CDS             3871..4449
                     /locus_tag="PK829_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOU40740.1"
                     /translation="MTEETLAIASRSSASESGDPAVGGRPSAVAESARHIRFVLTDSD
                     GVLTDNGVYYSAAGEEMKRFSIRDGMGVERLRVFAGVEVGIITGERSGAVQRRAEKLG
                     IIELHLGIRDKSAVLDELLARRGLTAGQVAYIGDDTNDVAIMRRVGLAGCPADATPFA
                     RAVAHYVCPSRGGYGAFRDFAEFIIAAKAEGA"
     gene            4640..5548
                     /locus_tag="PK829_05695"
     CDS             4640..5548
                     /locus_tag="PK829_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOU40741.1"
                     /translation="MHNNAMVDIGRRRIGDGEPVYIIAEIGINHNGSLELARKLIDGA
                     IFAGADAVKFQKRTPELCVPRDQWHLERDTPWGRITYLEYRHKVEFTYEQYRTIDRYC
                     RARNLAWFASCWDEQAVDFMEQFDTPLYKVASATLTDHALLDKLRATGRPMMLSTGMS
                     EMWQIDAAVERVGRHDLMIAHATSSYPCPPEELNLRMIPTLKAAYPDVPIGYSGHEVG
                     LAPTWAAVTLGATFVERHITLDRAMWGSDQAASVEVMGFHNLVRNIRDIERSLGDGVK
                     HVYPSEIAAMKKLRRVNTAEPSHANS"
     gene            5535..6650
                     /locus_tag="PK829_05700"
     CDS             5535..6650
                     /locus_tag="PK829_05700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40742.1"
                     /translation="MQIRKLDTTRKDERKRFVDFAFDLYRDAPLWVPPLYGSTLKILD
                     ASKHPYYEHSIADFYVAEDNGRVVGRVATLENRNYNAFHKSKTAFFGYFETVEDIGVA
                     RALLQTAKEWAAAHGLDTILGTRGVAGIDGSVLVDGFEHRPAIGIPWNYPYYDAFIRD
                     SGFEKDTDYLSGYAPGDIAIPPRLYEIAAKVRERRGFWIKTFKSRGEIRAWIPRALEI
                     HSQAMSGLHTYYPPTEAETRELIGSLLAIVDPSLIKLVMKGEDIAGFIIAYPDVSAGI
                     QKARGRLFPFGWAYILRDRQRTEWCNVNGLGLLPQYRGLGANAMLYTELRDTIAAHGF
                     KHIDTVQANETNFNSTSDHETLGVTWYKRHRHYKLSL"
BASE COUNT         1233 a         2349 c         2063 g         1083 t
ORIGIN      
        1 gtcgccgtcg gcgtggcgat ggactgtaag ctgcgcgggc tggacaaccg catcgtcgtc
       61 atcaccggcg acggcgagct ggacgaaggc accgtctggg aggcgttgca agtggccgcg
      121 gccaaaaagc tcgacaacct gctcttcatc gtggatcgca accacttcca ggccaatatc
      181 cgcaccgagg aactgattcc gctggagccg ctggccccca agttcgaggc gttcggcggc
      241 cgcgtgcggc gcgtggacgg ccacgacttc gccgcgctgg aagccgggct gcgcgagttg
      301 agcgacgacg gcggctccgg caagccggcg gtgctcatcg ccgacaccat ccgcggccgg
      361 ggcctgccca gcatccagga gcgcgccgac cgctggttct gcaacttcag ccacgaagag
      421 gtcgcggcgc tgctcgacga actgcacggc gcgcgccagg ccgagatcac ctccgaagcg
      481 ctcatcgtcc gttagccacg ggaggacacc catgaagaac tacgaacaaa ccctgctgga
      541 actggcccag gccgacgagc gcatcatcgt catgacggcc gagaaccgcg ccgccatccg
      601 caacctgccc aacctgctgc cgggacgctt catcgacacc ggcatcaccg aacagacgat
      661 ggtcggcgcg gccgcgggcc tggccctgcg cgggcgcatc cctgtgctgc acgccctggc
      721 cacttttctg accttgcgcg cctttgagtt cgtgcgcacc gacgtgggca tcggcggcct
      781 gccggtcaag ctcgtcggcg gcgtgcctgg cttcctgtcc gacggcaacg ggccgacgca
      841 ccaggccatt gaagatgtcg ccctgatgcg cggcatcccc ggcttccacg ttttctgccc
      901 cgccgacgaa gaagacctgg tgctggccct gccccagatc atccacagtc ccaacccgac
      961 ctacattcgg cacaacgcgt tgaagaccgt cgccggctat gagcatgagc cgtttgagat
     1021 cgggaaagcg gaggtgcgac gcacctcaga aagcgcgacg cgcccggacg acgtggccat
     1081 cctcgtctac ggcatgctgc tggcccaggc gtgggaggcc aaggcgcggc tggaggcgga
     1141 ggggctgtcg gtgcgcctgg tcaacgtgcg catgcccaag cccatcgacg tggccgccgt
     1201 gctcggcgcg gcgcgggagt cgtcgctgct ggtcacgctg gaagaccact tcctgaccgg
     1261 cggcctcttc tccatcgtca gcgaagtgct ggcgcgacac ggcgagacgg ccgacgtgct
     1321 acccatcgcc ctggaggagc actggttcaa gcccgccctg ctgcccgacg tgctgcggca
     1381 cgagggtttt accggcgagc aaatcgccgc gcgcatcctt gagcgtcttg gcgtcttggc
     1441 gtgagactta aggaagagaa gaggctcacg caaagacgca gagacgcaaa gaagagaaga
     1501 atcttatttc tttgcgtctt ggcgtcttgg cgtgaggtct tctcttaagg agatatacca
     1561 tgcccaataa ggttcaactc gaccaggagt atccggtcat caccgagtcc cagcggctgt
     1621 acgaacgcgc cactggtctt atccccgcct acgcccagac gctggctaag gggccgacgc
     1681 agtacgttga tggcgtggct ccgaagtacc tacaacgggc caagggcagc cacgtctggg
     1741 acgtggacgg caacgaatac ctcgactaca acatggccat cggccccatc tccctcggct
     1801 acggctaccc ggccgtcgat gaggccatcc gccgccagtt agaggacggc atcgccttct
     1861 cgctcatcca cccgctggag gtggaagtgg ccgaactggt gcgcgaggtc atccccaacg
     1921 ctgagtccgt tcgctatgcg aaggcaggcg cggaagtcac cagcgccgcc gtccgcctcg
     1981 cccgcgcctt caccggccgc gacaaggttc tctgctgcgg ctaccacggc tggcacgact
     2041 ggtacatcgg catcaccgcc cgcaacgccg gcgtgcccca ggccgtcaaa gacctcgtct
     2101 ataccttcaa ctacaacgac atggcctccg tccacgccgc cctcgatgcc gacaccgcct
     2161 gcgtcatcct ggagccgatg accttcgact tccccagggg cgatttcctg cacgaactca
     2221 aggccgcctg cgccgccaac ggcagcctgc tcatcttcga cgaaatgtgg actggcttcc
     2281 gctgggcgct gggcggggca caggagtact tcggcgtcac cgccgacctg gcctgctact
     2341 ccaaggccat cgccaacggc atgcccatcg ccgtgctggc cggccgcgcc gacgtgatga
     2401 gcctgctcga cgagcaggtc ttcttcttca cgaccttcgg cggcgaggcg ctgtcgctgg
     2461 cggcggcggc ggcgaccatc cacgaactgc gcgacaagaa cgtgcccgcc gacctggccg
     2521 ccaaggggca gcgcctcatg gacggctaca acgacatcgc cacggaactg ggcctccgcg
     2581 acgtgaccca ctgcaagggc caccccgccc gcggcctgat cttcttcgac gacaagggcg
     2641 gccaggggct gctgatgaag tcgctggtgc agcaagagct gctgcgccac ggcatcctct
     2701 ggtcgggctt ccacaacatg tgctacagcc acagcgccgc cgacatcgac cacaccctgg
     2761 cggcctacgg gcgggcgctg gccgagctaa aaggcgcgct ggcagagggc gacctggcct
     2821 cgcgcctgcg cggcaagccc gtccagcccg tcttccgccg cacggatagc tttaatttga
     2881 agccagctaa gaagtaggtt ccaggggcca ggttccaggg aaggcgcgct cttctctgga
     2941 acctggaacc tggccccttc ccctcggtat gcctgatgtc aaacgaactc ttcaccctcg
     3001 acaacaagac ggccgtcgtc accggcgcgc tggggctgat cggccgccag cactgcgcgg
     3061 cgctggccgc ccacggggct aacgtcatcg tcgccgacct ggacgccgat gcgtgcgccg
     3121 ccttcgcggc gacgctgccg gcgcctgccc tcggctgcgc cctcgacgtg accgacccgg
     3181 ccacgatcag cgccctccgc gacgccgtcc tggcccgctt cggccatctg gacgtgctgg
     3241 tcaacaacgc cgccatcaac gacatgttcg agtcgccggc catggccgcg gagcagtcgc
     3301 gcttcgagaa ctacccgctg gccatgtggc ggcaatcgct ggaggtcaac gtcaccggcg
     3361 tcttcctctg cgcccagatt ctcggcgcgc cgatggccgc ccaggggcac ggcagcatca
     3421 tcaacgtcgc ctcgacctac ggcatcgtcg cccccgacca gtcgctctac cgcacgcccg
     3481 acggccgcca gcccttctat aagtcgcccg cctacccgac gaccaagggc gcggtcatcg
     3541 ccttcacgcg cttcctggcc gcctactggg gccaggccgg cgtgcgcgtc aacaccctct
     3601 cgcctggcgg cgtggagaac aaccagcagc cctacttcat cgacaactac gccgcgcgga
     3661 cgatgctcgg ccgcatggcc gcgccgaccg actaccaggg cgcgctcatc ttcctggcct
     3721 ccgatgcctc ggcctacatg accggggcga atctggtggt cgatgggggg tggacggcgt
     3781 ggtgagcaaa cgaccgccga ccaccgaccg ccgacggctg ccaaagcgat tggtattgcc
     3841 cgacaacaga ccacgaacca tacacaaacc atgacagaag aaacgttggc tatagccagt
     3901 cgatcgtccg ccagcgaatc tggcgatccg gcggtcggcg gtcggccgtc ggcggtcgca
     3961 gagagcgctc gccacattcg ctttgtgctg accgacagcg atggcgtgct gaccgacaac
     4021 ggcgtttact actcggccgc gggcgaggag atgaaacgct tctccatccg cgacggtatg
     4081 ggcgtggagc ggttgcgcgt ctttgccggg gtcgaggtcg gcatcatcac cggcgagcgc
     4141 tccggcgcgg tgcagcggcg ggccgagaag ttgggcatta ttgagctgca cctgggcatc
     4201 cgcgacaaga gcgctgtgct ggacgaactg ctggcgcggc gcggcctgac ggccgggcag
     4261 gtggcctaca tcggcgacga caccaacgac gtcgccatca tgcgtcgcgt cggcctggcc
     4321 ggttgcccgg ccgacgcgac gcccttcgcc cgcgccgtgg cccactacgt ctgccccagc
     4381 cgcggcgggt atggcgcgtt tcgcgacttc gccgaattca tcatcgcggc caaagcggag
     4441 ggagcgtaag ggaagaggct cacgcaaagg acgccaagga agcaaagagc gctaagaaga
     4501 acaagataac tttctcttct ttgcgtcctt agcctcctta gcgtcctttg cgtgagcgtc
     4561 ttggcgtgag cttcttaaaa cctgcctaat ctgccgaatc cgcggataat ccctctggtt
     4621 gctgaacggg aggacaaata tgcacaacaa tgcaatggtc gatatcggcc gccgccgcat
     4681 cggcgacggc gagccggtct atatcatcgc cgagatcggc atcaaccaca acggctcgct
     4741 agagttggcc cgcaaactga tcgacggggc catcttcgcc ggggccgacg cggtcaagtt
     4801 ccagaagcgc acgccggagc tatgcgtgcc ccgcgatcag tggcatctgg agcgcgacac
     4861 gccctggggg cgcatcacct acctggaata ccgccacaag gtcgaattca cctacgagca
     4921 atatcgcacc atcgaccgct actgccgggc gcgcaacctg gcctggttcg cctcttgctg
     4981 ggatgagcag gccgtggact tcatggagca gttcgacacg cccctctaca aagtcgcctc
     5041 ggccacgctg accgaccacg ccctgctgga caagctgcgc gccaccggcc ggccgatgat
     5101 gctgtccacg ggcatgtcgg agatgtggca gatcgacgcc gccgtggagc gcgtcggccg
     5161 ccacgacctg atgatcgccc acgccacctc gtcctacccc tgtccgccgg aggaactcaa
     5221 tctgcgcatg atccccacgc tgaaggccgc ctaccccgac gtgcccatcg gctactccgg
     5281 ccacgaggtc ggcctggccc cgacgtgggc ggccgtcacc ctgggggcca ctttcgtcga
     5341 acgtcacatc accctcgacc gagccatgtg gggcagcgac caggccgcct ccgtcgaggt
     5401 catgggcttc cacaatctcg tgcgcaacat ccgcgacatc gagcgctcac tgggcgacgg
     5461 cgtcaaacac gtttacccca gcgagatcgc cgcgatgaag aagctgcgcc gggtcaacac
     5521 cgccgaacca agccatgcaa attcgtaaac tcgacaccac gcgcaaggac gaacgcaagc
     5581 gcttcgtcga cttcgccttt gacctctacc gcgacgctcc tctctgggtt ccgccgctct
     5641 acggttcaac cctgaagata ctggatgcca gcaagcatcc ctactacgag cattccatcg
     5701 ccgacttcta cgtggccgag gacaacgggc gggtcgtcgg ccgtgtggcc acgctggaga
     5761 accgcaacta caacgccttc cacaagagca agacggcctt cttcggctac tttgagacgg
     5821 tcgaggacat cggcgtggcc cgcgccctct tgcagacggc caaggagtgg gccgccgccc
     5881 acgggctgga caccattctg ggcacgcgcg gcgtggccgg catcgacggc agcgtcctgg
     5941 tggacggctt cgagcaccgg ccggccatcg gcatcccctg gaactacccc tactacgacg
     6001 ccttcatccg cgactccggc ttcgagaagg acaccgacta cctctccggc tacgcccccg
     6061 gcgatatcgc cattccgccg cgcctgtatg agatcgccgc gaaggtcaga gagcggcgcg
     6121 gcttctggat caagaccttc aagtcgcggg gcgagattcg cgcctggatt ccgcgggcgc
     6181 tggagattca cagccaggcg atgagcggcc tccacaccta ttacccgccc accgaggcgg
     6241 agacacgcga actgatcggc tcgctgctgg ccatcgtcga tccgtcgctg atcaagctgg
     6301 tcatgaaggg cgaggacatc gccggcttca tcatcgccta tcccgacgtt tccgccggca
     6361 tccagaaggc gcgggggcgg ctgtttccct ttggctgggc ctacatcctg cgcgaccggc
     6421 agcggacgga gtggtgcaac gtcaacgggc tggggctgct gccgcaatac cgcggcctgg
     6481 gggccaacgc catgctctac accgagctac gcgacaccat cgccgcccac ggcttcaagc
     6541 acatcgatac cgtccaggcc aacgagacga acttcaacag cacgtcagat cacgaaacat
     6601 tgggcgtgac gtggtataag cgccaccggc actacaagct atcactgtag aagaatccgc
     6661 cgttgatagg gactaggtgt cggctgcaaa gacatctcta ttgaaaaaac tgctagcaca
     6721 ttactaac
//
LOCUS       DAOSMQ010000127         6705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11470_length_6705_cov_3.790827, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000127 DAOSMQ010000000
VERSION     DAOSMQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11470_length_6705_cov_3.790827"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PK829_05705"
     CDS             complement(<1..418)
                     /locus_tag="PK829_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOU40743.1"
                     /translation="MSDTEKMIRSTAHNVLRMEQNPLEVMFKPKSVAVVGATERAGSV
                     ARTILWNLISTSFGGTIFPVNPKYNSILGIKSYPSIAAIPDPVDLAVIVTPAKTVPGI
                     IEECVNAGVSAAIIISAGFKETGPEGAALEQQILKIA"
     gene            complement(513..1118)
                     /gene="pyrE"
                     /locus_tag="PK829_05710"
     CDS             complement(513..1118)
                     /gene="pyrE"
                     /locus_tag="PK829_05710"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOU40744.1"
                     /translation="MIDLSPTEALVLTLHDIGAVQFGRFRLHSGQESRIYIDLRVLVS
                     FPAALRQATAAYRGLLAGLEYDLLAATPLAGLPIGTALCLDLDKPLIYPRKTAKSYGT
                     GKDIEGRWSIGQTAVVIDDLITSGDSLLETIATVKAAGLKVHDTAVLIDREQGGREML
                     AEQGYVLHAVMSLRQLLEMLVVHDRLTYYQRREVLSSLNLA"
     gene            complement(1188..1958)
                     /locus_tag="PK829_05715"
     CDS             complement(1188..1958)
                     /locus_tag="PK829_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40745.1"
                     /translation="MNKKHLCAALAVLLVGGASIGCGNAPDENAALATTEATAVAVAP
                     TKPPPIIAANTAAPAPTSAEVAPPTPTAQPTMEITAAPTAAPTDVPPTPVPPTAVPPT
                     AVPATVAPPAPPAPTAIPPTAVPPTAAPPPSSPGSINGLSASNFAIQDRAQLTVNGDI
                     WFEFHVANSTNSDVPFDALGVMPKKDGVDRPQWYQHSWGGNNDVIPPDGLSWDDHINL
                     PEAGNYTLRLVICFDGPACQAQNGTWHTLSQEIPVTIR"
     gene            complement(1996..2880)
                     /locus_tag="PK829_05720"
     CDS             complement(1996..2880)
                     /locus_tag="PK829_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOU40746.1"
                     /translation="MSTFQLYAVEDAGPRPLPLPAGATQFIDLYRGLALGVYDAFRTF
                     DHNKFLYLNRHLARTERSMALLGWTYRLDRARLLRALHAVVTAAPWPEMRVRLDVLAA
                     PAEALGTTSRELIALQPFTPPPPELYETGVAVGFAAGLARQRPLAKTADFAAQRPATS
                     GLRGLESAAAGDQPYELLLLDSAGRILEGTGTNFWAVSDGTVYTAGDNVLEGITREIL
                     LELIPSLGLPLRLEAVAADDLGALTEAALSGSSRAFLPVVSIAGQPVGDGRPGPVSRR
                     ILAAYNAFVAANIKTANP"
     gene            3126..5567
                     /locus_tag="PK829_05725"
     CDS             3126..5567
                     /locus_tag="PK829_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40747.1"
                     /translation="MRHSLARIAVIVGLVAVGLWLAVRASMPAPESSARRAALSAFAP
                     GRPLAPASSAELAPQAGTAAPTTVVLANVPTGVDPADSLYARWQRGEVDLTETEGLLP
                     PAEVAALQEAALSLPPMPPQAAPPSTQAPVPGAGWASLDYNDGSGVPPDPELAVGPNH
                     VIAAVNSSFEIYNKSGALVGGPYGFASFLSEVPNCVDFDFDPNAIYDEAADRYVLGID
                     INGEYYCLAVSATNNPLGNWHVYAFPVGSAAEFFDYPHAGVGDQYIFAGANIFTSSFL
                     ESRVYAFDKGDMYAGNPADWASRALPTTEDTPIPLHLHGWAQGTWNTGANHYFLTDYN
                     YNGATYRVWRWSNPLSSAPTAVGVVDLEDYTGVVGGYPIDAPQSGSSARLQANDFRPQ
                     DFEYRNGYAWTAYTFSCNPGGGTVNCVRWAKIDPATATIADAGVLATAGQHRIFPNLA
                     VNRCDDMAVGYTKTSASSFPGVFATGRQASDPAGTLQAETQLQAGTITYTAFDGSPHR
                     WGDYTGMTIDPDGETFWYLGEYSKNTGTSNGRWATYVRSLAYETCGGGPGPTQLSVDL
                     SAKAAGAAGGVAFAANDIIHYDGATDTWSMLFDASDVGITKNVTAFYRQDNAATLDTF
                     YLVLAANQNVPGVGTVTPNDVIRFTPTSLGSSTAGSFALYFDGSDVGLTTTAEHIDAL
                     GMDGSRLLISTTGAAKVPRGGGGSLNAPDEDVIAFTPTSTGATTAGTWAAFFDGSTVP
                     GLAVEDVAGFWDNPANGNLVISVLNAFNLSGVTGDNDDLIGLTPSGGSYTPSIVWNGD
                     DAGFNVAIDAFEIVP"
     gene            5693..>6705
                     /locus_tag="PK829_05730"
     CDS             5693..>6705
                     /locus_tag="PK829_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40748.1"
                     /translation="MSSRGRPLSLLLPMALVALAALLALAARGVLARPLTEPARPAAA
                     PVLAVSDYTTATAGQPVVVPLAFSSGGHDIDTIFFVLSYDATLLTLDKTDANDDGVPD
                     SIVPTLPGGYRLIASIDIAGGEGRFQALIYPSPDSPPLPSGNLATLTFKVPWRPTPVP
                     TYLDFLTSPAPFFANTNDQVVPGSFVGGMVDINQIPTLTPTPSRTPTPTATPSRTPTA
                     TPTTTPTATTTGTATPTVTGTPPTPTPSPSPTVTATASATATASATASATPTAAPIYL
                     PALLYGASPTPTATASPTPTATPTASATLTSTPTASSTPTATITGTPPTPASTPTPSR
                     TPTA"
BASE COUNT         1090 a         2434 c         2134 g         1047 t
ORIGIN      
        1 gggcgatctt caggatctgc tgctccagcg ccgcgccctc cggccccgtc tccttgaagc
       61 cggccgagat aataatagcc gcgctgacgc cggcgttgac gcactcctcg atgatacccg
      121 gcaccgtctt ggccggcgtg acgatgacgg ccagatcgac ggggtcgggg atggcggcga
      181 tgctggggta cgacttgatg cccaggatgc tgttgtattt ggggttaacc gggaagatcg
      241 tcccgccgaa ggacgtggag atgaggttcc acaagatggt gcgggccacg ctgcccgcgc
      301 gctccgtcgc ccccacgacg gcgacgctct tcggcttgaa catcacctcc agtggattct
      361 gttccatgcg caacacattg tgggctgtgc tgcgaatcat cttctcggta tcagacatgg
      421 tgtgattccc cttatgcgcc tggacaggac ggcgcgcctg gcggccatcc gtctgtgtaa
      481 acccccaacc cgcggtgacg aggacgcgcg ggctaggcga gattgagtga cgacaggact
      541 tcgcgtcgct ggtaataggt caaccgatcg tggacgacca gcatctccaa caactggcgc
      601 agcgacatga cagcgtgcag cacatagccc tgctcggcca gcatttcgcg cccgccctgc
      661 tcgcggtcga tgagcacggc cgtgtcgtga accttcagcc cggcggcctt caccgtggca
      721 attgtctcca gcaggctgtc gccggaggtg atcaggtcgt cgatgactac cgccgtctgg
      781 ccaatcgacc agcgcccctc aatgtccttg cccgtgccat agctcttggc cgtcttgcgc
      841 gggtagatga gcggcttgtc caggtcaagg cacagcgccg tgccgatggg cagcccggcc
      901 agcggcgtgg ccgccagcag gtcatactcc agcccggcca gcagcccgcg ataggccgct
      961 gtcgcctgcc gcagggccgc cggaaacgac accagcacgc gcaaatcgat gtagatgcgc
     1021 gactcctggc cgctgtgcag tcgaaaacgg ccgaactgca ccgcgccgat gtcgtgcagg
     1081 gtcaggacga gcgcttccgt gggcgacaga tcgatcatcc gtctgattct agcaccacgc
     1141 ggccgaaaga gaaaagccaa cgcgataggg cggcagggct gttgctgtca gcggatggtc
     1201 acgggaatct cctgcgacag ggtatgccac gtgccgttct gcgcctggca ggccgggccg
     1261 tcgaagcaga tgaccagccg cagcgtgtag ttgcccgcct ccggcaggtt gatgtggtcg
     1321 tcccacgaca ggccgtccgg cgggatgacg tcgttgttgc cgccccaact gtgctggtac
     1381 cactgcggcc ggtcgacgcc gtctttcttg ggcatcaccc cgagcgcgtc gaagggtaca
     1441 tcgctgttgg tcgagttggc cacgtggaac tcgaaccaga tgtcgccgtt gacggtcagt
     1501 tgcgcccggt cttgaatggc gaagtttgag gcgctcaggc cgttgatgct gcccggcgac
     1561 gagggcggcg gcgcggccgt cggcggcacg gcggtggggg ggatggccgt cggcgcgggc
     1621 ggcgcgggcg gcgcgacagt ggccggcaca gccgtcggcg gcacggccgt cggcggcacg
     1681 ggcgtcggcg gcacgtcggt cggcgcggcc gtgggcgcgg ccgtaatctc catcgtcggc
     1741 tgagcggtgg gtgtcggcgg cgcgacctcg gccgacgtcg gcgcgggcgc ggccgtgttg
     1801 gcggcaatga tgggcggcgg cttggtgggc gcgacggcca cggccgtcgc ctcggtagtt
     1861 gccagggcgg cgttctcgtc cggcgcgtta ccgcagccga tggacgcgcc gcccaccagg
     1921 agcaccgcca gggcggcgca aagatgcttc ttattcacag tgtgtttgtc tccagtcgca
     1981 ggccggcggg ccggcctacg gattggcggt cttgatgttc gcggcgacga aggcattata
     2041 ggcggccagg atgcgacgcg agaccggccc ggggcggccg tcgcccaccg gctgcccggc
     2101 gatgctgacc accggcagga aggcccgcga tgagccgctc agcgcggcct ctgttagcgc
     2161 gccgaggtcg tccgcggcga ccgcctccag ccgcagcggc aggcccagtg acgggatcag
     2221 ctccagcagg atctcgcggg tgatgccctc cagcacattg tcgccggcgg tatacacagt
     2281 cccgtcgctc acggcccaga agttagtgcc cgtgccctcc agaatgcggc cggcgctgtc
     2341 gagcaggagc aactcatatg gctggtcgcc ggcggcggcc gactccagcc cccgcagccc
     2401 gctcgtggcc gggcgctgcg cggcgaagtc ggccgtcttg gccagcgggc gctggcgcgc
     2461 cagcccggca gcgaaaccga cggccacgcc agtctcatat agctctggcg gcggcggtgt
     2521 aaagggctgc aaggcgatca gctcgcggct ggttgtgccc aacgcctcgg ccggcgcggc
     2581 cagcacgtcc agccgcacgc gcatctccgg ccacggcgcg gccgtcacca cggcatggag
     2641 cgcccgcagc agccgcgccc ggtcgagccg gtaggtccag cccagcaagg ccatcgaccg
     2701 ctctgtgcgc gccaggtggc gattgagata gaggaacttg ttgtggtcga aagtacgaaa
     2761 ggcatcgtag acgcccagcg ccaggccacg gtaaaggtcg ataaactgcg tcgccccggc
     2821 cggcaggggc aacggacgcg ggccggcatc ctcgacggca tatagctgaa acgtagacat
     2881 aggtgaaatc tagcccaaag accagcggac gcaagtgccc aatgacctga ccagtgggtg
     2941 gccagtcgtt ttctggtggc agtgccatgc gtggccgccc accggtcagg tcagcgcgcc
     3001 gggggcgact ttggaaaaag ccctgcttcc ctgtcgcttc gggcagtccg gtgctatact
     3061 ggtacaagat tcattgtgaa tctgcaagtc gcgtggccgt ccatcaaacg aggagctaaa
     3121 cccacatgag acactcatta gccagaatcg ccgtcatcgt tggcctggtg gccgtgggcc
     3181 tgtggctggc cgtccgcgcc agtatgcccg cgccggagag cagcgcccgc cgcgcggcgc
     3241 tatcggcgtt tgcccccggc cggccgctgg ccccggccag cagcgccgag cttgcgcccc
     3301 aggcgggcac ggccgcgccg accactgtcg tgctggccaa tgtgcccacc ggcgtcgatc
     3361 cggccgacag cctctatgcc cgctggcagc gcggcgaggt agacctgacc gagacagagg
     3421 gcctcttgcc gccggccgag gtcgccgccc tgcaggaagc ggccctgtcg ttgcccccca
     3481 tgccgcccca ggccgcgccc ccctcgactc aggcgcccgt ccccggcgcc ggctgggcca
     3541 gcctcgatta caacgacggc agcggcgtgc cgcccgaccc agagctggcc gtcggcccga
     3601 atcacgtcat cgccgcggtc aactcatcct ttgaaatcta caacaagtcc ggcgcgctcg
     3661 tcggcggccc atatgggttt gccagcttcc tgagcgaagt gcccaactgc gttgacttcg
     3721 atttcgaccc caacgccatc tacgacgagg ccgccgatcg ctatgtcctg ggcatcgaca
     3781 tcaatggcga gtactactgc ctggccgtct cggcgaccaa caacccgctg ggcaactggc
     3841 acgtctacgc cttccccgtc ggcagcgcgg ccgagttctt cgactacccc catgccggcg
     3901 ttggcgacca gtacatcttc gccggggcca acatcttcac gagttccttc ctggaatcgc
     3961 gcgtctatgc cttcgacaag ggcgacatgt acgccggcaa tccggccgac tgggccagcc
     4021 gcgccctgcc cacgaccgag gacacgccca tccctctgca cctccacggc tgggcacagg
     4081 gcacctggaa caccggcgcg aaccactatt tccttaccga ctacaactac aatggcgcta
     4141 cctatcgcgt ctggcgttgg tcgaacccgc tgagcagcgc gcccacggcc gtcggcgtgg
     4201 tcgatctgga ggactacacc ggcgtcgtcg gcggctaccc catcgacgcc ccgcagagcg
     4261 gcagcagcgc ccggctccag gccaatgact tccggccgca ggacttcgag tatcgcaacg
     4321 gctacgcctg gacggcctat accttctcct gcaaccccgg cggcggcacg gtcaactgcg
     4381 ttcgttgggc caagatcgac ccggccacgg cgaccatcgc cgacgccggc gtgttggcca
     4441 cagccggaca gcatcgcatc ttccccaacc tggccgtcaa ccgctgcgac gatatggctg
     4501 ttggctacac gaagaccagc gcgtcgagct tccccggcgt cttcgccacc ggccgccagg
     4561 ccagcgaccc cgccggcacg ctccaggccg agacccaact ccaggccggc accatcacct
     4621 ataccgcctt cgacggctcg ccccaccgct ggggcgacta caccggcatg accatcgacc
     4681 ccgacggcga gacgttctgg tatctggggg agtacagcaa gaacaccggc accagcaacg
     4741 gccgctgggc cacctacgtc cgctccctgg cctatgagac ctgtggcggc ggccccggcc
     4801 cgacgcaact cagcgtcgac ctgtcggcca aagccgccgg cgcggcgggc ggcgtcgcct
     4861 ttgccgccaa tgacatcatc cactacgacg gcgcgactga cacctggtcg atgcttttcg
     4921 acgcctccga cgtcggcatc accaaaaacg tcaccgcctt ctatcgccag gacaacgccg
     4981 ccacgctcga tacgttctat ctcgtgctgg cggccaatca gaacgtgccc ggcgtgggca
     5041 cggtgacgcc caacgatgtt attcgcttca cgcccacctc gctgggcagc agcaccgcgg
     5101 gcagcttcgc tctctatttc gacggctccg acgtcggcct gacaaccacg gccgagcaca
     5161 tcgacgccct gggcatggac ggcagccggc tgctcatcag caccaccggc gcggccaagg
     5221 ttccgcgcgg cggcggcggc agcctcaacg cgcccgacga ggacgtgatc gccttcacgc
     5281 cgaccagcac cggggcgacg acggcgggca cgtgggcggc cttcttcgat ggttcgaccg
     5341 tgcccggcct ggcggtcgag gacgtagctg gcttctggga caatcccgcc aacggcaacc
     5401 tggtcatctc ggtgctcaat gcctttaatc tgagcggcgt caccggcgat aacgacgacc
     5461 tcatcggcct gacgccctcc ggcggcagct acacgccgtc catcgtctgg aacggcgacg
     5521 acgcgggctt taacgtcgcc atcgacgctt tcgagattgt gccgtagtca attcacggct
     5581 tttttgccgt cgctgtggct gccgttctct ataatggcac tatcggccgg cctccgccgc
     5641 tgcttcgcga tcctgcgggc caggcggagg ccgcggccgg gatacaggtg ctatgtccag
     5701 tcgcggtcgt ccactctctc tcctcctgcc gatggccctt gtcgccctgg cggcccttct
     5761 ggcgttggcc gcgcgcggcg tcctagcgcg gcccctgacc gaaccagccc gcccggcggc
     5821 cgcgcccgtg ctggccgtct ccgactacac aactgccacc gccggccagc cggtggttgt
     5881 gccgctggcc ttcagcagcg gcggacacga catcgacacc atcttcttcg tcctgtccta
     5941 cgacgccacc ctgctgacgc tggacaaaac cgacgccaac gacgacggcg tacccgacag
     6001 catcgtcccg acgctgcccg gtggctaccg gctgatcgcc tccatcgaca tcgccggcgg
     6061 cgaggggcgg ttccaggcgc tcatctaccc cagccccgat agcccgccgc tgcccagtgg
     6121 caacctggcg acgctgacct tcaaggtgcc ctggcggccg acgcccgtgc cgacctatct
     6181 cgacttcctc acatcgcccg cgcccttctt cgccaacacc aatgatcagg tcgtgcccgg
     6241 cagctttgtc ggcggcatgg tggacatcaa ccagattccc acgctgacac cgacaccctc
     6301 gcgcacgccc acgcccacgg ccacgccctc gcgcacgccg acagccacgc cgacgacgac
     6361 gccgacggcg acgaccacag gcacggccac gccgaccgtc accggcacgc cgccgacgcc
     6421 aacgccctcg ccctcgccta cagtaacggc cacagccagt gccacggcca cagccagtgc
     6481 cacggccagt gccacgccga cggccgcgcc catctatttg ccggcgctgc tctatggcgc
     6541 gtcgcccacg ccgacggcga cagcgtcgcc cacgccgacg gcgacgccca ctgccagcgc
     6601 aacactcacg tccacgccca cagcgtcgtc cacgcccacg gccaccatca ccggtacgcc
     6661 gccaacgccg gcgagcacgc caacaccctc gcgcacgccg acggc
//
LOCUS       DAOSMQ010000128         6686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11520_length_6686_cov_4.489971, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000128 DAOSMQ010000000
VERSION     DAOSMQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11520_length_6686_cov_4.489971"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PK829_05735"
     CDS             complement(<1..230)
                     /locus_tag="PK829_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOU40749.1"
                     /translation="MTIATAPAVRAVSVALAGNPNAGKTTLFNALTGARQHVGNWPGK
                     TVEKKTGILVRDALRLDITDLPGAYSLSAYSL"
     gene            complement(270..692)
                     /locus_tag="PK829_05740"
     CDS             complement(270..692)
                     /locus_tag="PK829_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOU40750.1"
                     /translation="MPTLSSTPWPAMWVLLTAFLAAGLILLIALSPVESRRRLLGRRA
                     AQPVVVDERVEEFLAAGLLPLSDCPRDRTACLRCLQVDRCIVHRLLELGLTPGVELRV
                     VQDNGGPMLISVRGSRVALGRDLADNLWVEMDSRADGK"
     gene            complement(692..1363)
                     /locus_tag="PK829_05745"
     CDS             complement(692..1363)
                     /locus_tag="PK829_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOU40751.1"
                     /translation="MKHDSFHESAEMYLKTASELAGCNAPTPISTLAGRLGVSAVSAT
                     EMVHRLAEQGLLAHQPYRGISLTETGQQLADEVVRRHRLWECFLYERLGLPWHEVHDH
                     ACRLEHATTPAVTEALDAFLGHPVACPHGNPICGRAGAPAERPLSELPVGGTAVLVRV
                     HPESESLLCYLAELDLLPGRRVTLREIAPYQGPLVLLTDVGVRYIGREAAAHVHIRLL
                     TEDPA"
     gene            complement(1493..2221)
                     /locus_tag="PK829_05750"
     CDS             complement(1493..2221)
                     /locus_tag="PK829_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU40752.1"
                     /translation="MMAAPFDTADDHNAWLTLSQAAHFLDVHPTTLRRWANNGDIPAM
                     VTPGGHRRFAATDLARFARERSALRNVQGIAGLWATKALAHTRQEVNNHRADSWLARY
                     DEKERDHNRPLGQQLMGLTLQYLSSESSEDNSASILEEAKRIGRLYAHNALAQGLSLR
                     ATLEASIFFRDKLVETALQLPETANIRPEANMRLLRRVNELLNAVHLAAVEVFENGHS
                     TNDMPDAAVAGDPPPANPPDAPTS"
     gene            complement(2336..>2641)
                     /locus_tag="PK829_05755"
     CDS             complement(2336..>2641)
                     /locus_tag="PK829_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU40753.1"
                     /translation="LDVPVVIALAANQEGDEQRRREDYAAVCCAAQNVQLAAWAAGIG
                     TKWSTSGVTRDPLAYELLGLDPARFDIIGFLYAGYPAETPAARPRRLALDEVIRTTS"
     assembly_gap    2642..2651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2652..2926)
                     /locus_tag="PK829_05760"
     CDS             complement(<2652..2926)
                     /locus_tag="PK829_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU40754.1"
                     /translation="MDVLEAIATRRTIAKFQPEPIPPELLRAALAAGVWAPNHRLTEP
                     WRFIILGPETQRRLAARFGELKAAKIVTESAERRERLGRRRRHDDHPR"
     gene            complement(2929..4110)
                     /locus_tag="PK829_05765"
     CDS             complement(2929..4110)
                     /locus_tag="PK829_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU40755.1"
                     /translation="MSANGHNSINFCLSDEHRDIQRLARDFARKEIAPQAEHYDKTHE
                     YPWPIIREAQKIGLTTMAVPEQYGGLGLSLFEECLVTEELAWGCSGVSTAIGVNGLGV
                     LPIMIAGTEEQKMEYGGRMAEGELAAYCVTEPEAGSDVAGIQTTAIRDGDHYVLSGGK
                     TFITGATNANFYSVLAYTNPAVREDRRQRYTGMSFFVVERGWDGISVGKPFEKMGQHA
                     SDTAEVIFDNVRVPISYRLGEEGSGFATTMKVFDKSRPSVAAGAVGVAQRAMDEAIKY
                     AKERMTMGQPIWQHQAIGHMIADMAMQIEAARMLVYRAAWDYDHGDRNTIHAAMAKAY
                     AADAAMKVTTDAVQVFGGYGFMSEYPVEKLMRDIKIYQIYEGTSQIQRNIIVRELFRS
                     R"
     gene            complement(4304..5113)
                     /locus_tag="PK829_05770"
     CDS             complement(4304..5113)
                     /locus_tag="PK829_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40756.1"
                     /translation="MRHLLFLAATLLAALTMTGVRAASPAAVTPPDQFYLPIVLSQPL
                     APPYDMVQFMVGDHRLYEVQHSSGSQARHQTQETRTRFYHTKGDEVRAEWEELWATAD
                     TIFRGTDTSPGNGQYYTLYEDDVAGSAWSPRLWDVGDVYERNPFVVFYRKSDCSVVAS
                     GYQRSWLRFEAYYPTYTFRSGISLRNVIELAWLLKPDGQPVESYFYAEKYGLVGWGSV
                     DHGYSYISWVHQPGQRPDNKREVIACLDANGLLRGKGALNRGPLPEGFRAK"
     gene            5259..6500
                     /locus_tag="PK829_05775"
     CDS             5259..6500
                     /locus_tag="PK829_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HOU40757.1"
                     /translation="MNAFSNLNALRAQFPALQRTVGSQPAVFLDGPGGTQSPVRVIDA
                     MSDYLRRGSSNHGGHFLTSRESDATAEAARLAMRDLLNAHRPEEIIFGQNMTSLTFSL
                     SRALAQTWQPGEEIIVTRLDHDANISPWLRAAADRGVTVRWLDFHPADCTLALEELPG
                     LLNERTRLVAVNYASNAVGTISDVGRVVALAHAAGALVYVDAVHYTPHDVVDVQALDC
                     DFLACSVYKFYGPHTGVVYGKYEWLDRLTAYKVRPAADAPPGKWETGTQSYESLSGVA
                     AAVDYLAELGGAGGSRREQLARGMAAIKAYEVTLSEHFLRGAATVPGLRVYGITDIEM
                     LARRTPTFAVELAGRAPAEVAAWLGERGFFVWDGHYYAIAVMERLGTLERGGLVRIGF
                     VHYNTIAEIDRLLAALRELAD"
BASE COUNT         1127 a         2133 c         2218 g         1198 t
ORIGIN      
        1 tccagcgaat aggcactgag gctataagcg ccgggcaggt cggtgatgtc caaccgcagc
       61 gcgtcacgca ccagaatgcc ggtcttcttc tcgaccgtct tgcccggcca gttgccgaca
      121 tgctgccgcg cgccggtcag ggcgttgaaa agggttgtct tacccgcgtt gggattgcca
      181 gccagggcga cggagacggc gcgcacggcc ggcgcggttg caatggtcat catgggagga
      241 tcggtaaggg tttgcggagg ggatgggcgt cacttgccgt cggcccggct gtccatctcc
      301 acccacagat tgtcagccag gtcgcgtccc agggccacgc gcgaaccgcg cacggagatc
      361 agcattggcc cgccgttgtc ctggacgacg cgcaactcga cgccgggcgt caggcccagt
      421 tccaacagcc ggtggacaat gcagcggtcg acctgcaagc agcgcagaca ggccgtccgg
      481 tcgcgagggc aatcggacag gggcagcaag ccggcggcca gaaactcttc cacgcgctca
      541 tcgaccacga cgggctgggc cgcgcggcgg cccaagagac gacggcggct ctcgaccggc
      601 gacagagcaa tcagcaggat gagaccggcg gccagaaagg cagtcagcag cacccacata
      661 gcgggccatg gtgtggacga cagtgttggc atcaggcggg atcctccgtc aagaggcgga
      721 tgtggacgtg ggcggcggcc tcgcggccaa tgtagcggac gcctacgtcg gtcagcagaa
      781 ccagcggccc ctggtagggc gcgatttcgc gcaaagtgac gcgccggccg ggcagcaagt
      841 ccagttcggc caggtagcaa agcaagctct cgctttccgg gtggacgcgg acgaggacgg
      901 ccgtgccgcc gacgggcaat tcgctcaacg ggcgctctgc cggcgcgccg gcccggccgc
      961 aaatggggtt gccgtggggg caggcgacgg gatggcccag aaaagcgtcc agcgcttcgg
     1021 tcacagccgg cgtggtggca tgctctagcc ggcaagcgtg gtcgtggact tcgtgccagg
     1081 gcaggccgag ccgctcatag aggaagcact cccataggcg gtggcggcgc acaacctcat
     1141 cagccagttg ctgtcccgtc tcggtcaggc taatgccgcg gtagggctgg tgagccaaca
     1201 acccttgctc cgccaggcgg tgaaccatct cggtggccga gacagccgag acgcccagac
     1261 ggccggccag cgtggagatg ggcgttggcg cgttacagcc ggccagttcg ctggctgtct
     1321 tgaggtacat ttcggcgctt tcgtggaagg aatcgtgttt catggcggtt gcgcccgcgc
     1381 cgggccggca ggataaaggg tttaccgcgc cccggttttt gggtcaacct tatattagta
     1441 gagtggctcc cctatgaata gtgacatctg tcacccctta aggtgacatc cgtcacgagg
     1501 ttggcgcgtc gggcgggttg gccggcggag ggtcgccggc gaccgcggca tcgggcatat
     1561 cgtttgtgct gtggccgttc tcgaagacct ccaccgcagc cagatggacg gcgttgagca
     1621 actcattgac gcgccgcagc aggcgcatgt tggcctccgg gcgaatgtta gccgtctccg
     1681 gcagttgcaa ggctgtctcg acgagcttgt cgcgaaagaa gatcgacgct tccagcgtgg
     1741 cgcgcaggga taggccctgg gccagggcgt tgtgggcata gaggcggccg atgcgcttgg
     1801 cctcctccag aatcgacgcg ctattgtcct cgctcgattc gctggaaagg tactgcaacg
     1861 tcagtcccat gagttgctgg ccgagtgggc ggttgtggtc gcgctccttc tcgtcatacc
     1921 tggccagcca gctgtcggcg cgatggttgt tgacttcctg gcgcgtgtgg gccagagcct
     1981 tggtggccca caacccggcg atgccctgca cattgcgcag ggcgctgcgc tcgcgggcga
     2041 agcgggccag gtcggtggcg gcaaagcgcc ggtggccgcc gggcgtcacc atcgcgggga
     2101 tgtcaccatt gttggcccag cgccgcagcg tagtcggatg cacatccagg aaatgcgccg
     2161 cctggctcag ggttagccag gcgttgtgat catcagcggt gtcaaacggg gcagccatca
     2221 ttaatctatg caatcctgag cgattgtgac ggctaatgct actcgactgg agcgcgggcg
     2281 tcaaatggac aaccagcaga gtgaccggcg tcccggtcac cccgctggcc atgcctcagg
     2341 aggtcgtgcg aatgacttcg tccagcgcca ggcggcgcgg gcgcgccgcc ggcgtctcgg
     2401 ccgggtagcc agcatagagg aagccgataa tatcgaagcg cgccgggtcc aggcccaata
     2461 gttcataggc caaaggatcg cgcgtcacgc cgctggtgct ccacttcgtg ccgatgccgg
     2521 cggcccaggc ggccaattgg acgttctggg ccgcgcaaca gacggcagcg taatcctcgc
     2581 ggcggcgctg ctcgtcgccc tcctggttgg ccgccagggc gatgacgacg ggaacatcca
     2641 gnnnnnnnnn ncgcgggtgg tcgtcatggc gccggcggcg gccgagacgt tcgcggcgct
     2701 cggccgactc agtgacgatc ttggccgctt tcagctcgcc aaagcgcgcc gccagccgcc
     2761 gctgtgtctc cggccccagg atgatgaacc gccacggctc cgtcaggcgg tggttgggcg
     2821 cccacacacc ggcggccagc gccgcccgca acagctccgg cggaattggc tccggctgga
     2881 acttggcgat ggttcggcgc gtggcaatcg cttcgagaac gtccatgcct aacggctgcg
     2941 gaacagttcg cggacgatga tgttgcgctg aatctggcta gtgccctcgt agatctgata
     3001 gatcttgatg tcgcgcatca gcttttcgac cgggtactcc gacatgaaac catagccgcc
     3061 aaagacctgc acggcgtcgg tcgtgacctt catggccgcg tcggcggcat aggccttggc
     3121 catggccgcg tggatggtgt tgcggtcgcc gtggtcgtag tcccacgccg cccggtagac
     3181 cagcatgcgt gccgcctcga tctgcatggc catgtcggcg atcatgtggc cgatggcctg
     3241 gtgctgccag atgggctggc ccatcgtcat gcgctccttg gcgtacttga tggcctcgtc
     3301 catcgcccgt tgggccacac cgacagcccc cgcggccacg ctcgggcggc tcttgtcgaa
     3361 gaccttcatc gtcgtggcga agccgctgcc ttcctcaccc agccggtagc taatgggcac
     3421 gcgcacgttg tcgaagatga cctcggccgt gtccgacgcg tgttggccca tcttctcgaa
     3481 cggcttgccg acgctgatgc cgtcccaacc gcgctcgacg acgaagaagc tcatgccggt
     3541 gtagcgctgg cggcggtctt cgcgcacggc cggattggta taggccagca cggaatagaa
     3601 gttggcattg gtggccccgg tgataaaggt cttgccgccg ctaaggacgt agtggtcgcc
     3661 gtcgcgtatg gccgtcgtct ggataccggc cacgtcggaa ccagcctccg gctcagtgac
     3721 gcagtaggcc gccagttcgc cctcggccat gcgcccaccg tattccatct tctgctcttc
     3781 cgtcccggcg atcatgattg gcagtacgcc caatccgttg acgccgatcg ccgtgctgac
     3841 gccggaacag ccccaggcca attcttccgt caccaggcac tcctcaaaca ggctcagccc
     3901 caggccgcca tattgctccg gcacagccat cgtcgtcagg ccgatttttt gggcctcgcg
     3961 aatgatgggc caggggtact cgtgagtctt gtcgtagtgt tccgcctggg gcgcgatctc
     4021 cttgcgggcg aagtcgcggg ccaggcgttg aatgtcgcgg tgctcgtcgc tcagacagaa
     4081 gttgatcgaa ttatggccat tggcgctcat ggtgcggtct cctatttaca ggcgtggtga
     4141 taacgggatt ggcgcggatg gtccaggcag cccctggccg caggtggcca ttgccgcagg
     4201 cattccatca ttgtttggcg ccgcgcgtcc ggcgcagcgt gtcctataac gcaacgcgtt
     4261 atctagtcat tctagcccac gcccggcagc gcgtcaagtg cgatcacttg gcgcggaagc
     4321 cctccggcag cgggccgcgg ttaagcgccc ccttgccacg cagcagcccg ttcgcgtcaa
     4381 ggcaagcgat gacctcgcgc ttgttgtccg gccgctggcc cggctggtgc acccaactga
     4441 tgtagctgta accgtggtcc acactgcccc aaccaacaag gccatacttc tcggcataga
     4501 agtagctctc cacgggttgg ccgtcgggct tcagcagcca ggccaactca atgacgtttc
     4561 gcaggctgat gccactgcga aacgtgtagg tgggatagta ggcctcaaag cgcagccaac
     4621 tacgctggta gccgctggcg acgacgctgc aatcgctctt gcggtagaag acaacaaagg
     4681 ggttgcgctc gtagacatcg ccgacgtccc acaggcgcgg actccaggcc gaaccagcaa
     4741 cgtcgtcctc gtagagggtg tagtactggc cgttgccagg gctggtgtcg gtgccgcgga
     4801 agatggtgtc ggccgtggcc catagctcct cccactcggc gcgcacctcg tcccccttgg
     4861 tgtggtagaa gcgggtgcgc gtctcctggg tttggtggcg ggcttgcgag ccgctgctgt
     4921 gttgcacctc atagaggcga tggtcgccga ccatgaactg gaccatgtca tagggcgggg
     4981 ccaggggctg gctgaggacg atgggcagat agaattgatc gggcggcgta acggccgccg
     5041 gtgaggcggc gcgaacgccg gtcatcgtga gcgcggccag cagtgtggcc gccagaaaga
     5101 ggaggtgtcg catcattgtt tccgcgcggc gggccggagc actggtgttt gtccaggcca
     5161 gatcatgggc catcttgccg cccgcgtcag ctatccgacg cagttcttca gtgaccattc
     5221 atacagtata atggctgcga cttcaacagg agcaaaccat gaacgctttc tccaacctga
     5281 atgcccttcg cgcccaattc ccggccctgc aacgcaccgt cggcagccag ccggccgttt
     5341 tcctcgatgg tcccggcggc acgcaatcgc cggtgcgggt catcgacgcc atgagcgact
     5401 atctacgccg cggcagcagc aaccacggcg gccacttcct gaccagccgc gagagcgacg
     5461 ccacggccga ggcggcccgc ctggccatgc gcgacctgct taacgcccac cgtccggagg
     5521 agatcatctt cggccagaac atgaccagcc tgaccttcag cctcagccgc gcgctcgccc
     5581 agacctggca gccgggcgag gagatcatcg ttacgcgcct tgaccacgac gccaatatct
     5641 ctccctggct gcgcgccgcc gccgaccgcg gcgtgacggt gcgctggctc gacttccacc
     5701 cggccgactg caccctggcc ctggaggagt tgcccggtct gctgaacgag cgcacccgcc
     5761 tggtggcagt caactacgcc tctaacgccg tgggcacgat cagcgatgtt ggccgcgtcg
     5821 tcgccctggc ccacgccgcc ggggcgctgg tctacgtcga tgccgtccac tacacgcccc
     5881 acgacgtggt cgatgtgcaa gcgctcgatt gcgacttcct cgcctgtagc gtgtacaagt
     5941 tttacggccc ccacacgggc gtggtatacg gtaagtacga gtggctcgac cgcctaacgg
     6001 cctataaggt gcgccccgcg gccgacgcgc cgcccggcaa atgggagaca ggcacgcaga
     6061 gctacgaaag cctcagcggc gtggcggcgg ccgtggacta tctggccgag ttgggcgggg
     6121 ccggcggctc gcggcgcgag cagttggcgc ggggcatggc agccatcaag gcctatgaag
     6181 tgacgctgag cgagcatttc ctacgtgggg ccgcgaccgt gcccggcctg cgcgtctatg
     6241 gcatcaccga cattgagatg ctggcccggc gcacaccgac cttcgccgtc gagctggccg
     6301 gccgcgcgcc agccgaggtc gccgcctggc tgggagagcg tggtttcttc gtctgggatg
     6361 gccactacta cgccattgcc gtcatggagc ggctgggcac gctggagcgc ggcggcctgg
     6421 tgcgcatcgg cttcgtccac tacaacacca tcgcggagat cgaccggttg cttgccgccc
     6481 tgcgcgaact ggccgattga tggcaacatg agcgctgatt gacagcggcg gtatattctg
     6541 tcataatctg cgacatcatc ttattgattg gcggcccacc cgcccgctat ctttaactaa
     6601 aggaggtggt gtctatgaat agttgcttat ctaactgcac cgtagcactg cctcctcgtg
     6661 agagctagta gtgctacggt gcaaag
//
LOCUS       DAOSMQ010000129         6667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11562_length_6667_cov_3.686177, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000129 DAOSMQ010000000
VERSION     DAOSMQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11562_length_6667_cov_3.686177"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PK829_05780"
     CDS             complement(<1..787)
                     /locus_tag="PK829_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOU40758.1"
                     /translation="METTPLTTTARKRLNLSNLLIGRPLETRTLAHQVVSKKVGLAVF
                     ASDAISSTAYATEEILVILALAGAGAFGLSVPIAMAIAILLVIVTVSYRQTIFAYPNG
                     GGAYIVARDNLGEVAAQIAGAALLTDYILTVAVSVSSGVAQIVSAFPGLVPLRVVIAV
                     SVIAFMTLINLRGVRESGSIFAIPTYFFLGMMFLTLGIGFFRWATGTLPTVSGVEQVA
                     VQSLAPLGLFLILRAFSSGSTALTGIEAISNGITAFKEPRSRNA"
     gene            complement(834..1286)
                     /locus_tag="PK829_05785"
     CDS             complement(834..1286)
                     /locus_tag="PK829_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40759.1"
                     /translation="MTLPILVVTGEPAWTMQALHLAAAMAREAGAPIILVEMIPASHI
                     QSLGAEPDETLLPLARMESLRQYADAVEGYGITVTVATFQCADYLGGLLSACEQASAT
                     AVFAPAPGGALRFLAAARLWYLRRALRRPLFTLDDGRAAVWQPRPLGA"
     gene            complement(1404..2096)
                     /locus_tag="PK829_05790"
     CDS             complement(1404..2096)
                     /locus_tag="PK829_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU40760.1"
                     /translation="MMILIVDDEPQIRKLLTAGLKGYGYDVLAAADGHEALTLVAQRR
                     PELVVLDIHLSQGPDGLEVCRRLREWSRVPIIMLSVRGDERTKVQALDAGADDYLTKP
                     FGMEELRARIQAVVRRAVAEPGAPPDSVIRAGALAIDLVNRRVTVAGEEVHFTPQEYD
                     ILRLLATHPGKIMTHPAILSAVWGPEYADMTHYVRIYVNQIRKKLRENPAANVRYILN
                     EPGVGYRFIDLP"
     gene            complement(2093..3535)
                     /locus_tag="PK829_05795"
     CDS             complement(2093..3535)
                     /locus_tag="PK829_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOU40761.1"
                     /translation="MTATLRPAASPDRRAALLRAAGEYLAAAALVVATTLALWPLRDR
                     LTIANESLLYMLVTLIVAVRFRTGPSVLAALLSFFAFNYFLVRPYYTLAVEDTRELLD
                     LLVFLTAAVIAGRLTDYARHQAEAAGLNARQQDILYRLTSALNPLTDVDAIRAELRRV
                     AVEELGAVEVEFLPMPPTAPTEHHPAADVVFVLLQAGEAIYGTLRASFLRAPSTSAHR
                     LLIACAGQAALALQRVDLTAQAQRSRALGEADRLKTALLRAVSHDLRTPITIIKTSAA
                     NLGDVDLAARLSAEEKGDMVQAIEASADALDRLVGNLLDMSRLQAGAVVLNEDWNSVE
                     EIAGDVAAGVYRREGTERIRLDFPDDLPLVRCDFGLLRQALGNLVDNALRHEPEGQRV
                     VIRGRAEAGLVRLEVVNHGPTIPAEERERIMEPFYQSRDGRSVMGGVGLGLAIAHGIA
                     AVHGGALAVADTPGGGATFIIRLPQTEGAP"
     gene            complement(3802..4584)
                     /locus_tag="PK829_05800"
     CDS             complement(3802..4584)
                     /locus_tag="PK829_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOU40762.1"
                     /translation="MTKDSRSISIITLVVVIGATIAANVLAFTTNMDTGDIANSTFDS
                     TNYFFPATYVFTTIWPVIYASIVGWAIYQALPAQRDNPRFRAAAPWLIVNMGLNAVWV
                     WIFGMQAFVTTVAVIIPLLYTTVMVYRKLRVGEVKVGTWERVFQVGITIYLAWLCVAT
                     VANVASALISVGWNGFGLSREVWGLIMLLVGTGLAFFLYRGFKREVVIPLVFIYAYAG
                     IIARYADEPLILTGAILGAVALAGLSLFHFIGGGRARALQPA"
     gene            4870..5352
                     /locus_tag="PK829_05805"
     CDS             4870..5352
                     /locus_tag="PK829_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOU40763.1"
                     /translation="MAHVVHIGFQPLDRDYEDRMGDYIRVPVDSALLVAGHGIRGDRK
                     AGRNQTRQVNLIAREWLAARAEEGYRAAPGQFGEQIVIGGLAVETLPPETLLAIGEEV
                     VLAVSKGRTGCERLEAAQGKSIAGLGPIGVLTRVVHGGRIYVGDEVRVLQPAEAQANG
                     "
     gene            5349..6083
                     /locus_tag="PK829_05810"
     CDS             5349..6083
                     /locus_tag="PK829_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40764.1"
                     /translation="MIDDNVRASLCAGNNAHWCDAVGRAYGRPGEFRPTVWLNHAAMP
                     RDYPNAVTLTPGGAATQLAAVHDLVAARPTCSVKDSFATLDLRSLGFDVLFEAAWLWR
                     AAYAPAPAASSLDWAIVSDAATLARWEAVWAGQGEPPADSERIFPPALLRESGILFMA
                     GLRARQIVTVAVATVAGTAIGLSNVVASDGDTEAAYAGAVALLSVLSPERALVGYERG
                     DALTAALRVGFEPVGPLRVWARQGTP"
     gene            6136..>6667
                     /locus_tag="PK829_05815"
     CDS             6136..>6667
                     /locus_tag="PK829_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOU40765.1"
                     /translation="MDKAIRDRYSDAIVQAVAARYGTTPAALAALGGFESFIYRFDYG
                     GMPAILRVTHSLRRSPEMIHGEVDWINALAAGGATVARALLSARGELVEEIDDGAGGR
                     FLATAFAFAPGRPPWEVGWTPARTDAYGRLLGRMHALTKAYAPADPTWRREAWPAAFE
                     AEIAGLLGGVDEPLLAR"
BASE COUNT         1092 a         2084 c         2456 g         1035 t
ORIGIN      
        1 cggcgttgcg gctgcgcggc tccttgaagg cggtgatgcc gttggagatg gcctcaatgc
       61 cggttagcgc cgtggaaccg ctggagaagg cccgcagaat caggaatagc cccagcggcg
      121 cgagcgactg gacggccacc tgttcgacgc cgctcacggt cggcagcgta cccgtggccc
      181 aacgaaagaa gccaatgccc agcgtcagga acatcatgcc caggaagaag taggtcggaa
      241 tggcgaagat gctgccactc tcgcgcacgc cgcgcaggtt gatgagcgtc atgaaggcga
      301 tcacgctgac ggcgatgacc acgcgcaagg gcacaagacc cggaaaggcc gagacgatct
      361 gggccacgcc ggaggagacg gacacggcaa cggtcaggat gtagtcggtt agcagggccg
      421 cgccggcgat ctgcgcggcg acttcgccca ggttgtcgcg ggcgacgatg taggccccac
      481 cgccgttggg gtaggcgaag atggtctgcc ggtaggagac cgtgacgatg accagcagaa
      541 tggcgatggc catggcgatg ggcacggaca ggccaaacgc cccggccccg gccagggcca
      601 ggatgaccag gatttcctcg gtggcgtagg ccgtcgagga gatggcgtcg gacgcaaaga
      661 cggccaggcc gacctttttg ctgacgacct ggtgggccag ggtacgcgtt tccagcggcc
      721 ggccgatgag caggttagag agattgaggc gctttcgcgc ggttgtggtc agtggtgttg
      781 tttccataga actaccttgc tgaatggggg ccgccgcggg tttggcgcgg cgactaggcc
      841 cccaggggac ggggctgcca gacagcggcg cggccgtcat ccagcgtgaa cagcggccgg
      901 cgcaaagccc gccgcagata ccagagtcgc gcggcggcca ggaagcgcag cgcgccgccc
      961 ggcgcggggg cgaaaaccgc tgtcgcgctg gcctgttcgc aggcgctcag caggccaccg
     1021 aggtaatcgg cgcactggaa ggtggccacc gtcacggtga tgccgtagcc ctcgaccgcg
     1081 tcggcgtact gtcgcagcga ttccatgcgt gccagcggca agagggtctc gtccggctcc
     1141 gcgccaagcg actgaatgtg gctggcgggg atcatctcga cgagaatgat gggcgcgccg
     1201 gcctcgcggg ccatggccgc ggccaggtgt agtgcctgca tcgtccaggc cggttcgccg
     1261 gtgaccacaa ggatgggtaa tgtcatgctg tgctcctcgc ggggtcggac agactccact
     1321 aggcgacagt gtacgccggc ggccgtcaag aacgtgtcag gcaacacggg ccggggcgta
     1381 aggaatgcgt aaagacgggg cggctagggg aggtcgatga agcgatagcc cacgcccggc
     1441 tcgttgagga tgtagcggac gttggcggcc gggttttcgc gcagtttctt gcggatttga
     1501 ttgacataaa tccgcacgta gtgggtcatg tcggcgtatt ccggccccca gacggcgctg
     1561 aggatggctg ggtgggtcat gatcttgccg gggtgggtgg ccagcaggcg caagatgtcg
     1621 tattcctgcg gcgtgaagtg gacttcctcg cccgcaacgg ttacgcggcg gttgaccagg
     1681 tcgatggcca gcgcgccggc acggatgaca ctatccggcg gcgcgcccgg ctcggccacg
     1741 gcccggcgga caacggcctg aatgcgcgcg cgcagctctt ccatgccgaa tggcttagtt
     1801 agatagtcgt cggcgccggc gtcgagggcc tgcaccttgg tgcgctcgtc gccgcgcacc
     1861 gacagcatga tgatgggcac gcggctccac tcgcgcaggc ggcggcagac ctccagccca
     1921 tccggcccct ggctgaggtg gatgtcgagg acgaccagct cgggccggcg ctgggccaca
     1981 agggtcagcg cctcgtggcc gtcggccgcg gccaacacat catagccgta gcccttcagg
     2041 cccgcggtta gcagcttgcg gatctgcggc tcgtcgtcaa cgatgaggat catcatggcg
     2101 cgccttccgt ctggggcagc cggatgataa acgtggcccc gccgccgggc gtgtcggcga
     2161 cggccagcgc gccgccgtgg acggcagcga tgccgtgggc gatggccagc cccagcccga
     2221 cgccgcccat gaccgagcgg ccgtcgcgcg actggtagaa cggctccatg atgcgttcgc
     2281 gctcttcggc ggggatggtt gggccgtggt tgacaacctc caggcggacg aggccggctt
     2341 cggcccggcc gcggatgaca acccgttgcc cctccggctc gtggcgcagg gcgttatcaa
     2401 cgagattgcc cagcgcctgg cgcagcaggc caaagtcgca gcgcaccaga ggcaggtcgt
     2461 cggggaagtc caggcgaatg cgctccgtgc cctcgcggcg gtagacgccg gcggcgacgt
     2521 cgccggcgat ctcctccacc gagttccaat cctcattgag gacaaccgcg ccggcctgca
     2581 agcgggacat atccaacagg ttgccgacga ggcggtcgag agcgtcggcg ctggcctcga
     2641 tggcttgcac catgtcgccc ttctcctcgg ccgacaggcg ggcggccaga tcgacgtcgc
     2701 ccagattggc ggccgaggtc ttgataatgg tgatgggcgt gcgcaggtcg tgggaaacgg
     2761 cccgcaacag ggctgtcttt agccggtcgg cctcgcccag ggcgcggctg cgttgggctt
     2821 gagcggtcag atcgacgcgc tggagggcca gggctgcctg gccggcgcag gcgatcagca
     2881 gccggtgcgc gctggtcgag ggcgcgcgca ggaaggaggc gcgcagtgtg ccatagatgg
     2941 cctcgccggc ctggagtaaa acgaagacga cgtcggcggc gggatgatgt tccgtgggcg
     3001 cggtgggcgg catgggcagg aattccacct caaccgcgcc cagctcctcg acggccacgc
     3061 ggcgcaactc ggcgcggatg gcgtccacgt cggttagcgg gttgagggca ctggtcaggc
     3121 ggtagaggat gtcctgctgg cgggcattca ggccggcggc ttcggcctgg tggcgggcgt
     3181 agtcggtcag ccggccggcg atgacggccg cggtcaggaa gacgagcagg tcgagcaact
     3241 cgcgtgtgtc ctcgacggcg agggtgtagt agggccgcac gaggaagtag ttgaaggcga
     3301 agaaactgag cagggcggcc agcaccgatg ggccggtgcg aaagcggacg gcgacaatga
     3361 gcgtcaccaa catatagagc agcgactcgt tggcgatcgt cagccggtcg cgcaggggcc
     3421 agagggccag cgtggtggcg acgacgagag ccgccgccgc caggtattcg ccggccgcgc
     3481 gcaggagggc ggcgcggcgg tcggggctgg cggcggggcg caaggtagcg gtcatggatt
     3541 gcgggccggt ggacgccgtc tgggcgcgcc accatgccat cactatagcg ccgccggccg
     3601 cgggaggcaa cagcgccctg gtgcgagcat ggtttttcag ttgccggccc aggcgcgccg
     3661 acctgacagg tagcggcctc aggcctcgtc gctgtcagag tgccgttagc cgcttacctg
     3721 tcaggtcttt ggcctgggag gtgcgacgcg ctccagaaaa cgcgccgccc accggccggc
     3781 cccaccccaa cggcggcggc ctcaggccgg ctgcaacgcc cgcgcccggc cgccgccgat
     3841 aaagtggaag aggctcaacc ccgccagagc gaccgcgccg agaatggccc cggtcagaat
     3901 gagcggctca tcggcgtagc gcgcgatgat gccggcgtag gcatagatga acaccagagg
     3961 gatgactacc tcgcgtttga agccgcggta gaggaagaag gccagccccg tgccgacgag
     4021 cagcatgatc agcccccaga cctcgcggct caggccgaag ccattccagc cgaccgaaat
     4081 gagcgccgag gcgacgttgg ccaccgtggc gacgcacagc caggccagat agatcgtgat
     4141 gccgacctgg aagacgcgct cccaggtgcc aaccttcacc tcgccaacgc gcagcttgcg
     4201 atagaccatc acggtggtat agagcagcgg gatgatgacc gccacggttg tcacaaaggc
     4261 ctgcatgccg aagatccaca cccagacggc attgaggccc atgttgacga tgagccacgg
     4321 cgcggcggcg cggaagcgcg ggttatcgcg ctgggcgggc aaggcctggt agatggccca
     4381 gccaacgatg ctggcgtaga tgaccggcca gatggtcgtg aagacgtagg tggccgggaa
     4441 gaagtagttg gtgctgtcga aggtgctgtt ggcgatgtcg cccgtgtcca tgttggtcgt
     4501 gaaggccaga acgttggcgg cgatggtcgc gccgatgaca acaacgaggg taatgatcga
     4561 aatactgcga ctatccttag tcataatatg ctcctgccgc tgtgcggccg atgatgatcc
     4621 attaccatga taacggaaaa gagatggttt gtcaatgatt tgtccatatt tcatctaaag
     4681 ctgggcggcg tcgccgggtt gtggtggttt gccgtggctt gcgggtggct tgccgtggct
     4741 tgcgggtggc ttgccgtggc ttggcaaaag tttggttgtc ggccgccaat cgcctatact
     4801 tctgccggtc gcgcccaccg cagatccgct gccagactca gcgtcggggg cgcgccggag
     4861 ggatagtcca tggctcatgt cgttcatatt ggctttcagc cccttgaccg cgattatgag
     4921 gatcggatgg gcgattacat tcgcgtgccc gtcgattcgg ctttgctggt ggccggccac
     4981 ggcattcgcg gcgaccgcaa ggccggccgc aaccagacgc ggcaggtcaa cctgatcgcg
     5041 cgtgaatggc tggcggcgcg ggccgaggag ggctaccgcg ccgcgccggg ccagttcggc
     5101 gagcagatcg tcatcggcgg gctggcggtg gaaacgctgc cgccggagac gctactggcc
     5161 attggcgagg aggtagtgtt ggccgtcagc aaggggcgca ccggctgcga acggctggag
     5221 gcggcccagg gcaaatcgat tgccgggttg gggccaattg gcgtcctgac gcgcgtcgtc
     5281 cacggcgggc gcatctacgt gggcgacgag gtgcgcgtgc tccagccggc tgaggcgcag
     5341 gccaacggat gatcgacgac aacgtgcggg ccagcctatg cgcgggtaac aatgcccact
     5401 ggtgcgacgc tgtcggccgc gcctacggcc gacccggcga gttccgcccg acggtctggc
     5461 tcaaccacgc cgccatgcca cgcgattacc ccaacgccgt cactctgacg ccgggaggag
     5521 cggccaccca actggccgct gtccacgacc tcgtggcggc gcggccgacc tgctcggtca
     5581 aagacagctt cgccacactc gacctgcgtt cgctgggctt cgacgtcctc tttgaggccg
     5641 cgtggctgtg gcgcgcggcc tatgcgcccg cgccggcggc ctcgtcactg gactgggcga
     5701 ttgtgtccga cgccgcgacg ctggcgcgct gggaggcggt ctgggcgggg caaggcgagc
     5761 cgccagccga ctcagagcgc atcttcccgc cggcgctgct gcgcgagtcg ggcatcctgt
     5821 ttatggccgg cctgcgcgcc aggcagatcg tcaccgtggc cgtcgccact gtggccggca
     5881 cagcgattgg cctgtccaac gtggttgcgt cggacggcga cacggaggcg gcctatgccg
     5941 gagccgtcgc tctcctcagc gtcctctctc ccgaacgggc gctggttggc tatgagcgcg
     6001 gcgacgccct gaccgcggcg ctgcgcgtgg gctttgagcc ggtggggccg ctgcgggttt
     6061 gggcgcgcca ggggacaccc tgacgcgatg aacacggccg gcggctgagg gattctggcc
     6121 acggggaacc gccgtatgga caaagccatc cgcgaccgct acagcgacgc cattgtgcag
     6181 gccgttgccg cccgctatgg cacaacgccc gccgcgctgg cggcgctggg cggcttcgag
     6241 agcttcatct atcggttcga ctacggcggc atgccggcca tcctgcgtgt gacccactcg
     6301 ctgcgccgct cgccggagat gatccacggc gaagtggact ggatcaacgc cctggccgcg
     6361 ggcggggcga cggtggcgcg ggcgctgctg tcggcgcggg gggagttggt ggaagagatc
     6421 gacgatggcg cgggcggccg cttcctggcg accgcttttg cctttgcgcc gggccggccg
     6481 ccgtgggagg ttggctggac gccggcgcgg accgatgcct acggccggct gctgggccgg
     6541 atgcacgccc tgaccaaagc ctacgctccg gccgacccca cctggcggcg cgaggcgtgg
     6601 ccggccgcct ttgaggccga gatcgccggc ctgctgggcg gcgttgatga gccgctgctg
     6661 gctcgct
//
LOCUS       DAOSMQ010000130         6666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11566_length_6666_cov_3.909847, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000130 DAOSMQ010000000
VERSION     DAOSMQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11566_length_6666_cov_3.909847"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /gene="pabB"
                     /locus_tag="PK829_05820"
     CDS             <1..1585
                     /gene="pabB"
                     /locus_tag="PK829_05820"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOU40766.1"
                     /translation="REGLGEGAAEDPLPNPLPGGEGVCWEGTAEDPLPNPLPEGEGVR
                     LPAHRFGPWQHPLDFPAYAAALDAIKAAIARGDTYQANYTFPLRAGFEGDPWALFAAL
                     SAAQRADFGAYLDLGRFVICSASPELFFRLDGDRILSRPMKGTAPRGLTTAADRAQID
                     WLRRSEKNRAENVMIVDMIRNDLGRVAQVGSVAVPTLFAIERYPTLLQMTSTVTARTD
                     AALTDILAAAFPCASITGAPKKRTMALLTALEPQSRGVYTGAIGAILPGRRAQFNVAI
                     RTVVLDRARGAAVYGVGSGIVWDSDAAAEFEECRLKTRVLSVPPARPFRLLESLRWTP
                     AEGYALREEHLRRLADSAEYFGYPLALAATAARLDALAAGLAGPAKVRLLVDAAGQIE
                     AEARPLADGALPQPARLGLARQPVSSADVWLYHKTTRREVYEAARAGRPDCDAVALWN
                     ERGELTEATASNLVLVLDGRAWTSPVSAGLLAGTLRARLLAAGEITERTLTPGDLTRA
                     EGIWLINSVRGWQAARFVD"
     gene            1643..2188
                     /locus_tag="PK829_05825"
     CDS             1643..2188
                     /locus_tag="PK829_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU40767.1"
                     /translation="MTVPWREIIWQQFGAAIDDLGNAIRACPDDLWLHRLWVHQDEPP
                     ELAQVWYQVYHALFWVDIHLNGTEDGFLPPPPFELIEQDENGPIPARPYTKEELLGYL
                     AHCRRRCQTTIAGTTDEEAQRLCWMGWGEVSFAELLLYSMRHIAGHTAQLNLLLGQTP
                     GGAPGWVTRADRQRQPPHEDG"
     gene            complement(2201..3499)
                     /locus_tag="PK829_05830"
     CDS             complement(2201..3499)
                     /locus_tag="PK829_05830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU40768.1"
                     /translation="MTAVVPLTFTAIVLNWNGRAYLEACLSALLAQEYPIERLVVVDN
                     GSTDDSLAFLRQRFPAVPVLANGGNVGFAAGNNLALRNLTSAAALLLNPDVILSPGCV
                     GALAAALAADPAVAIAGGKLWYPDGQTLQHGGGFITRPQAMPGHYGIGEADAGQHDAT
                     RDVDYVIGAAVAVRRLALEQIGLFDEGFFLYYEDADLCARARAAGYRVVYEPRATAVH
                     VESAVAVRGSFSYYQRFHTGRWRYLLKHFSAGELLDETVPAEAAWLERVGGDERRAVS
                     LAYLATGHALADIRHARAANPLPDAAWTALAAALAQLGARARPRPEDAAALARLAAAA
                     VVVERPFVSNAPLLGPLIARFRTAWNNVASRWYVHHVVEQQNAFNQLAVRQLETYELE
                     LAEQMALLEEQIVEQEALRRRVAELAAQVAELRRRLAAAE"
     gene            complement(3496..4788)
                     /locus_tag="PK829_05835"
     CDS             complement(3496..4788)
                     /locus_tag="PK829_05835"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOU40769.1"
                     /translation="MWTDARQATAWAFVWLLLVTAGFVLLRPPAGGRRWPSWPIILLL
                     AALVRLLPALWLPVGAGYDIASFHMVTDALLGGREVYAAALGRHPYLPFQMFIMGGMA
                     ALSAATGLPFVVAIKLPALLADVAIAGLIYRLVASRRGRDWAAYAALLYALNPVSLLV
                     SAYHGQFEAVTLLLLVVAWALWERQRVGAAAAALGLAILNKTWPVVLLPVFLMRLATW
                     RARILHTLVALGIPALAVVVYLLAYNADPMTMLGRALTHRGVAGYWGPSAVVFPLATA
                     QPALQPLADALLALRNPLLLAAVLLTLWWTQRQPALDALLTVQLAVFAVTVGFGIQWL
                     VWPVPFALLAGQARWLRLYSLAAALMLAVHLFGLHMVPWLPERLPASADWIIRLSALP
                     AWGVVVAWLVARLRAAHAVAPAGGQHEDWPQSGGVAAP"
     gene            complement(4873..6612)
                     /locus_tag="PK829_05840"
     CDS             complement(4873..6612)
                     /locus_tag="PK829_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOU40770.1"
                     /translation="MKRSNVIHLFAGLVVVSAMLVLAFHALAAGIEASSDGLRAAETA
                     QQSDTPQAQEDILAATNCRYGVGYVPGLPHSLNWIPTLDAGWYVNFGASGGGPGVRSA
                     AFAPIIRVRQEQVNGVRTDNFIATPPMVFSYRNEQGALVNGLGRLVSSNPGGFWIIGN
                     EVDVNNYVQDNTMPQVYARAYHDIYQYIKRIDPTAKVAIAGLSMMTPGRLQYLSIVWD
                     TYRSVYGETMPVDVWNMHLYILEERNPADPNQYGDGKIALGTDPALAKLTSGGVADSC
                     PALGAADIPANDPRPDVHCRAEHDSVRIFREQVYAMRNWMKERGQQNKPLIISEFGLL
                     YPYVLDDNGQYFLKDEHGQPFAPARVTKYLQDTIAFLETAKDPNLGYPADGNRLVQQW
                     LWYSIVTGPETSGGSSNLISWDTFQNMPGGDPAALTMMGQAYQQAANASENWTNLVGG
                     VAQNVVTLAQGNNQSKALLTATFRNSGTLSVVAPIKVTFYRDQALTQEIGSAVYDPNA
                     RGAVAGCTWDGRNSKQVSVLWSNLSVGTHNYWAKIDTLNAVTETQETDNVTTRGTVTV
                     KPYGTFIPLANRQ"
BASE COUNT         1103 a         2209 c         2320 g         1034 t
ORIGIN      
        1 ccgggagggg ttaggggagg gcgcggcgga agaccctctc cccaaccctc tcccaggggg
       61 agagggagtc tgctgggagg gcacggcgga agaccctctc cccaaccctc tcccagaggg
      121 agagggggtc agattgcccg cccaccggtt cggcccgtgg cagcatccgc tcgacttccc
      181 cgcctacgct gccgccctcg acgccatcaa ggccgctatc gcccgcggcg atacctacca
      241 ggccaactac acttttcccc tgcgggccgg cttcgagggc gacccctggg cgcttttcgc
      301 cgccctcagc gcggcgcaac gcgccgactt cggcgcgtac cttgacctgg gccgtttcgt
      361 catctgttcg gcctcgccgg agttgttctt ccggctggac ggcgaccgca ttctctcccg
      421 gccgatgaag ggcacggctc cgcgcggtct gaccacagcc gctgaccggg cgcagatcga
      481 ctggttgcgc cggtcagaga agaaccgcgc cgagaacgtg atgatcgtcg atatgatccg
      541 caacgacctc ggccgcgtcg cccaggtcgg cagcgtggcc gtgcccactc tctttgccat
      601 cgagcgctac ccgacgctgt tgcagatgac ctcgaccgtc accgcccgca ccgacgccgc
      661 actgaccgac atcctggcgg ccgccttccc ctgcgcgtcg atcaccggcg cgcctaagaa
      721 gcggacgatg gcgttgctga ccgcgctgga accccagtcg cgcggcgtct acaccggggc
      781 cattggcgcg atcctgcccg gccgccgggc gcagttcaac gtcgccatcc gcactgtcgt
      841 gctcgaccgg gcgcggggcg cggccgtcta tggcgtcggc agtggcatcg tctgggattc
      901 cgacgccgcg gctgaattcg aggagtgccg gctaaagacg cgggtgctga gcgtgccgcc
      961 ggcgcgcccc ttccggctgc tggagtcgct gcgctggacg ccagccgagg gctatgccct
     1021 gcgcgaggag catctgcgcc gtctggccga ctcggccgag tacttcggct atccgctggc
     1081 cctggccgcc accgccgcgc ggctggacgc gctggccgcc ggactggccg gcccggccaa
     1141 ggtgcggcta ctggtcgatg ccgccggcca gatcgaggcg gaagccaggc cactggctga
     1201 cggggcgctg ccccaaccgg cgcggctggg cctggcgcgg cagccggtgt cgtcggccga
     1261 tgtctggctc taccacaaga cgacccgccg cgaggtctac gaggcggccc gcgccggccg
     1321 gcccgactgt gacgcggtgg cgctgtggaa cgagcgtggc gaactaaccg aagcgacggc
     1381 gtcgaacctg gtgctggtgc tcgacggccg ggcctggacg tcgccggtga gcgccgggct
     1441 gctggcgggc acgctgcggg cgcgcctgct ggccgccggt gagatcaccg agcgaacgct
     1501 gacgccaggg gatttgaccc gcgctgaggg catctggctc atcaattcgg tgcgcggctg
     1561 gcaggcggcg cgatttgtgg attaggagcc gccggcggcc gcgcgtcgtc gggccgatga
     1621 ccggcccaca aggagtagga tcatgacagt tccctggcga gaaatcatct ggcagcagtt
     1681 tggcgcggcc attgacgacc tgggcaacgc catccgcgcc tgccccgacg acctgtggct
     1741 gcaccgcctg tgggttcacc aggacgaacc gccggaacta gcgcaggtgt ggtatcaggt
     1801 ctatcacgcg ctattctggg tagatatcca cctgaacggc acggaagacg gcttcttgcc
     1861 gccgccgccg tttgaactca tcgagcagga cgaaaacggc cccattccgg cgcggcccta
     1921 caccaaagag gagttactgg gctatctggc gcattgccgc cgccgctgcc agacaacgat
     1981 cgccggcacg accgacgaag aggcccaacg tctgtgctgg atgggctggg gtgaggtgtc
     2041 gtttgccgaa ctgttgctct acagtatgcg ccacattgcc gggcacacgg cccaactgaa
     2101 tctgctgctg ggccagacgc cgggcggcgc gcccggctgg gtgacgcgcg ccgaccgaca
     2161 acgacagccg ccccacgagg acggctagcg aaccaggccg tcactcagcc gcagccagcc
     2221 ggcgacgcag ctcggccacc tgcgcggcca actcggccac gcggcggcgc agcgcctcct
     2281 gctccacaat ttgctcttct agcagcgcca tctgctcggc caactccaac tcataagtct
     2341 ccagttggcg gacggctaac tggttaaagg cattctgctg ctcgacgacg tggtggacgt
     2401 accagcgcga ggcgacgttg ttccaggcgg tacggaagcg ggcgatgagt gggccgagca
     2461 ggggggcgtt ggaaacgaac ggccgctcga cgaccacagc cgccgcggcc agccgcgcca
     2521 gggccgccgc gtcttccggc cgcggccggg cgcgcgcccc cagttgcgcc agcgccgcgg
     2581 ccagcgctgt ccaagccgcg tcgggtagcg ggttggcggc gcgagcatgg cgaatgtcgg
     2641 ccagggcgtg gcccgtggcc aggtaggcca gcgagacggc gcggcgctcg tcaccgccaa
     2701 cccgctccag ccaggcggcc tcggccggca cagtctcgtc cagaagctcg ccggcgctga
     2761 agtgcttcag caggtagcgc cagcggccgg tgtgaaagcg ctggtagtag ctgaagctgc
     2821 cgcggacggc cacagccgac tcgacgtgta ccgccgtggc ccgcggctcg tagacgacgc
     2881 gatagccggc cgcccgcgcc cgcgcgcaca gatcggcgtc ctcgtagtac agaaagaagc
     2941 cctcgtcgaa cagaccgatc tgttccagcg ccagccgccg gacggccacg gccgcgccga
     3001 tgacgtagtc cacgtcgcgg gtggcgtcgt gctgcccggc gtcggcctcg ccaataccgt
     3061 agtggccggg catagcctgg gggcgagtga tgaagccgcc gccgtgttgc agcgtttggc
     3121 cgtcgggata ccacaacttg ccgccggcaa tggctacggc cgggtcggcg gccagcgccg
     3181 cggccagcgc gcccacacag ccgggcgaga ggatgacgtc ggggttgagt agcagggcgg
     3241 cagcgctggt caggttgcgc agggccaggt tgttgccggc ggcgaagccg acgttgccgc
     3301 cgttagccag cacgggcacg gccgggaagc gctggcgcag aaaggccagt gagtcgtcgg
     3361 tcgagccgtt gtccacgacg acgagccgct caatggggta ctcctgggcc agcagcgccg
     3421 acaggcatgc ttccaggtag gcgcggccgt tccagttcag gacgatggcg gtgaaggtca
     3481 ggggcacgac ggcggtcatg gcgcggcgac gcccccgctt tgaggccaat cctcgtgctg
     3541 cccgccggcc ggggcgacgg cgtgggccgc ccgcagccgg gccaccagcc aggccacgac
     3601 cacgccccag gccggcagcg cgctcagccg gatgatccag tcggccgacg ccggcagccg
     3661 ctccggcaac cagggaacca tatgcaagcc aaagaggtga acggccagca tcagcgccgc
     3721 ggccagtgaa tagagccgca gccagcgagc ctgcccggcc agcagcgcga agggcaccgg
     3781 ccagacgagc cactggatgc cgaagccgac ggttacggcg aagacggcca gttgcaccgt
     3841 cagcagcgcg tccagcgccg gctggcgctg cgtccaccac agggtcagca ggacggcggc
     3901 gagtaacaac gggttgcgca gggccagcag ggcgtcggcc agtggttgta gggcgggttg
     3961 ggccgtggcc agcggaaaaa ccaccgcgct cggcccccaa tagccggcca cgccgcggtg
     4021 ggtcagggcg cggccgagca tggtcatggg gtcggcgttg taggccagca ggtacacaac
     4081 cacggccagt gccgggatgc ccagggcgac aagggtatgg aggatgcgcg cccgccaggt
     4141 ggccagacgc attaagaaga cgggcaacag gacgaccggc caggtcttgt tgaggatggc
     4201 cagacccaga gccgcggctg ccgctcccac tcgctgccgc tcccacagcg cccaggccac
     4261 gaccagtagc agcagcgtga cggcctcgaa ctggccatga taggccgaga ccagcaggct
     4321 gaccgggttc agcgcataga gcagggcggc ataggcggcc cagtcgcgtc cgcgccggct
     4381 ggccaccagg cgataaatca gcccggcgat ggccacgtcg gccagaaggg cgggcagctt
     4441 gatggccacg acgaagggca gacccgtggc ggccgacagc gcggccatgc cgcccatgat
     4501 gaacatctgg aagggtaggt agggatggcg gcccagggcg gcggcgtaga cttcgcggcc
     4561 gcccagcagc gcatcggtga ccatgtggaa cgacgcaatg tcatagcccg cgccgaccgg
     4621 cagccacagc gccggcagca agcggacaag agcggccaat agcaggatga tcggccagct
     4681 cggccagcgc cgcccgccgg ccggcgggcg cagcagcaca aaaccggctg tcaccagaag
     4741 cagccagacg aaggcccagg cagtcgcttg tcgcgcatcg gtccacatag agaaggagtc
     4801 tggcaatcaa ttttgccaga ctccttctct ttcgtccaat cgagcggcca ggccgcgccg
     4861 gcgtttggcc ctctactggc gatttgccag ggggatgaat gtgccgtagg gcttgaccgt
     4921 gaccgtgcca cgggttgtca cgttatccgt ctcctgtgtt tcggtaacag cgttgagcgt
     4981 atcgattttg gcccagtagt tgtgcgtgcc gacggatagg ttgctccaca gcaccgaaac
     5041 ctgcttgcta ttgcggccgt cccaggtgca acccgccact gcgccgcggg cgttggggtc
     5101 atagacggcc gagccgatct cctgcgtcag cgcctggtcg cggtagaacg tcaccttgat
     5161 gggcgcgaca acgctcagcg tgccgctgtt gcggaaagtg gccgtcaaca gggctttgct
     5221 ctggttgttg ccctgcgcca gggtgacaac gttctgggcg acgccgccga ccagattggt
     5281 ccagttctcg ctggcgttgg cggcctgttg ataggcctgg cccatcatcg tcagcgcggc
     5341 cgggtcgccg ccgggcatgt tctggaaggt atcccagctg atcaggttgc tggaaccgcc
     5401 ggaagtctcc ggcccggtga cgatgctgta ccacagccat tgctgcacca gccggttgcc
     5461 gtcggccggg tagcccaggt tagggtcctt ggctgtctcc agaaaggcga ttgtatcctg
     5521 caaatacttg gtgacgcgcg cgggggcaaa gggttggccg tgctcgtcct tcagaaagta
     5581 ctggccattg tcgtccagca cataggggta gagcaggcca aactcagaga tgatcagcgg
     5641 cttgttctgc tggccgcgct ccttcatcca gttgcgcatc gcatagacct gctcgcggaa
     5701 gatgcgcacg ctgtcgtgct ccgcgcggca atgcacatcc gggcgcgggt cgttggccgg
     5761 gatgtcggcc gcgccgaggg ccggacagct gtcggccacg ccccccgagg tcagcttggc
     5821 cagcgctggg tcggtgccta gggcgatctt gccgtcgcca tactggttag ggtcagccgg
     5881 gttgcgctcc tccaagatgt agaggtgcat gttccaaaca tcgaccggca tggtctcgcc
     5941 atagacgctg cgataggtgt cccagacgat ggacaggtat tgcaggcgac cgggggtcat
     6001 catcgacagg ccggcgatgg ctaccttggc tgttgggtcg atgcgcttga tatactgata
     6061 gatatcgtga taggctcgcg catagacctg cggcatcgtg ttgtcctgaa cgtagttgtt
     6121 gacgtcaact tcattgccga tgatccagaa gccgccggga ttgctgctaa ccagccgccc
     6181 caggccgttc acgagcgcgc cctgctcgtt gcgatagctg aagaccatcg gtggggtagc
     6241 gatgaagtta tccgtgcgca cgccgttgac ctgttcctgg cggacgcgaa tgatcggcgc
     6301 gaaggcagcg ctgcggacgc ccgggccgcc gccgctggcg ccgaagttga cgtaccaacc
     6361 ggcgtctagc gtcggaatcc agttcagtga gtggggcagg ccggggacgt agccgacgcc
     6421 atagcggcag ttcgttgccg ccaggatatc ctcttgggcc tggggtgtgt cgctctgctg
     6481 ggccgtttcc gcggcgcgta ggccatcgct gctggcttcg atccccgcgg ccagcgcatg
     6541 gaaagccaga acgagcattg ccgacacgac cacaagcccg gcaaacaggt gtatcacgtt
     6601 gctgcgtttc atattgttgt tactacctca tgtttatgta gtctcgcggc ggcccagggg
     6661 atgggc
//
LOCUS       DAOSMQ010000131         6636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11643_length_6636_cov_4.944993, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000131 DAOSMQ010000000
VERSION     DAOSMQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11643_length_6636_cov_4.944993"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PK829_05845"
     CDS             complement(<1..626)
                     /locus_tag="PK829_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40771.1"
                     /translation="MVSLSRPLRSLLAVSVLLLGALLLSQVAQAQGTSQIGRIEITGS
                     NTDSFPSVQLQVYGLDGAGAPIDFATEQLFVSQNNFPVDEVVPDGRVTVGTLTVFLID
                     AAGGITEPLPSIEAAIRQYASAGNMQEQVDYVAVYQNRAAGPQELLAPTPFYNGVSNL
                     FITTPLAAEEGATSLYDAAVRMVELTPGLKPRPEMAAAIVLISDGTDPG"
     gene            692..2563
                     /locus_tag="PK829_05850"
     CDS             692..2563
                     /locus_tag="PK829_05850"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU40772.1"
                     /translation="MIGPRKHISPLFYALALALLAAPSIAAQGVAREALGVRITAVDT
                     ADFPTVRVRVLTTGPGSAPAGDLSRLLLRENGVPIPEATLSSVAVGIDLALVIDANVD
                     FHVADEIGGATRREKVVAAIAGYAGAAMNADGLDRVSVIAPDAARLEPLFLIEDATTA
                     DALTAAVTAFDFAPPADQPLRATPLQAMLTAALDHLAGQTDEGRFQAILLYTDAARID
                     GQLDATALAAAAQMANVPLYVAILGAEAAPDELANAAALTEPTNGQTIHMPTPEAATP
                     LYELFAAQSNQTEMAYVSALRANGTQQVAVNLGNVRDTATFELALAAPEVTLDLPAAI
                     RRVGNSVDTPLALLQPAALSLAAVVRWPDDRPRELTNVTLRVDGEVQSQAAIPQPGPT
                     GRIPLVWDISERDAGDYIVTVEVADALGFQARSAPVTVTIAVARPQPPTPTVAPTPAA
                     PTATLASDAGRLWPLALLLPVALLGLWAIRRARRRAPAPPPPPAASPPRPPARGEFVP
                     LLEWVEGDRREQIELLADNVTLGREPDEVDIVLAGPGVARLHARIRRDDSGQYWLYDE
                     GSATGTFLNHEPLGLAPRALQDGDTVQLGRLTLRFRLEPVALRPAASAEPPPEEKEL"
     gene            2563..3963
                     /locus_tag="PK829_05855"
     CDS             2563..3963
                     /locus_tag="PK829_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40773.1"
                     /translation="MTTNQRPLARRVAAALALLLLSACSVLEPGQLLSPTTPAATGPT
                     APPESTLPGSETPALMCTPPACAPGEVYACASGDCPGGCGTVCAAPTAAPGRPATGPL
                     APAPTDWEGLEIWITDLWRNNVNPAAVRAALQRSGMQHSDADWRAADLNGDLLDEWIA
                     VLYDPSLPGVPFGAAGDLWVINGDGVVFRYYAAPSSDIYSFLAPTLIAVADVTGDHLP
                     EIIADAHFCGAHTCTGNYRVIGQTAAGLADLVQRAPLTDDDPGTTIAMPTADIQVMDT
                     NGDGAADVVVHGGAIGSAGAGTVRTRSEVWRWDGAAVTLAETRLDPTNLRHLLLYEAN
                     DRMAAGDLDGALALYEAVINDPAVVTDSFAYPAEQVTADINRFAAFRLILIDLLQGDS
                     TRAAGRLEWLQTNHPDAPATAAAAALLAGWTGADGQATLCAAIESEMAALDNPTGTLS
                     DMGYGNPSLGAADFCP"
     gene            4047..4310
                     /locus_tag="PK829_05860"
     CDS             4047..4310
                     /locus_tag="PK829_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOU40774.1"
                     /translation="MTLSTVSAKGWVVIPKALRQKYGLEAGSKVRFVDYRGAIYLVPI
                     PDDPIEALYGMFAGGPSMTAELLAERERDNAHEERTLESLRSG"
     gene            4288..4740
                     /locus_tag="PK829_05865"
     CDS             4288..4740
                     /locus_tag="PK829_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOU40775.1"
                     /translation="MNRYVLDSFALLAYIQREPGAIEVKSVLQEAGAGRSAAYMSLIN
                     LGEVLYQLERRQGSDQLPQLLALLDELPLTVVEVTRPRVYAAAHLKAHYAIACGDTFA
                     AALAQELDAVVLTGDPEFRKLGDAVGIQWLPPTVNPSPKEQTPAPPLP"
     gene            complement(4694..5905)
                     /locus_tag="PK829_05870"
     CDS             complement(4694..5905)
                     /locus_tag="PK829_05870"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HOU40776.1"
                     /translation="MDDETTGTTPDGTGNNAERTAQAGAIAGAAAGAAAGAQAGARAA
                     EAAAGGSYEAVWTFRGYKLRPSEFNTAMVHYYRAEIQRSNVWRSRLDTTTNWAVVTVA
                     ASITFVFAAPENHWGVFLLVMLLVLLFLFIEARRYRYYELWSLRARLMETDFFAAMLV
                     PPFAPHADWDETLAESLLQPQFPISRLEAVGRRLRNNYLSIFAILNLGILLKLYLHPF
                     EARSAEMFIERAHLGPFHGGMVLAGLGVFMIVLLLTTLVSQGLQEASGEVLPKYNIED
                     FLGDLWPLGNESDNAAATGVTGTADGSSWSRLSRRKRRRPQLLALVVAANPQHLADRI
                     IAELHRGATALHGQGMYLKQERDVLLVALTVTEIEHLKAIVREEDPQAFITIIPAKEV
                     VGQGFVPLEKD"
     gene            complement(5916..6599)
                     /locus_tag="PK829_05875"
     CDS             complement(5916..6599)
                     /locus_tag="PK829_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40777.1"
                     /translation="MYPEDRVLVAYVPRPADFELIRREGWYRIPQRSAPKGLHAEVIA
                     FYFGRAFEEQKYAIHYYARNAGHELARRRDLLPDEPDHPRADDIYYKVQLGPLQQLAQ
                     PIVSLRWRRLTFIHTTWDRFQEAREINDLFIEGGPYVDRLYATLKERGIPAERDYHVA
                     ETQPEYVVALSVPTRDGRIDVAYGDLPASEGEVAALADALAQAIARQGGVAWLGKNAE
                     DDRPQTPDW"
BASE COUNT         1064 a         2248 c         2253 g         1071 t
ORIGIN      
        1 ccggggtcgg tgccgtcgct gatgaggacg atggccgcgg ccatctccgg ccgcggcttc
       61 aggccgggag tcagctcgac catacgcacc gcggcgtcat agagcgaggt cgccccctct
      121 tcggccgcca gcggtgtggt gatgaacaga ttgctgacgc cgttgtagaa gggcgtgggg
      181 gccaatagct cttgcgggcc ggcggcgcga ttctggtaga cggcgacgta atcgacctgc
      241 tcctgcatgt tgccggcgct ggcgtactgg cgaatggcgg cctcgatgga cggcagcggc
      301 tcggtgatgc caccggcggc gtcgatcaga aagacagtca acgtgccgac cgtcacgcgg
      361 ccgtcgggca caacctcatc gaccgggaag ttattttggc tgacaaagag ctgttcggtg
      421 gcaaagtcga tgggcgcgcc cgcgccgtcc agaccataga cctgcaactg gacgctgggg
      481 aagctatcgg tgttggagcc ggtgatctcg atgcggccaa tctggcttgt cccctgcgcc
      541 tgggcgacct ggctaagaag cagcgcgccc agcagcagga cggacacggc caacaggcta
      601 cgaagcgggc gactcagaga caccatgaca cttctccctt ccattagaag ttatgttgat
      661 ataatagcag acggcgggcg gaaaagaaac aatgatcggc ccacggaaac acatcagccc
      721 acttttttac gccctggcgc tcgccctcct ggccgcgcca agcattgccg cccaaggggt
      781 ggcccgtgag gcgttaggcg tgcgcatcac tgccgtggac accgccgact tccccaccgt
      841 gcgcgtccgc gtgctgacca ccgggccggg cagcgccccg gccggcgacc tctcccgcct
      901 gctgctgcgc gagaatggcg tgcccattcc ggaggcgacg ctgtcgagcg tggccgtcgg
      961 tatcgacctg gccctggtca tcgacgccaa cgtcgatttc cacgtcgccg atgagatcgg
     1021 cggggccacc cggcgcgaaa aggtcgtcgc cgccatcgct ggttatgccg gcgccgccat
     1081 gaacgccgac gggcttgacc gcgtctcggt catcgctccg gacgcggccc ggctggagcc
     1141 gctcttcctg atcgaagacg cgacgacggc cgacgcgctg acggcggctg tgacggcctt
     1201 cgacttcgcg ccgccggccg accaaccgct gcgggcgacg ccgctccagg caatgctgac
     1261 cgcggccctc gaccacctgg ccggccagac ggacgagggg cgcttccagg ccatcctgct
     1321 ctacaccgac gcggcccgca tcgacgggca gttggacgcg acagcgctgg ccgcggcggc
     1381 gcagatggcc aacgtgcccc tctacgtcgc catcctggga gcggaggccg cgccagatga
     1441 gctggccaac gccgccgcgt tgacggagcc aacgaacggc cagaccatcc acatgcccac
     1501 tccggaggcg gcgacgcccc tctatgaact gttcgccgcc cagagcaatc agaccgaaat
     1561 ggcctacgtc tccgccctgc gggccaacgg cacacaacaa gtggccgtca acctgggcaa
     1621 cgttcgcgac acggccacat ttgagctggc cctggccgcc cctgaagtca ccctcgacct
     1681 gcccgccgcc atccgccgcg tcggcaactc tgtggatacg cctctggctc tgctgcaacc
     1741 ggcggcgctg tcgctggcgg ccgtcgtccg ctggcccgac gaccggccgc gcgagctgac
     1801 caacgtgacg ttgcgcgtgg acggcgaagt tcaatcccag gcggccattc cccagcccgg
     1861 ccccaccggg cgcattcccc tggtctggga catcagcgag cgcgacgccg gcgactacat
     1921 cgtgacggtc gaggtagccg atgcgctggg cttccaggcc aggtccgcgc cggtgacggt
     1981 gaccatcgcc gtggcccggc cgcagccgcc gacgccgacg gttgccccga cgccggccgc
     2041 gccgacggcg acgctggcca gtgatgccgg ccgcttgtgg ccgctggccc tgttgctgcc
     2101 cgtggccttg ctgggcctgt gggccatccg ccgggcgcgc cggcgcgccc ccgcgccacc
     2161 cccgccgccg gccgcgtcac cgccgcgccc gccggcgcgc ggcgagttcg tgcctctgct
     2221 ggagtgggtt gagggcgacc gccgcgagca gatcgagctg ctggccgaca atgtgactct
     2281 tggccgcgag ccggacgagg ttgacatcgt tctggccggc cctggcgtgg cccgcctgca
     2341 cgcccgcatc cggcgcgacg acagcggcca gtactggctc tacgacgagg gcagcgccac
     2401 gggcacattc ctaaatcacg agcctctggg tctggctccg cgggcgctgc aagacggcga
     2461 cacggtgcag ttgggccggc tgacgctgcg cttccggctg gagccggtgg cgttgcggcc
     2521 ggcggccagc gcggagccgc ccccggaaga gaaggagttg tgatgacgac gaaccaacgc
     2581 cctttagccc gtcgtgtggc ggccgcgctg gccttgctgc tgctgagcgc ctgttccgtg
     2641 ctggagcccg gccaactgct gtcgccaacc acgcccgcgg caaccggccc gacggctccg
     2701 cccgaatcga cgctgcctgg cagcgagacg ccggcgctga tgtgcacgcc gccggcctgc
     2761 gcccccggcg aggtctacgc ctgtgccagc ggcgactgcc ccggcggctg tggcacggtc
     2821 tgcgccgcgc ccacggccgc gcccggccgc cccgccaccg gcccgctggc ccccgcgccg
     2881 acggactggg aggggctgga gatatggatc acagacctgt ggcgcaacaa cgtcaacccg
     2941 gcggccgtgc gcgccgcttt gcagcggtcg ggcatgcagc acagtgacgc cgactggcgc
     3001 gccgccgatc tgaacggcga cctgctggac gagtggattg ccgtgctcta tgacccgtcg
     3061 ctaccgggcg tgcccttcgg cgcggccggc gatctgtggg tcatcaacgg cgacggcgtc
     3121 gtcttccgct actacgccgc gcccagcagc gacatctata gcttcctcgc gccgacgctg
     3181 attgccgtgg ccgacgtgac cggcgaccat ctgccggaga tcatcgccga cgcccacttt
     3241 tgcggcgcgc acacctgcac cggcaactac cgcgtcatcg gccagacggc cgcgggcctg
     3301 gccgacctgg tgcagcgcgc gccgctgact gacgacgacc ccggtacgac catcgccatg
     3361 cccacggccg acattcaggt gatggatacg aacggcgacg gcgcggctga tgtcgtcgtc
     3421 cacggcgggg ctatcggctc ggccggggcg ggcacggtgc gaacgcgcag cgaggtctgg
     3481 cgctgggacg gcgcggcggt gacgctggcc gagacgcgcc tcgacccgac caacttgcgc
     3541 cacttgctgc tctacgaggc caatgaccgg atggccgccg gcgatcttga cggggcgctg
     3601 gccctctatg aggcggtcat caacgatccg gccgtcgtca ccgacagctt tgcctacccg
     3661 gccgagcagg tcacggccga catcaaccgc ttcgccgcct tccggctcat cctgatcgac
     3721 ctcttacagg gagattcaac gcgcgcggcc ggccggctgg agtggttgca aaccaaccac
     3781 cccgacgccc cggcgacggc cgccgcggcg gcgctgctgg ccggctggac gggcgcggac
     3841 gggcaggcca ccctctgcgc cgccatcgag agcgagatgg ccgcgctgga caacccgacg
     3901 gggacgctga gcgatatggg ctacggcaat cctagcctgg gcgcggccga tttttgcccg
     3961 taggtatgac agttcattga attcatactc atgttaggcc cgttgtgtat actaatggag
     4021 tatttgtata tacaagggag acttctatga cactatcaac tgtttcggcc aagggctggg
     4081 tcgtcattcc caaagcgttg cgccaaaagt acggtctgga ggccggctcc aaggtgcgtt
     4141 tcgttgacta ccgaggagct atctacctgg tcccaattcc tgacgaccct attgaggcct
     4201 tgtacgggat gtttgccgga gggccgtcaa tgacagccga actcctggcc gagcgcgaac
     4261 gcgacaacgc acacgaagag aggacgcttg aatcgctacg ttctggatag ttttgctttg
     4321 ttggcctaca ttcagcgtga accaggggca atcgaggtta aatcagtcct gcaagaggct
     4381 ggggccggcc ggtcggcagc atatatgagt ctgatcaatc tgggcgaagt cctctatcag
     4441 cttgagcggc gtcaaggcag cgaccaactg ccgcagttgc tggcgctact ggatgagttg
     4501 ccgctcacgg tggtcgaagt gacgcgaccg cgggtctacg ccgcagccca tcttaaggct
     4561 cactacgcta tcgcttgcgg cgacaccttc gccgctgcgc tggcccaaga attggatgct
     4621 gttgtgctga ccggcgatcc tgagttccgc aagctgggtg acgcagtggg cattcagtgg
     4681 ttgccaccaa ccgttaatcc ttctccaaag gaacaaaccc ctgccccacc acttccttag
     4741 ccgggatgat ggtgatgaag gcctggggat cttcctcgcg aacgatggcc ttcaggtgct
     4801 cgatctcggt cacggtcagg gccaccagca gcacgtcacg ctcctgcttc aggtacatgc
     4861 cctggccgtg cagcgccgtc gccccacgat gcagttcggc gatgatacgg tcggccaggt
     4921 gctgcgggtt ggcggccacg accagggcca gcaactgtgg gcggcggcgc ttgcggcggc
     4981 tgaggcgcga ccaggacgaa ccatcggccg tgccggtcac gccggttgcg gcggcgttgt
     5041 cgctctcgtt gcccagcggc cagaggtcgc ccaggaagtc ctcgatgttg tacttgggca
     5101 gcacctcgcc gctggcctct tgcagcccct gcgatacgag cgtggtgagc aacagcacga
     5161 tcatgaagac gcccagcccg gccagcacca tgccgccgtg gaacggcccc aggtgggcgc
     5221 gctcgatgaa catctccgcc gagcgtgcct cgaaggggtg gaggtagagc tttagcagga
     5281 tgcccaggtt caggatggcg aagatagaca ggtagttgtt gcgcaggcgg cggccgacgg
     5341 cctccagccg cgagataggg aattgcggct gcaagagaga ctcggccagc gtctcgtccc
     5401 aatcggcgtg gggcgcgaag ggaggcacga gcatggcggc gaagaagtcg gtttccatca
     5461 gccgggcgcg caggctccac aactcgtagt agcggtagcg ccgcgcctcg atgaagagga
     5521 acagcagcac cagcagcatc accagcagaa agacgcccca gtggttttcc ggcgcggcga
     5581 aaacgaaggt gatcgacgcg gccacggtga cgacggccca gttggttgtc gtgtccagcc
     5641 ggctgcgcca gacgttggag cgctgaatct cggcgcggta gtagtgcacc atggctgtgt
     5701 tgaattccga cggccgcagc ttgtagccgc ggaaggtcca taccgcctca tagctgccgc
     5761 cggcggccgc ttcggcggcg cgggcgccgg cctgcgcgcc cgcggcggct ccggcggcgg
     5821 ctccggcgat cgcgcccgcc tgcgcggtgc gctccgcgtt gttgccggtc ccgtccggcg
     5881 tcgtgcccgt tgtctcatcg tccatagccc gcacctcacc aatccggcgt ctgtggccgg
     5941 tcgtcttcgg cgttcttgcc cagccaggcg acgccgccct gacgggcgat ggcctgcgcc
     6001 agagcgtcgg ccagggccgc cacctcgccc tcgctggccg gcaggtcgcc ataggccacg
     6061 tcgatgcggc cgtcgcgcgt gggcaccgac agggccacga cgtactccgg ctgcgtttcg
     6121 gccacgtggt agtcgcgctc ggcggggatg ccgcgctctt tcagcgtcgc atagaggcgg
     6181 tcgacgtatg gcccgccctc gatgaacagg tcgttgatct ccctggcctc ctggaagcgg
     6241 tcccaggtgg tgtggatgaa ggtcagccgc cgccagcgca ggctgacgat gggctgggcc
     6301 agttgttgca gcgggccgag ttggactttg tagtagatgt catcggcgcg ggggtggtcg
     6361 ggttcgtcgg gcagcaggtc gcggcggcgg gccagctcgt ggccggcgtt gcgagcgtag
     6421 tagtgaatgg cgtacttttg ctcctcgaag gcgcggccga agtagaaagc gatgacctcg
     6481 gcgtgtaagc ccttgggggc gctgcgctgg gggatgcgat accagccctc gcggcgaatc
     6541 agctcgaaat cggccggccg gggcacatag gccaccagga cgcgatcttc cggatacatg
     6601 cgcgcaagtg tacctgtaga gaagaagtta acgcaa
//
LOCUS       DAOSMQ010000132         6550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11854_length_6550_cov_4.498383, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000132 DAOSMQ010000000
VERSION     DAOSMQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11854_length_6550_cov_4.498383"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="PK829_05880"
     CDS             <1..1647
                     /locus_tag="PK829_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU40778.1"
                     /translation="AYFEVLHHPLEDIGVDFWWMDWQQGTETAIGGLDPLWWLNHLHF
                     HDLGRHGKRPFVFSRWGGLGNHRYPIGFSGDTVVSWASLAFQPYMTATAANVAYGWWS
                     HDIGGHMRGVEDGELFTRWVQFGVFSPIFRLHSTKNPFHERRPWGYDAEVLRVTREAM
                     QLRHALIPYLYTLSRRDETEAVAPVRPMYHDYPNHDAAYACPNQYLFGDLVVAPFLSP
                     AEADTRLARAVVWLPPGDWYHFFTGQHYRGDAWYALYGALEDIPVFARAGAIVPLGPR
                     VGWGGVECPQELHMHVFAGADGRFSLYEDDGETTAYRDGAFVQSGFEQRWDGQQLRLT
                     ISPAAGERTLLPAGRRVYIHIHGLAAPAAVTLLTDGQTQAVAFSYDAAEEALHLAPLQ
                     LDGAGAQLTVRAALSRRDRTAETCLRLLGSFRLDSGVKEFLAQRLARLPGRPELLADF
                     GVDLSAAQARALLEVSQGVGVQMTRDDDGLLLALWNNAERPGFAYHFAEIRPLKWFIP
                     ERYNATSGQVPRQTFIRPGRSDWRLRADYFGLAGAHIGSD"
     gene            1954..3321
                     /locus_tag="PK829_05885"
     CDS             1954..3321
                     /locus_tag="PK829_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU40779.1"
                     /translation="MIVVLTAFVTIGLMLLLTALYVGAEFATVAARRTRIGQMAAQGD
                     SLARMLLPYIEDSRKLDRYVAACQVGITISSLVLGAYAQGAIAMRLAGPLGNLLGNVV
                     SPTTATRLGQSIAVIGVLFVITALQVILGELLPKSLAIQNPEGMARAVVWPMRFSLLL
                     FAVPIWLFNGSANLLLRLFGRDTHQASGRAHSAEEIELLVTESYEGGLLDDNERRLLR
                     NALRMRELTARQVMIHRTRITAAPIETNALELMQLAVEAGYSRIPLYRETIDDIVGFV
                     HVKDVFRVYHRGERDITTAVREVVFVPETLAAGEVWDRLEEGHGYLAIVFDEFGGTAG
                     LLTLEDLIEEIFGELQDEFDDEMAAIARDKEGRIYLRGDLLVSDVNEYLELELPEEGA
                     DTLSGLVFSQLGRPPLAGDEVRVAQVVIRVETMEDLGVGEVSLTLPPREADSPFSEWD
                     IAEHD"
     gene            3314..4768
                     /locus_tag="PK829_05890"
     CDS             3314..4768
                     /locus_tag="PK829_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU40780.1"
                     /translation="MTKLALLLLGLPGVSFFVAAAAAGESLPSFTVLIVPAIVIFVLV
                     IINGVFVAAEFAIIGVRDTQMEQMADEGNSVAGNVLQVLELRPRLDHYIATAQLGITL
                     ASLGLAMYGEPAISHFIEPYLERFGGLTPATTATIGYIVALSLMTYLHVVVGEMVPKA
                     LALSDASSMAIRLDPLMRFTGGVLKYPVHLLNAIGNRLLRLLRIPPAHGEERLVAADE
                     LELIVAESAEGGLIKDEEEEFIRNIFDFSERTVGQVMTPRRRMQALDARWPQDELIRI
                     VTESRHSRFPVYENDLDHIVGILHLKDLLRLTLRPNGPFDLRLILRAAPEVPEDYRVD
                     RLLAAFKKQKLHMAVVRDEFGGLAGVVTLEDLVEEVVGEVRDEFDQEREPRVELGPGV
                     LDVAGDYLLDDLAEEVFLGEADDLPDVETVGGLIVALLGRPPLMGDTVALREVHFTVL
                     DIDRLAVLRAKVEFPSHKTHKAGGRESETDKEEE"
     gene            4823..5212
                     /locus_tag="PK829_05895"
     CDS             4823..5212
                     /locus_tag="PK829_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40781.1"
                     /translation="MGTLMTIHGEVRWLVAILAVVIIIKFAIGWLGRRGYTSLDRGLL
                     SAYTILLDINVLLGLVLLFFNGGINGPRLEHATTMLLAVIAGHMTAMWRRSTDSATKY
                     RNQLLLVLLSLLLVFFGVIRLRGGFTF"
     gene            complement(5309..>6550)
                     /locus_tag="PK829_05900"
     CDS             complement(5309..>6550)
                     /locus_tag="PK829_05900"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOU40782.1"
                     /translation="RNYADERKLPADYVARAAEVSGRAREAWVKARAEDDFAHFRPWL
                     EQVVSLCQEMAGHYGYEDEPYDALLDKYETHMKTAEVRTIFDALKAELVPLREAIDQS
                     GAQLDDSILHQPYDVARQQDFARIIAAALGYDFDRGHLGTVVHPFATSFSRNDARITT
                     RWYPDFLSPSLFGTLHECGHAIYEQGTAPELARTPLARGTSMGIHESQSRMMENLVGR
                     SRPFWQAHFPTLQAHFPEALGHSTAEDFYRAINKTQPSYIRVEADELTYNFHIILRFE
                     LEQAMLNGDLAVADLPAAWADKMQTLLGITPPDDRQGCLQDVHWSRPGFGYFPTYALG
                     NLYAAQFFETAAAQDSSVTEELDRGQTGALLNWLRQNIHRHGKKFVPGELVQRVTGRP
                     LSHDAFMRYATAKFSDLYGLN"
BASE COUNT         1021 a         2112 c         2200 g         1217 t
ORIGIN      
        1 gcctacttcg aggtactcca tcacccgctg gaggacatcg gcgtcgattt ctggtggatg
       61 gactggcagc agggcacgga gacggccatt ggcgggttag acccgctgtg gtggctcaac
      121 catctgcatt tccacgatct gggccgccac ggcaagcggc cgtttgtctt ctcgcgctgg
      181 ggcgggctgg gcaaccaccg ttaccctatc ggtttctccg gcgacacggt cgtctcctgg
      241 gcgtcgctgg ctttccagcc ctacatgaca gccacggcgg ccaacgtcgc ctatggttgg
      301 tggagccacg acattggtgg ccacatgcgc ggcgtcgagg acggcgagct attcactcgc
      361 tgggtgcagt tcggcgtctt cagccccatc ttccggctgc acagcaccaa gaacccattc
      421 cacgagcgac ggccgtgggg ctacgacgcc gaggtgttgc gcgtcacccg cgaggccatg
      481 cagctgcgcc acgcgctcat tccctacctc tataccctct cgcggcggga cgaaaccgag
      541 gcggtcgcgc cggtgcggcc gatgtaccac gactacccca accacgatgc tgcctacgcc
      601 tgccccaacc agtacctatt cggtgatctc gtcgtcgctc cgttcctgtc gccggccgag
      661 gccgacacac gcctggcgcg cgcggtcgtc tggctgccgc cgggcgactg gtatcacttt
      721 ttcaccgggc agcactatcg cggcgatgcc tggtacgcgc tctacggcgc gttggaggac
      781 atccctgtct ttgcccgcgc cggggccatc gtgcccctgg ggccgcgtgt cggctggggc
      841 ggcgtagagt gtccgcagga attgcacatg cacgtttttg ccggggccga tggccgcttc
      901 agcctttatg aagacgacgg cgagacgacg gcctaccggg acggcgcatt tgtgcagtcg
      961 ggttttgaac aacgttggga cggccagcaa ttgcggctga cgatcagccc ggcggccggc
     1021 gagcgaacgc tgctgcccgc cggccgccgc gtctacatcc acatccacgg cctggccgcg
     1081 cccgcggcgg tgacgctgtt gactgacggg cagacgcagg cagtggcgtt tagctatgac
     1141 gcggcggaag aagcgttgca cctggccccg ctgcaactgg acggcgcggg cgcgcaactg
     1201 acggtcaggg ccgccctgtc ccggcgcgac cgcaccgcag agacgtgtct gcgcctgcta
     1261 gggtcgttcc ggctcgattc cggcgtcaag gagtttctgg cccagcgact ggcgcggctg
     1321 ccgggccggc cggagttgct ggccgacttc ggcgtcgatc tgtccgcggc gcaggcgcgg
     1381 gcgctgctgg aagtgagcca gggcgtcggc gtccagatga cccgcgacga cgatggcctg
     1441 ctgctggcgc tatggaacaa cgcggagcga ccaggcttcg cctaccactt cgccgagatc
     1501 cggccgctga agtggttcat cccggagcgc tataacgcca cctccggcca ggttccgcgc
     1561 cagacgttca tccggccggg gcgctcggac tggcgattgc gggcggacta ctttggtctg
     1621 gccggcgctc acattgggag cgattgactt tcgccccgtg gtcactataa tcgctttgtc
     1681 aacgaactgg cgagagtctc cttgcccacc gtctgacacc caacgatcga tagatggata
     1741 ccagcgaatt gaagagcctg gccgcgcacc ggcgcggtcg ccagggagaa tgtcactctt
     1801 gagcgacccc gattcggagc cgccgaacct ttgcccccac gactccaccg acaacaccgc
     1861 atcggctgcg gggcatggcc gacccaccgg gccgcgctct ggccgcggca ttgttgcccg
     1921 cgggcgggcc acccgcgccg gcactgagat gccatgatcg tcgtcctcac cgctttcgtc
     1981 accattggcc tgatgctgct gctgacggcg ctctacgtcg gcgcggagtt cgccacggtg
     2041 gccgcgcgcc gcacgcgcat tggccagatg gccgcccagg gggactcgct ggcgcggatg
     2101 ctgctgccct acatcgagga cagccgcaag ctcgaccgct acgtggccgc ctgtcaggtg
     2161 ggcatcacca tctcatcgct ggttctgggc gcgtatgccc agggggccat cgccatgcgc
     2221 ctggccgggc cgctgggcaa cctgctgggc aacgtcgtct cgccaacgac ggcgacgcgg
     2281 ctgggccagt cgatcgcggt catcggtgta ctgttcgtca tcaccgcttt gcaggtcatc
     2341 ctgggcgagt tgctgcccaa gtcgctggcc atccagaacc cggaggggat ggcccgcgcc
     2401 gtcgtctggc ccatgcgctt ctccctgctg ctcttcgccg tgcccatctg gctgttcaac
     2461 ggcagcgcca acctgctgct gcgcctgttc ggccgggaca cgcatcaggc atccggccgg
     2521 gcgcactcgg ctgaagagat cgaactgctg gtgacggaaa gctacgaggg cgggctgctg
     2581 gatgacaacg agcgccgcct gctgcgcaac gccctgcgca tgcgcgagtt gaccgcccgc
     2641 caggtgatga tccatcgcac gcgcatcacc gccgcgccca ttgaaaccaa cgcgctggag
     2701 ctgatgcagt tggccgtcga ggcaggctac tcccgcattc ccctctaccg cgagacgatt
     2761 gacgacatcg ttggcttcgt ccacgtcaag gacgtgttcc gcgtctatca ccgcggcgaa
     2821 cgggacatca ccacggctgt gcgcgaggtc gtcttcgtgc cggagacgtt ggccgcgggc
     2881 gaggtttggg accggctgga ggagggccac ggctatctgg ctatcgtctt cgatgagttc
     2941 ggcggcacgg ccgggttgct gaccctggag gacctcattg aggagatctt cggcgagctg
     3001 caagatgagt tcgacgatga aatggccgcc attgctcgcg acaaggaagg ccgcatctat
     3061 ttgcgcggcg acctgctggt gagcgatgtc aacgagtatc tggagttgga actgccggag
     3121 gagggcgcgg acacgctcag cggacttgtc ttcagccaac tgggccggcc gccgctggcc
     3181 ggcgacgaag tgcgcgtcgc tcaggtcgtc atccgcgtgg aaacgatgga agacctgggc
     3241 gtgggcgagg tgtcgctgac gctaccaccg cgcgaggccg actcgccctt ttcggaatgg
     3301 gatatcgccg agcatgacta aattggccct cctcctgctg ggcttgcccg gtgtaagctt
     3361 tttcgtggcc gcggccgccg cgggcgaatc gctgccgtcg tttacggttc tgattgtgcc
     3421 ggccatcgtc atttttgtgc tggtcatcat caatggcgtt ttcgtggccg ccgaattcgc
     3481 catcatcggc gtgcgcgata cccagatgga gcagatggcc gacgagggca acagcgtcgc
     3541 cggcaacgtc ttgcaggtgc tggaactgcg tccgcgcctc gaccactaca tcgctacggc
     3601 tcaactgggc attactctgg cctcgctggg gctggccatg tatggcgagc cggccatcag
     3661 ccacttcatc gagccgtacc tggagagatt cggtggcctc accccggcga cgacggcgac
     3721 catcggctac atcgttgccc tgtcgctgat gacctacctc cacgtcgtcg tcggcgagat
     3781 ggtgcccaag gcgctggcgc tatccgacgc ctcctcgatg gccattcgtc tcgatccgct
     3841 gatgcgtttc accggcggcg tgctcaagta cccagtgcat ctgctcaacg ccattggcaa
     3901 ccggctcctg cgcctgttgc gcattccgcc ggcccacggc gaagagcggc tggtggccgc
     3961 cgacgagctg gaattgatcg ttgccgagag cgccgagggc gggctgatca aggacgagga
     4021 agaggaattc atccgcaaca tcttcgactt cagcgagcgc accgttggcc aggtgatgac
     4081 gccgcgccgg cgcatgcagg ccctcgacgc gcgctggcca caggatgagt tgatccgcat
     4141 cgtcacggag agtcgtcaca gtcgcttccc cgtttacgag aacgacctcg accacattgt
     4201 cggcattctg cacctgaagg acctgctgcg gctaaccctg cggcctaacg ggccgttcga
     4261 cctgcgcctc atcctgcggg ccgcgccgga agtgccggag gactaccgcg tcgaccggct
     4321 gctggccgcc ttcaagaagc agaagctgca catggccgtg gtgcgcgacg agttcggcgg
     4381 gctggccggc gtggtgacgt tggaagacct ggtggaggag gtcgtcggtg aggtgcgcga
     4441 cgagttcgat caggagcgcg agccgcgcgt ggagcttggc cccggcgtgc tagacgtagc
     4501 tggcgactac ctgctcgacg acctagccga ggaagtgttc ctgggcgagg ccgatgacct
     4561 gcccgacgtg gagaccgtcg gcgggctgat cgttgccctg ttgggccggc cgccgctgat
     4621 gggcgacacg gtggctctgc gtgaagttca ttttaccgtt ctcgacattg accggctggc
     4681 cgttctgcgg gccaaggtgg agtttcccag ccacaagacc cacaaggccg gcgggcgcga
     4741 gtcggagaca gacaaagaag aagagtagag accacgaccg cccggtcgcg gctcgctcgc
     4801 caacaaagga gtcttacatc ttatgggtac actcatgacc atccacggcg aagtacgctg
     4861 gcttgttgcc attctggccg tggtcatcat catcaagttc gccatcggct ggttgggccg
     4921 caggggctac acctcgctcg atcgcggcct cctgtcggcc tataccatcc tgctcgacat
     4981 caacgttctg ctggggctgg tgctattgtt tttcaacggc ggcatcaacg ggccgcggct
     5041 ggagcacgcc acgacgatgc tgctggcggt catcgccggg cacatgacgg ccatgtggcg
     5101 gcgttcgacc gactcggcga ccaagtaccg caatcaactc ttgctggttc tgttgtcgct
     5161 gctgctggtc ttctttggcg tcattcgtct gcggggcggc ttcacgttct agcaccgccg
     5221 cgccgccgac aaacgcgccg gccgccgggc gtggggagta tctcccgcgt ccgacggccg
     5281 gcgttttcgt ttgtgggtaa tgtgtcgctc agttcaggcc atagaggtca ctgaacttgg
     5341 ccgtggcgta gcgcatgaag gcgtcgtggc tcagcggccg gccggtgacg cgctgcacca
     5401 gctcgccggg gacgaacttc ttgccgtggc ggtggatgtt ctggcgcagc cagttcagca
     5461 gcgcgcccgt ctggccgcgg tctagctcct cggtgacgct cgagtcctgc gccgcggccg
     5521 tctcgaagaa ctgcgccgcg tagaggttgc ccagggcgta ggtggggaag tagccgaagc
     5581 ccggccgcga ccagtggaca tcttgcaagc agccctggcg gtcgtcgggc ggggtgatgc
     5641 ccagcagggt ctgcatcttg tcggcccagg ccgctggcag gtcggccaca gccagatcgc
     5701 cgttgagcat ggcctgctcc agttcaaagc gcaggatgat gtggaagttg tacgtcagtt
     5761 cgtccgcttc gacgcgaata taagagggct gggtcttgtt gatggcgcgg tagaagtcct
     5821 cagccgtgct gtggcctagc gcctccggga agtgggcttg cagggtggga aagtgggcct
     5881 gccagaaagg gcggctgcgg ccgacgaggt tctccatcat gcgcgactgc gactcgtgga
     5941 tgcccatcga cgtgccgcgg gccaacggcg tgcgcgccag ttccggggcc gtgccctgct
     6001 catagatggc gtggccgcac tcgtgcagcg tgccgaagag cgacggcgac aggaagtcgg
     6061 gataccagcg cgtggtgatg cgggcgtcgt tgcggctgaa gctggtggcg aaggggtgga
     6121 cgactgtgcc caggtggccg cggtcaaagt cataacccag ggcggcggcg attatgcggg
     6181 cgaagtcctg ctgcctggcc acgtcgtagg gttggtggag gatgctgtca tccagttgcg
     6241 cgccgctctg gtcaatggcc tcgcgcaggg gcacaagctc agccttcagc gcgtcgaaga
     6301 tcgttcgcac ctcggccgtc ttcatgtgcg tctcatactt gtccagcagg gcgtcgtagg
     6361 gttcgtcctc atacccatag tggccggcca tctcctgaca caggctgacg acctgctcca
     6421 accacggccg gaagtgggcg aaatcgtctt cggcgcgggc cttcacccag gcctcgcgtg
     6481 cccggccgct gacctcggcc gcgcgggcca cgtagtcggc cggcagcttg cgctcgtcgg
     6541 cgtagttgcg
//
LOCUS       DAOSMQ010000133         6518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11919_length_6518_cov_4.717004, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000133 DAOSMQ010000000
VERSION     DAOSMQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11919_length_6518_cov_4.717004"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PK829_05905"
     CDS             <1..565
                     /locus_tag="PK829_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU40783.1"
                     /translation="WAVRGTDLCHTHHRNPPLPPPPGWDDLSLLEEEVRRLLEARAEF
                     QAWAREQRAAGGTGELSPLQFLKAWGDASARLVQLIRARREMAPREDALEELAEAVWR
                     DLLARHGGDDRVALVIHGGFFRALFNVLVDAPHDRPSPLGLKGWWIAMNNASITRIDF
                     DEEAGERWAAIVYVNKVEHLTDDLLSY"
     gene            895..1887
                     /locus_tag="PK829_05910"
     CDS             895..1887
                     /locus_tag="PK829_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU40784.1"
                     /translation="MMHKGEIVVENLVKHYPGDVVAVDGVAMQVEAGRIFGFLGPNGA
                     GKSTTIKILTTLALPTSGRATVGGYDVVTQAEAVRRIAGVALQDIGLDPLMKPLELLR
                     LQTRMFGATRDQAEARSRELLELVHLTEAIDRRVGTYSGGMRRRLDLALALAHEPQVL
                     FLDEPTTGLDPASRRDVWDEVRRLNRELGMTIFLTTQYLEEADELADVVAIIDKGRIA
                     VQGSPARLKSSVGNESINLAFEDRNTVERALSHLEGFGQRAQIDRDVLRLYMENAAEA
                     IPAVVNRLREANVSPISLTLTQPTLDDVFLQVTGQRLRDEAQAPPALATARHGR"
     gene            1904..2728
                     /locus_tag="PK829_05915"
     CDS             1904..2728
                     /locus_tag="PK829_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014970174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40785.1"
                     /translation="MAPLTVPTVHHNRFEKRAGLLSQIGMVTRRNLTVILRTPQALLP
                     PLIISVFFLVIYESTLGEAAGFLPGLSGNSYLGFILPLSLVSSALAGSGLAAQNLVRD
                     IESGYFDKLLLTPASRAALLLGPILAGGVILGLQALFVIGVGLLMGLEPATGVAGLLV
                     IIGLAILLGTGFAGFTVSAALGSGSAAVTQAASFLFFPLTFLTASFVPLELLSGWLRA
                     AARVNPITYVLEAMRALLNDGWNMGLLWQGVLACLALAVAMYALAVYALRLRTRRS"
     gene            complement(2783..4597)
                     /locus_tag="PK829_05920"
     CDS             complement(2783..4597)
                     /locus_tag="PK829_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOU40786.1"
                     /translation="MEPRLHALVLYKAQPAVVRATTDKKLTIELPNGERVNVRPKDVA
                     VLHPGPAADLRRLPPPEGDPLTAWELLDSGHTTLPELAELAYGDYTPAGAWAAWQLVA
                     DGLYFGGAPDAIIVYPASKVAETLAAREARAAEERAWAAFVARAAAGQTAPEDAPYLA
                     DVVALALDQTESSRTLRRLDRPQTREAAHAFLLAAGVWTPADNPYPARLNLAVSRVDV
                     PIGPLPDEPRRDLTHLLALAIDDEGSSDPDDAVGLADGVLWVHIADAAALVPPDSPAD
                     REARARAANLYLPEGTARMLPDELTDRLALGLQEVSPALSLAFAAGGDAPELLEIVPS
                     WVRVTRTTYEAAEACLGESPYRELLALAGAARARRVANGAVEIDLPEVKVRVRDDGQV
                     AITPLPPLRSRELVREAMLTAGEAIGRLAAARGLPLVFTHQEPPQEVESPAGGLAGMF
                     AQRRAMQRSQPRVAPGRHAGLGLDVYVQATSPLRRYGDLLNHQQLRAHLRGEPPLDAG
                     EMTLRLGMADAVAGAVRTAERLSNAHWAMVYLLQNPHWTGEGVVVEQKPGREVVLIPA
                     LAIETELYGRKPRPLNSMVRLAVESVDLPQRTARFRAE"
     gene            complement(4597..5415)
                     /locus_tag="PK829_05925"
     CDS             complement(4597..5415)
                     /locus_tag="PK829_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU40787.1"
                     /translation="MTSFAGKNVLITGSASGIGRLMAQKIAARGGHVILWDIDRAGLE
                     ALADQLEHWGYRAATYQQCDLSDREEIETTARLVLAEHGDVDILINNAGIVSGKPLLE
                     LTDEAIKRTFDINTLALFRTTRCFLPGMIRQGSGHIVTVASAGGLIGVPRMTDYSASK
                     FAAIGFDESLRFELKRLGHRGIRTTVVCPYYINTGMFRGVKTRFPFLLPIMEPDDVAS
                     RIVAAIERNKQRLVLPPFVLALFPTRLLPPALFDPVISFFGINHTMDDFKGRGG"
     gene            5606..6418
                     /locus_tag="PK829_05930"
     CDS             5606..6418
                     /locus_tag="PK829_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOU40788.1"
                     /translation="MNTNSYDISSDYHMHSTFSMDGQDTIEALGWRALALGFNEIAIT
                     EHAEWHPAWRGSFDVDGYFAEIARCRALFAPQGLTLYSGVEMGNPHEFAREASALTAA
                     YPFDVRIASLHWLHDHNIHDSACFAGHDPLDVYADYFVALGHMAADFSDIDVIAHFDR
                     ILWRGSLLGATFAPRAIENLVRDALATIAWRGLALELNTRNLNHMPNWRPALTTMLRW
                     FGEEGGRRVVVNSDAHRVGQMGANLELAAAVLREAGLAATTLQPALVAAVRG"
BASE COUNT         1031 a         2243 c         2172 g         1072 t
ORIGIN      
        1 ctgggccgtg cgcggcaccg acctgtgcca cacgcaccac cgcaatccgc ccctgccccc
       61 gccccccggc tgggacgacc tctcgctgct cgaggaggag gtgcgccgcc tgctcgaggc
      121 gcgcgccgag ttccaggcct gggcccgcga gcagcgcgcg gccggcggga cgggcgagct
      181 cagcccgctg cagttcctca aagcctgggg cgacgcctcg gcccgcctgg tgcagctcat
      241 ccgcgcgcgc cgcgagatgg ccccgcgcga ggacgccctc gaggagctgg ccgaagccgt
      301 ctggcgcgac ctgctggccc gccacggcgg cgatgaccgc gttgccctgg tcatccacgg
      361 agggttcttc cgcgcgctct tcaatgtatt ggtcgatgcc ccccacgacc gcccctcccc
      421 cctcggcctc aagggctggt ggatcgccat gaacaacgcc tccatcaccc gtatcgattt
      481 tgacgaggag gcgggcgagc gctgggcagc tatcgtctac gtcaacaagg tcgagcattt
      541 gaccgacgac ctgctgagct actaaagccc cctccacggg ccaacagacc aacagaccga
      601 ccacggcccc gccaccggtt tccggcggcg accgcctgtt tttgtttggc ccaacgggtt
      661 tagcagggct ttgccaaagt caccccaggc gcaccgacct gacaggtagg cggccaggca
      721 cgagactgcc cccagagaac gactggccgc tcacctatca ggtcaatggt cgccagacga
      781 ctttggcaaa gccccgacga atttagacaa aaccttgaca tattctgtac ttatgctaga
      841 ctactcctgg ttggggcgag ggcgcgagcc ggcagaggcc ccaccgagga gagcatgatg
      901 cacaaaggcg aaattgtcgt cgagaatctc gtcaagcact accccggcga tgtcgtcgcc
      961 gtggatggcg tagcgatgca agtagaagca ggccgtatct tcggctttct ggggccgaac
     1021 ggcgcgggca agagcacgac gatcaaaatc ctgacaacgt tggccctgcc caccagcggc
     1081 cgggccaccg tgggcggcta cgatgtcgtg acgcaggcgg aagctgtgcg ccgcattgcc
     1141 ggcgtcgcgc tgcaagacat cggcctcgat ccgctcatga agccgctgga actgttgcgc
     1201 ttacagacgc gcatgttcgg ggccactcgc gaccaggccg aggcgcgcag ccgggaacta
     1261 ctcgaactcg tccacctgac cgaggccatc gaccggcgtg ttggcaccta cagcggtggc
     1321 atgcgccgcc gtctcgacct ggccctggcc ctggcccacg aaccacaggt cttgttcctc
     1381 gacgagccga cgaccggcct tgacccggcc agccgccgcg acgtatggga cgaggtacgc
     1441 cgcctgaacc gcgagttggg catgaccatc ttcctgacca cccaatacct ggaagaggcc
     1501 gacgaactgg ccgacgtcgt ggccatcatc gacaagggcc gcatcgccgt gcaaggctcc
     1561 cccgcccggc tgaagagcag cgtcggcaac gagtcgatca acctggcctt cgaggatcgc
     1621 aatacggtgg aacgagcttt gtcccacctg gaggggtttg gccagcgagc acagatcgac
     1681 cgcgacgtgc tgcgcctcta catggaaaac gccgccgagg ccattccggc cgtcgtcaac
     1741 cggctgcgcg aagccaacgt cagtcccatc tcgctgacgc tcacccagcc cacgctggac
     1801 gacgtttttc tgcaagtgac tggccaacga ctgcgcgacg aggcccaggc cccgcccgct
     1861 ttggccacgg cacgccacgg ccgctaatca aggagtcgat ctcatggcac cattaaccgt
     1921 ccccactgtc caccacaacc gcttcgaaaa gcgcgccggc cttctgtcgc agattggcat
     1981 ggtcacccgt cgcaatctga cggtcatcct gcgcacgcca caggcgctgt taccgccgct
     2041 gatcatcagc gtcttcttcc tggtcatcta cgagtccacg ctgggcgaag ctgccggctt
     2101 tctgcctggc ctgagcggca acagctatct gggcttcatc ctgcccctgt cgctggttag
     2161 ctccgccctg gccggctccg gtctggccgc gcaaaacctc gtgcgcgata ttgagtcggg
     2221 ctacttcgac aagctgctgc tgacgccggc cagccgcgca gccctcttac tcggcccgat
     2281 tctggccggc ggcgtcatcc tcggcctgca agccctgttc gtcatcggcg tgggtctgct
     2341 gatgggtctg gagccagcga cgggagtggc cggattgctg gtcatcatcg ggctggccat
     2401 tctgctgggc acaggcttcg ccggattcac ggtctcggcc gcgctcggtt ccggcagcgc
     2461 tgctgtcact caggcagcca gtttcctctt cttcccgctg acgttcctga cggccagctt
     2521 cgtgccgttg gaactactca gcggttggct ccgggcggca gcgcgcgtca atcccatcac
     2581 ctacgtcctc gaggcgatgc gtgccctgct caacgacggc tggaacatgg gcctgctgtg
     2641 gcagggcgtc ctggcctgcc tggccctggc cgtggccatg tacgcgctgg cggtgtacgc
     2701 gctgcgtctg cggacgcgcc gcagttagcg gctgatgaca gacgacggcc gcggtcagtt
     2761 ccgcggcccg tcgtcccggc ccctactcag cccggaagcg cgccgttcgc tgcggcaggt
     2821 caaccgactc aacggccagc cgcaccatgc tgttgagcgg ccgcggcttg cggccatata
     2881 gctccgtttc gatggccagc gcggggatga gcaccacctc gcggccgggc ttttgctcca
     2941 cgacgacgcc ctcgcccgtc cagtgcgggt tctgcaacag gtagaccatt gcccagtggg
     3001 cgttcgacag ccgctcggcc gtgcgcaccg cgccggcgac ggcatcggcc atgcccagtc
     3061 gcagcgtcat ctcgcccgcg tccaatggcg gctcgccgcg caagtgggcg cgcagttgct
     3121 ggtggttcag caggtcgcca tagcgccgca gcgggctggt cgcctgcacg tagacatcca
     3181 gccccagtcc ggcatgccgg ccgggggcga cgcgcggctg gctgcgctgc atggcccggc
     3241 gctgggcaaa catgcccgcc aggccgccgg cgggcgactc gacctcctgt ggcggctcct
     3301 gatgggtgaa aaccagcggc agcccgcgcg cggccgccag ccgcccaatg gcctcgcccg
     3361 cggtcagcat ggcttcgcgc accagttcgc ggctgcgcaa cggcggcagc ggcgtgatgg
     3421 ccacctgccc atcgtccctg acacgcacct tcacctccgg caggtcgatc tccaccgccc
     3481 cattggccac ccgccgggcg cgggccgcgc cggccaacgc cagcagctcg cgataggggc
     3541 tttcgcccag gcacgcttcg gcggcctcat aggtcgtccg cgtgacccgc acccagctag
     3601 ggacaatttc cagcagttcc ggcgcgtcgc cgccggcggc gaaagccagc gacagcgccg
     3661 gcgaaacctc ttgcaggccc agcgccagcc ggtcggtcaa ctcgtcgggc aacatccgcg
     3721 ccgtgccctc cggcagatag aggttggcgg cgcgggcacg ggcctcgcga tcggctggcg
     3781 agtcgggcgg cacgagggcg gcggcgtcgg cgatgtggac ccacagcacg ccgtcggcca
     3841 ggccgacggc gtcgtcgggg tcgctgctgc cctcgtcgtc gatggccagc gccagcaggt
     3901 gggtcaggtc gcggcgcggc tcgtctggca gcggcccgat gggcacgtcc acccggctca
     3961 cggccaggtt gagccgcgcc ggataggggt tgtcggccgg cgtccagacg ccggcggcca
     4021 gcagaaaggc gtgggcggcc tcgcgcgtct gcggcctgtc gaggcggcgc agcgtgcggc
     4081 tgctctcggt ctgatccagc gccagggcga ccacgtcggc caggtagggc gcgtcttcgg
     4141 gcgcggtctg gccggcggca gcgcgggcga caaaggcggc ccaggcgcgc tcctcggccg
     4201 cgcgggcctc gcgggcggcc agcgtctcgg ccaccttgct ggccgggtag acgatgatgg
     4261 cgtcgggcgc gccgccgaag tagaggccat cggccaccag ttgccaggca gcccaggccc
     4321 cggcgggcgt gtagtcgcca taggccagct cggccagttc gggcagggtc gtgtggccgc
     4381 tgtcgagcag ttcccaggcc gtcagcgggt cgccctccgg cggtggcagg cggcgcaggt
     4441 cggctgccgg gccggggtgc agcacggcca cgtccttggg ccgcacgttg acgcgctcgc
     4501 cgttgggcag ctcgatggtc agcttcttat cggtcgtcgc ccgcacgacc gccggctggg
     4561 ccttgtagag caccagggca tggagccgcg gctccatcag ccgccgcgcc ccttgaagtc
     4621 gtccatggtg tggttgatgc cgaagaagct gatcaccggg tcaaagaggg ccggcggcag
     4681 cagtcgcgtg gggaacagcg ccagcacgaa aggcggcagc accaggcgct gcttgttgcg
     4741 ctcgatggcg gccacgatgc ggctggccac atcgtccggc tccatgatcg gcagcaggaa
     4801 ggggaagcgc gtcttgacgc cgcggaacat gcccgtattg atgtagtagg gacagacaac
     4861 ggttgtgcgg atgccgcggt ggcccaggcg ctttagctcg aagcgcaagg actcgtcgaa
     4921 gccgatggcg gcgaacttgc tggcgctgta gtcggtcata cggggcacgc cgatcaggcc
     4981 gccggccgag gcgacagtga cgatgtggcc gctgccctgg cggatcatgc ccggcaggaa
     5041 gcagcgtgtc gtccggaaga gggccagcgt gttgatgtca aaggtgcgct tgatggcctc
     5101 gtcggtcagt tctaatagcg gcttgccgga gacgatgccg gcgttgttga tgagaatgtc
     5161 cacgtcgccg tgctcggcca ggacaaggcg cgccgtggtc tcgatctcct cgcggtcgct
     5221 gaggtcgcac tgctggtagg tggcggcgcg atagccccag tgttcgagtt ggtcggccag
     5281 ggcctccaac ccggcccggt cgatgtccca caagatgacg tggccgccgc gcgcagcgat
     5341 cttctgggcc atcagccggc cgatgccgct ggcgctgccg gtgatgagaa cgttcttgcc
     5401 tgcaaaactg gtcatgtgcc tcgcctcgtt gtgggggtta agtcgtgccc gatgaagtct
     5461 cattatagag gtagccgggc caaacgggca atcgttgcgc gccgggccgc caatcctcct
     5521 gtttacatgc gcttagccaa ctctttacct gttgttaact ctgtccaatt gacccgcacc
     5581 ctgcgaaacc ggtagaatcg gccccatgaa tacgaacagc tatgatatta gctctgacta
     5641 ccacatgcat tccaccttta gcatggatgg tcaggacaca atcgaggctc tcggctggcg
     5701 cgcgctggcc ctgggtttca acgagatcgc tatcaccgag cacgccgagt ggcatcccgc
     5761 ctggcgcggc tcgttcgacg tggacggcta cttcgccgaa atcgcccgct gccgggcgct
     5821 tttcgcacca caagggctga cgctttacag cggcgtggaa atgggcaacc cgcacgaatt
     5881 cgcccgcgag gcctcggccc tgacggccgc ctatccgttc gacgtgcgca tcgcctcgct
     5941 gcactggctc cacgaccaca acatccacga cagcgcctgc ttcgccgggc acgacccgct
     6001 ggatgtctat gccgactact tcgtcgccct cggccacatg gccgccgact tctccgatat
     6061 cgacgtgatc gcccacttcg accgcatcct ctggcggggc agcctgctgg gggcgacgtt
     6121 cgcgccgcgg gccattgaga acctggtgcg cgacgctctg gcgaccattg cctggcgcgg
     6181 gctggcgctg gaactcaaca cgcgcaacct taaccatatg cccaactggc ggccggcgct
     6241 gacgacgatg ctgcgctggt ttggggaaga gggcggccgt cgtgtcgtcg tcaactccga
     6301 cgcccaccgc gtcggtcaga tgggggccaa cctggaactg gccgcggctg tcctgcgcga
     6361 ggcgggactg gcggcgacga cgctgcaacc ggccctggtg gcagcggtta ggggatagag
     6421 gttccggggg agaagggggc agggggagag gctcacgcaa aggcgctaag gggcgaaggc
     6481 gcaaaggaag agaagaatct tgctcttctt cctttgcg
//
LOCUS       DAOSMQ010000134         6485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_11995_length_6485_cov_4.022551, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000134 DAOSMQ010000000
VERSION     DAOSMQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11995_length_6485_cov_4.022551"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK829_05935"
     CDS             complement(<1..222)
                     /locus_tag="PK829_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40789.1"
                     /translation="MGAKRARAFVFLLAAALLLAACRGRQGLTGPADLPTLAPTAAAT
                     AATPATAAPPAGSPTPNVPPTAAATLPPTF"
     gene            complement(228..620)
                     /locus_tag="PK829_05940"
     CDS             complement(228..620)
                     /locus_tag="PK829_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOU40790.1"
                     /translation="MNEAQFAAWLDAYGRAWQMGDAKAVMTLFAPAATYYETPFDPPL
                     VGLDAIHDYWRAGAGAAQRDVAFTYDVLAVAGDLGLAHWTATFTRVPSGAAVRLDGAL
                     AARFDAAGHCVEFREWWQRLEAPGAGPG"
     gene            751..2505
                     /locus_tag="PK829_05945"
     CDS             751..2505
                     /locus_tag="PK829_05945"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOU40791.1"
                     /translation="MTPNETDFQENLRAGQIALQAAAWAAARAAFARALAVLPQTDDP
                     SPGNIAPMAAPQAGAAVAASAHDGLGVALWWLNDIPAAHHHRTQAYLGFRQAGELGRA
                     ARIAAWLAREQVFLSGNVPGMMGWFQRANQLGTALPPGIDRAWLAILRASMLAGPAEM
                     TAIAAEVVATARAAGHGDLEAFALAFGGQAEVALGRVGQGLAHLDEAMIMATTGEVAD
                     LMTISEIFCVMLSACELAGDLARSEQWCHAGLEFARRYDCPFLLAYCRTSYGGLLSAL
                     GRWQEAEVALTEAIRAFERGHRGLRVQAVIKLAELRLSQGKLDEAGVLLAGLEDQAAA
                     VVPLARLWWRKGEPALARALLEQGLPQPAPPDATADSLYQLPALLLLTDILLATENAD
                     AVAPILARLTRLAEQAGSPLLSAQMAFTEGRVSLHRGDYAAARASFNAALGQLRSYEQ
                     SLLAGQVRLRMAETLRASDPAGAAAWARGALATFERIGAANDAAQAAAMLRGLGITGR
                     AGPRLHKPLTAREAEIAALIARGLSNREIAQRLVLSAKTVEHHVGRILSKLDLHSRAE
                     IAALAASGRLDALQEGES"
     gene            2611..3117
                     /locus_tag="PK829_05950"
     CDS             2611..3117
                     /locus_tag="PK829_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019544122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HOU40792.1"
                     /translation="MQAIDKQQHAVVRAGVDGRALWHLGALLHFKALGVETGGQFWAV
                     EGLADRHMAVPLHAHSREDEIWYVLEGTIRFTIGNETRDAGPGTFAYIPRGVAHTFQV
                     LSETARWFGIGTPAGLDDWFFETGEPAGALTLPPPGGPPDVEMIIATLRRYGTETLGP
                     PPGLASES"
     gene            complement(3220..3714)
                     /locus_tag="PK829_05955"
     CDS             complement(3220..3714)
                     /locus_tag="PK829_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016865123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOU40793.1"
                     /translation="MEHKTHQEKIETLRELIKDIDFAMLTTVDANGSLLSRPMSTQEA
                     EFDGDLWFFTSEDTGKVGDIRGDNRVNVAYARPDDHTYVSVSGTAELLNDRAKMEELW
                     SPVYKAYFPDGLDDPQLRLIKVTVDKAEYWKGHGLVRTVIGFAKALSGQESELGENEK
                     LQLG"
     gene            complement(3829..6396)
                     /locus_tag="PK829_05960"
     CDS             complement(3829..6396)
                     /locus_tag="PK829_05960"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU40794.1"
                     /translation="MSDKQVTLPVVGMTCANCVAAVERNAKKAAGVTDAAVNFASERV
                     TVNYDPDVAGGAQVTADVIDRIRRAGYDVPTAEVELPLLGMTCANCAATIERRLKKTD
                     GVVDATVNLASERATVRYAPGATTRADLVAAVRAAGYDVVEAAEGEEAVDAEAAAREA
                     AIAHQKRRLLIGVLFTLPLFLLSMSRDLGLLGSWASALWVDWLFLALATPVQFYVGWD
                     YYTGAFKSLRNGSANMDVLVALGSSVAYFYSVAVLLFNTAGSHAIGHHLYFETSAVII
                     TLIVTGKLLEVRAKGRTSEAIKKLMGLQSKTARVVRDGVERDIPIAEVVTGDRLLVRP
                     GEKIPVDGHVIEGRSSVDESMITGESLPVDKIAGDAVIGATVNRQGLLTVEAGKVGRD
                     TMLAQIIRMVESAQGSKAPIQRVVDRVAAYFVPAVILLALLTFAVWLLAGAGFVPALL
                     RLIAVLVIACPCAMGLATPTSIMVGIGKGAEAGILFKNSAALEEAHRLNAIVLDKTGT
                     ITRGEPAVTAVVTDHDRDQRSVVGELQAVASGAAAPPPAISAWPLAADDLLPLAAAAE
                     RGSEHPLGEAIVRAAEARGLALSSPQRFEAVAGHGIVATVDGQRVLVGNQRLLEREGV
                     APGAGLVERAAALEAAAQTVMWVAVDGRAAGLIGVADTIKDGSAEAVRALRDLGLTVV
                     MLTGDNPATAAAIGREAGIDRVLAGVLPGEKAEYVQRLQAEFGGDNHAAHIGMVGDGI
                     NDAPALAQANVGFAIGTGTDVAMETADVTLMRGDLRSVPQAIRLSQATMTNIRQNLFW
                     AFGYNVVLIPIAAGVLAPFDWAPDFLRQLSPILAAGAMAFSSISVVSNALRLRRARI"
BASE COUNT         1016 a         2267 c         2233 g          969 t
ORIGIN      
        1 gaaggttggc ggcagcgtcg ccgccgcggt cggggggacg ttgggtgtgg gcgagccggc
       61 gggcggggcg gccgtggccg gggtggcggc cgtcgccgca gccgtcgggg ccaacgtcgg
      121 caggtcggcc ggcccggtca ggccctgtcg cccacggcaa gccgccaaca gcagagcggc
      181 cgccagcaga aacacaaatg cccgcgcgcg cttggctccc atctccctca tcctggcccc
      241 gcgccgggcg cttcgagacg ctgccaccac tcgcgaaatt cgacgcagtg accggcggcg
      301 tcgaagcggg ccgctaacgc tccgtcgagc cgcacggccg cgccggaggg cactcgcgtg
      361 aacgtcgccg tccagtgggc gaggcccagg tcgccggcga cggccagcac gtcataggtg
      421 aaggccacgt cgcgctgcgc cgcgcccgcg cccgcccgcc aatagtcatg gatggcgtcc
      481 aggccaacca gtggcgggtc gaaaggtgtc tcgtagtaag ttgctgccgg ggcgaagagg
      541 gtcatgaccg ccttggcatc gcccatctgc caggcccggc cgtaggcatc gagccaggcc
      601 gcaaattgcg cttcgttcat cgcttagcct ccacagagga ttatagcgcg aagccgccat
      661 gtggcgcgac tctcccaagt cgcgccacgg caggatggct ctataataag ggggtcaagc
      721 gcgccacgcc ggcgcgtccg agttgcgccc atgacgccga acgagaccga cttccaggag
      781 aacctccgcg ccggacagat cgccctgcag gccgcggcct gggcggcggc acgggcggcc
      841 tttgcgcggg cgttggccgt gctgccccaa acagacgacc cgtcacctgg gaacattgcg
      901 cccatggccg ctccccaggc cggcgcggcc gtggcggcca gcgcccacga cggcctgggg
      961 gtcgccctgt ggtggctcaa tgacatcccc gctgcccacc accatcgcac acaggcctac
     1021 ctgggcttcc gacaggccgg cgagttgggc cgcgcggcgc gcatcgccgc ctggctggct
     1081 cgcgaacagg tcttcctgag cggcaacgtg ccgggcatga tgggctggtt ccaacgcgcc
     1141 aaccagttgg gaactgcgct gccgccaggt atcgaccgtg cctggctggc catcctacgc
     1201 gcctcgatgc tggccggccc ggcggagatg acggccattg ccgccgaggt cgtcgccaca
     1261 gcccgcgcgg cgggccacgg cgacctggag gctttcgccc tggccttcgg cggccaggcg
     1321 gaggtcgccc tcggccgcgt cggccagggc ctggcccacc tcgacgaggc catgatcatg
     1381 gccaccacgg gcgaagtggc cgatctcatg accatcagtg agatcttttg cgtcatgcta
     1441 tcggcctgcg aactggccgg cgatctggcc cgcagcgaac agtggtgcca tgccgggctg
     1501 gaattcgccc ggcgctacga ctgccccttc ctgttggcct actgccgcac gtcctacggc
     1561 ggcctgctga gcgcgctggg gcgctggcag gaggccgaag tagccctgac cgaagccatc
     1621 cgggcctttg agcgcggcca ccgcgggctg cgcgtccagg cggtcatcaa actggccgaa
     1681 ctgcgtctaa gccagggcaa gctcgacgag gccggggtgc tgctggccgg gctggaagac
     1741 caggccgcgg ccgtcgtccc cctggcgcgg ctgtggtggc gcaaagggga accggccctg
     1801 gcgcgcgcct tgctggaaca agggctgccg cagccagcgc cgccggacgc gacggccgac
     1861 tcgctctatc aactacccgc cctactactg ctgaccgaca tcctgctggc gacggaaaac
     1921 gccgatgccg tcgcgcccat cctcgcccgc ctgacccgac tggccgagca agcgggcagc
     1981 cccctgctgt cggcgcagat ggccttcacc gaggggcgcg tcagcctgca ccggggcgac
     2041 tacgccgccg cccgggccag cttcaacgcg gcgctgggcc agctacggtc atacgaacag
     2101 tcgctgctgg ccgggcaggt gcgactgcgc atggccgaga cgctgcgggc gtcggacccc
     2161 gccggggcgg ccgcctgggc caggggcgct ctggcgacct tcgagcgcat tggcgcggcc
     2221 aacgatgctg cccaggcggc ggcaatgctg cgcggtctgg gcatcaccgg tcgcgccggg
     2281 ccacgcctgc acaaaccact gaccgcgcgc gaagcggaga tcgccgccct catcgcccgt
     2341 ggtctgagta accgcgaaat cgcccagcgc ctggtactca gcgccaagac cgtcgaacac
     2401 cacgtcggcc gcatcctcag caaactggac ttgcacagcc gcgctgagat agccgccctg
     2461 gccgccagcg gccggctcga cgcgctacaa gaaggcgaaa gttaggggag ggactagggg
     2521 gatatccccg acgcgcagcg cggcgcgacc ggctatcctt gtggtcatac caacggccgg
     2581 gcgcacaggc ctggccacag gagaaatgac atgcaggcaa ttgacaaaca gcaacacgcg
     2641 gtcgtgcggg ccggcgtaga cgggcgggca ctctggcact tgggcgcgct gctccacttc
     2701 aaggcgctgg gcgtggagac gggggggcag ttctgggccg tggaagggtt ggccgaccgg
     2761 cacatggccg tacccctgca cgcccacagc cgcgaagacg agatttggta cgtgctcgaa
     2821 gggacaatcc gcttcaccat tggcaacgag acgcgcgacg ctggccccgg gaccttcgcc
     2881 tacatcccac gcggggtggc ccataccttt caggtccttt cggagacggc gcgctggttc
     2941 ggcatcggca cgcccgcggg actggatgac tggttcttcg agacgggcga gccggccggg
     3001 gcgctgaccc tgccgccacc cggcggcccg ccggatgtgg agatgatcat cgccaccctg
     3061 cgccgctacg gcacggagac gttagggccg ccgccagggc tggccagcga atcgtgagca
     3121 gaagagcgac aaacgctggc cccggacgac ggcctttttc aggtcgtcgt ccggggccgg
     3181 cgtatagggt cagcagcgac tccgtcgccg cctctctcac tatcccaact gcaacttctc
     3241 gttctcgccc aactccgact cctggccgga cagcgctttg gcgaagccga tgactgtccg
     3301 caccaggccg tgccccttcc agtattcagc cttgtcaacg gtgaccttga tcagccgcaa
     3361 ctgagggtcg tccagcccat ctgggaagta ggccttgtac actggcgacc acagttcctc
     3421 catcttggcg cggtcgttca gcaactcagc cgtgcccgag acggaaacgt aagtgtgatc
     3481 gtctggccgg gcgtaggcga cgttgacgcg gttgtcgcca cggatatcgc ccaccttgcc
     3541 ggtgtcctcg ctggtgaaga accacagatc gccgtcgaat tcggcttcct gggtgctcat
     3601 cggccggctc aacagcgagc cgttggcgtc gacggtcgtc agcatggcga aatcgatgtc
     3661 cttgatcaac tcgcgcaggg tttcgatctt ttcctgatgt gtcttgtgct ccatttgtgc
     3721 tcctccggca gtgccgtttg gggtaatgcg accatcgtag catttcaccc cgcccgtggc
     3781 cctatgccgg aggcacaaat aggaggcacg accgggtcac ccccgcggtc agatgcgcgc
     3841 ccgccgcagt cgcagcgcgt tgctaaccac gctgatgctg ctgaaggcca tcgccccagc
     3901 ggccaggatg gggctgagct ggcgcaggaa gtcgggtgcc cagtcgaacg gggccagcac
     3961 gccggcggca atggggatga ggacgacgtt atagccgaag gcccagaaca ggttctggcg
     4021 gatgttggtc attgtggcct gcgagaggcg gatggcctgg ggcacgctgc gcaggtcgcc
     4081 gcgcatgagg gtcacgtcag ccgtttccat ggccacatcc gtgcccgtgc cgatggcgaa
     4141 gccgacgttg gcctgggcca gcgccggggc atcgttgatg ccgtcgccga ccatgccgat
     4201 gtgggcggcg tggttgtcgc cgccgaactc ggcctgcaag cgctggacgt attcggcctt
     4261 ctcacccggg agcacgccgg ccagcacacg gtcgatgccc gcctcgcggc caatagccgc
     4321 ggccgtggcc gggttgtcgc cggtcagcat gacaacggtc agacccaggt cgcgcagggc
     4381 gcgcacggcc tcggccgagc cgtccttgat ggtgtcggcc acgccgatga ggccggcggc
     4441 gcggccatcg acggccaccc acatgaccgt ctgcgccgcg gcttccaacg ccgcggcccg
     4501 ctcgaccagg ccagcgccgg gcgccacgcc ctcccgctcc agcagccgtt ggttacccac
     4561 caacacgcgt tgcccatcga ccgtggccac gatgccgtgc ccggcgacgg cctcgaagcg
     4621 ctgcggcgac gatagagcca gcccgcgcgc ctcggccgcg cggacgatgg cctcgcccag
     4681 ggggtgctcg ctaccgcgct cagccgccgc ggccagtggc agcaagtcgt cggcggccag
     4741 tggccaggca gaaatggccg gtggcggagc ggccgcgcca ctggcaacgg cctgcaactc
     4801 accgacgacc gatctctggt cgcggtcgtg atcagtgacc acggccgtga cagccggctc
     4861 gccgcgggtg atcgtgcccg tcttatccag cacgatggcg ttgaggcggt gggcctcttc
     4921 cagcgcggcg ctgttcttga acaggatgcc cgcctcggcc cccttgccga tgccaaccat
     4981 gatcgaggtc ggcgtggcca ggcccatggc gcaggggcag gcgatgacca gcacggcgat
     5041 gagccgcagc agcgcgggca cgaagcccgc cccggccaga agccagacag caaaggtcag
     5101 cagcgccagg aggatgaccg cgggcacaaa gtaggcggcg acgcggtcga cgacgcgctg
     5161 gatgggggcc ttgctgccct gggccgactc gaccatgcgg atgatctgcg ccagcatcgt
     5221 gtcgcggccg accttgcccg cctcgacggt cagcagcccc tggcggttga ctgtcgcgcc
     5281 gatgacagca tcgccggcga tcttgtccac gggcaggctc tcgccggtga tcatgctctc
     5341 atcgaccgag gagcggccct cgatgacgtg gccatcgacc gggatcttct cgcccggtcg
     5401 caccagcagc cggtcgccgg tgacgacctc ggcaatgggg atgtcgcgct ccacgccgtc
     5461 gcggacgacg cgggcggtct tcgattgcag gcccatcagc ttcttgatgg cctcgctggt
     5521 gcgccccttg gcccgcactt ccagcagctt gccggtgacg atgagggtga tgatgacggc
     5581 cgaagtctcg aagtagaggt ggtggccgat ggcgtggctg ccggccgtgt tgaagagcaa
     5641 cacggcgacg ctatagaagt aggccaccga cgagccgaga gccacgagca cgtccatatt
     5701 ggcgctgccg ttgcgcaggc tcttgaacgc gccggtgtaa tagtcccaac caacgtagaa
     5761 ctgcaccggc gtggccaggg ccaggaacag ccaatcgacc cacagggcgc tggcccaact
     5821 gcccaacagc cccaggtcgc ggctcatgct cagcaagaag agtggcagcg tgaagagcac
     5881 cccgattagc agccggcgct tctggtgggc gatcgcggcc tcgcgggcgg cggcttcggc
     5941 atcgacggct tcctcgcctt cggccgcctc gacgacgtcg tagcccgcgg cgcggacggc
     6001 ggccaccaga tcggcccgcg tcgtcgcccc cggcgcatag cgcaccgtgg cccgctcgct
     6061 ggccaggttc accgtcgcgt ccaccacgcc gtcggtcttc ttcaggcggc gctcgatggt
     6121 cgcggcgcag ttggcgcagg tcatgcccag cagcggcagc tcgacttcgg ccgtgggcac
     6181 gtcgtagccg gcgcggcgga tgcggtcgat gacgtcggcc gtcacctgcg cgccgccggc
     6241 cacgtcgggg tcatagttga ccgtgacacg ctcggaagcg aagttgacgg ccgcgtcggt
     6301 gacgccggcg gccttcttgg cgttgcgttc caccgcggcc acacagttgg cgcaggtcat
     6361 gccaaccacg ggcagcgtta cttgcttgtc agacatttta cctcaggggc aagcccccgc
     6421 gccgggacgg gcggccgatg cgctgccctc cccggcgaca cgtgcgcctc ggtagcgcgc
     6481 tagtt
//
LOCUS       DAOSMQ010000135         6445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12116_length_6445_cov_5.115180, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000135 DAOSMQ010000000
VERSION     DAOSMQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12116_length_6445_cov_5.115180"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..573
                     /locus_tag="PK829_05965"
     CDS             160..573
                     /locus_tag="PK829_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOU40795.1"
                     /translation="MINGVVAQKLQALDRVLTELRSLGVVSVKDLHANWQQARAIERN
                     LQVLVEVVIDVCQRLIAVAGQTPATTGADAVARCIELGVLSADENYTRMVRLRNFLVH
                     RYEHVDPEVLANMVNRHLGDFEQFRDEVLTYVQNH"
     gene            557..946
                     /locus_tag="PK829_05970"
     CDS             557..946
                     /locus_tag="PK829_05970"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOU40796.1"
                     /translation="MSRIIELDALLPVWKNAPDVVAAWAFGSAQDGQVRPGSDLDIGV
                     LMARPPTFDEQLDLLGRLQDTLGLDEVDLVILNDANPILRFEAVSGRRLFTRDLLALA
                     EFVSLTAREYEDEMAQYERALSQRGAV"
     gene            1036..4128
                     /locus_tag="PK829_05975"
     CDS             1036..4128
                     /locus_tag="PK829_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOU40797.1"
                     /translation="MGSNWKRFIIIVIVVLASVALWGARPGPAQAQNAPFPYKEVTDA
                     AGIQGPRVGNEKITGQAWGDYDRDGWIDLYLTDFGADKKPAANRLYRNNGDGSFSLSP
                     LSSQVALPTAQSGGAVWADYDNDGFLDLYVVNWGANVLYRNDAGQGFTDVTAAAGVGD
                     TANGKSASWGDYDNDGSLDLYVANWSCTPRCGRPVEGDMDVLYHNNGDGTFTDVHSLL
                     GRQTAGAGFIASFIDFDNDNDLDIYLVNDEFVHAVGNALWRNDGAGCGGWCFTDVSAE
                     AGVDQKVMGMGLAVGDYDNDLDLDFYFSNVGPSTLLQNQGDGTYTDVAEAAGVRMPEA
                     IGWGTIFLDYDNDGRLDLYMSQMVETDGGVGLNPLFHNNGDGTFTNLLEASGASDPGK
                     SIGVSAADYDADGYLDIIIGDYDEGYKLFRNTLGDAETGGGWFALRLDGAAGVNRDAI
                     GAKVFVTDSDGHTQMQQLNNGTGLGGNSGLELHFGLGAARLAAVRVVWPDGAAQSFDD
                     LRANTRYTLTYGGEPAVFSGRAAFHWPAWVATVLPWAVGALGLAALVVVAAVLRRRGR
                     RPHGRRGLRPALAAMGLIVLAGAAALLWTTRDTLAARLGGGSSDGRLTLLLNKSGVTQ
                     LDFGPTPDPALVALGEALFFDKLLSGNRDISCATCHHPDFASADGLPLSFGTGGHGLG
                     PVRAIGVDRELVPRNATELFDRGSPEWTTMFWDGRVNLSAVYGLSTPVGNGLPPLLET
                     ALEAQAMFPVTSRDEMRGQFGDYDAGRQLNEIAAVSDMRPKLIWARLMKRLLAVPEYV
                     ELFRAAYPDVPIEQLGFEHAAKAIAAYEASAFTFTDSPWDRYLAGDRAALSDQAKRGA
                     ILFYGEAGCAACHSGNLLTDQQFHNVGVPQLGPGKLSDERLDLGRFLETGRAEDRYAF
                     RTPPLRNVTLTAPYMHNGAYADLRAAVRHHLNPPGSLQVYDAATLPVSFQETVRTEPE
                     VAADILKTLDVRLATPAALTDAQLADLMTFLAALTSPTAVDLSGLVPASVPSGLPVQD
                     "
     gene            4367..4915
                     /locus_tag="PK829_05980"
     CDS             4367..4915
                     /locus_tag="PK829_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOU40798.1"
                     /translation="MTTQTKKTNPVKTNFLVDIAIFVAFLIALEPRLTGMAIHEWLGI
                     AFGAAIITHLVLHWKWLVATTKRFVGRLPGSTRMNYIINALFFINMTVLVFSGLMISE
                     TALPALGIQLGEGRSFERLHMLTADWALYLLALHVALHWRWIVSTTNRYVVQPLLRPF
                     RKQHQSQPVAALSLSTLEEAQS"
     gene            4912..5310
                     /locus_tag="PK829_05985"
     CDS             4912..5310
                     /locus_tag="PK829_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40799.1"
                     /translation="MKLLGKIVVILMAALMVVGGTLALEKNGALSSLTAGGAGEQGEG
                     APADFDGAFAPGQRPERGDGDFGGEGGRDGAGSLMSIGRNLGTIAAIVAGVALLGWLA
                     RQTSQATNRSKLAKAAMPPDKEVTKPDEFL"
     gene            5515..>6445
                     /locus_tag="PK829_05990"
     CDS             5515..>6445
                     /locus_tag="PK829_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU40800.1"
                     /translation="MANCANCGTANPDGAKFCLNCGTKLAQACPACGTELPAGARFCL
                     NCGHRVTAGAPAAEAAPPPPAPPAALDRFMPRELAQKLNQARATGEMLGERRVVTMLF
                     CDVKGSTAAAARFDPEEWAEIINGAFEPMIGPIYRYEGTVARLMGDGLLAFFGAPIAH
                     EDDPQRAVLAGLEILEGIRHYREAVQRQWGLDLDVRVGVNTGLVVVGAVGSDLRMEYS
                     ALGDAINIAARMEQTAQPGTVQITPATYKLVAPLFDFETIADLEVKGRAEPMTAYRVL
                     GRRAAPGATRGLAGLSSPLVGREAELGQVVAALT"
BASE COUNT         1069 a         2166 c         2130 g         1080 t
ORIGIN      
        1 aggggtggtg gccctgcgca cggcgacgcg gctggaaggg gtgctggtcg agcggttgct
       61 ggcccaaccg ggcgtcgttc cgctggctcc cggcctgggc ggcagcgacg accgcggccg
      121 gacgcacctg ttacgggcag cgtatggtat aattgacctg tgatcaatgg cgtcgttgcc
      181 cagaaactac aggcgctcga tcgtgtgctg acggaactgc ggtcgttggg cgtcgtctcg
      241 gtcaaagacc tgcacgccaa ctggcaacag gcgcgggcca tcgagcgcaa tctgcaagtg
      301 ctggtcgagg tcgtcatcga cgtttgccag cggctcattg ccgttgccgg ccagacgccg
      361 gcgaccacgg gggccgacgc cgtcgcgcgt tgcatcgagt tgggcgtact gtcggccgat
      421 gagaactaca cgcgcatggt gcgcttgcga aacttcctcg ttcaccgtta tgagcatgtc
      481 gatcccgaag tgctggccaa tatggtcaat cgacacttgg gcgacttcga acagttccgc
      541 gacgaggtgc tgacctatgt ccagaatcat tgaactcgat gccctgttgc cggtctggaa
      601 aaacgcgccc gacgtcgtgg ccgcgtgggc cttcggctcg gcccaggatg gacaggtgcg
      661 cccgggcagt gacctggaca tcggtgtctt gatggcgcga ccgccgacgt tcgatgagca
      721 gcttgatctg ctcggccgcc tgcaagacac gctggggctg gacgaggtcg atctcgttat
      781 cctgaacgac gccaacccca ttctgcgctt cgaggcggtg tcgggccggc ggctgttcac
      841 ccgcgacctg ttggcgctgg ccgagtttgt ctcgctgacg gcgcgggagt acgaagacga
      901 aatggcgcaa tatgaacggg cgctatcgca gcgcggcgcg gtttagcgct gccgggcagc
      961 cggcgcgacg gcgcgccgcc ggctgccgtt tgttgcccgt tgggggttat gggagcgccg
     1021 tcaaagaggt ctgtcatggg aagcaactgg aaacggttca tcatcatcgt catcgttgtt
     1081 ctggcgagcg tggccctgtg gggggcgcgg ccaggcccgg cgcaggccca gaacgcgcct
     1141 ttcccttata aggaagtcac cgacgcggcc ggcattcagg ggccgcgcgt cggcaacgag
     1201 aagatcaccg gccaggcctg gggcgactac gaccgcgacg gctggatcga tctctacctg
     1261 accgacttcg gcgccgacaa gaagccggcc gccaatcgcc tctaccgcaa caacggcgat
     1321 ggatcgttca gcctgtcgcc gctgtccagc caggtggccc tgcccacggc gcagagcggc
     1381 ggcgcggtct gggccgacta cgacaacgac ggcttcctcg acctctacgt cgtcaactgg
     1441 ggcgcgaacg tgctctatcg taacgacgcc ggccagggct tcaccgacgt aaccgccgcg
     1501 gccggcgtgg gcgacacggc caacggcaag agcgcttcgt ggggcgacta tgacaacgac
     1561 ggctccctcg acctctacgt cgccaactgg tcgtgtacgc cgcgctgcgg ccgcccggtc
     1621 gagggcgaca tggacgtgct ctaccacaac aacggcgacg gcacgttcac cgatgtccac
     1681 agcctgctgg gccggcagac ggccggcgcg ggcttcatcg ccagcttcat cgacttcgat
     1741 aacgacaacg acctcgacat ctacctggtc aacgacgaat tcgttcacgc cgttggcaac
     1801 gctctgtggc gcaacgatgg cgcgggctgc ggcggctggt gcttcactga cgtgtcggcc
     1861 gaggccgggg tcgatcagaa ggtcatgggc atgggcctgg ccgtgggcga ctacgacaac
     1921 gacctcgacc tcgacttcta tttctccaac gtcggcccgt caacgctctt gcagaatcaa
     1981 ggcgacggca cgtataccga cgtggccgag gcggccggcg tgcgcatgcc ggaggccatc
     2041 ggctggggca cgatcttcct cgactacgac aacgacggcc gcctcgacct ctatatgtcc
     2101 cagatggtgg agacggacgg cggcgtgggc ctcaacccgc tgttccacaa taacggcgac
     2161 ggcaccttca ccaacctgct ggaggccagc ggcgcaagcg accccggcaa gtctatcggc
     2221 gtcagcgccg ccgactacga tgccgacggc tacctcgaca tcatcatcgg cgactacgac
     2281 gagggctaca agctcttccg caacacgctg ggcgacgccg agacgggcgg cggctggttt
     2341 gccctgcggc tcgatggcgc ggccggcgtc aaccgcgacg ccatcggcgc gaaggtcttc
     2401 gtcaccgata gcgacggcca tacgcagatg cagcagctta acaacggcac cggcctgggc
     2461 ggcaatagcg gcctggagct gcacttcggc ctgggcgcgg ccaggttggc cgccgtccgc
     2521 gtcgtctggc ccgacggcgc ggcgcagtcg ttcgacgacc tgcgggccaa cacgcgctat
     2581 acgctgacct acgggggcga accggccgtc ttctccggcc gcgccgcatt ccactggccg
     2641 gcctgggtgg cgacagtcct gccctgggcg gtgggcgcgc tggggctggc ggcgttggtc
     2701 gtggtcgcgg ccgtgttgcg gcggcgcggg cggcggcccc acgggcggcg cgggctgcgg
     2761 ccggcgctgg cggcgatggg cctgattgtg ctggccggcg cggcggccct gctgtggacg
     2821 acgcgcgaca cgctggccgc ccgcctgggt ggcggcagca gcgacggccg gttaacgctg
     2881 ctgctcaaca aatcaggcgt tactcagctc gacttcggcc ccacgcccga cccggcgctg
     2941 gtggccctgg gcgaggcgct gttcttcgac aaacttctca gcggcaaccg cgatatctcc
     3001 tgcgccacct gccaccaccc cgacttcgcc tcggccgacg gcctgccgct gtcgttcggc
     3061 acgggcggcc acggcctcgg cccggtgcgc gccattggcg tcgaccgcga actggtgccc
     3121 cgcaacgcta cggagctatt cgaccgcggt tcgccggagt ggacgacgat gttctgggac
     3181 ggccgggtga atctgagcgc cgtctatggc ctcagcacgc cggtgggcaa cggcctgccg
     3241 cccctgttag agacggcgct ggaagcacag gccatgttcc cggtcacttc gcgcgacgag
     3301 atgcgcggcc agttcggcga ctacgacgcc ggacggcagt tgaacgagat cgccgccgtc
     3361 agcgacatgc ggccgaagct catctgggcg cggctgatga aacggctgct ggctgtgccg
     3421 gaatacgtcg agctattccg cgccgcctat cccgacgtgc ccatcgagca attgggcttc
     3481 gagcacgccg ccaaggccat cgccgcttac gaagcgtccg ccttcacctt caccgatagc
     3541 ccctgggatc gctatctggc cggtgatcgc gcggcgctga gcgaccaggc caagcggggc
     3601 gcgattctct tctatggcga ggctggttgc gccgcctgcc acagcggcaa cctgctgacc
     3661 gaccagcaat ttcacaatgt tggcgtgccc caactcggcc cgggcaagct gagcgacgaa
     3721 aggcttgacc tcggccgctt tctggagacg ggccgcgccg aagaccgcta cgcctttcgc
     3781 acgccgccgc tgcgcaacgt caccctgacc gcgccctaca tgcacaacgg cgcctacgcc
     3841 gacctgcgcg ccgccgtccg ccaccatctt aacccgcccg gctcgctgca agtctatgac
     3901 gccgcgacgc tgccggtcag cttccaggag acagtgcgca cggagccaga agtggcggcc
     3961 gacatcctca agacgctcga tgtccgtttg gctacgccgg cggcgctgac cgacgcgcag
     4021 ttggccgacc tgatgacttt cctggcggcg ctgacctcgc ccacggccgt cgatctgagc
     4081 ggcctcgtgc cggccagcgt gcccagcggc ctgccggtgc aggattaggc ggcgacggcc
     4141 gcctttggcg ggcggccaac acccccagta gccacccagg gccattgccg ggccgcaaga
     4201 agcgctcacg caaaggcgcc tttgcgtgag cgcctgttgg cctggcaata gccccggtaa
     4261 cccaccaatc agtccctata cacaaacaca acattgtctt cataactcct gaataaccgg
     4321 ccactatcat cctcgaagct gacctaagta aatgaggatg atagatatga ccacgcaaac
     4381 caaaaagacc aatccggtca aaaccaattt cctggtagac atcgctatct tcgtagcctt
     4441 cctgatcgcg ctggaaccgc gcctgaccgg gatggccatt cacgagtggc tgggcatcgc
     4501 ctttggcgcg gccatcatca cccacctggt gctgcactgg aagtggctgg tggcaacgac
     4561 gaagcggttt gtcggtcgcc tgcccgggag cacgcgcatg aattacatca tcaatgccct
     4621 gttcttcatc aatatgacgg tgctggtctt ctccgggttg atgatctcgg agacggcgct
     4681 gcctgcgctg ggcatccagt taggcgaggg acgttcgttc gagcggctac acatgctgac
     4741 cgccgactgg gcgctctacc tgctggccct gcacgtggcc ctgcactggc gatggatcgt
     4801 gagcaccacc aaccgctacg ttgtccagcc gttgctccgg ccgttccgca agcaacatca
     4861 gtcacaaccg gtggcggccc tgtcgctgtc caccctggag gaagctcaat catgaagctg
     4921 ttaggcaaga tcgtcgtgat tctgatggcg gctctcatgg tcgtcggggg cacgctggcg
     4981 ctggagaaga atggcgcgct gagtagcctg acggccggtg gcgctggcga gcagggcgag
     5041 ggcgcgccgg ccgatttcga tggcgctttt gcccccggcc agcgccccga gcgtggcgat
     5101 ggcgacttcg gtggcgaagg cgggcgcgac ggggccggca gcctgatgag cataggccgc
     5161 aacctgggca cgatcgccgc catcgtcgcc ggggtggcgc tgctcggctg gctcgcccgt
     5221 cagaccagcc aggccacgaa ccggagcaag ttggctaagg cggccatgcc gccggacaag
     5281 gaggtcacaa agccagacga gttcctataa accgatagac cccggtcaca tggtggacag
     5341 atggcacgga ttccctggtg gtgtccgtgt catttttgtt gccgcccaga ccactgctgg
     5401 gctgtccatt tctggtcact atctgagcag caaatggtct gtcatggccc ggcggccggc
     5461 tatacaatgg actctatact tccttgccga tggcggagga aaggagcgtg tcctgtggcc
     5521 aactgcgcga actgtggaac ggctaacccc gacggggcca agttctgtct caactgtggc
     5581 acaaagctgg cgcaggcctg ccctgcctgt ggcacagaac tgccggccgg cgccaggttc
     5641 tgccttaact gcggccaccg ggtgacggcc ggcgcgccgg cagccgaagc cgcgccgcca
     5701 ccccccgcgc ccccggccgc gctcgaccgc ttcatgcccc gcgaactggc ccaaaagctc
     5761 aaccaggcgc gggccacggg cgagatgctc ggcgaacggc gcgttgtcac catgctcttc
     5821 tgcgacgtca aaggctccac ggccgccgcc gcccgcttcg acccggaaga gtgggctgag
     5881 atcatcaacg gcgctttcga gccgatgatc ggccctatct accgctacga aggcaccgtt
     5941 gcccgcctga tgggcgacgg cctgctggcc ttcttcggcg cgcccattgc ccacgaggac
     6001 gatccgcagc gcgctgtcct ggccgggctg gagattctgg agggcatccg ccactatcgc
     6061 gaagcagtcc agcgccagtg gggcctagac ctggacgtgc gtgtcggcgt caacactggt
     6121 ctggtcgtcg ttggcgcggt cggctccgac ctgcgcatgg agtactccgc cctcggcgac
     6181 gccatcaaca tcgccgcgcg catggagcag accgcccaac cgggcacggt gcagatcacc
     6241 ccggcgactt acaagctggt cgcgccgctg ttcgattttg agacgattgc cgacttggag
     6301 gtcaagggcc gggccgagcc gatgacggcc taccgcgtgc tgggccggcg ggccgcgccc
     6361 ggcgccacac gcggtctggc cggcctcagc tcgccgctgg tcggccgcga ggcggaactg
     6421 ggccaggtcg tcgccgcgct gaccg
//
LOCUS       DAOSMQ010000136         6389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12259_length_6389_cov_5.384907, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000136 DAOSMQ010000000
VERSION     DAOSMQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12259_length_6389_cov_5.384907"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="PK829_05995"
     CDS             complement(<1..2266)
                     /locus_tag="PK829_05995"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU40801.1"
                     /translation="MPHPHPIEAEEPSCLELLDEAIADQKGIINVAVDTAEETILFAY
                     DPRRVSEDDILRVAHEVAPALQQRWDRCTLRLEKRGGRACESCALALEQQVGRLPGVR
                     QATASFAGGVMAVHYDSGLISISDITQRVEQFGAGVRPSAATRVAAPPAPTTRLGRAR
                     VWLTPQRLQALLTAVTFVAMIAAWLLARAAAGPAWLAPALFAVAYVAGGAFGLKGGVE
                     SLMARTLDIDLLMILAALGAAVVGQPFEGAMLLFLFSLSNTLQDFALDRTRNAIRALM
                     KLRPDEAAVYRGDRLVTLPIEQVRVGDRMLVKPGDRIALDGTVLTGAGSVDQASLTGE
                     SMPVYKKPGDPCFAGTINQDGSLEIGVTKLAKDSTLARLIQLVEEAQSEKAETQRFID
                     RFEQWYALGVIVLTALVATVLPLAFGQSWNAAFYTAMTVMVAASPCAIVISTPATVLS
                     AIGNGARRGVLFKGGIHVENAATVRAVAFDKTGTLTEGKPVVTDIVLMADAGVDAREE
                     VNAKTPRREDARGNDAVALARPGGLALASEEELLRVAAAVEARSQHPLAAAIVAEASA
                     RGLALLPVEHFQSVAGQGVRGVVADPAHGPLTIHIGNARYFDSYGLDGASDAASARVV
                     ALEEQGKTSVIVAAERPATAAGAAPLVLLGIIALADVLRPDAPAVVRELKAAGVERII
                     MLTGDNKRVARRIAAQAGVDEVFADLLPEDKVNAIRHVRDDFGPVAMVGDGVNDAAAL
                     AAATIGIAMGAAGTD"
     gene            complement(2453..2752)
                     /locus_tag="PK829_06000"
     CDS             complement(2453..2752)
                     /locus_tag="PK829_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40802.1"
                     /translation="MKTLSRIALILLAAVLIAGATYALAQSGWADSFTNSVIGANQAQ
                     MTATHDALQAAGAQPESHGEYFAMAGNLLKIALVAAGVILLSVVLEKRAKLRLAV"
     gene            complement(2749..3267)
                     /locus_tag="PK829_06005"
     CDS             complement(2749..3267)
                     /locus_tag="PK829_06005"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOU40803.1"
                     /translation="MATTRKANPTRVNLWLDVLMLVVFLIVFDPRLTGGTIHEWLGLA
                     IGATVIVHLLRHWNWIAQVTRRFLGRTTAAARLNYLVDALFFVALTLIILSGLMISEV
                     VLPFVGLSGAESPFWHLLHNMAANWAVALVAIHTALHWKWVVNTVRRTVLRPASVAGR
                     AVVGRAQKEVTP"
     gene            complement(3272..3862)
                     /locus_tag="PK829_06010"
     CDS             complement(3272..3862)
                     /locus_tag="PK829_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40804.1"
                     /translation="MKPDRRLLSRREFLIAAGCGCAATVLAGSGCARNSAAAADTVVC
                     PYGYVYDTWPGQCPRYVDSNGSGYCDYSDPIVATTPIVATTPIVATTATTLQTTDSAL
                     TTATDTAVVGTVAAPPATATAPATPAATTTAPTATAVAPTATAVALVILCNRGCRAPG
                     RCGRFTDNNGTGICDLSEGIPADQAAQYAQPSGRGG"
     gene            complement(3963..4712)
                     /locus_tag="PK829_06015"
     CDS             complement(3963..4712)
                     /locus_tag="PK829_06015"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020755091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOU40805.1"
                     /translation="MKLENKVALVTGAGSGIGRAIAERFVQEGARVLAADIRGDRLDE
                     VSAGLNAAGAFTTFTGSVGDRADNARMMDAVMDTYGRLDIVVNNAGIMDDFYPLAELD
                     DALWERVMAINLDGPMVICRRALQIMLPQGGGSIVNVASIGGLFGGRAGVAYTTSKHA
                     LIGLSRSVAFHYAQQGIRCNVIAPGGVATNIEVKNPSMFGYQRLQTTLAMVPRQGQPG
                     ELAAAALFLASDDASFVNGDVLVVDGGWTAG"
     assembly_gap    5064..5163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5164..5460
                     /locus_tag="PK829_06020"
     CDS             <5164..5460
                     /locus_tag="PK829_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOU40806.1"
                     /translation="MEERQIGTTELRQRLTDVIQAVKEEKVAYVVETFGRPQVVIVDA
                     NEYQSLKTYHAMRQSFFERLLVTGEANAKINADLTEEQLLALIDAARDEVYDEG"
     gene            5472..6005
                     /locus_tag="PK829_06025"
     CDS             5472..6005
                     /locus_tag="PK829_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOU40807.1"
                     /translation="MLRVVIDTSTLVSFALTSGEITSQIIRAWQAEEFVLLTTAAMRN
                     ELRRVLDRPRIRTRAEAPLTWIADELEHHSWHISGQLTVTGVGRDPKDDVFLACAVEG
                     QADYLVSSDRDLLDMHHYQDICIVNPGQFLVGLQMNRLTVEQIVARFSPAALEAVQST
                     LCLNRDAAVKVAQAMGR"
     gene            6005..>6389
                     /locus_tag="PK829_06030"
     CDS             6005..>6389
                     /locus_tag="PK829_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU40808.1"
                     /translation="MLPALLDHLRFNRDFMANVVAWERIPPRPARTAPLPDWLDPRLA
                     AALRGRGIDQLFSHQAAAIEAVRRGENVIVSTATASGKSLAYTLPILQRLLERPDARA
                     LYLFPTKALAHDQEAETASLVAAARL"
BASE COUNT         1079 a         2043 c         2117 g         1050 t
ORIGIN      
        1 cgtccgtccc ggccgcgccc atggcgatgc cgatggtcgc cgcggccagc gccgcggcgt
       61 cgttaacgcc gtcgccaacc atggccaccg ggccgaagtc gtcgcgcacg tgacggatgg
      121 cgttgacctt gtcctccggc agcaggtcgg cgaagacctc gtccacgccc gcctgggcag
      181 cgatgcgccg ggcgacgcgc ttgttgtcgc cggtcagcat gatgatccgc tcgacgccgg
      241 cggctttcag ttcgcgcacc accgctgggg cgtccggccg cagcacgtcg gccagagcaa
      301 tgatgcccag cagcaccagc ggcgcagcgc cggcggcggt ggccggcctt tccgcggcca
      361 cgatcacgct ggtcttgccc tgttcttcca gggcgaccac acgggcgctg gcggcgtcgc
      421 tggctccgtc caggccatag ctgtcgaagt agcgcgcgtt gccgatgtgg atggtcagcg
      481 ggccgtgggc ggggtcggcc acgacaccgc ggacgccctg cccggcgacg gattggaagt
      541 gctccacggg cagcagcgcc agcccacgcg cggacgcttc ggcgacaatg gccgcggcca
      601 gcgggtgttg cgagcgcgcc tccacggccg cggccacgcg caacaactcc tcttccgacg
      661 ccagcgccag cccgcccggc cgcgccaacg ccaccgcgtc attccccctg gcgtcttcgc
      721 gtcttggcgt cttggcgtta acctcttccc tggcgtcaac tccggcgtcc gccatcaaaa
      781 cgatatcggt caccaccggc ttgccctcgg tcagcgtgcc ggtcttgtcg aaggcgacgg
      841 cgcggacggt ggcggcgttc tccacgtgga tgccgccctt gaacagcacg ccccgccgcg
      901 ccccgttacc gatggccgac aggacggtgg ccggggtact gatgacgatg gcgcagggcg
      961 aggcggcgac catgacggtc atggctgtgt agaaggccgc attccacgac tggccaaagg
     1021 ccagcggcag caccgtggcc accagcgccg tcaggacgat gacgcccagc gcgtaccact
     1081 gctcgaaccg gtcgatgaag cgctgcgtct cggccttctc gctctgggcc tcctccacga
     1141 gttggatgag ccgggccaga gtcgagtctt tggccagctt ggtgacgccg atctccaggc
     1201 tgccgtcctg attgatcgtc ccggcaaagc acgggtcgcc cggcttcttg tagacgggca
     1261 tcgactcgcc cgtcagcgag gcctgatcga cgctgcccgc gccggtcagc acggtgccgt
     1321 cgagggcaat gcggtcgccc ggcttcacca gcatccggtc gccgacgcgc acctgctcga
     1381 tgggcagggt gacaaggcgg tcgccacgat agacggcggc ttcgtctggc cgcagcttca
     1441 tcagggcgcg gatggcgttg cgcgtccggt cgagggcgaa gtcttgcagc gtgttcgata
     1501 gcgagaagag gaacagcagc atggccccct cgaacggttg gccgacgacg gccgcgccca
     1561 gggcggccag gatcatgagc aggtcgatgt cgagcgtgcg ggccatgagc gattcgacgc
     1621 cgcccttcag cccaaacgcg ccgccggcca cgtaggccac ggcgaacagg gccggcgcca
     1681 gccaggccgg gccggcggcc gcgcgcgcca gcagccaggc ggcgatcatg gccacgaagg
     1741 tgacggcggt caggagcgcc tggagccgct gcggcgtcag ccagacgcgg gcgcggccga
     1801 ggcgggtcgt cggggctggc ggcgcggcaa cccgggtcgc cgccgacggc ctcacgccgg
     1861 caccgaactg ctccacgcgc tgggtgatgt cgctgatgct gatcaggccg ctgtcgtaat
     1921 ggacggccat gacgccgccg gcgaagctgg ccgtggcctg gcgcacgccg ggcaggcggc
     1981 cgacctgctg ctccaacgcc agcgcgcacg actcgcacgc ccggccaccg cgcttttcca
     2041 gccgcagcgt gcagcgatcc caacgctgtt gtagcgccgg ggcaacctcg tgggcaacgc
     2101 gaagaatgtc gtcctcgctg acgcgccggg ggtcataggc gaagagaatc gtctcttcgg
     2161 ctgtgtccac ggccacgttg atgatgcctt tttggtcagc gatggcttcg tcgaggagtt
     2221 caagacagct cggctcctcg gcctcgatgg gatgggggtg tggcatggct ggttccctca
     2281 gttgggggcg gttggggtgg gccgccaggc agggtcgtcg gccggcgcgt caccgtcctc
     2341 atagccaatt gccctttggg cgcacgcgcc tatcaaaggg caactttatt cttaatcagc
     2401 gttactgttg aatttaacgc tggttaaatt atagccgggg cggggcgaga agtcaaacgg
     2461 caagtcgcag cttagcccgc ttctccagga cgacgctcag gaggatcacg cccgcggcga
     2521 cgagggcgat cttgagaaga ttgccggcca tggcgaagta ctcgccgtgg ctctccggct
     2581 gcgcgccggc ggcttgcagg gcgtcgtggg tggcggtcat ctgcgcctgg ttcgcgccga
     2641 tgacgctatt ggtgaagcta tcggcccaac cgctctgcgc caacgcgtag gtcgccccgg
     2701 caatgaggac ggcggccagc aagattaggg cgatgcgaga taatgttttc atggcgtaac
     2761 ctctttctgt gcgcgcccga cgacggcccg cccggcgacg ctcgccggcc gcagcacggt
     2821 gcgacggacg gtgttgacaa cccatttcca gtgcagcgcc gtgtggatag cgacgagcgc
     2881 cacggcccag ttggccgcca tgttgtgcag cagatgccag aagggcgact ccgcgccgct
     2941 cagccccaca aagggcagga cgacctcgga gatcatcagg cccgacagga tgatcagcgt
     3001 cagggcgacg aagaacagcg cgtctacaag atagttcagt cgtgccgcgg ccgtcgtccg
     3061 ccccaggaag cgcctggtca cctgcgcgat ccagttccag tgccgcagca ggtggacaat
     3121 gaccgtcgcg ccgatggcca ggcccagcca ctcgtggatc gtccccccgg tcagccgcgg
     3181 atcgaaaacg atcaggaaga cgaccagcat gagcacgtcg agccacaggt tcacccgcgt
     3241 cgggttggct ttgcgcgtcg tggccatggc gttagccccc ccgcccgctg ggctgggcgt
     3301 attgcgccgc ctgatcggcg ggaatgcctt cgctcaggtc acagatgccg gtgccgttgt
     3361 tgtcggtgaa acgcccgcag cggccgggcg cgcggcaacc gcggttgcag agtatgacga
     3421 gagcgacagc ggtcgccgtc ggggcgacag cggtggccgt tggggcagtg gtggtggccg
     3481 cgggggttgc cggagcggtg gccgtcgctg gcggggcggc gacggtgccg acaacggccg
     3541 tgtcggtcgc cgtcgtgagg gctgaatcag tcgtttgtag ggtcgtcgcc gttgtggcca
     3601 caatgggagt tgtggccaca atgggagttg tggccacaat gggatcggag taatcgcagt
     3661 aaccgctgcc gttgctatcg acgtagcgcg ggcactgccc cggccaggtg tcatagacgt
     3721 agccgtaggg gcagacgacg gtgtcggcag cggcggcgct attgcgcgca cagccgctac
     3781 cggcaagtac cgtcgcggca caaccacacc cggcggcgat gagaaactcg cgccggctca
     3841 gcaatcttcg gtctggtttc ataaggtggc tccttctgaa aagcaatcta ctttccagag
     3901 ggtagccgcc cgccgttcag aagatgtgaa gaagtggttg gctttgtgtt aggggatagg
     3961 gcttagccgg ccgtccagcc gccgtcgacg accagcacgt caccgttgac gaagctggcg
     4021 tcgtcggagg ccagaaagag cgccgccgcg gccagttcgc caggctggcc ctggcgcggc
     4081 accatcgcca gcgtcgtctg caagcgctgg tagccgaaca tgctggggtt cttgacctcg
     4141 atgttggtgg ccacgccgcc gggggcaatg acgttgcagc ggatgccctg ctgggcatag
     4201 tggaaggcga cgctgcgcga cagaccgatg agggcgtgct tcgaggtggt ataggccacg
     4261 ccagcccggc cgccgaacag gccgccgatg gaggcgacgt tgacgatcga accgccgccc
     4321 tgcggcagca tgatctgcaa ggcccgccgg cagatgacca tcggcccgtc gaggttgatg
     4381 gccataaccc gctcccacag ggcatcgtcc aactcggcca gggggtagaa gtcgtccata
     4441 atgccggcat tgttgacgac gatgtccagc cggccgtagg tgtccatgac cgcgtccatc
     4501 atgcgggcgt tgtcggcccg gtcgccaacg ctgccggtga aggtggtgaa cgcgccggcc
     4561 gcgttcagcc ccgcgctgac ctcgtccaac cgatcgccgc ggatatcagc ggccaggacg
     4621 cgcgcgcctt cctgaacaaa tcgttcggca atggcccggc cgatgccgga gcccgcgccc
     4681 gtgaccaggg cgaccttgtt ttccagtttc atatgatgct cctactgggg attactgttg
     4741 tgaagttgct cgctgcgatg atttacaggg caacgctgct ggctccaggc cctttgtcag
     4801 tctgggggac ataagggctg caaccgcgct gccccaagcg ccgggtgggc gctcatcgga
     4861 ttctagcacc gcgccgatgc gcgtcaaatg gcgacgtgtg gcgcgattct cccgaatcgc
     4921 gtgcgacgga aagtccgtta gcctaacgcc accgaagtgt gtataatgtt tacaaacatc
     4981 ataaacctta taagatacga ggccagcaat ggaagagcgc cagatcggaa caacagaact
     5041 gcggcaacgc ctgacagacg tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnatggaag agcgccagat cggaacaaca gaactgcggc aacgcctgac agacgtgatt
     5221 caggcggtga aggaagaaaa ggtggcctac gtggtagaga cgttcgggcg gccgcaggtc
     5281 gtgatcgttg acgccaatga gtaccagagc ctaaagacct accacgcgat gcgccaatcg
     5341 ttcttcgagc ggctactggt aactggcgaa gccaacgcca agatcaacgc cgacctgacc
     5401 gaggagcaac tcctggcgct cattgatgcg gcgcgcgatg aagtttatga tgagggctag
     5461 gagcggcggt tatgcttcgt gtcgtcatcg ataccagcac ccttgtcagc tttgctttga
     5521 cgtccggcga gatcaccagc caaatcattc gcgcctggca ggcagaggag ttcgttctgc
     5581 tgacgacagc cgccatgcga aacgagttgc ggcgcgtgct tgatcggccg cgcatccgga
     5641 cgcgagccga agcacccctg acctggattg ccgacgaatt ggaacatcac tcatggcata
     5701 tttcgggcca gctgaccgtc acgggcgtgg gccgcgatcc taaagatgac gtcttcctgg
     5761 cctgtgccgt cgaaggacag gccgattatc ttgtcagtag cgatcgcgat ttactggaca
     5821 tgcaccacta tcaggatatc tgcattgtca atccggggca gttcctcgtc ggcctgcaaa
     5881 tgaatcgtct gactgtcgag cagatcgtgg cccgcttcag ccctgcggct ctggaagccg
     5941 ttcaatcgac tttgtgcctc aatcgtgatg cagccgtgaa agtggctcag gctatgggaa
     6001 ggtaatgctg cccgccctcc tcgaccacct gcgcttcaat cgcgacttca tggccaatgt
     6061 cgtcgcctgg gagcgcattc cgccgcggcc ggcgcgcacc gccccgctgc cggactggct
     6121 cgacccacgg ctggcggcgg cgctgcgggg gcgaggcatc gaccaactct tcagccacca
     6181 ggccgcggcc attgaggccg tccgccgcgg cgagaacgtc atcgtcagca cagccacggc
     6241 cagcggcaaa tccctggcct acaccctgcc catcttgcaa cgcctgctgg agcgccccga
     6301 cgcccgcgcc ctctatctct tccccaccaa ggcgctggcc cacgaccagg aggcggaaac
     6361 agcttcgctg gtagccgccg cccgcctgc
//
LOCUS       DAOSMQ010000137         6365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12324_length_6365_cov_4.674485, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000137 DAOSMQ010000000
VERSION     DAOSMQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12324_length_6365_cov_4.674485"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK829_06035"
     CDS             <1..485
                     /locus_tag="PK829_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU40809.1"
                     /translation="PIFARRGVDLRRHLPNDLPSLYVEPNVISRVLLNLFDNALKYSH
                     DGQLVTVEGRAIDNGEVLISVSDQGAGIPKEYREIVFEKFRRIKNDSSSKGLGLGLAF
                     CRLAVEAHGGRIWIDDAPGGGARFNLTLPEAPRENASGPAEDSDSDGHWPDEAAGAIP
                     "
     gene            482..1720
                     /locus_tag="PK829_06040"
     CDS             482..1720
                     /locus_tag="PK829_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOU40810.1"
                     /translation="MSDRAYYADAYTTRFTAEVIEVTEVEGRPAVRLSSTYFYPTSGG
                     QPHDTGRLQQSHRTARVLEVVVREADDAVLHLLDGPLRPGEATAEIDWPRRFDHMQHH
                     TGQHILSQAFIRAAEAETSGFHLSGQSVTIDLDRPDLSPDEIRAAEIMANEVIEQNRP
                     VRARQVSLAEAATLPLRKRPPGRDGRLRLVDIDGFDLTACGGTHVAGTAEVGLVKVVK
                     TERRGGATRVEFLCGRRATADYRLKHDVTQQLSTALTTGVSDLAPALARLQEEARQLR
                     LALKRERGARLALEAGQLLDAAETIGGARLVCRVFAGREADEVRQLATAITAGGAVAL
                     LGLAGERAQLVFSRPAEGPTFAHMGELIRPALAALGEARGGGGAVSAQGGGPPASEAT
                     VGAALAAAVARLRAATNAVE"
     gene            1762..2475
                     /locus_tag="PK829_06045"
     CDS             1762..2475
                     /locus_tag="PK829_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU40811.1"
                     /translation="MAARDSIAAQALQGVPFFANLQEDHASELAGKLVLRRFGPNQVI
                     FHLGDPGGLLYLISRGKVKISHTTPDGQEVVLAILGTGDFFGELALLDDSPRSATAVA
                     LEPTETWTLHRAEFMQYLSDNPNFALHVLKTLARHIRRLNTQLADIFFLDLPGRLARV
                     LLNLSEQYGRKTQEGTQIELSLTQTDLAEMTGATRVSINKALGRFRRAGWIRVNGRRV
                     TVLDEAALRSLIEVSGGDL"
     gene            2650..>6365
                     /locus_tag="PK829_06050"
     CDS             2650..>6365
                     /locus_tag="PK829_06050"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOU40812.1"
                     /translation="MSVTGLDNDPELVPPAASSPAVPRARARGEALVAVALVAILLLG
                     AYFRFVGFNWDSDSHLHPDERFLTMVGSAISGVDNPLDYLRTSISTLNPYNFGQSLYV
                     YGNFPMTATRYVGEWATGLCDAAASRTGTLPGWCSHTYSGYDGIQLVGRFLSGLVDLI
                     SVFFTFLIARRLYDWRAGLFAALLLALAVMPIQQSHFFTMDNWATAFTTLGVYAAVRA
                     ATVGDAAPRWRLRWWALFGLALGLAAASRVNTAPLAVLIAVSAVIFLARREASVVPDA
                     DAPAGRLSWLARATRTRDLERAIVGIALAAAVSILTFRVAQPYAFTDAQMVRDQAIAT
                     TGQEPSAFIVAIKSVIGLNPAFLSNMAEIQRLQAPEASFPPATQWVDRPAILFPLSNM
                     LLYGMGITAGLFAWFAVFWAAWRSLGSARRAAAGGADWPLPEWMLHAIPLAWTLLYFL
                     FMGTRWVKSVRYFLPIYPTLFVLAGWAIVQLWLWARRPVSPTTSAARGQLPPRWRAAA
                     VAALAALIVLPSLLWAVVYTNIYREPMTRIAASEWIYDNVPSGATLLYDTADGRAGEL
                     NLPLKGMDFQLDGLPLFLDFTMPEDGTIRALRLNYLSDPDFDASAAGVVPDADTETLR
                     VILGEGTPVDQPLQLDGERRAAVIPLAATPAAAGAPIHLTLQAGPGGPIRAGTSLLVN
                     EHWDDLLPVGLDGRNAYGAYYTEVTGGQRPVTNPDSPEKREEVIAWLDEADYIMISSQ
                     RAMWSLPRMALTYPLMLRYYEGLFSGELGFDLVAQFHENTHLGPLYISDTSAQVSWGQ
                     PPTVGWPPPSELAAEEAFSVYDHPPVWIFAKNDRYSSENTRRILESVDLSQVRVMNPL
                     EATRATNGMMLSAADRATQQAGGTFSEVFAIDGLLSRNPWLAAVAWALAVIVIGWIAF
                     PLAYTALGGLPDRGYGLSRVLGLLVVSYLPWLAASVNWLPHTRGTLLLALLALFAISA
                     SLFARRRREMLAFVRRRWRYLLGVELLGLALFALMVAIRLGNPDVWDVIWGGEKPMDL
                     SYLTAVMRSTTFPPYDPWFAGGYLNYYYYGFVFVGALAKLLGVMPAVAYNLILPMLFS
                     MIGVAVFSLAHGLVVAVGPRPRVARFSRIAFLRPPVIAGLVAVTLALLLGNLAEPTVV
                     ADAWYKAGTLSLEELPVIGGAARTLDGGLKILSGQPAPLYPGDWFWTASRAINANPGE
                     VQPITEFPFFTFLYGDLHAHMIAMPLSMLALGWA"
BASE COUNT          977 a         2181 c         2148 g         1059 t
ORIGIN      
        1 agccgatctt cgcgcggcgc ggcgtagacc tgcggcgcca tctgcccaac gacctgccgt
       61 cgctctacgt cgagccgaat gtcatcagcc gggtgctgtt gaacctgttc gacaacgctc
      121 tgaagtatag ccacgacggc cagttggtga ctgttgaggg ccgggccatt gacaacggcg
      181 aggtgctcat cagcgtcagc gaccagggcg cgggcatccc caaggagtac cgcgaaatcg
      241 tcttcgagaa gttccggcgc atcaaaaacg actcctcgtc gaaggggctg gggctggggc
      301 tggccttctg ccggttggct gtagaagccc acggcggccg catctggatc gacgacgcgc
      361 ccggcggcgg cgcgcgcttc aacctgacgc tgcccgaagc gccgcgcgag aacgcctccg
      421 gcccagccga ggacagcgac agcgacggcc attggccaga tgaagccgcc ggcgcaatcc
      481 catgagcgac cgcgcctact acgccgacgc ctacaccacc cgcttcaccg ccgaagtcat
      541 cgaagtgact gaggtggagg ggcggccggc ggtgcggttg tcgtccacct acttctaccc
      601 cacctctggc ggccaacccc acgacaccgg ccgcttgcaa cagagccacc ggacggcccg
      661 ggtgctggaa gtcgtcgtgc gcgaggcgga tgacgccgtg ctgcacctcc tcgacgggcc
      721 gctgcgcccg ggcgaggcca cggccgagat cgactggccg cgccgtttcg accacatgca
      781 acaccacacc ggccagcaca tcctgtcgca ggcctttatc cgcgcggccg aggccgagac
      841 gagcggcttc catctcagcg gtcagagcgt gaccatcgac ctggatcggc ccgacctttc
      901 gcccgacgag attcgcgccg ccgagatcat ggccaacgag gtcatcgagc agaaccggcc
      961 ggtgcgggcg cgacaggtgt cgctggccga ggcggcgacg ctgccgctgc gcaaacgccc
     1021 gccggggcgc gatgggcggc tgcggctggt ggacatcgac ggtttcgacc tgaccgcctg
     1081 cggtggcacg cacgtggccg gtacggccga ggtgggcctg gtcaaggtcg ttaagacgga
     1141 acggcgtggc ggggccacgc gggtagagtt cctctgcggc cgtcgggcca cggccgacta
     1201 tcgcctaaaa cacgacgtga cccagcagtt gagcacggcc ctaaccacgg gcgtcagcga
     1261 cctcgcgccg gcgctggccc gcttacagga ggaagcgcgg caattgcgcc tggccctcaa
     1321 gcgggagcgc ggcgcgcggt tggccctgga ggcggggcag ctgctcgacg ccgccgagac
     1381 cattggcggg gcgcgcctgg tctgtcgcgt cttcgccggg cgcgaggcag acgaggtgcg
     1441 ccaactggcc accgcgatca ccgccggggg cgcggtggcg ctgctgggtc tggccggcga
     1501 gcgagcgcaa ctggtgttca gccgcccggc cgaggggcca acgtttgccc acatgggcga
     1561 gctgatccgg ccggcgctgg ccgcgctggg cgaggcgcgc ggcggcggcg gggcggtgtc
     1621 ggcccagggc ggcgggccgc cggccagcga ggcgaccgtc ggcgcggcgc tggccgccgc
     1681 tgtggcccgc ttgcgggccg cgacgaacgc ggtagaatga agagactgta gaggaaaccg
     1741 gagaaaaggg aggatgggag tatggcggca cgcgatagta tcgccgcgca agccttgcaa
     1801 ggcgtgccct tctttgccaa tttacaggaa gaccacgcca gtgaattggc cggcaagctt
     1861 gtcttgcggc gttttggccc caatcaagtc atcttccacc tgggcgatcc cggcggtctg
     1921 ctttacttga tcagccgcgg caaggtcaag atcagccata ctacacccga cggccaggag
     1981 gtcgtgctgg ccatcctggg cacgggcgac ttcttcggcg agctggccct gctagacgac
     2041 tcgccacgct cggccacggc cgtggccctg gagccaacgg aaacctggac gctccaccgg
     2101 gccgagttta tgcagtacct gagcgacaac cccaactttg ccctgcacgt cctgaagacc
     2161 ctggcccgcc acatccgccg cctgaatacc caactggccg acatcttctt tctcgacctg
     2221 cccggccggc tggcgcgcgt gctgctgaat ctgtccgagc agtatgggcg caagacgcag
     2281 gaaggcaccc agatcgagct atccctcacc cagaccgacc tggccgagat gaccggggcg
     2341 acgcgcgtca gcatcaacaa ggcgctgggg cgcttccggc gggccggctg gattcgcgtc
     2401 aacggccggc gcgtcaccgt ccttgatgag gctgctctga ggagcttgat cgaagtatcg
     2461 ggcggggacc tgtagctcac ggcctctttt cgtccgcttt ccagcttaga aaatcgacat
     2521 ttcagtgagc agagaaagag catgttgtgt ataaacatgt gattgcgcgt tgagtgggtg
     2581 acggtttggt gacaatcacc gccaccgcca ccgccacaat acagaaaaga caacatcgat
     2641 agcgatcgta tgagcgtaac cggcctggat aacgaccccg aacttgtccc acccgctgcg
     2701 agtagtcccg ccgtcccccg cgcccgcgcg cgaggagagg cgcttgtcgc cgtcgccctt
     2761 gtcgccatcc tgttgctggg ggcgtatttc cgctttgtcg gcttcaactg ggatagcgac
     2821 agccatctgc accccgacga gcgcttcctg acgatggtcg gcagcgccat cagtggcgtt
     2881 gacaacccgc tcgactacct gcgcacgtcg atctccacgc tgaaccccta caacttcggc
     2941 cagtcgctct acgtctacgg caacttcccc atgacggcga cgcgctatgt gggcgagtgg
     3001 gccacgggac tgtgcgacgc cgccgccagc cgcacgggga cgctaccggg ttggtgcagc
     3061 cacacttaca gcggctatga cggcatccag ttggtcggcc gcttcctctc aggcctcgtc
     3121 gatctcatca gcgtcttctt caccttcctg atcgcccggc ggctgtacga ctggcgggcg
     3181 gggctgttcg ccgccctgct gctggcgctg gcggtcatgc ccatccagca aagccacttc
     3241 ttcacgatgg acaattgggc gacggcgttc acgacgctgg gcgtctacgc cgccgtgcgg
     3301 gcggcgacgg tgggcgacgc cgcgccgcgc tggcggctgc gctggtgggc gctgttcggg
     3361 ttggcgctgg ggctggccgc cgcctcgcgg gtcaacaccg cgccgctggc ggtactcatc
     3421 gccgtcagcg cggtcatctt cctggcccgg cgggaggcga gcgtcgtccc cgacgccgac
     3481 gcgccggccg gtcgcctttc ctggctggcg cgggcgacgc gcacgcgtga cctggagcgg
     3541 gcgattgtcg gcattgccct ggccgcggcc gtgtccatcc tgaccttccg cgtggcccag
     3601 ccctatgcct tcaccgacgc gcagatggtg cgcgaccagg ccatcgccac gacaggccag
     3661 gagccgagcg ccttcattgt cgccatcaag tcggtcatcg gcctgaaccc cgctttcctg
     3721 agcaacatgg ccgagattca gcgtctgcaa gcgccggagg cctcctttcc cccggcgacg
     3781 caatgggtcg atcggccggc catcctcttc ccgctcagca acatgctgct ctacggcatg
     3841 ggcatcaccg ccgggctatt cgcctggttc gcggtcttct gggcggcgtg gcggtctctg
     3901 ggcagcgcgc gccgcgccgc ggccggcggc gcggactggc cactgccaga gtggatgctc
     3961 catgccatcc cgctggcgtg gacgctgctc tacttcctgt tcatgggcac gcgctgggtg
     4021 aagtcggtgc gctacttcct gccaatctac ccgacgctgt tcgtgctggc cggctgggcc
     4081 attgtccaac tgtggctatg ggccagacgg ccggtgtcgc caacgacttc ggcggccagg
     4141 ggccaactcc cgccgcgctg gcgcgcggcg gccgtggccg cgttggccgc gctgatcgtc
     4201 ctgccgagcc tgctgtgggc ggtggtttat accaacatct accgcgagcc gatgacgcgc
     4261 atcgccgcct cggagtggat ctacgacaat gtgcccagcg gggcgacgct gctctacgac
     4321 acggccgacg gccgggccgg ggagctaaac ctgccgctga agggcatgga cttccaactc
     4381 gacggcctgc cgctgtttct cgacttcacc atgcccgaag acggcacgat ccgcgccctg
     4441 cggctcaact acctgagcga tccggacttc gacgcgagcg cggcgggcgt cgtccccgac
     4501 gccgataccg aaaccttgcg cgtcatcctg ggcgagggca cgccggtgga tcagccgctg
     4561 caactggatg gcgagcggcg ggcggcggtc atcccgctgg cggccacgcc ggcggcggct
     4621 ggcgcgccca tccacctgac cctgcaggcc gggccgggcg gcccgattcg cgccgggacg
     4681 agtctgctgg tcaacgagca ttgggatgat ctgctgccgg tggggctgga cgggcgcaac
     4741 gcctatggcg cgtactacac tgaagtgacc ggcggccagc ggccggtgac caaccccgac
     4801 agcccggaga agcgcgaaga ggtcatcgcc tggctggacg aggccgacta catcatgatc
     4861 agcagccagc gggccatgtg gagcctaccg cggatggcgc tgacttaccc gctgatgctg
     4921 cgctactacg aagggctgtt cagtggtgag ttgggcttcg acctggtggc ccaattccac
     4981 gaaaacacac acctggggcc gctctacatc agcgacacgt cggcgcaggt gagttggggc
     5041 caaccgccta ccgtcggctg gccgccgcca agcgaactgg ccgccgaaga ggccttcagc
     5101 gtctacgacc acccgccggt gtggatcttc gccaagaacg accgctacag cagcgagaac
     5161 acgcgccgca tcctggagag cgttgacctg agccaggtgc gggtgatgaa cccactggag
     5221 gcgacgcggg ccaccaacgg catgatgcta tcggccgccg accgggcgac gcagcaggcc
     5281 ggcggcacgt tcagcgaggt ctttgccatc gatggcctgc tgtcgcgcaa tccgtggctg
     5341 gcggccgtcg cctgggcgct ggcggtcatc gtcatcggct ggatcgcctt tccgttggcc
     5401 tacaccgctt tgggcggcct gcccgatcgc ggctatggcc tgtcgcgggt gctggggctg
     5461 ctggtcgtgt cgtacttgcc ctggctggcg gcgagtgtca actggctgcc gcacacgcgc
     5521 ggcacactgc tgctggccct cctggctctc ttcgccatca gcgccagcct gttcgcgcgg
     5581 cggcgacggg agatgctggc cttcgtgcgc cggcggtggc gctacctcct cggcgtggaa
     5641 ttactcgggc tggccctctt cgccctcatg gtcgccatcc gcctgggcaa cccggacgtg
     5701 tgggatgtca tctggggcgg cgagaaaccc atggacctgt cgtacctgac ggcggtgatg
     5761 cgctcgacga ccttcccgcc ctatgacccg tggttcgccg gcggctacct gaactactac
     5821 tactacggct tcgtcttcgt cggggcgctg gccaagctgc tgggcgtgat gccggccgtg
     5881 gcctacaacc ttattctgcc aatgctgttc agcatgatcg gcgtggccgt gttcagcctg
     5941 gcacatgggc tggtagtcgc cgtagggccg cggccgcgcg tggcgcgatt ctcccgaatc
     6001 gcgtttctta ggccgcccgt cattgccggg ctggtggccg tgaccctggc gctgctgctg
     6061 ggcaacctgg ccgagccgac ggtggtagcc gacgcctggt ataaggccgg cacgctgtcg
     6121 ctggaggaat tgccggtcat cggcggcgcg gcgcggacgc tcgacggcgg cctgaagatc
     6181 ctcagcggcc agcccgcgcc cctctacccc ggcgactggt tctggacggc ctcgcgggcc
     6241 atcaacgcca acccgggcga ggtgcagccc atcaccgagt tccccttctt caccttcctc
     6301 tacggcgacc tgcacgccca catgatcgcc atgccgctga gcatgctggc gctgggctgg
     6361 gccat
//
LOCUS       DAOSMQ010000138         6353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12355_length_6353_cov_4.223245, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000138 DAOSMQ010000000
VERSION     DAOSMQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12355_length_6353_cov_4.223245"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="PK829_06055"
     CDS             <1..1495
                     /locus_tag="PK829_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40813.1"
                     /translation="SLAELKDLAFTVGIDPESVAGDTRPEFGLGLARAAWCRGCLAAL
                     LAEAARQVPAVDWALPALPAPPETGCEDDPLPRPSVFRDSRQVATFVLIPLLAAALVA
                     AGVWWTRQPARLDADFNVAVAAIDTTGGSAAQNEQAAALLQQVVVDEMERQLNAVLDE
                     EIVVSDARMPVVADEVDAERLARRVNAQLVIFGTARIDAAGRVVYSPHFYIYFDPARQ
                     DVAELTGDYPLDVKLTFKLADLMTDRLPPARTAAAAALLTRFGQALVYLKAHDLDNAY
                     WQIVNAVSRTDAYAVTYEPFKGREVIYLFAGHIARLQASAAGREATARESLLQKAEAF
                     NAQALDINPNYGRALIGRANLAYDRGDLLAAKQDYEAAAALINQPPTARIGAKAAMGL
                     GNVYLSQLNGPANGTVCVDERRRLADEGLAYYGRVLAEYDAHRSEALLADIAGQAHLY
                     RGEIYYICRQNDEAAKEFRAARAWKGLPPALRSNAEELLKAVKARPD"
     gene            1540..2616
                     /locus_tag="PK829_06060"
     CDS             1540..2616
                     /locus_tag="PK829_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU40814.1"
                     /translation="MRRILFGLLLALALAGCRPENEGATGADVASNGATSVAASVATA
                     EGPAATAAPGATPVAGRVFWDTATPTPPASTFWPTASPTPTPTGTATAPPEVIAEVVP
                     LPVICQPPAGWVAQPIRPGDTVGALAACVGAPVGEVLAANCLSPDGLIFAGQELWLPA
                     ACAPPEDSGISIAPLQPTPPPELPPGTPPASPSPEPSIVSPMAIQPGNMILTVVPDTA
                     RPGETVMLSQTGFPANSAVKLTFRDGESRRCDTVTVSPTADASGRLSFPLIVPQIFPN
                     GVVLIAAEADAGGVRADGNLIVSGSSAATWCAIPSPAVATPSPTAMPSPTAMPSPTAM
                     PSPTATATDTPTLTPEPTSPASTG"
     gene            2678..4102
                     /locus_tag="PK829_06065"
     CDS             2678..4102
                     /locus_tag="PK829_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40815.1"
                     /translation="MTTDTNAPWPVDPWQGNDTGPVFGSGSQDDEPSPDVLPPADGPG
                     GFEQRAPQRLIIKTREGAFASYQDRLEEQLGEQLGSGWAELHERYPDLTIGRAFRGIE
                     GNDDSVPPEVLTYYEILLPPDQALEVGRAASILLERLSDQIEFTYIEAPPAPQPTCSG
                     VPRPYLNAAPRGMNVLPFQGSAADAWPVVVDLERAWDATSHPRVAHVGVLPGFAGPSP
                     ADGYARFVGEDLHATRSLSVLTAAENPVTHDCAGLLPANAPVFMASSLVWEPDGWVER
                     INSQIELVTKPGNGMQAGDILLIELQIPGRDRHGCVEVLAPIELEPANQAALATALSR
                     GIIVIEPAGNGGTNLTHLKLWDRCQAGSVRGVNALCVAACCAIAPEDIAPVRLLASNF
                     GSAVTFFSWGENIPTNVLPALELYGETSAASALIAGIAARVQSAAMTRGSGRLNIDQL
                     RAALQLGATLAPGQSIGVMPKLNP"
     gene            complement(4108..4536)
                     /locus_tag="PK829_06070"
     CDS             complement(4108..4536)
                     /locus_tag="PK829_06070"
                     /inference="COORDINATES: protein motif:HMM:NF039977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6069 family protein"
                     /protein_id="HOU40816.1"
                     /translation="MTTPVSPTGRRSLLTAALASVAAAVAANLVALLLVRAVVDLPDN
                     FPPLTAGSVAAFTLIGTGLGALVYAWLRRRSATPGRTYGRVAVVALILSIIPNFLAMA
                     NPAMFPFPGGNATAFLVLTLFHVIAAAVSVAVLLRLAGGR"
     gene            complement(4557..5426)
                     /locus_tag="PK829_06075"
     CDS             complement(4557..5426)
                     /locus_tag="PK829_06075"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU40817.1"
                     /translation="MSGTSLVTGSSGNVGREVVRALAARGAAVRAAIYSRADRPTLAG
                     VEPVRFDFADPKTFRDAFAGVDRLFLVRPPAIGNVERDIQPTIDYAVRAGVNHIVFLS
                     LLGAERNPLLPHARIEALLLASGVPWTLLRPGFFMQNLDTTHRADIVEHGDLFIPAGR
                     ARTAFIDTRDIGDVAARVLTEPGHEGVAYALTGSESLSYDDVAAIMTAELGRPITYSH
                     PSSLAFARRMRQRGHPWGYVAVMLGVYATTRRGTAAQVYPDAATLLGRPPRTLRQYVA
                     DYAALFAPALPAQ"
     gene            5645..>6353
                     /locus_tag="PK829_06080"
     CDS             5645..>6353
                     /locus_tag="PK829_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40818.1"
                     /translation="MRRYWSMIYIIAAIILTVVVGLLGFLPLVVRWLAVMGVFIGLLV
                     LIGREVTGQTVETTRRSRSLTRFTPGRADGVLIDLRNKISLSRLQLVLWTVVVLSAWV
                     TLALHRTIPVVAGRLPGPGSATVTTVAGLLAEDGATGQQATARATAVVAQLMGADAAA
                     NVDTASYSPLNIAFPQEVLLALGISVASLAGAGAIKSNRAADENGRATQIVENRVDRA
                     QEAAAHAGAQVTTLESTV"
BASE COUNT         1015 a         2197 c         2221 g          920 t
ORIGIN      
        1 cagcctggcc gagttgaaag acctggcctt cactgtcggc atcgacccgg agagcgtggc
       61 cggcgacacg cggccggaat tcggcctggg actggcccgc gcggcctggt gtcgcggctg
      121 tctggccgcc ctgctggccg aggccgcccg gcaagtgccc gccgtcgatt gggcgctgcc
      181 cgccctgccc gccccgccgg agaccggctg cgaggacgac ccgctgcccc ggccgtcggt
      241 cttccgcgac tcgcggcagg tggccacttt tgtactcatc cccctcctgg ccgccgcgct
      301 ggtggccgcc ggcgtctggt ggacgcgcca gccggcgcgc ctggacgccg acttcaacgt
      361 cgccgtggcc gccatcgaca cgaccggcgg cagcgccgcg caaaacgagc aggcggcggc
      421 gcttctgcaa caggtggtcg tcgatgagat ggaacggcag ctcaacgccg tcctcgatga
      481 ggaaatcgtt gtcagtgacg cgcgcatgcc cgtcgtcgcg gacgaggtcg acgccgagcg
      541 gctggccagg cgcgtcaacg cccagctggt catctttggc acggccagga tcgacgccgc
      601 cggccgggtg gtgtactcgc cccacttcta catctacttc gacccggcgc ggcaggacgt
      661 ggccgagttg acgggcgact acccgctgga tgtcaagctg acgttcaagc tggccgatct
      721 gatgaccgac aggctcccgc ccgcgcgcac ggccgccgcc gccgcgctgc tgacgcgctt
      781 cggccaggcg ctggtctatc tgaaggccca cgacctcgac aacgcctact ggcaaatcgt
      841 caacgccgtc agccgaaccg acgcctatgc ggtcacctac gagccattca aagggcgcga
      901 ggtgatctac ctgtttgccg ggcacatcgc ccgcttgcag gcgagcgcgg cgggccgcga
      961 ggcgacggcg cgcgaaagcc tgttgcaaaa ggccgaggct ttcaacgccc aggcgctgga
     1021 catcaacccc aactacggcc gcgccctcat cggccgggcc aatctggcct acgaccgcgg
     1081 cgaccttctg gcggccaagc aggactatga ggccgcggcg gcgctgatca accagccgcc
     1141 caccgcgcgg atcggcgcca aggccgccat ggggctgggc aacgtctacc tgagccaact
     1201 caacggcccg gccaacggca cggtgtgcgt cgacgagcgc cggcggttgg ccgacgaggg
     1261 gctggcctac tatggccggg tgctggccga gtatgacgcc caccggtcgg aggcgttgct
     1321 ggccgacata gccggccagg cccacctgta ccggggcgaa atctactaca tatgccgcca
     1381 gaacgacgag gccgcgaagg agtttcgcgc cgcgcgggcg tggaagggcc tgccgccggc
     1441 gctgcgatcc aacgcggagg aactgctgaa ggcggtgaag gcccggccgg attagggcgt
     1501 ggccgtggcc gtcgtgggct gtgacaggag ggtaggttca tgcgcaggat actgttcggg
     1561 ttgttgctgg cgctggccct ggccggctgc cggccagaga atgagggcgc gacaggcgca
     1621 gacgtggcct cgaacggagc cacgagcgtg gccgcgagcg tggccacggc agaggggccg
     1681 gcggcgacgg ccgcgcccgg cgcaacgccg gtggccggca gggtcttttg ggacacggcc
     1741 acgccgacgc cgcccgcgag cacgttttgg cccaccgcct cacccacgcc gacgccgacc
     1801 ggcacggcca cggcgccccc ggaggtgatt gccgaggtcg tgccgttgcc cgttatctgc
     1861 cagccgccgg ccggctgggt ggcgcaaccc atccggcccg gcgacacggt gggggcgctg
     1921 gcggcctgtg tcggcgcgcc ggtgggcgag gtgctggccg ccaactgcct gtcgccggac
     1981 gggctgatct tcgccgggca ggagttgtgg ctgccggcgg cctgtgcgcc gccggaggat
     2041 agcggcattt cgatcgcgcc gctgcagcca acgccgcccc cggagctgcc ccccggcacg
     2101 ccgccagctt cccctagccc ggaaccatcg atcgtctctc ccatggcgat ccaacccggg
     2161 aacatgatat tgaccgtcgt tcccgacacc gcccgccccg gcgaaacggt tatgctcagt
     2221 cagacgggct tcccggctaa cagtgcggta aagctgacct ttcgcgatgg tgaatcccgc
     2281 cgctgcgaca cggtcaccgt atccccgacg gccgatgcca gcggtcgatt gagctttcca
     2341 ctgatcgtgc cgcagatctt tccgaatggc gtcgtcttga tcgccgccga ggcggacgct
     2401 ggcggcgtcc gggccgatgg caacctgata gtcagcggtt ccagcgcggc gacgtggtgc
     2461 gccatcccgt cgccagctgt ggccacgcca tcgcccacgg ccatgccatc acccacggcg
     2521 atgccatcgc ccacggccat gccatcgccc acggctacgg cgacagacac acccacgctg
     2581 acgcctgagc caactagccc ggccagcaca gggtgaggat gatcccggcc agcgcgaaat
     2641 ggagaacaag cgataagaaa tacgcaggag gacgatgatg accacagata cgaatgcgcc
     2701 ctggccggtt gacccctggc agggcaacga cactgggcca gtgttcgggt cgggtagcca
     2761 ggacgacgag ccgtcgccag acgttcttcc gccggccgac gggccgggcg gcttcgagca
     2821 gcgcgccccc cagcggctta tcatcaagac ccgcgagggc gcgtttgcga gctatcagga
     2881 ccggctggaa gagcaactgg gcgagcaact ggggtctggc tgggcggaac tgcatgagcg
     2941 ctacccagac ctgaccatcg gccgcgcctt ccggggcatc gagggcaacg acgacagcgt
     3001 gccgccggag gtattgacct actatgaaat cctgttgccg cccgaccagg cgctggaggt
     3061 gggcagggcc gcctccatcc tcctggagag gctctccgac caaatcgaat tcacctacat
     3121 cgaagccccg cccgcgcccc aaccgacgtg cagcggggtg ccgcgtcctt acctcaacgc
     3181 tgcgccacgc gggatgaatg tattgccctt tcagggcagc gccgccgacg cctggccggt
     3241 cgtcgtcgac ctggagcgag cctgggacgc caccagtcat ccgcgagtgg cccatgtcgg
     3301 cgtcctgcct gggttcgccg ggccatctcc ggcggatggc tacgcccgtt ttgtgggcga
     3361 agaccttcat gccacgcgct cgctgagcgt cctcacggcc gcggaaaatc cggtcacgca
     3421 cgactgcgcc ggcctgttgc cggcgaacgc gccggtgttc atggcctcat ccctggtttg
     3481 ggaaccagac ggctgggtgg aacgcatcaa ctcacagatc gagttggtca cgaagccggg
     3541 taatgggatg caagccggtg acattctgct aatcgagttg caaatccccg gccgggacag
     3601 gcatggttgc gtagaggtgc ttgcgcccat cgagttggag ccggccaacc aggcggcatt
     3661 ggctaccgcc ctgtcacggg gcatcatcgt cattgagccg gccggcaacg gcggcacaaa
     3721 cctgacgcac ctgaagctgt gggacagatg tcaggctggc agtgtgcggg gcgtcaatgc
     3781 tctttgcgtg gccgcgtgct gcgccatcgc tcccgaggac attgcgcccg tgcggctgct
     3841 ggcgtcgaat tttggcagcg cggtcacctt cttcagctgg ggcgaaaata tacccacaaa
     3901 tgtgctcccc gcgctagagt tgtacggcga aacctcggcc gcgtcggccc tgatcgccgg
     3961 tatagcggcc cgcgtccaga gcgcggccat gacgcggggg agcggccggt tgaacatcga
     4021 ccagctgcgc gcggcgctgc agctgggggc caccctcgcc ccggggcaaa gcattggcgt
     4081 catgcccaag ctgaacccgt agcgccccta ccgccccccc gccaaccgca acaacaccgc
     4141 cacactgacg gccgcggcaa tgacgtggaa cagcgtcagc accaggaagg ccgtggcgtt
     4201 gccgccggga aaggggaaca tggccgggtt ggccatggcc aggaagttgg gaatgatcga
     4261 gaggatcagc gcgacgacgg cgacgcggcc gtaggttcgc cccggcgtgg ccgagcggcg
     4321 gcgcagccag gcatagacca gcgcgcccag ccccgtgccg atcagcgtga aggcggccac
     4381 cgacccggcc gtcagcggcg ggaagttgtc cggcagatcg accacggcgc gcaccagcaa
     4441 cagggccaca aggttggcgg cgacggcggc ggccacggaa gccagggcgg cggtcaacag
     4501 gctgcggcgg ccggttggcg aaacaggtgt ggtcatggat gtctcctcaa gaataactat
     4561 tgtgccggca gcgccggcgc gaacagggcg gcgtagtcgg ccacgtactg gcgcagcgtg
     4621 cgcggcgggc ggccgagcag cgtcgccgcg tcgggataga cctgggcggc cgtgccccgc
     4681 cgcgtcgtgg catagacgcc cagcatgacg gcgacgtagc cccacgggtg gccgcgctgc
     4741 cgcatgcgcc gggcgaaggc cagcgacgac gggtggctat aggtgatcgg ccggcccaac
     4801 tccgcggtca tgatggcggc cacgtcgtcg tagctgagcg actcgctgcc cgtcagcgca
     4861 taggccacgc cctcgtggcc cggctcggtc agcacgcgcg cggccacgtc gccgatgtcg
     4921 cgcgtgtcga tgaaggctgt gcgggcgcgg ccggcgggga tgaacaggtc gccatgctca
     4981 acgatgtcgg cccggtgggt cgtgtccagg ttctgcatga aaaagcccgg ccgcagcagc
     5041 gtccagggca cgccggaagc cagcagcagc gcctcgatgc gcgcgtgggg cagcaggggg
     5101 ttgcgctccg cccccaacag cgataggaag acgatgtggt tgacgccggc gcggacggcg
     5161 tagtcgatgg tcggctggat atcgcgctcg acgttgccga tggccggcgg tcgcaccaga
     5221 aacagccggt cgacgccggc gaaggcgtcg cgaaacgtct tgggatcggc aaagtcaaag
     5281 cgcaccggct cgacgccggc cagagtgggc ctgtcggcgc gcgagtagat cgccgcgcgc
     5341 accgccgcgc cccgcgccgc cagcgcccgc accacctcgc ggccaacgtt gccgctagat
     5401 cccgtcacca aagacgtgcc gctcatgggg gcgatcatag cggctggcgt ggggcgcgca
     5461 agggagacgg ccgacgacag accacagacc gctgccggct ggtcgtcgcc ggccgtccct
     5521 cctctggctg cgcccgtggt ctggcgtcct cttcggcccg ccccgcactc cccattgcct
     5581 cgcgccgcct gcgcccctat aatcctcctc tctgacaggt gggtgttcgc taggaggtat
     5641 tatgatgcgt cgctattggt ctatgatcta catcatcgcc gccatcatcc tgaccgtcgt
     5701 ggtcggtttg ctcggtttcc tgccgctggt ggtgcgctgg ctggccgtca tgggcgtctt
     5761 catcggcctg ctggtgctca tcggccgcga agtgaccggc cagacggtgg agacgacgcg
     5821 ccgcagccgt tcgctgacgc gcttcacgcc cggccgcgcc gacggcgtgc tcatcgacct
     5881 gcgcaacaag atcagcctgt cgcggctgca actggtgctg tggacggtgg tggtgctgtc
     5941 ggcctgggtg acgctggcgc tgcaccgcac catcccggta gtggccgggc ggctgccggg
     6001 gccgggcagc gccacggtga cgacggtggc cgggctgctg gccgaggacg gcgcgaccgg
     6061 ccagcaggca acggcgcggg cgacggccgt cgtggcccag ctgatgggcg cggacgctgc
     6121 cgccaacgtg gacacggcca gctattcgcc gctcaatatc gccttcccgc aagaagtgct
     6181 gctggctctg ggcatcagcg tcgcctcgct ggccggggcg ggggccatca agagcaaccg
     6241 cgccgccgac gaaaacggcc gggcgaccca gatcgtcgag aaccgcgtcg accgcgccca
     6301 ggaggccgcc gcccacgccg gggcgcaggt cacgaccctg gaatcgaccg tcg
//
LOCUS       DAOSMQ010000139         6292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12501_length_6292_cov_3.905564, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000139 DAOSMQ010000000
VERSION     DAOSMQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12501_length_6292_cov_3.905564"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PK829_06085"
     CDS             <1..1071
                     /locus_tag="PK829_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOU40819.1"
                     /translation="DVNFSATSASMGAAVMRTFRAQAWAIWLLGGGVLVLATRNPLYL
                     VLLLAISRIVQATCAAGSIGPRLQYGRIAALILLFSTLFNLLTAHIGETVLLTLPAGW
                     WLIGGPITLEAAVYGFITGLGLVTLLSFFVAFNAIVPTGELIGLAPAALYELGLVLLL
                     AITYVPETGRQLQRIREAQAIRGHRLRSLRDWQPIVIPLLVGGLERAMNLSETMVARG
                     FGSVAHVATPWRVRLGLLAGLVLTLAGALRLSWAGADGWALIGAGAVAIGLAYRALSR
                     GVRRTRYRPRPWTPADTAVVIGALLPLLLLLPLPALGGAGRGYEPYPRLALPAFAPWA
                     GTLLLGLAAPAAWQAVPAPEDT"
     gene            1068..2738
                     /locus_tag="PK829_06090"
     CDS             1068..2738
                     /locus_tag="PK829_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU40820.1"
                     /translation="MTADGIIAIRRLTYHYPQRPTPALRDVTWDVADGEFVLVAGASG
                     SGKSTLLRCLNGLVPHFSGGVLAGQMAVAGIDVIAAGPRVMSRHVGFVAQDPEAQAVL
                     DRVEGEIAFALENAAVPPAEMHRRVEQALDLVELTPLRDRPLDTLSGGERQRLAIAAA
                     LALQPRVLVLDEPTSQLDPQSAEEVLRALVRLKAGLGLTIILAEHRLERIARYADRLT
                     FLEDGRVVLDGPVRTTLPLIPGAPPVVALGRALGWQPAPLTVKEARPLAATLPVAAFP
                     VPQATPVPEKPIAPRLQVEGLRFSYNGQETLRGIDLSVAPGEVVALVGRNGAGKSTLL
                     KCIVGLLRGQAGEVRVNGRSTLGRHVADICREIGYLPQNADDLLFADTVADEMRVTLR
                     NHGLAATAVEQAAWLARLGLTELARAYPRDLSAGQRQRVALGAVTATQPRLLLLDEPT
                     RGLDWAAKTALVALWRGWLAEGAAMLLVTHDVELAATFAQQTVILSRGEVIAAGPTRD
                     VLAGAPHFAPQMARLFPGRGWLTVEDALRGLGRPASGANEWEDKQCRD"
     gene            2726..3289
                     /locus_tag="PK829_06095"
     CDS             2726..3289
                     /locus_tag="PK829_06095"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOU40821.1"
                     /translation="MPRLTKIYTRGGDAGQTSLSGGQRVAKNSLRVAAYGTVDELNSY
                     LGVALAAGLTPRLAGVVAEIQNELFHLGSDLSFLEADKADRPLPQIEPRHVARLEALI
                     DALTEIVGPLQNFILPGGAPTSAHLHVARAVCRRAERDVIALAREEAVGAQVMPYLNR
                     LSDALFVMARYENHARGIAEPLWDSHA"
     gene            3394..3558
                     /locus_tag="PK829_06100"
     CDS             3394..3558
                     /locus_tag="PK829_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40822.1"
                     /translation="MLHNLAVAAIVVVILWVIILGLYLMVTRRQPDVAAQMKALEERL
                     ERAEREAKQR"
     gene            3567..4241
                     /locus_tag="PK829_06105"
     CDS             3567..4241
                     /locus_tag="PK829_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40823.1"
                     /translation="MSTTLRRFLVTFVVLLALLPLMACLGAAETEPDACDGPDFLFAD
                     NFNGELDCGWATYDRGGGTATIENAAMQVTVGQPGQLWWTNPGKTFDDVVITAEARQV
                     DGPNDNAYGLICRYQDESNFYVFLVSGDGYYAIGKYQSGSDSVSYLTPNAQFQSSDAI
                     NVGVASNELRVSCIGSELSLEVNGIPLMTVSDPTFVNGDIGLAASTLQPGTTIVEFDN
                     VRVTQP"
     gene            4219..4908
                     /locus_tag="PK829_06110"
     CDS             4219..4908
                     /locus_tag="PK829_06110"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOU40824.1"
                     /translation="MSALRSLSGLAAVMLLLLAACGGRPLVPGSAASPQEGAVPAPTG
                     PLREEFIPGHTGNWLLEGDEVSSTAVVSEQLVITVDAPNTMQYATYTDRTFDNFVLEV
                     DAWQRSGAPESSYGILFRVTEDGQFYRFDITGNGLYMVERRAADGTWTRLVREWTPTA
                     AINQGLNVANRLKIIANGPDLSFYVNDILLTQVSDATLTGGAIALDAGSFAGKALQVA
                     FDNVNVTPGSD"
     gene            4922..5773
                     /locus_tag="PK829_06115"
     CDS             4922..5773
                     /locus_tag="PK829_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HOU40825.1"
                     /translation="MDIRRQVAAAAGSPVTDIAPLGGGCVSAVYDVRLGDGRHVVAKV
                     DESRSDTLATEGFMLRYLAAQTALPVPAVVAEGSGVLLTEYIEGDSRFDGAAEADAAE
                     RLAALHALTAPTFGFERDTLIGGLRQPNNPTAVWLDFFAEQRLIFMAGQATRAGRLPA
                     ATRARVERLAGHLDRWLVEPAAPSLIHGDVWTTNVLARGGRITAFLDPAIYFADAEIE
                     LAFVGLFGTFGRAFYQRYHALRPLAPGFFEERRDLYNLYPLLVHVRLFGGSYAGQVER
                     VLAGYGF"
     gene            5773..>6292
                     /locus_tag="PK829_06120"
     CDS             5773..>6292
                     /locus_tag="PK829_06120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU40826.1"
                     /translation="MDKATVQRLVTLNETFYSHFAAPFAASRAGPQPGYERLLAYVPV
                     GRPRVLDVGCGDGRFGRYLRAAGRDVDYAGVDFSAALLAACAVPGRFAQRDLSQPGGL
                     DGLGYFDLIVCLSTLQHIPGRHNRARVLAEMAACLAAGGHIALANWQFLSSERQRRKI
                     RPWAEAGLQASDV"
BASE COUNT          908 a         2087 c         2199 g         1098 t
ORIGIN      
        1 gacgtgaact tcagtgccac ctccgccagc atgggtgcgg ccgtcatgag aaccttccgg
       61 gcacaggcgt gggccatctg gctgttgggc ggcggcgtgc tggtgctggc cacgcgcaac
      121 ccgctctatc tggtcttgtt actggccatt tcccgcatcg tgcaggccac ctgtgccgcc
      181 gggtcaatcg ggccgcgctt gcagtatggc cggatcgccg cgttgatcct cctcttttcg
      241 acgctcttta acctgctgac cgcccacatc ggtgagacgg tgctgctgac gctgcctgct
      301 ggctggtggc tcatcggcgg gccgatcaca ttggaagcgg ccgtctatgg cttcatcacc
      361 ggcctgggcc tggtcacgct gctgtcgttc ttcgtggcct tcaacgccat tgtgccaacc
      421 ggggaactga tcgggctggc gccggcggcg ctctatgagt tggggctggt gctcttgctg
      481 gccatcacct acgtgccgga gaccggccgg caactgcaac gcattcgcga ggcgcaggcc
      541 atccgcggcc atcgcctgcg cagcctgcgc gactggcagc caattgtcat cccgctgctg
      601 gtcggcggcc tggaacgggc catgaacctg tcggagacga tggtcgcccg cggctttggc
      661 tcggtggccc acgtggccac gccctggcgc gtgcggctgg ggctgctggc cgggctggtg
      721 ctgacgctgg cgggagcact gcggttgtcc tgggcggggg cggatggctg ggcgctgatc
      781 ggcgcggggg cggtggccat tggactggcc tatcgcgccc tcagccgggg cgtcaggcgc
      841 acgcgctacc ggccgcgccc ctggacgccg gctgatacgg ccgtggtcat cggcgcgctg
      901 ctgcctctgc ttctgctgct gccacttccg gccctgggtg gggcggggcg gggttacgaa
      961 ccatacccgc ggctggcgct acccgcattt gcgccctggg cgggaacgtt gctattgggg
     1021 ttggccgcgc cggcggcatg gcaggcggtt cccgcgccgg aggacacatg accgccgatg
     1081 gcatcatcgc cattcgtcgc ctcacttatc attaccccca gcggcccacg ccggccctgc
     1141 gcgatgtcac ctgggacgtg gccgacggcg aattcgtgct cgtcgctggc gcgtccggct
     1201 cgggcaagtc gacgctgcta cgctgcctca acggccttgt gccccatttc agcggtggcg
     1261 tgctggccgg gcagatggcc gtggccggca tcgatgtcat cgccgccggg ccgcgcgtca
     1321 tgagccgcca cgttggcttc gtcgcccagg acccggaggc ccaggccgtg ctcgaccgcg
     1381 tggaggggga gatcgccttt gccctggaaa acgcggccgt gccgccggcc gagatgcacc
     1441 ggcgcgtgga gcaagccctc gacctggtgg aactgacgcc actgcgcgac cggccgctgg
     1501 atacactgtc gggcggtgag cggcagcgcc tggccatcgc cgcggcgctg gccttgcagc
     1561 cgcgcgtgct ggtgctcgac gaaccgacca gccagctcga tccgcagtcg gccgaggagg
     1621 tgctgcgggc gctggtgcgg ctgaaagcgg gcctggggct gaccattatc ctggccgagc
     1681 accggctgga gcgcatcgcc cgctatgccg accggctgac gttcctggag gacggacggg
     1741 tagtcctcga cggcccggtg cggaccacgc tgccgctcat tcccggcgcg ccgccggtcg
     1801 tggcgttggg gcgggcgctg ggctggcagc cggcgccgct gacggtcaag gaggccagac
     1861 cgctggcggc gacactgccg gtcgccgctt tccccgtgcc gcaggccacg cccgtgcccg
     1921 aaaagcccat cgcgccgcgc cttcaagtcg aaggactgcg tttttcgtat aatgggcagg
     1981 agacgctgcg tggcatcgac ctcagcgtcg ctccgggcga ggtggtggcg ctggtgggtc
     2041 gcaacggcgc gggcaagtcc acgctgctga aatgcattgt gggcctgctg cgcggccagg
     2101 cgggcgaagt gcgcgtcaat gggcgctcga cgctcggccg gcatgtagcc gacatctgcc
     2161 gcgagatcgg ctatctgccc cagaatgccg acgacctgct gttcgccgac acggtggccg
     2221 atgagatgcg cgtcacgctg cgcaaccacg gcctggcggc gacggccgtg gagcaggcgg
     2281 cgtggctggc acggctgggg ctgaccgagc tggctagggc ctacccgcgc gacctgtcgg
     2341 ccgggcagcg gcagcgggtg gctctggggg cagtgacggc gacacagccg cggctgctgc
     2401 tgctggacga gccgacgcgc ggtctggact gggcggctaa gacggccctc gtggcgctgt
     2461 ggcgtggctg gctggccgag ggcgcggcga tgctgttggt gacgcacgat gtcgagctgg
     2521 ccgctacgtt tgcccaacag acggtaattc tgagccgggg cgaggtgatc gccgccggcc
     2581 cgacgcgcga cgttctggcc ggcgcgcccc acttcgcgcc gcagatggcg cggctgtttc
     2641 ccggccgcgg ctggctgacg gtcgaagacg ctctgcgcgg gctggggcgg ccggcgagcg
     2701 gcgcaaacga gtgggaggat aaacaatgcc gcgattgacg aaaatctaca cccgaggcgg
     2761 agacgccggg cagaccagcc tctccggggg ccagcgcgtg gccaagaact cattgcgcgt
     2821 ggctgcctat ggcacggtcg atgaattgaa ttcgtatttg ggcgtggcgc tggcggcggg
     2881 gctgaccccg cggctggccg gcgtcgtggc cgagatacag aacgagctgt tccacctcgg
     2941 ctccgacctg agcttcctgg aggcggacaa ggctgaccgg ccgctgccgc agatcgagcc
     3001 gcgtcacgtc gcccggctgg aggcgctgat cgatgcgttg accgagatcg tcgggccgct
     3061 gcaaaacttc atcctgcccg gcggcgcgcc gacatctgcc cacctacatg tggcccgcgc
     3121 cgtctgtcgc cgggccgagc gggacgtgat cgccctggcc cgcgaggaag cggtgggggc
     3181 gcaggtcatg ccctacctca atcggctgtc ggacgcgctc ttcgtaatgg ctcgttacga
     3241 aaatcacgcg cgcggcatcg ccgagccgct ctgggatagc cacgcctaga tggtctgctt
     3301 ggccggaaac ggcggatcgc actacaatgc aacgtctacc ggcgtcattg ccccgcgcgt
     3361 gacggcgatt tttccagagg agaagaagtc attatgttgc ataatctggc cgtcgcggct
     3421 atcgttgtcg tcatcttgtg ggtcatcatt ctggggctgt acctcatggt cacccgccgc
     3481 cagcccgacg tcgccgcgca gatgaaggcc ttagaggagc ggctggagcg cgccgagcgc
     3541 gaggccaagc agcgctaggc agagctatgt ccacaacgct ccggcgcttc ctggtgacct
     3601 ttgtggtgct gttggccctg ctgcccctca tggcctgcct gggcgcggcc gagaccgaac
     3661 ccgacgcctg cgacgggcct gactttctgt tcgccgacaa cttcaatggc gaacttgatt
     3721 gcggctgggc tacctacgac cgcggcggcg gcacggcaac tatcgagaac gccgccatgc
     3781 aggtgactgt cggccagccg ggccaattat ggtggaccaa ccccggcaag acgtttgacg
     3841 acgttgtcat taccgccgag gcgcggcagg tggacggccc caacgacaac gcctatggtc
     3901 tcatctgccg ctatcaggac gagagcaact tctacgtctt tttggtcagc ggcgacggct
     3961 attacgccat cggcaaatac cagagcggca gcgactccgt ctcctatctg acgcccaacg
     4021 cgcagttcca gtcctccgac gccatcaacg tcggcgtggc ttccaacgag ttgcgcgtca
     4081 gttgcatcgg cagcgagctg agcctggagg tcaacggcat cccgctcatg accgtgagcg
     4141 accccacctt cgtcaacggc gacatcgggc tggcggccag cacgttgcag ccgggcacaa
     4201 ccatcgttga gtttgataat gtccgcgtta cgcagcctta gcggcctggc agccgtcatg
     4261 cttctgctgt tggccgcttg cggcgggcgg ccgctcgtgc cgggcagcgc cgcgtcccca
     4321 caagaagggg ccgtgcccgc gcccaccggg ccgttgcgcg aggaattcat ccctggccat
     4381 acgggcaact ggctgttgga aggggacgag gtcagttcga cggccgtggt cagcgagcag
     4441 ctggtcatca ccgtcgacgc gcccaatacc atgcagtacg ccacctacac ggacaggaca
     4501 tttgacaact ttgtcctgga ggttgacgcc tggcagcgtt ccggtgcgcc ggagagcagc
     4561 tacggaattc tcttccgcgt caccgaggat ggccaattct accgcttcga catcaccggc
     4621 aacgggctat acatggtcga gcggcgcgcg gccgacggga cgtggacgcg actggtgcgc
     4681 gagtggacgc cgacggcggc tattaatcag ggcctcaacg tcgccaaccg gctgaagatc
     4741 atcgccaacg ggccggactt atctttctac gtcaacgaca tcctgctgac ccaggtcagc
     4801 gacgcgactc tgaccggcgg tgcgattgcc ctcgacgccg gctcgtttgc cggcaaggcg
     4861 ctgcaagtgg ccttcgacaa cgtcaatgtg acccctggct cagactagat aggaggggag
     4921 aatggacatt cggcgtcagg tggctgccgc ggctggctcc ccggtcaccg acatcgcccc
     4981 gctgggcggc ggctgtgtca gcgccgtcta cgatgtacgc ctgggcgacg gccggcacgt
     5041 cgtggccaag gtggacgaga gccgcagcga cacgttggcc acggaagggt tcatgctgcg
     5101 ctatctggcc gcacagacgg cgctgcccgt gccggccgtg gtggccgagg gcagcggcgt
     5161 gctgctgacg gaatacatcg agggggacag ccgctttgac ggcgcggccg aggccgacgc
     5221 cgccgaacgg ctggccgcgc tccacgcgct gaccgcgccc acattcggct tcgagcggga
     5281 tacgctcatc ggcgggctgc ggcagcccaa caaccccacg gccgtatggc tggacttttt
     5341 cgccgagcag cggttgatct tcatggccgg gcaggccacc cgcgcgggac gcttgccggc
     5401 ggcgacgcgg gcgcgcgtgg agcgactggc cggtcacctc gaccgctggc tggtggagcc
     5461 agccgcgccg tcactcatcc acggcgacgt ctggacgacg aacgttttgg cccgcggcgg
     5521 gcgcattacc gcctttctcg acccggctat ctatttcgcc gatgcggaga ttgaactggc
     5581 tttcgtcggc ctgtttggca cgttcggtcg cgccttctac cagcgctacc acgctctgcg
     5641 cccccttgct cccggcttct tcgaggagcg gcgcgatctt tacaatcttt atccgttgct
     5701 cgttcacgtc cgcctctttg gcggcagcta tgccggccag gtggagcgcg tgctggccgg
     5761 ctacggcttc tgatggataa ggcgactgtc cagcgactgg tgactctgaa cgagacgttc
     5821 tacagccact tcgccgcgcc gtttgccgcc agccgggccg gaccgcagcc gggctatgag
     5881 cgcttgctgg cctacgtgcc cgtggggcgg ccgcgcgtcc tggatgttgg ctgtggtgac
     5941 ggccgcttcg ggcgctactt gcgcgccgca gggcgcgatg tggattacgc cggcgtggac
     6001 ttctccgccg ctctgctggc cgcgtgcgcc gtgcccggcc ggttcgcgca gcgcgacctg
     6061 agccagcccg gcggcctcga tggtctgggt tacttcgacc tcatcgtttg cctatccacg
     6121 ctgcaacaca tccctggccg gcacaaccgc gcccgcgtgc tggcggagat ggccgcgtgc
     6181 ctcgctgccg gtggccacat tgccctggcg aactggcagt ttctgagcag tgaacgtcag
     6241 cggcgcaaga ttcggccgtg ggcggaggcg gggctgcaag cgagcgacgt gg
//
LOCUS       DAOSMQ010000140         6257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12599_length_6257_cov_4.537246, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000140 DAOSMQ010000000
VERSION     DAOSMQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12599_length_6257_cov_4.537246"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PK829_06125"
     CDS             <1..937
                     /locus_tag="PK829_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU40827.1"
                     /translation="LGHYALNCQAPDAGNMEILIEYGTPAQQERFLRPLLDGDIRSCF
                     SMTEPENAGSNPTWLSTTATTDGDDYIIDGHKWFTTGGDGAAFAIVMAVTDSAAPKHQ
                     RASMIIVPTDTPGFQIVQNTSVMGERGQGWASHAEIRYDACRVPQSNRLGAEGMGFII
                     AQQRLGPGRIHHCMRWLGICQRAFDLMCRQAALREIAPGQPLAEMGQVQTWVAESRAE
                     IDAARLLVLQTAQQIDRDGAHAARDSISLIKFHVAKVLQDVLDRAIQVHGAMGMTDET
                     PLAFWYRHERAARIYDGPDEVHKRAVARRILGRYG"
     gene            958..1506
                     /locus_tag="PK829_06130"
     CDS             958..1506
                     /locus_tag="PK829_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU40828.1"
                     /translation="MNSVQYSVSSIQWTVVVELLEQTVFGVGDAMTERLPYAKNFREL
                     VVYQKSRRLAREVFHLSKRFPKEETYSLTDQLRRASRAIGANIAEAWGKRSYERHFLS
                     KLTDADAEQYETQHWLEIAIDCGYIAATDGRPLIAACEEIGRLLGGVMARSAAFCNPT
                     ALREPLVEYFILEAANDDQVEH"
     gene            1551..2597
                     /locus_tag="PK829_06135"
     CDS             1551..2597
                     /locus_tag="PK829_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOU40829.1"
                     /translation="MDDVIDVRPDERFDEARLAGYLAGRLPGSDHVPAVSQFGGGAAN
                     LTYLLDYGTHEYVLRRPPLGPVAASAHDMSREYRVLSVLWQALPYAPRAFLFCEDDAV
                     IGAPFFIMERRHGVMVRRAMPDIFADQPDAAGRMSAALVDALAEFHAVDYDDLGLGDL
                     GKPQGFLERQIEGWYRRWQGARTADLTNMDRVYRWLRDSCPTGGAVSLVHNDYKLDNV
                     MLAADDPGRVVAIFDWDMCTLGDPLSDLGALLTYWTEPADPAYLQATAMMPLGDLGFL
                     TRDELVARYEARSGRPVDNIRFYQALGLFRLTVIIAQIYIRYRRGQTQDARFAAMEQM
                     IPLLARRAAEVAWE"
     gene            2735..3574
                     /locus_tag="PK829_06140"
     CDS             2735..3574
                     /locus_tag="PK829_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU40830.1"
                     /translation="MPYHSIFRPDLFAGQTYVVTGGGTGIGRAVAHELASLGAHVVLA
                     ARTEENLSRVAGEIRAAGGSAGYVVCNIREEEQVRALFEHVLAERGGLDGLVNNAGGQ
                     FISPAEMISLKGWRAVVETNLTGTFLMCREAFNRHMGAHGGAIVNMLIEMWRGFPGMA
                     HSAAARAGIENLTKTLAVEWARAGVRVNAVAPGLISSSGLEHYPEAVRPMIAQNIRDI
                     PLKRMGTEAEVSAAIVFLLSPAAAFITGESIRIDGGGSLWRKTWEVPEHENAPAGYEG
                     VEQ"
     gene            complement(3604..3945)
                     /locus_tag="PK829_06145"
     CDS             complement(3604..3945)
                     /locus_tag="PK829_06145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOU40831.1"
                     /translation="MVDQGDVYWIDIGEPRGSEPGYRRPYVVIQNNTYNHAAINTVIV
                     CALTSNVRRSRIPGNVLLNAGEAGLPKASVVVVTQLYTVDKRDLIEKIGVVSASQVRQ
                     ILQGLGEVLTP"
     gene            complement(3939..4196)
                     /locus_tag="PK829_06150"
     CDS             complement(3939..4196)
                     /locus_tag="PK829_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40832.1"
                     /translation="MTTIKTAVSIEEGLFNQADELAKEMQVSRSRLFALALENYVSQH
                     RSRKITEILNEEYADGPTAEELETLEAMRRYQAILWKDEPW"
     gene            complement(4241..5044)
                     /locus_tag="PK829_06155"
     CDS             complement(4241..5044)
                     /locus_tag="PK829_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOU40833.1"
                     /translation="MTNPPEPGFTRIPYPRSRAFVIDSAHVASNRHMIHGLVEFDVTK
                     ARAMLREHKGRTGESLSFTAFVLHCVGAAVAEDRMVHAYRDWRGRLVLFDDVDINTIV
                     EIELEGRRFPLAHVVRATNRRSVRELHNEIRSVQAKGNRNYNDRAVRLLRWYARLPGP
                     LRRVAYRIGMASPYRMKAIGGTASVTAVGMFGEGSGWGIPIPLYTLNLTLGGTATKPA
                     VIAGQIVPREMLSVTLSFDHVVVDGAPAARFAERLKKLVEGAAGLDEIV"
     gene            5315..>6257
                     /locus_tag="PK829_06160"
     CDS             5315..>6257
                     /locus_tag="PK829_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOU40834.1"
                     /translation="MSQFAHGGELVARSLAAQGVSTIYTLCGGHVAPIYDGCLNHNIA
                     VIDFRHEQAAAHAADAHARLTRSVGAPNVGVALVTAGPGVTDAVTGVANAYQARSPLL
                     LLGGAAPLKTKGMGALQEMPQVDMFRTFTKASFTIERTEDIPARLAEAYTAALSGRPG
                     PVFVELPFDVLFNAIDAPAQMPDRVAAAPAPPPVSAVQAMFDLLRAAERPLIIAGTQV
                     YWDDAGDALRDLTDAAAIPVFTNGAGRGTLSMAHPHCFKAARGKALRAADVVLLIGTP
                     LDFRLKYGREDWNPASKLIQIENDAAELNHNRQADVAV"
BASE COUNT         1144 a         2063 c         1956 g         1094 t
ORIGIN      
        1 cctgggccac tacgcgctga actgccaggc ccccgacgcc ggcaacatgg agattctcat
       61 tgagtatggc acgccggcgc agcaagagcg cttcctgcgt ccgctgctcg acggcgatat
      121 ccgcagttgc ttctccatga ctgagccgga gaacgccggc tctaacccca cctggctgag
      181 caccacggcg acaacggacg gcgacgacta catcattgac ggccacaagt ggttcaccac
      241 cggcggcgat ggcgccgcct ttgccatcgt catggccgta accgactccg ccgcgccgaa
      301 gcaccagcga gccagcatga tcatcgtgcc cacggacacg cccggcttcc agatcgtaca
      361 gaacacgtcg gtgatgggcg aacgcggcca gggctgggcc agccacgccg agattcgcta
      421 tgacgcctgc cgcgtgcccc agagcaaccg gctgggcgcg gagggcatgg gcttcatcat
      481 tgctcagcag cgcctggggc caggccgcat ccaccattgc atgcgctggc tcggcatctg
      541 ccagcgggcc ttcgacctga tgtgccggca ggcggcgctg cgcgagatcg cgccgggcca
      601 gccgctggcc gagatgggcc aggtgcagac gtgggtggcc gagagccgcg cggagatcga
      661 cgcggcgcgg ctgctggtct tgcagacggc gcagcagatc gaccgcgacg gcgcgcacgc
      721 cgcccgcgac agcatctcgc tgatcaagtt ccacgtggcc aaagtgctcc aggacgtgct
      781 cgaccgggct attcaggtgc atggcgcgat gggcatgacc gacgagacgc cgctggcctt
      841 ctggtatcgc cacgagcgcg ccgcgcgcat ctacgacggc cccgacgaag tccacaagcg
      901 cgccgtggcg cggaggattt tggggaggta tgggtagttt tcagtgacca gtgtccagtg
      961 aacagtgtcc agtattcagt gtccagtatt cagtggacag ttgttgtaga acttttggag
     1021 caaactgttt ttggtgtggg ggacgccatg acggagaggt tgccgtatgc aaaaaacttc
     1081 cgcgagttgg tcgtgtatca gaagtcaagg cgacttgcgc gtgaggtgtt tcacttgtcg
     1141 aagcgatttc caaaagagga aacctattca ctgaccgatc agttgcgtcg tgcctccaga
     1201 gcgattgggg cgaacattgc tgaggcctgg ggcaaaagga gctatgaaag gcactttctc
     1261 agcaaactga cagatgccga tgcggaacag tacgagacac aacactggct ggaaattgcc
     1321 attgattgtg gttatattgc agccactgat ggtcgcccgc taatcgcagc ctgcgaggag
     1381 atcgggcgct tactcggtgg ggtaatggcg cggtcagccg ccttctgtaa tcccaccgcc
     1441 cttcgagaac ccttggtcga atacttcatc ttagaagcag ccaacgacga ccaagttgaa
     1501 cactgaacac tggatactga acactggata ctggatactg gatactcctt atggacgacg
     1561 taatagacgt tcgccccgac gagcgcttcg acgaagcgcg attggccggc tatctggccg
     1621 gccgcctgcc cggcagcgac cacgtgccgg ccgtcagcca gttcggcggc ggcgcggcca
     1681 acctgaccta cctactcgac tacggcacgc acgagtacgt cctgcgccgg ccgccgcttg
     1741 ggccggtggc cgcctcggcc cacgacatga gccgcgagta ccgcgtcctg tcggtgctgt
     1801 ggcaggcgct gccctacgcg ccgcgggcct tcctcttttg cgaggacgac gcggtcatcg
     1861 gcgcgccgtt cttcatcatg gagcggcggc acggcgtcat ggtccgccgc gccatgcccg
     1921 acatcttcgc cgaccagccc gacgccgccg gccgcatgag cgcggcgctg gtagacgccc
     1981 tggctgagtt ccatgccgtc gattacgacg acctgggcct gggcgacctg ggcaagcccc
     2041 agggcttcct ggagcggcag atcgagggct ggtatcgccg ctggcagggc gcgcggacgg
     2101 ccgacctgac caacatggac cgcgtctatc gctggctgcg cgacagctgc cccactggcg
     2161 gcgcagtttc tctcgtccac aatgactaca agctcgataa cgtcatgctg gccgccgacg
     2221 atcctggccg cgtcgtggct atcttcgact gggatatgtg cactctgggc gacccactgt
     2281 ccgacctcgg cgcgctattg acctactgga cggagccggc cgaccccgcc tacctgcaag
     2341 ccacagcgat gatgcccctg ggcgacctgg gcttcctgac ccgcgacgaa ttagtagccc
     2401 gctacgaggc caggagcggc cggcccgtgg acaacatccg cttctaccag gccctcggcc
     2461 tcttccgcct gacagtcatc atcgcccaga tctacatccg ctatcgccgc ggccagacgc
     2521 aagacgcgcg tttcgcggcc atggagcaga tgatcccgct gctggcccgg cgcgcggccg
     2581 aagtggcgtg ggaatgaccg ccgactgtcg accgctgacc gctgacgaca gacgacagac
     2641 cacggaccac agccgctcca caaccgaaca ctggacactg gatactggat actgaatact
     2701 gacccactgc cctactgaca actgacaact acccatgccc taccactcca tcttccgccc
     2761 cgacctcttc gccgggcaga cctacgtcgt cactggcggg ggcacgggca tcggccgggc
     2821 cgtcgcccac gagttagcct cgctgggcgc acacgtcgtt ctggcggcgc gaacggagga
     2881 gaacctgagt cgcgtcgctg gcgaaatccg cgccgcgggc ggcagcgccg gctacgtcgt
     2941 ctgtaacatt cgcgaggagg agcaggtgcg ggcgctcttc gagcatgtgc tggccgagcg
     3001 cggcggtctg gatggcctag tcaacaacgc cggcggccag ttcatcagcc cggcggagat
     3061 gatctcgctg aagggctggc gcgccgtggt ggagacgaac ctgaccggca ctttcctgat
     3121 gtgccgcgag gccttcaacc gccacatggg cgcgcacggc ggggccatcg tcaacatgct
     3181 catcgagatg tggcgcggct tccccggtat ggcccattcg gccgcggccc gcgccggcat
     3241 cgagaacctg accaagacgc ttgccgtcga gtgggcgcgg gcgggcgtgc gcgtcaacgc
     3301 cgtggccccc ggactcatct cctccagcgg gctggagcac tacccggagg cggtgcggcc
     3361 gatgatcgcg cagaacatcc gcgacatccc gctcaagcgc atgggcacgg aggccgaggt
     3421 cagcgcggcc atcgtcttcc tgctgtcgcc ggcggcggcg ttcatcactg gcgagtcgat
     3481 tcgcatcgat ggcggcgggt cactgtggcg caagacgtgg gaagtgccgg agcatgagaa
     3541 tgcaccggcg gggtatgagg gggtcgaaca gtagccgtat gaattgtaac ggcctgaaac
     3601 atactaaggg gttaacacct cgccaagccc ctgcaaaatc tgtcgaacct gactcgcaga
     3661 caccacgccg attttttcga tgaggtcccg tttatctact gtgtacaact gagtgacgac
     3721 aacgacactg gctttcggca atcccgcttc gcctgcgttt aacaatacat taccgggtat
     3781 gcgtgagcgg cgaacgttgg aagttaatgc acacacaatc acggtgttga ttgcagcgtg
     3841 gttataggtg ttgttctgga tgacaacgta gggccgacga tagcccggct cagaccctcg
     3901 cggttcaccg atgtcgatcc agtatacatc accctgatct accatggctc atccttccaa
     3961 agtattgcct ggtagcggcg catagcctca agggtctcca attcttctgc cgtcggccca
     4021 tcggcatact cttcattgag aatttcagtg atcttgcgac tgcgatgttg tgagacgtaa
     4081 ttctccagtg cgagtgcgaa aaggcgactc cgagaaactt gcatttcctt ggccaattca
     4141 tctgcctggt tgaacagacc ctcctcaatt gaaaccgcgg ttttgatagt ggtcatacca
     4201 ttagtataac acataatcat accaccatca atactacatt tcatactatc tcgtcgagtc
     4261 ctgctgcccc ctcgactagc ttcttcaacc gctccgcaaa ccgcgccgcc ggcgcgccat
     4321 cgaccacgac gtggtcgaat gacagcgtca cactcaacat ctcgcgcggg acaatctgac
     4381 cagctatgac cgctggtttc gtggcggtgc cgcccagggt gagattcagg gtgtaaaggg
     4441 gaatagggat gccccagccg ctgccctcgc cgaacatgcc gacggccgtc acgctggccg
     4501 tcccgccgat ggctttcatc cggtagggac tggccatccc gatccggtag gctacgcggc
     4561 gcagtggccc cggcagtcgg gcgtaccaac gcagcaggcg cacggcccgg tcgttgtagt
     4621 tgcggttgcc tttcgcctga accgaccgta tctcgttgtg cagctcgcgc acggagcggc
     4681 gattggtggc gcggacaacg tgggccaacg ggaaacggcg gccctccagc tcgatctcga
     4741 caatggtgtt gatgtccaca tcgtcgaaca agacgagccg gccgcgccag tcgcggtagg
     4801 cgtgaaccat acggtcctcg gccacggccg cgccgacgca gtgcagcacg aacgccgtga
     4861 atgagaggga ttcgccggtg cggcccttgt gttcgcgcag catcgctcgc gccttcgtca
     4921 cgtcgaactc aaccaggccg tggatcatgt gcctgttgct ggcgacgtgg gccgagtcga
     4981 tgacgaacgc gcgcgaacgc ggatagggta tccgtgtgaa gcccggctca ggtggattgg
     5041 tcatctcagc ctccgccgca agggtaatga aagcggagtg ctggtgcaac tcttgactgg
     5101 aagcgatgcg cgtgacagtc gaacttggcg tggggcgcgc agaaataggc ccgcaaccgc
     5161 gcccctgagc gtggcaacgg ggcgacgcac tggctgccag tgcgtcgccc tttttatctg
     5221 gcgcggcgtt gcgtcccgcc caccgttgcg ctacgctacc ggcaaccgat ttcaggtgtt
     5281 caggccttca gcccacactg gcaaggagcg ctccatgtcc caattcgccc acggcggaga
     5341 actcgtcgcc cgctcgctgg ccgcgcaggg cgtcagcacg atctacaccc tttgcggcgg
     5401 ccacgtcgcg cccatctacg acggctgcct gaaccacaac atcgccgtca tcgacttccg
     5461 ccatgagcag gccgctgccc acgccgccga cgcccatgcc cggctgacga gaagcgtcgg
     5521 cgctcccaac gtgggcgtgg ctctggtgac agccgggccg ggcgtcaccg atgccgtcac
     5581 cggcgtggcc aacgcctatc aggcccgcag cccgctgctg ctgctgggcg gagccgcgcc
     5641 gctgaagacc aagggcatgg gcgcgttgca ggagatgccc caggtggaca tgttccgcac
     5701 cttcaccaag gccagcttca ccatcgagcg caccgaggac atccccgcgc ggctggccga
     5761 ggcctacacc gccgctctca gcggacgccc cgggccggtc ttcgtcgaac tgccgttcga
     5821 cgtgctcttc aacgccattg acgcgccggc gcagatgccc gaccgcgtgg ccgccgcgcc
     5881 cgcgccgccg ccggtcagcg ccgtgcaggc catgttcgac ctattgcgcg ccgccgagcg
     5941 gccgctgatc atcgccggga cgcaggtcta ctgggacgac gccggcgacg ccctgcgcga
     6001 cctgaccgac gcggcggcca tccccgtttt caccaacggc gccggccgcg gcacgctgtc
     6061 gatggcccat ccccactgct ttaaggcggc gcggggcaag gcgttgcggg cggccgatgt
     6121 tgtcctgctc atcggcacgc cgctggactt ccgcctgaag tatggccgcg aggactggaa
     6181 cccggccagc aagctcatcc agatcgagaa cgacgccgcc gagctaaacc acaaccgcca
     6241 ggccgacgtg gccgtgg
//
LOCUS       DAOSMQ010000141         6255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12609_length_6255_cov_3.696290, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000141 DAOSMQ010000000
VERSION     DAOSMQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12609_length_6255_cov_3.696290"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1163
                     /locus_tag="PK829_06165"
     CDS             147..1163
                     /locus_tag="PK829_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011840452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOU40835.1"
                     /translation="MYEVIDDLTRWLAAGQADIALATVLTTWGSAPRRAGAKMAFTAG
                     GAALSGSVSGGCVEGAVIAAGEETLADGRPRRLRFGVADETAWGVGLACGGTIEVFVE
                     RLDRATYAAARRWLDAGASGALLTVVAGPEVLLGRKAIVEDGQVIAGSLGAALGEMLP
                     VAVPPEPQLLAAAGGVELFVDPIHPAPALFLIGGAHIAVALARLAAVVGYRVVVIDPR
                     RAFGSAERFPDVDHIDHRWPDKALADLRPGASAAVVTLSHDPKIDDPALRAALAGDAF
                     YIGALGSRKTHAARRDRLVAAGLSEAQLARIHAPVGLDLGAQTPEEIALAIMAEVVKA
                     WHNR"
     gene            complement(1280..2293)
                     /gene="galE"
                     /locus_tag="PK829_06170"
     CDS             complement(1280..2293)
                     /gene="galE"
                     /locus_tag="PK829_06170"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOU40836.1"
                     /translation="MRILVTGGAGYIGSHTVLELLQAGHDVVVLDNLINSQEESLRRV
                     ARLAGRAPTFHCVDLLDRTAVDAVFAAAPVDAVIHFAALKAVGESVAQPLRYFHNNLT
                     GMLHLLQAMEAHDVRCLVFSSSATVYGDPQQMPITEDAPLSAVNPYGRTKLVAEDMLR
                     DLYRADPRWDIALLRYFNPVGAHRSGQIGEDPNGIPNNLVPYIAQVAVGRLPYLRVFG
                     NSYPTPDGTGVRDYIHVVDLAQGHLRALTKLGEHPGLVTYNLGTGRGYSVLEVVAAFE
                     RASGRPIPVQIHPPRPGDAAICYADPSRAARELAWVAENDLDDMMADTWRWQAGNPNG
                     YGE"
     gene            complement(2453..3151)
                     /locus_tag="PK829_06175"
     CDS             complement(2453..3151)
                     /locus_tag="PK829_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOU40837.1"
                     /translation="MSQARVLYDLQQIDTEIRTKKQRLGEVLRLQKEPPALLAAREKA
                     ATADADLSRWRARHKDLTLEIAGVSDKAKRSEERLYSGVVKSPKELNDLQHEVEALGR
                     RRATLEDEALQVMMTVDERQASSSAAATERDHLADEFAASVVHLQAEQQTLALQLNRL
                     LDKRKKQVMLAQPALLKVYDQLIQEKRGLAVAGLRVNKCQGCLVTLSASTIRAADEGK
                     LVRCDSCDRILCPL"
     gene            3226..4290
                     /locus_tag="PK829_06180"
     CDS             3226..4290
                     /locus_tag="PK829_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOU40838.1"
                     /translation="MVARMMELGEQAYTEADAEWDAFVAAHPHGSLLQTTNWARLKNR
                     FGWTSQRVWLRRDGRLVAGAQVLFRSLAFGVVKIAYIPHGPLADWDNEEQLAVLFNQI
                     DQAAYRRGAGLLKFEPRLWQDECPPDQWAALYRRHGGVPSADTIQPPRTVVVDLRPSE
                     DDILAAMKQKTRYNIRLAEKKGVTTRLATAADLPAFNRLMTATGARDRFAVHQPQYYA
                     AALELFAPQNAALWLAEYEGRPLAGVMVFTWGSSAAYLYGASSEQERARMPAYAAQWA
                     AMRWARARGCQSYDLWGVPDADETTLEAGFTERQDGLWPVYRFKRGFGGEVRRTVGAA
                     DRVYNGLLARLYAWRRGRQA"
     gene            complement(4310..5395)
                     /gene="cax"
                     /locus_tag="PK829_06185"
     CDS             complement(4310..5395)
                     /gene="cax"
                     /locus_tag="PK829_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HOU40839.1"
                     /translation="MDSVLKRIEHYTGLNLVLLIFALFIPLAFILEFSHASPTLILLA
                     AALGIVPLAGMIGEGTEALAERVGQRAGGLLNATLGNAAELIIAVMALRRGLIQLVLA
                     SITGSILGNLLLVLGLSILVGGLKHGTQRFNRANAGIDATLLILSVFALGIPSFFNLA
                     IEPDFQAVEFLSIGAAVAILIMYVLTIIYSFTSHTTEADPQAREAHAPTGWGPRHAVI
                     VLVAAVALIAVLSEFLVGAVEPVTEALGLSEFFLGIILIPLVGNAAEHFVAVQVALKN
                     KMDLSLSIAIGSSLQIALFVAPLLVFISLALGNPMPLEFTLYEVLAVAAAALIAAFVS
                     LDGESIWLEGSMLLVLYAILAVAFFFL"
     gene            5694..>6255
                     /locus_tag="PK829_06190"
     CDS             5694..>6255
                     /locus_tag="PK829_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOU40840.1"
                     /translation="MSTDTARRAATFKREESPATWDQALARLPYAHALQSWAWGQFKA
                     RWGWQALPLSLSVGGNGWEAIAAAHVLRRNLPRTPWCILYVPKGPALDYADVTLRRHV
                     LAQLEALARRERAIFIKIDPEVVRNWGLEHERSSPLGAAVMRDLSARGWRPSAEQIQF
                     RNTVELPLESSDEQLLAAMKPKTRYNI"
BASE COUNT         1050 a         2023 c         2169 g         1013 t
ORIGIN      
        1 tcaggctgtc gtcgccggcc acagccacag cctaacaggc tgtgctacag gctgtgctac
       61 gggctgtggg ccgaccacta aaaaccctgg gccatccgcg gtcgctattg aagttacgct
      121 cacagtccac tagaatcaac ggcacaatgt acgaagtcat cgacgaccta acgcgctggc
      181 tggcggccgg ccaagccgac atcgctctgg ctacggtgct gacgacgtgg ggttctgcgc
      241 cgcgccgggc gggggccaag atggccttca ccgccggcgg cgcggccctc agcggctcgg
      301 tcagcggcgg ctgtgtcgag ggcgcggtca ttgccgccgg cgaggagacg ctggccgacg
      361 gccggccgcg gcggctgcgc ttcggcgtgg ccgatgagac ggcctggggc gtgggcctgg
      421 cctgcggcgg caccattgag gtctttgtcg agcggctcga ccgtgccacc tacgccgccg
      481 cccgccgctg gctcgacgcc ggcgcaagcg gcgcgctgct gacggtcgtg gccgggccgg
      541 aagtgctgtt ggggcgcaag gcgattgtcg aggacggcca ggtgatcgcc gggtcgctcg
      601 gcgcggcgct gggcgagatg ttgccagtgg ccgtgccgcc ggagccgcaa ctcctggccg
      661 cggccggggg cgtcgagcta ttcgtcgatc ccatccaccc cgcgccggcg ctgttcctca
      721 tcggcggggc gcatattgcc gtggccctgg cgcggctggc ggcggtggtg ggctatcgtg
      781 tcgtggtcat cgatccgcgc cgcgcctttg gcagcgccga gcgcttcccc gacgtcgacc
      841 acatcgacca ccgctggccc gacaaggccc tggccgacct gcgaccgggg gcgagcgcgg
      901 ccgtcgtgac cctcagccac gaccccaaga tcgacgaccc ggccctgcgg gcggcgctgg
      961 ccggcgacgc cttctacatc ggcgcgctgg gcagccgcaa gacccacgcc gcccggcgcg
     1021 accggctggt cgcggccggc ttgagcgagg cccaactggc ccgcatccac gcccccgtcg
     1081 gcctcgacct cggcgcgcag acgcccgaag agatcgcgct ggccatcatg gcggaagtgg
     1141 tgaaggcatg gcacaaccga tagacagccc gtttctcgcc tctattcgac atcaagttgg
     1201 cgatgggcta gctggccaac gaagctaacc ccgctttgcg cctcacagac ctactgacct
     1261 actgcttact gcctactgct tactctccat acccattcgg gttccccgcc tgccagcgcc
     1321 acgtgtcggc catcatgtca tccaggtcgt tctcggccac ccaggccagt tcgcgcgccg
     1381 ctcgcgaggg gtcggcgtag cagatagccg cgtcgccggg gcgcggcggg tgaatttgca
     1441 ccggaatggg ccggccgctg gcgcgttcga aggcggccac cacctccagc acgctgtagc
     1501 cgcggccggt gcccaggttg taggtgacca ggccgggatg ctcgcccagc ttggtcaggg
     1561 cgcggagatg cccctgggcc agatcgacga cgtggatgta gtcacgcacg ccggtgccat
     1621 cgggggtggg atagctgttg ccgaaaacgc gcagataggg caagcggccg acggccacct
     1681 gggcgatgta gggcacgagg ttgttgggga tgccgttggg gtcttcgccg atctgcccgc
     1741 tgcggtgcgc cccgacgggg ttgaagtagc gcaacagggc gatgtcccag cgcgggtcgg
     1801 cgcggtagag atcgcgcagc atgtcctcgg cgacgagctt ggtgcggccg taggggttaa
     1861 ccgccgacag cggcgcgtcc tcggtgatgg gcatttgctg cgggtcgccg tagactgtcg
     1921 ccgaggagct gaagacgagg caacgcacgt cgtgggcctc catcgcctgc aacaggtgca
     1981 gcataccggt gagattgttg tggaaatagc gcaatggctg ggcgaccgac tcgccgacgg
     2041 cctttagcgc ggcgaagtgg atgacggcgt ccaccggcgc ggcagcgaag acggcgtcca
     2101 ccgcggtccg gtcaagcaga tcaacacagt ggaaggtggg cgcgcggccg gccaaccggg
     2161 ctacgcgccg cagtgactcc tcctggctgt tgatcagatt gtcgagaacg acgacgtcgt
     2221 gcccggcctg tagtagctcc agcacggtgt ggctgccaat gtaaccggcg ccgccggtga
     2281 ccaagattct catcgtctat gctcctgagt gtagagaggt tcagcgttag cgtacgctac
     2341 tactcttaca gcctgtctgg cgaaagggcg cagtgggcca gagggcggcc acaactctgg
     2401 tcagccatgc ggcacgacgg tggggatagg gcgcggaggt gcctggccgc ggctagagcg
     2461 ggcagaggat gcggtcgcag ctatcgcagc gcaccagctt gccctcatcg gcggcgcgga
     2521 tggtgctggc cgacagggtg accaggcagc cctggcactt gttgacccgc agcccggcca
     2581 cggccagccc acgcttctcc tggatgagtt ggtcgtagac cttcagcagc gccggctggg
     2641 ccagcatgac ctgcttcttg cgcttgtcca gcaggcgatt gagctgtagg gccagcgtct
     2701 gttgttcggc ctgcaagtgg acaaccgagg ccgcgaattc gtcggccagg tggtcgcgtt
     2761 ccgtcgccgc cgcgctgctg ctggcctggc gctcatcgac ggtcatcatc acttgtagcg
     2821 cttcgtcctc taacgtggcc cggcggcggc ccagcgcttc gacctcgtgt tgcaagtcgt
     2881 tgagttcttt gggactttta acgacgccgg agtagagccg ttcttccgag cgtttggcct
     2941 tgtcgctgac gccggcaatt tcgagggtca ggtccttgtg gcgagcacgc cagcggctca
     3001 gatcagcgtc ggccgtggcg gccttctcgc gggcggccag cagcgccggc ggctcctttt
     3061 gcaggcgcaa cacttcgccc agccgctgct tcttcgtccg aatctcggta tcgatctgct
     3121 gtaggtcgta aagaactcgt gcctggctca tcgcgcctcc cgctgactgc cggtcattgt
     3181 aacacatcga ctccggatga cgaacgcgga tataatcccg gcacgatggt ggctagaatg
     3241 atggaactgg gcgagcaggc ctataccgag gccgacgcgg agtgggacgc ttttgtggct
     3301 gcccaccccc acggcagcct gctacagacg acgaactggg cgcggctgaa gaatcgcttc
     3361 ggctggacat cgcagcgcgt ctggctgcgg cgcgacggcc gcctcgtggc cggggcgcag
     3421 gtgctctttc gctcgctggc ctttggcgtc gtcaagatag cctacattcc ccacggcccg
     3481 ctggctgatt gggacaacga agagcaattg gcggtgttgt tcaaccagat cgaccaggct
     3541 gcctaccgcc gtggcgcggg cctgctgaag ttcgagccgc gcctgtggca agacgagtgc
     3601 ccacccgacc agtgggcggc gctctaccgg cggcacggtg gcgtcccttc ggccgacacc
     3661 atccagccgc cgcgcaccgt ggttgtggac cttcggccgt cggaggacga tattctggcg
     3721 gccatgaagc agaaaacgcg ctacaacatc cgcctggccg agaagaaggg cgtgacgacg
     3781 cgcctggcca cggccgccga cctgccggct ttcaatcgtc tgatgacggc cacgggcgcg
     3841 cgcgaccgct tcgccgtcca ccagccgcag tactacgccg cggcgctgga gctattcgcc
     3901 ccgcagaacg ccgccctgtg gttggccgag tacgagggcc ggccgctggc cggggtgatg
     3961 gttttcacgt gggggtcgtc ggccgcctac ctctatggcg cgtcgagcga gcaggagcgg
     4021 gcgcggatgc ccgcctacgc cgcccagtgg gcggccatgc gttgggccag ggcgcgcggc
     4081 tgccagagct atgacctgtg gggcgtgccc gatgccgacg agacgacgct ggaagccggc
     4141 ttcactgagc ggcaagacgg gctgtggccg gtctaccgtt tcaagcgtgg cttcggcggc
     4201 gaggtgcggc ggacggtggg cgcggccgat cgcgtctaca acggcctgct ggctcgcctc
     4261 tacgcctggc ggcgcgggcg ccaggcgtag ggcgggttgc caacccgccc tacaggaaga
     4321 agaacgccac cgccaggatg gcgtagagga cgagcagcat tgacccctcc agccagatgc
     4381 tctcgccgtc cagcgaaacg aacgcggcga tgagcgccgc cgcggccacg gccagcacct
     4441 cgtagagcgt gaactccagc ggcatcgggt tgcccagcgc caggctgatg aacaccagca
     4501 gcggggcgac gaagagggcg atctgcaggc tggagccgat ggcaatgctc aggctcaggt
     4561 ccatcttgtt cttcagggcc acctgcacgg cgacaaagtg ttcggcggcg ttgcccacca
     4621 gcgggatgag aatgatgccc aggaagaact cgctcagccc cagcgcctcg gtcaccggct
     4681 caacggcgcc aacgaggaac tccgacagca cagcgatcag agctacggcc gcgaccagga
     4741 cgatgaccgc gtgccgcggc ccccagccgg tgggcgcgtg ggcttcgcgg gcctggggat
     4801 cggcctcggt cgtgtgggag gtgaacgagt agatgatcgt cagcacgtac atgatcagga
     4861 tggccacggc cgcgccgatg ctcaggaact ccaccgcctg aaagtccggc tcgatggcca
     4921 ggttgaagaa cgaagggatg cccagggcaa agacggacag aatcagcaac gtcgcgtcaa
     4981 tgccggcgtt agcccgattg aaacgctgcg tgccgtgctt gaggccgccc acgagaatcg
     5041 ataggcccag taccagcagc aggttgccga ggatagagcc ggtgatggag gccagcacca
     5101 actggatcag gccgcggcgc aaggccatga cggcgatgat cagctcggcg gcgttgccca
     5161 gcgtggcgtt gagcagacca ccggcgcgct ggccaacgcg ctctgccagc gcttccgtac
     5221 cctcgccgat catgcccgcc agagggacaa tgcccagagc cgcggccagc aggatgagcg
     5281 tcgggctggc gtggctgaat tcgagaatga aggccagggg aatgaacagg gcaaagatga
     5341 gtagcaccag gttaagaccg gtatagtgct caatgcgttt caagacggaa tccatgtatc
     5401 cctccacagt gagataagct caggcgggat tgtaacagaa gcacaaccag gctcaaccga
     5461 aacgggatgg caatggcacg cagatgacat gcagggcttt tccaaagtca ccccaggcgc
     5521 atcgacctga caggcgggcg accgcgattg gccgcctacc tgtcaggtca atagtcgcta
     5581 gacgactttg gcaaagccct gcattcttca tcttgcccta tgcgcggccg gggcaaacgg
     5641 ggtaagatag gcgcgctgcg gcgagaggcc gccgcttttt gtctatggcc ctcatgagca
     5701 ccgacaccgc tcgccgcgcg gcgacgttca agcgcgagga atcgccggcc acctgggatc
     5761 aggccctggc ccgcttgccc tatgcccacg ctttgcagag ctgggcatgg gggcagttca
     5821 aggcgcgctg gggctggcag gcattgccgc tgagcctgtc tgtgggcggc aatggctggg
     5881 aggccatcgc cgcggcccac gtcctgcggc gcaacctgcc gcggacgccg tggtgcatcc
     5941 tctacgtgcc caaagggccg gcgctcgact acgccgacgt gactctgcgc cgccacgtcc
     6001 tggcgcaatt ggaggccctg gcccggcgcg agcgggccat tttcatcaag atcgacccgg
     6061 aagtggtgcg caattggggg ctggaacatg agcggtcgtc gccgttgggc gcggcggtga
     6121 tgcgtgacct gagcgcgcgt ggctggcgac cctcagccga gcaaatccag ttccgcaaca
     6181 ctgttgagtt gccgctggaa agcagcgacg agcaactgct ggcggccatg aagcccaaga
     6241 cgcgctacaa catcc
//
LOCUS       DAOSMQ010000142         6233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12659_length_6233_cov_4.779378, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000142 DAOSMQ010000000
VERSION     DAOSMQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12659_length_6233_cov_4.779378"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PK829_06195"
     CDS             complement(<1..831)
                     /locus_tag="PK829_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOU40841.1"
                     /translation="MSVLPLTSMNRPESLRAYRWRVAPEMPADSPLQSVGDAVHPVLA
                     RILHNRGLAQPAQVQAFLSHHYLLSRDPYQLADMDRAVDRLVRALREGETIAVYGDFD
                     ADGVTATVLLTEALRAMSDERRLVIPYIPDRVDEGYGLNIDALTSLRTKGVDVVISVD
                     CGIRSPLEAAHARDIGLDMIVTDHHSLGPELPPALAVINPKRDDSTYPERMLAGVGIA
                     FKLAQALRLAMPERANWDETNLLDLVSIGTVADLAPLMGENRKLVAEGLAVLNEVRRP
                     G"
     gene            1073..1312
                     /locus_tag="PK829_06200"
     CDS             1073..1312
                     /locus_tag="PK829_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40842.1"
                     /translation="MSYSWIRASEIGEYVYCRRAWWLRRVRGAASANTAQLQAGTRHH
                     ERHGRLVRRSIWAQRVAYLALFLAVAMLVYRLLGA"
     gene            1338..1871
                     /locus_tag="PK829_06205"
     CDS             1338..1871
                     /locus_tag="PK829_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOU40843.1"
                     /translation="MLLGGLPLLLVLVLLFLAAAFWIGGRRMREESGLPDGRVFYSDT
                     ETWRANNDVLRAARLRLVGKPDYLVEQDDGTIIPVEVKSSRAPERPYEGQVLQLAAYC
                     LLVEENFGVRPPYGILQYRDQAFAIDYSDDLEADLLAVLDEMRAARGESDPDPDHDDP
                     RRCLACGLRAACDRRLA"
     gene            1963..3243
                     /gene="hisS"
                     /locus_tag="PK829_06210"
     CDS             1963..3243
                     /gene="hisS"
                     /locus_tag="PK829_06210"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU40844.1"
                     /translation="MSKQIQRVTGMQDVLPDDRRYWDAIIAAAERVAGRFGFQRVELP
                     IIEAEELFDRGVGQGSDFFLQKEMYTLTEPDGSRLTLRPEFTAGFMRAYIQNGMSNWT
                     QPVKLYTIGPSFRRERPQAGRLRQHSQINCEILGEDDPAADVEVMMVAMSLYRELGYT
                     GLTFQLNSTGCPVCKPGYIKALVAYLDSYRDQLADIDKERLSKNPLRVLDSKEKGMDA
                     LLAGAPRIADHLCDDCRDHFTDVRGLLDELDQTYTINFRLVRGIDYYTKTVFEVWQEG
                     IGAQAALCGGGRYDGLAEDIGGPPTPGVGFGSGVERIVLGMKEQGIAPPVALPVPFYL
                     AHFGGATKTAATQLAFRLRAAGIPARLAFSRRGRSLKSQMREADHLGARYVLIVGEDE
                     LAQGQVTARLLAGGEQTRVPVNDLVAWARARLDE"
     gene            3250..4119
                     /locus_tag="PK829_06215"
     CDS             3250..4119
                     /locus_tag="PK829_06215"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOU40845.1"
                     /translation="MNQVGILQQPRIRESVTLGDEVRRWLTERGVASWTAAPWDDEPI
                     AAPLNETDLVIVLGGDGSTLRAARWTVPFAVPIFGINVGRVGFLSEATPDTWRDKLAR
                     VLAGDFWLERRLLLHAELWRDEGLVQPFMALNEVVIGRGAQARVIHLHLRVDDDLVTT
                     YIADALIVATPTGSTAYAMAAGGPLLPPQLQNFVVVPVAAHLSLNRPLVLHENARIAI
                     QVDMSHEAYLTADGQKSVNVESGDTVVITKHEQPCLFARVESTGFFYRRLMGRLGFSW
                     PHLDPGPERRTQP"
     gene            4230..5111
                     /locus_tag="PK829_06220"
     CDS             4230..5111
                     /locus_tag="PK829_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40846.1"
                     /translation="MEIKEPRLRALLAQAGRNAEYGKNAAAESMYRDILSEAPEAEEA
                     WLGLARVLGDEGAKRAALERALLLSPQNPAAVAGLAELDGRPVPPEAAAALALADEEE
                     AEEPRPGPAGVEPPDPPPARDIDYTLVCYRHPQRMTSLRCYNCNRPICIQCANKTPVG
                     YICPECQRQAEDAFYNAKPSDYLIAAIVSLPISLVVGLLLVSLARGMLFFLLIFFISG
                     AVGSFIGRITKRAIGGRRGRYLPGLVVAMMFIGVLIPVVPLLLAILGGSGGALFALIG
                     PGIYLFVGASAAYWQMR"
     gene            5123..>6233
                     /locus_tag="PK829_06225"
     CDS             5123..>6233
                     /locus_tag="PK829_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU40847.1"
                     /translation="MLSELVIRNFAIIDQLRLEFDPGFNVLTGETGAGKSIILDAVTL
                     VLGERADTTAVRAGSDTAYVEAAFRAAPALQALLRPLLEAEGLDEDGEEFIVLARELR
                     ANGRTICRVNGRTVSLSVLRQVAEPLIDIHGQGEHLSLLRPRAHLPLLDAFGGLDAER
                     EALAAEVRRLHAVERELGELRRSQRDLAQRMEMLQFKAEDIRAAALQPDEEETLRVER
                     LRLANAEQLGRHAAEAVALLTTIDDDRPAVLDLLGRVEHALTQLAKLDDTRAAALETL
                     QGLSFQLNELAGDVQDYLDALEFDPRRLNATEARLELINTLKRKYGETGIPGLLAALE
                     RAETDLKALENSEVRTGELEAERDRYLHVIGGLAGA"
BASE COUNT         1029 a         2106 c         2077 g         1021 t
ORIGIN      
        1 gcccggccgg cgcacctcgt tcaacacggc cagcccctcg gccaccagct tgcggttctc
       61 gcccatcagc ggggccaggt cggccaccgt gccgatggac accaggtcga gcaggttcgt
      121 ctcgtcccag ttggcccgct ccggcatggc caaccgcagc gcctgagcca gcttgaaggc
      181 gatgcccacg ccggccagca tgcgctccgg gtaggtcgaa tcgtcgcgct tgggattgat
      241 gaccgccagg gcggggggga gttccggccc caggctgtga tggtcagtga caatcatgtc
      301 caggccgatg tcgcgggcgt gggccgcctc gagaggcgag cggatgccgc agtcaacgct
      361 gatgacgaca tcgacgcctt tggtccgcag cgaagtcagg gcgtctatgt tcaggccata
      421 cccctcgtcg acgcggtcgg gaatgtaggg gatgaccaga cggcgctcgt cgctcatggc
      481 ccgcagcgcc tcggtcagca acaccgtcgc agtcacgcca tcagcgtcga agtcaccgta
      541 gacagcgatg gtttccccct cgcgcagggc gcgaacgagg cgatcgacgg cgcggtccat
      601 gtcggccaac tggtaggggt cgcgcgagag caggtagtgg tgcgacagaa aggcctgcac
      661 ctgcgcgggc tgggccaggc cgcggttgtg gaggatgcgg gccagaaccg gatgcacggc
      721 atcgccgacc gactgcaagg gcgagtcggc gggcatctcc ggggcaacgc gccagcggta
      781 cgcgcgcaat gattctggcc ggttcataga ggtcaatggt aacacgctca tagggattcc
      841 tcaaagaggc cgcgcgacag ccgtcgggcg gccaatcgat atcagtggca gggcaaacca
      901 catgtgactg agcaagggcg caaacatggc cgatgataag gacgtgggcg gactgtcgcc
      961 caggctgggc ctgccaatct gtcaggttgg cgggcttgtc tcgaccaatc aaaagcccca
     1021 cggcgagcca acgcctcttg actcactgtc tattatccat aaaatagaac gtatgagcta
     1081 ctcctggatt cgggccagtg agattggtga gtatgtctat tgccggcgcg cctggtggct
     1141 gcgccgcgtg cgcggcgcgg cctcggccaa cactgcccag ttgcaagccg gcacacgcca
     1201 ccacgagcgc cacggccgcc tcgtccgccg gtcgatctgg gcgcagcggg tggcctacct
     1261 ggctctgttc ctggccgtgg ccatgctggt ctaccggctc ctgggggcct aggcggccgg
     1321 ggagcgcgag aatcgccatg ttactgggtg gtttgccgct gttgttggta ctggtcttgc
     1381 tcttcctggc cgcggccttt tggatcggcg ggcggcggat gcgcgaggag agcgggctgc
     1441 ccgacggccg cgtgttctat agcgacaccg agacgtggcg ggccaacaac gacgtcctcc
     1501 gtgccgctcg cctgcgtctg gtgggcaagc ccgattacct cgtggagcag gacgacggca
     1561 cgatcatccc cgtcgaggtc aagtcgtcgc gcgcgccgga gcggccctac gaggggcagg
     1621 tcttacaact ggcggcctac tgcctgttgg tcgaagagaa ctttggcgtg cgcccaccct
     1681 atggcattct gcaatatcgc gaccaggcgt tcgccatcga ctacagcgac gacctggagg
     1741 ccgatctgct ggctgtgctc gacgagatgc gcgccgcccg tggcgagagc gaccccgacc
     1801 ccgaccacga cgacccgcga cgctgcctgg cctgtggcct gcgcgccgcc tgcgaccggc
     1861 ggctggcctg atcccctttc actgaatctc cgtttgcggc tataataagc acaatgtcat
     1921 gaatgggccg cccaatgggc gccagcccgt tgtggaggca ctgtgtcgaa acaaatccag
     1981 cgtgtcaccg gcatgcagga tgtcttgccg gacgaccgcc gctattggga cgccatcatc
     2041 gccgcggccg agcgtgtcgc cgggcgcttc ggcttccagc gcgtcgaact gccgatcatc
     2101 gaggctgagg agctgttcga ccgtggcgtg ggccaggggt cggacttctt tctccagaag
     2161 gagatgtaca ccctgaccga gccggacggt tcgcgcctta ccctgcggcc cgaattcaca
     2221 gccggcttca tgcgcgccta tattcagaat ggcatgagca actggacgca gccggtcaag
     2281 ctctacacca tcggccccag cttccggcgc gagcgccccc aggccggccg cctacgccag
     2341 cacagccaga tcaactgcga aatcctgggc gaggacgacc cggccgccga cgtcgaagtc
     2401 atgatggtgg ccatgagcct ctaccgcgag ctgggctaca ccggcctgac cttccagctc
     2461 aacagcaccg gctgccccgt ctgcaagccg ggctacatca aggccctggt agcctacctg
     2521 gatagctacc gcgaccagtt ggccgacatc gacaaggagc ggctaagcaa gaacccgctg
     2581 cgcgtcctcg acagcaaaga gaagggcatg gacgcgctgc tggctggcgc gccgcgcatt
     2641 gccgaccacc tctgcgacga ctgccgcgac cacttcaccg acgtgcgcgg tctgctggat
     2701 gagctagatc agacctacac catcaacttc cgcctggtgc gcggcatcga ctattacacc
     2761 aagaccgtct tcgaggtctg gcaagagggc attggcgcgc aggcggcgct atgtggtggc
     2821 ggccgctacg acgggttggc cgaggacatc ggcgggccgc ccacgcccgg cgtcggcttc
     2881 ggcagcggcg tcgagcgcat cgtcctgggc atgaaggagc agggcattgc ccccccggtg
     2941 gctttgcccg tgcccttcta cctggcccac tttggcgggg cgacgaagac agcggcgacg
     3001 caactggcct tccgcctccg ggcggcgggc attccggcgc ggctggcctt ctcgcgccgc
     3061 gggcgcagcc ttaagagcca gatgcgcgag gccgaccacc tcggcgcgcg ctacgtcctc
     3121 atcgttggcg aagacgagct ggcccagggg caggtgacgg cgcggctgct ggccggcggg
     3181 gagcaaacgc gcgtgcccgt taacgatctg gtcgcctggg cgcgggcgcg gctggacgag
     3241 taagggccta tgaaccaggt tggtatcctc cagcagcccc gcatccgcga atcagtcacc
     3301 ctgggcgacg aagtgcgccg ttggctgacc gagcgtggtg tggcctcgtg gacggccgcg
     3361 ccctgggacg acgaacccat cgccgcgcca ctgaacgaga ccgacctggt catcgtcctg
     3421 ggcggcgacg gctcgacgtt gcgggcggcg cgctggacgg tgcccttcgc ggtgcctatc
     3481 ttcggcatca acgtcggtcg tgtcggcttt ctgagcgaag ccacgcccga cacctggcgc
     3541 gacaagctgg cgcgcgtgct ggccggcgac ttctggctgg agcgccgcct actgctgcac
     3601 gccgagttgt ggcgcgacga gggtttggtg cagccgttca tggccctcaa cgaagtcgtc
     3661 atcggccggg gggcgcaggc gcgcgtcatt cacctccatc tgcgcgtcga cgacgacctg
     3721 gtgacgacct acatcgccga cgccctcatc gtggccacgc cgaccggctc aacggcctac
     3781 gccatggccg ccggcgggcc gctgctgccg ccgcaattgc agaacttcgt cgtcgtgccc
     3841 gtggccgctc acctgagcct gaaccggccg ctggtgctgc acgaaaacgc ccgcatcgcc
     3901 attcaggtcg atatgagcca cgaggcctac ctgaccgccg acggccagaa gagcgtcaac
     3961 gtagagagcg gcgacacggt ggtgatcacc aagcacgagc agccctgtct cttcgcgcgc
     4021 gtggagagca cgggcttctt ctaccgccgc ctgatgggcc ggctgggctt tagctggccc
     4081 cacctggacc ccggcccaga gcgacggacg cagccctagt cagcccacat ccttagttgc
     4141 cgctcatctg cttcttgtcc gttgtttatg aagccgggcg acaatccagc ccaacggcat
     4201 cgagcgaaca ctgctggagt aaggcacgca tggagataaa ggaaccacga ttgcgcgcgc
     4261 tgctggccca ggccggccgc aatgctgaat atggcaagaa tgccgccgcc gaatcgatgt
     4321 accgcgacat cctgagcgaa gcgccggagg ccgaggaggc ttggctgggt ttggcgcgcg
     4381 tgttgggcga cgagggcgcg aagcgcgccg cccttgaacg tgcgctgttg ctctcgccgc
     4441 agaacccggc cgcggttgcc gggctggcgg aactggacgg ccggcccgtg ccgccagagg
     4501 ccgccgcggc cctggccctg gccgacgagg aagaggctga ggaaccgcgc cccggccctg
     4561 ctggtgtcga gccgccagac cctccccccg ctcgcgacat cgactatacc ctggtctgct
     4621 atcgccaccc gcagcgcatg acctccctgc gctgttataa ctgcaaccgg ccgatctgca
     4681 tccagtgcgc caacaagaca cccgtcggtt acatctgccc ggagtgccag cgccaggccg
     4741 aagatgcctt ctataacgcc aaaccgagcg attacctgat cgccgccatc gtgtcgctgc
     4801 ccatcagtct ggtcgtcggc ctgctcctcg tcagcctggc ccgcggcatg ctgtttttcc
     4861 tgctcatctt cttcattagc ggcgcggtcg gcagcttcat tggccggatc accaagcggg
     4921 ctatcggcgg acggcgcggg cgctacctgc ccggccttgt ggtggccatg atgttcatcg
     4981 gcgtgctcat tccggtggtg cccctgctgc tggctatcct gggcggcagc ggcggcgcgc
     5041 tgttcgccct catcggccca ggcatctatc tgttcgtcgg ggccagtgcc gcctactggc
     5101 aaatgcgcta aaatagcgac gtatgctatc ggaacttgtc atccgtaact tcgctattat
     5161 cgatcagttg cggctcgaat tcgatcccgg cttcaacgtc ctgaccggcg agacgggcgc
     5221 gggcaagtcg attatcctcg acgcggtgac gctggtgctg ggcgagcgcg ccgacacaac
     5281 ggccgtccgc gccggcagcg acaccgccta cgttgaggcc gccttccgcg ccgctccggc
     5341 gctgcaagcg cttctgcgcc ccctgctgga ggccgaaggg ttagacgagg acggtgagga
     5401 gttcatcgtg ctggcgcgcg agttacgcgc caacggccgc accatttgcc gcgtcaacgg
     5461 tcgcacggtc agcctatcgg tattgcgtca ggtggccgaa ccgcttatcg acatccacgg
     5521 ccagggtgag catctgtcgc tgttgcgtcc gcgcgcccac ttgcccctgc tcgatgcctt
     5581 tggcgggctg gacgccgagc gcgaggcgct ggccgccgaa gtgcgccggc tccacgccgt
     5641 tgagcgcgag ttgggcgagc tgcgccgcag ccagcgcgac ctggcccagc gcatggagat
     5701 gctgcaattc aaggccgagg acatccgcgc cgccgccttg cagccggacg aagaggagac
     5761 gttgcgcgtc gagcgcctgc ggttggccaa cgccgagcaa ctgggccgcc acgccgccga
     5821 ggccgtggcc ctgctgacga ccatcgacga cgaccgcccg gccgtgctgg acctgctcgg
     5881 ccgggtcgag cacgccctga cgcaactggc caagctggac gacacccgcg ccgcggcgct
     5941 ggagaccctg caagggctga gtttccagct caatgagctg gccggcgacg tgcaggacta
     6001 cctcgacgcg ctggagttcg acccccgccg gctgaacgcc accgaggcgc ggctggaact
     6061 gatcaacacc ctcaagcgca agtacggcga gaccggcatc cccgggctgc tggccgcgct
     6121 ggagcgcgcc gaaaccgacc tgaaggcgct ggagaacagc gaggtgcgca cgggcgaact
     6181 ggaggccgag cgcgaccgct acctgcacgt catcggtgga ctggccgggg cgc
//
LOCUS       DAOSMQ010000143         6216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12705_length_6216_cov_4.906346, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000143 DAOSMQ010000000
VERSION     DAOSMQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12705_length_6216_cov_4.906346"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..595)
                     /locus_tag="PK829_06230"
     CDS             complement(152..595)
                     /locus_tag="PK829_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40848.1"
                     /translation="MSNVQLPATTQPDTHKEPLIPIGYVIAGVIVTLVISVAFIVLMV
                     YLANNYADTILVVRDIFIIALGLMSCLSGIVLTLLLIAVIRLVNMLEFELKPILMKTN
                     ETLGTIRGTTVFMSENVVRPMTAASSYVAGLRRGVATLFGNPRRR"
     gene            complement(643..927)
                     /locus_tag="PK829_06235"
     CDS             complement(643..927)
                     /locus_tag="PK829_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40849.1"
                     /translation="MIAVPGSASGLVLGFLLASAYGAIFHLIYGGSLRRILLYLAAAW
                     VGFIIGQLAGDFLNLEWFKVGKLHLLSASVGAWLLLLGARWLAGQETPRE"
     gene            complement(924..2105)
                     /gene="mnmA"
                     /locus_tag="PK829_06240"
     CDS             complement(924..2105)
                     /gene="mnmA"
                     /locus_tag="PK829_06240"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOU40850.1"
                     /translation="MNATLCALPDATVVPAPLTGSQPRVVVAMSGGVDSSVAAALLVE
                     AGYEVTGIMMRLWSEPAEGATAPANRCCTPDQMTDARRVADRLGIPFYVIDVRDHFRR
                     AIVQFFLDEHAAGRTPNPCIECNRQIRFTYLLERALALGADYLATGHYARVRHDADGY
                     RLLRGLDGDKDQSYVLHMLGQTHLARVLFPVGAYTKAQVRELARAHNLPVANKHDSQD
                     LCFLKDGDYRGFLRRHSEGISRPGDIVTVDGRVIGQHGGLVDYTIGQRKGLGIAAPEP
                     LYVVGKDAARNALIVGPRDALGRDRLAARAVSWVGAPPPAPIAAAVKIRYKAAPLPAT
                     IAPGVDGTAMVQLDEPAYGVTPGQAIVFYDGDVCLGGGLIAGEGWPGPTLEPAVGSSA
                     Q"
     gene            complement(2119..3318)
                     /locus_tag="PK829_06245"
     CDS             complement(2119..3318)
                     /locus_tag="PK829_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOU40851.1"
                     /translation="MTRGLQPNPIYLDHGATTPVLPAVVEAMAPYWGEHYGNPSSLHR
                     TGAVAARAVESARETFAELLGVEPGAVVFTACGSESNNLALRGVLFAAREAGRGHHLI
                     TSAIEHSAVLATARQLRDHFGFELTVLPVDEFGRVRPEDVAAAVRPETALISVMAANN
                     EIGTIQPWAEIGALARERGILFHTDAVQLAATRHWNLAVEPIDLMSLGPHKFYGPKGV
                     GVLVVRPGVELLPTLTGGGHEGGLRAGTTNVPLVVGAAAAFQLAMTERDERLAHVRRL
                     RDRLLAELPAALPGRCLITGHPTERLPHHASFALRGLSGNDLLIHLDQAGIAASSGSA
                     CASGDPKPSAILQALGLGPEWTLGGLRLTLGRQNSDADIDAVAAALPAIVARLDAIEK
                     RFAVAHA"
     gene            complement(3321..5396)
                     /locus_tag="PK829_06250"
     CDS             complement(3321..5396)
                     /locus_tag="PK829_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU40852.1"
                     /translation="MSDPPQTMDELAPLWRDILGDLRDVFDVHFVCATFAYHLARHTR
                     TRALVAVADSQAKCYDVWLTEPSGRATQMRWLIDEAGLESLLTLDGPAAIDKLERPAA
                     EIIGGQVWLLVKRRLLAAPLPRQQRLYNAFPPALLLLLDPEGPAELEPLLREMTSLMC
                     VFLDRAALRQEVDRRTVEFAVISDISRALSSTLNIQQIYELLTGPIRQTLNVETLSIG
                     LIEPVTGDILFVQRLFGPMFEQLPPVRLKRGQGIAGWVAEHKEPVIITDAYADQRFFS
                     GVDTRSGFRTHSMICIPLQVDERTIGILEAINRQFGRFSEHDLQLMQALGGPLAAAIE
                     NANLHADVLSEKRRIETLFTSMSDGLVTVNREGMITRINGAFASMLYSDDDAIVGQPL
                     HERVHTKQGDIQKLVGRLLSGAEDSVQLAADIVRDDGRVLPVLISGAPVDDETGAAQE
                     AIFVFSDLTQIREVERMRDDLFHGIIHELRTPLATILIYSRLLREGRAQAERAERFLG
                     VIERESDRLQRMVRQMLDLAKLEAREMQRSAEPVWLNPLFDETLPPLAERAVQKGLLF
                     RQQIENDLPPVLGNQDIYDMVLKNLVDNAIKFTPSGSIQVTARRDNGVVIIEVSDEGI
                     GIPAEAMNNLFKRFYRTQTAVERGIAGTGLGLYMVKENLRNYNGHIAVQSVLGKGTTF
                     TIRLPVAQM"
     gene            5549..>6216
                     /gene="eno"
                     /locus_tag="PK829_06255"
     CDS             5549..>6216
                     /gene="eno"
                     /locus_tag="PK829_06255"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123706.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU40853.1"
                     /translation="MDYIESVLAREVLDSRGNPTVEVEVALYDGAKGRAMVPSGASTG
                     VHEAWEKRDGDKKRYGGKGVLGAVEAVNEEIADAIVGWTATDQIGIDQAMIELDGTEN
                     KSRLGANAILGVSLAVAHAAAASQDLPLYRYLGGVGGRTLPVPMMNIMNGGKHAAGAT
                     DMQEFMVMPVGATSFREGLRWGVEIYQSLKKVLGKKGYPTTVGDEGGFAPRVSANSEA
                     FDLI"
BASE COUNT         1105 a         1934 c         2141 g         1036 t
ORIGIN      
        1 gaagtgcagg agttggtcgc gggtcgcctg gcccgattgg ggcgcgagga tgatggcggt
       61 ggccgcgccc accagcgagc cgatcacaaa accggccagg aaggcgccca gatcgctgtc
      121 gttgtcgctc atttcatttc tcctgaagtc gctatctgcg ccgtgggttg ccgaaaagcg
      181 tagccacgcc ccgccggagg ccggccacgt agctgctggc cgcggtcatg gggcgcacaa
      241 cgttttcgct cataaagacc gtcgtgcccc ggatggtgcc cagggtctca ttggtcttca
      301 tcaaaatcgg tttcaactcg aactcaagca tattgaccag acggatgacg gcaatgagta
      361 atagggtcag cacgatgcca gacaggcaag acatcaggcc gagggcgatg atgaagatat
      421 cgcgcacgac cagaatggtg tcggcgtagt tgttggccag atagaccatc aggacgataa
      481 aggcaacaga aatcactaga gtgacgatca ctccggcgat gacgtagccg atcggaatga
      541 gcggctcttt gtgggtatcc ggctgtgttg tggccggcag ttgtacattg ctcatcgcat
      601 gaaccccaac tcaaaagtat agcacgaatc ggccggcgcg tctcattctc gtggcgtttc
      661 ctgcccggcc agccagcgcg cgcccagcag tagcagccaa gcgcccacgc tggcgctcag
      721 caggtggagc ttacccacct tgaaccactc caggttgaga aagtcgccgg ccagctggcc
      781 gatgataaag cccacccagg ccgcggccag atagagcaag atgcgccgca gggagccacc
      841 gtagatgagg tggaaaatgg ccccgtaagc actggctaac agaaagccga gcaccaaacc
      901 ggaggccgag ccgggaacgg cgatcactgc gcgctactcc ccacagccgg ttcgagcgtt
      961 ggccccggcc acccttcccc ggcgatcagg ccgccgccga ggcagacgtc gccgtcatag
     1021 aagacaatcg cctgaccggg cgtgacgcca taggccggct cgtccagttg caccatcgcc
     1081 gtgccgtcca cgccgggggc gatggttgcc ggcaagggcg cagctttgta gcgaatcttg
     1141 accgcggcgg cgatcggcgc ggggggcggc gcgccgaccc agctgacggc gcgggcggcc
     1201 agccggtcgc ggccgagcgc gtcgcgcggc ccgacgatga gggcgttgcg cgccgcgtcc
     1261 ttgccgacga cgtagagcgg ctccggcgcg gcgatgccca gccccttgcg ctggccgatg
     1321 gtgtaatcga ccaggccgcc atgttggccg atgacgcggc cgtccacagt gacgatgtcg
     1381 ccggggcgcg agatgccctc gctgtggcgg cgcaggaaac cgcgatagtc gccgtccttc
     1441 aggaagcaga ggtcctggct gtcgtgcttg ttggccacgg gcaggttgtg ggcgcgggcc
     1501 aattcgcgca cctgggcctt ggtatatgcg ccgacgggga agagcacgcg ggccaggtgc
     1561 gtctggccga gcatgtgcag gacgtaggac tggtccttat cgccgtccag gccgcgcagc
     1621 agacggtagc cgtcagcgtc atgccggacg cgggcgtaat ggcccgtggc caggtagtcg
     1681 gctcccaacg ccagcgctcg ctccagcagg taggtgaagc gaatctgccg gttgcactca
     1741 atgcaggggt tgggcgtgcg cccggcggcg tgttcgtcca ggaagaactg gacgatggcg
     1801 cggcggaaat gatcgcgcac gtcgatgacg tagaagggga tgcccagccg gtcggccacg
     1861 cggcgggcgt cggtcatttg gtcgggcgtg cagcagcggt tggccggggc ggtggccccc
     1921 tcggccggct cgctccacag gcgcatcatg atgcccgtta cttcgtaacc agcctcaacc
     1981 agcagcgccg cggctacgga gctatcaacg ccaccgctca tggcgacgac gacgcgcggt
     2041 tggctgcccg tcaggggcgc agggacgacg gtggcgtcgg gcagggcgca gagtgtggcg
     2101 ttcatgagca tggctggttc aagcgtgggc aacggcaaag cgcttctcga tggcgtccag
     2161 ccgggccaca atcgccggca gggcggcggc gacggcgtcg atgtcggcgt cgctgttctg
     2221 gcggccgagc gtcagccgca agccgcccag cgtccactct gggccgaggc cgagggcctg
     2281 gaggatggcc gaaggcttgg ggtcgccact ggcgcaggct gagccgctgc tggcggcgat
     2341 gccggcctgg tcgagatgga tcaacaggtc gttgccgctc aggccgcgca gggcgaagct
     2401 ggcgtggtgg ggcagccgct cggttgggtg gccggtgatg agacagcggc cgggcagagc
     2461 cgccggcaac tcggccagca gccggtcgcg caggcgccgc acatgggcca ggcgctcgtc
     2521 ccgttcggtc atggccaatt ggaaggccgc ggccgcgccg acgacgaggg gcacgttggt
     2581 tgtgcccgcg cgcaggccgc cctcgtggcc gccgccggtc agcgtgggca gcagctcgac
     2641 accgggacgc acgaccagca cacccacgcc cttcggcccg tagaacttat gcgggccaag
     2701 cgacatcagg tcgatgggtt cgacggccag attccagtgg cgcgtggcgg ccagctgtac
     2761 ggcgtcagtg tggaagagga tgccgcgctc gcgggccagc gcgccgatct ccgcccaggg
     2821 ctggatcgtg ccgatctcgt tgttcgcggc catgacgctg ataagcgccg tctccgggcg
     2881 gacggccgcg gcgacatcct ccgggcgaac gcggccgaat tcgtccacgg gcagcacggt
     2941 cagttcgaag ccaaagtgat cgcgcagttg ccgggcagtg gccagcacgg cgctatgctc
     3001 gatggcgctg gtgatcaggt ggtggccgcg gccggcctcg cgggcggcga acagcacccc
     3061 gcgcagggcc aggttattgc tctctgagcc gcaggcagtg aagacgacag cccccggctc
     3121 gacgcccagc agttcggcga acgtctcacg ggcggactcg accgcgcgcg cggcgacggc
     3181 cccggtgcgg tggagggacg acgggttgcc gtagtgctcg ccccagtagg gggccattgc
     3241 ctcgacgacg gcgggaagca ccggcgtcgt cgcgccgtgg tccaggtaga tggggttggg
     3301 ttggagtcct ctggtcatga tcacatctgt gccacgggca gacgaatggt gaacgtcgtg
     3361 cccttgccca atacgctttg gacggcaatg tgcccattat agttgcgcag gttctctttg
     3421 accatgtaga ggcccaaccc cgtgccagcg ataccccgct ccacggctgt ctgggtgcga
     3481 tagaagcgct tgaagaggtt gttcatggcc tcggcgggga tgccgatacc ctcgtcgctg
     3541 acctcaatga tcacgacgcc gttgtcgcgc cgggccgtca cctggatact gcctgacggc
     3601 gtaaacttga tagcgttgtc gaccaggttc ttcagcacca tgtcgtaaat gtcctggttg
     3661 cccagcaccg gcggcagatc gttctcgatc tgttgccgga agagcagtcc cttctgcaca
     3721 gcgcgttcgg ccagtggcgg cagcgtttcg tcaaagagcg ggttcagcca caccggttcg
     3781 gcgctgcgct gcatctcgcg cgcctccaac ttggccagat cgagcatctg gcgcaccata
     3841 cgttgcaagc gatcgctctc ccgttcgatg acgcccagaa agcgctcggc ccgctcggcc
     3901 tgcgcccggc cttcgcgcag caagcgggag tagataagga tggtcgccag cggcgtacgc
     3961 aactcgtgga tgatgccgtg gaaaaggtcg tcgcgcatcc gttcgacctc gcgaatctgc
     4021 gtcaggtcgc tgaagacgaa aattgcctcc tgggccgcgc ccgtttcgtc gtctaccggc
     4081 gcgccgctga tgaggacggg caagacgcgg ccgtcgtcgc gcacaatgtc agccgccaac
     4141 tgcacggagt cctccgcgcc cgatagcaac cggccgacga gcttctggat gtcgccctgc
     4201 ttggtgtgca cacgctcgtg taggggctgg ccgacgatag cgtcgtcatc gctgtagagc
     4261 atggaggcga acgcgccatt gatgcgcgtg atcatgccct cgcgattgac ggtaactagg
     4321 ccgtcggaca tgctggtaaa gagcgtttcg atgcggcgct tttcggacag cacgtcggcg
     4381 tggaggttgg cgttttcgat ggccgcggcc aacggcccgc ccagcgcctg catcagttgt
     4441 aggtcgtgct cgctgaagcg gccaaactgg cgattgatgg cctcaaggat gccgatggtg
     4501 cgctcatcaa cttgcagggg aatacagatc atcgagtggg tgcggaaacc ggagcgcgta
     4561 tcgacgccgg agaagaagcg ctggtcggcg taggcgtcgg tgatgatgac tggctctttg
     4621 tgctcggcca cccagccggc aatgccctga ccgcgcttca gccgcaccgg cggcaattgt
     4681 tcaaacatcg gcccgaagag ccgctgcaca aacagaatat cgcccgtcac cggctcgatc
     4741 aggccgatgg atagcgtctc gacgttgagc gtctggcgga tggggccggt cagcaactcg
     4801 tagatttgct gaatattgag cgtggagctg agggcccggc tgatgtcgct gatgaccgcg
     4861 aattcaaccg tgcgccggtc gacttcctgg cgcagggcgg cgcggtcgag gaagacgcac
     4921 atcaggctgg tcatctcgcg cagcagcggc tccagctcgg ccggcccttc ggggtcgagt
     4981 agcagcagca gggcgggcgg aaaggcatta tacagccgct gctggcgggg aaggggcgcg
     5041 gccaggaggc ggcgtttgac cagcagccag acctgcccac cgatgatctc ggccgccggc
     5101 cgctccagct tgtcgatcgc tgccggcccg tcaagggtca acaatgactc cagcccggcc
     5161 tcatcgatca gccagcgcat ttgcgtcgct cgccccgaag gctctgtcag ccagacgtcg
     5221 tagcacttgg cctggctgtc ggccacggct actagtgcgc gcgtgcgggt gtggcgggcc
     5281 agatgatagg caaaggtggc gcagacaaaa tggacatcga acacatcacg caaatccccc
     5341 aggatgtcgc gccagagagg ggccaactcg tccattgtct ggggcgggtc gctcatagcg
     5401 gggcagtgta acgccgccct ctccgcgaag caagccaatc ccgccgacac tcatagccgc
     5461 gcgtcgcttg ccgaccgcca ccgctttcag tatagttagc cgtaccgcac ccgctattca
     5521 gcgtgtttac caaccaagga gattcacaat ggactacatt gaatcagtgc tcgcccgcga
     5581 ggttcttgat tcgcgcggca atcccactgt tgaagttgag gtcgccctat acgacggcgc
     5641 taagggacgg gccatggttc cctccggcgc gtccacaggc gtgcatgaag cctgggagaa
     5701 gcgcgacggc gacaagaagc gctacggggg caaaggcgtc ctcggcgctg tggaagcggt
     5761 caacgaagag atcgctgatg ccattgtcgg ctggacggcg accgaccaga ttggcatcga
     5821 ccaagccatg attgagttgg acggcacaga gaacaagagc cgcctgggcg cgaacgcgat
     5881 cctgggcgtc tcgctggccg tggcccacgc cgccgccgcc agtcaggact tgccccttta
     5941 tcgctacctc ggcggcgtcg gcggccgcac gctacccgta ccgatgatga acatcatgaa
     6001 cggcggcaag cacgctgccg gcgcgacgga catgcaggag ttcatggtca tgcccgttgg
     6061 ggcgacgtca tttcgcgagg gtttgcgctg gggcgtagag atttaccaga gtctgaagaa
     6121 ggtgctgggc aagaagggtt atcctacgac tgtgggcgac gagggcggct ttgccccgcg
     6181 cgtctcggcc aacagcgagg cattcgacct gatcat
//
LOCUS       DAOSMQ010000144         6161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12839_length_6161_cov_5.225352, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000144 DAOSMQ010000000
VERSION     DAOSMQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12839_length_6161_cov_5.225352"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PK829_06260"
     CDS             <1..600
                     /locus_tag="PK829_06260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU40854.1"
                     /translation="DAQVVVGRLVNQPLRIRLPVTATAYDTQYVTVGEALDGLLDPAL
                     RATEDEFLRGIYAVFDETQRWEGVFQYPVTTTVISAGYGGRRSYNNGPIEIYHTGLDI
                     AAAQGDTVSAPANGVVVFSDQLELRGGTLILNHGRGVMTGYYHLSARLVNVGDEVVAG
                     QPIARVGSTGLSSGAHLHWDLRIMDVPVDPLAWTQQTFP"
     gene            684..2243
                     /locus_tag="PK829_06265"
     CDS             684..2243
                     /locus_tag="PK829_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40855.1"
                     /translation="MTQTQIQSAEYWESGFSLSDTDFEQIYNHFLETEKPQTVAELAG
                     LVIRARVREQAAEVRRLMSGSTVYQPQRSYTVGEEVIFPSLKLARGKVTGIRPGYNPE
                     YGDFNVIVVAIKGKTREFVADYGPAHALNSSNGGALTEEMAGDAQAILDAHGATVAAR
                     IAAALARREDFINIGDEWFVKSLMADVNIGHLHLTEAVLDMAEGGPMTTTEILPQLEL
                     DGGVDARVQEFSLNYHLQRDGRFDDVAAVGAPHWFLRRMEPESVQTTPERLVYQPVVY
                     DRGLLSPQQIALEAELDDEWSDLPPAEPAEPIVWALSYPHRWAGTLPINARTRALFDA
                     GRSPRRRIVFIDDESNESIPGWVVAEGRYVLGLVGWYRDNAIPVGGFIHLRPGSEPGT
                     VLLGYDRRRPQREWVRLAAVKDNKLQFELMRRSIPCGYDDLLIVGTDVVAAVDAHARR
                     ATSMGMSVGTLLSEVFPSLAGLTPQNSVHAKTLYSAINMLRRVPPGPIFAELMRNSSF
                     KAVGDHYWQHE"
     gene            2281..2730
                     /locus_tag="PK829_06270"
     CDS             2281..2730
                     /locus_tag="PK829_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40856.1"
                     /translation="MSSYPRPTIKTAHLRRVTPDTRFFIDYKWWEEGHLDLRTYLLAR
                     LSLGIEGGAELPAERVDLVDAKTGEVRQVDAFQYLVQNYFSRHGNELALQGSIVDAVF
                     SVLLANGNQPMAIREIAERVQRPSDLLVKTFGGPQIYNGIRPLFDDE"
     gene            2988..3061
                     /locus_tag="PK829_06275"
     tRNA            2988..3061
                     /locus_tag="PK829_06275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3022..3024,aa:Arg,seq:tct)
     gene            3093..4766
                     /locus_tag="PK829_06280"
     CDS             3093..4766
                     /locus_tag="PK829_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOU40857.1"
                     /translation="MTVKIFTVAEMVAAEKAADAAGHSYAEMMEAAGRSVADAIQQRR
                     DVADCQVLVLVGPGNNGGDGLVAGRYLAEAGAVVAFYLVKPRDVADDENLARAEEMGL
                     EVLLADYDQRYRVLRHRLRITDIVIDALLGTGVSRAVEGQLAALLEQAGGAVAERRRA
                     APARAPLLSVAAPPLAPEDTPAGDERSRQPFVVAVDCPSGLNCDSGALDPLAIPADLT
                     VTFAGPKRGHFRFPGASACGELVVADIDIDPKLPAVAGVPLELATAEGARALLPARPP
                     DGHKGTFGTALIAAGSIRYWGAPALAARGAFRGGAGMVTLAVPQRLRPALAGQFPEAT
                     YPAVTDTDTLGVATANLLLGVLDRTGALLVGPGLGEAASFLSALLDGLRSPAEGVAVP
                     PLIVDADGLNLLAQMPDWPGRLPANTILTPHPGEMARLVGRPLPALRDEDRVELARDR
                     AAAWGHVVLLKGAYTVIAAPDGRSTLLPFANPVLAVAGSGDVLGGVIVGLLAQGVEPY
                     DAAVLGGYLHGAAGQLAAGYWGNAGLLASELADWVSHVRRALSSRPASA"
     gene            4922..5398
                     /locus_tag="PK829_06285"
     CDS             4922..5398
                     /locus_tag="PK829_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOU40858.1"
                     /translation="MTIRHIIDYPPRTSSVQNIFRLLLGAALLYAGISHLTFARTEFM
                     AQVPSWLPLDGDLVVVLSGIVEILLGVSLIVLRRYRALVGWVAAIFFVVIFPGNISQY
                     VNGIDAFGLNTDTARFVRLLFQPLLVLWALWSTGAWQAWRSRHQNAPQRPVSETGR"
     gene            5466..5927
                     /locus_tag="PK829_06290"
     CDS             5466..5927
                     /locus_tag="PK829_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU40859.1"
                     /translation="MAYEQLKLENQICFPIYAASRLITREYQPLLDELGLTYPQYLAL
                     MVLWEHNGLSVNDIGQKLLLNTNTVTPLLKRMEAQGLVTRQRSADDERRVIVSLTPQG
                     QALQERAAAIPPQLAAGLNADGVDEQALQAMLAQLQKIIQHLSAKQSGLSE"
BASE COUNT         1031 a         1989 c         2027 g         1114 t
ORIGIN      
        1 gacgcccagg tggtggtggg gcggctggtc aatcagccgc tgcgcatccg cctgccagtg
       61 acggcaacgg cctacgacac gcagtacgtg accgtcggcg aggcgctgga cggcctgctc
      121 gacccggccc tccgggcgac ggaagacgag ttcctgcgcg gcatctatgc cgttttcgac
      181 gagacgcagc gctgggaggg ggtcttccag tatccagtga cgacgacggt catctcggcc
      241 ggctacggcg gccgccgctc ttacaacaac ggccccatcg aaatctacca taccggcctc
      301 gacatcgccg cggcgcaggg cgacacggtc agcgccccgg ccaacggtgt cgtggttttc
      361 agcgaccaac tggaattgcg tggcggcacg ctcatcctca accacggtcg cggcgtcatg
      421 accggctact atcatctgtc cgcgcggttg gtcaacgtcg gcgatgaggt tgtcgccggt
      481 cagcccattg cccgcgtcgg ctcgaccggc ctttccagcg gcgcgcacct gcattgggac
      541 ttgcggatca tggacgtccc cgtcgatcca ctggcctgga cacagcagac atttccttaa
      601 ggttgtctga gaccgcgcgg cctggtacaa tggtaaacct tagaccactc cacctagact
      661 aggctgccct acaggagttc gcggtgactc agactcagat tcagtctgcg gagtactggg
      721 aatcggggtt ctcactctcc gatactgact tcgagcaaat ctacaatcac tttttagaaa
      781 cagaaaagcc ccagaccgtt gccgagctgg ccggccttgt cattcgcgcg cgcgtgcgcg
      841 agcaggcggc tgaggtgcgg cggctgatga gcggcagcac cgtctatcag ccccagcgca
      901 gctacaccgt cggcgaggag gtcatcttcc cgtcgctgaa gctggcccgg ggcaaggtga
      961 cgggcatccg gccgggctat aaccctgagt atggcgactt taacgtcatt gtcgtcgcca
     1021 tcaagggtaa gacgcgcgag ttcgtggccg actatggccc cgcccacgcg ctgaatagca
     1081 gcaacggcgg tgctctgacc gaggaaatgg ccggcgacgc gcaggcgatc cttgacgccc
     1141 atggggccac cgtggccgcg cgcatcgccg cggccctggc caggcgcgag gacttcatca
     1201 acatcggcga cgagtggttc gtcaagtcgc tgatggccga cgtcaacatt ggccacctgc
     1261 acctgaccga ggctgtgctc gacatggccg agggtgggcc gatgaccacg accgagatct
     1321 tgccccagct agagttagac ggtggcgtgg acgcgcgcgt gcaggaattc tcgctgaact
     1381 accacttgca gcgcgacggc cgcttcgacg atgtcgccgc ggtgggcgcg ccacactggt
     1441 tcctgcgccg catggagccg gagagtgtgc agacgacgcc ggagcgattg gtctaccagc
     1501 ccgttgtcta cgaccggggc ctcctctcgc cgcaacagat cgccctggag gcggagctag
     1561 acgacgaatg gtcggacttg ccgccagccg agccggccga gccgattgtt tgggcgctgt
     1621 cttaccccca ccgttgggcg ggcacgctgc ccatcaacgc gcgcacgcgg gcactgtttg
     1681 atgccggccg ttcgccgcgc cggcgcattg tcttcatcga cgatgagagc aacgagtcga
     1741 ttcccggttg ggtggtggcc gaggggcgtt atgttctggg cctggtgggc tggtatcgcg
     1801 acaacgccat tcccgtgggc ggctttatcc atctgcgccc tggttcagag ccgggcacag
     1861 tcctgctggg ctacgaccgg cgacggccgc agcgcgaatg ggtgcggctg gcggcggtga
     1921 aagataacaa actccagttc gagctgatgc gccggtcgat tccctgcggc tacgatgacc
     1981 tgctcatcgt tggcacagac gtggttgccg cggttgacgc ccatgcgcgg cgggcgacgt
     2041 cgatggggat gtcggtcggc acgctgctga gcgaggtctt tccgtcgttg gccggcctga
     2101 cgccgcagaa cagtgtccac gccaagacgc tttacagcgc catcaatatg ttgcggcgcg
     2161 tgccgccggg gccgatcttc gccgaactga tgcgcaattc gtcgtttaag gccgttggcg
     2221 accattactg gcagcacgag taggcgctcg gctaccgtta ttcataggag atagatagcg
     2281 ttgtccagct atccacggcc aacaatcaag acggcgcacc tgcgacgggt gacgccggac
     2341 acgcgcttct tcatcgacta caaatggtgg gaagagggcc acctcgacct gcgtacctac
     2401 ctgctggccc ggctgtcgct gggcatcgaa ggcggggcgg agttgcccgc ggaacgcgtc
     2461 gacctggtcg acgccaagac gggcgaagtg cggcaggttg acgccttcca atatctcgtc
     2521 cagaactact tcagccgcca cggcaatgag ttggccctgc aaggctcaat cgttgatgct
     2581 gtcttcagcg tcctgctggc caatggcaac cagccgatgg cgatccgcga gatcgctgag
     2641 cgcgtccaac ggccgagcga ccttctggtg aagacgttcg gcggcccgca gatctacaac
     2701 ggcatccggc cgctgttcga tgatgaatag gatcgaaacc ctcctggccc aactaggtgt
     2761 tcccgatggc ctggccagcg ccgccacgcg gaacgatgag gctgtggagg caggccaacc
     2821 cttgcgcgag ggcgatcgcg tcactctctt tcccccgcgg ccggcggttg acgcttctcc
     2881 cgtcccactc tagaattgca gcgcatgggt gggtctcagg ccactctgct ggaatcatcc
     2941 gcgcgtcctg agacagggcg cggcctgccc tgggcaagtg acaaagcgcc cccgtagctc
     3001 aataggacag agcgtcggcc ttctaagccg aatgttgcag gttcgagccc tgccgggggc
     3061 actggcgacg aaccacacag accaggatca ccatgactgt caaaatcttc actgtggccg
     3121 agatggtcgc ggccgagaag gcggccgacg ccgccggcca ttcctatgcc gaaatgatgg
     3181 aggcggccgg tcgcagcgtg gccgatgcca ttcagcagcg ccgcgacgtg gccgattgcc
     3241 aggtactggt gctggtgggg ccgggcaata atggcggcga cgggctggtg gccggtcgct
     3301 atctggccga ggccggggcg gtggtggctt tctacctggt caagccgcgc gacgtagccg
     3361 acgatgaaaa cctcgcccgc gccgaagaga tggggctaga ggtgctgctg gccgactacg
     3421 atcagcgcta tcgggttctg cgccaccgtc tacgcattac cgacatcgtc attgacgccc
     3481 tgctgggcac gggcgtctcg cgggccgtcg agggccaact ggcggcattg ctggagcaag
     3541 ccggcggagc tgtggccgag cgtcgccgcg ccgcgccggc gcgcgcgccg ctgctcagcg
     3601 tcgccgcgcc gcccctcgcg ccggaggaca cgccggcagg cgacgagcgt tcgcgacagc
     3661 cgtttgttgt cgccgtagac tgccccagtg ggctgaactg cgacagtggc gcgctcgatc
     3721 cgctggccat cccagccgat ctgacggtta ccttcgccgg gccaaagcgc ggccacttcc
     3781 gcttccccgg tgcgtccgcc tgcggtgagt tggtcgtggc cgacatcgac atcgacccga
     3841 agctgccggc cgtagcgggc gtgcctctgg aactggccac agccgagggg gcgcgcgcgc
     3901 tgctgccggc ccgcccgccc gacggccaca agggcacttt cggcacggcc ctcatcgccg
     3961 ctggctcgat ccgctattgg ggcgcgccgg ccctggccgc tcgcggggcg tttcgcggcg
     4021 gcgcggggat ggtgacgctg gccgtgcccc aacggctgcg gccggcgctg gccgggcagt
     4081 tcccggaggc gacttatccg gccgtgaccg acaccgacac gctcggcgtg gccacggcca
     4141 acctgctgct gggcgtgctc gaccgcaccg gcgcgctgct ggtggggccg gggctgggcg
     4201 aggcggcgtc gttcctgagc gcgctgctcg atggcctgcg cagcccggcc gagggcgtgg
     4261 ccgtgccgcc gctgatcgtc gacgccgatg ggctgaacct gctggcgcag atgcccgact
     4321 ggcccggccg cctgccagcc aacactattc tgacgccgca tccgggcgag atggcccgcc
     4381 tggtgggccg gccgctgccg gcgctgcgcg acgaggaccg cgttgagttg gcccgcgacc
     4441 gcgccgcggc ctgggggcac gtcgtcctgc tgaagggggc ctacaccgtt atcgccgcgc
     4501 ccgatggccg ctcgacgctg ctgcccttcg ccaaccccgt gctggccgta gccggtagcg
     4561 gcgacgtcct gggcggggtc atcgtcggtc tgctggccca gggcgtggag ccatacgacg
     4621 cggccgtgtt gggcggctac ctccacggcg cggccgggca gctggcagcc ggctactggg
     4681 gcaacgccgg cctgctggcc agcgaattgg ccgactgggt gtcgcacgtg cgccgggcgc
     4741 tttccagccg gccagcatcg gcataacgct tgtttgactc tccaagccca atctaccttc
     4801 tttttgacaa gttatatcgc aagagatata atcttgtaag ataaattact gtgcaagcct
     4861 tcagatcgca gaaccaaacg cgctgtcatc tgttaggcct atcatacggg agcgtatcac
     4921 catgaccatc agacatatca ttgactaccc accgcggaca tctagcgtgc aaaatatctt
     4981 tcgcctgcta ctgggcgcag ccctgctgta tgccggtatc agccatctga ccttcgcccg
     5041 cacggaattc atggcgcagg tgccgtcttg gctgccgctg gatggcgatc tggtcgtcgt
     5101 tctgtcgggg attgtggaga tattgttggg cgttagcttg attgtcttgc ggcgctatcg
     5161 cgctctggtg gggtgggtgg cggcgatctt cttcgtcgtg atctttcctg gtaatatctc
     5221 gcagtacgtc aacggcattg atgcttttgg cctgaacacc gacacggccc gcttcgtgcg
     5281 cctgttgttc caaccgctgc ttgtcctgtg ggcgctgtgg agtactggcg cgtggcaggc
     5341 gtggcgctct cgccaccaga acgcgccaca gcgtcccgtg tcggaaacag gacgttaaca
     5401 gggtaatgtc caacgggccg gcgtgctcat ccgtctgccc tgtccaccac cgaggtcgca
     5461 tcagcatggc ctacgagcaa ctaaaactgg agaaccagat ctgcttcccc atctacgcgg
     5521 catcgcgact gattacgcgg gagtaccagc ccttgctcga tgagctgggg ctgacctatc
     5581 ctcagtacct ggccctgatg gtgttgtggg agcacaatgg cctgtcggtg aacgacatcg
     5641 gccagaagtt gctgctcaac accaatacgg tcacgcccct gctcaagcgg atggaagcac
     5701 aagggttggt gacacgccag cggtcggccg acgatgaacg gcgcgtgatt gtcagcctga
     5761 cgccacaggg gcaagcccta caggaacggg ccgcggccat tccgccccaa ttggccgccg
     5821 gtctgaatgc cgacggtgtg gacgaacagg ccctccaggc catgctggcc cagcttcaga
     5881 agatcatcca gcatctgtcc gcgaagcaga gcggtttgag cgagtagcat tatgctggcg
     5941 gcacgccggc cggtcacgtc ccggacacaa aacggccacg tcttttcagt tgtcaacaat
     6001 taacttgagg tctgaccggc tgtatgccgc gcaatggcat ggcgccagta gcacagcctg
     6061 ttaggctgtc gccgccagcc gcagacacag cctaacaggc tgtgctacag gctgtgctac
     6121 gctacgggct gtgcgttgag ccgcgcgaag accgacgact g
//
LOCUS       DAOSMQ010000145         6147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12875_length_6147_cov_5.009357, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000145 DAOSMQ010000000
VERSION     DAOSMQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12875_length_6147_cov_5.009357"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..610
                     /locus_tag="PK829_06295"
     CDS             419..610
                     /locus_tag="PK829_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40860.1"
                     /translation="MVTSPVIRKRFTADEYAPMAAAGVLTENDVMEAGRAPGDSPGRL
                     GRRANPWLSQFTMERITSA"
     gene            607..1161
                     /locus_tag="PK829_06300"
     CDS             607..1161
                     /locus_tag="PK829_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HOU40861.1"
                     /translation="MSTPIALPTLDSYWVLEPRLPAGQLVAGEYPSARNAAEADIKLG
                     RFVQFGITTFIDLTEAGEHGLRPYAGDIRALAAASGRSISHHRHPISDLGTPTPRTMR
                     AILDAIDEALAAGEMVYVHCYGGIGRTGTVIGCWLVRHGLSGEEALRRIAEWRAPTPD
                     GWRASPETPDQRALILSWREVEDE"
     gene            1158..1598
                     /locus_tag="PK829_06305"
     CDS             1158..1598
                     /locus_tag="PK829_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU40862.1"
                     /translation="MKPNAIRPIAICVCRHEGRILAAESEDPVKPQRFYRPLGGAIEF
                     GEYSAEAVVREFREELGVVLTDVRYLGTLENVFTYNGQHGHEIALVYDGRFADPSLYD
                     RPVIEGAEDNGAPFRAVWLSLAECAAPGAPPVYPTGLLELLGAK"
     gene            complement(1673..3409)
                     /locus_tag="PK829_06310"
     CDS             complement(1673..3409)
                     /locus_tag="PK829_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU40863.1"
                     /translation="MSLRYSDKPWVKSYDQGVPTNIEVPNYPVQHFLEEGARRIPGNT
                     AMVFQGGEISYRELNDIADAIAAGLAANGFKKGDRAVIFMPNLPQFLMIYYGILKAGG
                     IVIATNPLYTERELQHQLKDCGAETAFVLSRYYPLLKKVQKSGETKVKRVVVTYIKDY
                     LPGIKSVLYGLLKEKKAGDRVEVSPGDLTLKDFIALGKRSPKPNVTVTGEDIALLQYT
                     GGTTGLSKGAIGLHRNLVANALMVSKWINDWEYGRDSLLGAIPLFHSYGMVTAGIFPF
                     CAGGKVIIIPNPRDLHDVLENINKYKPAYFPGVPAMYVGINNHPDVAAGKYDLSSIRA
                     CISGSAPLLQETKRKFEELTGGKLVEGYGLTESHVATHANPINGLNPPGSIGLPLPGV
                     EARIVDPVEGEQELPVGAIGELIMRGPTIMAGYWNMPNETSVSLRDGWLYTGDIARMD
                     EQGYFYIEDRKKDLIICGGYNVYPREVEEVLARHPAVLEVTVAGVPDAKRGETVMAWV
                     VKRPGHENVTEKDIIEWSKGELAAYKYPRIIEFRNELPKTTVGKILKRELVREHKEQS
                     SNGSGQREKAAA"
     gene            3830..4663
                     /gene="pheA"
                     /locus_tag="PK829_06315"
     CDS             3830..4663
                     /gene="pheA"
                     /locus_tag="PK829_06315"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOU40864.1"
                     /translation="MNPSPPIVAFQGEHGAYSEQAVRRHFGPDVTTLPCRTFSAIIDA
                     LQAGRAAYGMLPVENSLAGMVVPAYDLLVDYDLHVQAEVILRVEHCLMAPPGTALANV
                     RRARSHPQALMQCETSLRRLGLEPVEYYDTAGAARDLAAQPEPGTAAIAAALAAETYG
                     LDILVHGLEDEDFNYTRFFVLGTSYPARRDPSKTSIILTTRHVPGALYAALGELARRG
                     INMTKIESRPRRNRPWHYLFYVDFEGHEEDEEIQAALLGILRHASFLKVLGSYPAGQP
                     E"
     gene            4686..5321
                     /locus_tag="PK829_06320"
     CDS             4686..5321
                     /locus_tag="PK829_06320"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HOU40865.1"
                     /translation="MPRASTAWVRVARAATAILILAAVVCLFPPIAGGGGSVVSFLSY
                     FTIQSNLIAATVLLWLAIRPPAAANVLRADIIRGAAVLYLAITGIVFALLLSGASAQM
                     PAALLWTNAVLHQLSPILLFLDWLVVPPAPGLTFRRALWWLVYPLAYLVYTLVRGAII
                     DWYPYPFLTPGLVGGYDGVLAYAAAITIGVLVFMGALFVSGRGMRRIVARG"
     gene            complement(5352..>6147)
                     /locus_tag="PK829_06325"
     CDS             complement(5352..>6147)
                     /locus_tag="PK829_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40866.1"
                     /translation="PRPPFPVSDTAAPGLTFLGYERGGERAETGEPLGLALWWAADAP
                     LPFLAVRLSLVDAAGAAHELLRGQPAYNTYPFHTWTTPAFVIDRQVARVPDDLPSGDY
                     ALRLELLDARGQPLYTVALGPLTVTQTERVFTPPPVANPVGASFGGEIALLGTNSSAG
                     GRTLELVWQAETQPAADYTVFVHVLRPDGTCCAWQADAMPRGGTYPTTRWRPGEVVTD
                     SYTITLPTDLPAGEYVVEVGLYRAETGERLTTATGEDAVRLPPIDR"
BASE COUNT          960 a         2069 c         1994 g         1124 t
ORIGIN      
        1 acggaccatt gtggcgagcg ctcgcgccgc tcgtccaccg cttttcagtt gtcagcaatt
       61 cttgagacct gaccggctgc atgctgtgca acggcgcgga accagtaaca cagcctgtta
      121 ggctgtcctc gccggccaca gcttgacagg ctgtgctacg ggctgtgcga ctgtagtaca
      181 gcctgttagg ctgtcatcgc cggccacagc ctaacagcct gacaggctgt gctacaggct
      241 gtgcgactgt agcacagcct gttaggctgt cgtcgccggc cacagcctaa cagcctgaca
      301 ggctgtgcta caggctgtgc tacaggctgt gctacgcgcg aagaccgact gttgaaaagc
      361 cctggccgct cgtccgggcg cgggcaaatg cgtctgagag tgaggtataa taacacctat
      421 ggttacctcg cccgtcatcc gtaagcgttt taccgccgac gagtacgcgc cgatggccgc
      481 ggccggcgtg ctgaccgaaa atgatgtgat ggaagccggc cgcgccccgg gcgattcccc
      541 aggccggctt ggccgtcgag caaatccttg gctgagccag ttcactatgg agaggataac
      601 cagcgcgtga gcactcccat cgccctccct accctcgatt cctattgggt gctggagccg
      661 cggctgccgg ccggccagct tgtcgccggc gagtacccca gcgccaggaa tgccgccgag
      721 gcagacatca agctgggtcg cttcgtccag ttcggcatca ctaccttcat cgacctgacc
      781 gaggcgggcg agcatggcct gcgcccttac gccggcgaca tccgcgccct ggccgcggcg
      841 agcggccgct ccatcagcca ccaccgccac cccatctccg acctgggcac gcccacgcca
      901 cggacgatgc gcgccatcct ggacgccatc gacgaggccc tggccgccgg cgagatggtc
      961 tacgtccact gctatggcgg catcggccgc acgggcacgg ttatcggctg ctggctggtg
     1021 cgccacggcc tgagcggcga ggaggccctg cgccggatcg ctgaatggcg tgcccccacg
     1081 cccgacggct ggcgcgcttc gccggagacg cccgaccagc gcgcgctgat cctcagttgg
     1141 cgcgaagttg aagacgaatg aaacccaacg ccatccgccc catcgccatc tgcgtctgcc
     1201 gccacgaggg gcgcatcctc gccgccgaat ccgaagaccc ggtcaagccg cagcgcttct
     1261 accggccgct gggcggggcc attgagttcg gcgagtatag cgccgaggct gtcgtccgcg
     1321 agttccggga ggagctgggc gtggtgctga cagacgtgcg ctatctgggc acgctggaga
     1381 acgtcttcac ctacaacggc cagcatgggc acgagattgc tctggtctat gacggccgct
     1441 tcgccgatcc gtcgctctat gaccggccgg tcatcgaggg cgctgaggac aacggcgcgc
     1501 cattccgcgc cgtctggctg tcgctggccg agtgcgccgc ccccggcgcg ccgccggtct
     1561 accccaccgg cctgctggag ctactgggcg caaaataaaa agggcgggtg gtgaaacccg
     1621 ccccctggcg ttactccgca atggcggtgg gttgcgcccg cccgggccgg aactacgccg
     1681 ccgccttctc gcgctggccg gagccgttgc tcgactgctc cttgtgctcg cgcaccagct
     1741 cgcgcttcag gattttgccg accgtcgtct tgggcagttc gttgcggaac tcgatgatgc
     1801 gcgggtactt ataggccgcc agctcaccct tgctccactc gatgatgtcc ttctcggtca
     1861 cgttctcgtg gccgggccgc ttgacaaccc aggccatgac cgtctcgccg cgcttggcgt
     1921 cgggcacgcc ggcgacggtc acttccagca ccgccgggtg gcgcgccagc acttcctcga
     1981 cctcgcgcgg atagacgtta tacccgccgc agatgatcag gtccttcttg cggtcttcga
     2041 tgtagaagta gccctgctca tccatccggg cgatgtcgcc ggtgtagagc cagccgtcgc
     2101 gcagggaaac ggacgtttcg ttgggcatat tccagtagcc ggccatgatc gtcgggccgc
     2161 gcatgatcaa ctcaccgatc gcgccgacgg gcagttcctg ctcaccctcg actggatcaa
     2221 cgatgcgcgc ctcgacgccc ggcagcggta ggccaatgga accgggcgga ttcaggccgt
     2281 tgatggggtt agcgtgggtc gccacgtgcg actcggtcag cccgtagccc tcgaccagct
     2341 tgccgccggt cagctcctcg aacttgcgct tggtctcttg caacagcggc gcggagccgg
     2401 agatgcaggc gcgaatggaa ctcaggtcat acttgcccgc ggccacgtcg gggtggttgt
     2461 tgatgccgac gtacatggct ggcacgccgg ggaagtaggc tggcttatac ttgttgatgt
     2521 tctccagcac gtcgtggagg tcgcgcgggt tggggatgat gatgaccttg ccgccggcgc
     2581 agaaggggaa gatgcccgcc gtgaccatgc cataggaatg aaagagcggg atggccccca
     2641 ggagcgaatc gcggccatac tcccagtcat tgatccactt cgacaccatc aacgcgttgg
     2701 ccaccagatt gcgatgcagg ccgatagcgc ccttagacag gccggtcgtg ccgccggtgt
     2761 attgcagcag agcgatgtcc tcgccggtga ccgtcacgtt tggcttgggg ctgcgcttgc
     2821 ccagggcgat gaagtccttg agcgtcaggt cgcctggcga cacctcaacg cggtcgccgg
     2881 ccttcttctc cttcagcagc ccatagagca cactcttgat gcccggcaga tagtccttga
     2941 tgtaggtgac gacgacgcgc ttgaccttcg tctcgccgct cttttgcacc tttttcagca
     3001 aggggtagta gcggctgagg acaaaggccg tctccgcgcc acagtccttg agctggtgtt
     3061 gcagttcgcg ctcggtgtag agtgggttcg tggcgatgac aatgccgccg gccttgagaa
     3121 tgccgtaata gatcatcaga aactggggca ggttgggcat aaagatgaca gcgcggtcgc
     3181 ccttcttaaa gccattggcc gccagccccg cggcgatggc gtcggcaatg tcattcagtt
     3241 cgcgatagct gatttcgccc ccctggaaga ccatcgccgt gttgccggga atgcgacgcg
     3301 cgccctcttc cagaaagtgc tgcaccgggt agttgggcac ttcgatgttg gtgggaacgc
     3361 cctggtcata gctcttcacc cagggtttgt cggagtaacg caggctcatg ggattgattc
     3421 tccttctctc aactgctgtc actgtcgccc ggcaagcaac ctggccctcg cgggcgggga
     3481 tggccggggc agcacacagg ggatgtaaag cgcgatggcg caccgcggcg ggtgtgaact
     3541 ccccaccggc agatgcggca acagatcgta ccgggatacg atccgcgatt ctgtcactat
     3601 agccgttttt ggtgtcatat catagttgcg tttgatacct tttcgacatg atcttcgtca
     3661 tgttttgcga aagaagatct cacgcaaagt cgcaaagaag caaaggcgca aaggagatat
     3721 aagttcttct ttgcgtcctt tgctctcttt gcgtcctttg cgtgagatct tctagcctcg
     3781 gcaatgcctg aggttgccag gcggcgcggc ggtcactaca cttcgcccca tgaacccatc
     3841 gccccccatt gtcgccttcc agggcgagca cggcgcttac tccgagcagg ccgtccgccg
     3901 ccacttcggg ccggacgtga cgacgctgcc ctgccgcacc ttctcggcca tcatcgacgc
     3961 gctgcaagcc ggccgcgccg cctacggcat gctgccggtg gagaactcgc tggccggcat
     4021 ggtcgtaccc gcctatgacc tgctggtgga ctatgacctg cacgtgcagg ccgaagtcat
     4081 tctgcgcgtg gagcactgcc tgatggcccc gccgggcacg gccctggcca atgtgcgccg
     4141 cgctcgctcc cacccgcagg cgctgatgca gtgcgagacg agcctgcggc ggctggggct
     4201 ggagccagtg gagtactacg acaccgccgg cgcggcccgc gacctggccg cgcagccgga
     4261 gccgggcacg gcggccatcg ccgccgccct ggccgccgag acttatggcc tggacatcct
     4321 cgtccacggc ctggaggacg aggacttcaa ctacacgcgc ttcttcgtcc tgggtacgtc
     4381 ttacccggcg cggcgcgatc ccagcaagac atcgatcatc ctgaccacgc gccatgtgcc
     4441 cggcgcgctc tacgcggccc tgggcgagct ggcgcggcgg ggcatcaaca tgaccaagat
     4501 cgagtcgcga ccgcgccgca accggccgtg gcactacctc ttctacgtcg atttcgaggg
     4561 ccacgaggaa gacgaggaga ttcaggcggc gctgctgggc atcctgcgcc acgcgtcgtt
     4621 cctgaaggtg ctgggatcat atccagcggg ccagccggag taactggaat aagaggagaa
     4681 caactatgcc ccgcgcatcc acagcctggg ttcgcgtcgc ccgcgcggcg acggcgatcc
     4741 tcatcctggc cgccgtggtc tgtctcttcc cgcccattgc cggcgggggc ggctcggtcg
     4801 tcagtttcct gagctacttc accatccaga gcaatctcat cgccgcgact gtgctgctgt
     4861 ggttggctat ccggccgccg gccgcggcga acgtcctgcg ggccgacatc attcgcggcg
     4921 cggccgtgct ctacctggcc atcaccggca tcgtctttgc cctgctgctg tccggcgcgt
     4981 cggcgcagat gccggcggcg ctgctgtgga cgaacgccgt cctgcaccaa ctctcgccca
     5041 tcctgctgtt cctcgactgg ctcgtcgtcc cgccggcgcc tgggctgacc ttccggcggg
     5101 cgctgtggtg gctggtctac cccctcgcct acctcgtcta caccctcgtc cgtggggcga
     5161 tcatcgactg gtatccgtac ccgttcctga cgccgggcct tgtcggcggc tacgacggcg
     5221 tactggccta tgccgcggcc atcacgattg gggtgctggt gttcatgggg gcgctgtttg
     5281 tcagtgggcg ggggatgcgg cggattgtgg cgcgtgggta acgccggtag gggcaggtct
     5341 cagacccgcc cctaccggtc gatcggcggc aggcgcaccg cgtcctcccc cgtcgccgtc
     5401 gtcaaccgct cccctgtctc cgcccggtac agccccactt cgaccacgta ctcccccgcg
     5461 ggcaggtcgg tgggcagggt gatggtgtag ctgtcggtaa cgacttcgcc cggccgccag
     5521 cgcgtcgtcg ggtacgttcc gccgcgcggc atggcgtcgg cctgccaggc gcagcacgtc
     5581 ccatctggcc gcaggacgtg gacgaagacg gtgtagtcgg ccgccggctg cgtctccgcc
     5641 tgccagacca gttccagcgt ccggccgccg gcgctgctgt tcgtgcccaa cagggcgatc
     5701 tcgccgccga acgacgcgcc gacggggttg gccactggcg gcggggtgaa gacgcgctcc
     5761 gtctgtgtca ccgtcagcgg gccgagggcg accgtgtaga gcggctgccc ccgcgcgtcg
     5821 agaagctcca ggcgcagcgc gtagtcgcca gatggcaagt cgtcgggcac gcgggccacc
     5881 tgccggtcga tgacaaaggc cggcgtcgtc caggtgtgga aggggtaggt gttgtaggcc
     5941 ggctggccgc gtaatagctc gtgggccgcg ccggccgcgt ccaccagcga cagccgcacg
     6001 gccaggaagg gcaggggcgc gtcggccgcc caccacagcg ccagccccag cggctcgccg
     6061 gtctccgccc gctcgccgcc gcgctcatag cccaggaagg tcagccccgg cgcggctgtg
     6121 tcgctcactg ggaagggcgg gcgcggt
//
LOCUS       DAOSMQ010000146         6117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12976_length_6117_cov_5.041241, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000146 DAOSMQ010000000
VERSION     DAOSMQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12976_length_6117_cov_5.041241"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..838)
                     /locus_tag="PK829_06330"
     CDS             complement(101..838)
                     /locus_tag="PK829_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40867.1"
                     /translation="MTDPTPDTPPPNRPTGTLAKERLAVRARWNQMVNTPEAISDRRI
                     RKWSELWETVILSLATLITAWAGYQAGQWNSIQTAMNTHAHTLQLQAGVLDDRAGKQQ
                     LVDVVAFTQWVNATVDGNQAQAAIYEERFRPEFLPAFNAWQATTPLVNADAPATPFAM
                     PEYHLGLIDDAAQLRREAETLNISAVDAGQTADAYTLAIVILAGALLLAGLANRFEWE
                     ELRIVVVIAALFILLYSVIRIIVLPMA"
     gene            1079..1621
                     /locus_tag="PK829_06335"
     CDS             1079..1621
                     /locus_tag="PK829_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40868.1"
                     /translation="MSQPLTLRIRPGESVRFPNHCVACGQPATERLTLHNRRGQLTRR
                     LDAPLCADCARQLARRSGREEQLRRTGALVAAVVGVLALVAVVALAGGAWWLRGLLGA
                     IAASAAAAFVWALFRRRAEAAQLPETRAVRAAAQIVDFTWRDMTLVFADEAMATAVRE
                     LNAALLPAAVPQLEAGEPTI"
     gene            1651..2436
                     /locus_tag="PK829_06340"
     CDS             1651..2436
                     /locus_tag="PK829_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOU40869.1"
                     /translation="MKVVVCVKQTPSTTATISVKDGAVSWEDPGGKPNVVNPWDEYAI
                     EEAIRFKENRGASDAVALCMGDSSSEEALKTCLAMGCTEAVLVSDPALLGSDTLGTAR
                     TLAAAINKIGDVQVALFGRQAIDGDSGHTPVQVARVLGWTPLTYVSAIESVDGGSITV
                     HRLTDDGKETVTAKLPVVISVVKEINEPRYPSFMGIRKASKAVIPNWSAGDIGASAAA
                     ARANWSNVYAVPPREGEIEMIAGDTVEEQARNLVNKLFEEKVI"
     gene            2433..3407
                     /locus_tag="PK829_06345"
     CDS             2433..3407
                     /locus_tag="PK829_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOU40870.1"
                     /translation="MSGVWVYVENRDGAISAISREALGAARQVADGLGQPLTALVFGQ
                     GVGPVCAAAFDLGADAVVGADDATLADGRLEAHGPLVVKLAQERQPAVILIGASTRGR
                     DLAAWTAADLDTGAVADAIDIKVEGGVVKATRPVYTGKLLCEAYVTGTPQVVTLRGRA
                     FAQAASTGKTGTPEMVAAAVAEDAIPTKITGFEGKGGKISLTDAGKIVSGGRGVGGPE
                     GFEPLRELADVLGAALGASRATVDAGWIPYEHQVGQTGKTVAPDLYIAAGISGAIQHQ
                     AGMRSSKVIVAINKDPDAPIFKLARYGIVGDLFKIVPALTAEFKKRLN"
     gene            3479..4417
                     /gene="rsgA"
                     /locus_tag="PK829_06350"
     CDS             3479..4417
                     /gene="rsgA"
                     /locus_tag="PK829_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOU40871.1"
                     /translation="MNGLVIKAQSGFFTVQPDDGGEPVISRVRGRLKQERLRTDLVAV
                     GDRVTVQVNADGSGSVQSVAERHSALSRTRVAAQDRSLRTDTEQVLVANPDQVVFVFS
                     VASPAFSPRKLDRFLVVAEMNRLPAVICANKVDLVAPEEARDMFRVYEAIGYPVLYTS
                     VVTGEGVEALREALRGKLSVLAGSSGVGKSSLLNAVQEGLGLRVKEVSEATGKGMHTT
                     RHTELIPLEIGGYVADTPGIRGLALFDLEPTEVDAYFREIGPLVAQCQFSDCTHTHEP
                     HCAVLAAVADGRVSAERYESYLRLREEQEELEDKRY"
     gene            complement(4414..5412)
                     /locus_tag="PK829_06355"
     CDS             complement(4414..5412)
                     /locus_tag="PK829_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018073054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU40872.1"
                     /translation="MDTFDSTPSGAPIPLTILTGFLGAGKTTLLNRLLRGGHGLRVAV
                     LVNDFGAINIDAELVVDVADDVISLANGCICCSLRDDLVEAVLRAMARPEQPEYILLE
                     ASGVADPAGITLAFHTPRLRDRIRLDSVTCVLDAQQVFAYPNYPELLQLQLHQIAFAD
                     MVVLNKVDLATPEQLRQIHALIDQHFNNIRIVETSYCDVPYEILLGVGRFDPAVLIAA
                     APNGSHDHHHGHDARPAFDTWSFETDRALSLAALQEAARKLPGGVFRCKGIVRAAEEP
                     EQPAVLQVVCRRVDITRLASWEGRRPATRIVTISAPGSVRGDDLRRWFEGCIAPLR"
     gene            complement(5498..>6117)
                     /locus_tag="PK829_06360"
     CDS             complement(5498..>6117)
                     /locus_tag="PK829_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOU40873.1"
                     /translation="RVEAEADINRDGKPDKLTAKYAQGVDVFVTEMAVDVVRLWALKQ
                     GVSPFIGAFTLDNFHTTHYAFGYLANLVQPRLAMASHISFDRELIGEMMAGVRMHYPG
                     QFAFGIDHTVVNVTRDRLWIREAALPETTNTARATSEWLLQNEFDGTVPPPVIAQNPF
                     SPIQEQYIRDQEIDPALFTPADQMRQWAHPLPPTFPLDPAKTLGL"
BASE COUNT         1038 a         2008 c         2080 g          991 t
ORIGIN      
        1 cggccttccc atccaacatc gacgcgattg agatggcccg gtaagcggcc agtggcggtt
       61 acaaagcaaa ccgctgcggc cgggccgctg cggccgggcg tcaggccatt ggcagcacga
      121 tgatgcgaat gacgctgtag agcaagatga acagcgccgc gatgacaacg acgatacgca
      181 gttcttccca ctcaaaccga ttggccaggc cagccagcag cagcgccccg gccaggataa
      241 cgatggccag cgtgtaggcg tcggccgttt gcccggcatc gacggcgctg atgttgagcg
      301 tctccgcctc ccgccgcagc tgggccgcgt catcgatcag gcccaagtga tattctggca
      361 tcgcgaacgg cgtggccggc gcgtcggcgt tgaccagcgg ggtggtcgcc tgccaggcgt
      421 tgaaggccgg cagaaactcc gggcgaaaac gctcctcata aatggcggcc tgcgcctggt
      481 tgccgtcgac cgtggcgttg acccactggg tgaaggcgac cacatcgacg agttgttgct
      541 tgccggcgcg gtcgtccagg acgccggcct gcaactgcaa ggtgtgggcg tgcgtgttca
      601 tggctgtctg gatgctgttc cactgcccgg cctgatagcc ggcccaggcg gtaatgagcg
      661 tggccaacga caggataacc gtctcccata gctccgacca tttgcggatg cgacggtcgc
      721 taatcgcctc cggcgtgttg accatctggt tccagcgcgc gcggacggcc aggcgttcct
      781 tggccagcgt gccggtcggc ctgttgggcg ggggggtgtc gggtgttggg tcagtcatgg
      841 tgtcaatccg tggccctaat cgttcgcgat ggagcggaca gccgctggga ttttccccga
      901 gacgacgcgg ggcggcaact aatccgcttg gccctgcttg gcccggccgc taacctgatg
      961 cgcattgctc ctaacgcgcc gcggcactat acttgaacct gttagggcta ggaacatgcc
     1021 atgtgtcacc ccaaaacctg acacatagca catgacagcc gcccggcaat ccgttatcat
     1081 gagtcaaccg ttgacgctcc gcattcgccc cggcgagtcg gtgcgctttc ccaaccattg
     1141 tgtcgcctgt gggcagccgg cgaccgagcg cctgacgctt cacaaccgac gtggccagtt
     1201 gacgcgccgt ctggacgcgc cgctgtgcgc cgattgcgcc cgccagctgg cccgccgctc
     1261 cggccgcgag gaacagctac ggcgtaccgg ggcgctggtt gccgccgtgg tcggggttct
     1321 ggccctggtc gccgtcgtgg cgctggccgg cggggcctgg tggctgcgcg ggctgctggg
     1381 cgcgatcgcc gcgagcgcgg ccgcggcttt cgtctgggcg ctcttccgcc ggcgggccga
     1441 ggcggcgcaa ctgccggaga cgcgcgcggt gcgcgcggcg gcgcagatcg tggactttac
     1501 ctggcgcgat atgaccctgg tcttcgccga tgaggcgatg gccacggccg tgcgcgaatt
     1561 aaatgcggcc ctgctgcccg cagccgtacc gcagctcgaa gcgggcgaac caacaatctg
     1621 aacgtcctag tcaactttgg aggttgaagt atgaaagtag tcgtgtgcgt gaaacagacg
     1681 cccagcacaa ccgccaccat ctctgtcaag gatggcgcgg ttagctggga ggaccccggc
     1741 ggcaagccca acgtggttaa cccgtgggac gagtacgcca ttgaagaagc cattcgcttc
     1801 aaggaaaacc gtggcgcgtc cgacgccgtc gccctgtgca tgggcgactc gtcgagtgaa
     1861 gaggcgctga agacctgcct ggcgatgggc tgcaccgaag cggtgcttgt ctccgacccg
     1921 gcgctgctgg gcagcgacac actgggcacg gcccgcacgc tggccgcggc catcaacaag
     1981 atcggcgatg tgcaggtggc gctgttcggc cggcaggcca tcgacggcga ctccggccac
     2041 acgccggtgc aggtcgcccg tgtactgggc tggacgccgc tgacctacgt ctcggccatc
     2101 gaatcggttg acggcggcag catcaccgtc catcgcctga ccgacgacgg caaggagacg
     2161 gtcacggcca agctgcccgt ggtcatcagc gtcgtcaagg agatcaacga gccgcgttac
     2221 ccatcgttca tgggcattcg caaggccagc aaggcggtca tccccaactg gtcggcgggc
     2281 gacattggtg cgagcgcagc cgcggcccgg gccaactggt cgaacgtcta cgcagtcccg
     2341 ccacgcgagg gcgagatcga aatgatcgcc ggcgacaccg tcgaagagca ggcgcgcaac
     2401 ctggtcaata agctgtttga ggagaaggtc atatgagcgg cgtatgggtc tatgtcgaga
     2461 atcgcgacgg cgcgatcagc gccatcagcc gcgaggcatt gggcgcggcg cggcaggttg
     2521 ccgacggcct cggccagcct ctgacggcgc tcgtgtttgg gcagggcgtt ggccccgtct
     2581 gcgcggcggc ctttgacctg ggcgctgacg cggtcgtcgg cgccgacgat gccacgctgg
     2641 ccgacggccg cctggaagcc cacggcccgc tggtcgtcaa gctggcccag gagcggcagc
     2701 cggcggtcat cctcataggt gcgtccaccc gtggccgcga cctggccgcc tggacggcgg
     2761 ccgacctgga caccggcgcg gtggccgacg ccatcgacat caaggtcgag ggcggcgtgg
     2821 tcaaggcgac gcggccggtc tacaccggca agctgctgtg cgaggcctat gtcaccggca
     2881 cgccgcaggt cgtcaccctg cgcggccgcg ctttcgccca ggccgcctcc acgggcaaga
     2941 ccggcacgcc ggagatggtc gccgcggccg tggccgagga cgccatcccg accaagatca
     3001 ccggcttcga gggcaagggc ggcaagatta gcctgaccga tgctggcaag atcgtcagcg
     3061 gcggccgcgg cgtcggcggg ccggaaggct tcgagccgct gcgcgagctg gctgacgttc
     3121 tcggcgcggc cctgggcgct tcccgcgcca cggtcgacgc cggctggatt ccctacgagc
     3181 accaggttgg ccagaccggc aagacggtcg cccccgacct ctacatcgcc gccggcatca
     3241 gcggcgcgat ccagcaccag gccggtatgc gcagctcgaa ggtcatcgtg gccatcaaca
     3301 aagaccccga tgcgcccatc ttcaagctgg ctcgctacgg catcgtcggc gacctgttca
     3361 agatcgtccc ggcgctgacg gccgagttca agaagcgact caattagaaa ggggccaggg
     3421 gccaggggcc agggaagaat gctcttctct ggtccctgga acctggaacc tggaacctat
     3481 gaacggtctt gtcatcaaag cccagagcgg cttcttcacc gttcagcctg acgacggcgg
     3541 cgagccggtc atcagtcgcg tccgcgggcg gctgaagcag gagcgcctcc gcaccgacct
     3601 ggtggccgtt ggcgaccgcg tcaccgtcca ggtcaacgcc gacggctccg gctcggtgca
     3661 gtcggtggca gaacgccact cggccctctc gcgtacgcgc gtggcggcgc aagatcgttc
     3721 gttaaggacc gacaccgagc aggtgcttgt cgccaacccc gaccaggtcg ttttcgtctt
     3781 ctccgttgcc agccctgcct tcagcccgcg caaactcgac cgcttcctcg tcgtggccga
     3841 gatgaaccgt ctgccggcgg tcatctgcgc caacaaggtt gacctggtgg cgccggaaga
     3901 agcgcgcgac atgttccgcg tctacgaggc catcggctac cccgtgctgt acaccagcgt
     3961 cgtcaccggc gagggcgtcg aggcgctgcg cgaggcgctg cggggcaagc tgtcggtgct
     4021 ggccgggtcg tcgggcgtgg gcaagtcgag cctgctcaac gcggtgcaag aggggctggg
     4081 gctgcgcgtg aaggaagtga gcgaggccac gggcaagggg atgcacacga cgcgccacac
     4141 cgagttaatc cctctggaga tcggcggcta cgtggccgac acgccgggca tccgcggcct
     4201 ggccctgttc gacctggagc caaccgaagt ggacgcctac ttccgcgaga tcggcccgct
     4261 ggtggcgcag tgccagttta gcgactgcac ccacacccac gagccgcact gcgccgtcct
     4321 agccgccgtg gccgacggcc gcgtgtcggc cgagcggtat gagagctatt tgcggttgcg
     4381 ggaggagcag gaggagttgg aggataagcg gtattagcgc aacggcgcta tgcacccttc
     4441 aaaccaccgc cgcaaatcat ccccacgcac actccccggc gcgctaatcg tcacgatccg
     4501 cgtcgccggc cgtcgtccct cccaactcgc cagccgggtg atgtccacgc gccggcagac
     4561 gacctgcaac accgccggct gctccggctc ctcggccgcg cgcacgatgc ccttgcagcg
     4621 gaagacgccg cccggcagct tccgcgccgc ctcttgcagc gccgctagag aaagcgcccg
     4681 gtcggtctcg aagctccacg tgtcgaacgc cgggcgggcg tcgtggccgt ggtgatggtc
     4741 gtggctgccg tttggcgcgg cggcgatgag cacggccggg tcgaagcggc cgacgcccag
     4801 caagatctcg tagggcacgt cgcaatagct ggtttcgacg atgcgaatgt tgttgaagtg
     4861 ctggtcgatc agggcgtgga tctgccgcag ctgttccggc gtggccagat cgaccttatt
     4921 gaggacaacc atatcggcga aggcgatctg atgcagttga agctgcaaca gttccgggta
     4981 gttgggatag gcgaagacct gctgcgcgtc gagcacacag gtgacgctat cgagccggat
     5041 gcggtcgcgc aggcgcggcg tgtggaaggc cagcgtgatg ccggccgggt cggccacgcc
     5101 gctggcctcc aggaggatgt actccggctg ttccggccgc gccatcgccc gcagcaccgc
     5161 ctcaaccagg tcgtcgcgca ggctacagca gatgcagcca ttggccaggc tgatgacatc
     5221 gtcggccacg tccaccacca gctcggcgtc gatgttgatc gccccgaagt cgttgacgag
     5281 cacagccacc cgcaggccat ggccgccgcg caacaggcga ttgagcagtg tcgtcttgcc
     5341 cgcacccaga aagccggtca ggatcgtcaa cgggatcggc gcgccggacg gggtggaatc
     5401 gaacgtatcc atagcggcgg gactctccac gttgccaccg gcccccaagg ttaggcactg
     5461 ggggccggcg acctaagacc acaacgacat cgactgccta taggcccagc gtcttggccg
     5521 ggtccagcgg gaatgtcggc ggcagcggat gcgcccactg acgcatctga tcggccggcg
     5581 tgaacagtgc cgggtcgatc tcctggtcac ggatatactg ctcctgaatg ggcgagaacg
     5641 ggttctgggc aatcaccggc ggcggcacag tgccgtcgaa ctcgttctgc agcagccact
     5701 ccgacgtcgc ccgggccgtg ttggtcgttt cgggcagggc cgcctcgcga atccacaggc
     5761 gatctctggt gacgttgacg acggtgtggt cgatgccaaa ggcgaactgg ccgggatagt
     5821 gcatgcgcac gccggccatc atttcgccga ttagctcgcg atcgaacgat atgtggctgg
     5881 ccatggccag ccgcggctgc accagattgg ccaggtagcc gaaagcgtag tgggtcgtgt
     5941 ggaagttgtc gagggtgaaa gcgccgatga acggcgagac gccctgcttc agcgcccaca
     6001 agcgcacgac atcgacggcc atctcggtca cgaacacatc cacgccctga gcatacttgg
     6061 ccgtcagctt gtcgggcttg ccgtcacgat tgatgtccgc ttccgcttcg acacgaa
//
LOCUS       DAOSMQ010000147         6111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_12994_length_6111_cov_4.155548, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000147 DAOSMQ010000000
VERSION     DAOSMQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12994_length_6111_cov_4.155548"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PK829_06365"
     CDS             complement(<1..1180)
                     /locus_tag="PK829_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOU40874.1"
                     /translation="MQNHGAFVSHYGVENHGIKNAETVYWNLTTSQLYEQAIRRHEAA
                     IAHLGPLVVQTGSHTGRSPNDKFVVQEPSSEPDIWWGKVNRPFSQENFDYLYRKMLAY
                     TQNRDIFVFDGYVGADPKYRMPVRIITEYAWHNLFARNMFIREFDPQKLANHVPQFTV
                     IDMPRLKAEPELDGTKSQTFILLDFGRRLVLIGGSEYSGEIKKSIFTAMNYFMPKQGV
                     MSMHCSANFGKDRDDVALFFGLSGTGKTTLSSDPTRTLIGDDEHGWSDDGVFNYEGGC
                     YAKVIKLDPKGEPEIYETTRRFGTILENVIMDTGTRRVDLDDGSLTENTRASYPISHV
                     SNAVRDGVAGHPTHVFFLTADAFGVLPPIARLTNAQAMYHFLSGYTAKVAGTERGVTE
                     P"
     gene            1339..1737
                     /locus_tag="PK829_06370"
     CDS             1339..1737
                     /locus_tag="PK829_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOU40875.1"
                     /translation="MNQDNAIRKILTEARTIAVVGFSSNPAKAGYYVPAYLQQHGYRV
                     IPVNPNLSEGLGETAYPALADVLESIDLVLIFQRSENVPPFVDQAIAVGAKAVWLQLG
                     IAHDAAAAKARAAGLDVVQDACMLVEHRHR"
     gene            1820..2230
                     /locus_tag="PK829_06375"
     CDS             1820..2230
                     /locus_tag="PK829_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40876.1"
                     /translation="MSDDIKTLKDAEKANDGLGDENSRDTEGHDGGMAWLPGLGLILV
                     GVIFLLSNFTGFQLHNWWALFILIGAFGAFGSAISAWRSTGRFGREARGSLIGGLAIL
                     FTAAIFLFGWNWATVWPGYLILGGLAALLTAGGD"
     gene            complement(2227..2634)
                     /locus_tag="PK829_06380"
     CDS             complement(2227..2634)
                     /locus_tag="PK829_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40877.1"
                     /translation="MPRLSQWYIKLSFGYLLAGFTVGALLLANKGRPLHPALWGLLPA
                     HIEWLLMGWVAQLTLGVAFWILPRFWQAPRRGRTTGAVVAIVLLNAGILLVSLGAVLG
                     LGAWATAAGRLLEIGAAAAFAHAAWPRIVGRAG"
     gene            complement(2636..3067)
                     /locus_tag="PK829_06385"
     CDS             complement(2636..3067)
                     /locus_tag="PK829_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40878.1"
                     /translation="MPPLTRWHIKTAFISLAAAMTLGVALALALVLPLPGWVVYLSPA
                     FFHLIMVGWVTQMIFGVIFWMFPIVSRARPRGNERLGWATYGLLNGGLLLRVVAEPAN
                     SVSPGGGWGWVLVASALMQWLAAVFFVVNAWPRVKEKYRGE"
     gene            complement(3072..4133)
                     /locus_tag="PK829_06390"
     CDS             complement(3072..4133)
                     /locus_tag="PK829_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40879.1"
                     /translation="MTRLPLLFLAILALLAGIWAGWLRIGWAWPNPQALLPAAHGPLM
                     VSAFLGTVISLERAVALRRRAAYLPPLLAGLGGLALIIGLSWVLAAWLLLAAAVGLAV
                     VFAVIVRQHPAVYTVTMAVGAGAWLVGNGLLAAGRPVYAAVPWWVAFLVLTIAGERLE
                     LSRVLRLSPRVINLFILSAAVFLVGVALTLVAYTEGTQLAGLGLLLLAVWLLRYDLAR
                     HNIRRRGLTRFIAVCLFGGYIWLAAGGALWLVAGGAVAGPLYDAMLHVLFLGFVFAMI
                     FGHAPLIFPSVLGRPMAYLPAVYAPLVVLHGSLALRVAGDLLGQMAARRWGGLLNGVA
                     LLLFLATTIAALAIGARKE"
     gene            complement(4268..4588)
                     /locus_tag="PK829_06395"
     CDS             complement(4268..4588)
                     /locus_tag="PK829_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU40880.1"
                     /translation="MDKPYFYVDNVAALAPDVPADAILSRTFYKDDRLSAMVFSFAPG
                     QELSEHTAAVPAIIHILSGRARLTLGEDETTAEAGTWVRMEPRLPHVVVAETAVTMLL
                     LMLR"
     gene            complement(4686..4913)
                     /locus_tag="PK829_06400"
     CDS             complement(4686..4913)
                     /locus_tag="PK829_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40881.1"
                     /translation="MTTDAPMTTERLRELPVEEVMARWPATAEVFNGLALACVGCALA
                     PFCTVQDAATTYDLPPQALSDELLAVIAAGR"
     gene            complement(4977..5414)
                     /locus_tag="PK829_06405"
     CDS             complement(4977..5414)
                     /locus_tag="PK829_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOU40882.1"
                     /translation="MTRKYTLLALLAALALVLALAACGGGGGEETAAPANDNAVAEAP
                     AGDDGSLHGDPEAGKVQFDMVCIACHGPGGEGIEGLGKPFTTSEFLLTVDDHELLNFI
                     KTGRPISDPANTTGVDMPPKGGNPALTDEQLTDIIAYIRTLHE"
     gene            complement(5442..5921)
                     /locus_tag="PK829_06410"
     CDS             complement(5442..5921)
                     /locus_tag="PK829_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOU40883.1"
                     /translation="MLALALLLALAACGGGADTTQPPEILYGQDVCDECDMIISEEKY
                     AAAYWTAAGEARRFDDVGEMLVFLARNPEATASVWVHDVNSAAWLPAEEAWFVMNSGL
                     RTPMGTGIAVLADEQSAQALAFDQTAAEVMRFSEILARAEAGELENVAGMGDNEMGD"
BASE COUNT         1074 a         2077 c         2001 g          959 t
ORIGIN      
        1 gcggctcggt cacgccgcgc tcggtgccgg cgaccttggc cgtatagccg ctcaggaagt
       61 gatacatcgc ctgggcgttg gtcagcctgg cgatgggcgg cagcacgcca aaggcgtcgg
      121 ccgtcaggaa gaagacgtgg gtcgggtggc cggccacgcc gtcgcgcacg gcattgctga
      181 cgtgggaaat ggggtagctg gcgcgggtgt tctcggtcag cgagccgtcg tccaggtcca
      241 cacggcgcgt gccggtgtcc atgatgacgt tctccaggat ggtgccaaag cggcgggtgg
      301 tctcatagat ctccggctcg cccttggggt caagcttgat gaccttggca tagcagccgc
      361 cctcgtagtt gaaaacgccg tcatcgctcc agccgtgctc gtcgtcgccg atcagcgtcc
      421 gcgtcgggtc gctgctcagc gtggtcttgc ccgtgccgct caggccgaag aagagggcca
      481 cgtcgtcgcg gtccttgccg aagttggcgc tgcaatgcat cgacatgacg ccttgcttgg
      541 gcatgaagta gttcatggcc gtgaagatgc tcttcttgat ctcgccgctg tactccgaac
      601 caccaatgag caccagacgc cggccgaagt cgagcaaaat gaaggtctgg ctttttgtgc
      661 cgtccagttc cggctcagcc ttcagccggg gcatgtcgat aactgtgaac tggggcacat
      721 ggttggccag cttctgcgga tcgaactcgc ggatgaacat gttgcgggcg aagaggttgt
      781 gccaggcgta ctcggtgatg atgcgcacgg gcatgcggta cttcgggtct gcgcccacgt
      841 agccgtcgaa gacgaagatg tcgcggttct gggtgtaggc cagcatcttg cggtagaggt
      901 agtcgaagtt ctcctgcgaa aaggggcggt tgaccttgcc ccaccagatg tccggctcgc
      961 tcgacggctc ctggacgacg aacttatcgt tgggggagcg gccggtgtgg ctaccggtct
     1021 gcacgaccag cgggccgagg tgggcgatgg ccgcctcgtg ccggcggata gcctgctcat
     1081 agagttggga agtagtcaga ttccagtaca ccgtctcggc gtttttgatg ccatgatttt
     1141 caacgccata gtgactgacg aatgcgccat ggttttgcat ggggtttact cctgtggttg
     1201 gttgatggaa atgttggtcg cgttcgcgtg gctcctcaag tagtgtatgc caatcagggg
     1261 cggttggcaa ttgacacacg gcgcgctttg tcatctattc cttgcgcaga atgtcaccgt
     1321 ttggatacag ttagcggcat gaatcaagac aacgccatcc gcaaaatact gaccgaagct
     1381 cgcaccattg ccgtcgttgg cttctcttcc aaccccgcta aggccggcta ttacgtgccc
     1441 gcctatttgc aacagcacgg ctaccgcgtc atcccggtga accccaacct gagcgagggg
     1501 ctgggcgaga ccgcctaccc cgcgctggcc gacgtgctgg agtcgattga cctggtgctg
     1561 atcttccagc gcagcgagaa cgtgccgccg tttgtcgatc aggccatcgc cgtgggggcc
     1621 aaagcggtct ggctgcaact gggcatcgcc cacgacgccg cggcggcgaa ggcccgggcc
     1681 gctggcctcg acgtggtgca agacgcctgc atgcttgtcg agcaccggca ccgctgagcc
     1741 ttcgcgccgc gcaactgccc gcgccgcgcc gcgcaaccgc ccgcgccgcg ccgcgtatag
     1801 atgagcggag gttgagacaa tgagcgacga tatcaagact ttgaaggacg cggaaaaggc
     1861 aaacgatggg ctgggcgacg agaacagccg ggacacagag ggccacgacg gcggcatggc
     1921 ctggctgccc gggctggggc tgatcctcgt cggcgtcatc ttcctgctga gtaacttcac
     1981 tggcttccag ttgcacaact ggtgggcgct gttcatcctc atcggcgcgt ttggggcgtt
     2041 cggcagcgcc atcagcgcct ggcggtcgac ggggcgcttt gggcgcgagg cccgcggctc
     2101 actcattggc ggcctggcca ttctgttcac ggcggccatc ttcctgttcg gctggaactg
     2161 ggccacggtc tggcccggct acctgatcct cggcggattg gccgcgctgc taacggccgg
     2221 gggcgactag cccgcccggc cgacaattcg cggccacgcc gcatgggcaa aggccgccgc
     2281 cgcgccgatc tccagcagac ggccggcggc agtggcccac gcgcccagcc ccagtaccgc
     2341 ccccagcgac accagcagga tgccggcgtt gagcaggacg atggccacga ccgcgcccgt
     2401 cgtccggccg cggcgcggcg cttgccagaa gcgcggcaga atccagaagg ccacgcccag
     2461 cgttagctgc gccacccagc ccatcagcag ccactcgata tgggccggca gcaggcccca
     2521 cagcgccggg tgcaacggcc ggcccttgtt ggccagcagc agcgcgccca cggtgaagcc
     2581 cgccagcaga tagccgaacg acagcttgat gtaccactgg ctcagacgcg gcatctcact
     2641 cgccccggta cttctctttg acgcgcggcc aggcgttgac gacgaagaat accgccgcca
     2701 gccactgcat cagcgccgag gccaccaaca cccagcccca gcccccgccc ggcgacacgc
     2761 tgttggccgg ctcggccacg acgcgcagca gcagaccgcc gttcagcagc ccataggttg
     2821 cccagcccag ccgctcgttg ccccgcggcc gcgcccggct gacgatgggg aacatccaga
     2881 agatgacgcc aaagatcatc tgcgtcaccc agccgaccat gatcaggtgg aagaaggccg
     2941 gcgacaggta gaccacccag cccggcagcg gcagcaccag ggccagcgcc agggccacgc
     3001 ccagcgtcat cgccgcggcc agggagatga aagcagtctt gatgtgccag cgagtcagtg
     3061 gcggcacggg tctactcctt gcgcgcgccg atggccagcg cggcgatcgt cgtcgccagg
     3121 aacagcagca gggccacgcc gttgagcagc ccgccccagc gccgcgcggc catctgcccc
     3181 agcaggtcgc cggccacgcg cagcgccaat gagccgtgga ggacgacgag cggcgcgtag
     3241 acggcgggca gataggccat cggccgcccc agcaccgacg ggaagatgag cggcgcgtgg
     3301 ccgaagatca tggcgaagac gaaacccagg aaaaggacgt gaagcatggc gtcgtagagc
     3361 ggcccggcca cggccccacc ggcgacgagc cacagcgccc cgccggcggc caaccagatg
     3421 tagccgccga acagacagac ggcgataaag cgcgtcaggc cgcgccggcg gatgttgtgt
     3481 cgggccaggt cgtagcgcag cagccagacc gccagcaaca acagccccag cccggccaat
     3541 tgcgtgccct cagtgtaggc cacgagcgtc agcgccaccc cgaccagaaa gacggccgcg
     3601 ctgaggatga acaggttgat cacccgcggc gatagccgca ggacccggct caactccagc
     3661 cgctccccgg cgatggtcag caccagaaag gccacccacc agggcacggc ggcatagacc
     3721 ggtcgcccgg cggccagcag cccgttgccc accagccagg ccccggcgcc cacagccatg
     3781 gtcaccgtgt ataccgccgg gtgttgccgg acgatgacgg cgaacacgac cgccagcccg
     3841 acggcggcgg ccagcagcag ccacgcggcc agcacccagg atagtccgat gatgagcgcc
     3901 agcccgccca gcccggccag cagcggcggc aaataagccg cccgccgccg cagagccacg
     3961 gcccgctcca ggctgatgac cgtacccagg aaggcggaga ccatcagcgg cccgtgggcg
     4021 gccggcaata gcgcctgtgg gttgggccag gcccagccga tgcgcagcca gccggcccag
     4081 atgccggcca gcagggccag gatggccaga aacagtaatg gcagacgagt cattaataga
     4141 cagggcaagc tcacgccaag ccgccaagaa agcaaagtcg ccaaggaaga agctctctct
     4201 tctttatctt cttagcgcct ttgccccttc gcgcctttgc gtgagctttg cgtgagcttc
     4261 tatcactcta tctaagcatc agcagaagca tcgtcacggc cgtctcggcc acgacgacat
     4321 gcggcagacg cggctccatg cgcacccacg tgcccgcctc ggccgtcgtc tcgtcctcgc
     4381 ccagcgtcag gcgggcgcgc ccgctcagga tgtggatgat ggctggcaca gcggcggtgt
     4441 gctcgctcaa ctcctgaccg ggcgcgaagc tgaagaccat cgcgctcagg cggtcgtcct
     4501 tataaaaggt acggctcaga atggcgtcgg cgggcacgtc cggggccagc gcggcaacgt
     4561 tgtcaacata gaaatagggt ttgtccatct cgctctctga gaagctgggc agaccggacg
     4621 ggcggcggtg ggtggggcgg agaggccccg tcgagtctgc ggttagtggg tgggcgcggg
     4681 cgggcttagc ggccggcggc gatgaccgcc agcaactcgt cggacaacgc ctgcggcggc
     4741 aggtcgtagg tcgtggccgc gtcctgcacc gtgcaaaacg gcgccagcgc gcagccgacg
     4801 caggccaggg ccaggccgtt gaagacctcg gccgtcgccg gccagcgcgc catgacctct
     4861 tctaccggca gttcgcgcag ccgctctgtc gtcatcggtg cgtccgtcgt catcgtcgtg
     4921 cccgctcgtc gcgggccggc cgccgcgcgg cggccggccc agaactatct gtctactcac
     4981 tcgtgcaatg tgcgaatgta agcgatgata tcggtcagct gttcatcggt caacgccggg
     5041 ttgccgccct tggggggcat gtctacgccg gtcgtgttgg ccgggtcgct gatcggccgg
     5101 cccgtcttga tgaagttcag cagctcgtgg tcatcgaccg tcagcaggaa ctcgctggtc
     5161 gtgaacggct tgcccaggcc ctcgatgccc tcgccgcccg gcccgtggca ggcgatgcaa
     5221 accatgtcga actggacctt gcccgcctcc gggtcgccgt gcagcgagcc gtcgtcgccg
     5281 gccggggctt cggccacggc gttgtcgttg gccggcgcgg ccgtctcttc gccacccccg
     5341 ccgccacagg cagccagagc caacaccaat gccagagcgg ccagaagggc caaaagcgtg
     5401 tacttgcgtg tcattaatct ctccttgttc ttgcgggcgc tctagtcgcc catctcgttg
     5461 tcgcccattc cggcgacgtt ttccaactcg cccgcctcgg cgcgcgcaag aatctcgctg
     5521 aagcgcatca cctcggccgc tgtctggtcg aaagccaacg cctgggccga ttgctcatcg
     5581 gccagcacgg caatgcccgt gcccatcggc gtgcgcagcc cggaattcat cacgaaccag
     5641 gcctcttcgg ccggcagcca ggcggcactg ttgacatcgt gcacccagac tgacgccgtc
     5701 gcctccgggt tcctggccag aaagaccagc atctcgccca cgtcgtcgaa gcggcgcgcc
     5761 tcgcccgcgg ccgtccagta ggccgcggcg tacttctcct cgctgatgat catgtcgcac
     5821 tcgtcgcaga catcttgacc atagaggatt tccggcggct gcgtcgtgtc cgccccgccg
     5881 ccacaggcag ccagggcgag cagtagtgcc agggcgagca ataaaacacc ggccgccggc
     5941 cggcggccga ccccctgccg aggggccagg ggccaggggc cagaggccag ggcaagagcg
     6001 cgccggctta ctgacctagc ggcccgctgc cctgctggcc actgacgacc gctcactctc
     6061 gtcatcacaa caccccccgc ccccgaaaca cgcgcagcga tagcgccagg g
//
LOCUS       DAOSMQ010000148         6100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13031_length_6100_cov_4.877254, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000148 DAOSMQ010000000
VERSION     DAOSMQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13031_length_6100_cov_4.877254"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..645)
                     /gene="rplT"
                     /locus_tag="PK829_06415"
     CDS             complement(292..645)
                     /gene="rplT"
                     /locus_tag="PK829_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013507016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOU40884.1"
                     /translation="MRVKGGFVTRRRHKKILDMTKGQWGSRSKLFRRANEAMMKSYFY
                     AYRDRRNRKRDFRRLWIARINAATRQHGLSYSNFIHGLKVANVRLDRKALADLAVRDE
                     TAFAAVIDIAKNALA"
     gene            complement(733..978)
                     /gene="rpmI"
                     /locus_tag="PK829_06420"
     CDS             complement(733..978)
                     /gene="rpmI"
                     /locus_tag="PK829_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOU40885.1"
                     /translation="MPKATKKYKLKTYKAAAKRFRVTGSGQIVRTKGGKSHLRRRKSK
                     RTKQMFSEMIVVSAPGDRRRIRRMAPYLKLYKANPPA"
     gene            complement(1111..1674)
                     /gene="infC"
                     /locus_tag="PK829_06425"
     CDS             complement(1111..1674)
                     /gene="infC"
                     /locus_tag="PK829_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOU40886.1"
                     /translation="MKRVRKPPVTRSDTVDYRINRQIRAREVRLIDHNGVNRGVLPFF
                     DALRIAEEAELDLVEVAPNANPPVCRVLDFGKFAYEQTKKEREARKNQTKVEIKTIRL
                     TPRTTGYHKDIGVRNARKWLEEGKKVKFMVRFKSREISYPEIGQRALQEIAEDLKDIA
                     IVEQQPTLEGWRMTLTLSPEALATQKT"
     gene            complement(1837..2412)
                     /locus_tag="PK829_06430"
     CDS             complement(1837..2412)
                     /locus_tag="PK829_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOU40887.1"
                     /translation="MTAKGTAANSNAWIWTLLRGVIALGLGIFLIVGGQTARTTAAYV
                     ILAYVAISGGMQTFSGLLNRRAPGSATDRIRGLVGLVGGIAMLLLLYFNVLTLSTTFI
                     IIAILVIAFGLLGLFEVFFDRGGAYFRWMPVLVNVLLVALGALVFYSSSREGFDLQLW
                     SGLVLVILGAAVGAYAYFAQKPSPQLATADL"
     gene            complement(2503..2715)
                     /locus_tag="PK829_06435"
     CDS             complement(2503..2715)
                     /locus_tag="PK829_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40888.1"
                     /translation="MAINTVPAAPATSTAPRAPRPAKISRSQAAAERIEDEYAYITDD
                     LRRVFILAALMFALLIVVNLVFTYIG"
     gene            complement(2850..3770)
                     /locus_tag="PK829_06440"
     CDS             complement(2850..3770)
                     /locus_tag="PK829_06440"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOU40889.1"
                     /translation="MSGKRAPEELNAAELEQLLIARRHAEREQRRRRAAAEGRIVVPD
                     ERRAAAADAAAVGTALPTPRPRPLWRWLADRALLLVELAAVVALVAIVASLWATNRQL
                     NRELAAAQAAQSQALALPTATATPAIGVVLLPGGHKPPVDGLPPEPGEAGGIPTHLLP
                     AVSAYVPPPIPTPGPEQARRLEIPALSGDYPIVQGDDWEQLKKGIGQYAGSGQPGRAG
                     NVVLSGHNDIYGEPFRYLDRLQVGDEIIVSTERQSYTYVVSEVRVVDPTDVWVMAPTE
                     AAQVTLISCYPYRVNTRRIVVFAQLTENRG"
     gene            complement(3953..>6100)
                     /locus_tag="PK829_06445"
     CDS             complement(3953..>6100)
                     /locus_tag="PK829_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40890.1"
                     /translation="FSRPWREPLSLSAAARARAVLVAILLLAFALRVWQLTSIPPGLT
                     HDEANHGREAIGILDGVLRYFFPLNYGSEPLYSYTVALSMAALGRGLFALRLVNVVFG
                     LAAVAISAAWAAPRLGRPAALLGAALLAVSFWPLASSREALRAGMLPFFMGLAAWAFW
                     RILEATPGRTNGERAGAIRAVWPHVLLFGLALAATLHIYLAARVGWLLFPLFLAYLAL
                     AHRADFRRAWPPALAGLLLGGLLSAPLFLYLRAHPEMQTRLDMLDGPLKAIAAGQWRP
                     VLANVANALLAFVWPGRGDTFLAYNIPGRPVFDAVSAALFVVGLGVCLGRWRRPAYAF
                     LLLWFAIGILPSLITGPTANTTRNLAALPAVFLIPAIGFVALMGRAWIDQEAAHAKAQ
                     RRKSHPRARYSSSRLSPFAPLREVFSPVTRHWSLVAAVLWLGFAGAVSARDYFARWAN
                     APEVRGAYQHTLAAALAHVAAVYPDAEPVVFSSVYPGAAHDPSIAFVLAAGRPALSRT
                     ARWADARWALALPPGVSLAVVPASTPPHPALAAWLAALATVELRPDDLDPRFTVYRLD
                     GDRASADLAAWAVPPVDFGGAVELVAARWLAAGVRPGETAELLTAWRVLDPARAGPAG
                     PPTFASDAVLFSHVLDGAGGRLSQHDSLDAPSWAWQAGDLFVQIHPLVVPASAAPGEY
                     AALVGFYDRATGVRLPASSGGDTATIPSLVIEE"
BASE COUNT         1105 a         1936 c         2096 g          963 t
ORIGIN      
        1 ggcgcgggaa gcgagacggg gcgggcggcc ggtggcggtc ttcactggcc gcgatgatac
       61 tgtctggccg cgtgggtcgc aactacgctt ggccgacgct cgccgtgcgc catagtgcct
      121 ggcactttcc tgagtgcctg gcactttctt gagtgcctgg cactttccgg tagatggcgc
      181 gctcgccaag aaggtgcgac gcaccgaaga aagtgcgacg cgccgggaaa atgaaaacgc
      241 cacacggacg agcgtgtggc gttgacgggt tatgcgaaaa ctaagaggga ctcaggccaa
      301 agcgttcttg gcgatatcga tcacggccgc aaacgccgtc tcatcgcgca cggccaggtc
      361 ggccagcgct ttgcgatcca gtcggacatt ggccaccttc aggccgtgga tgaagttgct
      421 gtagctcagc ccatgctgcc gcgtggccgc attgatgcgg gcgatccaca ggcggcgaaa
      481 atcgcgcttc cggttgcgcc ggtcgcgata ggcatagaag tacgacttca tcatggcctc
      541 gttggcgcgc cggaacagct tgctccgcga gccccattgg cccttggtca tgtcgaggat
      601 cttcttatga cgacgacggg ttacaaaacc accttttaca cgcattgtga cttacctcgc
      661 atttggtgtc gcctggtcac tcaaccccag gggcaaggcc aggcagcgat tgactgctcg
      721 attcgattcc acttaagccg ggggattggc cttgtacagc ttgagatagg gggccatccg
      781 cctgatccgg cggcgatcgc cgggggcgct gaccacgatc atctcggaga acatctgctt
      841 ggtgcgcttc gacttgcgcc ggcggagatg actcttgccg cccttggtgc ggacgatctg
      901 gcccgagccg gtgacgcgga agcgcttggc ggccgccttg taggtcttca gtttatattt
      961 ctttgttgcc tttggcatgg ttacatccct ttccacagtc tctgtcggcc gcggctcaac
     1021 tcgtcctgag cggctgccgg aaaaaaaggc cccgcagggc cttttcggtc taccgtctta
     1081 tgcggttgcc caacctgcgg ccgggcggct ctaggtcttc tgcgtcgcca gcgcttcggg
     1141 ggagagggtc agggtcattc gccagccttc cagcgtcggt tgctgttcga cgatggcgat
     1201 gtccttcagg tcttcggcga tctcctgcaa ggcccgctgg ccgatctccg ggtaagagat
     1261 ctcgcgggac ttgaagcgga ccatgaactt gacctttttg ccctcttcta gccacttgcg
     1321 cgcgttgcgc acgccaatat ctttatggta accggtggtg cgcggcgtca ggcggatggt
     1381 cttaatctcg accttcgtct gattcttccg cgcttcccgt tctttcttcg tctgttcgta
     1441 ggcgaacttg ccgaagtcca acacccggca gacgggcggg ttggcattcg gggctacctc
     1501 gaccaggtcc agttctgctt cctcggcgat gcgcagggca tcgaagaagg gcaaaacacc
     1561 gcgattgacg ccgttatgat cgatcaggcg cacttcacgc gcccggatct gccgattgat
     1621 acggtaatcg acggtgtcgg atcgtgtaac tggaggtttt cttactctct taatctattc
     1681 agtcctcttg gcgtgcattc tgacttgcca gaacgcccct actaatgcaa cagcaaaagc
     1741 ttaggcccgt gcctaagaca gccctatgat agcatagagc caacggcgcg tcaaacgcct
     1801 ccccgcagcg gggccaatgc agcggccgcg ccgcacctaa aggtcggctg tggccagttg
     1861 cggcgacggc ttctgggcga aataggcgta ggcccccaca gccgcgccca ggatcaccag
     1921 caccagccca ctccacagct gcaagtcaaa gccctcgcgc gaactggagt agaacaccag
     1981 cgcgcccaac gccaccagca acacattcac cagcaccggc atccagcgga agtaggcccc
     2041 gccccggtca aagaagactt cgaacagccc cagcagacca aaggcgatga ccaggatggc
     2101 gatgatgatg aacgtcgttg aaagcgtcag gacgttgaag tacagcagta gcagcatggc
     2161 gatgccaccg acgagaccaa ccagtccgcg gatgcggtca gtggctgagc caggcgcgcg
     2221 ccggttgagc aggccactga acgtctgcat cccgccgcta atggcaacat aagccaggat
     2281 gacataggcg gctgtcgttc gggccgtttg gccgccgacg atcagaaaga tgcccaggcc
     2341 gagggcgatg acgcctcgca gcagcgtcca aatccaggcg ttgctgttgg ctgcggttcc
     2401 ctttgcggtc atgataacct ccgttagatg gtgatggaac gatgaatggg aatcaacagg
     2461 ccccggcccg ccggtcacct gtgaccggtg tcggggcgct cgctagccga tgtaagtgaa
     2521 aaccagattg acgacaatga gcagagcgaa catcagcgcg gccaggataa acacccgccg
     2581 caggtcgtcg gtgatgtagg catattcgtc ttcgatccgc tcagcggcgg cctggctgcg
     2641 gcttattttg gccggacgcg gggcgcgcgg agcggtggag gtagccggcg cggccggcac
     2701 ggtgttgata gccatcccgt cgccttcgct cttggcgggg gcgggcttgg caggtctggc
     2761 ggtggatttc gccttcggtc gatttgtctt cttggccata atactcgatt catcctcaag
     2821 gcagagtata agaccgagga cgcgctttgt catcccctgt tttcggtcaa ctgggcaaag
     2881 acgacgatgc gccgcgtgtt gacacgatag gggtagcagg agatgagcgt cacctgggcc
     2941 gcctctgtcg gggccatcac ccacacgtcg gtcggatcga cgacgcgcac ttcgctgacg
     3001 acgtaggtgt agctctgccg ctcggtggaa acaatgatct cgtcgcccac ctgcaagcgg
     3061 tcaaggtagc ggaatggctc accatagatg tcgttgtggc cggagaggac gacattcccc
     3121 gcccgccccg gctggccgct gccggcgtac tggccgatgc ccttcttcag ttgctcccag
     3181 tcgtcgccct ggacgatggg gtaatcgccg ctgagggccg gaatttccag ccggcgggcc
     3241 tgctcggggc caggggtggg gatgggcggg ggcacgtagg cgctgacggc cggcagcagg
     3301 tgggtgggga tgccaccggc ctcgcccggc tccggcggca ggccgtccac gggcggcttg
     3361 tggccgccgg gcagcaggac gacgccgatg gccggcgtgg ccgtggccgt gggcaaggcc
     3421 agcgcctggc tctgggccgc ctgggccgcg gccaactcgc ggttgagttg ccggttggtg
     3481 gcccataggc tggcgacgat ggccaccagg gcgacgacag cggccagctc caccagcagc
     3541 agcgcccggt cggccagcca ccgccatagc ggccgcggcc ggggcgtggg cagcgccgtc
     3601 cccaccgcgg cagcatcggc ggccgcggcg cgacgctcgt cgggcacgac gatgcgcccc
     3661 tcggccgcgg cgcggcgccg gcgttgctcg cgttcggcgt gccgccgggc aatgagcaac
     3721 tgctccagtt cggcggcgtt caattcttcg ggcgctctct tgccagacat ggcgtgatca
     3781 tagtgtggga ggggggaaat tacgaattag gaattgggaa agaagagaag aacctcacgc
     3841 caggatcgca aagggcgcaa aaagaaatcg acgcaaggac gccaagaaaa cgcctctttg
     3901 cggtcttagc gcccctggcg ctcttggcgt gaggttcttt gcgtgagggc ctttactctt
     3961 caataaccag tgatggaatg gtcgcggtat cgccgccgga ggacgcgggc aggcggacgc
     4021 cggtggcgcg gtcgtagaag ccgacgaggg cggcgtactc ccccggcgcg gccgaggcgg
     4081 gcacgaccag cgggtgaatc tggacgaata gatcgcccgc ctgccaggcc cacgaagggg
     4141 catcgaggct gtcgtgctgg ctcagccggc cgcccgcgcc gtcgaggacg tggctgaaca
     4201 gcacggcgtc gctggcgaag gtcggcggcc cggccggccc cgcccgcgcc gggtcgagga
     4261 cgcgccaggc ggtcagcaac tcggccgtct cccccggccg gacgcccgca gccagccagc
     4321 gcgccgccac cagttcgacc gccccgccga agtccaccgg cggcacggcc cacgcagcca
     4381 ggtcggccga ggcccggtcg ccgtcgaggc ggtagacggt gaaacgcggg tcgaggtcgt
     4441 cgggacgcag ttcgaccgtc gccagcgccg ccagccacgc cgccagcgcc ggatggggcg
     4501 gcgtggaggc gggcacgacg gccagcgaca cacccggcgg caaggccagc gcccagcggg
     4561 catcggccca gcgcgccgtc cggctcagcg ccgggcggcc ggcagccagc acgaaggcga
     4621 tggacgggtc atgggccgcg ccggggtaga cgctggagaa aaccaccggc tcggcgtcgg
     4681 ggtagaccgc ggcgacgtgg gccagcgccg ccgcgagggt gtgctggtac gcgccgcgga
     4741 cttcgggcgc gttggcccag cgggcaaagt agtcgcgcgc cgagacagcg ccggcgaagc
     4801 caagccacag cacagccgca acgagtgacc agtggcgagt gacgggtgag aagacctcac
     4861 gcaaaggcgc aaaggggcta aggcgcgaag aagagtatct cgcgcgagga tgcgatttgc
     4921 gtctttgcgc ctttgcgtga gccgcttcct gatcaatcca ggctctaccc atgagggcga
     4981 cgaagccgat ggcgggaatg agaaacaccg ccggcagcgc ggccaggttg cgagttgtgt
     5041 tggccgtcgg gccggtgatc agcgacggca ggatgccgat ggcaaaccac aacaatagga
     5101 aggcataggc cggccgccgc cagcgcccca ggcagacgcc cagcccgacg acaaagagcg
     5161 ccgccgagac ggcgtcgaac accggccggc cggggatgtt gtaggccagg aaagtgtcgc
     5221 cgcggccggg ccaaacaaag gccagcagcg cgttggcgac gttggccagc accggtcgcc
     5281 attggcccgc ggcaatggcc ttcagcgggc cgtcgagcat gtctagccgc gtctgcatct
     5341 ccgggtgggc gcgcagatag aggaacagcg gcgcgctgag cagcccgccc agcagcagcc
     5401 cggccagcgc cggcggccac gcccgccgga agtcagcccg gtgggccagg gccagatagg
     5461 ccaggaagag cggaaagagc agccagccga cccgcgcggc caggtagatg tggagcgtgg
     5521 ctgccagagc cagcccgaac aggaggacgt ggggccagac agcgcggatc gcgccggccc
     5581 gctcgccgtt cgtccggccc ggtgtggcct ccaggatgcg ccagaaggcc catgccgcca
     5641 gccccatgaa gaacggcagc atgcccgccc gcagcgcctc gcggctgctg gccagcggcc
     5701 agaaggacac ggccagcagt gccgcgccca gcaaggccgc cggccgcccc agccgcggcg
     5761 cggcccaggc ggcgctgatg gccacggcgg ccagcccaaa gacgacgttg accagccgca
     5821 gggcgaacag gccgcgcccc aacgcggcca tgctgagagc gacggtgtag ctatagagcg
     5881 gctcgctgcc gtagttgagc gggaagaagt agcgcagcac gccatcgagg atgccgatgg
     5941 cctcgcggcc gtggttggcc tcgtcgtggg tcaggccggg cgggatgctg gtcagttgcc
     6001 agacgcgcag ggcaaaagcc agcagcagaa tggctaccaa caccgcgcga gcgcgcgcgg
     6061 cggctgacag agaaagaggc tcacgccaag gacgcgaaaa
//
LOCUS       DAOSMQ010000149         6094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13046_length_6094_cov_5.210796, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000149 DAOSMQ010000000
VERSION     DAOSMQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13046_length_6094_cov_5.210796"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PK829_06450"
     CDS             <1..648
                     /locus_tag="PK829_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU40891.1"
                     /translation="DGVELRDYPRRYLREQIGFVLQEPFLFSRTIRENIMYGVHREVT
                     DEDVYRAARAAAVHDVILTFPDGYDTVVGERGVTLSGGQKQRVTLARTLLKDPAILIL
                     DDATSSVDTETEAAIREALQELIPGRTTFIIAHRVQTVMNANLILVLEKGRIVQSGTH
                     AELLEQEGMYRRIYELQSQIEDELQQDLAAAHAATGSNGHYRTPERQSTGRAALD"
     gene            703..2505
                     /locus_tag="PK829_06455"
     CDS             703..2505
                     /locus_tag="PK829_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40892.1"
                     /translation="MEEEYFEEEDFNTRFSGRTVGRILQQVVPYWRMVVMFLGFVVLV
                     TISDSIFTYFGKLLVDDAIIPRDPAALRSILVYFGILSALTAGVVFGFIFITALLGEK
                     VRYDLRRKLFDHLQNLSFSYFDKTAVGWIMSRVTSDTERIADLVTWGLLDVTWGLTSV
                     LVATGFMLFINWRLALLVMITIPLLMGVAYYFRRLILTQFREVRRINSKITGAYNENI
                     SGVRVTKSLAREDKDLTEFQGLTGMMFNASYRANVLSAMFLPAVQLISALGVAMVIFY
                     GGLLSLRGETTVGQLQAFIGYVTFMIWPVQEMARVFAQMQQSIASGERVFSLLDAVPD
                     VQDKPGALETAHLEGDIVFDDVTFYYEKGKHVLDNFNLRVRPGETIALVGPTGGGKST
                     IVNMLCRFYEPKEGRVLFGEHDYTDLTQHAIQSRIGMVLQTPHLFSGSIRDNLRYGRL
                     DATDDELVEAARLAGAHDFIVTLENGYDEQVGEGGSKLSVGQKQLISLARAILADPDL
                     FIMDEATSSVDTLTEALIQRGMEQMMEGRTSFVIAHRLSTIKRASRILVIENGHIAEQ
                     GTHAELIRAGGHYYNLYTRQFRREREVALGITGD"
     gene            complement(2636..3145)
                     /locus_tag="PK829_06460"
     CDS             complement(2636..3145)
                     /locus_tag="PK829_06460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40893.1"
                     /translation="MPDILIRRATADDAAALVRLFSAPRAMWGTLQVPYTSEEARRRR
                     LVDAPEGTYPLVAEVDGEVVGQLTLHSNPTRPRRAHVGALGMAVRDDWHGRGVGTALM
                     AACLDLADNWLNLTRMELEVYTDNEPAIRLYTKFGFEIEGTMRRYAYRDGEWVDAYFM
                     ARLRDAPRP"
     gene            complement(3162..3674)
                     /locus_tag="PK829_06465"
     CDS             complement(3162..3674)
                     /locus_tag="PK829_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40894.1"
                     /translation="MPVIKRLLYVLGIVALTAAVVVAGLLAGAPAPAGAAPQITITAS
                     PTFTATLSLTADRAYVPVGETLTLTADLVARGCNYPIMDLTVKEDPARPPLFAHIDPP
                     GDIIGPGISFPSAWTFRAERAGTTRFLAQTFGETDCNGWQWHYENAVSPSIVVYDLPI
                     HVWLPTISSP"
     gene            4030..4641
                     /locus_tag="PK829_06470"
     CDS             4030..4641
                     /locus_tag="PK829_06470"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOU40895.1"
                     /translation="MARTRAFDESDALEQAMSLFWERGYEATSVRELAAGTGLSSSSL
                     YATFGSKHDLYLAALAQYRQVERDEMAGLLADPRAVREILAEVFARLIDRLVADPARR
                     GTFTLNAAVELGGQDEAVTAELRAHFDDIEALLGRRLAEAQAEREIAAHFPPGELARF
                     LLFGLYGLAMMVKVYPDRTQLERTAAVTLAVLDCQTKGAAIGR"
     gene            4785..>6094
                     /locus_tag="PK829_06475"
     CDS             4785..>6094
                     /locus_tag="PK829_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOU40896.1"
                     /translation="MDTTLPTPPVVSGAKPLLGHATEYGDGRGDLLRRGYEEHGKVFT
                     IKLGPQNVAVLIGPENQRTFFMETDKALNIATPYKFLRAAFGEVLFIAGHDEYLRQRP
                     FLTQAFRREKMAHYISIMGREVQKWLDALGDEGEFEVVGTMGWLAQQVAGSALMGDRF
                     QEEAGREFWDLYEDLSKGIDAVLPPNLPLPKFWRRDRARARMGAILKPILDERRAHPE
                     QYNDFLQDFVNSRYGDTDAPIEDEVLMNLMLGLMFAGHETTAGQAAWNVILLLQHPDY
                     LAIVREEIDRVAPAGTPIDGRTLHALTHMNYAVTEVERLYPSADILLRDVDEDIEAGG
                     YRIPAGWKVQIASEVAHQLPELFAAPEQYDPLRFAPERAEDKADRFALIGFGGGTHKC
                     MGMNFANNEIAVISTMLLQQFDLELATPRPTIARGVGANRPTPTL"
BASE COUNT         1050 a         1939 c         2101 g         1004 t
ORIGIN      
        1 gacggcgtgg aactgcgcga ctacccgcgc cgctacctgc gcgagcagat cggcttcgtc
       61 ttgcaagagc cgttcctgtt ctccaggacg atcagggaga acatcatgta cggcgtccac
      121 cgcgaggtga cggacgagga cgtctatcgc gccgcgcggg cggcggccgt ccacgatgtc
      181 atcctgacgt tccccgacgg ctatgacacc gttgtgggcg agcgcggcgt gacgctgagc
      241 ggcggccaga aacagcgcgt gacgctggcg cggacgctgc tgaaggaccc ggccatcctg
      301 atcctcgatg acgccacgtc gtcggtggac acggagaccg aggcggccat ccgcgaggcg
      361 ttgcaggaat tgattcccgg ccggacgacg ttcatcatcg cccaccgggt gcagacggtc
      421 atgaacgcga acctgatcct ggtgctggag aaggggcgca ttgtgcagag cggcacgcac
      481 gccgaactgc tggagcaaga gggtatgtac cggcgcatct acgagttgca gtcgcagatc
      541 gaagatgagc tacagcaaga cctggccgcg gcgcacgccg ccacgggcag caacggccac
      601 taccggacgc cggaacgcca gagcacgggc agggcggcgc tggactagtc gggcgcgacg
      661 cgccgagagg cgcgtcgccc cgctcgatgg cgagaggtca tcatggaaga agaatatttc
      721 gaagaagaag atttcaatac ccgcttcagt ggccggacgg tgggtcgcat cctgcaacag
      781 gtcgtgccct actggcgcat ggtggtgatg ttcctggggt tcgtggtgct cgtcaccatc
      841 tccgactcca tcttcaccta ctttggcaag ctgctggtcg atgacgccat catcccgcgc
      901 gacccggcgg cgttgcggtc gatcctggtc tacttcggca tcctgagcgc cctgacggcc
      961 ggcgtcgtct tcggcttcat cttcatcact gccctgctgg gcgagaaggt gcgctatgac
     1021 ttgcggcgca agctgttcga tcatttgcag aacctgagct tcagctactt cgacaagacg
     1081 gccgtcggct ggatcatgtc gcgagtcacc tcggacacgg agcgcatcgc cgacctggtg
     1141 acgtggggcc tgctcgacgt gacctggggc ctgaccagcg tcctggtggc cacgggcttc
     1201 atgctgttca tcaactggcg gctggccctg ctggtgatga tcaccatccc gttgctcatg
     1261 ggcgtggcct actacttccg gcggctgatc ctgacgcagt tccgcgaggt acggcgcatc
     1321 aactcgaaga tcaccggcgc gtacaacgag aacatcagcg gcgtgcgcgt caccaagtcc
     1381 ctggcccgtg aggacaagga cctgaccgag ttccaggggc tgacggggat gatgttcaac
     1441 gcctcctacc gggccaatgt gctctcggcg atgttcctgc cggcggtgca actcatcagc
     1501 gccctgggcg tggccatggt catcttctac ggcggtctgc tgtccctgcg tggcgagacg
     1561 accgttggcc agctgcaagc cttcatcggc tatgtgacct tcatgatctg gccggtgcag
     1621 gagatggccc gcgtctttgc ccagatgcag cagtcgatcg ccagcggcga gcgcgtcttc
     1681 tcgctgctcg acgccgtgcc ggacgtgcag gacaagcccg gcgcgctgga gacggctcac
     1741 ctggaaggcg acatcgtctt cgacgacgtg accttctact acgagaaggg caagcacgtg
     1801 ctggataact tcaacctgcg cgtgcggccg ggcgagacca tcgctctcgt tggcccgacg
     1861 ggcggcggca agtcgaccat cgtcaacatg ctgtgccgct tctacgagcc gaaggaaggg
     1921 cgggtgctgt ttggcgagca cgactacacc gacctgacgc agcacgccat ccagagccgg
     1981 atcggcatgg tgctccagac gccgcacctg ttcagcggca gcatccgcga caacctgcgc
     2041 tatggccgtc tggacgccac cgacgacgaa ctggtggagg cggcgcggct ggccggggcg
     2101 catgacttca tcgtcacgct ggagaatggc tacgacgagc aggtcggcga gggcggcagc
     2161 aagctgtccg tgggccagaa gcagctcatc agcctggcgc gggccatcct ggccgacccg
     2221 gacctgttca tcatggacga ggcgaccagc tcggtggaca cgctgaccga ggcgctcatc
     2281 cagcgcggca tggagcagat gatggaaggg cggacgagct tcgtcatcgc ccaccggctg
     2341 agcacgatca agcgggccag ccgcatcctg gtcattgaga acggccatat tgccgagcag
     2401 ggcacgcacg cggagttgat ccgcgccggc ggccactact acaacctgta cacgcgccag
     2461 ttccgccgcg agcgggaggt ggcgctgggg attacgggcg actagcccac cggagccagc
     2521 gcgcggcgtc gcaagacccg ctcaaggcca acaacgccgg gcaaggagag catcctggcc
     2581 cggcgttgtt cgttgccggc gggtggaaac cgagtttcct ggaggaactt ggtttctagg
     2641 gccggggcgc gtcgcgcagg cgggccatga aataggcatc cacccactcg ccgtcgcggt
     2701 aggcatagcg gcgcattgtg ccttcgatct caaagccgaa cttcgtatag agacggatgg
     2761 ccggctcgtt gtcggtgtag acctccagtt ccatgcgcgt caggttgagc cagttgtcgg
     2821 ccaggtcgag gcaggcggcc atgagcgccg tgcccacgcc gcggccgtgc cagtcgtcgc
     2881 gcacggccat gcccagcgcg ccaacgtggg cgcggcgcgg ccgggtgggg ttgctgtgca
     2941 gcgtcaactg gccgacgacc tcgccatcga cctcggccac cagcgggtac gtcccctccg
     3001 gcgcgtcgac caggcggcgg cggcgggcct cctcgctggt gtagggcact tgcagcgtgc
     3061 cccacatcgc ccgcggggcg ctgaagaggc gcacgagggc ggcggcgtcg tcggcggtgg
     3121 cgcggcggat gaggatgtcg ggcatggggg tttctccact tttacgggct actaatcgtt
     3181 ggcaaccaca cgtgaatggg cagatcatag accacaatag acggcgagac ggcgttctcg
     3241 tagtgccact gccagccgtt gcagtcggtc tcgccaaagg tctgcgccag gaagcgggtc
     3301 gtgcccgcgc gctcggcgcg gaacgtccac gccgagggga agctgatgcc tggcccaatg
     3361 atgtcgccgg gtgggtcgat gtgggcgaag agcggcggcc gggcggggtc ttccttcacg
     3421 gttaaatcca tgatcgggta gttgcagccg cgggcgacca gatcggccgt cagcgtcagc
     3481 gtctcgccca cgggcacata ggcccggtcg gcggtcaggc tgagcgtggc cgtgaacgtc
     3541 ggtgaggcgg tgatggttat ctgcggagcc gccccggcgg gcgctggcgc gccggccagc
     3601 aggcccgcga cgacaacggc ggccgtcagc gcgacgatgc ccaggacgta gagcaagcgc
     3661 ttgatcactg gcatgatctt ctcccggcgg tggccgcggc ccaggccaac ggccgcgccc
     3721 cgtcggctac agtatagcat agcgggggaa gtgggtcagt gccgggcttt gccaaagtcg
     3781 tcgagcgacc attgacctga caggtgagcg gccagtcgtt ctctggtgca agtacgattc
     3841 gtggccgctc acctgtcagg tcggtgcgcc tgggatgact ttggcaaagc tccgaggagc
     3901 caggggttgg aaagagagcg ttcccgagac cctggcccct ggaacctggc ccctgccttc
     3961 tagatgagtc tccgctggga agcgggcggc ataatggatc gctcgttcca gatatgctat
     4021 agtggggtta tggcgcgaac acgagcattt gacgagagcg acgccctgga gcaggcgatg
     4081 agtctgttct gggagcgcgg ctatgaggct acctcggtgc gcgaactggc cgccggcacg
     4141 ggcctgagca gcagcagtct ctacgccacc ttcggcagca agcacgacct ctatctggcc
     4201 gcgctggccc agtaccggca ggtggagcgc gacgagatgg ctggcctgct ggccgacccg
     4261 cgcgccgtgc gcgaaattct ggccgaggtg ttcgcccggc tcatcgaccg gctggtggcc
     4321 gacccggccc ggcgggggac gttcacgctg aacgccgctg tggagctggg cgggcaggac
     4381 gaggccgtga cggccgagtt gcgcgcccac ttcgacgaca tcgaggcgct gctcggccgc
     4441 cgtctggccg aggcccaggc cgagcgcgag atcgccgccc actttccacc cggggagttg
     4501 gcccgctttc tgctcttcgg gctgtacggg ctggcgatga tggtcaaggt ctacccggat
     4561 cggacgcagt tggagcggac ggcggcggtg acgctggccg tgttggattg ccagacgaag
     4621 ggggccgcca tcggccggta agcggctgat gggcatcggc gcgatgtggg ggagcgacta
     4681 cccacgacgc ctaacagttg agtcatgaat catggcgaca cttgcccgtg caggttgcgc
     4741 ggctgcggtt cgccccggaa aagcaatgga gtgaagtgaa tacgatggat actaccctac
     4801 ccacaccccc ggttgtgtcc ggggccaaac cgctgttggg ccatgccacg gagtatggcg
     4861 atgggcgcgg cgatctgttg cggcgcggct atgaggaaca cggcaaggtc ttcacgatca
     4921 agctcggccc gcaaaacgtg gccgtgctca ttgggccgga aaaccagcgc acgttcttca
     4981 tggagaccga caaggcgctc aacatcgcca cgccctataa gttcctgcgg gcggcctttg
     5041 gcgaagtgct cttcatcgcc gggcacgacg agtatctgcg ccagcggccg ttcctgaccc
     5101 aggccttccg gcgcgagaag atggcccact acatcagcat catgggccgc gaggtgcaga
     5161 agtggctcga cgcgctgggc gacgagggcg agttcgaggt cgtcgggacg atgggctggc
     5221 tggcgcagca ggtcgccggc agcgccctga tgggcgaccg ctttcaggaa gaagccgggc
     5281 gcgaattctg ggacctgtac gaggacctca gcaagggcat cgacgctgtg ctgccgccca
     5341 atttgccgct gcccaagttc tggcggcgcg accgcgcccg cgcgcgcatg ggcgcgatcc
     5401 tgaagcccat cctggacgaa cgccgcgccc acccggagca gtacaacgac ttcctgcagg
     5461 acttcgtcaa ttcgcgctac ggcgacaccg acgcgcccat cgaggacgag gtgctcatga
     5521 acctgatgct cggcctgatg ttcgccgggc acgagacgac ggccgggcag gcggcctgga
     5581 acgtcatcct gctgctgcaa caccccgact atcttgccat cgtccgcgag gagatcgacc
     5641 gcgtggcccc cgccggcacg cccatcgacg gccgcacgct ccacgcgctg acgcacatga
     5701 actatgccgt cactgaggta gagcggctgt acccgtcggc cgacatcctg ttgcgcgacg
     5761 tggatgagga catcgaggcc ggcggctacc gcatccccgc gggctggaag gtgcagatcg
     5821 cctccgaggt cgcccaccag ctgccggagc tattcgccgc gccggagcag tatgacccgt
     5881 tgcgcttcgc gccggagcgg gccgaggaca aggccgaccg cttcgcgctg atcggtttcg
     5941 gcggcggcac gcacaagtgc atgggcatga acttcgccaa caacgagatc gccgtcatct
     6001 cgaccatgct tttgcagcag ttcgacctgg agttggcgac gccgcggccc accatcgccc
     6061 gcggcgtggg cgcgaaccgg ccgacgccga cgct
//
LOCUS       DAOSMQ010000150         6093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13053_length_6093_cov_4.654853, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000150 DAOSMQ010000000
VERSION     DAOSMQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13053_length_6093_cov_4.654853"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PK829_06480"
     CDS             <1..1299
                     /locus_tag="PK829_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU40897.1"
                     /translation="LSAISRTNQRELLNLWQTGAGGEELQGLLISTAESANVRILVVD
                     SGAQQIIFDSVGEDDWVGDPLTQVERPQSLTMANVGRGSIFGTFVHSNGERWLVFAEP
                     NPAFDPVLIFYAEPEPTAGKFFSDFFLRPLLYAGGVAFLLAILLAAWLTGSVSRPLQH
                     LAAAAGAIARGDYKQRVPPEGPDEVRGVAESFNRMAAQVDATQTAQRDLVANVSHDLK
                     TPLTAISGWSQALLDGAADTPEDRRRAAETINTEAGRMARMVNELLDLARIESGQLHL
                     TLRPVDVSAVMADVHRGQVPRARARQIDLTLDAPSVPPVLGDPDRLAQVFTNLADNAL
                     TYTPSGGHVHLAVRADDGFVEGSVTDNGPGIPAEELPRVFERFYRLDKSRVRAEDGRR
                     GSGLGLAIVKELVAAQGGAVSVSSEPGRGSAFLVRLPLTP"
     gene            complement(1468..2568)
                     /locus_tag="PK829_06485"
     CDS             complement(1468..2568)
                     /locus_tag="PK829_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HOU40898.1"
                     /translation="MKAIRIHECGGPEVLQLDEMEVPQPGPGEVRVMIRAAGVNFIDI
                     YQRKGQYKGELPFTPGSEAAGEVHGLGEGVTGLEVGEPVAYAMHLGAYADFAIVPAWK
                     LVRIPPGVNTMTAAAAMLQGMTAHYLTHDTYRICPGDTVLIHAAAGGTGALVVQMAKL
                     RGARVIGTVSTAEKEQLARERGADEIIRYTEQDFEAEVKRLTDGRGVQVVYDSVGKDT
                     FLKGLNCLRPRGMMVLFGQSSGPVAPIDPQVLNHKGALFLTRPALAHYNTTHEEIRSR
                     ANAVFEWINSGQLVVRIDKTFPLDEAAGAHRYMEARQSKGKVLLGTQQGRAKKLEQLD
                     RTIDRTDLVDESAWESFPASDPPTFAASTNAG"
     gene            2989..4224
                     /locus_tag="PK829_06490"
     CDS             2989..4224
                     /locus_tag="PK829_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOU40899.1"
                     /translation="MSTRRVKLFPLIAAVIMLTLILSGRAVRPALADGDIVPDHYIVQ
                     LAPNVSANAKVSAVAADLGRAYGLRVSQVYDVAIIGFAARIPASRLQRLMADPRVLSV
                     EPDRVVTIQAQTLPTGIDRIDGELSSTISGNGSGAVNVDVAVLDTGIQTNHPDLNVVG
                     GKNCSTGNSYSDGHGHGTHVAGTIAAKDDGNGVVGVAPGARLWAVRVLNNQGSGTTST
                     IICGINWVTANAGTIEVVNMSLGGSGSDSTCPGSDAYHNAICNSVNAGVTYVVAAGNS
                     AANANNFRPATYSEVITVSALADFNGQPGGGGAATCRSDVDDTLADFSNYGSDVDLIA
                     PGVCIYSTYRGSTYATMSGTSMASPHVAGAAALYKSNNPGATPAQVKAALIAAANSNW
                     NNAGDPDGIKEPLLNVDGL"
     gene            4522..4968
                     /locus_tag="PK829_06495"
     CDS             4522..4968
                     /locus_tag="PK829_06495"
                     /inference="COORDINATES: protein motif:HMM:NF039927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HOU40900.1"
                     /translation="MSHHCPECGAAWADGRTCADDFHQLLFWENERPELGEVHHLMVL
                     CYHLQHPSLYSAEGLAHARRLLASFVADDLSPAEMRQRQRAAVDSGQRGWSVTARPGN
                     RGAYERTPEWTMRARDVVAGGIEQYRGSVRRWAAGIYDALERDVEG"
     gene            4971..5510
                     /locus_tag="PK829_06500"
     CDS             4971..5510
                     /locus_tag="PK829_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU40901.1"
                     /translation="MELPVCSHCGFTNRAGSRFCTQCGQALDAAVAMELDRVPETDPT
                     ASATAPTLAEAWGSPFPATPDVQVLPSRPGCVTIYAVLLFCGAAANTIIGLSLLRTGR
                     MSGLIFLGLAIAGVVVVTGLFRQKNWARIGVILLMCISIAGVATQALAGNINPYTLAA
                     LLVPASIIWWFATNEQYFA"
     gene            5587..>6093
                     /locus_tag="PK829_06505"
     CDS             5587..>6093
                     /locus_tag="PK829_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU40902.1"
                     /translation="MAKFLRANRKKLVGLSQIALSIVLLVWLLSRVGLGEVVATLSRI
                     NWYWYGLAFLLFQLNVVIRAFRWYVLLRALNDEPRFGYLVYLYYLGFFANNFIPSGFG
                     GDLVKVVTLRQSHGHGSEALSSVLMERVTGLVGSSLIAIGALLWNTSAHAADVDLPLA
                     LWALILVTA"
BASE COUNT         1008 a         2019 c         1944 g         1122 t
ORIGIN      
        1 ctctcggcca tcagccgcac caaccaacgc gaactgctga acctatggca gacgggcgcg
       61 ggcggcgagg agttgcaagg gttgctcatt tcgacagccg agagcgccaa tgtgcgcatc
      121 ctcgtcgtcg atagcggcgc gcagcagatc atcttcgact ccgtgggaga ggatgattgg
      181 gtgggcgatc cactgaccca ggtggagcgg ccgcaaagcc tgacgatggc caacgtcggc
      241 cggggcagca tctttggcac gttcgtccac agcaatggcg agcgctggct ggtcttcgcc
      301 gagccgaacc ccgccttcga tcccgtactc atcttctacg ccgagccaga accgacggcg
      361 ggcaagttct tcagcgactt tttcctgcgc ccgctgctct atgccggcgg cgtggccttc
      421 ctgctggcta tcttgctggc ggcgtggctg accggctccg tcagccggcc gctgcaacat
      481 ctggccgcgg ctgccggggc catcgcccgc ggcgattaca agcagcgcgt gccgccggag
      541 gggccggatg aggtgcgcgg cgtggccgag agcttcaacc gcatggccgc gcaggtggac
      601 gccacccaga cggcccagcg cgacctggtg gccaacgtct cccacgacct caagacgccg
      661 ctgacggcca tctccggctg gagccaggcg ctgcttgacg gcgcggctga cacgcctgag
      721 gaccgccgcc gcgccgccga aaccatcaac accgaagccg ggcgcatggc tcgcatggtc
      781 aatgaactgc ttgacctggc ccgcatcgaa tcgggccaac tccacttgac gttgcgcccg
      841 gttgacgtga gcgccgtaat ggccgacgtc caccgcgggc aagtgccccg ggcgcgcgcc
      901 cggcagatcg atctgaccct cgatgcgccg tctgtgccgc cggtgctggg cgaccccgac
      961 cggctggcgc aggtgtttac caacctggcc gacaacgcgc taacctatac gccctccggc
     1021 ggccacgtcc atctggccgt ccgcgccgat gacggcttcg tcgagggcag tgtcaccgac
     1081 aacgggccgg gcatccccgc ggaggagttg ccgcgggtgt tcgagcgctt ctaccggctg
     1141 gataagtcgc gcgtgcgggc cgaggacggc cggcgcggct ccgggctggg actggccatc
     1201 gtcaaagaac tcgtcgccgc ccagggcggc gcggtgagcg tgtccagcga gccggggcgc
     1261 gggtcggcct tcctggtgcg gttgccgctg acgccgtagc tctgaaagcg ctcacgcaaa
     1321 ggcgctcacg caaaggcgca aagtaagcaa aggacgcaaa gaagaatctc tcttcaattc
     1381 ttctttgcgc ctttgcccct tagcgacttt gcgtgagcgc cttcgcgtga gcgccttctt
     1441 ccgacgctgc tatctccctg attaccgcta accggcgttg gttgaagcgg caaaggtggg
     1501 cgggtcgctg gccgggaacg actcccaggc cgattcgtcc accaggtctg tgcggtcgat
     1561 tgtccggtcg agttgttcaa gcttcttggc ccggccctgc tgtgtgccca atagcacctt
     1621 gcccttcgac tggcgcgcct ccatgtagcg gtgggcgcct gcggcttcat ccagcgggaa
     1681 agtcttgtcg atgcgcacaa ccagctggcc gctgttgatc cactcgaaga cggcgttggc
     1741 gcggctgcga atttcctcgt gggtggtgtt gtagtgggcc agggccggcc gggtcaggaa
     1801 gagcgcgccc ttatgattca gcacttgcgg atcaattggc gcaaccgggc cgctcgactg
     1861 gccgaagaga accatcatgc cccgcggacg taggcagttc aaccccttca ggaacgtgtc
     1921 cttgccgaca gagtcgtaga cgacctggac gccgcggcca tcggtcagcc gcttcacctc
     1981 ggcctcgaag tcctgctcgg tgtagcgaat gatctcgtct gcgccgcgct cgcgggccag
     2041 ttgctccttc tccgcggtcg agaccgtgcc aatgacccgc gccccgcgca gcttggccat
     2101 ctgtaccacc agcgcgcccg tgccaccagc ggcggcgtgg atgagcaccg tgtcgccggg
     2161 gcagatgcgg taggtgtcgt gggtcagata gtgggcggtc atgccctgga gcatcgccgc
     2221 ggccgcggtc atcgtgttga cgccaggcgg gatgcggacg agcttccagg cgggcacgat
     2281 ggcgaagtcg gcgtaggccc ccaggtgcat ggcataggcc accggctcgc ccacctccag
     2341 cccggtgacg ccctcgccca acccgtggac ttcgccggcg gcctccgagc cgggcgtgaa
     2401 gggcagctcg cccttatact gacccttgcg ctgatagatg tcgatgaagt tgactccggc
     2461 ggcgcggatc atcacccgca cctcgcctgg ccccggctgt ggtacctcca tctcatctag
     2521 ttgcagcact tccggcccgc cacattcatg gatgcgtatt gctttcatcg tttgcctcgt
     2581 ttgcagatgt cgcgccctgt aggatgtgga agagtcgccg ttggcgcagg gtcgccaatg
     2641 gcgtaacagg acaacgccga tgtgtctgtc acccgcggct ggctgagcag cgcggccatt
     2701 tggctatagg gtaatgcttt gcgccggaat tgtcgccttt ctggcgacat aggcaagata
     2761 tagaaagggg tatgaatacg cttcccggcc gcggcgacag ttgagggtca tttgcgtggc
     2821 atttccgtgc ccagtgaaga agccatttgc gcttccaaca actcttttta taatgccctc
     2881 gctcgccccc aaaggacaag tcccacggga agtagtggag tggccgcgcg gctgcttagt
     2941 ggtcaggtct gttaaaaacc aaatcgcgtt gaaggagagt gtccgatcat gtcaacccgc
     3001 agagtcaagt tgttcccgct catcgccgct gtcatcatgc tcactctcat cctgagtggc
     3061 cgcgccgtcc gtccggcgct ggccgatggc gacatcgtgc ccgaccacta catcgttcaa
     3121 ctggccccca atgtcagcgc caacgccaaa gtcagcgccg tggccgctga tctcgggcgg
     3181 gcctatggcc tgcgcgtctc ccaggtttac gatgttgcca ttatcggctt cgccgcgcgt
     3241 atccccgcgt cgcgcttgca gcggctgatg gccgacccgc gcgtgctgtc ggttgaaccc
     3301 gaccgcgtcg tcaccatcca ggcccagacg ctgcccacag gcattgaccg catcgatggc
     3361 gaactgagca gcaccatctc cggcaacggc tccggcgcgg tgaacgtgga tgtggctgtg
     3421 cttgacacgg gtatccagac caatcacccc gatctgaacg ttgtcggcgg caagaactgc
     3481 tccaccggca actcctacag cgacggccac ggtcacggca cgcacgtcgc cggcaccatc
     3541 gccgccaagg atgacggcaa cggcgtcgtc ggcgtcgcgc ccggagcgcg gttgtgggcg
     3601 gtgcgggtgc tgaataacca gggctccggc acgacctcga cgatcatctg cggcatcaac
     3661 tgggtaacgg ccaatgccgg cacgattgag gtggtcaaca tgagcctggg cggcagcggc
     3721 tccgacagca cctgccccgg ttcagacgcc taccacaatg cgatctgcaa ttcggtcaac
     3781 gcgggcgtaa cctacgtggt tgccgccggc aactccgcgg ccaacgccaa caacttccga
     3841 ccggccacct attccgaagt catcaccgtc tcggccctgg ccgacttcaa cgggcagccg
     3901 ggcgggggtg gggcggccac ctgccgcagc gacgtggacg acacgctggc cgacttcagc
     3961 aactacggtt cggacgtaga cctgatcgcc cccggcgtct gcatctactc gacctaccgc
     4021 ggcagcacct acgccaccat gtcgggcacg tccatggctt cgccccacgt ggccggcgcg
     4081 gcggcgctct acaagtccaa caaccccggc gcgaccccgg cccaggtgaa ggcggcgctc
     4141 atcgccgcgg ccaactccaa ttggaacaac gccggcgacc cggacggcat caaggagccg
     4201 ttgctgaacg tggatggctt gtaggcgcgg ccccgcccgc gggcatgacg tagcactggc
     4261 caacaaatga agaggccggc gttccggtaa accggacgcc ggccctttgc gtcggatggg
     4321 caggccaccg catgaaccgt ggcacgcagg gtcatcgcaa agtcattaag acacctcacg
     4381 caaagagcgc caagggggca aaggacgcaa aaaaggcact tcttggcgtt cttagcttcc
     4441 ttcgcgccct ttgcgtgagg tcttcttccg actgtgcaat agcccgaccg gggcgcgggg
     4501 cagccgtggt acaatgggcg catgagtcac cactgtcctg aatgcggcgc agcgtgggcc
     4561 gatgggcgta cttgcgccga cgacttccac caactgctct tctgggaaaa cgagcggccg
     4621 gaactgggcg aggtacacca cctcatggtg ctgtgctacc acttgcagca ccccagcctc
     4681 tactcggccg aggggctggc ccacgcgcgg cgactgctgg ccagctttgt ggccgatgat
     4741 ctgtcgccgg ccgaaatgcg gcagcggcag cgcgccgcgg tcgattccgg ccagcggggg
     4801 tggagcgtta ccgcccggcc ggggaaccgc ggggcgtatg agcggacgcc ggagtggacg
     4861 atgcgggcgc gggatgtggt ggccgggggg attgagcagt atcgcggctc ggtgcgccgg
     4921 tgggcggccg gcatctacga tgcgctggag agggacgtag agggatagag atggaactcc
     4981 cagtttgctc ccattgtggc ttcacaaacc gagccggctc acgtttctgt acgcagtgcg
     5041 gccaggcgct ggacgctgcg gtcgcgatgg agctggacag ggtaccggag actgatccga
     5101 ccgctagcgc caccgcgccc acattggccg aagcgtgggg ttcgcccttt ccagccaccc
     5161 cggatgtcca ggttctcccc agccggcccg gctgcgtgac tatctatgcc gtccttctct
     5221 tttgcggcgc cgccgccaat accatcatcg gactatcctt gctgagaacc ggccgcatga
     5281 gcggtcttat ctttctgggc ctggctatcg ccggggttgt cgtcgtaacc gggttatttc
     5341 gccagaagaa ctgggcgaga atcggcgtca tacttctgat gtgtatttcc atcgccgggg
     5401 tcgccacaca ggcgcttgcc gggaatataa acccatacac tctcgcggct ttgctcgtgc
     5461 ccgcctcgat tatctggtgg ttcgccacga acgagcagta ttttgcctag cacggtgtgg
     5521 tgaccccagg ctccacgttg cgccgttcgc caacgagcgt cctgacgaga tataatctcc
     5581 tttgctatgg ccaaattcct ccgcgctaac cgcaagaagc tggttggcct ctcccaaatt
     5641 gccctcagca tcgtcctcct cgtctggctg ctcagccggg tggggttggg cgaggtggtg
     5701 gcgacgctgt cgcgcatcaa ctggtactgg tatggcctgg ccttcctgct gttccagctc
     5761 aacgtcgtca tccgcgcctt tcgctggtac gttctgctgc gcgccctgaa cgacgagccg
     5821 cgcttcggct atctggtcta cctctactac ctgggcttct tcgccaacaa cttcatcccc
     5881 tccggctttg gcggcgatct ggtcaaggtg gttacactgc gccagagcca cggccacggc
     5941 tccgaggcgc tcagttcggt gctgatggag cgcgtcaccg gcctggtcgg ctcgtcgctc
     6001 atcgccatcg gcgccctgct gtggaacacc tcggcccacg ccgccgacgt ggacctgccg
     6061 ttggccctgt gggcgctcat cctggtgacg gcg
//
LOCUS       DAOSMQ010000151         6088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13071_length_6088_cov_4.606166, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000151 DAOSMQ010000000
VERSION     DAOSMQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13071_length_6088_cov_4.606166"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK829_06510"
     CDS             complement(<1..345)
                     /locus_tag="PK829_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40903.1"
                     /translation="MKRTAYRFLWPLVALVLLWGAPVAMAQTGAAVVRPDPASLTVVA
                     GQMQTISVLVENAAEVYGIDIRASFDPALIEIVDDDPARDGVQLTPGTIPQPDFVAVN
                     AADNAAGTLRYAT"
     gene            complement(427..1962)
                     /locus_tag="PK829_06515"
     CDS             complement(427..1962)
                     /locus_tag="PK829_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOU40904.1"
                     /translation="MSRHVARLLVFALLLVALLAPIGRLAAVGPIVTPGTIPTAQIKG
                     VVKFRNFAPLATGFDVQIGPSEAVSANNGGDANYPWGAVNAVSFAYNGNQLAAGFPTL
                     NGGSPNTVTYPGALSGPLNYLQIDVVCRDPGATVALKNVMVAGTSLGDFMPATCGGAG
                     DTHSWYVTGLDLSAGFQLTGAIHLSGFAGLSGQETSKVLIRVGTIPATPATAAVTFTP
                     AAKALVCNATELRIDFRDMPAIYGYQFKVQYDEALLDAVGAFDNSWFNTTNQANVPGG
                     WGASCAAGVCKFAVSKVNPATPVSGSGPVGKITFTAQAAGVSMVQLTNLIVTDLDGFS
                     VPAAAPLPVELTTCGRASISGKVSLQGRVTPIDGGEVRAIDAGGVFPTVTVPFDAAGN
                     YTLSNLPVLPGGSSYKLRATHILYLGNEKTLALSPGAALTNQNTRLLGGDADNSGLNP
                     PGTVGVDVGDLSCVAGAFDGPPLSCGPFPLSSTDINKDNVTNIQDLAITGGNYARNPF
                     QPW"
     gene            complement(2078..4327)
                     /locus_tag="PK829_06520"
     CDS             complement(2078..4327)
                     /locus_tag="PK829_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOU40905.1"
                     /translation="MNTSGWFQGLDNGSYYGTIARVPSGTNGIVSAEGVAHAVITGTP
                     GEGAPFTRFGSYSSVWPGAWTAEIDVYLDPAWPAGTGFDYSVAASNSSGGYLRDFIFL
                     VAKDTSTGKLLVAASNGSGGAPREDLETLNHVEVTTAGWYTLRHVFYKSSTQLAVDLK
                     LVNSSGTVLFTQTLTDAADTLPGIVGGNRYGWFVYVANGQTLPIDNSRTCLGTQGCDP
                     NVVMVVAPTDGTMVMPTNVCAGGVLSLWTRDWSTCDENSTGTTGYVALAAGPATPPAG
                     IGSAQFVTAGPANGPAIFNPFELGLPLDQIFQLTYDTYGASSERPNFFFNVDYDLTDA
                     SQAWQGRMVWVPSLPGCATLVNNTWQTLNPLALRDAACWFQTQPPVVNNVVGAPLYPL
                     NGAAGSWNQLMAAYPEAGVHPTYGAIGFKVGSGSSAMTVSLDKLVVGYNNGADRFVTY
                     DFEPGAALNVSATPNNVCSTSQVKIDFANVPGAYGYEFVVDYNQTLVDALGAFSPALF
                     DPAKGSSPGTAWAANCTTTPGQCKFALTLHNPAPAVSGSGTVATINLTAKKAGVFALG
                     IHDVKLTDEDGVAISVTLPPAAIAVDVCGTATISGRVSLQGRVAPIDAGTVTITPRGG
                     GTPVSAPFSAVDGTYSLTVLYPPGGATFVLQAAHQLYLTNKKDLVVSGTAITGQNTRL
                     LGGDANNNAAVEIGDLSCIGGDFGLTGAAISDCGGLGSPDINKDAKVNIQDLSLAGGN
                     YDKVAPQGW"
     gene            complement(4993..5883)
                     /locus_tag="PK829_06525"
     CDS             complement(4993..5883)
                     /locus_tag="PK829_06525"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HOU40906.1"
                     /translation="MSEQTGFQLIIATFDDEATAAQAVNRLLQTFRGEYDNMPAAAYV
                     GKDASGQLTIRETADLGGKQGAAAGALAGGLVGLLSRRRGMFSSAALGALLGGVAAKK
                     LDTGIPDPRLEAIGRALDAASGAAVAIVSDNAVDQLLGVVKGLGAHVQAEPFARETDF
                     MKQLQAGNIAGAATILANQAEGRLAGATAMAVERAEALGGQARGMMTGDEPDTPTDEE
                     QIEILIDPDAAATMVSTDTQMTPGIPVRPDEEMKAAPAPKPAQVAGAGAVPDSDAGGL
                     ARTDIDESAAGVADIWPPAV"
BASE COUNT          983 a         1883 c         2195 g         1027 t
ORIGIN      
        1 ggtggcgtaa cgcagcgtcc cggcggcgtt gtcggcggcg ttgacggcca caaagtcggg
       61 ctgggggata gtgccgggcg tcagctgcac gccgtcgcgg gcggggtcgt cgtcaacgat
      121 ctcgatcagc gccgggtcga agctggcgcg gatgtcgatg ccgtagactt cggcggcgtt
      181 ctccaccagt acgctgatcg tttgcatctg gccggcgacg acagtgagcg acgccgggtc
      241 gggccgcacg acggccgcgc ccgtttgggc catggcgaca ggcgcgcccc aaagcagaac
      301 gagcgccacg agcggccaga ggaatcggta agcggttctt ttcatagtga caagaaatgg
      361 ggcggtacgc tgaccgcccc gcgaatgggg ggcgacccgc gagccgcccc ttgcgccgat
      421 ggaaaattac cagggctgga acgggttcct ggcgtagttg ccgccggtga tggccaggtc
      481 ctggatgttg gtgacgttgt ccttgttgat gtcggtgctg ctgaggggga acgggccaca
      541 gctgaggggc gggccgtcga acgccccggc gacgcagctc aagtcgccca cgtccacgcc
      601 gaccgtgccc ggcggattga ggccgctgtt gtcggcgtcg ccgcccagca gccgcgtgtt
      661 ctggttggtc agggccgcgc ccgggctaag ggccagcgtc ttctcgttgc cgaggtagag
      721 gatgtgggtc gctcgcagct tgtaggacga gccgccgggc agcacaggca gattgctgag
      781 cgtgtagttg cccgcggcgt cgaacggcac ggtgacggtc gggaagacgc caccggcgtc
      841 gatggccctg acctcaccgc cgtcgatggg cgtgacgcgc ccttgcaggc tgaccttgcc
      901 gctgatggag gcgcggccgc aggtggtcag ttcaaccggc agcggcgcgg cggccggcac
      961 gctgaagccg tcgaggtcgg tgacgatcaa attcgtcaac tgaaccatag agacgcccgc
     1021 ggcctgggcg gtgaaggtga tcttgcccac cgggccagag ccgctaacgg gcgtggccgg
     1081 gttgaccttg ctgacggcga atttgcacac gccggcggcg cacgacgcgc cccagccgcc
     1141 gggcacgttg gcctggttgg tggtgttgaa ccagctattg tcgaacgcgc cgacggcgtc
     1201 caacagtgcc tcgtcgtatt gcaccttgaa ctggtagccg tagatagccg gcatgtcgcg
     1261 gaagtcgatg cgcaattcgg ttgcattgca caccagcgcc ttagccgcgg gcgtgaaggt
     1321 gacggcggcc gtggccgggg tcgcgggaat ggtgccgacg cggatgagca ccttgctggt
     1381 ctcctggccc gacaggccgg cgaagccgct caggtggatg gcccccgtca actggaagcc
     1441 ggcgctcaga tcgaggccgg tgacgtacca gctgtgcgtg tcgccggccc cgccgcaagt
     1501 ggccggcatg aagtcgccca gcgatgtccc ggcgaccatg acgttcttga gggcaacggt
     1561 cgctccaggg tcgcggcaaa cgacgtcgat ttgcagatag ttgagcgggc cgctcagcgc
     1621 gccgggatag gtgactgtat tgggcgagcc gccgttgaga gtggggaagc cggcggccag
     1681 ctggttgccg ttgtaggcaa agctgacggc gttgaccgcg ccccaggggt agttggcgtc
     1741 gccgccgttg ttggccgaga cggcctccga cgggccgatt tgcacgtcga aaccggtggc
     1801 cagcggggcg aagttgcgga acttgaccac gcccttgatc tgggccgtgg ggattgtgcc
     1861 gggggtgact attggcccca cggcggccag gcggccgatg ggcgcgagca gcgccaccag
     1921 caacagggcg aaaacgagca aacgagcgac gtgtctagac atggcgcaaa cctcctgatt
     1981 gacgccattg cgcccggcgc cgggtttggg ccgacgccgg gcgatgtagc attaggggca
     2041 gacgcggcgc gacggccgcg ctgccgccaa agcatggtta ccagccctgc ggggcgacct
     2101 tgtcgtagtt gccgccggcg aggctgaggt cctggatgtt gaccttggcg tccttgttga
     2161 tgtccgggct gcccaggccg ccgcagtcgc ttatcgccgc gccggtcaga ccgaagtcac
     2221 cgccaataca gctcaggtcg ccgatctcca ccgcggcgtt gttgttggcg tcgccgccca
     2281 ggagacgcgt gttttggccg gtgatggcgg tgccggacac aaccaggtcc ttcttgttgg
     2341 ttagatagag ctggtgcgcg gcctgcagga caaaggtcgc gccgcccggc ggatagagca
     2401 ccgtcaggga gtacgtgccg tcgacggcgc taaacggggc cgacaccggc gtgccgccgc
     2461 cgcgtggcgt gatggtcacc gtgcccgcgt cgatgggcgc cacccgcccc tgtaggctga
     2521 cgcggccgct gatcgtcgcc gtgccgcaga catccacagc gatcgccgcc ggcggcaggg
     2581 tgacggagat ggccacgccg tcctcgtcgg tgagcttcac gtcgtggatg cccagggcaa
     2641 acacgcccgc tttcttagcc gtcaggttga tggttgccac cgtgcccgaa ccgctgacag
     2701 cgggggccgg attgtggagt gtcagggcga acttgcactg gccgggcgtg gttgtgcagt
     2761 tggccgccca agccgtgccg gggctgctgc ccttggcggg gtcgaacagc gccggagaga
     2821 acgcgcccag ggcatcgacc agcgtctggt tgtaatcgac cacgaactcg tagccatacg
     2881 cgccgggcac gttggcgaag tcgatcttga cctggctggt ggagcagacg ttgttggggg
     2941 tcgccgagac gttcagcgcc gcccccggct cgaagtcgta ggtcacgaag cggtcggcgc
     3001 cgttgttgta gccgacgacc agcttgtcga ggctgacggt catggccgaa gagccggagc
     3061 caactttgaa gccgatagcc ccataggtcg ggtggacgcc cgcctcggga taggcggcca
     3121 tgagctgatt ccagctgccg gccgcgccat tgagcgggta gaggggcgcg ccgacgacgt
     3181 tgttcacgac cgggggttgg gtctggaacc agcaggccgc gtcgcgcagg gccagcgggt
     3241 tcagggtctg ccaggtgttg ttgactagcg tcgcgcagcc gggcagcgac ggcacccaga
     3301 ccatgcgccc ctgccaggcc tgcgaggcgt cggtcaggtc gtagtcaacg ttaaagaaga
     3361 agttcggccg ctcgctgctc gccccatacg tatcgtaggt gagttggaag atctggtcca
     3421 gcggcaggcc caactcgaac gggttgaaga tggccgggcc gttggctggc ccggcggtga
     3481 cgaattgggc gctaccgatg ccggccggcg gcgtggccgg gccggcggcc agggccacgt
     3541 agcccgtcgt gccggtgctg ttctcgtcgc aggtcgacca gtcgcgcgtc cagaggctga
     3601 gtacgccacc ggcgcagacg ttggtcggca tgaccatcgt gccgtcggtc ggggcgacaa
     3661 ccatgacgac gttggggtcg cagccctggg tgccgaggca ggtacgcgaa ttgtcgatgg
     3721 gcagagtctg gccgttggcg acatagacaa accagccgta gcggttcccg ccgacgatgc
     3781 cgggcagcgt gtccgcggcg tcggtgagag tctgagtgaa caggacggtt ccgctgctgt
     3841 tgaccagttt caggtcgacg gccagttggg tgctgctctt gtagaacacg tgccggaggg
     3901 tataccagcc ggcggtggtc acttcaacgt ggttgagggt ctccaggtct tcccgaggcg
     3961 caccaccgga gccattggaa gccgcaacca gcagcttgcc ggtggaggtg tccttggcaa
     4021 caaggaaaat gaagtcacgc agatagccgc cgcttgagtt gctggcggcg acagagtaat
     4081 cgaagccggt gccggcgggc caggcggggt cgagatagac gtcgatctcg gccgtccagg
     4141 cgccgggcca cacggagcta tagctgccaa agcgcgtaaa gggcgcgccc tcgccgggcg
     4201 tgccggtgat gacggcgtgg gctacgccct cggccgagac gatgccgttg gttcccgacg
     4261 gtacgcgggc gatggtgcca tagtagctgc cgttgtccag accctggaac cagccgctgg
     4321 tgttcacttc aaagccctga ttctagttaa ccgcggccga ggccacgctg acggccagga
     4381 tcaagataac aaggatgctg aaaacagcgc cgatacgtag gaagcgcgaa tgacgtaagg
     4441 ctgacatggt ggtgatatcc ttttctgaaa gacaatcgcg ccggacagac ccggcgggca
     4501 caagaaacgc ggacagaagg agcgggacgg ccgccggcgt cgccaatgcg cccgtcggcc
     4561 ggtaactgga atgaaatggc gatcagataa agcggtatgg ccctgagcgc acgacctcct
     4621 gagccgggga ggcagtctgg aacggcggtt gcctcgctgg cgtgtcacgt cggggagggg
     4681 cgcgatcgcc tcctggccca actcaagcga acagttcgac gcgcgagact gctgcgctgg
     4741 tgggcactat gagtgaacga aactcatagc ccacttatgc gacagtctag cgggcgcaca
     4801 gagatgagcc tagtgacatt tgtcatcgaa aaacatgaca tttgtcacac gcgctgcggg
     4861 cagtaagggt ttacccttac ctgttcttgg tcaggaggga gtcctggggt ggggtctgag
     4921 agaggaggac aaacggccgg ccgttggcgc ggggcaggcg attgccgccc cgcgccgtcc
     4981 gcgaccggca gcctataccg ctggcggcca gatatcggcc acgcccgcgg ccgattcgtc
     5041 aatgtcggtg cgcgccagcc cgccggcgtc gctgtcgggc acagcgccag ccccggccac
     5101 ctgagccggc ttgggggcgg gcgcggcttt catctcttcg tccggccgca cggggatgcc
     5161 cggcgtcatt tgcgtgtcgg tgctgaccat cgtcgccgcc gcgtcgggat cgatgaggat
     5221 ttcgatctgt tcctcgtcgg tcggcgtgtc cggctcgtcc ccggtcatca tgccgcgggc
     5281 ctgcccgccg agcgcctcgg cgcgctcgac ggccatcgcc gtcgccccgg ccagccgtcc
     5341 ctcagcctga ttggccagga tggtcgccgc cccggcgatg ttgcccgcct ggagttgctt
     5401 catgaagtcg gtctcgcggg cgaacggctc ggcctgcacg tgcgcgccca gccccttgac
     5461 cacgcccagc agttgatcca cagcgttgtc gctgacgatg gccacagccg cgccgctggc
     5521 agcatccagc gcccggccga tggcctccag acgcgggtcg gggatgcccg tgtcgagctt
     5581 tttggcggcc acgccgccca gcagcgcgcc cagcgccgcc gaactgaaca tgccccgccg
     5641 gcggctgagc aagccgacca gcccgccggc cagcgcgccc gcggcggcgc cctgtttgcc
     5701 gcccaggtcg gctgtctcgc ggatggtcag ttggccgctg gcgtccttac ccacgtaggc
     5761 ggcggcgggc atgttgtcat attcgccgcg gaaggtttgc agcaggcggt tgacggcctg
     5821 ggcggccgtc gcttcgtcgt cgaaggtagc gatgatgagt tggaaaccgg tctgttcgct
     5881 catgtgtctc cttcttatcg gggtcattgg ttgtggtgat gtggccagta tagcacaaag
     5941 cgcggccggc ccgtggcgcg attctccgaa tcgcattcgc actccgtggc gcgattctcc
     6001 gaatcgcatt ccgcgccaga gcagaaacgc gattcgggag aatcgcgcca cgcgattcgg
     6061 gagaatcgcg ccacgggccg atgtccta
//
LOCUS       DAOSMQ010000152         6075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13115_length_6075_cov_3.795515, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000152 DAOSMQ010000000
VERSION     DAOSMQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13115_length_6075_cov_3.795515"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK829_06530"
     CDS             complement(<1..539)
                     /locus_tag="PK829_06530"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOU40907.1"
                     /translation="MEVEGMDYQQMRREIDTTGYTIVRGLLTPDEVDYYIGRMEAVSG
                     ITRSGMTRGGDAWKGTGRGSGGSSWTLPDGVVKSRDFWPVATNERLVQIVRNVVDPNV
                     RFLQHNDIHVGFSAISWHRDSVNREYGVGPDWDESQEPYKLVRCGIYLQTFAESNSRL
                     GFIPGSHRPPTGPVAGSTKR"
     gene            complement(666..1763)
                     /gene="hppD"
                     /locus_tag="PK829_06535"
     CDS             complement(666..1763)
                     /gene="hppD"
                     /locus_tag="PK829_06535"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOU40908.1"
                     /translation="MTDDFLPITAIDHLELYCGNARQAAHFYDHGFGFAPVAYSGLET
                     GNRRAASYVLTQGAIRLVLTTALGPDDAIARHVHLHGDGVAVIALAVPDAAAAYRETT
                     ARGATGVVPPSEAGDGDGALRFAAIRAYGDTQIKFVERAGYGGVFAPGYRAMPEAARP
                     RPTAGLTAIDHIVGNVELGAMNRWVEFFARTMGFSQLVHFDDADISTEYSALMSKVMQ
                     DGSGLVKFPINEPAEGKKKSQIQEYLDYYYGPGVQHIACTTNDIVATVRQLRDNGISF
                     LRVPAAYYEELAARVGDLDAPAAALAELGILVDRDEEGYLLQIFTRPVNDRPTLFFEI
                     IERHGSRGFGKGNFKALFEAIEREQAARGNL"
     gene            2065..3552
                     /locus_tag="PK829_06540"
     CDS             2065..3552
                     /locus_tag="PK829_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40909.1"
                     /translation="MMRRFVWLLLSLVLLLAACGARARGAPTPFPTAAATPSPTAGAP
                     IALTVTELAAAPGLYRDAVVQVTGVFRKQPILVCEAEAFRSPATWALGEEGLLVLAGG
                     YDQQVRGLLPEGLLMTAEGRWQRWEGLVGCGKTAVNREVWYLNVGRILSPSPLTQVTL
                     TPGAPGTAVAEAPTVEGQPTLAPTDDFSFPTPLPEETLTEEATFPVDDFPTPTEELFP
                     TIPLPTLEGAETPVASVTVASETPGTPTTGTPTITGTPATATPTVTGTPPTATPTTPS
                     GPGQIITKGSWDDFYEAFTTSIVAAGTTDRWDQEVFEGETYTIYVIAAPPADIVVSLL
                     REDQTIISRQNTAAPGVPEIIAASNITGEGNYQVHVATANGAAAEYAILAGTDPEFPT
                     SIAGYITPGTPRTGIQLAEASTHYWFFTGNAGDELTIVLDPSGGDVYFDLYDPGLEIV
                     DSADDGLEGESETLELTLEMSGLYAIDLAEINGLAITYNLSVTLQ"
     gene            3611..4231
                     /locus_tag="PK829_06545"
     CDS             3611..4231
                     /locus_tag="PK829_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU40910.1"
                     /translation="MDDLLRLIVLADDPLARAGLAALLANLPGCLVVLQGSATLLADA
                     TDGDLDPADAIVWDAGWDAPREREDRLGHALPVLALAPDEDSAAAAWALGCRGVVARA
                     IDEEQLLAAVRAVSAGLHVFSPALAGALRDEAAGVAPTAIDLTPRESQVLGLLAEGLT
                     NKAIAQRLSISDHTVKFHVNAILSKLNAQSRTDAVVRATRQGLISL"
     gene            4357..5253
                     /locus_tag="PK829_06550"
     CDS             4357..5253
                     /locus_tag="PK829_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU40911.1"
                     /translation="MSEAFQTLSDGLAGVVASAGAAIVRIEARRHTPGSGILWSADGL
                     IVTANHVVRRDDNITIGLSDGQTVAATLAGRDPTTDIAVLRAEGVAGAPLEHAAEAEM
                     SVGHLALALARPGRTVQAALGVVSALGDGWRTAMGGQIDHYLATDVVMYPGFSGGPLL
                     GANGRLLGLNTSGLGGDASLAIPASTLSRVVEALRSHGRVRRGYLGISTQRARLPENV
                     ATEAGQKTGLLIIAVESGSPAEAAGLTLGDTLLSLSGKSITAHEDLLAALSGEVVGQK
                     EVARVLRGGQVQELAVKVGERE"
     gene            complement(5339..>6075)
                     /locus_tag="PK829_06555"
     CDS             complement(5339..>6075)
                     /locus_tag="PK829_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU40912.1"
                     /translation="VLQRVVEAAVYVVGAEEGSLMLLDEERGELYIRASKNLDTKARS
                     MRKRVTDSLAGKVLQTKRAIAIGADSQWKRTHTALLVKSLIYVPLILQNKPIGVLGVT
                     NQVKDSSFDSNDTRALSALGGYATIAINNANMFHGLARERTVLSEVMEQIEDAVIVVD
                     EDFRLLVLNAAARALFTMPEDNVIGRPIMDLIDHVELVRMVAEPAAQGGNGRVEQDLA
                     LHGGFYRARVTLIEGGSRFVTLRRLF"
BASE COUNT         1002 a         2024 c         2035 g         1014 t
ORIGIN      
        1 cgcttcgttg aaccagccac tggccccgtc ggcggccggt ggctgccggg gatgaagccg
       61 aggcgcgaat tgctctcggc aaacgtttgc aggtagatgc cgcagcgcac cagcttgtat
      121 ggctcctgcg actcgtccca gtctggcccg acgccatact cccggttgac gctgtcgcgg
      181 tgccaggaaa tggccgagaa gccgacatgg atgtcgttgt gttgcaagaa gcgcacgttg
      241 ggatcgacaa cgttgcggac gatctgcacc aggcgctcgt tggtggccac cggccagaag
      301 tcgcgcgatt taaccacgcc gtcgggcagc gtccacgacg agccgccgga gccgcggccg
      361 gtgcccttcc aggcatcgcc gccgcgggtc atacccgatc gggtgatgcc cgatactgcc
      421 tccatccggc cgatgtagta atccacctca tccggggtca ggaggccgcg cacgatggtg
      481 tagcccgtcg tgtcgatctc gcgtcgcatc tgctgataat ccatcccttc aacctccatt
      541 gccttcacac agacttacct tgcctcatac cgaggccccc ggcgggcctt ctccccgtca
      601 gagtggctgg aagtgtagcg cgaagagaga gaaatgtcag tgggtatttc ggtggattta
      661 gggcgctaga ggttgccgcg ggcggcttgt tcgcgctcaa tagcctcgaa cagggccttg
      721 aagttgccct tgccgaagcc gcgcgagccg tggcgctcga tgatctcgaa gaagagcgtc
      781 ggccggtcgt tcaccggccg ggtgaaaatc tgcaacaggt agccctcctc gtcgcgatcc
      841 accagaatgc ccaactcggc cagcgccgcg gccggcgcgt cgaggtcgcc cacccgcgcg
      901 gctaactcct cgtagtaggc cgcgggcacg cgcagaaacg agatgccgtt gtcgcgcagt
      961 tgccgcacgg tggcgacgat gtcgttggtc gtacaggcga tgtgctggac accggggccg
     1021 tagtagtagt cgaggtactc ctgaatctgc gacttctttt tgccttcggc cggctcgtta
     1081 atggggaact tgaccaggcc agagccgtct tgcatgacct tcgacatgag cgccgagtac
     1141 tctgtgctga tgtcggcatc gtcgaagtgg acgagctggc tgaagcccat cgtccgggcg
     1201 aagaactcca cccagcggtt catcgctccc agttcaacat tgccgacgat gtggtcgatg
     1261 gccgtcaggc cggcggtggg gcgcggtcga gcggcttccg gcatggcgcg gtagccgggc
     1321 gcaaagacgc caccgtagcc ggcccgctcg acaaacttga tctgggtgtc gccataggcg
     1381 cggatggcgg cgaagcgcag cgcgccatcg ccgtcgccgg cctcgctcgg cgggacaacg
     1441 ccggtcgcgc cgcgggcggt cgtctcgcgg taggccgcgg ctgcatcggg cacggccaga
     1501 gcgatgacgg ccacgccatc gccgtgcaag tgaacgtggc gggcgatggc atcatcgggg
     1561 ccgagggcag tggtcagaac aagacggatg gccccctgcg tcaggacata ggaagcggcg
     1621 cgccggttgc ccgtctccag gccgctatag gccaccgggg cgaagccgaa gccgtggtcg
     1681 tagaagtggg ccgcctgccg ggcattgccg cagtagagtt ccaggtggtc gatggccgta
     1741 atgggcagga agtcatcagt catcagcggt ctcctatggc gggcgcggcc acctgccgca
     1801 agtggatgcc caacagtata ggccgctcgc cggcgcgcaa caattggcca aatgaccggc
     1861 cacagccgga cgcaccgcca gggctgttcc aaagtcgtct agcgatcatt gacctgacag
     1921 gtaggcggcc acgaatggca cgtccaccag agaacgactg gccgcctacc tgtcaggtcg
     1981 atgtgccggc ggtgacttta aaaaagcccc ggacgctcag cggaggctca ttcccttggc
     2041 ggcgcaatcg gctatacttg gcccgtgatg cgtcgctttg tctggttgct cttgtcgctt
     2101 gttctgttgt tggccgcctg tggcgcgcgc gcgcgcggcg cgccgacgcc cttccccacg
     2161 gccgcggcca cgccctcgcc cacggccggc gcgccgattg ccctgaccgt gaccgaactg
     2221 gccgccgcgc ccggtctcta ccgggatgcc gtggtgcaag tcaccggcgt cttccgcaag
     2281 cagccgatcc tggtgtgcga ggccgaggcg ttccgctcgc cggccacgtg ggcgctgggc
     2341 gaggaggggc tgctggtgtt ggccggcggc tatgaccagc aggtgcgcgg gctgttgccg
     2401 gaggggctac tcatgaccgc cgaggggcgc tggcagcgct gggagggact ggtcggctgt
     2461 ggtaagacgg ctgtcaaccg cgaggtgtgg tatctcaacg tcggccgcat cctctcgccc
     2521 agccccctga cgcaggtgac gctcacgccc ggcgcgccgg gcacagccgt ggccgaagcg
     2581 ccgacagtag aaggccagcc gacgctcgcg ccaaccgacg acttctcctt tcccacccct
     2641 ctgccggagg aaaccctgac ggaagaggcc actttcccgg tcgacgactt tcccacgccg
     2701 acagaagagc ttttccccac gatccccctg cccactctgg agggcgcgga aaccccggtc
     2761 gcctcggtca cagtcgccag tgagacgccc ggcacgccca ccacgggaac gccgacgatc
     2821 acggggacgc cggcaacagc gaccccgacc gtcaccggca cgccgccaac ggccacgcca
     2881 acgactccca gcggcccggg gcagattatt accaaaggca gttgggacga tttctacgag
     2941 gctttcacta cctccatcgt ggccgcgggc acaaccgacc gctgggatca ggaggttttt
     3001 gagggcgaga cttacacgat ctatgttatc gccgcgccgc cggccgacat cgttgtctcg
     3061 ctgctccgcg aagaccaaac catcattagc cgccaaaaca cggctgcgcc gggcgtgccg
     3121 gagatcatcg ccgcgtccaa tatcaccggc gaaggcaact atcaggttca cgtagcgacc
     3181 gccaacggcg cggcggcgga gtacgcgatt ctggcgggca ccgatccgga gtttccgacg
     3241 agcatcgccg gctatatcac cccggggacg ccccgcaccg gcattcagtt ggcggaggcc
     3301 agtacgcatt actggttctt cacgggcaat gccggcgacg agctaaccat cgttctagac
     3361 ccctctggcg gtgacgtata ttttgacttg tacgaccctg ggctagagat cgtcgactcg
     3421 gccgacgacg gacttgaagg cgagtcggag acgttggaac tgacattgga gatgtccggc
     3481 ctctacgcca tcgatctggc cgagatcaac ggcctggcca tcacctataa cctgtcggtt
     3541 acgctgcaat agcctttcca cccggacaat ctaatcatcc tcatagacag cggcgctatg
     3601 cttgggccac atggatgatc tcctgcggct cattgttctg gccgatgacc ccctggcgcg
     3661 ggccgggctg gcggcgctgc tggccaacct gcccggctgc ctggtcgtgt tgcagggcag
     3721 cgccacgctg ctggccgacg ccaccgacgg cgacctggac ccggccgatg ccattgtgtg
     3781 ggacgccggc tgggacgcgc cgcgcgagcg cgaggaccgg ctgggccacg ccctgccggt
     3841 gctggccctg gcccccgatg aggacagcgc cgccgcggcc tgggcgctgg gctgtcgcgg
     3901 cgtggtcgcc cgcgcgatcg acgaggagca gttgctggcc gctgtgcggg ctgtcagcgc
     3961 cgggctacac gtcttttcgc cggcgctggc cggcgcgctg cgtgatgagg ccgccggcgt
     4021 cgccccgacg gccatcgacc tgaccccgcg cgaaagccag gtgctcggcc tgctggccga
     4081 ggggctaacc aacaaggcca tcgcccagcg cctgtcgatt agcgaccaca ccgttaagtt
     4141 ccacgtcaac gccattctaa gtaagctgaa cgcccaaagc cgcaccgacg ccgtcgtgcg
     4201 ggctacgcgc cagggcctca tctccctcta ggccccctgc ctatcctgct aggctgactc
     4261 cccaccgttc gggcgattcg cgccgcgccg gagggcgcta ttctatgctc aacggcactt
     4321 agccgtgatg cctgaactta gacaaggaga acagcgatgt ctgaagcatt tcaaactctg
     4381 tcagacggac tggccggagt cgtcgcttcg gccggcgcgg ctatcgtgcg catcgaggcg
     4441 cggcgacaca cgcccggcag cggcatcctc tggtcggccg acggcctcat cgtcactgcc
     4501 aaccacgtcg tccggcgcga cgacaatatc actatcggcc tgagcgacgg gcagacggtc
     4561 gcggcgactc tggccggccg cgacccgaca acggacatag ccgtcctgcg ggccgagggc
     4621 gtggccggcg cgccgctgga gcacgccgcc gaggcggaga tgagcgttgg ccatctggcg
     4681 ctggccctgg cccggccggg gcgcaccgtg caggcggcgc tcggcgtcgt cagtgccctg
     4741 ggcgacggct ggcgcacggc catgggcggg cagatcgacc actatctggc caccgacgtg
     4801 gtcatgtacc ccggcttctc cggcgggccg ctactgggcg cgaacggacg gctgctgggc
     4861 ctgaatacct ccggcctggg tggcgacgcc agcctggcta tccccgccag cacgctcagc
     4921 cgtgtcgtgg aggcgttgcg ctctcatggc cgggtgcggc gcggctatct gggcatcagc
     4981 acccagcgcg ctcgcctgcc ggagaacgtc gccacggagg cgggccagaa gacgggcctg
     5041 ctcatcatcg ccgtagagag cggcagcccg gccgaggccg ccggcctaac gctgggcgac
     5101 acgctgctgt ccctcagcgg caagagcatc accgcccacg aggacttgct ggcggcgctg
     5161 tcgggcgagg tggtcggcca gaaggaagtc gcccgcgtgc tgcgcggcgg ccaggtgcag
     5221 gagttggcgg tcaaggtcgg cgaacgcgag tagacgcatc cccggttgag acaagcagag
     5281 cgcccggccc atgcatgtgg gccgggcgct tttttgtatt tccagtcggg cttcgcggct
     5341 agaacaggcg gcgcagcgtg acgaagcggc tgccgccctc gatgagcgtc acgcgggcgc
     5401 ggtaaaagcc gccgtggagg gccaggtctt gctcgacgcg gccgttgccg ccctgcgccg
     5461 ccggttcggc gaccatgcgc accagttcga cgtggtcgat caggtccata atcggccggc
     5521 cgataacgtt gtcttccggc atggtgaaga gggcgcgcgc ggcggcgttg agcaccagca
     5581 ggcggaagtc ctcatcaacg acgatgacgg cgtcctcgat ttgctccatg acctcgctca
     5641 acacggtgcg ctcgcgggcc aggccgtgga acatattggc gttgttgatg gcgatggtcg
     5701 catagccgcc cagggctgac agcgcccgcg tgtcgttgct gtcgaacgac gagtccttga
     5761 cctggttggt gacgcccagc acgccgatgg gcttgttctg caggatgagg ggcacgtaaa
     5821 tgagcgactt gaccagcagg gccgtgtgcg tgcgcttcca ttgggagtca gcgccgatgg
     5881 cgatggcccg cttggtctgc aagaccttgc cggccaggct gtcggtgacg cgcttgcgca
     5941 tagaacgagc tttcgtatcc aggttcttgg aggcgcggat gtacaactcg ccgcgctcct
     6001 cgtccagcag catcaacgaa ccctcttccg cgccgacgac gtagacggcc gcctcgacga
     6061 cgcgctgcaa gacct
//
LOCUS       DAOSMQ010000153         6049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13204_length_6049_cov_4.894061, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000153 DAOSMQ010000000
VERSION     DAOSMQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13204_length_6049_cov_4.894061"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PK829_06560"
     CDS             complement(<1..474)
                     /locus_tag="PK829_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40913.1"
                     /translation="MTTQVPDLYRTLGVGRDATTEAIEAAYAAWEARLSAGEAIATPE
                     WERLRYAHEVLSNPQRRALYDSLVAETAVAGLDLSVAVSAARLPLLATPQLVYALATL
                     AARETTGERRPLTLGLVVDRSTSMRGERLEKVIAAGELLLDRLGPEDALALVSFSD"
     gene            complement(517..1305)
                     /locus_tag="PK829_06565"
     CDS             complement(517..1305)
                     /locus_tag="PK829_06565"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOU40914.1"
                     /translation="MTTPAPMPRLESGYCCETGSFRDRNEDACLTLTAEFGGHFAMWS
                     LGLYVVADGMGGHDAGHVASDTATRVFADYVLAQLYSPLLQRHPAPREAHVLDLLERG
                     VFAAHEAILKSNGNGGSTLTAALVLGSRLYVAHVGDSRAYLADASGLRLLTEDHSLVR
                     RLQDTGQLTDEDARTYQYRNVLLQALGQESALSADTFALDLSPGSTLLLCSDGLSAVV
                     PAEELAASLAADRAQAAAERLVALALARGSEDNVTAVVVRVGRE"
     gene            complement(1313..3277)
                     /locus_tag="PK829_06570"
     CDS             complement(1313..3277)
                     /locus_tag="PK829_06570"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU40915.1"
                     /translation="MEFGTREMPPRKGSDSKPLGRHDANGPQSGEVLQGRYMILGSLG
                     VGGFSSVYRARDLRFPSVTRLCAVKEMVISTVDPEMRELTIKSFEREAGMLATLNHPA
                     IPDIYDYFTDGNRSYLVLEFVPGQNLQQWLDETDEYLDEQKALDWALQVCDALAYLHS
                     QKPQPVIFRDLKPSNIMLDPYNHIRLIDFGIAKLFEANEDKGTMIGTAGYTPPEQYRG
                     EASPAVDVYGLGATLHHLLTRQDPRQETPFTFSERPIRAANPAISRTFEAIIMRCLAY
                     DPKERFPDAMALRESLLELSKSDTDDADLDQRLGLKENALAFDREDTGAPTASLRAGL
                     SRVKPLWVFRCEDEIRSRPAVGKGIVFVAAYDNNLYALTADKGDFLWKFPTGGGIGGS
                     PVVSEDAVLIGSSDHSLYSLQLRKGRLNWQFRAEGPIYSSPAVRFDHAFFGADDGYVY
                     VVNAMRGQLHWKTNVYSPVRSTPWVSDELVYFGTEGGQVLGLELGAGKPKWQTKAQRA
                     VTSSPKVADDILYVGSVDGTVYAIDASSGWPIWRYQTDGPILATPTLYQGVLFIGSSD
                     GHLYAIDMASGRPVWSYHTNGQIVSAPAVWGNTVYFGSTDGGLYGVSLRRGALQWRFD
                     TSSPIIGSPTIVNGVIYFGAADHTLYALPT"
     gene            complement(3460..4398)
                     /locus_tag="PK829_06575"
     CDS             complement(3460..4398)
                     /locus_tag="PK829_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOU40916.1"
                     /translation="MKRYDHLYLSPHFDDAILSCGGQIARHTAAGQSVLVVTITGGDP
                     PDNPPSDTVAMLHRRWAASMAAVPGPVHSDGRWPASPRGADSASRAQTSAASSAAASS
                     AASSAIAVGAAMIAQRRAEDRAALALVGAEPLHLSFLDCIYRCGPDAHALYPGPADMF
                     SPLSPADEATVAALAESFAALPPARQVYLPLGVGGHVDHLATRRAAERVFTAPIYYED
                     YPYTARPGALAEALPPEVRAVWLATAVWLDEAALAAKTAAVGAYVSQLSSFFVDAADL
                     AAQLRADGRRALSDALAGGETAPPWAVAAERLWSAA"
     gene            4469..4948
                     /gene="moaC"
                     /locus_tag="PK829_06580"
     CDS             4469..4948
                     /gene="moaC"
                     /locus_tag="PK829_06580"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOU40917.1"
                     /translation="MSDRLTHLDESGRARMVDVGAKDVTERVAVARGRVTMRPATLAL
                     IVAGELPKGDVLTVAQIAGVMAAKRTSELIPLCHPLLLNQVVVTCAPNAAEQCIDIEA
                     TVRVSGKTGVEMEALTAVTVAGLTLYDMAKAVDRGMRLTDVRLVRKSGGRSGTWEDA"
     gene            5111..5869
                     /locus_tag="PK829_06585"
     CDS             5111..5869
                     /locus_tag="PK829_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOU40918.1"
                     /translation="MTVELEIRLFATLKDRAGRDRIRVELPDRPATAADVLTAVAASY
                     PALAPALPTSLLAVNRAFAGPDTPVKTGDEVALFPPVSGGQSDAPAVEASALATYFAV
                     SPDPLDIDAVHARLGGPDIGALVSFTGFVRGRTQRDGLPAETLHLEYEAYEAMATEKM
                     AQIAQEIRARWPMVRGVALVQRLGRLDVGEVTTFVACAGRHRDQGVFEAARYGIDRLK
                     EIVPVWKKEVGADRSVWLEGHYRPTPADNLKPDS"
     gene            5914..>6049
                     /locus_tag="PK829_06590"
     CDS             5914..>6049
                     /locus_tag="PK829_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOU40919.1"
                     /translation="MSNNENGARRRVVVTGVGLITPLGHNVPDTWAAILAGRSGFGPI
                     T"
BASE COUNT          972 a         1948 c         2188 g          941 t
ORIGIN      
        1 gtcgctgaag ctgaccaacg ccagggcgtc ttccggcccc agccggtcca ggagtaactc
       61 gcccgcggcg atgaccttct ccagccgttc gccgcgcatg gaggtactgc gatcgacgac
      121 aaggccaagc gtcagcggcc ggcgctcgcc ggtcgtctcg cgcgcggcca gcgtcgccag
      181 ggcatagacc agctgcggcg tggccagcag gggcagccgc gcggcgctga cggccacgga
      241 caggtcaagg ccggccacgg ccgtctcggc caccagcgag tcatagaggg cgcggcgctg
      301 cgggttgctg agcacctcgt gggcatagcg caggcgctcc cactccgggg tggcgatggc
      361 ctcgccggcg gacaagcgcg cttcccaggc ggcataggcc gcctcgatcg cctcggtcgt
      421 cgcgtcgcgg ccgacgccga gggtgcgata gaggtcgggt acttgcgtgg tcatgagtcg
      481 gggaggggtg gggcggcgcc gccggctgtc ggcgtactac tcccggccga cgcgcaccac
      541 gaccgcggta acgttgtcct cgctgccgcg agccagggcc agggcgacga gccgttcggc
      601 cgcggcctgg gcgcggtcgg cggccagcga ggcggccagc tcctcggcgg gcacgacggc
      661 gctcagcccg tcgctgcaca gcagcagcgt actgcccggc gacaggtcga gggcgaaggt
      721 gtcggccgaa agggcgctct cctggcccag cgcctgcaat aggacattgc gatactggta
      781 agtacgggcg tcctcgtcgg ttagctggcc agtgtcttgc aggcggcgga cgagggaatg
      841 atcctcggtc agcaggcgca ggccgctggc atcggccagg taggcgcgcg aatcgccgac
      901 gtgggccaca tagaggcgcg agccgagtac cagcgctgcc gtcagcgtcg agccgccgtt
      961 gccgttgctc ttcaaaatgg cctcgtgggc ggcgaaaacg ccgcgctcta gcagatcgag
     1021 cacgtgggcc tcgcggggcg cggggtggcg ttgcagcaga ggagaataga gctgggccag
     1081 aacgtagtcg gcgaagacgc gcgtggcggt gtcgctggcc acgtgcccgg cgtcgtgccc
     1141 gcccatgccg tcggcgacga cgtacaaccc cagcgaccac atggcgaagt ggccgccgaa
     1201 ctcggccgtc agcgtcagac aggcgtcctc gttgcggtcg cggaaggagc cagtttcaca
     1261 gcagtagccg ctttcaaggc ggggcatggg cgcgggcgtg gtcatgagcg gctcaggtgg
     1321 gtagggcgta gagggtgtgg tcggcggcac cgaagtagat gacgccgttg acgatggtcg
     1381 gcgagccaat gatggggctg ctggtatcga agcgccattg gagcgcgccc cggcgcaggc
     1441 tgactccata gaggccgccg tcggtcgagc cgaagtagac ggtattaccc cacacggccg
     1501 gcgcggagac gatctggcca ttggtgtgat agctccacac cggccggccg ctggccatgt
     1561 cgatggcgta gaggtggcca tcggacgagc cgatgaacag cacgccctgg tagagcgtcg
     1621 gcgtggccag gattggccca tcggtctgat agcgccagat gggccagccg gagctggcgt
     1681 cgatggcgta gacagtgcca tcgaccgagc caacatacag gatgtcgtcg gcgaccttgg
     1741 gcgaggaggt caccgcgcgc tgagccttgg tctgccattt aggtttgccc gcgcccagtt
     1801 ccaggcccaa cacctggccg ccctctgtgc cgaagtagac caactcatca gacacccacg
     1861 gtgtcgagcg caccgggcta tagacgttgg ttttccagtg gagttgcccg cgcatggcgt
     1921 tgacgacgta gacgtagcca tcatcggcgc caaagaaggc gtggtcgaag cgcacggccg
     1981 gcgaggagta gattggcccc tcggcccgga actgccagtt gagccgcccc ttacgcagtt
     2041 gcagcgagta gagcgagtgg tcggacgagc cgatgagcac ggcgtcctcg gacacgactg
     2101 gcgagccgcc gatgccgccg ccggtgggga acttccacag gaagtcgccc ttgtcggctg
     2161 tcagggcata gaggttgttg tcgtaggcgg ccacgaagac gatgcccttg cccacggccg
     2221 ggcgcgagcg aatctcgtcc tcgcaacgga agacccacag cggcttaacc cggctcagac
     2281 cggcgcgcag cgacgccgtg ggcgcgccag tgtcctcgcg gtcgaaggcc agggcattct
     2341 ctttcagacc gaggcgctga tcgaggtcgg cgtcgtcggt gtcgcttttg gacagttcca
     2401 gcagtgactc gcgcaaggcc atggcatccg gaaagcgctc cttggggtca taggccaggc
     2461 agcgcatgat gatggcctcg aaggtgcgcg aaatagccgg gttggcggcg cggatggggc
     2521 gctcgctgaa ggtgaagggg gtctcctggc gcgggtcctg gcgggtgagc aggtggtgga
     2581 gggtggcccc caggccatag acatcgacgg ccggggaggc ttcgccgcgg tactgctccg
     2641 gcggcgtgta gccggccgtg ccgatcatcg tgcccttgtc ctcattggcc tcgaacagct
     2701 tggcgatgcc gaagtcgatg aggcggatgt ggttgtaggg gtcgagcatg atgtttgacg
     2761 gcttcaggtc gcggaagatg accggctgcg gcttctggct gtgcaaatag gccagagcgt
     2821 cacagacttg cagcgcccag tcgagtgcct tctgctcatc gaggtactca tcggtctcgt
     2881 cgagccactg ctgcaagttc tggccgggca caaattccag caccaggtag ctgcggttgc
     2941 cgtcggtgaa gtagtcgtag atgtcgggaa tggccgggtg gttgagcgtg gccagcatgc
     3001 ccgcctcgcg ctcgaaggat ttgatcgtca gttcgcgcat ctccgggtca accgtgctga
     3061 tgaccatctc cttgacagcg cacaggcgcg tgaccgaggg aaagcgcagg tcgcgcgccc
     3121 gatagacaga gctgaagccg ccgacgccca gggagccgag gatcatatag cgcccctgga
     3181 gcacctcgcc cgactggggg ccgttggcgt cgtggcggcc gaggggtttg ctgtcgctgc
     3241 cttttcgggg cggcatttct cgcgtgccga attccatagt cgtgatccta accggcgttg
     3301 tctaccggta cagtagcgtt ggtggcgtca ctcgccgacg cgctatcatt atagggccgc
     3361 ggggggtgtt ttcccaaatg aatttagcgt gaacgcggga cgagtggtca gacggcgcgg
     3421 cgcgccttcg tcgtcgcccg cgccgaaaag cgcccaacac taggccgccg accagaggcg
     3481 ctcggcggcg acggcccacg gcggcgcggt ctcgccgccg gccagagcgt cgctcagggc
     3541 gcggcggccg tcggcgcgca gttgggcggc caggtcagcg gcgtcaacga agaaggaact
     3601 gagttgcgaa acgtaggccc ccaccgccgc cgtcttggcc gccagcgcgg cctcgtcgag
     3661 ccagacggcc gtggccagcc agacagcgcg aacctccggc ggcagcgcct cggccagcgc
     3721 gcccggccgc gcggtatagg gatagtcctc gtagtagatg ggcgcggtga agactcgctc
     3781 ggcggcgcgg cgcgtggcca ggtgatcgac gtggccgccg acgcccaacg gcaggtagac
     3841 ctggcgggca ggcggcaggg cggcgaagct ctccgccagg gcagccaccg tggcctcgtc
     3901 agccgggctg aggggactga acatgtccgc cgggccgggg tagagggcat gcgcgtcggg
     3961 gccgcagcgg tagatgcagt cgaggaagga caggtgcagc ggctcggccc ccaccagcgc
     4021 cagcgcggcg cggtcttcgg cacggcgttg ggcgatcatg gctgcgccca cggctatggc
     4081 gcttgaggcg gcgctcgagg cggcggcgct cgaggcggcg ctcgtttgcg cgcgcgacgc
     4141 gctatctgcc ccccgcgggc ttgccggcca gcgcccatcg ctgtggacag ggccaggcac
     4201 ggcggccata ctggcggccc agcgccggtg gagcatggct accgtgtccg agggggggtt
     4261 gtcgggcggg tcgccgccgg tgatggtgac gacgagaacg gactggcccg cggctgtgtg
     4321 ccgggcgatc tgcccgccgc acgacagaat ggcgtcgtcg aagtggggtg agaggtaaag
     4381 gtggtcgtat cgcttcattg agggactcgc ttagaaaccg agttcccagg aagaaactcg
     4441 gtttctgggc tggtataata tcataatcat gagcgaccgc ctgacccacc tggacgaatc
     4501 tggccgcgcc cgcatggttg atgtcggcgc gaaagatgtg accgagcgcg tggccgtggc
     4561 ccgaggccgc gtcacaatgc ggccggcgac gctggcgctg atcgtcgccg gcgagctgcc
     4621 caagggggac gtgctcaccg tggcccagat cgccggggtc atggccgcca agcgcaccag
     4681 cgaactgatc cccctctgtc acccgctgct gctcaaccag gtcgttgtca cctgcgcgcc
     4741 caacgcggcc gagcagtgca ttgacattga agcgacggtg cgcgtgtcgg gcaagacggg
     4801 cgtggaaatg gaagccttga cggccgtcac tgtcgccggg ctgacgctct acgatatggc
     4861 caaggccgtc gaccgcggca tgcgcctgac cgacgtccgt ctcgtgcgta aatcgggcgg
     4921 gcgcagcggc acatgggagg acgcataggg gcagacctgt gggtctgccg cgcccgttgc
     4981 gttgcgtgtc tgcccgcctg cgtgccggcc gcactttccc atgcatctgc catactatct
     5041 gcccgctgtc ccaccggcag cgtgcacacg tctggcgcat cgaccacggc gctcaagggt
     5101 tacaaataag atgacagtcg aactggaaat ccgccttttc gccacgctga aggaccgcgc
     5161 cgggcgcgac cgcattcgcg tggaactgcc cgaccggccg gcgacggcgg ccgatgtgct
     5221 caccgccgtg gcggccagct atccggcgct ggccccggcc ctgcccacgt cgctgctggc
     5281 cgtcaaccgc gcctttgccg ggccagacac gcccgtgaaa accggcgatg aggtggccct
     5341 cttcccgccc gtcagcggcg gccagagcga tgcgccggct gttgaggcga gcgccctggc
     5401 gacctacttc gccgtctcgc ccgacccgct ggacatcgac gccgtccacg cccgcctcgg
     5461 cggcccggac atcggcgcgc tcgtcagctt caccggcttt gtccgcggcc gcacgcagcg
     5521 cgacggcctg cccgcggaga cgctgcacct ggaatatgag gcctacgagg ccatggccac
     5581 ggagaagatg gcccagatcg cccaggagat tcgtgcccgc tggccaatgg tgcgcggcgt
     5641 ggccctggtg cagcgactcg gccgcctgga cgtgggcgag gtgacgacat ttgttgcctg
     5701 cgccggccgc caccgcgatc agggcgtctt tgaggcggcc cgctatggca tcgaccgtct
     5761 caaggagatc gtgccggtgt ggaagaagga ggtcggcgct gaccgcagcg tctggctgga
     5821 gggccactac cgcccaaccc cagccgacaa cctgaagcct gattcttgac cccaggccag
     5881 tctagcgtcc tggcattaac ttctggagca tctatgagca acaacgagaa cggagcgcgc
     5941 cgccgcgtcg tcgtcaccgg cgtcgggctg atcacgccac tgggccacaa cgtgcccgac
     6001 acctgggcgg ccatcctggc cgggcggtcg ggcttcggcc ccatcaccc
//
LOCUS       DAOSMQ010000154         6011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13328_length_6011_cov_4.759402, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000154 DAOSMQ010000000
VERSION     DAOSMQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13328_length_6011_cov_4.759402"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PK829_06595"
     CDS             <1..635
                     /locus_tag="PK829_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40920.1"
                     /translation="DEPPVSIATTTAAGTATAAAATATTAAATATALALAGNPTSTAT
                     PTPLPATAGLPTPTLQPTPALLPSPTLFPSPVPPPTATVEPLPAPFTALLLIDPEDDS
                     VIRALRADDIVDLREIDRNHLTVLAEVDTAVVESVSFFLDGAPFCPRGNCVENTAPFY
                     MGGDQGGNAYDDWDWSEMLGSHTLSAIACTGDNATGACFPAVAVNLVIRR"
     gene            646..2799
                     /locus_tag="PK829_06600"
     CDS             646..2799
                     /locus_tag="PK829_06600"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOU40921.1"
                     /translation="MPPPPRSARRRDARRRAAALVLLVLGLVMVGAAVWLWGRTPAVS
                     TQLAVSTQSAASTQSAASTQSAASTQSAASTQSAASTQSAVSTQSAASTPAPSPAATA
                     APTSGLSFSPALLSGYDSNAPLNCAPTAGPGTVTLDGTLAVWQPLRLRFTGPAATEAG
                     SDPNPFLDYRLTVRFVGPSGRAYSVPGFFAGDGQGGGAGDQWAARFAADEPGRWRWCA
                     SFRAGPGVAVALDPAAGRSLAFDGAAGTFEVAPAEPNAPGFFALGRLEYAGGHYLKFR
                     HGPYWLKTGANSPENFLAYSGFEDTADTDANAFLHSYAAHIADWRPGDPTLPGAADEG
                     KGIIGALNYLAGAGVNSIYFLPMNLGGDGNDTWPFLSPEDVTHYDVGKLAQWRVVFEH
                     AQRRGIGLHIVLNETESANRRWFDGGAVVDDILSPPRQLFYRELVARFADLPAIKWNL
                     SEESVFSRDEAIALAGYLGALDWADHPIAVHNPADWFGPLEDILGREEFSLTSIQYQI
                     DEAGALVEAWRANSARAGRPWVVELDENRPAQEGLTPDNAGALRKTVLYDALFSGAGG
                     VEWYLGDHALPVGGDLNVEDFRTRADMWAYSLYARRFLEDNAPFWLMAPADELLSGEA
                     ADYGGGEVLARPGEVYAVYLPSAALPASLAAPDGGYTLRWYNPRSGVFAGEPLALTAA
                     GGALPLGSPPADSAADWVALITAAAYTPPPPPAYP"
     gene            2974..3195
                     /locus_tag="PK829_06605"
     CDS             2974..3195
                     /locus_tag="PK829_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40922.1"
                     /translation="MADNRIIVEFDGERYTQISGAWYNAQTYIKPPEIISRRLDERFG
                     VARPPEKPSRPPRRPPPSARPARPARPPL"
     gene            3416..4678
                     /locus_tag="PK829_06610"
     CDS             3416..4678
                     /locus_tag="PK829_06610"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU40923.1"
                     /translation="MKTITGPLLAVPDAVRVSERAEREQLHQDLLVTSSTIMALLSLL
                     WGLIYLYIGAVTAGAIPIVYAALSFASVGSYALTGRYHFFRFTQLLLTLVFPAMLMLV
                     LGGFINSSGVILWSLTSPVGAMLFAGRRQATGWFAAFAVFVLVAGLIDGGVLPTPFPV
                     ATLPTMDLTTTFFIMNIIGPSVVVFLLLAFSTRQRDRSRELLLLEQAKSEQLLLNVLP
                     QSIAARLKAGEGRIAECYDDASILFADIVGFTPLSAELGVAVVVDLLNDIHSAFDAIV
                     ARHGLEKIRTIGDGYMVVAGVPTPRPDHAHALVDAALEMLAFVRDWPSPYAPRIRHRI
                     GINSGAIMAGVIGRAKFSYDVWGDPVNVASRMESSGLPDCIQVSERTHALIKDDFICH
                     PRGIMDIKGKGQMPTWLVEGRRKVVNGI"
     gene            complement(4766..5191)
                     /locus_tag="PK829_06615"
     CDS             complement(4766..5191)
                     /locus_tag="PK829_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOU40924.1"
                     /translation="MSYSDQEVIQRILRTAKHIAVVGLTDDPTRPSYFVPAYLQANGY
                     HIIPINPTLSGSVLGETVYASLADAPGPIDVVQIFRRSEEVEPIVEQAIAAGARAVWM
                     QLGISNEEAAAVAHAAGLDVVMNQCMKVQHSRLSGETLI"
     gene            5230..>6011
                     /locus_tag="PK829_06620"
     CDS             5230..>6011
                     /locus_tag="PK829_06620"
                     /EC_number="2.5.1.115"
                     /inference="COORDINATES: protein motif:HMM:NF009525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate phytyltransferase"
                     /protein_id="HOU40925.1"
                     /translation="MTGNQAAVAPLRPAALARFSRLHTVVATSVQVAGLYALAGGAAA
                     APATALLLTWAASLAANLYVVGLNQLTDVAIDRLNKPALPLASGELSAGQARAIVLAA
                     GGAALLLAATQGAALALTLAAVMFIGTAYSLPPTRFKERALPAALSIALARGVVANVG
                     VYSNFAGGLRGAPPSFLVALPFFFGFGLVIALFKDIPDWAGDGRYGVGSFAVRWGVRR
                     AFAVGRALLTALYLLPIGALLAWPGPAAATLIAARVALLAVF"
BASE COUNT          849 a         2257 c         1914 g          991 t
ORIGIN      
        1 ccgatgagcc gcccgtcagt attgccacca caacggccgc cggcacggcc acggccgccg
       61 ccgcgacggc cacaaccgcc gccgcgacgg ccacggcact ggctctggcc ggcaacccca
      121 cgtctaccgc cacgcccacg cccctgcccg ccaccgccgg cctcccgacg cccacactcc
      181 agcccacgcc ggcgctgctg ccctcgccca cgctctttcc gtcgcccgta ccgccgccga
      241 cggccacggt cgagccgctg cccgcgccct tcacggccct gctgctgatc gatccggagg
      301 acgacagcgt catccgcgcc ctgcgggccg acgacatcgt cgatttgcgc gagatcgacc
      361 gcaaccactt gaccgttttg gcagaggtcg ataccgcggt cgtcgagagc gttagcttct
      421 tcctcgacgg cgcgcccttc tgcccgcgcg gcaactgcgt cgagaatacc gcgcccttct
      481 acatgggcgg cgaccagggc ggcaacgcct acgacgactg ggactggtca gagatgttgg
      541 gcagccacac gctgagcgcc atcgcctgca ccggcgacaa cgccacgggc gcatgcttcc
      601 cggcggtggc ggtcaacctg gtcattcgcc ggtagcctgc cgctgatgcc tccaccgccc
      661 cgctccgccc gccgtcgcga cgcccgccgt cgcgccgcag cgctcgtgct gctggtgctc
      721 gggctggtca tggtgggcgc ggccgtctgg ctgtggggcc gcacgccggc tgtgtccacg
      781 cagttggctg tgtccacgca gtccgctgcg tccacgcagt ccgctgcgtc cacgcagtcg
      841 gctgcgtcta cgcagtcggc tgcgtctacg cagtccgctg cgtccacgca gtcggctgtg
      901 tccacgcagt ccgctgcgtc cacgcccgcg ccctccccgg ccgcgacggc cgcgcccacc
      961 agcggcctgt ccttctcccc ggcgctgctc tccgggtacg acagcaacgc cccgctcaac
     1021 tgcgcgccca cggccggccc gggcacggtg acgctcgacg gcacgttagc cgtctggcag
     1081 ccgctccggc tgcgctttac cggcccggcg gcgaccgagg ccggcagcga ccccaacccc
     1141 ttcctcgact accgcctgac cgtccgcttc gtcggcccgt ccggccgggc ctacagcgtg
     1201 cccggcttct tcgccggcga cggccagggc ggcggcgcgg gcgaccaatg ggcggcgcgc
     1261 ttcgccgccg acgagccggg gcgctggcgc tggtgcgctt cctttcgcgc cgggccgggc
     1321 gtggccgtgg ccctcgaccc ggccgcgggc aggtcgctgg ccttcgacgg cgcggcgggc
     1381 acgttcgagg tcgccccggc cgaacccaac gcgccgggct tcttcgccct cggccggctg
     1441 gagtacgccg gcggccacta cctcaagttc cgccacggcc cttactggct aaagacgggc
     1501 gcgaacagcc ctgagaactt cctagcctac agcggcttcg aggacacggc cgacaccgac
     1561 gccaatgcct tcctgcacag ctacgccgcc cacatcgccg actggcggcc aggcgaccca
     1621 acgctgcccg gcgcggccga cgagggcaag ggcatcatcg gcgccctcaa ctacctggcc
     1681 ggcgccggcg ttaactccat ctacttcctg cccatgaacc tcggcggcga cggcaacgac
     1741 acctggccct tcctctcgcc cgaagacgtg acccactacg acgtcggcaa gctggcccag
     1801 tggcgggttg tctttgagca tgcccagcgc cgcggcatcg gcctgcacat cgtgctgaac
     1861 gagaccgagt cggccaaccg gcgctggttc gacggcggcg cggtcgtgga cgacatcctc
     1921 agcccaccgc gccagctttt ctaccgcgag ttggtggccc gctttgccga tttgccggcc
     1981 atcaagtgga acctgagcga ggaaagtgtc ttcagccgcg acgaggccat cgccctggcc
     2041 ggctatctgg gcgcgctcga ttgggccgac catcccatcg ccgtccacaa cccggcggac
     2101 tggttcgggc cgctggagga catcctcggc cgcgaggagt tctccctgac ttccatccag
     2161 taccagatcg acgaagccgg agcgctggtg gaggcgtggc gggccaactc ggcgcgggcg
     2221 gggcggccgt gggtggtcga attggacgag aaccgcccgg cgcaggaggg cctgacgccc
     2281 gacaacgccg gggcgctacg caaaaccgtc ctctacgacg cccttttcag tggcgcgggc
     2341 ggcgtggagt ggtatttggg cgaccatgcc ctgcccgtcg gcggcgatct gaacgtcgaa
     2401 gacttccgca cgcgggcgga catgtgggcc tattcgctct acgcccgccg ctttctggag
     2461 gacaacgcgc ccttctggct gatggccccg gccgacgagt tgctgagcgg cgaggcggcc
     2521 gattatggcg gcggcgaggt gctggcccgg ccgggcgagg tttacgcggt ctacctgccc
     2581 tcggccgctc tgccggcaag cctggccgcg cccgacggcg gctacaccct gcgctggtat
     2641 aacccacgca gcggtgtttt cgccggcgag ccgctggccc tgaccgctgc cggcggcgcg
     2701 ctgcccctcg gctcgccgcc ggccgactcg gccgccgatt gggtcgccct catcactgcc
     2761 gccgcctaca ccccgccccc gccccccgcc tacccgtagg gacgcacccg catgtgcgct
     2821 ccctgacccc tatcccccaa tccccaatcc ctaatcccca ttgttatacg cattgtaatg
     2881 tctccgccct aaaatgagag acaggtaagg ataaaagggg atagtggcgg cgcggcgacc
     2941 ggggaatccc gcgccccagg gacacagcaa gagatggccg acaatcgcat catcgtcgag
     3001 tttgacggcg aacgctacac gcagatcagc ggcgcgtggt acaacgccca gacctacatt
     3061 aagccaccgg agatcatcag ccgccgcctg gacgagcgct tcggcgtcgc ccggccgccg
     3121 gagaaaccca gccgcccgcc gcgccgcccg ccgccatccg cgcgcccggc gcgaccggcg
     3181 cgcccgccgc tgtaaccggg gcatcgcccg gtctacagaa gcgcacgcac agctcacgcc
     3241 aaggcgctaa ggggcgaaag gcgccaagaa gaaagagccg atgacagagt tccttcttgg
     3301 cggccttggc ttactttgcg actttgcgtg agcgcttctc ccgccctgat aatgcccgta
     3361 gccgctgtag cctgggctac tgcccattag cggcgcatcg gctatactgg agggcgtgaa
     3421 gaccatcacc ggccccctgc tggccgtgcc cgatgccgtg cgcgtgtcgg agcgcgccga
     3481 gcgcgaacag ctccaccagg acctgctcgt cacctcctcg acgatcatgg ccctgttgag
     3541 cttgttgtgg ggcctgatct acctctatat cggcgccgtc accgccgggg ctatccccat
     3601 cgtctacgcg gccctgtcct tcgccagcgt cggctcctat gccctgaccg gccgctacca
     3661 ctttttccgc ttcacccagc tgctgctcac gctggtcttc ccggccatgc tcatgctcgt
     3721 cctcggcggc ttcatcaact ccagcggcgt catcctctgg tcactgacct cgcccgtcgg
     3781 ggcaatgctc ttcgccgggc ggcgccaggc cacgggctgg ttcgccgcgt tcgccgtctt
     3841 tgtcctcgtc gccgggctga tcgacggcgg ggtgctgccg acacccttcc ccgtggccac
     3901 gttgccgacg atggacctga cgacgacatt tttcatcatg aatatcatcg gcccgtcagt
     3961 tgtcgtcttc ctgctgctgg ccttttccac gcgccagcgc gaccgctcgc gcgagttact
     4021 gctgctagag caggccaaat cagagcaatt gctgctcaac gtcctgccgc aatcgatcgc
     4081 cgcgcggctg aaggcgggcg aggggcgcat cgccgaatgt tacgacgacg ccagcatcct
     4141 ttttgccgac atcgttggct tcacgccact gtcggccgag ttgggcgtgg cggttgtcgt
     4201 cgatctgctc aacgacatcc acagcgcctt cgatgccatc gttgcccgcc acggtctgga
     4261 gaaaatccgc accatcggcg acggctacat ggtcgttgcc ggcgtgccca cgccccgccc
     4321 cgaccacgcc cacgccctgg tcgatgccgc cctggagatg ctcgccttcg tgcgcgactg
     4381 gccgtcgccc tatgcaccac gcatccgcca ccgcattggc atcaacagcg gcgcgatcat
     4441 ggccggcgtc atcggccggg ccaagttcag ctacgacgtc tggggcgacc cggtcaacgt
     4501 cgccagccgc atggaatcat cgggcttgcc cgactgcatc caggtcagcg agcgcaccca
     4561 cgcgctgatc aaggacgact tcatctgcca cccgcgtgga attatggaca tcaagggcaa
     4621 gggccagatg ccgacctggt tggtggaggg gagaaggaag gtagtgaatg gaatttgagc
     4681 tattcccggc gctctttgtg ctgacaccca tcacgctggg catcgccctc ctgctggtca
     4741 tctggcaccg gcgcggccgc gagttctaga tcagcgtctc gccgctcagt cgcgagtgct
     4801 gcaccttcat gcactggttc atgaccacgt ccagcccggc ggcgtgggcg acggccgcgg
     4861 cttcctcgtt gctgataccc aattgcatcc acaccgccct ggctcccgcg gcgatggcct
     4921 gctcgacaat cggctccacc tcctcgctgc ggcggaagat ctgcaccacg tcaatcggcc
     4981 ccggcgcgtc ggccagcgag gcatagaccg tctcgcccag cacgctgccg ctgagcgtgg
     5041 ggttgatggg gatgatatga tagccgttgg cctgaagata ggcgggcaca aagtagctcg
     5101 gccgggtcgg gtcgtcggtc agtccgacaa cagcgatatg cttggccgtg cgcagaatac
     5161 gttggatcac ttcctggtcg gagtaagaca tgggcgcatt ataccgtagt tgccgcgcgg
     5221 gaaacacgca tgaccggtaa ccaggccgcg gtcgcgcccc tacgcccggc cgccctggcg
     5281 cgcttcagcc ggctgcacac ggtcgtggcc acaagcgtgc aggtggccgg actctatgcc
     5341 ctggcgggcg gcgcggccgc ggccccggcg acggcgctcc tgctcacctg ggcggccagc
     5401 ctggcggcca acctttatgt cgtcgggctg aaccagctta ccgacgtggc catcgaccgg
     5461 ctcaacaagc ccgctctgcc gttggccagc ggcgagctgt cggccgggca ggcgcgggcc
     5521 atcgtcctgg ccgccggggg cgcggccctg ctgctggcgg cgacgcaggg cgcggccctg
     5581 gccctgacgc tggcggcggt gatgttcatc ggcacggcct actcgctgcc gccgacgcgc
     5641 tttaaggagc gagcgctgcc ggcggctctc agcatcgccc tggcccgcgg cgtcgtggcc
     5701 aacgtcggcg tctacagcaa cttcgccggc ggcctgcgcg gcgcgccgcc gagcttcctg
     5761 gtcgccctgc cgttcttctt cggctttggc ctggtcatcg ccctgttcaa ggacatcccc
     5821 gactgggccg gcgatgggcg ctatggcgtc ggctcgttcg ccgtgcgctg gggcgtgcgg
     5881 cgcgcgtttg ccgtcggccg cgccctgctg acggcgctct acctgctgcc catcggcgcg
     5941 ctgctggcct ggcccggccc ggccgccgcg acgctcatcg ccgcccgcgt cgccctgctg
     6001 gccgtcttct g
//
LOCUS       DAOSMQ010000155         6008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13337_length_6008_cov_5.402486, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000155 DAOSMQ010000000
VERSION     DAOSMQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13337_length_6008_cov_5.402486"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK829_06625"
     CDS             <1..489
                     /locus_tag="PK829_06625"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422796.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU40926.1"
                     /translation="IAAEPTASPSLTKGRYTFDYGDTAQMAPIVKMYTLGHTFIPPGI
                     HAGGLRYHGMAPQVSALLHGGQIEARAVKQLDTFKAGLTFLRAEGIIPAPESTHAIAV
                     AIDEALRCKAAGEKKVIAFNLSGHGHFDMTAYDSYLKGQLEDYEYPVEAVEEALQHLP
                     AV"
     gene            565..2100
                     /gene="trpE"
                     /locus_tag="PK829_06630"
     CDS             565..2100
                     /gene="trpE"
                     /locus_tag="PK829_06630"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOU40927.1"
                     /translation="MVTFDSTAETLRPTLDEFRALARGAANLIPVTREFAADLETPVS
                     VYLKLMEEPGASFLLESVEGGEQVGRYSFVGVGLHRRVELRGQTVAWSGLPTDDLPSG
                     DAPGDILDAVRDVLARYRPAPVPGLPRLSGGAVGYMGYDVVRFFERLPETAAPGLDLP
                     DAIFLLVDTLVVFDHARHRLVLLANAHVPDGGDVEAAYVDALRRIDRLAEKLLRPLPA
                     VPIRRWGATHGNGQALEANMTRAKHEAMVLRAKEHIAAGDIFQVVLSQRFSRHTSAHP
                     FAVYRALRMLNPSPYMFYFNFAPLDLQVIGASPEMHVRLEDGVATVRPIAGTRRRGAN
                     HAEDEALAAELLADPKERAEHVMLVDLGRNDIGRVCQYGTVRVHDLMTIERYSHVMHI
                     VSHVEGRVRPGLDAYDLMRATFPAGTVSGAPKVRAMEIIEELEGQRRGLYAGAVGYFS
                     YDGSMDTCIAIRTMVMQGDRVYVQAGGGIVADSDPAAEYQETVNKAGALLVAVARAEA
                     GAL"
     gene            2157..3182
                     /gene="trpS"
                     /locus_tag="PK829_06635"
     CDS             2157..3182
                     /gene="trpS"
                     /locus_tag="PK829_06635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU40928.1"
                     /translation="MSDKKRVFSGIQPTGNTHLGNYLGAVRNWAARQAEKVNTFCVVD
                     LHSLTVPQDPEQLRHETRSMAALLLACGIDPAQSTLFVQSHVAAHAEGCWLLNCVTPL
                     GWLQRMTQFKDKSARQESVLTGLLDYPVLMAGDILFYDADEVPVGEDQKQHVELTRDI
                     AQRFNALYEQEFFVIPQPVIPEVGARVMGLDDPTVKMSKSLADKRGHAVRMLDTPDEI
                     LYSFKRAVTDSGREIAFSNAPEKAGVNNLLGIYRAITGKSNAEVEADFAHARGYGDLK
                     VRVAEVVIETLRPIQARHRALMDDTAELDRILAHGAEQARAIAQPKVDAMKQIMGLVL
                     PGKVAAG"
     gene            3279..4274
                     /locus_tag="PK829_06640"
     CDS             3279..4274
                     /locus_tag="PK829_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40929.1"
                     /translation="MKRLALFLLLALAPAAMTACGRTPAEATATELPPPPTAATTVAM
                     PTVATDAATTTAPALATTAPATATAEPSAPASPEASPTTAPAATDTAAAASPTPAATA
                     AATATAMATAPASTPATGSIFGPGQVDGSTLAAGSAHSYLVQGRPFEPIILFIEPENE
                     LDVALAAYSGDLSGQTTPEGVTALAAADNALAGRPEILVLSPESAGLYTFVIRAASGE
                     GNFVAHLFDLTTPAPGVAIQQADALEAGTEKVFAVASNGARPVIVVVDPSDQSDIALD
                     IIGADGALLTTANYSGPGGAETGYVLPLGATSLSVKVREVNGAASAFQIVVITLQ"
     gene            4359..4919
                     /locus_tag="PK829_06645"
     CDS             4359..4919
                     /locus_tag="PK829_06645"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOU40930.1"
                     /translation="MVLVIDNYDSFTYNLVQYLGELGAELRVYRNDEITLDEIAALRP
                     THIVISPGPGTPDDGGISNEVIRRFGPTTPVLGVCLGHQCMGQVYGGVVARAPRLMHG
                     KTSSVYHNGQGLFNGVPSPFTATRYHSLIVEEPLPEALVVTAFTRDGEVMGLQHREYP
                     VAGVQFHPESILTEHGKRILQNFLAM"
     gene            4982..5251
                     /locus_tag="PK829_06650"
     CDS             4982..5251
                     /locus_tag="PK829_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOU40931.1"
                     /translation="MRFTWHEDKRRQTLAKRGLDFADAEKIFNGPTFTFEDDRRDYGE
                     RRWVTLGLLREKVVVIVHTETEDEIRVISMREAEANEQLLFFTNL"
     gene            5220..5519
                     /locus_tag="PK829_06655"
     CDS             5220..5519
                     /locus_tag="PK829_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOU40932.1"
                     /translation="MSNSFSSPISDDDPDDYPTITQADLDRATFRVGRQPAPRKQRVT
                     IMLDTRLIEYFKAQAGERGYQTLINETLRRAMEAEDLEARLRRIVREEVRAARGS"
     gene            5557..>6008
                     /locus_tag="PK829_06660"
     CDS             5557..>6008
                     /locus_tag="PK829_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU40933.1"
                     /translation="MTVIYGFELLREEMVPELNTRARLWRHVRTGAELLSLENDDENK
                     VFGITFRTPPADSTGLAHILEHVVLAGSEKYPLKDPFFEMVKGSLATFINAMTYPDKT
                     VYPAASTNLQDFYNLLDVYADAVFHPLMTPEKLAQEGWHYELDSADGPL"
BASE COUNT         1083 a         2050 c         1940 g          935 t
ORIGIN      
        1 atcgccgccg agccgacggc ctcgcccagc ctgaccaagg ggcgctacac cttcgactac
       61 ggcgacacgg cgcagatggc ccccatcgtc aagatgtaca ccctcggcca caccttcatc
      121 ccgccgggca tccacgccgg cggcctgcgc taccacggca tggccccgca ggtcagcgcc
      181 ctgctccacg gcggccagat cgaggcgcgg gccgtcaagc aactcgacac cttcaaggcc
      241 ggtctgacct ttctgcgcgc cgagggcatc atccccgcgc ccgaatcgac ccacgccatc
      301 gccgtggcca tcgacgaggc gctgcgctgc aaggccgccg gcgagaagaa ggtcatcgcc
      361 tttaacctca gcggccacgg ccacttcgac atgaccgcct acgacagcta cctgaagggg
      421 cagctggagg actacgagta cccggtcgaa gcggtggaag aggcgttgca gcatttgccg
      481 gcagtgtagt ggcgcgattc tccgaatcgc tctctgaggg tatagatagc gcgattcggg
      541 agaatcgcgc cacggatgga gactatggtc acatttgatt caacagccga gaccttacgg
      601 ccgaccctgg acgagttccg cgccctggcg cggggggcgg ccaatctaat cccggtcacg
      661 cgcgagttcg ccgccgacct ggaaacgccc gtctccgtct acctgaagct gatggaggag
      721 ccgggggcgt cgttcctgct ggagtcggtc gaagggggcg agcaggtcgg ccgctattcc
      781 ttcgtcggcg tcggcctgca tcgccgggtg gaactgcgcg ggcagacggt ggcgtggagc
      841 ggcctgccca ccgacgacct gcccagcggc gacgcgccgg gcgacatcct cgacgctgtg
      901 cgcgatgtgt tggcccgtta ccggcccgcg cccgtgcccg gcctgccgcg gctcagcggc
      961 ggcgcggtcg gctacatggg ctacgacgtg gtgcgcttct tcgagcgcct gccggagacg
     1021 gccgcgcccg gccttgacct gcccgacgcc attttcctgc tggtggacac actggtcgtc
     1081 ttcgaccacg cccgccaccg gttggtgttg ctggccaacg cccacgtgcc cgatggcggc
     1141 gacgtggagg ccgcctacgt ggacgcgctg cggcgcatcg accggctggc ggagaagctg
     1201 ctgcggccgc tgccggccgt gcccatccgc cgctggggcg cgactcacgg caacggccag
     1261 gcgctggaag ccaacatgac ccgcgccaag cacgaagcca tggtgttgcg ggccaaagag
     1321 cacatcgccg ccggcgacat cttccaggtc gtcctcagcc agcgcttcag ccgccacacc
     1381 agcgcccacc ccttcgccgt ctaccgcgcc ctgcggatgc tgaacccgtc gccctacatg
     1441 ttttacttca acttcgcgcc gctcgacttg caggtcattg gcgcgtcgcc ggaaatgcac
     1501 gtgcgattgg aggacggcgt ggccacggtc cggcccatcg ccggcacgcg ccgccgcggg
     1561 gccaaccacg ccgaggacga ggcgctggcc gccgaactgc tggccgaccc caaagagcgg
     1621 gccgaacacg tcatgctggt tgacctgggg cgcaacgaca tcggccgggt gtgccagtat
     1681 ggtacggtgc gcgtccacga cctgatgacc atcgagcgct atagccacgt gatgcacatc
     1741 gtcagtcacg tcgaggggcg ggtgcggccg ggcctggacg cctatgacct gatgcgggcc
     1801 accttcccgg ccggaacggt cagcggcgcg cccaaggtgc gggcgatgga gatcatcgag
     1861 gaactggagg ggcaacggcg cggcctctac gccggcgcgg ttggctactt cagctatgac
     1921 ggtagcatgg acacctgcat cgccattcgc acgatggtca tgcagggcga ccgcgtctac
     1981 gtccaggccg gcggcggcat cgtcgccgac agcgacccgg cggccgagta ccaggagacg
     2041 gtcaacaagg ccggcgcgct gctggtcgcc gtggccaggg cggaagcggg agcgttatag
     2101 aatgaggaat gaggaatgga gaaagccttc ttgattccca attcatcaga ggtgttatga
     2161 gcgataaaaa acgagtgttt agcggcattc agccgacggg gaatacccac ctgggcaact
     2221 acctgggcgc ggtgcgcaat tgggcggcgc ggcaggcgga gaaggtcaac actttttgcg
     2281 tcgttgacct ccacagcctg accgtgccgc aagacccgga gcaactccgc cacgagacgc
     2341 gctcgatggc ggccctgctg ctggcctgcg gcatcgaccc ggcccagagc acgctgttcg
     2401 tccagagcca cgtcgccgcc cacgccgagg gctgctggct gctcaactgc gtcacgccac
     2461 tgggctggct gcaacgcatg acccagttca aggacaagtc cgcccggcag gagagcgtcc
     2521 tgaccggcct gctcgactac ccggtgctca tggccggcga catcctcttc tacgacgccg
     2581 acgaggtgcc cgtgggcgaa gaccagaagc agcacgtcga actgacgcgc gacatcgccc
     2641 aacgcttcaa cgcgctctat gagcaagagt tcttcgtcat cccgcagccg gtcattccgg
     2701 aagtgggcgc gcgggtcatg ggcctggacg accccacggt gaagatgtcc aagagcctgg
     2761 ccgacaagcg cggccacgcc gtgcgcatgc tcgacacgcc cgacgaaatc ctctacagct
     2821 tcaagcgggc cgtgaccgac tccggtcgcg agatcgcctt ctccaacgcc ccggagaagg
     2881 ccggcgtcaa caacctgctg ggcatctacc gggccattac cggcaagagc aacgccgagg
     2941 tggaagccga cttcgcccat gcccgcggct atggcgacct gaaggtgcgc gtggccgagg
     3001 tggtcatcga gacgctgcgg cctatccaag cgcgtcaccg cgcgctgatg gacgacacgg
     3061 ccgagttgga ccgcatcctg gcccacggcg cggagcaggc ccgtgccatt gcccagccga
     3121 aggtagacgc gatgaagcaa atcatgggtc tggtacttcc gggcaaggtg gcggccggat
     3181 gagtgggaca ttcgtctcat ggggcggcgg cgtgtgtgga ctacaatagg gcgtcggccg
     3241 ttgccaatgg ggcgcggcca cgattcatag ggggttcaat gaaacgactc gctctattcc
     3301 tgctgctggc tttagcaccg gcagcgatga ccgcctgtgg ccggacgccg gccgaggcga
     3361 cggccacgga actgccgccg ccgccgacgg ccgcgacgac cgtcgctatg cccacggtgg
     3421 ccaccgacgc ggcgacgact accgcgccgg ccctggcgac caccgcgccg gccacggcaa
     3481 cggccgagcc gtcagcacca gcctcgcccg aagcgtcgcc gacgacggct ccggccgcca
     3541 ccgacaccgc cgcggcggcc tcgcccaccc ctgcggcgac agccgcggcc acggccacag
     3601 cgatggccac cgcgccggcg tccacgcccg ccactggcag catcttcggc cccggccagg
     3661 tggacggctc gacgctggcg gccggcagcg cccacagcta tctggtgcag gggcggccgt
     3721 tcgagccgat catccttttc atcgagccgg agaacgaact ggacgtggct ctggcggcct
     3781 acagcggcga cctgagcggc cagacgacgc ccgaaggggt gacggcgctg gccgcggccg
     3841 acaatgccct ggccgggcgg ccggaaattc tggtgctcag cccggaaagc gccggcctgt
     3901 acacgttcgt catccgcgcc gcctcaggcg agggcaactt cgtcgcccac ctctttgacc
     3961 tgaccacgcc cgcgcccggc gtggccatcc agcaggccga cgcgctggaa gccggcacgg
     4021 aaaaagtctt tgccgtggcc tcgaacgggg cgcggccggt catcgtcgtg gtcgacccgt
     4081 ccgaccaatc cgacatcgcc ctggacatca tcggcgcgga cggggcgctg ctgacgacgg
     4141 ccaactacag cggccccggc ggcgcggaga cgggctacgt cttgccgttg ggggctacgt
     4201 cgctgtcggt caaggtgcgc gaggtcaatg gggcggcgtc ggcgttccag attgtggtca
     4261 ttacgctgca atagtggcgc gattctccga atcgcggtct tgtactagtg gacataattg
     4321 cgccacgcgg gagaatcgcg ccacgcggga ggacaatgat ggttctggta atcgacaact
     4381 acgattcatt cacctacaac ctggtgcaat acctgggtga gttgggggcg gagttgcgcg
     4441 tctatcgcaa cgatgagatc accctcgacg agatcgccgc gctgcggccg acccacatcg
     4501 tcatcagccc cggcccgggc acgcccgacg acggcggcat ctccaatgag gtcatccgcc
     4561 gctttgggcc gacgacgccg gtgctgggcg tctgcctggg gcatcagtgc atgggccagg
     4621 tctatggcgg cgtcgtcgcg cgcgcgccgc ggctgatgca tggcaagacc tccagcgtct
     4681 accacaacgg ccagggcctc ttcaacggcg tgcccagccc cttcacggcc acgcgctacc
     4741 actcgctgat cgtcgaggag ccgctgccgg aggcgctggt cgtcaccgcc ttcacgcgcg
     4801 acggcgaggt gatgggcctg caacaccgcg agtaccccgt cgccggcgtc cagttccacc
     4861 cggagagcat cctgaccgag cacggcaagc gcatcttgca gaacttcctg gcaatgtaat
     4921 tgtctactca cctacccaca tcttgcttga ctaatgcata taatcatgca tataataccc
     4981 gatgcgcttc acgtggcatg aggacaagcg tcgacagacg ctagcgaagc gcggcctgga
     5041 ttttgccgac gcggaaaaaa tcttcaatgg gccgacattt acgttcgaag atgatcgccg
     5101 cgattatggc gagcggcgct gggttacact gggtcttttg cgcgaaaaag tagtggtcat
     5161 cgtccacacg gagacggagg acgaaatccg ggtcatttct atgcgcgagg ctgaagccaa
     5221 tgagcaactc cttttcttca ccaatctctg acgacgatcc cgacgactac ccgacgatta
     5281 cccaggccga tctcgaccgg gcgacattcc gtgtcggtcg ccagcctgcc ccacgcaagc
     5341 aacgcgtgac catcatgctc gacacgaggc tgatcgagta tttcaaggcc caggccggcg
     5401 aacgcggcta ccagacgctc atcaacgaaa cgctgcgacg ggcgatggag gccgaggatc
     5461 tggaagctcg tctgcgccgc atcgtgcgcg aggaagtgcg ggcggcccgt gggtcataag
     5521 ccggcgacca ctagcccctc aacccaggag ttaatcatga ccgtcatcta cggcttcgaa
     5581 ctacttcgcg aggagatggt tcccgaactg aacacgcgcg cccgcctgtg gcggcacgtg
     5641 cgcaccggcg cggaactgct gtcgctggag aacgacgacg agaacaaagt cttcggcatc
     5701 accttccgca cgccgccggc cgactcgact ggcctggccc acatcctgga gcacgtcgtc
     5761 ctggccggct cggagaagta cccgctcaaa gaccccttct tcgagatggt caaaggctcg
     5821 ctggcgacgt tcatcaacgc catgacctac cccgacaaga cggtctatcc ggcggccagc
     5881 accaacctgc aagacttcta caatctgctc gatgtctacg ccgacgccgt cttccacccg
     5941 ctgatgacgc cggagaagct ggcccaggaa ggctggcact acgagttgga cagcgctgat
     6001 ggccccct
//
LOCUS       DAOSMQ010000156         6007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13343_length_6007_cov_4.324933, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000156 DAOSMQ010000000
VERSION     DAOSMQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13343_length_6007_cov_4.324933"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PK829_06665"
     CDS             complement(<1..1047)
                     /locus_tag="PK829_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOU40934.1"
                     /translation="MTTPTDIPVPLRRSGEVDASPAAATRLHFLDHLRAVIILLVILL
                     HASMTYMAYPPEWWYVIEPRNSLALTGLVLLLDVPNMQILFFIAGFFAYGSLEKYGPA
                     RFLRQKALRLGLPWLVGVVFLAPLVTYLIPYSRGIAPPYLQFWAVDFWGVYYQQSVYW
                     FLGMLLLLFALLAAFYHGEPSLQAIPRRAQMPDSKFLAKFWAFMTLWYVVAGAVVPPD
                     TWLNALKVIVYQPARLGLYVGYFVLGLIADRRGWFRAGGYQPDLEFWGPAAFMSAAGY
                     VGLRLSGIEGGVALLAVEAMLFNALCLSALMAGVALFQTRVNRPTRVWASLDRNAYAI
                     YYLHPLVLYPATYLA"
     gene            complement(1129..2199)
                     /locus_tag="PK829_06670"
     CDS             complement(1129..2199)
                     /locus_tag="PK829_06670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HOU40935.1"
                     /translation="MDPINLYEYEALAQARLTSMAYDYYASGAHDEITLRENHAAYDR
                     IALRYRVLRDISQRSTATTVLGHNLSMPVLVAPTAFHKLAHPEGEVATVRAAGHAGTL
                     MILSTLSTTSIEDVLAAATGPVWFQLYVYKDRGATKALVERAEAAGCAALALTVDAQV
                     WGRRERDVRNRFQLPAGLSVKNLMPAGQEAFPKEAQDSGLAAYVASLFDQTLSWKDVE
                     WLCGLTKLPVLLKGVVHPEDARQAADYGAAGVIVSNHGGRQLDTAPATIAALPDIVDA
                     VAGRVEVLIDGGIRRGTDVVKALAYGARAVAVGRPVLWGLAADGQRGVERVLDILRFE
                     VDLAMGLCGCASVAEIGRDLLG"
     gene            2698..3486
                     /locus_tag="PK829_06675"
     CDS             2698..3486
                     /locus_tag="PK829_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40936.1"
                     /translation="MVPKKVTNVLIGGLVALGLLGGVATATFAQTATPSAPSTTVPDA
                     TTPSTSISDDDQALADQLGITLAELQAAEEEARIAIIDQAVADGKITAEEGAALKAAS
                     SGLHGSRFGYDKNVFLAAALGISLDELQAAKLAVYKAEVADRVTAGELTQAQADLLVA
                     QKAAGYYLDTDALNATVRSAYETAIAAAVAAGDITQAQADALLAQLPTTTFNFGFGGG
                     HGGHGGGHGHGGGRGFGDSLVVPDTTTPDSTTPDATTTDTSFGA"
     gene            complement(3598..5421)
                     /locus_tag="PK829_06680"
     CDS             complement(3598..5421)
                     /locus_tag="PK829_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40937.1"
                     /translation="MVDAIEKPSAAITPARSRYPHLLPALFFLLLVLVANFPLATHLN
                     SHVVSSAADDTFEVLWQLVATERAVFKTHTNPFFTPDVFYPHGWYLASGAQPTWYYTL
                     LSPLTAAVGPVATYNLISLGTFVAAGFGVYLLGQWLTGRRLAGVIAGCAYILAPFFTA
                     RVGGHTHVLFAMMFLPYALGATLRAMVAPARAWRWVVLGGVALAATILSHWYFLFIAT
                     LPVVAAALSVHSTVAWRARLARLAVLGGIALALISPALLLTQQARAAMFPQGGTFRLS
                     DIELHGISPDYYLGPNPLNPWLRAWAGRVFPVHGEADIAALGYTTLALAIAGLLATPR
                     RQTRPFVVMGLLAFVLSLGATLRWRGERVLVAAPPWLVRLVSPLTRDLVLPAGQLPVL
                     LPNVLLYYALPFYSSLRVWARYAAPLQLAMALLAGFGAAWLLGRGRWGAVLAVVLGGL
                     LVLEGWTLPYITFNDVAINDRTVNDWLAARPEGTAIIEYPRPWVDTTAMYSQALHGQS
                     VVNGYMSFMPDYLAHVDGQLGEWPSKAAIPLYREWGIDYVVVSRMDNDAAFRETIWPE
                     ISAIGALCPVADFPDAFRFHDFTATAVFAVRPFPGAPCPTP"
     gene            complement(5442..>6007)
                     /locus_tag="PK829_06685"
     CDS             complement(5442..>6007)
                     /locus_tag="PK829_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40938.1"
                     /translation="ALLAGYGAAWLVRRGRAGQVAAAALGALIIFEGVIVPYRDFTPV
                     SANDRAVNAWLAAQPQGTAVIEYPLPWVNTLAMYSQSLHGQQVVNGFMSFMPTFLTEA
                     SAQLGEWPNENALPLLREWGVDYVLVSGLPQVEVYRDTILPSIETLDGLCLVQRFPDA
                     LAMMDFRETLVFAVVEPGVACPPLSAR"
BASE COUNT         1155 a         1998 c         2038 g          816 t
ORIGIN      
        1 agccaggtaa gtcgccgggt agaggacgag gggatggaga tagtagatgg cataggcgtt
       61 gcggtcgagg ctggcccaga cgcgcgtggg gcgattcacc cgcgtctgga agagcgccac
      121 cccggccatg agcgccgaca gacacagggc attgaagagc atggcctcga cggccaggag
      181 ggcgacgccg ccctctatcc cgctgaggcg caggcccacg tacccggcgg ccgacatgaa
      241 cgccgccggc ccccagaact ccaggtctgg ctggtagccg ccggcgcgaa accagccccg
      301 ccggtcggcg atgaggccca gaacgaagta accgacgtag aggcccaggc gcgccggctg
      361 gtagacgatc accttcaggg cattcagcca ggtatccggc ggcaccaccg cgccggcgac
      421 gacgtaccag agcgtcatga aggcccagaa cttggccagg aacttactat ccggcatctg
      481 cgcccggcgc gggatcgctt gcaggctcgg ctcgccgtgg tagaacgcgg ccagaagcgc
      541 aaacaggagc aacagcatcc ccaggaacca gtagaccgac tgctggtaat agacgcccca
      601 gaagtcgacc gcccaaaatt ggaggtaggg cggcgcgatg ccacgcgagt acgggatcag
      661 ataggtgacc agcggcgcga gaaagacgac gccgaccagc cagggcaaac ccaggcgcag
      721 cgccttctgg cgcagaaacc gggccgggcc gtatttctca agcgacccgt aggcaaagaa
      781 cccggcgatg aagaacagga tttgcatgtt gggcacgtcc agcagcagca ccagccccgt
      841 cagcgccagg ctattccgcg gctcgatgac ataccaccac tccggcgggt aagccatgta
      901 ggtcatgctg gcgtgcaaca ggatgaccag caggatgatg accgcgcgca ggtggtcgag
      961 aaaatggaga cgggtcgcag cggccggcga ggcatccacc tcaccggatc ggcgcaatgg
     1021 aaccgggatg tcggttggcg tggtcatagg gcaaacccct gttcagttcc gggccgcggg
     1081 gccgggcggc gcggggcggc ccgcgcaaca aacgcagggc gtcaaggctc agcccagtaa
     1141 gtcccggccg atctccgcca cgctggcgca gccacacaac cccatcgcca ggtcaacctc
     1201 gaagcgcagg atgtccagca cgcgctcgac gccgcgctgg ccgtcggcgg ccagccccca
     1261 caacaccggc cggccaacag ccaccgcccg cgcgccatag gccagggcct taaccacgtc
     1321 cgtgccccgg cggataccgc catcgatcag cacctcgacg cgcccggcca cggcatcgac
     1381 gatgtcgggc agcgcggcga tcgtcgccgg ggccgtgtcc agttgccggc cgccgtggtt
     1441 gctgacgatg acgccggcgg caccatagtc ggccgcctgc cgcgcgtcct ccgggtggac
     1501 gacgcctttc aacagcaccg gcaacttcgt caggccacac agccactcga cgtccttcca
     1561 cgacagcgtc tggtcgaaca gcgacgcgac gtaggccgcc agcccggagt cctgggcctc
     1621 cttgggaaac gcctcctgcc cggcgggcat caggttcttg accgacagcc cggccggcag
     1681 ctggaagcgg ttgcgcacgt cgcgctcgcg gcggccccag acctgcgcgt ccacggtcag
     1741 ggccagcgcc gcgcagccgg ccgcctcggc ccgctccacc agcgccttcg tcgccccgcg
     1801 gtctttatag acgtagagtt ggaaccacac cggcccggtg gccgcggcca gcacgtcctc
     1861 gatagaggtc gtcgagaggg tgctgaggat catcagcgtg ccagcgtggc ccgcggcgcg
     1921 gacggtggcc acttcgccct cggggtgggc cagcttgtgg aaggcggtcg gggcgacgag
     1981 cacgggcatg ctcaggttgt ggccgaggac ggtcgtagcc gtgctgcgtt ggctgatgtc
     2041 gcgcaggacg cgatagcgca gggcgatacg gtcgtaggcg gcgtggttct cgcgcagggt
     2101 gatctcgtca tgagcgccgc tggcgtagta gtcgtaggcc atcgacgtca atcgggcctg
     2161 ggcgagggct tcatactcgt agaggttgat ggggtccatg atgtttgctc tcaggggcaa
     2221 gtgggcgtgg caacgatgtg cggaatgaca tcacgcggca gacgcttcag ttcttcagcc
     2281 gctgacgact tccattcaat tcggtagaag gccaatggat agcgtctaga ttgaccatag
     2341 tgtctctcgt ctcccaatat ccattataac atgccgtggc gcgattctcc cgaatcgcgc
     2401 cctccgcgat tctggagaat cgcgccacct cattgccaac accatcgacg ccggcaccca
     2461 cgtgaccgcg ttcaacggca gcccggacag catcgtcacc aatggcttca ccctgtacgt
     2521 caatggctgg cggcgaacgg agccggcgca actcatctgc cactaggcaa ccgccaacag
     2581 gttcccaaca acatccttaa attgtctgaa catttggcgg ctatcctgtg gacagtgaaa
     2641 caaaaaacca gcgccctccc cgcgagcggc gcaaacagaa caaggagatc gtgtaagatg
     2701 gttcccaaga aagtgacgaa cgttttgatc ggtggtttgg tggccctggg gctgctcggc
     2761 ggcgtcgcca cggcgacgtt tgcccagaca gcgaccccga gcgcgccgtc caccaccgtg
     2821 ccggacgcga ccacgcccag cacgagcatc agcgacgatg accaggccct ggccgaccag
     2881 ctgggtatca ccctggcaga gctacaggcg gccgaggaag aggcccggat cgccatcatc
     2941 gaccaggcgg tagccgacgg caagatcacc gccgaagaag gcgcggcctt gaaagccgcg
     3001 agcagcggcc tgcacggcag tcgcttcggc tacgacaaga acgtgttcct ggccgcggct
     3061 ctgggcatca gcctggatga gctgcaagcg gccaagctgg ccgtctacaa ggccgaagtg
     3121 gccgaccgtg tgaccgctgg cgagttgacc caggctcagg ccgatctgct ggtggcccaa
     3181 aaggcggccg gctactatct cgacaccgac gcgctgaacg ccacggtgcg gtcggcctat
     3241 gagacggcca ttgccgcggc ggtagccgcc ggtgacatca ctcaggccca agccgacgcg
     3301 ctactggccc agctgccgac gacgaccttt aacttcggct ttggcggcgg ccacggcggc
     3361 cacggcggcg gccacggcca cggcggcgga cgcggcttcg gcgattcgtt ggtcgtcccg
     3421 gatacaacga ccccggatag caccaccccg gacgcgacga cgacagacac gagcttcggc
     3481 gcttaagcca ggccactccc ttgcttccgg tgggccaccg gtagccccga tggcccaccg
     3541 tttctgaccc tccttacgca aagagcgcgt cgccccaagg acgcgctttt tgtttgccta
     3601 tggggtcggg cagggcgcgc cggggaaggg gcgcacggcg aagacggccg tggcggtgaa
     3661 gtcgtggaag cggaaagcgt cggggaagtc cgccacaggg cagagcgcgc cgatggcgct
     3721 gatttccggc cagatcgtct cgcgaaaagc ggcgtcgttg tccatgcggc tgacgacaac
     3781 gtagtcgatg ccccactcgc ggtaaagggg gatggccgcc ttgctgggcc actcgcccag
     3841 ttggccgtcc acgtgagcca ggtagtcagg catgaaggac atgtagccgt tgaccacgct
     3901 ctggccgtga agcgcctggc tatacatggc cgtcgtgtcc acccacggcc gcgggtactc
     3961 gatgatggcc gtgccttccg gccgcgccgc caaccagtcg ttgaccgtgc ggtcgttgat
     4021 ggccacgtcg ttgaaggtaa tatagggtag cgtccagccc tccaagacaa gcagcccgcc
     4081 tagcacgacg gccagcacgg ccccccatcg cccccgcccc agcaaccagg ccgcgccgaa
     4141 gccggccagc agggccatgg ccagttgcag cggcgcggcg tagcgcgccc agacgcgcag
     4201 agaggagtag aacggcaggg cgtagtagag caggacgttg ggcagcagga caggcaactg
     4261 cccggccggc aacaccaggt cgcgcgtcag cggcgagacg aggcggacaa gccacggcgg
     4321 cgcggccaca agcacgcgct cgccgcgcca gcgcagtgtc gcccccaggc tgagcacaaa
     4381 ggcgagcagg cccatgacca caaacggccg cgtttgccgc cggggcgtgg ccagcagacc
     4441 ggcgatggcc agcgccagcg tggtatagcc cagcgcggcg atgtcggcct cgccgtgcac
     4501 ggggaaaacg cggcccgccc aggcccgcag ccacgggttc agcgggttgg ggccgaggta
     4561 gtagtcgggg ctgatgccgt ggagttcgat gtcggacaga cggaacgtgc ccccctgtgg
     4621 gaacatggcc gcgcgcgcct gctgggtcag cagcagcgcc ggggagatga gcgccagggc
     4681 gatgccgccc aatacggcca gccgcgccaa ccgcgcccgc caggccaccg tcgagtggac
     4741 gctcagcgcc gcggcgacga ccggcagggt ggcgatgaac aggaagtacc agtgcgacag
     4801 gatcgtcgcc gccagggcga cgccgcccag gaccacccag cgccacgccc gcgccggcgc
     4861 gaccatagcc cgcaacgtcg cccccagggc gtagggcaga aacatcatgg cgaagagcac
     4921 gtgggtgtgg ccaccgacgc gggcggtgaa gaacggggcc aggatgtagg cgcagccggc
     4981 gatgaccccg gccagccgcc ggcccgtcag ccactgcccc agcagataga cgccgaagcc
     5041 agcggcgacg aacgtaccca gggagataag gttgtaggtc gccaccggcc cgaccgcggc
     5101 cgtcagcggc gacagcagcg tgtagtacca cgtcggctgt gccccgctgg ccagatacca
     5161 gccgtggggg tagaagacgt cgggcgtgaa gaacggattg gtgtgcgtct tgaagacggc
     5221 ccgctccgtg gccaccagtt gccagagcac ctcgaaggta tcgtcggcgg cgctgctaac
     5281 cacgtggcta ttcaggtgcg tcgccagcgg gaagttggcc acgagcacca gtagcaggaa
     5341 gaacagcgcc ggcaggaggt ggggatagcg cgaacgcgcc ggcgtgatgg ccgcggacgg
     5401 cttctcgata gcgtcaacca tggccatctc agggcaatga atcagcgggc tgagagcggc
     5461 gggcaggcga cgcccggttc caccacggcg aagacgaggg tttcgcgaaa gtccatcatg
     5521 gccagagcgt cgggaaagcg ctgcaccagg cacaggccat caagggtctc aatgctgggc
     5581 aggatggtat cgcggtacac ctccacttgc ggcaagccgc tcacgagcac gtaatcgacg
     5641 ccccattcgc gcaagagggg cagcgcgttc tcgttgggcc actcgcccag ttgcgcgctt
     5701 gcctcggtca ggaacgtcgg catgaaggac atgaagccgt tgacaacttg ttggccgtgc
     5761 aacgactggc tatacatggc cagggtgttc acccacggca gcggatattc aatgacggcc
     5821 gtgccctgtg gctgcgcggc caaccaggcg ttgacggcac gatcgttggc cgacacgggc
     5881 gtgaagtccc ggtagggcac aatgacgccc tcgaagatga tgagcgcgcc caatgccgcc
     5941 gcggccacct gcccggcccg gccgcggcgc accagccagg ccgcgccata gccggccagc
     6001 aacgcca
//
LOCUS       DAOSMQ010000157         5995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13370_length_5995_cov_5.372054, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000157 DAOSMQ010000000
VERSION     DAOSMQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13370_length_5995_cov_5.372054"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK829_06690"
     CDS             complement(<1..206)
                     /locus_tag="PK829_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40939.1"
                     /translation="MYVTKGWAKVTILAALLAALLLGTFMFAPAAHAKGGDATRIALK
                     GSAQYPNVKGTAKYKAAGGEREFQV"
     gene            392..1828
                     /locus_tag="PK829_06695"
     CDS             392..1828
                     /locus_tag="PK829_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOU40940.1"
                     /translation="MMRSTALSRLSLTQQFMIASLIILVAAALGLGRWAVRQIEQSVV
                     SRTAETTALFVDSFIAPNLQELSTSDTLTPDHIATLDRLLDDTPLGRQILSFKVWDAT
                     GKVLYSAEPATIGQVFPVGEELTRAWSGQVSSQISDLKDVENALERTQRAHLLETYAP
                     VRLENSDEIIAVAEFYQTVDELERDISLAQGRTWLVVGASMLVIYLLLAGFVGRAGDT
                     IDRQQAALSRQVKQLTDLLDQNTALHQRVQRAAARTTALNERVLRRISAELHDGPVQD
                     IGLALLRLDNVVAQVDDGKTAGDGKTGGDAAPAIVHDLDMVQASMQRALLEIRALSSG
                     MGVPQLGALTLPETLARAVRVHQQRSGTEVVLDLDGTPERASLPVKITIYRLVQEALH
                     NAYRHAGGIDQRVTLCYDAGRLHIEVADGGAGFGQADSAAGDDHLGLAGMRERVESLG
                     GNFRVESAPGQGTRVIADLSLAGEEEVT"
     gene            1825..2484
                     /locus_tag="PK829_06700"
     CDS             1825..2484
                     /locus_tag="PK829_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU40941.1"
                     /translation="MSETIRIVLIDDHPLFREGVAITLAAQPDIEIIGQGENVGDAVR
                     LARELAPDLMLLDVNIPGGGGAQAAQQVAAANSAIGIIMLTASDEEDDLTAAMKAGAR
                     GYVLKGVAARELVGIVRSVHAGEGYVMPALAARLLSDMTAARRPSRHANPLDALTERE
                     RQILDCVAAGLSNREVGDQLHLSEKTVKHYMTIIMEKLQVRNRVEAALLARNATGHDA
                     N"
     gene            complement(2493..2777)
                     /locus_tag="PK829_06705"
     CDS             complement(2493..2777)
                     /locus_tag="PK829_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOU40942.1"
                     /translation="MPPGVERSESAALARTDEDGDVTALGSAVRGLLDCLPGEYRQAL
                     ILTDYHGLSPGDIAGRLDIAPAAAEARIRRGRAMLREALLDWGQFLSRPF"
     gene            complement(2997..3203)
                     /locus_tag="PK829_06710"
                     /pseudo
     CDS             complement(2997..3203)
                     /locus_tag="PK829_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
     gene            complement(3209..>5995)
                     /locus_tag="PK829_06715"
     CDS             complement(3209..>5995)
                     /locus_tag="PK829_06715"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU40943.1"
                     /translation="VDLPRGRFLFAAGWWLAAVVWAWLRLGPGHRHWLRLGPAFVAAA
                     LLPVYLLTMSSAVGAADTFEFQVVAPQLGIAHPTGYPLYLLLGKLFSLLPVGTVAWRL
                     NFASAVYATLAAIVVFRLAHDLLDRPLPALAGAVALGLTPVVWSQAIVAEVYTLHALL
                     VAVALGLMLRLVTERRARSTDRRKTVVALAFTIGLGLTNHLTSVFLLPPAALAVALRL
                     WRDRGERQRDLSVQPIMRVLVVVLAFAAPLLLYLYLPLRWRAVNGEPMGAARFVEWVA
                     GGRFQGALQPLAWLRDPARWGIVGRLLLDAWGVVFLLLALVGLAWMLWRAWRAAAVLA
                     LAAAGFLFYALNYYVPDLAVFLIAAHVVVAVFVAAGLTAIVAALEAVGRRARGQAEGV
                     AHWAAAGVFVLALLPLLLAAGGAQANAGHWHALDQSADDGGEPWARVALAGPLAHGAA
                     VLADSEKIAPLYYLQQIEGLRPDLDIMVLPDEAAYRAEVDARLAAGGAVYLARYLPGL
                     AGVYTLRSAGPLVEVSTVPLTALPDDATPASVTVGPLRLLGYQVGAAPDEDAAVAITL
                     FWTQAGPAADSDPSAALHLRWANPFTGAAAPVVSGQYPVGNTYPINAWRPGEIVADYY
                     ILPRPLFDCADAAGCPVALQAAVAPRFGGDAPAWTTLARLPLRPPAGPVAGPAFRAML
                     GAFLLDGATFPPQVRPGTPLAIRYSGYGGGTTPAFALRPAAVADEAAPGGGVISLLPA
                     GASRQYAAELATDLPAGRYRLMARPGTGGAALCGWLSPPRAGCTLGEVEISGAPLPEG
                     AANFDDRAALLAVAVDADGLVPGGQLPVTLTWQGLAPMTENYTVFVQVLDAADRLVGQ
                     VDAWPVQGTFPTSQWTPGEVVRDPYLVRLAAELPPGDYRLHVGLYLLATMARLPVLDA
                     SGAAVDDKVEVPIR"
BASE COUNT         1009 a         2149 c         2042 g          795 t
ORIGIN      
        1 acctggaact cccgctcgcc gccagcggcc ttgtacttgg ccgtgccctt gacgttcggg
       61 tactgcgcgc tacccttgag ggcgatgcgg gtggcgtcgc cgcctttggc gtgggccgcc
      121 ggcgcgaaca taaaggttcc cagaagcaaa gcggccagca aagcagccag aatcgttacc
      181 tttgcccatc ctttcgtcac gtacatgtta gctctcctta tccactgttt ttgatttggt
      241 ttcggtcact ccggcactgc cgtgttgacc aagctatcta acaatgagaa tagcatgtcg
      301 cgcgaagcaa tgaatcgggc caaggttgga aagatcgccg ggccacaggt ctgaattcgc
      361 tccgactttg gtctgatcgc tggatagcca gatgatgcgt tcaaccgctt taagccgtct
      421 gagtctgaca caacagttta tgatcgccag cctcattatc ctggtggctg ccgcgctggg
      481 cctcggccgc tgggcggtaa ggcaaatcga acagagtgtg gtcagccgca cggccgagac
      541 caccgctctc ttcgttgata gcttcatcgc gcccaactta caggagctat cgacctccga
      601 cacactgacg cccgaccaca tcgccaccct cgaccgcctg ctcgacgaca ccccgctcgg
      661 ccggcaaatc ctctccttca aggtctggga cgccaccggc aaggtgctct acagcgctga
      721 gccggccacc atcggccagg tcttccccgt tggcgaggaa ctgacgcgcg cctggagtgg
      781 ccaggtcagc tcgcagatca gcgatctgaa ggacgtcgag aacgcgctgg agcgcaccca
      841 gcgcgcccac ctgctggaga catacgcgcc cgtgcgtttg gagaacagcg acgaaatcat
      901 cgctgtggcc gagttctatc agacagtgga cgaattggag cgcgacatct ccctggccca
      961 ggggcgcacc tggctggtcg tgggcgcgtc gatgttggtg atctacctgc tgttggccgg
     1021 ctttgtcggc cgcgccggcg acaccatcga ccgccagcag gccgccctca gtcgtcaggt
     1081 gaagcagttg accgatctgc tagaccagaa cacagcctta caccagcgcg tccagcgcgc
     1141 cgcggcgcgc acaacggccc tcaacgagcg cgtcctgcgt cgcatcagcg ccgagctgca
     1201 cgacggcccg gtgcaggaca tcggcctggc actgctgcgc ctcgacaatg ttgtcgctca
     1261 ggtggacgac ggtaagactg ccggcgacgg caagaccggc ggcgacgccg cgcccgccat
     1321 cgtccacgac ctggacatgg ttcaggcttc gatgcaacgg gcgctgttgg agattcgcgc
     1381 cctgtcgtcg ggcatgggcg tgccgcaact cggcgcgctg accctgccgg agacgttggc
     1441 gcgggccgtc cgcgttcacc agcagcgcag cggcacagag gtggtgctag acctggacgg
     1501 cacgccggag cgggcctcac tgccggtcaa gatcacgatc taccggctgg ttcaggaggc
     1561 gttgcacaac gcttatcgcc acgccggcgg catcgaccag cgcgtcacgc tctgctacga
     1621 cgccggacgg ctccacattg aggttgccga cggcggcgcc ggcttcggcc aggcggacag
     1681 cgccgccggg gacgaccatc tggggctggc gggcatgcgc gagcgcgtcg agagcctggg
     1741 cgggaacttc cgcgtggaga gcgcccccgg ccagggcacg cgcgtcatcg ccgacctgtc
     1801 gctggccggt gaggaggaag taacatgagc gagaccatcc gcatcgtcct cattgacgat
     1861 catcctctct tccgcgaggg cgtggccatc actctggccg cccagcccga catcgaaatc
     1921 atcggtcagg gcgagaacgt gggcgatgcc gtgcgcctgg cgcgcgaact ggcccccgat
     1981 ctgatgctgc tggacgtcaa tatccccggt ggcggcggcg ctcaggccgc ccagcaggtc
     2041 gccgcggcca acagcgccat tggcatcatc atgctgaccg cctccgacga ggaagacgac
     2101 ttgaccgcgg ccatgaaggc gggcgcgcgt ggctacgtgc tcaagggggt tgccgcgcgg
     2161 gagttggtgg gcatcgtccg ctccgtccac gccggcgagg gctacgtcat gcccgcgctg
     2221 gcggccaggc tcctcagcga catgaccgcc gcccgccggc cgtcgcgcca tgccaacccg
     2281 ctcgacgcgc tgaccgagcg cgagcgccag atcctcgatt gcgtcgccgc cgggctgagc
     2341 aaccgcgaag tgggcgacca gttgcacctg agcgagaaga cggttaagca ctacatgacc
     2401 atcatcatgg agaagctcca ggtgcgcaac cgcgtcgagg cggcactgct cgcgcgcaac
     2461 gcgaccgggc acgacgccaa ttagcccact cactaaaacg gacggctgag gaactggccc
     2521 cagtccagca gcgcctcgcg caacatggcg cggccgcgcc gaatgcgcgc ctcggccgcc
     2581 gccggggcga tgtccagccg gccggcaatg tcgcccggcg acaggccgtg gtaatcggtc
     2641 aggatgagcg cctggcggta ttcccccggc aggcagtcta gtagcccgcg gacggcggag
     2701 cctaacgcag tcacgtcgcc gtcctcgtcc gtcctggcca gggccgccga ttcagaccgt
     2761 tcgacacccg gcggcatggc gcgatagtaa gcggtgattg cgtcccggat gatcgcgtcg
     2821 agccaggttg ccagcccttc actctcgccc agatcgtcgg cgcggctctg aatctcctgg
     2881 ccggcgcgcc acaggatgtc ctcggccgcg ccgggatcag ccccgcgcga caggacgaag
     2941 cgccgcaggt cggcgtgaaa ctgggcgtag atggcctcca gcctcgcggc cgaaaccggg
     3001 gcatcattca ccggctcgcc ctcgtcgcac gccttgcagg ccagagcgac gcccagaaag
     3061 cggctccggc cctcttcggt cagcacccac gcccggtcga cgaacggcgg gttgtggcgc
     3121 acgtgctggc ggtgaccaca gaccagaatc gcccgccaat cgcccaattc atcctgctca
     3181 aagccgataa tcgctctttc cattggcctc accggatagg gacttccacc ttgtcatcca
     3241 ccgccgcgcc gctggcgtcc agcaccggca gccgcgccat cgtcgccaac aggtataacc
     3301 cgacgtgcag ccggtagtcg cccggcggta gctcggccgc cagtcgcacg aggtaagggt
     3361 cgcgcacaac ctcacccggc gtccactggc tggtggggaa agtgccctgc accggccagg
     3421 catccacctg cccgacgagg cgatcggccg cgtcgagcac ctgcacaaag acggtgtagt
     3481 tctcggtcat gggcgccaac ccctgccagg tcagcgtcac cggcaactgc ccgccgggca
     3541 caagcccgtc cgcgtctacg gcgacagcga gcagggccgc acggtcgtcg aagttggccg
     3601 cgccctccgg cagcggcgcg ccgctgattt cgacctcgcc cagagtgcag ccggcgcgcg
     3661 gcggcgacag ccagccgcac agcgccgccc cacccgtgcc cggccgggcc atcagccggt
     3721 agcggccggc cggcagatcg gtggccagct cggccgcgta ttgccgcgac gcgccggcgg
     3781 gcaggagcga gatgacgccc ccgcctggcg cggcctcgtc ggccacagcc gccggccgca
     3841 gagcaaatgc cggcgtcgtc ccgccgccgt agccactgta gcgaatcgcc aggggcgttc
     3901 ccggccgcac ctgcggcggg aacgtcgccc cgtcgagcag gaacgcgccc aacatggccc
     3961 ggaacgccgg ccccgctacc gggccggccg gcgggcgcag cggcagccgg gccagtgtcg
     4021 tccacgccgg cgcgtcgccg ccaaagcgcg gcgcgacggc ggcctgcaac gccacggggc
     4081 agccggccgc gtcggcgcag tcgaagagcg gccgcggcaa gatgtagtag tcggccacga
     4141 tttcgcccgg ccgccaggcg ttgatcgggt aggtgttgcc gacagggtat tggccgctga
     4201 cgacgggcgc tgcggccccg gtgaaggggt tggcccagcg cagatgcagc gccgccgacg
     4261 ggtcgctatc cgccgcgggg ccggcttgcg tccagaagag ggtgatggcc acggccgcgt
     4321 cttcgtctgg cgcggccccg acctggtagc ccagtaagcg caacgggccg acagtcacgc
     4381 tggccggcgt ggcgtcgtcg ggcagggctg tcagcggcac cgtgctcacc tcgaccagcg
     4441 gcccggccga gcgcagggtg tagacgccgg ccagaccggg cagatagcgg gccaggtaga
     4501 ccgccccgcc cgcggccagc cgggcgtcca cctcggcccg gtaggcggcc tcgtcgggca
     4561 gcaccatgat gtcgaggtcg ggccgcagcc cttcgatctg ttgcaggtag tagagcgggg
     4621 cgatcttctc gctgtcggcc agcacggccg cgccgtgggc cagcggcccg gccagcgcca
     4681 cccgtgccca cggttcgcca ccgtcgtcgg ccgactggtc gagcgcgtgc cagtggccgg
     4741 cgttggcttg cgccccgccg gcggcgagga gcagcggcag cagggccagg acgaagacgc
     4801 ccgcggccgc ccaatgggcg acgccctccg cttgcccgcg cgcccgccgg cccacggcct
     4861 ccagcgcggc gacaatggcc gtcagcccgg cggcgacgaa cacggccacg accacgtgcg
     4921 cggcgatgag gaagacggcc agatcgggca cgtagtagtt gagagcgtag aagaggaagc
     4981 cggccgcggc cagcgccagc actgccgccg cccgccatgc ccgccacagc atccacgcca
     5041 gcccaaccag cgccaacagc aggaagacga cgccccaggc atccagcagc aaccgcccga
     5101 cgattcccca gcgggccggg tcgcgcagcc aggccagtgg ctgcaacgcc ccctggaagc
     5161 gcccgccggc gacccattcg acgaaccgcg ccgcgcccat cggctcgccg ttgaccgccc
     5221 gccagcgcag cggcaggtac aggtagagca gcagcggcgc ggcgaaggcg aggacgacca
     5281 ccagcacgcg catgatcggc tgcacggaca gatcgcgctg ccgctcgccg cggtcgcgcc
     5341 acagccgcag ggcgacggcc aacgccgccg gcggcagcag gaagacgctg gtcaggtggt
     5401 tggtcagccc caggccgatg gtgaaagcca gggctacgac tgtcttgcgc cggtcggtgg
     5461 agcgcgcccg gcgctccgtc accagccgca gcatcagccc cagcgccacg gcgacgagca
     5521 gcgcgtgcag cgtgtagacc tcggcgacga tggcctggct ccacaccacc ggcgtcagcc
     5581 ccagcgccac cgccccggcc agcgcgggca acggccggtc gagcaggtcg tgggccagac
     5641 ggaagacgac gatggccgcc agcgtggcgt agacggccga ggcgaagttg agccgccagg
     5701 cgaccgtgcc cacgggcagc agcgagaaca gcttgcccag cagcaggtag agcgggtagc
     5761 cggtggggtg ggcgatgccg agttgtgggg cgacgacctg gaactcgaac gtgtccgccg
     5821 cgccgacggc cgaggacatg gtcagcaggt agacgggcag cagcgccgcg gcaacgaacg
     5881 ccggccccag gcgcagccag tgccggtgac cggggccaag ccgcagccac gcccagacga
     5941 cggcggccag ccaccagccg gcggcgaaga gaaagcgccc gcggggcaag tccac
//
LOCUS       DAOSMQ010000158         5962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13463_length_5962_cov_5.595734, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000158 DAOSMQ010000000
VERSION     DAOSMQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13463_length_5962_cov_5.595734"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK829_06720"
     CDS             <1..459
                     /locus_tag="PK829_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOU40944.1"
                     /translation="ANGVPVMPGLGALLDALDARGLPWAVATSTPRDVAAHILGQMGL
                     AGRYGALAGGDEVTRGKPSPELYLLAAARLGVNPAACLALEDTPIGCEAAAAAGARVL
                     AVPTDMTRAAAFPCAYRRYPSLAEVAADLDGLLLGARGQGLGKNALPPAT"
     gene            668..2707
                     /gene="nusA"
                     /locus_tag="PK829_06725"
     CDS             668..2707
                     /gene="nusA"
                     /locus_tag="PK829_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOU40945.1"
                     /translation="MKSEFILAFNEICESRGLPKEDVFEALKTALVSAYRRDANLSSN
                     QHVTVEIDPRTGEPTIFTEKEVVDDVMDNRTEVTLTAARQEGHTTAELGDVVMVDSTT
                     ESFGRIAAQTAKQVLLQRVREAEREHLFEDFSGREGELVNGTVQSISGQHITIGLGRT
                     EAMLPKSQQVQGERYRAHDKIRVYVLEVRRTSRGPQIVVSRNHRNLLRRLLELEVPEI
                     YNGQVEIKSIAREAGQRSKVAVQALQHGVDPVGACVGMRGVRIQSIVRELNDEKIDVI
                     EWDGDQRVFIAKALSPARVSHVFLEEHPEEGKTAVVIVPDDQLSLAIGREGQNARLAA
                     KLTGWRIDIKNLTEAASEALNNLDHPSVDPRIAQDTTLMNQVANILDKKQTGRPITAE
                     DYQTLDRLVANIEGRIMAQRAQEFDVVRKQRAELRKRVPETAWEQPLDVLDLPGRIHN
                     LLLDTNVNTVGDLIYILEMGDDYFLKLRGLGEKALETVKDTLAAYQSAQIDAVAAAEA
                     AGMAPVESIDEVTPAMPEEVVEEVLQVEELPIIDRIYQMEDMVTAVPDIILPEEEELA
                     FDDETLDRMEIAGVLSLDEVEPEVEETLTPIDDLSATIFVKEEAPAKPEPKKKPAVVV
                     VRPAAEETAEEEAKRKKRKGQPLVYNEELNQVVVDRKRKGGKRPAWEDEDLGVDI"
     gene            2712..3032
                     /locus_tag="PK829_06730"
     CDS             2712..3032
                     /locus_tag="PK829_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOU40946.1"
                     /translation="MTGQGSGHSPRRARHVPQRTCVVCRRQVDKRRLTRVVRTADSGV
                     LIDPTGKRNGRGAYLCDQPSCWERATRQAAILNQALNAQVTEAELAAIAADPRRPAPA
                     QAGS"
     gene            3109..>4624
                     /gene="infB"
                     /locus_tag="PK829_06735"
     CDS             3109..>4624
                     /gene="infB"
                     /locus_tag="PK829_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU40947.1"
                     /translation="MTVRELATLMGVSPINVIKELMANGIMANINQQIDFETAAIVAG
                     EMGYDVVAHEEAAPEPVAEEAKVGWRKVLADERSSDLQPRPPIVTMLGHVDHGKTSLL
                     DVVRQTNVTAGEAGGITQHIGAYQTVKDGRLVTFLDTPGHEAFTAMRARGAQATDIAI
                     LVVAADDGVMPQTREAVDHARAAHVPIVVALNKIDLPGARPDRVKQQLSEMGLAPDDW
                     DGDTMVIPVSAREKLGIDDLLEAVLLVAEEVDPRANPTAPAAGTVLEAKIERGKGIMT
                     TALVQNGTLHLGDTMLVGEHYGRIKAMYDYTGKRIKEAGPSTPVSVSGLDGIPEAGEQ
                     FRIVDSEKTARRIIDEEREAARVTPTSRGRGPTLDEFFARLVEGEVKTLNLIVKADVQ
                     GSLEPIVTSLERLDGGEVELDILRAATGPITESDVMLASASGAIILGFNVDADPIARN
                     AAAVEEVQIRTYTIIYKLIEDVEKAMKGMLAPTFEEVVIGRAEVRQVFRIRGVGN"
     assembly_gap    4625..4724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4725..5009
                     /locus_tag="PK829_06740"
     CDS             <4725..5009
                     /locus_tag="PK829_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU40948.1"
                     /translation="VIGRAEVRQVFRIRGVGNIAGSYMRTGEGRRNARARVVRNSRLL
                     HSGTISSLKHLQENVREVKAGFEFGVSLDGFEDFQPGDIIEFFVSQRVEA"
     gene            5040..5507
                     /gene="rbfA"
                     /locus_tag="PK829_06745"
     CDS             5040..5507
                     /gene="rbfA"
                     /locus_tag="PK829_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU40949.1"
                     /translation="MSKIRQERTAELIQQLLSEVFLREVSDPRLAGVTVTEVQIDREL
                     QYATAFVNALGGDEQRDEVMAGLQSASGFLRHTLAQGLDLRTAPILRFEWDPRLRYVE
                     EVDELLNELNIKPEEAVATSAALMAAATPADDQPSADRPAADRPAADDLDEEE"
     gene            5536..>5962
                     /locus_tag="PK829_06750"
     CDS             5536..>5962
                     /locus_tag="PK829_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOU40950.1"
                     /translation="MIQFAVEKANRVLVIAHIDPDGDAIGSLTATGQALQQLGKRVTL
                     ACDDSVPQRFHYLPLAGDVRRAPNPDANYDLVVAVDCADESRMGWAYACLPEPKPTLA
                     NIDHHITNTRFGAFNIVEPAASSSAEILCRFLADIGATMT"
BASE COUNT         1139 a         1910 c         1934 g          879 t
ORIGIN      
        1 gccaacggcg tgccggtgat gcccggtctg ggggcgctgc tggacgcgct ggacgcgcgc
       61 ggcctgccct gggccgtggc cacgtcaacg ccgcgcgacg tggccgccca catcctgggt
      121 cagatgggcc tggccgggcg ctatggggcg ctggccggcg gcgacgaggt cacgcggggc
      181 aagccatcgc ctgagttgta cctgctggcc gctgcgcggt tgggcgtcaa cccggcggcc
      241 tgcctggcgc tggaggacac gcccatcggt tgcgaggcgg ccgcggccgc cggggcgcgc
      301 gtcctggccg tgcccacgga catgacccgc gccgccgcct tcccctgcgc ctatcgccgc
      361 tacccgtcgc tggccgaggt ggccgccgat ctggacgggc tgctattagg ggctaggggc
      421 caggggctag ggaagaacgc tcttccccct gcaacctgac ccttggaacc tgacctccta
      481 gcccccaacc cctaacccct agccccttgt cactttgcgg ccatcgtgct atactccctt
      541 cactacagtc tgcgggcgag cgagcgatgg aaaaagtggg cacccccact ttttttgttt
      601 cttgcttgga aggtctgtgg cgattgtact gtagataaaa actgaatagg tggttggaac
      661 gagttccatg aaaagcgaat ttattctggc gttcaacgaa atctgtgagt cccggggtct
      721 ccccaaagag gacgtcttcg aagccctgaa gacagccctc gtttccgcct accgacgcga
      781 cgcgaacctg agcagcaacc agcacgtcac tgtggaaatc gacccgcgca ccggcgaacc
      841 aacgatcttc accgagaaag aggtcgttga cgatgtgatg gacaaccgca ccgaggtcac
      901 gctgaccgcg gcgcgccaag agggccacac caccgccgaa ctgggcgacg tggttatggt
      961 cgatagcacc acggagagct tcggccgtat tgccgcgcag acggccaagc aggtcttgct
     1021 ccagcgcgtc cgcgaggccg agcgcgagca cctcttcgag gacttcagtg gccgcgaggg
     1081 cgagctggtc aatggcacgg tgcagagcat cagcggccag cacatcacca tcggcctggg
     1141 ccgcaccgaa gccatgctac ccaagagcca gcaggtgcag ggcgaacgct accgcgccca
     1201 cgacaagatt cgcgtctacg tgctcgaagt gcgccgcacc agccgcggcc cgcagatcgt
     1261 cgtcagccgc aaccaccgca atctgctgcg ccggctgcta gagttggaag tgccggagat
     1321 ctacaacggc caggtagaga tcaagagcat cgcccgcgag gcgggccagc gctccaaggt
     1381 cgccgtccag gcgttgcagc acggcgttga ccccgtcggc gcgtgcgtcg gcatgcgcgg
     1441 cgtccgcatc cagagcatcg tccgcgagtt gaacgacgag aagatcgacg tcatcgagtg
     1501 ggacggcgac cagcgcgtct tcatcgccaa ggccctcagc ccggcgcgcg tctcgcatgt
     1561 ctttctggag gagcacccgg aggaaggcaa gacggccgtg gtcatcgtgc ccgatgacca
     1621 gttgtcgctg gccattggcc gcgagggcca gaacgcccgt ctggccgcca agctgaccgg
     1681 ctggcgcatc gacatcaaga atctgaccga ggccgcctcc gaggcgctca acaacctcga
     1741 ccacccgtcc gtcgatccgc gcatcgccca ggacacgacg ctgatgaacc aggtggccaa
     1801 catcctggac aagaagcaga ccggtcggcc catcaccgcc gaggattacc agacgctcga
     1861 ccggctggtg gccaacatcg aggggcgcat catggcccag cgggcacagg agttcgacgt
     1921 ggtgcgcaag cagcgcgccg agttgcgcaa gcgcgtgccg gagacggcct gggagcagcc
     1981 gctggacgtg ctcgacctgc ccggccgcat ccataacctg ctgctagata caaacgtcaa
     2041 caccgttggc gacctgatct acattctgga gatgggcgac gactacttcc tgaagctgcg
     2101 cggtctgggc gagaaggcgc tggagacggt caaggacacg ctggccgcct accagtcggc
     2161 gcagattgac gccgtggccg cggccgaggc ggccgggatg gcccccgtcg aatcaatcga
     2221 cgaggtcacg ccggccatgc cggaggaagt cgtcgaggaa gtgctgcaag tcgaggaact
     2281 accgatcatc gaccgcatct atcagatgga agatatggtc acggccgtgc ccgacatcat
     2341 cctgcccgaa gaggaggaac tggccttcga cgacgagacg ctcgaccgga tggaaatcgc
     2401 cggcgtgctg tcgttggacg aggtcgagcc ggaagtagag gagacgctga cgcccatcga
     2461 cgacctgtcg gccaccatct tcgtgaagga agaggccccg gccaagccgg agccgaagaa
     2521 gaaaccggcc gtcgtcgtcg tgcggcccgc cgccgaagag acagccgagg aagaggccaa
     2581 gcgcaagaag cgcaagggcc aaccgttggt ctataatgag gaactgaatc aagtcgtcgt
     2641 cgaccgcaag cgcaagggcg gcaagcgccc ggcctgggaa gacgaagacc tgggtgtgga
     2701 tatctaaaac gatgaccgga cagggatcag ggcactcacc gcgccgtgcg cgccacgtgc
     2761 cccagcggac gtgcgtcgtc tgccggcggc aggtggacaa gcgtcgcctg acgcgcgtgg
     2821 tacgcacggc cgacagcggc gtcctgatcg acccgaccgg caagcgcaac ggccgcgggg
     2881 cctatttgtg cgaccagccg tcgtgttggg agcgcgcgac caggcaggcg gcgatactca
     2941 atcaggcgct gaacgcgcag gtgactgagg cggagttggc ggctatcgcc gctgacccgc
     3001 ggcggccagc gccggcccag gccggatcat gagcggacag ttttgttttg ggcggaaagg
     3061 cagtaaggta tggcagagac aagaacacta atcgagattc cggcattcgt gaccgtacgc
     3121 gagctggcga cactgatggg cgtgagtccg atcaatgtca ttaaggagtt gatggccaat
     3181 ggcatcatgg ccaatatcaa ccagcagatc gacttcgaga ccgcggccat cgttgccggc
     3241 gaaatgggct atgatgtcgt cgcccacgaa gaggccgctc ccgaaccggt tgccgaagag
     3301 gccaaggtcg gctggcgtaa ggtgctggcc gacgagcgca gtagcgacct tcagccgcgg
     3361 ccgcctatcg tcaccatgct cggccacgtt gaccacggca agacctcgct gctggacgtg
     3421 gtgcgccaga ccaacgttac tgccggcgaa gcgggcggca ttacccagca catcggcgct
     3481 taccagacag tcaaggacgg ccggctggtg accttcctgg atacgcccgg ccacgaggcc
     3541 tttacggcca tgcgtgcccg cggggcgcag gcgaccgaca tcgccatcct ggtggtggcc
     3601 gctgatgacg gcgttatgcc gcaaacgcgc gaagccgtcg accacgcccg cgccgcccat
     3661 gtgcctatcg tcgtcgccct gaacaagatc gatctgcccg gcgcgcgccc cgaccgcgtt
     3721 aagcagcagc tcagcgaaat gggcctggcg cccgacgact gggacggcga cacaatggtc
     3781 attcccgtct cggcgcgcga gaagctgggc attgacgacc tgctggaggc ggtgctgttg
     3841 gtggccgaag aggtggaccc gcgcgccaac ccgaccgcgc cggccgcggg cacggttttg
     3901 gaagccaaga tcgagcgcgg caagggcatc atgaccacgg ctctcgtcca gaacggcacg
     3961 ttgcatctgg gcgacacgat gctggtcggc gaacactatg gccgcatcaa ggccatgtat
     4021 gactacaccg gcaagcgcat caaggaagcc ggcccgtcga cgccggtgtc cgtgtcgggc
     4081 ctggacggta tcccggaggc gggcgagcag ttccgtatcg tggatagcga gaagactgcc
     4141 cgccgcatca tcgacgagga gcgcgaggcc gcccgcgtga cgcccaccag ccgcggccgc
     4201 ggcccgacgc tggacgaatt cttcgcccgc ctggttgagg gcgaggtcaa gacgcttaac
     4261 ttgatcgtta aggccgacgt gcaaggctcg ctggagccga tcgtcacctc gctggagcgg
     4321 ctggatggcg gcgaagtcga gcttgacatc ctgcgcgccg ccaccggccc catcaccgag
     4381 agcgacgtca tgctggcctc ggcgtccggg gccattatcc tgggcttcaa cgtcgatgcc
     4441 gacccgatcg cccgcaatgc cgccgctgtc gaagaggtgc aaatccgcac ctacacgatc
     4501 atctacaagc tgatcgaaga cgtggaaaag gccatgaagg gcatgttggc tccgaccttc
     4561 gaggaggtag tcatcggccg ggccgaagtg cgccaggtgt tcaggattcg cggcgtgggc
     4621 aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtcatc ggccgggccg
     4741 aagtgcgcca ggtgttcagg attcgcggcg tgggcaacat cgccggcagc tatatgcgca
     4801 cgggcgaggg gcggcgcaac gccagggccc gcgtcgtccg caacagccgc ctgcttcaca
     4861 gcggcacgat cagtagcctg aagcatctgc aagagaacgt gcgcgaggtg aaggcgggct
     4921 tcgagttcgg cgtcagcctg gacggcttcg aggacttcca gcccggcgac atcatcgagt
     4981 tcttcgtttc ccagcgcgtt gaggcgtagc tataagcaag aagctatgag caagaagcta
     5041 tgagcaagat aagacaagag cgcacggctg aactcatcca acagcttctc agtgaggtct
     5101 tcctgcgcga ggtcagcgac ccgcgtctgg cgggcgtgac cgtcaccgaa gtacagatcg
     5161 accgcgagtt gcagtatgcc acggccttcg tcaacgccct gggcggcgac gagcagcgcg
     5221 acgaggtcat ggccggcttg cagagcgcct ccggcttcct gcggcacaca ttggcccagg
     5281 gtctggacct gcgcaccgcg ccgatattgc gtttcgagtg ggacccgcgc ctgcgctacg
     5341 tcgaagaggt ggatgagctg ctcaacgagc tgaacatcaa gccggaagag gccgtagcca
     5401 cgtcggcggc gctgatggcc gcggcgacgc cagccgatga ccaaccgtcg gccgaccggc
     5461 ccgcagccga ccggcctgca gccgacgacc tcgacgaaga agagtaggca cggccacagc
     5521 cgacaggggg agccaatgat ccagttcgcc gtcgaaaaag ccaaccgcgt gctggtcatt
     5581 gcccacatcg accccgacgg cgacgccatc ggctcactga cggccacggg ccaggcattg
     5641 cagcagcttg gcaagcgcgt cacgctggcc tgcgacgaca gcgtgccgca gcgcttccat
     5701 tacctgccac tggccggcga cgtgcgccgc gcgcccaacc cggacgccaa ctacgacctc
     5761 gtcgtcgctg tcgattgcgc cgacgagtcg cgcatgggct gggcctacgc ctgtctgccg
     5821 gagccgaagc cgacgctggc caacatcgac caccacatca ccaacacccg cttcggcgcg
     5881 ttcaacatcg tcgagccggc ggccagttcc tcggccgaaa tcctctgtcg cttcctggcc
     5941 gacatcggcg cgacgatgac cc
//
LOCUS       DAOSMQ010000159         5936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13542_length_5936_cov_4.954600, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000159 DAOSMQ010000000
VERSION     DAOSMQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13542_length_5936_cov_4.954600"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PK829_06755"
     CDS             complement(<1..156)
                     /locus_tag="PK829_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40951.1"
                     /translation="MDIRPMTLSDYDAVMALMSGVPGVAVRTADSPPAIGRYLERNPG
                     LSLVAEDG"
     gene            complement(128..613)
                     /locus_tag="PK829_06760"
     CDS             complement(128..613)
                     /locus_tag="PK829_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40952.1"
                     /translation="MDRELTLARVAFRALGDEWHDLVRAGYHDDYNGMGELYSGVTTV
                     EDKIAQMQRWGEGIDAWTARDDGRLAGILTAAVDDDTLTVYDFFVDPAYQRRGIGRTL
                     LTTALATPGVRQAAAEINLANAASRRLFESLGFAPQQTVGWYIRAGEEGSDGHQADDT
                     E"
     gene            complement(615..1379)
                     /locus_tag="PK829_06765"
     CDS             complement(615..1379)
                     /locus_tag="PK829_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40953.1"
                     /translation="MSAVATYREHLRQMCDWESFLLAESRLPGPRANLDLAQAAADEG
                     DATQFRAWLAIGADEAPANTPGEFLPLCGALGFGRLLAEGDRAALADIRRAANDRRWR
                     VREGAAMALQRWGAADMPALLAEADDWAGGTRLEQRAIAAALCEPPLLTDVATAERVL
                     DVLDRITATVRGAAAVSGTAERRSDGFVALRKGLGYCWSVAVVAAPARGQALMEKWLA
                     DEDKDVRWIMRENLGKARLARLDADWLARQRARLEG"
     gene            complement(1379..1675)
                     /locus_tag="PK829_06770"
     CDS             complement(1379..1675)
                     /locus_tag="PK829_06770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU40954.1"
                     /translation="MATDPDGELAAFVIGWQHTTAGGRLIGMVEPFGVREDARRYGLA
                     WALMFELLRRFDAAGVAEVRVMTDNYRDTAFAFYSGCGFQVIDRVLMLGKKVDD"
     gene            complement(2290..2883)
                     /locus_tag="PK829_06775"
     CDS             complement(2290..2883)
                     /locus_tag="PK829_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOU40955.1"
                     /translation="MTNPYDYSNRAGVRPISWDDFHGLVKALAVAVASWRPEVVLPVL
                     RGGAYPGALLAHILQVEVYPVRLTRRQDDVVVRESPLWLVEPPAAVAGRRVLVVDEMC
                     STGETLRLVRERALALDAAEARTATLYAHTWGADAVDYTGLITDELVLNPWDREIYRD
                     GAFQFHPEYVSALAEQGLVAEPALLIAATPFVAARGS"
     gene            complement(3718..4533)
                     /locus_tag="PK829_06780"
     CDS             complement(3718..4533)
                     /locus_tag="PK829_06780"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOU40956.1"
                     /translation="MTSAMPTDAAARPDRARHLDWEPCWNVRDLGGLPLRGGGETRWG
                     ALIRADCLGKLTTAGRQALIDYGVGTIIDLRSADELAIDPPAFPGGRAGEPAFRHAPL
                     VTNDPAANARWMALQTRADVYRIFLDFYGPGLAGALRAIAGAPPGGVVFHCLGGTDRT
                     GLVAALVLRLAGVPDEVIAADYEMTLARRRSIYAQLVAEKGEAALGFWDRLNMTADNM
                     LATLAYLDAQHGGERAYLRANGLADGDAERVRARLRWNASPLLGGEGTRTLSR"
     gene            complement(4669..>5936)
                     /locus_tag="PK829_06785"
     CDS             complement(4669..>5936)
                     /locus_tag="PK829_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOU40957.1"
                     /translation="IRNVGLSHLDFLEISQPGGAFTVTPMNCLGGLDAGESCTLSLVF
                     KPLAAGGAAATVVVNTTGGQAQLHLSGFGRASGDAIGQQVTPTELDFGPVQIGAASES
                     QRVTFTNQSSTAHLINWSGGGTTAPFSKTTNCQDDIDPATSCYYDFTFSPTAAGAFSQ
                     TVRVTNNVGTVEIVLHGEGVAPRIIADAMALEFPPVAPGQTSPPQIVTVRNAGLAPVQ
                     MFGGAPNPGDIFGASTTCGTLMPPGATCQVFYTFEPDTFGRLSATSTFSLDAQGHFPL
                     TLRLTGGAGMPGLALSFAPAAVDPGEVTRLQIALANSNPSLALLNVSLLTQLPPGLVI
                     ADPPEPSVGSDCRGGTFQPVAGSRAVVLSGGMLGGQTCTMSVRVVATTRGRYTVSATA
                     TSDSGPSNSASATLGVGTGYTVNLPFVRR"
BASE COUNT          899 a         2199 c         1964 g          874 t
ORIGIN      
        1 gccgtcttcg gccaccagac tcagccccgg gttgcgctcc aggtaacggc cgatggccgg
       61 cggcgaatcg gcggtgcgca cggccacgcc gggcacgcca ctcatgagcg ccataacggc
      121 gtcgtaatca ctcagtgtca tcggcctgat gtccatcgct gccttcctcg ccggcgcgga
      181 tgtaccagcc gaccgtctgc tgtggcgcaa agcccagcga ctcgaacagc cgccgtgagg
      241 cggcgttggc caggttgatc tctgccgcgg cctggcgcac gcccggcgtg gccagcgccg
      301 tcgtcagcag cgtccggccg ataccccgcc gctggtaagc cggatcgacg aagaaatcat
      361 agaccgtcag ggtgtcgtcg tcgacggcgg cggtcaggat accggccagt cggccgtcat
      421 cccgcgccgt ccaggcgtcg atgccctcgc cccaacgctg catctgggcg atcttgtcct
      481 cgaccgtcgt cacgccgctg tacagctcgc ccatgccgtt gtagtcgtca tggtagccgg
      541 cgcgcaccag gtcgtgccac tcatcgccca gcgcccggaa ggccacgcgg gcgagcgtca
      601 attccctgtc catatcagcc ctccagtcgc gcgcgctgcc gcgccagcca gtcggcatcc
      661 agccgcgcca gtcgcgcctt gcccaggttc tcgcgcatga tccagcgcac gtccttgtcc
      721 tcgtcggcca gccacttctc catcagcgcc tggccccgcg ctggcgcggc caccacggcg
      781 acgctccagc agtagcccag ccccttgcgc agggcgacga agccgtcgct gcgccgttcg
      841 gccgtgccgg acacggcggc cgcgccgcgc accgtggccg taatgcggtc gagcacgtcc
      901 agcacgcgct cggccgtggc cacgtcggtc agcagcggcg gctcgcacag cgccgcggcg
      961 atggcccgct gctccagccg cgtgccgccg gcccaatcgt cggcctcggc cagcagcgcg
     1021 ggcatgtcgg ccgcgcccca gcgttgcagg gccatggccg cgccctcgcg cacgcgccag
     1081 cgccggtcgt tggccgcccg gcggatgtcg gccagcgccg cgcggtcgcc ctcagccagc
     1141 agccggccga agcccagcgc gccgcacagc gggagaaact cgcccggcgt attggccggg
     1201 gcctcgtccg cgccgatggc cagccaggcg cggaactgcg tcgcgtcgcc ctcgtcggcc
     1261 gccgcctggg ccaggtcaag gttggcccgc ggccccggca ggcgcgactc cgccagcagg
     1321 aacgactccc agtcacacat ctggcgcagg tgttcgcggt acgtcgccac agcgctcatc
     1381 agtcgtccac ctttttgccg agcatcagca cccggtcgat cacctgaaag ccgcaaccgc
     1441 tgtagaaagc aaaggccgtg tcgcggtagt tgtcagtcat gacgcgcacc tcggccacgc
     1501 cggcggcgtc gaagcgtcgc agcaactcga acatcagcgc ccacgccaac ccatagcgcc
     1561 gcgcgtcctc gcgcacgccg aacggctcga ccatgccgat gagccggccg ccggccgtcg
     1621 tgtgctgcca gccgatgaca aacgcggcca attcgccgtc cgggtcggtg gccaccagat
     1681 cgaactccgg ccggtagccg ggcgcgcgca gcgtcgccgc ccgccagtcc ggtcatgttc
     1741 tccgtgccga agacggcgcg gtgcaaagcg acgtaggcca acacttcggc cgcgccgcgc
     1801 agtggccgga tggcgaagcc tggccgcagg gccacagccg gcggcggctc ctcgccggcg
     1861 cgccgcatga tcacctgtga ccacggttcg tcgccgccgc tctggtcgct gtagccggcg
     1921 gcggcgaaga cccgccggcg ctcgccctgc ccctccggca cgccggcgaa ccacaccggc
     1981 cggccgtaag gcgtggctcg caaccgcgcc gccgcgccgt cggcccaggc caacagcgcc
     2041 gccagcgtat ccgccggcgc ggccgggtgc agggcgtaat cgagcgtcca gaacggcgct
     2101 tgtaacgcgg cccaggccag cagttcgccg tcccgctccc aaagggccgt cgtctccggg
     2161 caatcgaacg cccacgaggc cagccggtag ggcaaatcga cgacgtgaac cgtctccccg
     2221 gctgccagcg tcgccagcgc cagcatccgc gcccggtcgt cgtggccacg atacgctctc
     2281 ggttccatcc tagctccccc gggcggccac aaacggcgtc gccgcaatca gcagcgccgg
     2341 ctcggcgacc aacccctgct ccgccagcgc gctgacgtac tccgggtgga actggaacgc
     2401 gccgtcgcgg tagatctcgc ggtcccacgg gttcagcacc aactcgtcgg tgatgaggcc
     2461 ggtatagtcc acggcgtccg cgccccaggt gtgggcgtag agcgtcgccg tccgcgcctc
     2521 ggccgcgtcc agggccagcg cccgctcgcg caccagccgc agcgtctcgc ccgtgctgca
     2581 catctcgtcg accaccagca cgcgccgccc ggccacggct gccggtggct ccaccagcca
     2641 cagcggcgac tcgcgcacga caacgtcatc ctgtcgccgc gtcaggcgca ccgggtagac
     2701 ctccacttgc aggatgtggg ccagcagcgc gccggggtaa gccccaccgc gcaacaccgg
     2761 cagcaccacc tccggccgcc acgaagccac ggccacggcc agcgccttca ccagcccgtg
     2821 aaagtcgtcc caggagatcg gccggacgcc ggcgcggttg gagtagtcgt aggggttggt
     2881 catgccgcta gtctagcgca gaggtatatt tgtgtgccag tttgacaatg cctgggtcta
     2941 ccgctttcca cgcacggacg agtcgcgcgc gtctggctga agaagttaga ttactggccg
     3001 ttgttcgaca tgatgtcaat gtgcccgtac ccgcctttga gaccgacgag atacttgatt
     3061 tcgtcgccta ccggctgctg cccggacgtc cgctctaccg ccacgacctg ctacgcctac
     3121 cccaccaggt gcaaaaccaa ttcgccgccg aggtggccgc ttttttggaa caactgcacg
     3181 ccatccccac taccgacccg gtcgatttac cgccgcggcc agccctcaac agcgcctcgc
     3241 cgtggcaaga gcgattggaa gtgctgcgag cagagatgta cccgtacctt tgggccgacc
     3301 agcacgcgta tatcgaggat cttttcgctc ctgtgctcga cggccgcctc gacatggcct
     3361 acacgccagt gcttatccac aacgatctcg cgccctatca cttgctggcc gacccggtga
     3421 cgggcacatt gaccggtgtg ctcgatttcg gggaggcagg ctggggcgac ccggccgccg
     3481 actacgccgc gctcatcagc agctacggcg agtcgctcgt caaccgggtg ctggccacca
     3541 cgccggacgc cgccccccta ctggagcgcg cccgctttcg cgccgcctac gtcgaattgg
     3601 aatgggcctt gaagggtgtc cgcactgctg accccagctg gtatcttgtc caccttggcc
     3661 gggcacggga tgcacagcca ttccgggccg cgtcagtgaa cacgttggct ctccctccta
     3721 ccgggagagg gttcttgtcc cctctccccc gaggagagga gatgcattcc agcgcagccg
     3781 cgcccgaacg cgctccgcgt ctccgtccgc aaggccgttg gcgcgcaggt aggcccgttc
     3841 gccgccgtgc tgcgcgtcga gataggccag cgtggccagc atgttgtcgg cggtcatgtt
     3901 gagccgatcc cagaagccca gggcggcctc gcccttttcg gccaccagtt gggcatagat
     3961 cgaccgccgt cgcgccagtg tcatctcgta gtcggcggcg atgacttcat cgggcacgcc
     4021 ggccagccgc aacaccaacg ccgccaccag tccggtgcgg tccgtcccgc ccaggcagtg
     4081 gaagacgacg ccgcccggtg gcgcgccggc aatggcccgc agagcgccgg ccaggccagg
     4141 gccgtagaag tcgaggaaga tgcgatagac gtcggcccgc gtctgcaagg ccatccaacg
     4201 ggcgttggcg gccgggtcgt tggtgaccag cggcgcgtgg cggaaggccg gctcgcccgc
     4261 ccggccgccg gggaaggccg gtgggtcgat ggccaactcg tcggccgagc gcaggtcgat
     4321 gattgtgccc acgccgtagt cgatcagcgc ctgccgcccg gctgtggtca gcttgcccag
     4381 gcagtcggcg cggatgagcg cgccccaacg ggtctcgccg ccgccacgga gcggcagccc
     4441 gcccaggtcg cggacgttcc agcagggttc ccagtcaaga tgacgcgccc tgtcaggacg
     4501 cgcggcggca tcggtcggca ttgcggaagt caacagtgac tctcctgagt gagcgattgc
     4561 cggccggggc ggccggcgcg gtcggtcaag cccgcgccag ccacgccccg gccggccctc
     4621 cattatggcc gcgcgcgcgt ctctcgccaa ctcgccgccg ccggtcggct agcggcggac
     4681 gaagggaaga ttgactgtat agcccgtgcc gacgcccaac gtcgccgacg cgctgttgct
     4741 ggggccgctg tcgctggtgg ccgtggcgct gacggtgtag cggccgcggg tagtggcgac
     4801 gacgcgcacg ctcatcgtgc acgtctgccc gcccaacatg ccgccgctga gcaccaccgc
     4861 ccgcgagccg gccaccggct ggaacgtgcc gccgcgacag tcgctgccca ccgacggctc
     4921 gggcgggtcg gcgatgacca gccccggcgg cagttgcgtc agcaggctga cgttcagcag
     4981 cgccagcgac gggttgctgt tggccagggc gatttgcagc cgggtcacct cgccaggatc
     5041 gacggccgcc ggcgcgaagc tgagcgccag ccctggcatc cccgccccgc ccgtcaaccg
     5101 gagcgtcagc gggaagtgcc cttgggcatc cagggagaac gtcgatgtgg cgctaagacg
     5161 gccgaacgtg tccggctcga aggtatagaa gacctggcac gtcgcccccg gcggcatcag
     5221 cgtgccgcat gtggtcgacg cgccaaagat gtcgcccggg ttgggtgctc cgccgaacat
     5281 ctgcaccggg gccaggcccg cgttgcgcac cgtcacgatc tgcggcgggc tggtctggcc
     5341 gggagccacg ggcgggaact ccagcgccat cgcgtcggcg atgatccgcg gcgcgacccc
     5401 ctcgccgtgc aagacgattt ccaccgtgcc gacgttgttg gtcaccctga ctgtctggct
     5461 gaacgccccc gccgccgtcg ggctaaaggt gaagtcataa tagcaggagg tcgccgggtc
     5521 gatatcgtcc tggcaattgg tcgttttgga aaacggcgca gtcgttccgc cgccagacca
     5581 attgatcaga tgggcggtgc tgctctggtt ggtgaacgtc acccgttgcg actcagaggc
     5641 cgcgccgatc tgcaccgggc cgaagtcaag ctcggtcggc gtcacctgct ggccgatggc
     5701 gtcgccggac gcgcggccga agccggacag atgcaattgc gcctggccgc ccgtggtatt
     5761 gacgacaacc gtggccgccg ccccgccggc ggccagcggt ttgaacacca gcgagagggt
     5821 gcaactctcg cccgcgtcca gcccgccgag gcagttcatc ggcgtgaccg tgaacgcccc
     5881 accgggctgg ctaatctcca ggaagtcgag gtggctcagg ccgacgttgc ggatga
//
LOCUS       DAOSMQ010000160         5923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13583_length_5923_cov_4.731254, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000160 DAOSMQ010000000
VERSION     DAOSMQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13583_length_5923_cov_4.731254"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..467
                     /locus_tag="PK829_06790"
     CDS             84..467
                     /locus_tag="PK829_06790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOU40958.1"
                     /translation="MSTPIFFDQVYEVIRRIPRGRVTTYGRVAAMLGRPNAARAVGYA
                     LRASGRRRRGETRAADLPWYRVINSAGRISLPNMHGGADRQAELLRAEGVVVSDDLLI
                     DLSVYLWEGLHPIEIDDILSGRGEP"
     gene            585..1631
                     /locus_tag="PK829_06795"
     CDS             585..1631
                     /locus_tag="PK829_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU40959.1"
                     /translation="MRTPPSRATIPGVVKLIIQIPCYNEAETLPLALAGLPRAIPGID
                     CIETLIIDDGSSDDTAAVAAAHGVSPSHIIRHPQNLGLARAFQSGLDACLRLGADVIV
                     HTDADNQYPGHYIPDLVAPVLAGQAGMVIGDRQVATIDHFSPLKKRMLALGSWMVRNV
                     SGTAVPDAVSGFRAYSREAALRFTILTRYSYTLETVIQAGKLGLGVVSIPITTNPPTR
                     PSRLQRSMWHFIKAQAGTILRLYAFYEPLRTFSYLAAPFLLVGVALWVRFFVNTLLGE
                     SGVGRFIQSLTLGTGLLMVGALIILFGVQADISGKHRLLTQEVLYRLKKMELAEAGER
                     KPLTADRRPPTADR"
     gene            1670..2071
                     /locus_tag="PK829_06800"
     CDS             1670..2071
                     /locus_tag="PK829_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40960.1"
                     /translation="MRNVIALLLFIAALFNLTIFLPGEEAWPLQPFAVHATSWLGFGL
                     ADAPGGTLARVLAVVAALLLLAAALSLVAVLVPARLFRSLALAGALGTLALFLLFLNP
                     NSLLPIVLSLIILYAILSRTWTIGSLSEDDH"
     gene            2071..2655
                     /locus_tag="PK829_06805"
     CDS             2071..2655
                     /locus_tag="PK829_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40961.1"
                     /translation="MRTQSHTAGVVRAGITGIILLASLLAAGCAGGRMDADMITVTLD
                     KPAEDTATVSTADGRAVIDVTSPSGIGGLTATLGKGEWPGEVIVRLRLRGLERLEFGY
                     SNFLLSTGVSSTGDPNPPLMLTVVDEEGNVERASPSANIYYPTIRFVDDGGQALATPA
                     FPLPEGTVIEVVMPPHFHEQEQTSFWMQWIDFYR"
     gene            2665..2970
                     /locus_tag="PK829_06810"
     CDS             2665..2970
                     /locus_tag="PK829_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40962.1"
                     /translation="MYLLMLVLDDTSHLTSTLEAWQRAGVHGATALESTGLQRLMDGR
                     AGDEKAAAGHKTIFAVVNDLEMAELAMRHTQGVIGDLNAPNTGIAFLVPVLAAWGLP"
     gene            complement(2971..3774)
                     /locus_tag="PK829_06815"
     CDS             complement(2971..3774)
                     /locus_tag="PK829_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40963.1"
                     /translation="MYTRRDALRRAAVLAALLALALAASIAAADDDANRNHRAFMPLT
                     AGKPVAPPLDCNVPGSFDALSISGEPLTVDPETHPNLNLSVCGYAQVNAPLRLVELGP
                     VHDPRAPQLPGMFGDWRTPTLTHTYQRYRWDENRNCLNDYSSRWEATVLGMGVSRGET
                     IYTPDSSYDVGGNYEYLVLYAGETDLTLHVGREDEFFGYVIHIDGVCTDPDLLALYRQ
                     LHAAGRNNLPALRGHQAFGVAVGNEIQVAVRDTGSFMDPRSRNDWWQGR"
     gene            3995..4624
                     /locus_tag="PK829_06820"
     CDS             3995..4624
                     /locus_tag="PK829_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU40964.1"
                     /translation="MKQMLIDDERLARQIDFLIEIDRLKHVLRRAYLPGTDRNENTAE
                     HSWHLAVAVLVLAEYAAEPVDLARVVRMALIHDIVEIDAGDTFFFDPVGALNKAEREQ
                     RAAERLFGLLPPDQATGFRAAWDEFEERHSPEARFASALDRFMPLIHNFLTQGRAWRQ
                     NGVRPEQVLAMTAHIDPGAPAIAAAARALVAQAQAAGYFDAVGETAHAS"
     gene            4614..5639
                     /gene="trpD"
                     /locus_tag="PK829_06825"
     CDS             4614..5639
                     /gene="trpD"
                     /locus_tag="PK829_06825"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOU40965.1"
                     /translation="MPLKHAIAQVINGRDLSFEEAEAAMNVIMDGDATPAQIGSYLTA
                     LRMKGETVDEIAGSAHSMRRHVVAVAAGPGNGMLVDTCGTGGDGKHTFNISTTAAFVV
                     AGAGVRVAKHGNRAASSRSGSADVLLALGGNLDLTAPQVGECIDDVGIGFLYALHHHP
                     AMRYAIGPRREIGQRTIFNLLGPLTNPAGATHQLMGVYDPALTAMLAEVLHTLGSRAA
                     YVVHGADGLDELSTTGLNRVSALRDGAVTTFDFDPATLGLPRARLGDLLGGTPEENAA
                     TTRAILAGDDHGPRRDIVLLNAAAALSLETGDWAGGLAAAAAAIDMGAALATLENWIG
                     MTNSFRA"
     gene            5647..>5923
                     /locus_tag="PK829_06830"
     CDS             5647..>5923
                     /locus_tag="PK829_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40966.1"
                     /translation="MSLAAPIYLRLTPPPFDGAYLCIREHQPRRLPARADGPLVVVTF
                     HPGFIAGSYGEWDYFERLSPLLFAAAGAAFRLRVFTVNHPGYDVPSRA"
BASE COUNT          932 a         2086 c         1923 g          982 t
ORIGIN      
        1 catcctgcgc ccgatatgga gattattttg ttaatgtcct aacaggctgt gctaccggct
       61 actgcctacg gtatactgtc gccatgtcca cgcccatctt ctttgaccag gtctacgaag
      121 tcatccgccg catcccccgc gggcgcgtga cgacctacgg ccgcgtggcg gcgatgctcg
      181 gccggcccaa cgccgcccgc gccgtgggct acgcgctgcg ggcttccggc cggcggcggc
      241 gcggcgagac gcgcgcggcc gatctgccct ggtatcgggt catcaacagc gccggccgca
      301 tcagcctgcc caacatgcac ggcggggctg accggcaggc cgagctgctg cgcgccgagg
      361 gcgtcgtcgt cagcgatgac ctgctcatcg acctgagcgt ctatctctgg gaggggctgc
      421 accccatcga gatcgacgac atcctctccg gccgcggcga accgtagtgg ggcagtgggc
      481 agtgggtcag tatccagtag gtcagtgatc agtaggacag ttatccgcgg tctgtagtcc
      541 tctgtagtcc gtggtcggcg gtcggcggtc ggccccgtct ccccatgcgc acgccgccaa
      601 gccgcgctac aatccccggc gtggtcaagc tgatcatcca gattccctgc tacaacgagg
      661 ccgagacgct gccgctggcg ctggccgggc tgccgcgggc tatccccggc atcgactgca
      721 tcgagacgct gatcatcgac gacggcagca gcgacgacac cgccgccgtc gccgcggccc
      781 acggcgtcag ccccagccac atcatccgcc acccgcagaa cctgggcctg gcccgcgcct
      841 tccagagcgg tctcgacgct tgcctgcgcc tgggggcgga tgtcatcgtc cataccgacg
      901 ccgacaacca gtatcccggc cactacattc ccgacctcgt cgcgccggtg ctggccgggc
      961 aggccggcat ggtcatcggc gaccggcaag tggccaccat cgaccacttc tcgccgctca
     1021 aaaagcgcat gctggccctg ggtagctgga tggtgcgcaa cgtcagcggc acggccgtgc
     1081 ccgacgccgt cagcggcttt cgcgcctact cacgcgaggc ggcgttgcgc ttcaccatcc
     1141 tgacgcgcta cagctacaca ctggagacgg tcatccaggc cggcaagctg ggcctgggcg
     1201 tggttagcat ccccatcacc accaacccgc cgacgcggcc gtcgcgcctg caacgcagca
     1261 tgtggcactt catcaaggcc caggcgggca caatcctgcg cctctacgcc ttctatgagc
     1321 cgctgcggac gttcagctac ctggccgcgc cctttctgct ggtcggcgtg gcgctgtggg
     1381 tgcgcttctt cgtcaatacc ctgctgggcg agagtggcgt cggccgtttc atccaatcgc
     1441 tgacactggg cacggggctg ctcatggtcg gcgcgctcat catcctattc ggcgttcagg
     1501 ccgatattag cggcaagcac cgcctgctga cgcaggaggt actgtatcgg ctgaagaaga
     1561 tggaactggc cgaagcgggc gagagaaaac cgctgacggc cgaccgccga ccgccgaccg
     1621 ccgaccgctg acctattgcc ctactgccca ctgcccccca gaggaaccca tgcgcaacgt
     1681 catcgccctc ctgcttttca tcgctgccct gtttaacctg accatcttcc tgcccggcga
     1741 ggaggcctgg ccgctgcaac cttttgccgt ccacgccacg tcctggctgg gctttgggct
     1801 ggccgacgcg ccggggggca cgctggcgcg cgtcctggcc gtcgtggccg cgctgctgct
     1861 gttggccgcc gcgctgagcc tggtcgccgt tcttgtcccg gcgcggctct tccggtcgct
     1921 ggccctggcg ggagcgctgg gcacgctggc tctctttctg ctcttcctca acccgaactc
     1981 actgctgccc attgtgctga gtctgatcat cctctatgcc atcctgagcc gcacctggac
     2041 gatcggcagc ctcagcgagg acgatcacta atgcgcacgc aatcccatac ggccggcgtg
     2101 gttcgcgccg gcatcaccgg cattatcctg ctggccagtc ttctggcagc cggctgcgcg
     2161 ggtgggagaa tggacgccga tatgattacg gtgacgcttg acaaacccgc tgaggacacg
     2221 gccactgtca gcaccgctga cggccgcgcc gtcatcgacg taaccagccc gtcgggcatc
     2281 ggtgggctaa cggccacgct gggcaaaggc gagtggccgg gagaggtcat cgtccggctg
     2341 cggctgcgcg ggctggaacg gctggagttc ggctacagca atttcctgct gtccaccggc
     2401 gtcagcagca ccggcgaccc caacccaccg ttgatgctaa ccgtggtgga tgaggagggc
     2461 aacgtggagc gcgcctcgcc ttcggccaac atctactatc ctaccatccg tttcgtcgac
     2521 gacggcggcc aggcgctggc gacccccgcc ttccccctgc cggagggcac cgttatcgag
     2581 gtcgtcatgc cgccgcactt ccacgagcag gagcagactt cattctggat gcagtggatc
     2641 gatttttatc gctagaggac accgatgtac ttgttgatgc tggttctgga cgacacaagc
     2701 cacctgacaa gcacgctgga ggcgtggcag cgcgccggcg tacatggagc caccgccctg
     2761 gagagcaccg gtctgcaacg gctgatggac ggccgtgcgg gcgacgaaaa agccgccgcc
     2821 ggccacaaga ccatcttcgc tgtggtcaac gacctggaga tggccgagtt ggccatgcgc
     2881 cacacacagg gcgtcatcgg cgacctgaac gcgcccaaca cgggcatcgc ctttcttgtg
     2941 cccgtgttgg ccgcgtgggg attgccctga tcagcgcccc tgccaccagt cattgcgcga
     3001 gcgcgggtcc ataaagctgc ccgtgtcgcg cacggccacc tgaatctcat tgcccaccgc
     3061 cacgccgaac gcctggtggc cgcgcagcgc gggcaggttg ttgcggccgg cggcgtggag
     3121 ctgccggtag agcgccaaca ggtcggggtc cgtgcagacg ccatcgatgt gaatgacgta
     3181 gccgaagaac tcgtcctcgc ggccgacgtg aagcgtcagg tcggtctccc cggcatagag
     3241 caccaaatac tcgtagttgc cgccgacgtc gtagctggaa tcgggcgtgt agatcgtctc
     3301 gccgcggctg acgcccatgc ccagcaccgt cgcctcccag cgcgagctgt agtcgttcag
     3361 gcagttgcgg ttctcgtccc accggtagcg ctggtaggtg tgggtgaggg taggggtgcg
     3421 ccagtcgccg aacataccgg ggagttgcgg ggcgcgcggg tcgtgaacgg ggccaagctc
     3481 gaccaggcgc aggggcgcgt tgacctgggc gtagccgcag acgctcaggt tcaggttggg
     3541 gtgggtctca gggtcaacgg tcagcggctc gccgctgatg ctcagcgcgt caaagctgcc
     3601 gggcacgttg cagtccaggg ggggcgcgac gggcttgccg gcggtcaagg gcatgaacgc
     3661 gcggtggttg cggttcgcgt cgtcatcggc cgcggcgatg ctggcggcga gcgccagggc
     3721 cagcagcgcc gccaggacgg ccgcgcgacg aagagcatct cgacgtgtgt acatgagagg
     3781 tttacctccg tgggcggtta gtgcgtcgca ctttcttgag tgcgtcgcac tttcttgtga
     3841 catctgcgta agaaagggcc tggcactgga gcgcactttc ttgtgacacc ggcgtaagaa
     3901 agggccaggc actgcctcgt cttgatcgga cgggtctgac ttgagccaac aacgcgccgg
     3961 agtataatcc atcgacccgc gaggtaaaac gactatgaag cagatgctca ttgacgacga
     4021 acggctggcg cgacagatcg atttcctaat cgagatcgac cggctgaaac acgtcctgcg
     4081 gcgggcctac ctgccgggca cggatcgtaa cgagaacacg gccgagcata gctggcatct
     4141 ggccgtagcc gtcctggtac tggctgaata tgccgccgag ccagtcgacc tggcccgcgt
     4201 agtgcgcatg gctctcatcc acgacatcgt tgagatcgac gccggcgaca ccttcttctt
     4261 cgaccccgtc ggcgcgctga acaaggccga gcgcgagcaa cgcgccgccg agcgcctctt
     4321 tggcctgctg ccgcccgacc aggcgaccgg cttccgcgcc gcctgggacg agttcgaaga
     4381 gcgccattcg cccgaagccc ggttcgcctc agctttggat cgcttcatgc ccctgattca
     4441 caacttcctg acccagggcc gcgcctggcg gcagaacggc gtccgcccag agcaggtact
     4501 ggccatgacg gcgcacatcg accccggcgc gccggccatc gccgccgcgg cccgcgccct
     4561 ggtggcccag gcgcaggccg ccggctactt cgacgcggtg ggagagacag cccatgcctc
     4621 ttaagcacgc cattgcccag gtcatcaacg gccgcgacct gtccttcgag gaggccgagg
     4681 cggccatgaa cgtcatcatg gacggcgacg ccacgccggc ccagatcggc agctatctaa
     4741 ccgccctgcg catgaagggc gagacagtcg atgagatcgc cggcagcgcc cactccatgc
     4801 gccgccacgt tgtcgccgtg gccgccgggc cgggcaacgg catgctggtg gatacctgtg
     4861 gcacgggcgg cgacggcaag cacaccttca acatctccac cacggccgcc ttcgtcgtcg
     4921 ccggggcagg cgtgcgcgtg gccaagcacg gcaaccgtgc tgcctccagc cgcagcggct
     4981 cggccgacgt gctgctggcc ctgggcggca acctcgacct gaccgcgccg caggtgggcg
     5041 agtgcatcga cgacgtgggc attggcttcc tctatgccct ccaccaccac ccggccatgc
     5101 gctacgccat cggcccgcgg cgcgagatcg gccagcgcac catcttcaac ctgctcggcc
     5161 cgctgaccaa cccggccggg gcgacccacc agctcatggg cgtctatgac cccgcgttga
     5221 cggccatgct ggccgaggtg ctgcacacgc tcggcagccg cgcggcctac gtcgtgcacg
     5281 gggccgacgg gctggacgag ctatcgacca ccggcctcaa ccgcgtcagc gccctgcgcg
     5341 acggcgcagt gacgaccttc gacttcgacc cggcgacgct gggcctgccc cgcgcccggc
     5401 tgggcgacct gctgggtggc acgccggagg agaacgcggc cacgacgcgc gccattctgg
     5461 ccggcgacga ccacggcccg cgccgcgaca tcgtcctgct caacgccgcc gcggcgctga
     5521 gcctggagac gggcgactgg gccggcgggc tggccgcggc ggcggcggcc atcgacatgg
     5581 gcgcggccct ggcgacgctg gagaactgga tcggcatgac caacagcttc cgcgcctagc
     5641 cctgccatgt ccctggccgc gcccatctat ctgcgcctga cgccgccgcc gttcgacggg
     5701 gcctatctgt gcatccgcga gcatcagccg cgccgcctgc cggcccgggc cgacgggccg
     5761 ctggtagtgg tcactttcca tcccggcttc atcgccggct cctacggcga atgggactac
     5821 ttcgagcgcc tgtcgccact gctcttcgcc gccgccggcg cggcgttccg gctgcgcgtc
     5881 ttcaccgtca accaccccgg ctacgacgtg ccctcccgcg ccg
//
LOCUS       DAOSMQ010000161         5909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13627_length_5909_cov_4.423642, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000161 DAOSMQ010000000
VERSION     DAOSMQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13627_length_5909_cov_4.423642"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PK829_06835"
     CDS             <1..1095
                     /locus_tag="PK829_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU40967.1"
                     /translation="AQLRLESAQRAATSAAERHSTILVTEPNAFLSAEDLAEMEFSAR
                     VAAQQANADLAAAQQAYDDLVNGNPGSVSAARAAVAASAAQRDTAQAQLDALREGPSA
                     AEVAAARASLAQAQQQLEQLQSGPSDAQRVAAEVAADSARIDLARAERQLAEATLTAP
                     FDGVVTAVNARAGETASGVLVEMADLSSLQLVLEVDEVDIARVQVGQTAELTPAAWSD
                     ATIPATVAAIAPEASDASELVTYRVYLDVGESDLPLRVGMTADARLLAEQLTGVLLLP
                     NRAIQADRATGTYSVNRVTVAADGTETIDSVPVTIGLRDNRYTQITGGLAAGDVVQIG
                     NALPVQTFGPAGGDEGNQEGPSFIPPAPGD"
     gene            1098..1910
                     /locus_tag="PK829_06840"
     CDS             1098..1910
                     /locus_tag="PK829_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU40968.1"
                     /translation="MITMRSITKTYQMGENVVHALRGLDLDVSEGEFLAIMGPSGSGK
                     STLMNILGCLDTPDSGTYTLGGEDVSQLSDDEQARIRGRRVGFVFQQFNLLPRTPALK
                     QVALPLMYAGLSRAERMEKARAALTRVGLDDRADHRPDELSGGQQQRVAIARALVNEP
                     NIILADEPTGALDTHTGEEILALFEELNRQGITVIVITHDPDVAARAGRTVWIRDGLV
                     VDHGHVGRLGNPPLPAEDGRVSRAITEESGMPSRPATEEGGMPSRPTSEGAA"
     gene            1910..3127
                     /locus_tag="PK829_06845"
     CDS             1910..3127
                     /locus_tag="PK829_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU40969.1"
                     /translation="MNLSESLLTALDSLRSNKLRAALTMLGVIIGVAAVIALLSIGNG
                     VSASINDQINAIGTNLILISTDRDNSDGYPTLTLSDVAALADPLRAPDVADVAASVGG
                     SFAVTAGGNSSRSSISGVTANYFPVNNIDDFAAGDVLTENDIDTQARVAVLGWQVAED
                     LFPDSYAVGQSVKINGASYEVVGVLAQSGSAFGGNDTSVFVPISTAQSRLVTNRTRGG
                     QKAVDSIIAQAASNERNEAALEQIAAVLREEHRIAYADDDDFQLLSQSDLLDTAGQIT
                     GVLTAFLGAIAGISLLVGGIGIMNIMLVSVTERTREIGIRKAVGALRRDILLQFLLES
                     VVLSLIGGLTGIALGWLIAAVAGRAIDLSVTLQLGTVLLATGFATAVGLVFGIYPAWR
                     AARLRPIEALRYE"
     gene            3275..3967
                     /locus_tag="PK829_06850"
     CDS             3275..3967
                     /locus_tag="PK829_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU40970.1"
                     /translation="MRRILVVDDDREVVRLIRAYLEQAGFEVLMAYDGDTAVHVIRRD
                     RPDLLLLDLMLPGKDGWEITRLVRSDPALARTPIIMLTARVDDTDKIIGLELGADDYV
                     TKPYNPREVVARVRARLRQPDVAPPATLRAGRLEMDARRREVRVDGRPVELTPTEFNL
                     LHVLMQQAGYVLTRSELARLALGIDFEGVERTLDSHIRNLRQKIEPDPANPIYVQTVY
                     GVGYRLAEEDAL"
     gene            3964..5061
                     /locus_tag="PK829_06855"
     CDS             3964..5061
                     /locus_tag="PK829_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU40971.1"
                     /translation="MNRLWVRFALIISGIMLLVVFLPMFIGFRFRPSDDLATQLRALL
                     ESLPPDLQAELQLRIQETIGLFISRALLLVVTVAILAGVVLSRLLLAPLNDLATGAAA
                     IARRELATRVPVRGSVETRAVAQSFNHMASELEQAEGLRQQLLADVAHELRNPLHVLR
                     GNLQAIFDGVYPLNQAEVSRLLDQTDQLTRLVNDLHELALAEARQLPLDRQPLNLGLL
                     VKDTAADFQALAAAEGVALRVELLGVPPTVEVDAARIRQMLQNLVVNALRHTPRGGNV
                     LVSVGRAGDTAEVIVRDSGQGIAPEHLPQVFDRFFRTDGARDREHGGAGLGLAIVKAI
                     VEAHGGNVAVASAGVGQGSTFTVTLPIAPSA"
     gene            5442..>5909
                     /locus_tag="PK829_06860"
     CDS             5442..>5909
                     /locus_tag="PK829_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40972.1"
                     /translation="MTTSRRALWVSLAVAVAALLYLSTVQTIINGGGHPYVTDVGEHQ
                     NALPRWGTIHHSGYPQWTALGSLFVSVVRLVGVSPAAAVSLYSLVWGLLTVALLVVLA
                     MELGAAGPFAALGALAAAVATSVWMDASIGELHTATMALTLATLLFALRFGRSG"
BASE COUNT          952 a         2064 c         1911 g          982 t
ORIGIN      
        1 gcccaattgc ggctggagag cgcccaacgc gccgccactt cggccgccga gcggcacagc
       61 accatcctgg tgactgagcc aaacgctttc ctgtcggccg aagacctggc cgagatggaa
      121 ttcagcgccc gcgtcgccgc ccaacaggcc aacgccgatc tggccgcggc ccagcaagcc
      181 tatgacgacc tcgtgaacgg caaccccggc agcgtcagcg ccgcccgggc cgctgtcgct
      241 gcgtcggctg cccagcgcga cacggcccag gcccaactcg atgccctgcg cgaaggcccg
      301 agcgcggccg aggtggccgc ggcccgcgcc tcgctggccc aggcgcaaca gcaactggag
      361 cagctacaga gcggccccag cgacgcccag cgcgtggccg ccgaagtggc cgccgactcg
      421 gcgcgcatcg acctggcgcg ggccgagcgt cagctggccg aagccacatt gaccgcgccc
      481 ttcgacggcg ttgtcaccgc cgtgaacgcc cgcgccggcg agacggccag cggcgtgctg
      541 gtggagatgg ccgacctgag cagcctgcaa ctggtgctgg aggtcgatga ggtcgatatc
      601 gcccgggtgc aggtgggcca gacggccgaa ttgaccccgg ccgcctggag cgacgcgacg
      661 attccggcca cggtggcggc cattgccccg gaggccagcg acgccagcga gcttgtcacc
      721 tatcgcgtct atctggacgt gggcgagagc gatctgccgc tgcgcgtggg catgaccgcc
      781 gacgcccggt tgctggcgga gcagttgacc ggcgtgctgc tgctgcccaa tcgcgccatt
      841 caggccgacc gcgccaccgg cacctacagc gtcaaccgcg tcaccgtcgc cgccgacggg
      901 acggaaacca tcgacagcgt gccggtgacg attggcctcc gcgacaaccg ctacacccag
      961 atcaccggcg gcctggccgc gggcgatgtg gtgcaaatcg gcaacgccct gcccgtccag
     1021 acctttgggc cggcgggcgg ggacgaaggc aaccaggaag ggccgtcctt catcccgccg
     1081 gccccgggag attagctatg atcaccatgc gcagcatcac caagacctac cagatgggcg
     1141 agaacgtcgt ccatgccctg cgcggccttg acctggacgt gagcgagggc gagtttctgg
     1201 ccattatggg gccgtcgggc agcggcaaga gcacgctgat gaacatcctc ggctgcctgg
     1261 acactcccga cagcggcacc tacaccctgg gcggcgaaga tgtcagccag ctgagcgacg
     1321 acgagcaggc ccgcatccgc ggccgccgcg tcggcttcgt cttccagcag ttcaacctgc
     1381 tgccgcgcac gccggccctc aagcaggtcg ccctgccgct gatgtacgcc gggctgagtc
     1441 gcgccgagcg aatggaaaag gcgcgcgcgg cgttgacgcg cgtcggcctg gacgaccgcg
     1501 ccgaccaccg ccccgacgag ctttcgggcg gccagcagca gcgcgtggcc atcgcccgcg
     1561 ccctggtcaa cgaaccgaac atcatcctgg ccgatgaacc gaccggcgca ttggacaccc
     1621 acactggcga ggaaattctg gccctgttcg aggaactcaa ccggcagggc atcaccgtca
     1681 tcgtcatcac ccacgacccg gacgtggccg cccgcgccgg gcgcaccgtc tggattcgcg
     1741 acgggttggt cgttgaccac ggccatgtag ggcggcttgg taacccgcct cttcccgctg
     1801 aagacgggag ggtgtcgcgc gctataacag aagaaagcgg gatgccatcc cgccctgcga
     1861 cagaagaagg cgggatgcca tcccgcccta caagtgaagg agcggcctaa tgaacctgag
     1921 cgaaagtctg ctgacggccc tcgatagcct gcgctcaaac aagctgcggg cagccctgac
     1981 gatgttgggc gtcatcatcg gcgtggccgc cgtcatcgcc ctgctgtcaa ttggcaacgg
     2041 cgtcagcgcc tcgatcaacg accagatcaa tgccatcggc acgaacctga tcctcattag
     2101 caccgaccgc gacaacagcg acggctaccc gaccctcacg ctgtcggacg tggccgcgct
     2161 ggccgaccca ctgcgcgcgc ccgacgtggc cgacgtagcc gcgtccgtcg gcggcagttt
     2221 cgccgtcacc gccggcggca atagctcccg gtcgagcatc agtggcgtga cggccaatta
     2281 cttccccgtc aacaatatcg acgactttgc cgccggcgat gtcctcaccg agaacgacat
     2341 cgatacccag gcgcgcgtgg ctgtgctggg ctggcaggtg gccgaagacc tctttcccga
     2401 cagctatgcc gtcggccaga gcgtgaagat caatggcgcc agctatgagg tcgtcggcgt
     2461 gctggcccag tcgggcagcg ccttcggcgg caacgatacc tctgtcttcg tgcccatctc
     2521 caccgcccag agccggttgg tgaccaaccg cacgcgcggc ggccagaagg cggtcgattc
     2581 catcatcgcc caggccgcca gcaacgagcg caacgaggcc gcgctggagc agatcgccgc
     2641 cgtgctgcgc gaggagcacc gcatcgccta cgccgacgac gacgacttcc agttgctcag
     2701 ccagtcggac ctgttggaca cggccgggca gatcaccggc gtgctgaccg ccttcctggg
     2761 ggccatcgcc ggcatctccc tgctcgtcgg cggcatcggc atcatgaaca tcatgctcgt
     2821 ctcggtgacc gaacgcacgc gcgagatcgg catccgcaag gccgtcggcg cgctgcggcg
     2881 cgacatcctg ctccagtttc tactggagtc ggtcgtgctc agcctcatcg gcggcctgac
     2941 cggcatcgcc ctgggttggc tcatcgccgc cgtcgccgga cgcgccatcg acctgagcgt
     3001 gacgctgcaa ctgggcacgg tgttgctggc gaccggcttt gccaccgccg tcggtctcgt
     3061 cttcggcatc tatcccgcct ggcgggccgc ccgcctgcgg ccgatcgagg ctttgcggta
     3121 tgagtaagag gctcacgcca aggcggtaag aatgatctca cgcaaagacg cagagtacgc
     3181 aaagctttag aagctttctt cgcttggcgt tcttggcgtc tttgcgtgag attttctttg
     3241 cgccttggcg tgagctctgc ataatggact ggctatgcgc cgtattctcg tcgttgacga
     3301 cgaccgcgaa gtcgtccgtc tgattcgggc ctatctggag caagccggct tcgaggtgct
     3361 gatggcttac gatggcgata cagccgttca cgtcatccgc cgcgaccggc ccgacttgct
     3421 gctcctcgac ctcatgctgc ccggcaagga cggctgggag atcacccgtc tggtgcgcag
     3481 cgacccggcg ctggcccgca cgccgatcat catgctgacc gcgcgcgtgg acgacaccga
     3541 taagatcatc ggcctggaac tgggggccga cgactatgtg accaagccct ataacccccg
     3601 cgaggttgtg gcccgcgtgc gcgcccgcct gcgccaaccc gacgtggccc cgccagcgac
     3661 gttgcgcgcc ggccggctgg agatggacgc gcggcgtcgc gaggtgcgcg tggacggccg
     3721 tccggttgag ttaacgccga ccgaattcaa tctcttgcac gtgctcatgc agcaggccgg
     3781 ctacgttctg acccgcagcg agttggcccg tctggccctg ggcatcgact tcgagggcgt
     3841 cgagcgcacc ctcgatagcc acatccgcaa cctgcgtcag aagatcgaac ccgaccccgc
     3901 caatccgatc tacgtgcaga cggtctacgg cgtcggctac cgcctggctg aagaggacgc
     3961 gctatgaatc ggctgtgggt gcgttttgcc ctgatcatta gcggcatcat gctgctggtt
     4021 gtctttctac cgatgttcat cggctttcgt ttccggccgt ccgacgatct ggccacgcaa
     4081 ctgcgggcgt tgctggagtc gctgccgccc gatttgcagg ccgagctgca actgcgcatc
     4141 caggagacaa ttggcctttt catcagccgg gcactgctgc tcgtcgtcac cgtggcgatt
     4201 ctggccggcg tcgtcctcag tcgcctgttg ttggctccgc tcaacgacct ggccaccggc
     4261 gcggcggcca tcgcccggcg cgaactggcc actcgcgtgc cggtgcgcgg cagcgtcgag
     4321 acacgcgctg tcgcccaatc gttcaaccac atggccagcg aactggagca ggccgaggga
     4381 ctgcggcagc aactgctggc cgacgtggcc cacgaactgc gcaacccgtt acacgtcctg
     4441 cgcggcaact tgcaggccat cttcgacggc gtctacccgc tcaatcaggc cgaggtgtcg
     4501 cgtctgctgg atcagaccga ccagttgacg cggctggtga atgatctgca cgagctggcg
     4561 ttggccgagg cccgccagtt gcccctggat cgccagccgc tgaacctggg actgctggtg
     4621 aaggacacgg ctgccgactt ccaagcattg gccgcggccg agggcgttgc tctgcgtgtc
     4681 gagttgctgg gtgtgccgcc aacggtcgag gttgacgccg cccgcatccg ccagatgctg
     4741 caaaatctgg tcgtcaacgc cctgcgccac acgccccgtg gcggcaatgt gctggtcagc
     4801 gtcggccgcg ccggcgacac ggccgaggtc atcgtccgcg atagcggcca gggcattgct
     4861 ccggagcatc tgccgcaggt gttcgaccgt ttctttcgca ccgacggcgc gcgcgaccgg
     4921 gagcacggcg gcgcgggcct ggggttggcc atcgtcaagg ccattgtgga ggcccacggc
     4981 ggcaatgtgg ccgtggccag cgcgggggtg gggcagggta gtacgttcac tgttaccttg
     5041 cccatcgctc cctccgctta gttccctctc cctgtgggag agggatcggc gttccctctc
     5101 cctgtgggag agggttaggg agaggggcct ggattcctgg cccgacgaga accctcccct
     5161 aacccctccc aaagggagga ggatcggctt ccctctccct gtgggagggg gatcggtgtt
     5221 ccctctccct gtgggagagg gttagggaga ggggcctggt tccctggccc gatgagaacc
     5281 ctcccctaac ccctcccaaa gggaggggga tcggagttcc ataatgcctt gcgcctgacc
     5341 catccaccgg gctacactcc acttcatgac cgtctacaac aacctgcgac cactcgactg
     5401 acccactgac cgactgaata ctgcttactg ctgactgtcc tatgaccacc tcccgccgcg
     5461 ccctctgggt cagcctcgcc gtcgctgtgg ccgcgctgct ctacctctcc accgtccaga
     5521 cgatcatcaa cggcggcggc cacccctacg tgacggacgt gggcgagcac cagaacgccc
     5581 tgccgcgctg gggcaccatc caccacagcg gctacccgca gtggacggcc ctcggctcgc
     5641 tattcgtcag cgtcgtgcgc ctggttggcg tctcgccggc ggcggccgtc tcgctctatt
     5701 cgctcgtctg ggggctgctg acggtggccc tgctggtcgt gctggcaatg gagttgggcg
     5761 cggccgggcc attcgcggcg ctgggggcgc tggccgcggc cgtcgccacc tcggtgtgga
     5821 tggatgcctc tatcggcgaa ctgcacacgg ccacgatggc cctgacgctg gcgacgctgc
     5881 tcttcgccct gcgcttcggc cgctccggc
//
LOCUS       DAOSMQ010000162         5907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13638_length_5907_cov_4.448735, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000162 DAOSMQ010000000
VERSION     DAOSMQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13638_length_5907_cov_4.448735"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3048
                     /gene="pulA"
                     /locus_tag="PK829_06865"
     CDS             <1..3048
                     /gene="pulA"
                     /locus_tag="PK829_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA];
                     GO_process: GO:0005983 - starch catabolic process
                     [Evidence IEA];
                     GO_process: GO:0051678 - pullulan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-type alpha-1,6-glucosidase"
                     /protein_id="HOU40973.1"
                     /translation="ESGAPGGANIPFTVDADGTLVTFLWNVNNKIMTIQVGGSGQVGN
                     LAEARAYWVAADTIVWDVAYSPDNQYAFHYTDTGEPLVLDAEGVSGSPLILSYVPEGL
                     SPELAARFPHLAGQSVFHLPARLGTLRLALKGQVAVAVTDAEDNLLDATSLQIAGALD
                     DIYAYDGPLGVTYEDGVPVLRVWAPTAQRVRLVLFADANPDTPGEATIMRVDPETGVW
                     TLAGEADWDRQYYLYEVTVFAPSTGAVETNLVTDPYSVSLSANSARSQIVNLDDADLK
                     PEGWDARARPPLAAPEDIVIYELHIRDFSAYDQSVPAELRGTYGAFAQGDGAVHLANL
                     AAAGVTHLHLLPSFDMATVNENAAERVEPDVATLAALPPDSDQQQALIGETRDQDAFN
                     WGYDPLHYNVPEGSYSTDPNGVTRILEYRQMVQALNAMGLRVVADVVYNHTNASGQND
                     NSVLDRIVPGYYHRLNATGRVETSTCCQNTATEHTMMRRLMVDSVRLWAEQYGIDAFR
                     FDLMGHHMKDDMLAVRAALDEIDPSIYVYGEGWDFGEVAGNARGVNATQRNMAGTGIG
                     TFNDRLRDAARGGSPFGGQQEQGFINGLFYDPNETDQGTADEQLARLLHLTDLIEVGL
                     AGNLAGYTFVGSSGETVSGADVDYNGNPAGYTADPQENIVYVSKHDNETLFDAIQYKA
                     PATADMAARVRMQNMGNSLVMLSQGVPFFQAGDDLLRSKSLDRNSYNSGDWFNAIDWT
                     GQTTNWGRGLPMAGDNQSMWPVMAPLLANPDLMPQPEHIAAAAAHYQEMAQVRASSPL
                     FRLPTAEAVQARLQFLNTGPEQIPGVIIMSLSDVGSGNLGDNLDPAADRIVVIFNAND
                     EPLSFAAPEWAGREMALHPVLANSADAIVRASAWDADAATFSVPGRTTAVFVQPEGTE
                     PAETTEAIQPTAEPTAEPTAAPTSEPTAAPTAAPTATAEVVTVAPLDTPEAPAATAEV
                     APTPAPAPEDNNSGVTAVALLVIAAAAGAVAGIIAWLRSRREE"
     gene            complement(3183..>3475)
                     /locus_tag="PK829_06870"
     CDS             complement(3183..>3475)
                     /locus_tag="PK829_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015127007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU40974.1"
                     /translation="RAAAGDALAREILRRGAWALGVGIGHAANLINPQRFILGGGVTK
                     SGEAWWAEVRRSAVATALPEVQFDIVPAALGDDAPLWGALALTELSSADGTH"
     assembly_gap    3476..3575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3576..4272)
                     /locus_tag="PK829_06875"
     CDS             complement(<3576..4272)
                     /locus_tag="PK829_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU40975.1"
                     /translation="MTTLILALDFGGTKLTAALFGTEEETHAKTPRRQAQSSELGVFA
                     SWRETSSCSLLDLRRVITPGDADADYEQAAMLALARDLLAGRQPAAVGVSFGGPVDFA
                     AGVVRLSHHVRGWEALPLRDRLAAEFGCPVAVDNDANVAALGEHRYGAGRGLSDLMYV
                     TVSTGVGGGWILGGRPWRGHEGMAGEIGHIVADPAGPPCLCGKRGCVERLASGPYMSA
                     DYVAQAVSLPGSGG"
     gene            complement(4362..5168)
                     /locus_tag="PK829_06880"
     CDS             complement(4362..5168)
                     /locus_tag="PK829_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU40976.1"
                     /translation="MYVVTNYPDGVFNWIDLSTTDIAGARAFYSGLFGWQANNVPVGD
                     SGVDYTFFELDGHAVAGGGQMMPEQQAAGQPAAWTSYVKYDDIDAAVARAGAAGGTVL
                     MPPMEIPNSGRLAVLTDPSGAVFGIWSPKPFPGAALVNMPGTLAWNELQTRDLPAARA
                     FYGQVFGWTASEMSDSYTTFAADGRMQCGSMPIGGEMWDGVPSNWAVYFMVEDVDATA
                     ARVAELGGTVLVGPQAAGEMGRLIVVRDPQGAIFTAMQFNGPMDPPPGFN"
     gene            5612..>5907
                     /locus_tag="PK829_06885"
     CDS             5612..>5907
                     /locus_tag="PK829_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOU40977.1"
                     /translation="MAQEMDVYVQDLHARRATAAGTEKDAAAQHGKGRLTARERIDLL
                     FDPGTFTEIDTLVLPRHETYPGGKGSRLGDGVITGFGLINGRRAFASSQDAAVMG"
BASE COUNT         1048 a         2045 c         1846 g          868 t
ORIGIN      
        1 gagagcggcg cgcccggtgg agccaacatc cccttcaccg tcgatgccga cggtacactg
       61 gtgaccttct tgtggaacgt caataataag atcatgacta tccaggtcgg cggcagcggc
      121 caggtgggca acctggccga ggccagggct tattgggtcg ccgccgatac cattgtctgg
      181 gatgtggcgt acagccccga caatcaatac gccttccact acaccgacac gggcgagccg
      241 ctggtgctcg acgcggaggg cgtcagcggc tcgccgctca ttctgagcta cgtgcccgaa
      301 gggctgtcgc cggagctggc ggcccgcttc ccccacctgg ccggccagtc ggtcttccat
      361 ctgccggcgc ggctgggcac gctgcggctg gcgctgaagg ggcaggtggc cgtggccgtg
      421 accgacgccg aggacaacct gctcgacgcc accagcctgc aaatcgccgg cgcgctcgat
      481 gacatctacg cctatgacgg cccgttgggt gtcacctacg aggacggcgt gcctgtcttg
      541 cgcgtttggg cccccacagc ccaacgcgtg cgtctcgtcc tctttgccga cgccaacccc
      601 gacacgcccg gcgaggcaac gatcatgcgc gtcgacccgg aaaccggcgt ctggacgctg
      661 gccggcgagg cggactggga tcgccagtac tatctctacg aggtgaccgt ctttgccccc
      721 agcaccggcg cggtggagac gaacctcgtc accgacccct actcggtcag cctgtcggcc
      781 aacagcgccc gcagtcagat cgtcaacctc gacgacgccg acctgaagcc ggagggctgg
      841 gacgcgcgcg cgcggccgcc cctggccgcg ccggaggaca tcgtcatcta cgaactccac
      901 atccgcgact tcagcgccta tgaccagagc gtgccggcgg agttgcgcgg cacgtacggc
      961 gcgttcgccc agggggacgg cgcggtgcat ctggccaacc tggccgcggc tggcgtgacc
     1021 caccttcacc tgctgcccag cttcgatatg gccaccgtca acgagaacgc cgccgagcgc
     1081 gttgagccag acgtggccac gctggccgcg ctgccgcccg acagcgacca gcagcaggcg
     1141 ctcattggcg aaacgcgcga ccaggatgct ttcaactggg gctatgatcc gctgcactac
     1201 aacgtccccg aaggcagcta cagcaccgac cccaacggcg tgacccgcat cctggagtat
     1261 cgccagatgg tgcaggccct caacgcgatg gggctgcgcg tcgtcgccga cgtagtctat
     1321 aaccacacca acgccagcgg ccagaacgac aactcggtcc tcgaccgcat cgtacccggc
     1381 tactatcacc gcctgaacgc caccggccgc gtcgagacga gcacctgctg ccagaacacg
     1441 gccaccgagc acaccatgat gcgccggctg atggtcgatt cggtgcgcct gtgggccgag
     1501 cagtacggca tcgacgcctt ccgcttcgac ctgatgggcc accatatgaa ggacgacatg
     1561 ctggccgtgc gcgcggcgct ggacgagatc gacccgtcaa tctacgtcta cggcgagggt
     1621 tgggacttcg gcgaggtagc cggcaacgcc cgcggcgtca acgcgacgca gcgcaacatg
     1681 gccggcacgg gcatcggcac gttcaacgac cggctgcgcg acgctgcccg cggcggcagc
     1741 cccttcggcg gccagcaaga gcagggcttc atcaacggcc tgttctacga tcccaacgag
     1801 accgaccagg gcacggccga cgagcaactg gctcgcctgt tgcacctgac cgacctcatc
     1861 gaggtcggcc tggccggcaa cctggccggc tataccttcg ttggctcgtc gggcgagacg
     1921 gtcagcggcg cggacgtgga ctacaacggc aaccccgccg gctacaccgc cgacccgcag
     1981 gagaacatcg tctacgtgtc taagcacgac aacgagacgc tcttcgacgc catccagtat
     2041 aaggccccgg ccacggccga catggccgcc cgcgtgcgca tgcagaacat gggcaatagc
     2101 ctggtcatgc tcagccaggg cgtgcccttc ttccaggccg gcgatgactt gctgcgctcc
     2161 aagtcgctcg accgcaacag ctacaactcc ggcgactggt tcaacgccat cgactggacg
     2221 ggccagacga ccaactgggg gcgcggtctg ccgatggccg gcgacaacca gagcatgtgg
     2281 ccggtgatgg ccccgctact ggccaacccc gacctgatgc cgcagccgga gcacatcgcc
     2341 gccgccgcgg cccactacca ggagatggcc caggtgcgag ccagttcgcc gttgttccgg
     2401 ctgccgacgg ccgaggccgt gcaggcccgc ctgcaattcc tcaacaccgg cccggagcaa
     2461 atccccggcg tgatcattat gagcctgtcg gacgtgggga gtggaaacct cggcgacaac
     2521 ctcgacccgg ccgccgaccg cattgtcgtc atcttcaacg ccaacgacga gccgctgtcg
     2581 ttcgccgcgc cggagtgggc cgggcgggag atggccctgc acccggtgct ggccaactcg
     2641 gccgacgcca ttgtgcgcgc gtcggcctgg gacgcggacg cggccacatt cagcgtgccc
     2701 ggccggacga cggccgtctt cgtccagcca gagggcacgg agccggccga aacgaccgag
     2761 gcgatccagc ccacggcgga gccaaccgca gagccgacgg ccgcgcccac cagcgaaccg
     2821 acggcggcgc caaccgccgc gccgaccgcg acggccgagg tcgtcaccgt ggctccgctg
     2881 gacacgccag aagcgcccgc agccacggcc gaggttgccc cgacgcccgc gcccgcgccg
     2941 gaagacaaca actccggcgt caccgccgta gccctgctgg tgatcgccgc ggcggccggg
     3001 gccgtggccg gcatcattgc ctggttgcgc agccgccggg aagagtaggc ggattaagag
     3061 gctcacgcga aggcgccaag gagcgaaggt gcaaagaaga aaaagaagag tgcccagcat
     3121 tgcgttgtag tgcctgcttc ctttgcgcct ttgctttcct ggcggctttg cgtgagctag
     3181 tcttaatgcg ttccatctgc ggaagaaagc tccgtcagag ccagcgcgcc ccacagcggc
     3241 gcgtcgtcgc ccagggcggc ggggacgatg tcaaactgca cttccggcag agccgtggcc
     3301 acggccgagc gccgcacctc ggcccaccac gcctcgcctg acttcgtcac cccgccgccg
     3361 aggatgaagc gctgcgggtt gatcaggttg gcggcatggc caatgcccac gcccagcgcc
     3421 cacgcgccgc gccggagtat ctccctcgcc agcgcgtctc ccgccgccgc ccgccnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctccc ccgctccccg gcaggctgac
     3601 agcctgcgct acatagtcgg cggacatgta ggggccagac gccaaccgct ccacacaccc
     3661 ccgtttgcca cacagacacg gcggcccggc cggatcggct acgatgtggc cgatctcgcc
     3721 ggccatgccc tcgtgcccgc gccacggccg cccacccaga atccagccgc cgccaacgcc
     3781 tgtgctgacg gtgacataca tcaaatcgct caggccgcgg cccgcgccat agcgatgctc
     3841 gcccagcgcg gcgacgttgg cgtcgttgtc cacagccacg gggcagccga actcggccgc
     3901 cagccggtcg cgcagcggca gcgcctccca gccgcggacg tggtgggaca ggcggacgac
     3961 gccagcggcg aaatcgaccg ggccgccgaa gctgacgcca acggccgccg gctgccgccc
     4021 ggccagcaag tcgcgggcca gggccagcat ggctgcctgt tcatagtccg cgtcggcgtc
     4081 gccgggggtg atcactcgcc gtaggtcaag gagagagcag gaagaagtct cacgccaaga
     4141 cgcgaagacg ccaagctcag aactctgagc ttggcgtctt ggcgtcttgg cgtgagtctc
     4201 ttcttctgta ccgaagagcg ccgcggtgag cttggtgccg ccgaagtcga gagccagaat
     4261 gagcgtcgtc atgcccacat tctagcccca acccccctgt ctacgaagaa cgcgcatcac
     4321 cggatgcgcg ctcttcattc ctaattcgtc attcgcaatt cctaattgaa ccccggcggc
     4381 gggtccatcg gcccgttgaa ttgcatggcg gtgaagatcg cgccctgagg atcgcgaacg
     4441 acgatcaagc gccccatctc gcccgccgcc tgggggccga cgagcaccgt gccgcccaat
     4501 tcggccacgc gggccgcggt ggcgtccacg tcttcgacca tgaagtatac ggcccagttg
     4561 gacggcaccc cgtcccacat ctcgccgccg atgggcatag agccgcattg catgcggccg
     4621 tccgcggcga aggtcgtgta gctgtcggac atctcgctgg ctgtccagcc aaagacctgc
     4681 ccatagaagg cgcgcgccgc cggcaggtcg cgggtttgca gctcgttcca ggccagggta
     4741 ccgggcatat tgaccagcgc cgcgccgggg aagggttttg ggctccagat gccgaagacc
     4801 gcgccgctgg ggtcggtcag cacagccaga cggccgctgt tgggaatctc catcggcggc
     4861 atgagcaccg tgccgccggc cgctccggca cgggccacgg ccgcgtcaat gtcgtcatac
     4921 ttgacgtagg atgtccaggc cgcgggttgg ccggcggcct gttgctccgg catcatctgc
     4981 ccgccaccgg cgaccgcatg gccatcaagc tcgaagaagg tatagtcgac gccgctgtcg
     5041 ccaacgggta cgttgttggc ctgccaaccg aacaggccac tgtagaaggc tctggctccg
     5101 gcaatgtcag tcgtcgatag gtcgatccag ttgaagacac cgtcaggata attagtcacc
     5161 acatacatgt ctggcctccg gttgttaaag gctcccatac gtagcgaaca ggtcgtcgcc
     5221 actgagaagt ataccacaga atcgaacgtc tggtctagtg ccagggtcag ggcctttcca
     5281 aagtcgtcta gcgaccattg acctgacagg ggggcggcca cgaatggtac ttccaccaga
     5341 ggacgaccgg ccgcccacct gtcatgtcgg tgcgcctggg gtgactttgg caaagcccta
     5401 gtgccagtgt agccgccagc gcctgcggac atgacacttc gcgccaaaac aggtgacgga
     5461 tgacactaaa tgttaccgcg atggcatggc acaatagtcg ccatgggcgc aatggggctt
     5521 atcgcttgct ggcccacatc cgccaattgg gggagccaaa acaaaaccac gttgccacgg
     5581 ccacccggca gcgatggatc gggagaatgg aatggcacaa gagatggatg tttacgtcca
     5641 ggatttgcac gcccggcggg cgacggccgc cggcaccgag aaagacgctg ccgcgcagca
     5701 tggtaagggt cgtctgacgg cgcgtgagcg catcgacctg ctctttgacc ccggcacgtt
     5761 caccgaaatc gacaccctcg tcctgccccg ccacgagacc tatcccggtg gcaaaggctc
     5821 acgcctgggt gacggcgtca tcaccggttt tgggctgatc aacggccggc gcgccttcgc
     5881 ctcgtcgcaa gacgccgccg tcatggg
//
LOCUS       DAOSMQ010000163         5890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13687_length_5890_cov_4.190060, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000163 DAOSMQ010000000
VERSION     DAOSMQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13687_length_5890_cov_4.190060"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PK829_06890"
     CDS             <1..757
                     /locus_tag="PK829_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU40978.1"
                     /translation="DGATYAAAGCAVYDWTSGGLNSGGETITFRNRFGVPVLAFAYGT
                     AAPWPTEPKGGGPSLALLDLTYDNSLPTSWAASRETGGTPCAENFPPLPPVAAIVINE
                     IHYNPDDAAQGPDADYEFIELVNTSGEPVALEGYALTGVDYTFAAGASIAAGEHIVIA
                     ASAATYAGSYQVFQWDAGSDLADDGETLTLTDGYGRTVDEVTYGDAGDWPAAPDGAGP
                     SLALRNPGDDNSLAANWTASNETGGTPGAANGL"
     gene            827..2995
                     /locus_tag="PK829_06895"
     CDS             827..2995
                     /locus_tag="PK829_06895"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU40979.1"
                     /translation="MFIAVLAALLAGLALAARLRADAPPVVIAEVLAGNASTNLDPDY
                     KNFVPWVELRNDGATAVNLTGYRLSNDLAVPNRWTLPAGVSIPAGGALLLWLDGQATG
                     RHAPFVLDMSGELGLFAPDGTLIDSLTFGPQRPDVSYGRAPGGDWLTFDPPTPGAANS
                     GGWADPTPAGPPTLSPPGGFYAGAQAVTLAAAPGATIRYTTDGRRPTAASPLYSGPIA
                     VNAPTAIRARAFPAGGLPSETATATYLVGVSPNVAVVSLATDPAYFFDDFIGIYVDGR
                     AGIANPDCGPKVANWNRPWERPVSVEIYDAGGALLLRQDGGVAIAGSCTRKDPQKQLQ
                     LFARPGYGDDDFSVALFADKPAAAYGRIILRPGGQDVANTLLRDALGQQLVAGRMDID
                     RQAYRPGVVFINGAFWGIYGVRERMDEYYVTSTYGLGLGEFDLLEKNAHVMVGSRAGW
                     DALYAYLRDNNPATPSVYATVQSRIDIDEYINYQIAEIYADNIDWPHNNIRFWSAHAG
                     GRWRWMLYDMDAAFGRTTKGYNNNTFKFAAAKTGKRAYNALVLRRLLLSPEFKAEFGQ
                     RFMAHLNTTYAPARVRGIIDDMAAAIAPQMPAQVARWGKPRSVAFWQSEVGRLRVFAD
                     QRPGYLTGFLNTFLGAPGTAALTVNHNAAQGRVLVAGVDVPGNYSGPHFRTLPVRLEA
                     VPAAGYVFVQWAETGETNAAVAVVLSGAAATRTAVFAPAP"
     gene            3033..5717
                     /locus_tag="PK829_06900"
     CDS             3033..5717
                     /locus_tag="PK829_06900"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU40980.1"
                     /translation="MNRHRLSLMFLLGALLLGLVAAPRLRAAAPPIVINEILAGNASV
                     NLDPQFTNFAPWVELYNAGSSAVSLAGYHLSDDATNPAGYTLPASASIPAGGRLLLWY
                     DEMATGVHTSLSLDMRGDTVALFMPDGTLVDAVTYGSQLDGETLVDVSYGRAGDGAGA
                     WASFDTPTPGAANSGGLATAARLARPAFSPPGGVYAAGQSVTLTAEPGAQIRYTTDGR
                     KPTAASPLYTGPITVNTPAVIRARAFAAGALSSRPATASYLIGVDSDLAVVSLATDPA
                     NLFDPQIGIYVVGKNGAKGRCGPVANWNRAWERPVSLELWEPGGAFAFQQDVGFEIQG
                     DCTRAMPQKSLEIKARRRYGDNDIDYAVFPGNPLDEYERLVLRGGGNHNAYLSMFREP
                     MVHRLHEQTMALDQQQYRPTAVFINGVYWGIHNLRDKADEALIEQNYGLDADDEFDML
                     RVEGNKTEQDAGSPAAWNAFLATLNKDLTVPANYNAVVAQMDVDEFMDFFIAHTYSGN
                     LRGGEFRFWRATAPGSVWRWVLADLDNSFQKGQIKQDSLRVALQGNDVSIQPLKRMLA
                     NVNFRNAFAQRAASHLNITYAPARVTGLINAMRDEIAGEMPAHIARWKKPRTMTAWEA
                     EVTTMRNFANQRPDNMRNHLNTYLGSPGLANLTVAISGGGTVAVAGVEPLAFPFTGAY
                     YRNIPLTLAAVPAFGYRFVAWQETGETNAAISLTLSAATTRTAVFEPVPPPQLVINEI
                     HYNPADAQGADEVYEFIEIVNTGAEAVDLEGFGLADGISHTFGAGQSIAAGEIIVVAK
                     TAATYAALPGVQVFQWTSGDLSNGGETLTLKDASGNVIDQVAYDDDDLAVPPWPKTPD
                     GNGPSLSLLSPASNNNVATSWAASAQDGGTPGAVNFPP"
BASE COUNT          940 a         2132 c         1956 g          862 t
ORIGIN      
        1 cgacggcgcg acctatgccg ccgccggctg cgccgtctat gactggacgt cgggcgggct
       61 gaacagcggc ggcgagacga tcaccttccg caaccgcttc ggtgtgccgg tgctggcctt
      121 cgcctacggc acggccgcgc cctggccaac ggagccaaag ggcggcgggc cgtcgctggc
      181 cctgctcgac ctgacctacg acaacagtct gcccaccagc tgggccgcca gccgcgagac
      241 cggcggcacg ccctgcgcgg agaatttccc gccgctgccg cctgtggccg ccatcgtcat
      301 caacgagatc cactacaatc ccgacgacgc ggcccagggg ccggacgccg actatgagtt
      361 catcgaactg gtcaacacga gtggcgagcc ggtggcgctg gaaggctatg cgctgacggg
      421 cgtggactac accttcgccg ccggcgcgag catcgccgcg ggcgagcaca tcgtcatcgc
      481 cgccagcgcg gccacctacg ccggcagcta ccaggtcttc cagtgggacg cgggcagcga
      541 cctggccgac gacggcgaga cgctgacgct gaccgacggc tatggccgga cggtcgatga
      601 ggtgacctac ggcgacgcgg gcgactggcc ggccgcgccc gacggcgcgg ggccatcgct
      661 ggccttgcgc aaccccggcg acgacaacag cctggccgcc aactggaccg ccagcaacga
      721 gacgggcggc acgccgggag ccgcgaatgg attataacca gccggccggc cgtcccccgg
      781 acgcccgccc ggccccatgg aggcggccat gagacaacgg cgcatcgtgt tcatagcggt
      841 cctggcggcg ttgctggccg ggctggcgct ggcggcgcgc ctgcgggccg acgcgccgcc
      901 ggtggtcatt gccgaggtgc tggccggcaa tgcgtccacc aacctcgacc cggactacaa
      961 gaacttcgtg ccctgggtgg agttgcgtaa cgacggcgcg acggccgtca acctgaccgg
     1021 ctaccgcctg agcaacgacc tggccgtgcc caaccgctgg acgctgccgg ccggcgtcag
     1081 cattcccgcg gggggcgcgc tcttgctgtg gctcgacggc caggccacgg gccggcacgc
     1141 gcccttcgtg ctcgacatga gcggcgagct ggggctattc gcgcccgacg gcacgctgat
     1201 cgatagcctc acctttgggc cgcagcggcc cgacgtgtcc tatgggcgcg cccccggcgg
     1261 cgactggctg accttcgacc cgcccacgcc cggcgcggcc aacagcggcg gctgggccga
     1321 cccgacgccg gctggcccgc ccacgctcag cccgccgggc ggcttctatg ccggggcgca
     1381 ggcggtgacg ctggcggccg cccccggcgc gacgatccgc tacacgaccg acggccgccg
     1441 ccccacggcc gcctcgcccc tctacagcgg ccccatcgcc gtcaacgccc cgacggccat
     1501 ccgcgcccgc gccttcccgg ccggcggcct gcccagcgag acggccacgg ccacttacct
     1561 cgtcggcgtg tcgcccaacg tcgccgttgt ctccctggcc accgacccgg cctacttctt
     1621 cgatgacttc atcggcatct acgtcgatgg ccgggcgggc atcgccaatc ccgactgtgg
     1681 cccgaaggtg gccaactgga accgcccctg ggagcggccg gtcagcgtgg aaatctacga
     1741 cgccggcggg gcgctccttc tgcgacagga cggcggcgtg gccattgccg gcagttgcac
     1801 gcgcaaagac ccccagaaac agttgcaact cttcgcccgg cccggctatg gcgacgacga
     1861 tttcagcgtg gccctgtttg ccgacaagcc ggccgctgcc tatggccgca tcatcctgcg
     1921 ccccggcggc caggacgtgg ccaacaccct gctgcgcgac gccctgggcc agcagctcgt
     1981 cgccggccgg atggacattg accggcaggc ctatcgcccc ggcgtcgtct tcatcaacgg
     2041 ggccttctgg ggcatctacg gcgtgcgcga gcggatggac gagtactacg tcaccagcac
     2101 ctatggcctc ggcctgggcg aattcgacct gctggagaag aacgcccacg tcatggtcgg
     2161 ctcgcgggcc ggctgggacg cgctctacgc ctatctacgc gacaacaacc cggccacgcc
     2221 gtcggtctat gccaccgtgc agagccgcat cgacatcgac gagtacatca actatcagat
     2281 cgcggagatc tacgccgaca acatcgactg gccgcacaac aacatccgtt tctggagcgc
     2341 ccacgccggc ggccgttggc gctggatgct ctatgacatg gacgcggcct tcggccgcac
     2401 caccaaaggc tataacaaca acacgttcaa gttcgccgcg gccaagacgg gcaagcgggc
     2461 ctacaacgcc ctggtcttgc gccggctgct gcttagcccg gagttcaagg ccgagttcgg
     2521 ccagcgcttc atggcccatc tgaacacgac ctatgcgccg gcccgcgtcc gcggcatcat
     2581 cgacgacatg gccgcggcca tcgccccgca gatgccggcc caggtggccc gctggggcaa
     2641 acccaggtcg gtggccttct ggcaatcgga ggtcggccgg ctgcgcgtct tcgccgatca
     2701 gcgccccggc tatctgaccg gcttcctgaa taccttcctc ggcgcgccgg gaacggccgc
     2761 gctgaccgtc aaccacaatg ccgcgcaggg tcgcgtcctc gtggccgggg tcgatgtgcc
     2821 gggcaactat agcgggccgc acttccgcac gctgccggtg cgactggagg ccgtgcccgc
     2881 ggccggctat gtctttgtcc agtgggccga aacgggcgaa acgaacgcgg cggttgccgt
     2941 cgtgctcagc ggcgcggcgg ccacgcgcac ggccgtcttt gcgcccgcgc cctagcgcct
     3001 gtgcgcctgt cctgtctgtc ggagaatcgg ttatgaatcg tcatcgtctg tcgctcatgt
     3061 ttctcctggg ggcgctgctc ctggggctgg tggctgcgcc gcgcctgcgg gccgccgcgc
     3121 cgccgatcgt catcaacgag attctggccg gcaacgcctc ggtcaatctc gatccgcagt
     3181 tcaccaactt cgcgccctgg gtggagttgt acaacgcggg atcgtcggcc gtcagcctgg
     3241 ctggctacca tttgtccgac gacgccacca accccgccgg ctacacgctg ccggcgagcg
     3301 cgagcatccc ggcgggcggt cgcctgctgt tgtggtacga cgaaatggcc accggcgtgc
     3361 ataccagcct ctcgctggac atgcgcggcg acacggtggc cctgttcatg cccgacggca
     3421 cgctggtgga cgcggtgacc tatggctcgc agctcgacgg cgagacgctg gtcgatgtgt
     3481 cctatggccg ggccggcgac ggcgcgggcg cgtgggccag cttcgacacg cccacgcccg
     3541 gcgcggccaa cagcggcggc ctggccaccg ccgcgcggct ggcgcggccg gccttcagcc
     3601 cccccggcgg cgtctatgcc gccggccaga gcgtgaccct gacggccgaa ccgggggcgc
     3661 agatccgcta taccaccgac ggccgcaagc cgacggcggc ctcgcccctc tacaccggcc
     3721 ccatcaccgt caacacgccg gcggtcatcc gcgcccgcgc cttcgccgcc ggcgcgctca
     3781 gcagccgccc ggcgacggcg tcgtacctga tcggcgtgga tagtgacctg gccgttgtct
     3841 cgctggccac cgacccggcg aacctgttcg acccccagat cggcatctac gtcgtcggca
     3901 aaaacggggc caaggggcgt tgtgggccag tggccaactg gaaccgggcc tgggagcggc
     3961 cggtgagcct ggaactgtgg gagccgggcg gcgccttcgc cttccagcag gacgtcggct
     4021 tcgagattca gggcgattgc acgcgggcca tgccccagaa gtcgctggag atcaaggccc
     4081 gccggcgcta tggcgacaac gacatcgact atgccgtctt ccccggcaac ccgctggacg
     4141 aatatgagcg cctggtgctg cgcggcggcg gcaaccacaa cgcctacctg agcatgttcc
     4201 gcgagccgat ggttcaccgg ctgcatgagc agacgatggc cctcgaccag cagcagtacc
     4261 ggccgacggc cgtcttcatc aacggcgtct actggggcat ccacaatctg cgcgacaagg
     4321 ccgacgaggc gctcatcgag cagaactatg gcctggacgc cgacgacgaa ttcgacatgt
     4381 tgcgggtcga aggcaacaag accgagcagg atgccggcag cccggccgcc tggaacgcct
     4441 tcctggccac cctgaacaaa gacctgacgg tgccggccaa ctataacgcc gtcgtcgccc
     4501 agatggacgt ggacgagttc atggacttct tcatcgccca tacctacagc ggcaacctgc
     4561 gcggcggcga gttccgcttc tggcgggcca ccgcccccgg cagcgtctgg cgctgggtct
     4621 tggccgatct ggacaacagc ttccaaaagg gccagatcaa gcaggattcg ctgcgggtgg
     4681 cgctgcaagg caacgacgtt tccatccagc ccctgaagcg gatgctggcc aatgtgaact
     4741 tccgcaacgc ctttgcccag cgggcggcca gccatctcaa catcacctac gccccggcgc
     4801 gcgtcaccgg cctcatcaac gccatgcgcg acgagattgc cggagagatg cccgcccaca
     4861 tcgcccgctg gaagaagccg cggacgatga ccgcctggga ggcggaagtg acgacgatgc
     4921 gcaacttcgc caaccagcgg ccggacaaca tgcgcaacca cctcaacacc tatctcggtt
     4981 cgccgggcct ggccaacctg accgtggcca tcagcggcgg cggcacggtg gccgtggccg
     5041 gggtcgagcc gctggcgttc ccattcactg gcgcttacta tcgcaacatt ccgctgacgc
     5101 tggcggccgt gcccgccttc ggctatcgct tcgtcgcctg gcaggaaacg ggcgagacga
     5161 acgcggccat cagcctgacg ctgagcgcgg cgactacgcg gacggccgtc tttgagcctg
     5221 tgcccccgcc gcaactggtc atcaacgaga tccactacaa cccggccgac gcccagggcg
     5281 cggacgaggt ctacgagttc atcgagatcg tcaacaccgg ggccgaggcg gtcgatctgg
     5341 agggcttcgg gctggccgac ggcatcagcc acaccttcgg cgcggggcag tcaatcgccg
     5401 ccggggagat catcgtcgtg gccaagacgg cggcgaccta cgcggccctg cccggcgtgc
     5461 aggtcttcca gtggacgagc ggcgacctga gcaacggcgg cgagacgctg acgctcaagg
     5521 acgcctccgg caacgtcatc gaccaggtgg cctacgacga cgatgacctg gccgtgccac
     5581 cctggccgaa gacgcccgac ggcaacgggc cgtcgctgtc gctgctgtcg ccggccagta
     5641 acaataacgt ggccacgagt tgggcggcca gcgcgcagga cgggggcacg ccgggcgcgg
     5701 tgaacttccc gccgtaaggg gttcctcccg cctccgccct tcgggagggg gtgggggctg
     5761 cgccacgcgg tgaatcacct gcaaatggtg acattcgtca ctataaggcc gtgtggccgt
     5821 gtgttatact cgttagtgtc gctgaacagt tctcgttgtg ggctgggagg gcgcgccgat
     5881 agggagccgg
//
LOCUS       DAOSMQ010000164         5888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13695_length_5888_cov_4.841762, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000164 DAOSMQ010000000
VERSION     DAOSMQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13695_length_5888_cov_4.841762"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PK829_06905"
     CDS             complement(<1..178)
                     /locus_tag="PK829_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOU40981.1"
                     /translation="MDRIDFRSDTVSWPTPVMRAAMANARVGDDVYGEDPTINELEAL
                     AAARVGKEAGLFVAS"
     gene            complement(395..1399)
                     /locus_tag="PK829_06910"
     CDS             complement(395..1399)
                     /locus_tag="PK829_06910"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU40982.1"
                     /translation="MKRATLIYNPRAGQLFAAAKIEPVADAWRARGWHITLRATARPG
                     HATELARAAAEAGEQVVLAAGGDGTLGQVADGLAGSDTVLAPLPMGTANALARELRLP
                     RLHILDPDAPVTAANALLDGRVQHVDLNYVQTRGWAGHALLWAGIGADGYLVERLEPR
                     PTWSKRLGPVGYSIQALSVLHSLPTLNAVVQVDDQIVEGDWLLIVISNCRLYAGGWLQ
                     LSSAAVFDDGTFEVWLFRAGQVSARLMPPRVGLMARYLTGVGLNLQAWDPGVLNLTGK
                     RVVVEAQGRVPCQRDGEPAGYTPLAAEIRPKALRLLVPPAAPKELFARPGVPLVELFP
                     "
     gene            complement(1426..5214)
                     /locus_tag="PK829_06915"
     CDS             complement(1426..5214)
                     /locus_tag="PK829_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40983.1"
                     /translation="MRRLGFSRPAGVLLLLGGLVVAIAVCALVARLADVLPALGGGAP
                     AAPVRLAGLPTPVVTRPEWTSYTYAGPVRDLALSGGLLWAATDGGLVVWDGAGNAARF
                     TTEHGLPANRVTSLVIGADGAIWAGTVAGLGRYDGRVWQTFTTADGLPADAIRDLAAD
                     RDGNVWAATAAGLALTDGNGWRVTGRGGLFGSLPSQDVTALAVDTANRLWVATAAGLS
                     RLEGRRWTTFTAADGLPVEPIRALAAGAGDVVWGMTDVTLVRADGSVFAHFTPGLTGE
                     AMTATYTAVAAAADGTVYLAIDPNNRAITRFDPATLAEERLDIAPRPVASDTLGALLV
                     DAQGALWAGVGDTLRRYAGDWTTLAAPSDLPAPALTALAYAHGSVWAASAGGVTRLDG
                     GGQTFGVADGLAGDDVRALAVASDGTLWAASDTPLRGLSRYGPDGWQTVTCPTAAPLS
                     ADVSDAAQTPSAVWLATPRGIGRFDGAAWRTYDARDGLPDAPANALAAAGEIVYAATD
                     AGIARFDGAAPDGRWQMVSDAPARDLAIAPDGDLWATDGLAVALVGEDTASALPLPTT
                     VRGMAAADGAVWLATPDGVLRYDGRWTIFTAADGLPTLDVTAVGAAADGHVWAATSGS
                     ANGIEMVQFDGRRWQPHPNRDPAAEQLAEATVYRILATPDGDIWLGTLAGVERYHDSR
                     WSVYATDSGLPDDFISALAYAYGQVWAGTPRGLARFTGAGWELFGAPSADAVGPAVSA
                     LAVAPSGELWVALDAAQTNRLRVYDGQLWRVMPLPAPFAFLTQMAFTPAGQLLAIVVD
                     NGTTVLGLFDGDTWTLATVADLGLEPRALGVSPDGRRLWLTGLVPNAGAAPPDAVAEL
                     LPGQGTAAAAVVVLALGPDGLGPEIGRFVAADIVAYDWEYAGAVQPIAFGADGRVTVA
                     GSGQVYVFDGAAGAIAPVATRALPLPFTRTTFALASAPDGRLWVGTEGGLAALDGETW
                     QSFYAPARAPEWWGSATVLWPRADGGVLVGTSGGGVGIYTGHDFEGVMRPSEGPAEWA
                     GAFLPVTALLFRGEGELWATSAAGAARLTEAWEVFVPDATLAAETTGLAATAGRLWLG
                     TAAGWIAVELTGDACRYAAVEPLAGVAALLTDQQDAVWLAAADGVLRAPAGGAPSPVL
                     DSTMLVMTLSPNGEVWLGGQRQPWLMRYRPGDGEGSWSRLPLDMSLMPAQSLKALAVA
                     PDLDLWLGGDGLVRFRGGVWSKLTTADGLADNRVDHVLVTPDGAVWAATAGGLSRYRP
                     "
     gene            5491..>5888
                     /locus_tag="PK829_06920"
     CDS             5491..>5888
                     /locus_tag="PK829_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOU40984.1"
                     /translation="MTTEQEIWTFLDRHLRSIFSRDVEAYRATTGDDLSLYEWFVAPH
                     RQDGLDFHFFMIEHSWAGAGADYRYDLLEPRLQLYGDTAIVSYTFMLTVAGETGIRHT
                     LHNESRVLVRRDGAWQVVHVHKSPAWRAPR"
BASE COUNT          877 a         2198 c         2051 g          762 t
ORIGIN      
        1 cgctggcgac gaacagcccg gcctccttgc ccacccgcgc cgccgccagc gcttccagtt
       61 cgttgatcgt cgggtcttcg ccgtagacat cgtcgccgac acgggcatta gccattgccg
      121 cgcgcatcac cggcgtcggc caactgaccg tatccgagcg aaagtcgatc ctgtccatag
      181 gcgctagttt acgtcaacct gggccgattg ggtagcaggg cttttccaaa gtcatctagc
      241 gaccatgacc tgacaggtgt cgttctctgg tggcagtccc atgcgcggcc gcccatctgt
      301 caggtcggcg cgcctggggt gactctggca aagccctgga ttcggtagtc tgagcggccc
      361 gccggccagg cccaggtcaa agacggtggc agcgctaggg gaacagttcc accaaaggca
      421 caccgggccg ggcgaacagt tcctttggcg cggccggggg cacgagtaac cgcagcgcct
      481 tcggccggat ttcagccgcc agcggcgtat agccggccgg ctcgccatcg cgttggcagg
      541 gcacacgccc ctgcgcctct acaaccacgc gcttgcctgt gaggttgagc acgccggggt
      601 cccaggcctg caagttcagc ccgacgccgg tcagatagcg ggccatcagg ccgacgcgcg
      661 gcggcatcag ccgcgccgat acctgcccgg cgcggaacag ccacacctcg aacgtaccat
      721 cgtcgaatac cgcggcgctg ctcagttgca gccagccgcc ggcataaagc cggcagttgc
      781 tgatgacgat cagcaaccag tctccctcga cgatctggtc atcgacctgg acgacagcgt
      841 tcagcgtcgg caggctgtgg agcacgctca gggcctggat gctgtagccg accggaccca
      901 ggcgctttga ccacgtcggc cgcggctcca gccgctcgac gaggtagccg tcggcgccaa
      961 tgcccgccca gagcagggca tgcccggccc aaccgcgcgt ctgcacgtag ttgagatcga
     1021 cgtgctgcac gcggccatcg agcagagcgt tggccgccgt caccggcgcg tcggggtcaa
     1081 ggatgtgcag gcggggcagg cgcaactcgc gggccagggc gttggccgtg cccatcggca
     1141 ggggagccaa caccgtgtcg ctgccggcca gcccatcggc cacctggccc aatgtgccgt
     1201 cgccgcccgc ggccaggacg acctgctccc cggcctcggc cgcggcgcgg gccaactcag
     1261 tggcgtggcc ggggcgcgcc gtggcccgca gcgtaatgtg ccagccgcgg gcgcgccagg
     1321 cgtcggccac tggctcgatc ttggcggcgg cgaatagctg cccggcgcgt gggttgtaga
     1381 tgagggtcgc tcgtttcatg actaacttgc tgcccgtcgc gccgctcagg ggcgatagcg
     1441 gctcagtccc ccggccgtcg cggcccagac agcgccatcg ggcgtcacca gaacgtgatc
     1501 aacccgattg tcggccagtc catcggcagt tgtcaacttc gaccagaccc cgccacgaaa
     1561 acgcaccagc ccatccccgc ccagccacag gtcgaggtcg ggggcgacag ccaaagcctt
     1621 tagcgattgg gccggcatca ggctcatgtc gagcggcaac cggctccaac tgccctcgcc
     1681 gtcaccgggc cggtagcgca tcagccaggg ctggcgctgc ccacccagcc agacctcccc
     1741 gttgggcgac agggtcatga ccaacatcgt gctatcgagg actggcgatg gcgcgccgcc
     1801 ggccggggcg cgcagaacgc cgtcggccgc ggccagccag acggcatcct gctggtcggt
     1861 cagcagggcg gccacgccgg ccagcggctc cacggccgca tagcggcagg cgtcgccggt
     1921 gagttccacg gctatccagc ccgcggccgt ccccagccac agccggccgg ccgtcgccgc
     1981 cagccccgtc gtctcggccg ccagcgtggc atcgggcacg aagacctccc atgcctccgt
     2041 caggcgggcc gcgccggcgg ctgacgtggc ccacagctcg ccctcgccgc ggaacagcag
     2101 cgccgtgacc ggcaggaacg cgccggccca ctcggccggc ccctccgagg gccgcatgac
     2161 gccctcgaag tcgtggccgg tgtagatgcc cacgccgccg ccgctcgtgc ccaccaggac
     2221 gccgccatca gcccgcggcc atagcaccgt ggcgctgccc caccactccg gcgcgcgcgc
     2281 cggcgcgtag aagctctgcc acgtctcgcc gtccagcgcc gccaggccgc cctctgtgcc
     2341 cacccacagg cggccgtcgg gcgcggacgc cagggcgaag gtcgtccgtg tgaagggcag
     2401 cggcagcgcg cgcgtcgcca ctggcgcgat ggcgccagcg gccccgtcaa agacgtagac
     2461 ctgacccgac cccgccaccg tcacgcgccc gtccgcccca aaggcgatgg gctgcaccgc
     2521 gccggcgtac tcccaatcgt aggcgacgat gtcggcggca acgaagcggc cgatctctgg
     2581 ccccagaccg tctggcccca gcgccagcac gacgacagcc gcggcggctg tgccctgccc
     2641 gggcagcagc tcggcgaccg cgtcgggcgg cgcggcgccg gcgttcggga cgaggccggt
     2701 caaccacagc cgccggccat cgggcgagac gcccagcgcg cgcggctcta gccccaggtc
     2761 ggccacggtc gccagcgtcc acgtgtcgcc gtcgaaaagg cccagcaccg ttgtgccgtt
     2821 gtctaccacg atggccagca gttggccggc gggagtgaag gccatctggg tgaggaaggc
     2881 gaagggcgcg ggcagaggca tgacccgcca caactggccg tcatagacgc gcagccggtt
     2941 tgtctgcgcg gcatcgaggg ctacccatag ctcgcccgac ggggccacgg ccagcgccga
     3001 cacggccggc cccacggcat cggccgaggg cgcgccgaat agctcccagc ccgcgccggt
     3061 gaagcgggcc agcccgcgcg gcgtcccggc ccacacctgg ccgtaagcgt aggccagggc
     3121 gctgatgaaa tcgtcgggca ggccgctgtc ggtcgcgtag acgctccagc ggctgtcgtg
     3181 gtagcgttcg acgccggcca gcgtccccag ccagatatcg ccgtcgggcg tggccagaat
     3241 gcggtagacc gtcgcctcgg ccagttgctc ggccgccggg tcgcggttgg ggtgcggttg
     3301 ccagcggcgg ccgtcgaact ggaccatttc gatgccgttg gcgctgccgc tcgtcgccgc
     3361 ccaaacgtgg ccatcggccg ccgcgccgac ggcggtcaca tcgagcgttg gcaggccgtc
     3421 agcggcggtg aagatcgtcc agcggccgtc gtagcgcagc acgccgtcgg gcgtggccag
     3481 ccacacggcc ccgtccgccg ccgccatgcc gcgcacggtt gtcggcaggg gcagggcgct
     3541 ggccgtgtcc tcgcccacca gggcgacggc caaaccatcg gtcgcccaca ggtcgccgtc
     3601 cggggcaatg gccaggtcgc gggcgggcgc gtcgctgacc atctgccagc ggccgtcggg
     3661 cgcggcccca tcgaagcggg cgatgcccgc gtccgtggcc gcgtagacaa tctcgccggc
     3721 ggcagccagg gcgttggccg gcgcgtcggg cagtccgtcg cgagcgtcgt aggtgcgcca
     3781 ggccgcgccg tcgaagcggc cgatgccgcg cggcgtggcc agccagacgg ccgatggcgt
     3841 ctgcgccgcg tcgcttacgt cggcgctgag aggcgcggcc gtggggcagg tcaccgtctg
     3901 ccagccgtcc ggcccatagc gcgacaggcc gcgcagcggc gtgtcgctgg ccgcccacag
     3961 cgttccatca gaggcgacgg ccagggcacg cacgtcgtcg cccgccaggc catcggccac
     4021 gccgaacgtc tgcccgccgc cgtccagccg ggtgacgccg ccggccgagg ccgcccagac
     4081 gctgccatgg gcgtaggcca gcgcggtcag agccggggcg ggcaggtcgg aaggcgcggc
     4141 cagcgtcgtc cagtcgccgg cgtagcgccg cagggtgtcg cccacgccgg cccaaagcgc
     4201 gccctgcgcg tcgacaagca gcgcgccgag ggtgtcgctc gccaccggcc gcggggctat
     4261 gtcgaggcgc tcctcggcca gcgtcgccgg gtcgaaacgg gtgatggcgc ggttgttggg
     4321 gtcgatggcc aggtagaccg tgccgtcggc ggccgcggcc acagccgtgt aagtcgccgt
     4381 catcgcctcg ccggtcaggc cgggggtgaa atgcgcgaac acgctcccgt cggcgcgcac
     4441 cagggttaca tcggtcatcc cccagacgac atcgcccgcc cccgccgcca gcgcccggat
     4501 cggctcgacc ggcaggccgt cggcggcggt gaaagtcgtc caacggcggc cctccagccg
     4561 cgacagcccg gccgccgtgg ccacccacag ccggttggcc gtgtccacgg ccagcgcggt
     4621 gacatcctgg ctgggcagcg acccgaagag gccgccgcgg ccggtgacgc gccagccgtt
     4681 gccgtccgtg agggccaggc cggcggccgt ggccgcccag acgttgccgt cgcggtcggc
     4741 ggccaggtcg cggatggcgt cggcgggcag gccgtcggcc gtggtgaagg tctgccagac
     4801 gcggccgtcg tagcggccca gccccgccac cgtgcccgcc cagatcgccc cgtccgcgcc
     4861 aataaccagg ctggtgacgc ggttggcggg cagcccgtgc tccgtcgtga agcgggccgc
     4921 gttacccgcg ccgtcccaga cgacaaggcc gccgtcggtg gccgcccaga gcaggccgcc
     4981 gctcagggcc aggtcgcgca ccggcccggc gtaggtgtag ctcgtccact ccggccgggt
     5041 gacgacgggc gtgggcaggc cagccaagcg caccggggcc gccggcgcgc cgccgcccag
     5101 cgccggcagc acgtccgcca gccgcgccac cagcgcgcag acagcgatgg ccaccaccag
     5161 gccgcccaga agcaacagca cccccgccgg ccgcgagaaa ccgagccgtc tcatagcgcc
     5221 aattgtagcg gccgctggca gcccggcgaa tccgcccgcc agggccattg ccaggtcaat
     5281 aggaactcac gccaggcagc tcacgcaaag ccgcaaagga agcaaaggcg caaaggaaga
     5341 aagccgctta ttcttcttgg cgtccttggc ttcctttgcg ccttggcgtg agcgtttggc
     5401 gtgatcgctt cgcaacccat gcccccgcca gacttggcct tcccggtcgt cttgggctag
     5461 attccgccta cttcacccag gagacaacac atgaccacgg aacaggagat ttggactttt
     5521 ctcgatcgcc acctgcgcag catcttcagc cgcgacgtgg aggcctaccg ggcgacgacc
     5581 ggcgacgacc tgtcgctcta cgagtggttc gtcgccccgc accggcagga cgggctggac
     5641 ttccacttct tcatgatcga gcacagttgg gcgggcgcgg gcgcggacta ccgctatgac
     5701 ctgctggagc cgcgcctcca actctatggc gatacggcca tcgtcagcta caccttcatg
     5761 ctgaccgtcg ccggcgagac gggcatccgc cacacgctcc acaacgagag ccgcgtgctg
     5821 gtgcggcgcg atggcgcgtg gcaggtcgtc cacgtccaca agtcgccggc gtggcgcgcg
     5881 ccgcggga
//
LOCUS       DAOSMQ010000165         5843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13822_length_5843_cov_5.431064, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000165 DAOSMQ010000000
VERSION     DAOSMQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13822_length_5843_cov_5.431064"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(439..1554)
                     /locus_tag="PK829_06925"
     CDS             complement(439..1554)
                     /locus_tag="PK829_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40985.1"
                     /translation="MTPEPPLPTRTARPGPLTPPLTPVPCPTRAADEYVPLMWERSVA
                     LPLDISLANAHYAFLPPHDFFIGVENRIGYVRLDEPARMTSDPFSIFVYGFAEIVELP
                     PITDIEAQADSLLYLTGGSQLTVVHTGYLCYLAPVATADFPFPAQDVELEGERVYVGG
                     SDGQQLHVAVLDRTALPEIRQLAALTFPVGDWSVVGKQLLTVDSETAAVTVSDVFDLS
                     AVTTRQVAVPFDAQWNVMGPPRLFEDTLWLMVWDKGLMTVTGLLDAEPMVLWREFHSY
                     FAMDFVTMQETHTFVADNWCDAGNCAAYVDIAGRNPEGEFAQFSLYPHHPVFHYHAIG
                     NNIVWAFSDYSLIVIDLTAPAGQEIVRTYPLRGRLDQ"
     gene            2159..4657
                     /locus_tag="PK829_06930"
     CDS             2159..4657
                     /locus_tag="PK829_06930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU40986.1"
                     /translation="MVSKPLAPLVLLLALLLLGTWLRVHNLDAFSFWTDEGLTPARSG
                     YPLAQILRNEIVIQGVVTTDTHPPLYYLIIAVTRRLFGLSDFAFRYPSVLFGVLLIPL
                     LYQFGRALLSSQTPSPLGRGTATGGPAITLGLLVALLATVNPLQVYYSQEARMYTLLV
                     LLATGMSIVLWRALREEKGLTQRRKDAKKNPFALAFLASWREHPLFRHLLTYIILAAL
                     AVYTHYTAVFLVAAQALFWAWLLWRAGWRWLIIGALVVAALAAIPLIPYTLPRLRSGA
                     EANYFPVSPLTMLQDVVRFFNLGLTVDFGRPVIILLNGLALALLLLGVWVAGCPLHSP
                     RPGGDGGLSPAPPLPRSPALLRPAFLLVWLLAPVLGLMLGSALFKPMYQGVRHIMAAS
                     PAFLLLVGLGIWAIWGRFAKSPYKPLALVPLVLVLLGAALSLLALYTDPAYAKDDFRA
                     LVRFIETRAGGNDAIVYNNAVLLPLHEHYRLRGDLAVTALPAYPQVATGQEPELAALA
                     ATYDRLWLVTDPPTDNRDDDGLIRAWLDDNLLEVADRLFPARTTEARVLGYATAFNGQ
                     WSAASGQWAGLPALAHAAIDRSPLALPALWVDLWWQGDAPPDGAQLVFTLVGPDGAEY
                     VRRAHPLRRPGAPWDAADNRFSYDLPLPPGLPPGEYALAVGPAGQPAAALGAVTVEPT
                     TAWPAAPEALFARTDLAVLGRRPAATWPNGLALAAVEPWDDHAAAGNNLPLTLFWRAG
                     PAGIDLTALRYRLEVIDRSGAALRAQETRPGAPWLGEVPAGALLREVTSLYFRPETPP
                     GRYRLRWTLLDSDAVVGQPVVAGQVVVTAPEATP"
     gene            4654..>5843
                     /locus_tag="PK829_06935"
     CDS             4654..>5843
                     /locus_tag="PK829_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40987.1"
                     /translation="MTRERRLLALILLGYLVVTLAYGAVNPLFEAPDEHWHFFTAESI
                     ARTGRLPRVETPPDEWLGQEAAQPPLYYLLGAALIAPIDTGGAREAVWLNPFAWIGDA
                     SALNNVNRMVHPPEERWPWQGYVLAGHVLRVFSTLLGLGTLLAIYGSARRLWPDSRTR
                     VLMAPALVAFLPQFNFIHASITNDALITFLCSLALYQLIGLWRAPVGEELTQRTPREP
                     RTPRRGLFLRPLPPSRPWRELLLGITIGLAALTKNAGIALLVYSLGFLALLALRDGRP
                     REFLRTALLVAGPALLLAAPLWARNAALYGDWTATAPFIAIAGGDRGYTLGQALGEWQ
                     GLALSLVGVFGWFNLRPPAWVYWVWLAIVVLAIAGALGCAALAQRRRVSLSSPPPPRP
                     PAPRL"
BASE COUNT          872 a         2049 c         1885 g         1037 t
ORIGIN      
        1 cttacattct cgtgacagag aatcggcgca caatgccgtc tcatttggca cgccactatg
       61 aagaaggtca ctccatctcc ggcattctgt ggattcgacc gcggtcttca attggccaaa
      121 tcgtagaagc gcttttctaa ttgtggttaa gctccactca agaacaattc cagcacagga
      181 acgaatttat tccctagcgt tacattaaga ccctctaccg cacaaacgac tgcaaaaacg
      241 caaccccaaa cacagcccca cccccgccct tgggctgccc acaagaccat actccccatc
      301 gaaggccacg cgcatgcccg ccgctggccg ccgttcgccg caaccggcca acgcgagcga
      361 ggcggcaatg aagacgaccc aatcaacaag ccgtcgcctt ctagcgctac atgacttcgg
      421 ttcggtgctc acggcgcgct actgatccag cctcccccgc aacggatacg tccgcacaat
      481 ctcctgcccc gccggcgcag tcaggtcgat gacgatcagc gagtagtcgg agaaggccca
      541 aactatgttg ttgccgatcg cgtggtagtg aaagacgggg tggtggggat agagcgagaa
      601 ttgggcaaat tcgccctccg ggttgcgacc agcaatgtcg acgtaggcag cgcaattacc
      661 cgcatcgcac caattatcgg ccacaaaggt gtgcgtctct tgcatggtga cgaaatccat
      721 cgcgaagtag gagtgaaatt cgcgccatag caccatcggc tccgcgtcga gcagcccggt
      781 gaccgtcatc agccccttat cccaaaccat cagccagagt gtatcctcga atagccgcgg
      841 cgggcccatc acgttccact gggcgtcgaa gggcacggca acctggcgag tggtcacggc
      901 gctcaggtcg aacacatcgc tgacagtgac cgccgccgtt tccgaatcaa cggtcagcag
      961 ttgcttcccc accaccgacc agtcgccaac cgggaaggtc aacgccgcta gttgccggat
     1021 ttcaggcagg gccgtccggt cgagcacggc cacgtggagt tgctgcccat cggagccgcc
     1081 gacgtagacg cgctccccct ccagttccac gtcctgcgcc ggaaaaggga agtcggctgt
     1141 cgccaccggg gcgaggtagc atagatagcc agtgtggacg accgtcaact gcgaaccgcc
     1201 ggtcaagtag agcaggctgt cagcctgcgc ctcaatgtcg gtaatgggcg gcaactcgac
     1261 tatctccgcg aaaccataca cgaaaatgct gaaagggtca ctggtcatcc gcgccggctc
     1321 atccagcctg acgtagccga tccggttctc caccccaata aagaagtcgt ggggcggcag
     1381 gaaggcgtag tgggcgttgg ccaaggagat gtccagcggt aatgccaccg accgttccca
     1441 catcaggggc acatactcat ccgccgcgcg cgtcgggcag gggacgggcg tcaatggcgg
     1501 cgtaagcggg ccgggccggg cggtgcgcgt gggcaggggt ggctccggcg tcatgtaccg
     1561 gccgtgctcc tgttcgtcgc cgcgtgttgg cgaaggcagc ggggaagttg gggtggttgt
     1621 cggcgtggaa ttgagagtgg ttgaggtcgg cgcgatggtg ggcgtcgccg tcgctacggc
     1681 gggtgcggcg gccaatgact ctggtgtgtt cgctgggggc gtgaccacag tcgctacggc
     1741 cgccggttcc aacgccggcg gcgattccgt ccccgaacga cacgcggcca gcaaggccca
     1801 ggcgagaacg agagcaccaa gaaaggcaat ggacgcgcgc cgcagcggga acatcgctca
     1861 accctccggg cgcggccaat gctgccgcgc cgctatcgat gaagccatca tagcgcaagc
     1921 gggcaacgcc tgcccattgc ccgcccatcg cttcccccac gctcgtgcgt ggcgcgattc
     1981 tctgaatcgc gttctttcgc agagcgctcg cgcgtggcgc gattctccga atcgcgttct
     2041 ttcgcagagc gattcggaga atcgcgccac gatgcccgcg tgccgatcgc gccggcacgc
     2101 gctagaatct accgttatgg ttgcccacag cgtagaaacc gagtttctca gaagaaactt
     2161 ggtttctaaa ccgcttgccc cgctcgtcct cctccttgcc ctcctcctat tgggcacctg
     2221 gctgcgcgtc cacaacctcg acgccttcag cttctggacc gacgaggggc tgacgccggc
     2281 gcgctccggc tatccgctgg cccagattct gcgcaacgag atcgtcatcc agggcgtggt
     2341 gacgaccgac acgcacccgc cgctgtacta cctgatcatc gccgtcacgc gccggctgtt
     2401 cggtctgtcg gacttcgcct ttcgctatcc gtcggtgttg tttggcgtgc tgctcattcc
     2461 gttgctctac cagttcggcc gcgcgttgtt gtcctcccag accccctcgc ccctggggag
     2521 agggacggca acgggagggc cggccatcac cctcggcctc ctcgttgctc tgctggccac
     2581 agtcaacccg ctgcaagtct attacagcca ggaagcgcgc atgtacaccc tactcgtcct
     2641 gctggcgacg gggatgagca tcgtcctgtg gcgcgcgctg agggaagaaa aggggctcac
     2701 gcaaagacgc aaagacgcaa agaagaatcc ttttgccttg gcgttcttgg cgtcttggcg
     2761 tgagcatcca ctttttcgtc atctgctgac ttacatcatc ctggccgcgc tggccgtcta
     2821 tacgcattac accgccgtct ttctcgtcgc cgcgcaggcg ctcttctggg cgtggttgct
     2881 gtggcgggcg gggtggcggt ggctgattat cggcgcgctg gtcgtcgccg cgctggcggc
     2941 tatcccgctc atcccctaca cgttgccgcg cctccgcagc ggggccgagg ccaactattt
     3001 tccggtctcg ccgctgacga tgttgcaaga cgtggtgcgc tttttcaacc tggggctgac
     3061 ggtcgatttc ggccggccgg tcatcatcct gctcaacggg ctggcgctgg cgctgctgct
     3121 gctgggcgtt tgggtggccg gttgccccct ccacagccct cgcccagggg gcgacggcgg
     3181 cctctccccc gctcccccgc tcccccgctc ccccgctctt ctacggccgg ccttcctgct
     3241 cgtctggctg ctggcccccg tgctcggcct gatgcttggc tcggcgctct tcaagccgat
     3301 gtaccagggt gtgcgccaca tcatggccgc cagcccggcc ttcctgctgc tggtcggcct
     3361 gggtatctgg gcgatctggg ggcgatttgc caaatcgccc tacaagccgc tggctctcgt
     3421 gccgttggtg ctggtgctcc tcggcgcggc gctgtcgttg ctggccctct acaccgaccc
     3481 ggcctacgcc aaggacgact tccgcgctct cgtccgcttc atcgagacgc gcgccggcgg
     3541 caacgacgcc atcgtctaca acaacgccgt cctgctgccg ctgcacgaac attatcggct
     3601 gcgcggcgat ctggccgtta cggcgctgcc ggcctacccg caagtggcca ccgggcagga
     3661 gccggagctg gccgcgctgg cggcgaccta tgaccggctg tggttggtca ccgacccgcc
     3721 gaccgacaac cgcgacgacg acggcctcat ccgcgcctgg ctcgacgaca acctgctgga
     3781 ggtcgctgac cgcctcttcc cggcgcgcac gaccgaggcg cgggtgctcg gctacgcgac
     3841 ggcgttcaat ggtcagtggt cagcggccag cggccagtgg gctggcctgc ccgcgttggc
     3901 ccacgccgcc attgaccggt cgccgctggc gctgccggcg ctgtgggtcg atctgtggtg
     3961 gcagggcgac gcgccgccgg acggggcgca actggtcttc acgctggtgg ggccggacgg
     4021 ggccgagtac gtccgccgcg cccatccgct gcggcggccg ggcgcgccct gggacgccgc
     4081 cgataaccgt ttcagctatg acctgccttt gccgccgggc ctgccgccgg gtgagtacgc
     4141 cctggccgtc gggccggcgg ggcagccggc cgcggcgtta ggcgcggtga cggtcgagcc
     4201 gacgacggcg tggccagccg cgccggaagc gctgttcgcc cgcaccgacc tggctgtgct
     4261 gggtcgccgg ccggcggcca cgtggcccaa cgggctggct ctggcggcgg tcgagccgtg
     4321 ggacgaccac gccgccgccg gcaacaacct gcccctgacg ctgttctggc gcgccggccc
     4381 ggcgggcatt gacctgacgg cgctgcgcta ccggctggag gtcatcgacc ggagcggcgc
     4441 ggcgctgcgg gcgcaggaga cgcggcccgg cgcgccgtgg ctgggcgagg ttccggccgg
     4501 ggcgctgctg cgcgaggtca ccagcctcta cttccggccg gagacgccgc ccggccgcta
     4561 ccggctgcgc tggacgctgc tggacagtga cgccgtcgtc gggcagccgg tcgtggccgg
     4621 tcaggtcgtc gtcaccgcgc cggaggccac gccatgaccc gcgaacgccg cttgctcgcc
     4681 ctcatcctgt tgggctatct cgtcgtcacc ctggcctatg gcgcggtcaa cccgctcttc
     4741 gaagcgccgg acgagcactg gcacttcttc acggccgagt ccatcgcccg caccggccgg
     4801 ctgccgcgcg tggagacgcc gccggacgag tggctggggc aggaggccgc ccaaccgccg
     4861 ctctactacc tgctcggcgc ggccctcatt gctcccatcg acactggtgg cgcgcgcgag
     4921 gccgtctggc tcaacccgtt cgcctggatc ggcgacgcct cggcgctgaa caacgtcaac
     4981 cgcatggtgc atccgccgga ggagcgctgg ccgtggcagg ggtacgtgct ggccggccac
     5041 gtgttgcgcg tcttctcgac gctgctgggg ctgggcacgc tgctggccat ttacggcagc
     5101 gcccgccgcc tctggccgga cagccgcacg cgcgtgctga tggccccggc gctggtggcc
     5161 ttcctgccgc aattcaactt catccacgcc tcgatcacca acgacgcgct gatcaccttc
     5221 ctgtgcagcc tggccctcta ccaactcatc ggcctgtggc gcgcgccggt gggggaggag
     5281 ctcacgcaaa ggacgccaag ggagccaaga acgccaagaa gaggcctctt tttgcgtcct
     5341 ttgccccctt cgcgtccttg gcgtgagctt ctgctgggca tcacgatcgg cctggcggcg
     5401 ctgaccaaga acgcgggcat cgccttgctg gtctatagcc tcggctttct ggcgctgctg
     5461 gccctgcggg acggccggcc gcgtgaattc ctccgcacgg cgctgctggt ggcagggccg
     5521 gcgctgctgc tggccgcgcc gctgtgggcg cgcaacgctg ctctctatgg cgactggacg
     5581 gccacagccc ccttcatcgc catcgccggc ggcgaccggg gctacacgct ggggcaggcg
     5641 ctgggcgagt ggcaggggct ggccctgtcg ctggtgggcg tcttcggctg gttcaacctc
     5701 cggccgccgg cctgggtcta ctgggtctgg ctggccatcg tcgtcctggc catcgccggc
     5761 gcgctgggct gcgccgccct ggcccaacgt cgccgtgtct ccctctcttc tcccccgccc
     5821 ccccgccccc ccgccccccg gct
//
LOCUS       DAOSMQ010000166         5839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13832_length_5839_cov_4.311376, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000166 DAOSMQ010000000
VERSION     DAOSMQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13832_length_5839_cov_4.311376"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK829_06940"
     CDS             <1..318
                     /locus_tag="PK829_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU40988.1"
                     /translation="QMMIKYGSELVPAVRTAKFRAAWSAARPLIGPRGADTGRELSRT
                     FKCFDHIDEGVEGFVTITGGKATTLRGMAETTANIVCQKVGFPVPCPTRDYVLLPHTA
                     YYV"
     gene            437..1156
                     /locus_tag="PK829_06945"
     CDS             437..1156
                     /locus_tag="PK829_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOU40989.1"
                     /translation="MTERDIEFKVFRYKQGGAAPTYQTFTSRCNENTTVLVALQDIRR
                     DQDGTLILRHSCHHASCGTCGMRINGREELACVVKVFDLGTPTVVVEPMRNMPVISDL
                     VVDMEPFYDRFNSAGMPFIRESEYLEEAEVAEGLETYMRCENCIECNLCVSACPIMGT
                     DPTYLGPAALAAAWRVVEEPRGLDSRPLLDWVDSQNGCWRCHVAYECNEACPSGVFPG
                     DKIMSLRRELTKRKFRRLFGR"
     gene            1232..1636
                     /gene="sdhC"
                     /locus_tag="PK829_06950"
     CDS             1232..1636
                     /gene="sdhC"
                     /locus_tag="PK829_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOU40990.1"
                     /translation="MDDIQPTTDPVRPKAPLRSWFDVRGRSLSTLAFALNRLTAIGLT
                     VYLFLHLAVLSMLLGGPESWNRFLAVARSPFFLLLDVVLLFGVVYHMANGIRVALVGM
                     GVGHTQHKTMFYVLVGLALVLFVVGAALIFVG"
     gene            1660..2046
                     /locus_tag="PK829_06955"
     CDS             1660..2046
                     /locus_tag="PK829_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40991.1"
                     /translation="MSRRTLEEMATRRDRSGSLWLVQAFTGLLLVFILGAHMIAHHFI
                     VEGGLRDYQQVLDYVANPFVFIIEVVFVIIATIHALLGARAIVLDLRPPAATVKVINW
                     VLVAIGAVAIGYGLWLALALQRAAGA"
     gene            2046..2258
                     /locus_tag="PK829_06960"
     CDS             2046..2258
                     /locus_tag="PK829_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40992.1"
                     /translation="MAKKQAAPPPPPQTNWRWYWIRTALITVLMFIFVLWFMLPRSGS
                     VAYLWATVAAVAILGYFVVSYFMLRR"
     gene            2298..2888
                     /locus_tag="PK829_06965"
     CDS             2298..2888
                     /locus_tag="PK829_06965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU40993.1"
                     /translation="MGSDATWEARWAPYDEETYARALAYVPPGAVVLDIGAGDLRFAR
                     RLAARARLVYAVERRPELLAGAAAWPGDVGNVRAVCADARDWPFPPSLDAAVLLMRHC
                     AHAHFALYGRKLAATGCRWLITNARWRMGVECIDLSAPAQPYERVAVGWYACRCGATG
                     FRAGPVEALTEAALAEVYEVEGCPECKDGWSGLRLP"
     gene            2860..5391
                     /gene="ptsP"
                     /locus_tag="PK829_06970"
     CDS             2860..5391
                     /gene="ptsP"
                     /locus_tag="PK829_06970"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOU40994.1"
                     /translation="MVGLVFVSHSAQLAEGVLEFAGQMAQGAVRMAAAGGIDDPDAPI
                     GTDAMKVLAAIEEVYSDEGVVILIDLGSALLSTEVALEFLDDERRPRVHLCQAPLVEG
                     ALAAAVQASLGAPVEQVMAEAESALAVKIAQMGGRPAGPPPAAPALPAAAEQIVLTVR
                     NRLGLHARPAARLVSTAGRFAADVRLSHNGHDANAKSINQVATLGVRQGDEIAVSAAG
                     PDAAAALAALQALADDNFGDRAEEQGSGGAEEQGAGSPSRPTDLLPADLLNTGLLKGI
                     SASPGVAVGPVALYRPRLPEVVARAIEDTADEWLRLQEAVAAARGEIDALHRQAVGQV
                     GAAEAAIFEAHLLFLQDPALVEATRNRIYGEKINAEAAWQQETAAMAATYRGMADDYM
                     QARAADIEDVAQRVLRRLMGVEPPSLDFAAPAILIAGDLTPSDTARLDRERVLALCTE
                     LGGATAHSAILARALGIPAVVGLGHGVWSVGEGQRVAVDGDAGLLWLRPDDEQVAALA
                     ARREAWLAGQRQAKAAGQGAAVTSDGRRVEIAANVGSPNDVAPALEFGAEGVGLFRTE
                     FLFMGRVAAPTEAEQFDAYARAAAAAGDRPLIIRTLDVGGDKPLPYLDLGEEANPFLG
                     WRAIRFCLERPDVFMPQLRAILRASAGARRNVKLMLPMIGTPAEVRAARAMLRTAQDD
                     LRAAGVPFDEGMEVGIMIEVPSAVAVADQLAREVDFFSIGTNDLTQYVMAADRGNPRV
                     AGLANAFQPSVLRMVRDVVAAAHGRRDHAAGIWVGMCGELAGNPLATPLLVGLGLDEL
                     SMSAPAIPAVKAAVRRVSLADAQRIAAEALALDSAEAIIRYLTDL"
     gene            5413..>5839
                     /locus_tag="PK829_06975"
     CDS             5413..>5839
                     /locus_tag="PK829_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOU40995.1"
                     /translation="MPLEPLEYLAVALAAVAAGAINALAGGGTLITFPALTAMGIPAV
                     SANVTNTVALLPGYLGATFAQKEEVLSQRRRLWLLLPAALGGLVGGLLLLRTDEKLFR
                     QLVPFLILTASLLLAVQEPLRRRLVGKRQPSGGAGEQGSR"
BASE COUNT          862 a         1900 c         2064 g         1013 t
ORIGIN      
        1 cagatgatga tcaagtacgg ctcagagctg gtgccggcgg tgcgcacggc caagttccgc
       61 gcggcctggt cggcggcccg gccgctcatt ggcccgcgcg gggccgacac cggccgcgag
      121 ttgagccgca ccttcaagtg cttcgaccac atcgatgaag gcgttgaggg ctttgtcacc
      181 atcaccggcg gcaaggcgac aaccttgcgc ggcatggccg aaacaacggc caacatcgtt
      241 tgccagaagg tgggcttccc cgtgccctgc ccgacgcgcg actacgtgct gctgccgcat
      301 acggcttact acgtctaatt acgaacgagg aatatagaat gaggaatcga agagagagcg
      361 gcagacggcg gcgcaccccc cgaagcgttc tgaattcctg attcgtaact cctaattcct
      421 aatttctgag agtgccatga cggaacgaga tatcgaattc aaggtctttc gctataagca
      481 gggcggggcc gcgccaacct accagacctt cacctcccgt tgcaacgaga atacgactgt
      541 gctggttgcc ctgcaagaca tccgccgcga ccaggatggc acgctgatcc tgcgccactc
      601 ctgccaccac gccagttgcg gcacgtgcgg catgcgcatt aacggccgcg aggagctggc
      661 ctgcgttgtc aaggtattcg acctgggcac gccgaccgtc gtcgtcgaac ccatgcgcaa
      721 tatgccggtc attagcgacc tggtcgtcga tatggagcca ttctacgacc gtttcaactc
      781 cgccgggatg ccgttcatcc gcgagagcga gtacctggag gaggccgagg tggccgaggg
      841 gttggagacg tacatgcgct gcgagaactg catcgagtgc aacctgtgcg tctcggcctg
      901 cccgatcatg ggcaccgacc cgacctacct cggcccggcg gcgctggccg cggcctggcg
      961 cgtcgtcgag gagccgcgtg ggttggactc gcgtcccttg ctcgactggg tcgatagcca
     1021 gaacggctgc tggcgctgcc acgtggccta tgaatgcaac gaggcttgcc cgtcgggcgt
     1081 cttccccggc gacaagatta tgtcgctgcg acgcgaattg accaaacgca agttccggcg
     1141 gctgttcgga agataattag gaattaagag ttacacacca cagagcactc tgcattccta
     1201 attcgtaatt cctgattact tgggaggaca catggatgac atccagccca caaccgaccc
     1261 tgtgcggccc aaagcgccgc tgcggtcctg gttcgacgtg cgcggccgga gcctgagcac
     1321 gctggccttc gccctcaacc gcctgacggc catcggcctg acggtctacc tgttcctgca
     1381 cctggctgtg ctgagcatgc tgttgggcgg gccggagagc tggaaccggt ttctggccgt
     1441 ggccaggagt ccgttcttct tgctgctcga cgtggtactt ctgttcggcg ttgtctacca
     1501 catggccaac ggcatccgcg tggccctggt gggcatgggc gtcggccaca cgcagcacaa
     1561 gacgatgttc tacgtgctgg tgggtctcgc tctggtgctg ttcgttgtcg gcgcggcgtt
     1621 gatcttcgtc gggtaggagg cgctatcatg caaagctcga tgtcacgacg tacattagag
     1681 gagatggcca cccggcgcga ccgctccggc tcgctctggc tcgtccaggc gtttaccggc
     1741 ctgctgctgg tcttcattct gggcgcgcac atgatcgccc atcatttcat cgtcgagggt
     1801 ggcttgcgcg actaccagca ggtgctggac tacgtggcca acccgttcgt tttcatcatt
     1861 gaagtggtct tcgtcatcat cgccactatc cacgccctgt tgggggcgcg ggccattgtg
     1921 ctcgacctgc ggccgccggc ggctactgtg aaggtcatca actgggtgct ggtggccatc
     1981 ggcgcggtgg ccatcggcta tggcctgtgg ctggctctgg ccttgcaacg ggcggcgggg
     2041 gcgtaatggc caagaaacaa gccgctcccc cgcccccccc acagaccaac tggcgctggt
     2101 actggattcg cacggcgctg atcactgtgc tgatgttcat cttcgtgctg tggtttatgt
     2161 tgccgcgcag tggcagtgtc gcctatctgt gggcgacggt ggccgcggtg gcgatcctgg
     2221 gctacttcgt cgtcagttac ttcatgttga gaagatgacg gcgtcgccgt ccggttgggg
     2281 cagcaatggc gactcggttg gggagtgacg cgacgtggga ggcgcgctgg gcgccctatg
     2341 atgaggagac gtatgcgcgg gcgctggctt atgtgccgcc cggcgcggtc gttcttgaca
     2401 tcggcgcggg cgatctgcgc ttcgcgcggc ggttggcggc gcgggcgcgg ctggtctatg
     2461 ctgtcgagcg gcggccggag ctgttggccg gggcagccgc gtggcctggc gacgtgggca
     2521 acgtgcgcgc cgtctgcgcc gatgcccgcg actggccttt cccgcccagc ctggacgcgg
     2581 ctgtgctgct gatgcgccac tgcgcccacg ctcacttcgc gctctacggc cgcaagctgg
     2641 cggcaacagg ctgccgctgg ctgattacca acgcgcgctg gcgcatgggc gtcgagtgca
     2701 tcgacctgag cgcgccggcg cagccctatg agcgtgtggc tgtgggctgg tacgcctgtc
     2761 gctgtggcgc gaccggcttt cgggctgggc cggtcgaggc gctgactgag gcagccctgg
     2821 ccgaggtgta tgaggttgaa ggttgcccgg agtgcaaaga tggttggtct ggtcttcgtc
     2881 tcccatagcg ctcaactggc cgagggcgtg cttgagttcg ccgggcagat ggcccagggg
     2941 gctgtgcgca tggccgccgc gggtggcatt gacgatcccg acgcccccat tggcaccgac
     3001 gccatgaaag tgctggcggc cattgaagaa gtctacagcg atgagggtgt ggttatcctg
     3061 atcgacctgg gcagcgccct gctgagcacg gaggtggccc tggagttcct ggacgacgag
     3121 cgccggccgc gcgtccacct ttgccaggcg ccactggtgg agggggcgct ggccgcggcg
     3181 gtgcaggcct cgctcggcgc gccggtcgag caggtcatgg ccgaggccga aagcgctctg
     3241 gccgtcaaga tcgcccagat gggcggccgg ccggctgggc cgccgccggc agcgccggcg
     3301 ctgccggccg cggccgaaca gattgtcctg accgtgcgca accggctggg cctgcatgcc
     3361 cgcccggcgg cgcgccttgt cagcacggcc ggccgctttg ccgccgacgt ccggctgagc
     3421 cacaacggcc acgacgccaa cgccaagagc attaaccagg tggccacgct gggcgtgcgc
     3481 cagggagacg agatcgccgt cagcgccgct ggcccggacg ccgccgccgc gctggccgcg
     3541 ctgcaagccc tggccgacga caactttggc gacagggcgg aggagcaggg gagcgggggg
     3601 gccgaggagc agggggcagg aagccccagt cgtccgactg acctactgcc cgctgactta
     3661 ctgaatactg gcttactaaa aggcatctct gcctcgcccg gcgtggccgt tgggccggtg
     3721 gcgctctacc ggccgcggtt gccggaggtg gtcgcccgcg ccatcgagga taccgccgat
     3781 gagtggctgc ggttgcaaga ggctgtggcc gcggctcggg gcgagatcga cgctctgcac
     3841 cggcaggcgg tgggccaggt gggcgcggcc gaggcggcta tctttgaggc ccacctgctg
     3901 ttcctgcaag acccagccct ggttgaggcc acgcgcaacc gcatctacgg cgagaagatc
     3961 aacgccgagg cggcctggca gcaggagaca gcggccatgg ccgccaccta tcgcggcatg
     4021 gccgacgact acatgcaggc ccgcgccgct gacatcgagg acgtggccca gcgggtgctg
     4081 cgccggctca tgggcgtcga accgccgtcg ctcgactttg ccgcgccggc gatcctcatc
     4141 gccggcgacc tgacgccgtc ggacacggcc cgcctcgacc gcgagcgcgt gctggccctc
     4201 tgcaccgagc tgggcggggc caccgcccac agtgccatcc tggcccgcgc gctgggcatt
     4261 cccgcggtcg ttggcctggg gcatggcgtc tggagcgtag gcgaagggca gcgcgtggcc
     4321 gtcgatggcg acgccggctt gctctggctg cggccagacg acgagcaggt ggcggcgctg
     4381 gcggcgcgac gcgaggcgtg gctggccggg cagcgacagg ccaaggccgc cggccagggc
     4441 gcggctgtca ccagcgacgg ccgccgtgtg gagatcgccg ccaacgtcgg cagccccaac
     4501 gacgtcgccc cggcgctgga gttcggcgcg gagggcgtgg gcctgtttcg caccgagttc
     4561 ctgttcatgg gccgcgtcgc cgcgccgacg gaggcggagc agttcgacgc ctatgcccgc
     4621 gccgcggcgg cggccggcga ccggccgctc atcatccgta ccctggacgt tggcggcgac
     4681 aaaccgctgc cctatctcga cctgggcgag gaggccaacc ccttcctggg ctggcgggcc
     4741 attcgcttct gcctggagcg gccggacgtg ttcatgcctc agttgcgggc tatcctgcgc
     4801 gcctcggccg gcgcgcggcg caacgtcaag ctgatgctgc cgatgatcgg cacgccggcc
     4861 gaggtgcgcg ccgcgcgggc catgttgcgc acggcgcagg acgatctgcg cgccgcgggc
     4921 gtacccttcg acgagggcat ggaggtgggc atcatgatcg aggtgccctc ggccgtggcc
     4981 gtggccgacc agttggcgcg cgaggtggac ttcttcagca ttggcaccaa cgacctgacg
     5041 cagtatgtga tggccgccga ccggggcaac ccgcgcgtgg ccgggctggc caacgccttc
     5101 cagccgtcgg tgctgcgcat ggtgcgcgac gtggtcgcgg cggcccacgg ccgccgtgac
     5161 catgccgccg gcatctgggt gggcatgtgc ggcgaactgg ccggcaatcc gctggccacg
     5221 cccctgctgg tcgggctggg gctggacgag ctgagcatga gcgctccggc tatcccggcg
     5281 gtcaaggcgg ccgtgcggcg cgtctcgctg gccgacgccc agcgcatcgc cgccgaagcg
     5341 ctggccctcg actcggccga ggccatcata cgttacttga ccgatctcta agcagcggcg
     5401 ataactcctt tcatgccatt agaaccactc gaatacctgg ccgtcgccct ggctgctgtg
     5461 gccgccgggg ccattaacgc gctggccggc ggcggcacgc tcatcacctt tccggccctg
     5521 acggcgatgg gcatcccggc cgtcagcgcc aacgtcacca acaccgtcgc cctgctgccg
     5581 ggctatctgg gggcgacctt cgcccagaag gaggaggtcc tgtcgcagcg gcggcggctg
     5641 tggttgctgt tgccggcggc gctgggcggc ctggttggcg ggttgctgct gttgcgcacc
     5701 gacgagaagc tctttcgaca gctcgtcccg tttctcattc tgacggcgtc gctgctgctg
     5761 gcggtgcagg aaccgttgcg ccgccggctg gtagggaaga ggcagccgag cgggggagca
     5821 ggggagcagg ggagcaggg
//
LOCUS       DAOSMQ010000167         5834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13844_length_5834_cov_5.263540, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000167 DAOSMQ010000000
VERSION     DAOSMQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13844_length_5834_cov_5.263540"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PK829_06980"
     CDS             complement(<1..303)
                     /locus_tag="PK829_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOU40996.1"
                     /translation="MTLAPELPWARQAIAALAGRGVVVSAGHSLATFDEAAAGFDSGA
                     RYTTHLFNAMPTLHHREPGLAAAALADPRVVVGLIPDGLHVHPALVRLVWQLLGPDR"
     gene            complement(425..796)
                     /locus_tag="PK829_06985"
     CDS             complement(425..796)
                     /locus_tag="PK829_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU40997.1"
                     /translation="MLAIRHATLLTPAATLADATVLVDGARVVAAGGPELAVPAGAAV
                     RDAAGLLLAPGFIDLQVNGGFGLDFTADPETIWDVAAALPRFGVTAFLPTVITSPPAT
                     PQTAQRVLAGGPPAGGPGPAL"
     gene            complement(876..2390)
                     /locus_tag="PK829_06990"
     CDS             complement(876..2390)
                     /locus_tag="PK829_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU40998.1"
                     /translation="MKRIVFGVVLIALVALLVACSPGTAEQLAPTLEAAAEQLAPTVQ
                     AAATELAPTVQAAATELAPTLEAVATEVAPTLEAVATEVSGGETAEGSFLERARAGEF
                     SGTTVSMLGTMVDEEAAKMDAAVAPFEEQTGIDVVYEGSKEFETQINVAVDAGAPPDI
                     ANISQPAMAQRFAAAGELVDISSFLPREKLQETYIDSWLDMATMPGPDGNDIIAGVWH
                     RAAVKSMVFYPKAAFDAAGYEVPETWDEMMALTQQIADDGDTAWCIGIESGAATGWVA
                     TDWMENIMLRTTSLENYDKWVTGELPFASPEVKNAAEIMGDIWLNPDYVYGGVPSIVS
                     TFIGDSPLPMFQDPPGCWFHSQAAWITGFFGDDTLVAGKDYDFFYLPPIDESLGRPAL
                     VAGDMMVMFNDRPEVRALMEFFSKGEGVEQWVRQGGALSPHKDASLDWYENTVDRKVA
                     ELLANATSVRFDASDLMPAEVGSGSFWKAMTDWISGSTDLDTALAEIDASWPQD"
     gene            complement(2477..3427)
                     /locus_tag="PK829_06995"
     CDS             complement(2477..3427)
                     /locus_tag="PK829_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU40999.1"
                     /translation="MVRVGLDIGGTKTAALVVDATGRTLSLVQRPTAVGRPERLVEGI
                     VATLDHALDAAGVARADVAAVGVGVPGQVDPDSGTVKLAVNLNLHEPFPLAGALATAL
                     RTPVTLENDVRAAAWGAFHWASASRPLTSLAYLSIGTGIAAGLVLDGRLYRGAHGMAG
                     EIGHIPVDPAGPRCVCGGYGCLEVFAAGPAIAAQAAAVFPAGTPAPDTQQVYALAATG
                     DPLAGPVVARTATYLARAVYLLVLTYDIECVVLGGGVSRSGGAFERPLRQALAGLRAD
                     SPLAKSMLPDEKIVVLPGDFNAGAVGAVYLADHSSITSRR"
     gene            complement(3427..4602)
                     /locus_tag="PK829_07000"
     CDS             complement(3427..4602)
                     /locus_tag="PK829_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOU41000.1"
                     /translation="MDKATRSQTRHHNSRLVLKTIYDAGEISRAEIARRTRLTRPTVS
                     TLANGLLESGLVVETGPGPSAGGKRPTLLSINEDGPCLLTLDLSGNDYRGALVTLRGR
                     VIRRAAVPAAALRGEAALACVYQLGDKLLPTGPLLGIGVATPGLVDPHRGIVLRAVNR
                     DWTNVPLGQQLAERYECPVHVANDSHMAALAEYTYGDAPDGGNVIVIRVGQGVGAGII
                     LGGRPFYGDGYGAGEIGHVVIDPGGALCSCGNRGCLETTSSIPAMLRAVAAADRRGSP
                     LAGDAPLTWARFAAAVAAHDPVAVDVAVRAGGLLGAAVAQLVGAYNIHHMVLAGRIAD
                     LGDVLLDAAVAEMHQRALPAMAAATTVRYPSLAATQLPDLIALGCSALVLQRELRIL"
     gene            4828..5679
                     /locus_tag="PK829_07005"
     CDS             4828..5679
                     /locus_tag="PK829_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOU41001.1"
                     /translation="MQDNRSIARVGVYVDTANMYRNGGYRMRYDVLREFACRDGAELA
                     RLNAYVSFDADRARSDAVYDKNTHRFYALLRDFGYKVIIKEVHWYEDESGKRYAKADA
                     DLDLAVDMLLQSENLDRVLLVTGDGDFARVVAAVQNRGCRVEVVALDNASTVLRREAD
                     LFLSGYVIPNLVPTVAGGAPVTDAEEWGAFGSIVRGTCYFHKLNYGFMRFLRVMGPRL
                     WLTDTQNPDSPYETAYFHDSNLPDKIDPQQLPSYNHVFQFELAESRHPGGKFEARNIE
                     LLSWIPA"
BASE COUNT          963 a         1941 c         1995 g          935 t
ORIGIN      
        1 ccggtcgggg ccgagcagtt gccacaccag ccgcaccagc gccgggtgga cgtgcaaccc
       61 atcggggatg aggccgacga cgacgcgggg atcagccagc gccgcggcgg ccaggcccgg
      121 ctcgcggtgg tggagggtgg gcatggcgtt gaagaggtgg gtggtatagc gcgcgccgct
      181 gtcgaagccg gcggcggcct cgtcgaaggt cgccagcgag tggccggcgc tgacgacgac
      241 gccccgcccc gccagcgcgg caatggcctg gcgcgcccag ggtagctccg gggccagtgt
      301 caccaggcgt acacccgtcg ccggcgacca gtcccccagt ccggcggccg agggctggcg
      361 caggtaggcc gggttgtggg cgcccttctt gccggggtta agaaacggtc ccccaggtgt
      421 agacctaaag ggcgggcccc ggcccaccgg ccggcggccc accggccagg acgcgctgcg
      481 ctgtctgcgg cgtggccggc ggcgaggtga tgaccgtcgg caggaaggcc gtgacgccga
      541 agcgcggcag ggcggcggcc acatcccaga tggtctccgg gtcggcggtg aagtccagcc
      601 cgaagccgcc gttgacctgc aggtcgatga agcccggcgc gagcagcagc ccggcggcgt
      661 cgcggacggc cgctccggcc gggacggcca actccggccc accggcggcg acgacgcgag
      721 cgccatcgac cagcacagtg gcgtcggcca gagtggccgc gggcgtcagg agggtggcgt
      781 gtcggatagc gagcatggtc gcctcgcgct cttcagccgt agaagaaaac gggccgcacc
      841 cgagtgcggc ccattttcat catggacagt tgtaactaat cctgtggcca ggaggcgtcg
      901 atctcggcca gggcggtgtc caggtcggtc gagccgctga tccagtcggt catggccttc
      961 cagaaggagc cggaacccac ttcggccggc atcaggtcag aagcgtcgaa gcgcacgctg
     1021 gtggcgttgg ccagcaactc ggccaccttg cggtcgacgg tgttctcata ccagtcgagg
     1081 ctggcgtcct tgtggggcga cagcgcgcca ccctggcgga cccactgctc gacgccctcg
     1141 cccttgctga agaactccat cagggcgcgc acttcgggcc ggtcgttgaa catgaccatc
     1201 atgtcgccgg cgaccagggc cgggcggccg aggctctcat caatgggcgg caggtagaaa
     1261 aagtcgtagt ctttgccagc gacgagggtg tcatcgccga agaaaccggt gatccaggcc
     1321 gcctgcgagt ggaaccagca gccgggcgga tcctggaaca tgggcagggg ggagtcgccg
     1381 atgaaggtcg agacgatgga cggcacgccg ccatagacgt agtccgggtt gagccagatg
     1441 tcgcccatga tctcggcggc gttcttcacc tctggcgagg cgaagggcag ttcgccggtg
     1501 acccacttgt cgtagttctc cagcgacgtg gtgcgcagca tgatgttctc catccagtcc
     1561 gtagccaccc aaccggtggc cgcgccggac tcgatgccga tgcaccaggc cgtgtcgccg
     1621 tcgtcggcga tctgctgcgt cagggccatc atctcgtccc acgtctccgg tacttcgtag
     1681 ccggcggcgt cgaaggcggc cttgggatag aagaccatgc tcttgaccgc ggcgcggtgc
     1741 cagacgccgg caatgatgtc attgccatca gggccgggca tggtggccat atcgagccag
     1801 ctgtcgatgt aggtctcttg cagcttctcg cgcggcagga agctgctgat gtccaccagt
     1861 tcgcccgcgg cggcgaagcg ctgggccata gccggttggg agatgttggc gatgtcgggc
     1921 ggcgcgccag cgtcaacggc gacgttgatc tgcgtctcga attccttgga accctcatag
     1981 acgacgtcga taccggtttg ctcctcgaac ggcgctacgg cggcgtccat tttggccgct
     2041 tcctcatcca ccatcgtgcc gagcatgctc acggttgtgc cgctaaactc gccagcgcgg
     2101 gcgcgctcca ggaagctgcc ctcggccgtc tcgccgccag agacttcggt cgccacggcc
     2161 tcaagggttg gggcgacttc ggtggctacg gcttccagag tcggggccag ttcagtggcc
     2221 gccgcctgga cggtcggggc cagctcagtg gccgccgcct ggacggtcgg ggccagttgc
     2281 tcggccgcgg cttccagcgt cggcgcgagc tgctcggccg tgccggggct acaggccacc
     2341 agcagggcga ccagggcgat caggactaca ccaaagacta tgcgtttcat tctttcctcc
     2401 gattgaaatg tgacagactt tctcttgtgc tgctgcaact tgatccactt gggcaagaat
     2461 cgcatcttct gacggttcac ctcctactcg tgatgctgct atggtcggcc aggtaaaccg
     2521 cgccgaccgc gccggcgttg aaatcgccgg gcaaaacgac tattttttcg tcgggtaaca
     2581 tgctcttggc caggggcgaa tcggcccgca ggcccgccag agcctggcgc aacgggcgct
     2641 cgaatgcccc gcccgaccgg gagacgccgc cacccagcac cacacactcg atgtcatagg
     2701 tcagcaccaa caggtagacc gcccgcgcca ggtaggtggc cgtgcgagca acgaccggcc
     2761 cggccagggg gtcgcccgtg gcggccagag catagacctg ctgtgtgtcg ggcgctggcg
     2821 tgccggcggg gaagacggcc gcggcctgcg cggcgatggc cggtccggcg gcgaaaactt
     2881 ccaggcagcc atagccgcca cagacgcagc gcggcccggc cgggtctacg gggatgtggc
     2941 cgatctcgcc ggccatgccg tgcgcgccgc ggtagagccg tccgtctagc accagacccg
     3001 cggcaatacc cgtgccgatg ctcaggtaag ccaggctcgt cagcggccgg ctcgcgctgg
     3061 cccaatggaa tgcgccccag gccgcggcgc ggacgtcgtt ctccagtgtc accggtgtcc
     3121 gcagcgccgt cgccagcgcg ccggccagcg ggaacggctc gtggaggttc aggttaacgg
     3181 ccagtttgac tgtgccgctg tcgggatcca cctggccggg cacgccgacg ccgacggcgg
     3241 ccacgtccgc gcgggctacg ccggcagcgt ccagagcgtg gtcaagcgtg gcgacgatgc
     3301 cctctaccag ccgttcagga cggccaacgg ccgtcggccg ctggacgagg ctgagcgtgc
     3361 gcccggtcgc gtcaacgacc agcgcggccg tcttggtgcc gccgatgtcc aggcccacgc
     3421 gcaccatcac aggatcctca actcgcgctg caacaccagc gccgagcaac ccagggcgat
     3481 caggtcgggc agttgcgtgg cggccagcga cggatagcgc acggttgtgg ccgcggccat
     3541 ggccggcagg gcgcgctggt gcatctcggc cacggcggcg tcgagcaaca cgtcgcccag
     3601 atcggcgatg cgcccggcca acaccatgtg atggatgtta taagcgccga cgagttgggc
     3661 cacggccgcg cccagcaggc cgccggcgcg gacggcgaca tcgacagcca cggggtcgtg
     3721 ggcggccacc gcggcggcga agcgcgccca tgtgagcggc gcgtcaccgg ccaggggcga
     3781 gccgcggcgg tcggccgccg ccaccgcgcg cagcatcgcc gggatgctgc tggttgtctc
     3841 caggcagccg cggttgccgc aactgcacag cgcgccgccg ggatcgatga cgacgtggcc
     3901 aatttcgcct gcgccgtagc cgtcaccgta gaacggccgg ccacccagga taatgcccgc
     3961 gccgacgccc tggccgacgc gaatgacgat gacgttgccg ccgtcggggg cgtcgccata
     4021 ggtgtattcg gccaacgcgg ccatgtggct gtcgttggcc acgtggacgg ggcactcata
     4081 gcgctccgcc agctgttggc ccaggggcac gttggtccag tcgcggttga cggcgcgcag
     4141 gacaataccg cggtggggat cgaccaggcc aggcgtagcc acgccgatgc ccagcagcgg
     4201 gccggtgggc agcagtttgt cgcccaactg gtagacgcag gccagcgccg cctcgccgcg
     4261 cagcgccgcg gcgggcacag ccgcccgccg gatgacgcgg ccgcgcagcg tcaccagcgc
     4321 gccgcggtaa tcgttgccgc tcagatcgag cgtgagcagg caggggccat cttcgttgat
     4381 cgatagcagg gtggggcgct tgccgccggc cgaggggccg gggccggttt cgacgaccag
     4441 accgctctcc agcaggccat tagccagtgt agagacggtc ggccgggtga gccgggtgcg
     4501 gcgggcgatt tcggcgcggc tgatctcgcc agcgtcgtag atcgtcttca ggacaagacg
     4561 actattgtgg tggcgggtct ggctgcgggt cgctttatcc attgtctgag ggcgcaatta
     4621 gtaagttaaa tgaacttaca aagtcaagta tagaacagca gcgcggccct gtcaataggc
     4681 aaagccctgg gacggcgacg ccgataatgc cttgccgctc tctctgcgct ataatgcgcc
     4741 tccggcgggc agcctgatag agcctgaccc atttccctgc cggccggcat catcttattc
     4801 tttaacgtca ctcagaccca aaacacgatg caagacaatc gctccattgc ccgcgtcggc
     4861 gtctacgtcg acactgccaa catgtatcgc aacggcggct accgcatgcg ttatgacgtt
     4921 ttgcgcgaat tcgcctgccg cgacggggcc gaactggccc gtctcaatgc ctacgtcagc
     4981 ttcgacgccg accgggcgcg cagcgacgcc gtctacgaca aaaataccca tcgcttctac
     5041 gccctgctac gcgacttcgg ctacaaggtc attatcaagg aagtccactg gtatgaggac
     5101 gagagtggca agcgctatgc caaagccgac gctgacctcg acctggccgt ggacatgctg
     5161 ctgcaatcgg agaacctcga ccgcgtgctg ctggtcaccg gcgacggcga cttcgcccgc
     5221 gtcgtcgctg ccgtccagaa ccgcggctgc cgggtggagg tcgtcgccct ggacaacgcc
     5281 tcgaccgtgc tgcgtcgcga ggccgacctc tttctgtccg gctacgtcat ccccaacctt
     5341 gtgccgacgg ttgccggcgg cgcgccggtg accgacgccg aagagtgggg ggcgttcggc
     5401 tccatcgtgc gcggcacgtg ttacttccac aagctcaact atggcttcat gcgctttctg
     5461 cgagtcatgg ggccgcgcct gtggctgacc gacacccaga accccgattc gccttacgag
     5521 acggcctact tccacgactc gaatctgccc gacaagatcg acccgcagca attgcccagc
     5581 tacaaccacg tcttccagtt cgagctggcc gagtcacgcc accccggtgg caagttcgag
     5641 gcccgcaaca tcgagttgct cagttggata ccggcctagg gtgcggaaga agcgctcacg
     5701 caaagccgca aagtaagcaa agggcgccag gaggaggctt gattatcggc cgtttcttct
     5761 ttgcgccttt tgccccttgg cgactttgcg tgagcttcta tcgactttac ggtgactctg
     5821 gattgtccgg ccga
//
LOCUS       DAOSMQ010000168         5833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13848_length_5833_cov_4.448771, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000168 DAOSMQ010000000
VERSION     DAOSMQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13848_length_5833_cov_4.448771"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(529..2472)
                     /gene="gyrB"
                     /locus_tag="PK829_07010"
     CDS             complement(529..2472)
                     /gene="gyrB"
                     /locus_tag="PK829_07010"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOU41002.1"
                     /translation="MTSKIKNNEALAATHYDASNIQVLKGLEAVRRRPGMYVGGTDVK
                     AFHHLTNEIVDNSIDEALAGRCDRIEVIIHADGSVSVADNGYGIPVAEHPTEKVSALQ
                     LVHTSLHSGGKFDNAAYKVSGGLHGVGAAAVNALSEWMEVEVKRDGAVWKQRYERGIP
                     MTPVQRLRDLKKGEDTGTTTTFKADETIFVEDHEYRFDMFANRFREMAFVTSGVFIRL
                     SDERVSPAREMSFYFEGGVRSFVRYLNRNRKTLHEPVHVRKELDNVDVEVAVQYTDGV
                     AISEYAFANTINTPDGGTHLTGARTALTRVINNYARKNNFLKEKDKNFSSDDTREGVT
                     LVVSVKHPDPQFESQTKVKLMNAEVAGLVSAVLGEALANFLDENPREAKRIIDRCLTS
                     SRARDAAKAARELVMRKSALESLTLPGKLADCSERDPSRCELYIVEGESAGGTAKQGR
                     DRHFQAILPLRGKILNTERARLDKILGNNEVKALISALGTGIGESFDLGNLRYGRTII
                     MTDADVDGSHIRTLLLTFFFRYMPSLIENGHLYIAQPPLYAVKHGKELLYAYTEGERE
                     TLMKRLNGEKYSVQRYKGLGEMNAEQLWETTMDPNVRTLLQVTIEDAVVADRTFDMLM
                     GDEVAPRRRFITTHASEVRNLDV"
     gene            complement(2476..3378)
                     /locus_tag="PK829_07015"
     CDS             complement(2476..3378)
                     /locus_tag="PK829_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41003.1"
                     /translation="MLLPAALDLFLWLGPRLSFRAFLQDVITTSLAQLPAGVLTIDVA
                     PLMEAAGRINHFRYLSVLLLGLPTLMAGPIPDKTPITPAVIDGGGSGAWLGLLVLFTL
                     VGLLLTAIFYNLIAYALRRSAMTPMPPFGPARFAARTLYTWLRLIALLALLLVLILVV
                     TVPIALLAGFLTLLSQTIGVLVMFGAVVLVLWLVMFLSYTPQGMLLNPRPFFSVVADS
                     VRLFQSNLPTALGLLAVVVVVRQLLSAILLRADSGTWVTAINILAHAYIATALTVALF
                     IFYRDRFVATLRSSSPAGEEPVKQ"
     gene            complement(3527..5596)
                     /locus_tag="PK829_07020"
     CDS             complement(3527..5596)
                     /locus_tag="PK829_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41004.1"
                     /translation="MKPSHRPLLLILVLLLAGCALLPGERGGAPVSLTDATPPPPQPT
                     ATATPAAALGMPQVAVLSALLVDDAHGRLYGTGQVNGLPKLLILDASDGRLLAAWDTP
                     GQLALDAARNRLAVDRGRQGLAVLDATTGEITAEVALPAQDGPPPPQIDATTGTLYAF
                     RESTVYTIDPAIADITHTTTLSVAHSVCGDPAGDAPIARSAYDPAAMRLYLSFITYNC
                     VPWVTTTLVAYDARTMTETGRHDVDIRSQFAPYEGRLFGTSVSRLGPTLYWVWDGASQ
                     WDESSADYSGEPEGMVIDRGRGLIYSALGETLHIINPGDAATNDAAVPLLAGNSLSAH
                     SAGPDTLYFVSPSGRLKLWAAANLFGGQSLPRPAPSALPLGPVVWLALSPNWANDSAM
                     MALVENDTCPGVGNALFILQDTAIGWQPAVLGTPGACEAITAAAFSPTYGRDALLFAA
                     AAEPTTVLRSVDGGRSWVAAETPFPAGVIDQLVVSPAYAADQTLFARLDDGALYRSRD
                     GGQTWRAMSQPLDLLAALDAPGAALTLFGARGGALLRSSDGETWTEIGPTPDGETLLL
                     LAAAPTETPQPVLYAFTAGGRFARSLDGGLSWLTVSEMAATTAQLAIVTAAPPATRPV
                     FMLHYREIMASYDDMASVWASTAVMAAGGYGPTAIALPPNFAAEPYLFVGTVDGQVLR
                     VRADPQP"
BASE COUNT         1005 a         1819 c         2016 g          993 t
ORIGIN      
        1 tttggtgggg gtgtcgtttg gggtgttgtt gctgatcaag ggggtgtttg ggcgggggga
       61 ggagtaaccc acacaggtct cagaacacct gtcaggtcta gcgggccttc cactcttcaa
      121 acgtccagcc ttttatagcc tcgctgaatg gcgttgccgg acgggccaaa cgtcttccgt
      181 ccggcattat gcatccggtg atgtctatga cctgctccag ttgttcgatt gtccagccgg
      241 ccaggaacag gttggccccg ctcaggttgg ccccgcgcac accagcaaga cggcgactat
      301 cgccaggaga atggccgccc accgccggcg cgcccggcgg gcgtcaggta gtgggggcgt
      361 ggaggtgggt gtgtcgttca tcgcttctcg ccagacctcc gaggtttgcc gccaacctca
      421 gaggcctaat aaacaaagaa accggattcc gacgcaagga ctgcgctcag gaaaccggtt
      481 tccaatccct ctaaactcgt cacttgtcgc tcgtcactcg tcactgcact acacgtcgag
      541 gttcctcacc tcgctcgcat gcgtcgtaat aaaccgccgc cgcggggcta cttcgtcgcc
      601 catgagcatg tcgaacgtcc ggtcggcgac gacggcatcc tcgatggtca cttgcagcag
      661 cgtgcgcacg ttggggtcca tcgtcgtctc ccagagctgc tcggcgttca tctcgcccag
      721 acccttgtaa cgctggacgc tatacttctc gccattcagc cgcttcatca gcgtttcgcg
      781 ctcgccctcg gtgtaggcgt agagcagttc cttgccgtgc ttgacggcgt agagcggcgg
      841 ctgggcgatg tagaggtggc cgttctcgat cagcgacggc atgtagcgga agaagaaagt
      901 cagcagcagc gtgcggatgt ggctgccgtc cacgtcggcg tcggtcatga tgatcgtccg
      961 gccgtagcgc aggttgccca ggtcgaacga ctcgccgatg cccgtgccca gggccgagat
     1021 gagcgccttg acctcgttgt tgcccaggat tttgtccagc cgggcgcgct cggtgttgag
     1081 gatcttgccg cgcaggggca ggatagcctg aaagtggcgg tcgcggccct gcttggccgt
     1141 gccgccggcg ctctcgccct cgacgatgta gagttcgcag cgcgatgggt cgcgctcgga
     1201 gcagtcggcc agcttgccgg gcagcgtcag gctttccagc gcgctcttgc gcatgaccag
     1261 ttcgcgggcg gccttggccg cgtcgcgggc gcgcgaggag gtcaggcagc ggtcgatgat
     1321 gcgcttggcc tcgcgcgggt tctcgtccag gaagttggcc agcgcctcgc ccagcacggc
     1381 cgagaccagg ccggccacct cggcgttcat cagcttgacc ttcgtctggc tctcgaactg
     1441 cgggtcgggg tgcttcacgc tgacgacgag ggtcacgccc tcgcgcgtgt cgtcggagga
     1501 gaagttcttg tctttctcct tcaggaaatt gttcttgcga gcgtagttat tgatgacgcg
     1561 cgtcagggcc gtgcgcgcgc cggtcagatg tgtgccgccg tcgggcgtgt tgatggtgtt
     1621 ggcgaaggcg tactcggaga tggctacgcc atcggtgtac tgcacggcca cctcgacatc
     1681 gacgttgtcc aactccttgc gcacgtggac gggttcgtgg agggtcttgc ggttgcggtt
     1741 cagatagcgc acgaacgagc gcacgccgcc ctcgaagtag aagctcatct cgcgcgccgg
     1801 cgagacgcgc tcatcgctca ggcgaatgaa gacgccgctg gtgacgaagg ccatctcgcg
     1861 gaagcgatta gcgaacatgt cgaagcggta ctcgtggtct tcgacgaaga tcgtctcatc
     1921 ggccttaaag gtcgtcgttg tgcccgtgtc ctcgcctttt ttgaggtcgc gcaggcgctg
     1981 gacgggtgtc atgggtatac cgcgctcata gcgctgcttc cagaccgcgc cgtcgcgctt
     2041 aacctcgacc tccatccact ccgacagggc gttgaccgcc gccgcgccga cgccatggag
     2101 gccgccggag accttatacg cggcgttgtc gaacttaccg ccggagtgca ggctggtgtg
     2161 aaccagttgc agggctgata ccttctctgt cgggtgctcg gccacgggga tgccgtagcc
     2221 gttatcggcg acgctgacgc tgccatcggc gtggatgatg acctcaatgc ggtcgcagcg
     2281 cccggccagg gcttcgtcga tggagttatc gacgatctcg ttggtcaggt ggtggaacgc
     2341 cttcacgtct gtgcccccga cgtacatgcc cgggcggcgg cgcaccgctt ccagaccctt
     2401 gagtacctga atgttgctgg cgtcgtagtg ggtggctgcc agggcctcgt tgttcttgat
     2461 cttactggtc ataggctact gtttcacagg ttcctcgccc gccggcgagc ttgaacgcag
     2521 agtggcgaca aagcggtcgc gatagaaaat gaatagggcc acggtcagcg ccgtggcgat
     2581 ataggcatga gccaggatat tgatcgccgt cacccaggtg cctgaatcgg cgcgcagcaa
     2641 aatggcgctg agcagctgtc gcacgacaac cacgacagcc agcagcccca aagcggtcgg
     2701 caggttgctc tggaagagac gcacgctgtc ggccactacg ctaaagaagg ggcgcgggtt
     2761 tagcagcatt ccctgaggcg tgtagctgag gaacatgacc agccacagca caagcacgac
     2821 cgcgccgaac atcaccaaca cgccgatcgt ctggctcagg agcgtcagga agccggccag
     2881 cagggcgata gggacggtaa cgaccagaat gagcaccagc agcagggcca gcagggctat
     2941 gagccgcagc caggtgtaga gggtgcgggc ggcgaaacgc gccgggccga agggcggcat
     3001 gggcgtcatc gcggagcgcc gcagagcata ggcgatcagg ttgtagaaga tcgccgtcag
     3061 cagcaatccc accagcgtaa acaacaccag cagccccagc caggcccccg agccgccgcc
     3121 gtcgatgacc gccggggtga tgggcgtctt gtccgggatc ggcccagcca tcaacgtcgg
     3181 caggccaagc aacaacacgc taaggtagcg gaagtggttg atgcgcccgg ccgcctccat
     3241 gagcggggca acgtcgatgg tcagcacgcc cgccggcaac tgcgccaggc ttgtcgtaat
     3301 gacatcctga aggaaggcgc gaaagctcaa ccgcggcccg agccagagaa agagatcaag
     3361 agcggcgggt aacagcatca gccataggta acgagtggtc aactcgaacc cggccgacaa
     3421 cgtggaaaaa acgcccggaa gcgtggtggt ttggccctgc ttttgcacca tgatagcaga
     3481 ccaattatat ctcatttgtt ctatctgggc acgcggacgc tctctcttac ggctgcgggt
     3541 cggcgcggac gcgaagcacc tgaccatcga ccgtgcccac gaacaggtac ggctcggcag
     3601 cgaagttggg cggcagagcg atggccgtcg ggccatagcc gccggcggcc ataaccgcgg
     3661 tcgaggccca gacgctggcc atgtcgtcgt aggaggccat gatctcgcgg tagtggagca
     3721 tgaagacggg gcgcgtggcc ggcggcgcgg ccgtgacaat ggccagttgc gccgtcgtgg
     3781 ccgccatctc ggacacggtc agccatgaca acccgccatc gagcgaccgc gcaaagcggc
     3841 ccccggccgt gaaggcatag agcactggtt gtggcgtctc ggttggcgcg gcggccaaca
     3901 gcagtagcgt ctcgccatcg ggcgtcggcc caatctccgt ccatgtttcg ccgtcgctcg
     3961 aacgcagtag cgcgccgccg cgcgcaccga acagcgtcag cgccgcgccc ggcgcgtcga
     4021 gcgccgccag caggtcgagc ggctggctca tggctcgcca ggtctgccca ccatcgcgcg
     4081 agcggtagag cgcgccatca tccaggcggg cgaagagggt ctgatcggcc gcataggccg
     4141 gcgatacgac cagctgatca atgaccccgg ccgggaaggg cgtttccgcc gccacccagg
     4201 agcgcccgcc gtccaccgag cgcaggacgg tggttggctc ggccgcggca gcgaacagca
     4261 gcgcgtcgcg gccataggtg ggcgagaaag ctgccgcggt gatggcttcg cacgcgcccg
     4321 gcgtgcccag caccgccggc tgccagccta tggccgtgtc ttgcaggatg aacagcgcat
     4381 tgccgacgcc cggacaggtg tcgttttcca ccagggccat catggcgctg tcgttggccc
     4441 agttgggcga cagagccagc cagacaaccg gcccgagggg cagcgccgag ggggccgggc
     4501 ggggcagcga ctggccaccg aacagattgg ccgccgccca gagcttcagg cggccggagg
     4561 ggctgacaaa gtagagcgta tccgggccgg cgctgtgggc gctcagcgag ttgccggcca
     4621 gcaggggcac agccgcgtcg ttggtggccg cgtcgccggg gttgatgatg tgcagcgtct
     4681 cgcccagggc cgagtagatt agcccgcgtc ccctgtcgat gaccatcccc tcgggctcgc
     4741 cgctgtagtc ggcgctgctc tcgtcccact gcgacgcgcc gtcccagacc cagtacagcg
     4801 tcgggccgag gcgcgacacc gacgtgccga agaggcgacc ctcatagggc gcgaactgcg
     4861 agcggatgtc gacgtcgtgg cggcccgtct cggtcatggt gcgcgcgtcg taggccacca
     4921 gagtcgttgt cacccagggc acgcagttgt aggtaatgaa cgacaggtag aggcgcatcg
     4981 ccgcggggtc ataggcgctg cgggcgatgg gcgcgtcgcc ggccgggtcg ccacagacgc
     5041 tgtgggcgac gctgagggtt gtggtatgag tgatgtcggc gatggccggg tcaatagtgt
     5101 agacggtgct ctcgcggaag gcgtagagcg tgcccgtggt ggcgtcgatc tgcggcggcg
     5161 gcggcccgtc ctgggcgggc agggccacct cggccgtgat ctcgcccgtg gtcgcgtcga
     5221 gcacggccag cccctgccgg ccgcggtcga cggccagccg gttgcgggcc gcgtccagcg
     5281 ccagttgacc gggggtgtcc catgcggcca gcaggcggcc gtcgcttgcg tcgaggatga
     5341 gcagcttggg caggccgttg acctggccgg tgccgtagag gcggccgtgg gcgtcgtcga
     5401 ccagcagcgc cgagaggacg gctacctgag gcatccccaa tgccgccgcg ggggtggcgg
     5461 tggcggtggg ctgcggggga ggcggcgtcg cgtctgtcag cgaaacgggg gcgccgcccc
     5521 gctcgcccgg cagcaaggcg cagccggcca ggagcagcac caggatgagg aggagagggc
     5581 gatggctggg cttcatggaa acagtatagc ggaaaacaag gccggccgat cgtcgccggg
     5641 ccaccgtttg cctgccggcc ggcccacgcc caggtaggcc aatagaaagg gaggccgccc
     5701 tcgtgggcac agcttttcag ttgtcaccaa ttgagtcgta cagcctgtca ggctgtcgtc
     5761 gccggccaca gcgacagcca cagcctgtta ggacattaac aaattaacag gcattttggt
     5821 taagctcctc agc
//
LOCUS       DAOSMQ010000169         5823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13877_length_5823_cov_4.450763, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000169 DAOSMQ010000000
VERSION     DAOSMQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13877_length_5823_cov_4.450763"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..458)
                     /locus_tag="PK829_07025"
     CDS             complement(66..458)
                     /locus_tag="PK829_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41005.1"
                     /translation="MTMYTGDDPDHGRADPNVVRRLLQHVPLSVTQTVLITRGPQLLA
                     HRGALSADEAGEVAVFVAEGWRDAGQTLRIQYMPVPLRSTARLLLTYPLRDGYQMTLA
                     DAEAAPLEPLRRLGGQLIAVLAAAGIGR"
     gene            complement(517..2463)
                     /locus_tag="PK829_07030"
     CDS             complement(517..2463)
                     /locus_tag="PK829_07030"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41006.1"
                     /translation="MRRVYLLLVFGWLLAMQAPALTIGAIQGGAAESPYLNRFVTFRG
                     VVVGRYEDENTRGDVYYTLFVQSLAGQTDGDPATSDGIAVFLGREPRRDVALGDVVRV
                     GGKVTEFYGLTEIDDRGLTVTVEDRIPLPAPIALDPPADNDTAAAVLEALEGMRVSLG
                     EAVVSGPTHTGCGFAVVSALSATSLPLLRRADDDPTGQAVPVLYPSDLNCDDIPQVTT
                     GDRIDGIAGALTYNFDQFKIVLDGADELEITPSPRPAVPAPPTLRGQQFSVATLNAED
                     YFDAVRDTADDGEPLPTAEEVAARQAKLSAQIAGPLGCPTLLALQEVEHEALLRDLAA
                     AVATACGFTYAVSHHDSPDARGIDTALLSDPRRVRVGAVALRQTCSPVPTGISDASIT
                     CPPGKEPLFGRPPLEVEATIDGQPAIIWVNHFKSKRDGEIDTALARIQQAVYLNGLAG
                     ERLAADAGFRLIALGDFNDTELSPALTLLTGPAESGHFANALSAVPPAQRYTYNFGGV
                     SELIDGILLSPALAAEVSAATIVHLNTDYPAGLRLDTAPERLPFRASDHDPALVVLGR
                     APATPTPAPLPTATAAPAPTATAVTPPTPRPTPAPAPSPTPAPAALPSPATRGPGPAV
                     IAAGVSTLALVAVAGLIALRRGRR"
     gene            2678..5098
                     /gene="lon"
                     /locus_tag="PK829_07035"
     CDS             2678..5098
                     /gene="lon"
                     /locus_tag="PK829_07035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOU41007.1"
                     /translation="MSNTMSDYHESKPIPVVPLRGGAVFPGVTTTVSIGRRTSLAAVQ
                     AAIERGGDLLVVVQRDAEVETPGTDDIFPIAILATARDMLRTPVGIQMLVELHRRVRL
                     LGVESGGSFLIGRYEELAEEDAVDETLKLEAIAYLEQYVELMGETNQQIITMTRASRT
                     AGALADAIAGLITLPFEAETALLMNLNGRARLEAVLEHLRQEVRIADIRSKIQQDARD
                     GAEKAQRDYMLREQMKAIRKELGDEDGDGPDSLRDKIEAAGMPPKVLERALSELKRLE
                     YQGGQSAEASVIRTYLEWLTELPWNNETEDNLDIVHVRQVLDEDHYGLEDVKERIVEY
                     VAVRKLAGKDMKGAIINLNGPPGVGKTSIATSVARAMGREMVRISLGGVRDEAEIRGH
                     RRTYIGAIPGHIIRALRDAKTRNPVIVLDEIDKVGNDWRGDPAAALLEVLDPEQNHSF
                     TDHYLEVPFDLSQVIFITTSNQLGTIPAALLDRMETITMPGYIEDEKAAIARQYLLKK
                     QQVANGIGGVTVTLADDALRSLIRYYTLEAGVRQLERNLGKVVRKLAVKVASGETGPF
                     TVGEADVEEIMGPRKFSYGMMEDQDEVGLVTGLAVNNYGGNTLPIEVSLSEGTGRITL
                     TGSLGDVMQESAQAALTYARANARKLGLEPGLFDKVNVHVHVPDGATPKDGPSAGIAM
                     ATAIISAFTRRPVRRDVAMTGEVTLRGKVLPIGGLKSKTIAAHRAGIKTVLLPKDNAK
                     DIPELPERVRQDLELIPVGHLDEVFAVALLEPVGPPMKIGEEKPVEPVVVPPPINGAN
                     GERVGRVG"
     gene            complement(5332..>5823)
                     /locus_tag="PK829_07040"
     CDS             complement(5332..>5823)
                     /locus_tag="PK829_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU41008.1"
                     /translation="EAQARHEAEAKAKQAAEAKARQEADAKAKAEAEARLKKAREEAE
                     ARQQKLEAERRLEKAREEAEARQKEALAKREAARAEEERAAAEAAAAKEAAAKEAAAA
                     PRFTEFIAEHTVVAGDNLSFISQKYYGTQANFRLIYEANKDVIGDNMNIIRPGQKLRI
                     PKL"
BASE COUNT          963 a         1920 c         2027 g          913 t
ORIGIN      
        1 acgcgatgga tattctaccc agacgacggg cagaaagcaa acggaaacgc cggtggcgcg
       61 gccgcctacc ggccgatgcc cgccgccgcc aggacggcga tcagctgccc gcccagccgc
      121 cgcagcggct ccagcggcgc ggcctcggcg tcggccagtg tcatctggta gccgtcgcgc
      181 agcgggtagg tcagcagcag ccgggccgtg ctgcgcaggg gcacgggcat atactggatg
      241 cgcaacgtct gccccgcgtc gcgccagccc tcggccacga agacggccac ctcacccgcc
      301 tcgtcggcac tcagcgcgcc acggtgggcc agcaactgtg gcccccgtgt gatcagtacc
      361 gtctgcgtga cgctcagcgg cacgtgttgc aacagccggc gcacgacgtt ggggtcggcg
      421 cggccgtggt cgggatcgtc gcctgtgtac atggtcatcc tgcctctatc ctaccccgcc
      481 tcgcgcgccg tgccatgcag gaagcgtaaa agtagcttag cgccggccgc gccgcagggc
      541 gatcaggccg gcgacggcga ccagcgccag ggtggagacg ccggcggcga tgacggccgg
      601 gccaggcccg cgcgtcgccg gtgacggcag agccgccggt gccggtgtcg gcgagggcgc
      661 gggtgccggc gttggccggg gcgttggcgg tgtgacggcc gtggcggttg gcgcaggggc
      721 ggctgtcgcc gtgggcagcg gcgcgggtgt cggcgtggcc ggggcgcggc cgaggacgac
      781 cagcgccggg tcgtggtcgc tggcccggaa gggcaggcgc tccggcgcgg tgtccagccg
      841 caggccggcg gggtagtcgg tgttaaggtg gacgatggtc gccgcgctga cctcggccgc
      901 cagcgccggc gacagcagaa tgccgtcgat caactcgctc acgccgccga agttgtaggt
      961 gtagcgttgg gccggcggaa cggcgctcag ggcgttggcg aagtggccgc tctcggccgg
     1021 gccggtcagc agggtcagcg ccggcgacag ctccgtgtcg ttgaagtcgc ccagggcgat
     1081 cagacggaag ccggcatcgg ccgccaggcg ctcgcccgcc agcccattca ggtagaccgc
     1141 ctgctggatg cgcgccaggg cggtgtcgat ctcgccgtcg cgcttcgact tgaagtggtt
     1201 gacccagatg atcgccggct gcccgtcgat agtcgcctcg acctccagcg gcgggcggcc
     1261 gaagagcggc tccttgcccg gcgggcaggt gatgctggcg tcgctgatgc cggtgggtac
     1321 aggcgagcac gtctgccgca gggccaccgc gccgacgcgc acccgccgcg gatcgctcag
     1381 cagggcggtg tcgatgccgc gggcgtcggg gctgtcgtgg tggctgacgg cataggtgaa
     1441 gccgcaagcc gtagccactg ccgcggccag gtcgcgcagc aatgcctcgt gctccacctc
     1501 ttgcaacgcc agcagcgtcg ggcagcccag cggcccggcg atctgggcgc tgagcttggc
     1561 ctgccgcgcg gccacctcct cggccgttgg cagcggttcg ccgtcgtcgg ccgtgtcgcg
     1621 gacggcgtcg aaataatcct cggcgttgag cgtggccacg ctgaactgct gcccccggag
     1681 cgtgggcggc gccggcacgg ccggccgcgg cgacggcgtg atctccagtt cgtccgcgcc
     1741 gtcgaggacg atcttgaact ggtcgaagtt gtaggtcagc gccccggcga tgccgtcgat
     1801 gcggtcgccg gtcgtcacct gggggatgtc gtcacagttg aggtcgctag ggtagaggac
     1861 aggtacggcc tggccggtgg ggtcgtcgtc ggcgcggcgg aggagtggca gggacgttgc
     1921 ggatagcgcg ctgacaacgg cgaagccgca gcccgtatgc gtcggcccgg acacgaccgc
     1981 ctcgcccagt gacacgcgca tgccctccag cgcctcgaga acggccgccg cggtgtcgtt
     2041 gtcggccggt ggatcaaggg caatgggcgc gggcaggggg atgcggtctt cgacggtcac
     2101 ggtcaggccg cggtcgtcga tctcggtcag gccgtagaac tcggtcacct tgccaccaac
     2161 gcgcacgacg tcgcccaacg ccacgtcgcg ccgcggctcg cgccccagga agacggcgat
     2221 gccgtcggag gtggccgggt cgccatcggt ctggccggcc aggctctgga cgaaaagggt
     2281 gtagtaaacg tcgccgcggg tgttctcgtc ctcatagcgg ccgacgacca cgccgcggaa
     2341 ggtgacgaag cgattgagat aaggggactc agccgcgcca ccctggatcg cgccgatagt
     2401 cagcgccggc gcttgcatgg ccagcagcca gccaaacacc aggagcaggt agactcgtcg
     2461 catggcttac cgttaccctc gttcaaaccc cccggccgcc tccagacaag gccagccagt
     2521 ggaattgtcg cccaaacgtg tgggcaggca agagatgtca ttcttataca tctctgacgc
     2581 gcgtctatca taaaacatat tcccgtcgcg tagaatctgc tgactgccac tctatctcgt
     2641 ggcagccaag aagggactca gtattgagga taattctgtg agtaatacga tgagcgacta
     2701 tcacgaaagc aaacccatcc ccgtcgtgcc tttgcgcggc ggcgcggttt ttcccggcgt
     2761 cacgacgacc gtatccatcg gccggcggac gtccctggcc gcggtgcagg cggccatcga
     2821 acggggcggc gacctgttgg tcgtcgtgca gcgcgatgcc gaggtggaga cgcccggcac
     2881 cgatgacatc ttccccattg ccattctggc cacggcgcgc gacatgctgc gcacgccggt
     2941 cggcatccaa atgctggtcg agttgcaccg gcgcgtgcgc ctgctgggcg tcgagtcggg
     3001 cggctcgttc ctgatcggcc gctacgaaga actggccgaa gaggacgccg tcgacgagac
     3061 cctcaaactg gaagccattg cctatctgga gcaatacgtc gagctaatgg gcgagaccaa
     3121 ccagcagatc attaccatga cccgcgccag ccggacggcc ggcgcgctgg cggatgctat
     3181 cgctggactg attaccctgc cgttcgaggc cgagacggcc ctgctaatga acctcaacgg
     3241 ccgggcgcgg ctcgaagcgg ttctggagca tctgcgccag gaagtccgca ttgccgacat
     3301 ccgcagcaag attcagcagg atgcccgcga cggcgcggaa aaggcccagc gcgactacat
     3361 gctgcgcgag cagatgaagg ccatccgcaa ggaactgggc gacgaggacg gcgacggccc
     3421 cgacagcctg cgcgacaaga tcgaggccgc cggcatgccg ccgaaggtgc tggagcgggc
     3481 gctgagcgag ctgaagcgcc tggagtacca gggcgggcag tcggccgagg ccagcgtcat
     3541 ccgcacctat ctggagtggc tgaccgagct accgtggaac aacgagaccg aggacaacct
     3601 cgacatcgtc cacgtccgcc aggtgctgga cgaagaccac tatggtctgg aggatgtgaa
     3661 ggagcgcatc gtcgaatacg tcgccgtgcg caagctggcc ggcaaggaca tgaagggcgc
     3721 gatcatcaac ctcaacggcc cgcccggcgt cggcaagacg tcgatcgcca cctcggtggc
     3781 gcgggcgatg gggcgtgaga tggtgcgtat cagcctgggc ggcgtgcgcg acgaggccga
     3841 gattcgcggc caccgccgca cctacatcgg ggcgatcccc gggcacatca tccgcgccct
     3901 gcgcgacgcc aagacgcgca acccggtcat cgtcctggac gaaatcgaca aggttggcaa
     3961 cgactggcgt ggcgaccccg ccgcggcgct gctggaagta ctcgacccgg agcagaacca
     4021 cagcttcacc gaccactacc tggaagtgcc atttgacctg agccaggtga tcttcatcac
     4081 cacgtcgaac cagctcggca ccatcccggc ggcgctgctc gaccgcatgg agacgatcac
     4141 catgcccggc tacatcgagg acgagaaggc ggccatcgcc cggcagtacc tgctgaagaa
     4201 gcaacaggtg gccaatggca tcggcggcgt gaccgtcacg ctggccgacg acgccctgcg
     4261 ttcgcttatc cgctactaca cgctggaagc gggcgtgcgc caactggagc gcaacctcgg
     4321 caaggtcgtg cgcaagctgg ccgtcaaggt ggcctccggc gagactgggc cgttcaccgt
     4381 gggcgaggcc gacgtagaag agatcatggg gccgcgcaag ttcagctacg gcatgatgga
     4441 agaccaggac gaggtcggtc tggtgacggg ccttgcagtc aataactatg gcggcaacac
     4501 cctgcccatc gaggtcagcc tgagcgaagg cactggccgg atcacgctga ccggctcgct
     4561 gggcgacgtg atgcaggagt cggctcaggc agcgctgacc tacgcgcggg ccaacgcccg
     4621 gaagctgggt ctggagccgg ggctgtttga caaggtcaac gtccacgtgc acgtgcccga
     4681 cggggccacg cccaaggacg gccccagcgc gggcattgcc atggcgacgg cgatcatctc
     4741 cgctttcacc cggcggccgg tgcgacgcga cgtggccatg accggcgagg tgacgctgcg
     4801 cggcaaggtg ctgcctatcg gtggcctgaa gtccaagacc atcgccgccc accgcgcggg
     4861 catcaagacc gtgctgctgc cgaaggacaa cgccaaggac atcccggaac tgccggagcg
     4921 tgtgcggcaa gacctggagc tgatcccggt cggccacctg gacgaggtct tcgccgtggc
     4981 cctgttggag ccagtggggc cgccgatgaa gatcggcgaa gagaagccgg ttgagccggt
     5041 tgtcgtaccg cccccgatca atggagccaa cggggagcga gtcggccgcg tggggtaaga
     5101 agtagctgct ttcagttgaa gtgccgctat cgaatctgat agcggcactt ttttattcat
     5161 cccattggca acaatcgcac gctgcgcccg ccccgccagc ggcacggtcg cggctggctt
     5221 gtggctggct tgtagctctc ttgggcaccg aaaccaccag ccagcgcgct aactgagggc
     5281 gctcctcatt agacagcggt actattctgt tgccgtgtct gtgcaacgat cctacagctt
     5341 cggaatccgc aacttctgcc ccggccgaat gatattcatg ttgtcgccga tgacgtcctt
     5401 attggcctca tagatcaggc ggaagttggc ctgcgtgccg tagtactttt gggaaatgaa
     5461 gctcaggtta tcaccggcaa cgacagtatg ctcggcgatg aactccgtga agcgcggcgc
     5521 ggcggcggct tccttagcgg cagcctcctt cgcggcggcg gcttcggctg cggcgcgctc
     5581 ctcttcggcc ctggcggctt cgcgcttggc cagggcttcc ttctgacggg cctcagcctc
     5641 ctcgcgcgcc ttctccaacc gccgctcggc ctccagcttt tgctggcgcg cctcggcctc
     5701 ttcgcgggcc ttcttcaggc gcgcctcggc ctcggccttc gccttggcgt cggcttcctg
     5761 ccgcgccttg gcctcggccg cttgcttcgc cttggcctcg gcctcatgcc gggcctgggc
     5821 ttc
//
LOCUS       DAOSMQ010000170         5807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_13939_length_5807_cov_4.619263, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000170 DAOSMQ010000000
VERSION     DAOSMQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13939_length_5807_cov_4.619263"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1125)
                     /locus_tag="PK829_07045"
     CDS             complement(202..1125)
                     /locus_tag="PK829_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOU41009.1"
                     /translation="MIPVDPRGCAGKIIDIHGQKGMAWLAALPALVAEVAARWSLTVR
                     PPFPELSYNYVAPVVGPGGEAWVLKAGVPNRELTDEAAALRLFDGRGIARLIDADTER
                     GVLLLERVLPGEPLKSVADDGTRVRTVAGLIGELPRPLPTDGSLATGHPFRTIAHLAR
                     GLDRLRQMFGGGYGPFPARRVERAAALFAGLAGGRPQLIHGDMNPGNVLRAMRAPWLA
                     IDPKGYAGDPLFDVATFLNDPPAGLAADDLRRLQARRVALFAEVLGQPRAAVLAWAEA
                     HAVLSGWWSYEDHGRGWEKGLALAALYEALG"
     gene            1124..1205
                     /locus_tag="PK829_07050"
                     /pseudo
     CDS             1124..1205
                     /locus_tag="PK829_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1252..1542
                     /locus_tag="PK829_07055"
     CDS             1252..1542
                     /locus_tag="PK829_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOU41010.1"
                     /translation="MAAWRHPQYSAAALDDVFEELRTWRWLRELRTVNHYHAHPGYFA
                     ALAASICEVWAAGGRPDRLLMSFHGIPRDYFLNRPDPLAGLAEPPARCRSGR"
     gene            complement(1520..2629)
                     /locus_tag="PK829_07060"
     CDS             complement(1520..2629)
                     /locus_tag="PK829_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU41011.1"
                     /translation="MPEITRVGIIGCGNISSAYINGCRAFDFLRVTGVADLRPEVAQA
                     KAAEHDLACAGSVEALLADPDIDIVINLTVPSVHASVSLAILAAGKHAYSEKPLAVER
                     ADGRAVLDAAAGQGLRVGCAPDTFLGGGLQTCRRLIDEGAIGEPVAATAFFLGHGPES
                     WHPNPGFFYRAGAGPLFDMAPYYLTALVHLLGPVAQVAALARISFAERLATSAARQGE
                     RLPVEVPTHVAGTLAMSAGPLATMITSFDVWGHSLPRIEIYGSEGSLSVPDPNTFGGP
                     VRLLRPGGEWEDVPLSHSDAVRRGVGVADMALAIAEGRPHRATGEMAYHVLDVMHSFY
                     DSSAEGRTVSVDSTCVQPAAMPAGAPVGFTGLIGI"
     gene            complement(2622..3389)
                     /locus_tag="PK829_07065"
     CDS             complement(2622..3389)
                     /locus_tag="PK829_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU41012.1"
                     /translation="MTKPIALQLYSLREALARDFEGTIRAVAAIGYAGVETAGFPDGV
                     SPTAAKALFDELGLVICSAHAPLPLGDAAATTLAQLQALGCERLVCAWLPPETYATLD
                     STYRAIDQLNEAASVCAGHGLRLGVHNHWFEFEPLAGTGQRPYEVWLERLEPHIFFEL
                     DAYWARVGGVAPLEALRRMGPRVELLHVKDGPADGTQSPMTAVGTGALDYTTIIPAAT
                     AAEWLIVEIDRCATDMLTAVEASQRYLVGKGLGHGHA"
     gene            3535..4233
                     /locus_tag="PK829_07070"
     CDS             3535..4233
                     /locus_tag="PK829_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41013.1"
                     /translation="MFRAARLTLIFAVLFAFFLIAPAFLNQPFGPYPLLRVGNILDLL
                     TPLVLIPVYWLLFQVAPDRRITDRETLVFLVLAGLWVMGHSLHLAANAIGHLNEGLAG
                     DAPALTHFLDEDLSHTIWLLGILGLAALILYRQWRHPFAGPRQGNGLVLAAAAIHGFN
                     YFLSIVEAGTNGLGIGFAVTVVVFGLLQRRELGQRPITRFFVFAYAISLVFFLAWGLY
                     WGGLPEFSQVGLLN"
     gene            4251..5291
                     /locus_tag="PK829_07075"
     CDS             4251..5291
                     /locus_tag="PK829_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOU41014.1"
                     /translation="MLDQSPLSDDTLLACARERFGRPAVAITFLPLGYDANAWAYRLE
                     ADKAYFLKVRRGPAALPGLLAPRAVADAGIDGVLTPLPTTDGALWAALGDFTVTLQPF
                     VAGRDAWDSGLSDAQWLAFGNLLRRVHALPVPPALAAHLVLERFVPDDMAFMPALAAH
                     AHGAAHVHGPRPDDPLAAGLAACWRQHETTIATLQHQAAVLGEQLRRADPPRVLCHAD
                     AYLGNVLVGDDGRLWLIDWDGAALAPRERDLMFVFGGIAAGQAGPRETALFVRGYGPV
                     APDAAALTYYRCAWVLQDLASFGHTVLDAAAGEATRREALRLFKSNFAPGELAALALS
                     RVATTIEGDHVS"
     gene            5281..5706
                     /locus_tag="PK829_07080"
     CDS             5281..5706
                     /locus_tag="PK829_07080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HOU41015.1"
                     /translation="MFLDLNQPKHAHVDARLRAESILWLGTVRPDGRPHLVPVWFLWD
                     GESISIYSQPDAQKLRNLAANPAVTVALEALDEGSDVAIIEGEARVGAAADAPAVAAA
                     YTDKYAAAIADLGYTPEGMLGAYSRLIRVTPRRLLAWSG"
BASE COUNT          821 a         2125 c         1952 g          909 t
ORIGIN      
        1 ccgtcggcta tgagccgcgc accgtgcccg actggggcgg cccggcggct gataccttcg
       61 ctgccctgcg cctggagccg tggcggctgc gcgtcttccc cggcagcgtc ctgatgggcc
      121 agggtggcac gatcctgacc tggcaggaga gcggctaagc gcggcgaagc cgtccacgcg
      181 gtcgccggcg gcgcggccgg cctagcccaa cgcctcgtag agcgccgcca gcgccagccc
      241 cttctcccag ccgcggccgt gatcctcata gctccaccag ccagagagca cggcgtgggc
      301 ctcggcccag gctaggacag ccgcgcgcgg ctgccccagc acttcggcga acagggccac
      361 gcgccgcgcc tgcaagcggc gcaggtcgtc cgcggccagc ccggccgggg ggtcgttgag
      421 gaaggtggcc acgtcgaaga gcgggtcgcc ggcgtagccc ttggggtcga tggccagcca
      481 gggcgcgcgc atggcccgca ggacgttgcc ggggttcatg tcgccgtgga tgagctgtgg
      541 ccgtccgccg gccagcccgg cgaacagcgc cgctgcccgt tccacgcgcc gcgccgggaa
      601 ggggccatag ccgccgccaa acatctgccg cagccggtcg aggccgcgag ccaggtgggc
      661 gatggtgcgg aagggatggc cggtcgccag cgacccgtcg gtgggcagcg gccgaggcag
      721 ctcgccgatg aggccagcca ctgttcgcac gcgcgtgccg tcgtcggcca cgctcttgag
      781 cggctcgccg ggcaggacgc gctccagcaa caggacgccg cgctccgtgt cggcgtcgat
      841 gaggcgggcg atgccgcggc cgtcgaacag ccgcaacgcc gcagcctcgt cggtcagttc
      901 ccggttgggc acgcccgcct tcagcaccca cgcctcgccg cccggcccga cgaccggggc
      961 gacgtagtta taggacaact ccgggaaggg cggccggacg gtcagagacc agcgcgcggc
     1021 cacctcggcc accagcgccg gcagcgccgc cagccaggcc atgccctttt ggccgtgaat
     1081 gtcgatgatc ttgcccgcgc agccgcgggg gtcaacggga atcatggccg tcattgtagc
     1141 tgccaatgtc gggtcgccgg acgaaccaac cgcgcccgcc ctgcgccgct atctggctca
     1201 attcctgggc gacccgcgcc tcatcgagta cccgcgctgg cggtggcagc cgtggctgca
     1261 tggcgtcatc ctcaatactc ggccgcggcc ctcgacgatg tcttcgagga actgcgaacg
     1321 tggcgctggc tgcgggagtt gcgcaccgtc aaccactacc acgctcaccc cggctatttc
     1381 gccgcgctgg ctgcctctat ctgtgaggtg tgggcggccg gcggccggcc cgaccggctg
     1441 ctgatgtcgt tccacggcat cccgcgcgac tacttcctta atcgccctga cccacttgca
     1501 gggctggctg agccgcccgc tagatgccga tcaggccggt gaagcccacc ggcgcgccgg
     1561 cgggcatggc cgccggctgg acacaggtgc tgtccacgga tactgtccgc ccctcggccg
     1621 acgagtcgta gaagctgtgc atcacgtcga gcacgtggta ggccatctcg cccgtggcgc
     1681 ggtggggccg cccctcggca atggccaggg ccatatcggc cacgcctacg ccccggcgca
     1741 ccgcgtcgct gtggctcagc ggcacgtcct cccactcgcc gcccggccgc agcaaccgca
     1801 ccggcccgcc gaaggtgttg gggtcgggca cgctcagcga cccctccgag ccgtagattt
     1861 cgatgcgcgg cagtgaatgg ccccagacgt cgaagctggt gatcatcgtc gccagcgggc
     1921 cggcgctcat ggccagcgtg ccggccacgt gggtcggcac ttccaccggc agccgctcgc
     1981 cttgccgcgc cgcgctggtg gccaggcgct cggcaaagga gatgcgcgcc agagccgcca
     2041 cctgggccac cgggccgagc aggtggacga gcgccgtcag gtagtacggc gccatgtcga
     2101 acagcggccc cgcgccggcg cggtagaaga agccggggtt ggggtgccag ctctccggcc
     2161 cgtggcccag aaagaaggcc gtggccgcca ccggctcgcc aatggccccc tcgtcgatca
     2221 gccggcggca ggtttgcagg ccaccgccga ggaaggtgtc gggcgcgcag ccgacgcgca
     2281 gcccctggcc cgcggctgcg tccagcaccg cccggccgtc ggcccgctcc acggccagcg
     2341 gcttctcgct gtaggcgtgc ttgcccgcgg ccaggatggc caggctgacc gaggcgtgga
     2401 cgctgggcac ggtcaggttg ataacgatgt cgatgtcggg gtcagccagc agcgcctcga
     2461 cggagccggc gcaggccagg tcgtgctcgg ccgccttggc ctgcgccacc tccggccgta
     2521 agtcggccac gccggtgacg cgcaagaagt cgaaggcgcg gcagccgttg atgtaggcgc
     2581 tgctgatgtt gccgcagcca atgatgccaa cgcgggtaat ctcaggcatg gccgtgaccc
     2641 aaccccttgc cgaccagata gcgctggctg gcctcgacag cggtgagcat gtccgtggcg
     2701 cagcggtcta tctcgacgat cagccactca gccgctgtgg ccgcggggat gatggtcgtg
     2761 tagtcgagcg cgcccgtgcc cacggcagtc atgggcgatt gcgtgccgtc ggccgggccg
     2821 tccttaacgt gcagcaactc cacccgcggc cccatccggc gcagcgcctc cagcggcgcg
     2881 acgccgccga ccctggccca gtaagcgtcc agctcgaaga aaatgtgtgg ctcgagccgc
     2941 tccagccaga cctcatacgg ccgctggccc gtgccggcca gcggctcgaa ctcaaaccag
     3001 tggttgtgga cgcccagccg caggccatgc ccggcgcaga cagacgcggc ctcgttgagt
     3061 tggtcgatgg cgcggtaggt gctgtccaac gtggcatacg tctccggcgg cagccaggcg
     3121 cagaccagcc gctcgcagcc cagagcttgc aactgcgcca acgtcgtggc cgcggcgtcg
     3181 cccaatggca acggcgcgtg ggccgaacag atgaccaggc ccaattcgtc gaacagggct
     3241 ttggccgccg tgggcgagac gccgtcgggg aagccggccg tctcaacgcc ggcgtagccg
     3301 atggcggcca cggcgcggat ggttccctcg aagtcgcggg ccagcgcctc gcgcagggag
     3361 tagagttgca gggcaatcgg tttggtcata gggaacttac ctcattgtct ggagatggtg
     3421 gtcaggagcc agagccacca gccactggcc acgcaccact tctgttcttc agtcgcaatc
     3481 ttatcgcacg cccccgcgcc gaccaactgg ggcatttcat gctacaatgt tgccatgttt
     3541 cgcgccgccc gtctgaccct gattttcgcc gttctgttcg cgttctttct catcgccccg
     3601 gcattcctga accagccctt tggcccctat ccgctcttgc gcgttggcaa cattctcgac
     3661 ctgctgacgc cgctggtact catcccggtc tactggctgc tcttccaggt agcgcccgac
     3721 cggcgcatca ccgaccgcga gacgctcgtc tttctggtgc tggccggcct atgggttatg
     3781 ggccactcct tgcacctggc cgccaacgcc atcggccacc tgaacgaggg tctggctggc
     3841 gacgcgcccg cgctgaccca cttcctcgac gaagacctca gccacaccat ctggctgctg
     3901 ggcattctcg gcctggcggc gctgatcctc tatcgccagt ggcgccaccc cttcgccggg
     3961 ccgcgccagg gaaatggcct ggtgctggcc gccgcggcca tccacggctt caactacttc
     4021 ctgagcatcg tcgaggcggg caccaatggg ctgggcatcg gctttgccgt gaccgtcgtt
     4081 gtcttcgggc tgttgcaacg gcgagagcta ggccagcggc ccatcacgcg cttcttcgtc
     4141 tttgcctatg ccatctcact ggtcttcttc ctcgcctggg gcctctactg gggcgggctg
     4201 ccggagttta gtcaggtcgg actgctaaac tgagccgcac gcggacgccc atgcttgacc
     4261 aatcgcccct gtccgacgac accctgttgg cctgcgcccg cgagcgcttt ggccggccag
     4321 ccgtggccat caccttcctt cccctcggct acgacgccaa tgcctgggcc taccggctgg
     4381 aggcagacaa agcatatttc ctgaaagtgc gccgcggccc ggcggccttg cccggcctgc
     4441 tcgcgccccg cgccgtcgcc gacgcgggca tcgacggcgt tctgaccccg ctgcccacga
     4501 ccgacggcgc gctctgggca gcgctgggcg acttcaccgt gacgctgcaa ccctttgtcg
     4561 ccggccgcga cgcctgggac agcggcctca gcgacgccca atggctggcc ttcggcaacc
     4621 tgttgcgtcg tgtccatgcg ctgcccgtcc cgccggcgct ggccgcgcac ctcgtcctcg
     4681 agcggtttgt gcccgacgac atggccttca tgcccgccct ggccgcccac gcccacgggg
     4741 ccgcccacgt ccacggcccg cggccggacg acccgctggc cgctggtctg gccgcctgct
     4801 ggcggcaaca cgagacaacg atcgccacgc tccagcacca ggccgccgtc ctgggcgagc
     4861 aattgcgccg ggccgacccg ccgcgcgtcc tttgccacgc cgacgcctac ctgggcaacg
     4921 tcctggtggg cgatgacggc cggctgtggc tcatcgactg ggacggcgcg gccctggccc
     4981 cccgcgagcg cgacctgatg ttcgtcttcg gcggcatcgc cgcggggcag gccgggccgc
     5041 gcgagacggc gctcttcgtc cgcggctatg gccccgtagc ccccgacgcg gcggcactga
     5101 cctactaccg ctgcgcctgg gtgttgcaag acctggcctc gtttggccat acggtgctgg
     5161 acgccgccgc cggcgaagcc acgcgccgcg aggcgctgcg gctgtttaag agcaatttcg
     5221 cgccaggcga gcttgccgcc ctggccctgt cgcgcgtcgc aactaccata gagggagacc
     5281 atgtttcttg atctgaacca acccaagcac gcccacgtcg atgcccgtct gcgggccgag
     5341 tcgatcctgt ggctgggcac cgtccggccc gacggccgcc cgcatctcgt ccccgtctgg
     5401 ttcctgtggg atggcgagag catcagcatc tacagccagc cggatgccca gaagctgcgc
     5461 aatctggccg ccaacccggc cgtcaccgtg gccctggagg cgctggacga gggcagcgac
     5521 gtcgccatca tcgaaggcga ggcccgggtc ggggccgcgg ccgacgcgcc ggcggtcgcc
     5581 gccgcctata ccgacaagta cgccgcggcc atcgccgacc tgggctatac cccggagggg
     5641 atgctggggg cctacagtcg cctcattcgg gtgacgccgc ggcgcttgct ggcctggtcc
     5701 ggctagcgcg gcttttcagt ctgcatgtaa tacagcctgt caggctgtcg ctggctacag
     5761 cctaacaggc tgtgctacgg gctgttgtaa tacagcctgt caggctg
//
LOCUS       DAOSMQ010000171         5777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14033_length_5777_cov_3.570255, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000171 DAOSMQ010000000
VERSION     DAOSMQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14033_length_5777_cov_3.570255"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="PK829_07085"
     CDS             complement(<1..1760)
                     /locus_tag="PK829_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU41016.1"
                     /translation="MRRLGFFCLSVLVLALGAARAQAAPPIPISDGPIRVTAYVLVRA
                     TGDPAQWANGARVEHLFHDWYRLYAPAGQTVETTLSAAADAPGVEAVSYDYIIHVDAP
                     RQLPAALVPGDSLYVRQWHMPQVQAEAAWDAIDGEGAIIAILDTGVRTGGPDGLCQPL
                     VAEYNAMTNSTAPQAARDNYGHGTHVAGTAAGCAGNGVGVTGLAYGARVMAVKVLADN
                     GNGSFSDVAEGIVWAADHGAGVINMSLGSACSTTWPGCSTAIVNDAIDYAAAADVVIV
                     ASAGNDNRTFAGQPGNHPEAISVSAMDFARNRAPYSNRGVAVDLGAPGGNTGADLSGD
                     GFADGVLQETFNANGVWGYYYYQGTSMAAPHVTGAVGLLRSCVPQATRADVRAALEST
                     AEDLGTAGFDTTYGNGFLQIRAAMDALATQYGQDAANGCAFVGDPPCLTVTTAVIGAG
                     QVAISPPSNCMAGDTPGYLFDTDLTVTATPDPGNGFAGWSDDAAGTANPLSLTRYGDM
                     AITATFGACRTVTAAPVGPGAVGVQPSPNCDTGYHDGTVLTLTATPDPGAVFTGWGGD
                     AGGMTNPLALTVSGDLAVTAG"
     gene            complement(1852..2847)
                     /locus_tag="PK829_07090"
     CDS             complement(1852..2847)
                     /locus_tag="PK829_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41017.1"
                     /translation="MNLRFNRTHLVALLIVLLFSLGVATVAAVPGDYTAVYVSAGGAG
                     TTEDGLAYQPGDIIGSLGPGQPWFTYFEAAAHGLGGKNINAFDLHLTETTVTDVVSEP
                     VYMSFSQSRVRLPGVSGPNGLATPNDIVKFSPLVPTAAGDSFELFFDGSDVGLSGRDE
                     QIDSLSVWLPEDFAPAAVGVPQDCTAGLLFITTAANYRVRDANGVSMPGKGGDILAFC
                     ATNLGPNTAGFWFRAFSATEAGFSPLRAIRNLTVHNFAYNSGTGDWELRFSFSSRTDF
                     HMGAFTGYANNVYLMDTTAGDPILELDLNTDYPTLNGSADGLEIVPYTSVCANDC"
     gene            complement(3109..4206)
                     /locus_tag="PK829_07095"
     CDS             complement(3109..4206)
                     /locus_tag="PK829_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41018.1"
                     /translation="MNTYFFRRRSSAVAVVALLLLFAAACVTDPGAVLLSMQKSGTTA
                     DGLAYGPEDIIAVTDDGSGPEWFTFFDGSEHGLTTKHALNAFSFNEFLLSSLSTGGPV
                     TQEDVLDIPEFYMTFRPNSIHVPGIPGRVTGQDIIKYSETDSSDPGTFEVIFDGSDVG
                     LTTRTEKIDGLGYWAPETYPDPLYAEACPAGIFFITTQGLYRVPGEVGQIVGSGFDVL
                     LFCATNVGPNTAGFWYRVFNNRDNNYFYPGRASTSLDVIGFELAPTVTAALAGNGPGV
                     NVHQPDLDAGIDFLFVPRTAFGINGATDRIFEPSQLYEVDSLTNYPDIDFYGPYADFN
                     AGEDYFNDDTFPPVNGLVQSMSIWDFPFTTP"
     gene            4503..>5777
                     /locus_tag="PK829_07100"
     CDS             4503..>5777
                     /locus_tag="PK829_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU41019.1"
                     /translation="MTNPPEFRPAAAEAWFAARGWSPFPFQYEVWRAYLAGRSGLVHA
                     ATGTGKTQAAWWGPVLEYLEEEKEGGGAGERRSEGETKRRTKAVVPLRVVWLTPMRAL
                     AADTERALREVVDDLGLPWSVERRTGDTGSAARARQMTRPPTALITTPESLSLLLSQP
                     NNELLFAGLRGVVVDEWHELLGNKRGVQTELCLARLRRLCPGLRTWGLSATLGNLDEA
                     LAALLGPEQAAAGVLVQGHMPKDIVIDTLIPERVERFPWAGHMGLRMLPQVAAAVEEG
                     RSALVFTNTRNQAETWYQALLAERPEWAGEIALHHGSLSADARRWVEDGLRAGTLRCV
                     VATSSLDLGVDFSPVDRVLQVGSPKGVARLLQRAGRSGHQPGATSRVTCVPTHAFELI
                     ESAAARDALRDGRLEGREPVERPLDVLVQHLVT"
BASE COUNT          991 a         1876 c         1968 g          942 t
ORIGIN      
        1 ccggccgtca ccgccaggtc gccgctgacc gtcagcgcca acgggttcgt catgccgccg
       61 gcgtcgccgc cccagccggt gaagaccgcc ccggggtcgg gcgtcgccgt cagcgtcagg
      121 actgtgccgt cgtggtagcc cgtgtcgcag ttgggcgacg gctgcacgcc gaccgcgccc
      181 ggcccaacag gcgcggccgt caccgtgcgg caagcgccga acgtcgccgt gatggccatg
      241 tcgccatagc gcgtcagcga cagggggttg gccgtgccgg cagcgtcgtc gctccagcca
      301 gcgaacccgt tgccggggtc tggcgtggcc gtcaccgtca ggtcggtgtc gaacagatag
      361 ccgggcgtgt cgccggccat gcagttagat ggcgggctga tggccacctg gcccgccccg
      421 atcacggccg tcgttacggt caggcagggc ggatcaccga cgaaggcaca gccattggcg
      481 gcgtcctggc catactgtgt cgccagcgcg tccatggcgg cgcggatctg caggaagccg
      541 ttgccatagg tggtgtcgaa gccggctgtg cccaggtctt cggccgtcga ttccagcgcg
      601 gcgcgcacgt cggcgcgcgt cgcctggggc acgcaactgc gcagcaggcc caccgccccg
      661 gtcacgtgcg gcgcggccat cgacgtgccc tggtagtagt aatagcccca gacgccattg
      721 gcgttaaatg tctcctgcag caccccatcg gcaaagccgt cgccgctcag gtcggcgccc
      781 gtgttgccgc ccggcgcgcc caggtcaacg gccacgcccc gattggagta gggcgcgcgg
      841 ttgcgggcga agtccatggc cgacacggag atggcttcgg ggtggttgcc cggttggccg
      901 gcaaaggtgc ggttgtcgtt gcccgcggag gcgacgatga ccacatcagc cgcggcggcg
      961 tagtcgatgg cgtcgttgac aattgccgtg ctacaccccg gccaggtggt tgagcaggcg
     1021 ctgccgagcg acatgttgat gacccccgcg ccgtggtcgg ccgcccagac aatgccctcg
     1081 gcgacatcgc tgaagctgcc gttgccgttg tcggccagca ccttgacggc catgaccctg
     1141 gccccatagg ccagaccggt cacgcccacg ccgttgccgg cgcacccggc ggccgtgccg
     1201 gcgacgtgcg tgccgtggcc atagttgtcg cgggcggcct ggggcgcggt ggagttggtc
     1261 atggcgttgt attcggccac caacggctgg cacaggccat ccggcccgcc cgtgcgcaca
     1321 ccggtgtcca ggatggcgat gatcgcgccc tcgccgtcga tggcgtccca ggccgcctcg
     1381 gcctgcacct gcggcatgtg ccactggcgc acgtagagcg aatcgccggg aacgagggcg
     1441 gccggcaact gccgcggcgc gtccacgtgg atgatgtagt cgtaggacac cgcctccaca
     1501 ccgggcgcgt ccgcggctgc gctcagcgtt gtttccaccg tttgacccgc tggggcatag
     1561 agacgatacc aatcgtggaa caggtgctca acgcgcgccc cgttggccca ctgcgccggg
     1621 tcgccggtcg ccctgaccaa aacataggcc gtcacgcgaa ttggcccgtc agagatgggt
     1681 attggcgggg cggcctgggc gcgcgccgcg cccagagcca gcactaaaac ggacaggcag
     1741 aagaaaccca atcgccgcat ggcaacctcc ttgcagagaa agaatggcgc gacggcaacc
     1801 tctcgttgcc gtcgcgtcaa ttgccgatag gggactaagc gcgccgtggc atcagcagtc
     1861 gttggcgcag actgacgtgt agggaacaat ctccaggccg tcggcgctac cgttgagcgt
     1921 cggatagtcg gtgttcaggt caagttccag aattgggtcg ccggctgtgg tgtccatcag
     1981 atagacattg ttggcatagc cggtgaacgc gcccatgtgg aagtcggtgc gcgaggaaaa
     2041 gctgaaccgc agctcccaat cgcccgtgcc gctgttatag gcgaagttgt ggacagtcag
     2101 attgcgaatg gctcgcagag ggctgaagcc ggcttcggtc gcgctgaagg cccggaacca
     2161 gaagccggcc gtgttggggc cgaggttcgt ggcgcagaag gccaggatgt cgccgccctt
     2221 gccgggcatc gacacgccgt tggcgtcgcg gacacggtag ttggctgccg tggtgatgaa
     2281 cagcaggccg gcggtgcaat cctggggcac gcctacagcg gcgggcgcga agtcctccgg
     2341 cagccagacc gacaggctgt cgatctgttc atcgcggcca gacagaccca catcggagcc
     2401 gtcgaagaac agctcgaacg aatcgccagc cgcagtgggc acaagtggcg agaacttgac
     2461 gatatcgtta ggcgtcgcca aaccgttggg gccagagacg ccgggcagcc gcacgcggct
     2521 ctggctgaag gacatgtaga ccggctcgct caccacgtcg gtgacggtag tttcggtcag
     2581 gtgaaggtcg aaagcgttga tgttcttgcc gcccaggcca tgggcggcag cttcaaaata
     2641 ggtgaaccag ggctggcccg gtcccaggct gccgatgatg tcgcccggct gataggctag
     2701 gccgtcttcg gttgtgcccg cgccgccggc gctgacgtag acggcggtat agtcgccagg
     2761 cacggcggcc accgtagcga cgccaagtga gaacagtaag acgatcaaca gagcgacaag
     2821 gtgggtgcga ttgaagcgca gattcatggg gattctccta aatatacagt gacataagca
     2881 actcagtccg acatccccga aacggggatg gcatccccga aacagggatg ttgcgctcct
     2941 atccttctac cctgccactg aacctgtccc ttacccacat attaagtcta aacgcatgcg
     3001 catgcaagca aatggacccg cccaatacaa ccatgccggc cgcgggcgag acgcgcctcg
     3061 ctcgcggccg gcactctcta ttgccggttc agcactgggg ccgataggct acggcgtggt
     3121 gaacgggaag tcccagatgg acatgctctg caccaggccg ttcacgggcg ggaaagtatc
     3181 atcgttgaaa tagtcctcgc ccgcgttgaa gtcggcatac ggcccataga aatcaatatc
     3241 cgggtagttg gtgaggctgt ccacctcgta gagctgcgac ggctcgaaga ttctgtccgt
     3301 ggcgccgtta atgccaaagg ccgtccgtgg cacgaagagg aagtcaatgc cggcgtccag
     3361 gtcaggctgg tggacattga cccccgggcc gttgccagcc aacgccgccg tgacggtcgg
     3421 ggccaactca aagccaatga cgtccaggct ggtgctggcc cggccggggt agaagtagtt
     3481 gttgtcgcgg ttgttgaaga cgcgatacca gaagccggcc gtgttggggc cgacgttggt
     3541 ggcgcagaac agcagcacgt cgaagccgct gccgacgatc tggcccacct cgccgggcac
     3601 acggtaaagg ccctgggtgg tgatgaagaa gatgcccgcc gggcaggcct cagcatagag
     3661 cgggtcgggg taagtctccg gcgcccagta gcccagaccg tcgattttct ccgtccgcgt
     3721 cgtcaggccc acgtcggagc cgtcgaagat cacctcaaac gtgcccggat cgctgctgtc
     3781 cgtctcagag tacttgatga tgtcctggcc cgtgacacgg ccggggatgc cgggcacgtg
     3841 gatggagttc ggccggaagg tcatgtagaa ctcagggatg tccagcacgt cctcctgggt
     3901 gaccggtccg ccggtggaca ggctgctgag caggaattcg ttgaaactga aggcattgag
     3961 cgcgtgcttg gtggtcaggc cgtgctcgct accatcgaag aaggtgaacc actccggccc
     4021 gctgccgtcg tctgtcaccg cgatgatgtc ttccgggcca taggccaggc catcggccgt
     4081 tgtgcccgac ttctgcattg atagcagcac cgcgcctggg tcggtcacac aggcggcggc
     4141 gaagagcagc agcaatgcca caacagcgac cgctgagcta cgccggcgaa agaagtaggt
     4201 attcatctgg tctcctaatc tgtgatactg agcaatgaac tacgtcgcca ccctacacat
     4261 accagggctg tagatgcgcg tatccacagc gcggtagcat gcgttctaac catcggcacg
     4321 acagacttct ctccgtcagg agctgtccct cgttattccc agcccctgac ttcctaagag
     4381 agagtcattg taaaatagcc gcatacggag cgcaagacct tttctctgcg tatttcagcc
     4441 ggttttgggg ccgtcgaatg acgtgttacc cctgcgagcc agggtggact acaattcccg
     4501 gcgtgacgaa tccgcccgaa ttccgcccgg ccgcggccga ggcctggttt gccgcccgcg
     4561 gctggtcgcc gtttcccttc caatatgagg tctggcgggc ctatctggcc ggccgcagcg
     4621 gccttgtcca cgccgccaca ggcacgggca agacgcaggc ggcctggtgg gggccggtgc
     4681 tggagtactt ggaggaggag aaagagggcg ggggagcggg ggagcggcgg agcgaggggg
     4741 agacgaagag gcgaacgaag gctgttgtgc ccttgcgggt cgtgtggctg acgccgatgc
     4801 gggcgctggc ggccgacacc gagcgagcgc tgcgcgaggt cgtcgatgac ctggggctgc
     4861 cgtggtcggt cgaacgccgc accggcgaca ccggcagcgc cgcccgcgcc cgccagatga
     4921 cccgcccgcc aacggccctc atcaccaccc cggaaagcct ctccctgctc ctctcccagc
     4981 ccaacaacga actcctcttc gccggcctgc gcggcgtcgt cgtcgatgag tggcacgaac
     5041 tgttgggcaa caagcgcggc gtgcagacgg agctttgcct ggcccggctg cgccgcctct
     5101 gccctggcct gcggacgtgg ggcctgtcgg ccacgctggg caacctcgac gaggccctgg
     5161 ccgcgctgct tggcccggag caggccgccg ccggcgtcct cgtccagggc cacatgccca
     5221 aggacatcgt catcgacacg ctcatccccg aacgggtgga gcgcttcccc tgggccgggc
     5281 acatggggct gaggatgctg ccgcaggtgg ccgcggccgt cgaggaggga cgctcggcgc
     5341 tggtcttcac caacacgcgc aatcaggccg agacgtggta tcaggccctg ctggccgagc
     5401 ggccggagtg ggccggagag atcgccctgc accacggctc gctgtcggcc gacgcccgcc
     5461 gctgggtcga agacggcctg cgcgccggga cactgcgctg cgtcgtggcc acgtcgtcgc
     5521 tcgacctggg tgtggacttc tcgcccgtcg atcgcgtctt gcaggtgggc agccccaagg
     5581 gcgtggcccg cctgctgcaa cgcgctggcc gcagcggcca ccagcccggc gcgaccagcc
     5641 gcgtcacctg cgtgcccacc cacgcctttg aactgatcga gtccgccgcc gcccgcgacg
     5701 ccctgcgcga cggccgcctg gaggggcgcg aaccggtgga gcggccgctc gacgtgctgg
     5761 tgcagcacct ggtcacc
//
LOCUS       DAOSMQ010000172         5765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14081_length_5765_cov_3.671629, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000172 DAOSMQ010000000
VERSION     DAOSMQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14081_length_5765_cov_3.671629"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="dnaN"
                     /locus_tag="PK829_07105"
     CDS             complement(<1..645)
                     /gene="dnaN"
                     /locus_tag="PK829_07105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU41020.1"
                     /translation="MKISCKQENLAKGLSIVGRAISPRSTLPVLGNVLLAVDNGRLRL
                     SATNLEIVITCWIGATVQEAGATTVPARTFSELINALPADHVDLTLNKTTETLHVNCG
                     KVQANIKGINADEFPVIPEPDQENRVRMEPAVLKRVIAQVAFAAATDDSRPSLTGVLT
                     TFEGNRIMMAATDGFRLSVRSAQLAGYVEKPFNVIIPARALNEVARILSDDLEAV"
     gene            complement(736..1179)
                     /locus_tag="PK829_07110"
     CDS             complement(736..1179)
                     /locus_tag="PK829_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU41021.1"
                     /translation="MLQSVEYPANVGSIFRLADGAGVTQLVLTGITPTPPNATIDKVG
                     RFKSLRLPWRYEADPVAAVEGLKSSGYRVVAVELTDDALPYYDYAYAPKTCLVVGHED
                     HGVTKATLAVCDAAVFIPMYGKGLSLNVHVALAVVLYHIRHAGGG"
     gene            complement(1275..>5765)
                     /locus_tag="PK829_07115"
     CDS             complement(1275..>5765)
                     /locus_tag="PK829_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU41022.1"
                     /translation="AAVIDTGVDFATTDLQGTPARVNGGPYDGWPYAYDTLSGAYYAL
                     DSVSIGPDNYWEQAANTFYAHTFPVEGATCADGVCSGELTLDFGATFDEVRLDFTWPD
                     SSKSGNYLYSVYPDYAHLLIGFYRGLGYANSLGTPAALIVADETTAGVYDTVYVDADF
                     DQDLVEEKPMSKGDELSGADLYGADWSDTLDGLWDISVSMLSWIADGENPPPGVGVLY
                     PNIAVPEQGRLIAFVGDADAHGTNVASMIAAQSIVTDPYRISHINPRFAGGAEAGGVG
                     GPVMAGMAPDAKIAAFMNGFNLPYDAWTLAVLGFDGQPQTGDEAQVVNNSWGDSSVVQ
                     DGWDDTARVAQYLNKTFAPSTLFLAATGNGGPGYGTTTTPNGGTILKVGASTSYGTST
                     FFEDVSPDLFTYGAIQPCSNRGPSGLGDVDPDLVCVGAWGQGATPLNRLPNGAGAYDL
                     FGGTSMSSPVCAGIATLVYQSFQEAHGRWPTWQEATDILLNSAIDLGYNALAQGAGNT
                     DALRATAIAAGDAAYVTPSQWQVGDYRGEDLTPGFPAIVHPGESKSLALTFHNPTDVP
                     VSSAVEDVTLLRTHAISFTVTLGASTDDEGLGLVPTYLRDITDLVEQYDPDLMSAHVM
                     FPQSRFDDLEDYYSDNAVTTLFYDWKDLNGDGNLWTDDDANGFVDPEELDRDHADGWE
                     FNRYTYAYAEANYQLADLGREALSRRHDGVFLGLRRDLGADDLDVTVQIIFYQKADWD
                     WLSLSAAAVNVPANGEAALTATMAVPAGARLGLYEGAIEYDGHVLPVIAHVAANSTTF
                     DFGAASLEETLGDTPYDNGHLLGSTDWAWRPETGDWKQFFYDVPEGTTDAGTGMIVTT
                     EWQYPRALEVPPLPEDYLYETFDTGIPASWFVFDNLGTCPWLTNADFGMDNLTGGEEL
                     AATASSNACVGPADTELYTPPIDLSSADQAWLAFRTHFIGNYDWEGNLLDHAIVEITT
                     DEGLTWTELLNWQTDYRGPWVIDLSAYTGSEVLLRFHYVTTGWSYWWQLDNVGVFAED
                     PTPSYNVPQRDLTDVNTALFGASEDDFSAGDPGFFGPTGLGLIGASRNTWLTGGKWMF
                     YTETGGPKEIVGGLATDGLGLLSLHNVLNAGRVVGEPVVGHAYQLSVDPAPLTAEATT
                     VTGANPPTMATQIDATVEATAGIPNGLGMLAFGPSQLDERPNQAITQEIASSPCSART
                     IIPVTVVDGGLLEVTTTTSEANLDIDLYLLLDDGDGVFACNQEYLVAQSITATADEHV
                     AVKLPDDGKYFILIHGYSVPRNPSLYDLTIRAIQGHGLTLNNVPNGAVAANTPLPFQI
                     NATVAYKPNSTWEGLLFLGPQEAPLALSIPVTITVPELAPGDLSARLNAAPDALATGE
                     QTTASLRIWNNSTDGEVVRATISVPPGLTVDPASLSATIGQVHFSVLERAITWTGLLD
                     GSSGATITFQATAATAARQVEMSAHVQGVLRDDELRLTTPLWLNVDRPPILSHMPIVA
                     RN"
BASE COUNT          956 a         1766 c         1995 g         1048 t
ORIGIN      
        1 gactgcctcc aggtcgtccg acagaatgcg cgccacttcg ttgagcgctc gcgccgggat
       61 gatgacgttg aacggcttct cgacgtagcc ggcaagctgg gccgaacgca ccgacaagcg
      121 gaagccatcg gtggcggcca tcatgatgcg attgccctcg aaggtcgtca ggacgccggt
      181 cagcgatggc cgcgagtcgt cggtggcggc ggcaaaagcc acctgggcga tgacccgctt
      241 gagcacagcc ggctccatgc gcacgcggtt ctcctggtcg ggttcgggaa tgaccgggaa
      301 ctcgtcggcg ttgatgccct tgatgttggc ctgcaccttg ccgcagttga cgtgaagcgt
      361 ctcagtggtc ttgttaagcg tcaggtcaac gtggtcggcc ggtagcgcgt tgatcagttc
      421 gctgaacgtc cgcgccggca cagtcgtcgc ccccgcttcc tgcaccgtcg ccccgatcca
      481 gcaggtgatg acgatctcca ggttggtggc cgagagcctg agccggccgt tatctaccgc
      541 cagcagcacg ttgcctaaaa cgggcaacgt tgagcgcggg ctgatggcgc gaccgacgat
      601 gctgagtcct ttggccaggt tttcttgctt gcaggagatc ttcatgacca cacgctccag
      661 ttaattgttg tactatcccg catgatattg gtgagtatac cccgacagac gagagtacgc
      721 aagaactcgc gccaatcagc cgccgccggc gtggcggatg tggtagagca cgacggccag
      781 ggccacatgg acgttgagcg atagcccctt gccatacatg ggaatgaaca cggctgcgtc
      841 gcagacggcc agggtggctt tggtcacgcc gtggtcttcg tggccgacaa cgaggcacgt
      901 tttgggcgcg taagcgtagt cgtagtaggg cagggcgtcg tcggtcagct cgacggcgac
      961 gacacggtag ccgctcgatt tcagcccctc caccgcggcg acgggatcgg cctcgtagcg
     1021 ccagggcagg cgcaggctct tgaagcgacc gaccttgtcg atggtcgcgt tgggcggtgt
     1081 cggcgtgatg ccggtcagca ctaactgggt gacgcccgcg ccgtcggcca gccggaagat
     1141 ggagccgacg ttggccggat attcgacgct ctgtagcagg acggcgaggt cgtgggccgg
     1201 cgggtgcgcg cggcggtagt cgcggacgaa gcgcttcagg tcagcgccga tgaggagtga
     1261 gtctagcgtt taatctagtt gcgggccact atcggcatat ggctcaggat tggcggccgg
     1321 tcgacgttga gccagagcgg cgttgtcagc cgcagctcat cgtcacgcag gacgccctgc
     1381 acgtgcgcgc tcatctcgac ctggcgggcg gccgtggccg ccgtcgcctg gaaggtgatc
     1441 gtcgcgccag agctgccgtc gagcaggcct gtccaggtga tggcgcgttc taggacgctg
     1501 aagtgtacct gcccgattgt ggcgctgagc gaggccgggt caaccgtcag cccgggcggc
     1561 acgctgatgg tggcgcgaac cacctcgccg tcggtactgt tgttccagat tcgcaggctg
     1621 gccgtggtct gctcgccggt cgccaacgcg tcgggcgcgg cattcaggcg agcgctcagg
     1681 tcgccggggg ccagctccgg cacggtgatg gtgaccggaa tgctcagcgc caacggcgct
     1741 tcctgcggcc ccaggaacag cagcccttcc caggtcgaat tgggtttgta ggccaccgtg
     1801 gcgttgatct ggaacggcag aggcgtgttg gcggcgacag cgccattcgg cacgttgttc
     1861 agcgtcaggc cgtgaccctg gatggcccgg atggtcaggt catacaggga tggatttctg
     1921 ggcacgctgt agccatggat caggatgaag tatttaccat cgtcgggcag cttcacggcg
     1981 acatgctcgt cggccgtggc cgtgatggac tgggcgacga ggtattcctg gttgcatgcg
     2041 aacacgccgt cgccgtcgtc gagcagcagg tacaggtcga tgtcgagatt tgcctcagac
     2101 gttgtcgtcg tgacctccag caggccgcca tcaacaacgg tgaccgggat gatcgttctg
     2161 gccgagcagg gcgagctggc tatctcctgc gtaatcgcct gattaggccg ctcgtccagt
     2221 tgcgacgggc caaacgccag catgcccagc ccgttgggga tgccggcggt ggcctcgacc
     2281 gtggcgtcga tctgggtcgc catggtgggc gggttggcgc cggtgactgt cgtggcctcg
     2341 gccgtcagcg gcgcggggtc aaccgacaac tgataggcgt ggcccacgac cggctcgccg
     2401 acaacacgcc cggcattgag cacattgtgc agggacagga gacccaggcc atcggtggct
     2461 aggccgccga caatttcctt cgggccaccc gtttcagtgt aaaacatcca cttgccgccg
     2521 gtcagccagg tgttgcggct cgcgccgatc aggcccaacc ccgtcggccc gaagaaaccg
     2581 gggtcgccgg cggagaagtc gtcctcgctc gcgccgaaga gagccgtgtt cacgtcggtc
     2641 aggtcgcgct gggggacgtt atagctgggc gtcgggtctt cggcgaagac gccgacgttg
     2701 tccagttgcc accagtagct ccagcccgtc gtgacgtagt ggaaccggag caacacctca
     2761 ctgccagtat aggccgatag atcaatcacc cacgggccgc gatagtcggt ctgccagttg
     2821 agcagttccg tccaggtaag accttcgtct gtggtgatct caacaatggc atggtctaac
     2881 aggttgcctt cccagtcgta gttgccgatg aagtgtgtgc ggaaggccag ccaggcctgg
     2941 tcggcgctgc tcaggtcgat gggcggtgtg tatagttccg tgtcggccgg gccaacgcag
     3001 gcgttggagc tggctgtggc agccagttcc tctccgccgg tgaggttgtc catgccgaag
     3061 tcggcattgg tcagccacgg acaagtgccc aggttgtcga aaacgaacca tgaggccgga
     3121 atgcccgtat cgaacgtctc gtagaggtaa tcctccggta ggggcgggac ttcgagcgcc
     3181 cgcgggtact gccactcggt tgtgacgatc atgcccgtgc cggcgtcggt cgtgccctcg
     3241 ggcacgtcat agaaaaactg cttccagtcg cccgtctccg gccgccaggc ccagtcggtc
     3301 gagccgagca ggtggccatt gtcgtagggt gtgtcgccca gcgtctcctc cagcgaggcg
     3361 gcgccgaagt cgaacgtcgt cgagttcgcg gcgacgtggg cgatcaccgg cagcacgtgg
     3421 ccgtcatact cgatcgcgcc ctcatagagg cccaggcggg cgccggcggg cacggccatc
     3481 gtcgccgtca gcgcggcttc gccgttggcc ggaacgttga ccgcggcggc cgagaggctc
     3541 agccaatccc agtccgcctt ctggtagaag atgatctgaa cggtcacatc caggtcgtcg
     3601 gcgcccaagt cgcgccgcag gccgaggaag acgccgtcgt gccggcgcga cagggcctcg
     3661 cgccccaggt cggccagttg gtagttggct tcggcataag cgtaggtgta gcggttgaac
     3721 tcccacccgt cggcgtggtc gcggtcaagc tcctccggat cgacgaagcc gttggcgtcg
     3781 tcgtcggtcc acaggttgcc gtcgccgttc aggtctttcc agtcgtagaa cagcgttgtg
     3841 accgcgttgt ccgagtagta atcctccaga tcatcgaagc gcgactgggg gaacatcacg
     3901 tgggcgctca tcaggtcggg gtcgtattgc tcgaccaggt cggtgatgtc gcgcaggtag
     3961 gtgggcacca ggcccaggcc ctcatcgtct gtgctggccc ccagcgtcac cgtgaagctg
     4021 atcgcgtggg tgcgcaggag ggtcacgtct tccacagcgc tgctgacggg cacgtcggtc
     4081 ggattgtgga aggtcagcgc cagcgactta ctctcgccgg ggtgaacgat ggccgggaag
     4141 ccgggcgtca ggtcttcgcc gcggtagtcg ccgacctgcc attggctggg cgtcacgtag
     4201 gcggcgtcgc cagcagcaat ggccgtggcg cgcagcgcgt cggtgttacc cgcgccctgg
     4261 gccagggcgt tgtagcccag gtcgatggcg ctgttgagca gaatgtccgt cgcctcctgc
     4321 caggtgggcc aacgaccgtg ggcttcctgg aaggactggt agaccagcgt ggcgatgccg
     4381 gcgcacaccg ggctggacat cgacgtgccg ccgaagaggt cataggctcc tgccccattg
     4441 ggcaggcgat tgagcggcgt cgcgccctgt ccccaggccc cgacacagac caggtcggga
     4501 tcgacatcgc ccaggcccga cgggccgcga ttcgagcacg gctgaatcgc gccgtaggtg
     4561 aacaggtcgg ggctgacgtc ctcgaagaag gtgctggtgc cgtaggaagt gctggcgccg
     4621 accttgagga tcgtgccgcc gttgggcgtg gtcgttgtac catagccggg accgccattg
     4681 cccgtggccg ccaggaagag cgtcgagggg gcaaaggtct tgttcagata ttgggccacg
     4741 cgggccgtgt catcccagcc gtcctggacg acgctgctgt cgccccagga gttgttaacg
     4801 acctgggcct catcgcccgt ttgcggctgg ccgtcgaagc cgagcacggc cagcgtccag
     4861 gcatcatacg gcagattgaa gccgttcatg aaggcggcga tcttggcatc gggggccatg
     4921 ccggccatca ccgggccgcc cacgccgccg gcttctgccc cgccggcgaa gcgcggattg
     4981 atgtgactga tccggtacgg atcggtgacg atactctgcg cggcgatcat cgaggccacg
     5041 ttggtgccgt gggcatcggc gtcgccgacg aaggcaatca ggcggccctg ctcgggcacg
     5101 gcgatgttgg ggtagagcac gcccacgccg ggcggtgggt tctcgccgtc ggctatccag
     5161 ctgagcatgc tcacggagat gtcccatagc ccgtccagag tgtcgctcca atccgcgcca
     5221 tagagatcgg cgccggacag ttcgtccccc ttggacatgg gcttttcctc gacgaggtcc
     5281 tggtcgaagt cggcatcgac atagacggtg tcatagacgc cggccgtggt ctcgtccgcg
     5341 acgatcagcg ccgcgggcgt gcccagcgag ttggcatagc ccagaccacg atagaagcca
     5401 atgagcagat gagcatagtc ggggtagacg ctatagaggt agttgccgct cttcgaacta
     5461 tccggccagg tgaaatcgag gcgcacctca tcaaaggtgg ccccgaagtc gagggtcaac
     5521 tcgcccgagc agacaccgtc cgcgcacgtc gccccctcca ccgggaacgt gtgggcatag
     5581 aacgtgttgg ccgcctgttc ccagtagttg tccgggccaa tgctgaccga atccagcgca
     5641 tagtacgcgc cggagagtgt gtcataggcg tagggccagc cgtcataggg gccgccgttc
     5701 acgcgcgccg gggtgccttg caggtcggtt gtggcgaagt caacgcccgt gtcgatcacg
     5761 gcggc
//
LOCUS       DAOSMQ010000173         5742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14155_length_5742_cov_4.187093, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000173 DAOSMQ010000000
VERSION     DAOSMQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14155_length_5742_cov_4.187093"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..751
                     /locus_tag="PK829_07120"
     CDS             98..751
                     /locus_tag="PK829_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOU41023.1"
                     /translation="MTGATPSRLHQGQPTQRAGQPLGEAGAAMILIHGRGATAESILE
                     LAGYLPHPAMTYLAPQAAGQTWYPYSFLAPLSQNEPYLSSALARLGDLLDEVTAAGIS
                     PERIVWTGFSQGACLAAEFVARRAQRYGGVLLFSGGLIGPPGTPRAYEGQLAGTPVFL
                     GCSDRDAHIPVARVEETAEVLTRLGAVVDRRIYPRMGHTINDDEIAAAAALVAAVRV"
     gene            complement(748..1131)
                     /locus_tag="PK829_07125"
     CDS             complement(748..1131)
                     /locus_tag="PK829_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41024.1"
                     /translation="MTVLEDTALLITASRDLAASLAALLLSIPPLRHVERVPGPVAFM
                     QRLSTDRQPALVVLDTDEVGPATSQVIETMRTVSPETRCLVLSNSVAESRELAADGIE
                     PVVIKGASPSRLVDAIENLLGDSGD"
     gene            complement(1285..1953)
                     /locus_tag="PK829_07130"
     CDS             complement(1285..1953)
                     /locus_tag="PK829_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU41025.1"
                     /translation="MDKAIRILICDDHAVVREGLRALIGTEPGMEIVGEAADGEQAIA
                     AVRSLRPDVTLLDMVMPRMDGLEAIQIIKQEMPDTRILVLTSFAEDDMVFPAIKAGAL
                     GYLLKDSSPDELIRAIRDVNRGEASLHPSIARRLILELSQPTTLPPTPEPLTERELEV
                     LRLVAKGYSNEEIASTLIVSERTARGHVSSILSKLHLANRTQAALYALREGLASLGDS
                     GDSI"
     gene            complement(1922..3712)
                     /locus_tag="PK829_07135"
     CDS             complement(1922..3712)
                     /locus_tag="PK829_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU41026.1"
                     /translation="MQPELVASGTLFARIFHASPVAMAIYARNDGRCVAANDAFARLF
                     GYDAGALVGQRVEDLGLLDDEARRQLGTTVLSAGRLADRPLRVRARDGSFHDVLASIQ
                     LASWGDETFLIALLQDLTDYHRARRALSEAEARFRLFFEAIPVPVVVYDGDTLAILDV
                     NATTTAQYGYSREELLTMSALALWPAEEHATCAACLRSGDVVPLAQAYHYRRDGGRIA
                     IEMTSQSVTLDGRRAYLATFADVSEQQANAAARRHSEEQLHILADVTTDVIWSYDPLR
                     QTITYSSGMSTLFGHAAGEWPSPKGWLDKVHPDDRDRVMRELAEALPSKEGRWTSQYR
                     FRRADDTYAHVLDRGFVLRDTGEGVHFIGAMVDITRQIELQEATARATAEERRRLARD
                     LHDAVTQSVYSLTLLSAAARRRVSQGETGASFDYVERLGQLARQALKEMRLLVYELRP
                     ATLETEPLVTALQTRLEAVEQRAGVATRLRVEAEPHLPPHVQAQLFRVAEEALNNTLK
                     HASATAVDVLIGGDARHLRLEIRDDGIGFLPDPATTPAGLGLVSMRERVSELGGTFAV
                     DSIPGRGTTVRVVLELGNGDNGQSDPHSDL"
     gene            complement(3745..5580)
                     /locus_tag="PK829_07140"
     CDS             complement(3745..5580)
                     /locus_tag="PK829_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU41027.1"
                     /translation="MLLPVFAPGTLFAHIFHSSPVAMTINTLPDGQYVDVNDVFCHLV
                     GYSRQELIGHRAIDLGILTAERREPVIQELVAQGALGKRSQVLRHRSGEEHHVLVSAQ
                     LEEHEGQLYTVAIIQDLTDYLRTQDALQTTEQRFGLFFDSIPLPVWVFDVETLRILDA
                     NPAACSAHHYTAEELRAMTVVDVLTAEEGERFRDYAAQIEQWPTYNGIWRHQRKEGAP
                     MDMATTGYTLSLNGRRVRLAVLRDITQQLAVERALIDGERRLQIITELSTDGIWDFDV
                     RGGMVHLNEAFRRIYGAPASASDVHTALDWWLSRIHPDDRAAVLRNLQETTASSGYYF
                     SSELRLLRADNVHYATVISRGHILRDDDGQVTRITGAMVDISRQMEIAEATAQATLEE
                     RRRLARDLHDSVTQSLYSITLLAEVARRHAQSGNASATLEQIERLGALSQQCLREMRL
                     LVYELRPAMVEESGLVGALRQRLEAVEQRSGVHVHLDASDDQRIPLPIQNELFRVAEE
                     ALNNALKHAHASQLTIGLQTTVDEVALSIADNGRGFDAHVARTAGGQGLSNMAERVSR
                     LGGRFHIDTAPGQATTIRVHLPLPSADQWRLRWPQASLSLNGNRV"
BASE COUNT          962 a         1765 c         2022 g          993 t
ORIGIN      
        1 ccggcgtggt gaatgccttg gcggcgctat cggccgagga gcagcttacg ctggcggcgc
       61 tctgtcgtcg cctggggctg gcgcaggggc agccggtatg accggggcaa caccatcgcg
      121 cctccaccag ggacagccca cgcagcgggc gggccagccg ttgggcgagg ccggcgcggc
      181 catgatcctg attcacggcc gtggggccac ggccgagagc atcctggaac tggccggcta
      241 tctgccacac ccggcgatga cctatctggc cccacaggcg gccgggcaaa cgtggtatcc
      301 ctacagcttc ctggccccgt tgtcgcagaa cgagccatat ttgtcttcgg cgctggcgcg
      361 gctgggcgac ctactggatg aggtgactgc cgccggcatc tcaccggagc gcattgtctg
      421 gacgggcttc tcgcaggggg catgcctggc ggccgagttc gtcgctcggc gcgcgcaacg
      481 ctacggcggc gtgttactgt tcagcggcgg cctgatcggc ccgccaggga cgccgcgggc
      541 ctatgaaggt cagttggcgg gcacgcccgt ctttctgggg tgcagcgacc gggacgccca
      601 tatccccgtg gcccgggtag aggagacggc tgaagtgctg acgcggttgg gagcggtggt
      661 agaccggcgc atttatccgc gcatggggca cacgatcaac gacgacgaaa tcgccgccgc
      721 ggcggcgctg gtcgcggccg tgcgggtcta gtcgccgctg tcgcccagca ggttctcgat
      781 ggcgtccacc aggcggctgg gggaagcgcc cttgatgacc accggctcga tgccatcggc
      841 ggccaactcg cgcgattcgg ccacactgtt gctcaggaca aggcagcgcg tttccgggga
      901 gacggtgcgc atggtttcga tcacctgcga tgtggccggg cctacttcgt cggtatcgag
      961 gacgaccagg gcgggttgcc ggtcggtgct caggcgttgc atgaaggcca ccgggccagg
     1021 cacacgttca acgtggcgca atggtggaat cgacagaagc agcgccgcca ggctcgccgc
     1081 cagatcgcgc gatgcggtaa ttagcagggc tgtatcttcc aatacggtca tatgcactcg
     1141 gttcctctga cagaagtcta acaggttagc ctggcgcgat catcggcaaa gtgacgagac
     1201 aggcgaccgc aaatgacccc cttccggccc ggccggaagg gggtcaaatc aatccccttg
     1261 agtaaaacgt atgagtgcgg gcggctagat ggagtcgccg gaatccccca aagaggccag
     1321 cccctcgcgc aaggcataga gcgcggcctg ggtgcggttg gccaggtgca gcttgctgag
     1381 gatgctgctg acgtggccgc gggccgtgcg ctcgctgacg atcagcgtgc tggcgatctc
     1441 ctcgttcgag tagcccttgg ccaccaggcg caggacttcc agctcgcgct cggtcagcgg
     1501 ctccggcgtg ggcggcagcg tcgtcggctg gcttagctcc aggatgagcc ggcgggcgat
     1561 gctggggtgc agcgatgctt cgccccggtt gacgtcgcgg atggcgcgga tgagttcgtc
     1621 gggcgaggag tccttcagca gatagcccag cgcgccggcc ttgatggccg gaaagaccat
     1681 gtcgtcttcg gcaaacgagg ttagcacgag gatgcgcgtg tcgggcatct cttgcttgat
     1741 gatctgaatg gcctccagcc cgtccatgcg cggcatgacc atgtccagca gggtcacgtc
     1801 ggggcgcaga gagcggacgg cggcaatggc ctgttcaccg tcggcggcct cgccaacgat
     1861 ctccatgccc ggctcggtgc cgatcagggc gcgcaggccc tcgcgcacga cggcatgatc
     1921 atcacaaatc agaatgcgga tcgctttgtc cattgtctcc attccctaat tccaaaacta
     1981 cacggacggt cgtaccccgg ccggggatcg aatcgacggc gaaggtgccg cccaattcgc
     2041 tgacgcgctc gcgcatactc accaggccca ggccggccgg cgtggtggcg gggtcgggca
     2101 agaagccgat gccatcgtcg cggatttcaa ggcgcagatg gcgcgcgtcg ccgccgatga
     2161 ggacatcgac ggccgtggcc gaggcatgct tgagggtatt gttgagcgct tcctcggcca
     2221 cgcggaagag ctgggcctgg acgtgcggcg gcaggtgggg ttcggcctcc acgcgcagcc
     2281 gggtggccac gccggcgcgc tgctccaccg cttccaggcg ggtttgcagc gccgtcacca
     2341 gcggctcggt ctccagggta gccggtcgca gttcgtagac cagcaggcgc atttccttca
     2401 acgcctggcg ggccaactgg cccagacgct caacgtagtc gaatgaggct cccgtttcgc
     2461 cctggctgac gcggcggcgg gcggcggccg acagcagcgt caggctatag accgactgcg
     2521 taacagcgtc gtggaggtcg cgggccaggc ggcggcgctc ttcggccgtg gcccgcgcgg
     2581 tggcctcttg caactcgatc tgccgggtga tgtcaaccat cgcgccgatg aaatgcacgc
     2641 cttcgccggt atcgcgcaag acgaagccgc ggtcgagcac atgggcatac gtatcgtcgg
     2701 cgcggcgaaa gcggtactga ctggtccagc ggccctcttt gctgggcagc gcctcggcta
     2761 actcgcgcat gacccgatcg cggtcgtcgg gatgcacttt gtctagccag ccctttgggg
     2821 aaggccactc gccggcggcg tggccgaaga gggtgctcat gccgctgcta taggtgattg
     2881 tctggcgcag ggggtcgtag ctccaaatga catcggtggt cacgtcggcc agaatgtgga
     2941 gctgctcctc gctgtggcgg cgggcggcgg cgttggcctg ctgctcgctg acgtcggcga
     3001 aggtggccag gtaggcgcgg cggccgtcca gagtaacgct ctggctggtc atctcgatgg
     3061 cgatgcggcc gccgtcgcgc cggtagtggt aggcctgggc caggggcacg acgtcgccgg
     3121 agcgcaggca ggccgcgcag gtggcgtgtt cctctgccgg ccacagggcc agcgccgaca
     3181 tggttagcag ttcctcgcgg ctatagccat actgggccgt ggtcgtggca ttgacatcga
     3241 gaatggccag cgtatcaccg tcatagacga cgacgggcac gggaatcgcc tcgaagaaaa
     3301 ggcgaaagcg cgcctcggct tcgctcagcg cccggcgagc gcggtggtag tcggtcaggt
     3361 cttgcagcag ggcgatcagg aaggtctcgt cgccccacga cgccagctgg atggaggcca
     3421 ggacatcgtg gaaggagccg tcgcgcgcgc gaacgcgcaa cggccggtcg gccaggcggc
     3481 cggcgctgag gacagttgtg cccagttggc ggcgagcctc gtcgtctagc aggcccaggt
     3541 cttcgacacg ctggccgacg agcgcgccgg cgtcatagcc gaaaaggcgg gcgaaggcat
     3601 cgttggcggc gacgcagcgg ccgtcattgc gcgcataaat cgccatggcc acggggctgg
     3661 cgtggaagat gcgcgcaaac aatgtgcccg acgcgacaag ctctggctgc atggcaattg
     3721 ccgacgaaca gtaagctgtg gcgattatac gcgatttccg ttcaggctca agctggcctg
     3781 gggccagcgc agccgccact gatcggccga gggcaggggc agatgcacgc ggatggtcgt
     3841 ggcctggccg ggcgcggtat cgatatggaa gcggccgccc agccgggaga cgcgctcggc
     3901 catgttgctc agcccctggc cgccggcggt gcgggcgacg tgggcgtcga agccgcggcc
     3961 gttgtcagcg atggacaaag ccacttcatc gaccgttgtt tgcaagccga tagtcagctg
     4021 cgaggcgtgg gcgtgcttca gagcattgtt gagcgcttcc tcggccaccc ggaacagttc
     4081 gttctggatg ggcaggggga tgcgctggtc gtcgctggcg tcgaggtgga cgtgcacgcc
     4141 ggaacgttgc tccaccgcct ctagccgctg gcgcagggcg cccaccagcc ccgactcttc
     4201 taccatcgcc ggtcgcaatt catagacgag caggcgcatc tcgcgcaggc actgttgcga
     4261 caacgcgccc aaccgctcga tctgctccag cgtggccgag gcgttgccgg actgggcatg
     4321 tcgccgggcg acttcggcca gcagggtaat gctgtagagc gactgggtta ccgagtcgtg
     4381 caggtcgcgg gccaaccggc ggcgttcctc cagcgtggcc tgggcggtgg cttcggcgat
     4441 ctccatctga cggctgatat ccaccatggc cccggtgatg cgcgtcacct ggccgtcgtc
     4501 gtcgcgcaga atgtgccccc gggagatgac cgtggcgtaa tggacgttgt cggcgcgcag
     4561 aagacgcagc tcagacgaga aataatagcc ggagctggcg gtggtctctt gcaggttgcg
     4621 caaaacggcc gcgcggtcgt cgggatggat gcgcgacagc caccaatcga gggcggtgtg
     4681 gacgtcgctg gcgctggccg gcgcgccgta gatgcgccgg aaggcttcgt tgaggtgaac
     4741 catgccgccc cgcacgtcga agtcccagat gccgtcggtg ctcaactcgg tgatgatctg
     4801 caaacgccgt tcgccgtcta tcagggcgcg ctcgacggcc agttgctgag tgatgtcgcg
     4861 cagaaccgcc aggcgcacgc gacggccgtt gaggctgagc gtgtagccgg tggtggccat
     4921 gtccatcggc gcgccctctt tcctctggtg tcgccagatg ccattatagg tgggccactg
     4981 ctcgatctgc gcggcatagt cgcggaagcg ctcgccctcc tctgctgtca ggacgtccac
     5041 caccgtcatg gcgcgcagct cctcggcggt gtagtgatgg gcactgcacg cggccgggtt
     5101 ggcgtccagg atgcgcagcg tctccacgtc gaagacccac accggtaaag ggatactgtc
     5161 gaaaaacagg ccgaagcgct gctcagtggt ctgtaaggcg tcctgggtgc gcagatagtc
     5221 cgtcaggtct tgaatgatgg ccaccgtata gagctgccct tcgtgctcct ccagttgggc
     5281 cgagacgagt acgtgatgct cttctccgga gcgatggcgc agtacttgcg accgtttgcc
     5341 cagcgctcct tgggctacca actcctggat cactggctcg cggcgctcgg ccgtcaggat
     5401 gcccagatcg atggcgcggt ggccgatgag ttcctggcgt gagtaaccaa ccagatgaca
     5461 gaagacgtcg ttcacatcaa cgtattgccc atcgggcaaa gtgttgatcg tcatggccac
     5521 cgggctggaa tggaagatgt gggcaaaaag cgtgccgggt gcgaaaactg gaagaagcat
     5581 tgcgtgcgcg ccctataaga caaaaagaca cgcacattat accttgccgt ctggatttgg
     5641 ccatgagtta aaagacttat tatcagcacg tgcaaaggtc atggcttgct gggggccaag
     5701 cgaagtaatc ctaatccgga gccgtgggca cgggagccgt gg
//
LOCUS       DAOSMQ010000174         5734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14183_length_5734_cov_5.071139, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000174 DAOSMQ010000000
VERSION     DAOSMQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14183_length_5734_cov_5.071139"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..792
                     /locus_tag="PK829_07145"
     CDS             22..792
                     /locus_tag="PK829_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41028.1"
                     /translation="MTANDNGSAAGRPVIVIRDMAKVYRMGEYEVRALNGVSVDIRAG
                     EFVAIMGPSGSGKSTMMNMLGALDKPTSGQYLLDGIDVSDMSDDQLADIRNRKIGFVF
                     QNFNLLPRVPAIQQVELPLIYAAKRERGERARRALEMVGLGERVHHRPSELSGGQQQR
                     VAIARALVNEPAIILADEPTGNLDSKSGTEVMAIFQRLNREQGITILFVTHDPWIARH
                     TRRIITLADGVVVRDETVPTPLVAGQSKRPSDEALVHE"
     gene            871..2112
                     /locus_tag="PK829_07150"
     CDS             871..2112
                     /locus_tag="PK829_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU41029.1"
                     /translation="MNLALNTRLALGSLLANKLRAVLTMLGIIIGVAAVITLISVGEG
                     VQDVVVSEFEGLGNNLLFVVPGSPDANEFTGDLVRAELTNDDYLALANPSAVPDLVRV
                     APTYGRPAIVTRGGEEARTTVTGTTPDYTDVRDFYPIEGDFFTEQDVTTAARVAVLGQ
                     SVYEQLFPDGTPPVGETIRINNVNFRVIGLMEEKGGSGFNDQDDVVLIPLNTAQRRLF
                     PARRADGEYRVDQILAQVASEERQDAAIVEMGLALRDSRGIAYRDEEDFTILSQSDVI
                     GALSQITNVLTVFLGLIAGISLLVGGIGIMNIMLVSVTERTREIGLRKAVGAKRRDIL
                     WQFLTEAIILASLGGLIGLALGAGGAWLISRFSDLLDARLAWNSVALAILFSAAVGLF
                     FGIYPATRAARLNPIDALRYE"
     gene            2116..3363
                     /locus_tag="PK829_07155"
     CDS             2116..3363
                     /locus_tag="PK829_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU41030.1"
                     /translation="MLSENIRIALRGLSANKLRAALTMLGILIGVAAVITLLSLGDGV
                     TRYVADQFSGLGTNLIFVIPVQEQPGPPGSDPFNESSLTVRDATDIADTNLVPGAAAA
                     APVMFRQVELQFGGNLHTVLARAATPDYTAINTLTIARGRDIDQTDYDGRSRVVILGQ
                     DTANALFPDDVDPLDQEVRLNGINFRVIGLLTTKGAAGIGGSQDDIAIIPLTTAQERL
                     FDARSARTGQLLVDAIIVQAADEAAIDGVIIDIGELLRNNHQIAFRDDDDFRMLTQGD
                     FIQAFGQVTSVLTLFLGAIAGISLLVGGIGIMNIMLVSVTERTREIGLRKAVGAKRRD
                     ILGQFLTEAVVLALLGGIAGMLLGTLGAVAIDLAVPQLDTSVGLDSIALAVGFSAAVG
                     LFFGIYPAARAARLNPIEALRFE"
     gene            3375..3911
                     /locus_tag="PK829_07160"
     CDS             3375..3911
                     /locus_tag="PK829_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41031.1"
                     /translation="MATQNGGFQIRASLWLLGLALGLITMVGCAPTSDTVAPAATPAD
                     TVVLPAALRAFPQQLPVEGERARLTSIAEGQLALRDGCLRLASTDDATDSGYLIIWPG
                     DYEVKEEDGVLAIYDGQGHFLAKVGDDVVMGGGEDNQTTPLTDTVFGLNEPTPAECPG
                     PYWIMGDFVPPPGAVSAP"
     gene            4143..4751
                     /locus_tag="PK829_07165"
     CDS             4143..4751
                     /locus_tag="PK829_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41032.1"
                     /translation="MARKVVLWMTLLALLMALAACGGSTTTDNGNESQASNANDIVAT
                     DQPPGDAETIGDTTGGPGNTTSQGEAVTESGSTPESGAASDASGMAAPAFLNDWQTAG
                     ATVVTGGEVPASLFEGASGQTYLVNDVELQVYEFNDAAAAEAAAATISPDGGMINDSA
                     VRWAGPPHFFRQDNLIIVYIGDDAATLDLLGGTFGTPFAGPP"
     gene            complement(4772..>5734)
                     /locus_tag="PK829_07170"
     CDS             complement(4772..>5734)
                     /locus_tag="PK829_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU41033.1"
                     /translation="AKTSATGLATIPLGAGASDLSALGVTAAQGTTELARGTIIVGSQ
                     VANNFYDPRLRPGQEPPAPPDLYDQSLTIELIKWTEDGTEIRKKVRARVAGVLDEARD
                     EPDWSIYMPLSDVDGFNTWSTGQRIDRNKNGYNTVIVKVEDVDVVLDVTDQITALGFQ
                     AYTPQSFVQGISGFYLVLQVVFGAMGAIALLVAAIGIANTMTMAILERTREIGLMKAV
                     GASNRDVLAIFLGEAAGIGFIGGLGGVVISWGLGQVLNVIVLGYLAGQAAQTGAPPPS
                     GAIATPLWLPASALLFATLMGLASGLYPALSAATLAPVAALKYE"
BASE COUNT          955 a         2027 c         1816 g          936 t
ORIGIN      
        1 cgcagcgacg aaaagacgcc catgacggca aacgacaacg gcagcgccgc cggccggccg
       61 gtcatcgtca tccgcgacat ggccaaggtc taccgcatgg gtgagtacga ggtgcgcgcc
      121 ctgaatggcg tgtcggtgga tattcgcgcc ggcgaatttg tggccatcat ggggccgtcc
      181 ggctccggca aatcgacgat gatgaacatg cttggcgcgc tggacaagcc gaccagcggc
      241 caatacctgc tcgacggcat cgacgtcagc gacatgagcg acgaccagct ggccgacatc
      301 cgcaaccgca agatcggctt cgtcttccag aacttcaacc tcttgccgcg cgtgccggcc
      361 atccagcaag tcgaactgcc gctcatctac gccgccaagc gcgagcgcgg cgagcgggcg
      421 cggcgggcgc tggagatggt cgggctgggc gagcgcgtcc accaccggcc gagcgaactc
      481 tccggcggcc agcaacagcg cgtggccatc gcccgcgccc tggtcaacga accggccatc
      541 atcctggccg acgagccgac gggcaacctc gacagcaagt cgggcacgga ggtcatggcc
      601 atcttccagc gcttgaaccg cgagcagggc atcaccatcc tgttcgtcac ccacgacccg
      661 tggatcgccc gccacacgcg gcgcatcatc accctggccg acggcgtggt cgtccgcgac
      721 gagaccgtgc ccacgccgct ggtggccggg cagagcaagc ggccgtcaga tgaggccctt
      781 gtccatgaat gagatcgccg ctatgcccgg acgcgctctt cctgtaggtg tcctggtact
      841 ccggcgtcct tacgttcgtc tcttcagcct atgaacctgg ccctgaacac ccgcctcgcc
      901 ctcggcagcc tgctggccaa caagctgcgc gccgtcctga cgatgctggg catcatcatc
      961 ggcgtcgccg cggtcatcac cctcatttcc gtcggcgagg gcgtccagga tgtcgtcgtg
     1021 tccgagttcg aggggctggg caacaatctg ctcttcgtcg tgcccggctc gcccgacgcc
     1081 aacgagttca ccggcgacct ggtgcgcgcc gagctgacca acgacgacta tctggccctg
     1141 gccaatccga gcgccgtacc cgacctggtg cgcgtcgctc cgacctacgg ccgcccggcc
     1201 atcgtcaccc gcggcggcga agaggcgcgc acgaccgtca ccggcaccac gcccgactac
     1261 accgacgtgc gcgacttcta tcccatcgag ggcgacttct tcaccgagca ggacgtgacc
     1321 acggccgcgc gcgtggccgt gttgggccag agcgtctacg agcagctctt ccccgacggc
     1381 acgccgccgg tgggcgagac gattcgcatc aacaacgtca acttccgcgt catcggcctg
     1441 atggaggaga aaggcggctc cggcttcaac gaccaggatg acgtcgtcct catcccgctc
     1501 aacaccgccc aacgccgcct cttccccgcc cgccgggccg acggcgaata ccgcgttgac
     1561 cagattttgg cccaggtggc cagcgaggag cggcaggacg cggccatcgt cgagatgggc
     1621 ctggccctgc gcgactcgcg cggcatcgcc taccgcgacg aggaggactt caccatcctc
     1681 agccagtcgg acgtcatcgg cgcgctcagc cagatcacca acgtcctgac cgttttcctg
     1741 ggcctcatcg ccggcatctc gctgctggtc ggcggcatcg gcattatgaa catcatgctc
     1801 gtgtccgtca ccgagcggac gcgcgagatc ggtctgcgca aagccgtggg ggccaagcgc
     1861 cgcgacatcc tgtggcaatt cctgaccgag gccatcatcc tggccagtct cggcgggctg
     1921 atcgggctgg cgctcggcgc gggcggggcc tggctcatca gccgcttctc cgacctgctc
     1981 gacgcccggc tggcgtggaa ctcggtcgcc ctggccatcc tattctcggc cgccgttggc
     2041 cttttcttcg gcatctaccc ggccacgcgc gcggcgcggt tgaaccccat cgacgccttg
     2101 cgctatgagt aagccatgct gagcgaaaac atccgcatcg ccctccgcgg cctctcggcc
     2161 aacaagctgc gcgccgcgct gaccatgctc ggcatcctca ttggcgtggc cgcggtcatc
     2221 accctgctat cgctgggcga cggcgtcacg cgctacgtgg ccgaccagtt ctccgggctg
     2281 ggcacgaacc tcatcttcgt catcccggtg caggagcagc ccggcccgcc cggcagcgat
     2341 cccttcaatg aatcctcgct gacggtgcgc gacgcgaccg acatcgccga caccaatctc
     2401 gtccccggcg cggccgccgc cgccccggtc atgttccggc aggtggagtt gcagttcggc
     2461 ggcaatctcc acaccgtcct ggcccgcgcg gccacgcccg actacacggc catcaacacg
     2521 ctgaccatcg cccgcggccg cgatatcgac cagacagact acgacggccg ctcgcgcgtc
     2581 gtcatcctgg gccaggacac cgccaacgcc ctcttccccg acgacgtcga tccgctcgac
     2641 caggaagtgc gcctcaacgg catcaacttc cgcgtcatcg gcctgctgac gaccaagggc
     2701 gcggccggca tcggtggcag ccaggacgac atcgccatca tccccctgac cacggcccag
     2761 gagcgccttt tcgacgcgcg cagcgcccgc acgggccagc tcctcgtgga cgccatcatc
     2821 gtccaggccg ccgacgaggc cgccattgac ggcgtcatca tcgacatcgg cgaactgctg
     2881 cgcaacaacc accagatcgc cttccgcgac gacgacgact tccgcatgct gacccagggc
     2941 gacttcatcc aggcctttgg ccaggtgacg agcgtgctga cgctcttcct gggggccatc
     3001 gccggcatct cgctgctggt cggcggcatc ggcatcatga atatcatgct cgtgtcggtg
     3061 acggagcgga cgcgcgagat cggcctgcgc aaggccgtgg gggccaagcg gcgcgacatc
     3121 ctgggccagt tcctgaccga ggcggtcgtg ctggctctgc tgggcggaat tgcgggtatg
     3181 ctgttgggca cgctgggggc cgtggccatc gacctggccg tgccgcaact ggacacgtcc
     3241 gtgggcctcg actccatcgc cctggccgtg ggcttctcgg ctgcggtcgg cctcttcttc
     3301 ggcatctacc cggccgcgcg cgccgcgcgg ctgaacccga ttgaagcgct gcgctttgag
     3361 taaaggtcaa gactatggcg actcaaaacg gtggttttca gattagagct agtttgtggc
     3421 tactgggcct ggccttggga ctaataacga tggttggctg cgcccccacc tcagacacgg
     3481 tggctcctgc cgcgacaccc gctgatactg tcgtcttgcc cgcggctttg cgcgcctttc
     3541 ctcaacagct accagtagaa ggggaacgag cgcgcttaac atcgattgcc gagggtcaac
     3601 tggccttgcg cgacggctgt ctacgactgg cgagtaccga tgacgcaacg gactcgggct
     3661 atttgattat ctggccgggc gattacgagg tgaaagagga agatggcgtg ttggccatct
     3721 acgacggcca gggtcatttc ctggccaagg tcggcgacga cgtagtgatg ggcggcggcg
     3781 aagacaatca gaccacacca ctgaccgaca cggtctttgg cttgaacgag ccaacgcccg
     3841 cagagtgtcc aggcccctac tggattatgg gcgatttcgt accgccaccc ggcgctgtct
     3901 cagcccctta acctggaggt cgggttaacc cgccattgtc ccctgatagg gcctacctct
     3961 gctccgggca ttgtggcccg gtggggagca tacctcacgg cccccctggg cgaatccggc
     4021 tgttcgccgc ggtcgccaca acgcgccctt ctacccacgc tgcctgatca atcggccctt
     4081 gtggctgcgc gcttctcctg ctagaaaacg atcatcaatc tatgcacgga gagaagacaa
     4141 cgatggcgcg aaaagtggtt ttgtggatga ccctgctggc gctgctgatg gccctggcgg
     4201 cctgtggcgg cagcaccacg acggataacg gcaacgagtc gcaggcgtcg aacgccaatg
     4261 acatcgtggc caccgaccag ccccccggcg acgccgagac catcggcgac acgaccggcg
     4321 gcccgggcaa tacgaccagc cagggcgaag cggtgacgga gagcggcagc acgccggaga
     4381 gcggcgcggc ctctgacgcc agtggcatgg ccgcgccggc gttcctcaac gactggcaga
     4441 ccgccggggc gaccgtcgtc accggcggcg aagtgccggc gtcgctgttc gagggcgcca
     4501 gcggccagac gtatctggtc aacgatgtcg agttgcaggt ttacgagttc aatgacgccg
     4561 ccgcggccga ggcggccgcg gccacgatct cacccgatgg cggcatgatc aacgattcgg
     4621 ctgtccgctg ggccggcccg ccccacttct tccgccagga caacctgatc atcgtctata
     4681 tcggcgacga cgcggccacg ctcgacctgc tgggcggcac gttcggcacg cccttcgccg
     4741 gcccgcccta gccggagtaa cccatacccg gctactcgta cttcagcgcg gcgaccggcg
     4801 ctaacgtcgc cgcgctgagg gccgggtaga ggcccgacgc cagccccatc aacgtcgcga
     4861 agagcagcgc cgacgccggc aaccacagtg gcgtggcgat ggccccgctg ggtggcggcg
     4921 cgcccgtctg ggccgcctgc ccggccagat agcccaggac gatgacgttg agcacctgcc
     4981 ccagccccca gctaatgacc acgccgccca gcccgccgat gaagccgatg ccggccgcct
     5041 cgcccaggaa gatggccagc acgtcgcggt tgctggcccc gacggccttc atcaggccga
     5101 tctcgcgcgt gcgctccagg atggccatgg tcatcgtgtt ggcaatgccg atggcggcca
     5161 ccagcagggc aatggcgccc atggccccga agacgacctg tagcaccagg tagaagccac
     5221 tgatgccctg tacaaacgac tgtggcgtgt aggcctggaa gcccagcgcg gtgatctggt
     5281 cggtcacgtc gaggacgaca tcaacatcct cgaccttgac gatgaccgtg ttgtagccgt
     5341 tcttgttgcg gtcgatgcgc tggccggtag accaggtgtt gaagccatcc acgtcgctca
     5401 gcggcatgta gatcgaccag tccggctcgt cgcgggcctc gtcgagcacg ccggccacgc
     5461 gggcgcgcac cttcttgcgg atctccgtgc cgtcctccgt ccacttgatc agttcgatgg
     5521 tcaacgactg atcatacagg tcgggcgggg cgggcggctc ctggccgggg cgcaggcgcg
     5581 ggtcgtagaa gttgttggcc acctgcgagc cgacgatgat cgtgccgcgg gccagctccg
     5641 ttgtgccctg cgccgcggtc acgcccagcg cagacagatc gctcgcgccc gcgccgagcg
     5701 ggatcgtggc gaggccggtg gcgctggtct tcgc
//
LOCUS       DAOSMQ010000175         5724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14217_length_5724_cov_4.953607, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000175 DAOSMQ010000000
VERSION     DAOSMQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14217_length_5724_cov_4.953607"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="PK829_07175"
     CDS             <1..1280
                     /locus_tag="PK829_07175"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HOU41034.1"
                     /translation="AAAMTVSGRYATVGEVVAAAGLALGEGDRLSPAAGAPADPATPI
                     AIDRAAAVTVLTLDSAQTYHTQQTTLAGFLAEIGLIVPPGARLLADGQPVDAPGAQPL
                     PGQIAIDPYKTIIIVDGADRRRLRTTALTVGEALSEAGVSVAALDGVTPPRETALASD
                     MTITIDRAAPYTIHADGQTVAVFSGATVAGDVLADNGIALGALDYTRPALDAPVAPGD
                     VIEVIRVTERIDTEDVAIDFQTVYEAAADLEIDTIVEASAGSPGVLQRRTRVRLENGV
                     EVSRAPDGEVVTVPPVNHVIRYGTNIVMRTIDTEKGPVEYWRKVTMRVTSYTAATSGK
                     APGDPGYGITASGVQAGTGVVAVDPTVVPFKSYVYVPGYGVAFAGDTGGGIKGRFIDL
                     GYDEGQLKSWRGTVDVYFLGPPPPAEDIRWILP"
     gene            1277..2098
                     /gene="rsmA"
                     /locus_tag="PK829_07180"
     CDS             1277..2098
                     /gene="rsmA"
                     /locus_tag="PK829_07180"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOU41035.1"
                     /translation="MTHPKHILERAGLEPKKSLGQNFLFDDGLLARIVAAAELSPDDE
                     ALEVGPGLGALTRQLAAAARRVVAVELDERLLPLLESELAGLGNVELVHGDVLQLDLA
                     GWFAAPYVVVANVPYYITGAILRHLLGSWPRPRRLVLTVQREVADRLTARPPEMSLLA
                     VSVQHYGRVRLVSGVRAGAFWPRPEVDSAVVRIDVDARHLAARSPEAEAAEAAFFRVV
                     RAGFSEKRKQLKNNLRHLGHDDAAIGAALARAGVDGRRRAETLTVAEWAALSELL"
     gene            2216..2428
                     /locus_tag="PK829_07185"
     CDS             2216..2428
                     /locus_tag="PK829_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOU41036.1"
                     /translation="MNRQPVTSTNLVAVGFDPGSNILEVEFHSTRVYQYRGVPQAVYE
                     NLKEAESKGRFFNANIRQSYPYVRVQ"
     gene            2611..3360
                     /locus_tag="PK829_07190"
     CDS             2611..3360
                     /locus_tag="PK829_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU41037.1"
                     /translation="MLTIGQLARRVGLRPSALRYYEAEGLLRPSDRTEAGYRLYAPES
                     ADTVRLIQRAQRLGFSLAEIRGLLDAWRSGDLDNAELLATAEARYLALERQVTELRVQ
                     QHELELFLQDLRGPDAEAAQTAFGRLVDRICANPSAEPPSRTVLDWLSAYAECPLSGA
                     AANAVLAPLRGRHVHIWREEESYHILVVGHDPAVARALGELARLEADCSAHPGNGPQL
                     ADSAEGHLLVARGPNAFILARLFLALEEETP"
     gene            3489..4001
                     /locus_tag="PK829_07195"
     CDS             3489..4001
                     /locus_tag="PK829_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOU41038.1"
                     /translation="MDEQRFQIAGMDCADCARSIEKGVARLDEVDSAELNFTTGTLCV
                     RGSAPAERVVARVRELGYDVEDGRGAGAGAQGSTASLELSPAPRPPRSPAPLLAYFWG
                     RGDTRAALIAALLVLPGLVFDELLPGLGVASPVFAGMAVLAMIIAGWPIARSAVRACA
                     STARSPSTCS"
     gene            3998..>5724
                     /locus_tag="PK829_07200"
     CDS             3998..>5724
                     /locus_tag="PK829_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU41039.1"
                     /translation="MTIAAVGAVVIGAYTEAGLVMVLFALGEALEGYTAGRARETIRG
                     LSALAPAEATRLDDGGETRVPVAALRVGDRLAIRPGERLPMDGRVLAGASAVNQAPIT
                     GESRPVDKAPGDEVFAGSVNGPGALEIEVTHLAEDTTISRMIRLVAEAQEGRAPAQRF
                     VDRFARVYTPAVIVIALLVAIVPPLLFGAPFWNPASADGVSAHVSAHGWLYRALALLV
                     VACPCALVISTPVTLISAISNAARHGILFKGGAALEELSRVRAMAFDKTGTITRGEPA
                     VVAVRAAACVEPAATCAPCDELVALAAAVERRSEHPLAGAVLAEAEARGVAGRYPAGE
                     GVAALAGRGITGRVDGQTVSIGSHAWFDDAIDHLPHCDDIRAADDAGYTTMLVSSDDA
                     YRGYIALADATRAGSRAALAELAAQGIRPLVMLTGDHAATAGRIAAEVGLTDVRAGLL
                     PADKVAAVEALRAEHGGVAMIGDGINDTPALAAASVGIAMGRAPQALETADIALLGDD
                     LRQLPFAVRLARAAMRTVRVNVAVSIAIKLVFFVLVLLGSGSMWLAVLADVGTSLLVT
                     ANGMRLLRWP"
BASE COUNT          824 a         2083 c         1958 g          859 t
ORIGIN      
        1 atgcggcggc catgacggtc agcggccggt acgccaccgt cggcgaggtc gtcgccgctg
       61 ccgggctggc cctgggcgag ggcgaccgcc tctcccccgc cgccggcgcg ccggccgacc
      121 cggccacgcc catcgccatc gaccgcgccg ccgccgtgac ggtgctgacc ctcgacagcg
      181 cccagaccta ccacacccag cagacgacgc tggccggctt cctggccgag atcggcctga
      241 tcgtgccgcc cggcgcgcgg ctgctggccg acgggcagcc ggtggacgcg cccggcgcgc
      301 agccgttgcc cggccagatc gccatcgacc cctacaagac aatcatcatc gttgacgggg
      361 ccgaccgccg ccgcctgcgc acgacggcgc tgaccgtcgg cgaggcgctg agcgaggccg
      421 gcgtctctgt ggccgcgctg gacggcgtga cgccgccgcg cgagacggcc ctggccagcg
      481 acatgaccat caccatcgat cgggccgcgc cctacaccat ccacgccgac ggccagacgg
      541 tggctgtgtt cagcggggcc accgtcgccg gcgacgtgct ggccgacaac ggcatcgccc
      601 tgggggcgct ggactacacg cggccggcgc tcgatgctcc ggtcgctcct ggcgacgtca
      661 tcgaggtcat ccgcgtcacc gagcgcatcg acaccgagga cgtggccatc gacttccaga
      721 cggtctatga ggcggcggcc gacctggaga tcgataccat cgtcgaggcg tcggccggca
      781 gccccggcgt cttgcagcgg cgcacgcgcg tgcggctgga gaacggcgtc gaggtctcgc
      841 gcgcgcccga cggcgaagtc gtcaccgtcc cgcccgtcaa ccacgtcatc cgctacggca
      901 ccaacatcgt catgcgcacc atcgacacag agaaggggcc ggtggagtac tggcgcaagg
      961 tgaccatgcg cgtgacttcc tacaccgcgg ccacctccgg caaagccccc ggcgaccccg
     1021 gctatggcat taccgccagc ggcgtgcagg ccggcacggg cgtggtggcc gtcgatccga
     1081 ccgtcgtgcc cttcaagtcc tacgtctacg tgcccggcta tggcgtcgcc ttcgccggcg
     1141 acaccggcgg cggcatcaag gggcgcttca tcgatctggg ctacgacgag gggcaactga
     1201 agtcgtggcg gggcacggtc gatgtctact tcctcggccc accgccgccg gccgaagata
     1261 ttcgctggat tctgccgtga cccaccccaa gcacatcctc gagcgggccg gccttgagcc
     1321 aaagaagagc ctgggccaga acttcctctt cgacgacggc ctgctggcgc gcatcgtcgc
     1381 cgcggccgag ctgtcgcccg acgacgaggc gctggaagtc ggccccggcc tgggcgcgct
     1441 gacgcggcaa ctggccgcgg cggcccggcg cgtggtggcc gtggagctgg acgaacggct
     1501 gctgcccctg ctggagagcg agttagccgg gctgggcaac gtggagcttg tccacggcga
     1561 cgtgctgcaa cttgacctag ccggctggtt cgccgcgccc tacgtcgtcg tcgccaacgt
     1621 gccctattac atcaccgggg ccatcctgcg ccacctgctg ggcagttggc cgcggccgcg
     1681 ccggctggtg ctgaccgtgc agcgcgaagt ggccgaccgg ctgacggccc ggccgccgga
     1741 gatgagcctg ctggcggtca gcgtgcagca ctatggccgg gtgcggctgg tgtcgggcgt
     1801 gcgggcgggg gcgttctggc cgcggccgga ggtcgattcg gccgtcgtgc gcatcgacgt
     1861 tgatgcgcgc catctggctg cgcgttcgcc cgaggcggag gcggccgagg cggctttctt
     1921 ccgcgtcgtc cgcgccggct tcagcgagaa gcgcaagcag ctcaagaaca acctgcgcca
     1981 cctgggccac gacgacgcgg ccatcggcgc ggcgttggcg cgggcgggcg tcgatggccg
     2041 gcggcgggca gaaacgctga ccgtcgccga atgggccgct ctgagcgaac tgctctagaa
     2101 catttgtatc gttttctaat gaaaaataga cctatcctga ggtataatac ttgtgcgggc
     2161 caatacaacg aatgatttag cccggcacaa accctttgag gagaggtcta gaagtatgaa
     2221 ccgacaaccg gtcacctcca ccaatctggt cgccgtcggc tttgaccccg gctccaacat
     2281 tctggaagtg gaattccaca gcacgcgcgt ctaccagtat cgcggtgtgc cccaggccgt
     2341 atatgagaac ctgaaggaag cggagtcaaa gggacggttc ttcaacgcca acatccggca
     2401 gagctacccc tacgtgcgcg tccaataatt cgccgcgcgg gcagccccgc ttcaccccca
     2461 ccccactgtg agcgaccatt gacctgacag gtaggcggcc acgaatggcc gcctacctgc
     2521 caggtcggtg cgtctggggt aactttggaa aagccctgct gaccagtagc tcactatttt
     2581 ccccatcaga aatccggcta tcatcggggc atgctgacca ttggccaact cgcccggcgc
     2641 gtcggtttgc ggccgtcggc cctgcgctat tacgaagccg agggcttgct gcgtcccagc
     2701 gaccgcaccg aggccggcta ccgcctctac gccccggaga gcgccgacac tgtccgcctc
     2761 atccagcggg cgcaacggct gggcttctcg ctggccgaga ttcgcgggct gctggacgcc
     2821 tggcgcagcg gtgacctgga caacgccgag ttgctggcca cggccgaggc ccgctacctg
     2881 gccctggagc ggcaggtgac cgagttgcgc gtccagcagc acgagttaga gctgttcctg
     2941 caagacctgc gcggcccgga tgccgaggcg gcccagaccg ccttcggccg cctcgtcgac
     3001 cgcatctgcg ccaacccctc ggccgagccg cccagccgca ccgtcctcga ctggctgtcg
     3061 gcctatgccg agtgtccgct gagcggcgcg gcagccaacg ccgtgctggc ccccttgcgc
     3121 ggccggcacg tccacatctg gcgcgaagag gagagctacc acatcctcgt cgtgggccac
     3181 gaccccgccg tggcccgcgc gctgggcgag ctggcccggt tggaggccga ctgcagcgcc
     3241 caccccggca acggcccgca actggccgac agcgccgagg gccacctcct cgtcgcccgc
     3301 ggccccaacg ccttcatcct cgcccgcctc tttctggccc tcgaagaaga aaccccttga
     3361 ctttcaaccc gacttgaagg cgtataatca attaggaatt aggaattcaa gcgccaggcc
     3421 cggctctccc ttcttctttc ttgattccta attcttaatt tctgattcct aattgattga
     3481 gtcttccgat ggatgaacaa cgctttcaaa tcgcgggcat ggattgcgcc gactgcgcgc
     3541 ggtcgattga gaagggtgtc gcgcggctgg atgaagtcga ttcggccgaa ctgaacttca
     3601 ccaccggcac gctgtgcgtc cgcgggtcgg ctccggccga gcgtgtcgtc gcgcgggtgc
     3661 gcgagctggg ctacgacgtg gaagacggcc ggggagcggg ggcaggggcg caggggagca
     3721 ccgcaagcct ggaactctcc cccgctcccc ggcccccccg ctccccggcc cccctgcttg
     3781 cttacttctg gggccgcggt gacacccgcg ccgcgctcat cgccgcgctg ctggtattgc
     3841 cggggctggt cttcgacgag ctattgccgg ggctgggcgt cgcgtcgccc gtcttcgccg
     3901 gcatggccgt gctggccatg atcatcgccg gctggcccat cgcccgcagc gccgtgcgcg
     3961 cctgcgcgtc aaccgcgaga tcaccatcaa cctgctcatg accatcgccg ccgtcggcgc
     4021 ggtggtcatt ggggcctaca ccgaggccgg gctggtcatg gtcctgttcg cgctgggcga
     4081 ggcgctggag ggctacaccg ccggccgcgc ccgcgagacc atccgcggcc tgtcggccct
     4141 ggccccggcc gaggcgacgc ggctggacga cggcggcgag acgcgcgtgc ccgtggccgc
     4201 gttgcgcgtc ggcgaccgcc tggccatccg ccccggcgag cggctgccga tggacggccg
     4261 cgtcctggcc ggcgcgtcgg ccgtcaatca ggcccccatc accggcgaga gccgcccggt
     4321 ggacaaggcc cccggcgacg aggtctttgc cggcagcgtc aacggccccg gcgcgctgga
     4381 gatcgaagtc acccacctgg ccgaggacac gaccatcagc cgcatgatcc gtctggtggc
     4441 cgaggcgcag gaggggcgcg ccccggcgca gcgctttgtc gatcgcttcg cccgcgtcta
     4501 cacgccggcg gtcatcgtca tcgccctgct ggtggccatc gtgccgccgc tgctgttcgg
     4561 cgcgcccttc tggaacccgg cgagcgctga cggcgtcagt gcccacgtca gtgcccacgg
     4621 ctggctctac cgcgccctgg cgctgctggt cgtcgcctgc ccctgcgccc tggtcatcag
     4681 cacaccggtg acgctcatca gcgccatcag caacgccgcc cgccacggca tcctcttcaa
     4741 gggcggcgcg gcgctggagg aactcagccg cgtgcgggcg atggccttcg acaagacggg
     4801 cacgatcacc cgcggcgagc cggccgtcgt ggccgtccgc gccgccgcgt gcgtcgagcc
     4861 ggccgcgacc tgcgccccgt gcgacgagtt ggtggccctg gccgcggccg tcgagcggcg
     4921 cagcgagcac ccgctggccg gggccgtgct ggccgaggcg gaggcgcgcg gcgtcgccgg
     4981 gcgctatccg gccggcgagg gcgtcgccgc gctggccggg cggggcatca ctggccgcgt
     5041 ggacggccag accgtcagca tcggcagcca cgcctggttc gacgacgcca tcgaccacct
     5101 gccccactgc gacgacatcc gcgccgccga cgacgccggc tacacgacca tgttggtcag
     5161 cagcgacgac gcctaccgcg gctacatcgc cctggccgac gcgacacggg ccggcagccg
     5221 cgccgcgctg gccgaactgg ccgcgcaggg catccggccg ctggtgatgc tgaccggcga
     5281 ccacgccgcc acggccgggc gcatcgccgc cgaggtcggc ctgaccgacg tgcgcgcggg
     5341 gctgctgccg gccgataagg tcgccgccgt ggaagcgctg cgggcggagc acggcggcgt
     5401 ggccatgatc ggcgacggca tcaacgacac gccggcgctg gccgcggcct cggttggtat
     5461 agcgatgggg cgcgcgccgc aagcgctgga gacagccgac atcgcgctgc tgggcgacga
     5521 cctgcgccag ttgccgtttg ccgtccggct ggcccgcgcg gccatgcgca ccgtgcgcgt
     5581 caacgtcgcc gtgagcatcg ccatcaagct ggtcttcttc gttctcgtgc tgctggggtc
     5641 gggcagcatg tggctggccg tcctggccga cgtgggcacg tcgctgctgg tgacggccaa
     5701 tgggatgcgg ctgctgcgct ggcc
//
LOCUS       DAOSMQ010000176         5718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14240_length_5718_cov_4.188239, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000176 DAOSMQ010000000
VERSION     DAOSMQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14240_length_5718_cov_4.188239"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1128
                     /locus_tag="PK829_07205"
     CDS             4..1128
                     /locus_tag="PK829_07205"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU41040.1"
                     /translation="MPLVDLKTAYARLRAEIDAAVARVLAGGWYILGPEVSAFETEFA
                     AYLGAGHAVGVASGTDAVLLALRALGVGPGDEVITVAHTAVATVAAIALAGATPCFVD
                     IDTATYTLDPGRLEAAITPRTRAIVPVHLYGAPADMQPILAIAQAHGLLVIEDCAQAH
                     GATTDGGRVGTLGDAAAFSFYPTKNLGALGDGGAVVTKRPEVAERLRLLRQYGWRERY
                     VSDIEGYNSRLDELQAAILRVRLRHLDAENAARRRLAAQYDAALADLPLALPAAWPEE
                     RAVYHLYVVAADERDALAAYLAAQGIGTGVHYPVPIHCQPAYAHFGYGPGSLPATEAA
                     AARVLSLPMYPDLPAAAVDQVAAAIRSYFKAASRPPGAAH"
     gene            1142..2032
                     /locus_tag="PK829_07210"
     CDS             1142..2032
                     /locus_tag="PK829_07210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU41041.1"
                     /translation="MPPIPFSMGIMAHNEAANIGRLLARVAASRLERGEVAEIIVVAS
                     GCTDDTEAIVLAAAVADPRIRLITQPTREGKASAMNLFLREARCDVLVLSSADLLPAE
                     EALERLVSPFDDPETGLTACRPEPVNDPATFMGFAAHLLWDLHHEINLHGFKAGEMIA
                     FRKVFQRIPPHTAVDEASVEPLIRGQGYGVRYIPEAIVYNKGPETVEDFLRQRRRIYA
                     GHLEMQAQLGYSVSTMSGTKIAGHLLRHLDWRPRPFVWTWRVVALEMWGRYLGRRDYK
                     ARRSHTVWDIAATTKELQTP"
     gene            2038..3117
                     /locus_tag="PK829_07215"
     CDS             2038..3117
                     /locus_tag="PK829_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU41042.1"
                     /translation="MTGVLARVPLFQAYRRFGWPKLLPLNITLSPSPRCNSRCLTCNI
                     WMKRENELNLEEWDRVLASLGRAPYWFTISGGEPFMFSGIVELAQLAYKHCRPGIINI
                     PTNSILSTIPDKVERIARSCPESQLIINLSLDGVGEKHDLIRGVPGNFIRFEERLRQL
                     LALRDTLPNLNIGIHSVVSVFSVGHLDELIAYAEQSGADQFITEIAEPRVELDTVGLP
                     ITPSPEQYRAAIDRLIAYVESKRYKGVARITEAFRVEYYKLVKRILEEEDQVIDCYAG
                     WASAQIYADGTVWPCCVRADNLGNLRDHDYDFKAIWFGDKIKEVRRSIAAKECYCPLA
                     NASYTNMLIDPPTLTRVGLKVISPD"
     gene            3156..3590
                     /locus_tag="PK829_07220"
     CDS             3156..3590
                     /locus_tag="PK829_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU41043.1"
                     /translation="MPSLPDLRRFLADCYNDEELTGLCFDHFPEVFRNFAADMTTTRK
                     GIALLDYCQRRDRLPELLALLERERPEPFRRAFGATAPAADPRLNLNTATAGQLRQLP
                     GIGPVLAAAIIAARPLASVDDLLRVPGIGPRRLAAVRAWCDV"
     gene            complement(3615..4181)
                     /locus_tag="PK829_07225"
     CDS             complement(3615..4181)
                     /locus_tag="PK829_07225"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOU41044.1"
                     /translation="MQATLAALSLLLLAGMVLFRILWLRRSGTRAMHFGRLDKTDFLL
                     PPLMLFYIYTILAAAFGWPLASTRRLFQSEAVAWAGAALCLSGVIVMLLSLASFGRSF
                     RVGIDVEHPDQLVTTGIFALSRNPIYVAFGLVLLGQFLVFPNWVPLAYLLTGTVVIHR
                     QVLREEAFLRQHYGEAYTAYCRRVRRYV"
     gene            4333..4416
                     /locus_tag="PK829_07230"
     tRNA            4333..4416
                     /locus_tag="PK829_07230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4367..4369,aa:Leu,seq:cag)
     gene            4541..>5718
                     /locus_tag="PK829_07235"
     CDS             4541..>5718
                     /locus_tag="PK829_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41045.1"
                     /translation="MAVLLIVIVIFLVLLVGLLLAFLFSRADHAVSATRMAMAAEEKA
                     YNPGVTLGHKIKVQADYDAQLKEARLVAAKRAAGLPRGANNRIGRAGQSTLAPASKGL
                     QNDPQTAVRIARHHGWDGARVGPATAVAAAPAPVAAAAGPRPATAAPVAAGPGIAPPQ
                     LIAITDSMSPEEIRKARVANAKAESAYNKALKAATAGGSVPVAAPVAGAVAAPAATAA
                     GVPAAAGIAPPKLLAITPDMAPEDVRKARVANAKAEAAYNKALKAAGVGAGTVAGVEA
                     VVNAEMPGPATAVAHPQAAAPALAGIEPPQLIEITPGMSPEDVRKARVANAKAESAYN
                     KALKAAGVDPAALRGGAAAVATATSAPAPAVAAPAPAASVPAAAAPVAAAPAAPAAAG
                     "
BASE COUNT          956 a         2021 c         1845 g          896 t
ORIGIN      
        1 gcaatccccc ttgttgacct gaaaacggcc tatgctcgcc tgcgcgccga gatcgacgcg
       61 gcggtggcgc gcgtgctggc cggcggctgg tacatcctgg ggccggaggt cagcgccttc
      121 gagactgagt ttgccgccta tctgggcgcg ggccatgccg tcggcgtcgc ttcgggcacg
      181 gatgccgtcc tgctggcgct gcgggcgctg ggcgtggggc cgggcgatga ggtcatcacc
      241 gtggcccaca cggccgtggc caccgtcgcc gctatcgccc tggccggggc tacgccgtgc
      301 ttcgtcgaca tcgacaccgc cacctacacg ctcgatcccg gccggctcga ggcagccatc
      361 accccgcgaa cgcgggccat tgtgcccgtt cacctctacg gcgcaccggc cgacatgcag
      421 cccattctgg ccatcgccca ggcccacggc ctgctggtca ttgaggactg tgcccaggcc
      481 cacggggcga ccaccgacgg cggccgcgtg ggcacgctgg gcgacgcggc cgctttcagc
      541 ttttatccga ccaagaacct cggcgcgctg ggcgacggcg gcgcggtcgt caccaagcgg
      601 ccggaggtgg ccgagcggtt gcgcctgctg cgccaatacg gctggcgcga gcgctacgtc
      661 agtgacatcg aaggctacaa cagccgcctg gacgagttac aggcggccat cctgcgcgtc
      721 cgcctgcgcc acctggacgc ggagaacgcc gcccggcggc ggctggcggc tcagtacgac
      781 gccgcgctgg ccgatctacc cctggcactg ccggccgcct ggccagagga gcgcgctgtc
      841 taccacctct acgtcgtcgc cgccgacgag cgcgatgctc tggctgccta cctggccgcg
      901 cagggcatcg gcacgggcgt ccactatccc gtgcccatcc attgccagcc cgcctacgcc
      961 catttcggtt atggcccagg cagcctgccc gccaccgagg ctgccgctgc gcgcgtcctc
     1021 tctctgccca tgtatcccga cctgcccgcg gcggccgtcg accaggtggc cgcggctatt
     1081 cgttcctact tcaaagccgc tagccgaccg ccgggcgctg ctcactgaca actgaccgac
     1141 tatgcctccc atcccctttt ccatgggcat catggcccac aacgaggcgg ccaacatcgg
     1201 ccggctgctg gcgcgtgtgg ccgcctcgcg gctggagcgg ggcgaggtgg ccgagatcat
     1261 cgtcgtcgcc agcggctgca ccgacgacac cgaagccatc gtcctggccg ccgcggtggc
     1321 cgacccgcgc atccgcctca tcacccagcc cacgcgcgag ggcaaggcgt cggccatgaa
     1381 cctcttcctg cgcgaggcgc gttgtgacgt gctggtgctc agcagcgccg acctgctgcc
     1441 ggccgaagag gcgttggagc ggctggtcag ccccttcgac gacccggaga ccggtctgac
     1501 cgcctgccgg ccggagccag tcaatgaccc ggcgacgttt atgggctttg ccgcccatct
     1561 gctgtgggat ctgcaccacg agattaacct ccacggcttc aaggcgggcg agatgatcgc
     1621 cttccgcaag gtcttccagc gcatcccgcc gcacacggcg gtagacgagg ccagcgtcga
     1681 gccgctcatt cgcggccagg gctatggggt aaggtatata ccggaggcca tcgtctacaa
     1741 caaaggccca gaaacggttg aggacttcct gcgccaacgg cggcgcatct atgccggcca
     1801 tctggagatg caggcccagc tcggctacag cgtcagcacg atgagcggga cgaagatcgc
     1861 cgggcatctc ttgcgccatc tggattggcg gccgcggccg ttcgtctgga catggcgcgt
     1921 cgtcgcgctg gagatgtggg ggcgctacct gggccgccgt gactataagg cccgccgcag
     1981 ccacactgtc tgggacattg ccgcgacgac caaggagctg caaacaccgt gaattcaatg
     2041 acgggcgtat tggcccgtgt acccctattc caggcctacc ggcgcttcgg ttggccaaaa
     2101 ttgctgccgc tgaacatcac cctgtcgccc tcgccgcgct gcaactcgcg ctgcctgacg
     2161 tgcaacatct ggatgaagcg cgagaacgag ctaaacctgg aggagtggga tcgcgtgctg
     2221 gcctcgctcg gccgcgcccc ctattggttc accatcagcg gcggcgaacc gttcatgttc
     2281 tccggcatcg tcgagctggc ccagctggct tacaagcact gccggccggg gatcatcaac
     2341 atcccgacca acagcatcct cagcaccatc cctgacaagg tcgaacgcat cgctcgctcc
     2401 tgcccggaga gccaactgat cattaacctt tcgctagatg gcgtgggcga aaagcacgat
     2461 ctcatccgcg gcgtgcccgg caacttcatc cgcttcgagg agcgtctgcg acaattgtta
     2521 gccctgcgcg acactctgcc caatctcaac attggcatcc actccgtcgt cagcgtcttc
     2581 agcgtcggcc acctggacga gttgatcgcc tatgccgagc agtcgggcgc ggatcagttc
     2641 atcaccgaga tcgccgagcc gcgcgtcgaa ctggacactg ttggcctgcc catcaccccc
     2701 agcccggagc agtaccgcgc cgccatcgac cggctgattg cctacgtcga gagcaagcgc
     2761 tacaagggcg tcgcccgcat caccgaggcg ttccgggtgg aatactacaa gctggtcaag
     2821 cgcattctgg aggaggaaga tcaggtcatc gactgctacg ccggctgggc cagcgcgcag
     2881 atctacgccg acggcaccgt ctggccgtgc tgtgtccgcg ccgacaacct gggcaacctg
     2941 cgcgatcacg actatgactt caaagccatc tggtttggcg acaagatcaa ggaagtgcgc
     3001 cgcagcatcg ccgccaagga atgctactgc cccctggcca acgcctccta caccaacatg
     3061 ctcatcgacc cgccgacgct gacgcgggtg gggttgaagg tgatctcgcc ggattagcgg
     3121 ccaggcccca gaggtctcac cacccactga ccgccatgcc ttccctcccc gacctccgcc
     3181 gctttctggc cgactgttac aacgacgaag agctaaccgg cctgtgcttc gaccactttc
     3241 cggaggtgtt ccgcaacttc gccgcggaca tgaccacgac gcgcaagggc atcgccctgc
     3301 tagactattg ccagcggcgc gaccggttgc cggaactgtt ggcattgctg gagcgggagc
     3361 ggccagagcc attccgccgc gcctttgggg ccactgcgcc ggcggccgac ccgcgcctga
     3421 acctcaacac ggcgacggcc ggacagctcc gccaattgcc cggcatcggg ccggtgttgg
     3481 ccgcggccat cattgccgcc cggccgctgg cctctgtcga tgacctgctg cgcgtgcccg
     3541 gcatcggccc ccggcggctg gcggcggtgc gggcctggtg cgacgtctag cggcggaacg
     3601 gccctgccgg cggttcagac gtagcggcgc acgcgccggc aataggcggt gtacgcctcg
     3661 ccatagtgct gccgcaaaaa cgcctcctcg cgcaagacct ggcggtggat gaccactgtg
     3721 ccggtcagca gataggccag cggcacccag ttgggaaaca ccaggaactg ccccagcagc
     3781 accaggccga aggcgacgta gatggggttg cggctgaggg cgaagatgcc cgtcgtcacc
     3841 aactggtcgg ggtgctctac gtcgataccc acgcggaagc tgcggccgaa cgacgccaga
     3901 ctcagcaaca tgacgatcac gccggacagg cacagggccg cgccggccca ggcgacggcc
     3961 tccgactgga agagccgccg cgtgctggcc aacggccacc caaaggcggc ggcgaggatg
     4021 gtgtagatat agaacagcat cagcggcggc agcagaaagt cggtcttgtc gagccggccg
     4081 aagtgcatgg ccctcgtgcc cgaccggcgt agccagagga tacggaagag aaccattccc
     4141 gccagcagaa gcaaactcag agcggccagg gtagcttgca taggacgatc tcatcgagag
     4201 ccaggatgcg tcagccagca gcggctgcgc ggccattatc gcccgcccgc catgtactgt
     4261 ccatctgggc ggccgaaaga cgaccgatgt gtttgacaat tcgcgttgcc ctgttacaat
     4321 acccttcacc tagccgaagt ggcgaaactg gcagacgcgc tacgttcagg gcgtagtgag
     4381 ggttccctca tgtgggttcg aatcccacct tcggcatcat gacgctctat tcgttttcaa
     4441 gcgacccgga attcctccgg gtcgtttctt tttgagaaat ccagcacaat cgctataatg
     4501 tggctcatct ggcgctagat agagttgagg gactctggat atggctgttc tgttgatcgt
     4561 catcgtcatc ttccttgtac tcctcgttgg gttgctgctg gcctttctct tttcccgcgc
     4621 tgaccacgcc gtgtcagcga cgcgcatggc gatggccgcc gaggagaagg cctataatcc
     4681 gggcgtgacg ctgggccaca agatcaaggt ccaggccgac tacgacgcgc aactgaaaga
     4741 ggcacgtctg gtggcggcca agcgcgccgc cgggctgccg cgcggcgcaa ataaccgcat
     4801 cggccgcgcc gggcaatcga cgctggcccc ggccagcaag ggtctgcaga acgacccgca
     4861 aacggctgtg cgcattgccc gccaccacgg ttgggacggc gcgcgcgttg gcccggcgac
     4921 agccgtcgcc gccgcgccgg ctccagtggc tgcggctgcc gggccacgac ccgctaccgc
     4981 cgcgccggta gcagcaggcc ccggcattgc cccgccgcaa ctcatcgcca tcaccgacag
     5041 catgtcgccg gaagagatcc gcaaggcccg cgtggccaac gccaaggccg agtcggccta
     5101 caacaaggcg ctgaaggccg ccacggccgg cggcagcgtg cctgtggccg ctccggtggc
     5161 tggcgctgtc gctgcgcccg cggccacagc cgccggcgtg cccgcggcgg ccggcatcgc
     5221 gccgcccaag ctcctcgcta tcacgccgga catggcccct gaggacgtgc gcaaggcccg
     5281 tgtggccaac gccaaggcgg aagcggccta taacaaggcc ctcaaagccg ctggcgtcgg
     5341 cgcgggcacg gtggccggcg tcgaagccgt ggtcaacgcg gagatgcctg gcccggccac
     5401 ggcagtggcc cacccgcagg ccgccgcgcc ggcgctggcc ggcatcgagc cgccgcagct
     5461 gatcgagatc acgcccggca tgtcgccgga ggatgtgcgc aaggcgcgcg tggccaacgc
     5521 caaggccgag tcggcttaca acaaagccct caaggccgcc ggggtagacc cggcggcgct
     5581 gcggggcggg gccgcggccg tggccacggc tacctccgcg cctgcgcctg ctgtggcggc
     5641 gcctgcgccg gcggcgtctg tgccggcagc ggccgcgccc gtagccgccg cgccggcggc
     5701 cccggccgcc gctggtat
//
LOCUS       DAOSMQ010000177         5700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14309_length_5700_cov_3.848361, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000177 DAOSMQ010000000
VERSION     DAOSMQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14309_length_5700_cov_3.848361"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PK829_07240"
     CDS             complement(<1..401)
                     /locus_tag="PK829_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU41046.1"
                     /translation="MSDESMNSLEDLLDQHSYDMPQVGDIRTGVIVATTPQGLIIDLG
                     LKRDGIVPQSDLAQLEPEEREALKVNDEVPVYVVQTEDSEALVVSIRLARLNQDWIEA
                     EALLASGQIFEGEVIGYNKGGVIVPYGRIRG"
     gene            complement(939..1805)
                     /locus_tag="PK829_07245"
     CDS             complement(939..1805)
                     /locus_tag="PK829_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOU41047.1"
                     /translation="MNLLTYDHHGHPTPGLLRGEAIVDLSPVAPDLLTLIDGGAAALA
                     RVRALAKTASDALPLAGARLLAPIPIPRRNVICLGKNYAEHARESYEARGEQVVTTEY
                     PVIFTKATTTISGPYDDIAIDDAVSAQMDYEVELAFIIGRPTRRANRAEALAAVFGYT
                     VLNDVTARDLQNLHKQFHIGKSLDGCCPIGPWIVTADAVADPHALRITCRVNGELRQD
                     SAGEVMTFDIPAIIEILSRTMTLLPGDIIATGTPSGVGFARKPPVFLRPGDVVESDIA
                     GIGTLRNRFVAL"
     gene            complement(1950..2666)
                     /locus_tag="PK829_07250"
     CDS             complement(1950..2666)
                     /locus_tag="PK829_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41048.1"
                     /translation="MNSFEPIPWRGFRALDKLIFVAVPLAAVVIAVVPLLDPLLFRRV
                     ARLFGGRPLVAWLASGGAWIIAGSLMALLAVYVLWRRRSLVGNKALWFSSGCPACGEH
                     ELVRVSRHFSDRFYNLAAVPAYRYACRNCTWRGLRIARRDHLLNREEELEAALLRFDP
                     DARSAESDSWAVLSEAAAGPRHAESVFHDSGDVDWDEPDDPAQAYANGTDPHPSNHES
                     DEPPGDLDWIWRRPSGTDQI"
     gene            complement(2830..4647)
                     /locus_tag="PK829_07255"
     CDS             complement(2830..4647)
                     /locus_tag="PK829_07255"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU41049.1"
                     /translation="MTDRPNAAGTLIHRAGLALVLCCGLALLAACGQGGTETTATPTA
                     APEPTAAPSDVEPTRGQATVSNIDIQIMESFPVQVNVVASGNLPDGCTQIDEVISQRT
                     DEVFRVAITTLRQPSAVCTQALVPFEEVVPLDVAGLAAGTYEVSVNGISDTFTLDVDN
                     SLTESLGTPTTGAGAGTTPGISGFVWHDLCAQTGGTADPATTLAQGCVAGSDGTTIQA
                     DGVRDLSEPGIPGVTISLLAGDCTTATPGEEVTATTDESGAYRFEDVSADTYCVFLDT
                     ASEANATALGDGGVITSPLNNGVPTNSVTVTLAEGEALADVDFGFDFRFLPVAEAPAD
                     CTNSFEFVLDLTVPDDTVFPPGAEFEAGWRLRNNGTCPWDNYAVAFVNGDAMGITGTV
                     PIEDVVAPGQSADVAVTLTAPAAPGTYRSNWQLADADGTVFGINGQAEDAFWVQIVVE
                     EGAAAAGPPAPGSASISGVVWEDLCFLTNGTASRGCVETEEGSGFFRGNGTFDTNEAA
                     IPGVTLVLGRGACPPGGIIAVADRLATAVSDADGLYRFEGLDADIYCVSIDALSPENV
                     DLLIPGNWTWPAPGTGRQGIRLAGGEARESVDFGWDFQD"
     gene            complement(4644..>5700)
                     /locus_tag="PK829_07260"
     CDS             complement(4644..>5700)
                     /locus_tag="PK829_07260"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU41050.1"
                     /translation="LLRARLVPVEPERAGQMLRAILVFGGLAAAAWLLLALGLSRRRP
                     AAVVATLAVALLFADLYGLGRGVEIDWNDPTPGFAAGSPGLAFLRADPGIHRLDIATG
                     AWQPNMPMIASLYAARGVYNPLELANYNVYMGAVGYRGSPQYNVLGIKYVIGGKKEPP
                     GDTTFLVPVFDADPAVTVYLNTLALPRAMILFNSQVVADHDAAFAATHDAAFDPARVV
                     VLEGGQALAAEPGQATITIDRYGPNEAAFTTETDRAAYFFLSDVYHPDWQATVDGRPA
                     TIEVANYAFRAVYLEPGQHTVEMRFRPAGWAAGVAATAGALVTLVALAIICIRSNGAK
                     AAFPGPFAPDSSEEKEQ"
BASE COUNT          986 a         1806 c         1942 g          966 t
ORIGIN      
        1 tagccgcgga tgcggccgta gggcacgatg acgccgccct tgttgtaacc gatgacttcg
       61 ccctcgaaga tttggccgct ggccaacagg gcttccgcct caatccagtc ctggttcaac
      121 cgcgccaggc gaatagacac gaccagcgcc tccgagtctt ctgtctggac gacatagacc
      181 ggcacttcgt cgttgacctt cagcgcctcg cgctcttctg gttccagctg ggccaggtcc
      241 gactgcggca caatcccatc ccgcttcagg cccaaatcaa tgatcaggcc ctgcggggtg
      301 gtggcgacaa tcacgcccgt gcggatatcg cccacttgcg gcatgtcgta ggagtgctgg
      361 tccagcaggt cttcaaggga attcatcgat tcgtcagaca tagatctagt cctaaaccgg
      421 ctagcgattt agcgggttgc aggttccaat tcgacaataa aaaacggcct atcctggcga
      481 ctggtcgcta caggtggccg agtcaacaga cgctatcctc aacggataat gccgggcggc
      541 gcgctatgcc aaaggcacgc agcttagttg agttccaaca gggaaacctc cagatttacg
      601 cacgcgtcgc atcttacggt ttcttcgatt gtctcttgcc gtccaaccct aacccactca
      661 gccaaactcc aattatatca agagccaaga ctatgtcaat gtgcctgctg cccgaccagg
      721 gcttttccac agtcgccgat caatcgaaga cctgacaggt gggcgatcaa actctctctg
      781 gtgcaaagga aacttcaggc cgcccacctg tcaggtcagc gcactttagc gcgtctcagt
      841 cgtcgccagt tgctcaaaga cttgaccaaa gcctctgtgg cgcgaatgct cttttgggcc
      901 gcttatctga ccggtcaggt cgttgggcaa ctggcgattt acagggccac aaagcgattg
      961 cggagcgttc cgataccggc aatgtcgctc tcaactacgt cgcccggccg caagaacacg
     1021 ggcggcttac gggcaaagcc gacgccgctg ggcgtgcccg tggcgatgat gtcgcccggc
     1081 agcagcgtca tcgtgcgcga caggatttca atgatggccg ggatgtcaaa cgtcatcact
     1141 tccccggcgc tgtcctggcg cagttcgcca ttgacgcggc aggtaatgcg cagcgcgtgg
     1201 gggtctgcca cggcgtcggc ggtgacgatc cacggcccga tggggcagca gccgtccagg
     1261 ctcttgccga tgtggaactg cttgtgcagg ttttgcaggt cgcgggcggt cacgtcgttg
     1321 agaacagtat agccgaagac ggccgccagg gcctcggcgc ggttggcgcg gcgggtgggc
     1381 cggccgatga tgaaggccaa ctcgacctcg tagtccatct gcgccgagac agcgtcgtcg
     1441 atggcgatgt cgtcataggg gccgctgatg gtcgtcgtcg ccttggtgaa gatgactggg
     1501 tactcggtgg tgacgacctg ttcgccgcgg gcctcatacg attcgcgggc gtgctcggcg
     1561 tagttcttgc ccaggcagat gacattgcgg cgcggtatgg ggatgggggc cagcagacgg
     1621 gcgccggcca ggggcagcgc gtcgctggcc gtcttggcca gggcgcgaac gcgggccagc
     1681 gccgccgcgc cgccgtcgat gagggtcagc aggtcgggtg ctacgggcga tagatcgaca
     1741 atggcctcgc cgcgcagcaa gccgggggtg gggtggccgt ggtggtcgta ggtgagaagg
     1801 ttcaagagtt ctcctgtgcg ggcagtgggg tgataactgt tcgcgtgagg tttagccggc
     1861 ggagcgcttc gcggccgtct ctcgccaaac ctggttcatc cccttggaga gcttcaaggg
     1921 gctgtgcgcg tgtgcaaagt gggcgatggc tagatctgat ccgtgccgct cggacggcgc
     1981 caaatccaat ccaggtcacc gggcggttcg tcgctctcgt gattggacgg gtgggggtct
     2041 gtgccattgg cgtaggcctg ggcggggtcg tccggctcgt cccagtcgac gtcaccagag
     2101 tcgtggaaga cggactcggc gtggcgcggg ccagcggcgg cctcgctgag gacggcccaa
     2161 gagtcgctct cggccgaacg cgcgtcgggg tcgaagcgca acagcgccgc ttccagctcc
     2221 tcctcgcggt tgagcaagtg gtcgcggcgg gcgatgcgca gaccgcgcca ggtgcagttg
     2281 cggcaggcgt agcgataagc gggcaccgcc gccagattat agaagcggtc gctgaaatga
     2341 cgcgagacgc gcacgagttc atgttcaccg caggccgggc agccgctgct gaaccagagc
     2401 gccttgttgc caaccaggct acggcgtcgc cacaacacat acacagccaa tagcgccatc
     2461 aggctgccgg cgatgatcca cgcgccgccc gacgccagcc aggccacgag aggtctgccg
     2521 ccaaacagcc gcgccacccg ccgaaagagc agcgggtcga gaagcggcac aacggcgatg
     2581 acgacagccg ccagcggcac ggcaacaaag atcagtttgt cgagggcacg aaagccgcgc
     2641 cacgggattg gctcaaaact attcactact ccaaactccg acggaaaatt cccaccatca
     2701 ctcatcgtat gaggcctcgc ttacgttatt gcttacagaa ctgtctcggc cccagccggg
     2761 cagtggcgag tagggccgga gtggccggcc cataactcgc cgctgcccgg ccgggcgcgt
     2821 cgcggccggc tagtcctgga agtcccagcc gaaatcgacc gactcgcgtg cttcgccgcc
     2881 ggccagacga atgccctggc ggccagtgcc cggcgcgggc cacgtccagt tgccggggat
     2941 cagcagatcg acgttctccg ggctgagggc gtcgatggac acgcagtaga tgtcggcgtc
     3001 gaggccctcg aagcggtaga ggccatcggc gtcgctgacg gcggtggcca gccggtcggc
     3061 cacggcgata atgccgcccg gcgggcatgc gccccggccg agcaccagtg tcacgccggg
     3121 aatggcggcc tcgttggtgt cgaacgtgcc gttgccccgg aagaagccgc tgccctcctc
     3181 cgtctctacg cagccgcgcg aggccgtgcc gttggtcagg aagcatagat cttcccagac
     3241 gacgccgctg atggacgccg accccggcgc gggcgggcca gccgccgcgg cgccctcttc
     3301 gacgacgatc tgcacccaga aggcgtcttc agcctggccg ttgatgccga agacggtgcc
     3361 gtcggcatcg gccaactgcc agttgctgcg ataggtgcca ggtgccgctg gcgcagtcag
     3421 ggtgacggcg acatcggccg attgccccgg ggcgacgaca tcctcgatgg gcactgtgcc
     3481 ggtgatgccc atcgcatcgc cattgacaaa ggccacggcg tagttgtccc atgggcacgt
     3541 gccattgttg cgcagccgcc agccggcctc gaactccgcg ccgggcggga agaccgtgtc
     3601 gtcgggcacg gtcaggtcaa ggacgaactc gaagctgttg gtgcagtcag ccggcgcttc
     3661 ggccacaggc agaaagcgga agtcgaagcc aaaatcgacg tcggccagcg cctcgccctc
     3721 ggccagggtc acggtgacgc tattcgttgg cacgccgtta ttgagcggtg aggtgatgac
     3781 gccgccgtct cccaacgccg tggcgttggc ctcggaggcc gtgtccagga aaacgcagta
     3841 ggtgtcggcg ctgacatcct caaagcgata tgctccggac tcatccgtcg tcgcggtgac
     3901 ctcttcgccg ggcgtggcgg tagtacagtc gccggccagg agactgatgg tgacgccggg
     3961 gatgcccggt tcgctgaggt cgcgcacgcc gtcagcctgg atggtcgtgc cgtctgagcc
     4021 ggccacacag ccctgggcca gggttgtcgc cggatcggcc gtcccgcccg tctgcgcaca
     4081 caggtcatgc cagacgaagc cgctaatgcc gggcgttgtg cccgcgcccg cgcctgtcgt
     4141 tggtgtgccc aggctttcgg tcagggaatt gtccacgtct agagtgaagg tatcgctgat
     4201 gccgttgacc gagacctcat aggttccggc ggccagcccg gcgacgtcaa gcggcacgac
     4261 ctcttcaaac ggcacgagcg cctgggtgca gacggccgac ggttggcgca gcgtcgtgat
     4321 ggccacacgg aagacttcgt ccgtccgctg gctgatgacc tcatcgattt gggtgcagcc
     4381 atcgggcaaa ttgccgctgg caacgacgtt cacctggacg gggaacgact ccatgatttg
     4441 aatgtcgatg ttgctgaccg tggcctggcc acgggtcggc tcgacgtcgc tgggggcggc
     4501 ggtgggttca ggggcggcgg tcggcgtggc cgtcgtctcc gttcccccct ggccacaggc
     4561 ggccaggagc gccagcccac agcagagcac aagggccagg cctgctcgat ggatgagcgt
     4621 gccggcggcg ttgggtctgt ctgtcattgt tctttctcct ccgaagaatc gggcgcgaac
     4681 gggccgggaa aagcggcctt cgcgccatta gacctgatgc agatgatagc cagtgcgacc
     4741 agggtgacaa gcgcgccggc tgtggcggcc acgccggcgg cccagcccgc cggccggaag
     4801 cgcatttcga ccgtgtgttg gcccggttcc aggtagacgg cgcgaaaggc gtagttggcc
     4861 acttcgatgg ttgccgggcg gccgtccacg gtcgcctgcc agtcggggtg gtaaacgtcg
     4921 ctcaggaaga aataggccgc ccggtcggtc tcagtcgtga aggcggcttc attcggccca
     4981 tagcggtcga tggtgatggt cgcctggccc ggctcagccg ccagcgcctg gccgccctcc
     5041 agcacgacga cgcgcgccgg gtcgaaggcg gcgtcgtggg tggcggcaaa ggcggcatcg
     5101 tggtcggcca cgacctggct gttgaacagg atcatggccc gcggcagcgc cagcgtattg
     5161 aggtagacgg tcaccgccgg gtcggcgtcg aacacgggca cgagaaaggt cgtgtcgccg
     5221 ggcggctcct tcttgccgcc aatgacgtac ttgatgccca ggacgttgta ctgcggcgag
     5281 ccgcggtagc cgacggcacc catgtagacg ttgtagttgg ccagttccag cgggttatag
     5341 acaccacgag cggcatagag gctcgcgatc atgggcatgt tcggctgcca cgcgccggtg
     5401 gcaatgtcga ggcggtggat gccggggtcg gcgcgcagaa aggccagccc cggcgacccg
     5461 gcggcaaagc ccggcgtcgg gtcgttccag tcgatctcca cgccgcgccc caggccgtag
     5521 aggtcggcga agagcagggc gacggccagc gtggccacga ccgccgcggg ccgccgtcgg
     5581 ctcagcccca aggccagcag cagccaggcg gccgcggcca gcccgccaaa gacgaggatc
     5641 gcccgcagca tctgcccggc ccgctccggt tccaccggaa ccaggcgcgc ccgaaggagc
//
LOCUS       DAOSMQ010000178         5695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14322_length_5695_cov_5.298404, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000178 DAOSMQ010000000
VERSION     DAOSMQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14322_length_5695_cov_5.298404"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK829_07265"
     CDS             <1..299
                     /locus_tag="PK829_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOU41051.1"
                     /translation="ADRDRTERLFDFFYRIEIYTPAAKRQYGYYVMPILHGDRLIGRV
                     DPKMDRQTGQLIVQAVHWQPEATPDKDTRTAVAGAIESLGQFLGASSVRYLLDT"
     gene            424..1332
                     /locus_tag="PK829_07270"
     CDS             424..1332
                     /locus_tag="PK829_07270"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOU41052.1"
                     /translation="MTTWPEATVMRLLAAAAIFLVGVVYMRGWRQMARERLRLRRPLV
                     EPPPTRALWQFVAGLALLAVALISPLGYLATQYFSARIVQHMLIVASIPSLLMLSNPV
                     VPLAYGLPARQRDALLVALRRPAAASSGHLRRAVHWATSPGVTLLTFLCVCWFWYDPT
                     FHGATLRHAWVHAIELLSLFSVGLLNWWHITAAWPQTHGTMHPMVRIVYAFVSIWPVK
                     LVGLALLFFPEMNYGYPATFQFSGLNINDYNFGAMIAWIVSGLAYAVAAIVLARQWLG
                     EEADKPALPESAWATDESMNIPGLKR"
     gene            complement(1400..2008)
                     /locus_tag="PK829_07275"
     CDS             complement(1400..2008)
                     /locus_tag="PK829_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41053.1"
                     /translation="MISYRVRRLHAPGEAARRYHLFDRADQLLLVADQGSAWLPNDGT
                     PQVRFARPDGERVATMDLPRPADRRPEKQHNYAIIYEHAVYALITAPIRPPSGAAAPL
                     SAAGSIVWPFTRLLIEVEGHRWLALRWPEGAGPLLVLYDAGNDGVSLSADPESSDLPE
                     PLGLLEVGTGDYDFDLTLPAGRLEQGPLLALALVYLLDQAGK"
     gene            complement(2144..4081)
                     /locus_tag="PK829_07280"
     CDS             complement(2144..4081)
                     /locus_tag="PK829_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41054.1"
                     /translation="MAPELHPRLRSLGFLVVLLLAGLAIASRAVAQTWPTRVLAYVDG
                     DNIDGNFPHNDDSVTTDWEEFEQPAVSPNGQTIAYVHTFLSSGVWFRNTEIVRINADG
                     SGAQRLTDNDLWESWPTWSPDGTRLAFAAGDLYVMPVTAAGGGTPVQLTTGTLYPENL
                     AWSPDGTRLAFNSRPTCPQAPECEYAAELYVVTVAGGQLTRLTDNDQYDGDPTWSPDG
                     AQLAYESRPTPNGDRHLYRITAAGGPATPIVTDADSADPAWSPDGARVAYATRQGIKT
                     VRPDGTAQLAVTEDTGDYVFDPLWTTDGRRLTFLNLEFFSCGHSGCYKRNLLRVDVDS
                     GHIELLDEVGISYLPEPLMWSATDHLIAYIPSPTSFLYARDLSASSPGPARQLTPDIP
                     INADPAWSPDGRRLVFNSRRDFNYNIHVINADGTGRQALTTHRGNDWNPSWSSDEEIA
                     FTSNRDGNWELYKMNEDGRNQTNLTRTPAIAEDDATWSPSGAQLAYARQENGNWDIYV
                     MNADGSGVTRLTTHAADDRNPTWSANAARIAFETNRDGNDEIYILTVDGPPGNEVNWT
                     RSPTDQREPAWSPDGTEIAFVSVTTTRGGWAPAALVTMPVANPSAGQILISDLTPPVQ
                     PAWRLVAYDFPPRAWVPVIRH"
     gene            4238..>5695
                     /gene="dnaE"
                     /locus_tag="PK829_07285"
     CDS             4238..>5695
                     /gene="dnaE"
                     /locus_tag="PK829_07285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU41055.1"
                     /translation="MSASASEHEPFVHLHVHTEFSLLDGLSRIKHLAARAKALNQPAL
                     ALTDHGTMFGVIPFYEACVAAGVKPIIGIETYVAARRMTDHDPQLDRDRTHLLLLAEN
                     HTGYLNLLQLASRAQLDGFYYKPRIDLDLLAQHSEGLICTTGCMAADIPRAIVAGDLK
                     KAHTQLGKYLDIFGSERYFIELQEHSIPELTALNKTYVEEFQARYNLRFVATNDVHYT
                     TPEEASPHDVLLCVQTGATVNESRRLRFSDKGYYLKSRAEMAELFGHVPGALDNSLLI
                     AEMCNLDLSSKGYHLPIFPVPDGYDTASYLRELCEQGLAWRYGAERAHADPVVRARLE
                     HELAVIHRMGFDAYFLIVWDLCQFARQADIWWNVRGSGAGSVVAYSLGITGIDPIANG
                     LIFERFLNPGRVTMPDIDLDYPDDRRHEMVQYAVQKYGQDKVAQIITFGTLGARAAIR
                     DVGRALDVPLPEVDAIARLIPAIPGKPAKIVNVLDK"
BASE COUNT          969 a         1847 c         1860 g         1019 t
ORIGIN      
        1 tcgccgaccg cgaccggacg gagcggctgt tcgacttctt ctaccgcatc gagatctaca
       61 cccccgcggc caaacggcag tacggctatt acgtcatgcc cattctccac ggtgaccggc
      121 tcatcggccg tgttgatccg aagatggatc gccagacggg ccagctgatc gtgcaagccg
      181 tccattggca accggaggca acgccagata aggatacacg gacggccgtg gccggggcga
      241 ttgagtccct ggggcagttc ctgggggcaa gttccgtgcg ttatctgctg gatacatgag
      301 cgcttttggc ccattctcga aaagaaaccg gtgggcaaaa gtttaggaag ccactgatcg
      361 cgtagattaa tatgcctcat gtagtggttt cctttctcct tcctggcgct acaatctagc
      421 ctgatgacca cctggcccga agcgacggtg atgcgcctgt tggcggcggc ggcgatcttc
      481 ctcgttggcg ttgtctacat gcgcggctgg cggcaaatgg cacgcgagcg cctgcggctg
      541 cgccggccgc tggtcgagcc gccgcccacg cgggcgttgt ggcagttcgt ggccgggctg
      601 gcgctgctgg ccgtggccct catttcgccg ctgggctatc tggcgacgca gtacttcagc
      661 gcccgcattg tccagcacat gctcatcgtc gcctcgatcc ccagcctgct gatgctgtcg
      721 aacccggttg tgccgctggc ctacgggctg ccggcgcgcc aacgtgacgc gctgctggtc
      781 gcgctgcgcc ggccggccgc cgcctcgtcc ggccacctgc gccgtgccgt ccattgggcc
      841 acctcgcccg gcgtgacatt gctgactttt ctatgcgttt gctggttctg gtatgacccc
      901 acgttccacg gggcgacgct gcgccacgcc tgggtccacg ccatcgaact gctcagcctc
      961 ttcagcgtcg gcctgctcaa ctggtggcac atcaccgccg cctggcccca gacgcacggg
     1021 acgatgcacc cgatggtgcg catcgtctac gccttcgtca gcatctggcc ggtgaagctg
     1081 gtcgggctgg cgctgctctt cttcccggag atgaactacg gctacccggc cacgttccag
     1141 ttcagcggcc tgaatatcaa cgactacaac ttcggggcga tgatcgcctg gatcgtcagc
     1201 gggttggcct acgccgtcgc cgccatcgtg ctggcgcggc agtggttggg cgaggaagcc
     1261 gacaagccgg cgctgccgga gtcggcctgg gccacggacg agtcgatgaa tatcccgggg
     1321 ctgaagcggt aagaataatt aggaattagg aatcagcaga gacgaccgag cattcctctt
     1381 cattcctaat tcctaattct catttccccg cctggtcgag caaatagacc aacgccagcg
     1441 ccaacaacgg cccctgctcc agccggccgg cgggcagcgt caggtcgaag tcgtagtcgc
     1501 ccgtgcccac ttccagcagg ccgagcggct ccggcaggtc gctcgattcg gggtcggccg
     1561 agagcgatac gccgtcgttg ccggcatcgt agagcaccag cagcggcccc gccccctctg
     1621 gccaacgcaa cgccagccag cgatgccctt ccacctcgat cagcagccgc gtgaacggcc
     1681 agacgatgct gcccgcggcc gataggggcg cggcggcgcc gctgggcggc cggatgggcg
     1741 cggtgatgag ggcatagacg gcgtgctcat agatgatggc gtagttgtgc tgcttctccg
     1801 ggcggcggtc ggccgggcgg ggcaggtcca tggtcgccac gcgctcaccg tcggggcggg
     1861 caaagcgcac ctggggcgtg ccgtcgttgg gcagccaggc gctgccctgg tcggccacga
     1921 ggaggagttg gtcggcgcgg tcgaagaggt gatagcgccg cgccgcttcg ccgggcgcgt
     1981 gcaagcgccg gactcggtag gagatcatag gcgcagattg tacacgattt ccctgcgggg
     2041 aacacaggtc agggggcgag ggtcgggcag cagttgtgag taggccgcgg ccaattcctg
     2101 acaactggct gctgatcatt ggccgctcac cacgggctgc tggttagtgg cggatcacag
     2161 gcacccaggc gcgcggcggg aagtcatagg caaccagacg ccaggccggc tggacgggcg
     2221 gcgttagatc agatatgaga atttggcccg cggaggggtt ggcaaccggc attgttacca
     2281 gcgccgcggg cgcccagccg ccgcgggtgg tggtgacgga gacgaaggcg atttcagttc
     2341 cgtcgggcga ccaggccggc tcgcgttggt cagtcgggga gcgcgtccaa ttcacctcgt
     2401 tgcccggcgg cccatccacc gtcaggatgt agatctcgtc gttgccgtcg cggttggtct
     2461 cgaaggcgat gcgcgcggcg ttggccgacc aggtcgggtt acggtcgtcc gcggcgtggg
     2521 tcgtcaggcg ggttacgccg gaaccgtcgg cgttcatgac gtaaatgtcc cagttgccgt
     2581 tctcctgacg ggcatatgcc aactgcgccc cgctgggcga ccaggtggca tcgtcttcgg
     2641 cgatcgccgg cgtccgggtc aggttggtct ggttgcggcc gtcctcgttc attttgtaaa
     2701 gttcccagtt gccgtcgcgg ttggaggtga aggcaatctc ctcgtccgat gaccaactgg
     2761 ggttccagtc attgccgcga tgggtggtca atgcctggcg cccggtgccg tcggcattga
     2821 tgacgtggat gttgtaattg aagtcgcgcc ggctgttaaa gaccagccgg cgaccgtcag
     2881 gcgaccaggc tgggtcggcg ttgatcggta tgtcgggagt taattgccgg gccgggccgg
     2941 gggaagaagc cgagaggtcg cgcgcgtaga gaaaggaggt ggggctgggg atataggcga
     3001 tcagatgatc ggtggctgac cacatcaacg gctcggggag gtagctgatg cctacctcgt
     3061 ccagcagttc aatatgcccc gaatccacat ctacccgcaa caggttcctc ttatagcacc
     3121 cgctatgccc gcatgagaag aattccaggt tgagaaaggt cagacgccgg ccgtcggtcg
     3181 tccacagggg atcgaacaca tagtcgcctg tatcttcagt aaccgcaagt tgggccgtgc
     3241 catccggtcg aacggttttg attccttgcc gagtggcgta ggcgacgcgc gcgccgtcgg
     3301 gcgaccaggc cgggtcggcg ctatcagcat cggtcactat gggcgtcgcc gggccgccgg
     3361 cggcggtgat ccggtagaga tggcggtcgc cgttcggggt tgggcgggat tcataggcta
     3421 attgagcgcc gtcgggcgac caggtcgggt cgccgtcgta ctggtcgttg tcggtcagcc
     3481 tcgtcagttg gccgccggcg acagtcacga catagagctc ggccgcgtat tcgcactctg
     3541 gcgcttgcgg gcaggtcggc cggctgttga acgccaatcg cgtgccgtcg ggcgaccagg
     3601 cgaggttttc cgggtagagc gtccccgtgg tcagttgcac gggcgtgccg ccgcccgcgg
     3661 cagtgacggg catcacgtag agatcaccgg cggcgaaggc cagccgcgtc ccatcgggcg
     3721 accaggtcgg ccaggactcc cacagatcgt tgtcggtcag acgctgggct ccgctgccgt
     3781 cggcgttgat gcggacaatc tccgtgttcc tgaaccacac gccggaggaa aggaacgtgt
     3841 gcacgtaggc gatcgtctgg ccattgggcg acacggccgg ctgctcgaat tcctcccaat
     3901 cagtcgtcac gctatcgtcg ttgtggggaa aattgccgtc aatattgtca ccgtcgacgt
     3961 aggccaacac ccgcgtaggc catgtctggg ccacggcgcg gctggcgatc gccagcccgg
     4021 ccagcagcag aacaaccaga aagcccagtg agcgcaaacg ggggtgaagt tctggcgcca
     4081 tcgatttcct cctcaacacg cgacaacagg gtggcgacgc aacgacttga tcgcattgta
     4141 ctctccttta accggcaagc aaccactacc aacgaccgcc cttaaaatct cgccttcttt
     4201 aagctccgat ccgaacagtt gtgttatact cccgcccatg tcggcgtccg ccagcgaaca
     4261 cgaaccgttt gtccatctgc acgtccacac ggagttctcc ctgctcgatg gcctgagccg
     4321 catcaagcac ctggcggcgc gggccaaggc gctcaaccag ccggcgctgg ccctgaccga
     4381 ccacgggacg atgttcggcg tcatcccctt ttacgaggcc tgcgtcgccg cgggggtcaa
     4441 gccgatcatc ggcatcgaaa cctacgttgc cgcccggcgc atgaccgacc acgacccgca
     4501 gcttgaccgc gaccgcaccc acctgctgct gctggccgag aaccatactg gctacctgaa
     4561 cctgctgcaa ctggccagcc gcgcgcaact agatggcttc tactacaagc cacgcatcga
     4621 ccttgacctg ttggcccagc acagtgaggg cctcatctgc accaccggct gcatggccgc
     4681 cgacatcccg cgggccatcg ttgccggcga cctgaagaaa gcccacacgc agctgggcaa
     4741 gtacctcgat atcttcggct cggagcgcta cttcatcgag ttgcaggaac atagcatccc
     4801 agaactgacg gcgctgaaca agacctacgt cgaggagttc caggcgcgct acaacctgcg
     4861 cttcgtggcc accaacgacg tccactacac cacgccggag gaggcctcgc cccacgacgt
     4921 gctgctgtgc gtccagaccg gggcgacggt caacgagtcg cgccggctgc gcttcagcga
     4981 caagggctac tacctcaagt cgcgggccga gatggccgag ctgttcggcc acgtccccgg
     5041 cgcgctggac aacagcctgc tcatcgccga gatgtgcaac ctcgacctga gcagcaaggg
     5101 ctaccacctg cccatcttcc ctgtgccgga tgggtacgac accgccagct acctgcgcga
     5161 actgtgcgag caggggctgg cctggcgcta tggcgcagag cgcgcccacg ccgacccggt
     5221 ggtacgggcg cggctggagc atgagctggc cgtcatccac cgcatgggct tcgacgccta
     5281 cttcctgatc gtctgggacc tgtgccagtt tgcccggcag gcggacatct ggtggaacgt
     5341 gcgcggcagc ggcgccggct cggtggtggc ctactcgctg ggcatcaccg gcatcgaccc
     5401 catcgccaac ggcctgatct tcgagcgctt cctcaacccc ggccgtgtca ccatgcccga
     5461 catcgacctg gactaccccg acgaccgccg gcacgagatg gtgcaatacg ccgtgcagaa
     5521 gtacggccag gacaaagtcg cccagatcat caccttcggg acgctggggg cgcgggcggc
     5581 catccgcgac gtcggccgcg ccctcgacgt gcccttgccg gaggtagacg ccatcgcccg
     5641 gctcatcccg gccatccccg gcaagccggc caagatcgtc aacgtgctgg acaag
//
LOCUS       DAOSMQ010000179         5677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14378_length_5677_cov_4.536286, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000179 DAOSMQ010000000
VERSION     DAOSMQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14378_length_5677_cov_4.536286"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PK829_07290"
     CDS             <1..710
                     /locus_tag="PK829_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41056.1"
                     /translation="GPWWCMPDPEPDFSPCEAILHAEPAGELRQPTAEELAILQAAPT
                     PTTEADVATAAPTPTPRPIEGSDPQTIILNAGCGSCHKIGAIGEAHKVGPDLSAIGLT
                     AGGRVPGFTAEQYIRQSVLEPNAYLAPACPNSPCLPSIMPRDYATRLSPAQQDTLVAF
                     LLTQQGEEPTPAAVGAGTGMATPLPKPAVVAKVAPPSNRISSLTVAQIVLALVVVVVS
                     AYALFRDRSGGRPESDE"
     gene            complement(715..1182)
                     /gene="smpB"
                     /locus_tag="PK829_07295"
     CDS             complement(715..1182)
                     /gene="smpB"
                     /locus_tag="PK829_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOU41057.1"
                     /translation="MATSGNVKVIFNNKRANFDYELLERFEAGLVLTGTEIKSIRANH
                     VSLQRSYVQPRGDELWLLEANISPYEHGNRENHEPTRPRKLLLHRREINRILTNLAQK
                     GLTVVPTRLYLKGGRAKVEVALARGKRKFDKRADIARRDSDRQVERALREKYR"
     gene            complement(1197..2642)
                     /locus_tag="PK829_07300"
     CDS             complement(1197..2642)
                     /locus_tag="PK829_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU41058.1"
                     /translation="MSYDPSNLERGSAGRRSPGLLLIGLVAVLLALATGALGYVIGRG
                     TSSASPAARPTTAAVAQFDTPTAAPPTTAPPTAAPPTAVPPTAVPPTDTPTPPPTATA
                     TAAPLTPSPTPAISLDGQSPLAGADLGTFYQAWDIVAGEYDGALPPAEELLQSAIAGS
                     LEALGDDYTRYVSPDVAERTREDMNGAVEGIGAMVRENDEGLFEIVRPLDGQPADLAG
                     LKAGDVVIEVDGQSALEMSFDEVILLVRGPAGTSVTLTVMREGEPEPLEFTIVRARFE
                     VAVVEYEMLPVEMTGGAAIGYIHLTEFGRTAEEKLLEALTAILAQQPAGLVFDLRDNP
                     GGFLDQSVAVADAFLPAGVALFERNLRGLDETFSTGDGDVAESIPLVVLVNAGSASAA
                     EIVAGALQDRGRAVLVGETTFGKGSVQQVHTLSDGGELRVTVARWYTPNNHSIQGEGI
                     TPDITVASPENLGGPDDGQLQRAIDFLLNGE"
     gene            complement(2665..3870)
                     /locus_tag="PK829_07305"
     CDS             complement(2665..3870)
                     /locus_tag="PK829_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU41059.1"
                     /translation="MTNRLRSGLTIFMLLILVASAFAAGFLARELVDGRNDATLAHAG
                     QDEETALFQEAWSLIEQNFLGALPESRQVTYGAIRGSLATLNDPYTVFIEPAARDVER
                     ERLQGSFGGIGAYITRSEETGAVTLEPIPGNPAEAAGILTGDVLLAVDGQAITAELTV
                     PEIIDLVKGEKGTTVTLTVRHPDAAEPVDINVVRADILLPSVAYQLLEADPTIGYIAL
                     SRFSGESGNEVGAALQDLLAQGATRLVLDLRQNGGGLRDAAVDVADHFLNSGPVLYLD
                     SQQEGETVFNATEETLAPDQPLVVLIDGGTASASEIVAGALSDRQRATLIGSKTFGKG
                     SVQLVFDLSDGSSVHVTSARWLTPNHRTLDGNGLEPDIPVAITQEDIDNGRDPVLERA
                     VQYLQESAP"
     gene            4113..>5677
                     /locus_tag="PK829_07310"
     CDS             4113..>5677
                     /locus_tag="PK829_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU41060.1"
                     /translation="MRSPGALRLVVLVLALSMIMLLAPAPARSQDEPNLPAQAEALLQ
                     AMTVPERIGQLFLVTFQGDRAPADSAIGELILTYHIGGVALLAENDNLTGYGDPAQTP
                     AQVRDLTANLQRLALLGFSAEPAPADEDGNVPPTAEPQPSAVPVPLLIATMQDGDSLP
                     RRNVMTGYTPVPNNMALGATWQPAYARRIGEVVGGELAATGINLLLGPSLDVLERPSP
                     LSAGDLGTNVFGGDPYWVGLMGRAYIEGVHAGAGQRLAVAARSFPGQGSSDRPVDVEV
                     PTVRRSLEQLKQIELAPFFSVTRQTPGAPETADALLATHIRYQGFQGNIRATTAPVSL
                     DPQALNSLMTLPEFAPWRQGGGVIVSDRLGLRSVRRFYDDTEREFPHRQVAKDALLAG
                     NDLLFLANFGVSTADDSVQLANITDTIAWFRERYETDPSFRQRADEAVRRILQLKLRL
                     YGGDISAANVIGEPVAEPPVQPVGAAAFFDIAEAAVTLLAPSRDELSARLATPPTLGN
                     RLVIFTDVQTAQP"
BASE COUNT          912 a         1978 c         1853 g          934 t
ORIGIN      
        1 gcgggccgtg gtggtgcatg cccgacccgg agccagactt ttccccctgc gaggccattc
       61 ttcacgccga gccggccggc gaactgcgcc agccgacagc cgaggaactg gccatcctgc
      121 aagccgcgcc cacgccgacg actgaggcgg atgtcgccac cgccgcgccc acgccgacgc
      181 cgcgccccat tgaaggcagc gacccacaga ccatcatcct caacgccggt tgcggctcct
      241 gccacaagat cggcgccatc ggcgaggccc acaaggttgg gccggacctg agcgccatcg
      301 ggctgacggc cggcgggcgc gtgcccggct tcactgccga gcagtacatc cgccagtcgg
      361 tgctggagcc aaacgcctac ctggctccgg cctgccccaa tagcccctgc ctgcccagca
      421 tcatgccccg cgactacgcc acgcgcctca gtcccgcgca gcaggacaca ctggtggcct
      481 ttctgctgac ccaacaaggg gaggagccga cgccggcggc cgtcggcgcg ggcacgggga
      541 tggccacgcc gctgcccaag ccggctgtcg tcgccaaggt cgcgccgccc agcaaccgca
      601 tctcgtcgct gacggtggcg cagatcgtcc tggcgctggt ggtcgtcgtc gtcagcgcct
      661 acgcgctctt ccgcgaccgg tcaggcggcc ggccggagag cgacgagtag cgccctagcg
      721 atacttctcg cgcagcgccc gctccacctg gcggtcagag tcgcggcggg cgatgtcggc
      781 ccgcttgtcg aacttgcgct tgccgcgggc cagagccacc tcgaccttgg cccggccacc
      841 cttcaggtac agccgcgtcg gcacgacggt cagccccttc tgggccagat tggtcaggat
      901 gcggttgatt tcccggcggt ggagcagtag cttgcgcggc cgtgtcggct cgtggttctc
      961 gcggttgccg tgctcatagg gagagatgtt ggcctccagc agccacaact catctcccct
     1021 gggctgcacg tagctgcgtt gcaggctgac gtggttggcg cggatggact taatctccgt
     1081 gcccgtcagc accagacccg cctcgaagcg ctccagcagc tcgtagtcga agttggcccg
     1141 cttgttattg aaaatgactt tgacgttgcc gcttgttgcc atatctcggc gctcacttac
     1201 tcgccattta gcaggaaatc gatcgcgcgc tgcaactggc catcgtctgg cccgcccagg
     1261 ttttccggcg atgcgacggt gatgtcgggg gtgatgccct cgccctgaat gctgtggtta
     1321 ttgggtgtgt accagcgcgc cacggtcacg cgcagttcgc cgccgtcgct cagcgtgtgc
     1381 acctgctgca ctgagccttt gccgaacgtc gtctcgccca ccagcacagc ccggccgcga
     1441 tcctggaggg ccccggcgac gatctcggcc gcgctggcgc tgccggcatt gaccaggacg
     1501 acgagcggga tgctctcggc cacgtcgccg tcgccggtgc tgaaggtctc gtccagcccg
     1561 cggaggttgc gctcgaacag ggccacaccc gccggcagaa aggcatcggc cacagccacc
     1621 gactgatcca ggaagccgcc ggggttgtcg cgcaggtcga acaccaaacc ggctggctgc
     1681 tgggccagga tggccgtcag cgcttccagc agcttctcct cggccgtgcg accgaactcg
     1741 gtcaggtgga tgtagccaat cgctgcgccg ccggtcattt ccaccggcag catctcatac
     1801 tcgacgaccg ccacctcgaa gcgggcgcgg acaatggtga actccagcgg ctccggctcg
     1861 ccctcgcgca tcaccgtcag cgtcacgctc gttcccgccg gcccgcgcac cagcaggatc
     1921 acctcgtcga aactcatctc cagcgccgac tgtccatcga cctcgatgac gacgtcgcca
     1981 gccttcagcc cggccaggtc ggccggttgg ccgtcgaggg gccgcacgat ctcgaacagg
     2041 ccctcgtcgt tctcgcgcac catcgcgccg atgccctcga ccgcgccgtt catgtcttcg
     2101 cgcgttcgct cggccacgtc gggagagaca tagcgcgtgt agtcgtcgcc cagcgcttcc
     2161 agcgagccgg cgatagccga ctgtagcagt tcctcggccg gcggcagcgc gccgtcgtac
     2221 tcgccagcga cgatgtccca ggcctggtag aacgtgccta agtcagctcc ggccaggggc
     2281 gattggccat cgaggctgat cgccggggtt ggcgaggggg tcaagggcgc ggccgtcgcc
     2341 gtcgccgtgg gtgggggcgt gggcgtatcc gttggcggca cggccgttgg cggcacggcc
     2401 gtcggtggcg cggccgttgg tggcgcggtc gttggcggcg cggccgtcgg cgtgtcgaat
     2461 tgggccaccg cggccgtcgt tggccgagcc gcgggcgaag ccgacgacgt gccccggcca
     2521 atgacgtagc caagcgcgcc cgtcgccagc gccaggagca cagcgaccag gccgatcaac
     2581 aaaagaccgg gcgagcgtcg cccggcggaa ccgcgttcga ggttagaagg gtcgtaggac
     2641 atagaatccg tgggcagcca ggagctaggg agcactctcc tgcaagtatt gcacggcgcg
     2701 ctccagcacc gggtcgcggc cgttgtcgat atcttcctgc gtaatggcca ccggaatgtc
     2761 cggctccagg ccattgccat ccagcgtgcg gtgattaggc gtcagccagc gcgccgaagt
     2821 cacatggacg gatgagccgt cgctgaggtc aaagacaagc tgtaccgatc ccttaccgaa
     2881 ggtcttgctg ccgatcagcg tggcccgctg ccggtcgctg agcgcgccgg cgacgatctc
     2941 cgaggcgctg gccgtgccgc cgtcaatgag gacaaccagc ggctggtcag gagccagggt
     3001 ctcctcggtg gcgttgaaca cggtctcgcc ttcctgctgg ctatccagat agagtaccgg
     3061 gccgctattg aggaagtggt cggccacgtc cacggccgcg tcgcgcaggc cgccgccgtt
     3121 ctggcgcagg tcaagcacca gccgcgtcgc cccctgcgcc agcaggtctt gcagcgccgc
     3181 gcccacttcg ttgccgctct cgccgctgaa acgggacaag gcaatgtagc cgatggtcgg
     3241 atccgcctcc agcagctggt aggccaccga cggcagcagg atatcagcgc ggacaacgtt
     3301 gatatccacc ggctcggccg cgtcggggtg gcgcacggtc agggtgacgg tcgtgccctt
     3361 ctcgcccttc accaagtcga tgatttccgg cacggtcagt tccgcggtaa tcgcctggcc
     3421 atcgacggcc agcagcacat cgccggtcag gatgccggcc gcttcagcgg gattgccggg
     3481 gatgggttct agagtaaccg cgcccgtttc ttccgagcgc gtgatgtagg cgccgatgcc
     3541 accgaaggag ccttgcagcc gttcgcgctc cacgtcgcgg gcggccggct cgatgaaaac
     3601 ggtgtaaggg tcgttgagcg tggccagcga gccacggatc gccccatagg tcacctggcg
     3661 cgactctggc aacgcgccca ggaagttctg ctcgataagc gaccaggcct cctggaagag
     3721 ggctgtttcc tcatcctgcc cggcgtgggc cagcgtcgcg tcgttgcggc catcgacaag
     3781 ctcgcgggcc aggaagccgg cggcgaaggc gctggcaacc aggatcaaga gcatgaaaat
     3841 agtcaatccg ctgcgaagac gattggtcat aagcgtgcac gcgttacgaa gcgcgaatga
     3901 tagcgatttt aacacaggtg atcggcgcgg caattggcgc tataatccgc ggtggtggct
     3961 gttagtaagg gttaatcgtt ctctgacgca aacccaaagc ggccactgtc gagttgccgg
     4021 gtgatcgcgc aaaacgccgc ttgcctcgta tcaccggccg ctcttgaggg cgttggggga
     4081 gcgtgatccc cggcgcaggt agtaagtcaa acatgagatc acctggcgcg ctgcgcctgg
     4141 tcgtgttggt tttggcgctg agcatgatca tgctgctggc gccggccccg gcccgcagcc
     4201 aagacgaacc aaacctgcca gcccaggccg aggccctctt gcaggccatg accgtcccgg
     4261 aacggatcgg ccagctgttt ctggtcacct ttcagggcga tcgcgccccg gccgatagcg
     4321 ccatcggcga gctgatcctg acctaccaca tcggcggcgt ggccttgctg gccgaaaatg
     4381 acaatctgac cggctatggc gatcccgcgc agacgccggc acaagtgcgc gacctgaccg
     4441 ccaacttaca gcgcctggcc ttgctaggct tcagcgccga gccggccccg gccgacgaag
     4501 acggcaacgt cccgcccaca gctgagccgc agccgtcggc cgtgcccgtc ccgctgctca
     4561 tcgccaccat gcaggacggc gacagcctgc cgcggcgcaa cgtcatgacc ggctacaccc
     4621 ccgtgcccaa caacatggcc ctgggggcga cgtggcagcc ggcctacgcc cgccgcattg
     4681 gtgaggtcgt cggcggcgaa ctggccgcca cgggcattaa cctgctgctt ggcccgtcgc
     4741 tcgacgtgct ggagcggccg tcgcctctca gcgccggcga cctgggcaca aacgtatttg
     4801 gcggcgaccc ctactgggtc gggctgatgg gccgagccta catcgagggt gtccacgccg
     4861 gcgcgggaca gcggctggcc gtggccgcgc gcagcttccc cggccagggg agcagcgacc
     4921 ggcccgtgga cgtggaagtg ccgaccgtgc gccgttcgct ggagcaactg aagcaaatcg
     4981 aattggcgcc cttcttcagc gtcacccggc aaacgcccgg cgcgccggag acagccgacg
     5041 ccctgttggc cacccacatc cgctatcagg gcttccaggg caacattcgc gccaccactg
     5101 cgcctgtcag ccttgatccc caggcgctga actcactcat gaccctgccc gaatttgcac
     5161 cctggcgaca gggcggcggc gtcatcgtct ccgaccggct gggtctgcgc agtgtgcggc
     5221 gcttttacga tgacaccgag cgcgagtttc cccatcgcca ggtggccaag gacgccctgc
     5281 tggccggcaa cgatttgctc tttctggcca actttggcgt gagcacagcc gatgactccg
     5341 tccagctggc caacatcacg gacaccatcg cctggttccg cgaacgctat gagaccgacc
     5401 ccagcttccg ccagcgcgcc gatgaggccg tgcggcgcat cttgcagcta aagctacggc
     5461 tctatggggg tgacatctcc gccgccaacg tcatcggcga gccagtcgca gagccgcctg
     5521 tccagcccgt gggcgcggcg gccttcttcg acattgccga ggcggccgtc actctgctgg
     5581 cccccagccg cgacgagttg agcgcccgtc tggccacgcc gccaacgctg ggcaatcgcc
     5641 tggtgatctt cactgatgtc cagacggcgc agccctg
//
LOCUS       DAOSMQ010000180         5675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14388_length_5675_cov_6.067082, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000180 DAOSMQ010000000
VERSION     DAOSMQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14388_length_5675_cov_6.067082"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK829_07315"
     CDS             complement(<1..267)
                     /locus_tag="PK829_07315"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU41061.1"
                     /translation="MTEPVAPRRRLGNWGEGVAAVQLEAKGYAIVARNWRCAVGEIDL
                     VARDGETLVFVEVKTRRGRAYGAPEEALTPRKAQKLLQLGAQYVY"
     gene            complement(260..1519)
                     /locus_tag="PK829_07320"
     CDS             complement(260..1519)
                     /locus_tag="PK829_07320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU41062.1"
                     /translation="MINGVVRMVTLYKRHLEARGHEVTIFTLGEEQADDAGARIVRSP
                     GMRLGEHGYFIGLGYSQEAQRLLQTMEIVHCHHLLMSVEMAHRYAQCPIVYTNHTRYD
                     LYTGAYTPLPQPAADAIMRQLWPEFAGLADTVIAPSASVRQLLIDFGVSNPIVVIENG
                     IERESFLRPLRPRAKADFGVPDDAFLLLYTGRLAEEKNLAGLLAQFALAAGVMPALRL
                     AIAGKGPQEEELRRLVVERQLDERVHFVGVVPFDEVGDWLAAADAFVTASTSEVHPLT
                     VIEAMAAGKPIIAVQSPGISDSVESGVTGLIVPDVEGLSAAIAALAANPARARAMGEA
                     ARAASARFDIARMIDLTVALYDSLLAERPDLQREDAHGRWSRRTEKWSAMLEQLTQLV
                     RPSDDADSGPPRWWPANVLTARRERHD"
     gene            complement(1593..2117)
                     /locus_tag="PK829_07325"
     CDS             complement(1593..2117)
                     /locus_tag="PK829_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU41063.1"
                     /translation="MQPSIRTILATTEIFDNLSEAQLTLVAALCTPVTYRRGYIVLSE
                     NEASDEMYVIGRGGVEVLVNPNAVGDNTGAGEGGRPPLEPVVLSELRQGQVVGEVALV
                     DQGVRSATIRVSRDDTLLLRLRRDQLMRLGETYPVLGFKLMRNLAAELATKIRNTDWL
                     VRQYQLQLQENAPD"
     gene            complement(2136..2927)
                     /locus_tag="PK829_07330"
     CDS             complement(2136..2927)
                     /locus_tag="PK829_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41064.1"
                     /translation="MNDPWMPFLAAAVALVLLVFLQRWIHAHLHGVSLLLTGRAELAL
                     VVYAIILFPGVLLHEASHWLSATLLGVRTGGVSLLPRRQPDGTLQLGYVEYYRGRTLD
                     PLRESLIGGAPLIAGTAVILFIGYRVFGVTSLAAAVQTGDINALAAAMTELLATPAFA
                     LWLYLLFAIGNAMLPSKSDRHAWPAFLLWLAVLILVVAFVTRGRAVLDNLAGPVATFF
                     GYLAVALYIAIAVDVLFMAVIAVAEWLLGRARGMSVIYGQQGNEQ"
     gene            complement(2941..3696)
                     /gene="tpiA"
                     /locus_tag="PK829_07335"
     CDS             complement(2941..3696)
                     /gene="tpiA"
                     /locus_tag="PK829_07335"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU41065.1"
                     /translation="MRTPFVAGNWKMNKTADAAVEFVREIAADLNAINGIDIAVCPPF
                     IALPAVARALAGTRVRVGAQNMYYETSGAYTGEISPAMLQGLCQYVILGHSERRAYFG
                     ETDEGVNKKIFAALVHGLTPIVCVGESLEQNQAGQTQSFVSGQVRAAFAGCSPAQAAE
                     CVIAYEPIWAIGTGLSASAQQAEDIIRASVRDVLTEMFGEATAQAIRIQYGGSVTAEN
                     ISEFMRQPDIDGALVGGASLKTSFVHLVRGAVV"
     gene            complement(3906..>5675)
                     /locus_tag="PK829_07340"
     CDS             complement(3906..>5675)
                     /locus_tag="PK829_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU41066.1"
                     /translation="AFDIAAAPLAGQLLGRLLLVEDAATAYRLARDWPPGYAAVAPDG
                     VVVRGGGLVEVHGGGAGVLAQEARRREAATALETLHAALAEAEAAAAGRAAAIDRLQE
                     DVDGRAAEEQRLSRLEAEAAGRLTAARRELDRAQQQRDFAVRRRAERQTAIQSLQSRI
                     AQTEETLARQRERVAQTETALAAARAQLADLPVGEAEQQQQSLRQSVAAAQTIVAGRQ
                     AVVDSRRATLNQADGQLERLRRRVEALRGQLAEVESEDEADLIVAERRLVEVETELAP
                     LLASAESDRALLARLQADLSPRQRHAHELETQLTQTHIRQTQQENHIEALQERIRADL
                     GLVALSYDAEQTGATPLPMRDVVAELPVVAELPADIEENIHHYRGQLQRMGPINPDAP
                     AELASTEERFEFMTQQVGDLSETDARLRAVITELDELTSVAFARTVEQVNGIFDQTFR
                     QLFGGGSGRLAMTDPDDPTTSGVEIIARLPNRREQGLALLSGGERSLTAAALIFALLR
                     VAPPPFCVLDEVDAALDEANVNRFRDLLGELSGQTQFILITHNRGTVQAAHTLYGVSM
                     QPDSSSQVISIKPEEYLSRTAES"
BASE COUNT          900 a         1925 c         1947 g          903 t
ORIGIN      
        1 gtagacgtac tgcgccccca gttgcagcaa tttctgggcc ttgcgcggcg tcagcgcctc
       61 ctccggcgcg ccataggccc ggccgcggcg cgtcttcacc tccacaaaca caagcgtctc
      121 gccgtcgcgg gcgacaaggt cgatctcgcc cacggcgcag cgccagttgc gggccacgat
      181 ggcgtagccc ttggcctcta actggacggc agcgacgccc tcgccccagt tgcccagccg
      241 gcggcgcggc gcgaccggct cagtcatgcc gctcccggcg cgctgtcagg acgttggccg
      301 gccaccagcg cggcgggcca ctgtcggcgt cgtccgacgg ccggacgagc tgcgtcagtt
      361 gctccagcat ggccgaccac ttctccgtcc ggcgcgacca gcggccgtgg gcgtcctcac
      421 gctgcaagtc gggccgctcg gccagcaggg agtcgtatag cgcgacggtc aggtcgatca
      481 tgcgggcgat gtcaaagcgg gcgctggcgg cgcgagcggc ctcgcccatg gcccgtgccc
      541 gcgccggatt ggccgccagg gcagcgatgg ccgccgacaa gccctccacg tcggggacga
      601 tcagaccagt cacgccagac tcgaccgaat cgctgatgcc cggcgactgc acggcgatga
      661 tgggcttacc cgcggccatg gcctcgatga ccgtcagcgg atgcacctcg ctcgtggagg
      721 cagtgacgaa ggcgtcggcc gcggccagcc agtcgccaac ctcatcgaag ggcacgacgc
      781 ccacgaagtg gacgcgctca tccagttggc gctcaaccac caggcggcgc aactcctctt
      841 cttgcggccc cttgccggcg atggccagcc gcagcgcggg catgaccccg gcggccaggg
      901 cgaattgcgc cagcaggccg gccaggttct tctcctcggc cagccggccg gtgtagagca
      961 gcagaaaggc gtcgtcgggc acgccgaagt cggctttggc ccgcgggcga agggggcgca
     1021 agaacgattc gcgctcgatg ccgttctcga tgaccacaat agggttgctg acgccaaagt
     1081 cgatcagcaa ttggcgcaca ctggccgacg gggcgatgac cgtgtcggcc agaccggcaa
     1141 actccggcca cagctggcgc atgatggcgt cggccgccgg ttggggcagc ggcgtgtagg
     1201 ccccggtgta aagatcgtag cgggtgtggt tggtatagac gatggggcac tgagcgtagc
     1261 ggtgggccat ctccacgctc atgagcaagt ggtggcagtg gacgatctcc atggtctgca
     1321 acaggcgctg cgcctcctgg ctgtagccca ggccgataaa gtagccatgt tcccccagcc
     1381 gcatgcccgg cgagcggaca atgcgcgcgc cagcgtcgtc cgcctgctcc tcgcccagcg
     1441 tgaagatggt gacctcgtga ccacgcgctt ccagatgacg tttgtagagt gtcaccatgc
     1501 gcacgacccc gttgatcaca gtgggatcat acgtggccga aaccatgccg atgcgcatcg
     1561 tccctccctt gtctagcccg cctgtcgctg ggtcagtcgg gcgcattctc ctgtagctgc
     1621 aactggtatt gccgcactag ccagtcggtg ttgcggatct tggtggccag ttcggcggcc
     1681 agattgcgca tgagcttgaa gcccagcacg gggtaggtct cgcccaggcg catgagttga
     1741 tcgcggcgca ggcgcagcag cagcgtgtcg tcgcggctga cgcggatggt ggccgagcgc
     1801 acgccctgat cgaccaaagc cacctcgcca acgacctgcc cctgccgcaa ctcgctcagc
     1861 acgaccggct ccagcggcgg ccggccccct tcgcccgcgc ccgtgttgtc gccaacggcg
     1921 ttggggttca ccaggacttc cacgccaccc cggccaatga cgtacatctc gtcggaagcc
     1981 tcgttctcgc tcaggacgat gtagccacgg cggtaagtga ccggcgtgca caaggcggcc
     2041 accaacgtca gttgcgcctc gctaagattg tcgaagattt ccgttgtcgc caggatggtg
     2101 cgaatgctgg gctgcatggg ccacgtctcc tcgccctact gctcattgcc ttgctggccg
     2161 tagatgacgc tcatgccccg cgcccggccc agcagccact cggccacggc aatgacggcc
     2221 atgaaaagca catcgacggc gatggcgatg tagagggcca cagccaggta gccgaaaaag
     2281 gtcgccaccg gcccggccag gttgtcgagc acggcgcggc cccgcgtgac aaaggcgacg
     2341 acgagtatca ggacggccag ccatagcagg aaggccggcc aggcgtggcg gtcggactta
     2401 ctgggcagca tggcgttgcc gatggcaaag agcaggtaaa gccacagggc gaaggccggc
     2461 gtggccagca gctcggtcat ggccgccgcc agggcattga tgtcgccggt ctgcaccgcc
     2521 gcggccaggc tggtgacgcc gaagacgcgg taaccgatga acaggatgac cgccgtgccg
     2581 gcgatgagcg gcgcgccgcc gatgaggctc tcgcgcagtg ggtcgagcgt ccggccgcga
     2641 tagtactcga cgtagcccag ttgcagtgtc ccgtccggct ggcggcgcgg cagcagcgac
     2701 acgccaccgg tgcgcacgcc cagcagcgtc gccgacagcc agtggctggc ctcatgcagc
     2761 aggacgccgg gaaagagaat gatggcgtaa acaaccaggg ccagttccgc ccggccggtc
     2821 agcaacagac tgacgccgtg caggtgggcg tggatccagc gctgcaagaa gaccagcagc
     2881 accagggcca cggccgcggc caggaagggc atccacgggt cgttcatagg cccctggcgc
     2941 ttagaccact gcgccgcgca ccagatggac gaagctcgtc ttcagcgacg ccccaccgac
     3001 cagcgcgccg tcaatgtcgg gctggcgcat gaactcagag atgttctccg ccgtcacgct
     3061 gccgccgtac tggatgcgaa tagcctgggc agtggcctcg ccgaacatct cggtcagcac
     3121 gtcgcggacg ctggcgcgga tgatgtcctc ggcctgctgg gcgctggccg acaggcccgt
     3181 gccgatggcc cagatgggtt cataggcgat gacacactcg gccgcctgcg ccggcgagca
     3241 gccggcaaac gccgcccgca cctggccgct gacgaacgat tgcgtctggc cggcctggtt
     3301 ttgttccagg ctctcgccga cgcagacaat gggcgtcagg ccatgcacca gcgcggcgaa
     3361 aatcttcttg ttaacaccct cgtcggtctc gccgaaatag gcgcggcgct cggagtggcc
     3421 caggatgacg tactggcaaa ggccttgcag catggccggg gagatttcgc cggtgtaggc
     3481 gccgctcgtc tcgtagtaca tattctgcgc gccgacgcgg acgcgcgtcc cggccagggc
     3541 gcgggccacg gcggggaggg cgatgaaggg cgggcagacg gcgatgtcga tgccgttgat
     3601 ggcgttgaga tcggcggcga tctcgcggac gaattctacg gcggcgtcgg ccgtcttgtt
     3661 catcttccag ttgcctgcaa cgaatggggt acgcatgatg tctttatgtt tcctcggttg
     3721 ggcgggtcgg tcagcggtga tggcgtcaga gagcaaccgc ccgttgtttc gctagataag
     3781 ctcattataa gagaggcggg gcgcggcggc aattcgtggc gcgattctcc agaatcgcgt
     3841 tctggatcgc gccgcatcac gctccacgct ggagggaaag agcgattctg gagaatcgcg
     3901 ccacgctacg actcggccgt ccggctcaga tactcttcgg gcttgatgga gatgacctgg
     3961 ctggaactgt cgggctgcat gctgacgccg tagagcgtat gcgccgcctg caccgtgccg
     4021 cggttgtggg tgatgaggat gaattgcgtc tgcccgctca actcgcccag caggtcgcga
     4081 aagcggttga cgttggcctc gtccagagcg gcatcgacct catccagcac gcaaaagggc
     4141 ggcggcgcga cgcgcagcag ggcgaagatg agcgccgcgg cagtcagaga gcgctcacca
     4201 cccgacagca gcgccagccc ttgctcgcgg cggttgggca accgggcgat gatctccacg
     4261 ccgctggtcg ttgggtcgtc ggggtcggtc atggccagcc ggccagagcc gccgccgaac
     4321 agttggcgga aggtctggtc gaagatgccg ttgacctgtt cgaccgtccg ggcaaaggcg
     4381 accgaggtca gctcgtccag ttcggtgatg accgcccgca gccgcgcgtc cgtctcgctc
     4441 aggtcgccca cctgctgggt catgaactcg aagcgttcct cggtgctggc caactcggcc
     4501 ggggcgtcgg ggttgatcgg ccccatgcgc tgcaactggc cgcggtagtg gtggatattc
     4561 tcctcgatgt cggccggcag ttcggccacc acgggcaact cagccaccac gtcgcgcatg
     4621 ggcagcggcg tagccccggt ctgctcagcg tcgtagctga gggccaccag gcccaaatcg
     4681 gcgcggatgc gctcttgtag agcctcaatg tggttctctt gctgcgtctg gcgaatgtgg
     4741 gtctgggtca gctgcgtctc caactcgtgg gcgtggcgct ggcggggcga cagatcggcc
     4801 tgcaagcggg ccagcagggc gcggtcgctc tcggccgagg ccagtagcgg ggccagttcc
     4861 gtctccacct cgaccagtcg ccgctcggca acgatcagat cggcttcgtc ttcgctctcc
     4921 acctcagcca actggccgcg cagcgcctcc acgcggcggc gcagacgttc tagctgccca
     4981 tcggcctggt tcagcgtcgc ccgccggcta tcgacgaccg cctgtcgccc ggccacgatg
     5041 gtctgggccg cggccacact ctggcgcagg ctctgttgct gctgctcggc ctcgcccacg
     5101 ggcaggtcag ccagttgcgc ccgcgccgcg gccagcgccg tctccgtctg ggcaacgcgc
     5161 tccctctgcc gggccagggt ttcctccgtc tgcgcgatgc ggctctgcaa gctctgaatg
     5221 gctgtctggc gctcggcacg gcggcggacg gcgaagtcgc gctgctgctg ggcgcggtca
     5281 agctcccggc gcgcggccgt cagccggcca gcagcctcgg cctccagccg gctcaggcgc
     5341 tgctcctcgg cggcgcggcc atcgacgtcc tcttgcagcc tgtcgatagc tgccgcccgg
     5401 ccggcggcgg ctgcttcggc ttcggccagc gcggcgtgca gcgtctccag cgcggtggcg
     5461 gcctcgcggc ggcgcgcctc ttgggccagc acgccggccc cgccaccgtg gacttccact
     5521 aacccgccgc cgcgaacgac aacgccatcg ggcgcgacgg cggcgtagcc cggtggccag
     5581 tcgcgcgcca ggcgataggc cgtggcggcg tcctccacca gcagcagccg gccgagtagc
     5641 tgcccggcca gcggcgcggc ggcgatgtcg aaggc
//
LOCUS       DAOSMQ010000181         5662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14432_length_5662_cov_4.914036, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000181 DAOSMQ010000000
VERSION     DAOSMQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14432_length_5662_cov_4.914036"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..950)
                     /locus_tag="PK829_07345"
     CDS             complement(21..950)
                     /locus_tag="PK829_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU41067.1"
                     /translation="MMRTLSLLLLLLFTTVIPVAAQDETTELDQMVVELWPDYDRPAM
                     LVLLTGTLPAATTLPATLSIPLPAEADIHAIARFTDEDVLISDVESTAADGRLTLTTA
                     GRTFRVEYYVPYARDGSQTSYRFEWQSGLTIGSMSAVVQQPLAATNFAVVPEPDSSAA
                     DRGDGLNYHQIATRPLAAGEPFTVEVTYSVDAPVLSAPSQSPTAPTATPAAAGNGPLD
                     GLSPWWLLAIPAALALLVGAWYLGRQTGSGRARKPRPARAAKPGGPRNGSAPRGGGNS
                     GANSGAAGFCHHCGQPAQPGDVFCRKCGTRLKQ"
     gene            complement(1025..1324)
                     /locus_tag="PK829_07350"
     CDS             complement(1025..1324)
                     /locus_tag="PK829_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41068.1"
                     /translation="MKQMRRAAIYKLAASAHEMELDVMNGVLRRDDDGRWHIGDYDLL
                     TWLDAHRGEELVVVLGTMADEREVQVRTCRTCGRDYTELECPYCRANRIRLRGHA"
     gene            1445..2047
                     /locus_tag="PK829_07355"
     CDS             1445..2047
                     /locus_tag="PK829_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41069.1"
                     /translation="MHNGSERLAQDIAALSAMAAQMAEYLDSDILFWPLGHSSLPMLT
                     LGGYLLREHRLLALPQLLTLEQRGQVDAAIFQFNQALSNRIVRFETKAQRELESRLRQ
                     WEESVREMEHGTLQRSSNYSTIVETRAMIQAVLDRLDMPPYRPDERSGRLVTLLDTRL
                     RNRWQPGEFVWPAEWQPAYPRDDYWWLYGSPRVAKYSGND"
     gene            2174..3493
                     /locus_tag="PK829_07360"
     CDS             2174..3493
                     /locus_tag="PK829_07360"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41070.1"
                     /translation="MNLRFPPALRYRDYRIFWFGLFLSVIGSQMQLIAVNWHIFELLR
                     GQTLTLNILGRTIDLGAEALGLGTLGLVRVIPIVLFALVGGMLADTRNRRTLLFYAEI
                     AAALMAGTLGVLTLLGRESVPLIYLFTAALSAVAAFENPSRQSLVPHLVPPEHFTNAV
                     SVQTLTWTTAAILGPALAGLLVAYVDTGFVYIINALSFLAVVISLLLMSYRGRAAASS
                     AGLGLGALLDGLRFVFRTPIISSTMLLDFIATFFSSARTMLPIVASDILGVGAVGYGW
                     LATADSVGAVVAGSVLATRGEQLRRQGPILLVCVAVYGAATALFGISTAFLLSFFLLM
                     LVGAGDAISTVIRGTVRQLSTPDELRGRMTSVNMMFFMGGPQLGELEAGLVAAALGVP
                     FAIISGGLATVALTALVAWKFPILRRYDTPTTTLLEAPAAPAAQAGG"
     gene            complement(3509..4279)
                     /gene="cofE"
                     /locus_tag="PK829_07365"
     CDS             complement(3509..4279)
                     /gene="cofE"
                     /locus_tag="PK829_07365"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOU41071.1"
                     /translation="MTTNLLLTALPGIPDVRSGDDVAALLLQALDRAGQTLRDGDVLA
                     VAQKIVSKAEGRQVRLAAVTPSARAEEVAAVVGKDARLVELILRESTAVSRLAPGVLI
                     VRHRLGFTSANAGIDRSNVQTHSGEEEAVLLLPLNPDASAVALREALRAATGAAAGVV
                     ITDSHGRPFRLGTVGVAIGVAGLPALWNRRGEADLYGYRLQHTDVGLADEIAAAAGLL
                     MGQAAEGLPAVLLRGLRLPPTGGRATDLVRPDELDLYR"
     gene            complement(4504..5160)
                     /gene="npdG"
                     /locus_tag="PK829_07370"
     CDS             complement(4504..5160)
                     /gene="npdG"
                     /locus_tag="PK829_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HOU41072.1"
                     /translation="MKIAILGGTGKEGSGLGFRWAAAGHEVIIGSRTEAKGQRAAEEL
                     QAALPEATIRGCDNVTAAADAELVVLSVPYEAQQATLTDVRAVLQGKMLVTVVAPLGQ
                     PKARVMRLPSGRSAAEEAQMQVGSNVTVIAAFQNISASHLRDLDHALDCDVLICGERA
                     ADKEIVAGLCREAGMRGINAGPLANAVVAEGLTAVLLGINVRHKITGAGIRITGLPEE
                     "
     gene            complement(5186..>5662)
                     /locus_tag="PK829_07375"
     CDS             complement(5186..>5662)
                     /locus_tag="PK829_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU41073.1"
                     /translation="VGAAPVESLLRLGVPVCLGNDGMGNAMWQEWKAAYFMHKQAHRD
                     PRRMNGTDVARMAIDNNAALAGVFWPDLPLGRLAVGAAADVIFVDYHPFTPLTAGNLP
                     WHLIFGFESSMVTTTIVGGRILMRDRQLLTLDEAAIAARSRELAVKVWRRFEELSN"
BASE COUNT          873 a         1901 c         1897 g          991 t
ORIGIN      
        1 gggggagagc gtgggagcgg ctactgcttc agccgcgtgc cgcacttgcg gcaaaacacg
       61 tcgccgggct gcgccggctg gccgcagtga tggcagaagc cggccgcgcc gctgttcgcg
      121 ccgctattgc cgccgccgcg gggcgcgctg ccgttgcgcg ggccgccggg cttggccgcc
      181 cgcgccggcc gcggcttgcg cgcccgcccg ctgcccgtct gccgccccag gtaccacgcc
      241 cccaccagca aagccagcgc cgccgggatg gccagcagcc accacgggct gaggccgtcc
      301 agggggccgt tccccgcggc tgccggcgtg gctgtcgggg ccgtcggaga ctgcgacggg
      361 gcagacagca ccggcgcatc gacgctgtaa gtcacttcga ccgtgaacgg ctcgccggcg
      421 gccagagggc gcgtggcgat ctggtgatag ttcaggccgt cgccgcggtc ggcggcgctg
      481 ctgtccggct ccggcacaac ggcgaagttc gtcgctgcca acggctgctg gacgacggcc
      541 gacatgctgc caatggtcag gcccgattgc cactcgaagc gataggaggt ctggctgccg
      601 tcacgcgcgt aggggacgta gtattccacg cggaaggtgc ggccggccgt cgtcagcgtc
      661 aggcgaccat cggcggccgt cgattcgacg tcggagatga gcacgtcctc gtcggtaaaa
      721 cgggcgatgg cgtggatgtc ggcctcggcc ggcagaggga tgctgagcgt cgccggcagc
      781 gtcgtcgccg ccggcagcgt gcccgtcagc aataccagca tggccgggcg atcatagtcg
      841 ggccacaatt cgacaaccat ctggtcgagt tccgtggtct cgtcctgcgc ggccacgggg
      901 atgacggtcg tgaacaaaag aagcaaaagc aagctgaggg tacgcatcat agcattattc
      961 gcaagggtta ctgagtggtg ggatagtcga tcaggggccg gggcaaggcg cggcggcggt
     1021 cgtcttaggc atggccgcgc aggcggatgc ggttagcgcg gcagtagggg cattccagct
     1081 cggtgtagtc gcggccgcac gtccggcagg tgcgcacctg cacctcgcgc tcgtcggcca
     1141 ttgtgccgag gacgacgacc agttcctcac cgcggtgggc gtctagccag gtgagcaggt
     1201 catagtcgcc gatgtgccag cggccatcgt cgtcgcggcg caggacgccg ttcataacgt
     1261 ccagctccat ttcgtgggcc gaggcggcca gcttatagat ggcggcgcgt cgcatctgtt
     1321 tcattacgtt cctcactcgc gtcaacggcc gccggggctg gtttagcgcc cggcaagaag
     1381 gtttgacgct gttcaggctg gggtctatta tatccaaaga gcgaattctt caaccggagg
     1441 tctcatgcac aacgggagcg aacgactggc tcaggacatc gccgccctga gcgcgatggc
     1501 cgcgcagatg gccgaatatc tggatagtga catcctcttc tggcccctcg gccatagcag
     1561 cttgccgatg ctgaccctcg gcggctatct gctgcgcgag catcggctac tggctttgcc
     1621 gcaactgctg acgctggagc agcgcggcca ggtagacgcg gccatcttcc agttcaacca
     1681 ggccctcagc aatcgcatcg tccgtttcga gactaaggcc cagcgcgagt tggagtcccg
     1741 tctgcgccag tgggaagagt cggtccggga aatggaacac ggcacgctcc agcgcagtag
     1801 caattacagc accatcgtcg agacgcgggc catgatccag gcggtgctcg accggctgga
     1861 catgccaccc taccggccag atgagcggtc tggccgcctc gtaaccctgc tggacacgcg
     1921 tctgcgaaat cgttggcagc cgggcgagtt cgtctggccg gccgaatggc agcccgccta
     1981 cccgcgcgac gattactggt ggctctatgg ctccccgcgc gtggccaagt atagcggcaa
     2041 cgactagacg ggccggccgc cggcgacaga ccgcagaccg cagacgacag acgacagacc
     2101 acctcttctg gccgtggtcc gtcgtccgtc gtcgctctaa agacgactaa ctccaggcac
     2161 ctttcagacg tccatgaacc tacgcttccc tcctgccctg cgatatcgcg actatcgcat
     2221 cttctggttt ggcctcttct tgtccgtcat cggctcgcaa atgcagctca tcgccgtcaa
     2281 ctggcacatc tttgagctgc tacgcgggca aaccttgacg ctgaacatcc tcggtcgcac
     2341 catcgacctc ggcgcggagg cgctggggct gggcacgctc ggcctggtgc gcgtcatccc
     2401 cattgtgctg ttcgccctgg tcggcggcat gttggccgac acgcgcaacc gccgcacgct
     2461 gttgttctac gccgagatcg ccgcggcgct catggccggc accctgggtg tgctgacgct
     2521 gctggggcgc gagtcggtgc cgcttatcta tctattcacg gcggcgctgt cggccgtggc
     2581 cgccttcgag aacccctcgc gccaatcgct ggtgccccac cttgtgccgc cggagcactt
     2641 caccaacgcc gtcagcgtgc agacactgac ctggacgacg gcggccatcc tcggcccggc
     2701 gctggccggt ctgttggtgg cctacgtcga taccggcttc gtctacatca tcaacgccct
     2761 gtcgtttctg gctgtggtca tctcgttgct gctgatgagc tatcgtggcc gggcagcggc
     2821 gtcgtcggcc gggctggggt tgggcgcgct gctggatggg ttgcgcttcg tcttccgcac
     2881 gccgatcatc tccagcacca tgctactcga cttcatcgcc acgttcttta gctccgcgcg
     2941 gacgatgctg cccatcgtcg ccagcgacat cctgggcgtg ggcgcggtcg gctacggctg
     3001 gttggccacg gctgactcgg ttggcgcggt cgtcgccggc agcgtcctgg ccacgcgcgg
     3061 cgagcaactg cgccgccagg ggccaatcct gctggtctgc gtggccgtct atggcgcggc
     3121 cacggccctc ttcggcatat ccaccgcctt cctgctgtcg ttcttcctgc tcatgctcgt
     3181 cggcgcgggc gatgccatct cgaccgtcat ccgcggcact gtgcggcagc tctccacgcc
     3241 cgacgagttg cgcggccgca tgaccagcgt caacatgatg ttcttcatgg gtggcccgca
     3301 attgggcgag ctagaggcgg ggctggtggc ggcggccctg ggcgtgccct tcgccatcat
     3361 cagcggtggc ctggcgacag tggccctgac ggcgctggtg gcctggaagt tccccatcct
     3421 gcggcgctat gacacgccga caacgaccct gctggaagcg ccggccgcgc cggcggctca
     3481 ggccggtggg taagcgccca cgcaccgctc agcgatagag gtctaattcg tccgggcgga
     3541 cgaggtcagt cgcccgcccc ccggtcggcg gcagccgcag cccgcgcagc agcactgccg
     3601 gcagcccctc agccgcctgg cccatgagca gcccggcggc ggcggcgatc tcgtcagcca
     3661 ggcccacgtc ggtatgctgc aagcggtagc catacaggtc ggcctcgccg cgacggttcc
     3721 acagcgcggg cagcccggcc acaccgatgg ccacgccgac cgtgcccagc cggaaggggc
     3781 ggccgtggct gtcggtgatg accacgccgg cagccgcgcc cgtggccgcg cgcagcgcct
     3841 cgcggagagc gacggccgag gcgtcgggat tgagcggcaa cagcagcaca gcctcttctt
     3901 cgccgctgtg ggtctgcacg ttggagcggt cgatgccagc gttggcgctg gtgaagccca
     3961 ggcggtggcg gacaatgagc acgccggggg cgaggcgaga cacggccgtg ctctcgcgca
     4021 aaatcaactc caccagccgc gcgtccttgc cgacaacggc agccacttct tcggcccgcg
     4081 ctgagggcgt cacggcggcc agccgcacct gccgcccctc agccttggag acgatcttct
     4141 gggccacggc cagcacgtcg ccgtcgcgca gcgtctggcc ggcgcggtcg agcgcctgaa
     4201 gcagcagcgc ggccacgtcg tcacctgaac gtacgtcggg aatgccgggg agggcggtga
     4261 ggaggaggtt agtggtcagt ggggcagtat ccagtggggt agtatccagt gggacagtat
     4321 ccagtggggc agtatccagt gggacagtat ccagtggggc agtatccagt gggccagtat
     4381 ccagtagatc agtaggtcag tggtcagtag gtcagatcag tttgtgtact gacctactga
     4441 atactgtctt actggatact gtcttactgg atactgtctt actggatact gacccactga
     4501 cttctactct tccggcaagc cggtgatgcg aataccggcg ccggtgatct tgtggcggac
     4561 gttgatgccc agcagcacgg ccgtcagccc ctcggcgacg acggcgttgg ccagcggccc
     4621 ggcgttgatg ccgcgcatgc ccgcctcacg gcacagcccg gcgacgatct ccttgtcggc
     4681 cgctcgctcg ccgcaaatga gtacgtcgca gtcgagggcg tggtccaggt cgcgcaggtg
     4741 gctggcagag atgttttgga aggcggcgat gacggtcaca ttgctaccga cctgcatctg
     4801 cgcttcttcg gccgctgacc ggccgctggg cagccgcatc acgcgggcct tgggctggcc
     4861 caacggggcg acgacggtca ccagcatctt gccttgcagc acggcgcgca cgtcggtcag
     4921 cgtcgcctgc tgcgcctcat agggcaccga caggacgact agctcggcgt cggcggcggc
     4981 ggtgacgtta tcgcagccgc ggatggtcgc ctccggcaag gctgcctgta actcctcggc
     5041 ggcgcgctgg ccctttgcct ccgtgcgcga gccgatgatg acctcatgcc cggccgcggc
     5101 ccaacgaaag cccaatccgg aaccctcttt gccggtcccg cccagaatcg cgatcttcat
     5161 ggtctattcc cctttgccgt tttcctcagt tgctcaattc ctcgaaccgc cgccacacct
     5221 tgaccgccag ttcccgactg cgggcagcga tggccgcctc gtccagcgtc agcagttgcc
     5281 ggtcgcgcat taatatccgg ccgccgacga tggtcgtcgt caccatgctg ctttcgaagc
     5341 cgaagatgag atgccagggc aggttgccgg cggtcagggg cgtgaaggga tggtagtcaa
     5401 cgaagatcac atcggccgcc gcgccgaccg ccaaccggcc gaggggcaaa tctggccaga
     5461 agacaccggc cagagcggcg ttgttgtcaa tggccatgcg ggccacgtct gtgccgttca
     5521 tgcgccgcgg gtcgcggtgg gcctgcttgt gcatgaaata ggccgccttc cactcttgcc
     5581 acatggcgtt acccatgccg tcgttgccca ggcagaccgg cacgcccagc cgtagcaggc
     5641 tctccaccgg ggccgcgccg ac
//
LOCUS       DAOSMQ010000182         5659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14436_length_5659_cov_5.152926, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000182 DAOSMQ010000000
VERSION     DAOSMQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14436_length_5659_cov_5.152926"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK829_07380"
     CDS             <1..201
                     /locus_tag="PK829_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41074.1"
                     /translation="LQAHLLAADGTQQVADGLGYAADQWQPGDWFAQRHVFAAPGEAL
                     ETGLYNYITLERAGPVARLGQP"
     gene            198..2510
                     /locus_tag="PK829_07385"
     CDS             198..2510
                     /locus_tag="PK829_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41075.1"
                     /translation="MNQALTPRATGRLLALILGLYALLAVAYSVASPPFETPDENLHY
                     FTADFIARNGRLPTTSDPGGMGQEAAQPPLYYAAAALLIRALDRDGQPAQLWPNPRVD
                     VSSVAGNLWISPLAPPNPRGESGARPAINANMFVRAPEEQWPWRGHVLAAHAARLLSA
                     ALGLGTLLCIYAAGRAVWPGAPGRALLATALVAFLPQFAFIHAAVSNDAAITLFCSAA
                     LWQLLRLLNTRPNPTSNGVTLAPPLARSPAPLLFLGLTIGLALLSKAAGLLLLATGAG
                     VVGLLALASAWPEGAARAAARAAAAVAWVVAPALLLGGWLLWRNWTLYGDPTAANQFV
                     AIAGGYRPYSLLQVAQDMDRVWLSFFALFGWMSVQPPAWIHVAWNLIVLLAALGAGWG
                     VVRGRRWAGARRFVARHASFLVLLGWFLLVSAGWLQFILRTSADQGRLFFPALVPLAL
                     AAAYGLSHWPRPWTQLAAVGVALATSVYCVVVVLPETFARPAPVSAVPAAVAPRDTRF
                     DEGLTLLGARVETATARPGDWVWATLYWQADATGRAASPLVYLELFGRDFARIGNVVA
                     FPGRGGYPAAFWPAGAIIGDRLAAQVRLPDATAVPVEAQLAVKLSEDADSVAIGAVKV
                     VPARWPTPVEPLATLGTGIELARAELAADEAAPGESIAVRLRWQVADPPPGAADLHLF
                     VHLGDPTQAPLAQSDGPVMGGQYPPRLWATGEVFDETAVLALPAGLPPGDYPVQIGLY
                     DFATGARLPVTAGGQHVPSDTVPVGVLRVR"
     gene            complement(2566..2862)
                     /locus_tag="PK829_07390"
     CDS             complement(2566..2862)
                     /locus_tag="PK829_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005442477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOU41076.1"
                     /translation="MLKKKYVKSKDEYEVTFELPSEASEAMLVCEANGWEPVAMKKSK
                     GTFAAKLRVPANGRYQYRFLLNNNEWVNDDAADAYVPNEHGSINSVVETYSNGH"
     gene            complement(3099..5345)
                     /locus_tag="PK829_07395"
     CDS             complement(3099..5345)
                     /locus_tag="PK829_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOU41077.1"
                     /translation="MIERDTITADAEQLFELINTYLSETDRLQVQKAYDFARREHGDQ
                     RRKTGELFITHPVTVAGYLATYRLDATAVVAALLHDVAEDIPVPIEDIATEFGREVAA
                     LVDGVTKLKEVTRGVAQGRKLSPEELQQATLQKLFAAMTNDVRTVIIKLFDRLHNMRT
                     IHGMSLAKQRAKAVETLAVYAPLAYRLGMWQVKGELEARSLAVLDPHAYETIRAELER
                     LRRHQEPRFEALRVQIEATLRAAGLPAREINLSPENVYTVYQDLVKHGMGYRDVDRNL
                     RLTVLMDDPIDCYTALGHLHTMWRAVPHRIDDYISVQRENLYQSLHTTVVDDNGQPVK
                     IRVRTIDMERRAQIGVLARWYYAGTALSVDGLAEHLGSFFANVKDSISVEPHNVELSV
                     QSALEDVLGNQIRVYTPTGEPKDLAEGSTPIDFAYKIHTELGNRCHAVYVNDLLYPLN
                     RPLKNGDQVRIVKNMRAQPQRAWLDEDLGYITTTYARMHARRWFRRLPEGQAIGQGQL
                     LLQHELEMLGLPDLPPSSIALLLGYEDATELYYNLGRAELLPTTLSTRIMSDQWSKGR
                     CLPIDTVVTAADGSTYLITNADGHRLHLCATCDPRPRDGIVGYLRKDGAVTVHREGCR
                     TLNAMRARAEGVHHLLKLGWGNTQQREARSVLLYVDVYDRPRLLNEITHLMQEKESNI
                     AHICTTRHDRPGELTIEMILEVVSPRQLVRILHQTEALVNVKDVRCAAVPAEDNDDAT
                     PSSLYRPE"
     gene            complement(5527..>5659)
                     /locus_tag="PK829_07400"
     CDS             complement(5527..>5659)
                     /locus_tag="PK829_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOU41078.1"
                     /translation="EETHYLRLYINYLRKKIEEDPANPRYILTERGVGYRFIDFKKE"
BASE COUNT          816 a         1855 c         1963 g         1025 t
ORIGIN      
        1 ttgcaggctc atctgctggc cgccgatggg acgcaacagg tggccgacgg gctgggctat
       61 gcggccgacc agtggcaacc gggcgactgg ttcgcgcagc gccacgtctt tgccgcgccg
      121 ggggaggcgt tggagacagg gctatacaac tacatcactc tggagcgcgc cgggccggtg
      181 gcgcgcctgg gccagccatg aaccaagccc tgacccctcg cgccaccggc cgcctgctgg
      241 ccctcatcct gggcctctac gccctgctgg ccgtggccta cagcgtcgcc tcgccgccct
      301 tcgagacgcc cgacgagaac ctccactact tcaccgccga cttcatcgcc cgcaacggcc
      361 gcctgccgac gacgagcgac cccggcggca tggggcagga ggccgcccag ccgccgctgt
      421 actacgccgc cgccgcgctg ctcatccgcg ccctcgaccg cgacggccaa ccggcgcaac
      481 tgtggcccaa cccgcgcgtc gacgtgtcca gcgtcgccgg caacctgtgg attagcccgc
      541 tggccccgcc gaacccgcgc ggcgagagcg gcgcgcgccc ggccatcaac gccaatatgt
      601 tcgtccgcgc gccggaggag caatggccct ggcgcggcca cgtcctggcc gcccacgccg
      661 cgcggctgct gtcggcggcg ctggggttgg gcacgctgct gtgcatctac gccgccgggc
      721 gcgccgtctg gccgggagcg ccgggccggg cgctgctggc cacggccctg gtcgccttcc
      781 tgccgcagtt cgccttcatc cacgccgccg tcagcaatga cgccgccatc accctgttct
      841 gctcggctgc cctctggcaa ctcctgcgcc tccttaacac ccgccccaac cccacaagca
      901 acggcgtcac tcttgctccc ccgctcgccc gctcccccgc ccccctgctc ttcctgggcc
      961 tcaccatcgg cctggccctg ctctccaaag cggccggcct gctgctgctg gccactggcg
     1021 cgggcgtggt cgggctgctg gcgctggcgt cggcgtggcc ggagggcgcg gcgcgagcgg
     1081 ccgcgcgggc ggccgcggcc gtggcctggg tggtcgcgcc ggcgctgctg ctgggcggct
     1141 ggctgctgtg gcgcaactgg acgctctatg gcgacccgac cgcggccaac cagttcgtgg
     1201 ccatcgccgg cggctaccgg ccctactcgc tgctgcaagt ggcccaagac atggaccgcg
     1261 tctggctgtc gtttttcgcc cttttcggct ggatgagcgt ccagccgccg gcgtggattc
     1321 acgtggcctg gaacctcatc gtcttgctgg ccgcgctggg ggcgggctgg ggcgtcgtcc
     1381 gcggccggcg ctgggccggg gcgcggcgct tcgtggcccg gcacgcgtcg ttcctcgttt
     1441 tgctgggctg gtttttgctg gtgtcggccg gctggctgca attcatcctg cgcacctcgg
     1501 ccgaccaggg gcggctgttc ttcccggcgc tcgtgcccct ggcgctggcc gcggcctatg
     1561 gcctgagcca ctggccgcgg ccgtggacgc aactggccgc ggtgggggtg gcgttggcca
     1621 cgtcggtcta ctgcgtcgtc gtcgtcttgc cagagacctt cgcccggccg gcccctgtca
     1681 gcgccgtgcc ggctgcggtt gccccgcggg acacccgctt cgacgagggg ctgacgctgc
     1741 tgggagcgcg ggtggagacg gctacggcgc ggccgggcga ctgggtctgg gcgacgctct
     1801 actggcaggc cgacgcaacg ggccgggccg cgtcgcccct ggtctacctg gagctattcg
     1861 gccgcgactt cgcccgcatc ggcaacgtcg tcgccttccc cggccgcggc ggctatccgg
     1921 cggcgttctg gcccgccggg gccatcattg gcgaccggct ggcggcgcag gtgcggctgc
     1981 ccgacgccac ggctgtgccc gtcgaggcgc aactggccgt caagctgagc gaggacgccg
     2041 acagcgtcgc catcggcgcg gttaaagtcg tgccggcgcg ctggccaacg ccggtggagc
     2101 cgttggcgac gctgggcacg ggcatcgaac tggcgcgggc cgagctggcc gcggacgagg
     2161 ccgcgccggg cgagagcatc gccgtgcgcc tgcgttggca ggtggccgac ccgccgcccg
     2221 gcgcggccga cctgcacctt ttcgtccacc tgggcgaccc gacccaggcc cccctggccc
     2281 agagcgacgg gccggtcatg ggcgggcaat acccgccgcg gttgtgggcc acgggcgaag
     2341 tgttcgacga gacggcggtg ctggcgctgc cggccggcct gccgccgggc gactacccgg
     2401 tgcagatcgg cctgtacgac ttcgccaccg gcgcgcgcct gcccgtgacc gccggcggcc
     2461 agcacgtgcc gtcggacacc gtgcccgtgg gcgtcctgcg cgtgcgctag aaaacaaaag
     2521 cggcctgccg aagcagaccg ccgccatctt agaatgggac agggcctagt ggccgttgct
     2581 gtaggtctcg acaacgctgt tgatgctgcc gtgctcgttg ggaacgtagg catcggcggc
     2641 atcgtcgttg acccactcgt tgttgttgag caggaagcga tactgatagc ggccgttggc
     2701 cggcacacgc agcttggccg caaacgtgcc cttgctcttc ttcatggcca ccggctccca
     2761 gccgttggct tcgcagacca gcatggcctc actggcctcg cttggcagct cgaaagtgac
     2821 ttcgtactca tccttcgact taacatattt ctttttcagc atggtagatt ctccttctat
     2881 taggtctgtc ggcgcgctat tgcattttgg taaggcaatg ggcttaggtg acaaatgtca
     2941 tttggaaacc gcgtctcatt atgaatgacg gcaagggcca ttgtcaatcg acagagacgc
     3001 agccggtaag cggcggggtc gttttcttag ctcgtactca gctaaagggg cgggaacggc
     3061 tcagagacca gcgtggtgga gagtgcgccg gccgccgctc attcagggcg atagagcgac
     3121 gagggcgtgg cgtcgtcgtt gtcctcggcg ggcacggcgg cgcaacgcac gtccttgacg
     3181 ttgaccagcg cctccgtctg gtgcaggatg cgcacgagtt gccgcgggct gacgacttcc
     3241 aggatcatct cgatggtcag ctcgcccggc cggtcgtggc gggtggtgca gatgtgggcg
     3301 atgttgcttt ctttttcctg catgaggtgg gtgatctcgt tcagcaacct gggacggtca
     3361 tagacatcga catagagcaa cacactgcgc gcctcgcgct gttgcgtgtt gccccagccc
     3421 agcttcagca aatggtggac gccctcggcg cgggcacgca tggcgttcag cgtccggcag
     3481 ccctcacggt ggacagtgac cgcgccgtct ttgcgcaggt agccaacgat gccgtcgcgc
     3541 gggcgcgggt cgcaggtggc gcagagatgt aggcggtggc cgtcggcgtt ggtgatcagg
     3601 taggttgagc cgtcggcggc agtgacgacg gtgtcaatgg gcagacagcg ccctttcgac
     3661 cactggtcgg acatgatgcg tgtcgatagc gtcgttggca gcagttcggc gcggccgagg
     3721 ttgtagtaca actccgtggc gtcctcatag cccagaagga gggcgatgct gctggggggc
     3781 agatcgggca gacccagcat ctccagttcg tgttgcaaca acagctgccc ctggccaatg
     3841 gcctgcccct ccggcaggcg gcggaaccag cgccgggcat gcatgcgggc gtaggtggtc
     3901 gtgatgtagc ccagatcttc gtccagccag gcccgttgcg gctgggcgcg catgttcttg
     3961 acgatacgca cctggtcacc gttcttcagc ggccggttca gcgggtagag caagtcgttg
     4021 acatagacag catgacagcg gttgcccagt tcagtgtgaa tcttgtaggc gaagtcgatg
     4081 ggcgtgctac cctcggccag gtctttcggc tcgcccgtgg gcgtgtagac acgtatctgg
     4141 ttgcccagga catcttccag ggcgctctgg acgctcagct caacgttgtg cggctcaacg
     4201 ctgatgctgt ccttaacgtt ggcgaagaag ctgcccaggt gctcggccag gccgtctacc
     4261 gagagggcgg tgccggcata gtaccagcgg gccagcacgc cgatctgcgc tcgacgctcc
     4321 atgtcgatgg tgcgcacgcg gattttgact ggctgaccgt tgtcgtcgac gacagtggtg
     4381 tgtagcgatt ggtagagatt ctcgcgctgg acagagatgt agtcgtcaat gcggtggggc
     4441 acggcccgcc acatggtgtg cagatgcccc agggcggtgt agcagtcaat cgggtcgtcc
     4501 atgagcacgg tcaggcgcag attgcggtcc acgtcgcggt agcccatgcc gtgcttgacc
     4561 aggtcctgat agacggtgta gacgttctcc ggcgacaggt tgatctcgcg cgcgggcagg
     4621 ccggcggcgc gcagcgtcgc ctcgatctgg acgcgcagag cctcgaagcg cggctcctgg
     4681 tggcggcgca gtcgttccaa ctcggcgcgg atggtctcgt aggcgtgtgg atccagcacg
     4741 gccagcgaac gggcctccag ttcaccctta acctgccaca tgcccagccg gtaggccagc
     4801 ggggcataga cggccagcgt ctcgacggcc ttggcccgct gcttggcgag ggacatgccg
     4861 tggatggtgc gcatgttgtg caggcggtcg aagagcttaa tgatgacggt gcgcacgtcg
     4921 ttggtcatgg cggcgaagag cttttgcagc gtagcctgtt gcaactcttc gggcgatagc
     4981 ttgcgcccct gggcgacgcc gcgggtgacc tccttcaact tggtgacgcc atcgaccaac
     5041 gcggccacct cgcgcccaaa ctcggtggcg atgtcctcaa tgggcacagg gatgtcctcg
     5101 gccacgtcgt ggagcagggc ggcaacgacg gccgtggcgt cgagccggta ggtggccagg
     5161 tagccggcca cggtgacggg gtgggtaata aatagctcgc cggtcttacg gcgctgatcg
     5221 ccatgttccc gacgagcaaa gtcatacgct ttctgtacct gaaggcggtc agtctcagaa
     5281 aggtaagtat tgataagttc gaataactgc tcggcatcgg ccgtgatggt gtcgcgctcg
     5341 atcatagcga tgttcgcgcc gactgccccg gtactgctcg gtccggtcaa gcggcaaaca
     5401 gcggcccgtc cagcggccgc attggccacc gggtcaaggg tccgctcccg attccgccat
     5461 tataaccccc aatgggagga aaaaacaggg cagaacagga ggaaagtcct gttggggcgg
     5521 gcgggcctat tcctttttga aatcgatgaa acggtagccg acgccgcgct cggtcaggat
     5581 gtagcgcggg ttggccgggt cttcttcgat cttcttgcgc aggtagttga tgtagaggcg
     5641 gagatagtgg gtttcttcg
//
LOCUS       DAOSMQ010000183         5659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14437_length_5659_cov_4.949143, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000183 DAOSMQ010000000
VERSION     DAOSMQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14437_length_5659_cov_4.949143"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            543..1313
                     /locus_tag="PK829_07405"
     CDS             543..1313
                     /locus_tag="PK829_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41079.1"
                     /translation="MKPKLVVLVVVLLLLAMVPALAMAKSEAKPSKAIHFTLDRSQPI
                     QPQIQAALGANAPAPKPGAPAAAAPRPTSVALGIRPNAVQGLVNEGFEGLWPSGNWIV
                     WDFSGLDLCWGTTDYVHKRGHYSAWPAAGCANGYDPSTYPYYVDNMETWMDYPMDLSN
                     ATNGYVRFQFRNDSEFFYDYFVWCASHDGMNLDCNAHTGSTNGTWRLVNINLRNFYGE
                     NYMGDQDFLFTWGFISDSSFSDFGGAYVDAIRIRATGP"
     gene            1420..2196
                     /locus_tag="PK829_07410"
     CDS             1420..2196
                     /locus_tag="PK829_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41080.1"
                     /translation="MKPKLIVFVVALLIVAMVPALAMAKGQAKPEQMVKLVLDMRQPL
                     GPQLDAQLGVTSRGPRAVNAPAAMATRGVGGIRPNAVYALLNEGFEMPDWPGGAPGWL
                     FVEAGTSTVGWDATDIVRKRGQQSLYSAGYNNDPQVNPFYENDMESLAFYEMDLQGAR
                     RVQVRFQYMSDTEFNFDYFLWCSTTDGFIFNCYGQTGSTNGKWRLVQLDSRTDPTLQA
                     LLDQPFAYYGFVFLSDGSVVDRGTFVDAMRIRAWGPSPIQ"
     gene            2528..3796
                     /locus_tag="PK829_07415"
     CDS             2528..3796
                     /locus_tag="PK829_07415"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOU41081.1"
                     /translation="MQEVVIIDGVRTAIGRHAGALSDVRPDDLAALVIREIVARTGID
                     PHEVEEVYFGCANQAGEDNRNVARMGALLAGLPHSVGGVTMNRLCASGLNAANQAARA
                     IAAGEGEVYIAGGVESMTRAPYSLPKNSRAFGPSGNVTAYDTTLGWRYPNPKMEALFP
                     LEAMGETAENIYQMSCDGGIAGGVITREEQDAFALESQRRALEAINAGRFREEIVPVV
                     FPQRKGDPIVFDTDEHPRIKKTADGYALDTSAETLARLKPAFRAGGTVTAGNASGLND
                     GAAALLLMSAGRAAALGLSPLARWVGSAAAGVDPRTMGLGPVPATRKVLARAGLTVDD
                     IDLIELNEAFAVQSLAVMRELGLRPEITNVNGGAIALGHPLGCSGARILTTLLHEMKR
                     RRAAGEPMRYGLATLCVGVGQGEATLVELL"
     gene            complement(3793..>5659)
                     /locus_tag="PK829_07420"
     CDS             complement(3793..>5659)
                     /locus_tag="PK829_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU41082.1"
                     /translation="VLLNWLAALFFGLAVARTLEHTTLATLRDEPSSRGHRLALGGLA
                     AAVALAVMAAAFGVNGQQLILVVLLPGLGWAAARLGLRPGGWLAAALLVGLATAAPLL
                     FFDPDELNLALNLGYRDVGYYAFLATIVGMAAAWALSLAALLLGGRAIGRRAWAASAI
                     LSVVGLALVYALTGQTGWHGERLFVILRDQANIGSAVVINDPGERRAVVYDLLTHHAA
                     ATQADLRQTLDRFGVPYTPYYLVNSLEVQGGPLLRWWLSRRPEVERVLVSPVVRPLPV
                     APALPEANPDVTPPSLLWSQLLIRVPQVWNRLGVTGEGIVVGQNDSGADVTHLELHDN
                     YRGNTPGGPTGDDYNWLDPWNGTTSPTDFGGHGTHTLGTAVGRNVGVAPDATWIACVN
                     LARNLGNPPRYLDCLQFLLAPFPQGGNPFEDGRPDLGANVLNNSWGCPPQEGCEVNTL
                     LPAARALRAAGVFVVVSAGNNGPTCGSIDGPLALYDEVFTVGAVDEARRVAEFSSRGP
                     VTADGSGRIKPDIAAPGVEVTSAVPGNNYVAYDGTSMAGPHVVGVVALLWSANPALIG
                     DIDRTEQILTETAINIAPDAFDGPTCGVVRKQPNSFVGYGIVNALAAVERALAER"
BASE COUNT          940 a         1873 c         1883 g          963 t
ORIGIN      
        1 ggcacggccg tggcctaccg gctgatccgc gagggcatcg ccttcctcga tggcgacgtg
       61 cccctttcgc cccacatcga agtggcgcgc cggctggtgg cggatggtac gatcaagcgc
      121 gctgtcgagg cgcgcctgga catggcttag ggcgcgatgt aggtaatttg tcgcacaagg
      181 gacttaatac ctgctttgtc tcgttatttc gtgcctgatt catcttggga agcaattgac
      241 atcgttaaat tctgccacta tactttcatc tacactgtag ctaagtcaac cgactgcgct
      301 cctctctaaa gtccttccct gccaggcgcg gcggtttctt agtccgtcca cagccacagt
      361 gttcgtgccc tgaccctatt tgcgcgcctg taaaaatgcc tactattgac caggaggact
      421 ctacatgcct aaacggggct tgcccaacgc gttctgaaag cgcttcccac tatgatggtt
      481 taagcacagc gggtggtttt aatacgtgag ccaggcaggc tccaatgctt tgggaggaaa
      541 ccatgaaacc gaaattggta gtgcttgttg tcgtgttgct tttgctggca atggttccgg
      601 cgctggcgat ggccaagagc gaggccaagc cgagtaaggc gatccacttt accctcgatc
      661 gcagccagcc catccagccg cagatccagg cagcgctggg cgcgaacgcc ccggccccca
      721 agccgggcgc gccggccgcg gccgcccccc gcccgacctc cgtggcgctg ggcatccggc
      781 cgaacgcggt tcagggcctg gtgaacgagg gctttgaggg gctgtggcca tctggtaact
      841 ggattgtgtg ggacttctca ggcttggact tgtgctgggg gacgaccgat tacgtccata
      901 agcgcggcca ttacagcgcc tggccggcgg ccggctgcgc caatggctat gaccccagta
      961 cctacccgta ctacgtcgat aatatggaga cgtggatgga ttacccgatg gatctgtcca
     1021 acgcgaccaa cggctacgtc cgcttccagt tccgcaacga ctccgaattc ttctacgact
     1081 acttcgtctg gtgcgcgtcg cacgacggta tgaacttgga ctgcaacgcc cacaccggct
     1141 cgacgaacgg cacgtggcgc ctggtgaaca tcaacctgcg gaacttctac ggcgagaatt
     1201 acatgggcga ccaggacttc ttgtttacct ggggcttcat ctctgactcc agcttctccg
     1261 acttcggcgg cgcctacgtc gatgcgatcc gcattcgcgc gacggggccg tagttgtcgg
     1321 ctcgttcatt accaggccga cgtgcccagg ttgtaatcag tgacaagcgt gcatccgcgg
     1381 cagtagagtc gttctgactc acaagtttag gaggaaacaa tgaaaccgaa gttgattgtt
     1441 tttgtggtgg cgttgttgat cgtggcgatg gtgccggcgc tggcgatggc caagggccag
     1501 gccaagccgg agcagatggt gaagctcgtt cttgatatgc ggcagcccct cggcccgcag
     1561 ctcgacgcgc agctgggtgt cacgtcgcgc ggacctcggg cggtgaacgc cccggcggcg
     1621 atggcgaccc gcggtgtggg cggcattcgg ccgaacgccg tctacgcgct gctcaatgag
     1681 ggcttcgaga tgccggactg gccgggcggg gcgcctggct ggctatttgt tgaggctggt
     1741 accagcactg tcggttggga cgcgaccgat atcgtgcgga agcgtggcca gcagagtctc
     1801 tattcggcgg gatacaacaa cgacccccaa gtcaatccct tttatgagaa tgacatggag
     1861 tcgttggcgt tctacgagat ggacctgcag ggcgcgcgcc gggttcaggt tcgcttccag
     1921 tacatgagcg acactgagtt caactttgac tacttcctct ggtgctcaac gactgacggc
     1981 ttcatcttca attgctacgg gcagaccggc tcgaccaatg gcaagtggcg tctggttcag
     2041 ctcgactcgc gtaccgatcc gacccttcag gccctgctcg accagccgtt cgcgtactac
     2101 gggttcgtct tcctcagcga cggcagcgtt gtcgatcgcg gcacgttcgt ggacgcaatg
     2161 cgcatccgcg cctgggggcc ttcgcccatc cagtagcctt ccggacggcg ctgcggcgct
     2221 tcgtctaggc gtctaaatac tagcccccga ccgtcacgcg gtcgggggct agtttcatgc
     2281 cgtggcgcga ttctcccgaa tcgcctttcg tggcgcgatt ctcccgaatc gcctttcgtg
     2341 gcgcgattct cccgaatcgc cttttgtggc gcgattctcc cgaatcgcct tttgtggcgc
     2401 gtcacgtaaa aacgcgattc ggagaatcgc gccacgtgga cgcagggggc tggcggggta
     2461 caattggccc cattcgccgc cgccggagtt gtagccccca tgtcgccgcg gcgcaaagag
     2521 gcccatcatg caagaagtag tcatcatcga cggcgtgcgc acggccatcg gccgccacgc
     2581 cggcgctctg tccgacgtgc gccccgacga cctggccgcg ctcgtcatcc gcgagattgt
     2641 cgcccgtacg ggcattgacc cccacgaagt ggaagaggtc tatttcggct gcgccaacca
     2701 ggccggcgag gacaaccgca acgtcgcccg catgggcgcg ctgctggccg ggctgcccca
     2761 cagcgtcggc ggcgtgacca tgaaccgcct gtgcgccagc ggcctgaacg ccgccaacca
     2821 ggccgcgcgg gccatcgctg ccggcgaggg cgaggtctac atcgccggcg gcgtcgaatc
     2881 catgacccgc gccccctact cgctgccgaa aaatagccgc gccttcgggc cgtcgggcaa
     2941 cgtcaccgcc tatgacacga cgctcggctg gcgctacccc aacccgaaga tggaggctct
     3001 ctttcctctg gaagcgatgg gcgagacggc cgagaacatc taccagatga gttgcgacgg
     3061 cggcatcgcc ggcggcgtca tcacgcgcga ggagcaagat gccttcgccc tggagagcca
     3121 gcgccgcgcc ctggaggcca tcaacgccgg ccgcttccgc gaggagatcg tccccgtcgt
     3181 cttcccgcag cgcaagggcg accccatcgt cttcgacacc gacgagcatc cgcgcatcaa
     3241 gaagaccgcc gacggctacg cgctcgacac cagcgccgag acgctggcca ggctgaagcc
     3301 cgccttccgc gccggcggca cggtgacggc cggcaacgcc agcggcctga acgacggcgc
     3361 ggcggcgctg ctactgatgt cggccgggcg agcggcggcg ctgggcctgt cgccgctggc
     3421 gcgctgggtc ggctcggccg cggccggcgt agacccgcgc acgatgggcc tcggccccgt
     3481 gccggcaacg cgcaaggtgc tggcccgcgc cggcctgacg gtggacgaca tcgacctgat
     3541 cgaactgaac gaggccttcg ccgtgcagtc gctggcggtc atgcgcgagt tgggtctgcg
     3601 gccggagatc accaacgtca acggcggggc catcgccctg ggccacccgc tgggctgctc
     3661 cggcgcgcgc atcctgacga cgctgctgca cgagatgaag cgccgccgcg ccgccggcga
     3721 gccgatgcgc tacggcctgg cgacgctatg cgtcggcgtc ggccagggcg aggcgacgct
     3781 cgtcgagttg ctctagcgct ccgccagtgc ccgctccaca gccgccagcg cgttgacgat
     3841 gccgtagccg acgaagctat tcggctgctt cctgacaacg ccgcaggtcg gcccgtcgaa
     3901 cgcgtccggg gcgatgttga tggctgtctc ggtcagaatc tgctcggtgc ggtcgatgtc
     3961 gccgatgagc gccgggttgg ccgaccatag cagggccacc acgccgacca cgtgcgggcc
     4021 ggccatcgac gtgccgtcat aggcgacata gttgttgccg ggcacggccg acgtgacttc
     4081 cacccccggc gcggcgatgt cgggtttgat gcgtccgctg ccgtcggccg tcaccgggcc
     4141 gcgactgctg aactcggcca cgcgccgcgc ctcatcgacc gcgccgacgg tgaagacctc
     4201 gtcatagagg gccagggggc cgtcgatgct accgcacgtc gggccgttgt tgccggccga
     4261 gacgacgacg aagacccccg ccgcccgcaa ggcccgcgcc gccggcagca gtgtattgac
     4321 ctcgcagccc tcctgcggcg ggcagcccca tgagttgttg agcacgttcg cgcccaggtc
     4381 gggccggccg tcctcgaagg ggttgccacc ctgggggaag ggagccaaca ggaactgaag
     4441 acagtcgaga tagcggggcg ggttgcccag attgcgggcc aggttgacgc aggcgatcca
     4501 cgtcgcgtcg ggggcgacgc cgacgttgcg cccgacggcc gtgcccagtg tgtgcgtgcc
     4561 gtggccgccg aagtcggtcg gcgatgtcgt gccgttccac gggtcgagcc agttgtagtc
     4621 gtcgcccgtg gggccgccgg gcgtgttgcc gcggtagttg tcgtgcagtt cgaggtgggt
     4681 gacgtcggcg ccgctgtcgt tctggccgac aacgatgccc tcgccggtga cgcccagccg
     4741 gttccagacc tgcgggacgc ggataagcag ttgtgaccag agcagactgg gcggcgtaac
     4801 gtccgggttg gcctccggca gtgccggcgc gacgggcagc ggccgcacaa ccgggctgac
     4861 caggacgcgc tcgacttctg gccggcgcga aagccaccag cgcagcagcg gcccgccttg
     4921 cacttccaga ctgttgacga ggtagtaagg cgtgtagggc acgccgaagc ggtcgagcgt
     4981 ctggcgcagg tcggcctgcg tcgccgcggc gtggtgggtc aggaggtcgt agacgacggc
     5041 gcgccgctcg cccgggtcat tgatgaccac ggcgctgccg atgttggcct ggtcgcgcag
     5101 gatgacaaac agccgctcgc cgtgccagcc ggtttggccg gtcagggcgt agacaagcgc
     5161 caggccgacg acggacagaa tggccgacgc ggcccacgcc cgccggccga tggcccgccc
     5221 acccagcagc agcgccgcca ggctgagcgc ccaggccgcg gccatgccca cgatggtggc
     5281 caggaaggca taatagccca cgtcgcgata gcctagattg agggccagat ttagctcgtc
     5341 ggggtcaaaa aagagcagcg gcgcggccgt ggccagtccg accagcagcg ccgcggccag
     5401 ccacccgccc ggccgcaggc ccagccgggc cgcggcccag cccagccccg gcagcaggac
     5461 gacgagaatc agctgctgcc cgttgacgcc gaacgcggcg gccatgacgg ccaaagccac
     5521 agcggcagcc aggccgccca gggccaggcg atggccgcgg ctggaaggct cgtcgcgcaa
     5581 ggtcgccagg gtggtatgtt ccagcgtcct ggctacggcc aacccgaaga acagcgccgc
     5641 cagccaattg agcagcacg
//
LOCUS       DAOSMQ010000184         5616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14571_length_5616_cov_4.318288, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000184 DAOSMQ010000000
VERSION     DAOSMQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14571_length_5616_cov_4.318288"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PK829_07425"
     CDS             complement(<1..253)
                     /locus_tag="PK829_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOU41083.1"
                     /translation="MSPSLRPDLRAIAGLVPQGASVLDLGCGDGALLDYLKREKHARG
                     RGIELSEAGVLACVRRGLSVRQGNLQEGLADYPDGSFDCV"
     gene            complement(359..1516)
                     /locus_tag="PK829_07430"
     CDS             complement(359..1516)
                     /locus_tag="PK829_07430"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOU41084.1"
                     /translation="MTTPTDIDLVSPQTLAFAQEEPFVLESGATLRPVTLAYETYGRL
                     NADRSNAILILHALSGSAHAAGRHAPDDPRPGWWDDCIGPGKAFDTDRFFVICSNVLG
                     SCYGSSGPADIDPATGAPYGLRFPVVTIGDMVRAQTHLLDHLGIDRLLCAAGGSMGGM
                     QVLEWAAHFPHRLRAAIPLATTARHSPMLIAFSEVGRQAIYADPNWNNGEYYGNGRKP
                     DAGLAVARMVGHITYLSDASMQQKFGRRLQAREQYGYEFQTEFAVESYLKYNGNNFTR
                     RFDANSYLYITKAMDYFDLSQPTGSLAAAFTGATAVKFLVVSFTSDWLYPSYHSKELV
                     RALHAVGADVTYLDIESTWGHDAFLLEVDTMTRLLGSFLDRLAQEEGIARP"
     gene            complement(1590..2876)
                     /locus_tag="PK829_07435"
     CDS             complement(1590..2876)
                     /locus_tag="PK829_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOU41085.1"
                     /translation="MSNNERTFGFNTRQLHAGQTPDPTTGSRAVPIYQTTSYQFQNTE
                     HAANLFALKEFGNIYTRIMNPTTDVLEQRLASLEGGVGALAASSGHAAQTMAILTLCE
                     AGDHIVSSSRLYGGTYNQFNYTFPRLGIDVTFVDPAQPEAFAAAIRPNTKLIYGETLG
                     NPDISVFPFEEVSAIARRHNLPLVIDNTFATPYLCRPFEWGANIVLHSTTKFIGGHGT
                     SIGGVIVDGGNFDWTSGRFANFTTPDPSYHGLVYADLGAPAFILKARVQILRDLGACQ
                     APFNSWLTVQGIETLSLRIDRHVANAQRVAEFLENHPRINWVCYPGLRSHGDYERARR
                     VLPKGPGAILGFGIQGGRAAGESFIDHLKLFSHLANVGDARSLAIHPASTTHSQLTGE
                     EMLTAGVTPDFVRLSVGLEDIDDILWDLDQALAATA"
     regulatory      complement(3028..3146)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3239..4384)
                     /locus_tag="PK829_07440"
     CDS             complement(3239..4384)
                     /locus_tag="PK829_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU41086.1"
                     /translation="MTLTRSDYLLNPDVVFLNHGSFGATPRAVFVAYQAWQRRLEWQP
                     VQFLSVELAGHLAEARAVLGGYLHVDAGDLVYIPNATFGVNVVAHSLRLGPGDEVLTT
                     DHEYGACDRVWRYRSARQGFDYRRQSLPLPLGPAEEVVAQLWAGVTPRTRVIFLSHIT
                     SPTAARLPVEAVCARARAEGILTVIDGAHAPGQIDLDLTALGADFYTGNAHKWLSSPK
                     GAAFLYARRAVQPMVEPLVIGWGWEAEPEMSYGSRFLDYQQWLGTIDYSAYLSVPAAI
                     RFQAEHDWPTVRAACRQLVGAAIDGIGALTGLPTLYPRGGYEQMAAAALPPATDTAVL
                     KRRLLDEFAIEIPCTRWNGQPFIRVSIQAYNTAADVDALLVALRVLL"
     gene            4749..5084
                     /locus_tag="PK829_07445"
     CDS             4749..5084
                     /locus_tag="PK829_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41087.1"
                     /translation="MESNDVERRYRSHFNRTYGRRGYTFSRYLPAYRYGAKLLQQPRF
                     ANRSWSSIEKDVRRDWESRNAEPWPDFHDAVRYAWEMGQTGTQSSSKTLPTPDFSHQG
                     GPHVNKRRL"
     gene            5318..>5616
                     /locus_tag="PK829_07450"
     CDS             5318..>5616
                     /locus_tag="PK829_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41088.1"
                     /translation="MAKKKGGGILDSISDFLEDAFQGATKGEPDAPAGGTPEPVTRRV
                     SLIIHNPRVPSAGNERLNKVLAWNDPRPLLKGYMDDLRACSRGYVNYDIVERHTV"
BASE COUNT          953 a         1772 c         1956 g          935 t
ORIGIN      
        1 tgacgcagtc gaagctgcca tcggggtagt cggccaggcc ctcctgcaga ttgccctggc
       61 gcacgctgag gccgcgccgc acgcaggcca gcacgcccgc ctcgctgagt tcgatgccgc
      121 gcccgcgggc gtgcttctcg cgcttcaggt aatcgagcag cgcgccgtcg ccgcagccca
      181 ggtcaagcac gctggccccc tggggaacaa ggccggcaat ggcgcggagg tcggggcgga
      241 gggaagggga catagggggc agtgggcagt gggtcagtgg tcagtggtca gtggtcagtg
      301 ggcagtaggt cagtggtctg tcgtcggcgg tcggcggtcg tccgtcggcg gtcgtcactc
      361 acggccgcgc aatgccctct tcttgcgcca ggcggtcgag aaagctgccc agcaggcggg
      421 tcatagtgtc cacctccagc agaaaggcat cgtggcccca ggtgctttcg atgtcgagat
      481 aggtcacgtc ggccccgacg gcgtgcagcg cccgcaccaa ctccttgctg tggtacgatg
      541 ggtagagcca atcgctggtg aagctgacga cgaggaactt gaccgccgtc gctccggtaa
      601 aagccgccgc cagtgaaccc gtcggctggc tgaggtcgaa gtagtccatc gccttggtaa
      661 tgtagagata gctgttggcg tcgaagcggc gggtgaagtt gttgccgtta tacttcagat
      721 agctctctac ggcgaactcg gtctgaaact cgtacccgta ctgctcgcgg gcctgcaggc
      781 ggcggccgaa tttctgctgc atcgacgcgt cgctcaggta ggtgatgtga ccgaccatgc
      841 gggccacagc caggccggcg tcgggcttgc ggccgttgcc atagtactcg ccgttgttcc
      901 agttggggtc ggcgtagatg gcctgccggc cgacctcgct gaaggcgatc agcatcgggc
      961 tgtggcgcgc cgtcgtcgcc agggggatgg ccgcgcgcag gcgatgggga aagtgggcag
     1021 cccactccaa cacctgcatg ccgcccatgg agccgccggc ggcgcacagc agccggtcga
     1081 tgcccaggtg gtcgagcagg tgcgtctgcg cccgcaccat gtcgccgatg gtgacgacgg
     1141 ggaagcgcag accatagggc gcgcccgtgg ccggatcaat gtcggccggg ccgctggagc
     1201 cgtagcagct gcccagtacg ttgctacaga tgacgaagaa gcggtcagta tcgaaggcct
     1261 tgccggggcc gatgcagtca tcccaccagc cggggcgcgg gtcgtcgggc gcgtgccgcc
     1321 cggccgcatg ggcgctgccg ctgagggcat ggaggatgag gatggcgttg ctgcggtcgg
     1381 cattgagacg gccgtaggtc tcgtaggcca gcgtcaccgg ccgcaacgtc gccccgctct
     1441 ccagcacgaa cggctcctcc tgggcgaagg caagagtctg cgggctgacg aggtcgatgt
     1501 cggtgggagt ggtcatgggt cggggcggct ccggccgccc aagcgatggg cgacggacag
     1561 ccggtcgagg ccgtccgtcg cccatgatac tacgccgtgg ccgccagcgc ctgatccagg
     1621 tcccagagga tgtcgtcgat gtcctccaga ccgacgctga ggcgcacgaa gtcgggcgtg
     1681 acgcccgcgg tcagcatctc ttcgccggtt agctggctgt gggtcgtgct ggccgggtgg
     1741 atggccaggc tgcgcgcgtc gccgacgttg gccaggtggc tgaacagctt cagatggtcg
     1801 ataaacgact cgcccgcggc ccggccgccc tggatgccga agcccaggat ggctcccggc
     1861 cccttgggca ggacgcgcct ggcccgctca tagtcgccgt ggctgcgcag gccggggtag
     1921 cagacccagt tgatgcgcgg gtggttctcc aggaactcgg ccacacgctg ggcgttggcc
     1981 acgtgccggt ctatgcgcaa actgagcgtc tcgatgccct gcacggtcaa ccagctattg
     2041 aacggcgcct ggcacgcgcc caggtcgcgc agaatctgga cgcgcgcctt gagaatgaac
     2101 gccggcgcgc cgaggtcggc gtagaccagg ccgtggtatg aggggtcggg cgtggtgaag
     2161 ttggcgaagc ggccgctcgt ccagtcgaag ttgccgccat cgacgatgac gccgccgatg
     2221 gacgtgccgt ggccgccgat gaacttggtc gtactgtgga gaacgatgtt ggccccccac
     2281 tcgaacggcc ggcagaggta gggcgtggca aaggtgttgt cgatcaccag cggcaggttg
     2341 tgccgccggg cgatggcgct gacttcctcg aacgggaaga cgctgatgtc ggggttgccc
     2401 agtgtctcgc cgtagatgag cttcgtgttg gggcgaatgg ccgccgcgaa cgcctccggc
     2461 tgggccggat cgacgaaggt cacgtcgatg cccagacgcg ggaaggtgta gttgaactgg
     2521 ttgtaggtgc cgccgtagag ccggctgctg ctgacgatgt ggtcgcccgc ctcgcagagg
     2581 gtcaggatgg ccatcgtctg cgcggcgtgg ccgctgctgg ccgccagcgc gccgacgccg
     2641 ccctccaggc tggccaggcg ctgctccagc acgtcggtcg tcggattcat gatgcgcgtg
     2701 tagatgttgc caaactcttt caacgcgaac agattggccg catgctccgt gttctggaac
     2761 tggtagctgg ttgtctggta gatgggcacg gcccggctgc ccgtcgtcgg gtcgggtgtc
     2821 tgcccggcat gcaactggcg cgtgttgaat ccgaaagtcc gctcgttgtt actcatcgat
     2881 tcccattctc cttcgctgtg atgatggtga ccgcggcact gtcaggggga tcaaaaagac
     2941 cccgggcatc cgtttgaagc cagagggtcc gccgcggcaa agtatgctgg atgtgcgcgt
     3001 gatgggatgg ggagccaatc cctgtcgctc tcatcttcca gaacgtctgc cggaattggc
     3061 accctgccga ctggacggat ccctgccgtc cagcccgggt tgccgaggtt tcatcgggcc
     3121 ggtccctcca cctctctgga taaaagtgac agtatgcagt tgttggcggg aactatagcc
     3181 gaacggggcc ggggctgtca agtggtcaat gtgcccaaag cgcccgcggt cgccgctgtc
     3241 acaacaggac acgcaatgcc accagtagag cgtccacgtc cgcggccgtg ttgtaggcct
     3301 ggatggacac acggatgaac ggttgcccgt tccagcgcgt gcatgggatt tcgatggcga
     3361 actcgtccag cagccgccgt ttcagcacgg ccgtgtctgt cgccggcggc agcgcggcgg
     3421 cggccatctg ctcgtagccg ccgcggggat agagcgtggg caggccggtc agcgcgccga
     3481 tgccgtcgat ggccgcgccc accagttggc gacaggcagc gcgtaccgtt ggccagtcgt
     3541 gctcggcctg gaagcgaatc gccgccggga cgctcaggta ggcagagtag tcgattgtgc
     3601 ccagccattg ctggtaatcg agaaagcgac tgccgtaaga catctccggc tccgcttccc
     3661 agccccagcc gatgaccagc ggctcgacca tgggctgcac cgcccggcgg gcgtagagga
     3721 aagccgcgcc cttggggctg ctgagccact tgtgggcgtt gccggtgtag aagtccgccc
     3781 ccagcgccgt caggtcgagg tcgatctggc cgggggcgtg cgcgccgtcg atgacggtca
     3841 ggatgccctc ggcgcgggcg cgggcgcaga cggcctctac cggcaggcgc gcggccgtgg
     3901 gcgaggtgat gtggctcagg aagatgaccc gcgttcgcgg cgtgacgccg gcccacagct
     3961 gggcgacgac ctcctcggcc gggccgagcg gcaggggcaa cgactgccgc cggtagtcga
     4021 acccctggcg cgcgctccga tagcgccaca cccggtcaca ggccccatac tcgtggtcgg
     4081 tcgtcagcac ctcgtcgccc ggtcccagcc gcagcgagtg ggccacgacg ttgacgccga
     4141 aggtggcgtt ggggatgtag accagatcgc cggcatcgac gtgcaggtag ccgcccagca
     4201 ccgcccgcgc ctcggccagg tggccggcca gctccacgct caggaactgc accggctgcc
     4261 attccagccg gcgctgccag gcctgatagg cgacgaagac ggcccgtggt gtggccccga
     4321 aggagccgtg gttcaggaag acaacgtcgg gattgagcag gtagtcggaa cgagtgaggg
     4381 tcatgggcaa ggagttgggc gcgcaacggt gaagatgacc gcaataatag cgcaagccgc
     4441 agtgcctggc actttcctgt gcgcctccca ggaaggtgcg tcgcgcccac caacgccact
     4501 gcgcctggca ctttccgcag cgcgcctcta ggaaggtgcg acgcacttca gaaagtgcga
     4561 cgcacccgcg ccagaacgat ctgtatcgcg ggtacaggcc gcgctatccg ttctcagtcc
     4621 gggattgtgc gccttctatg cccggcatct atacccggca taggcgcatg gcgcggccgg
     4681 cgcgtgtaca gttagagtac gtaccgtgtt cgccgctgct gcggatgggc cggttaggag
     4741 tgtatccaat ggaatcaaac gacgtcgagc gacgctatcg gtcgcacttt aaccggacgt
     4801 acgggcgtcg gggctatacc tttagccgtt atctaccagc ttatcgctat ggcgccaaac
     4861 tgttacagca gccgcgcttc gcaaatcggt cgtggagcag cattgaaaag gatgtgcgcc
     4921 gcgattggga gagtcgcaat gccgagccgt ggccagactt ccacgacgcc gtccgctatg
     4981 cctgggaaat gggccagaca ggcacacagt cgagtagcaa aacgctgccg acgccggact
     5041 tttcacacca gggagggcca cacgtgaaca agcgccgcct gtaaccctaa gcccatctcc
     5101 tgcccccatt gcagggcttt gccaaagtca cccccggcgc gccgacctga ccggtgggcg
     5161 gccacgcctg ggactcccac cggagaacga ttggccaccc accagtcagg tcaatggtcg
     5221 cccgacgact ctggcgaagc cctgcccaca ttgacgggag cgcgccggtg gacttacaat
     5281 cagggcagat ttcaccaaca ggcaggcaag caagcacatg gccaagaaaa aaggcggcgg
     5341 tattctggat agcatcagcg acttcctgga agatgccttc cagggcgcga ccaagggcga
     5401 gccggacgcg cccgccggcg gcacgcccga gccggtgacg cggcgcgtgt cgctgatcat
     5461 ccacaacccg cgcgtgccca gcgccggcaa cgagcgcctg aacaaggtcc tggcctggaa
     5521 cgacccacgc cccctcctca agggctatat ggacgatctg cgcgcgtgta gccgcggcta
     5581 tgtcaactac gatatcgtcg agcgccacac cgtcga
//
LOCUS       DAOSMQ010000185         5581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14681_length_5581_cov_5.237423, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000185 DAOSMQ010000000
VERSION     DAOSMQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14681_length_5581_cov_5.237423"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK829_07455"
     CDS             <1..333
                     /locus_tag="PK829_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU41089.1"
                     /translation="GDLVAEDPLGTRVTAAFAGGARHIVLDLSDVPYISSAGLRAIHS
                     AYMLLRSADPADAAAATRGIATGAYKSPHLKLIRPSKNALKALSTTGYDMFLEIHDTI
                     ASAVQSFS"
     gene            330..764
                     /locus_tag="PK829_07460"
     CDS             330..764
                     /locus_tag="PK829_07460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU41090.1"
                     /translation="MKSLTLRHTTAEVRDLNDFRDFLEKAILTLGGSDDDAGDLVLAV
                     NEAVTNSLLHGYGGRPGPIGLCVEADDGELRVILYDVAAPFDPTHVPPPDINLPLEDR
                     PLGGLGIHMMRQLTDALLYHRSAQGNELTFVKRAALVGPGGD"
     gene            769..1950
                     /locus_tag="PK829_07465"
     CDS             769..1950
                     /locus_tag="PK829_07465"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU41091.1"
                     /translation="MTISTAASAPLLPPLLSPAELARWAAEGERVTVPGGEAVIREGE
                     TGDAMFILLEGELVVTVKGKRIDYLMPGAVLGEMALVDARQRSATAIAVGDAQLVRLD
                     RERFLDAIRAKPDFALRIMNMMSVRTRRLMEEEIKRQRMEEELTIGRRIQLSLLPRGC
                     PQVPGFAFAAGYRAANEVGGDLYDFIVQPDNRAKVHIVIGDVTGKGVPAALYMAVSRT
                     LMHTYALDGSSPAEALRRVNDFILHDAASPLLLSAFYGVLDADSGRLTYASAGHNPPL
                     WLHPNTGEVEPLEARGLLLGAFREFVGDEHAIVLAPGDFVVFFTDGITEARNPAGAFL
                     DDEGLEAIVRAQPWANAEELLTAIVVGVDAFAAGASQADDFTVVVLQRLPSGSSPRHV
                     R"
     gene            2014..2436
                     /gene="hypA"
                     /locus_tag="PK829_07470"
     CDS             2014..2436
                     /gene="hypA"
                     /locus_tag="PK829_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOU41092.1"
                     /translation="MLPSEPVNKAGFNEAMHELAITESILEIALRHGRSAGATRITDL
                     HLVIGELSSVVDESVQFYWDIVSEGTAAAGATLHFRRVPARLVCRACGHSYSPRQALP
                     CPACGREDISVAAGEEFYLEAIDVTSEEADAVALSETP"
     gene            2433..3104
                     /gene="hypB"
                     /locus_tag="PK829_07475"
     CDS             2433..3104
                     /gene="hypB"
                     /locus_tag="PK829_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOU41093.1"
                     /translation="MTQRVAVVERILGANDRLAADNRARLDAAGVFSLNFLASPGAGK
                     TSLVEQTVSLLAGQLRLAVIDGDIATSIDADRAAAAGATAVQINTGGECHLDAVMLQG
                     ALDQLDLTAIDLLIVENVGNLICPANFALGTHKSILVASVPEGDDKPYKYPGTYRGVD
                     ALVINKIDLLPYVRFDMDYFRRGVAALNPGLVTFPLSCRTGEGLEAWLDWVVAQVATG
                     EIARG"
     gene            3101..5464
                     /gene="hypF"
                     /locus_tag="PK829_07480"
     CDS             3101..5464
                     /gene="hypF"
                     /locus_tag="PK829_07480"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOU41094.1"
                     /translation="MTSSLIPPPSALILHPSSEAVGLRLHITGIVQGVGFRPFVYGLA
                     RRYGLTGWVRNTSSGVDIEADGPANALAGFAAALRDELPPLARIDSIDAVPRPANGYT
                     AFDIRASQAEPGAFQPIAPDVAVCDDCLRELFDPADRRYRYPFINCTNCGPRFTIIRD
                     IPYDRPLTTMAGFPLCPDCAAEYADPLDRRFHAQPVACPACGPRLWLERPGAAGELAA
                     GDDALAMTRTLLGEGGIIAIKGLGGFHLACDATDAAAVAELRRRKLRVGKPFAVMFPD
                     LAAARRACELSAAEEALLLSPARPIVVARRRPGAAIAAEVAPGQATIGILLPYTPLHH
                     LLLERAPGFPEALVMTSGNLSEEPIAFTNAAACERLAGLADAFLLHNRDIYIRCDDSV
                     SRVVPGSGAAVPLRRSRGYTPYPVRLPRPASPLLATGAELKNTFCLTRGDYAFLSHHI
                     GDLENYETLVAFEEGVAHMERLFRVRPEALAYDLHPDYLATRYALERAGREGLPAVGV
                     QHHHAHIAAGMAEHALPPDTRVIGVAFDGTGYGDDGAIWGGEFLLAGYGDYERAAHLR
                     YTPLPGGDRAVREPWRVALAWLATAGLPWDDDLPPVAHARATRPDGLAALAQQIARGI
                     NSPPTSSMGRLFDAAAALAGVRQAVTYEAQAAIEFEALADGDETAIYPFDYTNDIIDP
                     APLWAALLADRRAGLPLPRLAARFHNGVAHMTRDVCRHLRATHGLDQVVLSGGVWQNM
                     TLLRATLALLAADGFTVYSHRLVPPNDGGLALGQAAVATAIRMRGEG"
BASE COUNT          846 a         2095 c         1750 g          890 t
ORIGIN      
        1 ggcgatctcg tcgccgaaga cccattggga acgcgcgtga ccgcggcttt cgccggtggg
       61 gcgcgccaca tcgtgctcga cttgagcgat gtgccctaca tcagcagcgc cggcctgcgg
      121 gccatccact ccgcctacat gctcttgcgc agcgccgacc cggccgacgc cgccgcggcg
      181 acgcgaggca tcgccacggg tgcctacaaa tcgccccacc tcaagctcat ccggccgtcg
      241 aaaaacgccc tgaaggcgct gtccacaacc ggctacgaca tgttcctcga aatccacgac
      301 accatcgcta gcgccgtcca atcgttctca tgaaatccct caccctgcgc cacacgacgg
      361 ccgaggtgcg cgacctgaac gactttcgcg acttcctgga gaaggcgatc ctgaccctcg
      421 gcggcagcga cgacgacgcc ggtgacctgg tgctggcggt caatgaggct gtgaccaaca
      481 gcctgctcca cggctacggc ggccggcctg gcccgattgg cctctgcgtc gaggccgacg
      541 acggcgagct gcgcgtcatc ctctacgacg tggctgcgcc gtttgacccc acgcacgtgc
      601 caccgccgga catcaacctg cccctcgagg atcggccgct cggtggcctg ggcatccaca
      661 tgatgcgcca gctgaccgat gcgcttctct accatcgctc ggcccagggc aacgagttga
      721 ccttcgtcaa acgcgctgcc ctcgtcgggc cagggggcga ctaaccccgt gacgatctcc
      781 accgcggcca gcgcgcccct tctgccgccg ctcctgtcgc cggccgagct ggcgcgttgg
      841 gccgcggaag gggagcgcgt gaccgtgccg ggcggcgagg ccgtcatccg cgagggcgag
      901 accggcgacg ccatgttcat cctcctggaa ggcgaactgg tcgtcactgt caagggcaag
      961 cgaattgact acctgatgcc cggcgccgtc ctgggcgaga tggctctggt cgatgcccgg
     1021 caacggagcg ccacggccat cgccgtcggc gacgcgcaac tcgtccgcct cgaccgcgag
     1081 cgctttctgg acgccatccg cgccaaaccc gattttgccc tgcggattat gaacatgatg
     1141 tccgtgcgca cccgccggct gatggaggag gagatcaagc ggcaacgcat ggaggaggag
     1201 ctgaccattg gccgccgcat tcagctcagc ttgctgccac gtggctgccc acaggtgccc
     1261 ggctttgcct tcgccgccgg ctatcgcgcc gccaatgagg tcggcggcga cctgtatgac
     1321 ttcatcgtcc agcccgacaa ccgcgccaag gtccacatcg tcatcggcga cgtgaccggc
     1381 aaaggcgtgc ccgcggcgct gtatatggcc gttagccgca cgctgatgca cacctacgcc
     1441 ctcgacggct ccagcccggc cgaggcgctg cggcgggtca acgactttat cctccacgac
     1501 gccgcctcgc cgctgctgct cagcgcgttt tatggcgtgc tcgacgccga ctctggccgc
     1561 ctgacctacg ccagcgccgg ccacaacccg cccctctggc tccacccaaa cacgggcgaa
     1621 gtggagccgc tggaggcgcg cggcctgcta ctgggcgcgt tccgcgaatt cgtgggcgac
     1681 gagcacgcca tcgtcctggc ccccggcgac ttcgtcgtct tcttcactga cggcatcacc
     1741 gaggcccgca acccggccgg cgcttttctg gacgacgagg ggctggaggc catcgttcgc
     1801 gcgcagccgt gggccaatgc cgaggaattg ctgaccgcca ttgtcgtggg cgtggacgcc
     1861 ttcgccgccg gcgcgtccca ggctgacgac ttcaccgtgg ttgtcttgca gcgcttgccc
     1921 tctggcagct ccccccgcca cgttcgctaa atcgtgatct tgtcgtgcca aaagcatgac
     1981 agatgtcact gcccaggccc ggcggccggg tgtatgcttc cttctgaacc tgtcaacaag
     2041 gccgggttca acgaagccat gcacgaattg gccatcaccg agagcatcct ggagatcgcc
     2101 ctgcgccacg gccgcagcgc cggcgcaacg cgcatcaccg acctgcacct ggtcatcggc
     2161 gagctgtcga gcgtcgtcga tgagtcggtg cagttctact gggacatcgt cagcgaaggc
     2221 accgccgccg ccggggcaac gctccacttc cggcgcgtgc ccgcgcggct cgtctgccgc
     2281 gcctgcggcc acagctatag cccgcgccag gccctgccct gccctgcctg tggccgcgaa
     2341 gacatcagcg tggccgcggg cgaagagttc tatctggaag ccatcgacgt aacctccgag
     2401 gaggccgacg ccgtggcgct aagcgagaca ccatgaccca acgagtagcc gttgtagagc
     2461 gcatcttggg cgccaacgac cgcctggcgg ccgacaatcg cgcccgcctc gacgccgccg
     2521 gcgtcttcag cctcaacttc ctggcctcgc ccggcgcggg caagaccagc ctcgtagagc
     2581 agactgtcag tctgctcgcc ggccagttgc gcctggctgt catcgacggc gacatcgcca
     2641 ccagcattga cgccgaccgc gccgccgccg ccggcgccac ggccgtgcag atcaacaccg
     2701 gcggcgagtg ccacctggac gcggtcatgc tccagggcgc gctggatcag cttgacctga
     2761 ccgccattga cttgcttatc gtcgagaacg tcggcaatct catctgcccg gccaacttcg
     2821 ccctgggaac gcacaaaagc atcctcgtgg cctcggtgcc ggagggggac gacaagccct
     2881 acaagtaccc cggcacctac cgcggcgtcg atgccctggt catcaacaag atcgacctgc
     2941 tgccctacgt ccgcttcgac atggactact tccgccgcgg cgtggccgcg ctgaaccccg
     3001 gcctggtcac cttccccctg tcctgccgca ccggcgaagg gctggaggcg tggctcgatt
     3061 gggtcgtggc ccaggtggcg acaggagaaa tagcccgcgg atgacttcat ccctcatccc
     3121 tcctccctca gccctcatcc ttcatccttc atccgaagcg gttggcctgc gcctccatat
     3181 caccggcatc gtccagggcg tgggcttccg gccgtttgtc tacggtctgg cccggcgcta
     3241 tggcctgacc ggctgggtgc gcaacacctc ctccggcgtc gacatcgagg ccgacggccc
     3301 ggccaacgcg ctggcgggct ttgccgcggc gctgcgcgac gagttgccac cactggcgcg
     3361 catcgacagc attgacgccg tgccgcgccc ggccaacggc tataccgcct tcgacatccg
     3421 cgcttcccag gccgagccgg gcgcgttcca gcccatcgcg cccgacgtgg ccgtctgcga
     3481 cgactgtctg cgcgagctat tcgacccggc cgaccgccgc taccgctacc cgttcatcaa
     3541 ctgcaccaac tgcggcccgc gcttcaccat cattcgcgat atcccctacg accggccgct
     3601 gacgacgatg gccggcttcc cactctgccc cgactgcgcc gccgagtacg ctgacccgct
     3661 cgaccgccgc ttccacgccc agcctgtcgc ctgccccgcc tgcgggccgc ggctgtggct
     3721 ggagaggccg ggcgcggccg gggaactggc cgccggcgac gacgcgctag ccatgacacg
     3781 gacgctgttg ggcgagggag gcattatcgc catcaagggg ctgggcggct tccacctggc
     3841 ctgcgacgcg accgacgccg ccgccgtggc cgagttgcgc cgccgcaagt tgcgcgtcgg
     3901 caagccgttc gccgtcatgt tccccgatct ggccgccgcc cgccgcgcct gcgaattgag
     3961 cgcggccgag gaggcgctgc tgctgtcgcc ggcgcggccg atcgtcgtcg cccgccgccg
     4021 ccccggcgcg gccattgccg ccgaagtcgc ccccggccag gccaccattg gcatcctgct
     4081 gccctatacg cccctccatc acctgctgct ggagcgcgcc cccggcttcc ccgaagcact
     4141 ggtgatgact agcggcaatc tcagtgagga gccgatcgcc ttcaccaacg cggccgcctg
     4201 cgagcggctg gccgggctgg ccgacgcctt cctgctccac aaccgcgaca tctacatccg
     4261 ctgcgacgac tccgtgtcgc gcgtcgtgcc cggcagcggc gcggccgtgc ccctgcgccg
     4321 gtcgcgcggc tacacgccct accccgtgcg cctgccgcgg cccgcgtcgc cgctgctggc
     4381 cacgggcgcg gaactgaaga acaccttctg cctgacccgc ggcgactacg ccttcctcag
     4441 ccaccacatc ggcgacctgg agaactacga gacgctggtg gccttcgagg agggcgtggc
     4501 ccacatggag cgccttttcc gtgtccggcc ggaggcgttg gcctacgacc tgcaccccga
     4561 ctacctggcc acacgttatg ccctggagcg ggccggacgc gaggggttgc cggccgtcgg
     4621 cgtgcagcac caccatgccc acattgccgc gggcatggcc gagcacgctt tgccgcccga
     4681 tacccgcgtc atcggtgtgg ccttcgacgg cacgggctat ggcgacgacg gcgcgatctg
     4741 gggcggcgag ttcctgctgg ccggctacgg cgattacgag cgggccgccc atctacgcta
     4801 tacgcctctg cccggcggcg accgcgccgt gcgcgagccg tggcgcgttg ctctggcctg
     4861 gctggccacg gccggcctgc cctgggacga cgacctgccg cccgtggccc acgcgcgcgc
     4921 cacgcggccc gatggcctcg ccgcgctggc ccagcagatc gcccgcggca tcaatagccc
     4981 gccaacatcg agtatgggcc gcctgttcga cgcggccgcg gcgctggccg gcgtgcgcca
     5041 ggctgtgacc tacgaggccc aggcggccat cgagttcgaa gccctggccg acggtgacga
     5101 gacggctatc tatccctttg actatacgaa tgacatcatc gaccccgcgc cgctctgggc
     5161 cgccctgctg gccgaccgcc gcgccgggct gcccctgccc cgtctggccg ctcgcttcca
     5221 taatggcgtc gcccacatga cccgcgacgt ttgccgccac ctccgcgcca cccacggcct
     5281 cgatcaagtc gtcctcagcg gcggcgtgtg gcagaacatg accctgctgc gggcgacgct
     5341 tgccctgctg gccgccgacg gcttcaccgt ctacagtcac cggctcgttc cgcccaacga
     5401 cggcggcctt gctcttggcc aggcagccgt ggccacggct ataaggatga ggggtgaagg
     5461 atgaaggatg acaggcagtc gtgggcagtg tacagtggcc agtgtacagt gtacagtggc
     5521 cagcggtgat ctgccctgaa aaaagtggac acacaattaa ggtggaacga tactaacctt
     5581 g
//
LOCUS       DAOSMQ010000186         5577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14693_length_5577_cov_4.967041, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000186 DAOSMQ010000000
VERSION     DAOSMQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14693_length_5577_cov_4.967041"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PK829_07485"
     CDS             complement(<1..714)
                     /locus_tag="PK829_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU41095.1"
                     /translation="MTDLLIDNGHLVTWMAADEILADNALLLRDSRIHDIGPSATLRA
                     RYPDAERLDARGQLVMPGNICAHTHFYGAFARGLAIPGPAPRDFPDILERLWWRLDRA
                     LLDVDVAYSALVSLVDAIKHGTTTLVDHHASPNAIDSSLDQIADAVETAGVRAVLCYE
                     VTDRNGPAGAQAGIGENVRFLHAVAERPDNLLRATFGLHASLSLSDRTLERCVAAAEG
                     LGTGFHIHVAEHEVDEYDSR"
     gene            1066..1404
                     /gene="trxA"
                     /locus_tag="PK829_07490"
     CDS             1066..1404
                     /gene="trxA"
                     /locus_tag="PK829_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU41096.1"
                     /translation="MAKPLDVTDADFQTEVLESNMPVLVDFWADWCHPCRIIAPFVKE
                     IAAEQEGVLKVAKLDVDENPAVPGRYGIVGIPTLMLFKGGKVVERIVGAQPKERILAQ
                     VMPHLEKAAA"
     gene            1468..2382
                     /locus_tag="PK829_07495"
     CDS             1468..2382
                     /locus_tag="PK829_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOU41097.1"
                     /translation="MEIALMIEGQDGLTWPRWQRLAAAAEDLGFVGLYRSDHYTNANP
                     PDKASLELWVSLTWLASHTGRIEFGPLVSPVSFRQPTLTARMAAAVDDLSGGRLTLGL
                     GAGWQAREHDHFGWQLLDVPARFARFEEGLEVITRLLNSDAPVDFDGAHYRLREAILL
                     PRPARPGGPPILIGGNGPKRTLPLVARYASEWNGVFLTPADFADRNRQLDALLLAAGR
                     EPAAVRRSLMIGCVFGRDAAELEAHAARRGTTPDKLRASGKLAVGDGAEIADQLVAWS
                     AAGVQRIMLQWLDLDDLDGIAALARAIL"
     gene            complement(2475..2945)
                     /locus_tag="PK829_07500"
     CDS             complement(2475..2945)
                     /locus_tag="PK829_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41098.1"
                     /translation="MTDPNIDNLKNEERLKIDIPVEEEMSGRAAKTSPMTTDLVEEFK
                     RLGRQFGDTVQTALNSEEARRLESELRAGMRSFADEVERAFRDAKTNPTATRMKEEAT
                     GVKERVETGEMARKAQEGIVSSLRWLSAELDQLATQFTPAQQTPPPTEKQPPTE"
     gene            complement(3027..4016)
                     /locus_tag="PK829_07505"
     CDS             complement(3027..4016)
                     /locus_tag="PK829_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41099.1"
                     /translation="MQSPQLLRRVVVLATLALAVVMLAGCGRETPAAEATPIAAAVTS
                     APAATWTPLPAAVVVTNTPAPTSPPPASPTPAPSATPLPASPTAESSPTPAASPTLAA
                     TATAIPPTAAPATAGPTRPPATAAPPTLPPNPTLGQNLLPNPSFEEGFSNQNGIAELH
                     APNGWRIEYQEGPTGFGTNAWDTYVRPDTRVFPAAFLPPEEHSLYIFDGSTTLKVFRE
                     QGALSMRLLTDVQLGPGTYVLEANFFSDVFERYEDDHKVAPSDPNAGEAALFAGTGGT
                     GWIANNYLTKNTLTHTFTISSAQTMTVGVGFRGRYAIANNGWFVDNLSLRQLQ"
     gene            4227..5144
                     /locus_tag="PK829_07510"
     CDS             4227..5144
                     /locus_tag="PK829_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="R2-like ligand-binding oxidase"
                     /protein_id="HOU41100.1"
                     /translation="MSHTSFATTSRGLRRDVPAMRLYEKAKRLGIWNPSDIDLTADRQ
                     HWLALADDERDILLRLTAMFQAGEEAVTLDLLPLIMTIAAEGRVEEEMFLTTFLFEEA
                     KHTDFFSRFLTNVAVSTTSSTAAGALGRYHSDNYRAIFYDVLPATLGALAADRSPAAQ
                     VRAAVTYNMIVEGMLAETGYHAYFTVLERHDLMPGTRRGIGLLKQDESRHIAYGIYLL
                     SRLVAEDAGLWDVLEEQMNALLPAALGVIGDVFAAYDPVPFGLVEEDFIDYGLAQFQK
                     RVARLERARGMSLAEIMRDTNRVIERDDA"
     gene            complement(5280..>5577)
                     /locus_tag="PK829_07515"
     CDS             complement(5280..>5577)
                     /locus_tag="PK829_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HOU41101.1"
                     /translation="YVNAFEKLRDPSHVRCLSEEEWLDALAAAGLAVAARETLDKRLT
                     FETWAARHTPLMQTRLRAMLLQAPEAARAFLDPRPEGVTTFRLREGLFIARRSL"
BASE COUNT          891 a         1803 c         1917 g          966 t
ORIGIN      
        1 gcgcgaatcg tactcatcga cctcgtgctc ggccacgtgg atgtggaagc cggtgcccag
       61 gccctcggcc gcggccacgc agcgctccaa cgtccggtcg ctcagcgaca gcgaagcgtg
      121 cagaccaaag gtggcccgca gcaggttgtc ggggcgctcg gccacggcat ggaggaagcg
      181 cacgttctca ccgatgcccg cctgtgcccc ggccgggccg ttgcggtcgg tcacctcgta
      241 gcacaaaacg gcgcgcacgc cggccgtctc cacggcatcg gcgatctggt ccagcgaact
      301 gtcgatggca ttggggctgg cgtggtggtc gaccagcgtc gtcgtgccat gcttgatggc
      361 atcgaccagg ctgacgaggg cactgtaggc cacgtccaca tcgagcaatg cccggtcgag
      421 ccgccaccac agtcgctcca ggatgtcggg gaagtcccgg ggcgcggggc cagggatggc
      481 caggccgcgg gcgaacgcgc cgtagaagtg cgtatgggcg cagatattgc cgggcatgac
      541 cagttggccg cgggcatcca ggcgctcggc gtcggggtag cgcgcgcgca gggttgccga
      601 cgggccgatg tcgtggatgc ggctgtcgcg cagcagcaag gcgttgtcgg ccaggatttc
      661 gtctgctgcc atccaggtga ccaggtggcc gttgtcgatg aggaggtcgg tcatggcaca
      721 attctagccg ccgcgcgccg gtgggcaagc tgagtccagg gctttgccaa agtcgtctag
      781 cgactattga cctgaccggt gggcggccag tcattcgttg gtggtcgccc acttgtcagg
      841 tcggcgcgcc gggggtttga cttcggaaaa gccctgaagc tgagttgtca gacctgtctg
      901 gccagccgcc ttttctgaga ggaatatgca caacgacggg gattccacta taatgcgcgc
      961 catactaatg tctttctgac actcttgggt aagaatcgac aatacggtcg cgtctacggc
     1021 ctaaggcaac gaattgagaa ttactctgaa ggagataaaa ggataatggc caagccactt
     1081 gatgttactg acgccgactt tcagaccgaa gtcctggagt ccaatatgcc cgttctggtt
     1141 gatttctggg cggactggtg tcatccttgc cgcattattg ccccgttcgt caaggaaatc
     1201 gccgcggagc aggaaggcgt cctgaaggtc gccaagctcg acgtggacga gaatcccgcc
     1261 gtgcccgggc gctatggcat cgtcggcatc ccgaccctga tgctcttcaa gggtggcaag
     1321 gtcgttgagc gcatcgttgg cgcccagccg aaggagcgca tcctggccca ggtgatgccg
     1381 catctggaaa aggccgcggc ctaagtcaga ccgaaatgag agggcgtcgc cgtggggcga
     1441 cgcccttttt tgttggcgag ggctactgtg gaaatagcgc tcatgatcga gggccaggac
     1501 ggcctgacct ggccgcgctg gcaacggctg gcggccgccg ccgaagacct cggcttcgtt
     1561 ggcctctacc gctccgacca ctacaccaac gccaacccgc ccgacaaggc atcgctggaa
     1621 ctgtgggtct cgctgacctg gctggccagc cacaccgggc gcatcgaatt cggcccgctc
     1681 gtctcgcccg tgtccttccg ccagcccacg ctgaccgcgc gcatggccgc ggccgtcgat
     1741 gacctgtccg gcgggcggct gacgctgggc ctgggcgcgg gctggcaggc gcgcgagcac
     1801 gaccatttcg gctggcaact gctcgacgtg ccggcgcgct ttgcccgctt cgaggagggc
     1861 ttagaggtca ttactcgcct gctgaacagc gacgcgccgg tggacttcga cggcgcgcac
     1921 taccgcctgc gcgaggccat cctactgccg cgaccggcgc gccccggcgg cccgcccatc
     1981 ctcatcggcg gcaacgggcc aaagcggacg ctgccgctcg tggcccgcta cgccagcgag
     2041 tggaacggtg tcttcctgac cccggccgac tttgccgacc gcaaccggca actggacgcg
     2101 ttgttgctgg ccgcggggcg cgagccggca gcggtgcgcc gctcgctgat gatcggctgc
     2161 gtctttggcc gcgacgccgc cgagctggag gcccacgccg cccgccgcgg cacgacgccg
     2221 gacaagctgc gcgcctccgg caagctggcc gtgggcgatg gcgcagagat tgccgaccag
     2281 ctggttgcct ggtcggccgc cggcgtccag cgcatcatgc tccagtggct cgacctggac
     2341 gacctcgacg gcatcgccgc gctggccagg gccattctct agccgccacc cgcccggaca
     2401 gctctgccaa taccaaagac gcccggcaga ccatctgccg ggcgtctttg atcgttgcta
     2461 attgggagtg ttgcttactc ggtcggcggc tgcttctcgg tcgggggcgg ggtctgctgg
     2521 gcaggcgtga actgcgtggc cagctgatcg agctcggcgc tcagccagcg caggctgctg
     2581 acaatgcctt cctgcgcctt gcgggccatt tcgcccgtct cgacacgctc cttcacgccg
     2641 gtggcctctt ccttcatacg cgtggccgtt gggttggtct tggcatcgcg gaaggcgcgc
     2701 tctacctcgt cggcgaaact acgcatcccg gcgcgcagtt ccgactccag ccggcgggct
     2761 tcctcgctgt tcaaggccgt ctgcacggtg tcgccaaact gccggccgag gcgcttgaac
     2821 tcctccacca ggtcggtcgt catgggcgag gtcttggccg ccctgccgga catctcttcc
     2881 tcaacgggga tatcaatctt cagccgctct tcgttcttca ggttgtcgat attcgggtca
     2941 gtcatcactt gcgctccttg tcagatagac gttgagtcta taattaccaa actatacggc
     3001 ccaacggccg cgtagttgca gccagactac tgtagctgcc gcagggacag gttgtccacg
     3061 aaccagccgt tgttggcgat ggcgtagcgt ccgcggaagc cgacgccgac agtcatcgtc
     3121 tgcgcgctgc tgatagtgaa ggtgtgggtc agggtgttct tggtcagata attgttggcg
     3181 atccagcccg tgccgcccgt gccagcaaaa agggccgcct cgcccgcgtt ggggtcgctt
     3241 ggcgcaacct tgtggtcgtc ttcgtatcgc tcaaagacat ccgagaagaa gttggcctcc
     3301 agcacatatg tgcctggccc cagttgtacg tcggtcagta gacgcatgct cagcgccccc
     3361 tgctcgcgga agaccttcag agttgtggag ccgtcgaaga tatagaggct gtgctcctcc
     3421 ggcggtagga aggcggccgg gaagacgcgc gtgtcgggcc gcacgtaggt gtcccaggcg
     3481 ttcgtgccga agccagtcgg cccttcctgg tactcgatgc gccagccgtt gggcgcgtgc
     3541 aattcggcga tgccgttctg gttactgaag ccctcctcga acgaggggtt gggcagcaag
     3601 ttttggccca gcgtcgggtt gggcgggagg gttggcggcg cggccgtggc cggggggcgc
     3661 gtgggcccgg cggtggccgg ggcggccgtg ggcgggatgg ccgtggccgt cgccgcgagc
     3721 gtgggcgagg cggccggcgt cggtgaggac tccgccgtgg gcgaagcggg cagcggggtg
     3781 gccgatggcg cgggcgtggg cgaggccggc ggcggcgacg tgggcgcggg cgtgttggtc
     3841 acgacgacag ccgcgggcag gggtgtccac gtggccgccg gggcagaagt gacggccgcg
     3901 gcgatgggcg tggcctcggc cgccggcgtc tcgcgcccgc agccggccag catgacgacg
     3961 gccagcgcca gcgtcgccag gacgaccacg cgacgcaaaa gttggggtga ttgcacggac
     4021 ttcctccagg caattgggtt gcgctgccgg gcagccaccc ggcagcgatt ggattgtatg
     4081 gccgcggaca gaggcgggca acggccgagg cggagggata gcgggattct catctgtcga
     4141 gtcgcaatca gctttcgggc aggggcacag gagagtgggg cgcacaagga gtgcgtcacc
     4201 cgggaagcga ggctagaatc gcggccatgt cccacactag cttcgccacc acgtcccgcg
     4261 ggctgcgtcg cgacgtgcca gccatgcgcc tctatgagaa agccaagcgc ctgggcatct
     4321 ggaaccccag cgacatcgac ctaaccgctg accggcagca ctggctggcc ctggccgacg
     4381 acgagcgcga catcctgttg cgcctgacag ccatgttcca ggccggcgag gaggccgtca
     4441 ccctcgacct gctgccgctg atcatgacca tcgccgccga gggacgtgtc gaggaggaga
     4501 tgttcctgac cactttcctg ttcgaggagg ccaagcacac cgacttcttc agccgcttcc
     4561 tgaccaacgt agccgtcagc acgaccagca gcacggccgc tggcgctctg gggcgctatc
     4621 acagcgacaa ttaccgcgcc atcttctacg acgtactgcc ggcgacgctg ggcgcgctgg
     4681 cggccgatcg ctcgccggct gcccaggtgc gcgccgccgt cacctataac atgatcgtcg
     4741 aggggatgct ggccgagacg ggctaccacg cctacttcac cgtgctggag cgccacgacc
     4801 tcatgcccgg cacgcggcgc ggcatcggcc tgctgaaaca ggatgagtcg cgccatattg
     4861 cctacggcat ctacttgctg tcgcggctgg tagctgagga cgcgggcctg tgggatgtgc
     4921 tggaagagca aatgaacgca ttgctgccgg ctgcgttggg cgtcatcggc gatgttttcg
     4981 cggcctacga tcccgtgccc ttcggcctgg tggaggagga cttcatcgac tacggcctgg
     5041 ctcaattcca gaagcgcgtc gcccgcctgg agcgagcgcg gggtatgtcg ctagcggaga
     5101 ttatgcggga caccaaccgg gtcatcgaac gcgacgacgc ctgaccgtag cacaggctgt
     5161 tagcctgtct ccccgcggca ggctaacagc ctgcgctaca gggcgcggca ggctaacagc
     5221 ccgcgctaca gggcgcggca ggctaacagc ctgcgctaca gcctgcgcta caggctgcgc
     5281 tacaggctgc ggcgggcaat gaataacccc tcgcgcagcc ggaaggtggt cacgccctcg
     5341 gggcgcgggt caaggaacgc acgcgccgcc tccggggctt gcagcagcat cgcccgcagt
     5401 cgcgtctgca tcagcggcgt gtggcgggcg gcccacgtct cgaatgtcag ccgcttgtcc
     5461 agcgtctccc gcgccgccac ggccagcccg gcggcggcca gcgcgtcgag ccactcctcc
     5521 tcgctcaggc agcggacgtg gctggggtcg cgcagcttct cgaaggcgtt gacgtat
//
LOCUS       DAOSMQ010000187         5549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14780_length_5549_cov_4.201675, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000187 DAOSMQ010000000
VERSION     DAOSMQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14780_length_5549_cov_4.201675"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..2510
                     /locus_tag="PK829_07520"
     CDS             33..2510
                     /locus_tag="PK829_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU41102.1"
                     /translation="MMRFDRFTERAQDAAARAYEIVQRYGHSQVDTEHVFLALLEQQD
                     GAVPQILASLNADTAVLQERLDRELRASPRVSVYGGGVGQVYYTPRIRTVLELSQGEA
                     TRLKDEFISTEHIFLAILSERNTPSARIVSELGISREAVLNAIMELRGGQRVTSRQAE
                     SRYRTLEKYSRDLTQMAREGRLDPVIGRDAEILRVIQVLSRRTKNNPVLIGEAGVGKT
                     AIVEGLAQKIIISDVPENLLNKRVVALDLGAMIAGSRFRGEFEERLKASMEEIQRAQG
                     EIILFIDELHTVVGAGSAQGAMDASNMMKPALARGELQCVGATTLDEYRQYVERDAAL
                     ERRFAPVFVDEPSVDETIDMLRGLRDRYEAHHSVQFSDAAIVAAVKLSSRYLTDRRLP
                     DKAIDLMDEAAAKLRVALHTLPPDLKAKKTAIDKLLASEEEASAARDYERAQVSKAER
                     LRLETEFHALRDAWRGEQRLDETVDERDVAEVIASWTGIPVSQMMETEAQKLLHMEER
                     LHERIVGQERAIAALSDAIRRSRSGLSDPRRPIGSFIFLGSSGVGKTELAKALAEFLF
                     DDEEALIRVDMSEYREQHTVSRLFGAPPGYVGYEQGGQLTEQIRRRPYSVVLFDEIEK
                     AHPDIWNALLQLLDDGRLTDGQGRVVNFRNAVIVMTSNVGTSFVKRSGALGFAGMAAA
                     EREDHKRIEEALKQTFRPEFINRIDEVIIFEPLSEVDVVEIVKLQVKDVQMRLAEHGN
                     LSIELTETAQRWLAAQGFDKDFGARPLKRAIQRYVESPLSMRLLRGEFKSGDRVRIDV
                     ADDQLDFILLESGPVPQKAVEEMAEHA"
     gene            complement(2593..3024)
                     /gene="arfB"
                     /locus_tag="PK829_07525"
     CDS             complement(2593..3024)
                     /gene="arfB"
                     /locus_tag="PK829_07525"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOU41103.1"
                     /translation="METDLIPITEALSLPLAELVFRFTTGGGPGGQHVNKTATRVTLL
                     FDVARTPSLDEATRARLLDRLANRLDRRGVLHIDVQDSRSQWQNRELAIARLQSLLAG
                     ALAEQAERRPTRPSRRARQARLDDKRRHSTVKRERQQRWED"
     gene            3082..4038
                     /locus_tag="PK829_07530"
     CDS             3082..4038
                     /locus_tag="PK829_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloenzyme domain-containing protein"
                     /protein_id="HOU41104.1"
                     /translation="MPGQVTTPDRAHVHIFFMDGVGLGAADPAENPFAAARLLNLTAL
                     LGAEWFVGGRGPVAAARATLVPTDATLGLPGRPQSATGQATILTGRNVPALVGEHYGP
                     KPNAAVANIVRQGTLFSEVAAAGGRAALLTPYPERYFEAINSGRRLLSAVPLAAASAG
                     LPLLGVDDLRAGRAVSPDFTGAGWRDFLGYADVAVLSPEDAGQRIAALAAAHDFSFFE
                     HWPTDQVGHRGSLAEAVAHLESMDAALGGLLDAWDDAHSLLILTSDHGNLEEKSHRQH
                     TRNAVPTILVGRDHAAHAATIHDLTDIAAVVRRVLGLPTPNY"
     gene            4055..4546
                     /locus_tag="PK829_07535"
     CDS             4055..4546
                     /locus_tag="PK829_07535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOU41105.1"
                     /translation="MPITSESFRDVLRHFPSGVTVVTIQSPASDVVHGLTVSAFASVS
                     PEPPLVLVSIDHRTTAYMLLEAAGASFAVNILAHDQMELSNRFAWLKDEDRFQIGDWT
                     TAVTGAPILREAVAWLDCTVYSRFAAGTHTIYIGEVQASGVPRPDVKPLLYWNRGYRQ
                     MSK"
     gene            complement(4560..5096)
                     /locus_tag="PK829_07540"
     CDS             complement(4560..5096)
                     /locus_tag="PK829_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOU41106.1"
                     /translation="MGKATEENWPVQIIRSAQRRKTVSARLEGGVLVIRAPEGLSDAD
                     LAPIIDNLRQRMRRRGRSEPLSDEALEKRAEALNRKYFDGRLRWQSIRYVTNQNTRFG
                     SCTPSDGTIRLSHRLASMPAWVSDYVLIHELAHLVERNHGKRFWKLVNRYPLTERARG
                     YLMAVGLEELEMGPSDET"
     gene            complement(5168..5509)
                     /locus_tag="PK829_07545"
                     /pseudo
     CDS             complement(5168..5509)
                     /locus_tag="PK829_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
BASE COUNT          911 a         1827 c         1872 g          939 t
ORIGIN      
        1 ggtggcggag ccaccttata aggataaaca caatgatgcg atttgatcga tttaccgaac
       61 gcgcccagga tgccgccgcg cgcgcttatg agatcgtcca gcggtatggt cactcgcagg
      121 tcgataccga gcacgtcttc ctggcgctgt tggagcaaca agacggcgct gttccccaga
      181 ttctggcatc cctgaacgcc gacacggccg tgctgcaaga gcggctcgac cgcgaactgc
      241 gggccagccc gcgggtttcg gtctatggcg gtggcgtggg gcaagtgtac tatacgccgc
      301 gcatccgcac tgtcctggag ctttcccagg gcgaggcgac gcggttgaag gatgagttca
      361 tctccaccga acacatcttc ctggctatcc tcagtgagcg caatacgccc tcggcccgca
      421 tcgtcagcga gctgggcatc agccgcgagg ccgtgctgaa cgccatcatg gagttgcgcg
      481 gcggccagcg cgtgacctcg cgccaggccg aaagccgcta tcgcacgctg gagaagtaca
      541 gtcgtgacct gacccagatg gctcgcgagg gtcgcctcga cccagttatc ggccgcgacg
      601 ccgaaatcct gcgcgtcatc caggttctta gccggcgcac gaagaacaat cccgtgctca
      661 tcggcgaggc cggcgttggt aagacggcca tcgtcgaggg actggcgcag aagatcatca
      721 tcagcgacgt gccggagaac ctgctcaaca agcgcgtcgt agccctcgac ctgggggcca
      781 tgatcgccgg cagccgcttc cgcggcgagt tcgaggagcg gctcaaggca tcgatggagg
      841 agattcagcg cgcccagggc gagatcattc ttttcatcga cgagctgcat actgtcgtcg
      901 gcgcggggtc agcccagggg gccatggacg ccagcaacat gatgaagccg gcgctggccc
      961 gcggcgaact gcaatgcgtc ggcgcgacca cgctggacga gtatcgccag tacgtcgagc
     1021 gcgatgctgc cctggagcgc cgcttcgcgc ccgtgttcgt cgatgagcct agcgtggacg
     1081 agaccatcga catgttgcgc ggcctgcgcg atcgctacga ggctcatcat agcgtgcagt
     1141 tctccgacgc ggccatcgtg gccgccgtca agctgtcgtc gcgctacctc accgaccggc
     1201 ggctgcccga caaggccatc gacctgatgg acgaggctgc ggccaagctg cgcgtggcgc
     1261 tgcatacgct gccgcccgac ctgaaagcca agaagacggc catcgataag ctgctggcca
     1321 gcgaggaaga agccagcgcc gcccgcgact acgagcgcgc ccaggttagc aaggccgaac
     1381 ggctccgtct ggaaacggag ttccacgccc tgcgcgacgc ctggcgcggc gaacagcggc
     1441 tggacgagac cgtcgatgag cgcgacgtgg ccgaggtcat cgcgtcgtgg acgggcattc
     1501 ccgtctcgca gatgatggag accgaggccc agaagctgct gcacatggaa gagcggctcc
     1561 acgagcgcat tgttggccag gaacgggcga tcgccgccct gtccgatgcc attcgccgca
     1621 gccgttccgg cctgagcgac ccgcgccggc cgattggctc gttcatcttc ctgggcagtt
     1681 ctggcgtggg caagacggaa ttggccaaag cattagccga attcctgttt gacgacgaag
     1741 aggcgctgat ccgcgtggac atgagcgagt atcgcgagca gcacaccgtc agccggctct
     1801 tcggcgcgcc gccgggctac gtcggctatg agcagggcgg ccagttgacc gagcagatcc
     1861 gtcgccggcc ctattcggtg gtgttgtttg acgaaatcga aaaggcccac cccgacatct
     1921 ggaacgcgct gctgcaactg ctggacgacg gccgcctgac cgatggtcag ggccgcgtgg
     1981 tcaacttccg caacgcggtc atcgtcatga ccagcaacgt cggcacgtcg ttcgtcaagc
     2041 gctccggcgc gctgggtttc gccggcatgg ccgcggccga gcgcgaggac cacaagcgta
     2101 tcgaggaggc cctgaagcag accttccggc cggagtttat taaccggatc gatgaggtca
     2161 tcatcttcga gccgctgtct gaagtggacg tggttgagat tgttaagctc caggtcaagg
     2221 acgtgcaaat gcgcctggct gagcatggca atctgagcat cgaactgacg gagacggccc
     2281 agcgttggct ggccgcgcag ggctttgata aggacttcgg cgcgcgtccg cttaagcggg
     2341 ccatccaacg ctacgtcgaa agcccgctgt ccatgcgcct gctgcgcggc gagttcaaga
     2401 gcggcgaccg ggtgcgcatc gacgtggccg acgaccagtt ggactttatc ttgctggaga
     2461 gcggccctgt gccccagaag gccgtcgaag agatggccga gcacgcttag ggcgagcagc
     2521 gccacactcc tgtgatcctt gcggcccccg ccgaccggtt acccggccgg tgggggctgt
     2581 gtttgttggc gctcagtcct cccagcgctg ttgccgttcg cgcttgacgg tgctgtggcg
     2641 acgcttgtcg tcgaggcgcg cctggcgagc gcggcggctg gggcgggtgg gccggcgctc
     2701 ggcctgctcc gccagcgcgc cggccagcag gctttgcagg cgggcgatgg ccagctcgcg
     2761 gttctgccac tggctgcgcg agtcctgcac gtcgatgtgg agcacgccac gccgatccag
     2821 ccggttggcc agccggtcga gcaggcgcgc ccgcgttgcc tcgtcgaggc tgggggtgcg
     2881 ggccacgtcg aacagcagcg tcacgcgcgt agccgtcttg ttgacgtgct gcccgccggg
     2941 accgccgccg gtcgtgaagc gaaataccag ctcggccagg ggtagagata gcgcctcggt
     3001 gatggggatg agatcagttt ccatgatttc cgcgctgtgc cgtttaggac gttcacggcc
     3061 cttatgctac gattatcagc catgcccggc caagtgacaa cgcccgaccg cgcccacgtc
     3121 cacatcttct ttatggacgg tgttggcctg ggcgcggccg acccggccga gaaccctttc
     3181 gccgcggccc gcctgctaaa cctgaccgcg ttgctgggcg cggagtggtt cgtcggcgga
     3241 cgcggccctg ttgccgcggc gcgggcgacg ctcgtgccta ccgacgccac gctgggcctg
     3301 cccggtcgcc cgcagagcgc caccggccag gcgactattc tgaccgggcg caacgtgccc
     3361 gcgctggtgg gcgagcacta tggccccaag cccaacgccg ctgtggccaa catcgttcgt
     3421 cagggcacgc tgttcagcga agtcgccgcc gcgggcggcc gggccgcgct gctgaccccc
     3481 tatccggagc gctacttcga ggccatcaac agcggccggc ggttgctatc ggccgtgccc
     3541 ctggccgcgg ccagcgccgg gctgccgctg ctgggcgtcg atgacctgcg cgccgggcgg
     3601 gcggtcagcc ccgatttcac cggcgcgggc tggcgcgact tcctgggcta tgccgatgtg
     3661 gccgtcctgt cgccggagga cgccggccag cgcattgccg ccctggctgc ggcccacgac
     3721 ttcagcttct tcgagcactg gccgacggac caggttggcc accgcggctc gctggcggag
     3781 gccgtggccc acctggaaag catggacgcc gctctgggtg gcctgctgga tgcctgggac
     3841 gatgcccaca gcctgctcat cctgaccagc gaccacggca acctggaaga gaagagccac
     3901 cgccagcaca cgcgtaacgc cgtgccgacg atcctcgtcg gccgcgacca tgccgcccac
     3961 gccgcgacca tccacgatct gaccgacatc gccgctgtcg tccggcgcgt cctcggcctg
     4021 ccaacgccca actactagcc cccaggagaa cactatgccc attacgtccg agagtttccg
     4081 cgacgtcttg cgacactttc cgtccggcgt gaccgtcgtg accatccaat cgccggcctc
     4141 ggacgtcgtc cacggcctga ccgtatcggc gtttgcctcg gtgtcgccag agccgccgct
     4201 ggtgctggtg tcgatcgatc accggacgac ggcctatatg ctgctggagg cggccggagc
     4261 cagctttgcc gtcaacatcc tggcccatga tcagatggaa ctgtccaacc gctttgcctg
     4321 gctcaaggac gaggatcgct tccagatagg cgattggacg acagcggtga cgggcgcgcc
     4381 tattctgcgc gaggcggtgg cctggctcga ctgtaccgtc tactcgcgct tcgccgccgg
     4441 cacgcacacg atctacattg gcgaagtgca ggccagcggc gtgcctcgcc cggacgtcaa
     4501 gccgctgctc tactggaacc gcggctaccg gcagatgagc aagtaaggct gccggcgctc
     4561 tacgtctcgt cgctggggcc catctctaac tcctccaggc ccacggccat tagatagccg
     4621 cgcgcgcgct cggtcagggg atagcggttg acaagcttcc agaagcgctt gccgtggttg
     4681 cgctccacca ggtgggccag ttcgtggatg agcacatagt cgctcaccca ggccggcatc
     4741 gatgccaggc ggtggctaag gcggatagtg ccatcggagg gggtacaact gccgaagcgg
     4801 gtgttctggt tggtcacata gcggatggat tgccagcgca gacggccgtc gaagtacttg
     4861 cggtttagcg cctcggcccg tttctccagc gcttcgtcgg acagtggctc cgaccggccg
     4921 cggcgacgca tccgctggcg caagttgtcg atgatcgggg ccaggtcagc gtcgctcagc
     4981 ccttccgggg cgcggatgac cagcacaccg ccttccagcc gcgcgctgac cgtcttgcgg
     5041 cgttgggcac tgcggatgat ctggacgggc cagttttcct cggtggcttt gcccatagac
     5101 cctgttgacg ccgggttatt gatgccggcg aacccattct aacaccggcg cggcagggca
     5161 caaccgctta tctggccggg cggcggctgg cgtattcttc cggccggtca aggtcgatga
     5221 gcacggccgc gtcttcgacc tcgacggcca gcaagtcgct ggcgtggcgg gccagcagca
     5281 cgcgcggcgc ggcgccggcc ggcagagcca gcaactcgtc gaaatagcga cgatcgatga
     5341 tcaccggatt gccgcgccgg ccgtcgtaga tgggggcgac gaggccggcg ggcgtggccg
     5401 cgtaggcgcg tagcaggctg tcgagcgtgt ccgtcgccac cagcggctgg tcgccgagca
     5461 tgatgaggat cgcctcggcg ctggggggca gggcgcgcac agcggtctgg acggaggtag
     5521 catcccattc gcatagtcat gattgtgga
//
LOCUS       DAOSMQ010000188         5547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14788_length_5547_cov_3.885288, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000188 DAOSMQ010000000
VERSION     DAOSMQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14788_length_5547_cov_3.885288"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PK829_07550"
     CDS             <1..1028
                     /locus_tag="PK829_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOU41107.1"
                     /translation="RVERVPVPAIGPDEVLLRVLRANICGTDLRILHGGHRMYSPGTV
                     RIPGHEVVGEVAAVGARVDGYRPGDRLFVAPNMGRGDSRETISGNNNLDPQFDAFGIT
                     LDGAFAEYMRVPAAAVRGGNLMPVRPEVELAVAALIEPLACVWRGQNKIGVGAGDVVL
                     VMGAGPIGVLHILLARLRGATRVIVSEPAAGRREQALRLGADRAVDPLGEDLAAIVRA
                     QSDGRGADAIITAAPVRALQEQAVALAAVGGRINFFGGLSKDDALIQLDSNAVHYKEL
                     VVTGTTACSTYDCLRAAEIVNSGRLDLAPLITAEFPLAEAPAAFAAAGDGRNLRVTLI
                     PDAPRQS"
     gene            1043..2056
                     /locus_tag="PK829_07555"
     CDS             1043..2056
                     /locus_tag="PK829_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41108.1"
                     /translation="MSTGHIVTVLGPVVTLGIADAHNHLYIGRVPGAPDDAPALTDSE
                     GVARELIRFRAMGGGGIVDCQPGGCGRDGRVLRALSEQTGVAIVAATGFHRRRYYPPD
                     APLFALSAEAAASYFLGEIRGGLEETRGKDPSPQPSPGGRGSKTEPSPPGRGGENVYP
                     GVIKMAAEATLAESPAALFAAAAAVAAETGYAIEVHTERGAAVEALLAYFVDRGVAPE
                     RLVFCHVDKRPDVGLHRELAAAGVLLEYDTFYRPQYDPERGVWPLLAQMLAAGWGQRI
                     ALATDMADPALWAELGGGPGLPGIFTVIKPRLEQMGADAATVAGLLGGNICARLRLED
                     NDE"
     gene            2049..2633
                     /locus_tag="PK829_07560"
     CDS             2049..2633
                     /locus_tag="PK829_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU41109.1"
                     /translation="MSDTPYTFDVNAPDGVPGRYDNHIRRTLTSMRGQYLDTAAYEAM
                     LAEDDALLYEVYELQRPHVAGELLHGISIVHPGRVGDEYFMTKGHFHSVLATAEVYHC
                     LKGEGFMVMETPEGEWAVEPLRPGSVLYVPPCWAHRSINTGTSDLVTLFAYPGHAGHD
                     YGTIERQGFRKLIVARDGQPAIIDNPRWGQARDD"
     gene            2665..3633
                     /locus_tag="PK829_07565"
     CDS             2665..3633
                     /locus_tag="PK829_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOU41110.1"
                     /translation="MKPLNECHVLVTPTSYAQHDATLRTALEAAVGRVTYNETGRPLS
                     SAQVAALLPGVDGYIAGVDAVDAAALAAADALRVIARYGVGVDKVDLTAAAARGIVVT
                     NTPGANARSVAELTVALLLLLARPVLRAAAETRAGGWPRLPGLSLAGKTVGLIGFGAI
                     GRQTARLLGGFDCRLLAHDPVLNEATAAALGVRAAALDALLAESDFVSLHTLLSPQTR
                     GLVDAAFLARMKPGACLINTARGELVDEAALLAALTDGRLRGAALDVFAAEPPAADHP
                     LLALPNVIATPHMGAHTDGATTAMGRMALEDCLAVLRGEEPKYRVG"
     gene            3661..4209
                     /locus_tag="PK829_07570"
     CDS             3661..4209
                     /locus_tag="PK829_07570"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU41111.1"
                     /translation="MTDVMDPQDVWPAGETALVTIFRHHLWANGKLFDACAGLDEAQL
                     AATAAGTYGPIYDTLHHLVRAEQGYLFLLTGDSPVARMRPEDRPAMATLRENVRLFDE
                     ELMAVAAGVTPPAMAHSVEEDGQWSLPAGVVLAQVINHATEHRAHIMTILTQIGLQPP
                     ELDCWAFGDRYGTFTPNDDRAG"
     gene            4267..4926
                     /locus_tag="PK829_07575"
     CDS             4267..4926
                     /locus_tag="PK829_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOU41112.1"
                     /translation="MSDKESTLNRLRDLGLVAVIRGPAPELTVRMVEALVAGGVLGIE
                     ITYTTPNAAAVVHALDARFGEQILLGMGTLTRPEQAEEALAAGARYLVSPITDETLGR
                     AMVATGLPVMIGALTPTEVWRAHQLGSDVVKLFPGSAVGPDYVKALRGPFPNIRLMPT
                     GGVDEHNVGDWFRAGVFAVGAGSQLSPGQLAKEGRFDAIEATARRFAAAVAAARATAD
                     R"
     gene            5041..>5547
                     /locus_tag="PK829_07580"
     CDS             5041..>5547
                     /locus_tag="PK829_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOU41113.1"
                     /translation="MSHPYPILTQPQPTMYFIGVTTGKSSINKVFPLWMDVLNRPDVV
                     LRGIDHPMHDDPQRYRASVAQIKYDPLSLGALVTTHKIDLFTAARDLFDYADSYAQIT
                     GEVSSISKRDGRLEGHAKDPITAGLSLEAIVEPGYFGRTGAEVLCLGAGGSAVATLLY
                     LMNKPDPAD"
BASE COUNT          849 a         1870 c         1970 g          858 t
ORIGIN      
        1 ttcgcgttga gcgcgtgccc gtgccggcca ttgggccgga cgaggtgctg ttgcgcgtgc
       61 tgcgggccaa catctgtggc accgacctgc gcatcctcca cggcggccac cgcatgtact
      121 cgccgggcac ggtgcgcatc cccggccacg aggttgtggg cgaggtggcc gccgtgggcg
      181 cgcgcgtgga cggctaccgc ccgggcgacc gcctctttgt ggctcccaac atgggccgcg
      241 gcgacagccg cgagacgatc agcggcaaca acaacctcga cccacagttc gacgccttcg
      301 gcatcaccct cgacggggcc tttgccgagt acatgcgcgt gccggcggcg gccgtgcgcg
      361 gcggcaactt gatgcccgtg cggccggagg tggagctggc cgtggccgcg ctcattgagc
      421 cgctggcctg cgtctggcgg gggcaaaaca agatcggcgt cggcgcgggg gatgtcgtcc
      481 tggtcatggg cgcggggccg attggggtgc tccacattct cctggcccgg ctgcgcgggg
      541 cgacgcgcgt catcgtcagc gagccggccg ccgggcggcg cgagcaggcg ctgcggctgg
      601 gggccgaccg ggctgtggac ccgctgggtg aagacctggc cgccattgtc cgcgcgcaga
      661 gcgacggccg tggcgccgac gccatcatca ccgccgcgcc ggtgcgggcg ctgcaagagc
      721 aggccgtggc tctggccgcc gtcggcgggc ggatcaactt cttcggcggc ctgtcgaagg
      781 atgacgcgct catccaactg gacagcaacg ccgtccacta caaggaactc gtcgtcaccg
      841 gcacgacggc gtgcagcacc tacgactgcc tgcgcgcggc tgaaatcgtc aacagcggcc
      901 ggctcgacct ggctcccctg atcaccgccg agtttccgct ggcggaggcc ccggccgcct
      961 tcgccgccgc gggcgacggc cggaacctgc gcgtgacgct gatcccggac gcgccgcgcc
     1021 agtcatgact ggctagatgc ttatgtcaac aggtcatatc gtcactgttc ttggccccgt
     1081 ggttacgctg ggcattgccg acgcgcacaa ccacctctac atcggccgcg tccccggcgc
     1141 gcccgacgac gcgccagcgc tgaccgacag cgagggcgtg gcgcgcgagc taatccgctt
     1201 ccgggcgatg ggcggcggcg gcatcgttga ttgccagccg ggcggctgcg gccgcgacgg
     1261 gcgcgtgctg cgggcgctgt cagagcaaac gggtgtggct atcgtggccg caactggctt
     1321 tcaccgccgg cgctactatc cgcccgacgc gccactcttt gctttgagtg ctgaggcggc
     1381 ggccagctat tttctggggg agatacgtgg tgggttggag gagacgaggg gcaaagaccc
     1441 ctctccccaa ccctctcccg gagggagagg gagcaagact gaaccctcgc cccccgggcg
     1501 agggggcgag aacgtttatc ccggcgtgat caagatggcg gccgaggcca cattggccga
     1561 gtcgccggcg gcgctgttcg cggcggcggc cgccgtggct gccgagacgg gctacgccat
     1621 cgaggtgcac acagagcggg gcgcggccgt cgaggcgctg ctggcctatt tcgtcgatcg
     1681 cggcgtggcc ccggagcggc tggtgttttg ccacgtggac aagcggccag acgttgggct
     1741 gcaccgcgag ctggccgcgg ccggggtgct gctggaatat gacacctttt accggcccca
     1801 gtatgacccg gagcgcggcg tctggccgtt gctggcgcaa atgctggccg cgggctgggg
     1861 gcagcggatc gccctggcca ccgatatggc cgacccggcg ttgtgggcgg agcttggtgg
     1921 cgggccgggg ctgccgggca tcttcacagt catcaagccg cgtctggagc agatgggcgc
     1981 ggacgcggcc acggtcgcgg gcctgttggg gggcaacatc tgcgccagac tgcgcctgga
     2041 ggacaatgat gagtgataca ccttacacct ttgacgtcaa cgcgcccgac ggcgtgcccg
     2101 gccggtatga caaccacatc cggcggacac tgacgtcgat gcgcgggcaa tatctggaca
     2161 cggccgccta cgaggccatg ctggccgagg acgacgcgct cctctacgag gtctacgagt
     2221 tgcagcggcc ccacgtcgcc ggggagctgt tgcatggcat ctccatcgtc caccccggcc
     2281 gcgtcggtga tgaatacttc atgaccaaag gccactttca tagcgtcctg gccacggccg
     2341 aggtctatca ctgcctgaag ggcgagggct tcatggtcat ggagacgccg gaaggggagt
     2401 gggcggtgga gccgctgcgg ccgggcagcg tactctatgt gccgccgtgc tgggcgcacc
     2461 gttcgatcaa caccggcacg agcgatctgg tgaccctgtt cgcctatccg ggccacgccg
     2521 gccacgacta cggcacgatc gagcggcagg gcttccgcaa gctgatcgtg gcgcgcgacg
     2581 gccagccggc catcatcgac aacccgcgct ggggccaggc gcgcgacgat tagaacgacg
     2641 gccccgcgaa gccggagaga cgccatgaag ccactcaacg aatgtcacgt cctggtgacg
     2701 cctacctcct atgcccagca cgacgcgacg ctgcgcacgg cgctggaagc ggccgtcggc
     2761 cgcgtcacct acaacgagac gggccggccg ctgtcgtcgg cgcaggtggc cgcgctgctg
     2821 cccggcgtcg acggctacat cgccggcgtc gatgcggttg acgccgcagc gctggccgcg
     2881 gccgatgcgc tgcgcgtcat cgcccgttac ggcgtcggcg tcgacaaggt cgatctgacc
     2941 gccgcggcgg cgcggggcat cgtcgtcacc aacacgccgg gggccaacgc acgctcggtg
     3001 gccgagctga cggtggcgct gttgctactg ctggcccgtc cggtgttgcg ggcagcggca
     3061 gagacgcgcg ccggcggctg gccacgactg ccggggctgt cgctggcggg caagacagtt
     3121 ggcctgatcg gcttcggggc catcgggcgg cagacggcgc ggctgctggg cggcttcgac
     3181 tgccgcttgc tggcccacga tcccgtgctc aacgaggcca cggccgcggc gctgggcgtg
     3241 cgcgccgccg cgttggacgc gctactggcc gagagcgact tcgtgtcgct gcacacgctg
     3301 ttgtcgccgc agacgcgcgg gctggtcgat gccgcctttc tggcgcggat gaagcccggc
     3361 gcgtgcctga tcaacaccgc ccgtggcgaa ctggtggacg aggcggcgct gctggcggcg
     3421 ctgacggatg gccggctgcg cggcgcggcg ttggacgtgt tcgccgctga gccgccggcc
     3481 gccgatcacc cgctgctggc cctgcccaat gtcatcgcca ccccccacat gggcgcccac
     3541 accgacggcg cgacaacggc gatggggcgt atggccctgg aggattgcct ggccgtcctg
     3601 cgcggggagg agccaaagta tcgggttggg taagagcttc taaggataga gggagtgaca
     3661 atgactgacg tgatggatcc tcaagatgtt tggccggcgg gcgagacggc gctggtgacc
     3721 atattccggc atcatttgtg ggccaacggg aagttgttcg acgcctgcgc cgggctggat
     3781 gaggcgcaac tggcggcgac ggccgccggc acgtatggcc cgatctacga cacactgcac
     3841 cacctcgtgc gcgccgagca gggctatttg ttcctcctga cgggagattc gccggtggct
     3901 cgcatgcggc cggaagacag gcccgctatg gccaccctgc gcgaaaatgt ccgtctgttc
     3961 gatgaagagc tgatggccgt ggccgctggc gtcaccccgc cggcgatggc tcactcggtg
     4021 gaagaggacg gccagtggtc gctgccggcc ggcgtggtgt tggctcaggt gatcaaccac
     4081 gccaccgagc accgcgccca catcatgact atcctgaccc agatcggcct gcaaccgccc
     4141 gaactggact gctgggcgtt tggcgaccgc tatggcacgt ttacgcccaa cgatgatcgc
     4201 gcgggatagc gttccgcgcc gtccagatgc gggataccat cccgcgctac aagtagacga
     4261 ggcgatatga gcgacaaaga atcaacgttg aaccgactgc gcgacctcgg tttggtggcc
     4321 gtcattcgcg ggccagcgcc ggagctgacc gtgcgcatgg ttgaggcgct ggtggctggc
     4381 ggcgtgctgg gcatcgagat cacctacacc acgccgaacg ccgccgcggt cgtccacgcg
     4441 cttgacgccc gcttcggcga acagatcttg ctgggcatgg gcacgctgac gcggccggag
     4501 caggctgagg aagcgctggc cgccggagcg cgctacctgg tcagtcccat caccgacgag
     4561 acgctcggcc gggccatggt cgccaccggg ctgccggtca tgatcggcgc gctgacgccg
     4621 acggaggtct ggcgggccca ccagctcggc tcagacgtag tgaagctgtt ccccggctcg
     4681 gccgtcggcc ccgattatgt caaggcgctg cgcgggccgt tccccaacat ccggctgatg
     4741 cccaccggcg gcgttgacga gcacaacgtg ggcgactggt tccgggcggg cgtcttcgcc
     4801 gttggcgcgg gcagccaact gtcaccgggc cagctggcca aagaggggcg cttcgacgcg
     4861 atcgaggcca cggcgcggcg gtttgcggcg gccgtggcgg cggcgcgggc gaccgccgac
     4921 cgctgacggc cgaccgcgga cggccgaccg cggacgacag accactgatc caccgcccca
     4981 ctgacttact gacccactgg cttactggcc actactcact catcacaccg gaaaggcact
     5041 atgtcacacc catatccgat cctcacacag ccgcagccga cgatgtactt catcggcgtg
     5101 acgacgggca agtcgtcgat caacaaggtc tttccactgt ggatggacgt gctcaaccgg
     5161 ccggacgtcg ttctgcgcgg catcgaccac cccatgcacg acgacccgca gcgctaccgc
     5221 gccagcgtgg cccagatcaa gtacgatccg ctcagtctgg gcgcgctggt gacgacgcac
     5281 aagatcgacc tgttcaccgc tgcccgcgac ctgttcgact acgccgatag ctatgcccag
     5341 atcaccggcg aggtgtcgtc gatctccaag cgtgacggcc ggctggaggg gcatgccaag
     5401 gaccccatca ctgccggcct gagcctggaa gcgatcgttg agccgggcta cttcggccgc
     5461 accggcgcgg aggtgctctg cctgggggcg ggtgggtcgg ccgtggctac gctgctatac
     5521 ctgatgaaca agcccgaccc ggccgac
//
LOCUS       DAOSMQ010000189         5526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14845_length_5526_cov_5.541035, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000189 DAOSMQ010000000
VERSION     DAOSMQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14845_length_5526_cov_5.541035"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1673
                     /locus_tag="PK829_07585"
     CDS             66..1673
                     /locus_tag="PK829_07585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU41114.1"
                     /translation="MIDLLLTNARVYTVDPAQPWAEAVACRNGRIIAAGRAADLDALA
                     GPTTRRIDCGGRLLLPGLVDAHVHTLATAVRRRQVSLFGLSDLEAVRARLAAAAAGVA
                     PGEWLLGWGWDSNRWDEQPTAALLDAIVPDHPVALARMDMHTWWVNSRALALAGVSAA
                     TPDPADSHIHRDASGRPTGLFSEWNAIALIERVVPQPDAGALAEWLAETVAEANRLGL
                     TGIHDQRVEREGAESLRLFQALRRQGRLSLRVHANIAADYVAEAARLGLQAGFGDDNL
                     WLGHVKAFADGAMGSRTAAMLTAYEGEPDNTGIVVTPADRLWPTLVAAGEAGFPMSVH
                     AIGDRAVREVLDVMTEWATTRGATTSLPMPQRIEHVQLIHPDDLARLAQSGIVASVQP
                     VHLQSDWRTADRVWGGRARLAYAFRSLLDRGTALAFGSDAPVAPLNPLLGIHAAVTRQ
                     DEGGAPAGGWYPAERLTVAEAIAGYTLGPAQLSGKADRFGSITPGKYADLVVLSDNLF
                     EIDPAAIAAASVWLTVFDGRVVYGAEE"
     gene            1746..4148
                     /locus_tag="PK829_07590"
     CDS             1746..4148
                     /locus_tag="PK829_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOU41115.1"
                     /translation="MIIGMDFGTTNSGMAVYDGRAVEVLPLDPAAPNPRVARTALYVT
                     NEQAVTLGRAAIDRYYEHNLNRPVKMQRIWVGELEVIADRVYYVTDVYTWADALSPGR
                     LFLSIKTGLRDVNYPGTVVGQFYYSLETLIALYLTVAKARAEHLLGRELRQVVLGRPV
                     HWANDAAGDRVAEARLLHAAFLAGYETVYLQYEPVAAAYSYALGLSRPENALVFDFGG
                     GTLDITVMRLGEGRPRVLATGGIPVAGDVFDQKLVRAKLPRHFGEGSLYGPRHKALAT
                     PQWIYDSFNDWQTILQLNSPQNRQILRDIAQTAQHKHQIEALLALISSNYGLKMFDVV
                     EAAKRTLSDKRGAEIYLDGPGFNVREFVTRTEFEDVIRAETRAIEHHLTETVQRSGVR
                     PEQIDTVIRTGGSALIPAFYDMLGRHFGEARVRSIDAFSSVTAGLGVLAHHLERGEVE
                     LPAHTTADFANLPEATGGRLKVRPVNLELIQRRIVLEEQGSGGAGEQGSALVLLGKPV
                     SQGEWTRAAVVVAEEPAAAADGAASLVELGWRDGQVDAGVRLPPDDQLLLVTSRYRFL
                     LTSARQLRDLQGIGQNVENLYRLASREAVVAVVDWTAVREAERLLFVTSTGFARAYPL
                     ETLRPSIEAPVPFSFDSPLPGVPVLVAGIARRQEMVIVTASGRGLRWPLARLPLAGAQ
                     AINCGQEASFDRVAAALAAEPDGEAVLLLADGYARRLPVAAVAEAPKANTRGKALVAR
                     RAAVVGLAPVATLTVATNGRLLAADTRALPLETGTRAARLVRLGNDEAVAAVWASAPA
                     TP"
     gene            complement(4338..5105)
                     /locus_tag="PK829_07595"
     CDS             complement(4338..5105)
                     /locus_tag="PK829_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU41116.1"
                     /translation="MNTHDFPILEHDPSPDALIEPSRLLAPIDIPRHCVLCFFQEVID
                     DLGARGLLRVVHTLRSEIGPNPVYVLDVDGRSLALVHPGLGAPLAGAFMDELIALGCR
                     AFVACGGAGVLAPELVVGHIVVPNAAVRDEGTSYHYLPPSREVAASVSAVAAIEATLQ
                     RHGAPYVVGKTWTTDAFYRETAARVARRRAEGCLTVEMEAAAFFAIGQFRGVTVGQIL
                     YSADDLSGEEWDGRAWQGRGETRAGLFWLAAEACLRL"
     gene            complement(5152..5484)
                     /locus_tag="PK829_07600"
     CDS             complement(5152..5484)
                     /locus_tag="PK829_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HOU41117.1"
                     /translation="MILHIAAADEWDAARALGEYRADTLLAEGFIHCSTPAQVLVPAN
                     ERFHGRTDLLLLVIDPARLEAALVYEDCYETGQVFPHIYGPLNLNAVLRAVPFPPSAD
                     GSFALPPL"
BASE COUNT          820 a         1942 c         1993 g          771 t
ORIGIN      
        1 tggaccagcc gccggcccga ccgcgccgtc atgcgccgcg ccgccgaggc cgtggaggcg
       61 caaccatgat cgacctgctc ctgaccaacg cccgcgtcta caccgtcgat ccggctcaac
      121 cgtgggccga ggctgtggcc tgccgcaatg gccgcatcat cgccgccggc cgggcggccg
      181 acctcgacgc cctggctggc ccgacgacgc gccgcatcga ctgtggcggc cggctgctgc
      241 tgcccggcct cgtcgatgcc cacgtccaca ccctggccac cgccgtgcgc cgccggcagg
      301 tcagcctctt tggcctgagc gacctcgagg ccgtgcgcgc ccggctggcc gcggcggccg
      361 ccggcgtcgc ccccggcgag tggctgctcg gctggggctg ggacagcaac cgctgggacg
      421 aacaaccgac ggcggccctg ctcgatgcca tcgtgcccga ccaccccgtg gccctggccc
      481 gcatggacat gcacacctgg tgggtgaaca gccgcgccct ggccctggcc ggcgtcagcg
      541 cggccacgcc cgacccggcc gacagccaca tccaccgtga tgccagtggg cggccgacgg
      601 gcctgttcag cgagtggaat gccatcgccc tcatcgagcg cgtcgtcccc caaccggacg
      661 cgggcgcgct ggccgagtgg ctggccgaga ccgtggccga ggccaaccgg ctgggcctga
      721 ccggcatcca cgaccagcgc gtggagcgcg agggggcgga gagcctgcgc ctcttccagg
      781 ccttgcggcg gcaggggcgg ctgtcgttgc gcgtccacgc caatatcgcc gccgactacg
      841 tggccgaggc ggcccggctg ggcctgcaag cagggtttgg cgacgacaac ctctggctgg
      901 ggcacgtcaa ggcatttgcc gacggggcga tggggtcgcg cacggcggcg atgctgaccg
      961 cctatgaggg cgagccggac aacaccggca tcgtcgtcac cccggccgac aggctgtggc
     1021 cgacgctcgt cgccgcgggc gaggccggct tccccatgtc ggtgcatgcc atcggcgacc
     1081 gggccgtgcg cgaggtgctg gacgtgatga ccgagtgggc gacgacgcgc ggggcgacca
     1141 cttctctgcc catgccccag cgcatcgagc acgtccagct catccacccc gacgacctgg
     1201 cccggctggc gcagagcggc attgtcgcct ccgtccagcc ggtgcatttg cagagcgatt
     1261 ggcgcacggc cgaccgcgtc tggggcgggc gggcgcgcct ggcctatgcc ttccggtcgc
     1321 tgctcgaccg cggcacggcg ctggccttcg gctccgacgc gccggtggcc ccgttgaacc
     1381 cactgctggg catccacgcc gccgtgacgc ggcaggacga gggcggcgcg ccggccggcg
     1441 gctggtatcc ggccgagcgg ctgacggtgg ccgaggccat cgcgggctac accctggggc
     1501 cggcgcaact gtccggcaag gccgaccgct ttggctcgat cacccccggc aagtacgccg
     1561 atctcgtcgt gttgtcggac aacctgtttg agatcgaccc ggcggccata gccgcggcgt
     1621 cggtctggct gacggtgttt gatgggcggg tggtgtatgg ggccgaagaa tagcacgctg
     1681 attttcgctg agaagagcgc tgattcagcg tcctattctg cgccggcgcg ggctataatc
     1741 catccatgat catcggcatg gacttcggca cgacgaacag cggcatggcc gtctacgacg
     1801 gccgcgctgt tgaggtgctg ccgctcgacc ccgccgcgcc caacccgcgc gtggcccgca
     1861 cggccctcta tgtcaccaac gagcaggccg tcaccctcgg ccgggcggcc atcgaccgct
     1921 attacgagca caacctcaac cggccggtca agatgcagcg catctgggtc ggcgagctgg
     1981 aggtcatcgc cgaccgcgtc tactacgtca ccgatgtcta tacgtgggcc gacgccctct
     2041 cgcccggccg cctcttcctg tccatcaaga ccggcctgcg cgatgtcaac taccccggca
     2101 cggtcgtcgg ccagttctac tactcgctgg aaacgctcat cgccctttac ctgaccgtcg
     2161 ccaaggcgcg ggcggagcat ctgttggggc gcgaactgcg ccaggtggtg ctcggccggc
     2221 ccgtccactg ggccaacgac gccgccggcg accgcgtggc cgaggcgcgg ctgctgcacg
     2281 ccgccttcct ggccggctac gagaccgtct atctgcaata cgagccggtg gccgcggcct
     2341 atagctacgc cctcggcctg agccggccgg agaacgcgct ggtgttcgac ttcggcggcg
     2401 gcacgctcga catcaccgtc atgcgcctgg gcgagggccg gccgcgcgtc ctggccaccg
     2461 gcggcatccc cgtggccggc gacgtgttcg accagaagct cgtccgcgcc aagctgccgc
     2521 gccacttcgg cgagggcagc ctctatggcc cgcgccacaa ggcgctggca acgccgcaat
     2581 ggatctacga cagcttcaac gactggcaga ccatcctgca actcaactca ccccagaacc
     2641 gccaaatcct gcgcgacatc gcccagacgg cgcagcacaa acaccagatc gaagccctgc
     2701 tggccctcat cagcagcaac tacggcctga agatgttcga cgtcgtcgag gcggccaagc
     2761 gcaccctgtc ggacaagcgc ggcgcggaaa tctacctcga cggccccggc ttcaacgtgc
     2821 gcgagttcgt cacgcgcacg gagttcgagg acgtcatccg cgccgagacg cgggccattg
     2881 agcaccacct gaccgagacg gtgcagcggt cgggcgtgcg gccggagcaa atcgacacgg
     2941 tcattcgcac cggcggctcg gcgctcatcc ccgcgttcta cgacatgctc ggccggcatt
     3001 tcggcgaggc ccgcgtgcgc agcatcgacg ccttcagcag cgtcaccgcc gggctgggcg
     3061 tgctggccca ccatctggag cgcggcgagg tcgaactgcc ggcccacacg acggccgatt
     3121 tcgccaacct gccggaagcg acgggtgggc ggctgaaggt gcggccggtc aatctggagc
     3181 taatccagcg gcggatcgtg ttggaggagc aggggagcgg gggcgcaggg gagcagggca
     3241 gtgccctggt gttgctcggc aagcccgtta gtcaggggga gtggacgcgg gcggccgtgg
     3301 tggtggccga ggagccggcc gcggcggccg acggcgcggc ctcgctggtg gagctaggct
     3361 ggcgcgacgg gcaggtggac gcgggcgtgc gcctgccgcc ggacgaccag ttgctgctgg
     3421 tgaccagccg ttaccgcttc ctgctcacct cggcgcggca actgcgcgac ttgcagggca
     3481 tcggccagaa cgtcgagaac ctctaccggc tggcctcgcg cgaggctgtc gtcgccgtgg
     3541 tcgattggac ggccgtgcgc gaggcggagc ggctgctgtt cgtcacctcc accggcttcg
     3601 cccgcgccta cccgctggag acactacggc cgtcgatcga agcgccggtg ccgttcagct
     3661 tcgacagccc gctgccgggc gtgcccgtgc tggtggccgg catcgcccgg cggcaggaga
     3721 tggtcatcgt cacggcgagc ggccgggggc tgcgctggcc attggcgcgg ctgccgctgg
     3781 ccggggcgca ggcgatcaac tgtggccagg aggcatcgtt cgaccgggtg gccgcggcgc
     3841 tggcggccga gccggatggc gaggcggtgc tgctgctggc cgacggctac gcccgccgcc
     3901 tgcccgtggc cgccgtggcg gaagcgccga aggccaacac gcgcggcaag gccctggtgg
     3961 cgcggcgggc ggccgtcgtg ggtctggccc cggtggcgac gctgacagtg gccaccaacg
     4021 ggcgcttgtt agccgctgac acccgagcgc tgccgctgga gaccgggaca agggcagcgc
     4081 ggctggtcag gctggggaat gacgaggcag tcgccgcagt ctgggccagc gccccggcga
     4141 cgccctagcc cggcggtgtg gtcggcctac ggggccggcg accaccccac gggcgtgcta
     4201 tctgtcgcgc tggcccagac tgcggcaaca tcgttgaagc tcaggagcgc gaaatcaccc
     4261 agaagaagga catcctgccg cggatgaaca gatagtcccc ccgcggcgtc gggtgcgtgc
     4321 cggccgggca accgcgctca gaggcgcaag cacgcctcgg ccgccagcca gaacagcccg
     4381 gcgcgcgtct cgccccgccc ctgccaggcc cggccgtccc actcctcgcc gctgaggtcg
     4441 tcggcgctat agagaatctg ccccaccgtc acgccgcgga actggccgat ggcgaagaac
     4501 gccgccgcct ccatctccac cgtcagacag ccctccgccc gccgccgggc cacgcgcgcc
     4561 gccgtctcgc ggtagaaggc gtcggtcgtc cacgtcttgc cgacgacgta gggcgcgccg
     4621 tggcgttgca gggtggcctc gatggcggcc acggccgaga ccgaggcggc cacctcgcgc
     4681 gacggcggca ggtagtggta ggacgtgccc tcgtcgcgca cggcggcgtt tggcacaacg
     4741 atgtggccga cgacaagctc aggggccagc acgcccgcgc caccacaggc gacaaacgcc
     4801 cggcagccga gggcgatcag ttcgtccatg aacgctcccg ccagcggcgc gcccaggccg
     4861 ggatgcacca gggccagcga gcggccgtct acgtccagca cgtagaccgg gttcggcccg
     4921 atctcgctgc gcagggtgtg gacgacccgc agcaggccgc gcgcgcccag gtcgtcgatg
     4981 acctcctgga agaagcacag cacacagtgg cgcgggatgt cgatgggcgc gagcaggcgc
     5041 gacggttcga tgagcgcgtc ggggctgggg tcgtgctcca gaatggggaa atcgtgggtg
     5101 ttcatggcgt caggtgaagg gcgttggccg gacgtggcgc tggccgccgg gctagagcgg
     5161 cggcagggcg aatgagccgt cggccgacgg cgggaagggc acagcgcgca ggacggcgtt
     5221 gaggttcagc gggccgtaga tgtggggaaa gacctggccc gtctcgtagc agtcttcata
     5281 gaccagagcg gcttccagcc gtgccgggtc gatgaccagc agaagcaaat cggtgcggcc
     5341 gtggaagcgt tcgttggcgg gcacaagcac ctgggcggga gtggaacagt ggatgaagcc
     5401 ttcggcaagc agcgtatcgg cgcggtattc gccgagcgcg cgggcggcgt cccattcatc
     5461 ggccgcggcg atgtggagga tcatggactg gtagtatgtg cctgcgtggg gaaatgtgca
     5521 agttgt
//
LOCUS       DAOSMQ010000190         5517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14868_length_5517_cov_4.153790, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000190 DAOSMQ010000000
VERSION     DAOSMQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14868_length_5517_cov_4.153790"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PK829_07605"
     CDS             <1..1287
                     /locus_tag="PK829_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41118.1"
                     /translation="WRVALGGAAVGLLWGLWAMAWWLPENTADPTLLAQGARLGQWPV
                     PAFALHAFTFTALLAVAHWLLGRGGWQKAFRPSLVEGVFVAGGLLVFFAALVLPAYPW
                     APLRLLVLVGVTLLGLYVNKRRERPGSALSDLAGPAQPRALVALVAMPTAAVAVYAAG
                     AALEPSAAVIRAITAYGLVLGTAVLGWLAFVAALLLTLWPRLPSPPTTLQSRHDPHPE
                     RDTMTATLQDAIAAVRAGETGRAQMLTAEVIRDNPDDANAWYFLSQLVDSDARRAVYL
                     HKAVALDPDNSRARAEFDALPAAAVASLSGAAAPIEAGGVEMEPVEIELVEADDTALT
                     DAGLPAEALAESDATAAETAPEWLRPLSPEPAVAYESASAPVVAAVPPAAAQPRKTPP
                     PPPARQRGNGALTALFVILLLLTLAVLAVLAYLLLL"
     gene            complement(1290..2366)
                     /locus_tag="PK829_07610"
     CDS             complement(1290..2366)
                     /locus_tag="PK829_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41119.1"
                     /translation="MSISRWFGLAALMALGAALLLLGGCSAGAALPPVLFLRVDDAGR
                     AQLYRQDASGGAARRLSAHPAGDVLDFAAAPDGSHVVYSVLDGSEGALRLVDADGGGD
                     RELLACPAAECSAPVWSPDGKRLIYEHRPLSASGVLGSPRLMWLDPASGETIPLVATE
                     VIGYGARFSPDGAWLSYVSPEDEGVVLVPLAGGAQRLLSSRVGRPAAFSPDASQIVIS
                     DITLVAEAAQESSRVYLYRVSLAEEGRERLSPAQPVDDSAPAWSPDGQWIAFGRAPAD
                     TAAGRQLWLMRSDGSDARALTAEPAIFHGPPTWSPDGRALLYQRYDLNDPAATPQVWS
                     LRPSDGAQTLIAPGGYLPAWLPGG"
     gene            complement(2418..2813)
                     /locus_tag="PK829_07615"
     CDS             complement(2418..2813)
                     /locus_tag="PK829_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU41120.1"
                     /translation="MTATVDIFKFEKNVETFDAGQAIFSAGDEGHCMYVVQAGEVDVF
                     VNGWLIETVGPGGIFGEMALIDRSPRSATLIAHTDCRLVPIDETRFMQHVHRTPFFAL
                     QVMRIMTARLRRRMTTTVDDQEVVDDQEV"
     gene            complement(2810..3508)
                     /gene="phoU"
                     /locus_tag="PK829_07620"
     CDS             complement(2810..3508)
                     /gene="phoU"
                     /locus_tag="PK829_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOU41121.1"
                     /translation="MRQSFDRELQRLQAEIIRMGSEVEEHLTRVTTAFIERDTYTASQ
                     MIAADASINERRIQIGLDALTLIARQQPLAGDLRLIGAVMEIVGELERIHDYIKGIGK
                     ISLNIGATPILPTFVAHMPEMAETAREMLHLSLKAFVDHDKELAQRVPAMDDVVDALF
                     NRLYADILTYAANQPRQIDHANQLEWALHNLERSADRTINICEWVVYMVTGVYHEMSM
                     GEYEAPPQGRARHE"
     gene            complement(3581..4387)
                     /gene="pstB"
                     /locus_tag="PK829_07625"
     CDS             complement(3581..4387)
                     /gene="pstB"
                     /locus_tag="PK829_07625"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOU41122.1"
                     /translation="MTIPASDPSSGGPYAIEAHDLNIYYGSFRAVKDIDLRFSTHKIT
                     ALIGPSGCGKSTVLRAFNRMNDLIPISRTEGTVLFHGKNIYDADVDPVQVRRRVGMVF
                     QKPNPFPKTIYENVAWGARINGFKGSKADMDALVEESLRDAAVWDDVKDKLFQSGLSL
                     SGGQQQRLCIARAIATRPELILMDEPASALDPISTLRIEELIQDLKKNYTILIVTHNM
                     QQAARASDYTAFFNMDTDRAGYLVEYGQTDQVFTSPRNEMTEQYITGRFG"
     gene            complement(4406..>5517)
                     /gene="pstA"
                     /locus_tag="PK829_07630"
     CDS             complement(4406..>5517)
                     /gene="pstA"
                     /locus_tag="PK829_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOU41123.1"
                     /translation="AAFTAGRGHVGLAAGGEGGLGVEAEQRFYADRFVCDTEAAFTAR
                     CQTDAAPTGCVLEPRNQGSLVTLIQADVVNPVVQQAWFLAESLFHREEVMTEARAAYP
                     DADIHFRRWLTLDFLTRPQSSQPELAGVRTAILGSLWVVSIGILVGFPVGVGAAIYLE
                     EYADKRRRLNRFIQTNINNLAGVPSIIYGLLGLTVFVRLLEPITSGAVFGVGDATTAN
                     GRTVLSAGLTLALLILPVIIISAQEAIKAVPSSLREASLGLGATKWQTIWNHVVPNAI
                     GGIMTGVILAMSRIIGETAPLVVVGASTFITADPTSPFSKFTTLPTQIFQWTSRPQQT
                     FLLIAGAAIIVLIVLLFILNGFAIALRNRYAGRIG"
BASE COUNT          920 a         1763 c         1899 g          935 t
ORIGIN      
        1 tggcgggtgg cgttgggcgg cgcggctgtc ggcctgctgt gggggctgtg ggccatggcc
       61 tggtggctgc cagagaacac cgccgaccca acgctgctgg cccagggggc gcggctggga
      121 caatggcccg tgcccgcgtt tgccctccat gcctttacct tcaccgccct gctggccgtg
      181 gcccactggc tgctcggccg cggcggctgg caaaaggctt tccggccgtc tttggtcgag
      241 ggtgtgttcg ttgccggcgg tctgctcgtc tttttcgccg cgctggtgct gccggcctac
      301 ccgtgggcgc cgctgcggct gctggtgctg gttggtgtta cccttctagg tctttatgtc
      361 aataagcgcc gcgagcggcc gggcagcgct ctatccgacc tggccgggcc ggcgcagccg
      421 cgggcgctgg tggcgctggt agccatgccc actgcggccg tggctgtcta tgccgccggc
      481 gcggcgctgg agccatcggc cgcggtcatc cgggccatca ccgcctatgg gttggtgctg
      541 ggcacggccg tgctcggctg gctggccttc gtcgccgccc tgctgctaac tctctggccc
      601 cgcttgccat cccccccaac gacgctacaa tcgcgccatg atccgcaccc agagagggac
      661 accatgaccg ccaccctgca agacgccatc gctgccgtcc gcgccggcga aaccgggcgc
      721 gcccagatgc tgaccgccga agtcattcgc gacaaccccg acgacgccaa cgcctggtat
      781 tttctcagcc agctcgtcga ttcggacgcg cgccgtgccg tctatctgca caaggccgtg
      841 gcgctggacc cggacaacag ccgtgcccgc gccgagttcg acgccttgcc ggccgcggcg
      901 gtggcttcgc ttagcggggc ggccgcgcca attgaggccg gcggcgtcga gatggagcct
      961 gtcgagatag agcttgtcga ggcggatgat accgcgctaa cggacgctgg cctgcccgca
     1021 gaagccctcg ccgagagcga cgccacggcc gccgaaaccg ctccggagtg gctgcgcccg
     1081 ctcagtccag agccggcggt cgcctacgag agcgcgtccg cgccggtcgt cgcggctgtg
     1141 ccacccgccg cggcgcagcc gcgcaaaaca ccgccgccac cgcctgcccg ccagcggggc
     1201 aatggcgcgc tgacggcctt gttcgtcatt ctgttgctgc tgacgctggc cgtgctggcc
     1261 gtgctggcat atctgctgct gctatagcct tagccgcccg gcagccaggc aggcagatag
     1321 ccgccggggg cgataagcgt ctgcgcgccg tccgaggggc gcaggctcca gacctggggc
     1381 gtggcagcgg gatcgttgag gtcatagcgc tggtagagca gcgcgcggcc gtcgggcgac
     1441 caggtcggcg ggccgtggaa gatggccggt tcagcggtca gagcgcgggc gtcgctaccg
     1501 tcagagcgca tcagccatag ctggcggccg gcggccgtgt cggccggcgc gcggccaaag
     1561 gcaatccact gcccatcggg cgaccaggcc ggggcgctat cgtccaccgg ctgggcgggg
     1621 ctgagccgct cgcgcccttc ctctgccaga cttacccgat acaggtaaac ccgcgacgat
     1681 tcctgggccg cctccgctac cagagtgatg tcgctgatga cgatctggct ggcgtccggg
     1741 ctgaaggctg cggggcggcc gacgcggctg ctgagcaagc gctgcgcgcc gccagccagt
     1801 ggaacgagca cgacaccctc atcttccggc gaaacgtagc tgagccaagc tccatcgggc
     1861 gagaagcgcg ccccgtagcc aatgacctcc gtcgccacaa gcggaatggt ttctccgctg
     1921 gccgggtcga gccacatcag gcgcggcgaa ccgagcaccc cgctggcgct cagcggccgg
     1981 tgctcataga tcagccgctt cccatcaggc gaccagacag gggcgctgca ctcggccgcc
     2041 gggcaggcca gtagctcgcg gtcgccgccg ccgtcagcat caaccagccg caacgcccct
     2101 tcggagccgt cgaggacgct atagacgaca tggctgccgt cgggcgcggc ggcgaaatcg
     2161 agcacgtcgc cagccgggtg tgcgctcagg cggcgggcgg cgccgccact ggcgtcctgg
     2221 cggtagagtt gggcgcgacc ggcgtcgtcg acgcgcagga aaaggacggg cggcagcgcc
     2281 gccccggccg aacagccgcc caacaggagc aaagccgccc ccagggccat cagagcggcg
     2341 agcccgaacc agcgcgatat ggacatagaa cagtcggtgt ggtgaaggcc gcgaatgcca
     2401 accagcgcgg ccggcgctca gacctcctgg tcgtccacga cctcctggtc gtccactgtg
     2461 gtcgtcatgc gccggcggag gcgcgccgtc atgatgcgca tgacctggag ggcgaagaaa
     2521 ggcgtgcggt ggacgtgctg catgaatctg gtctcgtcga tgggcacgag ccggcagtct
     2581 gtgtgcgcta tcagcgtggc gctgcgcggc gacctgtcaa tgagggccat ctcgccgaag
     2641 atgccgccgg ggccgacggt ctcgatgagc cagccgttga cgaagacatc gacttcgccg
     2701 gcctgaacga cgtacatgca gtgaccctcg tcgcccgcgc tgaagatagc ctgaccggcg
     2761 tcgaaggtct caacgttctt ctcgaactta aagatgtcaa ctgtcgcggt cattcgtgcc
     2821 tcgctcgccc ctggggcggc gcttcgtatt cgcccatact catctcgtgg taaacgccgg
     2881 taaccatgta gacgacccac tcgcagatgt tgatggtgcg gtcggcggaa cgctccaggt
     2941 tgtggagggc ccactccagt tggttggcgt ggtcgatctg ccgcggctga ttggcggcat
     3001 aggtcagaat gtcggcatag agccggttga acagcgcgtc aacgacatcg tccattgccg
     3061 gcacacgctg ggccagttct ttgtcgtggt cgacaaacgc cttgagcgac aagtgcagca
     3121 tctcacgcgc cgtctcggcc atttcgggca tgtgggcgac aaaggttggc agaatcggcg
     3181 tcgccccgat gttgaggctg atcttgccga tgcccttaat gtagtcgtgg atgcgctcca
     3241 actcgccgac gatctccatc accgcgccta tgagacgcag gtcgccggcc agcggctgct
     3301 gcctggcaat cagcgtcagg gcgtcgaggc cgatttggat gcgacgctca ttgatggacg
     3361 catcggcggc gatcatctga ctggccgtgt aggtgtcgcg ctcgatgaac gcggttgtga
     3421 cgcgagtgag gtgctcctcg acctcactgc ccatgcggat gatctcggcc tgcagccgct
     3481 gcaattcgcg gtcgaaggat tgtcgcatgt agtcgcctcc tgggcggtcg cctcctgggc
     3541 ggtcgtttcc tgagcggtcg tttcctgagt gcaatggcgc ttagccgaag cggccagtaa
     3601 tgtactgctc ggtcatctcg ttcctggggc tggtgaagac ctggtcggtc tggccgtact
     3661 cgaccaaata gccggcgcgg tcggtgtcca tgttgaagaa ggcggtgtag tcggacgcgc
     3721 gggccgcctg ctgcatgtta tgggtgacga tcaggatggt gtagttcttc ttgaggtcct
     3781 ggatcaattc ctcaatacgc aaggtcgaga tggggtcgag agcggaggcc ggctcgtcca
     3841 tcaggatcaa ctccggccgc gtggcgatgg cgcgagcgat gcacagccgc tgctgctgcc
     3901 cgcccgatag cgacaggcca ctctgaaaaa gcttgtcttt cacatcgtcc cagacagccg
     3961 catcgcgcaa cgactcctcc accagggcgt ccatgtcggc cttgctgccc ttaaagccgt
     4021 tgatgcgcgc gccccaggcg acgttttcat agatcgtctt ggggaacggg ttcggcttct
     4081 ggaagaccat gcccacgcgc cggcgaacct gcacggggtc cacgtcggcg tcgtagatgt
     4141 tcttgccgtg gaagagaacc gtgccctcgg tgcgcgaaat ggggatgaga tcattcatgc
     4201 ggttgaaggc gcgcaggacg gtgctcttgc cacagccgga agggccaatg agggccgtga
     4261 tcttgtgcgt cgagaagcgc aggtcaatgt ccttgaccgc gcgaaagcta ccgtaataga
     4321 tattcaggtc gtgggcctcg atagcgtagg ggccaccgga agaggggtca ctggctggaa
     4381 tggtcataaa aggctcccac ggggcctagc caattcgccc ggcatagcgg ttacgcaggg
     4441 cgatagcaaa gccgttgaga ataaagagca ggacgatcag gacgatgatc gccgcgccgg
     4501 caatgagcag gaaagtctgc tgcggccggg acgtccactg gaagatttgc gtcggcagcg
     4561 tcgtgaactt ggagaacggg ctggtcgggt cggcggtgat gaaggttgac gcgccgacga
     4621 cgaccagcgg ggccgtctcg ccaatgatgc gcgacatggc caggatgacg ccggtcatga
     4681 tgccgccgat ggcgttgggg acgacgtggt tccagatcgt ctgccactta gtggccccca
     4741 ggccgaggct ggcctcgcgc aacgaactgg gcacggcctt gatagcctcc tgcgcgctga
     4801 tgatgatgac cggcaggatg agcagggcca gtgtcagacc ggccgaaagc accgtgcggc
     4861 cgttggccgt ggtggcatcg cccacgccga aaaccgcgcc gctggtgatc ggttccagca
     4921 ggcggacgaa gacagtcaga ccaagcaggc catagatgat cgacggcacg ccagccaggt
     4981 tgttgatgtt ggtctggatg aaacggttaa ggcggcggcg tttgtcggca tactcctcca
     5041 gatagatagc cgcgccgacg ccgacgggga agccgacgag gatgccaatg gacacaaccc
     5101 atagcgaacc gaggatggcg gtgcgcacgc cggccagttc cggctggcta gactgaggcc
     5161 gggtcagaaa gtccagcgtc agccaacgcc ggaaatggat gtccgcgtcg gggtaggccg
     5221 cccgggcctc ggtcatgact tcctcacggt ggaaaagcga ctcagccaga aaccaggcct
     5281 gctggacaac gggattgacg acatccgctt gaatcagagt gaccaggctg ccctggttgc
     5341 gcggctccag cacacagccg gtcggggccg cgtcggtctg gcagcgggcg gtgaaggcgg
     5401 cctcggtgtc gcagacgaag cggtcagcgt aaaagcgctg ctcggcctcg acgccgaggc
     5461 cgccttcacc gcccgctgcc agaccgacat ggccccgacc ggctgtgaag gcggcct
//
LOCUS       DAOSMQ010000191         5513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14884_length_5513_cov_4.247527, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000191 DAOSMQ010000000
VERSION     DAOSMQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14884_length_5513_cov_4.247527"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PK829_07635"
     CDS             complement(<1..1127)
                     /locus_tag="PK829_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOU41124.1"
                     /translation="MPKIAFIGAGSTVFAKNLLGDILSFPELSRATISLHDIDAERLR
                     TSERVARRVAASLAAAPVIEATADRRRALDGADYVITMFQIGGYRPATVIDFDIPRRY
                     GLRQTIADTLGIGGIMRGLRTVPVLLDMAGEMEALCPNATLLNYVNPMAINCWALSRA
                     TSIRTIGLCHSVQGTAEQLARDIGVPVEEINYLCAGINHMAFYLRFERRTAAGTEDLY
                     PRIRRVLAEGRVPDWNRVRYDLFGRFGYFVTESSEHLSEYVPWYIKRDRPDLIDRYNI
                     PLDEYITRCENQIAAWDAIRQLLEDESITLPVERTHEYGAYIIHSLETGQPRVVYGNV
                     ANDHLIDNLPQGACVEVPCLVDRNGIQPTRIGALPPHLAAL"
     gene            complement(1361..2263)
                     /locus_tag="PK829_07640"
     CDS             complement(1361..2263)
                     /locus_tag="PK829_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU41125.1"
                     /translation="MDLSVIVVSYNTCRLLDDCLSSLYAAAPPPGGMEVIVVDNASAD
                     GSVAMVRERYPAVRLIADDVNRGYSAANNRGSAVATGDYLLFLNSDTVVGGDALARPL
                     AYMRANPDVGALTVRLVYPNGERDPDNHRGFPTPWNAFCHFSLLSRLFPNSPRFNGYF
                     RSYEDFSQVHPVPVIAGSYMLMPRALDRQLGGWDETYFFYGEDIDYCYRIQAAGRLIV
                     YYPLVEVVHYKGASSGLRKESADIARPPKATRVKVAEESVRAMRVFYGKFYRDKYPRV
                     VTAFVLAGIQARGWLRIWKHRLSP"
     gene            2451..3545
                     /locus_tag="PK829_07645"
     CDS             2451..3545
                     /locus_tag="PK829_07645"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41126.1"
                     /translation="MIRQLLDDLRAFADYAANHAEESYLTLYLLTDPSHPANQSQTPA
                     WRVFLRNAAAEVEANLDPVQSKQWKNVRLSDTSPEKAWARTRKRLDRYMTTYRTEGKS
                     LALFISPGSAYSFELPVRLDNAYYYGKPHIQEFLWAVDEYEQHFVALFAEDQARLLRV
                     ALSRSAGDTTIVSDQASLRPMRKSADSANIASRADELTRRFVRGVATEADKYFLKTPD
                     AERIILGGNQEIANAVLAQLHPAVQEKVIAVLSIPIDLPAHEVSARIAGAAEQGERDH
                     EAALVAEVIDQARARGRGAKGFTAVGRALERGAVRLIALPYPTPADTAEPILLQAVRA
                     GATVEFLHGEAADAAQAAGGILAQLYYPIN"
     gene            3771..5123
                     /locus_tag="PK829_07650"
     CDS             3771..5123
                     /locus_tag="PK829_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOU41127.1"
                     /translation="MNGKKNNAIYVVGHINPDTDSIAAAMGYAWLLQDQGQNAIAARA
                     GHLNPQTSWVLNHLGIEPPMLLSDASPRFETISHRLNTASPDHPLREVWAIANRTGGV
                     APIVDSEGKPFGLVTVISLFDFLSRTVGSHPRREEMRIGELMDMPGREACDDTVPQFQ
                     AGSRVRDALNRIMREERNEFWVVDDNGRYVGICRQREALNPPRLQVILVDHNEPGQAI
                     GSIDEADIIEIVDHHRLGNPSTRTPIRFTVDVVGSTSTLISERINDAGLSAPPELAGL
                     LLAGLLSDTLILTSPTTTPRDHEAAERLTRWSFVVGSPLAGETVQSYGEKVIASGAGL
                     ATREPEDIVNADLKLYEAGGLTFGVAQAEVTSLVELDEHLERLRDALIKLRDAKGLNF
                     AMLMVTDVVRGSSRLVLTSDVPGLGGLPYAKLPDGTLRAQGVVSRKKQLLPALLSALE
                     "
     gene            5136..>5513
                     /locus_tag="PK829_07655"
     CDS             5136..>5513
                     /locus_tag="PK829_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU41128.1"
                     /translation="MGFIFWQCRDDACRLRFPAEAATAEAGLVCPRCRGPAAVVAVVP
                     RAPLSPPDGAPRARLIALLDNIRSIHNVGSMFRTADGAGLDHLHLAGISATPEHPRLA
                     KAALGAHLTVPWAYHRNSLDAATR"
BASE COUNT          967 a         1855 c         1789 g          902 t
ORIGIN      
        1 atcagcgcgg ccaggtgcgg cggcagcgcg ccaatgcgcg tcggctggat gccgttgcga
       61 tccaccaggc agggcacttc gacacaggct ccctgcggca ggttgtcgat gaggtggtcg
      121 ttggccacgt tgccgtagac gacacgcggc tggcccgtct ccaggctgtg gatgatgtac
      181 gcgccgtact cgtgcgtccg ctccaccggc aaggtgatgg actcgtcttc caacagttgc
      241 cggatggcgt cccaggcggc gatctggttc tcgcagcggg tgatgtactc gtccagaggg
      301 atgttgtagc ggtcgatcag gtcgggccgg tcgcgcttga tataccaggg cacgtattcg
      361 ctgaggtgtt cgctcgactc ggtgacgaag tagccgaagc ggccgaagag gtcatagcgc
      421 acgcggttcc agtcgggcac gcgcccctcg gccagcaccc ggcggatgcg cgggtagagg
      481 tcttccgtgc ccgcagccgt acgccgctcg aaacgcaggt aaaaggccat gtggttaatg
      541 ccggcacaaa ggtagttgat ctcctcaaca ggcacgccga tgtcgcgggc caactgttcg
      601 gccgtgccct gcacactgtg gcacaggccg atggtgcgga tggatgtcgc ccggctcagc
      661 gcccaacagt tgatcgccat cgggttgacg tagttgagca gcgtggcgtt ggggcacagc
      721 gcttccatct cgccggccat gtcgagcaag acgggcacgg tgcgcagccc gcgcatgatg
      781 ccgccgatgc ccagtgtatc ggcgatggtc tgccgcaggc cataacgcct ggggatgtcg
      841 aagtcgatga ccgtcgcggg gcggtagccg ccgatctgga acatggtgat cacgtagtcg
      901 gccccgtcga gagcgcggcg gcggtcggcc gtggcctcaa tgaccggcgc ggcggccagc
      961 gacgcggcca cacgccgggc gacccgctcc gacgtgcgca gacgctcggc gtcgatgtcg
     1021 tgcaggctga tggtggcgcg gctcagttcg ggaaagctca ggatgtcgcc gagcaggttt
     1081 ttggcgaaga cggtgctgcc ggcgccgatg aaagcgatct tgggcatagg ggcatctccg
     1141 ttaagatttg gcgctgatta tagcccgcaa ccacgcgaac gcgatctgct aacgaggcgc
     1201 aaacttagaa gaagcgctca cgcaaagccg caaagaacgc agagttaaga aggcttctta
     1261 agcttggcgc acttggcgtc tttgcgtgag cattcttctt cgcgcccttt gcttactttg
     1321 cggctttgcg tgagcgtctt cttgcacggc cctcggacaa ctaggggctg agccggtgct
     1381 tccagatgcg cagccagcca cgggcctgga tgccggccag gacgaaggcg gtgacgacgc
     1441 gcgggtactt gtcgcggtag aacttgccat aaaagacgcg catggcgcgc accgactcct
     1501 cggccacctt gacccgcgtg gccttgggcg gccgggcgat gtcggccgac tccttgcgca
     1561 ggccggaact ggcccccttg tagtgcacca cctccaccag cgggtaatag acgatcaacc
     1621 ggccggcggc ctggatgcgg tagcagtagt cgatatcctc gccgtagaaa aagtaggtct
     1681 cgtcccagcc gccgagctgc cggtcgagcg cgcgcggcat cagcatgtac gacccggcga
     1741 tgacgggcac gggatgcacc tggctgaagt cctcgtagct gcggaagtag ccgttgaagc
     1801 gcgggctgtt gggaaagagg cggctgagca gagaaaagtg acagaaggcg ttccacggcg
     1861 tgggaaagcc gcggtggttg tcggggtcgc gctcgccgtt ggggtagacc agtcgcacag
     1921 tcagcgcgcc aacatcgggg ttggcgcgca tataggccag cggccgggcc agcgcgtcac
     1981 cgccgaccac cgtgtcggag ttcaggaaga gcaagtagtc gcctgtggcc acggccgagc
     2041 cgcggttgtt ggccgccgaa tagccacggt tgacgtcatc ggcgatgaga cggacggccg
     2101 gatagcgctc gcgcaccatg gccacgctgc catcggccga ggcgttgtcg acgacgatga
     2161 cttccatgcc gcccggcggc ggcgcggcag cgtagagtga gcttaggcag tcgtcgagca
     2221 ggcggcaggt gttgtaggag acgacgatga cggagagatc catactccgg attgtatctc
     2281 aaacggggcg cgctcgcggt cacaagcagc tttgtagggc tttggcggag catcgcaccc
     2341 ggaccaaaag tatgatatgc tctggcctga cctgtattac ctgccttata tgcaaatcca
     2401 agccccggcg cggccgcgtt gaggctaaca tcgaggagaa catcgccacc atgatccgac
     2461 aattgctcga cgacctgcgt gctttcgccg attacgccgc caatcacgcc gaagagtcct
     2521 acctgaccct ctacctgctg accgacccca gccacccggc caaccagagc cagacgcccg
     2581 cctggcgtgt cttcctgcgc aacgccgcgg ccgaggtcga agccaacctc gaccccgttc
     2641 agagcaagca atggaagaac gtccgcctga gcgacacgtc gccagagaag gcctgggcgc
     2701 ggacacgcaa gcggctggac aggtacatga ccacctaccg caccgaaggc aagtcgttgg
     2761 ccctgttcat cagccccggc agcgcctaca gctttgaact gcccgtgcgg ctggacaacg
     2821 cctactacta cggcaagccc cacatccagg agtttctctg ggccgtggac gagtacgagc
     2881 agcacttcgt cgccctcttc gccgaagacc aggcccgtct gctgcgcgtc gccctcagcc
     2941 gctcggccgg tgatacgacg atcgtctccg accaggcgtc gctgcgcccg atgcgcaaat
     3001 cggccgactc ggccaacatc gcctcgcgcg cggacgaact gacccgccgc ttcgtccgcg
     3061 gcgtggccac cgaggccgac aagtacttcc tgaagacacc cgatgccgag cggatcatcc
     3121 tggggggcaa ccaggagatc gctaatgccg tcctggcgca actgcacccg gcggtgcagg
     3181 aaaaagtcat tgccgtcctg tctatcccca tcgacttgcc ggcccacgaa gtctcggccc
     3241 gcattgccgg cgcggccgaa cagggcgagc gcgaccacga ggcggcgctg gtggccgagg
     3301 tcattgacca ggcgcgggcg cgcgggcgcg gagccaaggg cttcaccgct gtgggccgcg
     3361 ctctggaacg cggcgcggta cggctcatcg ccctgcccta ccccaccccg gccgacacag
     3421 ccgagccgat tctgttacag gctgtgcgcg ccggcgcgac cgtggaattc ctccacggcg
     3481 aggcggccga cgcggcccag gccgccggcg gcatcctggc gcaactgtac tatcccatca
     3541 actgacccac cacgtagccc acgggccgcg gagggccggc acatcgatgt gccggccctc
     3601 cgcggttatt gcgcttcacc cacaaccgac gatgcccgcc gccattcctg tctgcccgtt
     3661 cgcccgcaat gtgctagact ccctgttagt aaagtcgtaa ggcacgccca ctagactctc
     3721 tacggttgcg cgaccggcgc ggccgcgtca tcaatccgga acttgaaggt atgaacggca
     3781 agaagaataa cgccatctac gtcgtcggac acattaatcc cgacacggac tccatcgccg
     3841 cggccatggg ctacgcctgg ctgctgcaag accagggcca gaatgccatc gccgcccgcg
     3901 ccggccacct gaacccacaa acgagctggg tgcttaacca cctgggcatc gagccgccga
     3961 tgctgctcag cgacgcctcg ccccggttcg agaccatctc ccaccgcctc aacaccgctt
     4021 cgcccgatca ccccctgcgc gaggtctggg ccatcgccaa ccgcaccggc ggcgtggccc
     4081 ccattgtgga cagcgagggc aagccctttg gtctggtgac ggtcatcagc ctgttcgact
     4141 tccttagccg cacggttggc tcgcacccgc ggcgcgagga gatgcgcatc ggcgagctga
     4201 tggacatgcc cggccgcgag gcctgcgacg ataccgtgcc ccaattccag gccggcagcc
     4261 gcgtccgcga cgcgctcaac cgcatcatgc gcgaggagcg caacgagttc tgggtggtgg
     4321 acgataacgg tcgctacgtt ggcatctgcc gccagcgcga ggcgctcaac ccgcctcggt
     4381 tgcaagtcat cctcgtcgac cacaacgagc cgggccaggc catcggctcc atcgacgagg
     4441 ccgacatcat cgagatcgtc gaccaccacc gcctgggcaa cccctcgacg cgcacgccca
     4501 tccgcttcac cgtcgacgtc gtcggcagca cgtcaacact catctccgag cgcatcaacg
     4561 atgctggtct gagcgcgccg ccggagctgg ccgggctact gctggccggg ctgctgtcag
     4621 acacgctcat cctgacctcg ccgacgacga caccacgcga ccacgaggcc gccgagcggc
     4681 tgacacgctg gtcgtttgtt gtcggttcgc ccctggccgg tgagacggtg cagagctatg
     4741 gcgagaaggt catcgcctcc ggcgcgggcc tggccacacg cgaaccagag gacatcgtca
     4801 acgctgactt gaagctctat gaagccggcg gcctgacctt tggcgttgcc caggccgaag
     4861 tgacctcgct cgtggaactg gacgagcatc tggagcggct gcgcgacgcg ctcattaagc
     4921 tgcgcgacgc caagggactg aacttcgcca tgctgatggt caccgacgtg gtgcgcgggt
     4981 cgagccggct ggtgctgacg tccgacgttc cggggttggg cggtctgccc tatgccaagc
     5041 tgcccgacgg cacgctgcgc gcccagggcg tcgtctcgcg caagaagcaa ctcttgccgg
     5101 cgctgctgag cgccctggaa taggaccgcg cggcgatggg tttcatcttc tggcagtgcc
     5161 gcgacgacgc ctgccggttg cgctttccgg ccgaagcagc aacggctgag gcgggcctgg
     5221 tctgcccgcg ctgccgcggg ccggcggcgg tcgttgccgt cgtgccccgt gcgcccctgt
     5281 ccccgcccga cggcgcgccg cgtgcccgtc tcatcgctct gctggacaat attcgcagta
     5341 tccacaacgt tggctcaatg ttccgcacgg ccgacggcgc tggtctcgac cacctgcacc
     5401 tggccggcat ctcggccacg ccggagcacc cgcggctggc caaggccgct ctcggcgcgc
     5461 acctaaccgt cccgtgggcc tatcaccgca atagtctgga cgcggcgacc agg
//
LOCUS       DAOSMQ010000192         5509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14898_length_5509_cov_4.451045, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000192 DAOSMQ010000000
VERSION     DAOSMQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14898_length_5509_cov_4.451045"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PK829_07660"
     CDS             complement(<1..864)
                     /locus_tag="PK829_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOU41129.1"
                     /translation="MAAGCPLPEAGPETGLHVLKGLARERSLLTALEIMHAEVGPAFR
                     ITLPGFQPAVLVGADSNKQIMVSQREHLNFRSETDPVTKLLRHGVLVEDGHSHACLRG
                     HMEPVLLKPQVVHHVPRMVTYTDQVTEGWAGGSVIDMLIEMRRLALLIFMGTLVDVEF
                     GPDLERLWRPILRAIKYISPGLWIVLPNMPRFGYRAALDQLDAYLYALVRQRRAQLAG
                     CAPDEAARPAHDLLTQLVRVPEMTDDLIRDQILTMLIAGHDTSTALLVWTLHLLSAHP
                     DVMGRAVAEVDA"
     gene            complement(866..2404)
                     /gene="crtI"
                     /locus_tag="PK829_07665"
     CDS             complement(866..2404)
                     /gene="crtI"
                     /locus_tag="PK829_07665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOU41130.1"
                     /translation="MSNKVIIIGSGFGALGAAARLAARGYEVEIFEKRDKPGGRAYVY
                     EQDGFKFDGGPTVVTAPFMFDDIFAAAGRRREEYVEFRALDPFYRIFDHTGRSFDYND
                     DAEFIADQIRHWNPADADGYHRFMHSTQAIFQKGFVELADKPFLTVGDMLRVAPDLIR
                     LQSHRSVYSYVSQFIHNDFLRRCFTFHPLLIGGNPFDTSSIYAMIHYLEREWGIHYVM
                     GGTGALVNALVQLIEELGGRFHYNSEVAEIVVNGRRAEGIRLADGTVHRADHVISNAD
                     VAWTYLNLIPGRARGLRNSDLRYRKLTRYSMSLVVIYFGTKKLYRHDGKLAHHNIILS
                     ERYQGLLADIFKAKTLPRDFSLYLHMPTLTDPSIAPAGHESFYVLSPVPHLGADVDWA
                     TAARPYRDAIMQFLEDNYLPDLQANIVTEHMIDPRHFEQTLNSHLGAAFSIEPVLTQS
                     AWFRPHNRSEDIDNLYFVGAGTHPGAGLPGVLSSSVIAEDLIVTADHKAARVARPRLA
                     ANAG"
     gene            complement(2397..>2600)
                     /locus_tag="PK829_07670"
     CDS             complement(2397..>2600)
                     /locus_tag="PK829_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41131.1"
                     /translation="RFAIQAAAELYAAILDDIEAHDYDVFSRRARTNDSRKLRLLPGI
                     WRRARAARPDLLESMEREHSNNE"
     assembly_gap    2601..2700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2701..3029)
                     /locus_tag="PK829_07675"
     CDS             complement(<2701..3029)
                     /locus_tag="PK829_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOU41132.1"
                     /translation="MAVQLDLWEHSLLAQAYAPLENRLRHGAELVAGSAQSRRAYDHC
                     RAITRQHSRTFFIASSLMRAEHRRAIQALYAFCRVSDDIVDRNDGDRPADLRAWRQRS
                     LSSHPGS"
     gene            complement(3258..3686)
                     /locus_tag="PK829_07680"
     CDS             complement(3258..3686)
                     /locus_tag="PK829_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOU41133.1"
                     /translation="MKRLTRAECWDFMMARPHTGKLATIRPDGRPHVVPIWFERDGET
                     IVFTTGTTTVKATNMRHNSAVCLCVDDETPPFTYVKVDGTVTFSVDLDALRHWATRIG
                     GRYMGQEQAEAYGARNGVPGELLVRLTPTALFGEDNIAGW"
     gene            complement(3709..4266)
                     /locus_tag="PK829_07685"
     CDS             complement(3709..4266)
                     /locus_tag="PK829_07685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU41134.1"
                     /translation="MSQHRNPYRGVADRRADSILIRPALPEDAAQVIAYARSVFAEPG
                     VNLITAPDEFNPTTEAEARMIVEMNRSSNSLFLVAEDDGRIVGQLTLQGGKRRHVRHA
                     ATLGITVAQEWRNYGVGRRLMAYAIDWARASGVLTRIELFVFVRNTGAIHLYESCGFE
                     VEGRRRRSTFRDGEYLDDLLMSLLL"
     gene            complement(4269..>5509)
                     /locus_tag="PK829_07690"
     CDS             complement(4269..>5509)
                     /locus_tag="PK829_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOU41135.1"
                     /translation="RAERHAFDPYAQTASRLRALETIGHPTGKVELLVLGGTWSSYRR
                     DYQEWFIKRCFDALNGFEAADLPAAQAANAVGRRRNVGLVVETRQDHIDAGELRWFRY
                     LGVTKVQIGIQSLDERVLALNRRGHDVQATRDAFRLLRLAGFKIHGHWMANLLGATPA
                     GDLADYARLWDDPAIRPDELKLYPCMLLANAELYDYWRRGEYAPYSEEELIDLLARCK
                     AQTPRYARLTRIVRDFPTTNVVAGNKKANLRQLAQERLREAGTPCRCIRCREIRRDPL
                     PAALGLHDLTYDTDATTEHFLSFDSDAGFPAADRLAAFLRLSLPRRDQPQPFPELAGA
                     AMIREVHVYGPALNPGDDSRGEAQHSGLGMALIERAAAVARAESYTRLAVISAVGTRE
                     YYRRLGFAPDGLYMSRPLEG"
BASE COUNT          892 a         1648 c         1936 g          933 t
ORIGIN      
        1 ggcatcgact tcggccacgg cgcggcccat cacgtcgggg tgggcactga gcaaatgcag
       61 cgtccagacc agcagtgccg tgctggtgtc gtgcccggcg atgagcatgg tcagaatctg
      121 gtcgcgaatg agatcgtcgg tcatttcggg cacgcgcact agctgggtca gcagatcgtg
      181 ggccgggcgc gcggcctcgt ccggcgcgca gccggccagt tgcgcccgcc gctgccggac
      241 gagggcgtag aggtaggcat cgagctgatc cagcgcggcc cggtagccga agcggggcat
      301 gttgggcagc acaatccaca gcccgggcga gatgtacttg atggcccgca ggatcggccg
      361 ccacagccgc tccagatcgg ggccgaactc gacatcgacc agcgtgccca tgaagatgag
      421 cagagccagc cggcgcatct cgatgagcat atcgatcacc gagccgccgg cccaaccctc
      481 ggttacctgg tcggtgtagg tcaccatccg cggcacgtgg tggacaacct gcggcttcag
      541 cagcactggc tccatgtgac cgcgcaggca ggcatggctg tggccgtctt ccaccagcac
      601 gccgtgacgc agcagcttcg tcaccgggtc ggtctcgcta cgaaagttga gatgctcgcg
      661 ctgcgacacc atgatctgct tgttgctgtc tgccccaacg aggacagccg gttggaagcc
      721 gggcagggtg atgcgaaagg ccgggcccac ctctgcgtgc atgatctcca gcgcggtcag
      781 cagcgagcgc tcgcgggcca ggcccttcag gacgtgaagg ccggtttccg ggccggcttc
      841 gggcaggggg cagccagcag ccatcttaac cagcgttggc cgccagccgc ggtcgggcca
      901 cgcgcgcggc tttgtgatcg gcggtgacga tcagatcttc ggcgatgacc gacgaggaca
      961 gcaccccggg cagccccgcg ccggggtgcg tgcccgcgcc gacgaagtag aggttgtcga
     1021 tgtcctcgga gcggttgtgc ggccggaacc aggccgattg cgtcagcacc ggctcgatgg
     1081 agaaggccgc gcccagatgg ctgttgagcg tctgctcgaa gtggcgtggg tcgatcatat
     1141 gctccgtgac aatgttggct tggaggtcgg gcaggtagtt gtcctccagg aactgcatga
     1201 tcgcgtcgcg gtagggcctg gccgccgtgg cccagtccac atccgcgccc aggtgcggca
     1261 cgggcgagag gacgtagaag ctctcgtggc cggccggggc gatggacggg tcggtcagtg
     1321 tgggcatgtg caggtagagc gagaagtcgc gcggcagcgt cttggccttg aagatgtcgg
     1381 ccagcagccc ctgatagcgc tcgctcagga tgatgttgtg gtgggccagc ttgccgtcgt
     1441 ggcgatagag cttcttcgtg ccgaagtaga tgaccaccag cgacatgctg tagcgggtca
     1501 gcttgcggta gcgcaggtcg ctgttgcgca ggccgcgcgc ccggccgggg atgaggttca
     1561 ggtaggtcca agccacgtcg gcattggaga tgacgtggtc ggcgcggtgg acggtgccgt
     1621 cggccaggcg gatgccctcg gcccggcggc cgttgacgac gatctcggcc acttcgctgt
     1681 tgtagtggaa gcggccgccc aactcctcga tcaattgcac cagcgcattg accagcgcgc
     1741 ccgtgccgcc catgacgtag tggatgcccc actcgcgctc caggtagtgg atcatggcgt
     1801 agatcgacga cgtgtcgaac gggttgccgc cgatgagcag cgggtggaac gtgaagcagc
     1861 gtcgcaggaa gtcgttgtgg atgaactgcg acacgtagct atagaccgac ctgtgcgact
     1921 gcaagcggat caggtcgggg gccacgcgca gcatgtcgcc cacggtcagg aacggcttgt
     1981 cggccagttc gacgaagccc ttctggaaga tggcctgagt cgagtgcatg aagcggtgat
     2041 agccatcggc gtcggccggg ttccagtggc ggatctggtc ggcgatgaac tctgcgtcgt
     2101 cgttgtagtc gaatgagcgg ccggtgtggt cgaagatgcg gtagaacgga tcgagggcgc
     2161 gaaactcgac gtactcctcg cggcggcggc cggcggcggc gaagatgtcg tcgaacatga
     2221 acggcgcggt gacgaccgtc gggccgccgt cgaacttgaa gccgtcctgc tcgtagacgt
     2281 aggcgcgccc gccgggcttg tcgcgtttct cgaagatctc gacctcgtag ccgcgcgcgg
     2341 ccagccgggc ggccgcgccc agcgcgccga agccgctgcc gatgatgatg actttgttac
     2401 tcattgttgc tgtgctccct ctccatagat tccagcaggt ctggccgggc agcgcgggca
     2461 cgccgccaga tgccgggtag tagacgcaac ttgcggctgt cgttggtgcg ggcgcgacga
     2521 ctgaagacgt cgtaatcgtg ggcttcgatg tcgtcgagga tggcggcgta tagctcggcc
     2581 gcggcctgga tggcgaagcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 tcgctgccgg ggtggctgct caggctgcgc tgccgccagg cgcgcaggtc ggccggcctg
     2761 tcgccgtcgt tgcggtcaac gatgtcgtca ctgacgcggc agaaggcgta gagagcctgg
     2821 atggcgcggc ggtgctcggc gcgcatcagg ctggaggcga taaagaaggt gcggctgtgc
     2881 tggcgggtga tggcacggca atggtcgtaa gcccgccgcg actgggcgct gccggcgacc
     2941 aattcagcgc cgtggcgtag acgattctcc agcggggcat aggcctgcgc cagtagactg
     3001 tgttcccaaa gatcgagttg gactgccatc cgctttctcc ttcgcctggt tcggcccata
     3061 cacaatagcc aagccagcca ctttgcgcca tcaccgccaa tggacggcgc tacagtggca
     3121 agatagcaga ttgcccataa tttgtcaata tattgtacaa aggttggtgg ggtggctagt
     3181 ggcggctcgg cgcaagagga gcaatagtct acccaaaggc taactacctg gccaaaaatg
     3241 gcgggccacg gggcggacta ccagccggcg atgttatcct cgccaaagag agccgttggc
     3301 gtcaggcgaa cgagaagctc gcccggcacg ccattgcgcg cgccataggc ttcggcctgc
     3361 tcctggccca tgtagcgccc accaatgcgc gtggcccagt ggcgcagggc gtccaggtcg
     3421 acgctgaacg tcaccgtgcc gtccactttt acgtaggtga agggcggcgt ttcgtcatcg
     3481 acgcacagac agaccgccga attgtggcgc atgttggttg ccttgaccgt cgtggttcca
     3541 gtggtgaaaa caatcgtttc gccgtcgcgc tcgaaccaga tgggcacgac gtggggacgg
     3601 ccgtcggggc ggatggtggc cagcttgcca gtgtgtggcc gggccatcat aaagtcccag
     3661 cattcggcgc gagtcagtcg cttcatgatg tttctctccc gtcgcccgct agagcagcag
     3721 cgacatcaac agatcatcca gatattcgcc gtcgcggaag gtcgagcggc ggcgacggcc
     3781 ctctacctcg aagccacacg attcgtatag atggatcgcg cccgtgttgc gcacgaagac
     3841 aaacaattcg atgcgcgtca ggacaccgct ggcgcgcgcc caatcaatgg catacgccat
     3901 cagccgccgg ccgacgccgt agttgcgcca ttcctgggcc acggtgatgc ccagcgtggc
     3961 cgcgtggcgg acatggcggc gcttgccgcc ttgcaacgtc aactggccga cgatgcggcc
     4021 gtcgtcctca gccaccagga agaggctatt gctcgaccgg ttcatctcga cgatcatgcg
     4081 cgcctcggcc tcggtcgtcg ggttgaactc gtccggggcg gtgatgagat tgacgcccgg
     4141 ctcggcgaag acgctgcggg cgtaggcgat cacctgggcg gcatcttcgg gcagggcagg
     4201 ccggatgagg atgctgtcgg cgcggcggtc ggccacgccg cgataggggt tccggtgttg
     4261 actcatggtc atccctccag cggccgggac atatacaggc catcgggcgc gaagccgagc
     4321 cgccggtaat actcgcgcgt gcccacggcg ctgatgacgg ccaggcgggt gtagctctcg
     4381 gcgcgagcga cggccgcagc ccgctcgatg agcgccatcc ccaggccgct atgctgggct
     4441 tcgccgcggc tgtcgtcgcc ggggttgagc gccgggccgt agacgtggac ttcgcggatc
     4501 atggccgcgc cggccagctc cgggaagggc tgcggctggt cgcggcgtgg cagggagagg
     4561 cgcaggaagg cggccagccg gtcggcggct gggaagccgg catcgctgtc gaagctgaga
     4621 aagtgctcgg tcgtggcgtc cgtgtcgtag gtcaggtcgt ggaggccgag ggcagccggc
     4681 agaggatcgc gacggatttc gcgacagcgg atgcagcggc agggcgtccc cgcttcgcgt
     4741 agtctttcct gagccaactg gcgcagattg gccttcttgt tgccggcgac aacgttggtc
     4801 gtcgggaagt cgcgcacgat gcgcgtcagg cgggcgtagc gcggcgtctg cgccttgcaa
     4861 cgggccagca ggtcgattag ctcttcttcg ctatagggcg catactcgcc gcgccgccag
     4921 taatcgtaca gctcggcgtt ggccagcagc atgcacgggt agagctttag ctcgtcgggg
     4981 cggatggccg gatcatccca taggcgggcg tagtcggcca ggtcgccggc cggcgtcgcc
     5041 cccagcagat tggccatcca gtggccgtga atcttgaagc cggccagtcg cagcagccgg
     5101 aaggcgtcgc gcgtggcctg gacatcatgg ccgcgccggt tgagggccag cacgcgctcg
     5161 tcgaggctct gaatgccgat ctgcaccttg gtgacgccca ggtagcggaa ccagcgcagc
     5221 tcgccggcat cgatgtggtc ctggcgcgtt tcgacgacaa gcccgacgtt gcgccgccgg
     5281 ccgacggcgt tggcggcctg ggccgcgggc aggtcggccg cctcgaagcc gttgagcgcg
     5341 tcgaagcagc gtttgatgaa ccactcttga tagtcgcgcc ggtagctcga ccacgtgccg
     5401 cccagtacca gcagttcgac cttgccggtg gggtggccga ttgtctccag cgcgcgcagg
     5461 cgggaggcgg tctgggcgta ggggtcaaaa gcgtggcgct cggcccgtt
//
LOCUS       DAOSMQ010000193         5506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_14908_length_5506_cov_3.939277, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000193 DAOSMQ010000000
VERSION     DAOSMQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14908_length_5506_cov_3.939277"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PK829_07695"
     CDS             complement(<1..473)
                     /locus_tag="PK829_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU41136.1"
                     /translation="MAHYPYLIIGGGMTGAAAANAIRERDAAGLIGLISAEPEPPYDR
                     PPLSKKLWDGKRTIDQIYVKLPGNVQAMLGRRVVAIDPEARRVTLDDGASHTYGRLLL
                     ATGGDPRRLPFGGAHVNYYRTLADYRALRADADAGARFIVIGGGFIGSEVAASLR"
     gene            662..>1071
                     /locus_tag="PK829_07700"
     CDS             662..>1071
                     /locus_tag="PK829_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41137.1"
                     /translation="MSNSVRTPRSTRLLYAAGSLGVALSYQAFSTYIQFLYIDVLGVR
                     AVWIGVVWAIYGVWNAVNDPLSGYWSDRTRTRWGRRVPWIAGSLVPLLITFFLLWFPF
                     GKAVAWSELTTLFYFLGIVLLFDLFWTVYVMNWTA"
     assembly_gap    1072..1171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1172..1921
                     /locus_tag="PK829_07705"
     CDS             <1172..1921
                     /locus_tag="PK829_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41138.1"
                     /translation="RDFVAFLGANLMIEYVFLGLAAAIPFYAKYVLAIQGPTTLLGLT
                     LDAETQNSVLLAAAFLAALPAMAIWWALARRFGAWRALRAACLMGAATALYFFFPRTF
                     QAGLLGTTAFGLSLAGLLMLTNPLIADITDEDELHTGARREGMFFGMNGLVIRFAFVI
                     QGILTAIVFTVTGYINPTAGVLFPEQPASAIFGMQLLTGGFTTLGLVLAFVFLSFYGL
                     HGARAERVRAEAAALQARKRAALDAELGARG"
     gene            complement(1939..2718)
                     /gene="surE"
                     /locus_tag="PK829_07710"
     CDS             complement(1939..2718)
                     /gene="surE"
                     /locus_tag="PK829_07710"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU41139.1"
                     /translation="MHILVTNDDGVHAPGLAALVDAMKPLGRVTILAPDHNWSGGGHV
                     KTLHRPLRVLETTLADGTPALASDGAPSDCVALALLGRVAEKIDLVVSGINTYGNLGH
                     DVTYSGTVTAAMEAVIAGVPGFAFSLAADDMTRERDYATAAAYAQRIVAAAIAQGLTR
                     DLLLNVNVPAVPAAGIHGIRVTRLGLRVYRDRLDARRDPRGVPYYWIGGDYPTGVPDP
                     GTDFEALRDGYVSVTPLQLDLTCHSTVERLAAWSWQIGARG"
     gene            complement(2822..3202)
                     /locus_tag="PK829_07715"
     CDS             complement(2822..3202)
                     /locus_tag="PK829_07715"
                     /inference="COORDINATES: protein motif:HMM:NF025445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HOU41140.1"
                     /translation="MSERTERPRLSLPRALLHLEGAALLLLATGAYFRLGQPWWLFLL
                     LLLAPDLSLLGYLAGPRVGAVAYNVVHTMVGPLLLLAAGWWLGAGTAVAVALVWLAHI
                     GMDRLVGYGLKYPDEAKSSHFSRI"
     gene            complement(3215..3490)
                     /locus_tag="PK829_07720"
     CDS             complement(3215..3490)
                     /locus_tag="PK829_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41141.1"
                     /translation="MCCIVSLLVFIGPRIAAIAWYLTDTARWSAAFNNILLPLLGVVF
                     LPWTTLAYVLMSPGGVQGLQWAVVVLGFLFDIGALGSGGYSNRRRHN"
     gene            complement(3573..4442)
                     /locus_tag="PK829_07725"
     CDS             complement(3573..4442)
                     /locus_tag="PK829_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOU41142.1"
                     /translation="MPKKDKARKKPAAPSVRELLRVGPDFDPAAIDARRVMAGPADKV
                     QALAQVGALAGEVSELQERLYAESKGGGKRRLLVLLQGMDTSGKGGAIKALDRIVEPL
                     GMRVAQFGAPTKAELKKHYLWRIEQKLPPPGTIGIFDRSHYEDVLVVRVRNLVPPEEW
                     EKRYDEINAWEKKWSDDGVVFLKCMLFISPAEQKARLLARLAEPDKRWKYRPGDVDDR
                     LLWDDFMTAYQAALTRCSTEYAPWYVIPADRKWHRDWLLTHMVAETLREMNPQYPSPD
                     YDVAAETKRVMAS"
     gene            complement(4461..4706)
                     /locus_tag="PK829_07730"
     CDS             complement(4461..4706)
                     /locus_tag="PK829_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41143.1"
                     /translation="MRPRRRASWPAKNCVAFGLFTLKRWAWLLALLGVAITFITGIMG
                     LFSGGLIAFCCGVLGLIVSGAILYYLLQPDVRRVFGR"
     gene            complement(4672..5112)
                     /locus_tag="PK829_07735"
     CDS             complement(4672..5112)
                     /locus_tag="PK829_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOU41144.1"
                     /translation="MRRNTRVVVQPRRGPGLMGTMARTAVIAGTATAVSGATANKMQQ
                     SAQAQQMQQQAQVQAMVDQKLAEQQALTEQQQLQQQLAEQQAQLQQLMQQQMAPQAAA
                     APIEAGPSAAEVRIMKLKELGALRDAGILTEAEFEAEKARILAG"
     gene            complement(5141..>5506)
                     /locus_tag="PK829_07740"
     CDS             complement(5141..>5506)
                     /locus_tag="PK829_07740"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HOU41145.1"
                     /translation="VVPALAELVEQQFVHILDLVFVHKSEDGTMTAGEVEALDPELGA
                     VFARLEGDIDDLLNEEDLSDIAAEMAANSVAVVIVWENLWAARFAEAVRASGGELVDS
                     GRIPHDVAVAAMTYSGIED"
BASE COUNT          865 a         1866 c         1724 g          951 t
ORIGIN      
        1 ttgcgcagcg aggcagccac ctctgagccg atgaagccac cgccgatgac gatgaaacgc
       61 gcccccgcgt cggcgtcagc gcgcagggca cggtagtcgg ccagcgtgcg gtagtagttg
      121 acgtgcgcgc cgccgaacgg cagccggcgc ggatcgccgc cggtggccag cagcaggcgg
      181 ccgtaggtgt ggctcgcgcc gtcgtcgagc gtcacccggc gcgcctccgg gtcgatggcg
      241 acgacgcgcc ggccgagcat ggcctgcacg ttgccgggca gtttgacata aatctgatcg
      301 atggtgcgct tgccgtccca gagctttttc gacagcggcg gccggtcgta gggcggctcc
      361 ggctcggccg agatgaggcc gatgaggccg gcggcgtcgc gctcgcggat ggcgttggcc
      421 gccgccgcgc cggtcatgcc gccgccgatg atcaggtagg gataatgggc cattgatttg
      481 ctctcctttc tgccgggctt accgcggcca ttttagcacg aaatcgccct ggcagggttc
      541 gccgcgcgtg gcgcgattct ccgaatcgca gagcttgcga ttcggagaat cgcgccacac
      601 ccattgccgc gccgccgttc tgggctagaa ttcgtcctca gtccacacga acgagtcgcc
      661 catgtctaac tctgtccgca cccctcgctc cacgcgcctc ctctacgccg ccggctccct
      721 cggcgtggcc ctgtcctacc aggcgttctc cacctacatc cagttcctct acatcgacgt
      781 cctcggcgtc cgcgcggtct ggatcggcgt cgtctgggcg atctatggcg tttggaacgc
      841 ggtcaatgac ccgctctccg gctattggtc tgaccgcacg cgcacgcgct ggggccgccg
      901 cgtaccctgg atcgccggct cgctcgtgcc gctgctcatc actttcttcc tgctgtggtt
      961 cccttttggc aaggccgtgg cctggtcgga gttgaccacg ctgttctact tcctgggcat
     1021 cgtcctgctg ttcgaccttt tctggacggt ctacgtcatg aactggacgg cnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcgacttc gtggccttcc tgggcgcgaa
     1201 cctgatgatc gagtacgtct tcctgggcct ggccgcggcc atccccttct acgccaagta
     1261 cgtcctggcc atccaggggc cgacgacact gctcggcctg acgctggacg cggagacgca
     1321 gaactcggtg ctgctggcgg cggccttcct ggcggcgttg ccggcaatgg ctatctggtg
     1381 ggcgctggcg cggcgcttcg gggcctggcg ggcgctgcgg gcggcctgcc tgatgggcgc
     1441 ggccacggcg ctctacttct tcttcccgcg caccttccag gccgggctgc ttggcaccac
     1501 cgctttcggt ctcagcctgg ccggcctgct gatgctgacc aacccgctca tcgccgacat
     1561 caccgacgaa gacgaactcc acaccggcgc gcgccgcgag ggcatgttct tcggcatgaa
     1621 tggcctggtc atccgcttcg ccttcgtcat tcagggcatc ctgacggcca tcgtcttcac
     1681 cgtcaccggc tacatcaacc cgaccgccgg cgttctcttc ccggagcaac cggcctcagc
     1741 catctttggg atgcagttgc tgaccggcgg cttcaccacg ctgggcctgg tgctggcctt
     1801 cgtctttctg agcttctatg ggctgcacgg cgcgcgcgcg gagcgcgtcc gcgccgaggc
     1861 cgcggcgctg caagcgcgca agcgggccgc gctcgatgcc gaactagggg ctagaggcta
     1921 ggggaagagt gctcttccct agcccctagc ccctatctgc cacgaccacg ccgccagccg
     1981 ctcaaccgtg ctgtggcagg tcaggtcaag ctgtaacggc gtcacgctga cgtagccgtc
     2041 gcgcagagcc tcgaagtcgg tgccggggtc gggcacgccg gtggggtagt cgccgccaat
     2101 ccagtagtaa ggcacgccgc gcgggtcgcg gcgggcgtcc agccggtcgc ggtagacgcg
     2161 cagccccagc cgggtcacgc ggatgccgtg gatgccggcc gcgggcacgg cgggcacatt
     2221 gacattcagc agcaggtcgc gcgtcaatcc ctgggcaatg gccgcggcca cgatccgctg
     2281 ggcataggcc gcggcggtgg catagtcgcg ttcgcgcgtc atgtcgtcgg cggccagcga
     2341 gaaggcgaag ccgggcacgc cggcaatgac cgcctccatc gccgccgtca ccgtgccgct
     2401 ataggtcacg tcgtggccca ggttgccata ggtgttgatg cccgacacga ccaggtcgat
     2461 cttctccgcc acgcggccga gcagcgccag ggccacgcag tcggacggcg cgccgtcgct
     2521 ggccagggcg ggcgtgccat cggccagcgt cgtctccagc acacgcagcg gccggtggag
     2581 ggtcttgacg tgtccgccgc cagaccagtt gtggtcgggg gccagaatgg tgacgcggcc
     2641 aaggggtttc atggcgtcca ccagggcagc cagccccggc gcatggacgc cgtcgtcgtt
     2701 ggtaacaagg atatgcatga ccgggattat gcccctatcg ccggcaattg ggcaggggaa
     2761 ttggacgcca gtggactcag gtgagaaggc gcgtctaggc agtcagtggg ctgtggcccg
     2821 ctcagatgcg actgaagtgg gacgacttgg cctcgtcggg gtacttcagc ccatagccaa
     2881 ccaggcgatc catgccgatg tgggccagcc agacaagagc caccgccacg gccgtgcccg
     2941 cccccaacca ccaaccggcg gccagcaaca gcagcggccc gaccatcgta tgcacgacgt
     3001 tataggccac agccccaacg cgcgggccgg ccaaatagcc gagcaggctc aggtcagggg
     3061 ccagcagcag caacaggaac agccaccacg gctggcccag ccggaagtac gcccccgtcg
     3121 ccagcagcaa cagcgccgcg ccctccaggt gcaagagcgc ccgcggcagg cttagccgcg
     3181 ggcgctcggt gcgttctgac agatctttca cggactagtt gtgacggcgg cggttgctat
     3241 agccgccgct gcccaacgcg ccaatgtcga acagaaaacc gaggacgaca accgcccact
     3301 gtaagccctg caccccgccg ggcgacatga gcacataggc cagcgtcgtc cacggcaaaa
     3361 agacgacgcc cagcaacggc agcaagatgt tattgaacgc cgcgctccag cgagccgtgt
     3421 cggtcagata ccaggcgatg gcagcgatgc gcgggccgat gaagacgagc agactgacta
     3481 tacaacacat ggtcttacct cctgtcctat aatacgcgct gctgccgggg cggttgcgcc
     3541 aaaggggcat tgcgccgcgg cagcgttgcg ccctagctgg ccataacgcg cttggtctcc
     3601 gcggccacgt cgtagtctgg cgacggatac tgcgggttca tctcgcgcaa cgtctcggcg
     3661 accatgtggg tcagcagcca gtcgcggtgc cacttgcggt cggccggaat gacgtaccac
     3721 ggcgcatact ccgtcgagca gcgcgtcagg gccgcctgat aggcggtcat gaaatcgtcc
     3781 cacagcaggc ggtcgtccac atcgccgggg cgatacttcc agcgcttgtc cggctcggcc
     3841 aaccgggcca gcaggcgcgc cttctgctcg gccggggaga tgaagagcat gcacttcagg
     3901 aagacaacgc catcgtcgct ccacttcttt tcccaggcat tgatttcgtc gtagcgcttc
     3961 tcccactcct ccggcggcac caggttgcgc acgcgcacga cgaggacatc ctcatagtgc
     4021 gagcggtcga agatgccgat tgtgcccggc gggggcagct tctgctcaat gcgccacagg
     4081 taatgcttct tcagttctgc cttcgtcggc gcgccgaact gggcgacgcg catgcccagc
     4141 ggctcgacga tgcgatccag cgccttgatg gcgccgccct tgcccgacgt gtccatgcct
     4201 tgcagcaaga caagcagccg gcgcttgccg ccacctttcg attcggcata gagtcgctct
     4261 tgcaactcgc tcacttcgcc ggccagcgcg ccgacctggg ccaacgcctg caccttgtcg
     4321 gccgggccgg ccatgacgcg ccgcgcgtca atcgccgccg ggtcgaagtc tggcccgacg
     4381 cgcaacaact cgcgcacgga tggcgcggcg ggtttcttcc tggccttatc tttcttgggc
     4441 attacttcct tcccgacggc ctagcgtccg aacactcgtc tgacgtctgg ctgcaataga
     4501 tagtaaagga tcgccccgga cacgatcaac cccaacacgc cacagcagaa ggcgatcaac
     4561 ccgccgctga agaggcccat gatgccggtg atgaacgtga tggccacgcc caacagcgcc
     4621 agcagccacg cccaacgctt cagggtaaac agaccaaagg cgacacagtt cttagccggc
     4681 caggatgcgc gccttctcgg cctcaaactc ggcctcggtc aggatgccgg cgtcgcgcag
     4741 cgcgcccagc tcctttagct tcatgatgcg cacctcggcc gcgctggggc cggcctcaat
     4801 gggcgcggcg gccgcctggg gggccatctg ttgctgcatg agctgctgca actgggcctg
     4861 ctgttcggcc agttgttgct gcaactgctg ttgttccgtc agggcctgct gttcggccag
     4921 tttttgatcg accatcgcct gcacctgggc ctgctgttgc atctgctggg cctgggccga
     4981 ctgctgcatc ttgttggcgg tcgcgccgga gacagccgtg gctgtgccgg cgatgacggc
     5041 cgtgcgggcc atggtgccca tcagtcccgg gccgcggcgc ggctgaacga cgactcgtgt
     5101 gttccttctc atggatgtgc ctcgttggta ttactatcag ctagtcttca atgccagagt
     5161 acgtcattgc tgcgacggca acgtcgtggg gaatgcgacc gctatcgacc agttccccgc
     5221 ccgacgcgcg caccgcctca gcaaagcgcg ccgcccacag gttctcccag acgatgacca
     5281 cggccacgct gttggcggcc atctccgcgg caatgtcact caggtcttcc tcgtttaaca
     5341 gatcgtcaat gtcgccttcc agccgggcga acacggcccc caactcgggg tcaagcgcct
     5401 ccacttcgcc cgccgtcatc gtcccgtcct cgctcttgtg aacgaagacc aggtcaagga
     5461 tgtggacaaa ctgctgctcg accagctcgg ccagcgccgg caccac
//
LOCUS       DAOSMQ010000194         5473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15024_length_5473_cov_3.893688, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000194 DAOSMQ010000000
VERSION     DAOSMQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15024_length_5473_cov_3.893688"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PK829_07745"
     CDS             <1..599
                     /locus_tag="PK829_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU41146.1"
                     /translation="PLPDDPALEQQYVNKIAAGIDRMSALVEGLLEMANLRAGTRLQF
                     DRVAVGEVLAEIAAEYASPAWMRGVRLIVEAPADLPPVKADPTLLRRALTNYVTNALK
                     YAPESGPVRLRAEARGDEVVLSVIDRGAGIDPADLPHLFQRFYRGSQGAATERPDGSG
                     LGLAIVQSIADLHGGRVWCESEPGQTTAFFLSLPAWRG"
     gene            complement(608..1003)
                     /locus_tag="PK829_07750"
     CDS             complement(608..1003)
                     /locus_tag="PK829_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41147.1"
                     /translation="MEEFLRNFGLVLLGVAGVAGVLALILLVIIIVQVRRVHVPANAG
                     LAETLLYTPFLVVVFLDVLDLSLDFLSAPIAWIVLDRLGLKGLRGFATVESLVPFTQV
                     IPTMTLLWIGVRLFGKDRFQEDRGYDSRW"
     gene            complement(1108..1608)
                     /locus_tag="PK829_07755"
     CDS             complement(1108..1608)
                     /locus_tag="PK829_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOU41148.1"
                     /translation="MLDALGESFVLLVVALAFGVGLLGAFLPILPGTLIVWAGVLFYA
                     WPFDGFQTFSPWVFALITLVAIVSGTADLWLPLLGAKKTGASWRTLVVGLIGGILGTF
                     LLPLPVFGTIVGYGAGVLVAEYVRHGAWRPAWRATLGGVVGWGLSAAVELAGAMIMIL
                     LFFTQA"
     gene            complement(1676..2956)
                     /locus_tag="PK829_07760"
     CDS             complement(1676..2956)
                     /locus_tag="PK829_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOU41149.1"
                     /translation="MEPLPVDVLQLQRRDPVAWTALLSRAPEMADVIVTAVMAEPLWD
                     TALVLPGRARAERARHMRRYVLTLDGSSDPITFIAKQTNAVEALLYHALGEPPATGIV
                     PCHYAHVDGDASWVILEDVPDDFPPSVWQPAHVDAIIATLARVHAAHWRKDITGIGAI
                     PHFLHRPEGPYSWEALQGGRPTLFEDGPGAVLSRHAVRRAGRLAPLFLEAANGLVALR
                     DLGGWPGVLDESHLAAAADLLDDPVPLLATLDDLPWTLIHGAPHADHWRLTLFEEQYL
                     IDWSTAQHGPGVLDLIAFLERFPLLYERLPEGGDEEEDGRPRVRLREIAPLFEETAID
                     TYLLTLSAEMGQHFSARRFRAAVPAARCLHVLTTWFPFFARWSDNMPGKATWQRLNQL
                     SEADLAQSRVGPMAGLRPYLAGVFDRFLHAYRSL"
     gene            3260..>5473
                     /locus_tag="PK829_07765"
     CDS             3260..>5473
                     /locus_tag="PK829_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU41150.1"
                     /translation="MDSRLSRWCAGLLEAGWLIAILAVPLFFNIQSERVFEPDKLALL
                     RSLVLVMAAIWLVGFIDRRGWLDRGWLRPSNPDALWHRPLVLPVLAVVVIYVLASLFS
                     VSPRVSWLGSYQRLQGTYTTLAYIAVFALMAATIRTPEQVRRAVTVAIVTSIPVALYG
                     LLQHFGQDPLPWGGDVEARVAGHMGNAIFIAAYLIMIVPLTLGRAMDALNGILSEERL
                     AAADVARASAYLFTLAIQLLTIYWSGSRGPLIGLGVGLFAFTLVLLVSMRGAATEGRR
                     GRDALAAVAFALPAVLALLLSPLVARAAGPAVAFGFFFGVVALSIMAVFVLAALRRGR
                     RWLWLAWMLLTIFMASWLLLFNVPAERTAGLRGVPVAGSVLNVFDEWRALPTVGSFGR
                     MLDPTDTTGREKSGRVRILIWQGVVKLISFHTPLQFPDGHVDPFNWLRPILGYGPETM
                     YVAYNRFYPPELATVEARNASPDRSHNETFDALVITGLLGLVAWQALFLSVILFAFRA
                     LGVVGSRRDGWMLVGLWVGGAVAAALLAMFLVGPIYLGVAVPVGVILGVVAYLIYQAL
                     FGRTEPPEASDSAHSFASDRLLMNALVAAVLAHYVEIHFGIAISATRLHFFVYAALML
                     ALVSVRARPAAAPVQATAAAAPSGKRRRKAAATSADAAARPALWSKLLVPALLLTLMV
                     GILGYNFITYALPPGKTITGPADLSAVEVFRQSLTQNARADFAGSPFVLSLLVLSW"
BASE COUNT          903 a         1819 c         1840 g          911 t
ORIGIN      
        1 tgccgctgcc cgacgacccg gcgctggagc agcagtacgt caacaagatc gccgccggca
       61 tcgaccgcat gtcggcgttg gtggagggct tgctggaaat ggccaacttg cgcgcaggca
      121 cgcggctgca attcgaccgc gtggccgtgg gcgaagtgct ggccgagatc gccgcggagt
      181 acgcttcgcc ggcgtggatg cgcggcgtcc gcctcatcgt cgaggccccg gccgacctgc
      241 ccccggtcaa ggctgacccg acactactgc ggcgggcgct gaccaactac gtcaccaatg
      301 cgctgaaata cgcgccggag agcggcccgg tgcggctgcg ggccgaggcg cggggggatg
      361 aggtcgttct cagcgtgatc gaccgcgggg cgggcatcga cccggccgac ctgccgcacc
      421 tcttccagag gttctaccgc ggcagccagg gcgcggcgac cgagcgaccg gacggctctg
      481 gcctggggct ggccatcgtc cagtcaatcg ccgacttgca tggcggccgg gtgtggtgcg
      541 agagcgaacc gggccagacc acggcctttt tcctgtctct acccgcctgg cggggctgac
      601 gggctgctta ccaccgcgaa tcgtagccgc ggtcttcctg aaagcgatcc ttgccgaaga
      661 ggcgtacccc aatccacagc agtgtcatcg tggggatgac ctgggtgaaa gggacaagcg
      721 attcaaccgt ggcgaagccg cgcaacccct tcaaacccaa ccgatcgagg acgatccagg
      781 caatgggcgc ggaaaggaag tcaagtgaca ggtcgagcac gtcgaggaag acgacgacca
      841 gaaacggcgt atagagcagc gtttcggcca gacccgcgtt ggcgggcacg tggacacgcc
      901 gcacctggac aatgatgatg accagcaaga tgagggccag gacgccggct acaccggcca
      961 cgcccaagag caccagacca aaattacgca gaaactcttc cataactcaa ggcgcctcac
     1021 atacgccaaa gcggccggcc accatgcggc tcttgcacag agccggccgc cagccgcgct
     1081 atctcgttaa ccaactgctc cgtagggcta tgcctgagtg aagaagagca gaatcatgat
     1141 catcgcgccg gccagttcga ccgcggcgct aaggccccag ccgacgacgc cgcccagcgt
     1201 cgcccgccag gccggccgcc acgcgccgtg gcgcacgtac tcggccacca aaacgcccgc
     1261 gccatagccg acgatcgtcc cgaagacggg caatggcagc aggaacgtac ccagaatgcc
     1321 accaatgaga ccgaccacaa gcgtccgcca cgacgcgccg gtcttcttgg cccccagcag
     1381 cggcagccag aggtcggccg tgccgctgac gatagccacc agagtgatga gggcgaagac
     1441 ccacggggag aaggtctgaa agccatcgaa cggccaggcg tagaacaaca cgcctgccca
     1501 gacgatcagc gtacccggta gaataggcag aaacgcgccg agcaggccaa cgccaaaggc
     1561 cagcgccact accagcagga caaaactctc gcccagtgca tctagcatct tagcatcgta
     1621 ggtctatacg cagacgagcg cccgacgttg cagcggaggg agggaggggc gagaatcaga
     1681 ggctgcggta ggcatggagg aagcggtcga acacaccagc caggtaggga cgcagcccgg
     1741 ccatggggcc gacgcgcgac tgggccaggt ccgcctcgct gagctggttc agccgttgcc
     1801 aagtggcctt gccaggcatg ttatcgctcc aacgggcgaa gaagggaaac caggtcgtca
     1861 acacgtggag gcagcgcgcc gcgggcacgg ccgcccggaa ccgccgcgcc gaaaagtgct
     1921 ggcccatttc ggctgagagc gttagcagat aggtgtcgat ggccgtctcc tcgaacagcg
     1981 gcgcgatctc gcgcaggcga acgcgcggcc ggccgtcttc ctcctcgtcg ccgccctccg
     2041 gcaggcgctc atagagcagc gggaagcgct ccaggaaggc gatgaggtca agcacgcccg
     2101 gcccgtgttg agcggtcgac cagtcaatga ggtattgctc ctcgaagagc gtcagccgcc
     2161 agtggtcagc gtggggcgcg ccgtggatga gcgtccaggg caggtcatca agcgttgcca
     2221 gcagcggcac ggggtcgtca agcagatcgg ccgccgcggc caggtgcgac tcgtcgagca
     2281 caccgggcca gccacccagg tcgcgcaggg ccaccagccc attggctgct tccaggaaga
     2341 ggggcgccag tcggccggcg cggcgcacag cgtggcgcga caggacggcc ccggggccat
     2401 cctcaaaaag cgttgggcgg ccgccttgca gcgcttccca ggaatacggc ccttctggcc
     2461 ggtgcaggaa gtgggggatg gccccgatgc cggtgatgtc tttgcgccag tgggccgcat
     2521 ggacgcgagc gagggtggca atgatggcat ccacgtgcgc cggctgccag accgatggcg
     2581 ggaagtcgtc gggcacgtct tccaagatga cccaactggc gtcgccgtcc acgtgggcat
     2641 aatggcaggg aacaatgccc gtagccggcg gctcgcccag ggcgtgatag agcagcgcct
     2701 cgacggcgtt ggtctgtttg gcgatgaagg tgatcgggtc ggaggagccg tcgagcgtca
     2761 ggacgtagcg gcgcatgtgg cgcgcgcgct ccgcccgggc gcggccgggc aacaccaggg
     2821 ctgtgtccca cagcggttcg gccatgacgg cggtgacaat gacatcggcc atttccggcg
     2881 cgcgcgacaa gagagctgtc caggctaccg ggtcgcggcg ctgtagctgc aagacatcga
     2941 cgggcagggg ttccattgcg gcgaaagtgt agccaagatc gccgattgcg gcaacccacc
     3001 atacacgccg aacaagccag agaacgaatt cctggccgcg gtaatctact ccagaccggc
     3061 tattttccgg cccatccatg cggggctttg ccaaagtcat cgagcgacca ttaacctgac
     3121 aggtgaacgg ccagtcgttc tctggtggca gtcccaggcg tggccgcccc accggtcagg
     3181 tcggtgcgcc tggggtgact ttggaaaagc cctccccatc catgtcctat tcgggcgcac
     3241 tatgctatgc ttaggcccga tggactcacg actctcgcgc tggtgcgccg gtctgttgga
     3301 agcgggttgg ctgattgcca tcctggccgt gcctctcttc ttcaacatcc aatctgaacg
     3361 cgttttcgag ccggacaagc tggcgctgct gcgctcgctg gtgctggtca tggccgccat
     3421 ctggctggtc ggcttcatcg accggcgcgg ctggctggac cgcggctggc tgcgcccgtc
     3481 caaccccgat gcgctctggc accggccgct ggtgctgccg gtgctggctg tggtggtcat
     3541 ctatgtgctg gcgtcgcttt tctccgtgtc cccacgtgtc agttggctgg gttcctacca
     3601 gcgcctgcaa ggcacataca caacgctggc ctacatcgcc gtctttgccc tgatggcagc
     3661 caccatccgc acgccggagc aggtgcgacg cgccgtcacc gtggccattg tcaccagcat
     3721 ccccgtggcc ctctatgggc tgttgcagca cttcgggcaa gacccgctgc cgtggggtgg
     3781 cgacgtggag gcgcgcgtgg ccggccatat gggcaacgcc attttcatcg ccgcctatct
     3841 gatcatgatc gtccccctga cgctgggccg ggccatggac gcgctcaatg gcatcctcag
     3901 cgaggagcgg ctggccgcgg ccgacgtggc ccgcgcctcg gcgtatctct tcacgctagc
     3961 catccaactg ctgacgatct actggtcggg cagccgcggg ccgctgatcg gcctgggcgt
     4021 tggcctgttc gcctttacgc tggtgctgct cgtgtcgatg cgcggcgcgg cgactgaagg
     4081 gcggcgcggc cgagacgccc tggcggcggt ggccttcgcg ctgccagcgg tgctggcgct
     4141 gctcctcagc ccgctggtgg ccagggcggc cgggccggcg gtggccttcg gcttcttctt
     4201 cggtgtcgtg gccctgtcga ttatggccgt cttcgtcctg gcggcgttgc ggcgcgggcg
     4261 gcggtggctg tggttggcct ggatgctgtt gaccatcttc atggccagct ggctgctgct
     4321 gttcaacgtg cccgcggagc gtacggctgg cctgcgcggc gtgcccgtcg ccggcagcgt
     4381 gctgaacgtg ttcgacgagt ggcgcgcgct gcccaccgtt ggctccttcg gccgcatgct
     4441 cgacccgacg gacacgaccg gccgcgagaa gagcggccgg gtgcgcatcc tcatctggca
     4501 gggcgtcgtc aaactgatct ccttccatac gccactccag ttccccgatg gccacgtgga
     4561 tccgttcaac tggctgcggc cgatcctggg ctatggccca gaaacgatgt acgtggccta
     4621 caaccgcttc tatccgccgg agttagccac ggtggaggcg cgcaacgcct cgcccgaccg
     4681 ctcccataac gagacgtttg acgcgctggt gatcaccggc ctgctggggc tggtggcctg
     4741 gcaggcgctt ttcctgagtg tcatcctgtt tgcctttcgc gcgctggggg tcgtcggctc
     4801 gcggcgcgac ggctggatgc tagtgggcct gtgggtgggc ggggcggtgg ccgcggcgct
     4861 gctggccatg tttctggttg gccccatcta cctgggcgtg gccgtgcctg taggtgtgat
     4921 cctgggcgtg gtggcctatc tcatctacca ggcgctcttt ggtcgaacag agccgcccga
     4981 agccagtgac agtgcccact ccttcgcgtc ggatcgattg ctgatgaacg ctctcgtagc
     5041 ggcggtgctg gcccactacg tcgaaatcca ttttggcatc gccatctcgg cgacgcgact
     5101 gcatttcttt gtctacgccg cgctgatgtt ggccctggtc agcgtccgcg cccggccggc
     5161 ggctgccccc gtccaagcga cggccgccgc cgctccgtcg ggcaagcgtc gccgcaaggc
     5221 cgcggctacg tctgcggacg ccgcggcccg gcccgcgctc tggagcaaac tacttgtccc
     5281 cgccctgctg ctgacgctca tggtcggcat cctcggctac aacttcatca cctatgccct
     5341 gccgccgggc aagaccatca ccggcccggc cgacctgtcc gcggtcgagg tcttccgcca
     5401 atcgctgacc cagaacgccc gcgccgactt cgccggctcg ccctttgtgc tgtcgctgct
     5461 ggtgctgtcg tgg
//
LOCUS       DAOSMQ010000195         5455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15077_length_5455_cov_3.757407, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000195 DAOSMQ010000000
VERSION     DAOSMQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15077_length_5455_cov_3.757407"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PK829_07770"
     CDS             <1..921
                     /locus_tag="PK829_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOU41151.1"
                     /translation="YFAISAEDARSVVEQLRAGTDLDSIGVNGEAVTTADDITGIWVS
                     SVESGSPADKVGVKPGDIITMLEGLVLATNGTMSDYCDILRSRNATDPMSIEVLRYDT
                     QEVMEGQLNGYASDPLAQSFSFAQEVAAPEGGESTTSGGTATYENYVGIYDDSQAIYV
                     EVPAEWADVDGSNWVDSDDGSVLGSELIASTDIAGFSGTYETPGMQILAGAYFGDTTM
                     GELVDVFDFSTDCTYDGRFDYSDPVYTGVYDQYSNCAGVGSVIIVLGAEPAAQNYSVV
                     VLVQAVTEADLDALDHILNTFNVVGTLPGQ"
     gene            1044..2609
                     /locus_tag="PK829_07775"
     CDS             1044..2609
                     /locus_tag="PK829_07775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU41152.1"
                     /translation="MTLEIGSVLNSRYRIARLVGQGGFGAVYRAWDLALSQPVALKEN
                     VDSGSESQRQFEQEAKLLAGLRHPNLPRVIDHFILTDQGQYLVMDFIEGRSLAQMLRD
                     RRGPLDEAEAQQWLHQICDAVEYLHTRTPPIIHRDIKPDNIIVTAEGRAMLVDFGISK
                     LYDAQRGTTVGARAVTPGYSPPEQYGFGKTDARSDVYALGATLYTLLTGQPPPEAIDL
                     ISGTEVLTPVQQVNPAVSGAAAQAIVAAMMPSISQRLASAARFEQALTGRVAPPVPAP
                     APQPRPAPPRPDRPPAPAPSAPARPRQRQGCMLGGVAIAALVVGAIAMLVLILWLFAR
                     LGASSTVDFTPTITPFAVATPEAISLSGSYVGVTNNSGALSADAPVEWVEIDGSNWLD
                     SAGNPLGARITVAPNINALRSSYGAPGVDILASVLLSNGSLAELDNYKTLYSTACTYD
                     GRSDISFAAYTGAYDAYSGCGGGQGNVYVAAGTAADGSHIVVFQCQYITQADLEACNH
                     MLNSVAVAGALPQ"
     gene            complement(2614..5439)
                     /locus_tag="PK829_07780"
     CDS             complement(2614..5439)
                     /locus_tag="PK829_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOU41153.1"
                     /translation="MTTTYVAIDLETTGLDPARDAIIEVAAVTFRDNEIVDEFAALVN
                     PLRDIPPYVSQLTGITDAMVADAPTLFSLRSRLKSKLADHVIVGHNVQFDLGFLREAG
                     LGIGNPRLDTITLASILVPEAGRFNLGALAEFLNFPQTDRDGHRALADTEQTIELFLA
                     LRERALALSLAQLEEIVGAGKRLGWPETRFFEDVLAARARHAFEGDELRGRGQLLPLY
                     RAPKLEGRALVPAESPAPLDIPWLMSLIRPGGHFDHAFPGFEHRPQQVAMMRAVAEAF
                     NEGRQVLVEAGTGTGKSLGYLLPAAFWATENGRRVVISTNTINLQDQLVKKDVPALRD
                     ALNLDLRAAVRKGRSNYLCTRLFQQMRRSGPKDADEMTLYARILLWLPHSETGDVSEI
                     ILRTPAERLAWSRLSGENPTCRSEQCATENCPLHIARRRGELAHLVIVNHALLLADMA
                     ANGMVLPEYSDLIIDEAHHLEAAVTDGLSFRADRRFLETILEEASRPRAGLIANAQQR
                     IGGVAPREIVATVDSLAERLRADAQAAIFQTQDWFTTLDFFLRGQERERSQFASQLRL
                     TPGTRAQPDYHLVEEGWADLSRVYAVIVKGFQKVAEILADLSHTVDIEGEDELRAALI
                     SNGQMLEDARQNLDALIARPNAEMIYWAEQYKDNISLHAAPLHVGPLVDKYIFQGKET
                     VVLTSATLRTAGYGRPESTFDYLRHRLNAHDADELAVGSPFDYKNSTLLYLVTDIPEP
                     NQPGYQRMLEAAIVDTAVALGGRTLVLFTANNHLNQTAAAIEDRLAHHNITTLAQGGS
                     ASRQQLLEQFRREDGRSVLLGTRSFWEGVDVPGPALTALLIAKLPFDVPTDPIFAARS
                     ETYENPFFEYSIPEAVLRFRQGFGRLIRRGTDEGVVICLDKRLLTKRYGELFLEALPA
                     CTVVRQRADRIAELVQRWLARDRNRKL"
BASE COUNT          926 a         1796 c         1812 g          921 t
ORIGIN      
        1 tacttcgcca tcagcgccga ggacgcgcgg tcggtcgtcg agcaactgcg cgccggcacc
       61 gatctcgact ccatcggcgt aaacggcgag gccgtcacca ccgccgacga catcactggt
      121 atctgggtct cctcggtcga atccggctcc ccggccgaca aggtcggcgt caagccgggc
      181 gacatcatca ccatgctgga aggcctggtg ctggccacga atgggacgat gtcggactac
      241 tgcgacattc tgcgcagccg caacgccacc gaccccatgt cgatcgaagt cctgcgctac
      301 gacacgcaag aggtgatgga agggcaactg aacggctacg ccagcgaccc gctggcgcag
      361 tccttctcct ttgcccagga agtcgccgcg cccgaaggcg gcgaatcgac caccagcggc
      421 gggacggcca cctatgagaa ctacgtcggc atctacgacg actcacaggc gatctacgtc
      481 gaagtgccgg ctgagtgggc ggacgtggac ggctctaact gggtcgatag cgacgacggc
      541 tcggtgctgg gcagcgaact gatcgcctcg accgacatcg ccggcttcag cggcacctac
      601 gagacgcccg gcatgcagat tctggccggg gcttacttcg gcgacacgac gatgggcgag
      661 ctggtcgatg tctttgactt ctcgaccgac tgcacctacg acggccgctt cgactactcc
      721 gacccagtct ataccggcgt ctatgaccag tactccaact gcgccggcgt gggcagcgtc
      781 atcattgtcc tgggcgctga gccggcggcc cagaactact ccgtcgttgt cctggtgcaa
      841 gccgtcactg aggctgacct ggacgcgttg gaccacatcc tgaacacgtt caacgtcgtc
      901 ggcaccctgc ccggtcaata atgtagacag gcaatgagtg ggggcgggca gtgaatgccc
      961 gccccactca tcttcgtccg gttgggcggc gcgcgtcgag ggggcgctcg ctgccgccgt
     1021 caggtgtcgc actcagaggg aacgtgacac tagaaatcgg ctctgtcctc aacagtcgct
     1081 accgtatcgc ccgcctcgta ggccagggtg gttttggcgc ggtctatcgc gcctgggacc
     1141 tggccctgtc gcagccggtg gctctgaagg aaaacgtcga cagcggctcc gaatcgcaac
     1201 gccagtttga gcaggaggcc aaattactgg ccggtctgcg ccaccccaac ctgccccgcg
     1261 tcatcgacca cttcatcctg accgatcagg gacaatacct ggtcatggac ttcatcgagg
     1321 gccgcagcct ggcccagatg ttgcgcgacc ggcgcgggcc gctggatgag gccgaggcac
     1381 agcaatggct tcatcagatc tgcgacgccg tggagtattt acacacgcgc acgccgccga
     1441 tcatccaccg cgacattaag cccgataaca tcatcgtcac cgccgaaggc cgggccatgc
     1501 tggtagactt cggcatctcc aagctctacg atgcccagcg cggcacgact gtgggcgcgc
     1561 gcgccgttac gcccggctac agcccgccag agcagtacgg ctttggcaaa accgacgcgc
     1621 gcagcgatgt ctacgcgctg ggggcgacgc tctacaccct gctgaccggc cagccgccgc
     1681 cggaggccat cgatctgatt agcgggaccg aggtattgac gcccgtgcag caggtcaacc
     1741 cggccgtcag cggcgcggcc gcccaggcca tcgttgcggc gatgatgccg tccatcagcc
     1801 agcgattggc cagcgccgcc cgctttgagc aggcgctgac cggacgcgtt gccccgccgg
     1861 tgcccgcgcc cgcgccccag ccccggccag cgccgccccg gccagatcgg ccgccagcgc
     1921 cggccccttc ggccccggcc aggccgcgcc agcgacaggg ctgtatgctg gggggcgtcg
     1981 ccattgccgc cctggtcgtc ggcgcgatag cgatgctggt gttgatcctg tggctgttcg
     2041 cgcggctcgg cgcgtcgtct accgtggact ttacgcccac gataacgccc tttgccgtgg
     2101 ccacaccgga agcgatcagc ctcagcggca gctacgttgg cgtgacgaac aattccggcg
     2161 cgctttcggc cgacgcgcca gtggaatggg ttgaaatcga cggctcgaac tggctcgact
     2221 ccgccggcaa cccgctgggg gcgcgtatca cggttgcgcc gaacattaat gccttgcgta
     2281 gttcttatgg cgcgccggga gtggatatcc tggccagcgt cttactgagc aacggcagcc
     2341 tggctgagct tgataactac aaaacactct atagcacggc ctgcacctac gacggccgct
     2401 ccgacatcag cttcgcggcc tacaccggcg cttacgacgc ctattccggc tgcggcggcg
     2461 ggcaaggcaa cgtctatgtc gccgccggca cagcggccga tggctcccat atcgtcgtct
     2521 tccagtgcca gtacatcact caggccgacc tggaggcatg caaccatatg ctgaacagtg
     2581 tcgccgttgc cggcgcgttg ccccagtagg aaactacaac ttgcggttgc ggtcgcgggc
     2641 cagccagcgc tgcaccagct ccgcgatacg gtcggcccgc tggcgaacga ccgtgcaggc
     2701 cggcaacgcc tccaggaaca gctcgccata gcgcttggtc agcaggcgct tgtccagaca
     2761 aatcaccacg ccttcgtccg tgccgcgccg gatgagccgg ccgaagccct gccggaagcg
     2821 cagcactgcc tcggggatgg agtactcgaa gaaggggttc tcgtaggtct ctgagcgcgc
     2881 ggcgaagatg gggtcggtgg gcacgtcgaa gggcaacttg gcgatgagca gcgccgtcag
     2941 ggctggcccg ggcacgtcta ccccctccca gaaagagcgc gtacccaaca ggacgctccg
     3001 cccatcctcg cgccggaact gctctagcag ttgttgccgc gaggcgctgc cgccctgggc
     3061 cagggtggtg atgttgtggt gggccaggcg gtcttcaatg gccgcggccg tctggttgag
     3121 gtggttgttg gctgtgaaca gcaccagcgt ccgcccgccc agggcgacgg cagtgtccac
     3181 gatggccgct tccagcatcc gttggtagcc gggctggttc ggttccggga tgtcggtcac
     3241 caggtagagc agcgtggagt tcttataatc gaagggcgaa ccaacggcca actcgtcggc
     3301 gtcgtgggcg ttgagccggt ggcgcagata gtcgaaggtc gattccggcc gcccatagcc
     3361 ggccgtgcgc aacgtggccg aggtcagcac gaccgtctcc ttgccctgga agatgtactt
     3421 atctaccaat gggccgacgt gcagcggcgc ggcgtggaga gagatgttgt ccttgtactg
     3481 ttcagcccag tagatcatct cggcgttggg ccgggcgatg agcgcgtcga ggttctgccg
     3541 ggcgtcttcg agcatctgcc cattactaat gagcgccgcg cgcagttcgt cctcaccctc
     3601 gatatcaacc gtgtggctta gatcggccag gatttcagcc accttctgga agcccttgac
     3661 gatgacggcg tagacgcgac tcaggtcggc ccagccctct tcgacgagat gataatcggg
     3721 ctgcgcgcgc gtgccgggcg tcaggcgcaa ctgcgaggcg aactggctgc gctcccgctc
     3781 ctggccccgc aggaagaagt cgagcgtggt gaaccaatcc tgcgtctgga agatggccgc
     3841 ctgggcgtcg gcgcgcaacc gttcggccag gctatccacc gtggcgacga tctcccgcgg
     3901 ggcgacgcca ccgatgcgct gctgggcgtt ggcgatcagg ccggcgcgcg gccgcgaggc
     3961 ttcctccagg atggtctcca ggaagcggcg gtcggcgcgg aagctgaggc cgtcggtgac
     4021 ggccgcctcc aggtggtggg cctcgtcgat gatcaggtcg ctatactctg gcaagaccat
     4081 gccattggca gccatgtcgg ccagcagcag cgcgtgattg acgatgacca gatgggccaa
     4141 ttcgccgcga cggcgggcga tgtggagggg gcaattctcc gtggcgcact gctccgacct
     4201 acacgtgggg ttctcgccgc tgagccgcga ccacgccagc cgctcggccg gcgtccgcag
     4261 gatgatctcg ctgacgtcgc cggtctcgct gtggggcagc cacagcagga tgcgggcata
     4321 gagcgtcatc tcgtcggcgt cctttggccc gctgcggcgc atctgttgga aaaggcgggt
     4381 gcacaggtag ttgctgcgcc ccttgcgcac ggccgcgcgc aggtcgaggt tcagggcgtc
     4441 gcgcagcgcg ggcacgtcct tcttgaccag ttggtcttgt agattgatcg tgttggtgga
     4501 aatgaccacg cgccggccgt tctccgtggc ccagaaggcg gccggcaaga ggtagccgag
     4561 gctcttgccc gtgcccgtgc ccgcctccac caggacctga cggccctcgt tgaaggcctc
     4621 ggccacggcg cgcatcatcg cgacctgctg cgggcggtgc tcgaagccgg gaaaggcgtg
     4681 gtcgaaatgg ccgcccggcc ggataagcga catcagccag gggatgtcca gtggcgcggg
     4741 cgactcggcc ggcacaagcg cccgcccctc cagcttgggg gcgcggtaga ggggcagcaa
     4801 ttggccgcgg ccgcgcagtt cgtccccctc gaaagcgtgg cgcgcccgcg cggccagcac
     4861 gtcctcgaag aaccgcgttt ccggccaacc cagccgtttc cccgcgccaa cgatctcctc
     4921 cagctgggcc aatgacagcg ccagcgcccg ctcgcgcagg gccaggaaca gctcaatcgt
     4981 ctgctccgtg tcggccaacg cacggtggcc gtcgcggtcg gtctggggga agttcaggaa
     5041 ctcggccagc gcgccgaggt tgaagcgtcc cgcctctggc acgagaatgg acgccagcgt
     5101 gatcgtatcg aggcgcggat tgccgatgcc caggccggcc tcgcgcagga agccgagatc
     5161 gaactgcaca ttgtggccga cgatgacgtg atcggccagc ttgctcttca gccgcgagcg
     5221 caggctgaaa agagtgggcg cgtcggcgac catcgcgtcg gtaatgccgg tcaactggct
     5281 gacgtagggc ggaatatcac gcaggggatt gaccagcgcg gcgaactcat cgacgatttc
     5341 attgtcgcgg aaggtgacag cggcgacttc gatgatggcg tcgcgggcgg ggtccaggcc
     5401 ggtcgtctcc aggtcaatgg ccacgtaggt ggtcgtcatg gagagggatt atacc
//
LOCUS       DAOSMQ010000196         5403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15258_length_5403_cov_4.676889, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000196 DAOSMQ010000000
VERSION     DAOSMQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15258_length_5403_cov_4.676889"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PK829_07785"
     CDS             <1..1542
                     /locus_tag="PK829_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41154.1"
                     /translation="DADDNALEGMAIVATDGSHGSWQFSLANPPAGEANWQPAGVVSS
                     SSALLLPDTAWLRFVPAANYVGPSGEMTFRAWDQTSGAAGQRVDASQTGGSSAFSTAT
                     GTVVATVTPVNDPPALTGLSASALPYAEDASPTRLMPGVVVVDVDNAVLAGATVRLTN
                     PLDGDAEQLSAAPGSTGIAAVYEDGALRLSGAASLAAYQQVLRSVAYHNTSQDPDATP
                     RILAVTAADALLSSPAYTLTVQVQPVNDPPALDLDSGSAGNDSEAVFYINRAPVRVAP
                     NLVLTDVDNTNLKSVTVRIVNLKNPQAELLTADVSGAANIKREYDPATGVLKLERMDS
                     VVNYQQVLSTLTYVNTLPQPDLEDRRIEFTVSDSDAAGGTRVAIIHLLPAPAARLLMP
                     MISRRGEEPNDVCADAYAILPNRTESFLPDDSVDWFTFDLPAAADVAIELRGFSPGRG
                     QMNVAVGAACQQLQLIGTAGEPTPDKTVNLGRREAGRYYVRVIADGPLSETTAYTLFV
                     RATAP"
     gene            1620..2588
                     /locus_tag="PK829_07790"
     CDS             1620..2588
                     /locus_tag="PK829_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HOU41155.1"
                     /translation="MDNTAQRELFPELPRAEDERKPAPLTRAASLQAALGTFEKHMQD
                     EGFSLNTVKAFASDIRLLGRYLGIGTPIGEIGTKNLTDFLHWLTDERGVPCSPKSYAR
                     RVTALKVFFGWLHDTGVLAQDPSTAVVQQSVSSPLPSAPTAVDLERALVVVEARRAGA
                     DGHKPDARPTLLLNLLLQTGIKKGEAMAIVPNHIDASDASAPMLYIRYANPRLRYKER
                     KLPLEPEWLRVLDEYMQQYRPSDTLFTCTARNLEYVLRDIAEAGDLPVGALSFENLRW
                     ASAMRDWRAGVEQDDIRQKLGLSKIAWRETKSKLQKLIDHEATTMA"
     gene            complement(2585..2974)
                     /locus_tag="PK829_07795"
     CDS             complement(2585..2974)
                     /locus_tag="PK829_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41156.1"
                     /translation="MDIRFFDDPLEQPRGREDTRFRQIGLFVYPERRVFAFGVELTPF
                     LERPSIEVHVRNGVGEPAGSLHVIETLTPNFSLIMHLRDTATLNPYELTAVLYYAWPD
                     QGRIEVDHQTVQFNMDEAGEHRFPFED"
     gene            complement(2980..>5403)
                     /gene="mfd"
                     /locus_tag="PK829_07800"
     CDS             complement(2980..>5403)
                     /gene="mfd"
                     /locus_tag="PK829_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU41157.1"
                     /translation="SRQAPRLAELWAGEGGKLIRTLEPLTQAGPSDAVDPVVTAIDAG
                     TGRAGTVSLSSLPEAPPDGSLTFVHGSLADGFSLVRREDHRVLLHLLTDAEIFGWSRP
                     APRRQRTARPAAPEAYFADIKQGDYVVHLDYGIGKFEGLVVRNLGGMEREYLLVQYAN
                     ADTLYVPVHHADRLGKWIGPDDADPSLNRLGERTWTQARAAAQRAADELAQELLDLYA
                     ARETVPGHAFPPDSEWQAELEAAFPYRETEDQLRVIGEVKDDMERANPMDRLICGDVG
                     YGKTEVALRAAFKAVNDGKQVAMLVPTTILAQQHFTTFRERLTPFPVTVEMLSRFRTA
                     TQQRRIVQQVRAGQVDIIIGTHRLLSDDIAFKDLGLVIIDEEQRFGVADKEKLKQWRT
                     EVDVLTLTATPIPRTLHMALTGVRDISIIDTAPAERLPVETYVGESDDARIRTAILRE
                     LDRGGQVFFVHNRVQSIHIIHKRLEKLAPEARIVVAHGQMSERQLEKIMVEFAEARVD
                     ILLSTTIIESGLDFPNANTLIVDRAEQFGLSQLYQLRGRVGRSTRRAYAYFFHAPWRS
                     LTPDAQARLETIAEHTQLGSGYFIAVRDMEIRGAGDFLGGEQSGHISAVGFDMYTRLL
                     TRAVKRRKAEQRGEAPPTELPDNVLIDLPLATYIPTDYVPDTQLRLRLYRRMATLDTL
                     AEIDEMAAELADRFGPIPDPVHNLLYQLRAKSLGQMAGVNAVVTEVGQIRIRIAELGG
                     MDRYRLQRYLGENVRVSKSAIWLKKDRGTHEWQIELVQVLEKLRLFERTPGATPERGA
                     AGTPADSGQ"
BASE COUNT          913 a         1765 c         1802 g          923 t
ORIGIN      
        1 gacgccgacg acaacgcgct ggaaggcatg gccatcgtgg ccaccgacgg cagccacggt
       61 agctggcagt tcagcctggc caacccaccg gcgggcgagg ccaactggca gccggccggc
      121 gtcgtctcgt cgtcgtcggc gctcctgttg cccgacacgg cctggctgcg ctttgtgccc
      181 gcggccaact acgtcggtcc ctccggcgag atgactttcc gcgcctggga ccagaccagc
      241 ggcgcggccg gccagcgcgt cgacgcctcc cagacgggcg gcagcagcgc ttttagcacg
      301 gctacgggca ctgtcgtggc caccgtcacg ccagtcaacg acccgccggc gctgacgggg
      361 ctgtcggcga gcgccctgcc ctatgccgaa gacgccagcc cgacgcgcct gatgcccggg
      421 gtggtcgttg tcgatgtgga caacgctgtc ttggcggggg ctaccgtgcg gttgacgaat
      481 ccgctcgatg gcgacgccga gcagctatcg gccgcgcccg gcagtacggg aatcgcggcc
      541 gtctatgagg acggcgcgct acggctcagc ggtgccgcct cgctggccgc ctatcagcag
      601 gtgctgcgct cggtggccta ccacaacacc tcgcaagacc ccgacgccac gccgcgcatt
      661 ctcgccgtca ccgcggccga cgcgctgctc agctcgccgg cctacacgct aaccgtgcag
      721 gtgcagcccg tcaacgatcc ccccgcgctc gaccttgaca gtggcagcgc gggcaacgac
      781 agcgaggcgg tcttctacat caaccgcgcg ccggtgcgcg tcgcgccgaa cctggtgctg
      841 accgatgtgg acaacaccaa cttgaagagc gtcacggtcc gcatcgtcaa tctgaagaac
      901 ccacaagcgg agctactgac ggccgacgtt agcggcgcgg ccaacattaa gcgcgagtac
      961 gacccggcca ccggcgtgct gaagctggaa cgcatggaca gcgttgtcaa ctaccagcag
     1021 gtcttaagca cgctgaccta tgtcaatacc ttgccgcagc ccgatctcga agaccggcgc
     1081 atcgaattta ctgtcagcga cagcgatgcc gccggtggga cacgggtggc gatcatccac
     1141 ctgctgcccg cgccggcggc gcgcctgctc atgccgatga tctcgcggcg cggcgaggaa
     1201 ccaaacgacg tctgcgccga cgcctatgct attctaccca atcgcaccga gtcgtttctg
     1261 ccagatgaca gcgtggactg gtttaccttt gacctgccgg cggcggccga tgtcgcgatt
     1321 gagttgcgcg gctttagccc cggtcgcggc cagatgaacg ttgctgtggg cgcggcctgc
     1381 cagcagctgc aacttatcgg cacagccggc gagccaacgc cggataaaac ggtcaatttg
     1441 ggccggcgcg aggcaggccg ctactacgtt cgcgtcattg ccgacgggcc gctgagcgag
     1501 acaaccgcct acaccctatt tgtccgcgcg actgcccctt gacaaaagag gtctcctcgg
     1561 tctacaattt cgcataatgg tcttcgcata actgatatgg cgacggccga gggaaacgca
     1621 tggacaatac agcacagcgg gaactattcc cagaactgcc gcgcgccgaa gacgagcgca
     1681 agcccgctcc cctgacacgc gcggcgtcct tgcaggcagc gctgggcaca tttgagaagc
     1741 acatgcagga cgagggcttt tcgctcaaca cggtgaaggc cttcgccagc gatattcgct
     1801 tgctgggccg ctacctgggc atcggtacgc ccatcggcga gatcggcaca aagaacctga
     1861 ccgacttcct ccactggttg accgacgagc gtggcgtgcc ctgtagcccc aagtcctacg
     1921 ctcgccgcgt gacggcgctg aaggtcttct tcggctggct gcacgacacc ggcgtcctgg
     1981 cgcaggatcc gtcaacggcg gtcgtccagc agagtgtcag cagtcctttg cccagcgcgc
     2041 cgacggccgt tgatcttgag cgcgccctgg tcgtggttga ggcgcgacgc gccggcgcag
     2101 acggccacaa gcccgacgct cgccccactc tgctgctcaa tttgctgttg cagacgggca
     2161 ttaaaaaggg cgaagcgatg gccattgtgc ccaaccacat cgacgccagc gacgccagcg
     2221 cgccgatgct ctacattcgc tacgccaacc cgcgcctgcg ttacaaggaa cgcaagctgc
     2281 cgctggagcc ggagtggctg agggtgctcg atgagtatat gcagcagtac cggcctagcg
     2341 acaccctctt cacctgtacg gctcgcaacc tggagtacgt cctgcgtgac atagccgagg
     2401 ccggcgatct acccgtgggc gcgctgtcgt ttgagaatct gcgttgggca tcggccatgc
     2461 gcgactggcg ggccggcgtc gagcaagacg acattcgcca gaagctgggt ctgtctaaga
     2521 tcgcctggcg cgagacaaag agcaagctcc agaagctcat cgaccacgaa gcgacaacga
     2581 tggcctagtc ctcgaacggg aagcggtgtt cgccagcctc gtccatgttg aactggacgg
     2641 tctggtggtc aacctcaatg cggccctggt cgggccaggc atagtagagg acggccgtca
     2701 actcataggg gttcagcgtc gctgtgtcgc gcaggtgcat gatcaggctg aaattaggtg
     2761 tcagcgtctc gatgacgtgg agcgagccgg ccggctcgcc cacgccgttg cggacgtgga
     2821 cttcaatcga cggccgctca aggaacggtg tcaactccac gccgaaggcg aacacccgcc
     2881 gctccggata aacaaacagg ccgatttggc ggaagcgcgt gtcctcgcgc ccgcgcggct
     2941 gctccagggg gtcgtcaaag aatcgaatgt ccattgtctt cactgcccac tgtctgccgg
     3001 cgtgccggct gcgccccgtt cgggtgttgc cccgggcgtg cgttcaaaca ggcgtagttt
     3061 ctccagcact tgcaccaact cgatctgcca ttcgtgcgtg ccgcggtctt tcttcagcca
     3121 gatggcgctc ttgctaacgc gcacgttttc gcccaagtag cgttgcaagc gatagcggtc
     3181 catgccgccc agctcggcaa tgcggatgcg aatctgtcca acctcggtga cgacggcgtt
     3241 gaccccggcc atctgcccca acgacttggc ccgcagctgg tagagcaggt tgtgcaccgg
     3301 gtcggggatg gggccgaaac ggtcggccaa ctccgcggcc atctcgtcga tctccgccag
     3361 cgtgtccagc gtggccatac gccggtagag gcgcagccgc agttgcgtgt cgggcacgta
     3421 gtcggtgggg atgtaggttg ccagaggcag gtcgatgagc acattgtcgg gtaactcggt
     3481 cggtggggcc tcgccgcgct gctcggcctt gcggcgcttg acggcgcgcg tcagcagccg
     3541 ggtgtacatg tcgaagccga cagcggagat gtggccgctc tgctcgccac ccaggaagtc
     3601 gcccgcgccg cgaatctcca tgtcgcgcac ggcgatgaaa tagccggagc cgagttgagt
     3661 gtgctcggcg atggtttcca aacgggcctg ggcatcgggc gtcagcgagc gccagggggc
     3721 atggaagaag taggcatagg cgcggcgcgt ggagcggccg acgcgcccgc gcaactggta
     3781 aagctgcgac aggccaaact gctccgcccg gtcaacgatc agcgtgttgg cattgggaaa
     3841 gtccaggccg ctttcgatga tggtcgtgct caggaggatg tccacccgcg cctcggcaaa
     3901 ctcgaccatg atcttctcca gctgccgctc actcatctgc ccgtgagcga cgacgatgcg
     3961 cgcctccggg gccagcttct ccagccgttt gtgaatgatg tggatgctct ggacgcggtt
     4021 gtggacgaag aagacctggc cgccccggtc tagctcacgc aggatggccg tgcggatgcg
     4081 ggcgtcgtcg gattcgccga cgtaggtctc cacgggcagg cgctcggccg gggcggtgtc
     4141 gatgatgctg atgtcccgga caccggtcag ggccatgtgc agcgtgcgcg ggatgggcgt
     4201 tgccgtcagt gtcagcacat cgacctcggt gcgccactgc ttcagcttct ccttgtcggc
     4261 cacgccaaag cgctgttcct catcgatgat gaccagcccc aggtccttga aggcgatatc
     4321 gtcggacagc aggcgatggg tgccgatgat gatatcgact tgcccggcgc gcacctgctg
     4381 tacgatgcgt cgctgctgcg tggccgtgcg gaagcgcgac agcatctcga ccgtgaccgg
     4441 gaagggcgtc agacgttcgc ggaaggtggt gaagtgttgc tgggccagga tggtcgtcgg
     4501 caccagcatg gccacctgct tgccatcgtt gaccgccttg aaggccgcgc ggagggcgac
     4561 ctcggtcttg ccatagccga catcgccgca gatgaggcgg tccatcggat tggcgcgctc
     4621 catgtcgtcc ttgacctcgc cgatgacgcg cagctggtcc tccgtctcgc ggtaggggaa
     4681 ggcggcctcc agctcggcct gccactcgga gtcgggcggg aaggcatggc cgggcacggt
     4741 ctcgcgggcg gcgtagaggt caagcaactc ctgggctaat tcgtcggccg cccgttgggc
     4801 ggcggccctg gcctgtgtcc aggtgcgctc gcccagccgg ttgagcgatg ggtcggcgtc
     4861 gtccgggccg atccacttgc ccaggcggtc ggcgtggtgg acgggcacgt agagcgtgtc
     4921 ggcgttggcg tactgcacca gcaggtactc gcgctccatg ccgcccagat tgcgcaccac
     4981 cagcccctca aacttgccga tgccgtagtc gagatggacg acatagtcgc cctgcttgat
     5041 gtcggcgaag taggcttctg gcgcggccgg gcgggccgtg cgctggcggc gcggcgcggg
     5101 gcggctccag ccgaagatct cggcatcggt caacaggtgg agcagcacgc ggtgatcttc
     5161 gcgccgcacc aggctgaagc cgtcggccag gctgccgtgg acgaaggtca agctgccgtc
     5221 cgggggcgct tccggcaggg agcttagcga gaccgtgccc gcgcggccag tgccggcgtc
     5281 gatggcggtg acgacgggat cgacggcgtc ggacggcccc gcctgggtca gtggctccag
     5341 cgtacggatg agcttgccgc cctcgccggc ccatagctcg gccaggcgcg gggcctgccg
     5401 gct
//
LOCUS       DAOSMQ010000197         5379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15342_length_5379_cov_4.311232, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000197 DAOSMQ010000000
VERSION     DAOSMQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15342_length_5379_cov_4.311232"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..576
                     /locus_tag="PK829_07805"
     CDS             121..576
                     /locus_tag="PK829_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOU41158.1"
                     /translation="MATKKAATRDEDVQSFTKEERAAMKERAAEMKAAARHGAKREDG
                     ERDVLAKIAEMPEPDRSLAERVHAIVTATAPELIPKTWYGMPAYTRDGKVVCFFQAAE
                     KFGARYATLGFNDVAKLDEGAMWPTAFALIELTAAEEARIAALVQRAVS"
     gene            complement(647..1477)
                     /locus_tag="PK829_07810"
     CDS             complement(647..1477)
                     /locus_tag="PK829_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU41159.1"
                     /translation="MTLIITSPTNPRVKSVVRLADRRTRDELRRTVVEGVREVGLALG
                     RGVAPVEAYVCPELIAGAEAEAAARHLVALGGAGMTELFYVAPAVFAKMAYRGQSGGL
                     LLVIAYPRRTLDELTFRAAPFLVVVEEAEKPGNLGAVLRTADAAGVDALLVPTLPGQT
                     GTDLHNPNVIRASLGAYFTVPVVTSAAEEAIAWLRARGIAIVATTPATETVYTAADLT
                     GPVAVVMGSEAWGLSETWLAAADMRVRIPMAGQVYSLNLSAATALVVYEVVRQRGRGE
                     "
     gene            complement(1506..1904)
                     /locus_tag="PK829_07815"
     CDS             complement(1506..1904)
                     /locus_tag="PK829_07815"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOU41160.1"
                     /translation="MRQEVARRANHCCSYCRSQEQVAGTLFTVDHIIPQALGGSDDLD
                     NLCLACWDCNRIKQAHVTGVDPSLGKRVTLFHPQRQRWPDHFCLGSRRRGGRWNDRGW
                     ASNCRATATEPRFPRPCAQAMDSSWVASSR"
     gene            complement(1925..2278)
                     /locus_tag="PK829_07820"
     CDS             complement(1925..2278)
                     /locus_tag="PK829_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41161.1"
                     /translation="MDTKSLPELVQQLPLDAQEVVRDLVELLLARPKAEATPQSPRFL
                     LQAARMRVPEDRAKRMQELVWKQQSEGLTTVEEDEAAQLQQFARRVMLVRADAAALLA
                     ARGHDVAGLISQAVP"
     gene            complement(2373..2798)
                     /locus_tag="PK829_07825"
     CDS             complement(2373..2798)
                     /locus_tag="PK829_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOU41162.1"
                     /translation="MPTLLPNRIPRAARIGEKGAFTRTLTEADAALFIGVTWDLNPYH
                     TDDRYVAQTPFGRRIVPGLLTASLLTHLGGLWAFLATEMTFEFLGPVYIGDTVTAEAE
                     VVEVSERGWVRLAGRVVAADGREVLRASIRGYPGRFEEE"
     gene            2951..3271
                     /locus_tag="PK829_07830"
     CDS             2951..3271
                     /locus_tag="PK829_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU41163.1"
                     /translation="MTRDSTFEQTTAEDIRRTLQAHRQTLGGRFGVRSLALFGSVARR
                     EATAESDIDLLVQFDQPIGLFELFALQDELEAILGRPVDLGTINSLKPRVRERVLEQA
                     VDVF"
     gene            3261..3632
                     /locus_tag="PK829_07835"
     CDS             3261..3632
                     /locus_tag="PK829_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOU41164.1"
                     /translation="MSSREWPERIRDILDAIVETQTFTEGMSLEDFKADAKTVKAVEL
                     DFIIIGEAARAVPEAVQKANSAVPWQIMRSMRNRLVHAYFHIDPDILWKTIKDELPLL
                     VPRLEEMLNELPGEDEQNETG"
     gene            complement(3659..>5379)
                     /locus_tag="PK829_07840"
     CDS             complement(3659..>5379)
                     /locus_tag="PK829_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HOU41165.1"
                     /translation="LIRHHLLDNPHRVTVVLAPDAELARRQSEAEAERLAAARAAMSA
                     EEAAAVMAQAAELKRQQETPDPPEVVALLPSLRRADLEPRHKPIPLAEHELAGARVLS
                     HDLFTNGIVYLDLALDVQPLPAELLPYVPLFGRALTQMGTAAEDFVRLSQRIGQTTGG
                     LYATTLVAPVRREAEQGSKGAGEQGREARTGDASSSPAQWASSATAAHLLLRGKATAA
                     RAPELLAILRDVLLTVRLDNRDRFRQILLESKARLEAQLAPAGHVLANTRLRAHFHPA
                     LRVEETFGGLSQLLFLRQLAEQVEADWPGVLARLEAVRAALITRAGALANVTLDAAGY
                     AAFAPRLEELLAALPLGDDGRQTMDDSQSGDRGPSSVVRGLSFPAHEGFAFPSAVNYV
                     AKGANLYDLGYAYHGSIAVINNLLRNDYLLPKVRIQGGAYGAFATFSPNAGVLTFLSY
                     RDPNLANTLRAYDGAAGFLRGLALDEAALAPAIVGAIGSIDAYQLPDAKGYTSLVRYL
                     TNETDAYLQTVRDQVLATTAADFHRLADVVAQVAAVGDVVVLGSAEALAGAAAEGVGL
                     AVVEVM"
BASE COUNT          948 a         1769 c         1782 g          880 t
ORIGIN      
        1 cttccgaatc gcatctttag ccgggctgtg gcgcgattct tccgaatcgc atcttcaaag
       61 gacagcttaa gtttagaacg cgattcggag aatcgcgcca caacatcaaa ggagagtgag
      121 atggctacaa agaaggcagc cacgcgcgac gaggacgtac agagctttac gaaggaggag
      181 cgcgcggcca tgaaggagcg cgccgcggag atgaaggcgg cggcgcgcca cggcgcgaaa
      241 cgggaggacg gcgaacgcga cgtgctggcc aagatcgccg agatgccgga accggatcgc
      301 tcgctggccg agagggttca tgcgattgtc accgccaccg cgccggagct tatacctaaa
      361 acgtggtatg ggatgcctgc ttacaccaga gacggcaagg tcgtctgctt cttccaggcc
      421 gcggagaagt tcggtgcgag gtacgcgacg ttgggcttca acgacgtggc caagctcgac
      481 gagggcgcca tgtggccgac cgctttcgcg ctgatcgaat tgacggcggc cgaggaggcg
      541 cggatcgcgg cgcttgtgca gcgggcggtg agctagagca agagcgccgc aaccggtttc
      601 atgtgccggc cgccggttgg aaaaccagcg gccggcacag gccgtcctac tctccccgcc
      661 ccctttgccg caccacctca tacaccacca acgccgtcgc cgccgataaa ttcaggctat
      721 acacttgccc ggccatgggg atgcgcacgc gcatgtcggc cgccgccaac cacgtctcgc
      781 ttagccccca cgcctcgctg cccatgacca cggccaccgg gccggtcagg tcggcggccg
      841 tgtaaacggt ctcggttgcc ggcgtcgtgg ccacgatggc aatgccgcgc gctcgcagcc
      901 aggcgatggc ctcctcggcc gcggacgtga cgacgggtac ggtgaagtac gctcccaggc
      961 tggcgcgaat gacgttgggg ttgtggaggt ctgtgcctgt ctgccccggc agggtgggca
     1021 cgagcagcgc gtccacgccg gcggcgtcgg ccgtgcgcag gaccgcgccc aggttgcccg
     1081 gtttttccgc ctcttcgaca acgacgagga agggcgcggc gcgaaaggtc agctcgtcta
     1141 gcgtccgccg cgggtaggcg atgaccagca gcagcccgcc gctctggccg cgataggcca
     1201 ttttggcgaa cacggccggc gcgacgtaga acagttctgt catgcccgcc ccgcccaggg
     1261 cgacgagatg acgcgccgcc gcctcggcct ccgccccggc gatcagttcc gggcagacgt
     1321 aggcctccac cggggcgacg ccgcggccga gggccagccc cacctcgcgc acgccctcga
     1381 cgacggtgcg gcgcagttcg tcgcgcgtcc gccggtcggc cagcctgacg acggacttga
     1441 cgcgggggtt ggtggggctg gtgataatta aggtcatccg caacctctac ggcgcgtcgc
     1501 tcgtgtcaac gggaggatgc cacccaactc gaatccattg cctgcgcgca cggacgagga
     1561 aagcgcggtt cagttgcagt tgctcgacag ttgctcgccc aacctctgtc gttccaacga
     1621 ccaccgcgcc gtcttgatcc caggcaaaaa tgatccggcc aacgctgccg ttgcgggtgg
     1681 aatagagtca ctcgcttacc caatgaggga tcgacaccgg ttacgtgggc ttgcttaatg
     1741 cggttgcaat cccagcaagc cagacacaag ttgtccaggt catcgctgcc gccgagcgcc
     1801 tgaggaatga tgtgatccac cgtaaacagc gtgccggcaa cctgctcttg gctacggcaa
     1861 tagctgcaac aatgattggc gcggcgggcc acttcctgac gcattgccgc gctcagcggc
     1921 tttgtcacgg aacagcctga gaaatcaacc cggcaacatc gtgacctcgc gcggcaagaa
     1981 gagctgcggc gtcggcgcga accagcatga cccggcgcgc gaactgctgt agctgcgccg
     2041 cctcgtcttc ctcgaccgtc gtcaaccctt ctgactgctg cttccagacc aactcctgca
     2101 tgcgcttagc cctgtcctcc gggacgcgca tacgagcagc ctgaagcagg aagcggggcg
     2161 actgtggcgt agcttctgcc ttaggccgtg ccagcaacaa ttcaaccaga tcgcgcacga
     2221 cttcctgcgc gtccagaggc agttgctgca caagttccgg taagcttttg gtatccatag
     2281 gcgacgctct cttctacatt cagtatacta tgagatgcgc gagtagggtg tttgcgctcg
     2341 gttgcccaca aaccaacgcg gaagattggc gatcattcct cctcaaaccg ccccggatag
     2401 ccgcggatgc tggcccgcag cacctcgcgg ccgtcggcgg ccacgacccg cccggccagc
     2461 cgcacccagc cgcgctcgct gacctccacg acctccgcct cggccgtcac cgtgtcgccg
     2521 atgtagaccg ggccgaggaa ctcaaaggtc atctccgtgg ccaggaaggc ccacaggccg
     2581 ccgagatggg tcaacaggct ggccgtcagc aggccgggga cgatgcgccg gccgaatggc
     2641 gtctgggcca cgtagcggtc gtcggtgtgg taggggttga ggtcccaggt gacgccgatg
     2701 aacagggccg cgtcggcctc ggtcagcgtg cgggtgaaag cgcctttctc gccaatgcgg
     2761 gcggcgcggg gaatgcggtt ggggaggagg gtgggcatgg gcacattata gacaaggacg
     2821 ctcgcgcgca caattcgccc gcagagcaat cgcccatacg ggacgagcga cgtgtagccc
     2881 ctggcgtcgg gcagcaggta ggcgtcgatc gcgcccagat cgtagctgaa tatgctatac
     2941 tgtgctcgtc atgacccgcg attcgacttt tgagcagaca acggctgagg acatcaggcg
     3001 gacattgcag gcccacaggc aaacccttgg cggtcgcttc ggtgttcgct ccctggcgct
     3061 gttcggctct gtcgcccgcc gcgaagcgac ggctgagagc gacatcgacc tgttggttca
     3121 atttgaccag ccgatcgggc tatttgagct gtttgccctg caagacgaac tcgaggcgat
     3181 tttgggccgg ccggttgatc tgggcaccat caactccctc aaaccgcgcg ttcgcgagcg
     3241 ggtgctagag caagctgtcg atgtcttcta gggaatggcc cgagcgcatt cgcgatattc
     3301 ttgatgcgat cgtggagaca cagaccttca cggaagggat gtctctagaa gactttaaag
     3361 ccgatgcgaa aactgtcaag gccgtagaac ttgactttat catcattggc gaagcagccc
     3421 gcgccgtgcc cgaagctgtc caaaaggcta actcggccgt tccatggcaa atcatgcgct
     3481 ccatgcgcaa tcgattagtt catgcctact ttcacatcga tccagacatt ttgtggaaga
     3541 ccatcaagga cgaactgcca ttgctggtcc cacgcctgga agaaatgctg aatgagctgc
     3601 caggcgaaga tgaacagaat gaaaccgggt gatgcctgct gaaagcatga tcgcccctct
     3661 acatcacctc caccaccgcc aaccccaccc cctcggctgc cgcccccgcc agcgcttccg
     3721 cgctacccag cacgaccaca tcgcccaccg ccgccacctg ggcgacgacg tcggccaggc
     3781 ggtggaagtc cgcggccgtc gtcgccagca cctggtcgcg gacggtctgc aaataggcgt
     3841 cggtctcatt ggtcagatag cggacgagcg acgtgtagcc cttggcgtcg ggcagctggt
     3901 aggcgtcgat ggagccgatg gccccgacga tggccggggc cagcgccgcc tcgtccagcg
     3961 ccaggccgcg caggaagccg gccgcgccgt cgtaggcccg cagggtgttg gccaggttcg
     4021 ggtcgcggta ggacaggaag gtcagcacgc cggcgttggg gctgaaggtg gcaaacgcgc
     4081 cgtaggcccc gccctggatg cggaccttcg gcaacaggta gtcgttgcgg agcaggttgt
     4141 tgatgacggc gatggagccg tggtaggcgt agcccaggtc gtagagattg gcccccttgg
     4201 ccacgtagtt gaccgccgag gggaaggcga agccctcatg cgcggggaag gagagaccac
     4261 ggacgacgga cgacggccca cggtctccgc tctgactgtc gtccattgtc tgtcgtccgt
     4321 cgtcccccaa cggcaacgcc gccagcaact cctctagccg cggcgcgaac gccgcatagc
     4381 cggccgcgtc gagggtcacg ttggccaacg ctccggcacg cgtgatcagc gccgcgcgca
     4441 ccgcctccag ccgggccagc acgccgggcc agtccgcttc gacctgctcg gccagttgcc
     4501 gcaagaagag cagttggctg aggccgccga acgtctcctc gacgcgcaac gccgggtgga
     4561 agtgggcgcg caggcgggtg ttggccagca cgtggccggc cggggccagt tgcgcctcca
     4621 gccgcgcctt gctttccagc aggatttgcc ggaagcggtc gcggttgtcc agccggacgg
     4681 tcagcagcac gtcgcgcagg atggccagca gctccggggc gcgggcggcc gtcgccttgc
     4741 cgcgcagaag cagatgggcg gccgtggcgc tggacgccca ttgggccggg gaggacgaag
     4801 catcgcccgt tctcgcttct ctcccctgct cccccgcgcc cttgctcccc tgctcggcct
     4861 ctcgccgcac cggcgcgacc agcgtcgtcg cgtacagccc gcccgtcgtc tggccgatgc
     4921 gctgcgacag gcgcacgaag tcctcggccg ccgtacccat ctgcgtcagg gcgcggccga
     4981 agaggggcac atagggcaat agctcggccg gcagcggctg cacgtcgagg gccaggtcga
     5041 ggtagacgat gccgttggtg aacaggtcgt ggctcagcac gcgcgccccg gccagttcgt
     5101 gctcggccag gggaatgggc ttgtggcgcg gctccaggtc ggcgcggcgc aggctgggca
     5161 gcagggcgac gacctccggc gggtcgggtg tctcctgctg gcgtttcaat tcggccgcct
     5221 gggccataac cgcggcggct tcctcggccg acatcgccgc ccgggccgcg gccaaccgct
     5281 cggcctcggc ctcgctctgc cgccgggcca gctccgcgtc cggcgccagc accacagtga
     5341 cgcgatgggg gttgtcgagc aggtggtggc ggatgaggc
//
LOCUS       DAOSMQ010000198         5373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15362_length_5373_cov_4.578789, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000198 DAOSMQ010000000
VERSION     DAOSMQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15362_length_5373_cov_4.578789"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PK829_07845"
     CDS             <1..1165
                     /locus_tag="PK829_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOU41166.1"
                     /translation="RWQIRDADGVAFGHLYAEITVVAAPPPPNARRTGMTFLRDQTVP
                     DGTPYVAGADFYKQWAVRNSGERNWGSGFRLVFAQGDVQMARGVTAHVVPDAQPGDEV
                     ILSVPMTAPATIDGKPTAYSSLWRMQDDRGAFFGDPIWAKITTTPGVGTPGVGTPGGA
                     TPGGATAGVVAVDGPLSVGTALSRLVNDPSAWYSQLDARWAKVAVGNGQQRIESWGCL
                     MTCMAMAMSAYGLRLTPLELNDKLKAQGDNGFRGSNVQFIGPTYVLAGLRQGANLRSY
                     ESPALDATTWTGEDPLARIDRNLAAGAIVLAQVDTRPNDGLYNSNIEQHWVILVKRTP
                     AGEDYLILDPVVPPDQVRDQPRSLMLKYGDRKPNRSNNDNLRQAIKSTLVYYM"
     gene            1413..1499
                     /locus_tag="PK829_07850"
     tRNA            1413..1499
                     /locus_tag="PK829_07850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1447..1449,aa:Ser,seq:tga)
     gene            1540..1629
                     /locus_tag="PK829_07855"
     tRNA            1540..1629
                     /locus_tag="PK829_07855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1574..1576,aa:Ser,seq:gct)
     gene            1720..1793
                     /locus_tag="PK829_07860"
     tRNA            1720..1793
                     /locus_tag="PK829_07860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1754..1756,aa:Arg,seq:acg)
     gene            1897..2241
                     /locus_tag="PK829_07865"
     CDS             1897..2241
                     /locus_tag="PK829_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41167.1"
                     /translation="MRTPAGKECRFYYQDFFRGRSDQECRLIKANPRSPQWKPSDCAN
                     CPVPDILMANSNPNLVLEATVKPGVLGIGRHVTVKAFCSRHLIDVEQPEVGCPQCAAE
                     RPGLRELFNDLH"
     gene            2250..2996
                     /locus_tag="PK829_07870"
     CDS             2250..2996
                     /locus_tag="PK829_07870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU41168.1"
                     /translation="MNAPIQAVLLDLDDTLLGNNTAEFMKRYFALLGAYARPVMDNDT
                     FLPHLIQATQATIRNTDPTMTNAEVFWANFEGLTGGRRSELEPFFNRFYESEFARLQP
                     STLVRPAAVHLVRAAFARGLAVVIATNPLFPATAIEQRLAWAGVPIDTYPYALVTTYE
                     NMHAAKPQPAYYREILAAIDCPPDRALMVGDDWKNDIAPAAALGLYTYWIAPIDAEPP
                     DATLLNGQGSLDVLAELVAGDWLERLGRPA"
     gene            3004..3501
                     /locus_tag="PK829_07875"
     CDS             3004..3501
                     /locus_tag="PK829_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU41169.1"
                     /translation="MLPARTLLNPPPTGELLAELAAFRERIIGLLADGAADWSRRPAD
                     AEWSLTEIACHLRDVEREVHQARFQALLEDSSAFLPGVDADEWAITRHYRAQDGRAAA
                     AAFVAARAETITRLAPLPPEVWETQGQHTFFGPTSLHELVHLAVQHDRVHGQQIDMLR
                     DGSAE"
     gene            complement(3514..4494)
                     /locus_tag="PK829_07880"
     CDS             complement(3514..4494)
                     /locus_tag="PK829_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOU41170.1"
                     /translation="MSGQVWLISLLAAAVPVLFYVWLIYWVDRYEKEPWWLLAAAFLW
                     GAIPAALLALIFNTALSLPLYALLNPGPAELLSGGFFAPIIEEIAKGLILILIFVLWR
                     QELDSPLDGLVYGALVGMGFAMVENVLYYQSAFAAGGQTVWNSTVLIRGVIFGLSHAF
                     YTSLTGLGIALARLSPSRSVRILAPLLGLGAAIFFHALHNVTMFGGTALSFLAGLTFD
                     WGGVLLTVVIIVLVLARERQWMQHYLADEVTQGTLTADEYTLVRSAAARNRRRLGLLL
                     SDGPNAWRRSGRRYRECAELAYLKRHHAIFGDERSHAAAERLRETLRAGI"
     gene            4580..5233
                     /locus_tag="PK829_07885"
     CDS             4580..5233
                     /locus_tag="PK829_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41171.1"
                     /translation="MTEDHDPEVLMGTSNAAVTTARRLIINMALARAQPGAGSSPSFM
                     HRRTAMQHFPDLRDILQGIDWLAVGAVAARAYMPERATQDLDILVRAAEADRVAARLE
                     AAGYVHTAELGIPGAAFARTGEPEVDVLFGDQPWLEEALAGPGSDPAGLPVLALPYLI
                     LMKLTAGRARDIADVATMLGWADETALEAVRDVIDRYSPADAPDLESLIYLGKRERE"
     gene            complement(5240..5371)
                     /locus_tag="PK829_07890"
     CDS             complement(5240..5371)
                     /locus_tag="PK829_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU41172.1"
                     /translation="MAANIVAYREANGPVATIEAIMDVPGIGEGRFAEMKDLITVGP"
BASE COUNT          952 a         1831 c         1713 g          877 t
ORIGIN      
        1 ccgctggcag atccgcgacg ccgacggtgt ggccttcggc cacctctatg ccgagatcac
       61 cgtcgtggcc gcgccgccgc cgcccaacgc tcgccgcacg ggtatgactt tcctgcgcga
      121 ccagaccgtg cccgacggca cgccctatgt cgccggcgcg gacttctaca agcagtgggc
      181 cgttcgcaac agcggcgagc gcaactgggg cagtggcttc cggctggtct ttgcccaggg
      241 agatgtgcaa atggcccgcg gcgtgaccgc ccacgtcgtc cccgacgccc agccgggcga
      301 cgaggtcatc ctgtccgtgc ccatgaccgc gcccgcgacc atcgatggca agccgacggc
      361 ctatagcagc ctgtggcgca tgcaggacga ccgcggcgcg ttcttcggcg acccgatctg
      421 ggccaagatc accaccacac ccggtgtggg cacacccggt gtaggcacac ccggcggggc
      481 cacgccgggc ggagccacgg ccggcgtagt cgcggtcgat gggccactgt cggtcggcac
      541 ggcgctcagc cgtctggtga atgacccgtc ggcctggtac tcgcagctcg acgcgcgttg
      601 ggccaaggtc gccgtcggca acggtcagca gcgcatcgag tcgtggggct gcctgatgac
      661 ctgcatggcc atggccatga gcgcctacgg cttgcgtctg accccgctgg agttgaacga
      721 caagctaaag gcgcagggcg acaacggctt ccgcggttcc aacgtgcagt tcatcggccc
      781 gacctacgtc ctggccgggc tgcggcaggg ggccaatctg cgctcctacg aaagcccggc
      841 cctcgacgcg acgacgtgga cgggcgaaga cccgctggca cgcatcgacc gcaatctggc
      901 cgccggggct atcgtcctgg cccaggtgga cacgcggccc aacgacggcc tctacaactc
      961 caacatcgag cagcactggg ttatccttgt caagcgcacg ccggccggtg aagactatct
     1021 catcctcgat ccggttgtgc cccccgacca ggtgcgcgac cagccgcgct cgctgatgct
     1081 gaagtacggc gaccgcaagc ccaaccgcag caacaacgac aacctgcgcc aggcgatcaa
     1141 atcgacgctg gtctattata tgtagggcca cttctggccc tcaggatcag ggccaagtcg
     1201 gaagaagacc tcgcgcaaag atcgccaagg acgcaaagaa cgccaataag aaactctctt
     1261 ggcgttcttt gtgctacctg gctatctttg cgtgagggtc ttcttggcgt ctttgccgct
     1321 ttgcggcttg gcgtgagctc ttcgccgtcg gtggttgccc acgcgggcgg ggcatgctat
     1381 actttagcga cgttccctcg ccggaaaacc gcggagggat ggcagagcgg acgattgcag
     1441 cggtcttgaa aaccgcagtg ggcgcaagtt caccgggggt tcgaatccct ctccctccgc
     1501 ctgaggttga caaccgagcg cgcaaacacg ccttcgcatg gggaggtgcc agagtggtcg
     1561 attggggccg cctgctaagc ggttaccggg cttaaaactc ggtcatgggt tcgaatccca
     1621 tcctccccgc ttctgcctcg ggcatgaggt caatgagcaa gtcgcgcggg cgcttgccaa
     1681 gcgagcgcgt ttcggctatg cttacgaacg ttgacagacg cgcccttagc tcagtggatt
     1741 agagcgcatg gctacgaacc atgaggccgg gggttcgaat ccctcagggc gtactgagtg
     1801 tccgcaagcc ctatatcgag cgatatgggg cttttctttt gttgccgacc ggctataacc
     1861 ggtaggcaat gtgggacgta gggagttatt cgccgcatga ggacccccgc cggcaaagag
     1921 tgccgcttct actatcagga cttcttccgc ggccgatccg atcaggagtg ccggctgatc
     1981 aaggccaacc ctcgctcgcc gcaatggaag ccgtctgact gcgccaactg ccccgtgccc
     2041 gacatcttaa tggctaacag caatcccaac ctcgtgctgg aagcgaccgt caagcccggc
     2101 gtgctgggca tcggccgcca tgtcaccgtt aaggcctttt gcagccgcca tctgatcgac
     2161 gtggagcagc cggaggtcgg ctgcccccaa tgcgccgccg agcggccggg cctgcgcgag
     2221 ttgttcaacg atctccacta gatgccgcca tgaacgcacc tatccaggcc gtccttctcg
     2281 atctcgacga taccctgctc ggcaacaaca cagccgagtt catgaagcgc tactttgccc
     2341 tgctgggcgc gtatgcgcgc ccggtgatgg ataacgacac cttcctgccc cacctcatcc
     2401 aggcgacgca ggccaccatc cgcaacaccg acccgaccat gaccaacgct gaagtcttct
     2461 gggccaactt cgagggcctg acgggcggcc ggcgcagcga actggagccg ttcttcaacc
     2521 gcttctacga gagcgaattc gcccggctgc aaccgtccac gcttgtccgc ccggcggccg
     2581 tccatctggt gcgggccgcc tttgcgcggg ggctggccgt cgtcatcgcc acgaacccgc
     2641 tcttcccggc cacggccatc gagcagcggc tggcctgggc cggcgtgccc atcgacacgt
     2701 acccctacgc gctcgtgacc acctacgaga atatgcacgc cgccaagccg cagccagcct
     2761 actaccgcga aatcctggcc gccatcgact gcccgcccga ccgtgccctc atggtcggcg
     2821 acgactggaa gaacgacatt gccccggcgg cggcgctggg cctgtacacc tactggatcg
     2881 cacccatcga cgccgaaccg cccgacgcga cgctgctcaa cggccagggg tcgctcgatg
     2941 tgttggccga gttggtggcc ggcgactggc tggagcgcct cggccgcccg gcctgaactg
     3001 tctatgctac ccgcgcgcac cctcctcaac ccgccgccca ccggcgaact gctggccgaa
     3061 ctggccgcct ttcgcgagcg catcatcggg ctgctggccg acggggcggc cgattggtcg
     3121 cgccgcccgg ccgacgcgga gtggtcgctg acggagatcg cctgccacct gcgcgacgtg
     3181 gagcgcgagg tgcatcaggc gcgcttccag gccctgttgg aggacagcag cgccttcctg
     3241 ccgggcgtcg acgcggacga gtgggcaatt acgcgccact accgcgccca ggacggccgt
     3301 gccgccgcgg ccgcgtttgt cgccgcccgc gccgagacca ttacccgact ggctcccctg
     3361 ccgcccgaag tctgggagac ccaggggcag catacctttt tcggtccgac ctcgctgcac
     3421 gagttggttc acctggccgt gcagcacgac cgcgtccacg gccagcagat cgatatgctc
     3481 agggacggca gcgccgagta gccccagcgg gcgctagatg cccgcgcgca acgtctcgcg
     3541 caggcgctcg gccgccgcgt ggctacgctc gtcgccgaag atggcgtggt ggcgtttgag
     3601 ataggccagt tcggcgcact cacggtagcg ccggccgctg cgccgccagg cattggggcc
     3661 gtcgctcagc agtagcccca gccgccgccg gttgcgggcc gcggccgagc gcaccagggt
     3721 gtactcgtcg gccgtcaacg tcccctgcgt cacttcgtcg gccaggtagt gctgcatcca
     3781 ctgccgctcg cgggccaaca ccaaaacgat gatgaccact gtcagcagca ccccgcccca
     3841 gtcgaacgtc agcccggcca ggaagcttaa tgccgtgccg ccgaacatgg tcacgttgtg
     3901 cagggcgtgg aaaaagatgg ccgcgcccag acccagcagc ggggctagaa tgcgcacgga
     3961 gcggctggga ctgagccggg ccagagcgat gcccaggccg gtcagcgacg tgtaaaaggc
     4021 gtggctgagg ccgaagatga caccgcgaat gaggactgtg ctgttccaga ccgtctgacc
     4081 gccggcggcg aaggccgatt gatagtagag aacgttttcc accatggcga agcccatgcc
     4141 caccagcgcg ccgtagacaa gaccgtccag tgggctatcc aactcttgcc gccacaggac
     4201 gaagatgagg atgaggatga gacccttggc gatctcctcg atgatcggcg caaagaagcc
     4261 gccggagagc agttcggccg ggccggggtt gagcagcgca tagagcggca gactgagagc
     4321 cgtgttaaag atcagcgcca gcagcgccgc ggggatggct ccccacagga acgccgccgc
     4381 caacagccac cacggctcct tctcgtagcg gtcaacccag tagatgagcc agacgtagaa
     4441 cagcactggc acggccgcgg ccaacagcga tatgagccat acctgtccgc tcatgcgccc
     4501 tcgccctttt gtccatctgc tataatggct gtcatgaggt cgaattatac atggcttcag
     4561 ccgtaaacga ggcgcgattg tgaccgaaga ccacgatccc gaagtcctca tgggcacgtc
     4621 caacgccgct gtgaccacgg cgcgacggct gattatcaat atggccctgg cgcgcgccca
     4681 acccggcgcg ggcagcagcc catcgttcat gcacaggagg acggcaatgc agcacttccc
     4741 cgatttgcgc gatatcctgc aagggatcga ctggctggcc gtcggcgcgg tggcggcgcg
     4801 cgcctatatg cccgagcggg cgacgcaaga cctcgacatc cttgtgcgcg ccgccgaggc
     4861 tgaccgcgtg gccgcgcggc tggaggcggc cggctatgtt cacacggccg aattgggcat
     4921 ccccggcgcg gcctttgcgc ggacaggcga gccggaggtc gatgtgctct ttggcgatca
     4981 accctggctg gaggaagctc tggccgggcc aggcagcgac ccggccggct tgcccgtgtt
     5041 ggctctccct tacctgatac tgatgaaatt gacggcgggg cgcgcgcgcg acattgccga
     5101 tgtggccaca atgctcggct gggcagacga gacggcgcta gaggccgtgc gcgacgtgat
     5161 cgaccgctat agcccggccg acgcgcccga cctggaatcg cttatctatt tgggcaaacg
     5221 cgaacgggaa tagacgcggc tacggcccaa ctgtaatcag gtccttcatc tccgcaaagc
     5281 gcccctcgcc gatgccgggc acgtccatga tagcctcgat ggtagcgacg gggccgttgg
     5341 cctcgcggta ggcgacgatg ttggccgcca tcg
//
LOCUS       DAOSMQ010000199         5336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15502_length_5336_cov_3.455596, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000199 DAOSMQ010000000
VERSION     DAOSMQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15502_length_5336_cov_3.455596"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PK829_07895"
     CDS             <1..578
                     /locus_tag="PK829_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU41173.1"
                     /translation="SELPAHLTYHSLHHTRDDVLPACLRLADVSGIPEADVLCLATAA
                     AYHDIGFLYSYAEHEAHGIALARAVLPGFGYSDSQIDTISALIAATRMPQTPTTPLAQ
                     LLCDADLDVLGRDDFWDLNRKLLAETARYGRNGTVSEEEWLGEQMRFLQRHVYFSTAA
                     RRLRDDGKARNMARMQRAIAAGDSEESRHAH"
     gene            complement(575..1246)
                     /locus_tag="PK829_07900"
     CDS             complement(575..1246)
                     /locus_tag="PK829_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOU41174.1"
                     /translation="MALTRERMGEWINEHYTLGEEIAHSVTHGVGAVLSGAGLVVLLV
                     LAIGRGSPWHILSFAVYGVSLLALFLASTLYHGVQKPRLRPILRKVDHACIYLLIAGT
                     YTPFVLVAMRGALGLTLLAVVWALAVFGIVYKMFFIDRFVVITTLAYVAMGWMSVIAW
                     KQMVANIPPVGLIFLMAGGGLYTIGVIFYAMTKIRYTHAVWHVFILGASICHFVAVLT
                     LLRLA"
     gene            complement(1413..2564)
                     /locus_tag="PK829_07905"
     CDS             complement(1413..2564)
                     /locus_tag="PK829_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41175.1"
                     /translation="MNKFVAALLRLLGGLIGVGVVVLPLAAAPRATQEPIPYPVWTIE
                     DVALSGYDNALAVDSAGRPHLLYVAPANGALRYAVRQSDGWHYEDVADESADLPLLAY
                     DLAIRPDGAVCLVYATELSIPSQPFDTKLVYGCRGAHGWELSTIDDGGRAPRLRFDDA
                     GQPHIALIQDNIYVVYLTWADGRWQKDTVYTDSAYLNVVFLLLDSAGHPSVVFRGADG
                     QFHAQRSESGSWASTPLTRLSIYTAALDANDALWLAVNDGESLPGHDPFFLARLKLVR
                     PADSGPDDWELLDEGYDWQIAADMAFSDGIPYLAYIDPDGAAQVVWWDESGKHGDMAG
                     AQGFSELSLAMHDGQPRLAFADDNQLKLASRDIILLDKVLFAPVIVGAP"
     gene            complement(2709..4790)
                     /locus_tag="PK829_07910"
     CDS             complement(2709..4790)
                     /locus_tag="PK829_07910"
                     /inference="COORDINATES: protein motif:HMM:NF039734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HOU41176.1"
                     /translation="MHGNGWSIRRLLAVCLALLSLMLSMAFVSGAEQERPQAPAGADA
                     TLDDGGTAEFGIEWITDWPGTADDRPNWYHSANGLRAELVDGGWLQRFFWGNSLAWEE
                     DFKGAASGGTEHIWIDTVDLGLMATHGSGTWDSFWSKNLSSVYYSTNHDDLHLSPGEA
                     YHAFGDDDLEWLAWDSCSVLDDNSAAYWYTTFDGLHLMLGFANTMYVVYPGDGGAWGD
                     QMRAKGWWIFGHGAKTVTQAWFTATDDQQPSGVRARVLAEELDNYNDYIWGQGYVSPD
                     PSYNGLYWYWDHVAGTPPPAQITTEFQELPVFLIRPREVNEDYIRNIGQSFGLDSEIL
                     AAPDGSAFYMVGGEGDAKQVRIDARTGAFYYQDLSELWTEPERPRALPDSAERAAGLA
                     HSFLAAHDNLPGVFEFNGQIPPTVYLESASEGASPETADLRRPLASNPMQYSVSYMRT
                     VDVGGTPLSIVGPGSRQNVYVGDGGEVIGLKSGYVPVEVSPARASVPILTAAEAWDAF
                     LADPSVAVAQPPTADTYKLTDTPPTLAYYQQPTTEAQQELIPVWVFEADLYVTTPDGR
                     SATAEHLLADNALIYVRAERGEDAGPTAKIDTPVAGVTLRPWETIDLSGSASGGTPPY
                     TFEWSSEVNGTLGQGETLNDVALSADARAASYGPNRVILTVTDANGLSAIDEVQIGVL
                     VGNYLPTVRRD"
BASE COUNT         1029 a         1728 c         1739 g          840 t
ORIGIN      
        1 caagtgagct gcccgcccac ctcacctatc actcgctcca ccacacccgc gacgacgtgc
       61 tgcccgcctg tctgcggctg gccgacgtca gcggcatccc tgaagcggac gtactctgcc
      121 tggcaacggc agctgcctac cacgacatcg gcttcctcta cagctacgcc gagcatgaag
      181 cccacggcat cgccctggcg cgcgccgtcc tgcccggctt cggctacagt gacagccaga
      241 tcgacaccat cagcgcgctt attgctgcca cccgaatgcc ccagacgccg acgacaccgc
      301 tggcccaact gctctgcgac gccgatctcg atgtcctggg gcgcgacgac ttctgggacc
      361 tgaaccgcaa gctcctagcc gagaccgcac gctacggcag gaacggcact gtctctgaag
      421 aggagtggct cggcgagcaa atgcgctttt tgcaaaggca tgtctacttc tcgaccgcgg
      481 cccgccgcct gcgcgacgac ggcaaggccc gcaacatggc ccggatgcag cgcgccatcg
      541 ccgcgggcga ctcagaagag tcgcgccacg cccactaggc caagcgcagc aacgtcaaca
      601 ccgccacgaa gtggcaaatg ctggccccca gaatgaagac gtgccagacg gcgtgggtgt
      661 agcgaatctt ggtcatggcg tagaagatca cgccgatggt gtagaggccg ccgccggcca
      721 tgaggaagat gagaccgacg ggggggatgt tggccaccat ctgcttccag gcgatcacgc
      781 tcatccagcc catggccaca taggccaggg tcgtgatgac gacaaagcgg tcgatgaaaa
      841 acatcttgta gacgatgccg aagacggcca gcgcccagac gacggccagt aatgtcagcc
      901 ccagcgcgcc gcgcatggcc acaaggacga agggggtgta ggtcccggcg atgagcaggt
      961 agatgcaggc gtggtcaacc ttgcgcagga tcggccgcag ccgcggcttc tgcacgccgt
     1021 ggtagagcgt cgaggccagg aacagcgcca gcagactgac gccgtagacg gcaaagctga
     1081 ggatgtgcca cggcgagccg cggccgatgg ccagcaccag cagcaccacc agccccgccc
     1141 cgctcagcac cgcgccgacg ccgtgggtta cgctgtgggc aatctcctcg cccagcgtgt
     1201 aatgctcgtt gatccactcg cccatgcgtt cgcgtgtcag tgccatcttg ccttccttcc
     1261 gaacggtgta gaagtgcgcc gttatataga tcaacaacac cgccggcaag aagtcgacac
     1321 ggcagatgag cgtggaagaa gagagcttag agaagcagac agacctcaaa ggtcgggccg
     1381 cggcggccgg ggcgacctct gaggtccaga atctacggag cgccaacgat caccggggca
     1441 aacagcacct tatccagcaa gatgatgtcc cgcgaggcca gcttgagctg attgtcgtcg
     1501 gcgaaggcga gccgcggctg gccgtcgtgc atggccaggc tcagctcgct aaagccctgc
     1561 gcgccggcca tgtcgccatg tttgccgctc tcatcccacc aaaccacctg cgccgcgccg
     1621 tcgggatcga tgtaggccag gtaggggatg ccgtcgctga aagccatatc ggcggcgatc
     1681 tgccagtcat agccctcgtc gagcaactcc cagtcgtccg ggccgctgtc ggccggtcgc
     1741 accagcttca gccgcgccag aaagaacggg tcgtggccgg gcagcgactc gccatcgttg
     1801 accgccagcc acagggcgtc gttcgcgtcc agcgccgcgg tatagatcga cagccgcgtc
     1861 agcggcgtgg atgcccaact acccgattca ctgcgctgag cgtggaactg cccgtccgcg
     1921 ccacggaaga cgacggacgg gtggccggcg ctgtcgagca acaggaaaac gacgttgaga
     1981 taggcgctgt cggtgtagac ggtatctttc tgccaacgac cgtcggccca tgtcaggtag
     2041 acgacgtaga tgttgtcttg gatcagggcg atgtggggct ggccggcgtc gtcaaagcgc
     2101 aggcggggcg cgcgtccacc gtcgtcgatc gtcgatagct cccagccatg cgccccacgg
     2161 cagccgtaga ccagtttcgt atcgaaaggc tggctgggaa ttgatagctc ggtggcatag
     2221 acgaggcaga ccgcgccgtc gggccgaatg gccagatcat acgccagcag aggcaggtcg
     2281 gcgctctcgt cagcgacatc ctcatagtgc cagccgtcgc tctgccgcac ggcatagcgc
     2341 aacgccccat tggcaggggc cacatagagt aggtgcggcc ggccggccga atcgaccgcc
     2401 agcgcattgt cgtagccgct aagggctacg tcctcgatag tccagacggg atagggaata
     2461 ggctcctggg tggcgcgtgg ggcggctgct aaaggcagga caacaacccc cacaccgatg
     2521 agtccgccca gcagccgcaa cagggccgcg acgaacttgt tcatacgcgc agtatagact
     2581 gcccggcgac cggacacaag ggccagccgg cccacgactg ggcgacgctc acgcgacgcg
     2641 tcgccgccgc caggcccgcg gccggccccg gctgacccta aagccagccg gggccggtcg
     2701 catggcgatt agtcccgacg gacggtcggc aggtagttac cgaccagcac gccgatctgg
     2761 acctcatcaa tggccgacag gccgttggcg tcggtcacgg tcaggatgac gcggttgggg
     2821 ccatagctgg cggcgcgcgc gtcggccgac agggccacat cgttcaacgt ctcgccctgc
     2881 cccagtgtcc cattgacttc cgacgaccac tcaaaggtgt agggcggcgt accaccgctg
     2941 gccgagccgg acaggtcgat cgtttcccag ggccgcaggg tgacgccggc gacgggcgta
     3001 tcgatcttgg ccgtcgggcc tgcgtcctcg ccgcgttcgg cgcggacgta gatgagcgcg
     3061 ttgtcggcca gcagatgctc ggctgtggcc gagcggccgt ccggcgtggt cacgtacagg
     3121 tcggcctcaa agacccacac cgggatcaac tcctgctggg cctccgtcgt cggctgctgg
     3181 tagtaggcca acgtcggcgg cgtgtcggtc agcttgtagg tatcggccgt cggcggttgg
     3241 gccacggcga ccgatggatc agccaggaag gcgtcccacg cttcggcggc ggtcaggatg
     3301 ggcacgcttg cgcgcgccgg cgagacctcc acgggcacat agcccgactt caggccgatc
     3361 acttccccgc cgtcgccgac gtagacattc tggcgcgagc ctggcccaac aatggacagc
     3421 ggcgtcccac cgacatcaac ggtgcgcatg tagctgaccg agtactgcat cgggttcgac
     3481 gccaacggcc ggcgcaggtc agccgtttcc ggcgaggccc cctcgctggc cgactccagg
     3541 tagactgtcg gcggaatctg gccgttgaac tcgaagacgc cgggcaggtt gtcgtgggcg
     3601 gccaggaagg agtgagccag gccggcggcc cgctcggccg agtcgggcaa ggcgcgcggc
     3661 cgttccggct cagtccacag ttcgctgagg tcctggtagt agaacgcgcc cgtgcgggcg
     3721 tcgatgcgca cctgcttggc gtcgccttcg ccgccgacca tgtagaaggc cgatccgtcg
     3781 ggcgcggcca gaatctccga gtcgaggccg aacgactggc cgatgttgcg gatgtagtcc
     3841 tcgttgacct cgcgcggccg gatcaggaag acgggcagct cctggaactc cgtggtgatc
     3901 tgcgccgggg gaggcgtgcc ggcgacgtga tcccagtacc agtagaggcc attgtaagag
     3961 ggatcggggc tgacatagcc ctggccccag atgtagtcgt tgtaattgtc aagttcctcg
     4021 gccagcaccc gggcgcgcac gccgctgggc tgctggtcgt cggtggccgt gaaccacgcc
     4081 tgggtgacgg tcttggctcc gtggccgaag atccaccaac ccttggcgcg catctggtcg
     4141 ccccacgcgc cgccgtcgcc ggggtaaacg acatacatgg tgttggcaaa gccgagcatc
     4201 aagtggaggc cgtcgaaggt ggtgtaccag taagcggcgc tgttgtcgtc gaggacgctg
     4261 cacgagtccc aggccagcca ttccaggtca tcgtcgccga aggcatggta ggcttcgccg
     4321 ggcgagaggt gcaagtcatc gtggttggtg ctgtaataga ccgaactgag gttcttgctc
     4381 cagaagctat cccacgtgcc agagccgtgg gtggccatca ggcccagatc gacggtgtcg
     4441 atccagatgt gctctgtgcc gccactggcc gcgcccttga agtcctcttc ccaggccagg
     4501 ctattgcccc agaagaagcg ctgcaaccag cccccatcta ccaactcggc gcgcaagcca
     4561 ttggccgagt ggtaccagtt gggacggtcg tcggccgtac cgggccagtc ggtgatccat
     4621 tcgatgccga actcagccgt gccaccgtcg tcgagggtgg cgtccgcgcc ggccggcgct
     4681 tgcggccgtt cctgctctgc tccggaaaca aaggccatgc tgagcatcag gctcagcagc
     4741 gccagacaga cagccagcag cctgcggata gaccagccat tgccgtgcat attggaacct
     4801 ccattgtctg caagtaggta atgccgctgc gaatggcacc ttcgcagagg cccgggccaa
     4861 ctcataagag cggcaacacg cgtgacgtag gggggcgcgg tggaggagag gatggcgcca
     4921 gtgggctgcc ggacggaccg ttgatcagca tagcataggg ccaatggact tgtcaatgac
     4981 cagcctgaag taatgttatg cttcagcgaa tctgacaccc cttaaggaaa aagtgaaaat
     5041 gtcacccaca tggacaaggt acattgaacg agaaggaaca gaaacgactg atggtactca
     5101 acgaggtgct ggctgggcgt cgaatagcgc acgaagcgca aagtagcaca gcctgttagg
     5161 ctgtcctcgc caaccgcggc cacagccaca gccacagcct gacaggctgt gctacacgtc
     5221 gaatagcgcg cgaagcgcaa agtagcacag cctgttaggc tgtcttcgcc aaccgcggcc
     5281 acggccacag ccacagccac agcctgacag gctgtgctac acgtcgaata gcgcgc
//
LOCUS       DAOSMQ010000200         5330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15533_length_5330_cov_5.058957, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000200 DAOSMQ010000000
VERSION     DAOSMQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15533_length_5330_cov_5.058957"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1522
                     /gene="cysS"
                     /locus_tag="PK829_07915"
                     /pseudo
     CDS             80..1522
                     /gene="cysS"
                     /locus_tag="PK829_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
     gene            1497..2285
                     /gene="rlmB"
                     /locus_tag="PK829_07920"
     CDS             1497..2285
                     /gene="rlmB"
                     /locus_tag="PK829_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOU41177.1"
                     /translation="MAPPGSSAEALVRRHPVLEALRAQRRAMQRLYVAGERGRAGREL
                     QPILAAAQAAGVPVTTLDKRALDQLAQRHAADAKHQGVLLETGPYPYAEPDEMLGLAE
                     RRGERPLLLLLDLLHGPQNIGALLRTAEACGVHGVILQDRRAPEITPSVVQFAAGATE
                     HLLIAKVTNLAETMRRLKDAGVWLAGMDLDDTAEELGRVDLDRALGIVVGHEGEGLRR
                     LVRDRCDFLVRLPMRGRIESLNAAVAGSILLYAAWQARGFNQER"
     gene            2291..2752
                     /locus_tag="PK829_07925"
     CDS             2291..2752
                     /locus_tag="PK829_07925"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU41178.1"
                     /translation="MNHSSAFYLPAGHDRFGEHRGLGISSIQFKVTPSDSGGILILEN
                     TFHARGGPARHLHHDQDEWFYAAEGEFVVEVGGERFALRPGDALLAPRRVPHVWAFTG
                     EGRGRMLITFLPAGQMEAFFREVTRADAMPMQDPALWRAYGMELIGPPLAI"
     gene            complement(2741..3115)
                     /locus_tag="PK829_07930"
     CDS             complement(2741..3115)
                     /locus_tag="PK829_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41179.1"
                     /translation="MNVQRSDDYREGLFLLIAGAAFAAMGLGQFDDWHGIQPWYFYTL
                     GVGLFIVTIIIAPRTKWTHSQQRRMWLPLIIAVVVSAILAIVLDEVRFVSIFPLVLGL
                     LAMGTGLWLMLRPKRTAGGSDR"
     gene            3611..4678
                     /gene="pheS"
                     /locus_tag="PK829_07935"
     CDS             3611..4678
                     /gene="pheS"
                     /locus_tag="PK829_07935"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOU41180.1"
                     /translation="MGLPNLRINFPESSMLEDLEQHYRQSAAALVAAEGREALAAWHR
                     DTLGKKGSIYRLTRNMAALSAEERPAFGRRLNEIKAQLEAGYDQRLAEAEAAALDAQM
                     AAGALDVTLPGRRPAHGRLHITTRTLRDIYRIFGDMGFQVYRSPDVETDEMNFELLNF
                     PPHHPAREMQDSFYTTTPGVILRTHTSAGQIRAMREYYPDPIRVVLPGMCYRNEQVTA
                     RSEMQFTQVELLAVGDDISFADLKGTLTDFARRLFGADRQTRFRASYFPFTEPSAEMD
                     ISCILCNGAGCSICKYTGWLEILGCGMVHPVVLRNGGYDPERYFGFAAGMGPERIAML
                     KYGIDDIRQFWANDLRFLEQF"
     gene            4784..>5330
                     /locus_tag="PK829_07940"
     CDS             4784..>5330
                     /locus_tag="PK829_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41181.1"
                     /translation="MRVPLSWLREYVDIDLSVAEVAEILTNAGLEVESIEYLGLPGAE
                     LEWDRERVVLAQVLRVEQHPDADRLVLATVDYGAAAPKVAVTGAPNLFAYIGRGDLSE
                     LRLFAPYALEGATLYDGHKEGRVKARLKGRPLRGIYNDSMLCSEKELGISEEHEGIIL
                     VRSDEIRAGEQGSRGAGERERA"
BASE COUNT          987 a         1816 c         1701 g          826 t
ORIGIN      
        1 aggttccagg gcccagggaa gagcgctttg ccctaatccc tggaacctgg cccctatcca
       61 catcaggagt cacagaacta tgtcgctcaa aatctacaac gttctcaccc gacaaaaaga
      121 ggcgtttgtg cccgtgacgc cgggcaaggt caacatctac gtctgcggcc cgacggtcta
      181 cgactactcc cacatcggcc acgccaagac ctacgtcagc ttcgatgtcg tcgtgcgcta
      241 tctgcgcttc tctggcaacg atgtcctcta cgtgcagaac atcaccgacg tcggccacat
      301 gctcgacacg ggcgaggacc gcatcctgaa gaaggctcgc caggcctcgg ccctgcccat
      361 gcaggtcgtc gagacgtaag cccgcgccta cttcaaggac atggacgcgc tgggggtgcg
      421 ccggccagac atcagcccgc gcgccagcgg ccacattccc gaacagatcg ccatgatcga
      481 gaagctgctg gccgatgggc acgcctacgt cgtcgatggc tcggtctact tcagcgtggc
      541 ctcgttcccg gagtacggca agctaagcgg gcgcgtcatc gaagacctgg aggagggcac
      601 gcgcgtcgtc gtccgccagg agaagcgcca cccggccgac ttcgccctgt ggaagcgggc
      661 cgagccggag cacatcctgc gctggactag cccctggggc gagggcttcc ccggctggca
      721 catcgaatgc tcggccatgg ccgccaagta cctgggcgag acgttcgaca tccacggcgg
      781 cggcatcgac aacatcttcc cccataacga gtgcgagatc gcccagagcg aggccgccca
      841 cgacgcgccc ttcgcccgct actggatgct aaccggctcg ctgacgctgg agggtgtcaa
      901 gatgagcaag agcctgggca acacgctgac gatccaggac gccctggcgc gctggcggcc
      961 agaggccatc cgcgccttca tcctgtccag ccactacagc agcccggtcg acttctccga
     1021 cgaggccatc gaggcggcct ataaaggctg gcagcgcatc tggggcgcgg tcaacctgac
     1081 acgcgaccag atgcgccgcg ccgcgccgtt cgaggccagc cctgaggtga ccgacatgct
     1141 gacggccgct cgcgcccagt tcatcgagcg catggacgac gacttcaaca cgccggcggc
     1201 tctggccgtg ctgcaagacg tgacccggca ggtgaacgcg ctgctcaacg agggcggccc
     1261 gcagacgctg gccacgctgg agaccatcga cgccttctac cgcgacctgg gcggcgaggt
     1321 gctgggcatt gtgccggagc gcgtcgagag cgccaccgac gccgcccgcg aggatggcct
     1381 ggtgcggctg ctgatcgacc tgcgcgccga ggcgcgggcc aaaaaggact gggccacggg
     1441 cgacaccatt cgcaaccggc taaaggaact gggcgtcgtc ctggaggatc gccccgatgg
     1501 caccacctgg aagctcggct gaggccctcg tccgccgcca cccggtgctg gaggcgctgc
     1561 gcgcccaacg gcgagccatg cagcgcctct acgtggccgg cgagcgcggc cgggccggcc
     1621 gcgagcttca gcccatcctg gccgcggccc aggccgccgg cgtgccggtg acaacgctcg
     1681 acaagcgcgc cctcgaccag ctggcccagc gccacgccgc cgacgccaag caccagggcg
     1741 ttttgctgga gaccggcccc tacccctacg ccgagccgga cgagatgctg gggctggccg
     1801 agcggcgcgg cgagcggccg ttgctgctgc tgctcgacct gctgcacggg ccgcagaaca
     1861 tcggcgcgct gctgcgcacg gccgaggcgt gcggcgtcca cggcgtcatc ctgcaagacc
     1921 gccgcgcccc ggagatcacg ccctcggtcg tccagttcgc cgccggggcc accgagcacc
     1981 tgctcatcgc caaggtgacc aacctggccg agacgatgcg gcggctgaag gatgctggcg
     2041 tgtggctggc gggcatggac ctggacgaca cggccgagga gttgggccgc gtcgatctcg
     2101 accgggcgct gggcattgtc gtcggccacg agggcgaagg gctgcgccgg ctggtgcgcg
     2161 accgctgcga cttcctggtg cggctgccca tgcgcggccg gatagagtcc ctcaacgccg
     2221 ccgtggccgg gtcgatcctg ctctatgccg cctggcaggc gcgcggcttc aatcaggaga
     2281 gataaacgcg atgaaccatt cgtcggcgtt ctacctgccc gccggccacg accgctttgg
     2341 cgagcaccgc ggcctgggca tcagttcgat ccagttcaag gtcacgccgt cggacagtgg
     2401 cggcatcctc attctggaga acaccttcca cgcccgcggc gggccggccc gccatctgca
     2461 ccacgaccaa gacgagtggt tctatgccgc cgagggcgag ttcgtcgtcg aagtgggcgg
     2521 cgagcgcttc gcgttgcggc cgggcgacgc gctgctggct ccgcggcgcg tgccccacgt
     2581 ctgggccttc accggtgagg gccgcggccg gatgctgatc accttcctgc ccgccggcca
     2641 gatggaagcc ttcttccgcg aagtgaccag ggctgacgcc atgcccatgc aagacccggc
     2701 cctgtggcgg gcctacggca tggaactcat tggaccgcca ctagcgatct gagccgcccg
     2761 ccgtccgctt tggccgcaac atgagccaca ggccagtgcc catggcgagc agccccagga
     2821 cgagcgggaa gatcgagaca aatctcacct cgtccaagac gatggccagg attgcactga
     2881 caaccacggc gatgataaga ggcagccaca tgcgtcgctg ctggctgtgc gtccattttg
     2941 ttcgcggcgc gatgatgatc gtgacaatga acagcccgac ccccaacgta tagaaatacc
     3001 acggctggat gccatgccag tcatcgaatt gccccaggcc catcgccgcg aatgccgcgc
     3061 cggcgatcag caagaaaagc ccttcccggt aatcgtcact gcgctgaacg ttcatcacat
     3121 cctctaccaa ttcccgcgcc agacgcgcca gctagttaca tcttaaaacc accggcgcga
     3181 cgtgtctata gagggcatac acgccagtgt aagcaaatcg tagcggagcg cgcatcgact
     3241 attgagtacg tgtctaccgc ttggccagta gcgccgccgc agagcttttc ttgacaagcc
     3301 ctgcccacaa tgttactatc tccgccatga gtagtgactg ttcacgctcg ttttttagct
     3361 ttgcttttgc gaacatgtcc cgcgccgcga cggccgagac gctctgttcc gcctgagcaa
     3421 ggcacgcgac agagaagacc gaaagacgcg gcgcaggccg cgtctttttt gttggcaaag
     3481 gaagaacggt taacaggttg acagacaagc tcacgcagag gcgcaaagaa agcaaagacg
     3541 ccaaggaaga aacacggggc ttcttttctt ccttagcgcc tttgcccctt agcgcctttg
     3601 cgtgagcctc atgggtctac ccaatctgcg gataaacttt ccggagagtt ccatgctcga
     3661 agatctagaa caacactacc ggcagtccgc cgcggcgctg gtggcggccg aggggcgcga
     3721 ggcgctggcc gcctggcacc gcgacacgct gggcaagaag ggcagcatct accggctgac
     3781 gcgcaacatg gccgcgctca gcgccgagga acggcctgcc ttcggccggc gtctcaacga
     3841 gatcaaggcc cagctggagg ccggctacga ccagcgcctg gccgaggccg aagccgcggc
     3901 gctggacgcg cagatggccg ccggggcgct ggacgtgacc ctgcccggcc gccgcccggc
     3961 ccacggccgc ctgcacatca ccacgcgcac cctgcgcgac atctaccgca tcttcggcga
     4021 catgggcttc caggtctatc gcagccccga cgtcgagacg gacgagatga acttcgagct
     4081 gctcaacttc ccgccccacc acccggcgcg agagatgcag gacagcttct atacgaccac
     4141 accgggcgtc atcctgcgca cccacaccag cgccggccag atccgcgcca tgcgcgagta
     4201 ctaccccgac cccatccgcg tcgtcctgcc gggtatgtgc taccgcaacg agcaagtcac
     4261 tgcccgcagc gagatgcagt tcacccaggt cgaactgttg gccgtcggcg acgacatctc
     4321 cttcgccgac ctgaagggca cgctgaccga cttcgcccgc cgcctgttcg gagccgaccg
     4381 acagacgcgc ttccgcgcca gctatttccc cttcacggag ccgagcgcgg agatggacat
     4441 cagttgcatc ctgtgcaacg gcgcgggctg ctccatctgc aagtacaccg gctggctgga
     4501 gattctgggc tgcggcatgg ttcatcccgt cgtgctgcgc aacggcggct atgacccgga
     4561 acggtatttc ggtttcgccg ccggcatggg gccggagcga atcgccatgc tgaagtatgg
     4621 catcgacgac atccgccagt tctgggccaa cgatttgcgc ttccttgaac aattctaaag
     4681 gaaagccagg gggctgaggg gcaggggagc gggggagaaa gagatcaccc ccgctcccct
     4741 gcccccggct cccctgcacg ctggccattt ttctggagac aacatgcgcg taccgctatc
     4801 gtggctgaga gaatacgttg acatcgacct gtccgtggcc gaagtcgcgg aaatattgac
     4861 caacgccggc ctggaggtcg agagcatcga gtacctcggt ctgcccggcg cggaactgga
     4921 gtgggaccgt gagcgcgtcg tcctggccca ggtgttgcgc gtcgagcaac accccgacgc
     4981 cgaccggctg gtgctggcca cggtcgatta cggcgcggcc gcgccgaagg tggccgtcac
     5041 tggcgcgccg aacctgttcg cctacatcgg ccgcggcgac ctgagcgagc tgcgcctctt
     5101 tgccccctac gcgctggagg gggccacgct ctacgacggc cacaaagagg gccgcgtcaa
     5161 ggccaggctg aagggccggc cgttgcgcgg catctacaat gactccatgc tctgctccga
     5221 gaaggaactg ggcatcagcg aggagcatga gggcatcatc ctggtgcgga gtgatgaaat
     5281 aagagcaggg gagcagggga gcagaggagc aggggaaaga gaaagagccg
//
LOCUS       DAOSMQ010000201         5325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15547_length_5325_cov_4.313472, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000201 DAOSMQ010000000
VERSION     DAOSMQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15547_length_5325_cov_4.313472"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /gene="mazG"
                     /locus_tag="PK829_07945"
     CDS             <1..1266
                     /gene="mazG"
                     /locus_tag="PK829_07945"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU41182.1"
                     /translation="RLGRAGEIVYAVPGHPFVGEATVAAITRGAAEAGIPVEIVAGLS
                     FVEPVLAALGIDALDGLQLFDAIELASYLYPPINPDVPLLLGQVYSRLLAGQLKAALM
                     SIYPPEQPVALIHAAGEAEQRVEHLALHEFDRSQHIDHLTSLYVPALPVKAGLTALAE
                     TVAVLRSPDGCPWDQEQTSQSLREGFLEEAYEALAAIDAEDESNLREELGDLLYHIVM
                     QTQIAAEADSFTLTEVIAGIEAKLKRRHPHVWGDWQVSSSADVLRNWEILKQREKSDA
                     GATPASVLDGIPPALPALARSQKIQGKAANTGFDWPNIEGVFDKLAEEVAEVRAATTA
                     GEKRAELGDVLFVVVNLARWLGADAETALREANERFMRRFRGVERLAAGRGLALSSLS
                     FEALDGLWQEVKAALAQSDVADTITSEPE"
     gene            1286..1918
                     /locus_tag="PK829_07950"
     CDS             1286..1918
                     /locus_tag="PK829_07950"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU41183.1"
                     /translation="MTTVLNIVLLSLAALAALGALTFLFRGLGARSKINRQAYSVGQV
                     EARRTTALNWIRAGFLFVAALVFMGIFLARPLLSGRAADPTPAPTLPVATTAPQASPA
                     ATTPLEPAPVPAGQASPTVLPASPTPEATAAPTATTAVQTATVSSGVGVWLRGAPSTT
                     GEQLEWLLDGTAVTLLPGQQTADDLLWQQVRTAAGVEGWVARDFLTVEGQ"
     gene            complement(1959..3758)
                     /locus_tag="PK829_07955"
     CDS             complement(1959..3758)
                     /locus_tag="PK829_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41184.1"
                     /translation="MAPLMRGIEAEKYDRAYSDRDLVARILGYFKPYRKKIIVIVVAI
                     AIMAAFGALVPIVIANSLNTEASEAPFPTTLAALVFVAGVGTWALAGIRRYLTAQVVA
                     DVVNAMRNDAFAASMRQDLSFFDKYSTGRIVSRITSDTQEFGEVLAISTDVINQLATA
                     IILIFVLFSIDLKLSLAVLVMTPLVVAVALLFRRMARDVTRQSSRALAEVNKAIQEAV
                     SGIRVAKNFRQEQAIYDEFMQVNNQAYSINVRRALVLANIFPTLNALAGIGTALLVYF
                     GGRSAIAGAISISSWYIFVATVDSFWFPVTNLSAFWSQFQAGLAATERVFALMDTETS
                     VRQTASEPAGRLNGDIDFEHVSFRYSSQEQVLDDFSLRIQPGESVALVGHTGAGKSSI
                     IKLIARYYEFQEGTIRVDGHDIRDFDLTAYRRQLGIVGQVPFLFAGTVADNIRYACPD
                     ATDERIEATARQIGRGEWLETLPEGIASEVGERGARLSMGQRQLVALTRVLLQNPSIF
                     ILDEATASIDPFTESQIQEALDLIMARRSAIIIAHRLSTVRSADRIIVLRAGKIIEEG
                     SHQSLMAQSGHYAELYDTYFRHQSPSYAVRNGG"
     gene            3840..4850
                     /gene="trmB"
                     /locus_tag="PK829_07960"
     CDS             3840..4850
                     /gene="trmB"
                     /locus_tag="PK829_07960"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOU41185.1"
                     /translation="MSPAIWSKIEQLPWPADWPALFGRDAPLVMEIGFGSGQYLVALA
                     ARRPEANILGVEISVPALRRAGRKLERRDLSHVRLIQGDASAVLWALCAPGSLAAVVI
                     NFPDPWPKQGHDARRLIDDGFLCLLATRVAADAPLDIATDHDGYAAQVAAALERSPHF
                     ESRSGAPFALEQPGRVPTKYERVALAEGRTPRYFAWRRNDAPATTPFSIPEEYAMPHV
                     VLRGPADLAEIGRRFRPTVVMFDGGRVRFIEAYRALGEDKLLIETYINENPIRQRLGL
                     EIRPRANGEIVLGLAEVGFPRPTRGVHAAVAALSGWLRAEFPALIVVHAALQEEYAHA
                     ED"
     gene            4831..>5325
                     /locus_tag="PK829_07965"
     CDS             4831..>5325
                     /locus_tag="PK829_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU41186.1"
                     /translation="MPTLRTNGIDLSYETRGQGEAVLFLHGLGSRSEDWQLQVPAFAA
                     NYEVVTADMRGHGRSSKPPGPTSVPMMADDVLGLLDTLGLASAHVVGLSMGGMIAFQL
                     AADHPERVRSLVIVNSGPELVAHSLADRLRIWQRLLLARLFNPERTASFLGPRLFPKP
                     EQAQL"
BASE COUNT          849 a         1726 c         1849 g          901 t
ORIGIN      
        1 cgcctggggc gcgcgggcga gatcgtctac gccgtgcccg gccacccctt tgtgggcgag
       61 gcgacagtgg cagccatcac gcgtggcgcc gccgaggcgg gcatccccgt ggagatcgtc
      121 gccgggctga gcttcgtcga gccggtgctg gccgcgttgg gcatcgacgc cctcgacggc
      181 ttgcagctct ttgatgctat cgagttggcc agctatctct acccgcccat caacccggac
      241 gtgcccttgt tgctgggaca ggtctatagc cgcttgctgg ctgggcagct aaaggcggcg
      301 ctgatgagca tctacccgcc ggagcagccc gtggccctca tccacgctgc tggcgaggcg
      361 gagcagcgcg tcgaacacct ggcgctgcac gagtttgacc gcagccagca cattgaccac
      421 ctgactagcc tctacgtccc cgcgctgccg gttaaggcag ggctgaccgc cctggccgag
      481 acagtggcgg tgttgcgcag ccccgacggc tgtccgtggg accaggagca gacctcgcaa
      541 agcctgcgcg agggctttct ggaggaggct tatgaggccc tggcggccat tgacgccgag
      601 gatgagtcca atctgcgcga ggagctgggc gacctgctct accacatcgt catgcagacg
      661 cagatcgccg ccgaggccga cagcttcacc ctgaccgagg tcatcgccgg catcgaggcc
      721 aagctgaagc gccgccaccc gcacgtctgg ggcgactggc aggtcagcag ctcggccgac
      781 gtgctgcgca actgggaaat cctgaagcag cgcgagaaga gcgatgccgg cgcgacgccc
      841 gcctcggtgc tggacggcat tccgccggct ctaccggctc tggcccgctc gcagaagatt
      901 cagggcaagg cggccaacac tggcttcgac tggcccaaca tcgagggcgt cttcgacaag
      961 ctggccgagg aagtggccga agtgcgcgcc gcgacaacgg ccggcgagaa gcgggccgaa
     1021 ctgggcgatg tgctgtttgt ggtcgtcaac ctggcccgct ggctgggcgc ggacgccgag
     1081 acggccctgc gtgaggccaa cgagcgcttc atgcggcgct tccgcggcgt cgagcggctg
     1141 gcagccgggc gcgggctggc cctgtcgtct ctgtcgttcg aggcgctgga tggcctgtgg
     1201 caggaggtca aagcggcgct tgcgcaatcg gacgttgctg atacgattac gagcgaaccg
     1261 gagtaggtcc ggtgcgtcat agaccgtgac aacagtactc aacatcgtcc tcttgtcgct
     1321 ggcggccctg gcggccctgg gagccttgac cttcctattc agagggttgg gcgctcggtc
     1381 gaagatcaac cgccaggcct atagcgtggg gcaggtggaa gccagacgga cgaccgcgct
     1441 gaactggatt cgagcgggct ttctgttcgt cgctgccctt gtctttatgg gcatcttcct
     1501 ggcccggccg ctgctgagtg gccgcgccgc cgacccgacc cctgcgccga cgctacctgt
     1561 ggccacgacc gcgccccagg catcgcccgc cgccaccacc ccgctcgagc ccgcccccgt
     1621 gcccgctggc caggccagtc cgaccgtgct gcccgcctcg cccacgcccg aagccacggc
     1681 cgcgcctacg gccacgaccg ccgtgcagac ggccacggtc agcagcggcg tcggcgtctg
     1741 gctgcggggc gcgcccagca cgaccggcga gcagctggaa tggctgcttg acggcactgc
     1801 ggtcaccctg ctgcccggcc agcagacagc cgatgacctg ctctggcagc aggtgcggac
     1861 ggccgccggc gtcgaaggct gggtggcgcg ggatttcctg actgttgaag ggcagtagta
     1921 gcgcgtgtcc agccgccggt gatcagtggt cggccgggtt agcccccgtt cctgaccgcg
     1981 tagctaggcg actggtggcg gaagtaggtg tcgtatagct cggcatagtg gccgctctgg
     2041 gccatgagcg actggtggct gccctcttcg atgatcttcc ccgcgcgcag aacaatgatg
     2101 cggtcggccg agcgcacggt cgagagccgg tgggcgatga tgatggccga gcgccgggcc
     2161 atgatcaggt cgagcgcttc ctgaatctgg gactccgtga agggatcgat gctggccgtg
     2221 gcctcgtcga ggatgaagat gctggggttc tgcaacagca cgcgcgtcag ggcgacgagc
     2281 tggcgctggc ccatcgacag gcgcgcccct cgttcgccga cctcgctggc aatcccctcc
     2341 ggcagcgttt ccagccactc gccgcggccg atctgccggg cagtggcttc gatgcgctca
     2401 tcggtggcgt cggggcaggc ataacggatg ttgtcggcca ccgtgccagc gaagaggaac
     2461 ggcacctggc caacgatgcc caactgccgc cggtaggccg tcaggtcgaa gtcgcggatg
     2521 tcgtgaccgt cgacccggat cgtaccctcc tggaactcgt agtagcgagc gatgagcttg
     2581 atgatgctcg acttgcccgc gccggtgtgg ccgacgaggg cgacgctctc gcccggctgg
     2641 atgcgcaggc tgaagtcgtc gagaacctgc tcctgtgatg agtagcggaa ggagacgtgc
     2701 tcgaagtcga tgtcgccgtt gaggcggccg gccggttcac tcgccgtctg gcggacgctg
     2761 gtttcggtgt ccatcagggc gaagacgcgc tcggtggccg ccaacccggc ctggaactgg
     2821 ctccagaagg ccgacaggtt tgtcaccgga aaccagaaac tatcgaccgt ggccacgaag
     2881 atataccagg aggagatgct aatcgccccg gcaatggcgc tacgtccgcc gaagtagacc
     2941 agcagagccg tgcctatgcc cgccagggcg ttgagcgtgg ggaagatgtt ggccagtacc
     3001 agagcgcggc ggacgttaat gctataggcc tggttgttga cctgcatgaa ctcgtcgtag
     3061 atggcctgct cctgccggaa gttcttggcc acgcggatgc cgctgactgc ttcttgaata
     3121 gccttgttga cctcggccaa tgcgcgcgaa ctctggcggg tcacatcgcg ggccatgcgc
     3181 cggaacaaca gagccacggc aacgacgagc ggcgtcatga ccagcaccgc cagcgacagc
     3241 ttcagatcga tgctaaagag gacgaaaatg aggatgattg ccgtggccaa ctggttaatc
     3301 acgtcggtac tgatggccag cacctcgccg aactcctgtg tgtcgctagt gatacggctg
     3361 acgatgcggc cggtggagta tttgtcgaag aacgacaagt cctggcgcat ggacgcggcg
     3421 aaggcgtcgt tgcgcatggc gttgacaacg tcggcgacga cctgggcggt cagatagcgg
     3481 cggatgccgg ccagcgccca cgtgccgacg ccagcgacaa ataccagcgc ggccagcgta
     3541 gtcggaaagg gtgcttcgct ggcctcagtg ttgaggctat tggcgatgac gatgggcacc
     3601 aacgcgccga acgcagccat gatggcgatg gccacgacga tgacgatgat cttcttgcgg
     3661 tagggcttga agtagccgag gatgcgggca acgaggtcgc ggtcgctgta ggcgcggtcg
     3721 tacttttctg cttcgatacc acgcatgagt ggggccataa ggagggtatt gtggcccaag
     3781 ggggccgatg gcgcaaggct ccgtttgccg gcgccggggg cagacgtata atcgccgcga
     3841 tgtcgccggc gatctggtcc aaaatcgagc aactgccgtg gccggccgat tggccggcgc
     3901 tgttcggccg cgacgcgccg ctggtcatgg agatcggctt cggcagtggc cagtaccttg
     3961 tcgcgttggc cgcgcgccgg ccagaggcca acattctcgg cgtggaaatc tccgtgcccg
     4021 cgctgcggcg ggcggggcgc aagctggagc ggcgcgacct gagccacgtc cgcctgattc
     4081 agggcgacgc cagcgcggtg ctgtgggcgt tgtgcgcgcc gggcagcctg gccgcggtgg
     4141 tcatcaactt ccccgacccc tggcccaagc agggccacga cgctcgccgc ctcatcgacg
     4201 acggcttcct ctgcctgctg gctacgcggg tggccgcgga cgcgccgctg gacatcgcca
     4261 ccgaccacga tggctacgcc gcgcaggtcg ctgccgctct ggagcgctcg ccccacttcg
     4321 agagccgcag cggggcgccc tttgccctgg agcagcccgg ccgcgtgccg accaagtatg
     4381 agcgcgtcgc cctggccgag gggcgcacgc cgcgctactt cgcctggcgg cgcaatgacg
     4441 cgccggcgac cacgcccttt tctatcccgg aggagtatgc catgccgcat gttgtcttgc
     4501 gcgggccggc cgacctggcg gagatcggcc gccgcttccg ccctacggtg gtgatgttcg
     4561 acggcgggcg cgtgcgtttc attgaggcct accgcgctct gggcgaggac aagctgctca
     4621 tcgagaccta catcaacgag aaccccatcc gccaacggct ggggctggag atacggccac
     4681 gcgccaacgg tgagattgtc cttgggctgg ccgaagtcgg ctttccgcgg ccgacgcgcg
     4741 gcgtgcatgc cgcggtggcc gcgctgtccg gctggctacg ggcggagttc cccgcgctca
     4801 ttgtcgttca tgcggcgtta caggaggagt atgcccacgc tgaggactaa tggcatcgat
     4861 ctgtcctacg agaccagggg gcaaggggag gcggtgctgt ttctccacgg tctcggttcg
     4921 cgtagtgagg attggcagtt gcaagtgcct gccttcgcgg ccaattacga ggtggtgacg
     4981 gccgacatgc gtggccacgg ccgctcgtct aagccgcccg gccccaccag cgtgcccatg
     5041 atggccgacg acgtgctggg cctgctcgac acgctgggcc tcgcttcggc ccacgtcgtc
     5101 ggcctgtcga tgggcggtat gatcgctttc caactggccg ccgaccaccc ggagcgcgtg
     5161 cgcagcctgg tgatcgtcaa cagcgggccg gagttggtag cccattcgct ggccgaccgg
     5221 ctgcgcattt ggcagcgtct gctgctggcc cgccttttca acccggagcg aacggccagt
     5281 ttcctcggcc cgcgcctctt ccccaagccg gagcaggcgc agttg
//
LOCUS       DAOSMQ010000202         5320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15566_length_5320_cov_4.227160, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000202 DAOSMQ010000000
VERSION     DAOSMQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15566_length_5320_cov_4.227160"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK829_07970"
     CDS             <1..308
                     /locus_tag="PK829_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41187.1"
                     /translation="HTQTFTIPIDPPTGQADITVQVALVDNDKDARAITVSAAAGAAS
                     DSDVSTGPTNGPTLSLITLNLNDVAGETSEVVITLQTDEQGDSAAVVGAAVHYACEP"
     gene            508..1032
                     /locus_tag="PK829_07975"
     CDS             508..1032
                     /locus_tag="PK829_07975"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOU41188.1"
                     /translation="MSTPTDEERRANRARLGQTLTAIAALWAVVAALTIVGAWGEMQR
                     ARAAAGWATVSGAVRAVEVVPVEVRGRAPVRRPGVHVEYAYTVAGQSFTGRRVQPVER
                     RIDPQTALGKSLLALAPGDSIPVYVNPNDPAQAVLRRDVSWRGVVNGFLLLVLAAAVG
                     VVARGIPAGPGRVG"
     gene            complement(1039..1701)
                     /locus_tag="PK829_07980"
     CDS             complement(1039..1701)
                     /locus_tag="PK829_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41189.1"
                     /translation="MAKQSRPDFRPARGTFMKGILVILMVFGIIILAAVALALASNFG
                     NTATPTLAPPPQGNLVATPTPAAAALPTVPPAGQPTALPPVGDGATDGATSPFNGSFA
                     GTLSTGNGSSAPVNLALTQTGTIVSGDINIGDGLTIDGGNCGAQAIPAGGRAVSGQTD
                     PSNPNHIEVSAGYDVQGITITARLTADLSADGNAITGRIDLDLPFFCGSDPVITGALA
                     RQ"
     gene            1821..2606
                     /locus_tag="PK829_07985"
     CDS             1821..2606
                     /locus_tag="PK829_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41190.1"
                     /translation="MRTLKTILAVLAIVLAVITILTAGVGIYYAWALNTPVTESLTAM
                     STTVEGVLTRADAGLTRVDAAIDTARAATSTIEGAVMAAGDRITSTDIAFTVLDRTVG
                     DRLFPPLTEAAATITAVVDTVVAANDALEAANRLPFVNVPTITTELQVAADQVNAARE
                     QVNQIRAEVQTMKESAVSRPVTAVTDRTGALLGHLDTAKSTTTTTLATIARTQDRLVL
                     ARERLPRTLDLLSVGLTLLLLWFIIGQAALINVSWRWLRAARI"
     gene            complement(2706..4577)
                     /locus_tag="PK829_07990"
     CDS             complement(2706..4577)
                     /locus_tag="PK829_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOU41191.1"
                     /translation="MLTHLRHLLLGSPIPSADTGELRLDKVRALAALSPDALASVAYA
                     NQEIYLGLLVAGAAGLNHAFSLALVISGLLALLTLSYRQTIAAYPGGGGSYTVARENL
                     GDTPGLTAAAALLIDYVLNAAVSVTAGVAALASAFPALWPYRTALALLLLLVITLVNL
                     RGVREAGTVMTIPVYLFLATYLGMIVWGIGVVLVRGPGAFPPALTAPAGAGGASAAAL
                     VPVTIFLLLRTFASGATALTGVEAISNAVPIFHEPVVRNAQRTMAAMAALMAVLFLGT
                     VGLTQALAIVAGPEETILSALARHLFGSGPVYLLVQVATLLVLIVAANTSYTGFPRVV
                     SLMARDGFLPRQLRSVGDRLVFSNGILLLAVLAALLVVVFGGDSHALIPLFAVGAFLA
                     FTLSQAGMVVHWTRARGPGWGWRAALNGLGATATGVALLVIGSSKFRGGAWIVILLLP
                     VLVWLFRSVRRHYNEVGRQLHLTNDAPPVGCTRHPRVVMPVAGVHRGVVEALNFACSI
                     ADDVIAVYVELEPDSGPAMHARWEAAGMARVARLITVPSPYRSLLGPFLDCIDQLDAE
                     RPDDWPVTVLIPEFVPARWWHFLLHNQTALPLKWALIYRRHRDGKARAVIDVPFYLD"
     gene            4822..>5320
                     /locus_tag="PK829_07995"
     CDS             4822..>5320
                     /locus_tag="PK829_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41192.1"
                     /translation="MKPTRLPVLCLLVLLALAACGQGAAEVAPTAPPPTATAAPTATP
                     IPTATPIPTATPAPTATPRPTPTPQPTEVACDLDRLAEAVDNQTRLTSYRQQATAYAQ
                     FAGQQLRQRLLTISGSVEMAGGEVSATDMRLVSYTDAGRAMHIIMVADQFYFSEAGGP
                     WQSALP"
BASE COUNT          885 a         1747 c         1926 g          762 t
ORIGIN      
        1 cgcacacgca gaccttcacc atccccatcg acccgcccac gggccaggcc gacatcaccg
       61 tgcaggtagc gctggtggac aacgacaagg acgcgcgggc gatcaccgtc tcggccgcgg
      121 ccggcgcggc gagcgacagc gacgtgtcca ccggcccgac caacggcccg acgctgagtc
      181 tcatcaccct gaacctgaat gatgtggccg gggagacgag cgaagtggtg attacgctgc
      241 aaacggacga gcagggcgat tcggccgctg tggtgggcgc ggcggtccat tacgcctgcg
      301 aaccataacc gcgccgcaac cgtaggggca ggcctgcgtg tctgcccctc ttgctcggct
      361 agggaggggg tttgattcgg ccgtcggagg tcacagcgcc ctctccctaa ccctctcccg
      421 gggggagagg gaaccagagt ccggagggag agggaaccag agttgggaaa gttagggagg
      481 ggagtgggtt tgggttacac tgggcgcatg agcacgccaa cggacgaaga gcggcgagcc
      541 aaccgggcgc ggctggggca gacgctgacg gccatcgccg cgctgtgggc tgtggtggcc
      601 gcgctgacta tcgtgggggc gtggggtgag atgcaacggg cgcgggccgc ggccggctgg
      661 gccacggtca gcggcgcggt gcgcgctgtg gaggtcgtcc cggtggaggt gcgcgggcgc
      721 gcgccggtgc ggcggccggg cgtgcatgtg gaatacgcct acaccgtcgc cgggcagtcg
      781 ttcaccggcc gccgcgtgca accagtggag cgccgcattg acccgcagac cgccctgggt
      841 aagtcgctgc tggccctcgc ccccggcgat agcatccccg tttatgtcaa cccgaacgat
      901 ccggcgcagg ctgtgctgcg gcgcgacgtg tcgtggcggg gcgtggtcaa tggctttttg
      961 ctgctggtgc tggccgcagc cgttggcgtg gtcgcgcggg gcataccggc cggcccaggc
     1021 cgcgttgggt agtcgcgcct attgccgcgc cagcgcgccg gtgatgacgg ggtcgctgcc
     1081 gcagaagaag ggcaggtcga ggtcgatccg gccggtgatg gcgttgccgt cggcggagag
     1141 gtcggccgtt agcctggcgg tgatggtgat gccctgcacg tcgtagccgg cgctgacttc
     1201 aatgtggttg gggttcgacg ggtcggtctg gccgctgacg gcgcgcccgc cggcggggat
     1261 ggcctgcgcg ccgcagttgc cgccatcaat ggtcaggccg tcgccgatgt tgatgtcgcc
     1321 gctgacaatc gtgccggtct gtgtcagggc caggttgacc ggcgcgctgc tgccgttgcc
     1381 ggtgctgagc gtaccggcga aggagccgtt gaatggggag gtcgcgccgt cggtcgcgcc
     1441 gtcgccaacg ggcggtagcg ccgtgggctg gccggcgggc ggcacggtgg ggagggcagc
     1501 cgcggccggg gtgggcgtgg cgacgaggtt gccctgtggt ggcggcgcaa gcgtcggcgt
     1561 ggccgtgttg ccgaagttcg acgccagcgc cagagccacg gcggccagga tgataatgcc
     1621 gaagaccatc agaatgacca gaattccctt catgaacgtc cctcgcgccg gccgaaagtc
     1681 cggccggctc tgtttggcca tatgataccc aatggcccca cggccaggcg cggcgctagg
     1741 caaatcctca agaaatcaac gcccctaccc ggccggatag aatgggataa aatgtctcat
     1801 ctccccggag ggaaacaacg atgcgcactc tgaagaccat tctcgccgtt ctggccattg
     1861 tcctggccgt catcaccatt ctcaccgccg gcgtgggcat ctactacgcc tgggcgctga
     1921 atacccctgt gaccgagtcg ttgacggcga tgtccaccac cgtcgagggc gtgctgaccc
     1981 gtgccgatgc cggcctgacc cgcgtcgacg cggccatcga cacggcccgc gcggccacca
     2041 gcaccattga gggcgcggtc atggccgccg gcgaccgcat cacgtccacc gacatcgcct
     2101 ttaccgtcct ggatcgcacc gttggcgacc ggctctttcc gcccctgacc gaggcggcgg
     2161 cgacgataac ggccgtggtc gatacggtcg tggccgccaa cgacgccctg gaggcggcca
     2221 accggctgcc gttcgtcaat gtgccgacga tcaccacgga gctgcaagtc gccgccgacc
     2281 aggtgaacgc cgcccgcgaa caggtcaacc agattcgcgc cgaggtgcag acgatgaagg
     2341 aatcggccgt ctctcgcccg gtgacggccg tcaccgaccg gacgggggcg ctgctgggcc
     2401 acctcgatac ggccaagagc acaacgacca cgaccctggc cacgattgcc cggacgcaag
     2461 atcgactggt gctggcccgc gaacggctgc cccgaacgtt ggacttgctg tcggtgggtc
     2521 tgacgctgct ccttctgtgg ttcatcatcg gacaggcagc gctcatcaac gtctcgtggc
     2581 ggtggttgcg cgcagcgcgg atctagcgcg gcggttgtgg tggcgaccaa ctggcccgag
     2641 cgggagctga caggtgcgcg gccgggggcc gtctaccggt caggccggcg cgtcagccct
     2701 gctctctaat caagatagaa cggcacatcg atcaccgccc gcgccttgcc gtcgcggtgg
     2761 cggcggtaga tcaaggccca cttcagcggc agggcggtct ggttgtgcag cagaaagtgc
     2821 caccagcgcg cgggcacgaa ctcggggatg agcaccgtca ccggccaatc gtcggggcgc
     2881 tcggcgtcga gctggtcgat gcaatcgagg aaggggccga gcagcgagcg gtagggcgag
     2941 ggcacggtga tgagccgggc gacgcgggcc atgccggccg cctcccagcg ggcgtgcatg
     3001 gccgggccgc tgtcgggttc taactcgacg tagacggcga tgacgtcatc ggcaatggag
     3061 caggcgaagt tgagcgcctc gaccacgccg cggtggacgc cggccacggg catgacgaca
     3121 cgtgggtggc gggtgcagcc caccggtggg gcgtcgttgg taaggtgcag ctggcggccg
     3181 acctcgttgt agtggcggcg caccgagcgg aacagccaca ccagtacggg cagcagcagg
     3241 atgacgatcc acgccccacc gcggaatttg ctgctgccga tgaccagcag ggccacgccc
     3301 gtggccgtcg cccccagccc gttgagcgcc gcccgccagc cccagcccgg cccgcgggcg
     3361 cgcgtccagt ggacgaccat gcccgcctgc gacagcgtga aggccaggaa cgcgccgacg
     3421 gcgaacagtg ggatgagggc atgggagtcg ccgccgaaga cgaccaccag tagcgccgcc
     3481 agcaccgcca gcagcaggat gccgttggag aacaccagcc ggtcgccgac gctgcgcagt
     3541 tgccgcggca gaaagccgtc gcgggccatc agcgagacga cgcgtgggaa gccggtgtag
     3601 ctggtgttgg cggcgacgat cagcaccagc agcgtggcca cctgcaccag cagatagacc
     3661 ggcccggaac cgaacagatg ccgcgccagg gcggagagga tcgtctcttc cggcccggcg
     3721 acgatggcca gggcttgcgt caggccgact gtgcccagaa agagcacggc catcagggcg
     3781 gccatggccg ccatcgtccg ctgggcattg cgcacgaccg gctcgtggaa aatgggcacg
     3841 gcgttgctga tcgcctcgac gccggtgagg gccgtcgcgc cgctggcgaa ggtgcgcagc
     3901 agcaggaaga tcgtcaccgg gaccagcgcc gcggcggatg cgccgcccgc gccggccggc
     3961 gccgtcagcg cgggcgggaa cgcgcccggc ccgcgcacca gcaccacgcc gatgccccag
     4021 acgatcatgc ccagataggt ggccaaaaag aggtagacgg gaatagtcat gaccgtgccc
     4081 gcctcgcgca cgccgcgcag gttgacgagg gtgatgacca gcagcagcag cagcgccagc
     4141 gccgtgcgat atggccacag ggcggggaag gccgacgcca gcgcggccac gccggcggtg
     4201 acgctgacgg cggcgttgag gacgtagtcg atcagcagcg ccgccgcggc ggtcaggccg
     4261 ggggtgtcgc ccaggttctc gcgggcgacc gtgtagctgc cgccgccgcc ggggtaggcg
     4321 gcgatggtct ggcggtagga cagtgtcagc agggccagca ggccgctgat gaccagggcc
     4381 aggctgaagg cgtggttgag gccggccgcc ccggccacca gcaggcccag atagatctcc
     4441 tgattggcgt aggcgaccga ggccagcgcg tcgggcgaca gggccgccag ggcgcgcacc
     4501 ttgtcgaggc gcagctcgcc cgtgtcggcc gaggggatgg gggagccgag gaggaggtgg
     4561 cgaaggtggg ttagcatcac aactcgctat gattgccaaa gtcaaaccag agcaggataa
     4621 tccttgtaac gcgatttgcc aaatcgcgca atgtggcgcg attttccaaa tcgcgatgtg
     4681 gtgcgatttt ccaaatcgcc atgtggtgcg attttccaaa tcgcgatgtg gcgcgatttt
     4741 ccaaatcgcc atgtggcgcg attttccaaa tcgcgtcaca gagggcgatt tgccaaatcg
     4801 ccctacaaga cgggaggagc catgaagccc acacgattgc ccgtcctttg tctgcttgtc
     4861 ctgttggccc tggcggcgtg cggccagggc gcggccgagg tcgcgccgac cgccccgccg
     4921 cctacggcca cggccgcccc cacggccaca cccatcccca cggccacgcc catccccacg
     4981 gccacgcccg cgcccacggc caccccccgg ccgacgccga cgccccaacc gaccgaagtc
     5041 gcctgcgacc tggaccggct ggccgaggcg gtggacaacc agacccgcct gaccagctat
     5101 cggcagcagg ccactgccta cgcccagttt gccggccagc agttgcggca acggctgttg
     5161 acgattagcg ggtctgtcga aatggccggc ggcgaggtga gcgccacgga catgcgcctg
     5221 gtgtcctaca ccgatgccgg tcgggcgatg cacatcatta tggtggccga ccagttctac
     5281 tttagcgagg caggcgggcc gtggcagtca gcgctcccgg
//
LOCUS       DAOSMQ010000203         5287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15698_length_5287_cov_4.923547, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000203 DAOSMQ010000000
VERSION     DAOSMQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15698_length_5287_cov_4.923547"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="ccsA"
                     /locus_tag="PK829_08000"
     CDS             complement(<1..893)
                     /gene="ccsA"
                     /locus_tag="PK829_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOU41193.1"
                     /translation="MNPATIVNPRLDRAIRIMTILAVVGMVITIGAIFLFAPVELNMG
                     NVQRLFYFHVGSAWVGAMVFGVALVCGLLYLWRGRRVFDTVSVASVEVGLVFLSMAIV
                     GGSFWGKPAWNTWWLWSPRLTLVTVAWLVYAAYFMLRGAIEDDHRRARYAAIYAIVSF
                     VTIILTYVSIRIFRDIHPVVFGGTTEAAAGASEGLQDFNGLDSARMGITLLISVIGFT
                     LLGSAWILMRIRLQNLIDYADSLKTRVATRLSARPRPAIVSGLALVLFQVGEPNQFNA
                     YLIGGYMVMSSLCLLYMLYLA"
     gene            complement(970..1671)
                     /locus_tag="PK829_08005"
     CDS             complement(970..1671)
                     /locus_tag="PK829_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOU41194.1"
                     /translation="MSAFLATVWAVVWKDLRIEGRTRQTISIMVMFSVATIIMFNFGL
                     GMAININLDAGRNVSPALLWAVVLLAAILGLNRSLALDREDQVFDAQLIAPISRNALY
                     AGKVISISLFTLLLDLILVVLFTVFFNRPFYLPLVLLVLTLGTIGYVAAGVLITSMTI
                     QARTREVLLPVLLLPISLPLVLPAAMATTTYINAAMLGEASWAAVRAPVLLVAAYDLL
                     MLAVGFLTYRFVVES"
     gene            complement(1668..2366)
                     /gene="ccmA"
                     /locus_tag="PK829_08010"
     CDS             complement(1668..2366)
                     /gene="ccmA"
                     /locus_tag="PK829_08010"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HOU41195.1"
                     /translation="MIRLNAVVKQYGLNPVLRGVDLHVQAGEFVTLVGPNGAGKSTLM
                     AIVATLLRPTSGEVRVGGWPLPEAADRVRQHIGLVSHHALLYRDLTAAENLAFTARLY
                     RLPDAEPRIMDALRTVGLFARQRDPVGTFSRGMLQRLTIARATLHEPDVLLLDEPYTG
                     LDQEASALLDELLREQSARGRTILMITHDLGHGLGLADRLAILYRGQIAREVARADIS
                     PAAVYDLYAEVTLA"
     assembly_gap    2412..2511
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2512..3393)
                     /locus_tag="PK829_08015"
     CDS             complement(<2512..3393)
                     /locus_tag="PK829_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU41196.1"
                     /translation="MDDHTEQTNLPTDNLARALWHIYRRPEATLWQGGGNLPWNDPAF
                     STRMLAEHLDESHGAASRQAAERAAQIDWLWAQLRLEPGRRLLDLTCGPGLYAVALAR
                     RGVHVTGVDFSPAAIAYARQLAGYKRVSDRCAFVEADVRAYEPEPGSYDAVLILYGQL
                     AVFPREEAAALLSKAARALRPGGRLVVELLDQERVDKTDSTWWFADDKGLWGDRPFLN
                     LGERRWDANARASIERYTTIELETGGLSEIVLCDQTYGVGEMIALMQAAGFSSVSAHA
                     RWAGLPLYDAEEWVVYVA"
     gene            3486..>5287
                     /locus_tag="PK829_08020"
     CDS             3486..>5287
                     /locus_tag="PK829_08020"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HOU41197.1"
                     /translation="MNEQERQNRAVMLERVKTVWLQGVLEESLHGGEIIDLGFAYRPA
                     ALGNSGVPSWQQAAEYDYLLPLGTTIAGVFSAAERVLLVLGEPGAGKTTMLLQLVSRL
                     LAEAEADASCPMPAVFALSAYTADRPLADWLVDQLANNYETPRRLAAQWIDAGQFVPL
                     LDGLDEVEATLRPACAAAINAFRQQHPGVAMLVTARSRDYQALATRLNMDKAIVLQPL
                     TMAQIDAYLARRGPRLAGLRDSLAADRTLRELAQTPLMLSIMTLAYRRMPEGGEGMGA
                     ALLTRTQLFEVYAERMARYRSKDMRFSPSDTIAWLSWLARQLIREGKPTFFLEDMQPA
                     WLDAGDRPRFGRDMRLLVFMMLSGASLPAVFIALIFGRWGAAAAVLLVGLLAAAVPAL
                     TGRFLLRARLPFDRIETVESLRWSWPYAALGLGIGAAGGLALGALLGWIGRGGVAPWA
                     ALLAAAGGTLGAVEAALRPGDLRLRMTPGQGISQSFQSGRTVLRRISVLPVILFSATL
                     FVAMSLSGRLIVLAIAPWLMWLMAYLNLGFALAYGWLAYFQHLWLRRRLYDRGHIARD
                     YPAFLNQAAERNLLRKVGGGYTYVHALLLQYFGER"
BASE COUNT          874 a         1751 c         1744 g          818 t
ORIGIN      
        1 atggccagat agagcatgta cagcaggcac agagacgaca tgaccatgta gccgccgatc
       61 aggtaggcgt tgaactgatt cggttcgccg acctggaaga ggacgagggc caggccggag
      121 acgatggccg ggcgtggccg ggcgctcagc cgggtggcca cgcgcgtctt caggctgtcg
      181 gcatagtcga tcaggttttg caggcggatg cgcatcaaaa tccaggctga ccccagcaga
      241 gtgaagccga tgacgctgat gagcagggta atgcccatgc gcgccgagtc aaggccgttg
      301 aagtcctgta acccctcgga cgcgccggcg gcggcttcgg ttgtgccgcc gaagacaacc
      361 gggtggatgt cgcggaagat gcggatgctg acgtaggtca ggatgatggt gacgaaggag
      421 acgatggcgt agatggcggc gtagcgggcg cggcggtggt cgtcctcaat ggccccgcgc
      481 agcatgaagt aggccgcgta gaccagccag gcgaccgtca ccagcgtcag ccgtgggctc
      541 cacagccacc acgtgttcca ggccggcttg ccccagaacg agccgccgac gatggccatc
      601 gacaggaaga cgaggccgac ctcgaccgac gccacggaca cggtgtcgaa cacgcgccgg
      661 ccgcgccaca ggtacagcag gccacatacc agggccacgc caaagaccat cgctcccacc
      721 caggccgagc cgacgtggaa gtagaacagc cgttgaacat tgcccatgtt gagttccacc
      781 ggcgcgaaga ggaagatcgc cccgatggtg atgaccatgc ccacgacggc caggatggtc
      841 atgatgcgaa tggcccggtc aagtcgcgga tttacaatcg tcgctggatt catgatgact
      901 tactccagat aagacagaat cgaccacgaa cgacggttcc cccttgcgtc gctcgtggct
      961 ggtcgccggc taggactcta ccacaaaacg gtacgtcagg aaacccacgg ccagcatgag
     1021 caggtcataa gccgcgacca gcagcaccgg cgcgcgcacg gccgcccacg acgcctcgcc
     1081 cagcatggcc gcgttgatat aggtcgtcgt ggccatggcc gcgggcagca ccagcggcag
     1141 cgatatcggc agcagcagca ccggcagcag cacctcgcgc gtgcgcgcct gaatggtcat
     1201 ggaggtgatc agcacccccg cggccacgta gccgattgtg cccagcgtca gcaccagcag
     1261 caccagcggc agatagaacg gccggttgaa gaacactgtg aacagcacca ccaggatcag
     1321 gtcgagtagc aatgtgaaca ggctgatgct gatgactttg cccgcgtaga gagcattgcg
     1381 cgagatgggg gcgatgagtt gggcgtcgaa gacctgatcc tcgcggtcga gggccagcga
     1441 gcggtttagc cccaggatgg ccgccagcaa caccaccgcc cacagcagcg ccggcgagac
     1501 gttgcgcccg gcgtcaaggt tgatgttgat ggccatgccc agcccaaagt tgaacatgat
     1561 gatcgttgcc actgagaaca tgaccatgat gctgatcgtc tggcgcgtgc gcccctcgat
     1621 gcgcaggtct ttccagacca cagcccacac agtcgccagg aacgcgctca tgccagtgtc
     1681 acttcggcgt agaggtcata gaccgccgcc gggctgatgt cggcgcgcgc gacctcgcgg
     1741 gcgatctggc cgcgatagag aatggccagc cggtcggcca gccccagccc gtgccccagg
     1801 tcgtgggtga tcatcaggat cgtccggccg cgggccgact gctcgcgcaa cagctcatcc
     1861 agcagggcgc tggcttcctg gtcgaggccg gtgtatggct cgtcgagcag caacacgtcc
     1921 ggctcgtgga gcgtcgcccg ggcgatggtt agacgttgca acatgccgcg cgaaaacgtc
     1981 ccgacggggt cgcgctggcg ggcaaagagg ccgaccgtgc gcagggcatc catgatgcgc
     2041 ggctcggcgt cgggcaggcg atagaggcgg gcggtgaagg ccagattttc ggccgcggtc
     2101 aggtcgcgat agagcagggc gtggtgggaa acaaggccaa tgtgttggcg cacgcggtcg
     2161 gcggcctccg gcagcggcca gccgccgacg cgcacctcgc cggaggtcgg ccgcagcagc
     2221 gtggcgacga tggccataag cgtgctcttg cccgccccgt tcggccccac cagggtgacg
     2281 aactcgcccg cctgcacgtg cagatctacg ccgcgcagca ccggattgag gccatattgt
     2341 ttgacgacgg cgttgaggcg gatcattgag gtgaattgta ggggattggg gcgttgcggc
     2401 aaaccacaac gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggccacgta
     2521 caccacccac tcctccgcat catacaacgg cagccccgcc cagcgggcat gggccgatac
     2581 gctgctaaaa ccggccgcct gcatcagggc gatcatctcg ccaacgccat acgtctggtc
     2641 gcagaggacg atctcgctca gcccgcccgt ctccagctca atcgtcgtgt agcgctcgat
     2701 cgacgcccgc gcgttggcat cccagcgccg ttcgcccagg ttcaggaagg gcctgtcgcc
     2761 ccatagcccc ttgtcgtcgg caaaccacca cgtgctgtcg gtcttgtcga cgcgctcctg
     2821 gtcgagcagc tccaccacca gccgcccgcc cggccgcagc gcccgcgccg ccttgctcag
     2881 cagcgccgcg gcctcctcgc gcggaaagac ggccagctgc ccgtagagga tcagcacggc
     2941 gtcatagctc cccggctctg gttcataggc gcgcacgtcg gcctcgacga aggcgcagcg
     3001 gtcgctgacg cgcttatacc cggccagttg ccgggcgtag gcgatggccg ccgggctgaa
     3061 gtcgacgccg gtcacgtgga cgccgcgccg cgccagggcg acggcgtaga ggcccggccc
     3121 gcaggtcagg tccagcagcc gccggcccgg ctccaggcgc aactgcgccc acaaccagtc
     3181 gatctgcgcc gcccgctcgg ccgcctgcct tgaggccgcg ccgtggctct cgtccaggtg
     3241 ctcggccagc atccgcgtcg aaaaggccgg gtcattccag ggcagattgc cgccgccctg
     3301 ccacaacgtc gcctccggcc gccggtagat gtgccacagg gcgcgggcca ggttgtcggt
     3361 gggaaggttg gtttgttcgg tgtggtcatc catcggccac gattatagct cgatttcacc
     3421 cctctttttc atgcgcagcc ggccaatgtg ggtactctat tggtagcgac tttccgcgga
     3481 gggacatgaa cgaacaggag cggcagaacc gggcagtcat gctggagcgg gtgaaaaccg
     3541 tctggttaca gggcgtgctg gaggaatcgc tgcacggtgg ggagatcatc gacctcggct
     3601 ttgcctaccg gccggcggcg ctgggcaaca gcggcgtgcc cagctggcaa caggcggccg
     3661 aatacgacta cctcctgccc ctgggcacga ccatcgccgg cgtcttcagc gccgccgagc
     3721 gcgtcctgct ggtgctgggc gaaccgggcg cgggcaagac gacgatgctg ctgcaactgg
     3781 tcagccgcct gctggccgag gccgaggccg acgcgagttg ccccatgccg gccgtcttcg
     3841 ccctgtcggc ctacaccgcc gaccgcccgt tggccgactg gcttgtcgac caactggcca
     3901 acaactacga gacgccgcgc cggctggccg cgcagtggat cgacgccggc cagttcgtcc
     3961 ccttgctcga cgggctagac gaggtcgagg cgacgctgcg gccggcctgc gccgcggcca
     4021 tcaatgcctt tcgccagcag catcccggcg tggccatgct cgtcaccgcc cgcagccgcg
     4081 actaccaggc cctggccacg cgcctgaaca tggacaaggc catcgtcctg caaccgctga
     4141 cgatggcgca aatcgacgcc tatctggccc ggcgcgggcc gcggctggcc ggcctgcgcg
     4201 actcgctggc cgccgaccgc accctgcgcg aactggccca gacgccgctg atgctcagca
     4261 tcatgaccct ggcctaccgg cgcatgcccg aaggcggcga gggcatgggc gcggctctgc
     4321 tgacgcgaac gcaactcttt gaggtctacg ccgagcgcat ggcccgctat cgtagcaagg
     4381 acatgcgttt ctcccccagc gacaccatcg cctggctgag ttggctggcc cggcaactca
     4441 tccgcgaggg caagccgacg ttcttcctgg aggatatgca gccggcgtgg ctggacgccg
     4501 gcgaccggcc gcgcttcggg cgagacatgc gcctgctcgt cttcatgatg ctatcggggg
     4561 cctccctgcc ggcggtcttc atcgccctca tcttcggccg ctggggcgcg gcggcggcgg
     4621 tgctgctggt cgggctgctg gccgcggccg tgcccgccct gaccggccgc tttctgctgc
     4681 gcgctcgact gccgttcgac cgcatcgaga cggtcgagtc gctgcgctgg tcgtggccct
     4741 atgccgcgct ggggctgggc atcggcgcgg ccggcgggct ggccctgggg gcgctgctcg
     4801 gctggatcgg ccgcgggggc gtcgccccct gggcggcgct gctggccgcc gccggcggca
     4861 cgctgggcgc ggtcgaggcc gcgctgcggc cgggcgactt gcggttgcgg atgacgccgg
     4921 ggcagggcat ctcgcagtcg ttccagagcg gtcgcaccgt cctcagacgg atcagtgttc
     4981 tgccggtgat cctgtttagc gcgaccctct ttgtagccat gtcgctcagc ggccggttga
     5041 ttgtcctggc catagcgccg tggctgatgt ggctgatggc ttatctcaac ctgggtttcg
     5101 ccctggccta tggctggttg gcctacttcc aacacctgtg gctccgccgg cggctctacg
     5161 accgcggtca cattgcccgc gactaccccg ccttcctcaa ccaggccgcc gagcgcaatc
     5221 tcctgcgcaa ggtcggcggc ggctatacct acgtccacgc gttgctgcta caatactttg
     5281 gcgaacg
//
LOCUS       DAOSMQ010000204         5281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15727_length_5281_cov_4.635285, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000204 DAOSMQ010000000
VERSION     DAOSMQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15727_length_5281_cov_4.635285"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2805)
                     /locus_tag="PK829_08025"
     CDS             complement(<1..2805)
                     /locus_tag="PK829_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41198.1"
                     /translation="MEPESIFLTLLRKEDFLMLTVQAVNMHLEGDLLVRTNAAEPAVL
                     LYHFPPQHTAEEAFHQNKAGDEPAGPVPVMAWLAGASRLAFRLPEGVETLPVTLEALL
                     DWRALEPVLPPNALPPDATTGPAPAAPAFNQTALELPTGLLLSPDASAGWLHDTTPHE
                     RDGRFALWQTRLGRRDVDAEGNPITAASDDRAVRVVWSTRPAIAFTNSLTNTDRHEAV
                     TLSSDFTLPRPPGWAIVHPVRLMIWRQRLAASGLPLRYIPRPARARRLMLSSLGAWTN
                     LESEWDYPIIIPDSPQADLGYPTLTLEQWTHQMAQGRDQYARTVRTAFVCDCAHRISI
                     VTVTERQFQPTLVRTDQTPDGRVAIFGSVAYLRQYEYFIIQKQLQDYEGQAGLFQYAG
                     RELPYRRLRITMRVSPIIDPRPADDAPFWPTVGGQPLLFPMIGEDWDGQPSTTQKTQL
                     VVPLRAMIARRSTIDGQPDPKVWTWPEIVALYNAGDAGRRTADLRGQSVAFAANIDTE
                     PGKTRLDTQSITTQAQLVTAPTPLGVPPFLPKMETARVTIPAVNRVLGREESVDIRYD
                     ATYLSDGFSPGSNPGEVFVKLVKPLAVAVAADRSGGMVRPESGVEGLSRNAGAVAKAD
                     KFKTAELDLSAFDSFRLLGTMTIRDILPKVPIDMAALREAQGAPDTVLQRLHDPDFFV
                     QAPVLVGSLAYPPGADPRTTPPAAAITRLVWKPRIQAFEFGPFFAFGLNTNGQLYLTA
                     TLTTPLDGGSPTFVIDGALEQFALHFLSSLRINFVALRFRAVDGCKPDISAEGVDLVF
                     EGPLTFVNTLRDILPADGFSDPPYVTVDGAGILAGYTLAVPSLGIGIFSLQNLSLAAQ
                     LSVPFVGKPASVRFAVSERHKPFLVSVTLFGGGGFFAMALSADGLEQIEAAIEFGGNL
                     SLNLGVASGGVYVMAGIYFSL"
     gene            3023..4312
                     /locus_tag="PK829_08030"
     CDS             3023..4312
                     /locus_tag="PK829_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOU41199.1"
                     /translation="MKIAVIGGGSTYTPELVNGFLARAETLPLTELWLMDIDAERLAV
                     VGGFARRMVAARGAPFRVVLSDDRRASIAGAAYVVTQLRVGMMPARRGDEYLGRRHGL
                     IGQETTGVGGMAKALRTIPVLLDIAADMRATAPGALLANFTNPAGLVTEALTRYAPDV
                     PTVGVCNVGITAKMRLLAAVEALTGQTIAPERAQLNTLGLNHLTWHRGFTVDGEEMWP
                     QIMAAAVAQARREPQPEWDAATLATLGMIPNYYLQYFYYTAHKLAEQEAWPPSRAEQV
                     MAIEAELLRDYANPALVAPPPDLMKRGGAYYSTLATQVIDSHANDRGQIHVVNTRNDS
                     AVAEWPADWVLELPARIDRAGVHPLPAAPLPPPVFGLVAQVKMYELLTVEAAVHGDRR
                     AAHAALLAHPLGPAADQVAAVLDDMLATNQAWLPQFR"
     gene            4319..5224
                     /locus_tag="PK829_08035"
     CDS             4319..5224
                     /locus_tag="PK829_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOU41200.1"
                     /translation="MSPNPLLQHLGFGPDDRVVIIHVDDVGMCHASLQAFIDLWDAGT
                     ISSGAVMVPCPWFPATAAWARANPGVDLGVHATLNAEWSGYRWGPVSTRNRATGLLDD
                     DGYFHARETGAWANADPAAAQAEVEAQVDAALAAGIDVTHIDTHMGTITHLRFIQGYL
                     QTLFRYRRPGLLPRLTSAQFLAYGITGDPAAADLFAGMVAGLEAAGMPLIDAVDYLPL
                     DDPTDHIGLARRKLAALPPGITHFLMHPAIDTPELRAIAPDWPSRVANYQAFISREMR
                     DFLRASDIHLIGYRPLREYMAAATI"
BASE COUNT          874 a         1803 c         1780 g          824 t
ORIGIN      
        1 caggctgaag tagataccgg ccatgacgta gacgccgccg ctggccacgc cgaggttaag
       61 cgacagattg ccgccgaact cgatggccgc ctcgatctgc tccaggccgt cggcgctgag
      121 ggccatggca aaaaagccgc cgccgccaaa cagggtcacg ctgacgagaa acggcttgtg
      181 gcgctcggag acggcgaagc gcaccgaggc gggcttgccc acgaagggca ccgagagttg
      241 agcggccaga gataggtttt gcaggctgaa gatgccaatg cccagcgagg gcacggccag
      301 ggtgtagccg gccaggatac ccgcgccatc gacggtgacg tagggcggat cgctgaagcc
      361 gtcggcgggc aggatgtcgc gcagagtgtt gacgaaggtg agtgggcctt cgaaaaccag
      421 gtcaacgcct tcggccgaga tatcgggctt gcagccgtcc acggcacgga agcgcagggc
      481 gacgaaattg atccgcagcg aactgagaaa gtggagcgcg aactgctcca acgcgccatc
      541 aatgacgaac gtcggcgacc caccgtcgag cggcgtggtc agggtcgccg tcagatagag
      601 ttggccgttg gtgttcaggc caaaggcgaa gaaggggcca aactcgaacg cctgaatcct
      661 cggcttccaa accaggcggg taatggccgc ggccggtggt gttgtacgtg ggtcggctcc
      721 cggcgggtag gccagagacc cgaccagaac cggggcctgc acgaagaagt cggggtcgtg
      781 cagccgctgc aggacagtgt cgggcgcgcc ctgggcctcg cgcagcgcgg ccatgtcgat
      841 gggcactttc ggcaagatgt cgcggatcgt catcgtgccc agcagccgga agctgtcgaa
      901 ggctgacagg tctagctcgg ccgtcttgaa cttgtcggcc ttggccaccg cgccggcatt
      961 gcgtgacaag ccctcgacgc cactttccgg ccgcaccatg ccgccagacc tgtcggccgc
     1021 cacagccaca gccaacggct taacgagctt gacaaagacc tcgccagggt tactgcccgg
     1081 gctgaagccg tcgctcaagt aggtggcatc atagcggata tccacgctct cctcgcggcc
     1141 gaggacgcgg ttgaccgccg ggatggtcac ccgcgccgtc tccatctttg gcaggaaggg
     1201 cggcacgccc agcggcgtcg gggcggtcac gagttgggcc tgggtggtaa tgctctgcgt
     1261 gtcgaggcgc gtcttgcccg gttcggtgtc gatgttggcg gcaaaggcga cgctctggcc
     1321 gcgcaggtcg gccgtgcgcc ggcccgcgtc accggcgttg tagagcgcca cgatctccgg
     1381 ccacgtccat accttcggat cgggctggcc gtcgatggtc gagcgccggg cgatcatggc
     1441 ccgcagcggc acgacgagct gcgttttctg ggtcgtggac ggctgcccat cccagtcctc
     1501 gccgatcatc gggaacaaca gcggttgacc gccgacggtg ggccagaagg gcgcgtcgtc
     1561 ggctgggcgc gggtcgatga tgggcgagac gcgcatggtg atgcgcaggc ggcgataggg
     1621 caactcgcgg ccggcatact ggaacagccc tgcctgcccc tcatagtcct gaagttgctt
     1681 ctggatgatg aagtactcgt actgtcgcag ataagcgacc gagccgaaga tagccacccg
     1741 cccgtcgggc gtctggtcgg tgcggacgag ggtcggctgg aattgccgct cggtgacggt
     1801 gacgatggag atgcggtggg cacagtcgca gacgaaagcc gttcgcaccg ttcgcgcgta
     1861 ctggtcacgc ccctgggcca tctggtgcgt ccattgttcc agcgtcaggg tggggtagcc
     1921 caggtcagcc tgtggcgagt cggggatgat gatcgggtag tcccactcgc tctccaggtt
     1981 cgtccacgcg cccagcgacg agagcattag gcgccgggcg cgggcgggcc gggggatgta
     2041 gcgcagcggc aggccgctgg cggccagcct ctgccgccag atcatcaggc gtaccgggtg
     2101 gacgatagcc cagccgggcg gccgcggcag ggtgaagtca ctcgacagcg tgaccgcttc
     2161 gtggcggtcg gtgttggtca gcgaattggt gaacgcgatg gccggccgcg tgctccagac
     2221 gacgcgcacc gcgcggtcgt cgctggcggc ggtgatggga ttgccctcgg cgtccacgtc
     2281 gcggcggccg aggcgcgtct gccagagggc gaagcggccg tcgcgctcgt ggggcgttgt
     2341 gtcgtggagc cagccggcgc tggcatcggg cgagaggagc aggccggtcg gcagttccag
     2401 cgccgtctgg ttgaaggccg gggcggccgg cgcggggccg gtggtcgcgt cgggcggcag
     2461 ggcattcggc ggcagcactg gctccagcgc ccgccaatcg agcagcgcct ccagtgtcac
     2521 cggcagcgtc tcgactcctt ccggcaggcg gaaggccaaa cgactggccc cggccagcca
     2581 ggccattacg ggaaccggtc cggccggttc atcgccagcc ttgttctggt ggaaagcttc
     2641 ttcggccgtg tgctgtggcg gaaagtgata gaggaggacg gccggttcgg ccgcgttcgt
     2701 gcgcacaagc aggtcgccct ctaggtgcat gttgaccgcc tgaacggtca gcatcaggaa
     2761 gtcctctttg cgcagcaggg tcaggaagat cgattctggt tccatatatc tcccaatcga
     2821 ccgctgtaga catgaaacga gccgccttgg tcaggccagg ttgcattgta ggagtgtcct
     2881 cagagagaag tcaatacgtc atatgaccct aagcgccgat aaggacgctc ggcgctgtgc
     2941 ttgggcaatc ttcccattgg cgctacactc ttcctggcgc gccgcaaaga tggccgccct
     3001 cttagaccac tggagcatcc ccatgaaaat cgccgtcatc ggcggcggct cgacttacac
     3061 gccggagttg gtcaacggct tcctggcgcg ggcggagacg ctgccgctaa ccgaactgtg
     3121 gctcatggac atcgacgcgg agcggctggc cgtcgtcggc ggcttcgccc ggcgcatggt
     3181 cgccgcccgg ggcgcgccct tccgcgttgt gctcagcgac gaccggcgag ccagcatcgc
     3241 cggcgcggcc tacgtcgtca cccagttgcg cgtcggcatg atgcccgccc ggcgtggcga
     3301 cgagtacctt ggccgccgcc acggcctgat cggccaggag accaccggcg tcggcggcat
     3361 ggccaaagcc ctgcgcacca tcccggtgct gctcgacatc gccgccgaca tgcgcgccac
     3421 tgcccctggc gcgctgctgg ccaacttcac caacccggcc gggttggtga ccgaggcgct
     3481 aacccgctac gcgcccgatg tgcccaccgt tggcgtctgt aatgtcggca tcacggccaa
     3541 gatgcgcctg ctggcggccg tggaggcgct gaccggccag accatcgccc cggagcgggc
     3601 gcagctcaac acactgggcc tcaaccacct gacctggcac cgcggcttca ccgtcgatgg
     3661 cgaagagatg tggccgcaga tcatggccgc ggccgtggcc caggcgcgcc gcgagccgca
     3721 gccggaatgg gacgcggcca cgctggccac gttggggatg atccccaatt actacctgca
     3781 atacttctac tacaccgccc acaagctggc cgagcaagaa gcctggccgc cgtcgcgcgc
     3841 cgagcaggtc atggctatcg aggcggagtt actgcgagac tatgcgaatc ccgccctggt
     3901 cgcgcccccg cccgacctca tgaagcgcgg cggcgcctac tactccaccc tggccaccca
     3961 ggtcatcgac agccacgcca acgaccgcgg ccaaatccac gtcgtcaaca cgcgcaacga
     4021 cagcgccgtg gccgagtggc cggccgactg ggtgctggaa ctgcccgccc gcatcgaccg
     4081 cgccggcgtc cacccgctgc cggccgcgcc cctgccgccg ccggtctttg ggctggtggc
     4141 ccaggtgaaa atgtatgaac tgctgaccgt cgaagccgcc gtccacggcg accggcgcgc
     4201 cgcccacgcc gcgctgttgg cccaccccct cggcccggcc gccgaccagg tcgctgccgt
     4261 gctcgacgac atgctggcca ctaaccaggc ctggctacct caattcaggt gatgtcccat
     4321 gtctcccaac ccgctgctcc aacacctcgg cttcggcccc gacgaccgcg tcgtcatcat
     4381 ccacgtcgat gacgtcggca tgtgccacgc ctcgctgcaa gcgttcatcg acctctggga
     4441 cgcggggacg atctcctccg gcgcggtgat ggttccctgc ccgtggttcc cggccacggc
     4501 tgcctgggcg cgggccaacc ccggcgtgga cctcggcgtc cacgccacac tcaacgccga
     4561 gtggtctggc taccgctggg ggccggtgag cacacgcaac cgggccaccg gcctgctgga
     4621 tgacgatggc tatttccatg cccgcgagac gggcgcatgg gccaacgccg accccgccgc
     4681 cgcgcaggcc gaggtcgaag cccaggtgga cgcggctctg gccgccggca tcgacgtaac
     4741 ccacatcgac acccatatgg gcacgatcac ccacctgcgc ttcattcaag gctatctaca
     4801 aacgctgttc cgctatcgcc ggccgggcct gctcccgcgc ctgacaagcg cgcagttctt
     4861 ggcctatggc atcaccggcg acccggcggc ggcggacctg ttcgccggca tggtcgccgg
     4921 gcttgaggct gctggcatgc ctctgatcga cgccgtggac tatctgcccc tcgacgaccc
     4981 gaccgaccac attggcctgg ccaggcgcaa actggccgcc ctgccgccgg gcatcaccca
     5041 cttcctgatg cacccggcca tcgacacgcc cgaactgcgc gccatcgccc ccgactggcc
     5101 cagccgcgtg gccaactacc aggccttcat cagccgcgag atgcgcgact tcctgcgagc
     5161 atccgacatc cacctcatcg gctaccggcc gctgcgcgag tacatggccg cggccaccat
     5221 ctgaacggtc atgggctact acctgggcat cgacgtgggc gcgaccaaga cccacgccct
     5281 c
//
LOCUS       DAOSMQ010000205         5247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15860_length_5247_cov_3.782935, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000205 DAOSMQ010000000
VERSION     DAOSMQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15860_length_5247_cov_3.782935"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PK829_08040"
     CDS             <1..595
                     /locus_tag="PK829_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU41201.1"
                     /translation="ASVTFLNTLLSALAAYAIAKIPFPGRNALFSFMLTTMMIPGILF
                     LIPTYVLMYNIGWVGHFQSLIVPAAVTVFNIFLLRQFMASIPNEVIEAGRLDGASELT
                     IFLRVILPLARPALATVAILNFMGSWNDFFGPLLYLNTPDKWTVQLGLMQFQSSVPGE
                     NAQQIWAATTLVTLPLVLLFFFLQDQFMKAFANVTFK"
     gene            603..1202
                     /locus_tag="PK829_08045"
     CDS             603..1202
                     /locus_tag="PK829_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41202.1"
                     /translation="MTTNLPAAQGAPAPAVAATARHAPWWRLPLILLAVVAIFVGTYF
                     LAWWDSRRLTARYMADADASFEAGNYLDALAGYEVFDEAANEYVRRGGYGDVERIWHH
                     PRARPVPAMAAQAAARIDEIINTRLTIAEAEQFVQENIGRTNPYMGVVYLRLGELYEA
                     DGDRRSAEDIYSEIEDLFPGEPELIARANANLARLQAEE"
     gene            1326..2870
                     /locus_tag="PK829_08050"
     CDS             1326..2870
                     /locus_tag="PK829_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOU41203.1"
                     /translation="MFSTISRHRFLLLLLLFVLVVAIVPVQAQEEPTVSPDAPKKSAV
                     RKELRQIASDTWDSFVAMTYPNGLPADNISATGVRAGYSSPTNIASYIWSTLVARDLG
                     IITQSEARQRIGQTLTTLATMERHGDSGMFYNWYDPATGAKLTTWPPSGDPVYPFLST
                     VDNGWLAAALIMVTNAVPPLRDEAQAILDDMNFGFYYDPGAGLLRGGFWDAVPPGCSI
                     QGNYPGQGADVYYTCHHYGTLNTEPRIASYIGIAWGQVPPTHYFKMWRTFPDTCDWSW
                     PEMKPEGVTRNYLGVDVYEGHYTYRGMDIVPSWGGSMFEALMVELMVPEAEWGPNSWG
                     INHPLYVRAQIEHGLEEAQYGYWGFSPATNPSGGYREYGVDAIGMNTDGYASNNDNTY
                     VDYGFGDCRPGQPIPPASAYTNGVVTPHAVFLALEFAPGATLRNMRNLRRDFDVYSDW
                     GFYDSVNVDTGQVSPYYLALDQGMIMAALGNYLRNDRLEFYFTHGEVRQAIRPLLAME
                     RFTAGD"
     gene            2953..>5247
                     /locus_tag="PK829_08055"
     CDS             2953..>5247
                     /locus_tag="PK829_08055"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HOU41204.1"
                     /translation="MLLPNDYGHFSDDGREYIITRPDTPMPWINVLTNGDYGLTLSQT
                     GGGYSWRGHASLNRLTRWSQDLVCDDWGKYLYIRDADSGDFWSAAWQPAGQLLAGYRV
                     RHGFGYTTIEWQRDTLGGALTTFVPLGDPCELWLLRLENHGPRSRRLQLFTYLEWLLG
                     AAPDWHREFHRLFIESRYDAGHHALLANKVLWELPGLPGPHLNRSWPYVAFHSSSRPP
                     AGYDGDKAAFLGRYGRTAAPRAVREGRSGDTAGRWGDAIASLHVPLTMAPSETVEIVF
                     ALGAADDAPAALALSDKYRSPAAAHAALDEARRFWAGLVDGLAVTTPDPALDVMANGW
                     LVYQAVAGRLWGRSAYYQTGGAYGFRDQLQDSLVWLLLDRPADTLAQIRLHAAHQYQE
                     GIVLHWWHPLAETGLRSRYSDDLLWLPFVVWHTIQETGDLAFLDEEVPFYDGGAASLW
                     EHCQRAFDVALARRSPRGLPLILEGDWNDGLNATGPAGRGESVWMAHFLYYLLTCWAT
                     LPIDAATRARFTAEAAALRAATEQHGWDGAWYWRATTDEGLQLGAAARVEGRIFLNAQ
                     TWATLSGLAAPDRTQQALAAARQHLYTDYGALLLFPAYSVPDPDVGYLTRYAPGTREN
                     GGVYAHAACWAVLAERQAAGAGAAYDLWRRMCPPRRAAAPDVYAAEPYVMPGNVNGPL
                     APRPGQGGWTWYTGAAAWYLRALVEGVLGVSAGLDGLSVAGDLPAEWDGFRLRRRFRG
                     ATYDIDVHRAAAGALVAGCCCDAGG"
BASE COUNT          823 a         1792 c         1717 g          915 t
ORIGIN      
        1 cgcgtcggtc accttcctca acaccctgct cagcgccctg gccgcctacg ccatcgccaa
       61 gattcccttc cccggccgca acgcgctctt cagcttcatg ctgacgacga tgatgatccc
      121 cggcatcctg ttcctgattc ccacctacgt gctgatgtac aacatcggtt gggtgggtca
      181 cttccaatca ctcatcgtgc ccgcggccgt caccgtgttt aacatcttcc tgctgcgcca
      241 gtttatggcc agcatcccta acgaggtcat cgaggccggg cgactggacg gggccagcga
      301 gctgacgatt ttcctgcgcg tcatcttgcc cctggcccgg ccggccctgg cgacggtggc
      361 catcctcaat ttcatgggca gttggaatga cttcttcggc ccgctgctct acctgaatac
      421 gcccgacaag tggacggtgc agctggggct gatgcagttc caatcgtcgg tgccgggcga
      481 gaacgcgcag cagatctggg cggcgacgac gctggtgacg ttgccgctgg tgctgctctt
      541 cttcttcctg caagaccagt tcatgaaggc gtttgccaac gtgacgttca agtgaggctg
      601 ccatgacaac gaacttgcct gccgcccagg gcgcgcccgc gccggccgtg gcggcgacgg
      661 cgcgccacgc gccctggtgg cgtctgccac tgatcttgtt ggccgtggtc gccatctttg
      721 tcggcaccta tttcctggcc tggtgggact cgcgccggct gacggcgcgc tacatggccg
      781 acgccgacgc cagcttcgag gccggcaact acctggacgc gctggccggc tatgaggtgt
      841 tcgacgaggc ggccaacgag tacgtgcgtc gcggcggcta tggcgacgtg gaacgcatct
      901 ggcatcaccc gcgcgcccgg cccgtgccgg ccatggccgc ccaggcggcg gcgcgcatcg
      961 atgagatcat caacacgcgg ctgaccattg ccgaggccga gcagtttgtc caggagaaca
     1021 tcggccgcac caacccgtac atgggcgttg tctatctgcg cctgggtgaa ctgtacgaag
     1081 ccgacggcga ccggcgttcg gccgaggaca tctacagcga aatcgaggat ctgttccccg
     1141 gcgaaccgga gttgatcgcg cgggccaacg ccaacctggc ccgcttgcag gccgaggagt
     1201 gacgcggccc tcttcagggg cgatggcatc accggcggat cacggaacgg cgactgccga
     1261 gtgtgcgact gctcggtgtt cgaccgctga gcgacgatca aatatgacgc tttggaggta
     1321 cagagatgtt ttcaacaatt agccgacacc gattcctgtt gctgctgctg ttatttgtgt
     1381 tggtggtcgc cattgtgccc gtgcaggcgc aagaagagcc gaccgttagc cccgacgcgc
     1441 cgaaaaaatc ggctgtccgt aaggagttgc gccagatcgc cagcgatacc tgggactcct
     1501 ttgtggccat gacctatccc aacggcttgc cggccgacaa catcagcgcc accggcgtgc
     1561 gcgccggtta cagctcgccc accaatatcg ccagctatat ctggagcacc ctggtggctc
     1621 gcgacctggg gatcatcacc caaagcgaag cccgccagcg catcggccaa acgttgacga
     1681 cactggcgac gatggagcgc cacggcgaca gcggcatgtt ctacaactgg tatgacccgg
     1741 ccaccggcgc caagttgacg acatggccac cgtcgggcga cccggtctac cccttcctgt
     1801 ccacggtcga taatggctgg ctggccgcgg cgctgatcat ggtgaccaac gccgtgccac
     1861 cgttgcgcga cgaagcgcag gccatcctcg acgacatgaa cttcggcttc tactacgatc
     1921 caggagccgg gctattgcgc ggcggcttct gggacgccgt tccgccgggt tgttccatcc
     1981 agggcaacta tccgggccag ggggccgacg tttattacac ctgccaccac tacggcacgc
     2041 tcaacaccga gccgcgcatc gccagctaca tcggcatcgc ctggggccag gtgccgccga
     2101 cgcactactt caagatgtgg cgcaccttcc ccgacacctg cgactggagc tggccggaga
     2161 tgaagcccga gggcgtgacg cgcaactatc tgggcgtgga cgtttacgaa ggacactata
     2221 cctaccgcgg catggacatc gtgcccagtt ggggtggcag catgttcgag gcgctcatgg
     2281 tcgagctgat ggtaccggag gctgagtggg ggccgaacag ctggggcatc aaccacccgc
     2341 tctacgtccg ggcgcagatc gagcacggcc tggaagaggc gcagtatggc tactggggct
     2401 tctcgcccgc caccaatccc tctggtggct accgcgagta cggcgtcgac gctattggca
     2461 tgaacaccga cggctacgcc tccaacaacg acaataccta cgtcgattac ggcttcggcg
     2521 attgccgccc cggccagccc attccgccgg cctctgccta taccaacggc gtcgtgacgc
     2581 cccacgctgt cttcctggcg ctggagtttg cgcccggcgc cacgttgcga aatatgcgca
     2641 atctgcgccg cgacttcgac gtttacagcg attggggctt ctatgactcg gtcaacgtcg
     2701 ataccgggca ggtatcgccc tactatttgg ccctcgacca gggcatgatc atggccgcgc
     2761 tgggcaacta tctgcgcaac gaccggttgg agttctactt cacccacggc gaggtacgcc
     2821 aggccatccg gccgctgctg gcaatggaac ggtttaccgc cggcgactag acccacgatc
     2881 cacacgacct gacaggtggc cgccacctgt caggtcgctt catccagcgg cggggggctt
     2941 aacggagacg ccatgttgct gcccaacgac tacggccact tcagcgacga cggccgcgaa
     3001 tacatcatca cccgtcccga tacccccatg ccgtggatca acgtcctgac caacggcgac
     3061 tatggcctga cgctgtcgca gacgggcggc ggctacagtt ggcgcggtca cgcctcgctc
     3121 aaccgtctga cgcgctggtc gcaagacctg gtgtgcgacg actggggtaa gtacctctac
     3181 atccgcgacg cggacagtgg cgacttctgg tcggcggcct ggcagcccgc ggggcagttg
     3241 ctggccggct accgcgtgcg ccacggtttc ggctacacca ctatcgagtg gcagcgcgac
     3301 acgctgggcg gcgcgttgac gacgtttgtg ccgttgggcg atccctgcga actgtggctg
     3361 ttgcgcctgg aaaatcacgg cccgcgttcg cggcggctgc aactgtttac ctatttagag
     3421 tggctgctgg gcgcggctcc cgattggcac cgcgaattcc atcgcctgtt catcgagtcg
     3481 cgctacgacg ccggccacca cgccctactg gccaacaagg tattgtggga gctgccgggg
     3541 ctgcccgggc cgcacctcaa ccggagttgg ccctacgtcg ctttccacag cagttcgcgg
     3601 cccccggctg gctacgacgg cgacaaggcc gccttcctgg ggcgctatgg gcgcacagcc
     3661 gcgccgcggg cggtgcgcga ggggcgctct ggcgacacgg ccggccgctg gggcgacgcc
     3721 atcgccagcc tgcacgtgcc gctcaccatg gctccgagcg aaacggtcga gatcgtcttt
     3781 gctctggggg cggccgacga tgccccggca gcgctggccc tcagcgacaa gtaccgctcc
     3841 ccggcggccg cccacgccgc gctagacgag gcgcgccgct tctgggccgg cctggtcgat
     3901 ggcctggccg tgaccacgcc cgacccggcg ctcgatgtca tggccaacgg ctggctggtc
     3961 tatcaggctg tggccggccg gctgtggggg cgctcggcct actaccagac gggcggagcc
     4021 tatggcttcc gcgaccagtt gcaggacagc ctcgtctggt tgctgcttga ccggccggcc
     4081 gacacgctgg cccagatccg cctccacgcc gctcaccagt atcaggaggg catcgtcctc
     4141 cactggtggc acccgctggc cgagacgggc ctgcgttcgc gctacagtga cgacctgctg
     4201 tggctgccgt tcgtcgtctg gcacaccatt caggagacgg gcgacctggc tttcctggat
     4261 gaggaggtgc cgttctacga tggtggcgcg gcttcgctgt gggagcattg ccagcgggcg
     4321 ttcgatgtgg ccctggcgcg gcgtagcccg cgcggtctgc cgctcatcct ggagggcgac
     4381 tggaacgacg gcctgaacgc caccggcccg gccggccgtg gcgaaagcgt ctggatggcc
     4441 cacttccttt actatctgct gacctgctgg gcgactctgc ccatcgacgc ggctacgcgc
     4501 gcgcgtttca cggccgaggc ggcggctctc cgcgccgcta ccgagcagca cggctgggat
     4561 ggggcgtggt attggcgggc gacgaccgac gaggggctgc aattgggcgc ggcggcgcgg
     4621 gtcgaggggc gcatcttcct caacgcgcag acgtgggcca cgctgagcgg cctggccgcg
     4681 cccgaccgca cccaacaggc gctggccgcc gcccgccaac acctctacac cgactacggc
     4741 gcgctgctgc tcttcccggc ctatagcgtg cccgaccccg acgtgggcta cctgacccgc
     4801 tatgcgccgg gcacgcgcga gaatggcggc gtctatgccc atgccgcctg ctgggccgtg
     4861 ctggccgagc ggcaggcggc tggggccggg gcggcctatg acctgtggcg gcgcatgtgc
     4921 ccgccgcgcc gcgccgccgc gcccgacgtc tacgccgccg agccatacgt catgcccggc
     4981 aacgtcaacg ggccgctggc tccgcggccg gggcagggcg gatggacgtg gtataccggc
     5041 gcggcggcct ggtacctgcg cgctctggtc gagggggtac tgggcgtgtc agccgggctg
     5101 gacgggctga gcgtggccgg cgacctgccc gccgagtggg acggcttccg tctgcggcgg
     5161 cgcttccgcg gcgcgaccta cgatatcgac gtccaccgcg ccgcagccgg cgcgcttgtg
     5221 gccggctgct gctgcgatgc tggcggc
//
LOCUS       DAOSMQ010000206         5215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_15998_length_5215_cov_4.897481, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000206 DAOSMQ010000000
VERSION     DAOSMQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15998_length_5215_cov_4.897481"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..2035
                     /locus_tag="PK829_08060"
     CDS             245..2035
                     /locus_tag="PK829_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU41205.1"
                     /translation="MATKLEFIGTLPIFANLSEAARAAVAAVATEYSFKPNAVIAYQR
                     DVANSLYIVKEGRLFAKGLDASGRAREGLTRYYEPGQSFNDLWLFVPGVHPATVKGST
                     GARGTSAPDDEGRLLIIPGPAFHKLLEEYPIIIDQLAPRDDGETHYGLSDAAWQEARK
                     MKLRRQVRASSAAALMPEERLEFFARRSVWLLVGRLAMPVVLVLAAVIILVLMPTNTG
                     LQRALKVGGPLALTLTGLVWFFLRVIDWRGDYFVITNRHMTHHEFDLRHFRIRLVKIP
                     IGQVQTVEVLKPTFLANLFNFGTARVTTAAAAGKVLFDFIDQPGRVKDVLERLLGQYR
                     AVESAQTQALMRQSIEKHFGVDSPLKLADAGGPAPARARPRPVGFFARLGRRYGWRVE
                     EGNTITYRKSLFVLLKRIAAPVLVLVAVIGLSILALYLDVIPWVVVLAAVVFGFGDLL
                     WLIWQIEDWRNDIFQLTDRFVIDIDRSPFGFGESRKQAPISNIQNVNADRPGFLPTLF
                     NYGFVSIDTAGAAADIVFEYVPNPEVIQSDIFQRLDEFRQQQRVREGSARREEYALLI
                     DVYRQAMEQQRIPQRTPSGTSEEESNQPAP"
     gene            2032..2607
                     /gene="def"
                     /locus_tag="PK829_08065"
     CDS             2032..2607
                     /gene="def"
                     /locus_tag="PK829_08065"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU41206.1"
                     /translation="MSKLPIVTIPDKILRQKTQPVTRFDADLQRLIDNMIATLREANG
                     VGLAAPQIGRPIQLAVILTLPEEDDEGNEIEDTRELYVIINPEIFWRSKEMVKGIEGC
                     LSIPGYVGEVERHESIRVRGQDRHGRKVRLRLYDWTARIFQHEIDHLNGELYIDKLTG
                     PENIWTEEEFEKMREAKQKARDETEEPMVGD"
     gene            2607..2783
                     /locus_tag="PK829_08070"
     CDS             2607..2783
                     /locus_tag="PK829_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOU41207.1"
                     /translation="MAGEEQRSSYVNNARAVTRRRGRAAQHLAGPFGERVFLYHNGAP
                     SFYAARGFRGSLRV"
     gene            2840..3130
                     /gene="gatC"
                     /locus_tag="PK829_08075"
     CDS             2840..3130
                     /gene="gatC"
                     /locus_tag="PK829_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOU41208.1"
                     /translation="MTLTLADVTHIAHLARLALTDEELAHYQSQLSAVLDYAARLNEL
                     DLTGVAPSTHAAGRRNVLREDVVAPSLAPDDALFNAASTAEGQFLIQSVLDA"
     gene            3130..4590
                     /gene="gatA"
                     /locus_tag="PK829_08080"
     CDS             3130..4590
                     /gene="gatA"
                     /locus_tag="PK829_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOU41209.1"
                     /translation="MDPSTLTELRDALRRGETTSVAATTAMIDRIVAYDNTLRSYLAV
                     ADESALEQAAAADRRRAAGEDGPLLGIPLAIKDIICTAGIPTTAGSKILEGFIPPYDA
                     FVVARLLAAGAVILGKTNTDEFAMGSSTENSAYVTTANPWDVDRVPGGSSGGSAAAVA
                     AGLAYGALGTDTGGSVRQPASFCGLVGLRPTYGRVSRWGVIAFASSLDQVGPFGRAVA
                     DCAALLGVIAGHDPRDSTSLDAPVPDYEAALTGDIRGLRVGVPREYFIEGIAPEVEAA
                     VRAAIDDLAALGAEVVEVSLPHTHYGLPVYYLIAPAEASANLARYDGTRFGPRVEGAD
                     MIDTVRQTRALFGPEVKRRIMLGTYALSAGYYDEYYGRALRARTLIAGDFRRAFETVD
                     VIAAPTSPTTAFRIGERVNDPLSMYLADVFTLPLNLSAACGLSVPCGVDSAGLPIGLQ
                     LIGDTLQEARILNAAYAYEQARPWHRRLPVVPALEP"
     gene            4792..>5215
                     /locus_tag="PK829_08085"
     CDS             4792..>5215
                     /locus_tag="PK829_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOU41210.1"
                     /translation="MFCGCRVVDSVTAAPNTAVCPVCLGMPGMLPVINRRAVEFALRA
                     ALALGCEVQAHNLFARKNYFYPDLPKGYQVSQYELPIGRHGRLDIALPDGTTKTIGIR
                     RVHMEEDTGKLTHRERGGSLVDYNRSGVPLLEIVSEPDI"
BASE COUNT          920 a         1826 c         1605 g          864 t
ORIGIN      
        1 ggccctggtc agtggctctt gccgccactc gtcccagttg cggacgatct ggatgacgag
       61 tagaaaggcg cggccgatgt acagcagggc aaggacgttg aatagaccgg taatgaccga
      121 catcagcaga ccagtacgat catcatagcc caccatacgc tgagcggcaa actgcgacca
      181 gacaatcctc agggagccat ccctccgttc gtcaatcggc cgccgttcat gctaaaattg
      241 ccctgtggct accaagctgg agttcattgg cacgttaccg atcttcgcca acctgagcga
      301 ggccgcccga gcggccgtcg cggccgtggc cacagagtac agctttaagc ccaacgccgt
      361 catcgcctac cagcgcgacg tggccaatag cctctacatc gtcaaggagg gtcgcctctt
      421 tgccaagggg ctggacgcca gcggccgcgc ccgcgagggc ctgacgcgct actatgagcc
      481 ggggcagtcc ttcaacgatc tgtggctgtt cgtgcccggc gtccacccgg ccacagtcaa
      541 aggctcgacc ggcgcgcgcg gcacgtccgc ccccgacgat gaaggccgcc tgctgatcat
      601 ccccggcccc gccttccaca agctgctgga ggagtacccg attatcatcg accagctggc
      661 cccccgcgac gacggcgaga cgcactatgg cctgagcgac gctgcctggc aggaggcgcg
      721 caagatgaag ctgcgccggc aggtgcgcgc ttcctcggcc gccgccctga tgcccgaaga
      781 gcggctggag ttcttcgccc ggcgcagtgt gtggctattg gttggccgcc tggcgatgcc
      841 ggttgtgctc gtgttggcag ccgtcatcat cctggtgctg atgcctacca acacagggct
      901 gcaacgggcg ctcaaggtgg gcgggccgct ggcgctgacg ctgacgggcc tggtgtggtt
      961 cttcctgcgc gtgatcgact ggcggggcga ctacttcgtc atcaccaacc gccacatgac
     1021 ccaccacgaa ttcgacctgc gccacttccg catccgcctg gtgaagattc ccatcggcca
     1081 ggtgcagacc gtcgaagtgc tgaagccaac cttcctggcg aatctcttca actttggcac
     1141 ggcccgcgtg acgaccgccg ccgcggcggg caaagtgctg ttcgacttca tcgatcagcc
     1201 cggtcgcgtc aaggatgtgc tggagcggct cctggggcag tatcgcgccg tcgaatcggc
     1261 gcagacgcaa gcgctcatgc gccagtcgat tgagaagcac tttggcgtcg attcgcccct
     1321 gaagctggcc gacgccggtg gccccgcgcc cgcgcgcgcc cgaccacggc cagtaggctt
     1381 cttcgcccgc ctggggcgac gctacggctg gcgcgtcgaa gagggcaata cgatcaccta
     1441 tcgcaagagc ctcttcgtgt tgctcaagcg aatcgccgcg cccgtgctgg tgctcgtcgc
     1501 cgtgattggc ctgagcatcc tggctctgta tctggatgtc atcccatggg ttgtagtttt
     1561 ggccgccgtc gtcttcggct tcggcgacct gctgtggctc atctggcaga tagaagactg
     1621 gcgcaatgac atcttccagt tgaccgatcg cttcgtcatc gacatcgacc gcagtccctt
     1681 tggcttcggc gagagccgca agcaagcgcc catcagcaac atccagaacg ttaacgccga
     1741 ccggcccggc ttcctgccga cgctgttcaa ctatggtttc gtcagcatcg acaccgccgg
     1801 cgcggcggcc gacatcgtct ttgagtacgt gcccaacccc gaagtcatcc agagcgacat
     1861 cttccagcgc ctggacgagt ttcgccagca gcagcgcgtc cgcgagggca gcgcccgccg
     1921 cgaagagtac gccctgctca tcgacgtcta ccgtcaggcg atggagcaac agcgcattcc
     1981 ccaacgcact ccgtccggca cttcagaaga agagagcaat cagccagcgc catgagcaaa
     2041 ctgcctattg tcacgattcc cgacaagata ctgcgccaga agacccaacc cgtcacgcgc
     2101 ttcgacgccg acttgcagcg cctgatcgac aacatgatcg cgacgctgcg cgaggccaac
     2161 ggcgtcggcc tggccgcgcc acagatcggc cggcccatcc aactggccgt catcctgacc
     2221 ctgcccgaag aggacgacga gggcaatgag atcgaggaca cccgcgagct gtatgtcatc
     2281 atcaacccgg agatcttctg gcggtcgaag gagatggtca agggcatcga gggctgcctg
     2341 tccatccccg gctatgtggg cgaggtggag cggcacgagt cgatccgcgt gcgcgggcag
     2401 gaccgccacg gccgcaaagt gcgtctgcgc ctctatgact ggacggcgcg catcttccag
     2461 cacgagatcg accacctcaa cggcgaactc tacatcgaca agctgaccgg cccggagaac
     2521 atctggaccg aagaagaatt tgagaagatg cgtgaagcca aacagaaagc cagagatgaa
     2581 acagaggagc cgatggtggg cgactagtgg ccggcgaaga gcaacggagt agttatgtca
     2641 ataacgcgag agctgtcacc aggcgacgag gaagagctgc tcagcacctt gcaggcccgt
     2701 ttggagaacg tgtcttcctg taccacaacg gcgcgccctc cttctatgcc gccaggggct
     2761 tccgcgggtc gctcagggtt tagctcactc atatcgacaa cggttcactg atccataacc
     2821 attgccctac cgacggccta tgactctcac ccttgccgac gtcacccaca ttgcccacct
     2881 ggcccggctg gccctgacgg acgaggaact ggcccactac cagagccaac tctcggccgt
     2941 gctcgactac gccgcccgtc tcaacgaact agacctgacc ggcgtggccc cctcaaccca
     3001 cgccgccggc cggcgcaacg tgctgcgcga ggacgtggtt gctccgtcgc tcgcgcccga
     3061 cgacgccctt ttcaacgctg cctcgacggc cgagggccag ttcctcatcc agtccgtcct
     3121 cgacgcctga tggacccatc aaccctgacc gaactgcgcg acgccctgcg ccgcggcgaa
     3181 acgaccagcg tggcggcgac gacggcgatg attgaccgca tcgtggccta tgacaacacg
     3241 ctgcgcagct acctggccgt ggccgacgag agcgccctgg agcaggccgc cgccgccgac
     3301 cgccgccgcg cggccggcga agatggcccc ctgctgggca tccccctggc catcaaggac
     3361 atcatctgca ccgccggcat ccccaccacg gccggcagca agattctgga gggcttcatc
     3421 ccgccctatg acgccttcgt cgtcgcgcgg ctgctggccg ccggggcggt cattttgggc
     3481 aagaccaaca ccgacgaatt cgccatgggt tcctccacgg agaactcggc ctacgtgacc
     3541 accgccaacc cctgggatgt ggaccgcgtg cccgggggca gcagcggcgg cagcgccgcc
     3601 gccgtggccg ccgggctggc ctatggcgcg ttgggcaccg acaccggcgg cagcgtgcgc
     3661 cagccggcat cgttctgtgg cctggtgggg ctgcggccga cctatggccg cgtgtcccgc
     3721 tggggcgtga tcgccttcgc ctcctcgctc gatcaggtgg ggccattcgg ccgcgccgtg
     3781 gccgactgcg ctgccctcct gggtgtcatc gccggccacg acccgcgcga cagcacgtcc
     3841 ctcgacgcgc ccgtgcccga ctacgaagcc gccctgaccg gcgacatccg cggtctgcgc
     3901 gtcggcgtgc cgcgcgaata cttcatcgag ggcatcgccc cggaggtcga ggccgccgtg
     3961 cgcgccgcca tcgacgacct ggcggcgctg ggggcggagg tcgttgaagt gtcgctgccc
     4021 cacacccact acggcttgcc ggtctactac ctcatcgccc cggccgaggc cagcgccaac
     4081 ctggcccgct acgacggcac gcgctttggc ccgcgcgtcg agggggccga catgatcgac
     4141 accgtccgcc agacgcgcgc cctgttcggc ccggaggtta agcggcgcat catgctgggg
     4201 acgtacgctc tcagcgcggg ctattacgac gaatactatg gccgggcgtt gcgggcgcga
     4261 acgctcattg ccggggactt ccgccgcgcc tttgagacgg tcgatgtcat cgccgcgccg
     4321 accagcccaa caactgcctt ccgcatcggc gagcgcgtca acgacccgtt gagcatgtat
     4381 ctggccgatg tcttcaccct gccgctgaac ctgagcgccg cctgcggtct gtccgtgccc
     4441 tgtggcgtcg atagcgccgg gctgcccatc ggcctgcaac tcatcggcga cacgttgcag
     4501 gaagcgcgca tcctcaacgc cgcctacgcc tatgagcagg cgcgaccgtg gcatcgccgg
     4561 ctgcccgttg tgcccgctct ggagccatag ccggccgaat cttttcacgt tgcgtccacc
     4621 gccggcgcaa cccaaaggag gtacaattga ttcagcgcca agcgcggcgt atacttggcg
     4681 cgcggcccgg cctcgacaga gcgctgagca aacgagggaa agcgagtggg tgagcgctat
     4741 gaagccgtca tcggcctgga gatccacgcc gaactgctaa ccgagtcgaa gatgttctgc
     4801 ggctgccgcg ttgtcgacag cgttaccgca gcgcccaata cggccgtctg cccggtctgt
     4861 ctgggtatgc cggggatgct gccggtcatc aaccggcgcg ccgtcgagtt tgccctgcgc
     4921 gccgccctgg cgctgggttg tgaggtgcaa gcccacaacc tcttcgcccg caagaactac
     4981 ttctaccccg acctgcccaa gggctaccag gtcagccagt acgagctgcc catcggtcgc
     5041 cacggccggc tagatatcgc cctgcccgac ggcacgacca agaccatcgg catccgccgc
     5101 gtccacatgg aagaggacac cggcaagctg acccaccgcg agcgcggcgg ctcgctggtt
     5161 gattacaacc gctccggcgt gcccctgctg gagattgtct ccgagccgga catcc
//
LOCUS       DAOSMQ010000207         5196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16079_length_5196_cov_4.335927, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000207 DAOSMQ010000000
VERSION     DAOSMQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16079_length_5196_cov_4.335927"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK829_08090"
     CDS             <1..271
                     /locus_tag="PK829_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HOU41211.1"
                     /translation="GLTLTDSFMPANAGRTVVDVDGGRASLRTGAAGVEIGMDIAEFS
                     SLVVGAVDFARLYEYGLATIDDPGAVALVTQLFRAAQPPWCLTHF"
     gene            complement(371..2407)
                     /locus_tag="PK829_08095"
     CDS             complement(371..2407)
                     /locus_tag="PK829_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOU41212.1"
                     /translation="MTNRLAHEASPYLRQHADNPVDWYPWGEEALRAAREQDKPILLS
                     IGYAACHWCHVMAHESFEDPATAAQMNRDFINIKVDREERPDLDSIYMGAVVAMTGQG
                     GWPMTVALTPDGQPFFGGTYFPPTPRHGMPAFRQVLTSLADAWRDRRADVEGSAADVA
                     AHLRQTTLAAALGGGGALNADLLDQALNALLRSFDARLGGFGGAPKFPPSMTLEFLLH
                     VTAQRGDSMALHMAERTLEAMAHGGLYDQIGGGFARYSTDDRWLVPHFEKMLYDNALL
                     ARVYTHAWQITGKPLYRRVVEETLDFVAREMRHEAGGFSSSYDADSEGEEGKFYVWTA
                     DEIRAALGDEAGRFMAAYGVSEAGNWEGRNILHVAEDLSGGDHLTGLAEARRTLFEIR
                     ARRVPPGLDDKALTAWNGLMLAAFADAGQALGRPDYIAVAVANADFLRARMRRDDGRL
                     WRAWQPGLGDAPGRFNAYLEDYACLADGLLALYAATFEPRWFAWARELADAMLAHFSD
                     PAGGFFDTSDDHEDLLHRPKDVQDNATPSGNSMAAHVLLRLGLLTGEGRYWDAAEGLV
                     SSLYTAMARYPSAFAHWLDAAAFILGEPREVAIIGDPAAADARALLDVVLGRYRPDLV
                     VAAGEGEAAAVVPLLAGRGRLDGRAAAYVCRRFVCQQPVSEPDALASQLGWGRG"
     gene            complement(2515..3327)
                     /gene="trpA"
                     /locus_tag="PK829_08100"
     CDS             complement(2515..3327)
                     /gene="trpA"
                     /locus_tag="PK829_08100"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOU41213.1"
                     /translation="MPNTPTETTTGLETIAAAFAATRRAGRAALMPYFTLGYPDRATS
                     LDIIAAIGADSDLLELGVPFSDPLADGPTIQHSTQVSLEGGTTLAGCLDMLRALRGRG
                     VTTPVLLFGYYNPFLAYGLPALAADARAAGAQGFIVPDLPPEEAADFEAVAAAEGLAL
                     IHFLAPTSSPHRIETVTARAHGFIYLVSVTGVTGARAGLHADLAGFIGRVRAQTTTPL
                     AVGFGISTPEQAATIGRLADGVIVGSALINAVDAASDDKPAAAARFVRSLRA"
     gene            3487..4338
                     /locus_tag="PK829_08105"
     CDS             3487..4338
                     /locus_tag="PK829_08105"
                     /inference="COORDINATES: protein motif:HMM:NF018234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU41214.1"
                     /translation="MKQIALILLILILAGCGRANEPPAGPPTAASDATEALVATNVPA
                     TTGAEAATARPTDAVTGAVGSAEQVSFEAADGLLIRGTLLLPLTSGADPGVILLHMLG
                     DDRTVWGEVGLAADLVAAGYAVLAVDMRGHGETGGAADWTLAADDLARVWDAFVALDS
                     VDDARTAVIGASIGANMALRLGVDRPDIRAVVALSPGLDYRGVTTAELPAAYGDRPLL
                     LVASEDDAYAADSVRALAESAATARVEVYATAGHGTNMFAAAPELTPLIIGWLDEQLP
                     SPQQRTP"
     gene            4427..4795
                     /locus_tag="PK829_08110"
     CDS             4427..4795
                     /locus_tag="PK829_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOU41215.1"
                     /translation="MSVFGNIIWLIFGGFLAGLGYILGGLLLCLTIVGIPFGVQAIHL
                     GMATFAPFGKEVVAKPRGEGCLALGFNVLWIVLFGWEIAIAHLIGAALLAITIIGLPF
                     AKQHLKLIPVSLAPFSYDLK"
     gene            4881..>5196
                     /locus_tag="PK829_08115"
     CDS             4881..>5196
                     /locus_tag="PK829_08115"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOU41216.1"
                     /translation="MSDLILTQQRDSLFEISLNRPEKRNAINLEMLRQFDAAVGQANR
                     TPGLRAVVIRGEGSVFSAGIDVSALLGLAETYGPHWQQRMRAITDEFQSVLTRLERLE
                     LPT"
BASE COUNT          769 a         1724 c         1879 g          824 t
ORIGIN      
        1 cggcctgacg ctgaccgaca gcttcatgcc cgccaacgcc ggccgcacgg ttgtggatgt
       61 agatggcggg cgggcctcgc tacggacggg cgcggccggc gtggagatcg gcatggatat
      121 cgccgagttc tcgtcgctgg tcgtcggcgc ggtcgatttt gcccggctgt atgagtacgg
      181 gctggcgacc atcgatgacc ccggcgcggt ggcgctggtg acgcaactat tccgcgccgc
      241 gcagccgccg tggtgcctga cgcacttcta gggcgtgtag gcaaaggacg cgccccggcg
      301 gtgtaggggc tagggattag gggctagggg ctggggaaga gcgctctctc tagcccctat
      361 cccctagccc ctagccccta ccccagccca gttggctggc cagcgcgtcc ggctcgctga
      421 ccggctgttg gcaaacgaag cgccggcaga cgtaggccgc ggcccggccg tccagccgcc
      481 cgcgcccggc cagcagcggc acgacggcgg ccgcctcgcc ctcgccggcg gcgacgacca
      541 gatcaggccg gtagcgcccc aggacgacat cgagcagcgc ccgcgcgtcg gctgccgccg
      601 ggtcgccgat gattgccacc tcgcgcggct cgcccaggat gaacgccgcc gcgtcgagcc
      661 agtgggcaaa ggcgctgggg tagcgggcca tcgctgtgta aaggctgctg accagcccct
      721 cggccgcgtc ccagtagcgc ccctcgccgg tcagcaggcc cagccgcagc aggacgtggg
      781 cggccatgct gttgccgctg ggggtggcgt tgtcctgcac gtctttgggg cggtggagca
      841 ggtcttcgtg gtcgtcggag gtgtcgaaga agccgcccgc cgggtcgctg aagtgggcca
      901 gcatagcgtc ggccagttcg cgcgcccagg caaaccagcg cggctcgaag gtggccgcgt
      961 agagggccag caggccatcg gccaggcagg cgtagtcctc caggtaggcg ttgaagcggc
     1021 cgggcgcgtc gcccaggccg ggctgccagg cgcgccacag tcggccgtcg tcgcggcgca
     1081 tgcgggcgcg caggaaatcg gcgttggcca cggccacggc gatatagtcg ggccggccca
     1141 gcgcttgccc ggcgtcggcg aaggcggcca gcatcaggcc gttccaggcg gtcagcgcct
     1201 tgtcgtccag gccgggcggc acgcggcggg cgcggatctc aaagagcgtg cggcgggcct
     1261 cggccagacc tgtcaggtgg tcgccacctg acaggtcttc ggcgacgtgg aggatgttgc
     1321 gcccctccca gttgcccgcc tcgctgacgc cataggcggc catgaaccgc ccggcctcgt
     1381 cgcccagcgc ggcgcgaatc tcgtcggccg tccagacgta gaacttgccc tcctcacctt
     1441 cgctgtcggc gtcgtagctg ctgctgaaac cgcctgcctc gtggcgcatc tcgcgggcga
     1501 cgaagtcgag cgtctcctcg acgacgcggc ggtagagcgg cttgccggtg atctgccagg
     1561 catgggtgta gacgcgggcc aggagggcgt tgtcgtagag catcttctcg aagtggggca
     1621 cgagccagcg gtcatcggtc gaatagcggg cgaagccgcc gccgatctgg tcgtacagac
     1681 cgccgtgggc catcgcttcc agagtgcgct cggccatgtg cagggccatg ctgtcaccgc
     1741 gttgcgccgt gacatgcagc aggaattcca gcgtcatcga cggcgggaac ttgggcgcgc
     1801 cgccgaagcc gcccagccgc gcgtcgaagc tgcgcagcag ggcgttgagg gcctggtcga
     1861 gcagatcggc gttcagcgcg ccgcccccgc ccagcgccgc ggccagcgtc gtctggcgca
     1921 ggtgggccgc cacgtcggcg gcgctgcctt ccacgtcggc gcggcggtcg cgccaggcgt
     1981 cggccaggct ggtcagcacc tgacggaagg cgggcatgcc gtggcgcggc gtgggcggga
     2041 agtaggtgcc gccgaagaag ggttggccgt ccggcgtcag ggccacggtc atcggccagc
     2101 cgccctggcc ggtcatggct acaaccgcgc ccatgtagat gctgtccagg tcgggccgct
     2161 cctcgcggtc gaccttgatg ttgatgaagt cgcggttcat ctgcgccgcc gtggccgggt
     2221 cttcaaagga ctcgtgggcc atcacgtggc accagtggca ggcggcatag ccaatcgata
     2281 gcaggatggg cttgtcctgc tcgcgggcag cgcggagggc ctcttcgccc caggggtacc
     2341 agtccaccgg gttgtcggcg tgctggcgca ggtaggggct tgcttcgtga gccagtctat
     2401 ttgtcatagt gtgagcttat cacaaacgca gtcggaacct cacgccaagc cgcaaggaac
     2461 accaggctca caattctgag cttggcgttc tctgcgtctt tgcgtgagat tttcttatgc
     2521 gcgaagagaa cggacgaagc gggcggcggc ggccggtttg tcgtcacttg ccgcgtccac
     2581 ggcgttaatc agcgcgctac cgacgatgac gccgtcggcc agccggccga tggtcgccgc
     2641 ctgctccggc gtgctgatgc cgaagccgac ggccagcggc gtagtggtct gcgcgcgcac
     2701 gcggccgatg aagccggcca ggtcggcgtg tagcccggcg cgcgccccgg tgacgccggt
     2761 gacgctgacc agatagatga agccgtgggc gcgcgcggtc acggtctcga tgcggtgggg
     2821 gctgctggtg ggggccagaa agtggatgag cgccagcccc tcggccgcgg cgacggcctc
     2881 gaagtcggcc gcttcctccg gcggcaagtc gggcacgatg aagccctgcg ccccggcggc
     2941 gcgagcatcc gcggccagcg cgggcagccc gtaggccagg aaggggttgt aatagccgaa
     3001 gagcagcacc ggcgtcgtca caccgcgccc gcgcagcgcg cgcagcatgt ccaggcagcc
     3061 ggccagcgtc gtgccgccct ccagtgagac ctgggtgctg tgctggatgg tcgggccgtc
     3121 ggccagcggg tcgctgaagg gcacgcccag ctccagcagg tcgctgtcgg cgccgatggc
     3181 ggcgatgatg tccagcgagg tggcccggtc ggggtagccg agggtgaagt agggcatcag
     3241 ggcggcgcgg ccggcgcggc gcgtggccgc aaaggccgcg gcgatcgttt ccagtccggt
     3301 ggtcgtctcg gtcggcgtgt tgggcatggg accgcattat agaccaatgc cggcggtggt
     3361 gcgattctcc gaatcgcgtt ctgcgacacc acaaggatgc gattctggag aatcgcgcca
     3421 cgcgggagca agtctgccgg gttgggcgct ataatgacga cagcatgccg agaggtaggt
     3481 aaaaggatga aacagattgc gctcatcttg ttgatcctga ttctggccgg ctgcggccgg
     3541 gcgaacgagc cgccggccgg gccgcctacg gccgcgtccg atgccactga agcgcttgtg
     3601 gccaccaacg tgcccgcgac cacaggggcc gaagccgcga ccgcgagacc gaccgatgcc
     3661 gtcactgggg cggttggttc ggcggaacag gtgtcgttcg aggcggccga cggcctgctc
     3721 atccggggca cgctcctgct gccgctgacg agcggggccg atccgggcgt catcctgctc
     3781 cacatgctgg gcgacgaccg caccgtctgg ggtgaggttg ggctggcggc cgatctggtc
     3841 gcggccggct acgctgtgct ggcggtggac atgcgcggcc acggcgagac gggcggcgcg
     3901 gccgactgga cactggccgc cgacgatctg gctcgcgtct gggatgcctt tgtcgccctc
     3961 gacagcgtcg atgatgcgcg cacagccgtc atcggggcca gtatcggcgc gaatatggcc
     4021 ctgcgactgg gcgtcgaccg gccggacatc cgcgccgtcg tggccctatc gccggggctg
     4081 gactatcgcg gcgtgaccac ggccgagttg ccggcggcct atggcgaccg gccgctgctg
     4141 ctggtggcca gcgaggacga cgcctacgcg gctgacagcg tgcgggcgct ggccgagagc
     4201 gccgccaccg cgcgcgtgga ggtgtatgcg acggccggcc acggcacgaa catgtttgcc
     4261 gccgcgccag agctgacgcc actgatcatc ggctggctcg acgaacagct accgtccccc
     4321 caacagcgca caccctagtg aaacgtttgg gccgcccctg cgtactgttc tctgagaaaa
     4381 catgggctgc tccggcaaga agggcagccg cacgataagg aataacatga gtgtcttcgg
     4441 caacatcatc tggctaatct tcggtggctt tctggccggg ctgggctaca tcctgggcgg
     4501 gctgctgctg tgcctgacca tcgtcggcat tcccttcggc gtgcaggcca tccacctggg
     4561 catggccacc tttgcgcctt tcggcaagga agtcgtcgcc aagccgcgcg gcgagggctg
     4621 cctggcgcta ggcttcaatg tcctgtggat cgtgctcttc ggctgggaga tcgccatcgc
     4681 ccacctgatc ggcgcggcgc tgctggccat caccattatc ggcctgcctt tcgccaagca
     4741 gcacctgaag ctgattccgg tgtcgctggc gccattcagc tacgacctga agtaattccc
     4801 ggcgggcgca aggcttcgcg gccggccttg cgcccgcgct ttcctactcg gccgcatcgc
     4861 tctatggtac agtccgggcc atgagcgacc tcattctcac ccagcaacgc gatagtctat
     4921 tcgagatcag cctcaaccgg ccggagaagc gcaacgccat caacctggag atgctgcgcc
     4981 agttcgacgc agccgtgggc caggccaacc gcacgcccgg cctgcgcgcc gtggttatcc
     5041 gcggcgaagg cagtgtcttc tcggccggca tcgacgtcag cgccctgttg gggctggccg
     5101 agacgtatgg cccccactgg cagcagcgca tgcgggccat caccgacgag ttccagagcg
     5161 tgctgacgcg gctggagcgg ctggaactgc cgacca
//
LOCUS       DAOSMQ010000208         5143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16309_length_5143_cov_4.709119, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000208 DAOSMQ010000000
VERSION     DAOSMQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16309_length_5143_cov_4.709119"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK829_08120"
     CDS             complement(<1..225)
                     /locus_tag="PK829_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41217.1"
                     /translation="MTWQQSLRLFLSPRPDGQLLRLAIVAVGQTLRGDDGVGPALARR
                     LAGQPAADAWLLLDAGHAPENFLGPIVRFRP"
     gene            complement(179..598)
                     /locus_tag="PK829_08125"
     CDS             complement(179..598)
                     /locus_tag="PK829_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU41218.1"
                     /translation="MRIGLLRDLSRSAFRRPVTERYPFEKRPAPPNYRGKLHWTAEGC
                     TGCNLCVKDCPAEAIDFFILDKANKQFIFRYHIDRCTFCGQCAFNCRFNCITLANEEW
                     ELACGDRDAFIVTYGRPEDIARLDLATESAAIPESAA"
     gene            complement(595..1542)
                     /locus_tag="PK829_08130"
     CDS             complement(595..1542)
                     /locus_tag="PK829_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOU41219.1"
                     /translation="MALDLLNLLFFPAGLGLIGAGLLYQWADRKLLARFQNRLGPRWF
                     QPFADALKLLGKEEIHPAGSSPVLFAFLPVLALSGALTAALYVPLFGLAPVFSFPGDL
                     IVTLYLMSLLTLCIGLAGAQTRDRFSLAGALRVFTQMFAYEAPFILALLTPAVALRLA
                     GQPGSWQISELATAAAGRPWFLLLLPITFVVALVGLMGKLELPPFDAPEAETEIVAGA
                     LTEYSGRGLALFSLARAVELVVGLTLVTALYLGGVGSGPLAAVLFTAKTVGLLLVIVV
                     LQTLLARLRIDQTVNLWWRIGSVLVLAQWLLLILYQVLR"
     gene            complement(1548..2774)
                     /locus_tag="PK829_08135"
     CDS             complement(1548..2774)
                     /locus_tag="PK829_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOU41220.1"
                     /translation="MNASASDRFVIPIGPQHPALKEPGHFEFTVEGETVIDATVRLGY
                     AHRGIEKGAEGRNWTQSLYLLERICGICSHIHSTAYVVGVEQMAEVEVPARAQAIRET
                     IAELERIHSHMLWLGVAAHDAGFDSLFMYTWRDRETVMDLLEAISGNRVHYSANSLGG
                     VKFDLSDETAAAILKGIDFLEERTRHYREVVSQDAFFLQRTRGVGTMTRQEALQLGVV
                     GPTARASGVARDVRIDSPYIAYNEFPAHIQLLSAGDLEARFLVRIDEMLDCYRLIREL
                     LARMPAGELAAKRMPRKLKPGEVISRVEAPRGELFYYIRSTGGEQPDRVKVRTPTLCN
                     MGSVLTQTIGHNLADVPMILVGIDPCFSCNDRAVSVRRAGRAEGQPWSWADLRRYGID
                     FYRRQMGLETHALAKG"
     gene            complement(2771..3250)
                     /locus_tag="PK829_08140"
     CDS             complement(2771..3250)
                     /locus_tag="PK829_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOU41221.1"
                     /translation="MTLTQKPLERVEALLEPWLTRTERPADNRLDIWLPADDLLPVVA
                     TLVEDEWGYLAAITGLDPGVATGELHVLYHFAEGAAVVTLRVAVPRDEAEVPTIRPLI
                     PLAAIYEQELSEVLGVAIIGAPARGRLFLPDDWPAGVYPLRKDFQLEQLDDKEQGGS"
     gene            complement(3216..3785)
                     /locus_tag="PK829_08145"
     CDS             complement(3216..3785)
                     /locus_tag="PK829_08145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOU41222.1"
                     /translation="MGIELPPRWRTALDRLRNWSLANSPWLVHYNSGSCNGCDIEILA
                     TLTPRYDLERLGIKLQGSPRHADVLVCTGPVTRQSRDRLRRIYEQMPEPKFVVAVGAC
                     SISGGAFRGCYNILGGVDEVIPVDVYVPGCAPRPEAIINGVAILLKKLQPDRATEIDR
                     LLVEAVGDTAVLTEEEVGDDAYAEALGAR"
     gene            complement(3822..4157)
                     /locus_tag="PK829_08150"
     CDS             complement(3822..4157)
                     /locus_tag="PK829_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41223.1"
                     /translation="MSALLTPPIALLAYVGLVALLLVFGRLLAPAGRRQAAEVLYAGG
                     EEAATTKAAPGYRRFFVVALFFAVLHLGALVLATAGLIGGALGAGFSTALLIYLGGLA
                     LALVILLLS"
     gene            complement(4187..>5143)
                     /locus_tag="PK829_08155"
     CDS             complement(4187..>5143)
                     /locus_tag="PK829_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU41224.1"
                     /translation="ILSVRAALASAPVAPVLWLTAGALFVIGFGIKVALVPLHTWLPD
                     AHAQAPSGISAILSGVVIEAGLIALLRALSGLAGAAVSWGALLIGLGLFNIVVGNLMA
                     LRQQQVKRLLAFSSISHVGYMLLGLGIALYTGTAAGAQAGFFHLLSHGLMKGLAFLAI
                     GALMFGLQHSLGQAADAGGHAPLTVDDLAGAARRYPLVALVFSLAVLGLGGLPPLVGF
                     MSKWQVLVAGAQTRDTFILVVLLIAALNSVLSLGYYAPLVNRLYRREPGALVAAGRPI
                     PAAMLAPLVVLAVAIVLLGVLPMVAGRLTIPAAADLLAIFAP"
BASE COUNT          960 a         1681 c         1760 g          742 t
ORIGIN      
        1 aggccggaag cggacgatag ggccgaggaa gttctccgga gcgtggccgg cgtcaagtag
       61 cagccaggcg tcggccgccg gctggccggc cagccggcgg gccagggccg gccccacgcc
      121 atcgtcaccg cgcagcgtct gcccaacggc gacaatggcc agccggagta gctgcccgtc
      181 aggccgcgga ctcaggaata gccgcagact ctgttgccag gtcaaggcgg gcaatatcct
      241 ccggccggcc ataggtgact atgaaagcgt cgcggtcgcc gcaggccagt tcccactcct
      301 cgttggcgag ggtaatgcag ttaaagcggc agttgaaggc gcactgaccg cagaaggtac
      361 agcggtcgat atgatagcgg aagatgaact gcttgttggc cttgtcgaga atgaagaagt
      421 cgatggcctc ggccgggcag tccttgacgc acaggttgca accggtgcag ccctcggccg
      481 tccaatggag cttgccgcgg taattcggcg gcgcgggccg cttctcgaat gggtagcgct
      541 cggtcaccgg gcggcggaag gccgaccggg acaggtctct gagcaggccg attctcatct
      601 tagcacctgg tacagaatga gcagtagcca ctgggccagc acgaggaccg acccaatgcg
      661 ccaccacagg ttgaccgtct gatcgatgcg cagacgggcc agaagtgtct ggagcacaac
      721 gataaccagc agcagaccga cggtcttggc cgtgaaaaga acggccgcca gcggcccgct
      781 gcccacgcca cccaggtaga gcgccgtcac cagcgtcagg ccaacaacca gttccaccgc
      841 gcgggccagc gagaacagcg ccaggccgcg cccactgtac tccgtcaacg ccccggcgac
      901 gatctccgtc tccgcctctg gcgcgtcgaa ggggggcagc tctagcttgc ccattagccc
      961 aacgagggcg acgacaaagg tgattggcag caagagcagg aaccaggggc ggccggccgc
     1021 ggctgtggcc agttcgctga tctgccagct accaggttgc ccggccaacc tcagggcgac
     1081 ggccggtgtc agcagggcca ggatgaacgg cgcttcgtag gcgaacatct gcgtaaagac
     1141 gcgcagcgcc ccggccagcg agaagcggtc gcgcgtctgt gccccggcga ggccgatgca
     1201 cagcgtcagc aggctcatga ggtagagtgt gacgatgagg tcgccgggga agctgaagac
     1261 gggagccagg ccgaataggg gcacgtaaag cgccgccgtc agcgccccgg acagagccag
     1321 cacaggcagg aaggcgaaga gcacggggct gctgccggcc gggtgaattt cctccttgcc
     1381 caggagcttc agcgcgtccg cgaaaggttg gaaccagcgc ggccccagcc ggttctggaa
     1441 gcgggccagc agcttgcggt cggcccattg gtagagcaac ccggccccaa tcaggcccag
     1501 cccggccggg aaaaagagca ggttgagcag gtctagtgcc atgttggcta tcccttcgcc
     1561 agcgcgtggg tctcaaggcc catctgccgg cgatagaagt cgataccata gcgccgcagg
     1621 tcggcccacg accagggctg gccttcggct cgcccggcgc ggcgcacgct gacggcgcgg
     1681 tcgttgcagg agaagcacgg gtcgatgccc acgaggatca tgggcacgtc ggccagattg
     1741 tggccgatgg tctgcgtgag cactgaaccc atgttgcaca gcgttggcgt gcgcaccttt
     1801 acccggtcgg gctgttcgcc gccggtgctg cggatgtagt agaacagttc accgcgtggc
     1861 gcttcgacgc ggctgatgac ttcgccgggc ttcagcttac gcggcatgcg cttggcggcc
     1921 agttcgccgg cgggcatacg ggccaacagt tcgcggatga ggcggtagca gtcgagcatc
     1981 tcgtcgatgc ggacaaggaa gcgcgcttcc agatcgccgg cggacaggag ttgaatgtgg
     2041 gcggggaact cgttataggc gatgtagggc gaatcgatgc gcacgtcgcg ggccacgcca
     2101 gaagcgcgcg ccgttggccc gacaacgccc aattgcagcg cttcctggcg cgtcatcgtg
     2161 ccgacgccgc gcgtgcgctg caagaagaag gcgtcctggc tgacgacctc gcggtagtgg
     2221 cgcgtgcgtt cttccaggaa gtcgatgccc ttcaggatgg ccgcggccgt ctcgtcactc
     2281 aggtcgaact tgacgccgcc gaggctgttg gccgaatagt gaacgcggtt gccgctgatg
     2341 gcctccagca aatccatgac cgtctcgcgg tcgcgccagg tatacatgaa cagcgagtcg
     2401 aagccggcgt cgtgggcggc cacgcccagc catagcatgt ggctgtggat gcgctccagt
     2461 tcggcgatgg tctcgcggat ggcctgggcg cgggcgggca cttccacctc ggccatctgc
     2521 tccacaccga caacataggc ggtgctgtgg atgtgggagc agatgccaca gatgcgctcc
     2581 agcagataga ggctctgggt ccagttgcgc ccctcggccc ccttctcgat gccccggtgg
     2641 gcgtagccca ggcgcaccgt ggcgtcgatg acggtctcgc cctcgacggt gaactcgaag
     2701 tggcccggct ccttcagtgc cgggtgttgt gggccaatgg gaatgacaaa gcgatcgctg
     2761 gcggaagcgt tcatgaccca ccctgctcct tgtcgtcgag ctgctccaac tggaagtcct
     2821 tacgcaacgg gtagacgccg gcgggccagt cgtcgggcag gaagagccgg ccgcgtgccg
     2881 gcgcgccgat gatggccacg cccagcactt cgcttagctc ctgctcgtat atggccgcca
     2941 gcgggatcag cggccggata gtcggcactt cggcttcgtc gcgtggcacg gccacgcgca
     3001 gggtgacgac ggccgcgccc tcggcgaagt ggtagagcac gtgcaactcg cccgtggcca
     3061 cgccggggtc cagcccggtg atggccgcca gatagcccca ctcgtcctca accagcgtgg
     3121 ccacgaccgg caacaggtca tcagccggca gccagatgtc gagccggttg tcggccggcc
     3181 gctcggttct ggttagccac ggctctagca gcgcctcaac gcgctccaag ggcttctgcg
     3241 taagcgtcat cgcccacctc ctcttccgta agaacggccg tgtcgccaac ggcctcaacc
     3301 agcagccggt cgatttcggt cgcgcggtcg ggctgcaact tcttcagcag gatggccaca
     3361 ccgttgatga tggcctccgg ccgcggggcg cagccaggga cgtagacatc gacggggatg
     3421 acctcatcga cgccgccgag aatgttgtag cagccccgga aggctccgcc ggagatgctg
     3481 cacgcgccca cggcgacaac gaacttcggc tctggcatct gctcatagat gcgccgcaaa
     3541 cggtcgcgcg attggcgcgt caccgggccg gtgcagacga gcacgtcggc gtggcggggg
     3601 ctgccctgga gcttgatgcc caaccgttcc aggtcgtagc gcggcgtcag cgtagccagg
     3661 atttcgatgt cacagccgtt gcacgagccg ctgttatagt gaaccaacca gggcgagttg
     3721 gccagcgacc agttgcggag gcgatccagg gcggttcgcc agcggggcgg caattcgata
     3781 cccattctga tgtccatagc gggcctgttc cctcagctat ctcaactcag caacaaaatg
     3841 accagcgcca gcgccagccc gcccaggtag atgagcaggg ctgtgctgaa gcccgcaccc
     3901 agagcgccgc cgatcagccc ggctgtagcc aacaccagcg cgcccaaatg gagcacggca
     3961 aaaaacaggg cgacgacgaa gaagcgccgg tagccaggcg cggccttggt tgtggccgcc
     4021 tcctcaccgc cggcgtagag cacctcggcc gcctggcgac gcccggccgg cgccagcagc
     4081 cgcccgaaga ccaacagcag ggccaccagg ccaacgtaag ccagcagggc aatgggtggg
     4141 gtcagaagcg cactcattct gatgtccata acgttacctc aagaccttac ggcgcgaaga
     4201 tggccagcag atcggccgcc gccgggatgg tcagacgccc ggccaccatt ggcaagacgc
     4261 ccagcaggac gatggccacg gccaggacga ccaacggcgc cagcatggcc gccggaatgg
     4321 gccggccggc ggccaccaac gcgcccggtt cgcgccggta gagccggttg accagcggcg
     4381 cgtagtaacc cagcgatagg acgctgttca gcgcggcgat gagcaggacg acgaggatga
     4441 acgtatcccg tgtctgcgcc ccggcgacga gcacctgcca cttggacatg aagccgacca
     4501 gcggcggcag cccgcccagg ccgagcaccg ccaggctgaa caccagcgcc accagcgggt
     4561 agcgccgcgc cgccccggcg aggtcatcga cggtcagggg ggcgtggccg ccggcgtcgg
     4621 cggcctggcc cagcgagtgc tgcaagccga acatcagcgc gccgatggcc aaaaaggcca
     4681 gacccttcat caggccgtgg ctcagcaggt ggaagaagcc cgcctgcgcc ccggcggccg
     4741 tgcctgtgta gagggctatg cccaggccga ggagcatgta gccaacgtgg ctgatgctgg
     4801 agaaggccaa tagtcgcttc acttgctgct ggcgcagggc catcaggttg cccacgacga
     4861 tgttgaacag gcccaagccg atcagcagcg cgccccagga cacggccgcc ccggccagcc
     4921 cggacagtgc ccgcagcaga gcgatgagtc cggcctcgat gacaacgcca gagagaatgg
     4981 cgctgatgcc actgggagcc tgcgcgtggg cgtcgggtag ccaggtgtgc aggggcacga
     5041 gggcgacctt gatgccgaag ccgatgacga acaacgctcc ggctgtcagc cacagcaccg
     5101 gggccaccgg cgcgctggcc agcgccgccc gcacgctgag gat
//
LOCUS       DAOSMQ010000209         5140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16323_length_5140_cov_4.668830, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000209 DAOSMQ010000000
VERSION     DAOSMQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16323_length_5140_cov_4.668830"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK829_08160"
     CDS             <1..248
                     /locus_tag="PK829_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41225.1"
                     /translation="ITAAGGETGDVTLPDAAGTPTAGPDGIAQTDATPVAIDATPDAT
                     EEGTASATAFGVGPCAAALLLPLAVVVSRGAARRRRA"
     gene            complement(319..984)
                     /locus_tag="PK829_08165"
     CDS             complement(319..984)
                     /locus_tag="PK829_08165"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOU41226.1"
                     /translation="MSPPPCTFDDVRAALALPGFDGAAAQRLMAPVLRPMSRPEGLAS
                     PPRLGAVLILLYCADDRLTLVLTQRPEYEGVHSGQVSCPGGRHEPPESLGATALRETY
                     EEIGVPPAEVELLGELTPLYIMPSDFQVTPFVGRYVGPGRPRFVPDTREVAAILEVPL
                     DLLLDPATRAEEEMELRGGLRLDVPFFRVNEGRVWGATAMMLSEFLARLRAARAGQAA
                     AGG"
     gene            complement(992..1747)
                     /locus_tag="PK829_08170"
     CDS             complement(992..1747)
                     /locus_tag="PK829_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU41227.1"
                     /translation="MSEQSTRQQRILAIDDNAYTLRIVEHTLRDAGFRVTTAISGEEA
                     LAQIEREGLPDLAIADLHMPGISGFEFAHRVHQYSDLPIVMLTATGHEATVVEGLEEH
                     AEDYIVKPFSPGELVARVRRVLRRIGEFAYDLEPQTRIDERLLIDFPNRTAIVDGKSE
                     ALTPTETKLLYILMRKAGQTVPTDYILRRLWPMEPVFEDRLHVHLHRLRRKIEDKKDK
                     SRGRYIVSERGAGYSFRDLKHPRAVAQADDEDE"
     gene            complement(1754..2575)
                     /gene="pgeF"
                     /locus_tag="PK829_08175"
     CDS             complement(1754..2575)
                     /gene="pgeF"
                     /locus_tag="PK829_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOU41228.1"
                     /translation="MIAFDDDDLHYFRFAGLPANGTADHAVFSRHGGVSVGPFASLNL
                     SLSVPDARDNVYANRRRAYGLFGRDTDTVVHAHLVHGASVARVTTADNGTWVEHVDGL
                     ITDQPGCVLGMNYADCAPIFLYDPRRHAIGLGHAGWRGTVVDLPGAMVRAMTAHFGSD
                     PADLVAAIGPCIGSCCYEVGAEVIDAVRAAFVDAETLLSAQGDTRSRHFDLPAANRRN
                     LLNAGVAAIELAEVCTACRTDLFFSHRAERGRTGRFGTVFALGQPAGEPPHKVSI"
     gene            complement(2716..>5140)
                     /locus_tag="PK829_08180"
     CDS             complement(2716..>5140)
                     /locus_tag="PK829_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOU41229.1"
                     /translation="RKLREIILAAEISRTYEKDEILELYLNEIYYGNLAYGIEAAANT
                     YFGKSAADLTLAEASLLAGLPQAPALWDPYSAPDKALGRQREVLSLMVSNEDVTLEEA
                     QAALNDMAARVYTLQRPTVTIQHPHFTFTVLQQAEELLGAQSIYRGGLSIQTTLDPDA
                     QRLAEEAVAANRDALVAGGANNTALVALQPQTGEILAMVGSADFNDEAISGQVNMALA
                     PRQAGSSIKPLVYLSAFERGWTPATLLWDVQTQFPDGVNPPYIPKNYDDEFHGPLRVR
                     PALGNSYNIPAIKALEFVGVCEFIANVQKVGLTALQDSGCAETGQPRDHGLALALGSG
                     EIPPLEMAGAFGALANGGRYIQPYAIRRIEDRQGNVLYDYAPPDATAAQVVRPEHAYL
                     LTSILSDNNARLAEFGQTNLLDIPGYQVAVKTGTSGSTASDVRDGWTIGYTPQALAAV
                     WIGNTDNSPVAAGQSGYRLAAPIWNQFMTQYLAGKEALTFPRPAGVVEREICADTGIA
                     VSATTPCAQRLVEVFAADQPPLDETQGTTRVSIDLWTGLRANEFCNDAVYEATFGGGV
                     PVNGRPEVLERERTLAQQWLQTAGQGWSGQSSGGVSFGTPPAAACDANTPRPRAEIAR
                     PREDRDIPDRREVRITGTAMGPNFAGYQVEYGLGQNPGGWGLIQERRPDAVQDDLLAV
                     WDTTQIDYSGPVTIRVIVFGPDNPNTPENDPVMKEGRTVVNLVQPTATATTTPTETPT
                     ATATLQPTATPTLFPTATATLPPTVTLPPSATLPPAATVPPSATPVEELPSPTPAPPG
                     TTPEATPYP"
BASE COUNT          719 a         1706 c         1891 g          824 t
ORIGIN      
        1 gcatcaccgc cgcgggcggc gagacgggcg acgtgaccct gccggacgcg gccggcacgc
       61 cgacggccgg ccccgacggc atcgcccaga cggacgcgac tccggtggcc attgacgcca
      121 cgccggacgc gaccgaagag ggcacagcca gtgccaccgc ctttggcgtc gggccgtgcg
      181 ccgcggcgct gctgctgccg ctggctgtcg tcgtcagccg gggcgcggcc cgccgccggc
      241 gcgcgtagcc ggcgcgcctg tccagaaacg acgcgggcgc tcacgggagc gcccgcgttg
      301 ttgtctgtct gggccggttc agccgcccgc ggcggcctgc ccggcgcgcg cggcccgcag
      361 ccgcgccagg aactcgctca gcatcatcgc cgtcgccccc cagacccgcc cctcgttgac
      421 gcggaagaag ggcacatcga gccgcaggcc gccgcgcagc tccatctcct cctcggcccg
      481 cgtggccggg tcgagcagca ggtcgagcgg cacttccagg atggcggcca cttcgcgcgt
      541 gtcgggcacg aagcgtggcc gccccgggcc aacgtaacgg ccgacaaagg gcgtcacctg
      601 gaagtcggac ggcatgatgt agagcggcgt caactcgccc agcaactcta cctcggccgg
      661 ggggacgccg atctcctcgt aggtctcgcg cagggcggtc gcgcccagcg attcgggcgg
      721 ctcgtggcgt ccgccggggc atgacacctg gccggaatgg acaccctcgt actccggccg
      781 ttgggtcagg acaagggtca gccggtcgtc ggcgcagtag agcagaataa gcaccgcgcc
      841 cagccgcggc ggcgaggcca ggccctccgg ccgggacatc ggccgcagca cgggggccat
      901 cagtcgttgc gccgccgcgc cgtcgaagcc gggcagcgcc agcgcggcac gaacgtcgtc
      961 gaaggtgcag ggcggcgggc tcacgccggg tctattcgtc ctcgtcgtcg gcctgggcca
     1021 cggcgcgggg gtgcttcagg tcgcggaagc tgtagcccgc gccgcgttcg gagacgatat
     1081 agcggccgcg cgacttgtcc ttcttgtcct cgatcttgcg gcgcagccgg tggaggtgga
     1141 cgtgcaggcg atcctcgaaa accggttcca tcggccagag gcgacgcagg atgtagtcgg
     1201 tcggcacggt ctggccggcc ttgcgcatga ggatgtagag cagctttgtc tctgtcggcg
     1261 tcagggcctc gctcttaccg tcgacgatgg cggtgcggtt gggaaagtcg atcagcagcc
     1321 gctcgtcgat gcgcgtctgc ggctccaggt cataggcgaa ttcgccgatg cgtcgcagca
     1381 cgcgccgcac gcgggccacc agctcgcccg ggctgaacgg cttgacgatg tagtcctcag
     1441 cgtgctcctc cagcccctcg acgacggtgg cctcgtggcc ggtggccgtc agcatgacga
     1501 tgggcaggtc gctgtactgg tggacgcggt gggcaaactc gaagccgctg atgccgggca
     1561 tgtgcaggtc ggcaatggcc aggtcgggca agccctcgcg ctcgatctgg gccagggcct
     1621 cctcgccgga gatggccgtc gtgacgcgaa aaccagcgtc gcgcagggtg tgctctacga
     1681 tgcggagtgt gtaggcgttg tcgtcgatgg ccaggatgcg ctgttgtcga gtggattgtt
     1741 cgctcataat ccgtcagatc gataccttgt ggggcggctc cccggctggt tggcccaggg
     1801 caaagaccgt cccgaagcgc cccgtccggc cgcgttcggc gcggtgggaa aagaacagat
     1861 cggtgcggca ggcggtgcaa acctccgcca gctcgatggc cgcgacgccg gcgttgagca
     1921 ggttgcgccg gttggccgcc ggcaggtcga agtggcggct ccgcgtgtcg ccttgcgccg
     1981 acagcagcgt ctcggcatcg acgaaggccg cgcgcacggc gtcgatgacc tccgcgccaa
     2041 cctcatagca gcatgagccg atgcacgggc caatggcggc gacgagatcg gccgggtcgc
     2101 tgccgaagtg ggcggtcatg gcccgcacca tcgcccccgg cagatcgacg accgtgcccc
     2161 gccagccggc gtggcccagg ccgatggcgt ggcggcgcgg gtcgtagagg aagatgggcg
     2221 cgcagtcggc gtagttcatg cccagcacgc agccgggctg gtcggtgatc aggccatcga
     2281 cgtgctcgac ccaggtgcca ttgtcggccg tggtcacgcg ggcgacgctg gccccatgca
     2341 ccagatgggc gtggacgacg gtgtcggtgt cgcggccaaa gaggccataa gcacgccgcc
     2401 ggttggcata gacgttgtcg cgcgcgtcgg gcaccgatag gctcagattg agtgaggcga
     2461 atgggccgac actaacgccg ccgtgccggg aaaagacagc atggtcggct gttccgttgg
     2521 cgggcaagcc ggcaaagcga aagtagtgca agtcgtcgtc gtcgaaagcg atcacaggcg
     2581 tcctcgtccg tcccttaggc tacttcaaag cggcccggtg gacaaatcag gcgggcaaaa
     2641 accggccggg ttctcatacg aacccggccg gtttttttct gttctggcgc gcctctggcg
     2701 cgccgtccga cgggcctacg ggtaaggtgt tgcctcaggc gtggtgccgg gcggcgcggg
     2761 cgtcgggctg ggcaactcct ccactggtgt ggcgctgggc ggcacggtgg ccgccggcgg
     2821 cagcgtggcg ctgggcggca gcgtaacggt cggcggcagc gttgccgtcg ccgtcgggaa
     2881 gagcgtcggc gtggccgtcg gctggagggt ggctgtggcc gtgggtgtct ccgttggcgt
     2941 cgtcgtggct gtggccgtcg gctgcaccag gttgaccacc gtgcgcccct ctttcatcac
     3001 cgggtcgttc tccggcgtgt tggggttgtc cggcccgaag acgatgacgc ggatggtgac
     3061 cgggccgctg tagtcgatct gcgtcgtgtc ccagacggcc agcaggtcgt cctggacggc
     3121 gtccggccgc cgctcctgga tcaaccccca accaccgggg ttctggccca ggccatactc
     3181 cacctgatag ccggcgaagt tcggccccat ggccgtgcca gtgatgcgca cctcgcgccg
     3241 gtcggggata tcgcggtctt cgcgcgggcg ggcaatctcc gcccgcggcc ggggcgtgtt
     3301 ggcgtcgcag gccgctgccg gcggcgtgcc gaaggacacc ccgccgctgc tctggcccga
     3361 ccagccctgt ccggcggtct gaagccactg ctgggccagc gtccgctcgc gctccaacac
     3421 ctccggccgg ccgttgaccg gcacgccgcc gccgaaggtg gcctcgtaga cggcatcgtt
     3481 acagaactcg ttagcccgca gccccgtcca caggtcgatg gacacccggg ttgtgccctg
     3541 cgtctcgtcc agcggcggct ggtcggcggc gaagacctcg accaggcgct gggcgcaggg
     3601 cgtcgtggcg ctgacggcga tgcccgtgtc ggcgcagatc tcccgctcga caacgccggc
     3661 cggccgcggg aaggtcagcg cttccttgcc ggctaggtat tgggtcatga actggttcca
     3721 gatgggcgcg gccagccggt agcccgactg cccggcggcc accggcgagt tgtcggtgtt
     3781 gccgatccac accgctgcca gcgcctgcgg cgtgtagccg attgtccagc cgtcgcgcac
     3841 gtcgctggcc gtcgagccgg aggtgccggt cttgacggcg acctggtagc cggggatgtc
     3901 cagcaggttc gtctggccga actcggccag gcgagcgttg ttgtcgctga ggatgctggt
     3961 cagcaggtag gcatgttccg ggcggacgac ctgagcagcc gtggcgtcgg ggggggcgta
     4021 gtcgtagagg acgttgccct ggcggtcttc gatgcggcgg atggcgtagg gctggatgta
     4081 gcgcccgccg ttggccagcg cgccaaaggc tccggccatc tccagtggcg gaatctcgcc
     4141 gctgcccagg gccagcgcca ggccgtggtc gcgcggctgg cccgtctcgg cacagccgct
     4201 gtcttgcagc gccgtcaggc cgaccttctg gacgttggcg atgaactcac acacgccgac
     4261 gaactccagc gccttgatgg cgggaatgtt ataggagttg cccagcgccg ggcgcacgcg
     4321 cagcgggccg tggaactcgt cgtcgtagtt cttggggatg tagggcgggt tgaccccgtc
     4381 ggggaactgc gtctgcacgt cccagagcag cgtcgccggc gtccagccgc gctcgaaggc
     4441 ggacaggtag accagcggct tgatgctgga gccggcctgc cgcggggcca gggccatgtt
     4501 gacctggccg ctgatggctt cgtcgttgaa gtcggcgctg ccgaccatgg ccagaatctc
     4561 gcccgtctgc ggctggaggg ccaccagggc ggtgttgttg gccccgccgg ccaccagcgc
     4621 gtcgcgattg gcggcgacgg cctcctcggc caggcgctgc gcgtcggggt cgagcgtcgt
     4681 ctggatgctc aggccaccgc ggtagatcga ttgcgccccc agcagctcct cggcctgctg
     4741 caagacggtg aaggtgaagt gtggatgctg gatggtgacc gtcggccgct gcaacgtgta
     4801 gacgcgggcg gccatgtcgt tcagtgccgc ctgggcctcc tccagggtga cgtcctcgtt
     4861 ggagaccatc aggctgagca cctcgcgctg gcggccgagg gccttgtcgg gcgcgctgta
     4921 agggtcccac agcgccgggg cttgcggcag cccggccagc aacgacgcct cggccaacgt
     4981 caggtcggcg gccgacttgc cgaagtaggt gttggccgcc gcctcgatgc cataggccag
     5041 gttgccatag taaatctcgt tgagatacag ctccagaatc tcgtccttct cgtaggtacg
     5101 cgagatttcg gcggccagga tgatctcgcg cagcttgcgc
//
LOCUS       DAOSMQ010000210         5136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16350_length_5136_cov_4.839402, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000210 DAOSMQ010000000
VERSION     DAOSMQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16350_length_5136_cov_4.839402"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK829_08185"
     CDS             <1..476
                     /locus_tag="PK829_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41230.1"
                     /translation="TISPTATLTSTLVATPTMTPTITMTPTPTATPPLPTAEPDIATT
                     TPTPTPTATKEPRLPACLRINFDVSGQEAKRGLYVAQEVGGHFLAAWEADDGWKDSGW
                     FYDIDITFPAVYVDVLYYHGPGVEPVRLKMLNPAPDVDAGWVARGQCHALEVGWP"
     gene            complement(514..1713)
                     /locus_tag="PK829_08190"
     CDS             complement(514..1713)
                     /locus_tag="PK829_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU41231.1"
                     /translation="MGPEELRFLLSAEGQAVLAELAATPVSEGTHMAVAARLRAQLGP
                     ERGRAALDTALLRQRAARKFSRAAAMMFTRDGLEQATAEPIAAHRAARFAAAGVTTVA
                     DLGCGIGGDALALAGAGAAVLAIDRDRLRLLMAAANAAVYDVAGRLALAQADLLTLPP
                     ARVEAFFFDPARRAGGGPREAPSRRLRAVADYQPPLSLIDAWRPVVPRGAVKVAPGIE
                     DAEVPAGVEVEIVSLGGEVKEAVLWYGELRTAARRATLLPGGQTLSGEADVAGAAPVA
                     APRAYLYEPDGAVIRAHLVGLLAAQLDATLLDPHIAYLTTGAPRPTPFARLFAIDDAF
                     PFQLKRLRAALRARRVGRVTIKKRGSPLEPEALRQALRLRGPNEAIVFLTQVMGRHTV
                     LIGRSLA"
     gene            complement(1717..2535)
                     /locus_tag="PK829_08195"
     CDS             complement(1717..2535)
                     /locus_tag="PK829_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU41232.1"
                     /translation="MTDRLTLSQNHLYTFRACPRRFYLRYLARVPWPEAPLGRSQELA
                     YERGQRFHRRLERHFLGLPTASDEETDAELRVWWDTFGSHAPALPEGRRFVEAGLTVP
                     IGNHFLTGRFDLLIVGAGPDGQPAAHLFDWKTGEPRPLDRLRAAWQTRVYLAVLAEGG
                     AALLPETAAAFAPDRLSLTYWYVADPEQPRVIAYDAAAHRRNWAELESIVAAIDSLLP
                     AGTADAWPLTDDLSECRRCAYQALTGRLAAGEAGPEALDEDEPPPDDWLEPQWG"
     gene            2711..3952
                     /locus_tag="PK829_08200"
     CDS             2711..3952
                     /locus_tag="PK829_08200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU41233.1"
                     /translation="MHVFLRPRRAAVLALTLLIVLAACAAPAVEVAPAAEASAAQPTT
                     AAQPTTAAQPTTAPALPPRAGPTTATLPTAAPSLTPRPTATPPATPTTLPSPTVAPTP
                     TEPATPTADAATGLPCPDPAPVKPDYLAYHLSAAPWPVPDAAASRPPLSLANPLPNAG
                     RNSGYPYGSDGSGRYLLHNGLDMADENRDLAVAPADGQVIVARDDIDEMFGWRCDWYG
                     QLVVLRLDETQDGQPVYVLFGHVQDVQVTEGQRVARGEPVARQGTAGVATVAHLHLEV
                     RVGANAFGATRNPLLWLEPWSGSGVVAGRLVAPDGHAWEGVTVTLIDNAAEASYLTTW
                     TYLDDPDHLIRPDPALAENFVFGPVAEGSYDVFVTLQEETYRQRIEVEDGALTTVEIV
                     TEPYRTPTPTPPASPGATPSP"
     gene            complement(3949..5040)
                     /locus_tag="PK829_08205"
     CDS             complement(3949..5040)
                     /locus_tag="PK829_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOU41234.1"
                     /translation="MPTRNKPTWMLYGAYGFTGRLILAEAVRRGHRPVLAGRNAAKLA
                     ALATQYDLDWLALDLHYAERLHKTVERFSLVFHAAGPFVRTSEPMVRACLAGQTHYID
                     ITGEIPVFESTFAHDADARARGVVLMSGAGFDIVPSDCLARHVAERAPGATQLQTAVA
                     MPLGQVSSGTVKSGLVNANRFAGGSLTRRDGRLMPLPLGKERRKVRFSDGHTREVTPF
                     PWGDLVTAAHTTGIDTITSYVALPRLPAMGLLARPALRLLGTNAAQRLADRAATMMFT
                     GPDEATQRDGRAYLWARAEGPEGATEAWLETGEVYRFTALAGVQIVERVLAAGKRGEA
                     LAGALTPAGALGADFVLGVEGVRRFDTLP"
BASE COUNT          736 a         1853 c         1830 g          717 t
ORIGIN      
        1 tgaccatctc gcccacggcg acgctgacga gcaccctggt ggccacgccg acgatgacgc
       61 ccacgatcac gatgacgccg acgccgaccg ccacgccgcc gttgccgacg gccgaacccg
      121 acattgccac gaccacccca acgccaaccc caacggcgac gaaagagccg cggctgccgg
      181 cctgcctacg catcaatttc gatgtcagtg gccaggaagc caagcgcggc ctgtacgtcg
      241 cccaggaagt tggcggccat ttcctggcgg cctgggaggc ggacgatggc tggaaggata
      301 gcggctggtt ctacgacatc gacatcactt tcccggcggt ctacgtggat gtgctctact
      361 accacggccc tggcgtcgaa cccgtgcgcc tgaagatgct caatcccgcg ccggacgtgg
      421 acgccggctg ggtggcgcgc ggccaatgcc atgccctgga ggtgggctgg ccgtaggccg
      481 tctggcggcc gggcgagcgc gccgcgccgg gcgctaggcc aggctgcggc cgatcagcac
      541 cgtgtgccgg cccatcacct gggtcagaaa gacgatggcc tcattggggc cgcgcagacg
      601 aagggcttgc cgcagagctt ccggctccag cggcgagcct ctttttttga tggtcacccg
      661 ccccactctc cgcgcgcgca gggcagcccg cagccgcttg agttgaaagg gaaaggcgtc
      721 gtcgatggcg aaaaggcggg cgaaaggcgt cggccggggc gcgccggtgg tcaggtaggc
      781 gatgtggggg tcgagcaggg tcgcgtctag ttgcgccgcc agcaggccga ccaaatgggc
      841 gcggataacg gctccgtccg gctcatagag gtaggcccgc ggggcggcaa cgggggccgc
      901 gccggcaacg tcggcctcgc cactcagcgt ctggccgccg ggcagcagcg tcgcccgccg
      961 cgccgccgtg cgcagctcgc cataccatag caccgcctcc ttaacctcac cgcctagcga
     1021 aacgatctcc acctccacgc ccgcggggac ttcggcatcc tcaatgcctg gagcgacctt
     1081 gaccgcgccg cgcggcacga cgggtcgcca ggcgtcgatc aggctcagcg gtggttggta
     1141 gtcggccacg gcgcgcagcc gccggctggg cgcttcgcgc ggcccgccgc cggcgcgccg
     1201 ggcggggtca aagaagaacg cctcgacgcg tgccggcggt agcgtcagca gatcggcctg
     1261 cgccagggcc agccgtccgg ccacgtcgta gacagcggcg ttggccgcgg ccatgagcag
     1321 gcgcagccgg tcgcggtcaa tggccaggac ggccgcgccc gccccggcca gcgccagcgc
     1381 gtcgccgccg atgccgcagc cgaggtcggc tacggtggtc acgccggcgg cggcaaagcg
     1441 ggcggcgcgg tgggcggcga tcggctcggc tgtggcctgc tccaacccgt cgcgcgtgaa
     1501 catcatcgcc gccgcgcggc tgaacttgcg cgccgcgcgc tggcgcagca gagccgtgtc
     1561 cagcgccgcg cgcccccgtt ccggccccag ctgggcgcgc aggcgggcgg cgacggccat
     1621 gtgcgtgcct tccgacaccg gcgtggcggc caactcggcc agcacagctt gcccctccgc
     1681 gctcagcagg aagcgcagct cctcaggccc catcgcctag ccccactgtg gctccagcca
     1741 gtcgtcgggc ggcggctcat cttcgtccag ggcctccggc ccggcctcgc cggccgccag
     1801 ccggccggtc agagcctggt aggcgcagcg acggcactcg ctcaggtcgt cggtcagggg
     1861 ccaggcgtcg gccgtgcccg ccggcagcag actgtcgatg gccgcgacga tgctctccag
     1921 ttcggcccag ttgcgccggt gggcagcggc gtcataggcg atgacgcgcg gctgctccgg
     1981 gtcggccaca taccagtagg tcaggcttag ccggtcgggc gcgaaagccg cggccgtctc
     2041 cggcaacagc gccgcgcccc cctcggccag cacggccagg tagacgcgcg tctgccaggc
     2101 cgcccgcagc cggtcgagtg ggcgcggctc gcccgtcttc cagtcgaaga ggtgggccgc
     2161 cggctgtccg tcagggccgg ccccgacgat gagcaggtca aagcggccgg tgaggaaatg
     2221 gttaccgatg ggcactgtca gcccggcctc gacgaagcgc cggccttcgg gcaatgccgg
     2281 ggcgtggctg ccgaaagtgt cccaccagac gcgcagctcg gcgtccgtct cctcgtcgct
     2341 ggccgttggc agacccagga agtggcgctc cagccggcga tggaagcgct ggccgcgctc
     2401 ataagccagc tcctgtgacc ggcccagcgg cgcttcgggc cagggcacgc gggccaggta
     2461 gcgcaaatag aagcggcgcg ggcaagcgcg aaaggtgtag aggtggttct gggagagcgt
     2521 caggcggtcg gtcatagctt cgctcaatgg gttcgggttc agttagtcgc cgccggctgg
     2581 gcatgggcgg ggcgacagcc gcatgataga ggatgttggc cgggcgggca acgcttgcgc
     2641 gcggttgacc ggctaagagt tggcccgcgc cggttgacgc cgccgggctg gcgcggcaga
     2701 atcagcggcc atgcatgtct tcctccggcc gcgccgcgcg gctgttctcg ctcttaccct
     2761 gttgattgtg cttgccgcct gtgccgcgcc ggccgtcgag gtcgcgccgg cggctgaggc
     2821 ctccgcggcc cagccgacca ccgcggctca gccgaccacc gcggctcagc cgaccaccgc
     2881 gccggcgctg ccaccgcgcg ccggccccac caccgccacc ctgcccacgg ccgcgccgtc
     2941 gctgacgccc cggccgacgg ctacgccccc ggccacgccg acgactttgc cgtcgccgac
     3001 ggttgcgccc acgcccacgg agccggccac gcccacggcc gatgctgcta ctggtctgcc
     3061 ctgccccgac cccgcgccgg tcaagcccga ttacctggcc tatcacctgt cggccgcgcc
     3121 ctggccggtg ccggacgccg ccgcgtcccg gccgccactg tcgctggcta acccgctgcc
     3181 caatgccggc cgcaacagtg gctaccccta cggctccgac ggcagcgggc gctatctgct
     3241 ccacaacggc ctcgacatgg ccgacgagaa ccgcgacctg gccgtggccc cggccgacgg
     3301 ccaggtcatc gttgcccgcg acgacatcga tgagatgttc ggttggcgct gcgactggta
     3361 tggccagctt gtggtactgc gactggatga gacgcaggac gggcagcccg tctatgtcct
     3421 cttcggccac gtgcaggatg tgcaggtcac ggaggggcag cgcgtggccc gcggcgagcc
     3481 ggtggcccgc caggggacgg ccggcgtagc caccgtggct catctgcatc tggaagtccg
     3541 cgtcggggcc aatgctttcg gggcgacgcg caacccgctg ttgtggctgg agccgtggtc
     3601 gggcagtggc gtcgtcgccg ggcggctggt ggcccccgac ggccatgcct gggaaggcgt
     3661 gacagtgacg ctcatcgaca acgctgccga ggcgtcttac ctgaccacct ggacatatct
     3721 cgacgacccc gaccatctca tccgccctga cccggcgctg gcggagaact tcgtctttgg
     3781 ccccgtggcc gagggcagct atgatgtttt cgtgacgcta caagaggaaa cgtaccggca
     3841 gaggatcgag gtcgaggatg gcgcgctgac gacagtggag atcgtcactg agccataccg
     3901 gacgccaacg cccacaccgc ccgcctcgcc cggagcgacg cccagccctt agggcagcgt
     3961 gtcgaagcgc cgcacgccct cgacgcccag cacgaagtcg gcccccaacg cgcccgccgg
     4021 cgtcagcgcg ccggccagcg cttcgccgcg cttgcccgcc gccagaaccc gttcgacgat
     4081 ctgcacgccc gccagcgccg tgaagcggta gacctcgccc gtctccagcc aggcctcggt
     4141 cgccccttca ggcccttcgg cgcgcgccca gaggtaggcc cggccgtcgc gctgcgtggc
     4201 ctcgtccggg ccggtgaaca tcattgtcgc cgcgcggtcg gccaggcgtt gggccgcgtt
     4261 cgtgcccaac agccgcagcg ccggccgggc cagcagcccc atcgccggca gccgcggcag
     4321 ggcgacgtag ctggtgatgg tgtcgatccc ggtcgtgtgc gccgccgtca ccagatcgcc
     4381 ccaggggaag ggcgttacct cgcgcgtgtg gccgtcgctg aagcgcacct tgcgccgctc
     4441 cttgcccagc ggcaggggca tcaggcggcc gtcgcgccgc gtcaggctgc cgccggcaaa
     4501 gcggttggcg ttgaccaggc ccgacttgac cgtgccggag gagacttggc ccaggggcat
     4561 ggccacggcc gtttgtaact gggtcgcgcc gggcgcacgc tcggccacgt ggcgggccag
     4621 gcaatcactg ggcacgatgt cgaagcccgc gccggacatg agcacgacgc cgcgggcgcg
     4681 ggcgtcggcg tcgtgggcga aggtgctctc gaagacgggg atttcgccgg taatgtcgat
     4741 gtagtgggtc tggccggcca ggcaggcgcg caccattggc tcgctggtgc gcacgaaggg
     4801 gccggcggcg tggaacacca ggctgaagcg ttcgaccgtc ttgtgcagcc gttcggcgta
     4861 gtgcaggtca agggccagcc agtcgaggtc atactgggtg gccagggcgg ccagcttggc
     4921 ggcgttgcgc ccggccagca ccggccggtg gccgcgccgc acggcctcgg ccaggatcag
     4981 ccggccggtg aagccatacg cgccatagag catccaggtg ggtttgttcc gcgtgggcac
     5041 agacagagaa taccacatct tggcctgtag cacagcctgt taggctgtcg tcgccggcca
     5101 cagcctgaca ggctgtgcta cgggcctgct gtagta
//
LOCUS       DAOSMQ010000211         5128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16391_length_5128_cov_4.436428, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000211 DAOSMQ010000000
VERSION     DAOSMQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16391_length_5128_cov_4.436428"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2560
                     /locus_tag="PK829_08210"
     CDS             <1..2560
                     /locus_tag="PK829_08210"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU41235.1"
                     /translation="LARSMAKSPAQSFATGAEMADALRSAFVALEGLPTRVMLHQELD
                     ILDQVAEPPAGFELQIQTPGHGDSLVPLTRAVITLGRNLDNDIVLPADGVSRHHTRLQ
                     ATSLGWEVVDLGGVNGTWLNEHRLRPEEPTPLPVGSVLKIGPYEMILQGPDAPVPVEA
                     DRGAPISAPVTPGLIAGRTTAPMAPTSSSPQLSSPGPTAEQPTSPPTQPPAPLAIHMA
                     RDKFTAEPGQRVEVKVDVENRGDQEDRVTLSVQGIDPSWVDVPEEFTVVPPRQTVSIP
                     FAIHPPRRRGTPAGRQRLRIDLKSQRYPDARVGATAALTVGPFVAFEASMHPTEVRLP
                     GRTVVTIHNTGNAPAAFNVNGSDLQQLVRFRGAREGIALQPNQTANVELVLEMRETNL
                     FGEEDAYPFEVEVSTGPTVKQVLPGEAIARSIIPTPWLYVLLFLIIFGGVLGGLALVS
                     NWGRFLGIGDVPTSTPTILPVQNDATQTAAAAVLTVALGTQSAAATNVAATAQVAGDT
                     DQDGLTNNQETALGLDANNPDSDADGLKDGDEVLIYTTNPKVRDSDNDLLTDFDEVNT
                     YKTNPNANDSDGDGIPDGLEVGQGTNPLVPEAPTVTPITPSPTTPPPSATWTPIASIT
                     PTWTPIPSATATWTPIPSATATWTPIPSLTPTATSTLPPLPALACVPGPINIDGVLNV
                     GTEWPAAPTFTFQPTVAGATRLVNVYAVRDAGRLYLGFLVNDPANELTDSVRLYIDTT
                     NNGGDGDTSDRFFQVARDGSRLLWAGIGSNADGQEWNSNYTSNNWTAAVGEPGNGQWV
                     VEIQIDAVAELGALGDPFGLMAQVLYTGDLASYPSTASSSQANTWQDIDNVLCP"
     gene            2768..4000
                     /locus_tag="PK829_08215"
     CDS             2768..4000
                     /locus_tag="PK829_08215"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41236.1"
                     /translation="MQQVAITDLSPRARARGLRTVLAVWFLMNAGFFLIIPLLSVHYV
                     DRLGWAAAFIGLVLAVRQFVQQGLTVFGGALADRVGPRRLILLGVLIRAASFIVMGFA
                     SVPWLLLLSGLLAALGGALFDAPIRATLATLAPEEELTAIYGRLGILQNVARTVGPLI
                     GAYLIRFDFQMVGLASAAFFLLAFVVTLLFLPPVSVCSEPQTVRAGLRLALGDRAFVV
                     FTLLMMGFWFMWVQLSIAMPLQIRALTGQDSSVGIMFTISAVLAIVLQAPALRVTNRY
                     LRSTTTIVLGVAVMALGMALIGLAPGLAAFYGGLFFFSLGTVLAMPNSQTVTADMSDD
                     RARGAYFGVSSLAMAIGGGLGHILGGTLVDMATTRGAPALPWLVFAGVGGATVIGLAL
                     FYWRQTPPAAFRLIQRPG"
     gene            complement(3981..5015)
                     /locus_tag="PK829_08220"
     CDS             complement(3981..5015)
                     /locus_tag="PK829_08220"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU41237.1"
                     /translation="MDMLAYLSPDRAVLLARLAAERAYLLQRLEGLDEATLTHDPVFD
                     GWTVAALLAHLAYWDALEADRLGKLADGRPGDIEPPGIGADTVDAHNAALQRRFAGLP
                     FSQGLALCQKENRSLMLALDRLPDALLFGRARLRPGWRATPATWLRRRARHDAGHAAD
                     LARWRAGYPPNDRSVRVIHRDLLRPLVGQSRAELLALIALIPPDARQSQAIEGEWSLP
                     QVLGHISDYDRLGVLALQRLAAGQTPDDEQTFDIEAYNVERGPFWTGVTLAEAWAQFV
                     ATRRALLALAATLDDATLARPFTAPWGAPTTACGYLLDMAGHDGEHADALRRSLGLPA
                     LPRRLIRGAG"
BASE COUNT          790 a         1734 c         1744 g          860 t
ORIGIN      
        1 gctggcccgg tcgatggcca agtcgcccgc ccagagcttc gccaccgggg cggagatggc
       61 cgacgctctg cgcagcgcct ttgtcgctct ggaggggctg cccacgcggg tgatgttgca
      121 ccaggagtta gacattctcg accaggtggc cgaaccgccg gccggctttg agttgcagat
      181 tcagaccccc ggccacggcg atagccttgt acccctgacg cgggcggtta tcactctggg
      241 gcgcaacctg gacaacgaca tcgtcctgcc ggccgacggt gtgtcgcgcc accacacgcg
      301 cctccaggcg acttcgctcg gctgggaggt ggtcgatctg ggcggcgtca acggcacgtg
      361 gctgaacgaa caccgcctgc ggccggagga gccgacgccg ctgccggtcg ggtcggtgct
      421 caagatcggg ccatacgaga tgatcctgca ggggccggac gcgcccgtgc cggtcgaggc
      481 ggacaggggc gcgcccatct cggccccggt aacgccgggg ttgatcgccg ggcggacgac
      541 cgcccccatg gccccaacgt cctcttcgcc acagttgtcg tcgcccggcc cgacggccga
      601 gcagccgaca tcgccgccga cgcagccgcc cgcgccgttg gccatccaca tggctcgtga
      661 caagttcacc gccgaacccg gccagcgtgt tgaggtcaag gtcgatgtgg aaaaccgcgg
      721 cgaccaggaa gaccgcgtga cgctcagcgt gcagggcatc gaccccagct gggtcgatgt
      781 gccggaagaa ttcaccgtcg tgccaccgcg gcaaacggtc tcgatcccct tcgccattca
      841 tccgccgcgc cgccggggta cgccggccgg ccggcagcgg ctgcgcatcg atctcaagtc
      901 gcagcgctat cccgacgccc gcgtgggcgc gacggcagcg ctgactgtcg gcccgtttgt
      961 ggctttcgaa gccagtatgc atcccactga ggtgcggctg ccggggcgaa cagtggtgac
     1021 tatccacaac actggcaacg cgccggcggc gttcaacgtc aacggcagcg acttacagca
     1081 actcgttcgc ttccgcggcg cgcgcgaggg cattgccctc cagcccaatc agacggccaa
     1141 cgtcgagttg gtgctggaga tgcgcgagac aaacctcttt ggggaggagg atgcttaccc
     1201 gtttgaggta gaggtaagca ccgggccgac agttaagcag gtcttgcccg gcgaggccat
     1261 tgcccggtcg atcatcccga caccgtggct ctatgtgctg ctgtttctaa tcattttcgg
     1321 cggcgtgttg ggcgggctgg cgctggtgtc caattggggc cgcttcctgg gcatcggcga
     1381 cgtaccaacg tccacgccga cgatcctgcc ggtgcagaac gacgccacac agaccgcggc
     1441 cgccgctgtg ctgaccgtcg ccctgggcac acagagcgcc gcggcgacca atgtggccgc
     1501 tacggcgcag gtggcgggcg ataccgacca ggatgggctg accaataacc aggagacggc
     1561 gctggggttg gacgccaaca atcctgactc agacgccgac ggcctgaaag acggcgacga
     1621 ggtattgatc tacacgacca atcccaaggt gcgcgacagc gacaacgact tgttgaccga
     1681 cttcgatgag gtcaacacct acaagaccaa tccaaacgcc aacgacagtg acggcgacgg
     1741 catccctgat gggttggagg tggggcaggg cacgaacccg ctcgtgcccg aagcgccgac
     1801 ggttacgccc atcacgccca gcccaacaac gccgccgccc tcggccacgt ggacgcccat
     1861 cgcctcgatc acgcccacgt ggacgcctat cccgtcggcc acggccacct ggacgcccat
     1921 cccctcggcc acggcgacat ggacgcctat cccctcgctg acaccgacag ccacgagcac
     1981 gctgccaccg ctgccagcgc tggcctgcgt gccggggccg atcaacattg atggcgtgct
     2041 caacgtaggc acggagtggc cggctgcgcc gactttcacc ttccagccga cggttgccgg
     2101 cgcgacgcgg ctggttaacg tctatgctgt gcgcgacgcg gggcggctct acctgggctt
     2161 cctcgtcaat gaccccgcca atgagttgac ggactccgtg cggctgtaca ttgataccac
     2221 caacaacggc ggcgacggcg acacgtctga ccgcttcttc caggtggccc gcgacggttc
     2281 gcggctgctg tgggcgggca tcggcagcaa cgccgacggc caggagtgga actccaacta
     2341 taccagcaac aactggacgg cggctgtggg cgagccgggc aacggccagt gggtcgtcga
     2401 gattcagatc gacgccgtgg ccgagctggg cgcgctgggc gatcccttcg ggctgatggc
     2461 gcaggtgctc tacaccggcg atctggccag ctacccctcg accgcctcct ccagccaggc
     2521 caacacctgg caggacatcg acaacgtgct ctgcccctag tggcgtatgt gcttgcccta
     2581 ccgccccgac ccagggctat tgccaggtca ataggagctc acgcaaagga cgctaaggaa
     2641 gcaaagagcg ccaagaaaga aagggcttgg ttcttctttg cgtccttggc gtgagcgctt
     2701 cttccgactt tgcagtgccg tgcgccccgg gcgcgggcag gctttttcat gctagaatag
     2761 agagtttatg cagcaagttg ccattacgga tttaagtccg cgcgcccgcg cgcggggctt
     2821 acgcactgtc ctggcggtgt ggttcctcat gaacgcgggc ttcttcctga tcatcccgct
     2881 cctgtcagtt cattacgtcg atcggctggg ctgggcggcc gccttcatcg gcctggtgct
     2941 ggccgtgcgc cagtttgtgc agcagggcct gaccgttttc ggtggggcgc tggccgaccg
     3001 cgtggggccg cgccggctaa tcctgctggg cgtgctcatc cgcgccgcca gtttcatcgt
     3061 catgggcttt gccagtgtgc cctggctgct gttgctgtcc gggctgctgg ccgcgttggg
     3121 tggggcgctg ttcgacgcgc ccattcgggc aacgctggcc accctggccc cggaggagga
     3181 actgacggcc atctacggcc gcctgggcat cctgcagaac gtcgcccgca cggttggccc
     3241 gctcatcggc gcttacctca tccgcttcga cttccagatg gtcggcctgg cctcagcggc
     3301 gttcttcctg ctggcctttg tcgtcactct gctcttcctg ccgcccgtca gcgtctgctc
     3361 ggagccgcag acggtgcgtg ccgggctgcg actggccctg ggcgaccgcg cctttgtcgt
     3421 cttcacgctg ctgatgatgg gcttctggtt catgtgggtg caactgtcca tcgccatgcc
     3481 actccagatc cgcgccctga ccgggcagga cagcagcgtc ggcatcatgt tcaccatcag
     3541 cgccgtgttg gccatcgtct tgcaggcccc ggcgctgcgt gtgacaaacc gctacctgcg
     3601 ctcgacgacg accattgtgc tcggcgtcgc cgtcatggcc ctggggatgg cgctgattgg
     3661 gttggcaccg ggcctggcgg cgttctatgg cgggctgttt ttcttctcgc tggggacggt
     3721 gctggccatg ccgaactcgc agacggtgac ggccgacatg tccgacgacc gcgctcgcgg
     3781 ggcctacttc ggcgtcagct cgctggcgat ggccatcggc ggcggtctgg gccacatcct
     3841 cggcggcacg ctggtggaca tggccacaac acggggcgcg ccggcgctgc cctggctggt
     3901 gttcgccggc gtgggcggag cgacggtgat tggtctggcg ctgttctact ggcgacagac
     3961 gccgccggcg gcgttccggc tcatccagcg ccccggatga ggcggcgggg cagggccggc
     4021 aggcccagcg atcgccgcag ggcgtcggcg tgttcgccat cgtgcccggc catatccagc
     4081 aagtagccgc aggcggtcgt tggcgccccc cagggggcag tgaagggccg ggccagcgtc
     4141 gcgtcgtcaa gcgtcgcggc gagggccagc agcgcccgcc gcgtggccac gaattgcgcc
     4201 cacgcttcgg ctaaggtcac gcccgtccag aacgggccgc gctcgacgtt ataggcctcg
     4261 atatcgaagg tctgctcgtc gtcaggagtc tggcccgcgg ccagtcgctg tagcgccaga
     4321 acgcccagcc ggtcgtagtc gctgatatga cccagcactt gcggcagtga ccactcgccc
     4381 tcgatggcct gcgactggcg cgcgtcgggt gggataagcg caatcagagc cagtagctcg
     4441 gcccgcgact ggcccaccag cggccgcagc aggtcacggt ggatgacccg cacggagcgg
     4501 tcgttgggcg ggtagccggc gcgccagcgg gccaggtcgg cggcgtgacc ggcgtcgtgg
     4561 cgcgcgcggc ggcgcagcca ggtcgccggc gtcgcccgcc agccgggccg cagccgcgcg
     4621 cgcccgaaga gcagcgcgtc gggcagccgg tcgagggcca gcatcaggct gcgattttcc
     4681 ttttggcaca gggccaggcc ctggctgaag ggcaggccgg cgaagcggcg ttgcagcgcg
     4741 gcgttgtggg catcaaccgt gtccgcgccg atgccgggcg gctcaatgtc gccggggcgg
     4801 ccgtcggcca gcttgcccag gcggtcggct tccagcgcgt cccaataggc caggtgggcc
     4861 agcagggcgg ccaccgtcca gccgtcgaag acggggtcgt gggtcagcgt cgcctcgtcc
     4921 aggccctcta gtcgttgtag caggtatgca cgctcggcgg ccaggcgggc cagcagcacc
     4981 gcccggtcgg gtgagagata ggcaagcatg tccatgacca ccactctacc acagatgggg
     5041 cggcgaatgg cagggctttg ccaaagtcgt ctggcgacca ttgacctgac aggtaggcgg
     5101 ccagtcgttc tgtggtggca gtcccatg
//
LOCUS       DAOSMQ010000212         5127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16397_length_5127_cov_3.459582, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000212 DAOSMQ010000000
VERSION     DAOSMQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16397_length_5127_cov_3.459582"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PK829_08225"
     CDS             complement(<1..661)
                     /locus_tag="PK829_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU41238.1"
                     /translation="MDTPTPPPIRLRPAQEAILAYRSGRMAVSAVPGSGKTFTLSLLA
                     AQLIADGRIDTDNGQQVLIVTFLNASVDTFRARIRRRLLEIGRPDTGFDVRTLHSLAL
                     EIVRWGSDLLSDDLAVLDEGQSRQFLDAAVDGWINDNARLWEAFLPDYGREYSPQMIA
                     RWRDITATTAAAFIREAKNERYRPDAIFATIRAQAEEAVDEDTPNPARSPLMHMMAGI
                     YG"
     gene            804..2447
                     /locus_tag="PK829_08230"
     CDS             804..2447
                     /locus_tag="PK829_08230"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU41239.1"
                     /translation="MSLSTRAQAEPRVDPRFGVIESFWQPEEAAELNVGWDRILFYWN
                     EIQPTGPNDWNTLHVLEEWLVEAQARDRMVVGLLKNTPAWATDGEPFSGVPRGLYLPV
                     DDPDNLWAGYVRKVVEYYAPRGVHHWIVWNEPEIDAGVYGHEFSGTPADYFQLLKVAY
                     LVAKKEDPQSVIHLAGWSYWHDPQWLDKFLRVATADPDAATNNTYFDVVTLHIYFRVE
                     TVEQLINETWDIQERYGIRKPIWVNETNASPNLDPLWPVERPSFQVDLEQQAWYVVQA
                     HALAYGAQAARVGVYKLLDILLPPGGESFGILRPDGSRRPAFDAYQTTVRYLSGFTYP
                     VGRQQTRNFYSFTFNRPEGVTRVLWARRDVAVTVHVPALADEALLVSATGEEAPLRAA
                     NGFYQVRLGGARCTPECDIGGPPVFIVETGLATRLAAVPTVNPAATLTTLTPTATPNA
                     RLTRIALSATPTNTPTETATASLTPTPTNTPTPTATPTNTPTATATASPSPIPTATAI
                     PTTPAPTTAAGASSVSSWLFLGAAVVLAGMLGLVYARRR"
     gene            2447..3433
                     /locus_tag="PK829_08235"
     CDS             2447..3433
                     /locus_tag="PK829_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU41240.1"
                     /translation="MTDVLIVGAGLAGLTAARALRQAGLRVVVVDEGGQPGGRLATRA
                     LGAGRADSGAQFFTTRSPEFTQQVAAWRAAGWVYEWSRGWSDGSLATTPPDGHPRYAA
                     RGGFRALAARLSEGLDVRLSLRVGAVAPEGDGWRVEMAGGESYAARALLLTPPAPVGL
                     GLLAAGGVVLPAGDRAALESVRFGSCLCGLFVIEGETRLPAPGALQMPAGPIGWMADN
                     QRKGISPEACVLTVHASPAASAVHWHDADDAALDWLADAARPWLAPGAVVRVAELVRW
                     PHAVPLAVLPERCRVSGLDAPLIFAGDAFGGPRVEGATLSGWAAAAALRARL"
     gene            complement(3430..4092)
                     /locus_tag="PK829_08240"
     CDS             complement(3430..4092)
                     /locus_tag="PK829_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOU41241.1"
                     /translation="MFVQRKGCLGLFIAIALLVAVPLVWRSAIKAHYRPATYTRTDSP
                     ARSVAVVFGAAVYNGRLSPVLRDRVDTAVALYLNGQVERIIMSGGQVSAQRDEPATMK
                     AYAVAQGVDAAAIITDHGGQRTYDTCYRARYVYDVRDAILVTQAFHLPRALLTCDGLG
                     VNTIGVVADQQPYRSAHWYEMRETIATVVAAWDIFRRQPPALIETAGQRSDWAMQLAG
                     DD"
     gene            4286..4921
                     /locus_tag="PK829_08245"
     CDS             4286..4921
                     /locus_tag="PK829_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU41242.1"
                     /translation="MTTAMIWGAGGGIGRALVDAVNERNWTVVAVSRDEAALRDVVAC
                     SLSADVADAFSVQRAVRAAAQEVSDIDLWLYCAGDITAAPVEDMDPAVWNRILSANLT
                     GAYLTNHYSLPLLAPNAHLMFAGAISERLRLPGLSAYGAAKAGLEAFAEALRKEQRSR
                     RVTVVRPSAVATPLWDKVPLRMPKDAAPPSKVAARILAAYDEGHTGTLDLV"
     gene            4932..>5127
                     /locus_tag="PK829_08250"
     CDS             4932..>5127
                     /locus_tag="PK829_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HOU41243.1"
                     /translation="MPEFKHSFTVAAPVEAVAAFHRDTRVLKKLTPLPILARIHRFEP
                     LADGSRAEFTLWFGPLPLRWE"
BASE COUNT          816 a         1694 c         1754 g          863 t
ORIGIN      
        1 ggccatagat gccggccatc atgtgcatca gcggcgagcg ggcggggttg ggcgtatctt
       61 cgtcaaccgc ctcctccgcc tgggcgcgga tggtagcaaa aatggcgtcg gggcggtagc
      121 gttcgttctt ggcttcgcgg ataaaggccg cggccgtcgt cgccgtgatg tcgcgccagc
      181 gggcgatcat ctgggggctg tattcgcggc cgtagtcggg cagaaacgct tcccacaggc
      241 gggcgttgtc gttgatccag ccgtccacgg cggcatcgag gaattgccgg ctctggccct
      301 cgtctaagac ggccaggtca tcactcagca ggtcgctacc ccagcggacg atctccaggg
      361 ccaggctgtg cagggtgcgc acgtcgaagc cggtgtcggg tcggcctatc tccagcagcc
      421 ggcggcggat gcgcgcccgg aaggtatcga cgctggcgtt caggaaggta acgatgagca
      481 cctgttggcc gttgtcggtg tcgatgcggc cgtcggcgat gagttgtgcc gccagcagag
      541 acagcgtgaa cgtcttgccg ctgccaggta cggcgctgac ggccatgcgc cccgagcgat
      601 aagccagaat ggcttcctgc gccgggcgca ggcggatggg tgggggggta ggtgtgtcca
      661 ttggtatcgt atctccaaga ggattataat cgccggctga ttgattcgca taaacgcaca
      721 tttgacggca tcgaagcatg aagtatgtgg cccacggacg acgactcccg gtcgtcctcg
      781 tattgtttat cctcctggcg gccttgtccc tctccacgcg ggcacaggcc gagccgcgcg
      841 ttgatccgcg ttttggcgtg atcgagtctt tctggcaacc ggaggaggcg gccgagctga
      901 acgtcggctg ggaccgcatc ctattctact ggaacgaaat ccagcccacc ggcccgaacg
      961 actggaacac cctgcacgtg ctggaggaat ggttggtcga ggcgcaggca cgggatcgta
     1021 tggtcgtggg cctgctgaag aacacgcctg cctgggcgac cgacggcgag ccgttctccg
     1081 gcgtgccgcg cggcctctat ttgcctgtcg atgaccccga taacctctgg gccggctatg
     1141 tgcgcaaggt cgtggaatac tacgcgccgc gcggcgttca tcactggatc gtctggaacg
     1201 agccggagat tgacgccggc gtctatggcc acgagttcag cggcacgccg gccgactatt
     1261 tccaactgct gaaggtcgcc tatctggtgg ccaaaaagga ggatccccag tcggttatcc
     1321 atctggccgg ctggtcctat tggcacgatc cgcagtggtt ggacaagttc ctgcgcgtgg
     1381 ccacggccga ccccgacgcc gccacgaaca acacctattt cgacgtcgtc accctccata
     1441 tctacttccg cgtggagacg gtcgagcaac tcatcaacga gacgtgggac atccaggagc
     1501 gctacggcat ccgcaagcct atctgggtca acgagaccaa cgcctcgccc aaccttgacc
     1561 cgctctggcc ggtagagcgg ccgagcttcc aggtcgatct ggagcagcag gcgtggtatg
     1621 tcgtccaggc ccacgcgctg gcctatgggg cgcaagcggc gcgcgtgggc gtctacaagc
     1681 tgctcgacat tcttttgccg cccggcggcg agtccttcgg catcttgcgg cctgatggct
     1741 cgcgccgccc cgcgttcgac gcctaccaga ccacggtgcg ctacttgagc ggcttcacct
     1801 acccggtggg gcggcagcag acgcgcaact tctacagctt taccttcaac cggccggaag
     1861 gcgtgacccg cgtactgtgg gcgcggcgcg atgttgccgt caccgtacac gtgccggctc
     1921 tggccgacga ggcgctgctg gtgtcagcga cgggcgagga agcgccgcta cgggcggcca
     1981 acggcttcta ccaggtgcgg ctcggcggcg cgcgctgtac gcccgagtgc gatattggcg
     2041 ggccgccggt cttcatcgtc gagacggggt tggcgacgcg gctggcggcc gtgccgaccg
     2101 tcaacccggc ggcgacgttg accaccttga cgccgacggc cacgcccaac gcccgtctga
     2161 cccgtattgc cctgtcggcg acacccacca acacgcccac cgagacggcg acggccagtc
     2221 tgacgcccac gcccaccaac acaccgacgc cgacggccac gcccaccaac acgccgacgg
     2281 cgacggccac ggccagccca agtcccatac ccaccgcgac cgccatcccg accacgccgg
     2341 ccccaacaac ggccgccggc gcgtcgtcgg tctcttcctg gctcttcctc ggcgcggcgg
     2401 tcgtgctggc cggcatgctg gggctggttt acgcgcgacg gcgatgatga ccgatgtcct
     2461 catcgttggc gcgggcctgg ccggtctgac ggcggcgcgg gccttgcgcc aggctggctt
     2521 gcgggtggtc gtcgttgatg agggcgggca gcccggcggc cggctggcca cccgcgccct
     2581 cggcgcaggc cgggccgaca gcggcgcgca attcttcacc acccgctcgc cagaattcac
     2641 gcagcaagtg gcggcctggc gggcggccgg ctgggtctac gagtggtcgc gcggctggtc
     2701 cgacggctcc ctggcgacga caccgcccga tggccacccg cgctatgctg cccgcggcgg
     2761 cttccgcgcc ctggcggcgc gcctgtccga aggattggac gtgcgcctgt cgctgcgtgt
     2821 tggcgcggtc gcgccagagg gtgacggctg gcgggtggag atggccggtg gcgagagcta
     2881 cgcggcgcgg gcgctgttgc tgacgccgcc ggcgccggtg ggcctgggcc tgttggcggc
     2941 cggcggcgta gttttgccgg ccggtgaccg cgccgcactg gaaagcgtcc gcttcggctc
     3001 ttgcctgtgc gggctgtttg tcattgaggg ggaaacgcgc ctgcccgcgc cgggcgcgtt
     3061 gcaaatgccg gccgggccaa tcggctggat ggccgacaac cagcgcaagg gcatctcgcc
     3121 tgaggcgtgt gttctgactg tccatgccag cccggccgcc agcgctgtcc actggcacga
     3181 cgccgacgac gcggcgttgg actggctggc ggacgcggcg cggccctggc tggctcccgg
     3241 cgcggtggtg cgggtggccg agctggtgcg ctggccccat gcggtgcccc tggccgtgtt
     3301 gccagagcgc tgccgggtga gtgggctgga cgcgccgctg atctttgccg gtgacgcgtt
     3361 cggcgggccg cgggtagagg gagcgacgct atcgggctgg gcggcagccg cggcgctgcg
     3421 ggcgcggctc tagtcgtcgc cggccagctg catggcccag tcgctacgct gcccggccgt
     3481 ctcaatgagc gccgggggct ggcggcggaa gatatcccac gcggcaacca ctgtggcgat
     3541 cgtctcgcgc atctcatacc agtgcgcgct gcggtatggc tgctggtcgg ccaccacacc
     3601 aatggtgttg acgcccaacc cgtcacaggt cagcagcgcc cgcggcagat gaaacgcctg
     3661 tgtcaccagg atggcgtcgc gcacgtcgta gacgtaacgc gcccggtagc aggtatcata
     3721 ggtgcgctga ccgccgtggt cagtgatgat cgccgcggca tcaacgccct gcgcgacggc
     3781 ataggccttc atcgttgccg gctcatctcg ctgggcggac acctggccac cgctcatgat
     3841 gatgcgctcg acctggccgt tgagatagag agcaacggcc gtatcgacgc ggtcgcgcag
     3901 cacaggactg agccgcccat tgtaaacggc cgcgccgaag acgacggcca ccgagcgggc
     3961 cgggctgtcc gtgcgcgtgt aagtggccgg ccggtagtgg gccttgatcg cactgcgcca
     4021 gacaagcggc acagccacca acagagcgat ggcgataaat agaccgagac agcctttgcg
     4081 ttggacaaac atcattgggg attataggcg caccaacaaa aggcccggac gaaaacgccc
     4141 accttctcag catcctctga cattgcgcca taacctgaca acaagcccct gaaactgcgc
     4201 gaaatattga caaaacatcg acaacgtgct aggatgagtc caatcaattt tgctcaatga
     4261 atctaatcgc tcaaacgaag acgctatgac aacagccatg atttggggcg cggggggtgg
     4321 catcggccgc gctctggtgg atgccgtgaa cgaacgcaac tggacagttg tcgccgtgag
     4381 ccgcgatgag gcggcgctgc gcgacgttgt cgcctgttcg ctgtcggccg acgtggccga
     4441 cgccttcagc gtgcagcgcg ccgtcagggc cgcggcccag gaggtaagcg acatcgatct
     4501 atggctctac tgtgccgggg acatcaccgc cgcgccggtg gaggacatgg acccggcagt
     4561 gtggaatcgc atcctgtcgg ccaacctgac cggcgcgtat ctgaccaacc actacagtct
     4621 gccgttgctg gcgcccaacg cgcacctgat gttcgccggc gcgatcagcg aacggctgcg
     4681 actgcccggc ctgtcggcct atggcgcggc taaggcaggg ctggaggcct ttgccgaggc
     4741 gctgcgcaag gagcagcggt cgcggcgcgt gacggtcgtg cggccgtcgg ccgtggccac
     4801 accgctatgg gacaaggtgc cgctgcgcat gcccaaggac gccgcgccgc cctcaaaggt
     4861 cgccgctcgc atcctggctg cctacgacga gggccatacg ggcacactcg atctggttta
     4921 gggagcacaa cgtgcccgaa ttcaagcaca gtttcaccgt cgctgcgccc gttgaggctg
     4981 tcgccgcctt ccaccgcgac acgcgcgtgc tgaagaagct gacgccgctg cccatcctgg
     5041 cccgcatcca tcgtttcgag ccgctggccg acggttcgcg ggcagagttc accctctggt
     5101 tcgggccgct gccgctgcgc tgggagg
//
LOCUS       DAOSMQ010000213         5111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16466_length_5111_cov_3.960641, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000213 DAOSMQ010000000
VERSION     DAOSMQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16466_length_5111_cov_3.960641"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK829_08255"
     CDS             complement(<1..546)
                     /locus_tag="PK829_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU41244.1"
                     /translation="MGLALTLAGATFLMAVIWGTPLIELMRRLGLGKNIRIDGPATHA
                     AKMGTPAMGGILIVAWTVLVSLVVNVVQFVQALEIVESVVIPLGVLVAYSILGGIDDY
                     QGFRLRPGEGMRARVKLAVQLVIALIAAVLLYWQVNDRHGWVAVPTVPVLLDIGLLYI
                     PAAVIVITGSANAVNLTDGLDG"
     gene            complement(546..2072)
                     /gene="murF"
                     /locus_tag="PK829_08260"
     CDS             complement(546..2072)
                     /gene="murF"
                     /locus_tag="PK829_08260"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOU41245.1"
                     /translation="MLHLGHILAALTDYQPTGNEPEVTSFVVDSREAGPGSVFVAFSG
                     ERADGHAFVADAFARGAIAALVERLPGGDWPVIDTRSRAGEPSPNPSQWAGERAAGAS
                     PGPSQRAGERMAGHLSGPSQWAGDWPAGPVLFQVDDTLRALQTVARAWRAQLDTRVVG
                     ITGSVGKTSTKELTAAVLSRRYRTLKSSGNQNNEIGVPLTLLNLRPEHERAVIEMGMY
                     AQGEIDLLCNLARPVIGVVTMIGPVHMERLGSMDAIVAAKRELVAALPPEGAAVLNYD
                     DPRVMSMAAHTRARVFTYGLDAGADLWADAIDSMGLDGVKFALHYGDETLTVGVPLLG
                     RHSVHTALRAAAVGLLEGLSWDEIIAGLSDNSAQLRLVVGHGPRGSVLIDDTYNSSPD
                     SALAALNLLADLPGRRIAVLGDMLELGRAEKQAHRVVGRRAADVADLIIAVGPRARIM
                     GGEALALGMPTDHVYIVEDAPAAVPILEETIQPGDVILIKGSLGMRMDRIVTALGRMD
                     "
     gene            complement(2169..3866)
                     /locus_tag="PK829_08265"
     CDS             complement(2169..3866)
                     /locus_tag="PK829_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU41246.1"
                     /translation="MNSSQVRRMWTVAMGMGVALGVIILRLFAFQVLHGEEWRDVVIE
                     DWAVTDTPERGVIYDRNGAVLAVDEWDYRVGISPSVLTDPEELANELAPVLQIPRGQL
                     LADLESPELYVVLAPRVPSETADAIRALAYGDEVQLDALPRRFYPQDQLMCHVLGFVD
                     FDGQGGAGVEGYYQSELAGTPASATVPLSPLRQQTTVMAREGSDLVLTIDRSVQYTVE
                     RHLKEALAEYGAVSGSIIVMDPRTGAILAMAAEPCYSANNFFDTDKALFYNPLVSAVY
                     EPGSVMKLVTMAAALDSGTVTPTTTYNDTGAITVGGHTSYNWDRGAYGTMDMTGLLAH
                     SLNVGAATIATWMGPTTFYDYFQRFGFGRRTGVDLFGEEPGLMPLPGSGEWQESFIAT
                     NAYGQALAVTPLQMISAISALANNGYQMQPYVVAEMHDENGVHVHEPTVLSQAVKPET
                     AAAVTTMAITAVQQEVQEALIDGYTVAGKTGTAQIAEPFGYNPTDTIASFVGWLPADD
                     PRVIVFVKLDRPQTAPWGSMTAAPAFSKLAKELVVLLDIPPDTVRFDAQASAAGNGG"
     gene            complement(3856..4383)
                     /locus_tag="PK829_08270"
     CDS             complement(3856..4383)
                     /locus_tag="PK829_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41247.1"
                     /translation="MTELMAVPEDKTRPKPRQARELARRLGMLTEAQAAAGWGVIVVL
                     AALLGAIYLNQASRIATIGRRVQMEQNDLDEVKRANADLERRIAEAQSLTRLDDEARR
                     LGFVPSTPADIDYVVIPQYPTEPADAAAIIPPSASLTTEPEDPPAPPETLWQAIALAF
                     RDLGIDLTRGETREQ"
     gene            complement(4451..>5111)
                     /gene="rsmH"
                     /locus_tag="PK829_08275"
     CDS             complement(4451..>5111)
                     /gene="rsmH"
                     /locus_tag="PK829_08275"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU41248.1"
                     /translation="GFVPADGILADLGLSSRQLDDAARGFSFQQDGPLDMRFDARSGA
                     TAADLINNLDAEELADIFFRYGEEQQSRRIARLIVTHRPLHTTAELATLIAREARRSG
                     RPGRHPATKVFQALRIAVNGELDEIARGLRGAVERLAPGGRLAVISFHSLEDRLVKEF
                     MRESSRDCVCPPQQPVCTCGAQPALRLVRRKAIKATDEEMAANARSRSARLRVAERTG
                     N"
BASE COUNT          840 a         1690 c         1737 g          844 t
ORIGIN      
        1 cccgtcgagg ccgtccgtca gattcacggc gttggcggac cccgtgatga cgatgacggc
       61 cgccgggatg tagagcaggc cgatgtcgag cagaacggga acggtgggca cggccaccca
      121 gccgtgccgg tcgttcacct gccagtagag caggacggcg gcgatcaggg cgatgaccaa
      181 ttgcacggcc agcttgacgc gcgcgcgcat gccttcgccc ggtcgcaggc ggaacccctg
      241 gtaatcatcg atgccaccga ggatagagta ggctaccaag acacccaggg gaatgaccac
      301 gctttcgaca atttccagcg cttgcacaaa ctgcaccacg ttgaccacca gactgaccag
      361 gaccgtccag gcgacgatta ggatacctcc catggccggc gtgcccattt tggcggcatg
      421 ggtcgccggc ccgtcgatac ggatattttt gcccagcccc agccgccgca tgagttcgat
      481 gagcggcgtg ccccagatga cggccatgag gaacgtggcc ccggccagcg tcaatgccag
      541 acccatcagt ccatcctccc cagcgccgtg acgatgcgat ccatgcgcat gcccaacgag
      601 cctttaatga ggatgacatc acccggctgg atggtctctt ccaggatggg cacggccgcc
      661 ggcgcgtcct cgacaatgta gacgtggtcg gtgggcatgc ccaacgccag cgcctctccg
      721 cccatgatgc gcgcccgcgg gccgacggca atgatcaggt cggccacgtc ggcggcgcga
      781 cggccgacga cgcggtgggc ctgcttctcg gcgcggccca gttccagcat gtcgcccagc
      841 acggcgatgc gccggcccgg taggtcggcc agcagattga gcgccgccag ggccgagtcg
      901 gggctggagt tgtaggtatc gtcgataagc accgagccgc gcgggccgtg cccgaccacg
      961 agccgtaatt gggcactgtt gtcgctcagc ccggcgatga tctcatccca gcttagccct
     1021 tccaacaggc ctacggccgc ggctcgcagc gccgtatgga cgctgtggcg gcccagcagc
     1081 ggcacgccca cagtcagcgt ctcgtcgcca tagtggaggg caaactttac gccgtccagt
     1141 cccatcgagt cgatggcgtc ggcccacagg tcggcaccag cgtcgaggcc gtaagtgaag
     1201 acgcgggcac gggtgtgcgc ggccatgctc atcacccgtg ggtcgtcgta gttcagcacg
     1261 gccgcgccct ccggcggcag ggcggcaacc agctcgcgct tggcggcgac gatggcgtcc
     1321 atagagccga gtcgctccat gtgcactggg ccgatcatcg tcaccacgcc gatcacgggt
     1381 cgggccaggt tgcagagcag gtcgatctcg ccctgggcat acatgcccat ttcgatgacg
     1441 gcgcgctcgt gctccggccg cagattcagc agcgtcaggg gcacgccgat ctcattattc
     1501 tggttgcccg acgacttaag cgttcgatag cggcgcgaca gcacggcggc ggttagctct
     1561 ttggtcgagg tcttgccgac actgccggtg atgccgacga cgcgcgtgtc caactgggcg
     1621 cgccaggcgc gggccacggt ttgcagggcg cgcagcgtgt cgtcaacctg gaagagcact
     1681 ggccctgccg gccagtcccc cgcccattgg gaggggccag agaggtgtcc cgccatccgt
     1741 tcccccgccc gctgggaggg gccaggggag gctcccgccg cccgttcccc cgcccattgg
     1801 gaggggttag gggagggttc ccccgctcgc gagcgggtgt cgatgaccgg ccagtcgccg
     1861 ccgggcaaac gttccaccag agcggcaatg gccccacggg cgaaggcgtc ggcgacgaag
     1921 gcgtggccgt cggcccgctc gccgctgaag gcgacgaaga ccgaccccgg ccctgcttcg
     1981 cggctgtcga cgacgaacga ggtcacctcc ggttcgttac cggtgggctg gtagtcggtc
     2041 aatgccgcca gaatgtgacc caggtgaagc atctcagtta ccaaatgttc gtcgttaggc
     2101 cctctcccta accctctccc ggagggcgag ggaagaggcc ctctcctgga gggcaaggga
     2161 agagaccctt agccgccatt gccggccgcc gaggcctgcg cgtcaaagcg cactgtgtcc
     2221 ggcgggatgt cgagtaaaac caccagttcc ttggccaatt tcgagaacgc cggcgcggcg
     2281 gtcatgctgc cccagggggc ggtctgcggc cggtcgagtt tcacgaaaac gatgacgcgc
     2341 gggtcgtcgg ccggcagcca gccgacaaac gaggcgatag tgtccgtcgg gttatagccg
     2401 aacggctcgg cgatttgggc tgtgcccgtc ttgccggcaa cggtgtagcc gtcgatcagc
     2461 gcttcctgaa cctcctgctg cacggcagtg atggccattg tcgtcaccgc tgcggccgtc
     2521 tccggcttga cagcctggct gagcaccgtc ggctcgtgga catggacgcc attctcgtcg
     2581 tgcatctcgg ccacgacgta gggctgcatc tggtagccat tgttggccag cgcggagatg
     2641 gccgagatca tttgcagcgg cgtcacggcc agcgcctggc cataggcatt ggtggcgatg
     2701 aacgattcct gccactcgcc gctgcccggc agcggcatca gccccggctc ctcgccgaag
     2761 aggtcaacgc ccgtgcgccg gccgaagcca aagcgctgga agtagtcata aaaggtcgtt
     2821 gggcccatcc acgtggcgat ggttgccgcg ccgacgttga gcgagtgggc cagcaagccg
     2881 gtcatgtcca tcgtgccgta agcgccccgg tcccagttat agctggtatg gccgcccacg
     2941 gtgatggccc cggtgtcgtt gtaggtggtc gtcggcgtga cggtgcccga atcgagcgcg
     3001 gcggccattg tcaccagctt catgactgag ccgggttcgt agacggcgct gaccaacggg
     3061 ttgtagaaca gggccttgtc ggtgtcgaag aaattgttag ccgaatagca cggctcggcg
     3121 gccatggcca ggatcgcgcc cgtgcgcggg tccatgacga tgatcgagcc gctgacggcg
     3181 ccatactcgg ccagggcttc cttcaggtgg cgctcgacgg tgtactgcac gctgcgatcg
     3241 atagtcagca ccaggtcact gccctcgcgg gccatgaccg tcgtctgctg gcgcaggggc
     3301 gaaaggggca cggtggcgct ggcgggcgtg ccggccagct cgctctggta gtagccctcg
     3361 acgcccgcgc cgccctggcc gtcaaaatcg acaaagccga ggacatggca catcagctgg
     3421 tcttgcgggt agaagcgacg gggcagggcg tcgagctgta cttcgtcgcc ataggccagg
     3481 gcgcgaatag cgtcggccgt ctccgagggc acgcgcggcg cgaggacgac gtagagttcg
     3541 ggcgattcca ggtcggccag cagttggccg cgggggatct gcaggacggg ggccagctcg
     3601 ttggccagtt cttccgggtc ggtcaggacg ctgggggaga tgcccacgcg gtagtcccac
     3661 tcatcgacgg ccagcaccgc gccgttgcgg tcgtagatga cgccgcgctc cggcgtgtcg
     3721 gtcacggccc aatcttcgat gacgacatcc cgccactctt cgccgtgcag cacctggaac
     3781 gcgaacaggc gcagaatgat gacgcccagg gccacaccca tgcccatggc gaccgtccac
     3841 atgcgacgca cttggctact gttcacgggt ctctcctcgc gtcaggtcga tgccgaggtc
     3901 gcgaaaggcc agggcaatgg cctgccacag cgtctctggc ggcgcgggcg ggtcttccgg
     3961 ctccgttgtg aggctggcgc tgggcgggat gatggccgcg gcgtcggccg gctcggtggg
     4021 atactgggga atgacgacgt agtcgatgtc ggccggtgtc gagggcacga agcccagccg
     4081 ccgcgcctcg tcgtcgaggc gggtcagcga ttgtgcctcg gcgatgcggc gctccagatc
     4141 ggcgttggcg cgcttgacct cgtccagatc gttctgctcc atctggacac ggcggccgat
     4201 ggtggcgatg cgactggcct gattgaggta gatggccccc agcaaggcgg ccagcacgac
     4261 gatgacgccc caaccggcgg cggcctgggc ttcggtgagc attcccaggc gacgagccag
     4321 ttcccgcgcc tggcggggct tggggcgagt tttatcttca ggcacggcca tcagctcagt
     4381 catcagtaat cagtgttcag taggacagta agccgttgtc agttgatcag tattggccac
     4441 ccggccactc ctaattccca gttctctccg ccacccgcaa ccgcgcgcta cggctgcgcg
     4501 cgttggcggc catctcctcg tccgtggcct tgatggcctt gcgacggacc agccgcagcg
     4561 ccggctgcgc cccgcaggtg cacaccggct gctggggcgg gcaaacgcag tcgcggctgc
     4621 tctcgcgcat gaactctttc accagccgat cctccagcga gtggaagctg atgaccgcca
     4681 gccggccgcc gggggccaac cgttcgacgg ccccgcgcag cccgcgggcg atctcatcca
     4741 actcgccgtt gacagcgatg cgcagcgcct ggaacacctt cgtggccggg tggcggcccg
     4801 gccggccgct gcgtcgcgcc tcgcgggcga tcagcgtcgc cagctcggcg gtggtgtgca
     4861 gcggccggtg agtgacaatg agccgggcaa tgcgccggct ctgctgctcc tcgccataac
     4921 ggaagaagat gtcggccagt tcttccgcgt ccaggttgtt aatcaaatcg gcggccgtcg
     4981 ccccactacg ggcatcgaac cgcatgtcca gcgggccgtc ctgctggaaa gaaaagcccc
     5041 gcgcggcgtc gtccagttgc cgcgacgaca gccccaggtc ggccaagatg ccatcggcag
     5101 gcacaaaacc g
//
LOCUS       DAOSMQ010000214         5110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16471_length_5110_cov_4.616419, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000214 DAOSMQ010000000
VERSION     DAOSMQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16471_length_5110_cov_4.616419"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK829_08280"
     CDS             <1..200
                     /locus_tag="PK829_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41249.1"
                     /translation="LGGCQYSVEVHRWLNYSPSARPGNQPIRADADFQLSPIDLKPDR
                     LYVAQVVQPVRLSPAAAIATA"
     gene            complement(505..4653)
                     /locus_tag="PK829_08285"
     CDS             complement(505..4653)
                     /locus_tag="PK829_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41250.1"
                     /translation="MTTTQTIVWIALPNGYAGEAPERRLRLSIFVAPRLRSDAGRTLA
                     LYPDFLDWAARLGPDVASFALEVDDGTVVPATVVSEPPDPLLWAALFHAETPVRPFQF
                     DDYADRPIVSYPVRDVLNFVKRVYRETAAASPTDLPRIAPDPDDPERGPALTQLFREL
                     IHGPHREIMRVPNEAALSERLSGSLERARQQAQARRAAGQRGGPLIEPDSIAPAGTPS
                     DSFYRTMLFHYRPAQAKPVELPASEDEAATLFTAEFDFHQMLTALGDYPALLRRLGLI
                     VDLEVPDGALPVSDNEFAQRKLRVNATWASNFAPLPADPGALNVSPWTVYALASLNGQ
                     DLFISWPTRDAGAGMWLPVAGAVDVVQVDADGAALRTLHQVNSIDRLTDGRPVAIDAP
                     ERDGLGALRTSGLAIVQTGRAAAMAEDFNNSLALNKLLDQNPPAPVDLRAEDVQRGYR
                     LDVQDVTTGLWYSLHRRVGTFNPLDDARGPFDVADEGFTQPSAATPAEKPGTPPDPNA
                     ELYIHEALFTWDGWSLSAPRPGKALSRSARAPSPDEPGTEPDYVPNKAMTALRLETSF
                     RPQDGSLPRLRYGRPYRLRVRAVDLAGNSPTVDEATALLAKLGGLFQPVLPVDREALY
                     SRFEPVNPPEIVPRAEFTEGESAARLVIRSDFDRSAETYAAEHPPYAAVNERHIVAPK
                     AALAQIEAHGLLDAAFDAKPGALTEEEVRAIRQEVYELARREAGSLTDTSLPTVIFVP
                     TSAEAPTQGYAVHTEEQLELPYLPDPWSAGAVFQGLPGTAPGQLTSIVWGGLAWPKAT
                     PFRLQLAEGDAPPQWDEATRVLTVFLPGSGAATVRISSLFGGDLTHVGLWRWLEEAVE
                     AGEMDPARLDEISVAAKEGRHWMFTPFQELTLVHATQHPLAQPEFLELSAERPYGQAI
                     AYLSGAIDLHAPSTAKIDLLATWTEPRDDPAAPGPDRVNGQAHVMEIPIDLRNQPIAV
                     PHLEAPGRFVLLLEDDRLLTFDAHGCAIVAQNIRWRQANEPPPPPHEADRREKRRLLA
                     EKIAAHDFGDTKYRRVRYHPAATSRFREYFPSDLWGELSRAGGEIELDIPASARPAAP
                     QLRYALPTFGWTAEDDGATASSRRLGGGVRLCLERSWYSSGDGELLAVVLGQGVPDPR
                     DHLYRYSTFWGQDPLWVSPPLELPRPAAFRNADLVETHVALGELPGSTVTVVGFLPRW
                     DDSRSLWTVDLDLPTAGHYFPFIRLALARFQPRALSDVRLSSVVLADFVQTAPDRLLT
                     IARDAGGPGVHRVHVSGAAYTARRGFNNVVTPATSQMTARLERRRPDIADETLGWTAV
                     AEAAAVVLTPGAVDADGTGHWEGDITVPAAAAGERLRLVVREEESYENTTAGRPVYVD
                     VVAL"
     gene            complement(4678..>5110)
                     /locus_tag="PK829_08290"
     CDS             complement(4678..>5110)
                     /locus_tag="PK829_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41251.1"
                     /translation="FFAMALSADGLEQIEAAIEFGGNLSLNLGVASGGVYVMAGIYFS
                     LAGDETKLTGYLRCGGYLEVLGIISISIEFYLGFTFRSKGGGGGEVWGQASVKVAVKI
                     AFFSVSVTLTVERRFAGAAGDPTFEQVVGPDDWNTYCAAFA"
BASE COUNT          822 a         1720 c         1810 g          758 t
ORIGIN      
        1 gcctcggcgg ttgtcagtac agcgttgagg tccataggtg gctcaactat agcccgtccg
       61 cccgccccgg caaccagccg atcagggcag acgcggattt tcagttgtca ccaattgacc
      121 tgaaacctga ccggctgtat gtggcccaag tagtacagcc tgtcaggctg tcgccggccg
      181 cagccatagc cacagcctaa caggctgtgc tacaccacag cctgaccggc tgtatgcggc
      241 ccaagtagta cagcctgtca ggctgtcgtc agccacagcc atagccacag ccacagccac
      301 agcctaacag gctgtgctac accacagcct gaccggctgt atgcggccca agtagtacag
      361 cctgtcaggc tgtcgtcggc cgcagccaca gcctaacagg ctgtgctaca ccacagcctg
      421 acaggctgtg ctacagacta ctggaagcac tgtcagggca ggcgcgccgt ctgtggcggc
      481 gtgtacatcc tgagaggcgc gcgtctataa agcgacgaca tcgacgtaga ccggccgccc
      541 ggcggtggta ttttcgtaag actcctcttc gcgcacgact agccgcagcc tctcgcccgc
      601 ggccgccgcc ggcacggtga tgtcgccttc ccagtggcca gtgccgtcgg catcgaccgc
      661 gccgggcgtc agcaccaccg cggcggcctc agccacggcc gtccagccca gcgtctcgtc
      721 ggcaatgtcc gggcggcggc gctccaaacg cgccgtcatc tggcttgtcg ccggcgtcac
      781 gacgttgttg aacccccgcc gtgccgtgta ggccgcgccg ctgacgtgga cgcggtggac
      841 gcccggcccg ccggcgtcgc gggcgatggt cagcagccgg tcgggcgcgg tctgcacgaa
      901 gtcggccaac accacgctcg acagacgcac atccgacagc gcccgcggct ggaagcgggc
      961 cagcgccagg cggatgaacg ggaagtagtg cccggccgtg ggcaagtcaa gatcgaccgt
     1021 ccacaggctg cggctgtcgt cccagcgagg cagaaacccg acgacggtca cggtgctacc
     1081 gggcaactcg cccaaagcca cgtgcgtctc caccaggtcg gcattgcgga aggcggccgg
     1141 ccgcggcagt tccagcggcg ggctgaccca cagcgggtct tgcccccaga aggtggagta
     1201 gcggtagaga tggtcgcgcg ggtcgggcac gccctgcccc aggacgacag ccagcaattc
     1261 gccatcgccg gaggagtacc acgaccgttc caggcagagg cgcacgccgc cacccagtcg
     1321 ccggctgctg gccgtcgcgc cgtcgtcctc ggccgtccag ccaaaggtag gcagggcata
     1381 gcgcaactgg ggcgcggccg ggcgcgccga ggcggggatg tccaactcga tctcgccgcc
     1441 ggcacggctc aactcacccc acaggtcaga cggaaagtac tcgcgaaagc gggaggtggc
     1501 cgccgggtga tagcggacac ggcggtactt ggtatcacca aagtcgtggg cggcgatctt
     1561 ctccgccagc agccggcgct tctcgcgccg gtcggcctcg tgcggcggcg ggggcggctc
     1621 gttggcctgc cgccagcgga tgttctgggc gacaatggcg cagccgtggg cgtcgaaggt
     1681 tagcaaccgg tcatcctcca gcagcaggac aaagcggccg ggcgcttcca ggtgcggcac
     1741 ggcaatgggc tggttgcgca ggtcgatggg aatctccatg acatgggcct ggccgttcac
     1801 tctgtccggg ccgggcgcgg ccgggtcgtc gcgcggctcc gtccacgtcg ccaacaggtc
     1861 gatcttggcc gtggagggcg cgtgcaggtc gatggcccca ctcaagtagg cgatggcctg
     1921 gccatagggg cgctcggccg acagctccag aaactccggc tgggccagcg ggtgctgcgt
     1981 ggcgtgcacc aaggtcagtt cctggaaggg agtgaacatc cagtggcggc cttctttggc
     2041 cgcgacggag atctcgtcca gacgcgccgg gtccatctcg cccgcctcga ccgcctcctc
     2101 cagccagcgc cacaggccga catgggtcag gtcgccgcca aagagagagc tgatgcgcac
     2161 cgtggccgcg ccggagccag gcaggaagac ggtcagcacg cgcgtggcct cgtcccattg
     2221 tggcggggcg tccccctcag ccagttgcag ccggaacggc gtcgccttgg gccaggccag
     2281 cccaccccag acgatgctcg tcaactgccc cggcgctgtg cccggcagcc cctggaagac
     2341 ggctccggcc gaccatggat cgggcaggta gggcagttcc aactgctcct cggtgtggac
     2401 ggcgtagccc tgcgtcgggg cttcggcgct ggtgggcacg aagatgaccg tcggcaggct
     2461 tgtgtcggtc agcgagccgg cctcgcgccg cgccagttcg tagacctcct ggcggatggc
     2521 gcgcacctcc tcctctgtca gcgcgcctgg cttggcgtcg aaggcagcgt cgagcaggcc
     2581 gtgggcctcg atctgggcca gagccgcctt gggcgcgacg atgtggcgct cgttgacggc
     2641 ggcatagggc gggtgctcgg cggcgtaggt ctcggccgag cggtcgaagt cagagcgaat
     2701 gaccagccgc gccgccgact cgccctcggt gaactcagcc cgcggcacaa tctccggcgg
     2761 gttaaccggc tcgaaacggc tgtagagcgc ctcccggtcg acgggcaaca ccggctggaa
     2821 cagccccccc agcttagcga gcaacgccgt ggcttcgtcc actgtcgggc tgttgccggc
     2881 caggtcgacg gcacgcacgc gcaggcggta gggacggcca tagcgcagcc gcggcagcga
     2941 gccgtcctgc ggccggaagc tcgtctccag ccgcagcgcg gtcatggcct tgttaggcac
     3001 gtaatccggt tccgtgcccg gctcgtcggg cgacggggcg cgggcgctgc ggctgagggc
     3061 cttgcccggc cgcggcgcgg acaggctcca gccgtcccag gtgaacagcg cttcatgaat
     3121 gtagagttcg gcgttggggt cgggcggcgt gccgggcttc tctgccggtg tcgccgcgga
     3181 tggctgggtg aagccctcat cggccacatc gaaggggccg cgggcgtcgt cgaggggatt
     3241 gaacgtgccg acgcggcgat ggagcgagta ccacaggccg gtggtcacgt cctgcacgtc
     3301 cagccgatag ccgcgctgca cgtcctcggc gcgcaggtca accggcgcgg ggggattctg
     3361 gtctagcagc ttgttgaggg ccagggagtt gttgaagtcc tcggccatgg ccgcggcgcg
     3421 gccggtttgg acgatggcca ggccgctggt gcgcagcgcg cccaggccgt cgcgctccgg
     3481 ggcgtcaatg gccacaggac ggccgtcggt cagacggtcg atgctgttga cctggtggag
     3541 tgttcgcagg gccgcgccgt cggcgtccac ctggacgaca tccaccgccc cggcgacggg
     3601 cagccacatg cccgcgcccg cgtcacgtgt cggccaggag atgaacaggt cttgcccgtt
     3661 cagcgacgcc agggcgtaga ccgtccaggg gctgacgttg agcgccccgg ggtcggctgg
     3721 cagcggcgcg aagttggaag cccacgtggc gttgacgcgc agcttgcgtt gggcgaactc
     3781 gttgtcggaa acgggcagcg cgccgtcggg cacttccagg tcaacgatca ggcccagccg
     3841 ccgcagcagc gccgggtagt ctcccagggc agtcagcatc tggtgaaagt cgaactcggc
     3901 cgtgaagagc gtcgcggctt cgtcttcgct ggccggcagc tcgacaggct tggcctgggc
     3961 gggccggtag tggaacagca tcgtccggta gaagctatcg ctgggcgtgc ctgccggggc
     4021 gatgctgtcc ggctcgatga gcggaccgcc gcgctggccg gccgcccgcc gcgcctgggc
     4081 ctgttgccgc gcccgctcca gcgagccgga caggcgctca ctgagcgctg cctcgttggg
     4141 cacgcgcatg atctcccggt gggggccgtg gatcagctcg cgaaacagct gggtcagcgc
     4201 cggcccgcgc tccgggtcgt ccgggtcggg ggcgatgcgc ggcaagtcag tgggcgaggc
     4261 ggcggccgtc tcgcggtaga cgcgcttgac gaagttgagc acgtcgcgca cggggtagct
     4321 gacaatgggc cggtcggcgt agtcgtcgaa ttggaacggc cgcaccggcg tctctgcgtg
     4381 gaagagcgcc gcccacagca gcggatcggg cggctcactg accacggtgg ccggcacaac
     4441 cgtgccatca tcgacttcta gcgcgaacga agccacgtcc gggccgaggc gcgccgccca
     4501 gtcaaggaag tcgggataga gcgccaatgt gcggccggcg tcgctgcgca ggcgcggggc
     4561 aacgaaaatc gacagccgca gccgccgctc cggcgcttcg ccggcgtagc cattaggcag
     4621 ggcaatccac acaatcgtct gagttgtggt cataggacat cctgatgggc cgggcgctta
     4681 ggcgaaagcc gcgcaatagg tgttccagtc gtcggggccg accacctgct cgaaagtcgg
     4741 gtcgccggcc gcgccggcga accgccgctc aacggtgagg gtcacgctga cgctgaagaa
     4801 ggcgatctta accgccacct tgaccgaggc ctggccccag acctcgccac cgccaccccc
     4861 cttgctacgg aaggtgaagc ccagatagaa ctcgatggag atggagatga tgcccagcac
     4921 ttccagatag ccgccacagc gcaggtagcc ggtcagcttg gtctcgtcgc cggccaggct
     4981 gaagtagatg ccggccatga catagacgcc gccgctggcc acgccgaggt tgagcgacag
     5041 attgccgcca aactcaatgg ccgcctcgat ctgctccagg ccgtcggcgc tgagggccat
     5101 ggcaaaaaag
//
LOCUS       DAOSMQ010000215         5099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16524_length_5099_cov_4.595361, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000215 DAOSMQ010000000
VERSION     DAOSMQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16524_length_5099_cov_4.595361"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="PK829_08295"
     CDS             <1..1468
                     /locus_tag="PK829_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU41252.1"
                     /translation="TFRQMNARANRLANWLRDVAGVGRGDRVAILAHDGVEHLDTFFA
                     CGKLGALHTALNWRLHPQELAGIIAQTTPKVLIFSATVAATGDMASKVAALQDLLRGR
                     PEAIGYSLHTEGDAVGGSLPFEATLQAAPATPVTTDELEAEDIAALIFTGGTTGLPKA
                     AQVSHRMIAWNTLNTVIHDVTHNDIYLNVFPMFHTGGLFVYTLPQIIFGGTTILLRQF
                     DARRVLELIERERVTVFGAVPTMYQVITQASNWATADLSSLRFCTSGGAPLPVPLVEQ
                     YTREKGIRFKQGFGMTEFGPGIFALAPEDAIRKAGSIGRPNFYVDARIVDDDDNFLGP
                     DQDGELVLKGPSYCSGYFGDPEASAAAVDDRGYFHTGDMARYDSEGYFTIVDRKKDMF
                     ISGGENIYPVEIEKVLYQHPAVLMCAVIGLPDPKWGEVGRAYVVLRPGTQASEAELLA
                     FTAARLARYKVPKSVVLRPELPISAAGKILKRELRDEY"
     gene            1562..2617
                     /locus_tag="PK829_08300"
     CDS             1562..2617
                     /locus_tag="PK829_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU41253.1"
                     /translation="MSSIPTLPGITSAFVPTARLLTHVLTSGPADGEPVVFVHGNASS
                     SVFWEETMLALPPRFRAIAPDLRGYGETEDLLIDATRGAKDWAEDLKALSDTLGERPA
                     HLIGWSLGAAPVLQFALDYPALVRSITLVAPVSPYGFGGSKDNDGRSNYDDFAGSGGG
                     SVNPEFVRRMQIGDRGSDDANAPRMVINTFYYKPPFRAAREEEFLSSLLLEKTGADRY
                     PGDMLPSTNWPTVAPGVWGPINATSPKYLNLSSITDLAQKPPILWVRGDSDQVVSDNS
                     LFDFGTLGMLGAVPGWPGAEVFPPQPMVSQTRAVLDRYAANGGRYQEVVITDVGHSPH
                     IEQPEAFMVALLSHLEG"
     gene            2631..4166
                     /locus_tag="PK829_08305"
     CDS             2631..4166
                     /locus_tag="PK829_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU41254.1"
                     /translation="MYTFDWLAKQADLRPDKTALIDAATGRRLTYPQFHLRAGRFAEF
                     LRREWRLEPGERVALLAPNSSDYFEILWGCAKAEVILVCLNWRLAVPELEFILRDATP
                     SALVYDPAFAEAALTLAGRLGIDRLMALSASRDSAPPEGVAAYEDALASASGREVVMR
                     PRRLDETWHLLYTAGTTGQPKGVLQTFGMVFYNALNLGLAIGLTDEDVTLNLLPCFHT
                     GGLNMYTNPTFHVGGTAIVQKAFDPAETLRLLSEEATAIFGVSAVYLFLSQHPDFERT
                     TFRQMRSWAAGGAPIPTSLLERYLDRGIEIQFGFGMTETGPTVFLIEKGAGRAKLGSV
                     GKPQLYVDVRIVDREGRDLPPGERGELLIKGPGVTPGYWQRPDITAASIVDGWLHSGD
                     VAVRDADGYYTIVDRWKDMFISGGENVYPAEVENVIYQLPAVAEAAVVGVPDARWGEV
                     GRAFVVLRPGETLDAAAVIAHCRNQIAAYKAPKSVVFIDKLPRNAAGKVVKDELRRLA
                     QPA"
     gene            4184..5056
                     /locus_tag="PK829_08310"
     CDS             4184..5056
                     /locus_tag="PK829_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOU41255.1"
                     /translation="MRLTIGDVITEPMTFTQAQFDRFAALSGDDNPIHVDPTFAARSR
                     FGRTVAHGMFLYAHLRRALGELLPGVGQREGALMFTAPTVADEPVQLRLEIVDVDAAG
                     RVWVNTAVARSDGQFGCRGDAIMQIGGGDDETTFQQRPLSPTERAGERDLRGLSVGQS
                     ASLRRAYTPAEHDAYLALLGETNPLFSDAAYAQRRGLRGLPLPGPLLGALFSCIMGTR
                     LPGRGTNWLKQRFHFCQYAFPGDDLEAHVQIVRLRPDKELANLRTWITSAGGLVCDGE
                     ALVWVGDLVNQARN"
BASE COUNT          821 a         1752 c         1642 g          884 t
ORIGIN      
        1 gacgttccgc cagatgaacg cgcgcgccaa ccggctggcc aactggctgc gcgacgtggc
       61 cggcgtcggc cgtggcgacc gtgtggccat tctggcccac gacggcgtgg agcatctcga
      121 cacctttttc gcctgtggca agctgggcgc gctgcacacg gcgctgaact ggcggttgca
      181 cccgcaggag ttagccggca tcatcgccca gacgacgcca aaggtcctca tcttttcggc
      241 caccgtggcg gccacgggcg acatggccag caaggtggcc gcgttgcaag acctgttgcg
      301 cggccggcca gaggctatcg gctatagcct ccacacagag ggcgatgctg tcggcggcag
      361 cctgcccttt gaggccacac tccaagccgc cccggccacg ccggtgacca ccgacgaact
      421 ggaggcggag gacattgccg cgctgatctt caccggcggc acgaccggcc tgcccaaggc
      481 ggcccaggtg tcccatcgca tgatcgcctg gaacacgctc aacaccgtca tccacgacgt
      541 gacccacaac gacatctatc taaacgtctt ccccatgttc cacaccggcg gcctgttcgt
      601 ctacaccctg ccgcaaatca tcttcggcgg cacgaccatc ctgctgcgcc agtttgacgc
      661 ccggcgcgtg ctggaactga tcgagcgcga gcgcgtgacc gtcttcgggg ctgtgccgac
      721 gatgtaccag gtcatcaccc aggcgtccaa ctgggctacg gccgacctct cctcactgcg
      781 cttctgcacc tccggcggcg cgccgctacc cgtgccgctg gtggaacaat acacccgcga
      841 gaagggcatc cgcttcaaac agggctttgg catgaccgag ttcgggccgg gcatcttcgc
      901 tctggctccg gaggacgcca tccgcaaggc gggcagcatc ggccggccca acttctacgt
      961 cgatgcccgc attgtcgatg acgacgacaa cttcctcggc cccgatcagg acggcgaact
     1021 ggtgctgaag gggccatcgt actgctccgg ctacttcggc gatccggagg ccagcgccgc
     1081 cgccgtcgat gaccgcggct acttccacac cggcgacatg gcccgctatg acagcgaggg
     1141 ctacttcacc atcgtcgacc gcaagaaaga catgtttatc agcggcggcg agaatattta
     1201 tcccgtcgag atcgagaaag ttctctacca gcatccggcc gtgctgatgt gcgctgtcat
     1261 cggcttgccc gaccccaaat ggggtgaggt cggtcgcgcc tacgtggtgc taaggccggg
     1321 cacgcaggcc agcgaggccg agctgttggc gttcacggcc gcccgcctgg cccgctacaa
     1381 ggttcccaaa agcgtcgtcc tgcggccgga gttgcccatc tcggccgcgg gcaaaatcct
     1441 gaaacgtgag ttgcgcgacg agtattgaca gggataagga ctggtttgtc ggggcgggtc
     1501 ttagacttgc ccctacaccc gtcccgacac ccacccacta gaccgttaca aggagcatcc
     1561 catgtcatcc atccccaccc tgcctggaat cacctcggcg tttgtcccca cggcccgcct
     1621 gctcacccac gtactcacca gcggcccggc cgatggcgag ccggtggtct tcgtccacgg
     1681 caacgcttcg tcgtcggtct tctgggaaga gacgatgctg gccctgccgc cgcgttttcg
     1741 ggccatcgcc cccgacctgc gcggctatgg cgagaccgaa gacctgctca tcgacgccac
     1801 ccgcggggcg aaggattggg ccgaagacct gaaggccctg tccgacacgc tgggcgagcg
     1861 gccggcccac ctgatcggct ggtcgctggg ggccgcgccg gtgctgcaat tcgctctgga
     1921 ctacccggcg cttgtccgca gcatcaccct cgtcgccccg gtcagtccct atggcttcgg
     1981 cggctcaaag gacaacgacg gccggtccaa ctacgacgac ttcgccgggt cgggcggcgg
     2041 ctcggtcaac ccggaattcg tccgccgcat gcagatcggc gaccgtggca gcgacgacgc
     2101 caacgcgccg cgcatggtca tcaatacttt ctactacaag ccgcccttcc gcgccgcccg
     2161 cgaggaagag ttccttagct cgctgctgct ggagaagacg ggcgcggacc gctaccccgg
     2221 cgacatgctg ccctcgacca actggccgac cgtcgctccc ggcgtctggg ggccgattaa
     2281 tgccacgtcg ccgaagtacc tgaacctgag cagtatcacc gacctggccc agaagccgcc
     2341 cattctgtgg gttcgcggcg acagcgacca ggtcgtcagc gacaattcgc tgttcgactt
     2401 cggcacgctg ggaatgctgg gagccgtgcc cggctggccg ggggcggagg tcttcccgcc
     2461 acagccgatg gtgtcccaga cgcgcgctgt cctcgaccgc tacgcggcca atggcggccg
     2521 ctaccaggaa gtcgtcatca ccgacgtcgg ccattcgccc cacatcgagc agccagaggc
     2581 cttcatggtc gcactgctga gccatctgga gggctagagc ggagggcgct atgtacacct
     2641 tcgactggtt ggccaagcaa gcggatctac gccccgacaa gacggcgctg atcgacgcgg
     2701 ccacggggcg gcgactgacc tacccccagt ttcacctgcg cgccggccgc tttgctgagt
     2761 tcctgcggcg cgagtggcgg ctggagccgg gcgagcgcgt ggcccttctg gcccctaact
     2821 cgtccgacta cttcgagatc ctctggggct gcgccaaggc cgaggtcatc ctggtctgcc
     2881 tcaactggcg gctggccgtg ccggagttgg agttcatcct gcgcgatgcc acgccgtcgg
     2941 ccctggtgta cgaccccgcc tttgctgagg cggcgctcac gctggccggg cgtttgggca
     3001 tcgaccggct gatggccctc tcggccagcc gcgatagcgc gccgccggag ggcgtcgctg
     3061 cttacgagga cgcgctggcg tccgcctctg gccgcgaggt ggtgatgcgt ccccgtaggc
     3121 tggacgagac gtggcatctg ctctacaccg cgggcacgac cggccagccc aagggcgttt
     3181 tgcagacctt cggcatggtc ttttacaacg ccctgaatct gggcctggcc atcggcctga
     3241 ccgacgagga cgtgacgctc aacctgctgc cgtgcttcca caccggcggg ctgaacatgt
     3301 acaccaaccc gacctttcac gttggcggca cggctatcgt ccagaaagcg ttcgatccgg
     3361 ccgagacgct gcgcctgctg tcggaggagg ccacggccat ctttggcgta tcggccgtct
     3421 acctgttcct tagccagcac cccgacttcg agcgcacgac cttccgccag atgcgttcct
     3481 gggccgccgg cggcgcgccc atccccacca gcctgctgga acgctatctc gaccgtggca
     3541 tcgagattca gttcggcttt ggcatgaccg agacggggcc gaccgtcttc ctgatcgaga
     3601 agggggctgg ccgcgccaag ctcggctccg tgggcaagcc gcagctttac gtcgatgtgc
     3661 gcatcgtcga ccgcgagggg cgcgacctgc cgcctggcga gcgcggcgaa ctactcatca
     3721 aggggccggg cgtcaccccc ggctactggc agcggcccga cattacggct gcgtccatcg
     3781 tggacggctg gctccactct ggcgacgtgg ccgtgcgcga tgccgatggt tactacacca
     3841 tcgttgaccg ctggaaggat atgttcatca gcggcggcga aaacgtctac ccggctgagg
     3901 tcgagaacgt catttaccag ttgccggccg tggccgaggc cgctgtcgtc ggcgtgcccg
     3961 acgcgcgttg gggcgaggtg gggcgggcct tcgtcgttct ccggccgggc gagacgctcg
     4021 atgccgcggc ggtcatcgcc cactgccgca accagatcgc cgcctataaa gcgcccaagt
     4081 cggtcgtctt cattgataag ctgccgcgca acgctgccgg caaggtcgtt aaggacgagt
     4141 tgcgccggct ggcgcagccg gcctaggcga tgaggatacg gccatgagat tgactatcgg
     4201 cgatgtcatc acggagccaa tgaccttcac ccaggcgcag ttcgatcgct tcgcggcgct
     4261 cagcggtgac gacaacccca tccacgtcga tcccaccttt gccgcgcgca gccgcttcgg
     4321 ccggacggtg gctcatggca tgtttctcta cgcccacctg cggcgggcgc tgggagagtt
     4381 gctgccgggc gtgggccagc gcgagggcgc gctgatgttc accgcgccga cggttgccga
     4441 tgagccggtg cagctgcggc tggagatcgt cgatgtggac gcggctggcc gcgtttgggt
     4501 caataccgcc gtggccagga gcgacggcca gtttggctgc cgcggcgacg cgatcatgca
     4561 gatcggcggc ggcgatgacg agaccacttt tcagcaacgt cccctctctc ccacggagag
     4621 ggctggggag agggatctgc gcggccttag cgttggccag tcggcgtcgc tgcggcgcgc
     4681 ctacacccct gccgaacacg atgcctacct ggctctgctg ggcgagacca atccgctctt
     4741 cagcgacgcg gcctacgccc aacggcgcgg gctgcgcggt ttgcctttgc cggggccgct
     4801 gctgggggcg ctgttctcct gcatcatggg cacgcgcttg cccggccggg gcacaaactg
     4861 gctcaagcag cgcttccact tctgccagta cgcttttccc ggtgacgacc tggaagccca
     4921 cgtgcagatc gtccgcttgc ggccagacaa ggaactggcc aacctgcgga cgtggatcac
     4981 gtcggctggc gggctcgtct gcgacggcga ggcgctggtg tgggttggcg atctggtcaa
     5041 tcaggcgcgg aactagggca tacaggtgag tgttgctatg tcaacggtca acgtcaacg
//
LOCUS       DAOSMQ010000216         5089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16562_length_5089_cov_5.294199, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000216 DAOSMQ010000000
VERSION     DAOSMQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16562_length_5089_cov_5.294199"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK829_08315"
     CDS             <1..262
                     /locus_tag="PK829_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018468313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41256.1"
                     /translation="SFRLRGHQFTDADGRYTLETILPGPIPGRAPRLYVRVQPPGGPV
                     LTTQLFFPDDPANASDAEFQPALVVTPAGEGIASFDFVVATQ"
     gene            complement(278..1207)
                     /locus_tag="PK829_08320"
     CDS             complement(278..1207)
                     /locus_tag="PK829_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU41257.1"
                     /translation="MILNRRRRRALLFLLLIFGPLTLLASVVMVYALGGPAVGERAHR
                     LSAFGGLRLLLPQTVNYTDSDGPLYRGQHATSAARPPDELLAVSYNLHFGDALDAARR
                     AFEARDPLPAPDFLLLQEVDAAGTESLAAALNYDYVYFPASVAPDGDDFGNAILSRWP
                     LSDAHKLILPGVHPLSGQQRTATRATARVGGREVLLYSTHIEIATAPVALRAAQMAAI
                     ATDVPADATAVLIGGDFNVVTGRGARALARLYADHDLDHASQTAGPTLTRLGVRAPAD
                     HLFTRGFEVVAAGAAGDMTASDHLPVWAWLRWR"
     gene            complement(1366..2358)
                     /locus_tag="PK829_08325"
     CDS             complement(1366..2358)
                     /locus_tag="PK829_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOU41258.1"
                     /translation="MISIYRDGENGLLVADGFPPGAWIHAESPSEVELALLQRELGLP
                     PSFLAAAVDPREVARTDTQRGMHLTVVRVPYNFGARERVPFRTTPLGMIIAPNHFLTI
                     CRRPIDFVHDLTAIYSATTLPTRRANRMILAILGIVGQWFLRDLEEIDRLMDDVEDRL
                     INSIENGEMMELLRYEKSLVYIKTGLEWNETMVRRLQDGSHFGWTDDDLEMLREVEIE
                     YRQGAHMAATMQAVSGEMTDTFASLISNNLNVVMKFLAAVTIILTVPMLFTSFYGMNV
                     ALPGRDTRPAFVVMTLLSIGVTVLVSWWFWRQGWLSFRWRVGSDARDGGDDPGV"
     gene            2682..3755
                     /locus_tag="PK829_08330"
     CDS             2682..3755
                     /locus_tag="PK829_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41259.1"
                     /translation="MPAKQNRHASSWWFFLLCPEGPDSYGPRQLMFTVAARAGQRIRI
                     NDVWLPGLDRARPVTGGVDRFDAMSVGWYCDGEAVHDLLRLAGRAEMDATAGSLRWGD
                     GHGHGVTFRQSAARPVGLAATVCGPGGAAEFEAWSTLDSAYDAPTVSMHIDTPFGGTH
                     YIGWRRLNFRGRFELPTGVETLQGTGFFQRVCLNVPVFPWKWIWTIFPDRSVFTAYLP
                     YVGLNLFRKGYRFFRSERLEQAALSIKPSAMWVPAGGTPIRFDRVSALPRLGRGPYPQ
                     FDVRATNAAGDAIAFTAAPYGLSRFYIDRPVLGGLVETHWNYNEFMFRMDDLAGSIAG
                     RSLSRATMGQAYGSFEYAYGLGL"
     gene            3820..4956
                     /gene="meaB"
                     /locus_tag="PK829_08335"
     CDS             3820..4956
                     /gene="meaB"
                     /locus_tag="PK829_08335"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU41260.1"
                     /translation="MPSSPRKPDWTPDDAGDAFTARIISGVAGRHDGLDAGRDAATPP
                     VAATRGPARRPLSIDDYVAGVLSGDRAVLARAITLIESNAPTHFETAQEVLRRLLPHS
                     GNALRVGITGVPGAGKSTLIEALGLALIAAGHRVAVLAIDPSSSLTRGSILGDKTRME
                     GLSRVAEAFIRPSATGGVLGGVARKTRETMLVCEAAGFDVILIETVGAGQNEVTVRSM
                     VDFFLLVLIPGGGDELQGIKKGVVELADAIAINKADGDNRVRAEAARAEYNRALHYLT
                     PATDSWHARAYTCSAETGEGVANLWAVVERFRERTTESGAFAARRRAQAREWLHTLLD
                     EQLRAAFYDRPAVRAALPPMEAAVMAGELSATAAARQLLALLDG"
BASE COUNT          839 a         1794 c         1656 g          800 t
ORIGIN      
        1 cagcttccgc ctgcgcggcc accagttcac cgacgccgac ggccgctaca cgctggagac
       61 gatcctgccc ggccccatcc ccggccgcgc gccgcgcctc tacgtcaggg tgcagccccc
      121 cggcggcccc gtgctgacga cgcagctctt cttccccgat gaccccgcca acgccagcga
      181 cgccgagttt cagccggcgc tggtggtcac gccggccggc gagggcatag cgagcttcga
      241 tttcgtggtg gcgacgcagt aggctgacgc gcctcgccta gcgccaccgc aaccacgccc
      301 acaccggcaa atgatcgctg gcggtcatgt cgccggccgc gcccgcggcc accacctcaa
      361 agccgcgcgt gaagaggtgg tcggccggcg cgcgcacgcc caggcgggtc agcgtcggcc
      421 cggcagtctg gctggcgtgg tcgaggtcgt ggtcggcata aagccgggcc agcgcccgcg
      481 cgccccggcc ggtgacgacg ttgaaatcgc cgccgatgag caccgccgtc gcgtcggcgg
      541 gcacgtccgt ggcaatggcg gccatctgcg ccgcgcgcag ggccaccggc gcggtggcga
      601 tctcgatatg ggtggagtag agcagcacct cgcgcccgcc gacgcgcgcc gtggctcgcg
      661 tggccgtgcg ctgctggccg ctcagggggt ggacgccggg taggatgagt ttgtgcgcgt
      721 cgctgagtgg ccagcgcgag aggatggcgt tgccgaagtc gtcgccatca ggggctaccg
      781 aggcggggaa gtagacataa tcgtaattca gcgccgcggc cagcgactcg gtgccggcgg
      841 catcgacctc ttgcagcagc aaaaagtcgg gcgcgggcag cggatcgcgg gcttcgaagg
      901 cgcggcgggc cgcatcgagg gcgtcgccaa agtggaggtt ataggagacg gccaggagtt
      961 cgtctggcgg ccgggcagcg gaggtggcgt gctggccacg ataaagaggg ccgtcactgt
     1021 cggtgtagtt gaccgtctgt ggcagtagca gacgcaaccc gccaaacgcg ctaagccgat
     1081 gcgccctctc acccacggcc ggcccgccaa gcgcatagac catcaccacc gacgcaagca
     1141 gggtcagcgg gccgaaaatc aacaggagaa acagcagggc acgccgccga cgacggttga
     1201 ggatcatgac aggttcgatg atgttgatct ctgtgtttcg ccgccggtcg cgccgtcggg
     1261 cagcagtact gtatcacgat cgccccgtcg ttgctggcca gcgggtcgcc gctcacgctc
     1321 ctctgaaaaa ttcccgcgcc gtctggccgc cgagcaggca ttgcctcaca caccggggtc
     1381 atcgccgcca tcccttgcgt cagagccaac gcgccagcgg aacgacagcc aaccttgccg
     1441 ccagaaccac caggacacca ggacagtcac accgatagac agcagcgtca tgaccacaaa
     1501 ggccggccgc gtgtctcgcc ccggcagggc gacgttcatg ccgtagaacg acgtgaacag
     1561 catcggcaca gtcaggatga tggtgaccgc cgccaggaac ttcatgacca cattgaggtt
     1621 gttggagatg agcgaggcaa aagtgtcggt catctcgccg gacacggcct gcatcgtcgc
     1681 cgccatgtgc gcgccctgcc gatactcgat ctcgacctcc ctgagcatct ccaggtcgtc
     1741 gtcggtccag ccaaagtgcg acccatcctg caaacgacgc accattgtct cgttccactc
     1801 cagcccggtc ttgatgtaca ccaggctctt ctcatagcgc agcaactcca tcatctcgcc
     1861 attctcgatg gagttgatca ggcggtcttc cacgtcgtcc atgagccggt cgatctcttc
     1921 cagatcgcgc agaaaccact ggccgacgat gcccaggatg gcgaggatca tgcggttggc
     1981 gcggcgcgtc ggcagggtgg tggcggagta gatggcggtc aggtcgtgga cgaaatcgat
     2041 tggccgccgg cagatggtca aaaagtggtt cggggcgatg atcataccca gcggcgtggt
     2101 gcgaaaaggc acgcgctcgc gcgccccgaa gttgtagggc acgcggacaa cggtcaggtg
     2161 catgccgcgt tgcgtatcgg tgcgggcgac ctcgcgcggg tcgacggccg cggccagaaa
     2221 gctcggcggc aggcccaact cgcgctgcaa caacgccagt tccacctctg agggcgactc
     2281 ggcatgaatc caggcccctg gggggaaccc gtcagccacc agtagcccgt tctcaccatc
     2341 acgatagatg ctgatcatag tcacctcact acgccgcaag tatagccgcg gaaagtggtt
     2401 tgtggatagg gcggggatgc caagtagggc gacgggtttg ttcggccggg gcgggcgcgg
     2461 ccagcgctac gcgccacgtg gccagcgcta cattgcagac gcaccctggt aggggctgac
     2521 cgccaactgc cggcggccaa ccacgaacca ctggccctgt cccctttccc cacctttcgt
     2581 ctataatcga cgaatagaca acagcgagca cggaggccct cctgaccact tacctgaccg
     2641 acaccgatca ccagtccgtc ctcgacctca tctggtcgca gatgccggcc aagcaaaacc
     2701 gtcatgcctc gtcgtggtgg ttcttcttgc tgtgccccga agggccagat agctacggcc
     2761 cgcgccagct catgttcacc gtcgccgcgc gcgccggcca gcgcattcgc atcaacgacg
     2821 tctggctgcc agggctagac cgggcgcggc ccgtcaccgg cggcgtggat cgcttcgacg
     2881 ccatgtctgt cggttggtac tgcgacggcg aggccgtcca tgacctgttg cggctggccg
     2941 gccgggcgga aatggacgcg accgcgggca gcttgcgctg gggcgacggc cacggccacg
     3001 gcgtcacctt tcgccagtcg gccgcgcgcc ctgtcggcct ggcggcgacg gtttgcggcc
     3061 ccggcggcgc ggccgagttc gaggcgtgga gcacgctcga ttccgcctac gacgcgccga
     3121 ccgtgtccat gcacatcgac acgcctttcg gcggcacgca ctacattggc tggcggcggc
     3181 tcaactttcg cgggcgcttc gagttgccca ccggggttga gacgctgcaa ggcacgggct
     3241 tctttcaacg tgtctgcctc aacgtacccg tcttcccatg gaagtggatt tggaccatct
     3301 tccccgaccg cagcgtcttc actgcctatt tgccctacgt cggcctgaat ctgttccgca
     3361 aaggctaccg cttcttccgc agcgagcggc tggaacaggc cgcgctgtcg atcaagccgt
     3421 cagcgatgtg ggttccggct ggcgggacgc ccatccgctt cgaccgcgtc agcgccttac
     3481 cccgcctcgg ccgcggcccc tacccgcaat tcgacgttcg cgccaccaac gccgccggcg
     3541 acgccatcgc cttcaccgcc gcgccctatg gcctgtcgcg cttctatatc gaccggccgg
     3601 tgctgggcgg gctggtcgag acacactgga actacaatga gttcatgttc cgcatggacg
     3661 acctggccgg ttccatcgcc ggccgctcgc tctcccgcgc caccatgggc caggcctatg
     3721 gcagcttcga gtatgcctat gggctggggc tgtaagagcc gaccacagac cgccgactac
     3781 ggacgacaga cgactgagcg ctgacctact gaccacatga tgccctcctc cccccgaaaa
     3841 cccgactgga cgcccgacga cgccggcgac gcctttaccg cgcgcatcat ctccggcgtg
     3901 gccggccggc acgatggcct ggacgccggc cgtgacgccg ccacgccgcc ggtcgcggcg
     3961 acccgcggcc cggcccgccg gccgctcagc atcgacgact acgttgccgg cgtcctgtcc
     4021 ggcgaccgcg ccgtgctggc ccgcgccatc accctgatcg agagcaatgc tccgacccac
     4081 tttgaaacgg cgcaggaggt tttgcgccgg ctgctgcccc acagcggcaa cgccttgcgc
     4141 gtcggcatca ccggtgtgcc cggcgcgggc aagagcacgc tcatcgaggc gcttggcctg
     4201 gccctcatcg ccgccggcca ccgcgtcgct gtcctggcca tcgacccatc cagcagcctg
     4261 acccgtggca gcattctggg cgacaaaacg cgcatggagg gactgagccg cgtcgccgag
     4321 gcgttcatcc ggccgtcggc caccggcggc gtgctgggcg gcgtggcccg caagacgcgc
     4381 gagacgatgc tcgtctgcga ggcggccggc ttcgatgtca tcctcatcga gacggtcggc
     4441 gcgggccaga acgaagtcac cgtccgctca atggtcgatt tctttctgct ggtgctcatc
     4501 cccggcgggg gagacgagtt gcagggcatc aagaagggcg tcgtcgagct ggccgacgcc
     4561 attgccatca acaaggccga tggcgacaac cgcgttcgcg ccgaggcggc gcgggcggaa
     4621 tataaccggg cgctacatta cctgacgccg gcgaccgaca gctggcacgc gcgcgcctac
     4681 acttgctcgg ccgagacggg cgagggcgtg gctaacctgt gggcggtcgt ggagcgcttc
     4741 cgcgagcgca cgaccgagtc gggcgcgttt gccgcccgcc gccgggcaca ggcgcgcgag
     4801 tggctacaca ccctgctcga cgagcaactg cgggccgcct tctacgaccg tcccgccgtc
     4861 cgcgccgccc tgccgcccat ggaggccgcg gtgatggctg gcgaactctc ggccacggcc
     4921 gccgcgcggc aactgctggc gctgctggac gggtagcccg ccactctcgc cgccaccagt
     4981 gtctggcact ttcttgaacg ccccactgcc ctaaccaccc ccaggaaggt gcgacccacc
     5041 gggcgcttgc gctatactca cccggcgatg gtcacttcct ctcccgcgc
//
LOCUS       DAOSMQ010000217         5076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16623_length_5076_cov_3.656642, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000217 DAOSMQ010000000
VERSION     DAOSMQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16623_length_5076_cov_3.656642"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PK829_08340"
     CDS             <1..576
                     /locus_tag="PK829_08340"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOU41261.1"
                     /translation="ADKGMYAALLMAVTRTLFMQEAQRSRSPAEVALAVHRGLLTVGM
                     AEDSQGMDAFVTAFYGVLHRPTGRLTYVRAAQDYPLLFRPGQEPVSLPGIGRFLGMIA
                     DLSLEEQVVTLRPGDYLLLYSDGVTDARNMAGDSYGLARLKHVFQSTAGQSAARCLEQ
                     VAADINAWRGEAAAFDDVTMLLVEAVTAGHQ"
     gene            581..1117
                     /locus_tag="PK829_08345"
     CDS             581..1117
                     /locus_tag="PK829_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU41262.1"
                     /translation="MLRKSTPSSHSSEPAERSQPWLWLGLGTLITVIGLALALWALAG
                     FLSQPPAATSAGLEPTIIRLTAPPVPTATAAIFVAPPTAAPTATSVPTPDLSVAPPEV
                     TAGYYATVVETGGVGVTVRNGPNTSNAPITVAGENSVALVIEGPTPGGAYQWWRIRLP
                     DGTEGWVAGDFLSPAAAP"
     gene            1160..3181
                     /gene="ligA"
                     /locus_tag="PK829_08350"
     CDS             1160..3181
                     /gene="ligA"
                     /locus_tag="PK829_08350"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOU41263.1"
                     /translation="MTPEERIQQLRRELNYHLYRYHVLDAPVISDAEYDALYRELVAL
                     EAEHPELATPDSPTQRAGAAAADIFAKVRHPAPILSLMNAFSADDLRAWRTRIGRLLP
                     ADTHLEYVVEPKLDGLTVVLHYRDGRFVQGATRGDGEVGEDVTANLRTLHGVPKHIPV
                     DPQQTLRPPDYLVVRGEVFLPLDKFEALNAERLAAGEPLYMNPRNTAAGSLRQLDPAK
                     TAERPLRLYCYDIVAIEGGELPRRQWQRLAYLRDLGFPVSPDNTYCRSLNEVVAAYEA
                     WIERRDHINYEVDGAVVKVDDRLLAEELGYSGKDPRGALAMKFPAREKTTKLLALQVN
                     VGRTGVLAPNAVLEPVEIGGTTVRAATLHNFDEIARKDIRIGDRVLIKRAGDVIPYVV
                     GPIVDIRDGSEQPIVPPQTCPACGQPVVQLPDEVAYYCENATCPAQIIRRVEYFVSRG
                     AMDIDGFGAQTAALLCERGLIHDVADIYHLRREDLLELEGFGDKKVDRLLAGVEASKE
                     RPVDRLLTALGIRFVGGVVAELLINSLGSLDALATAGADVLDDITGVGPRTAVSVAAW
                     FANEGNRELIAKLRAAGLRMATDRAATPQASQTLAGQSFVLTGTLPGLTREEATALIE
                     AHGGKVSGSVSKKTSYVVAGEAAGSKLDRAHVLGVPVLDAAGLRALVGE"
     gene            3194..4447
                     /locus_tag="PK829_08355"
     CDS             3194..4447
                     /locus_tag="PK829_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOU41264.1"
                     /translation="MTEFFNVLPPDDARALLLSHLTRPVAAETIATSEALGRVTAQAV
                     RAPHALPTFRRSTMDGYAVRAADTHGAGETLPALLQAVGEVSMGQKAGVALRRGEAVL
                     IHTGGMIPDAADAVVQVEHTQRIEGAGGADFPYEIEVLRPVAVGQNVIQVGEDVAASA
                     TVLPAGHRLRPQDLGGLVALGLTTVAVACRPRVAILATGNEVVPPDVTPGPGQVRDIN
                     TYTIAAQIQAAGGIPEPLGIIADDAAALRAAAESALARSDMVVITAGSSVSARDMTVT
                     IIAALGAPGVLLHGVATRPGKPTIVGAVAGKPVLGLPGNPVSALIQFDMFGVPALYHL
                     QGLVGERPRNRVWARLAANVPSASGREDYVPARLEESAGELLAVPVFGKSNLIYTLVG
                     ADGLIVVPLNRAGLMAGDPVEVRLF"
     gene            complement(4444..>5076)
                     /locus_tag="PK829_08360"
     CDS             complement(4444..>5076)
                     /locus_tag="PK829_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU41265.1"
                     /translation="FLNAIGGQAALCQAQPCLDVDQSRIDWQDGVAVVAADREQFPAA
                     GVTWYGAAAYCAWREARLPTEAEWEKAAGWDDSTMTARRYPWGDEFHGKRLNFCDATC
                     DAPQANLSYNDGYAVAAPVGSYADGRSATGHDDLAGNVWEWVADWFSPDYYATSPGAD
                     PTGPESGEQKVVRGGSWFDTAYFTLTTVRQSSPPENADATLGFRCAANTP"
BASE COUNT          785 a         1720 c         1767 g          804 t
ORIGIN      
        1 gccgacaagg gcatgtacgc tgctctgctg atggccgtca cgcggacgtt gttcatgcag
       61 gaggcgcagc gttcgcgctc gccggccgag gtggctctgg ctgtccaccg cggcctgctg
      121 accgtcggca tggccgagga tagccagggc atggacgctt ttgtcacggc gttctatggc
      181 gttctccacc ggccaactgg ccgcctgacc tacgtccgcg ccgcccagga ttaccccttg
      241 ctgttccggc ccgggcagga gccggtgtcg ttgcccggca tcggccgctt tctgggcatg
      301 atcgccgatc taagcctgga ggagcaggtc gtcaccttgc gaccgggcga ttatctactg
      361 ctctatagcg acggcgtcac cgacgcgcgc aatatggccg gcgacagcta tggcctggcg
      421 cggctgaagc acgtctttca gtctaccgca gggcagagcg cggcgcgttg cctggagcag
      481 gtggccgccg acatcaatgc ctggcgcggc gaggcggccg ctttcgacga cgtgaccatg
      541 ctgctggtgg aggcggtcac cgccggccac caataaccgt atgcttcgga aatcaactcc
      601 ctcatctcat tccagcgaac cggcagagcg aagccaaccc tggctgtggt tggggttggg
      661 cacgctcatc accgtcattg gtctggccct ggccctgtgg gcactggccg gcttcctgtc
      721 ccagccgccg gcggcaacat cggccggcct ggagccgacg atcatccgtc tgacggcccc
      781 gcccgtgccc acggcgacgg cggccatctt tgtcgccccg cccacggccg cgccgacggc
      841 cacgagcgtg cccacgcccg acctgtccgt ggccccgccg gaggtgaccg ccggctacta
      901 cgccaccgtc gtcgagacgg gcggcgtcgg cgtcaccgtg cgcaacgggc cgaacaccag
      961 caacgcccct atcaccgtcg ccggcgagaa ctcggtcgcc ctagttattg aagggccgac
     1021 gcccggcggc gcttatcagt ggtggcgtat ccgcctgccc gatggcaccg agggctgggt
     1081 ggcgggcgac ttcctgtcgc ccgcggccgc gccgtagccg ggtgaatggt ggtggacaaa
     1141 cacaccgata ggcaaccgta tgaccccaga agaacgtatt caacagctac gccgggagct
     1201 taactaccat ttgtatcgct atcacgttct cgacgcgccg gttatcagcg acgccgagta
     1261 cgatgcgctc taccgcgagt tggtggcgct ggaggccgag catccggagt tggcgacgcc
     1321 cgactcgccc acgcagcgcg ctggcgcggc cgcggccgac atcttcgcca aggtgcgcca
     1381 ccccgcgccc atcctcagcc tcatgaacgc cttcagcgcc gacgacctgc gtgcctggcg
     1441 cacgcgcatc ggccggctgt tgcccgcgga cacccatctg gagtacgtcg tcgagccgaa
     1501 gctggatggg ctgacggtcg tgctccacta ccgcgatgga cgctttgtgc agggggctac
     1561 ccgcggcgac ggcgaggtgg gcgaggatgt caccgccaac ctgcgtacgc tgcatggcgt
     1621 acccaaacat atccccgtcg atccccagca gacgctccgg ccgccagatt acctggtcgt
     1681 gcgcggcgag gtcttcctgc cgctggataa gttcgaggcg ctaaacgctg agcgactggc
     1741 cgccggcgag ccgctctaca tgaacccgcg caacaccgcc gccggttcgc tgcgccagct
     1801 ggacccggcc aagacggccg agcggccgtt gcgcctctac tgctatgaca tcgtggctat
     1861 tgagggcggc gagttgcccc gacgccagtg gcagcggctg gcctatttgc gcgatctggg
     1921 cttccccgtc tcgcccgata acacctactg ccgcagtctg aacgaggttg tggccgccta
     1981 tgaggcgtgg atcgagcgcc gcgaccacat caactatgag gttgacggcg ctgtcgtcaa
     2041 ggtcgatgat cgtttgctgg ccgaggaact gggctattcg ggcaaggatc cgcgcggcgc
     2101 gctggcaatg aagttcccgg cgcgggaaaa gacgacgaag ctgctggcgc tgcaagtcaa
     2161 cgtcggtcgc accggtgtgc tggcccccaa cgctgtcctc gagccggtcg agatcggcgg
     2221 cacaacggtg cgcgcggcga cgctgcacaa cttcgacgag atcgcccgca aagacatccg
     2281 catcggcgac cgcgtgctta tcaagcgggc gggcgatgtg attccttacg tcgtcgggcc
     2341 aattgtggat atccgcgacg gcagcgagca gcctattgtt ccgccgcaga cgtgcccggc
     2401 ctgcggccag ccggtcgtgc agttgccgga cgaggtggcc tactactgcg agaacgccac
     2461 ctgcccggcg cagataatcc ggcgcgtaga gtacttcgtc agccgcgggg ccatggatat
     2521 cgacggcttt ggcgcgcaga cggcggcgct gctgtgcgag cgtggcctca ttcatgacgt
     2581 ggccgacatc taccacctgc ggcgtgaaga cctgctcgaa ctggaaggct ttggcgacaa
     2641 gaaggtcgat cgcctgctgg ccggcgtcga agccagcaag gagcggcccg tcgaccggct
     2701 gctgacggcg ctgggcattc gcttcgtcgg cggcgtcgtg gccgagctac tgatcaacag
     2761 cctgggcagc ctcgacgcgc tggccacggc cggcgcggac gtactggacg acatcaccgg
     2821 tgtcgggccg cggacggccg tatcggtggc ggcctggttt gccaacgagg gtaaccgtga
     2881 gttgatagcc aagctgcgcg cggccgggct aaggatggct acggacaggg cggcgacgcc
     2941 gcaggcgagc cagacgctgg ccgggcagag cttcgtgctg accgggacac tgccgggcct
     3001 gacgcgcgaa gaggccacgg cgctgatcga ggcccacggc ggcaaggtca gcggctcggt
     3061 cagtaagaag accagctacg tggtcgccgg ggaggccgcc ggcagcaagc tcgaccgcgc
     3121 ccatgtgctg ggcgtaccag ttctagacgc tgccggtctg cgggcactgg tgggcgagta
     3181 ggcgcagccg ggcatgaccg agttcttcaa cgtcctgccg cccgacgacg cccgcgccct
     3241 gctgctgtcg catctgacac ggccggtggc ggccgagacc attgccacga gtgaggcact
     3301 cggccgcgtg acagcgcaag cggtgcgcgc cccccacgct ctgcccacct ttcgccgcag
     3361 cacgatggac ggttacgccg tgcgcgccgc cgacacccac ggcgcgggcg agaccttgcc
     3421 ggccttgtta caggcggtgg gcgaggtgag catgggccag aaggccggcg tagccctgcg
     3481 ccggggtgag gcagtgctca tccacaccgg cggcatgatc cccgacgcag ccgacgccgt
     3541 ggtgcaggtg gagcacaccc agcgcattga gggagccggc ggcgcggatt tcccctatga
     3601 gatcgaggtg ctgcggccgg tggctgtggg ccagaacgtc attcaggtgg gcgaggacgt
     3661 ggctgcgtcc gcgacggtgc tgccagccgg ccaccgcctg cggccgcaag acctgggcgg
     3721 cctggtggcg ctggggttga cgaccgtggc cgtggcctgc cggccgcgtg tggccatcct
     3781 ggccacgggc aacgaggtcg tgccgccgga cgtgacgccg gggccgggac aggtgcgcga
     3841 catcaacacc tacactatcg ccgcgcagat acaggctgcc ggcggcatcc ccgaaccgct
     3901 cggcatcatc gccgacgacg ccgcggccct gcgcgccgcg gccgagagcg ctctggccag
     3961 aagcgatatg gtggtcatca ccgccggctc ctcggtcagc gcccgcgata tgaccgtgac
     4021 gatcatcgcc gccttaggcg cgccgggcgt gttgctccac ggcgtggcca cgcggccggg
     4081 caagccgacc atcgtcggcg cggtggcggg caagccggtg ctggggctgc cgggcaaccc
     4141 cgtctcggcc ctgatccagt tcgacatgtt cggcgtgccg gcgctctatc acctgcaagg
     4201 gctggtggga gagcggccgc gcaaccgcgt ttgggcgcgg ctggcggcca acgtgcccag
     4261 cgccagcggg cgcgaggact atgtgcccgc gcggctggag gaatcggccg gcgaactgct
     4321 ggccgtgccc gtctttggca aaagcaacct catctacacc ctcgtaggcg cggacgggct
     4381 gatcgtcgtg ccgctcaacc gtgccgggct gatggccggc gacccggtcg aagtgcggct
     4441 gttctagggc gtgttggcgg cgcagcgaaa gcctagcgtg gcgtcggcgt tctccggcgg
     4501 tgacgactgg cgcaccgtgg tcagggtgaa gtaggccgtg tcgaaccagg agccgccgcg
     4561 cacgaccttt tgttcgcccg actccggccc ggtggggtcc gcgcccggcg aagtggcgta
     4621 gtagtccgga gagaaccagt cggctaccca ctcccaaacg ttgccggcca ggtcatcgtg
     4681 gccggtggcg ctgcggccgt cggcgtaact gcccacaggc gcggcgacgg cgtagccgtc
     4741 attgtaggac agattggcct ggggcgcgtc acaggtcgcg tcgcagaaat tcagccgctt
     4801 gccatggaat tcgtcgcccc aggggtagcg gcgagccgtc atcgtgctgt cgtcccagcc
     4861 agcggccttc tcccactcgg cctcggtcgg cagccgcgcc tcgcgccagg cacagtaggc
     4921 cgccgcgcca taccacgtta cccccgcggc cggaaattgc tcgcggtcgg cggcaacgac
     4981 ggccacgccg tcctgccaat cgatgcggct ttggtctacg tcgaggcatg gttgtgcctg
     5041 gcagagagcc gcctggccgc cgatggcgtt caggaa
//
LOCUS       DAOSMQ010000218         5075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16624_length_5075_cov_5.357968, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000218 DAOSMQ010000000
VERSION     DAOSMQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16624_length_5075_cov_5.357968"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PK829_08365"
     CDS             complement(<1..328)
                     /locus_tag="PK829_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41266.1"
                     /translation="MNPAIAVPAFNRPASLQRLLRALAAADVDAGTPLLLSIDCPADD
                     RHAAANAAVAAVARAFRWPYGPLEIVAQPAPLGLVGNVFFCGAAAESYGAVVLLEDDL
                     LVSARFH"
     gene            complement(325..1686)
                     /locus_tag="PK829_08370"
     CDS             complement(325..1686)
                     /locus_tag="PK829_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU41267.1"
                     /translation="MRRILAARNSSYPRPSAQPAFPSAAQPSPRPGWLGRIGLSSFWL
                     LAARVLGQGLGLLFTAFLARALGEEGLGQFAFLSAVLFIGNAATTFGLDTVLLREVAT
                     GRRDAAVVDSGATAGAVLLIQMALSAAFIAALWLVMPWLPNQTAATLPAVRLVSLALI
                     PLAFSTVYSAVLRAHERMAHYMFFTLVVAVALALGGLAVWAGGGGLRAAAVVVVLAQS
                     GGALAAAGLARVALPGAAWRWARPPAAVVRRVAQLGAGLAALMVLSILYQRSGVLLLS
                     LLAGDRAAGAYSASARVVEALKMLPAAFFGAMFPIMAQGRGDRDAERAFGRAFAALMA
                     LVVVLAAVTGLAAGPLLALLFGRGYEAAVTPLRIMVWSLPATLLAFRLSFELVVAGRD
                     RAAATSMGLTLLLGGGLTAALISRWSLAGAAVGLVSGEIVQVAVLLVVGRLGRASGRE
                     VSQ"
     gene            complement(1726..2565)
                     /locus_tag="PK829_08375"
     CDS             complement(1726..2565)
                     /locus_tag="PK829_08375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41268.1"
                     /translation="MSQPPLPAAPGDQGQAHYDNVAEAFSRKSTIYDAFGENHANMAR
                     MRAKFYAHMEAVMPPGGHLLEINAGTGQDAVALVERGFRVHATDFAPGMVAAIHEKIA
                     RYDLGQRLTVEALSFTDLDRLSLGPFDGLYSNSGGLNCIADLTVVTRQLPRLLQPGAR
                     VTWVIMPRICPWELAVIPKDPRVGTRRLRPGGIMAHVEGVHFRTYYFSARTVRRAFGP
                     RFRPVRQEGLSVFTPTADNKTFAVNHPRLFDRLARLDDALAPRPPFNGWGDFFILTME
                     FLG"
     gene            complement(2562..4100)
                     /locus_tag="PK829_08380"
     CDS             complement(2562..4100)
                     /locus_tag="PK829_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU41269.1"
                     /translation="MVDILFGQSYYLRFDPKLWAAQQPYPPLGTLYAAAVMRQAGYSV
                     ALFDAMLAESEAEWAAALDAHQPRFAVIYEDNFNYLSKMCLLRMREAAFTMSAMAAAR
                     GCVVIVAGSDASDHSAAYLRSGAHYVLLGEGEETLTQLLDRLTGRTATPLSDIRGLAW
                     PEGDQIESNPRRAPIADLDALPFPAWDLVDVERYRRVWQTHHGRYSMNMVTTRGCPYH
                     CNWCAKPIWGQRYHSRSPECVAEEMGWLKEAYRPDHIWFADDIVGLKPGWWTRFAAAV
                     DARGAQVPFKCLSRADLIMRNEADVLALARAGAEIVWIGAESGSQDVLNAMEKGTTVQ
                     QIRDATRRLHDAGVRVGFFLQFGYPGETRDDIEATLQMVRDCRPDDIGMSVSYPLPGT
                     KFHANVRAQLSDKQNWIDSSDLDMLYQGPFSSDFYRQLHVVLHKEFRARWALDKARQV
                     AARPGQWRPGHLRGLGGAVARLVTLPAARARLDRLAAAPSVAIQPARAMPYEEAARPT
                     PQPE"
     gene            complement(4102..5022)
                     /locus_tag="PK829_08385"
     CDS             complement(4102..5022)
                     /locus_tag="PK829_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41270.1"
                     /translation="MNTSRFDVAQAIVNTVSYVDVFDYPLTPAEIHRYLIRLPLTRQD
                     TQHFLHEGGLVPEHLSQRDGLYMLPGREAVAEVRQRRQEASRELWPAAVAYGHLIAGM
                     PFVRLVAITGSLSVNNVNRGGDIDYLVVTANDHLWVSRAFVLLIVRWAARRGHEVCPN
                     YFLSERALRLSDRSLYTAHEIAQMVPLYGRRVYDALRQANDWTREYLPNAAGPPATLR
                     DAETYLRRLGPSPNHRLLEATLATPPGLRLDRWEMRRKIDKFRRVYQGWQESDFSPDM
                     CKGHFQRHQQKVAAAYAKHALASGKRVGLG"
BASE COUNT          910 a         1644 c         1784 g          737 t
ORIGIN      
        1 cgtgaaagcg ggcggagacg agcagatcat cttccagcag cacgaccgcg ccatagctct
       61 cggccgccgc gccgcagaag aaaacgttgc ccaccaaacc aagcggcgcg ggctgcgcga
      121 cgatctccag cgggccgtag ggccaacgaa aggcgcgcgc gaccgcggcc acggccgcgt
      181 tggctgcggc gtggcggtcg tcggcggggc aatcgatgga gagcagcagc ggcgtgcccg
      241 cgtcgacatc agcggcggcc agagcgcgca atagtcgctg tagcgaggcc gggcggttga
      301 aggcgggcac ggcaatggcg gggttcattg gctcacttcc ctgccagacg cgcggccgag
      361 acggcccacg acgagcaaga cggccacttg cacgatctcg cccgacacca gcccgacggc
      421 cgcgccggcg agcgaccagc ggctgatcag cgctgctgtc agcccaccgc cgagcagcag
      481 ggtcaggccc attgacgttg ccgcggcccg gtcgcgtccg gcgacaacaa gctcaaacga
      541 caggcggaag gccagcagcg tcgccggcag gctccagacc atgatgcgca gtggcgtgac
      601 agcggcctca tagccgcggc caaagagcag cgccagcaac ggcccggccg ccagcccggt
      661 gactgcggcc agcaccacga ccagcgccat gagtgcggcg aaagcccggc cgaaggcgcg
      721 ttcggcgtca cgatcgccgc gcccctgggc catgatggga aacatggctc cgaagaaggc
      781 cgctggcagc attttgagcg cctccacaac gcgggcggag gcactgtagg ctccggcggc
      841 gcggtcgccg gccagcaacg acagcagcag aacgccgctg cgctggtaga ggatcgacag
      901 caccatgagc gccgccaacc ccgcccccag ttgggccaca cgcctgacta cggctgccgg
      961 cggccgcgcc cagcgccacg ccgcgccggg caaggcaacg cgcgccagcc cggccgcggc
     1021 caatgcgccg ccgctctgag ccagcacgac gacgacggct gccgctctca gcccgccgcc
     1081 accggcccag accgccagac cgcccagcgc cagggctacg gctacgacca gcgtaaagaa
     1141 catatagtgg gccatgcgct cgtgggcgcg caggacggcg ctgtagacgg tggaaaaggc
     1201 cagcgggata agggccagcg agacaaggcg cacggccggc agcgtcgccg ccgtctgatt
     1261 gggcaaccag ggcattacca accagagggc ggcgataaac gcggccgaca gagccatctg
     1321 gattagcaga accgcgccgg ccgtcgcccc gctatcgacc acagcggcat cccgccggcc
     1381 tgtggccact tcgcgtagca gtacggtgtc gagaccgaag gttgtggccg cgttgccgat
     1441 gaaaagcacg gccgagagaa aagcaaactg ccccagcccc tcttcgccca gcgcccgcgc
     1501 caggaaggcg gtgaacagta gccccagtcc ctgccccagg acgcgcgccg ccagcagcca
     1561 gaacgagcta agcccaatgc gccctaacca gccgggccgt ggcgacggct gcgccgcgga
     1621 aggaaaggcc ggttgggccg atggacgcgg ataggaactg tttcgggccg cgagaatcct
     1681 tctcatccgt gtcgtccgcg cctgatcggg ccgaaggatc gtcgcctagc cgagaaattc
     1741 cattgtcagg atgaagaagt cgccccagcc attgaacggc ggccgtggag cgagagcatc
     1801 gtcgagccgc gccagccggt cgaagagacg cgggtggttg acggcgaatg tcttgttgtc
     1861 ggccgtcggt gtaaagaccg acagaccttc ctgccgcacc ggccggaagc gtggcccgaa
     1921 cgctcgccgc accgtccggg cgctgaagta gtaggtccgg aagtggacgc cttcgacgtg
     1981 ggccatgata ccgccggggc gcagacggcg cgtgcccacg cgcgggtcct tggggatgac
     2041 ggccagctcc cacgggcaga tgcgcggcat aatcacccag gtgacgcgcg cgcccggctg
     2101 caacaggcgc ggcagctgcc gcgtgaccac ggtcaggtcg gcgatgcagt tcagcccgcc
     2161 ggagttggag taaaggccat cgaacgggcc gagcgacagc cggtcgaggt cggtgaacga
     2221 cagcgcctcg actgtcagcc gctggcccag atcataccgg gcgattttct catggatggc
     2281 cgcgaccatg ccgggagcga agtcggtggc gtggacgcgg aaaccgcgct cgaccagggc
     2341 cacggcatcc tggcccgtgc cggcgttgat ctccagtaag tgtccgccgg gcggcatgac
     2401 cgcctccatg tgggcgtaga acttggcccg catgcgggcc atgttggcgt ggttctcgcc
     2461 aaaggcgtca tagatggtcg acttgcgcga gaaggcctcg gcgacattgt catagtgggc
     2521 ctggccctgg tcgccggggg cggcggggag aggaggctgg ctcattccgg ttgtggggtg
     2581 gggcgggcgg cttcttcgta gggcatcgct cgcgctggct ggatggcgac tgagggcgcg
     2641 gcggccagcc gatccagccg ggcgcgggcc gccggcagcg tgaccaggcg ggcgacggcc
     2701 ccgcccaggc cgcgcaggtg gcccggccgc cactgccctg gccgcgccgc cacctgccgc
     2761 gccttgtcca gcgcccaccg ggcgcgaaac tccttgtgca gcacgacgtg aagctggcgg
     2821 tagaagtcgc tgctgaaggg gccttgatag agcatgtcca ggtcggatga gtcgatccag
     2881 ttctgcttgt cgctgagttg ggcgcgcacg ttggcgtgga acttcgtgcc tggcagggga
     2941 taggagaccg acatgccgat gtcgtccggg cggcagtcgc gcaccatttg cagcgtcgcc
     3001 tcgatgtcgt cgcgcgtctc gccggggtag ccaaattgca ggaagaagcc gacgcgcaca
     3061 ccagcatcat gtagccgccg ggtggcgtca cgaatctgct ggacggtcgt gcccttttcc
     3121 atggcgttaa ggacgtcctg cgagccggac tccgccccga tccagacaat ttcggccccg
     3181 gcgcgggcca gggcgagcac atcggcctcg ttgcgcataa tcaagtcagc gcggctgaga
     3241 catttaaacg gcacttgcgc cccgcgcgcg tctacagccg cggcgaagcg cgtccaccag
     3301 ccgggcttca ggccaacgat gtcgtcggca aaccagatgt ggtcgggccg gtaagcttct
     3361 ttcagccagc ccatctcctc ggcgacgcac tccgggctgc gcgagtggta gcgctggccc
     3421 caaatgggct tagcgcacca gttgcagtga tagggacagc cgcgcgtcgt gaccatgttc
     3481 atcgagtagc ggccgtggtg ggtctgccat acgcgccggt agcgctcgac atcaaccagg
     3541 tcccaggcgg gaaaaggcag ggcatcgagg tcggcaatgg gcgcgcgacg tggattgctc
     3601 tcgatctggt cgccctcggg ccaggccaga ccgcggatgt cgctcagggg cgttgccgtt
     3661 cggccggtta gccggtcgag cagttgagtc agggtctctt cgccctcgcc cagcaagaca
     3721 tagtgcgccc cggagcgcag ataggcggcg ctgtggtcgc tggcgtcgga gccggcgacg
     3781 atgacgacgc agccgcgggc cgcagccata gccgacatcg tgaaggccgc ctcgcgcatc
     3841 cgcagcaggc acatcttgga cagatagttg aagttgtcct cgtagatcac ggcgaagcgc
     3901 ggctggtggg cgtccagcgc cgcggcccac tccgcttcgg actcggccaa catggcgtcg
     3961 aacagagcta cggagtagcc ggcctggcgc atgactgccg cggcatagag tgtgcccagt
     4021 ggtgggtagg gctgttgcgc cgcccagagc ttgggatcga agcgcagata gtatgactgg
     4081 ccaaagagga tatcaaccat attaacccag gcctacccgc ttgcccgacg ccagagcatg
     4141 tttggcatag gcagccgcga ccttctgctg gtggcgctga aaatgccctt tgcacatgtc
     4201 gggcgagaag tctgactcct gccaaccctg atagacccgc cggaacttgt cgatcttgcg
     4261 cctcatttcc cagcggtcaa ggcgcaggcc gggcggcgtg gccagggtgg cctccaggag
     4321 acggtggttg ggcgaggggc ccagccggcg cagataggtt tccgcgtccc gcagcgttgc
     4381 tggcgggccg gcggcattgg gcaggtattc gcgcgtccag tcgttggcct ggcgaagcgc
     4441 gtcgtagacc cggcggccgt agagcggcac catctgggca atctcgtggg cggtgtacag
     4501 gctgcgatca ctcaggcgca aggcccgctc gctcaggaag tagttggggc aaacctcgtg
     4561 gccacggcgg gcggcccaac ggacgatgag taacacgaag gcacgcgaga cccagaggtg
     4621 gtcgttggcg gtgacgacga gatagtcgat gtcaccgccg cggttgacgt tattgaccga
     4681 cagcgacccg gtgatggcta cgaggcgcac aaacggcatc ccggcaatga ggtggccata
     4741 ggccacagcc gccggccata attcgcgcga ggcctcctgg cggcgctgcc gcacttcggc
     4801 cacagcctcg cggcctggta gcatatacag gccgtcgcgc tggctgagat gttccggcac
     4861 aaggccgcct tcgtgcagga agtgttgcgt gtcttgtcgc gtgagtggaa ggcgaatgag
     4921 gtagcggtga atttcggccg gcgtcaacgg gtagtcgaat acgtccacgt aggacaccgt
     4981 gttaacgatt gcctgtgcca cgtcgaaccg ggaagtgttc atcggttgcg ctaaggtaca
     5041 actggttacg ggctggggcc tagtggactt agccc
//
LOCUS       DAOSMQ010000219         5055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16704_length_5055_cov_4.218600, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000219 DAOSMQ010000000
VERSION     DAOSMQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16704_length_5055_cov_4.218600"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1585)
                     /locus_tag="PK829_08390"
     CDS             complement(155..1585)
                     /locus_tag="PK829_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOU41271.1"
                     /translation="MTVKFGTDGWRAIISDEFTFDNLRLVAQAIADYVHSQGAAAPSV
                     VIGFDTRFLSDRYAAEVARVMAANDIHTWLARADTPTPAISYAVVNKGATAGVMITAS
                     HNPPRYNGVKLKAAYGGSASPDEHRQVERLLMTNAAEGRAPRLMNLDQALREDRVVKF
                     DPAWAYYEHLGTLIDLDIIHEGELRVIHDAMYGAGRRTISEMLMRTRTHVQNMRDEMN
                     PGFGGIHPEPIAKHLGLLASAMSAGNWHAGLATDGDADRIGAVDARGQFVDPHRIFGL
                     ILRHLLHHRGLRGPVVRTVSTTRMIDRIAAECGLPVIETPVGFNHIADLMLEQDVVMG
                     GEESGGMSIHGHIPEGDGVLMGLLLLEVMAEAHKPLHILVDELLTTYGPAQYARTDMK
                     LRRPVVKAELVDKLVSRAPDAIAGVAVEEVRSSDGIKYYLADGSWLLIRPSGTEPVLR
                     VYAEAPSDARVSALLGFGEVIAEGEH"
     gene            complement(1660..3030)
                     /locus_tag="PK829_08395"
     CDS             complement(1660..3030)
                     /locus_tag="PK829_08395"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOU41272.1"
                     /translation="MTATASISPRFADYRRHADELVGATIAAADPNAAVSRFLRREGR
                     TLWVGRASEAEAIDLDRGRIYLVAAGKAAISMTTAAMAVLGADVAGGVVIAKVGGHEW
                     PEVAGQWPLALHRGGHPLPEAGSLAGTKAVLELLAATRAEDAVLCLISGGASSLLTQP
                     LLPLEDWRKLVEVFLKSGCTISELNAVRRAIDRIKAGGLARACAPASCYGLILSDVVG
                     NPLPVIGGGPTVPDDDLVVGAVAVLGRYDIARQLEKAEWQRLALALNQARYLHKAARP
                     SVRNFIVGDVREAATAALATAIRLGFVGQLLTAHLDGEARAIGRLAAGIARDLPPGQC
                     YIMGGETTVTVRGKGRGGRNQELALAAALALERQPRAAVLSFSTDGEDGPTPAAGAIV
                     SGATAPLARSYGIDPLAHLADNNSYAFFSRLDEAARGQVDPHLVVTGPTGTNVNDLVV
                     ILSYAG"
     gene            complement(3027..3653)
                     /locus_tag="PK829_08400"
     CDS             complement(3027..3653)
                     /locus_tag="PK829_08400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU41273.1"
                     /translation="MSLPTRLLFIRHARSVWNEAGRWQGQADPPLSESGLAQAQRLAE
                     RLCRATAIEISHIYASDLQRAQGTATIIAAALGRPMTTDPVWRERAIGQLEGLTTDEI
                     ITRFPDAWASRHHGSMHAPGGESPDDVLRRAVSGCKAVLARHDGQTIAVVSHGGMILA
                     TLVHLLDFGPSGFMRLNGGNHTAISQVDIIDGLARLERLNDCAHLELP"
     gene            complement(3678..4343)
                     /locus_tag="PK829_08405"
     CDS             complement(3678..4343)
                     /locus_tag="PK829_08405"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOU41274.1"
                     /translation="MLIRLNAANRPNATPIIREDTISRDHLARLTWIVSAATGFFVLW
                     VLWRYGIAANAAARWPDWLGDLLDLFTRASLLTLTLLWALIVWQRRAQQRMEPTARMT
                     VEQLYALSPRAFEQFVAELFERRGYTVEVRGRAGDLGVDLALTRADGRRAVVQCKRYR
                     HQVGPDIIRELFGTMVHERAFHGFLVTTAEISEAARQWAADKPMTLIDGAALSALSDD
                     LRA"
     gene            4581..4781
                     /locus_tag="PK829_08410"
     CDS             4581..4781
                     /locus_tag="PK829_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41275.1"
                     /translation="MIITKRQLGGLFILLGVGVAVGLFAIDWLGAGQFQGIGPAQRRA
                     LLLAGGVVLLGLSLLPLGNRPA"
     gene            4801..>5055
                     /locus_tag="PK829_08415"
     CDS             4801..>5055
                     /locus_tag="PK829_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41276.1"
                     /translation="MSLHEIHDAWEVLLNTDATSAMPVAQAAPRTAALLPRLLIGLAL
                     AAFISYLVVYVIYAVQLLRFPFDYDQGEGFELMDTILFSHG"
BASE COUNT          818 a         1666 c         1720 g          851 t
ORIGIN      
        1 cgacttcatc aatggcatcc gccacagcgc gcggattctc taccgcttcg cgtctatgta
       61 gggtgaacgg cttgagccgc gggacgttgc gccccgcggc tcggcctcta agattctgct
      121 ccccctccct atgggagggg ttaggggagg gctactaatg ttcgccctcg gcgatgacct
      181 cgccaaagcc gagtagagcg ctgacacgcg cgtcactggg cgcttcggca tagacacgca
      241 gcaccggctc ggtgcccgac ggccggatga ggagccaact gccgtcggcc aggtagtact
      301 tgatgccgtc gctgctgcgc acctcttcga cggccacacc ggcaatggcg tcgggcgcgc
      361 ggctgaccag cttatcgacc aactcggcct tgaccaccgg acgccgcagc ttcatatccg
      421 tccgcgcata ctgcgccggg ccataagtgg tcagcaactc gtcgacgaga atgtgcaacg
      481 gcttgtgggc ctcggccata acctccagca gcagcaaccc catcagcacg ccgtctcctt
      541 ccggaatgtg gccgtggatg ctcatgccgc ccgactcctc cccgcccatg accacatcct
      601 gttccagcat gagatcggcg atatggttaa acccgacggg cgtctcgatg accggcagcc
      661 cgcactcggc ggcgatgcgg tcgatcatgc gcgtggttga gacggtgcgc acgactgggc
      721 cgcgcaggcc gcggtggtgc aacaggtggc gcaaaatcag gccaaagatg cggtggggat
      781 cgacaaactg gccgcgggca tcgaccgcgc cgatgcggtc ggcgtcgccg tcggtggcca
      841 gcccggcgtg ccagttaccc gcgctcatgg ccgaagccag cagacccagg tgcttggcga
      901 tcggctccgg gtggatgccg ccgaagccgg ggttcatctc gtcgcgcatg ttctggacgt
      961 gggtgcgcgt gcgcatgagc atctcgctga tggtgcgccg gcccgcgccg tacatagcgt
     1021 cgtggatgac gcgcaactcg ccctcgtgga tgatgtccag atcgatcagc gtgcccaggt
     1081 gctcgtagta ggcccaggct gggtcgaact tgaccacgcg gtcttcgcgc aaggcctggt
     1141 cgaggttcat gaggcgcggc gcacgcccct cggccgcgtt ggtcatcagc agccgctcca
     1201 cctggcggtg ctcgtccggg ctggcgctgc cgccataggc cgccttgagc ttgacgccgt
     1261 tgtagcgcgg tgggttatgg ctggcggtga tcatgacgcc ggccgtggcc cccttgttga
     1321 cgaccgcgta gctgatggcc ggcgtgggcg tatcggcccg cgccagccag gtgtggatgt
     1381 cgttagcggc catcaccctg gccacttcgg cggcgtaacg gtcggacagg aagcgcgtgt
     1441 cgaaaccgat gacaacactt ggcgcggccg ccccctggct gtggacgtag tcggcgatgg
     1501 cctgagcgac cagacgcagg ttatcgaagg tgaattcgtc gctgatgatg gctcgccagc
     1561 catcggtgcc gaatttgacg gtcatgagga ctccccagac tcttcgtcgg tgtcagtagc
     1621 gccgggaaca acgccgggct gacattggcc cagcgccgtt cagccggcat aggataggat
     1681 gacgacgagg tcattgacat tcgtgcccgt tgggccggtg accaccagat gggggtcaac
     1741 ctggccgcga gcagcctcgt cgagacggct gaaaaaagca tagctattgt tatcggccag
     1801 gtgggccagt gggtcgatgc cgtaggaacg ggccagcggc gcggtcgcgc cgctaacgat
     1861 cgcgcccgcg gccggcgtcg gcccgtcctc gccgtcggtg ctgaagctga gcacggccgc
     1921 gcgcggctgc cgctccagcg ccagcgccgc tgccagggcc aactcctggt tgcgcccgcc
     1981 gcgccccttg ccgcgcacgg tgacagtcgt ctcgccaccc atgatgtagc attggcccgg
     2041 cggcaggtcg cgggcaatgc cggccgccag ccggccgatg gcgcgggcct ccccgtccag
     2101 gtgggcagtc agcagctggc cgacaaagcc caggcgaatg gccgtcgcca gcgccgccgt
     2161 ggccgcctcg cgcacgtcgc cgacgatgaa gttgcgcacc gacggccggg ccgccttgtg
     2221 caggtagcgc gcctggttga gtgccaacgc cagccgctgc cactccgcct tctccaactg
     2281 gcgggctatg tcgtagcggc cgagcacggc caccgcgccg acaacgagat cgtcatccgg
     2341 cacggtcggc ccgccaccga tgaccggcag cgggttgccg acgacgtcgg acaggatcag
     2401 gccatagcac gaagccggcg cgcaggcccg tgccaggccg cccgccttga tccggtcgat
     2461 ggcgcggcgc acggcgttca gttcgctgat ggtgcaaccg ctcttgagga agacctcgac
     2521 cagcttgcgc caatcttcta gcggcagcag tggctgcgtc agcagggacg acgcgccgcc
     2581 tgaaatcagg cagaggacgg catcctcggc ccgtgttgcg gccagcaact ccaaaaccgc
     2641 tttcgtcccg gccaggctac ccgcttccgg caacggatgg ccgccccggt ggagggccag
     2701 gggccactgg ccggccacct ccggccactc gtggccgccg actttggcaa tgaccacgcc
     2761 gccggccacg tccgcgccca gcacggccat cgccgccgtg gtcatggaga tggccgcctt
     2821 gcccgcggcg accaggtaaa tgcggccacg atccaggtcg atggcctcag cttccgacgc
     2881 gcggccgacc cataacgtgc gcccctctcg ccgcaggaag cggctcacag cggcgttagg
     2941 gtcggccgcg gcgatggtcg ctccgacaag ttcgtccgca tggcggcggt agtcggcaaa
     3001 gcgaggcgag atggacgcag tggccgtcat ggtagttcga ggtgggcgca atcgttgagc
     3061 cgttccagac gggcgagtcc gtcgatgatg tccacctggc tgatggctgt atgattgcca
     3121 ccgttcaggc gcataaagcc gcttgggcca aagtccagca agtgcaccaa cgtcgccagg
     3181 atcatgcccc cgtggctgac gacggcgatt gtctgaccat cgtggcgggc gagcacggct
     3241 ttgcagccgg acacagcgcg gcgcagcacg tcgtcgggcg attcgccgcc cggcgcgtgc
     3301 atggagccgt ggtggcggct ggcccaggcg tcagggaagc gggtgatgat ctcatcggtc
     3361 gtcagccctt ccagttggcc aatggcacgt tcgcgccaga caggatcagt agtcattggc
     3421 cgccccagcg ccgcagcgat gatcgtcgcc gtgccttggg cgcgctgcaa atcgctggca
     3481 tagatgtggc tgatttcgat agccgtcgcg cggcagaggc gctcggccaa ccgttgggcc
     3541 tgggccaggc cgctctcgct cagtggcggg tcggcctgcc cttgccaacg gccggcttcg
     3601 ttccagacgc tacgcgcgtg gcggatgaac aggaggcgag taggcaatga cattgcagtg
     3661 acaggtgaca atgatggtca ggcgcggagg tcgtcactga gcgccgacag ggccgcgcca
     3721 tcgatcagcg tcatcggttt gtcggccgcc cattggcgtg cggcctcgga aatttcagcc
     3781 gttgtcacca ggaagccgtg gaaggcgcgc tcgtgcacca ttgtgccaaa cagctcgcgg
     3841 atgatgtcgg ggccaacctg gtggcgatag cgcttgcatt ggacgacggc gcggcggccg
     3901 tcagcccgcg tcagggccag gtcaacgccg aggtcgcccg cccggccgcg cacctcgacc
     3961 gtgtagccgc gccgctcgaa cagctcggcg acgaattgct cgaaagcgcg cgggctgagg
     4021 gcgtagagtt gctccacagt catgcgcgcc gttggctcca tgcgctgctg agcgcgccgc
     4081 tgccagacga tgagcgccca cagcagggtc agggtcagca ggctggcgcg cgtgaacagg
     4141 tccagcaggt cgcccagcca gtctggccag cgcgcggcgg cgttagcggc aatgccgtag
     4201 cgccatagaa cccacagcac aaagaaaccg gtggcggccg aaacgatcca ggtgaggcga
     4261 gcgagatgat cgcggctaat ggtatcctcg cgaatgatcg gtgtcgcgtt gggccggttg
     4321 gcggcattta gtcgtatcaa catcgggcgt ccgtggcgcg gccacatacg gtcgcaattc
     4381 caggcggtcg atcagtattg taatgccgcg gcgcgcaaag ccaaaattgg gacgaagggg
     4441 acaggggtta ggggttaggg gctaggggct agggaagagc gcgcggcgac cgcatgtcgg
     4501 cgcgtggcgt tcttccctgg aacctggaac ctggcccctt cctctgacaa atctccccgg
     4561 cagtgctata gtaccgtccg atgatcatca ccaagcgaca gttaggtggg ctgtttatcc
     4621 tgctgggcgt tggcgtggcc gtggggctgt tcgccatcga ctggctcggc gcggggcagt
     4681 tccagggcat cggcccagcg cagcggcgcg cgctgctgct ggccggcggc gtcgtgctgt
     4741 tggggctgtc gctgctgccc ctgggtaacc gcccggcatg acgcgctggc aaggtcgctc
     4801 ttgtcgctac acgaaattca cgatgcctgg gaagtcctcc tgaacaccga cgccacctcc
     4861 gccatgcccg tcgctcaggc cgccccgcgc acggccgccc tcctgcccag gctgctcatt
     4921 ggcctggctc tggccgcttt catcagctat ctggtcgttt acgtcatcta tgccgtgcag
     4981 ctcttgcgct tccccttcga ctacgatcaa ggcgaaggct tcgagctgat ggacacgatc
     5041 ctcttcagcc acggc
//
LOCUS       DAOSMQ010000220         5029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16809_length_5029_cov_5.428629, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000220 DAOSMQ010000000
VERSION     DAOSMQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16809_length_5029_cov_5.428629"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PK829_08420"
     CDS             complement(<1..571)
                     /locus_tag="PK829_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOU41277.1"
                     /translation="MSMRLRARAAGLLLPGLLALLVSAAGAQEAPAAASDEVAPKWTV
                     PLPNTTCSWSASEHNCHPSSPGVGDLNGDGRLDIVVATNNGHVVAVSNGAVLWDRDVA
                     GLFGMAANTQSFASSPAIADLDRDGALEVVVATSMNGTTCQPGGVIVLDSRGQPRPNW
                     PQLSDDDAGPIAHCPAPFISTPALGDLDGD"
     gene            complement(623..1861)
                     /locus_tag="PK829_08425"
     CDS             complement(623..1861)
                     /locus_tag="PK829_08425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU41278.1"
                     /translation="MSLPRRITLVVQRYGREVNGGAEQHARWLAERLAASAEVTVLTT
                     CALDYLTWADHYPAGESDIDGVRVCRYPVDAVRDWKRAQRQTRLVVNHHHTLFDESRW
                     VHDQGPFSTPLFEAIRRVYETTDAFIFYTFHYATTAFGLPIVSDKAFLVPTAHDDPFV
                     HMAVFRPLFHLPRGIFYLTEPERRLVQRVTRNGAVANTVTGVGVEPAVGASAARFREK
                     FGLAEPFLLYVGRVDESKNVPELLDFFTRYRQAAGTAPLKLVLIGKANMPLPNHPDIV
                     PLGFLPDADRDDAIAAATLVVLPSLYESLSMITLEAWAGGVPVLVNEQCEVVKYLCRQ
                     SNGGLYYRTHDEFTAALRLLLGSEALRWQMGLQGQQFVLRHYRPEVVLRHYTTLLDAA
                     VRARPSSARARQGHEEEAAQ"
     gene            complement(1858..3009)
                     /locus_tag="PK829_08430"
     CDS             complement(1858..3009)
                     /locus_tag="PK829_08430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU41279.1"
                     /translation="MTTTQSTSQPNADSSFIPPPSSFGALRVHIMTAGLSPGDAVSNY
                     ALNLARLFRQWGARATVYADHIAPALQGRAEPSRFYRPTGEDLLWFHYSIWADNVELA
                     LASPDFKIMDYHGICPPRLFAGQNAHLASLCQHGLDVLPTLAGRFEQVVIHSEDSRRV
                     LQENGFPADRLHKIFYTTDTAKFTAAPDDALAALLARLDYVLLVGRIVPQKDVGALID
                     IFARLHAARPGLVLILAGTRDQTKAYQAELDRQIAAYGLENRVLFAGQVNNPAVLGAL
                     YQNARLLFVTSEWESFCVPIAESLYFDVPVAVHDQEPMVEVAGPAGLVFDKRRPDEAA
                     AQVLALLDDPARYAAMRAEAGRWAQRYNDAALEANVRRFLMTTFALTPA"
     gene            complement(3006..4199)
                     /locus_tag="PK829_08435"
     CDS             complement(3006..4199)
                     /locus_tag="PK829_08435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU41280.1"
                     /translation="MTTISFVTPWYGPDVPGGAEAELRRLAPQLQRAGMAVEVLTTTT
                     RDLYADWGRSYHPAGPAVIDGVPVRRFPVQRRNRAAFDALNLRLMNNRPISAADERVF
                     IEEMLRVPALYDFIAAEASERLYYFIPYLFSTTYFGAQVCPGRSIIMPCLHDESYARL
                     GLYREVLSRARALVFHTEAERALADQLFPAQSGQLREVFGAGVDVEGEGDAARFRRRT
                     GVDGPFVLYAGRREPGKNTPLLLDYWARYWAATGRRRDARLVLLGSGEVALPPAAAGG
                     ILDLGFVPPQDKLDALAAATVFCLPSVNESFSIALMESWLQGTPALVHAGCAVTVEHC
                     HNANGGLYFGDYDEFAATLDYLFDHPAEAAQMGRNGRAYVLSRYQWPIIIPRYSDLFA
                     RVLAP"
     gene            complement(4209..4607)
                     /locus_tag="PK829_08440"
     CDS             complement(4209..4607)
                     /locus_tag="PK829_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41281.1"
                     /translation="MSEQPATEPQVNIEEIMQQIRQQIIAKRAAVGVGAGGSAGAAPI
                     VTGQRYPPEFYEHLYQARLALDEVEVPVFVSKSGVPLIGGLLVWLRTKLHELVAYYVN
                     LSAARQASAAEHLLQALSLVGQEDQAGDGR"
     gene            complement(4664..>5029)
                     /locus_tag="PK829_08445"
     CDS             complement(4664..>5029)
                     /locus_tag="PK829_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41282.1"
                     /translation="ALNAALEPDAVLLFYDGVPIGNGDFFGTPLQFIYGHHAFALRDP
                     ALPGADLVRAVEIWHNSGRAVYWIGDPAWLGEHGLAYEEATYTLRSQRLEASYVHRPT
                     AILTDEWVLRVARVAPDKE"
BASE COUNT          880 a         1612 c         1766 g          771 t
ORIGIN      
        1 cgtcgccgtc caggtcgccc agcgccgggg tgctgatgaa gggggcaggg cagtgagcga
       61 tggggccggc gtcgtcgtca ctgagttggg gccagttggg gcgcggctgt ccgcggctgt
      121 cgaggacgat cacgccgccc ggctggcaag tcgtgccgtt catgctggtg gcgacgacga
      181 cttcgagcgc gccatcacgg tccaggtcgg cgatggccgg cgaagaggcg aagctctgcg
      241 tgttggcggc cataccaaac agaccggcca catccctgtc ccacagaacc gcgccgttgg
      301 acacggccac aacgtggccg ttgttggtcg ccacaacgat gtcgagacgg ccgtcgccgt
      361 tcaggtcgcc cacgccgggc gaactgggat gacaattgtg ctcggaggcc gaccaggagc
      421 aagtggtgtt gggcaggggc acagtccact tcggcgcgac ctcgtcgctg gccgcggccg
      481 gagcctcttg ggcaccagcg gcagacacga gtaatgccag aaggcccggc agcagcagcc
      541 cggccgctct ggcgcgtaga cgcatagaca tgacaactcc ttggctatga cacggccggg
      601 agcatctggg gccactcaaa gctcattggg cggcttcctc ctcatggccc tggcgcgcgc
      661 gggcgctgga cgggcgagca cgaacggcag catcaagcag agtggtatag tgtcgcagca
      721 ctacctcagg gcggtagtgg cgcaggacga actgctgtcc ctgaagaccc atttgccacc
      781 gcagggcctc ggagccaagc aggaggcgga gggcggcagt gaattcgtca tgcgtgcgat
      841 agtatagtcc gccgttgctc tgccggcaca ggtatttgac cacctcacat tgttcgttca
      901 cgagcaccgg cacgcccccc gcccaggcct ccagggtaat catcgacagg ctctcataga
      961 gcgagggcag caccaccagc gtcgccgcgg cgatggcatc gtcccggtcg gcgtcgggca
     1021 ggaagcccag aggaacgatg tcggggtggt tgggtagggg catgttggcc ttgccgatga
     1081 gcaccagctt gagcggcgcg gtgccggccg cctgccggta gcgagtgaaa aagtccagca
     1141 gctccggcac gtttttggac tcatcgacgc ggccgacata gagcagaaat ggctccgcca
     1201 ggccaaactt ctcgcggaag cgcgccgccg aggcccccac ggccggctcc acgcccacac
     1261 ccgtgaccgt gttggccaca gcgccattgc gcgtcacgcg ctgcaccagc cgccgctccg
     1321 gctcggtcag gtagaagatg ccccgcggca ggtggaacag cggccggaag acggccatgt
     1381 ggacgaaggg atcatcgtga gccgtgggca ccagaaaggc cttgtcggag acaatgggca
     1441 gcccgaaggc ggtggtggcg tagtgaaacg tgtagaagat gaaggcgtcg gtcgtctcgt
     1501 agacgcggcg gatggcctca aagaggggcg tggagaacgg cccctggtca tgaacccagc
     1561 ggctttcgtc gaacagggtg tggtggtggt tgacgaccag gcgcgtctgc cgctgggcgc
     1621 gcttccaatc gcgcacggca tcgaccgggt agcggcacac gcgcacgccg tcgatgtcgc
     1681 tctcccccgc cgggtagtgg tcggcccagg tgaggtagtc gagggcgcag gtcgtcagca
     1741 cagtgacctc ggctgaggcc gccaggcgct cggccagcca gcgggcgtgc tgctccgcgc
     1801 cgccattgac ctcgcggcca tagcgctgca cgacaagggt gatgcgacgg ggtaggctca
     1861 tgcgggcgtc agggcgaacg tcgtcatcag gaagcgccgg acgttggcct ccagcgcggc
     1921 gtcgttgtag cgctgcgccc agcgcccggc ctcggcgcgc atggccgcgt agcgggccgg
     1981 gtcgtcgagc agggccagca cctgggcagc ggcctcgtcg ggccgccgct tgtcgaagac
     2041 cagcccggcc ggcccggcca cctcgaccat cggctcctgg tcgtggacgg ccacgggcac
     2101 gtcgaagtag agcgactcgg caatgggcac gcagaagctc tcccactccg acgtgacgaa
     2161 cagcagccgg gcgttctggt agagcgcccc caggacggcc gggttgttga cctgtccggc
     2221 gaagaggacg cggttctcca ggccataggc ggcgatctgg cggtcgagtt cggcctggta
     2281 cgcctttgtc tggtcgcgcg tgccggccag gatgagcacc aggcccggcc gcgcggcgtg
     2341 cagccgggcg aagatgtcga tcagcgcgcc gacgtccttc tgcggcacaa tgcgcccaac
     2401 gaggagaacg taatccagcc tggccagcag cgcggccagc gcgtcgtccg gcgcggccgt
     2461 gaacttggcc gtatcggtcg tgtagaaaat cttgtgcagc cggtcggcgg ggaagccgtt
     2521 ttcctgcaag acgcgccgcg agtcttcgga gtggatgacg acctgctcga agcggccggc
     2581 cagcgtcggc agcacgtcca gcccatgctg gcagagggac gccaggtgcg cgttctgccc
     2641 ggcaaagagg cgcggcgggc agatgccgtg gtagtccatg atcttgaagt cggggctggc
     2701 cagggccaac tcgacgttat cggcccagat ggagtaatgg aaccacagca agtcttcgcc
     2761 ggtcggccgg tagaagcgcg acggttcggc ccgcccttgc agcgccgggg cgatgtggtc
     2821 ggcgtagacc gtcgcccgcg cgccccactg ccggaacagg cgggccagat tgagggcgta
     2881 gttgctgacg gcgtcgccgg gcgacaggcc agcggtcatg atgtggacgc ggagagcgcc
     2941 gaaggatgag ggcggaggga tgaaggatga gtcggcgttg ggctgggagg tcgattgggt
     3001 tgtagtcatg gggccaggac gcgcgcgaac aggtcgctgt agcgtgggat gatgatcggc
     3061 cactgatagc gcgacaggac ataggcgcgg ccgttccgcc ccatttgcgc cgcctcggcc
     3121 ggatggtcaa aaagatagtc aagggtcgcc gcgaactcgt cgtagtcgcc aaagtagagg
     3181 ccgccgttgg cattgtggca atgctccacc gtcacggcgc agccggcatg aaccagcgcc
     3241 ggcgtgcctt gtagccaact ctccatcagg gcgatagaga agctctcgtt gaccgacggc
     3301 agacaaaaga ccgtcgccgc ggccagggca tcgagcttgt cttgcggggg gacgaagccg
     3361 aggtcgagaa tgccgccggc agccgcgggc ggcaaagcca cttcgcccga ccccaggagc
     3421 accaggcgcg cgtcgcgccg ccttccggtc gccgcccagt agcgcgccca gtagtcgagc
     3481 agcaggggcg tgttcttgcc cggctcgcgc cgcccggcat agaggacgaa ggggccgtcc
     3541 acaccggtgc ggcggcggaa acgcgcggcg tcgccttcgc cctctacgtc tacgcccgcg
     3601 ccgaatacct cgcgaagttg ccccgactga gcggggaaca gttggtcggc cagggcgcgc
     3661 tccgcctccg tgtggaagac aagcgcccgc gcccgcgaca acacttcgcg atagaggccc
     3721 agtcgcgcgt aactctcgtc gtggagacaa ggcatgatga tgctgcggcc ggggcagacc
     3781 tgcgctccga aataggttgt ggagaacagg tagggaatga agtaatacag ccgctcggac
     3841 gcctcagcgg cgatgaagtc atagagcgcc ggcacgcgca gcatttcttc gataaagacg
     3901 cgctcgtcgg cggcggagat gggccggttg ttcatcaggc gcaggttaag cgcgtcaaaa
     3961 gcggccctgt tgcgccgctg caccgggaag cgccgcacgg gcacgccgtc aatgaccgcc
     4021 ggcccggcgg gatggtagct gcgcccccag tcggcgtaga ggtcgcgcgt cgtcgtcgtc
     4081 aacacctcga cggccatgcc ggcgcgctgc aactgcgggg ccagccgccg cagttccgcc
     4141 tcggctccgc cgggcacgtc tggcccgtac cagggtgtga caaaggaaat agtggtcatg
     4201 ggcgcggcct agcgtccgtc gccggcctgg tcctcctgac cgaccagact gagggcttgc
     4261 agcaggtgct cggcagcact ggcctgccgc gccgcgctca agttaacata ataagcaacc
     4321 agttcatgga gcttcgtccg cagccagacc aacaagccgc caataagggg cacgcctgat
     4381 ttgctgacaa agacgggcac ttcgacctcg tccagcgcca gccgcgcctg atagaggtgc
     4441 tcgtaaaact ccggcggata gcgctggccg gtcacaatcg gcgctgcgcc ggcgctgccg
     4501 ccggccccca cgccgacggc cgcccgcttg gcgatgatct gctgccggat ctgctgcatg
     4561 atctcttcga tgttgacttg cggttccgtt gcaggctgct cactcacgtc gatatcaact
     4621 ccttaggcct catcagggcg gccgaacgcc ccccgttgtg ttcttactct ttgtctgggg
     4681 caacacgcgc cacgcgcaac acccactcat cggtcaggat cgccgtcggc cggtggacgt
     4741 agctcgcttc cagtcgttga ctgcgaaggg tgtaggtcgc ctcttcgtag gccaagccgt
     4801 gttcgcccag ccaggccggg tcgccaatcc agtagacagc gcgaccgcta ttgtgccata
     4861 tctccacggc ccgaaccaag tccgcaccgg gcagcgccgg gtcgcgcagg gcaaacgcat
     4921 ggtggccata gatgaattgc agcggcgtgc cgaagaagtc gccgttgcca atgggcacgc
     4981 catcgtagaa cagcagcacc gcgtccggct ccagcgcggc gttgagcgc
//
LOCUS       DAOSMQ010000221         5002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16921_length_5002_cov_4.943602, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000221 DAOSMQ010000000
VERSION     DAOSMQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16921_length_5002_cov_4.943602"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PK829_08450"
     CDS             <1..465
                     /locus_tag="PK829_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41283.1"
                     /translation="VSWGRATALRDKVAAAEEAIYADTRDPLVNWDADDLRAALAAAG
                     LVDVRLTVEEQTEERRITAGHLDRWFPALSPSLREGLGEGGTEGPLPQGEGTGARLSY
                     GERLLAGGLSAAEVGKVAKLLREQLTEQTVGWRSRLAFVRAERGAILPNRAE"
     gene            823..1560
                     /gene="rph"
                     /locus_tag="PK829_08455"
     CDS             823..1560
                     /gene="rph"
                     /locus_tag="PK829_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOU41284.1"
                     /translation="MECLARARRDGRGPADLRPIAFDTGFTRWAEGSVLARFGETHVL
                     CNVTIENSLPPWLKNRMPPQGWLTAEYAMLPRSTHTRSPREQRWPKGRTQEIGRLVGR
                     SLRAALDLSLLGERTLTVDCDVLQADGGTRTAAISGGWVAVALALRPLIAAGALPAAV
                     LRRQIAAVSVGIVAGEALLDLDYSEDSAAGVDLNVVMTATGDFVEVQGTAEGEPFPRG
                     QLDALLARATAGVAALCAAQTRALADA"
     gene            1621..1896
                     /locus_tag="PK829_08460"
     CDS             1621..1896
                     /locus_tag="PK829_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOU41285.1"
                     /translation="MEDIHWNSEKDQQLRESRGISFESIVPYILQGDILDIYDHPNQE
                     RYPGQQFFVVQTDTYVYLVPFIEKDGQVFLKSIIPSRKAKRKYSKRE"
     gene            1898..2236
                     /locus_tag="PK829_08465"
     CDS             1898..2236
                     /locus_tag="PK829_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41286.1"
                     /translation="MDDFELDEEEREILEAFESGQLTPIANKDEVIARLQSYAQSTQR
                     KDRRVNIRLTANDLAALQSIAVDEGIPYQTLMASILHKYVTGRLVERPRRPQPAADYA
                     VRESGEPYNE"
     gene            2327..3568
                     /locus_tag="PK829_08470"
     CDS             2327..3568
                     /locus_tag="PK829_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOU41287.1"
                     /translation="MATIDFRHEAEGLFDEMVRVRRDLHRHPELGFQETRTAGIVATT
                     LNELGLEVRAGVGQTGVVGLLEGDRPGPTVLLRFDMDALPIQEESDHGYVSLNPGVMH
                     ACGHDGHVAMGLALARLFAARRAELGGAIKFLFQPAEEGLGGALAVIADGALEEPRPD
                     VALAMHLWSPVSLGQARVVDGPAMAASSVFTITVEGKGGHGAAPHLCTDPILAAAQIV
                     VALQSIVSRNTDPHESVVLSIGAFSAGTTFNVIPERAVLKGTVRSYDTPSHRRVYRRL
                     LEMATHTASAYGCRATMETVAIVAAVVNDPEPTAVVRASAGRVLGEGNILEHRTMAAE
                     DMGFVLQEIPGCYFFIGCSDGNLTSYPHHHPRFDFDERAMIDGVAVMAGAAAHYLLND
                     DGRAAVDEPGAWVAADRANGR"
     gene            complement(3666..4133)
                     /locus_tag="PK829_08475"
     CDS             complement(3666..4133)
                     /locus_tag="PK829_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41288.1"
                     /translation="MRKLLQNDQGHWNPQIIAALIAAGATLLTAIIAGVFGLLQLRAA
                     NGPAVAPATAPTAALTVEIDGPAEAPLNQVTAFTLISENAVRAEWAIAGFGNDDINPF
                     HLSEQVEVEPTNAERVGDSFTLVVTVYDAAGNRANARHVFAIAPADIIVAPEE"
     gene            complement(4400..4741)
                     /locus_tag="PK829_08480"
     CDS             complement(4400..4741)
                     /locus_tag="PK829_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOU41289.1"
                     /translation="MSDQINVVEAKSRFAEWMNRVAYQGQRVVIERHGKPMMAWISMD
                     DLARLETIEAPHNILQQRLAALDMANEVRAQIRAERLGQPVPDSTETLHALREGQELV
                     TGRAVQSSNSL"
BASE COUNT          846 a         1563 c         1729 g          864 t
ORIGIN      
        1 gtcagttggg gccgggcgac ggcgttgcgc gacaaggtgg ccgccgccga agaggccatc
       61 tacgccgaca cccgcgatcc actggtcaac tgggacgccg acgacctgcg cgcggcgctg
      121 gcggcggccg gactggtgga cgtgcggctg acggtggagg agcagacgga ggagcggcgg
      181 attacggcgg ggcatctgga tcgttggttc ccggctttgt ccccctccct ccgggagggg
      241 ttaggggagg gtgggacgga aggccctctc ccacagggag aggggaccgg ggcgcggttg
      301 agctatggcg agcggttgct ggcgggcggg ttgtcggcgg ctgaggtagg gaaggtggcg
      361 aaactgttgc gcgagcagtt gacggagcag acggtcggct ggcgttcgcg gctggcgttt
      421 gtgcgcgctg agcgtggcgc gattctcccg aatcgcgcgg agtgagaaga gcgattcgga
      481 gaatcgcgcc acgatgaaag aggtctgatg atgacttatc gagcgaccaa gccttgggcc
      541 gttcactacc ctgatcctgt tggcggccgg gccggggata ccttcgctct tggccggcgc
      601 gacgatgagt actctggctg ggtctgggcc acggccgaag atggccgcga gggctggatt
      661 cccgaaagct ggctgtgcgt ggagggggac agcggcgtgc tggtgcgcga ctacaccgcc
      721 gccgagttgg ctctggagcc gggcgacgtg gttagcggcg acctggtgga gagcggttgg
      781 ctgtgggcca cggtggccga tggccggtcg ggctgggcgc cgctggagtg cctggcccgc
      841 gcgcggcgcg acgggcgcgg cccggccgac ctgcgtccca tcgccttcga cacgggcttc
      901 acgcgctggg ccgagggttc cgtgctggcc cgcttcggcg agacccacgt cctctgcaac
      961 gtcaccatcg agaacagcct gccgccctgg ctgaagaacc gcatgccgcc gcagggctgg
     1021 ctgacggccg agtatgccat gctgccgcgc agcacccaca cgcgcagccc gcgcgagcag
     1081 cgttggccca aggggcgcac gcaggagatc ggccggttgg tcggccgcag cctgcgcgcc
     1141 gccctcgacc tgtcgctgct gggcgagcgg acgctgacag tcgattgcga tgtgttgcag
     1201 gccgatggcg gcacgcgcac ggcggccatc agcggcggct gggtggcggt ggccctggcc
     1261 ttgcgcccgc tgatcgccgc cggcgcgctg cccgcggccg tcctacggcg gcagatcgcc
     1321 gccgtcagcg tgggcatcgt cgctggcgag gcgctgctcg atctggacta cagtgaggac
     1381 agcgccgcgg gcgtcgatct caacgtcgtc atgaccgcga cgggcgactt cgtcgaggtg
     1441 cagggcacgg ccgagggcga gccattcccg cgcggccagc tcgacgcgct gctggcgcgg
     1501 gccacggccg gcgtcgccgc gctgtgcgcg gcacagacgc gcgcgttggc cgatgcctag
     1561 atgagttgca ctgtagggcg acctgttgcc gttcggcaca cgttgtgata cgctatgaga
     1621 attgaggata ttcactggaa cagcgaaaaa gatcagcaat tgcgtgagag tcgcggcatt
     1681 tcttttgaga gcatcgtgcc ctacatcttg caaggcgaca tcctagacat ctatgaccac
     1741 cccaatcagg aacgataccc gggacaacag tttttcgttg tccagactga cacctatgtt
     1801 taccttgtac cattcattga aaaggatgga caagtctttt taaagtcgat cattccgagc
     1861 cgcaaggcga agcgaaagta ttctaagcgg gagtaagatg gacgacttcg agttggacga
     1921 agaagaacgg gaaatactgg aagcctttga gagcggtcaa ttgaccccta tcgccaataa
     1981 ggacgaggtc atcgcccgcc ttcagtccta tgcccagagc acccagcgca aagaccgccg
     2041 cgtcaacatc cgcctgaccg ccaacgacct ggctgccttg cagtctatcg ccgtggacga
     2101 gggcatcccc taccagacgc tcatggccag catcttgcac aagtacgtca ccgggcggct
     2161 ggtcgaacgg ccgcgtcgcc cccagccggc ggcggactac gccgtgcgcg aaagcggcga
     2221 gccatacaac gagtagccct gccccccagg acgctgtaat gcgcctagta ataccaatct
     2281 attagactgt ctttgtaaca aattcctcga tgggaagctc tgattcatgg caacgatcga
     2341 tttcaggcac gaggccgagg gcctcttcga cgagatggtc cgcgtccggc gcgatttgca
     2401 tcgccacccg gaactcggct tccaggagac gcgcacggcc ggcattgtcg ccacgacgct
     2461 gaatgagctg gggttggagg tgcgcgcggg tgtaggccag acgggcgtcg tcggcctgct
     2521 ggagggcgac cggcccggcc cgaccgttct gctgcgcttc gatatggacg cgctgcccat
     2581 tcaggaggag agcgaccacg gctacgtgtc gctcaatccc ggcgtcatgc acgcctgcgg
     2641 ccacgacggc cacgtggcta tgggcctggc cctggcgcgg ctctttgccg cgcggcgggc
     2701 ggagttgggt ggggcgatca agttcctctt ccaaccggcc gaggaagggc tgggcggggc
     2761 gctggcggtc atcgccgacg gggcattgga ggagccgcgg ccggacgtgg ccctggccat
     2821 gcacctgtgg tcgccggtgt cgctggggca ggcccgcgtg gtcgatggcc cggcgatggc
     2881 cgcgtcgagc gtcttcacca tcactgtcga gggcaagggc ggccacgggg ccgcgccgca
     2941 cctgtgtacc gaccccattc tggccgcggc gcagatcgtc gtggccttgc agagtatcgt
     3001 cagccgcaac accgacccgc acgaaagcgt cgtcctgtcc atcggcgcgt tctcggcggg
     3061 cacaaccttc aacgtcatcc cggagcgggc ggtgctgaag ggcacggtgc gcagctacga
     3121 cacgccgtcg caccgccgcg tctaccgccg cctgctggag atggccacgc acacggccag
     3181 cgcctacggc tgccgggcga cgatggagac ggtggccatt gtcgccgcgg tggtcaacga
     3241 cccggagccg acggccgttg tccgcgcctc tgccgggcgc gtgctgggcg agggcaacat
     3301 cctggaacac cgcacgatgg ccgccgagga tatgggcttt gtcttgcagg agatccccgg
     3361 ctgctacttt ttcatcggtt gcagcgacgg caatctgacc agctatcccc accaccaccc
     3421 gcgtttcgac ttcgacgagc gggcgatgat cgacggcgtg gccgtgatgg ccggggccgc
     3481 ggctcactat ctgctcaacg acgacggccg ggccgctgtg gacgaacccg gcgcatgggt
     3541 ggccgccgat cgggcgaacg ggcgctaaag ctaaaccatt ccagattcac gcgcaaccgt
     3601 ccgcccgatc tgcggcgatc tgtgtgctga aggagcggcg cgccggcaac ggcgcgccgc
     3661 tcgtgctatt cctcgggcgc gacgatgatg tccgccgggg caatcgcgaa cacgtggcgg
     3721 gcgttggcac ggttgccggc ggcgtcgtag acggtgacga ccagggtgaa cgaatcgccc
     3781 acgcgctcgg cgttggtcgg ctcgacctcg acctgttcgc tgaggtggaa ggggttgatg
     3841 tcgtcgttgc cgaagccggc gatggcccac tcggcgcgga cggcgttctc gctgatgagg
     3901 gtaaaggccg tgacctggtt gaggggggcc tcggccgggc cgtcgatctc gacggtcagg
     3961 gcggccgtgg gggcggtggc gggcgcgacg gccgggccgt tggcggcgcg cagctggagc
     4021 aggccgaaga cgccggcgat gatggccgtc agcagcgtcg ccccggcggc gatgagggcg
     4081 gcgatgatct gggggttcca gtggccttgg tcgttctgga gcagtttgcg cataggcgca
     4141 ccacctatcc catcgtgtcg gacggtagtg tagccgaatg gcttagaaga aggcaaccac
     4201 ccgcgggata ctatctgtgc tgttggccct ggtctatttc agcaggtatg gttacggccg
     4261 gttgagagga tagaggctgt gccagactca ctccaatagc cttagccagg gcagagcaaa
     4321 tcccgcggcg ttatagagcc ggcggtcggc tgtccatact tcacagccca ataactcgcc
     4381 caacgccagg taagtcgcgt catagactgt tggacgattg aaccgcgcgg ccagtaacca
     4441 gttcctgccc ctctcgcaga gcatgcagcg tttcggtgga gtcgggcaca ggctgaccaa
     4501 gtcgctccgc gcgaatctga gcccgaacct cgttggccat gtccagcgca gccagacgct
     4561 gttggaggat gttatgtggc gcttctatgg tctccaaccg ggccaggtca tccatactaa
     4621 tccaggccat catcggtttc ccatgccgct cgatgaccac tcgctgcccc tgataggcaa
     4681 cacgattcat ccattcggcg aagcgtgact tggcctcgac cacgttaatc tggtcactca
     4741 ttccacacct ttgggtcata atgaactata tgacctgata ttagctcttg aaggcgctat
     4801 cggcaatcgc atgaggtcat accttttagc acgcgcacct gccgcccatc tcaaatgtca
     4861 actgccaggg catcactcgt ccagcgtcaa cacatcctcc accacccagt acggcccggg
     4921 gcacgtgtcg gggatgctgt ccagccgtaa gacctcggcg tccagcagcg gctcgatctc
     4981 cagcagccgg ccgccactca gc
//
LOCUS       DAOSMQ010000222         5000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_16933_length_5000_cov_4.431749, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000222 DAOSMQ010000000
VERSION     DAOSMQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16933_length_5000_cov_4.431749"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2826)
                     /gene="glyS"
                     /locus_tag="PK829_08485"
     CDS             complement(<1..2826)
                     /gene="glyS"
                     /locus_tag="PK829_08485"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOU41290.1"
                     /translation="MTTPLNFQQMILRLLDYWQAQGCLIWQPYNVQVGAGTMNPATIL
                     RVLGPEPWNVAYIEPSIRPDDGRFGENPNRMQQHYQLQVILKPDPGNPIELYLNSLAA
                     IGISQREHDIRFVEDNWQSPALGAWGLGWEVWLDGQEITQYTYFQQAGGLDLNPVSVE
                     ITYGLDRIALALQGKNSVWEMAYGAGVGYGDALLQAEIEHCKYYFDVADVDALKNIYD
                     TYEREALRCLDAGLVAPAHDYNLKCSQLFNVLDTRGAIGVTERAAYFGRMRRVANKVS
                     TAYVAQRQQMEYPLAEKGARGQGPGAREENTSSETRNPEPGTHPPADGDTFVLEIGSE
                     ELPHGDLTSALEQLRAAVPALLDAARLAHGDIRVQGTPRRLAVIVEGLAARSTDVEST
                     VKGPPADRAFDANGRPTQAAAGFARSRGLSVDDLTVVEEGGKRYVSAVVREAGRPSLE
                     ALAERLPDLIAGIKFEKSMRWNATNVSYSRPLRWLLALHGPAVVPFRYAGADSGRVTY
                     GLRPFGSPVISVADADAYLPALAEATIVLDMDDRRAAIAAGAEALARSVHGSIPADPA
                     LLDEVTNLVERPTPLLGSFEERFLALPPMALVAVMRKHQRYFPVYKGERLLPHFVAVR
                     NGDAEHLDIVRDGNEHVIRARFADAAFFYDKDIKRGLAEFVPELGKLTFQTGLGSMLD
                     KTRRLEQLVPTVAGMLGLSDAERATASRAAALAKADLATSMVIEMTSLQGKMGGHYAL
                     RGGEPAEVATAIAEQYDAVSSTRPALALALADRLDSLAGLFAAGLGPKGSNDPFGLRR
                     AALQLIENLTAHGQRFDLRAGLDAAGALLPVAWSDAGRAEVMAFIAGRLEGVLRDAGH
                     PAGIVKAVVAENAHDPNAARQAAAALAEAVRLSDWPALLNAYARCVRITRPLPQRYTL
                     RPDDLALPEERAVLDALLAAEVEKDGSV"
     gene            complement(2991..3770)
                     /gene="recO"
                     /locus_tag="PK829_08490"
     CDS             complement(2991..3770)
                     /gene="recO"
                     /locus_tag="PK829_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOU41291.1"
                     /translation="MPRERTYRTEAIVVRRSDFGEADRLLTLFSPERGKMRAIAKGAR
                     KPQSRKTGHVELFMRSKFLLAEGRDLDIVTQAETVDTYAPLRESLVRATYANYAVELL
                     DRFTVDEDPHRNIYELLTLALETLAAADQPLLLARFYELRLLSLTGFQPRLFNCVACG
                     EAIAEQDQFFSPELGGLLCPACGPTDPRAMPVSAVAVKVMRYLQTRPWATVSQLRLQP
                     AVLHEVERVLYDYLTHILERELKSVDFLHRLRREAELLAEE"
     gene            3920..4234
                     /locus_tag="PK829_08495"
     CDS             3920..4234
                     /locus_tag="PK829_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41292.1"
                     /translation="MFLDSALAPTPTPAPAVWRAVAIPLPPLPDVGDAALVTPEEAED
                     QPPAQLFGHFAEGTPVELLPPQAAPQVFLTPPRNADYLRLIVVKDSNYVGEPHQIQIV
                     VE"
     gene            4365..>5000
                     /locus_tag="PK829_08500"
     CDS             4365..>5000
                     /locus_tag="PK829_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41293.1"
                     /translation="MPTPQQSLLDHATDSLSCRANLRWLIGGSASGKSTIARTLGART
                     GIAVYDMDEAVFGRFHFDPIRHPATTAWFTAANPLAWMLSRPWAEFEALYRANNAEML
                     DLLAGDLAGRPDAPLLIDGGITHPSVLTQVIPAGRIVCLETDDARRRHEWATAPGRAG
                     MRAEILALPDGAALWRRFLDYDQRLTDTIGRESRDAGIRVLAWGEGIGVEDV"
BASE COUNT          758 a         1668 c         1770 g          804 t
ORIGIN      
        1 cacgctgcca tccttctcca cctcggcggc cagcaaggcg tccagcacgg cgcgctcctc
       61 gggcaaagcc aggtcgtcgg gccgcaaggt gtagcgctgc ggcagcggcc gggtgatgcg
      121 cacgcagcgg gcgtaggcgt tgagcagcgc cggccagtcg gacaagcgca ccgcctcggc
      181 cagcgccgcg gccgcctgcc gcgccgcgtt ggggtcgtgg gcgttctcgg cgacgacggc
      241 cttgacgatg ccggccgggt ggcccgcgtc gcgcagcacg ccctccagcc gcccggcaat
      301 gaaggccatc acctcggcgc ggccagcgtc gctccaggcc acgggcagca gcgccccggc
      361 ggcatctagc ccggcacgca ggtcgaagcg ctgcccgtgg gcggtcaggt tctcgatcag
      421 ttgcagcgcg gcgcggcgca ggccgaaggg gtcgttggag cctttcggcc ccagcccggc
      481 ggcaaacagg ccggccaggc tatccagccg gtcggccagg gccagggcca gcgccgggcg
      541 cgtgctgctg acggcgtcgt actgctcggc gatggccgtg gccacctcgg ccggctcgcc
      601 gccacgcagg gcgtagtggc cgcccatctt gccttgcagg ctggtcatct cgatgaccat
      661 gctcgtggcc aggtcggcct tggccaacgc cgcggcgcgg ctggctgtgg cccgctcggc
      721 gtcgctcagg cccagcatcc cggccacggt gggcacgagt tgctccaagc gccgtgtctt
      781 gtccagcatc gagccgagac cggtctggaa agtcagcttg cccaattccg gcacaaactc
      841 ggccaggccg cgcttgatat ccttgtcgta gaagaaagcc gcgtcggcga agcgggcgcg
      901 gatgacgtgc tcgttgccgt cgcgaacgat gtcgaggtgc tcggcgtcgc cgttgcggac
      961 ggcgacgaaa tggggcagca gccgctcgcc cttgtaaact ggaaagtagc gctggtgctt
     1021 gcgcatgaca gccactagcg ccatcggcgg cagggccagg aaacgctcct cgaaagagcc
     1081 aagcaggggc gtcggccgct ccaccaggtt ggtcacctcg tccagcaggg cggggtcggc
     1141 cgggatgctg ccgtggacgg agcgggccag cgcctctgcg ccggcggcga tggccgcgcg
     1201 gcggtcgtcc atatccagga caatggtcgc ctcggccagg gcgggcaggt aggcgtcggc
     1261 gtcggccacg gagatgaccg gcgaaccgaa ggggcgcagg ccgtaggtca cgcgaccgct
     1321 atccgccccg gcgtagcgga agggcacgac cgccgggccg tgcagggcca gcagccagcg
     1381 cagcggccgg ctatagctga cgttggtggc gttccagcgc atcgacttct caaacttgat
     1441 cccggcgatg aggtcgggca ggcgctcggc cagcgcctcc agcgacggcc gccccgcctc
     1501 gcgcacgacg gcggagacgt agcgcttgcc gccctcctcg acgacggtca ggtcatcgac
     1561 cgacaggccg cggctgcggg cgaagccggc ggcagcctgg gtgggccggc cgttggcgtc
     1621 gaaggcgcgg tcggccggcg gccctttgac ggtcgattcg acgtcggtcg agcgcgccgc
     1681 cagcccttcg acgatcaccg ccagccggcg cggcgtgccc tggacgcgga tgtccccgtg
     1741 ggccagccgg gcggcgtcga gcagcgccgg cacggcggcg cgcaattgct ccagagcgct
     1801 ggtcaggtcg ccgtggggca gttcttcgct gccgatctcc aggacgaagg tatcgccgtc
     1861 ggccggggga tgggttccag gttccgggtt ccgggtttcg gaggaggtgt tctcctccct
     1921 ggcccctggc ccctggcccc tggccccttt ttcggccaac gggtactcca tctgctgccg
     1981 ttgggcgacg taggcggtgg agaccttgtt ggccacgcgg cgcatgcggc cgaagtaggc
     2041 cgcccgctcg gtgacgccga tggccccccg cgtgtccagc acgttgaaca gttgcgagca
     2101 cttcaggttg tagtcgtggg ccggggccac cagaccggcg tcaaggcagc gcagggcctc
     2161 gcgctcgtag gtgtcgtaga tgttcttcag cgcgtccacg tcggccacgt cgaagtagta
     2221 cttgcaatgc tcgatctcgg cttgcagcag cgcgtcgcca tagcccacgc ccgcgccata
     2281 ggccatctcc cagacgctgt tcttgccctg cagcgccagg gcgatgcggt cgaggccgta
     2341 ggtgatctct accgagacgg ggttcaggtc cagcccgccg gcttgctgga agtaggtgta
     2401 ctgggtgatc tcctgcccgt cgagccagac ctcccagccc aggccccacg cgccgagggc
     2461 gggcgactgc cagttgtcct caacgaagcg gatgtcgtgt tcgcgctggc tgatgccgat
     2521 ggccgccagg ctgttcaggt acagctcgat ggggttgccg gggtcgggct tgaggatgac
     2581 ttgcaactgg tagtgctgct gcatgcggtt ggggttctcg ccgaagcggc cgtcgtccgg
     2641 gcggatgctc ggctcgatgt aggccacgtt ccacggctcc gggccgagga cgcgcaggat
     2701 ggtggccggg ttcatcgtgc ccgcgccgac ctgcacgttg tagggctgcc agatgaggca
     2761 gccctgagcc tgccagtagt ccagcaggcg gaggatcatt tgttggaagt tgaggggggt
     2821 tgtcatagtt gtcagtgggg gattatagca cggtgtagaa gagctcacgc aaaggcgcgg
     2881 aggagctaag gcgcaaagga aaacaaactg cttcgcttgg cgttcctggc gttctttggc
     2941 tgaggttctt tcttagcgcc tttgcccctt cgcgcctttg cgtgagcctc ttactcttcc
     3001 gccaacaact ccgcttcgcg gcgcaggcgg tggaggaagt ccacggactt cagttcgcgc
     3061 tccaggatgt gggtcaggta gtcgtagagg acgcgctcga cctcgtgcag cacggccggc
     3121 tggaggcgca actggctgac agtggcccac ggccgcgtct gcaagtagcg catcacctta
     3181 acggccacgg ccgagacggg catggcgcgc gggtcggtcg ggccgcaggc ggggcagagc
     3241 aggccgccca gctcggggct gaagaactgg tcttgctcgg cgatggcctc gccgcaggcg
     3301 acgcagttga acagccgcgg ctggaagccg gtcagcgaca gcagccgcag ttcgtagaag
     3361 cgggccagca gcagcggctg gtcggccgcg gccagcgttt ccagagccag cgtcagcagc
     3421 tcgtagatgt tgcggtgggg gtcttcgtcg acggtgaagc gatcgagcag ttccacggcg
     3481 tagttggcat aggttgcccg caccaggctc tcgcgcagcg gcgcgtaggt gtccaccgtc
     3541 tccgcctggg taacgatgtc gaggtcgcgc ccctcggcca ggaggaactt gctgcgcatg
     3601 aatagctcta cgtggccggt cttgcggctc tggggcttgc gcgcgccctt ggcaatggcg
     3661 cgcatcttgc cacgttcagg gctgaaaaga gtcaacagcc ggtcagcttc gccgaagtcg
     3721 ctgcggcgga cgacgatggc ctcggtacgg taggtgcgtt cgcgtggcat gagaggattt
     3781 tacctcaaaa tgggcagaag ggcgcggttg tcgggaaggc cataggccca cgcgcccggc
     3841 gggcatcagc gagcggccgc tctgcgccct gtcgccggcg gcgatcggct ccctcgactg
     3901 caccccggac ggtacggagt tgttcctcga cagcgccctg gcccccacgc cgacgcccgc
     3961 gccggccgtc tggcgagccg tggccattcc cctgccgccg ctgcccgatg tgggcgacgc
     4021 ggcgctcgtg acgccggagg aggccgaaga ccagccaccg gcgcaactct tcggccactt
     4081 cgccgagggt acaccggtgg agttgctgcc gccacaggcc gcgccccagg tcttcctgac
     4141 gccgccgcgc aacgcggact acctgcgcct catcgtcgtc aaggacagca actacgtggg
     4201 cgagccgcac cagatacaga ttgtagtaga atagcctctg gacatgcgcg aggaacgaga
     4261 ggggttgaat gaatcagaaa atgatcggcc gttttatcgt aaccgacccc gagatatgcc
     4321 atggcacgac acaaaagtga cccatccttg gccatagagg gctagtgcct actccacaac
     4381 agtctctgct tgaccacgcc acagacagcc tatcttgccg ggccaacctt cgttggctca
     4441 tcggcggctc cgcctccggc aagtcaacca tcgcccgcac cctcggcgcg cggacgggca
     4501 tcgccgtcta cgacatggat gaggccgtct tcggccgctt ccactttgac cccatccgcc
     4561 acccggcgac gacggcctgg ttcaccgccg ccaacccgtt agcgtggatg ctctcccggc
     4621 cgtgggcgga gttcgaggct ctctaccggg ctaacaacgc cgagatgctc gacctgctgg
     4681 ccggcgacct ggccggccgg cccgacgcgc cgctgctgat cgacggcggc atcacccacc
     4741 cgtcggtgct gacgcaagtc atccccgccg ggcgcatcgt ctgcctggag acggacgacg
     4801 cgcgccgccg ccatgagtgg gcgacggccc ccggccgggc cggcatgagg gccgaaatcc
     4861 tggccctgcc cgacggcgca gcgctatggc ggcggttcct tgactacgac caacggctga
     4921 cggacacgat tgggcgcgag agtcgggacg ccggcattcg cgttctggcc tggggcgagg
     4981 gcatcggcgt cgaggatgtg
//
LOCUS       DAOSMQ010000223         4977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17032_length_4977_cov_5.509752, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000223 DAOSMQ010000000
VERSION     DAOSMQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17032_length_4977_cov_5.509752"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PK829_08505"
     CDS             <1..1333
                     /locus_tag="PK829_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41294.1"
                     /translation="VLLTAAAALLWRNDPRGLPAGDLTWDFAQMAYLHHDRGGVAFEN
                     GIRLYEYGYSAEEIAAGETLVVTLSVTPGDGRAATLALVTPASVRPPAEGAPAPPVVA
                     AATRPLDTAAPVFRLSIPADAPPGLYVPRLTLDGARPLLPSGGTRGDLFLRPVRVKAG
                     IRNGEGDGRPATGNLDVRAMSLTMRDATTLDGHFAWYTDRPLGARYQFSLRLQDATGA
                     TLAQLDAQPGYGFQPSTLWAAGQWTADWLALRLPDAASAGAYPLVMRLYDAGSGQTVL
                     TRRVGMAEWAGGAWIARLHEPNFVLPGGLRPAAATFSGDGRALIDLRGYQLAQDGGAL
                     RLTLIWQAATGAPGDFTRFVHLLNSAGAIVAQSDGAPAGDSYPTGQWVAGEVVSDTVT
                     FDLSVLPPGEYRLATGFYAPSEGLPRLDATTPAGPLPDGRVLLPEAISIAK"
     gene            complement(1352..2608)
                     /locus_tag="PK829_08510"
     CDS             complement(1352..2608)
                     /locus_tag="PK829_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU41295.1"
                     /translation="MPATSDAIERALREDTGRVLAILIAAFGDIDLAEDALQEALLVA
                     VERWPAGGLPDNPAAWLVTVARRKAFDRLRRDRTLAEKMPLLADLATDPFDDPAAMDD
                     TPIPDERLKLVFTCCHPALALEARVALTLRLLGGLDTAEIARAFLVATPAMNQRLTRA
                     KAKIRAAGIPYEVPPLTAVPERLDGVLHVLYLIFNEGYAATSGDAHIRADLCAEALRL
                     AATLVDLLDARPELGPQPEAMGLLALLRLHDSRRAARLDAAGDIILLEDQDRRLWNRA
                     AIDAGLSLLDRALALRRPGPYQIQAAIAALHARAATAADTDWQQIAALYDALRRVAPT
                     PVVELNRAVAVGMARGPLAGLAQLQRPELAAALDGYHWYHAAVADFLRRAGYRPEARA
                     AYVRALSLCDNRAEQIFLARRIAETS"
     gene            complement(2681..3031)
                     /locus_tag="PK829_08515"
     CDS             complement(2681..3031)
                     /locus_tag="PK829_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOU41296.1"
                     /translation="MQYMLLIYVDPQTYAGVTEEQLQTEMAEYYAFGPSLRERGIEVS
                     GEGLQGIETATTLRVRDGQALITDGPFAETKEHLGGFYLIDVANLDEAIEVARRIPDV
                     RRGSVEIRPVMVYN"
     gene            complement(3125..4120)
                     /locus_tag="PK829_08520"
     CDS             complement(3125..4120)
                     /locus_tag="PK829_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOU41297.1"
                     /translation="MTAPLARALRFHRFGPPAEVLCLEELPVEEPGPGQVRLRLSHRP
                     INPSDLLTIAGQYGRLPRLPATPGLEGVGRVEAVGEGVRGWRVGDRAIPLGAGGTWRE
                     SQLVAAAGLLPVPDAVDDATAAQFVVNPVTAWVMLEQELALQPGDWVVQTAAGSALGR
                     MVIQLAQLRGYRTINFVRRPEQVAELRDLGGDAVFATGEPDIVARVRELTGGQGARGA
                     LEAVGGETGALALRCLQPGGVMLVYGLLGGEPLPLHNGEMLFRGLTIRGYWLSYWFQQ
                     TPPERVATTLQGLMALLAEGRLELAVAASYDLADFRAAVAHAERPGRQGKILLTG"
     gene            complement(4117..4596)
                     /gene="bcp"
                     /locus_tag="PK829_08525"
     CDS             complement(4117..4596)
                     /gene="bcp"
                     /locus_tag="PK829_08525"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOU41298.1"
                     /translation="MLQIGDPAPDFELLSDENQPVTLGDLRGQRVVLFFYPKADTPGC
                     TVQACGFRDRFPIIEAGNATVIGVSPDMPAALARWRAKEQLPYTLLSDPDHVLADAYG
                     VWGEKTAYGRSYMGIIRSHAIIGPDGRIEDLRIRVSPEESVQAAVDFLAAHGENGPA"
     gene            complement(4611..>4977)
                     /locus_tag="PK829_08530"
     CDS             complement(4611..>4977)
                     /locus_tag="PK829_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41299.1"
                     /translation="ADVIDVDELNSGERREGLYTGYLVFLRKLGTGVMVFAVGQLLSA
                     TGYASSTTGSAFMEQPAAALVVMRALVTALPAIALGLSLLLAWRFPIDRTSHEAVRRA
                     LDERRAAREAGATLPSPNP"
BASE COUNT          746 a         1741 c         1771 g          719 t
ORIGIN      
        1 cgtcctgctg acggccgcgg ccgccctcct ctggcgcaac gacccgcgcg gcctgcccgc
       61 cggcgatctg acctgggact tcgcccagat ggcctatctg caccacgacc gcggcggcgt
      121 ggccttcgag aacgggatac ggctatatga gtacggctat agcgccgagg aaatcgccgc
      181 cggggaaacg ctggtcgtga cgctcagcgt gacccccggc gacggccgcg cggccacgct
      241 ggcgctggtg acgccggcca gcgttcgccc gccggccgaa ggcgcgcccg cgccgcccgt
      301 tgtcgccgcg gcgacacggc cgctggacac ggccgcgccg gtcttccgcc tgagcattcc
      361 cgccgacgcg ccacccggcc tctacgtgcc tcgtctgacg ctcgacggag cgcgcccgct
      421 gctgccctct ggtggcacgc gcggcgatct gttcctgcgg ccagtgcgcg tcaaagcggg
      481 aattaggaat ggggagggcg acgggcggcc agcgaccggc aacctggacg tgcgggccat
      541 gagcctgacc atgcgcgacg cgacaacgct cgacggccat ttcgcctggt acaccgaccg
      601 gccgctgggg gcgcgctacc agttctcgct gcggctgcaa gacgccaccg gcgcgacgct
      661 ggcccaactt gacgcccagc ccggctatgg cttccaaccg agcacgctct gggcggcagg
      721 gcagtggacg gccgactggc tcgccttgcg cctgccagac gcggcttcgg cgggcgcgta
      781 cccgctggtt atgcgcctct acgacgccgg cagcgggcaa acggtgctga cgcggcgggt
      841 ggggatggct gagtgggccg gcggcgcgtg gatagcgcga ctacacgaac cgaactttgt
      901 cctgccgggc ggcctgcgcc ccgcggcggc gacctttagc ggcgacggcc gggcgctcat
      961 cgacctgcgc ggctatcaac tggcgcaaga cggcggcgcg ctgcggctga cgcttatctg
     1021 gcaggccgcg actggcgcgc cgggcgactt cacccgcttc gtccacctgc tcaacagcgc
     1081 cggggccatc gtcgcccaga gcgacggcgc gccggccggc gatagctacc ccaccggcca
     1141 gtgggttgcc ggcgaggtcg tcagcgacac ggtgacgttc gacctgagcg tcctgccacc
     1201 gggcgagtac cgcctggcca ccggcttcta cgcgcccagc gagggcttgc cgcgcctgga
     1261 cgcgaccacg ccggccggcc ccctgcccga cggccgcgtt cttctgccag aagccatctc
     1321 catcgccaag tagactcgcc gcgccctcgt cctaactcgt ctcggcgatg cggcgggcga
     1381 ggaaaatctg ctcggcgcgg ttgtcgcaca gtgacagggc gcgaacgtag gcggcgcggg
     1441 cctctggccg gtagccagcg cggcgcagga agtcggccac agcggcatgg taccagtggt
     1501 agccgtcgag cgccgcggcc agttccggcc gttgcagctg cgccagcccc gccagcggcc
     1561 cgcgagccat gcccacggcc accgcccggt tcaattccac caccggcgtc ggcgcgacgc
     1621 ggcgcaaggc gtcgtagagg gcggcgatct gctgccaatc ggtgtcggcc gcggtggcgg
     1681 cgcgggcgtg cagcgcggcg atggccgcct gaatctggta ggggccgggc cgccgcagcg
     1741 ccagcgcccg gtcgagaagg gatagcccgg cgtcgatggc cgcgcggttc cacagccgcc
     1801 gatcctggtc ttccagcagg atgatgtcgc cggcggcgtc cagccgggcg gcgcgacgcg
     1861 agtcgtgcag ccgcagcagc gccagcagcc ccatcgcctc cggctgcggc cccagttccg
     1921 gccgggcatc gagtaagtcc accagcgttg ccgccaggcg caaggcttcg gcgcagagat
     1981 cggcgcggat gtgggcgtcg ccggaggtgg cggcgtagcc ctcgttgaag ataaggtaga
     2041 ggacgtggag aacgccgtcg agccgttcgg gcacggcggt gagcggcggc acctcgtagg
     2101 ggatgcccgc ggcgcgaatc ttggccttgg cccgcgtcag gcgctggttc atcgccggcg
     2161 tcgccaccag gaaagcgcgg gcgatctcgg ctgtatccag cccacccaac agccgcagcg
     2221 tcagggccac gcgcgcctct aacgccagcg ccggatggca acaggtgaag acgagcttca
     2281 gccgttcgtc ggggatgggc gtgtcgtcca tggccgcggg gtcgtcaaat gggtcagtgg
     2341 ctagatcggc cagcagcggc atcttctcgg ccagcgtccg gtcgcggcgc aggcggtcga
     2401 acgccttgcg ccgggcgacg gtcaccagcc aggcggcggg gttgtcgggc aggccgccgg
     2461 ccggccaacg ctccacggcg acgagcagcg cctcctgcaa cgcatcctca gccaggtcga
     2521 tgtcgccgaa tgcggcgatg aggatggcca ggacgcggcc ggtgtcttcg cgcagggcgc
     2581 gctcgatggc gtcgctggtg gcgggcatcg gttggccggc cccgcgcggc ccgcgccggc
     2641 caccggccag cgtgggcagc gcgagagttt tgccggacgc ctagttgtag accatcaccg
     2701 ggcgaatctc gaccgagccg cgccggacgt cggggatacg cctggcgacc tcgatggcct
     2761 cgtccagatt ggccacgtcg atcaggtaga agccgcccag gtgttccttc gtctcggcaa
     2821 aggggccgtc agtgatgagc gcctggccgt cgcggacgcg cagtgtcgtc gccgtctcaa
     2881 tgccctgcaa gccctcgccg ctcacctcga tgccgcgctc gcgcagcgac gggccaaagg
     2941 cgtagtactc ggccatctcg gtctgaagct gctcttcggt cacgccggcg taggtctgcg
     3001 ggtccacgta gatcaacagc atgtattgca tcgttcgtct cctgaacatg atatggcgct
     3061 tgtgcgcgct ttctatggta atgacgaatg accaacagcg atttcgacaa cggccgcgaa
     3121 ccaattaacc ggtcagcaga attttgccct gccggcctgg ccgctcggcg tgggccacgg
     3181 cggcgcggaa gtcggccaga tcgtagctcg ccgcgacagc caattccagc cgtccctcgg
     3241 ccagtagcgc catcagccct tgcagcgtcg tcgccacgcg ctccggcggc gtctgctgga
     3301 accagtagct cagccaatag ccgcgtatgg tcagaccgcg gaagagcatc tcgccgttgt
     3361 ggagcggcag cggctcgccg cccagcaagc cgtagacgag catgaccccg cccggctgca
     3421 agcagcgcag cgccagcgcg cccgtctcgc cgccgacggc ctccaacgcc cctctggctc
     3481 cctggccgcc ggtcaactcg cggacgcgcg cgacgatatc cggctcgccg gtggcgaaaa
     3541 cagcgtcgcc gcccaggtcg cgcagttcag ctacctgctc tggccggcgg acgaagttga
     3601 tcgtccggta gccacgcaat tgggccagct ggatgaccat ccggccgagg gccgagccgg
     3661 cggccgtctg cacgacccaa tcgccgggct ggagggccaa ttcctgctcc agcatcaccc
     3721 aggcggtcac cggattgacg acgaactggg ccgccgtggc gtcgtctacg gcgtcgggca
     3781 cgggcagtag gccggcggcg gcgacaagct ggctttcgcg ccacgtgccg cccgcgccca
     3841 gcgggatggc ccggtcgcca acgcgccagc cgcgcacgcc ctcgcccacg gcctcgaccc
     3901 ggccgacgcc ctccaacccc ggcgtggccg gcagccgcgg caggcggccg tactggccgg
     3961 cgatggtcag cagatcagac gggttgatgg gccggtggct caggcgcagg cgcacctggc
     4021 cggggccggg ttcctccacc ggcagttctt ccaggcacag cacctcggcc ggcgggccga
     4081 agcgatggaa acgcagggcg cgggccagcg gcgcggtcat gccgggccgt tctcgccgtg
     4141 ggcggccagg aaatcgaccg ccgcctgtac gctttcctcc ggactgacac ggatgcgcag
     4201 gtcttcgatg cgcccgtcgg ggccgatgat ggcgtggctg cggatgatgc ccatgtagct
     4261 gcggccgtag gcggttttct cgccccagac gccataggca tcggccagaa cgtggtcggg
     4321 gtcagacagc agcgtgtagg gcagttgttc cttggcgcgc cacctggcca gcgcggcggg
     4381 catgtcgggg ctgacgccga tgacggtcgc gttgccggcc tcgatgatcg ggaagcgatc
     4441 gcggaagcca caggcctgga cggtgcagcc gggcgtgtcg gccttgggat agaagaagag
     4501 cacgacacgc tgcccgcgca ggtcgcccag cgtcaccggc tggttttcgt cggacagcag
     4561 ttcaaagtcg ggggcgggat cgccgatctg taacatgtga gtgactcctt ttatgggttg
     4621 ggtgaaggca aggtcgcgcc ggcttccctg gcggcgcggc gttcgtccag cgcgcggcgg
     4681 acggcttcgt ggctggtccg gtcgatggga aagcgccagg ccaggaggag cgacagcccc
     4741 agagcgatgg caggcagcgc ggttaccaga gcgcgcatga ccaccagcgc ggcggctggt
     4801 tgttccataa aggcgctacc ggtagtggaa ctggcataac ccgtggccga cagcagttgg
     4861 ccgacagcaa agaccatgac gcccgtgccc agcttgcgca ggaaaaccag gtagccggtg
     4921 tatagccctt cgcgccgctc accgctattc aactcatcca catcgatgac gtcggcg
//
LOCUS       DAOSMQ010000224         4950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17153_length_4950_cov_4.880695, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000224 DAOSMQ010000000
VERSION     DAOSMQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17153_length_4950_cov_4.880695"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="dapB"
                     /locus_tag="PK829_08535"
     CDS             complement(<1..743)
                     /gene="dapB"
                     /locus_tag="PK829_08535"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOU41300.1"
                     /translation="MTIKVCLAGATGWAGSALARHIAHTEDIELVAAVSRTHAGRILG
                     NVLGEPRLIGPILATAEEALAYPCDVFFEYTRPDVAKANVLAALNRGAHVVIGTSGLT
                     DDDYAMLASVAEARGRGVLAAGNFALTAVLLQKFAEMAARLIPQWEIIDYAADGKRDA
                     PSGTVRELAARLSRIRPAELTVPLEETQGVVETRGARLGGAQVHSVRLPGYTISAEIL
                     FGMTDQRLSIRHDSGNSAEPYVDGALLAIR"
     gene            complement(768..2153)
                     /locus_tag="PK829_08540"
     CDS             complement(768..2153)
                     /locus_tag="PK829_08540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU41301.1"
                     /translation="MIPTTADIVICGAGIAGISGAYHLAARHGIGDILLVEEGSPLML
                     TSDKSTEAYRNWWPGPGDAMVRFMNRSIDLLEAFAAASDNLFHLNRRGYVFLTADPAR
                     AEVYRRSAEESSALGAGELRIHRGRPDDPPFPPPVAEGYAPELGGADLVLDPALIRQR
                     LPFVTPDAVAMLHPRRCGWLSAQQLGMWLLDEARARGVRLVSGRVTAIDMAGGRVRGV
                     TVRAAGGEQRIETGVFVNAAGPLVGAVSRLLGVELPIFNELHGKVAINDPLGIVPRDA
                     PLLIWSDPVLLPWADEEREELAAAEETRWLTEPFPAGVHFRPEGGPGAQTLLLLWTYD
                     VKPAEAVWPTRFAPEYAEVVVRGLARMVPELAVYAGNFGRPIIDGGYYCKTQENRPLI
                     GPLPVEGAYVFGALSGYGIMASQAGADLLAAHIAGSDLPAYAPAFALSRYDDPAYRAL
                     LNSWDPTTGQL"
     gene            complement(2343..4199)
                     /locus_tag="PK829_08545"
     CDS             complement(2343..4199)
                     /locus_tag="PK829_08545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOU41302.1"
                     /translation="MPLLPGERLHNRYRVVSLLASGPYGAVYRAYDAADRRSVAIKEY
                     LDPSVDIQRRFRAEARRLAALSHPQLPDTLDHFALDEGQYLVSAYIDGVSLAELLAQY
                     GPLPSDLIIPWLQSASHPLAYLHAQKQLHLNVKPANIRLTPAGEVFLVDSGLPGLGVR
                     PHTAGFGAPEHQAQGAVGPEADIYSLGATLYALLTGRTPPNALSRETGLSDLVPAREV
                     NPDVEPYLSIVAGRAMSLRADARYESVEAFARALERPAGRPAPEVSPLRRTEPVETSA
                     RPAAAPRRPTRTRRRLERRTALALAVGGLFLLAFVAFLLVTNLDREQPVAGPQATQTL
                     QSAVAAALTEFAPTPTPSPEPTIAPTPTPRPFITGTGSRMIYVPGGEFSLGNDDSEEN
                     DEKPAVRVQIDSFFIDETEVTNAAYALCVDAGVCPRPDRAGATYHPSYFGDPAFDDYP
                     VINVSWFDAEAFCEWRDARLPTEAEWEYAASFDPMEGIKFLHPWGDSFDGERLNFCDV
                     NCRRDGPDAVWDDGFNDTAPVGSYPDGRSPLGVYDMLGNVMEWVGDWYDFNAYDAIAD
                     TNPRGPTEGDFKSLRGGSWLSPSAELNVSARGNFEPTVSQANLGFRCAMPPP"
     gene            complement(4233..4862)
                     /locus_tag="PK829_08550"
     CDS             complement(4233..4862)
                     /locus_tag="PK829_08550"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU41303.1"
                     /translation="MLRADHWSLPMDDNDPFSTLTTLPPEQRWRGQAAPVPVVVALIR
                     RAHDDGEQLLLIRRAAGPYTGHWALVGGKWDFGETLAEAIVREVREETGLATALVAVR
                     GLISERVTPWSPAELGAHFLLLVCDLVVRGGEAAEQSEGLVAWFDHAAIDALHAAGTI
                     IPSDYAMIREFAAATGCAPYVEVEMRATVGGAAGSLSELLRFRRHEHDD"
BASE COUNT          757 a         1661 c         1809 g          723 t
ORIGIN      
        1 cgaatggcca gcagcgcccc gtccacatac ggctcggcgc tgttgccgga gtcgtggcgg
       61 atggatagcc gctgatccgt catgccgaag aggatctcgg ccgagatggt atagccgggc
      121 agacggacgg agtggacttg cgcgccgccc aggcgcgcgc cgcgcgtctc gacgacgccc
      181 tgtgtttcct ccagcggaac ggtgagttcc gcggggcgaa tgcgcgacag gcgcgcggcc
      241 agttcgcgca ccgttccgct gggcgcgtcc cgcttgccgt cggcggcgta gtcgatgatt
      301 tcccattgtg gaatcagccg cgccgccatc tcggcgaatt tctgcaggag gacggccgtc
      361 agggcgaagt tgccggcggc tagcacgccg cgcccgcggg cctcggcgac gctcgccagc
      421 atggcatagt catcatccgt caggcccgac gtgccgatca cgacgtgcgc gccacgattc
      481 agagccgcca ggacgttggc tttggccacg tccggccggg tgtactcgaa gaaaacgtcg
      541 cagggatagg ccagcgcttc ttcggccgtg gccaatatcg ggccgatcag gcgcggttcg
      601 cccaacacgt tgcccagaat gcggcccgcg tgcgtgcgcg agaccgcggc cacgagttcg
      661 atgtcctcgg tgtgggcgat gtggcgggcc agcgccgacc cggcccaccc ggtcgcgccc
      721 gccagacaaa ctttgatggt catagtgcct cgcgagtcat cccaccctta caactgcccc
      781 gtcgtcgggt cccaactatt caacagcgcc cgataggccg ggtcgtcgta gcggctgagg
      841 gcgaaggcgg gcgcgtaggc tggcaagtcg ctgccggcaa tgtgggcggc cagcaggtcc
      901 gcgcccgcct gggaggccat aatgccatag cccgacagcg ccccgaagac gtaggccccc
      961 tctactggca gcgggccaat gagcggccga ttctcctgcg tcttgcagta gtagccgccg
     1021 tcgatgatcg gccggccgaa gttgccggcg tagacggcca gctccggcac cattcgcgcc
     1081 agcccgcgca ccactacctc ggcgtactcc ggcgcgaagc gcgtcggcca gacggcctcg
     1141 gccggcttga cgtcgtaagt ccacagcagc agcaacgtct gcgcgccggg gccgccctcc
     1201 ggccggaaat ggacgccagc ggggaacggc tcggtcagcc agcgcgtttc ctcggccgcg
     1261 gccagttcct cgcgctcttc atctgcccag ggcagcagca ccgggtcgct ccagatgagc
     1321 agcggcgcgt cgcgggggac gatgcccaat gggtcattga tggccacctt gccgtgcagc
     1381 tcgttgaaga tgggcaactc gacgcccagc agccggctga ccgcgcccac cagcggcccg
     1441 gcggcattga cgaagacgcc cgtctcaatg cgctgctcgc cgccggccgc gcggacggtg
     1501 acgccgcgca cccgaccgcc ggccatgtcg atggccgtca cacggccact gaccagccgc
     1561 acgccgcgcg cccgcgcctc gtccagcagc cacatgccca gctgttgggc gctcagccag
     1621 ccacagcggc gcggatggag catggccacc gcgtcggggg tgacgaatgg caagcgttgc
     1681 cggatgagcg ccgggtcgag taccaggtcg gccccgccca attccggcgc gtagccctcg
     1741 gccacggggg gcgggaaggg cgggtcatcg ggccggccgc ggtggatgcg tagctcgccc
     1801 gcgcccagtg ccgatgactc ctcggctgag cgccggtaga cctcggcccg cgcggggtcg
     1861 gcggtcagga agacgtagcc gcggcggttg aggtggaaga ggttgtcgct ggcggcggcg
     1921 aacgcctcca acaagtcgat gctgcgattc atgaagcgca ccatggcgtc gccggggccg
     1981 ggccaccagt tgcggtaggc ctcggtcgat ttgtcgctgg tgagcatgag cggcgacccc
     2041 tcttcgacga gaaggatgtc gccgatgccg tgacgcgcgg ccaggtgata ggcgcccgag
     2101 atgccggcga tgcccgcgcc acagatgacg atgtcggctg ttgtggggat catgggcttg
     2161 ttttaacgca aggacgcaaa gacgcaagag gatcagggtt tccccaaagt cacccaggcg
     2221 cgccgacctg acaggtgggc gcgccacgaa tggcactgcc gccagagaac gactggccgc
     2281 ccgcctgtca ggtcaaattg gggcgcgggc cgtcgcccac agcctgtcgt ctgtggcccg
     2341 cctcatgggg gcggcatggc gcagcggaag ccgaggttgg cctgcgacac ggtcggctcg
     2401 aagttgccgc gggcgctgac gtttagctcc gccgacggcg acagccacga gccgccgcgc
     2461 aacgacttga agtcgccctc cgtcgggccg cgcgggttgg tgtcggcgat ggcgtcgtag
     2521 gcgttgaagt cgtaccagtc gccgacccac tccatgacgt tgccgagcat gtcgtagacg
     2581 cccaggggcg agcgaccgtc ggggtagctg ccgacgggcg cggtgtcgtt gaagccgtcg
     2641 tcccagacgg catcggggcc gtcgcggcga cagttgacat cgcagaagtt gagccgctcg
     2701 ccgtcgaacg aatcgcccca ggggtggagg aacttgatgc cctccatggg gtcgaaggag
     2761 gcggcgtatt cccactcggc ctcggtcggc agtcgcgcgt cgcgccactc gcagaaggct
     2821 tcggcgtcga accaactgac gttgatgacc ggatagtcgt cgaaggccgg atcgccgaag
     2881 tagctggggt gataggtcgc cccggcgcgg tcgggccgcg ggcagacgcc ggcatcgacg
     2941 cacagcgcgt aggcggcgtt ggtgacctcg gtctcgtcga tgaagaaact gtcgatctgc
     3001 acgcgcacgg ctggcttctc gtcgttctcc tcgctgtcgt cattgcccag gctgaactcg
     3061 ccgccgggga cgtagatcat ccgcgagccg gtgccggtga tgaacggccg tggcgtcggc
     3121 gtgggcgcga tggtcggctc cggcgagggc gtcggcgtgg gcgcgaactc ggtcagggcg
     3181 gcggcaacgg ccgattgcag cgtctgcgtc gcctgcggcc cggcgacggg ttgctcccgg
     3241 tccaggttgg tgacgagaag gaaggcgaca aaggccaaca ggaagaggcc gcccacggcc
     3301 agggccaatg ccgtgcgccg ttccaggcgg cggcgcgtgc gtgtgggccg gcgcggcgcg
     3361 gcggcgggcc gggccgaagt ctccaccggc tccgtgcgcc gcagcgggct gacctccggc
     3421 gcggggcggc ccgccggccg ctccagcgcg cgggcaaagg cctcgacgga ctcgtagcgc
     3481 gcgtccgccc gcagcgacat ggcccgccca gcgacgatgg acaggtatgg ctcgacgtcg
     3541 gggttgacct cgcgggcggg caccaggtcg ctcaggcccg tctcgcggct gagggcgttg
     3601 ggcggcgtcc gcccggtcag cagggcgtag agcgtcgccc ccaggctgta gatgtccgcc
     3661 tctgggccaa ccgcgccctg ggcctggtgc tccggcgcgc cgaagccggc cgtgtgtggc
     3721 cgcacaccca gccccggcag gcccgaatcg accaggaaga cctcgcccgc gggcgtcagg
     3781 cggatattgg ccggcttgac gttgaggtgc agttgcttct gcgcgtgcag gtaagccagc
     3841 gggtggctgg cggactggag ccaggggatg atcaggtctg acggcagcgg gccgtactgc
     3901 gccagcaact cggccaggct gacgccgtcg atgtaggcgc tgacgagata ctggccctcg
     3961 tcgagggcga aatgatcgag cgtgtccggc aactgcgggt ggctcagcgc cgccagccgc
     4021 cgcgcctcgg cccgaaagcg ccgttggatg tcaacggacg ggtcgaggta ctccttgatg
     4081 gccacgctgc gccggtcggc ggcgtcgtag gcgcggtaga cggccccata tggcccggag
     4141 gccagcaggc tgacgacgcg gtagcggttg tggagacgtt cgccggggag aaggggcata
     4201 ggcgttagag aagccggccg cgcgggcgag gcctagtcgt catgctcgtg gcggcggaag
     4261 cgcagcagct cgctcagcga cccggccgcc ccgccgaccg tggcgcgcat ttcgacttcg
     4321 acgtaggggg cgcagccggt ggccgcggcg aactcgcgga tcatggcgta gtcgctgggg
     4381 atgatcgtcc cggcggcgtg gagggcgtcg atggcggcgt ggtcgaacca ggctacgagg
     4441 ccctcgcttt gctcggccgc ctcgccgccg cgcacaacca ggtcgcagac cagcagcagg
     4501 aagtgggcgc ccaactcggc cggcgaccac ggcgtgacgc gctcgctgat cagcccgcgc
     4561 acggccacca gcgctgtcgc caggcccgtc tcctcgcgca cctcgcggac gatggcctcg
     4621 gccagcgtct caccgaagtc ccacttgccg ccgacgagcg cccagtgacc ggtgtatggc
     4681 ccggcggcgc ggcgaatgag cagcagctgc tcgccgtcgt cgtgcgcccg ccggatcagg
     4741 gcgacgacca cgggcacagg ggcggcctgc ccgcgccagc gctgttcggg gggcagcgtc
     4801 gtcagggtgc taaagggatc gttatcgtcc atcggtagtg accagtggtc agcacggagc
     4861 atgaaccact ggcctcatca ctgaccactt acttgcgttc gccaaagtat tgtagcagca
     4921 acgcgtggac gtaggtatag ccgccgccga
//
LOCUS       DAOSMQ010000225         4949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17158_length_4949_cov_3.956273, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000225 DAOSMQ010000000
VERSION     DAOSMQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17158_length_4949_cov_3.956273"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="PK829_08555"
     CDS             <1..1692
                     /locus_tag="PK829_08555"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU41304.1"
                     /translation="PFYIEELVKMLIEQGVIERVGVDPVESPAGAPALKVAPERWHIH
                     ADRLPTIQVPSTLTGLLQARLDGLPREERAVLQRAAIVGRVFWDDAVRTLSDTAAQLT
                     SAAESPDSPTNGATGGSNGHIDPRALNALLDSLTRRELVVRRERSVFAGTHEYTFKHN
                     LLREVTYQTVLLRARRQHHARVARWLELNAGARLGEYLGLIAEHYALAGANARAAAYL
                     ERTGDSAQRSGAYHSARAAFERALSLLQAEDTPAETAISGLRLKLGAVAWNLGDFPAA
                     ERALFYARDTARRAGDGHAQALALYWLSRVAISRGDYAQARMVLAYALPLARAADAET
                     LAQVLYGVADVAWKTGDMDTLANYIRESLNLARALDNTALELMALNRLGTMAYLQGDL
                     ETAHTYYRTCLELAQTTGNLEREATALMNLGALAHRGGDPDAGLAHFRDALARYRELG
                     RTEQVVMALGNAAEADIDRGDLGQARSDLGEGMALAWRLGLLPRVTVLLFVYGRLLIA
                     EGQHERAAAVLRLVLDHPATESQTRPQALALLNLLGQPGDGPAADLETIVAEVLA"
     gene            1854..4295
                     /locus_tag="PK829_08560"
     CDS             1854..4295
                     /locus_tag="PK829_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU41305.1"
                     /translation="MISKRAPLSRFVPARRIHVAAVALFAALVLLLGHPPAQAAPTSP
                     AVAPMVSPPTNQIIVQFAPAGEAALLSANTGQLLDRLSTAAGLTVTLERPMSGNAYVV
                     GLPARIDEAAVAAMAARMAALPEVVYAEPDAIMQIIASPPLAEAPAANLTPDDTRFAD
                     QWHYRYVPGVEEGLNLLPAWGITTGSAATVVAVIDTGIRGHADLAGRTVPGYDFIADA
                     PTANDGNGRDNDPTDPGDWSTAGQCFPGSTARDSSWHGTHVAGTIGAASNNGSDVAGV
                     NWKAKILPLRVLGRCGGFLSDIVDATRWAAGLAVPGAPANANPADVINLSLGGSGACG
                     ASYQNAFNDLAAAGVVSVVAAGNSAINAANARPANCNNVITVAATSKTGDQTYYTNFG
                     ALVEIAAPGGEQFAPNDAGGVLSTLNAGLTGPGADDLRYYQGTSMASPHVAGLVSLLL
                     GEDPSLTPAEVLDILQTTARPFPAGSSCDTSTCGAGIADAFAALSALNPGLSAPLLIT
                     PTNGQTLATAEVAFEWGGVENATGYRLQVATDSDFNDLVVNDASLLNTSATKTLPGEG
                     TYYWRVRALGDEDGPWSEVWSFTIAFPACTVPDAPMLSDPADGSSVGDLTPNFQWGAV
                     MDATTYDIRVSKQPDVSNPIISDSSATAGYTVTTSLTDNETYYWRVRAHNNGGACNET
                     GPWSEVWQFSIAVSTCPTPAIPELLFPEDASTVNAAPTLTWADATDATEYEVRIGIEP
                     DVADTLKQATVTDTEYAIDLTLTDGTTYYWMVRAGNSDGSCNTLGDWSAVWSFTYEKP
                     QPVTYLAYLPAVFGQ"
     gene            complement(4365..>4949)
                     /locus_tag="PK829_08565"
     CDS             complement(4365..>4949)
                     /locus_tag="PK829_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOU41306.1"
                     /translation="TEDFQRFGGTQPLDRPYLDSSVLTVARKRVGWLLLLFVTETLTG
                     SVLRHFEAEMAAVVSLAFFIPLLIGTGGNAGSQTTSTIIRALAVEDLDKRGLMRPLMH
                     ELSVGLLLGLVMGIVAYGRALLWGTGQPVALTVSVSIFAIVVWANVLGSLLPIIANRL
                     RIDPTVVSGPAMSTLVDATGLFIYFTVAGLILAL"
BASE COUNT          842 a         1849 c         1558 g          700 t
ORIGIN      
        1 cccttctaca tcgaggaact ggtcaagatg ctcattgagc aaggcgtcat cgagcgggtg
       61 ggggttgatc ctgtcgaaag cccggccggc gcgccggcgc tcaaggtcgc gccggagcgc
      121 tggcacatcc acgccgaccg cctgcccacc attcaggtgc cctccacgct gaccggcctg
      181 ctgcaagcgc gcctcgacgg cttgccgcgc gaggagcggg cggtcttgca gcgcgccgcc
      241 attgtcggcc gcgtcttctg ggacgacgcg gtgcgcaccc tgagcgatac cgccgctcag
      301 ctgaccagcg ccgccgaatc gcccgatagc ccgaccaacg gcgcgaccgg agggagcaat
      361 ggccacatcg atccgcgcgc cctcaatgcc ctgctcgact cgctcacccg gcgcgaactt
      421 gtcgtccgcc gcgaacgttc tgtttttgcc ggcacccacg aatacacctt caagcacaac
      481 ctgttgcgcg aagtgaccta ccagaccgtc ttgctgcgcg cccggcgaca gcaccacgct
      541 cgcgtcgccc gctggctgga gctaaacgcc ggcgcgcgtc tgggcgaata cctcggcctc
      601 atcgccgagc actacgccct ggccggggcc aacgcccgcg ccgccgccta tctggagcgt
      661 accggcgaca gcgcccagcg cagcggcgcg taccactccg cccgcgccgc cttcgagcgt
      721 gccctgtcgc tcctgcaagc tgaggacacg cccgccgaga cggccatcag cggcctgcgc
      781 ctcaagctgg gagccgtggc ctggaacctg ggcgactttc cggccgccga acgggcgctc
      841 ttctacgccc gcgacaccgc ccgccgcgcc ggcgacggcc acgcccaggc cctggccctc
      901 tactggctca gccgcgtggc catcagccgc ggcgactatg cccaggcgcg gatggtgctg
      961 gcctatgccc tgccgctggc ccgcgccgcc gacgccgaga cgctggccca ggtactctac
     1021 ggcgtcgccg acgtggcctg gaagaccggc gacatggaca ccctcgccaa ctacatccgc
     1081 gagagcctca acctggcccg cgccctcgac aacaccgcgc tggaactgat ggccctcaac
     1141 cgcctgggaa cgatggccta cttgcagggc gacctggaaa cggcccacac ctactatcgg
     1201 acctgcctgg aactggccca gacaaccggc aacctggagc gcgaggctac ggcgctgatg
     1261 aatctgggcg cgttggccca ccgtggcggc gaccccgacg ccgggttggc ccacttccgc
     1321 gacgcgctgg cccgctaccg cgagttgggc cgcaccgagc aggtcgtcat ggccctgggc
     1381 aacgccgccg aggccgacat cgaccgcggc gatctgggcc aggcgcgcag cgatctgggc
     1441 gagggcatgg cgttggcctg gcggctgggc ctgttgccgc gcgtcacggt attgctcttc
     1501 gtctacggcc gcctgctcat cgccgaaggg cagcacgaac gcgccgcggc cgtcctgcgc
     1561 ctggtgctcg accacccggc cacggagagc cagacccgcc cccaggcgct ggccctgctg
     1621 aatctgctgg ggcagccggg cgacgggccg gcggccgacc tggagaccat tgtggcggag
     1681 gttctcgcct gacccctgga cgagcaactc atcgacaggg ctagtcgatg gggtaatcct
     1741 gcctattgac actggcggcc cgtctacact atagtgaccc agtgtttctc gaactgccgt
     1801 cgaatagccg ccccaacggc taccgaccgc aaattaagca tacggagacg aatatgattt
     1861 ctaagcgcgc cccactgtct cgcttcgttc ctgcgcggcg tattcacgtt gccgccgtgg
     1921 ccctctttgc cgcccttgtt ctgttgctgg gccacccgcc ggcccaggcc gccccgacgt
     1981 ctccggccgt agccccaatg gtcagcccgc cgaccaacca gatcatcgtc caattcgctc
     2041 cggccgggga ggccgccctg ctctcggcca acacgggcca gttgctcgac cggctgagca
     2101 cggccgcggg cctaaccgtg acgctggagc ggccgatgtc gggcaacgcc tacgtcgtgg
     2161 gcttgcccgc ccgcatcgac gaggccgccg tggccgccat ggccgcgcgc atggccgcgc
     2221 tgccagaggt ggtctacgct gagccggacg ccatcatgca gatcatcgcc tccccgcccc
     2281 tggccgaggc ccccgccgcc aacctgacgc ccgacgacac gcgcttcgcc gaccagtggc
     2341 actaccgcta cgtgcccggc gtcgaggaag ggctgaacct gctgcccgcc tggggcatca
     2401 ccaccggctc agccgccact gtcgtcgccg tcatcgacac aggcatccgc ggccacgccg
     2461 acctggccgg ccgcaccgtg cccggctatg acttcatcgc cgacgcaccg acggccaacg
     2521 acggcaacgg ccgcgacaac gacccgaccg accccggcga ctggagtaca gccggccagt
     2581 gtttccccgg cagcacagcg cgcgacagct cctggcacgg cacacacgtc gccggcacca
     2641 tcggcgcggc gtcgaacaac ggcagcgacg tagccggcgt caattggaag gccaaaatcc
     2701 tgcccctgcg ggtgctaggg cgctgcggcg gctttctctc tgacattgtg gatgccaccc
     2761 gctgggcggc cggcctggcc gtgcccggcg cgccggccaa cgccaacccg gccgatgtca
     2821 tcaacctgag tctgggcggc agcggcgcgt gcggcgcgag ctatcagaac gccttcaacg
     2881 acctggccgc ggccggcgtt gtgtccgttg tcgccgcggg caacagcgcc atcaacgccg
     2941 ccaatgctcg cccggccaac tgtaacaatg tcatcaccgt cgccgcgacc agcaagaccg
     3001 gcgaccagac ctattacacc aacttcggcg cgctggtcga gatcgccgcg ccgggcggcg
     3061 aacagttcgc ccccaacgac gccggcggcg tcctgtccac gctcaacgcc ggtctgaccg
     3121 gccccggcgc cgacgacctg cgctactatc agggcacgag catggcctcg ccccacgtcg
     3181 ccggcctcgt ctccctgctg ctgggcgaag accccagcct gaccccggcc gaagtgctgg
     3241 acatcctgca aacgaccgcc cgtcccttcc ccgccggcag cagctgcgac acgtccacgt
     3301 gcggcgcggg cattgccgac gccttcgccg ccctcagcgc tctcaacccc ggcctgagcg
     3361 cgccccttct gatcaccccc accaacggtc agacgctggc cacggccgag gtggcgttcg
     3421 agtggggggg cgtcgaaaac gccacgggct accgcttgca ggtggccacc gatagcgact
     3481 tcaacgacct cgtcgtcaat gacgcttccc tgctcaatac ctcggccacg aagacgctac
     3541 ccggcgaagg cacctactac tggcgcgtgc gcgctctggg cgacgaggac ggcccgtgga
     3601 gcgaggtgtg gtcgttcacc atcgcgttcc cggcctgcac cgtgcccgac gcgccgatgc
     3661 tgtccgaccc ggccgatggc agcagtgtgg gcgacctgac gccgaacttc caatggggcg
     3721 cggtgatgga cgcgacgacc tacgacatcc gcgtcagcaa gcagccggac gtttccaacc
     3781 cgatcatcag cgacagcagc gccacggcgg gctacaccgt caccacttca ctgaccgaca
     3841 acgagaccta ctactggcgg gtgcgcgccc acaacaacgg cggcgcctgc aacgaaaccg
     3901 gcccgtggag cgaagtgtgg caattcagca tcgccgtatc gacctgcccg acgccggcca
     3961 tccccgagct gctcttcccc gaagacgcca gcacggtcaa cgccgcgccg acactcacgt
     4021 gggcggacgc gaccgacgcc acggaatatg aggtacgcat cggcattgag ccggatgtgg
     4081 ccgacaccct caagcaggcc acagtgaccg acacggagta cgccatcgac ctgacgttga
     4141 ccgatgggac gacatactac tggatggttc gcgccggcaa cagcgacggc tcgtgcaata
     4201 cgttgggcga ctggagcgcg gtctggagct tcacttacga aaagccccag ccggtgactt
     4261 acctggccta tctgccggcc gtctttggcc agtagaatgg tcaggccaac ggcccggccg
     4321 gccgcggacg cctgtccgct ggccggccgg accgttggca gagcctacag agccaggata
     4381 agacccgcca ctgtgaagta gataaatagc ccggtggcgt ctaccaacgt gctcatggcc
     4441 ggcccggaga cgaccgtcgg gtcgatgcgc aggcggttgg cgatgatggg caacagcgag
     4501 ccgaggacat tggcccagac gacaatggcg aagatcgaca ccgacaccgt cagcgccacc
     4561 ggctgccccg tgccccacag cagcgcccgg ccataggcga caatgcccat caccaggccc
     4621 agcaacagcc caaccgacag ctcatgcatc agcgggcgca tcaggccgcg cttgtccagg
     4681 tcttcgacgg ccagcgcgcg gataatggtg ctggtcgtct gcgagccggc gttgccgccc
     4741 gtgccgatga gcagcgggat gaagaaggcc aacgagacga cggcagccat ctccgcttcg
     4801 aagtggcgca ggacgctgcc ggtcagcgtt tcggtgacaa acagcagcaa cagccagccg
     4861 acgcgcttgc gggcgacggt caggacgctt gaatccagat agggccggtc gaggggctgc
     4921 gtaccaccga agcgctggaa gtcctcggt
//
LOCUS       DAOSMQ010000226         4948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17163_length_4948_cov_4.080932, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000226 DAOSMQ010000000
VERSION     DAOSMQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17163_length_4948_cov_4.080932"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1949
                     /locus_tag="PK829_08570"
     CDS             183..1949
                     /locus_tag="PK829_08570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOU41307.1"
                     /translation="MTQTMTPTRWSLDDLVAEPVQENIDMALATLEADVAAFEGQRDQ
                     LREDMDPAELLALLRQYESLMHHGHLLGAYAYLRFSENTQDQTSLSIRDRLDQALTAF
                     ENRLLFFTLWLKALPEEAAARLMPADPDLQYYITSLRKFQPHTLTEPEERIINLKDTN
                     GIDAMTNLYDMIVSAFSFRLEIDGETQTLTRDQLAIYFRHPSADVRAAAYQELYRVYS
                     DNSAALAQIYSHRVRDWYAESVGLRHFPTPMSVRNLQNDIPDQVVEALLEASRRNNAL
                     FQRYFRLKAEWLGLDKLRRYDIYAPLAPADRAYSYDDGLALVLDSLNAFDSRLAALVQ
                     RVIDADHVDAEPRPGKRGGAYCYSVAPELTPWVFTNYSGKARDVATVAHEMGHAIHGM
                     LAANHSPLTFHASLPLAETASVFSEILLTNRLLQNETDPAVRRDLLAYALDDAYATVQ
                     RQASFSLFEREAHALIQDGATAEQVCAAYLDNLRAQFGDAVEVSDEFRWEWISVPHFY
                     ATPFYTYAYAFGQLLVLALYEQYKQEGEAFVPRYLKLLAYGGSAEPYQVLTEAGFDVA
                     TTEFWQRGYDALEAMLDELESL"
     gene            2024..3805
                     /locus_tag="PK829_08575"
     CDS             2024..3805
                     /locus_tag="PK829_08575"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41308.1"
                     /translation="MGAKPPSPYRVFRHRDFRLMWLGQFVSTAGTALTSLAASILVYR
                     LTNSALSVGLMMMATAAPSLLVGLFAGVIVDRYDRKKIMIVTELLRAVLIALIPFLVV
                     RNIAWLYIIVALTSAIAQFYDPAHESVLPEVAPEEELAAANSLMAISSFGSTAIGFAA
                     SGLIASAANINWAFWLDAVSFLVSALCILFVSIKTLGAEEEASAAMVVRNLRVGLRFL
                     ADTPVLRSLFLVYIPVLIAFGLSNSLLLPFSDRALGANEFQYGLQESLTSVAFVACSL
                     LMAKFFDRMREGQWMALSWLVMGGLSVIYSRLTSIPLAIAIIGLSGFANAPSAIGGRL
                     ILQRNTPREMRGRVNSAFFVARDVLFLIGMAAVGFADFIDVRTMFLISGLMLAAGGAL
                     VLFLPGLRHDALAWQRSIALLRGAPAAPRLGPGRAARLGDADTLVDLIPTLCTLSPRD
                     RDTLASHAVVIGVPAGTAIVRRGEISDKAYFVLNGRAVAGYEDTDGSFRALNPLLAGD
                     FFGEIAALTGSPRTADVVASEDMELMQVPAVALRDLMSSPAFSTLVLETMTARLSVVS
                     STVMPRFAGFDQADLRDLRTNSYEGQA"
     gene            3816..4514
                     /locus_tag="PK829_08580"
     CDS             3816..4514
                     /locus_tag="PK829_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU41309.1"
                     /translation="MTALEDKRRYYLTCAPLLMGISDQQLGVLARISQWRQYDRGDFL
                     YLQGDRPVCAFVICAGWVVITLNSADGRELAISEMRDGELFGQHALIAGVPHTSSAVA
                     REATSVLAISQAAFLDVLATEPRVVRRLLEIAAGRLTEANARQSALAFLQAPARIARL
                     LREMDEKDRATADKGYITLSQEELALRAGLTRQTVARFLGEWRRNGWLLTGRGRIMLL
                     HRQALLGIEDQNAL"
     gene            4648..>4948
                     /locus_tag="PK829_08585"
     CDS             4648..>4948
                     /locus_tag="PK829_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41310.1"
                     /translation="MDEKQTAKALFKKLSALRATLSDDEQALLDALVQGEYEATAHGM
                     TAKAATGRVYQDADEAAAHGMTAKASAGRVTHDADEAAAHGMTAKAATGRVYQDAD"
BASE COUNT          844 a         1732 c         1488 g          884 t
ORIGIN      
        1 cgggctcata acccggaggt cgtcagttcg aatctgaccc ccgctactcc tagtaaaacc
       61 agagccatcc ccggctctgg ttttttattg ccgccgcgag ccggttttcc tttgcgcagc
      121 cggcgaatcg agtacaattc gccgcctaca cttggaacct ttaaccacaa acaggtgcac
      181 gtatgaccca aaccatgaca cccactcgct ggagcctgga cgatcttgtg gccgaacccg
      241 tccaggagaa tatcgacatg gcgttggcca cgctggaggc cgacgtggcc gccttcgagg
      301 gccagcgcga ccaactgcgc gaggacatgg acccagccga gcttctggcc ctgttgcgcc
      361 agtacgagtc gcttatgcac cacggccacc tgttgggcgc ttacgcctac ttgcgtttct
      421 ccgagaacac ccaggaccag acctcgctca gcatccgcga ccgacttgac caggcgctga
      481 ctgcgttcga gaaccgcttg ctcttcttca cgctgtggtt gaaggctctg cccgaagagg
      541 cggccgcccg cctgatgccg gccgatcctg acctccaata ctacatcacc tcgctacgca
      601 agttccagcc gcatactctg accgagccgg aagagcgcat catcaacctg aaggacacca
      661 acggtatcga cgcgatgacg aatctctatg acatgatcgt cagcgcgttc tccttccggc
      721 tagagatcga tggcgagacg cagacgctga cccgcgacca gctggcgatc tacttccgcc
      781 acccttcggc cgacgtccgc gccgccgcct atcaggagct ataccgcgtc tacagcgaca
      841 acagcgccgc gctggcccag atctatagcc accgcgtgcg cgactggtat gctgagagcg
      901 ttggcctgcg ccacttcccg acgcccatgt cggttcgcaa cctccaaaac gacatccccg
      961 accaggtggt cgaggccctg ctagaggcca gccggcgcaa caacgccctg ttccagcgtt
     1021 acttccgcct gaaggccgag tggctgggcc tggacaagct gcggcgctat gacatctacg
     1081 cgccgctggc cccggccgac cgcgcctaca gctacgatga cggtctggct ctcgtgctcg
     1141 acagcctcaa tgccttcgac tcgcggctgg ccgcgctggt gcaacgggtc atcgacgccg
     1201 accacgtcga cgccgaacca cggccaggca agcgcggcgg cgcttactgc tactccgtgg
     1261 ccccggaact aacgccgtgg gtcttcacca actactccgg caaagcccgc gacgtggcca
     1321 ctgtggccca cgagatgggc cacgccatcc acggtatgct ggccgccaac cattcgccgc
     1381 tgacgttcca cgcctcgctg ccgttggccg agacggcctc agtcttcagc gagatcttgt
     1441 tgaccaaccg cctgctgcaa aacgagacgg acccggctgt gcgccgagac ctgctggcct
     1501 acgccctcga cgacgcctac gccaccgtgc agcgccaggc cagcttcagc ctgttcgagc
     1561 gcgaggccca tgccctcatc caggacggag ccacagccga gcaggtctgc gcggcctatt
     1621 tggacaactt gcgcgcccag ttcggcgacg ctgtggaggt cagcgacgaa ttccgctggg
     1681 agtggatcag cgtgccccac ttctacgcca cgccgttcta cacctacgcc tatgccttcg
     1741 gccagttgct cgtcctggcg ctctatgagc agtataagca ggagggcgag gcgttcgtgc
     1801 cgcgctacct gaagctgctg gcctatggcg gctcagccga gccgtaccag gtgctgaccg
     1861 aggccggttt cgacgtggct acgaccgagt tctggcaacg cggctacgac gccctggagg
     1921 ctatgctgga cgagctagaa agtctatagc gccacccacc gctgcgttat cgttatgacg
     1981 accaaaaagg agggcaacag ccataaccac cgccacagca gccatcggcg ctaaaccgcc
     2041 gtcaccctat cgcgtctttc gtcatcgcga cttccggctg atgtggctgg gccagttcgt
     2101 gtccacggcg ggcacggcgc tgacctcgct ggccgcgtcg atcctggtct accggctgac
     2161 caactcggcc ctcagcgtcg gcctgatgat gatggccacg gccgcgccga gcctgctggt
     2221 gggcctcttc gccggagtca tcgtcgaccg ctacgaccgc aagaagatca tgatcgtcac
     2281 cgagctgctg cgcgcggtgt tgatcgccct catccccttc ctggtggttc gcaacatcgc
     2341 ctggctctac atcattgtcg ccctgaccag cgccatcgcc cagttctatg acccggccca
     2401 cgagagcgtc ctgcccgaag tggccccgga agaagaactg gccgcagcca attcgctcat
     2461 ggccatcagc tccttcggct ccacggccat tggcttcgcg gcgtcgggac tgatcgcctc
     2521 ggcggccaac atcaattggg ccttctggct cgatgccgtc tccttcctcg tctccgcgct
     2581 ctgcatcctg ttcgtgtcca ttaagacgct gggcgcggag gaagaggcca gcgcggccat
     2641 ggtcgttcgc aacctgcgcg tcggtctgcg cttcctggcc gacacgcccg tcctgcgctc
     2701 gctctttctg gtctacatcc cggtgctcat tgccttcggt ctgtcgaact cgctgctgtt
     2761 gcccttctct gaccgggcgc tgggggcgaa tgagttccag tatggtttgc aggagagcct
     2821 gacctcggtg gccttcgtcg cctgtagtct gctaatggcc aagttcttcg accggatgcg
     2881 cgagggacag tggatggcgc tgagctggct ggtgatgggc gggctatcgg tcatttactc
     2941 gcggctgact tctatcccgc tggccattgc catcatcggc ctgtccggct tcgccaacgc
     3001 gccatcggcc atcggcggcc ggcttatcct ccagcgcaac accccccgcg agatgcgcgg
     3061 ccgggtcaat agcgccttct tcgtcgcccg tgacgtcctg tttctgatcg gcatggccgc
     3121 ggtgggcttc gccgacttca tcgacgtgcg gacgatgttc ctcatcagcg gcctgatgct
     3181 tgccgccggc ggcgcgctgg tgctgttttt gcccggtcta cgccacgacg ctctggcctg
     3241 gcaacgctcc atcgccctgc tgcgcggcgc gccggccgca ccacggcttg gcccggggcg
     3301 ggccgcccgt ctgggcgacg ccgacacact cgttgacctg atcccgacgc tgtgcacgct
     3361 gtcgccacga gatcgtgaca cgctggcttc ccacgcggtc gtcatcggcg tgcccgccgg
     3421 cacggccatc gtccgccgcg gcgagataag cgataaggcc tacttcgtcc tcaacgggcg
     3481 tgccgtcgct ggctacgagg acaccgacgg tagttttcgc gctctgaacc cgttgctggc
     3541 cggcgacttc ttcggcgaga ttgccgccct gaccggctcg ccgcgcacgg ccgacgtcgt
     3601 cgccagcgaa gacatggaac tgatgcaggt gccagccgtc gcgctacgcg atctgatgag
     3661 cagcccggcg ttcagcacgc tcgtcctgga gacgatgacc gcgcggctga gcgtcgtcag
     3721 cagcacggtg atgccacgct tcgccggctt cgaccaggcc gacctgcgcg acctgcgcac
     3781 caattcttac gagggccagg catagctttg agacgatgac cgccctggaa gataaacgcc
     3841 gctactatct cacctgcgcg ccgctgctga tgggcatcag tgatcagcag ttgggagtct
     3901 tggcgcgcat cagccaatgg cggcaatacg accgcggcga cttcctgtat ctccagggcg
     3961 atcgccccgt ctgtgccttc gtcatttgcg ccggctgggt cgtcatcacc ctcaacagcg
     4021 ctgacggccg cgaactggcc atcagcgaaa tgcgcgatgg cgagctgttc ggccagcacg
     4081 cgctcatcgc cggcgtgccc cacacctcca gcgccgtcgc ccgcgaggca acgagcgtgt
     4141 tagcgatttc gcaggcggcc ttcctggacg tgctggccac ggagccgcgt gtcgtgcgcc
     4201 ggctgctgga gatcgccgct ggccggctga ccgaggccaa tgcccgccag agcgccctgg
     4261 ccttcctgca agcgccggcc cgcatcgctc gcctcctgcg cgaaatggac gagaaggacc
     4321 gcgccacggc cgataagggc tatatcaccc tttcacagga ggagttggcc ctgcgcgcgg
     4381 gcctgacgcg ccagacggtg gcccgcttcc tgggcgagtg gcggcgcaac ggctggctgc
     4441 tgaccgggcg cgggcgaatc atgctcctcc atcgccaggc cctgctgggc atcgaggatc
     4501 aaaacgcgct gtagaaccaa cggcgcggcc tatgtcgccc agacgacaga cgcccgaccg
     4561 ggttgcctgc tatagtggcg gcacgttgaa aatcaaatca tggtcatcct agaggaacgg
     4621 ccaggcagaa cgataggaga cagagagatg gacgagaagc agacagcaaa ggcattgttc
     4681 aagaagttgt cggccctgcg ggccacgctg agtgatgacg agcaggcgct cctggacgcg
     4741 ctcgtccagg gcgagtacga agccaccgcc cacggcatga ccgccaaagc tgccacgggc
     4801 cgcgtctatc aggacgccga cgaagccgcc gcccacggca tgaccgccaa ggcctcggcc
     4861 ggccgcgtca cccacgacgc cgacgaagcc gctgcccacg gcatgaccgc caaagctgcc
     4921 acgggccgcg tctatcagga cgccgacg
//
LOCUS       DAOSMQ010000227         4945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17174_length_4945_cov_4.139264, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000227 DAOSMQ010000000
VERSION     DAOSMQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17174_length_4945_cov_4.139264"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PK829_08590"
     CDS             complement(<1..540)
                     /locus_tag="PK829_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41311.1"
                     /translation="MAYGMLKRSSFPPRRVALRAGLCLAVVAALVLSIRPPAPAVIAF
                     GPRQEVHTTTPILGVHTRLTDEVEPWKIQYTLRLVRQMGAPWIVEFFPWAYYQAEDGS
                     VAWDHPDQVVGHAEANGLRVVARLGYTPEWARPADTPLTYLDADAYDDFAAFAAAFAA
                     RYRGRVAALIVGNEPNLSFE"
     gene            776..1741
                     /locus_tag="PK829_08595"
     CDS             776..1741
                     /locus_tag="PK829_08595"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU41312.1"
                     /translation="MRVFITGATGFAGSHLVERLLADGHEVTALVHAATSHQELPDHP
                     RIRPVASDLLDPAAVRDALAKARPDVVFHLAGQAYPARSWDEPALTLAVNAGGTANIL
                     RAATEFGRPRVVIVTSAEIYGVLGREAMPLTEETVARPRHPYGVSKLAAGELARVYWE
                     RYGLPVVEARPFNHIGPRQARGFVVPDFAAQLAAIRLGRQEPVMRVGNLDPERDFTDV
                     RDVAAAYLRLAEAGRPGEAYLICSGRSVSVRNLLETLIDLAGVSVDVQTDAARLNPSD
                     TPCLYGSNAKIAADTGWRPTISLRQSLADALADWEARLLAEAAPA"
     gene            1755..2531
                     /locus_tag="PK829_08600"
     CDS             1755..2531
                     /locus_tag="PK829_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOU41313.1"
                     /translation="MSKGKERLDKLLVDRGLMATRARARAAIMAGEVRVNGERVDKAG
                     TPVPLDATIDVAAPMPYVSRGGFKLAGALDAFGVVAVGRVCADVGACTGGFTDVLLQR
                     GAARVFAIDVGQGQLEWKLRQDPRVVVLERTNARYLESLPEQVDLVVIDVSFISLRLI
                     LPAVARWLAPAADVVALIKPQFEAGPESVGKGGIVRDPAVRRRVLEDVLGWAATNGWG
                     VAGLTPSSIQGADGNVEYLAWLRPSGTPTDLTRLIDDLFN"
     gene            complement(2583..3020)
                     /locus_tag="PK829_08605"
     CDS             complement(2583..3020)
                     /locus_tag="PK829_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU41314.1"
                     /translation="MSHDPNPTPPTPDPTFHVVTPADRDPVTVQELRRLPVRELPQSF
                     IVSTGHGTSGPFAFAGVTLRDVIAAYAPGEWAAAEVVSADGFATRVTAGEWLAETARP
                     LLLAYEIDGRPMSREEGLVRLIVPTETDEALRQVKWVAEIRLS"
     gene            3077..4882
                     /gene="lepA"
                     /locus_tag="PK829_08610"
     CDS             3077..4882
                     /gene="lepA"
                     /locus_tag="PK829_08610"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU41315.1"
                     /translation="MEQSRIRNFCIIAHIDHGKSTLADRLLQATGTISDREMQEQVLD
                     TMDIEREKGVTIKASAVRMNYRARDGETYEMNLIDTPGHVDFAYEVSRALQSCEGAIL
                     VVDATQGIEAQTLANLYMAVEADLELLPVVNKIDLPAASPEDVAEEIEHVTGFPAEEV
                     IPISAKMGTNIDAVLEAVVRKIPPPAGDRDAPLRALVFDSHYDSYKGVIAYVRVFEGS
                     VRHRQAVKMMSNDVSVEPIEIGIFSPGMVPAEELGAGEVGYIATGLKTVRECRVGDTI
                     TLRDKPAAEPLHGYKTVKPMVYAGLYPSEGEDYPLLKEALERLQLNDASLVFEPETSN
                     ALNFGFRCGFLGLFHMEIIQERLEREYDLDLVATAPSVEYEVVMLNTGEVRFIESPAD
                     LPDESTIAEIREPWMHVQIFAPEEFYGVVMDLARKRRGEFIKQEHPAPGRVVLHYYLP
                     LAEMIVDFYDKLKSSTRGYASIDYEFDGYRASDLVKLEVLVAKQPVDALAMIVHADDA
                     YHRGQRLVSELKDLIPRQLFEVPIQAATSKRVISRANVKALRKDVLAKCYGGDITRKK
                     KLLEKQKKGKKRMKMIGNVEVPQEAFMAVLKLGED"
BASE COUNT          853 a         1636 c         1663 g          793 t
ORIGIN      
        1 ctcgaagctc aggtttggct cgttgccgac gatgagggcc gccacccggc cacggtagcg
       61 ggcggcgaag gcagcggcga aggcggcgaa gtcatcgtag gcatcggcgt caaggtaagt
      121 caggggcgta tccgccgggc gcgcccactc cggcgtgtag cccagccggg cgacgacccg
      181 cagcccattg gcctcggcgt ggccgacgac ctgatcgggg tggtcccagg cgacgctgcc
      241 gtcctcggcc tggtagtagg cccaggggaa gaactcgacg atccacggcg cgcccatctg
      301 gcgcaccagc cgcagggtgt actggatctt ccacggctcc acctcgtcgg tcaggcgcgt
      361 gtggacgccc agaatggggg tagtagtatg cacctcttgc cgcgggccga aggcgatcac
      421 tgccggcgcg ggcggccgga tgctcaacac cagcgcggcc acaaccgcca gacacaaccc
      481 ggcccgcaaa gccacgcgcc ggggaggaaa agatgagcgc ttcaacatgc cgtaagccat
      541 tatagagccg ttcggccgcc ctccaagcgc gttgcccggc gactcataat cgggatcagg
      601 gcttggccaa agtcgtctcg cgcccattga cctgacaggt gggcgcccac ctgtcaggtc
      661 ggcgcgcctg ggatgacttt ggcaaagccc tgtaatcggg atatggccat tcggccaagg
      721 gggctataat gggccaatca cctccgcgtc cgcgacgcgg agcctgggag tggctatgcg
      781 cgtatttatc accggggcga ccgggtttgc cggttcccat ctcgtcgagc gattgctggc
      841 cgatggccat gaggtgaccg cgctcgtcca cgccgccacc agccaccagg aattgcccga
      901 ccacccgcgc atccggcccg tggccagcga tttgctcgac ccggcagcgg tgcgcgacgc
      961 gctggccaag gcgcggccgg atgtcgtctt ccacctggcc gggcaagcct acccggcgcg
     1021 ctcgtgggac gagccggcgc tgacgctggc cgtcaacgcc ggcggcacgg ccaacatcct
     1081 ccgggcggcg accgagtttg gccggccgcg cgtggtcatc gtcaccagcg cggagatcta
     1141 cggcgtgctg gggcgcgagg ctatgccgct gaccgaggag accgtcgccc ggccgcgtca
     1201 tccctacggc gtcagcaagc tggccgcggg ggaactggcc cgcgtctact gggagcgcta
     1261 cgggctgccg gtcgtcgaag cgcggccgtt caaccacatc ggcccccggc aggcgcgtgg
     1321 cttcgttgtg cccgatttcg ccgcccagct ggcagccatc cgcctgggcc ggcaggagcc
     1381 agtcatgcgc gtcggcaacc tggacccgga gcgcgacttc accgacgtgc gcgatgtggc
     1441 cgccgcctac ctgcgcctgg ccgaggccgg gcggccgggc gaggcctacc tcatctgctc
     1501 cggtcgctcc gtcagcgtgc gtaatctgct ggagacgctc atcgacctgg ccggcgtgag
     1561 cgtggacgtg cagaccgacg ccgcccgcct caacccgtcg gacacgccct gcctctacgg
     1621 cagcaacgcc aagatcgccg ccgacacggg ctggcggccg accatctctc tgcgccaatc
     1681 gctggccgac gcgctggccg actgggaggc gcgcctgctg gccgaggccg cgccggcctg
     1741 atccggcaac accaatgagc aagggcaagg aacggctcga caagctgctc gttgaccgcg
     1801 gcctgatggc gacgcgggcg cgggcgcggg cggcgatcat ggccggcgag gtgcgcgtca
     1861 acggcgagcg cgtcgataag gccggcacgc ccgtgcctct cgacgcgact atcgacgtgg
     1921 ccgcacccat gccttacgtc agccgcggcg gtttcaagct ggccggggcg ctcgatgcct
     1981 ttggcgtggt tgcggtcggg cgcgtctgcg ccgatgtggg cgcgtgcacc ggcggcttca
     2041 ccgatgtact cctgcaacgc ggcgcggcgc gcgtcttcgc catcgacgtc ggccagggac
     2101 aattggagtg gaagctgcgc caggacccgc gcgtcgtcgt gctggagcga acgaacgccc
     2161 gctatctgga gtcgctgccg gagcaggtcg atctggtggt catcgacgtg tccttcatct
     2221 ccctgcgcct catcctgccg gccgtcgccc gctggctggc cccggcggcc gacgtcgtcg
     2281 ccctcatcaa gccgcagttc gaggccgggc cggagtcggt cggcaagggc ggcatcgtcc
     2341 gcgatccggc cgtgcggcgg cgggtgctgg aagatgttct cggctgggcg gcgacgaacg
     2401 gctggggcgt ggccgggctg acgccttcct ccatccaggg cgcggatggt aacgtggagt
     2461 atttggcctg gttgcgacca tccggcacgc caacggactt gactagactc atagatgatt
     2521 tgttcaattg agttgaggaa gttagtggat ttccctctga agaggcgggt tgccaacccg
     2581 ccctacgaca agcgaatctc agccacccac ttgacctgcc gcagggcctc gtccgtctcc
     2641 gtgggcacaa tgaggcgcac taggccctct tcccggctca tgggccggcc atcgatctca
     2701 taggccagca ggagcggccg cgccgtctcg gccagccact cgccggcggt cacgcgcgtg
     2761 gcgaagccgt cggcgctgac gacctcggcc gcggcccact cccccggcgc gtacgcggcg
     2821 atcacgtcgc gcagcgtgac gccggcgaag gcgaacggcc cgcttgtgcc gtggccggtg
     2881 ctgacgatga agctctgcgg cagctcgcgc acgggcagcc ggcgcaattc ctgcaccgtc
     2941 acggggtcgc ggtcggccgg cgtgacgacg tggaaggtgg gatcgggtgt cggtggggtg
     3001 gggttgggat catggctcat gggcgttctc gcgggcagca ctgtctgcgt ccgcccattc
     3061 tgctatactt gcgcccatgg agcaaagtcg aatccgtaat ttctgtatca tcgcccacat
     3121 cgaccacggc aaatccaccc tggccgaccg gcttctgcaa gcgaccggca ccatttccga
     3181 ccgcgaaatg caggagcaag tgctcgacac catggacatc gagcgcgaga aaggcgtcac
     3241 catcaaggcc tcggccgtgc gcatgaacta ccgcgcccgc gacggtgaga cctacgagat
     3301 gaaccttatc gacacgcccg gccacgtgga cttcgcctac gaggtcagcc gggcgctgca
     3361 aagctgcgag ggggccatcc ttgtcgttga cgccacccag ggcattgagg cgcagacgtt
     3421 ggccaatctc tatatggccg tcgaggccga cctggagttg ctgccggtcg tcaacaagat
     3481 cgatctaccc gccgcttccc cggaggatgt ggccgaggag attgagcacg tcaccggctt
     3541 tccggctgag gaagtcattc ccatcagcgc caagatgggc acgaacatcg acgccgtgct
     3601 ggaggccgtc gtccgcaaga tcccgccgcc ggcgggcgac cgtgacgcgc ccttgcgcgc
     3661 cctcgtcttc gactcccact acgacagcta caagggcgtc atcgcctacg tgcgcgtctt
     3721 cgagggttcg gtgcgccacc gtcaggccgt caaaatgatg tccaatgatg tcagcgtcga
     3781 gccaatcgag atcggcatct tcagccccgg catggttccg gccgaggaac tgggcgcggg
     3841 cgaggtcggc tacatcgcca ccggcctgaa gacggtgcgc gagtgccgcg tcggcgacac
     3901 cattaccctg cgcgacaagc cggcggccga gccgctgcac ggctataaga cggtcaagcc
     3961 gatggtctac gccggactgt accccagcga aggcgaggac tacccgctgc tgaaggaggc
     4021 tctggagcgg ctccagctca acgacgcctc cctcgtcttt gagccggaga cctccaacgc
     4081 gctcaacttc ggcttccgct gcggcttcct gggcctgttc cacatggaga tcatccagga
     4141 gcggctggag cgcgagtatg atctcgacct ggtagccacc gcgccgagcg tggagtatga
     4201 agtcgtcatg ctcaacaccg gcgaggtgcg cttcatcgaa agcccggccg acctgcccga
     4261 cgagtcgacc atcgccgaaa tccgcgagcc gtggatgcac gtgcaaatct tcgccccgga
     4321 ggagttttac ggcgtggtca tggacctggc ccgcaagcgg cgcggcgagt tcatcaagca
     4381 ggagcatccc gcacccggcc gcgtggtgct acactattat ctgccgctgg cggagatgat
     4441 cgtcgatttc tacgacaagc tgaagagcag cacgcgcggt tatgcctcga ttgactacga
     4501 gttcgacggc taccgcgcct ccgatctggt caagctggag gtgttggtgg ccaagcagcc
     4561 ggtggacgcg ctggcgatga tcgttcacgc cgacgacgcc taccaccgcg gccaacgcct
     4621 cgtctccgaa ctgaaagacc tcatccctcg ccaactcttt gaagtgccca tccaggcggc
     4681 gacgagcaag cgcgtcatca gccgggccaa cgttaaggca ctacgcaagg atgtgctggc
     4741 aaaatgctat ggaggcgaca ttacccggaa gaaaaagctg ctggagaagc agaagaaggg
     4801 taagaagcgc atgaagatga tcggcaacgt cgaggtgccg caggaggcat ttatggcggt
     4861 gctgaagttg ggcgaagact aaggctgaag gatgaaagag gaaggatgaa atatgaaggc
     4921 tgaaggatga aggttgcctt cagcc
//
LOCUS       DAOSMQ010000228         4938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17202_length_4938_cov_4.184723, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000228 DAOSMQ010000000
VERSION     DAOSMQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17202_length_4938_cov_4.184723"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /gene="feoB"
                     /locus_tag="PK829_08615"
     CDS             <1..1848
                     /gene="feoB"
                     /locus_tag="PK829_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOU41316.1"
                     /translation="MIDVAMRQGLDFDVARLSEALGAAVVPMAARRGEGLEHAVAAVT
                     AAAVVPRSPFRLDYGPAVEAEIDRLVATLAQLPLTCPARWLAIKLLEDDADMATKVAA
                     AAGGAKVLATAADARGQLEAALGEDADTVMADRRYAWIHALVGQAMHAERADVLTLSD
                     RIDRFATHRLLGIPLFLAAMWLVFKVTTDLSAPWVDWLDAVLTGPLSRWMLAALGALG
                     LGQTWVASMAVDGVLAGVGSVLAFVPVLLALYFALAVLEDSGYMARGAFVMDRLMKRI
                     GLQGRSFLPLMVGFGCSVPAIYATRTLTNQRDRVLTGLLVPFISCGARLPVYVLFAAI
                     FFPARAGLVILGLYVLGIVVALGVGLLLQRTLLPATVEAGLVMELPPYRLPNARSIGF
                     HMWQRTRAFLQHAASLILMMTLAIWLLTAIPLGGAGTFANTPMSASAFGRVSALAAPA
                     LRPLGFGSWESGGALLTGLVAKEVVVSTLAQIHDVAPAPDGAEATTFAGDLVAIVTSF
                     GRAVVETLRAIPGLVGINLTAGQTGDDAALSTALRASFEESSGGHAAAAGLAFMVFVL
                     LYTPCMAAVAAERHELGARWMWLSLFGQLAIAWLAALTVFQLGRLWGGG"
     gene            1848..2129
                     /locus_tag="PK829_08620"
     CDS             1848..2129
                     /locus_tag="PK829_08620"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HOU41317.1"
                     /translation="MLRELLRLVEAADGPITLAELGRELNVDVAVLDGMLQHWVRKGR
                     LVTDSGTAKSCAGGCGCMSVGGGCGSCAGMAACPFVARLPVVYRLKLDE"
     gene            2122..>2735
                     /locus_tag="PK829_08625"
     CDS             2122..>2735
                     /locus_tag="PK829_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HOU41318.1"
                     /translation="MSNSIPSARAPGDTGRALVRSVALPAEKGSYSLVSEPIVLGLVA
                     APSWAGLALAVAAVGVFLFNRPLKVTLTDRRRGRSYARTALARRFLLIYAAVAAAGFA
                     VALVLGGWRPFVPLVVAAPLLGLFAVYDQRPGRSWQAELAAPVAFAAIVAAIAVADGW
                     AWTPALALWGFMVARAVPAVLFIRARLRLDKGRPAAPGEGTPAV"
     assembly_gap    2736..2835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2836..3101
                     /locus_tag="PK829_08630"
     CDS             <2836..3101
                     /locus_tag="PK829_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41319.1"
                     /translation="RLDKGRPAAPGEGTPAVILSHVAALLAVAALVWAAWLPWTAVLA
                     VGILLARAAWGLSPWRGSFSAVVLGLLETGFGLLAVLLVALAY"
     gene            complement(3066..4460)
                     /gene="argH"
                     /locus_tag="PK829_08635"
     CDS             complement(3066..4460)
                     /gene="argH"
                     /locus_tag="PK829_08635"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOU41320.1"
                     /translation="MTKLWGGRFAGQTDALVHRYNASIGFDIRLYDEDISGSVAWARG
                     LADAGLLTAEEHDTLLLGLEAVRAEFAAGTFVVALEDEDIHSAVERRLTELAGPVGGK
                     LHTGRSRNDQVATDFRLWIMRASDRLTEAVAETQRALIDSAAAAIDLPLPGYTHLQHA
                     QPVTWSHWALSHFWPLARDRARLAAVRRSAAALPLGAAALAGTAFPIDRTVLATRLGF
                     DAIAPNSLDAVSDRDFAAEFLFAAALLGVHLSRLSEQLILFSSAEFGFVRLDDAYSTG
                     SSIMPQKKNPDTLELTRGKSGRLIGHLTGFLATLKGLPSAYDKDLQEDKEPVFDAADT
                     LELALPVMAGLLRTMTLRPERMAAQLEAGLLATELADYLVRRGLPFREAHHLVGQVVR
                     RAEERGVSLTELETDELAAISPHFGPDTAGVLTVPAALAARNVPGGTAVAAVQAQLAA
                     ARRLVGQGDEQDSQ"
     gene            complement(4556..>4938)
                     /locus_tag="PK829_08640"
     CDS             complement(4556..>4938)
                     /locus_tag="PK829_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOU41321.1"
                     /translation="TVRVTGAASAADARRVADTIATSPLVKTAFAGGDPNWGRILAAA
                     GRSGAAIEPERLALWVGAPGAEPLQLVHAGTPTAFGEADAAAVFARPEFTAHVDLGLG
                     DGEATLWTTDLTHEYVTINADYRT"
BASE COUNT          591 a         1645 c         1763 g          839 t
ORIGIN      
        1 atgatcgacg tggccatgcg gcaggggctg gacttcgacg tggcccggct gagcgaggcg
       61 ctgggcgcgg ctgtggtgcc tatggccgcc cggcgcggcg aagggctgga gcatgccgtc
      121 gccgccgtca ccgcggccgc cgtcgtgccc cggtcgccgt ttcgtctcga ttatggcccg
      181 gccgtcgaag ccgagatcga caggcttgtg gccacgctgg cgcagttgcc gctgacctgc
      241 ccggcccgct ggctggccat caagttgctt gaggatgatg ccgatatggc caccaaggtg
      301 gccgcggccg ccggcggagc aaaggtgctt gccacggcgg cagacgcgcg cggccagctg
      361 gaagccgcgt tgggcgagga cgccgacacc gtgatggccg accgccgcta cgcctggatt
      421 cacgctctcg tcggccaggc catgcacgcc gaacgtgcgg acgtgctgac cctttccgac
      481 cgcatcgacc gcttcgccac ccaccgcctg ctgggcatcc cgctcttcct ggcggccatg
      541 tggctggtct tcaaggtgac gaccgacctt tccgcgccct gggtcgattg gctcgacgca
      601 gtgctgaccg gcccgctcag tcgctggatg ctggcggcgt tgggcgcgct ggggctgggg
      661 cagacgtggg tcgcctcgat ggccgttgat ggggtgttgg ccggcgtcgg cagcgtattg
      721 gcctttgtgc ccgtgctgct ggcgctctac ttcgccctgg ccgtgctgga agattcgggc
      781 tacatggccc gcggggcgtt tgtcatggat cgcctcatga agcgcattgg cttgcagggg
      841 cgcagcttcc tgccgctgat ggtcggcttc ggctgctccg tgccggccat ctatgccaca
      901 cgcacgctga ccaatcagcg cgaccgcgtg ctgaccgggc tgctggtgcc gttcattagt
      961 tgcggggcgc ggctgccggt ctacgtcctc ttcgcggcta tcttctttcc cgcgcgcgcc
     1021 gggctagtta tcctgggcct ctacgtgctg ggtattgtcg tagctctggg cgtgggtttg
     1081 ctgttacagc gcacgctgct gccggcgacg gtggaggctg gtctggtgat ggaactgcca
     1141 ccctaccggc tgcccaacgc ccgcagcatc ggcttccata tgtggcagcg tacgcgcgct
     1201 ttcctgcaac acgccgcctc gcttatcctg atgatgacgc tggccatctg gctgctgacg
     1261 gccattccgc tcggcggcgc gggcacgttt gccaacacgc ccatgtccgc aagcgccttc
     1321 ggccgtgtct cggcgctggc cgcgccggcg ctacggccgc tgggctttgg ctcgtgggag
     1381 tccggcgggg cgctattgac cgggttagta gccaaggaag tcgtcgtcag cacgctggcg
     1441 caaatccacg atgtggcacc cgcccctgac ggggccgagg cgacgacctt cgccggcgac
     1501 ctggtcgcaa tcgtcacgtc gttcggccgc gctgtcgtcg agacgctgcg ggccattccc
     1561 ggcctggtcg gcatcaacct gacggccggc caaaccgggg acgacgccgc gctttcgacc
     1621 gctctgcgcg ccagtttcga ggaaagcagc ggcggtcacg ccgccgccgc cggactggct
     1681 ttcatggtct ttgtcttgct ctacacgccg tgcatggccg ctgtggccgc cgagcgccac
     1741 gaactgggcg cgcgctggat gtggctgagc ctgttcgggc agctggccat tgcctggctg
     1801 gcggctctga cggtcttcca actcggccgc ctgtggggag ggggatgatg ctgcgtgaat
     1861 tgctacgcct ggtggaggcc gccgacggcc ccatcaccct ggccgagctg ggccgggagt
     1921 tgaacgtaga cgtggccgtg cttgacggca tgctccagca ctgggttcgc aaggggcggc
     1981 tggtcaccga cagcggcacg gctaagtcct gcgccggtgg atgcgggtgt atgtccgttg
     2041 gtgggggctg tggctcctgc gccggcatgg ctgcctgccc gtttgtcgcc cgtctgcccg
     2101 tggtctaccg tcttaagctt gatgagtaac tccattccat cggcgcgcgc gcccggcgac
     2161 actggccgcg cgttggtccg cagtgtcgct ctgccggccg agaagggcag ctatagcctg
     2221 gtgtcggagc cgattgtgct tggcctggtg gccgcgccat cgtgggccgg gctggcgctg
     2281 gccgtcgccg ccgtgggcgt gttcctgttc aaccggccgc tgaaggtcac tctgaccgac
     2341 cgccggcgcg ggcgcagcta cgcgcgcacg gctctggcgc ggcgctttct gctgatctac
     2401 gccgctgtag ccgccgccgg cttcgcggtc gccctggtgt tgggtggctg gcgaccattc
     2461 gtgcccctgg tcgtggccgc gccgctgctg ggcctgttcg ccgtctatga ccagcggccg
     2521 ggccgctcgt ggcaggcgga gttggccgcg ccggtggcct tcgccgccat cgttgccgcc
     2581 attgctgtgg ccgacggctg ggcgtggacg ccggcgctgg ccctgtgggg tttcatggtg
     2641 gcccgggcag tgcccgcggt gctgttcatc cgcgcgcgat tgcggctgga caagggccgc
     2701 ccggccgcgc cgggggaggg cacgccggcg gtcatnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnntgcgg ctggacaagg gccgcccggc cgcgccgggg gagggcacgc
     2881 cggcggtcat tctcagccac gtagcagctt tgctggcggt ggcggcgctg gtatgggcgg
     2941 cgtggctgcc gtggacggcc gtgttggccg tgggcatcct gctggcgcgg gcggcgtggg
     3001 ggctgtcgcc ctggcggggc agcttctcgg ccgtcgtcct cggccttctg gagacgggct
     3061 tcggcctact ggctgtcctg ctcgtcgccc tggcctacta accggcgcgc cgcagccaac
     3121 tgcgcctgca ccgccgcgac ggccgtgccg ccgggcacgt tgcgggcggc cagcgctgcc
     3181 ggaaccgtca gcacaccggc cgtgtctggc ccgaagtggg ggctgatggc cgccaattca
     3241 tcggtttcca actccgtcag agagacaccg cgctcctcgg cccggcgcac gacctggccg
     3301 acgagatgat gggcctcgcg aaagggcagg ccgcgccgca ccaggtagtc ggctaactcc
     3361 gtcgccagca gcccggcctc caactgcgcc gccatgcgct ccggccgcag cgtcatcgtc
     3421 cgcagcagcc cggccatcac cggcagggcc aactccagcg tgtcggcggc gtcgaaaacc
     3481 ggctccttgt cctcctgcaa gtccttgtca taggccgacg gcagtccctt cagcgtcgcc
     3541 aggaagccgg tcaggtggcc gataagccgg ccgctcttgc cgcgcgtcag ttccagcgtg
     3601 tcggggttct tcttctgcgg catgatgcta ctgccggtgc tataggcatc gtccaggcgg
     3661 acgaagccga actcggcgct gctgaagagg atgagctgct cggacaggcg gctgaggtgg
     3721 acgcccagaa gggccgcggc gaagaggaat tcggcggcga aatcgcggtc gctgacggca
     3781 tcgaggctgt tgggggcgat agcgtcgaag cccagccgtg ttgccaacac cgtccggtcg
     3841 atggggaagg ccgtcccggc cagcgccgcc gcgcccagcg gcagggccgc cgccgaccgc
     3901 cgcaccgccg ccagccgcgc ccggtcgcgc gccagcggcc agaaatggga cagcgcccag
     3961 tggctccacg tcaccggctg ggcgtgttgc aggtgggtgt agccgggcag gggcaggtcg
     4021 atagccgcgg ccgcgctgtc gatcagggcg cgctgcgtct cggccacggc ctcggtcagg
     4081 cggtcactgg cgcgcatgat ccacaggcgg aagtcggtcg ccacctggtc gttgcggctg
     4141 cggccggtgt ggagcttgcc gccgacgggg ccggccaact cggtcagccg ccgctcaacg
     4201 gctgagtgga tatcctcgtc ttccagggcg acgacgaacg ttcccgcggc gaactccgcc
     4261 cgcaccgctt ccagtcccag cagcagcgta tcgtgttctt cggccgtcag cagcccggca
     4321 tcggccaacc cccgcgccca ggcgacgctg ccgctgatgt cctcgtcgta gaggcggatg
     4381 tcgaagccga tgctggcgtt atagcggtgg acgagggcgt cggtctggcc ggcgaagcgg
     4441 ccgccccaga gctttgtcat gatgggttcc cctgcatgtg cggggagccg gggagcgggg
     4501 gagcaggggg gatagaactc gtccctgcgc ccctgcaccc cagctccccg gtctcttaag
     4561 tgcgatagtc ggcgttgatg gtgacgtact cgtgggtcag gtcggtcgtc cacaaggtgg
     4621 cctcgccgtc gccgagaccc aggtccacgt gcgcagtgaa ctccggccgg gcaaagaccg
     4681 ccgcagcgtc ggcttcgccg aaggcggtgg gcgtgccggc gtgaaccaat tgcagcggct
     4741 ccgcgcccgg cgcgcccacc cacagggcca ggcgctccgg ctcgatggcc gcgccactgc
     4801 gccccgccgc cgccaggatg cggccccaat tggggtcgcc gccggcgaag gcggtcttaa
     4861 ccaggggcga ggtggcgatg gtatcggcca cgcggcgggc gtcggcggct gaggccgcgc
     4921 cggtgacgcg cacggtga
//
LOCUS       DAOSMQ010000229         4901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17374_length_4901_cov_4.159513, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000229 DAOSMQ010000000
VERSION     DAOSMQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17374_length_4901_cov_4.159513"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..2236
                     /gene="glgX"
                     /locus_tag="PK829_08645"
     CDS             116..2236
                     /gene="glgX"
                     /locus_tag="PK829_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019507604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOU41322.1"
                     /translation="MINRIDVYPTHRHGEFWLRAGRPYPFGSTIVPGGVNFAVFSRHA
                     TACALVLYRRGEAEAMVEIPFLEAFRIGNVWAMVVFGLDIEELEYGYRMDGPHRPHAG
                     HRFDPSRVLLDPYARAISGRLRWAEAHEPADALPFRGRILLDDFDWQDDRQLELPSED
                     LIIYEMHVRGFTRHPSSGVKFPGTFAAIREKIPYLVELGINAVELMPVQEFDELENDF
                     TNPYTGQKLRNYWGYSTVGFFAPKAGYAATGGLGMEADELKALVKLLHRHGIEIILDV
                     VFNHTAEGNEKGPTISFRGLDNRVYYMLQPDGSYQNFSGTGNTLNCNNPVVRNLVLDA
                     LRYWVSEYHIDGFRFDLASILGRDAEGHPLANPPLLELLAHDPVLGKTKLIAEAWDAG
                     GLYQVGTFPAYGRWAEWNGRYRDTLRRFLKGDAGQVQEMAQMVKGSPNMYLERGPTAS
                     INFVTCHDGFTLNDLVSYAEKHNLANGEQGRDGANDNYSWNWGHEGPTNDGEITRLRQ
                     RLMRNAVTLLMVSQGVPMLLMGDEIAHSKQGNNNTYAQDNELNWLNWHAVPDNADLLR
                     FVRHCVGFRRAHPALRRRYHLHERDLVDSGYPDISWHGTREFQPDWSHDSRTLAFMLC
                     GMHAALLPGRAADDYIYVAINMHWEAHTFELPRLRDRRRWYVFADTYQPSPNDITIPG
                     EERALRTQRNLFVGPRSVVILVGR"
     gene            2233..2658
                     /locus_tag="PK829_08650"
     CDS             2233..2658
                     /locus_tag="PK829_08650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU41323.1"
                     /translation="MNLEPLTIPGRADRLSQLLDYVSWAATAAGLNEASIYRLSLAVD
                     EIATNIVMHGYGAGGQPGDLTIWADRSPERLAIRLEDTGQPFDPREAPQPDNLDRPLE
                     ERQDGGLGIFLALWGVDDFTYERRGGVNRCTFVMRLEPG"
     gene            2663..4528
                     /locus_tag="PK829_08655"
     CDS             2663..4528
                     /locus_tag="PK829_08655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU41324.1"
                     /translation="MPVNYVQMLVVSDNDVASRRLTRSFAGWGWGVVRAGADADAALA
                     RLRAGGIDLVLLDLDAAEHDNYAFLSRWREEPGLSSVPVVITTQPGVALGRLARCVEQ
                     GASDYLSYPHQPVLLRARLQNVLQRKLLQEQAAMALNAFNEIEKIADDLRLVILPLGT
                     ALSAEADYDRLIARIVAEAQTISNADAGALYLADGEGRLHFRYVRVQSLDFTYSDPPG
                     GLVEFAPIPLSDPATGAPNDDSLPVHVVLSGESVNLTDIHAGRFDLSRVLGFEARFQP
                     YRLQSCLLVPLHSGQITGALLLANSRHPLSGEIGPFDAYHQLVAEALASLAAIVLNNR
                     LLSEREADLLRYKRELEIGREIQLSFLPARLPDPPGWELVSHFQPALEVAGDFYDAFR
                     LPHGHVALVIADVVGKGVTAALFMAIVRSLFRALFQQNYFQAEPAGSGAVIEAMTPFS
                     FVDRTALLNAIRLTNAYLITNHADAYAFATLWAAVLDPDSGRLLYVNAGHNPPLLLAR
                     GRDGRRTVREELEPTGPAIGLLPNAGFWLAETRLAADDILFAYTDGTTEARDAAGQQL
                     GEERLHALLSDARTAPEALAVVEEALREHTGDAEAFDDVTLLAVRRLPDAEETRD"
     gene            4574..>4901
                     /locus_tag="PK829_08660"
     CDS             4574..>4901
                     /locus_tag="PK829_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41325.1"
                     /translation="MLRGLSSVVCGLCAALALAGCGRAGGIAPVTSATVAATPTAEAT
                     TLPPTAMPTATPTQTVTPAAAASPMPVPTAVPSPDAASPDAPSPDAPSPTPDPYAELT
                     IDALAAR"
BASE COUNT          814 a         1706 c         1575 g          806 t
ORIGIN      
        1 caacatcgtc gtccgcaagc actttggcga catccgtgtg aagtcggagc cgggccagac
       61 gcagttcgag gtctggctgc cgcaggagtt ggaaggcgag taagcgccgg cagcgatgat
      121 caatcgtatt gacgtctacc caacccaccg ccacggcgaa ttctggttgc gcgccgggcg
      181 gccatatccc ttcggttcca ccatcgtgcc cggcggggtc aacttcgccg tcttctcgcg
      241 ccacgccacc gcttgcgccc tcgttctcta ccgccgcggc gaagccgagg cgatggtcga
      301 gattcccttt ctagaggcct tccgcatcgg caacgtctgg gcgatggtcg tctttgggct
      361 ggacattgag gagctggagt acggctaccg catggacggg ccgcaccggc cgcacgctgg
      421 ccaccgcttt gaccccagcc gcgtgctcct ggacccctac gcgcgggcca tcagcggccg
      481 tctgcgctgg gccgaagccc acgagccggc cgacgcgctg cccttccgcg ggcgtatcct
      541 gctggatgac ttcgattggc aggacgaccg ccaactggaa ctgccgtcgg aggacctgat
      601 catctacgag atgcacgtcc gcggcttcac gcgccaccca tcgtcgggcg ttaagtttcc
      661 aggcaccttc gccgccattc gcgagaagat cccctacctg gtcgaactgg gcatcaatgc
      721 cgtcgagcta atgcccgtcc aggagttcga cgaactggag aacgacttca cgaaccccta
      781 cactggccag aagctgcgca actactgggg ttactccacc gtcggcttct tcgcgcccaa
      841 agcgggctat gccgccactg gcgggctggg catggaggcc gacgagctaa aagcgctggt
      901 gaagctgctt caccgccacg gcatcgagat catcctcgac gtggttttca accacaccgc
      961 cgagggcaac gagaaagggc cgaccatttc cttccgcggg ctggacaacc gcgtctacta
     1021 tatgctgcaa ccggatggca gctaccagaa cttcagcggc acgggcaaca cgctcaactg
     1081 caacaacccc gttgtgcgca acctggtgct agacgcgctg cgctattggg tgtcggaata
     1141 ccacatcgac ggctttcgct tcgacctggc gtccatcctc ggccgcgatg ccgaaggcca
     1201 cccgttggcc aacccgccgc tgctggagct gttggcccac gatcccgtgc tgggcaagac
     1261 caagcttatc gccgaggcgt gggacgcggg cgggctatat caggtcggca cgttcccggc
     1321 ctatggccgc tgggcggagt ggaacggccg ctaccgtgac acgctgcgcc gctttctgaa
     1381 gggcgacgcc gggcaggtgc aggagatggc ccagatggtc aaaggttcgc ccaacatgta
     1441 cctggagcgc gggccgacgg cgtcgatcaa cttcgtcacc tgtcacgacg gcttcacgct
     1501 caacgacctg gtcagctacg ccgaaaagca caacctggcc aacggcgagc aggggcgcga
     1561 cggggccaac gacaactaca gttggaattg gggccacgag gggccaacca acgacggcga
     1621 gatcacgcgg ctgcggcagc ggctgatgcg caacgccgtg accttgctca tggtgagcca
     1681 gggcgtgccc atgctgctga tgggcgacga gatcgcccac agcaagcagg gcaacaacaa
     1741 cacctatgcc caggacaacg agttaaactg gctcaactgg cacgccgtgc cagacaacgc
     1801 cgatctgctt cgcttcgtgc gtcactgcgt cggctttcgc cgggcgcacc cggcgctgcg
     1861 ccgccgctat cacctccacg agcgcgacct cgttgatagc ggctacccgg acatctcctg
     1921 gcatggtaca cgcgagttcc agcccgactg gtcgcacgac agtcgcacgc tggccttcat
     1981 gctctgcggc atgcacgccg cgctgctgcc ggggcgggca gccgacgatt acatttacgt
     2041 ggccatcaac atgcactggg aggcccatac cttcgagctg ccgcgcctgc gcgaccggcg
     2101 acggtggtat gtctttgccg acacgtacca gccctccccc aacgacatca ccatcccggg
     2161 cgaggagcgc gccctacgca cacagcgcaa cctctttgtc ggcccgcgtt cggttgtcat
     2221 tctggtagga cgatgaatct tgaacccctg accatccccg gccgcgctga ccggctgtcg
     2281 cagttgctcg actacgtcag ttgggcagcc acggccgccg gactgaacga ggcgagcatc
     2341 tatcgcctct cgctggccgt tgacgaaatt gctaccaaca tcgtcatgca cggttacggg
     2401 gccgggggac agcccggcga cctgaccatc tgggccgacc gtagccccga acggctggcc
     2461 atccgtttgg aagatacggg ccagccgttc gacccgcgcg aagcgcccca gcccgacaac
     2521 ctcgaccggc cgttggagga gcggcaagat ggggggctgg gcatcttcct ggcgctctgg
     2581 ggggtggacg attttaccta tgagcggcgc ggcggggtca accgttgcac ctttgtgatg
     2641 cggctggagc caggctgagg tcatgcccgt caattacgtc cagatgttgg tcgtcagtga
     2701 taatgacgtg gccagtaggc ggctgacgcg ctcgtttgcc ggttggggct ggggggtcgt
     2761 gcgcgccggc gcggacgccg acgcggctct ggcgcgcctg agagccggtg gcatcgacct
     2821 tgttctcctc gacctggacg ccgccgaaca cgacaactac gcctttctga gccgctggcg
     2881 cgaggagccg gggctgagca gcgtgcccgt ggtcatcacc acgcagccgg gcgtggccct
     2941 gggccggctg gcccgctgcg tggagcaggg cgccagcgat tacctcagct acccccatca
     3001 gcccgttctg ctgcgggcgc gcctgcaaaa cgtcttgcag cgcaagctct tgcaggagca
     3061 ggcggccatg gccctgaacg ccttcaacga gatcgagaag atcgccgacg acctgcggct
     3121 ggtcattctg ccattgggca cggcgctctc ggccgaggcc gactatgacc ggctgatcgc
     3181 ccgcatcgtc gccgaggcgc agacgatcag caacgccgac gccggcgcgc tctacctggc
     3241 cgacggcgag ggccggctgc acttccgcta cgtccgcgtc caatcgctcg acttcaccta
     3301 cagcgatccg cccggcggcc tggtcgagtt cgcgcccatc cccctgtccg atccggcgac
     3361 cggcgcgccc aacgacgaca gtctgcccgt ccacgtcgtc ctgagcggtg agtcggtcaa
     3421 cctgaccgac atccacgccg gccgcttcga cctgagccgg gtgctgggct tcgaggcgcg
     3481 cttccagccc taccggctgc aatcgtgcct gctggtgccg ctacacagcg gccagatcac
     3541 cggcgcgctg ctgctggcca acagccgcca tccactcagc ggcgagatcg gcccgttcga
     3601 cgcctatcac caacttgtgg ccgaggcgtt ggcctctctg gcggccatcg tcctcaacaa
     3661 ccgcctgttg agcgagcgcg aggccgacct gctgcgctac aaacgcgagt tggaaatcgg
     3721 ccgcgagatt caacttagct tcctgcccgc ccggctgccc gacccgcccg gctgggagtt
     3781 ggtgtcccac ttccagcccg cgctggaggt cgccggcgat ttctacgacg ccttccggct
     3841 gcctcacggc cacgtggccc tggtgatcgc cgacgtggtt ggcaagggcg tgacggcggc
     3901 gctgttcatg gctatcgtac gcagcctttt ccgggccttg ttccagcaga actattttca
     3961 ggccgaaccg gccggcagtg gcgcggtgat cgaggcgatg acgccgttct ctttcgtcga
     4021 tcgcaccgcg ctgctgaacg ccatccggct gaccaacgcc tatctgatca ccaaccacgc
     4081 cgacgcctat gccttcgcca cgctttgggc ggccgtgcta gaccccgaca gcggccgtct
     4141 gctatacgtc aacgccggcc acaacccgcc gctgctgctg gcccgcggcc gcgatggccg
     4201 gcggacggtg cgcgaggaac tggagccaac tggcccggcc atcggcctgc tgcccaatgc
     4261 gggcttctgg ctggcggaga cacgcctggc ggccgacgac atcctctttg cctacaccga
     4321 cggcacgacc gaggcgcgcg acgcggccgg gcagcaactg ggcgaggagc ggctgcacgc
     4381 gctgctcagc gacgcgcgga cggccccaga ggcgctggcg gttgtggagg aggctttgcg
     4441 cgagcacacc ggcgacgcag aggcgtttga tgatgtgacg ctgctggccg tgcggcgctt
     4501 gccggatgca gaggagacaa gagattaacg caaggacgcc gggggcgcag agacgcaaag
     4561 gatgagagtg ggtgtgttgc gtgggctgtc gtctgtcgtc tgcggcctat gcgcggcgct
     4621 ggcgctggcg ggctgcggcc gggcgggcgg cattgcgccg gtgacgagcg cgaccgtggc
     4681 cgctacgcca acggctgagg cgacgacctt gccgccaacc gcgatgccga cagctacgcc
     4741 cacgcagaca gtcacgccgg cggccgccgc gtcgcccatg cctgtcccga cggccgtccc
     4801 gtcgcccgac gccgcgtcgc ccgacgcccc gtcgcccgac gccccgtcgc ccacgcccga
     4861 cccttacgcc gaactgacca tcgacgccct cgcggcgcgc a
//
LOCUS       DAOSMQ010000230         4881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17449_length_4881_cov_4.243058, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000230 DAOSMQ010000000
VERSION     DAOSMQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17449_length_4881_cov_4.243058"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..597)
                     /locus_tag="PK829_08665"
     CDS             complement(307..597)
                     /locus_tag="PK829_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOU41326.1"
                     /translation="MFAVVNHLHFDRPVEEFRAAVASEGMPLLAKQPGFRDFYLVKEA
                     EDRGIVIIFWEDAASAVAGAQAFGPTWFNEHIIPYLVSPQQRSAGEVLVSHH"
     gene            complement(640..1242)
                     /locus_tag="PK829_08670"
     CDS             complement(640..1242)
                     /locus_tag="PK829_08670"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU41327.1"
                     /translation="MKAGDVWFNPVTGMRMVVVQTPRETGGRSMTVEYELRPHTGRDA
                     APAHRHRIYTERFDILAGRAAYVCGDEERTAEAGETVVIPLDTTHVHPWSISDEALVV
                     RQTTEAARPDEATLTAALAGVRTGFDLAAEGKVDARGRPNALQAAVIFNALLPDNYLP
                     NIPLPAQRLLFGALAGLGRLLGYRLTYPRFEQQETAARMA"
     gene            complement(1330..1983)
                     /locus_tag="PK829_08675"
     CDS             complement(1330..1983)
                     /locus_tag="PK829_08675"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HOU41328.1"
                     /translation="MNRSFDRFGGLSAMMVGVLSILYAIFFLVITPRAEATGALGSWI
                     ILAISGVFSSAAFVALYLRLRPASEGFALWGLVLGLFSSFATLVHGAWQALLLTMLPA
                     ADQSQRAAIELVRMVVSPVDPAGVATFGVIGLASLVTGMLIIGSGRLPRMLGYLAVAN
                     AVLLVALYFATAAGAQTLILLSGGLTSVLVGPIYWVWLGRELRRETAPAAMAAPSMA"
     gene            complement(2101..2592)
                     /locus_tag="PK829_08680"
     CDS             complement(2101..2592)
                     /locus_tag="PK829_08680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOU41329.1"
                     /translation="MAEYKFVDHWQIHAPIEVVYRHVADPRTYARWWRDYDRVTIVKD
                     APFPHVGGRAEFLVRSPFGYRLFLDVTTVAAEAPRSITTETRGQLEGTGIWEFEETDG
                     VTHVTFTWIVRTHHPLINRLEWVAKPLFALSHAIVSGRGHRGLKRLLEKEQSGQWSVA
                     SDQ"
     gene            complement(2691..3317)
                     /locus_tag="PK829_08685"
     CDS             complement(2691..3317)
                     /locus_tag="PK829_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41330.1"
                     /translation="MIDQFMTATFDTTQTGDVSLEAAETYLQAAQRCLAGDRPAGLEA
                     LNDLFHSGSVARPDGRTRGQLVALDIAPGLTPLLGGLLARWRPWQGKVFDRDANRGEN
                     VFTRDSYLPARAIWPFYRRYSDDGPATYRAFPFRTSAGPARDDPGRAVLRLDYDLPAN
                     PRAAISVRRVLDELVQVADGLYLGKAYVHWYRSNWSLVAYFALRPVLI"
     gene            complement(3314..3982)
                     /locus_tag="PK829_08690"
     CDS             complement(3314..3982)
                     /locus_tag="PK829_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41331.1"
                     /translation="MNTPMETASNAVHTFDPDRVAALETRSWRQYYDRDWLGMFRTLV
                     ALHREQFSLSHLGALLATLSASRAARTFAPLESSDPDRARQQLVPYYRRARVALGSAA
                     SAETLAAREVDYWIVHRRLAIQRKANPAAADPDALDDIQPMVDAFARLHAALFNSTPA
                     DTRPSAEYRALAAKAVDRITGGYSDDIPADWARTEDYLRRAYRAVAAVVEARPIRPQA
                     EVTP"
     gene            complement(3979..>4881)
                     /locus_tag="PK829_08695"
     CDS             complement(3979..>4881)
                     /locus_tag="PK829_08695"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOU41332.1"
                     /translation="SRNRFLPTALSIVSHNVALIAGIIASRFIPGLGILGPTFGVLGG
                     ALLQVIVLSPGLRAGEGRLSLAFNPADSRLREVVRLLIPNGLSVGVNYSGFIVDTSFA
                     TRAPQVAGLAALYNAFLLVGLPIALLGQAVGQAAFPRLTAHAEAGDWRQLRRTQAASL
                     AAAVGLALGAVAGLLLLGRLVIRILFERGAFDAAAGDLTYRVLVIYAIALPAYIATEV
                     ITRGLIALRDTRTPLLTNTGQLVGRVAVIVALLPAFGVLAIPAAFAITSTLETLTLGA
                     VLWRKLARAMPDQPAGPLSGEMMP"
BASE COUNT          828 a         1597 c         1706 g          750 t
ORIGIN      
        1 tctcttgacc gctgaggagc ttaaccaaaa tgcctgttaa tttgttaatg tcctatcagg
       61 ctgtcgttgc tggtcacagc ctaacaggct gtgctacagg ctgagcgacg ggcttactgt
      121 agaacagcct gtcaggctgt cgtcgctggt cacagcctga caggctgttc tacaggctgt
      181 gctacaggct ggttgtagca cagcctgtca ggctgtcgtc gctggtcaca gcctaacagg
      241 ctgtgctaca ggctgtgcta cgggctgtgc gactactgaa aagccctgtc acgcctgcgg
      301 ataggattag tggtggctga ccaacacctc gcccgcgctg cgctgttgcg ggctgaccag
      361 ataggggatg atatgctcgt tgaaccaggt tgggccaaag gcctgcgctc cggccacggc
      421 actggcggcg tcttcccaaa agatgatgac gatgccgcga tcctcagcct ctttgacgag
      481 gtagaagtct cggaagccgg gctgtttggc cagcaggggc atgccctcgc tggctacggc
      541 ggcgcgaaac tcctccaccg gccggtcgaa atgaagatga ttgacgactg cgaacatgct
      601 ctctgtctcc tgaaaagcct actgatttcg gtccatcgct catgccattc gcgcggcggt
      661 ctcttgctgc tcgaagcggg ggtaggtgag ccggtagccc agcagccgcc ccagcccggc
      721 cagcgcgcca aaaagcagcc gctgcgcggg cagggggatg ttgggcaggt agttgtcggg
      781 cagcagggcg ttgaagatga ccgccgcttg tagcgcgttc ggccggcccc gcgcgtccac
      841 tttgccctcg gcggccaggt cgaagccggt gcgcacgccc gccagcgccg ccgtcagcgt
      901 ggcctcgtcc ggccgggcgg cctccgtcgt ctggcgcacg accagcgcct cgtcgctgat
      961 gctccagggg tggacgtggg tcgtgtccag ggggatgacg accgtctcac ccgcttcggc
     1021 cgtgcgctcc tcgtcgccgc aaacgtaggc ggcccgcccg gccagaatgt cgaagcgctc
     1081 ggtatagatg cggtggcggt gggccggagc agcgtcccgg ccagtgtggg gccgcaactc
     1141 atactccaca gtcatcgacc ggccgcccgt ctctcgcggc gtctgcacga cgaccatccg
     1201 catccccgtc accggattga accacacatc ccctgctttc atggccttgc tcctgttgtc
     1261 gtcccatgga cgacagaccc cggcgaagct cctctggccg ccgtccgcgg tctgtcgtcc
     1321 gccgtcgttc taagccatcg acggcgcggc catcgccgcc ggcgcggtct cgcggcgcag
     1381 ttcgcggccc agccagaccc agtagatcgg cccgacaagc accgaggtca gcccgcccga
     1441 cagcaggatg agcgtctgcg ccccggcggc ggtggcgaag tagagcgcca ccagcaggac
     1501 ggcgttggcc acagccagat agcccagcat gcgcggcaac cgtccgctgc cgatgatgag
     1561 catgcccgtc accagcgaag ccaggccgat gacgccgaag gtggccacgc cggccgggtc
     1621 cactggcgag acgaccatgc gcaccagttc gatagccgcg cgttgcgact ggtcggccgc
     1681 cggcagcatc gtcagcagca acgcctgcca ggccccatgc accagcgtgg cgaagctgct
     1741 gaacagcccc agcaccagcc cccacagcgc gaagccctcc gacgccggcc gcaaccgcaa
     1801 gtagagggcc acgaaggccg cgctggagaa gacgccgctg atggccagga tgatccagct
     1861 gcccagcgcg ccggtggctt cggcgcgcgg cgtgatgacc agaaagaaaa tcgcgtacag
     1921 gatcgacaac acgccgacca tcatcgcgct caatccgcca aaacgatcga aggaacggtt
     1981 cattttgctc tccttgaact atctaaagtc gcacgaatca cgatgaaaac gatgaccaac
     2041 agccgaccgc cgaccgccga ccgcattact ggccactggc tactgtccac tgaccactgg
     2101 ttactggtca ctggccaccg accactgacc actctgctct ttctctagca accgcttcaa
     2161 cccccgatgc ccgcgcccgg acacaatggc atggctgagg gcgaacagcg gcttggcgac
     2221 ccactccagg cggttgatga gcgggtgatg ggtgcggacg atccaggtga aggtcacgtg
     2281 ggtcacgccg tccgtctcct cgaattccca gatgcctgtg ccctccagtt ggccgcgcgt
     2341 ctcggtggtg atggagcgcg gcgcttcggc ggcgacggtg gtcacgtcga gaaatagccg
     2401 gtagccgaag ggagaacgca ccaggaactc cgcccggccg ccgacgtggg ggaagggcgc
     2461 gtccttgacg atggtcacgc ggtcgtagtc gcgccaccaa cgcgcgtagg tgcgtgggtc
     2521 ggccacgtgc cggtagacca cctcaatcgg ggcgtgaatc tgccagtgat cgacaaactt
     2581 gtattcggcc atctgtcgct tacctctctc ttgcggcgat gccattagga tacgcgccgg
     2641 cgcggccatc cgacagcccg ccggatgctc attcgggggc caatcgctgc tcaaatcaag
     2701 accggccgca gggcgaagta ggccaccagc gaccagttgc tccggtacca gtgcacgtag
     2761 gccttgccca ggtagaggcc atcggccacc tgcaccaact cgtcgagcac gcggcggacg
     2821 ctgatggcgg cgcgggggtt ggcgggcagg tcgtagtcga gccgcagcac ggcgcggccg
     2881 gggtcgtcac gggcggggcc agcgctggtg cggaacggga aggcgcggta ggtcgccggg
     2941 ccgtcgtcac tgtagcggcg gtagaagggc cagatcgccc gtgcgggcag gtaagagtcg
     3001 cgggtgaaga cgttctcacc gcggttggcg tcgcggtcaa agaccttgcc ctgccacggc
     3061 cgccagcggg ccagcagccc acccagcagc ggcgtcagcc ccggagcgat gtcgagggcg
     3121 acgagctggc cacgggtgcg accatcgggc cgggccacgc tgccggagtg aaagagatcg
     3181 ttgagcgcct ccagccccgc cggccggtcg ccggccaggc agcgttgggc cgcttgcaag
     3241 taggtctcgg ccgcctccag cgagacatcg ccggtctgcg ttgtgtcgaa cgtcgcggtc
     3301 atgaactggt cgatcatggc gtcacctctg cttgcggccg tatgggccgt gcctccacga
     3361 cggcggcgac agcgcggtag gcgcgccgca ggtagtcctc cgtgcgcgcc cagtcggcgg
     3421 ggatgtcgtc ggagtagccg ccggtgatgc ggtcgacggc cttggccgcc agggcgcggt
     3481 actcggccga ggggcgcgta tccgccggcg tgctgttgaa cagcgccgcg tgcagccggg
     3541 cgaaagcgtc caccatcggc tggatgtcgt cgagcgcgtc gggatcggcg gcggccgggt
     3601 tggctttgcg ctggatggcc aatcggcgat ggacgatcca gtaatcgacc tcgcgggcgg
     3661 ccagcgtctc ggcgctggcg gcgctgccca gggccacccg cgcccggcga tagtagggca
     3721 cgagctgctg tcgcgcccgg tcggggtcac tgctttccag tggcgcgaag gtgcgcgccg
     3781 cccggctggc gctcagcgtg gccaacagcg cccccaggtg agacaggctg aactgctcgc
     3841 ggtggagggc gaccagcgtg cggaacatcc ccagccaatc gcggtcgtaa tattgccgcc
     3901 acgacctggt ttccagggcg gcaacgcggt cggggtcgaa ggtgtggacg gcgttcgacg
     3961 cggtttccat gggcgtgttc atggcatcat ctccccggat agtgggccgg caggctggtc
     4021 gggcatcgcc cgggcgagct tgcgccagag cacggccccc agtgtcaatg tctccagcgt
     4081 gctggtgatg gcgaaggccg ccggaatggc caggacgccg aatgcgggca gcagcgcgac
     4141 gatgaccgcg acgcggccga cgagttgccc ggtgttggtc agcagcggcg tgcgcgtgtc
     4201 gcgcagggcg atcaggccgc gggtgatgac ctcagtggcg atgtaggccg gcagggcgat
     4261 ggcatagatg accaggacgc gataggtcag gtcgcccgcg gcggcgtcga acgcgccgcg
     4321 ctcgaagaga atgcgaataa ccagccggcc gagcagcaac agaccggcga ccgcccccag
     4381 cgccaggcca acagccgcgg ccagcgacgc cgcctgcgtg cggcgcaatt gccgccagtc
     4441 gcccgcctcg gcgtgggctg tcaggcgcgg gaaggcggcc tggccgaccg cctggcccag
     4501 cagggcgatg ggcaggccga cgagcaggaa ggcgttgtag agcgccgcca gcccggccac
     4561 ctgtggcgcg cgcgtggcga aagaggtatc gacgatgaag ccggagtagt tgacgccgac
     4621 agacaggccg ttggggatga gcaggcgcac gacctcgcgc aggcggctat cggccgggtt
     4681 aaaggccaac gatagtcgcc cctcgcccgc gcgcaggccg ggcgagagga cgatgacttg
     4741 cagcagcgcc ccgccgagga cgccgaatgt ggggccgaga atgcccagcc cggggatgaa
     4801 gcggctggcg atgatgccgg caatgagggc gacgttgtgg ctgacgatgg acagggccgt
     4861 gggcaggaag cggttgcggc t
//
LOCUS       DAOSMQ010000231         4878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17464_length_4878_cov_5.362223, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000231 DAOSMQ010000000
VERSION     DAOSMQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17464_length_4878_cov_5.362223"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PK829_08700"
     CDS             complement(<1..1055)
                     /locus_tag="PK829_08700"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU41333.1"
                     /translation="MTDDKMNEATGGVALPGKALALPHGSLPLPAFLPDGTQGVVRAV
                     SADDLEAAGVRAVQMNVYHLMQRPGTSTIQVLGGLHRLAGWSGPIFTDSGGFQVYSLI
                     RQNPKSGSITDRGAVFRPGGDRKYQLTPEKSIQLQFGYGSDMLICLDDCTGPDDPPAT
                     QAESVRRTVRWAAACKRTYLRLVEQKRLGEAERPRLMAVVQGGLSRELRRACAEALLA
                     IGFDAYGYGGWPLDSEGRLLIDMLATVRELVPAQFPVHALGVGHPPSVAACVGLGYEL
                     FDSTMPTRDARHGRLYVFRRDPAAAPLAGADWFGYVYPGDDKHVKAAGPPSSFCDCPT
                     CRRYSLGYLHHLFKISDT"
     gene            1075..>2231
                     /locus_tag="PK829_08705"
     CDS             1075..>2231
                     /locus_tag="PK829_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU41334.1"
                     /translation="MKRQPQPQTAPPPEFLAKMRALLPEGEFDAFLAAYDRPPIAGLR
                     VNTLKIAPPDFTSLAPFSLAPVGAWEPAAFTVTGDSRPGRHPYHDAGLYYLQDPSAMT
                     AAVALSPRPGELVLDLAAAPGGKATHLAARLAGGAGGGSPLRRALDDDGLLVANDVHA
                     GRARLLADNLARWGAINTVVTQDEPERLAAAFGPVFDRVLIDAPCSGEAMFRRLGEIE
                     WSAGIVAACARRQRGVLAVAPGLVRPGGRLLYATCAFSPEENEAVVAAFLAENRDFRL
                     IAPPAIAAADHGRPDWAGAAADPAVAAGLANALRLWPHHFPGEGHFLALMARDDAAEA
                     GEPPPFARRPPSPAQLRLWRDFAAATLTLDLPAERLHVHGERLYLLPRRAVA"
     assembly_gap    2232..2331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2346..3659)
                     /locus_tag="PK829_08710"
     CDS             complement(2346..3659)
                     /locus_tag="PK829_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOU41335.1"
                     /translation="MEHKQQLLKRIADRQARIGIVGLGYVGLPLAVAFAEEGFTVVGV
                     DLDARKVAALQRGESYVEDVPASVITTLRDRGLFRASSDYATLADVDAISICVPTPLR
                     KTKDPDISYIVAATECIATYMPAAGGQLIVLESTTYPGTTEEIVLPRLSRDGLQVGRD
                     FFLAFSPERIDPGRTDYTVRTTPKVIGGATPDCLEVAVALYGTVVDRPVPVSGTAAAE
                     MVKLLENTFRAVNIGLVNEVALMCDRLGLNVWEVVGAAATKPYGFMPFYPGPGLGGHC
                     IPIDPHYLSWKLRTLNYTARFIELAAEVNSHMPDYVVGKVGDALNAERKAINGSRVLV
                     LGVAYKRDVGDVRESPALDVIHLLQQRGAEVSYHDPYVGSLRHEGLDMDSSPLTDAAL
                     AAADCVVVVTDHSAFDWDHIGATARLIVDTRNALKGRAAQRVVTL"
     gene            complement(3857..>4878)
                     /locus_tag="PK829_08715"
     CDS             complement(3857..>4878)
                     /locus_tag="PK829_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41336.1"
                     /translation="FRPVGSGFVPLVLAALAPLAAALMAAPAGVALVLLAVGLGVLAA
                     ALYGGRYEAAAAAWRAFLMGVLGLLPLLWVAWAGAAGQATGEPVRLGLLLATLLLLGG
                     FPFHIWLRGLAHWASAGALALALGLGQVVVVALLVGLLDVVPAVRAAPEFQTALRGSA
                     VMAALLAVFLLAHEQTARGALGAALTLDAGWLTLTALTPGAAGLTIALAALAGRTLSL
                     LLIALGWRWPASGRAARLGPVLRGYGFASLLGLPLTPGFAARWAQASLLGGGAWAALG
                     IAALAVAAWALWWARPAGSPPVEAEAIAPGRDEAIAATVLLGVAALLGLFPGLLTALL
                     ARLAG"
BASE COUNT          693 a         1768 c         1689 g          628 t
ORIGIN      
        1 gtgtcgctga tcttgaagag atggtgcagg tagccgaggc tatagcggcg gcaggtgggg
       61 cagtcgcaga acgacgaggg cggcccggcc gccttgacgt gcttgtcgtc gccggggtag
      121 acgtagccga accagtccgc gccggcgagc ggggccgcgg ccgggtcgcg ccggaagacg
      181 tacagccggc cgtggcgggc gtcgcgggtg ggcatggtgc tgtcgaacaa ctcgtagccc
      241 agccccacgc aggcggcgac gctcggcggg tggcccacac cgagggcgtg caccgggaac
      301 tgggccggga ctagctcgcg cacggtggcc agcatatcga tcagcagccg cccctcgcta
      361 tcgagcggcc agccgccgta gccgtaggcg tcaaagccga tggccagcag cgcctcggcg
      421 cacgcgcgcc gcaactcgcg cgacagcccg ccctgcacca cggccatcag tcgcgggcgc
      481 tccgcctcgc ccaggcgctt ctgctccacc agccgcaggt acgtccgctt gcacgccgcc
      541 gcccagcgca ccgtccgccg caccgattcg gcctgcgtcg ccggtgggtc gtccgggccg
      601 gtgcagtcgt cgaggcagat gagcatgtcc gacccgtagc cgaactggag ttggatgctc
      661 ttctccggcg tcagctgata tttgcggtcg ccgcccggcc ggaagacggc cccgcggtcg
      721 gtgatgctgc cgctcttggg gttctgccgg atgagcgagt atacttggaa gccgccggag
      781 tcggtgaaga tggggcctga ccagccggcc agccggtgca gcccgccgag gacctggatg
      841 gtcgaagtgc ccggccgctg catcaggtgg tagacgttca tctgcacggc gcgcacgccg
      901 gcggcctcca gatcgtcggc cgagacggcg cgcacgacgc cctgcgtccc gtcgggcaga
      961 aaggccggca gcggcagcga cccgtggggc agggccaacg ccttgccggg caaagcgacg
     1021 ccgccggttg cctcgttcat tttgtcatcc gtcatacacg tactcatcca ccacatgaag
     1081 cgacagccgc agccacagac cgcgcccccg ccggagttcc tggccaagat gcgcgccctg
     1141 ctacccgagg gcgaattcga cgcctttctg gccgcctacg accggccgcc aatcgccggg
     1201 ctgcgcgtca acacgctcaa gatcgcgccc ccggatttta ccagcctcgc gccgttctcg
     1261 cttgcgccgg tcggggcgtg ggagccggcc gccttcaccg tgaccggcga cagccgcccc
     1321 ggccgccacc cctaccacga tgccggcctc tactacctgc aagacccgtc ggccatgacc
     1381 gcggccgtgg ccctctcgcc ccggccgggc gagcttgtcc tcgacctggc cgccgcgccg
     1441 ggcggcaagg ccacccatct ggcggcgcgc ctggccggcg gggcgggcgg cggctcgccc
     1501 ttgcgccgcg ctctggacga cgatggtctg ctggtggcca acgacgtcca cgccggccgc
     1561 gcccggctgc tggccgacaa tctggcccgc tggggcgcga tcaacaccgt ggtgacgcag
     1621 gacgagccgg agcggctggc ggccgccttc gggccggtgt tcgacagggt gctgatcgac
     1681 gcgccctgct ccggcgaggc catgttccgc cgtctggggg agatcgagtg gagcgccggc
     1741 attgtcgccg cctgcgcccg gcggcagcgg ggcgtgctgg ccgtggctcc cggcctggtg
     1801 cggcccggcg ggcggctgct ctatgccacc tgcgccttct cgccggagga gaacgaggcc
     1861 gtcgtggccg ccttcctggc cgagaaccgc gacttccggc tgatcgcccc gccggccatc
     1921 gccgcggccg accacggccg gccggattgg gccggcgcgg cggctgaccc agccgtggct
     1981 gccggcctgg ccaatgccct ccgcctctgg ccccaccact tccccggcga gggccacttc
     2041 ctggccctga tggcccgcga cgacgcggcc gaggccggcg aaccgccgcc tttcgcccgc
     2101 cgcccgccgt cgccggcgca actgcgcctg tggcgcgact tcgccgcggc gaccctgacg
     2161 ctcgacctgc cggccgagcg cctgcacgtc cacggcgagc gcctctacct gttgccgcgc
     2221 cgcgccgtgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcgccctg
     2341 cgccgctaga gcgtcaccac gcgctgcgcg gcccgcccct tcagcgcgtt gcgcgtatcg
     2401 acgatgagcc gcgccgtcgc gccgatgtgg tcccagtcga aagcgctgtg gtcggtgacg
     2461 acgacgacgc aatcggccgc ggccagcgcc gcgtcggtca gcggcgacga gtccatatcc
     2521 agaccctcgt gccgcaacga gccaacgtag ggatcgtggt agctgacctc tgcgccgcgc
     2581 tgctgcaaca gatggatcac atctagcgcc gggctttcgc gcacgtcgcc gacatcgcgc
     2641 ttataggcca cgcccagcac cagcacgcgg ctgccgttga tagccttgcg ctcggcgttg
     2701 agggcgtcgc ccaccttgcc gacaacgtag tcgggcatgt ggctattgac ctcggcggcc
     2761 agctcgatga agcgggcagt gtagttgagc gtgcgcagct tccagctcag gtagtggggg
     2821 tcaatgggga tgcagtggcc gcccagaccc ggccccgggt agaagggcat gaagccatag
     2881 ggcttcgtcg ccgccgcgcc aaccacttcc cagacgttca ggcccagccg gtcgcacatc
     2941 agggccactt cgttgaccag accaatgttg acggcgcgga aggtgttctc cagcagcttg
     3001 accatctcgg ccgcggctgt gccggagacg ggcacgggcc ggtcgacgac cgtgccgtag
     3061 agggccacgg ccacctccag gcagtcgggc gtcgccccgc cgatgacctt gggcgttgtc
     3121 cgcacggtgt aatcggtgcg gccggggtcg atgcgctccg gcgagaaggc caggaagaag
     3181 tcacggccga cctgtaggcc gtcgcggctg aggcgcggca ggacgatctc ttcggtcgtg
     3241 cccggatagg tcgtgctctc caggacgatg agctggccgc cggcggcggg catgtaggtg
     3301 gcgatgcact cggtggcagc gacgatgtag gagatatcgg ggtccttcgt cttgcgcagc
     3361 ggcgtgggga cgcaaatcga gatcgcgtcc acgtcggcca gggtggcgta gtcgctgctg
     3421 gcgcggaaca ggccgcggtc gcgcagcgtg gtgatgacgc tggcgggcac gtcctcgacg
     3481 tagctctcgc cccgttgcag cgcggccacc ttgcgcgcgt ccaggtcaac gccgacgacg
     3541 gtgaagcctt cctcggcgaa ggccacggcc agcggcaggc ccacgtagcc aaggccgacg
     3601 atgccgatgc gcgcctgccg gtcggcaatg cgcttgagta gttgttgctt gtgctccatg
     3661 ctcgttcctt accccggcac tgggggtttc gatgcagttt gcggcgcggg ccgcgcgagc
     3721 gcccaagttc agctgcgcgg gacgacgagc gccggttggt gactggccta atcatacctt
     3781 aagaggggcc ggttccgata cgggcgcggg gcagatttca cgcctttttc gaccggccgc
     3841 cgggctgggg cgcggtctag ccggcgaggc gggccagcag cgccgtcagc agaccgggga
     3901 agaggcccag cagcgccgcc acgcccagca acacggtcgc ggcgatcgct tcgtctcggc
     3961 cgggagcaat ggcttcggcc tcaaccggcg ggctacccgc cggccgcgcc caccacagcg
     4021 cccaggcagc cacggccagc gcggcgatgc ccagcgccgc ccatgcgccc ccgcccaaca
     4081 gcgacgcctg cgcccaccgg gctgcaaagc ccggtgtcag cggcaggccg agcagcgacg
     4141 cgaagccata gccccgcagt actggcccca ggcgcgccgc gcgaccgctc gccggccaac
     4201 gccagccgag ggcgatgagc agcagactca gcgttcgccc ggccagcgcc gccagagcga
     4261 tggtcaggcc ggccgcgccc ggcgtcagcg ccgtcagggt cagccagcca gcatcgagcg
     4321 tcagggccgc gcccagtgcc ccgcgcgccg tctgctcgtg ggccagcagg aagacggcca
     4381 gcagggcggc catgacggcg ctgccgcgca acgccgtctg gaactctggc gcggcgcgca
     4441 cggcgggcac aacatccagc agcccgacca gcagggccac gacgacgacc tgccccaggc
     4501 ccagggccaa cgccagcgcc cccgccgatg cccaatgggc cagcccgcgc agccagatgt
     4561 ggaacgggaa gccacccagc aggagcagcg tcgccaggag caggcccagc cgcaccggtt
     4621 cgcccgtcgc ctgtccggcc gcgccggccc aggcgaccca gaggagtggc agcagtccca
     4681 ggacgcccat taggaaagcg cgccaggcgg ccgcggcggc ttcgtagcgc ccgccgtaga
     4741 gggcggcggc cagcacgccc agccccaccg ccagcaacac cagcgccacg cccgccggcg
     4801 cggccatgag cgccgccgcc agcggcgcga gggccgccag caccaggggg acaaacccac
     4861 tgcccaccgg ccggaacc
//
LOCUS       DAOSMQ010000232         4829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17649_length_4829_cov_4.138458, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000232 DAOSMQ010000000
VERSION     DAOSMQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17649_length_4829_cov_4.138458"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="PK829_08720"
     CDS             complement(<1..1711)
                     /locus_tag="PK829_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU41337.1"
                     /translation="MDAQTLPQHFLRQAQTQPASKVALRQKEFGIWREFSWADSYAEM
                     RALGMGLIVLGARRGDTIATVGDNDRYHLWSYLALQAIGAAQVGLFTDATPAEMAYII
                     DHSDATFVLAKDQEQCDKLLDIRAQIPKVHRVIYWDERGLWHYDEPWLLPFAEVQALG
                     RELIAREPDRFETEIALGAASDPAMLCYTSGTTGRPKGVVLSHGNILSAIRLYHDVDP
                     RRDTDNHVSFMPMGWIAEPILGIAAHVFAGLILNFPEEPETVRQNIREIAPEMLFYNS
                     RLWDSLVGTVQVRMNDASWLNRTLYRRFLPIGYRMADARFGGQPAGPAARAAYALGDR
                     LVFGPLRDQLGLSRVRAAYTAGSALSPDAMRFFHALGVNLKQIYGSTEVTGGATIHRD
                     AAIKFASVGPPAPGIDIRIAGDGEIQIAGPTVMQGYYKDPETTARDILTDADGRRWFR
                     TGDAGTIDDDGHLIYLDRVKDMLTLAGGERFSPQFVEGRLKFSPYVRDVMAIGGETRE
                     FATALIIIDYENVGQWAEKQRIPYTTFVDLSQRPPVYDLIRRAVEEVNASLPPGARLR
                     RFVL"
     gene            complement(1786..2562)
                     /locus_tag="PK829_08725"
     CDS             complement(1786..2562)
                     /locus_tag="PK829_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41338.1"
                     /translation="MDRSAAILSVEEVSLHFGGNTALSDVGFDVRPGEIRAIIGPNGA
                     GKTSILNCISGFYRPQQGRILFKGQTDLTRTPTDAIAALGLARTFQNIALYTGLSTLD
                     NLMAARHIHMKQSALQSMLYWGAAQREEVRHRQVVEEIIDFLEIAHIRKAVVGALPYG
                     LRKRVELGRALAMEPDLLLLDEPMAGMNLEEKEDMARFILDTYERRGVTIVLIEHDMG
                     LVMDISDRVVVLDFGRVIADGPPDAIKQDEKVIQAYLGEA"
     gene            2741..3154
                     /locus_tag="PK829_08730"
     CDS             2741..3154
                     /locus_tag="PK829_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOU41339.1"
                     /translation="MTAVIPDSHLSLLTGPVYAVLTTIAPDGQPENSIVWCSWDGTHV
                     VVNSTDSRRKTKNATRNPLVALTAVDPKDPFHWIDVRGYVEEIVPDPEYKTIDAHAKL
                     YCGVEHYYGDYAPIERRGTENRVDLKIRPTRVVAY"
     gene            3158..3760
                     /locus_tag="PK829_08735"
     CDS             3158..3760
                     /locus_tag="PK829_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41340.1"
                     /translation="MPNGALPDKPPGELARAIIGAEYAYVAQTAFQANEDRARAWQYF
                     FVTFATLIAALLSTQIESIDRRQLYLTFVVIFALLAGLGLITILQLVRLRQAWLESVQ
                     AMNQIKARLMADDPALAAYFRWPAATVPPAFKLRSFGFLQAVSVALLSGLAVGAAIAF
                     AALAGGQAVVPWAVSLLIGGASAAGILWLGYVRPLQRMKT"
     gene            3848..>4829
                     /locus_tag="PK829_08740"
     CDS             3848..>4829
                     /locus_tag="PK829_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOU41341.1"
                     /translation="MRRLIAAFVSALLFAVGCTSQPSADSVATAIVLTQQAQPTATPL
                     PEPTATPTPLPTATATPAPLTPTGIFESLSPAVVRIEADSGFGSGVLIEGNYILTNAH
                     VVWPAQDAIVTFPDGTTLAAVPVVGVDLLADLAVLGPVETDVDPLALSDNQELAIGDT
                     LLLIGYPGDVTSAPKPTLTETLISRLREQETFDITYFQVSAPVAGGQSGGIAVTMDGE
                     VVGLTGNRITEAQYGLIAAMADIRSRVDSIVAGQGEPASGRGPDLLEAADRHFVRLHR
                     TDEMRIFTADLDKGTELEVTVDGNGDALMQLLGPTPDDSALANETSHGIET"
BASE COUNT          791 a         1566 c         1623 g          849 t
ORIGIN      
        1 tcagcacaaa gcgccgcagc cgcgcccccg gcggcagcga ggcgttgacc tcctcgacgg
       61 cgcggcggat gaggtcgtag accggcggcc gctgcgacaa atccacgaac gtcgtgtacg
      121 ggatgcgctg cttctcggcc cactggccga cgttctcgta gtcaatgatg atcagcgccg
      181 tggcgaattc gcgcgtctcg ccgccgatgg ccatcacgtc gcgcacgtag gggctgaact
      241 tcagccgccc ctcgacgaac tgcggcgaga agcgctcgcc cccggccagc gtcagcatgt
      301 ccttgacgcg gtcgaggtaa atcaggtggc cgtcgtcgtc gatggtgccg gcgtcgccgg
      361 tgcgaaacca gcgccgaccg tcggcgtcgg tgaggatgtc gcgggcggtg gtctcagggt
      421 ctttgtagta gccctgcatc accgtcggcc cggcgatctg gatttcgcca tcgccggcga
      481 tgcggatgtc gatgcccggc gcgggcgggc cgacgctggc gaacttgatg gccgcgtcgc
      541 ggtggatggt cgccccgcca gtcacttcgg tcgagccgta gatctgcttc aggttgacgc
      601 ccagggcgtg gaagaagcgc atcgcgtcgg ggctgagggc cgagccggca gtgtaggcgg
      661 cgcggacgcg cgacaggccc agctgatctc gcagggggcc gaagacgagc cggtcgccca
      721 gggcataggc ggcgcgggcg gccggcccgg ccggctggcc gccaaagcgg gcgtcggcca
      781 tgcggtagcc gatgggcagg aaacggcggt agagcgtccg gttgagccag ctggcgtcgt
      841 tcatgcgcac ctgcaccgtg cccaccagcg agtcccacag gcgtgagttg tagaagagca
      901 tctccggagc gatctcgcgg atgttctgtc gcaccgtctc cggctcttcg gggaagttga
      961 ggatcagccc ggcgaagacg tgggcggcga tgcccaggat cggctcggcg atccagccca
     1021 tgggcatgaa gctgacgtgg ttgtcggtgt cgcggcgggg gtcaacgtcg tggtagaggc
     1081 ggatggcgct gaggatgttg ccgtggctga ggacgacgcc tttcggccgg ccggttgtgc
     1141 cggaggtgta gcatagcatg gccgggtcgc tggccgcgcc cagggcgatc tcggtctcga
     1201 agcggtcggg ttcgcgggcg atcagctccc gtcccaacgc ctgaacttct gcgaagggca
     1261 gcagccacgg ctcatcgtag tgccacaggc cgcgctcgtc ccagtagatg acgcggtgga
     1321 ccttgggaat ctgggcgcgg atgtcgagca acttgtcgca ctgctcctgg tctttggcca
     1381 ggacgaaggt ggcgtcgctg tggtcgatga tgtaggccat ctcggccggc gtggcgtcgg
     1441 tgaacaggcc aacctgggcc gcgccgatgg cttgcagggc caggtagctc cacaggtggt
     1501 agcggtcgtt gtcgccgacg gtggcgatgg tgtcaccgcg tcgcgctccc agcacgatca
     1561 gccccattcc caacgcccgc atctccgcgt aggagtcagc ccagctgaat tcgcgccaga
     1621 tgccgaactc cttttgccgc agggcgacct tgctggccgg ctgcgtctgt gcctggcgga
     1681 ggaagtgttg ggggagggtc tgtgcgtcca taattaggaa ttaggaattg ggaacgaaga
     1741 gagcgtcaca aacgcggcgc tcttcattcc taattcctaa ttgattcatg cctctcctaa
     1801 ataagcctga atcaccttct cgtcttgctt gatggcgtcg ggcgggccat cggcgatgac
     1861 ccggccgaag tcgagcacca cgacgcggtc ggagatgtcc atcaccaggc ccatgtcgtg
     1921 ctcaatgagg acgatggtga cgccgcgccg ctcgtaggta tcgaggatga agcgggccat
     1981 gtcctctttc tcctccaggt tcatacccgc cattggctcg tccagcagca gcaggtcggg
     2041 ttccatggcc agagcgcggc cgagttccac ccgcttgcgc aggccatagg gcagcgcgcc
     2101 gacgacggcc ttgcgaatgt gggcgatttc caggaagtcg atgatctcct ccaccacctg
     2161 acgatggcgc acttcttcgc gttgggccgc gccccagtag agcatcgact ggagggcgct
     2221 ctgcttcatg tggatgtggc gcgcggccat caggttgtcg agcgtgctca ggccggtgta
     2281 gagggcgatg ttctggaagg tgcgcgccag gccgagcgcg gcgatggcgt cggtgggtgt
     2341 gcgggtcagg tcggtctggc ccttgaagag gatgcgcccc tgctgcgggc ggtagaagcc
     2401 gctgatgcaa ttgaggatgg aggtcttgcc cgcgccgttg gggccgatga tggcccgtat
     2461 ttcgccggga cgcacgtcga agcccacatc gctcagggcg gtgttgcccc cgaagtggag
     2521 cgatacttct tcaactgaga ggatcgcggc gcttctatcc atgtagtctg gtaacggaac
     2581 gagcagctaa ccgcgcggga aaatatcata gttgtctctg gccggcaaca agttgaccaa
     2641 gcaatcatcg gcccggattg ttacaactgt ctggccggtg gccgcgtttc tgctacagta
     2701 cggtgagcca acatcacccc tagcatagga ggccacagcc atgaccgctg ttatcccgga
     2761 ctcacatctg tctctgctca ccggcccggt ctatgccgtg ctgacgacca tcgcccccga
     2821 cggccagccg gagaactcca ttgtctggtg ctcgtgggac ggcacgcacg tcgtcgtcaa
     2881 ttcgaccgac agccggcgca agacgaagaa cgccacccgc aacccgctgg tggccctgac
     2941 ggccgtcgac cccaaagacc cattccactg gatcgacgtg cgcggctacg ttgaggagat
     3001 cgtccccgac ccggagtaca agaccatcga cgcccacgcc aaactctact gcggcgtgga
     3061 gcactattac ggcgactacg cccccatcga gcgccgcggc acggagaacc gggtcgatct
     3121 gaagatccgg ccgacgcggg ttgtcgccta ctaggccatg ccgaacggtg cgctgcccga
     3181 caagcccccc ggcgaactgg cccgcgccat catcggcgcg gagtacgcct acgtcgccca
     3241 gacggccttc caggccaacg aggaccgcgc ccgcgcctgg caatacttct tcgtcacctt
     3301 cgccacgctc atcgccgcgc tgctgtccac tcagatcgag agcatcgacc ggcggcagct
     3361 ctacctgacc ttcgtggtca tcttcgccct gctggccggg ctggggctga tcaccatcct
     3421 gcaactggtg cggctgcggc aggcgtggct ggagagcgtg caggccatga accagatcaa
     3481 ggcccggctg atggccgatg acccggcgct ggcggcctac ttccgctggc cggcggcaac
     3541 cgtgcccccg gcgttcaagc tgcgtagctt tggcttcttg caggcggtgt cggtggcgtt
     3601 gctgagcgga ttggccgtcg gcgcggccat tgcctttgcg gccctggccg gcgggcaggc
     3661 ggttgtgccc tgggccgtca gtctgctgat cggcggcgcg tccgccgcgg gcattctgtg
     3721 gctgggctac gtccggccgt tgcagcggat gaaaacgtaa gttgacgcga cagccagggg
     3781 cggtatgatc gcctcagccg gcttccagtc cggttgattc acgccatccc ttgccatagg
     3841 agccaccatg cgccgcctca tcgctgcctt cgtctccgct ttgctatttg ctgttggttg
     3901 cacgtcccag ccgtcggccg acagcgtcgc cacggccatt gtcctgaccc aacaggccca
     3961 acccacggcc accccattgc ccgaaccgac ggcgacgccc acgcctctcc ccacagccac
     4021 agccacgccc gcgcccctga cgccgaccgg catcttcgag agcctgtcgc cggccgtcgt
     4081 gcgcatcgag gcggacagcg gctttggcag cggcgtgctg atcgagggca actacatcct
     4141 gaccaacgcc catgtcgtct ggccggccca ggacgccatc gtcaccttcc ccgacggcac
     4201 aacgctggca gccgtgccgg tcgttggcgt agacctgctg gccgacctgg ccgtgctggg
     4261 gccggtcgag acggacgtcg atccactggc gctgtccgac aaccaggaac tggcgatcgg
     4321 tgacacgttg ttgctgatcg gctatccggg cgacgtgacc agcgctccga agccgacgct
     4381 gaccgagacg ctcatctcgc gtctgcgcga gcaggagaca tttgacatca cctatttcca
     4441 ggtgagtgcc cctgtcgccg gcggtcagag cggcggcatc gccgtgacga tggacggcga
     4501 agtggtcggc ctgaccggca atcgcattac cgaggcgcag tatggcctca tcgcggcgat
     4561 ggccgacatc cggtcgcgcg tggatagcat tgtcgccggc cagggcgagc cggccagcgg
     4621 gcgcggcccg gacctgttgg aagctgcgga caggcacttc gttcgccttc accgcacgga
     4681 cgagatgcgc atattcactg ctgacctgga caagggcaca gaactggagg tgacagtcga
     4741 cggcaacggc gacgcgctga tgcagcttct cggccccacg cccgacgaca gcgccctggc
     4801 caacgagacc agtcacggta ttgagacga
//
LOCUS       DAOSMQ010000233         4800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17775_length_4800_cov_3.256902, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000233 DAOSMQ010000000
VERSION     DAOSMQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17775_length_4800_cov_3.256902"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK829_08745"
     CDS             complement(<1..276)
                     /locus_tag="PK829_08745"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HOU41342.1"
                     /translation="MNEDSSRPKNRRLLRVTALALTPLLLLAALLLVTLQTSASSHRE
                     APLISKDPFADNTDTYVWIPAGQTRNIVLAASWIPFEGPEGGPNYFEW"
     gene            complement(577..>1422)
                     /locus_tag="PK829_08750"
     CDS             complement(577..>1422)
                     /locus_tag="PK829_08750"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOU41343.1"
                     /translation="DALTDEERAEVDAYVAANPAARARLSAALAGAAALVELSTPLAP
                     APEVKARLMARVEADAARATDDRRPTAGDQRPAPPARRPAAPAGRRFGLRDFFFGRAL
                     GFAVLLLLLGAFGLWRLWSQSNDLQARVDTLEQSNAALQAQLDQARSVNTLLLDELAG
                     RDETLAHLTQPGAVTFALSDPSGARPAAAGTVTTTADGAVTLAVMNLPPPEADQTYQA
                     WLIVGQTPISAGTFAVDATGRALVTMPNPPAGAFDAFGVSLEPAGGSETPTPDQIVLL
                     GSRTS"
     assembly_gap    1423..1522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1686..2351
                     /locus_tag="PK829_08755"
     CDS             1686..2351
                     /locus_tag="PK829_08755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU41344.1"
                     /translation="MHLYLIRHGQSYVNLKDWSGGNQDTGLTDLGHRQAAALGQWMPT
                     EIISIDALYASTMHRAQETAAYVAAAYGVAITNDDRLREIGNNRADHLPWPSDDLPEY
                     SDYWGSERPFASVTPGREGGESLMHFRTRVGAFVEDMVERHRNQVVVAVCHGGVIELS
                     FDHIFNIGPWRRCEVWCKNTGVTRFEYVEHPRREMWRLHYHSRAEHLQGIESAAASLT
                     LQE"
     gene            2362..3855
                     /locus_tag="PK829_08760"
     CDS             2362..3855
                     /locus_tag="PK829_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOU41345.1"
                     /translation="MQREPSTAAVAVERDYSRKWLVMSAVAMGIFLATIDGSIVSVAL
                     PTLVIDLRTEFAIVQWVVLAYLLTLTTLLPVVGRLADMYGRKQLYLPGFVVFTIGSLL
                     CGLSPTVGWLIGARVLQGVGAALILALGLAIVTDAFPPGERGRALGVAGTIVSIGIVA
                     GPTLGGIIIENLSWHWIFFVNVPIGFLGVYLTLRHVPLARPPGNQRFDLAGAATLCLA
                     LLGILLSLTTAQQSGFTDRIAIMQFVFGVFFLVAFIVIQRRHPQPIIDLELFRNPVFS
                     VNLATGFLVFIGQGSAILIPFYLEEVLGYNVQQVGLLLVVLPLAMGIVAPISGTLSDR
                     MGSRPITVAGLAVIALGYAVMATLGTETTAVGFMWRYALIGVGIGLFNSPNNSATMGT
                     APPNRLGVASALMSLTRSMGQTMGLALLGALWAAVIFRDAGGILAGGATTAPPAYQAA
                     ALRVVMLAVAGTDVLALALALVAWRGERGQPVLPRAELEQETIQIGH"
     gene            complement(3808..4278)
                     /locus_tag="PK829_08765"
     CDS             complement(3808..4278)
                     /locus_tag="PK829_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41346.1"
                     /translation="MGEQGLSAIRSTLTNQIALIDGLLTRVLERAARIESGDPGHVES
                     TAFQVNNYYSVVEDLLRIVAAAFENNITDVSRWHSELIDRMALDIPGVRPPLLSTTTA
                     ARLHRLRSFRHFFRHAYRVDLDAGEVMDNVERVRDVHPHLVADLDRFLLQLGAG"
     gene            complement(4260..4610)
                     /locus_tag="PK829_08770"
     CDS             complement(4260..4610)
                     /locus_tag="PK829_08770"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOU41347.1"
                     /translation="MPYDTSVLDRALAERRAEWERRRQAMLARVLAVLDEWLPRFGVR
                     EAYIFGSLAQPGRYHAESDIDIAVVWEDRRDFFGMAGEVSRALGQDIDILPLEKIPFA
                     DKIRREGLRWVSKV"
BASE COUNT          759 a         1531 c         1577 g          833 t
ORIGIN      
        1 ccactcgaag tagttcggcc caccctctgg cccctcaaag ggaatccaac tggcggccag
       61 gacgatattg cgtgtttggc ccgccggaat ccagacgtag gtgtcggtat tgtcggcgaa
      121 cgggtctttg gagatgagcg gggcttccct gtggctcgac gcactggttt ggagggtaac
      181 cagcagcagt gcggccagca gcaacagcgg cgtcagcgcc agagcggtaa cgcgcaacag
      241 acggcgattc ttcggtcgag agctatcttc gttcacggtt gtctccttgt gcacaaactc
      301 ggaattaggt cgggtcgatc gagagcaaag agaaacgatt acaccaacat agtaggcctc
      361 cttcgggagc tagaacttgc atatgcttga tctcaattgg ataggtcgtc gcgatttgcg
      421 tcttgttggc gatgactaac catgattacg cagtggccaa tgagttgcat tcactagatt
      481 tgagaattgc cgtgtgggtt gcctaatgaa cacaaaaaaa ggacacggag ccgatgctgc
      541 cgtgtcctta aagcgcctgc aacgaggcgt taagcgttat gaagtccggc tgcccagcag
      601 gacaatctgg tcgggggtcg gcgtctcgct gccgccggcc ggctccagcg agacgccaaa
      661 ggcgtcgaac gcgccggccg gcgggttggg catggtcacg agcgcccgac cggtggcgtc
      721 cacggcgaac gtgcccgcgc tgatgggcgt ctggccgacg atgagccagg cctggtaggt
      781 ctggtcggct tccgggggcg gcagattcat gaccgccagc gtcaccgcgc cgtcggccgt
      841 cgtcgtcacc gtgccggcgg cggccgggcg cgcgccgctg gggtcgctca gggcgaaggt
      901 gaccgcgcca ggctgggtca ggtgggccag cgtctcgtcg cggccggcca gctcgtctag
      961 cagcagcgtg ttgaccgagc gcgcctggtc tagctgggcc tgaagcgccg cgttgctctg
     1021 ctccagcgta tccacgcggg cctgcaggtc gttggactgg ctccacagcc gccacagacc
     1081 aaacgcgccc aacagcagca gcaggacggc aaagcccagc gcccggccga agaagaaatc
     1141 acgcaggcca aagcgccgcc ccgccggcgc ggccggccgg cgggcgggcg gcgccggccg
     1201 ttggtcgccg gccgtcggtc gccggtcgtc ggtcgccctc gccgcgtccg cctcaacgcg
     1261 ggccatcagc cgcgccttca cctccggcgc gggggccagc ggcgtgctaa gttcgaccag
     1321 cgccgccgcg ccggccagcg ccgcgctcag ccgggcgcgc gccgccgggt tggcggccac
     1381 ataggcatct acctctgccc gctcttcgtc cgtcagggcg tcnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nngggcagta tatgggcctg gccatcgcca tcggcgcggc
     1561 gttgggcgtc gtctttggcc agatgaaagg caatctggcc ctgggcctgg ctttgggcac
     1621 agcgctagga gtggccgtcg ggtcgtttct cgacgcgcgc cgctaaccgg ccgcaaggaa
     1681 gtgttatgca tctctacttg atccgtcacg ggcaaagtta tgtcaacctc aaggactgga
     1741 gcgggggcaa tcaagacacc ggcctgaccg acctgggcca ccggcaggcc gccgctctgg
     1801 gccagtggat gccgacggag atcatcagca tcgacgcgct ctacgccagc accatgcacc
     1861 gggcacagga gacggccgcc tatgtcgccg cggcctacgg cgtggccatc accaacgacg
     1921 accgcctgcg cgagatcggc aacaaccgcg ccgaccattt gccctggccg agcgacgacc
     1981 tgccggagta tagcgattac tggggcagcg agcggccctt tgcgtccgtc acgccggggc
     2041 gcgaaggcgg cgagagcctg atgcacttcc gcacgcgcgt gggcgcgttc gtcgaggata
     2101 tggtcgagcg ccaccgcaat caggtcgtcg ttgccgtctg ccacggcggc gtcatcgagc
     2161 taagcttcga ccacatcttc aacatcggcc cgtggcggcg ctgcgaggta tggtgcaaga
     2221 acaccggcgt cacccgcttc gaatacgtcg agcacccacg ccgcgagatg tggcggttgc
     2281 actatcacag ccgcgccgaa cacctccagg gcatcgagtc ggccgcggcg tcgctgacgc
     2341 tgcaagagta ggatgtcggc tatgcagaga gagccttcaa ccgcggctgt cgctgtggaa
     2401 cgcgactata gccgcaaatg gttggtcatg tcggccgtgg ccatgggtat cttcctggcc
     2461 accatcgacg gcagcatcgt cagcgtcgcc ctgccaacgc tggtcatcga cctgcgcacc
     2521 gagtttgcca ttgtgcaatg ggtcgtgctg gcctatctgc tgacgttgac gacgctgtta
     2581 cccgtcgtcg gccggctggc ggacatgtac ggccgcaagc agctctacct gcccggcttc
     2641 gtcgtcttca ccatcggctc gctgctgtgc gggctgtcgc cgacggtcgg ctggctcatc
     2701 ggcgcgcgcg tcctgcaagg cgtcggcgcg gcgctgatcc tggcgctggg cctggccatc
     2761 gtcaccgacg ccttcccgcc gggcgagcgc ggccgggctt tgggcgtggc cggcaccatc
     2821 gtctccatcg gcattgtcgc cggcccgacg ctgggcggca tcatcatcga aaatctgtcg
     2881 tggcactgga tcttcttcgt caacgtgccc atcggcttcc tgggcgtcta tctgacactg
     2941 cgccacgtgc ccctggcgcg gccgccgggc aaccagcgct tcgacctggc cggcgcggcg
     3001 acgctgtgcc tggccctgct gggcatcctg ctgagcctga cgacggccca acagagcggc
     3061 ttcaccgatc ggatagcgat catgcaattc gtctttggcg tcttcttcct cgtcgcgttc
     3121 atcgtcatcc agcgccgcca cccgcagccg atcatcgacc tggagctatt ccgcaacccc
     3181 gttttctccg tcaacctggc caccggcttc ctggtcttca tcggccaggg gtcggccatc
     3241 ctcatcccgt tctacctgga agaggtgctg ggctacaacg tgcagcaagt cggcctgttg
     3301 ctggtggtct tgccgctggc catgggcatc gtcgcgccca tttccggcac cctgagcgac
     3361 cggatgggca gccggccgat caccgtagcg ggcctggccg tcatcgctct ggggtatgcg
     3421 gtcatggcga cgctgggcac ggagacgacg gccgtcggtt tcatgtggcg ctacgcgctt
     3481 atcggggtcg gcatcggcct gttcaactcg cccaacaaca gcgccacgat gggcaccgcg
     3541 ccgcccaacc ggctgggcgt ggcctcggcg ctgatgtcgc tgacgcggtc gatggggcag
     3601 acgatggggc tggcgctact gggggcgctg tgggcggcgg tcatcttccg ggacgcgggc
     3661 ggcatcctgg ccggcggggc cacgaccgcg ccgccggcct atcaggcggc ggcgctgcgc
     3721 gtggtcatgc tggccgtcgc cggcacagac gtgctggccc tggcgctggc gctggtggcc
     3781 tggcgcggcg agcgcggcca gccggtgcta ccccgcgccg agttggagca ggaaacgatc
     3841 cagatcggcc actagatggg ggtgaacgtc gcgcacgcgc tcgacgttat ccatgacctc
     3901 gccggcatcg agatcaaccc gataggcgtg gcgaaagaag tggcggaagc tgcgcaggcg
     3961 atgcagccgc gccgctgtcg tggttgacaa taggggcggg cgcactccgg gaatgtccag
     4021 ggccatgcgg tcgatgagtt cgctatgcca acgagagaca tcggtgatgt tgttctcaaa
     4081 cgcggcggcc acgatgcgta gcaggtcttc aacgacgctg tagtagttgt tgacctgaaa
     4141 cgccgtgctc tcgacgtgac cggggtcgcc cgactcgatg cgcgcggccc tttcgagaac
     4201 cctcgtcagc agtccgtcaa tgagcgcgat ctggtttgtc agcgtggaac gtatcgcgct
     4261 cagaccttgc tcacccatcg tagcccctcc cgtctgatct tgtcggcaaa cggaatcttt
     4321 tccagtggca ggatgtcgat gtcctggccc agcgcacggg acacttcacc ggccatgccg
     4381 aagaagtcgc ggcgatcctc ccagacgacg gcgatgtcga tgtcggattc ggcgtggtag
     4441 cgccccggtt gggccagcga gccgaagatg tacgcttccc gcacgccgaa gcgcggcagc
     4501 cattcgtcca gcaccgccag caccctggcc agcatggcct gtcgccggcg ttcccattcg
     4561 gcgcggcgct cggccagggc gcggtcgaga acggaggtgt cgtagggcat ggccacatta
     4621 tagcacaggc gtgtaggacg ggttggcaac ccgtctctgg ggacggggct tttcagttgt
     4681 cgccaattga ctcatgacct gacaggtggg cggccccaag cctcgtcgct atcaggctac
     4741 cgttcgccgc ccacctagta tgtccctata ggggtagaat aggaagggaa acaggatggg
//
LOCUS       DAOSMQ010000234         4799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17776_length_4799_cov_4.584317, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000234 DAOSMQ010000000
VERSION     DAOSMQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17776_length_4799_cov_4.584317"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK829_08775"
     CDS             complement(<1..364)
                     /locus_tag="PK829_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOU41348.1"
                     /translation="MLVGVPKEIKDNEYRVAMTPAGVQQLIEQGHTVLVETKAGDGSR
                     FPDEQYARVGAKIVPTATDAWGAEMVVKVKEPIAVEYPFLRSDLVLFTYLHLAAEEKL
                     TKAMLDSGVTGIAYETVEA"
     gene            complement(592..1584)
                     /locus_tag="PK829_08780"
     CDS             complement(592..1584)
                     /locus_tag="PK829_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HOU41349.1"
                     /translation="MELLIIGGTRFLGRALVDEARVAGHTVTLFNRGRSNPALYPDVE
                     QLHGDRDGGLAALAGRRWDAAIDTCGYVPRVVRAAAEFLAPAIDHYTFISTMSVYAEP
                     LAEGTDEAAPVGTIPDESIEEITGGSYGPLKALCERAATEAMDGRALHVRSGLIVGPH
                     DASDRFSYWPARVARGGEVLAPVSPDYGVQFIDVRDLAAWTLRATAARLTGAYNVTGP
                     ERPLPIGRLLDAARDLTGSDARFTWVPDDFLTAHDVGPYVELPLWVPADAGGFNSFNI
                     NRALAAGLTFRPVTETIADTLAWLATRPDDTVWRAGLTAEREAQLLAEWHTANG"
     gene            1743..3233
                     /locus_tag="PK829_08785"
     CDS             1743..3233
                     /locus_tag="PK829_08785"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOU41350.1"
                     /translation="MEIHVQQGSIESGAADTIIVNLFEGVTTPGGATGAVDQALGGAI
                     SDLIAGGDLKGKAGEVAVLYPRGAIAARRVLVVGLGKAESFDLEGIRRASAAAVKRAR
                     DLNARQVATIVHGAGIGGQDATAATQATVEGALLATYRYDAPKKRESQNDIDTLTIVE
                     RDAGRLEAVRAGAALGEAVAAGVTLARDLVNMPPNVATPTLLAERARAMATAHGLGVT
                     VGDRAWAAERQMGAFLAVAQGAGEPPAFIILEHNAGRADLPTVVLVGKGITFDTGGIS
                     IKPSAGMGDMKSDMGGAAAVFGAMEVVGRLGLPLHVVGITPCTENMPDAAAYRPADVI
                     TASNGKTIEIISTDAEGRMVLADALVHAGQYQPSAVIDLATLTGACVVALGEGVAAGL
                     FCNDDALRDQLLAAADATYERLWPLPLYEDYRRTINSDVADMKNSGGRSGGVGTSAVF
                     LREFTDYPWAHLDIAGMALLAKVGDYAPAGGTGFGVRLLVEYLRRL"
     gene            3291..4448
                     /locus_tag="PK829_08790"
     CDS             3291..4448
                     /locus_tag="PK829_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HOU41351.1"
                     /translation="MAVPKQPLTLGIEEEYQIIDPETRDLRTYISELLTQDQARARRL
                     DLKPELMQSQVEVGSHICRNIKEARAEIVRLRREVLELAAENGLLIAAASTHPFARWE
                     DQIITEGTRYKELLDDMQGVARQLLIFGMHVHVGFGSDPESRELLIATMNQARYFIPH
                     LLALSTSSPFWRGQNTGLKSYRSVVFQSLPRTGIPHSFISWADYKDYETMLERVGAFG
                     KLDKRAKIWWDIRPHPIFPTMEFRIADICTSVDDCICIAALFQAICAKLLKLRQQNMT
                     WRQYRHVHITENKWRAVRYGIDGELIDFGIQQSVPLPHLVGELVELVDDVVDDLDSRQ
                     EVAHVFNILRDGTSADRQLRVYREHGGDDNCEEALRAVVDHLVAETKRGVL"
     gene            complement(4521..>4799)
                     /locus_tag="PK829_08795"
     CDS             complement(4521..>4799)
                     /locus_tag="PK829_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU41352.1"
                     /translation="DRHSGSGCADGRPGPDGWYPEQRLTLGQALYAFTGAAALTSGQS
                     AQQGSIAPGKLADLTIFDRDPFAAPADELLSMSIAATMVGGQFRYRRI"
BASE COUNT          804 a         1589 c         1641 g          765 t
ORIGIN      
        1 gcgcttcgac ggtttcatag gcgatgccgg tgacgccgga atcgagcatc gccttggtga
       61 gcttttcctc cgcggccagg tgtagatagg tgaacaacac caggtcagag cgcaggaagg
      121 ggtactccac ggcgatgggt tctttgacct tgacgaccat ctccgcgccc caggcgtcgg
      181 tcgcggtggg cacgatcttg gccccgacgc gcgcgtactg ctcatcggga aagcggctgc
      241 catcgccggc cttagtctcg accagcacgg tgtggccctg ctcgatgagc tgctgcacac
      301 ccgcgggcgt catggccaca cggtattcgt tatccttgat ctcttttggt acgccgacta
      361 acatgcgaca ctccttagga aatgggtggg taggaatatt gtactgcaat cagcgcgacg
      421 gcctagcgat gacagggttt tcccaaagtc gtcgagcaac cattgacttg acagttgggc
      481 ggccagtcgt tctctggtgg cagtcccatg cgtggcccct acctgtcagg tcggcgcgcc
      541 gggggtgact ttggcaaagc cctgcctggc gatgacaagg gtttgccgtt gtcagccgtt
      601 agcagtgtgc cactcggcca gcaattgcgc ctcgcgttcg gccgtcaggc cggcccgcca
      661 gactgtatca tccgggcgcg tcgccagcca ggccagcgtg tcggcgatgg tctccgttac
      721 cggccggaag gtcagccccg cggccagcgc ccggttgatg ttgaagctgt tgaagccgcc
      781 ggcgtcggcc ggaacccaca gcggcaactc cacgtagggg ccaacgtcat gggccgtcag
      841 aaagtcatcc ggcacccagg taaagcgggc gtcgctgccg gtcaggtcgc gggcggcgtc
      901 gagcaggcgg ccgatgggca gaggccgctc cggcccggtg acgttgtaag caccggtcag
      961 ccgcgccgcg gtggcccgca acgtccacgc ggccaggtcg cgcacgtcga tgaactgcac
     1021 gccataatcg ggcgagaccg gggccagcac ctcgccgccg cgggcgacgc gcgccggcca
     1081 gtagctgaag cggtcgctgg cgtcgtgggg gccgacgatc aggccggagc gcacgtgcag
     1141 ggcgcggccg tccatcgcct ccgtggccgc ccgctcgcac agggccttca gcgggccata
     1201 cgatccgccg gtgatctctt cgatgctctc gtcgggaatc gtccccaccg gggcggcttc
     1261 gtccgtgccc tcggccagcg gttcggcgta gacggacatg gtggagatga aggtgtagtg
     1321 atcgatggcc ggggccagga attcggccgc ggcgcgcacc acgcggggca cgtagccgca
     1381 ggtatcaatg gccgcgtccc agcgccgccc ggccagcgcg gccaggccgc cgtcgcggtc
     1441 gccgtggagt tgctccacgt cggggtagag ggccgggttg gagcgcccgc ggttgaacag
     1501 cgtcaccgtg tggccggcga cgcgggcctc atcgaccaga gcacgaccga gaaagcgcgt
     1561 gccgccgata atcagcaact ccatgtggta tcaacctcct cgcggatgtg gcggcgtggg
     1621 tgtgtccgcg cgccccaccg gggaggatag cgctgccgtg gttcattgtc gagtaccgca
     1681 caaggggtag aattgccgcc aatgaaaaga cgccgcagca tcggcggcct aaggagcaaa
     1741 cgatggaaat ccatgtgcaa caaggcagta tcgagtccgg cgcggccgat acgatcatcg
     1801 tcaacctgtt cgagggcgtc acgacgcccg gcggggcgac tggcgcggtc gatcaggccc
     1861 tcggtggggc catcagcgac ctcattgccg gcggcgacct gaagggcaag gccggcgaag
     1921 tggccgtgct ctacccgcgc ggggccattg ccgcccggcg cgtgctcgtc gttgggctgg
     1981 gcaaggcgga gtcgttcgac ctggagggca tccggcgggc gtcggccgcg gcggtcaagc
     2041 gcgcccgcga cctgaacgcc cggcaggtgg ccaccatcgt ccacggcgcg ggcattggcg
     2101 gccaggatgc caccgcggcg acacaggcga ctgtcgaagg ggccttgctg gccacctatc
     2161 gctatgacgc ccccaagaag cgcgaatcac agaacgacat cgacacgctg accatcgtcg
     2221 agcgcgacgc cggccgcctg gaggccgttc gcgccggcgc ggcgctgggc gaggccgtgg
     2281 ccgcgggcgt gacgctggcc cgcgacctgg tcaacatgcc gcccaacgtg gccacgccga
     2341 cgctcctggc cgagcgggcg cgggcgatgg cgacggctca tggtctgggc gtcaccgtcg
     2401 gtgaccgggc ctgggcagcc gagcgccaga tgggggcgtt cctggccgtg gcccagggcg
     2461 cgggcgagcc gccggcgttc atcatcctgg agcacaacgc cggccgcgcc gacctgccga
     2521 ccgtcgtcct cgtcggcaag ggcatcacct tcgacaccgg cggcatttcc atcaagccca
     2581 gcgcgggcat gggcgacatg aagagcgaca tgggcggggc ggcggccgtc ttcggggcga
     2641 tggaagtcgt cggccggttg gggctgcccc tgcacgtcgt cggcatcacc ccctgcaccg
     2701 agaacatgcc cgacgccgcg gcctatcgcc cggccgacgt catcaccgcc agcaacggca
     2761 aaacgattga gatcatcagc accgacgccg aagggcggat ggtgttggcc gacgcgctgg
     2821 tacatgccgg gcaataccag cccagcgcgg tcattgacct ggccacgctg acgggcgcgt
     2881 gcgtcgtggc tctgggcgag ggcgtggccg ccgggctgtt ctgcaacgac gacgctttgc
     2941 gcgaccagtt gctggccgct gccgatgcga cctacgagcg cctttggccc ctgccactct
     3001 acgaggacta ccgccggacg atcaactccg atgtggccga catgaagaac agcggcgggc
     3061 gcagcggtgg cgtgggcacg tcggccgttt tcctgcgcga gttcaccgac tacccctggg
     3121 cacacctgga catcgccggc atggccctgt tggcgaaggt cggcgactac gcccccgccg
     3181 gcggcacggg cttcggcgtg cgcctgctgg tcgagtattt gcgccgtctc tagacgccgg
     3241 ctcgcgccct gcctataatc cgcgatcact atgtgagcag gaggtagaag atggccgtcc
     3301 ctaagcaacc gctgaccctc ggcattgagg aagagtacca gatcatcgat ccggagacgc
     3361 gcgacctgcg cacctacatc agcgagttgc tgacccagga ccaggcgcgg gcgagaaggc
     3421 tagacctgaa gccggaactg atgcaatcac aggtcgaggt cggcagccac atctgtcgca
     3481 acatcaagga ggcgcgggcg gagatcgtcc gcctgcggcg cgaggtgctg gaactggccg
     3541 ccgagaacgg cctgctgatc gctgcggcct ccacccaccc gttcgcccgc tgggaagacc
     3601 agatcatcac tgagggcacg cgctacaagg aactgctgga cgacatgcag ggcgtcgccc
     3661 gccaattgct catcttcggc atgcacgtcc acgttggctt cggcagcgac ccggagtcgc
     3721 gcgaactgct catcgccacg atgaatcagg cgcgctactt cattccccat ctgctggccc
     3781 tgtcgaccag ttcgcctttc tggcgcggcc agaacaccgg cctgaagtcc tatcgcagcg
     3841 tcgtcttcca atccttgccg cgcacaggca tcccccactc gttcatctcc tgggccgatt
     3901 acaaggacta cgagacgatg ctggagcggg ttggcgcgtt cggcaagctg gacaagcggg
     3961 ccaaaatctg gtgggacatc cggccgcacc ccattttccc gacgatggag ttccgcatcg
     4021 ccgacatctg cacctcagtc gatgactgta tttgcatcgc tgccctgttt caggccattt
     4081 gcgccaagtt gctgaagctg cggcagcaga acatgacctg gcggcagtat cgccacgtcc
     4141 acatcaccga aaacaagtgg cgggcggtgc gctacggcat cgacggcgag ctgatcgact
     4201 tcggcatcca gcagtcggtg ccgctgcccc atctggtggg cgagctagtc gaattggtag
     4261 acgacgtcgt cgatgatctg gacagccgcc aggaagtggc ccacgtcttc aacatcttgc
     4321 gcgacggcac cagcgccgac cgccagctgc gcgtctatcg cgagcacggc ggcgatgaca
     4381 actgcgagga ggcgctgcgg gccgttgtcg atcatctggt ggccgagacc aagcgcggcg
     4441 ttctctagcg cccaggagcg attctccggc cgccggccga caagaggggg cggagcgggt
     4501 caacgactgc tccgcccctt ttagatccgt cgatagcgga actgaccgcc gaccatcgtc
     4561 gcagcgatac tcatactgag caactcgtcg gcgggcgcgg cgaaaggatc gcggtcgaag
     4621 atggtgagat cggccagttt gccgggggcg atgctgccct gctgggcgct ctgcccactg
     4681 gtcagcgccg ccgcgcccgt gaaggcgtag agcgcctgcc ccagcgtcag ccgctgctcc
     4741 ggataccagc cgtcggggcc ggggcggcca tcggcgcagc cggaaccgct gtggcggtc
//
LOCUS       DAOSMQ010000235         4770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_17903_length_4770_cov_4.315376, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000235 DAOSMQ010000000
VERSION     DAOSMQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17903_length_4770_cov_4.315376"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PK829_08800"
     CDS             <1..558
                     /locus_tag="PK829_08800"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOU41353.1"
                     /translation="QPLRVLDLSEEQLKGLRGYGLLTLKPKVVLINIGDDQNEIDPAY
                     NYPQTIVANIRGRLEMDLGQLSAAGDADSLALFMEEYGVGELGLDRVIHLSYDLMGLQ
                     SFFTVGEDEVRAWEVKRGATAVDAAGVIHTDLAKGFIRAEVVHYDDLVALGGLSQARN
                     TGKLRQEGKTYIVQDGDVINIKFNL"
     gene            755..1855
                     /locus_tag="PK829_08805"
     CDS             755..1855
                     /locus_tag="PK829_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU41354.1"
                     /translation="MFKRIFAEGVAGRRVWPLVLAIAALLALLTACGGNEPPDLVIPT
                     AAATTVAGQQTVTATAPAALPTHTLPPPPTAVLATATAVPPTAVPPTAVPPTAVPPTA
                     VPPTAVPPTATPTAVPPEPVAAAYRVAFVAANDALNIRRQPDSDSAVVGQLPPNATGI
                     VAAGEGQSVRGGGFWLPVDTSVGSGYVNSRFLTESIDGATFCADPAVGELLTRLQTAI
                     AAEDARQLNELVHPTRGLRLRLNWWNEEVLVREAEIKDIFKDRARDAVDWGVSEGSGQ
                     PVRGTFSEVMLPFLTKDLLGATQWTCDQIVHGPTTGMVILPEGYEAVHFYSAYRPAPA
                     GQEFDWGTWVVGVERWQGRYYVSYLIHYRYEI"
     assembly_gap    1989..2088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2174..2686
                     /locus_tag="PK829_08810"
     CDS             2174..2686
                     /locus_tag="PK829_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOU41355.1"
                     /translation="MKITTMEDLFAHDLQDLRSAEVQLVEALPKMAAAASNSELKRAF
                     TQHLKQTENHVARLDAALKQIGKGPGREKCKGMQGLIEEGESAIEDVTSPDILDAALI
                     GAAQRVEHYEIAGYGTARAHAEVLGMKDMVDLLQTTLDEEGEANKLLTQLADGRLAIG
                     INERAAAGSR"
     gene            complement(2824..>4770)
                     /locus_tag="PK829_08815"
     CDS             complement(2824..>4770)
                     /locus_tag="PK829_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOU41356.1"
                     /translation="PLPAESVPLSAANGRVTAAGVWARLSSPHYHAAAMDGYALRAAD
                     TVGALDAAPRHFALVAAGKTPASAGDPAGEAPPAQPVNTGHPLPPWANAVVMIEDVQP
                     VTLAGGRAGIALRAALPPWQHVRAMGEDMVATELVLPANHRLRPVDLGALAGAGHAAV
                     DVYRRPRVAILPTGSELVAVEAGEPSPGQIIEYNSLVLAAQVAEWGGEATRWPIVPDD
                     FDAIVAAVRAAAAGHDLILLNAGSSAGSEDYAAHVVAVLGRLLVHGVAVRPGHPVILG
                     MVRRQGDEMTANKTGEHRPPVVPIIGVPGYPVSAALTGDIFVEPLLARWRGQPAYQPP
                     TLQGTLTRKLSSHTGDEDYVRVVVGQVGERTTVTPISRGAGVITSLVRADGIVRSPRF
                     SEGEDAGATVTVHLYRDPREIARGLVAIGSHDLALDLLAQFLAERYEGLRLVSANVGS
                     LGGLTALRRGEAHLAGTHLLDPATGEYNTAYVRRYLPGQPVVLVTLAGREQGWMVPRG
                     NPKRLSGWADAARPDVTLVNRQRGAGTRVLLDYELGRLNIDPAAVRGYEREEYTHLAV
                     AAAVASGAADAGLGIQAAARALRLDFVPLAHEPYELAIPQPVYDSERLRPLLDLLAGD
                     AFRAAVAAMPGYDTAIMGARRLIA"
BASE COUNT          767 a         1588 c         1611 g          704 t
ORIGIN      
        1 cagccgctgc gcgtcctcga cctgagcgag gagcaactca agggactgcg cggctacggc
       61 ctcctgaccc tgaagcccaa ggtcgtgctg atcaacatcg gcgacgacca gaacgagatc
      121 gaccccgctt acaactaccc gcagaccatc gtcgccaaca tccgcggccg gctggagatg
      181 gacctgggcc aactcagcgc cgccggcgac gccgactcgc tggcgctgtt catggaggag
      241 tatggcgtgg gtgagttggg cctcgaccgg gtgatccatc tgagctatga cctgatgggc
      301 ctgcaatcgt tcttcaccgt cggcgaggac gaggtgcgcg cctgggaggt gaagcgcggg
      361 gccacggccg tcgacgccgc cggggtcatc cacaccgacc tggccaaggg cttcatccgc
      421 gccgaagtgg tgcattacga tgacctggtt gccctgggcg gtctgtccca ggcccgcaac
      481 accggcaagc tgcggcagga aggcaagacc tacatcgttc aggacggcga cgtcatcaac
      541 atcaagttca atttgtagga ctggtagcca gcggtggacg gctggcctac gcgtgcccta
      601 cgcgcctttc gtcttcggaa cctctacgcc ttaggtagcg tcacttcatt gtaagtaact
      661 ggtcgccggc cgcctgtggc cggcgagagg ctgcccgact ggatcgtcag tcgcaacccg
      721 gcagtcagtc gattatcttg aggcgagagg tcttatgttc aagcgtattt ttgcagaagg
      781 agtggccggg cggcgcgtct ggccattggt tctggccata gccgcgctgc tggcgctgct
      841 gacggcctgc ggcggcaatg agccgcccga tctggtcatc cccacggccg cggcgacgac
      901 cgtcgccggc caacagactg tgacggcaac tgcccccgcg gcgctgccca cccacaccct
      961 gcccccgccg ccaacggcgg tgctggccac ggcgacggcc gtgccgccca cggctgtgcc
     1021 gcccacggcc gtgccgccca ccgccgtgcc gcccacggct gtgccgccca cggctgtacc
     1081 gcccacggcg acgcccaccg ccgtgccccc ggagccggtc gccgcggcct atcgcgttgc
     1141 ctttgtggct gccaacgacg ccctcaacat ccgccgtcag cccgacagcg actcggccgt
     1201 cgtcggtcag ttgccgccga acgcgactgg tatcgtcgct gccggcgagg gacagtccgt
     1261 gcgcggcggc ggtttctggt tgccggtaga cacgtctgtg ggcagcggct acgtcaatag
     1321 ccgctttctg accgagagca ttgatggggc gacgttctgc gccgacccgg ccgtgggcga
     1381 gttgttgacc cgcctgcaaa cggccatcgc ggccgaggac gcccggcagc tgaacgaact
     1441 agtgcaccct acgcgcggcc tgcgcctgcg gctcaactgg tggaacgagg aggtgctcgt
     1501 ccgcgaggct gagatcaagg acatctttaa agatcgggcg cgtgacgccg tcgactgggg
     1561 cgtgagcgag ggcagcggcc agccagtcag gggcacgttc agcgaggtga tgctgccctt
     1621 cctgaccaaa gatctgctgg gggcaacgca gtggacatgc gaccagatcg ttcacggccc
     1681 gacgacggga atggtgatct tgccggaggg gtatgaggcc gtccacttct atagtgctta
     1741 tcggcccgcg ccggccgggc aggagttcga ctggggcacg tgggtcgtcg gcgtcgagcg
     1801 ctggcaggga cgctactatg tcagctacct gattcactac cgctacgaaa tctagcgccg
     1861 ccctagacct gtcaggtcgg ctaagtctgg caaaacggcg ggaatgtacc ctctcttata
     1921 ccccttcctt cccgtgggca catagatttt gccttcgcta ggccttaaac tgacaaacga
     1981 atcaaagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa acgaatcaaa
     2101 gagcgaaggc cggccattgt gccccgcttc gccgggcgat gacagtggcc ctatttccaa
     2161 ccggaggtat atgatgaaaa ttacgaccat ggaagatctg tttgcccatg acctgcaaga
     2221 cctgcgcagc gccgaggttc aacttgtgga agcgctgccg aagatggccg cggcggccag
     2281 caactctgaa ctgaagcggg ctttcacaca gcatctgaaa cagaccgaga atcacgtcgc
     2341 ccgtctcgac gcagccttga agcagattgg caaaggcccc ggccgtgaga agtgcaaggg
     2401 catgcagggc ctcatcgaag aaggtgagtc ggccatcgaa gatgtcacca gcccggacat
     2461 cctggatgcg gcgctcatcg gcgcggccca gcgcgtggag cactacgaga tcgccggcta
     2521 tggcacggcg cgcgcccacg ccgaagtgct gggcatgaag gatatggttg acctgctcca
     2581 gacgacattg gacgaggaag gcgaggccaa caagctgctg acgcaactgg ccgacggccg
     2641 cctggccatc ggcatcaacg agcgcgcggc cgcaggttcc cgttaagagg ggcgattgcc
     2701 cacccgcaga ataattcccg caagggagca gtaagaagct agaacgtcgg gcgcgtcagc
     2761 gctcagacgc cgtaacgcga ggatgcccaa ccgtgggcat cctcgcgttg gcgttgggcc
     2821 tgtctacgcg atcagtcgtc tcgcgcccat gatcgcggtg tcgtagccgg gcatggccgc
     2881 tacggcagcg cggaaggcgt cgccggccag caagtccagc agcgggcgca gccgctcact
     2941 gtcgtagacc ggctggggga tggccagttc atacggctca tgggccaggg gcacgaagtc
     3001 gagccgcagc gcccgcgccg cggcctggat gcccagcccc gcgtcggccg cgccggaggc
     3061 caccgccgcg gccacggcca gatgggtata ctcctcgcgc tcatagccgc gcacggccgc
     3121 cgggtcgatg ttcagccggc ccagttcgta gtccagcagc acccgcgtgc ccgcgccgcg
     3181 ctggcggttg accagggtca cgtcgggccg ggcggcgtcg gcccagccgc tcagcctctt
     3241 ggggttgccg cgcggcacca tccagccctg ctcgcggccg gccagcgtca ccaggacgac
     3301 gggctgcccg ggcagatagc gccggacgta ggccgtgttg tactcgcccg tcgccgggtc
     3361 cagcagatgc gtgcccgcca gatgggcctc gccgcgccgt agcgccgtca gcccaccgag
     3421 gctgccgacg ttggccgaga cgagccgcag cccctcgtag cgctcggcca ggaattgcgc
     3481 cagcagatcg agggccaggt cgtgactgcc gatggccacc aggccgcggg cgatctcgcg
     3541 tgggtcgcgg tagaggtgga cggtgacggt cgctccggca tcctcgccct cgctgaaacg
     3601 ggggctgcgg acgatgccat cggcccgcac cagcgaggtg atcacccccg cgccgcggct
     3661 gatgggcgtc accgtcgttc gctcgcccac ctggccgacg acgacgcgca cgtagtcctc
     3721 gtcgccggtg tggctgctca gcttgcgcgt cagcgtcccc tggagcgttg gcggctggta
     3781 ggccggctgg ccgcgccaac gggccagcag cggctcgacg aagatgtccc cggtcagcgc
     3841 cgcgctgacc gggtagccag gcacgccgat gatgggcacg accggcggac ggtgctcccc
     3901 cgttttgttg gccgtcattt cgtcaccttg ccggcgaacc atgcccagaa tgaccggatg
     3961 gcccggccgg acggcgacgc catgcaccag cagccggccg aggacggcga cgacatgagc
     4021 ggcgtagtcc tcgctgccgg ccgaagagcc ggcgttgagc aggatgaggt cgtggccggc
     4081 tgccgcggcg cggacggcgg ccacgatggc gtcgaagtcg tcggggacga tgggccagcg
     4141 tgtcgcctcg ccgccccact cggccacctg cgccgccagc accagcgagt tgtactcgat
     4201 gatctggccc ggcgacggct cgcccgcctc cacggccacc aattccgagc cggtggggag
     4261 gatggccacg cgcggccggc ggtagacatc cacggcggcg tgccccgccc cggccagcgc
     4321 gcccagatcg accggccgca gccggtggtt agcgggcaag accagctcgg tggcgaccat
     4381 atcctcgccc atagcccgga cgtgctgcca cggcggcagg gcggcgcgca gggcgatgcc
     4441 cgcccggccg ccggccagcg tcaccggctg gacatcctcg atcatcacga cggcattggc
     4501 ccacggcggc agcgggtggc cggtgttaac cggctgggcg ggtggcgcct cgccggccgg
     4561 gtcgccggcg ctggccgggg tcttgccggc ggctacaagg gcgaagtggc gcggcgcggc
     4621 atccaacgcg cccacggtgt cggcggcgcg cagggcgtaa ccgtccatgg ccgcggcgtg
     4681 gtagtggggc gaggagaggc gcgcccagac gccggcggct gtcacccggc cgttggccgc
     4741 gctcaggggc acgctctcgg ccggcagcgg
//
LOCUS       DAOSMQ010000236         4733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18084_length_4733_cov_3.806114, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000236 DAOSMQ010000000
VERSION     DAOSMQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18084_length_4733_cov_3.806114"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK829_08820"
     CDS             complement(<1..196)
                     /locus_tag="PK829_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41357.1"
                     /translation="MTGLWAEFRHTLRRLRGQILGWGIGLALYGLAMGLMYDGIQNIT
                     GLDEMLASYPRELLAFFGDMA"
     gene            complement(209..1126)
                     /locus_tag="PK829_08825"
     CDS             complement(209..1126)
                     /locus_tag="PK829_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41358.1"
                     /translation="MDTQPALSISGLTKSYGRVPALRGVDLEVGRGEIFGFLGPNGAG
                     KTTTIRCLLDLIRPDGGTASVLGLDPQADAVAVRRRVGYLPGELSIEPNLTAERALRD
                     LAELRGGVEWAFVREVAGRLQLDLRTAVKNMSKGNKQKVGVVAALMGRPELLLLDEPT
                     SGLDPLMQQEVYRLLREARANGATVFFSSHIISEVEAIAERVAIIRDGRIAETLAPHD
                     LVSRSVRRVRVRFGAPVDTMGLAVLRGVTHLEIHGDGNAPGRAPAITLQIEGEMDGLV
                     KALAAYPVVDLETERPSLEEIFLTLYQES"
     assembly_gap    1162..1261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1402..2088)
                     /locus_tag="PK829_08830"
     CDS             complement(1402..2088)
                     /locus_tag="PK829_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOU41359.1"
                     /translation="MQIELLYRPAFSLGVIHLDGGEQVRVEAASMVSMSPGITLETAA
                     TGGFLKSISRAVLGGESFFQNTYLAPPGGGKIAVAPALTGDLMVLELHDETLLVQSGS
                     YLASALTITTDTKWSGAKTFFASEGLIMLRAQGTGPLLLSSYGAIHEMNLAAGESITI
                     DTGHLVAFTQDIGFQVRKIGGLKSTLFSGEGLVVDLTGPGRVLAQTRSTDQFLSWLIP
                     KLPHTSSSSS"
     gene            2335..3549
                     /locus_tag="PK829_08835"
     CDS             2335..3549
                     /locus_tag="PK829_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU41360.1"
                     /translation="MPDATPTKHAHLHPSDLRALARLLTDATAGVTDLVEAVHIGVAP
                     ADKPNGVAPLTYRTIRSMTRFLGSGIDAALAQLTPLLGSRDASPERRLALAALNGVMG
                     DYLSDSGNPLAETMTFRRDGAPLALTREALAAAYPSGDKLVVLVHGLCMNDLAWDAGG
                     LGATLAGNLGYTPLALDYNSGRHISTNGRDLADHLEALIGAWPRPVTELVLIGHSMGG
                     LVARSAGHAGAVAGHAWPARLRALVFLGAPHHGAPLERGGNWINVLMAATPYVAPFAR
                     LGQGRSAGITDLRHGNLLDEDWHGRDRFAHVPDSRTPMPLPPGVHCFAAAAMLGQSAD
                     SLRDRLVGDGLVPLDSALGRHADPARNLPFDETNSWIGYGMNHLDLLRRPEVAAQVEA
                     WLAALQDEASRL"
     gene            complement(3555..>4733)
                     /locus_tag="PK829_08840"
     CDS             complement(3555..>4733)
                     /locus_tag="PK829_08840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU41361.1"
                     /translation="HVQAVAFNYERKHWQVLDPVIAADLDYLAGVADGEVEITSRSLD
                     DQYWLVANVVDDGPARYYLYDRAAGTAEFLFTNRAELEGLPLAKMRPAVVPARDGLNL
                     VVYYTLPPDSDSDGDGIPDAPRPMVFLPHGGPWGRDAWGFHGWHQWLANRGYAVLSVN
                     FRSSTGFGKAFTNAGDFEWGGKILADQQDGVQWAIAQGIADPARVAVMGGSFGGFSTL
                     AGLTFYPEVFACRVDIVGPSNLITLLETIPPYWQPMIELFTTRVGDHRTEAGRALLRA
                     HSPLTYVDRIVRPLLIAQGANDPRVKQAESDQIVSAMQARGIPVAYALYPDEGHGFAR
                     PENNLSFAALAEAFLARCLGGRAEPIGDDLAGSSLTVPVGAEEVPGLAAALAKAGAAG
                     "
BASE COUNT          722 a         1652 c         1548 g          711 t
ORIGIN      
        1 cggccatgtc gccgaagaag gccagcagct cgcgcgggta actggccagc atttcgtcta
       61 gcccggtgat gttctggatg ccgtcgtaca tcaggcccat cgccagcccg tagagcgcca
      121 ggccgatgcc ccagccgagg atttgcccgc gcaggcggcg caacgtgtgc cggaattccg
      181 cccacagtcc tgtcatgtcc ctcgcctcct aactttcctg gtagagcgtc aggaagattt
      241 cctccagcga cggccgctcc gtctccagat cgacgaccgg gtaagcggcc agcgccttca
      301 ccagcccgtc catctcccct tcaatttgca gggtgatggc cggggcacgg ccgggcgcgt
      361 tgccgtcgcc gtggatctcc agatgggtca cgccgcgcag gacggccagc cccatcgtgt
      421 ccaccggcgc gccgaagcgc acccgtaccc gccgcaccga ccggctgacg aggtcatgcg
      481 gagccagcgt ctcggcgatg cggccgtcgc ggatgatggc cacgcgctcg gcgatggcct
      541 ccacctcgct gatgatgtgg gacgagaaga agaccgtcgc cccattcgcc cgcgcttccc
      601 gcagcagccg gtagacctcc tgctgcatca gcgggtccaa gcccgacgtc ggttcatcga
      661 gcagcagcaa ctccggccgg cccatcagcg cggcgacaac gccgaccttc tgcttgttgc
      721 ccttggacat gttcttgacc gccgtccgca agtccagttg cagccgcccg gccacctcgc
      781 gcacaaaggc ccactccacc ccgccgcgca gctcggccag gtcgcgcagg gcgcgctcgg
      841 cggtcaggtt cggctcgatg ctgagttcgc cgggcaggta gccgacgcgg cggcggacgg
      901 ccacggcgtc ggcctgtggg tcaagcccca ggacgcttgc cgtgcccccg tcggggcgaa
      961 tgaggtcgag caggcagcgg atggtggtgg tcttgcccgc gccgttgggg ccgaggaagc
     1021 cgaagatctc gccgcggccg acttccagat cgacgccgcg cagggccggg acccggccat
     1081 atgacttggt caggccgctg atgctcaatg ctggttgggt atccatgtgg gtgttgcctc
     1141 gcttgcgcca gtgatgatat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 ngggcagaca cgcaggtctg ccctctctgg ccagtagaag aggcaaggcc cagcgaagaa
     1321 cgctcgtccc ctggaacctg gcccctggcc cctggcccct ggcccctggc ccctggcccc
     1381 ttcttctagc ccccattcac cctagctgct gctgctgctc gtatgcggca gcttggggat
     1441 gagccagctc aggaattggt cagtcgagcg cgtctgggcc aggacgcggc cggggccggt
     1501 caggtcaacg accaggcctt cgccgctgaa cagggtggac ttcaggccgc cgatcttgcg
     1561 cacctggaag ccgatgtcct gggtgaaggc aaccagatgg ccggtgtcga tggtgattga
     1621 ttcgccggcg gccagattca tctcgtggat agccccatag gacgacaaca gcagtggccc
     1681 cgtgccctgc gcccgcagca tgatcaggcc ctcggaggcg aagaacgtct tggccccgct
     1741 ccatttggtg tcggtggtga tggtcagggc cgaggccagg taggagccgg actggacgag
     1801 cagggtctcg tcgtggagtt cgaggaccat caggtcgcca gtcagagccg gggccacagc
     1861 gatcttgccg ccgccgggcg gggccaggta ggtgttctgg aagaagcttt cgccgcccag
     1921 aacggcgcgg ctaatggact tgaggaagcc gccggtggcg gccgtctcca gggtgatgcc
     1981 cggcgacatg ctgaccatcg acgccgcctc gacgcgcacc tgttcaccgc cgtcgaggtg
     2041 gatgacgccc agcgagaagg ccggacggta gagaagttcg atctgcatgg ctgattctcc
     2101 ttccggttta acaaccggac tacgagcgac atgagcctca aaccggctca tcataacacc
     2161 ccgttgccag aaaccaaacc ggccattgac ctgacaggtg agtgcgccgg gggtgacttt
     2221 ggaaaagccc gggcaaccgt cggcgcagac ctgcgtggca gcccctccag gcaggacgca
     2281 cacgccggca cgtccgtacc ttgcgccggc cgtcaagtcc ggtaccatcc cctcatgccc
     2341 gacgcaacgc cgaccaaaca tgctcatctc cacccgtctg acctgcgcgc cctcgcccgg
     2401 ctgctgaccg acgccaccgc gggcgtgacc gacctggtgg aggccgtcca cattggcgtc
     2461 gccccggccg acaagcccaa cggtgtcgct cccctgacct accgcaccat ccgcagcatg
     2521 acgcgcttcc tgggcagcgg catcgacgcc gccctggcgc aactgacgcc cttgctcggc
     2581 tcgcgcgacg cctcgcccga acggcgtctc gccctggcgg cgctcaacgg ggtcatgggc
     2641 gattacctaa gcgacagcgg caacccgctg gccgagacga tgaccttccg ccgcgacggc
     2701 gcgccgctgg cgctgacgcg cgaggcgctg gccgcggcct atccgtcggg cgacaagctg
     2761 gtggtgcttg tccacggtct gtgcatgaac gacctcgcct gggacgcggg gggcctgggc
     2821 gcgacgctgg ccggtaacct cggctacacg ccgctggcgc ttgactacaa ctccggccgc
     2881 cacatctcca ccaacggccg cgacctggcc gaccatctgg aggcgctcat cggcgcgtgg
     2941 ccccggcccg tgacggagct tgtcctcatc ggccacagca tgggcgggct ggtggcccgc
     3001 agcgccggcc atgccggcgc ggtcgccggc cacgcctggc ccgcccggct gcgggcgctg
     3061 gtctttctgg gcgcgcccca ccacggcgcg cccctggagc gcggcggcaa ctggatcaac
     3121 gtcctcatgg cggcgacgcc ctacgtagcc ccttttgcgc ggctgggtca gggtcgcagc
     3181 gccggcatca ccgatctgcg ccacggcaat ttgctcgacg aagattggca cggccgcgac
     3241 cgcttcgccc acgtgcccga cagtcgcacg ccgatgcccc tgccgcccgg cgtgcattgc
     3301 tttgccgccg cggccatgct ggggcaatcg gcggactcgt tgcgcgaccg gctggtgggc
     3361 gatggcctgg tgcccctcga cagcgccctc ggccgccacg ccgacccagc ccgcaacctg
     3421 ccgttcgacg agacaaatag ctggatcggc tacggcatga atcacctcga cctgctgcgg
     3481 cgaccggaag tcgccgcgca ggtggaggcg tggctggccg cgctccaaga cgaggcgtcg
     3541 cgcctctgag gcgatcagcc agccgccccc gccttcgcca acgccgcggc caaccccggc
     3601 acttcctctg cccccaccgg cacggtcagg ctgctgccgg ccaggtcgtc gccaatcggc
     3661 tcggcccggc cgcccaggca acgggccagg aacgcctccg ccagggccgc gaacgacagg
     3721 ttgttctccg gccgggcgaa gccgtggccc tcgtcggggt aaagggcgta ggccaccggg
     3781 atgcccctgg cctgcatggc gctgacgatc tggtcactct cggcctgctt gacgcgcggg
     3841 tcattggccc cctgagcaat gagcagcggc cggacgatgc gatcgacgta ggtcagcggc
     3901 gagtgcgccc gcaacagcgc ccgtcccgct tcagtgcggt ggtcgcccac gcgcgttgtg
     3961 aacagctcaa tcatcggctg ccagtagggc gggatcgtct ccagcagggt gatcaggttc
     4021 gacgggccga cgatgtccac gcggcaggcg aagacctccg ggtaaaaggt cagcccggcc
     4081 agcgtcgaga agccgccgaa gctgccgccc atgacggcca cgcgggccgg gtcggcaatg
     4141 ccctgggcaa tggcccactg cacgccgtcc tgctggtcgg ccaggatttt gccgccccac
     4201 tcgaagtcgc cggcgttggt aaacgccttg ccgaagccgg tggacgagcg gaagttgacc
     4261 gacagcacgg catagccgcg gttggccagc cattgatgcc agccgtggaa gccccaggcg
     4321 tcgcgccccc atgggccgcc gtggggcagg aagaccatcg gccgcggcgc gtcggggatg
     4381 ccgtcgccgt cgctgtcgct gtccggcggt agagtgtaat agacgacgag gttcagcccg
     4441 tcgcgggcgg gcacaaccgc cgggcgcatt ttggccagcg gcagcccctc cagctcggcc
     4501 cggttggtga acaggaactc ggccgtcccc gccgcccggt cgtagaggta atagcgcgcc
     4561 gggccgtcat cgacgacgtt ggcgaccagc cagtactggt cgtcgagcga gcggctggtg
     4621 atctccacct cgccatcggc cacgccggcc agatagtcga ggtcggcggc aatgaccggg
     4681 tcgagcacct gccagtgctt gcgctcatag ttgaaggcca ccgcctgcac gtg
//
LOCUS       DAOSMQ010000237         4721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18164_length_4721_cov_3.471710, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000237 DAOSMQ010000000
VERSION     DAOSMQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18164_length_4721_cov_3.471710"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(616..1263)
                     /locus_tag="PK829_08845"
     CDS             complement(616..1263)
                     /locus_tag="PK829_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41362.1"
                     /translation="MGRQSKALPAIAGQAMTGKEQRARRALLPLPPLILAALILLLLP
                     LVARAHGIGTPQRLNVPSGPYWLSIWTDPDPLRADQTHIVVAVLEPETQEPIVTGVEV
                     MVQLRSLADGTVRTQAAVTDNTNRLLYAAEFTDHVSPGRWEASVLVSGERGVGDPVTF
                     EVEIGPARRVNWLWVGVGGLGAALVVWLLSAGRAGRRRVRGRPTLLIRGIPVAAR"
     gene            complement(1317..2792)
                     /locus_tag="PK829_08850"
     CDS             complement(1317..2792)
                     /locus_tag="PK829_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FesM"
                     /protein_id="HOU41363.1"
                     /translation="MSTPSNPIDPPLIRLEDVVLGRGAPPPRFDVLRAPLLGRLLKAR
                     NGRLLLQIPFLLVAVLLVYDGLTGPQLAPQNLATVTVWIHTRGLVIVAALLLGNVFCM
                     GCPFTLPRTLARRLSGRGRRWPRALRNKWLAVAAFVAFLFLYEWLDLWAGPRLTAAVI
                     IGYFAGAFVLEALFAESPFCKYICPLGTFNFVANTAAPTQITVHDRDVCRACPGKECV
                     NGSARVLGCGTELFPPAVRSNLDCVFCLDCARACPYDNVALAVRRPGQEWTAGAPGDG
                     VWPRRWDVSFLVLVFTFAAVSNAFGMVPPFYDAQAWLAGALGLTSEGARLALTFVALN
                     LALPAVAGLAAAALSRALVGRREPLRVAFSRFAPAFVPLAVAIWLAHYGFHFATGALV
                     IVPVLQNFLLAHGLTWLGVAPNWSLAAILPGEWLLPLQTAAVLAGFAAALFAGNRLAR
                     RDFDDPAAAVRAFVPWLLVLLALAVAALATFNLPMEMRGLS"
     gene            complement(2767..3069)
                     /locus_tag="PK829_08855"
     CDS             complement(2767..3069)
                     /locus_tag="PK829_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOU41364.1"
                     /translation="MQLTLSPDPDTLVVGPLRLDVTLRDASGAPIDGATDVSLRGDMS
                     HAGMEPVLATAAGQGNGIYRADFTWTMAGDWIVTVEATLPDGRLKVTQFEYAIQPN"
     gene            complement(3181..3657)
                     /locus_tag="PK829_08860"
     CDS             complement(3181..3657)
                     /locus_tag="PK829_08860"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOU41365.1"
                     /translation="MKAYTKHRWVLVLLALAAVLVACGGNDSAATTGNEVTVGDLAIV
                     DAWARPPAMAGGNGAAYFVIRNDGDTADRLTGVSSPLAIAEMHESVMAADGTMSMNPV
                     AGVDVPPGSTVAFERGGLHIMFMGVAEPPAVGETVPLTLVFQNAGEITLDVPVREE"
     gene            complement(3963..4661)
                     /locus_tag="PK829_08865"
     CDS             complement(3963..4661)
                     /locus_tag="PK829_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HOU41366.1"
                     /translation="MDTALDSRPADADFSADAPASPPNRLWLLLVVAVAALAVGALGG
                     YWLGRGRVPAADSVDAGFARDMSLHHEQAVVMAGLVYDRSDDPAIRTLAYDILTTQQG
                     QIGIMSGWLDAWGLAWTSSGPRMAWMGMPTEGLMPGMATAEQLAALRAAGGEAADVLF
                     LQLMIPHHQGGVAMAAAAAENADQPMVRTMAASIVEAQQLEIDYMRELLAAKGGAELT
                     PAPAEDGGSMPSMP"
BASE COUNT          903 a         1609 c         1522 g          687 t
ORIGIN      
        1 tgcatttcca gctcccccag cgccgccttc cacacggttt cgggagatag tgacaatgtg
       61 ttcattcctc ttccctcagc aagcggccgc ggccaggcgc acgacaacct cggatgaccc
      121 gcttatggtt tttccgaaag atagacagac caagagaggc gctacgacat tgaagggggc
      181 agagcgtcat tccctctaaa accgagacac ccaccccgaa gcatgatcag cggcgacagc
      241 agccgttact ggacagacaa ggccccggcg ccgtcgcgaa gtcgccatca gatgtccctc
      301 aatgtctgaa cattctaaca tgccccctcg ctagggacaa tgctcagagc ctgttattgg
      361 cttaaaagct ggggattttt taggaatcgg caacgttaag aaattcgtcc tcatcatggt
      421 ggggaagggg gagcttgccc ccattccaac aattaggccc ccacacatcc catacccccc
      481 atatatagcc gcagtttacc ataaccgccg cctctaacaa gaagtatggc cgcgttacca
      541 accccttacc ggactaccgg ctaataccgc cggacggcat tggcggcaac agcgccgccc
      601 attcccggcg aattgttacc gagcggccac cggaatgccc ctaatgagca gggtagggcg
      661 gccgcggaca cgccggcggc cggcacgccc ggccgacagc agccacacca ccagcgccgc
      721 gcccagcccg ccaacgccca cccacagcca attgacgcgc cgcgccgggc caatttctac
      781 ctcaaatgtc accgggtcgc ccacgccgcg ctcgccgctg accaacacgc ttgcctccca
      841 gcggccgggc gagacgtgat cggtgaattc ggcggcgtag agtaagcggt tggtgttgtc
      901 ggtcaccgcc gcttgtgtgc gaactgtgcc gtcggccagc gagcgcaatt gcaccatcac
      961 ctcgacgccg gtgacaatcg gctcctgcgt ctctggctcc agcacggcga cgacgatatg
     1021 ggtctggtct gcccgcagcg ggtcggggtc agtccagatg gacagccagt aggggccgct
     1081 gggcacgttg agccgttgcg gtgtgccgat gccatgggcg cgagccacca ggggcaggag
     1141 cagcaagatg agcgcggcga ggataagtgg gggaaggggg agaagggcac gccgcgcacg
     1201 ctgctctttg ccggtcatgg cctggccggc aatggccggg agagctttgc tttggcggcc
     1261 catagcctga tccgcgttcc tcagttccat tgatgccatc cgcgctttac ggagcctcag
     1321 gacaggccgc gcatctccat cggcaggtta aacgtggcca gggcggcgac ggccagggcc
     1381 agcagcacca acagccacgg cacgaaggcg cgcacggccg cggccggatc gtcgaagtcg
     1441 cgccgggcca ggcgattgcc ggcaaagagc gccgcggcga acccggccag gacagctgcc
     1501 gtttgcaacg gcagcagcca ctcgcccggc aggatagccg ccagcgacca gttaggcgcg
     1561 acccccagcc atgtcagccc gtgggccagc agaaagtttt gcagcacagg cacgatgacc
     1621 agcgcgccgg tggcgaagtg aaagccatag tgggccagcc agatagccac ggccagcggc
     1681 acgaaggcgg gcgcaaagcg gctgaaggcg acgcgcagcg gctcgcgccg cccgaccaac
     1741 gcgcggctca gcgccgcagc cgccagcccc gcgaccgccg gcagagccag gttcagcgca
     1801 acgaacgtca gcgccagccg cgcgccctcg ctcgtcagcc caagcgctcc ggccagccat
     1861 gcctgcgcgt cgtagaacgg cggcaccatg ccaaaggcgt tgctgaccgc cgcgaaggtg
     1921 aagaccagca ctaagaagga cacgtcccag cggcgcggcc agacaccatc gccgggcgcg
     1981 cccgcggtcc attcctggcc gggccggcgg acagccaggg caacgttgtc ataggggcag
     2041 gcacgggcgc agtcgaggca gaaaacgcag tcgaggttgc tgcgcaccgc cggcggaaat
     2101 agctccgtgc cacaaccgag cacgcgggcc gagccattga cgcactcctt gccagggcag
     2161 gcgcggcaca cgtcccggtc gtggacggtg atttgcgttg gcgcggccgt gttggccacg
     2221 aagttgaacg ttcccagagg gcagatgtac ttgcagaagg gggactcggc gaagagcgct
     2281 tccagcacaa acgcgccggc gaagtagcca atgatgaccg ccgccgtcag ccgcggcccg
     2341 gcccagaggt cgagccactc atagaggaaa aggaaggcga cgaaggcggc cacggccagc
     2401 cacttgttgc gcagcgcccg cggccagcgc cggccccggc cactcagccg ccgggccaac
     2461 gtgcgcggca gcgtgaaggg gcaacccatg cagaagacgt tacccagcaa cagcgccgcc
     2521 acaatcacca ggccgcgcgt gtggatccag accgtcactg tggccaggtt ctgtggggcc
     2581 agctgcggcc cggtcaggcc gtcgtagacc agcagcacag ccaccagcag aaaggggatt
     2641 tgcagcagca gccggccgtt acgcgccttc agcaagcggc ccagcagcgg cgcgcgcagc
     2701 acgtcgaagc gtggtggcgg cgcgccacgg cccaggacga catcctccag ccggatcagc
     2761 ggcgggtcaa ttgggttgga tggcgtactc aaactgggtc accttgagac gaccgtcggg
     2821 cagcgtggcc tcgacggtca caatccagtc accggccatc gtccaggtga aatcggccct
     2881 gtaaatgccg ttgccctgac cggcggccgt tgccagcacc ggctccatgc ccgcgtggct
     2941 catgtcgccg cgcagcgata cgtccgttgc cccgtcgatg ggcgcgccgc tggcgtcacg
     3001 tagcgtcacg tccagacgca acggcccgac aacgagcgtg tccggatcgg gcgagagggt
     3061 tagctgcacg tcgggggcct gatcggttgt ctccgcgcgg cggcaaccac cggcaagaag
     3121 aagcgcgagc gcgagcgcca cccaggcaag ccaacgagcc ttccgcaaag ccatggccgg
     3181 ctactcctca cgcacaggta catccagcgt gatctcgccc gcgttctgga agaccagcgt
     3241 cagcggcacg gtctcgccca cggccggcgg ctcggccacg cccatgaaca tgatgtgcag
     3301 cccgccgcgc tcaaaggcga ccgtgctgcc gggcggaaca tcgacgccgg cgacgggatt
     3361 catcgacatc gtgccatcgg cggccatgac cgattcgtgc atctcggcta tcgccagtgg
     3421 gctggagacg ccggtgagac ggtcggccgt gtcgccatcg ttgcgaataa caaaataggc
     3481 cgcgccgttg ccgccggcca tcgccggcgg gcgcgcccag gcgtcgacaa tggcaagatc
     3541 gccaacagtg acctcgttgc cggtggtggc ggcgctgtcg ttgccgccac aggcgaccaa
     3601 cacggccgcc agggccaaca atacgagcac ccaacgatgc ttggtgtagg ctttcatctt
     3661 tgttttagct ccctgccagg gcagcggtgg ttgggcgggt gagtcgccgc cggggtggat
     3721 tgtcgccgct gctctctgta agtgtacccg atgccccatg cccggcaacc aaacccccgt
     3781 ggcgccaggg ctattccaaa gtcaccccag gcgcaccgac ctgacaggtg agcggccacg
     3841 aatcgtactt gcaccagaga acaactggcc gctcacccgt caggtcaatg gtcgctagac
     3901 gactttggaa tagccctgct gtggcgcgat tctccagaat cgcgtccttg cgccccgttg
     3961 tgctagggca tggagggcat ggaaccgccg tcttcggccg gggccggcgt gagttccgcg
     4021 ccccccttgg ccgccagcag ctcgcgcatg tagtcgatct ccagttgctg cgcctcgaca
     4081 atcgacgccg ccatcgtccg caccatcggc tggtcggcgt tttcggccgc cgcggccgcc
     4141 atcgccacgc cgccctgatg gtgggggatc atcagttgca ggaaaagcac gtcggccgct
     4201 tcaccaccgg cggcgcgcaa cgcggccagt tgctcggccg tggccatgcc cggcatcagc
     4261 ccctcggtcg gcatgcccat ccaggccatg cgcggcccgc tactcgtcca cgccagtccc
     4321 caggcatcca gccagccgct catgatgccg atctggccct gctgcgtagt caggatgtcg
     4381 taggccaggg tccgaatagc cgggtcgtcg ctgcgatcat agaccaggcc agccatgacg
     4441 acggcctgct cgtggtggag gctcatgtcg cgggcaaagc cggcatcgac cgagtcggct
     4501 gcgggcacgc ggccgcggcc gagccagtag ccgcccagcg ccccaacggc cagggccgcc
     4561 accgcgacaa ccaacaggag ccagaggcgg ttggggggcg aggccggagc atcagcggag
     4621 aagtcagcgt cagccggccg agaatcgagt gcagtatcca tagccatatt ggtatcagta
     4681 tttcgtgtag tagagataga tcactatcat ttaagccgcc a
//
LOCUS       DAOSMQ010000238         4714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18195_length_4714_cov_4.624168, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000238 DAOSMQ010000000
VERSION     DAOSMQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18195_length_4714_cov_4.624168"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="PK829_08870"
     CDS             <1..1503
                     /locus_tag="PK829_08870"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOU41367.1"
                     /translation="GATVRANTADVGGGLAATQGEALLLNSTIDANTAATDGRGGGLY
                     LLEMTATTLTGSHVTGNHAGEGAGLAVIDGPATLTGGEISGNVATRSGGGLLAPTGTV
                     ALSGVLLADNTAGESGGGIFTDTGRVTLANVTLRANAAASGGGLAALTGTIIIQGGAL
                     TGNTSRGDGAGAATGGPLTAAGATFEGNVAGGDGGGLASSGAATLTNVVLRANRALDG
                     GGVAVSAGTVTIDGGRLANNRATGSGGGAHAGAGRLTIIAATVAGNEAVSGGGLFAAE
                     GRIDVAGATVSGNRAGDGGGLRAALDGTITLVNTTISGNTANVAGGGIHNAGQVTLSN
                     ATVSGNTALTGGGAFNYSGALTLQRTLLAGNHAPTGPEARRLGGAVVVGARNVFGFGG
                     DAGLSGLSPGAGDMVPAVALAAVLSPLAANGGPTQTHALPSFSPAIDRAPSAACAAAP
                     VSGVDQRGQPRNIDGNGIPSANECDIGAFEAPPGSAPGEYRALAPLILRP"
     gene            complement(1528..2625)
                     /locus_tag="PK829_08875"
     CDS             complement(1528..2625)
                     /locus_tag="PK829_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU41368.1"
                     /translation="MAKKRTQINLSQPIQPRGGDLERLFATSGDVEQAAGLQLIAVRL
                     DAIDPDPDQPRSTFHEDALRELADSIRQDGVIQPIEVTQSSPGRYLIVHGERRWRAAQ
                     LAGLETIPAVVRRRDYDEVTRFVRQVVENIQREDLNDVDRAAALLRLRKLMQEEVDAT
                     RRERGADAPKDEPWAGKVTWSKVGGRLGYSRQRIHQLIQLLDLPEEIRDAVRDGTLSE
                     RDTRVYQGLTTIQQRALHRARLARSLDANDIRSVARLLREAPDMTVATAVRLLRQKQD
                     EHATTGVAGAPTPLLPRASADPDAAFGGDPVLPTRVGRPTSIERLDWVRGHLARVERQ
                     GLTASERRELLRLLRLIRDDVASLMAALEEG"
     gene            complement(2685..3467)
                     /locus_tag="PK829_08880"
     CDS             complement(2685..3467)
                     /locus_tag="PK829_08880"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOU41369.1"
                     /translation="MSRIIAVAMQKGGVGKTTTTVNLAAALAEMGQRVLAVDLDPQGN
                     LTQHAGFDPDTITPTLYDALKAEVDGGAGDVRAAIVATRDGFDLLPSQPELSLVEVAL
                     INTLSRERILAGLLDSVAADYDTIFIDCNPSLGLLVINALTAADSVLIPIQTEYLAAR
                     GALMILSTIETIRRKRLNPRLTIEGILLTMADTRASLTRDIQQAVEQQYGGNIRIFST
                     VIRRSVRFAESAAAGRSLIAYDPRSAGANAYRAVARELGNRN"
     gene            complement(3605..4393)
                     /locus_tag="PK829_08885"
     CDS             complement(3605..4393)
                     /locus_tag="PK829_08885"
                     /inference="COORDINATES: protein motif:HMM:NF022022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HOU41370.1"
                     /translation="MSLVVIEQREVEFYGDELIAVRAADGHIYVSVRHLCDALGLDRT
                     AQVRRIRRQEILDEAYKGGAILTPPSKGGVGGGRQQLGLIRVDVVPLFLTGVSVNSVR
                     EEIRPKLARFQREAAKVLWEAFQQGQLTADLDFDALLAQDTPEAQAYRLAQAVLQLAR
                     NQLLMRAQLADHEQRLETIESQLGDTGRNVTPDHIVRMGFLAESLALALGQKTNRNEF
                     GAVYGELYRKFGITGYKMLPAHRFDEAMKFLTDWHENLGGPLPF"
BASE COUNT          718 a         1505 c         1736 g          755 t
ORIGIN      
        1 ggcgcgactg tgcgggccaa cacggccgac gttggtggcg gtctggccgc cacccagggc
       61 gaggcgttgc tgctcaatag caccatcgac gccaacacgg ccgcgaccga cggccggggc
      121 ggcggcctct acctgctcga gatgacggcg acgacgctga ccggcagcca cgtgaccggc
      181 aaccacgccg gcgagggcgc ggggctggcc gtgatcgacg ggccggcgac gctgaccggc
      241 ggcgagatca gcggcaacgt ggccacgagg tcgggcggcg ggctgctggc cccaacgggc
      301 acggtggcgc tgagcggcgt gctgttggcc gataacacgg ccggcgagtc gggcggcggc
      361 atcttcacgg acacgggccg cgtgacgctg gccaacgtga cgctgcgcgc caacgccgcc
      421 gccagcggtg gcggcctggc ggcgctgacg ggcacgatca tcatccaggg cggggcgctg
      481 accggcaaca ccagccgcgg cgacggcgcg ggcgcggcca ccggcgggcc gctgaccgcg
      541 gccggggcaa cctttgaggg caacgtcgcc ggcggcgacg gcggcgggct ggccagcagt
      601 ggcgcggcaa cgctgaccaa cgtcgtcctg cgcgccaacc gcgccctcga cggcggtggt
      661 gtggccgtgt ccgccggaac ggtgaccatc gacggcggac ggctggccaa caaccgcgcc
      721 acgggcagcg gcggcggcgc gcacgcgggc gcgggccggt taaccatcat cgcggcgacc
      781 gttgccggca acgaagctgt cagcgggggc gggttgttcg ccgccgaggg gcgcatcgac
      841 gtggccggcg cgacggtcag cggcaaccgc gccggcgacg gcggcggcct gcgcgcggcg
      901 ctcgacggga cgatcaccct ggtgaacacc accattagcg gcaacacagc caatgtcgcc
      961 ggcggcggca ttcacaacgc cggccaggtg acgctgagca atgccaccgt gagcggcaac
     1021 acggccctga ccggcggtgg ggccttcaac tatagcggcg cgctgacgtt gcagcggacg
     1081 ctgttggccg gcaaccacgc gcccacgggg ccggaggcgc gccggttggg cggcgccgtc
     1141 gttgtggggg cgcggaacgt gtttggcttc ggcggcgacg cggggctgag tgggctgtcg
     1201 ccgggagcgg gcgacatggt gccggccgtg gcgctggccg cggtgctgtc gccattggcg
     1261 gccaacggcg ggccgacgca gacccacgcc ctgccgtcct tcagcccggc catcgaccgg
     1321 gcaccctcgg ccgcctgcgc cgccgcgccg gtcagtggcg tcgatcagcg tgggcagccg
     1381 cgcaacatcg acggcaatgg catcccctcg gccaacgagt gcgacatcgg cgcgttcgag
     1441 gccccgccgg gcagcgcgcc gggcgaatac cgcgccctcg cgccgctgat cctgcggcca
     1501 taagcgccgg ccaagcccag cgggggccta gccctcttcc agcgccgcca tcagcgaggc
     1561 cacgtcgtcg cggatgagac gcaggaggcg cagcaactcg cgccgctcgg aggccgtcag
     1621 cccctgccgc tcgacgcggg ccaggtggcc gcgcacccag tcgaggcgct cgatgctggt
     1681 cgggcggccg acgcgggtgg gcagcaccgg gtcgccgccg aaggcggcgt cggggtcggc
     1741 cgaggcgcgc ggcagcagcg gggtgggggc gccggccacg ccggtggtgg cgtgctcgtc
     1801 ttgcttctgg cgcaacaggc gcacggccgt ggccacggtc atgtccggcg cttcgcgtag
     1861 caatcgggcg acgctgcgga tgtcgttggc gtcgaggctg cgagccaggc gggcgcggtg
     1921 gagggcgcgc tgctggatgg tggtaaggcc ctggtagacg cgcgtgtcgc gctcgctcag
     1981 cgtgccgtcg cgcacggcgt cgcgtatctc ctctggcagg tcgagcaact ggatgagctg
     2041 gtggatgcgc tggcgggagt agcccaggcg gccgccgacc ttcgaccagg tgaccttgcc
     2101 ggcccagggt tcgtccttgg gtgcgtccgc gccgcgttcg cgccgggtgg catcgacctc
     2161 ctcctgcatc agcttgcgca gccgcagcag ggccgccgcc cggtcgacgt cgttcaggtc
     2221 ttcgcgctgg atgttttcga cgacctggcg gacgaaacgg gtcacctcgt cgtagtcgcg
     2281 gcggcggacg acggcgggga tggtctccag gccggccagt tgcgccgcgc gccagcgccg
     2341 ctcgccgtgg acaatcaggt agcggccggg cgaggattgc gtcacctcga tgggttggat
     2401 aacgccgtct tggcggatgc tgtcggccag ttcacgcagg gcgtcctcgt ggaaagtgct
     2461 gcgcggctgg tcggggtcgg ggtcgatggc gtcgaggcgg acggcgatga gttgcagccc
     2521 ggcggcctgc tccacatcgc cgctggtggc gaagaggcgc tccaggtcgc cgccgcgcgg
     2581 ctggatgggc tgggacaggt tgatttgggt gcgctttttg gccatgctca atcaattagg
     2641 aaataggaat taggaactac gaattaggaa tggctcttcg attcctaatt cctatttcct
     2701 aattcccgag cgaccgcccg gtacgcattc gccccggcgc tgcgcgggtc ataggcaatc
     2761 aggctgcggc cggcggcggc gctctcggcg aaacggacgg agcggcggat gacggtgctg
     2821 aagatgcgga tgttgccgcc gtactgctgt tcgacggcct gctggatgtc gcgggtcagc
     2881 gaggcgcgag tgtcggccat cgtcagcaga atgccctcga tggtcagccg cgggttgagc
     2941 cgtttgcggc gaatggtctc gatggtggaa aggatcatca gcgcgccgcg ggcggccaga
     3001 tattcggtct ggatggggat gaggacgctg tcggccgcgg tcagggcgtt gatgaccagc
     3061 aggcccagcg acgggttaca gtcgatgaag atagtgtcgt agtcggcggc aacgctgtcg
     3121 agcagcccgg ccaggatgcg ctcgcggctg agggtgttga tcagggcgac ctcgaccagg
     3181 ctgagttccg gctggctggg cagcaggtca aagccgtcgc gcgtggcgac gatggcggcg
     3241 cgcacgtcgc ccgcgccgcc gtccacctcg gccttcaagg cgtcgtagag cgttggcgtg
     3301 atcgtatcgg ggtcgaagcc agcgtgctgg gtcaggttgc cttgcgggtc gagatcgacc
     3361 gccaggacgc gctgccccat ctccgccagc gccgcggcca ggttgacggt tgtcgtcgtc
     3421 ttgccgaccc cgcccttctg catcgccact gcgataatcc tggacataat aattaggaat
     3481 tagaaattag gaattcagac atggacatgg ttgtgaactg gtgaccgctt gagctggttg
     3541 gtcgatgcga atgtaacatg ttcttctctt gattcctaat tcctgactcg tcattcgtca
     3601 ttgattaaaa gggcagcggc ccgcccaggt tctcgtgcca atcggtcagg aacttcatgg
     3661 cttcgtcgaa gcggtgggcg ggcagcatct tgtagccggt gatgccgaac ttacggtata
     3721 gctcgccgta gaccgcgccg aactcgttgc ggttggtctt ctggcccagc gccagggcga
     3781 gcgattcggc gaggaagccc atgcgcacga tgtggtcggg ggtgacattg cggccggtgt
     3841 cgcccagttg cgactcgatg gtctccaacc gctgctcgtg gtcggccagc tgggcgcgca
     3901 tgagcagctg gttgcgggcc agttggagca cggcctgggc caggcgataa gcctgggcct
     3961 ccggcgtgtc ctgggccagc agggcgtcga agtcgaggtc ggcggtcagt tgtccctgtt
     4021 ggaacgcttc ccatagcacc ttggccgctt cgcgctggaa gcgggccagc ttggggcgaa
     4081 tctcctcgcg gacgctgttg acgctgacac cagtcaggaa caggggcacg acatcgacgc
     4141 ggataaggcc gagttgctgc cggccgccgc caacgccccc tttagaaggg ggtgtcaaaa
     4201 tggcaccccc tttataggcc tcgtccaaaa tctcctgtcg ccggatgcgg cgcacctggg
     4261 ccgtccgatc cagacccagc gcgtcacaca ggtgacggac tgagacgtag atgtggccgt
     4321 cggcggcgcg gacggcgatc agttcatcgc cgtagaactc gacttcgcgt tgttcgatca
     4381 cgacaagact catgtcttcc tctccaacct gcgtgttggc agtggctggc cctggcccta
     4441 ctccaccagg gagtggtcaa agggatcatc tcctggcacg acggctgttt tctttcgcca
     4501 gggcatgcca accatcaccc gctccaggcg acatcgctgc cggcctgttg cgacattgca
     4561 acaattatga cacgtcgttc cgaattcgcc aacagtgggt gaaagggagg atagccagcg
     4621 tacctggccg gtcattgccc gcccacaagg gacaacgaca cggccaggct ataaggggga
     4681 aggcgattcg ggcgaatcgc cctcgttgcc agag
//
LOCUS       DAOSMQ010000239         4711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18204_length_4711_cov_4.685352, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000239 DAOSMQ010000000
VERSION     DAOSMQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18204_length_4711_cov_4.685352"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PK829_08890"
     CDS             complement(<1..416)
                     /locus_tag="PK829_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41371.1"
                     /translation="METRLPLLVVLLPLLAVLPAQLLGRRRRAATAVGLVAVVVLLAL
                     VALAGTLTGDATAVVFGPTLTLAARARGLLALVYVLAGGLLLLDWFRPVGRGFVPLAL
                     AALAPLAAALMAAPAGVALVLLAVGLACWPRPSTAGA"
     gene            complement(443..1180)
                     /locus_tag="PK829_08895"
     CDS             complement(443..1180)
                     /locus_tag="PK829_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41372.1"
                     /translation="MPTIFDILDRLAWLRGAPAVAVVLVAAAVAVGAWDIGLARRGRT
                     VALPFAPLAFPALLVVYLVSGLLLIDVLDPRLAVVYVLMGVVIALIVLLTGAQTRWGQ
                     PPPGLTDAETARLSRPRPRAAGPLVFTSRAVLRLGLALAVLAGALWLARTPDTWLLFL
                     PEGRAYLEVAVFGLAGLGLAGVVASPEPLPAGVGLLLFLLAFALYYGMVDPSITMVVA
                     LVVLQLVVALVAAYLAQARTLPVDLLD"
     gene            complement(1190..2659)
                     /locus_tag="PK829_08900"
     CDS             complement(1190..2659)
                     /locus_tag="PK829_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41373.1"
                     /translation="MLPILLSGALLAAAVVCAVAARRLPRANAPFAPPGRAHLLVALA
                     VLIPLLVWLAWPGARAGDRAAAWQLTGSALWLLAVVVARLALGRAVNGAVAGRLATAF
                     GLTAALLPTLWATDERTRVVAVALLAVGWLAAWWRGAGRAATVWPAAGLLAVVMLLWA
                     ATAVGGAAGVVFVVAAAVLLLGIWPLAGAPWGSDDLAPLLVGLPVLAGAALLLSVARG
                     VALPPGLLAAATAVGLLAIVVGLARLGSQSPGGVARALAPALGGLALVAGVWGGEAAL
                     LPAARLAVFAPAALALLGPAAGRGRWSRLAVVAVVYAALAGLPLTAGFGALSRLYALW
                     LPGGIVLLAITVGALALWLAVIILGARGAGGENPTGQPLMAALLPAGLAAAALVRPDV
                     SGLALPPAVWVAVTLPALIGALLGWFAPGLWHVGDLARESFAASALVGRLGARLGPTA
                     RRAGATAAAALGDAVSILEGDNGLLLLLALAALLLWLGR"
     gene            complement(2745..3593)
                     /locus_tag="PK829_08905"
     CDS             complement(2745..3593)
                     /locus_tag="PK829_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU41374.1"
                     /translation="MSSNTGRLRVGLALGGGVARGLAHIGVIAVLEEAGVPIDCVAGT
                     SMGAIIGASYCAGLTVDELKANAARAGWWDISRPLPTANGLITFNRLERWLEDTIGEY
                     DVRDLAIPFAAVASDLVSGERVVLSRGRLCTAVRASSSVPGFAHPVELDGRLLVDGGI
                     TDNVPADVARLLGADYVIGVDVFAHVLRRRLGPLGFGVAAIETMVRHAGRGDAECDCL
                     IVPPMEGRSYFNFSHHEKFIALGEEAARRQLPAILAALARAERAVPTAVEPLPLAQVD
                     HILIEA"
     gene            complement(3654..4064)
                     /locus_tag="PK829_08910"
     CDS             complement(3654..4064)
                     /locus_tag="PK829_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOU41375.1"
                     /translation="MGIRFATDEWAKALMREVNNSEAYAKAAAHWEGDFYFITSAGPG
                     MPKDTYLYLDLWHGKARDAYLVADPASKPVAFELRAPLDVWRKVMEKKVDPIRGIISG
                     QLKLKGNMMTVLKAPKAATELVACAMKVDTEWPQ"
     gene            complement(4181..>4711)
                     /locus_tag="PK829_08915"
     CDS             complement(4181..>4711)
                     /locus_tag="PK829_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOU41376.1"
                     /translation="TDEFQSVLTRLERLELPTIALLHGHCLGLALELALACDLRLAAE
                     GTLLGLPETLLGMIPDVGGTTRLTRLVGPARAKELIFTGRRIDATTAERWGFVNAVVA
                     PDELSARGEALAAEIATAAPLAVGMAKRVIDGLADIDRGLLLEGWAQSQLFSSEDFME
                     GAQAFMLRRRPDFKGR"
BASE COUNT          869 a         1782 c         1567 g          493 t
ORIGIN      
        1 gcgcccgccg tagagggccg cggccagcac gccagcccca ccgccagcaa caccagcgcc
       61 acgcccgccg gcgcggccat cagcgccgcg gccagcggcg cgagagccgc cagcgccagg
      121 gggacgaagc cgcgccccac cggccggaac cagtccagca gtagcaggcc gccggcgagg
      181 acgtagacca gagccagcaa gccccgcgcc cgcgccgcca gcgtcagcgt cggcccgaag
      241 acaaccgctg tggcgtcgcc ggtcagcgtg ccggccagcg cgaccagggc cagcagcaca
      301 accacagcga ccaggccgac ggccgtggcc gcccgtcgtc gccggccgag caactgcgcg
      361 ggcaaaaccg ccaacagcgg caggaggacg acgaggagag gtaggcgggt ttccatgatg
      421 gtgatgggga gctatggggc ggtcagtcga ggagatcgac gggcagagtg cgcgcctgag
      481 ccaggtaggc agccaccagg gccaccacca gttgcagcac gacgagggct acaaccatcg
      541 tgattgacgg atcgaccatg ccatagtaca gggcaaaagc cagcagaaac agcagcaggc
      601 ccacgcccgc cggcagcggc tccggcgaag ccacgacgcc ggccagtccc aacccggcca
      661 gcccgaaaac ggccacctcc agataggcgc ggccctctgg cagaaagagt agccatgtgt
      721 ccggcgtccg cgccagccac agcgcgccgg ccagcacagc cagcgccagc cccagccgca
      781 gcacagcccg gctggtgaag accaacgggc cggccgcccg cgggcgcggg cggctgagcc
      841 gcgccgtctc ggcgtcggtc aggccgggcg gcggctgccc ccagcgcgtc tgcgctccgg
      901 tcagaaggac gatgagggcg atgaccacac ccatgaggac gtagaccacg gccaggcgtg
      961 ggtcgagcac gtcgatgagt agcaagccac taaccaggta gacgaccaac agggccggga
     1021 aagccaacgg cgcaaacggc agcgccaccg tccggccgcg ccgcgccagg ccgatatccc
     1081 acgcgcccac ggccacggcc gcggccacaa ggacgacggc caccgccggc gcgccgcgca
     1141 gccaggccaa tcgatccaga atgtcgaata tcgtcggcac gtgacttccc taacggccca
     1201 gccagagcaa cagcgccgcc agcgccagca gcaagagcag gccattgtcc ccttcaagga
     1261 tactcaccgc gtcgcccagg gccgcggccg ccgtcgcgcc ggcgcgacga gccgtcgggc
     1321 cgaggcgcgc gcccagccgc ccgaccagcg cgctggcggc gaacgactcc cgcgccaggt
     1381 cgcccacgtg ccacagcccg ggcgcgaacc agcccagcag cgcgccgatc agggccggca
     1441 gcgttacggc cacccagacg gcgggcggca atgccagccc ggatacgtcc ggccgcacaa
     1501 gggccgcggc cgccagcccc gccggcaaca aagccgccat gagtggctgc ccggtggggt
     1561 tctccccccc ggccccgcgc gcgcccagga taatgaccgc cagccacagc gccaacgcgc
     1621 cgacggtgat ggccagcagg acgatgccgc ccggcagcca gagggcgtag agccgcgaca
     1681 gcgccccaaa gccggccgtc agcggcagac cggccagggc cgcgtagacc acggccacga
     1741 cggccagacg cgaccaccgg ccgcggccgg ccgccgggcc aagcagcgcc aacgccgccg
     1801 gcgcaaagac ggccaggcgc gccgcgggca gtaacgccgc ctcgccgccc cagacgccgg
     1861 cgaccagcgc cagcccgccc agagccggag ccagcgcccg ggccacgccg ccgggcgact
     1921 gcgaaccaag gcgagccagg ccaacgacaa tggccagcag gcccacggct gtcgccgcgg
     1981 ccagcagccc cggcggcagc gccacaccgc gggccaccga caacagcagc gccgcgccgg
     2041 ccagcaccgg cagcccgacc agcagcgggg ccaggtcgtc cgacccccac ggcgcgccgg
     2101 ccagcggcca aatgcccagc aagagcaccg ccgcagccac gacgaagaca accccggccg
     2161 cgccccccac ggccgtcgcc gcccagagca acatgaccac ggccagcagc ccggcagccg
     2221 gccagaccgt cgcggctcgg cccgcgcccc gccaccacgc cgccagccag ccaacggcca
     2281 ggagggccac agcgacgacg cgcgtgcgct cgtctgtggc ccacagggtg ggcagcagcg
     2341 ccgccgtcag accgaaggcg gtggccagcc ggccggccac ggccccattg acggcgcggc
     2401 cgagcgccag ccgggcgacg acgaccgcca gcaaccacag ggcgctgccc gtgagttgcc
     2461 aggccgcggc gcggtcgccg gcgcgcgcgc cgggccacgc cagccagaca agtagcggga
     2521 tgagcacagc cagggcaacc agtaggtggg cgcggcccgg aggggcgaag ggcgcattgg
     2581 cccgtggtag acggcgagcg gccacggcgc aaaccacggc cgcggccagc agcgcgccgc
     2641 tcaaaaggat gggcaacatg gacaacaagg acatcgaagc ccggattcta gtctccaccg
     2701 ggcttccggg caaagccggg acgaaaactc tcagccggcc gtccctatgc ctcgatgagg
     2761 atgtggtcga cctgggccaa cggcagcggc tcgacggccg tcggcacggc ccgctcggct
     2821 cgcgccagcg ccgccaggat ggccggcaat tgccggcgcg ccgcttcctc gcccagggca
     2881 atgaacttct cgtgatggga gaagttgaag tacgagcggc cctccatcgg cggcacgatg
     2941 aggcaatcac actcggcgtc gccgcggccg gcatggcgca ccatcgtctc gatggctgcc
     3001 acgccaaagc ccagcgggcc gagacggcgg cgcaacacgt gcgcgaagac atccacgccg
     3061 ataacgtagt ccgcgcccag caaccgggcc acgtcggcgg gcacgttgtc ggtgatgcca
     3121 ccatcgacca gcagccggcc gtccagctca accggatggg cgaagccggg gacggaggag
     3181 ctggcgcgaa cagccgtgca cagccggccg cgcgacaaca ccacgcgctc gccgctcacc
     3241 agatcgctgg cgacggcggc aaaggggata gccaggtcgc gcacgtcata ctcgccgatg
     3301 gtgtcctcca gccagcgctc caaccggttg aacgtgatca gaccattggc cgttggcaag
     3361 gggcgggaaa tatcccacca gccggcgcgg gcggcattgg ccttcagctc atccacggtc
     3421 aggccggcgc agtaggacgc gccaatgatg gcccccatgc ttgtgccggc gacgcagtcg
     3481 atgggcacgc cggcctcttc cagcacggcg atcacgccaa tatgggccag gccgcgggcg
     3541 actccgccgc ccagcgccag gcccacacgc aaacgacccg tgttactgct catggctcgt
     3601 cctcgcacct ttgccagcgg gctactcacc gcgccgcatt tacggctacc cgcctattgg
     3661 ggccactctg tatcgacctt catggcacag gctaccagct ccgtcgccgc cttgggcgct
     3721 ttgagcacgg tcatcatgtt gcctttgagc tttagctggc cggaaatgat cccgcggata
     3781 ggatcgacct tcttttccat gaccttgcgc cagacatcga gcggggcgcg caactcgaac
     3841 gccaccggct tcgacgcggg gtcagccacc aggtaggcat cgcgcgcctt gccgtgccag
     3901 aggtctagat agagataggt gtccttcggc atccccggcc cggccgaggt gatgaagtag
     3961 aaatcccctt cccaatgggc ggcggctttg gcataggcct cgctgttgtt gacctcgcgc
     4021 atcagtgcct tggcccactc atcggtcgcg aacctgattc ccatgtcgtg ctctcccata
     4081 tgatgctagt cgtgaacggc aatcggtgat tcttgtaaag atagcacaca ccctcaagaa
     4141 tcgcacgcgg ataatgttgc ttgcaggaca atcacatttt ctaccgtccc ttgaaatccg
     4201 gccgccgccg cagcatgaac gcctgcgccc cttccataaa gtcctcgctg ctgaagagtt
     4261 gactctgcgc ccagccctcc aacagcagcc cgcggtcgat gtcggccagg ccgtcgatga
     4321 cgcgcttggc catgcccacg gccagcggcg cggcagtggc gatctcggcc gccagggcct
     4381 cgccgcgggc gctcagttcg tccggcgcga ccacggcgtt gacgaagccc cagcgctcgg
     4441 ccgtggtcgc gtcgatgcgc cggccggtga agatcagttc cttggcccgc gccgggccga
     4501 ccaggcgcgt caggcgcgtc gtgccgccca cgtcgggaat catgcccaac agcgtctccg
     4561 gtaggcccag cagcgtcccc tcggcggcca gccgcaggtc gcaggccagg gccaactcca
     4621 gggccaggcc caggcagtgg ccatgcagca aggcgatggt cggcagttcc agccgctcca
     4681 gccgcgtcag cacgctctgg aactcgtcgg t
//
LOCUS       DAOSMQ010000240         4708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18217_length_4708_cov_4.878358, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000240 DAOSMQ010000000
VERSION     DAOSMQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18217_length_4708_cov_4.878358"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2839
                     /gene="dnaE"
                     /locus_tag="PK829_08920"
     CDS             <1..2839
                     /gene="dnaE"
                     /locus_tag="PK829_08920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU41377.1"
                     /translation="LDHDHEFYSADLEQKYKSDPRVRELVDTAKQLEGVARHASSHAA
                     GVIISDKPLVEYVPLHRPTSGESGLGGIRSVSQWPMEIIEKIGLLKVDFLGLSTLTVM
                     RLAAQLIAQRHGVHYTMDNIPYDIGQFGPPPDDDKGMDKAFAMLARGDVQGVFQVEGS
                     GMRKLMMDMKPQRFDHIIAAISLFRPGPMENIPEYIRRMHAMLFEGKDIAVYHAPELK
                     PILEDTYGILVYQEQIIRIAAELAGYEPGEADMIRKAVSKKKRDLMDQHKAQFTAGAM
                     QRGFSQEVCDAIWGDIEFFARYGFNKAHAADYAVICGQTAYLKAHYPVEYITALLTVD
                     HADTEKVAKYVADARRLGITVAPPSLNRAQLDFTIEDSYAEGPGSKGARARGSKSGAA
                     DAFFSPASLRPGSPAANRATIRYGMAAIKNAGEGAVQSIIDEREANGPFRDPVDLAER
                     IDLRRIGKRALESMVKVGVFDEWGTRPQLLDALDRMIGHSGSTHAAAAAGQMTLFGGN
                     GGNFKVAVNLMRSDKDVPGIERRQLLDWEKELIGVYLTEHPLQQRLGDLQSIVTARTG
                     EIDAAWNGKAVTLAGLVVGSRTTNTKKGQAMAFVTLEDLDGKVDLVFFPKVWAACREL
                     VQTNQILVVRGQVQAQNESISILVNSAQTKLTVAQDAATRGLPNWDAPPDWGNDDPWT
                     GSDDMRGYAPAARPAAINGPAANGVIGPAPAAQVGESAPVYGYAARVAGDEDETEDND
                     DYWDDEPAADEEAMTPPDRAAADEVEPPPAPVVEMPPTAPTPTAPPSTALPSTALPST
                     APPAEARQTNGADGYGPMLAARPPVVEPPAGNGMAGPGVVGPGMVGPSTAGPGAAGPA
                     RDAVRAPVHGGLPPPSRLLVVEIRASGNWKETCRQSVKLAERYAGNAGLRLQLAGQDL
                     VMDFPDRRIDCAIELVEALERLAGVGRVYEC"
     gene            3623..4213
                     /locus_tag="PK829_08925"
     CDS             3623..4213
                     /locus_tag="PK829_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41378.1"
                     /translation="MSQHDDAGERALQTRLAYLLDLDISPSLRPEERLLLAVLRQAVV
                     DYFGDDPLERLSAGLYFARSPLYRMTLRQFGLPDHALPEGVDLSAFRGDLPMNADHAL
                     DPLRLETLVRQLSGTQLKVVLTMGLLSLPATTRKISLNCGLGRATVMAALDQLSRQGL
                     VQRNDDAPHAEWSLGDEVRRVLAEVWGDGPHQIDHS"
     gene            complement(4195..>4708)
                     /locus_tag="PK829_08930"
     CDS             complement(4195..>4708)
                     /locus_tag="PK829_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41379.1"
                     /translation="GALFTILSFPPLLPLLVTATENPAAGFPPPDPVEAAFLLRQAGP
                     EAVGGLAVVGGAPAGFVLLAPDDGARLRAARGGRALWGRAWLALMRGRPARAGRLLFG
                     GVRPAARRRGVGQALWVWALAAAAARGWQTLTVGPVWSPPAGESPAAAFLRAQGAAAR
                     QTYQLYEWSI"
BASE COUNT          815 a         1605 c         1592 g          696 t
ORIGIN      
        1 cctcgaccac gaccacgagt tctactcggc cgacctggag cagaagtaca agagcgaccc
       61 gcgcgtgcgc gagctggtgg acacggccaa gcagttggag ggcgtggccc gccacgccag
      121 cagccacgcc gccggcgtca tcatctccga caagccgctg gtggagtatg tgcccctgca
      181 ccggccgacc agcggcgagt ctggcctggg cggcattcgc tccgtcagcc agtggccgat
      241 ggagatcatc gagaagatcg gcctgctgaa ggttgacttt ctgggcctga gcacgctgac
      301 ggttatgcgt ctggcggcgc agctcatcgc ccagcgccac ggcgtccatt acacgatgga
      361 caacatcccc tacgacatcg gccagttcgg cccgccgccg gacgacgaca agggcatgga
      421 caaggccttt gccatgctgg cccgtggcga cgtgcagggc gtcttccagg tggagggatc
      481 ggggatgcgc aagctgatga tggacatgaa gccgcagcgc ttcgaccaca tcatcgccgc
      541 catctccctc ttccggccgg ggccgatgga aaacatcccc gaatacatcc gccgcatgca
      601 cgcgatgctg ttcgagggca aggacatcgc tgtctaccac gcgccagagt tgaagcccat
      661 cctggaggac acctacggca tcctcgtcta ccaggagcag atcattcgca tcgccgccga
      721 gctggccggc tacgagccgg gcgaggcgga catgatccgc aaggccgtct ccaagaagaa
      781 gcgcgacctg atggaccagc acaaggcgca attcaccgcc ggggccatgc agcgtggctt
      841 ctcgcaggag gtatgcgacg ccatctgggg cgacatcgag ttcttcgccc gctatggctt
      901 caacaaggcc cacgccgctg actatgccgt tatctgcggc cagacggcct acctgaaggc
      961 ccactacccg gtcgagtaca tcaccgccct gctgacggtt gaccacgccg acacggagaa
     1021 ggtggctaag tacgtcgccg atgctcgccg gctgggcatc accgtcgccc cgccgagcct
     1081 taaccgcgcc cagctcgatt tcaccattga ggatagctac gcagaagggc cggggagcaa
     1141 gggcgcgagg gcgcggggga gcaagagtgg ggcggccgat gcttttttct ccccggcttc
     1201 cctgcgcccc ggctccccgg ctgccaatag agcaacaatc cgctacggca tggcggccat
     1261 taagaacgcc ggcgaaggcg cggtgcagtc gatcatcgac gagcgcgagg ccaacggccc
     1321 gtttcgcgat ccggtcgatc tggccgagcg catcgacctg cggcgcattg gcaagcgggc
     1381 attggagtcg atggtcaaag tgggcgtgtt cgacgagtgg ggcacgcggc cgcaactgct
     1441 cgacgctctc gaccgtatga tcggtcacag cggcagcacc catgccgccg ccgctgccgg
     1501 ccagatgacc ctctttggcg gtaacggcgg caatttcaag gtcgctgtta acttgatgcg
     1561 gtctgacaag gacgtgcccg ggatcgagcg gcggcagctg ctcgactggg agaaggaact
     1621 catcggcgtt tatctgaccg aacacccgtt gcagcagcgg ctgggcgacc tgcaaagcat
     1681 cgttacggcg cggacgggcg agatcgacgc tgcctggaat ggcaaggcgg tgacgctggc
     1741 cggcctggtc gtcggctcgc gcacaaccaa cacgaagaag ggccaggcga tggcttttgt
     1801 cactctggaa gacctggacg gcaaagtcga tctcgtcttt ttccccaagg tctgggccgc
     1861 ctgccgcgag ttggtgcaga caaaccaaat cctcgttgtc cgcggccagg tgcaggccca
     1921 gaacgagtct atctctatcc tggtcaacag cgcccaaacg aagctgaccg tggcccagga
     1981 cgccgcgacg cgggggctgc ccaactggga cgcgccgccc gactggggca acgacgaccc
     2041 gtggacaggc agcgatgata tgaggggcta tgcgcccgcg gcccggccgg cagccatcaa
     2101 cggcccagcc gccaacggcg tgattggccc cgccccggcc gcccaggttg gcgagtctgc
     2161 ccccgtctat ggctacgctg cccgtgtcgc cggcgacgag gacgagaccg aggacaacga
     2221 cgactactgg gacgacgagc cggccgccga cgaagaagcc atgaccccgc ccgaccgcgc
     2281 cgccgccgat gaggtcgagc cgccgcccgc gccagtggtg gagatgccgc ccacggcccc
     2341 gacgcccacg gccccgccgt ccacggccct gccgtccacg gccctgccgt ccacggcccc
     2401 gccggcagaa gccagacaga cgaacggcgc ggacggctat ggccccatgc tggcggcgcg
     2461 cccgcccgtg gtcgagccgc cggccggcaa tggcatggcc gggccaggag tggtcgggcc
     2521 aggaatggtc gggccgagta cggccgggcc gggtgcggcc gggcctgcca gagacgcagt
     2581 gcgcgccccg gttcatggcg ggctgccgcc gccgtcgcgc ctgctggtcg tggagatcag
     2641 ggcgtcgggc aactggaagg aaacctgccg ccagtcagtg aagctggccg agcgctacgc
     2701 gggcaatgcc gggctgcggc tgcaactggc cgggcaagac ctggtgatgg actttcccga
     2761 ccggcgcatc gactgcgcca tcgagctggt ggaggcactg gagcggctgg caggagtggg
     2821 acgggtgtat gagtgttgaa gccagccggg gtgggccgag gttcgccccg cgttgagtgc
     2881 ctttctgccg tcaagggcag gctgaacgcc tgcccttttt gttgccggcc gcccggaatt
     2941 cacaccgcgg ggcgtgtctg gcgagcgggt ggcaaaattc acgctttctt cacgcccggc
     3001 cccagccgcc atgatgtcat atttcccggc cagccgcgcc aattttcacg ccctcttcac
     3061 gtcgttgcat gctacactac cgagccaggg acagaacctt tgttctatta ggaagattgg
     3121 agaaggccgc agagggcgac ttgtgtgaca gcggacgacg accggaagcc gtggctttgg
     3181 tatcctcatg ctcaccgcgc tgcggtgagg cctggccgcg ccaccggcgg ccgaagcgct
     3241 cgcggtgatg ccggcgcgcc tcatcttccc catttagctg accaggctat gccgggtggg
     3301 cctgcgtgct ccgccaggga tggaaacgcg cgggccctgg ccggttggga tgcagcgcaa
     3361 cgggcactgc cctacgaagc ttcgctgctg ccggatgaat agccgtcata gggcactgcc
     3421 gggccatacc cgggcaactg cttgaaccag cggaggggag acacagcgcg ggcgcgatca
     3481 acaacgtgtt ggtggactgg gtggaggtgt cgaaaatcga atagcacaaa cgttctattg
     3541 gttgctacac tgggcgcgtc actttcggcc gggcgctctg gctgcccggc gatggaaacc
     3601 gatgaccgct aacaggagca ccatgagcca acacgacgac gccggcgagc gcgccttgca
     3661 aacgcgactg gcctatttgc ttgacctcga catctcccct agcctgcggc cggaggagcg
     3721 gctcttgctg gccgtcctgc gccaggctgt cgtcgattac ttcggcgacg acccgctgga
     3781 gcggctcagc gcggggctgt acttcgcccg ctcgcccctg taccggatga cgttgcgcca
     3841 gtttggcttg cccgaccacg cgctgccgga gggagttgac ctgagcgcct tcaggggaga
     3901 tctgcccatg aatgccgatc atgccctgga cccgctgcgg ctggagacgc tggtgcgcca
     3961 gctatcgggc acgcaactca aggtcgtcct gacgatgggc ctgctgtcgc tgccggcaac
     4021 gacgcgcaag atcagcctca actgtggcct gggccgggcg accgtcatgg cggcgctcga
     4081 ccaactgtcg cggcaagggc tggtgcagcg caacgacgat gccccgcacg ccgaatggtc
     4141 gttgggcgac gaggtgcggc gggtgttggc cgaggtctgg ggcgacgggc cacatcagat
     4201 cgaccactcg tagagctggt aggtctgccg cgccgcggct ccctgggcgc gcaggaaagc
     4261 cgccgcgggc gattctccag ccggcggcga ccagaccggc cccaccgtca gcgtctgcca
     4321 gccacgcgcc gcggccgcgg ccagcgccca gacccacagc gcctgcccga caccgcgccg
     4381 ccgcgcggcc ggccgcactc cgccaaaaag cagccgcccg gcgcgcgccg gccgccccct
     4441 catcagcgcc agccaggccc gcccccacag cgcccgcccg ccccgtgccg cccgcaggcg
     4501 cgccccgtcg tccggggcca gcaggacgaa gccggccggc gcgccaccca ccactgccag
     4561 cccgcccacg gcctccggcc cagcttgccg cagcaggaag gctgcctcaa ccgggtcggg
     4621 tggcgggaaa ccggcggccg ggttttctgt cgccgtgacg agcagcggca gcagcggagg
     4681 aaaagagagg atggtgaaca gcgcgccc
//
LOCUS       DAOSMQ010000241         4691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18296_length_4691_cov_5.593399, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000241 DAOSMQ010000000
VERSION     DAOSMQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18296_length_4691_cov_5.593399"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PK829_08935"
     CDS             complement(<1..1390)
                     /locus_tag="PK829_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOU41380.1"
                     /translation="MTDSNLGDLDPEPETAPIAYDAETQVYDAEPPVQNYSADDFATP
                     PPRNWTGILLAALIVTAVLLGALLAYLFFRDDNNVLIPTTPQTPIPGDNSWATVQTAG
                     RLIVGTSLDYPPFSYRNQDFQPDGFDMALIRDIAGRLRVQTDIRDVAFDSLFTALQQR
                     QIDVAIAAISVTTEREALVGFSNVYYIGEEGILARADSAITAITTLSDMAGRRIGVQR
                     GSVYETWLQRDLVNSGLTVAGNLFVYETVDAAIRDLREQRLDLAIVDQHVAQAAIAQG
                     GVRRVGGGLNKQRYAIAVRRDANALRDQLNLALTQMQNDGRLSQLAQTYLNLDPNAVI
                     TLPAPTATSIVPPTPQPTAPPVGCVNGMRFVADLNLADNNMQSPPPISPGQAFSKGWR
                     IQNSGTCAWTTAYRLAFVSGNTPAASMGGQPTAVQGFVPAGQQYDIFVNLVAPLAPGV
                     YQGIWQMLDDQNR"
     gene            complement(1451..4201)
                     /locus_tag="PK829_08940"
     CDS             complement(1451..4201)
                     /locus_tag="PK829_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41381.1"
                     /translation="MAKKKQLTPAAAFRAVVRAALDHYHEPDWLVANSPLAEAYFLGD
                     RLAADPTDDEARGQALRALLRDAWARLWPGALPADRAALWAATDEARRREGNSGAAYA
                     FLLLELRFFRAYFPPTLYPIQTTDIPVFLSVSTTRFFVHLDEAIDRLADLVLHLAQPT
                     LRPERPRPPAALVGRAAVLAASRGHLLAGRSVTLTGVAGVGKTALAAAIYAGWPTPAT
                     FWYTFLPGLSDNLAGLLFALGHFIRQWHPSALWQQLMADQGQVRNPAQLLAILRADLE
                     RLQPHQPLLCFDEVDMLRTSDGEVHSANHIQLLTFLEGLSTTTAVLLVGQNGYVDTAA
                     HFALNTLDDEQTAALLRDAGIAPQVATVARVQGVTGGLPRLIELVITLIHSGDNLSEL
                     DRLHMRADVRPLFSRLWRRLDGGEQELLTSLAVLRRPVPVDVVARVPAFQPLRHRRIV
                     QVVSGEVAVQPYFRGLIYEALPPEQREAAHAEAARLRAVRGDYTAAAHHYSQAGDVVA
                     AVEVWFPHRRLEIQRGQSGAAQAIFSPISPARLPAPASKQLRLIQNQLDLLEGNAARV
                     VKGMAQVKWETDDIYAAEAHRQAGEANFMLGRTDTALESYGRALIALGHYADAATDAF
                     SRRGQLYIDLGEMAAARAEALRARARLLLLEGMLAFNEGNYDRAWREFGEALEASASL
                     ETPELTAQANEWLAATAIMAGDVATAEHHGTAALEYYRRIGNRVKLEGIRADLAGMYL
                     NVHAYEQVIEPSLRALAYFEEIGHERWISSIASNLAEAYVELGQLDEALRFAQRVLQL
                     ENQRSRPYALYSLGLIHQRQGRPDYAAIAFEDGLRVAQQNGDLYIEAFLRRNVGRLLS
                     KTGRAGEAVAALNAALHLFQRMNLAIEIEATAGELQALSAQTNEEREASSPNLPIAKS
                     PG"
     gene            4436..>4691
                     /locus_tag="PK829_08945"
     CDS             4436..>4691
                     /locus_tag="PK829_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41382.1"
                     /translation="MDPTRPETISFWSLDLRVRDPHPEAGDIRVTDANVNDLITLAYM
                     RWEWQAPRLYDLLPSRSGAPVVPVLGRVEAPTPIPLSLIGD"
BASE COUNT          752 a         1476 c         1685 g          778 t
ORIGIN      
        1 gccggttctg gtcgtcgagc atctgccaga tgccctggta gacgccgggg gccagtggag
       61 ccacgaggtt gacaaatatg tcgtattgct gcccggcggg cacaaagccc tgtacggccg
      121 tcggttggcc gcccatgctg gccgcgggcg tgttgccgct gacgaaggcg aggcgatagg
      181 ccgttgtcca ggcgcacgtg ccgctgttct ggatgcgcca gcccttgcta aaggcctggc
      241 cagggctgat cggcggcggc gactgcatgt tgttgtcggc caggttcaga tcggccacaa
      301 agcgcatgcc gttaacgcag ccgaccggcg gcgcagtggg ctgcggcgtg ggcgggacga
      361 tcgatgttgc cgtgggcgcg ggcagggtaa tcaccgcgtt cgggtcgaga ttgaggtagg
      421 tctgcgccag ctgcgacagg cgaccgtcgt tctgcatctg cgtcagggcc aaattgagtt
      481 gatcgcgcaa ggcgttggcg tcgcgacgca cggcgatggc atagcgctgt ttgttcagcc
      541 cgccgcccac gcgccgcacg cccccctggg caatggccgc ctgggccacg tgctgatcga
      601 caatggccag gtccagccgc tgctcgcgca ggtcgcggat agcggcatcg accgtctcat
      661 agacaaacag attgccggcc acggtcaggc cactgttgac caggtcgcgt tgcagccacg
      721 tctcgtagac gctgccgcgc tggacgccaa tgcgccgccc ggccatatcc gacagggtgg
      781 tgatggcggt gatggctgaa tcggcgcgcg ccagaatgcc ctcctcgcca atgtagtaga
      841 cattgctgaa gccaacgagc gcctcgcgct ccgtcgttac cgagatggcg gcgatggcta
      901 cgtcgatctg gcgctgctgc agggccgtga acagactgtc gaaggcgaca tcgcggatgt
      961 cggtctgcac gcgcaggcgg ccggcgatgt cgcgaatgag ggccatgtcg aagccatcgg
     1021 gctggaagtc ctggttgcga tagctgaagg gtgggtaatc gagtgacgtg ccgacgatca
     1081 gtcgcccggc cgtctgcaca gtcgcccagg aattgtcgcc cggtatgggc gtctgcggcg
     1141 tggttgggat cagaacgtta ttgtcgtccc tgaagaagag ataggccagc aaagccccca
     1201 gcagcacagc ggtaacgatg agcgcggcca ggaggatgcc cgtccagttg cgcggcggcg
     1261 gggtggcgaa gtcgtcggcg ctgtagttct gcaccggcgg ttcggcatcg tagacctgtg
     1321 tctccgcgtc ataggcgatg ggagcggtct ccggttcggg atcgagatca cctaagttgc
     1381 tgtccgtcat gctgctctcc ttgccgcacg tgctgtcact ggcacactgc ccctctgaga
     1441 gtattttatg tcatccgggc gacttggcaa tagggaggtt gggcgatgat gcctcgcgct
     1501 cttcgttggt ctgcgccgat agcgcctgta actcgccggc cgtcgcctca atttcgatgg
     1561 ccaggttcat gcgctggaag agatggaggg ccgcgttcaa ggcggccacg gcctcgccgg
     1621 cgcggcctgt cttgcttagc agccggccca cgttgcgtcg caggaaggct tcaatataga
     1681 ggtcgccgtt ttgctgggcc acgcgcaggc cgtcctcaaa ggctatggcg gcataatccg
     1741 gccgcccctg ccgctggtgg atcagcccca ggctatacag ggcatagggg cgcgagcgct
     1801 ggttctccaa ttgcagcacg cgctgggcaa aacgcagcgc ctcgtccagc tggcccaact
     1861 cgacataggc ctcggccagg ttgctggcga tggaggagat ccagcgctca tggccgatct
     1921 cctcgaagta ggccagcgcc cgcaggctcg gctcgatgac ctgctcgtag gcgtggacgt
     1981 tcaggtacat gcccgccagg tcggcgcgga tgccctctag cttgacgcgg ttgccgatgc
     2041 gccggtagta ctccagcgcc gccgtgccat ggtgctcggc cgtggccacg tcgccagcca
     2101 taatggccgt cgccgccagc cattcattgg cctgtgccgt caattcgggg gtttccagcg
     2161 acgcggaagc ctcgagcgcc tcgccgaact cgcgccacgc ccggtcgtag ttgccctcgt
     2221 tgaaggccag catgccctcc agcagcagca ggcgcgcgcg cgcccgcagc gcctcggcgc
     2281 gcgcggcggc catctcgccc agatcgatgt agagttggcc gcggcgcgag aaggcgtcgg
     2341 tggccgcgtc ggcataatgc cccagggcga tgagcgcccg gccatagctc tccaacgccg
     2401 tgtccgtgcg gccgagcatg aagttggctt cgcccgcctg gcggtgggcc tcggcggcgt
     2461 agatgtcgtc cgtctcccat ttaacctggg ccatgccctt gaccacgcgg gcggcgttgc
     2521 cctccagcag atcgagctgg ttctggatga ggcgaagctg cttggaggcg ggcgcgggca
     2581 gccgggcggg cgagatgggg ctgaagatgg cctgcgccgc gccgctctgg ccacgctgaa
     2641 tctccagccg ccggtgggga aaccagacct cgaccgcggc cacgacgtcg ccggcctggc
     2701 tgtagtggtg ggcggcggcg gtgtagtcgc cgcgcacggc gcgcagtcgg gcggcctcgg
     2761 cgtgggcggc ttcgcgctgc tccggcggca gcgcctcgta gatcaggccg cgaaagtagg
     2821 gctgcacggc cacctcgccg gaaacgacct gcacgatacg gcggtggcgc agcggctgga
     2881 aggctgggac gcgggcaacg acgtcaaccg gcacgggccg gcgcaggacg gccagcgagg
     2941 tcagtagttc ctgctcgccc ccatccaatc gccgccacag ccgtgagaac agggggcgca
     3001 cgtcggcgcg catgtgcagc cggtcgagtt cggacagatt gtcgccgctg tggatgagag
     3061 tgatgaccag ttcgatcagc cgcggcaggc cgccggtgac gccctgcacg cgggcgacgg
     3121 tcgctacctg gggggcgatg ccggcgtcgc gcagcagcgc cgccgtctgc tcgtcgtcga
     3181 gtgtgttcag ggcgaagtgg gcggccgtgt ccacgtagcc gttctggccg accaagagga
     3241 cggccgtggt cgtgctcaac ccctccagga aggtcagtag ttggatgtga ttggccgaat
     3301 ggacttcgcc gtcggaggtg cgcagcatgt ccacttcgtc gaagcacagc aatggctggt
     3361 ggggttgcaa gcgctccagg tcggcgcgca ggatggccag cagttgggcg gggttgcgca
     3421 cctggccctg gtcggccatg agttgctgcc agagggcgga gggatgccat tggcggatga
     3481 agtggcccag ggcgaagagc aggccggcca ggttgtcgct gaggccgggc aggaaggtat
     3541 accagaaggt cgccggcgtg ggccagccgg catagatggc ggcggccagg gcggtcttgc
     3601 cgacgccggc gacgccggtc agggtgacgc tgcggccggc cagaagatgg ccgcggctgg
     3661 cggccaacac cgccgcccgg ccgaccagcg ccgccggcgg gcgcggacgc tccgggcgca
     3721 acgtcggctg ggccaaatgc agcacgagat cggccagccg gtcgatagcc tcgtctagat
     3781 ggacgaagaa gcgcgtcgtg ctgacgctta gaaagacggg gatgtcggtc gtctggatgg
     3841 gatagagcgt cggcggaaag taggcgcgga agaagcgcag ttccagcagc aggaaagcgt
     3901 aggccgcgcc gctattgccc tcgcggcggc gggcctcgtc ggtcgcggcc cacagcgcgg
     3961 cgcggtcggc cggcagcgcc cccggccaca ggcgcgccca ggcgtcgcgc aagagcgccc
     4021 gcagagcctg gccgcgggcc tcgtcgtccg ttgggtcggc cgccagccga tcacccagga
     4081 agtaggcttc agccaacggc gagttagcga ccagccagtc cggctcgtgg tagtggtcca
     4141 gggcagcgcg aaccaccgcc cgaaaggcgg ccgccggcgt cagttgcttc ttcttggcca
     4201 tcgattgtcg aacaacagcg attaacgtcc caatgcccgg tgaggcagcg caagtataac
     4261 caagctgaaa ggggagtcca aaagcgggaa tcgtcgggcc gaattcggga tttctctggg
     4321 tgacgtccgg ccgcggaggg cattataatg gttctgcggc gcagtggggc cggtggctac
     4381 cggtcacgcg tccgccggcc tgttcgcgtt tgattcattt gaaggaggca atcgcatgga
     4441 cccaacccga ccggaaacta tcagcttttg gtcactcgac ctgcgcgtgc gcgatccgca
     4501 cccggaggcg ggcgacatcc gcgtgaccga cgccaacgtc aatgacctga ttaccctggc
     4561 ctacatgcgc tgggagtggc aagcgccgcg gctctacgat cttttgccca gccgcagcgg
     4621 cgccccggtc gtgcccgtcc tggggcgggt cgaagcgccg acgcccatcc ccctgtcgct
     4681 gattggcgac a
//
LOCUS       DAOSMQ010000242         4684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18327_length_4684_cov_5.089868, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000242 DAOSMQ010000000
VERSION     DAOSMQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18327_length_4684_cov_5.089868"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PK829_08950"
     CDS             complement(<1..820)
                     /locus_tag="PK829_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU41383.1"
                     /translation="MLYSDLEDILVKVRASLEHDDVAGAVAALEAMRAPDQADLFHEL
                     EDEHQTALLPQLNPEDAADILEELEDEDAADLVATLPRETLARIIDEMEPDEAADLLG
                     ELEPADQEFVLRQLEDQAEVRPLLLHAEDSAGGLMTSDFLALGRRMSVDEALAALRTW
                     KPDAEDIYDVYVVDRQGRPTGTISMRQLLMEAPQAILADVVETDFVTVPVGSDQEEAA
                     RLMSRYDLATLPVVDTHGRLVGVITIDDVVDVLEEETTEDFQRFGGTQPLDRPYL"
     gene            complement(1153..1539)
                     /locus_tag="PK829_08955"
     CDS             complement(1153..1539)
                     /locus_tag="PK829_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41384.1"
                     /translation="MKQRPLAVTILGVIALVAGILVLLEALLFFGVGFAGLLRLSFFS
                     VAWLGGILSLIVALIWLSTARQLFVLDPRGWMFVVVIAIITLVINVIGLLGGNEFGSL
                     WWSFIAPVLALILAFLPGTRAAFGQK"
     gene            1809..2618
                     /locus_tag="PK829_08960"
     CDS             1809..2618
                     /locus_tag="PK829_08960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41385.1"
                     /translation="MSVDDLLQYDPQSTYGAIALDYDYAQQNYGGFAANHALELAALA
                     PGMTVLDAGCGTGTLTLQAAERVRPGGRVVAVDFVPEMLALASEKASRRALLNIDWRL
                     EDLTAIDLPVASLDVVFCLSTLFAITDMAALTATLWALLRPGGQLIVATPGRPYLAPL
                     HDAFYDAVRAEAPHLTPPRPWQRIDSPERLGAVFKEAGVVATVSEQMLAVPLREPADW
                     WRIVQGTGLSRTARLLGAEAAARVEAANLRAIAERGIDTLTSRFLFAVAAQ"
     gene            2928..3413
                     /locus_tag="PK829_08965"
     CDS             2928..3413
                     /locus_tag="PK829_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOU41386.1"
                     /translation="MRKILEACPTCGSEMIISELTCTTCDTVVRSRYSPCPFCHLAPE
                     DLAFMLLFVRNRGNVKDMERELGVSYWTIRGRLNEIITAMGLDGGAADSDAAAEGETG
                     EATSVPTPPGKVSASAGKEPGRAAAAPASGRPSKAQAILARLQRGEISAQEAAELLAG
                     D"
     gene            3466..4482
                     /locus_tag="PK829_08970"
     CDS             3466..4482
                     /locus_tag="PK829_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41387.1"
                     /translation="MSEEQKQILEMLAGGKINVGEAQLLLAALERGTAPAPDDLKAAA
                     GGPAASGPAAHEERQPGWSPFGPLLEGLGIPRPPRPPAPRAPRPPRPPRPPSRGRRAD
                     VIDQVIQLRAHGIDAAYLNEMRAILGDIDLDDVVQLGIHGVRPEYVREVRGMGLELDT
                     DDLVQFAIHGVRADYVVAMREMGFDLDADDIVQFAIHGVSAKTVARLRDAGLTDLDAE
                     DIVALHIHGIDADFIAMMRAAGFEDLTADDLIGLRIHDVTPEYIERLRAAGLDDLSAE
                     ALTALRIHGVDPAFIERMRGLGLQELDADNLVALKVHGVDADFFAELAEMGLIAPKEK
                     SPEA"
BASE COUNT          802 a         1609 c         1499 g          774 t
ORIGIN      
        1 ccagataggg ccggtcgagg ggctgcgtac caccgaagcg ctggaagtcc tcggttgttt
       61 cctcttccag gacatcaacc acgtcgtcga tggtgatgac gccgacgagc cggccgtggg
      121 tatcgacgac gggcagtgtg gccaggtcat aacgcgacat gagccgggcg gcctcttcct
      181 ggtcgctgcc cacgggcacg gtcacgaagt cggtctcgac gacgtcggct agaatggctt
      241 gcggggcttc catcagcagc tggcgcatgc tgatcgtccc ggtcgggcgg ccctggcgat
      301 cgacgacata gacgtcatag atgtcctcgg cgtcgggctt ccacgtgcgc agcgcggcaa
      361 gcgcttcgtc cacggacatg cgccggccga gggccaggaa gtcggaggtc atcaggccgc
      421 cagcgctgtc ctcggcgtgc agcagcagcg gccgcacctc ggcctggtct tccaactggc
      481 gcaacacgaa ctcctggtcg gctggctcca gctcgcccaa caggtcggca gcctcgtctg
      541 gctccatctc gtcgatgatg cgggccagcg tctcgcgcgg caacgtggcc acgaggtcgg
      601 cggcgtcctc gtcttcgagt tcttccagga tgtcggccgc gtcttccggg ttcagttggg
      661 gcaacagcgc cgtctggtgc tcgtcttcca gctcatggaa caggtcggcc tggtcaggcg
      721 cgcgcatggc ctccaacgcg gctacggctc cggccacgtc gtcgtgctcc agcgacgcgc
      781 ggactttcac cagaatatct tccaggtctg agtacagcat gggacacctc caaagaagcc
      841 tggctccctg gaggcgtggg tggggcgggg aaagggcgac tgccaaccag gggctagtta
      901 ttcggttgac aggggcgggg tctgtctatg actcccgcca ggttctgctt tttcgttcac
      961 ggatgactcc atagtgggtg aaacgcactc tcaccgcgct tccgatgctg cggacgccaa
     1021 cggcaaccgc gtcgcccaac ccgcgtcgag ccgggcgcac tgtcaaagtt tactccccag
     1081 aggtcatttg tcaaggactg ggagacctga caggtgggcc acccagccgc ccacctgtca
     1141 ggtctcagct aactatttct gcccaaacgc cgcgcgcgtc cctggcaaaa aggccagaat
     1201 cagggccaac accggggcga tgaacgacca ccacagcgag ccaaattcgt tgccacccag
     1261 caggccaatg acgttgatca ccagtgtgat gatggcgatg acgacgacga acatccagcc
     1321 gcgcgggtcg agcacaaaca gctgccgcgc agtcgataac cagatcaggg cgacgatgag
     1381 cgacaggatg ccgcccagcc aggccacgct gaaaaagctc aggcgcagca ggccggcaaa
     1441 gccgacgccg aagaatagca gcgcctccag tagcaccaga ataccggcga caagggcaat
     1501 gacgcctaga atggtaacag ccaacggacg ttgtttcatg atgtattctc catagtccac
     1561 gttgatattc gccggcggcg tgattgccgg ccggcgcata tcatcatagc ataaagacaa
     1621 cagtatgcat gaactgcacg gggtttttgt cccgtcgtgg ccggcttttt gacgggttga
     1681 cgcccggtta ccgcttttct ctgagcctaa gcttttgggg gtggccgtta gcgtgctaga
     1741 atcgctctct aaattccatc cgcatcgctg cctacacgtc cgcccccgaa cgataagcga
     1801 gaaaatctgt gtccgtcgat gacctgttgc agtacgaccc gcagtcgacc tacggagcca
     1861 ttgccctcga ttatgactac gcgcaacaaa actatggggg cttcgccgct aaccacgctc
     1921 tggaattggc cgcccttgcg ccggggatga ccgtgctcga tgccggctgc ggcacgggga
     1981 cgctcacgct acaagcggct gagcgggtgc gccccggcgg ccgtgtggtc gccgtggatt
     2041 tcgtcccgga gatgctggct ctggcgtcgg aaaaggccag ccgtcgcgcg ctgctcaaca
     2101 ttgattggcg gctggaagac ctgaccgcca tcgacctgcc tgtggcgtcg ctcgatgtgg
     2161 tcttctgcct atcgaccctg ttcgccatca ccgatatggc cgcgctgacg gcgaccttgt
     2221 gggcgctgct gcggcccggc ggccagctca tcgtcgccac gccgggccgg ccctacctgg
     2281 ccccactcca cgacgctttc tacgacgctg tccgcgccga agcgccccac ctgactccgc
     2341 cgcgcccctg gcagcgcatc gactcgccgg agcggctggg ggctgtcttc aaggaggccg
     2401 gcgtggtcgc cacggtcagc gagcagatgt tggctgtgcc cctgcgcgag ccggccgact
     2461 ggtggcgcat cgtccagggc accggcctca gccgcaccgc ccggctgctg ggcgcagagg
     2521 cggccgcccg cgtcgaggcg gctaatctgc gggccatcgc cgagcgcggc atcgacacgc
     2581 tgacctcgcg ctttctcttc gccgtcgccg cccagtaaac gcttcctccg cccgcaagga
     2641 atggccctcg ccctggcggc gcgcccgccc gcgcggctgc ccacctggcc gcgtcgcctt
     2701 caagctcctt gccccgcgta ccacccacgc cccgcgtcca gatagctgtc cggtgctggc
     2761 ccggtcgagc gccgcgccgt cagagtggcc cctggcggtg gtagagtgca gcccgcccac
     2821 cggccgggca caacaatatt tgtgttcctg cgcccgacga caaaatactt gttgacatcg
     2881 gcaaaatatt ttgctattct gtcaacattc ttgaggagaa ccagcgtatg cgcaaaattc
     2941 tggaagcctg ccccacctgc ggcagcgaga tgatcatcag cgaactaacc tgcacaacgt
     3001 gtgatacggt tgtgcgcagc cgctatagcc cctgcccctt ctgccacctg gcccccgaag
     3061 acctggcttt catgctgctc ttcgtgcgca accgcggcaa cgtcaaggac atggagcgcg
     3121 aactgggcgt gtcctactgg accattcgcg gccgtctcaa cgagatcatc accgcgatgg
     3181 gcctggacgg cggcgcagcg gacagtgacg ccgccgctga gggcgagacg ggcgaggcga
     3241 cgagcgtgcc cacgcccccc ggcaaggttt cggcttccgc cggcaaggag ccggggcgcg
     3301 cggccgccgc ccccgcgtct ggccggccct caaaggcgca ggccatcctg gctcgcttgc
     3361 agcgcggtga aatcagcgcg caggaagcgg ccgagctgtt ggccggcgat taggggcgcg
     3421 cacaggtgtg ccccaacctg acaccatacc gaaaggagac agatcatgag cgaagaacag
     3481 aagcagattc tggagatgtt ggccggcggc aagatcaacg tcggcgaagc gcagctgttg
     3541 ctggccgcgc tggagcgcgg cacggccccg gcgccggacg acctgaaggc cgccgccggt
     3601 ggccccgccg cgagcggccc ggccgcccac gaagaacgcc agcccggctg gtcgccgttc
     3661 gggcctttgc tggaggggct gggcatcccc cggccgccgc ggccgcccgc cccccgcgcc
     3721 ccccggccgc cgcgcccgcc gcgcccgccc agccgcggcc gccgcgccga cgtcatcgac
     3781 caggtgatcc agttgcgcgc ccacggcatc gacgccgcct acctgaacga gatgcgcgcc
     3841 atcctgggcg acattgacct tgatgatgtt gtccaactgg gcatccacgg tgtacggccg
     3901 gagtacgttc gcgaggtgcg cgggatgggc cttgagctgg acactgatga cctcgtccag
     3961 ttcgccatcc acggcgtcag agcggactac gttgtggcga tgcgcgagat gggcttcgac
     4021 ctggacgccg acgacatcgt ccagttcgcc atccacggcg tcagcgccaa gacagtggcc
     4081 cggctgcgcg acgccggcct gaccgacctg gacgccgagg acatcgtcgc cctgcacatc
     4141 cacggcatcg atgccgactt catcgccatg atgcgcgccg ccggctttga ggacctcacc
     4201 gccgacgacc tgatcgggct gcgcatccac gacgtgacgc cggagtatat cgagcggctg
     4261 cgcgccgccg gcctggatga cctctcggcc gaagcgctga cggcgctgcg catccacggc
     4321 gttgacccgg ccttcatcga gcggatgcga gggctggggc tgcaagagct ggacgccgac
     4381 aacctggtgg ccctcaaagt tcacggcgtc gatgccgatt tcttcgccga actggccgag
     4441 atggggctga tcgcgccgaa ggagaagtcg ccggaagcgt agaggccacc ggggagcagg
     4501 ggaagcggac gctaggccct gctcccctgc tccccggccg gcctacggca gcgcgccgac
     4561 cacgtcgatg ccgtccacga cctgcatcag gccgtggtct ttggccttcc acagcggccc
     4621 actgaacgtg ccgccgctcc agctgaacag ctccgacggc ttgaccagcc caccgtcgcc
     4681 ggtg
//
LOCUS       DAOSMQ010000243         4670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18404_length_4670_cov_3.620152, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000243 DAOSMQ010000000
VERSION     DAOSMQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18404_length_4670_cov_3.620152"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PK829_08975"
     CDS             complement(<1..1154)
                     /locus_tag="PK829_08975"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HOU41388.1"
                     /translation="MQAISPRPPRAARARNLGYFLIPPIVGLTLLAVLLALGLTAYRA
                     RYSGRVYTGVSVAGIDLSEKTTAEAEAALSGALPYAGAATVTLVDPGTGTEWSFTPAQ
                     LGIAVDTAATASAAFDVGRSGGPFRRLQDAFQSWYYGRGIAPIVVFNEAQLDRVLAGL
                     SETVERNAVSASWQPNDSGAPAYTPAQIGRSIDRAYLREQLLQPIRAGADARVELLVH
                     DIYPPVYDDTATTEQLAQLYSEPVSFYFPQPLDDFDLGGVTLPAEELARWVRVEQIEG
                     PDGHGQHRVQIDENAARNWLAQFGAQFYREPVNARYYFNDDTRQLVLVAPHVNGRELD
                     VEATLQRLVAQLNSGNHAVPIILRDITPLAHSGATAAELGITELITETTT"
     gene            complement(1220..1933)
                     /locus_tag="PK829_08980"
     CDS             complement(1220..1933)
                     /locus_tag="PK829_08980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41389.1"
                     /translation="MQTTIALLLAAVLLVGGLAWLLHWLLIRTEGVFLGRRVVVWLYD
                     LAAARYDAIKQYDPDTESAFVAWPLSRRLKGRPTPVVLDVATGTGRLPLFLLAEPTFN
                     GRVIGLDASAGMLAQARAKLAAYDDRAALVRGSASDLPFPANAFAAVTCLEALEFLPD
                     DAAALREMVRVLAPGGVLLTTRRQGAEAGYFLGRARGREELEALLGGLGLIDVRSQPW
                     QVEYELVWAAKGPARVSQI"
     gene            complement(2360..3025)
                     /locus_tag="PK829_08985"
     CDS             complement(2360..3025)
                     /locus_tag="PK829_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HOU41390.1"
                     /translation="MTTPVETSAVPARRPRRLSRETQRRWLRIAYHAIGLFPLAWLLF
                     DYWFGLLGPDPIRAMTLRTGKAAIILLTLSLAGTPVATVFGWKDALNLRRPFGLYAFL
                     YVCIHLAIFVGLDYGFLFKLIVEEISERRYALVGFSAFLLLVPLALTSTKGWQRRLGK
                     RWKVLHKLAYLIGVLAVVHYIWLVKNAYAQPLLFAGLVGVLLLIRVPAIKTAILRARR
                     RRA"
     gene            complement(3041..4072)
                     /gene="msrP"
                     /locus_tag="PK829_08990"
     CDS             complement(3041..4072)
                     /gene="msrP"
                     /locus_tag="PK829_08990"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HOU41391.1"
                     /translation="MLIKKRTALDIPSSEITPRSVYLSRRAFITGAAAIAGAAALTAC
                     APKTDLSGVGRAAQMRATAAAAEAAAAAGMPVPPTATPVVVPGQTDELGDPLTSLEAV
                     TGYNNYYEFSTNKEAVAGRAEGFRTSPWQVAVGGLVAKPGVYDIDDLRARFAAEDRVY
                     RLRCVEGWSMVIPWQGFPLAALLRAVEPASDAQYVRFETLYDPDQMPGQNSPFFPWPY
                     VEGLRLDEAMNDLTLMATGLYGEALRPQNGAPIRLVVPWKYGFKSIKSIVKIDLVNEM
                     PESLWMASAPNEYGFYANVNPDVDHPRWSQATERRLGEGGRRPTLLFNGYGEQVASLY
                     TGMDLAANF"
     gene            4229..>4670
                     /locus_tag="PK829_08995"
     CDS             4229..>4670
                     /locus_tag="PK829_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41392.1"
                     /translation="MIANPLLKKLRLPAGGRAAFINDPSGYVDALGLPAGVVVSETVD
                     ERCDFAQIFVRDSGELAAIIDGVLARARPDCVLWFCYPKLSSGVASDLTRDEGWAALT
                     AAGWRGIASVAIDAVWSGVRFRPGSIDGGVAGIDAQYAGGKAALR"
BASE COUNT          799 a         1485 c         1648 g          738 t
ORIGIN      
        1 caggtcgtcg tctcggtgat gagttcggta atgcccaact cggccgccgt cgccccggag
       61 tgggccagtg gggtgatgtc gcgcaggatg atgggcacag cgtggttgcc gctgttcaac
      121 tgggccacca ggcgctgcaa cgtcgcttcc acatccagct cgcggccgtt gacgtgtggg
      181 gcgacgagga ccagctggcg cgtatcgtcg ttgaagtaat agcgggcgtt gaccggctcg
      241 cggtagaact gcgccccgaa ctgggccagc cagttgcggg cggcgttctc gtcgatctgg
      301 acgcggtgct ggccgtggcc atccggcccc tcgatctgtt caacgcgcac ccagcgcgcc
      361 aactcctcgg ccggcaacgt tacgccaccc aggtcaaagt cgtccagcgg ctgcgggaag
      421 tagaagctca ctggctcgga gtaaagttga gccagttgct cagtggtggc ggtatcgtcg
      481 tagacaggcg ggtagatgtc gtggacgagc agttcaacgc gcgcgtcggc tcccgcgcga
      541 ataggttgca gtagttgttc gcgcaagtag gcgcggtcga tgctgcggcc gatctgcgcc
      601 ggggtgtagg ccggcgcgcc gctgtcgttc ggctgccagc tggcgctaac ggcgttgcgc
      661 tccacagttt cgctcagccc ggccaggacg cggtcgagtt gcgcctcgtt gaaaacgacg
      721 ataggggcaa tgccgcggcc gtagtaccag ctctggaagg cgtcttgcag gcggcggaat
      781 ggcccgccag aacggccgac gtcgaaggca gccgaggcgg tggcggcggt atcgacggca
      841 atgcccaact gcgccggcgt gaagctccac tcggtgcccg tgcccggatc gaccagcgta
      901 acggtggccg cgccggcgta gggcagtgcc ccactgaggg cggcctcggc ttcggccgtg
      961 gtcttctcgc tcaggtcgat gccggccacg ctgacgccgg tatagacccg gccgctgtag
     1021 cgggcgcggt aggcggtcag gcccagggcc aacaacacag ccaatagcgt cagcccgacg
     1081 atgggcggga tgaggaagta gccgaggttg cgggcgcggg cggcgcgtgg ggggcgaggt
     1141 gagattgcct gcataattgc tgtgtctcgc tgcgaaccgg ctgaattcta acatgctcgc
     1201 cgcgcggggc gacccttact cagatttgac tcacccgcgc cggccccttg gcggcccaaa
     1261 ccagctcata ctcgacctgc cagggctggc tgcgcacgtc gatcagcccc agcccgccca
     1321 gcagcgcctc cagctcttcg cgtccgcggg cgcggccgag gaagtagccc gcctccgccc
     1381 cctgccggcg cgtggtcagc agcacgccgc ccggcgccag cacacgcacc atctcgcgca
     1441 gcgcggcggc gtcgtcgggc aggaactcca gcgcctccag acaggtcacg gcggcaaagg
     1501 cgttggcggg aaagggcagg tcgctggccg agccgcgaac gagggccgcg cgatcatcgt
     1561 aggcggccag cttggcccgc gcctgcgcca gcattcccgc cgaggcatcg aggccgatga
     1621 cgcggccgtt gaaggtcggc tcggccagca gaaacaaggg cagccgcccc gtgcccgtgg
     1681 ccacgtcgag cacgaccggc gtgggccgcc ccttcagccg ccggctgagc ggccaggcga
     1741 cgaaagcgct ctctgtgtcg ggatcgtact gcttgatggc gtcgtagcgc gccgcggcca
     1801 ggtcatagag ccagacgaca acccgccgcc ccaggaagac gccctccgtg cgaatgagca
     1861 accagtggag cagccaggcc agcccgccca ccagaagcac ggccgccagt agaagcgcga
     1921 tcgtcgtttg cattgccgca aatgctcatg caaagacgcg cgaaaggcaa ggaattggcc
     1981 accttgcaga ccgtggcgcg attctccaga atcgcgtgcc caagagggcg attcaggaga
     2041 atcgcgccac gggtggtctt ctggtgcgcc gaagagggcg attcaggaga atcgcgccac
     2101 gggtggtctt ctggtgcacc gaagagggcg attcggcgga atcgcgccac aggtagtctt
     2161 ctggtgcgcc gaagagggcg attcaggaga atcgcgccac aggtggtctt ctggtgcgcc
     2221 gaagagggcg attcaggaga atcgcgccac gggtggtctt ctggtgcacc gaagagggcg
     2281 attcaggaga atcgcgccac gggtggtctt ctagtgcgcc ggagagggcg attcaggaga
     2341 atcgcgccac gggtggttct caggcgcgcc ggcggcgggc gcgcaggatg gccgtcttga
     2401 tggccgggac gcgaatcagc agcagcacgc ccaccaaccc agcgaagagc aacggctggg
     2461 cgtaggcgtt cttgaccagc cagatatagt ggacgacggc cagcacaccg atgagataag
     2521 ccagcttgtg gagcaccttc cagcgcttgc ccaagcggcg ctgccagcct ttggtcgaag
     2581 tgagggccag cggcacgagg agcaggaagg ccgagaaacc gaccagggcg tagcggcgct
     2641 cagagatctc ctcgacgatg agcttgaaca ggaagccgta gtcaagcccg acgaagatgg
     2701 ccagatggat gcagacgtag aggaaggcat agaggccaaa cggccggcgc aggttcaggg
     2761 cgtccttcca gccgaagacc gtggccaccg gcgtgcccgc cagcgagagc gtcagcagga
     2821 tgatggccgc cttgcccgtg cgcagggtca tggcgcggat cgggtctggg ccgagcaggc
     2881 cgaaccagta gtcgaacagc agccaggcca gggggaacag gccgatggcg tggtaggcga
     2941 tgcgcagcca gcggcgctgg gtctcgcgtg agagccgccg cggccggcgg gcgggcacag
     3001 cgcttgtttc aacgggtgtg gtcatattgt catccacggg ttagaagttc gccgccaggt
     3061 ccatgccggt gtagagcgac gccacttgct cgccgtagcc gttgaacagc agcgtcggcc
     3121 ggcggccgcc ctcgccgagg cggcgttccg tcgcctgcga ccagcgcggg tgatcgacgt
     3181 cgggattgac gttggcgtag aagccgtact cgttgggcgc ggaggccatc cacagcgact
     3241 ccggcatctc gttcaccagg tcaatcttga cgatggactt gatgctctta aagccatact
     3301 tccagggcac aaccaggcga atgggcgcgc cgttttgcgg ccgcagggcc tcaccataca
     3361 ggccggtggc catcagcgtc aggtcgttca tggcctcgtc cagccgcaac ccctcgacat
     3421 agggccaggg gaagaagggg ctgttctggc cgggcatctg atcgggatcg tagagcgtct
     3481 cgaagcggac gtattgggcg tcgctggccg gctcgaccgc gcgcagcagc gcggccagcg
     3541 ggaagccctg ccaggggata accattgacc agccctcgac gcagcgcagc cggtagacgc
     3601 gatcctcggc ggcgaagcgc gcgcgcaggt cgtcgatgtc gtagacgccc ggcttggcca
     3661 ccagcccacc gacggccacc tgccacgggg aagtgcggaa gccctcggcc cgcccggcga
     3721 cggcctcctt attggtggag aactcgtagt aattgttgta gccggtgacc gcctccaggc
     3781 tggtcagcgg gtcgcccagc tcatcggtct ggccgggcac gacgacgggc gtagccgtgg
     3841 ggggcacagg catgcccgcg gcggcggcag cttcggcggc ggcggccgtg gcccgcatct
     3901 gggcggcccg gccaacgccg ctcagatcgg tctttggcgc gcaggcggtt agggccgcgg
     3961 ccccggcgat ggcggctgcg ccggtgatga acgctcgccg cgacaggtac acactccgcg
     4021 gcgtgatttc ggaggatgga atatcaagtg cagttcgctt cttaattagc atagtgattt
     4081 cctcttctat agatggatac gcaggctcgc ttggttcgca tgttggcctc tactataatc
     4141 tcactgtatt aaatcgggct ttagaaaggc ttaccaatcc cttacacatt tacaccccta
     4201 ccccctaccc accgccacgg agacgccgat gatcgccaat cccttgctca agaagctccg
     4261 tctgccagcc ggcgggcgcg ccgccttcat caacgacccg tccggctacg tcgatgcgct
     4321 cggcctgccg gccggtgtcg ttgtcagcga gacagtcgat gagcgttgcg acttcgcgca
     4381 gattttcgtg cgcgacagcg gcgaactggc cgcgatcatc gacggcgtgc tcgcccgggc
     4441 gcggcccgat tgcgtgctat ggttctgcta ccccaagctg tcgtccggcg tcgccagcga
     4501 cctgacgcgc gacgagggtt gggccgcgct gacggccgct ggctggcggg gtatcgcctc
     4561 ggtggccatc gacgcggtct ggtcgggcgt gcgcttccgg ccgggcagca tcgacggcgg
     4621 cgtggcgggc atcgacgcgc agtatgccgg cggcaaggcg gcgctgcggc
//
LOCUS       DAOSMQ010000244         4668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18415_length_4668_cov_4.393887, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000244 DAOSMQ010000000
VERSION     DAOSMQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18415_length_4668_cov_4.393887"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK829_09000"
     CDS             complement(<1..453)
                     /locus_tag="PK829_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU41393.1"
                     /translation="MNKRPIEADGDESALIARAKSDQEAFGQLYERYAQRIYQYFYYR
                     TSSEADAQDLAAKTFMRAWQHIGAYDDRGVPFSAWLYRIAHNLVANWHRDNSRRKVIS
                     LDDIGRWHVSEDGPEASALLSEDRAALLQAVRRLPSRQAAHSLQQRRPV"
     gene            738..1868
                     /locus_tag="PK829_09005"
     CDS             738..1868
                     /locus_tag="PK829_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU41394.1"
                     /translation="MDPYQGKFAHIAGRLGALVLAVAVVVVVALAGCTQPREVEPAAA
                     PPQSGLGAASQATAAAPMLPTPAATLLQPFTPTPATLGAAVDSLQATQTAAVSPPTPE
                     ITIVADTSVAAAPIDVAAPAGPLTQALPTPSGTYSWTLMVPILMYHYISAPPADADVY
                     RIDLSVTPDNFRDQLAWLRDNGYTSIDYYDLTMAIVGYSELPEKPILLTFDDGYLDNY
                     TNAFPLLQEYGFKGTFFVITEFIDSGREGYMTWPMIEEMARAGHRIESHSRTHPDLTE
                     KDRDGLIWELLGSQQTIAAHIGYTPRYFCYPGGDYNEQTIALLRELDFWGAVTTANDT
                     WHGFNERFEWGRLRIRNDTTVAELAQITDLQGTTHGKRPEDQ"
     gene            1883..2248
                     /gene="folB"
                     /locus_tag="PK829_09010"
     CDS             1883..2248
                     /gene="folB"
                     /locus_tag="PK829_09010"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOU41395.1"
                     /translation="MDKVIVRDLLLRGIIGINPDERVKKQDILLNMVIYTDIRPAAET
                     DDITQAANYKAISKRVMEYVEASEHLLVERLATEIARIILTEFPVQRVRVRVEKPGAL
                     RFARSVGVEIDRRRGDFGL"
     gene            2280..2804
                     /gene="folK"
                     /locus_tag="PK829_09015"
     CDS             2280..2804
                     /gene="folK"
                     /locus_tag="PK829_09015"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU41396.1"
                     /translation="MHRVAILLGSNIDKERNLPAAVRLLRQAAEVAAVSPVYETAAVP
                     DDRPRFLNAAVLLLTGQTAAELKDGLLGNIERALGRERALDRNAPRTIDLDIALYDDA
                     VFDYIPADGRARHVPEPDLMRFPHCLQPVADLLPDLPHPETGEPLGELAARVLRDYTA
                     RHGPVLWPRPDIVL"
     gene            complement(2808..>4668)
                     /locus_tag="PK829_09020"
     CDS             complement(2808..>4668)
                     /locus_tag="PK829_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41397.1"
                     /translation="RMARPDLTEFKADEGRLLSLALATGDGDIARHGIASSVGFPNAP
                     MSVWLYALPLAVWPHAYAATLFTGLLNTLAVAGVYWLARRTWGVPAALAAALLLATGP
                     WAILFSRKIWAQNLLPLFVVGWGIGAVLAFVEGRRPFVALHLLCLAVAAQIHPAAVGL
                     APATLLFLVVFRRRVGWGWVLAGGALGALTAAPYLAYLWGEWQAGGLAISTGQLSAAV
                     SLDSPRLALAIAAGHGIEPLAGADFALPLGAVVARWLVVALVLAGIVFAAVRAAREWS
                     RPGAQAGFLVLAWLLAPALLFVWHRTPVYIHYFIAGLPAACLLAGAAVGAALDRRPAW
                     VKTVMWVALLLVAGLQLTGWLGLMRAVAAQPTAGGFGVPLGSKLAAADAARRLLAASG
                     ADEVLLAGDGADPQTDDFPAEFTPLLHGVAMRAVNLNAEAVFPAGAAVVLLGDPAADG
                     PTSTRDLYAAVPGEVATVAVAGRETYRVQALPPASAPPPAVGLPAPALLANFVHIVGH
                     NGLRPVASGAVWDVHWRTADNPDPADYHIFNHLLEATGARVAQADGAAFAARQWRAGD
                     TVISRFVLPLPPDAPLPLSMRVGMYRFPSLESVPVLDAAANPAGEAIAIELSK"
BASE COUNT          803 a         1671 c         1545 g          649 t
ORIGIN      
        1 gaccgggcgg cgctgttgca ggctgtgcgc cgcctgccga gacggcaggc ggcgcacagc
       61 ctgcaacagc gccgcccggt cttcggacaa cagcgccgac gcctccggcc catcttcgct
      121 gacgtgccag cggccgatgt cgtcgagcga gatgaccttg cgccggctgt tgtcgcggtg
      181 ccaattggcg acgagattgt gggcaatgcg gtagagccag gcagaaaagg gcacgccacg
      241 gtcgtcgtac gcgccgatgt gctgccaggc ccgcatgaat gtcttggcgg ccaggtcttg
      301 ggcatcggcc tcgctgctgg tgcggtagta gaagtattgg tagatgcgct gcgcgtagcg
      361 ctcgtagagt tggccgaagg cctcctgatc gctcttggcc cgcgcgatca gcgccgattc
      421 gtcaccgtcg gcctcgattg gtcgtttgtt catgctacgt gcagccagcc atccattctg
      481 agaatcagcg gcagtatagc aaaagccctg ccctctgact aactcgtagc ccgcgccgct
      541 cattggcgga caagcgccgg gttgtgggcc tccctggtca acacagtccg gtcagtggca
      601 acggcgcgac gactgggcag catagcgata ttatagcgtt ggccattcgc tggacaacgc
      661 cgccgggcgc ggccacacca aagacctcta taagcccttc ttgacgcctt ctctgcgctt
      721 ggctattctg agcgacaatg gatccatacc aagggaaatt cgcccacata gccggccgcc
      781 tgggcgcgct cgttctggcg gtcgccgttg tggtggtcgt ggccctcgcc ggttgtacgc
      841 aaccgcgcga agtggaaccg gccgccgcgc caccccagtc cggtctgggc gcggcgtccc
      901 aggccacggc cgccgcgccg atgttgccca cgccggcggc gacgttgctg caacccttca
      961 cgccgacgcc ggcaacgctc ggcgcggcgg tcgattcgct tcaggccaca cagacggcgg
     1021 ctgtctcgcc acccacgccg gagatcacca tcgtggccga tacctcggtc gcggccgctc
     1081 ccatcgacgt agccgccccc gccgggccgc tcacccaggc actgccaacg ccctccggga
     1141 cctatagctg gacgctgatg gtgcccatcc tgatgtacca ctacatcagt gccccgccgg
     1201 ccgacgccga cgtctaccgc atcgacctgt cggtgacacc agataacttc cgcgaccaac
     1261 tcgcctggct gcgcgacaac ggctacacgt ctatcgacta ctacgacctg acaatggcca
     1321 tcgtcggcta ctcggaactg ccggagaagc ccatcctgct gaccttcgac gacggctatc
     1381 tcgacaacta caccaacgcc tttcccctct tgcaggagta cggcttcaag ggcacgttct
     1441 ttgtgatcac tgaattcatc gacagcgggc gcgagggcta catgacctgg ccgatgatcg
     1501 aggaaatggc ccgcgccggc caccgcatcg aatcccactc gcgcacccac cccgacctga
     1561 ccgagaaaga ccgcgatggc ctcatctggg agcttctcgg ctcccaacag accatcgccg
     1621 cccacatcgg ctacacgccg cgctacttct gctatcccgg cggcgactac aacgagcaga
     1681 ccatcgccct gctacgcgaa ctggacttct ggggcgcggt gacaacggcc aacgacacct
     1741 ggcacggctt taacgagcgg tttgagtggg gccggctgcg cattcgcaac gacacgaccg
     1801 tggccgaact ggcccagatc accgatctgc aaggcacaac ccacggcaag cggcctgaag
     1861 atcaataaga ggcacatcac ttatggacaa ggttatcgtc agagacttac tgctgcgggg
     1921 catcatcggc atcaaccccg atgagcgggt caagaagcag gacatcctgc tgaacatggt
     1981 catctacacc gacatccggc ccgcggccga gaccgacgac atcacccagg cggccaacta
     2041 caaggccatc agcaagcgcg tgatggagta cgttgaagcc tcggagcatt tgctcgtcga
     2101 gcggctggcg acggagatcg cccggatcat cctgaccgag ttccccgtcc agcgcgtgcg
     2161 cgtgcgcgtg gaaaagccgg gcgcgctgcg cttcgccagg tcggtcggcg tggagatcga
     2221 ccgccgccgg ggcgacttcg gcctgtagcc ggtcgcggcc catttcattc gctacgccta
     2281 tgcatcgcgt ggccattctg ctgggttcca acatcgacaa ggagcgcaac ctgccggcgg
     2341 cggtgcgact gctgcgccag gcggccgagg tggccgccgt ctcgccggtc tatgagaccg
     2401 ccgccgtccc cgacgaccgg ccgcgctttc tcaatgccgc cgtgctgctg ctgaccgggc
     2461 agacggcggc ggagttgaag gacggcctgt tgggcaacat cgagcgggcg ttgggccgcg
     2521 agcgcgccct cgaccgcaac gcgccgcgca ccatcgacct cgacatcgcc ctctacgacg
     2581 atgccgtctt cgactacatc cccgccgacg gccgcgcgcg ccacgtgccc gaacccgacc
     2641 tgatgcgctt cccccactgc ctgcaaccgg tggccgacct gctgcccgac ctgccccacc
     2701 ccgagacggg cgagccgctg ggcgaactgg ccgcgcgcgt cctgcgcgat tacacggcca
     2761 ggcacggccc tgtcctctgg ccgcggccgg atatcgtcct ctagcctcta cttgctcagt
     2821 tcgatggcga tcgcttcgcc ggcggggttg gccgccgcgt cgagcacggg cacgctctcc
     2881 agcgacggga agcgatacat gcccacgcgc atgctcagcg gcagcggggc gtctggcggc
     2941 aggggcaaga caaagcgact gatgaccgtg tcgccggcgc gccactggcg ggcggcaaag
     3001 gccgcgccgt cggcctgagc cacgcgcgcg ccggtcgctt ccagcaggtg gttgaagatg
     3061 tggtagtcgg ccgggtcggg gttgtcggcc gtacgccagt gcacgtccca gaccgcgccg
     3121 ctcgccaccg ggcgcaggcc gttgtggccg acgatgtgga cgaagttggc cagcagcgcc
     3181 ggtgcaggca ggcccacggc cggcggcggc gcggacgcgg gcggcagagc ctgaacgcgg
     3241 taggtctctc gcccggcgac ggccacggtg gccacttcgc ccggcacggc ggcatagagg
     3301 tcacgtgtgg aagttgggcc gtcggcggcc gggtcgccca gcaagaccac ggccgcgccg
     3361 gccggaaaga ccgcctcggc gttgaggttc acggcgcgca tggccacgcc atgaagcaaa
     3421 ggggtgaact cggccgggaa gtcgtcggtc tgcgggtcgg ccccgtcgcc ggccagtagc
     3481 acctcgtccg cgccggaggc ggccagcaag cggcgcgccg cgtcggccgc ggccagcttg
     3541 ctgcccaggg gcacgccgaa gccgccggcc gtcggctgcg cggccacggc gcgcatcagc
     3601 cccagccagc cggtcagttg tagaccggcc acgaggagca gcgccaccca catcacggtt
     3661 ttgacccacg ccggccggcg gtcgagcgcc gcgcccacgg ccgccccggc caacaggcag
     3721 gccgccggca gcccggcgat gaagtagtgg atgtagactg gcgtgcggtg ccagacaaag
     3781 agcagcgccg gggccagcag ccaggccaac acgaggaagc cggcctgcgc ccccggtcgc
     3841 gaccattccc gcgcggcgcg cacggcggcg aaaacgatcc ccgccaacac cagggccacg
     3901 accagccagc gagccacgac cgcgcccagc ggcaaggcga agtccgcccc ggccagcggc
     3961 tcgatgccgt gtccggcagc gatggccagg gccagccgcg gcgaatcgag cgacacggcc
     4021 gcggagagct gtccggtcga gatggccagg ccgcccgcct gccactcgcc ccagaggtaa
     4081 gccagatagg gcgcggccgt cagcgccccc agcgccccgc cggccagcac ccagccccag
     4141 ccaacgcgcc gccggaagac gacaaggaac agcagcgtcg ccggggccag cccgacggcg
     4201 gcggggtgaa tctgcgcggc gacggccaga cagagcagat gcagggccac aaacggccgc
     4261 cgcccctcca caaaggccag caccgcgccg atgccccagc cgacgacgaa aagcggcaac
     4321 aggttctgcg cccagatttt gcgcgaaaag aggatggccc acggcccggt ggccagcagc
     4381 aacgccgccg ccagcgccgc ggggacgccc cacgtgcgcc gcgctagcca gtagacgccg
     4441 gccaccgcca gggtgttgag caggccggtg aagagcgtcg ccgcgtaggc gtggggccag
     4501 acggccaacg gcagggcgta gagccagacg ctcattggcg cattggggaa gccgacggaa
     4561 ctggcgatgc cgtggcgggc aatgtcgccg tcaccggtgg ccagggccag gcttagcagg
     4621 cggccctcgt cggccttgaa ctcggtcagg tcgggccgcg ccatccgc
//
LOCUS       DAOSMQ010000245         4648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18520_length_4648_cov_3.774222, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000245 DAOSMQ010000000
VERSION     DAOSMQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18520_length_4648_cov_3.774222"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK829_09025"
     CDS             <1..205
                     /locus_tag="PK829_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016866178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOU41398.1"
                     /translation="LTKLVVGEITVVGSRCGPFAPALRLLEQGAVQARPLIEAEYPLS
                     EALVAFEHAARPGVRKVLLRPGP"
     gene            complement(230..1570)
                     /locus_tag="PK829_09030"
     CDS             complement(230..1570)
                     /locus_tag="PK829_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOU41399.1"
                     /translation="MLYKHPHGNVFYRSMNHPRPKIAYSQGIYLYDESGKRYIDGSGG
                     PLVINVGHGRAEIVEAMTRQAQLAAYVHATMFTSEPLEAYAAALAEVTPLPEPRFYFL
                     SSGSEVVEGAIKLARQIQMARGEERRHVVLSRWNSYHGMTLGALAVSGRPGLRSPYLD
                     LFQDTPHIPSPYLYRFPMSGEQLAGRLEEAIVALGAENVAALIAEPMSGASLGAAQPP
                     DDYWPRVRAICDRYGVLLIADEVLVGFGRTGKMWALEHWQTVPDILVTSKGTAGGYFP
                     LGFIAAKGADVEQVRVALGDFNHGGTFSHHAVGAAAGLATLRIMQREKLVANSAAMGA
                     LMGELLHEALDDHPHVGDIRGRGLFWGLELVQDRLTKEPFPRKEGITHRLTTRAFDLG
                     LVIYHSLGCADGVNGDVVMLGPPLIVDEAQVREIVAILAEAIRLELGAPTGAQR"
     gene            complement(1598..2368)
                     /locus_tag="PK829_09035"
     CDS             complement(1598..2368)
                     /locus_tag="PK829_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU41400.1"
                     /translation="MRIARKLLIALLIVLLVALIGGGIAWAVLNGSNAARPEAVAALE
                     PDTSVTVSRPAGEDWYVFTPTAATPTTGLILYPGGFVDPVAYADVARDIAAAGYLVVI
                     DPMPLNLAVTGIESADAIIAAFPDITAWAIGGHSLGGAMAAEYVKNNPGAIAGLALWA
                     AYPAANTDLSGLPLEVVSIYGDADGVASIDDVLGGAARLPAGAQFVLIPGANHTQFGR
                     YGQGLQRGDNPATIDRDAQQAAVVAATLDLLAAIDSQS"
     gene            complement(2375..3142)
                     /locus_tag="PK829_09040"
     CDS             complement(2375..3142)
                     /locus_tag="PK829_09040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOU41401.1"
                     /translation="MDTTNLTASELMIIAASRALRGNRTVFVGVGLPNIACNLARRSH
                     SPDMELVYESGVFGAQPARLPLSIGDPTLVSGATSVMSMADLFMLYLQRGLIDVALLG
                     GAQIDRFGNLNTTVIGDYATPKTRLPGSGGACEIAINARRIFMIMRLSKRAFVSRIDF
                     VTSPGHLDGGDARRRLGMPGLGPDQVITDKALFTFDNPEREMMLTELAPGQTVESIQA
                     AVGWPLRVAPELRQMTPPTAEELAIVREELDPQGLYR"
     gene            complement(3157..4353)
                     /locus_tag="PK829_09045"
     CDS             complement(3157..4353)
                     /locus_tag="PK829_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41402.1"
                     /translation="MPPLDVFGQDYLRLTLAITKHIDGYIDAYYGPEALRQEVNAAPP
                     RPPAGLLDDLARLEDSIPGDDPARAVYLSATLRAIECTLRLLNGETFDYLQEVHRLYD
                     IHPQLTPESAFESAHRQLDAWLPPLPGGDMAARLDAWRQRFEIPAERGLALLELACAE
                     TRRRTTAFIDLPGDESVEVRLTGNQPWSAYNWYLGHGRSLIEFNTDLPLNATALLATF
                     AHEGYPGHHTEAILKEQILYRQRGYAEQAAALLHSPAAVIAEGIATTALDMIFPNGEG
                     HNWTTEVLLPAAGIATEPDTAERLRGIEAAGAALRAVNGNAAILYHTGQLDEAQTRDY
                     IRAYALATPERAAQMFRFCSHPLYRAYSFTYTEGYNLIAASADPVATFRRLLVEQVLP
                     SELAAP"
     gene            complement(4379..>4648)
                     /locus_tag="PK829_09050"
     CDS             complement(4379..>4648)
                     /locus_tag="PK829_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOU41403.1"
                     /translation="PGLIVDAVVHEPYGAHPSYVQGYYDRDNDFYLKWDEWSRDQATT
                     EAWLEEWVYALPDRAAYLEKLGRETLARLAPGALPPDPINYGRYG"
BASE COUNT          723 a         1439 c         1720 g          766 t
ORIGIN      
        1 cctgaccaag ctggtcgtcg gcgaaatcac cgtcgtcggc tcgcgctgcg ggccattcgc
       61 gccggcgttg cggctgctgg agcagggtgc ggtacaggcg cggccgctca ttgaggctga
      121 gtacccgctc agcgaggcgc tggtcgcgtt cgagcacgcg gcgcggccgg gcgtgcgcaa
      181 agtgctcttg cgacccggcc catagacagc ccggccgcgc gccaaccctc taacgttgcg
      241 cccctgtggg cgcgcccaat tccagacgga ttgcctcggc cagaatggcc acgatttcgc
      301 gcacctgcgc ctcgtcgacg atgagtggcg ggccgagcat caccacgtcg ccattaacgc
      361 catcggcgca gccgagtgag tggtagatga ccagccccag gtcgaaggcg cgcgttgtca
      421 ggcggtgggt gatgccctct ttcctgggga acggctcttt ggtgaggcga tcctgaacca
      481 gttccaaacc ccaaaagagg ccgcgcccgc ggatgtcgcc cacgtgggga tgatcgtcca
      541 gcgcctcgtg gagcaactcg cccatgagcg cgcccatcgc ggccgagttc gccaccagct
      601 tctcgcgttg catgatgcgc agcgtggcca gaccggcggc cgcgcccacg gcgtggtggc
      661 tgaacgtgcc gccgtggtta aagtcgccca gggcgacgcg cacctgttcc acgtcagccc
      721 ctttggcggc gatgaagcca agcgggaagt agccgccggc cgtgcccttc gatgtcacca
      781 ggatatcggg cacggtctgc cagtgttcca gcgcccacat cttgcccgtg cgcccaaagc
      841 ccaccagcac ctcatcggcg atcagcagca cgccataacg gtcgcagata gcgcgcacgc
      901 gcggccagta atcatccggt ggctgggccg cgcccagcga cgcgccgctc attggctcgg
      961 cgatgagcgc cgcgacgttc tccgcgccca gggcgacaat ggcctcctct agccggccgg
     1021 ccagctgttc accgctcatg gggaagcgat agaggtaggg cgaggggatg tggggcgtgt
     1081 cctgaaacag gtccaggtag ggcgagcgca gaccgggccg gccgctgacg gccagcgctc
     1141 ccagagtcat gccgtgatag ctattccagc gcgagaggac cacatggcgg cgttcctcac
     1201 cccgcgccat ttggatctgc cttgccagct tgatggcccc ttcgacgacc tcggagccgc
     1261 tgctgaggaa atagaagcgc ggctctggca gaggcgtgac ctcggccagg gcggcggcat
     1321 aggcttccaa cggctcgctg gtgaacatcg tcgcatggac gtaggcggcc agttgcgcct
     1381 gtcgggtcat ggcctcgacg atctcggcgc ggccatgacc gacgttgatc accagcggcc
     1441 cgccggagcc gtcgatgtag cgtttgccac tctcgtcgta gagatagatg ccctggctgt
     1501 aggcgatctt cggccggggg tggttcatgc tgcgatagaa gacgttgccg tgggggtgct
     1561 tatagagcat gggtgcgctc cgccgggaga gccggcatca ggattgggag tcaatagcgg
     1621 ccagcaggtc gagtgtcgcc gcgacgacgg ccgcctgctg cgcgtcgcgg tcgatggtgg
     1681 ccgggttgtc gccgcgctgg aggccctggc catagcggcc gaactgggtg tggttagcgc
     1741 cggggatgag gacgaactgc gcgccggccg gcagacgggc cgcgccgccg aggacatcgt
     1801 cgatcgaggc cacgccgtcg gcatcaccat agatcgacac gacttccagc ggaaggccag
     1861 acagatcggt attcgcggcc gggtaggcgg cccagagggc taggccggcg atcgcccccg
     1921 ggttgttctt gacgtactcc gccgccatcg ccccgcccag cgagtggccg ccgatggccc
     1981 aggcggtgat gtcggggaag gcggcgatga tggcatcggc cgactcgatg cccgtcacgg
     2041 ccaggttgag cggcatcggg tcgatgacga ccaggtagcc cgccgcggcg atgtcgcggg
     2101 cgacgtcggc ataggcgacc ggatcgacga agccgccggg atagagaatc agaccggtgg
     2161 ttggcgtggc agccgtcggg gtgaaaacgt accagtcctc gcccgccggg cggctgaccg
     2221 tcacgctggt gtcgggttcc agggcggcga cggcttccgg ccgggcggca ttgctgccgt
     2281 tgagtacggc ccaggcgatg ccaccgccga tgagcgcgac caggaggaca ataagcaggg
     2341 cgatgaggag tttacgggcg atgcgcatgg ggggttaccg gtagagtcct tgcggatcaa
     2401 gttcctcgcg gacgatggcc agttcctcgg ccgtcggcgg cgtcatctgg cgcagttccg
     2461 gggcgacccg cagcggccag ccgacagccg cctggatgct ctcgacggtc tgcccgggcg
     2521 cgagttcggt cagcatcatc tcgcgctccg ggttgtcgaa ggtgaacagg gccttgtcgg
     2581 tgatgacctg gtcggggccg agaccgggca tgcccaaccg ccggcgagcg tcgccgccgt
     2641 cgaggtggcc gggactggtc acgaagtcga tcctggagac gaaggcgcgc ttcgacaggc
     2701 gcatgatcat gaagatgcgc cgggcgttga tggcgatctc acacgcgccg ccggagccgg
     2761 gcaggcgcgt cttgggggtc gcgtagtcac cgatgaccgt cgtgttgagg ttgccgaagc
     2821 ggtcgatctg cgccccgccc agcagggcca catcgatcag gccacgttgc aggtagagca
     2881 tgaataggtc ggccatgctc atgaccgacg ttgccccgct gaccagcgtc gggtcgccga
     2941 tggacagggg caggcgggcc ggctgcgcgc cgaagacgcc gctttcgtag accagttcca
     3001 tgtcgggcga gtggctgcgg cgggccaggt tgcaggcgat gttgggcagg ccgacgccga
     3061 cgaagacggt gcgattgccc cgtagggcgc gtgaggcggc gatgatcatc agttcggagg
     3121 ccgttaggtt cgttgtgtcc atagtagttt gggcgcttag ggagcggcca gctccgacgg
     3181 cagcacctgc tcgaccagca ggcggcggaa ggtggccacg gggtcggccg acgcggcgat
     3241 gaggttgtag ccttccgtgt acgtaaagga gtaggcgcgg tacaggggat ggctgcaaaa
     3301 gcggaacatc tgcgcggcgc gctcgggtgt agccagagcg taagcgcgaa tgtagtcccg
     3361 cgtctgggcc tcatccagtt ggccagtgtg gtagaggatg gcggcgttgc cgttgaccgc
     3421 gcgcagggcc gcgccggctg cctcgatgcc gcgcaggcgc tcggccgtgt ccggttcggt
     3481 ggcgatgccg gccgcgggta gcaatacctc ggtcgtccag ttgtgcccct cgccgttggg
     3541 gaagatcatg tccaacgccg tggtggcgat gccctcggcg atgacggcgg ccggcgaatg
     3601 caacagcgcc gccgcttgct cggcatagcc gcgctgccgg tagaggattt gctccttgag
     3661 gatggcctcg gtgtgatgac cggggtagcc ttcgtgggca aaggtggcca gcagggcagt
     3721 ggcgttgagg ggcaggtcgg tgttgaactc aatgagcgag cggccgtggc ccaggtacca
     3781 gttgtaggcg ctccagggct ggttgccggt cagacgcacc tcgacgctct cgtcgccggg
     3841 caggtcaatg aaggcggtcg tgcggcggcg cgtctcggcg caagccagtt ccagcagcgc
     3901 cagcccgcgc tcggcgggga tctcgaagcg ctggcgccag gcgtccaggc gcgcggccat
     3961 gtcgccgccg ggcagcggcg gcagccatgc gtctagctgg cggtgggcgc tctcgaacgc
     4021 gctctcaggc gtcaattgtg gatggatgtc gtagaggcgg tggacttctt gcaggtagtc
     4081 gaatgtctcg ccgtttagca gccgcagggt gcactcgatg gcccgcaacg tggccgacag
     4141 gtagacggcg cgggccgggt cgtcgccggg gatgctgtcc tccaggcgag ccaggtcgtc
     4201 tagcaggccg gccggcgggc ggggcggggc ggcgttgacc tcctggcgca gcgcctcggg
     4261 gccgtagtag gcgtcgatgt agccatcgat gtgcttagtg attgccagcg tcaggcgcag
     4321 atagtcctgg ccaaagacat cgagcggggg cataggttcc tctgtatggg gccgcgtctt
     4381 agccgtagcg gccgtaattg atcgggtcgg gcggcagcgc gccgggggcc aggcgggcca
     4441 gcgtctcgcg gccgagcttt tccagatagg ccgcgcgatc gggcagggcg tagacccact
     4501 cttctagcca ggcttcagtg gtggcctggt cgcgcgacca ctcgtcccac ttcaggtaga
     4561 agtcgttgtc gcggtcgtag tacccttgca cgtagctggg atgggcgcca tacggctcgt
     4621 ggacgacggc gtcgacgatc aggccggg
//
LOCUS       DAOSMQ010000246         4622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18665_length_4622_cov_5.053208, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000246 DAOSMQ010000000
VERSION     DAOSMQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18665_length_4622_cov_5.053208"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="PK829_09055"
     CDS             <1..1567
                     /locus_tag="PK829_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41404.1"
                     /translation="SLTADRAGVPFVTVEEGRVLAWSHTDGLQLTSALDGRATLLGSG
                     VMGVNMLPGRLSFEVACLEGSCDVSGAEDTAALRLRNGQQSVIGGDGFAAPPSRLDRA
                     AYVALAPDVLGEANVVAETAVTEEATPAPTETAAPTETPTAAPTDTPAPTARPTTSGS
                     GPQNTTPLGPGALDFEAFGNWVRGDQDNGTFTRSTAQTHGGAASAELAYDFGTAGNDF
                     VVFQQEHAISGEPNVLRLWVYGDGSGHYLNAWIIDANNQTWQVPFGRVTHTGWKQMEA
                     RIDVDQDWPWTQISGPDNGQVDYPIRFRAFVLDDLSNDYVGKGKIYLDDLTVGTGVAG
                     AQPTTAPGATVPPGATTAPGATVSPDATATAPTGQVGAILFTAAGTLYTVDPGGTPIS
                     LGPATADTCSATASAGGVSYDLAPLGSGCAVIGDGLSLCASPNGAYEVLVSPAPAGTT
                     DRPISIRPAGSAESNFIVQTGLNGGQGIAWSPNSSSFLFTSGGDIVQGFANGSYQTIV
                     RGQPALQCPRWVR"
     gene            1626..2963
                     /gene="asnS"
                     /locus_tag="PK829_09060"
     CDS             1626..2963
                     /gene="asnS"
                     /locus_tag="PK829_09060"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOU41405.1"
                     /translation="MTSYFSIERIAAHVGERVTVRGWLQDSTPKGKLLFMRLRDGSGM
                     TQAVAYRPELGDELFEQLKRLGQESSLIVTGTVKANDRAPGVPRGYEIAVESAEVLQD
                     AQDYPITPKDHGVEFLMDNRHLWLRSSRQWAIMRVRATVKRAIIDFLDGRGFVNIDTP
                     IITPSAAEGTSTLFEVAYFDDKAYLAQTGQLYNEANIMALGKVYCFGPTFRAEKSKTR
                     RHLAEFWMVEPEMAFCDLEELMRLEEDFVSAIVSRTLAERANELELLERDVTPLQNVV
                     APFPRISYDEAVQRVLAIREATDDPDLKEQLTIEWGDDFGSPHETELTKQFDRPVFVY
                     GYPTQVKAFYMEPWPGRPEVCKSVDLLAPEGYGEIIGGSERMSDPDVLLAAIRRHELP
                     EANYQWYIDLRRYGSVPHSGFGLGLERTVAWICGLEHIRMTSPFPRTLNRLTP"
     gene            complement(3048..3590)
                     /locus_tag="PK829_09065"
     CDS             complement(3048..3590)
                     /locus_tag="PK829_09065"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU41406.1"
                     /translation="MTFADLAALVAWLADAGVPLAMWGRGGAKTPADLWAELAAGETT
                     LTDDPPRRAVAVVQVLIRRGGRQLVELEQEMADGRRRARGWPPSEKLKGGEAVLGAAR
                     RCLLEELGLAMPESALYESAPPYDRAVDSPSYPGLSTVYHVHSVAVAGDDLPGLPDAD
                     FWLDNTASADPVRRHLWGWR"
     gene            complement(3613..>4622)
                     /locus_tag="PK829_09070"
     CDS             complement(3613..>4622)
                     /locus_tag="PK829_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU41407.1"
                     /translation="LSYTDSQGDPALRAAIAALYDQVSADEVVILGAPEEGIYLTMRT
                     LLEPGDHVVVLTPAYDSLRHLAAHVSGNVTPWPIRAVPGGWALDPAELEQLLRPETRL
                     VVVNFPHNPTGHLPTPAEFAALVDIVRARGAWLLCDEMYRGLETLPADRLPSAADLYE
                     RAIVLAGLSKTHGLPGLRAGWLLVRDPAVRAALINWKHYTTICPPAPTERLATAALLA
                     HAPLVERSRAQIAANLGLAEAFFARHATRFDWRPPRAGSVALVGLDTPSATAYCHDLA
                     RDAGVLLLPGPFLGADDHSVRFGFGRAGFGAALEQYEAYVVGGLAGGDDRRPPTADNF
                     Y"
BASE COUNT          754 a         1652 c         1548 g          668 t
ORIGIN      
        1 cagcctgacc gccgaccgcg ccggcgtgcc gttcgtcacc gtggaagagg ggcgcgtgct
       61 ggcctggagc catacagacg ggcttcagct gaccagcgcc ctcgacggcc gggcgacgct
      121 cctcggctcc ggtgtcatgg gcgtcaatat gttgcccggc cggttgagct ttgaggtggc
      181 ctgcctggag ggcagttgtg atgtcagtgg ggctgaggac acagccgccc tgcggctgcg
      241 caatggccag cagtcggtta tcggcggcga tggtttcgcc gcgccgccgt cccgacttga
      301 ccgcgcggcc tacgtcgccc tggccccgga tgtgctgggc gaggcgaacg tggtggccga
      361 gacggccgtc actgaggaag cgacacccgc gccaaccgag acagccgcgc cgaccgagac
      421 ccccaccgcc gcgcccacgg acacacccgc ccccaccgcc cgcccgacca ccagtggcag
      481 cggcccgcag aacacgaccc ccctcggccc cggcgcgctc gacttcgagg cgttcggcaa
      541 ctgggtgcgc ggcgaccagg acaacggcac gttcacccgt tcgacggccc agacccacgg
      601 cggcgcggct tcggccgagc tggcctacga tttcggcacg gccggcaacg acttcgtggt
      661 cttccagcag gagcacgcca tcagcggcga gccgaacgtg ctgcggctgt gggtctatgg
      721 cgatggttcc ggccattacc tcaatgcctg gatcatcgac gccaacaatc agacctggca
      781 agtgcccttc ggccgcgtca cccacacggg ctggaagcag atggaggcgc gcatcgacgt
      841 ggatcaggac tggccctgga cccagatcag cggccccgac aacggccagg ttgactatcc
      901 catccgcttc cgcgccttcg tgctcgacga cctctcgaat gactacgtcg gaaagggcaa
      961 aatctacctc gacgacctga ccgtcgggac gggcgtggcc ggcgcgcagc cgacgacggc
     1021 ccccggggcg accgtgccgc ctggcgcgac cactgccccc ggcgcgactg tgtcgcccga
     1081 cgctactgct accgccccca ccggccaggt cggggccatc ctcttcaccg ccgcgggcac
     1141 gctctacaca gtcgatcccg gcggcacgcc gatcagcctc ggcccggcca ccgccgacac
     1201 ctgctcggcc acggcctcgg ctggcggcgt cagctacgat ctggcacccc tgggcagcgg
     1261 ctgcgccgtc atcggcgatg gcctgtcgct atgcgcctcg cccaacgggg cctacgaggt
     1321 gctggtctcg cccgcgccgg ccggcacaac cgaccggccc atctctatcc ggccggccgg
     1381 cagcgcggaa agcaacttta tcgtccagac gggcctgaac ggcgggcagg gcattgcctg
     1441 gtcgccgaac agcagttcat tcctgttcac ctctggcggc gacatcgtgc agggcttcgc
     1501 caacggcagc taccagacca tcgtccgcgg ccagccagcg ctgcaatgtc cgcgctgggt
     1561 gcgctgatgc cgccggccgt tttctgctat actcccggcc tgagcactat tcgcgggaga
     1621 catctatgac atcgtacttc tctatcgagc gcatcgccgc ccacgtcggc gagcgcgtca
     1681 ccgttcgcgg ttggctacag gacagcacgc ccaagggcaa gctgctcttc atgcgcctgc
     1741 gcgacggcag cggcatgacc caggccgtgg cctaccgccc ggaactgggc gacgagctgt
     1801 tcgagcaact caagcgcctt ggccaggagt cgtcgctgat cgtcactggc acggtcaagg
     1861 ccaacgaccg cgcccccggc gtgccgcggg ggtatgagat cgccgtcgag tcggccgagg
     1921 tgctgcaaga cgcccaggat taccccatca cgcccaaaga ccacggcgtt gagttcctga
     1981 tggacaaccg ccacctgtgg ctgcgcagca gccggcagtg ggccatcatg cgcgtccggg
     2041 ccacggtcaa gcgggccatc atcgacttcc tcgacggccg cggcttcgtc aacatcgaca
     2101 cgcccatcat cacccccagc gccgccgaag gcacgagcac gctcttcgag gtggcctact
     2161 tcgacgataa agcttacctg gcccagaccg gccagctcta caacgaagcc aacatcatgg
     2221 ccctgggcaa ggtctactgc ttcggcccca ccttccgcgc cgagaagagc aagacccgcc
     2281 gccacctggc cgagttctgg atggtcgagc cggagatggc tttctgtgac ctggaggaac
     2341 tgatgcgcct ggaggaggac ttcgtcagcg ccatcgtgag ccggacgctg gccgagcggg
     2401 ccaacgaact ggagctgctg gagcgggacg taacgccatt gcagaacgtc gtcgccccct
     2461 tcccgcgcat tagctacgac gaggcggtgc agcgcgtcct ggccatccgc gaggcgaccg
     2521 atgacccaga cctaaaggag cagttgacca tcgagtgggg cgacgacttc ggctcgccgc
     2581 acgagacgga actgaccaag cagtttgacc ggccggtgtt cgtctacggc taccccacgc
     2641 aggtcaaggc gttctacatg gagccgtggc cggggcggcc ggaggtctgc aagagcgttg
     2701 acctgctggc cccggagggc tacggcgaga tcatcggcgg cagcgagcgc atgagcgacc
     2761 ccgacgtgct gctggcggcc atccgccgcc acgaactgcc ggaggccaac taccagtggt
     2821 acatcgacct gcgccgctat ggcagcgtgc cccacagcgg cttcggcctg gggctggagc
     2881 gcacggtggc ctggatttgc gggctggaac acatccgcat gaccagcccc ttcccgcgga
     2941 cgctcaaccg gctgacgccg taagaagggg ccaggttcca ggggtcaggt tctaggggct
     3001 agggaagagc gttcttccct ggaacctgga acctggcccc ttcttcttta gcgccagccc
     3061 cacagatgcc gccgcacagg gtcggccgaa gcggtgttgt cgagccagaa gtcggcgtca
     3121 ggtaggccgg gcaggtcgtc gcccgccacg gcgactgagt ggacgtggta gacggtgctg
     3181 aggccggggt aggagggcga atcgacggcg cggtcgtagg gcggcgcgct ttcgtatagt
     3241 gcagattcgg gcatggccaa ccccagttcc tccagcaggc aacgccgcgc cgcgcccagc
     3301 acagcctccc cgcccttcag cttctccgac ggcggccagc cccgcgcccg gcgacggccg
     3361 tcggccatct cctgttccag ttccaccagc tgccgcccgc cgcgccggat gagcacctgg
     3421 acgacggcca cggctcgccg cggcgggtcg tcggtgagcg tcgtctcgcc ggcggccagt
     3481 tcggcccaca ggtcggccgg cgtcttggcc ccgccccggc cccacatcgc cagcggcacg
     3541 cccgcgtccg ccagccaggc caccagggcg gccaaatccg caaacgtcat tgccttttcc
     3601 atatagcagt tatcaataga agttgtcggc ggtcggcggt cggcggtcat ccccacccgc
     3661 caacccgccc accacatagg cctcatactg ctccagcgct gcgccaaacc cggcccggcc
     3721 aaagccgaag cggacactgt ggtcgtctgc gcccaggaag gggccgggca acagcagcac
     3781 cccggcgtcg cgggccaggt cgtggcagta ggccgtggcc gagggtgtgt ccagaccaac
     3841 cagggccacc gagccggcgc gcggcggccg ccagtcgaac cgcgttgcgt ggcgggcaaa
     3901 gaacgcttcg gccagcccca ggttggcggc gatctgcgcc cggctgcgtt cgaccagcgg
     3961 cgcgtgggcc agtagcgccg ccgtggccag ccgttccgtc ggcgcgggcg ggcagatagt
     4021 ggtatagtgc ttccaattaa taagggcggc gcggacggcc ggatcgcgca ccagcagcca
     4081 gcccgcccgc agccccggca ggccgtgagt cttggacagg ccggccagga cgatagcccg
     4141 ttcgtacagg tcagccgccg agggcagccg gtcggccggg agcgtttcca gcccgcggta
     4201 catctcgtcg cacagcagcc aggccccgcg ggcacggacg atgtccacca gagcggcgaa
     4261 ctcggccggg gtgggcaggt ggccggtggg gttgtggggg aagttgacga cgaccaggcg
     4321 cgtctccggt cgcagcagct gttccagttc ggccgggtcg agcgcccaac cgccgggcac
     4381 ggcgcgaatg ggccacggcg tgacgttgcc gctgacgtgc gcggccaggt ggcgcaacga
     4441 gtcataggcc ggcgtcagga cgacgacgtg atcgcccggc tccagcagcg tgcgcatggt
     4501 cagatagatg ccctcttccg gcgcgcccag gatgacgacc tcgtcggcgc tgacctgatc
     4561 atacagcgcg gcgatggccg cccgcagcgc cgggtcgccc tgcgagtcgg tgtagctcag
     4621 gc
//
LOCUS       DAOSMQ010000247         4603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18778_length_4603_cov_5.518909, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000247 DAOSMQ010000000
VERSION     DAOSMQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18778_length_4603_cov_5.518909"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PK829_09075"
     CDS             <1..706
                     /locus_tag="PK829_09075"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU41408.1"
                     /translation="ALWAVDRAARRVSGRRVAAGQPALDPEHAYALALTALLAGFVGA
                     RLMFVLLHWSSYDDNLLGVVWPLNTGYNGVGGALIAAAAGFFYGRWRRLPLWPALDAL
                     APGLLVFLIAVSLADFLGGAGYGSLTNMPWGIDVFGVRRHAVQLYEALVGVVALGAWW
                     AATSARVPVVPGRPFLWATIAYAGGRLLVDAYRENAALMAGGLHVTQVVALAILLAAM
                     ALLAWPARRRESQTAE"
     gene            complement(691..998)
                     /locus_tag="PK829_09080"
                     /pseudo
     CDS             complement(691..998)
                     /locus_tag="PK829_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002751513.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            1049..1285
                     /locus_tag="PK829_09085"
     CDS             1049..1285
                     /locus_tag="PK829_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002790012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOU41409.1"
                     /translation="MQLDRITTDPKRLNGQPCIRGLRLTVRRVLELLALYPNRAELQR
                     EYPELEDEDIRQALAYAAANLEDSIVAFTYEAAS"
     gene            complement(1303..1776)
                     /locus_tag="PK829_09090"
     CDS             complement(1303..1776)
                     /locus_tag="PK829_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41410.1"
                     /translation="MTNTIPVERLTFTLFGLLTEAFESVYGIFLDKGTSLFETLANIT
                     AEEASRATSGRCATLAAQVNHVRFYIDVLEEYMLGTRTEQADWASSWQVGAVTEEEWA
                     ELKTRLRESYTRTRATMEGFDSWDSDDRVGGAFAIVVHTAHHLGEIRQMLCGIRP"
     gene            1939..2706
                     /locus_tag="PK829_09095"
     CDS             1939..2706
                     /locus_tag="PK829_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU41411.1"
                     /translation="MSEQPGYTVKQLADLAGVTPRTLRYYDAIGLLRPSSVGNNGYRR
                     YDEAAALRLQQILFYRELDLGLETIAALLAEPGFDVEAALRQHREAIQQRVDRFHQLI
                     ATIDKTILHLRGQTEMSTDELFAGFDEETQARYEQEAAEMYDPKLVAESSRRWKSYSQ
                     EDKARVMAEGSVVYSDLAALIDRDPADAAVQAVVGRWHQHLRAFYEPTPDILRGLGQA
                     YEEDPRFAAFFAGLHPDLPAFLSRAIEIYSASGAAAR"
     gene            2820..3497
                     /locus_tag="PK829_09100"
     CDS             2820..3497
                     /locus_tag="PK829_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HOU41412.1"
                     /translation="MDSIIATLLPFIIGSAVVPLQIIVVTLLLTSEKRGPLKAIAFVL
                     GMTVARLAQGALFGFVLTGGSGDPADANSPGIVKAVALTVLGILLLVTAYKKWAKEPD
                     PDAPPPKWLTMLDTVTPLRALFLGAGFVLIAPKLWVFILGAISAIDEAQLGRPASTQT
                     FLLFILLAQSLLLIPILIRLVMPRRAQSLLAALADWMKRYNDPIVIAISLIFGALFLY
                     QGLSAFF"
     gene            complement(3502..>4603)
                     /locus_tag="PK829_09105"
     CDS             complement(3502..>4603)
                     /locus_tag="PK829_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU41413.1"
                     /translation="RQAMAEGAVGLSAALIYSPDSYADTTELIALARAAAEYDGLFAA
                     HLRSEGETFLEALDEFLTIVGAAGIRGEIYHLKASGRRNWPKLEAAIARVEAARAAGL
                     AVTADMYTYHASSTGLDAMMPGWVQEGGHRAWVERLRDPDVRARLRREIALPSADWEN
                     SYHEAGDAGKVLCVSFKNDALKPLTGKSLAEIAALRGADPIDTAMDLVIEDDSRVGCV
                     YFTMSEDNVRREIGLPWVSFCSDSASLAPEGIFLRSNPHPRAYGSFARLLGKYVREEK
                     LIPLAEAMRRLSALPADNLRLDRRGRLRPGHYADVVIFDPATIQDHATFDQPHQYATG
                     VRDVLVNGVLVLRDGEHTGATPGRVVDGPGRR"
BASE COUNT          735 a         1502 c         1495 g          871 t
ORIGIN      
        1 ggcgttatgg gcggtggatc gggcggcgcg gcgggtcagc ggccggcgcg tggccgcggg
       61 gcagccggcg ttggacccag agcacgctta cgctctggcc ctgacggcgc tgctggccgg
      121 cttcgtcggc gcgcggctga tgttcgtcct gctgcactgg tcgtcctacg acgataacct
      181 gctgggcgtc gtctggccgc tcaacaccgg ctacaacggc gtgggcggcg cgctcatcgc
      241 cgcggcggcg gggttcttct atggccgctg gcgccgcctg ccgctgtggc cagcgctgga
      301 cgcgctggcc cctgggctgc tggtctttct catcgccgtc agcctggccg actttctggg
      361 cggcgcgggc tacggctcat tgaccaacat gccctggggc atcgacgtgt tcggcgtgcg
      421 gcggcacgcg gtgcagctct acgaggcgct ggtgggggtg gtggcgttgg gggcgtggtg
      481 ggcggccact tcggcgcgcg tgcccgtcgt tcccggccgg ccgttcctct gggcgacgat
      541 cgcctatgct ggcgggcggc tgctggtcga tgcttaccgc gagaacgccg cgctcatggc
      601 cggggggctg cacgtgacgc aggtagtcgc gctggcgatc ctattggcgg cgatggcgct
      661 gctggcatgg ccggcgcggc ggcgcgagag tcagacagcc gagtagaccc gcacctcacg
      721 gttgctctgt tcggctaact catcttcggc cacgtccaga tcatagtcca tctgcaactg
      781 agccagaact gcggcgacat gttgaagtag cgggccagac gcaacgccgt atccgaggta
      841 atgcgccgct tgccctggac gatctcgttg atccgtcgcg ccggcacgcg gatgtccagc
      901 gccaggcggt tttggctgag acccatcggc ctgagaaact cgtgcagcag tatttcgcca
      961 gggtgtatgg atgctattga atcggctaaa gtctgcattg tgtttccaaa tggcgtaaaa
     1021 tgtgattagg gtagcaaggt atatgagtat acaactagat cgcatcacga ccgatcccaa
     1081 acggctgaat ggccagcctt gtatccgcgg tctgcgcctg acagtacggc gcgtgctgga
     1141 gttgctggcc ctctatccca atcgagccga gttgcagcgc gagtatcccg aattagaaga
     1201 cgaagatatt cgccaggcgc tggcctatgc cgcagcgaat ctagaagaca gtattgtcgc
     1261 cttcacctat gaagctgctt cttgaccaag ggcttccaca gttcagggcc taatcccaca
     1321 caacatctgc cgaatctccc ccagatggtg ggccgtgtgc acgacgatgg caaacgcccc
     1381 accgacgcgg tcgtcgctgt cccacgagtc gaagccctcc atcgtcgccc gggtgcgcgt
     1441 gtagctctcg cgtaggcgcg tcttcagctc cgcccactcc tcctccgtca ccgcgcccac
     1501 ctgccagctc gatgcccagt cggcctgctc ggtgcgcgtg cccagcatgt actcctccag
     1561 cacgtcgatg tagaagcgca cgtggttgac ctgcgccgcc agcgtggcgc agcggccgct
     1621 cgtggcccgc gacgcctcct cggcggtgat gttggccagc gtctcgaaca gcgacgtgcc
     1681 cttgtccagg aagatgccgt agacgctctc gaaggcctcg gtcagcaggc caaacagggt
     1741 gaaagtcagc cgttcgacgg gaatggtatt cgtcatggtt acctctctat tcaatgcagt
     1801 ggccactggc agcagtggcc actggcaaca gtggtcaatg gcaatggcgt agaagtatct
     1861 ctgggaaaca tcaatggtca agttgctcca attgctgctt gacattgacg taccgttatg
     1921 gtttagaatc gtgccgatat gagtgagcag ccgggctaca cggttaaaca gttggccgac
     1981 ctcgccggcg tcacgccacg gacgctgcgc tactatgacg ccatcggcct gttgcggccc
     2041 tcctccgttg gcaacaatgg ctatcgtcgc tacgatgagg ccgcggcgct gcgcctccag
     2101 cagattttgt tctatcgcga actcgatctg ggcctggaga cgatcgctgc gctgctggcc
     2161 gagccgggct tcgatgtcga ggcggcgctg cggcaacacc gcgaggctat ccagcagcgc
     2221 gtcgaccggt tccaccagtt gatcgccacg attgacaaga caatactcca tttgcgagga
     2281 cagacagaga tgagcacaga tgaattgttc gcgggcttcg acgaagaaac ccaggctcgc
     2341 tatgagcagg aggccgccga gatgtatgac cccaaactcg tggctgagtc gagccggcgc
     2401 tggaagagct atagtcagga ggacaaggcc cgcgtcatgg ccgaaggcag cgtggtttat
     2461 agcgacctgg ctgcgctgat cgaccgcgac ccggccgacg ccgccgtgca ggccgtcgtc
     2521 ggccgttggc atcaacacct gcgtgccttc tacgagccaa cgcccgatat cctgcgcggg
     2581 ctggggcagg cctatgagga agacccgcgc tttgccgcct ttttcgccgg gctgcatcca
     2641 gacctgccgg cctttctgag ccgggcaatt gagatctaca gcgcttcggg cgcggccgcg
     2701 cgctaattcg gcgtggcccc acgccctgta cgcacaaaga aaccgagttt ctccggggaa
     2761 ctcggttttt ctctttaatc tgctatagtg cgcgcagcat acgtaggcgg gggttaacaa
     2821 tggactcgat catcgctacc ttactcccct tcatcatcgg cagcgccgtt gtgccgctcc
     2881 agatcatcgt cgttaccctg ttgctgacca gcgaaaagcg cggcccgctc aaagccattg
     2941 cctttgtcct gggcatgacc gtggcgcggt tggcccaggg ggcgctgttc ggcttcgtgt
     3001 tgaccggcgg cagcggcgac ccggccgacg ccaacagccc cggcatcgtc aaggcggtgg
     3061 ccctgaccgt gttgggtatc ctgctcctgg ttacggctta caaaaagtgg gccaaagagc
     3121 cggaccccga cgccccgccg cccaagtggc tgacaatgct cgacaccgtg accccgttgc
     3181 gcgctctgtt tctgggcgcg ggcttcgtcc tcattgctcc gaagctgtgg gttttcatcc
     3241 tgggcgcgat cagcgccatc gacgaagcgc aactgggccg cccggcgagc acacagacat
     3301 tcctgctgtt tatcctgctg gcgcagtcct tgctgctcat cccgattctg atccggctgg
     3361 tcatgccccg gcgcgcccag agcctgctcg ccgccctggc cgactggatg aagcgctaca
     3421 acgatccaat tgtcatcgcc atctcgctta tcttcggcgc gctatttctc tatcaaggcc
     3481 tgagcgcctt tttctagcgg ctcatctgcg gcccggccca tcgaccacgc gccccggcgt
     3541 ggccccggtg tgttcgccgt cgcgcagcac gagcacgcca ttcaccagca cgtcgcgcac
     3601 gccggtggcg tactggtggg gctggtcgaa ggtggcgtga tcctggatgg tcgccgggtc
     3661 gaagatgacc acgtcggcgt agtggccggg ccgcaggcgg ccgcggcggt cgagcctcag
     3721 gttgtcggcc ggcagcgccg acagccttcg catcgcctcc gccagcggga tcaacttctc
     3781 ctcgcgcacg tacttgccca gcagccgggc gaagctgccg taggcccgcg ggtgcgggtt
     3841 cgaccgcagg aaaatgccct ccggggccag cgaagccgag tcggagcaga agctgaccca
     3901 gggcaggccg atctcccggc gcacgttgtc ctctgacatg gtgaagtaga cgcagccgac
     3961 gcggctgtcg tcctcgatga ccaggtccat agccgtgtcg atggggtcgg ccccgcgcag
     4021 ggcggcgatc tcggccagcg atttgcccgt cagcggcttg agggcgtcgt ttttgaagct
     4081 aacgcacagc actttgccgg cgtcgcccgc ctcgtgatag ctgttctccc aatcggcgct
     4141 gggcagggcg atctcccggc gcagccgggc gcgcacgtcc gggtcgcgca gccgttcgac
     4201 ccaggcgcgg tggccgcctt cttgcaccca gccgggcatc atcgcgtcca gcccggtgga
     4261 ggaggcgtgg taggtgtaca tgtcggccgt caccgccagc ccggccgccc gcgccgcctc
     4321 cacgcgggcg atggccgcct ccagcttggg ccagttgcgc cggccgctgg ccttcaggtg
     4381 gtagatctcg ccgcggatgc ccgccgcgcc gacaatggtc aggaactcgt ccagcgcctc
     4441 caggaacgtc tcgccctcgc tgcgcaggtg ggcggcgaaa aggccgtcgt attcggccgc
     4501 ggcgcgggcc agggcgatca gctcagtcgt gtcggcgtag ctgtccgggg aatagatgag
     4561 cgccgccgac aggccgacag cgccctcggc catcgcctgc cgc
//
LOCUS       DAOSMQ010000248         4596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18823_length_4596_cov_4.311826, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000248 DAOSMQ010000000
VERSION     DAOSMQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18823_length_4596_cov_4.311826"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="PK829_09110"
     CDS             <1..1816
                     /locus_tag="PK829_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOU41414.1"
                     /translation="NAYADRIRGERAANLEEAAGYLREACLATSPAAGLVALRTARGW
                     ARVALRQGRWDEAAEATRRGRASFEQLARANALRREGESWRGEVQGLAAMAAAALARQ
                     GDARAALLALETGQATALNDALGRGRDRQLLQRLEVESPARYTAYAAAADAVRATQAP
                     GRDAPATPEETAGRQRRAVEAQAALDAAVAAVRALGGGFEALFTPPTFDDVAAAVPPG
                     APLAYLATTAAGSLLLLLWRGGAGVEVEALWAGLDEDALGRLQVRRAIEAGNIGDIVG
                     GLLPAQVFGRGLGAELAAALPRIGADLMAPLAARLRELGATAVTLIPGGRLNLLPLHA
                     ARYAVDGQERAFLDEFAVTYAPSAQAAAESRDRAAGVVAGGGAALVVGNPMPLPAGLR
                     PLHFAREEAEAVAELYGAPPLLATAATLAAVRRALPGARVFHFAGHGLFDPREPLDSG
                     LVMANGERLTLRDVGDSEALAGMRLVVLSACQTGLTDFNDLPDEVIGLPAIFLQAGAA
                     GVVGSLWPVDDRSTALLMTDFHRRMGAGAPPAEALRAAQLALRNMTRRQVSDYYMSHM
                     RMPTEVASAAHAELPPGQDSDRIYAEPYYWAGFTYAGV"
     gene            1821..2606
                     /locus_tag="PK829_09115"
     CDS             1821..2606
                     /locus_tag="PK829_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41415.1"
                     /translation="MNSDAEITHRAATELRPRLAELVGLEQWPAVAARLARLDEEWLA
                     ATTDGQRARLAAQYRDALAAWPAAYERLRAAARSGSLERDTLRGVAVLLESLGDAGGA
                     AGLRAAANDAGDARQIFLHDDKGYSFRLAHLDFRFWNLAEAIAGVLLAVNTLADPTAR
                     PLALAAGVLLIIAGVGKALVREIGVDDTSVFLGLATAAGESRQAAFPAIMAATNAERE
                     RVHLPALSEQQVQRALYVLAEHLGSIEKSGADEWRVVEDFGRV"
     gene            2801..3535
                     /locus_tag="PK829_09120"
     CDS             2801..3535
                     /locus_tag="PK829_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU41416.1"
                     /translation="MERISQIVLIVVGALALVVLTLGLAVSCRQQPPDASATLTPGGT
                     SLSTIPPTWTPLSGADLTATAAAPIPAGQTATATPFGAPPGVATPGVATPTPDTGTPG
                     ATPPSGATPAPVASPTPNAPAAPPAATATAITYVPGATVRHTVNRGEWLLQIARCYGA
                     PYAAVHAANALPNPDYILPGQVIIVPNIGSAGRIIGPPCVQAYFVQAGDTWESLATRF
                     GTTTAILQRANPGALTPGRAIWAPRN"
     gene            3578..>4596
                     /locus_tag="PK829_09125"
     CDS             3578..>4596
                     /locus_tag="PK829_09125"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOU41417.1"
                     /translation="MSSENETLTYERLIPARPADLYRAFTSSTALREWLCDTATTSAR
                     PNGHVFVGWNDGYYATGHFVELLENRVVCFTWQGRGEPRPTQVHVAIQPATEGTALTL
                     THSAIGQGPEWGDKTEEFDRAWRRALENLESVVTTGEDLRVTRRPMLGILPATLTAAE
                     ASRLGLPDAEGVRLSGTVPELGAAAAGLRADDVITAIDGLPTPDINSLRAALRGRNAG
                     DTVVVDFIREGQRHSTGMNLSGRKIPAIPATAEELVDRVAARRAVVRERILAMQELWG
                     EEAAAFHGEHVQMAESWAWPKPTQQVQGRTGVPVLVGGAPGPILFEHIAEFADGWIPI
                     GGAGVA"
BASE COUNT          594 a         1654 c         1741 g          607 t
ORIGIN      
        1 caatgcctac gccgaccgca tccggggcga gcgggcggcc aacctggaag aggccgcggg
       61 ctacctgcgc gaggcctgtc tggccacgtc gccggccgcc ggcctcgtcg cgctacggac
      121 ggcgcgtggc tgggccaggg tggccctgcg gcaggggcgc tgggacgagg ccgccgaggc
      181 cacccggcgc gggcgggcga gctttgagca gttggcccgc gccaacgcct tgcggcgcga
      241 aggcgagtcg tggcgcggcg aggtgcaggg gctggcggcg atggccgccg cggcgctggc
      301 ccggcagggg gatgcgcgcg cggccctgct ggcgctggaa acgggccagg ccacggccct
      361 taatgatgcc ctggggcgcg gccgggatcg gcaactgctc cagcggttgg aggtcgagtc
      421 gccggcccgc tacaccgcct acgcggctgc cgcggacgcg gttcgggcga cgcaagcgcc
      481 cggccgcgac gcgccggcca cgccagagga aacggccggg cggcaacggc gggcggttga
      541 ggctcaggcg gcgctcgacg ccgccgtcgc cgctgtccgc gccctcggcg gcggctttga
      601 ggccctcttc acgccgccaa ctttcgacga cgtggccgcg gccgtgccgc cgggcgcgcc
      661 gctggcctat ctggccacga cggcggccgg gtcgctcctc ctgctgctgt ggcgtggggg
      721 cgcgggggtc gaagtggagg cgttgtgggc cgggctggac gaggacgcgc tggggcggtt
      781 gcaggtacgg cgggcgatcg aggcgggcaa catcggtgat atcgtcggcg ggctgctgcc
      841 ggcacaggta ttcggccggg gattgggggc tgagttggcc gcggcgctgc cgcggatcgg
      901 cgcggacctg atggctccgc tggcggcgcg gttgcgcgaa ctgggcgcga cggcggtcac
      961 gctcatcccc ggcggccggc tgaatctgct gccgctgcac gcggcgcggt acgcggtgga
     1021 cggccaggag cgggccttcc tggacgagtt cgccgtcacc tacgcgccgt cggcccaggc
     1081 ggcggccgaa agccgcgacc gggcggcggg cgtggtcgcc ggcgggggcg cggcgctagt
     1141 ggtgggcaac cccatgccgc tgccggcggg cctgcgcccc ctgcatttcg cccgcgagga
     1201 ggccgaggcc gtggccgagt tgtacggcgc gccgccgctg ctggcgacgg cggcgacgct
     1261 ggcggcggtg cggcgggcgc tgccgggcgc gcgggtgttc catttcgccg ggcatggcct
     1321 gttcgatccg cgcgagccgc tcgattccgg gctggtcatg gccaacggcg aaaggctcac
     1381 cctgcgcgac gtgggcgata gcgaggcgct ggccgggatg cggctggtcg tcctgtccgc
     1441 ctgccagacc ggactgacag actttaacga cttgcccgac gaggtcatcg gcctgccggc
     1501 gatcttcctg caggcgggcg cggccggggt cgtcggcagc ctgtggccgg tggacgaccg
     1561 ctcgacggcg ctgctgatga ccgacttcca ccggcgcatg ggcgcgggcg cgccgccggc
     1621 cgaggcgctg cgggcggcgc aactggcgtt gcggaacatg acccgccgcc aggtatcgga
     1681 ttactacatg tcccacatgc gcatgcccac ggaggtggcc agcgccgccc acgccgaact
     1741 gcctcccggc caggacagcg accgcattta cgccgagccg tattactggg cgggtttcac
     1801 ctacgcagga gtatagctgg atgaacagtg acgccgagat cacccaccgc gcggccacgg
     1861 agctgcggcc gaggctggcc gaactggtcg gtttggagca atggccggcc gtggccgccc
     1921 ggctggcgcg gctggacgaa gaatggctgg cggcgaccac ggacgggcag cgggcgcggc
     1981 tggcggcgca gtaccgcgac gccctggccg cctggccggc cgcctatgag cgcttgcggg
     2041 cggcggcgcg gtccggctca ttggagcgcg acacgctccg cggcgtggcc gtgctgctgg
     2101 agagcctcgg cgacgcgggc ggcgcggccg ggctgcgggc cgcggccaac gacgccggcg
     2161 acgcccggca aatcttcctc cacgatgaca agggctacag cttcaggctg gcccacctcg
     2221 acttccgctt ctggaacctg gccgaggcga tcgccggcgt gctgctggcg gtcaacaccc
     2281 tggcggaccc gaccgcgcgt ccgctggccc tggccgcggg ggtgttgctg atcatcgccg
     2341 gcgttggcaa ggcgttggtg cgcgagatcg gcgtcgatga caccagtgtc ttcctggggc
     2401 tggccacggc cgcgggcgaa tcgcggcagg cggccttccc ggccatcatg gcggccacca
     2461 acgccgagcg cgagcgtgtc cacttgccgg ccctgtccga gcagcaggtt cagcgggcgc
     2521 tgtatgtgct ggcagagcat ttgggcagca tcgagaagag tggcgcggac gagtggcggg
     2581 tcgtggagga cttcgggcgg gtctagcccg ccgcgcccgg tgcgtcgcac tttcttgagt
     2641 gcgtcgcacc ttcttggggg cgcggccggt caagaaagtg ccaggcactg cggcccgctt
     2701 tccctatgcg ctccgcccca catgcgctat gattcgccgc agtgggtagg atcggtggtc
     2761 ggcggccggt ggtcggtaag ccggccgagg gtgatgggcg atggaacgca tctcgcagat
     2821 tgtcctgatc gtggtgggcg cgttggcgct ggtggtgctg acgctcgggc tggcggtgtc
     2881 ctgtcgccag cagccgcccg acgccagcgc gacgctgacc cccggcggta catccctgag
     2941 caccatcccg ccgacgtgga cgccgctttc cggcgccgac ctgaccgcca ccgccgccgc
     3001 gcccatcccc gccggccaga cggccacggc cacgcccttc ggcgcgccac caggtgtcgc
     3061 gacgcccggt gtcgcgacgc ccacgcccga cacgggaacc cccggcgcga cgccgcccag
     3121 cggcgcgacg cccgcgccgg tcgcgtcccc aacgcccaac gcgccggccg cgccgccggc
     3181 ggcgacggcc acggccatca cctacgtccc cggcgcgacg gtgcgtcaca ccgtcaaccg
     3241 cggcgagtgg ctgctgcaaa tcgcccgctg ctacggcgcg ccctatgccg ccgtccacgc
     3301 cgccaacgcc ctgcccaacc ccgactacat cctgcccggc caggtcatca tcgtgcccaa
     3361 tatcggcagc gccggccgca tcatcggccc gccgtgcgtg caggcctatt tcgtgcaggc
     3421 gggcgacacg tgggaatcgc tggcgacgcg ctttggcacg acgacggcca tcctgcaacg
     3481 ggccaacccc ggcgcgctga cccccggccg cgccatctgg gcgccgcgca actgacggcc
     3541 gcgcccggcg cgggccgggc cacgagagga acgacacatg tccagcgaga acgagacgtt
     3601 gacatacgag cggctcatcc ccgcccgccc ggccgatctc taccgggcct tcaccagcag
     3661 cacggcgctg cgcgagtggc tgtgcgacac ggccacgacc tcggcccggc ccaacggcca
     3721 cgtcttcgtc ggctggaacg acggctacta cgccaccggc cacttcgtcg aactgctgga
     3781 gaaccgcgtt gtctgcttca cctggcaggg gcgcggcgaa ccgcggccga cgcaggtgca
     3841 cgtggccatc cagccggcga ccgagggcac ggccctgacg ctgacccaca gcgccattgg
     3901 tcaaggcccg gagtggggcg acaagaccga ggagttcgac cgcgcctggc ggcgggcgct
     3961 ggagaacctc gaatcggtgg tgacgacggg cgaagacctg cgcgtgacgc gccggccgat
     4021 gctgggcatc ctgccggcga cgctgaccgc ggccgaggcc agccggctgg gcctgcccga
     4081 cgccgagggc gtgcgcctga gcggcaccgt gcccgagctg ggggccgcgg cggccgggtt
     4141 gcgggccgat gatgtcatca ccgccatcga cggcctgccc acgcccgaca tcaacagcct
     4201 gcgcgccgcc ctgcgcgggc gcaacgccgg cgacacggtt gtggtcgatt tcatccgcga
     4261 gggccagcgg cattcgacgg gcatgaacct gtcgggccgc aagattcccg ccatcccggc
     4321 cacggccgag gagcttgtcg accgcgtcgc cgcccgccga gccgtcgtcc gcgagcggat
     4381 cttggcgatg caggagctct ggggcgagga ggccgcagcg ttccacggag agcacgtcca
     4441 gatggccgag tcgtgggcct ggcccaagcc cacccagcag gtccagggcc gcaccggcgt
     4501 gcccgtcctg gtgggcggcg cgcccggccc catcctcttc gagcacatcg ccgagttcgc
     4561 cgacggctgg attcccatcg gtggcgctgg cgtcgc
//
LOCUS       DAOSMQ010000249         4583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_18904_length_4583_cov_5.288869, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000249 DAOSMQ010000000
VERSION     DAOSMQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18904_length_4583_cov_5.288869"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1708)
                     /locus_tag="PK829_09130"
     CDS             complement(14..1708)
                     /locus_tag="PK829_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOU41418.1"
                     /translation="MLRDSFLFRLRPRHLARYRRIAEVLARHGFGAVLTQLGLDDRLN
                     IPRRLLRRGVEESERRPPAVRLRLALEELGPTFIKLGQIASTRPEILPPSVLNELANL
                     HDNVPPDPWEAVLPLIEAELGRPLAEVFAAFDPTPIASASLAQVYPALLPDGTHVVVK
                     VQRPDVERLIDVDLQILGDIAHLMRERLPGFVPFDAVELADEFATALREELDYQREGR
                     NADRFRENFEGADFVRVPRVYWQYTTRRLMVQERLRGIKIDDLDALDAAGRDRQRIAL
                     HASRLIVQEVLEDGFFHADPHPGNMLILTDEVIGLIDFGTVGFLDERDKANLIRLYIA
                     IIQFDAPAAVNQLIRMGIADPAVDEVGLERDLRRLLRRYKGLPLKDISATELLAEIQP
                     IIYEYRLRVPSDYWLLIKTLVILEGVGKHVAPDFDVFEVSGPYVQRFLFKLALPTSWG
                     PDALRALGGWAGFLNDLPGQTGRLLSRIEQGRLELRIQDPATEYLARQVTRGANRIIQ
                     AILLGALIVGLALLLPNLDLTWPWRLLTWIAVLGFSAVVVLALWLLWSIWRSGRRS"
     gene            complement(1828..3354)
                     /locus_tag="PK829_09135"
     CDS             complement(1828..3354)
                     /locus_tag="PK829_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU41419.1"
                     /translation="MNRVQVLLERLNIRATNPGACAGPDAWIDSGGAPLVSYNPTTAE
                     PLAEVVPATPAAVEQVIAAAENGFRAWRELPAPRRGLVVRDLGAAVRQAIEPLGELIT
                     LEMGKIRAEGIGEVQEMIDICDFAVGLSRQLYGLTMPSERPGHRMAEQWHPLGPIGVI
                     TAFNFPIAVWSWNAAIAAVCGDTVVWKPSELTPLTAVAMQHIANSVMADYGLSGIFTL
                     AVGGPDVGRQLVADGRLPLISFTGSTASGRAVAQAVAARLGKSILELGGNNAIVVTAD
                     ADLDLATRAIVFGAVGTAGQRCTSTRRVIVQRPAAAELTARLTRAYAQIRPGDPLAEG
                     TLLGPLVTPTAVAQMEAAVRQAVAEGGEVVFGGRARPDVGPQFVEPTLIRMPAQTDIV
                     RRETFAPILYLLEVDTLDEAIRLHNEVSQGLSSAIFTDSVRTAEAFLAAGGSDCGIAN
                     VNIGTSGAEIGGAFGGEKDTGGGRESGSDAWKAYMRRQTNTINWSKDLPLAQGIVFEG
                     "
     gene            3462..>4583
                     /locus_tag="PK829_09140"
     CDS             3462..>4583
                     /locus_tag="PK829_09140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOU41420.1"
                     /translation="MSVYLLPAVRDWAEWGALFTDARRWRPAIARLWAAELSLAARTG
                     VASVGRVVAGFPGTCAVFILDETAVIKFFPPMVASDFIREREVYRLLAGRVPEMPRLL
                     ADGVLRDRIDWPYLVTSCCPGRDWREARADMPVAEQLAVARTLGERISRVHRTTIPPN
                     DAWPRFVAARLAGVSPALRTVLPEAVAAAAEALLRGTDWFAGWPSLLHADLTEDHALV
                     ECRAGRWALSGLLDWADAEVGDPLYEWVALYFGFCGRDVALFRAFLAGYDPAASLPTR
                     RQLLAYTLLHRFGLSIIAAVLGDEERRDLRGIDELAGRLFPGRAATRTGCIPGGAYAW
                     RQPAGIRGSDPHGGPADPYPGRFHGYPGREYRQRRPAQDD"
BASE COUNT          700 a         1531 c         1621 g          731 t
ORIGIN      
        1 cttgccccac acgttaagag cgccgcccgg agcgccagat cgaccacagc aaccacagcg
       61 ccaggacgac gaccgcggag aagccgagca cggcgatcca cgtcagcagc cgccagggcc
      121 aggtcaggtc gaggttgggc agcagcagcg ccaggccgac gatgagcgct cccagcagaa
      181 tcgcctggat gatgcgattc gcgccccgtg tgacctggcg ggcgaggtac tctgtggccg
      241 ggtcctggat gcgcagttcc agccggccct gctcgatgcg gctcagcagc cgtccggtct
      301 gccccggcag gtcattcagg aagcccgccc aaccgcccag cgcccgcagc gcgtccggcc
      361 cccaactcgt cggcagcgcc agcttgaaca ggaaccgctg cacatagggg ccggacacct
      421 cgaacacatc gaagtcgggg gccacgtgct tgccgacgcc ctccaggata accagtgtct
      481 tgatgagcag ccagtagtcg ctgggaacgc gcaggcggta ctcgtagatg atcggctgaa
      541 tctcggccag cagttccgtg gccgaaatgt ccttcagcgg caggccctta tagcgccgca
      601 acaaccggcg caggtcgcgc tccagcccta cttcgtctac agccgggtcg gcgatgccca
      661 tgcggatgag ttggttgacg gccgcgggcg cgtcgaactg gataatggcg atgtagaggc
      721 ggatgaggtt ggccttgtcg cgctcgtcga ggaaaccgac ggtgccgaaa tcgatcagac
      781 cgatgacctc gtcggtgagg atgagcatgt tgccagggtg ggggtcggcg tggaagaagc
      841 cgtcctccag cacttcctgg acgatcaggc gtgaggcgtg cagagcgatg cgctggcggt
      901 cgcggccggc ggcgtcgagg gcatccaggt cgtcgatctt gatgccgcgc agccgctcct
      961 gaaccatcag ccggcgcgtg gtgtactgcc agtagacgcg gggcacgcgc acgaagtccg
     1021 cgccctcgaa gttctcgcga aagcggtcgg cgttgcgccc ctcgcgttgg taatccagtt
     1081 cttcgcgcag agcggtggcg aactcatcgg ccaactcgac ggcgtcgaag ggcacgaagc
     1141 cgggcagccg ctcgcgcatc aggtgggcga tgtcgcccag gatttgcaga tcgacgtcaa
     1201 tgagccgctc cacgtccggc cgttgcacct tgacgaccac atgtgtcccg tcgggcagca
     1261 aggctgggta gacctgggcc agcgaggccg aggctatcgg cgtggggtcg aaggcggcga
     1321 agacctcggc cagcggccgc cccagttcgg cctcgatcag tggcaagacg gcctcccacg
     1381 ggtcgggcgg aacattgtcg tgcaggttgg ccagctcgtt gagcacggag ggcggcagaa
     1441 tctccggccg ggtgctggcg atctggccca gcttgatgaa cgtcgggccg agttcctcca
     1501 gggccaggcg caggcgcacg gccggcggcc ggcgttcaga ctcctccaca ccgcggcgca
     1561 acaaccggcg ggggatgttg agccggtcgt ccagccccag ttgtgtcagc accgcgccaa
     1621 agccgtggcg ggccagcacc tcggcgatgc gacgatagcg cgccagatga cgcgggcgca
     1681 agcgaaacag gaatgaatct ctgagcaatc gaggcccctt ttctgcgtta ggacaaccac
     1741 ctaatcataa cgcaggctgt aggccctcac caactccgct ttccgctcta cagaatgact
     1801 tagcatgctc tgaggctcgc cgcgccccta gccctcaaag acaatgccct gggccagcgg
     1861 caagtccttc gaccagttga tcgtgttggt ctgacggcgc atgtaggcct tccaggcgtc
     1921 ggagccggac tcgcggccgc cgccggtgtc cttctcgccg ccgaacgcgc cgccgatctc
     1981 cgcgccggac gtgccgatgt tgacgttggc gatgccgcag tcggagccgc cggcggccag
     2041 gaaggcctcg gccgtgcgca cgctgtcggt gaagatagcg ctggacagcc cctggctgac
     2101 ctcattgtgc aggcggatag cctcgtcgag tgtgtccacc tccagtagat agaggatggg
     2161 cgcgaaggtt tcgcggcgga cgatgtcggt ctgcgcgggc atgcggatga gcgtcggttc
     2221 gacgaactgc gggccgacgt cgggccgtgc ccggccgccg aaaaccacct cacctccctc
     2281 ggccacagcc tggcgcacag cagcctccat ctgggccacg gccgtgggcg tcaccagcgg
     2341 ccccagcagc gtgccctcgg ccagcgggtc gccggggcga atctgggcgt aggcgcgcgt
     2401 caggcgggca gttagctccg cggcggcggg gcgctggacg atgacgcggc gggtggaggt
     2461 gcagcgctgt ccggctgtgc ccactgcgcc gaagacgatg gcccgcgtgg ccaggtcgag
     2521 gtcggcgtcg gcggtgacga cgatggcgtt gttgccgccc agttccagga tcgacttgcc
     2581 cagtcgcgcg gccacggcct gggccacggc gcggccggaa gcagtcgagc cggtgaagga
     2641 gatcagcggc agacggccgt cggccacgag ttgccggccc acgtctggcc cgcccacggc
     2701 caacgtgaag atgccgctca gcccgtagtc ggccatgacg ctgttggcga tgtgctgcat
     2761 ggccacggcc gtcagcggcg tcaactccga cggcttccaa acgacggtat cgccgcagac
     2821 ggcggcgatg gccgcgttcc acgaccatac ggcgataggg aagttgaagg cggtgatgac
     2881 gccgatgggg ccgaggggat gccactgttc ggccatgcgg tggcccggcc gctccgaggg
     2941 catggtcagg ccatagagct ggcgcgacag gccaacggcg aagtcgcaga tgtcgatcat
     3001 ctcctgcact tcgccgatgc cctcagcgcg aatcttgccc atctccagcg tgatcagctc
     3061 gcccagtggc tcaatggcct ggcgcacggc cgcgcccagg tcgcgcacga ccaggccacg
     3121 ccgcggcgcg ggcaactcgc gccaggcccg gaaaccgttt tccgcggcgg cgatgacttg
     3181 ttcaaccgcc gccggcgtgg ccgggacgac ctcggccagc ggctcagccg ttgttgggtt
     3241 ataggacacc agcggcgcgc cgccgctgtc gatccaggcg tcggggccgg cgcacgcgcc
     3301 ggggttcgtg gcgcgaatgt tcagtcgttc cagcaggact tgaacgcgat tcatgacttc
     3361 acctcggggc ctttggcggc gggccgcttc cccgcgccgg cgtttgcggc aattataggg
     3421 agtcgccgcc cgcccgccaa aggggcgggc gcgaaccacc catgagtgtc tatcttctac
     3481 ccgccgttcg cgactgggcc gaatggggcg cgctgttcac cgacgccaga cggtggcggc
     3541 cggctatcgc gcggctgtgg gcggctgagc tgtcgctggc ggcacgcacc ggcgtggcca
     3601 gcgtgggccg cgtggtggcc ggtttccccg gcacgtgcgc ggtctttatc cttgatgaga
     3661 cggcggtcat taagttcttc ccgcccatgg tggccagcga cttcatccgc gagcgagagg
     3721 tttaccggct gttggccggg cgcgtgccag agatgccgcg cctgctggcc gatggcgtcc
     3781 tgcgcgaccg catcgactgg ccctacctgg tcacgtcgtg ctgtcccggc cgtgactggc
     3841 gcgaggcgcg ggcggatatg cccgtcgcgg agcaactggc cgtcgcccgg acgctgggcg
     3901 agcgcatcag ccgcgtccac cggacgacca tcccgcccaa cgacgcctgg ccgcgcttcg
     3961 ttgccgcgcg tctggccggc gtttcccccg cgctgcggac ggtcttgccg gaggcggttg
     4021 ccgcggcggc cgaggcgttg ctgcggggga cggactggtt cgccgggtgg ccgagcctgc
     4081 tccatgccga cctgaccgag gatcatgccc tggtggagtg ccgggccggc cgctgggcgc
     4141 tgtcggggct gctcgactgg gccgacgcgg aggtgggcga cccgttgtat gagtgggtgg
     4201 cgctctattt cggcttctgc gggcgcgacg tggccctgtt ccgcgctttc ctggccggct
     4261 atgatccggc cgcgtccttg ccaacgcgcc gccaactgct ggcctacacg ctactccacc
     4321 gcttcgggct gagcatcatc gccgctgtcc tcggcgacga ggagcggcgc gacctgcgtg
     4381 gtatcgatga gttggccggg cggctcttcc ccgggcgggc agccacccgg accggctgca
     4441 tcccgggggg cgcatatgcc tggcggcagc cagccgggat cagggggagt gatccccatg
     4501 gcggccctgc tgacccttat cctgggcgct ttcatggcta tcctggacgg gagtatcgtc
     4561 aacgtcgccc tgcccaggat gat
//
LOCUS       DAOSMQ010000250         4554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19072_length_4554_cov_4.328073, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000250 DAOSMQ010000000
VERSION     DAOSMQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19072_length_4554_cov_4.328073"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PK829_09145"
     CDS             <1..600
                     /locus_tag="PK829_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOU41421.1"
                     /translation="TAGYSVADWERAIRHGVGPNGHSLLFMPSYEFSGLSDEQVAQMI
                     AYLQTAPAVDRIPAEPVLKPLLRALFLMGQLPLLPAEKVDQDMAHVASVPAEASAEYG
                     AYLVTTCTGCHQANLAGGPVPGSAPGDPLSANLTPAGNLAHWTLDDFKHTLRNGVTPE
                     GKALDPAVMPWPLAGQMTDLELEALWLYLQALPPAQTAP"
     gene            623..1117
                     /locus_tag="PK829_09150"
     CDS             623..1117
                     /locus_tag="PK829_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41422.1"
                     /translation="MYHWMVYIHVLATFAFLLTHGVSSVVALRLRRQRDPAVARAWLQ
                     LNASGGFVAVFYGSLLTLLVSGVISGFMGDWWGNGWIWLALALLIGIIVAMFVIGTRH
                     YGRVRRALGMGYFDGRQEQPAGEPAAAEEIDALLANAPAITLAAIGFGGIAVILWLMM
                     FKPF"
     gene            1283..3415
                     /locus_tag="PK829_09155"
     CDS             1283..3415
                     /locus_tag="PK829_09155"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOU41423.1"
                     /translation="MGEAEQLSSLLVSGVATDATAAAESPGRVARLVRAWAGVLFVLT
                     LLNLALAIGYLLATGDRTAFLSHQAQIPFLALIYAGLAVAVVTHRPRNPIGPLFLGVS
                     TSYVLSAWAAGAPLYAALAPNLVPPVLYDLAQWLSNWVWLPAQILPVTFVFLLFPDGH
                     LLSPRWRLVGWAAGLGLLSLMVVLAAHPGPVAAWGTQPNPFGIAGAEGVLTMVQIAGS
                     ALLAAGIVGSLFSVVVRFRRSRGMERAQMKWLVYTAATILAVSVLAIPLWLSGRLATS
                     RAMEWSITLTNVMTLSIAVAATVAIVRYRLYDIDVVINRTLVYGLMTGIVLMIYGVVV
                     GGVGVLLQTETNWLAALLATGLVAVLFQPIHRRLQRGVNRLLYGQRDEPFEVLSQLGQ
                     RLELTLTPDAVYPTIVETVAQALRLPYVALEVSDDVGFQLVESYGRPIAQPATLPLTN
                     QGEIVGRLIVSGRTAREEFSEADRRLLQNIARQAGAAVHNAQLTADLQRSRQQLVTGR
                     EEERRRLRRDLHDGLGPSLASLLLEARVLRRMIRDDPTAAEKLAGEMQGDIRATIDDI
                     RRVVHELRPPALDDLGFVPAINVLAAKIGGADDQNGALRVDVEAPDDLPALPAAVEVA
                     AYRIIQEALANVVHHAGARCATVRLWLESGLQLEVTDDGAGFTGGRLGGLGLRSMRER
                     AAELGGSCTISGSNADGTRVRATLPLEL"
     gene            3420..4064
                     /locus_tag="PK829_09160"
     CDS             3420..4064
                     /locus_tag="PK829_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU41424.1"
                     /translation="MPAVRVLIADDHPLFRKGLRTLLDSMPQMTVIGEATSGQEAVEQ
                     AVALQPDVILLDLQMPAGGGLGAIRELAQRPTNSRILVVTLFDDDESVFTALKAGARG
                     YVLKDADEAEMIRAIQAVAEGEAIFSPAIAARMMHYFAAPPTVRAGDSFPELTERERE
                     ILTLIARGYANAQIAEELTISLKTVRNHASSIFNKLQVAGRAQAAIRAREAGLK"
     gene            4268..>4554
                     /locus_tag="PK829_09165"
     CDS             4268..>4554
                     /locus_tag="PK829_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOU41425.1"
                     /translation="MKRVLKVLGIIVVVLAAVVIILGVGVYVATQRRINKVYAIDVPS
                     LTIPTDAASIEEGHRLTVIRGCIDCHGEDYGGGVFLDAPMVATIYAANLTTG"
BASE COUNT          717 a         1499 c         1471 g          867 t
ORIGIN      
        1 accgccggct acagcgttgc cgattgggag cgggccattc gtcacggcgt cggtcccaac
       61 ggccacagcc tcttgttcat gcccagctat gagttttccg ggctgagcga cgagcaagtg
      121 gcgcagatga tcgcctactt gcagacggcg ccggcggtgg atcgcattcc ggccgaaccg
      181 gtcctcaagc cgctgctccg cgccctgttc ctcatgggcc agttgcctct gctgccggcc
      241 gagaaggtcg atcaggacat ggcccacgtc gccagcgtgc cggccgaggc gtcagccgag
      301 tatggcgctt atctcgtcac aacctgcacc ggttgccacc aggccaacct ggccggcggt
      361 cctgtgcccg gcagcgcgcc cggcgatccg ctttcggcca acctgacacc ggccggcaac
      421 ctggcccact ggacgctgga cgatttcaag cacacgttgc gcaacggcgt taccccggaa
      481 gggaaggcgc ttgacccggc ggttatgccc tggcccctgg ccggacagat gaccgacctg
      541 gaactggaag ccctgtggct ttatctgcaa gcgctgccgc cggcgcaaac ggcgccgtag
      601 cgtccatcac cggaggagac gcatgtatca ctggatggtc tacattcacg tgctggcgac
      661 tttcgccttt ctgttgacgc atggggttag tagtgtggtc gccctgcgct tgcgccgtca
      721 gcgcgacccg gctgtggcgc gggcctggct acaactcaat gccagtggcg ggttcgtcgc
      781 cgtgttctat ggctcgttgc tcaccctgct ggtcagcggc gtgatcagtg gcttcatggg
      841 cgattggtgg ggcaatggct ggatatggct cgcgctggct ttgctcatcg gcatcatcgt
      901 ggccatgttc gttatcggca cccggcacta cggtcgtgtg cgccgggcgc tgggaatggg
      961 ctatttcgac ggccggcaag aacagcccgc cggcgaaccg gccgcggccg aggagatcga
     1021 cgcgctactg gctaacgcgc cggccatcac cctggccgcc attggcttcg gcggcattgc
     1081 cgtcatcttg tggctgatga tgttcaagcc gttctaagac acagtcgctg gcggaggttt
     1141 tcatgaggag cactctcgtc acggttcttg tctgggtggt cgtgttcgat gcccttgcgt
     1201 gggtctttgg cgtcctgccg acaaagtgac aatacgccgg gaacatccga gacagagaac
     1261 catgttacaa tagtgtggca tcatgggtga agctgaacaa ctctcatccc ttctggtctc
     1321 cggggtggcg acagacgcga ccgccgcggc cgaatcgccc ggccgcgtgg cgcggctagt
     1381 acgtgcctgg gccggtgtcc tcttcgtcct gaccctgttg aacctcgccc tggccatcgg
     1441 ttatctgctc gccacgggtg accgcacggc ctttttgtcc catcaggccc agatcccttt
     1501 cctggccctc atctacgccg gcctggcggt ggccgtcgtg acccaccgcc cgcgcaaccc
     1561 gattggcccg ctcttcctgg gcgtcagtac gtcctacgtc ctgagcgcct gggcggccgg
     1621 ggcgccgctc tacgcggcgc tcgcgcccaa tctcgttccg cccgttctgt atgacctggc
     1681 gcaatggctg agcaactggg tctggttgcc ggcgcaaatc ctgcccgtga ccttcgtctt
     1741 tctgctcttc ccggacggcc acttgctgtc gccgcgctgg cggttggtgg gttgggcggc
     1801 cgggctgggc ttgctgtcgc ttatggtcgt cctggcggcg catcccggcc cagtcgccgc
     1861 gtggggcacg cagcccaacc cctttggtat cgccggggcc gagggcgtgt tgacgatggt
     1921 gcagatagct ggcagcgcgc tgctggccgc cggcatcgtc ggttcgctgt tcagcgtcgt
     1981 cgtccgcttc cgccgttcgc ggggcatgga gcgcgcccaa atgaagtggc tggtctatac
     2041 cgccgccacg atcctcgctg ttagcgtcct ggcgatcccg ctgtggcttt ccggtcgcct
     2101 ggccacttcc cgagccatgg agtggagcat caccctgaca aacgtcatga ccctgagtat
     2161 cgccgtggcg gctaccgtag ccatcgtccg ctaccgcctg tacgacatcg atgtcgtcat
     2221 caaccgcacg ctggtctatg gcctcatgac cggtatcgtc ttgatgatct atggcgtggt
     2281 cgtgggcggg gtgggcgttc tgttgcagac ggaaaccaac tggctggcgg cgctcctggc
     2341 gaccgggttg gtcgcggtgc tcttccagcc cattcaccgt cgcctccagc gcggcgtcaa
     2401 ccgcctgctc tatggccagc gcgatgagcc gtttgaagtc ctgtcacaac tggggcagcg
     2461 gctagaactg acattgacgc ccgatgcggt ctacccgaca attgtggaga ccgtggccca
     2521 ggcgctccgg ctgccctacg tcgctctgga agtcagcgac gacgtcgggt ttcaactggt
     2581 tgaatcctac ggccggccga ttgcccagcc ggcaacccta ccgctgacca atcagggcga
     2641 gatcgtcggc cgcctgatcg tcagcgggcg cacggccagg gaggagttct ccgaagccga
     2701 ccggcgattg ctacaaaaca tcgcccggca ggcgggcgcg gccgtccaca acgcgcagtt
     2761 gacggccgac ttgcagcgct cgcggcagca actcgtgacc ggccgcgagg aagaacgccg
     2821 ccgcctgcgc cgcgacctgc acgacggcct tggcccgtcg ctggcctcgt tgctgttgga
     2881 ggccagagtg ctgcggcgca tgattcgcga cgacccaact gcggccgaaa agctggccgg
     2941 cgagatgcaa ggcgacatcc gggcgacgat cgacgacatc cgccgggtgg tgcatgagtt
     3001 gcggccgccg gcgctggatg acctgggctt cgtccccgcc atcaacgtac tggcggccaa
     3061 gatcggcggc gctgacgacc agaacggcgc tctgcgcgta gacgttgagg ccccggacga
     3121 cttgccggcc ttgccggcgg ccgtcgaggt ggcggcctac cggatcattc aggaagcgct
     3181 ggccaacgtc gtccaccatg ccggcgcgcg ctgcgccacg gtgcgcctgt ggctggagag
     3241 cggcctgcaa ctcgaagtga ccgacgacgg cgcgggtttt accggaggcc gtctgggcgg
     3301 gttgggcctg cgctccatgc gcgaacgggc ggccgaactg ggcggcagtt gcacgatctc
     3361 cggctccaac gccgatggca cgcgcgtccg cgccactctg cctttggagc tataacgcta
     3421 tgcccgccgt gcgtgttctc atcgccgatg accatccgtt gttccgcaaa ggcttgcgaa
     3481 ccttgcttga ttcgatgccg cagatgacgg tgataggcga ggcaacttcc gggcaggaag
     3541 cggtcgagca ggcggtcgcc ctgcagccgg acgtcatttt gctcgacctc cagatgccgg
     3601 ccgggggcgg tctgggcgcg attcgggaac tggcccaacg gccgaccaac agccgtattc
     3661 tggtcgtgac tctcttcgat gatgacgagt cagttttcac cgccctgaaa gccggcgcgc
     3721 gcggctacgt cctcaaggat gctgacgaag cggagatgat ccgcgccatc caggccgtgg
     3781 ccgagggcga ggccatcttt agcccggcca ttgccgcgcg gatgatgcac tacttcgccg
     3841 cgccgccaac ggtgcgcgcg ggtgattctt tccccgaact gaccgagcgc gagcgcgaaa
     3901 ttctgaccct catcgcccgc ggctacgcca acgcccagat cgccgaggaa ctgacaatca
     3961 gcctcaagac cgtccgcaac cacgcttcaa gcatcttcaa caagctgcag gtcgccggcc
     4021 gcgcccaggc agccatccgg gcgcgggaag cggggctgaa gtaggcggcg gacgtgactt
     4081 ggtttacagt cccacagtaa tcccctaggc agaatcccgt ccacacatgt ctatacttgc
     4141 ctgtgtcgtc tcaggcgcgg gaccgacacc agcaccactg cccacccgcc cggccggctc
     4201 agcgtgtcaa ttcctgttgg cacgctggcg gcgaacaaat cgttgcacac gaggacggga
     4261 tgtaggtatg aaacgagttc taaaggtact agggattatc gtggtggttc tggcggcggt
     4321 ggtgatcatt ctgggcgtcg gggtttacgt cgccacgcaa cggcgcatca acaaggtcta
     4381 tgccatcgac gtgccgtcgt tgaccattcc aaccgatgcc gccagcattg aggaaggcca
     4441 ccgcctgacg gtcatccgcg gctgcattga ctgtcacggc gaggactacg gcggcggcgt
     4501 gtttctcgac gcgcctatgg tggccaccat ctacgccgct aacctgacaa ccgg
//
LOCUS       DAOSMQ010000251         4546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19120_length_4546_cov_4.510577, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000251 DAOSMQ010000000
VERSION     DAOSMQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19120_length_4546_cov_4.510577"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="PK829_09170"
     CDS             <1..1960
                     /locus_tag="PK829_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOU41426.1"
                     /translation="RYVVEPNELTLETPYIEHNIAFTRAAFALDAIESIPFGTVDQLS
                     PQDLLDNEDVLRNIRLWDYRPLQQTYQQLQGLRPYYAIGEIDIDRYQIDGVQRQVMLA
                     ARELDKARLPAPSWVNRNLEFTHGYGIVMNPVNEVTNEGQPNFFIKDLPPQSRVPSIA
                     VTRPEIYFGELTNDTIYVGSARQEFSYPSGDENIYTNYDGTGGILLDSALKRLAFAMR
                     QSDPNVLLSKDITSTTRVQFRRQIQDRVRELTPFLLLDADPYLVVTDDGRLVWIQDAY
                     TVSDDYPYSTPARLTPRPSVTETLPSGVALPTASRTLNYIRNSVKVAIDAYDGSVVYY
                     LADANDPIIASYARAFPGVFRPLAEMPADLQSHLRYPIDLFWAQARQYLTYHMTDVRA
                     FYNKEDLWQIPSEVVANAEQETEPYYVTLPLPGGTESEYLLILPFSPANKPNMIAWMA
                     ARNDPGHYGELVVYQMDRQSLVFGPLQVEGRIDQEPTISQQFTLWDQRGSSVVRGNLL
                     VLPIHRSFLYVEPVYLLSEANALPELRRIVTASNTGVAMGETLQRSLISLAQSNLNQT
                     TDVATPDTGATDTGAPDTGANTPPASGTPAPTPAAAPVTVEELVAAANAHLQAAEQAQ
                     RDGDWARYGQELAALRQNLAALAALTGQ"
     gene            2152..2838
                     /locus_tag="PK829_09175"
     CDS             2152..2838
                     /locus_tag="PK829_09175"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HOU41427.1"
                     /translation="MFLAHLVGDYVLQWDALAQWKSRELRGVIVHSVVLFAVTALFAL
                     WIDSGWWAGVLLISVSHFTVDAGQFLFRPTISPLLRFLIDQLLHLFFILLALVWGGYL
                     TWGHMGAQVLASARQAPVLTALLGYAFVTMPTWVLLKFVVYGLLKGAPPDFPAGPNKF
                     VGITERVLITTLVLFGQVLLVPLVALPRLVMEWPRVIRGGGDAVYVAELVSSMVLAVA
                     VGLALSTLRF"
     gene            complement(2865..>4546)
                     /locus_tag="PK829_09180"
     CDS             complement(2865..>4546)
                     /locus_tag="PK829_09180"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU41428.1"
                     /translation="PGVETSTGPLGQGIANAVGMAIAERWLAARFNRPDFPLIDHMTY
                     ALVSDGDLMEGISHEACSLAGHLGLGRLVALYDDNHITIDGKTDLAFSEDVLKRFEAY
                     GWHTARVDGQNPTAVGAALAAAVAETERPSIIACRTTIGFGSPNRAGTSKAHGEPLGV
                     DEVRLTKERLGLPVDVDFYVPDAARAFLGEAAAAGAERMAAWVALRRRYEEQYPDEAE
                     TLRGMLEGNYDVALDVDELFTAGKAVATRVASGIVLNAVAPQVPTLLGGSGDLTPSNK
                     TDLKGAADLKRGDFSGRYIRFGVREHAMGGILNGLALHGGVRPYGGTFLVFSDYMRPA
                     IRLAALMEAEVIYVFTHDSIGVGEDGPTHQPVEQLMALRAIPNLVLIRPADPVETVAA
                     WLAALANRKGPVALILTRQNLPWLNTTVADALRGGYVLAEAAGGDGQPVEPQVILIGT
                     GSEVQLAVGARALLAAEGIAARVVSLPSWELFAQQPQEYRESVLPDHVRARVSVEAGV
                     TEGWQRFVGLDGRSVGVDRFGASAPYEVVFEKLGVTAEAVAKTARELVRGQ"
BASE COUNT          736 a         1642 c         1429 g          739 t
ORIGIN      
        1 gcgctacgtc gtcgagccga acgagttgac cctggaaacg ccctacatcg agcacaacat
       61 cgccttcacc cgcgccgcct tcgccctgga cgccatcgaa tcgatcccct tcggcacggt
      121 cgatcaactc tcgccgcaag acttgctgga caacgaggac gtgctgcgca acattcgcct
      181 gtgggactac cggccgctcc aacagaccta ccagcaactc cagggcctgc gcccctacta
      241 cgccatcggc gaaatcgaca tcgaccgcta ccagatcgat ggcgtccagc gccaggtcat
      301 gctggccgcg cgcgaactgg acaaagcgcg tctgcccgcg ccgtcgtggg tcaatcgcaa
      361 cctggagttc acccacggct atggcattgt gatgaacccg gtcaacgagg tgaccaacga
      421 ggggcaaccc aacttcttca tcaaagacct gccgccacag agccgcgtgc cgtccatcgc
      481 ggtcacgcgg ccggaaatct actttggcga actgacgaac gacaccattt acgtcggcag
      541 cgcccgccag gagttcagct atcccagcgg cgacgagaac atctacacga actacgacgg
      601 cacgggcggc atcctgctcg actcggccct caagcggctg gcctttgcca tgcgccagag
      661 cgaccccaat gtgctgctca gcaaggacat caccagcacg acgcgcgtcc agttccgccg
      721 ccagattcag gatcgcgtgc gcgaactgac gccctttctc ctgctagacg ccgatcccta
      781 cctggtcgtc accgacgacg gccgcctcgt gtggattcag gacgcctaca ccgtgagcga
      841 tgactacccc tactccaccc ccgcccgcct gacgccgcgg ccgagcgtga ccgagacgct
      901 gcccagcggc gtggccctgc cgacggcgtc gcgcacactc aactacatcc gtaactcggt
      961 caaggtggcc atcgacgcct acgacggcag tgtcgtctat tacctggccg atgccaacga
     1021 ccccatcatc gccagctacg cgcgcgcctt ccccggcgtc ttccggccgc tggccgagat
     1081 gcccgccgac ttgcaaagcc acctgcgcta tccaatcgac ctgttctggg cgcaggcgcg
     1141 gcagtacctg acctaccaca tgaccgacgt gcgcgctttc tacaacaagg aagacctctg
     1201 gcaaattccc agcgaggtcg tggccaacgc cgagcaggag accgagccat actacgtcac
     1261 cctgccgctg cccggcggca cagaatcgga atacctgctc atcctgccct tcagcccggc
     1321 caacaagccc aacatgatcg cctggatggc ggcgcgcaac gaccccggcc actatggcga
     1381 gctggtcgtc taccagatgg atcggcagtc gctggtcttc ggcccgctgc aagtggaggg
     1441 ccgcatcgac caggagccga cgatctcgca gcagttcacc ctctgggacc agcgcggctc
     1501 cagcgtcgtg cgcggcaatt tgctggtgct gcccatccac cgcagcttcc tctacgtcga
     1561 gccggtctac ctgttgtcgg aggccaatgc cctgccagag ctacgccgca ttgtaacggc
     1621 cagcaacacc ggcgtggcca tgggcgagac gctgcaacgc tctctcatca gcctggcgca
     1681 gagcaacctg aatcagacga cagacgtggc cacgccggac accggcgcaa cggacaccgg
     1741 cgcgccggac accggcgcta acaccccgcc ggccagcggc acgccggccc ccacccccgc
     1801 ggccgcgccg gtgactgtgg aagagcttgt cgccgcggcc aacgcccacc tgcaagcggc
     1861 cgagcaagcc cagcgcgacg gcgactgggc gcgctatggc caggagctgg cggcgctgcg
     1921 gcagaatctg gcggcgttgg cggccctcac gggccaatag ccgcgtgggc ggtggtgagt
     1981 ggcggctgcc ggccgccacc caccggccgc cgtctaaccc acttcttacc ctaactgtgt
     2041 cctgtaacac agcgcggcgc gcccaaaaca gtatgattgt gtttaaatgc agcacccgcc
     2101 tggtagtaag agtttggggt ggcggccgcg ggttaacgga tgattattgc catgtttctc
     2161 gcccatctgg tgggcgatta tgttctacaa tgggacgcgc tggcacagtg gaagagtcgt
     2221 gagctgcgag gagtcattgt ccacagcgtc gtccttttcg ccgtaacggc gctcttcgcg
     2281 ctgtggatcg actccggctg gtgggccggc gtcctgctca tcagcgtcag tcactttacc
     2341 gtcgacgccg gccagttcct tttccgcccg actatttcgc ccctcctgcg ctttctcatc
     2401 gaccaactcc tgcacctgtt tttcatcctt ctggccctgg tctggggcgg ctatctgacc
     2461 tggggccata tgggcgcgca ggtgttggcc agcgcgcggc aggcgcccgt gctgacggcg
     2521 ctgctgggct atgctttcgt caccatgccc acctgggtgc tcctcaaatt cgtcgtctac
     2581 ggccttctca agggcgcgcc gcccgacttc cccgccggcc ccaacaagtt cgtcggcatt
     2641 accgagcgcg tgctcatcac cacgctggtg ctcttcgggc aggtgctgct cgtgccgctg
     2701 gtggccctgc cccggctggt catggagtgg ccgcgcgtca tccgcggcgg cggcgacgcc
     2761 gtctacgtgg ccgaactggt ctccagcatg gtgctggccg tggccgtagg gttggcgctg
     2821 agcacgctcc gcttttagcc gcgccgcgcc ctgtccgtct cgccctactg gccccgcacg
     2881 agttcccgcg ccgttttggc cacggcctcg gccgtcacgc ccagtttctc gaagacaacc
     2941 tcatatggcg ccgacgcgcc aaagcgatcg acgcccacgg aacggccgtc cagccccacg
     3001 aagcgctgcc agccctccgt cacgcccgcc tcgaccgaca cccgcgcgcg gacgtggtct
     3061 ggcaagacgc tctcgcggta ctcctgcggc tgctgggcga acagctccca cgacggcagc
     3121 gagacgacgc gcgcggcgat gccctccgcg gccagcagcg cccgcgcccc cacggccagc
     3181 tgcacctctg accccgtgcc gatgaggatg acctgcggct caaccggctg gccatcaccg
     3241 ccggccgcct cggccagcac gtagccgccg cgcagggcat cggccacggt cgtgttcagc
     3301 cagggcaggt tctggcgcgt caggatgaga gccacggggc ctttgcggtt ggccagcgcc
     3361 gccagccagg ccgccaccgt ctccaccggg tcggccgggc ggatgagcac caggttggga
     3421 atggcccgca gggccatgag ctgctccacc ggctggtggg tcggcccgtc ttcgcccacg
     3481 ccgatgctgt cgtgggtgaa gacatagatg acctcggcct ccatcagcgc cgccaggcgg
     3541 atcgccggcc gcatgtaatc ggagaagacc aggaacgtgc cgccataggg tcgcacgccg
     3601 ccgtgcaggg ccaggccgtt gaggatgccg cccatggcgt gttcgcgcac gccgaagcgg
     3661 atgtagcggc cggagaaatc gccgcgcttc aggtcggccg cgcccttcag gtcggtcttg
     3721 ttcgacggcg tcaggtcgcc ggagccgccc agcagcgtcg gcacttgcgg ggcgacagca
     3781 ttgagcacga tcccggaggc gacgcgggtg gccacggcct tgccggcggt gaacaactcg
     3841 tccacgtcga gggcgacgtc gtagttgccc tccagcatcc cgcgcagcgt ttcggcctcg
     3901 tcgggatact gctcctcata gcggcggcgc agcgctaccc acgcggccat gcgctccgcg
     3961 ccggcggcgg cggcctcgcc caggaaagcg cgcgcggcgt cgggcacgta gaagtccacg
     4021 tccaccggca ggcccagccg ctccttggtc aaccgcacct cgtctacgcc cagcggctcg
     4081 ccgtgggcct tcgaggtgcc ggcgcggttg gggctgccga agccgatggt cgtgcggcag
     4141 gcgatgatcg acggccgctc ggtctcggcc acggctgcgg ccagggcggc gccaacggcc
     4201 gtcgggttct gcccatcgac gcgcgccgtg tgccagccgt aggcctcgaa gcgcttgagc
     4261 acgtcttcgg agaaggccag gtcggtcttg ccgtcgatgg tgatgtggtt gtcgtcgtag
     4321 agcgccacca gcctgcccag gcccagatgc ccggccagcg agcaggcctc gtgggagatg
     4381 ccctccatca ggtcgccgtc gctgaccagg gcataggtca tgtggtcgat gagtgggaag
     4441 tcgggccggt tgaagcgcgc cgccagccag cgctcggcga tagccatgcc gacggcgttg
     4501 gcgatgccct ggcccagtgg cccggtggag gtttccacgc ccggcg
//
LOCUS       DAOSMQ010000252         4491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19419_length_4491_cov_5.213706, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000252 DAOSMQ010000000
VERSION     DAOSMQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19419_length_4491_cov_5.213706"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PK829_09185"
     CDS             complement(<1..759)
                     /locus_tag="PK829_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU41429.1"
                     /translation="MTDTPSFGGEIVWRPTPDDTERAHLTRFMRAHGIGSYDELMARS
                     TSDVAWFTDAVLKYLDIRFRVPYTQVVDLSDGIAWPRWCVGGRLNIADNCVDKWAADP
                     AARDRPALIWEGEEGATRTLTYGDLAAEVNRCANALRALEIGAGDVVGLFMPMTPEIA
                     VALLALAKIGAVALPLFSGYGAGAVASRLADAGAVALFTADGTFRRGRLAPMKTIADE
                     AAVQTPTLRHVIVLRRAGNAVAMTPGRDHWWHELV"
     gene            complement(768..1064)
                     /locus_tag="PK829_09190"
     CDS             complement(768..1064)
                     /locus_tag="PK829_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41430.1"
                     /translation="MNDEVYELKVKPRAMQPVTINVPSDTLEALQRVADNREMSVTAL
                     MKLYIGQGLRQELAKRFGERVLESTASVLARHLESEQEVNSILREIQLESTLRE"
     gene            complement(1057..1350)
                     /locus_tag="PK829_09195"
     CDS             complement(1057..1350)
                     /locus_tag="PK829_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009547685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOU41431.1"
                     /translation="MQVRYRLQGVEFEWDDDKARANYDKHGVTFEEAAEVHFDPFHVS
                     GDATRGEEDRQFIIGYSLSERLLLTVYTERVDNLRIISARPATRAEKRLYEYE"
     gene            complement(1375..2307)
                     /locus_tag="PK829_09200"
     CDS             complement(1375..2307)
                     /locus_tag="PK829_09200"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOU41432.1"
                     /translation="MTQSFVRVTHLSEVDHKRPTYVAIGSFDGVHLGHQLLLRAMVAQ
                     AQQDGARAAALTFFPHPRRVLQTLPPRFYLTTLDDRVHLLAEQGIDLIITHPFDDEVR
                     QIRAAAFVDQLLAALDMRQLWGGNFALGYKREGDVPFLRQQGEEKGFRVVTLDTLVDW
                     NGAPVSSRRVRAALETGDMAEVNGCLGRTYSVRGPVVKGDQRGRTIGFPTANLALWDE
                     LLLPGNGVYATYAHVHGQRYAAATNVGVRPTVNGASLTVEAHLLDFDDDIYGEETRLE
                     FVRRIRPEMKFAGLDALKGQIAADVAAVRHDLLL"
     gene            2427..2990
                     /locus_tag="PK829_09205"
     CDS             2427..2990
                     /locus_tag="PK829_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOU41433.1"
                     /translation="MNATAVVRKRFTTQEYEQIAAAGVFAEDDRVELLEGEIVEMSPL
                     GPQHSACVDRLNRLLQRRVNDAAIVRVQSPVRLGRHSEPQPDVTLLQPRPDFYADGHP
                     EPEDVLLLIEVADSSLSYDRDLKLPLYARAGVAEVWLVGLLAQTVEVFRAPSESGYGE
                     RREARRGESVAALNVPAVVLSVAEILG"
     gene            complement(3055..3927)
                     /gene="truB"
                     /locus_tag="PK829_09210"
     CDS             complement(3055..3927)
                     /gene="truB"
                     /locus_tag="PK829_09210"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOU41434.1"
                     /translation="MTTGLLLIDKPIGPTSHDVVARVRRLAGLRRVGHAGTLDPLASG
                     LLLVGLGPATRFLEYLVGLDKTYETTVRLGVSTTTYDAEGETTAERPVTLDAGQVAAA
                     LEAFRGPLRQRVPPHSAVKRDGRPLYERARRGEALDLPEREVVIYALDLLAFAPPDVT
                     LRVVCSSGTYIRSLAHDLGAALGCGGYVSALRRTAVGAFTVAQAVSLSALTEQTLASH
                     LLPSEAAVAHLPRLDVDEGEAMRLSLGQRLAAPDGALPGEMAVFGPEGRFVGVVAVDG
                     DVLRPRKMLAAIDL"
     gene            complement(3936..>4491)
                     /locus_tag="PK829_09215"
     CDS             complement(3936..>4491)
                     /locus_tag="PK829_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOU41435.1"
                     /translation="IGVTMTRGIADSLLTGIVTDTMGFRTTNVTATTLRAAADLVDCG
                     ADIADISLRALHRRPLATAQMWGLGLGRMKFQDGLLWTSIARDEMRRMGVDGSPSSSG
                     LVNFLADVEGVAIGAVLSEMPNENVRVGMRAVPPYNVAEVAVQLGGGGHALAAGCTLD
                     GPLAQAEATVVAACLDAIRRQRPH"
BASE COUNT          746 a         1457 c         1551 g          737 t
ORIGIN      
        1 gacgagttcg tgccaccagt ggtcgcggcc gggggtcatg gccacggcgt tgccggcgcg
       61 gcgcaggacg atgacgtgac gcagcgtggg ggtttgcacg gcggcctcgt cggcgatggt
      121 cttcatcggc gcgagacggc cgcggcggaa tgtgccgtcg gcggtgaaga gggccacggc
      181 cccggcgtcg gccagccgcg aggccactgc cccggcgcca tagccggaaa agagcggcag
      241 ggccaccgcg ccgatcttgg ccagcgccag cagggccacg gcgatctccg gcgtcatggg
      301 catgaacagg ccgacgacgt cgcccgcgcc gatctccagc gcgcgcaggg cattggcgca
      361 gcggttgacc tcagcggcca ggtcgccgta ggtcagcgtt cgcgtcgcgc cctcctcgcc
      421 ctcccagatc agcgccgggc ggtcgcgcgc ggccgggtcg gcggcccact tatccacgca
      481 gttgtcggca atgttgagcc gcccgccaac gcaccagcgc ggccaggcga tgccgtcgga
      541 cagatcgacg acctgtgtgt agggcacgcg gaagcggatg tcgaggtact tcagcacggc
      601 gtcggtgaac caggccacgt cgctcgtgga gcgggccatc agctcgtcgt agctgccgat
      661 gccgtgggcg cgcatgaagc gcgtcaggtg ggcgcgttcg gtgtcgtccg gcgtcggccg
      721 ccagacgatc tcgccgccga aggaaggagt gtcggtcatg gccggctcta ctccctgagc
      781 gtactttcca gttgaatctc gcgcaggatc gagttgacct cctgttccga ctccagatgg
      841 cgagccagaa cggacgccgt gctctccagt acacgttcgc caaagcgctt ggccaactcc
      901 tggcgcaggc cctgaccgat gtaaagcttc atcaacgcag tgacgctcat ctcgcggtta
      961 tcggcgacgc gctgcagcgc ctccagcgta tcactgggca cgttaatcgt caccggctgc
     1021 atcgcgcgtg gctttacttt cagttcgtaa acttcgtcat tcatactcgt atagcctttt
     1081 ctcggctcgt gttgccggac gggccgagat aatacgcaaa ttgtcaacgc gttccgtgta
     1141 gacagttagc agtagtcgct ccgatagaga gtagccaatg atgaactgac ggtcttcttc
     1201 gcctcgtgtc gcatcgccgg agacgtggaa cgggtcgaaa tggacttcag ccgcttcctc
     1261 aaaggtcacg ccatgtttgt catagtttgc gcgtgcctta tcatcgtccc actcgaattc
     1321 gacgccctgt aatcggtatc gcacctgcat gacgggcctc tttgatgcca gagattatag
     1381 cagtagatcg tgtctgactg cggccacatc ggccgcaatc tgtcccttca gcgcgtccag
     1441 cccggcgaac ttcatctccg gccggatgcg ccggacgaac tccaggcgcg tctcctcgcc
     1501 atagatatcg tcgtcgaagt cgagcaggtg ggcctcgacg gtcagcgacg cgccgttgac
     1561 cgtcggccgc acgccaacgt tggtggccgc ggcatagcgt tggccgtgga cgtgggcata
     1621 cgtggcgtag acgccgttgc cgggcagcag cagttcgtcc cacagggcca ggttggctgt
     1681 gggaaaaccg atcgtccggc cgcgctggtc gcccttgacc accgggccgc ggacgctgta
     1741 ggttcgcccc agacagccgt tgacctcggc catgtcgccc gtctccagcg cggcgcgcac
     1801 acgacggctg ctgactggtg ccccgttcca gtccaccagc gtatccaacg tgacgacgcg
     1861 gaagcccttc tcctcgccct gctgccgcag gaagggcacg tccccttccc gcttgtagcc
     1921 cagggcgaag ttgccgcccc agagctggcg catgtccagc gccgccagca gttgatcgac
     1981 gaaggcagcg gcgcggatct ggcgcacctc gtcgtcgaag gggtgggtga tgatcaagtc
     2041 gatgccctgc tcggccagca ggtgaacgcg gtcatcgagg gtggtcaggt agaagcgcgg
     2101 cggcaacgtc tgcaagacgc ggcggggatg ggggaagaag gtcagcgccg ccgcgcgagc
     2161 gccgtcctgc tgggcctggg cgaccatcgc ccgcagcagt aactggtggc ccagatggac
     2221 gccatcgaaa gagccgatag cgacgtaggt ggggcgtttg tgatcgactt cgctgaggtg
     2281 ggtaacgcga acaaaggatt gagtcatagc agattcagac ggtgtagctg tggacacggg
     2341 gcagattgta tcataaagag ccacagaccg gggtcgaaag cgttcggggc acgcttcatg
     2401 gtataatcct ggcaggagtg tagcctatga atgcaacagc tgtggttcgc aaacggttca
     2461 cgacgcagga gtatgagcaa atcgccgccg cgggcgtatt tgccgaagac gatcgcgtcg
     2521 agttgttgga gggggaaatt gtcgagatga gtccacttgg gccacaacat tccgcctgtg
     2581 tcgatcgcct gaacaggctg ctacagcggc gggtcaacga tgcagcgatt gtccgcgtgc
     2641 aaagccccgt ccggctgggc cggcattccg agccacagcc cgatgtcacc cttttgcagc
     2701 cgcgccccga cttctatgcc gacggtcacc cggagccgga ggatgtgttg ctgctcatcg
     2761 aagtggccga ctcatcgctg agctacgacc gcgacttgaa gctgccgctc tatgctcgcg
     2821 ccggcgtggc cgaggtgtgg ctggtgggcc tgctggccca gacggttgag gtcttccgcg
     2881 cgccaagcga gagcggctac ggcgaacggc gcgaggcgcg gcggggcgag tcggtagcgg
     2941 cgctgaacgt gcccgcggtg gtgttgagtg tggctgagat tctcggctag acaggccacg
     3001 gtgacagacc tcagaggttt gcggcaaact tctgaggtct agacgcggca aacttcagag
     3061 gtcgatagcc gccagcattt tgcgcggccg tagcacatcg ccatcgaccg ccactacccc
     3121 cacaaagcgc ccctccggcc caaacacggc catctcgccc ggcaaggccc cgtccggcgc
     3181 ggccaaacgc tggcccaatg atagcctcat ggcctccccc tcgtccacgt ccagccgcgg
     3241 cagatgggcc acggccgctt ccgaggggag gagatgactg gcgagggtct gttcggtcag
     3301 agcggacagg ctgacagcct gcgccacggt gaacgcgccc acggccgtcc gccgcagggc
     3361 gctgacgtac ccgccgcagc ccagcgccgc ccccaggtcg tgggccagcg agcggatgta
     3421 ggttccggag gagcagacga cacgcagcgt cacatcaggc ggggcaaagg ccagcaggtc
     3481 gagggcgtag atgaccacct cgcgctccgg caggtcgagc gcttcgccgc ggcgggcgcg
     3541 ttcgtagagc ggccggccgt cgcgcttgac ggccgaatgc ggcggcacgc gctggcggag
     3601 ggggccgcgg aaagcctcga gagccgcggc cacttgcccg gcgtcgaggg tcacggggcg
     3661 ctcggccgtc gtctccccct cggcgtcata ggtcgtcgtg ctgacgccca gccgcacggt
     3721 cgtctcatag gtcttgtcca gcccgaccag gtactccagg aagcgcgtgg ccgggccgag
     3781 gccgaccagt agcaggccac tggccagcgg gtcgagcgtg ccggcgtggc cgacgcgccg
     3841 caaccccgcc agccggcgca cgcgggcgac gacatcgtgc gaggttggcc cgattggctt
     3901 gtcgatgagg agcaggccgg tggtcacgcg gcgagtcagt gggggcgctg gcggcggatg
     3961 gcgtcgaggc aggcggcgac gacggtggcc tcggcctgcg ccagcggccc gtcgagcgtg
     4021 cagcccgcgg ccagggcgtg gccgccgcca cccagttgca cggccacctc ggccacgttg
     4081 tagggcggca cagcgcgcat gcccacacgg acgttctcat tgggcatctc gctcagcacc
     4141 gcgccgatgg ctacgccttc cacgtcagcc aggaagttga ccaggccgga gctactgggg
     4201 ctgccgtcca cgcccatccg gcgcatctcg tcgcgggcga tgctcgtcca caacagcccg
     4261 tcctgaaact tcatccgccc caggccgagg ccccacatct gcgccgtggc cagcggccgg
     4321 cggtggaggg cgcgcaggct gatgtcggcg atgtcggcgc cgcaatcgac aaggtcggcg
     4381 gcggcgcgca gggtggtcgc ggtgacgttc gtggtgcgga agcccatcgt gtcggtgacg
     4441 atgccggtca gcaggctatc ggcgatgccg cgggtcatcg tcacgccgat g
//
LOCUS       DAOSMQ010000253         4454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19623_length_4454_cov_4.316436, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000253 DAOSMQ010000000
VERSION     DAOSMQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19623_length_4454_cov_4.316436"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PK829_09220"
     CDS             complement(<1..1157)
                     /locus_tag="PK829_09220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU41436.1"
                     /translation="MRILFLTARLPYPPTRGDRLRAYHFLRVLSREHRITLLSFIGDE
                     HEAGHLSPLRTLCEDIQLIHRAPWQSTLSAAAGLWRPLPLQALYYRSPVMQGAVDRLL
                     AREAFDAVYVHLFRMAQYVSSRFRPYRILDLTDAISGEVVRSLPYREPFWRLVYRLEL
                     PRIQRYEREMVGRFDETWVISETERAAVAAELGSSAPGNGRRPPNLRVVPNGVEAERY
                     RPQVRPASAPVLVFVGHMGVFHNIDAAERLANVILPRVREEIPAARLDIIGAEPAARV
                     RALAGRPGVRVLGHVTDLNAALNESTVFVAPLRFAAGVQNKVLEAMAAGLPVVTTAEV
                     NAGLRATPGEHLLLADDDETLAALTVALLRDPAQCARLAAAGRGFVLRDFR"
     gene            1218..2210
                     /locus_tag="PK829_09225"
     CDS             1218..2210
                     /locus_tag="PK829_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOU41437.1"
                     /translation="MIVSPVLIIGCARSGTTLLYNVLAEVDALWSIGYESKAIIERDH
                     SPAARGWESGALLAADLTADGRERLLRAFDSAAAPGSYWRRVNRLRGALNRTRAYAGL
                     KQRGRAGDGAAAAVPGAGLGLLRAAARLRRRLLPRAQPIRLLEKTPENCLRLPFLQAL
                     FPDARVLFLVRDGRANVHSLLEGWRQPHLFPGYHTPLPVTSPGQSRGRWAFTLIPGWR
                     ELVDRPLEEICAHQWAAGNAAVLDYAAAAGALPILTVRYEALVAEPDAALAAIAGFLS
                     LRPSDIPAFGRGLPEVNVVSRPGAEKWRAEAEAIARVTPILAPVMARLGYPLAP"
     gene            complement(2199..3341)
                     /locus_tag="PK829_09230"
     CDS             complement(2199..3341)
                     /locus_tag="PK829_09230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU41438.1"
                     /translation="MSAPARPRVAILSSVHQALDNRVFYREARTLARAGFDVTLVAPH
                     DHNETRDGVRIWALPRVARSHRPLLWRALGQLARETAADLYHFHDPELLLLSSWLRRA
                     TGRPTIYDIHEANADFIRVKDYLPGVVRTPLAGVFRRLEPRLAAGESGLIFADDAIAA
                     DFTGFDGPTATLFNFPGAELIASGAAVATGREPVVLYLGGMERNRGAALMLEAFARVV
                     AALPTARLLLVGHFMPPALEVEMRADAARRGIGHAVEFAGHVPFERIGDYLARARAGW
                     VTWQAVAKNCKNIPTKLFEYMAYGLPVVSSDLPSTRPFVRPGENGLLAVAGDPAAHAA
                     ALLALLRDPAEAMRLGERGRQRVADEWNWAAMEPRLLALYAAVLGR"
     gene            complement(3338..4393)
                     /locus_tag="PK829_09235"
     CDS             complement(3338..4393)
                     /locus_tag="PK829_09235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU41439.1"
                     /translation="MNIAFLPVYPNPYQHLLAGALARREAMTVAMLPGLPSPAWLRAH
                     RPAGGEPVDILHYHWLYGLYMANWRTPAQALAFLGRFRLARALGYGLVWTAHNVLPHR
                     AAGLRPLHVAIRRLMMAAADAVIVHCEAARRELTERFSRTGPVAVIAHGNYAGVYPTP
                     DRTTARQQLALPPDSFVYLSLGNIAAYKGLQGYIDIFRAAAGANDIAIIAGRNRDAAG
                     VRRLERLAASEPRLRLSAGFVPDEQMGRYLGAADVLVAPFERILTSGSVITALSAGLP
                     VIAPTLGCLPELIGDAGIVYPAAQPGGLAAALAAIKTADRGHMRVRATAIAAGLGWDD
                     IARQTAVVYRAVAARSL"
BASE COUNT          658 a         1512 c         1636 g          648 t
ORIGIN      
        1 cagcggaagt cgcgcaacac gaacccgcgc ccggccgcgg ccagccgcgc gcactgggcg
       61 gggtcgcgca ggagagcgac ggttagcgct gccagcgtct cgtcgtcgtc ggccagcagc
      121 aagtgctcgc ccggcgtggc ccgcaggccg gcgttgacct cggcggtcgt caccacgggc
      181 agcccggccg ccatggcctc cagcaccttg ttctggacgc cggcggcgaa acgcagcgga
      241 gccacgaaca cggtagactc gttcagggcg gcgttgaggt cggtcacgtg ccccaggacg
      301 cgcacgcccg gccgccccgc cagcgctctc acccgcgccg ccggctccgc gccgatgatg
      361 tcgagccggg cggccggtat ctcctcgcgg acgcgcggca ggatgacgtt ggccagccgc
      421 tcggcggcgt cgatgttgtg gaacacgccc atgtggccga cgaagaccag cactggcgcg
      481 ctcgctggcc gcacctgcgg ccggtagcgc tctgcctcga cgccattggg cacgacgcgc
      541 aggttcggcg gccggcggcc attgcccggc gcgctggagc cgagttctgc ggctacagcg
      601 gcgcgctcgg tttcgctgat gacccacgtc tcgtcgaagc ggccaaccat ctcgcgctca
      661 tagcgctgga tacgcggtaa ctctagccgg tagaccagcc gccagaacgg ctcgcggtag
      721 ggcaacgagc ggacgacttc gccggagatg gcgtcggtca ggtcgaggat gcggtaaggg
      781 cggaaacggc tgctgacgta ttgggccatg cggaaaagat ggacgtatac ggcgtcgaac
      841 gcctcgcggg ccagcaggcg gtcgaccgcg ccctgcatga ccggtgagcg atagtagagc
      901 gcttgcagcg gcagtgggcg ccacagcccg gccgcggcgc tgagggtcga ttgccacggt
      961 gcgcggtgga tgagctggat gtcctcacag agagtgcgca acgggctgag gtggcccgcc
     1021 tcgtgttcgt cgccgatgaa cgacaggagc gtgatgcgat gctcacgcga gagcacgcgc
     1081 aggaagtggt aggcgcgcaa tcggtcgcca cgcgtgggcg ggtagggcag tcgcgccgtc
     1141 aggaagagga tgcgcatcgt gtcgccagaa ggctatcggg tttcattctg cgtctctgcc
     1201 gatatattat cctgcttatg atcgtctcgc cagtgctcat catcggttgc gcccgctccg
     1261 gcacgacgct gctctacaac gtcctcgccg aggttgatgc gctgtggtcg atcggctacg
     1321 aaagtaaggc gatcatcgag cgtgaccatt cgccagcggc gcgaggttgg gaatcgggag
     1381 cgctgttggc cgccgacctg accgccgacg ggcgcgagcg gctgttgcgc gccttcgaca
     1441 gcgccgccgc gccgggcagc tattggcggc gggtgaaccg gctgcgcggc gcgctgaacc
     1501 ggacgcgggc ctacgccgga ctgaagcagc gcggccgggc gggggacggg gcggccgcgg
     1561 ctgtgcccgg cgcggggctg gggctgttgc gcgccgccgc ccgcctgcgt cgccgcctgt
     1621 taccacgcgc gcagcccatc cgtttgctgg agaagacgcc ggagaactgc ttgcgcctgc
     1681 ctttcctgca agctctattt cccgacgctc gcgtgctctt cctcgtgcgc gacggccggg
     1741 ccaatgttca ctcgttgctg gagggttggc ggcagccgca cctcttcccc ggctaccaca
     1801 cgccgttgcc ggtcaccagc cccggccagt cgcgcggacg ctgggccttc acgctcatcc
     1861 ccggctggcg ggagctcgtc gaccggccgt tggaggagat ctgcgcccat cagtgggccg
     1921 ccggcaacgc ggccgtgctc gactacgctg ccgcagccgg cgcgctgccc atcctgacag
     1981 tgcgctacga ggcgctagtg gccgaacctg acgccgccct ggccgccatc gccggcttcc
     2041 tgagcctgcg cccgtccgat atccctgcct tcggacgtgg gctgccggag gtcaatgtcg
     2101 tttcccggcc gggggcggag aagtggcggg ccgaggcgga ggccatcgcc cgcgtgacgc
     2161 ccatcctcgc gccggtcatg gcccggttgg gctatccctt agcgccctag cacagccgca
     2221 tagagggcca gcaagcgcgg ctccatggct gcccaattcc actcgtcagc cacgcgctgc
     2281 cggccgcgct cgcccagacg catggcctcg gccgggtcgc gcagcagggc cagcagcgcc
     2341 gcggcgtggg cggctgggtc gccggccacg gccagcagcc cgttctcgcc cggcctgacg
     2401 aagggccgtg tcgagggcag gtcgctgctg acgacgggca ggccataggc catgtactca
     2461 aacagctttg tggggatatt cttgcagttc ttggccaccg cttgccacgt gacccagccg
     2521 gcgcgggcgc gggccagata gtcgccgatg cgctcgaagg gtacatggcc ggcgaactcg
     2581 actgcgtggc cgatgccccg ccgagcggcg tcggcgcgca tctccacctc caacgccggg
     2641 ggcatgaagt ggccaaccag cagcagccgc gccgtgggca gggcggcgac gacccgcgca
     2701 aacgcctcta gcatcagcgc cgcgccgcgg ttgcgctcca tcccgccgag gtagaggacg
     2761 accggctcgc ggcccgttgc caccgccgcg ccgctggcga ttagttccgc gccggggaag
     2821 ttgaacagcg tcgccgtcgg cccgtcgaaa ccggtgaaat cagcggcgat ggcgtcgtcg
     2881 gcgaagatca gcccgctttc gcccgcggcc aggcgcggct ccagccgccg gaagacgccc
     2941 gccagcgggg tgcggacgac gccgggcaga tagtccttga cgcggatgaa gtcggcgttg
     3001 gcctcgtgga tgtcgtagat cgtcggccgt cccgtcgccc gccgcagcca ggaggagagc
     3061 agcagcagtt ccgggtcgtg gaagtggtag aggtcggcgg ctgtctcgcg ggccaattgg
     3121 ccgagcgccc gccagagcag cggccggtgg ctgcgggcca cgcgtggcag tgcccagatg
     3181 cgcacgccat cgcgcgtctc attgtggtcg tggggggcga cgagggttac atcgaagccg
     3241 gcgcgagcca gcgtccgcgc ctcgcggtag aagacgcggt tgtcgagggc ctggtgcact
     3301 gacgagagga tggcgaccct gggcctggcc ggcgcgctca taggctcctc gcggcgacgg
     3361 cgcgatagac gactgccgtc tgccgggcaa tgtcgtccca gcccaggccg gcggcgatgg
     3421 ccgtggcgcg gacgcgcatg tgtccgcggt cggctgtctt gatggctgcc agcgccgcgg
     3481 ccaggccgcc gggctgggcc gccggataga cgatgcccgc gtcgccgatc agttctggca
     3541 ggcagccgag ggtgggggcg atgacgggca ggccggccga cagcgcggtg atgaccgagc
     3601 cgcttgtcag aatgcgctcg aacggggcca cgagcacgtc ggccgcgccc aggtagcggc
     3661 ccatctgctc atcgggtacg aagccggcgc tcagccgcag ccgcggctca ctcgccgcca
     3721 ggcgctcgag ccgccgcacg ccggccgcgt cccggttgcg tccggcgatg atggcgatgt
     3781 cattcgcgcc ggccgcggcc cgaaagatat cgatatagcc ctgtaagccc ttataggcgg
     3841 cgatgttgcc cagcgacaga taaacgaaac tgtccggtgg cagagccagt tgttgccggg
     3901 ccgtggtccg gtcgggtgtt gggtatacgc cagcgtagtt gccgtgggcg atgacagcaa
     3961 cagggccagt gcgcgagaac cgctctgtca gttcgcgtcg tgccgcctcg caatggacga
     4021 tgacggcatc ggccgcggcc atcatcagcc gccggatggc cacgtgcagc ggccgcagcc
     4081 cggcggcgcg gtggggtaag acgttgtggg ccgtccagac gaggccgtag cccagcgccc
     4141 gcgccagccg gaagcgcccc aggaaggcca gcgcctgggc gggggtgcgc cagttggcca
     4201 tgtagaggcc gtagagccag tgatagtgga gaatgtcgac gggctcgccg ccggctggcc
     4261 tgtgggcgcg cagccacgcc ggcgagggca agccgggcag catcgccacc gtcatggcct
     4321 cgcgccgcgc caacgccccg gccagcagat gctggtaggg attggggtag acgggtagga
     4381 aggcaatgtt catggccacg ggcgctaacg catgtacatg tcgcgcaaca gcacttcggc
     4441 ggccgcccgc ggca
//
LOCUS       DAOSMQ010000254         4439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19715_length_4439_cov_4.249088, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000254 DAOSMQ010000000
VERSION     DAOSMQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19715_length_4439_cov_4.249088"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2811
                     /locus_tag="PK829_09240"
     CDS             <1..2811
                     /locus_tag="PK829_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41440.1"
                     /translation="AQDVEGQSPAPGDPPYKGLESFTEADAEHFLGRERLTARLASRL
                     REERFLAIVGASGSGKSSVVRAGLIPALRAGKPLADGGLPPAGSAQWAIRVLTPTAHP
                     LDALAVALTAAATADTAASDAAALSARLAADPTALATAARDLLAKEGRPHLLLAIDQF
                     EEAFSLARDAAERRALFDNLVAAVDPTANHPITIIVVLRADFYARFAEHDGLRELIAR
                     RQEYIGAMSREELFRAIVAPAARGDWKLQEGLVELMLDDAGDEPGALPLLSHALLATW
                     ERRRGRTMTLSAYKESGGVRGAIAKTAESVFQQRLTPAQRPIARAIFVRLTELGDSAD
                     ADTPDTRRRASFSELITRATDPQMLDAVLNILVDARLVITDVVPPTNTQIVEVAHEAL
                     IREWPTLRAWLDQDREGLIRQRQLTLDVSDWLKLGRDPGALYRGARLARTLEWTAAAP
                     DALSVDEAEFLDASRAAAAREARARTMQRALAISSVVLLVLAIVAALLAFDVISLRPA
                     DQMSGDFNIAVAEFAVLNESGELVKGDGGRRVAERLGATLQSEFAQRPEVQVWFDSPT
                     LEQEHNVTIGVVGLPPLRATPPAEASAALNADVVIHGDIRPAGNGNALTLRFYLRPQF
                     EADFSQMVGFHEFTARVPVFDPANPREEVWRELDPLSGALAWLIQGLRQEILGDQAAA
                     LAAFEQAATLAPDSDVVQYFLGQEHFYSAQRAGGDETQLAAAETAFNESLRLNPDNAR
                     ASIGLGSVHFARGQRLLNEAEARGEGGDATALAAVRAEAEAALAAYTAVALRGEQIET
                     YGVPVAGIARVGRAIALRLLGETAFQGGDPAAAEAHIDEAIAALEADAGQLDIDNDPR
                     LAAQSDQALGSIYEWKAFLLGERGAADEAQAARDKALGFYNACVRQGEDFPFDVYLVE
                     HIVGQLCAPRVTALQAPGGEGG"
     gene            2808..3848
                     /locus_tag="PK829_09245"
     CDS             2808..3848
                     /locus_tag="PK829_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41441.1"
                     /translation="MMARALGAGIVLLVVLALAGCGGGEAEPTVLPLSTADNNGAIAT
                     SVQQTIEAIAVAQTVAAMGVGQPTPSPASEVVATAAIVEPSPTAAPLPTAAEAPAGTC
                     QVVSGVNLRPGPGVAYDPPLGNLSVNTQLTPLAYQAVGFPQGPWLQAQVQATGQVGWI
                     SALPQYVQCDFDLATLPPAVFPPTPPPPTAPPPPIAPTTVAATATRPLASAPPDIDND
                     APGGSFPSTGVTGNVIVDPAFLFRMEVFDEAAGNHDGAGIQFVFFDISGNGISYSRQE
                     NNAGYCVFGGGEPTCNPWPTDDQGRYIWGEGGDIVKTGDYFASITVTAENPDPEFNGV
                     WNWNFSFHVTVP"
     gene            3874..4431
                     /locus_tag="PK829_09250"
     CDS             3874..4431
                     /locus_tag="PK829_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41442.1"
                     /translation="MNKMGRITVGLLALIVLATAVAACGQAAAPAPTTTPPEAIAAGP
                     ASDRPPDVDNDAPAGECQDTTNILSRVDRAPNALFRAVALDNRVGGNDGDGIVGVRFT
                     VTGDNLSFVKDELTAPYCAFGGNEPDCGVWPRDEAGRYTWGAGGSLVQPGRFHVMVEV
                     VAGQPDSATGRAGCDWEFDMQVSMP"
BASE COUNT          650 a         1592 c         1531 g          666 t
ORIGIN      
        1 gctcaggatg tcgagggcca gtcacccgcg cccggcgacc cgccctataa gggtttggag
       61 tcgttcaccg aggccgacgc cgagcacttc ctgggccgcg aacggctgac ggcgcggctg
      121 gccagtcgcc tgcgcgaaga gcgcttcctg gccattgtgg gcgcgtcggg cagcggcaag
      181 tcgtcggtcg tgcgggccgg gctcatcccc gccctgcggg cgggcaagcc cctggccgat
      241 gggggtctgc cgccggccgg ctcggcgcag tgggccattc gcgtgctgac gcccacggcc
      301 cacccgctcg acgccctggc cgtcgccctg accgccgcgg cgacagccga caccgcggcg
      361 tccgacgctg cggcgctctc ggcccgcctg gccgccgacc ccacggccct ggccacggcc
      421 gcccgcgacc tgctggccaa agagggccgg ccccatctgc tgctggcaat cgatcagttc
      481 gaggaagcct tctcgctggc ccgcgacgcc gccgagcggc gggcgctgtt cgataacctc
      541 gtcgcggcgg tagacccaac cgccaatcat ccgatcacga tcatcgtcgt cctgcgggcc
      601 gacttctacg cccgtttcgc cgagcacgac ggcctgcgcg aactgatcgc tcgccgccag
      661 gagtacatcg gcgcgatgag ccgcgaggaa ctgttccggg ccatcgtcgc cccggcggcg
      721 cgcggcgatt ggaagctgca agaggggttg gtcgagttga tgctcgacga cgccggcgac
      781 gagccgggcg cgctgccgct gctctcccac gccctgctgg cgacctggga acgccgccgc
      841 ggccggacga tgaccctgtc ggcctataag gagtcgggcg gcgtgcgcgg ggccatcgcc
      901 aagacagccg agtctgtttt ccagcagcgc ctgacgccag cgcagcgccc tatcgcccgg
      961 gccatcttcg tgcgcctgac cgaactgggc gacagcgccg acgccgacac ccccgacacg
     1021 cgccgccgcg cttccttcag cgaactcatc acccgcgcca ctgacccgca gatgctcgac
     1081 gccgttctca atatcctcgt cgatgcccgg ctggtcatca ccgacgtggt tccgccgacg
     1141 aacacgcaga tcgtcgaggt ggcccacgag gcgctcatcc gcgagtggcc gacattgcgc
     1201 gcctggctcg accaggatcg cgagggcctg attcgccagc gccagctaac cctggatgtc
     1261 agcgactggc tcaagctggg ccgcgacccc ggcgcgctct accgcggcgc gcggctggcc
     1321 cggacgctgg aatggacggc cgccgcgccc gacgccctca gcgtcgatga agccgaattc
     1381 ctggacgcca gccgggccgc cgccgcccgc gaggcccgcg cccgcacgat gcagcgcgcc
     1441 ctggcgatca gttccgtcgt gctgctggtg ctggccatcg tggcggcgct gctggccttc
     1501 gatgtgatct cgctgcggcc ggcggatcag atgagcggcg actttaacat cgctgtggcc
     1561 gaattcgccg tgctaaacga atccggcgaa ctggtgaagg gcgatggcgg ccggcgcgtg
     1621 gccgagcggc tgggcgcgac gctccagtcg gagttcgccc agcggccgga ggtgcaggtc
     1681 tggttcgaca gcccgacgct ggagcaggag cacaacgtta ccatcggcgt tgtcggtctg
     1741 ccgccgctgc gggccacgcc cccggccgag gcctcggcgg cgctgaacgc cgatgtggtc
     1801 atccacggcg acatccggcc ggcgggcaac ggcaacgcgc tgacactgcg cttctatctg
     1861 cggccccaat tcgaggctga cttcagccag atggttggct tccacgagtt cacggcgcgc
     1921 gtgcccgtct tcgatcccgc caacccgcgc gaggaggtct ggcgcgagct agacccgctg
     1981 tccggcgcgc tggcctggct gatccagggc ctgcggcagg agattctggg cgaccaggcc
     2041 gcggcgctgg cggcgtttga gcaggccgcc acgttagcgc ccgattccga cgtcgtgcag
     2101 tacttcctgg gccaggagca cttctacagc gcccagcggg ccggcggcga cgagacgcaa
     2161 ttggccgcgg ccgagacggc ctttaacgag tccctgcggc tgaaccctga caacgcccgc
     2221 gccagcatcg gtctgggcag cgtccacttc gcgcgcggcc aacggctgtt gaatgaggcc
     2281 gaggcgcgcg gcgagggcgg cgacgcgacg gcgctggcag cggtgcgcgc ggaagcggag
     2341 gcggcgttgg cggcttatac cgccgtggcc ttgcgtggcg agcagatcga gacctatggc
     2401 gtgcccgtgg ccggcatcgc ccgcgtcggg cgggccatcg ccctgcggct gctgggcgag
     2461 acagccttcc agggcggcga cccggccgcg gccgaggccc acatcgatga ggccatcgcc
     2521 gcgctggagg ccgacgccgg ccaactggat attgacaacg acccccggtt ggcggcgcag
     2581 tctgatcagg ctctgggcag tatctatgaa tggaaggcct tcctgctggg cgagcgcggc
     2641 gcggccgacg aggcccaggc ggcgcgagac aaggcgctcg gcttctacaa cgcctgtgtt
     2701 cggcaggggg aggacttccc ctttgacgtc tatctggtgg agcacattgt tggccagctt
     2761 tgcgcgccgc gggtaacggc gttgcaagcg ccgggaggag agggaggatg atggcacgag
     2821 cactgggtgc gggaatcgtg ttgctggtgg tgctggcgct ggctggctgt ggtgggggcg
     2881 aggccgagcc gactgtcctg ccgttgtcca cggccgacaa caatggcgca atcgccacct
     2941 cggtgcagca gaccatcgaa gccattgccg ttgctcagac tgtagccgcc atgggcgtgg
     3001 gccagccgac gccctcgccg gcgagcgagg tcgtggccac ggccgcgatc gtcgagccgt
     3061 cgccgacagc cgcgccgctg cccaccgccg ccgaggctcc ggcgggcacg tgccaggtcg
     3121 tcagcggcgt caatctgcgc cccggccccg gcgtggccta tgacccgcct ctcggcaacc
     3181 tgagcgtcaa cacgcaactg acgccgctgg cctaccaggc cgtcggcttc ccgcaagggc
     3241 cgtggctcca ggcgcaggtg caggccaccg gccaggtcgg ctggatcagc gctctgccac
     3301 agtacgtcca gtgcgacttt gatctggcca cgctgccccc ggccgtcttc ccacccacgc
     3361 cgccgccgcc caccgcgccg ccgccgccca tcgccccgac gaccgtcgcg gcgacggcca
     3421 cgcgcccgct ggcctcagcc ccgcccgaca tcgacaacga cgcccctggc ggcagcttcc
     3481 ccagcaccgg cgtcaccggc aacgtcattg tcgatccggc ttttctcttc cgcatggagg
     3541 tcttcgacga ggccgcgggc aaccacgacg gcgcgggcat ccagttcgtc ttcttcgata
     3601 tttccggcaa tggcattagc tactcgcgcc aggagaacaa tgccggctat tgcgtcttcg
     3661 gcggtggtga gcctacctgt aacccgtggc caaccgacga ccagggacgc tacatctggg
     3721 gtgagggtgg cgacatagtc aagacgggcg actacttcgc ctcgattacc gtcacggctg
     3781 agaaccccga cccagagttc aacggcgtgt ggaattggaa cttctcattc cacgtgactg
     3841 tgccgtgatg tgccggaggg caaacgttag acgatgaaca agatgggccg catcaccgtt
     3901 gggctgttgg cgctgattgt gctggcgacg gcagtggcgg cctgcggcca ggcggccgcg
     3961 ccggcgccca cgaccacgcc gccagaggct atcgcggccg gccccgcttc tgaccgccca
     4021 ccggacgtgg acaacgacgc cccggccggc gagtgtcagg acacgaccaa catcctcagc
     4081 cgcgtcgacc gcgcgcccaa cgcgctcttt cgcgccgtgg ccctcgacaa ccgcgttggc
     4141 ggcaacgacg gcgacggcat cgtcggcgtg cgcttcaccg tgacgggcga caacctctct
     4201 ttcgttaagg acgaactgac cgcgccctat tgcgccttcg gcggcaacga gccggactgt
     4261 ggcgtgtggc cgcgcgacga agccgggcgc tacacctggg gcgcgggcgg gtcgctggtg
     4321 cagccgggac gctttcacgt catggtcgag gtcgtcgcgg ggcagcccga ctccgctacc
     4381 ggccgcgccg gctgcgactg ggaattcgac atgcaggttt ccatgcccta gcccctggc
//
LOCUS       DAOSMQ010000255         4427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19771_length_4427_cov_5.246569, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000255 DAOSMQ010000000
VERSION     DAOSMQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19771_length_4427_cov_5.246569"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PK829_09255"
     CDS             complement(<1..175)
                     /locus_tag="PK829_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOU41443.1"
                     /translation="MTIDQAKARALENTVATLTKRFGDGVIMRLGDAQHMQVESIPTG
                     SISLDIALGVGGVP"
     gene            262..1155
                     /locus_tag="PK829_09260"
     CDS             262..1155
                     /locus_tag="PK829_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU41444.1"
                     /translation="MQLANSSPARPLVDPHVRPATRADAGAIQRLLRLGAYIHLHVDW
                     HVPGDWLGTPGFVVYARDASADADTDTIVACLAVGADPLPAAWVRVAATRTVAAYDQC
                     AAMWAAVLAGLEPEITEVAWFITDNWPLRWLDRLGFTLVSTVLGFRKSDLEPVSVSAP
                     RGLDIRPALVEELPALAEMEAAAFEPRWRHSAQSLYLAWRQSLSFDVATLDGRPVGFQ
                     FSTGGNGNAHLARMTVDPQWQGLGIGGALMAQALEGYRQRKVRTVTLNTQEDNLVSQR
                     LYGRFGFRPTGQKYPVWSYYP"
     gene            1203..1499
                     /locus_tag="PK829_09265"
     CDS             1203..1499
                     /locus_tag="PK829_09265"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015079161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOU41445.1"
                     /translation="MERKKLSETEIQAALPELPGWEVKDGKLHKAYKFESFARAIGWM
                     VSVAVHADKMDHHPEWQNVYNRVTVDLATHDMDDAISTFDVELARKMESLADKG"
     gene            1507..2508
                     /locus_tag="PK829_09270"
     CDS             1507..2508
                     /locus_tag="PK829_09270"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOU41446.1"
                     /translation="MAVNLRNRSFLKELDFTRDELRFLLKLAADLKAAKYGGYEQPRL
                     VGKNIALIFEKASTRTRCAFEVAAYDQGAHVTYLGPEGSQIGHKESMKDTARVLGRMY
                     DGIEYRGFAQETVETLARYAGVPVWNGLTNEFHPTQILADMLTMLEHSHQQPAQIAYC
                     YMGDARFNMGNSLMVGGCKLGMDVRICAPRHLWPSEELIAAGRAAAEETGARLTLTED
                     VDEGVRGVDFIHTDVWVSMGEPDSVWAERIDLLKPYQVNAEAMRRSGNPAVKFMHCLP
                     AFHNRETKVGEGVYQKFGLDGLEVTEDVFESPASIVFDQAENRMHTIKAVMVATLGM"
     gene            complement(2535..3371)
                     /locus_tag="PK829_09275"
     CDS             complement(2535..3371)
                     /locus_tag="PK829_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU41447.1"
                     /translation="MSEEQERPAWSEQFLPEAIRRLNLSDALSALTPEVAWGGSTGRG
                     VRVAVVDTGVEADHPVFEGRVSGGVVIELNDQVEDGFVAIPDNPPRDVAGHGTACAGI
                     IHRLAPEADLFSVRVLGDNMRGRALTFAAGIEWAIGQGFQVVNMSLSTSSADLQKLFY
                     ELADEAYFKNVCLVCAVNNIPAPSYPSTFASVISVAAHHSRDPWTYYYNPSPPVEFGA
                     AGIDIEVAWINKQYATNTGNSFAAPHIAGIVALIRAKHPQLTPFQVKTMLMACAKNVQ
                     AL"
     gene            complement(3386..4192)
                     /locus_tag="PK829_09280"
     CDS             complement(3386..4192)
                     /locus_tag="PK829_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU41448.1"
                     /translation="MTPAAADANAPEEPLAQQLQALIRAIELGGKAILPTSNDELLQS
                     IVNAAARIFGAGAAGILLVDESDGTLVFRVEFGNKQPDLVGRRVPLNKGIAGYVAMTG
                     QAIAVSNVQQDARFNADFAKSTGYVPNSILAAPLLLGERVIGVLEVLDKIAANSFGMH
                     DMELMGLFAHQAALAIRESQQLEQIDSAIRQGLRRIAAAEGLPDSSALVAMLADTHDE
                     ATGPDDLLPLAAAFNDISRLGPAERRAAVKILAALAEVGRSRARPRSRYA"
BASE COUNT          750 a         1359 c         1521 g          797 t
ORIGIN      
        1 ggggcacgcc gcccacgccc agggcgatgt cgagcgagat cgacccggtg gggatcgact
       61 cgacctgcat gtgctgcgcg tcgcccaggc gcatgatgac gccgtcgccg aagcgcttgg
      121 tcagcgtggc gaccgtgttt tcaagtgcgc gtgccttagc ctgatcgatt gtcatgttct
      181 ctggggtcgc cgggataagt cgtatcgggt acatctgccc gggcgatccc ccttcttaat
      241 tatgtagtgt acaatagcgc catgcaattg gcaaactcat cgccggccag acctcttgtc
      301 gatccacatg ttcgcccggc cacgcgcgcc gacgcggggg ccatccagcg gctgttacgg
      361 ctaggcgcat atattcacct ccacgtcgat tggcacgtgc ccggcgattg gctgggcacg
      421 cccggctttg tcgtctacgc gcgcgacgcc agcgccgacg ccgacactga caccatcgtc
      481 gcctgcctcg ctgtgggggc ggaccctctg ccggccgcct gggtgcgcgt ggccgccacg
      541 cggacggtgg cggcctatga tcagtgcgcg gccatgtggg cggctgtcct ggccggtctg
      601 gagccggaga tcaccgaagt ggcttggttc atcaccgaca actggccgct acgctggctc
      661 gaccggctcg gctttaccct cgtcagcacc gtgctcggct tccgcaagag cgacctggag
      721 ccggtatctg tctcggctcc gcgcgggctg gacatccggc cggcgctggt ggaggaactg
      781 ccggcgctgg cggagatgga ggcggccgcc ttcgagccgc gctggcggca cagcgcccag
      841 agcctctatc tggcctggcg gcagtcgctc agcttcgacg tggccactct cgacgggcgg
      901 ccggtcggct ttcagttcag caccggcggc aacggcaacg ctcatctggc gcggatgacc
      961 gtcgatccac agtggcaggg gctgggtatc ggcggagcgc tgatggcgca ggcgctagag
     1021 ggctaccgtc agcgcaaggt gcgcaccgtg acgctgaaca cgcaagagga taacctcgtg
     1081 tcgcagcggc tctatggccg ctttggcttc cggccgacgg ggcagaaata tccggtctgg
     1141 tcatattacc cgtagcccgg cgctacacgg gcacgggcag agaaggagtg gcgtgagata
     1201 agatggagcg gaagaaactg agcgagacgg aaattcaggc ggcgttgcca gagcttcccg
     1261 gctgggaggt gaaggatggc aagctgcaca aggcctataa gttcgagtcg ttcgcccggg
     1321 cgattggctg gatggtcagc gtggctgttc atgctgacaa gatggatcac catccggagt
     1381 ggcaaaacgt ctacaaccgc gtgaccgtag acctggccac ccacgacatg gacgacgcga
     1441 tcagtacttt tgatgttgag ctggcccgca aaatggagtc gctggccgac aaaggataga
     1501 gtttgcatgg cagtcaatct acgcaaccgc agttttttga aggaacttga cttcacccgc
     1561 gacgagctgc gcttcctgct gaagctcgcg gcagacctga aggcggccaa gtacggcggt
     1621 tatgagcagc cgcgcctggt gggcaaaaac attgccctca tctttgagaa ggcctccacg
     1681 cgcacgcgct gcgcctttga ggtggcggcc tatgaccagg gcgcgcacgt gacctacctc
     1741 ggcccggaag gctcgcagat tggccacaag gagtcgatga aggacactgc ccgcgtgctc
     1801 ggccggatgt acgacggcat cgaataccgc ggctttgccc aggagacggt cgagacgctg
     1861 gcgcgctatg ccggcgtgcc cgtctggaac gggctgacca acgagttcca cccgacacaa
     1921 atcctggccg acatgctgac gatgctggag cattcgcacc agcagccggc ccagatcgcc
     1981 tactgctata tgggcgacgc gcgcttcaac atgggcaact cgctcatggt cggcggctgt
     2041 aagctaggca tggacgtgcg catctgcgcg ccccggcacc tctggccgag cgaggaactt
     2101 atcgccgctg gtcgggccgc ggccgaggaa accggcgcgc ggctgacgct gaccgaggat
     2161 gtcgatgagg gcgtgcgcgg cgtggatttc atccacaccg acgtgtgggt gtcgatgggt
     2221 gagccggact cggtctgggc ggagcgcatt gacttgctga agccctatca ggttaacgct
     2281 gaggccatgc gccgcagtgg caacccggcg gtcaagttca tgcactgcct gcccgcgttc
     2341 cacaaccgcg agacgaaggt cggcgagggc gtctatcaga aatttgggct ggacgggctg
     2401 gaagtgaccg aggatgtctt cgagtcgccg gcgtcgattg tcttcgacca ggccgagaac
     2461 cgcatgcaca ccatcaaggc ggtgatggtg gcgacattgg gaatgtagct tgtagggcgg
     2521 gttgccaact cgccctacaa ggcttgcacg ttcttggcgc aggccatgag catggtcttg
     2581 acctgaaacg gcgtcaactg gggatgcttg gcgcggatca gggcgacgat gccggcgatg
     2641 tgcggcgcgg cgaagctgtt gcccgtattg gtcgcgtatt gcttgttgat ccaggccacc
     2701 tcgatatcga tgccggccgc gccgaattcg accggtgggg acgggttgta gtagtacgtc
     2761 cacgggtcgc gcgagtggtg ggcggctacg gagatgaccg aggcgaacgt cgatgggtag
     2821 ctgggcgcgg ggatgttgtt gacggcgcag accaggcaga cgttcttgaa gtaggcctca
     2881 tcggctagtt cgtagaacag cttttgcaag tcggcgctgg acgtgctcag gctcatgttg
     2941 acgacctgga agccctggcc gatggcccac tcgatgccgg cagcgaaggt cagggcgcgg
     3001 ccgcgcatgt tgtcgcccag aacgcgcacg ctgaagaggt ctgcctctgg ggccaggcga
     3061 tggatgatgc cagcgcaggc ggtgccgtgg ccggccacgt cgcggggcgg attgtcgggg
     3121 atggccacaa agccatcctc gacctgatcg tttagctcga tgacgacccc gccgctgacg
     3181 cgcccctcga agaccggatg atcggcctcc acgcccgtat cgaccacagc gacgcgcacc
     3241 ccccggccgg tgctgccgcc ccaggcgact tcgggcgtga gcgcgcttag ggcgtcgctg
     3301 aggttcaggc ggcggatggc ttccggcagg aattgctccg accaggccgg gcgctcttgt
     3361 tcttcgctca tggccacctg agtgcttagg cgtagcgcga gcgcggcctg gcgcgcgagc
     3421 ggcccacttc ggccagggcg gccaggattt ttaccgcggc gcggcgctcg gctgggccta
     3481 accggctgat gtcgttgaag gctgcggcca gaggcagcaa gtcatctggc cccgtagcct
     3541 catcatgagt gtccgccagc atcgctacca gcgccgacga atcgggcagc ccctccgcgg
     3601 cggcgatgcg gcgtagacct tgccggatgg cgctatcgat ctgctccagc tgctgcgact
     3661 cgcggatggc cagagcggcc tgatgggcaa agaggcccat cagttccatg tcgtgcatcc
     3721 caaaggagtt agcggcgatc ttgtccagca cttccagaac gccaatgacc cgttcgccca
     3781 gcagcagcgg ggcggcgagg atggagttgg gcacgtagcc ggtgctcttg gcaaagtcgg
     3841 cgttgaagcg cgcatcctgc tggacgttgg acacggcgat ggcctggccg gtcatggcca
     3901 cgtagccggc aatgcccttg ttgagcggca cgcgccggcc gacgaggtcg ggctgcttgt
     3961 tgccgaactc cactcggaag acgagcgtgc cgtcactctc atcaaccagc aggatgccgg
     4021 ccgcgcccgc gccgaagata cgcgcggcgg cgttgacgat cgattgcagc aactcatcgt
     4081 tggatgtggg caggatggcc ttaccgccca gttcgatggc gcggataagg gcctgcaatt
     4141 gttgggcgag cggctcctcc ggcgcgttgg cgtctgctgc ggcgggcgtc atatggctct
     4201 cctcgtctcc agcccgttac cgggcctcct gaccatgcgg tcgcgcccgg cctttggacg
     4261 cgctacagcg gccggccgct ccgttggcca caaggcaacg gtcggccggc caccagtcgt
     4321 ttccggcgga tacccttttt actcgacgtc gtaagcctcg tcgtcggggt tgtagacgac
     4381 cctaaaggtg acgcggccgg ccgaggcttt ggcggtcatg ccgtggg
//
LOCUS       DAOSMQ010000256         4424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19786_length_4424_cov_4.532387, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000256 DAOSMQ010000000
VERSION     DAOSMQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19786_length_4424_cov_4.532387"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PK829_09285"
     CDS             complement(<1..320)
                     /locus_tag="PK829_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007460918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU41449.1"
                     /translation="MNVLTRCRWRAIVLLVVLCLALAGCGGAEIVAPAAVAATQPPVA
                     EQPAEAETTLTVFAAASLTDAFGEVATAFSAANPGVTVAFNFAGSNQLATQIGAGAPA
                     DVFA"
     gene            complement(317..904)
                     /locus_tag="PK829_09290"
     CDS             complement(317..904)
                     /locus_tag="PK829_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOU41450.1"
                     /translation="MNTHSLSNGPAEPASLELALIGFVEDAPRHGYEIHQLLTDTPEL
                     RRIWRMKQSRLYAMLARLEAAGLLRSDVVTQAGRPPRKLLRPTEAGASAFGRWRTEPV
                     AQPREMRLEFMLKLYFAVRAGPEAARQLVSAQEAVCAGWPEMRPDPADGPFLHAVVAY
                     RRAHIAAIRGWLASLATATMPLTESPTPYEDRSPS"
     assembly_gap    1066..1165
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1376..2080
                     /locus_tag="PK829_09295"
     CDS             1376..2080
                     /locus_tag="PK829_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOU41451.1"
                     /translation="MALLALLVALVACAGAGGAPLTLPATRRPPTATPPGLTPRPQTP
                     APRGTAVPEGDRATVTYVIDGDTIEVEQGGRTYRLRYIGVDTPEREEPYYQEARDFNR
                     DLVADQTVILVRDVSETDQYGRLLRYVYLEDGTFVNAAIIAAGMARLVTFPPDVAQTE
                     FLKGLQAEAREAGRGMWGDAATGPCDCDRNLYNCRDFDTQAEAQTCFEYCLDTAGRDV
                     HNLDGGGDGLVCESLP"
     gene            2085..2591
                     /locus_tag="PK829_09300"
     CDS             2085..2591
                     /locus_tag="PK829_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU41452.1"
                     /translation="MLLYLFGLPAAGKNYVGQVLADTFGYTFYDGDLDLTEELRDAVR
                     QERPFTDTMRDRFYAVLIERIAALRREYTDLAFGQATFKERHRALVAEAFPDVVFVLV
                     EAAEEVRLARLARGGNPVTVDYARQIAAFFEPPRHAHFVIRNEGGRAEIVAQLERLLA
                     TLAVAGYV"
     gene            complement(2588..3073)
                     /locus_tag="PK829_09305"
     CDS             complement(2588..3073)
                     /locus_tag="PK829_09305"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOU41453.1"
                     /translation="MSEAQTPVGRALAATGIPFHEFQHPGPVLSLEQAAQERNQRPEQ
                     VVRSILFRLGADEYALVLVAGPRQVDWKALRRYLGRSRLTTAPAEEVYAITGYEIGAV
                     SPFGLPRPMRVLLDESVAREAEVSIGSGVRGTTVILTTADLLRALGGGDPVTVERVSL
                     T"
     gene            complement(3066..3815)
                     /locus_tag="PK829_09310"
     CDS             complement(3066..3815)
                     /locus_tag="PK829_09310"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOU41454.1"
                     /translation="MAGEASRARPLIGLTTYRKTAAQTNPVALYALMPSYVEAVIAAG
                     GVPVMIPLGLDEISLRTLLARLDGLLLTGGGDIAGDHYGSANPGLIFDVDADRDRVEL
                     FLAREAVDSDKPLLAICRGHQILNVALGGSLYEDVLRLMPSAIKHDYWGEYPRTFLSH
                     EVIIDEGTTLARRLGETCVRVNSLHHQGLRDLAPDLVAVAHAPDGLVEAVEAPGRRYA
                     IGVQWHPENLIHVAPPMLSLFRGLVEAAAGE"
     gene            complement(3831..4196)
                     /gene="rplS"
                     /locus_tag="PK829_09315"
     CDS             complement(3831..4196)
                     /gene="rplS"
                     /locus_tag="PK829_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOU41455.1"
                     /translation="MSDLLLKAFDNEKAANMPTVQIGDNVTVYVRINVGDKNERVQMV
                     RGTIIRVRNSGNNANFTVRRIASNGIGVERTFLASSPRLEKIEIHSHSHIRRARLYYL
                     RERTGKATRLRSKRAFDQK"
BASE COUNT          721 a         1434 c         1448 g          721 t
ORIGIN      
        1 ctggcgaaga cgtcggctgg cgcgcccgcg ccgatctgcg tcgccagctg gttggagccg
       61 gcgaagttga aggccacggt cacgccgggg ttggcggcgc tgaaggccgt agcgacctcg
      121 ccaaaggcgt cggtcagcga ggcggcggca aacacggtca gcgtcgtttc ggcctcggcc
      181 ggttgctcgg ccacgggcgg ttgggtggcc gcaaccgccg ccggcgcgac tatctctgct
      241 ccgccacagc cagcgagcgc caggcagaga accacaagca agacaatggc ccgccagcgg
      301 caacgggtga gcacgttcat gatggactcc tgtcttcgta aggtgttgga ctctcggtca
      361 aaggcatcgt cgcggtggcc agcgacgcca accacccgcg gatggcggcg atatgggcgc
      421 ggcgataggc cacgacggca tggaggaacg gcccgtcggc cgggtcgggc cgcatctccg
      481 gccacccggc acagacggcc tcctgggcgc tcaccagttg ccgcgccgct tccgggccgg
      541 cgcgcacggc aaagtagagc ttgagcatga attccaggcg catctcccgc ggctgggcta
      601 ccggctctgt ccgccagcgg ccaaaggcag acgcgccggc ctccgtcggc cggaggagct
      661 tgcgcggcgg ccggccggcc tgcgtgacca cgtctgagcg cagcagcccc gcggcctcca
      721 gccgggccag catggcgtag aggcggctct gcttcatgcg ccagatgcgg cgcaactccg
      781 gcgtgtcggt caacagttgg tggatttcgt agccgtggcg gggcgcgtcc tcgacaaagc
      841 cgataagggc cagttccagc gaggccggct ccgcagggcc attactcaaa gagtgagtat
      901 tcataaggtg agtataaccc tgccagtgaa tgtcgcaacc tgtagcgccc gcagcgcccc
      961 ctggaggctg tggcgcctgt ggcgccattc tccgaatcgc cctcttgcgc ggcgcgccct
     1021 cttgcgtggc gcgattctcc gaatcgccct cttgcgtggc gcgccnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnntcttg cgtggcgcga ttctccgaat cgccctcttg
     1201 cgtggcgcgc cctcttgcgt caaccagcat aagaaagcga ttcagagaat cgcgccacac
     1261 ggcggtcgac ggctgtcccg aaagcgagta ccatcccgcc aataagccct accattacga
     1321 cgacgcgccc ctgacacctg tacccatgcc attcacctac cgacgccccc tccttatggc
     1381 cctgcttgcc ctgctggtgg ctttggtggc ctgtgcgggc gcgggcggcg cgccgctcac
     1441 gttgccggcc acacgacgcc cacccacagc gacgccgccc ggcctgaccc cacgccccca
     1501 gacgccggcg cctcgtggca cagccgtgcc ggagggcgac cgcgccacag taacctatgt
     1561 cattgacggc gacaccatcg aggtggaaca gggcggtcgc acctaccgtc tgcgctacat
     1621 cggcgtcgat acgccggaac gcgaggagcc gtactatcag gaggcgcgcg acttcaaccg
     1681 cgacctggtg gcagaccaga ccgttatcct cgtgcgcgat gtcagtgaga ccgatcagta
     1741 cggccgtctg ctgcgttatg tctatctgga ggatggcacg tttgtcaacg cggcgatcat
     1801 cgccgccggc atggcccggc tggtgacctt tccgcccgat gtggcccaga cagaattcct
     1861 gaagggcttg caggccgaag cccgtgaggc cggacggggg atgtggggcg acgcagccac
     1921 cgggccatgc gattgtgacc gcaacctcta caactgccgc gacttcgaca cgcaggccga
     1981 ggcgcaaacc tgcttcgagt actgcctcga tactgccggg cgcgatgtcc acaatctcga
     2041 cggtggcggc gatggcctcg tgtgcgagtc cttgccctag cgccatgctg ctctacctct
     2101 tcggcctccc ggcggcgggc aagaattacg tgggccaggt gttggccgat acattcggct
     2161 acaccttcta cgatggcgac ctcgacctga ctgaggaact gcgcgacgcc gtgcgccagg
     2221 agcggccgtt caccgacacc atgcgcgacc gcttctatgc cgtccttatc gagcgcatcg
     2281 ccgccctgcg gcgcgaatac accgacctgg ctttcggcca ggcgacgttc aaggagcgcc
     2341 atcgcgcgtt ggtggctgag gctttcccgg acgtggtctt tgtgctggtg gaggcggctg
     2401 aagaggtgcg cctggcgcgg ctggcacggg gcggcaaccc ggtgaccgtc gattacgccc
     2461 ggcagatcgc cgccttcttc gagccacctc gccacgccca cttcgtcatt cgcaacgagg
     2521 gcgggcgcgc ggagatcgta gcccagttgg aacgcttgct ggccaccctg gctgtcgctg
     2581 gctacgtcta ggtcaagctg acgcgctcaa cggtgaccgg gtcgccaccg ccgagggcgc
     2641 gcaacaggtc ggctgtggtt aggatgaccg ttgtcccacg cacgccggag ccgatggaca
     2701 cctcggcctc gcgggcgacg ctctcatcca acagcacgcg catcggccgg ggcaggccaa
     2761 aaggggacac cgcgccgatc tcgtagccgg tgatggcgta gacttcctcg gccggggcgg
     2821 tggtcaggcg cgagcggccc agatagcgac gcagcgcttt ccagtccacc tgccgcggcc
     2881 cagccaccag caccagcgca tactcgtccg cgcccaggcg gaagaggatg ctgcggacga
     2941 cctgctccgg gcgttggttg cgctcctggg cggcctgttc cagcgagaga acggggccgg
     3001 ggtgttggaa ttcgtgaaag gggatgccag ttgccgccag ggcgcggccg acgggcgtct
     3061 gcgcctcact caccggcggc ggcctcgacc agcccccgga agagggacag catcggcggg
     3121 gcgacgtgga tgaggttctc cgggtgccac tgcacgccaa tggcatagcg ccgccccggc
     3181 gcttcgacgg cctccactag cccatcgggc gcgtgggcca cggcgaccag gtcgggggcc
     3241 aggtcgcgca ggccctgatg gtggaggcta ttgacacgca cgcacgtctc acccagccgg
     3301 cgggccaggg tcgtgccttc gtcgatgatc acctcgtggg acagaaaggt gcgtgggtat
     3361 tcaccccagt agtcgtgctt gatggctgag ggcatcagcc gcaggacatc ctcatagagc
     3421 gagccgccca gggccacatt gaggatctga tggccgcggc agatggccag cagcggcttg
     3481 tcactgtcca cggcctcgcg agccaggaac aactcgacgc gatcgcggtc ggcgtcaacg
     3541 tcgaagatca ggccggggtt ggcgctgccg tagtggtcgc cggcaatgtc gccgccacca
     3601 gttagcagca ggccatccag ccgggccaac agcgtccgca gactgatctc gtccaggccc
     3661 agtgggatca tcaccggcac gccgccggcg gcgataaccg cctcgacgta ggagggcatg
     3721 agagcataga gcgcgacggg gtttgtctgc gccgcggtct tgcgataggt cgtcaggcca
     3781 atgagcggcc gcgcccgcga agcttcgcca gccatggaca ggggatgcga ttatttctgg
     3841 tcgaaggcgc gcttgctgcg caggcgcgtg gccttgccgg tgcgctcgcg cagatagtag
     3901 aggcgggcgc ggcggatgtg gctgtggctg tggatctcga tcttttccag gcgcggcgag
     3961 ctggccagga aggtgcgctc gacaccaata ccattggagg caatgcggcg cacggtgaaa
     4021 ttggcattgt tgccggagtt gcgcacgcgg atgatggtgc cacgcaccat ctggacgcgc
     4081 tcgttcttgt cgcccacatt gatgcgcacg taaacggtga cattatcgcc gatctgcacc
     4141 gtgggcatgt tagccgcctt ctcgttgtca aacgctttca gtagtaagtc agacatctca
     4201 cacttctcca atggctggaa aaaacaacgg cccgactggg accgtctcct gcccccggca
     4261 aaccgggcat aatgattgcg tagttaagac ccgcagggcg ggccgattga ggactatacc
     4321 acgccgtggc tgctttggca acggccattt gccgttttgc ggggttcttc agtcgtcggt
     4381 cttcgcgcgg ctcaacgcac agcccgtagc gtagcacagc ctgt
//
LOCUS       DAOSMQ010000257         4418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19828_length_4418_cov_3.995416, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000257 DAOSMQ010000000
VERSION     DAOSMQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19828_length_4418_cov_3.995416"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PK829_09320"
     CDS             <1..918
                     /locus_tag="PK829_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOU41456.1"
                     /translation="SQSRPLSSVNRRETIRNYVYLTAGALIMMVNFNIFMAPSNIAPG
                     GVGGLSLILNHYTGIPRGAGMLLLSIPLIALGFWKLGRFRFLVRTLYVTVFYTIGVDV
                     TARLLPAGGLVDDLLLTAVFGGVLGGIGYGLVLRGRGMVSGTGIISRVLQLRTGIPVS
                     QLYMVIDGVVIVALGLTFGWERALYGMIMLFIMGLASDYVLEGPSVVRVVFIVTDLPE
                     AVAAVLLERLRVGVTRWTGEGMYTAEERAILFCTVTRPDVELLHSVVTEIDPAAFVVI
                     GQGHQATGGIVRRPDLAKKAATEATADVA"
     gene            1511..2437
                     /locus_tag="PK829_09325"
     CDS             1511..2437
                     /locus_tag="PK829_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU41457.1"
                     /translation="MAHYDDRPADATDGVSRATGANDNGSGVALLLETARIMSSREWN
                     QTVVFLATSAEEQGSFGARYFAENAFLDNMNIIAALNYDAVGGRAGIPQHARLFAVQY
                     AISRHGALGRYYEYVAGLYLPTFPVLIQDALDRDGRWGDHREFVAVGFPAIRVIESVE
                     DPELLNSNRDTWDLIDYNYLQRIVQINLAVAANLAGGPQQPQPPLVATMAEPGAYMLT
                     WPVDPVAAGYAISFRPEGNGIAGRDRVHRPGQHVRARLGHRGHEGRLRLLRPPGQVGG
                     HGQIDLDDPLKIVVVDQVPRVAVGIQQLRVFN"
     gene            complement(2402..2908)
                     /locus_tag="PK829_09330"
                     /pseudo
     CDS             complement(2402..2908)
                     /locus_tag="PK829_09330"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(3501..>4418)
                     /locus_tag="PK829_09335"
     CDS             complement(3501..>4418)
                     /locus_tag="PK829_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOU41458.1"
                     /translation="SQSRPLSSVNRRETIRNYVYLTAGALIMMVNFNIFMAPSNIAPG
                     GVGGLSLILNHYTGIPRGAGMLLLSIPLIALGFWKLGRFRFLVRTLYVTVFYTIGVDV
                     TARLLPAGGLVDDLLLTAVFGGVLGGIGYGLVLRGRGMVSGTGIISRVLQLRTGIPVS
                     QLYMVIDGVVIVALGLTFGWERALYGMIMLFIMGLASDYVLEGPSVVRVVFIVTDLPE
                     AVAAVLLERLRVGVTRWTGEGMYTAEERAILFCTVTRPDVELLHSVVTEIDPAAFVVI
                     GQGHQATGGIVRRPDLAKKAATEATADVA"
BASE COUNT          762 a         1447 c         1447 g          762 t
ORIGIN      
        1 agccaatccc gaccgttgtc gtcggtcaac cggcgcgaga ccatccgcaa ctacgtctac
       61 ctgacggccg gcgcgttgat catgatggtc aacttcaaca ttttcatggc ccccagcaac
      121 atcgcgccgg gcggcgtggg tggcctgtcg ctcattctga accattacac cggcattccc
      181 cgcggcgcgg gtatgttgct gctcagcatc ccgctgatcg ctctgggctt ctggaagctt
      241 ggccgctttc gcttcctggt gcggacgctc tacgtgaccg tgttctacac cattggcgtc
      301 gacgtgaccg cgcgcctact gccggccggc ggcctggtcg atgacctgct gctgacggcc
      361 gtcttcggcg gcgtcctggg gggcatcggt tacgggttgg tgctgcgcgg ccggggcatg
      421 gtcagcggca cgggcatcat cagccgcgtg ctgcaactgc gcaccggcat ccctgtcagc
      481 caactctaca tggtcatcga cggtgtggtc atcgtcgccc tggggttgac gttcggctgg
      541 gagcgggcgc tctatggcat gatcatgctc ttcatcatgg ggctggcctc ggactatgtg
      601 ctggaagggc caagcgtggt gcgcgtcgtc ttcatcgtca ctgacctgcc agaggcggtg
      661 gccgcagtgc tgctggagcg gttgcgcgtc ggcgtcacgc gctggacggg cgagggcatg
      721 tacacagccg aggagcgggc catcctgttc tgcaccgtca cccgaccaga cgtggagttg
      781 ctacattctg tcgtcaccga gatcgacccg gcggccttcg tcgtcattgg ccagggccac
      841 caggccaccg gcggcattgt gcggcggccc gacctggcca aaaaggccgc gaccgaggcg
      901 acggccgacg tagcttagcc gttgctcagg cgtcgtgcac aggcgggtca tttcggctat
      961 cctttgccgt cgtcaggaat tatcaggaaa atggatttgc cgatttggaa gcatggggtg
     1021 gccagtgtca cgagcgatca tacgcacctt cctcctcctg gtgctggggc tgctgagcct
     1081 ggctggttgt agcctgctgg agccgcagcc acagctcgtc attcccacgc cgccggcggc
     1141 cacgccggcc ctgttcatcc ccgacgcgtc tggacagatc gtcaccgatc cggtcagcga
     1201 cgttgtgccg accatcgacc cgctcatcga ggctctgatt caggagacct cgcagcagca
     1261 gctcatcgcc tatgtccggc aaatggagag cttcggcacg cgcaacgcct tcagcgatcc
     1321 tctgtcagag acattcggca tcggcgcggc gcggcgctgg atcttcaacg agttcttgcg
     1381 cgttggcaac gggcggctac aggtcgagtt ccaggacttc cccatgacct acggcggcta
     1441 ttcggccgac cagagcaacg tcgtggccac gctgccgggc gtgggcggcg gcaacggcgt
     1501 cgtcgtgatc atggcccact acgacgaccg gccagcggac gccaccgacg gcgtcagccg
     1561 ggccacaggc gcaaacgaca acggctccgg cgtggccctg ctgctggaga cggcgcggat
     1621 catgagttca cgcgagtgga accagaccgt cgtcttcctg gcaacctcgg ccgaggagca
     1681 aggctcattc ggcgcgcgct atttcgccga gaatgccttc ctggacaaca tgaacatcat
     1741 cgccgcgctc aactacgacg ctgtaggcgg ccgggccggc attccccagc acgcccgcct
     1801 gttcgccgtc caatacgcca tctcgcgcca cggggcgctg ggccgctact acgaatacgt
     1861 cgccggcctc tacctgccaa ccttcccggt gctcatccag gacgccctcg accgcgatgg
     1921 ccgctggggc gaccaccgcg agttcgtggc cgtcggcttc ccggccatcc gcgtcatcga
     1981 gtcagttgaa gacccggagt tgctgaattc caaccgcgac acgtgggacc tgatcgacta
     2041 caactatctt cagcggatcg tccaaatcaa tctggccgtg gccgccaacc tggccggggg
     2101 gccgcagcag ccgcagccgc ccctcgtggc cacgatggcc gagccgggcg cgtacatgct
     2161 gacctggccg gtggacccgg tcgcggccgg ctatgccatt tccttccggc cggaaggaaa
     2221 tggcatagcc ggccgcgacc gggtccaccg gccaggtcag catgtacgcg cccggctcgg
     2281 ccatcgtggc cacgaggggc ggctgcggct gctgcggccc cccggccagg ttggcggcca
     2341 cggccagatt gatttggacg atccgctgaa gatagttgta gtcgatcagg tcccacgtgt
     2401 cgcggttgga attcagcaac tccgggtctt caactgactc gatgacgcgg atggccggga
     2461 agccgacggc cacgaactcg cggtggtcgc cccagcggcc atcgcggtcg agggcgtcct
     2521 ggatgagcac cgggaaggtt ggcaggtaga ggccggcgac gtattcgtag tagcggccca
     2581 gcgccccgtg gcgcgagatg gcgtattgga cggcgaacag gcgggcgtgc tggggaatgc
     2641 cggcccggcc gcctacagcg tcgtagttga gcgcggcgat gatgttcatg ttgtccagga
     2701 aggcattctc ggcgaaatag cgcgcgccga atgagccttg ctcctcggcc gaggttgcca
     2761 ggaagacgac ggtctggttc cactcgcgtg aactcatgat ccgcgccgtc tccagcagca
     2821 gggccacgcc ggagccgttg tcgtttgcgc ctgtggcccg gctgacgccg tcggtggcgt
     2881 ccgctggccg gtcgtcgtag tgggccatga tcacgacgac gccgttgccg ccgcccacgc
     2941 ccggcagcgt ggccacgacg ttgctctggt cggccgaata gccgccgtag gtcatgggga
     3001 agtcctggaa ctcgacctgt agccgcccgt tgccaacgcg caagaactcg ttgaagatcc
     3061 agcgccgcgc cgcgccgatg ccgaatgtct ctgacagagg atcgctgaag gcgttgcgcg
     3121 tgccgaagct ctccatttgc cggacatagg cgatgagctg ctgctgcgag gtctcctgaa
     3181 tcagagcctc gatgagcggg tcgatggtcg gcacaacgtc gctgaccgga tcggtgacga
     3241 tctgtccaga cgcgtcgggg atgaacaggg ccggcgtggc cgccggcggc gtgggaatga
     3301 cgagctgtgg ctgcggctcc agcaggctac aaccagccag gctcagcagc cccagcacca
     3361 ggaggaggaa ggtgcgtatg atcgctcgtg acactggcca ccccatgctt ccaaatcggc
     3421 aaatccattt tcctgataat tcctgacgac ggcaaaggat agccgaaatg acccgcctgt
     3481 gcacgacgcc tgagcaacgg ctaagctacg tcggccgtcg cctcggtcgc ggcctttttg
     3541 gccaggtcgg gccgccgcac aatgccgccg gtggcctggt ggccctggcc aatgacgacg
     3601 aaggccgccg ggtcgatctc ggtgacgaca gaatgtagca actccacgtc tggtcgggtg
     3661 acggtgcaga acaggatggc ccgctcctcg gctgtgtaca tgccctcgcc cgtccagcgc
     3721 gtgacgccga cgcgcaaccg ctccagcagc actgcggcca ccgcctctgg caggtcagtg
     3781 acgatgaaga cgacgcgcac cacgcttggc ccttccagca catagtccga ggccagcccc
     3841 atgatgaaga gcatgatcat gccatagagc gcccgctccc agccgaacgt caaccccagg
     3901 gcgacgatga ccacaccgtc gatgaccatg tagagttggc tgacagggat gccggtgcgc
     3961 agttgcagca cgcggctgat gatgcccgtg ccgctgacca tgccccggcc gcgcagcacc
     4021 aacccgtaac cgatgccccc caggacgccg ccgaagacgg ccgtcagcag caggtcatcg
     4081 accaggccgc cggccggcag taggcgcgcg gtcacgtcga cgccaatggt gtagaacacg
     4141 gtcacgtaga gcgtccgcac caggaagcga aagcggccaa gcttccagaa gcccagagcg
     4201 atcagcggga tgctgagcag caacataccc gcgccgcggg gaatgccggt gtaatggttc
     4261 agaatgagcg acaggccacc cacgccgccc ggcgcgatgt tgctgggggc catgaaaatg
     4321 ttgaagttga ccatcatgat caacgcgccg gccgtcaggt agacgtagtt gcggatggtc
     4381 tcgcgccggt tgaccgacga caacggtcgg gattggct
//
LOCUS       DAOSMQ010000258         4396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_19940_length_4396_cov_4.576826, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000258 DAOSMQ010000000
VERSION     DAOSMQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19940_length_4396_cov_4.576826"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..442)
                     /locus_tag="PK829_09340"
     CDS             complement(32..442)
                     /locus_tag="PK829_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU41459.1"
                     /translation="MTDSAINDDEYIALAAFRYELRQFLRFSETAAETVGLTPQQHQA
                     LLAIRGFPGPGEMTVGGLAERLQIKPHSAVGLVNRLVAQNLLTRHASTADRRQVFLAL
                     TPPGNALLAELTAAHKAEVRRIRTTLQELLARLD"
     gene            605..2446
                     /locus_tag="PK829_09345"
     CDS             605..2446
                     /locus_tag="PK829_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU41460.1"
                     /translation="MPEKVHFDSATRPTAPSNGSGRLLSLSLLAAIIGVVAGFVAYLL
                     VSLIGLISNLVFFQRVGFDIPDLQGHTLGPLVIVVPVIGGLIIGLMAKYGTPKIRGHG
                     IPEAMEAVLVNRSRISPNVALLKPLSAAIAIGTGGPFGAEGPIIQTGGALGSLVGQFF
                     HVTAAERKVLLSCGAAAGMAATFSTPIAAVILAIELLLFEFKARSFIPLVIASTLATT
                     VHFGLMGRGPMFEVGRLDFGIPTALPFYVILGLLCGVFATLFSNALYQVEDLFERLPL
                     DPMWWPALGGLALGIIGLFVPRVLGVGYANITAILNNEFSVTVLLTIVVFKSLALFLT
                     LGSGTSGGLLAPTFMVGAALGALFALAVNAVLPGANLSPGACALVGMAAVFGAASRAT
                     FTFIIFAFELTRDYNAILPLMLVCVIADLIALRFMRNSIMTEKLARRGLRIQQEYEAD
                     IMQQVSVAEVMDASPPRLPANMLLTELADRIARGDSLVTRRHGAPIVDEEGRLVGIIT
                     QGDVLRALERADSAALTILEAGTPDPLVTYPDELMHDAIGKMLHNDIGRLPVVRRDRP
                     DELVGYLSRAGILAARLMRLKEENELEPGWLLRMRGRTNGTSTGVKP"
     gene            complement(2461..2850)
                     /locus_tag="PK829_09350"
     CDS             complement(2461..2850)
                     /locus_tag="PK829_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU41461.1"
                     /translation="MPPGAIIPELAYTDVAVAAAWLCAAFGFRERLRIGAHRRQLVYG
                     DEAIVVTDGANEAPGGQRLMVRVANADAHCARAVQAGARILTEPTTHPFGERQYTAED
                     IGGHRWTFSQSVADVDPEAWGGVVVEG"
     gene            complement(2881..3171)
                     /locus_tag="PK829_09355"
                     /pseudo
     CDS             complement(2881..3171)
                     /locus_tag="PK829_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
     gene            complement(3239..>4396)
                     /locus_tag="PK829_09360"
     CDS             complement(3239..>4396)
                     /locus_tag="PK829_09360"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009557345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU41462.1"
                     /translation="VDFNVPLSSKDPNANITVTDDTRIRAALPTLNYLLDHGAALILA
                     SHLGRPKTADDRQFSMAPVAKKLEEALGRPVRYVPEVMSDAVKAAAAALQPGDVLLLE
                     NTRFDPREKKNDAQLGADFASLADVYVDDAFGSAHRPDASVDGAAYAMRAKGGPVVAG
                     FLMGQEVSALSIAVDNPPRPYVAIMGGAKISDKIKLIENLLQKADKILIGGGMANTFL
                     KAQGHNLGRSLVEEEALPEARRLLDMAADQLILPVDVVVAAEVSNDAPSEVEMVWAIS
                     PDKMALDIGPATVERFNAELQGAQLVVWNGPMGVFETPQFANGTNQLAQILAGMVDHG
                     AKVIIGGGDSAAAVRKAGLTDKMTHVSTGGGASLELLEGRQLPGVVALDDK"
BASE COUNT          709 a         1439 c         1426 g          822 t
ORIGIN      
        1 cggccaatcc aggagaagac cggtctccgt tctagtctag ccgcgccaac aactcttgca
       61 gcgtcgtccg gatgcggcgc acctccgcct tgtgggccgc ggtcaattcg gccaggagtg
      121 cgttgcccgg cggcgtcagg gccaggaaca cttgccggcg atcggccgtc gatgcgtgcc
      181 gggtgagtag gttctgcgcc actagccggt tcaccagccc cacggcgcta tgaggtttga
      241 tctgcaagcg ctccgccagc ccgccgacag tcatctcgcc aggcccaggg aagccgcgga
      301 tggccagtag cgcctggtgc tgctgtggcg tcagaccaac cgtctcggcc gccgtctcgc
      361 tgaagcgcag gaactgtcgc agttcgtagc ggaacgcggc cagagcgatg tattcgtcgt
      421 cattgatcgc gctgtcggtc atgggaatga ttataccagc agtgccacgg gcgaagaccg
      481 catgcgaagc acgccagggc gtaaggaagg ccacagacga gtcgtaaggc ccattgattt
      541 atatcgcaat acgatataaa atccattact gttcgttctg aacaggctaa agtcggagaa
      601 attcatgccc gaaaaagttc atttcgattc tgcaacgcgg ccaaccgcgc ccagcaacgg
      661 tagtggccgc ctgctgtcgc tgtctcttct ggcggcgatc atcggcgtcg ttgccgggtt
      721 tgtggcttat cttctcgtct cgctcatcgg tctgatcagt aatctcgtct tctttcagcg
      781 ggtcggcttt gacatccccg accttcaggg gcacacgttg gggccgctag tcatcgtcgt
      841 gccggtcatc ggcgggctga tcatcggcct aatggccaag tacggcacgc ccaagatccg
      901 cggccacggt atccccgaag cgatggaggc agtactggtc aaccgcagcc gcatctcgcc
      961 gaacgtggcc ttgctcaaac ccctgtcggc ggccatcgcc atcggtactg gcgggccgtt
     1021 tggcgcggag gggccaatca ttcagaccgg cggcgcgctt ggctcgctgg tgggccagtt
     1081 cttccacgtg acggccgccg agcgcaaggt actattatcc tgtggcgcgg cggcgggcat
     1141 ggccgcgaca ttcagcaccc cgatcgccgc cgttatcctg gcgattgagc ttctgctctt
     1201 tgagttcaag gcccgttcat tcatcccgct cgtgatcgcc agcacactgg cgacgacggt
     1261 tcacttcggc ctgatgggac gcgggccaat gtttgaagtc ggccggctcg actttggcat
     1321 ccccaccgct ctacccttct acgtcattct gggcctgctg tgcggcgtct tcgccacgct
     1381 cttcagcaac gccctctatc aggtggaaga tctgtttgaa aggctgccgc tggacccgat
     1441 gtggtggccg gcgctgggcg gcctggcgtt gggcatcatt ggcctcttcg tgccgcgtgt
     1501 gctcggtgtc ggctatgcca acatcaccgc catcctcaac aacgagttct ccgtcactgt
     1561 cctgctcacc atcgtcgtct tcaagagcct ggccctcttt ctgaccctgg gctccggtac
     1621 gtccggcggt ctactggccc cgacgttcat ggtcggcgcg gcgttggggg cactgttcgc
     1681 tctggcggtc aacgccgtcc ttccgggggc caacctttcg cccggcgcct gtgccctggt
     1741 gggcatggcc gcggtcttcg gcgcagcctc gcgggcaacc ttcaccttta tcatcttcgc
     1801 cttcgagttg acacgcgact acaacgccat cctgccattg atgctcgtct gcgtcatcgc
     1861 cgatctgatc gccctgcgct tcatgcgtaa ctcgatcatg accgaaaagc tggctcgccg
     1921 tggcttgcgt atccagcagg agtatgaggc cgacatcatg cagcaggtgt ctgtggccga
     1981 ggtgatggac gccagcccgc cgcgcctgcc ggcgaacatg ctcctgacgg aactagccga
     2041 tcggattgcg cgtggcgact cgttggtgac aaggcggcac ggcgcaccca tcgtggacga
     2101 ggaaggccgc ctggtgggca tcatcaccca gggggacgtc ctccgtgccc tggaacgcgc
     2161 ggatagcgct gcgctgacta ttctggaggc ggggacgccc gatccgctgg tgacataccc
     2221 cgacgaactg atgcacgacg ccattggcaa gatgctgcac aatgacatcg gccggctgcc
     2281 cgtcgtccgc cgcgaccggc cggacgagtt ggttggctac ctcagccggg ccggcattct
     2341 ggcggcgcgt ctgatgcggc tgaaggaaga gaacgagctg gaaccgggct ggctcttgcg
     2401 catgcgcggc cgaaccaacg gcacgagcac gggcgtcaag ccatagcgcc aacggcaacg
     2461 tcagccctcg accacaacgc cgccccacgc ctccgggtcc acatcggcga cggactggct
     2521 aaacgtccag cggtggccgc cgatgtcttc ggcggtgtat tgccgctcgc caaaggggtg
     2581 agtggtgggt tcggtcagga tgcgcgcgcc ggcctgcacg gcccgggcac agtgggcgtc
     2641 cgcgttggcc acgcgcacca tgagacgttg gccaccgggg gcttcgtttg cgccatcggt
     2701 gacgacaatg gcctcgtcgc cgtaaacaag ttgccggcga tgagcgccga tgcgcagccg
     2761 ctcgcggaag ccaaaggcgg cgcacagcca ggccgcggcc acggccacat cggtataggc
     2821 cagttcgggg ataatcgcgc cgggcggcat cgaccggtta gccaggccgg gcggcggcac
     2881 agccagcccc acgccgccaa ccggctccgt cgtcagcgat tcgccaaagc cggcaatgag
     2941 cggccgcgcc tgcccgatgg cctcgcgcac ggccggcagc gacagcgagg cgcggtggct
     3001 ctcggcactg tcccacactt cggtgatgac gatggcgttc gggtcggcca ggtcggtggc
     3061 gatgacgtag ctcagacagc caggcaggtc gcggctactt tccagcagga tggccagcag
     3121 cgcgtcgcgc tcgccgggga tggctagtat cctaccgatc agtccgtaca tgatcactca
     3181 gtggcctatg gttggtgaga cctcagaggc cttccgaaac ctctgaggcc ctggcgacct
     3241 acttatcgtc cagcgccacc acgccgggca gctgccggcc ttccagcaac tccaggctgg
     3301 cgccgccgcc ggtgctgacg tgggtcatct tgtcggtcag gccggccttg cgcacggccg
     3361 cggccgagtc gccgccgccg atgatgacct tcgcgccgtg atcgaccatg ccggccagaa
     3421 tctgggccag ttggttggta ccgttggcga actgcggcgt ctcgaacacg cccatcggcc
     3481 cgttccagac gaccagttgc gccccttgca gctcggcatt gaagcgctcc accgttgccg
     3541 ggccgatgtc gagggccatc ttgtcgggcg agatggccca gaccatttcc acctcggagg
     3601 gcgcgtcgtt ggacacttcg gcggcgacga cgacatcgac cgggagaatg agctggtcgg
     3661 cggccatgtc cagcagccgc cgggcctccg gcagagcctc ttcctcgacc agcgaacggc
     3721 ccaggttgtg cccctgcgcc ttcaggaagg tgttggccat gccgccgccg atgaggatct
     3781 tgtcggcttt ctgcagcagg ttctcaatga gcttgatctt gtcgctgatc ttggctccgc
     3841 ccatgatggc cacgtagggg cgcggcgggt tgtcaacggc gatgctcagg gcagagacct
     3901 cctggcccat gaggaagccg gccacaaccg gcccgccctt agcccgcatg gcgtaggccg
     3961 cgccatcgac gctggcatcg ggccggtggg ccgagccgaa ggcatcatcg acgtagacgt
     4021 cggccagcga ggcgaaatcg gcccccaact gggcgtcgtt cttcttctcg cgtgggtcaa
     4081 agcgggtatt ctccagcaac aggacatcgc ccggttgcag cgccgccgcg gcggccttca
     4141 ccgcgtcgct catcacttcg gggacatagc gcaccggccg gccgagggct tcttccagct
     4201 tcttggccac gggggccatg ctgaactggc ggtcgtcggc cgtcttgggc cgccccagat
     4261 gagaggccag aatgagggcc gcgccgtgat cgagcaggta attgagggtc ggcagcgcgg
     4321 cgcggatgcg cgtgtcgtcg gtgacggtga tgttggcatt ggggtctttg ctgctcagcg
     4381 ggacgttgaa atcgac
//
LOCUS       DAOSMQ010000259         4384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20019_length_4384_cov_4.163779, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000259 DAOSMQ010000000
VERSION     DAOSMQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20019_length_4384_cov_4.163779"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK829_09365"
     CDS             <1..399
                     /locus_tag="PK829_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU41463.1"
                     /translation="RFVGLRGKGDSLARNLPYGDQRRLEIARALASNPKLLLLDEPTA
                     GMNPRETAETTDFIRNLRDEIGITILLIEHDMRVVMGISEDITVLDYGEKIAQGAPAD
                     IQSNPRVIEAYLGRGAAGAARLTVDSGEAA"
     gene            405..1112
                     /locus_tag="PK829_09370"
     CDS             405..1112
                     /locus_tag="PK829_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41464.1"
                     /translation="MLEINDVHAYYGKIHALKGISLSVEKGEIVTLIGGNGAGKTTTL
                     RTICALMRPSSGFVKLNGEDLNKYKPSQLAAKGVAMVPEGRGVFARMTVLENLEMGAF
                     HRNDKAGIAEDIERVYVLFPRLKERRGQVSGTLSGGEQQMLAMGRAMMSRPTLLLLDE
                     PSMGLAPVLVEAIFSTVEEINRQGTTILLVEQNALMALQIAGRGYVLQTGEIVLNDSA
                     KSLQRNAMVQKVYLGVE"
     gene            1127..2125
                     /locus_tag="PK829_09375"
     CDS             1127..2125
                     /locus_tag="PK829_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOU41465.1"
                     /translation="MELRLFSADDVDRALPMEQAIAAVRVAYVQLSAGRAQAALRAHL
                     DIAGGDSTLVMPFYVPAVSGADDGGALGLKVVSVFSSNPPRGLPLIHALVLALDGATG
                     APLALIEGSSLTAIRTGAASGVATDALARPDARSVALFGSGVQARRQLEAVCTVRAIE
                     RVRVYSLVGAEALAAEMAGHGPIPADISVTGSPREALAGADIVCTATTSRTPVFAGGD
                     LELGTHVNAIGSFTPEMQEIDAETVRRARVVIDSRAAALAEAGDLIIPLRDGVVALDH
                     VSDEIGEVLAGQRPGRTSAAQITFFKSVGVAVQDAAAAGLVVRNGAALNLGRVVTL"
     gene            2122..3282
                     /locus_tag="PK829_09380"
     CDS             2122..3282
                     /locus_tag="PK829_09380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU41466.1"
                     /translation="MSELPSTAEVVVIGGGVMGASTAYHLARRGLDGIVLLESQRFFG
                     QGATGKCAGGIRYQFSTAVNVRLSQLSLPMLARFEVETGQAIDLRYDGYMLLATTEEN
                     VAEFRRNVALQRSLGVPTEWFDGDEVRRRVPQLAADDVLAATFHAGDGLADPNGVVAG
                     YISAGRRLGVRAFTDAPVTAIDTASGHVTGVHTPLGHVATRTVVNAAGPWAAVVGQMA
                     GVALPIVPIRRQMLTTTPLPQLAPDFPFVIDFARSLYFHREGGGILTGMSNPRQPIGF
                     DESVDEAWELTHMEAAVARLPLLATAGRQAHWAGLYETTPDAHPIIGRVPGLGGFVVV
                     AGFSGHGFMHGPIAGLLVSEIVLDGGAHTLDIADLAYERFLEGGTMREYNVI"
     gene            3430..>4384
                     /locus_tag="PK829_09385"
     CDS             3430..>4384
                     /locus_tag="PK829_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU41467.1"
                     /translation="MKRGLAVLFVLLALAACGGEEPPANTLPQPTAPVAPTADLPIPA
                     DSDLVVVATDAPLPPYSDFDPFGNVVGFNAAVMDALAARAGFDYEFVVTPSDGVLDSI
                     AAGSARDFDAVMSALIIPDTPPPGIAFTQPYLEVGQVVVVLVDNKTIDSAASIQPGMA
                     VGVLADSAGQDAAAALGIDAADLFNQYERPSQLVQALIDEVVQAVILDSYTAEYFAAT
                     FPEQLRIAGGEGRAAWLDDRRYGIAVAADNTTLLDALNGALDGLRDSGEMEELALTWL
                     LPEVNAAAAVDPGESRVGTPTTELYIGVVGQFSDMDPASLTT"
BASE COUNT          691 a         1494 c         1500 g          699 t
ORIGIN      
        1 cgcttcgtcg ggctgcgggg caagggcgac agcctggccc gcaatctgcc ctatggcgac
       61 cagcggcggc tggagatcgc ccgcgccctg gccagcaacc ctaagctgct gctcctggac
      121 gaaccgaccg cgggcatgaa cccgcgcgag acggccgaga cgaccgattt catccgcaac
      181 ctgcgcgacg agatcggcat cactattctg ctcatcgagc acgacatgcg cgtggtcatg
      241 ggcatctccg aagacattac cgtgctcgac tacggtgaga agatcgctca gggcgctccg
      301 gccgatattc agagcaaccc gcgagtcatc gaggcctatc tcggccgtgg cgcggccggc
      361 gcggcgcgcc tgacggtcga ttctggggag gcggcatgac ggtcatgctg gagatcaacg
      421 acgtccacgc ctactatggc aagatccacg ccctcaaggg catctcgctc agcgtggaga
      481 agggcgagat cgtcactctc atcggcggca acggcgcggg caagaccaca acgttgcgca
      541 ccatctgcgc cctcatgcgg cccagtagcg gctttgtaaa gctcaacggc gaagacctga
      601 acaaatacaa gcccagccag ttggccgcca aaggcgtggc gatggtgccg gaagggcgtg
      661 gcgtcttcgc gcgcatgacg gtgctggaga acctggagat gggcgcgttc caccgcaacg
      721 acaaggcggg catcgccgag gacatcgagc gcgtctatgt gcttttccca cggctgaagg
      781 agcggcgcgg ccaggtgtcg ggcacgctct ccggcggcga gcagcagatg ctggccatgg
      841 gccgggcgat gatgtcgcgg ccgacgctgc tgctgctcga cgagccgtca atgggtctgg
      901 ctccggtgct ggtcgaggcc atcttcagca ccgtggaaga gattaaccgc cagggcacaa
      961 ccattttgct ggtggagcag aacgcgctca tggctctgca aatcgccggc cgcggctatg
     1021 tgctgcaaac gggcgagatc gtgctaaacg acagcgccaa gtcgctgcaa cgcaacgcga
     1081 tggtgcagaa ggtttatctg ggcgtggagt agcgcgctac ggccgtatgg agctacgtct
     1141 cttctccgcc gatgacgtgg accgggcgct gccaatggag caggccattg ccgcggtccg
     1201 agttgcctat gtgcagctat cggccggccg ggcgcaagcg gcgttgcggg cacacctgga
     1261 catcgccggc ggcgatagca cactggtgat gcccttctac gtgcccgccg tcagcggcgc
     1321 ggacgacggc ggcgcgctgg gtctgaaggt cgtctctgtc tttagcagca acccgccgcg
     1381 cggcctgccg ctcatccatg ccctcgtgct ggctctcgac ggggccaccg gcgcgccgct
     1441 ggccctcatt gagggtagca gcctgacggc catccgcacc ggcgcggcat cgggcgtggc
     1501 caccgacgcg ctagcccggc cggacgcgcg cagcgtggcc ctcttcggca gcggtgtgca
     1561 ggcgcggcgg caactggaag cggtgtgcac cgtgcgggcc atcgagcggg tacgggtcta
     1621 tagcctggtc ggggcggaag cattggccgc cgagatggcc ggccacggcc ccatccccgc
     1681 cgacatcagc gtgaccggca gcccacgcga ggccctggcc ggggccgata tcgtctgcac
     1741 ggcgacgacc tcccgcacgc ccgtctttgc cggcggcgac ctggagctgg gcacgcacgt
     1801 gaacgccata ggctcattca ctccggagat gcaagagatc gacgccgaga ccgtccggcg
     1861 ggcgcgcgtg gtcatcgact cgcgcgcggc ggcgctggcc gaagccggcg acctgatcat
     1921 tcctctgcgc gatggcgtcg tggcgctcga tcacgtcagc gacgagatcg gcgaggtgct
     1981 ggccgggcag cggccgggcc gcacgagcgc cgcgcagatc accttcttca agtctgtcgg
     2041 cgtggccgtg caagacgccg ccgccgccgg gctggtcgtg cgcaacggcg cggccctgaa
     2101 cctgggacga gtagtgaccc tgtgagtgag cttcccagca cggccgaagt cgtcgtcatc
     2161 ggcggcggcg tgatgggggc cagcacggcc taccatctgg ctcggcgcgg tctcgacggc
     2221 atcgttctgc tggagagcca gcgcttcttt ggccaggggg cgacgggcaa gtgcgccggc
     2281 ggcattcgct accagttcag cacagccgtt aacgtccgcc tgtcgcaact gagtttgccc
     2341 atgctggccc gtttcgaggt cgagacgggc caggccatcg acctgcgcta tgacggctat
     2401 atgctgctgg ccacgaccga ggagaacgtg gctgagttcc gccgcaacgt agccctgcaa
     2461 cgcagcctgg gcgtgcccac ggaatggttc gacggcgacg aagtgcgccg gcgcgtgccc
     2521 cagctggcgg ctgacgacgt gctggcggcg accttccacg ccggtgacgg cctggccgac
     2581 cccaatggcg tcgtcgccgg ctacatcagc gccgggcggc ggctgggcgt gcgcgcgttc
     2641 accgacgcgc cggtgacggc catcgacacc gccagcggcc acgtgaccgg cgtgcacacc
     2701 cccctgggcc acgtggccac gcggacggtg gtcaacgccg ccgggccgtg ggcggccgtc
     2761 gtcggccaga tggccggcgt ggccttgccc atcgtgccca tccggcggca aatgctgacg
     2821 acgacaccgt tgccgcaatt ggcccccgac ttccccttcg tcatcgactt cgcccgcagc
     2881 ctctacttcc accgcgaggg cggcggcatc ctgacgggca tgtccaaccc caggcagccc
     2941 atcggcttcg atgagagcgt ggacgaggca tgggagctaa cccacatgga ggcggccgtg
     3001 gcccggctgc cactgctggc cacagccggg cggcaggcgc actgggccgg tctttacgag
     3061 acgacgcccg acgcccaccc catcatcggc cgcgtgccgg ggttgggcgg ctttgtcgtc
     3121 gtcgccggct tctctggcca cggcttcatg catggcccca tcgccgggct gttggttagc
     3181 gagatcgttc tcgacggtgg ggcgcacacg ctcgacatcg ccgatctggc ctatgagcgc
     3241 ttcctggagg gtgggacgat gcgcgagtac aacgtcattt agcgccccca gcgccgacag
     3301 cgccgccctt aagctgtgta atctgtgaaa tccgtcgcct ctatttctgg cccatcccga
     3361 cgacggctat acttgggctg cggccgatgc cggtcgcagc gaggtctcat gatgccgtac
     3421 aggataaaga tgaagcgcgg cctggccgtg ttgttcgtgc tgctggctct ggctgcctgt
     3481 ggcggcgagg aaccgccggc caatacgctg ccccagccga ccgcgcccgt cgcgcccacg
     3541 gccgacctgc ccattcctgc cgacagcgat ctggtggtcg ttgccaccga cgcgcccctg
     3601 ccgccctata gcgacttcga cccgttcggc aacgtcgtcg gcttcaacgc cgcggtgatg
     3661 gacgccctgg ccgcccgcgc cggcttcgac tacgagttcg tggtcacgcc cagcgacggc
     3721 gtgctggata gcatcgccgc cggctcggcg cgcgacttcg acgcggttat gtcggccctg
     3781 ataatccccg acacgccgcc gccgggcatc gccttcaccc agccctatct ggaggtgggg
     3841 caagtggttg tcgttctggt cgataacaag accatcgaca gcgccgctag catccagccg
     3901 ggcatggccg tgggcgtgct ggccgacagc gccgggcagg acgccgccgc ggcgctgggc
     3961 attgacgctg ccgacctgtt caaccagtat gagcggccgt cgcaactggt acaggcgctc
     4021 attgacgagg tggtgcaggc tgtcattctt gatagctaca cggccgagta cttcgccgcg
     4081 accttcccgg agcagttgcg cattgccggc ggcgaggggc gcgccgcctg gctggacgac
     4141 cgccgctacg gcattgccgt ggccgccgac aacaccacgc tgctcgacgc gctcaacggc
     4201 gcgctcgacg gcctgcgcga tagtggcgag atggaagagt tggccctgac ctggcttctg
     4261 ccggaggtca acgccgcggc ggccgtcgac cccggcgagt cgcgcgtggg cacgccgacg
     4321 accgaactat acatcggcgt cgtcggccag ttcagcgaca tggacccggc cagcctgacg
     4381 accg
//
LOCUS       DAOSMQ010000260         4381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20040_length_4381_cov_4.421405, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000260 DAOSMQ010000000
VERSION     DAOSMQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20040_length_4381_cov_4.421405"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PK829_09390"
     CDS             complement(<1..781)
                     /locus_tag="PK829_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41468.1"
                     /translation="MKAMVDRSAGPSDDSHFLSSGSERPPRADALLELQRLLEVLFPG
                     PPSLDCITVDDVLRRYPLTGLGAPALTAIEQSQRIVRARGDFSQTGLGEFHIGLIYFH
                     WHDYRAAAAQFALARQPWTLAGDHSANCLARFAQGQALAQANHHEPAMLQFGRVERLL
                     ARPVHGAAGTRHAQLSEKLWPLLTTAQEGLREKLWPEDRPPGVVKGGATRPAPARHES
                     ASAGQTPPPASQAEARHESAGAGQAPPPSGRATRQEPPRSSE"
     gene            complement(786..2210)
                     /locus_tag="PK829_09395"
     CDS             complement(786..2210)
                     /locus_tag="PK829_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU41469.1"
                     /translation="MGPQVHAQAAPSAPAQSVRRRSRWTFSWFTWPRLILLALLLGYW
                     FFVSRLERVEGNIVLAGFFLPDLPTGQAAAVTFSPWLEVVVELFHPRVLRHLIPLFVG
                     WYLAVQAAVSLMQVLYDCPDRETAAAFLSRQRRDRVSGMELPFIVLPHTLESIREESI
                     LVRVGGPVQVIIPNGYAAVTERNARFLRVLPPGFHVLGRFEYLLGVIDLQPQSRSAEG
                     VSMLTREGIQMKADIGLTFCIDPGDEPSPLRPYPFRPEAVRRAAYGGAVGGNGKVSTW
                     DGGPLGRAIGALSGWVSNHTVDELLAADASHDAHHLLIQAIIDKVWDKDGLLKDGIKP
                     LRVHISRLTPPEPVSKQYTEVWLAAQRKEDLLLQAGDAAQLTKDLETLHDEGQLLIVQ
                     TITESIRRMQQEAGSRVSGYVLAMSLVEALQKMFHLSVDKARAASADTGRLEQEIEYV
                     GKRLASLEERLKSPTVDFNSLSPD"
     gene            complement(2215..3480)
                     /locus_tag="PK829_09400"
     CDS             complement(2215..3480)
                     /locus_tag="PK829_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU41470.1"
                     /translation="MEEPRRQFIPGLGILLLSAILLGMVGALLHDFRGVGYGRVGALI
                     WAGGVLITFVLGTAYLSRRLLPLQGNSGWGEGYRLLWRSYLLGVNNLLSGVRHEPLGS
                     ARPKKTAVSALSPSFQLLGAGFLFSHEAAAITRGNSFMRAAGPGLVLLQPGETIAKVF
                     DLRTHSRKQSVSATTREGIPVETSVTVSFHVRRLSPNERRPRSVETDAIPYPYDRQAL
                     FDLTYAGAVAGENHLDWTEQVAPQAATLLVGEIGRYTLDQLLESGGAGPLGEIKANIK
                     RGLEALQTSEEGPTLPKGITIVSVGVGGLEPPEDVARKRLETWQVKWQAQAEKAIADG
                     KAEGLRLINQARAQALSDAIDSLLTSIEAAQNLGQSPLPDVILSQVVATLEGLAANQA
                     LHNLPQNTQWLSDASDASREIRYLLGPEE"
     gene            3555..>4381
                     /locus_tag="PK829_09405"
     CDS             3555..>4381
                     /locus_tag="PK829_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOU41471.1"
                     /translation="MHVAINATFWGRPNTGSGQYTRQLVTHLRQLVSDLDLTLVYPQP
                     PGGAPPEDVPAGVNVAVVPLRPGHAGKVLFEQRGFPAACRRVGATLAHVPYFGPPLRS
                     PVPLVVTVHDLTTLLVPEYRRRAGARLYNALVSAAARGAGHVLTDSQASKADILAHLG
                     LADGDVTVAYLGVGPEYTPRAGNSLVDLAVRRKYDLPDFYVLYLGGYETHKNVVTLLH
                     AYTYVAQALGEDYPLLLAGQKPAGASPTFPDYDAIIARAGLGRFVRWLGYVEEADKPV
                     "
BASE COUNT          704 a         1500 c         1451 g          726 t
ORIGIN      
        1 actccgacga gcgcggcggc tcctggcgcg tcgcccggcc cgacggtggc ggcgcttggc
       61 ccgcgccggc gctctcgtgg cgcgcttccg cctggcttgc tggcggcggc gtctggcccg
      121 cgctggcgct ctcgtggcgc gccggcgcgg gccgtgttgc gccgcccttg accacccctg
      181 gcggccgatc ttccggccag agcttctcgc gcaacccttc ctgcgccgtc gtcagtagcg
      241 gccacaactt ctcgctcaat tgcgcgtggc gcgtgcccgc cgcgccatgg acgggccggg
      301 ccagcaaccg ctcgacgcgg ccgaactgga gcatggccgg ctcgtggtgg ttggcctggg
      361 ccagggcctg gccctgggca aagcgcgcca ggcagttggc tgaatggtcg ccggccagcg
      421 tccacggttg gcgggccagg gcgaactggg ccgccgcggc gcggtagtcg tgccagtgga
      481 agtagatcag gccgatgtga aattcaccca ggcccgtctg gctgaaatcg ccgcgagcgc
      541 ggacgatccg ttgcgactgc tcgatggccg tcagcgcggg cgcgcccagc cctgtcaggg
      601 gatagcgccg caagacatca tccacggtaa tgcaatcgag ggagggcggc ccggggaaca
      661 gcacctccaa caggcgttgc agttccagga gagcgtcggc gcgaggcggt ctctccgaac
      721 cagagcttag gaagtgggaa tcgtcgctgg ggccggcgga gcggtcaacc atggccttca
      781 catgcctagt ccggactgag ggagttgaaa tccaccgtcg gcgacttcag cctttcctcc
      841 aggctggcca gccgcttgcc gacgtactcg atctcttgtt ccaggcgacc tgtgtccgcg
      901 ctggcggctc tggccttatc cacactgagg tgaaacatct tctgcaacgc ctctaccagg
      961 ctcatggcta agacatagcc tgatacccgg ctgccggctt cctgttgcat ccggcggatc
     1021 gactcggtga tggtctggac gatcaggagt tgcccctcgt catggagcgt ttccaggtct
     1081 ttggtcagtt gcgctgcgtc gccggcctgt aagagcaagt cctcctttcg ttgagcagcc
     1141 aaccacactt ccgtatactg cttgctgacc ggctccggcg gcgtcagccg gctgatgtgg
     1201 acgcgcaggg gtttgatgcc gtccttcagc aggccgtcct tgtcccagac cttgtcgatg
     1261 atggcctgga tcaggagatg atgggcgtca tgggaagcat cggcggcaag caactcgtcg
     1321 accgtgtggt tcgacaccca accgctcagc gcgccaatag cccggcccaa cggcccgccg
     1381 tcccaggtcg agaccttgcc gttgccgccc accgcgccgc cataggcggc cctgcgcacg
     1441 gcttcgggtc ggaacggata gggccgcagc ggcgacggct cgtcgccggg gtcgatgcag
     1501 aaggtcaggc cgatgtcggc tttcatctgg atgccctcgc gggtcagcat ggacacgcct
     1561 tcggcgctgc gcgattgcgg ttgcaggtca atgacgccca gcaggtactc aaaacgcccc
     1621 aggacgtgga agccgggcgg caagacgcgc aagaagcggg cgttgcgctc ggtcacggcg
     1681 gcatagccgt tggggataat cacctgcacc ggcccgccga cgcgcaccag gatcgactct
     1741 tcgcggatgc tctccagcgt gtgcggcaag acgataaagg gcaactccat gccgctcacc
     1801 cggtcgcggc gctgccggct aagaaaggcc gcggccgtct cgcggtcggg gcaatcgtag
     1861 agcacctgca ttaagctcac agccgcctgc acggccagat accagcccac aaagagaggg
     1921 atcaaatgac gcaggacgcg cggatggaac agttcgacga cgacctctag ccagggcgaa
     1981 aatgtcaccg ccgccgcctg ccccgtgggt agatcgggca ggaagaagcc cgccaggacg
     2041 atattgcctt cgacacgctc tagcctgctc acgaagaacc agtagcccag caataaggcc
     2101 aggaggatta gccgcggcca ggtaaaccag ctgaacgtcc aacgtgaccg ccggcggaca
     2161 ctctgggcgg gagcgctcgg cgcggcctgg gcatgcacct gtggccccat gaccctactc
     2221 ctctggcccc aggaggtagc ggatttcacg cgaggcgtca gatgcatcgc tgagccactg
     2281 cgtgttctgc ggcaagttgt gcagcgcctg gttggccgcc agtccctcca gcgtcgcaac
     2341 cacttgcgag aggatgacat cgggcagcgg cgactgcccc aggttctgcg cggcctcaat
     2401 gctggtcaac aggctgtcga tggcgtcgga cagagcctgg gcgcgggcct ggttgatcag
     2461 gcgcagcccc tccgctttgc cgtcggcgat tgccttttcg gcctgggcct gccacttgac
     2521 ctgccacgtc tccagccgct tacgggctac gtcctccggc ggctccagac cgcccacgcc
     2581 gacactgacg atggtgatgc ccttgggcag cgttggcccc tcctcgctcg tttgcagcgc
     2641 ttccagcccg cgcttaatgt tggccttgat ctcgcccagc ggcccggcgc cgccgctttc
     2701 gaggagctgg tcgagtgtat agcggccgat ctcgcctacc agcagcgtcg ccgcctgggg
     2761 agcgacctgc tctgtccaat cgagatggtt ctcgccggcg acagcgccgg cataagtcag
     2821 atcgaagagc gcctggcggt cgtagggata ggggatggcg tccgtctcta ccgagcgcgg
     2881 ccgccgctcg ttgggcgaca gccggcggac gtgaaaggac acggtcacgc tggtttccac
     2941 ggggatgccc tcgcgggtgg tggcgctcac ggactgtttg cgcgagtgcg tgcgcaggtc
     3001 gaagaccttg gcaatggtct cgccgggctg caacagcacc agccccggcc cggcggcgcg
     3061 cataaagctg ttgccgcgcg tgatggccgc ggcctcgtgg ctgaacagga agccggcccc
     3121 taatagctga aagctgggcg acagcgcaga caccgcggtc ttcttgggcc tggccgagcc
     3181 aagcggctcg tggcgcacac cgctcagcaa gttgttgacg cccagcagat agctgcgcca
     3241 caacaggcga tagccctcgc cccagccgct gttgccctgc aggggcagca gtcgccgcga
     3301 gaggtaggcc gtgcccagca caaacgtaat cagcacgccg cccgcccaga tgagcgcgcc
     3361 gacgcggccg tagcccaccc cgcggaagtc gtgcagcaac gcgccgacca tgcccaacag
     3421 gatggccgac aggagtagga tgcccaggcc gggaatgaac tgtcttcttg gctcttccat
     3481 gtggtttaag gccactcgcc tgcgcaaact atatcatagg ccatagggtg ggcaaagggg
     3541 tataatcccg gtcgatgcac gtcgccatca acgccacttt ctggggccgg cccaacaccg
     3601 gcagcggcca gtacacccgc caactcgtca cccacttgcg gcaactggtg tcggacctgg
     3661 accttaccct ggtctacccc cagccgccag gcggcgcgcc gccggaagac gtgcccgcgg
     3721 gcgtgaatgt tgccgttgtg cccctgcggc ccggccacgc cggcaaggtt ctgttcgagc
     3781 agcgcggctt cccggccgcc tgccggcgcg tgggggccac gctggcccac gtgccctact
     3841 tcggcccgcc gctgcgttcg cccgtgccgc tggtggtcac cgtccacgac ctgacgacgc
     3901 tgctcgtgcc cgaatatcgc cgtcgcgccg gcgcccggct ctataacgcc ctcgtcagcg
     3961 ccgcggcgcg tggggccggc cacgtgctga ccgattcgca ggccagtaag gccgacatcc
     4021 tcgcccatct gggcctggcc gacggggatg taaccgttgc ctacctgggt gtcggcccgg
     4081 agtatacgcc gcgggcgggc aacagcctcg tcgatctggc cgtccggcgc aaatatgacc
     4141 tgcccgactt ttacgtcctc tacctgggcg gctacgagac ccacaagaac gtcgtcaccc
     4201 tgctgcacgc ctacacctac gtggcccagg cgttgggcga ggactacccg ctgctgctgg
     4261 ccgggcagaa gccggccggc gcgtcgccca ccttccccga ctacgacgcg atcatcgcgc
     4321 gggccgggct ggggcgcttt gtgcgctggc tcggctacgt cgaggaggcc gacaagccgg
     4381 t
//
LOCUS       DAOSMQ010000261         4319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20421_length_4319_cov_4.113508, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000261 DAOSMQ010000000
VERSION     DAOSMQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20421_length_4319_cov_4.113508"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..616
                     /locus_tag="PK829_09410"
     CDS             101..616
                     /locus_tag="PK829_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41472.1"
                     /translation="MTPLYREEQLYRELPPFTLLVVVGTVFGWLLVVWAGLLGRPIGQ
                     MTLPLPLALAIGLPLGLLLPLAYGRLRMTTEVFPDRIVVHNGMSSNLSLPLANVVDVV
                     VRTDDIRGDYNQRNVGVVSNTRVAYVVDAVQGVQLEARDGRLFLIGSREPAALAAALA
                     AARGQPVAASE"
     gene            624..1121
                     /locus_tag="PK829_09415"
     CDS             624..1121
                     /locus_tag="PK829_09415"
                     /inference="COORDINATES: protein motif:HMM:NF039358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6141 family protein"
                     /protein_id="HOU41473.1"
                     /translation="MRATHPVLFHEVQRFRDVWWVMVLVFGVATTQWFAFLSQLLDGS
                     WRGNVVGLAALGLFWLLFGVGLPLFFLLLRLEVDVYPQTVVIRFAPLLERTIPKLEIA
                     GIEVRQYQPLGEFGGWGIRGFGGRIAYNVRGNQGVELTLTDGRRVLIGSRRAGELAAA
                     IRSIG"
     gene            1359..2771
                     /locus_tag="PK829_09420"
     CDS             1359..2771
                     /locus_tag="PK829_09420"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HOU41474.1"
                     /translation="MSDHIPQTMGQQHGRRSWAEPWKIKMVEPLAVISADERRAALEA
                     AGFNTFLLRSDDVYIDLLTDSGTNAMSDRQWAGLMLGDEAYAGSRNFYHLEEAIQSVY
                     GYRYVVPTHQGRGAEHLISRAAIRPGQYVPGNMYFTTTRLHQEMAGGIFTDVIIDEAH
                     DPANLHPFKGNISLDKLAAVVDAHGADGVAYVSLAATVNMAGGQPVSMANLREVRAFC
                     DRHGIKLYLDATRLVENAFFIQEREEGYAGKPVAAIVREFCDLTDGAWMSGKKDNLVN
                     IGGWLAVNDQALFEEVRNLVVVFEGLHTYGGLAGRDMEAMAIGIRESVADEHIRSRVG
                     QVRYLGELLTQWDIPIVQPVGGHAIFLDARAFYPQLPQIEFPAQTLAAELYLDSGIRS
                     MERGIASAGRDAKTGDHYYPKLELTRLTIPRRVYTQAHMDVVAESVKAVYDARATTRG
                     LRMVYEPKYLRFFQARFERL"
     gene            2891..3625
                     /locus_tag="PK829_09425"
     CDS             2891..3625
                     /locus_tag="PK829_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU41475.1"
                     /translation="MKIIALYNIKGGVGKTAAAVNLAYLSAGEGTPTLLWDLDPQGAA
                     SYCLRVDARLKGGRKGLLRGKHPIHQLTQPTAYAGLDVLPADFSYRNMDIDLSEGKRP
                     ERRFRKLLEPFERDYQTIFLDCPPGLTLSTESMFGAADVLVVPVIPSTLSLRTLALVD
                     DFLAQRELTQLRVAAFYSMVDRRKTLHRQLVEARHDGRELFTTIPYASDVEQMAERRA
                     PVAEYAPRSAAALAFGQLWSELKEVI"
     gene            complement(3746..>4319)
                     /locus_tag="PK829_09430"
     CDS             complement(3746..>4319)
                     /locus_tag="PK829_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41476.1"
                     /translation="QIVENRVDRAQEAAAHAGAQVTTLESTVESLESLSGGLEAADDP
                     DAAARAAAAEARLAELEPRLRAAEAAAARAETRAAEQATQLAVIQAEAVGELHANASP
                     ADARWSDMLRGDTVANFAFADLGKIQMFLFTVVLVFAYAALVWSIMRMPAAEGVLQVV
                     PSLSLPAFSDSAIITLALSHGGYLATKTAA"
BASE COUNT          675 a         1455 c         1448 g          741 t
ORIGIN      
        1 aagtgtatca taaatgcctg ttaatttgtt aatgtcctaa caggctgtgc tacggcctgt
       61 gctactggcc atgacgctcc catcgcgcta tcatagcgcc atgacgcctc tgtatcgcga
      121 agagcagctc taccgcgagt tgccgccgtt cacactcctg gttgtcgtcg ggacggtctt
      181 cggctggctg ctggtggtct gggcgggtct gctggggcgg ccgatcgggc agatgacttt
      241 gcccctgccg ctggcgttgg ccatcggtct gcccctgggc ctcctgttgc cgctggccta
      301 cggtcgcctg cgcatgacca ccgaggtctt ccccgaccgc atcgtcgtcc acaacggcat
      361 gagcagcaac ctgagcctgc ccctggccaa tgtcgtcgat gtggtcgtgc gcaccgacga
      421 catccgcggc gactacaatc agcgcaacgt cggcgtggtc agcaatacgc gcgtcgccta
      481 cgtcgtcgat gccgttcagg gcgtccagtt agaggcgcgc gacggccgcc tgttcctcat
      541 cggctcgcgc gagccggcgg cgctggcggc ggcgctggcg gcggcgcgtg gccaacccgt
      601 ggccgcgtcc gagtgagggg tgcatgagag cgacacaccc agtcctgttt cacgaagtcc
      661 agcggttccg cgacgtctgg tgggtcatgg tgctggtgtt cggcgtggcc acgacacagt
      721 ggttcgcctt cctcagccag cttctggacg gctcctggcg cggcaacgtg gttggcctgg
      781 ccgccctggg cctcttctgg ctgctgttcg gcgtcggcct gccgctcttc ttcctgctcc
      841 tgcggctgga ggtcgatgtc tacccccaga cggtcgtcat ccgcttcgcg ccgctgttgg
      901 agcggaccat tcccaagctg gagatcgccg gcatcgaggt gcgccagtat cagccattgg
      961 gcgagttcgg cggctggggc attcgcggct tcggcgggcg catcgcttat aacgtgcgcg
     1021 gcaaccaggg cgtcgaattg acgttgaccg acggccggcg cgtcctcatt ggctcgcggc
     1081 gggccgggga gctggcggcg gcgattcgtt cgattggata ggtcagcagc gacgcagggg
     1141 agggcgaaca gggtttcaat tgtcggcccc ggcgcgccga cctgtcaggt gtcaggtcac
     1201 tggtcgccag acgaatctgg caaagccctg catggtaaag gttcgcccgg cagttgtaaa
     1261 tcggtgtgat acaattgctc tcatcccacg ccgtggtggc ctcgacctgt cggccaggcc
     1321 cgcccggcga caaccccgaa ttgcgccaga ggaggcatat gtccgaccac attccgcaga
     1381 ccatgggcca gcaacacggc cgccgctcct gggccgagcc gtggaagatc aagatggtgg
     1441 agccgctggc cgtcatctcg gccgacgagc gccgcgccgc tctggaggcc gccggcttca
     1501 acacctttct gctgcgctcc gacgacgtct acatcgactt gctgaccgac agcggcacaa
     1561 acgccatgag cgaccggcaa tgggccgggc tgatgctggg cgatgaggcc tatgccggca
     1621 gccgcaactt ctaccacctg gaggaggcca tccagagcgt ctacggctac cgctacgtcg
     1681 tgcccacgca ccaggggcgc ggcgcggagc acctcatcag ccgcgccgcc atccggccgg
     1741 ggcagtatgt gcccggcaat atgtatttca cgaccacgcg cctgcaccag gagatggccg
     1801 gcggcatctt caccgacgtc atcattgacg aggcccacga cccggccaac cttcacccct
     1861 tcaagggcaa tatcagcctg gacaagctgg ccgccgtggt agacgcccac ggcgcggacg
     1921 gcgtggccta cgtcagcctg gcggccacgg tcaacatggc cggcggccag ccggtcagca
     1981 tggccaacct gcgcgaggtg cgcgccttct gcgaccgcca cggcatcaag ctctatctgg
     2041 acgcgacgcg cctggtcgaa aacgccttct tcatccagga gcgcgaggag ggctatgccg
     2101 gcaagcccgt cgccgccatt gtccgcgagt tctgcgacct gaccgacggc gcgtggatga
     2161 gcggcaagaa ggacaacctg gtcaatatcg gcggctggct ggccgtcaat gaccaggcgt
     2221 tgtttgagga agtccgcaac ctggtcgtcg tcttcgaggg gctgcacacc tatggcggcc
     2281 tggccgggcg cgacatggag gccatggcca tcggcatccg cgagtcggtg gccgatgagc
     2341 acatccgctc ccgtgtgggc caggtgcgct atctgggcga gttgctgacg cagtgggaca
     2401 tccccatcgt ccagccggtc ggcggccacg ccatcttcct ggacgcgcgc gccttctacc
     2461 cgcagttgcc ccaaattgag ttcccggcgc agacgctggc ggccgagttg tacctcgact
     2521 ccggcatccg ctcgatggag cgcggcatcg ccagcgccgg ccgcgacgcg aagacgggcg
     2581 accactacta tcccaagctg gaactgacgc gcttgaccat cccgcggcgc gtctacaccc
     2641 aggcgcacat ggacgtggtg gccgagtcgg tcaaggccgt ctacgacgcc cgcgcgacca
     2701 cgcgcggcct gcgcatggtc tacgagccga agtacctgcg cttcttccag gcgcgtttcg
     2761 agcgcctgta gctgtagcgc aggctgttag cctgcctctt ccctgtaggg cgggttggca
     2821 acccgcctct gacccgcccg tccggcaagg aggcggggtt accaacccgc cctacaatta
     2881 ggagaaatcg ttgaagatca tcgccctcta caatattaag ggcggcgtgg gcaaaacggc
     2941 cgcggccgtc aacctggcct acctgtcagc cggcgagggc acgccgacgc tgctgtggga
     3001 tctcgacccg cagggcgcgg cgtcctactg cctgcgcgtc gatgcccggc tgaagggtgg
     3061 gcgcaagggg ctgctgcggg gcaagcatcc cattcaccaa ctgacgcagc cgacggccta
     3121 tgccggacta gacgtgctgc cggcggactt ctcctaccgc aacatggaca tcgacctgag
     3181 cgaggggaag cggccggaac gacgcttccg caagctgctg gagccgttcg agcgcgatta
     3241 tcagaccatc ttcctcgact gcccgccggg cctgaccctt tccacggaga gcatgttcgg
     3301 cgcggccgat gtgctggtgg tgccggtcat cccgtccacg ctgtcactgc ggacgctggc
     3361 gctggtcgat gacttcctgg cgcagcggga gctgacgcag ctgcgcgtgg cggccttcta
     3421 tagcatggtt gaccgccgca agacgttgca ccggcagctg gtggaggcgc gccacgacgg
     3481 ccgtgagctg ttcacgacca tcccctacgc cagcgacgtg gagcagatgg ccgagcgccg
     3541 cgcccccgtg gccgaatacg ccccgcgctc ggccgccgcc ctcgccttcg gccaactgtg
     3601 gagcgagtta aaggaagtca tttaggggat aggggccagg ggccaggttc caggggccag
     3661 cggccagggc aggacgcttt gcgccggcgc atactcgctg gatagcgttc ttccctagcc
     3721 cctagcccct agcccctatc cccttctacg ccgccgtctt cgtggccaaa tagccgccgt
     3781 gggagagggc cagagtgatg atggcgctgt cggagaaggc cggcaggctg agcgagggca
     3841 cgacttgcag cacaccctcg gccgcgggca tgcgcatgat cgaccacacc agcgccgcgt
     3901 aggcaaagac cagcacaacg gtgaacagga acatctgaat cttgcccagg tcggcgaagg
     3961 cgaagttggc cacggtgtcg ccgcgcagca tgtcgctcca acgggcgtcg gccgggctgg
     4021 cgttggcgtg cagttcgccg acggcttcgg cctggatgac ggccagttgc gtggcctgtt
     4081 cggcggcgcg cgtttcggcg cgggcggcgg cggcctcggc ggcgcgcagg cgcggctcca
     4141 actcggccag gcgcgcctcg gccgcggcgg cgcgcgccgc cgcgtcgggg tcgtcggccg
     4201 cttccagccc gccgctcagc gactccaacg actcgacggt cgattccagg gtcgtgacct
     4261 gcgctccggc gtgggcggcg gcctcctggg cgcggtcgac gcggttctcg acgatctgg
//
LOCUS       DAOSMQ010000262         4310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20475_length_4310_cov_4.951116, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000262 DAOSMQ010000000
VERSION     DAOSMQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20475_length_4310_cov_4.951116"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PK829_09435"
     CDS             <1..251
                     /locus_tag="PK829_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOU41477.1"
                     /translation="PTHILIVPNKPIPTVNDLTDADEAIAGRMLRVAARVAAQEGIAE
                     DGYRLIINCNRNGGQEVYHLHMHLLGGRRMGRMVMPPA"
     gene            complement(269..1855)
                     /locus_tag="PK829_09440"
     CDS             complement(269..1855)
                     /locus_tag="PK829_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU41478.1"
                     /translation="MGRQNATACHRLLFLCLLLCLSACVRPGPRPPAAVEVTPAGAPA
                     DSLNATGDAIDTTASEPVAAQLGTPVPAYAGQPTPDAPHYEVTTGSETAVGSHVVAAG
                     ETLGTIAQRYDTTVEALIELNGLANPDVISVGQQLQVPGVAIQRVVGPEFKIVPDSEL
                     VYGPGAAGFDVRAFTAPLGGRLLGYQEAVEGQTMDGPAIVQLVADRHSVNPRLLLAVL
                     EYKAGWVTDAQPPERANLLGYIGTTVPAGLYVQLSWAANLLNLGFYGRNEGGLRGFNL
                     GETTQVTFAPTINDGTAGVQRFLGAVEGITAEAWQRDAGPDGLYATYVSLFGNPFGYA
                     HEPLLPAGLAAPPLELPWAKGETWYLTGGPHGAWNSGSAWGALDFVPPSDITGCVQTD
                     AWVTAPAAGVVVRSGNGAVVIDLDGEGEAADGYAGTGWAITAMHLETRDRIANGARVA
                     VGDRLGHPSCEGGFSNGTHVHLVRSYNGRWISADGDIPFDLSGWVSSGAGAEYDGYLT
                     RGEAVRGACVCREDVNAINH"
     gene            2009..2674
                     /locus_tag="PK829_09445"
     CDS             2009..2674
                     /locus_tag="PK829_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOU41479.1"
                     /translation="MQAPRPAQRLRLTFSKSGPARYISHLDLARALERSLNRAGLPVA
                     YSQGFNRRPRLSLAAALPLGYTSAAEVADVWLTEPVAVEVFQERLAARLAPGLGLLAV
                     EETPLAAPSAQGQLVGAAYEVAFPEGVDAVALREAVAGVLAAGRLMRDRHRAKDTRPQ
                     PFDLRPLILAMRVADDDDGPRLYLELAHSPTATGRPDDVLSALGLDPLAAHVHRVALR
                     FAG"
     gene            complement(2671..3741)
                     /locus_tag="PK829_09450"
     CDS             complement(2671..3741)
                     /locus_tag="PK829_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41480.1"
                     /translation="MLATSLILLAVAAVVYFVNPFVRLALETASLARPVSTPDVAALN
                     AADSGNWCVRGDFADDAQPRLLDDGSQGDVLANDAVFSLEYTIAQPGHYLWQVVDCSD
                     TTRAFPASMAWITTEQPNQPVTFLFDSNERADRLFFPIPYAVTALDSATDFHIIGSFQ
                     DWIVDDASGRLEPLGGGGIYQQVRRIARSGNYQSYVIAGDPKRAIDAYGRTTEPIPFA
                     FDTDHNGDYVVFLVDIDRGRASVMYDMPPLMTKLAFGGGHLRLSEALAGLAALLLVAL
                     LLRELTLRNRRLWMETGCPRCHEQELMRISRRPKERLLHLLAIPAYRYRCRNCTWEGT
                     RLSEAGRSISPGATIAHIEGLR"
     gene            3805..3948
                     /locus_tag="PK829_09455"
     CDS             3805..3948
                     /locus_tag="PK829_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41481.1"
                     /translation="MRGAWWALGAGGAGALGFAAGGRDGWLGQFGAAGGHAFFKQILI
                     SQF"
BASE COUNT          665 a         1410 c         1556 g          679 t
ORIGIN      
        1 cccccaccca catcctgatc gtccccaaca agcccatccc caccgtcaac gatctgaccg
       61 acgctgacga ggccatcgcc gggcggatgt tgcgcgtggc cgcgcgggtg gcggcccaag
      121 agggcatcgc tgaggatggc tatcggctga tcatcaactg caaccgcaat ggcgggcagg
      181 aggtctatca cctgcatatg cacctgctgg gcgggcggcg gatggggcgc atggtgatgc
      241 cgccggcctg actgtcggcc gggcagcgct aatggttgat ggcgttgacg tcctcgcggc
      301 agacgcacgc gccgcggacg gcctcgccgc gggtcaggta gccgtcgtac tccgcgcccg
      361 cgcccgacga cacccagccg ctcaggtcaa aggggatgtc gccgtcggca ctgatccagc
      421 ggccgttgta gctgcgcacc agatggacgt gcgtgccgtt ggagaagccc ccctcgcagg
      481 acgggtggcc gagccggtcg ccaacagcca cgcgcgcgcc gttggcgatg cggtcgcgcg
      541 tctccaggtg catggccgtg atggcccagc ccgtgccggc gtagccgtcg gccgcctcgc
      601 cctcgccgtc caggtcgatg accaccgcgc cgttgccgct gcgcacgacg acgccggccg
      661 cgggcgctgt tacccaggcg tcggtctgaa cgcagccagt gatgtcactg ggcggcacga
      721 agtccagcgc gccccaggcc gagccggagt tccacgcgcc gtgggggccg ccggtcaggt
      781 accaggtctc gcctttggcc cagggcaact ccagcggcgg cgcggccagg cccgccggca
      841 gtagcggctc gtgggcatag ccgaacgggt tgccgaacag gctcacgtag gtagcgtaga
      901 gaccatcggg gccagcgtcg cgctgccagg cctcagcggt gataccctcc accgcgccga
      961 ggaagcgctg cacgccggcc gtgccatcat tgatcgtcgg cgcgaaggtg acctgggttg
     1021 tctcgcccag gttgaagccg cgcaggccgc cttcgttgcg gccgtagaag cccagattga
     1081 gcagattggc cgcccaacta agttggacgt aaaggccggc ggggacagtt gtgccgatgt
     1141 agccgagtag attggcgcgc tcaggcggct gggcgtcggt cacccagccc gccttatatt
     1201 ccagcacggc cagcagcagc cgcgggttga cactgtggcg gtcggccacc agttgcacaa
     1261 tggccgggcc atccatcgtc tgcccctcca ctgcctcctg atagccgagc aagcgtccgc
     1321 ccagaggcgc ggtgaaggcg cggacgtcga aaccggccgc gccggggcca tagaccaatt
     1381 ccgaatcggg cacaatcttg aactccggac cgacgacgcg ctggatcgcc acgccgggca
     1441 cttgaagctg ctggccgaca ctgatcacgt cgggattggc caggccgttc agttcgatga
     1501 gcgcctcaac ggtcgtgtcg tagcgctggg cgatagtgcc cagcgtctcg cccgcggcga
     1561 cgacgtggct gccgacagcg gtctcgctgc ccgtggtgac ttcgtaatgg ggagcgtcgg
     1621 gagtgggctg gccggcgtag gcaggtaccg gcgtgcccag ctgggcggcg acgggttccg
     1681 aggcggtcgt gtcgatggcg tcgcccgtgg cgttgaggct gtccgcgggc gcgccggccg
     1741 gcgtgacctc gaccgcagct ggcgggcgcg gccccggccg cacgcaagcg gaaagacaca
     1801 atagtaagca caggaagagc agacggtggc aggcagtggc gttctggcgt cccatcccgc
     1861 ctattctatc cgagcgggcg ggtcatgcca tgcccaatga cctgtcaggt cttcgagcaa
     1921 ctgatgactt tggaaaaacc ccgtgtcagg gggcgtgccg tttcccattc cctgggaacg
     1981 tggtatcaat gcgggcgtga caaccgacat gcaagcccca cgtccggcgc aacggttgcg
     2041 gctgaccttc agcaagagcg gtccggcgcg ctacatcagc cacttggacc tggcccgggc
     2101 gctagagcgc tctctgaacc gggccgggct gcctgtggcc tatagccagg gcttcaaccg
     2161 ccggccgcgc ctgtcattgg ccgcggccct gccgttgggc tacaccagcg cggccgaggt
     2221 ggccgacgtc tggctaacgg agccggtcgc agtcgaagtc tttcaggagc ggttggcggc
     2281 gcggctggcc cccgggctgg ggctgctggc tgttgaggag acgccattgg ccgcgccgtc
     2341 ggcgcagggg caactcgtgg gcgcggccta cgaggtggct tttccggaag gcgtcgacgc
     2401 ggtggccctg cgggaggcag tggcgggcgt gctggccgcc gggcgtctga tgcgcgaccg
     2461 ccaccgggcc aaggacaccc gaccgcagcc gtttgacctg cggccgctca tcctggccat
     2521 gcgcgtggcc gacgacgacg acgggccgcg cctttacctg gaactggcgc actcgcccac
     2581 ggccacgggc cgcccggacg acgtcctgtc cgcgctgggc ctggatccgt tggccgccca
     2641 cgtccaccgc gtggccctac gcttcgccgg ctaacgcagc ccttcgatgt gggcgattgt
     2701 cgccccagga gagatcgacc gccccgcttc gctcaggcgc gtgccttccc aggtgcagtt
     2761 gcggcagcga tagcgatagg cggggatggc cagtagatgc agaaggcgtt ccttgggacg
     2821 gcggctgatg cgcatcagtt cctgctcgtg gcaacgcggg cagcccgtct ccatccagag
     2881 gcgacggttg cgcaacgtca gttcacgcaa cagcagcgcc accagcaaca gcgccgccag
     2941 ccccgccagg gcctcgctta gacgcaagtg ccccccacca aaggccagtt tggtcattag
     3001 cggcggcatg tcatacatca ccgaggcccg cccgcgatcg atgtcgacca gaaagacgac
     3061 gtagtcgccg ttgtggtcgg tgtcgaaggc gaagggaatt ggttcggtgg tgcggccgta
     3121 ggcatcgatg gcgcgcttgg ggtcgccggc gatgacgtag gactgatagt tgccggagcg
     3181 ggcgatgcgg cgcacttgct ggtagatgcc gccgccgcca agtggctcca gccggccgct
     3241 ggcgtcgtcg acgatccagt cctggaagct gccaatgatg tggaagtccg tggcgctgtc
     3301 gagggcggtg acggcgtagg gaatcgggaa gaagaggcgg tcggcgcgct cattgctgtc
     3361 gaagaggaat gtcaccggtt ggttgggttg ctcggtagtg atccaggcca tcgaggctgg
     3421 gaaggcgcgc gtcgtgtcgg agcaatcgac cacctgccac aggtagtggc caggttgggc
     3481 aatggtgtac tccagcgaga agacagcgtc attggccagc acgtcaccct gggagccgtc
     3541 atcgagcaag cgcggctggg catcgtcggc aaagtcgccc cggacacacc agttgccgct
     3601 atcggccgcg ttgagcgccg cgacgtcggg cgtggatact ggccgggcca gcgaggccgt
     3661 ctctagcgcc aggcgcacga atggattgac aaagtagacc actgccgcga cggccaacag
     3721 gatcagactg gtcgccagca tcgccgtcga tagccggctc ggccggcgga gggattggcg
     3781 cgcggggcgc ggaacgcgtg gtgtgtgcgg ggcgcgtggt gggcgctggg ggcggggggc
     3841 gcgggcgcgc ttggctttgc ggctggcggg cgcgacggct ggctcggcca gttcggcgct
     3901 gccggcggac acgccttctt caagcagatc ctcatcagcc agttctgagt ggaacaaaac
     3961 agcggggagg agttcttcgg gcgcggcctc aggctcagcc cactgaagcc gcggtagttc
     4021 ctcggttagc ggcggcgcag gcggcgcttc ctcgactggg gacactgtct cctccgcgag
     4081 gagctggtgc agccaggcag ggacggccgg ctccggaaac ggcccgcctg cttcaggcac
     4141 gactgcttcg ggcacgactg cttcgggcgc acctgcttcg ggcgcgcctg cttcgggcac
     4201 gcctgcttca ggcgcgactg cttcaggcgc gactgcttca ggcgcgactg cttcgggcgc
     4261 gactgcttcg ggcgcgactg cttcgggcgc gactgcttcg ggcgcgactg
//
LOCUS       DAOSMQ010000263         4302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20517_length_4302_cov_4.619261, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000263 DAOSMQ010000000
VERSION     DAOSMQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20517_length_4302_cov_4.619261"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            455..2275
                     /locus_tag="PK829_09460"
     CDS             455..2275
                     /locus_tag="PK829_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41482.1"
                     /translation="MDEALPNQPLPMLRHYLRPHRGRVALLFLLLGGSIALQLIAPQL
                     LRRFVDVAAGSLVPAGGFLPTSLYAVAALFFVAVLVQKSIYMVTVYLTEDLGWATTNA
                     LRGDLTAHVLRLDMGFHKLRTPGELIERIDGDVGELAEYFSEIIVNLLGNGLLVAGIL
                     VLLFREDWRVGLVGLAYALVVLLIFRTIQARMVTLFGRISQASAALLGFLEEYMTGTE
                     DVVPNGGGSYVMARLFPLLNTQAGLLARTHTLSNVVSATSRFLYALALAATMGLSALA
                     FRAGTMTIGTVFLLMFYVGLLESPIDTVQRELANIQRALASVNRTREFLVLRPEVNDP
                     RAATALPAAPPAVAFAGVAFAYKDRSPATAIAEPAAANHDPVLQDVTFDLAPGRVLGV
                     LGRTGSGKTTLTRLLFRLYDVDAGAITLDGIDLRAVALGELHRHVGLVTQDVQLFAAS
                     VRDNLTLFGHDDPAFDDATIIATLETLGLGPWLRGLPAGLDTVLESGGKGLSAGEAQL
                     LAFARVFLRDPRLVVLDEASSRLDPATEQLLERAIDRLLAGRTGIIIAHRLRTVNRAD
                     DILILDNGRVVEHGPRVALAADPGSRFTQLLQTGLEEVLA"
     gene            2430..>4302
                     /locus_tag="PK829_09465"
     CDS             2430..>4302
                     /locus_tag="PK829_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41483.1"
                     /translation="MDKLSVWKGTWALIRFRPWPFAANLIFGLAFFLLELLPGLISRR
                     FFDELTGSAPATLGISTLLALFVGVHLARTVAGIGYEWAGWVVRTANGVLMHANFMAN
                     ILAKPAARPMPVEPGDAIHRLDRDVGDFADFPTWLPQEVGQAIFFLFAVIIMARINPG
                     ITAVALLPLLAVLFINRIAWRRFLVYDRATRVASSRVTAFLGEILSGVQAVKVAGAEA
                     GALRYFDTLSEARRRAGVRQNTFLTLSFSAGQSMGDIAIAVVVLLAGNAIRSGTMSVG
                     DFALFVSYLTIAVHFPADMGSFISETAQKRVVLDRLQEMHPDAPPASIVARRPLYDKG
                     GAPAVALPARTAADRLERLEVVGLSYLHPASGSRGAGEPGGVEESGQWSVVSDQLEEP
                     ASNGRQSDGPSPLLPRSPAPRRERGGIHNVTFTLPRGSFTVITGRIGSGKTTLLRALL
                     GLLPPGGGEVRWNGAAVADPAAHFTPPRSAYTPQVPRLFSQPLRDNILLGLPDDGRLA
                     DALHTAVMEPDIAALEAGLDTVVGPRGVRLSGGQVQRAAAARMLARDAELLVFDDLSS
                     ALDVETEALLWERLVGAPEERTAGAARPTCLVVSHRRPALRRADNILVLDGGRVVAQG
                     "
BASE COUNT          611 a         1516 c         1423 g          752 t
ORIGIN      
        1 tgtagtccgg ttgattgatg cgctggcagg ctgacagcct gcgctacggt tggtgcgctg
       61 gctgatcctg agtgtagtcc ggttgattga cacggccgcc ccgtctatgc tattctgctg
      121 cccgtcttgc caatcatggc gacaatagga caggaggccc agacccatga cagcttcagc
      181 ccacaagatc attcccgcga cccggttcga ccaggagagc gccgcgaggt agtttcgcgc
      241 ccgcgcctgc ctcgcgcagg cccccgattc ccccgtccgc ggtctcccgc ggctctgccc
      301 gccgcgccct cttcccgcgg gaagtccggc gtgtcgtcag ccgttcgtcc ccgccgggtt
      361 gccggccaat gacggccgtc gttgccggcc gccgttgccg gccgattcgc caccgctctc
      421 gccgtgctct gtctgtttgt tgtggtctgt cactatggat gaagcgcttc caaaccaacc
      481 gctaccgatg ctgcgccact acctgcggcc ccaccgcggg cgcgtagccc tgctgttctt
      541 gctgctgggc ggctccattg ccttgcaact gatcgccccg caactgctgc gccgcttcgt
      601 agacgtggcc gccggcagtc ttgtgccggc cggcggcttc ctgccaacca gcctctatgc
      661 cgtggccgcg ctcttcttcg tcgccgtcct ggtccagaag agcatctaca tggttacggt
      721 ctacctgacc gaagacctgg gctgggcgac gaccaacgcc ctgcgcggcg acctgaccgc
      781 ccacgtcctg cgcctggaca tgggctttca caagctgcgc acgcccggcg aactcatcga
      841 gcgcatcgac ggtgacgtcg gcgagctggc cgagtatttc tcggagatca ttgtcaacct
      901 gctgggcaac ggcctgctgg tggccggcat cctggtgctg ctcttccgcg aggattggcg
      961 cgtggggctg gtcgggctgg cctatgcgct ggtcgtgctg ctgatcttcc ggacgatcca
     1021 ggcgcgcatg gtgaccctgt tcggccgcat cagccaggcc tcggcggcgc tgctgggctt
     1081 cctggaggaa tatatgaccg gcacggaaga tgttgtgccc aacggcggcg gcagctacgt
     1141 gatggcgcgc cttttcccgc tgctcaacac ccaggccggc ctacttgcgc gcacccacac
     1201 actcagcaac gtcgtcagcg cgacgagtcg ttttctctac gcgctggccc tggcggcgac
     1261 gatgggcctg agcgcgctgg ccttccgcgc tggaacgatg accatcggca cggtcttcct
     1321 gctgatgttc tacgtcggcc tgctggagtc gcccatcgac acggtgcagc gggaactggc
     1381 taacatccag cgcgccctgg ccagcgtcaa ccgcacgcgc gagttcctcg tcctgcggcc
     1441 ggaggtgaac gaccccaggg cggcgacggc tctgcccgcc gcgccgccgg cggtggcttt
     1501 tgccggcgtc gccttcgcct acaaggacag gtcgccggcg acggctatcg ccgaaccagc
     1561 cgccgccaac catgatcccg tcctgcaaga cgtcaccttc gacctggccc ccggccgcgt
     1621 cctgggcgtg ctggggcgca ccggcagcgg caaaacgacg ctgacgcggc tgctctttcg
     1681 actctacgac gtcgacgccg gggccatcac cctggacggt atcgacctgc gcgccgtggc
     1741 cctgggcgaa ctgcaccggc acgtcggcct ggtgacgcag gacgtgcagc tcttcgccgc
     1801 cagcgtgcgc gacaacctga ctctcttcgg ccacgacgac ccggccttcg acgacgcgac
     1861 catcatcgct acgctggaga cgttgggcct cggcccgtgg ctgcgcggcc tgccggccgg
     1921 gctggacacg gtgctggaga gcggcggcaa gggcctgtcg gccggggagg cgcaactgct
     1981 ggccttcgcg cgcgtcttcc tgcgcgaccc gcgcctggtg gtgctcgacg aggcctcgtc
     2041 gcggctcgac ccggccacgg agcaactgct ggagcgggcc atcgaccggc tattggccgg
     2101 gcgcactggc atcatcatcg cccaccgcct gcgcaccgtc aaccgggccg acgacatcct
     2161 gattctggac aatggtcgcg tcgtggagca cggcccgcgc gtggccctgg ccgccgaccc
     2221 cggctctcgc tttacccagt tgcttcaaac cggattggag gaagtgctgg cctgatgaaa
     2281 aacagctcac gcaaagaacg ccaagggagc caagagcgct aagaagagtt cttttttgcg
     2341 ccctttactc ccttagcgtc ctttgcgtga gctcttctaa cgaaaacaag agacacgcca
     2401 tgcagagaag agacaaatct gtccaatctg tggataagct atccgtctgg aaaggcacgt
     2461 gggcgctgat ccgcttccgg ccgtggcctt tcgccgccaa cctgatcttc ggcctcgcct
     2521 tcttcctcct ggagttgctc ccggggctga tcagccggcg ctttttcgac gagctgaccg
     2581 gctcggcccc ggcgacgctc ggcatcagca cgctgttggc gttgttcgtg ggcgtccacc
     2641 tggcgcggac ggtcgccggc attggctacg agtgggccgg ttgggtcgtg cgcaccgcca
     2701 acggcgtcct gatgcacgcc aacttcatgg ccaacatcct ggccaagccg gcggcgcggc
     2761 cgatgccggt ggagccgggt gacgctatcc accggctaga ccgcgacgtg ggcgacttcg
     2821 ccgacttccc cacctggctg ccgcaagagg tcggccaagc catcttcttc ctgttcgccg
     2881 tgatcatcat ggcccgcatc aacccgggca tcaccgccgt cgccctgctg ccgctgctgg
     2941 cggtgctgtt catcaatcgc atcgcctggc ggcgcttcct ggtctatgac cgggcgacac
     3001 gcgtcgcgtc aagccgcgtc accgccttcc tgggcgagat tctcagcggc gtgcaggcgg
     3061 tcaaggtcgc cggcgccgaa gccggggcgc tgcgctactt cgacacgctt agcgaggcgc
     3121 ggcgccgagc cggcgtgcgc cagaacacct tcctgacgct gtctttctcc gccgggcaga
     3181 gcatgggcga catcgccatc gccgtggttg tcctgctggc cggcaatgcc atccgctccg
     3241 gaaccatgag cgtgggcgac ttcgccctgt tcgtcagcta cctgaccatt gccgtccact
     3301 tcccggccga catgggcagc ttcatctcgg aaaccgccca gaaacgggtg gtgctcgacc
     3361 gcttgcagga gatgcacccc gacgcgccgc cggcgagtat cgtcgcccgg cggcccctct
     3421 atgacaaggg cggcgcgccg gccgtggccc tgccagccag gacggccgcc gaccggctgg
     3481 agcggctgga ggttgttggg ctgagctatc ttcatcccgc gtcggggagc cggggagctg
     3541 gggagccggg cggagtggaa gagagtggtc agtggtcagt ggtcagtgat cagttggaag
     3601 agccggcaag caacggtcgg caatccgatg gcccatctcc cctgctcccc cgctctcctg
     3661 ctccccggcg agaaagaggc ggcatccaca acgtcacctt cactcttccc cgcggatcgt
     3721 ttacagtgat caccggccgc attggcagcg gcaagacgac gttgctgcgg gcgctgctgg
     3781 ggctgctgcc gccgggcggg ggcgaggtgc gctggaacgg cgcggcggtg gccgacccgg
     3841 cggcccactt cacgccgccg cgctcggcct acacaccgca agtgccgcgc ctgttcagcc
     3901 aaccgctgcg cgacaacatc ctcctgggcc tgcccgacga cgggcggctg gccgacgcgc
     3961 tccacacggc ggtgatggaa cccgacatcg ccgcgctgga ggccgggctg gatacggtcg
     4021 tcgggccgcg cggcgtgcgg ctgtcgggtg ggcaggtgca gcgggcggcc gcggcgcgga
     4081 tgctggcgcg cgacgccgaa ctgctggtct tcgacgacct gagcagcgcc ctggacgtgg
     4141 agacggaggc gctgctgtgg gagcggttgg tgggcgctcc ggaggagcgc acggccggcg
     4201 cggcgcggcc gacgtgcctg gtcgtcagcc accgccgccc ggcgctgcgc cgcgccgaca
     4261 acatcctcgt cctcgacggc ggccgcgtcg tggcccaggg ga
//
LOCUS       DAOSMQ010000264         4293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20570_length_4293_cov_5.309816, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000264 DAOSMQ010000000
VERSION     DAOSMQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20570_length_4293_cov_5.309816"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PK829_09470"
     CDS             <1..379
                     /locus_tag="PK829_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41484.1"
                     /translation="THKDVALAEMRRVLRPGGVLFLIETVEDNPIIHWGRRLYPKWRG
                     DEINALFTFAGLAMMLSDAGFRVSRAGQYSLLFWLWEVLPDLFPAMEKLTPAAVAVEE
                     QLQRFGRQYSAHCYFAATKEAWL"
     gene            385..1662
                     /locus_tag="PK829_09475"
     CDS             385..1662
                     /locus_tag="PK829_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41485.1"
                     /translation="MGALTLRLRTAGDRPAIQGGFMAAAMVVAGGFDYLVNIVAGRQL
                     APTEFFIFVTVTALLQVMVQVTNVIRNVVAYYTAAATVGDEATARVGSVLGRSWRWAW
                     RWGVAATALLAGLSPLLARFLHIPTPWPLWAASLALLLLFLRPVTDGALQGTQHFFGL
                     GAVQMLQSLLRLVFAVGLLWLGARAAGAVVALPLASSVALVLALALLRPYFRASHPAG
                     PPTAISWRYSAYTLLGLLAFAIMANADAIVVRRFFGDVAAEAYAPVVTLGKMNLFIPL
                     GIGLVLFPKATQRHAAGRDPRPVLLLALAATLLPGLLLTVLYALWPGRIVALVFGAAY
                     ADPGRLLALVGLATTLYAGVNIWLNYALSLERRSYMLILVLIVAGQLAAMIFFHARLE
                     NIAWVMLAAGLLANAAGAARLLRPAAPPALASA"
     gene            1696..3543
                     /locus_tag="PK829_09480"
     CDS             1696..3543
                     /locus_tag="PK829_09480"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOU41486.1"
                     /translation="MPNRYLTKSDFKVAQTCPTKLYYRKLGYPTREDGDEYLQALAEG
                     GYLIEALARALFPDGRWVGYRDDVESAVWDTMTALAADPTTLFEATFVSGARMARADI
                     LLKRGRTLELIEIKSCGFDRQANDAQLAAGQRNLFYSPRSPDGLKAAWRPYIEDAAFQ
                     VSILQDVFPDMDIVPYLLMPDTSRPCSIDGLHHRFTLRSVAGYLDAGQRPAADYNGDA
                     RELHRGLFLTRVDVSQEVSRVLPAVRQAAEGYVADLLPALRRRPGTLAVGCRQCEYRV
                     SEGDQRGFHECWGALGDAQPHILDLYHISNLSGRQKPLANELIAAGRVSLFDIPEQRL
                     VRSDGALGEVAQRQRRQIAAARQNREWQAEELAALLAALPYPHYFLDFETCTPAVPRY
                     RGMRPFEVIAYQWACQTVTAPDAPPRHSEWLQPTDTYPNSAFAASLRRQLGDEGAILV
                     WGSHEKTVLKTIQRQMDERGETDTEVYAWLGRTLADDRLVDMCQLTLRHYIHPRMGGS
                     TSLKQVADAVWQSDAAVRARLPQYAQATDDGWASPYAALPPLLVGKRSVSVSEGTGAM
                     LAYFSMMERAAAGATLEAGQWRRLLLQYCQLDTLAMVMVWWHWRGRTAG"
     gene            complement(3540..>4293)
                     /locus_tag="PK829_09485"
     CDS             complement(3540..>4293)
                     /locus_tag="PK829_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41487.1"
                     /translation="RPEPPGDAYGLESLLYDLADRRVVLLMEEFDQLRAADFGADFMT
                     ELRALASVWDYELGFVTASYWDLFRLGNFVGLPVTSPFYNIFHPTAIYLSGLSPAELD
                     ELVRVPARRVGIVAEDEDVAYVRRIAGSLPFFVQATATIWLADKLDNHRGGERDVTAR
                     LVSEMGPYFEQWWRNFSDVERDTLATIAAEKPVERLPYSETEVAAAVLRLRHYGILAA
                     TGGLWIDGLLFQSWLREMVAAAPAAPGPHRRV"
BASE COUNT          649 a         1523 c         1406 g          715 t
ORIGIN      
        1 gacccacaag gatgtcgcgc tggctgaaat gcggcgcgtg ttgcggccgg gcggtgtcct
       61 ctttctcatc gagacggtcg aggacaaccc catcatccac tggggccggc ggctctaccc
      121 caagtggcgc ggcgatgaga tcaacgcgct gttcaccttc gccgggctgg ccatgatgct
      181 cagcgacgcc ggctttcgtg tgtcgcgggc ggggcaatac agcctcctct tctggctatg
      241 ggaagtgctg cccgacctgt tcccggcaat ggagaagctg actccggcgg ccgtggccgt
      301 cgaggagcag ttgcagcgct tcggccggca gtacagcgcc cattgctact tcgcggccac
      361 caaggaggcc tggctctgac cgcaatgggg gcattgacgc tgcgcctgcg aaccgccggc
      421 gaccggccgg ccattcaggg cggcttcatg gccgcggcga tggtcgtcgc tggcggtttc
      481 gactatctgg tcaatatcgt cgccgggcgg caactggccc caacggagtt cttcatcttc
      541 gtcaccgtca ccgccctgct gcaagtcatg gtccaggtca ctaacgtcat ccgcaacgtc
      601 gtcgcctact ataccgctgc ggccaccgtc ggcgacgagg ccacagcgcg ggtgggcagc
      661 gtcctcggcc gcagctggcg ctgggcctgg cgctggggcg tggcggccac ggccctgctg
      721 gccggcctca gccccttgct ggcccgcttc ctccacatcc cgacgccctg gcccctctgg
      781 gcggccagcc tggcgctctt actgctcttc ctgcggccgg tgacggacgg cgcattgcag
      841 ggcacgcagc acttcttcgg tctgggcgcg gtgcagatgc tgcaatcgct gctgcggctg
      901 gtctttgctg ttgggctgct gtggttgggg gcgcgggcag ccggggccgt cgtggccctg
      961 ccgctggcca gcagcgtcgc tctggtgctg gctctggcgc tgctgcggcc ctacttccgc
     1021 gcctcgcacc ccgctgggcc gccgacggcc attagctggc gctactcagc ctacacgctc
     1081 ctcggtctgc tggcctttgc cattatggcc aacgccgatg ccatcgtcgt gcgccgcttc
     1141 tttggcgacg tagccgccga ggcctacgcg cccgtcgtca cgctgggcaa gatgaatctg
     1201 ttcatccccc tgggtatcgg tttggtgctc tttcccaagg ccacgcagcg gcacgccgcc
     1261 gggcgcgacc cgcggccggt gctgctgctg gctctggcgg caacgctgct gcccggcctg
     1321 ctgctgacgg tgctctacgc gctctggccg gggcgcatcg ttgccctggt cttcggcgcg
     1381 gcctacgccg accccggccg gctgctggcg ctggttggcc tggcgacgac gctctatgcc
     1441 ggggtcaata tctggctcaa ctacgcccta tcgctggagc gacgctctta catgctcatc
     1501 ctggtgctca tcgtcgccgg ccagctggcg gccatgatct tcttccacgc ccggctggag
     1561 aatatcgcct gggtcatgtt ggccgccggg ctgctggcca atgctgccgg cgccgcccgc
     1621 ctccttcgac cggccgcccc gccggccctg gcctccgcct gaccgctacg tgacagcctg
     1681 cctatagaac gcatcatgcc caaccgttac ctcactaagt ccgatttcaa agtcgcccaa
     1741 acctgcccaa ccaagctcta ctatcgcaag ctcggctacc cgacgcgcga agatggcgac
     1801 gagtacttac aagcattggc cgaaggcggc tatctcattg aggcgctggc ccgcgcgctc
     1861 ttccccgatg gccgttgggt gggctaccgc gacgacgtgg agtcagccgt ctgggacacg
     1921 atgaccgcgc tggcggccga cccgacgacg ctgttcgagg ccacttttgt cagtggcgcg
     1981 cgcatggctc gcgcagacat cctgctcaaa cgcgggcgga cgctggaact gatcgagatc
     2041 aagtcctgcg gcttcgaccg ccaggccaac gacgcccaac tggccgccgg tcaacgaaac
     2101 ctgttctatt cgccacgctc gcccgacggc ctgaaggcag cgtggcggcc ttatatcgag
     2161 gacgctgcct tccaggtcag catcctgcaa gacgtgttcc ccgatatgga cattgtgccc
     2221 tacctgctga tgcccgacac cagccggccc tgctccatcg acggactgca ccaccgtttc
     2281 acgctgcgct cagtggcggg ctatctggac gccggacagc gccccgcggc cgactacaac
     2341 ggtgacgccc gtgaactgca ccgcggcctc tttctaacgc gcgtcgatgt cagccaggag
     2401 gtcagccgcg tgctgccggc cgtgcgccag gcggctgagg ggtacgtggc cgatctgctg
     2461 cccgcgctgc gccggcggcc gggcacactc gcggtcggtt gccgccagtg cgaataccgc
     2521 gtctctgagg gcgaccagcg cggcttccac gagtgctggg gcgcgttggg cgacgcgcaa
     2581 ccccacatcc tcgacctcta ccatatctcc aacctgagcg ggcgacagaa gccgctggcc
     2641 aacgaactca tcgccgccgg tcgggttagc ctgttcgaca tcccggagca acgcctcgtc
     2701 cggagcgatg gcgcgctggg cgaagtggcc caacgccagc gccggcagat cgccgccgcg
     2761 cgacagaacc gggagtggca ggccgaagag ctggccgcgc tgctggcggc actgccctac
     2821 ccccactact tcctcgactt tgagacctgc acgccagccg taccgcgcta ccggggtatg
     2881 cggccgttcg aggtcatcgc ctaccagtgg gcctgccaga ccgtgaccgc gcccgacgcg
     2941 ccgccgcgcc acagcgagtg gctccaaccg accgacacct atcccaacag cgccttcgcc
     3001 gcgtctctac gccggcaact gggcgacgag ggcgcaattc tcgtctgggg cagccacgag
     3061 aagacggtgc tgaaaaccat ccagcgccag atggacgagc ggggcgagac ggacacggag
     3121 gtctatgcct ggctggggcg cacgctggcg gacgaccgtc tggtagacat gtgccagttg
     3181 acgctccggc actacatcca cccgcgcatg ggcggcagca cgtcgctcaa gcaagtggcc
     3241 gatgctgtct ggcaaagcga cgccgccgtg cgcgcccggc tgccgcaata cgcccaggcg
     3301 acggacgacg gctgggccag cccctacgcc gcgctaccgc ctctgctggt gggcaagcgg
     3361 tcggtgtcgg tctctgaggg cacaggggcg atgctggcct acttcagcat gatggagcgc
     3421 gccgccgccg gggccacgct ggaagcaggg caatggcgcc ggctgttgct gcaatactgc
     3481 cagctggata cgctggcaat ggtgatggtc tggtggcact ggcgcggccg gacagccggc
     3541 tagacgcgcc ggtgtggccc gggcgcggcc ggggccgcgg cgaccatctc ccgcagccag
     3601 ctctggaaca gtagcccatc gatccacagc ccgcccgtcg ccgccagaat gccatagtgc
     3661 cgcaggcgca ggacggccgc ggcaacctcc gtctcgctgt agggcaggcg ctcgaccggc
     3721 ttctccgcgg caatggtcgc cagcgtgtcg cgctccacgt cgctgaagtt gcgccaccac
     3781 tgctcgaaat acggccccat ctccgacacc agccgcgccg tcacgtcgcg ctcgccaccg
     3841 cggtggttgt cgagcttgtc ggccagccag atggtcgccg tggcctgcac gaagaagggc
     3901 agcgagccgg caatgcggcg cacgtaggcc acgtcctcgt cctcagcgac gatgcccacg
     3961 cgccgcgccg gcacgcgcac caactcgtcc aactccgccg ggctgaggcc ggacaggtag
     4021 atcgctgtgg gatggaagat gttgtagaac ggcgacgtga ccggcaggcc aacgaagttg
     4081 cccagccgga agaggtccca gtaggaggcg gtgacaaagc ccagctcgta gtcccagacg
     4141 ctggccaggg cgcgcaactc ggtcatgaag tcggccccga agtcggccgc gcgcaactgg
     4201 tcaaactctt ccatcagtag caccacgcgc cggtcggcta ggtcgtagag cagcgattcc
     4261 aggccgtaag cgtcgccggg cggctctggc cgg
//
LOCUS       DAOSMQ010000265         4287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20604_length_4287_cov_3.588374, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000265 DAOSMQ010000000
VERSION     DAOSMQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20604_length_4287_cov_3.588374"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="PK829_09490"
     CDS             <1..1339
                     /locus_tag="PK829_09490"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOU41488.1"
                     /translation="VALAVAGWWLSRPGQRAFLRPVSPSAAYPASGEPPATRVYVRDD
                     WLTLVVVAGTILLLTLFRYTGDFRLTASRPPSFLFVNLTMVEVGLLFAALALVARRLP
                     QRAVLTGAIVAVVALFVALKWPPATTALAGAWRGWTGQDVALAAPADLAWLGFSYVAF
                     RLIHTLRDRQAGILPDLSLRAYLSYVLFAPALVAGPIDRAERFAGDYTALPGLRGLDA
                     ARWGLGLWRIGEGLLKKFVLADLLAGGLSLTPALAGQTTSPAALWALLLGYGLRLYLD
                     FGGYTDIAIGLGILFGIRLPENFDRPYTRTSLTAFWQSWHITLSNWARFYVFTPLSRR
                     LLRGQRRLPTAVIVLLGHLATMIVIGLWHGISWTFLIWGVWHAVGLFVHKQWSDRTRR
                     WYRGVQGRPWPRRAWAAAGWALTFVYVMLGWVWFLMPDVGGAVGVFARLLGVR"
     gene            1571..2779
                     /locus_tag="PK829_09495"
     CDS             1571..2779
                     /locus_tag="PK829_09495"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU41489.1"
                     /translation="MQLITWLEDRIAQPDDDEFTRRQKAFSFIPAGSTVVLASSWAAI
                     FYAIGWRPVAFIYLAYSMLCLLVVIGALRLPRYALVFGTTMSIAGIFANLLAHLVTGG
                     FDSGLWYLLWVIILPLAAYLSGSYRGLSLLVLAVALASLGVALAAEAQLAPAAQAPHW
                     LLILYNGFVTVTATVMLFLWGVYILQELNAARRRADDLLLNILPAPIAAQLKRNPGAI
                     AQAYDAVTVLFADIVGFTPLSATADPVVVVNFLNDLFSDFDELAARHGLEKIKTIGDA
                     YMIVGGVPTPRPDHCEAVAAFAVEMLAAVRRRPAWNGQPVRLRIGINTGPVVAGVIGR
                     EKFIYDLWGDVVNTASRMEAHGQAGVIQVTEAVRDCLADCFEFEAQPPAYIKGKGQMV
                     TYFLKVPTPA"
     gene            complement(2757..3800)
                     /locus_tag="PK829_09500"
     CDS             complement(2757..3800)
                     /locus_tag="PK829_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41490.1"
                     /translation="MRHHVLLFLLATLLLLPACAPASGAPAPTPPAGTPAPDGPATRP
                     APVVAQNSGPTPAPATLTAVTALPLTPAAPAATAAPSATPSPTPEPSPTPLGPQTING
                     LPLSAIAPLPPEVVANVRAIYATGQKLGRDPNAFSKLGDSTLLNPHFLGPFDSGDYNL
                     GDFGHLQPTIDRFAGSFERHGVAARHGLHSWTVFDPMWADPDWCEANEDVLACEIRLQ
                     NPSFIFIRLGSNDAGSPSGFRFNVKKVIEFAIANGVVPIIGTKADRFEGSNENNDILR
                     ALAGEYHVPLWDFDRLADTLPGRGLDTDGVHLIIDDLPHDYTDPAAFQRGHAMQDLSA
                     LIVLDQLRRALEP"
     gene            4132..>4287
                     /locus_tag="PK829_09505"
     CDS             4132..>4287
                     /locus_tag="PK829_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOU41491.1"
                     /translation="MKIGVVGCGFVGSTAAYAMVMNGVGRELVMVDLNRKRAEAEAAD
                     ILHAVPFA"
BASE COUNT          591 a         1338 c         1568 g          790 t
ORIGIN      
        1 ggtggccctg gccgtggccg gctggtggct gtcgcggccg gggcaacgcg cgttcctgcg
       61 gcccgtctcg ccgtccgccg cctatccggc cagcggcgaa ccgccggcga cgcgcgtcta
      121 cgtgcgggac gactggctga cgctggtcgt cgtggccgga acgatcctgc tgctgacgct
      181 cttccgctac acgggcgact tccgcctgac ggcctcgcgg ccgccgtcgt ttctcttcgt
      241 caacctgaca atggtcgaag tggggctgct gttcgcggcg ttggcccttg ttgctcgccg
      301 cctgccgcaa cgcgccgtgc tgaccggagc catcgttgcc gtcgtggccc tcttcgtggc
      361 cctgaagtgg ccgccggcaa cgacggcgct ggccggggcg tggcgcggct ggacggggca
      421 ggacgtggcc ctggccgcgc cggccgacct ggcctggcta ggcttctcct acgtcgcctt
      481 tcgcctgatc cacaccctgc gcgaccggca agctggcatc ctgcccgacc tgtcactgcg
      541 cgcctatctg agctacgtcc tcttcgcccc ggccctggtc gccgggccga ttgaccgggc
      601 cgaacgcttt gccggcgact acaccgccct gccggggctg cgcgggctgg acgcggcccg
      661 ttgggggctg ggcctgtggc gcatcggcga ggggctgctg aagaagttcg tcctggccga
      721 cttgctggcg ggcgggctgt cgctgacgcc ggcgctggcc gggcagacga ccagcccggc
      781 ggcgctgtgg gcgctgctgc tgggctatgg cctgcgcctc tatctcgact tcggcggcta
      841 caccgacatc gccatcggcc tgggcattct cttcggcatc cgcctgccgg agaacttcga
      901 ccggccctat acgcgcacca gcctgaccgc cttctggcag agctggcaca tcaccctcag
      961 caactgggcg cgcttctacg tcttcacgcc gctatcgcgc cgcctgctgc gcggccagcg
     1021 ccggctgcca acagccgtca tcgtcctgct cggccatctg gcgaccatga tcgtcattgg
     1081 cctgtggcat ggcattagct ggacgttcct gatctggggc gtctggcatg ccgtggggct
     1141 attcgtccac aagcagtgga gcgaccggac gcggcgctgg tatcgcggcg tgcaggggcg
     1201 gccgtggccg cggcgggcat gggccgcggc cggctgggcg ctgacgttcg tctacgtgat
     1261 gctgggctgg gtgtggttcc tgatgccgga tgtgggcggg gctgtgggcg tgtttgcgcg
     1321 gctgttgggc gttcgttaga aaagcaggtg acaaagaggt taacgcatgg atcatgagcg
     1381 aggacgggcc ggcgctggcc gcggccgggc tcgctgtggc ggttgagcag ttgtccggct
     1441 gtgggttggc gctggccatt cgcccggcaa cgtgatactg tcggaacgtt aggcgagcgg
     1501 ttggcgtgcg gctgtcatgt cgcctggcgg gatgccgtca gggcggcctg gcagggaggc
     1561 attggcgaat atgcaactca tcacctggct ggaagaccgg atcgcgcagc cggatgacga
     1621 cgagtttacg cggcggcaaa aggctttctc cttcatcccg gccggttcga cggttgtgct
     1681 ggcctcaagt tgggcggcca ttttctacgc gatcggctgg cggccggtgg cgttcatcta
     1741 cctggcctac tccatgctat gtctcctggt ggtcattggc gcgctgcggc tgccccgcta
     1801 tgccctcgtc tttggcacaa cgatgtcgat tgccggcatc ttcgccaacc tgctggccca
     1861 cctggtgact ggcggcttcg acagcggcct gtggtatctg ctgtgggtca tcatcctgcc
     1921 gctggcggcc tatctgagcg ggagctaccg ggggctgagc ttgctggtgc tggctgtggc
     1981 cctggccagt ctgggtgtgg ccctggccgc cgaagcgcaa ctggccccgg cggcgcaggc
     2041 gccccactgg ctgctcatcc tctacaatgg ctttgtcacc gtcacggcga cggtcatgct
     2101 ctttctttgg ggcgtctaca tcttgcaaga gctaaacgcc gcccgccgcc gcgccgacga
     2161 tctgctgctc aacatcctgc ccgcgcccat cgccgcgcag ctgaagcgca accccggcgc
     2221 gattgcccag gcttatgacg ccgtgaccgt gctcttcgcc gatatcgtcg gcttcacccc
     2281 cctgagcgcc acggccgatc cggtcgtcgt cgtcaacttc ctcaacgacc tcttcagcga
     2341 cttcgacgaa ctggccgcgc gtcatggact ggagaagatc aagaccatcg gcgacgccta
     2401 catgatcgtc ggtggggtgc caacgccgcg gcccgaccac tgcgaggccg tggccgcctt
     2461 cgccgtggag atgctggcgg ctgtgcggcg gcggccggcg tggaatggcc agcccgtgcg
     2521 cctgcgcatc ggcatcaaca ccgggccggt ggtggcgggg gtcatcggcc gcgagaagtt
     2581 catctatgac ctctggggcg acgtggtcaa caccgccagt cgcatggagg cgcatgggca
     2641 agcgggtgtc attcaggtca ccgaagcagt acgcgactgc ctggccgact gcttcgagtt
     2701 tgaagcccaa ccgccggcct acatcaaggg caagggccag atggtgacct acttcctcaa
     2761 ggttccaacg cccgcctgag ctggtcgagc acaatcagcg ccgaaaggtc ctgcatggcg
     2821 tggccgcgct ggaaggcggc cgggtcggtg tagtcgtggg gcagatcgtc gatgatgagg
     2881 tggacgccgt cggtgtccag gccgcggccg ggcagggtat cggctaggcg gtcgaagtcc
     2941 cacaggggca cgtggtactc gccggccagg gcgcgcagga tatcgttgtt ctcgttgctg
     3001 ccctcgaaac ggtcggcctt cgtgccgatg atgggcacaa ccccgttggc aatggcgaac
     3061 tcgatgacct tcttgacgtt gaagcggaag ccgctgggcg agccggcgtc gttggaaccc
     3121 aggcgaatga aaatgaagct ggggttttgc aggcggattt cgcaggccag cacatcctca
     3181 ttggcctcgc accagtcggg atcggcccac atggggtcga agaccgtcca ggagtgcagg
     3241 ccgtggcggg cggcgacgcc gtggcgctcg aaggagccgg cgaagcggtc gatggtcggc
     3301 tggaggtggc cgaagtcgcc caggttatag tcgccgctgt cgaaggggcc gaggaagtgg
     3361 gggttcagca gggtgctgtc gcccagcttg gagaaggcgt tggggtcgcg gccgagtttc
     3421 tgtccagtgg cgtagatggc gcggacgttg gcgacgactt cgggcggcaa gggggcgatg
     3481 gcgctgaggg gcaggccgtt gatcgtctgt gggccgagcg gcgtgggcga tggctccggg
     3541 gtgggcgagg gcgtagctga gggggcggcc gtggccgcgg gcgcggccgg cgtcaggggc
     3601 agcgcggtga cggcggtcag ggtggccggg gcgggcgtgg ggccgctgtt ctgggcgaca
     3661 acgggggccg ggcgcgtggc cgggccgtcg ggcgcgggtg tgccggccgg tggcgtgggc
     3721 gcgggcgcgc cgcttgccgg cgcgcaggcg ggcagcagca gcaaggtcgc cagcaagaag
     3781 agaaggacgt ggtgtcgcat cggggtatgt gcccgcaccc gacgaagcag actttgcttc
     3841 gggcacattc tctaaagaaa gcgcccggcg ctatgggagc aaggcgctac ggtaaggctg
     3901 tctcgcgcgt gggagccacg aaacggcctg acgaccgctg gcaggccaat catggccctg
     3961 gacttgccgc ctggccgctc tttgcgccgg tcgttcacag atgaccgccg gcggacgttg
     4021 gcgctattcg ttggtgcgat gttcatgttc ggttagaatc tcccacagac aagtcaagtc
     4081 tactcagttt ccccgtcgag gcaatgtggg gcgtctacac aggagcaacg gatgaagatt
     4141 ggagttgtgg gctgcggctt tgttggctca acggccgcct atgcgatggt gatgaatggc
     4201 gtcgggcgcg agctggtgat ggtggacctg aatcgcaaac gcgccgaggc cgaggcggcc
     4261 gacattctcc acgctgtgcc ctttgct
//
LOCUS       DAOSMQ010000266         4286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20608_length_4286_cov_5.438194, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000266 DAOSMQ010000000
VERSION     DAOSMQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20608_length_4286_cov_5.438194"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PK829_09510"
     CDS             complement(<1..491)
                     /locus_tag="PK829_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOU41492.1"
                     /translation="MHIGLQIPSFKYPGGTAAIRPKLKEIVTTAEEAGFYSLWVMDHF
                     YQIKGLFGEAYTDPMLEAYTTLGYFAGLTEKVYLGVLVTGVIYRHPSVLLKMVNTLDI
                     LAGGRTYLGIGAAWYENEARGYGIPYPSTSVRFEQLEDNLQLAKALWQSDETAFQGKH
                     FSAP"
     gene            complement(556..2256)
                     /locus_tag="PK829_09515"
     CDS             complement(556..2256)
                     /locus_tag="PK829_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOU41493.1"
                     /translation="MAIASTQGLTAIATATVATATVATATVATATVATATVATATVAT
                     ATVATATVATATGRVAGGIDARSGVYAFKGVPFAAPPVGERRWRPPQPPPPWAGVRPA
                     DRFGPRPMQLPAFGDMNFRCGEMSEDCLTLNVWTPDPSPAARLPVLVYFYGGGFIAGD
                     GSEPRYDGGRLAGRGLVVVTVNYRLNVFGFLAHPELSAESPHGRSGNQGFLDQRAALG
                     WVRDNIAAFGGDPGRVTIAGESAGSISANAHMVSPLSAGLFAAVIGASGGLRSLPPLA
                     PEAAEQVGLAFGQRIDAPSLAALRAAPADRLLAATRGLAAHDFRGVVDGHFFPAEPLA
                     AATGWPAARVPLMIGWNSAEMDYRVLLGNREPTPRHYAAAVRALYGEQAGEALRLYPG
                     VTTAQVRQSATDLASDRFIVYSAWKWADDQRAAGAPLFRYLYAHPRPPMAPDMAGAVP
                     GLAGGVIDAAQANAAIALPPPAEGAVHSADIEYALGNLDTNHVYAWTAADEQVSALMQ
                     GYYANFIMTGDPNGPGLPVWPRADGRPEMQYMVWDVAPRVEVDRQRERYQFLDRFYRS
                     "
     gene            complement(2273..2677)
                     /locus_tag="PK829_09520"
     CDS             complement(2273..2677)
                     /locus_tag="PK829_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU41494.1"
                     /translation="MEIKKNGTQTSGKGPAEWFTGAVRIDPLHNAPAPARVGCAAVTF
                     EPGARTAWHSHPLGQTLVVIAGCGRAQRWGGPVEEIKPGDVVWIAPGEKHWHGAAPAT
                     GMTHIAIQESLDGGTAQWMEHVSDEQYLGQAA"
     gene            complement(2690..3658)
                     /locus_tag="PK829_09525"
     CDS             complement(2690..3658)
                     /locus_tag="PK829_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU41495.1"
                     /translation="MALPTRTLGQSGLEVSALGLGCMRMSFGDTPTDKDEMITFLHQA
                     VERGVTFFDTAEVYGPFTNEELLGEALSPFKDQVVIATKFGFKHGENGPSPRTGVDSR
                     PEQIRRVAEASLRRLRVEAIDLFYQHRPDPDVPMEDVAGAVKELIQAGKVKHFGLSES
                     NADQIRRAHAVQPVTALQSEYSIWWTEIEPEILPTVEELGIGLVPYSPLGRGYLTGKV
                     DESTTFDSTDIRNRNPRFTQEAIRANRAVVELLERIGAQHNATPAQIALAWLLAQKPW
                     VVPIPGTRSLRRLDENLGALDVHLTANDLSDIKEAMSHITVVGHRY"
     gene            complement(3677..4090)
                     /locus_tag="PK829_09530"
     CDS             complement(3677..4090)
                     /locus_tag="PK829_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU41496.1"
                     /translation="MKISHVSEQWGIPVDTLRYYERIGLLPTVNRSESGIRDYSELDL
                     RRVEFIKCMRGAGLPIEVLARYMDLVQQGDSTIEARKDILIRQRAQLAARMAEMQKTL
                     DLLDHKIEVYEQKILEKEQVIHQLQMDEWESVQGI"
BASE COUNT          689 a         1447 c         1368 g          782 t
ORIGIN      
        1 ggcgcggaga agtgcttgcc ctgaaaggcc gtctcgtcac tctgccagag cgccttggcc
       61 agctggagat tatcctccag ttgctcaaag cgcactgagg tggaggggta ggggatgcca
      121 tagcctctgg cctcgttctc ataccaggcc gcgccaatgc caaggtaggt gcgaccgccg
      181 gccagaatat ccagcgtgtt gaccatcttc agcagcaccg acggatggcg atagatgaca
      241 cccgtcacca gcacgccgag atagaccttt tcggtcagcc cggcgaaata gccgagtgtc
      301 gtataggcct ccagcatcgg gtcggtatag gcttcgccga acaacccctt gatttgatag
      361 aagtgatcca tcacccagag gctgtagaac ccggcctctt cggccgtcgt gacgatctcc
      421 ttcagcttgg gccggatggc cgccgtgccg ccgggatact tgaaagaggg gatttgcagt
      481 ccaatgtgca tgatcgatct ccttattgtc atcgcttggc caacaatgac ctgcctgtgc
      541 ttccagaaag tcttctcatg accggtagaa ccggtcgaga aactgatagc gttcgcgctg
      601 ccggtctact tccacccgcg gggctacgtc ccagaccata tactgcatct ccggcctccc
      661 gtcggcgcgc ggccagacgg gcagccccgg cccgttgggg tcgccggtca tgatgaagtt
      721 ggcatagtag ccctgcatca gcgccgacac ctgctcatct gcggccgtcc aggcatagac
      781 gtggttggtg tccaggttgc ccagcgcgta ctcgatgtcg gccgaatgca ccgcgccctc
      841 ggccggcggt ggcagggcga tggcggcgtt cgcctgcgcc gcgtcaatta ctccaccggc
      901 cagccccggc accgccccgg ccatgtccgg cgccatcggc ggtcgcgggt gggcatacag
      961 atagcgaaac agcggcgcgc cggcggcgcg ctggtcgtcg gcccacttcc aggcgctgta
     1021 gacgataaac cggtcgctgg ccagatccgt ggccgactgc cgcacctgcg cggtcgtcac
     1081 gccggggtag agccgcaggg cttcgccggc ctgctcgcca tacagggcgc gcacggccgc
     1141 ggcgtagtgg cgcggcgtag gctcgcggtt gcccagcaac acgcggtagt ccatctcggc
     1201 cgagttccag ccgatcatga gcggcacgcg ggcggccggc cagccagttg cggcggccag
     1261 cggctcggcg ggaaagaagt ggccgtcgac cacaccccgg aagtcgtggg cggccagccc
     1321 tctggtggcc gccaacagcc tatcggcggg cgcggcccgc agcgccgcca gcgacggcgc
     1381 gtcgatccgt tgcccgaaag ccagcccgac ctgttcggcg gcctccggtg ccagcggcgg
     1441 cagcgaacgc aacccgccgc tggcgccaat gaccgccgcg aacagcccgg ccgatagtgg
     1501 cgagaccatg tgggcgttgg ccgagataga cccggccgac tcgccggcga tggtcacgcg
     1561 gccggggtcg ccgccaaagg cggcgatatt gtcgcgcacc cagcccagcg ccgcgcgctg
     1621 gtccagaaag ccctggttgc cggagcggcc gtggggggat tcggcgctca gttcgggatg
     1681 ggccagaaag ccgaacacat tcagccggta gttgaccgtc acgaccacca gcccgcgccc
     1741 ggccagccgg ccgccgtcgt agcgcggctc ggaaccgtca ccggcgatga agccgccgcc
     1801 gtagaagtag accagcacgg gcagccgcgc cgccggggac gggtcgggcg tccagacgtt
     1861 gagagtgagg caatcttcgc tcatctcgcc acagcggaag ttcatgtcgc caaacgcggg
     1921 caactgcatc ggccgcgggc caaagcgatc ggccggacgc acacccgccc acggcggcgg
     1981 cggttgcggc ggccgccagc ggcgctcgcc gaccggcggc gcggcaaagg gcacgccctt
     2041 gaaggcgtag acgcccgacc gggcatcgat cccaccggcc acgcggccgg tggccgtcgc
     2101 gacggttgcc gtcgcgacgg ttgccgtcgc gacagttgct gtcgcgacag ttgccgtcgc
     2161 gacagttgcc gtcgcgacag ttgccgtcgc gacagttgcc gtcgcgacag ttgctgtcgc
     2221 gatggcggtc aggccctgag tggaggcaat ggccatctcg ttcggctccc gcctaggccg
     2281 cctggccgag gtactgttcg tcgctgacat gttccatcca ctgcgccgtc cctccgtcca
     2341 gggactcctg aatggcgata tgggtcatgc ccgtcgccgg cgcggccccg tgccagtgct
     2401 tctcgcctgg cgcaatccag accacatcgc ccggcttgat ctcctcgacc ggcccacccc
     2461 agcgctgcgc ccggccgcag ccggcaatca ccaccagcgt ctggcccagc gggtggctgt
     2521 gccatgccgt ccgcgcgccc ggctcgaacg tcaccgcggc gcagcccacc cgcgccggcg
     2581 ccggcgcatt gtggaggggg tcgatgcgca ccgcgccggt gaaccactcc gccggccctt
     2641 tgccggacgt ttgtgtacca ttcttcttga tttccattct cgcacctctc taataacgat
     2701 ggccgacgac ggtgatgtgg gacatggcct ctttgatgtc gctaagatcg ttggccgtca
     2761 agtgaacgtc cagcgcgccg aggttctcgt ccagccggcg gaggctgcgg gttcccggaa
     2821 tgggcacgac ccacggcttc tgggccagca gccaggccag ggcgatctgg gccggcgtgg
     2881 cgttgtgctg cgcgccgatc cgctccagca actcgaccac ggcccggttg gccctgatcg
     2941 cttcctgggt gaagcggggg ttgcggttgc gaatgtcggt gctgtcgaaa gtggtgctct
     3001 cgtccacctt gccggtcagg tagccgcggc cgagcgggct atagggcacc aggccgatgc
     3061 ccagttcctc taccgtgggt aggatttcgg gttcgatctc cgtccaccag atcgagtact
     3121 cgctctgcag agcggtgacg ggttgcaccg cgtgggcgcg gcgaatctgg tcggcgttgg
     3181 attcggacag tccgaagtgc ttgaccttac ccgcctggat cagttccttc actgctccgg
     3241 ccacgtcttc catgggcaca tcgggatcgg gccggtgctg gtagaagagg tcaatggcct
     3301 cgacgcgcag ccgccggagt gatgcctcgg ccacgcgccg gatttgttcg gggcggctat
     3361 ccacaccggt gcgggggctt ggcccgttct cgccatgttt gaagccgaac ttggtggcga
     3421 tgaccacctg gtccttaaac ggcgacagcg cttcgcccag cagttcctcg ttggtgaagg
     3481 ggccatagac ttcggccgtg tcaaagaagg tgacgccccg ctccaccgcc tggtggagaa
     3541 aggtgatcat ctcgtctttg tccgtgggcg tgtcgccgaa gctcatgcgc atgcagccca
     3601 gccccagcgc cgaaacctcc aggccgctct gacccagtgt gcgtgttggt agtgccatgt
     3661 ctcgtgttct cctatttcaa atgccttgta cgctttccca ctcgtccatt tgcagttgat
     3721 gaatcacctg ctccttctcc aagatcttct gctcatacac ttcgatcttg tggtccaaca
     3781 ggtcgagcgt tttctgcatt tcggccattc gggccgcaag ctgcgcgcgt tggcggatca
     3841 ggatgtcctt gcgggcttca atcgtgctgt cgccctgctg caccagatcc atgtacctgg
     3901 ccagcacctc tatcggcagc ccggccccgc gcatgcactt gataaactca acccgcctca
     3961 agtccagttc gctgtaatcg cggatgccgc tttcactgcg gttgacagtt ggcagcagac
     4021 cgatgcgctc atagtagcgc agcgtatcca ctgggatgcc ccactgttcg ctcacatgcg
     4081 aaatcttcat ctctcaacct ccgttgaaga gtgtaacacc tggagttaac tctaagtcaa
     4141 gccccaattt ataccagttt ttcgttcgct gtcaggactg gattaccgcc accgtcgtcg
     4201 ccgcggcgcg ttcgccgcta ccggcccacg acgctacaat atcggcccta ccgttgcccg
     4261 cgcggacccg gcggccaatt gagcga
//
LOCUS       DAOSMQ010000267         4274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20680_length_4274_cov_4.254563, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000267 DAOSMQ010000000
VERSION     DAOSMQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20680_length_4274_cov_4.254563"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2256)
                     /locus_tag="PK829_09535"
     CDS             complement(<1..2256)
                     /locus_tag="PK829_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HOU41497.1"
                     /translation="MPAQQLQHNYEELLDRLTNDFALWELDDLCTKLAIPSGDLVLGP
                     TQHDRAASLLRLIDASHRHIDLYRQLREDRPDDQWPRHFLLPTSQTAAVYMNLPDAGR
                     LPELTALTVRSAPYLLTRIGREAEYRALVATLQETVRTRHGRTVCLLGHTGCGRRALM
                     ADVQKSALSLGYRLATIRFEDMEEAQPAHVRVHWGDLAGRAARQFVAETFPRTAKETG
                     GEWADLMRQLADEGTFAYVGQSSAADDPRALNKLIRATGNRDPLLVCVENLDAAQNLW
                     IDLLHDLALQEIPRDLPVVLLIGMDAPQPLAELRGPRHSERTRLAQKLVRDGVADAFF
                     LGPITAADIRAWTGPAGAELIERLAHLSGFDPKVVETLWLEWKTRGVAVPEPGGRWVV
                     AAGARQWVAGDMRDHALALLSAALEGYEDDMPYTAAQVEHILACAALEGEVFHAEAVA
                     RVLELDADELIDFFDTYLTAGEPDNVIDEAEDAVEEDDEEDTAADGWPSEPLLEEAGF
                     YKMALQVYVNGQPTAQYLHLYRFARPYLHLVWERYGPPEAKRKAWPLALANALEALYD
                     PFAMRIAGKLIRLFAAAGADSRAAQYHRLRSGRLTLAQLRWHLDALLAEPTEDKYALS
                     RLFEAAIPFLNRWAREQPGEWPVGLDYALQVASRAEALDDEENQAEAWYYAAWMHYNG
                     AGYAAGVGLARRALAIQERVCGENHLATAASLSILGMLLQAQSDLAGARPLFERALAI
                     YERVLGPEHPQT"
     gene            complement(2263..3372)
                     /locus_tag="PK829_09540"
     CDS             complement(2263..3372)
                     /locus_tag="PK829_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOU41498.1"
                     /translation="MLRLEGPEIIELRDILMRALSLQELEDVLLRLNTSVSRIIIGRN
                     EPEIYREVVVHFNRRTIVDQLVVVAHTVNPTDPNLYLFMQGHGLAMEFPAEATDLPGI
                     EAIIRKHLGTLDARTWIERAAAAENRVCLISYGGQPRGTGFLVGPEAVLTNYHVVRNV
                     VEGADPAGLGFRFDFMFLPDGALGGAGAPLGPVLPDWLIDHSPPYPSDDPLMHPKTPI
                     NPRTPPDVPPDHLDYALLRVAGRPGDAVVGAKATPDGRGQPRGWLQLRATPHDFAAAH
                     ALLVLHHPDGQPLQLSIDTDSFAAVNTSRTRIYHRSNTDYGSSGSPVFDLGWNLVALH
                     QGRGTVNGELLNRAIPVSAIHALLAQHEKLGALGG"
     gene            complement(3639..4220)
                     /locus_tag="PK829_09545"
     CDS             complement(3639..4220)
                     /locus_tag="PK829_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41499.1"
                     /translation="MSSRSGSSLRGGLLLFAAVALFLITAGFAAGRLAAQPAARPTVR
                     ATVLELSCTGIDDVSGTYAKIADVGTFDVQAAESLVELQFNGRLYAGSTDGTGLQFEL
                     RVDDAATSLGRIRAVLKASEIGSGVGRNATMSGFYEGLAAGTHTVSVWARTANGSDSD
                     SVMYDPGCWSSDVVNIKEYLPFGVVSLPAILRN"
BASE COUNT          702 a         1410 c         1451 g          711 t
ORIGIN      
        1 ggtttgggga tgctccgggc cgaggacgcg ctcgtagatg gccagggcgc gctcgaagag
       61 cggccgcgcc ccggccaggt cgctctgcgc ttgcagcagc atgcccagaa tgctgaggct
      121 tgccgccgta gcaagatggt tctcgccaca cacgcgctcc tggatcgcca gagccctccg
      181 ggccagaccg acgcccgccg cgtagcccgc gccgttgtag tgcatccagg cggcgtagta
      241 ccacgcttcg gcctggttct cctcgtcgtc cagcgcttcc gcccgggagg ccacctgcag
      301 cgcgtagtcc agtcccaccg gccactcgcc cggctgctcc ctggcccagc gattcagaaa
      361 ggggatggcc gcctcaaaca gccgcgacag cgcgtacttg tcctcggtcg gctcagccag
      421 cagggcgtcg aggtgccagc gcagttgcgc caacgtcagg cggccgctgc gcaggcggtg
      481 gtattgcgcc gcgcgactgt cggcgccggc ggccgcgaac agacggatca gcttgccggc
      541 gatgcgcatg gcgaaggggt cgtagagcgc ctccagggcg ttggccaggg ccagtggcca
      601 tgccttgcgc ttcgcctccg gagggccata gcgctcccag acgagatgca gatagggccg
      661 ggcgaagcga tacaggtgga ggtactgtgc cgttggctgg ccattgacgt acacctggag
      721 cgccatcttg tagaagccgg cttcctccag cagcggttca gacggccacc catcggcggc
      781 cgtgtcttcc tcgtcgtcct cttcaaccgc gtcctcagcc tcgtcgataa cattgtccgg
      841 ctcgccggca gtcaggtagg tatcgaagaa gtcgatcagt tcgtccgcgt ccaactccag
      901 gacgcgggcg acggcttcgg cgtggaagac ctccccttcc agcgccgcgc aggcgaggat
      961 gtgctccacc tgcgcggcgg tatagggcat gtcgtcctca tagccctcca gcgcggccga
     1021 caggagcgcc agcgcgtggt cgcgcatgtc gccggccacc cattggcgcg cccccgcggc
     1081 cacgacccac cggccacccg gttccggcac ggccacgccg cgcgtcttcc actccagcca
     1141 cagcgtttcc accaccttgg ggtcgaagcc gctcaagtgc gccagccgct cgatcagttc
     1201 cgcgccggcc gggccggtcc aggcgcggat gtcggccgcg gtgatcgggc cgaggaaaaa
     1261 ggcatcggcg acgccatcgc gaacgagctt ctgcgccagg cgcgtgcgct cgctgtggcg
     1321 cgggccgcgc agttcggcca ggggctgcgg tgcgtccatg ccaatgagca gtaccaccgg
     1381 caggtcgcgc ggtatttcct gcaaggcgag gtcatgaagg aggtcgatcc acaggttctg
     1441 ggcggcgtcg agattttcca cgcagacaag cagtgggtcg cggttgccgg tggcgcggat
     1501 cagcttgttg agcgcccgcg ggtcgtcggc cgcgctgctc tggcccacat aggcaaacgt
     1561 gccctcgtcg gccagttgcc gcatcaggtc ggcccattca ccgccggttt ccttcgccgt
     1621 gcgggggaac gtctcggcga cgaactgtct ggcggcgcga ccggccaggt cgccccagtg
     1681 gacgcgcacg tgggctggct gcgcctcctc catgtcctca aagcgaatcg tcgccagacg
     1741 atagccaagc gaaagggccg acttctggac gtcggccatc agggcgcgac ggccgcagcc
     1801 ggtatgcccc agcaggcaga cggtgcggcc gtggcgagtt ctcaccgtct cctgaagtgt
     1861 ggcgaccagc gcgcggtact ccgcttcgcg gccgatgcgc gtcagcaggt agggcgcgct
     1921 gcgaaccgtc agcgcggtca gttcgggcag gcggccggca tccggcaggt tcatatagac
     1981 ggccgcggtc tgggacgttg gcaggaggaa gtggcgcggc cactggtcat ccgggcggtc
     2041 ttcgcgcagt tgcctgtaga ggtcgatgtg gcggtggctg gcgtcgatca ggcggaggag
     2101 gctggcggcg cggtcgtgct gcgtgggccc aagtacgaga tcgccggacg ggatggccag
     2161 cttagtgcaa agatcatcca gttcccagag cgcgaagtca ttggtgagcc ggtcgagaag
     2221 ttcctcgtag ttatgctgca gttgctgcgc cggcatctgg ccttaccctc ccaatgcccc
     2281 caacttctcg tgctgcgcca gcaacgcgtg gatggccgat accggaatgg cgcggttcag
     2341 cagctccccg ttaacagtgc cccgcccctg atgaagggcg acgaggttcc accctagatc
     2401 gaagacgggc gagccggagg agccatagtc ggtgttgctg cggtgataga tgcgcgtccg
     2461 gctggtgttg accgcggcga agctgtcggt gtcgatgctc agctgcagcg gctgcccatc
     2521 tgggtgatgc aggacgagca aggcgtgcgc ggcagcgaag tcatgcggtg tggcccgtaa
     2581 ttgcagccaa ccacggggtt ggccgcgccc atccggggtt gctttagcgc ccacgaccgc
     2641 gtcacccggc cgcccggcca cgcgcagcag ggcgtagtcg agatgatccg gcggcacatc
     2701 gggcggcgtg cgcgggttta tgggtgtctt gggatgcata agcgggtcat cgctggggta
     2761 tggcgggcta tggtcgatga gccagtcagg caagaccggg ccaagtggcg cgcccgcacc
     2821 gccaagcgcg ccgtcaggca ggaacatgaa gtcgaagcgg aaccctagcc cggccgggtc
     2881 ggcgccctcg acgacattgc gcacaacgtg gtaattagtt agcaccgcct ccggcccgac
     2941 gaggaagcct gtcccacgcg gctggccgcc gtaactgatc aggcagaccc ggttttcggc
     3001 cgccgctgct cgctcgatcc acgttcgggc gtccagtgtg cccagatgct tacgaatgat
     3061 ggcctcaatg cccggcaggt cggttgcttc cgccgggaac tccatcgcca gaccatggcc
     3121 ctgcatgaac aggtagaggt ttggatcggt cggattgaca gtatgagcca ctactaccag
     3181 ctgatccaca attgtgcgcc ggttaaaatg gacaaccacc tcgcggtaga tctccggctc
     3241 gttacgaccg atgatgatcc tgctaactga ggtattgagc cgaaggagaa cgtcctccaa
     3301 ctcctgcagc gacagcgccc gcatgaggat gtctctaagc tcgatgatct ctgggccttc
     3361 tagacgcagc atagagcctc ctggttggtc aatggcaacg acgactggca ccgattatag
     3421 cagaaggccg ctgcctgcca gcaatccggc gcaaacggag cggccccttt cgccaaccgg
     3481 cttccagccg gtttcgtctg ccagtggcgg gtttgcaacc cgccgtgccc cgccccgcaa
     3541 atagatcgac gccagaccgt agagacgggt cgccgggcgt agagacgggt cgccgacccg
     3601 tctctacacc tggcgtccgg gcgtgaaccg cttctgtctc agttgcgcaa gatcgccggc
     3661 agcgagacca cgccaaaggg caagtactcc ttgatgttca ccacgtcgct gctccagcag
     3721 ccggggtcat acatgacgct gtccgagtcg ctgccgttgg ccgtgcgcgc ccagacgctg
     3781 accgtgtgcg tgcccgcggc cagcccctcg tagaagccgc tcatggtggc gttgcggccg
     3841 acgccgctgc caatctcgct ggccttcagc acggcgcgga tgcggcccag cgaggtcgcc
     3901 gcgtcgtcca cccgcagctc gaattgcagg cccgtgccgt cggtgctgcc ggcgtagagg
     3961 cggccgttga attgcagctc caccagcgac tcagccgcct ggacatcgaa cgtgcccacg
     4021 tcggcgatct tggcatacgt gccgctgacg tcgtcgatcc ccgtgcagga cagttccagc
     4081 accgtggccc gcaccgtcgg ccgggccgcc ggttgggccg ccagacggcc ggcggcgaag
     4141 ccagcggtga tcagaaagag ggcgacggcc gcgaacaaca gcaggccgcc gcgaagggaa
     4201 gaaccacttc gagaagacat ggattttctc catagttggg tctggccggg gctgccggaa
     4261 tgggacgcgg gcga
//
LOCUS       DAOSMQ010000268         4253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20834_length_4253_cov_3.732968, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000268 DAOSMQ010000000
VERSION     DAOSMQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20834_length_4253_cov_3.732968"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PK829_09550"
     CDS             complement(<1..1339)
                     /locus_tag="PK829_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOU41500.1"
                     /translation="MVNRSRLRKFMLEFFNDDELADLIFDYFPDARPLMGAGMTLGEK
                     TRALIDFAARHDRLDHLVIVVEKMRTDAYCQFFATDPDLPPEPVTSARDPNRIFISYA
                     NIDAEFASQLAADLRAQGYDIWIAPDSILPGEKWVAAIERGLRESGIFLLVLTPESVD
                     SKWVSQETQVAIMLENEGQMRIFPLRVRRADVPLLLSTRQHISFEMDYDRGLRALLAV
                     LRPSSAKSMPAPLPAPRQPMAETVVVESDEYPVLIIDWPDQPRQEVPLSRMVTTVGRA
                     ADNDLVLALPIVSSHHLRLETVDEGSGYRVNIIDLGSRNGTFVGGRRLSPNAPQPLEP
                     GDVVHLGDRVGRSISLILQPGVTLAEDAPLPPLARPAAPVPRPTPAAPPAVAAPSANQ
                     DRPPQAKPVVTGLPPMNGARMPPAAAATPAAAATPAAATTPAAATTPAAATTPA"
     gene            1501..2169
                     /locus_tag="PK829_09555"
     CDS             1501..2169
                     /locus_tag="PK829_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOU41501.1"
                     /translation="MNVGILGSGAVGQALGSKMAELGHDVVLGTRDPANVDETKGYAP
                     SLGEWLASVDGAARLGTFAEAAAHGELIVNALNGLAAVETLLPLAASLAGKVIIDITN
                     PLDFSRGMPPSVLTYDGASLAEAIQQALPDARVVKSLNTLTAALMVNPRQLAGGDHTV
                     FVAGDDAAAKAEVSELLRTFGWSDILDMGGLAAARGLELWLPLWLQLFGVLGRPSFNL
                     KIVR"
     gene            complement(2313..3740)
                     /locus_tag="PK829_09560"
     CDS             complement(2313..3740)
                     /locus_tag="PK829_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41502.1"
                     /translation="MNAMKRRIVVLLVLLALALAACGGGQEQEPAAEAPAAQPTEAIA
                     EMEPTAMATEMVAETMDEPTAEATAMMDEPTAEATEMMMDEPTAEATEMAMTDADMAV
                     DPLMLDGSAAGLRVALNRLLGEHLLLAASATNAALDGRTADYDAAAAALDENSVDLTS
                     AITAVYGEDAGASFLEQWREHIGFFVDYTVATAAGDSAGQQAAADALAAYTTSFAEFL
                     AGATELPAEALEANLQMHVAGLTAVIDAQAAGDETAYYTALHEAYVHMASTAEALSGG
                     IAAQQPDMFAGDGMSSAAGLRVALNNLLGEHLFLAGAATDEALNGNTPGFEAAAAALD
                     ENSVALADAITSVYGQEAGDEFLAQWRAHIGFLVDYTTATAAGDEAGQTAAGEALSGY
                     ITDFAAFLAGATGLPAEALEAGLQEHVAGLTAIVNAQAAEDAPTAYSEVRMAYLHMQA
                     LGDALSGAIAGQFPEMFPMEGMAEG"
BASE COUNT          661 a         1377 c         1480 g          735 t
ORIGIN      
        1 cagccggcgt ggtcgccgca gccggcgtgg tcgccgcagc cggcgtggtc gccgcggccg
       61 gcgtggccgc cgcagccggc gtggccgccg cagccggtgg catccgcgcc ccgttcatgg
      121 gtggcaagcc ggtgaccacc ggcttggcct gcggcggacg gtcctggttg gcggaaggcg
      181 ccgcaaccgc cgggggcgcg gccggagtcg gccgcggcac gggagccgcc ggccgggcca
      241 gcggtggcaa gggcgcgtcc tcggccaggg tgacgccggg ctgcaagatg aggctgatcg
      301 accggcccac ccggtcgccc aggtgtacca cgtcgcccgg ctccaacggt tggggcgcgt
      361 tgggggacag gcgacggccg ccgacgaacg tgccattgcg gctgcccagg tcgatgatgt
      421 tgacgcgata gccgctgccc tcatcgaccg tctccaggcg cagatggtgg ctggagacga
      481 tgggcagcgc cagaacgagg tcgttgtcgg ccgcccggcc gacagtggtg accatgcggc
      541 tgaggggcac ttcctgacga ggctggtcgg gccagtcgat gatgagcacg ggatattcgt
      601 ctgactcgac gacaaccgtc tcggccattg gctggcgcgg ggccggcagc ggcgcgggca
      661 tggacttggc gctgctgggc cgcaagacgg ccaacaatgc ccgcaatccg cggtcataat
      721 ccatctcgaa ggagatatgc tggcgcgtcg agagcaggag cggcacatca gcccgccgca
      781 cgcgcaatgg gaaaatgcgc atttggccct cgttctccaa catgatggcc acctgcgttt
      841 cctgcgacac ccatttggag tcaacgcttt cgggggtcag cacgagcagg aagatgccgc
      901 tctcgcgcag gccgcgctcg atggcggcga cccacttctc accgggcagg atgctatcgg
      961 gggcgatcca gatgtcgtag ccctgcgccc gtaggtcggc cgccaactgg ctggcgaatt
     1021 cagcgtcgat attggcgtag ctgatgaaaa tgcggttggg gtcgcgcgcg ctggtcaccg
     1081 gttcgggcgg caggtcgggg tcggtggcaa agaactggca gtaagcatcc gtccgcatct
     1141 tctcgacgac gatgacgagg tggtcgaggc ggtcatgcct ggcggcgaag tcgattagcg
     1201 cgcgtgtctt ttcgccgaga gtcatccccg cgcccatgag cggccgcgcg tcggggaaat
     1261 aatcgaaaat caagtccgcc aactcgtcgt cgttgaagaa ctccagcatg aacttccgca
     1321 agcgcgaccg attgaccatg ttaaaccagc ctctctcagg taaaaagatt atatgcagag
     1381 agaaagggat taacaagatg atctacctgg tcgaaagcag cagtcccctt gacaccgata
     1441 ccgacctgta tatactcatg attgcaatac agacctgtct catgtggtga ggtgatgact
     1501 atgaacgtag gtatacttgg atcaggagcc gtgggccaag ccctggggag caagatggcc
     1561 gaattgggcc acgacgtagt gctgggcacg cgcgacccag ccaatgtgga cgaaacgaag
     1621 ggctacgccc ccagcctggg cgagtggctg gccagcgtgg acggcgcggc ccgcctgggc
     1681 acgtttgccg aggcggccgc ccacggcgaa ctgatcgtca acgcgctcaa tgggctggcc
     1741 gcggtggaga cgttgctgcc gttggccgcg tcgctggccg gcaaggtgat aatcgacatc
     1801 accaacccgc tcgacttctc gcgcggcatg ccgccgtcgg tgcttaccta tgacggcgcg
     1861 tcgctggccg aggccattca gcaggctctg ccagacgcgc gcgtggttaa gtcgctcaac
     1921 acgttgacgg ccgcgctgat ggtcaacccg cgccagctgg ccggcggcga tcacaccgtc
     1981 tttgtcgccg gcgatgacgc cgcggccaag gcggaggtca gcgaactgct acgcactttt
     2041 ggctggagtg acatcctcga catgggtggc ctggccgcgg cccgcgggct ggagctatgg
     2101 ttgccgttgt ggttgcagct gtttggcgtg ctggggcggc cgtcgttcaa cttgaagatc
     2161 gtccgctaag cgggccgcac ttccccatga acggcgcagc gccgttggct tgtgaccaac
     2221 ggcgctgcgc cgttcttcca atacttagcc ggggcaatca ggccgtctat tggacaggcc
     2281 catatccccc gacgctaggg ctggtggctt tcctagccct ctgccatgcc ctccatcggg
     2341 aacatctccg ggaattggcc agcgatcgcg cccgacagcg cgtcgcccag cgcctgcatg
     2401 tggaggtagg ccatgcgtac ctcgctgtag gccgtcggcg cgtcctcggc cgcctgggca
     2461 ttcacgatcg ccgtcagccc ggccacgtgc tcttgcagcc ccgcttccag ggcttcggcc
     2521 ggcagtccgg tcgcccccgc caggaaggcg gcgaagtcgg tgatgtagcc gctcagggcc
     2581 tcgcccgccg ccgtctggcc ggcttcgtcg cccgcggccg tggccgtggt gtagtcgacc
     2641 aggaagccga tgtgggcgcg ccactgcgcc aggaactcgt cgccggcctc ctggccatag
     2701 actgaggtga tggcgtcggc cagagcgacc gagttctcgt ccagcgccgc cgcggcggcc
     2761 tcgaagcccg gcgtgttgcc gttgagggcc tcatcggtgg ccgcgccggc caggaagaga
     2821 tgctcaccca gcaggttgtt gagggccacg cgcagcccgg cggccgagga catgccatcg
     2881 ccggcgaaca tatccggctg ttgcgcggca atgccgccgc tgagggcctc agccgtgctg
     2941 gccatgtgga cgtaagcttc gtgcagggcg gtgtagtagg cggtctcatc gccggccgcc
     3001 tgcgcgtcga tgaccgccgt caggccggcg acgtgcattt gcaaattggc ctccagcgcc
     3061 tcggccggta gctccgtcgc cccggccaga aactccgcga acgaagtcgt gtaggccgcc
     3121 agcgcgtctg cggctgcctg ctgcccggcg ctatcgccgg cggcggtggc cacagtgtaa
     3181 tccacaaaga agccgatgtg ctcgcgccac tgctccagga aggaagcgcc ggcgtcctcg
     3241 ccataaacgg cggtgatggc cgacgtcagg tcaaccgagt tctcatccag cgcggcggcg
     3301 gcggcgtcat agtcggctgt gcggccgtcg agagcggcat tagtggcgct ggccgccagc
     3361 aggaggtgct cgcccagcag ccggtttaga gccacgcgca gcccggcggc cgagccgtcg
     3421 agcatcagcg gatcgacggc catatcggcg tcagtcatgg ccatctcggt cgcttcggcc
     3481 gtcggctcgt ccatcatcat ctcggtcgct tcggccgtcg gctcgtccat catcgcggtc
     3541 gcctcagccg tcggctcgtc catcgtctcg gcgaccattt cggtggccat cgccgtcggc
     3601 tccatctccg ctatcgcttc ggtgggctga gccgcgggcg cttcggcggc cggttcctgc
     3661 tcttgcccgc cgccacaagc agccaatgcc agggccaaca ggacaagcaa gacaacaatc
     3721 cgtctcttca ttgcgttcat ctctactcct attgtagttg cgagcggccg gcgtgttgtg
     3781 gccacacggc ggcggctcca ttttcttgac gggcaacaca ggcgcggtga acggagccgg
     3841 cgggaaggct gccggcgctc cggggtcatg ctgtgatgac tgcggtctgt gttgctcctc
     3901 gattgaaccg ctttggcgcg gtctatcgtg gaatacgctt gcctcggggg tctacatcgg
     3961 cgggcagagg gccgctcatt tcacgctcat agttgcgcct gggccggcca cgccgtcagg
     4021 tgcgcctcgc caggggaaga gggcagattg gttgtggtat cgtcgggcgg gtctgtagcg
     4081 gtgactgggg ggcagcggtg ggatgaatgc ggagcctggg cgctggcacc gccgcttttg
     4141 cctctaaggg ggcggggcgg gccgcgcggc ccgccccggc tcactttacg gcgtggctgc
     4201 cgcggctggc gttgcctgag ccgcccgggc cgccaaagac cagcgcgccg ctc
//
LOCUS       DAOSMQ010000269         4234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20980_length_4234_cov_4.289064, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000269 DAOSMQ010000000
VERSION     DAOSMQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20980_length_4234_cov_4.289064"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..338
                     /locus_tag="PK829_09565"
     CDS             21..338
                     /locus_tag="PK829_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOU41503.1"
                     /translation="MSGTISVIPTLALARIVEVTITDDTLAVDLEDGRSIAIPIGWYP
                     RLSQATPAEREHFEISGAGYGIHWPDLDEDVGVEGMLLGKKSGESASSLGRWLAQRRE
                     AAP"
     gene            357..1886
                     /locus_tag="PK829_09570"
     CDS             357..1886
                     /locus_tag="PK829_09570"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU41504.1"
                     /translation="MSQPDNSQLLRFITDNFGLEDLKTLCFNLGVEFDDLGGEGREAK
                     ARELVKWLQNRSRLDHLIAHLSLARPEQYRQHFHETPFIPPTPARLRRDPRQVFISHA
                     HQDGALAQKLARDLQARGYPIWIAPDNIRIGEDWPDAIDRGLDESGVVVVALTPAAMT
                     SYWVRKETSAARLLEGRGLARLVLLDVAECDLPSSWLTYQFANFRDDYAAGLNQLLQA
                     LDGAPTPAAPVETGRRPVSSLPADSSAETGRRPVSTTSPDRRIHAQTSIELIRIPAGP
                     FLYGSTDDNELAFKDQKPQRVVDLPEFWIGRTPITNAQFARFVAATDYRTSTQLRGGQ
                     WAQLGNEKGSEILGIDWQHPQGPGSSLAGKDDHPVTFISWYDAQAFCDWARLALPQQE
                     QWEKAARGTDGRLWPWGNEPPTEERCNFDSFDTTPVGLFSPQGDSPYGCVDMIGNVWE
                     WTASVLWRWPPIRYGLRGGAYYNDMAFVLNPAYRWDSEPEDGGQGYGFRVIERIADGD
                     F"
     gene            complement(1960..2352)
                     /locus_tag="PK829_09575"
     CDS             complement(1960..2352)
                     /locus_tag="PK829_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOU41505.1"
                     /translation="MTLTIEGYLNAAIDSLDAAELLLSSGYPGIAASRAYYAMFYVAE
                     AFLLEQGQEYGKHAGVIGAFGRDVAKVGIVPQIFHRYLIDGQTARSLADYRGDPLTAD
                     EAQLQTDRAREMLAFARQHFAGRSSSSA"
     gene            complement(2349..2669)
                     /locus_tag="PK829_09580"
     CDS             complement(2349..2669)
                     /locus_tag="PK829_09580"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOU41506.1"
                     /translation="MTGELALVLQQLRAGLEAQYGDRLDRVLLYGSRARGDAGPESDV
                     DVLVVLRGEVDPLTEIHRNSQLVADLSLEHDLVLSCVYMSNSELAEGDDLFLRNVKRE
                     AIPV"
     gene            complement(2715..>4234)
                     /locus_tag="PK829_09585"
     CDS             complement(2715..>4234)
                     /locus_tag="PK829_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41507.1"
                     /translation="AFTGERPSSRRFFAGGVALTLLTQTYVQAWGVLPQVALLLLLYR
                     RRVPRRAFLASAAVFAAAALLYALGLAGRAGINAGKAGNFLAGGRLGLSDIGLRHAAR
                     LVNGIDFRPAYAAGSPPGALWPALSLAAVVVLSVALLAGLARAVLALRHDGPERRVAI
                     VLLVWFGLPVLLTSVAGAFDVHPHYLLLTLPAGHALAAWGAARRQDLTGGRPPIRSQP
                     TTTRHPPVRSAVAIAALVGIGAIFAHDLYRANALVAQQPTQPHFDGWSLAAAAEAGQS
                     LRALVLADDGPFPRRIAADGDKALLSGLSATAVQPVRGVAYPDFVLLPAEGAMVYVVE
                     GDGGRPAWLRPLLQDEPARAVSFADGLRLAFARTRLGAAAAAMQAATPVNWPSDAGLT
                     LAGYTLDETAGGLELVTVWRVDALSPDRGAWYVAGSYHLSDTAGHLLANADAHGQWAY
                     RWEMGDVYVEHVTLPGPIPAGARLEIGLFDSVRGVAYTLFDNGAAVGHYTIPPQP"
BASE COUNT          744 a         1361 c         1427 g          702 t
ORIGIN      
        1 gaacatagag aggctgcggc atgagtggga caatttctgt tattccaacg ctggctctgg
       61 cacggatcgt tgaggtaacg attaccgacg atacgctggc cgttgatctg gaagacggcc
      121 gctccatcgc catccctatc ggctggtatc ctcgtctatc ccaagccacc ccggctgaac
      181 gcgagcactt cgagatcagt ggcgcgggct atggcatcca ctggcccgat ttggacgagg
      241 atgtaggtgt ggaggggatg ttgctcggca agaagtcggg cgaaagcgcg tcgtcgttgg
      301 gacggtggtt ggcgcaaaga cgagaggccg cgccgtaaac gcggttgcct aatcacatga
      361 gccagcccga taactctcaa ctcctccggt tcatcacaga caacttcggt ctggaagacc
      421 tgaagacgct ctgcttcaac ctgggtgttg agttcgacga tcttggcggg gagggacgtg
      481 aggctaaggc gcgcgagctt gtcaagtggc tgcagaaccg ttcgcggctg gatcacctca
      541 tcgcccatct ttctctcgcc cgcccggagc agtaccgcca gcacttccac gaaacgccgt
      601 tcattccccc aacgcccgcc cgtctgcgcc gcgacccacg gcaggtgttt atcagccacg
      661 cgcatcagga cggcgcgttg gcgcaaaagt tggccaggga tttgcaggcc cgcggttatc
      721 cgatctggat cgcccccgac aatattcgca tcggcgagga ttggcccgac gcgatcgacc
      781 gcgggctaga cgagagtggg gttgttgtgg tcgccctgac ccccgcagcg atgacttcct
      841 actgggtgcg caaggaaacg agcgccgccc gccttcttga gggccggggg cttgcccggc
      901 tggtgctact ggatgtggcc gagtgcgatc tgccgtcttc gtggctgacc tatcagttcg
      961 ccaatttccg ggacgattat gccgccggcc tgaatcagtt attgcaggct ctggatggcg
     1021 cgcccacccc cgccgcaccc gtagagacgg gtcgccgacc cgtctcatcc ttgccggctg
     1081 attcgtccgc ggagacgggt cgccgacccg tctctacaac ctcgcccgat cgccgcatcc
     1141 acgcccagac cagcatcgaa ctcattcgca tccctgccgg gccgtttctc tatggctcga
     1201 ctgatgacaa cgagttggcg tttaaggacc agaagccgca gcgagtcgta gacctgcccg
     1261 agttctggat cggtcgcacg cccattacca atgctcagtt tgcccgcttt gtggcggcga
     1321 cggactatcg aacgagcacc cagcttcgcg gtgggcagtg ggcgcaattg ggcaatgaaa
     1381 aggggagcga gatcctgggc attgattggc aacatccgca agggcctggg tcttcgctgg
     1441 cgggcaagga tgatcatcct gttaccttca ttagttggta cgacgcgcag gcgttctgcg
     1501 actgggcgag gttggcgttg ccacaacagg aacaatggga aaaggcggcg cgagggacgg
     1561 acggccgtct ctggccctgg ggcaacgagc cacccacaga ggaacgctgc aacttcgaca
     1621 gctttgacac aacgccagtg ggcttattct cgccgcaggg cgatagccct tatggctgtg
     1681 tagacatgat cggaaacgtg tgggagtgga cggcttcggt tctgtggcga tggccaccca
     1741 ttcggtacgg gttgcgtggc ggagcctact acaacgatat ggccttcgtc ctcaatccag
     1801 cctatcgatg ggatagcgag ccggaggacg gcgggcaggg gtatggcttc cgggtgatcg
     1861 agcggatcgc cgatggtgac ttctgagttc tggcttctga atcttctgct ttcggtgggg
     1921 tctgggggcg gccgccccca gcggccggcg ggaagggagc taggctgagg aagaggagcg
     1981 gccggcgaaa tgctgccggg cgaaggcgag catttcgcgg gcgcggtcgg tctgcaattg
     2041 ggcttcgtcc gctgtaagcg gatcgcctcg atagtcggcc agcgaccgtg ccgtctggcc
     2101 gtcgatcagg tagcggtgaa agatctgcgg cacgatcccg actttcgcca cgtcgcggcc
     2161 gaaagcgccg attaccccgg catgtttacc gtactcctgc ccttgctcta gcagaaacgc
     2221 ctcagccaca taaaacatgg cgtaataggc gcgtgacgct gcaattcccg gatagccact
     2281 cgataaaagc aattcagcag catccaggct gtcgattgct gcgttcaaat acccttcgat
     2341 cgtaagtgtc ataccggtat ggcctcgcgt ttcacattgc gcaggaagag atcatcacct
     2401 tcagccagct cagagttgct catatagacg caagagagaa ctagatcatg ctccagtgag
     2461 aggtcagcca ccagttggct attgcggtga atctctgtga gcggatcgac ctccccccgc
     2521 aacaccacca acacatccac atccgactcc ggccccgcgt cgccgcgggc gcgcgagccg
     2581 tagagcagca cccggtcgag ccggtcgcca tattgcgcct ccaagccagc acgcagttgt
     2641 tgcaggacga gagctagttc gccggtcatt gtcatcatta tagcacggcc gtagcgcagc
     2701 ctgtcaggct gccgctacgg ttgtggcggg attgtgtaat ggcctacggc cgcgccattg
     2761 tcgaacaacg tatacgccac gccgcgcacg ctgtcgaaca ggccgatctc cagccgcgcc
     2821 ccggcgggaa tggggccggg gagggtgacg tgctcgacgt agacgtcgcc catctcccag
     2881 cggtaggccc actggccgtg ggcgtcggca ttggccagca ggtggccggc ggtgtcgctc
     2941 aggtggtagc tgccggcgac gtaccacgcg ccgcggtcgg ggcttagcgc atccacgcgc
     3001 cagacagtga ccagttccag gccgccggcc gtctcgtcca gcgtgtagcc ggccagtgtc
     3061 agcccggcgt cgctgggcca gttgaccggc gtggccgctt gcatggccgc ggcggctgcg
     3121 cccaatcgcg tgcgggcgaa agccagccgc agcccatccg cgaatgacac cgcccgcgcc
     3181 ggttcgtctt gcagcagcgg ccgcagccac gccggccggc cgccgtcgcc ctcgacgacg
     3241 tagaccatcg ccccctcggc cggcagcagg acgaagtcgg gatacgccac gccgcgcacc
     3301 ggctgcacgg cggtcgcgct caggccgctg agcagggcct tgtcgccgtc ggcggcgatg
     3361 cgccggggga aggggccgtc gtcggccagc accagggcgc gcagcgattg gcccgcctcg
     3421 gccgccgcgg ccaacgacca gccgtcgaag tggggttgcg tcggctgctg ggcgacaagc
     3481 gcgttggccc ggtagaggtc atgggcgaag atggcgccga tcccgaccag cgcggcgata
     3541 gctacggccg acctgaccgg tgggtggcgg gtagttgtcg gctgggaacg gattggtggc
     3601 cgcccacctg tcaggtcttg ccgccgggcc gcgccccacg ccgccagcgc gtgcccggcc
     3661 ggcagcgtca gcagcaggta gtgggggtgg acgtcgaacg cgccggcgac gctggtcagc
     3721 agcaccggca ggccgaacca gaccagcagg acgatggcga cgcgccgctc cggcccgtcg
     3781 tggcgcaggg ccaggacggc gcgggccagc ccggccagca gggccacgga caggacgact
     3841 acggcggcca gcgacagcgc cggccagagc gcccccggcg ggctgccggc ggcataggct
     3901 gggcggaagt cgatgccgtt gaccagccgg gcggcgtggc gcaggccgat gtcgctcagg
     3961 cccagccggc cgccggccaa gaagttgccc gccttgccag cgttgatgcc ggcgcggccg
     4021 gccaggccga gggcatagag cagcgccgcg gcggcgaaga cggccgcgga cgccaggaac
     4081 gcccgccgcg gcacgcgccg ccggtagagc agcagcaaca gggccacctg tggcagcacg
     4141 ccccaggcct gcacatacgt ctgcgtcagc agcgtcaggg ccacgccgcc ggcgaagaag
     4201 cgccgcgacg acggccgctc gccggtgaac gccg
//
LOCUS       DAOSMQ010000270         4233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_20986_length_4233_cov_3.879368, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000270 DAOSMQ010000000
VERSION     DAOSMQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20986_length_4233_cov_3.879368"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PK829_09590"
     CDS             complement(<1..925)
                     /locus_tag="PK829_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU41508.1"
                     /translation="MSELNSFPFDWHEVARLALASRLMDELEERELTPGGLVTYQFSA
                     RGHELGQLLLAQLLDRPFDAASVYYRSRPFMLASGLTLTEALAADMARAGGMSDGRDV
                     GVVFNMPRRGRALVLPMAADVGSQFTPAAGWAQALRYRRDHLGEADVADSIAVAFGGE
                     GSVASNGFWSALTMATTLRLPLLVVVEDNNYAISVKSGLQTPGGNIAANLASFHDLAI
                     WDGSGVEPAATAALVAAAVAHVRGGAGPGLLRLTVPRLSGHSSVDNQAYKTEDERDDE
                     WARDPISALRAALVPALMDARAWQLLADDATA"
     gene            1176..3488
                     /locus_tag="PK829_09595"
     CDS             1176..3488
                     /locus_tag="PK829_09595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOU41509.1"
                     /translation="MLATLVCLGAWAALLAPTASGQGDPVEPNAPAIVGGREAEPGEW
                     PWQVALIHVGGDPYDDQYCGGTLISPVWVVTAAHCAAGETAGRIQVLAGIHNLRTPDR
                     GFLRLDVERIILHPNYGQATQYDSDIALLELSAPATFRPMTTTQLPIDGVTLVAADID
                     SLTGVEATVTGWGNTSSSEIDFPDTLQEVEVPIVSNADCSQAYPNGITSNMVCAGVPE
                     GGVDSCQGDSGGPLVIFNNATSRWELAGIVSWGYGCALPGQPGVYTRVSSFSEWITQE
                     TGVGYPNFRLVVAPAQADVCAGTTAQMTVSIVSLAGFDQPVELSLAGLPPGGHASFQP
                     PVVAPSMTSSLTIATANIAAGRYELLVRGMSGALSHGATLTLLVTAAAPQPPHPLSPT
                     ANVVGHTVAPRFSWTAVSAAGQYHLQVADEPSFQHIIRDQLVDGTTLALDDWLEPNTA
                     YYWRVAASNTCGASVYSPIYRLTTGQAYCREPGLQLPDGDPAGVMDTMTLSTAGILAD
                     VDVSLTITHEWPADLTADLTYMPAARVVTLFNRPAQECPGVDDMISLFDDEATIPVSS
                     ACSPVPPAIGGRVLPETPLAALDNQPLAGAWRLRVADQFTVGLGQLESWCLLPSLAAD
                     YCDQVTDVPAAECAALDALFTGTDGWSWAGRAGWFTRTKACLWQGVSCTGGHVTALRL
                     PENGLNGPLPAAIGALTQLQALDLSGNAALTGLLPSTLIDLSLNQFHFDGTALCVPPG
                     RDFTNWLNGIDDLRVSGNACAQSFLPLLRR"
     gene            complement(3462..>4233)
                     /locus_tag="PK829_09600"
     CDS             complement(3462..>4233)
                     /locus_tag="PK829_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU41510.1"
                     /translation="IGIDIIGGSSMGAFIGGCLARGMVAADGLRGSAGRANARSLLDR
                     TFPYTAIMTSRKITDVMRDYFGDRQIEDLWQPFFCVSTNLSIAAPVVHERGPLWRAVR
                     ASTALPGVFSPILNEKNEMLVDGGVMNNFPVDVMAARYEVGQIIASNVSPHREKPYPH
                     PFGESISGWQALWSRVNPFGRPMRVPTLGSVILRTVEVNSLYQRKAAEQLADVLIEPD
                     VADFNLLDFAAIRALEQRGYEAARAALALTSTPQQRQK"
BASE COUNT          699 a         1426 c         1372 g          736 t
ORIGIN      
        1 gggcggtggc gtcgtccgcc agcagttgcc acgcccgcgc atccatgagc gccggcacga
       61 gggcggcgcg cagcgcggag atggggtcgc gcgcccactc gtcgtcgcgc tcgtcctccg
      121 tcttataggc ctggttatcg acgctggagt ggccggacag gcgcggcaca gtcaggcgca
      181 gcagccctgg ccccgcgccg ccacgcacat gggccacggc cgcggccacc agcgccgccg
      241 tcgcggccgg ttccacgccg ctgccatccc aaatggccag gtcgtggaac gaggccaggt
      301 tggcggcgat gttgccgccc ggcgtttgca gcccgctctt gaccgaaatg gcgtagttgt
      361 tgtcctcaac cacgacaagc agcggcaggc gcagcgtcgt ggccatcgtc agggctgacc
      421 agaagccatt gctggccaca gagccttcgc cgccgaaggc gacggcgatg ctgtcggcta
      481 cgtccgcctc gcccaggtgg tcgcgccggt agcgcagggc ctgcgcccag ccggccgcgg
      541 gcgtgaactg cgagccgaca tcggcggcca tcggcagcac cagcgcccgg ccgcggcgag
      601 gcatgttgaa aaccacgccg acatcgcggc cgtcgctcat gccgccggcg cgagccatgt
      661 cggcggccag cgcctccgtc agcgtcagcc cgctggcgag catgaacggc cgggagcgat
      721 agtagaccga ggcggcatcg aaggggcgat ccagcaattg ggccaggagt aattgtccca
      781 actcatggcc gcgggcggaa aactggtacg tcaccaggcc gcctggcgtc agttcgcgct
      841 cctccagctc gtccatcagc cgcgaggcca gcgccagccg ggcaacttca tgccagtcga
      901 aaggaaaaga attcagttcg ctcacgccgt gctcctgtgg tcgtccggtg gctgtcgccg
      961 cgggtcaatg ttgtaacccg gggcagccga tttgccaaga cgggtaatgt tgctttttgc
     1021 cgttgccgag ctattctccc atctgtggac ggtgggtgtt gtgctagtcc agccgttccc
     1081 cgccattgtg ttcgcgtggc agctttgcag gggttcctat gcgtaaccgc tcaatcgcac
     1141 cgtcgttcgt cagcagtttg cgtctgcgtt tgtgtttgct cgccacactg gtctgcctgg
     1201 gggcgtgggc ggccctgttg gccccaacgg ccagtggcca gggcgacccc gtagaaccca
     1261 acgcgccggc cattgtcggc ggacgcgaag ccgagccggg cgagtggccc tggcaggtcg
     1321 ccctcatcca tgtcggcggc gatccttacg acgaccagta ctgcggcggc acgctgatta
     1381 gccctgtttg ggtcgtcacg gccgcccact gcgccgcggg cgagacagcc ggccgaatcc
     1441 aggtgctggc cggcatccac aacctgagaa cgcccgaccg tggcttcttg cggctggacg
     1501 tggagcgcat cattctgcac ccaaactacg gccaggcgac ccagtacgac agcgacatcg
     1561 ccctgctgga actcagcgcg ccggcgacct tccggccgat gaccaccacc cagctgccca
     1621 tcgacggcgt caccctggtc gcggctgata ttgactccct gaccggtgtc gaggccaccg
     1681 tcaccggctg gggcaacacg tcctcgtctg agatcgattt tccagacacg ctgcaagaag
     1741 tcgaagtgcc catcgtgtcg aacgctgatt gcagccaggc ctatcccaat ggcatcacca
     1801 gcaatatggt ctgtgcgggc gtgcccgaag gcggcgtcga ctcgtgccag ggcgacagcg
     1861 gcggcccact tgtcatcttc aacaacgcaa cctctcgctg ggagctggcc ggcatcgtca
     1921 gttggggcta cggctgcgcg ctgccgggcc agcccggcgt ctacacgcgc gtgtcgtcct
     1981 tctcagaatg gattactcag gagaccggcg tcggttaccc caacttccgc ctggtcgttg
     2041 ccccggccca ggccgatgtc tgcgctggca cgaccgcaca gatgaccgtg agcattgtca
     2101 gcctggccgg gttcgaccaa ccggtggagt tgtcgttggc cggactgccg cccggcggcc
     2161 acgcctcgtt tcaaccgccc gtcgtcgcgc catcgatgac aagcagcctg accatcgcca
     2221 cggccaatat cgctgccggc cgttacgagt tactcgtgcg cggcatgtcg ggcgcgctca
     2281 gccacggggc gacattgacc ctgcttgtca ccgccgctgc gccccagccg ccgcacccat
     2341 tgtccccaac ggccaacgtt gtcggccaca cggtcgcgcc gcgcttctcc tggacggccg
     2401 tgagcgcggc cgggcagtat cacttacagg tggctgacga gccatcgttc cagcatatca
     2461 ttcgcgatca gttggttgac gggacgacgt tggctctaga cgattggctg gagccgaaca
     2521 cggcctatta ctggcgcgtc gccgccagca acacctgtgg cgcaagcgtc tattcaccga
     2581 tctaccggct gacgacagga caagcctatt gccgcgaacc tggtttgcaa ctgcccgatg
     2641 gtgacccggc cggcgtgatg gacacgatga ctctatcgac cgcgggcatt ctggccgacg
     2701 tcgacgtcag tctgacgatc acgcacgagt ggccggcaga cctgacagcg gacttgacct
     2761 atatgccggc cgccagagtc gtcacgctgt tcaaccggcc cgcccaggag tgccccggcg
     2821 tggacgacat gatcagcctc ttcgacgacg aggcgaccat accggtcagc agcgcttgca
     2881 gcccggttcc acccgccata ggcggtcgcg ttctccccga gacgccgctg gcagcgctgg
     2941 ataatcaacc gctcgccggc gcgtggcgct tgcgggtagc cgatcaattc actgtgggtt
     3001 tggggcagct ggagtcctgg tgcttgttgc catcgctggc ggctgactac tgcgaccagg
     3061 taaccgatgt gcccgcggct gaatgtgccg cgctcgacgc gctcttcacg ggcacggacg
     3121 gctggagttg ggccggccgg gctggctggt tcaccagaac aaaggcttgc ctgtggcaag
     3181 gcgttagttg caccggcggg catgtgacgg ccttacgcct gccggagaac ggtctgaacg
     3241 ggccactgcc ggccgccatc ggcgcgctga cccagctaca ggctctcgac ctgtctggca
     3301 atgcggcgct gaccgggctg ctgccatcca cgctgataga cctgtcgctc aatcagtttc
     3361 actttgacgg cacggctctg tgcgtgccac ccggccgcga cttcaccaac tggctcaatg
     3421 gcatcgacga cctgcgtgta tcgggcaacg cttgcgcgca gtcatttctg ccgctgctgc
     3481 ggcgttgagg tcagggccag cgcggcgcgc gcggcctcgt agccgcgttg ctccaacgcg
     3541 cgtatcgccg caaaatcgag gaggttgaag tcggccacat ccggctcgat gagcacgtcg
     3601 gccagttgct cggcggcctt gcgctggtag aggctgttga cctcgaccgt gcgcaggatg
     3661 acgctgccta acgtgggcac gcgcatgggc cggccgaacg ggttgacgcg gctccagagc
     3721 gcctgccagc cagagatgct ttcgccaaag ggatgagggt agggcttctc gcgatggggg
     3781 ctgacgttgc tggcgatgat ctggcccacc tcatagcgcg cggccatcac atcgacaggg
     3841 aagttattca tcacgccgcc gtctaccagc atctcattct tctcgttcag aattgggctg
     3901 aagaccccag gcagggccgt gctggcccgc accgctcgcc agagcggccc gcgctcatgc
     3961 accaccggcg cggcaatgga caggttggtg gagacgcaga aaaacggctg ccagaggtct
     4021 tcgatctgcc ggtcgccgaa gtaatcgcgc atcacgtcgg tgatcttgcg cgaggtcatg
     4081 atcgcagtat aagggaaggt acggtcgagc agcgagcggg cattggcccg tccggccgat
     4141 cccctcaggc catcggcggc gaccatccca cgggccaaac agccaccgat aaaagcgccc
     4201 atgctggagc caccgatgat gtcgatgccg atg
//
LOCUS       DAOSMQ010000271         4206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21172_length_4206_cov_3.798362, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000271 DAOSMQ010000000
VERSION     DAOSMQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21172_length_4206_cov_3.798362"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1053
                     /gene="fni"
                     /locus_tag="PK829_09605"
     CDS             31..1053
                     /gene="fni"
                     /locus_tag="PK829_09605"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOU41511.1"
                     /translation="MHERRKADHIRINLEEDVAFKSLTTGLEAYFFMHRALPELDLAA
                     VDTGVEVLGKRQRTPLLISSMTGGTARAQAINRELAAGAQAAGMAMGLGSMRAAIEDP
                     SLAATYQVRDVAPDILLFANLGAVQLNYRYGIDQCRRAVDMIQADALILHFNALQEAV
                     QPEGDGNFAGLLAKVEAICRALPVPVVAKEVGWGFAAEDARRLAEAGVAAIDVAGAGG
                     TSWSQVEMHRAPTARHARVAGAFIDWGIPTAVSLRYCRQAAPDLPIFASGGIKDGIDV
                     AKCIALGASLVGLAGEFLRAADGDGAAGVVELADTITTELRVAMFCAGAGDFAALRQT
                     PLHTRF"
     gene            1082..2113
                     /locus_tag="PK829_09610"
     CDS             1082..2113
                     /locus_tag="PK829_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU41512.1"
                     /translation="MTTDLERLSAGFLPAVEAEMRAVLRADDAADPDPFYGMIHYHMG
                     WADEALRPAGGNSGKRIRPVLCLLACAAAGGDWQQALPAAAAVEIVHNFSLVHDDIED
                     QSPTRRGRRTLWTLQGEAQAINTGDAMFALAHLALGRLADRGVAPAVVVRAWRRFDET
                     CVALTRGQYTDMHFETRAQVTVDEYVAMITGKTAVLLAHSAELGALVAGADAARVAHF
                     ADFGLNLGLAFQAQDDLLGIWGDEQLIGKSVSTDIATRKKTLPVLYGLERSAPLRALY
                     ADEPADAADFVSSVVPLLDAAGARTYTAECVALYTDKALVHLAAAQPQEPAAAALYQL
                     TDMLLRRNY"
     gene            complement(2222..>4206)
                     /locus_tag="PK829_09615"
     CDS             complement(2222..>4206)
                     /locus_tag="PK829_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU41513.1"
                     /translation="RVDEIDRQFRRGLLTEEEQYQRTIEQWNDAKDYIADAVKKALDP
                     NGPLALMSLSGAGKGGFGPITQLAGMRGLMADPQGRIIPVPIQSNFRQGLDALEYFIS
                     THGARKGLADTALRTADAGYLTRRLVDVAQDIIIMEEDCGTNKGIYVRRVDNFGKQTL
                     AERLIGRVSAATVSDPATGEIVIAQDEYFTGELAEAVDALGVEEVYVFSPMTCELRLG
                     ICRKCYGTDLARGKPVELGVAVGIVAAQSIGEPGTQLTLRTFHTGGTASAGGDITQGL
                     PRVEELFEARQRPKGEAVMTEIGGIANIRLIDNVRHAFITDSKLVDDVYEVPAGWSML
                     VENNQEVTAGATLAESGDEIIHARHPGRVTIDENGLRVTWESREERDYEISAGVRLLI
                     GDGQRVSAGDQLTEGSRNPHRILEILGRDAVTQYLLREVQQVYRPQGQNIHDKHFEVI
                     IRKMLSKVSVTDSGDTDMLPGDLIEAPGFQEKNEEVIAEGGQPAQAEPVLLGITKASL
                     NTDSFLSASSFQHTIKVLAGAAIAGKEDRLIGLKENVIIGKLIPAGTGFRVGEEEAEA
                     LPDGILFEGDGGAMRLTSSALIDDDTDDEILAAIAAAAADASRLALVTDYEDAEESES
                     IWDEEEAWDDDDSDEDEEEADETEAVDEEDWEEPGD"
BASE COUNT          668 a         1455 c         1346 g          737 t
ORIGIN      
        1 gacacggccg gccgcgaacc cggcagcgac atccacgagc ggcgtaaggc cgaccatatc
       61 cgcatcaacc ttgaagagga tgtcgccttt aagagcctga ccaccgggtt agaggcgtac
      121 tttttcatgc accgcgccct gccagagctg gatttggccg ccgtggatac cggcgttgag
      181 gtactgggca agcgccagcg cacgccgctg ctcatctcct cgatgaccgg cggcaccgct
      241 cgcgcccagg ccatcaaccg cgaactggcc gccggggcgc aagcggcggg tatggctatg
      301 ggcctcggct ccatgcgcgc cgccatcgaa gacccctcgc tggccgccac ctaccaggtg
      361 cgcgacgtgg cccccgacat tcttcttttc gccaatctcg gcgcggtgca actcaactac
      421 cgctacggca tcgaccagtg ccgccgcgcc gtcgacatga tccaggccga cgccctaatc
      481 ctgcacttca acgctctgca agaagccgtc cagccggagg gcgacggcaa cttcgccggg
      541 ctgctggcca aagtagaggc catctgccgg gcgctgcccg tgccggtcgt ggccaaggaa
      601 gtcggctggg gctttgccgc cgaggacgca cgccggttgg ccgaggccgg cgtggccgcc
      661 attgacgtgg ccggcgcggg cggcacgtcg tggagccagg tggagatgca ccgcgcgccg
      721 acggcccgcc acgcccgcgt ggccggggcc ttcatcgact ggggcattcc cacggccgta
      781 tcgctgcgct actgccgcca ggccgcgccc gacctgccca tcttcgccag cggcggcatc
      841 aaggacggca tcgacgtggc caagtgcatc gccctgggcg cgtcgctggt cggcctggcg
      901 ggcgagtttt tgcgcgcggc cgacggtgac ggcgcggccg gcgtcgttga attggccgac
      961 acgatcacaa cggagctgcg cgtggccatg ttctgcgccg gcgcgggcga cttcgccgcg
     1021 ctgagacaga cgcccctcca cacgcgcttc tagccagcgc gcactctctg gaacacgaac
     1081 catgaccaca gacctggaac gcctgagcgc cggcttcctg ccggctgttg aagccgagat
     1141 gcgcgccgtc ctgcgcgccg acgacgcggc agaccccgac cccttctacg ggatgatcca
     1201 ctaccacatg ggctgggccg acgaagcgct gcgaccggcc ggtggcaaca gcggcaagcg
     1261 catccgcccc gtgttatgcc tgttggcctg cgcggccgcc ggcggcgatt ggcaacaggc
     1321 cttgcctgcc gccgccgcgg tcgagatcgt tcataacttt tctctcgtcc acgacgacat
     1381 cgaggaccaa agccccaccc gccgcggccg gcgaaccctc tggacgctgc aaggcgaagc
     1441 gcaggccatc aacaccggcg atgccatgtt cgccctggcc cacctggcgc tgggccggct
     1501 ggccgaccgc ggcgtggctc ctgcggttgt cgtgcgcgcc tggcggcgct ttgacgaaac
     1561 ttgcgtcgcc ctgacgcgcg gccaatacac cgacatgcac ttcgagacgc gggcgcaggt
     1621 gacggtggat gagtatgtgg ccatgatcac cggcaagacc gctgtattgc tggcccactc
     1681 ggctgagctg ggggcgctgg tggccggggc cgatgccgcg cgcgtggccc acttcgccga
     1741 cttcggcctg aacctgggcc tggccttcca ggcgcaagac gacctgctgg gcatctgggg
     1801 tgatgagcag ctcatcggca aatcggtcag taccgacatc gccacgcgca agaagacgtt
     1861 gcctgtgctc tatgggctgg aacgtagcgc gcctttgcgg gcgctctatg ccgacgaacc
     1921 ggccgacgcg gccgatttcg tcagcagcgt cgtgcccttg ctcgacgccg ccggcgcgcg
     1981 aacgtacacg gccgagtgcg ttgccctcta taccgacaag gctctcgttc acctggcggc
     2041 ggcgcaacca caggagccgg ccgccgcggc gctctaccag ttgaccgaca tgctgctgcg
     2101 ccgcaattac tagcccgcca ggccgcggcc tgcccacagc tgtccacagc aatgaaaaaa
     2161 gaccggtcgg cagataaccg accggtctct cgtttagcgt tcggcaacga ctagccgagg
     2221 gttagtcgcc cggttcttcc cagtcttcct catcgaccgc ttcggtctcg tcggcctctt
     2281 cctcgtcttc gtcactatcg tcgtcgtccc aggcttcctc ttcgtcccag atgctctcgc
     2341 tttcctcagc atcctcgtag tcggtaacca gggccaggcg cgaggcgtcg gccgcggcgg
     2401 cggcgatggc cgccagaatc tcatcgtccg tgtcgtcatc aatcagggcg ctactggtca
     2461 ggcgcatggc cccgccatca ccctcgaaga ggatgccatc cggcaaggcc tcagcctcct
     2521 cttcgccgac gcggaagccc gtgccggccg ggatgagctt gccgataatg acgttctcct
     2581 tcaggccgat gaggcggtct tccttgccag cgatggccgc cccagccagc accttgatgg
     2641 tgtgctggaa cgaactggcc gacagaaaac tgtccgtgtt gagcgacgcc ttggtgatgc
     2701 ccaagagcac cggctccgcc tgcgcgggct gcccgccttc ggcgatgacc tcttcgtttt
     2761 tctcctggaa gcccggcgct tcgatcaggt cgcccggcag catgtcggtg tcgccggaat
     2821 cggtgaccga caccttgctc agcatcttgc ggatgatgac ctcgaagtgc ttatcgtgga
     2881 tgttctggcc ctgcggccgg tagacttgct ggacttcgcg caacagatat tgcgtcaccg
     2941 cgtcgcgccc cagaatctcc aggatgcggt gcgggttgcg cgagccttcc gtcaactgat
     3001 cgccggcgct gacgcgctgg ccatcgccga tgagcaatcg cacgccggca gagatctcat
     3061 agtcgcgctc ttcgcggctt tcccacgtga cgcgcagacc gttctcgtcg atggtgacgc
     3121 ggccgggatg gcgggcatgg atgatctcgt cgccgctctc ggccagcgtc gcgccggcag
     3181 tcacttcttg attgttttcc accagcatcg accagccggc cggcacttcg tagacatcat
     3241 cgaccagctt gctgtcagta ataaaggcat gacggacgtt atcgatcagg cggatattgg
     3301 caatgccgcc gatctcagtc atgaccgctt cgcccttggg ccgttggcgc gcctcgaaca
     3361 actcttcgac acgcggcaga ccctgggtaa tatcgccccc ggccgaagcc gtgccaccgg
     3421 tgtggaacgt gcgcagtgtc agctgcgtgc ccggctcgcc gatggattgg gcggcgacga
     3481 tgccgacggc cacacccagt tcgaccggct tgccgcgggc caggtcagtg ccgtaacact
     3541 tgcggcagat gcccaggcgc aactcgcagg tcatgggcga gaagacgtag acctcttcca
     3601 cgcccaatgc atccacagcc tcagcaagct cgccggtgaa gtactcgtcc tgggcgatga
     3661 cgatctcgcc cgtggccggg tcggagaccg tcgccgccga cacgcgaccg atgaggcgct
     3721 cggccagcgt ctgcttgccg aagttgtcga cgcggcgaac gtagatgccc ttgttcgtgc
     3781 cgcagtcctc ttccatgatg atgatgtcct gggccacgtc caccagacga cgggtcaggt
     3841 agccggcgtc ggccgtgcgc agggcggtat cggccagacc cttgcgcgcg ccgtgggtgg
     3901 agatgaagta ctccagcgcg tcgaggccct ggcggaagtt ggattgaatc ggcacgggga
     3961 tgatgcgtcc ctgggggtca gccatcaggc cgcgcatgcc ggccagctgc gtgatcgggc
     4021 caaagccgcc cttgcccgcg cccgacaggg acatcaacgc gagcggacca ttcgggtcga
     4081 gcgccttctt gaccgcgtcg gcgatgtagt ccttggcatc gttccactgc tcaatggtgc
     4141 gctggtactg ctcctcttcg gtcagcaggc cgcggcggaa ctggcggtcg atctcgtcca
     4201 cgcgct
//
LOCUS       DAOSMQ010000272         4204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21182_length_4204_cov_4.564473, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000272 DAOSMQ010000000
VERSION     DAOSMQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21182_length_4204_cov_4.564473"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2449
                     /gene="uvrA"
                     /locus_tag="PK829_09620"
     CDS             <1..2449
                     /gene="uvrA"
                     /locus_tag="PK829_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU41514.1"
                     /translation="LTGLSGSGKSTLAFDILCLEGARQYLESVGVLIFGLARPPLESI
                     TGLAATISLNQQTSANHSPRSTVGTTTDAYTYVRILFARLGRRPCPHCGGDVPPSFGL
                     PGDEWEDDTGPATDPDAAPAFPCPHCGRPVPELGMAAFSFNKPEGACPTCTGLGSVHT
                     ANLDRLADAGRSLAGGAVAGWVGPQLAYYGDSLLAAARHYGLALTLDTPIRDYSPAAR
                     DLFFFGVESPQFRRHFPDREPPATVRGGRFEGLATAALRRYGEHAGDEDYAARMAGQV
                     VAAVCPDCLGQRLRPESRAVTVAGQTIMAVAGMSLAALAEWLRELLAALSADEHRIAA
                     PLLEDLNARLGRLLEVGVGYLTMARASATLSAGEAQRLRLASLVGSGLTGMLYILDEP
                     TVGLHPRDTERLLRVLRRLRDLGNTVLVVEHDLDVIRAANTVIDMGPGAGRHGGRVVA
                     AGTPAEVAAHPTSLTGAYLAGRAATPPPPRRPPAERALTIAGARANNLRNITARLPLE
                     RLVVVTGVSGAGKSSLVFDILDRAGRARFYGATDTPGEHDAIAGWEAIDKLVTIGQEP
                     IGRRPRSNAATYADVFTPIRELFAAQPEAKQRGFTARRFSFNVPGGRCERCEGAGVLP
                     VTMHLLPPVDVRCPVCHGRRYDAATLAVTYGGHDIAAVLAMTVEEALALFAGVPAVAT
                     RLAVMADVGLGYLPLGQPATTLSGGEAQRVKLARELGRRGGGHTLYLLDEPTTGLHPA
                     DTARLLAVLNRLVDAGHSVVVVEHNLDVIRAADWVIDLGPEGGAAGGELIAAGTPEDV
                     AASPRSLTGAFLRPLLS"
     gene            2941..>4204
                     /locus_tag="PK829_09625"
     CDS             2941..>4204
                     /locus_tag="PK829_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOU41515.1"
                     /translation="MTTDLDLKTTLSARRIVGLWRMITGYRAIYVGAFLCIGLAAVCQ
                     TLFYFLLRYFTDDVLGIEAMRGQLAWVAAGFIGLALAQGLFTFMGGRWATMTSENVIR
                     RLRDYLYDHVQRLTFAYHDKTPTGDLIQRSTSDVETMRRFLGEESVGLGRILSLFVVN
                     FVGLLTLDTRLALISIVVVPVIVVVSIYFFRMIGKRYEIFQEEEAKLSTTLQENLSGV
                     RVVRAFARQDFERQKFEVTNRGRYDSGRKLLVLHAVYWPSTDIIVGIQLIIGYAVGAT
                     MAINGAITVGTFLAYMGMLTWIMWPIRNIGRLIVQMSMALVSFDRVSEIIRVDREPLD
                     VGTHRPDGPPRGEIVFDDVRFAYAEDMAVLNGITFRAEPGQVVALVGPTGSGKTSLVN
                     LLPRFYEYQGSIRLDGVELRDYTRRYLRE"
BASE COUNT          598 a         1493 c         1399 g          714 t
ORIGIN      
        1 cctgactggc ctgtccggct ccggcaaatc gacgctggcc ttcgatatcc tctgtctgga
       61 gggggcgcgg caatacctgg aatccgtcgg cgtgctcata tttggcctgg cccggccgcc
      121 gctggagagc atcaccggcc tggcggcaac catcagcctc aatcagcaga cttcggccaa
      181 ccacagcccg cgctcaaccg tgggcacgac caccgacgcc tacacctacg tccgcatcct
      241 cttcgcccgg ctggggcggc ggccgtgccc ccactgcggc ggcgacgtgc cgccgtcgtt
      301 tggcctgccc ggcgacgagt gggaagacga caccggcccg gcgaccgacc ccgacgccgc
      361 gccggccttc ccctgtcccc attgcggccg gcccgtgccg gagttgggca tggccgcctt
      421 ctccttcaac aagccggagg gcgcttgccc tacctgcacc ggtctgggca gcgtccacac
      481 cgctaacctc gaccggctgg ccgacgccgg gcgcagtctg gccggcggcg cggtggccgg
      541 ctgggtgggg ccgcagttgg cctactatgg cgactcgctg ctggccgccg cccggcacta
      601 tggcttggcg ctcaccctcg acacgcccat ccgcgactac agcccggcgg cgcgcgacct
      661 gttcttcttc ggcgtggaga gtccccagtt ccgccgccac ttcccggaca gagagccgcc
      721 ggccaccgtc cgcggcgggc gcttcgaggg cctggccacg gcggcgctgc gccgctatgg
      781 cgagcacgcc ggcgacgagg actacgccgc caggatggcc ggccaggtcg tggccgctgt
      841 ctgccccgat tgcctggggc agcgcctgcg gccggagagc cgcgccgtca ccgtcgccgg
      901 gcaaacgatc atggccgtgg ccgggatgtc gctggcggcg ctggcggagt ggctgcgcga
      961 gctgctggcg gcgctctcgg ccgacgagca tcgcatcgcc gcgccgctgc tcgaggacct
     1021 gaacgcgcgc ctgggccggc tgctggaggt cggcgtcggc tacctgacga tggcgcgcgc
     1081 ttcggcgacg ctctcggccg gcgaggcgca gcggctgcgg ctggcgtcgc tggtcggctc
     1141 cgggctgacc gggatgctct acatcctcga cgagccaacc gtcggcctgc acccacgcga
     1201 cacggagcgg ctgctgcgcg tgctgcgccg cctgcgcgac ctgggcaaca ccgtcctcgt
     1261 cgtcgagcac gacctggacg tgatccgcgc cgcgaataca gtcatcgaca tggggccggg
     1321 ggcgggcagg cacggcggcc gggtcgtcgc cgcgggcacg ccggccgaag tggccgcgca
     1381 cccaacctcc ctcaccggcg cgtacctggc ggggcgggcg gccacgcccc cgccgccgcg
     1441 ccggccgccg gccgaacggg cgctgaccat cgccggggcg cgcgccaaca acctgcgcaa
     1501 cattaccgcg cggctgccac tggagcggtt ggtggttgtc accggcgtca gtggcgcggg
     1561 caagtcgtcg ctggtgttcg acatcctcga ccgggccggg cgggcgcgct tctatggcgc
     1621 gaccgacacc cccggcgagc acgacgccat cgcgggctgg gaggccatcg acaagctggt
     1681 caccatcggc caggaaccca tcggccgccg gccgcgctcc aacgccgcca cctacgccga
     1741 cgtcttcacg cccatccgcg agctgttcgc ggcgcagccg gaagcgaagc agcgcggctt
     1801 cacggcgcgg cgcttttcgt tcaacgtgcc cggcggccgc tgcgagcgct gcgaaggggc
     1861 gggggtgctg ccggtgacga tgcacctgct gccaccggtg gacgtgcgct gcccggtctg
     1921 ccacggccgc cgctacgacg cggcgacgct ggccgtcacc tatggcggcc acgacatcgc
     1981 cgcggtgctg gccatgactg tcgaggaggc gcttgcgcta ttcgccggcg tgcccgccgt
     2041 ggccacgcgg ctggcggtga tggccgacgt gggcctgggc tatctgccgc tgggccagcc
     2101 ggcgacgacc ctctccggcg gcgaggccca gcgcgtcaag ctggcccgcg agctggggcg
     2161 gcgcggcggc ggccacacgc tctacctgct cgacgagcca acgaccggcc tgcacccggc
     2221 cgacacggcc cggctgctgg ccgtgctcaa ccggctggtg gacgccggcc acagcgtcgt
     2281 cgtcgtggag cacaacctcg acgtgattcg ggcagctgac tgggtgatcg acctggggcc
     2341 ggagggcggc gcggccggcg gcgagctgat cgccgcgggc acgccagaag acgtggccgc
     2401 cagcccgcgc tcgttgaccg gggcgttcct gcggccgctg ctgtcgtaat ttctgtctcc
     2461 agcggtattg ccagggcgga agaagatctc acgcaaagcc gcaaagccgc aaagaaaaaa
     2521 gattcttctt tgcgtctctg cgtctttgcg tgagatcctt cgtccctcaa ttgcccccta
     2581 gttgtcatta atgggcaaac tggggtcaaa aatgataact actttacact tctcaggcac
     2641 tcttgtgcta tacttcattg gctcgttcgt tcacagctgc ccatcccagg caacactgct
     2701 gcaaacccac cgagcgctgg cagtccagtc tcggcttact gcgtgaggtg tggcgatgat
     2761 ggaaaaaatc ctctaacata accccggctt cggccatccc gtgctctcgc ccgctttcgc
     2821 gggtctgttc gccctgccgc gaaccgttgc gcttctattt cccggcccct catttcggcc
     2881 actatcaaca ataccgcccc ggcgcgctgt tgcctctggc ggccatacgt gagtgtcact
     2941 atgacaaccg atcttgatct gaagacaacg ctgtccgcgc gtcgtatcgt cggcctctgg
     3001 cgcatgatca ctggctaccg ggccatctac gtcggtgcgt tcctgtgcat cgggttggcg
     3061 gccgtttgcc agacattgtt ctacttcctg ttgcgctact ttaccgacga cgtgctcggc
     3121 atcgaggcca tgcgcgggca gttggcctgg gtggccgccg gcttcatcgg cctggcgctg
     3181 gctcaggggc tgttcacgtt catgggcggc cgctgggcga ccatgacgtc ggagaatgtc
     3241 atccgccgcc tgcgcgacta tctgtatgac cacgtgcagc ggctgacctt tgcctaccac
     3301 gacaagacgc ccaccggcga cctcatccag cgctccacat ccgatgtgga gacgatgcgg
     3361 cgcttcctgg gcgaggagtc ggtcggcctc ggccgtatcc tgtcgctctt cgtggtcaac
     3421 ttcgtcggcc tgctgacgct ggatacgcgc ctggcgctca tctccatcgt cgtcgtgccg
     3481 gtcatcgtcg ttgtgtcgat ctacttcttc cgcatgatcg gcaagcgcta cgagatcttc
     3541 caggaagaag aggccaagct atcgacgacg ctgcaagaga acctgagcgg cgtgcgcgtc
     3601 gtgcgcgcct ttgcccggca ggacttcgag cggcagaagt tcgaggtcac caaccgcggc
     3661 cggtacgaca gcggccggaa gctgctggtg ctccatgccg tctactggcc atcgaccgac
     3721 atcatcgttg gcatccagct gatcatcggc tatgccgtcg gcgcgacgat ggccatcaac
     3781 ggcgcgatca ccgtgggcac gttcctggcc tacatgggta tgctgacgtg gatcatgtgg
     3841 cccatccgca acatcggccg gctcatcgtg cagatgtcga tggccctggt gtcgttcgac
     3901 cgcgtgtcgg agatcatccg cgtcgaccgc gaaccgttgg acgtgggcac ccatcgtccc
     3961 gacgggccgc cgcgcggtga gatcgtcttc gacgacgtgc gcttcgccta cgccgaggac
     4021 atggccgtgc tcaatggcat caccttccgc gccgagccgg ggcaggtcgt cgccctggtc
     4081 gggccgacgg gatcgggtaa gacctcgctg gtcaacctgt tgccgcgctt ctatgaatac
     4141 cagggcagca tccgcctgga cggcgtggag ctgcgcgact acacgcgccg ctacctgcgc
     4201 gagc
//
LOCUS       DAOSMQ010000273         4195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21250_length_4195_cov_3.909179, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000273 DAOSMQ010000000
VERSION     DAOSMQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21250_length_4195_cov_3.909179"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            515..1159
                     /locus_tag="PK829_09630"
     CDS             515..1159
                     /locus_tag="PK829_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU41516.1"
                     /translation="MPNDAPALSLLLIEDQTLMRQGLRTLLDLEPGMHVAGEAADGAN
                     GVRLALELRPDVVLMDVQMPGLNGVEATAALCAAWPAARVVILTTFDRDDYVFQAIRA
                     GAMGYLLKDAPAEELLRTIRRVAAGEAFIQPEIASRTLRELLRPKEAPLEPLSERERE
                     VLVLLAQGASNRDIADRLVISEGTVKNHVSSILGKLQAENRTQAAELARRYGLM"
     gene            1218..2453
                     /locus_tag="PK829_09635"
     CDS             1218..2453
                     /locus_tag="PK829_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOU41517.1"
                     /translation="MTPEFDRALGRYAELIVKVGLNLQPGQRLLIRAPIEAAPLARAV
                     AAQAYDRGARLVETLYNDPQMALVRFGHAPADSFTEVSQWPTATGLEYAGNGQAMLSI
                     VGTDPDLLRDVDPARLNTVRRAEGQALAPVSRLVSADAINWCVVGYATPAWAARVFPE
                     LSPEEATTRLWAAIFAAVRLDAPDPLAAWQAHNAVLAARTAALNERQYTALHFTGPGT
                     DLTIGLPVNHRWIGGAAPTAIGISNVANIPTEEIFTAPHRGRVEGTVRATMPLSLSGK
                     LVKDFAVTFAGGRVVDVRAAAGEALLRSLIETDEGAARLGEVALVPAGSPISQSGILY
                     YNTLFDENAASHIALGRAYRNCIAGCEGLDDDDFGALGGNSSNAHVDFMIGSAEIDVD
                     GITGDGAAEPLMRAGEWVD"
     assembly_gap    2689..2788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2908..>4195)
                     /locus_tag="PK829_09640"
     CDS             complement(2908..>4195)
                     /locus_tag="PK829_09640"
                     /inference="COORDINATES: protein motif:HMM:NF021158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HOU41518.1"
                     /translation="IEVELQNVSGGVPAVVAATTVNADGQQVPIPFDLPYDATAIDPA
                     GTYLLYARIRVNDAVTWATPTGVPVLTNGAATGNVELIVSPVAATTGGSIQGTVTTPR
                     SPAALPAGAVLQVELREPMLADAPAAAVVEVPLDGVNFPVSFALSYDPATIAADRSYV
                     AAARVVAGDRLLYATLSPVPVLTGGAPASAIVVPVAEVPDPAGGVLRFTVTSDTAASW
                     PAGSNAYLNVEIREPNLADAPALAFTYVPLAGLTLPVAWELGYPLASIDANKAYVLDA
                     RIIDNNALTYQATTPLPVLTQGAPANDVSLALTAQAAPPTGGTGGNEGVITGVITTDA
                     PTALDPAATYFIDLREAGTAGDPIVTISAALEGQQFPIPFEVPYVPAEIDPAKSYVVG
                     ARILLGDQVLYASAVGVPVLTQGAGTSDIVVNIPPQ"
BASE COUNT          671 a         1432 c         1402 g          590 t
ORIGIN      
        1 ggttctacaa gatcgcgact gttcagttag gcaagcaaaa gcattcctcc tacccgcaac
       61 ccccctgaag gtgcggtgta gtgtccagta ctcgatccct acctgtcgtg ccattgtaag
      121 gcgtgataga cagaatctga tagtgacata tgacatgaat tcgacatgac acctgtcatg
      181 gctcgaccag gttgggaatg cacagcgaca cagtaacgtt ctggctatta gtatcgtaag
      241 gaaggatgct tacagtctac attacagctc gataagaaac gccacaaacg acaacggcac
      301 gcgaaattga tcccgccagc ggccggcccg cgccggccgc tgtgccctcg cgccccgccg
      361 gctaggaccg acgacctgac agggggcggc caaactctct ctgactggta agccagttga
      421 ggccgccccc ctgtccggcc tacacgctcg aaacgacttt ggaaaagccc tggccaagat
      481 tgttagcacc gccgcccacg ggtacaatcc gcccatgccc aacgacgcgc ccgcgctgtc
      541 cctgctgctc atcgaagacc agaccctcat gcgtcagggc ctgcgcacgc tgctcgacct
      601 ggagccgggc atgcacgtcg ccggcgaggc ggccgatggc gcgaacggcg tgcggctggc
      661 gctggagttg cgccccgacg tggtgctgat ggacgtgcag atgcccggcc tgaacggcgt
      721 cgaggccacg gccgccctct gcgccgcctg gccggccgcc cgcgtcgtca tcctgaccac
      781 cttcgaccgc gacgactacg ttttccaggc cattcgcgcc ggggccatgg gctacctgct
      841 gaaggacgcc ccggccgagg aactgctgcg caccattcgc cgcgtggccg ccggcgaggc
      901 tttcatccag ccggagatcg cctcgcggac gctgcgcgag ctgctgcggc cgaaggaagc
      961 gccgctggag ccgctgtcgg agcgcgagcg cgaggtgctg gtgctgctgg cccagggcgc
     1021 gtccaaccgc gacattgccg accgcctggt catcagcgag ggcacggtca aaaaccacgt
     1081 ctccagtatc ctcggcaagc tccaggccga gaaccgcacc caggcggccg agctggcccg
     1141 ccgctatggg ctgatgtagg cgcgcccgcc ccgacggggg cgggcgccga tctggaataa
     1201 tccacttgga gaaccatatg acccctgaat tcgaccgcgc cctcggccgc tatgccgagc
     1261 tgatcgtcaa agtcggcctg aacctgcaac ccggccaacg gctgttgatt cgcgcgccca
     1321 tcgaggccgc gcctctggcc cgcgccgtcg ccgcccaggc ctatgaccgc ggcgcgcgcc
     1381 tggtggagac gctctacaat gacccccaga tggcgctcgt ccgcttcggc cacgccccgg
     1441 ccgactcctt caccgaagtc agccagtggc ccacggccac cgggctggag tacgccggca
     1501 acggccaggc gatgctgtcc atcgtcggca ccgaccccga cctgctgcgc gatgtcgatc
     1561 cggcccggct caacaccgtc cgccgcgccg agggccaggc cttagcgccc gtttcccggc
     1621 tggtcagcgc cgacgcgatc aactggtgcg tcgtcggcta cgccacgccg gcctgggcgg
     1681 cgcgcgtctt cccggaactg tcgccggagg aggcgaccac gcgcctgtgg gcggccatct
     1741 tcgccgccgt ccgcctcgac gcgcccgacc cgctggccgc ctggcaggcc cacaacgccg
     1801 tcctggccgc gcgcacggcg gcgctgaacg agcgccagta caccgccctg cacttcaccg
     1861 gccccggcac cgacctgacc atcggcctgc ccgtcaacca ccgctggatc ggcggcgcgg
     1921 ccccgacggc catcggcatc agcaacgtgg ccaacatccc cacggaggag atcttcaccg
     1981 ccccccaccg cggccgcgtc gagggcacgg tgcgagcgac aatgccgctg agcctgagcg
     2041 gtaagctggt gaaggacttc gcggtgacgt tcgccggcgg ccgggtggtg gacgtgcgcg
     2101 ccgccgcggg cgaggcgctg ctgcggtcgc tcatcgagac cgacgagggc gcggcgcggc
     2161 tgggcgaggt cgccctggtg ccggccggct cgcccatctc gcaatcgggc atcctctact
     2221 acaacaccct cttcgacgag aacgccgcca gccacatcgc tctcggccgc gcctatcgca
     2281 actgcattgc tggctgcgaa gggctagacg acgacgactt cggcgcgctg ggcggcaaca
     2341 gcagcaacgc ccatgtggac ttcatgatcg gctcggccga aatcgacgtc gatggcatca
     2401 ccggcgacgg cgcggccgag ccgctgatgc gggcggggga gtgggtggat tgagccgaag
     2461 aaggttccag gggccagggg ccaggggcca ggggccaggg gcagaaaggt tccaggttcc
     2521 ggggtagggg cgggtctgag acccgccacc aacgacaccc atcctgtagg ggcgggtctg
     2581 agacccgccc tcaagcatag ccaaagtccg ggggaagggg ccagaagaag gttccagggg
     2641 ccaggggcca ggggcagaaa ggttccaggt tccggggtag gggcgggtnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ggcgggtctg agacccgccc tcaagcatag
     2821 ccaaagtccg ggggaagggg ccacgcccag cgaagcaccc tcttcccctg gaacctggaa
     2881 cctggaacct ggccccttct tcccccccta ctgcggcggg atattcacga caatgtcgct
     2941 cgtccccgcg ccctgggtca gaaccggcac gcccacggcc gaggcgtaga gcacctgatc
     3001 gcccagcaaa atgcgcgcgc cgacgacgta gctcttggcg gggtcaatct ccgcgggtac
     3061 atagggcacc tcgaacggga tggggaactg ctgcccctcc agcgccgccg agatggtcac
     3121 aatcgggtcg ccggccgtgc ccgcctcgcg caggtcgatg aagtaggtcg ccgccgggtc
     3181 cagggccgtg ggggcgtcgg tggtgatgac gccggtgatg acgccctcgt tgccgcccgt
     3241 gccgcccgtg gggggggccg cttgcgccgt cagggccagg ctgacatcgt tggccggcgc
     3301 gccctgggtc agcaccggca ggggcgtggt tgcctggtag gtcagcgcat tgttgtcgat
     3361 gatgcgcgcg tcgaggacgt aggccttatt ggcgtcgata ctggccaacg gatagcccaa
     3421 ctcccaggcc acgggcagcg ttagaccggc caggggcacg taggtaaagg ccagggccgg
     3481 cgcgtcggcc aggtttggct cgcggatttc cacgttcagg taggcgttgg agccggccgg
     3541 ccagctggcg gccgtgtcgc tcgtcaccgt gaagcgcagc acgccgccgg ccgggtcggg
     3601 cacttcggcc acgggcacga cgatggcgct ggccggcgcg ccgccggtca gcaccggcac
     3661 gggcgacagc gtggcataga gcagccggtc gccggccacc acccgcgccg cggcgacata
     3721 gctgcggtcg gcggcgatgg tcgccgggtc ataggacagg gcaaatgaga cggggaaatt
     3781 caccccgtcc agcggcactt cgacgacggc cgcggcgggg gcgtcggcca gcatcggctc
     3841 gcgcagctcc acctggagca cagcgccggc cggcagcgcc gccggcgagc gcggcgtcgt
     3901 caccgtcccc tggatgctgc cgccggttgt cgccgccacc gggctaacga tcagttcgac
     3961 attgcccgtc gccgcgccgt tggtcagcac gggcacgccc gtcggcgtgg cccaggtgac
     4021 ggcgtcgtta acgcggatgc gggcgtagag caggtacgtg ccggccgggt cgatggccgt
     4081 ggcgtcatag ggcagatcga aggggatggg cacttgttgc ccgtcggcgt tgacggtcgt
     4141 cgcggcgacc accgccggca caccgccgga aacgttctgc aactccacct cgatg
//
LOCUS       DAOSMQ010000274         4184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21332_length_4184_cov_4.263018, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000274 DAOSMQ010000000
VERSION     DAOSMQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21332_length_4184_cov_4.263018"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK829_09645"
     CDS             complement(<1..538)
                     /locus_tag="PK829_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOU41519.1"
                     /translation="MTAEPVVLNGRYRLIDRIGSGGMSVVYRAQDLSLGRIVAIKVLN
                     ENLTDDEGFLRRFQREAHAAANLSHPNIVTVHDIGQDGDRHYIVMEYIDGRTVKQLVR
                     LQNQQGQPLPVSRVLDLAIQICAGIGYAHRANLVHCDVKSQNILVTRDDRVKVADFGI
                     ARALSEATQHTSDSQIWGT"
     gene            complement(595..2922)
                     /locus_tag="PK829_09650"
     CDS             complement(595..2922)
                     /locus_tag="PK829_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOU41520.1"
                     /translation="MNARVRLLSIGGLLLLLGALLFVRQPSSSAVLYTGVGLVDSGPL
                     HLEVVVDPPVARPGDTVRLTARVSSRAAGRLTPSITLRLPRGLSGDVYALPAGATLNL
                     QDNRIDWQPVVGGGQTQEFSLDVTVETADVLTPEQSVIAQIRHQGDTQSAEAQIWLGI
                     PPLIGEMLPQHEAAVGQPIQLQAQVAGPGPLSAEWDLGDGRRLDLADPVVVFPAAGQY
                     DVTLRVSNPAGAVTRRATVDILPNPVASFQPDDDAPAIAQPVTFLNASGGQPPLTVFW
                     DFGDGTTLMGEQQPSHTYQQGGVYRVRLTVENSFGRSEAVWDVTVGNAPVVEMSLAEG
                     AAVGRPLTGQASTTDGASRFLWDMGDGRRHEGATVSHLYRWPGDYYVTLVADNGFGQA
                     QVGRWIHVDAGLTTLFLPLAAYQNGSGVANLSAEAALPPELDPVTTTPGQAYSLPQLT
                     FPTGTTAAEELYTYLNAARAQFALPPLDYNQTLSAAAQGHAQDKAQFPANPHVGSDGT
                     TSAERFLRVGYGGGFAGEATAWGFADARLAVEFWMNSDSHRALVLNRLATDVGVGYVV
                     DYGTENIWHWTAEFGVSYGAPVRPVVRLQEPAPELSTLDDETVNYSWLWPQPLAAGER
                     FTIYVLAGNQSVALGSVAAPVYGSRYILSADALSTLGPAGLSPTTYRWLVRLEDGLGH
                     TLAESEQRAIAFAADPDAPTPMPTVAIITATPVGPTSTPSPTIPPTAAAPTVEPPPVV
                     VTATPPPPTAEPTQPAEPTPGLVTATPPPSPTAEP"
     gene            complement(3295..3729)
                     /locus_tag="PK829_09655"
     CDS             complement(3295..3729)
                     /locus_tag="PK829_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOU41521.1"
                     /translation="MRSFGSGSEFRKGVEYIEGIGPAFGEKLRAAGVVTVLDLMVEGA
                     TRMGRKHLSERSGLSASQILTWVNHIDLFRIKGVAEEYADLLEQSGVDTVVELAQRNP
                     NNLFKRMIDINEQKQLVRRTPHLSDVQSWVEQAKGLKRLIHY"
BASE COUNT          674 a         1373 c         1446 g          691 t
ORIGIN      
        1 gcgtgcccca gatttggctg tcggaggtgt gctgcgtcgc ctcgctcagg gcgcgagcga
       61 tgccaaagtc ggccaccttc acgcggtcgt cccgcgtcac aaggatgttc tgcgacttga
      121 cgtcacaatg taccaggttg gcccggtggg cgtagccgat gccggcgcaa atctggatgg
      181 ccaggtcaag gacacggcta acgggcagcg gctgaccctg ctggttctgc agcctgacca
      241 actgcttcac cgtccgcccg tcaatatact ccatgacgat gtagtgccgg tcgccgtcct
      301 ggccaatgtc gtggacagtg acgatattgg ggtgggagag gttggccgcg gcgtgggctt
      361 cgcgctggaa gcgacgcagg aagccctcat cgtcggtcag gttctcattc agcaccttga
      421 tcgcgacaat gcggcccaag gagaggtcct gcgcgcgata gacgaccgac atgccgccac
      481 taccgatccg atcgataaga cgatagcggc cgttgaggac aaccggctct gctgtcatgc
      541 catgattgta aagcggagcg gtctcactcg caaggacgct ggccggtatg tcatctacgg
      601 ctcagccgtg ggcgaaggtg gcggcgtggc ggtgaccagg ccgggggtcg gctcggccgg
      661 ctgggtgggt tcggccgtcg gcggcggggg cgtggccgtg acgacaaccg gcggcggttc
      721 gacagtgggc gcggccgccg tcggcgggat ggtcggcgat ggcgtgctcg ttggcccgac
      781 tggcgtcgct gtgatgatgg ctacggtggg catgggtgtg ggggcgtccg ggtcggcggc
      841 aaaggcgata gcgcgctgct cgctttcggc caacgtgtgg cccaacccgt cctccagccg
      901 caccagccag cggtaagttg tcggcgacag cccggccggc cccagcgtgc ttagcgcgtc
      961 ggccgacagg atgtagcggc tgccatagac gggcgcggcg acactaccca gggccacgct
     1021 ttggttgcca gccagcacgt aaatggtgaa gcgctcgccc gcggccagcg gctgtggcca
     1081 gagccagctg tagttgaccg tctcgtcatc gagggtgctc agttcagggg ccggttcttg
     1141 caggcgcacg accgggcgca cgggcgcgcc gtagctgacg ccgaattcgg ctgtccaatg
     1201 ccagatgttc tccgtgccgt agtcaaccac ataaccaacg cccacatctg tggccaggcg
     1261 attgagcacc agcgcccggt ggctgtcgct gttcatccag aactcgacgg ccagacgcgc
     1321 gtcggcgaag ccccaggccg tagcctcacc ggcgaagccg ccgccgtagc caacacgcag
     1381 gaagcgctcg gctgaggtcg tgccgtcgga gccgacgtgg gggttggctg ggaattgcgc
     1441 cttgtcctgg gcgtggccct gggccgcggc gctcagcgtc tggttatagt ccagcggtgg
     1501 cagggcaaac tgggcgcggg cggcgttgag gtaggtgtaa agctcctcgg ccgcggtcgt
     1561 gcccgtgggg aacgtcagct gcgggaggct gtaggcctgg cccggcgtcg ttgtaacggg
     1621 gtcaagctcg ggcggtaggg cagcctcagc gctcaggttg gccacgccgc tgccgttctg
     1681 gtaggcggcc agcggcagga aaagcgtcgt caggccggcg tcgacgtgaa tccagcggcc
     1741 gacctgcgcc tggccgaagc cattgtcggc caccagcgtc acgtagtagt cgcccggcca
     1801 ccggtagagg tggctgacag tggctccttc gtggcggcga ccgtcgccca tgtcccaaag
     1861 aaagcgcgac gcgccgtctg tcgtagacgc ctgcccggtc agcggccggc caacggccgc
     1921 gccctcggcg agcgacatct cgaccaccgg cgcgttgcca acggtcacat cccagacggc
     1981 ctcggaacgg ccgaagctgt tctcaactgt tagccggacg cgatagacgc cgccctgctg
     2041 gtaggtgtgg ctgggctgtt gctcgcccat gagcgtcgtc ccgtcgccga agtcccagaa
     2101 gacggtcagg ggtggctggc caccactggc gttgagaaag gtcaccggct gggcgatggc
     2161 cggagcgtca tcgtcgggct ggaagctggc tacggggttg ggcaggatgt ccacggtcgc
     2221 ccgccgggtg accgcgccgg caggattgct gacgcgcagg gtgacatcat actgcccggc
     2281 tgcgggaaag acgacgacgg ggtcggccag gtcaaggcgg cggccgtcgc ccaggtccca
     2341 ctctgcgctc agcggccccg gcccggcgac ctgggcctgt agctggatcg gctggccgac
     2401 agcggcctca tgctgcggca gcatctcgcc gatcagtggt gggatgccaa gccagatttg
     2461 cgcttcagcc gactgggtgt cgccctggtg tcgtatctgg gcaatgacgc tctgctcggg
     2521 ggtcaatacg tcagcggtct cgacggtcac atcaaggcta aactcttgcg tctgcccacc
     2581 gccgacgacg ggctgccagt cgatacggtt gtcctggagg ttgagggtcg cccccgcggg
     2641 cagggcatat acgtcgccgc tcaggccgcg cggcaggcgc aaggtgatcg acggcgtcag
     2701 ccggccggcg gcgcggctgc tgacgcgggc tgtcaggcgc acggtatcgc ccggccgggc
     2761 caccggggga tcgacgacaa cttccaggtg gagcgggccg ctgtctacca ggccgacgcc
     2821 cgtatagagg acggccgagg aggatggctg ccgcacgaag agtaacgcgc ccagcaacag
     2881 cagcaatccc ccgatcgaca gcagtcgaac cctggcattc attgtggcgg cagtttaaca
     2941 gagaggaggt aatcgcacaa gtgcgcccag ggctttgcca aagtcacccc aggcgcaccg
     3001 acctgacagg tgggcggccg cgattgggac tgccaccaca gaacgactgg ccgcctaccg
     3061 gtcaggtcaa tggtcactcg acgactctgg caaagccctg caagtgcgcc aggcatgccc
     3121 gccacgtgcc gaggacgact tgacgaaggg gattggcacg cagatagagt ggcccgccgc
     3181 taacgccgat tgacgcagat cagagccatt gagattccaa tcggcgtcaa tctgcccaac
     3241 cggcgttagc ggcgtgcgtt ttggcgacgc cgggccgaag cccggaaggt gctcctagta
     3301 gtggatcagc cgcttcaggc ccttagcctg ctcgacccag ctctgcacat cagatagatg
     3361 tggcgtgcgc cggacgagtt gcttctgctc gttgatgtcg atcatgcgct tgaacaggtt
     3421 gttaggattg cgctgggcca actcgacgac tgtatctacg cccgactgct ccagcagatc
     3481 ggcgtactcc tcggccacgc ccttgatgcg gaagaggtcg atgtggttca cccaggtgag
     3541 aatctggctg gcgctcaggc cgctgcgctc ggacaggtgc ttccggccca tccgggtggc
     3601 tccttcgacc atcaagtcca gcaccgtgac gacaccggcg gcgcgcagct tctcgccgaa
     3661 cgccgggccg atgccctcga tgtactcaac gcccttgcga aactccgagc cagagccgaa
     3721 cgagcgcagg tattcctcta gttccgagcg caactgggcg gccgacggct cggcgggcgc
     3781 ggcgcgctcg tccatgctca tcgcgccggc agtagcgcca gcggtcgcgc ccatcgccat
     3841 gccggcgtcg ctcatggcct cgccggtggc atcgaccgca tcgccggcag cgtcgcccat
     3901 gtcctcgccc cggtcgccaa cccacgcgcc ggcatcgctc atggcctcgc cggtggcatc
     3961 gaccgcatcg ccggcggcgt cgcccatgtc ctcgacccgg tcgccaaccc acgcgccggc
     4021 gtcgctcatc gcctcgccgg tggcatcgac cgcatcgccg gcagcgtcgc ccatgtcctc
     4081 gacccggtcg ccaacccagt cgccggcgtc gctcatcgcc tcgccggtag catcgaccgc
     4141 atcaccagtg gcgtcgccgg catcgtcgag cttgtcgcca accc
//
LOCUS       DAOSMQ010000275         4180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21346_length_4180_cov_4.347394, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000275 DAOSMQ010000000
VERSION     DAOSMQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21346_length_4180_cov_4.347394"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PK829_09660"
     CDS             complement(<1..1349)
                     /locus_tag="PK829_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOU41522.1"
                     /translation="MSIEPTLRPFFDPAGVAIIGASSAPAKLGFGVARNLTQSGYTGA
                     IHFVNPRGGRLLERPVYTTIAAVPDPVDLAVVIVPAPAVAETLAQAGDRGIRAAIIVS
                     GGFRETGPAGARLEDDCLRVARNYDMRLIGPNCVGLLDTHRPLDTTFLPPPGPTPGDV
                     AFISHSGAICAAVIDWARGQGFGLSQLVSLGNQIDVRETDMLTSVAADRHTRVITLYL
                     EGISDGRRFVHCARQVTRHKPIIALKVGRFAAGQRAVASHTGALAGQESAFNAAFRRA
                     GVIRANTSEELFDWARALAWCPLPAGRDVAVLTNAGGPGVTAADMLESRGLRLAALRP
                     ETDAALRALLPPDAGLANPVDMLAAATAAQYAGALRLLLDDPGVHSAMVVVPPPPMES
                     AGSIARALIPIITTAAKPVVIALMGERMIQEAVEYFRAARVPEYRFPERAAAALAVLV
                     "
     gene            complement(1356..1844)
                     /locus_tag="PK829_09665"
     CDS             complement(1356..1844)
                     /locus_tag="PK829_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOU41523.1"
                     /translation="MNAALIVLDAQNDFFAPDNPNLPAFEATVPVINGAIAHFRAQGA
                     PPLFVQHTTERKPVGSQPWAINDRFDCRPTDTRMNKTGENAFWNTGLDTLLRSQRVGQ
                     VIIAGYISERCVLSTLRGAAERGYEAVLLDGGIATFDDRYTQFVLEISPRVALEALRA
                     GL"
     gene            complement(1841..2257)
                     /locus_tag="PK829_09670"
     CDS             complement(1841..2257)
                     /locus_tag="PK829_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOU41524.1"
                     /translation="MAELKTRENDGDVLAFLNAVPDESKRADALRMMAIMREVTGEEP
                     RLWGDNIVGYGHYHYKYASGREGDWFLAGFSPRKANLTLYIIAGFEQFEELRARLGKH
                     TVGKSCLYVKRLSDLDEATLRELIGRSVAHMRATNP"
     gene            complement(2600..>4180)
                     /locus_tag="PK829_09675"
     CDS             complement(2600..>4180)
                     /locus_tag="PK829_09675"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41525.1"
                     /translation="GDFEGATALTITVPAGTLPEHSAVTLELSRSPAGSLLNGLEYLT
                     GFPYGCVEQTMSRALPNVAVDRAFDALGAPQPEGLELGRLVNESAQRLYGFQHDDGGW
                     GWWFDDDSTPYQTAWVVFGLATMVEAGHEIDPAVIARGATYLNDSLDKADNRTRAMML
                     YSLAVAGQPNGAAALELAALPDDDLDAFSLAALALALDAAGEADAAQAVMARLAAQAI
                     VADGRAHWGVGGNDGQYRSKTMASATRSTALALRAFVRLRPGDPLEAPAVRWLMAQRR
                     GLGWGTTNETAYTILAVADHIEASGLGETALSASVALDGAPPINTTLAAGELSATLTL
                     PAEALTPGAHSLTVSGDSRVFYTLHEDYAVGQAQIDADGPLAVSRQFLTPAGKPLGEV
                     HVGDLVLVRLRVNVPGPTYFVLVEDRPVAGLEPLNEHLNTTSRAAGYTSNTGDYYSYF
                     DGGFSWSKLGYNYKEIRDGRVTFFISTLTRSTSFDYLARATHSGDFSALPAEAWAMYE
                     LDTWGRSASDTLAILAER"
BASE COUNT          597 a         1320 c         1627 g          636 t
ORIGIN      
        1 accagcacgg ccagcgccgc cgcggcccgc tccgggaagc gatactccgg cacgcgggcg
       61 gcgcggaagt actcgaccgc ctcctggatc atgcgctcgc ccatgagagc gatgacgacc
      121 ggcttggccg ccgtggtgat gatgggaatg agggcgcggg cgatgctgcc ggccgattcc
      181 atcggcggcg gcgggacgac gaccatggcg ctatggacgc cgggatcgtc gagcagcagc
      241 cgcagcgccc cggcgtattg ggccgccgtg gctgcggcca gcatgtccac ggggttggcc
      301 aggccggcgt cgggcggcaa cagggcgcgc agggcggcgt cggtttccgg ccgcagcgcg
      361 gccagccgca ggccgcggct ttccagcatg tcggccgcgg tcacgccggg gccgccggca
      421 ttggtcagca cggccacgtc gcgcccggct ggcagcgggc accaggccag cgcccgcgcc
      481 cagtcgaaca gctcctcgct ggtgttggcg cggatgacgc cggcgcggcg gaaggcggcg
      541 ttgaaggccg actcctggcc ggccagcgcg ccggtgtggc tggccacggc ccgctgcccg
      601 gcggcgaaac ggccgacctt gagggcgatg atgggcttgt ggcgcgtcac ctgccgggcg
      661 cagtggacga agcggcggcc gtcgctgatg ccctccaggt agagggtgat gacgcgcgtg
      721 tgcctgtcgg cggccacgga ggtcagcatg tcggtctcgc gcacgtcgat ctggttgccc
      781 aggctgacca gttgcgacag gccgaagccc tggccgcgcg cccagtcgat gacggcggcg
      841 cagatggccc cggagtggga gatgaaggct acgtcgccgg gggtggggcc gggcgggggc
      901 aggaaggtgg tatcgagcgg ccggtgggtg tccagcaggc cgacgcagtt ggggccgatg
      961 aggcgcatgt cgtagttgcg ggcgacgcgc agacagtcgt cctccaggcg cgcgccggcc
     1021 ggccccgtct cgcggaagcc gccggagacg ataatggccg cgcggatgcc gcggtcgccg
     1081 gcctgggcca gcgtctcggc cacggccggc gcgggcacga tgaccacggc caggtcaacc
     1141 gggtcgggca cggcggcgat ggtggtatag acggggcgtt ccagcaggcg gccgccgcgc
     1201 gggttgacga agtggatcgc gccggtatag ccgctctggg tcaggttgcg ggcgacgccg
     1261 aagccgagct tggccggggc gctgctggcc ccgatgatgg ccacgccggc gggatcgaag
     1321 aaggggcgga gggtgggttc gatggacatg ggggactata gcccggcgcg cagcgcttcc
     1381 agcgcgacgc ggggggaaat ctccaggaca aattgggtgt agcggtcgtc gaaggtggcg
     1441 atgccgccat cgagcagaac ggcctcgtag ccgcgctcgg ccgcgccgcg cagcgtggac
     1501 aggacgcagc gctcggagat gtagccggcg atgatcacct ggccgacgcg ctgggagcgc
     1561 agcagcgtgt ccaggccggt gttccagaag gcgttctcgc cggtcttgtt catgcgcgtg
     1621 tcggtggggc ggcagtcgaa gcggtcgttg atcgcccacg gctgcgagcc gacgggcttg
     1681 cgctccgtcg tgtgctggac gaagaggggc ggcgcgccct gtgcccggaa gtgggcgatc
     1741 gcgccgttga tgaccggcac ggtggcctcg aaggcgggca agttggggtt gtccggcgcg
     1801 aagaagtcgt tttgcgcgtc gaggacgatc agggcggcgt tcacgggttg gtggcgcgca
     1861 tgtgggcgac ggagcggccg atgagttcgc gcagggtcgc ctcgtcgagg tcggacagcc
     1921 gcttgacata aagacacgac ttgccgacgg tgtgcttgcc cagccgggcg cgcaactcct
     1981 cgaactgctc gaagccggca atgatgtaga gggtcaggtt ggctttgcgc ggcgagaagc
     2041 cggccaggaa ccagtcgccc tcgcggccgc tggcgtactt gtagtggtag tggccatagc
     2101 caacgatgtt gtcgccccac aggcgcggct cctcgccggt cacttcgcgc atgatcgcca
     2161 tcatgcgcag ggcatcggcc cgtttgctct cgtcgggcac ggcattgagg aaggccaata
     2221 cgtcgccatc gttttctctg gtctttagtt cagccatggg cgttctcctg tcgggtctga
     2281 gatgggatta taggggctgg gggcgggagg ggcaaagaac ggtggggccg ggtcgttgcc
     2341 aagtcggtag gcggtcacgt aaaggcgctc acgcaaaggc gcgaaggggc aaaaggcgcc
     2401 aagaggagac ggtggggccg ggtcgttgcc aagtcggtag gcgcttacgt aaaggcgctc
     2461 acgcaaaggc gcaaagtaag cagaggcgca aagaagaagc gcgatcatcg accgtttctt
     2521 ctttgcgcct tttgcccctt cgcgcctttg cgtgagcggc ttggcgtgag cggcaagggg
     2581 ccaacacgca ggttcgcccc taccgctcgg ccaggatcgc cagtgtgtcg ctggccgaac
     2641 gcccccaggt gtccagttcg tacatggccc aggcttcggc cggcagggcg ctgaagtcgc
     2701 cgctgtgggt ggcgcgggcc aggtagtcga agctggtgga gcgggtgagc gtgctgatga
     2761 agaacgtcac ccggccgtcg cggatctctt tgtagttgta gcctaatttc gaccacgaga
     2821 agccgccgtc aaagtaggaa tagtagtcgc cagtgttgct ggtatagccg gcggcgcggc
     2881 tggtggtgtt cagatgttcg ttgagcggct ccagcccggc cacggggcgg tcttccacca
     2941 ggacgaagta ggtcgggccg ggcacgttga cgcgcaggcg caccagcacc aggtcgccaa
     3001 cgtgcacctc gcccagcggc ttgccagcag gcgtcaggaa ttggcggctg acagccagcg
     3061 ggccatcggc gtcgatctgc gcctggccga cggcgtagtc ctcgtgcagg gtgtagaaga
     3121 cgcggctgtc gccgctgacc gtcaggctgt gcgcgccggg cgtcagggcc tcggccggca
     3181 gggtcagcgt ggcgctcagc tcgccggcgg ccagcgtggt gttgatgggc ggcgcgccgt
     3241 cgagggccac gctggcgctc agggctgtct cgcccaggcc gctggcctcg atgtggtcgg
     3301 ccacggccag gatggtgtag gccgtctcgt tggtcgtgcc ccaacccagg ccgcggcgtt
     3361 gcgccatcag ccagcgcacg gccggcgctt ccagcgggtc gccggggcgc aggcggacga
     3421 aggcccgcag cgccagcgct gtcgagcgcg tggccgaggc catcgtcttg ctgcggtact
     3481 ggccgtcgtt gccgcccacg ccccagtggg cgcggccgtc ggcgacaatg gcctgcgccg
     3541 ccagccgggc catgactgcc tgcgccgcgt ctgcttcgcc ggcggcgtcg agggccaggg
     3601 ccagcgcggc caggctgaag gcgtcgaggt cgtcgtcggg cagggcggcc agttccagcg
     3661 ccgccgcgcc gttgggctgc ccggcgacgg ccaggctgta gagcatcatc gcccgcgtgc
     3721 ggttgtcggc cttgtcgagg ctgtcgttca ggtaggtcgc gccgcgcgcg atgacggccg
     3781 ggtcaatctc gtggcccgcc tcgaccatcg tcgccagccc gaagacgacc caggcggtct
     3841 ggtagggggt gctgtcgtcg tcgaaccacc agccccagcc gccgtcgtcg tgctggaagc
     3901 cgtagaggcg ttgggcgctc tcgttgacca gccgccccag ttccagcccc tccggctggg
     3961 gcgcgcccag ggcgtcgaag gcgcggtcaa cggcgacgtt gggcagggcg cggctcatgg
     4021 tctgctcgac gcagccgtag gggaagccgg tcaggtactc caggccgttg agcagcgagc
     4081 cggccggcga gcggctcaac tccagcgtca cggcgctgtg ctccggcagc gtgcccgccg
     4141 gcacggtgat ggtcagcgcc gtcgcgccct cgaagtcgcc
//
LOCUS       DAOSMQ010000276         4174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21388_length_4174_cov_4.865259, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000276 DAOSMQ010000000
VERSION     DAOSMQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21388_length_4174_cov_4.865259"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="PK829_09680"
     CDS             <1..1721
                     /locus_tag="PK829_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41526.1"
                     /translation="PAAAQDEPSNAFGVVFISSAEDRLPGSRRTADSLEQQYRNGLAT
                     GATWNRWPLYWFNIEPSPGVFNWAAQDATVQADIAHGLRLDAILLGTPAFYTTGNAAR
                     ASRVPRLRPGELSLDAIERATPAGLYEPTFADGTDTPAPGKAPNPANKWAVFTTAAVN
                     RYKPGGTLAQANGWPAGVGVTHWEMWNEPDLTIFWDASVEDYARLLKVGYLATKAADP
                     GATVLFAGLANDYRKLEYYRDVLTVLAADPAAATYGFYHDILATHSYFHAWKSWYHVY
                     RARGTMREFGFEKPIWLNESGVPAWDDYPGPVWDPASALRATTAEQAAYTIQSAMYAM
                     FTGATAVFHFQLYDGCGNQPAGTDFPPHNGELCDANGQYNGKPCAGDANGLYRNPPDM
                     LCFTQHDSPGSPRPNLAAFRVLTTYLAGVEPYWRVRAGEAEVCRMENPNQNPGIPGDE
                     FVLTQPQEIIAFYRPATQERVIGMWTLCGRNETAIVDATSLNGRATLVYADGHTQPIA
                     AVQGHYVIALPPATNRNPFPGGASPNLLYAIGGSPVLLIEKDERGASWPNHAYAPVVA
                     WQLAGP"
     assembly_gap    1877..1976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2234..4024)
                     /locus_tag="PK829_09685"
     CDS             complement(2234..4024)
                     /locus_tag="PK829_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU41527.1"
                     /translation="MADRQRDTIIDRLQQHRRTRPAAPAYYTRLGAAWVPTTWEEYVH
                     QVRTAARALMTLGVGAGDVVCMLGFNRPEWAIGQLAAMMIGGVGAGIYATNSPPEVQY
                     ILNHSAAPVVILENEGQWAKINAIRANLPHLRAAVLMQGTKIDDPLAMTWEAFLALAA
                     DSSEAALDTRIAAIEMEHLASLIYTSGTTGPPKAVMLSHKNLASTAYHGQQLFDLTPN
                     DVLLSYLPMSHIAEQMFSIHNGASVGYPVYYAESLTRMPDNLREVQPTIFFGVPGVWE
                     RFRNRVSERLDEAHGARKRIADWAQGVGKEVVALRNQGQEPTGALAAKYRLAERLVFS
                     KVKPLLGFSRVRVAVSGAAAIDKSVLEFFSGLDVIVFEVYGQSEGCGPTTFNRRGATR
                     FGTTGQAWPGSEVKLGPDGEVLMRGPNVFMGYYKDEAATRETLVDGWLHSGDLGRFDE
                     DGFLTIVGRKKDIIITSGGKNIAPRNVEAALRNLELVGDAVLVGEGRKYLCALLTLDP
                     EKVERFAEANGIVGAELHNHPKVIEAIQADIDENVNPQFARVEQIRKFTLLPRPFSIE
                     GGEMTPTLKLKRKAICDMHVSDIEAMYTEE"
BASE COUNT          661 a         1372 c         1334 g          707 t
ORIGIN      
        1 cgcccgccgc ggcccaagac gaaccgtcca acgccttcgg cgtcgtcttc atcagttcgg
       61 ccgaggatcg cctgcccggc agccggcgca cggccgactc gctggagcag cagtaccgga
      121 atggcctggc caccggggcg acgtggaacc gctggccgtt gtactggttc aatatcgagc
      181 caagccccgg cgtcttcaac tgggcggccc aggacgcgac cgtgcaggcc gacatcgccc
      241 acgggctgcg gctggacgcc atcctgctag gcacgccggc tttctacacc accggcaatg
      301 ccgcccgcgc cagccgcgtg ccacgcctgc ggccgggtga gctgagcctg gacgccatcg
      361 aacgggccac gcccgccggc ctttatgagc cgaccttcgc cgacggcacg gacacgcccg
      421 cgcccggcaa ggcccccaac ccggccaaca agtgggcggt cttcacgacg gcggccgtca
      481 accgctacaa gcccggcggc acgctggccc aggccaacgg ctggccggcc ggcgttggcg
      541 tcacccactg ggaaatgtgg aatgagccgg acctgactat cttctgggat gccagcgttg
      601 aggactatgc ccggctgctc aaggtcggct atctggctac caaggcggcc gaccccgggg
      661 cgacagtgct cttcgccggg ctggccaacg actaccgcaa gctggagtac taccgcgacg
      721 tgctgaccgt gctggctgcc gacccggccg cggccaccta cggcttctac cacgacattc
      781 tggccacgca cagctacttc catgcctgga aatcgtggta tcacgtctac cgggcgcggg
      841 ggacgatgcg tgagttcggc ttcgagaagc ccatctggct caacgagtcg ggcgtacccg
      901 cctgggacga ctatcccggg ccggtgtggg acccggccag cgccctgcgg gcgacgacgg
      961 ccgagcaggc ggcctacacc atccaatcgg ccatgtatgc catgtttacc ggggcgacgg
     1021 ccgtgttcca cttccaactc tacgacggtt gcggcaacca gccggcgggc acggacttcc
     1081 cgccccacaa tggcgagcta tgcgacgcga acggccagta caacggcaag ccctgcgccg
     1141 gcgacgccaa cggcctttat cgcaacccgc cggacatgct ctgcttcacg cagcacgact
     1201 cacccggctc gccgcggccg aacctggccg cttttcgcgt gctgacgact tacctggccg
     1261 gcgtggagcc gtactggcgc gtgcgcgccg gcgaggccga ggtctgccgc atggagaatc
     1321 ccaatcagaa ccctggcatt cccggcgatg agttcgtgtt gacgcaacct caggagatca
     1381 tcgcctttta caggccggcg acgcaagagc gcgttatcgg tatgtggacg ctctgtgggc
     1441 gcaatgagac ggccatcgtt gacgccacca gcctcaacgg ccgggcgaca ctggtctacg
     1501 ccgacggcca cacacagcct atcgccgctg ttcagggaca ttacgtgatt gccctgccac
     1561 cggcgaccaa ccgcaacccc tttcccggcg gcgcgtcgcc caatttgctc tatgccatcg
     1621 gcggctcgcc ggtgctgctc atcgagaagg acgagcgcgg cgcaagctgg cccaaccacg
     1681 cctacgcgcc ggtggttgcc tggcagctgg ctgggccata gccagcgtgc cgttcgcagg
     1741 aggcttccgt ggacaacccc attttgcgcg acttccccta tagcttcgag acggagcggc
     1801 tgatcattcg cgggccgctg ccggaagacg ccgcgccgct gcaccaggcc gtgctggagt
     1861 cgcagccgga gttagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcg
     1981 cgcggcgtgt tgagattcgc tgcgacgtga agaacacgcg tagcgccgcc gtggcccggc
     2041 gcgccggcta cgcgcttgaa ggcacattcc acagcgacgc ccgcgaccac atctccggcg
     2101 ggttgcgcga cacgctgatc tttgcgcagg ttcgggcgga ttaggggggc caggttccag
     2161 gggccaggtt ccagggaaaa cgctcttccc tggaacctag aacctggaac ctggaacctc
     2221 cctctgcgta ggtctattcc tcggtgtaca tggcctcaat gtcagacacg tgcatgtcgc
     2281 agatggcctt gcgcttgagc ttcagcgtcg gcgtcatctc gccgccctcg atgctgaagg
     2341 gccggggcag cagggtgaac ttgcggatct gctccacacg cgcgaactgc gggttgacgt
     2401 tctcgtcaat gtcggcctga atggcctcga tgactttggg gtggttgtgc aactccgcgc
     2461 cgacaatgcc gttggcctcg gcgaagcgct cgaccttctc cgggtcgagc gtcagcaggg
     2521 cgcagagata cttgcgcccc tcgcccacca gcaccgcgtc gcccaccagt tccaggttcc
     2581 tcagggccgc ctcgacgttg cgcggggcga tgttcttgcc gccggaggtg atgatgatgt
     2641 ccttcttgcg gccgacgatg gtcaggaagc cgtcctcgtc gaagcggccc agatcgccgg
     2701 agtggagcca gccatcgacc agcgtctcgc gcgtagccgc ctcgtccttg tagtagccca
     2761 tgaagacgtt gggcccgcgc atgaggactt cgccgtcagg gccgagcttg acctcgctgc
     2821 ccggccaggc ctggccggtc gtgccgaacc tggtcgctcc gcggcggttg aacgtcgtcg
     2881 gcccacagcc ttccgactgg ccgtagacct cgaagacgat tacgtccagg ccgctgaaga
     2941 actccagcac gctcttatcg atggccgccg cgccggagac ggccacgcgc acccggctga
     3001 agcccagcag cggcttgacc ttgctgaaga ccagtcgctc ggccaggcgg tacttggctg
     3061 ccagcgcgcc ggtgggttcc tggccctggt tgcgcaaggc cacgacctcc ttgccaactc
     3121 cctgtgccca atcggcaatc cgcttgcgcg cgccgtgggc ctcgtccagt cgttcagaca
     3181 cgcggttgcg gaagcgttcc cagacaccgg gtacgccgaa gaagatcgtc ggttgcactt
     3241 cgcggaggtt gtccggcatg cgggttagcg actcggcata gtagaccgga tagccgaccg
     3301 atgcgccgtt gtggatggag aacatctgct cagcgatatg ggacatgggc aggtaggaca
     3361 gcagcacgtc gttgggcgtc aggtcgaaga gttgctggcc gtggtaagcc gtggaggcca
     3421 gattcttatg cgatagcatg accgccttgg gcgggccagt cgtgccagag gtgtagatga
     3481 ggctggccag gtgctccatc tcaatggcgg cgatgcgggt atcgagggcg gcttcgctgc
     3541 tgtcggccgc cagagcaagg aatgcttccc aggtcatcgc caacgggtcg tcgatcttcg
     3601 tgccctgcat gagcacggcg gcgcgcaagt ggggcaggtt ggcgcggatg gcgttgatct
     3661 tggcccactg gccttcgttc tccagaatga cgaccggcgc ggccgagtgg ttgaggatgt
     3721 actggacttc gggtgggctg ttggtggcat agatgcccgc gccgacgccg ccgatcatca
     3781 tggccgccaa ctggccgata gcccactccg gccgattgaa gccgagcatg cagaccacgt
     3841 cgcccgcgcc gacgccgagg gtcatcagcg cccgggcggc ggtgcgcacc tggtggacgt
     3901 actcctccca cgtggttggc acccatgccg cgccgagccg ggtatagtag gcaggcgcgg
     3961 ccggccgggt gcggcggtgt tgctgcaaac ggtcgataat ggtgtcacgt tgcctatctg
     4021 ccatcggtca gtttcccccc ttactggctg ctgttttaag gtcacgcgcc gtctctgtca
     4081 gggcgagcgc cgggattcta cctgatcatg ccgtgggttg tcgaactgta gtcggtcttc
     4141 gcgcccccgc ccgtagcaca gcctgtcagg ctgt
//
LOCUS       DAOSMQ010000277         4171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21408_length_4171_cov_4.746356, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000277 DAOSMQ010000000
VERSION     DAOSMQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21408_length_4171_cov_4.746356"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PK829_09690"
     CDS             complement(<1..288)
                     /locus_tag="PK829_09690"
                     /inference="COORDINATES: protein motif:HMM:NF039193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HOU41528.1"
                     /translation="MSNEELQGYCLKCKEKRVIQNPVAEWAANGSPATRGTCPVCGGT
                     IYLRGRTPAHDTLPKPAATATRKATTAAKSKKTPAAGKSGRTTVAGKGQKAS"
     gene            complement(326..1411)
                     /locus_tag="PK829_09695"
     CDS             complement(326..1411)
                     /locus_tag="PK829_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU41529.1"
                     /translation="MRIVIFAGGAGRRLWPISRESSPKQFEPIVGEESTVQMAVDRVA
                     PAYGFDSITIATNERYVDIVRRQLPRIAAGNILIEPARRDLGPAVGLAMAHLGRHEAD
                     TPVAILWSDNHIKNVPAFIGLLGAAEGLVAAKTADIVFIGETPRFANENLGWIGLGEM
                     AGRVGALPYYRFDSLTYRPPLERCQEIFAGGRHVWNTGFFVTTPAYVREQYRRHSPQM
                     WAQLATIEAAIGSESYDDTLRRVYPEMKAISFDDAILTHVDAERALVLHGEMGWSDPG
                     TLYALKEALNPDPEANVTKGLVIDAASKDSLLYNYEPGKLVVAVGLEGMIVVNTDDAI
                     LIVHKNNIPLVKRVVNGLQGTELESYS"
     gene            complement(1421..2545)
                     /gene="dprA"
                     /locus_tag="PK829_09700"
     CDS             complement(1421..2545)
                     /gene="dprA"
                     /locus_tag="PK829_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU41530.1"
                     /translation="MSDLKYWLAFNYVNGIGPAKVQALLGAFGDLERAWQAGEGQLRD
                     IGFDARAIDSLREARQKLDLDAYAAKVEASGARTLTWESADYPALLRQIPAAPPVLFV
                     RGAFAPVDDWAVAIVGTRRLSAYGRLVTHDLATALARNGITIVSGLARGIDGVAHRAA
                     LEADGRTIAVMGCGIDRVYPPEHRDLAHAIVDGHGALVTDFPLGTEPSSVNFPARNRL
                     ISGLSLGVLVIEAGERSGALITARFALEQDRDVFAVPGNINSPVSVGTNRLIQQGAKL
                     VTRIEDILEELNLRMVTEQQAAQAVLPDSAEEAALLSQLSSQPLHVDDLGRLTGMPSY
                     LVSSTLTLMELKGMVQQVGAMNYVRLREPDVAYQADTPEE"
     gene            complement(2610..3188)
                     /gene="rimM"
                     /locus_tag="PK829_09705"
     CDS             complement(2610..3188)
                     /gene="rimM"
                     /locus_tag="PK829_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOU41531.1"
                     /translation="MSDARPDEAHEQPEGGSADSSAEPRFLAVGRVIKPHGVHGEVRV
                     EPMTARLERFGWLKDIYLGERAPRRVGVVSVRYHQGLVLLKLDGYTTRTEAEALRNEL
                     LQVPEEEAIPLEEGEYFLHQLLGLEVVTEDERSLGRLTEVLETGANNVFVIDGPGGQH
                     LVPDIPDVVLAVDVAAGRIVIRPLPGLLDNKA"
     gene            complement(3199..3444)
                     /locus_tag="PK829_09710"
     CDS             complement(3199..3444)
                     /locus_tag="PK829_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOU41532.1"
                     /translation="MKDLVTFIVESLVDNPDEIQVTEVRGRGATVVELAVAQPDMGRI
                     IGKSGRVIDSIRTLLQVRAAKDGKRVELELLEADELR"
     gene            complement(3449..3910)
                     /gene="rpsP"
                     /locus_tag="PK829_09715"
     CDS             complement(3449..3910)
                     /gene="rpsP"
                     /locus_tag="PK829_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOU41533.1"
                     /translation="MSRTGKKRQPSYRVVVAEKEAKRDGRVVERLGHYNPLTNPAEFR
                     IDEARALYWLSVGAQPSDAVRVLLNKQGTFDRLARLHAGESLDTLVAEYRGVEAPAPV
                     AEVEQEAAPSLVERVEAAASSVVEAVAAPVAAVVDRIGDAVAAVLGSDEEE"
     gene            complement(4019..>4171)
                     /locus_tag="PK829_09720"
     CDS             complement(4019..>4171)
                     /locus_tag="PK829_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU41534.1"
                     /translation="RKRRIAAGSGTTVQDINILLKQHREMQEMMKQFKGGRGRSALSQ
                     LLGGKM"
BASE COUNT          691 a         1391 c         1317 g          772 t
ORIGIN      
        1 cgaagccttt tggcctttgc ccgcgaccgt tgtccggcca ctcttgccgg ccgccggggt
       61 cttcttactc ttggccgcgg tcgttgcctt gcgtgtcgct gtcgctgccg gcttcggcag
      121 ggtatcgtgg gcgggtgtgc ggccgcgcag gtaaattgtg ccgccacaga cggggcacgt
      181 gccgcgtgtc gccggcgagc cgttggccgc ccactccgcc acggggttct gtatcacgcg
      241 cttctcttta cacttcaggc agtagccctg tagttcttcg ttgctcatgc tctgctttac
      301 agtctcgcct gtggcgagac tggcctcagc tataggattc cagctcggtc ccctgcaagc
      361 cattgacaac ccgtttgacc agggggatgt tgttcttatg gacaatcaga atggcatcat
      421 ctgtattgac cacgatcatt ccctctaacc cgacagccac gacgagcttg cccggctcat
      481 agttatagag caggctatct ttggacgcgg cgtcgatgac caatcctttc gtcacattgg
      541 cctcagggtc ggggttcagc gcctctttca gcgcgtacag cgtgcccggg tcgctccagc
      601 ccatttcacc atgcagtacc agggcgcgct cggcgtccac gtgggtcagg atggcgtcgt
      661 caaagctgat cgccttcatt tccgggtaga cgcggcgcaa ggtgtcgtcg taactctcag
      721 agccaatggc cgcctcgatg gtggccaact gtgcccacat ctgcggtgag tggcggcgat
      781 actgctcgcg cacataggcc ggcgtcgtca cgaagaaacc cgtgttccag acgtgccggc
      841 ccccggcgaa aatctcctgg cagcgctcta gcggcggccg gtaggtcagc gagtcgaaac
      901 ggtaataggg cagcgcgccc acccgcccgg ccatctcccc caggccgatc cagcccagat
      961 tctcattggc aaagcgcggc gtctcgccga tgaaaacaat gtcggccgtc ttggcggcga
     1021 ccaatccctc agcggcccct aacagaccaa tgaaggcggg cacattctta atgtggttat
     1081 cgctccagag gatggccacc ggcgtgtcgg cttcgtgccg gcccaggtgg gccatggcca
     1141 gccccacggc cggccccaga tcgcgccgcg ccggctcgat gaggatattg cccgccgcaa
     1201 tccgcggcag ctgccggcga acgatgtcga cgtagcgctc gttggtggcg atggtgatgc
     1261 tatcgaagcc gtaggccggt gcgacgcggt cgacggccat ctgcacggtc gattcctctc
     1321 ccacgatcgg ctcaaactgc ttcggtgaac tctcgcgact gataggccat aggcgacggc
     1381 cggcccctcc ggcgaagatc acaatacgca tcgtctcccc ttactcctcc ggcgtgtcgg
     1441 cctgataggc gacgtccggc tcgcgcaggc gcacgtagtt catagcgcct acctgttgca
     1501 ccataccctt cagttccatc aaagtgagcg tgctgctgac gaggtagctg ggcatgccgg
     1561 tcaggcggcc gagatcatcg acgtggagcg gctgcgacga caattgcgac agcagcgcag
     1621 cctcctcggc cgaatcgggc aacacggcct gagccgcctg ttgctccgtc accatacgca
     1681 ggttcaactc ttcgaggatg tcctcgatgc gcgtcaccag cttggccccc tgctggatga
     1741 gccggttcgt gcccacgctg accgggctat tgatgttacc gggcacggca aacacgtcgc
     1801 ggtcctgctc cagggcgaag cgcgccgtga tcagcgcgcc actgcgctcg cccgcttcga
     1861 tgaccagcac gcccagcgac agaccgctga tgagccggtt gcgggccggg aagttgacgc
     1921 tgcttggctc tgtgcccagc ggaaaatcgg tcacgagcgc cccgtggcca tcgacaatgg
     1981 catgggccag gtcacggtgt tccggcgggt agacgcggtc gatgccacag cccatgacgg
     2041 caatggtgcg gccgtcggcc tccagcgcgg cgcggtgggc gacaccgtcg atgccgcggg
     2101 ccagcccgct gacgatggtg atgccgttgc gggccagggc ggtagccaga tcgtgggtca
     2161 ccaggcggcc gtaggccgac agtcgccgcg tgccgacgat agccactgcc caatcatcca
     2221 ccggcgcaaa cgcgccgcgg acgaagagca ccggcggcgc ggccggaatc tggcgcagca
     2281 gcgccgggta gtcggcgctt tcccaggtca gcgtccgcgc gccactggcc tccaccttgg
     2341 ccgcataggc gtcgaggtcg agcttctgcc gcgcctcgcg caggctgtcg atggcgcgcg
     2401 cgtcgaagcc gatgtcacgt agctggccct cgccagcctg ccaggcccgc tccaggtcgc
     2461 caaacgcgcc cagcagcgcc tggactttgg ccgggccaat gccgttgacg tagttgaagg
     2521 cgagccagta tttgagatcg gacatgggca gaggatacct ctagctgccg ggcgcgcaag
     2581 aggagtgcgg acggcagtgg cgaagcgaac taggccttgt tgtcaagaag tcccggcagg
     2641 gggcggatga cgatgcggcc ggcggcgaca tcgaccgcga gcacgacgtc gggaatgtcg
     2701 ggcacgagat gctggccgcc cggaccgtca atcacaaaga cgttgttggc cccggtctcc
     2761 agcacttcgg tcagacgccc cagggatcgc tcgtcctcgg tgacgacttc caggccgagc
     2821 agttggtgaa ggaagtattc gccctcttcc aggggaatgg cttcctcctc aggaacctgc
     2881 aacaactcgt tgcgcagggc ttcggcctcg gtgcgcgtcg tgtagccgtc cagcttcagc
     2941 aggacaaggc cctggtgata gcgcacgcta accacgccga cgcggcgcgg ggcgcgctcg
     3001 ccgaggtaga tgtccttcaa ccagccgaaa cgctccagcc gggcggtcat tggctcgacg
     3061 cgcacttcgc catgcacgcc gtggggcttg atgacgcggc cgacggccag aaaacgaggc
     3121 tcggcggaac tgtccgcgga gcctccctct ggttgttcgt gtgcctcgtc tggtcgcgcg
     3181 tcgctcaagt gaccgtcctc agcgaagctc atcggcctct agcagttcca actcgacacg
     3241 cttgccgtcc ttggccgcgc gcacttgcag cagcgtgcga atcgagtcga taacccggcc
     3301 gctcttgccg atgatacggc ccatgtcggg ctgggccacg gccagctcca cgactgtcgc
     3361 cccgcggccg cgcacctcgg tcacctgaat ctcgtccggg ttgtcgacga gcgactcgac
     3421 gatgaaggta accaggtctt tcatgagctt actcttcctc gtcgctgccc aggacggcgg
     3481 cgacggcatc accgatgcgg tcaaccacgg ccgccacggg cgcggccacg gcctcaacga
     3541 ccgagctggc ggcggcttcg acgcgctcga cgagcgacgg cgcggcttcc tgctcgacct
     3601 cggcgaccgg cgcgggcgct tcgacgccgc ggtactcggc caccagcgtg tccagcgact
     3661 cgccggcgtg caggcgggcc aggcggtcga acgtgccctg cttgttgagc agcacgcgca
     3721 cggcatcgga cggctgcgcg ccgacgctca gccaatagag cgcgcgcgcc tcgtcgatgc
     3781 ggaactccgc cgggttggtc agcgggttat agtggccaag gcgctcgacg acgcggccgt
     3841 cgcgcttggc ctctttctcg gcgacgacaa cgcgatagct cggctgccgc ttcttcccgg
     3901 ttcgactcaa acgaattcgt agcatggtct aatctgtcac tccaaactat gtaattacga
     3961 attaggaatt cagagtgtct cttacttctt caattcctaa ttcctcattc ttaattgatt
     4021 acatcttgcc gcccaaaagc tggctgaggg cgctgcgccc gcgaccgccc ttgaactgct
     4081 tcatcatctc ctgcatctcg cggtgctgct tgagcaggat gttaatgtcc tgaaccgtcg
     4141 tgcccgaccc ggcagcgatg cgccgcttgc g
//
LOCUS       DAOSMQ010000278         4157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21493_length_4157_cov_4.940761, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000278 DAOSMQ010000000
VERSION     DAOSMQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21493_length_4157_cov_4.940761"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..533
                     /locus_tag="PK829_09725"
     CDS             153..533
                     /locus_tag="PK829_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU41535.1"
                     /translation="MTEPVAPRQRLGSWGEGVAAVRLEAEGYTIVARNWRCAAGEIDL
                     VARDGETLVFVEVKTRRGRAYGAPEEALTPRKAEKLLQLGAQYVYEQALGDVNWRIDL
                     VAIELDARGLLLRCEHVRNAVLGW"
     gene            555..1742
                     /locus_tag="PK829_09730"
     CDS             555..1742
                     /locus_tag="PK829_09730"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU41536.1"
                     /translation="MSDRAALPADRTILVLTNDSGLGHRQAALAVVAALERRYGAHGR
                     PVLVNPLEEPGAPALLRDAQSDYNRIVRLAPWLYRFGHGLANTYAPTRLMERLEVLTL
                     AETMANVLAREQPDVVVTTHPAFVYLLAHHRRRHGQTWPLVVLVTDLARLQRLWFRPE
                     VDLYLAPTDEAAALAIRRGIAAERVRVTGIPVDLRLSQPIAAAETRRANGWDATRPTV
                     LAVGSRRVQEFPAFLRALNDADLPIQLIAVTGDDDELLDEVRAIPWRLPAHLYGYVSD
                     LPNWLPAVDAVVTKAGGLTVAEALAAGRPLFIIQCTPMHERGNADYVVDRGAGVLAGA
                     PAALVSALRQALADDGRLLAQMRDRARALGRPRAAFDAAELIWAQAERNLAPPDALLT
                     HER"
     gene            1732..2655
                     /gene="miaA"
                     /locus_tag="PK829_09735"
     CDS             1732..2655
                     /gene="miaA"
                     /locus_tag="PK829_09735"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU41537.1"
                     /translation="MSVEPRRPLLVLAGPTAVGKTAVSLALASRFDGEIVSADSRLFY
                     QGMDIGTAKPSPAERALVPHHLIDLCAPDETIGLGQFQRLAYAAIDAVQARGRLPILT
                     GGTGQYVMAVVEGWGVPAVAPRPALRAALEGLGGAEAGRWLAALDPAAAARIDPRNVR
                     RVVRALEVMLVSGRRMSDLQRKTPPPYDIYLLGLRRDRADLYARIDARVEAMMAAGLL
                     DEVRRLRDAGYANEPPLSGLGYQQLLAHLRGELSLGEAVARTQFETHRFARQQATWFR
                     ADDPRIAWFEADDRAAIVAAVSDWLFAHADR"
     gene            2780..3373
                     /locus_tag="PK829_09740"
     CDS             2780..3373
                     /locus_tag="PK829_09740"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOU41538.1"
                     /translation="MPSLFTLPLYRLTFIIVLAIWATPDVFDSIRRRPRGNNVVFHER
                     GSHWLFRIGLTLALATAIWLALARPEGTLPYHRPYVFIAGVVVALAGLVLRRAAIRTL
                     GRFFTVDVATYTDQRVVDAPPFRRIRHPAYSGSMLSLLGTGLMLGHWLGLLLIALASI
                     VTFGYRVRLEERALLDALGEPYAAYMRRTRRFVPFLF"
     gene            3430..4059
                     /locus_tag="PK829_09745"
     CDS             3430..4059
                     /locus_tag="PK829_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41539.1"
                     /translation="MTDDTSLTPPEPEMIAAAMAAAQVAHDLTRGHDALRTAERRASL
                     ADLVSAAGVNDLMQGADMLAASEDLGTMSAVVHSLNEEDLQGVIEIGAISGELAVLSD
                     VLAAMELATMAGFLQGRSQRLRQLAVENILRYSAMRALSESMGETGETVGALGEEEVA
                     EGEARLDLAEAAAANSQAMAEAGRGMIEDGLALLTGDEELLGEGEDEAE"
BASE COUNT          578 a         1491 c         1437 g          651 t
ORIGIN      
        1 tgaggacgcc cacggccgct ggtcgcgccg gacggagaag tggtcggcca tgctagatca
       61 gttgacccaa ctcgtgcggc cgtcggacga cgccggcggc gacccgccgc gctggtggcc
      121 ggccaacgcc ctgacggcgc gcagggagcg ccatgactga gccggtcgcg ccgcgccagc
      181 ggctgggcag ttggggcgag ggcgtcgccg ccgtgcggct ggaggccgag ggctatacca
      241 ttgtggcccg caactggcgc tgcgccgcgg gggagatcga cctcgtcgcc cgcgatggcg
      301 agacgctggt gtttgtggag gtgaagacgc gccgcggccg ggcctatggc gcgccagaag
      361 aggcgctgac accgcgcaaa gccgagaagc tgctgcaact gggggcacag tacgtctacg
      421 agcaggccct cggcgacgtc aactggcgca tcgacctggt ggccatcgag ttggacgcgc
      481 gcgggctgct gttgcgctgc gaacacgttc gtaacgccgt tttgggctgg tgagcgccga
      541 atttccaacc tcctatgagc gaccgcgccg cgctgcccgc tgaccgcacc attctcgtcc
      601 tgaccaacga ctccgggctg ggccaccgtc aggcggcgct ggccgtcgtc gccgcgttgg
      661 agcggcgcta cggcgcgcac ggccgtcccg tgctggtcaa cccgctggag gagccgggcg
      721 cgccggcgct gttgcgcgat gcccagtccg actacaaccg catcgttcgc ctggcgccct
      781 ggctctaccg cttcggccac ggcctggcta atacctacgc gcccacgcgg ctgatggagc
      841 ggttggaggt gctaacgctg gccgagacga tggccaacgt gctggcgcgg gagcagcccg
      901 atgtcgttgt caccacgcac cccgcctttg tttatctgct ggcccaccac cgccgccgcc
      961 acgggcagac gtggccgctg gtggtgctgg tgacagacct ggcccgcttg cagcggctgt
     1021 ggttccggcc ggaggtcgat ctctacctgg cgccgacgga cgaggctgcg gcgctggcca
     1081 tccggcgcgg catcgccgcc gagcgcgtcc gggtgaccgg catccccgtc gatctgcgcc
     1141 tcagccagcc catcgccgcg gccgaaacgc gacgagccaa cggctgggac gcgacacggc
     1201 cgaccgtgct ggccgtcggt agccggcggg tgcaggagtt ccccgccttc ctgcgcgccc
     1261 tcaacgacgc cgatctgccg atccagttaa tcgccgtcac tggcgacgac gacgaactgc
     1321 tggacgaggt gcgggcgatt ccctggcggc tgccggcaca cctgtacggc tacgtctcgg
     1381 acttgccgaa ttggctgccg gccgtcgatg cggtagtcac caaggccggc gggctgaccg
     1441 tggccgaggc gctggccgcc ggccggccgc tgttcatcat ccagtgtacg ccgatgcacg
     1501 agcggggcaa cgccgactac gtcgtcgatc gcggcgcggg cgtgctcgcc ggcgcgccgg
     1561 cggcgctggt gagcgctctg cggcaggcgc tggccgacga cggccgcctc ctggcgcaga
     1621 tgcgcgaccg cgcccgcgcg ctgggccggc cgcgcgccgc ttttgacgcc gctgaactga
     1681 tctgggcgca ggccgagcgc aatctggccc cgcccgacgc cctgctgacc catgagcgtt
     1741 gagccgcgcc ggccgctgct ggttctcgcc ggcccgacag ccgtgggcaa gacggctgtg
     1801 tcgctggcgc tggccagccg cttcgacggc gagatcgtct cggccgactc gcgcctgttc
     1861 taccagggca tggacatcgg cacggccaag cccagcccgg ccgagcgcgc cctcgtgccc
     1921 caccatctca tcgacctgtg cgccccggac gaaaccatag gcctggggca gtttcagcgg
     1981 ctggcctacg cggccatcga cgctgtgcag gcgcgcggcc ggttgcccat cctcaccggc
     2041 ggcacggggc aatacgtcat ggccgtcgtc gagggctggg gcgtgccggc cgtggcgccg
     2101 cggccggcgc tgcgggcggc gctggaaggg ctgggcggcg cggaggccgg gcgttggctg
     2161 gccgcgctcg atcccgccgc cgcggcgcgc atcgacccgc gcaacgtccg ccgcgtcgtc
     2221 cgggcgctgg aggtaatgct ggtcagcggc cggcgcatgt cggacttgca gcgcaagacg
     2281 ccgccgccct acgacatcta cctgctgggg ctgcggcgcg accgggccga tctctacgcg
     2341 cgcatcgacg cccgcgtgga ggcgatgatg gccgccgggc tgctggacga ggtgcgccgc
     2401 ctgcgcgacg ctggttatgc caacgagccg ccgctgtcgg ggctgggcta ccaacagcta
     2461 ctggcccacc tgcgcggcga gctgtcgctg ggcgaggccg tggcccgcac ccagttcgaa
     2521 acccaccgct tcgcccgcca gcaagcgacc tggttccgcg ccgacgaccc gcgcatcgcc
     2581 tggttcgaag ccgatgaccg cgcggcgatt gtggcggccg tgtctgattg gctctttgcc
     2641 cacgccgacc gctgacgaca gaccgccgac cgccgaccgc cgacgactga ccactggata
     2701 ctgacccact gcttactgac ccactgctta ctgacccact gcttactgac ttactgatta
     2761 ctgccccact gcccacccca tgccctccct cttcaccctc cccctctacc ggctgacctt
     2821 catcatcgtt ctggccatct gggccacgcc tgatgtgttc gactcgatcc gccgccgtcc
     2881 acgaggcaac aatgtcgttt ttcacgagcg cggctcacac tggctctttc gcattgggct
     2941 aacgctggcc ctggccactg ccatctggct ggcgctcgcc cggcccgaag gcacgctgcc
     3001 ctaccaccgg ccctatgtgt tcattgccgg cgtcgtcgtc gctctcgccg gacttgtcct
     3061 gcggcgggcg gccatccgca ccctcggccg cttcttcacc gtcgacgtcg ccacctacac
     3121 cgaccagcgc gtggtggacg cgcccccctt ccgccgcatc cgccacccgg cctacagcgg
     3181 cagcatgctc tccctgctgg gcacggggct gatgctgggc cactggctgg ggctgctgct
     3241 catcgctctg gcaagcatcg tgacgttcgg ctaccgcgtg cgccttgaag agcgggcgct
     3301 cctggacgcg ctcggtgaac cgtatgccgc ctacatgcgt cgcaccaggc gctttgtgcc
     3361 tttcctcttc tgacgggcgg gctataatgc cgttcgtctg aaccgtcaca gccgacagga
     3421 gaactcatca tgaccgacga cacttcgctc accccacccg aaccggaaat gatcgccgcg
     3481 gcgatggccg ccgcgcaagt ggcccacgac ctgacccgcg gccacgacgc gctgcgcacg
     3541 gccgagcggc gggcgtcgtt ggccgacctc gtctcggccg ccggcgtcaa tgacctgatg
     3601 caaggggccg acatgctggc cgcgtcggaa gacctcggca ccatgagcgc cgtcgtccac
     3661 tcgctcaacg aggaggattt gcagggcgtc atcgagatcg gcgcgatctc cggcgagttg
     3721 gccgtgctga gcgacgtgct ggcggccatg gaactggcca cgatggccgg cttcctgcag
     3781 gggcgcagcc agcgcctgcg gcagctggcc gtcgagaaca tcctgcgcta cagcgccatg
     3841 cgtgccctca gcgagtcgat gggcgagacg ggcgagacgg tcggcgcgct gggcgaggag
     3901 gaagtggccg agggcgaggc gcggctggat ttggccgagg ccgccgcggc caacagccag
     3961 gccatggccg aggccggccg agggatgatc gaggatggct tggccttgct gaccggcgac
     4021 gaagaattgc tgggcgaggg cgaggacgag gccgagtaac tcgccgggtc ggggcacagt
     4081 gccgcgtctt aatgtgcccc ttagaaagtg gactatagtc cgcctaattg gcgtttcccc
     4141 cttgacaggg tgtggct
//
LOCUS       DAOSMQ010000279         4156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21506_length_4156_cov_4.194587, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000279 DAOSMQ010000000
VERSION     DAOSMQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21506_length_4156_cov_4.194587"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..1064)
                     /locus_tag="PK829_09750"
     CDS             complement(267..1064)
                     /locus_tag="PK829_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOU41540.1"
                     /translation="MPAPQALLDKIAQFHDDRRRLLPTYNETEVRVHYINPLFEALGW
                     DMTNAHGRNEVKHEDRVDVRERDRLRAKAPDYGFYLDGRRRFFVEAKKPAVHLERDPA
                     PAHQLRRYAWTAKLPLSILTDFEELAVYDTRVRLRPDDSAAVARLRFWRYDDYAAVWD
                     EIAALFGRDAVLAGSLERFAEAEGGRKGIATVDAEFLRQLDEWRDVLARAVANRNRLS
                     QRDLNFAVQQLLDRVIFLRIAEDRGIEPYGQLQDLTQGAGHSSSTAC"
     gene            complement(1189..1677)
                     /locus_tag="PK829_09755"
     CDS             complement(1189..1677)
                     /locus_tag="PK829_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU41541.1"
                     /translation="MPVKSGTLSRSRPAGSGFPDCAALGLGRYLRAARLNAGWSYEAL
                     AGQAGLDRVTVIALESGLLPPADIRAEWLDRLAAALNEDAGDLRLLLNLPVARPEPAT
                     RAAAAAESRLLRLTVDDADDDHPAGSEAHPPYHGAGWIDCRARYRSIRPQRDPERLYF
                     DK"
     gene            complement(1895..2521)
                     /locus_tag="PK829_09760"
     CDS             complement(1895..2521)
                     /locus_tag="PK829_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU41542.1"
                     /translation="MARAAVDELMTYCRQSISAYVNSRGLDSDYCEDVIDDTCLYVST
                     HISGFAFRGRPLSHWFGAIAALKVKEQRRIARRNRREIAASELPGADGDWDDHPFDLP
                     QNLAHAHRSTQRYDSSDRNDPNLTAVLNNLTRQEADRLIHMALKSLPTDQRRIMMLIY
                     FNEITTAEEVGAILNMDPGTVRVYHLRARRRLEAVAVLKAIFDDLCFS"
     gene            complement(2751..2939)
                     /locus_tag="PK829_09765"
     CDS             complement(2751..2939)
                     /locus_tag="PK829_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41543.1"
                     /translation="MNMNILLEWTVQVNRLAAIGLQNSREVPVVEMTIQALVIVALVF
                     MFLGFYAGIRVARPRYWD"
     gene            3142..3336
                     /locus_tag="PK829_09770"
     CDS             3142..3336
                     /locus_tag="PK829_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41544.1"
                     /translation="MLSNPLEILWHSRKFWLAVVAVVQTAVFTLVPGFPDALWQAINV
                     ILLFLIGAIAAEDVALKWRR"
     gene            3378..>4156
                     /locus_tag="PK829_09775"
     CDS             3378..>4156
                     /locus_tag="PK829_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41545.1"
                     /translation="MSWLDRWRQRQQLAAAADLREGVGGWSSVVGAGGPFGRGDRPPD
                     DATRQYNDALLAWRTNPYAKRIIDIITDYTVGDGLTPVAPGELGRFIDTFWTHPQNRL
                     DLRLPELMDELSRAGDLFLVLFRNPADGMSYVRAVPKSEVVEIAAAENDWEREVAYVV
                     RASLAVGEQGGRGAGENSPLQPCPPAPLPANTTVFLSPAHPDAAAADAVMLHYTINRP
                     VGALLGESELASILPWLRNYSRMLEDRVRLNWAARAFLWFVT"
BASE COUNT          692 a         1341 c         1373 g          750 t
ORIGIN      
        1 atgcttgttc tcccgtctca aggatatagc cttcttcgcg actctacttt tccgggggaa
       61 ctccaccgtg gctgcggctg gcgacgacag cctaacaggc tgtactactg tctcctcgac
      121 accagcctgt agcacagcct gttaggctgt ggctgcggct gcggctggcg acgacagcct
      181 aacaggctgt actactgtct cctcgacgcc tagcctgtag cacagcctgt taggctgtgg
      241 ctgcggctgc ggctggcgac gacagcctaa caggctgtac tactggagtg gcccgcgccc
      301 tgggtcaggt cttgcagctg gccataaggt tcgatgccgc ggtcttcggc gatgcgcagg
      361 aagatgaccc ggtcgaggag ttgctggacg gcgaagttga ggtcgcgctg gctgaggcgg
      421 ttgcggttgg ccacggcgcg ggccagcacg tcgcgccact cgtccagctg gcgcaggaac
      481 tcggcgtcga cggtggcgat tcccttgcgg ccgccctcgg cctcggcaaa gcgctccagc
      541 gaaccggcca gcacggcgtc gcggccgaag agcgcggcga tttcgtccca gacggcggcg
      601 tagtcgtcgt agcgccagaa gcgcagccgg gcgacggcgg cgctgtcgtc gggccgcagg
      661 cgcacgcggg tgtcatagac ggccagttcc tcgaagtcgg tcaggatgct gaggggcagt
      721 ttggccgtcc aggcatagcg gcgcagctgg tgggcggggg cggggtcgcg ctccaggtgg
      781 acggccggct tcttggcctc gacgaagaag cggcggcggc cgtcgaggta gaagccgtag
      841 tcgggggcct tggcccgcag ccggtcgcgc tcgcggacgt cgacgcggtc ttcgtgctta
      901 acctcgttgc ggccgtgggc gttggtcatg tcccagccga gggcctcgaa gagggggttg
      961 atgtagtgga cgcggacttc ggtttcgttg taggtcggca ggagccgccg gcggtcgtcg
     1021 tggaactggg cgatcttgtc gaggagggct tgtggcgcgg gcatgggcag aggttaacgc
     1081 aaagacgcca agacgcaagg aggggggagg ggggcgagcc acagtcgacg ccaagacgca
     1141 gggtgacggg actcttttca cgccctggcg tcctggcgtc agactgttct acttgtcgaa
     1201 gtaaaggcgt tccgggtcgc gctgggggcg gatggagcga tagcgggcgc gacagtcgat
     1261 ccagcccgcg ccgtggtaag gtgggtgggc ctccgacccg gcggggtggt catcatcggc
     1321 gtcatcgacc gtcaggcgca gcagacggga ctcagccgcg gccgcggccc gggtcgccgg
     1381 ctcgggtctg gccacgggta ggttcagcag caggcgcagg tcgccggcgt cctcattcag
     1441 agccgcggcc agccggtcga gccattcggc gcggatgtct gccggcggca acaggccgct
     1501 ctccagggcg atgaccgtca cgcggtcaag tccggcctgt ccggccaggg cttcgtagga
     1561 ccaaccggca ttgagccggg cggcgcgcag atagcggccc agacccagcg ccgcgcagtc
     1621 cggaaaaccg ctgcccgctg gccggcttct ggaaagcgta ccgctcttta ccggcactac
     1681 ccttgaactg ttgctccggc caatcagtcc ctctccagat gtacgctttg tgagcatgtc
     1741 ttctccacca tcctgtaaat aaagtgccat tgagcggccg cctggccggt cgccacgaat
     1801 aaagatgggc tgtcgcctgg cccagaaccg gcgttccaaa aaatgaaaag ccgcgccgga
     1861 gagcgtgcca agtacactcc tgggcgcggc caggtcagga aaaacaaaga tcatcgaaga
     1921 tcgctttcaa cacagccact gcttccagcc gccggcgggc gcgcagatga tagacgcgca
     1981 ccgtgccggg gtccatgttc agaatcgcgc cgacctcctc ggccgtggtg atctcattga
     2041 agtagatcag catcataatc cggcgctggt cggtcggcaa ggatttcagc gccatgtgga
     2101 tcagccggtc ggcttcctgg cgggtcagat tgttcagcac cgcggtcagg ttggggtcgt
     2161 ttctgtcaga ggagtcatag cgttgggtcg aacggtgggc gtgggccagg ttctggggca
     2221 ggtcaaaggg atggtcgtcc cagtcgccgt ccgcgccggg caactctgag gcggcgatct
     2281 cgcgccggtt gcgccgggcg atgcgccgtt gttccttgac cttcagagcg gcgatagccc
     2341 cgaaccagtg gctgaggggc cgtccgcgga aagcgaagcc gctgatatgc gtgctcacgt
     2401 ataggcacgt gtcatcgatg acatcttcac agtagtcgct atccagcccg cggctgttca
     2461 cgtatgcgct aatgctctgg cgacaatagg tcatcagttc atcgacggct gccctggcca
     2521 tttctggcga cccctgttgg attctccgga gtaagtctcg gtcatatgcc aactcgcccg
     2581 ggccgcccct ttccggcgct gctggattgg ccatcccttc cgacatcctg cctgcctcct
     2641 tccctgcttg ccgaccgcga ctgtcgttag tatatcatgg cgtggggcgg ccggagtgtt
     2701 acagttgcct gccactccga ccgccgttca accactcctg gcatccagtg ttaatcccag
     2761 taccgcgggc gagccacgcg gataccggcg tagaatccca aaaacataaa caccagggcc
     2821 acaatgacga gggcctggat ggtcatctcg accaccggga cttcacgcga attttgcagg
     2881 ccgatcgcgg ccaggcgatt gacctgcacc gtccactcca gcaagatgtt catgttcatc
     2941 cccttttcga gcctcctagt gatcaatggc tgcccggggc caattcgtta ccggcaaata
     3001 gcccccagcg aggctactgg atcgatctgc attaccccca tgtcgaaaat cgaattgcgc
     3061 cagcactttt tagtttgcta gactgttcgc tagaacaaat gttcgcgccg ggcaaccggc
     3121 cagcgaccca aaggagacat catgttaagc aacccgctcg aaatcctgtg gcacagccgc
     3181 aagttctggc tggccgtcgt cgccgtcgtc cagacggccg ttttcaccct tgtgcccggc
     3241 ttccccgacg ccctgtggca ggccatcaac gtcatcctgc tgttcctcat cggcgcgatc
     3301 gccgccgagg acgtggccct gaaatggcgg cgctgagcgc cccgccgtct accgccgacc
     3361 acccccgagg agacaccatg agctggctcg atcgatggcg acaacgacaa caattggccg
     3421 cggcggccga cctgcgcgag ggcgtgggcg gctggtcatc cgtggtcggg gctggcgggc
     3481 cgtttggccg cggcgaccgc ccgcccgacg acgcgacccg gcagtacaac gatgcccttt
     3541 tggcctggcg caccaatccc tacgccaagc gcatcatcga cattatcacc gattacaccg
     3601 tcggcgacgg cctgaccccc gtcgcccccg gcgaactcgg ccgcttcatc gacaccttct
     3661 ggacgcaccc gcagaaccgg ctcgacctgc gtctgccgga actgatggac gaactgagcc
     3721 gcgccggcga cctgttcctc gtcctcttcc gcaacccggc cgacggcatg agctacgtcc
     3781 gcgccgtgcc caagtcggag gtggtcgaga tcgccgcggc cgaaaacgat tgggagcgcg
     3841 aggtggccta cgtggtgcgc gcgtccctgg cagtggggga gcaggggggc aggggcgcgg
     3901 gggagaattc gcccctgcag ccctgccccc ctgcgcccct gccggccaat acgaccgtct
     3961 tcctctcgcc ggcccacccc gacgccgccg cggccgacgc ggtgatgctc cactacacca
     4021 tcaaccggcc cgtcggggcg ctgctgggcg agtcggaact ggccagcatt ttgccctggc
     4081 tgcgcaatta cagccggatg ttggaagacc gcgtgcggct caactgggcg gcgcgggcct
     4141 tcctgtggtt tgtcac
//
LOCUS       DAOSMQ010000280         4139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21636_length_4139_cov_5.311949, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000280 DAOSMQ010000000
VERSION     DAOSMQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21636_length_4139_cov_5.311949"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /gene="galT"
                     /locus_tag="PK829_09780"
     CDS             <1..747
                     /gene="galT"
                     /locus_tag="PK829_09780"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOU41546.1"
                     /translation="LGNAAQRGGFHGHAGQALRDVVVQLARQPPALVLVGGDQPPAQR
                     MRLAFGALAAVYLGVREDGRWLWAAGALAGLAMFTRNDGLALIAAGALGGLWLAWRRG
                     AYPRLRHVLGAGVTLAIVLLPWVALAPFWAVWPYETLLLPRGHCGALTDLGDAARDAL
                     ADILGRLTRRYNRVFDVPFPYSMGFHQQPTDGEPYPFWHLHAHFYPPLLRSATVRKFM
                     VGYELLGQPQRDITPESAAARLRALPEEWP"
     gene            744..1910
                     /gene="galK"
                     /locus_tag="PK829_09785"
     CDS             744..1910
                     /gene="galK"
                     /locus_tag="PK829_09785"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOU41547.1"
                     /translation="MNPLNLTERFTAAFGAPPQLIVRSPGRVNLIGEHTDYNDGWVFP
                     AALDMGTVIAARRREDGQLRSVAARLGEEDAAPLTDLRPREEPGWTRYIRGVAALLLA
                     DGQPLSGADLLIDGDLPLGSGLSSSASLELGVATALLGLAGVAIERTALARLGQRVEN
                     EIVGLQSGIMDQLAVAGGVAGHALLIDCRSYAVRPVALPASVRVLVLDSAAPRTLAGS
                     AYNQRRAECESAVGKLQAARPEIRALRDVDAALLAAEGWRLDPVELRRARHVVSENQR
                     VLDSVAALEAGDVARFGALMVASHRSLRDDYAVSGPELDALVAIALDTPGVLGARLTG
                     AGFGGCAVALAAAADAEAAAAAITARYRAATGRDGRVYVSAAADGVAIWENRAT"
     gene            1926..3212
                     /locus_tag="PK829_09790"
     CDS             1926..3212
                     /locus_tag="PK829_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOU41548.1"
                     /translation="MSFQLLSPREQLTAIMQRIYRYGMTTLSGGNLSILDDNGDIWIT
                     PAGIDKGKLTPNDIMRVRSGGTAEGPHRPSTELPFHRAIYRLRPDVRAIVHAHPPALV
                     AFSIVRQVPDTRIVPQAGRFCGPVGYAPYDLPGSESLGERIAAAFATGSNVIILENHG
                     MAAAGPTLLDAFQRLETLEFCARIHLRARTLGDVLTLTEPALSLFDQCDNTLPEFTPA
                     AHSSAERELRRQVIDIAARAYERQLMISTQGVVSARLDADSFLITPAGRDRLTLGVED
                     VVLVRGGRREAGKLPSRAVLLHEAIYAANPDIAAIIAAQPPNVTAYAVTLAQFDSRTI
                     PESFILLRDIPRLPYEMCYTQLEAVAKRVSLRAPVALMANDGVLAVGNSVLAAFDRLE
                     VAEFSARSLIDAAVLGELAPMEAEAIEGLKAAFNLS"
     gene            complement(3285..>4139)
                     /locus_tag="PK829_09795"
     CDS             complement(3285..>4139)
                     /locus_tag="PK829_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOU41549.1"
                     /translation="RLLATSVTLSAYDPVRDETFTWPVEPRVWSQWLRLTVDPAAPTA
                     APTLAVDKQLAAAYLDTLAAEMGDGRYLHEDETPEAIRAALLAGQPAAGARVYHRESR
                     HVVQSGETLSSIGYDTGIPYPWIQQANPGIDALTVGQEIVIPSPDALLPLPVVAGKRI
                     IVSMSEQRARVYENGQLKWDWPASTGIDSSPTAPGVFQIQSHEPNAYAGNWNLWMPSF
                     MGIYRPVPTSDFMNGFHGFPTRGQAQLLWTGDLGHRVTYGCILLSSENAQALYDWAEE
                     GVVVEVQR"
BASE COUNT          549 a         1473 c         1483 g          634 t
ORIGIN      
        1 ctcggcaatg cggcccagcg tggcggtttc catggccacg ccggccaggc gctgcgtgat
       61 gtcgtcgtgc agctcgcgcg ccagccgccg gccctcgtcc tcgtaggcgg tgatcagccg
      121 cccgcccagc gtatgcgcct cgcgttcggc gcgctggcgg cggtctatct gggcgtccgc
      181 gaggacgggc gttggctgtg ggcggcgggg gcgctggcgg ggctggccat gttcacccgc
      241 aacgacggcc tggcgctgat cgcggcgggg gcgctcggcg ggctgtggct ggcctggcgg
      301 cgcggcgcgt atcctcgcct ccggcacgtg ctgggcgcgg gcgtcaccct ggcgattgtg
      361 ctgctgccct gggtggcgct ggcgcccttc tgggcggtat ggccctatga gacgctgctg
      421 ctgcctcgcg gccactgcgg cgcgttgact gacctgggcg acgccgcccg cgacgcgctg
      481 gccgacatcc tggggcggct aacgcggcgc tataaccgcg tcttcgacgt gcccttccct
      541 tacagcatgg gcttccacca gcagccgacg gacggcgagc cgtacccgtt ctggcacctg
      601 cacgcccact tctacccgcc gctgctgcgc tcggccaccg tgcgcaagtt catggtcggc
      661 tacgaactac tcggccagcc ccagcgcgac attaccccgg agagcgccgc cgcgcggctg
      721 cgcgccctgc cggaagagtg gccatgaacc ccctgaatct cactgagcgc ttcacggccg
      781 cctttggcgc gccgccgcag ttgatcgtcc gctcgcccgg ccgcgttaat ctcattggcg
      841 agcacaccga ctacaacgac ggctgggtct tcccggcggc gctggacatg ggcacggtca
      901 tcgccgcccg ccgccgcgag gacggccagc tgcgcagcgt ggccgcgcgc ctgggcgagg
      961 aggacgccgc gccgctgacc gacctgcgcc cgcgcgaaga gccgggctgg acgcgctaca
     1021 tccgcggcgt ggccgccttg ctgctggccg acggccagcc gctgagcggc gcggatctgc
     1081 tcatcgacgg cgacctaccc ctcggctcgg gcctgtcgtc gtcggcgtca ctggagttgg
     1141 gcgtggccac ggcgctgcta ggcctggccg gcgtggccat cgagcgcacg gcgttggcgc
     1201 ggctggggca gcgcgttgag aacgagatcg tcggcctgca aagcggcatc atggatcagt
     1261 tggccgtggc cggcggcgtg gctggccacg ccctgctgat cgactgccgc agctatgccg
     1321 tccggccggt ggcgctgccg gccagtgtgc gcgtgctggt gctcgacagc gctgcgccgc
     1381 gcacgctggc tggctcggcc tataaccagc gccgcgccga gtgcgagtcg gccgtgggca
     1441 agctgcaagc ggcgcggcca gaaatccgcg ccctgcgcga cgtggacgcc gccctgctgg
     1501 ccgccgaggg ctggcggctc gacccggtcg agttgcgccg cgcccgtcac gtcgtcagcg
     1561 agaaccagcg cgtgctcgac agcgtcgccg ccctagaggc gggcgacgtg gcccggttcg
     1621 gcgcgctcat ggtcgcctcc caccgcagcc tgcgcgatga ctacgcggtc agcggcccgg
     1681 aactggacgc gctggtggcg atcgccctgg acacgccggg cgtgctgggc gcgcggctga
     1741 ccggggcggg ctttggcggc tgcgctgtgg ccctggccgc cgcggccgac gccgaggcag
     1801 ccgccgcggc catcaccgcc cgctatcgcg ccgccacggg gcgcgacggt cgagtttacg
     1861 tcagcgccgc cgccgatggc gtcgcgattt gggagaatcg cgccacttag ccggagaaca
     1921 gcttcatgag ctttcaactt ctctcccccc gcgagcaact gaccgcaatc atgcagcgca
     1981 tctaccggta cggcatgacc acgctcagcg gcggcaacct gtccattctc gacgacaacg
     2041 gcgacatctg gatcaccccg gcgggcattg acaagggcaa gctgacgccc aacgacatca
     2101 tgcgcgtgcg gtcgggcggc acggccgaag ggccgcaccg gccgagcacc gagttgccct
     2161 tccaccgggc catctaccgg ctgcggccgg acgtgcgcgc catcgtccac gcccacccgc
     2221 cggcgctggt ggccttcagc atcgtccgcc aggtgccgga tacgcgcatc gtgccccagg
     2281 ccggccgctt ctgcgggccg gtcggctacg cgccctacga cctgcccggc agcgagtcgc
     2341 tgggcgagcg catcgccgcg gcctttgcca cgggcagcaa cgtcatcatc ctggagaacc
     2401 acggcatggc cgccgccggg ccgacactgc tcgacgcctt ccagcggctg gagacgctgg
     2461 agttctgcgc ccgcatccac ctgcgcgccc ggacgctggg tgacgtgctg acgctgacgg
     2521 agccggcact gagcctgttc gaccagtgcg acaacacgct gcccgaattc acgcccgcgg
     2581 cccacagcag cgctgagcgc gagttgcgcc ggcaggtcat cgacatagcc gcgcgggcct
     2641 atgagcggca actgatgatc agcacgcagg gcgtcgtctc ggcccggctg gatgccgata
     2701 gcttcctgat cacccccgcc ggccgcgacc ggctgacgct gggcgtggag gacgtggttc
     2761 tggtgcgcgg cggccggcgc gaggcgggca agctgcccag ccgcgctgtg ctgctccacg
     2821 aggctatcta cgccgccaac cccgacattg ccgccatcat tgccgcccag ccgcccaatg
     2881 tcaccgccta cgctgtcacg ttggcccagt tcgactcgcg caccatcccg gagagcttca
     2941 ttttgttgcg cgacatcccg cggctgccct acgagatgtg ctacacccag ctagaggccg
     3001 tggccaagcg cgtgtcgctg cgcgcgccgg tggcgctgat ggccaacgac ggcgtgctgg
     3061 ccgtcggcaa tagcgtcctg gccgcttttg accgtttgga ggtggccgag ttcagcgccc
     3121 ggtcgctgat tgacgcggcc gtgctgggcg agttagcgcc gatggaggcc gaggcgattg
     3181 aggggctgaa ggcggccttc aacctgagct agaagccgag ggggcagagg tctgccgtcc
     3241 gcggtcggtg gtcagcggtc gtctggcggc agccggcaag ccgcctagcg ctgcacctcg
     3301 accaccacgc cttcctcggc ccagtcgtag agcgcctgcg cgttctccga actgagcagg
     3361 atgcagccat aggtgacgcg gtggcccagg tcgcccgtcc acagcagttg ggcctggccg
     3421 cgcgtcggaa agccgtggaa accgttcatg aagtccgacg tcggcacggg ccggtagatg
     3481 cccatgaacg acggcatcca caggttccag ttgccggcgt aggcgttcgg ctcgtgggac
     3541 tgaatctgga agacgcctgg cgcggtgggg ctgctgtcga tgcccgtgct ggccggccaa
     3601 tcccacttga gctggccgtt ctcgtagacc cgcgcgcgct gctcgctcat gctgacgatg
     3661 atgcgcttgc cggcgacgac cggcagcggc aacagtgcgt cgggcgaggg gatgacgatc
     3721 tcctggccga cggtcagcgc gtcgatgccg gggttggcct gctggatcca cgggtagggg
     3781 atgccggtgt cgtagccgat gctcgacagc gtctcgcccg actggacgac gtggcggctc
     3841 tcgcggtggt agacgcgcgc cccggcggcg ggctgcccgg ccagcagggc ggcgcgaatg
     3901 gcctccgggg tctcgtcctc gtgcaaatag cggccgtcgc ccatctccgc cgccagggtg
     3961 tccaggtagg cggcggcgag ttgcttgtcc acagccagcg ttggcgcggc tgtgggcgcg
     4021 gccggatcga cggtcaggcg cagccactgg ctccagacgc gcggctcaac cggccaggtg
     4081 aacgtctcgt cgcgcacggg gtcataggcc gacagggtga cgctcgtggc cagcaggcg
//
LOCUS       DAOSMQ010000281         4139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21638_length_4139_cov_4.559256, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000281 DAOSMQ010000000
VERSION     DAOSMQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21638_length_4139_cov_4.559256"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="PK829_09800"
     CDS             complement(<1..1756)
                     /locus_tag="PK829_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU41550.1"
                     /translation="MSAETEIAQLQAALAALEAQRSLLGDAVVDAAVGPIRDSLAALY
                     ARARAGQQRKQATVLFADVYGFTTLSETMDAEDVASIINELWAVVDRAILDHGGYIDK
                     HIGDAVMAVWGARDAREDDPERAVRAALAMQMAVAHFCQTHHAPLALRIGINTGPVIL
                     GPVGTTGEFTAMGDAVNLASRLEHAAPVGGILISHDTYRHVRGIFDVQPPAPLTVKGK
                     AEPVQTYIVLRAKPRAFRMATRGVEGIETRMVGREAELDALRQAFTDAVGASQTCVAT
                     VIGEAGVGKSRLLYEFDNWIELRPEEITYFKGRGTPNTQHVPYSLFRDLFALRFDILD
                     SDSAAVALEKFQAGMAGFLDPDHAAIAGHWLGFDFSASEAAGRLLGTAGFAQAAQAYL
                     TRYFRRVVAEGPAVVFLEDIHWADDPSLDLTQHLVAALPAAPLLVVALARPAFFERRR
                     GWGEDSAAFRAVRLSPLSREAARALVDEILQRADAIPASLRDLIVNSAEGNPFYVEEL
                     VKMLIEQGVIERGMRNEELGIRNEERGSGGAGEQGGKEPPGGVAFSPAPLPPGSTALE
                     ARWTVRTDRLAALKVPATL"
     gene            complement(2004..3248)
                     /locus_tag="PK829_09805"
     CDS             complement(2004..3248)
                     /locus_tag="PK829_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41551.1"
                     /translation="MLKKAVATLFVCALLAGIFLAARPAQAEANADPYEPNNRRADAT
                     PITYGTRLNGLLINPAGDVDVFSFHGTAGDVINASAYPGGGDIPANPSLDLMKADGTI
                     LIHSDWQLDFDPTASFYYQLQATGTYYLRVLESGHPNAGGPDYDYDLTLNLVDTPPTG
                     LYVYVTTRAAGTVANISFDKRDILSWTDGVWSLFFDGSQKGLPARADFNAIDIDNAST
                     GSIVAAFVNPVSLPGAGMITPFDLAFYDGNNWSMHFDGSDVGLTQAGEKIDALEVLDS
                     SPGEFQPGQLNCLKVLWLSTTGSGKVTDYQGHALRFGREDILGFCVTQFGSHTQGQWF
                     PVQNYLPGNIDGLTVINSTPYMTTNSSFTLDGVAVGRSMIYREDLTNLSNGQPEGVLS
                     GPYWRAADAGLTVNIDGIDMIH"
     gene            complement(3645..>4139)
                     /locus_tag="PK829_09810"
     CDS             complement(3645..>4139)
                     /locus_tag="PK829_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41552.1"
                     /translation="LTSVLDTLSGLGFGVMGVALAIAVMRYRLFDIDVLIRKTTSYAI
                     LTALLALVYFGSVVVLQRVLSPVTGESTAAVVLSTLLIAALFLPLRRRVQGLIDRRFF
                     RKKYDAEQVLARFAATARDETDLDALTTELLRVIQETMEPETVSLWLRPVESRLPPLG
                     RGAE"
BASE COUNT          753 a         1278 c         1392 g          716 t
ORIGIN      
        1 tcagcgtcgc cgggaccttc agggcggcca gcctgtctgt ccgcaccgtc cagcgcgctt
       61 ccagcgcagt ggagccggga ggcagggggg caggggagaa agcaacccca cccggcggct
      121 ctttccctcc ctgctccccc gctcctccgc tcccccgctc ttcattccta attcctaatt
      181 cctcattcct cattccccgc tcgatgaccc cctgctcgat gagcatcttg accagttcct
      241 cgacgtagaa cgggttgccc tcggccgagt tgacgatgag atcgcgcagg ctggcgggga
      301 tggcgtcggc gcgctggagg atctcatcga ccagcgcccg ggcggcctcc cgcgacaggg
      361 gcgacaggcg cacggcgcga aaggccgcgc tgtcctcgcc ccagccgcgg cggcgctcga
      421 agaacgccgg ccgggccagg gccaccacca gcaacggcgc ggccggcagg gcggcgacga
      481 gatgctgggt caggtcgagc gatgggtcgt cggcccagtg gatgtcctcc aggaagacga
      541 cggccggccc ttcggcgacg acgcggcgga aatagcgcgt cagataagcc tgcgccgcct
      601 gggcgaagcc ggccgtgccc agcagccggc cggcggcctc gctggccgag aagtcgaagc
      661 ccagccagtg gccggcgatg gcggcgtggt cggggtcgag gaagccggcc atgcccgcct
      721 ggaacttctc cagggccacg gcggcgctgt cgctatcgag gatatcaaag cggagagcga
      781 ataggtcgcg gaagaggcta taggggacgt gttgggtgtt gggcgtgccg cggcccttaa
      841 agtaggtgat ctcctccggc cgcagctcga tccagttgtc gaactcatag agcagccggc
      901 tcttgcccac gcccgcctcg ccgatgacgg ttgcgacgca ggtctgcgat gcgccgacgg
      961 cgtcggtgaa cgcctgccgc agcgcgtcca actcggcctc ccggccgacc atgcgcgtct
     1021 cgatgccctc gacgccgcgg gtggccatgc ggaaggcccg cggcttggcc cgcaggacga
     1081 tgtaggtctg caccggctcg gccttgccct tgacggtcag cggcgcgggt ggctgcacat
     1141 cgaagatgcc acggacgtga cgataggtgt cgtggctgat gaggatgccg ccgaccgggg
     1201 cggcgtgctc caggcggctg gccaaattca cggcgtcgcc catcgccgtg aactcgcccg
     1261 tcgtccccac cggccccagg atcaccggcc cggtattgat gccgatgcgc aaagccaggg
     1321 gcgcgtggtg ggtctggcaa aagtgggcca cggccatctg catcgccagc gctgcccgca
     1381 cggcccgctc gggatcgtcc tcacgggcgt cgcgcgcgcc ccagacggcc atgaccgcgt
     1441 cgccgatgtg cttgtcgatg tagccgccgt ggtcgaggat ggccctgtct acgacggccc
     1501 acagctcatt aatgatgctg gccacatcct cagcatccat cgtttccgag agcgtcgtga
     1561 agccgtagac gtcggcgaac agaacggtgg cttgcttgcg ctgctggccg gcccgggcac
     1621 gggcgtacag ggctgccaga ctgtcgcgaa tggggccgac agcggcatcg acaacggcat
     1681 cccccagcag tgaccgctgg gcctcaaggg cggccagggc cgcctgcaac tgggcaatct
     1741 cggtttcggc tgacatgccc gcttctcctg ccggatagta tagcacaggc gggcgcggag
     1801 gctgcgtggc tgagggcggc ggccgtgtgt cgttcatgga tgggcctcga tcgcgagtga
     1861 cgaaacgggc cgctatcgcc gggcatagaa ggcaattggc ccctgctcgt tatgaagaga
     1921 gcgaggcttc gtgtccgggg aactaaaaac gggcctgaga tcgtcaggcc cgttttcgtt
     1981 cgcggtggtt ggaaccaggg cgtctagtgg atcatatcga tgccgtcgat attgaccgtc
     2041 agtccggcgt cggccgcccg ccagtatggc cccgaaagga caccttccgg ctggccatta
     2101 gacaagttgg tcaggtcttc gcggtagatc atagagcgac caacggctac gccgtcgagg
     2161 gtgaacgaac tgttggtggt catatagggc gtggaattga taaccgtcaa cccatcgatg
     2221 ttgccaggga gatagttttg aaccgggaac cactggccct gtgtatggct accgaactgg
     2281 gtgacgcaga aaccgaggat gtcctctctg ccgaagcgca gagcgtggcc ctgatagtcg
     2341 gtaaccttcc ctgacccggt tgtgctcaac cacaacacct taagacaatt cagttgcccg
     2401 ggttgaaact cccccggact gctgtcaaga acctccagag catcgatctt ctcgccagcc
     2461 tgagtcaggc cgacgtcgga gccgtcgaag tgcatcgacc agttgttgcc gtcgtagaag
     2521 gccaggtcga agggagtgat catgcccgcg ccgggcagcg acaccgggtt aacgaaggcg
     2581 gccacgatgg aaccagtgct ggcgttatcg atgtcgatgg cgttaaagtc ggccctggct
     2641 ggcagtcctt tctggctgcc gtcgaagaac agcgaccaga caccatcggt ccagctcagg
     2701 atgtcgcgtt tgtcgaagct gatgttcgct accgtgcccg cggccctggt ggtgacgtaa
     2761 acgtagaggc cggtcggagg cgtgtcgacc aggttgagtg tcaggtcgta gtcatagtct
     2821 gggccacccg cattcggatg tccgctttcc agcacacgga gataataagt tcccgtggcc
     2881 tgtagttgat aatagaagga cgccgtcggg tcaaaatcaa gctgccagtc cgagtgaatc
     2941 aggatcgtac cgtcggcctt catcaaatcg agcgaggggt tggcggggat gtccccaccg
     3001 ccggggtagg cgctggcatt aatcacgtcc ccggccgtac cgtggaagga gaagacgtcg
     3061 acgtcgccgg caggattgat caacaggccg ttgagtctcg ttccataggt gatgggagtg
     3121 gcgtcggcac gccggttgtt cggctcatag gggtcagcgt tcgcttcggc ctgggccggg
     3181 cgggccgcca gaaagattcc ggcgagcagt gcgcacacaa acaaagtggc tactgctttc
     3241 ttcaacatga taacctccga catctttagc aaatcgtatt gcacatccga cagatgacgg
     3301 gtttccaggt tacgatgctc tctgtatata gggcgcgcgc cgtggggtaa gagccgtact
     3361 gctgtcccga cagacgggct ggcaagtggc gcgggcctcg ttgttaggtg aaggagggga
     3421 ctgggggttg tgggttgtgg gaaatgttca gacctctgca aacacctgcg gctgagtata
     3481 gcattgccgg aatagaaggg ttgtaaaatg cattacagat acaaagctcc gaaccgcaag
     3541 tttgagttcg acaaggctga cggctgacat gcccgcttct cctgccggat agcccaggcc
     3601 ggcgcggggc tgcgcggccc agcgcggccg ccgggcgggg gcggctactc cgcgcccctg
     3661 cccagcggcg gcaaccgact ttccaccggc cgcaaccaca ggctgaccgt ctccggctcc
     3721 atcgtttcct gaatgacgcg cagcaactcg gtcgtcagcg cgtcgaggtc ggtctcgtcg
     3781 cgcgccgtgg cggcgaagcg ggccaacact tgctcggcgt cgtatttctt gcggaagaag
     3841 cgccggtcga tgaggccctg cacccggcgg cggagaggca ggaagagcgc ggcgatgagc
     3901 agcgtggaca ggacgacggc cgcggtcgac tcgccggtaa cgggcgacaa cacccgttgc
     3961 aatacgacga cgctgccgaa atagaccagc gccagcaatg ccgtgaggat ggcatacgac
     4021 gtcgtcttgc ggatgagcac gtcgatatcg aacaggcggt agcgcatgac ggcgatcgcc
     4081 agggccacgc ccatgacgcc gaagcccagc cccgacagag tatcgagcac gctcgtcaa
//
LOCUS       DAOSMQ010000282         4134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21676_length_4134_cov_5.277274, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000282 DAOSMQ010000000
VERSION     DAOSMQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21676_length_4134_cov_5.277274"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK829_09815"
     CDS             <1..391
                     /locus_tag="PK829_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOU41553.1"
                     /translation="IGSGPVEGTDEPSAAPLAGTVWRWATFRDAKQDYVVPATSPYTL
                     EFGDDGTVVVVADCNTANGTYTVNSDGTLTIAIRATTLAACPSDSLGDSFIEYLNQAG
                     PFEVDEAGTLIIQLMADGGTMTFIPAS"
     gene            531..3035
                     /locus_tag="PK829_09820"
     CDS             531..3035
                     /locus_tag="PK829_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOU41554.1"
                     /translation="MTRAEPIRLPIGILLLLALLAALSACRQQTAETPTIAPAGTPTA
                     ATTSAAATPAPAADAWATVQARGRLVVGTSADYPPFAFYTEEFGLDGFDIALARALGE
                     QLGVAVEFSDMAFDGLGGALQVGQIDAAIAAISVSESRAAVVDFSRVYFVSEDAALAR
                     TDSGILLRSIADLAPLRVGVQDGSVFQTWLQEVGVDAGVLSANNILAYTTTDQAIADL
                     LAGRIDVVIADRLPLEVIAREQDTLAIIGRGLNAQQLAVALAKGSTLTAPINDALAAL
                     EADGTLAELAGRYLNLDAAELAPLPTPAPVAPEGTPRAALAAAGCIDAMALVAHLDLD
                     DNNMASPPSISPGTAFQTTWRVQNIGTCAWDSSYQLTPVGGNVPQARMGSRAVPLPAT
                     VPPGETIDLTVDLIAPLTPGIYQGFWSMRGPGGLLFGERLSVGITVPELATPTPAPTA
                     TASPDIRFTADRTTIRAGECVNFAWQVDGAAQVSFQAEGQPWQLNTVASSGNSVECPA
                     TTTTFTLRVSRLDGTLELRPLRIDVTPVANAPAIVFFTTTPSFQITAGQCVEARWQVE
                     GDVTTVRVARNGQVLWDGAPLNGTSRDCPPVGEASYTLDVTGPGGTATGRQNVTVLPA
                     PTAATATPLPGGGPVVNFFAATPGQIALGQCISLSWSAGGDVARVRILRNGGVLLDFA
                     LVSATLSDCPGGTGVFTYRVEASAADGRVAFQQASVTVGATAQGIGGVWRLTNINGAV
                     VVPGTEVTAIFGDGGNLSGSSGCSTYSAAYQINGSSLAIGSLTNSGQFCAQPVGVMEQ
                     EQLFLTVLAAASGYSVDGNQLTIRSGRGQLTFIRAG"
     gene            complement(3387..>4134)
                     /locus_tag="PK829_09825"
     CDS             complement(3387..>4134)
                     /locus_tag="PK829_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41555.1"
                     /translation="GLSMIGDKDIVVRGDRIVFRNSGLEGTVEGGASGRIMTEPVDGT
                     ENISLTATVAGIVHDASKQARPLPSDSTRVPVAAFQPFEETGTMLRLDTFASIQPLFY
                     LEQAVGFVLLAEVFQSPEERRLVYTLRPVTDDGGVEAADAPPSIDSAQVKATTPAFRL
                     ADSGALHTIRAHQDGNLGAKQGGHRGYLEFWVNGLPLGVRLLPDARFRFRGGPAKLTI
                     GAWPRRDETLYLTGEIVHINFDPINKCTPC"
BASE COUNT          635 a         1482 c         1383 g          634 t
ORIGIN      
        1 catcggcagc ggcccggtgg agggcaccga cgagccgtcg gccgcgccgc tggccggcac
       61 ggtctggcgc tgggccacct tccgcgacgc caagcaggac tacgtcgtgc cggcgacgtc
      121 gccctacacg ctggagttcg gcgacgacgg cacagtcgtc gtcgtggccg actgcaacac
      181 ggccaacggc acgtacacgg tcaacagcga cggcacgctg acgattgcca tccgggccac
      241 gacgctggcc gcctgcccgt ccgactcgct gggtgattca ttcatcgagt acctgaacca
      301 ggccggcccg ttcgaggtag acgaagccgg cacgttgatc atccagctga tggccgatgg
      361 agggacgatg acgtttatcc cggcctcgta gctcatcgcc tcggcttacc gggcagtacc
      421 cggggcgggc gccaaccggc cgcatgcgac cggctatccc gccccggccc tgcctggccg
      481 ccccttctcg ccattccagc gccccggtcg cggcgctaat gaggtaaccc atgacgcgcg
      541 ctgaacccat tcgccttcca attggcattc tgttgctgtt ggcgcttctg gccgctctat
      601 ccgcctgccg ccaacagacg gctgagaccc cgaccattgc acccgccggc acgcccaccg
      661 ccgccaccac cagcgccgcc gccacccccg cccccgcggc cgacgcctgg gcgacggtgc
      721 aggcgcgtgg ccggctggtc gtcggcacgt cggccgacta cccgcccttc gccttctaca
      781 ccgaggagtt cgggctggac ggcttcgaca tcgccctggc gcgggcgctg ggcgagcaat
      841 tgggcgtcgc cgtcgagttc agcgacatgg ccttcgacgg cctgggcggg gcgctgcaag
      901 tcggccagat cgacgccgcc atcgccgcca tttccgtcag cgagtcgcgc gccgcggtgg
      961 tcgatttctc gcgcgtctac ttcgtcagcg aggacgccgc cctggcccgc accgacagcg
     1021 gcatcctgct gcgctccatc gccgacctgg ccccgctgcg tgttggcgtg caggacggct
     1081 cggtcttcca gacgtggctc caggaagtgg gcgtagacgc gggcgtccta tcggccaaca
     1141 acatcctggc ctacacgacc accgaccagg ctatcgctga cctgttggcc gggcgcatcg
     1201 acgtcgtcat cgccgaccgg ctgccgttgg aggtgatcgc gcgggagcag gacacgctgg
     1261 ccatcatcgg ccgcggcctg aacgcgcaac aactggccgt cgccctggcc aaaggctcca
     1321 cgctgaccgc gcccatcaac gacgccctgg ccgcgctgga ggccgacggc acgctggccg
     1381 aactggcggg gcgctacctg aacctggacg cggccgaact ggccccgctg cccacgccgg
     1441 cgcctgtggc cccggagggg acgccgcggg ccgctctggc cgccgccggc tgcatcgacg
     1501 cgatggccct ggtggcccat ctcgacctgg acgacaacaa catggcctcg ccgccgtcca
     1561 tcagcccagg cacggcgttc cagacgacct ggcgggtgca gaacatcggc acgtgcgcgt
     1621 gggacagcag ctaccaacta acgcccgtgg gcggcaacgt gccccaggcg cgcatgggca
     1681 gccgggccgt gcccttgccc gccacggtgc cgccgggcga gacgatcgat ctgaccgtcg
     1741 atctcatcgc cccgctgacg ccgggcatct accagggctt ctggtcgatg cgcgggccgg
     1801 gcgggctgct gttcggcgag cgcctctcgg tcggcatcac tgtgcccgaa ctggccacgc
     1861 cgacgccagc gccgacggct acggcctcgc ccgacattcg tttcaccgct gaccggacga
     1921 ccatccgcgc cggcgagtgc gtcaacttcg cgtggcaggt cgatggcgcg gcccaggtgt
     1981 cgttccaggc tgagggccag ccctggcaac tcaacaccgt cgccagcagc ggcaacagcg
     2041 tcgagtgccc cgcgacgacg acgaccttca ccctgcgcgt cagccggctc gacggcacgc
     2101 tggagctgcg gccgctgcgc atcgacgtga cgcccgtggc caacgcgcca gccatcgttt
     2161 tcttcacgac cacgcccagc ttccagatca ccgccggcca gtgcgtcgag gcacgctggc
     2221 aagtggaggg ggacgtgacg accgtgcgcg tggcccgcaa cggccaggtg ctctgggacg
     2281 gcgcgccgct caatggcacg tcgcgcgact gcccgcccgt gggcgaagcc agctacacac
     2341 tcgatgtgac cgggccgggc ggcacggcca ccggccggca gaacgtcacc gtgctgcccg
     2401 cgcccacggc ggccacggcc acgccgctgc ccggcggcgg gccggtcgtc aacttcttcg
     2461 ccgccacgcc cggccagatc gccctggggc agtgcatcag cctcagttgg agcgccggcg
     2521 gcgacgtggc ccgcgtgcgc atcctgcgca acggcggcgt gctgctcgac tttgccctgg
     2581 tcagcgccac gctgagcgac tgccccggcg ggacaggggt cttcacctac cgcgtcgagg
     2641 ccagcgccgc cgacggccgc gtcgccttcc aacaggcgtc ggtgacggtc ggggcgacgg
     2701 cgcagggcat cggcggcgtc tggcggctga ccaacatcaa cggcgcggtt gtcgtgcccg
     2761 gcacggaggt aacggccatc ttcggcgacg gcggcaacct gagcggctcg tcgggttgca
     2821 gcacctatag cgccgcctac cagatcaacg gcagcagcct ggccatcggc tcgctgacca
     2881 actccggcca gttctgcgcc cagccggttg gcgtcatgga gcaggagcag cttttcctga
     2941 ccgtactggc ggcggcatcg ggctattcgg tggacggcaa ccagttgacc attcgcagcg
     3001 gccgggggca attgacattc attcgggccg gctaactgag tggaccttcg ggcggcacac
     3061 ccgcggtgcg ccgcccgcct gtccgaggca cgagagagag gagaaccgga cggcggctgt
     3121 tttgctgggc tgccgtcact ctggaggagg actggaacac ttacagtgta gattgcttta
     3181 gcgcgacccg tttcccgcga tccggcctct gattcccgtt tgccagagac aaagcggccg
     3241 gcaagtggtt ttgccgagcc acagaagctc acgcaaagaa cgccaaggaa gcaaaggaca
     3301 caaaggaaga agacttattc ttctttgcgc cttggcttac ttagcgcctt tgcgtgagct
     3361 ctgctttcga ctatgcaatg acccggttag cagggcgtac acttgttgat cgggtcgaag
     3421 ttgatgtgga cgatctcgcc ggtcaggtag agcgtctcgt cccggcgcgg ccaggcgcca
     3481 atggtgagtt tcgccgggcc gccacggaag cggaagcggg cgtccggcag caggcgcacg
     3541 cccaggggca gcccgttgac ccaaaactcc agatagccgc ggtggccgcc ctgcttggcg
     3601 ccgaggttgc catcctgatg ggcgcggata gtgtgcagcg cgccgctgtc ggccaggcgg
     3661 aaggcgggcg tcgttgcctt cacctgggcg ctgtcgatgg agggcggcgc gtcggccgcc
     3721 tccacgccgc cgtcgtctgt caccggccgc aaagtataga ccagccgccg ttcctccggc
     3781 gattggaaga cctcggccag cagcacaaag ccgacggcct gctccagata gaaaaggggc
     3841 tggatggagg cgaacgtgtc cagtcgcagc atcgtgcctg tctcctcgaa cggctggaag
     3901 gcggccacgg gcacgcgcgt gctgtcgctg ggcaagggcc tggcctgctt gctggcgtca
     3961 tggacaatgc cggccaccgt cgccgtcagg gagatattct cggtgccgtc caccggttcg
     4021 gtcatgatcc ggccgctcgc gccgccctcg accgtgccct ccaggccgct gttgcgaaag
     4081 acaatgcggt cgccgcgcac gacgatgtct ttgtcgccta tcatcgacag gcca
//
LOCUS       DAOSMQ010000283         4129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21699_length_4129_cov_3.815169, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000283 DAOSMQ010000000
VERSION     DAOSMQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21699_length_4129_cov_3.815169"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK829_09830"
     CDS             complement(<1..217)
                     /locus_tag="PK829_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU41556.1"
                     /translation="MDDRPRRVCPACGYVHFTDPKVGVGVLVVEGDRVLLVRRGVAPE
                     RGKWGLPAGFLDQGEDPRATAAREALEE"
     gene            complement(285..956)
                     /locus_tag="PK829_09835"
     CDS             complement(285..956)
                     /locus_tag="PK829_09835"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOU41557.1"
                     /translation="MKSDGCPPPTRSTSCGRTTPRWPNGKTEIITYMPPTEYHQQLVR
                     LLVVLVSSFVELVDAGVVHVAPFEVKLWPGGPSREPDILFIDRERLSQLTPQRFEGGP
                     DLVVEIVSSGSARIDRVEKFIEYERAGVREYWLIDPRPGKEQADFYGLEDNGRFASLG
                     LDDDGQFSSLVLPGLRLDPALLRGPLPNPQLLLAGIAKDLPSLREELRAAYRALYEAL
                     SQEPH"
     gene            complement(1246..2094)
                     /locus_tag="PK829_09840"
     CDS             complement(1246..2094)
                     /locus_tag="PK829_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41558.1"
                     /translation="MFNSMRDRGDRDRLQETLDAYLDNALTPAERARFEAQMTADPRL
                     RTEVEQLRALRWQMRAMPRRRVPRSFALDPAAYARPKAQPLLQLYPVLRGATALSAFL
                     LIFVLALGAFQGQFGGAGAPAAAAVTSTTAQDSAEIAAIEAPQQPPAEESAAMTADEA
                     ARTASPETTTVTEEAAAAELAAPAPAGTSVPVPEGDLSLGASPPEATLLPNAGGAIAE
                     AELAPTLAAEPTMELFAVQEAANAPAPEAVAQPSTLLLVQIGLGVLFLVLLALWLLAR
                     RRARSL"
     gene            complement(2155..2754)
                     /locus_tag="PK829_09845"
     CDS             complement(2155..2754)
                     /locus_tag="PK829_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU41559.1"
                     /translation="MNEETLIERAQKGDIAAFNRLVVQYQELVFNVAYRIMSDPAAAE
                     DVAQETFITAHQSLRSFRGGSFKSWLIRIATNRCYDELRRRKRRPQSSLDEIMDENES
                     FAFLRSPQDSPETHRQRVELALAIESCLKDLPDDQRVVTVLSDVEGYDYQEIANITRV
                     SLGTVKSRISRARAKLRDCLQSLGGELLPVAYRLDSKGT"
     gene            2855..2936
                     /locus_tag="PK829_09850"
     tRNA            2855..2936
                     /locus_tag="PK829_09850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2889..2891,aa:Leu,seq:caa)
     gene            complement(3025..3459)
                     /locus_tag="PK829_09855"
     CDS             complement(3025..3459)
                     /locus_tag="PK829_09855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HOU41560.1"
                     /translation="MREDDLSLFGFENEDDLDLFTVLLGVQGIGPKVALSILGTLSPE
                     LLKSAIMREETAVLQRVPGIGKKTAERIMFQLRDKLDLGGAAATMPLVSDIDADVIDV
                     LTGLGFSIIEAQSALQHVPREVRAMDERVQAALQYLDQTGQR"
     gene            complement(3785..>4129)
                     /locus_tag="PK829_09860"
     CDS             complement(3785..>4129)
                     /locus_tag="PK829_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU41561.1"
                     /translation="HQLRDLLATHRPATAGVEQLFFGRNITTAIAVGQARGVLLLALA
                     EAGVPVSEYSPPKVKEAVTGYGKADKAQVQLMVRHILDLAETPHPDDAADALAVAIAH
                     AQFESFRQALER"
BASE COUNT          680 a         1377 c         1358 g          714 t
ORIGIN      
        1 cctcctccag cgcctcgcgg gcggccgtcg cccgtgggtc ttcgccctga tcgaggaagc
       61 cggccggcag gccccattta ccccgctccg gcgccacgcc ccggcgcacc agcagcaccc
      121 ggtcgccctc caccaccagc acgcccacgc caaccttcgg gtccgtaaag tggacatagc
      181 cgcaggcggg gcagacgcgg cgcggccggt cgtccacgtg gcgcgtttcc agcggcgcgg
      241 cgcagcgcgg acagaaggca aaccaggcag gggcgctgtc ggcgttaatg gggttcctgg
      301 ctcagagctt cgtagagggc gcggtaggcg gcgcgcagtt cctcgcgcag cgacggcagg
      361 tctttggcga tgccggccag cagcaattgc ggattgggca gcggcccacg cagcagtgcc
      421 gggtcgaggc gcaggccggg cagcacgagc gaactgaact ggccgtcgtc gtccaggccc
      481 agcgacgcaa agcggccgtt gtcctccaac ccatagaagt cggcctgttc cttgcccggc
      541 cgggggtcaa tcagccagta ctcgcgcacg ccggcgcgct catactcgat gaacttctcc
      601 acgcgatcga tgcgcgcgct gccggacgag acgatctcga ccaccaggtc cggcccgcct
      661 tcaaagcgct gtggcgtcag ttggctcagg cgctcacggt cgatgaagag aatgtccggc
      721 tcgcgcgacg ggccgccggg ccagagcttc acctcaaacg gcgcaacgtg gacgacgccg
      781 gcgtcaacca actcgacaaa cgaactgacg aggacaacca acaggcggac gagttgttga
      841 tgatattccg tgggtggcat gtaggtgata atctccgtct ttccattcgg ccagcgtggc
      901 gtcgtccggc cacaggaggt actccgcgta ggtggcgggc agccgtctga cttcatcttg
      961 ctgcctatgc gcagcggtcg cttcccgtgt ccattcggtc atgggctttc catcctcggc
     1021 atgaggccat tatagcatag ctacggacat tggagagagg agggccgaac cacagtcagg
     1081 gtcattgcaa agtcatgaag acacctcacg caaagatcgc caagagggca aagagcgcaa
     1141 aaaggacggg ccttggcgtt cttagcttcc ttcgcgttct ttgcgtgagg tcttcttccg
     1201 agtttgcgac agccctggaa accacagcgc ggactgtcgt cgccgctaca gcgaccgcgc
     1261 ccgccgccgg gccaagagcc acagcgccag cagcaccagg aacaacaccc ccagcccgat
     1321 ttgcacgagc aggagcgtag acggctgcgc caccgcctct ggcgcgggcg cgttggccgc
     1381 ttcctggacg gcgaagagct ccatcgtcgg ctcggccgcc agggtcggcg caagctcagc
     1441 ttccgcgatg gctccgccgg cgttgggcag cagcgtcgcc tccggcggag acgctcccag
     1501 cgacaggtcg ccttccggga cgggcacaga tgtgcccgcc ggggccggcg cggccagttc
     1561 ggcggcggcg gcttcttcgg tgacggttgt cgtctctggc gaggccgtgc gcgcggcctc
     1621 gtcggcggtc atggccgcgc tctcttcggc cgggggctgt tgcggcgctt cgatggccgc
     1681 gatctcggcc gaatcctggg ccgtggtgct ggtgacagcg gccgcggccg gcgcgccggc
     1741 gccgccaaac tgcccctgga acgcgcccag ggccaggacg aaaatcagca ggaaggccga
     1801 cagcgccgtg gccccgcgca gaacggggta gagttgcagc agcggctggg ccttggggcg
     1861 ggcgtaggcg gccggatcga gggcgaaact gcgcggcacg cgccggcgcg gcatggcccg
     1921 catctgccag cgcaaggccc gcagttgctc cacctccgtc cgcaggcggg ggtcggccgt
     1981 catctgcgcc tcgaagcgcg cccgctcggc cggcgtgagg gcattgtcca ggtaggcgtc
     2041 cagcgtctcc tggagtctgt ctctatcgcc gcgatcccgc atcgagttga acattggttt
     2101 cacatacaga cctgaccagc ctcgcccggc cggtcaggtc atggttcttc cggtctaagt
     2161 tcctttagaa tctagacgat aggccaccgg taatagttcc ccgccgaggg actgcaaaca
     2221 gtcgcgcaac ttggcccgcg cccggctgat gcgcgacttg accgtgccca gcgagacgcg
     2281 ggtgatatta gcgatctcct ggtagtcgta gccctcgaca tcgctcagga cggtgacgac
     2341 gcgctggtca tcgggcaaat ctttcaggca gctttcgatg gccagggcca actcaacgcg
     2401 ctggcggtgg gtctccgggc tgtcctgggg gctgcgcagg aaggcaaagg actcattctc
     2461 gtccatgatc tcgtcgaggg aggactgcgg ccgccgcttg cgccgccgca gctcgtcgta
     2521 gcagcggttg gtggcgatgc ggatgagcca cgacttgaag ctgccgccgc ggaaggaacg
     2581 cagactctgg tgggcggtga tgaacgtctc ctgggcaacg tcttcggccg cggccgggtc
     2641 gctcatgatg cgataggcca cgttgaagac aagctcctgg tactggacga ccaggcggtt
     2701 gaaggcggcg atatcgcctt tctgcgccct ctcgatgagg gtttcttcgt tcatacactc
     2761 actgcctgct ggcgctggcg tccggccgcg cgggactacc cttcaattga cagccatccg
     2821 tccttcctct atactccccc ttgagaacga ctgcgccgaa gtggcggaac aggcagacgc
     2881 gcacgactca aaatcgtgtt ccctcgggag tgtgggttcg attcccacct tcggcatcga
     2941 ttatagtctg gccggcaagg cgggcaaacc taagggcttc catcgcggaa gccttttctt
     3001 tttggggggt tggggcgcgc cgctctagcg ttgcccggtc tgatcgagat attgcaaagc
     3061 cgcctgcacg cgctcgtcca tcgcccggac ttcgcgcggc acgtgctgca aggccgattg
     3121 cgcctcgatg atgctgaagc ccaggccggt cagcacgtcg atcacatcgg cgtcgatgtc
     3181 gctgaccagc ggcatggtcg ccgccgcgcc gccgaggtcg agcttgtcgc gcagttggaa
     3241 catgatgcgc tcggccgtct tcttgccgat gccgggcacg cgctgcaaga cggccgtctc
     3301 ctcgcgcatg atggcgctct tgagcagttc cgggctgagc gtgcccagga tggacagggc
     3361 caccttcggg ccaatgccct gcacgcccag cagcacggtg aacagatcca ggtcgtcctc
     3421 gttctcgaag ccgaagaggg ataggtcgtc ctcgcgcacc tgcaagtggt gtacaggcca
     3481 atggcccggc cgatgccgcc gacgttctcc aataccgtcc ggggcacaaa cacgcgcaac
     3541 cccaccccgc cgacagccac caccaggctg ctggcctcga tcttctgcac tgtcccgcta
     3601 atgctggcga tcatggcttt ggcaattacc ctgctgaatt atggacgacg gacgacagac
     3661 gacgggccac ggcttgcagg gcagactcgc tggccgcgaa gcatgctttc tggccgtcgt
     3721 ccgcggtcgg tcgtctgccg tccctcccca ctgtccactg tccactactt actgcccact
     3781 ccctttatct ctccaacgcc tgccggaacg actcaaattg cgcgtgggcg atggccacgg
     3841 ccagggcgtc ggcggcgtcg tcgggatggg gcgtctcggc caggtcgagg atgtgacgaa
     3901 ccatcaactg tacctgggcc ttgtccgcct tgccatagcc ggtgacggct tccttgacct
     3961 tcggcggcga gtactcgctg acgggcacgc ccgcctcggc cagcgccagc agcaacaccc
     4021 cccgcgcctg ccccacggcg atggccgtgg tgatgttgcg gccgaaaaag agctgctcga
     4081 cgcccgccgt cgctggacgg tgggtggcga gcaggtcgcg aagttgatg
//
LOCUS       DAOSMQ010000284         4122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21743_length_4122_cov_3.378657, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000284 DAOSMQ010000000
VERSION     DAOSMQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21743_length_4122_cov_3.378657"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2344)
                     /locus_tag="PK829_09865"
     CDS             complement(<1..2344)
                     /locus_tag="PK829_09865"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOU41562.1"
                     /translation="MTSKNPSDRRAVNKWLALPVVAAAGALGATVATRRLMHRPVPKP
                     SGRAILAGLRAPVEVIRDAWGVPHIYAANNDDLFFAQGYVQAQDRLFQMDLNRRLGLG
                     RLAEVTGPLGVTFDKFARYLGWPRAARAQADLSDAATAAVITAYSAGVNAFIASQPLP
                     AEFRLLAYRPEPWDALATSAWGTVLGWGLSCNWESELLRAWLLDALGPERAADLTPVY
                     RPDYATTLPNEGVGRRLAEGLLGAFRETVAHGPVGAPLIGQSLGSNNWVVGGARTASG
                     RPHLANDPHLPPVFPTIWYANHLCGGDYHVTGFTLPGVPGVVIGHNEHCAWGITNAFP
                     DVQDVYIERFDPNDPTRYKVNGTWQAAEVTQEIIHVRGLRDVVITVRTTRHGPVFSDL
                     LPGGPAVPSDSAVPNHHADLAYDWTLFRSGNHLRAVLATNRASTWESFREGLRHWSFP
                     GQNVVYADTTGDIAYMMPGLVPRRRNGNGLTPVPGWNDNFAWDGFIPFDELPYYVNPP
                     EGFIATANNCMTGDSYPHWLTGEWLTDYRVRRIRALLAERPGLTLDDHRRIQTDTVSL
                     LARRFLGAALPVAQPAAQMAAQATAPAAAGLRDALARLAAWDGDMAEDSIAATLQFGW
                     LVHFTRAAVAQAVGPQRAETLLARGEQTGFPFMPFYEIAYELALRWLEGASGLAGGAP
                     PPDWVGDVRPLLLPALRETLAVLRREVGPDPAAWTWGRLHRVAFQHEMTRLPGVGRLW
                     KPTTISAPGDGYSINQSDLSPHFPPDPATIIASCRLIVDVG"
     gene            complement(2322..3218)
                     /locus_tag="PK829_09870"
     CDS             complement(2322..3218)
                     /locus_tag="PK829_09870"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU41563.1"
                     /translation="MSDPIPVCDYEGSDYHARFWQNQGRDYEDRAERVALRRLMPPRG
                     DTLLDVGAGFGRLADEYAGYRRVVLFDYSRSLLREARQRWGDDPRFFYVAGNWYHLPF
                     VDDLFETLVQVRTLHHAADPPALFDELARVARADAAYVLEFASKHNVKAMLRYWLGRQ
                     TWSPFDPQPVEFVALNYDFHPDWIREQLAMAGFAPGDMLTVSHFRLGPLKRLAPTGLL
                     VALDSAAQRTGRWWQFSPSVFVRSRLVRPPSPLAIGGFFACPTCHLSLGEPRDGLLVC
                     ANPACGRRWRVEDGLYDFKEPV"
     gene            3462..>4122
                     /locus_tag="PK829_09875"
     CDS             3462..>4122
                     /locus_tag="PK829_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOU41564.1"
                     /translation="MANDVAIFLDLDNLVIGARDANLSFDVNLIIDDIKRRTGGRVVL
                     RRAYSGNLRQDQKLMKDIAAAGFTMQSAVSLNNFGKNLIDMYMVVDTMDTLVDGQQYS
                     TYVLVTGDRDFLPLIHALRRRGKHVIGIGLRHTTSENLMSLCDEYIYYEDLLPAPPLN
                     DSETEVLLAKAVDSLLVGDERVRASVVKERMIELSEGRFGRLQYTDTSFSKFLSRYPS
                     RI"
BASE COUNT          761 a         1313 c         1438 g          610 t
ORIGIN      
        1 cgccgacatc gacgatgagc cggcagctgg cgatgatcgt cgccggatcg ggcgggaagt
       61 ggggcgacag atcgctctga ttgatgctat agccgtcgcc gggggcggag atggtcgtcg
      121 gcttccatag acggccgacg cccggcagcc gcgtcatttc gtgctggaag gcgacgcggt
      181 ggagccggcc ccacgtccag gccgccgggt cggggccaac ttcgcgacgc aggacggcca
      241 gcgtctcacg cagggcgggc agcagcagcg gtcgcacgtc gcccacccag tccgggggcg
      301 gcgcgccgcc ggccagcccc gacgccccct ccagccagcg cagcgccaac tcgtaggcga
      361 tctcgtagaa gggcatgaac ggaaagccgg tctgctcgcc gcgggccagc agcgtctctg
      421 cccgctgggg accaacagcc tgggccacgg cggcgcgggt gaagtgcacc aaccagccaa
      481 actggagcgt cgcggcgatg ctgtcctcgg ccatgtcgcc atcccaggcg gccagacggg
      541 ccaacgcgtc gcgcagcccg gccgcggctg gagccgtggc ctgcgccgcc atctgcgccg
      601 caggctgcgc caccggcagc gccgcgccca ggaaacgccg cgccaatagc gagacggtat
      661 cggtttggat gcggcggtgg tcgtcgaggg taagtccagg gcgctcggcc agcagggcgc
      721 ggatgcggcg gacgcggtag tcggtcagcc actcgcctgt cagccagtgg ggatagctgt
      781 cgcccgtcat gcagttgttg gccgtggcga taaaaccctc tggtgggttg acataatatg
      841 gcaactcatc gaaggggatg aagccatccc aggcgaagtt gtcgttccag ccaggcacgg
      901 gcgtcagccc attgccgttg cgccgccggg gaacgaggcc gggcatcatg taggcgatat
      961 caccggttgt gtcggcatag accacattct ggccggggaa cgaccaatgc cgcagcccct
     1021 cgcggaagct ctcccaggta ctggcccggt tagtagccag cacagcccgc aagtgattgc
     1081 ccgaccggaa gagcgtccag tcgtaggcca aatcggcatg gtggttgggg accgcggagt
     1141 cgctggggac agcagggccg ccgggcagca ggtcagagaa gacggggccg tggcgtgtgg
     1201 tgcgaacggt aatgacgaca tcgcgcaggc cgcgaacgtg gataatctcc tgagtcacct
     1261 cggccgcctg ccacgtgccg ttgaccttat agcgcgtcgg atcgttgggg tcgaagcgct
     1321 cgatgtagac atcctgcaca tccgggaagg cattggtgat gccccaggcg caatgctcgt
     1381 tgtggccgat gacgacaccg ggcacgccgg gcagggtaaa gccagtgacg tggtaatcgc
     1441 cgccgcaaag gtgattggcg taccagatgg tcgggaagac gggcggcagg tgggggtcgt
     1501 tggccaggtg cggccggccg ctggcggttc gcgccccgcc gacgacccag ttgttgctgc
     1561 ccaggctctg gccgatgagc ggcgcgccca ccgggccatg ggcaactgtt tcgcggaaag
     1621 cgcccaacag tccctcggcc aaacggcggc cgacaccctc attgggcaac gtcgtggcat
     1681 agtcggggcg gtagacaggc gtcaggtcgg cggcgcgctc cggccccagc gcgtcgagca
     1741 gccaggcgcg cagtaactcc gactcccagt tgcacgatag cccccagccc aacaccgtcc
     1801 cccaggcgct ggtggccagg gcatcccagg gttccgggcg gtaagccagc aagcggaact
     1861 cggccggcag gggctggctg gcgatgaagg cgttgacgcc ggcgctgtag gcggtgatga
     1921 ccgcggccgt cgccgcgtcg ctcaggtcgg cctgggcgcg ggcagcgcgc ggccagccca
     1981 aatagcgggc gaacttgtcg aaagtgacgc ccagcgggcc ggttacctcg gccagccggc
     2041 ccaaccccag ccgccggttg aggtccatct ggaagaggcg gtcctgggcc tggacgtagc
     2101 cctgggcgaa gaagagatcg tcattgttgg cggcgtagat gtggggcacg ccccaggcat
     2161 cgcggatgac ttccaccgga gcgcgcaggc cagcgaggat ggcgcggccg ctgggcttgg
     2221 gcacggggcg atgcatcagg cggcgtgtgg ccacggtcgc gcccagcgcg ccggcggcag
     2281 cgaccaccgg cagggccagc cacttgttga ccgcccggcg atcagacggg ttctttgaag
     2341 tcatacaggc catcctctac ccgccagcgc cggccgcagg ccgggttggc gcagaccagc
     2401 aggccgtcgc gcggttcgcc gagggagagg tggcaggtcg ggcaggcgaa gaagccgccg
     2461 atagccaacg gtgacggtgg ccggacgagg cggctgcgga cgaagacgct gggggagaac
     2521 tgccaccagc ggccggtgcg ctgggcggca ctgtcgaggg ccaccagcag cccggtcggg
     2581 gccagacgct tgagcgggcc gaggcggaag tgggatacag tcaacatgtc gccgggcgca
     2641 aaaccggcca tcgccagttg ctcgcggatc caatcggggt ggaagtcata gttgagggcg
     2701 acaaattcga ccggttgggg gtcgaagggc gaccacgttt gccggccgag ccagtagcgt
     2761 agcatggcct tgacgttgtg cttactggcg aattcgagca cgtaagcggc gtcggcgcgg
     2821 gcgacacggg ccagttcgtc gaacagcgcc ggtggatcgg cggcatgatg gagcgtgcgc
     2881 acctggacga gcgtctcaaa cagatcatcg acgaagggca gatgatacca attgccggcg
     2941 acatagaaga agcgtggatc atcgccccag cgctggcgag cctcgcgcag tagagagcgg
     3001 gagtagtcga acaggacgac gcggcgataa ccagcgtact catcggccag gcggccaaag
     3061 cccgcgccga catcgagcag cgtatcgccg cgcggcggca tcagccgccg cagagcgacg
     3121 cgctcggcgc ggtcctcgta gtcgcgcccc tgattctgcc agaagcgggc gtggtagtcg
     3181 gagccttcgt agtcgcagac ggggatggga tcagacatcg cacttgcggc cgccgggatt
     3241 aggcgcgacg gcgcgatagc gcatcgtagc acgtttgccg ggcaacgcca agagagggca
     3301 ctgtttcccg tgaaacattg gcttctgttt cccgtgaaac atcggtcgcc ttttccgttg
     3361 ccagtccgcc ggaataggct tagaatcgac ccatagaaga acaacagcca gccgacgacg
     3421 cgacacgcgt aacccgccgg gcctttcagg acaaatcacc catggccaat gatgtcgcca
     3481 tctttcttga cctcgacaac ctggtaattg gcgcgcgcga cgccaacctc agctttgacg
     3541 tgaacctcat catcgacgac atcaagcggc gcaccggcgg ccgggtcgtc ctgcgccgcg
     3601 cctactccgg caacctgcgc caagaccaga agctgatgaa ggatatcgcc gccgccggct
     3661 tcaccatgca atcggccgtg tccctcaaca acttcggcaa gaaccttatc gacatgtata
     3721 tggtcgtcga tacgatggat accctggtcg atggccaaca atatagcacc tacgtcctgg
     3781 ttaccggcga ccgcgacttc ctgccgctca tccacgctct gcggcggcgc ggcaagcatg
     3841 tcatcggcat cggcctgcgg cacacgacca gcgaaaacct gatgagcctg tgcgacgagt
     3901 atatctacta cgaagatctc ctgcccgcgc cgccgctgaa cgacagcgag accgaagtac
     3961 tcctggccaa ggccgtcgat agtctcctgg taggcgatga gcgtgtgcgg gccagcgtcg
     4021 tcaaggaacg catgatcgag ctgagtgagg ggcgcttcgg ccggctgcaa tataccgaca
     4081 catccttcag caagttcctc agccgctatc ccagccgcat cg
//
LOCUS       DAOSMQ010000285         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_21875_length_4103_cov_3.979990, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000285 DAOSMQ010000000
VERSION     DAOSMQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21875_length_4103_cov_3.979990"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..2159
                     /locus_tag="PK829_09880"
     CDS             393..2159
                     /locus_tag="PK829_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOU41565.1"
                     /translation="MKSIFRVWRLGVLVLTFLGFGAMSVAARADEIPVPSLWYSPLEI
                     HFGSVPIDGRVERQVDVHNLGTAPMTLSGGEVEKPFSIDYDTCGGVVAAGASCRLTFS
                     FAPTAAGDFTAEANGDSDAGPWHVVLRGRTAAPEITVSPRSLDFGRGLVGSAFPPQVV
                     VVTNIGGVPVGGFRAEPVSVPFTGGLGQCAAGLQPGQSCHMTFDFAAENAGVFHKFWT
                     AQSDAGPIEIELQGRTYTGIAGTGQGVTPRAIDFGPVRVGDTIRQTVTFHNFDPSIPI
                     VNWDYSWITDDPYIDFDYQTNCGESLPGLAECKVTISYRPRDFGDDTVILNVLNSQGI
                     IDINLWGQGAAAEIVADSPAIDLGMATDGRGSDQVVRFTNLGHGPADVLGIESAPSFI
                     ISDSDCGDVLAPGQSCRAWVRFDPNGYGRREEEIALLTGADPVPVRVVGGLLTPQLAV
                     AFAPRAATVGDLVHLRLTFSNANPAQALFDLGLDGQLPGGLALLDGPPEASANCGGPT
                     LTRAANGTEFSLRDMTLAAGDTCIVDLAAQAVLAGQWRFDSAAYSHAGPSQLSGDSLV
                     VTDDEETFTYQLFLPSLLGPTN"
     gene            2177..2851
                     /locus_tag="PK829_09885"
     CDS             2177..2851
                     /locus_tag="PK829_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOU41566.1"
                     /translation="MHIDVFSDTVCPWCRIGKRHLELALAGWGGSPAEPVTVRYRSFF
                     LDPTIPPEGRDFRDHMLAKGGGRVALEDFFAAPRERGAAVGLSFNFEAIQRAPNTLLS
                     HRLVYLAPEARRGPVLDALYEAYFARGEDIGDVATLAVVAERAGLDGPTVVAQLATTA
                     GTAEVWADVDYARRAGIRGVPFFIFNERLAFSGAQPPEVIQRVLHQAAAEASQAQPRD
                     QGKLTQ"
     gene            2928..3941
                     /locus_tag="PK829_09890"
     CDS             2928..3941
                     /locus_tag="PK829_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41567.1"
                     /translation="MSTRRVAQAAMGVGALVVALFLVSAWARPAAAVGGGSGRWLQMM
                     SNDGGVGPNDIAGPDDDPVVAFTRPGSVSPGCDAPTVHYGVGDAVLYFARHRCWLPWF
                     DGEGSGLPAGADINALHDECGPTEPRCTIYLSFTRTLRVPGVGKVQPHDIVSADWVEG
                     TQDIYTNFWLVFDGSDVGLTEPGERIDALFLFDEDRPEPYRDCVALGWASTVGNYRVR
                     DQWGGWLTGGGEDILGFCASSIGEDTSGYWFLYHDGSAEGLPRNALVGLAHEEGRAAS
                     GRFNFLTKGPFQADEADGGAGDIFEFSSYNGGMYRGPLVSFWDDLGVAGVPDSIHIYY
                     THE"
BASE COUNT          635 a         1389 c         1387 g          692 t
ORIGIN      
        1 gggcaagccc atcgagcggc ggcgactgga aggtttcgac tacggcagct tcgcgccgga
       61 gagccagcgg cccgcgccga agaagagtct ctatcgctcg cgcggcttcc gcgggcagga
      121 acgcggcggg ctgccggcac agtcgcggcg gtagttgacc ggggcgcgcc cggacgcgcg
      181 ggcgcatctg tgcgggcgta gggctttgcc acagttgtcg agcgaccatt gacctgatag
      241 gtgggcggcc acgaatggca cgtccacctg tcaggtcggt gcgccgggcg tgactttggc
      301 aaagccctat gtgcgggcga ctgcggcttt tatccatcgc cgttttggcc tataatcatg
      361 gtccgacacg accaggcata cggaggcgcc ttttgaaaag catattccgt gtgtggcggc
      421 tgggtgtact ggttctgacc ttcctggggt ttggggctat gtccgttgcc gctcgcgcag
      481 acgaaatacc cgtgcccagc ctgtggtata gcccactgga gatccatttc ggttccgtgc
      541 ccatcgacgg ccgggtcgag cgccaggtcg acgtccataa cctgggcacg gcgccgatga
      601 ccctcagcgg cggcgaggtc gaaaagccct tcagcatcga ctatgacacc tgcggcggcg
      661 tggtggccgc cggggcgtcg tgccggctca ccttctcgtt cgcgccaacg gccgcgggcg
      721 acttcaccgc cgaggccaat ggcgacagcg acgccgggcc gtggcacgtg gtcttgcgcg
      781 gccgcaccgc cgcgccggag atcaccgtca gcccgcgctc gctggacttt ggccgaggcc
      841 tggttggcag cgccttccca cctcaggtgg tcgtcgtcac caacattggc ggcgtgcccg
      901 tgggcggctt cagggccgaa ccggtcagcg tgcccttcac cggcgggctg ggccagtgcg
      961 cggcggggct gcaacccggg cagtcgtgcc acatgacttt cgacttcgcc gccgagaacg
     1021 ccggcgtctt ccacaagttc tggaccgccc agagtgacgc cggccccatc gagatcgagc
     1081 tacagggccg cacgtatacc ggcatcgccg gcacaggcca gggcgtcacg ccgcgggcca
     1141 ttgacttcgg cccggtgcgc gtcggcgaca cgatccggca gacggtcaca ttccacaact
     1201 tcgacccctc gatccccatc gtcaattggg actactcctg gatcaccgac gacccctaca
     1261 tcgacttcga ctatcagacg aactgtggtg aatcgctgcc cgggctggcc gagtgcaaag
     1321 tgaccatcag ctaccggccg cgcgacttcg gcgacgacac cgttatcctc aacgtgctca
     1381 acagccaggg catcattgat atcaacctct ggggccaggg cgcggcggcc gagatcgtcg
     1441 ccgattcgcc ggccatcgat ctgggcatgg ccacagacgg ccgcggcagc gaccaggtgg
     1501 tgcgcttcac caacctcggc cacggcccgg ccgacgtgct gggcatcgag agcgcgccgt
     1561 cgttcatcat cagcgacagc gattgcggcg acgtgctggc ccccggccag agctgccggg
     1621 cgtgggtgcg tttcgacccc aacggctatg gccgccgcga agaggaaatc gccctgctga
     1681 ccggggccga ccccgtgcct gtgcgcgtcg ttggcgggct gctgacgccg caactggctg
     1741 tcgccttcgc cccgcgcgcg gccacagtgg gcgacctcgt ccatctgcgc ctgaccttca
     1801 gcaacgccaa cccggcccag gcgctgttcg acctgggcct ggacggacaa ctgcccggtg
     1861 gcctggctct gctcgacggg ccgccggagg cctcggccaa ctgtggcggg ccgacgctga
     1921 cccgcgcggc caacggcaca gagttctctc tgcgtgatat gaccctggcc gctggcgata
     1981 cgtgcatcgt tgacctggcg gcgcaggcgg tgctggccgg ccagtggcgc ttcgattcgg
     2041 ccgcctatag ccacgccgga cctagccaat tgagcggcga tagcctggtg gtcaccgacg
     2101 acgaggagac gttcacctat cagctattcc tgccctctct gctaggcccg accaactgag
     2161 ccgccggaga acgacaatgc acattgacgt cttttccgac accgtctgtc cctggtgtcg
     2221 catcggcaag cggcatctgg agttggcgct ggcgggctgg ggcgggtcgc cggccgagcc
     2281 ggtcaccgtc cgctatcgca gcttcttcct cgacccgacc attccgccag aggggcgcga
     2341 cttccgcgac cacatgctgg ccaagggcgg cgggcgggtg gccctggagg actttttcgc
     2401 cgcgccgcgc gagcggggcg cggccgtcgg cctgagcttc aacttcgagg ccatccagcg
     2461 cgctcccaat acgctgcttt cccaccggct ggtctacctc gcgccagagg cgcggcgcgg
     2521 cccagtgctc gacgccctct atgaggccta cttcgcccgc ggcgaggata ttggcgacgt
     2581 ggcgacgctg gcggtggtgg ccgagcgcgc cgggctggac gggccaacgg tggtggccca
     2641 actggccacc accgccggaa cggccgaagt ctgggccgat gtggactatg cccggcgggc
     2701 cggcatccgc ggcgtgccct tcttcatctt caacgagcgc ctggccttca gcggcgcgca
     2761 gccgccagag gtgattcagc gcgtgctgca ccaggccgcg gccgaagcaa gtcaggccca
     2821 gccgcgcgac cagggcaaat tgacccaata atgagcactg aatagcctgt cgccacctgc
     2881 tcgtcctgag tatgctgtac tcatcctgta cgatggctgg aggctagatg agtacgcgac
     2941 gagtggcaca agcggcaatg ggggtgggtg cgctggtggt ggcgttgttc ctggtgagcg
     3001 cctgggcgcg gccggcggcg gccgtgggcg ggggcagcgg gcgctggttg cagatgatgt
     3061 ccaacgatgg tggcgttggc ccaaacgaca tcgctgggcc ggacgatgac ccggtggtgg
     3121 ccttcacgcg gcccggcagc gtcagtcccg gctgcgacgc gccaactgtc cattacggtg
     3181 ttggcgacgc ggtgctctac ttcgcccgcc atcgctgttg gctgccctgg ttcgacggcg
     3241 aaggttccgg cctgcccgcc ggcgcggaca tcaatgctct tcacgacgaa tgcggcccga
     3301 ccgagccgcg ctgcaccatc tatctgagct tcaccaggac gctgcgcgtg cccggcgtgg
     3361 gcaaggtgca gccccatgat atcgttagcg cggactgggt tgagggcacc caggatatct
     3421 ataccaattt ctggctggtc ttcgatggct ccgatgtcgg cctgaccgag ccgggcgagc
     3481 gcattgacgc tctattcctg ttcgacgagg acaggccaga accctaccgg gactgtgtcg
     3541 ccttgggctg ggccagcacg gtgggcaact accgcgtgcg cgaccagtgg ggcggctggc
     3601 tgaccggcgg cggcgaggac attctgggct tttgcgccag cagcatcggc gaggacacca
     3661 gcggctactg gttcctctac cacgacggct cggccgaagg cttgccgcgc aatgctctcg
     3721 tcggcctggc ccacgaagag ggacgcgctg cctccgggcg gttcaacttc ctgaccaaag
     3781 gccccttcca ggccgacgag gccgacggcg gcgcgggcga catcttcgag ttcagcagct
     3841 acaacggcgg catgtaccgc ggcccgctgg tgagtttctg ggacgaccta ggggttgcgg
     3901 gtgttcccga tagcatccac atctactaca cacacgagta gacctgagca cgagaacgcg
     3961 gatcgcgtag cgacgcgcac cggtcgccgt cgcgtctcta ccgatctgcg tccatccccg
     4021 ttcgatgaca acaaagagcg ccggacagaa ggcggacttc cctctcccgg cgctctccgc
     4081 gcccgcggcc ggcgcggcgg ccg
//
LOCUS       DAOSMQ010000286         4084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22007_length_4084_cov_4.445768, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000286 DAOSMQ010000000
VERSION     DAOSMQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22007_length_4084_cov_4.445768"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1683)
                     /locus_tag="PK829_09895"
     CDS             complement(61..1683)
                     /locus_tag="PK829_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU41568.1"
                     /translation="MDPRSDCIDFINPATGEKFGEVRMTTEEEVMLARREMAAAQSTW
                     AAKPLRERARIVKQFQRVVIDAADELTAIVNQDHGKSRGDALHEIVMTVDKIHQYARL
                     SPKWLGRRRIPPGLYFFRRYYTERVPYGVVGIIGPWNYPIDLMLPPIIAALLAGNTVV
                     VKPSEVAAATGVKLEELFGRVPELAPFVRFVHGDGRVGAALVRSQPDLLFLTGSVKTG
                     QIVARAAAENMTPFLYELGGKDPMLVLEDADIQAAAHWGVWGAFYTTGQACVSVERVY
                     VHHKVYDAFVAAVLEETAQFSVGYSADIDNPYRMGPLTFQRQCDIVNTHLHDAIDKGA
                     RVLMGGRQTGMFIEPTVLVDVDHTMKIMTEETFGPVLPIMKVFDDTHAIQMANHSDMG
                     LSAYIWSRDLNHAKRVAEQVQVGVVNINDTMSHYPVSLLPFGGVKMSGNARTHGAGEV
                     TQFTHTKSYAVGAPPHPLDPATMVRYPGHYRLGKAILGLFFGVTPQQRVEPVLDYLRE
                     DPTGQVVTRAAAGVGAASLVGLAVAALIRGKK"
     gene            complement(1708..3555)
                     /locus_tag="PK829_09900"
     CDS             complement(1708..3555)
                     /locus_tag="PK829_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOU41569.1"
                     /translation="MSVQLAGRETNGAATMPDPVYDYVIIGSGFGGSVSAWRLTEKGY
                     SVLVIERGRRWSAETFPKTNWNVFDYLWMPALRCFGPQGLNFFKDIWLLNGSGVGGGS
                     LIYASTHRIPPRRFFEAAEWGELADWEAELMPHYHTANRMLGTATNPCLWPADHTLKA
                     IADDLGQGDTFEATGVGIYFGERGVTVSDPYFDGDGPTRTGCLHCGGCMVGCRHNAKN
                     TLDKNYLYLAERYGAEVLPERNVRVIRPLYGAQADGARYQVEYEKITDWGRKRRGAAR
                     ARNVIVAAGVVGTVELLLKCRDEDRTLPRLSPMLGEHVRSNSEALMGVTARGREQDFA
                     EGVAISSHFWADEVTSVEPCRYPPGSGFMRTLIWPLEGYGNEGTTFAGRLWASLQHLF
                     RKPGDFLHTRLLPHWAERDTVLLIMQTVENKMAFRRGRTLSVGGRKGVYTERDPEDPI
                     PACVDAGSDVVRRFAEKADGVPWVGLNDLLGIPNTAHILGGCPIGADEQRGVVDVHQQ
                     AFNYPGLYVADGSVVPTNLGVNPSLTIAAMTERAMSFIPARAEAPAVAPLPYPHGVTV
                     DGKAIATELAANGNGNGEVPAWRRYAPLLALLLPLLPVLLALFAFMRKK"
     gene            3790..>4084
                     /locus_tag="PK829_09905"
     CDS             3790..>4084
                     /locus_tag="PK829_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41570.1"
                     /translation="MTSFTLPPGHHHDTLDSTSPPVVLGRACPITGERLRTGDAVIVC
                     DARPGSDPIRAEGWATSTSCPHCGAPTGYATYVPPVWGATPPPAAPPARPPRPA"
BASE COUNT          650 a         1410 c         1347 g          677 t
ORIGIN      
        1 cccaccccgg cacacaggtg gattacctgg atctggtggc caacgccccg gcccccgggg
       61 ttactttttg ccgcgaatca gcgccgccac ggccaacccg accagcgacg ccgcgcccac
      121 ccccgcggcc gcccgtgtca cgacctgtcc ggtgggatct tcccgcagat agtctaacac
      181 tggctcaacg cgctgctgcg gcgtgacgcc gaagaacagc cccaggatgg ccttgcccag
      241 ccggtagtgg ccgggatagc gcaccatcgt cgccgggtcc agcgggtgcg gcggcgcgcc
      301 gacggcgtag cttttggtat gagtgaactg cgtcacctcg cccgcgccgt gggtgcgcgc
      361 gttgcccgac atcttcacgc cgccgaacgg cagcagcgac accgggtagt gcgacatggt
      421 gtcgttgatg ttgaccacgc ccacctgcac ctgctcggcc acgcgcttgg cgtggttcag
      481 gtcgcggctc cagatgtagg cgctcagacc catgtcactg tggttggcca tctggatggc
      541 gtgggtgtcg tcgaagactt tcatgatcgg cagcaccggg ccaaaggtct cctcggtcat
      601 gatcttcatg gtgtgatcga cgtcgaccag caccgtcggc tcgatgaaca tgcccgtctg
      661 ccggccgccc atgagcaccc gcgcgccttt gtcgatggcg tcgtggagat gggtgttgac
      721 gatgtcacac tggcgctgga aggtcagcgg ccccatgcgg taggggttgt cgatgtcggc
      781 tgagtagccg acgctgaact gggctgtctc ctccagcacc gcggccacga aggcatcgta
      841 gaccttgtgg tggacgtaga cgcgctcaac cgacacgcaa gcctgcccgg tggtgtaaaa
      901 cgcgccccag acgccccaat gggccgcggc ctggatatcg gcgtcctcca gaacgagcat
      961 cgggtccttg ccgcccagct catagaggaa gggagtcatg ttctcggccg ccgccctggc
     1021 cacgatctgg cccgtcttca ccgagccggt caggaagagc aggtcgggct ggctgcgcac
     1081 cagcgccgcg ccgacgcggc cgtcgccgtg gacgaagcgc acgaacgggg ccagttccgg
     1141 cacgcggccg aacagttcct ccagcttcac ccccgttgcc gcggccactt ccgacggctt
     1201 gaccacgaca gtgttgcccg ccagcagcgc ggcaatgatc ggcggcagca tcaggtcgat
     1261 ggggtagttc cacggcccaa tgatgccgac gacgccatag ggcacacgct cggtgtagta
     1321 gcggcggaaa aagtagaggc ccggcgggat gcgcctgcgc cccagccact tgggcgagag
     1381 gcgagcgtac tggtggattt tgtccaccgt catgacgatc tcgtgcaggg cgtcgccgcg
     1441 gctcttgccg tggtcctggt tgacaatggc cgtcagttcg tcggcggcgt cgatgaccac
     1501 gcgctggaac tgcttgacga tgcgcgcgcg ctcccttagc ggcttggccg cccaggtaga
     1561 ctgcgccgcg gccatctcgc ggcgggccag catgacctct tcctcggtcg tcatgcgcac
     1621 ttcgccgaac ttctcgcccg tcgccgggtt gataaagtcg atgcaatcgc ttctcgggtc
     1681 catcgttgcc tcgtaatgag tctcagccta cttcttgcgc atgaacgcga acagcgccag
     1741 caacacgggc aacagcggca gcaagagggc cagcagcggc gcgtagcgac gccaggcggg
     1801 aacctcgcca ttgccgttgc cgttggcggc cagctcggtg gcaatggctt tgccgtctac
     1861 agtcacgcca tgcggatagg gcagcggggc cacggccggc gcttcggccc gcgcgggaat
     1921 gaagctcatg gcccgctcgg tcatggcggc gatggtcagg ctggggttaa cacccaggtt
     1981 cgtcggcacg acggagccat cggccacgta gaggccggga tagttaaatg cctgctggtg
     2041 gacatcgacc acgccgcgct gctcgtcggc cccgatgggg cagccgccga ggatgtgggc
     2101 tgtgttgggg atgcccagca ggtcgttcag ccccacccag ggcacgccgt ccgctttttc
     2161 ggcaaagcgc cgcaccacgt cgctgccggc gtccacacag gccgggatcg ggtcctccgg
     2221 gtcgcgctcg gtgtagacgc ccttgcgccc gccgaccgac agcgtgcgcc cgcgccggaa
     2281 ggccatcttg ttctccaccg tctgcatgat cagcagcacc gtgtcgcgct cggcccagtg
     2341 gggcagcagg cgcgtgtgca ggaagtcgcc cggcttgcgg aagagatgtt gcagcgaagc
     2401 ccacagccgc ccggcgaacg tcgtcccctc gttgccgtag ccctccaggg gccagatgag
     2461 cgtgcgcatg aagcccgatc cgggcggata gcggcacggc tcgacgctgg tcacttcgtc
     2521 ggcccagaag tgggagctga tggccacgcc ctcggcgaag tcctgctcgc ggccgcgggc
     2581 ggtcacgccc atgagcgcct cgctgttgct gcgcacgtgc tcgcccaaca tcggcgacag
     2641 gcgcggcagc gtccggtctt cgtcgcggca cttcagcagc agttccaccg tgccgacgac
     2701 gccggcggcg acgatgacgt tgcgcgcccg cgccgcgccg cgccgcttgc ggccccaatc
     2761 ggtgatcttc tcatactcca cctggtagcg cgccccgtcg gcctgtgccc catagagcgg
     2821 ccggatgacg cggacgttgc gctctggcag cacctcggcc ccatagcgct cggccaggta
     2881 gaggtagttc ttgtccagcg tgttcttggc gttgtggcgg caaccgacca tgcagccgcc
     2941 gcagtggagg cagccggtac gcgtggggcc gtcaccgtcg aagtaggggt cggacacggt
     3001 gacgccgcgc tcgccaaagt agatgcccac gcccgtggcc tcaaaggtgt cgccctgacc
     3061 caggtcgtcg gcgatagcct tgagggtgtg gtcggccggc cagaggcagg ggttggtggc
     3121 cgtgcctaac atgcggttgg cggtgtggta gtggggcatc aactccgctt cccagtcggc
     3181 cagctcgccc cactcggccg cctcgaagaa gcggcgcggt gggatgcggt gggtgctggc
     3241 gtagatgagc gagccgccgc ccacgccaga gccgttgagc agccagatgt ccttgaagaa
     3301 gttcaacccc tgtggcccaa agcagcgcag cgccggcatc cacaggtagt cgaagacatt
     3361 ccagttcgtc ttggggaagg tttcggccga ccagcgcctg ccgcgctcga tgaccagcac
     3421 cgagtagccc ttctccgtca atcgccacgc ggaaacgctg ccgccgaatc cggagccaat
     3481 gatcacgtag tcatagacag ggtccggcat cgtcgccgcg ccgttggttt ccctaccggc
     3541 cagttgaacg ctcatgttgc gcttccttcg cccccggtgg cagccgccgg gcgcagacca
     3601 caggacatcg cgcgacggaa agccgcgtcg tgagaatagt ataacacatt gcgtcataga
     3661 aatatacaag gagtggcccg accaaagacc tgacaggtgc gcggcccgtt tggtcgtcca
     3721 cctgtcaggt tcctggcccc tcctcgactg gcccccggcc actaaattga gtattatcgg
     3781 cgcaatccca tgaccagctt cacccttcca cccggccacc accacgatac ccttgactcg
     3841 acctcgccgc cggtcgtgct gggtcgcgcc tgccccatca ccggcgagcg gctgcggacg
     3901 ggcgacgcgg tgatcgtctg cgacgcgcgg ccaggcagcg accccatccg cgccgagggc
     3961 tgggccacct caaccagttg cccccactgt ggcgcgccca ccggctacgc tacctacgtg
     4021 ccgcccgtct ggggcgcgac accgccaccg gccgcgccgc cggcccggcc gccgcgcccg
     4081 gccg
//
LOCUS       DAOSMQ010000287         4082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22023_length_4082_cov_4.882543, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000287 DAOSMQ010000000
VERSION     DAOSMQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22023_length_4082_cov_4.882543"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PK829_09910"
     CDS             <1..603
                     /locus_tag="PK829_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOU41571.1"
                     /translation="LNMQCDIYHPFQCLADLMTIMEKKGRDLRRRKVVVSWAYASSYL
                     KPISVPQSLVLQLPRFGMDVVLAHPPEFKLMPEIMAQAQEQARKYKTGFEVISDANGM
                     TEAVKDADVVYAKSWGPLLVTDDQTEGKRIQDQYKHWITNNELMSLAKEDAVYMHPLP
                     ADRDIEVTSEVMDGPNSVVFDQAENRLHAQKAVMALTMRS"
     gene            681..1628
                     /gene="arcC"
                     /locus_tag="PK829_09915"
     CDS             681..1628
                     /gene="arcC"
                     /locus_tag="PK829_09915"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOU41572.1"
                     /translation="MTQKLAVIAIGGNSLIKDKKHQSVEDQYDAAYETAGHIAKMIQA
                     GWNVAVAHGNGPQVGFILRRSELSAHELHEVPLDVCGADTQGAIGYALQQSLQNHFLD
                     MGIDKPVVTLVTQTEVDANDPAFQNPTKPIGSFMGEEDAQRRIEVDGWDCTEDAGRGW
                     RRVVASPLPMRIVEEPSVRALIDAGHIVITVGGGGIPVVADDQGHLRGIAAVIDKDFA
                     SSLLATRLGADTFIISTAVEKVALNFGKPEQRWLDRVTLAEAKQYLAEGTHFAKGSMA
                     PKIQAIINFLEAGGHHAIVTDPANIARALAGETGTHFVA"
     gene            1645..2901
                     /gene="thrC"
                     /locus_tag="PK829_09920"
     CDS             1645..2901
                     /gene="thrC"
                     /locus_tag="PK829_09920"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU41573.1"
                     /translation="MDHVLHLRSLLSGHTFRPDEIDYVDPEFGPEGIVDVVYDYDLIG
                     RRISRESLRRDENHSIWRYRPLLPIEPDSPLPPLQIGWTPLYETPGLAASLGLRHLWV
                     KDDGRNPTASFKDRASAIAVVKARERGAKIVTTASTGNAAAALSGICASVGQANVIFV
                     PEKAPAAKVAQLLAYGSTVMLVRGTYDDAFELCLQAADAYGWYNRNTGFNPYMSEGKK
                     TATLEICEQLNWNAPDAIFVSVGDGCIIGGLHKGLKDLMALGWIERLPRLIGVQAAGS
                     NYLAEAWERGEDVFTKPPIQAETVADSISAGLPRDRLKAMAAVVETNGAYVTVSDDEI
                     LAAIPLLARGSGVFAEPAGAAAYAGLIKAVAQGLVSADERIVVLNTGSGLKDVVGAMK
                     GVELVKTQPYRVDPDLEDLRRVMATI"
     gene            complement(2957..3400)
                     /locus_tag="PK829_09925"
     CDS             complement(2957..3400)
                     /locus_tag="PK829_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41574.1"
                     /translation="MGRRIITPYTLEQTKGTDPAQSPVLRIDSYFDRVIKSIPGDVVA
                     GWTAILGLFGATPGLVAGVTTTNILWILWALLTIITFFWTYRQTQLQGVQPAWTQVFV
                     STFAFVVWVLALGAPFNTLSFYDTRLAAAVLIVFTLAVGAIQPHE"
     gene            complement(3519..>4082)
                     /locus_tag="PK829_09930"
     CDS             complement(3519..>4082)
                     /locus_tag="PK829_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOU41575.1"
                     /translation="LVRFDYHANADGVTTQGRVYEFQPDFYVGSKDTELDFALVKIKG
                     EPLAERKMQPGDEGLGYLELLRRGRHRGYLLLAPRMLVEHERVNIIQHPNGNPQKVVL
                     TQNYVLADMTADRVHYLADTQPGSSGSPVLNRYWEVVALHHSGGAHPPQKASNNLQKV
                     LKGLYKFNEGIPIRAILPKIERYLPTK"
BASE COUNT          752 a         1359 c         1314 g          657 t
ORIGIN      
        1 ctcaacatgc agtgcgacat ctatcacccc ttccagtgcc tggccgacct gatgaccatc
       61 atggagaaaa aggggcgtga cctgcggcgg cgcaaggtcg tcgtgtcgtg ggcctacgct
      121 tcgtcgtacc tgaagcccat ctccgtgccg caatcgctcg tgctgcaact gccgcgcttc
      181 ggcatggatg tcgtcctggc ccacccgccg gagttcaagc tcatgccgga gatcatggcc
      241 caggcccagg agcaggcgcg caagtacaag accggcttcg aggtcatcag cgacgccaac
      301 ggcatgaccg aggccgtcaa ggatgccgac gtcgtctacg ccaagtcgtg ggggccactg
      361 ctggtcaccg acgaccagac cgagggcaag cgcatccagg atcagtacaa gcactggatc
      421 accaacaacg agttgatgtc gctggccaag gaagacgccg tctacatgca ccccctgccg
      481 gccgaccgcg acatcgaggt caccagcgag gtcatggacg gccccaattc ggtcgtcttc
      541 gaccaggccg agaaccggct gcacgcgcag aaggccgtca tggccctgac aatgcgttcc
      601 tgagagaccg aaccacagat ggcccagttc cgactgtgtc atctgtggtt ccttttttat
      661 ttcccacaag agagaacccc atgacccaaa aactagccgt cattgccatc ggcggcaatt
      721 cgttgattaa ggacaagaag caccagtcag tggaagacca gtacgacgcg gcctatgaga
      781 cagccgggca cattgccaag atgatccagg ccggctggaa cgttgccgtg gcccacggca
      841 acggcccgca ggtcggcttt atcctgcgcc gcagtgagct gtcggcgcac gagttgcacg
      901 aggtcccgct cgacgtgtgc ggcgcggaca cgcaaggggc catcggctac gccctccagc
      961 agagcctcca gaaccacttc ctcgacatgg gcatcgacaa gccggtcgtc accctcgtca
     1021 cccagaccga agtggatgcc aacgaccccg ccttccagaa cccgaccaag ccgatcggct
     1081 cgttcatggg cgaagaagac gcccagcgcc gcattgaggt cgatggctgg gactgcaccg
     1141 aggacgccgg gcgcggctgg cggcgcgtgg tcgcctcgcc gctgcccatg cgcatcgtcg
     1201 aggagccgtc tgtccgggcg ctcatcgacg ccgggcacat cgtcatcact gtcggcggcg
     1261 gcggcatccc cgttgtggcc gacgaccagg gccacctgcg cggcatcgcc gcggtcatcg
     1321 acaaggactt cgcctcgtcg ctgctggcca cgcggctggg cgcggatacg ttcatcatca
     1381 gcacggccgt cgagaaggta gccctcaact tcggcaagcc ggagcagcgt tggctcgacc
     1441 gcgtgacgct ggccgaggcc aaacaatacc tggccgaagg cacccacttc gccaaaggca
     1501 gcatggcccc caagattcag gccatcatca acttcctgga agcgggcggc caccacgcca
     1561 tcgtcaccga cccggcgaac atcgcccgcg cgctggccgg ggaaaccggc acgcacttcg
     1621 tggcctagcc tgcgacttga caatatggat cacgtattac acctccgaag cctcctcagt
     1681 ggccacacct ttcggccgga cgagatcgac tacgttgacc cggagttcgg gccggaaggc
     1741 attgtcgatg tcgtctacga ctacgacctg atcggccggc gcatcagccg cgagagcctg
     1801 cgccgcgacg agaaccactc catctggcgc taccggccgc tgttgcccat cgagccggat
     1861 tcgcccctgc cgccgctgca aatcggctgg acgccactct acgagacgcc gggcctggcc
     1921 gcgtcgctgg gcctgcgcca cctctgggtg aaggacgacg gccgcaaccc gaccgcctcc
     1981 ttcaaggatc gcgccagcgc catcgccgtc gtcaaggccc gcgagcgcgg cgcgaagatc
     2041 gtcaccacgg ccagcaccgg caacgccgcc gccgccctca gcggcatctg cgccagcgtc
     2101 ggccaggcca acgtcatctt cgtaccggag aaagcgccgg cggccaaagt cgcccagctg
     2161 ctggcctacg ggtcaacggt gatgctggtg cgcggcacct atgacgacgc cttcgagctg
     2221 tgcctgcaag cggccgacgc ctacggctgg tacaaccgca acaccggctt caacccctac
     2281 atgagcgagg gcaagaagac ggccacgctg gagatctgcg agcaactcaa ttggaacgcg
     2341 cccgacgcca tcttcgtcag cgtcggcgac ggctgcatca tcggcgggct gcacaagggg
     2401 ctgaaagacc tcatggccct gggctggatc gagcggctgc cgcgcctcat cggcgtgcag
     2461 gccgcgggca gcaactacct ggccgaggcc tgggagcggg gcgaggacgt gttcaccaag
     2521 ccgcccatcc aggccgagac ggtggccgac agcatcagcg ccggcctgcc gcgcgaccgg
     2581 ctgaaggcca tggccgccgt cgtggaaacc aacggggcct acgttactgt gtcggacgat
     2641 gagattctgg ccgctattcc cctgctggcc cgtggcagcg gcgtcttcgc cgagccggct
     2701 ggcgcggccg cctacgccgg gctgatcaag gccgtggcgc aggggctggt gtcggccgac
     2761 gagcgtatcg tcgtgcttaa caccggcagc ggcctgaagg acgtggttgg cgcgatgaag
     2821 ggcgtcgagc tggtcaagac ccagccttac cgcgtcgatc ccgatctgga agacctgcgc
     2881 cgggtgatgg cgaccatctg acgggtaggg ctttgggtag gggcgggtct tagacccgcc
     2941 cctacagggc ttgcccctac tcgtgcggtt ggatggctcc gacagccagc gtaaagacga
     3001 tcagcaccgc cgccgccagg cgggtgtcat agaagctgag cgtattgaac ggcgcgccca
     3061 gcgccagcac ccacaccacg aaagcgaacg tcgagacaaa gacctgcgtc caggccggct
     3121 gcacgccctg aagctgcgtc tgccggtagg tccagaagaa ggtgatgatc gtcagcaacg
     3181 cccacaaaat ccacaggatg ttggtcgtcg tgacgccggc gaccaaaccg ggcgtggccc
     3241 cgaagaggcc gaggatggcc gtccagccgg cgaccacatc gccgggaatg ctcttgatga
     3301 cacggtcgaa gtagctatcg atgcgcaaga ctggcgactg ggccgggtcg gtgcctttgg
     3361 tttgttccag ggtgtacggg gtgatgatgc gacgtcccat gacttggttg tctcctgcgc
     3421 gatggggcct ggcgaccgga cggccgccag acccgcctaa cttggatcac tcattctgcc
     3481 ggctggatac tgctcgcggc caagtgacca cttgcgcctc acttggtggg caagtagcgc
     3541 tcgatcttcg gcaggatcgc ccggatggga ataccctcgt tgaacttgta gaggcccttc
     3601 aacacctttt gcaggttgtt gctggccttc tggggcgggt gggcgccgcc gctgtggtgc
     3661 agggcgacga cctcccagta gcgattgagc accggcgagc cggatgagcc gggctgcgtg
     3721 tcggccaggt agtgcacccg gtcggcggtc atgtcggcca ggacgtagtt ctgggtcagc
     3781 acaaccttct gcgggttgcc gttgggatgc tggatgatgt tgacccgctc gtgctcgacg
     3841 agcatgcgcg gggccagcag caagtagccg cggtgccggc cgcgccgcag cagctccaga
     3901 tagcccaggc cctcgtcgcc cggctgcatc ttgcgctcgg ccagcggctc gcccttgatc
     3961 ttgaccaggg caaagtcgag ttccgtgtcc ttgctgccga cgtagaagtc gggctggaac
     4021 tcatagacgc gcccctgggt ggtgacgccg tcggcgttgg cgtggtagtc gaagcgcacc
     4081 aa
//
LOCUS       DAOSMQ010000288         4061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22184_length_4061_cov_5.037194, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000288 DAOSMQ010000000
VERSION     DAOSMQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22184_length_4061_cov_5.037194"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2506
                     /locus_tag="PK829_09935"
     CDS             <1..2506
                     /locus_tag="PK829_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU41576.1"
                     /translation="PSGQPRRWDAASGNPAPYDFFRNVAGIYSAIQQYGVGKAVSGDL
                     LEVFPGTYVEQVTVNKTMTLLGHGPATTFIKAPATLPNTSTQDSIIVKITGAGVQVEM
                     SEFTVTGPGPSGCGSIGYGIFVRDGAYANIHDNKVVDIKDTGLSGCQNGNAIGVGRQL
                     WSTSGTADITDNEISTYQKTGVIVDNTGSSANITGNTITGDGPISYIAENGIQVSRGA
                     TAEINGNTITGHSYAPFTATSTGMLLYQGGTVNTDGNTVTQNQIGINVIDTSGTHKNN
                     IVTATAAGTGSPGFWGIVVDAPPPGRVPSPFDADGNAAAATGVGPNPPAAVQTVLVTG
                     NTFTSNNSAGGVGVETDAGYGSLDINVTVTKNLIHNWETGVVVYECTGGGCAASTFSN
                     VDVNRNSIVGNTDGFYADTVDPNETDGTCNWWGDASGPSGAGSGTGDTVSTEVDFTPW
                     LYSSDLDGPCYVGGTISIDKVAAGGGATQFTFDVSWSATNVTLTDASAPYVTNPPLQA
                     GNYTIAEINLPSGWSQQSATCDNTATTPVETVNPSSITVADGDAWVCTFTNVYTPPPA
                     NTCPLPSASSQYTDLLGGGMGNPKKHKVQLKITLPNSANLVDLYGQMVAKDTGQSKYV
                     RFIMPGKNNYVEVNTITAPIEHQFGNFWYGADVYDAGTPPAWLKGQWFLQKSGIKTHI
                     PRAFVVYATYQHPTNTYVNVWDTFTPAEGEVYWDTAQGWTPTRVINVSIPAPLGPTTF
                     NVELALVDNDKDARPVWVTVTAGNVSQTQKPTKASNGELLNLMTFTLTNVPAGTNQIV
                     ITVYSPSPTIDGFDGDSATLVGMAANYQCAPISAAP"
     gene            complement(2620..3141)
                     /locus_tag="PK829_09940"
     CDS             complement(2620..3141)
                     /locus_tag="PK829_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOU41577.1"
                     /translation="MATPPIDKTLFERLLQADELGIRLEIVGGLPMWEASPVLRHQEA
                     VDRIRSSIRRPAPQSGAAGRCDCYHAADVYVVFPDGSLKRPDIAVYCRRPEESDEAIT
                     LLPEAVIEIISKGYEAKDLEIGPRFYLSQGVKDVVVFDPHTLLVLHLTPAAVERHVSP
                     VDITLACGCACRV"
     gene            complement(3171..>4061)
                     /locus_tag="PK829_09945"
     CDS             complement(3171..>4061)
                     /locus_tag="PK829_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOU41578.1"
                     /translation="INDSPFAGREGKYVTSRQLRDRLMRELESNVALRVEDMERAGEF
                     LVSGRGELHLAILIETMRREGYEFAVGRPEVIFRESPEGRREPFEQVYVEALNDYWGG
                     VAEMLGKRRGQMLDIRYGEDGTVYAEYSVPTRGILGFRQPFLTATRGTGVFHALFDAY
                     LPYAGDIDHTEFGSLVSLESGPVSAYALEHLTARGTFFVRPSVDEVYGGQVVGEHIRD
                     EDLVINVCRTKNLTGHRAKPTAIIEALPSPRILSLDEAIEYLHEDELLEVTPASLRIR
                     KMELDHNLRMRAAKRVKYAE"
BASE COUNT          762 a         1436 c         1257 g          606 t
ORIGIN      
        1 ccccagcggc cagccgcgac gctgggacgc cgcctccggc aaccccgcgc cctacgactt
       61 cttccgcaac gtggccggca tctacagcgc catccagcag tatggcgtgg gcaaggcggt
      121 gtcgggcgac ttgctggaag tgttccccgg aacctacgtc gagcaggtga cggttaacaa
      181 gaccatgacc ctgctgggcc acggcccggc gacgaccttc atcaaggccc cggcgaccct
      241 gcccaacacc agcacgcagg actcgatcat cgtcaagatc accggcgcgg gtgtgcaggt
      301 ggagatgagt gagttcaccg tcaccggccc cggcccatcc ggctgcggca gcatcggcta
      361 cggcatcttc gtccgcgacg gcgcgtatgc caatatccac gacaacaaag tcgtcgatat
      421 caaggacacc ggcctcagcg gctgccagaa cggcaacgcc atcggcgtcg gccgccagct
      481 gtggagcacg agcggcacgg ccgacatcac cgacaacgag atcagcacct atcagaagac
      541 gggcgtcatc gttgacaaca ccggctccag cgccaacatc accggcaaca ccatcactgg
      601 cgacggcccg atcagctaca tcgccgagaa cggcatccag gtgtcgcgcg gggccacggc
      661 cgagatcaat ggcaacacca tcaccggcca ctcctacgcg ccgttcacgg ccacatcgac
      721 aggcatgctg ctctaccagg gcggcacggt caacaccgac ggcaacacgg tgacacagaa
      781 ccagatcggt atcaacgtga tcgatacctc cggcacgcac aagaacaaca tcgtcacggc
      841 cacggccgcc ggcaccggtt cgcctggctt ctggggcatc gtcgtcgacg cgccgccgcc
      901 gggccgcgtg ccctcgccgt tcgatgccga tgggaacgcc gccgcggcca ccggcgtcgg
      961 cccgaacccc cccgccgcgg tgcagaccgt tctggtgacg ggcaacacct tcaccagcaa
     1021 caactcggcc ggcggcgtgg gcgtggagac ggacgcaggc tatggcagcc tcgacatcaa
     1081 cgtgaccgtc accaagaacc tcatccacaa ctgggagacg ggcgtggtcg tctacgagtg
     1141 caccggcggc ggttgcgcgg cctcgacgtt cagcaacgtc gatgtcaatc gcaacagcat
     1201 cgttggcaac accgacggct tctacgccga cacggtcgat cccaatgaga ccgacggcac
     1261 gtgcaactgg tggggcgatg cctccggccc gtcgggcgca ggctccggca cgggcgacac
     1321 ggtcagcacg gaggtggact tcaccccctg gctctacagc agcgatctgg acggcccgtg
     1381 ctacgttggc gggacgatca gcatcgacaa ggtggccgcc ggcggcggcg cgacgcagtt
     1441 cacgttcgac gtgagctgga gcgcgaccaa cgtcactctg accgacgcca gcgcgcccta
     1501 cgtgaccaac ccgccgctgc aagccggcaa ctacaccatc gccgagatca acctgcccag
     1561 cggctggtcg cagcagagcg ccacgtgcga caacacggcg accacgccag tggagacggt
     1621 caatccgtcg tccatcaccg tggccgatgg cgacgcctgg gtgtgcacct tcaccaacgt
     1681 ctacaccccg ccgccggcca acacctgccc gctgccctcg gccagctcgc agtacaccga
     1741 cctgctgggc ggtggcatgg gcaaccccaa gaagcacaag gtgcaactga agatcaccct
     1801 gcccaactcc gccaacctgg tcgatctcta cggccagatg gtggccaagg acaccggtca
     1861 gtcgaagtac gtgcgcttca tcatgcccgg caagaataac tacgtcgaag tcaacaccat
     1921 taccgcgccg attgagcacc agttcggcaa cttctggtat ggcgctgatg tctatgacgc
     1981 gggcacaccg ccagcctggc tgaagggcca gtggttcctg cagaagagcg gcatcaagac
     2041 ccacattccg cgggccttcg tcgtctacgc cacctatcag caccccacca atacctatgt
     2101 gaacgtctgg gacaccttca ccccggccga gggcgaagtg tactgggata ccgcccaggg
     2161 ctggacgccc acccgcgtga tcaacgtgtc catccccgcg ccgctcggcc cgacgacgtt
     2221 taacgtcgaa ctggcccttg tggacaacga caaggacgcg cgcccggtgt gggtgacggt
     2281 gacagccggc aacgtctcgc agacgcagaa gccgaccaaa gccagcaacg gcgagctgct
     2341 taacctgatg accttcaccc tgaccaatgt gcccgcgggc accaaccaga tcgtcatcac
     2401 ggtctactcg ccgtcgccga ccatcgatgg cttcgacggc gactcggcca cgctggtggg
     2461 catggccgcc aactatcagt gcgcgccgat cagcgctgcg ccgtagttcc tccccgcgcc
     2521 ggttgatcga accggcgatg tagtgaagcc ccgcccggtc gcgaccgccc gggcggggct
     2581 ttttgttgtc atcgggccgg ccgccgcccg ttggcgcggc tagacccggc aggcgcagcc
     2641 acacgccagg gtgatgtcca ccggcgaaac gtggcgctcg acagccgccg gcgtcaggtg
     2701 gagcaccagc aacgtgtgcg gatcgaagac gaccacgtct ttgacaccct gagacaggta
     2761 gaagcgcggg ccgatctcca ggtctttggc ctcgtagccc ttgctgatga tctcgatcac
     2821 cgcctctggc aagagtgtga tggcctcatc tgactcttcc ggccgccggc aatagacggc
     2881 gatgtcgggc cgcttcaacg agccatcggg gaagaccacg tagacgtccg ccgcatgata
     2941 gcagtcgcag cgaccggccg cgcccgactg cggagccgga cgccggatgg acgaccggat
     3001 gcgatccacg gcctcctgat ggcgcagaac ggggctggcc tcccacatgg gcaggccgcc
     3061 gacgatctcc agacggatgc ccaactcgtc ggcctgcaac aggcgctcga agagggtctt
     3121 gtcgataggc ggcgtagcca tgagggcatt ataccacagc gcgagggccg ctattcggcg
     3181 tacttgaccc gcttggccgc ccgcatgcgc aggttgtggt ctagctccat cttgcggatg
     3241 cgcagcgacg ccggcgtgac ctcgagcagt tcgtcctcgt gcaggtactc gatggcctca
     3301 tcgagcgaca ggatgcgcgg cgacggcagc gcctcgatga tcgccgtcgg cttggcccgg
     3361 tggccggtca ggttctttgt ccggcagacg ttgatgacca ggtcttcgtc gcggatgtgc
     3421 tcgccgacga cctggccgcc atagacctcg tccaccgacg gccgcacaaa gaacgtccct
     3481 cgcgccgtca ggtgctccag cgcgtaggcg ctgaccggcc cgctctccag cgacaccagc
     3541 gagccgaact cggtgtggtc gatgtcgccg gcatagggca ggtaagcgtc gaacagggcg
     3601 tggaagacgc ccgtgccgcg cgtggccgtc aggaacggct gccggaagcc caaaatgccg
     3661 cgcgtgggca cgctgtactc ggcatagacg gtgccgtcct cgccatagcg gatgtcgagc
     3721 atctggccgc gccgcttgcc cagcatctcg gccacgccgc cccaatagtc gttgagcgcc
     3781 tcgacgtaga cctgctcgaa cggctcccgc cgcccctccg gcgactcgcg gaagatgacc
     3841 tccggccggc cgacggcgaa ctcgtagccc tcgcggcgca tcgtctcgat gaggatggcc
     3901 aggtgcagct cgccccggcc gctgaccagg aattcgcccg cgcgctccat gtcctcgacg
     3961 cgcagggcga cgttgctctc caactcacgc atcagccggt cgcgcaactg gcggctggtg
     4021 acgtacttgc cctcgcgccc ggcgaagggg ctgtcgttga t
//
LOCUS       DAOSMQ010000289         4048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22290_length_4048_cov_5.255197, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000289 DAOSMQ010000000
VERSION     DAOSMQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22290_length_4048_cov_5.255197"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PK829_09950"
     CDS             <1..257
                     /locus_tag="PK829_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU41579.1"
                     /translation="LRALASPLVVEVRGEGLLVGVELKTAVAPILAAARQRGLLIINA
                     GDTTLRIAPPLIVTRDHIDAAVAILGACLVEEEKRLTQRG"
     gene            437..982
                     /locus_tag="PK829_09955"
     CDS             437..982
                     /locus_tag="PK829_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018924568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOU41580.1"
                     /translation="MSLDYRLNTTTGPTARPTGPRLAALTPADWPAVRAIYEEGIATG
                     NATFETAAPEWPAWDAARLPCCRLAARRGRRVLGWAALSPVSSRAVYRGVAEVSIYVA
                     AAARGQGVGRLLMGGLVAASEEAGLWTLQAGIFPENAASLALHRAFGFRVVGRRERLG
                     QLHGVWRDALLLERRSNRAGV"
     gene            1099..>4048
                     /locus_tag="PK829_09960"
     CDS             1099..>4048
                     /locus_tag="PK829_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41581.1"
                     /translation="MTAIDADSSPPGGRRASSRRATVAWLLLILLLGLPLITPLLNGA
                     AVPCTHDGHLHYHRVAALAHAWGNGIHLTRWIPDLAFGYGYPFFVFREAAPLYAVLLP
                     HLAGLALPAASNLFYALTILAAGVFMGLWARDVLGARAAVVSAVAYMAAPYVLVDALV
                     RGNAPESLALPLLPFLLWVGRRWVLGGSVRAFLAGALGLALLSFSHNISTFIFAPTLL
                     VYLVALAVFGQQGSRGARERGSEEFSPAPLPPRSPTPFLRALALLALGLGLAFFYTGG
                     AVLEMDEVTLERSTTTRNNDWRFNFATAGEILAPVAPEDPTLVNPPLRLRLGWAPALL
                     AALGAAGLLWGRGADSRAREQRLHIGLMLAATAVYLFMALSISRPVWARLPLIDFVQF
                     PWRFVGRAALPVAFLAGVPWSDGRRLTADRRRLSFVGLVVAVAALLLEAVPNLYPRLC
                     AEEPFPTILTVHAYERATGLVGVDPEGSYFPRTVLERPAGSPLEADFAAGRTPQRFDA
                     TALPPGATLSDVVYAGQGVGLTVDAPTAFTARYLSFAFPGWSARVDGAPVAITAETPS
                     GLITFPVPAGSHRVEVRWGATPLRLALVGLSGLAGVGVVVVAVVGRRRRAARAAWATG
                     HAARLRWRAWAALALLALALLAVKALVIDRASTPLRAAAGPRLRAEEVTAIGSAEMRL
                     EGARPAVAQAVAGETFDIDMAWRLVAPATADYQTEVWLEDADGLVWSVKGTERPRVFE
                     DAPPTRQWAAGEWGWDSREVRVLSGTPPGDYAVVVTLFDRATLQPVTLSDSGSGVVLG
                     PTAVIGRATVVNPRRAQPPEAQYTRDAAVPDTGLRLLGYNQDRAAAAPGDAVLLTLFL
                     TCDGPGDCARVRLQLLDEAGAPRGAWDVPAIRAGLPAENWPAGGFLRGQHLLSLPRDL
                     ATGRYRFTLNGLPLGEISVTAPDRQFTAPPLAIELNAPFTAPAPVATLLGIATSPQPP
                     CTPPPPLFCLP"
BASE COUNT          470 a         1491 c         1459 g          628 t
ORIGIN      
        1 ggttgcgcgc cctggccagc ccgctggtcg tcgaggtgcg cggcgagggg ctgctggtcg
       61 gcgtggaact gaagacggcc gttgctccca ttctcgccgc ggcccggcag cgcggcctgc
      121 tgatcatcaa tgccggcgac accaccctgc gcatcgcccc gccgctcatc gtcacccgcg
      181 accacatcga cgccgccgtc gccatcctgg gcgcgtgcct ggtggaggaa gagaagaggt
      241 taacgcaaag aggttaacgc aaggacgcta agaagcaggg acgcaaagtc cttttcttct
      301 tgcgtcttgg cgtccgttgc gccctggcag aggttaacgc aaggacgcta agaagcaggg
      361 acgcaaagtc cttttcttct tgcgtcttgg cgtccgttgc gcctttgcgt tgaaattctt
      421 tacaggagaa gaagccatga gcctggatta ccgcctgaac accacgaccg gcccgaccgc
      481 ccgccccacc ggcccgcgcc tggcagcgct gacgccagcc gactggccgg ccgtgcgcgc
      541 catctacgag gaaggcatcg ccacgggcaa tgccaccttc gagacagccg cgccggagtg
      601 gccggcgtgg gacgcggccc ggctgccgtg ttgccggttg gcggcgcggc gcgggcggcg
      661 ggtgctgggc tgggcggcgc tcagtccggt gtcgtcgcgg gcggtctaca ggggcgtggc
      721 cgaggtcagc atctatgtcg ccgcggcggc gcgggggcag ggcgtcggcc ggctgctgat
      781 gggcgggctg gtggccgcgt cggaggaggc cggcctgtgg acgctgcaag cgggcatctt
      841 cccggagaac gccgccagcc tggcgctgca ccgcgccttc ggcttccgcg tcgtcggccg
      901 gcgcgagcgg ctggggcaac tccacggcgt ctggcgcgac gcgctgctac tggagcggcg
      961 cagcaatcgc gccggcgtct agggtgtgca tgccacggaa tgctggtcac gcccgccccg
     1021 agatagggac tagggactag cccccaacct gccgcgcggc gttgcccgtt cgccgcgcat
     1081 gggccatact tcgcggttgt gaccgccatc gacgccgaca gcagcccgcc cggcggccgg
     1141 cgcgcttctt cccgccgcgc cacagtcgcc tggctgctgc tcatcctcct gctcggcctg
     1201 ccgctgatca cgccgctgct caatggcgcg gccgtgccct gcacccacga cggccatctc
     1261 cactaccacc gcgtcgccgc cctggcccat gcgtggggca acggcatcca cctgacgcgc
     1321 tggatacccg atctggcctt cggctacggc tatcccttct tcgtcttccg cgaggccgcg
     1381 ccgctctacg ccgtgctgct gccccatctg gccgggctgg ccctgccggc ggcctcgaac
     1441 ctgttctatg ccctgaccat cctggccgcg ggcgtgttca tgggcctgtg ggcgcgcgac
     1501 gtattggggg cacgggcggc ggtcgtctcg gccgtggcct acatggccgc gccctacgtg
     1561 ctggtcgacg ccctcgtgcg cggcaacgcg ccggagtcgc tggccctgcc gctgttgccg
     1621 tttctgctgt gggtcgggcg gcgctgggtg ctgggcggct ccgtccgcgc cttcctggcc
     1681 ggggcgctcg gcctggcgct gctgtcgttt agccacaaca tctccacgtt catcttcgcg
     1741 ccgacgctgt tggtgtactt ggtagcattg gccgtctttg gacagcaggg gagcagggga
     1801 gcgagggagc gggggagcga agagttctcc cctgctcccc tgcccccccg ctcccccacc
     1861 cccttcctcc gcgccctcgc cctgctcgcc ctcggcctcg gcctggcctt cttctacacc
     1921 ggcggggccg tgctggaaat ggacgaggtg acgctggagc gctcgacgac gacgcgcaac
     1981 aacgactggc gcttcaactt tgccaccgcc ggcgaaatcc tcgcccccgt cgccccggaa
     2041 gacccgacgc tggtcaaccc gccgctgcgg ttgcggctgg gctgggcgcc ggcgctgctg
     2101 gccgcgctgg gggcggccgg cctgctgtgg gggcgcggcg cggacagccg cgcccgcgag
     2161 cagcggctcc acatcgggct gatgctggcg gcgacggctg tctacctgtt tatggccctg
     2221 tccatctccc ggccggtgtg ggcgcggctg ccgctgatcg acttcgtgca attcccgtgg
     2281 cgcttcgtcg gccgagcggc gctgccggtg gccttcctgg ccggggtgcc gtggagtgac
     2341 ggccgacggc tgaccgccga ccgccggcgt ctatcgtttg ttggtctcgt cgtcgccgtc
     2401 gccgcgctgc tcctcgaagc cgtgcccaac ctctacccgc gcctgtgcgc cgaggagccg
     2461 ttcccgacca tcctgaccgt ccacgcctat gagcgggcca ccggcctggt cggcgtagac
     2521 ccggagggca gctacttccc gcgcacagtg ctggagcggc cggccggctc gccgctggag
     2581 gccgacttcg ccgccgggcg cacgccccag cgcttcgacg ccaccgcgct gccgcccgga
     2641 gcgacgctca gcgacgtggt ttacgccggg cagggcgtgg ggctgaccgt ggacgcgcca
     2701 acggccttca ccgcccgcta tctgagcttc gccttccccg gctggtcggc ccgcgtcgat
     2761 ggcgcgccgg tggccattac cgccgagacg ccgtcggggc tgatcacctt ccccgtgccg
     2821 gccggaagcc accgcgtcga ggtgcgctgg ggggcgacgc cgctgcggct ggcgctggtg
     2881 gggctgagcg ggctggccgg ggtgggcgtg gtcgtcgtgg ccgtagtagg ccggcgcagg
     2941 cgggcggctc gcgccgcgtg ggcgacgggc cacgcagcgc gactgcgttg gcgtgcgtgg
     3001 gcggcgctgg cgctgctggc cctggcgctg ctggccgtca aggcgctggt catcgaccgg
     3061 gccagcacgc ccctgagagc cgcggccggg ccacggctgc gggcggagga agtgaccgcg
     3121 attggctcgg ccgagatgcg tctggaaggg gcgcggccgg ccgtggccca ggcggtcgcg
     3181 ggcgagacgt tcgacatcga catggcctgg cggctggtcg ccccggccac ggccgactat
     3241 cagaccgagg tctggctgga ggacgcggac ggactggtct ggagtgtcaa gggcacggaa
     3301 cggccgcgcg tcttcgagga cgccccgccg acgcggcagt gggccgccgg cgaatggggc
     3361 tgggacagcc gcgaggtgcg cgtgctgagc ggcacgccgc cgggcgacta tgccgtggtc
     3421 gtgaccctgt tcgacagggc cacgctccag ccagtgacgt tgagcgatag cggcagtggc
     3481 gtggtgctcg gcccaacggc ggtcattggc cgggcgacgg tggtcaaccc gcgccgggcg
     3541 cagccgccgg aggcgcaata cacgcgggac gccgccgtgc cggacacggg gctgcgcctg
     3601 ctgggctaca accaggaccg ggcggcggcc gcgcccggcg atgccgtcct gctgacgctg
     3661 ttcctgacct gcgacgggcc gggcgactgc gcgcgcgtcc ggctgcaact gctggacgag
     3721 gccggcgcgc cgcgcggcgc gtgggacgtg cccgccatcc gcgcggggtt gccggccgag
     3781 aactggccgg ccggcggctt cctgcgcggc cagcacctgc tcagcctgcc gcgcgacctg
     3841 gccaccgggc gctatcgctt cacgctcaac ggcctgcccc tgggcgagat cagcgtgacc
     3901 gcgcccgacc gccaattcac cgccccgccc ctggccatcg aactcaacgc ccccttcacc
     3961 gcccccgccc ccgtcgccac gttgctggga atcgccacgt ctccccagcc cccctgcacc
     4021 ccccctcccc ccctcttctg cctccccc
//
LOCUS       DAOSMQ010000290         4043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22324_length_4043_cov_4.958124, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000290 DAOSMQ010000000
VERSION     DAOSMQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22324_length_4043_cov_4.958124"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PK829_09965"
     CDS             <1..1343
                     /locus_tag="PK829_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU41582.1"
                     /translation="GRIRGFVPISHLSDVTPGMGERRRQQRLARLRGEMIGVKIIEVD
                     RQRHRLVMSQREAQKEWEEKKRGELMETLKPGDVRSGRVSGMRDFGAFVDLGGADGLV
                     HISELSWHRIDHPREVVKLGDEIEVYVLGVDQESGRISLSRKKLLPNPWDTVAQRYAQ
                     NQLIEGKVTRILDYGAFAEIEPGVEGLLHVSQLSRNPVEDPRSVVKEGEVHLLRIVSI
                     DQKRQRIGLSLKAVTPTEQIEWMAQKELAEALRREEEEVAAAARVPARVEEQPAETET
                     PAVEETMTEAAPAGVEAEAEAPAMTPAAVETGVPEAAPAEDVAPADVAPADVAMTQTS
                     VEEAPTSVEETEVVAEAEETEAMAETETEEAVAEMADDAPTADAPTAEETLEAADEAV
                     DRLMERIDDVAAEAAEMEAGEEPAAGSQAQAEDDETTGQATPLQDADEAETETV"
     gene            1380..1625
                     /gene="infA"
                     /locus_tag="PK829_09970"
     CDS             1380..1625
                     /gene="infA"
                     /locus_tag="PK829_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU41583.1"
                     /translation="MANKEDKLQVEGTVIELLPNTQFMVELDNGHKVLSYLSGRMRKH
                     YIRILLGDRVQIEMTPYDLERGRITYRYRRNVPTAEA"
     gene            complement(1678..2607)
                     /gene="prmA"
                     /locus_tag="PK829_09975"
     CDS             complement(1678..2607)
                     /gene="prmA"
                     /locus_tag="PK829_09975"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOU41584.1"
                     /translation="MYWLEICIQTDGEGAEAVAEVLRPFAYQESVVLEQRGDETNLDP
                     NALAPEVTVKIYVPEDDDSPQLRRRVAEALYHLSRLYPLPEPTFRELQDADWANAWKE
                     HYRPFRLGRRLRIRPTWLAHEPDDDARPDDVTLVLDPGMAFGTGLHPTTQSCLRALED
                     LVEPGVTVLDAGTGSGILAIAAIRLGASAVTAFDTDALAVRATLDNAAQNGVVERLRV
                     WRGELDSVRAEAGPGPWDIVVANILAPVIIGLLGERGLLEYVAPDGHLILSGIIDEQG
                     PDVEAALSAAGGQVRRVITAGDWVTYVAGHRQG"
     gene            complement(2614..3774)
                     /gene="dnaJ"
                     /locus_tag="PK829_09980"
     CDS             complement(2614..3774)
                     /gene="dnaJ"
                     /locus_tag="PK829_09980"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU41585.1"
                     /translation="MAVKRDYYEVLEVSRTVTKDELKKQYRRLARQYHPDVNPEPEAA
                     ERFKEISEAYEVLSDDDKRSAYDRFGHAGVGNGGYGGFEQGFGGFADIFEEFFGTFSG
                     AGAGRGRRRRGPRKGADLRYDLTITFEEAIFGVEREVEYRRPETCTTCGGNGAEPGTK
                     PIACTTCGGTGEVRRVQQSILGQFVNVATCATCGGTGELIPNVCHECNGRKLVERTVT
                     KKVKVPPGVDSETQIRLTGEGGAGLDGGPAGNLFVVLNVAPHEYFQRRGDDILLVLQI
                     NVSQAALGDEIAVPTVDGETTLAVPAGTQSGKEFRLRGLGVPKLDRSGRGANIGRGDQ
                     LVVLQVAIPKHLTPEQRELFQQLSRTLGKEVTPQKDRGILGQVKDALSDLFG"
     gene            complement(3847..>4043)
                     /locus_tag="PK829_09985"
     CDS             complement(3847..>4043)
                     /locus_tag="PK829_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41586.1"
                     /translation="KSAMGSGDPAGMTAAAERLQQALQSAGAAMYQNQGGQPDAESGS
                     GPANDRSDEDVVEGEFSDAN"
BASE COUNT          709 a         1350 c         1322 g          662 t
ORIGIN      
        1 acggccggat tcgcggcttt gtgcccatct cccacctcag cgacgtgacg cccggcatgg
       61 gcgagcgccg ccgccagcaa cgcctggccc gcctgcgcgg cgagatgatt ggcgtcaaga
      121 tcatcgaggt cgaccgccag cgccaccgcc tggtcatgtc gcagcgcgag gcgcaaaagg
      181 agtgggagga gaagaagcgc ggcgaactga tggagacgct caagccgggc gacgttcgta
      241 gcggccgcgt cagcggcatg cgcgacttcg gcgcgtttgt cgatttgggc ggggccgacg
      301 gtctggtaca catctccgaa ctgtcctggc accgcatcga tcatccgcgt gaggtcgtca
      361 agctgggcga cgagatcgag gtctacgtgc tgggcgtcga ccaggagtcg ggccgcatca
      421 gcctgagccg caagaaactg ctgcccaacc cgtgggacac cgtggcccag cgctacgcgc
      481 aaaatcaact catcgagggc aaggtcacgc gcattctcga ctatggcgcg ttcgccgaga
      541 tcgagccggg cgtcgagggt ttgctgcacg tctcgcagtt gtcgcgcaac cccgtcgagg
      601 acccgcgttc ggtggttaag gaaggcgaag tgcacctgct gcgcatcgtc agcatcgacc
      661 agaagcggca gcgcattggc ctgagcctga aggccgtgac gccgaccgag cagatcgagt
      721 ggatggccca gaaggaactg gccgaggcgt tgcggcgcga ggaagaagag gttgccgcgg
      781 ccgcccgcgt gcccgctcgc gtggaagagc agccggccga gaccgaaacc ccggccgtgg
      841 aagagacaat gacagaggcc gccccggccg gggtggaggc cgaagccgaa gcgccggcca
      901 tgaccccggc ggcggtggaa acgggcgttc cggaagcggc tccggccgaa gacgtggccc
      961 cggcagacgt ggccccggca gacgtggcca tgacccagac aagtgtcgaa gaggccccga
     1021 cgtcggtgga agagaccgag gtcgtggctg aagccgagga aactgaggcc atggccgaga
     1081 ccgagactga agaagccgtg gccgaaatgg ccgacgacgc cccgacggcc gacgccccga
     1141 cggccgagga gacgcttgag gccgccgatg aggctgtcga tcgtctgatg gaacgtattg
     1201 acgatgtcgc cgcagaggcg gctgagatgg aagcagggga agaaccggcg gccggctcgc
     1261 aggcgcaagc cgaggacgac gagaccaccg gccaggccac cccgctgcaa gacgccgacg
     1321 aggcggaaac cgagacagtc tagtttgagt ttattttctg ctttattttc aagggattta
     1381 tggctaataa ggaagacaag ctacaggtcg aaggcacggt tatcgaactt ctgcccaata
     1441 cgcagttcat ggtcgagttg gacaatggcc acaaggtgtt gtcctatctc tctgggcgga
     1501 tgcgcaagca ctacattcgc atcctcctgg gcgaccgtgt gcagatcgag atgactccct
     1561 atgacctgga acgcgggcgc attacctatc gctatcgtcg caacgtgccg acggccgaag
     1621 cctagcccgc cccagcccca ccgcgttctc ccgcgggcga gataaccgtc gggccggcta
     1681 tccttgccga tggccggcga cgtacgtcac ccaatcgccc gcggtgataa cccggcgcac
     1741 ctgccccccg gcagcgctta gcgccgcctc tacgtctggc ccctgctcat cgataatccc
     1801 gctcaggatt aggtgcccat ccggggctac atattccaag agtccgcgtt cgcccaacaa
     1861 gccgataatg accggggcca ggatgttggc cacaacgatg tcccacggcc ccggcccagc
     1921 ctcggcgcgg acgctgtcca gctcaccgcg ccagacgcgc agccgctcga cgacgccgtt
     1981 ctgcgcggcg ttgtccagcg tcgcccggac ggccagggca tcggtatcga aggcggtcac
     2041 ggccgaggcc cccagccgga tcgcggcaat agccaggata cccgaccccg tgccggcgtc
     2101 gagcacggtc acgcccggct ccaccaggtc ttccagcgcc cgcagacagc tctgtgttgt
     2161 tgggtgcaac cctgtgccaa aggccatgcc ggggtcgagc accagggtta cgtcgtcagg
     2221 tcgggcgtcg tcatccggct cgtgggccag ccaggtgggg cgaatgcgca ggcggcggcc
     2281 gagccggaac gggcggtagt gctctttcca ggcgttggcc caatcggcat cctgtaactc
     2341 gcggaacgtt ggctccggca gcgggtaaag gcggctcagg tgatagagcg cctcggccac
     2401 gcgccggcgc agctgcggcg aatcgtcgtc ttcaggcacg tagatcttga ccgtcacctc
     2461 cggcgccagc gcgttggggt cgagattggt ctcgtcgcca cgctgttcca ggacaacgct
     2521 ctcctgatag gcaaaaggcc gcagcacttc cgccacggcc tcggccccct cgccatcggt
     2581 ttgaatgcaa atctccagcc agtacatcgc cctctatcca aacaggtcgc tcagcgcgtc
     2641 cttaacctga cccaggatgc cccggtcttt ctgcggcgtc acctctttgc ccagcgtgcg
     2701 cgatagctgc tggaagagtt cccgctgctc cggcgtcagg tgcttgggaa tggcgacctg
     2761 cagaaccacc agttggtcgc cgcggccgat gttggccccg cggccgctgc gatccagctt
     2821 gggcacgccc aacccccgca gccggaactc tttgcccgac tgcgtgccgg ccggcactgc
     2881 cagcgtcgtc tcgccgtcca ccgtcggcac ggcgatctcg tcgcccagag ccgcctggga
     2941 gacattgatt tgcagcacga gaaggatgtc gtcgccgcga cgctggaagt actcatgcgg
     3001 agccacgttc agcacgacaa agaggttacc cgccggcccg ccgtcgaggc ccgcgccgcc
     3061 ctcgccggtc aggcgaatct gcgtctcgct gtctaccccc ggcggaacct tgaccttctt
     3121 ggtgacggta cgctccacca gtttgcggcc gttgcactcg tggcagacgt tcggaatcag
     3181 ttcgcccgta ccgccacagg tggcgcaggt ggccacgttg acaaactggc ccaggatcga
     3241 ctgctgcacg cgccgcacct cgcccgtgcc gccacaggtg gtgcaggcga tgggcttcgt
     3301 gcctggctca gccccattgc cgccgcaggt ggtgcacgtt tccgggcggc ggtactcgac
     3361 ctcgcgctcg acgccgaaga tagcctcctc gaacgtgatc gtcaggtcat agcgcaggtc
     3421 agcgcccttg cgcggcccgc gccggcggcg tcccctgccc gcgccggccc cgctgaaggt
     3481 gccgaagaac tcctcgaaga tgtcggcgaa cccgccgaag ccctgctcga agcccccata
     3541 gccgccattg ccaacgccgg cgtggccgaa gcggtcatag gctgagcgct tgtcgtcgtc
     3601 ggacagcacc tcataggctt cgctgatctc cttgaagcgc tcagccgctt ccggctcagg
     3661 gttcacgtcc gggtggtact gacgcgccag ccggcggtat tgcttcttga gttcgtcttt
     3721 ggttactgtc cgggagactt ccaggacttc gtaatagtcg cgcttgacag ccataggtag
     3781 ggcatcgggg ccaggttcca ggggccagaa aggaacgatg tctttctggc ccctggaacc
     3841 gaggctctaa ttggcgtcgc tgaactcgcc ttcaacaacg tcttcgtcgg agcggtcatt
     3901 ggccgggcca gagccggact ccgcgtctgg ctggccgccc tgattttgat acatggccgc
     3961 gccggccgac tgtaacgcct gctgtaggcg ctcggccgcc gcggtcatgc cggccgggtc
     4021 gccgctgccc atggccgact tga
//
LOCUS       DAOSMQ010000291         4001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22653_length_4001_cov_5.608464, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000291 DAOSMQ010000000
VERSION     DAOSMQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22653_length_4001_cov_5.608464"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..963)
                     /gene="prmC"
                     /locus_tag="PK829_09990"
     CDS             complement(127..963)
                     /gene="prmC"
                     /locus_tag="PK829_09990"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU41587.1"
                     /translation="MLTIRQARRDGRAQLHESPSPALDARLLLAHALGRDHAYLAAHD
                     DEALTAAQAAAYAQLLARAAAGEPIPYLLGRAPFFGLDFAVSPAVLIPRPETEQLVEA
                     AIAWGRGRGPLRAVDVGTGSGCIAVALARGLPAAAVAAVDTSAAALAVAAVNVARHAP
                     GGVALIRGDLLAAVAPRLDLIAANLPYVARSEWTALPDGVKSYEPALALDGGIDGLDI
                     IRGLLSQAATRLRPGGLLLLEIGWRQGPAAAALARAHFPTARIEVRPDFAGHDRFVAV
                     QT"
     gene            complement(1027..1584)
                     /gene="cysC"
                     /locus_tag="PK829_09995"
     CDS             complement(1027..1584)
                     /gene="cysC"
                     /locus_tag="PK829_09995"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOU41588.1"
                     /translation="MTQEYPGYVLWMTGLSGAGKTTIALLLETELQARGCKFERLDGD
                     VVRESLTRDLGFSKEDRDKNIERVSFVAKLLSRNGVGCVCSFISPYQAVRDMVRAGTT
                     NFIEVFIDAPLNVVMDRDVKGMYKKAIAGEIKNFTGISDPFEAPANPEIHIHTDQETP
                     AESAQRILTYLEERGFIPVREAAFA"
     gene            complement(1678..3405)
                     /locus_tag="PK829_10000"
     CDS             complement(1678..3405)
                     /locus_tag="PK829_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HOU41589.1"
                     /translation="MITPYGGKLVDLMVPQAEQAELRAYANTLPSIRISERAVCDLEL
                     LATGGFSPLDRFMSQADHQSVLDTMRLTNGYLFPMPIPLPVDAEDAARIKIGADIALR
                     SPANELLAILNVEEVYAWDLDEVAQKAFGTTDLRHPIVAEMHNWGKYYVSGPMRVLQL
                     PTHYDFRALRFTPVETRAKLERFGHHNVIAFQTRNPLHRVHEELTKRATMAKDGVLLL
                     HPVVGMTKPGDVDHFTRVRTYKALAQHYYDPDRILLSLLPLAMRMGGPREALWHAIIR
                     RNHGANFLIVGRDHAGPGNDSNGKPFYGPYDAQQMVEKYSDELGVGAVPFQMLVYLPE
                     EDRYEEVHKVTKETKTASISGTQVREQYLQVGRQLPEWFTRPEVAEILAETYPPRHRQ
                     GVCVWFTGLHNAGKSTTAGVLTTLLQEYGRNVTLLDGDVVRTHFSEGLGFSKEDRNDH
                     VRRIGFVASEVVRHGGIVICAAASPYRSTRNEVRNTIGGENYVEVFVDTPLEVCEQRD
                     TKGLYARARRGEIVGFTGIDDPYEAPQHPELTIETLSHSPEENARLVLNMLIEQGFVR
                     TEAPNLHDN"
     gene            complement(3786..>4001)
                     /locus_tag="PK829_10005"
     CDS             complement(3786..>4001)
                     /locus_tag="PK829_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41590.1"
                     /translation="VLQPDAGETHHWLEPLAVPSNGIEVERRWQLARDMAGQPVLWIQ
                     RQRRPLLSPPGRRLRFDVMVEAVEGVA"
BASE COUNT          666 a         1261 c         1354 g          720 t
ORIGIN      
        1 gcagcccacg gcgcaggtgt tcggcggcca gggcgacagc cgccgggtcg tccgcgtcaa
       61 gccgcagggt ggtcactgat tgacgatttg acataacagc acagtactgg ttgtcccttg
      121 tccggctcag gtctggacag ccacgaagcg gtcgtggccg gcgaaatcgg gccgcacctc
      181 aatgcgcgcc gtcgggaaat gcgcccgcgc cagcgccgct gccgccggtc cctgccgcca
      241 accgatttcc agcaacaaca gaccgccggg ccgcaggcgt gtggcggcct gggacaagag
      301 gccacggatg atgtccaggc catctatccc cccatccaga gccagagccg gctcgtagga
      361 ttttactcca tcgggcagcg ccgtccactc tgagcgggcc acgtagggca ggttggcggc
      421 gatcaggtcg aggcgagggg caaccgcggc cagcaagtcg ccgcggatga gagcgacgcc
      481 gccgggcgcg tggcgagcga cgttgacggc ggccacggcc agcgccgccg cggacgtgtc
      541 caccgcggcc acggccgccg ccggcagccc gcgcgccagg gccacggcga tgcaaccgct
      601 gcccgtgccg acatcgacag cgcgcagcgg gccacggccg cgcccccagg caatggccgc
      661 ctcgaccagt tgctctgtct ccggccgggg aatgagcacc gccggcgaga cggcgaagtc
      721 cagcccaaag aagggcgcgc ggccgagcag gtaggggatc ggctcgccgg cggcggcgcg
      781 ggccagcagc tgggcgtagg cggctgcctg cgccgctgtc agcgcctcgt cgtcgtgggc
      841 tgccaggtag gcgtgatcgc gccccagcgc gtgggccagc agcagccggg cgtcgagcgc
      901 cggcgagggc gattcgtgga gttgggcgcg gccgtcgcgc cgcgcctgtc gaatggtcag
      961 cataaacgca aacggcgacc ggcgcgtgtg cgtcccggtc gccgtctgcc aattctaccc
     1021 gactgtctag gcgaatgccg cctcgcggac ggggatgaag ccgcgctctt ccaggtaggt
     1081 caggatgcgc tgggcgctct cggccggcgt ttcctggtcg gtgtggatgt ggatttccgg
     1141 gttggccggg gcctcgaacg ggtcggagat accggtgaag ttcttgatct cgccagcgat
     1201 ggccttctta tacatgccct tcacgtcgcg gtccatgacc acattcagcg gcgcgtcgat
     1261 gaagacctcg atgaagttgg tcgtgccggc ccgcaccatg tcgcggacgg cctggtaggg
     1321 cgagatgaac gagcagacgc agccgacgcc gttgcgcgac agcagcttgg ccacgaagga
     1381 gacgcgctcg atgttcttgt cgcgatcttc cttgctaaag cccaggtcgc gggtcaggct
     1441 ctcgcgcacg acgtcgccgt caaggcgctc gaacttgcag ccgcgggcct gcaattccgt
     1501 ttccagaagc agggcgatgg tcgtcttgcc ggcgcccgaa aggccggtca tccacagaac
     1561 gtagccggga tattcttggg tcatgatggg ttttgtttcc tcgttgtcgt gattgcgttt
     1621 taaagtgggt cggtcgccgg ccggcgcatt gttcgcggcc ggcaaccgga tagggcttta
     1681 gttatcgtgc aggttcggcg cttcggtgcg aacaaagccc tgctcgatga gcatgttcag
     1741 caccagccgg gcattttctt ccgggctgtg gctcagcgtc tcgatggtca gctccgggtg
     1801 ctgcggcgct tcatacgggt cgtcaatgcc cgtgaagcca acgatctcgc cgcggcgggc
     1861 gcgggcatag aggcccttcg tgtcgcgctg ctcgcagact tccagcggtg tatcgacgaa
     1921 gacctcgacg tagttctcgc cgccgatggt gttgcggact tcgttgcgcg tggagcgata
     1981 ggggctggcc gcggcgcaaa tgacgatgcc gccgtggcgc acgacctcgg aggccacgaa
     2041 gccgatacgg cggacgtggt cgttgcggtc ttccttgctg aagcccaggc cctcagagaa
     2101 gtgggtgcgc acgacgtcgc cgtccagcag ggtcacatta cggccgtact cttgcagcag
     2161 cgtcgtcagc acgccagcgg tcgtcgattt gccggcattg tgcaggccgg tgaaccagac
     2221 gcagacgccc tggcggtggc gcggcgggta agtctcggcc aggatttcgg ctacttccgg
     2281 ccgcgtgaac cactccggca actgccggcc gacttgcagg tattgctcgc gcacctgcgt
     2341 gccagagatc gacgctgtct tcgtctcctt ggtgaccttg tggacttcct cgtagcggtc
     2401 ctcttcgggc aggtagacca gcatctggaa gggcaccgcg ccaacaccca gttcgtcgga
     2461 gtacttctcg accatctgct gcgcgtcata ggggccgtag aacggcttgc cgtttgagtc
     2521 gttgcccggg ccggcgtggt cgcggccgac gatcaggaag ttcgcgccgt ggttgcggcg
     2581 gataatggcg tgccagagcg cctcgcgcgg gccgcccata cgcatggcca acggcagcag
     2641 cgacagcagg atgcggtcgg ggtcgtagta gtgctgggcc agggccttat aggtgcgcac
     2701 acgggtgaag tgatccacat cgcccggctt ggtcatgccg acgacggggt ggagcaacag
     2761 gacgccgtcc ttcgccatcg tcgcccgctt ggtcagttcc tcgtgcacgc ggtgcagcgg
     2821 attgcgggtc tggaaggcga tgacgttgtg gtggccgaag cgctccagct tggcgcgggt
     2881 ctcgaccggc gtaaagcgca gggcacggaa atcgtagtgg gtgggcagct gcaagacgcg
     2941 catcgggccg gagacgtagt acttgcccca gttgtgcatc tcagcgacga tgggatgacg
     3001 caggtcggtc gtgccaaatg ccttctgggc aacctcatcc aggtcccagg cgtagacttc
     3061 ctcaacattg aggatagcca gtagctcgtt ggcggggctg cgcagggcga tgtccgcgcc
     3121 gatcttgata cgggccgcgt cttccgcgtc caccggcagg ggaatgggca tggggaacaa
     3181 gtagccgttt gtcaggcgca tcgtgtccag cacactctgg tggtcggcct ggctcataaa
     3241 gcgatcgagc ggcgagaagc cccccgtagc cagcaactcc aggtcgcata cagcgcgctc
     3301 agagatgcga atcgacggca gcgtattggc gtaggcccgc agttcggcct gttccgcctg
     3361 tggaaccatg aggtctacca gcttcccgcc atagggagtg atcaatgtag tcttttcagc
     3421 cgttgctaac aacgtaataa ctccttgtgc gccccctcgc gtcgctcgac acgctggcgc
     3481 acgcacacta ttttacgatc cgataccggc aaaatgatgg ccccgtggcc tgcgctaacc
     3541 caacggttgg caatcggaac acagcgatag actaccggcg gatgtcggta gcgcgacggc
     3601 tgtttacttt caacacggta cagttgcctg agtcggggaa agccgcgcaa gcggtgacaa
     3661 cacgggttat ctcaattccg taaacgttcg gtctgcgttt gcctgaatag taatctagtg
     3721 gctttttctt acaaccaaca ttaacgtttg ctgcgctggt tgcgctccag gctattggcc
     3781 ggcgatcatg ccactccctc caccgcctcc accatcacgt caaaccgtag ccgccgcccc
     3841 ggcggcgaca acaggggccg gcgctggcgc tgaatccaca ggaccggttg gccagccatg
     3901 tcgcgcgcca gttgccaacg ccgttcgacc tcgataccgt tactggggac ggccagcggc
     3961 tccagccagt ggtgcgtctc cccggcgtcg ggctgcaaga c
//
LOCUS       DAOSMQ010000292         4001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22654_length_4001_cov_5.392549, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000292 DAOSMQ010000000
VERSION     DAOSMQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22654_length_4001_cov_5.392549"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..2507)
                     /gene="scpA"
                     /locus_tag="PK829_10010"
                     /pseudo
     CDS             complement(320..2507)
                     /gene="scpA"
                     /locus_tag="PK829_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726854.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(2514..2816)
                     /locus_tag="PK829_10015"
     CDS             complement(2514..2816)
                     /locus_tag="PK829_10015"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOU41591.1"
                     /translation="MNAYKIYVTPSAWVEMRALPGHMRQRAKQSVNSLSRDPRPANSK
                     ALNIPETSYEVRRARLDRWRIVYFIDEETRTLSILTVRKRPPYNYQDLAELLRSLK"
     gene            complement(2813..3037)
                     /locus_tag="PK829_10020"
     CDS             complement(2813..3037)
                     /locus_tag="PK829_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015179279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41592.1"
                     /translation="MTGLDALQSVQYVNVEGKRFAVIDMDDWEALVEWLEDVEDSQIV
                     REALIQLQDADNDPRRAGWLDWDDIKNELS"
     gene            complement(3073..3382)
                     /locus_tag="PK829_10025"
                     /pseudo
     CDS             complement(3073..3382)
                     /locus_tag="PK829_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733868.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            complement(3388..>4001)
                     /locus_tag="PK829_10030"
     CDS             complement(3388..>4001)
                     /locus_tag="PK829_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41593.1"
                     /translation="RSQLATALRGDSAGALPTPAATPLPLARAAAPFEALRQKALSLT
                     LSQREREPDGERAQNPLALRERDGERVLPRLFLANLGPPRQHKARADFTQGFFEVGGF
                     QVITNNGFKTPEEAAAAALASGAPAVAICSTDETYPDLVPPLVAAIKAAAPNTVVILA
                     GRPAEQVEALRAAGVDEFVYLGMDCLAMNDWLLGQIMEREAQD"
BASE COUNT          630 a         1228 c         1387 g          756 t
ORIGIN      
        1 cgccactacg gcgattcgga gaatcgcgcc actacggcga ttcggagaat cgcgccacta
       61 cggcgattcg gagaatcacg ccactacggc gattcggaga atcacgccac tacggcgatt
      121 cggagaatcg cgccactacg gcgattcgga gaatcgcgcc actacggcga ttcggagaat
      181 cgcgccacta cggcgattcg gagaatcgcg ccactacggc gattcggaga gtcgcgccac
      241 tatggcgatt cggagagtcg cgccactacg gcgattcgga gagtcgcgcc actatggcga
      301 ttcggagaat cgcgccacgc tagggttggg gttggcccag gcggctcgct agttcggtca
      361 ggattctctc cgccgccacc ggcaccaccg tccctggccc aaagatggcc gtcgcgccgt
      421 gctcatagag atactcgtag tcctgcgccg ggatgacgcc gccgatgacg atcaggatgt
      481 cgtcgcggcc gaggcgggcc agttcggcgg ccagttgcgg cagcagcgtc ttgtgccccg
      541 cggctagact gctgacgccg acgacatgga cgtcgttctc catcgcctgg cgggcgactt
      601 cctccggtgt ctggaacagc gggcctatgt ctacgtcgaa gcccaggtcg gcgaaggcgg
      661 tggcgatgac cttggccccg cggtcgtggc catcctggcc catcttggcc acgaggatgc
      721 gcggccgccg cccctcggtc gcggcgaagg cgtcggccat ctcgcggacg cggccgatgg
      781 cgtcctgttc gccaaactcg gcgcgataga cgccggcaat ggaacggatc acggctttgt
      841 gcctccccca gacgctttcc agcgcgccgg aaatctcgcc cagcgtggcc cgggcgcggg
      901 cggcggcgac ggccagctcc agcaggttgc cctcgccggt ggccgcggcc tgcgtcagcg
      961 cgtcgagggc atgctcgacg gccgtcgcgt cgcgcccggc gcgcacctcg gccagtcgcg
     1021 ccacttgctg ctcgcacggc ggtgttgtcc acttccagga tgtcgattgg ggcttcctgg
     1081 tcaaggcgat acttgttgac gccgacgatg gtctcctggc cggagtcgat acgcgcctgg
     1141 cgacgggcgg cggcttcctc gatgcgcatc ttgggcaggc cgctctccag cgctttggcc
     1201 atgccgccca gttggtcgat ctcctggata tggccccagg cgcggcgggc cagatcgtgg
     1261 gtcagcgtct cgaccagata ggagccgccc cacgggtcaa cgatctgggt gatgcccgtc
     1321 tcgtgttgca ggtagagctg cgtgttgcgg gcgatgcggg cgctgaagtc ggtcggcagg
     1381 gcgatggcct cgtcgagggc gttggtgtgc agcgattggg tgtggcccag cacggcggcc
     1441 atcgcttcga tgcaggtgcg ggcgacgttg ttgaacgggt cttgctcggt caggctccag
     1501 ccgctggtct ggcagtgggt gcgcagggcc atcgacttcg ggtcttgcgg gttgaactgc
     1561 ttgatgagct tagcccacag tagccgcgcg gcgcgcatct tggcgatctc catgaagtag
     1621 ttcatgccga tagcccagaa gaaggacagg cgcggggcga agtcgtcgat ggccagcccg
     1681 gcgcgcaggc cggtgcggat gtactccagc ccgtcggcca gggtgtagcc cagttcgatg
     1741 tcggccgtgg ctccggcctc ctgcatgtgg tagccggaga tgctgatgct gttgaacctg
     1801 ggcatctcgc cggcggtgta gctgaagatg tcgccgatga tgcgcatgga tggctcgggc
     1861 gggtagatgt aggtgttgcg caccatgtac tctttgagga tgtcgttctg gatcgtgccc
     1921 gtcagttggg cgtggccgac accctgctct tcggcggcga cgatgtagaa ggccatgacc
     1981 ggcagcaccg cgccattcat ggtcatggag accgacatct tgtccagcgg aatgccgtcg
     2041 aagaggatct gcatgtcgcg cagggaactg atggccacgc cggccttgcc cacgtcgccg
     2101 acgacgcgcg ggtgatcgct gtcgtagccg cggtgggtgg ccaggtcgaa ggcgacgctc
     2161 aggccgcgct ggccgccggc caggttgcgg cggtagaagg cgttgctctc ctcggccgtg
     2221 ctgaagccgg catactggcg caccgtccac ggccggccga cgtacatggt cgggtatggc
     2281 ccgcgcagga agggcggccg gccggcggcg ttgtcgaagt tgggcagggc ggccgtgtcg
     2341 ctggcggtat agagcgggcg cacgtcgatc tgctccatcg tgcgccagag gaagtcggcc
     2401 gccggctggc cggtcgcggc ctccagggcg gcttgccagt cggccagcga ctgcggcgcg
     2461 ggcgcggcgg cggggtaggg gagttgggtg aagtcggggt tggtcatggg gttctatttg
     2521 agcgagcgta gtagctcggc caagtcttgg tagttatagg gcggacgttt ccgcaccgtc
     2581 aggatgctca aggtgcgcgt ctcttcatca ataaaataga cgattcgcca gcgatccagg
     2641 cgcgcccgac gcacttcgta ggaagtctca ggaatgttca gggccttgct gttggcaggt
     2701 cgcgggtctc tggaaaggga gttcacagac tgcttcgcgc gctgccgcat atggcctggc
     2761 aatgcgcgca tttccaccca cgccgatggc gtgacgtaga tcttataggc gttcatgaca
     2821 actcgttctt gatgtcatcc caatcaagcc acccggcgcg ccgaggatca ttgtcggcgt
     2881 cctgcagttg aatgagcgct tcgcggacaa tctggctgtc ttcaacgtcc tcaagccact
     2941 caaccagcgc ttcccagtcg tccatgtcga tgacagcaaa gcgcttgcct tcaacgttca
     3001 cgtattgcac agattgtagg gcgtcaagtc cggtcatgtt cttgctcctg gtgccacggt
     3061 tagctgcaca ttctaggttg ctgtcgaata gaccttaact tcttgctcca gacgtccctt
     3121 cagccgatcc ttttccactt ccagatcgta acgaccttgc cgggttcatc cagaagcgct
     3181 ctgacaggcc aaagtagcgc gagaggcgca gggccgtgtc ggcggtgata cggcgtttgc
     3241 ccagcacaat ttcattgatg cgtcgaacgg gaacaccaat gtcttttgcc agccggtact
     3301 ggctgactcc cagtggttga aggaactctt ccagtagtat ctcaccgggg taaatgggcg
     3361 tgagcaagtc gttgtcttcc atgtggctta gtcttgcgct tcgcgttcca tgatctgccc
     3421 cagcaaccag tcattcatcg ccaaacaatc catccccaaa tagacgaact catccacccc
     3481 cgccgcccgc agcgcctcca cctgctcggc cgggcgaccg gccaggatga caacggtgtt
     3541 gggcgccgcg gccttgatgg cggcgacgag gggcgggacg aggtcggggt aggtctcgtc
     3601 ggtggagcag atggccacgg ccggcgcgcc ggaggcgagg gcggcggcgg cggcctcttc
     3661 gggcgtcttg aagccgttgt tggtgatgac ctggaagccg ccgacctcga agaagccctg
     3721 ggtgaagtcg gcgcgggcct tgtgctggcg cggcgggccg aggttggcca ggaagaggcg
     3781 gggcagaacc ctctccccat ccctctcccg gagggcgagg gggttttgcg ctctctcccc
     3841 gtctggttcc ctctccctct gggagagggt tagggagagg gccttttgcc gcagcgcctc
     3901 aaacggcgcg gcagcgcggg cgaggggcag cggcgtggcc gccggcgtgg gcagcgcgcc
     3961 ggccgagtcg ccgcgcaggg ccgtagccag ttgcgaccgc g
//
LOCUS       DAOSMQ010000293         3982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22801_length_3982_cov_4.706137, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000293 DAOSMQ010000000
VERSION     DAOSMQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22801_length_3982_cov_4.706137"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..668)
                     /locus_tag="PK829_10035"
     CDS             complement(147..668)
                     /locus_tag="PK829_10035"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOU41594.1"
                     /translation="MTAPRIAYVVALDANRLIGRDNALPWRLPDDMRWFREQTLGKPC
                     IMGRKTYDSLPERFRPLPGRRNIVLTRDRDYVAPGALVVHSLDDALAAAGAAEEIIIV
                     GGAELFRALLPRVDRLYLTQIHAAVEGADTATDVFFPAFDADEWRVVYRQAHPADERH
                     PFAFTWLILDRRG"
     gene            complement(684..1478)
                     /locus_tag="PK829_10040"
     CDS             complement(684..1478)
                     /locus_tag="PK829_10040"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOU41595.1"
                     /translation="MRQYLDLMQHILDHGVRKEDRTGVGTISVFGHQMRFDLAAGFPL
                     LTTKKMHLRSIIYELLWFLRGETNVGYLRAHNVTIWDEWADADGELGPVYGRQWRSWP
                     TADGGAVDQIAALVAQIDRNPNSRRLIVSAWNVGEIEHMALPPCHCLFQFYVADGRLS
                     CQLYQRSADVFLGVPFNIASYALLTQMLAQVTGYAPGEFIHTLGDAHLYLNHLEQARL
                     QLGRVPLPLPAMRLNPAVTSLFDFCYEDFALVDYHSHPRIAAPIAV"
     gene            1659..1741
                     /locus_tag="PK829_10045"
     tRNA            1659..1741
                     /locus_tag="PK829_10045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1692..1694,aa:Leu,seq:taa)
     gene            1745..2668
                     /locus_tag="PK829_10050"
     CDS             1745..2668
                     /locus_tag="PK829_10050"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU41596.1"
                     /translation="MNDLKARYRGCLLGLAVGDAVGTTVEFSPPGSFAPVADMVGGGP
                     FRLQAGEWTDDTSMALCLAESLLERDGFDAADQMDRYLRWYRHGHLSSNGRCFDIGNT
                     VSSALRRYEQTGDPWAGSTDAYSAGNGSIMRLATVPLFFAADATRAVAMSGESSRTTH
                     GAPACVDACRYLGGLIVGALRGEPKDTLLSPRYCPAGAEPWAGLDPDIAAIADGSFAR
                     RSPPDIVGSGYVVRSLEAALWAFHRADSFEEGCLLAVNLGDDADTTAAVYGQLAGAFY
                     GEAGIPARWCARLAQGALITGFADRLYEAAQ"
     gene            2773..>3982
                     /gene="tilS"
                     /locus_tag="PK829_10055"
     CDS             2773..>3982
                     /gene="tilS"
                     /locus_tag="PK829_10055"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU41597.1"
                     /translation="MSAFVGQVAAALEAALGAAGLAGSSGSLVVGVSGGPDSLALLDC
                     LAQLLPAERLVVAHLDHGLRPSAAAEAALVAEAAARRGLRHVGGRADVPALAREAGLS
                     LETAARAARYDFLAAVAHAEEAAAVVVGHNADDQAETILMHLLRGAGAAGLRGMAAAA
                     PLPGHADLWLLRPLLGIGRADIGAYCRDAGLEPAADASNLDPSFTRNRLRHELLPVLA
                     AHAPHTAQRLREAGILLAAEDDLLSALEDDAWQTIARQTPASIALSRAAWREQPLALR
                     RRLLRRAVAAVAAAPVELSFRSIEAARRLAEGRASGGRVALAGGVVMVVGYETLDFGR
                     GPATTSDDWPQLTAGEAVSLPIPGAVALAGGWRLLAEPLPALDYDAIRANADPWTVTI
                     ALERPQPLLVR"
BASE COUNT          590 a         1375 c         1409 g          608 t
ORIGIN      
        1 gcccgcgccc tggcgttcga cctggccgcg caggacgacg cgaaccatct gacgcggctg
       61 gcagccgggc ggatcgcggt ggcggttgag ttggcggact aggggatagg ggctagggaa
      121 gagcgctctt ccccagcccc tatcccctat cccctacggt cgaggatgag ccacgtgaac
      181 gcgaacgggt ggcgctcgtc ggccgggtgg gcctggcgat agacgacgcg ccactcgtcc
      241 gcgtcgaagg ccgggaagaa gacgtcggtg gcggtgtccg cgccctcgac cgcggcgtgg
      301 atctgcgtca ggtagagccg gtcgacgcgc ggcaacaggg cgcggaacag ctccgccccg
      361 ccgacgatga tgatctcctc ggccgcgccg gccgcggcca gagcgtcatc gagcgagtgg
      421 acgacgagcg cgccgggggc gacatagtcg cggtcgcgcg tcaggacgat gttgcgccgc
      481 cccggcagcg gccggaagcg ctccggcagc gagtcatacg tcttgcggcc catgatgcac
      541 ggcttgccca gcgtctgctc gcggaaccag cgcatgtcgt cgggcagccg ccacggcagg
      601 gcattgtcgc ggccaatgag ccggttggcg tccagggcga cgacgtaggc gatgcgcggc
      661 gcggtcatcg tctctccccg gcgctagacg gcgatgggcg cggcgatgcg cgggtggctg
      721 tggtaatcga caagcgcgaa gtcctcatag caaaagtcga agagcgacgt gaccgccggg
      781 ttcaggcgca tggccggcag cggcaggggg acgcggccga gctgcaaccg ggcctgctcc
      841 aggtggttaa ggtatagatg ggcgtcaccc agagtatgga tgaactcgcc cggcgcgtag
      901 ccggtcacct gggccagcat ttgcgtcagg agggcatagg aggcaatgtt gaagggcacg
      961 cccaggaaga cgtcggcgct gcgctggtag agctggcacg acaggcggcc gtcggccacg
     1021 tagaactgga acaggcagtg gcacggcggc agggccatgt gctcgatctc gcccacgttc
     1081 caggcgctga cgatgagccg gcgcgagttg gggttgcggt cgatttgcgc caccagcgcg
     1141 gcgatctggt caaccgcgcc gccgtcggcc gtgggccagg agcgccattg ccgcccgtag
     1201 accggcccca gttcgccgtc ggcgtcggcc cactcgtccc agatggtgac gttgtgggcg
     1261 cgcagatagc cgacattggt ctcgccgcgc aggaaccaca gcagctcgta gatgatcgac
     1321 cgcaggtgca tcttcttggt cgtcagcagg ggaaacccgg cggccagatc gaagcgcatc
     1381 tggtggccga agacgctgat cgtgcccacg ccggtgcgat cctccttgcg gacgccgtgg
     1441 tcgaggatgt gttgcatcag gtcaaggtac tgtcgcatgt cgcctcccgt tacaggtcgg
     1501 cgggagtata gcacaagggc gggacagaac atggataatg cagagaagtg gccagtgggc
     1561 agtggccagt cgcactgttc actgttcact gtctactggt cactctccac tcttcggttg
     1621 taggaagggg ccggtgaaag tagaataagc tctctgtcgg ggcggtggcg aaacggcaga
     1681 cgcagcggac ttaaaatccg tcgggctaac acccgtgtgg gttcgactcc cacccgccct
     1741 acctatgaac gatctcaagg cgcgctatcg aggctgcctg ctgggcctgg ccgtgggcga
     1801 tgccgtcggc acgaccgttg aattcagccc accaggctcc ttcgcgcccg tcgccgacat
     1861 ggttggcggc ggccctttcc ggctgcaagc gggcgaatgg accgacgaca cgtcaatggc
     1921 cctctgcctg gccgagagcc tgctcgaacg cgacggcttc gacgccgctg accagatgga
     1981 tcgctacctg cgctggtatc gccacggcca cttgtcctcc aacggccgct gcttcgacat
     2041 cggcaacacc gtgagcagcg cgttgcgccg ctacgagcag acgggtgacc cctgggccgg
     2101 atcgaccgac gcctatagcg ccggcaatgg cagcatcatg cgcctggcca ccgtgccgct
     2161 gttctttgcc gccgatgcca cccgcgccgt ggccatgagc ggcgaaagct cgcgcaccac
     2221 ccacggcgcg ccggcctgtg tcgatgcctg ccgctacctc ggcgggctga tcgtcggcgc
     2281 gctgcgcggc gagccgaagg acacgctgct atcgccgcgc tactgccccg cgggcgctga
     2341 accgtgggcc ggcctcgacc ccgacatcgc cgccatcgcc gatggttcct ttgcccgccg
     2401 ctcgccgcca gacatcgtgg gcagtggcta cgtcgtccgg tcgctggagg cggcgctgtg
     2461 ggccttccac cgcgccgatt ccttcgagga gggctgcctg ctggccgtca acctgggcga
     2521 cgacgccgac acgacggcag ccgtctatgg ccagctggcc ggggctttct atggcgaggc
     2581 gggcatcccc gcgcgctggt gcgcgcggct ggcccagggc gcgctcatca ccggcttcgc
     2641 cgaccggctc tacgaggccg cccaatgacc gccgacgact tccgccgcct ctacgactac
     2701 cacttctatc tcaatcagcg gacaaagttc ccgcaagact acgccctctg ccgcctgggc
     2761 aggctctaga ccatgagcgc cttcgtcggc caggtcgccg cggcactgga ggccgcgttg
     2821 ggagcggccg ggctggccgg tagcagcggg tcgctcgtcg tcggcgtctc cggcggtccg
     2881 gactcgctgg ctttgctcga ttgcctggcc cagctgttgc cggccgagcg gcttgtcgtc
     2941 gcccacctcg accacggcct gcggcccagc gccgccgccg aggccgcgct ggtggcggag
     3001 gcggcggcca gacgtgggtt gcgccacgtg ggcggccggg ccgatgtacc cgcgctggcc
     3061 cgcgaggccg ggctatcgct ggagacggcg gcgcgcgccg cccgctacga cttcctggcc
     3121 gcggtggccc acgccgaaga agccgcggcc gtcgtcgtcg gccacaacgc tgacgatcag
     3181 gccgagacca tcctcatgca cctgctgcgg ggcgcgggcg cggccgggct gcggggcatg
     3241 gccgcggccg cgcccctgcc cggccacgcc gatctatggc tgctgcggcc gttgctgggc
     3301 atcggccggg cggacatcgg ggcctactgt cgcgacgccg gcctggagcc ggccgccgac
     3361 gccagcaacc tcgacccgtc cttcacgcgc aaccggctgc gccatgagtt actgcctgtg
     3421 ttggccgctc atgcccctca taccgcccag agactacgcg aagcaggtat cctcctggcc
     3481 gccgaggatg acctcctatc agcgctggaa gacgacgcct ggcagaccat cgcccggcaa
     3541 accccggcca gcatcgccct cagccgcgcg gcctggcgcg agcaaccgct ggccctgcgc
     3601 cgccgcctgc tgcgccgggc agtggccgct gttgccgccg cgccggtgga gttgagcttt
     3661 cgcagcatcg aagcggcgcg gcgactggcc gaggggcggg ccagcggcgg gcgcgtggcc
     3721 ctggccggcg gcgtggtcat ggtcgtgggc tacgagacgc tcgactttgg ccgcggcccg
     3781 gcgacaacaa gcgatgactg gccgcaacta acggctggcg aggctgtgtc gctgcccatt
     3841 cccggcgcgg tggccctggc cggcggctgg cggttgctgg ccgagccgtt gccggcgctc
     3901 gactacgatg ccatccgtgc caacgccgac ccgtggacag tcacaatcgc cctggaacgg
     3961 ccccagccgc tacttgtccg gc
//
LOCUS       DAOSMQ010000294         3980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22816_length_3980_cov_4.453758, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000294 DAOSMQ010000000
VERSION     DAOSMQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22816_length_3980_cov_4.453758"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="cas6"
                     /locus_tag="PK829_10060"
     CDS             <1..667
                     /gene="cas6"
                     /locus_tag="PK829_10060"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOU41598.1"
                     /translation="PYVVVAAAEVGRVGVGRDRRDGQGRFRLEAIDSHNPFTGDTLQL
                     LAPGDPVVHANPAVITFDHIRPAADRLCAGLAQDDNTLQLRFLSPTRLEEKDRLFRAP
                     DFAVLFRRLLYRIDDLALNFCGGVRRDRAELDYLHAAADRVRLVACDVRWVEQWSWSG
                     RKESRTPVGGFVGTAAYRSDDWADLLPWLLLGQATHVGKLPVKGNGVYEVSSQANSYW
                     PWR"
     gene            complement(681..1223)
                     /locus_tag="PK829_10065"
     CDS             complement(681..1223)
                     /locus_tag="PK829_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41599.1"
                     /translation="MTPDEELTTMLTDFFGLPGGQWPARALLQVDRAAGSVVVVQPQP
                     CDCLPAAGATLFPADAADCANAARADAEVFRYDYLAQDPARLDGLSAADLNNIACTLL
                     WQSDASPESARAAHDLLTRARARCADGDPLRDLIDRNLDKFRPVAPGVPRPLAMLQSV
                     VYDSGRAMGSLEEWTIEKEG"
     gene            complement(1244..2938)
                     /locus_tag="PK829_10070"
     CDS             complement(1244..2938)
                     /locus_tag="PK829_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41600.1"
                     /translation="MQLPILGTLSALFSDAGDEFKKLLEPWALIAAAALLALGLVFFY
                     LPLRAGSRALLAFENWPAAWQVVVATALLFALAYLINTLSGFFLALAGGRPLRDVPWL
                     GARLRRRQASRFDRLLATATTAAGEASARAFQRLAYEMPGRDELAPTALGNVRAAAAA
                     YSANQYGAHLDTVWPLVALTLAGKDEELLGQLRGEQTATTFLTTVAVLLALVSVPALL
                     LALAQGAAGRALGAAALLLPAYGLYRAAIVRALGWGRLTRAALDLYLDATGAKLGLRP
                     LEGEPAGARARWEAVSQWLAYGGLRPGDARVQIPLAQSDWYKTADAPAPARLTAAAGV
                     AAELRVAARPVVAYALLPNGLRLPEAAYDVTVLAAPALSAPPVSPAATAAPAAGGFVL
                     LTDARLPYLSPTMGALLYPDGTTATVRPLIAPGDGDPGESRDAYLWPLPGLAAQGSAV
                     LTYRAAVETWLRAGPGVALLSVAPNPSNRPDQLAVTLTYNGSPGGQAMLTCDPIAAGR
                     AGAAFQWAALSGGLPGPATSVAVAYDPAARRATWAIDNAPPGDIFVVLTLTGHGLA"
     gene            complement(3020..3853)
                     /locus_tag="PK829_10075"
     CDS             complement(3020..3853)
                     /locus_tag="PK829_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41601.1"
                     /translation="MIRTVATNVWAFDLEWVPDPPSGRRVYDLPADMPDDEVLAVMWA
                     NGGASDDEPQPFLKYTLCRVVSAAVVVRRQRPDGRVSVALAALPHVGDPPLPERDLIS
                     RFLTQLGQEDPKPQLVGFNSRTADVPILVQRGIVNGISAVEFCRRPGKPWEGVDYFAK
                     GHDWHVDIKEIVSGWGKGTPSLHEFAAACGIPGKIGTTGHDVHALWRAGDIRRIVEYN
                     QFDALTTYLVWLRMALFAGLLSPEIHACEEEYVRELLARRVAAGDGYLQAFADKWAAL
                     N"
BASE COUNT          588 a         1467 c         1407 g          518 t
ORIGIN      
        1 gccctacgtc gtcgtcgccg ccgccgaggt cggtcgggtc ggcgtcggcc gcgaccggcg
       61 cgacggccag ggccgcttcc ggctggaggc catcgactcc cacaacccct tcaccggcga
      121 caccctccag ctcctcgccc ccggcgatcc cgtcgtccac gccaatccgg ccgtcatcac
      181 cttcgaccac atccgccccg cggccgaccg gctttgcgcc ggcctggccc aggacgacaa
      241 caccctgcaa ctgcgcttcc tcagccccac ccggctggag gaaaaagacc ggctcttccg
      301 cgcccccgat ttcgccgtcc tcttccgtcg cctcctctat cgcatcgacg acctggccct
      361 caacttctgc ggcggcgtgc ggcgcgaccg ggccgagctt gactacctcc acgccgccgc
      421 cgaccgcgtt cgcctggtcg cgtgcgacgt ccgctgggta gagcagtgga gctggtcggg
      481 ccgcaaggag agccgcacac cggtgggcgg cttcgtgggc acggccgcct atcgcagcga
      541 cgactgggcc gacctgctgc cctggctgct gttgggccag gccacccacg tgggcaagct
      601 cccggtcaag ggcaacgggg tctatgaggt ctcaagtcag gccaatagct actggccctg
      661 gcgttaacct cttacgctcc ctacccctcc ttctcaatcg tccactcctc caggctgccc
      721 atcgcccgcc cggagtcata gaccacgctc tgcaacatgg ccagcggccg gggcacgccc
      781 ggcgcgaccg gccggaactt gtccagattg cggtcgatga ggtcgcgcag cgggtcgccg
      841 tcggcgcacc gggcgcgggc gcgcgtcagc aagtcgtggg ccgcccgggc gctctccggc
      901 gaggcgtcgg actgccacag cagggtgcag gcgatgttgt tcaggtcggc cgccgacagg
      961 ccgtcgagcc gggccgggtc ctgggccaga tagtcgtagc gaaagacctc ggcatcggcg
     1021 cgggcggcgt tggcgcagtc ggccgcgtcg gccggaaaga gcgtcgcccc ggcggccggg
     1081 aggcaatcgc acggctgcgg ctggacgacg acgacgctgc ccgcggcacg atcgacctgc
     1141 aacagcgccc gcgccggcca ttggccaccc ggcagcccga agaaatccgt cagcatcgtg
     1201 gtcagttcct catcgggggt cattcgtcgc tccttcccgc ggctcaggcg aggccgtggc
     1261 cggtcagggt cagcaccaca aagatgtcgc cgggcggcgc gttgtcgatg gcccaggtgg
     1321 cccggcgggc ggccgggtca taggccacgg ccaccgacgt cgccgggccg ggcaggccgc
     1381 cgctcagcgc cgcccattga aacgccgcgc cggcgcggcc ggcggcgatg gggtcgcagg
     1441 tcaacatcgc ctggccgccc ggcgagccgt tataggtcag tgtcaccgcc agctggtcgg
     1501 gccggttaga cgggttgggg gccacggaca gcagagccac gcccggcccg gcccgcagcc
     1561 acgtctccac cgccgcgcgg taggtcaaca ccgcgctgcc ctgcgccgcc agtcccggca
     1621 gcggccacag gtaggcgtcg cggctttcgc cgggatcacc gtcaccggga gcgatgagcg
     1681 gcctgacagt cgccgtcgtg ccgtcggggt acaggagcgc gcccatcgtg ggcgacagat
     1741 agggcaggcg cgcgtcggtg agcaggacga agccgccggc ggccggggcc gcggtcgccg
     1801 cgggcgagac gggcggggcg ctcagagccg gcgcggccag cacggtcacg tcgtaggcgg
     1861 cctccggcag gcgcaacccg ttgggcaaca gggcgtaggc gaccaccggc cgggccgcca
     1921 cgcgcaactc cgcggccacg ccggccgcgg cggtcagcct ggccggggcg ggcgcgtcgg
     1981 cggttttgta ccagtcgctc tgggccaacg gaatctgcac gcgggcgtcg cccggccgca
     2041 gcccgccata ggccaaccac tgcgacaccg cctcccagcg cgcccgcgcg ccggccggct
     2101 cgccctccag cgggcgcagg cccagcttcg cgccggtggc gtcgaggtag aggtcgagcg
     2161 ccgcccgcgt cagccgcccc cagcccaacg cgcgcacgat ggcggcgcgg tagaggccat
     2221 aggcgggcag cagcagagcc gcggccccca gcgcccgccc ggccgccccc tgcgccaggg
     2281 ccagcagcag cgccgggaca gacaccaggg ccagcagcac ggccactgtc gtcagaaagg
     2341 tcgtcgccgt ctgctcgccg cgcagctggc cgagcaactc ctcgtccttg ccggccagcg
     2401 tcagagcgac gaggggccag acggtgtcga ggtgcgcgcc atactggttg gcgctgtagg
     2461 ccgccgccgc ggcgcggacg ttgccgaggg ccgtcggggc cagttcgtcg cggccgggca
     2521 tctcgtaggc cagccgctgg aaggcgcggg cgctggcctc gccggcggcg gtggtggccg
     2581 tggccagcag ccggtcgaag cggctggcct gccgccggcg cagcctcgcg cccagccagg
     2641 gcacgtcacg cagcggccgc ccgccggcca gggccaggaa gaaaccgctg agggtgttga
     2701 tgaggtaggc cagcgcgaag agcagcgccg tggccacgac cacctgccag gccgccggcc
     2761 agttctcgaa ggccagcagg gcgcggctgc cggcccgcag gggcagatag aaaaagacca
     2821 gccccagggc cagcagcgcc gccgcggcga tgagcgccca cggctccagc agcttcttga
     2881 attcgtcgcc cgcgtcgctg aacagcgccg agagtgtgcc gaggatgggt agttgcaccg
     2941 cgccacctcc ttcagcaggg attgccatgc ccacgaaagg ccaccgcggg ccggaaagac
     3001 cactgcggtc gggccgaact cagttcagcg ccgcccactt gtccgcgaag gcctgcaaat
     3061 agccgtcgcc cgcggccacg cgccgggcga gcaactcgcg gacgtactcc tcctcgcagg
     3121 cgtggatttc cggcgacagc agcccggcaa acagggccat acgcagccag acgagatagg
     3181 tcgtcagggc gtcgaactgg ttgtactcga caatccggcg gatatcgccc gcgcgccaga
     3241 gggcgtgcac gtcatggccg gtcgtgccga tcttgccggg aatgccacag gcggcggcga
     3301 actcgtgcag cgacggcgtg cccttgcccc agccggagac gatctccttg atgtccacgt
     3361 gccagtcgtg gcccttggca aagtagtcca cgccttccca cggcttgccc ggccgccggc
     3421 aaaactccac cgcgctgatg ccgttgacga tgccgcgctg caccagaatg ggcacgtcgg
     3481 ccgtgcgcga attgaagccc acgagctgcg gcttggggtc ttcctggccc agctgggtga
     3541 ggaagcggct gatcaggtcg cgctccggca ggggcgggtc gccaacgtgg ggcagcgcgg
     3601 ccagggcgac gctgacgcgg ccatcggggc gctgccgccg cacgaccacg gcggccgaga
     3661 caacgcggca aagggtgtac ttgaggaagg gctgcggctc gtcgtcgctg gcgccgccgt
     3721 tggcccacat gaccgccaat acttcatcgt cgggcatgtc ggcgggcagg tcgtagacgc
     3781 ggcggccgct gggtgggtcg ggaacccact ctaaatcgaa ggcccacacg ttggtggcga
     3841 cagtgcgaat cattactggc ctcctgtggc gggcaattgc ccaccttgcc gacagtatac
     3901 gacccggctg ccgtcgcgtc aattctcccc gtggcgcgat tctcccgaat cgccacccca
     3961 tccccctgtg gcgcgatcct
//
LOCUS       DAOSMQ010000295         3978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22828_length_3978_cov_3.987000, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000295 DAOSMQ010000000
VERSION     DAOSMQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22828_length_3978_cov_3.987000"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK829_10080"
     CDS             <1..186
                     /locus_tag="PK829_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HOU41602.1"
                     /translation="LLVSPYPEQRSPIVMTGWGVQLELDSIDDDRVGQFVERYRVGPN
                     TPERGAACTGGTGEPLS"
     gene            complement(265..1197)
                     /locus_tag="PK829_10085"
     CDS             complement(265..1197)
                     /locus_tag="PK829_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41603.1"
                     /translation="MSTTRFTANDFRAPVVRRCLAALALLLLIALVACGDDTPAGEPT
                     LAPASQATTVPQAAAPTEAPPAEATATTALPTEAPPTEAPAAATATAAPTAAATVPVA
                     AGECDNPFFPAVEGRVMTYRNTVPTFGESEFSHTFSDVTASSFNLNIGVGDGESVVQK
                     WTCSADGLLSPEMAQMPGMAEGMTFNYLEASGATLPAADQMTAGHEWTTHYVVEVTMP
                     DLGQGAMSMSETVDLVNTVAGEESVTVPAGTFDAMRVDTTGTIAVSIAGAPASNIEMS
                     MASWYAEGVGLVRQEIGGMLGGEDTIVTELVSVE"
     gene            complement(1327..2058)
                     /locus_tag="PK829_10090"
     CDS             complement(1327..2058)
                     /locus_tag="PK829_10090"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOU41604.1"
                     /translation="MSLKKLFVIGLAMLSVAILVGCSALSDLNFVTGSGNTVTREFDL
                     SDFDEVSIENAFQGTITRGDNYRVVVTFDDNLEDLLNVDVSDSRLTIGFTKPTAVTNA
                     NLSYEIIMPALKVVEASGASQAQIAGFSSGDALRAEASGASRIEGDISSGDADLNASG
                     ASTILLIGTGGDLRGEASGASTLDLTDFVTADADVVASGASRIIVNTTGRLDAEASGA
                     SNIQYQGQPTLGNIDASGGSNINPQ"
     gene            2223..3572
                     /locus_tag="PK829_10095"
     CDS             2223..3572
                     /locus_tag="PK829_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOU41605.1"
                     /translation="MTADNKTTLPATRLGWGESRALSLDRIGWMIRSAAAQGVSHYKV
                     LWFDVFFVADPDVVRELIVKRPHLLHRDPFTTNVLRRIMGNGVFIAEDEAWQRQRKLV
                     APAFHAMRIRAYAETMAAFTREMAGRWRDGEVVPLGRELTALTLRIIAKTMFNVDLLP
                     EAERIGRLMQALLETGEKQLDAAYPLPNWVPTPTNRRQNAALRELHALVRDIIRARQA
                     SGRDEGDLLSMLVQARDDEGRPMSEAQIIDECVTLLTAGHETTAVALMWTWLLLGQRP
                     DVADGLVAEVDSVLGGAPVTFELLAALPITESIIKETLRLYPPAFSFGRTPQEAIALE
                     INGQSYFFRKGSSLIMSTIAMHRLPEYFPEPDAFRPERWASGEAAPPKYAYLPFGGGP
                     RVCLGNLFAMMEAQVILATMAQHVRLSPVEPGLPPWSPLITLRPKGEVRMRVARRTP"
     gene            3614..>3978
                     /locus_tag="PK829_10100"
     CDS             3614..>3978
                     /locus_tag="PK829_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41606.1"
                     /translation="MSDRLALRLLRWSPRVLAILYATFLGLFALDAFGSGSRLEQVVG
                     FLIHLAPVYAVVLALIFAWRWPRLGGVLFVALAVGFVFAFGWRGLGTVVVMAGPPVAA
                     GVLFLASGWGGRARLRARF"
BASE COUNT          598 a         1281 c         1366 g          733 t
ORIGIN      
        1 ctcctggtca gtccctatcc ggagcagcgc agccccattg tcatgaccgg ctggggcgtg
       61 cagctggaac tggacagcat cgatgacgac cgcgtcggcc agttcgtcga gcgctaccgc
      121 gttggcccga atacgccgga gcgcggcgcg gcctgcaccg gcggcacggg cgagccgctc
      181 agctaaccgg ccgccggaca tccgcccgtc gtgaccggcc aggcaggcta ctcgcccgcc
      241 tggccggtct ttttcatgtc gcggctactc gaccgacacc aactcagtca cgatagtgtc
      301 ctcgccgccc agcatgccgc cgatctcctg ccgcaccaga ccaacgccct cggcatacca
      361 cgacgccatg ctcatctcga tgttgctggc cggcgcgccg gcaatgctga cggcgatcgt
      421 gcccgtcgtg tccacgcgca tggcgtcgaa cgtccccgcc ggcacggtga ccgactcctc
      481 gccggcgacg gtgttcacca gatcgactgt ctccgacata gacatggccc cctggcccag
      541 gtcgggcatt gtgacctcga cgacgtagtg ggttgtccac tcgtggccgg cggtcatctg
      601 gtcggcggcc ggcaacgtcg ccccgctggc ctcgaggtag ttgaaggtca tcccctcggc
      661 catgccgggc atctgcgcca tttccggcga cagcaggcca tcggccgagc atgtccactt
      721 ctggacgacg ctctcgccgt cgcccacgcc gatgttgagg ttgaagctgc tagcggtgac
      781 atcactaaag gtgtggctga attccgactc gccaaaggtg ggcacggtgt tgcggtaggt
      841 catgacccgc ccctcgacgg ccgggaagaa ggggttgtca cactcgccgg cggcgacggg
      901 cacagttgcg gccgccgttg gggcggcggt ggctgtcgcc gcggccgggg cttcagtcgg
      961 cggggcttcg gttggcaggg ccgtggtcgc cgtcgcttcc gccggtgggg cctcggttgg
     1021 cgcggctgct tgcggcacgg tcgttgcctg agacgccggg gccagggtcg gctcgcccgc
     1081 gggcgtgtcg tcgccgcagg ccaccagggc gatcagcagc agcaaggcca atgccgccag
     1141 gcagcgacga accacaggag cacgaaagtc gttggctgta aacctggttg tagacatggg
     1201 cgcattgtaa gggacggcca cgcccggcgt ctatgggcgg aatgtccgga ttggcgcaga
     1261 aaaaggggcg ggccgggtcg cgccggcccg ccccgcgtgg gcgatggtcg cgggcgcgcc
     1321 ggcggcttac tgggggttga tgttgctgcc gccgctggcg tcgatgttgc ccagcgttgg
     1381 ttggccctgg tattgaatgt tagaggcgcc ggacgcttca gcgtccagcc gcccggttgt
     1441 gttgacgatg atcctgctgg ccccgctggc caccacatcg gcgtcggcgg tgacgaagtc
     1501 ggtcaggtcg agcgtgctgg ctccgctggc ttcgccgcgc aggtcgccgc ctgtgccgat
     1561 gaggagaata gtgctggcgc cgctggcgtt taggtcggcg tcgccgctgc tgatgtcgcc
     1621 ctcgatgcgg ctggccccgc tggcctcggc ccgcagggcg tcgccggaac tgaagccggc
     1681 gatctgcgcc tgactggctc cgctggcctc cacgaccttt agcgcgggca tgatgatctc
     1741 gtagctcaag ttggcgttcg tgacggccgt gggcttcgtg aagccaatgg tcaggcggct
     1801 gtcactcaca tcgacgttca gcaggtcttc caggttgtca tcaaaagtga cgacgacgcg
     1861 gtaattgtcg ccgcgagtaa ttgtgccctg aaaggcattt tcgatgctga cttcatcgaa
     1921 gtcgctgagg tcgaactcgc gtgtgaccgt gttgccagag ccggtgacga agttcagatc
     1981 ggacaatgcc gagcagccga ccagaatcgc cacgctgagc atggccaggc caatgacaaa
     2041 cagctttttg agggacatga ctgactctcc ttgctacccc ctacaatacc ataccagcgc
     2101 cgcggctgaa gtaactttca ttataccttt tagaaggggg ataggcgggg ttggggtgaa
     2161 tggccgccaa acactggccc ctattctccc tttgcgggta tcatttaacc aacgaggtga
     2221 ccatgacagc cgacaacaag acgacattgc cggccacacg cctgggctgg ggcgaatcgc
     2281 gggcgctgag tctcgaccgc atcggctgga tgatccgctc ggcggccgcc cagggtgtgt
     2341 cccactataa ggtgctatgg ttcgacgtct tcttcgtggc cgaccctgat gttgtgcgcg
     2401 agctgatcgt caagcggccg cacctgctcc accgcgaccc gttcacgacc aacgtcctgc
     2461 gccgcatcat gggcaatggc gtcttcatcg ccgaggacga ggcctggcag cgacagcgca
     2521 agctcgtcgc cccggccttc cacgccatgc gcatccgcgc ctatgccgaa acgatggccg
     2581 ctttcacccg cgagatggcc ggccgctggc gcgatggcga ggtcgtgccc ctgggccggg
     2641 agctgaccgc cctgacgctg cgcatcatcg ccaagacgat gttcaatgtc gatttgttgc
     2701 cggaggcgga gcgcatcggc cggctgatgc aggcgctgtt ggaaacgggc gagaagcaac
     2761 tcgacgccgc ctacccgctg cccaactggg tgccgacgcc gaccaaccgc cgccagaacg
     2821 ccgccctgcg cgagttacat gccctggtgc gcgacattat ccgcgcgcgg caggcgagcg
     2881 gccgcgacga gggcgacctg ctgtccatgc tggtgcaggc ccgcgacgac gagggccggc
     2941 ccatgagcga ggcgcagatc atcgatgagt gcgttacgct gctgacggct ggacacgaga
     3001 cgacggccgt cgctctgatg tggacgtggc tgctactggg gcagcgcccc gatgtggccg
     3061 atgggctggt ggccgaggta gacagtgtgc tcggcggcgc gccggtgacg tttgagcttc
     3121 tggccgcgtt gcccatcacg gagagcatca tcaaggagac gttgcgcctc tacccgcccg
     3181 cgttcagctt cggccgcacg ccgcaggagg cgattgccct ggagatcaat gggcagagct
     3241 acttcttccg caaggggtcg agccttatca tgagcaccat tgccatgcac cgcttgccgg
     3301 agtacttccc ggagccggac gccttccggc cggagcgctg ggcgtctgga gaggccgcgc
     3361 caccgaagta cgcctacctg ccctttggcg gcgggccgcg tgtctgcctg ggcaatctct
     3421 tcgctatgat ggaggcccag gtcatcctgg cgacgatggc ccaacacgtg cgcctgtcgc
     3481 cagtggagcc cgggctgccg ccgtggtcgc cgctgatcac cctgcggccg aagggcgagg
     3541 tgagaatgcg cgtggcccgg cggacaccat agcaggccgt cgtgcaagtc tattcttgaa
     3601 ggggaggtga accatgtccg acagattggc cctccggttg ctgcgttgga gtccgcgcgt
     3661 gttggccatc ctctacgcca cgttcctcgg cctgttcgcc ctggacgcct ttggcagcgg
     3721 cagcaggctg gagcaggtcg tcggcttcct tatccatctg gccccggtct atgccgttgt
     3781 gctggcgctc atctttgcct ggcgctggcc gcggctgggc ggggtgctgt ttgtagcgct
     3841 ggcggtgggc ttcgtctttg cctttggctg gcgcgggctg ggtacggtgg tggtcatggc
     3901 tgggccgcca gtggccgcag gggtgttgtt cctggcgtcg ggctggggcg gacgggcgcg
     3961 gctgcgggcg cggttcta
//
LOCUS       DAOSMQ010000296         3954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_22994_length_3954_cov_4.058220, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000296 DAOSMQ010000000
VERSION     DAOSMQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22994_length_3954_cov_4.058220"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PK829_10105"
     CDS             complement(<1..392)
                     /locus_tag="PK829_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU41607.1"
                     /translation="MIADGQVVNEHVTQALAALNYDQLAEVQRHVAPTAFAPGSVVVR
                     QGEPGEHFYIITGGRAEVCVEQPDGRTLPVDSLRAGQYFGEMALLGRRPRRATVRAAA
                     DTPLEVVALDGATFSQLVESSADLRDEL"
     gene            complement(369..1046)
                     /locus_tag="PK829_10110"
                     /pseudo
     CDS             complement(369..1046)
                     /locus_tag="PK829_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1149..>1416)
                     /locus_tag="PK829_10115"
     CDS             complement(1149..>1416)
                     /locus_tag="PK829_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011760741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HOU41608.1"
                     /translation="FVGLVSSALVYVSEALGSVFLIAMLLPLLAAGARRLRDAGHSGW
                     WQLFLLAPVGGIVILAFLWALPPAGELPEENPNGVIAALAYRPS"
     assembly_gap    1417..1516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1517..2002)
                     /locus_tag="PK829_10120"
     CDS             complement(<1517..2002)
                     /locus_tag="PK829_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOU41609.1"
                     /translation="MPPPEALSSREWEVVEQLLEGKSNKQIAATLHITVRTVEFHLNN
                     VYNKLQVGSRTELILKLRQSTVVNQGELAENGDAPNSWATSWRDSVSRVGKELRMKIT
                     VDKDTYNEGGALTFQEAIIVCLTKYAEFDGRASRSEFWWFFLFVGLVSSALVYVSEAL
                     GS"
     gene            complement(2010..2351)
                     /locus_tag="PK829_10125"
     CDS             complement(2010..2351)
                     /locus_tag="PK829_10125"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOU41610.1"
                     /translation="MRWVMRSKIHKATVVQADVNYIGSITIDEDLMDRVGLWAHEKVL
                     VVSNTSGARLETYVIPGERGSGVINMNGAAAHLIKAGEEIIIIAFELTDRPIPARQIL
                     VDQNNHYVRDL"
     gene            complement(2469..3653)
                     /locus_tag="PK829_10130"
     CDS             complement(2469..3653)
                     /locus_tag="PK829_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOU41611.1"
                     /translation="MPRIRELGSYPSALPPGPRNAISDVAGVTVGHFTLISGEGAAGP
                     DWRPGHGPFRTGVTVIRPHGGNLYDDKVAAAVHTINGFGKVAGFEQVRELGTLETPIA
                     LTGTMNVPRVADALMTLAAEGSPHIGVGFAETGWRGYASVNPVVGETSDGYLSDLQAR
                     PIGLAEVRAALAAASADVGEGAVGAGTGTSCYGWKGGIGTASRLLPERAGGYTVGALV
                     QSNFGRAEELVICGVPVGRVLRPPADSANDRWPATGEEHPSPGPWNLAPGPSLPGGSI
                     MMILATDAPLDARGLGRLCRRAAFGLARTGSTGHGGSGDFVIAFSTTQRIPDRPDSPL
                     ALRPALYEQPLLDWFAAAVVESIEEAIYNSLLMAQTVAGRNGHIRHALPAAEVARLLN
                     RD"
     gene            complement(3666..>3954)
                     /locus_tag="PK829_10135"
     CDS             complement(3666..>3954)
                     /locus_tag="PK829_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU41612.1"
                     /translation="FPICRPDPLAALLRAAGLADVVVWLIDVATPFRDFDDYWRPFLS
                     GQGPAPGYVAALVAPRRAALRERLRATLPTAPDGSVSLIARAWAARGRPVG"
BASE COUNT          635 a         1267 c         1329 g          623 t
ORIGIN      
        1 tgcaactcgt cgcgcaggtc ggccgaggac tcaaccagtt ggctgaacgt cgcgccgtcg
       61 agggcgacga cctccagggg cgtatcggcc gcggcccgca ccgtcgcccg gcgcggccgg
      121 cggcccagca gagccatctc gccgaagtat tgcccggcgc gcagcgaatc gaccggcagg
      181 gtgcggccat cgggctgctc cacgcagacc tcggcgcggc cgccggtgat gatgtagaag
      241 tgctcgcccg gctcgccctg gcgcacgacg acgctgccgg gagcgaaggc cgtcggggcc
      301 acgtggcgct gcacctcggc caactggtcg tagttcagcg ccgccagggc ttgcgtcacg
      361 tgctcgttga ccacctggcc gtcggcgatc atgaccgagc ggctggtgcg ggcggcgcgg
      421 gcctcgtcgt gggtgaccat gatgatcgtc ttgccctcgg ccacgaggcg ttggaaaagg
      481 ctgaagatgc tctcggctgt gcgcgagtcg aggttgcccg tcggctcgtc ggcgatgagc
      541 aggccggggt cgttggccag ggcacgggcg atggcgacgc gctgctgctg gccgccggag
      601 acggccgcgg gcagcttgcg cgcttgccca tccatctcca ccagttccag caggcgcatg
      661 gcccggtcgc gccgctcggc agccgggtag agccggttga tgtccatcgg cagcatgacg
      721 ttttcgacca gcgtcagcgt cggcaatagc tggaagaact ggaagacgat gcccaggttc
      781 cgcccgcgcc acgcagccat gcgctcctcg tcgaaggtat gcaacggctc gccgccgacg
      841 acgatctcgc cggccgtcgg ccggtcgatg ccggtgatga ggttgatgag cgtggacttg
      901 ccgctgccgg atttgccgat gatggcgaca aactcgccgc ggttgacctg gagatcgatg
      961 cccttgaggg cggtgaagtc gcccgcgggc gtgcggaacg tcttgatgag gcggcgggcg
     1021 tcgacatagg gggcggcgtc aatcataggc cggtgattat aacgacaagg ccgggcgacg
     1081 cacctgttaa ggtgcgtcgc ccggccgatt gccgcatgaa gacgccaggc aggtatcgcc
     1141 gccggcggtc agctgggccg gtacgccagg gccgctatca caccgttggg gttttcctcg
     1201 ggtagctcgc cggccggcgg cagcgcccaa aggaaggcca atatgacgat cccgcccacc
     1261 ggggccagca ggaacaattg ccaccagccg ctgtgacccg cgtcgcgaag gcggcgcgcc
     1321 ccggcggcca acagcggcag gagcatggcg attagaaaca cgctaccgag ggcttcgctc
     1381 acgtacacga gcgccgagct gacaagcccc acgaacnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnngcta ccgagggctt cgctcacgta cacgagcgcc gagctgacaa
     1561 gccccacgaa caggaaaaac caccagaact ccgaccgcga cgctcgccca tcgaactccg
     1621 cgtacttggt gaggcacacg atgatggctt cctgaaaggt caacgcgcca ccttcgttgt
     1681 aggtgtcttt atccacagta atcttcattc gcaactcctt gccgacgcga gagaccgagt
     1741 ctctccagga tgtcgcccat gagttgggcg cgtctccatt ctcagcaagc tcaccctggt
     1801 tcacaactgt ggattgccgt agtttcagga tcagttccgt ccgggagccg acctggagct
     1861 tgttgtagac attattcagg tggaattcga cggtgcgaac agtaatatga agcgttgcgg
     1921 caatctgctt gttgcttttg ccctccagca gttgctcgac gacctcccat tcgcggctgc
     1981 tcaacgcctc gggtggtggc atggtatacc tatagatcgc ggacgtagtg gttgttctga
     2041 tcgacgagga tttgccgggc cgggatcggc cggtcggtca gttcgaaggc gatgatgatg
     2101 atctcctccc cggccttgat caggtgcgcc gccgcgccgt tcatgttgat gacgcccgac
     2161 ccgcgctcgc cggggatgac atacgtttcc agccgcgcgc cgctggtatt gctgacgaca
     2221 agcaccttct cgtgcgccca tagaccgacg cggtccatga ggtcttcgtc aatggtgata
     2281 ctgccgatgt aattgacgtc ggcctgcacg accgtcgcct tgtgaatctt ggaacgcatg
     2341 acccatctca tagcatgtag tatacgatga ggcgcgtatg aagttgctaa gaataggggc
     2401 taggttctag gggctaggtt ctagcgaaga gccttctttc ctagccccta gcccctagcc
     2461 cctaatccct agtccctgtt taggagacgc gcgacttcgg cggcggggag ggcgtggcgg
     2521 atgtggccgt tgcggccggc aaccgtttgg gccatgagca ggctgttgta gatggcttcc
     2581 tcgatgctct cgaccacggc cgcggcgaac cagtccagca acggctgctc atagagcgcc
     2641 ggccgcagcg ccagaggcga atcgggccgg tcggggatgc gctgggtggt gctgaaggcg
     2701 atgacgaaat cgccgctgcc gccgtggccg gtactgcccg ttcgcgccag gccgaaggcc
     2761 gcccgccggc acagtcgccc cagcccgcgg gcgtccagcg gcgcgtcggt ggccaggatc
     2821 atcatgatgg agccgccggg aagagagggg ccaggggcca ggttccaggg gccaggggaa
     2881 gggtgttctt ccccggtcgc tggccaccgg tcgttagcac tatccgcggg cggccgcagc
     2941 acccgaccga cgggaacgcc acagatgacc aactcctccg cccggccgaa gttgctctgc
     3001 accagcgcgc cgacagtgta gccgccagcc cgctctggca acaggcgcga cgccgtgccg
     3061 atgccaccct tccagccgta gcagctcgtg cccgtgcccg cgccgacggc cccctcgccc
     3121 acgtcggccg aggccgcggc caacgccgcc ctcacctcgg ccaggccgat tggccgcgcc
     3181 tggaggtcgc tcagatagcc gtcgctcgtc tcgccaacga cggggttgac actggcatag
     3241 ccacgccaac ccgtctccgc gaagccaacg ccgatgtggg ggctgccctc ggcggccagg
     3301 gtcatgagcg cgtcggccac gcgcggcacg ttcatcgtgc ccgtcagggc gatgggcgtt
     3361 tccagcgtgc ccagctcgcg cacctgctcg aagccggcca ctttgccgaa gccgttgatg
     3421 gtgtggacgg cggcggcaac cttgtcgtcg tagaggttgc cgccgtgggg ccggatgacg
     3481 gtcacgccgg tgcggaaagg gccgtggccg ggccgccagt cggggcccgc cgcgccctcc
     3541 ccgctgatga gcgtgaagtg gcccaccgtg acgccggcca cgtcgctgat ggcattgcgc
     3601 gggccgggtg gcagggcgct ggggtaggag ccaagctctc ggatgcgggg catgggtctg
     3661 ctcctctagc ccaccggccg gccccgcgcg gcccaggcgc gggcgatcag ggatacagag
     3721 ccgtccgggg cggtgggcag ggtcgcccgg aggcgctcgc gcagggccgc gcgccgcggc
     3781 gcaaccagcg cggcgacgta gccgggcgct ggcccctggc cgctcaggaa gggccgccag
     3841 tagtcatcga agtcgcggaa gggggtggcc acatcaataa gccacacgac gacatcggcc
     3901 aacccggcgg cccgcaacag cgcagccagc ggatcagggc ggcagatggg gaaa
//
LOCUS       DAOSMQ010000297         3950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23017_length_3950_cov_4.562773, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000297 DAOSMQ010000000
VERSION     DAOSMQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23017_length_3950_cov_4.562773"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2086)
                     /gene="ppk1"
                     /locus_tag="PK829_10140"
     CDS             complement(<1..2086)
                     /gene="ppk1"
                     /locus_tag="PK829_10140"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOU41613.1"
                     /translation="MIEAPPPPETVQDKPSKRRPAAEKPPTIDLRDPSLYINRELSNI
                     EFNRRVLHETQAEHPLLERAKFLAIFQSNMDEFFMVRVSGLKQQLRLGITAAQADGLL
                     PREQLANVHRTLTQLFSEANAIWRDQLHPQLAEANVHVLGYEELSKGQRNKLRAYFER
                     EIFPVLTPLASDPSRPFPHISNLSLNLAVQVRDPKTGDLHFARVKVPPALPRLVPLKR
                     FDPDQLSTPAVQKFVWAEQVIAANLDRLFPGMKIVASYPFRVTRNSDMQLQEEEADDL
                     LLMMEDNLRQRHFGSAVRLEIDDNMPADVRDILMSNLQVDSYDVYTTNGPLGLSSLWE
                     LHRLERPDLKDRPFAPRTPASLQNLGGSVFAVLRQRDLLLHHPYDSFTPVVNLLEAAA
                     DDPDVLAIKITLYRVGANPPVVQALKKARLNNKQVAAVVELKARFDEETNIHWAQELE
                     SVGVHVAYGLIGLKTHCKVTLIIRRERDGIRRYVHLSSGNYNSTTARLYTDIGFMSRD
                     EDLAADAGDLFNFLTGYSRQEEYRKFLVAPVTIRRGLIDAIKRETALGPKGRIIIKCN
                     SLVDGEMIRNLYRASQAGVKVDLIVRGICCLRPGIPGVSDNIQVLSIIGRFLEHSRIY
                     YFHNEGNPSLYMGSADLMPRNLDRRVEVLFPITDPEMQSHVVENILAICLRDTAKSHI
                     LQSDGRYIPRRYL"
     gene            2187..3914
                     /locus_tag="PK829_10145"
     CDS             2187..3914
                     /locus_tag="PK829_10145"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU41614.1"
                     /translation="MSGCAGKRVEGIAVGQPPTPAITGAAATAVSAAVAAPAQPDAAG
                     VLLPTPTLAAGLAPPASATPLAGAVVAPPPPATATPDPSQRGRYTLSAAPGVPEPLRQ
                     AAQHVATGNPELFTWVEGDPAADVRLTVADGEPLAQWVYAAAAPFATVADETTGAAVR
                     AGWASGASELGRLLLDTSTAAALEAAWGAPTGGESVAEDFLGRVWAARPSWIIVPFEA
                     LRPEWKVLRVDGLSPLTHDFAPEGYPLTVPMTVTGDPEATAAFRLRWAGPTANRDPAR
                     LTRVAMSGVTALVRATAAQMEQRGILWPGEAVAPVFQAADIAHVSNEVSFAADCPYPD
                     AYGGTTFCSRDAYFELLKNLGIDVIELTGNHLNDWGREPLARSLDMYAAAGMQWYGGG
                     RDLADASRPLVIAHNGNRIAFVGCNPVGPAGDWALVDAAGSRPCGPDLTAQIAELRAA
                     GHVVIATLQYIEHYRYDPPPDQIAAFAELAAAGASAVSGSQGHHAQGFAFDNGTYIHY
                     GLGNLFFDQMDMLATRQSFVDTYVVYDGRLISVELWTGLIEDWARPRLMTAAERADVL
                     QTTFAASGW"
BASE COUNT          591 a         1264 c         1370 g          725 t
ORIGIN      
        1 ggaggtagcg gcgagggatg tagcggccgt cgctttgcag gatgtggctc ttggctgtgt
       61 cgcgcaggca gatggcgagg atgttctcga cgacgtggct ctgcatctcg gggtcggtga
      121 tggggaatag cacttctacc cgccggtcga ggttgcgcgg catcaggtcg gcgctgccca
      181 tatagaggct ggggttgccc tcgttgtgga agtaatagat gcgcgagtgt tccaggaagc
      241 gaccgatgat gctcagcacc tggatgttat cgctgacgcc gggaatgccc ggccgcagac
      301 agcaaatgcc gcggacgatg agatcgacct tgacgcctgc ctgcgaggcg cggtagaggt
      361 tgcggatcat ttcgccgtcc accagcgagt tgcacttgat gatgatacgt ccctttggcc
      421 ctagcgcggt ctcgcgcttg atggcgtcga tcagcccccg gcgaatggtg accggggcga
      481 caaggaactt gcgatactcc tcctggcgcg agtagccagt caggaagttg aacaggtcgc
      541 cggcatcggc ggccaggtct tcgtcgcggc tcatgaagcc gatgtcggtg taaaggcggg
      601 cggtggtgct gttgtagttg ccgctggaca ggtggacgta gcggcggatg ccgtcgcgct
      661 cgcgccggat gatgagggtc accttgcagt gggtcttcag gccgatgaga ccataggcca
      721 cgtggacgcc gacgctttcc agctcttgcg cccaatggat gttcgtctcc tcatcgaagc
      781 gggcctttag ctcgacgacg gccgcgacct gcttattgtt cagccgcgcc ttcttcaggg
      841 cctggacgac gggcgggttg gcgccaacgc ggtagagcgt gatcttgatg gccaacacat
      901 cggggtcgtc ggccgctgct tccagcaggt tgaccaccgg tgtgaagctg tcataggggt
      961 gatggagcag caggtcgcgc tggcgtagga cggcgaagac gctgccgccc aggttttgca
     1021 gactggccgg cgtgcgcggc gcgaagggcc ggtccttcag atcgggccgt tccagccggt
     1081 gcagttccca aaggctgctg agacccagcg ggccgttggt tgtgtagaca tcgtatgagt
     1141 ccacttgcag gttgctcatc aggatgtcgc gcacgtcggc gggcatgttg tcgtcgatct
     1201 ccaggcgcac ggcgctgcca aagtggcgct gccgcagatt gtcttccatc attagcagca
     1261 ggtcgtcggc ttcctcctcc tgtagctgca tatcgctgtt gcgcgtcacg cggaagggat
     1321 agctggcgac gatcttcatg ccgggaaaga gccggtcgag gttggcagcg atgacctgct
     1381 cggcccagac aaacttctgt acggccggcg tggagagttg gtcggggtcg aagcgcttta
     1441 gcggcaccag ccgtggtagc gctggcggca ccttgacgcg ggcgaagtgc aggtcgcccg
     1501 ttttcgggtc gcgcacctgc acggccaggt tcagactgag gttggagatg tgggggaacg
     1561 gccggctggg gtcggaggcc agcggcgtta gcacggggaa gatttcgcgc tcgaaatagg
     1621 cccgcagctt gttgcgctgc cccttgctca attcctcgta gccgagcacg tggacgttgg
     1681 cctcggccaa ctgagggtga agctggtcgc gccagatagc gttggcctcg gaaaacagct
     1741 gtgtcagcgt gcggtggacg ttggccagct gctcgcgcgg caacaggccg tcagcctgcg
     1801 cggccgttat gcccagccgc aattgctgct tcaggcccga cacacgcacc atgaagaact
     1861 catccatgtt gctttggaag atggccagga acttagcccg ctccagcaag gggtgctcgg
     1921 cctgcgtctc gtgcaggacg cgccggttga actcgatgtt gctcaactcg cggttgatgt
     1981 agagactggg gtcgcgcagg tcaatcgttg gtggcttctc cgccgcggga cggcgtttgg
     2041 atggtttgtc ctggacggtt tcgggcggcg ggggtgcttc tatcatggca tatcctcttg
     2101 ctaaccttta gcgtgccaac cggaactgcg ctactcttcg gctgatgcgc acatcgctat
     2161 tatacgttgt tctactggca ttgttaatga gtggttgtgc ggggaagcgg gtagagggca
     2221 tcgccgtcgg ccagcccccc acgccggcca tcaccggcgc ggcggcgacc gcggtgtcgg
     2281 cggccgtggc cgccccagcc cagccggacg ctgccggcgt gctcttgccg acgccgacgc
     2341 tggccgccgg cctcgcgccg ccagccagcg cgacgccact ggccggggcc gtcgtcgcgc
     2401 ccccgccgcc ggccacggcc acgcccgacc ccagccaacg cggccgctat acgctgagcg
     2461 ccgcgccggg cgtgccggag ccactgcgcc aggccgccca gcacgtcgcc acgggcaacc
     2521 cggagctgtt cacctgggtc gagggcgacc ccgccgccga cgtgcggctg accgtcgccg
     2581 acggcgagcc gctggcccag tgggtttacg ccgccgccgc gcccttcgcc accgtggccg
     2641 atgagacgac cggcgcggct gttcgcgccg gctgggcgtc gggcgcgtcg gagttgggcc
     2701 ggctgctgct ggacacgtcc acggccgccg cgctggaagc tgcctggggc gcgcccaccg
     2761 gcggcgagag cgtcgctgag gacttcctcg gccgcgtctg ggcggcgcgg ccatcgtgga
     2821 tcatcgtgcc cttcgaggcg ctgcggccag agtggaaagt cctgcgcgtc gatggcctgt
     2881 cgcctctgac ccatgacttc gccccagagg gttatccgct gaccgtgccg atgaccgtca
     2941 ctggcgaccc ggaggcgacg gctgccttcc ggctgcgctg ggccgggcca acggccaacc
     3001 gcgaccccgc ccggttgacg cgcgtggcca tgagcggcgt caccgctctc gtgcgggcca
     3061 cggccgcgca gatggaacag cgcggcatcc tctggccggg cgaggcagtg gcgcccgtgt
     3121 tccaggcggc cgacattgcc cacgtcagca acgaggtctc cttcgccgcc gactgccctt
     3181 accccgacgc ctatggcggc acgaccttct gctcgcgcga cgcctatttc gagttgctca
     3241 aaaatctggg cattgacgtg atcgagttga ccggcaacca cctcaatgac tgggggcgcg
     3301 aaccgctggc gcgctcgctg gacatgtacg ccgccgccgg gatgcagtgg tatggcggcg
     3361 ggcgcgacct ggccgacgct tcgcggccgc tggtcatcgc gcacaacggc aaccgtatcg
     3421 cgttcgtcgg ctgcaaccct gttggcccgg ccggcgactg ggcgctggta gacgccgccg
     3481 gctcgcgccc ctgcggtccc gacctgaccg cgcagatcgc cgagttgcgc gccgcgggtc
     3541 atgtcgttat cgccacgctg caatacatcg agcactatcg ttatgacccg ccgcccgacc
     3601 agatcgccgc cttcgcggaa ctggccgcgg ccggggcgtc ggccgtctcc ggcagccagg
     3661 gccaccacgc ccagggcttt gccttcgaca acggcaccta catccactac gggttgggca
     3721 accttttctt cgaccagatg gacatgctgg ccacgcggca gagctttgtc gatacctacg
     3781 tggtctacga cggccgcctg attagcgttg aattgtggac gggcctgatc gaggattggg
     3841 cgcgtccgcg gctgatgacc gccgcagagc gcgccgatgt gctgcaaact acctttgccg
     3901 ccagcggctg gtaatgtggc gcgattctcc cgaatcgccc tctgcgccgg
//
LOCUS       DAOSMQ010000298         3939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23101_length_3939_cov_4.298146, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000298 DAOSMQ010000000
VERSION     DAOSMQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23101_length_3939_cov_4.298146"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1559
                     /locus_tag="PK829_10150"
     CDS             219..1559
                     /locus_tag="PK829_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU41615.1"
                     /translation="MADQFTSGYALLIAVNENLVPQYALPTVARDAVALRDVLVHPER
                     CAYPADNVRLLMGADASRDGIRGGLAWLKERIVADGRANATAVVFYSGHGAVNSDDNS
                     YYLLPYDLRSPITDSLLRASDVAAAIEQVQPRRLLVVLDCCHAGGMNIKGDDLFAGEG
                     LRKSAAPAETRAIAALSQGQGRAVLSSSSAAESSYVRSDRQMSIFTYHVVEALTGHAQ
                     AEGATEVLVSDIMGHVSRAVPASAQAEYGASQTPVYQTSGENFPVALLLGGKGISKGM
                     ALPDPVAPLPPGGPVISTGGGAYFAGPVSAGGDVGVGQKTTGGDEIRGSKYVMSGNFQ
                     GAVLNIESQLTNVTQSILAAPLGGDQRADLNSLVAALAAELQRVPAGRAGEAEAVAAR
                     LTTVSAALAEGDGELADISARALERAADALGDARPGVPAAARQLTAAVRKSLGQ"
     gene            1585..2364
                     /locus_tag="PK829_10155"
     CDS             1585..2364
                     /locus_tag="PK829_10155"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41616.1"
                     /translation="MNDASRAFWADVQGKLNTYFSGEEIETAAFVLGIDYDSLRGGAK
                     PTKINALLAEAGRQGLLGQLLTWARAERPNVVWPDAAANLELPGGAAEEGGATVFNIN
                     TGGGAYFGGPVNAGGDVGIGQKNVAGDEIRGSKYVMSGDFRGAILNIESKLDNVTQTL
                     GAMPAAAPDQRAELARLVGELKTALSAVPPEAAADAAKLADRVAALAEEATAGAPDGD
                     FVRDLGETVKRAAGKVAGAVPGVMTLAAAIVELVAAIVGQN"
     gene            2611..>3939
                     /locus_tag="PK829_10160"
     CDS             2611..>3939
                     /locus_tag="PK829_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU41617.1"
                     /translation="MRQRQRALFVLVLIALALGASVYRGRAAGPPIVISEVLVGNAST
                     NLDPDYTNYSSWVELHNSDAAAVSLAGLRLVSLRQGHPPAQSYTLPNVSIPAGGRVLV
                     WYDKLTAGGLHTPYELDMDGGWLELRTAAGDVIDSVTLGRQQPDVSFGRDAGGNWAFF
                     DPPTPGAANTSPAFAAVAGSVAAAPAFSVPGGRYSGARAVELSTTEPGGIIRYTLDGS
                     RPTPASPAYSAPIAIHTTQVVRARTFASGRISSPAVTQTYLIDVAANLPVVSIATDPD
                     NMFSDRMGIFVEGKNGIVGCGKKANWNQPWERPASVEMYDTGGARLFGQDAGIEIFGN
                     CSRNLKMKSFELKARKEYGDNDFSAPLFADRQLSSYRRLVLRTGGQDTANTLLRDALG
                     SQMLSGRMDIERGAWRPAIVYINGQFWGIYNLREKMDEDLVENHYGLDEET"
BASE COUNT          648 a         1384 c         1337 g          570 t
ORIGIN      
        1 ccgggggtct acttgacaca gtgtttcacg ggaaacaatt gttgtgatgc aacagcgggg
       61 caatggatcg aagcgggccg aagtgggtcg aagatcaccc cttgagcgcc tgcccatcac
      121 cagcggcggc aaatttttac atgccgcggg tctatccctc ccggccactt ggtgtataat
      181 gccccatcga aggccgcccg cggccgcgga ggaaacacat ggccgaccag ttcaccagtg
      241 gctacgccct gctcatcgcc gtcaacgaaa acctcgtccc ccagtacgcc ctgccgaccg
      301 tggcccgcga tgccgtggcc ttgcgcgacg tgctcgtcca cccggagcgc tgcgcctacc
      361 cggccgacaa cgtgcgcctg ctgatgggcg ccgacgcctc gcgcgacggc atccgcggcg
      421 ggctggcctg gctgaaggag cgcatcgtcg ccgacggccg cgccaacgcc acggccgtcg
      481 tcttctattc cggccacggc gcggtcaaca gcgacgacaa cagctactac ctgctgccct
      541 acgacctgcg ctcgcccatc actgactcgc tcctgcgggc cagcgacgtg gccgcggcca
      601 tcgaacaggt gcagccgcgc cgcctgctcg ttgtgctcga ctgctgccac gccgggggta
      661 tgaacatcaa gggcgacgac ctgttcgccg gggagggcct gcgcaagtcg gccgcgccgg
      721 ccgaaacccg cgccatcgcc gccctgtcgc aggggcaggg gcgggcggtg ctcagctcct
      781 cgtcggccgc cgaaagctcc tacgtccgct ccgaccgcca gatgagcatc ttcacctacc
      841 acgtcgtcga agccctgacc ggccacgccc aggccgaggg ggccaccgag gtgctcgtca
      901 gcgacatcat gggccacgtc tcgcgcgccg tgcccgccag cgcccaggcg gagtatggcg
      961 cgtcgcagac gcccgtctac cagaccagcg gcgagaactt ccccgtggcc ctgctgctgg
     1021 gtgggaaggg catcagcaag ggcatggctt tgcccgaccc tgtcgcccca ctgccgccgg
     1081 gcgggccggt catcagcacc ggcggcgggg cctatttcgc cgggccggtg tcggccggcg
     1141 gcgacgtggg cgtcggccag aagacgaccg gcggcgacga gattcgcggc agcaagtacg
     1201 tcatgtcggg caacttccag ggcgcggtgc tcaacatcga gtcgcaattg accaacgtca
     1261 cccagtcgat cctggccgcg ccgctgggcg gcgaccagcg cgccgatctg aatagcctgg
     1321 tggcggcgct ggcggcggag ttgcagcgcg tgccggccgg ccgggcgggc gaggccgagg
     1381 cggtcgcggc gcggctgacc acggtgtcgg ccgccctggc cgagggcgac ggcgaactgg
     1441 ccgacatcag cgcccgggcg ctggagcggg ccgccgacgc gctgggcgac gcccgtcccg
     1501 gcgtgcccgc cgccgcccgc cagcttaccg ccgccgtgcg taaatcgctg ggccagtagg
     1561 ctgaccagcc aaccggaggg agacatgaac gacgccagcc gggccttctg ggccgacgtg
     1621 cagggtaagc tgaacaccta cttcagcggc gaggagatcg agacggccgc gtttgtgctg
     1681 ggcatcgact acgactcgct gcgcggcggg gccaagccga ccaagatcaa cgccctgctg
     1741 gccgaggccg gccgccaggg gctgctgggc caactgctga cctgggcgcg ggccgagcgg
     1801 ccgaacgtcg tctggcccga cgccgcggcc aacctggagt tgcccggcgg cgcggccgag
     1861 gagggtgggg cgaccgtctt caacatcaac actggcggcg gggcctactt cggcgggccg
     1921 gtcaatgccg gcggcgacgt cggcatcggc cagaagaacg tcgccggcga cgagattcgc
     1981 ggcagcaagt acgtcatgtc cggcgacttc cgcggggcca tcctcaacat cgaatcgaag
     2041 ctcgacaacg tgacccagac gctgggggcc atgcccgcgg ccgcgcccga ccagcgcgcc
     2101 gaactggccc ggctggtggg cgaactgaag acggccctga gcgccgtgcc gccggaggcc
     2161 gccgccgacg ccgccaaact ggccgaccgc gtcgccgctc tggccgaaga ggccaccgct
     2221 ggcgcgcccg acggcgactt cgtccgcgac ctgggcgaga cggtcaagcg ggccgctggc
     2281 aaggtcgccg gggccgtgcc cggggtgatg actctggccg cggccatcgt cgaactggtg
     2341 gccgccatcg tcggccagaa ctgaggccac cattgcaatc ggggccgggc tgtcagggca
     2401 cgcccggccg cgccgcgcgt catagggcgc gggccaatac cgatacccgc cgcgcctggt
     2461 gatagggcaa aactcctaaa caagccagga cgtaagaaag attgaaagcg tcactcctta
     2521 tactagcgct gtcatcaccg tgttgccggt tacggtctat cattgcacgg ggcgcaatca
     2581 acgaacgccc gcgccagttg gggacgaacc atgcgtcagc gacagcgagc cttatttgtc
     2641 ctggtactga ttgcccttgc tctgggggcc agcgtctatc gcgggcgagc cgccgggccg
     2701 cccatcgtca tcagcgaggt gctggtgggc aacgcctcca ccaacctcga ccccgactac
     2761 accaactatt cctcctgggt tgaactgcac aacagcgacg ccgcggccgt cagcctggcc
     2821 ggcctgcgtc tcgtgtcgct gcgccagggg catccaccgg cgcaaagcta cacgctgccg
     2881 aacgtgtcca ttccggccgg cggccgcgtg ctggtctggt atgacaagtt gactgcgggc
     2941 gggctgcata cgccctacga actggacatg gacggcggct ggctcgagtt gcgcaccgcc
     3001 gccggcgacg tgatcgacag cgtgaccctg ggccggcagc agccggacgt gtcctttggc
     3061 cgcgacgccg gcggcaattg ggctttcttc gacccgccca cgcccggcgc ggccaatacg
     3121 tcgccggcgt ttgccgccgt cgccgggtcg gttgccgccg cgccggcctt ctccgtgccc
     3181 ggcgggcgct atagcggcgc gcgcgcagtc gaattgagca ccaccgagcc gggcggcatc
     3241 atccgctaca ccctcgatgg ctccaggccc acgcccgcct ccccggccta cagcgcgcca
     3301 atcgccatcc acaccactca ggtcgtgcgc gcccgcactt tcgccagcgg gcgcatcagc
     3361 agcccggccg tcacccagac ctatctgatc gatgtggccg ccaatctgcc ggttgtgtcc
     3421 atcgccactg acccggacaa catgttcagc gatcggatgg gcatcttcgt ggagggcaag
     3481 aacggcatcg ttggctgtgg caagaaggcc aactggaacc agccgtggga gcggccggcc
     3541 agtgtggaga tgtacgacac cggtggcgcg cgcctgtttg gccaggatgc cggcatcgag
     3601 atcttcggca actgctcgcg caacctgaag atgaagtcgt ttgaactgaa ggcgcgcaag
     3661 gagtacggcg acaacgactt cagtgctccc ctgtttgccg accggcagct gtcgtcctac
     3721 cggcgtctag tgctgcgcac gggcggccag gacacagcca acacgctgct gcgcgacgcg
     3781 ctgggcagcc agatgctcag cgggcggatg gacatcgagc ggggggcgtg gcggccggcc
     3841 atcgtctaca tcaacggcca gttctggggc atttataacc tgcgcgagaa gatggacgaa
     3901 gacctcgttg aaaaccacta tggcctggac gaggagaca
//
LOCUS       DAOSMQ010000299         3935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23124_length_3935_cov_5.369072, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000299 DAOSMQ010000000
VERSION     DAOSMQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23124_length_3935_cov_5.369072"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..695
                     /locus_tag="PK829_10165"
     CDS             78..695
                     /locus_tag="PK829_10165"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOU41618.1"
                     /translation="MPSTLFALVGLFAAMLLNRAADCWLNSTPLACGLTRHPRRAAAV
                     TVALPLLAVLAGRRAGSDGAALTITLFAAVLLLLAVVDWEQRRLPNAVVLPALAAAVA
                     LSADPLVAGLAAVAAFVVFLALYALGRRLYGFEALGMGDVKLAALVAAVAGPAAAAAL
                     LGGVLLAGGGAAVGLLSGRVQRGAALPYGSFLAVAGLVVRLWGLR"
     gene            706..1581
                     /locus_tag="PK829_10170"
     CDS             706..1581
                     /locus_tag="PK829_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41619.1"
                     /translation="MSAIVIRDLSTQEEYAAAEAIQRMTWGMSDLDIIPGHALHAMQH
                     SGSALLGAFDGKELVGFVFGILATHEIPGRIDPVAAARLKMYSVIAGVLPAYQGQDVG
                     YRLKMAQRDFCLRIGVRLVTWTYDPLESLNARFNIGKLGAVCRHYLRHFHGEMSGRNA
                     GLQTDRFDVEWWVTSHRAEARATRKWRPLRLEGLLAGGALLVNEASFNAAGLPVPPLN
                     YISQPSNLMLVEIPADFQAIKAADLDLARQWRAHTRAIFEGMFDSGFLVTDFVAREDE
                     AGRPRSYYLLTHGDA"
     gene            1584..2708
                     /gene="menC"
                     /locus_tag="PK829_10175"
     CDS             1584..2708
                     /gene="menC"
                     /locus_tag="PK829_10175"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOU41620.1"
                     /translation="MNIERIDLYHITQRLVSPFVTSFGPQQQRDCLLVAIHADGLTGW
                     GECVATNDPGYSYETVGTAWHVLSDFLIPALAGRSLDGPEALAPSLAFVRGHPLAKAA
                     LDQAVWDLAAQREGLSFAALLARPYAEGPRARVKVGVSIGIQPSIERTVEVIGAYVAA
                     GYGRIKLKIKPGHDVALARAARAAFPDLPIMLDANSAFRLEDAPTFQAMDDLNLLMLE
                     QPLGYEDIYDHSRLRPLIQTPLCLDESIHSADHARYALAIGACDIINVKPSRVAGWTE
                     ARRIHDLCRAADMSLWVGGMLETGVGRAAQLALAALPGFTLPGDISATDRYYERDITA
                     PFTLNREDSTLTVPDGPGLGVAIDFDQLAAVTLRRESFAG"
     gene            2736..3572
                     /gene="proC"
                     /locus_tag="PK829_10180"
     CDS             2736..3572
                     /gene="proC"
                     /locus_tag="PK829_10180"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOU41621.1"
                     /translation="MFTDQNIAFIGSGIMGEAMIRGLIVRNILPAGQIYAADPMPDRL
                     ADLAGRYGIHVTGNNAEAAEAAHIIVLSVKPQNLAEVMPGIRGHLRRQDLLLSILAGT
                     PIRKLADGVAHAAVVRAMPNTPAQIGQGMTVWTATPEVTEMQRAQAQAILGALGVELF
                     VNDEGYLDMATALSGTGPAYIFMFMEALIDAGVHLGFSRRVSEQLIFQTMRGSLEYAA
                     QSGKHVAELRNQVTSPGGTTAAALYHLEKGGLRTVLSRGIWAAYQRSVELGQGEKVKG
                     PA"
     gene            3588..>3935
                     /locus_tag="PK829_10185"
     CDS             3588..>3935
                     /locus_tag="PK829_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41622.1"
                     /translation="MDNDLTSTPAVVLQLDGRWQRFSEATRIIVARTAGEVVPALRAV
                     EAAVEGEGLFAAGYLAYEAAAAFGLSVHPPAEDSPPLLWFGLFRRREEVRSPSLREGL
                     GEGAAEDPLPNPLP"
BASE COUNT          575 a         1383 c         1319 g          658 t
ORIGIN      
        1 cattggcggg aatgatagcg caaccgcccg gcaagtaaag gatgcgaccc gcgcgcagac
       61 cgctataatc cgtcgccatg ccaagcacgc tcttcgccct ggtgggtctc ttcgcggcca
      121 tgctgctcaa ccgcgccgct gactgctggc tcaactccac gccgctggcc tgtggcctga
      181 cgcgccaccc acgacgggcc gcggccgtga cggtcgccct gccgctgctg gccgtcctgg
      241 ccgggcggcg ggctggcagc gacggcgcgg cgctgacgat cacgctattc gccgccgtac
      301 tgctgctgct ggctgtcgtc gattgggagc agcgccgcct gcccaacgcc gttgtcttgc
      361 cggcgctggc cgcggccgtg gccctcagcg ccgatccgtt ggtggccggg ctggccgcgg
      421 tggccgcctt cgtcgtcttt ctggctctct acgcgctggg gcggcggctc tatggttttg
      481 aggcgttggg catgggcgac gtcaagttgg ccgcgctggt ggccgctgtc gccgggccgg
      541 ccgccgcggc cgcgcttctg gggggcgtgt tgctggccgg cggcggcgcg gccgtggggc
      601 tgctctccgg tcgtgtccag cgcggggcgg cattgcccta cggcagcttc ctggccgtgg
      661 ccggtctcgt cgtccggcta tggggtctga ggtagaatca tccgaatgtc cgccattgtc
      721 atccgcgact tgtccaccca ggaagaatac gccgccgccg aggccatcca gcgcatgacc
      781 tggggtatga gcgatctcga tattattccc ggccacgccc tccacgccat gcagcacagc
      841 ggctcggccc tgctgggcgc gttcgacggg aaggagttgg tcggcttcgt cttcggcatc
      901 ctggccacac acgaaatccc cggccgcatc gatcccgtgg ccgcggctcg cctgaagatg
      961 tactcggtca tcgccggggt gctgccggcc tatcaggggc aggatgtcgg ctaccggctg
     1021 aagatggccc agcgcgactt ctgcctgcgc atcggcgtgc ggctggtcac ctggacctat
     1081 gacccgctgg agagccttaa cgcccgcttc aacattggca aactgggcgc ggtctgccgc
     1141 cactatctgc gccacttcca cggcgaaatg agcggacgca acgccggcct ccagaccgac
     1201 cgcttcgacg tggagtggtg ggtgaccagc caccgggccg aggcgcgggc cacgcgcaaa
     1261 tggcggccgc tgcgactgga ggggctgctg gccggcgggg ccttgctggt caatgaggcc
     1321 agcttcaacg ccgccggtct gcccgtgccc ccgctcaact acatcagcca accgagcaat
     1381 ctcatgctcg tcgaaatccc ggccgacttc caggccatca aggcggctga tctcgacctg
     1441 gcccggcagt ggcgcgccca cacgcgtgcc atcttcgagg gcatgttcga ctccggcttc
     1501 ctggtgaccg actttgtggc ccgcgaggac gaggccggcc gcccacgcag ttactacctt
     1561 ctgacccacg gggacgccta gccatgaaca tcgagcgcat cgacctctat cacatcaccc
     1621 agcgactggt cagccccttt gtgaccagct tcggcccaca gcagcagcgc gactgcctgc
     1681 ttgtggccat ccacgccgac gggctgaccg gctggggcga gtgcgtagcc accaatgacc
     1741 ccggctactc ctacgagaca gtcggcacgg cctggcacgt tctgagcgat ttcctcatcc
     1801 cggcgctggc cggtcgctcg ctggacggcc cggaagcgct tgccccttcg ctggctttcg
     1861 tgcgcggcca cccgctggcc aaagcggccc tcgaccaggc cgtctgggac ctggccgccc
     1921 aacgggaggg cctgtcgttt gccgccctgc tggcccggcc ctacgccgag gggccacggg
     1981 cgcgcgtcaa ggtcggcgtc agcatcggca tccagcccag tattgagcgc acggttgagg
     2041 tcattggcgc gtacgtcgcc gccggctacg ggcgcatcaa gctaaagatc aagcccggcc
     2101 acgatgtggc cctggcccgc gccgctcgcg ccgccttccc cgacctgccg atcatgcttg
     2161 acgccaactc cgccttccgg ctggaggacg cgcccacgtt ccaggcgatg gatgacctga
     2221 acctgctgat gctggagcag ccgctgggct acgaggatat ttacgaccac agccggctgc
     2281 ggccgctcat ccagacgccg ctatgcctgg acgaaagcat ccactcagcc gatcatgctc
     2341 gctacgccct ggccattggc gcgtgcgata ttatcaatgt caagccgtcg cgcgtggccg
     2401 gctggacgga ggcgcggcgc atccacgacc tctgccgggc ggcggacatg tccttgtggg
     2461 ttggtgggat gctggaaacg ggcgtcggcc gcgcggcgca actggccctg gcggcgctgc
     2521 ccggcttcac cctgcccggc gacatctcgg ccaccgaccg ctactatgag cgcgacatca
     2581 ccgccccctt caccctgaat cgcgaggaca gcacgctcac cgtgcccgac gggccggggt
     2641 tgggcgtggc catcgacttc gaccaactgg cggccgtgac tttgcgccgc gaatcgttcg
     2701 ccggctaagg gccgagcaca ggagaggcaa cgacgatgtt taccgaccag aatatcgcct
     2761 tcatcggcag cggcattatg ggcgaggcga tgatccgcgg cctgatcgtc cgtaacatcc
     2821 tgcccgcggg gcagatctac gccgccgacc ccatgcctga ccggctggcc gacctggccg
     2881 ggcgctatgg catccatgtg accggcaaca acgccgaggc ggccgaggcg gcccacatca
     2941 ttgtcctgtc ggtcaagccg cagaacctgg ccgaggtcat gcccggcatt cgcggccacc
     3001 tgcgccgcca ggacttgctg ctgtcgattc tcgccggcac gcccatccgc aagctggccg
     3061 atggcgtggc ccacgccgcc gtcgtccggg cgatgcccaa tacgccggcg cagatcggcc
     3121 agggcatgac cgtctggacg gcgacgccgg aagtgacgga gatgcagcgc gcccaggccc
     3181 aggccatcct cggcgcgttg ggcgtggagt tgtttgtcaa cgacgagggc tatctggaca
     3241 tggcgacggc cctcagcggc accggcccgg cctacatttt catgttcatg gaagcgctta
     3301 tcgacgccgg cgtgcacctg ggtttttcgc ggcgggtgtc ggagcaactc attttccaga
     3361 cgatgcgcgg ctcgttggag tacgctgccc agtcgggcaa gcacgtggcc gaattgcgca
     3421 accaggtcac ctcgcccggc ggcacaacgg cggcggcgct ctaccatctg gagaaaggcg
     3481 gcctgcgcac cgtcctctcg cgcggcatct gggccgccta ccagcgttcg gtcgagttgg
     3541 ggcaggggga gaaggtcaag gggccggcct gagcaccctg ccggcccatg gacaacgacc
     3601 tgaccagcac gccggcggtc gtcctgcaat tggacggccg ctggcagcgc ttcagcgagg
     3661 ccacacggat catcgttgcc cggacggcgg gagaggtggt tccggcgctg cgggcggtcg
     3721 aggcggccgt cgagggggag ggactgttcg ccgccggcta tctggcctat gaggctgccg
     3781 cggccttcgg gctgagtgtg cacccgcccg cggaggacag tccgccgctg ctgtggtttg
     3841 gcctcttccg ccggcgcgag gaagtccgat ccccctccct ccgggagggg ttaggggagg
     3901 gcgcggcgga agaccctctc cccaaccctc tccca
//
LOCUS       DAOSMQ010000300         3929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23171_length_3929_cov_4.096541, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000300 DAOSMQ010000000
VERSION     DAOSMQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23171_length_3929_cov_4.096541"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PK829_10190"
     CDS             <1..888
                     /locus_tag="PK829_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41623.1"
                     /translation="LAVRLDGAYLGQLLDFYSGRRDLRRGLIRVLHSLSFIDDPTRIL
                     RAVRLEQRLGFAIEPGTAELIGDALPMLDRVSGERIRNEIELALAEANPVRVMQRLDA
                     LGVLAHLAPGLGWRAETAAVFERVEAMADDPIWGETFRGGPAAFYYFAVWLAPFAPPV
                     PETVGRRLRVRKTTLHDLLGLGQLLPALAALPDNAPPSAVVAALEPYTPRVWLTARLL
                     TQDPRRVAWLDRVMTTWRHVQPALTGDDLRALGLPPGPVYTRILNRLLAARLDGEVTD
                     EAGERDLVARLLAREPLGD"
     gene            complement(885..3248)
                     /locus_tag="PK829_10195"
     CDS             complement(885..3248)
                     /locus_tag="PK829_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU41624.1"
                     /translation="MNDEPLRTTTPTIDIYIKLAQYPILADRVRARMRAELFDRGIVN
                     PELFEREVTELAQASQRREGLSEPFFEEAADAWQMRLERTRDTHTDAVFANHVGLARL
                     ESLIDEVLSSRPAPSDTMPISFNPEIAPWEMLFQQGEIYERMPPEELARHHHHLEEIK
                     VVLIKRLMSDQLPFIGVAKHVLAINDLRRIHQSRIGTGKIGGKAAGMYLAWRSLQPVA
                     AGLSADLAGRAAIHVPDSAFIGTDVIYEFLLMNRLEHYMNQKYRPVQEIRDEYPDIVA
                     AFMGSELPEYVVAEVSNLVNAFGDNPIVVRSSSLLEDNFGFAFAGKYDSFYCGNQGTP
                     AENLRAVLDAIRLVYASALNPDALLYRRKHNLIDYDERLAILIQRVTGHWHGNAFYPD
                     LGGVAFSQNPFAWAPEIRADQGFLRLVTGMSLRAVERVSQDYPRLVALSHPWLRPENT
                     LDDKRRFSQRFMAVIDRATERLATMPVREGLADNGPLLTLVASRDTGNDLAPVPPRTS
                     FAPDEHFVLTFEELTRDTAFIDLMRRVLAQLELVYGNPMNLEFAVNIDWSETPAEDAR
                     VPSPFYHLHILECRPLYRRDRDSDIPDLTALRDNHRLFAMPTLLPSVTVGNIEYLVFV
                     DPDSYYSLPDGPERRRVADTVTALNDLLPAGRFGIVGPGRWGNLDSRHSVPVTYSDIC
                     NSKLLVEISPAYTPPPELAYGTDFYEDVVEAGIVVVGVQPGQPGSEMDWALLRHSPNR
                     LAAFVPSSADLADIVRVIDLRGVAGSSLRLVIDSDANEAVACFDIPG"
     gene            complement(3245..>3929)
                     /locus_tag="PK829_10200"
     CDS             complement(3245..>3929)
                     /locus_tag="PK829_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41625.1"
                     /translation="DIVPNYPHPEYRLHLLQCRPLSQRAEGGPVTIPRDIPPGDILFT
                     SHHLVPDGRANDIRYIVYVDPQRYYTIPDVTVRHELARAIGRLNKRLEDERFILMGPG
                     RWGSTNIELGVHVTYADIFNTKVLIEMAVAHNGHMPELSYGTHFFQDLVEAGIHSLAL
                     HLKPGTGGSEFNTPFFANAPNALAELLPSEAALAGVLKVIDLDALPGGRRLQVLMDGH
                     HDEAIGYLT"
BASE COUNT          642 a         1188 c         1409 g          690 t
ORIGIN      
        1 ctggcggtgc gccttgacgg cgcgtatctg ggccaattgc tcgactttta tagcggacgg
       61 cgcgacttgc ggcgcggcct tatccgcgtc cttcatagcc tgagcttcat cgacgacccg
      121 acgcgcatct tacgggccgt gcggctggag cagcggctgg gcttcgcgat cgagccgggc
      181 acagccgaac tcatcggcga cgccctgccc atgctcgacc gcgtcagtgg cgagcgtatc
      241 cgcaatgaga tcgagctggc cctggccgag gcgaacccgg tgcgggtcat gcagcgcctg
      301 gacgcgctgg gggtgctggc ccatctcgcg ccgggtctgg gctggcgggc ggagacggcg
      361 gccgtgtttg agcgcgtgga ggctatggct gatgacccca tctggggcga gacgtttcgc
      421 ggcgggccgg cggcgttcta ctactttgcc gtctggttgg ccccgtttgc gccgcctgtg
      481 ccggagacgg tgggacgacg gctgcgcgtg cgcaagacca cgctgcacga cttgctgggg
      541 ctggggcaac tgctgcccgc actcgcggcc ctgcccgaca acgcgccgcc cagcgccgtt
      601 gtggccgctc tggagcccta cacgccgcgc gtctggctga ccgcgcgtct actgacgcaa
      661 gacccgcgtc gcgttgcctg gctcgaccgc gtcatgacga catggcggca cgtgcagccg
      721 gcgctgacgg gcgacgatct gcgcgccctc ggcttaccac ccggcccggt ctacacgcgc
      781 atcctcaatc gcctgttggc cgcccggctc gatggcgagg tgacggacga ggctggggag
      841 cgcgacctgg tggcgcggct gctggcccgc gagccactcg gcgattagcc gggtatgtcg
      901 aagcaggcca ccgcctcgtt ggcgtcgctg tcgatgacga ggcgcagcga actgccggcg
      961 acgccgcgca ggtcgataac gcgcacgatg tcggccaggt cggcgctgga aggcacgaac
     1021 gccgccagcc ggttgggtga gtggcgcagc agggcccagt ccatctcgct gccgggctgc
     1081 cccggctgga cgccgacgac gacaatgccc gcctcgacca cgtcctcgta gaagtcggtg
     1141 ccgtaggcca attccggcgg tggcgtgtag gcggggctga tctccaccag tagcttgctg
     1201 ttgcagatgt cgctataggt gacgggcacg ctgtgacggc tgtcgaggtt gccccagcgg
     1261 ccggggccga cgatgccgaa gcgccccgcc ggcagcagat cgttcagcgc cgtcaccgtg
     1321 tcggccacgc gccggcgctc tggcccgtcg gggaggctat agtagctgtc cgggtcaacg
     1381 aacaccagat actcgatgtt cccaacggtg accgagggca atagcgtcgg catggcgaag
     1441 aggcggtggt tgtcgcgcag cgcggtcaga tcggggatgt cgctgtcgcg gtcgcggcga
     1501 tagagcggcc ggcattccag gatgtggagg tgatagaagg gggagggaac gcgggcatcc
     1561 tcggccggcg tctccgacca gtctatgttg acggcgaact ccagattcat agggttgcca
     1621 tagaccaact ccaattgggc cagaacgcgg cgcatcagat cgatgaaggc cgtgtcgcgc
     1681 gtcaactcct cgaaagtgag gacgaagtgc tcgtccggcg cgaagctggt gcgcggcggc
     1741 actggagcga ggtcattgcc ggtgtcgcgc gaagccacca gcgtcagaag cgggccattg
     1801 tcggccagcc cctcgcgcac cggcatagtg gccagccgtt ccgtcgcgcg gtcgatgacg
     1861 gccatgaacc gttgcgaaaa gcgacgtttg tcgtcgagcg tgttctccgg ccgcagccag
     1921 gggtggctga gggcgacaag ccgagggtaa tcctgggaca cacgttcgac ggcgcgcagg
     1981 ctcatgccgg tgacaaggcg cagaaagccc tggtcggcgc gaatttcggg cgcccaggcg
     2041 aaggggtttt ggctgaaggc caccccaccc aggtcggggt agaaggcatt gccgtgccaa
     2101 tggccggtga cgcgctggat cagaatggcc aggcgctcat cgtagtcgat caggttgtgc
     2161 ttgcggcggt agagcagcgc gtccgggttg agggcgctgg cgtagacaag gcgaatggcg
     2221 tcgagcacgg cgcgcaggtt ctctgccggc gtgccctggt tgccacaata gaagctgtcg
     2281 tacttgcccg caaaggcgaa accgaagtta tcttccagca ggctggaaga gcgcacgacg
     2341 atggggttgt cgccgaaggc attgacgagg ttgctcacct cggcgacgac gtactcgggc
     2401 aactccgaac ccatgaacgc ggcaacgatg tcggggtatt cgtcgcgaat ctcctgcacc
     2461 ggccggtact tctggttcat gtagtgttcc agccggttca tgagcaggaa ttcgtaaatc
     2521 acgtctgtgc cgatgaaagc cgagtcgggc acgtgaatgg cggcccggcc ggcgaggtcg
     2581 gcgctcaggc cggcggcgac gggttgcagg ctacgccagg ccaggtacat gccggcggcc
     2641 ttgccgccga tcttgcctgt gccgatgcga ctctggtgga tgcggcgcag gtcattgatg
     2701 gccagcacgt gcttggccac accgataaac ggcagttggt cgctcatcag ccgcttgatg
     2761 agcacgacct tgatctcttc cagatgatgg tggtggcggg cgagttcctc cggcggcatc
     2821 cgttcgtaga tttcgccctg ttggaaaagc atctcccagg gggcaatctc cgggttgaag
     2881 gagatgggca tcgtgtcgga cggggcgggg cggctgctga gcacttcgtc gatcaggctc
     2941 tccagccggg ccaggccgac atggttggcg aagacggcgt cggtatgggt gtcgcgggtg
     3001 cgttccagcc gcatctgcca ggcgtcggcc gcttcctcaa aaaagggttc gctcagcccc
     3061 tcgcgccgct gcgaggcctg cgccagttcg gtgacctcgc gctcgaaaag ctccgggttc
     3121 acaatacccc ggtcgaacaa ctccgcgcgc atccgggcgc gcacgcgatc ggccaggatg
     3181 gggtattggg ccagcttgat gtagatgtcg atggtcggcg tcgtggtgcg caggggctcg
     3241 tcgttcatgt caaatagcct atggcttcat cgtgatggcc atccatgagc acctggagcc
     3301 gccggccgcc gggcagggca tcgagatcga tgaccttgag cacgccggcc agcgcggcct
     3361 cggatggcag cagttcggcc agagcattgg gcgcgttggc gaagaagggc gtgttgaact
     3421 cgctgccgcc cgtccccggc ttaaggtgga gggccagcga gtggataccc gcctcgacaa
     3481 ggtcctggaa gaagtgcgtg ccgtaggaca gctctggcat atggccgttg tgggccacgg
     3541 ccatctcgat caggactttg gtgttaaaga tgtcggcgta ggtgacgtgg acgcctaact
     3601 cgatgttggt gctgccccaa cggccaggcc ccatgagaat gaaacgctca tcttccagcc
     3661 gcttgttcag ccggccgatg gccctggcca gctcatgacg cacggtcacg tcggggatgg
     3721 tgtaatagcg ctggggatcg acgtagacga tgtagcggat gtcgttggcg cgcccgtcgg
     3781 gcacgaggtg gtgggaagtg aaaaggatat cccccggcgg gatgtcgcgc gggatggtca
     3841 ccgggccacc ttcggcgcgc tggctcagcg gccggcattg gagcaggtgc aaccggtatt
     3901 ccgggtgtgg gtagttgggc acgatatcg
//
LOCUS       DAOSMQ010000301         3923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23210_length_3923_cov_5.106256, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000301 DAOSMQ010000000
VERSION     DAOSMQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23210_length_3923_cov_5.106256"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK829_10205"
     CDS             <1..208
                     /locus_tag="PK829_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOU41626.1"
                     /translation="VIWTPGPMTGCPVLEGAAAVIECRVVQLIDIGGDHDLLVGEVVN
                     ALLLKPGDPHTSLTLPDLNWSYAG"
     gene            complement(225..1037)
                     /locus_tag="PK829_10210"
     CDS             complement(225..1037)
                     /locus_tag="PK829_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HOU41627.1"
                     /translation="MTKEEFEEEMEVMEKPRKRSRARPVLLGLAALLFVALAFLAVAV
                     AQLVRTVNNTAEGAAAAVDPITDRVRQLFVPATPVILPDPATIVRQINDLARLETASY
                     EMEKIVTAESEQDGLLGLLLGESMIFVAYGKVYAGVDFSRMTPADMVVVDPQTVRVHL
                     PEAEIFTDIPVLDNERSYVADRDTGLLTNADPALETQVRQAAESAIREAATSSDILGR
                     ANTNAQTYMRNLLGGLGFTNIEFTDTTPPPAAPYQQDVPKGYYLLPTATPAP"
     gene            1216..1500
                     /locus_tag="PK829_10215"
     CDS             1216..1500
                     /locus_tag="PK829_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOU41628.1"
                     /translation="MIVATCVISLDLSGLDSLKDKRSIVRSIIARLRREFNLSVAEVA
                     EMDVRTLGVIGLAGVGNDRRYLHGQMEKAVAWIVAQRPDVPIYDYSIEFV"
     gene            1504..3480
                     /locus_tag="PK829_10220"
     CDS             1504..3480
                     /locus_tag="PK829_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41629.1"
                     /translation="MRISLALLWLATLLLRPAAQAGDLTLSARAGFDGFYRNGATVPV
                     FVSARNDGAPLEGEIRVAVDDGAGGQNVFTAPIALPTQSDKRVALYVHVPGFSGSLKV
                     ELVSDERVVATATTDSLTLVSPDTLFYGVVSADPGALAFLETVTGGRAEADVAFLDLA
                     NLPDVSIAWRALDVLIFDDVDTSRLTAGQLAALRTWVENGGHLVVTGGPGGPKTAAAL
                     ADLLPVTVSGVASVAALPALEDFAGAPTTAPGPIVLTTSSLRRGEVLLEQDGRPLLAA
                     GNLGLGRVTFLALDPKLAPLAGWAGHDALWMTIAAATPTPGPWSNGIQDSYTANAAVS
                     AIPGLRLPSLAALLLFLLAYTLVIGPINFLVLRRLRRRELAWVTIPVLVLLFSAVTYF
                     TSFHTRGREAVLHTLTVAQGSIEAEQVRMQSAVGFYSPRRGRFSLTLPYDATAVPFAG
                     GAFGSVSGNVDAIVRAGDLTLRDIRNDTGQVTAFLVDSHAPRPALTGEVRLAAGGSAL
                     DVTLRNDAGATFENAVVVYGDQQVLVGDMAAGASREVSLPLTTPLGTSAGATPTPDPL
                     FPTGIVIPNPLINDPTFILGTPDYYTDADAYTRWQLLQSLYNYSAPVAGQSPAASVTL
                     AGWLSDSAQAIDVGDVAVTRSATTLVLLEIPLRP"
     gene            3477..>3923
                     /locus_tag="PK829_10225"
     CDS             3477..>3923
                     /locus_tag="PK829_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41630.1"
                     /translation="MTALIELHGLTKRYGDLTAIDDLTLDIEEGAVFGFIGPNGAGKT
                     TTMRILTTLLRPTAGTARIAGHSVSDDPRAVRRAIGYMPDFFGVYEDMKVWEYLDFFA
                     ACYDVPTARRAGMIDDLLALVDLEPKRDAFVDTLSRGMKQRLCLART"
BASE COUNT          604 a         1386 c         1250 g          683 t
ORIGIN      
        1 cgtcatctgg acgccgggac cgatgaccgg ctgccccgtc ctggagggcg cggcggcggt
       61 catcgagtgc cgtgtcgtcc aactcatcga catcggcggc gaccacgacc tgctcgtcgg
      121 cgaggtggtt aatgccctgc tgctgaagcc gggcgacccg cacacgtcgc tgacactgcc
      181 cgacctgaat tggagctacg ccggctaagg cgtagcgacg ccagttaggg cgcgggcgta
      241 gccgtcggca gcaggtaata gcccttgggc acgtcttgct ggtagggcgc ggccggcggc
      301 ggcgtcgtgt cggtgaattc gatgttagtg aaacccaacc cacccaacag attgcgcatg
      361 taggtctgcg cgttggtatt ggcccggccg aggatgtcgc tgcttgtggc cgcctcgcgg
      421 atggcgctct cggcggcctg gcgcacctgc gtctccagcg ccgggtcggc gttggtcagc
      481 aggccggtgt cgcggtcggc cacgtaggaa cgctcattat caagcacggg gatgtcagtg
      541 aaaatctcgg cctccggcag gtggacgcgc acggtctggg gatcgacgac gaccatgtcg
      601 gccggcgtca tgcggctgaa atcgacgccg gcatagacct tgccataggc tacgaagatc
      661 atcgactcgc ccaggagcag gcccagcagg ccgtcttgct ccgattcggc ggtgacgatc
      721 ttttccatct cgtaagaggc tgtctccagg cgggccaggt cgttgatctg gcggacaatg
      781 gtcgccggat cgggtaagat gaccggcgtg gccggcacaa acaactggcg gacgcggtcg
      841 gtgatgggat cgacagccgc cgcggctccc tcagccgtgt tgttgaccgt gcgcaccagc
      901 tgcgccacgg ccaccgccag gaaggccagg gccacaaaca acagcgccgc caaccccagg
      961 agaaccgggc gcgcccgcga gcgcttgcgc ggcttctcca tcacctccat ctcctcctca
     1021 aactcttctt tcgtcattcg ttcactcaac ctggtcgatg ggggcatcgc agcccctgac
     1081 atccatgact ataccctatc tgcatatgca gaaccttaac actctacgcc gggcgcgcgt
     1141 tcgggtcgcg acaggccgcc gccgctcacc gtccgctcac ttgccgccgg gcagcgaatg
     1201 gggacaatta cgttcatgat tgtcgccact tgtgtcatca gcctcgacct gagcggcctg
     1261 gattcactta aggataagcg tagcatcgtg cgctccatca tcgcccgctt gcggcgcgag
     1321 ttcaacctgt ccgtggccga ggtggccgag atggacgttc gcaccctggg cgttatcggc
     1381 ctggctggcg tcggcaacga ccggcgctac ctgcacggcc agatggagaa ggctgtggcc
     1441 tggatcgttg cccagcgtcc cgacgtgccc atctacgact acagcatcga attcgtctag
     1501 gccatgcgca tctctctggc acttctctgg ctggcgacgc tgctgctgcg cccggcggcc
     1561 caggctggcg acctgacgct cagcgcccgc gccggcttcg acggcttcta ccgcaacggg
     1621 gccaccgtgc ccgtctttgt ctccgcgcgc aacgacggcg cgccgctgga aggggagatt
     1681 cgcgtcgccg tagacgacgg cgcgggcggc cagaacgtct tcaccgcgcc catcgccttg
     1741 ccaacgcagt cggataagcg cgtggccctc tacgtccatg tgcccggctt ctccgggagt
     1801 ctcaaagtgg agcttgtcag cgacgagcgt gttgtggcca cagcgacaac cgactcactg
     1861 accctggtct cgcccgacac gctgttctac ggcgtcgtca gcgccgaccc cggcgcgctg
     1921 gcctttctag agacggtgac gggcggccgc gccgaggcag acgtggcctt cctcgatctg
     1981 gccaacctgc ccgacgtctc catcgcctgg cgcgcgctcg atgtcctcat cttcgatgac
     2041 gtcgacacca gccgcctgac ggccgggcaa ctggccgcgc tgcggacgtg ggtggagaat
     2101 ggcggccatc ttgtcgtcac cggcggcccc ggcggcccca agacggccgc tgccctggcc
     2161 gacctgctgc cggtgacggt cagcggcgtc gcgagcgtcg ccgcgctgcc cgccctggag
     2221 gacttcgccg gcgcgccgac gaccgcgccc ggccccattg ttctgaccac cagcagcctc
     2281 cgccgcggcg aggtgctgct ggagcaagac ggccggccgc tgctggctgc cggcaacctc
     2341 gggctggggc gcgtgacctt cctggcgctc gaccccaagc tggccccgct ggccggctgg
     2401 gctggccacg acgcgctctg gatgactatc gccgcggcga cacccacccc tggcccatgg
     2461 agcaacggca tccaggacag ctacacggcc aacgcggccg tgtcggccat tcccggcctg
     2521 cggctgccgt cgttggccgc gctgctcctc tttctgctcg cgtacacact ggtcattggg
     2581 ccgatcaact tccttgtgct gcgccggctg cggcggcgcg aactggcctg ggtgaccatc
     2641 cccgtgctgg tgctgctgtt cagcgccgtc acctacttca ccagcttcca cacccgcggc
     2701 cgcgaggctg tgctccacac cctgactgtg gcccagggtt ccattgaggc cgagcaggtg
     2761 cgtatgcaga gcgccgtggg gttctattcg cctcggcggg ggcgttttag cctgacattg
     2821 ccctacgacg ccacggctgt ccccttcgct ggcggcgctt tcggttctgt cagcggcaac
     2881 gtggatgcca tcgtgcgcgc cggcgacctg accctgcgcg acatccgtaa cgacaccggc
     2941 caggtgaccg ctttccttgt cgatagccat gccccgcggc ccgccctgac cggtgaggtg
     3001 cggctggccg ccggcggtag cgcgttggac gtgacgctgc gcaacgacgc cggagccacc
     3061 ttcgagaacg ccgtcgtcgt ctatggcgac cagcaggttc tcgtgggcga catggccgcg
     3121 ggcgcgtcgc gcgaagtatc gctgccgctg acaacccctc tgggaacctc cgcgggagcc
     3181 acgccgacgc ccgatccgct gtttccgaca ggcatcgtca tccctaaccc actcattaac
     3241 gatccgacat tcatcctggg cacgcccgac tactacaccg acgctgacgc ctacacacgc
     3301 tggcaattgc tccaatccct ctataactac tccgcgccag tggcggggca gtcgccagcg
     3361 gcctcagtga cgctggccgg ctggctgtcc gacagcgccc aggccatcga cgtgggcgac
     3421 gtcgcggtaa cacggtcggc cacaaccctg gttttgctgg agataccgct gcgcccatga
     3481 ccgccctgat tgaattgcac ggccttacca agcgctacgg cgacctcacc gccatcgacg
     3541 atctaacgct cgacattgag gaaggggccg tcttcggctt catcggcccc aacggcgcgg
     3601 gcaagacgac gaccatgcgc attctgacga cgctgctgcg gccgacagct ggcacggccc
     3661 gcatcgccgg ccactccgtc agcgacgacc cccgcgccgt ccgccgcgcc atcggctaca
     3721 tgcccgactt cttcggcgtc tacgaggaca tgaaggtctg ggaatacctg gacttcttcg
     3781 ccgcctgcta cgacgtgccc acggccaggc gggcgggcat gatcgacgac ctgctggcgc
     3841 tggtagatct ggagccgaag cgcgacgcct tcgtggacac gctcagccgc ggcatgaagc
     3901 agcgcctctg cctggcgcgg acg
//
LOCUS       DAOSMQ010000302         3871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23598_length_3871_cov_4.550052, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000302 DAOSMQ010000000
VERSION     DAOSMQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23598_length_3871_cov_4.550052"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PK829_10230"
     CDS             complement(<1..822)
                     /locus_tag="PK829_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41631.1"
                     /translation="MATGSPAPTPGNRSSRPADSLSDQTRAYGLRLLYRLGLAVAIGA
                     GMSLVVSLWAMATGIGLWRLGALGLLLAAGVVGLNSGRGEVADAGRRPFIVLLLAGYL
                     MVTLPTAFAGAMTGLVGLWVMAWFGVLVLSAGLLLGRVWALAMSAVGLGALLTALVPS
                     VGRADAPGNAGALIALAAIALGVFTFALNLVFEAIARARAQSQELQRSLWQTRATLEG
                     QVEERTRAQALAARVGRQLTRIHDLDTLLHEAVEMIRERFDLYHVQVYLLNPVAGE"
     gene            complement(809..2830)
                     /locus_tag="PK829_10235"
     CDS             complement(809..2830)
                     /locus_tag="PK829_10235"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOU41632.1"
                     /translation="MNIAGWQFTPFFVPVLGAAIFATALALFAWRRRDDTPGTWVFLV
                     LVSLTAWWSFTYALELATTDPGTMLLWVKMEWITIALLPLALLLFALVYTGYSRFVTL
                     RNVALLMILPLIIIALAWSTPSHTLLYANPGVVDNGSFIAFYAQRGPAFYVHVAYGYI
                     LLLVAIVLMARAWRQAEGEARKLAFLVLLGSLVAFFGNIAYQAALLLDLPLYVDLTVL
                     AFLFSIILFAWGWFRFRLADIVPELRELAPVPADADMANLAHNTQLRILNLVSLGLGL
                     LLFVALVPILTLLSREEVSRWPFMAAYVVLFFILVVVALWRSGPYGLRATGLATVYLG
                     LLLLDLRVNGLTPAAGLFMVTYAAFVAILFNRRAAILGLAIGVIGLLSTPPPEVTAPI
                     RDIYSLIYLLLSIFMTIALLLWGLFALRRDSQTLLRRSRELTRTLDVERAQLEQRVAE
                     RTRALETSATISRQLSTILDRAELVREVVEQLRVAFAYYHVHIFLWDEASGLLKMAGG
                     TGEAGQAMLVMGHAIAPDQGLVGRAFMTNTPLVVPDVSRDPYWLPNRLLPGTRSEIAV
                     PINYGDEILGVIDAQDSEVNGLGQQDSQLLQTIAGQLAVALRNARLLAQIQQQAEQAA
                     LINTINQKIAQTIDMDGAIRVALVELSRALEAGSAAVRLEADGGANGHG"
     gene            complement(2849..>3871)
                     /gene="gatB"
                     /locus_tag="PK829_10240"
     CDS             complement(2849..>3871)
                     /gene="gatB"
                     /locus_tag="PK829_10240"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOU41633.1"
                     /translation="DYNRSGVPLLEIVSEPDIRSGDEARAYATRIRQILRYLGVNSGD
                     MEKGVLRVEPNISLRPRGRQTFGTRTELKNLNSFRALADGTDYELARQAAVLDAGGEV
                     VQETRGWHDARRETFSQRSKEEAEDYRYFAEPDLPPLFIDAAWIEAARASLPELPQAK
                     EARYQAELGLSAGDAAALAEDCAVAAWFEAAVAAGGAPKTLANWILNDLFRLLNESGR
                     RVDDIPLAPAALVELLRLVDDGAVNLTSAREVLAEMVATGRGAAEIVAARGLTQISDE
                     AALLAVIEGLIADNPGPVANYLGGKTSLLGWFVGQVMRATRGQANPALANRLVAEQLA
                     ARGQTP"
BASE COUNT          725 a         1256 c         1335 g          555 t
ORIGIN      
        1 ttcgccggcc accggattga gcaagtacac ctggacgtgg tacaggtcga agcgctcgcg
       61 gatcatctcg acggcctcgt ggagcagcgt gtcgaggtcg tggatgcgcg tcaattgccg
      121 gccgacgcgg gcggccagcg cctgggcgcg ggtgcgttcc tcgacctgac cttccagggt
      181 cgctcgcgtc tgccagaggc tacgttgcaa ctcctggctc tgggcgcggg cgcgagcgat
      241 ggcctcgaag accaaattga gcgcgaaggt aaacacgccg agggcgatgg ccgccagggc
      301 gatgagagcg ccggcgttgc cgggagcgtc cgcgcggcca acgctgggca ctagcgccgt
      361 cagtagcgcc cccagcccga cggccgacat ggccagcgcc cagacgcggc caagaagcag
      421 cccggctgat agcaccagca caccgaacca ggccatcacc cacagaccga ccagcccggt
      481 catggccccg gcaaaggcgg tcggcagcgt aaccatcagg tagccagcca gcaacagaac
      541 gataaacggc cggcgacccg cgtctgccac ctcgccgcgc ccggaattca ggccgaccac
      601 cccggcggcc agcaaaagcc ccagcgcgcc cagccgccac aggccgatgc cggtcgccat
      661 tgcccacagg ctaactacga gcgacatgcc tgcgccgatg gccacggcca gccccaaccg
      721 gtagagcagc cgcagcccat aggcgcgcgt ctggtcgcta agagagtctg ccggccgcga
      781 cgaccggttg cccggcgtgg gcgcggggct acccgtggcc attagctcct ccgtcggcct
      841 ccaggcgcac tgcggcgctg cccgcctcca gagcgcgtga tagttcgacc agagcgacac
      901 ggatggcgcc atccatgtcg atggtctggg cgatcttctg gttgatcgtg ttgatgaggg
      961 cggcctgctc agcttgctgc tgaatctggg ccaggaggcg ggcgttgcgc agggcgacag
     1021 ccagttggcc ggcgatggtc tgtagcagtt gcgagtcctg ctggccgagg ccattgacct
     1081 cgctgtcctg ggcgtcgatg acgcccaaaa tctcgtcgcc atagttgata ggcacggcaa
     1141 tctccgagcg cgtgccgggc agtaggcggt tgggcagcca gtagggatcg cggctgacat
     1201 cgggcacgac gaggggggtg ttggtcatga aggcacggcc gaccagcccc tggtctgggg
     1261 cgatggcatg gcccatgact agcatggcct ggccggcctc gcccgtgccg ccggccatct
     1321 tcagcaggcc gctggcttcg tcccacagga aaatgtggac gtggtagtaa gcaaaggcca
     1381 cgcgcaactg ttcgacgacc tcacgcacca gttcggcgcg gtcgaggata gtagacaact
     1441 gccggctgat ggtggcgctg gtctccaggg cgcgagtgcg ctcggcgacg cgctgctcca
     1501 gctgggcacg ctcgacatcc agggtgcggg tcagttcccg cgagcgtcgc aacagcgtct
     1561 ggctgtcgcg acgcagggcg aaaaggcccc acagcaggag ggcgatggtc atgaagatgc
     1621 tgagaagcag gtagatcagc gagtagatgt cacgaatagg cgcggttact tccggtggcg
     1681 gcgtggacag caggccaatc acgccgatcg ccaggcccag gatggccgcc cgacggttga
     1741 agaggatggc gacgaaggcg gcgtaggtga ccatgaacag accagccgcc ggagtcaggc
     1801 cgttcacgcg caagtcgagg agaagcagcc ccaggtaaac cgtcgccagc cccgtcgccc
     1861 gcagcccata tggccccgac cgccacagcg ccacgaccac aagaatgaag aagaggacca
     1921 cgtaggccgc catgaatggc cagcgcgaca cctcctcacg cgacagaagg gttaggatgg
     1981 gcaccagagc cacgaacagc agcagcccca ggcccagcga gacgaggttg aggatgcgca
     2041 gttgggtgtt gtgggccaga ttggccatgt cggcgtcggc cggcaccggg gccagttctc
     2101 tcaactcggg cacgatgtcg gccagccgga accggaacca gccccaggca aacaggatga
     2161 tgctgaacag aaaggccagc accgtcaggt caacatagag cggcaggtcg agcagcaagg
     2221 cggcctgata ggcgatgttg ccgaagaaag ccaccagcga ccccagcaag acaaggaagg
     2281 ccagcttacg ggcctcacct tccgcttgcc gccaggcgcg ggccatcagc acaatggcca
     2341 ctaagagcag gatgtagcca taggcgacgt gaacgtaaaa ggccggcccg cgctgggcat
     2401 agaaagcgat gaagctgccg ttatccacga cacccggatt ggcatagagc agcgtgtgtg
     2461 agggcgttga ccaggccagg gcgatgatga tcagcggcag gatcatcagc aaggcaacgt
     2521 tgcgcaacgt gacaaagcga ctgtagccag tgtagaccag cgcgaacagg agcaaggcca
     2581 ggggcagcag agcgatggta atccactcca tcttcaccca caggagcatc gtgcccggat
     2641 cggttgtggc cagttccagg gcataggtga agctccacca ggccgtcagc gacaccaaca
     2701 caaggaagac ccaggtgccg ggcgtgtcgt cgcgccgccg ccaggcaaag agcgctagag
     2761 ccgtggcaaa gatggccgct cccagcacgg gaacgaagaa gggtgtgaat tgccagccgg
     2821 cgatgttcat gtcttgtcct gcgtccggtt acggcgtctg gccgcgtgcc gccaattgct
     2881 cggccaccag ccggttggcc agggccgggt tggcctggcc gcgcgtagcc cgcatcacct
     2941 ggccgacaaa ccagcccagc aggctcgtct tgccacccag gtagttggcc accgggccgg
     3001 ggttgtcggc gatgaggccc tcgatgacgg ccagcagtgc cgcctcgtcg gaaatttgcg
     3061 tcagaccgcg ggcggcgacg atctcggccg cgccgcgccc cgtggccacc atctcggcca
     3121 gcacctcgcg ggccgaagtc aggtttaccg cgccgtcgtc caccagccgc agcagttcca
     3181 ccagcgccgc gggagccagg gggatgtcgt cgacgcgccg gccgctctcg ttcagcagcc
     3241 ggaagaggtc attgaggatc cagttggcca gcgtcttggg cgcgccgccg gcggcaacgg
     3301 cggcctcgaa ccaggcggcc acggcgcaat cctcggccag cgcggcggcg tcgccggcgg
     3361 acaggcccag ctcggcctgg tagcgtgcct ccttggcttg cggcagttcg ggcaacgacg
     3421 cccgcgcggc ctcgatccag gcggcgtcaa tgaacagcgg cggcaagtct ggctcggcga
     3481 agtagcggta gtcctcggcc tcttccttgc tgcgctggct gaaggtctcg cggcgggcgt
     3541 cgtgccagcc gcgcgtctcc tggacaactt cgccgccggc gtcgagcacg gccgcctgtc
     3601 gtgccagctc gtaatcggtg ccatcggcca gggcgcggaa gctattgagg ttctttagct
     3661 cggtgcgcgt gccgaacgtc tggcggccgc gcggccggag gctgatgttc ggctccacgc
     3721 gcaggacgcc tttttccatg tcgccggagt tgacgcccag atagcgcagg atttggcgga
     3781 tgcgcgtggc gtaggcgcgg gcctcgtcgc cgctgcggat gtccggctcg gagacaatct
     3841 ccagcagggg cacgccggag cggttgtaat c
//
LOCUS       DAOSMQ010000303         3859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23701_length_3859_cov_3.620662, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000303 DAOSMQ010000000
VERSION     DAOSMQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23701_length_3859_cov_3.620662"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PK829_10245"
     CDS             <1..580
                     /locus_tag="PK829_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOU41634.1"
                     /translation="AGLSLEAIIEPGYFGRTGAEVLCLGAGGSAVATLLYLMNKPDPA
                     DRPRRFIVVNRSQGRLDHLQDMVARIGSDIAVEAICNADPRVNDEIMAFLPEHSLVIN
                     ATGMGKDSPGSPITDAGVFPRRGIAWEFNYRGELDFMHQALRQVDSRALRVEDGWIYF
                     VHGWSQVVAQVLHVDLTPEVFGRLERAAATVR"
     gene            604..1935
                     /locus_tag="PK829_10250"
     CDS             604..1935
                     /locus_tag="PK829_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41635.1"
                     /translation="MKAKLAPIYFDPGRDEGFDGQLARLRELLADDAEFLEPVALGRG
                     LPREADAAVFPQLLGEAYRRVGDFRRIDVPILVVTSEFGTLNMWDWEIVAYLEGEGVT
                     ALAPYTLAQTRMLCRAAAARRRLRGSKFLVFQDNPGEGFQAPIFKRFYWWENEATQRL
                     LDKFGITIVKKSFREFGARARAIPNEAAAAEWARWQWPVALPEQPLLSAVKIYLAMRE
                     ELAADSDIVSAGINCLNESHFSDTTPCLAWMMLYEEGRLIWGCEADTLSMATQYILHQ
                     ALDTPIMMTNLYPSLMSGPALKHERIPHFPDVRTYPDPNPENYVLVAHCGYYGLLPPS
                     FGTQWAMRPKVLAIVDENAHAIDARFPIGPVTLAKLHPSMARLTAVEGTLAGYAQFPG
                     SDCLNGGVIHVPDGHRLVRRATSHHYLVLTGHHLNDMSMGLKVFGLEVEPL"
     gene            1959..2795
                     /locus_tag="PK829_10255"
     CDS             1959..2795
                     /locus_tag="PK829_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41636.1"
                     /translation="MTDFYSLPTAGLENEYLTLEYLTHAGPRIVRLIPRRLGQNLFAE
                     TPRQQLQSPYGPFHLWGGHRLWHAPETAARTYIPDDGGLTVEPLPPSAEGEGVCLRYM
                     EPYSGIHKEIRLRLAADAPRVTLTHRLTNLGLWPIELAPWAITQLKIGGTAVLPTPPE
                     SDPEALLPNRMIALWPYSRWDDPRLRLGESVIEVLAQPLATPFKIGYLNLAGWAEYRY
                     EGVTFRKRWTPQPERRHADYGCNVEVYASERHLELETLGPLTRLEVGEMAEHIEVWEV
                     EE"
     gene            2995..3087
                     /locus_tag="PK829_10260"
                     /pseudo
     CDS             2995..3087
                     /locus_tag="PK829_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
     gene            3127..3528
                     /locus_tag="PK829_10265"
     CDS             3127..3528
                     /locus_tag="PK829_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOU41637.1"
                     /translation="MTQDQTEDQPNQLLVYGRPGCPMVPPVRTYLDEAGIAYTYRDIR
                     QDADAAVQLRRLANGFESVPTVVLPNGRVLVEPGVGRLRQALKELHEAGGGSGDPGLP
                     GTLKAGLSNPVYPVLLLIALALAVAIALASG"
     gene            3543..>3859
                     /locus_tag="PK829_10270"
     CDS             3543..>3859
                     /locus_tag="PK829_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HOU41638.1"
                     /translation="MKRALMVVALMVLLAGCTAGSGTTAEPTAASTVPATPRPAASMP
                     AANPAEPTAAVANPASANCTQVGGMMQVEARPDGAEFGVCYFDDNRQCEEWALLRGDC
                     PVGG"
BASE COUNT          631 a         1234 c         1330 g          664 t
ORIGIN      
        1 cgcgggcctg agtctggagg cgattattga gccgggctac ttcggccgca ccggcgcgga
       61 ggtgctgtgc ctgggggcgg gcgggtcagc cgtggccacg ctgctgtacc tgatgaacaa
      121 gcccgacccg gccgaccggc cgcggcgctt catcgtcgtc aaccgctccc aggggcggct
      181 cgaccacttg caggacatgg tggcgcgcat cggcagcgac attgccgtgg aggccatctg
      241 caacgccgac ccgcgcgtca acgacgagat catggcgttc ttgccggagc acagtctggt
      301 catcaacgcc acgggcatgg gcaaggactc gcccggctcg cccatcaccg acgcgggcgt
      361 cttcccgcgc cggggcatcg cctgggagtt caactaccgc ggcgagttgg acttcatgca
      421 ccaggccctg cggcaggtgg acagccgcgc cttgcgcgtg gaagacggct ggatctattt
      481 tgtccacggc tggtcgcagg tggtggccca ggtgctccac gttgacctga cgccagaggt
      541 gtttggccgg ttggagcggg ccgcggcgac ggttcggtga atggccgggc cgaggagcaa
      601 gacatgaaag cgaaactagc gcccatctac ttcgatccgg ggcgagacga gggcttcgac
      661 gggcaactgg cccggctgcg cgagctgttg gccgacgatg ccgagtttct ggagccggtg
      721 gccctggggc gggggctgcc gcgcgaggcc gacgcggccg tcttccccca actgctgggc
      781 gaggcttatc gtcgcgtggg cgatttccgt cgcattgatg tgcccatcct ggtggttacg
      841 tcggagttcg gcacgctgaa catgtgggac tgggagatcg tcgcctatct ggagggggag
      901 ggcgtcacgg cgctggcccc ctacacgctg gcgcagacgc gcatgctctg ccgggcggcg
      961 gctgcgcggc ggcggctgcg cggcagcaag ttcctcgtct tccaggacaa ccccggcgag
     1021 ggcttccagg cgcccatctt caagcgcttc tactggtggg agaacgaggc cacgcaacgg
     1081 ctgctggaca agttcggcat cactattgtc aagaagagct ttcgcgagtt cggggcgcgg
     1141 gcgcgagcca tccccaacga ggccgcggcg gccgagtggg cgcgctggca atggccggtt
     1201 gccctgccgg agcagccgct gctgagcgcg gtcaaaatct acctggccat gcgcgaggag
     1261 ctggccgccg acagcgacat cgtcagcgcg ggcatcaact gcctgaacga gtcgcatttc
     1321 agcgacacga ccccctgcct ggcgtggatg atgctctatg aggaggggcg gctcatctgg
     1381 ggctgcgagg ccgacacgct gtcgatggcc acccagtaca ttctccacca ggcgctcgac
     1441 acgccgatca tgatgaccaa cctgtacccg tcgctgatga gcggcccggc gctgaagcac
     1501 gagcgcatcc cccactttcc cgacgtgcgc acctaccccg acccgaatcc ggagaactac
     1561 gtcctggtgg cccactgcgg ctactacggc ctgctgccgc ccagcttcgg cacgcagtgg
     1621 gccatgcggc caaaggtgct ggccattgtt gacgagaacg cccatgccat cgacgcccgc
     1681 ttcccgattg ggccggtgac gttggccaag ctgcacccgt cgatggcgcg gttgacggct
     1741 gtggagggta cgctggccgg ctacgcccag tttcccggct ccgactgtct caacgggggc
     1801 gtcatccacg tgcccgacgg ccaccggctc gtgcgccggg ccacgtcgca tcactatctg
     1861 gtgctgacgg gacaccattt gaatgacatg agcatgggcc tgaaggtgtt tgggctggag
     1921 gtggagccgt tataatggcc gtcggaggat aaatgcttat gacagacttt tatagcttgc
     1981 cgactgctgg ccttgagaat gagtatctaa ctctggaata cctgacccat gccgggccgc
     2041 gcattgtgcg gctgatcccg cgccggctgg gccagaacct gttcgccgag acgccgcgcc
     2101 aacaactgca atcgccctac gggccatttc acctgtgggg cggccaccgg ctgtggcacg
     2161 cgccggagac ggccgcccgc acctatattc ccgacgatgg cggcctgacg gtggagccgt
     2221 tgcccccctc ggccgagggg gagggcgtgt gcctgcgcta catggagccg tattcgggca
     2281 tccacaagga gattcgcctg cggctggcgg ccgacgcgcc gcgcgtcacg ctgacccacc
     2341 ggctgaccaa cctgggcctg tggccgattg agctggctcc gtgggcgatc acgcagctca
     2401 agatcggcgg cacggccgtc ctgccgaccc cgccagagag cgacccggag gcgctattgc
     2461 ccaaccgcat gatcgccctg tggccctata gccgctggga tgacccccgc ctgcgcctgg
     2521 gcgagtcggt tatcgaggtg ctggcccaac ccctcgccac gccgttcaag atcggctacc
     2581 tgaatctggc cggctgggcg gagtatcgct atgaaggcgt cactttccgc aagcgctgga
     2641 cgccacagcc ggaacggcgg cacgccgatt acggctgcaa cgtggaggtc tacgccagcg
     2701 aacgccacct ggaactggag acgctggggc cgttgacccg gctggaagta ggcgagatgg
     2761 ccgaacacat cgaagtgtgg gaagtggaag agtaggcgga cagggtggcg cgattctccc
     2821 gaatcgcgtt ttgtggagca ggaaaagcga ttgggtggcg cgattctccc gaatcgcgtt
     2881 ccatcgcgtt ctgtggaaat gaagaagagc gattcgtaga atcgcgccac gcgggcattg
     2941 gctgccttga tgttgagagt gtgctatgat gacctcatga caggtggcgc aggtatgcgt
     3001 gatcagcaac ttctcgagcg catcgttctt gatcccaacg tgatggttgg gaaaccagtt
     3061 attcgcggga ctcgtctctc cagcgagcgc cgcgtgcggt ttgcacggat tcaacgttaa
     3121 ggtaccatga ctcaagacca gactgaagat caacccaacc aactgctcgt ctacggccgc
     3181 cccggctgcc ccatggtccc gcccgtgcgg acgtatctgg acgaggcggg cattgcctac
     3241 acctaccgcg acattcgcca ggacgccgac gcggccgtgc agctacgccg gctggccaat
     3301 ggcttcgaga gcgtgcccac ggtggtgctg cccaatgggc gcgtgctggt ggagccgggc
     3361 gtgggccgcc tgcggcaggc actcaaggaa ctgcacgaag ccggaggtgg ttcgggcgac
     3421 cccggcctgc ccggcacgct gaaggccggt ctgtcgaacc cggtctaccc ggttctgctg
     3481 ctcatcgctc tggcgttagc cgtggccatc gctctggcgt cgggctagga ggtgggtacg
     3541 tcatgaagcg cgcgctgatg gtggtggcgc tgatggtctt gctggcgggc tgcaccgcgg
     3601 ggagcggtac cacagccgag ccgacggcgg cctccacagt gccggccacg ccccgcccgg
     3661 ccgcgtcgat gcccgccgcc aatccggctg agccgacggc cgccgtcgcc aaccctgcct
     3721 cggccaactg cacccaggtg ggtggcatga tgcaggtcga ggcccggccc gacggggccg
     3781 agttcggcgt gtgctatttt gatgacaacc ggcagtgtga ggagtgggcg ctgttgcgtg
     3841 gcgattgccc cgtgggtgg
//
LOCUS       DAOSMQ010000304         3835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23866_length_3835_cov_4.216931, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000304 DAOSMQ010000000
VERSION     DAOSMQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23866_length_3835_cov_4.216931"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK829_10275"
     CDS             complement(<1..637)
                     /locus_tag="PK829_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41639.1"
                     /translation="MSESPVDDPSTADGKLPSTDTPPEAINLNVGGRELAGPSRGFGQ
                     LWRKRYRVRLVGAEVTPEEVIRRWRARFADYWPEGSDFYGSRPRIEAGEVAVINLEGP
                     AGAPLATGVAVIRADDRSFAFMTPQGHIFAGTITFSAYTAADDRLFPGVTVAQVESLI
                     RAGDPLFEIGARLGIIHRREDDFWEQTLTRLAADFNVRGQPVEIESSLLDDH"
     gene            complement(700..1077)
                     /locus_tag="PK829_10280"
     CDS             complement(700..1077)
                     /locus_tag="PK829_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU41640.1"
                     /translation="MSSNYQQPNVQTAVRQEGDAGIIAIQGEVTGFAEDALMSAYAQA
                     SQGATPVIILDFTGLEYMNSTGIGLLVTLLIRAQRQQQRLLACGLSDHYRQIFDLTRL
                     NEAIGIYDSQAEALAAVAAAEGR"
     gene            complement(1100..1462)
                     /locus_tag="PK829_10285"
     CDS             complement(1100..1462)
                     /locus_tag="PK829_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU41641.1"
                     /translation="MEPQSDSFRVQVRRRDGLAVLDLFGDIDGFADVALDAAYSEAEA
                     FATPTIMLHFGNVGYINSTGIALIVGLLARARKTQRRLVTCGLSEHYREIFNITRLSD
                     FMTVYADEESALREVAVA"
     gene            complement(1446..>2653)
                     /locus_tag="PK829_10290"
     CDS             complement(1446..>2653)
                     /locus_tag="PK829_10290"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU41642.1"
                     /translation="ERQRIEQELRVARLIQQFLLPKSVPDIAGWEVTAYYQPARAVGG
                     DFYDFISFPDGRIAFVIGDVTDKGVPAALVMATTRTLLRSAAERLVSPGAVLEETNNQ
                     LVENIPPKMFVTCLYALLDPASGRLVFANAGHDVPYRRTRAGVVELRARGMPLGLLPN
                     MHYEQHEVTLETGDCVLLYSDGLVEAHSPTREMFGFPRLQAMMGEPVDGNLVPFLLDR
                     LRAFTGPQWEQEDDVTIVLLGSRPGGNGAGEPEERRKVLARFELPSRPGNERLAMQQV
                     AEVVAPLGLAQGKLDRLKTAVAEATMNGMEHGNRYDEDIPVLIEVARETDDLTISITD
                     RGLKQPIPEAATPDLTAKLAGQQSPRGWGLFLIQNMVDEMHVTADEVHHTVNLVVHLT
                     EGAQRGTTE"
     assembly_gap    2654..2753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2754..>3835)
                     /locus_tag="PK829_10295"
     CDS             complement(<2754..>3835)
                     /locus_tag="PK829_10295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU41643.1"
                     /translation="GGGPAGVTAALRARELGATVALVERGRLGGTCTNDGCVPTRVLA
                     HAARLLRETAQHEAYGLIGPRPAVDFARLIDSTHRVVAAVHEKKYLIKHLEDSGVETY
                     LGAGPARFVDAHTIETAGGDRFSAERFILCLGGHARKLPFPGAELALTHGDVWNLREL
                     PRRLAVVGGAATGCQLASIMNTFGSKVTLLDVAPQILPIEDTAVAEALTTAFTDRGIT
                     IISGMSGVERLEKSDDGLRLVYKKDDAPHELAVDAVIVAVGWPGNVEGLGLETVGVKT
                     ERNYITVDDALRTSAPHIFAAGDVNGRMMLVQSAGYEARIAAENAVASRGDGDMTEYC
                     PQIVPHGGFTDPEYGSVGLTEKAARAA"
BASE COUNT          571 a         1223 c         1299 g          642 t
ORIGIN      
        1 tgtggtcgtc gagcaggctg ctttcgatct cgaccggctg gccgcgcacg ttgaagtcgg
       61 ccgccagccg cgtcagcgtc tgctcccaga agtcgtcctc gcgccggtgg atgatgccca
      121 gccgcgcgcc gatctcgaat agtgggtcgc cggcgcggat gagcgactcg acctgggcta
      181 cggtgacacc gggaaacagg cgatcgtcgg cggcggtgta ggcactgaag gtgatggtgc
      241 cggcgaagat gtgtccctgg ggggtcatga aggcgaagga gcggtcatcg gcgcggatga
      301 cggcgacgcc cgtggccagg ggcgcgccgg ccggcccttc gaggttgatg acggccactt
      361 cgcccgcctc gatgcgcggc cgcgaaccgt agaagtcact gccctctggc caatagtcag
      421 cgaagcgcgc ccgccaacgg cggatgactt cttccggcgt cacttccgcg cccaccaggc
      481 gcacgcggta gcgcttgcgc cacagttggc caaagccacg gctcggcccg gccagctcgc
      541 gtccgccaac attcaggttg atcgcttcgg gaggggtatc agtgctgggt agtttgccgt
      601 cggcggtcga tggatcgtcg acaggggact cactcatggc ccctatcgta gccagaactt
      661 gcgggccggc aaattgccgc tggtttactc acccatgcct tagcgcccct cggccgcggc
      721 tacggccgcc agcgcctcgg cctgcgagtc atagatgccg atggcctcgt tcagccgcgt
      781 caggtcaaag atctggcggt agtggtcgct cagaccgcag gccagcagcc gttgctgctg
      841 gcgctgggcg cggatgagca gcgtcaccag caggccgatg ccggtgctgt tcatgtactc
      901 cagaccggtg aagtccagga tgataaccgg ggtcgcgccc tggctggcct gggcataggc
      961 cgacatcagc gcgtcctcgg cgaagccggt gacctcgccc tggatggcga tgatgcccgc
     1021 atcgccttcc tggcgcaccg cggtctgtac attaggctgc tggtaatttg agctcatgct
     1081 cgtgcttcct cggccggcgc taagcgacag ccacttcgcg tagggcgctc tcctcgtcgg
     1141 cgtagacggt catgaagtcg gagaggcggg tgatgttgaa gatctcgcgg tagtgctcgc
     1201 tcaggccgca ggtgaccagc cgccgctgcg tcttgcgggc gcgggccagc agcccgacga
     1261 tcagggcgat gcccgtcgaa ttgatgtagc cgacgttgcc gaagtggagc atgatggtcg
     1321 gtgtggcaaa cgcttcggcc tcgctgtaag cagcgtccag agcaacatcg gcgaagccgt
     1381 cgatgtcgcc aaaaaggtcg agcacggcca atccatcgcg gcggcgcacc tgcacacgaa
     1441 acgaatcact ctgtggttcc acgttgcgct ccttcggtca ggtgaacgac caggttaacc
     1501 gtgtgatgca cttcgtcggc cgtcacgtgc atctcatcga ccatgttctg aatgaggaac
     1561 agcccccagc cgcggggcga ttgctggccg gccagcttgg cagtcaggtc gggggtcgct
     1621 gcttcgggga tgggttgctt caggccgcgg tcggtgatgc tgatggtcag atcgtcggtc
     1681 tcgcgggcta cttcgatgag caccgggatg tcctcgtcgt accggttgcc gtgctccatg
     1741 ccgttcatgg ttgcctcggc cacggccgtc ttcagccggt cgagcttgcc ctgcgccagg
     1801 cccagggggg cgacgacctc agccacctgt tgcatcgcca ggcgctcgtt gccgggccgg
     1861 ctgggcagct cgaagcgggc cagtaccttg cgcctctcct ccggctcgcc ggcgccgttg
     1921 ccgccgggcc gcgaaccgag cagcacgata gtcacgtcgt cttcctgctc ccactgcggg
     1981 ccggtgaagg cgcgcaggcg gtcgagcagg aagggcacca ggttgccgtc gaccggctcg
     2041 cccatcatcg cttgcagtcg cgggaagccg aacatctcgc gcgtggggct gtgggcctcc
     2101 accaggccat cgctgtagag cagcacgcag tcacctgtct ccagcgtgac ctcgtgctgt
     2161 tcatagtgca tattgggcag cagccccagg ggcatgcccc gtgcgcgcaa ctcgaccacg
     2221 cccgcgcgcg tgcgccggta gggcacgtcg tggccggcgt tggcaaagac gaggcggccg
     2281 ctggccgggt cgagcagagc gtagaggcag gtaacgaaca tcttcggcgg aatgttttcg
     2341 accaactggt tgttggtttc ctccagcacc gcgccgggcg acaccagccg ctcggccgcc
     2401 gagcgcaaca gtgtgcgcgt cgtagccatt accagcgcgg cgggcacgcc cttgtcggtc
     2461 acgtcgccga tgacgaaggc gatgcggccg tcggggaagg aaatgaagtc atagaaatcg
     2521 ccgcccacgg cccgcgcggg ctggtagtag gcggttactt cccagccagc gatgtcgggc
     2581 acgcttttgg gcagcaggaa ttgctggatg agccgggcaa cgcgcaattc ctgctcgata
     2641 cgctggcgct ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggcgc
     2761 gggcggcctt ctccgtcagg ccgacgctgc catattcggg gtcggtgaag ccgccatggg
     2821 ggacgatctg cgggcagtat tcggtcatgt ccccgtcgcc gcggctggcc acggcgttct
     2881 cggcggcgat gcgggcctcg tagccggcgc tctggacgag catcatgcga ccgttcacgt
     2941 cgccggcggc gaagatgtgg ggcgcgctgg tgcgcagggc gtcatcgacg gtaatgtagt
     3001 tgcgctccgt tttcaccccc accgtctcca gccccagccc ctcgacgttg cccggccagc
     3061 cgacagcgac gatgacggca tcgacagcca gttcgtgcgg cgcgtcgtcc ttcttataga
     3121 ccaggcgcag cccgtcgtcg cttttctcca gccgctcgac gccgctcatg ccgctgatga
     3181 tagtgatgcc gcggtcggtg aacgccgtgg tcaacgcctc ggccacggcg gtgtcctcaa
     3241 tgggcaggat ttgcggcgcg acgtcgagca gcgtgacctt cgagccgaag gtattcatga
     3301 tcgacgccaa ctggcagccg gtggccgcgc cgccgacgac ggccagccgc cgcggcaact
     3361 cgcgcaggtt ccacacgtcg ccgtgggtca gagccagttc cgcgccgggg aagggcagct
     3421 tgcgagcgtg gccgcccagg cagaggatga agcgctcggc gctgaagcgg tcgccgccgg
     3481 ccgtctcgat ggtgtgggca tcgacgaagc gggccgggcc ggcgccaaga tacgtttcga
     3541 cgcccgaatc ttccaggtgc ttgatcaggt acttcttctc gtggacggcg gccacgaccc
     3601 ggtgggtgct gtcgatgagg cgggcgaagt ccaccgccgg ccgcgggccg atcaggccat
     3661 aggcttcatg ctgcgccgtc tcgcgcagca gccgggcggc gtgggccagc acgcgcgtgg
     3721 gcacgcagcc gtcgttggtg catgtgccgc ccagacggcc gcgctccacc agggcgacgg
     3781 tcgcgcccaa ctcgcgggcg cgcagggcgg cggtgacacc ggccgggccg ccacc
//
LOCUS       DAOSMQ010000305         3832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23891_length_3832_cov_4.649192, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000305 DAOSMQ010000000
VERSION     DAOSMQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23891_length_3832_cov_4.649192"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..>1105)
                     /locus_tag="PK829_10300"
     CDS             complement(128..>1105)
                     /locus_tag="PK829_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOU41644.1"
                     /translation="LFRRMDWDVVLSRLKHDGKLQLTTGDTTLALDDLVNVVGIPEEV
                     EKAASWLGEPAGEQLELDRSQYDFRRIFVSNPAIVGRSLNELDLPRSYGAIVSRVRRG
                     DIDLLAHGDTVIELGDRVRVVAPRTDMPRLTELFGDSYKSLSEVNLLTLGIGLAIGVL
                     IGLIPIPLPGGVVISLGMAGGPLLVGLVLGALRRTGGLIWTIPYSANLTLRQIGLTLL
                     LAGIGIRSGYTFLTNFADSGGTQVFFAGLVITTVIAFLTLWLGYRVLRIPFGMLTGML
                     AGLGTQPATLGFSLQQADNELPNLGYALVFPVATITKVIVAQLLLLLLR"
     assembly_gap    1106..1205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1206..1508)
                     /locus_tag="PK829_10305"
     CDS             complement(<1206..1508)
                     /locus_tag="PK829_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOU41645.1"
                     /translation="MQAVVDFLVEEPLLLLFIVAGLGYLLGNVKIKGISLGVAAVLFV
                     GLAFGALSPDMKLPSVIVDLGLIIFVYTIGLSSGPGFFASFKRKGLRDNTFVFLLLL"
     gene            1798..2223
                     /locus_tag="PK829_10310"
     CDS             1798..2223
                     /locus_tag="PK829_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU41646.1"
                     /translation="MKTLRVMLLALALLGGAALVACRGGETAGPAIEEIDLTTLDDEV
                     DVETVEAIRQNPAVFLLDVREPEEYAAGHIPNITLIPSGEVAARLAELPRDKTIIVTC
                     RTGNRSAQVADLLRDQGFTNVHNMIGGIVAWEEAGYPVE"
     gene            2294..3616
                     /locus_tag="PK829_10315"
     CDS             2294..3616
                     /locus_tag="PK829_10315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41647.1"
                     /translation="MTRLGRGLAAYAAMIRLFSRNVWLVLLFGIGTGLTFGIFLFTFN
                     FYVLSLGPGYDEAFLGTLTSAASFATIAMALPAAYLAQRHSAKGVMIAAGLVGMAAYL
                     GLVLFPARLPLVAFRMLAGVAMSLGTVAVAPFLMANTGARERQWVFSFQAGLSTVASF
                     FGNLLAGMLPGRLGALAGVGPTETLAYRLALGSMVALSGLSILPLLFIRRAARSDAAV
                     ELPWVQLRRYGRLFARFLLPSLVIGLGAGLMQPFMNVYFRNVYQKPDPAIGVVFALGG
                     LAMAIGQFAGPPLADRLGKIRTVMLTQALSIPFLMTLGLGAWLVPTGRAAAGTIFVVA
                     GVAYIFRLALMNLSNPVYQTFILERVPAQAQALSVSLSNLVFQVGWFIMPQVSGQLQV
                     AHGPVGFTAVFAGVTVFYAVAIGLEWLFFSAEARQEAQAERLLVGKRA"
BASE COUNT          612 a         1183 c         1321 g          616 t
ORIGIN      
        1 cggccacgct gccgtagatg acgcggtaga aggccacggc ggcgaccacg cccagcaggg
       61 ccacggccag ggccggcagc cagaggttcg cccgtcggcc gggggcggag acatgctcgt
      121 ccatgcccta gcgcagcagc agcaacaaca actgggcgac gatgaccttg gtgatcgtcg
      181 ccacggggaa gacaagggcg tagcccagat tgggcaactc gttgtcggcc tgttgcaggc
      241 tgaagccgag ggtggccggc tgcgtgccca ggccggccag catacccgtc aacatgccga
      301 aggggatgcg cagcacgcgg tagcccagcc acagcgtcag gaaggcgatg acggtggtaa
      361 tgaccagccc ggcgaaaaag acctgcgtgc cgccgctgtc ggcgaagttg gtcaggaaag
      421 tgtagccaga gcggatgccg atgccggcca gcagcagcgt cagaccgatc tggcgcaggg
      481 tgagattggc gctgtagggg atggtccaga tcaggccgcc tgtccgccgc agcgcgccca
      541 gcaccagccc aacgagcagc ggcccgccgg ccatgcccag gctgataacc acgccaccgg
      601 gcagggggat ggggatgagg ccgatgagca cgccgatggc caggccaatg cccagggtca
      661 gcaagttgac ctcgctgagc gacttgtagg aatcgccgaa tagctcggtc aggcggggca
      721 tgtccgtgcg cggggcgacg acgcgcacgc ggtcgcccag ctcgatgacg gtgtcgccgt
      781 gggccagcag gtcgatgtcg ccgcggcgga cgcgggagac gatggccccg tagctgcgcg
      841 gcaggtcgag ttcgttaagg gagcggccga cgatcgcggg gttgctcaca aagatgcgcc
      901 ggaagtcata ctggctgcgg tccagttcca gctgttcgcc cgccggctcg cccagccaac
      961 tggcagcctt ctcgacttct tcgggaatgc cgacgacgtt gaccagatcg tccagggcca
     1021 gggtggtgtc gccggtggtg agctggagct tgccgtcgtg cttcaggcgc gagaggacga
     1081 catcccaatc catgcgccgg aacagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnngagca gcagcaggaa gacgaaggtg ttatcgcgca ggcccttgcg cttgaaagag
     1261 gcgaagaagc ccggcccgct gctcaggccg atggtgtaaa cgaagatgat caggccgaga
     1321 tcgacgatga ccgacggcag cttcatgtcc gggctgaggg cgccgaaggc caggccaacg
     1381 aacagcaccg cggccacgcc caggctgatg cccttgatct tgacgttgcc gagcagatag
     1441 cccaggccgg caacgataaa taataggagg agtggctcct caacgaggaa atcgacgact
     1501 gcttgcatga cgcgcgctcc aggccggcgt gacggccgcg ccggggccgg ctctgaatga
     1561 gtatagcatg gccatccgca ggcgcacgtg gagcggggag ggacacggac atgacaggag
     1621 aagctcacgc aaacgcgcgc gccagggtca ggtaggcggg ctaaaagagc atttccgcga
     1681 caggactttg gaaaagaccc gcgccccagc gcgtgggctt gtattaccgg ccctacttga
     1741 gtacgatccg tgttagccct cttcgcggag cggcacaaca aggactctgg aggcagtatg
     1801 aaaacactgc gtgtgatgct tctggcgctg gcgctgctgg gcggcgcggc gctggtggcc
     1861 tgtcgtggcg gcgagacggc cggcccggcc attgaggaaa tcgatctgac aacactggac
     1921 gatgaggtcg atgtggagac ggttgaggcc attcgccaga acccggccgt cttccttctg
     1981 gacgtacgcg agccggagga atatgccgcc ggccacatcc ccaacattac cctcatccca
     2041 tcgggtgagg ttgccgcccg gttggcggag ttgccgcgcg acaagacgat catcgtcacc
     2101 tgccgcaccg gcaaccgcag tgcccaggtg gccgatctgc tgcgcgacca gggcttcacc
     2161 aacgtccaca acatgatcgg cggcatcgtc gcctgggaag aggccggcta ccccgtcgaa
     2221 taacagccgt tcagcgcgcc ccgtcgcgcc gccggccgcg gccgttcctg gcccggccgg
     2281 caaggatatc cccatgaccc gccttgggcg cgggctggcc gcctacgcgg ccatgatccg
     2341 cctgttctcg cgcaacgtct ggttggtgct gctgttcggc atcggcacgg gcctgacgtt
     2401 tggcatcttc ctgttcacct tcaacttcta cgttctcagc ctggggccgg gctacgacga
     2461 ggccttcctg ggcacgctga cgtcggcggc ctcgtttgcc accatcgcca tggccttgcc
     2521 ggcggcctat ctggcccagc gccactcggc caaaggggtg atgatcgccg ccgggctggt
     2581 cggcatggcc gcctacctgg ggttggtgct gtttccggcg cggctgccgc tggtggcctt
     2641 tcggatgctg gccggcgtgg ccatgagcct gggcacggtg gctgtcgcgc cgtttctgat
     2701 ggccaacaca ggggcacgcg agcggcagtg ggtcttcagc ttccaggccg ggctatcgac
     2761 cgtggccagc tttttcggca acctgctggc tggaatgcta cccggccggt tgggggcgct
     2821 ggcgggcgtg gggccaacgg agacgctggc ctaccgattg gccctcggca gcatggtcgc
     2881 tctcagcggg ctgtcgatcc tgccgctgct gttcatccgc cgcgccgcgc gcagcgacgc
     2941 ggcggtggag ttgccgtggg tgcaattgcg gcgctatggg cggctgttcg cgcgcttttt
     3001 gctgccgtcg ctggtcatcg gcctgggcgc ggggctgatg cagccgttca tgaacgttta
     3061 ctttcgcaac gtctaccaga agcccgaccc agccatcggc gtcgtcttcg ccctgggcgg
     3121 gctggccatg gccattggcc agttcgccgg gccgccgctg gccgaccggc tgggcaagat
     3181 tcgcacggtg atgctgacac aggcactgtc gatcccgttc ctgatgacgc tggggctggg
     3241 ggcgtggctg gtgcccacgg ggcgcgccgc cgcggggacg atcttcgtcg tcgccggcgt
     3301 ggcctacatc ttccggttgg cgctgatgaa cctgagcaac ccggtctacc agacgttcat
     3361 cctggagcgc gtgccggcgc aggcccaggc gctgtcggtc agtctgtcga acctggtctt
     3421 ccaggtgggc tggttcatca tgccgcaggt cagcgggcag ttgcaggtgg cgcatgggcc
     3481 ggtggggttc acggccgtct tcgccggggt gacggtgttc tatgccgtgg ccatcggtct
     3541 ggagtggctg ttcttcagcg ccgaggcgcg gcaggaagcc caggccgagc ggctgctggt
     3601 ggggaagcgg gcctagcggg ccagcggcgg agccagttgg cgacaaaaaa cggcggccgc
     3661 cgttgccggg gccgccgttc gggttcttgg cttacgtcgc ctgacagtga aggtctaaca
     3721 gattcttacc aacggttgcg gtcgccgcgg tcgcgatcgc cgcgaccacc ctgaccaccg
     3781 ccatagccgc cacgcccgcc gctgctaccg cggctatcgg gccggccgcc ga
//
LOCUS       DAOSMQ010000306         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_23978_length_3822_cov_4.606318, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000306 DAOSMQ010000000
VERSION     DAOSMQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23978_length_3822_cov_4.606318"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PK829_10320"
     CDS             complement(<1..642)
                     /locus_tag="PK829_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41648.1"
                     /translation="MAVTTDTGLSPRPSTAPMTRGRDAFHQVAAILLLTQAILWVFLL
                     FRWATFSRFNWNAYAAELTLAQWPPLLVYGLYGLLAVWAAAAAVGLRRGARWGRAAGL
                     AYALTTMAGALLYYLLGREFVGAIVVIAAAGLLLCLLTRRAAWSLAYPAAFWLLVFFL
                     IPLAIVFFVSLGERTRLGTVTYPPLSLGSLGALFDDYARVFSRVGGDFIYLRIF"
     gene            complement(832..2037)
                     /locus_tag="PK829_10325"
     CDS             complement(832..2037)
                     /locus_tag="PK829_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41649.1"
                     /translation="MTDSFRVELRDVCKQFGDTLAVDSLSLQIRDGEFFSLIGPSGCG
                     KTTTLRMIAGFEQPTDGAIFISGQHVEGIPAHRRPVNTVFQNYALFPHMNVAQNVAFG
                     LEMSKVPAPEIKRRVAEALELVRLPQMAERKPKQLSGGQQQRVALARALVNRPQVLLL
                     DEPLGALDLKLRKAMQSELKQIQTEVGITFIYVTHDQEEAMAMSDRIGIMNEGVLQQV
                     GSPHEVYEKPNNRFVADFIGETNFLPATVVRLETEDEHPVVMLQGNVPVLAADEAHDL
                     RLGQAVTLTLRPERINLYPQGKVDVLKAESGLEAEELERILGAKMPGAVDMKEFLLAE
                     PENVVLDGVLEEAFYIGTDTRFRVTLEGGASLVVRQQNYGSRYDLPFDVGSRVYVQWA
                     AENAQILID"
     gene            complement(2296..>3822)
                     /locus_tag="PK829_10330"
     CDS             complement(2296..>3822)
                     /locus_tag="PK829_10330"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU41650.1"
                     /translation="RWTVRTDRLAALKVPATLTGLLQARLDGLPRPEREALQRAAVVG
                     RLFWDDCVADLLETERAVIEPTLDAIQGRELIFHREHSTFTQAGEYIFKHALLRDVAY
                     ETVLLKYRAEFHGKVARWLEENAGERIGEYLGVIAEHFAQAGEFDRAADYQQQSGEQA
                     LRAAAFRPARAAFERALALRQRGTAAAGAALPHCLGLGEVCYRLSDFPAASDALSRAL
                     ALARAGGDTRAQAEALSWTALVAISVGRAAEAGPLLEEALALARPIGGATLAQVLIHS
                     GNYHWQQGDLDTAGAYAEEVLRLSQALGDVLLQGNALRILGVIAGLRGDFDTSIRHFE
                     AGMALARQIGDRAQESKSLANLGATRHYAGDYAGAVADYLAAFEVDQELGLTESMAFT
                     AHNLADVYNALGQLAESRRFAVECIRLARPVGALPLQLAALVPLAQVLLKEGETGRAL
                     ALLGLARNHPSTPNQKQIGIQEALATVTLDPAEVEAALVAGAALDFDTVVGEILDGEW
                     "
BASE COUNT          643 a         1307 c         1269 g          603 t
ORIGIN      
        1 gaagatgcgc agataaatga agtcgccacc gacgcggctg aagacgcggg catagtcgtc
       61 aaagagcgcc cccagcgagc cgagactcag cggcgggtag gtaaccgtgc ccagccgcgt
      121 gcgctcgccc aggctgacga aaaagacgat ggccaggggg atgagaaaga agaccagcag
      181 ccagaaggcg gctgggtagg ccagcgacca ggccgcgcgg cgcgtcaaca ggcagagcag
      241 tagccccgcg gcggcgatga cgacgatcgc gccgacgaac tcccgaccga gcaggtagta
      301 gagcagcgcc ccggccatcg tcgtgagcgc ataggccagg ccggccgccc gcccccagcg
      361 tgccccgcgc cgcagcccga cggcggccgc ggcggcccag acggccagca gcccatacag
      421 cccgtagacg agcagcggcg gccactgcgc cagagtcagc tcggcggcat aggcattcca
      481 gttgaaacgg ctaaaggtgg cccagcgaaa gaggaggaag acccagagga tagcctgggt
      541 gaggagcaag atggccgcga cctggtggaa ggcgtcgcgg ccgcgggtca tcggcgcggt
      601 ggaaggtcgc gggcttagcc cggtgtcagt cgtgacagcc atcggctcct cctgctgttg
      661 tggaatcggc tgccgccggg gtggcgttgc tttctgtcca tacgtctcct ataacaggga
      721 ggggagctca cgcaaaggac gctaaggaag caaagagcgc caagaagaac gagataactt
      781 tctcttgttt gcgtccttag ccgccttggc gtcctttgcg tgagcctcct tctagtcgat
      841 cagaatctgc gcgttttcgg cggcccactg cacatagacg cggctgccca cgtcgaaggg
      901 caaatcgtag cgcgagccgt agttctgttg ccggacgacc agcgacgcgc cgccctccag
      961 cgtcacgcgg aagcgcgtgt ccgtgccaat gtagaacgcc tcttccagca cgccgtcgag
     1021 gacgacgttc tccggctcgg ccagcaggaa ctccttcatg tccaccgcgc cgggcatctt
     1081 ggcccccagg atgcgctcca actcctccgc ctccaggccg ctctcggcct tgagcacatc
     1141 gaccttgccc tgcgggtaga ggttgatgcg ctccggccgc agggtcagcg tgacggcctg
     1201 gccgaggcgc aggtcgtggg cctcgtccgc ggccagcacg ggcacgttgc cctggagcat
     1261 caccacggga tgctcatcct ccgtctccag acggacgacg gtggcgggca ggaagttcgt
     1321 ctcgccgatg aagtcggcca cgaaacggtt gttgggcttc tcatagactt catgcggcga
     1381 gccgacctgc tggagcacgc cctcgttcat gatgccgatg cggtcggaca tggccatggc
     1441 ctcttcctgg tcgtgggtga cgtagatgaa ggtgatgccg acttcggtct gaatctgttt
     1501 tagctccgac tgcatcgctt tgcgcagctt caggtctagc gcgcccagcg gctcgtcgag
     1561 cagcagcact tgcggccggt tgaccagcgc ccgggccagg gcgacgcgct gctgctggcc
     1621 gccggagagc tgctttggct tgcgctcggc catctgcggc agccgcacca gttccagcgc
     1681 ctcggccacg cgccgcttga tctccggcgc gggcactttg ctcatctcca ggccaaaggc
     1741 gacgttctgg gcgacgttca tgtgggggaa gagggcgtag ttctggaaga cggtattgac
     1801 cggccggcgg tgggcgggga tgccctcgac gtgctggccg ctgataaaga tggccccgtc
     1861 cgtcggctgc tcgaagccgg cgatcatgcg caacgtcgtc gtcttgccgc aaccgctggg
     1921 gccgatgagc gagaagaact cgccgtcgcg gatttgcagg ctgagcgaat cgacggcgag
     1981 cgtgtcgcca aattgcttac agacgtcgcg gagttcgacg cggaacgaat ctgtcatagc
     2041 catctccctg cgcggtccca atcgccgcta tgacacggcc ggcttgtctc ctcatcggag
     2101 cgcttgcgcg aaaaaaaagc ccggcgccgc ccacccgttg cggtgtccgg cccagaactc
     2161 agcgcaattc gtattcgccg ccacccaccc ggcctctgca tggaactgat tcgtccacag
     2221 gtggcttgcg tgcttgtaat gacgccgtta cgataccctg attgacgcgg attagcaata
     2281 gagaccggtt cgcccctacc attctccgtc gagtatctcc cccaccaccg tgtcgaaatc
     2341 cagcgccgcg ccagcgacca gcgccgcctc cacctcggcc gggtcgagcg tcaccgtggc
     2401 cagcgcttcc tggatgccga tctgcttctg gttgggcgtg ctggggtggt tgcgggccag
     2461 gcccagcagc gccagcgccc ggccggtctc gccctctttg agcagcacct gagccagggg
     2521 caccagcgcc gccagttgca gcggcagcgc gcccaccggc cgcgccaggc ggatgcactc
     2581 cacggcaaag cgccggctct cggccaactg gcccaacgcg ttgtagacgt cggccaggtt
     2641 atgggcggtg aaggccatcg actcggtcag ccccagttcc tgatcgactt cgaacgcagc
     2701 cagatagtcg gccacggctc cggcatagtc gccggcatag tgcctggttg cccccagatt
     2761 ggccagactc ttagactcct gcgcccggtc gccgatctgg cgggccaggg ccatgccggc
     2821 ctcgaaatga cggatcgagg tgtcgaagtc gccccgcagg ccggcgatga cgcccaatat
     2881 ccgcagcgca ttaccctgta gcaacacgtc gcccagggcc tggctcagac ggaggacttc
     2941 ctcggcatac gccccggccg tatcaaggtc gccctgctgc caatgatagt tgcccgagtg
     3001 gatgagaacc tgcgccagtg tcgcgccgcc gatcggacga gcgagggcca gcgcctcttc
     3061 caggagaggg ccggcctcgg cggcccggcc cacactgatg gcgaccagcg ccgtccagct
     3121 gagcgcctcg gcctgggcgc gggtgtcgcc gccagcgcgg gcgagcgcca gcgcccgcga
     3181 gagcgcgtcg ctcgcggccg ggaagtcgct cagccggtaa catacctcgc ccaaccccag
     3241 gcagtggggc agcgccgcgc ccgccgcggc cgtgccccgc tggcgcaagg ccagggcgcg
     3301 ctcgaaggcc gcccgcgcgg gccggaaggc ggccgcccgc agcgcctgct cgcccgattg
     3361 ctgctggtaa tcggcggcgc ggtcgaactc gcccgcctgg gcgaagtgtt cggcaatgac
     3421 gcccaggtac tcgccgatgc gctcgccggc gttttcctcc agccagcggg cgaccttgcc
     3481 gtggaactcg gcccggtact tcagcagcac cgtctcatag gccacgtcgc gcagcagggc
     3541 gtgcttgaag atgtactcgc cggcctgggt aaaggtcgag tgctcgcggt gaaagatcaa
     3601 ctcccgaccc tgaatggcgt ccagcgtcgg ctcaatgacc gcgcgctctg tctccagcag
     3661 gtcggccacg cagtcgtccc agaacagccg gccgacgaca gccgcccgct gcagcgcctc
     3721 ccgctccggc cgcggcaggc cgtccaggcg ggcctgtaag aggccggtca gcgtcgccgg
     3781 gaccttcagg gcggccagcc tgtctgtccg caccgtccag cg
//
LOCUS       DAOSMQ010000307         3810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24080_length_3810_cov_3.370173, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000307 DAOSMQ010000000
VERSION     DAOSMQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24080_length_3810_cov_3.370173"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PK829_10335"
     CDS             <1..142
                     /locus_tag="PK829_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOU41651.1"
                     /translation="YSGHGASTDIVVASARAYLSALNKLLAERQRQAAKPQASNGHAA
                     VA"
     gene            147..953
                     /locus_tag="PK829_10340"
     CDS             147..953
                     /locus_tag="PK829_10340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41652.1"
                     /translation="MTTATDSRSDAVWQAEALSNTYLDGVRGAIPLAAEQIDILVRLA
                     RAARPSARAVLDLGCGDGILGHALLDAYAQTEPTVVLADFSPPMLEAARARLGAHANG
                     HGGRVHFAQLDYAQAAWPGHVIGGPLPRRYDVIVSGFSIHHQPDSRKRRIYADIYALL
                     APGGIFLNLEHVASASRWGEVQFEEYFIDALHRFHGPRGKTREQVAADFYHRPDKTAN
                     LLSPVETQLKWLREIGFSHVDCFFKIFELAIFGGIRPAVAAFTFCGPPFA"
     gene            994..2403
                     /gene="leuC"
                     /locus_tag="PK829_10345"
     CDS             994..2403
                     /gene="leuC"
                     /locus_tag="PK829_10345"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOU41653.1"
                     /translation="MTANPQTIIDKIWQSHIVTEQPDTPAVLYVDLHLIHEVTSPQAF
                     SGLRARGLPVRRPEQTVATMDHSIPTTPLHLPIIDVMAAAQIAQLEQNCRDFGIPLLG
                     FHSPHRGIVHVIGPELGLTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLASQC
                     LLQNKPKTYAVNVEGALRPGVTAKDIILALLAKIGVGGGTGHVFEYRGAAIRALSMEE
                     RMTVCNMSIEGGARAGLIAPDETTFNYLAGRPFAPQGAAWDAAVAGWRALPSDAGARF
                     DKSVDIDADALEPMITYGTNPGMGLPITAPVPDPADAADLTQRLTLQRALDYMDLQPG
                     RPLLGHPIDVVFIGSCTNSRISDLRQAAQLMAGRRVKDGVRVMVVPGSQAVKRQAEAE
                     GLDRIFRAAGAEWREAGCSMCIAMNGDQLAPGQYAVSTSNRNFEGRQGKGGRTFLASP
                     LTAAATAVAGAIADARELV"
     gene            2459..3073
                     /gene="leuD"
                     /locus_tag="PK829_10350"
     CDS             2459..3073
                     /gene="leuD"
                     /locus_tag="PK829_10350"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOU41654.1"
                     /translation="MEPFTTLTGRLIAIPKAHIDTDQIIPARFLKTISKAGLGQNLFA
                     DWRYDGEGQPVADFPLNRPEHAGARILLAGDNFGCGSSREHAPWALADYGFQAIISTS
                     FADIFRNNALKNGLLPVIVDDDTHEQLFSLVGEDPTTEITIDLDTQSLLLPDGRRVTF
                     PIDAFSKVCLLEGLDQLGYLLKHGPAVAAFEATHPQRVNTGVRD"
     gene            3182..>3810
                     /locus_tag="PK829_10355"
     CDS             3182..>3810
                     /locus_tag="PK829_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOU41655.1"
                     /translation="MQPIHLYDTTLRDGTQREGISYSLDDKLKIAARLDAFGMDYIEG
                     GWPGSNPKDVEFFRRAPSLGLRHARLAAFGSTRRKDTRPADDANIAALLGAETPVVTL
                     VGKSWGLHVVDVLETTPAENLAMIGDSVAYCATQGKEVIYDAEHFFDGYRADPAYALA
                     TLEAAARGGAACVVLCDTNGGALPWDVEAIAGRVIAQLGPGVPVGIHTH"
BASE COUNT          663 a         1390 c         1177 g          580 t
ORIGIN      
        1 ctacagcggc cacggcgcga gcaccgacat cgtcgtggcc agcgcccgcg cctatctgag
       61 cgcgctcaat aagctgttgg ccgagcgcca gcgccaggcg gccaagccgc aagccagcaa
      121 cggccatgcc gccgtcgcct agcgccatga ccacggccac cgatagccgc agcgatgccg
      181 tctggcaagc ggaagcgctc agcaacactt acctcgacgg tgtgcgcggc gcgatcccac
      241 tggcggccga gcagatcgat atcctcgtgc ggctggcgcg agcggcgcgg ccgtcggcgc
      301 gggctgtcct cgacctcggc tgtggcgacg gcatcctcgg ccacgccctg ctagacgcct
      361 acgcccagac cgagccgacc gttgtcctgg ccgacttctc gccgccgatg ctggaggcgg
      421 cgcgcgcgcg gctgggcgcg cacgccaacg gccacggcgg ccgggttcac ttcgcccagc
      481 ttgactacgc gcaagcggcc tggcccggcc acgtcatcgg tggcccgctg ccccgccgct
      541 acgacgtcat cgtctccggc ttttctatcc accaccagcc cgacagccgc aaacgacgca
      601 tctacgccga catctatgcc ctgctggctc ccggtggcat cttcctgaac ctggaacatg
      661 tcgcctcggc ctcgcgctgg ggcgaagtcc aattcgagga gtacttcatc gacgcgctgc
      721 accgctttca cgggccgcgg ggcaagacgc gcgaacaggt ggcagccgac ttctaccacc
      781 gccccgacaa gaccgccaac ctgctcagcc cggtggaaac gcaacttaag tggctacgcg
      841 agatcggctt cagccacgtc gattgcttct tcaagatctt tgaactggcc atcttcggcg
      901 gcatccgccc ggccgtggcc gccttcacct tctgcggccc accctttgcc tgattcattc
      961 gccccaactc gagcctctta tacgacctgt cccatgactg ctaaccctca aaccattatc
     1021 gacaagatct ggcagagcca tatcgtcacc gagcagccgg acaccccggc cgtgctctac
     1081 gtcgacctgc acctcatcca cgaagtgacc agcccgcagg cgtttagcgg cctgcgcgcc
     1141 cgcggcctgc ccgtccgccg gccggagcag accgtggcga cgatggatca cagcatcccc
     1201 acgacgccgc tgcacctgcc catcatcgac gtgatggccg cggcccagat cgcccagcta
     1261 gagcaaaact gccgcgactt cggcatcccc ctgcttggct tccacagccc ccaccggggc
     1321 atcgtccacg tcatcggccc ggagctgggc ctgacgcagc cgggcatgac catcgtctgc
     1381 ggcgacagcc acacggccac ccacggcgcg tttggcgcgc tggccttcgg catcggcacc
     1441 agcgaggtcg agcacgtcct agccagccag tgcctcctgc agaacaagcc aaagacctat
     1501 gccgtcaacg tggagggcgc gctgcggcca ggcgtgacgg ccaaggacat catcctggct
     1561 ctgttggcca agatcggcgt cggcgggggc acgggccacg tcttcgagta tcgcggcgcg
     1621 gccattcgcg ctctgtcgat ggaagagcgt atgaccgtct gcaacatgag catcgagggc
     1681 ggcgcgcgag ccggcctcat cgccccggac gagacgacgt tcaactatct ggccgggcgg
     1741 ccgttcgccc cccagggcgc ggcatgggac gcggccgtgg ccggctggcg ggcgttgccg
     1801 tccgacgcgg gcgcgcgctt cgacaagtca gtggacatcg acgccgatgc gctggagccg
     1861 atgatcacct acggcacgaa tcctggcatg ggcctgccca tcaccgcgcc tgtgcccgac
     1921 ccggccgacg ccgccgacct gacccagcgc ctgacgctgc aacgggcgct cgactacatg
     1981 gacttgcagc ccggccggcc gctgttgggc catcccattg atgtcgtctt catcggcagt
     2041 tgcaccaact cgcgcatcag cgacctgcgc caggcggcac agctgatggc cggccggcgc
     2101 gtcaaggacg gcgtgcgcgt gatggtcgtg cccggctcgc aggcggtcaa gcgccaagcc
     2161 gaggccgagg ggttagatcg catcttccgc gccgccgggg ctgagtggcg cgaggcgggg
     2221 tgcagcatgt gcatcgccat gaatggcgac cagttggccc ccggccagta cgccgtcagc
     2281 accagcaacc gcaactttga ggggcgacag ggcaagggcg gacgcacctt cctggccagc
     2341 ccgctaaccg ccgcggccac ggccgtcgct ggcgcaatcg ccgacgcccg cgaactggtg
     2401 tagagtggcg tcggggcggg tctaagaccc gccccgacac gagaaaggaa ggaatccaat
     2461 ggaaccgttc acaaccctga ctggccgcct catagccatc cctaaggccc acatcgatac
     2521 cgaccagatc atcccggccc gctttctaaa gaccatcagc aaggccggcc tggggcagaa
     2581 cctcttcgcc gactggcgct atgacggcga gggacagccc gtggccgact tcccgctcaa
     2641 ccggccggaa cacgccggcg cgcgtatcct gctggccggc gataacttcg gctgcggttc
     2701 cagccgcgag cacgccccct gggcgctggc tgactacggc tttcaggcca tcatcagcac
     2761 atcgttcgcc gacatcttcc gcaacaacgc cctgaagaat ggcctgttgc cagtcatcgt
     2821 cgatgacgac acccacgaac agcttttcag tctggtcggc gaagacccga cgacggagat
     2881 caccatcgac ctggacacgc agtcgctgct gctgcccgac ggccggcgcg tgaccttccc
     2941 catcgacgcc ttcagcaagg tctgcctgct ggaggggctg gaccaactcg gctatctgct
     3001 gaagcacggc ccggccgtcg cggcattcga ggccacccac ccgcagcgcg tcaacacagg
     3061 ggttagggat taggggttag ggattaggat tcctgacgac aactttggtc cactaactca
     3121 ctgacccact ggcccactga cttactgaat actggatact gaccaccgac cactaacaac
     3181 tatgcaaccc atccacctct acgacacgac gctgcgcgac ggcacgcagc gcgagggcat
     3241 ctcctattcg ctggacgaca agcttaagat cgccgcgcgg ctcgacgcct tcggcatgga
     3301 ctacattgag ggtggctggc ccggatcgaa ccccaaggac gtcgagttct tccggcgcgc
     3361 gccgtctctg ggtctgagac acgcgcgcct ggcggccttc ggcagcaccc gccgcaagga
     3421 cacccgcccg gccgacgacg ccaacatcgc cgctttgctg ggcgcggaga cgcccgtcgt
     3481 cacgctcgtc ggcaaaagct gggggctgca cgtcgtcgat gtgctggaga cgacgccggc
     3541 cgagaacctg gccatgatcg gcgacagcgt ggcctattgc gccacccagg gcaaagaggt
     3601 catctacgac gccgagcact tcttcgacgg ctaccgcgcc gacccggcct acgccctggc
     3661 gacgctggag gcggccgccc gcggcggcgc ggcctgtgtc gtcctgtgcg acaccaacgg
     3721 cggcgcgctg ccctgggacg tggaggcgat cgccggccgc gtcattgcac agttagggcc
     3781 gggcgtgccc gtgggcatcc acacccacga
//
LOCUS       DAOSMQ010000308         3807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24106_length_3807_cov_5.046109, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000308 DAOSMQ010000000
VERSION     DAOSMQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24106_length_3807_cov_5.046109"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..594
                     /locus_tag="PK829_10360"
     CDS             16..594
                     /locus_tag="PK829_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOU41656.1"
                     /translation="MFEAISGLSAAFGLSGSAGLNAYIPLLVVALAARFPVSDPLVKL
                     QAPYDLLGSWWAIGLIAVLLVVEIVADKIPAVDSVNDGIQTFVRPAAGAILFAGSANV
                     ITDIHPLLSLAAGLLLAGGVHATKAAARPIVTVSTAGVGNPVVSTLEDIVATVVSLLA
                     VLLPIIAAVLLLSLLLLVIVTIRRWRRRKMVM"
     gene            complement(767..1228)
                     /locus_tag="PK829_10365"
     CDS             complement(767..1228)
                     /locus_tag="PK829_10365"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOU41657.1"
                     /translation="MTNAITVYKLDHAGREVWQYPGRVLARDAGSIRLEGFYNREDTV
                     VSYAVFKRGDRIIETFYSDRWYNVFAVYDRDDGVLKGWYCNVTRPATITDTAVSSDDL
                     ALDVWVSPAGDVTVLDEDEFAALPLPPADRAAAREALAHLLALAGRGELPA"
     gene            complement(1299..3026)
                     /locus_tag="PK829_10370"
     CDS             complement(1299..3026)
                     /locus_tag="PK829_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOU41658.1"
                     /translation="MDAILTQGQEALLKQTRATLGRLRDALADSADPDSRAALVKSIQ
                     QLDELFLLVIAGEFNSGKSAFVNALLGQTLQPEGVTPTTDQIYLLRYGETAQRRPGEN
                     GVWVQTAPVELLSKITVVDTPGTNAIMREHEALTAEFIPRSDLVLFVTSADRPFTESE
                     RGFLEQIRNWGKKIVLVINKIDILSPAEVEQVVAFVTSAAQTLIGDVAAVFPVSARLA
                     QAAKRGRPEQWGPSRFEPLERYITETLDDEGRFRLKLLNPLGVGGRLIGQRLEQLSAD
                     LATLSGDTKLLEDIRGQMVYYDDDMKRNFRARIGEIDNLLLSMEKRGNDFLDEHLRLG
                     RIPDLLRSKQLEQAFQDAVVADTPRQIEDRVNELVDWMVQQDLRQWTAVADHLGKRRD
                     ANLSRVVGDAPREGTMAWDRQRLIDSIGRSTRLAVESYDQTREAADLAISARQAVVNA
                     GLAGVGASIGVIIAVVAHAAFLDFTGVFAGIALAALGVLVLPARKRKSKGEFTAKLTD
                     LRTRLVGNLEQQFERELRRGAQRIEDTIAPFDRFVRAEQARLGQQQTTLQELEAEVAD
                     LRRALQQGG"
     gene            3276..>3807
                     /locus_tag="PK829_10375"
     CDS             3276..>3807
                     /locus_tag="PK829_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU41659.1"
                     /translation="MLDLAAHLNRPVYPASAQPQLVYALLEPRLPRRGASAPVNLALV
                     IDVSASMRIPLLTPQQFEDLAAQGLVQEVIADGIPVWQFENVPTASLQPYPRPIDYVV
                     DALKVVLEQARPADRLSLVAFAARAEMVLPLTAGHDKRRLRPVIRLVESLSLGDDTDM
                     GLGMALSFQELQRGAAP"
BASE COUNT          569 a         1263 c         1263 g          712 t
ORIGIN      
        1 agagagtgaa ttcctatgtt cgaagcgatt agcggcctga gcgccgcttt cggcctgtcc
       61 ggcagcgccg ggctaaatgc ctacattccg ctgctggtag tggccctcgc ggcgcgtttc
      121 cccgtgagcg atccgctggt caagctgcaa gcgccctatg acctgctcgg cagttggtgg
      181 gccattggct tgattgccgt gctgctggtg gtcgagatcg ttgccgacaa gataccggcg
      241 gtcgatagcg tcaacgacgg catccagacc ttcgtgcgcc cggccgcggg ggcgatcctg
      301 tttgccggca gcgccaacgt catcaccgat atccatcccc tcctgtcgct ggccgcgggg
      361 ctgctgttgg ccggcggcgt ccacgccacc aaggcggcgg cgcggccgat tgtgactgtg
      421 tccacagccg gcgtcggcaa cccggtcgtc agcacactgg aggacattgt cgccacggtg
      481 gtgtcgctac tggccgtcct cctgcccatc atcgctgctg tgctgctgct tagcctgctg
      541 ctgcttgtca ttgtcaccat ccggcgctgg cgacggcgca agatggtgat gtaatcgcct
      601 tcatcgttgc tccggccggc gccctgtggg caggctggtt ccaggcaggt tgtctccggc
      661 cgccggccgc gagtgcgcca tggcgactgg actttacatc aacccatttc ttggaggggg
      721 gcggacatcg atccgccctt attgcttttc ctgacccgtt gcgcgcttaa gcaggcagtt
      781 cgccacggcc cgccagcgcc agcaggtggg ccagcgcttc gcgggccgcg gcccggtcgg
      841 ccggcggcag cggcagcgcg gcaaactcgt cctcgtctag caccgtcacg tctcccgccg
      901 gcgacaccca cacgtccagc gccaggtcgt cactgctcac ggcggtgtcg gtaattgttg
      961 ccgggcgggt gacgttgcag taccagccct tcaacacgcc gtcgtcgcgg tcgtagacgg
     1021 cgaagacgtt ataccagcgg tcgctgtaga acgtctcgat gatgcggtcg ccacgcttga
     1081 agacagcata gctcacaacc gtatcctcgc ggttatagaa cccttccaga cggatgctgc
     1141 cggcgtcgcg ggccaggacg cggccagggt attgccacac ctcgcgtccg gcgtggtcga
     1201 gcttgtagac ggtgatggcg ttggtcattg tctggttctc tcgcttccct cccctaaccc
     1261 ctcccgaagg gaggggaacc ggagggcggg gagagatact agcctccctg ctgtaacgcc
     1321 cgccgcaaat cggccacttc cgcctccaac tcttgcaacg tcgtctgctg ctgtcccagc
     1381 cgggcctgct cggcgcggac gaagcggtcg aacggggcga tggtgtcctc gatgcgttgc
     1441 gcgccgcggc gcaactcgcg ctcgaactgc tgctccaggt tgcctaccag ccgggtgcgc
     1501 aggtccgtca gcttggcggt gaattcgccc ttgctcttgc gcttgcgggc gggcaggacg
     1561 agcacgccca aagccgccag ggcaatgccg gcgaaaacgc cggtgaagtc gagaaaggcg
     1621 gcgtgggcga cgacggcgat gatgacgccg atgctggccc cgacgccggc cagcccggca
     1681 ttgacgacgg cctgccgcgc cgatatggcc agatcggctg cctcgcgcgt ctggtcatag
     1741 ctctccacgg ccagccgcgt cgagcggccg atggagtcga tcagccgttg gcgatcccag
     1801 gccattgtgc cctcgcgcgg cgcgtcgccg acgacgcggc tcaggttagc gtcgcggcgc
     1861 ttacccagat ggtcggccac ggctgtccac tggcgcaggt cttgttgcac catccagtct
     1921 accagttcgt tgacgcggtc ttcgatctgc cggggcgtgt cggccacgac tgcgtcctga
     1981 aaggcctgct ccaactgctt ggagcgcagt aggtcgggga tgcgtcccag gcgcaggtgc
     2041 tcgtcgagga agtcgttgcc gcgcttttcc attgacagga ggaggttgtc gatctcgccg
     2101 atgcgggcgc ggaagttgcg cttcatgtcg tcgtcgtaat agaccatctg gccgcgaatg
     2161 tcttcgagca gcttggtgtc gccgctgagg gtggccagat ccgcgctgag ctgctccaac
     2221 cgctggccga tgagccgccc gccgacgccc agcgggttga gcagcttcag gcggaagcgg
     2281 ccctcgtcgt cgagcgtctc ggtgatgtag cgctccagcg gctcgaagcg gctcggcccc
     2341 cactgctccg gccggccgcg cttggccgcc tgcgccagcc gcgcggacac ggggaagacc
     2401 gcggccacgt cgccaatgag cgtctgcgcc gcgctggtga cgaaggcgac gacctgctcg
     2461 acctcggccg ggctgaggat gtcgatcttg ttgatgacca ggacgatctt cttgccccag
     2521 ttgcgaatct gctccaggaa gccgcgctcg ctctcggtga aggggcggtc ggccgacgtg
     2581 acgaacagga cgaggtcgct gcgggggatg aactcggccg tcagggcctc gtgctcgcgc
     2641 atgatggcgt tggtgccggg cgtatcgacg acggtgatct tgctaagtaa ttccaccggc
     2701 gcggtctgca cccagacgcc attctcgccc ggccgccgct gggccgtctc gccatagcgc
     2761 aggagataga tctggtcggt cgtcggcgtc accccctctg gctgcaacgt ctggcccagc
     2821 agggcgttga cgaaggccga cttgccggag ttgaactcgc cggcgatgac cagcagaaat
     2881 agctcgtcca actgctggat cgacttgacc agggcggcgc ggctgtcggg gtcggcgcta
     2941 tcggccaggg cgtcgcgcag gcggccgagg gtcgcgcggg tttgcttgag cagggcctct
     3001 tgaccctgag tcagaattgc gtccatcact ctcccgtttc gataggggat tgtacccgac
     3061 atcgcactgc ggggaaatgc cctctgattg ccaacgagca gcgtgccatt ctggcccgca
     3121 cttgttatca tacggcatag tgcccacagc gaaagtgcct gcgatacaga ttggaaaggg
     3181 gtatctgtga cgctcagtgt cggcgacatc ctcgtcaatc gctaccggat cgtcaagctc
     3241 atcgagcaga gcgggtttgg ggcagtcggc taaccatgct cgacctcgcc gcccacctca
     3301 accgccccgt ctacccggct tccgcccagc cgcaactggt ctacgcccta ctggagccgc
     3361 gcttgccgcg gcgcggcgcg tcggccccgg tcaacctggc cctggtgatt gacgtcagcg
     3421 cctccatgcg catccctctg ctgacgccgc agcagttcga ggatttggcc gcgcagggat
     3481 tggtgcagga agtcatcgcc gacggcatcc ccgtgtggca gttcgaaaac gtccccacag
     3541 cgtccttgca gccctatccc cggccaatcg attacgtcgt tgacgctctc aaggtcgtgc
     3601 tggagcaggc ccgcccggcc gaccgcctgt cgttggtcgc cttcgccgcg cgggccgaga
     3661 tggtgctgcc gctgaccgcc gggcacgaca agcggcggct gcggccggtc atccgcttgg
     3721 tcgagtcgct gagcctgggc gacgacaccg atatgggcct gggcatggcc ctgagcttcc
     3781 aggagttgca gcgcggggcc gcgcccg
//
LOCUS       DAOSMQ010000309         3765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24457_length_3765_cov_4.320216, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000309 DAOSMQ010000000
VERSION     DAOSMQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24457_length_3765_cov_4.320216"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PK829_10380"
     CDS             <1..1328
                     /locus_tag="PK829_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU41660.1"
                     /translation="LYGQPLTARAPGEDVPLTSFGATEFTPSGDGLLLTTGLFEDTYG
                     LATIRPDAPHEIARVEVRGAVHSGAGEMENLVHLRGDRYAVQYNIDGCTWLYEGQFDE
                     ARLTMQLGAVICGQGELAGGVMDGFNYDRQGDCFVLSFSTATSPTQIYTVGGPQRDQV
                     RRHTRERVLGLPAEYLSPGEDASFVSHDGLRVSARLYLPPALGYEGPRPVVYYVHGGP
                     QGQERPDFAWFSMPLIQFLALNGFAVFVPNVRGSTGYGLSYTKHIDRDWGGQDRLDHV
                     HAMKLLADDARVDASRAAVIGRSYGGYMTLTLAARHPELWSAAVDMFGPYDLLTFIDR
                     LPETWKPYFAIACGDPVEDRDFLIERSPRSYITDIQCPLLVIQGRNDPRVIERESRDV
                     VEQLRALGKEVEYLMFADEGHDVLKYENRVTCYNAITAFFRQHLMPAPG"
     gene            1325..2257
                     /locus_tag="PK829_10385"
     CDS             1325..2257
                     /locus_tag="PK829_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOU41661.1"
                     /translation="MNDLVKVYEVGPRDGLQNESTVLSAAQKGRLIDGLVAAGLGHIE
                     VTSFVHPAAVPQLADADEVMRTAVARYGADGIELTGLVFNDRGFDRAAASGCRSLAFG
                     AAVSETFSQRNTHNTPAEALSTARRLVARAQAAGLRTRFYVMTAWICPFEGPVSPHKT
                     MAVVQEIADWGVDEIAVADTIGHADPLSVGRLIDRIARRIDINRLAAHLHDTQALGLA
                     NAAVALQAGIRIFDTSVGGLGGCPFAPGAAGNLATEDIVFMAHKMGLGTGVDLALLWN
                     VVYELERAIGRPIGGRIRQWWESSQDHEPQLGFS"
     gene            complement(2254..3423)
                     /locus_tag="PK829_10390"
     CDS             complement(2254..3423)
                     /locus_tag="PK829_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU41662.1"
                     /translation="MYTIDHLRQTEFPHAATLTYLNHAGIAPLPQRAKRAIQAAVEAL
                     SIDPNDYFGHVGLPLLMELQAGLARFVNAADPAEIAFVTSTSAALNAIAQAIPWQPGD
                     RVLFCDVEFPSNAYPWMGLQRDGVESCIVPADNGGLTLAAVEQHADERTRVIAASAVQ
                     FFTGHRTDLAAIGQFCRQRGILFVVDAIQAIGHMPFDVRAMNIDVLATGGQKSLLGLP
                     GVGFIFVRDEAAQAMRPRLIHSNATTDYLHWLAYDLTPLPGAARFNAGTPNVAGIIGL
                     GPSLALLQELDVANIDAHTTALSRYACERLSAAGLRVITPSDALGPIVTFRSPVDAAA
                     TETIVQRLAAERVIVVKHLDAAGAAYIRLSTHCYNVREDIDRFVEAYQRLSAASG"
     gene            3490..>3765
                     /locus_tag="PK829_10395"
     CDS             3490..>3765
                     /locus_tag="PK829_10395"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOU41663.1"
                     /translation="MDLSPDVLLSAYMQGIFPMAHENGRIYWYSPDPRAILPLDGLHV
                     SQSLRRVIHRGAFDVRFDTAFDAVIAACATPAPGREQTWISAEIQRAY"
BASE COUNT          615 a         1203 c         1294 g          653 t
ORIGIN      
        1 tcctctatgg ccagcccctg accgcgcgcg cgccgggcga ggatgtgccg ctcaccagct
       61 ttggcgcgac cgaattcact ccctcgggcg acggcctgct gctgacaacc ggtctgtttg
      121 aggacaccta tgggctggcc accatccggc cggacgcgcc gcacgagatc gcccgtgtgg
      181 aagtgcgtgg cgccgtccac tctggcgcgg gcgaaatgga gaacctggtg cacctgcgcg
      241 gcgaccgcta tgcggtgcaa tacaatatcg acggctgcac atggctctat gagggccagt
      301 tcgacgaggc caggttgaca atgcaactgg gcgcggtcat ctgtggccag ggcgagctgg
      361 ccggcggggt gatggacggc ttcaactacg acaggcaggg cgattgcttc gtcctcagct
      421 tctccacggc aacttcgccg acgcagatct acaccgtcgg ggggccgcag cgcgaccagg
      481 tgcgtcgcca cacgcgcgag cgcgtcctcg gcctgccggc ggagtatctg tcgccggggg
      541 aagacgcgtc gtttgtctcc catgatggct tgcgcgtttc ggcccggctc tacctgccgc
      601 cggcgctggg ctatgagggg ccgcggccgg tggtctacta cgtccacggc ggcccgcaag
      661 ggcaggagcg gccggatttc gcctggttct ccatgccgct gatccagttc ctggcgctca
      721 acggctttgc cgtcttcgtg cccaacgttc gcggcagcac cggctatggc ctcagctaca
      781 ccaagcacat cgaccgcgac tggggcgggc aagaccggct ggatcacgtc cacgccatga
      841 aactgctggc cgacgatgcg cgcgtcgacg cctcgcgggc ggcagtcatt ggccgctcct
      901 acggcggcta tatgaccctg accttggccg cgcgccaccc ggagttgtgg tcggcggccg
      961 tggatatgtt cggcccctac gatctgctga cgttcatcga ccggctgccg gaaacgtgga
     1021 agccctactt cgccatcgcc tgtggcgacc cggtggaaga ccgcgacttc ctgatcgagc
     1081 gctcgccgcg cagctacatc accgacatcc agtgcccgct gctggtcatt cagggacgca
     1141 acgacccgcg ggtgatcgag cgcgagtcgc gcgacgtcgt tgaacaactg cgtgccctgg
     1201 gcaaggaggt cgaatacctg atgttcgccg acgaagggca cgacgtcctc aaatacgaga
     1261 accgggtgac ctgctacaat gccatcaccg ctttctttcg ccagcatctc atgcctgcgc
     1321 caggatgaac gatctcgtca aggtctacga ggtcggtccc cgcgacggct tgcagaacga
     1381 atcgacggtt ctgagcgcgg cgcagaaggg gcggctgatc gatgggctgg tggccgccgg
     1441 cctgggccac attgaagtca ccagtttcgt gcacccagcg gccgtgcctc aactggccga
     1501 tgccgacgag gtcatgcgca cagccgtcgc ccgttacggc gcggacggca tcgaactgac
     1561 cgggctggtc ttcaacgacc gcggcttcga ccgggccgcg gccagcggtt gccgctcgct
     1621 ggcctttggc gcagctgtca gcgagacctt tagccagcgc aacacccaca acacccccgc
     1681 cgaggccctg tctacggccc gtcgtctcgt cgcgcgggcg caggcggccg gcctgcgcac
     1741 gcgcttctac gtgatgacgg cctggatttg cccgtttgag gggccggtgt cgccacataa
     1801 gacaatggcc gtggtgcagg agatcgccga ttggggtgtg gacgagatcg ccgtggccga
     1861 caccattggc cacgccgacc cgctctcggt cggccggctc atcgaccgca tcgcccggcg
     1921 catcgacatc aaccggctgg ccgcccatct acacgacact caggcgctgg gcctggccaa
     1981 tgccgccgtg gctctgcaag cgggcattcg catcttcgat accagcgtcg gtgggctggg
     2041 cggctgcccg ttcgctcctg gcgcggccgg caacctggcc actgaggaca tcgtcttcat
     2101 ggcccataag atggggctgg gcacgggcgt ggacttggcc ctgctgtgga acgtggtcta
     2161 tgagctagag cgcgccatcg gccggcccat cggcgggcgt atccgccagt ggtgggaaag
     2221 cagccaggac cacgagccac agctcggctt cagctaacct gacgcggcgc tcaaacgctg
     2281 atacgcctcg acaaagcggt cgatgtcctc gcggacgttg tagcagtggg tcgagaggcg
     2341 aatgtaggcc gcgccggcgg catcgagatg cttgacgacg atgacgcgct cggcggccag
     2401 gcgctggacg atggtctcgg tcgccgccgc gtccaccggc gagcggaagg tgacgattgg
     2461 ccccagcgcg tcggacggcg tgatgacgcg caggccagcg gccgacaacc gttcgcaggc
     2521 gtagcggctg agggccgtcg tgtgggcgtc gatattggcc acgtccaact cttgcagcag
     2581 cgccaggctc ggccccaggc cgatgatgcc ggcgacgttg ggcgtgccgg cgttgaaccg
     2641 cgccgcgccc ggcaatggag tcaggtcgta ggccagccag tgcagatagt cggtcgtggc
     2701 gttggagtgg atgagccgcg ggcgcatggc ctgggccgct tcgtcgcgga cgaagatgaa
     2761 gccgacgccc ggcagaccca gcagcgactt ctgaccgcct gtggccagca cgtcgatgtt
     2821 catggcccga acgtcaaaag gcatgtggcc gatagcctgg atggcgtcga cgacgaagag
     2881 gatgccgcgc tggcgacaga actggccaat ggcggccagg tcggtgcgat ggccggtgaa
     2941 gaactgcact gcgctggcgg cgatgacgcg ggtacgttcg tcagcgtgct gctcgactgc
     3001 tgccagcgtc aggccgccat tgtcggccgg gacgatgcag ctctcgacgc catcgcgctg
     3061 gaggcccatc cacgggtagg cgttagatgg gaactcgaca tcgcagaaca ggacgcggtc
     3121 gcccggctgc caggggatgg cctgggcaat ggcgttcagg gcggcgctgg tgctggtgac
     3181 gaaggcaatc tccgcgggat cggcggcgtt gacgaaccgg gccagcccgg cctgcaattc
     3241 catgagcagc ggcaggccga catggccgaa gtaatcgttg gggtcgatac tgagcgcctc
     3301 gacggccgcc tgtatcgcgc gcttggcccg ctggggcaaa ggcgcgatgc cggcatggtt
     3361 gaggtaggtc agagtggcgg cgtgtggaaa ttcagtctgg cgaaggtggt caatcgtgta
     3421 catgtgggtt ctgtccatga tggtgctgat aaccctggat tatagtgcta ctgcctgtgg
     3481 ggccaatgaa tggatctgag tcctgatgtg ttgctgtcgg cctacatgca gggcattttc
     3541 ccgatggccc atgagaacgg ccgcatctac tggtactcgc ccgacccacg ggctatcctg
     3601 cccctggatg ggctacacgt gtcgcaatcg ttgcgccgcg tcatccatcg gggcgcgttt
     3661 gatgttcgct tcgacacggc gttcgatgcg gtgatcgctg cctgcgccac gccggcccct
     3721 ggccgtgagc agacgtggat cagcgctgag attcagcggg cctac
//
LOCUS       DAOSMQ010000310         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24571_length_3751_cov_4.570346, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000310 DAOSMQ010000000
VERSION     DAOSMQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24571_length_3751_cov_4.570346"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..821
                     /locus_tag="PK829_10400"
     CDS             225..821
                     /locus_tag="PK829_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOU41664.1"
                     /translation="MSVRNAILGLLADRPRHGYDLHAAFEAIAGGEALWALKPAQVYS
                     TLARLEEGGLVAQESIEQDGGPEKHIYSLTPAGEAELAAWFASGVPTEHQRDEFFVKL
                     MLSLGNDTTDPYRVIQAQRNRLYQELHDATARRNRADARVELAQIFLLDKSIMHLEAD
                     VRWLDLIEARLDDIRRQPLPKPQPRPRGRPRKKLGIRN"
     gene            906..1658
                     /locus_tag="PK829_10405"
     CDS             906..1658
                     /locus_tag="PK829_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41665.1"
                     /translation="MSLISAVNLNKVYGSGDTAVTALKGVSLQVEPGEFMAIMGPSGC
                     GKSTLLHLLGGLDRPTSGTVMIDGHNLSDLPDNKLTEIRRRRIGFIFQFFNLIPVLNA
                     VENTALPITLDGVRPGEAKARATEWLRKIGLGDRLDHRPDQLSGGQQQRVAVARALVA
                     DPALILADEPTGNLDTRASEEIAALLRQVANEWGRAVVIVTHDPRIAAYADRIIFLKD
                     GAIVDQTVLEPRSYDNGQGAEMVAGKLQAMGD"
     gene            1661..>3751
                     /locus_tag="PK829_10410"
     CDS             1661..>3751
                     /locus_tag="PK829_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU41666.1"
                     /translation="MNIQLTLAARYLRGRKLRTALTTLAIFFGVLVLFGMNTLLPAFT
                     RAFQGNILAAAGQVDATVTLKTGDAFDAAVADRVAGVEGVRAVSRLLDRTINLPVDYL
                     DNDPALPDPATALSLVGLDVAQATALHAYQVSDGRFLEAGDTDATVISRSLAELLGLG
                     VGDGLTLPTPTGEQTLTIVGLLPPRTQLGDEEVLVTLPQAQAMLAMPGLINAVETNFE
                     PVNEAERAAVEDAILAELGDTFQLGALSSNIELLANLTVAQGIFTLLGVLALLMGGFI
                     IFNTFRTVVTERRRDIGMLRTLGASRRTILGTFLAEGLIQGVVGTALGLLGGYGLGWL
                     GVTALAPLMSRMIHLDIGAPVVSPALVIGSVIVGVGVTLLAGLLPAISAGRVTPMEAL
                     RPAIGAVSLRRLTGVGFWAGVVMLGLAIAALVTRNVSVIALGAVLFIAGLVLIAPVLV
                     TPVARFFSGLLAAVFARSGTAQLAEGNLTRQPSRAAITASTMLIGIAILIMAASIIGS
                     VTIGFSNVLRKSLGSDYIFVPPSIAVWGSDVGSGAELADSLRAVEGVGLVTTLRFAPT
                     QVDDTAVSLLGIDPATYPQVSGLTFSQGDSAAYDALLDGRGIIVNPLLSAQSGAELGQ
                     SVELVTPTGTQTYTVVGVGGDYLNAKLATAYISQANLATDFNRTEDVLLQVNLAEGAD
                     REAAEAGMQAAIRNY"
BASE COUNT          633 a         1273 c         1204 g          641 t
ORIGIN      
        1 tgtggaggat catggactgg tagtatgtgc ctgcgtgggg aaatgtgcaa gttgtgagtg
       61 gagggcaggg gagcagggga gcgagggagc gggggaggtg cgtcgcactc cagaaagtgc
      121 caggcactaa ggagcctctt gacaaaacta gtaactcgtt atatatttat cgtcgtaaat
      181 agcaatcaag ttagtagttt atttggccgt cgtttttgac tgttatgtcc gtccgcaatg
      241 ccatcctggg cctactggcc gaccgccccc gccacgggta cgacctgcat gccgcctttg
      301 aggccattgc cggcggcgag gcgctgtggg cgctgaaacc ggcgcaggtc tacagcaccc
      361 tggcgcggct ggaagagggc ggcctggtgg ctcaggagag catcgagcag gacggcggcc
      421 cggagaaaca catctacagc ctgacgcccg cgggcgaggc cgagctggcc gcgtggtttg
      481 ccagcggcgt gcccaccgag caccagcgcg acgagttctt cgtcaagctc atgctcagcc
      541 tgggcaacga caccaccgac ccctaccgcg tcatccaggc ccagcgcaac cggctctatc
      601 aggagcttca cgacgccacc gcgcgccgca accgcgccga cgcccgcgtc gagctggcgc
      661 agatctttct gctcgacaaa agcattatgc atctggaagc cgacgtgcgt tggctggacc
      721 tcatcgaggc ccggctcgac gacatccgtc gccaacccct gcccaagccc cagccgcgtc
      781 cgcgcggaag gccgaggaag aaattaggaa ttaggaattg agaattagga attaagagtt
      841 ggggaggtcg agagaacgac attagacagc cggaattctt cgattcctaa ttctacggag
      901 caatcatgtc cctcatatcg gcggtcaatc tgaataaggt ctatggttcg ggcgatacgg
      961 cggtgacggc gctcaaaggc gtctcgctgc aagttgagcc aggtgagttt atggccatca
     1021 tggggccgag cggctgcggc aagtcaacgc tcctgcacct gttgggcggc cttgaccggc
     1081 cgaccagcgg tacggtgatg atcgacggcc acaacctgtc cgacctcccc gacaacaagc
     1141 tgacggaaat ccgccggcgg cgtatcggct tcatcttcca gttcttcaac ctcattccgg
     1201 tgctcaacgc tgtcgagaac acggccctgc ccatcaccct cgacggcgtg cggccgggcg
     1261 aagccaaagc ccgcgccacc gaatggctgc gcaagatcgg cctgggcgac cgccttgatc
     1321 accggcctga ccagctctcc ggcggccagc agcagcgcgt cgccgtggcc cgcgccctcg
     1381 tggccgaccc ggctctcatt ctggccgacg agccaacggg caacctggac acgcgcgcct
     1441 cggaggagat cgccgcgctg ctacgccagg tggctaacga gtggggccgg gcggtcgtta
     1501 tcgtcaccca cgatccgcgc atcgccgcct acgccgaccg gattatcttt ctgaaggacg
     1561 gtgctatcgt cgatcagacg gtcctggaac cgcgcagcta cgacaacggc caaggggccg
     1621 agatggtggc cggcaagctc caggcgatgg gagactagcc atgaacatcc aattgacgct
     1681 cgccgcacgc tatctgcgcg gacgcaagct gcgcacggcc ctgacgacgc tggccatctt
     1741 ctttggcgtg ctcgtcctgt ttggcatgaa cacgctcctg ccagccttta cacgtgcctt
     1801 ccagggcaac atcctggctg ccgccggcca ggtcgacgcg accgtgaccc tcaagacggg
     1861 tgacgccttc gacgccgctg tcgccgaccg cgtcgccggg gtcgagggcg tgcgcgccgt
     1921 ctccaggctg ctcgaccgga cgatcaacct gccggttgac tacctcgaca acgatcccgc
     1981 cctgcccgac ccggccacgg ctctgtcgct ggtgggtctg gacgtagccc aggccacggc
     2041 cctgcacgcc tatcaggtct ccgacggccg tttcctcgaa gcgggcgaca ccgacgcgac
     2101 cgtcatcagc cgtagcctgg ccgaattgct gggtctgggc gtgggcgacg ggctgacgct
     2161 gccgacgccc acgggcgagc agacgctgac catcgtcggc ctgctgccgc cacgcaccca
     2221 actgggcgac gaagaagtcc ttgtcaccct gccccaagcc caggcgatgc tggccatgcc
     2281 cggcctcatc aacgcggtcg agaccaactt cgagcctgtc aatgaggccg aacgcgcggc
     2341 tgtcgaggac gccatcctgg ccgaactggg cgacaccttc caactgggcg cgctgtcgtc
     2401 gaacatcgaa ctgctggcca acctcaccgt cgcccagggc attttcaccc tcctgggcgt
     2461 gctggccttg ctgatgggtg gcttcatcat cttcaacacc ttccgcaccg tcgtgaccga
     2521 gcgccgccgc gacatcggca tgctgcgcac cctgggggcc agccgtcgca ccattctggg
     2581 cacattcctg gccgaggggc tgatccaggg cgtggtcggc acggcgctgg gcttgctggg
     2641 cggctatgga ctggggtggc tgggcgtcac ggctctagcg ccgctgatga gccggatgat
     2701 ccatctcgac atcggcgcgc cggttgtctc ccccgcgctg gtcattggca gcgtcatcgt
     2761 cggcgtcggc gtgacgctgc tggccgggtt gctgccggcc atcagcgccg ggcgcgtgac
     2821 gccgatggag gccctgcggc cggcgattgg cgcggtctcg ctgcggcgac tgaccggcgt
     2881 cggcttctgg gcgggcgtgg tcatgctcgg cctagccatc gccgcgctgg tgacgcgcaa
     2941 cgtaagcgtc atcgccctgg gggcggtgct gttcatcgcc ggcctggtgc tcatcgcccc
     3001 cgtgctggtg acgccggtgg cccgcttctt cagtggcctg ctggcggccg tctttgcccg
     3061 cagcggcacg gcccagctgg ccgagggcaa cctcacccgc cagccatcgc gcgcggccat
     3121 taccgccagc accatgctca tcggcatcgc cattctgatc atggccgctt cgattatcgg
     3181 cagcgtcacc atcggcttta gcaacgtcct gcgcaaaagc ctgggcagcg actacatctt
     3241 cgtgccaccg tccattgccg tctggggcag cgacgtcggc tccggggcag aactggcgga
     3301 cagcctgcgc gccgttgagg gcgtgggcct ggtcactacg ctgcgcttcg ccccgacgca
     3361 ggtcgacgat acggccgtct cgctgttggg catcgacccg gcgacctacc cgcaggtcag
     3421 cggcctgacc ttcagccagg gcgacagcgc ggcctatgac gccctgctgg atggccgcgg
     3481 gatcatcgtc aaccccctgc tcagcgccca atccggcgcg gagctgggcc agtccgtcga
     3541 actggtgacg cccaccggca cgcagaccta cacggtcgtg ggcgtcggcg gcgactacct
     3601 caacgccaag ctcgccacgg cctatatctc ccaggccaac ctggccaccg acttcaaccg
     3661 caccgaggat gtgctcctgc aagtcaatct ggccgagggc gcggaccggg aggcggccga
     3721 ggcgggcatg caggcggcta tccgcaacta t
//
LOCUS       DAOSMQ010000311         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24576_length_3751_cov_3.714286, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000311 DAOSMQ010000000
VERSION     DAOSMQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24576_length_3751_cov_3.714286"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PK829_10415"
     CDS             <1..972
                     /locus_tag="PK829_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOU41667.1"
                     /translation="EEAPLRLRPGDRMGEVEIIQWLATLPVATLYKARRGEAIVLLKV
                     AHPGYEDKLKREARFLAANSHPALPQLLPALRGLPAQQHPYGKAATHGRIVYFVVFAF
                     VEGQMLREMLDRNSQPWYLTTGWLIAQLADGLAFLNDGGLLHLCLSPEMVLVRFDAED
                     IPRPLLTDLGVACAYEDIPRYWRSPYASPLYAAPELVRGEGVGAAADVYGIGGLLFEM
                     LAGHAQVRGVASTADELPALATADEALALRTDLTALPELAARAVALDWRRRPAGLRAF
                     WQELMTALPPVPQENHGRAFRAEALRMLAIVALVLVLLLVLALLTPG"
     gene            complement(969..1139)
                     /locus_tag="PK829_10420"
     CDS             complement(969..1139)
                     /locus_tag="PK829_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41668.1"
                     /translation="MAERFIADLNAAAAAVRASLAAKGSMAPVYGLATALPFRGSGSD
                     ILKRSVDRLYKV"
     gene            1256..>3751
                     /locus_tag="PK829_10425"
     CDS             1256..>3751
                     /locus_tag="PK829_10425"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOU41669.1"
                     /translation="MQSVFDLTQPRDEVLHGQLSEEMFAARLKDVIDGRAEPVYADPD
                     RFFGYTYPTAGLQTLLREAIGRLTGQSPTNNPIIRLETSFGGGKTHNLIALYHAARGH
                     VAPEVAARFLGDVALPPPDRVLVTGIVASDLDPMNGVAHPEDNLTTYTLWGELAYQLG
                     GRAGYALAEASDRERVAPGTGVFERLVGARPTLIMLDEIAAALRKGSAVGARVGKSTL
                     AEQTTAFLMALMEFVASQRQVVLVLTLASEEDAFSKETATLREALRISARQERVLTPA
                     AENEISSIVAHRLFTRIDREAASEVIGRYGDYYRELERQDAPIHDRAGRADYLTEFAL
                     SYPFHPELIRALNLKVATIPNFQRTRGALRLLAGVVRRLWQQRPADTWLIHPYHVDLG
                     DVDIVEELTGRLDRAKFKQVAEADIVSFQAGIPAHAAHADETLVAAGRPPYARRLGTT
                     IFLHSLTQGIASGVELPELTLATVTPGESGGDDPALVQRALERLVAQAWFLEFDGFRY
                     RFKTEPSLNKIIDDETALVTTTRAKGEIDSRIRAIWRSGFLKPIYFPATPADVDDDAG
                     KPKLVILHFDALKLRAAETDPPDLLQRIAQYAGQSESFRTYQNNVLFLVADADQVDTM
                     LGEVRRYLAIDRITGSADRMRSFNEEQQKKLREMKGAAELQVRIAITRAYRYLFYPGP
                     DAPKAHAFLRRENVPPQAQGETDQDQTNVIVRVLHTYKKVQAADDSPLAGAYVKSRAW
                     DRTQTEMTTEDLRRAFARKINLPLLLDSSQLRKTIESGVKTGAWVYYDSRAEVAYDRD
                     APPPATEIGEHTWLYEPEEAARLGLLKPPSLTGR"
BASE COUNT          616 a         1356 c         1164 g          615 t
ORIGIN      
        1 gaagaagcgc cgctgcgtct gcgccccggc gaccgcatgg gcgaggtaga gatcatccag
       61 tggctcgcca cgttgcccgt ggcgacgctc tacaaggcac gacgcggcga ggcgatcgtc
      121 ctcctcaaag tggcccaccc cggctatgag gacaaactca agcgcgaggc gcgcttcctg
      181 gcggccaaca gccacccggc gctgccccaa ttgttgccag ccctgcgcgg cctgccagcc
      241 caacagcacc cctatggcaa agcggcgacg cacggccgca tcgtctattt cgtcgttttc
      301 gcctttgtgg aggggcaaat gctgcgcgag atgctggacc gcaattccca gccctggtat
      361 ttgacgacgg gctggctcat tgctcaactg gccgatgggc tggcttttct gaatgacggc
      421 gggctgctgc acctatgcct ctcgccggag atggtactgg tgcgtttcga tgccgaggac
      481 atcccgcgac cgctgctaac cgatctgggc gtcgcttgcg cctatgagga tattccccgt
      541 tactggcggt caccctacgc ctcgcctctc tacgccgcgc cagaactcgt caggggcgag
      601 ggcgtcggcg cggcggccga tgtttatggc atcggcggtc tcctcttcga gatgcttgcc
      661 ggccacgcgc aagttcgcgg cgtggccagc acggccgatg aactgccagc gctggcgacc
      721 gcggacgaag ccctggccct gcgcactgac ctgacggcgc tgccagagtt ggcggcgcgc
      781 gccgtcgctc tcgattggcg acgccggccg gccggcctgc gcgctttctg gcaagaactc
      841 atgacagcgt tgccgcctgt cccacaggag aaccatggcc gcgcctttcg tgccgaggcg
      901 ctgcgcatgc tggccattgt ggctctggtg ctcgtattgc tattggtttt ggcgttgctg
      961 acgcccggct agaccttata gagcctatcg acggagcgtt tcaggatgtc gctgccgctg
     1021 ccgcgaaacg gcagcgccgt cgccaggcca tataccgggg ccatgctccc tttggccgcc
     1081 aggctggccc ggacggcagc ggcggccgcg ttcagatcgg cgatgaaccg ttcggccacg
     1141 accggctgcg tgtgccgcag cccggtcgtc cactcgcttc gcttgccctc cttcccgtcc
     1201 gcggcataat agcggcgcta cacgaccact ctcccgccat aataaaggac gacccatgca
     1261 gtctgtcttt gatctcaccc aaccacgcga cgaagtgctc cacggccaac tgagcgagga
     1321 gatgtttgcc gcccgtctga aagacgtcat cgatggccgc gctgagccgg tctatgctga
     1381 ccctgaccgc ttcttcggct atacctatcc caccgctggc ctgcaaactc tcctgcgcga
     1441 ggccatcggc cgcctcaccg gccagtcgcc caccaacaac cccatcatcc gtctcgaaac
     1501 ctcgttcggc ggtggcaaga cccacaacct catcgctctc taccacgctg cccgcggtca
     1561 cgtcgcgcca gaggtggcgg ctcgcttcct cggcgatgtg gccctacccc cacccgatcg
     1621 tgtcctcgtc accggcatcg tcgcctccga cctagacccg atgaacgggg tcgcccaccc
     1681 cgaagataac ctgacgacct acacgctgtg gggcgagctg gcctatcaac tgggcggccg
     1741 cgccggctac gccctggccg aagcctccga ccgcgagcgc gtcgcgccgg gtaccggtgt
     1801 cttcgagcgc ctcgtcggcg cgcggccaac gctgatcatg ctcgacgaga tcgccgccgc
     1861 gctgcgcaag ggcagtgccg tcggcgcgcg cgtcggcaaa tcgaccctgg ccgagcagac
     1921 gaccgccttt ctcatggcgc tcatggagtt cgtcgccagt cagcgccagg tcgtgctggt
     1981 gctgaccctg gccagcgagg aggacgcctt cagcaaggag acggccacgc tgcgcgaagc
     2041 cctgcgcatc tctgcccggc aggagcgcgt cctgaccccg gcggccgaaa acgagatttc
     2101 ttctatcgtc gcccaccgcc tcttcacccg catcgatcgc gaggccgcgt ctgaggtcat
     2161 cggccgctat ggcgattact accgcgagct ggagcgacag gacgcgccca tccacgaccg
     2221 cgccggccgc gccgactacc tgaccgagtt cgccctgtcc tacccgttcc acccggagct
     2281 gatccgcgcc ctcaacctga aggtcgccac catccccaac ttccagcgca cgcgcggcgc
     2341 gctgcggttg ttggccggcg tcgtgcgtcg cctctggcag cagcgcccgg ccgatacctg
     2401 gctcatccat ccctatcacg tcgacctggg cgacgtcgac attgtcgagg agctgaccgg
     2461 ccggctggac cgggccaagt tcaaacaggt ggcggaggcc gacatcgtca gcttccaggc
     2521 tggcatcccc gcccacgcgg cccacgccga cgagacgctg gtcgccgccg ggcggccgcc
     2581 ctacgcccgc cggctgggca cgaccatctt tctccacagc ctgacccagg gtatcgccag
     2641 cggcgtcgaa ttgccggagt tgacgctggc cacggtgacg ccgggcgaga gcggcggcga
     2701 cgacccggcc cttgtccagc gggcgctgga gcggctggtg gcccaggcct ggttcctgga
     2761 gttcgacggc ttccgctacc gctttaagac cgagccgtcg ctcaacaaga tcatcgatga
     2821 tgagacggcg ctggtgacga ccacgcgggc caagggcgag atcgacagcc gcatccgcgc
     2881 catctggcgc tccggctttc tgaagcccat ctacttcccg gccacgccgg ccgacgtaga
     2941 cgacgacgcc ggcaagccga agctggtcat cctgcacttc gacgccctca agctccgcgc
     3001 tgccgagacc gacccgcccg acctgttgca gcgcatcgcc cagtatgccg ggcagagcga
     3061 gtcgtttcgc acctaccaga acaacgtcct ctttctagtg gccgacgccg accaggtgga
     3121 cacgatgctg ggcgaggtgc gccgctacct ggccatcgac cgcatcaccg gcagcgccga
     3181 ccggatgcgg tcgttcaacg aggagcagca gaagaagctg cgcgagatga aaggcgcggc
     3241 cgagttgcag gtgcgcatcg ccatcacccg cgcctatcgc tacctctttt accccggccc
     3301 cgacgcgccc aaggcccacg ccttcctgcg gcgcgagaac gtgccgcccc aggcccaggg
     3361 cgagacagac caggaccaga ccaacgtcat cgtccgcgtg ctccatacct ataagaaggt
     3421 tcaggcggcc gacgattcgc cgctggccgg ggcctacgtc aagtcgcgcg cctgggaccg
     3481 cacccagacg gagatgacca cggaagacct gcggcgcgcc tttgcccgca agatcaacct
     3541 gccgctgctg ctcgactcta gtcagttgcg gaagacaatt gagagtggcg tgaagaccgg
     3601 ggcgtgggtc tactacgaca gtcgcgccga ggtggcctac gaccgggacg cgccgccccc
     3661 cgccacggag atcggcgagc acacctggct gtatgagccg gaggaagcgg cgcggctggg
     3721 gttgctgaag ccaccctctc tcacagggcg a
//
LOCUS       DAOSMQ010000312         3737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24686_length_3737_cov_4.095329, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000312 DAOSMQ010000000
VERSION     DAOSMQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24686_length_3737_cov_4.095329"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2369
                     /locus_tag="PK829_10430"
     CDS             <1..2369
                     /locus_tag="PK829_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOU41670.1"
                     /translation="FLNGVTLSPTFPPLDPWLSGYGISYYYFGYVMTSLLARLAVVPT
                     AVAFNLAIAWLVAGAAVGAFGVVYNLVAGGGIRNYELGIRDSEERRVAPGSSYAPAIK
                     RRAITLGIIAALAVPVAGNMQIGLEVLHGNGVGSDSFWQWLNVRDINGPADSDGVPRY
                     QTSEWWWWRTSRVIHEYTLAGRAEEGLEPIVEVPAFSYVLGDLHPHVLALPFAFLSLG
                     VALAWWLRLTIRNDELGIRNEKEGRPGWLRGTIRNEEGIGPGWPLWLLTALVLGGLSF
                     LNTWDVGIHLFVVLGAFFLAGWRRQGHTSGLWRQTALMAVLLVAAAFVFYLPFYLGFR
                     SQAGPPFLLPMLMRPTRLAHYAIIFGLPLVAITVLLVTLAARQRFRHWQTGLGAVAAV
                     VGGLFLVMLLLLWVVAASQSSSGTISNLAGELGLSLPARPEGAVALGWGLRAAVAVLP
                     AWLAARAVSPGLLLLLSGLLALVVMALRSAVEEASPLAPRQRVEERGNLAPSSLAPWE
                     RVRERGHLAWAEHATLPFALLLILTGVLLTLGPEFVYLRDNFGVRLNTTFKFYYQAWA
                     MFGVAAVFALDYLLATRQQGSQRLVIVGTTAVYVALLAVALLFPFYAVQSRAAEYRGP
                     ARDSAGARLERQPATLDGLAALQRANPSEYEALAWLRNNAVVPGEAPPVVLEAVGGQY
                     SAFGRVAANTGLPTVLGWAGHEWQWRGSDHPEPGRRGPLVEQIYTTPDLNAVAFLLDE
                     LNVRYIYVGDLETSTYGGVGLEKFRDRLEVAFANDRVTIYRWQPGSPN"
     gene            2376..>3737
                     /locus_tag="PK829_10435"
     CDS             2376..>3737
                     /locus_tag="PK829_10435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU41671.1"
                     /translation="MSEGLAETQETRVAALPAGPRPSANGRPAAPRTWRTALTVADGI
                     MLLIVGIAAVARLVNLGAQPLSPTEAEAALAGWQFAHGIPLTAAPASPAYFTLTGLLM
                     SLGGDGDTAARLAPALFGILTVALPWLWRGRFQPAVWLTAGVLLAFSPIQMILSRTAG
                     GDAIALFALLLLAVTAARLDEGDGWGVAAGAALGLGLTTAPLFYTGLVAFLPAWWVFR
                     GAESVGRGTLHAGALAAVIAFITVAAGGLFYPEGLGGALMLLPQWLGQFGLAGGAAAR
                     EPLLVLLRYELGLFLLGIPAAVGALSGDNNVGKQLVLWLGLLLPVILLQMALPHQAAA
                     VLVPGYLLIGLLAGDLMRGELPRTEQRRAWLVGGGLALLGMTLLVSIARFTQLGLWTG
                     SNASLISLAVLAFALAGVAVIVVLAWESPAARRGAFLAVVAMLLYWQWGTGWHLSQAA
                     ANDP"
BASE COUNT          474 a         1222 c         1357 g          684 t
ORIGIN      
        1 ccttcctcaa cggcgtcacc ctcagcccaa ctttcccgcc gctcgacccg tggttgtcgg
       61 gctatggcat cagctactac tacttcggct acgtcatgac ctcgctgctg gcccggctgg
      121 cggtcgtgcc gacggcggtg gcgttcaatc tggccatcgc ctggctggtg gcgggggcgg
      181 cggtgggcgc gtttggggta gtttataacc tggtggctgg tgggggaatt aggaattacg
      241 aattaggaat tagggattcg gaagagaggc gggtcgcacc tggcagcagc tacgcgccag
      301 ccatcaagcg ccgggcgatc accctgggca tcatcgccgc cctggccgtg cccgtagccg
      361 gcaacatgca gatcggcctg gaagtgctgc atggcaacgg cgtcggctca gacagcttct
      421 ggcaatggct gaacgtgcgc gacatcaacg gcccggccga cagcgacggc gtgccgcgct
      481 accagaccag tgagtggtgg tggtggcgca cctcgcgcgt catccatgag tacaccctgg
      541 ccggccgggc cgaggaaggg ctggagccaa tcgtcgaagt gcctgccttc agctatgtgc
      601 tgggcgactt gcacccccac gtgctggcgc tgccgttcgc cttcctgagc ctgggcgtgg
      661 ccctggcgtg gtggctgcgg ctgacaatca ggaatgacga attaggaatt aggaatgaaa
      721 aggagggccg gccgggctgg ctgaggggga caattaggaa tgaagagggg attgggccgg
      781 gttggccatt gtggctgctg acggcgctgg tgctgggcgg gctgtcgttt ctgaacacct
      841 gggacgtggg catccacctg ttcgtcgtcc tgggggcatt cttcctggcc ggctggcggc
      901 gacagggcca cacgtccgga ctgtggcgcc aaacggcgct catggccgtg ctgctggtgg
      961 ccgcggcctt cgtgttctac ctgccctttt acctgggctt ccgctcgcag gccgggccgc
     1021 ccttcctgct gccaatgttg atgcggccga cgcgcctggc ccactacgcc attatcttcg
     1081 gcctgccgct ggtggccatc accgtcttgc tggttacgct ggcggcgcgg cagcgcttcc
     1141 gccactggca aaccggactg ggcgcggtcg cggccgtcgt cggcgggttg ttcctggtga
     1201 tgctgcttct gctgtgggtc gtcgccgcca gccagagcag cagcggcacg atcagcaacc
     1261 tggccggcga gttggggctg agcctgcccg cgcgaccgga gggggcagtc gccctcggct
     1321 gggggctgcg ggcggccgta gcggtgttgc cggcgtggct ggcggcgcgg gcagtgtcac
     1381 cggggctgct gctgctgctc tccggcctgc tggcgctggt ggtcatggcc ctgcgctcag
     1441 ccgtcgagga agcttctccc ctcgcccccc ggcagagggt tgaggagagg ggcaacctcg
     1501 ccccttcttc cctcgccccc tgggagaggg ttagggagag gggccacctc gcctgggctg
     1561 aacacgcgac gctgcccttt gccctgctgc tcatcctgac cggtgtgctg ctgacgctgg
     1621 ggccggagtt cgtctatctg cgggataatt tcggcgtgcg gctcaatacc actttcaagt
     1681 tctattacca ggcctgggcc atgtttggcg tggccgccgt tttcgccctc gactatctgc
     1741 tggccacacg ccagcagggc agtcaacggc tggtgatcgt cgggacgaca gccgtctatg
     1801 tggccctgct ggccgtggcg ctgctattcc cgttctacgc ggtgcagtcg cgggcggccg
     1861 agtaccgcgg cccggcgcgc gatagcgccg gcgcgcgact ggagcggcag ccggccacgc
     1921 tcgacggcct ggcggcgctg caacgggcca atcccagcga gtatgaggcc ctggcctggc
     1981 tgcgcaacaa tgccgtggtg cctggcgaag cgccaccggt ggtgttggag gccgtcggcg
     2041 ggcagtacag cgccttcggc cgcgtcgccg ccaacaccgg tttgccaacc gtgctgggct
     2101 gggcggggca cgaatggcag tggcgcggca gcgaccaccc cgagccgggc cgccgcgggc
     2161 cgctggtcga acagatctac accacgcccg acctgaacgc cgtcgccttc ctgctcgatg
     2221 agctaaatgt gcgctatatc tacgttggcg acctggaaac cagtacctat ggtggggttg
     2281 ggctagagaa gttccgcgac cggctggagg ttgccttcgc caatgaccgt gttacgattt
     2341 accgctggca gccaggaagc ccgaactaga ccatgatgag cgaaggactg gcggaaacac
     2401 aggagacgcg cgtggccgcg ctgccggccg ggccgcggcc cagcgccaat ggccgtccgg
     2461 cggctcccag gacgtggcgg acggccctga ccgtcgccga cggcatcatg ctgctcatcg
     2521 tcggcatcgc cgccgtggcc cgcctggtca atctgggggc gcagccactt tcgccaaccg
     2581 aagccgaggc ggcgttggct ggctggcagt tcgcccacgg cattcccctg acggctgctc
     2641 cggccagccc agcctacttc accctcaccg gcctgctcat gagcctgggc ggcgatggcg
     2701 atacggcggc gcggctggcg ccggcgctct tcggcatcct gacggtcgct ctgccgtggc
     2761 tgtggcgcgg gcgcttccag ccggcggttt ggctgacggc cggcgtcctg ctggccttct
     2821 cgcccatcca gatgatcctc tcgcgcacgg ccggcggcga cgctatcgcc ctgtttgcct
     2881 tgctgctgct ggccgtgacc gcggcgcgcc tggacgaggg cgacggctgg ggcgtggccg
     2941 cgggcgcggc gctggggttg ggcctgacca ccgcgccgct attttacacc gggctggtgg
     3001 cgttcctgcc ggcgtggtgg gtctttcgcg gggccgaaag cgtcgggcgc ggcacgctgc
     3061 acgccggggc actggccgcg gtcatcgcct tcatcaccgt cgccgccggc gggttgttct
     3121 acccggaggg cctcggcggg gcgctgatgc tgctgccgca gtggctgggc cagttcggtc
     3181 tggccggcgg cgcagcggcc cgtgagccgc tgctggtgct gctgcgctat gagttgggtc
     3241 tgttcctgct gggtattccc gcggccgtag gcgcgcttag cggcgacaac aacgtgggca
     3301 aacagttggt gctgtggctg gggctgttgc tgccggtcat cctcctccag atggccctcc
     3361 cccaccaggc cgcggccgtg ctcgtgcccg gctacctgct catcggcctg ctggccggcg
     3421 acctgatgcg cggcgagctg ccgcggacgg agcagcggcg ggcctggctg gtgggcgggg
     3481 gtctggcgct gctgggcatg acgctgctgg tcagcatcgc ccgctttacc cagctggggc
     3541 tatggacggg gagcaatgcg tcgctgatca gcctggcggt gctggctttc gccctggccg
     3601 gcgtggccgt catcgtcgtt ctggcgtggg aaagcccggc ggcgcggcgc ggcgcgttcc
     3661 tggccgtcgt ggccatgttg ctctactggc aatggggcac gggctggcat ctaagccagg
     3721 cggcggccaa cgacccc
//
LOCUS       DAOSMQ010000313         3729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24750_length_3729_cov_4.236799, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000313 DAOSMQ010000000
VERSION     DAOSMQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24750_length_3729_cov_4.236799"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK829_10440"
     CDS             <1..279
                     /locus_tag="PK829_10440"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOU41672.1"
                     /translation="SVEVWQDGDLAGGLYGVSIRGFFAGESMFSRQRDASKVALYYLV
                     QHLRARDFRLLDTQFQTSHLMKMGVVEISRRRYRALLRQALAVKTAFK"
     gene            386..1390
                     /locus_tag="PK829_10445"
     CDS             386..1390
                     /locus_tag="PK829_10445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOU41673.1"
                     /translation="MSLKALPLTQSDVRLFSGRSNPGLAAAIASWLGIRLEETHFSRF
                     SNDNLFVQLGASVRGRAVFLVQSLVPPVSDHLMELLMMIDIAKGAAAREVHAVIPYFS
                     YARSDKKDAPRISITARLVSDLLRTAGATHIMTMTLHSPQVHGFFPVPADPLTARGLF
                     ADYFRPHAFSAEDTVVVAPDVGSAKSAVRFADMLGLGTATAQKTRLSDSKVQIGGLIG
                     RQVSGFRRALIHDDEIATGGTIEELTRLLLDNGLEEIYVICTHGLFLHGALDRLCDIP
                     QICEIVTTDTVPQRSSRDYPKLVALSTGPVFAGAIRQNYYRQSIGDLFDFGDHDDTDQ
                     "
     gene            complement(1428..2696)
                     /locus_tag="PK829_10450"
     CDS             complement(1428..2696)
                     /locus_tag="PK829_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU41674.1"
                     /translation="MKRKWLLFSLLAVLVGAYAARPAAAQDDAPAVVALPVEAGDTWV
                     ALAARYGVSDTTLRALNPHLNRAFQPAIGGTVNLPADASPRTGRLLRAANGGLLEIAA
                     GARLSPWALAAGNALASPYRPTLYSPLLLPGPGTIRDLPPGIETLEVSPWPAAAGQAL
                     GLRGHVAAGVTEVAVRLDNSAGPATVDGGRFLALIGTGAFFAGGRPELVVRVGNGPAW
                     VQPWQFVERQWDYQDLTLTGEAAAAADQQARDEERARLRALWTTITPGPRWDAAFRLP
                     LDDYLSISANYGGRRSYNGGPYLTYHEGVDFSAYGGTPIYAPAAGTVVLAERLYVRGG
                     AVIIDHGLGVFSGFYHMSAVHAAIGQEVQPGDLLGEVGTTGLSTGNHLHWDLLINGVW
                     VDAAVWQEQDLACWALAALARDCAPAAPSP"
     gene            2854..3390
                     /gene="folK"
                     /locus_tag="PK829_10455"
     CDS             2854..3390
                     /gene="folK"
                     /locus_tag="PK829_10455"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU41675.1"
                     /translation="MSLMYLSLGSNLNDRHANLQQAIAQLGEHFTITAISPVYATEPW
                     GDADQPPFLNVCVAAATTTPPHDVLRIVKDIETAMGRQPTRRWGPRLIDIDILFYDKL
                     VLADKELTIPHPRMDERAFVLAPLADIIPTFRHPQTGETVEEMLGRVDVSGVERLFEM
                     QFPNKARDVALSQSQASA"
     gene            complement(3447..3698)
                     /locus_tag="PK829_10460"
     CDS             complement(3447..3698)
                     /locus_tag="PK829_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOU41676.1"
                     /translation="MSAPSPRKRRAWATARATTSSVETWATLSRATLSRATFSRATLS
                     RATFSRATLSRARLSRATFSRATPSPEKLSGVALMMPIT"
     gene            3670..3729
                     /locus_tag="PK829_10465"
                     /pseudo
     CDS             3670..3729
                     /locus_tag="PK829_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          667 a         1193 c         1205 g          664 t
ORIGIN      
        1 agcgtcgagg tctggcagga cggcgatctc gccggcggcc tctatggcgt cagcatccgc
       61 ggctttttcg ccggcgaaag catgtttagc cggcagcggg acgccagcaa ggtagcgctg
      121 tactatctcg tccagcatct gcgggcgcgc gacttccgcc tgttggacac gcagttccag
      181 acgtctcacc tgatgaagat gggcgtggta gaaatctcgc gccgccgcta ccgtgctctg
      241 ctgcggcaag cgctggccgt gaaaactgcg tttaaatagg gctgactagt ttgatcgcgc
      301 gccatcggcg ggcataatat gtgcaagcat ctgcgcgcca gagcgcggcg gtcgcgctgg
      361 cccgcgttca tcccaggagg tagccatgag cctcaaagct ctccccctca cgcagagtga
      421 tgtccgtcta tttagtggtc gctcgaatcc gggtttagcc gcggcgattg ccagctggct
      481 ggggatacgg ctggaggaaa cgcacttctc gcgtttcagc aatgacaacc tgtttgtgca
      541 gttgggcgcc agtgtgcgcg gccgggccgt ctttctggtg cagtcgcttg tgccgccggt
      601 cagcgatcat ctgatggaat tgctcatgat gatcgacatc gccaaaggcg cggcggcgcg
      661 ggaagttcat gcggtcatcc cctacttctc ctatgcccgc tccgacaaaa aggacgcgcc
      721 gcgcatttcc atcacggccc gcctcgtgtc cgacctgctg cgcactgccg gggcgaccca
      781 catcatgacc atgaccctgc actcgcccca ggtgcacggt ttcttccctg tgcctgccga
      841 cccgttgacc gcgcgcgggt tgtttgctga ctatttccgc ccgcacgcgt ttagcgcgga
      901 ggacaccgtt gtcgttgcgc ccgatgtagg cagcgccaaa tcagcggtgc gcttcgccga
      961 catgctgggt ctgggaacgg cgacggccca aaagacgcgc ctgtccgact ccaaagtgca
     1021 gatcggtggg ctgatcggcc ggcaagtcag cggtttccgg cgcgcgctaa tccacgacga
     1081 cgagatcgcc accggcggca caatcgagga actgacccgc ctgctgctgg acaacggcct
     1141 ggaagagatc tacgtcatct gcactcacgg cctgttcctc cacggcgcgc tggatcggct
     1201 ttgtgacatt cctcagattt gcgaaatcgt cacgaccgac accgtgccgc aacgctcgtc
     1261 gcgggattat cccaagctgg tcgctctgtc aactggtccc gtgttcgccg gagccattcg
     1321 ccagaactac tatcgccagt ccattggcga tctatttgac ttcggcgacc acgacgatac
     1381 tgatcagtag gctgctgtcc gcgcctttgg gagcagtaga gtaaccccta tggactgggc
     1441 gcggccggcg cgcagtcgcg cgccagggcc gccaaagccc agcaggccag gtcctgttct
     1501 tgccacacgg ccgcatcgac ccagacgccg ttgatgagca gatcccagtg cagatgattg
     1561 cccgtggaca ggcccgtggt gcccacttcg cccagcaagt cgccgggttg cacctcctgg
     1621 ccgatggcgg cgtggacggc cgacatgtgg taaaagccgg agaaaacgcc caggccgtgg
     1681 tcgataatga ccgcgccgcc gcgcacgtaa agccgctcgg ccaagacgac tgtacccgcc
     1741 gccggagcgt agatgggcgt gccaccgtaa gcgctgaagt cgacgccctc gtggtaggtc
     1801 agataggggc cgccgttgta ggagcggcgg ccgccgtagt tggccgaaat gctcagatag
     1861 tcgtcgagcg gcaagcggaa ggcggcgtcc cagcgcgggc ccggcgtgat cgttgtccat
     1921 agggcgcgca ggcgcgcgcg ttcctcgtcg cgcgcctgtt gatcggcggc ggcggccgct
     1981 tcgccggtca gggtcaagtc ctggtagtcc cactgccgct cgacgaactg ccacggctgg
     2041 acccaggccg ggccgttgcc gacgcgtaca acaagctctg gccggccacc ggcgaaaaat
     2101 gcccccgtac caatcagagc taggaagcgc ccgccatcaa ccgtcgccgg gccggccgag
     2161 ttgtctagcc ggacggcaac ttcggtcact ccagcggcga cgtggccacg caggccgagc
     2221 gcctggccgg cggctgccgg ccagggcgac acctccagcg tttcaattcc gggcggcagg
     2281 tcgcggatcg tacccgggcc aggcaacaat agggggctat agagggtggg ccggtaaggg
     2341 gaagccagcg cgttgccggc cgccagcgcc cacggcgaca gacgcgcgcc cgcggcgatt
     2401 tccagtagcc caccgttagc tgctcgcagc aggcggccgg ttcgcgggct tgcgtccgcg
     2461 ggcaggttca ccgtgccgcc gatggcgggc tggaaggcgc ggttgagatg cgggttcagg
     2521 gcgcgcaacg tcgtatcgct tacgccatag cgcgcggcca gcgccaccca ggtgtccccc
     2581 gcttcgacag gcagggccac gacggccggc gcgtcgtctt gcgccgccgc tggccgcgcc
     2641 gcatacgccc ccaccaagac tgccagtagc gagaaaagga gccactttcg cttcacaggg
     2701 ctattctaac cgtcgagcgc cgcgaagcca cccaagtccc gccggtcaat tcgttgacac
     2761 tcatgacgca gtgtgttata tatgtgtata ataatgcagt gcgtcatatc ggttatcctt
     2821 aacaggcggg acacattgag gcggtgaaca gttatgagtc tgatgtactt aagtcttggg
     2881 tcgaacctga acgatcggca tgccaacctc caacaggcta tcgcccaatt gggggagcat
     2941 ttcaccatca cggccatttc accggtctac gcaaccgaac cgtggggcga tgccgaccag
     3001 ccgccgttcc taaacgtgtg cgttgccgcc gccacgacca cgccaccgca tgacgtcctt
     3061 cgcatcgtta aggatattga gacagcgatg gggcgccagc cgacgcgccg ctggggaccg
     3121 cggctcattg acatcgacat ccttttctat gacaagctcg ttctggctga caaggaattg
     3181 acgatcccgc atccacgcat ggatgagcgc gccttcgtac tggcgccgct ggccgacatc
     3241 attcccacct tccgtcatcc acagacaggc gaaacggtcg aagagatgct cggccgggtc
     3301 gatgtgtcgg gcgtggagcg gctatttgag atgcagtttc ccaacaaggc gcgtgacgtt
     3361 gccctgagcc agagccaggc ctcggcctga catcggccgg ccacgtaggc gctcggaacc
     3421 atgatgttcg agtggggtca acgcacctac gtaatgggca tcatcaatgc cacgccggac
     3481 agcttttccg gcgatggcgt ggcacgtgag aacgtggcac gtgagagcct ggcacgtgag
     3541 agcgtggccc gtgagaacgt ggcccgtgag agcgtggccc gtgagaacgt ggcccgtgag
     3601 agcgtggcac gtgagagcgt ggcccaggtt tcaaccgaag aggtggtcgc gcgggcggtg
     3661 gcccaggcgc ggcgctttct gggcgatgga gccgacattc tggacgtcgg cggcgagagc
     3721 acgcggccg
//
LOCUS       DAOSMQ010000314         3727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24768_length_3727_cov_4.049292, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000314 DAOSMQ010000000
VERSION     DAOSMQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24768_length_3727_cov_4.049292"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3165
                     /locus_tag="PK829_10470"
     CDS             <1..3165
                     /locus_tag="PK829_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOU41677.1"
                     /translation="DIRASFNELWNGEDRDWLAIDLPDAVRDELIKRAPAHRPERDPL
                     ERPAPGAVRDGEGRPRLAVVTPIDRVLFQFVRDAPTLLNAGDLAAETSAVRPWPHQTQ
                     VARTLIGRFPDRALLCDEVGLGKTIEAGLIIRHLVLSGRVRRALILTPKAVLRQWQEE
                     LYEKFNLAIPRYEDGRLIGVYETSGPPIARPWDESDLLLATSHLARRRERVEELAAAP
                     RWDLLVVDEAHHARRKDFLQPTYRPNRLLTLLNRLKAADRYDGLLLMTATPMQVHPVE
                     VWDLLTVLGLSGRWGADERAFLDFFAELRRLPADTDWDLVYSLVNDYLSHVSLDGQID
                     PDFVRAMASELGAPRAEIIRTLPENLAPGRVVRALPPAAQQYAAELARRHTPLQRYLF
                     RNTRTLLRRYVDKGLLKANVPTRRPQIRRVAFRADEAELYGRITEYISQFYARYESER
                     RGLGFIMTVYRRRLTSSFYAVRRSLERRRDFLLRQIPASEAFTADDVTELDEEDALEQ
                     AGLDFEDLNAVDMTSPGMRAAFDAELTYLETFINDLRRLSGNDSKVDALKNELDRVFR
                     QRPTALVFTQYTDTMDYLRDHLVTVYGSKIACYSGRGGEVWNGLTWVQTSKEWVKEQF
                     HDGEIAILLCTESASEGLNLQTCGMLANYDMPWNPMRVEQRIGRIDRIGQTYPEVWIS
                     NYFYEDSIEDKVYRALEDRIQWFEDVVGALQPILAEVGEVTRKLAMLPAEEQAARFES
                     EMRELRRKLEESKINALDIDRYVELDEPDPTVASPVRLADLEALLTTSAATGHLFTGH
                     ETIAGAWWLQWDRQQMAVTFRAELFDRWPQTLQFLSYGSPLLEAVLALVEPPDELPAG
                     VARYEAADLPLRGWYDLSAGAPRPVGSLAELQRLAAAGQPAHAGDPEARAATAQAAFA
                     AEAAARRDLNAQRRARLAGERQIVLAEQARQLVTQAALVEIALGRQKSLFDDEIYPSS
                     FNEAAVGGLRRHKSPWSWIIYLLGQTGVPLPLPREDDPFFQQIAGEGVERLKDRFVRL
                     TSETREVVAEWRAVQFSSHTPAAR"
     gene            3419..>3727
                     /locus_tag="PK829_10475"
     CDS             3419..>3727
                     /locus_tag="PK829_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU41678.1"
                     /translation="MYERVLVIDDSQDIRDFLIENILRPKGFEVLAASDGLMGLELAI
                     AKEPDLMITDQQMPGLTGLQVLQKLRERNIDIPAILVTGEGSEDTAVRAFRLGIKDYI
                     I"
BASE COUNT          635 a         1266 c         1235 g          591 t
ORIGIN      
        1 gacatccgcg cctccttcaa cgaactgtgg aacggcgaag accgcgactg gctggccatc
       61 gacctgcccg acgccgtccg cgacgaactc atcaagcgcg ccccagccca ccgccccgaa
      121 cgcgacccgc tggaacgccc ggcgcccggc gccgtccgcg atggcgaagg ccggccgagg
      181 ttggccgtcg tcacgcccat tgaccgcgtg ctcttccagt tcgtgcgcga cgcccccacc
      241 ctgctcaacg ccggcgacct ggcggccgag acgagcgccg tccgcccctg gccccatcag
      301 acgcaggtgg cccgcaccct catcggccgc ttccccgacc gggcgctgct gtgcgacgag
      361 gttgggctgg gcaagaccat cgaggccggg ctgatcatcc gccacctggt gctcagcggc
      421 cgcgtgcggc gcgccctcat cctgaccccc aaggccgtgc tgcgccagtg gcaggaagag
      481 ttatacgaaa agttcaacct ggccattccc cgctacgaag acggccgtct gatcggcgtc
      541 tacgaaacga gcggcccgcc tatcgcccgg ccgtgggacg agagcgacct gctactagct
      601 accagccacc tggcccgccg tcgcgagcgg gtggaggagc tggccgccgc gccgcgctgg
      661 gacttgctgg tcgtcgacga ggcccaccac gcccgccgca aggactttct ccagccgacg
      721 taccgcccca accggctgct gacgctgctg aatcggctga aggccgccga ccgttacgac
      781 ggcctgctgc tcatgacggc cacgccgatg caggtgcacc cggtcgaagt gtgggatctg
      841 ttgaccgtgc tgggcctcag cggccggtgg ggggccgacg agcgcgcctt cctcgacttc
      901 ttcgccgagt tgcgccgcct gccggccgac acggactggg acctggtcta cagcctggtc
      961 aacgactacc tgagccacgt ctcgcttgac gggcagatcg accctgactt cgtgcgcgcg
     1021 atggccagtg agctgggcgc gccgcgggca gagatcatcc gcaccttgcc ggaaaacctc
     1081 gctccgggcc gggtggtgcg cgccttaccg ccggccgccc agcaatacgc cgccgaactg
     1141 gcccgccgcc acaccccgct gcagcgctac ctcttccgca acacgcgcac gctgctgcgg
     1201 cgctacgtgg acaaagggct gctgaaggcc aacgttccca cccgccggcc gcagatccgc
     1261 cgggtggcct tccgcgccga cgaggccgaa ctctacgggc gcatcaccga gtacatcagc
     1321 cagttctacg cccgctacga gagcgagcgg cgcgggctgg gcttcatcat gaccgtctac
     1381 cgccgccggc tgacgagcag cttctacgcc gtccggcgca gcctggagcg ccggcgcgac
     1441 ttcctgctgc gccagattcc ggccagcgag gccttcaccg ctgacgatgt gaccgaactg
     1501 gacgaggagg acgcgctgga acaggccggg ttggactttg aggaccttaa tgcagtggat
     1561 atgacctcgc ccggcatgcg cgccgctttt gacgccgagc tgacctacct ggaaacgttc
     1621 atcaatgacc tgcggcgact cagtggcaac gactccaagg tggacgcgct gaagaacgag
     1681 cttgatcggg tcttccgcca gcggccgacg gcgctcgtct tcacccagta taccgacacg
     1741 atggactatc tgcgcgacca tttggtgacc gtctacggct caaaaatcgc ttgctactcc
     1801 gggcgtggcg gcgaggtgtg gaacggtctg acctgggtgc agacgtccaa ggagtgggtc
     1861 aaggagcagt tccacgacgg cgagatcgcc atcctgctct gcaccgagtc ggccagcgag
     1921 ggcctgaacc tgcaaacgtg cggcatgctg gccaattacg atatgccctg gaaccccatg
     1981 cgcgtcgagc agcgcatcgg ccgcatcgac cgcattgggc agacctatcc cgaggtctgg
     2041 atcagcaact acttctacga agacagcatc gaggacaagg tctaccgggc gctggaggat
     2101 cgcatccaat ggttcgagga cgtcgtcggc gcgttgcaac cgatcctggc cgaggtcggc
     2161 gaggtgacgc gcaagctggc catgctgccg gccgaggagc aggcggcccg tttcgagagc
     2221 gagatgcgcg agctgcggcg caagctggag gagtcgaaga tcaacgccct cgacatcgac
     2281 cgctacgtcg aactggacga gccggatccc acggtagcct cgcccgtgcg gctggccgac
     2341 ctggaagcgc tgctgaccac gtccgcggcg acgggccacc tgttcacggg ccacgagacg
     2401 attgccggcg cgtggtggct gcaatgggac aggcagcaga tggccgtcac cttccgcgcc
     2461 gagttgttcg accgctggcc gcagacgctc cagttcctat cctacggcag tccgctgctg
     2521 gaggcggtgc tggcgctcgt ggaaccgccg gatgaactgc cggccggtgt ggcgcgctat
     2581 gaggcggctg acctgccgct gcggggctgg tatgatctga gcgccggcgc gccgcggccg
     2641 gtcgggtcgc tggccgagct acaacggctg gcagccgccg gtcaaccggc gcacgccggc
     2701 gacccagagg ccagggccgc gacggcgcag gcggctttcg cggccgaagc agccgcccgt
     2761 cgggacttga acgcgcaacg ccgggcgcgg ctggccggcg aacggcagat tgtcctggcc
     2821 gagcaggccc ggcaactggt cacgcaggcg gcgctggtgg agatcgccct tggccgccag
     2881 aagtcgctct tcgacgacga gatctatccg agcagcttca atgaggcggc tgtcggcggt
     2941 ttgcgacggc acaagagtcc ttggtcgtgg atcatttatc tactgggaca gacaggagtg
     3001 cccctgccgc tgccccggga ggacgatccg ttcttccagc agatcgccgg cgagggagtg
     3061 gaacggctga aagatcgctt cgttcggctg acgtcggaga cgcgggaggt ggtggcggag
     3121 tggcgcgcag ttcagttctc cagccatacc cctgctgcgc ggtaattaaa gtccggcggc
     3181 caccttgtgc tcagccagtg ggcggtgccc tctgttgatg gggcctcggt gccggcccac
     3241 caacacgctg ctggggccaa aaaagccacc atgtgtcccg acctgacagg tgggcggccg
     3301 ttcacctgtc aggtcggtgc gcccggggtg acattggcaa agccctgtgg tcagttgcct
     3361 gcccatgggg cgagtagtac actttctgaa actgttccgt tccctggcag agtggcctat
     3421 gtacgaacga gtactggtga ttgacgattc gcaggacatc cgcgacttcc tgatcgagaa
     3481 catcctgcgg ccgaagggct tcgaggtgct ggccgcgtcc gatggtctca tgggcctgga
     3541 actggccatc gccaaagagc cagacctgat gatcactgac cagcagatgc cgggactgac
     3601 cggcctgcaa gtcctgcaga agttacgcga gcggaacatc gacatccccg ccatcctcgt
     3661 caccggcgag ggttcggagg acacggccgt gcgcgccttc cgcctgggca tcaaggacta
     3721 catcatc
//
LOCUS       DAOSMQ010000315         3705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_24941_length_3705_cov_5.298630, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000315 DAOSMQ010000000
VERSION     DAOSMQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24941_length_3705_cov_5.298630"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..1910)
                     /locus_tag="PK829_10480"
     CDS             complement(372..1910)
                     /locus_tag="PK829_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HOU41679.1"
                     /translation="MAIPSLLLKQLHTFGSLKNTADGVEFSVKNRLSDTTLTGLTGLK
                     FDGYEVPLDSILVRLPDGTTLTPAQLAAAPLDFPLRRTLDIVAAIDPLPIGKHEIEIR
                     FEAQPFGRLKLKVDDAITADRPKTVHIPRDNEDDYSDKAIKARQQFIEDVSGAKLEHT
                     ARYSFDPHMVAGNAEHFTGVAQIPLGFAGPITIRGEHAQGDFFIPLATTEGTLIASYN
                     RGMKILNLSGGVTCTVIGDAMQRAPVFVFKTAREARHFVSWVEANIGEVRDQAEATSS
                     VAKLQYIDYYLAAKLAYLRFNFSTGDAAGQNMVGRATFAACSWILDHYDGVQHFYLES
                     NLATDKKASQVNIMHTRGKRVVAEATIKRDVLLQHMRVDPENLYYHAGIANVGAILSG
                     ANNNGLHSANGITAMFIATGQDVANVSESSAGILYTELTPEKDLYLSITIPSLIVATY
                     GGGTGLATQRECLSILGCVGKGKVNKLAEIIAGVVLAGEISLAAAISSSDWVSSHEKY
                     GRNR"
     gene            complement(2011..>3705)
                     /locus_tag="PK829_10485"
     CDS             complement(2011..>3705)
                     /locus_tag="PK829_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41680.1"
                     /translation="LASSFLHDFMWNVAGNTGSDLDAFAEGAPGFGFIYLPLETVAGY
                     HNQADNLAALDPRSVQGMGDLALTLTRAFGNRPLDELTGAPDRVAFPLARGVLVDYSS
                     SLSLPLAIVATALLAAALVLGLRRGRLTGRGLLAAFALGAPLMLTAVLVVTGIWWLVR
                     LLTPGLHTFTVGGWWGNGFYLTASLALALAVALAWRGMLRFRADDLLAGGLLWFALLA
                     LVTALLLPGIGYLFVWPLLVVALGWVVSALRPGGKWAALPVVVGAFVALVLAAPVAYW
                     LWVYVGRAEAQMGIPFAALPVLFVLPALLLAAAAVEALRSTATAPVAQRRVALGLVRR
                     PWFAFLALALLLFAIPALRRPAPARPWVNTVVYTLDAGEGAAYWVTFNDSRMGRGTRQ
                     QLDEWTRQFFPAGAEATTFDPWLVTRSDTLYPALRAPAPVVALPHTTITATYTGGEAQ
                     LTLSRPPEAMLTRLVVHTTAPITAVTLDGRPLDLAGTQPAEYTVLVLGRADTLTLALG
                     GLSQGDATIEVLDRLTTDVMTIAAQAGLMINPRPAWMGVASASDTGEGALVTTRFE"
BASE COUNT          683 a         1194 c         1245 g          583 t
ORIGIN      
        1 tttttttttt tcaagcagaa gacggcatac gagataggag tgaatgaggc ccatccggtt
       61 ggcctcatcc tggttggtca agttgtccgc ctgcctgacc ggcgtgaggc aactaatctt
      121 gtagcaggag aagcacaatg cctaagagtg gtgacaagaa gaaagggacc gacaagaaca
      181 agccgccgcg gaccataaag gagaagcagg aggcgaagaa ggaaaagaag aagaagaagg
      241 aaacaggcta acaacggccg cgcgacttat gaaacgggtg gagccgtgag gcggctccac
      301 ccgttatctt ttggcccttt tcgtccttgg cccaagcctc ttacttcgtc gcgcgtaaac
      361 cgtcgctccc tctaccgatt ccgcccatac ttctcatggc tgctcaccca gtctgacgac
      421 gagatggccg cggccaggga gatttcgccg gccagcacga cgccggcgat gatctcggcc
      481 agcttgttga ccttgccctt gcccacgcag ccgaggatgc tcaggcattc gcgctgcgtg
      541 gccaggcccg tgccaccgcc ataggtggcc acgatgagcg acgggatggt gatggacaga
      601 tagaggtctt tctccggcgt tagctcggtg tagaggatgc cggccgatga ctcggacaca
      661 ttggccacgt cctggccggt ggcgatgaac atggccgtga tgccgttggc cgagtgcagc
      721 ccgttgttgt tggccccgct gaggatcgcg ccgacgttgg cgatgccggc gtggtagtaa
      781 aggttctccg gatcgacgcg catgtgctgc aagagcacat cgcgcttgat cgtcgcctcg
      841 gccacgaccc gcttgccgcg cgtgtgcatg atgttgacct gcgacgcctt cttgtccgtg
      901 gccaggttcg actccagata gaagtgctgg acgccgtcgt agtggtcgag aatccagctg
      961 cacgcggcga aggtggcccg gccgaccatg ttctgcccgg cggcgtcgcc cgtgctgaag
     1021 ttgaagcgca gataggccag cttggcggcc aggtagtagt cgatgtactg caacttggcc
     1081 acgctggagg tcgcctcggc ctggtcgcgc acctcgccga tgttggcctc gacccagctg
     1141 acgaagtggc gcgcctcgcg ggccgtcttg aagacaaaca ccggcgcgcg ctgcatggcg
     1201 tcgccgatga ccgtgcaggt taccccgccc gacagattga ggatcttcat gccccggttg
     1261 tagctggcga tgagcgtgcc ctccgtcgtc gccaggggga tgaagaagtc gccctgggcg
     1321 tgctcgcccc tgatggtgat tggcccggca aaacccagcg gaatctgggc cacgccggtg
     1381 aagtgctcgg cgttgccggc gaccatgtgg gggtcgaagg agtagcgagc ggtgtgctcc
     1441 agcttggccc cgctcacgtc ttcgatgaac tgttggcggg ccttgatggc cttgtcgctg
     1501 tagtcgtcct cgttgtcgcg ggggatgtgg acggtcttgg gccggtcggc ggtgatggca
     1561 tcgtccacct tcagcttgag ccggccgaac ggctgcgcct cgaagcgtat ctcgatctcg
     1621 tgcttaccga tgggcagcgg gtcgatggcg gcgacaatgt cgagcgtgcg gcgcaagggg
     1681 aagtccagcg gcgcggcggc cagttgcgcc ggggtcagcg tcgtcccgtc tggcagccgg
     1741 acgaggatcg agtcgagcgg cacctcgtag ccgtcgaact tcaggccggt caggccggtc
     1801 agcgtcgtgt cgctaagccg gttcttgacg gagaattcga cgccgtcggc ggtgttcttc
     1861 agactgccga aagtgtgcaa ctgcttcaac aacaggctgg ggatcgccaa tgccatgggt
     1921 gtcctccttg agtgctgggt agataatgtc cgtgtggcgg gaattatacc cgcctctcag
     1981 gagggcgaaa ggaaatggcc gccctctctg tcactcaaac cgcgtggtca caagcgcgcc
     2041 ctcgcccgtg tccgatgccg atgccacacc catccacgcc ggccgcggat tgatcatcag
     2101 gcccgcctgc gcggcaatcg tcatcacgtc ggtcgtcagc cggtcgagaa cttcgatggt
     2161 cgcgtccccc tggctcagcc cgcccagcgc cagcgtcagc gtgtcggcgc gaccgagcac
     2221 gaggacggtg tactcggccg gctgtgtgcc agccaggtcg agcggccggc cgtccagcgt
     2281 cacggcggtg attggcgcag tggtatgcac taccagccgc gtcagcatcg cctccggcgg
     2341 ccggctgagg gttagctggg cctctccgcc ggtgtaggtg gcggtgatgg tcgtatgcgg
     2401 cagggccacg actggcgcgg gcgcgcgcaa agccgggtag agcgtgtcgc tgcgcgtcac
     2461 caaccaggga tcgaacgtcg tcgcctcggc cccagcgggg aagaattggc gcgtccactc
     2521 gtctagttgc tgccgcgtac ctcgacccat gcgggaatcg ttgaaggtca cccagtaggc
     2581 cgcgccctcg ccggcgtcga gcgtgtagac gacggtattg acccacggcc gtgccggcgc
     2641 cggccggcgc agtgccggga tggcgaagag caacagggcc agcgccagga aggcgaacca
     2701 cgggcggcgc acaagcccca gggcgacgcg ccgttgcgcc accggcgcag tggcggttga
     2761 acgcaacgcc tccacagccg cggccgccag caacagggct ggcaacacaa acagcaccgg
     2821 cagggcggcg aaggggatgc ccatctgcgc ctcggcccgg ccgacgtaga cccacagcca
     2881 gtaggccact ggcgcggcca gcaccagagc cacgaacgcc ccgacaacca ccggcagcgc
     2941 cgcccacttc ccgcccggcc gcagggccga caccacccag cccaacgcca cgaccagcaa
     3001 cggccagacg aacagatagc cgatgccggg caggagcaga gcggtgacga gagccagcag
     3061 ggcaaaccag agcaacccgc cggccaacag atcgtcggcc cggaagcgca gcatcccacg
     3121 ccaggcgagg gccacggcca acgccagggc cagcgacgcg gtcagataga agccgttacc
     3181 ccaccaaccg ccgacagtga aggtgtgcag ccccggcgtc agtagcctca ccagccacca
     3241 gatgccggtg acgaccaaaa cggccgtcag catgagcggc gcgcccaagg cgaaagcagc
     3301 cagcagcccc ctgcccgtca gccggccgcg ccgcagcccc agcaccaatg ccgccgccag
     3361 cagcgccgtc gcgacaatgg ccagcggcag agacaggctg cttgaatagt caactaacac
     3421 gccccgcgcc agcggaaagg ccacccggtc aggcgcgccg gttagctcgt ctagcgggcg
     3481 gttcccgaag gcgcgcgtca gcgtcagcgc gagatcgccc atgccctgca cgctgcgtgg
     3541 atccagcgcg gcgaggttgt cggcctggtt atggtagccg gccaccgtct ccagtggcag
     3601 gtagatgaag ccgaagccgg gcgcgccctc agcgaaggcg tccaggtcag agccggtatt
     3661 gccggcaacg ttccacatga agtcatggag gaaggaactg gccag
//
LOCUS       DAOSMQ010000316         3698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25017_length_3698_cov_3.907494, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000316 DAOSMQ010000000
VERSION     DAOSMQ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25017_length_3698_cov_3.907494"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /gene="rsmG"
                     /locus_tag="PK829_10490"
     CDS             <1..596
                     /gene="rsmG"
                     /locus_tag="PK829_10490"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOU41681.1"
                     /translation="LTAIREPRQIRIRHFLDALSCAVATGPLDGRRLIDVGSGAGFPG
                     LPLKILYPGLRLTLVDSVAKKARFLETVASELGLGDVVVIADRAEALGQAAGQREAYD
                     WAAARAVAELRVLAELLLPLCRVGGRMLAQKGDSAAAELAAAGPAIGAVGGGVARLTP
                     VHLPETEAVHYLVIIDKARPTAARYPRRVGVPAKKPL"
     gene            complement(700..1947)
                     /gene="recF"
                     /locus_tag="PK829_10495"
     CDS             complement(700..1947)
                     /gene="recF"
                     /locus_tag="PK829_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOU41682.1"
                     /translation="MQVRRLSLTNFRNYARLELDLPAGVVLLHGDNAQGKTNLLEAIY
                     FLATTRSPHASIDQQLLNWDALQGDDPVVAGRIVADVLRPDGQRHLEMRLIIEERGAW
                     TSNGQGTFRREALINRRKVRLMDLLGQLRVVLFLPEDVELATGPPANRRRYLNITLCQ
                     VDAEYCRELSNYNKVLEQRNALLRRMAEGQTRNAADVLQVLTDKLVEPASLVLQRRAV
                     FLAEMARLAQDIYFRDLIGGRESLRLGYLPGWYTNGRKAGEELLSAGDQLQETPDRAA
                     IAARFASELALARATDMARGMSTVGPHRDDWAILVNGKNLGPFGSRGQVRTAILALKL
                     AEIGWMKSATDDTPILLLDEVSAELDQHRRAALLAYVLEQARGQGTGQALLTSTDPSL
                     FTAEFLSVAATLRVAGGRVEPGQ"
     gene            2200..3081
                     /locus_tag="PK829_10500"
     CDS             2200..3081
                     /locus_tag="PK829_10500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOU41683.1"
                     /translation="MEEDDASIGSPLRVVLTFLLIVVLPLAIGLFAAARLTPRPQIGI
                     IRLSYDINSQTAYEIGEQLRTARQNPAIKGVVLVINSPGGSAAFSEELFLDVLDTRRE
                     LPVVASIDLLAASGAYYMAAAANEIYAKPTSSVGSVGVIASLPGDIFIDEDLLTTGPY
                     KAFGGTRDGYVRQIERAKLAFLDAVQVGRGDRLTMDLATLSRAEIYTGVQAAEFGLID
                     GLASGEEALRRAAALAGVRQYDAVELYPLTFDTDTIPLPEVRYQPQPIDEARLRQAPA
                     ALAPGLYYRYVEPSAAR"
     gene            3103..>3698
                     /locus_tag="PK829_10505"
     CDS             3103..>3698
                     /locus_tag="PK829_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41684.1"
                     /translation="MRKAALYLAIFVVLLLAPSAVRLFQYYRPLAEAPATPPAYTGAG
                     IGAVPTPEAGNGADTVQTGAATRRVAGAPRGVVVLDQAHGNQFTREELATLDGLLAAR
                     GLQVRPLAEGALTEALRAATGLVVIAPVDGYSPAEAQAVSDLVARGGRVLLVGDPTRY
                     NIEFDETDIFAAPIIATAQAPLNDLANAFDITFRGDYL"
BASE COUNT          582 a         1281 c         1242 g          593 t
ORIGIN      
        1 ccctgacggc cattcgcgag ccgcgccaga ttcgcatccg ccacttcctc gacgcgctca
       61 gttgcgccgt cgcgaccggc ccgctcgatg gccgccgcct gatcgacgtc ggcagcggcg
      121 caggctttcc cggtctgccg ctcaaaatcc tctaccccgg tttgcgcctg acactggttg
      181 acagcgtggc caagaaggcg cgctttctgg agaccgtcgc ctccgagcta ggcctgggcg
      241 atgtcgtcgt catcgccgac cgcgccgaag cgctgggcca ggcggccggc cagcgcgagg
      301 cttacgactg ggccgcggcg cgggcggtgg ccgagttgcg cgtgctggcc gagctactgc
      361 tgcccctctg ccgcgtcggc ggacgaatgc tggcccagaa gggcgatagc gccgcggccg
      421 agttggccgc cgccggcccg gccattggcg ctgttggcgg cggcgtggcg cgcctgacgc
      481 ccgttcactt gccggaaacc gaggccgtcc actacctggt catcatcgac aaggcccggc
      541 cgacggcggc gcgctacccg cgccgggttg gcgtgccggc caaaaagccc ctatagagaa
      601 tggcctgccg ctagtgcgga ttcgaccgat tgacgcgact aagaatgcaa gatggttctt
      661 ggctgcgtct atcggcgtcc gccatgacct atctcagaat tactggccgg gttcgacgcg
      721 gccgccggcg acgcgcaggg tggcggcaac ggacaggaat tcggcggtga agaggctggg
      781 gtcggtggac gtgaggaggg cctggcccgt gccctggccg cgcgcctgtt ccaggacgta
      841 ggccagcagc gccgcccgcc ggtgctggtc gagttcggcg ctaacctcgt cgagcagcag
      901 gatgggcgtg tcgtccgtgg ccgacttcat ccagccgatt tcggccaact tgagggccag
      961 gatggcggtg cgcacctgcc cccgcgagcc gaacggcccc aggttcttgc cgttgaccag
     1021 aatggcccag tcgtcgcggt gggggccgac ggtcgacatg ccgcgggcca tatccgtcgc
     1081 ccgcgccagg gctaactcgg aggcaaagcg cgcggcaatg gccgcccggt cgggcgtttc
     1141 ctggagctgg tcgcccgcgc tcagtagctc ctcgcccgcc ttgcggccgt tggtgtacca
     1201 accgggtaga tagcccagcc gcaaggactc acggccgccg atcaggtcgc ggaagtaaat
     1261 atcctgggcc aggcgcgcca tttcggccaa aaagacggcc cgccgctgca agacgaggct
     1321 ggccggctcc accagcttgt cggtcaatac ttgcagcacg tcggcggcgt tgcgcgtctg
     1381 cccctcggcc atgcggcgca gcagggcatt gcgctgctcc aacaccttgt tgtaattgga
     1441 tagctcgcgg caatactcgg cgtctacctg gcagagggtg atgttgaggt aacgacgccg
     1501 gttggccggc ggcccggtgg ccaattcgac atcctctggc aggaagagaa caacgcgcaa
     1561 ctggccgagc aggtccatca ggcgcacctt gcgccggttg ataagcgcct cgcgccggaa
     1621 ggttccctgg ccgttgctcg tccacgcgcc acgctcctcg atgatcagcc tcatctccag
     1681 gtggcgctgg ccgtctggcc gcagcacatc ggcgacgatg cggccggcca ccacggggtc
     1741 gtcgccctgc agcgcgtccc agttgagcaa ctgttggtcg atgctggcgt ggggcgagcg
     1801 cgtcgtcgcc aggaagtaaa tcgcctccag caggttggtc ttgccctggg cgttgtcgcc
     1861 atgcaacaag acgacgcccg ccgggaggtc aagctccaga cgagcgtagt tgcggaagtt
     1921 ggtcaacgat agacgacgga cctgcatgga tgcaaagata tggcgaaacg gatcgattgg
     1981 caagggccat agggacgcgc ccggcggcat gtgcgccaag ccaggccaga gggcagacgc
     2041 gggggcagag atgtagatac ccagaggcgc aaacacccgg agggcagata cgtagcgtcg
     2101 gcacctgccg ggcgagggag agagggtttg cggcgctctg gcccgcgtgc tatgctcacg
     2161 ttgctacggt cggccgcggt cgccgcagca ggagattcga tggaagaaga cgacgcctcg
     2221 atcggctcgc ccttgcgcgt tgtgctgaca tttctgctca tcgtcgtgct gccgctggcg
     2281 attggcctgt tcgcggccgc gcgcctaacg cccaggcccc agatcggcat catccgcctt
     2341 agctacgaca ttaacagcca gaccgcctat gagatcggcg agcaactgcg gacggcgcgc
     2401 cagaacccgg ccatcaaggg tgtcgtcctg gtcatcaaca gccccggcgg ctcggcagcc
     2461 ttcagcgagg aactcttcct cgacgtgctg gacacgcgcc gcgagttgcc cgtcgtcgcc
     2521 tctatcgacc tgctggcggc cagcggcgcg tactacatgg ccgcggcggc caacgagatc
     2581 tatgccaagc ccacctcttc cgtcggcagc gtcggtgtca tcgcctccct gccgggcgac
     2641 attttcatcg acgaagacct gctgacgacc ggcccctata aggccttcgg cggcacgcgc
     2701 gacggctacg tgcgccagat cgagcgggcc aagctggctt tcctcgatgc cgtgcaggtc
     2761 ggccgcggcg accgcctgac gatggatctg gcgacgctgt cgcgggccga gatctacact
     2821 ggtgtgcaag cggccgaatt cggtctcatc gacggcctgg cctccggtga ggaagcgctg
     2881 cggcgggctg cggccctggc cggcgtgcgc cagtacgacg ccgtcgagct atacccgctg
     2941 accttcgaca cagacaccat ccctctgcca gaagtgcgct accagccgca gccaattgac
     3001 gaggcccgcc tgcgacaggc tccggccgcg ctggcccccg gcctctatta ccgctacgtc
     3061 gagccgagcg cggctcgctg accgccacac ccccggaata ccatgcgtaa agccgccctc
     3121 tatctggcta tcttcgttgt cttgctgttg gcgccgtcgg ccgtgcgcct tttccagtac
     3181 taccgcccgc tggccgaggc cccggcgacc ccgccggcct acaccggcgc gggcattggc
     3241 gctgtgccca cacccgaagc gggcaacggc gcggacaccg ttcagaccgg cgcagcgacc
     3301 cggcgcgttg ccggcgcgcc gcgcggcgtc gtcgttctcg accaggccca cggcaaccag
     3361 ttcacgcgcg aagagttggc cacgttggac gggctgctgg cggcgcgcgg cctgcaagtg
     3421 cggccgctgg ccgagggcgc gctgaccgag gcgttgcgcg cggccacggg gctggtggtc
     3481 atcgcgcccg tagacggtta cagcccggcc gaggcccagg ccgtcagcga cctcgtcgcc
     3541 cgcggcgggc gcgtcctgct ggtgggcgac cccactcgct acaacatcga attcgacgag
     3601 accgacatct tcgccgcgcc gatcatcgcc acggcccagg caccgctcaa cgacctggcc
     3661 aacgccttcg acatcacctt ccgcggcgac tacctcta
//
LOCUS       DAOSMQ010000317         3696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25029_length_3696_cov_4.855809, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000317 DAOSMQ010000000
VERSION     DAOSMQ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25029_length_3696_cov_4.855809"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK829_10510"
     CDS             complement(<1..440)
                     /locus_tag="PK829_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOU41685.1"
                     /translation="MHGSSRAFPALVATLLAITLLAAGAAAWLRWGAARPVPATEPSA
                     AVATVALAAATVAPTLAPAATEGIGSLAGRPTQTAAPTGTTAAPTMTPSATSTPGVAE
                     YTVVDGDTLGAIAARFGITVAELVALNGLADPDQIAVGAVLRLPG"
     gene            complement(451..2550)
                     /locus_tag="PK829_10515"
     CDS             complement(451..2550)
                     /locus_tag="PK829_10515"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOU41686.1"
                     /translation="MTLSVGQVLENRYRVVSLLGQGGMGAVYRAWDLNLRVPVALKEN
                     LDLSLVSQQQFEREALLLAQLNHPNLPRVTNHFVIPGQGQYLVMEFIEGQDLQQWLDQ
                     RGKLTEAEAVPIVGQIASALAYLHSRPEPVIHRDVKPANIKITPDNRAILVDFGIAKQ
                     YTPQGHTTRGAQAITPGFSPPEQYGLGTTDARSDIYAVGATLYALLTGETPVESVSRL
                     AGATLPRPGHLTPSVARAILQAMEMEPQNRFQNAMAFAMALSSQVVDDKPPVDQRRLW
                     PVAAVAIFVLLTVAGFFVLRPLLSGRGGTATPDGSSGQEAKLDESSLATQTAIAVAQE
                     SLAASQSTATAAALTAVVEGDQDADGLSNDQEAALSTDPGDSDSDDDGLSDGDEVLIV
                     RSDPNNRDSDGDLLLDGDEVNVYATNPSQSDSDGDGDSDGLEVAQGSDPLSAAAPNLT
                     TPEPPSPTQTVKAKDAADLVEETIGFSAGGQPITLYKLGDGARVVTLVGGIHAGFAPG
                     GVAIVEQAMGHFVANPDEIPAGMALHFIPNSNPDSRLAVGELPGRVNANGVDVNRNFG
                     CDWSATAMWRNAPIDAGDGPFSEPESAALRDYFLDARPAVVIFYDARAAQGWVSPGEC
                     DGSMDDTVRFAEAYASASGYIYTVTDPIVGDASNWLVSQGTPAFFVLLRDYEEVTANT
                     LERNLDGIRDTIAVAAP"
     gene            complement(2566..2802)
                     /locus_tag="PK829_10520"
     CDS             complement(2566..2802)
                     /locus_tag="PK829_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU41687.1"
                     /translation="MTTSTCPHCTAVNRPGAIFCNACGRPLGSGPLPACAVRNAVLRP
                     GSRFCSRCGHRQDAAVVVEVTAGLLAVFYLLLLV"
     gene            complement(2812..>3696)
                     /locus_tag="PK829_10525"
     CDS             complement(2812..>3696)
                     /locus_tag="PK829_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU41688.1"
                     /translation="MGLGMALSFQELQRGAAPDYAARMILLTDGFTRDHNSCLAWTAR
                     AAEAGYAISTLGLGTEFNEDLLIPMAEQTGGQAHFVADPGGLPDIFRAELAAAQAVGF
                     RRAELKLQLTPGVEVRRITRVKPALSHMPTGPNAGGSYSIALGDMEWGAPPAFLLELI
                     IPPRGPGEYRLAQAVLSSDPVGAGRVTARADLVAQFAEGAAADVAADARVMGYVERVV
                     AFSLQTRALEEAAAGDLATATRRLHAAATRLLDMGETKLARTLQQQAEALDSRAALDA
                     TMTKQLQYATRQLTRKLE"
BASE COUNT          569 a         1243 c         1302 g          582 t
ORIGIN      
        1 cccggcagac gcagcactgc ccccacggca atctgatcgg ggtcggccag accgttgaga
       61 gccaccagtt ccgcgaccgt gatgccgaaa cgggcggcga tggcccccag cgtgtcccca
      121 tcgacaacgg tgtactcggc gacgcccggc gtggatgtgg ccgaaggcgt catcgtcggc
      181 gcggcggttg tgccggtcgg cgcggccgtc tgggtgggcc ggccggccag gctgccgatt
      241 ccctccgtgg ccgccggcgc gagggtgggc gcgacggtcg ccgcggccag ggcgaccgtg
      301 gccacggccg cggacggctc cgtggccggg acgggccggg ccgcgcccca acgcaaccac
      361 gccgccgcgc cggcggccag cagggtgatg gccagaagtg tggcgacgag ggccgggaaa
      421 gcgcgcgaac tgccgtgcat gctcataggg ttatggcgcg gccacggcga tggtgtcgcg
      481 gataccgtcg aggttgcgtt ccagcgtatt ggccgtcacc tcttcgtagt cgcgcagaag
      541 gacgaagaac gccggagtgc cctggctcac caaccagttg ctggcgtcgc cgacgatggg
      601 gtcggtgacg gtatagatat atccactcgc cgaagcgtag gcctccgcaa agcggacggt
      661 gtcatccatg ctgccgtcgc actcgcccgg cgacacccag ccctgggcgg cacgggcgtc
      721 gtagaagatg accacggccg ggcgcgcatc gaggaaatag tcgcgcaacg cagccgattc
      781 cggctcggag aatggcccgt cgccggcgtc gatgggcgcg ttgcgccaca tcgccgttgc
      841 gctccagtcg cagccgaagt tgcggttgac atcaacaccg ttggcgttga cgcggccggg
      901 cagctcgccc accgccagcc ggctgtcagg gttgctgttg ggaatgaagt ggagggccat
      961 gccggcggga atctcgtccg ggttggccac gaagtggccc atcgcctgct cgactatggc
     1021 gacgccgccg ggggcaaagc cggcgtggat gccgccgacc agggtgacga cacgcgcccc
     1081 gtcgcccagc ttgtaaagcg taatcggctg gccgccggcc gagaagccta tggtctcctc
     1141 gaccagatcc gccgcgtcct tcgctttgac tgtctgggtg ggcgagggcg gttcgggcgt
     1201 agtcaaattc ggcgcagcgg cgctgagcgg atcgctacct tgagcaactt caaggccgtc
     1261 gctatcgccg tcgccatcac tgtcgctttg gctcgggttc gtggcataca cattgacctc
     1321 gtcgccatcg agcagcaggt caccgtcgct gtcgcggtta ttgggatcgc tgcggacaat
     1381 cagcacctcg tctccgtctg acaggccgtc gtcgtcggag tcggaatcgc cgggatcggt
     1441 ggagagggcc gcttcctggt cgttgctcaa gccgtcagcg tcctggtcgc cctccacgac
     1501 ggccgtcagc gccgcggccg tggccgtgct ctggctggcc gccaggcttt cctgggcgac
     1561 agcaatggcc gtctgcgtgg ccaggctgct ttcgtccagc ttcgcctctt gacccgagga
     1621 gccgtcgggc gtggccgtgc cgccacgacc agacaaaagc ggccgcagca caaagaaccc
     1681 ggccacggtc agcaagacaa agatggccac cgcagccacc ggccagaggc ggcgctggtc
     1741 cacggggggc ttatcgtcta caacttgcga agacagcgcc atggcgaagg ccatcgcgtt
     1801 ttgaaagcgg ttctgcggct ccatctccat cgcctgcaag atggcacggg ccacggacgg
     1861 cgtcagatgg cctggccggg gcaacgtcgc cccggccagc cggctgacgc tctccaccgg
     1921 cgtctcgccc gtcagcaggg cgtagagcgt ggccccgacg gcatagatgt cggagcgggc
     1981 gtcggtcgtg cccaggccgt actgctccgg cggcgagaag ccaggcgtga tggcctgcgc
     2041 gccgcgggtg gtgtggccct gcggtgtgta ctgcttggca atgccgaagt ccaccaggat
     2101 ggcgcggtta tcgggggtga tcttgatgtt ggccggcttg acgtcgcggt gaatgaccgg
     2161 ttccggccgg ctatgtagat aggccagcgc cgaggcgatc tggccgacga tgggcacggc
     2221 ctcagcctcg gtcagcttgc cgcgctggtc gagccactgc tgcaagtcct gcccctcaat
     2281 gaactccatt accaggtact gcccctggcc ggggatgacg aagtggttgg tgacgcgagg
     2341 caggttgggg tggtttagtt gggccagcag gagagcctcg cgctcgaatt gctgctgtga
     2401 gaccagcgac aggtcgaggt tctccttcag ggcgacaggc acgcgcagat tgagatccca
     2461 ggcgcgatag accgcgccca tgccgccctg ccccagtagc gagacgacac ggtagcgatt
     2521 ctccagcact tgcccgaccg acagggtcat ctcttctctc cctcgctaga cgagcagcaa
     2581 cagatagaac accgccagca agccggccgt cacctccacc accacggccg cgtcttgcct
     2641 gtgcccgcaa cgagagcaga agcgcgagcc gggccgcagc acggcattgc ggacggcgca
     2701 ggcgggcaaa ggcccgctgc ccagcggccg gccacaggcg ttgcagaaaa ttgcccccgg
     2761 ccggttgaca gctgtgcagt gcggacacgt ggacgtggtc actaaagggc actactccag
     2821 tttgcgcgtc aactggcgcg tggcatattg tagctgcttg gtcatcgtgg cgtcgagggc
     2881 ggcgcggctg tccagcgctt cggcctgttg ttgcagcgtc cgcgccagct tggtctcgcc
     2941 catgtcgagc agccgtgtgg cggcggcatg cagccggcgc gtggccgtcg ccaggtcgcc
     3001 ggcggcggcc tcctccagag cgcgcgtctg caagctgaag gccaccacgc gctcgacgta
     3061 gcccatcacc cgcgcgtcgg cggctacgtc ggcggccgcg ccctcggcaa actgggcgac
     3121 gagatcggcc cgcgccgtca cccgccccgc gccgacgggg tcgctgctga gcaccgcctg
     3181 ggccagccgg tactcgcccg gcccgcgcgg cgggatgatc agttccagca gaaaggccgg
     3241 cggcgcaccc cactccatgt cgcccagggc gatggaatag ctgccgccgg cgttcggccc
     3301 cgtcggcatg tggctcagcg ccggcttgac gcgggtgatg cgccgcactt ccacgcccgg
     3361 cgtcaattgc agcttcagtt ccgcgcggcg aaagccaacg gcctgggccg cggccaactc
     3421 ggcgcggaag atgtcgggca gcccgccggg atcggccacg aagtgggctt gcccgcccgt
     3481 ctgctcggcc atggggatga gcaggtcttc gttgaactcc gtgcccaggc cgagggtgct
     3541 gatggcgtag ccggcctcgg ccgcccgcgc cgtccaggcc aggcagctat tgtggtcgcg
     3601 ggtgaagcca tcggtcagca ggatcatgcg cgcggcgtag tcgggcgcgg ccccgcgctg
     3661 caactcctgg aagctcaggg ccatgcccag gcccat
//
LOCUS       DAOSMQ010000318         3693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25062_length_3693_cov_4.340297, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000318 DAOSMQ010000000
VERSION     DAOSMQ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25062_length_3693_cov_4.340297"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..469
                     /locus_tag="PK829_10530"
     CDS             5..469
                     /locus_tag="PK829_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU41689.1"
                     /translation="MLIRTVSPNDRPEWLRLRHALWPDSPPDDEVGEVDTFLAGFPLP
                     TLMAAFVCERPEGGLCGLAEVAIHATAPGCVAGRVGYLEGWYVDPDCRGRGVGRALVA
                     AGEEWARAAGCREMASDTNPSYPVSPAAHAALGYEEVERFFRKELSGLETAD"
     gene            complement(444..1565)
                     /locus_tag="PK829_10535"
     CDS             complement(444..1565)
                     /locus_tag="PK829_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41690.1"
                     /translation="MSSDSSAPTPAGVEHIAAMSDPVARNHDITQCYHELAVAVARRL
                     PGGANWCAFATWASRQAGQSIRREDLRRTLARLLRESAEVEAAAGALESHAAAAGGPE
                     SLAGAVEAVREALSPAAAFDRAAAAVARGNLKVFAEIGRAFADYLALFDDGPPDAAAL
                     AAFLEQLRPGEPPEGQDYLRRAFAHMNDAMAASGRERAELLLLANVEIGFHEQTRLQP
                     EIRAAMDAPVYEPAELRRRLLAELFPNPVARLRLATLRLAGQAAPLLAARDRLADEAQ
                     RLGRLAITEVMMTLELPGGRALRLGHDLVGEYPAALRTVANADLRALLAGVGALGDSL
                     VGSSGADWSDLPGRMRYIADLFRAYHEDAALFNPPFPDR"
     gene            1623..1958
                     /locus_tag="PK829_10540"
     CDS             1623..1958
                     /locus_tag="PK829_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOU41691.1"
                     /translation="MTTFACRSGCGACCIAPSISSPIPGMPHGKPAGVRCVQLTDDNL
                     CRLFGLPDRPAVCVNLRPSAEMCGETREEALLYLERLELLTRPNPLCDNRNRDYLLEQ
                     WHEFKGRGS"
     gene            2198..>3693
                     /locus_tag="PK829_10545"
     CDS             2198..>3693
                     /locus_tag="PK829_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOU41692.1"
                     /translation="MKRLLILMSLALLVVLAACGGQTPQVEIQPTVEIAGETTEAGVV
                     EAVPTEALPAETSPIAHVADPALVNKEWLWESRDPNGGATAAITVPTPEEYNIIFNED
                     GTFFAQLDCNRGSGDYATPGDGSLTMTLGMTSRAMCPPDSLSNQMIELFASAASYTIQ
                     EDGAVLAIAWANGGPIDTYRAADVMTTTTTGMDAALVGKKWQWLGTTTPVEEVTVADA
                     SRYTIEFLEDGSAAITADCNTVLAQVTADGTNMTITPGPSTLVACPEDSQADLFTQQL
                     SAAAVYFFQDGDLFIDLFASSGTMHFEEVPLVDLPAPDAGEPTGTVNAPAGVFLRSGP
                     GVNFPAIGTAAQGDSGTLIGISQDRNWFVVEAPNMPDGRVWVAAAFVDAVGAENLPTV
                     QAPALPSTLVGPVWQWAGTAGAQATTVADPTRYTITFMANGTASIKADCNMVTAAYTL
                     TGTGLGLVPGASTMAMCPPDSQGQQFVQQLALATVHTVQGSELMLNLT"
BASE COUNT          597 a         1326 c         1212 g          558 t
ORIGIN      
        1 taaaatgctc atccgcaccg tatcccccaa cgaccgcccg gaatggctgc gcctgcgaca
       61 cgccctgtgg cccgactcgc caccggacga cgaggtcggg gaggtcgata ccttcctggc
      121 cggcttccct ttgccgacgc tgatggccgc gttcgtctgc gagcggccgg agggcggcct
      181 gtgtgggctg gcggaggtgg ccatccacgc cacggccccc ggctgcgtcg ccggccgcgt
      241 cggctatctg gagggctggt acgttgatcc cgactgccgc ggccggggcg tggggcgggc
      301 cctggtcgcg gccggcgagg agtgggcgcg ggcggccggc tgccgcgaga tggcctccga
      361 caccaacccg tcctaccccg tcagcccggc ggcgcacgcc gctctcggct acgaggaagt
      421 ggaacgcttc ttccgcaagg aactcagcgg tctggaaacg gcggattgaa cagggccgcg
      481 tcctcgtggt aggcgcggaa caggtcggcg atgtagcgca tccggccggg caggtcgctc
      541 cagtccgcgc cgctgctgcc caccaggctg tcgcccagcg cgcccacgcc cgccagcagc
      601 gcccgcaggt cggcgttggc caccgtccgc agcgcggccg ggtactcgcc cacgagatcg
      661 tgaccgaggc gcagtgcccg gccgccgggt agctccagcg tcatcatcac ttcggtgatg
      721 gccaggcggc ccagccgctg cgcctcgtcg gccaggcggt cgcgggcggc cagcagcggc
      781 gcggcctgcc ccgccagccg cagggtcgcc aggcgtagcc gcgccaccgg gttcgggaat
      841 agctccgcca gcagtcgccg gcgcaactcg gccggctcgt acaccggcgc gtccatcgcc
      901 gcgcgaatct ccggttgcag ccgtgtctgc tcgtggaagc cgatctccac gttggccagc
      961 agcagcaact cggcccgctc ccggccactc gccgccatcg cgtcgttcat gtgggcaaag
     1021 gcgcggcgca gatagtcctg tccttccggc ggctcgccgg ggcgcagctg ctccaggaag
     1081 gccgcgaggg ccgcggcgtc gggcgggcca tcgtcgaaca gggccaaata gtcggcaaag
     1141 gcccggccga tctcggcgaa gaccttcagg ttgccgcggg ccacggccgc ggcggcgcgg
     1201 tcgaaggctg ccgccgggct gagcgcctcg cgcacggcct cgactgcccc ggccaggctc
     1261 tccggcccgc cagccgcggc ggcgtggctt tccagcgcgc cggcggccgc ctcgacctcg
     1321 gctgactccc gcagcagccg cgccaacgtc cgccgcaagt cctcgcgccg gatcgactgg
     1381 cccgcctgcc gcgacgccca ggtggcgaag gcgcaccagt tggccccgcc cggcagccgg
     1441 cgagccacgg ccacggccag ttcgtggtag cattgggtaa tgtcgtggtt acgggccacc
     1501 gggtcagaca tggccgcgat gtgctcgacg ccggccggcg tgggcgcgga actgtcgcta
     1561 ctcatacggc tatgatacgc gccccagccc cacgcgccat tcttcgcgct ataatcagac
     1621 gcatgacgac cttcgcctgt cgctccggct gcggcgcgtg ctgtattgcc ccatcgatct
     1681 cctcgcccat ccccggcatg ccccacggca agccggccgg cgtgcgctgc gtgcagttga
     1741 ccgacgacaa tctctgtcgg ctgttcggct tgcccgaccg gccggcggtg tgcgtcaacc
     1801 tgcggccgag cgccgagatg tgcggcgaga cgcgcgagga ggcgttgctc tacctggaac
     1861 gccttgagct tctgacgcgc cccaatcctt tatgcgataa tcgcaatcgg gactatttac
     1921 tggagcaatg gcatgagttc aaaggccgag gcagctgaaa ccgagttggc gagagtaggc
     1981 ggcgcctacg cgagacctac cagtgtcaag tctaccactg tgccgccccg ccgatggcta
     2041 gacaacatga tccgtaaatg agcgccacgt aagaagcatg gcctcatcgg aaccccattc
     2101 aacactagac gttaatctat agagagctta gctcccgtta aagtcgcgcg gcatgcgcgg
     2161 cacattgctg aacacattgc agaggagatg aacgtcgatg aaacgcttac tgattttgat
     2221 gagtctcgcc ttgctggttg tcctggcggc ctgtggcggc cagacgccac aagtagagat
     2281 tcagccgacg gtcgagatcg ctggtgaaac caccgaagcc ggggttgttg aggcagtgcc
     2341 caccgaggcc ctgccggccg agacctcgcc catcgctcat gtcgctgacc cggcgctggt
     2401 taacaaggag tggctgtggg agagccgtga tcccaatggc ggcgcgaccg cggccatcac
     2461 cgtacccacc cctgaagagt acaacatcat cttcaacgaa gatggcacct tcttcgccca
     2521 gctggactgc aaccgcggca gcggcgacta cgccacgccc ggcgacggca gcctgaccat
     2581 gaccctaggc atgaccagcc gggccatgtg cccgcccgac tcgctgtcga accagatgat
     2641 cgagctattc gcctctgcgg ccagctacac catccaggaa gacggcgcgg tgctggccat
     2701 cgcctgggcc aacggcgggc cgatcgacac ctaccgcgct gccgatgtca tgacaacgac
     2761 caccaccggc atggacgcgg cgctggtggg caagaagtgg caatggctgg gcacgacgac
     2821 gcccgtagaa gaggtcaccg tcgccgacgc ttcgcgctac acaatcgagt tcctcgaaga
     2881 cgggtcggcg gccatcaccg ccgactgcaa caccgttctg gcccaggtaa cggccgatgg
     2941 caccaacatg accattaccc ccggcccgtc cacgcttgtg gcttgcccgg aggattcgca
     3001 ggccgacctg ttcacccagc agctgagcgc ggcggcagtc tacttcttcc aggatggcga
     3061 cctcttcatc gacctgttcg ccagcagcgg cacgatgcac ttcgaggaag tgccgctggt
     3121 tgacctgccc gcgcccgatg ccggcgaacc gaccggcacg gtcaacgccc cggccggcgt
     3181 cttcctgcgc agcggcccgg gcgtcaactt ccccgccatc ggcacggcgg cgcagggcga
     3241 cagcggcacg ctgatcggca tcagccagga tcgcaactgg ttcgtcgtcg aagcgccgaa
     3301 catgcccgac ggccgcgtct gggtggcggc ggccttcgtt gatgccgttg gcgctgagaa
     3361 tctgcccacc gtccaggccc cggcgctgcc ctcgacgctg gttggcccgg tgtggcagtg
     3421 ggccggcacg gccggggcgc aggctaccac cgtcgccgac ccgacccgct acacgatcac
     3481 cttcatggcc aacggcacgg ccagcatcaa ggccgactgc aatatggtca cggccgccta
     3541 cacgctgacc ggcaccggcc tcggcctcgt ccccggcgcg tcgacgatgg ccatgtgccc
     3601 gcccgactcg caggggcagc agttcgtgca acagctggcc cttgccaccg tccacacggt
     3661 gcagggcagc gagctgatgc tcaacctgac cga
//
LOCUS       DAOSMQ010000319         3690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25093_length_3690_cov_3.650894, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000319 DAOSMQ010000000
VERSION     DAOSMQ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25093_length_3690_cov_3.650894"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PK829_10550"
     CDS             <1..1091
                     /locus_tag="PK829_10550"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41693.1"
                     /translation="APSWSGSGYDLTAGAYVGTIALRDAAGGLLDIVIEPKAPVDNLF
                     YMLTYAHRLVEFGAHASPLLAGADIFDSVVDIFVRQVDDIARRGIYRGYIDREESAAF
                     LRGRLLVAPQVRRGPLAVTFHQRTNEFTADLLENRILSAALWLLAQARFRDPNLGPRV
                     RRALAAFGEASPVGITAAHCDRVVYSRLTERYRSPINLARLFLRHLSLEGQAGATPFA
                     SFLVPMPRLFEQFVARLLAEQLSAWPRLRLEDQWRIYLDEERQLEGRPDLLLYRDGRA
                     ALVLDTKYKVYNEKPTNADINQMVTYCHTLGIDRGVLLYPGDTLGDEYQLKAGVTLTI
                     RGLSLTGPLAAFRARARAFAHDLAASYS"
     gene            1125..3095
                     /locus_tag="PK829_10555"
     CDS             1125..3095
                     /locus_tag="PK829_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007368243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41694.1"
                     /translation="MTDSRVRSYWLEWLILLAVVYLYSHAATLDFDPQQMQQTGEQNE
                     SATLPILAELGFRHGEIPLWNPYMLTGFPHTGDPVSHFWNPVATLPVLLWGGINGMKV
                     SLFLSLLVAAFGQWYLAHVFGLRGVFRLWAGLLFVLSGGLAMLSWLGWYELLVGAAWF
                     PWCFALLWRALRRGGRLSVVLAAGAIAMVITTGGGYYPLYLGVCLAVLTGMALVWSRL
                     GEHGRVLRRAAAVAVLAAGLSAVYWLPLVDGLRYTVRDAPPDGMQIGSQAIPYALFNF
                     VVSDPTWFRAEVLNKGSGSGWLYVGLLPIVALGLLPWVYAHFRGRRRGLLTLLALLLT
                     LLLWNANRYAPVRTLYDLLPFLYTFRFPGRLLIIATSPLLVLAGMGLQTLLVRARRRV
                     PARAEAGGITPRWLMGVAAALLLLLSVINVFRVNQPFMTAPHPRNQVARAALTWLREA
                     DPGLYYINIGGDIIYWDWLAYAYELELPVLNFRYNRGLDSMDAQYGPLSPFNAQPKYT
                     FVVASEPPPPGATQVNLFAEVAVWRRDDVLPYAFAARPDAPITTDTVRAVDVRLDGPN
                     RVVVQAAAEADETQLVVLVSDYPGWRLTVDGAPAELQPLNGYLGAALRPGAHTYVFAF
                     RPPLHAVALAVSGLSLLACLALLAPALRRPKV"
     gene            complement(3216..>3690)
                     /locus_tag="PK829_10560"
     CDS             complement(3216..>3690)
                     /locus_tag="PK829_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41695.1"
                     /translation="QVSQTLGANEQFGDLLFSADSESVFYSVYPNMDYHLALYRAHAD
                     GRGDPQRLGDPAARIDSWDVAPSGDRILYTAPAGSGTSYELYSAAGDGHGVAVRLSGP
                     MVAGGNVSSFRFSPDESRVVYMADQNTDEVEELYGAIVPAVQPETLQLFLPVTVR"
BASE COUNT          491 a         1299 c         1216 g          684 t
ORIGIN      
        1 ttgcgccgtc gtggtcgggc agcggctatg acctgaccgc cggcgcttac gtcggcacca
       61 tcgccctgcg cgacgccgcc ggcgggctgc tggacatcgt catcgagcca aaagcgcccg
      121 tcgataacct gttctacatg ctgacctatg cccaccggct tgtcgagttc ggcgcgcacg
      181 cttcgccgct actggccggg gcggacatct tcgattcggt cgtggacatc ttcgtgcgcc
      241 aggtggacga catcgcccgg cgcggcatct accgcggcta catcgaccgg gaggagtccg
      301 ccgcctttct gcgcggccgg ctgctcgtgg ctccccaggt gcgccgcggc ccactggcgg
      361 tgacgttcca ccagcggacg aatgagttca ccgccgatct gctggaaaac cgcatcctga
      421 gcgcggcgct ctggctgctg gcccaggcgc gcttccgcga ccccaatctg gggccgcgcg
      481 tgcggcgggc actggccgcc ttcggcgagg cgtcgcccgt gggcatcacc gcggcccatt
      541 gcgaccgcgt cgtctactcg cgcctgacgg agcgctaccg ctcgcccatc aatctggcgc
      601 ggctgttcct gcgccacctg tcgttggagg ggcaagcggg cgcgaccccc ttcgcgtcct
      661 tcctggtacc catgccgcgc ctgttcgagc agttcgtcgc ccggctgctg gccgagcagc
      721 tatcggcctg gccccggttg cggttggaag accagtggcg catctatctc gacgaggagc
      781 gacagttgga ggggcggccc gacttgctcc tctaccgcga cggccgcgcc gcgctcgtgc
      841 tggacacgaa gtacaaggtc tacaacgaga agccgaccaa cgccgacatc aaccagatgg
      901 tcacctactg tcacacgctg ggcatcgacc gcggcgtgct gctctacccc ggcgacaccc
      961 tcggcgacga gtaccagctg aaagcaggtg tgactctgac aattcgcggg ctgtcgctga
     1021 ccggccccct ggccgcgttc cgggcgcgcg cccgagcctt tgcacacgac ctggccgcga
     1081 gctacagtta gcgcgtggcg gccacccggc cgcgctcgcc aagcatgacc gactcccgcg
     1141 tccgctccta ctggctcgaa tggctgatcc tgctggccgt ggtctacctg tacagccacg
     1201 ctgccacgct cgacttcgac cctcagcaga tgcaacagac cggcgaacag aacgagagcg
     1261 ccaccctgcc catcctggcc gagttgggct tccgtcacgg cgagatcccg ctctggaacc
     1321 cctacatgct gaccggcttt ccccataccg gcgatcccgt cagccacttc tggaacccgg
     1381 tggcgacgct gccggtgctg ctgtggggcg gcatcaacgg tatgaaggtg tcgttgttcc
     1441 tgtcgctgct ggtggccgct ttcgggcagt ggtatttggc ccacgtcttc ggcctgcgcg
     1501 gcgtcttccg gctgtgggcg gggctgctgt tcgtcttgtc gggcgggctg gccatgttgt
     1561 cgtggctggg gtggtatgag ctgttggtcg gcgcggcctg gttcccctgg tgcttcgccc
     1621 tgctctggcg ggcgttgagg cggggcggtc gcctatctgt ggtgctggcc gccggagcca
     1681 tcgccatggt catcaccacc ggcggcggct actacccact ctacctgggc gtgtgcctgg
     1741 cggtgctgac gggcatggcc ctggtctgga gccggttggg ggagcatggg cgggtgctgc
     1801 gccgggcggc ggccgtggcc gtgctggcgg ccgggctgtc ggccgtctac tggctgccgc
     1861 tggtcgatgg cctgcgctac accgtccgcg acgccccgcc cgacggcatg cagatcggct
     1921 cgcaggcgat cccctacgcc ctgttcaact tcgtcgtctc cgacccgacc tggttccggg
     1981 ccgaggtgct caacaagggt agcggcagcg gctggctcta cgtcggcctg ctgcccatcg
     2041 tcgcgctggg gctgttgccg tgggtctatg cccacttccg cggccggcgg cgcggcctgc
     2101 tgacgctgct ggcgttgctg ctgacgctgc tgctctggaa cgccaatcgc tacgcgccgg
     2161 tgcgcactct ctatgacctg ctgccgttcc tctacacctt ccgctttccg ggacggctgc
     2221 tcattattgc tacgtcgccg ctactggtgc tggcgggcat gggcctgcaa acgctgctgg
     2281 tgcgcgcccg ccggcgcgtg cccgcccgcg ccgaggcggg tggcatcacc ccgcgctggc
     2341 tgatgggcgt cgccgccgcg ctcttgttgc tgctgtcggt catcaatgtt tttcgcgtca
     2401 atcaaccctt catgaccgcg ccccaccccc gcaaccaggt cgcccgcgcg gcgctgacct
     2461 ggctgcgcga ggccgatccg gggctgtact acatcaacat cggcggcgac atcatctact
     2521 gggactggct ggcctacgcc tatgagctgg aactgccggt gttgaacttc cgctataacc
     2581 gcggcctgga cagcatggac gcgcagtacg gccccctgtc gccgttcaat gcccagccca
     2641 agtacacctt cgtcgtggcc tcggagccgc cgccgccggg ggcgacacag gtgaacctgt
     2701 tcgcggaggt ggctgtctgg cggcgcgacg acgtgctgcc ctacgccttc gccgcccggc
     2761 cagacgcgcc catcaccacg gacacggtgc gcgccgtcga cgtgcgactg gacgggccga
     2821 accgggtcgt cgtgcaggcc gcggccgagg cagacgagac gcaactggtc gtcctcgtca
     2881 gcgactaccc cggctggcgg ctgacggtcg atggcgcgcc ggccgaatta cagccgctca
     2941 acggctatct gggcgcggcg ctgcggccgg gggcgcacac ctacgtcttc gccttccgcc
     3001 cgcctctcca cgcggtcgcc ctggccgtca gtggcctgtc gctattggcc tgtctggccc
     3061 tcctggcgcc ggccctccgg cgaccaaagg tgtaagaact taacgcaagg acgcaaagaa
     3121 gcaaggacgc aaggaagaaa tagcttcctt gcgtccttgc ccctttgcgt ccttgcgttg
     3181 acttcttcct tgcgtccttg cgttaacttc tgctcttacc gcaccgtcac cggcaggaac
     3241 agttgcagcg tttccggctg cacggccggc acaatggctc catacagctc ttcaacctca
     3301 tcggtattct ggtcggccat gtagacaacg cggctctcgt cggggctgaa gcggaagctc
     3361 gacacattgc caccggcgac catcgggccg ctgagccgga cggccacgcc gtggccgtca
     3421 ccggcggcgc tgtagagttc gtagcttgtc ccgctgcccg ccggggccgt gtagaggatg
     3481 cggtcgccgc tgggggccac atcccaactg tctatccggg ccgcgggatc gccgagccgc
     3541 tgtgggtcgc cgcggccgtc ggcgtgggcg cgatagagcg ccaggtggta atccatgttc
     3601 gggtagacgc tgtaaaagac gctctcgctg tccgcgctga agagaaggtc gccgaactgc
     3661 tcgttcgcgc cgagcgtctg gctcacctgg
//
LOCUS       DAOSMQ010000320         3669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25282_length_3669_cov_4.143885, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000320 DAOSMQ010000000
VERSION     DAOSMQ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25282_length_3669_cov_4.143885"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PK829_10565"
     CDS             <1..738
                     /locus_tag="PK829_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HOU41696.1"
                     /translation="HESGHFILPNPWDIGGIRRLEKLGFKALASTSAGYAWSLGREDQ
                     ELTLEETLAHLRLLCAATDLPINADFENGYAHQPEDVATNVALALETGVAGLSIEDLD
                     GQALYPLDLAVERIRAARAAIDQIAPEAMLVGRSEGYRIGQKDVRATIERLVAYAGAG
                     ADVLYAPLLDDIDEIRAVVTAVAPKPLNVLLSYPGMKAPMNVPALAAAGVRRSSVGGW
                     LARYAGMAFERAALTLRDTGFLPEQGR"
     gene            735..1196
                     /locus_tag="PK829_10570"
     CDS             735..1196
                     /locus_tag="PK829_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU41697.1"
                     /translation="MNRQQLLQKLDKAWLALADSFAGLSEAQMTEPGVSGEWSVKDIL
                     GHVTAWEQEALTHLPHIAAGQRPPRYADVYGGIDAFNALRAGENHARPLDEVLARFGA
                     VHGQLVAAIEAAPEELLTTETRYRRRIRLDTYSHYPVHAEAIREWRERRGY"
     gene            1234..2262
                     /locus_tag="PK829_10575"
     CDS             1234..2262
                     /locus_tag="PK829_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOU41698.1"
                     /translation="MIAVVLLAAGQGTRMNSRKQKILHEVGGRPMVLHAFLAAAAVAD
                     RPPVLVIGPGETGVPALLGDRAEYVVQPEALGTGHATMMAADVLRGCADQVIVAYGDM
                     PLLRAETLRRLAETQRAAGAAVALLSVPGPPDSAFGRVVRDAAGGVVAIIEVAEARRR
                     PDAEALLAISELNAGVYCFDGDWLWANIDRLPVRQARRGQEYYLTDMVELAVAAGQPV
                     AAVMAGDPDEGLGAGTRAEMVDVERAFRRRVNAHWLAHGVTLVEPEATYIGPDVTIGQ
                     DTIIWPNTFLQGQTTIGTECVIGPNAILRDTRVGDGSRVEQLLLDGQCVPDGAIARPG
                     QWQPTGEA"
     gene            2259..2678
                     /gene="cdd"
                     /locus_tag="PK829_10580"
     CDS             2259..2678
                     /gene="cdd"
                     /locus_tag="PK829_10580"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOU41699.1"
                     /translation="MSETTITAEQRDQLIAAACAARARAYARYSRFHVGAAILSDDGR
                     FYTGVNIENASYGLTVCAERNAVGAMVNDGALRIAAVAVCTENGVTPCGACRQVLSEF
                     VDHESDIPVWIIDGRGEVRETSLAALLPDSFGARHLA"
     gene            2718..>3669
                     /locus_tag="PK829_10585"
     CDS             2718..>3669
                     /locus_tag="PK829_10585"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOU41700.1"
                     /translation="MSGPYIYTTAIWPPILTALFLLALAVYSGRRRSVPGALPFAASG
                     VFSALWAASYALQYAATDPLTKTFWLRFGLAWSLPALTAITCFIVEYAWPGRWLTRRT
                     LALLAIVPLLFVLLLASRLHPLLIRDYVVTDEVLAQLAPGSWVFFIYAYLLGLLNIGV
                     LIWLFVRSPQQRGLATLLMAGQVISRAFYAQQLLSADHLDLPFNAPIMIVPYTIYAVA
                     LFAFRIFDPMVMARQSAIDQLDAGVLVLDRAGRVVGHNPAAANMLGLPGEEIRGRPIS
                     TLLPAGAAETIHSAIAAGSSDFHALSLRERDVRLQTSPLRD"
BASE COUNT          563 a         1242 c         1235 g          629 t
ORIGIN      
        1 catgaatcgg gtcacttcat cctgcccaat ccgtgggata ttggtggcat tcgccggctc
       61 gagaaattgg gctttaaggc cctggcctcg accagcgccg gctacgcctg gtcgctcggc
      121 cgcgaagacc aggaactgac cctggaggag accctggccc atttgcgcct gctgtgcgcg
      181 gccacagacc tgcccatcaa tgccgacttc gagaacggct atgcccacca gcccgaagat
      241 gtggccacca atgtcgcctt ggccctcgag acaggcgtgg ccggcctatc gatcgaggac
      301 ctcgacggcc aggcgctcta tccgctcgac ctggccgtgg agcgcatccg cgcggcccgt
      361 gcggccattg accagatcgc gccagaggcg atgcttgtcg gccggtcgga aggttatcgc
      421 attgggcaga aggatgtgcg cgcgacgatc gagcgcctgg ttgcctatgc gggcgcgggc
      481 gccgatgtcc tctacgcgcc cctgctagat gacatagacg agatacgggc cgttgtcacc
      541 gcggttgccc ccaagccgct caacgtcttg ctgagttacc ccggtatgaa agcgccgatg
      601 aacgtccccg cgttagccgc cgccggtgtt cgccgcagta gcgttggcgg ttggctggcc
      661 cgctatgccg ggatggcgtt cgagcgggca gcgctgacgc tgcgcgacac cggctttctg
      721 cccgaacagg gtcgatgaac cgacaacaac tcttgcagaa actagataag gcctggctgg
      781 cgctggccga ctctttcgcc gggctgagcg aggcgcaaat gaccgagccc ggcgtcagcg
      841 gcgagtggtc ggtcaaggac atcctgggtc atgtgaccgc gtgggagcag gaggcgctga
      901 cgcacctgcc ccacatcgcc gccgggcagc ggccgccacg ctacgccgat gtctatggcg
      961 gcatcgacgc cttcaacgcc ctgcgggccg gggagaacca cgcccggccg ctggacgaag
     1021 tgctggcgcg cttcggggcc gtccacggcc aactggtggc cgcgattgaa gccgcgccag
     1081 aagagttgct gaccacagag acgcgctacc gccggcgcat tcgcctcgac acctacagcc
     1141 actatcccgt ccacgccgag gccatccgcg agtggcggga gcggcgcggc tactgattcg
     1201 tccggttacc acagccacca gccagtcacc gccatgatcg ccgtcgtcct cctcgccgcg
     1261 ggccagggaa cccgcatgaa ctcccgcaag cagaagatcc tgcacgaagt cggcggccgg
     1321 ccgatggtgc tccacgcctt tctggccgcc gcggccgtgg ccgaccggcc gccggtgctg
     1381 gtcatcgggc cgggagagac gggcgtgccg gcgctgctgg gcgaccgggc cgaatacgtc
     1441 gtccagccag aagcgctggg cacgggccac gcgacgatga tggccgccga tgtcctgcgt
     1501 ggctgcgccg atcaggtcat cgtcgcctat ggtgacatgc ccctgttgcg cgccgagacg
     1561 ctgcggcgac tggccgagac gcagcgcgcg gcgggcgcgg ccgtagcctt gctgagtgtg
     1621 cccggcccac cggacagcgc cttcggccgc gtcgtccgcg acgccgccgg cggcgtggtg
     1681 gcgatcatcg aggtagccga ggcccgtcga cggccggacg ccgaggcgtt gctggccatc
     1741 agcgaactga acgcgggcgt ctactgcttt gacggcgact ggctgtgggc caatatcgac
     1801 cggctgcccg tgcgccaggc gcggcggggg caggagtatt atctgaccga catggtcgaa
     1861 ctggccgtgg ccgcggggca gccggtggcg gcggtcatgg ccggcgaccc cgatgaaggg
     1921 ctgggcgcgg gcacgcgggc ggaaatggta gacgtggagc gcgccttccg ccggcgcgtc
     1981 aacgcccatt ggctggccca cggcgtgacg ctggtcgagc cggaggcgac ctacatcggt
     2041 ccggacgtga ccattggcca ggacacgatc atctggccca acaccttctt gcagggacag
     2101 acgaccatcg gcacggaatg tgtcatcggg ccaaacgcca tcttgcgcga tacacgcgtc
     2161 ggcgatggca gccgggtgga gcagctgttg ctcgatggcc agtgcgtgcc tgatggggca
     2221 atcgcgcggc cgggtcaatg gcagccgaca ggagaagcat gagcgagaca acgatcactg
     2281 cggaacaacg cgaccagctc atcgccgcgg cctgcgccgc gcgcgcccgg gcctatgccc
     2341 gctattcgcg cttccacgtc ggcgcggcca ttctcagcga cgacggccgt ttctataccg
     2401 gcgtcaacat cgagaacgcc tcctatggcc tgaccgtctg cgcggagcgc aatgccgtgg
     2461 gagcgatggt caacgatggc gcgctgcgga tcgccgccgt ggccgtctgc accgagaacg
     2521 gcgtgacgcc ttgtggcgcg tgccggcagg tgctgagcga gttcgttgac cacgagagcg
     2581 atattcccgt gtggatcatc gatggccgcg gcgaggtgcg ggagacttcg ctggcggcgc
     2641 tgctgccgga cagcttcggg gcgcggcatc tggcctaaag agggggcgtg ggttggctgc
     2701 ggggttcagg agggacgatg tcggggccgt acatctacac caccgccatc tggccgccca
     2761 ttctgacggc gctcttcctg ctggcgctgg cggtctatag cgggcgtcgc cgcagcgtac
     2821 cgggggcgct accctttgcc gccagtggcg tgttcagcgc cctgtgggct gccagctacg
     2881 ccctgcagta cgccgcgacc gatcccctga ccaagacctt ctggttgcgc ttcgggctgg
     2941 cctggtcgct gccggctctt accgctatca cctgtttcat cgtcgaatac gcttggcccg
     3001 gccgctggct gacgcgccgc actctcgccc tgctggcgat cgtgccgctg ctatttgtgc
     3061 tgctgctggc gagccggctt catcccctgc tgattcgcga ctatgtggtg actgacgaag
     3121 tcctcgccca actggctccc ggcagctggg tctttttcat ctatgcctat cttctgggcc
     3181 tcctgaacat cggcgtgctc atctggctgt tcgtccgctc gccccagcaa cgcgggctgg
     3241 ccacgctcct gatggccggt caggtcatta gccgggcctt ctacgcgcag cagttgctca
     3301 gcgccgatca cttagacctg cccttcaatg cgccgatcat gatcgtgccc tacaccatct
     3361 atgccgtggc gctcttcgct tttcgcatct tcgacccgat ggtcatggcc cgccagtcgg
     3421 ccatcgacca actggacgcc ggcgtgctgg tgctcgatcg cgccggtcgc gtggtcggcc
     3481 acaacccggc cgcggcgaac atgctagggc ttcctgggga agagattcgc gggcgtccaa
     3541 tcagcacatt gttgcccgcc ggcgcggccg agaccatcca cagcgcaatt gccgccggca
     3601 gcagtgattt ccacgccctg tccctgcgcg aacgcgatgt ccggttgcag acctcgcccc
     3661 tgcgagact
//
LOCUS       DAOSMQ010000321         3663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25335_length_3663_cov_4.151608, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000321 DAOSMQ010000000
VERSION     DAOSMQ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25335_length_3663_cov_4.151608"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2404
                     /locus_tag="PK829_10590"
     CDS             <1..2404
                     /locus_tag="PK829_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41701.1"
                     /translation="LGVSPDPPGQALSGPAFAQQLGTCLEAFWQARAVAGPVAIILDD
                     LQWIDASSAALVAQLFGLSESTAVLFLCAMRRERHSDGWRLRDAAHRDYPHRFDEAAL
                     YPLTDSDSLMLLDGLLDGSALPEPYRRTILDKAEGNPLFVEEVVHNLIERGHLQREAE
                     GAPWTAAGSPDDIELPDSLQALLTARIDRLEENTRRVLQIASVVGRTFTRSALLPLVD
                     DPNGLDRQLLDLQRAELVREVGRVPEPEYTFHHNLTHEATYNTILVKERRALHLRVAA
                     ALEGASGSQASLAHHYLEGNAPERALPCLVAAADKALRLNATVEALAHYGRALPIALA
                     GADATLLGHLYVARGRALELTSQFVEADAVYAELEALARERGVPSLELAALIAQGTLR
                     ANVTPLRDAPAARALMERALALAEALDDRPAEVRILWNLLNIDRFDIHNLPQSVNEGA
                     RGLALARELDLKEETAYLLNDLGEALGSLGRMTEAHDMLAEAVQRWRALGNEPMLIDG
                     LSGQANWALFTGDLPRARTLADEAYERSLRIDNPWSQAYSGGVSGLLYALQGEIGAGL
                     DRLYRSIARAEEAGFVGGLILMRAFVSKALLAIGAVDEAAAVATEGLAIGRAQLPQFA
                     GMCLAQLALISLRTGDTATAAELLEDPLLQVEHLQAFVEIDAKLGWIALATAQGRTEE
                     ALRQITALKARAQEMGTIFWLPEALQAEAAVLLTLDRASEAAGALAAAVDLSRRSGAR
                     FLLWVYLSQLADAQDKLGDGRAPETRAAAAAELATIRQHTWPDALRESLARWAATISP
                     RL"
     gene            2559..>3663
                     /locus_tag="PK829_10595"
     CDS             2559..>3663
                     /locus_tag="PK829_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU41702.1"
                     /translation="MPAISDSAQLRAAMTAIESQRLVLGDAVVDAALVAMRAQLVALD
                     AAPAAEQQRKQATVLFADVVGFTTLAETKDAEEVTELINALWAQLDGLITAYGGRIDK
                     HIGDAVMALWGVESAREDDPERAIRAALAMQEEISAFNAAMKNRRRLPDDLALELRVG
                     INTGPVLLGQVGLTREYTAMGDTVNLANRLEQTAPMGGVLVAHNTFRHVRGVFDARSL
                     GPIQVRGRHEAVLAYVVLRPKPRAFRLATRGVEGIETRTIGRDAELAALQAAFAASIA
                     EARARIVAVVGEAGVGKSRLLYEFDNWVDLRPERVRYFKGRATPALQGVPFSLWRDVF
                     AFRFDILDSDSPSVALNKFRSGFVSHRAAAEATA"
BASE COUNT          545 a         1354 c         1217 g          547 t
ORIGIN      
        1 gctgggcgtc tcgcccgacc cgcccggcca ggcgctgagc ggcccggcct ttgcccagca
       61 actgggcacg tgtctggagg ctttctggca ggcgcgcgcc gtggccgggc cggtggccat
      121 catcctcgac gacttgcagt ggatcgacgc ctcctcggcc gccctcgtgg cccagctttt
      181 cgggctgagc gagagcacgg cggtgctgtt cctgtgcgcc atgcggcgcg agcgccactc
      241 cgacggctgg cggctgcgcg acgccgccca ccgcgactat ccccaccgct tcgacgaagc
      301 cgccctctat cccctgaccg acagcgacag cctgatgctg ctcgacggcc tgctcgacgg
      361 ctccgcgctg ccggaaccgt atcgccgcac catcctcgat aaggccgagg gcaaccccct
      421 tttcgtcgaa gaggttgtcc ataacctgat cgagcgcggc cacttgcagc gtgaggccga
      481 gggcgcgccc tggacagcgg ccggctcgcc cgacgacatc gaattgcccg attcgctgca
      541 agccctgctg acggcgcgga tcgaccggct ggaagagaac acccggcgcg tcttgcagat
      601 cgcctcggtc gtcggccgta ccttcacccg ctcggccttg ctccccctgg tcgatgaccc
      661 caatggcctt gaccggcaac tgctggactt gcagcgcgcc gagctagtgc gcgaggtcgg
      721 ccgcgtgccg gagccggagt acacgttcca ccacaacctg acccacgaag cgacctacaa
      781 caccattctc gtcaaggagc ggcgggcgct gcacctgcgc gtggccgcgg cgctggaagg
      841 cgcgtccggc agccaggcgt cactggccca ccactacctg gagggcaacg ccccggagcg
      901 cgccctgccc tgtctcgtcg ccgccgccga taaggccctg cgtctcaatg ccaccgtcga
      961 ggccctggcc cactacggcc gcgccctgcc catcgccctg gcgggggcgg acgcgacgtt
     1021 gttgggtcat ctctacgtcg cgcgcggccg ggcgctggag ctaacgtcgc aatttgtgga
     1081 ggccgatgcc gtctacgccg agctggaagc cctggcgcgc gagcgcggcg taccgtcgtt
     1141 ggaactggcc gcgctcatcg cccagggcac actgcgcgcc aacgtcaccc ccctgcgcga
     1201 cgccccggcg gcgcgagcgt tgatggagcg ggcgctggcg ctggccgagg cgttggatga
     1261 ccgcccggcc gaggtgcgta tcctctggaa tctgctcaac atcgaccgct tcgacatcca
     1321 taacctgccc cagtcggtga atgagggcgc gcgcgggctg gccctggccc gtgaattgga
     1381 cctgaaagag gaaacagcct acctgctcaa cgacctcggc gaggcgctgg gcagcctggg
     1441 gcgcatgacc gaagcccacg acatgctggc cgaggccgtg cagcgctggc gcgcgctggg
     1501 caacgagccg atgctcatcg acggcctgag cggccaggcc aactgggcgc tatttaccgg
     1561 cgatctgccg cgcgcccgca cgctggccga cgaggcctat gagcgcagcc tgcgcatcga
     1621 caacccctgg agccaggcct acagcggcgg cgtcagcggc ctgctctatg ccctgcaggg
     1681 cgaaatcggc gcgggccttg atcggctcta ccgctctatc gcccgcgctg aggaggcggg
     1741 cttcgtcggc ggcctgatcc tgatgcgcgc gtttgtctcc aaggcactgc tggcgattgg
     1801 cgctgtggac gaggccgcgg ccgtggccac cgagggcctt gccattggcc gggcgcagct
     1861 gccgcagttc gcgggcatgt gcctggccca gctggcgctg atcagcctgc gcacaggtga
     1921 caccgccacc gccgccgagc tactagagga ccctcttctc caggtggagc atctgcaagc
     1981 ctttgtggag atcgacgcga agctggggtg gattgccctg gccacggcgc aggggcgcac
     2041 cgaagaggct ttgcggcaga tcaccgcgct caaggcccgc gcgcaggaaa tgggcacgat
     2101 cttctggctg ccggaggccc tccaggccga ggccgcggtc ctgctgactc tcgaccgcgc
     2161 cagcgaggcg gccggcgcgc tggccgcggc cgttgacctc agccgccggt cgggcgcgcg
     2221 cttcctgctg tgggtctacc tgtcccagct ggccgacgcg caggataaac tcggcgacgg
     2281 ccgcgccccg gaaacccggg ccgccgccgc cgccgaactc gccaccatca ggcagcacac
     2341 ctggcccgac gcgctgcgcg aatcgctggc gcgttgggcc gcgaccatca gtccccgact
     2401 ataggggcgt gcctgcgtgc gcgctccacg atcccgctgt tcagcagggc tttgcccaag
     2461 tcgtctcgcg accattgacc tgaccggtgg gccgggggtg actttggcaa agcccggccc
     2521 ctttcagcca atccgcccca atctggtaca atacgctcat gccggcgatc agcgacagcg
     2581 cccaactccg tgccgccatg acggccattg aaagccaacg gctggtgctg ggcgacgcgg
     2641 tcgtcgatgc cgccctggtg gccatgcgcg cccaactggt cgcgctcgac gccgcccccg
     2701 cggccgagca acagcgcaaa caggccaccg tcctcttcgc cgatgttgtc ggcttcacca
     2761 cgctggccga aacgaaggac gccgaggagg tcaccgaact gatcaatgcc ctctgggcac
     2821 aactcgacgg gctgatcact gcctacggcg ggcgcatcga caagcacatc ggcgacgcgg
     2881 tcatggccct gtggggcgtc gagtcggccc gcgaggacga cccggagcgc gccatccgcg
     2941 ccgcgctggc catgcaggag gagatcagcg ccttcaacgc cgccatgaag aaccgccgcc
     3001 gcctgcccga tgatctggcc ctggaactgc gcgtcggcat caacaccggc ccggtgctgt
     3061 tggggcaggt gggcctgacg cgcgagtaca cggccatggg cgacacggtc aacctggcca
     3121 accggctgga gcagaccgcg ccgatgggcg gcgtgttggt ggcccacaat accttccgcc
     3181 acgtgcgcgg cgtcttcgat gcccggtcgc tcggccccat ccaggtgcgc ggccggcacg
     3241 aagctgtact cgcctacgtc gtcctgcgcc ccaagccgcg cgccttccgg ctggccacgc
     3301 gcggcgtcga aggcattgag acgcgcacca tcggccgcga cgccgaactg gccgcgctgc
     3361 aagccgcctt cgccgcctcc atcgccgaag cgcgcgcccg catcgtcgct gtcgtcggcg
     3421 aggccggcgt gggcaagagc cgcctgctct acgagttcga caactgggtc gatctgcggc
     3481 cggagcgcgt ccgctacttc aagggccggg ccacgcccgc gctacagggc gtgcccttca
     3541 gcctctggcg cgacgtgttc gcctttcgct tcgacatcct cgacagcgat agcccctctg
     3601 tggccctcaa caagttccgc agcggcttcg tcagccaccg cgccgccgcc gaggccacgg
     3661 ccg
//
LOCUS       DAOSMQ010000322         3649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25453_length_3649_cov_3.674179, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000322 DAOSMQ010000000
VERSION     DAOSMQ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25453_length_3649_cov_3.674179"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..2754)
                     /locus_tag="PK829_10600"
     CDS             complement(19..2754)
                     /locus_tag="PK829_10600"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOU41703.1"
                     /translation="MSAPLSAPQGTGLPRRRLSVVRPRLLAALDQVLASPPDFTQGFA
                     GRLTLVCGPAGFGKTTVVVDWLRRAADRPGLPDVAWLSLEAADNDTARFAASLFAALA
                     LDAAPTMPPLPLLPTLANRLLAGLATRTRPVVLVLDDYYRIEDSAIHEFIAFVIDRLP
                     ATAHLIIISRVDPPLSLGRLRAALEMNELRARDLRFTPAEAEQFLNEFIGLQLNAAEL
                     TLLEQRTEGWIAGLQLAAYALRHETDSTAFLQAFAGNNRYIADYLMEEVLLRQPAERQ
                     SFLLQTSPLARLSSGLCDAVTGRTDSRAMLHSLEAANLFVTAIDPNRQWFRYHQLFAD
                     LLHERLLESGQDVGELHRRAAGWLRDHELLPEAVDHALASGDSSLAVDLMLQAAPELF
                     STSQLSRLTRWAALLPGSALLERPALLLALSWAWVAMGRPVEAEHCVCLYETAIGRTT
                     TELCRLAGTEEIAALDGALLSGLIEAAALWSRLHINKLDIATALQYCRCALRAVDQLT
                     ARPPAPARTAARADTWPLSFNEPPALRPVLLFNMGLALKFVNDTNAAAETLAVAAEAA
                     YQQDNSHLTAIAFGHLAGVQARQGHWRQALDTCGRGLSNVRRLAGKLSPLAGILLAEE
                     GLVRYEVGELSLAEQLWLQAIELAEPWENWEALLPAHVGLIRLRALIRGDSDGARDAL
                     ESLRQLTRHHAAIVSPLIDAYEALLAAPRSGQARWHASPPAGALPYLHEHQQLRELQA
                     MLANRQHWPAVAAAAGQLAAEIEAAGRLGSALHFHLLRAAALERLDQVVGAREALNRA
                     LAIAAPDRVTTPFHEMGLGEHLLGPGDSAPAVGQAGAEKLIDLGADGGLLEMPSERER
                     QVLVLIAEGLSNKEIAARLYLTEGTVKNHAHSLYAKLNVTTRTLAIARARQLGIV"
     gene            complement(2791..3420)
                     /locus_tag="PK829_10605"
     CDS             complement(2791..3420)
                     /locus_tag="PK829_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU41704.1"
                     /translation="MRADFTQARDYALARLASELSPRLSYHSLHHTRDDVLPACLRLA
                     QAGSVADEDVLCLATAAAYHDIGFLYSYAEHEAHSIAMARVVLPGFGYNATQIDAIAA
                     LIAATRMPQQPVTPLAELLCDADLDVLGRDDFWDLNRKLWAETGRYGKNGPVSEEQWL
                     DEQVRFLQGHVYFSAAARRLRDAGKARNVILMQRAIAATRGLDGANGDH"
BASE COUNT          622 a         1159 c         1270 g          598 t
ORIGIN      
        1 ggctacgccg gcaggagcct agacaatgcc cagctggcgc gcgcgggcga tggccagggt
       61 gcgagtggtg acattgagct tggcatagag gctatgggcg tggtttttca ctgttccctc
      121 agtcaggtag agcctggcgg cgatctcctt gttcgacaac ccttcggcaa tcaacaccag
      181 cacctggcgc tcgcgctccg acggcatctc taagagaccg ccgtccgccc ccagatcaat
      241 cagcttctcc gcgccggctt gccccacggc gggagcgctg tcgcctggcc ccagcagatg
      301 ctcgcccagg cccatctcgt ggaagggggt ggttacgcgg tcgggcgcgg cgatggccag
      361 ggcgcggttc agggcctcgc gagcgcccac cacctggtcg agccgctcca gggccgcggc
      421 ccgcaagagg tggaagtgga gagcgctgcc cagacggccg gcggcctcga tctcggccgc
      481 caactggccg gccgcggctg cgacggcggg ccaatgctga cggttggcca acatggcctg
      541 tagctcgcgc aactgctgat gctcatgcag atagggcagc gcgcccgccg gcggcgaggc
      601 atgccaccgt gcctgcccgg atcgcggggc ggccagcagg gcctcgtagg cgtcgatcag
      661 cggcgagacg atggccgcat ggtggcgagt caattggcgc aggctttcca gggcgtcgcg
      721 cgcgccgtcg ctatcgccac gaatgagcgc gcgcagccgt atcaggccga cgtgggcagg
      781 cagcaacgct tcccagttct cccacggctc agccagctcg atggcctgta accatagttg
      841 ctcggccagc gaaagctccc ctacttcata gcgcaccagc ccttcctcgg cgagcagaat
      901 gccggccagt ggcgacagct taccggccag acgccggaca ttgctcagcc ctcgaccgca
      961 ggtgtcgagg gcctgtcgcc agtgtccttg ccgagcctga accccggcca ggtggccaaa
     1021 ggcgatagcg gtgagatggc tgttgtcttg ctggtaggcg gcctccgcgg cgaccgccag
     1081 tgtctcagcg gccgcgttgg tgtcgttgac gaactttagc gccagtccca tattgaacag
     1141 caagaccggg cgtagcgccg gcggctcgtt gaaggatagg ggccaggtat cggcgcgcgc
     1201 ggctgttcgc gcgggagccg gtggccgggc cgtcaactgg tctacggccc gaagcgcgca
     1261 gcgacagtac tgtaatgccg tggcgatgtc gagcttgttg atgtgtagcc gcgaccagag
     1321 cgccgccgcc tcgatcagcc cggagagcag cgcgccgtct aatgcggcga tctcctccgt
     1381 gccggccaga cggcaaagct cagtggtggt ccggccgata gctgtctcgt acaggcagac
     1441 gcagtgttca gcctcgaccg gccggcccat tgccacccag gcccaactca gggccagcaa
     1501 cagcgccggc cgctctagca gagcactgcc gggtagcagg gccgcccagc gcgtcagacg
     1561 ggagagctgg ctggtggaaa atagctccgg cgcggcttgc agcatcaagt ccacggccag
     1621 gctgctatcg ccgctggcca gcgcatggtc aacggcctcc ggcaatagct catggtcgcg
     1681 cagccagccg gccgcgcgcc ggtgcagttc gccgacgtcc tgccctgact cgagcagccg
     1741 ttcgtgcagc aggtcggcaa agagttggtg atagcgaaac cattgccggt tggggtcgat
     1801 ggcggtgacg aagaggttgg ccgcttccag ggagtgcagc attgcccggc tatcagtgcg
     1861 gccggtaacg gcgtcgcaca ggccgctgga aaggcgggcc aggggtgatg tctgcaacag
     1921 gaagctctgc cgctcagccg gctggcgtag caagacctct tccatcaagt agtcggcaat
     1981 gtagcgattg tttccggcga aggcctgcag aaaggccgtg gaatcggtct cgtggcgcaa
     2041 cgcataggcg gccagttgca gcccggcgat ccagccttcg gtgcgctgct ccaggagggt
     2101 aagttcggcg gcgttgagtt gcaaaccaat gaactcgttg agaaattgct ccgcctcggc
     2161 cggcgtgaag cgcagatcgc gggcgcgcag ttcgttcatc tccagagcgg cccgcaatcg
     2221 tcccagtgac agcggcgggt caacccggct gatgatgatc agatgggcgg tcgcgggcag
     2281 gcgatcgatg acgaaggcga tgaactcgtg gatggcgctg tcctcgatgc gatagtagtc
     2341 gtccagcacg aggacaaccg gccgcgtccg tgtggccagc ccggccagga gccggttggc
     2401 cagggtcggc agcaggggca gcggcggcat cgtgggcgct gcgtctagag ccagggccgc
     2461 aaacagcgac gcggcaaagc gggctgtgtc gttgtcggct gcttccagcg acagccaggc
     2521 aacgtcgggc aggccggggc ggtcagcggc acgtcgcagc caatcgacta cgactgtggt
     2581 cttgccgaag ccggccgggc cgcagaccag cgtcagccgg ccggcgaagc cctgggtgaa
     2641 atcaggcggg ctggccagaa cctgatcaag cgccgccagc agacgcggcc gcacgaccga
     2701 cagacgccgg cggggaagtc ctgtgccctg aggcgcggac agcggagcac tcatgggtat
     2761 atcagggtct gcgactgtcg ggcgcggctt tcaatgatcg ccattggccc catccagccc
     2821 tcttgtggcg gcaatggcgc gctgcatcag gatgacgttg cgggccttgc cggcgtcgcg
     2881 caaacggcgg gccgcggccg agaagtagac gtggccttgc agaaagcgca cctgctcatc
     2941 gagccactgc tcttcggaga ccgggccgtt cttgccgtag cggccggtct cggcccagag
     3001 cttgcggttc aagtcccaga agtcgtcgcg ccccagcacg tcgagatcgg cgtcacaaag
     3061 caactcggcc aggggcgtca ccggctgctg gggcattcgg gtggcggcga tgagagcggc
     3121 gatggcgtcg atctgggtcg cgttatagcc gaagccgggc aaaacgacgc gggccatggc
     3181 gatgctgtgg gcctcgtgct cagcatagct gtacaggaag ccgatgtcat ggtaggccgc
     3241 cgcggtcgcc aggcagagca catcctcgtc agcgacgctg ccggcctggg ccagacgcag
     3301 acaggcaggc agcacgtcgt cgcgtgtgtg gtggagcgag tgatagctga gacgcggcga
     3361 tagctcactt gctagtcgag cgagtgcata gtctctggct tgcgtgaaat ctgccctcat
     3421 ttgtctgcta cttgcccacc gctggaatgg cgtaattgta gcgctctctg gctgttgtac
     3481 aatgtggcgc ggttctccga atcgccctct tgcaggcccg cgccacgctc agcagagacc
     3541 attgcaccct gtggcgcgat tctccgaatc gccctcttgc aggcccgcga ttcagagaat
     3601 cgcgccacgc ttggcagaga ccattgcacc ctgtggcgcg gttctccga
//
LOCUS       DAOSMQ010000323         3628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25662_length_3628_cov_4.012874, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000323 DAOSMQ010000000
VERSION     DAOSMQ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25662_length_3628_cov_4.012874"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PK829_10610"
     CDS             <1..1150
                     /locus_tag="PK829_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41705.1"
                     /translation="RWTYSLPGRPLFDPLTALLFYGGLLLALWRWRRPGYALALVWLA
                     VALLPSSFSPDAPSTVRLIGALPVVYLLPGLAVDQIAVWAAMTRRRGGRTAAWALALL
                     MAALLLFNGARTVRDGFVRWPAELETRLRYQTALRDIGRAWATGDGGAPVVAEVFFEP
                     IDADSLRRVIGRDPAARWVQTGGGVSGALVWPAGQTATRLYVPEYAPLDGELAAAAGL
                     GEPLFRSPDAPSFAIYALPARPPDAGTATEESFGQPLAMQLNGFTLLNAKSALTDGQL
                     RLMSTWEVGPALPADVAVFVHLLDAGGAIVAQHDGLDAAATTLRPGDRLVQRHVLLLP
                     ALPPGEYQLVIGLYRRGDGERLPTAGGTGMALLARCAPAGDGVACDLP"
     gene            1229..2485
                     /locus_tag="PK829_10615"
     CDS             1229..2485
                     /locus_tag="PK829_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41706.1"
                     /translation="MPFNHRLHSTWVRHAVVVFAFVLLVLTFVVGMAVAEDGDDTQAA
                     LLIPYAPTTAELLSAPPSIERYVAAHTLPLSARTETGIDRVEASLMSFVPLTTFGLMP
                     QPGQLTLTVGEPNGLNTWPLSWTVDPNATGYELQEAHQLDFSDGQISNVGAVTSLTLT
                     KTPSPENVYYYRVRSRIGDVVGPWSNEGRVVGGFRDNFEDPNSGWALRRTTYIEEVHG
                     RYEDGMYVLDVNDRWDWGISSPGKPAPRLPYVIDFWATVDGGGANLWSFGTVFGGDWP
                     AANCPMSTTADGWYKHGDCFNHFYNTNTIFYGPLKLLFERVDHLEWCLACGGSPLKRL
                     GDINPGSIKTYKLVDPTGWNHYRVEVREDIIKVYAAKLGFEPVLQYTYRDTRYVHNDY
                     FGLFASTDEYTNGIWWFDNFQVMPLD"
     gene            2669..>3628
                     /locus_tag="PK829_10620"
     CDS             2669..>3628
                     /locus_tag="PK829_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41707.1"
                     /translation="MNTKQPVAQPPGRRPRRGLFLAALLALLGVMGLARVGAAEQIDA
                     KPTPPPGAEQTPSPFAALVPTDEELAAMPPGIDRYLAAHTLPASAFQPANLVSSSKTA
                     SQETVTPGGIFDYTVTVRNTGQFDIPVEMTDHLPNQLNYIDSECLAFITDECDYSGGV
                     MTWRGTVPEGGQAVITISVQLKNNAAGGATVVNTAHIESTEQNFDRSATITVDEAESS
                     PLSLLPLTIYGLAPEPGPVTLSVGQPNSANSWTLNWTESVGAHGYDIQEAHQPDFAGA
                     VTIGVGPTTSQAISKSPSPSNVYYYRVRSRVGDLVGPWSNVGQV"
BASE COUNT          596 a         1188 c         1191 g          653 t
ORIGIN      
        1 gcgctggacg tatagcctgc ccggccggcc gctgttcgat ccgctgacgg cgctactatt
       61 ctatggcggc ctgttgttgg cgctgtggcg ttggcggcgg ccgggttatg ccctggcgct
      121 ggtctggctg gctgtggctc tgctgcccag ttctttctcg cccgacgcgc ccagcacagt
      181 gcggctgatc ggcgcgctgc cggtggtcta tctgctgccg gggctggccg tggatcagat
      241 cgccgtgtgg gcagccatga cccgtcgccg cggcgggcga accgcggcct gggcgctggc
      301 gctgttgatg gccgcgctgc tgctgttcaa cggtgcccgc accgtgcgcg atggcttcgt
      361 gcgctggccg gcggagttgg agacgcggtt gcgctatcag accgcgctgc gcgacatcgg
      421 ccgggcctgg gcgactggcg atggcggcgc gccggtcgtg gccgaggtct tctttgagcc
      481 aatcgacgcc gacagcctgc gccgcgtcat tggccgcgac ccggcggcgc gctgggtgca
      541 gaccggcggc ggggtgtccg gagcgctggt gtggccggcc gggcagacgg ccacgcgcct
      601 ctacgtgccc gaatatgcgc cgctggatgg cgaattggcc gccgccgccg gcctgggcga
      661 gccgcttttc cgcagccccg acgcgccctc attcgccatc tatgccctgc cggcgcgtcc
      721 gccggacgct gggacggcca ccgaggagag cttcggccag ccgctggcca tgcaactgaa
      781 cggctttacc ttgctgaacg ccaagagcgc gctgactgac gggcagctac ggctgatgtc
      841 aacgtgggag gtggggccgg cgctgccggc cgatgtggcc gtgttcgttc acctgttgga
      901 cgccggcggg gccatcgtcg cccaacacga tgggctggac gcggcggcga cgacgttgcg
      961 acccggcgac cgtctggtgc agcggcacgt tctattgctg ccggcgctgc cgccgggcga
     1021 gtaccagctg gtgatcggtc tgtaccggcg cggcgacggc gagcggctgc ccacggctgg
     1081 cggcacgggg atggcgctgc tggcccgttg cgcgccggca ggcgatggcg tggcctgtga
     1141 tttgccatag caggggcaaa tggtagcatt ccaactggat ataggggtta tgtccagaac
     1201 gcggtttact tgacaggaga gaatccacat gccgttcaat caccgactgc attcaacctg
     1261 ggtcaggcat gctgtcgtcg ttttcgcctt cgtcttgctc gtcctgacat tcgtcgtcgg
     1321 gatggctgta gccgaggatg gcgacgatac gcaagccgca ctattgatcc cctatgcgcc
     1381 gacgacggcc gaattgctgt ccgcgccgcc atcgatcgag cgctacgtgg ccgcgcacac
     1441 gctgccactc tcggcccgca ctgagacggg tatcgaccgt gtcgaggcct ccctgatgag
     1501 cttcgtgcct ctaacgacgt tcggcctgat gcctcaaccg gggcagctca ccctgacggt
     1561 gggcgagccg aatggcttaa acacctggcc gctgtcctgg acggttgacc ccaacgccac
     1621 cggctacgag ctgcaagagg ctcatcaact cgatttcagt gatggccaaa tcagcaacgt
     1681 gggcgcggtc acgagcctga ccctgaccaa gacgccctca ccggagaacg tctactacta
     1741 ccgcgtccgc agccgcatcg gcgacgtcgt ggggccgtgg tcaaacgagg gccgcgtcgt
     1801 tggtggcttc cgggataact ttgaggaccc caacagcggc tgggctttgc gccggacgac
     1861 ctacatcgag gaagtgcacg gtcgctacga ggatggcatg tacgtcctgg atgtcaacga
     1921 ccgctgggac tggggcatct ccagccccgg caagcccgcc ccgcgcctgc cctacgtgat
     1981 cgacttctgg gctaccgttg acggcggcgg agcaaacttg tggtcgttcg ggacggtctt
     2041 cggtggcgac tggccggcgg ccaactgccc gatgagcacc actgctgacg gttggtacaa
     2101 gcacggtgac tgcttcaacc acttctataa taccaatacc atcttctatg gcccgctcaa
     2161 gctgttgttc gagcgcgtcg accatctgga gtggtgcctg gcgtgcggcg gcagcccgct
     2221 caagcgcctc ggcgacatca accccggcag catcaagacc tacaagctag tcgatccgac
     2281 cggctggaac cactatcgtg tcgaagtgag agaggacatc atcaaggtct acgcggccaa
     2341 gctgggcttc gagccggtgc tgcaatacac ctatcgcgat acccgctacg tccacaacga
     2401 ttactttggt ctattcgcct cgaccgacga gtacaccaat ggcatctggt ggttcgacaa
     2461 cttccaggta atgcctctcg actagggcgc ggggcaaatc cattcgctga cgggccaacg
     2521 gcgggcgatg cgcttgccgc tggcccgttt gcgtgggggc ggcgtagggg cggcgtaggg
     2581 cgcgtccgtt tgcccggatg gctggtgggc agtaccatag ccgctattca gcggttcact
     2641 gaaccggtat aggaaggagc aaagagccat gaatacgaag caacctgttg cccagccgcc
     2701 cggccggaga ccgcgccgcg gccttttcct ggcggctctg ttggcgcttc tgggtgtcat
     2761 gggcctggcc cgcgtgggcg cggccgagca gatcgacgcc aagcccacgc cgccccccgg
     2821 cgccgagcaa acgccctcgc ccttcgcggc attggtaccg acggacgagg aattggccgc
     2881 tatgccaccg ggcatcgacc gctacctggc cgcccacacg ctgccggcca gcgcctttca
     2941 gccagccaac ctggtcagtt catccaagac cgcctcgcag gagacggtta cccccggtgg
     3001 catcttcgac tacacggtca ccgtccgtaa cacgggccag ttcgatatcc ccgtggagat
     3061 gaccgaccac ctgcctaacc aactaaacta catcgatagc gagtgcctgg cctttatcac
     3121 tgacgaatgc gactacagcg gcggcgtgat gacctggcgc ggaactgtgc cggagggagg
     3181 acaggcggtg atcaccatct ccgtccaact gaagaacaac gccgccggcg gcgctacggt
     3241 cgtcaacacg gcccacatcg aaagcacgga gcagaacttt gaccgcagcg cgaccatcac
     3301 cgttgatgag gccgagtcgt cgccgctcag tttgttgccg ttgaccatct atggcctggc
     3361 tcccgaaccc ggcccggtca cgctgagtgt gggccagccc aacagcgcca acagttggac
     3421 gctcaactgg acggagagcg tcggcgcgca tggctatgac atccaggaag cccaccagcc
     3481 agacttcgcc ggtgccgtta ccatcggcgt cggcccgacg acttcgcagg cgatcagcaa
     3541 gtcgcccagt ccttctaacg tctactacta tcgtgtgcgc agccgcgtcg gcgatctggt
     3601 cgggccgtgg tcgaatgtgg gccaggtc
//
LOCUS       DAOSMQ010000324         3626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25679_length_3626_cov_5.530944, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000324 DAOSMQ010000000
VERSION     DAOSMQ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25679_length_3626_cov_5.530944"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PK829_10625"
     CDS             complement(<1..718)
                     /locus_tag="PK829_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU41708.1"
                     /translation="MTTDVVTDVGARTNGDAPPANGVHNPYGSPPGSGVTLPPGYQPT
                     PSVKNKNNYFPQSEPLGPDEMRIIFMGSQPWPPRQTQAGTCIMVELGNGKRFFFDFGP
                     GCLRNIIANQVPVPEINDIFLTHLHLDHYADIPYLWQFAPFNGRWKPLRIHGPSSSRP
                     ELGTRAMVDHMAQMGLWTSIQAEGMPLHDGYEFEVNEFDFRDDGGVCYDKDGVVITHW
                     RRSHAADGASAYRLDWNGLSF"
     gene            complement(777..1445)
                     /locus_tag="PK829_10630"
     CDS             complement(777..1445)
                     /locus_tag="PK829_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41709.1"
                     /translation="MKAAIAHPATTPNAAAAAGIAFAVLFGLGLTLVRLAADALGAGE
                     ALSEETRGRLSLALALAPFAGIFFLWFIGVVRHHLGAYEDQFFATVSLGSGLLFVALT
                     FVAFAVAGGTLAAYAGGPLAAGTYALNRAIIDQLFNTYALKMASVFMLSLGTLWRKTG
                     VMPRSLTLVTYALALVMMVTLSHNLWLSLIFPVWVLLVSVYILFAGLHHRAEGSAVFQ
                     PQQS"
     gene            complement(1483..1818)
                     /locus_tag="PK829_10635"
     CDS             complement(1483..1818)
                     /locus_tag="PK829_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41710.1"
                     /translation="MKRRVILFGGTVILGALGASLAPYPELEVIALVPPLPGARELGA
                     LAPDVILFDAGSAQPGPAFALLRKRPNLTLIGVDPEGDRITVWSCGQTNAASAEELLR
                     VITQALGRL"
     gene            complement(1957..2601)
                     /locus_tag="PK829_10640"
     CDS             complement(1957..2601)
                     /locus_tag="PK829_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU41711.1"
                     /translation="MNVLLVDDHHLLLEGLQNLLAAHGVQVVGLAHDGLEAVELARAT
                     RPDVILMDIRMPRGDGLEATRRICAEWPAARIVILTTSTEDADLFEAVKSGACGYLLK
                     SMNADELIECLHGAQEGVPPFSPGLAAKVLGEYARLAGQRPAPVGEAEAELTPRQREV
                     LALVAEGLTYKEVGARLNLSPRTIKYHMAAIMAQLHLEHRAQVLAYAGRLGLDS"
     gene            complement(2598..>3626)
                     /locus_tag="PK829_10645"
     CDS             complement(2598..>3626)
                     /locus_tag="PK829_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU41712.1"
                     /translation="QSAIDQLDAGVLVLDRTGRVVGHNPAAANMLGLAGEEIRGRPIS
                     ALLPAGTAETIHGAMAAGSSGFHDTSLRERDIQLQTSPLRDWRGLDVGRLLLLHDVTA
                     QNQAQTQLLAQQRVLATLQERERLARELHDSAAQILSYTSIQAQTIQKHVHDGNLATA
                     EAQLARLAEVASAAHTDVRESILGLTASGGAAPRFLSSLGQFLAVYKTNYGIATELAV
                     AEDLGEDDFAPDTTVQLLRVIAEALTNARKHAHAGRICVDVARADGVARVDGSARVDG
                     GARITIADDGRGFDIGLAGALEHYGLAFMRERMAQVGGRLAIDSRPAAGTWVVLDVPL
                     PMRQEVTT"
BASE COUNT          611 a         1175 c         1249 g          591 t
ORIGIN      
        1 cgaacgacag gccgttccag tcgaggcgat aggccgaagc gccatcggcc gcgtgtgagc
       61 gccgccagtg ggtgatgacc acgccatcct tgtcgtagca cacgccgccg tcgtcgcgga
      121 agtcgaactc gttgacctcg aactcatagc cgtcgtgcag cggcatgccc tcggcctgga
      181 tggatgtcca cagccccatc tgggccatgt gatcgaccat cgcccgcgta cccaactccg
      241 gccggctgct cgacgggccg tggatgcgca gcggcttcca gcgcccattg aacggcgcga
      301 actgccacag gtaggggatg tcggcgtagt ggtcgaggtg caggtgggtg aggaagatgt
      361 cgttgatctc cggcacaggc acctggttgg caatgatatt gcgcagacag ccggggccga
      421 agtcgaagaa gaagcgcttg ccgttgccca actcgaccat gatgcacgtg cccgcctgcg
      481 tctggcgcgg cggccacggc tggctgccca tgaagatgat gcgcatctca tccggcccca
      541 gcggctcgct ctgcggaaag tagttgttct tgttcttgac ggacggcgtc ggctggtatc
      601 ccggcggcag ggtcacgccg ctgcctggcg gcgagccgta ggggttatga acaccattgg
      661 cgggcggcgc gtcgccgttc gtccgcgcgc ccacgtcggt cacaacatcg gttgtcattg
      721 gttgatctcc ttgtcactgt tcaaggcgac gggcggccgg ccgccgcgtc gtcgatctat
      781 gattgttggg gttggaagac ggcgctgccc tcggcccgat ggtgcaggcc ggcgaagagg
      841 atgtagacgc tgaccagcag cacccagacc gggaagatca gcgatagcca caggttgtgg
      901 ctgagggtga ccatcatcac cagcgccaga gcgtaggtaa ccagcgtcag actgcgcggc
      961 atcacgccgg tcttgcgcca gagcgtgcct agcgagagca tgaagacgct ggccatcttc
     1021 agggcgtaag tgttgaagag ctggtcgatg atggcccggt tgagcgcgta ggttccggcc
     1081 gccagcggcc cgcccgcata ggcggccagc gtgccccccg ccacggcaaa ggcaacgaag
     1141 gtcagggcga cgaacagcaa cccgctgccc aggctgaccg tggcgaagaa ctggtcttcg
     1201 taggctccca gatggtggcg gacgacgccg atgaaccaca ggaagaagat gccggcgaag
     1261 ggcgccaggg ccagggccag cgacagccgg ccgcgcgtct cttcgctcaa cgcctcgccg
     1321 gcgcccagcg cgtcggccgc caggcgaacg agcgtcaggc caagcccgaa gagcacggca
     1381 aaggcgatgc cggcggcggc ggcggcgttg ggcgtcgtgg ccgggtgggc gatggcggct
     1441 ttcatggatg atgtcatgat ggcaaatacc tccctggccg gcctagagcc ggccgagcgc
     1501 ctgagtgatg acccgcaaca gttcctcggc cgacgcggcg ttggtctggc cgcacgacca
     1561 gactgtgatt cggtcgcctt ctggatccac accaatgagc gtcaggttgg ggcgcttgcg
     1621 caacaatgcg aaggcggggc cgggttgggc gctcccggcg tcgaacagga tgacgtccgg
     1681 cgcgagcgcg cccagttccc tggctcccgg cagaggtgga acgagcgcga tgacttctag
     1741 ttcggggtag ggggcgaggc tggcccccaa cgcgccgagg atgaccgtgc caccgaacag
     1801 aatcacccgc cgtttcacag tattcttctc ctgactacca gagtagcagg tcaacggggc
     1861 cggccacaat gggccgcggg acagaaaagg gacagtcctc cgggcgagag ggctgtccca
     1921 aaggacagtc agacgcagcg ttttgctcgg ttgaggctac gagtccagtc ctagccggcc
     1981 ggcataggcc aatacctggg cgcggtgttc caggtgtagc tgagccatga tcgcggccat
     2041 gtggtacttg atcgtccgcg ggctgaggtt caggcgcgcg ccaacctcct tataggtcaa
     2101 cccttcggcc accagagcca atacctcgcg ctggcggggg gtcagttcag cctcagcctc
     2161 cccgaccggc gcgggccgct gcccggccag gcgcgcgtac tcgcctagca ctttggcggc
     2221 cagaccaggc gagaagggcg gcacgccctc ctgcgccccg tgcaggcact cgatcagctc
     2281 gtcggcgttc atgctcttga gcagatagcc gcacgcgcca ctcttgacgg cctcgaacag
     2341 gtcggcgtcc tcggtcgacg tcgtcaggat gacaatcctg gccgcgggcc actcggcgca
     2401 gatgcggcgc gtggcctcca gcccatcgcc gcgaggcatg cgaatgtcca tcaggatgac
     2461 gtccggccgc gtcgcgcggg ccagttcaac cgcttccagc ccatcgtggg ccagtccgac
     2521 gacctgcacg ccgtgggccg ccagcaagtt ttgcagccct tccagcagta agtggtgatc
     2581 atcgaccagg agaacgttca tgtggttacc tcttgccgca taggcagggg cacgtccagc
     2641 acgacccatg tgcccgcggc ggggcggctg tcaatggcca gccggccgcc gacctgggcc
     2701 atgcgctcgc gcatgaacgc caggccatag tgttcgagcg cgccagccag gccgatgtca
     2761 aacccgcggc cgtcgtcagc gatagtgatg cgggcgccgc cgtccacgcg ggcactgccg
     2821 tccacgcggg ccacgccgtc cgcgcgggcc acgtcaacac agatgcggcc agcgtgggca
     2881 tgcttgcggg cattggtcag cgcctcggcg atcacgcgca gcagctggac ggtggtatcc
     2941 ggcgcgaagt catcctctcc caggtcttcg gctacggcca gttccgtggc aatgccatag
     3001 ttggttttat agacggccag gaattggccc aaagacgaca ggaaccgcgg cgcggccccg
     3061 ccgctggcgg tgaggcccag gatcgattcg cgcacgtcgg tatgggcagc cgaggccacc
     3121 tcggccaggc gcgccagttg ggcctcggcc gtggccagat tgccgtcgtg aacgtgcttc
     3181 tgaatggttt gggcctggat gctggtgtag ctgagtatct gcgcggcgct atcgtgcagc
     3241 tcccgcgcca accgctcacg ctcttgcagc gtggccagaa cgcgctgctg cgccagaagc
     3301 tgggtctgcg cctggttttg cgcggtgacg tcatggagca gcagcaggcg gcccacgtcc
     3361 agcccgcgcc agtctcgcag gggcgaggtc tgcaactgga tatcgcgctc gcgcagggac
     3421 gtgtcgtgga aaccgctgct gccggcggcc attgcgccgt ggatggtctc ggccgtgccg
     3481 gcgggcaaca gtgcgctgat tggacgcccg cgaatctctt ccccagcaag ccctagcatg
     3541 ttcgccgcgg ccgggttgtg gccgaccacg cgaccggtgc gatcgagcac cagcacgccg
     3601 gcgtccagtt ggtcgatggc cgactg
//
LOCUS       DAOSMQ010000325         3608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25863_length_3608_cov_4.737124, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000325 DAOSMQ010000000
VERSION     DAOSMQ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25863_length_3608_cov_4.737124"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1458)
                     /locus_tag="PK829_10650"
     CDS             complement(166..1458)
                     /locus_tag="PK829_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU41713.1"
                     /translation="MTSQIFFEIALLLFLFLLNGIFAMAELAIVSSRRERLQLLAESH
                     PGARAALELAADPSNLLSTVQVGITLIGILAGAFGGATLTAELTKLLDGLPVVGPYAG
                     AIALVVVVAAITFGSVVLGELAPKQLALRSPERLAATLARPMNVLLRFTRPVVRLLNG
                     TTALLLRALGVRVNGGEQQVTEQEIQMMIDQGAAAGVFEAAERDMLTSIFRFGDRQLR
                     SLMTPRTEVVWLDVNDDEATIRDTVLHSHHSRFPLCDGSIDRVIGVVLARDLLEKTWT
                     GQPLDLRALARQPLFLPETMLALRALERFQETGIQAALLVDEFGGVEGVVTIMDMMAS
                     IVGDIPTVSDIADPPVVLREDGSWLVDGQLDTDDLKRLLAVTQLPDAAGYQTLGGFLV
                     TRLGRLPRAGDVVHWGGYRFEIVDMDGNRVDKVLVQPA"
     gene            1561..>3608
                     /locus_tag="PK829_10655"
     CDS             1561..>3608
                     /locus_tag="PK829_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU41714.1"
                     /translation="MDLLHNLNPAQRDAVSAPPGPLLVVAGPGSGKTRVLTHRIAYLI
                     QDMGVSPWHIMAVTFTNKAAREMEHRIEQLLDGRPQGLHMGTFHAICARLLRREADNL
                     THYQRDFVIFDTADQLQVVKSALAELRLDDKKFPPAKMLNGISTAKNDLITPEAYQAT
                     NYIGEITRRVYEYYQLALQTNNAMDFDDLLMQAVLLFDERPDVLQKYQRRYHHILVDE
                     FQDTNATQYRLLQQLAGDDNSIFAVGDSDQSIYKWRGADYRNVQNFYRDYPTARTIML
                     EQNYRSTQVILDAAIGVIQRNADHMPKKLFTERKGGAKLIVREAYNETDEANAVVDTI
                     QDLMLQGYSGNACAAMYRTNAQSRALEEAFIGAGMPYRLVGATQFYQRREIKDVIAYL
                     RVVHNPRDAVSLGRILNVPTRGIGQATQQQFYLWAAGLGLQPAEALVRLATDADLQHP
                     FTGRAYNALHTFGGQLANWIALGERVAVGDLLDAILRDTRYQAHLDDGTDEGVDRWEN
                     VMELRGVAGSDESLSLGDFLQQVALVAETDNLDNAAQATTLLTLHAAKGLEFPIVFIT
                     GLEEGLLPHSRSMDSEDELAEERRLFYVGMTRAKDRLYLSHVFRRSTWGDSSVAVPSR
                     FLNDIPEELLEGGGAGQRRRQSIDRASTWSDTRPRTGFDRRPASSPPPAPRPTTPAFG
                     "
BASE COUNT          686 a         1264 c         1112 g          546 t
ORIGIN      
        1 ctgttttggc cttgagaggg ccacgtcctg ggccgctaga cgaatggggc caacggtggg
       61 gattctagca ctgccggcag ggcctgtcaa acggcttcgg ccggcccaca gagcttttct
      121 aaagtcgtct agcaaccata ttgacctggg ccagcccaca agcggctagg ccggctggac
      181 caagaccttg tctacccggt tgccatccat atccacgatc tcgaagcgat agccgcccca
      241 gtgtaccacg tcgccggcgc gcggcaggcg gccgagccgc gtcacgagga agccacccag
      301 cgtttggtag ccggccgcgt cgggcaattg cgttacagcc agcaggcgct tcaggtcatc
      361 cgtatccagt tggccatcga ccagccaaga gccgtcctcg cgcagaacaa cgggtgggtc
      421 ggcaatgtcg ctcactgtag gaatgtcgcc aacaatggac gccatcatgt ccataatcgt
      481 caccaccccc tcgacgccgc cgaactcatc gaccagcagc gccgcctgga tgcccgtctc
      541 ctggaagcgc tccagcgccc gcagagccag catcgtctcc ggcaggaaga gcggctgccg
      601 cgccagggcg cgcaagtcca gtggctgccc cgtccacgtc ttttccaaca ggtcgcgggc
      661 cagcaccacg ccgatgaccc ggtcgatgga gccgtcgcac agggggaagc gggaatggtg
      721 ggaatggaga acggtgtcgc ggatggtggc ctcgtcgtcg ttgacgtcga gccagacgac
      781 ctcagtccgc ggcgtcatca gcgagcgcaa ctgccggtcg ccaaagcgga agatgctggt
      841 cagcatgtcg cgctcggccg cctcgaagac gccggccgcc gctccctggt cgatcatcat
      901 ctgaatttcc tgctccgtga cctgctgctc ccctccattg acgcgcaccc ccagagcgcg
      961 caacagcagc gccgtcgtcc cattgagcaa gcgtaccacc ggccgcgtga accgcagaag
     1021 cacattcatc ggccgcgcca gggtggcggc cagccgctcc gggctgcgca gcgccagctg
     1081 cttgggggcc agctcgccca ggacgacgct gccgaaggtg atggccgcga caaccaccac
     1141 cagggcgatg gctcccgcat aggggccgac gaccggcagc ccgtcgagca gctttgtcag
     1201 ctcggccgtc agcgttgccc cgccgaacgc gccggccagg atgccgatca gagtgatgcc
     1261 cacctgcacg gtcgagagca ggttcgacgg gtcggcggcc agttccaggg ccgcgcgcgc
     1321 gccggggtgg ctctcggcca gcagttgcag ccgctcgcgc cgcgaggaga cgatagccag
     1381 ttcggccatg gcgaagatgc cgttgagcag gaataggaag agaagaaggg cgatctcgaa
     1441 gaaaatttgt gaagtcatgc gctcccaacg agtgaatggc gacaaagtct catgcctgac
     1501 agtataaacc atgcccggcg ggaattggga agagttggcc ggaaggacta aaatacgggt
     1561 atggatttgc tgcacaacct caaccccgcc cagcgggatg ccgtcagcgc cccgcccggt
     1621 ccgctgctgg tcgtcgccgg ccccggcagc ggcaagaccc gcgtcctgac ccaccgcatc
     1681 gcctatctca ttcaggacat gggcgtctcg ccgtggcaca tcatggccgt gaccttcacc
     1741 aacaaggctg cccgcgagat ggagcaccgc atcgagcaat tgctggacgg ccggccgcag
     1801 ggcctccaca tgggcacctt ccatgccatc tgtgcccgcc tgttgcgccg cgaggccgac
     1861 aacctgaccc attaccagcg cgatttcgtc atcttcgaca cggccgacca gctccaggtt
     1921 gtcaagtccg ctctggccga gctgcggctg gacgacaaga agttcccgcc ggccaagatg
     1981 ctcaacggca tcagcaccgc caagaacgac ctgatcacgc cggaggccta ccaggcgacc
     2041 aactatatcg gcgagatcac ccggcgcgtc tacgagtact accagctggc cttgcagacc
     2101 aacaacgcca tggacttcga cgacctgctc atgcaggcgg tgctgctgtt cgacgagcgg
     2161 ccggacgtgc tccagaagta ccagcgtcgc tatcaccaca ttctcgtgga cgagttccag
     2221 gacaccaacg ccactcagta ccgtctgtta cagcagttgg ccggcgacga caacagcatc
     2281 ttcgctgtgg gcgactccga ccaatcgatc tacaagtggc gcggcgcgga ttaccgcaac
     2341 gtccagaact tctaccgcga ctaccccacg gcgcggacga tcatgctgga gcagaactac
     2401 cgctcgacgc aggtcattct cgacgcggcc ataggcgtca tccagcgcaa cgccgaccac
     2461 atgcccaaga agctcttcac cgagcgcaaa ggcggggcca agctcatcgt gcgcgaggcc
     2521 tacaatgaga ccgacgaggc caacgccgtc gtcgatacca tccaggacct catgctgcaa
     2581 ggctacagcg gcaacgcctg cgcggccatg tatcgcacca acgcccaatc ccgcgcgctg
     2641 gaagaggcgt tcatcggcgc gggcatgccc tatcgcctgg tcggggcgac gcagttctat
     2701 caacgacgcg agatcaagga cgttatcgcc tatctgcgcg tcgtccacaa cccgcgcgac
     2761 gccgtcagcc tgggccgcat cctcaacgtg cccacgcgcg gcatcggcca ggcgacacaa
     2821 cagcagttct atctctgggc ggccgggctg gggctgcaac cggccgaggc gctggtgcgg
     2881 ctggccaccg acgccgacct gcaacatccc ttcaccggcc gggcctacaa tgccctccat
     2941 accttcggtg gtcaactggc caactggatc gctcttggtg agcgcgtcgc cgtgggcgat
     3001 ctgctcgacg ctatcctgcg cgacacgcgc taccaggccc acctcgacga cggcaccgac
     3061 gaaggcgttg accgctggga gaacgtcatg gagttgcgcg gcgtggccgg cagcgacgaa
     3121 tcgctcagcc tgggcgactt cttgcagcag gtcgccctgg tggccgagac ggacaacctc
     3181 gacaacgccg cccaggcgac gacgctgctg accctccatg ccgccaaggg actggagttt
     3241 cccatcgtct tcatcaccgg gctggaggag gggctactgc cccacagccg ctcgatggac
     3301 agcgaggacg aactggccga agagcgccgc ctgttctacg tcggcatgac gcgggccaag
     3361 gaccggctgt acctgtcgca cgtcttccgc cgctccacct ggggcgacag ctccgtcgcc
     3421 gtgccgtcgc gcttcctcaa cgacattccg gaggaactgc tggaaggcgg cggggccggc
     3481 cagcgccgcc ggcagagcat cgaccgggcc agcacgtgga gcgacacgcg cccgcgcacc
     3541 ggcttcgacc gccgcccggc cagcagcccg ccgcccgcgc cgcggccaac cacgcccgcc
     3601 tttggctg
//
LOCUS       DAOSMQ010000326         3603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25903_length_3603_cov_4.053269, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000326 DAOSMQ010000000
VERSION     DAOSMQ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25903_length_3603_cov_4.053269"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PK829_10660"
     CDS             <1..1023
                     /locus_tag="PK829_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41715.1"
                     /translation="GAGRPSAAIAYDEPIHPDFQTEAAQSPRPARRSWGVEPRAVLLG
                     LLLLAMLALAGWAVLTFLGGRGQAGSQGAIVTLPTQAATVDMSVGQTVSAVAVGQAAE
                     ATATALGVAITPTVATAREATAAATEVDSVAATATALFAGVVATAECRAPDWWVAYVT
                     QEGDTVEALSAARGILPEELIVANCLASPELTAGMALRLPPVGVIVLLPGATVTPGAT
                     ATPATTARPNLPTPTFPAIFPTRPLLLLPTPTFPVLVVTTPELPEPEPGEPARPPRPT
                     SAPPPPNATSTPPIFATSTPPIFGPTATPPIFGTAQATKTPPGDQEATPTVTGTIPAT
                     RTPPAP"
     gene            1071..3065
                     /locus_tag="PK829_10665"
     CDS             1071..3065
                     /locus_tag="PK829_10665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU41716.1"
                     /translation="MDKPLANRLESRAVFLRTSAVDVVVLALTAPFLLAAGWAPRSLS
                     LAALALLAVPFVVRRGRTGRFARPMALNWPVTVLGLVFLPAAMLLSPAPWAVSWPRAA
                     TLAWSIVLFYAVANWPARPRVHRHGRQATRLSGPTWGFLALGGVVAVLAPLVMRSVDK
                     LFFLPQTGWLATRLGWQHALPTNEVAGVLTLFIPFTVALAAAAWWTQRRRLLAALAPL
                     TLLLLAALALTQSRTGLMASAVGALLALLLGARPRRKTLVIGLGAVALILAAVALSPL
                     REWFVFAGANSWQSVIGPRLGIWGQALDGIRDHPVWGMGLGAFGLLARLIYPLVPVAQ
                     SAVIEDAHNLYLQTTLDFGILGGLLLLLLLGVAVWSGWGLARARPPRALSRLWAAGLV
                     GALVAHALYSLTDAVALGALGGVALWYVFGLIMSSTTTRTERAVEPFQRWSPLVIGVA
                     GLMLLSALMMTARPVNRAGQLAGAVALNTSQSAATAAATAGPLTAARCRAGWYEGLLW
                     HAAGDMARRSAVWAELLACATDYTGYMAVLAGDDAALARRAVDVQPDNPAGYFWLAAL
                     SAPDEAIGLYRQGLALAPGDGRRWLALAELLDGRDEDAALDAYLQACLHGDPGANGCA
                     RAAAIVAARGDLETAIRYYRLSNWSEARAMADELERQLDP"
     gene            3147..>3603
                     /locus_tag="PK829_10670"
     CDS             3147..>3603
                     /locus_tag="PK829_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU41717.1"
                     /translation="MSILNASRVGQSFGEFDVFSGVSVSIPRDGKIGLVGPNGVGKTT
                     LLLILAGRAQPSSGAVHVARGTRIGYLSQESSDAFVGAMAEQTVYDEMLTVFAELRAV
                     EERLRVLEHEMGAADAHDALLDEYSQLQAQYEVGGGYEYDTRVRMVLTGL"
BASE COUNT          453 a         1284 c         1260 g          606 t
ORIGIN      
        1 ggcgcgggtc gcccctcggc ggcgatcgcg tacgacgagc cgatccaccc cgactttcag
       61 accgaagcgg cgcagtcgcc gcggccggcg cgccgttcct ggggcgttga gccgcgcgcc
      121 gtgttgctgg gcctcttgct gctggcgatg ctggcgctgg ccggctgggc cgtactcacc
      181 tttctgggtg ggcgcggcca ggccggctct cagggcgcta tcgtgaccct gccgacgcag
      241 gcggcgacgg tcgatatgag cgtgggacag acagtcagcg ccgttgccgt gggccaggcc
      301 gccgaagcca cggctaccgc gctgggcgta gccatcacgc cgactgtggc gaccgccagg
      361 gaagccacag ccgccgccac cgaagtggac agcgtggccg ccacggccac ggcgctcttt
      421 gccggggttg tggctacggc cgagtgtcgc gcgccggatt ggtgggttgc ctacgtcacc
      481 caggaaggcg acacggttga ggcgctgtcc gcggcccgcg gcatcctgcc ggaggaactc
      541 attgtcgcca attgcctggc ctccccggaa ctgacggccg gcatggcctt gcggctgccg
      601 ccggtgggcg tcatcgtcct gttgccgggg gctaccgtca cgccaggggc caccgccaca
      661 ccggcgacca ccgcccggcc caacttgccc acgccaactt tcccggcgat ctttccgacc
      721 cggccactgc tgttgctgcc gacgcccacg tttccggtgc ttgtcgtgac tacgccggag
      781 ttgccggagc cggagccggg cgagccggcg cgcccgccgc gcccgacgtc cgcgccgccg
      841 ccaccaaacg cgacctcgac gccacccatc ttcgcaacct caacgccgcc tatctttggg
      901 ccaacggcga cgccgcctat cttcggcacc gcccaggcca ccaagacacc gccaggcgac
      961 caggaggcca cgccgacggt cacaggcacg atcccggcca cacggacgcc gcccgcccct
     1021 tagcccggcc caccgggcgc tatttggccc tggcattgct tgaggagtct gtggataagc
     1081 cactcgccaa ccgccttgag tcgcgcgccg tcttcttgag aacgagcgcg gtcgatgtcg
     1141 tcgtgttggc gttgaccgcg ccattcctgt tggcagcggg gtgggcgccg cggtcgttgt
     1201 cgctggccgc gctggcgctg ctggccgtgc cgtttgtcgt ccggcgcggg cgcacggggc
     1261 gctttgcccg gccgatggcc ctcaactggc cggtgacggt gctgggcctg gtgtttctgc
     1321 ccgcggccat gctgctcagc cccgcgccct gggcagttag ttggccccgc gcggctacat
     1381 tggcctggtc tattgtcttg ttctacgccg ttgccaattg gccggcccgg ccgcgcgtcc
     1441 atcgccacgg tcgccaagcc acgcggctaa gcgggccgac atggggcttt ctggcgctgg
     1501 gtggcgttgt cgctgtgctg gcccccctgg tcatgcgcag tgtggacaag ctcttctttc
     1561 tgccgcagac gggctggctg gcaacccggc tcggctggca gcacgccctg ccgaccaacg
     1621 aggtcgccgg ggtgctgacg ctgttcatcc cctttaccgt ggcactggcc gccgcggcct
     1681 ggtggacgca gcgacggcgg ctgttggcgg ctctcgcgcc actgacgctg ctcttgctgg
     1741 cggcgctggc gctaacccag tcgcgcaccg gtctgatggc cagcgcggtc ggcgctctgc
     1801 tggccctgct tctcggcgcg cgcccgcgcc ggaagacact ggtcatcggc ctgggcgcgg
     1861 tggcgctcat cctggcggcc gtcgcgctgt cgccgttgcg cgagtggttt gtcttcgccg
     1921 gggccaactc ctggcagagc gtcatcgggc cgcgcctggg catctggggc caggcgctag
     1981 acggcatccg cgatcatccc gtgtggggca tggggctggg cgcgttcggc ttgctggccc
     2041 ggctgatcta cccgctcgtt ccggttgccc aatcggccgt catcgaagac gcccacaatc
     2101 tgtacctcca gaccacgctc gatttcggca tcctgggcgg gttgttgctg ctgttgttgc
     2161 tgggggtggc ggtgtggtcg ggctgggggt tggcccgcgc gcgcccgccc cgcgctctat
     2221 cgcggctgtg ggccgccggg ctggtgggcg cgctggtggc ccatgcgctc tacagcctga
     2281 ccgacgcggt agccctgggc gcgctgggtg gcgtcgccct ttggtacgtg ttcgggctga
     2341 tcatgagcag cacaaccacg cgcacggagc gagcggtcga gccattccaa cgctggtcgc
     2401 ccctggttat cggggtggcc gggttgatgc tgctgtctgc gctgatgatg accgcgcggc
     2461 ctgtcaaccg cgccggccaa ctggccggcg cagtagcgct gaacacgtcc cagagcgccg
     2521 cgacagcggc ggcgacggcc gggccgctga ccgcggcccg ctgccgcgcg ggctggtatg
     2581 agggcctgtt gtggcacgcc gccggcgaca tggcgcggcg ctcggccgtc tgggccgaac
     2641 tgctggcctg cgccacagac tacaccggct atatggctgt gttggccggc gatgacgcgg
     2701 ccctggcccg gcgggccgtc gacgtgcaac cagacaatcc ggcaggctac ttctggctgg
     2761 cggccctgtc cgcgcccgac gaagccatcg gtctttaccg gcagggcctg gccctggccc
     2821 ccggcgacgg gcggcgctgg ctggcgctgg ccgaactgct cgacggccgg gacgaagacg
     2881 ccgctctcga tgcctacctc caggcctgcc tccacggcga ccccggggcc aacggctgcg
     2941 cccgcgccgc ggccatcgtc gccgcccgcg gcgacctgga gacagccatc cgctactatc
     3001 gtctctctaa ctggagtgaa gcgcgggcca tggccgacga actggagcgc cagctcgacc
     3061 cgtagcaacc ccagaggcag acacgccgcc cagcagacag acaggtaggt ctgccgctac
     3121 tccaaaacgg cgctataatc gcgcccatgt ccattctcaa tgccagccgc gtcggccagt
     3181 cattcggcga gttcgacgtc ttcagcggcg tcagcgtcag catcccgcgt gacggcaaga
     3241 tcggtctcgt cggccccaat ggcgtgggta agacgacact gctgctcatc ctggccggcc
     3301 gggcgcagcc gtcgtctggc gcggtccacg ttgcccgcgg cacgcgcatc ggctacctgt
     3361 cgcaggagtc gtccgatgct ttcgtcggcg cgatggccga gcagaccgtc tacgacgaga
     3421 tgctaaccgt cttcgccgag ctgcgggccg ttgaggagcg cctgcgcgtc ctggagcacg
     3481 agatgggcgc ggccgacgcc cacgacgcgt tgctggacga gtacagccag ttgcaagcgc
     3541 agtacgaagt tggcggcggt tatgagtacg acacgcgtgt gcgcatggtg ctgaccgggc
     3601 tgg
//
LOCUS       DAOSMQ010000327         3594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_25985_length_3594_cov_3.797400, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000327 DAOSMQ010000000
VERSION     DAOSMQ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25985_length_3594_cov_3.797400"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PK829_10675"
     CDS             <1..795
                     /locus_tag="PK829_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41718.1"
                     /translation="GQLNTLDLRAAGGRRVVVYNLGYPVMSLTKDLLLLDSALAYDAD
                     LVLWPVTLQSFARGRQLDHPLLQQNAARVRALIDHYDLALDPADPRLVTRGALAETLV
                     ARRRDLADLLRLQALGLAWAATGIDQDIPATIPPRRRDLDSDESWLDVAAPRPLTEAD
                     LALDVLRAGMARAEEAGVPLLLLNEPIYVSDGANSDIRYNTFYPRWAYDDYRAQLAAA
                     AGDAGWRYRDLWNAIPPAEFTDTPVHLTPTGTRLLAEILAPLVAGP"
     gene            complement(806..3271)
                     /locus_tag="PK829_10680"
     CDS             complement(806..3271)
                     /locus_tag="PK829_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU41719.1"
                     /translation="MATRAEKVDSPEKGANRRSLPDWLRTPGGMALVVSAFLACLAMW
                     AIWSELGQPFGGYVSYSFTENRPSIVASETPAWWPVLATRQLTLTDTLVAIDGQPYMA
                     NARRVYAAAAAEGRMVTVLAVNQETGVERLVTIEPTPITAADIVDVKLPESLIAIAFW
                     LLALLVLRARPASITNQVFAIAASAVAIHRAVALPSLFFDGQLLQLMATSGHLITAGL
                     IGPLLVHLALVFPTPLPRQRPRLLRTLYALGLASGVVGVATRLPWVVRVPLPVTLAVD
                     DGSYLGMLLLLLAGVVALFSRLVWSWFNERRTRRQRRATLIILLGLLTALPRVVIMLL
                     PLLRGLDDTIGTYWQGLDLRYLLLAIPIAFAFVIIRYQTFQALSALFILVLVLSISGA
                     LAAIGAWGWQILQPPGLLPTRPPFALLFVFIFLGSALWTIPLSRGRGLGRMFHWEAIT
                     YDAARMFGRRAMDATDARELPAVLTQALVEEMALERAALWLRGDGGRYALVGSAGDAG
                     MLPRDLAGPTAHETFPERALPGQPPEQLPAWLCSLGGEGRAEVVIPLSVEGQPLGLLG
                     LGRRWDEAIFDRRDLAMADLVGQQATLLLRAAMQMEELRRVPGQVAEAQERERLRVAG
                     ELHDTIQQFLGRLPFFLAVSRNSLSDDPQAAADILDRCLTDVEDAAAMLRRIRVNLAP
                     NLLETNLLRSLNGLVAHVGQRSGMAVTLSAPDDLDSALKPETRHALYRVIQQALDNTT
                     AHAEATTADVLLARLDGRVVFSVRDNGHGSTAEQRRSAEAAGSYGLRSMRARLEMCGG
                     YFALESEPGQGTTVRGWVPATDE"
BASE COUNT          653 a         1275 c         1137 g          529 t
ORIGIN      
        1 ggccagctca acaccctcga cttgcgcgcc gctggcgggc ggcgcgtcgt cgtctacaac
       61 ctgggctacc cagttatgtc cctgaccaaa gacctgctgc tgctggatag cgccctggcc
      121 tatgatgccg atctcgtcct ctggcccgtg acgctgcaat ccttcgctcg cggccggcaa
      181 ctggaccacc ccctgctgca acaaaacgct gcccgtgtcc gcgccctgat cgaccactac
      241 gacctggccc tcgacccggc cgacccgcgc ctggtgacgc gcggagcgct ggctgagacg
      301 cttgtcgccc gccgccgcga cctggccgac ctgttgcggc tgcaagcgtt ggggctggcc
      361 tgggcggcca cgggcatcga ccaggacatc cccgctacca tcccaccgcg ccgccgcgac
      421 ctggacagcg acgagagttg gctcgacgtg gctgcgcccc ggccgctgac agaggccgat
      481 ctcgccctcg acgtgctccg cgccggcatg gcccgcgccg aagaggctgg cgtccccctg
      541 ttgctgctca acgagcctat ttacgtcagc gatggagcca acagcgacat ccgttacaac
      601 accttctatc cgcgctgggc ctacgacgac taccgggcgc agttggccgc ggccgccggg
      661 gacgcgggct ggcgctatcg cgacttgtgg aacgccatcc cgccagccga attcacggat
      721 acacccgtcc acctgacccc aacgggcacg cggctgctgg cggagatact ggctccgctg
      781 gtcgccgggc cgtagagacg gccgctcact cgtcggtcgc cggaacccac ccccgcaccg
      841 tcgtgccctg ccccggttcg gattcgaggg cgaagtaccc gccgcacatc tccagccgcg
      901 cccgcataga cctcagccca tagctgccgg cggcctcggc gctgcggcgc tgttcggcag
      961 tggagccgtg gccgttgtcg cgcacgctga agaccacgcg gccgtccaac cgcgccagca
     1021 gcacgtcggc cgtggtggcc tcggcgtggg ccgtggtgtt atcgagcgcc tgctgaatga
     1081 cgcggtagag ggcatggcgc gtctccggct tcagggcact gtcgaggtca tccggcgcgc
     1141 tcagcgtcac ggccatgccg gaacgctggc ccacgtgcgc gacgagaccg ttaagcgacc
     1201 gcaacagatt cgtctctagc aaattggggg ccagattgac gcggatgcgg cgcaacatgg
     1261 ccgcggcgtc ctccacgtcg gtcaggcaac ggtcgagaat gtcggcggcg gcctggggat
     1321 catcgctcag gctattgcgg ctgacagcca ggaagaacgg cagacgcccc aggaattgct
     1381 ggatggtgtc gtgcagctcg ccggcgacgc gcaggcgctc gcgctcctgc gcctcagcca
     1441 cctggccagg tacgcgccgc aactcctcca tctgcatagc tgcccgcagc aacagcgtcg
     1501 cctgctggcc taccaggtcg gccatggcca ggtcgcgccg gtcgaagatg gcctcatccc
     1561 agcgtcgccc cagccccagc aggcccaaag gctgcccctc gacgctcagc gggatgacca
     1621 cctcggcccg cccctcgccg cccagtgagc acagccaggc cggtagttgt tcgggcggtt
     1681 ggccgggcaa cgcccgttcg ggaaaggtct cgtgggctgt cgggccggcc agatcgcgtg
     1741 gcagcatccc ggcatcgccc gcgctgccca ccaacgcgta acgaccgccg tctccacgca
     1801 gccagagcgc ggccctttcc agggccattt cctcaaccaa cgcctgggtc aggacggctg
     1861 gcaactcgcg cgcgtccgtc gcgtccatcg cccggcggcc gaacattcgc gccgcgtcgt
     1921 aggtgatggc ttcccaatga aacatccggc ccagcccacg cccgcgggac aaaggaatcg
     1981 tccacagagc actgcccagg aagatgaaca cgaacagcag cgcgaacggt ggacgtgtcg
     2041 gcaacaaccc cggcggttgg agaatctgcc acccccacgc gccaatggcc gccagcgcgc
     2101 cgctgatcga caggacgagc accagaatga acagcgccga aagcgcctgg aacgtttgat
     2161 agcggatgat cacgaaggca aaggcgatcg ggatggccaa caagagatag cgcaagtcca
     2221 gtccctgcca gtaggtgccg atggtgtcat cgagaccacg caacaagggc agcaacatga
     2281 tcaccacgcg cggcaaagcc gtcagcagcc cgagcaggat gatcagcgtg gcccgtcgct
     2341 ggcggcgcgt gcgccgctcg ttgaaccacg accagacgag gcgactgaac agggccacaa
     2401 cgccggccag caacaacagc agcatgccta aatacgagcc gtcgtccacg gccagcgtca
     2461 ccggcaaggg cacgcgcacc acccacggca ggcgcgtggc cacccccaca accccactgg
     2521 ccagaccgag ggcgtagagt gtgcgcagca gccgcggccg ctggcgcggc aacggcgtgg
     2581 ggaaaaccag ggccagatgg acaagaaggg ggccaatcag tccggcggtg atcaggtgac
     2641 ccgacgtggc catcaattgc agcaactggc cgtcgaagaa cagcgagggc aaagccacgg
     2701 cccggtggat ggccacggcc gacgccgcga tagcgaagac ctgattggtg atggacgccg
     2761 gccgggcgcg cagcaccagt agggccaaca accagaaagc gatggcgatg agcgattccg
     2821 gcaacttcac gtcaacaata tcggcggcgg tgatgggcgt tggctcgatg gtcacgaggc
     2881 gttccacgcc cgtctcctgg ttcacggcca gcacggtgac catgcgcccc tcggccgcgg
     2941 cggcagcata gacgcgccgc gcgttggcca tgtagggctg gccgtcaatg gccaccagcg
     3001 tgtcggtcag cgtcaattgt cgcgtggcca gcaccggcca ccaggccggc gtctctgagg
     3061 cgacaatgct agggcggttc tccgtaaagc tataggaaac atagccaccg aagggttggc
     3121 ccaactcgct ccagatcgcc cacattgcca ggcaggccag aaacgccgaa acgaccagcg
     3181 ccatgccgcc cggcgtgcgc aaccagtcgg gcaacgatcg ccggttggcg cccttttcgg
     3241 gactgtctac cttttccgcg cgcgtggcca tgtttcactc accggccgca ctcggcctac
     3301 caaacgctcc cgatacactc ctacgactct atcttagcgc cattttgcgc tcgccgcacg
     3361 gcctggcggc tttgcgtcac ggcttttcag ttgtcaccaa ttgacttgag acctgaccgg
     3421 ctgcatgcag tagcacagcc tgttaggctg tcgtcgccgg ccacagcctg accggctgca
     3481 tgcagtagca cagcctgtta ggctgttgtc accggccaca gcctgacagg ctgcatgcag
     3541 tagcacagcc tgttaggctg tcgtcgccgg ccacagcctg acaggctgtg ctac
//
LOCUS       DAOSMQ010000328         3589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26035_length_3589_cov_4.332201, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000328 DAOSMQ010000000
VERSION     DAOSMQ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26035_length_3589_cov_4.332201"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..641
                     /gene="thpR"
                     /locus_tag="PK829_10685"
     CDS             84..641
                     /gene="thpR"
                     /locus_tag="PK829_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOU41720.1"
                     /translation="MITIRAFIAIDLPAEVKQALGDVAAALAAGVPRGAVRWVRPEQI
                     HLTLRFLGDTPIDRLPALAAALDDVAGRQASFALRLTTTGCFPNAHRPRVVWVGLGGE
                     AAALAALVADLNAALGALGLPPEDKPFRAHLTLGRVKDGRGAQDMPLAVDVPALPIPV
                     AALHLIQSDLRPDGPVYTVRHVGRF"
     gene            complement(834..1064)
                     /locus_tag="PK829_10690"
     CDS             complement(834..1064)
                     /locus_tag="PK829_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41721.1"
                     /translation="MKQWEYAIAVEEAANWWTLWFQDAEDTPDTSARPDNHHSFRSDT
                     VMLGYMSREGWELATDIRLHDGLPRLLFKRRL"
     gene            complement(1109..2734)
                     /gene="tal"
                     /locus_tag="PK829_10695"
     CDS             complement(1109..2734)
                     /gene="tal"
                     /locus_tag="PK829_10695"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOU41722.1"
                     /translation="MRRVYVIMGVSGSGKTTVGQALARRLGVPFYDGDDFHPPENVAK
                     MAAGIPLTDEDRYPWLQTLHDLIASHLARGQAAVIACSALKEKYRDQLRAGNDGVYII
                     YLKGRPSLIARRLEDRQGHYMKAALLTSQLEALEPPSPHNSLIIDIGYNVDEIVRFVL
                     ANIAAEDAERQQGIVTMTNPLQKLYEYGQSFWYDNIRRAYLEDGTLAGLIANDGLRGL
                     TSNPTIFEKAITGDNDYDAAIGALVGRPATEVYDALSIADLRAACDLFLPLYRQSDRG
                     DGYASLEVSPYLAQDTKSTQSEARRLHAAVDRPNLMIKVPATDEGIPAIRALTAQGIN
                     VNVTLMFNMRHYEAVSAAYIAGLNDFVAAGGDPSGVASIASYFVSRVDTAVDALLERN
                     GGPRSLMGKAAVANSKVVYQRYKEIFHGEPFAALRAKGARPQRLLWASTSTKNPAYPD
                     TLYVDELIGPETVSTMPPQTITAFRDHGTLAYTLEADVDKARELLAELAAQGIDLDAV
                     TEKLQVDGVVAFSKSFDALLAAIVQKSDELAHV"
     gene            complement(3006..3539)
                     /locus_tag="PK829_10700"
     CDS             complement(3006..3539)
                     /locus_tag="PK829_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU41723.1"
                     /translation="MILETDRLTLRRLVPDDLDALFALYRDPEMRRYFPVEGASPDRT
                     LSREETKEELDWFLNGHPKHPELGLWATVLKENGAFIGRCGLLPWTLDGQDEVEVAYM
                     IAKAYWGRGLASEAARAIRDYAFTQLGLTRLVSFIDPANAASIHVAAAMGMTLEREAA
                     DEHGPFLVYSLTRPATP"
BASE COUNT          579 a         1159 c         1194 g          657 t
ORIGIN      
        1 ggagcagtac gttgtagggc gatttggcaa atcgccctac aataagagcg atttggcaaa
       61 tcgctctaca atagacgaga cgcatgatca ccatccgcgc ctttatcgcc atcgacctgc
      121 ccgccgaggt caagcaagcc ctgggcgacg tggccgcggc gctggcggcg ggtgtgccgc
      181 gcggggcggt gcgctgggtg cggccggagc agatacacct gacgctgcgc ttcttgggcg
      241 acacgcccat cgaccgcctg ccggccctcg ccgccgcgct cgacgacgtg gccgggcgac
      301 aggcgtcctt cgccctgcgg ctgacgacca ccggctgctt ccccaacgcc caccggccgc
      361 gcgtcgtctg ggtgggtctg ggcggggagg cggcggcgct ggcggcgctg gtggccgacc
      421 tcaacgccgc cctcggcgcg ctaggcctgc cgccggagga caaacccttc cgcgcccacc
      481 tgaccctcgg ccgcgttaag gacggccgcg gcgcgcaaga catgcccctg gccgtcgatg
      541 tgcccgcgct gcccatcccc gtcgccgccc tgcacctcat ccagagcgac ctgcggccgg
      601 atgggccggt gtatacggtg cggcatgttg gcaggttcta aggagtaggg acaggggacg
      661 agggaacgcg gaagccggag ccactgtcgc ccctagtccc cgtcaaacgg cgagcgcggc
      721 gtggccggcc tcagccgcgg cggctggctc tacgccccag caactgcctg atcgctcgct
      781 attcgtgctc ctggcccggc ggcaaggcga accacatcgg cgcggtcgtc ccgctagagg
      841 cggcgcttaa agagcaggcg cggcagccca tcgtgcaggc ggatgtcggt cgccagttcc
      901 caaccctcgc gcgacatata gccgagcatg actgtgtcgc tgcggaagct atgatggtta
      961 tcggggcgcg cgctcgtgtc cggcgtgtcc tccgcgtcct gaaaccacag cgtccaccag
     1021 ttagccgctt cttcgacggc gatggcgtat tcccattgtt tcatgcccaa cccctcctgg
     1081 ccgaacgacc gtccgcgcgt aggcccgcct atacgtgagc cagttcgtcg ctcttctgga
     1141 cgatggccgc cagcagggcg tcaaacgact tgctgaaggc gacgacgcca tcaacctgaa
     1201 gcttctcggt gacggcatcc agatcgatgc cctgcgcggc caactcagcc agtaactctc
     1261 tggctttgtc tacgtccgct tccagcgtgt aggccagggt gccgtggtcg cggaaggcgg
     1321 tgatcgtctg cggtggcatg gtcgagacgg tctccggacc gatgagttca tcgacgtaga
     1381 gggtgtcggg ataggcggga ttcttcgtgc tcgtcgaggc ccagagcaag cgctgcggcc
     1441 gcgccccctt ggcccgcaag gcggcgaagg gttcgccgtg gaagatctcc ttgtagcgct
     1501 ggtagacgac tttggagttg gccacagcgg ccttgcccat caagctccgg gggccgccgt
     1561 tgcgttccag cagcgcgtcc acggccgtgt ccacccggct gacgaagtag gaggctatcg
     1621 aggccacgcc cgaagggtcg cccccggcgg cgacaaagtc attcagtccg gcgatgtagg
     1681 ccgcggacac ggcctcgtag tggcgcatgt tgaacatcag cgtcacgttg acgttgatgc
     1741 cctgggccgt aagggcgcga atggcgggaa tgccctcgtc ggtggccggc accttgatca
     1801 tcaggttcgg ccgatcgacc gccgcgtgca gccgtcgggc ctcggactgc gtgctcttgg
     1861 tgtcctgcgc cagatagggc gatacctcca ggctggcgta gccgtcgccg cggtcggact
     1921 ggcgatagag cggcaggaac aggtcgcagg cggcgcggag gtcggctatc gacagcgcgt
     1981 cgtagacttc ggtcgccggc cgccccacca acgcgccgat ggcggcgtcg tagtcgttgt
     2041 cgccggtgat cgccttctcg aagatggtcg ggttcgatgt caggccacgc aggccgtcat
     2101 tggcaatcag gccggccagc gtgccgtctt ccaggtaggc gcggcggatg ttgtcgtacc
     2161 agaagctctg gccatactca tacagtttct gaagcggatt ggtcattgtg acaattcctt
     2221 gttgcctttc ggcgtcttcg gcagcaatgt tcgccaggac gaaacggaca atctcatcga
     2281 cattgtaacc gatgtcgatg atgaggctgt tgtggggcga gggcggttcc agcgcctcta
     2341 actggcttgt cagcaaagcg gccttcatgt agtggccctg tcggtcttcc aggcgtcggg
     2401 cgatgaggct cggccgcccc ttcagataga tgatgtagac gccatcgttc cccgcgcgca
     2461 gttggtcgcg gtacttttcc ttcagggctg aacaggcgat caccgccgcc tggccgcgcg
     2521 ccagatggct ggcgatgagg tcgtgcaggg tctgtagcca gggatagcgg tcctcgtccg
     2581 tcagcgggat accggcggcc atcttggcca cgttctccgg cgggtggaag tcgtcgccgt
     2641 cgtagaaggg cacgcccagc cgccgcgcca gcgcctggcc gacagtggtc ttgccactgc
     2701 cggacacgcc catgatcacg tacacacgcc tcatcttgcc tcttgggcct gacgcctaag
     2761 ccccggcccg tccggggcag ttagagaata cccgtgtcgg cgcgacgcgg ccactgcggt
     2821 cactgccaaa agtcaataga agctcacgcc aaagcgcaaa gggggccaaa ggcgcaaaga
     2881 aatgagcaaa ggcttgagat tcttctttgc gtcttttgct tacttcgcgc cctggcgtga
     2941 gcgctccttc cactttgcag tgaccccgtg atgactgcca tttcggttgc cacaaatctc
     3001 aatgctcatg gagtcgccgg ccgcgtcagg gaatagacca ggaacggccc gtgctcgtcg
     3061 gccgcctctc gctccagggt catgcccatg gccgcagcca cgtggatcga cgccgcgttg
     3121 gccgggtcaa tgaagctgac cagacgggtc aggcccagct gcgtgaaggc gtagtcgcgg
     3181 atggcgcggg ctgcctcgct ggccaggccg cggccccagt aagccttggc gatcatgtag
     3241 gccacttcga cctcgtcctg gccgtcaagc gtccacggca gcaggccgca gcggccgatg
     3301 aacgcgccgt tctccttcag caccgtcgcc cacaggccca actccgggtg cttggggtgg
     3361 ccattgagga accagtccag ttcctctttc gtctcctcgc ggctcagcgt ccggtcgggc
     3421 gacgcgccct ccactggaaa gtagcggcgc atctccgggt cgcggtagag ggcgaagaga
     3481 gcatcgaggt cgtcggggac gaggcggcgg agggttaggc ggtcggtttc caggatcatc
     3541 attggcctgt ttccttgagg tatgctactg gatgatgtat tatacgatg
//
LOCUS       DAOSMQ010000329         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26064_length_3587_cov_4.486127, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000329 DAOSMQ010000000
VERSION     DAOSMQ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26064_length_3587_cov_4.486127"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PK829_10705"
     CDS             complement(<1..978)
                     /locus_tag="PK829_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU41724.1"
                     /translation="MTEMPKAYDFSGTEERLYQWWEENGWFKPEARPADAEPFVISIP
                     PPNVTGELHMGHAMFVALEDLMIRRARMQGRAALWVPGTDHAGIATQLQVERMLRDEG
                     TSRAEVGRDEFLRRTWEWKARYGGQITHQLRRLGASCDWERERFTLDEGLSAAVREAF
                     VRLYRLGLIYQDEYLVNWSPGLQTAVSDLEVEYSEEQGLLYYFQYPVAGGGFLPVATT
                     RPETILGDTAVAVHPDDARYAALVGQMALVPILNREIPIIADPYVDMDFGTGALKVTP
                     GHDPKDFEIGQRFGLPVISVMNKDATMSAAAGPYAGLDRFECRERLWADM"
     misc_binding    complement(1064..1292)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1460..2692)
                     /locus_tag="PK829_10710"
     CDS             complement(1460..2692)
                     /locus_tag="PK829_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41725.1"
                     /translation="MSERRANRLAALGLLWAFGVTLGRGLRRPNDWAEAHWLISYDFG
                     FIKRGLAGSLLAPFLSPMPAVAEATITVVATLLTALLCAALVALCWSILRRCGFSAEA
                     VLAVAVFVTSPFVVMTGHLNGYFDAPIILLSVLAVSLAWRGRLWPAALVLTVGLLVHE
                     TILIIGFPTALWAAWLRFGRDEATTAARLRRVAPLAMPLLAFALLFLYQSYVIDAARL
                     ESTLIAYLQKQPFIQYDQEIIVPRSFAKSFTAQFQSQSPRAWGRLFDRGLIVAVVPSL
                     LVWLVFIGNALRAGAVSRRLMVVGGLLPFLPLALHLIAWDTGRIWTYPLVVALLVGWV
                     ACRAAAPARLRAARSAVLPVLALVALPINVFGRIALMDWRVERFSAAARAALYAPLAV
                     ALAGALQSRKKRSRQAAK"
     gene            complement(2694..>3587)
                     /locus_tag="PK829_10715"
     CDS             complement(2694..>3587)
                     /locus_tag="PK829_10715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU41726.1"
                     /translation="GRGSDVETIVHMGAVSSTTEPDADKIIHSNFTLSRDLFDWSAEH
                     NRRMIYASSAATYGDGSLGFEDDDSLEALEQLRPLNAYGWSKALFDVYAARKAARGQA
                     PPQWTGLKFFNVYGPNEGHKGGMKSVVAQVWPTVRDGGQVQLFQSHNPSYADGGQMRL
                     RLFDDEAPTTVNNFVYLANQGFYDGTTFHRVLENFMAQGGDPSGTGSGGPGYTFQDET
                     DNGLTFDRRGLLAMANSGPDTNGSQFFITFAPATHLDGLHTIFGELTEGDDVLSGITL
                     RDPDTATAPGDVIEEITIFEQ"
BASE COUNT          625 a         1180 c         1229 g          553 t
ORIGIN      
        1 catgtcggcc cacaggcgct cgcggcactc gaagcgatcc agcccggcgt atggcccggc
       61 ggcggcgctc atggtggcgt ccttgttcat caccgagatg acgggcaggc cgaagcgctg
      121 accgatctcg aagtccttgg ggtcgtggcc gggcgtcacc ttcagcgcgc ccgtgccgaa
      181 atccatatcg acgtaggggt cggcgataat ggggatttcg cggttcagga tgggcacgag
      241 ggccatctgg ccgacgagag cggcgtagcg cgcgtcgtcg gggtggacgg ctacggccgt
      301 atcgcccaga atcgtctccg gccgcgtggt ggccacgggc aggaagccgc cgccggccac
      361 ggggtactgg aagtagtaga gtaggccctg ctcctcgcta tactccactt ccaggtcgga
      421 cacagccgtc tgtaggccag gcgaccagtt gaccagatac tcgtcctggt agatgaggcc
      481 caggcgatag aggcgcacga aggcctcgcg cacggccgcc gacaggccct cgtcgagcgt
      541 gaagcgctcg cgctcccagt cacacgacgc gcccaggcgg cgcagctggt gggtgatctg
      601 gccgccatac ctggccttcc attcccaggt gcggcgcaga aattcgtcgc ggccgacctc
      661 cgcccgcgag gtgccctcgt cgcgcagcat ccgctccacc tgtagctggg tggcaatgcc
      721 ggcgtggtcg gtgcccggaa cccatagcgc cgctcgcccc tgcatccgcg cgcggcggat
      781 catcaggtct tccagagcga cgaacatggc gtggcccatg tgcaactcgc cggtgacgtt
      841 gggcggcggg atggagatga caaatggctc ggcgtcggcc gggcgcgctt cgggcttaaa
      901 ccagccgttc tcttcccacc actggtagag cctctcttcc gtcccgctga agtcataggc
      961 ctttggcatt tcagtcatgc gtctatcctc ttatatgcgc ccgcgggccg cgcggcccgt
     1021 gggggcgatg atgaccaaat gaaatgaaaa acgctctcgc ctgttcaagg gcgagagcgt
     1081 actgctcgcg tggtaccacc ttgcttccgg cgcgggtgac gcgccggcac tccaacggcg
     1141 ataacgggcc ggcccgcccg cgcctacttg acgttcagca cggggactcc tgggcgactt
     1201 cggcagcggt gctccggcgc tggctctcaa ccatgaccag cgctctctgt cgggcaaata
     1261 ggccgcctac tcctcccaat cacggtcttt cggcagtgaa ttgtcgatcg ctgctagtat
     1321 agtcgccgcc gcggcagcgc gcaagggtga gagccagggc caccagggtc attgcaaagt
     1381 cgatagaagc tcacgcgaag gggcaaaagg cgcaaagaag aaatggccgg cgatctgcct
     1441 gcttcctttg cgtctttgct tacttggcgg cttggcgtga gcgcttcttc cggctttgca
     1501 gtgccccggc cagggccacg gccagcggag catagagcgc ggcccgcgcc gcggccgaga
     1561 agcgctccac ccgccagtcc atcagcgcga tacgcccgaa gacgttgatc ggcagggcga
     1621 ccagggccag gacgggcagc acggccgaac gcgccgcccg caaccgcgcc ggtgcggccg
     1681 cccggcaggc cacccagccc accagcagag cgacgacgag cgggtacgtc cagatgcggc
     1741 cggtgtccca ggcgatcaag tgcagcgcca gcggcaggaa gggcagcagc ccgcccacga
     1801 ccatcagacg acgcgagacc gcccccgccc gcagggcgtt gccgatgaac accagccaca
     1861 ccagcagcga cggcacgacg gcgacaatca gtccccggtc gaacagccgt ccccaggcgc
     1921 gtgggctttg cgactggaat tgggccgtga aggacttggc aaacgagcgc ggcacaatga
     1981 tttcctggtc gtactggata aagggctgct tttgcagata ggcgatgagc gtgctctcca
     2041 gccgggcggc gtcgatgacg taggactggt agagaaacag cagcgcgaag gccagcaggg
     2101 gcatggccag cggcgcgacc cggcgcagac gcgcggccgt tgtggcctcg tcgcgcccga
     2161 agcgcagcca ggccgcccag agggccgtcg ggaagccgat gatcaggatg gtctcatgga
     2221 cgagcagccc tacggtaagg acgagcgccg ccggccacag ccgcccgcgc caggccaggc
     2281 tgacggccag cacgctcagc aggatgatcg gcgcgtcgaa gtagccgttc aggtggccgg
     2341 tcatgacgac gaagggcgag gtgacgaaga cggccacggc cagcacagcc tcggccgaga
     2401 agccgcaacg gcgcaggatc gaccagcaca gagccacgag cgcggcgcac agcagcgccg
     2461 tcagcagagt ggccaccacc gtgatggtcg cctcggccac ggccggcatt gggctgagga
     2521 atggggccag caggctgccc gccaggcccc gtttgatgaa gccgaagtcg tagctgatca
     2581 gccagtgggc ttcggcccaa tcgttcggcc gccgcagccc gcgccccagc gtcacgccga
     2641 aggcccacag tagccccaac gccgccagcc ggttcgcgcg ccgctcgctc atgctactgc
     2701 tcgaagatgg tgatctcctc gatgacgtcg cccggcgcgg tggctgtgtc cgggtcgcgc
     2761 agggtgatgc cgctgaggac atcatccccc tcggtcagct cgccgaagat ggtgtgcagc
     2821 ccgtccagat gggtcgccgg ggcgaaagta atgaagaact ggctgccgtt ggtatctggc
     2881 ccggagttgg ccatggccag caggccgcgg cgatcgaagg tcaggccgtt gtcggtttcg
     2941 tcctggaagg tgtagccggg gccgccgcta cccgtgccgc tggggtcgcc gccctgggcc
     3001 atgaaattct ccagcacgcg gtggaaggtc gtgccgtcgt agaaaccctg gttggccagg
     3061 tagacgaaat tgttgaccgt ggtgggggcc tcatcgtcaa agagccgcag gcgcatctgg
     3121 ccgccgtcgg cgtaagacgg gttgtgcgat tggaagagct gcacctggcc gccgtctcgc
     3181 acggtcggcc agacctgcgc cacgaccgac ttcatgccgc ccttgtggcc ctcgttgggg
     3241 ccgtagacgt tgaagaactt caggcccgtc cactgcggcg gcgcctgacc gcgcgccgcc
     3301 ttgcgggcgg cgtagacatc gaacagcgcc ttggaccagc cgtaggcgtt gaggggccga
     3361 agctgctcaa gcgcttcgag gctgtcgtcg tcctcgaagc cgaggctgcc atcaccatag
     3421 gtcgccgccg aggaggcgta gatcatccgc cgattgtgct cagcgctcca atcgaacaga
     3481 tcgcgcgaca gggtgaagtt ggaatggatg atcttgtcgg cgtcgggctc cgtggtcgat
     3541 gagaccgcgc ccatgtggac gatggtctcg acgtccgacc cgcggcc
//
LOCUS       DAOSMQ010000330         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26068_length_3587_cov_3.979615, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000330 DAOSMQ010000000
VERSION     DAOSMQ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26068_length_3587_cov_3.979615"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2034)
                     /locus_tag="PK829_10720"
     CDS             complement(<1..2034)
                     /locus_tag="PK829_10720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU41727.1"
                     /translation="MHLPAARRRLHTRLLAVAALLVFFTLALDTAGRKAITTDEPLHL
                     VHTLTLAQTGAMRIPEMHTPLTYRLVGDLLRTEPALPAVAGMATWPTQNPYDIGREYI
                     RRDDVAIDQVMWLSRFVVVLMGVVLGALMLSWMCALSRSPVAAVTVAGLYALSPNLLA
                     SAALLTTDMAATVTWFATIYAWWRYWQRPGPGRWLAAAVALGLALAAKLTGVLLLPVT
                     LLLAYAGAPRGAPWRRTLLVWLGLLPPAALVLWALYGFQTRAGLPLPAYWTAWDMLLS
                     EVAVGHSNFFLGRISMIGSWFYFPVTLLLKTPPLQLALFLLIPVVLWRERARWRVWAF
                     PVLAAVVLLVVAAASRFNFGYRHLLPAVPFLMLLGGAAAAWLWELASPRPRLVARLAL
                     SLAAAGTVVATARTHPNYLAYFNALARGQGERYLGDSNLDWGQDLRLLADYARQYEAA
                     TGQRLRFSYSGIAGREREGLAGPSLVEAFNAGEPFPAANPPAGRYAINASDLQGTGLV
                     LGTLTEIDLFDWFRRREPMATLGGSIFLYDVAAPAGGQWVAHCAAPGRLLDDAAAERL
                     VGRRGLRHVTFDCRTSWVFPGETPGWYVLPPGVTWLDDWAAWQRPEVVYRHRANAYGP
                     AYEIAYWPGGGGGPLLSPCAGEIDSCAGQLLRRHDGQSGVATLRGYGARAVEWL"
     gene            2284..>3587
                     /locus_tag="PK829_10725"
     CDS             2284..>3587
                     /locus_tag="PK829_10725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU41728.1"
                     /translation="MKKRFVVSAWAVLLTIVLLLAACGPAQPPADATAEAGGETATTT
                     TDEATVDETTDSGDLEATPAGIPTVEGPTTTTDSGLIYIETQAGDGRTPEEGDIVTMN
                     IVGMLDDGTVFADTYTDGTPITATATDEDLFPGWKEGLLLMKEGGKARLIVPPELAFG
                     SEGAGGVIPADATVTMDVELISAESPPVPTAVEESDLTTTDTGLQYFDIVKGEGDMPQ
                     VGQEVVINYVAWLQEGEVFIARSDDQGGELTFAIGSDTVFPGWEEGVTTMQKGGKRLL
                     IIPPDLALGDEGGGRIPPGATLLMEVELVDILPLVTPTAVEESDFTVTESGLKYYDLV
                     EGDGAVAEAGSDVTVNYTGWLTNNVKFDSSLDAGQPFTFTLGTSAVIPGWDEGLAGMK
                     VGGKRQLVIPAELGYGATGGGSIPPNATLIFEVELLDVQPAAT"
BASE COUNT          649 a         1205 c         1264 g          469 t
ORIGIN      
        1 gagccactcc accgcgcgcg cgccgtagcc ccgcagagtg gccaccccgc tctggccgtc
       61 gtggcggcgc aggagctggc ccgcgcagga gtcgatttcc ccggcgcacg gcgacaatag
      121 cggcccacca cccccgccgg gccagtaggc gatctcatac gccggcccgt aggcgttggc
      181 ccggtggcga tagaccactt ccggccgctg ccacgccgcc cagtcgtcga gccacgtcac
      241 ccccggcggc aggacgtacc agccgggcgt ttcgccgggg aagacccagc tggtgcgaca
      301 gtcgaaggtg acgtggcgca ggccgcggcg gccgaccagc cgctcggcgg cggcgtcgtc
      361 gagcagccgg ccgggcgcgg cgcagtgggc cacccactgc cccccggccg gcgcggccac
      421 gtcgtagagg aagatggagc cgcccagtgt ggccattggc tcgcggcggc ggaaccagtc
      481 gaacagatcg atctcggtca gcgtgcccaa caccaggccc gtgccctgca ggtcgctggc
      541 gttgatggcg tagcggccgg cgggcgggtt ggccgccggg aacggctcgc cagcattgaa
      601 cgcctccacc agcgacgggc cggccagtcc ctcgcgttcc cggccggcga tgccgctgta
      661 ggagaagcgc agccgttgac cggtggcggc ctcatactgg cgggcgtagt cggccagcag
      721 gcgcaggtct tgcccccagt cgagattgga gtcgcccagg tagcgctcgc cctggccgcg
      781 ggccagcgcg ttgaagtagg ccaggtagtt ggggtgggtg cgcgcggtgg cgacgaccgt
      841 gccggccgcg gccagcgaca gggccaggcg ggcgaccagg cgcggccggg gcgacgccag
      901 ttcccacagc caggccgccg ctgccccgcc caacagcatc aggaagggca cggccggcaa
      961 cagatggcga tagccaaagt tgaagcggct ggccgcggcc acgaccagca gcacaaccgc
     1021 cgccagcacg ggaaaggccc acacccgcca ccgcgcccgc tcgcgccaca ggacgacggg
     1081 gatgagcagg aagagcgcca gttgcagcgg cggcgtcttc agcagcagcg tcaccgggaa
     1141 atagaaccac gagccgatca tgctgatgcg gccgaggaaa aagttgctgt ggccgacggc
     1201 gacctcgctg aggagcatgt cccaggccgt ccagtaggcc ggcaggggca acccggcgcg
     1261 ggtctggaag ccatagagtg cccagagcac gagggccgcc ggcggcagca gccccagcca
     1321 caccagcagc gttcgccgcc acggcgcgcc ccgcggcgcg ccggcatagg ccaacagcag
     1381 tgtgaccggc agcagcagca cgccggtcag cttggccgcc agcgccagcc ccagggccac
     1441 ggccgccgcc agccagcggc cggggcctgg ccgctgccag tagcgccacc aggcgtagat
     1501 cgtggcgaac cacgtcaccg tcgcggccat gtcggtggtt agcagcgcgg ccgaggccag
     1561 caggttgggc gagagggcat agaggccggc cacggtgacg gcggccacgg ggctgcggct
     1621 gagcgcacac atccacgaaa gcatcagcgc gcccaggaca acgcccatca gcacgacgac
     1681 aaagcggctc agccacatga cttggtcgat ggccacgtcg tcgcgccgga tatactcgcg
     1741 gccgatgtca taggggttct gcgtcggcca ggtggccatg ccggctacgg cgggcagcgc
     1801 cggctcggtg cgcaacaggt cgcccacgag ccggtaggtc aggggcgtgt gcatctccgg
     1861 gatgcgcatc gcgccggtct gcgccaacgt cagggtgtgg acgaggtgca gcggctcatc
     1921 ggtggtgatg gccttgcgcc cggccgtatc cagcgccagg gtgaagaaga ccagcagcgc
     1981 tgccacggcg agcaggcggg tgtggaggcg acgacgcgcg gccggcaggt gcatggagcg
     2041 gattataccc ctgactgggc gaataagggt cagtgaacgg tggttagtgc ccagcaagac
     2101 gccaccgcgc gccggccagc gatcactgac caccgggttt ctgtgtaaca ggtgaaataa
     2161 cggcttctac aggccaggga tgtggcacgc ggcgcgggga gaagatacca ttgggcagcc
     2221 atcctgaaaa tgccacggcc aaacgccgcc gcagtttcat cagaacagga gtttgagact
     2281 ggaatgaaga agagatttgt ggtttccgcg tgggccgtat tgctcacgat tgtgctgctg
     2341 ctggccgcgt gtggcccggc ccagccgccg gccgacgcca cggccgaagc tggtggcgag
     2401 acggcaacaa cgacaaccga tgaggccacc gtcgatgaga ccaccgacag cggtgatctt
     2461 gaagcgacgc cggccggcat ccccactgtc gaagggccga cgaccacgac cgacagcggc
     2521 ctgatctaca ttgagacgca ggccggcgac ggccgcacgc ccgaagaggg cgacatcgtc
     2581 actatgaaca tcgtcggcat gctcgatgac ggcacggtgt tcgccgacac ctacaccgat
     2641 ggtacgccca tcaccgccac cgccaccgac gaggacctct tccccggttg gaaagagggc
     2701 ctgctgttga tgaaagaagg cggcaaagcc cggcttatcg tcccgcccga actggccttt
     2761 ggctccgagg gcgcgggcgg cgtcatcccc gccgacgcca ccgtgacgat ggacgtcgaa
     2821 ctcatcagcg ccgaatctcc gccggtgccg acggctgttg aagagagtga cttgacgacg
     2881 accgacaccg gcctgcaata ctttgacatc gtcaaaggtg agggcgatat gccccaggtg
     2941 ggccaggaag tggtcatcaa ttacgtggcc tggttgcagg aaggcgaggt cttcatcgcc
     3001 cgctctgacg accagggcgg cgagctgacc ttcgccattg gcagcgacac cgtcttcccc
     3061 ggctgggaag agggcgtgac cacgatgcag aagggcggca agcgcctgct gatcatcccg
     3121 cccgatctgg ctctgggcga cgagggcggc ggccgcatcc cccctggggc gacgctgctg
     3181 atggaagttg aactggtcga catcctgccg ttggtgacgc caacagccgt cgaagagtcc
     3241 gacttcaccg tcaccgagag cggcctgaag tactacgacc ttgtcgaggg cgatggggcc
     3301 gtggccgagg cgggcagcga cgtgaccgtc aactacaccg gttggctgac caacaacgtc
     3361 aagttcgata gctcgctcga cgccggccag ccgttcacct tcaccctggg caccagcgcc
     3421 gtcattcccg gctgggacga gggcctggcc ggcatgaagg tcggcggcaa gcgccagcta
     3481 gtcatcccgg cggagttggg ctatggcgcg accggcggcg gcagcatccc gcccaacgcc
     3541 acgctcatct ttgaggttga gttgctggac gtgcagccgg cggcaac
//
LOCUS       DAOSMQ010000331         3575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26188_length_3575_cov_4.777841, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000331 DAOSMQ010000000
VERSION     DAOSMQ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26188_length_3575_cov_4.777841"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PK829_10730"
     CDS             complement(<1..432)
                     /locus_tag="PK829_10730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU41729.1"
                     /translation="MLDSIDESVAVPGAPALPGLAFRRFRGPSDYAALAAVGNASRQA
                     DDEEWGMSVEELAAEFDHPVNRDPRRDMVIAEIDGEMIGFGQGRWSVDASGLYRYFPE
                     AELLPEWRGHGLRRALLRWLEARLRQVAAEHPATAEKVFRAQ"
     gene            complement(607..1272)
                     /locus_tag="PK829_10735"
     CDS             complement(607..1272)
                     /locus_tag="PK829_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41730.1"
                     /translation="MDIYLEQGAKRTFAGALEWPGWCRAGRDEAAARQALLDYAPRYA
                     AVAARAGLDFAPPDAAAGFRVVERLPGDATTDFGAPSAIPASDRERLREGELARLGAL
                     LAGCWGVFDAVAAAASGVTLRKGPRGGGRELEDITRHVLGAEQGYLARLAWKAPRGDA
                     NLNEDMRRTRQAIMDALAAAERGEIPERGPRGGVIWPARYFVRRVAWHVLDHAWEIED
                     RAV"
     gene            complement(1338..1913)
                     /locus_tag="PK829_10740"
     CDS             complement(1338..1913)
                     /locus_tag="PK829_10740"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOU41731.1"
                     /translation="MELMTQRLRLREFREDDWLAVLAYQSDPLYLRYYEWTERTPEAV
                     RAFIGMFLKHQRTQPRTRFQLAAILRATGELIGNCGVRLTAPGSHEADLGYELSPAHW
                     GHGYATEAARAMLNFAFTQLGVHRVWAEAVVNNTGSTHVLAKLGMQLEGRLRDKEFFK
                     DRYWDTVIYAILEQEWRARQEATLMDDPRAH"
     gene            complement(1995..2570)
                     /locus_tag="PK829_10745"
     CDS             complement(1995..2570)
                     /locus_tag="PK829_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HOU41732.1"
                     /translation="MSRTRLEAFSDGVIAILITIMVLQLTPPHGHDLAALRPLVPVFL
                     GYVLSFLYLGIYWTNHHHLFQVVEQVDGRVLWANIHLLFWLSLVPFLTAWVGDNGLAA
                     LPVALYGLVLLASAIAYYFLTRALLALHRPNSALAKALQGDFKEKVSIALYVVGIVLA
                     FVNAWLGCAAYVLVALIWLIPDRRVEKVLGQ"
     gene            complement(2590..2886)
                     /locus_tag="PK829_10750"
     CDS             complement(2590..2886)
                     /locus_tag="PK829_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOU41733.1"
                     /translation="MIRVIIPHHLRNLAGVGAEVSVAVAEPVTQRAVLDALEARYPVL
                     RGTIRDYSTGQRRAYLRFFAGAEDLSHEAPDAPLPPDVANGKEPLRIIGAIAGG"
     gene            complement(2883..>3575)
                     /locus_tag="PK829_10755"
     CDS             complement(2883..>3575)
                     /locus_tag="PK829_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOU41734.1"
                     /translation="PRLRQHDTGGIWQPGAGGLCLHTILLDPSNPERIVVAISAAGAF
                     RSDDGGQTWKAINQGLRSDYIPDPKAEVGHCVHHVAMHPSNPDRLFMQKHWDVMRSDN
                     AGDEWYEISGSLPSDFGFVIDVHAHEPETIYVVPIKSDSEHYPPDGKLRVYRRRGGNE
                     WEALTDGLPQENCYVNILRDAMSVDKLDPCGIYFGTTGGQVYVSADGGDHWQAIVHDL
                     PAVLSVEAQLLP"
BASE COUNT          598 a         1191 c         1231 g          555 t
ORIGIN      
        1 ttgggcgcgg aagaccttct cggccgtggc cggatgctcg gcggcgacct gtcgcaggcg
       61 cgcttccagc cagcggagca gcgcccggcg aaggccgtgt cctcgccatt ccggcagcag
      121 ctctgcctcc gggaaatagc gatacaggcc ggaagcgtcc actgaccagc ggccctggcc
      181 gaagccgatc atctcgccgt cgatctcggc gatcaccatg tcgcgtcggg gatccctgtt
      241 aaccgggtgg tcgaattcgg ccgccaattc ctccaccgac ataccccatt cttcgtcatc
      301 ggcctgtcgt gaggcattgc cgacggcggc aagagcggcg taatcgctcg ggccgcgaaa
      361 acggcggaaa gccagcccgg gcagggcggg ggcgccgggc acggcgacgg attcatcaat
      421 cgagtcgagc atagcggttg acctccagcg aagggtacac agtcgggcgc gacgagacac
      481 gcggatcaca aggttagcat agcggcgctg ccggcgctgt catgcaggct acacacccgg
      541 cggcaggacg ggcaggctac agagatgggt cagttctggc cccgggtaag cgcctgtctc
      601 gcgccctcag acagcgcgat cctcgatctc ccaggcgtgg tcgagcacgt gccaggccac
      661 gcggcggaca aagtagcgcg ccggccagat gacgccgccg cgcgggccgc gctccgggat
      721 ttcgccgcgc tcggccgcgg ccagcgcgtc catgatcgcc tgccgcgtcc ggcgcatgtc
      781 ctcgttcagg ttggcgtcgc cgcgcggggc cttccaggcc aggcgggcga gatagccctg
      841 ctccgcgccc agcacgtggc gggtgatgtc ctccaactcg cgtccgccgc cgcgcggccc
      901 cttgcgcagg gtcacgccgc tggccgccgc ggcgacggcg tcaaagacgc cccagcagcc
      961 ggccagcaac gcccccagtc gagccagctc gccctcgcgc agccgctcgc ggtcgctggc
     1021 ggggatggcg ctgggcgcgc cgaagtcggt cgtggcgtcg cccggcaggc gctcgacgac
     1081 gcgaaagccc gcggccgcgt ccggcggggc gaaatccagc ccggcccgcg cggccacggc
     1141 ggcgtagcgc ggggcatagt cgagcagcgc ctgccgcgcg gcggcctcgt cgcgccccgc
     1201 ccggcaccag cccggccact ccagcgcccc ggcgaacgtg cgcttggcac cctgttccag
     1261 atagatgtcc atcgtcctta ttcctcagcc ttggcccacg cgaggtagcc gggtgcagtc
     1321 atctggcccg gcgcggccta atgggcgcgt gggtcatcca ttagggtcgc ctcttgccgt
     1381 gcgcgccact cctgctccag gatggcgtag ataaccgtat cccagtagcg atccttgaaa
     1441 aactccttgt cgcgtagccg tccctccaac tgcataccca gcttcgccag cacatgggtc
     1501 gagccggtgt tgttaacgac ggcttcggcc cagacgcgat ggacgcccag ctgggtgaaa
     1561 gcgaagttga gcatggcgcg ggccgcctca gtggcgtagc cgtgccccca gtgggccggt
     1621 gacagctcat agcccaggtc ggcctcgtgg gagccgggcg cggtcagacg cacgccgcag
     1681 ttgccaatca actcgccagt cgcccgaagg atggcggcta actggaatcg ggtgcgcggt
     1741 tgcgtgcgct ggtgcttgag aaacatgcca atgaaggcac gcaccgcttc gggcgtgcgt
     1801 tccgtccact catagtagcg caggtagagt gggtcggact ggtaggccag cacggccagc
     1861 cagtcgtctt cgcggaactc gcgcagccgc aagcgttggg tcatcagttc cataggcgtc
     1921 ctcggtcagc aggactcaaa cagtgctcag gtagtaggcc ttcgcatagg ccttggcaac
     1981 tcggggcgca gcccctattg gcccaacacc ttttccacgc gccggtcggg aatgagccag
     2041 attaaagcca cgagaacata ggccgcgcag cccagccagg cgttgacaaa ggccaggacg
     2101 atgcccacga cgtagagggc aattgacacc ttctccttga agtcgccttg cagcgctttg
     2161 gccagcgccg aattgggacg atggagcgcc aacagggcgc gcgtcaggaa gtaataggcg
     2221 atagccgagg ccaggagaac cagcccataa agcgccacgg gcagcgccgc cagtccattg
     2281 tcgcccaccc aggccgtcag gaacggcacc agcgacagcc agaacaacag gtggatgttg
     2341 gcccacagca cgcggccgtc cacctgctct acgacctgaa acaggtgatg gtggttagtc
     2401 cagtagatgc ccagatagag gaagcttagc acatagccca ggaacacggg cacgagcggc
     2461 cggagcgcgg ccagatcgtg gccgtgcggc ggcgtcaatt gcagcaccat gatggtgata
     2521 aggatggcaa tgacgccgtc gctgaaggcc tccagtcgtg tcctgctcat gggctacttg
     2581 cgttcgccat tacccgccgg caatcgcgcc gatgatgcgc aacggctctt tgccgttggc
     2641 gacatcaggc ggcagcggcg cgtccggcgc ttcgtgcgac aggtcttccg cgccggcgaa
     2701 gaagcgcagg taggcgcggc gctgccccgt gctgtagtcg cggatggtgc cgcgcagcac
     2761 ggggtagcgc gcctccagcg cgtcgaggac ggcccgctgg gtcaccggct cggccaccgc
     2821 cacgctcacc tccgcgccaa cgccagccag attgcgcagg tggtggggga tgatgacgcg
     2881 gatcacggca ggagctgcgc ctcgaccgag agcacggccg gcagatcgtg gacgatggcc
     2941 tgccagtggt cgccgccgtc ggccgagacg tagacctggc cgccggtcgt gccgaagtag
     3001 atgccgcagg ggtcaagctt gtccacgctc atggcgtcgc gcaggatgtt gacgtagcag
     3061 ttctcttgcg gcaggccgtc ggtcagcgct tcccactcgt tgccgccgcg gcggcgatag
     3121 acgcgcagct tgccatcggg cgggtagtgc tccgagtcgc tcttgatggg cacgacgtag
     3181 atcgtctccg gctcgtgggc gtgcacgtcg atgacgaagc caaagtcgga cggcagactg
     3241 ccgctgatct cgtaccactc atcgccggca ttgtcgctgc gcatcacatc ccaatgcttc
     3301 tgcatgaaga gccggtcggg attcgatggg tgcatggcga cgtggtggac gcagtggccc
     3361 acctcggctt tggggtcggg tatgtagtcg gagcgcagac cctggttgat ggccttccac
     3421 gtctgcccgc cgtcgtcgct gcggaacgcg ccggcggcgg agatggcgac gacgatgcgc
     3481 tcggggttcg acgggtcgag gaggatagtg tgcaggcaca ggccgcccgc gcccggctgc
     3541 cagatgccgc cggtgtcgtg ctggcgcagg cgggg
//
LOCUS       DAOSMQ010000332         3572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26216_length_3572_cov_4.584873, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000332 DAOSMQ010000000
VERSION     DAOSMQ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26216_length_3572_cov_4.584873"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3441)
                     /locus_tag="PK829_10760"
     CDS             complement(<1..3441)
                     /locus_tag="PK829_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOU41735.1"
                     /translation="MNQKYRLSPSTIAAYFKHRCDRQYRWESVLQSDRGRAGIGWNVP
                     RTPRFQSRPGITLLMAAGDEFEVSRLEELQQEHGPAAIAYAGIESDNGRRTVMPMPMP
                     ELAAVLRRPSPPRFVAQILIDLEATPDHAARFLRRFDLDPARVRVGTARPDLLAIIPG
                     DERRLLRVWDFKSSQAARHEHFIQVAFYTFLLEEALDAYGLSDAYAVDTSTGVIFARQ
                     GPDSFELAPYRLAIEDFLRHRAMPMLETPANEAHYHVNVGCPMCQYIDTCRAEADAGR
                     DLSRIAYMTSESKRRLARAGFHTHRELAQLSEDDPRWEQLRAAGHDLSMNLGRYVAVA
                     QALDDGRPRPLGSRLLTVPAWEDVRVTLSAEYDPVTNTCFALGLKTLAGWQAGRPQGM
                     EEVFLLENPLTTPDAEAQLLLRFLRVLNALLLRVDADNAAVAAEMDAEPALAAARRAA
                     YDAASAYAAFKAANPTLRRNKNNGDLIARRDALQAAEKETKRALQDAEREARYQNARR
                     QQRLHFYAFDGFDLTVLKRLVERHLFTTDAALRAELHTLVRLFPPESVMPDADSFRTI
                     PGTVVIDALRQLVALPSPYVYDLRSVTELIPAANGRRPYAFRPPSNFVYRGTNQIAFE
                     RIHDIWHARHFQPNPRDPASAIPPEEVRRRIERAVRDKLRATDEVIQWLKRDYRDGLL
                     LRKEPFRLHDGFNPLGFGMLDALRTFTMLETSYAELSVKHLHTLPIADRVARFECLSG
                     LRYIEAADEPDGSLWFTFDPAARDAKFARGDFDLVLTPAATPEQLVSHIDDPLFDRPR
                     WRHEPFKVSLVEYDLQANPPRVRLKPNLPLEKFREKVDLTAVQTLDRVYVDYNTSKTI
                     AVLNRLGTAPEIGQHVHELLDWASTSTWRPLIDDVGAVERELAACAARSGGSLSLTSA
                     QTRAFRGAPAVPLSLIWGPPGTGKTTMLAHLLLAYVLAARRGGRPVRILVSAFTHNAI
                     NNVLNKVAELLGRYGLGGSATLVKVQGNQENAQDGQLPAAVERAHNKALAGLLGSDAP
                     CVIAGSTVWGIYNAMNSAGGPVQPWFDVVLIDEASQMKVAEALIAFGAARPDGAVILA
                     GDDRQLPPIIHGDYPAEHREMLSSVFAFMRARIEAHEEDGFAARTLFQLEENFRGNEA
                     L"
BASE COUNT          591 a         1099 c         1293 g          589 t
ORIGIN      
        1 cagggcttcg ttaccgcgga agttctcctc caactggaag agcgtgcgcg ccgcgaagcc
       61 gtcttcctcg tgcgcctcaa tgcgggcgcg catgaaggcg aagaccgacg acagcatctc
      121 gcggtgctcg gccggatagt cgccgtggat gatgggtggc aactggcggt cgtcgccggc
      181 caggatgacc gcgccatcgg gccgcgcggc gccgaaggcg atcagggcct cggccacctt
      241 catctgcgag gcctcatcga tgaggacgac gtcgaaccag ggttgcaccg gcccaccggc
      301 gctattcatg gcattgtaga tgccccagac ggtgctgccg gcgatgacac agggcgcgtc
      361 gctgcccagc aggccggcaa gggccttgtt gtgggcgcgt tcgacggccg cgggcaattg
      421 cccgtcctgg gcgttctcct gattgccttg caccttgacc agcgtcgcgc tgccgcccag
      481 gccatagcgc cccagcagtt cggcgacctt gttgaggaca ttgttgatgg cgttgtgggt
      541 gaaggcgctg acgagaatgc gcaccggccg gccgccgcgg cgagcggcca gcacgtaggc
      601 cagcagcaag tgggccagca tggtcgtctt gcccgtgccc ggcggccccc agatgaggct
      661 gaggggcaca gccggcgcgc cgcggaaggc gcgcgtctga gccgaggtca gagagagact
      721 tcctccgctg cgggcggcgc aggccgccag ttcccgctcg accgcgccaa cgtcgtcgat
      781 gagcggccgc cacgtggacg tactcgccca gtctagcagt tcgtgaacgt gttggcctat
      841 ctccggcgca gtccccaggc ggttgaggac agcgatggtc ttggaggtgt tgtagtccac
      901 gtagaccctg tccagcgtct ggacggcggt caggtcgact ttctcgcgga acttctccag
      961 cggcaggttg ggtttcagcc ggacgcgcgg tggattggcc tgcaagtcgt attcgaccag
     1021 actgaccttg aatggctcat gccgccagcg aggacggtcg aagagggggt cgtcgatgtg
     1081 gctgaccaac tgctccggcg tcgccgccgg ggtcagcacc aggtcaaagt cgccgcgggc
     1141 gaacttggcg tcgcgggcgg ccgggtcgaa ggtgaaccac agcgagccgt ccggctcgtc
     1201 ggctgcctca atgtagcgca ggccgctgag acactcgaac cgggccaccc gatcggcgat
     1261 gggcagcgtg tgcaagtgct tgacggacag ctcggcatag gaagtctcca gcatggtgaa
     1321 ggtacgcagc gcgtccagca tcccgaagcc gagcgggttg aagccgtcgt ggaggcggaa
     1381 gggctccttg cgcagtagca ggccatccct gtagtcgcgc ttcagccatt ggatgacctc
     1441 atccgttgcc cgcagcttgt cgcgcacggc ccgctcgatg cggcggcgca cttcctccgg
     1501 cgggatggcg ctggccggat cgcgcgggtt gggctggaag tggcgggcgt gccagatgtc
     1561 gtggatgcgc tcgaaggcga tctggttggt gccacggtag acgaagttgg atggcggccg
     1621 gaaagcatag gggcgccgcc cgttcgcggc tggaatcagt tcggtcacgc tacgcagatc
     1681 atagacatag ggcgacggca aggccaccag ctgccgcagc gcgtcgatga cgaccgtgcc
     1741 ggggatggtg cggaagctat cggcgtcggg catgaccgac tccggcggga agagacgcac
     1801 cagggtatgc agttcggccc gtagcgcggc gtcggtggtg aacaggtggc gctccaccaa
     1861 ccgcttgagc acagtcaggt cgaagccgtc gaaggcgtag aagtgcagcc gctgttgccg
     1921 ccgggcgttc tgatagcggg cttcgcgctc agcgtcctgg agcgcgcgct tggtttcctt
     1981 ctcggccgcc tggagcgcgt cgcgccgggc aatgaggtcg ccgttgttct tgttcctgcg
     2041 cagggtgggg ttggccgctt tgaaggccgc gtaggcgctg gctgcgtcgt aggcggcgcg
     2101 gcgcgccgcg gccagggccg gctccgcgtc catctccgcg gccacggcgg cgttgtccgc
     2161 gtccacgcgc agcagcaaag cgttgagcac gcgcagaaag cgcagcagca attgcgcctc
     2221 ggcgtcgggc gtcgtcaggg ggttctccag caggaaaacc tcttccatgc cctgcggccg
     2281 cccggcctgc cagcccgcca gcgtcttgag gcccagggca aagcaggtgt tggtcaccgg
     2341 atcgtactcg gcgctgaggg tcacgcggac gtcttcccag gcgggtacag tcagcaggcg
     2401 actgcccagc gggcgcggcc ggccgtcgtc cagcgcctgg gccacggcca cgtagcgccc
     2461 caggttcatt gacaggtcgt ggccggcggc gcgcagttgc tcccagcgcg ggtcgtcctc
     2521 gctcaactgg gccagttcgc ggtgggtgtg gaagcccgcc cgcgccagcc gccgcttcga
     2581 ctctgaggtc atgtaggcga tgcgcgacag atcgcgcccg gcgtcggcct cggcgcggca
     2641 ggtgtcaatg tactggcaca tggggcagcc cacgttgacg tggtagtggg cctcgttggc
     2701 gggcgtctcc agcataggca tggcccggtg gcgcagaaag tcctcaatgg ccaggcgata
     2761 gggggccagc tcgaacgagt ctggcccttg ccgggcaaag atgacgccgg tgctggtatc
     2821 gacagcgtag gcatcgctca gaccataggc gtcgagggct tcctccagca ggaaggtgta
     2881 gaaggccacc tggatgaagt gctcgtggcg ggcagcctga ctggacttga agtcccaaac
     2941 gcgcaataaa cggcgctcgt cgccagggat gatcgccagc agatcgggcc gggccgtgcc
     3001 gacgcggacg cgcgccgggt caaggtcgaa gcgccgcagg aagcgcgcgg cgtggtcggg
     3061 cgtggcttcc aggtcgatga gaatctgcgc gacaaagcgc ggcggcgacg gccggcgcaa
     3121 cacagcggcc agttcgggca tgggcatggg catgacggtg cggcggccgt tgtcgctctc
     3181 aatgccggcg taggctatcg ccgccgggcc atgctcctgt tgcaactcct ccaaccggct
     3241 gacctcgaac tcgtcgccag cggccatgag cagagtgata ccggggcggc tctggaagcg
     3301 cggcgtgcgg ggtacgttcc agccgatgcc agcccggccg cgatcagatt gcaggacact
     3361 ttcccagcgg tactggcggt cgcagcggtg tttgaagtag gcggcgatgg tggaggggct
     3421 taggcgatac ttttggttca tgacgcgccg ctagtttatc gtggatggca gagaatgaaa
     3481 ccacagatca cgccgatttc acggattgaa aagaggctca cgcaaaggcg ctaagcaaga
     3541 aaaggcgcaa aggaagaaat agataaactc tt
//
LOCUS       DAOSMQ010000333         3567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26266_length_3567_cov_5.138098, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000333 DAOSMQ010000000
VERSION     DAOSMQ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26266_length_3567_cov_5.138098"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..2107)
                     /locus_tag="PK829_10765"
     CDS             complement(11..2107)
                     /locus_tag="PK829_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41736.1"
                     /translation="MTPMTLTPVQRDAVAAGGTSYLYGPAGTGKTTALQQRLLRLLRE
                     GEPAYTILVLVAEPEHRDAFFDTLHTSGLGPYAELNVTHYTRLAQSMVSLFWPLVARG
                     AGFARPYQPPTFLSYDLAQVLMWRVVNPLLGQGAFANLRLRPQQIVSQLLDTLNRAAL
                     NALTLEEAIARQARTWAGDPERRFHLDDAATAARAFRRRCLANSLLDLSLTVEVFDTQ
                     LVQHPEFHRYFRERYRHLLVDNLEEQTPAGQNFVVGLMGETQTTAVAGDAGGGYKRFL
                     AADPHGAEALRARFDHVYDFTTSFVAPPEMSQLADVVENALLRTRHPVPDAGADGQLL
                     GVIGGRYRREMVNNLAPVLHSLVYDQDVAPRDIAIIVPYLDGALRYMLTQALAEAGLP
                     YRLLRRRTSPREEPRVRAWLTWLALTHPEWGVHPAPFDVAEALTLSIASLDPARAQLL
                     VDNLYRPDVPELAPAEGLPERIVARVGAENVELAEELRLWLAARDRGESIDAFLHSLF
                     NELLSQPRFRPEPDLAGAAVADWLSQAAARLRRAGTAMGLAGEAEAGAAFIDAVNQGL
                     VTANPPDWGEPPDPNGIVLATIYAYLLAGPPARVQVWLETAAQGWWDIPRQPLSNAFV
                     LVQSRAPEEPWTIEEDFAVRNELLARIVRGLTARCRDGIILASSDLDRRGQRQDGPLW
                     RALAPLVHRFSVVTNS"
     gene            complement(2264..>3567)
                     /locus_tag="PK829_10770"
     CDS             complement(2264..>3567)
                     /locus_tag="PK829_10770"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU41737.1"
                     /translation="RPEMAAAIVLISDGTDPGTSQVQPSDVPTRAAAAGIPIHTIVID
                     NAGLNIGQELGREYMRDVATGSRGLTTDLSNAEGLAAIWGRIAGFRDHYLIRYTAPEV
                     ASGTFPVEVSLLNNRDTRAVADVTISTAAPSVVIDLPRESRAITVTNLDKPIELRLAT
                     TVSWLDGQTRDVTAARLLVNDVAAGDIPVDDLSGFTMPIGNLSFGDNRLEVEVTDSQG
                     LTSKSAPVILSVTQGEEEVLPEDLQPSGSGFNIWWVAIPLLLLATAGLGYYLWQQSQR
                     PRRGLAAAEAPAGNRRRRRGAAPEVVPSPPPPAAMGGYDAPGDMGYMGGSAGEQPFIM
                     AHLEVINAQTMMPEELTLGDLEVRVGRSPAQSQIAFRDDITVSRFHAVLRLEGNHYRI
                     YDAGSTSGTYVNGRQAPEYGLQLSDGDEIQLGAVRLRYRQL"
BASE COUNT          531 a         1158 c         1318 g          560 t
ORIGIN      
        1 cggtcaggtc tcaagaattg gtgacaactg aaaagcggtg gacgagcggc gcgagcgctc
       61 gccacaatgg tccgtcctgc cgctggccgc gccggtcgag gtcgctgctg gccaggataa
      121 tgccgtcgcg gcagcgcgcc gtcagcccgc ggacgatgcg cgccagcagt tcgttgcgca
      181 cggcgaaatc ctcttcgatg gtccacggct cctccggggc gcggctctgc accaggacaa
      241 aggcgttgct gagcggctgg cgggggatgt cccaccagcc ctgggcagct gtctccagcc
      301 agacctgcac gcgcgccggc ggcccggcca gcaggtaggc gtagatggtc gccaggacga
      361 taccgttggg gtcgggcggc tcgccccagt cgggcgggtt ggccgtcacg aggccctggt
      421 tgacggcgtc gatgaaggcc gcgccggcct cggcctcgcc ggccagcccc atcgccgtcc
      481 cggcgcgccg caaccgggcc gcagcctggc tcagccagtc ggccacggcc gcgccggcca
      541 ggtctggctc cggccggaag cgcggctgcg agagcagttc gttgaagagg ctgtgcagaa
      601 aggcgtcgat gctctcgccc cggtcgcgcg cggccagcca caggcgcagc tcctcggcca
      661 gttcgacgtt ctccgcgccg acgcgggcga cgatgcgctc cggcaggccc tcggccgggg
      721 ccagttcggg cacgtcgggc cggtagaggt tgtccaccag cagttgcgcc cgcgccgggt
      781 cgaggctggc aatgctcagc gtcagcgcct cggccacgtc gaagggcgcg ggatggacgc
      841 cccactccgg gtgcgtcagg gccagccacg tcagccaggc gcgcacgcgc ggctcctcgc
      901 gcgggctggt gcggcggcgc agcaggcgat agggcagccc ggcctcagcc agcgcctggg
      961 tcagcatgta gcgcagcgcg ccgtccaggt aggggacgat gatagcgatg tcgcgcgggg
     1021 ccacgtcctg gtcgtagaca aggctgtgta gcaccggggc gaggttgttg accatctcgc
     1081 gccggtagcg cccgccgatg acgcccaaca gctgcccgtc cgcgcccgcg tctggaacgg
     1141 gatggcgtgt gcgcagcaga gcgttctcga cgacgtcggc cagttggctc atctccgggg
     1201 gggcaacgaa gctggtggtg aagtcgtaga cgtggtcgaa gcgggcgcgc aatgcctccg
     1261 cgccgtgggg gtcggcggcc aggaagcgct tgtagccgcc gccggcgtcg ccagccacgg
     1321 ccgtcgtctg cgtctcgccc atcaggccga cgacgaagtt ttgccccgcg ggcgtctgct
     1381 cctccagatt atcgaccagc aaatggcggt agcgctcgcg gaagtagcgg tggaattcgg
     1441 ggtgttgcac cagttgggtg tcgaacactt caaccgtcag cgacagatcg aggaggctgt
     1501 tggccagaca gcggcggcgg aaagcgcgcg cggccgtagc ggcgtcgtcg aggtggaagc
     1561 ggcgctccgg gtcgccggcc caggtgcggg cctggcgggc gatggcctcc tccagggtca
     1621 gggcgttaag ggccgcccgg ttgagcgtgt ccagtagctg gctgacgatc tgctgcggcc
     1681 gcaggcgcag gttggcaaac gcgccctggc ccaacagcgg gttgaccacg cgccacatca
     1741 gcacctgagc caggtcgtag ctcagaaagg tcggcggctg gtagggccgg gcgaagcccg
     1801 ccccgcgggc caccagcggc cagaagagcg acaccatgct ctgggccagc cgcgtgtagt
     1861 gggtcacgtt tagctcggca taggggccga gaccagaggt gtggagcgtg tcgaagaagg
     1921 cgtcgcgatg ctccggctca gccaccagga cgaggatggt ataggccggc tcgccctcgc
     1981 gcaggaggcg caggaggcgc tgctggagcg cggtcgtttt gcccgtgccg gccgggccgt
     2041 agaggtagct cgtcccaccg gcggcgacgg cgtcgcgctg aacaggggtg agggtcattg
     2101 gcgtcatggg ccaattatac gacggaaggt ggccgcggtc gtattggccc cgcccttata
     2161 cagggctttt cccaaagtcg tcagttgctc gaacacctga caggtgggcg gccacctgtc
     2221 aggtcagcgc actttttagc gactttggaa aagacctgcg cccttatagc tgtcgatagc
     2281 gcagtcgcac cgcgccaagt tgaatttcgt cgccgtcgct cagttgcagg ccatactccg
     2341 gcgcttgccg gccgttgacg taggtgccgc tggtgctgcc ggcgtcgtag atgcggtagt
     2401 ggttgccctc cagccgcagg acggcatgga agcgggagac ggtgatgtcg tcgcggaagg
     2461 cgatctggct ctgggccgga gatcggccga cgcgcacttc caggtcgccc aatgtcagct
     2521 cctccggcat catcgtctgc gcgttgatga cttccagatg ggccatgata aacggctgtt
     2581 cccccgcgct gccacccata tagcccatgt cgccgggcgc gtcatagccg cccatcgcgg
     2641 ccggcggcgg cggtgatggc acaacctctg gcgccgcgcc gcggcggcgg cggcggttgc
     2701 cggcgggcgc ttcggccgcg gccaggccgc gccgcgggcg ctggctctgc tgccacaggt
     2761 agtagcccag cccggcggtg gccagtagca gcaggggaat ggcgacccac cagatgttga
     2821 agcccgaccc cgacggctgc aagtcctctg gcaacacctc ttcctcgccc tgggtcacgc
     2881 tcaggatgac cggcgcgctc ttggaggtca ggccctggct gtcggtgact tcgacctcca
     2941 gccggttgtc gccgaagctg aggttgccga tgggcatggt gaaaccgctc aggtcgtcta
     3001 cggggatgtc gccggcggca acgtcgttga ccagcaaccg ggcggcggtc acgtcgcgcg
     3061 tctggccgtc gagccaggac acggttgtcg ccaggcgcag ctcaataggc ttgtcgaggt
     3121 tggtcacagt gatggcccgg ctctcgcggg gcaggtcgat gacgacgctg ggcgcggccg
     3181 tggaaatggt cacgtcggcc acggcgcgcg tgtcgcgatt gttgagcaga ctgacctcga
     3241 ccgggaaggt gccgctggcg acttcgggcg cggtgtaacg gatcaggtaa tgatcgcgga
     3301 agccggcgat gcggccccag atggcggcca gtccctcggc gttggacagg tcagtggtta
     3361 gcccgcgcga gccggtggcc acgtcgcgca tatactcgcg ccccaactcc tggccgatgt
     3421 tcagcccggc gttgtcgatg acgatggtgt ggatggggat gccggccgca gccgcccgcg
     3481 tagggacatc gctcggctga acctgcgacg taccggggtc ggtgccgtcg ctgatgagga
     3541 cgatggccgc ggccatctcc ggccgcg
//
LOCUS       DAOSMQ010000334         3553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26398_length_3553_cov_4.265866, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000334 DAOSMQ010000000
VERSION     DAOSMQ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26398_length_3553_cov_4.265866"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK829_10775"
     CDS             <1..320
                     /locus_tag="PK829_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU41738.1"
                     /translation="QAAEVIRETTHPDANVIFGAVIDENMGDEVRITVIATNFDQAHL
                     PRHMLQSSDSGLFETAARQVRSAERQSKPPQPASRPEAPAVEEPARFMPGNLEVPAFL
                     RRK"
     gene            complement(471..2120)
                     /locus_tag="PK829_10780"
     CDS             complement(471..2120)
                     /locus_tag="PK829_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU41739.1"
                     /translation="MRDRTIVRLGLLILALTTLACNAFAGVARPTLPPPPLVVPSPAT
                     TGDAAVPDVAPTATLPGETPAGASQGTLRVLVDLNVRAGPGVGYERVGFLLRDETAVI
                     LGRDPASGWWKIACPPRAEGNDCWVSGGAQYTQAENAAAAPTAAVPASPTPRPPDPAP
                     GTGLLVFIDSGRLFASTLDLTGDVPTAAPALQLGTAASVQRAAIAPDGRTVAFTVLDP
                     TTGDNELRLIHADGANEHTLVRAAELPRVPEATELPAAATATASARAQVLDFQWQSDG
                     AALVFNTALVSPGGFSAGSQSDLWTVTPQGQVTARLAAGRGLPRFVVSPDDRLLLIGR
                     QEILRVAIDGRGLESVLTFEPVATAENVIYPTVQWSVDGTFALAAVTAPFAAGDATET
                     RSAGLWRVPGAGAAVALGQVRADVVAASPRWSPDGQRLAYLRASAAGPELWLANGDGT
                     RLERVAAGEGLRLLDWRAGNNALLFAGQDYAAVVQSGAPAIIAQTGGAVGRGAWLSDT
                     AVVLALTDGSVTGFFVVTRDGTRQLLAPLSAVEAPLDVWLP"
     gene            2365..>3553
                     /locus_tag="PK829_10785"
     CDS             2365..>3553
                     /locus_tag="PK829_10785"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOU41740.1"
                     /translation="MSFRHFIEKADKAGYLLTIDRPVDTTFEIANVAHALEGRPVLFN
                     HIDGYPGWRVCAGPCADRAFFSLALDVPVPALMRHLADALAQPVAPPLVDSAPCQEVV
                     VEEVDLDQLPILLHLPQDAGHYIASAVVIVEDPDLGRNMCYHRLLRLDKRHFAARIVE
                     NRGTYTALQKSSGDLPVAICIGVSLAVHLAASMSPPPGVDELAVAHALAPTPLVKCLT
                     NDLSVPADAEIVLEGRISHQLVSEGPFMDLTETMDITRQQPVIAIDLITHRRDPIYHA
                     LLPGGLEHKLLMGMPKEPTIFAAVNEVARCTGAVITPGGTSWLHAVVQIDKQGEDDGR
                     RAIEAAFRGHGSLKHVWVVDTDVDIYDPAQVEWAMATRFQADRDLYVYPNQPGSSLDP
                     SGTH"
BASE COUNT          566 a         1300 c         1151 g          536 t
ORIGIN      
        1 atcaggccgc cgaggttatc cgcgaaacca cccaccccga tgccaacgtc atcttcggcg
       61 cggtcatcga cgaaaacatg ggcgacgagg tgcgcatcac cgtcatcgcc acgaacttcg
      121 accaggccca tctgccccgg cacatgctgc aaagcagcga ctccggtctc tttgagacgg
      181 ccgcccgtca ggtgcgtagc gccgaacggc agagcaagcc gccccagccg gcttcgcgcc
      241 ctgaggcgcc ggccgtggaa gagccggcgc ggttcatgcc cggcaatttg gaagtcccgg
      301 cctttctgcg ccggaaatag gcacgcttta ccgaccggcc ggcgttcctc tcgcccggcc
      361 gctaacatag cagtctcgca ggctacatcc cctctctgaa gctccccctg tttcgcagaa
      421 cagggggagc gcccttttct caagggccat ccccctcaga gccggcttat tcagggcaac
      481 cacacgtcca gcggcgcttc cacggccgac agaggcgcca acaattggcg ggttccgtcg
      541 cgcgtcacga caaagaagcc cgtcacgctg ccgtcggtca gcgctaagac gacggccgtg
      601 tcgctcagcc acgcgccgcg gccgaccgcc ccgccggttt gggcgatgat cgccggcgcg
      661 ccactctgaa caacagcagc atagtcctgc ccggcaaaga gcagcgcgtt gttgccggcg
      721 cgccagtcca acaggcgcag tccctcgccg gcggccacac gctccagccg cgtgccgtcg
      781 ccattggcca gccacagttc cggaccggcg gccgacgcgc gcagataggc cagccgctgg
      841 ccatccggcg accaacgcgg cgacgcggcg acgacatcgg cgcgcacctg gcccagggcc
      901 acagccgccc cagcgccggg aacgcgccac aggccagccg agcgcgtctc ggtcgcgtcc
      961 ccggcggcga acggcgccgt cacagccgct agggcgaacg ttccatcgac gctccattgc
     1021 accgtcggat agatgacgtt ctcagccgtg gccaccggct caaaagtcag caccgactcc
     1081 agcccgcggc cgtcgatggc cacgcgcagg atttcctgcc ggccgatgag cagcagccgg
     1141 tcgtccgggg agacgacgaa gcgcggtagc cctcgccccg cggccagacg cgccgttacc
     1201 tggccctggg gtgtcactgt ccacaggtcg ctctgcgaac cggccgagaa gccgccgggg
     1261 ctgaccagag ccgtattgaa gaccagcgcc gccccgtcgc tctgccactg gaagtccagc
     1321 acctgcgcgc gggcactagc cgtggccgtg gccgcggccg gcagctcggt ggcctccggc
     1381 acgcgcggca gttcggcggc gcgcaccagg gtatgttcgt tggccccatc ggcgtggatg
     1441 agccgcagtt cgttgtcgcc cgtcgtcgga tcgaggacgg taaaagccac cgtgcggccg
     1501 tccggggcga tggccgcccg ctgcaccgag gccgccgtgc ccagttgcag cgccggggcg
     1561 gccgtgggca cgtcgccggt cagatcgagc gtgctggcga agaggcggcc gctgtcgatg
     1621 aaaaccagta gcccggtgcc gggcgcgggg tcgggcggcc ggggcgtcgg cgaggcgggg
     1681 acagcggcag tgggggctgc ggcggcattc tcggcctgtg tatattgcgc gccgccagac
     1741 acccagcagt cgttgccctc ggcgcggggt gggcaggcga tcttccacca gccgctggcc
     1801 ggatcgcgcc ccaggatgac ggcggtctca tcacgcagca ggaagccgac gcgctcatag
     1861 cccacgcccg gcccggcgcg gacattcaga tcgaccagaa cccgtagcgt gccctggctc
     1921 gccccggcgg gggtctcgcc cggcagggtc gccgtcgggg cgacgtcggg gacggccgcg
     1981 tcgcccgtcg tcgccggcga cggcacgacc agcggcggcg gcggcagcgt cggccgggcg
     2041 acgccggcga aggcattgca ggccagcgtc gtcagcgcca gtatgagcag tcccaggcgg
     2101 acaatcgtcc gatcgcgcat cgtgtccttt gtgtcggggc catgccggcc cgacaggcga
     2161 cgctcattat agcgcaggaa ccgtccacgt ccttgccggg cttttcggga gacggccgtg
     2221 gcgtccagag atgacagtca ggcggctaca agtttcgttc ctgtcagagc cgtattggcc
     2281 gtcaacctgt caggtcagtg gccagccgtc gcctgccggc cgtccctccg ctataataca
     2341 ctgccgagga aggaaacgac gtccatgagc tttcgccact tcattgagaa ggccgacaag
     2401 gccggctatc tgctgaccat cgaccggccg gtcgatacga ctttcgagat cgctaacgtt
     2461 gcccacgctc tggagggccg gccggtgctc ttcaaccaca tcgacggcta ccctggctgg
     2521 cgcgtctgcg ccgggccatg cgccgaccgg gccttcttca gcctggccct cgatgtgccc
     2581 gtgccggctc tgatgcgcca tctggccgac gcgctggccc aaccggtcgc tccgcccctg
     2641 gtcgacagcg ctccttgcca ggaggtcgtc gtcgaggaag tcgatctcga ccaactgccc
     2701 atcctgctcc atctgccgca ggacgccggc cactacatcg ccagtgctgt ggtcatcgtc
     2761 gaagaccccg acctcggccg caacatgtgc taccatcgcc tgctgcgcct tgacaagcgc
     2821 catttcgccg cccgcattgt cgagaaccgc ggcacctaca cggccttgca gaaaagctcc
     2881 ggcgacctgc ccgtggccat ctgcattggc gtctcgctgg ccgtccatct ggccgcgtcg
     2941 atgtcgccgc cgccgggcgt cgatgagttg gccgtggccc atgccctcgc gcccacgccg
     3001 ctggtcaagt gcctgaccaa cgacctgtct gtgcccgccg acgcggagat cgtcctggag
     3061 gggcgcatta gccaccagct cgtgtccgaa gggccgttta tggatctgac cgagacgatg
     3121 gacatcactc gccagcagcc ggtcatcgcc atcgacctga tcacccaccg gcgcgacccg
     3181 atttaccacg cccttctgcc cggcggtctg gagcacaagc tgctgatggg tatgcccaag
     3241 gagccgacta tcttcgccgc tgtcaacgag gtcgcccgct gcaccggcgc ggtcatcacc
     3301 cccggcggca cgtcctggct ccatgctgtc gtccagatcg acaagcaggg cgaagacgac
     3361 ggccgccggg ccattgaggc cgccttccgc ggccacggct cgcttaagca cgtctgggtg
     3421 gtcgatacag acgtggacat ctacgacccg gcgcaggtcg aatgggccat ggccacgcgc
     3481 ttccaggccg accgcgacct gtatgtctat cccaaccagc ccggcagctc gctcgacccg
     3541 tccggcacgc acg
//
LOCUS       DAOSMQ010000335         3552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26408_length_3552_cov_5.131827, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000335 DAOSMQ010000000
VERSION     DAOSMQ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26408_length_3552_cov_5.131827"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..2478)
                     /locus_tag="PK829_10790"
     CDS             complement(64..2478)
                     /locus_tag="PK829_10790"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOU41741.1"
                     /translation="MKKSLLALVLFVWLAALVAPGAQAQDQIPPRATEALVPPIDPPI
                     CLDLCPPPVWNMDGLEIAYQRVNVTIADQVATTHIEQLFRNPNDWALEGTYYFPLPAG
                     ASVSQLTMWVNGAPIEARLMPAAEARAVYDEIVRQLRDPALLEYVGQDAIQANVFPIP
                     PGEERLVEIAYTTLLTADNGTFHYVYPQSAGLYTDTPLTQQSIRVDIASNEAIRTLYS
                     PSHRVAIARDGDFRATVGYEAANPPLDDFELYYTVSPEAIGLNLLSYKAAGEDGFFVL
                     LVAPGLDAGEVVAKDIILVLDTSGSMEGEKIIQARGAARYVVDHLNPDDRFAVVQFNT
                     TVSSYAPALLPTSEVAGAGAYIDTLPAAGGTNISGALLEAAALVQPERPTTILFLTDG
                     LATEGITDTGLLLDNVAAALPDNARLFAFGVGDDVDTTLLDTLAQNHRGTTTYVRPGQ
                     AIDESVSAFYARVSAPVLTDIAIDYGGVAVQEVYPVELPDLFAGTQLVIAGRYRDGGP
                     ATITLSGTTNGRAQRFSYPDQTFQADGGEAFIPRLWATRAIGHLMQQIRLHGEDPELV
                     QSVVALSTRYGIITPYTSFLIEEDDISAQSPDVFAAPLAAPAEASGAEAVERAAAEGD
                     MAAAEAPLPLPTLMPSAGAAPGMAVAEAVAPVQTAGDRTFFLRDGVWVDSAYDAAMGE
                     PAVVPFASDAYFDLLSARPELGAALALGDEVLVMVEGVAYRITATSGEAGEVTLPGTA
                     STPTAAPDGITQTDATPAAINATPGATTEGTSGVTAFGVGPCAAALLLPLAIVVSLGA
                     ARRRRD"
     gene            2598..2879
                     /locus_tag="PK829_10795"
     CDS             2598..2879
                     /locus_tag="PK829_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002752639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOU41742.1"
                     /translation="MKVQWDERKAAGNKQKHGVSFEEAATVFSNPLALIFDDEWHSEA
                     EEREIIVGHSTGGELLVISFTEREGAVRIISARPATAREQSDYEEDTFR"
     gene            2857..3072
                     /locus_tag="PK829_10800"
     CDS             2857..3072
                     /locus_tag="PK829_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41743.1"
                     /translation="MKKTRSDKGDELDDLLPEYEFDYKKAKPNRFALTEQQRVVILEP
                     DVAEYFRDSEAVNRVLRALIEVMPREV"
     gene            3314..>3552
                     /locus_tag="PK829_10805"
     CDS             3314..>3552
                     /locus_tag="PK829_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41744.1"
                     /translation="MQLPPLAQARVGIVGLGLMGGSLALALRGRVAHLLAVEPQVVAR
                     QMALREGVVAEAAEALTPGTPPLDLGLHPFRRHFE"
BASE COUNT          604 a         1093 c         1238 g          617 t
ORIGIN      
        1 acctgtcagg tcggtgcgcc tggggtgact ttggaaaagc cctagatagg gtaggcccct
       61 gcgctagtcg cgccggcggc gggccgcgcc caggctgacg acgatggcca gcggcagcag
      121 cagggccgcg gcgcacgggc caacgccaaa ggccgtgacg ccgcttgtgc cctctgtggt
      181 cgcgccgggc gtggcgttga tggccgccgg ggtggcgtcc gtctgagtga tgccatcggg
      241 cgcggccgtc ggcgtgctgg ccgtacccgg cagggtaact tcgcccgcct cgccgctcgt
      301 cgcggtaatg cgataggcca cgccctcgac catgaccagc acctcgtcgc ccaacgccag
      361 cgccgcgccc agttccggcc gggcgctcag caggtcgaag taggcgtcgg aggcgaaggg
      421 gacgaccgct ggctcgccca tcgcggcgtc ataggcgcta tcgacccaca cgccgtcgcg
      481 cagaaagaac gtccggtcgc cggccgtctg caccggcgct accgcttcgg ccacggccat
      541 gcccggcgcg gccccggcgc tgggcatcag cgtcggcaag ggcaggggcg cttcggccgc
      601 ggccatatcg ccctcagccg ccgcccgctc gactgcttcc gcgccactgg cctcggccgg
      661 cgcggccagc ggcgccgcga acacgtccgg cgactgggcg ctgatgtcat cctcctcgat
      721 cagaaagctg gtgtagggcg tgatgatacc gtagcgcgtg ctcagcgcga cgacgctctg
      781 cactagctcc gggtcttcgc cgtgcaggcg aatctgctgc atcaggtggc cgatggcccg
      841 cgtggcccac aggcggggga tgaacgcctc gccgccatcg gcctggaaag tctggtcggg
      901 gtaactgaag cgctgcgccc ggccgttggt cgtgccgctc agagtgatcg tcgccgggcc
      961 gccgtcgcgg tagcggccgg caatgaccag ttgcgtcccg gcgaagaggt cgggcagttc
     1021 cacggggtag acctcttgca cggccacgcc gccgtagtcg atggcgatgt cggtcagcac
     1081 cggggcgctg acgcgggcgt agaaggcgct gacagactca tcgatggcct ggccggggcg
     1141 gacgtaggtc gttgtcccgc ggtggttctg ggccagcgtg tccagcagcg tcgtgtccac
     1201 gtcgtcgccg acgccgaagg cgaacaggcg ggcgttgtcg ggcagcgccg cggcgacgtt
     1261 gtccagcagc aggccggtgt cggtgatgcc ctcggtggcc aggccgtcag tcaggaagag
     1321 gatggtcgtc ggccgctccg gctgcaccag cgcggcggcc tccagcagcg cgccgctgat
     1381 gttcgtgccg ccggcggcgg gcagcgtgtc gatgtaggcc ccggctccgg caacctcgct
     1441 cgtgggcagg agggccggcg cgtagctgct gaccgtggtg ttgaattgca ccacggcaaa
     1501 gcggtcgtcg gggttgagat gatcgacgac gtagcgggct gccccgcgcg cctggatgat
     1561 cttctcgccc tccatgctgc ccgacgtgtc tagcaccagg ataatgtcct tggccacgac
     1621 ctcgcccgca tccagccccg gcgcgaccag caggacgaag aagccatctt cgccggcggc
     1681 cttatagctg agcaggttca ggccgatcgc ctcgggcgag acggtgtagt acaactcgaa
     1741 gtcgtccagc ggcgggttgg cggcctcata gccgaccgtg gcgcggaagt caccgtcgcg
     1801 ggcgatggcc acgcggtggc tgggcgagta gagggtgcgg atggcctcgt tggaggcgat
     1861 gtccacacgg atgctctgct gcgtcagggg cgtgtcggtg tagagaccag ccgactgtgg
     1921 atagacgtag tggaacgtgc cattgtcggc cgtcagcagt gtcgtgtacg cgatctcgac
     1981 cagccgctcc tcgcctggcg ggatggggaa gacgttggcc tggatggcat cctgaccgac
     2041 gtactccagc agggcggggt cgcgcaactg gcggacgatc tcgtcgtaga cggcgcgggc
     2101 ctcggcggcg ggcatcagcc tggcttcgat gggcgcgccg ttgacccaca tggtcagttg
     2161 gctgacggac gcgccggccg gcagcgggaa gtaatacgtg ccctccagcg cccagtcgtt
     2221 ggggttgcgg aagagctgct cgatgtgggt cgtggccacc tgatcggcga tggtcacgtt
     2281 gacgcgctgg taggcaatct ccagcccgtc catgttccag acgggtggcg ggcagagatc
     2341 aaggcagatc ggcgggtcaa tgggcggaac cagcgcctcg gtggcgcgcg gtggaatctg
     2401 atcctgcgcc tgcgcccccg gcgcaaccag cgcggccagc caaacgaaca acaccagcgc
     2461 aagcaaactc ttcttcatgg cgttcctccg gaacggatgg gtctatcagg aagacgcgcg
     2521 ggggcggggg aaggttcccc aatgggtagc gttggtcgcg cgtgtacaaa tggccgggtg
     2581 caagttatac ttgagcgatg aaggtacagt gggatgagcg caaagcggct gggaacaaac
     2641 aaaaacacgg ggttagcttt gaagaagcag caactgtatt ttcaaacccc ttagccctca
     2701 tatttgatga cgagtggcac tcagaggccg aagaaaggga gatcatcgtt ggacattcaa
     2761 ccggtgggga gcttctggta ataagcttca ccgagcgaga aggtgcagtt cgaatcatta
     2821 gcgcccgccc ggcgactgcg agagagcaaa gtgactatga agaagacacg ttcagataaa
     2881 ggcgatgaac ttgacgacct cttgccagag tatgaattcg attacaagaa ggccaagcca
     2941 aaccgattcg cgctgacgga gcagcagcgc gttgtgatct tggaacccga tgtagccgag
     3001 tatttccgcg attcggaggc ggtgaatcgc gtgctaagag cgttgattga ggtgatgccg
     3061 agggaagtgt gactgatttc gacagttgtc tattataagc tgtatccggt cgtgccaata
     3121 acagatggcg tgagtgcgga ggaattcaca tgccccagat aatagagacc ttccagcaac
     3181 aactgatgtg atcctagtaa tatttgttgc gttccttgcc ttaattggcg tggtcttcac
     3241 cggggtacga atgagctgcc cactccccca atccccatca tccgcgtccc cttcttccgc
     3301 tataatttct cccatgcagc tacctcctct ggcgcaagcg cgagttggca tcgtcggcct
     3361 ggggctgatg ggcggctcgt tggccctggc attgcgcggc cgcgtcgccc acctgttggc
     3421 cgttgagccg caggtcgttg cccggcagat ggcgttgcgc gagggcgtcg tcgctgaggc
     3481 cgccgaggcg ctgacgcccg gcacgccgcc gctggacctg ggcctgcacc ccttccgccg
     3541 ccacttcgaa ga
//
LOCUS       DAOSMQ010000336         3546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26468_length_3546_cov_5.046978, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000336 DAOSMQ010000000
VERSION     DAOSMQ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26468_length_3546_cov_5.046978"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PK829_10810"
     CDS             complement(<1..1515)
                     /locus_tag="PK829_10810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU41745.1"
                     /translation="MSRPTHSAGGRQVALALMVLALLVAALLRLPQLTAIPPGVHYDE
                     AAYGLNAGDIGLRGDRPIFIPSFTGREVLYLYAAGGMARLLGNTLFALRLTSVFFGLL
                     TIAATYRLGREMLADRRIALLAAALLAVSFWHVLFSRLGFRVISQPLLQALAVAALFR
                     ALRGGRWPWLWLAGVLIGLTAYTYLAARLFPLLLAAALLPLLLDRDTRPLRARQLLFV
                     ALVAFLTVLPLLAYFAIHPDAFWVRIGQVAPGAEEGLTLTESLLRALSMFFLRGDPYL
                     RFNLPGRPLFDFVTGGLLLAGWVICLRRYRRFPYDWQRAAVLLLLLAPLVMILPTALA
                     TNEIVPSNLRAMGLIPFVFFLPPIGLITLLRDVERRFGRPNLSAAVQVIFLVVLWAGG
                     QWTQHLYFRVWAADEELVFVSDGDMTAAAGFLDRTLAALPADERPAVYMASPHYRHPT
                     LAFLSANYDAIRWLPGSHALVFPAAGPALYLYPASSPAPDWAIPYLESDGPPEIV"
     gene            complement(1669..>3546)
                     /locus_tag="PK829_10815"
     CDS             complement(1669..>3546)
                     /locus_tag="PK829_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU41746.1"
                     /translation="LDSFGAPATVVEINQGPTITQFGVEPNYLVMRGGKRTKVKVGKI
                     ASLADDLALALAARSIRIQAPVPGKGYVGIEVPNSAKALVSLRDVMESDEYARMKSPL
                     RIGLGQNVAGQPIVADLAAMPHLLIAGTTGSGKSVCVNGLIACLLLQNTPDDLRLVMV
                     DPKRVELTGYNGVPHLAAPVVVDMDRVIGTLQWALREMDNRYKLFSGIGARNIVEYNK
                     KVRREPTTDGVPGSRGQVPGEERSAPAPGSGRPTAYKLPYIVIVIDELADLMMLAPED
                     TERSITRLAQMARATGIHMVIATQRPSVDVVTGLIKANFPARIAFAVASSTDSRVILD
                     TTGAERLLGQGDMLFQSPDAAAPVRLQGCFVSDGELQKLISYWQTARRTQFIAAPAGK
                     VPGARGQVTGTRGHGLGSREDRSSLEPGTSNLEPDAYPPVPPPISHLNAPEPMGGPPP
                     PQRRAPRIPTAPAGTTTDGQPPTAARGDSRRPTTDDRPQVPGVERSSPGPSANFEPYV
                     PPGAPHTQPPLWETLQAIDGDDGSGDNGEADDLLPEAIALVRSLNKASTSLLQRRFRI
                     GYTRAARLMDALEEAGIIGPPTGTSKAREVYGSARTADEGDSDAAAEEGDGAGEDTEG
                     A"
BASE COUNT          578 a         1130 c         1328 g          510 t
ORIGIN      
        1 gacgatctcc ggcgggccgt cgctctccag gtaggggatg gcccagtcgg gcgcggggct
       61 gctggccggg tagaggtaca gcgccgggcc ggcagcgggg aagaccaggg cgtggctgcc
      121 gggcagccag cggatggcgt cgtagttggc gctcaagaag gccagcgtcg ggtggcggta
      181 gtggggtgag gccatgtaga ccgccggccg ctcgtccgcg ggcagggccg ccagtgtccg
      241 gtcgaggaaa ccggcggcgg cggtcatgtc gccgtcgctg acgaagacaa gctcctcgtc
      301 ggccgcccag acgcggaaat agagatgctg cgtccactgc ccgccggccc agaggacgac
      361 caggaagatg acctggacgg cggcgctcag gtttggccgg ccaaagcgcc gctccacgtc
      421 gcgcagcagg gtgatgaggc caatgggtgg caggaagaag acgaagggga tcaggcccat
      481 cgcccgcagg ttgctgggga cgatctcgtt ggtggccaga gccgtgggca ggatcatcac
      541 cagcggagcc agcagcagca gcagcacggc ggcccgctgc cagtcgtagg ggaagcgccg
      601 gtagcggcgc aggcagatga cccagccggc cagtagtagc ccgccggtga caaaatcgaa
      661 cagcggccgg ccgggcaggt tgaagcgcag atatggatcg ccacgcagga agaacatgct
      721 cagggcacgc agcaggctct cggtcagcgt cagtccctcc tccgcgccgg gggcgacctg
      781 gccgatacgc acccagaagg cgtcggggtg gatggcgaag taggccagca gcggcagaac
      841 ggtcaggaag gcgaccaggg cgacgaagag cagttggcgg gcgcgcaggg ggcgcgtgtc
      901 gcggtcgagc agtagcggca gcagggcggc ggccagcagc agcgggaaga ggcgggcggc
      961 caggtaggtg taggcggtca ggccgatgag cacgcccgcc agccacagcc acggccagcg
     1021 gccgccacgc agcgcccgga acagcgccgc cacggccagc gcctgcaaca gcggctggct
     1081 gatgacgcgg aagcccagac ggctgaagag tacgtgccag aagctgaccg ccagcagggc
     1141 ggcggccagc agagcgatgc gccggtcggc cagcatctcc cggccaagcc ggtaggtcgc
     1201 ggcgatggtc agcaagccga agaagacgga ggtcaggcgc agggcgaaga gggtgttgcc
     1261 cagcaggcgg gccatgcccc cggccgcgta aaggtagagc acctcccggc cggtgaagct
     1321 ggggatgaag ataggccggt cgccgcgcag gccgatgtcg ccggcgttga ggccataggc
     1381 ggcctcgtca taatggacgc cgggcggaat cgcagtcaac tgcggcagcc gcagcagcgc
     1441 cgccaccagc agggccagaa ccatgagggc cagggcaacc tgccggccgc cggcagagtg
     1501 ggtcgggcgg gacatgaggc ggattgtagc aggtgcgggg cggagcgaca ggggacggtg
     1561 gacggcggcg cgtccgcctg tcaggtcatt gggtaactgg cgaacactga aagcccaccc
     1621 cgcccaccgc ccctatgaag aggccaggcg atgtcaccgc cgacagcctt acgctccctc
     1681 agtgtcttcg ccggccccat ccccctcttc tgccgcggca tcgctatccc cctcatccgc
     1741 cgtccgcgcg gaaccgtaga cttcccgcgc cttgctcgtg cccgtcggcg ggccgatgat
     1801 gccggcctct tccagcgcgt ccatcagccg ggcggcgcgg gtgtagccga tgcggaagcg
     1861 gcgctgcaac agcgacgtgg acgccttgtt gagcgaccgc actagggcga tggcctctgg
     1921 caataggtcg tcggcctcgc cgttgtcgcc gctgccgtcg tcgccgtcta tggcttgcag
     1981 cgtctcccac agcggcggct gggtgtgcgg cgcgcctggc ggcacgtatg gctcgaagtt
     2041 ggcggagggg ccaggggaag aacgttctac cccgggaacc tgcggcctgt cgtctgtcgt
     2101 cgggcgacgg gagtcgcctc tggcggcagt tgggggctgg ccgtcggtgg tcgttcccgc
     2161 cggcgcggtg ggaatgcgcg gggcgcgacg ttgcggcggc ggtggcccgc ccatcggctc
     2221 cggcgcgttc aggtgggaga tgggcggggg gacgggcgga taggcgtcag gttccaggtt
     2281 cgaggttcca ggttccaggg aggagcggtc ttccctagaa cctagcccgt gacccctagt
     2341 ccctgtaacc tggcccctgg cccctggaac cttccccgcg ggcgcggcaa tgaactgcgt
     2401 ccgccgcgcc gtctgccaat agctgatcag cttctgcaat tcgccgtcgc tgacaaagca
     2461 gccctgcaag cgcaccggcg cggccgcgtc cggactctgg aagagcatgt cgccctgacc
     2521 cagcagccgc tccgcgccgg tcgtgtccag aatgacgcgg ctgtcggtgc tgctggccac
     2581 ggcgaaggca atgcgcgccg ggaagttggc tttgatcagt ccggtgacaa catcgaccga
     2641 cggccgctgc gtggcgatga ccatatggat gcccgtcgcc cgggccatct gcgccagccg
     2701 ggtgatgctg cgctcggtgt cctccggggc cagcatcatc aggtcagcca gctcgtcgat
     2761 gacgatgacg atgtagggga gtttgtaggc cgtggggcga ccgctgccgg gggccggggc
     2821 agagcgttct tcccctggaa cctggcccct ggaacctggg actccgtcgg tcgtcggttc
     2881 gcggcgaacc ttcttgttat actcgacgat gttgcgcgcc ccgatcccgg agaagagctt
     2941 gtagcggttg tccatctccc gcaaagccca ttgcaacgtg ccgatgaccc ggtccatgtc
     3001 cacaacgacg ggcgcggcca ggtggggcac gccgttgtag ccggtcagtt cgacccgctt
     3061 gggatcgacc atgaccaggc gcaggtcgtc gggcgtgttc tgcaacagca ggcaggcgat
     3121 cagcccgttg acacagaccg atttgccgga gccggtcgtg ccggcgatga gcagatgggg
     3181 catggccgct aggtcagcga cgatgggctg cccggcgacg ttctgcccca ggccgatgcg
     3241 cagcggcgac ttcatgcggg cgtactcgtc cgactccatc acgtcgcgca gggagacgag
     3301 ggctttggcg ctgttgggca cttcgatgcc cacgtagccc ttgccgggca caggggcctg
     3361 gatgcggatg gagcgggccg ccagggccag ggccaggtcg tcggccaggc tggcgatctt
     3421 gccgaccttg accttcgtcc gcttgccgcc gcgcatgacg agatagttgg gttcgacgcc
     3481 gaactgggtg atggtcggcc cctggttgat ctccacgacg gtggccggcg cgccgaagct
     3541 gtcgag
//
LOCUS       DAOSMQ010000337         3537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26557_length_3537_cov_4.506605, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000337 DAOSMQ010000000
VERSION     DAOSMQ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26557_length_3537_cov_4.506605"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /gene="rnpB"
                     /locus_tag="PK829_10820"
     ncRNA           complement(<1..379)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK829_10820"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            495..1100
                     /locus_tag="PK829_10825"
     CDS             495..1100
                     /locus_tag="PK829_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41747.1"
                     /translation="MTRTQPPAAGRVLRLAAALLAVAAVALGLLIALGAFDPRPLGPL
                     FRSTYPGSLRLAGEGEFFQWRPSPFAALPARRSLRLTAAHVSGEMDSGYGLTLGDGAL
                     TVAVSPLGEAAVWTAMEAEKRYLLPWQPWPHVQTGAAGNEIWLDIDATTDPARVTAWI
                     NRERLWQGEIAPPSGEVAIWLGSAGGAVTVDFTALTWYGDG"
     gene            complement(1097..>3537)
                     /gene="polA"
                     /locus_tag="PK829_10830"
     CDS             complement(1097..>3537)
                     /gene="polA"
                     /locus_tag="PK829_10830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU41748.1"
                     /translation="QMAGRVAVHIITGDRDLLQLVDDNTRVELPSRKPGGSPETYDAA
                     GVVAKLGVRPEQVVDYKALVGDVSDNIPGVKGIGEKTAVKLLEEYGTLANLYAHIDDI
                     KGALRQKLVDGEHSARLSYDLARIVTDAPVTVALEDCHTRDFNAAEAISLFRALEFRS
                     LTNALVAHVGAPDDLPQVVAAGEAARKTEVVLVRDATALAEMVARLERAEWISFDVET
                     DSLERMLAGIVGICLAVEPPVAYYIPIGHVVGASQAGSGQMNLFAGEAAPAPGQLPLA
                     QVLDAIRPAMTNPAIRKVAHNAKFDCMILSRHGLAVSPVAFDTMIAEWLTDPATKHKG
                     LKDLARHRLGAEMTDIETLIGRGKTQVTFAQVPIETAAPYGAADADMTLRLMPLLQQE
                     IEAKGLTRLLDLEMELLPVLADMERIGVRIDVDFFRQMSQELAVVLLRLEGTIYEMAG
                     EPFNINSTQQLSDILFGKLRLPREGLKKIASGHYSTAADVLDGLRAVDKSGIVDAIIE
                     HRELTKLKGTYVDALPLLINPNTGRLHTSFSQIGAVTGRLASSNPNLQNIPVRSEVGQ
                     KMRRGFISEPGWVFLSADYSQVELRILAHISQDETLLESFRNDRDIHTTTAAAVYSIP
                     PEKVTRNQRNFAKRVNFGLIYGMSSFRLARESGLTPGEAETFVKRYFAQFPGVERYLE
                     ATRIMARTDGYVETLFGRRRYFPVFKSPMSSANRQAVMRAEREAVNHPIQGTAADIIK
                     IAMVRLHAQLSGRYQARMLLQVHDELLLELPVEEVEAVRPMVIEIMCDAYELDAPLRV
                     EAETGANWLELKSG"
BASE COUNT          529 a         1179 c         1178 g          651 t
ORIGIN      
        1 agccgcattc tgtcccggcg ggcggcgaac cgtccgccgg cggtgatcat ctatctggga
       61 tcgccattac tggcgacctc gtgcggccta cccggacgtc agacggaacg agccgctccg
      121 taggcgacca gcgcctttcg ccctgcttgg ccttgctcct ggtggggttt gcctggccgg
      181 cgacatcgct gccgccgccg gtggtctctt acaccaccgt ttcaccctca ccacaagggt
      241 tgccccctgt ggcaatacgc ctctctgttg cacttgccgt cgagtcgccc cgcccggctg
      301 ttagccggcg ccctgctctg tggagtgcgg actttcctca gccccggcga accggagccg
      361 cgatcaccca gcctgcttgc taaccgtgat cctaaccatc tttcggtcgc ttggcaacct
      421 gtcttctcgg gaatgcgccg catggagagc caccgccctt gcgccccgcg cccaaggggc
      481 ctataaatcg attgatgacc cgtacccagc ccccggccgc cgggcgcgtc ctgcgcctgg
      541 ccgcggcgct tctggccgtc gccgctgtgg cgctcggcct gctaatcgct ctgggcgcgt
      601 tcgacccccg gccgctcggc ccactgttca ggagcaccta ccccgggtcg ctgcggctgg
      661 cgggcgaggg tgagttcttc cagtggcggc cgtcgccctt cgccgctctg ccggcgcgcc
      721 gttcgctccg tctgacagcc gcccacgtca gcggggagat ggacagcggc tatggcctga
      781 cgctggggga tggcgcgctc actgtggccg tgtcaccgct gggggaggcc gccgtgtgga
      841 cggcgatgga ggcggagaaa cgctatctgc tgccgtggca gccctggccc cacgtgcaaa
      901 cgggcgcggc cggcaacgaa atctggctgg acatcgacgc cacgaccgat cccgcccgcg
      961 tgactgcctg gattaaccgc gagcgcctgt ggcagggcga gattgcgcca ccatcgggcg
     1021 aggtcgctat atggctgggc agcgccggcg gcgcggtgac ggttgatttc accgcgctga
     1081 cgtggtatgg cgacggctaa ccgctcttca actccagcca attcgcgccc gtctcagcct
     1141 ccacgcgcaa cggcgcatcc agctcgtagg cgtcacacat gatctcgatg accatcggcc
     1201 ggacagcctc gacttcttcg accggcagct ccagcagcag ctcatcgtgc acttgcagca
     1261 gcatccgcgc ctggtaacgg ccgctcagct gcgcgtgcag ccgcaccatg gcgatcttga
     1321 tgatgtccgc ggccgtgccc tggatggggt ggttgacagc ctcgcgctcg gcgcgcatga
     1381 ccgcctggcg gttggcgctg ctcatgggcg acttgaagac cgggaagtag cggcggcggc
     1441 cgaagagcgt ctccacgtag ccgtcggtgc gggccatgat gcgcgtcgct tccaaatagc
     1501 gctccacgcc ggggaactgg gcaaagtacc ttttgacgaa cgtctccgcc tcgcccggcg
     1561 tcaggccgct ctcgcgcgcc agacggaagg aactcatgcc atagatgagg ccgaagttga
     1621 cccgcttggc gaagttgcgc tggttgcgcg tcaccttctc cggcggaata ctgtagacgg
     1681 cggcggccgt cgtcgtgtgg atgtcgcggt cgttgcggaa ggattccagc aacgtctcgt
     1741 cctggctgat gtgggccagg atgcgtaact caacctgcga gtaatcggcc gagagaaaca
     1801 cccagcccgg ttcgctgatg aagccgcgac gcattttctg gccaacttcg ctgcggacgg
     1861 ggatgttttg caagttgggg ttgctgctgg ccagccggcc agtcaccgcg ccgatctggc
     1921 tgaagctagt gtgcaggcgg ccggtgttgg gattgatcag cagcggcaaa gcatcgacgt
     1981 aggtgccctt cagtttggtc agttcgcggt gttcgatgat agcgtcaacg ataccgctct
     2041 tgtccaccgc tcgcaggccg tcgagcacgt cggcggcggt cgagtagtgg ccgctggcga
     2101 tctttttcag gccctcgcgc ggcaggcgca gcttgccaaa gaggatgtcg ctgagttgct
     2161 gggtcgagtt gatgttgaat ggctcgccgg ccatctcgta gatagtgcct tccagccgca
     2221 gcagaaccac ggccagttcc tgcgacatct ggcggaagaa atcgacgtcg atgcgcacgc
     2281 cgatccgctc catgtcggcc agaaccggca atagctccat ctccaggtcg agcagccgcg
     2341 tcaacccctt ggcctcaatc tcctgctgca agaggggcat caggcgcaac gtcatgtcgg
     2401 cgtcggcggc tccatagggc gcggccgttt cgatgggcac ctgggcgaac gtcacctggg
     2461 ttttgccgcg gccgatgagc gtctcgatat cggtcatctc cgcgcccagc cggtggcggg
     2521 ccaggtcctt cagtcccttg tgcttcgtcg ccgggtcggt cagccactcg gcgatcatcg
     2581 tgtcgaaggc taccgggctg accgccaggc cgtggcggct gaggatcatg cagtcgaact
     2641 tggcgttgtg ggccaccttg cggatggccg ggttggtcat cgccggtcga atcgcgtcga
     2701 gcacctgcgc cagaggcagc tggcccggcg cgggcgcggc ttcgccagca aagaggttca
     2761 tctggccgct gccggcctgc gacgcgccaa caacgtggcc aatagggatg taataggcca
     2821 ccggcggctc gacggccaga cagatgccga cgatgccggc cagcatccgc tccaggctat
     2881 cggtctccac gtcgaagctg atccactccg cccgttccag ccgggccacc atctcggcca
     2941 gcgcggtcgc gtcgcggaca aggacgacct cggtcttacg ggccgcttcc ccggcggcga
     3001 cgacctgcgg caggtcgtct ggcgcgccta cgtgggccac cagagcgttg gtcagcgagc
     3061 ggaactccag cgcgcgaaag aggctgatgg cctcggcggc gttgaagtcg cgggtgtggc
     3121 aatcctccag cgcgacggtg acgggcgcgt cggtgacaat gcgggccagg tcatagctca
     3181 gccgggccga gtgctcgccg tcaaccagct tctggcgcaa ggcgcccttg atgtcgtcga
     3241 tgtgggcgta gagattggcc agcgtgccat attcctccag cagcttgacg gccgtcttct
     3301 cgccaatgcc cttgacgccg gggatgttgt cgctgacgtc gccgacgagg gccttgtagt
     3361 ccacgacctg ttccggccgc acgcccagct tggccaccac gccggcggcg tcgtaggtct
     3421 ccgggctgcc gccgggcttg cgcgatggca actcgacgcg ggtattgtcg tctaccagtt
     3481 gtagcaggtc gcggtcgccg gtgatgatgt ggacggcgac gcggccggcc atctgcc
//
LOCUS       DAOSMQ010000338         3536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26568_length_3536_cov_5.173801, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000338 DAOSMQ010000000
VERSION     DAOSMQ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26568_length_3536_cov_5.173801"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK829_10835"
     CDS             <1..234
                     /locus_tag="PK829_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOU41749.1"
                     /translation="RTLTLDHVVPRRYGGPHTWTNLVAACSGCNRRKGGKLPAEADMI
                     LRRLPFEPSPTAAYRFGTHLEQRQEWAQFIEGW"
     gene            complement(247..720)
                     /locus_tag="PK829_10840"
     CDS             complement(247..720)
                     /locus_tag="PK829_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOU41750.1"
                     /translation="MARILVICTANICRSPVAAALLRDRLRQRGLGDWTVGSAGTWAI
                     AARPASRYSVDVMGRHGFNIADHRATMVEERHLRDADLALTMEDGHAEALRAEFPAHA
                     HKVYLLTEMIGRGYNIPDPYGGPVAGYQQMYDELVAVIDAGMERVITLARQNARA"
     gene            complement(734..1291)
                     /gene="raiA"
                     /locus_tag="PK829_10845"
     CDS             complement(734..1291)
                     /gene="raiA"
                     /locus_tag="PK829_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOU41751.1"
                     /translation="MELQILSNNMDITPRLRDYVEKKTARLDRYLPKLSAIQVVLAME
                     NTRSAVQRHVAEITIRDERGTILRAEERNSDMFAAIDAVIDKLYRQIERYRGKNRAKT
                     RGKAEDIDMGEPLPIAEELHEEDHAIVRYKRFALHPMSAEEAIDQMELLGHDFYVFFN
                     AEDNAVNVLYRRRDGTFGLLQPEMG"
     gene            complement(1386..1868)
                     /locus_tag="PK829_10850"
     CDS             complement(1386..1868)
                     /locus_tag="PK829_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOU41752.1"
                     /translation="MRAPLRYAEPTSSLIHRLKYEGCFALGGALAETLIAGWPRWLRP
                     PDLIVPIPLHPRRRRQRGYNQSELLARPLAEAVGVAYSATPLQRTRHTQPQVGLGPQA
                     RAVNVRGAFTAAPDEARGRAILLVDDVLTTGATMTAAAEALLAAGAASVSAYCLARVS
                     "
     gene            complement(2129..2428)
                     /locus_tag="PK829_10855"
     CDS             complement(2129..2428)
                     /locus_tag="PK829_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41753.1"
                     /translation="MTDRTTNDDKKPEWELSEIRVRRDSPTVGARDAFLELKDQLCRR
                     ILALRDPALDLSNQPQIRPFVHDRLDALLDERQIVLNRSEKRQLLEAIVAELSQL"
     gene            complement(2466..2630)
                     /locus_tag="PK829_10860"
     CDS             complement(2466..2630)
                     /locus_tag="PK829_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41754.1"
                     /translation="MRFLRGDDGQGLVEYALVLVLVAVVLIAILALLGPYISQWLSNA
                     GEWFRQALSS"
     gene            complement(2667..3407)
                     /locus_tag="PK829_10865"
     CDS             complement(2667..3407)
                     /locus_tag="PK829_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU41755.1"
                     /translation="MEPIKLLIVDDHPLFRQGLVDVLETDPSMSVVGEAADGETAVER
                     AAEFVPDIILMDVNLPKMNGLQVTRRVLSQNPALKVIILTGYDDAEQVYHALRLGAVA
                     YCAKDVPPDELLDTIHAVQKGAYVINNQVMTDDEIQAWFQRRATRYGTYPTEPAEPVE
                     GQPAGAPASTVAITPLSPREMEILELVTRGASNKDIANRLTISEQTVKNHMTAVLRKL
                     RVEDRTQAAVYALRHGWVRLEAARPPSQ"
BASE COUNT          618 a         1138 c         1153 g          627 t
ORIGIN      
        1 cgcacgctga cgctcgatca cgtcgtgccg cggcgctatg gcggcccgca cacctggaca
       61 aacctcgtcg ccgcctgctc tggctgtaac cgccgcaagg gcggcaagct gccggccgaa
      121 gccgacatga tcctgcggcg gctgccgttt gaacccagcc ccacggcggc ctatcgcttc
      181 ggcacgcacc tggagcagcg ccaggagtgg gcgcagttca tcgaaggttg gtaagctgaa
      241 gcgcggctac gcgcgcgcat tctgccgggc cagggtgatg acgcgctcca tgccggcgtc
      301 gatcaccgcc accaactcgt cgtacatctg ctgatagccc gccaccgggc cgccgtaggg
      361 gtcggggatg ttgtagccgc ggccgatcat ctcagtcagc aaatagacct tatgggcgtg
      421 cgcggggaac tcggcccgca gcgcttcggc gtggccgtcc tccatcgtca gggccaggtc
      481 agcgtcgcgc agatggcgct cttcgaccat cgtcgcccgg tggtcggcga tgttgaagcc
      541 gtggcggccc atgacatcga cgctatagcg cgaggccggc cgagcggcta tggcccacgt
      601 cccggccgag ccgaccgtcc agtcgcccag cccgcgctgg cgcagccggt cgcgcaacag
      661 cgccgcggcc accggcgacc ggcagatgtt agccgtgcag ataacgagaa tgcgcgccat
      721 gctgcctgcc ggcttagccc atttccggct gtagcaggcc gaaagtgccg tcgcgacgac
      781 ggtagaggac gttgacagcg ttgtcctcag cgttgaagaa aacatagaag tcgtggccca
      841 gtaactccat ctggtcgatg gcctcctcgg ccgacatggg gtgcagggca aagcgcttgt
      901 agcgaacgat ggcgtggtct tcctcgtgca gttcctcggc aatgggcagc ggctcgccca
      961 tatcgatgtc ttcggccttg ccgcgcgtct tggcgcggtt cttgccgcgg tagcgctcga
     1021 tctggcggta gagcttgtcg atgacagcat cgatggcggc gaacatgtcg ctgttgcgct
     1081 cctcagcgcg caggatggtg ccgcgttcgt cgcggatggt gatctcggcc acgtggcgct
     1141 ggacggcact gcgcgtattc tccatggcca aaaccacctg gatagcagac agcttgggga
     1201 gatagcgatc gaggcgcgcc gtcttctttt ccacgtagtc gcgcaggcgt ggggtgatgt
     1261 ccatattgtt cgagaggatt tgcagttcca tcttgacctc tactccttat aacaagaatt
     1321 ggtgcgtcgt tggcaggccc gccggccgga aggtatcgtg ttgggaatac ggcattatcc
     1381 catgactagg acacacgcgc caaacaatag gccgagacag aagcggctcc ggcggccaac
     1441 agtgcctcgg ccgcggcggt catggtcgcg ccggtggtca gcacgtcgtc aacgaggagg
     1501 atagcccggc cccgcgcttc gtccggcgcg gccgtaaacg cgccccgcac attgacggcg
     1561 cgcgcctgcg gccccagccc aacctggggc tgggtgtggc gcgtgcgctg gaggggcgtg
     1621 gcgctatagg ccacgcccac cgcctcggcc agcggccggg ccaacagttc cgactgatta
     1681 tagccgcgct gccgtcgccg gcgcgggtgc agggggatgg gcacgatgag gtcaggcggc
     1741 cgcaaccaac gcggccaccc ggcgatgagc gtttcggcca acgccccgcc cagggcgaag
     1801 cagccctcgt acttcaggcg gtggatgagg ctgctggtcg gctcggcata gcgcagcggg
     1861 gcgcgcatct gagtcagcgg ggacggagcg gccgcacagc cgtggcaaag gtcagctgtc
     1921 cggccctcct cgccggccag ggatcgaccg cagcaaaaac agatcggccc caccacgggc
     1981 gcaagacgcg ccgcgcaggc cgggcagagc aacacgccga cgcgcccaca gccggcgcag
     2041 accgacggaa agagtacgtc caggcagaat gcccccacgc gccgccacgt agcgccggag
     2101 aaggccggtg aaacggaagt gctcacgcct acaactggga aagctcggcg acgatggcct
     2161 cgagtagttg tcgtttctcg ctgcgattca ggacgatctg gcgctcatcc agcagagcat
     2221 ccaatcggtc gtggacaaac ggccgaatct ggggctggtt gctcaggtca agcgccgggt
     2281 cgcgcagggc cagaatgcgg cggcagagtt ggtctttcaa ctccagaaag gcgtcgcgcg
     2341 cgcccacagt cggcgagtcg cggcgcacac ggatttcgct tagttcccat tccggtttct
     2401 tgtcgtcgtt tgttgtccgg tctgtcacga ttgctcccac gatccacacc ggcagtcgag
     2461 cgccattagc tgctcagcgc ctgtcggaac cattcaccgg cgttgctgag ccactggctg
     2521 atataggggc cgagcaaggc caaaatcgcg atgagcacga cagcgaccag taccaatacg
     2581 agggcatatt ccaccagccc ctgaccatca tcgcctctca agaagcgcat atgttcgttc
     2641 ctccgcagcc aactgacggc tggccgctat tggcttggcg gcctggctgc ctccaggcgc
     2701 acccagccgt gtcgcaaggc atagacagcg gcctgcgtgc gatcctcgac gcgtagcttg
     2761 cgcaggacag cggtcatgtg gttcttaacc gtctgctcgc tgatcgtcag ccgattggcg
     2821 atgtccttgt tgctggctcc gcgtgtcacc agttccagga tttccatctc gcgcggcgag
     2881 aggggggtga tcgccaccgt cgacgcaggc gcgccggcgg gttggccttc gaccggttcg
     2941 gccggctcgg tcgggtatgt gccgtagcgc gtggctcgcc gctggaacca ggcctgaatc
     3001 tcgtcgtcgg tcatgacctg attgttgatg acgtaggccc ccttctggac ggcgtggatg
     3061 gtgtccaaca gttcgtctgg gggtacatcc ttggcgcaat aggccaccgc gcccagccgc
     3121 agggcgtggt agacttgctc ggcgtcgtcg taaccggtca ggatgatcac cttcagggcc
     3181 ggattctggc tgaggacgcg gcgcgtcacc tgtaagccgt tcatcttggg caggttcaca
     3241 tccatcagga taatgtcggg cacaaattca gcggcgcgct cgacggccgt ttcgccgtcg
     3301 gcggcctccc ccacaactga catactgggg tctgtctcca gaacatcaac cagaccctgc
     3361 cggaagaggg gatgatcatc aacgatgagt agcttgatcg gctccattgt tgtctcccaa
     3421 gctccgtagt tttacgcagt atagcacgaa gtcgcccaac attctaccgc ggtcacaatg
     3481 gacgttgccc cacacaggtg atcagatcgc cgaagttcac cggcacagcg gccgcc
//
LOCUS       DAOSMQ010000339         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26758_length_3516_cov_4.216123, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000339 DAOSMQ010000000
VERSION     DAOSMQ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26758_length_3516_cov_4.216123"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PK829_10870"
     CDS             <1..508
                     /locus_tag="PK829_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C2)-methyltransferase"
                     /protein_id="HOU41756.1"
                     /translation="TVMDHRGLAIGPRFITLSTVGVVPGIRRLTAERRPMRLAVSLHG
                     ATDEERARLVPPARRWPLTELMAACRDYTTTLGRRIFFEWTLIDGQNDGPTQAHALGR
                     LLAGMDAHVNLIPLNPTLGYDGRPTAGPAARAFQAVLAGYGLPSTVRQRRGIDVAAGC
                     GQLAVERS"
     gene            complement(622..1323)
                     /locus_tag="PK829_10875"
     CDS             complement(622..1323)
                     /locus_tag="PK829_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOU41757.1"
                     /translation="MSQVSAVAPAPQAKAATVGRVAVIVIAAYMGAQMLSDIASLKIG
                     VVAGLAVDMGTFIYPITFTLRDVVHKVTGKRSAQTLIITAGVVNLFMAAYLSWAARVP
                     SDPTWGRGEQFSAILGPVWRIVLASIIAELVSELLDTEVYHWFVTRVTTRYQWARVLT
                     SNAVSIPIDTLIFTTIAFGPLPGLANDPLSLPWATVWQIFLFNLAVKFAMTLVSLPLI
                     YTTRDRDWSADEHDA"
     gene            complement(1366..2025)
                     /locus_tag="PK829_10880"
     CDS             complement(1366..2025)
                     /locus_tag="PK829_10880"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOU41758.1"
                     /translation="MAHTGDFVLVSANGVLAGDGWLTPYLARAAAVIAADGGLRHLLA
                     LGRRPDVLIGDLDSLPPGVDPQTAADQVIRYPRDKDETDLELALLYAIERYPGAEVLV
                     AGGFGGRLDHMLANVLLLAHPRLLGQPVRFVDDRQTAWLVSAAAPGESVISGAPGDIV
                     SLLPLGGPARVVATSGLRWPLRDEVLAFGPARGVSNVMTAESATVRLDQGAVLCVHIR
                     Q"
     gene            2267..>3516
                     /locus_tag="PK829_10885"
     CDS             2267..>3516
                     /locus_tag="PK829_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOU41759.1"
                     /translation="MWRAQQLLADNLSTLGADWRNMMRRLRQAHVDYVVLPVGGPLPE
                     RAGPRRGFIERRLPLPADPLSLQHLNHRLRMVADAPNARGVVFIFRGFSAGPATLQNF
                     RAAVARLRAAGKDTIVYTPTLDLAHYYAATAAARIVAPPGAQFDVLGLYTEVTFLKDA
                     LARAGVQADVVQISPYKTAFDRYSRAEMTPEHRDQLNWLLDDQYDTLTADMAAGRGMS
                     QATLRELIDRAPLSAEAAREAGLIDWVAYDDELASLLGRDRANRAQPDTTTPTTAAGD
                     MAAAPKASLQSWARARDALLEKPRRHTRRWVGVLALEGLITMGPSRQPPIELPIPFIG
                     GAAAGEQTLVSILRQLEKIDDLAALVLHVDSGGGSVLATPLLLYVVGVRDPHVAIGTA
                     AASVALNALAGLLGQAKAGRVKWP"
BASE COUNT          599 a         1202 c         1161 g          554 t
ORIGIN      
        1 aacggtgatg gatcatcgcg gcctggccat cggcccacgc ttcatcaccc tcagcaccgt
       61 cggcgtcgtg ccgggcatcc gccggctgac ggccgagcgc cggcccatgc gcctggccgt
      121 cagcctgcac ggggccacgg acgaggagcg cgcccggctg gtgccgcctg cccgccgctg
      181 gccgctgacc gagttaatgg ccgcttgccg cgactacacc accacgctgg gccggcgcat
      241 cttcttcgag tggacgctca ttgacggcca gaacgacggc ccgacccagg cccacgccct
      301 cggccggctg ctggcgggca tggacgccca cgtcaacctc attcccctga accccacgtt
      361 gggctacgac ggccggccca cggccgggcc ggcggcgcgc gccttccagg ctgtcctggc
      421 cggctacggc ctacccagca ccgtccgcca gcgccgcggc atcgacgtcg ccgccggctg
      481 cggccaactc gctgtcgaaa gaagttaacg caaagaagtt aacgcaaggg cgcaaagaca
      541 cgaagacgca aggacgcaaa agcataaatc cttttgcgtc cttgtcctaa cctcttctcc
      601 caccgctccc ccgctgactt cttatgcgtc gtgctcgtcg gccgaccaat cgcggtcgcg
      661 cgtggtatag atgagcggca ggctgacgag ggtcatggcg aacttgacgg ccagattaaa
      721 taggaagatt tgccacaccg tcgcccacgg taggctaagc ggatcgttgg ccagccccgg
      781 cagcgggcca aaggcaatgg tggtgaagat gagcgtgtcg atggggatgc tgacggcgtt
      841 gctggtgagc acgcgcgccc actggtagcg cgtcgtcacg cgggtgacga accagtgata
      901 gacctcagtg tcgagcaact cgctaacaag ctcggcaatg atcgacgcca ggacgatgcg
      961 ccacaccggg ccgaggatgg cgctgaattg ctcgccccgg ccccaagttg ggtcgctggg
     1021 cacgcgcgct gcccaggaca ggtaggcggc catgaacaga ttgacgacgc cggcggtgat
     1081 gatcagcgtt tgggcgctgc gtttgcccgt gaccttgtga accacgtcgc gcagggtgaa
     1141 ggtgatggga tagatgaacg tgcccatgtc cacggccagc ccggcaacga cgccgatctt
     1201 gaggctggcg atgtcggaca acatttgcgc gcccatgtag gcggcgatga cgatcacggc
     1261 cacgcggccg acggtggccg ctttggcctg tggagccggg gctactgcac tgacttgact
     1321 catctgacga tctccttgtt ttggtaaggc gggagctgcg accgcctact gcctgatgtg
     1381 gacgcacaag accgcgccct ggtcgagccg aaccgtggcc gattcggcgg tcatcacgtt
     1441 gctgacgccg cgcgccgggc caaaggccag cacctcgtcg cgcaggggcc agcgcagtcc
     1501 gctggtggcc accacacggg ccggcccgcc cagcggcaat agcgagacga tgtcgcccgg
     1561 cgcgccgcta ataactgatt cgcccggcgc agccgcgctg acgagccagg ccgtctgccg
     1621 gtcatcgacg aaacgcaccg gctggcccag caggcgcggg tgggccagca acaggacgtt
     1681 ggccagcatg tggtcgagcc ggccgccaaa cccgccggcg acgagcacct ctgcgccggg
     1741 gtagcgctcg atggcgtaga gcagggccaa ctccaggtcg gtttcatcct tgtcgcgcgg
     1801 gtagcggatg acctggtcgg cagccgtctg cgggtcaacg ccgggcggca gcgaatcgag
     1861 gtcgccgatg agcacgtccg ggcggcggcc gagggccagc aggtggcgca ggccaccatc
     1921 ggcggcgatc acagctgcgg cccgcgccag ataaggcgtc agccagccat cgccggccag
     1981 cacgccattg gcagaaacga gaacaaaatc gcccgtatgc gccatcgagt tcactccgcg
     2041 agacaaagtg agggcgaatg gtagcccgtg ccacagggcc gcgccacagg aatcgcgctt
     2101 gtttgaccaa agcgtccctc tgagattaca ctttgggtat ctgcccaagt tcctaacaag
     2161 agaagtgact aagcctgtgg ctgtaaagtt tcgcccatcg tcccttgtca tagaccctgt
     2221 gccggcgaag acatcacccg gtttttccaa agcgctccgc cgcgacgtgt ggcgggcgca
     2281 acagttgttg gccgacaatc tgagcacact gggggccgat tggcgtaaca tgatgcggcg
     2341 gctgcgccag gcccacgtcg attacgtcgt cctgcccgtc ggcgggccgc tgccggagcg
     2401 ggccgggccg cggcgcggct tcatcgagcg ccgcctgccc ttgccggcag atccgctcag
     2461 cctccagcat ctaaaccacc gcctgcgcat ggttgccgac gctcccaatg cccgcggcgt
     2521 agtgttcatc ttccgcggct tcagcgccgg cccggccacc ttgcagaact tccgcgccgc
     2581 tgtggcccgg ctgcgcgccg caggcaagga cacgatcgtc tacacgccaa ccctcgacct
     2641 ggcccactac tatgccgcca ccgcggccgc tcgcatcgtc gccccaccgg gggcgcaatt
     2701 cgacgtgctt ggcctctata cggaggtgac cttcctcaag gacgccctgg cccgtgccgg
     2761 cgtacaggcc gacgtcgttc agatttcgcc ctataagacc gctttcgacc gctatagccg
     2821 ggcggagatg acgccagagc accgcgacca gttgaactgg ctgctcgatg accagtacga
     2881 cacgctgacg gccgatatgg ccgccggccg gggcatgagc caggcgacct tgcgtgaact
     2941 catcgaccgt gcgccgttgt cggccgaggc ggcgcgcgaa gctgggctga ttgattgggt
     3001 ggcctacgat gatgagttag ccagcctgct gggccgcgac cgggccaacc gcgcgcagcc
     3061 cgacacgacg acgccaacga cggcggctgg cgacatggcc gccgcgccta aagcctcgct
     3121 ccagtcatgg gccagggcgc gcgacgcgct gctggagaag ccccgtcgcc acacgcgccg
     3181 ttgggttggc gtcctggcgc tggaagggtt gatcaccatg gggccgtcgc gccaaccgcc
     3241 catcgagttg cccatccctt tcatcggcgg cgcggccgcg ggcgagcaaa cgctggtatc
     3301 cattttgcgc caactggaga agatcgacga cctggccgcc ctggtgctcc atgtcgattc
     3361 cggcggcggc tcggtgctgg ccaccccgct gctgctctat gtggtggggg tgcgcgatcc
     3421 ccatgtggcg atcggcaccg ccgccgcctc ggtggctctg aacgccctgg ccggcctgct
     3481 gggccaggcc aaggccggac gggtgaagtg gccctg
//
LOCUS       DAOSMQ010000340         3497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26952_length_3497_cov_4.402673, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000340 DAOSMQ010000000
VERSION     DAOSMQ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26952_length_3497_cov_4.402673"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="PK829_10890"
     CDS             <1..1351
                     /locus_tag="PK829_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41760.1"
                     /translation="TRLYASGRIWYSPAVMEVTAPQSSMPSGAATDVRLSRLAWAVTA
                     AGLALLGGAVVLILLRATRAPQAGDVPWYNDLIVTASLAVAIVVGGFVAARLPRNPYG
                     WLLLAFGIGNGSIQSFAVDYILVSHQITPLPLTSWAFYFAAFGFSLWLSAIPLLFLLF
                     PSGRLPSRRWRWLAAVVVLSFLTLAALMWLSPSAILVSVPSPFHHDNALGRWADNLTS
                     TAVFFILLAVLISAISIVVRAIRARGLERQQFKWLGLASVVMVLAFFFNTELVPLLPG
                     VFDALLEAAAFAGVPLAVGIAVLRYRLWDIDVIIRKTAQYSVLSTLLAVVFYVLVIVL
                     QEAFGRVTESDSPAAVLLSTLAIAALFAPLRRAVQNVIDRRFYRRKVDAEKVLADFAA
                     TVRDETDLDRLTAELARVIQEAMQPEHVSVWLREPAGGRRATAEGRAVATSDETRVA"
     gene            complement(1348..3342)
                     /locus_tag="PK829_10895"
     CDS             complement(1348..3342)
                     /locus_tag="PK829_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOU41761.1"
                     /translation="MSICHFDTLLIANRGEIARRIIRACRRLGIRAVAVYSDADVDSP
                     HVAEADLAVRLGPAAAAESYLNIPALLDAAARAGADAVHPGYGFLAENAAFARACVAA
                     GLVFVGPPAEAIAAMGDKRAARRLAAGLGVPVLAGYDGEADDDATLASEAARVGYPLM
                     VKAAAGGGGKGLRLVARPDDLPAALAGARREATAAFGDGRLLLERAVLRPRHVEVQVL
                     ADSHGHTLHLGERDCSVQRRHQKVIEESPAPGLSDGLRAEMGAAAVRLAAAIGYTGAG
                     TVEFLLDEAGRFAFLEMNTRLQVEHPVTEMVTGLDLVEWQLRVAAGEPLPWSQAGIAP
                     TGHAVEARLYAENPAADFLPDTGTVLLWQPAASEGVRVDDGIRTGSVVTSHYDPLLAK
                     LIATGPTRADALRRLERALAHTVLLGLTTNLAYLRAIVTRPAFASPAALSTHFLEDEL
                     PDWRPAVSAEASAVALIAVTVARWRRLSAAGPGYWRNNPGEPAPFRYRLGGETVDVRL
                     RPTPWTTGQYHVTLPGDASPRLVTLEEDAAPNLTLTIDGARRHVALANAGDTWWAQTD
                     DGPLRLTALPLWPEPHRAVDAAGSLRAPLPGTVLAVLVAVGQRVEEGQALVKIEAMKM
                     EHTIRTAAAGIVEAIHYAPGDSVDADALLAHIRPPDEAPG"
BASE COUNT          459 a         1212 c         1263 g          563 t
ORIGIN      
        1 gacccgtctc tacgcgtcgg ggcgaatatg gtatagtccc gcggtgatgg aagtcaccgc
       61 ccctcagtca tcaatgccat cgggcgcggc cacggacgtc cgcctctccc gcctggcctg
      121 ggccgtgacc gccgccgggc tggctctgct gggcggcgcg gtcgttttga tcctgctgcg
      181 ggccacgcgc gccccccagg caggggatgt cccctggtac aacgacctga tcgtcaccgc
      241 ctcgctggcc gtggccatcg tcgtcggcgg ctttgtggcc gcgcggttgc cgcgcaaccc
      301 ctacggctgg ctgctgctgg ctttcggcat cggcaacggc agcatccaga gcttcgccgt
      361 cgattacatc ctcgtcagcc atcagatcac gccgctgccg ctgacttcgt gggctttcta
      421 cttcgccgcg ttcggcttct cgctgtggtt gtcggccatt ccgctgctgt ttctgctctt
      481 tcccagcggg cggctgccgt cgcggcgctg gcgctggctg gcggccgttg tcgtcctcag
      541 tttcctgacg ctggcggcgc tgatgtggct atcgccgtcg gcgattctcg tgtccgtccc
      601 cagcccgttc caccacgaca acgccctcgg ccgctgggcc gacaatctga ccagcacagc
      661 cgtcttcttt atcctgctgg ccgtactcat cagcgccatt tccatcgtcg tccgggccat
      721 ccgcgcgcgg gggctggagc gtcagcagtt caaatggctc ggcctggcct cggtggtgat
      781 ggtgctggcc ttcttcttca acaccgagct ggtgccgctg ctgccgggcg tgttcgacgc
      841 gctgctggag gcggccgcct ttgccggcgt gcccctggct gtgggcattg ccgtgctacg
      901 ctaccggctg tgggacatcg acgtcattat ccgcaagacg gcgcagtata gcgtgctatc
      961 gactttgctg gctgtggtct tctacgtact ggtcatcgtc ttacaggagg cctttggccg
     1021 ggtcaccgag agcgactcgc ccgcggccgt cctcctgtcg acgctggcca tcgccgcgct
     1081 gttcgcgccg ctgcgccggg cggtgcagaa cgtcatcgac cgccgcttct accgccgcaa
     1141 ggtcgacgcc gagaaggtgc tggccgactt tgccgccacc gtccgcgacg agaccgacct
     1201 cgaccgcctg acggccgagc tggcgcgcgt catccaggaa gcgatgcagc cggagcacgt
     1261 cagcgtctgg ttgcgggagc cggctggcgg ccggcgggcg acggccgagg gccgcgcggt
     1321 ggcaacgagc gacgagacgc gcgtcgccta gcccggcgct tcgtccggcg ggcgaatgtg
     1381 ggcgaggagc gcgtcagcgt caacgctgtc gccaggggcg tagtggatgg cctcgacaat
     1441 gcccgcggcg gccgtgcgga tggtgtgctc catcttcatg gcttcgatct tcaccaacgc
     1501 ctgtccctcc tccacgcgct gcccgacggc caccagcacg gccagcaccg tgcccggcag
     1561 tggcgcgcgc agggagccgg cggcgtcgac ggcgcggtgg ggttctggcc agaggggcag
     1621 cgccgtcagt cgcagcggcc cgtcgtcggt ctgcgcccac cacgtgtcgc ccgcgttggc
     1681 cagcgccaca tggcggcgcg cgccgtcgat ggtcagagtt aggttggggg ccgcgtcctc
     1741 ttcgagggtg acgaggcgtg gggaagcgtc gccaggcagg gtgacgtggt attgccccgt
     1801 cgtccaggga gtgggtcgca ggcggacatc gactgtctcg ccgccgagac gatagcgaaa
     1861 aggagccggc tcgccggggt tgttgcgcca atagcccggc ccggcggcgc tcagccggcg
     1921 ccagcgggcc acagtgacgg caatgagggc cacggccgac gcctccgcgc tgaccgccgg
     1981 ccgccagtcg ggcagctcat cctccaggaa gtgggtgctg agggcggccg ggctggcgaa
     2041 agcagggcga gtgacgatgg cgcgcagata ggccaggttg gtcgtcaggc cgagcaggac
     2101 agtgtgggcc agggcgcgct ccagccggcg cagggcgtcg gcgcgcgtcg gcccggtggc
     2161 gatgagcttg gccaatagcg ggtcgtagtg gctggtgacg acgctaccgg tgcggatgcc
     2221 gtcgtcgacg cgcacgccct cgctcgcggc cggctgccac agcagcaccg tgccggtgtc
     2281 gggcaggaag tcggccgccg ggttctcggc gtagagtcgc gcctcgacgg cgtggccggt
     2341 cggggcgatg ccggcctgtg accagggcag tggctcgccc gcggccacgc gcagctgcca
     2401 ctcgaccagg tccaggccgg tgaccatctc cgtcaccgga tgctcgactt gcaggcgcgt
     2461 gttcatttcc aggaaggcga agcgaccggc ctcgtcgagc aggaactcga ccgtgcccgc
     2521 gccggtgtag ccgatggccg cggccaggcg gacagcggcc gcgcccatct ctgcccgtag
     2581 gccgtcgctc agaccgggcg cgggcgattc ctcgatgacc ttctggtggc ggcgctggac
     2641 gctgcaatcg cgctcaccca ggtggagcgt gtggccgtgg ctgtcggcca ggacctgcac
     2701 ctcgacgtgg cgcgggcgca ggacggcgcg ctccagcagc aggcgaccgt cgccaaaggc
     2761 cgccgtggcc tcgcgccgcg ccccggccag cgccgccggc aggtcatccg gccgggccac
     2821 cagacgcagg cccttgccgc cgccgccggc agcggccttg accatcagcg gatagccgac
     2881 gcgggcggcc tcggaggcca gcgtcgcgtc gtcgtcggcc tcgccgtcgt agccggccag
     2941 cacgggcacg cccaacccgg ccgccagccg gcgcgcggcg cgcttgtcgc ccatcgcggc
     3001 gatggcctcg gccggcgggc cgacgaagac cagcccggcg gcgacgcagg cgcgggcaaa
     3061 ggcggcgttc tcggccagga agccatagcc ggggtggaca gcgtcggcgc cggctcgcgc
     3121 ggcggcgtcg agcagagcgg ggatgttaag gtaggactcg gccgcggccg ccgggccaag
     3181 gcgcacagcc agatcggcct cggccacgtg ggggctgtcc acgtcggcgt cggagtagac
     3241 ggccacggcg cggatgccca gccgccggca agcgcggatg atgcggcggg cgatttcgcc
     3301 gcggttggcg atgaggaggg tgtcaaagtg gcaaatactc aatcttgtcc ctgaactctt
     3361 gggctgttac cgtcgcggcg ataagcaaaa ggtcttcgat ggactgaccc aaggcggccg
     3421 attgagagac ttcgatcacc tcggcaatct ctcctcccga cgccatgcgc tcataggcga
     3481 tccgcgtcac cgtgctc
//
LOCUS       DAOSMQ010000341         3497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26953_length_3497_cov_4.364033, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000341 DAOSMQ010000000
VERSION     DAOSMQ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26953_length_3497_cov_4.364033"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..>3497
                     /locus_tag="PK829_10900"
     CDS             331..>3497
                     /locus_tag="PK829_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU41762.1"
                     /translation="MTKRSLRLTTLAAVLLLGLLLAGVVVAAPTSVTIAGSFQSELGC
                     TGGSAGDWDPACAITHLTYDAGDDVWQGTFNVAAGPQEYKAALNDDWAESHPANNLAL
                     TGTGDPVKFYYDDKTKFVSDSTRLIIVAVGDWQDQIGCSGTWAPDCLRGWMTDPDNDG
                     TYTFETEAIGPGNWEAKGATGESWSNPNFPSSNYLFTVPAGGGRVLFSFVDATDTFTV
                     TVTPLGHGHDNNIEYDGLGHNSHDTLYRVPFGAVTPGTDVILRFRTYHNDVESVRARV
                     WDDAAQGQFFLDFDLAAAGVSCYDEAQPAETCDFWQATLTPTAPTTYYYRFIITDGTA
                     TANYEDDAFRDGGWGEARPNFNDWGYVIQVYDPNFTPIAWLQNAVMYQIFPDRYFNGK
                     TANDPNTNLPRYGWPPEPLDRIIRKAWNTLPEGYCRFYVNPAQPCTETPRGRDYFGGD
                     LAGVTKRISYLKSLGVTAIYFNPIFDAASNHAYDTQDYLRIDPFFGTNQEFNQLVKAA
                     NKAGIRIILDGVFNHVSSDSKYFDRYHHFSDLGACESVSSPYRSWFTFTPQAGGPCAG
                     PGGPNTMNYSAWFGFDSLPVLNKNVQGVRDLFYAGPNSVNDTWLKRGAAGWRLDVMGD
                     GSFPASFWQEFRVDVKAINPNAPIIGELWKKFEILPKVRGDQADTAMGYRFRNAILGF
                     FGRVDDKGFNDDGQTDQPPSLFASKLVSIREDNPDASYYTMMNLMGSHDTQRILWLLT
                     PGARNREAREFNAANLAQGKTLLKLAAVVQMTTPGTPTIYYGDEIAMTGDDDPDDRRP
                     FPWNGNQPGGDANMLAHYQLLTSLRNSNKVFRQGEMTFLLTDDAARTVAYLMRTNTEA
                     AVVAINRNATAQTLVIPATGHLPNAVMLTDKLGTLPGTITAAGGVITIELPAQSAAIL
                     MPNRGQDLVPPRAAANLTAEAGNGQVVLHWNAPAGGAASYNVYRSPVTGGGYVLIGNT
                     TATTYTDMTVENGREYFYVVRAVDAAGNEGPACNEAVATPFFPITYAVLQWPPTMNFP
                     VRTSGSDTVYGRVYVAGVTDMGGDP"
BASE COUNT          655 a         1285 c         1023 g          534 t
ORIGIN      
        1 ccttgacctc tcccccaaac ctgctaaaac accatcacat ctgctcgcgg ccgcctggcc
       61 gcgctgccca aatcgcacct ttagcttaac ggcattcacc gtagctacag cgagaacgcg
      121 ccgccccagg ggtatcagga aggacggcgg cgcgcccggc aaggatggga agctgtccgg
      181 gaggtgatcg gcgaccaaca gacacaattc accctgcttc tctttcccag cggcgcgacc
      241 cgcgcggccg ctggttccgt tgcgtattgt cactgtgctg catacgacgg ctccgctgct
      301 gcgctggagc cggtgaggag tctacttccc atgaccaaac gatccttgcg cctgacaacc
      361 ctggcagccg tgctgctgtt gggcctgctg ctggccggcg tcgtcgtcgc cgccccgacc
      421 agcgtcacca ttgccggcag cttccagagc gagctgggct gcaccggcgg ctccgctggc
      481 gactgggacc ccgcctgcgc catcacccac ctgacctatg acgccggcga cgacgtctgg
      541 cagggtacat tcaacgtcgc cgccggcccc caggagtaca aggccgccct gaacgacgac
      601 tgggccgagt cgcacccggc caacaatctg gccctgaccg gcacgggcga cccggtcaaa
      661 ttctactatg acgacaagac caagttcgtc agcgatagca cgcggctgat catcgtcgcc
      721 gtgggcgact ggcaagacca gatcggctgt agcggcacct gggcgcccga ctgcctgcgc
      781 ggctggatga ccgaccccga caacgacggc acctacacct tcgagaccga ggccatcggc
      841 cccggcaact gggaggccaa aggcgcgacg ggcgagagct ggtctaaccc caacttcccc
      901 agcagcaact acctcttcac cgtgccggcc ggcggcgggc gggtgctgtt ctccttcgtg
      961 gacgcgaccg acactttcac cgtcactgtc accccgctgg gccacggcca cgacaacaac
     1021 atcgagtatg acggcctggg ccacaacagc cacgacacgc tctaccgcgt gcccttcggc
     1081 gcggtgacgc ccggaaccga cgtcatcctg cgcttccgca cttaccataa cgatgtcgag
     1141 agcgtccgcg cccgcgtctg ggacgacgcc gcgcaggggc agttcttcct cgacttcgac
     1201 ctggccgccg ccggcgtgtc ctgctacgac gaagcgcaac cggccgagac gtgcgacttc
     1261 tggcaggcga cgttgacgcc caccgcgccg acgacctatt actaccgctt catcatcacc
     1321 gacggcacgg ccacagccaa ctacgaggac gatgccttcc gcgatggtgg ctggggtgag
     1381 gcgcggccga acttcaacga ctggggctac gtcatccagg tctacgaccc caacttcacg
     1441 cccatcgcct ggctgcaaaa cgcggtcatg taccagatct tccccgaccg ctacttcaac
     1501 ggcaagacgg ccaacgaccc caacaccaac ctgccgcgct acggctggcc gccagagccg
     1561 ctggatcgta tcatccgcaa ggcgtggaac accctgccag agggctattg ccgcttctac
     1621 gtcaacccgg cccagccctg caccgagacg ccgcgcggcc gcgactactt cggcggcgac
     1681 ctggccggtg ttaccaaacg catcagctat ctcaagagcc tgggcgtgac ggcgatctac
     1741 ttcaacccca tcttcgacgc cgccagcaac cacgcctatg acacgcagga ctacctgcgc
     1801 attgacccat tctttggcac aaaccaggag ttcaaccaac tcgtcaaggc tgccaacaag
     1861 gccggcattc gcattatcct tgacggcgtg ttcaaccacg tctcgtcgga tagcaagtac
     1921 ttcgaccgct accatcactt cagcgacctc ggcgcgtgcg agagcgtctc ttcgccctat
     1981 cgcagctggt tcacctttac gccgcaggct ggtggcccgt gcgccgggcc aggcgggccg
     2041 aatacgatga actacagcgc ctggttcggc tttgacagcc tgccggtgtt gaacaagaat
     2101 gtgcagggcg tccgggacct gttctacgcc gggccgaact cggtcaacga cacctggctg
     2161 aagcgcggcg cggccggctg gcggctcgac gtgatgggcg acggcagctt cccggccagc
     2221 ttctggcagg agttccgcgt cgacgtcaag gccatcaacc ccaacgcgcc gatcatcggc
     2281 gaactgtgga agaagttcga gattctgccc aaggtgcgcg gcgaccaggc cgacacggcc
     2341 atgggctacc gcttccgtaa cgccatcctc ggcttctttg gccgggtcga tgacaagggc
     2401 ttcaacgacg acggccagac cgaccagccg ccgtcgctct tcgccagcaa gcttgtctcc
     2461 atccgcgagg acaaccccga cgccagctac tacaccatga tgaacctcat gggtagccac
     2521 gacacgcagc gcatcctctg gctgctgacg cccggcgcgc gcaaccgcga ggcgcgcgag
     2581 ttcaacgccg ccaacctggc ccagggcaag acactgctga agctggccgc cgtggtgcag
     2641 atgaccacgc ccggcacgcc gacgatctac tacggcgacg aaatcgccat gaccggcgac
     2701 gacgaccccg acgaccggcg gccgttcccc tggaacggca accagcccgg tggcgacgcc
     2761 aacatgctgg cccactacca gctgctgacc agcctgcgca acagcaacaa ggtcttccgc
     2821 cagggcgaga tgaccttcct gctgaccgac gacgcggcgc ggacggtggc ctacctgatg
     2881 cgcaccaaca ccgaggcggc cgtcgtggcc atcaaccgca acgccacggc gcagacgttg
     2941 gtcatccccg ccaccggcca tctgcccaac gcggtcatgc tgaccgacaa gctgggcacg
     3001 ctgccgggga cgatcaccgc cgcgggcggc gtcatcacca tcgagctgcc cgcccagagc
     3061 gcggccatcc tgatgcccaa ccgtggccag gacctcgtgc cgccgcgcgc ggccgctaat
     3121 ctaaccgccg aggccggcaa tggtcaggtt gtcctccact ggaacgcccc cgccggcggg
     3181 gcggcgtcct ataacgtcta tcgcagcccc gtcaccggcg gcggctacgt cctgatcggc
     3241 aacaccacgg ccacaaccta caccgacatg acggtggaaa acggccgcga gtacttctac
     3301 gtcgtccgcg ccgtcgatgc cgctggcaac gaggggccgg cgtgcaacga ggccgtggcc
     3361 acgcccttct tccccatcac ctacgccgtg ctccagtggc cgccgacaat gaacttcccg
     3421 gtgcgcacca gcggttcgga caccgtctac ggccgcgtct acgtggccgg cgtgactgac
     3481 atgggcggcg acccgaa
//
LOCUS       DAOSMQ010000342         3493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_26990_length_3493_cov_3.844095, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000342 DAOSMQ010000000
VERSION     DAOSMQ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26990_length_3493_cov_3.844095"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /locus_tag="PK829_10905"
     CDS             complement(<1..1870)
                     /locus_tag="PK829_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41763.1"
                     /translation="MPAWLVVLLLPAAGFVPGWLVVRQFDRSRLSLPAAFAALTLGLA
                     ALGWVALLLAEAGAFAVGWLAAVWALLVVSLLVVDRWRSRASRTPMMLTGRPAAPRLF
                     PALPAWLEVAFLAAWVVVAGWLFFRPHEYVLGAADAGVYVSLGASIAREGSIVVPDET
                     LAGLDMALYDTLLRPMSDNPIAPWYLLPGFYVVGQPPGEITPQFYPLHPVWLAVAFGL
                     VADTSSPPATAEAIRAELLMNGLWATLGALAVYFTVRQFAGWETAALALVALSLTGLQ
                     VWFARYPTTEVLTQYMLWSGLFGLGHWLGGDRPAKLWALVAGVALGMAFLVRVDLLVL
                     LPVLTLLALWLLAGGPRAAAPGTTGWFLAPLGGLVAHSFAHAWLQSRPYFVVHSGLGL
                     RLLQVNWAIPVVAALAAVAFLGLLRRAGPQFLAGYARYRRAALLALIGVTLVFAVYGW
                     FIRPVVGAPAVQADLFSGGEITLTDHENWRRLGWYLSPVGVWLGVAGTCLLLWRANRR
                     TAMVLAIGALFAALYLWTLYANPQHVYVMRRMVPAAVPFFTVGGAAVVGWLAGSGGRG
                     GARLALAAAVALVWLGGLAWSARGLVSQVDHRGLTAQLDALNAALEPDAVLLFYDGV"
     gene            complement(1873..3417)
                     /locus_tag="PK829_10910"
     CDS             complement(1873..3417)
                     /locus_tag="PK829_10910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU41764.1"
                     /translation="MNGERFDKARLDELGRRAELSEYTFASTAPGVGRLVAGLRAAWN
                     DVATRWHVRPLAAQQSAFNTALVDWLAHRQLEPGDVDTRRIALDRAGAQLNRDTAAVT
                     ARAARLGGDLRARGVGGGAILPNRIRRRWRLAYFSPLPPARSGIADYSAELLPYLAER
                     ADITLFTDAPDAPGDLPRRPTAAFPAERWAYDLPFYQMGNSAHHAAIYHMLRRYPGVT
                     VLHDYVLHHLMAELTLGAGSFAAYAREMGYDLGEAAAPLLRDIRSGRAANPLTEVALN
                     ARVIDSSLGLIVHSHTVAEWIRAVRPAARVAVVPHLVAPRHTHSRRPELHLPPEALLV
                     AIVGQVTTTRQLPLALRVFRRLLDDVPDARFLIVGQVLPEVDLDGLLAELNLGESVIR
                     LDYVESFDAFNDWAATADIVLNLRYPTLGETSGAALRVMAAGRALVVFDHGWYAEIPD
                     GAAVKIPPLDEEALLAALLALARSPERRAALGAAAARYVAEVCAPAAVAGVILDFIAA
                     LEPAGA"
BASE COUNT          596 a         1247 c         1274 g          376 t
ORIGIN      
        1 gcacgccatc gtagaacagc agcaccgcgt ccggctccag cgcggcgttg agcgcgtcga
       61 gttgcgccgt caggccgcgg tgatcgacct ggctgacgag gccgcgcgca gaccaggcca
      121 gcccgcccag ccagaccagc gccacggccg cggccagggc caaccgcgcc ccgccccgac
      181 cgcccgaccc ggccagccag ccgacgacag ccgccccgcc cacggtgaaa aagggcacgg
      241 ccgccggcac catccggcgc atgacgtaga cgtgctgcgg attggcatag agcgtccaca
      301 gatagagcgc ggcgaagagc gcgccgatgg ccagcaccat cgccgtgcgc cggttggcgc
      361 gccagagcag caggcacgtt ccggctacgc ccagccagac gcccaccggc gacagatacc
      421 agcccagccg ccgccaattc tcgtggtcgg tcagcgtgat ctcgccgccg ctgaacaaat
      481 cggcctgcac ggccggcgcg ccgacgacgg gccggatgaa ccagccgtac acggcgaaaa
      541 cgagtgtcac gccaatgagg gccagcaggg cggcgcggcg gtagcgggca tagccggcca
      601 ggaactgcgg cccagcgcgg cgcagcagcc ccaggaaagc caccgccgcc agcgcggcca
      661 cgacgggaat ggcccagttc acttgcagca ggcgcagccc cagcccagag tggacgacga
      721 agtagggccg gctctggagc caggcgtggg cgaacgagtg ggccaccagc ccgcccagcg
      781 gcgcgaggaa ccagccggtc gtgcccggcg cggctgcccg cggcccgccc gccagcagcc
      841 acagagccag cagggtcagc accggcagca gcaccagtag atcgacgcgc accaggaagg
      901 ccatccccag cgctacgccg gccaccagcg cccagagctt cgccggccgg tcgccgccca
      961 gccagtggcc cagaccaaac aggccgctcc agagcatata ctgcgtcaac acttcggtcg
     1021 tcgggtagcg ggcaaaccag acttgcagcc ccgtcagcga cagggcgacg agagccagtg
     1081 cggctgtctc ccagccggcg aactggcgca cggtgaagta gaccgccagc gccccgagcg
     1141 tcgcccacag accgttcatg agcagctcgg cgcggatggc ttcggccgtg gccgggggag
     1201 aagacgtatc cgccaccagc ccgaaggcga cggccagcca gaccgggtgc agcgggtaga
     1261 attgcggcgt gatctcgccc ggcggctggc caacgacgta aaagccgggc aggaggtacc
     1321 acggggcgat ggggttgtcg ctcatcggcc gcaacagggt gtcgtagagg gccatgtcca
     1381 gcccggccag cgtctcgtcc ggcacgacaa tgctaccctc gcgggcgatg gacgcgccca
     1441 ggctgacgta gacgccggcg tcggccgcgc cgaggacgta ctcgtgtggc cggaagaaga
     1501 gccagccggc gacgacgacc cacgccgcca gaaaggcgac ctccagccac gccggcagcg
     1561 ccgggaagag gcgcggcgcg gccgggcggc cggtcagcat catgggcgtg cggctggcgc
     1621 ggctccgcca ccggtcaaca accagcaggc tcacgacgag cagcgcccag acggccgcca
     1681 gccaccccac ggcgaacgcg cccgcctcgg ccagcagcag tgccacccag cccagcgccg
     1741 ccagaccgag ggtcagcgcg gcaaaggccg ctggcaggct gagccggctg cggtcgaact
     1801 ggcgcacgac cagccagccg ggcacaaagc cggccgccgg caacagaaga acgacgagcc
     1861 aggcgggcat ggctacgccc cggccggctc cagcgcggcg atgaagtcga ggatgacgcc
     1921 ggccacagcc gccggagcgc agacctcggc cacgtagcgc gccgcggccg cgcccagcgc
     1981 cgcccgccgc tccggcgacc gggccagcgc cagcagcgcg gccagtagcg cttcctcgtc
     2041 caacggcggt atcttgactg cggccccgtc gggaatctcg gcgtaccagc cgtggtcgaa
     2101 gacgaccagc gcccgcccgg cggccatgac gcgcagggcc gcgcccgatg tctcgcccag
     2161 ggtcgggtag cgcaggttga ggacgatgtc ggccgtggcc gcccagtcat tgaaggcgtc
     2221 gaatgactcg acataatcca gccgaatgac gctctcgccc aaattcaact cggccagtag
     2281 cccgtcgaga tcgacttcgg gtagcacctg gccgacgatc agaaagcgcg cgtcgggcac
     2341 gtcgtccagc agtcgccgga agacgcgcaa ggccagcggc agttgtctgg tcgtcgtcac
     2401 ctggccgacg atggccacga gcagcgcctc cggtggcaga tgtagctccg gccggcgcga
     2461 gtgggtgtgg cgcggggcga caaggtgggg gacgacggcc acgcgcgccg ccggccggac
     2521 ggcgcgaatc cattcggcca ccgtgtggct gtggacgatg aggcccaggc tgctgtcgat
     2581 cacccgcgcg ttgagggcta cttcggtcag cgggttggcg gcgcggccgc tgcggatgtc
     2641 gcgcagaagc ggcgcggcgg cctcgcccag gtcatagccc atctcgcggg cgtaggcggc
     2701 aaagctgccc gcgccgaggg tcaactcggc catgaggtgg tggagcacgt agtcgtggag
     2761 cacggtcacg ccggggtagc gccgcagcat gtggtagatg gcggcgtggt gggcgctgtt
     2821 gcccatctgg tagaagggca ggtcataggc ccagcgctcg gccgggaagg cggccgtggg
     2881 gcggcggggc aggtcgcccg gcgcgtccgg cgcgtcggtg aacagggtga tgtcggcccg
     2941 ctcggccagg tagggcaaca gctcggcgct gtagtcagcg atgccgctgc gcgccggcgg
     3001 cagggggctg aagtaggcca gccgccagcg ccggcggatg cgattcggga gaatcgcacc
     3061 accgcccacg ccccgcgccc tcaaatcgcc gcccagccgc gcggcgcggg cggtcacggc
     3121 ggccgtatcg cggttgagtt gcgccccagc acgatcgagg gcaatgcgcc tggtgtcaac
     3181 gtcgccaggc tctagctgcc ggtgcgccag ccaatcgacc agggcagtgt tgaaggcgct
     3241 ctgctgggcg gccagcgggc gcacgtgcca gcgcgtggcc acgtcgttcc aggccgcgcg
     3301 cagcccggcc accagccggc caacgcccgg cgcggtcgaa gcgaaggtgt actcgctcag
     3361 ttcggcgcgg cggcccagtt cgtccagtcg ggccttgtcg aagcgttcgc cgttcatcgg
     3421 tggggcgtgc ctgccgggcg gcgcggacta gttgcgccag ggcaaatcgg gcacggccgc
     3481 gggctgggca tag
//
LOCUS       DAOSMQ010000343         3491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27016_length_3491_cov_3.270664, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000343 DAOSMQ010000000
VERSION     DAOSMQ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27016_length_3491_cov_3.270664"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PK829_10915"
     CDS             <1..1075
                     /locus_tag="PK829_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41765.1"
                     /translation="DIVGRPAGDPELLAARAAAHAAPPIATILDAMHPDGYWEKPGPG
                     YGPKYRSTVWALITLGQVGATAAADPRIGRAAAYLLDHTYRPGGQLCAWGGAGASTTA
                     DCLQGNLLAALLSLGIADPRLENAFEWMARSVTGAGVAPASDKHAARRYFASGKSGPL
                     FECAINGHLPCAWGGAKVLLAFGLLPAARRTPLIDDAIHQGVEYILSLDPATGEYPHP
                     YSPKPSGNWWKFGFPVFYVADLLQIVEALAALGYGDDPRLANTRALIAGQADAAGRWP
                     LAYHYNGKMGAGVEFGRKGQANKWVTIRALRALAGQRVDAAARVGAAARVDAAARVDA
                     AARVDAAARVDAAARVDAAARVA"
     gene            1192..1491
                     /locus_tag="PK829_10920"
     CDS             1192..1491
                     /locus_tag="PK829_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41766.1"
                     /translation="MDAYESDSVEGNEVTADLLAFIVDNMGIEDVKGLAFDIGIDPHT
                     LGGALAGELARSLVQTMAQRGELTLLDRALRDLLPAEYDLAFGDALRRSADIDTE"
     gene            1555..2259
                     /locus_tag="PK829_10925"
     CDS             1555..2259
                     /locus_tag="PK829_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU41767.1"
                     /translation="MFDLIAFDADDTLWHNEDLYRDARAQFDRLLAPYDPPPDLEDII
                     HATEMANLPFFGFGVNSFTLSLIETAVRVTGGRVGGQAIGRLIDVAKGMIAADIRLID
                     EVEDTLAALSADYSLLLITKGDLLHQQDKVARSGLGRYFSGVEVVSDKTAATYADILR
                     RRGVAPARFLMIGNSLRSDIVPVAELGGQAVYVPIENTWVYDHVPVPAGLPGAVHQVA
                     RLGEVVALVRQLAGAA"
     gene            complement(2285..>3491)
                     /locus_tag="PK829_10930"
     CDS             complement(2285..>3491)
                     /locus_tag="PK829_10930"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOU41768.1"
                     /translation="PEYSPLRASLFQRWMLIWHAVYIGGLLLALGAALWVARGAWGWR
                     EAALAGIVAALLAGYWALFVREVKWPHPNWLLALYFAGVLALLALAAWLNGLFVYVIG
                     MLFGQMFALLPPALALPGVAAVLGVILLAANDWRLPADVSWLSALFVAAQLGLMYLLY
                     LYIYHVFRTSQERAGLVNELRAANEQLARAEAEGRELAALRERERMARDLHDGLGHSL
                     AALSVHLEAVQRLYPVDPERASAQIDAMKRLTRDSMAELRRALDDLRAPADGGEPLAA
                     ALERLCAGLAERTGMVVERRIDTAALAAPPAVAEALRRVAGESLANVERHAGATRVQV
                     ELRAEPGVVVLRVADDGRGLPADAEARPGHYGLRGMRERVEGLGGTLSLGGAGGATVV
                     ARLPVVGRA"
BASE COUNT          538 a         1256 c         1242 g          455 t
ORIGIN      
        1 cgatatcgtt gggcggccgg ccggcgaccc cgaactgctg gccgcccgcg ccgccgccca
       61 cgccgcgccg cccatcgcca ccatcctcga cgccatgcac cccgacggct attgggagaa
      121 gcccggcccc ggctatggcc ctaaataccg ctcgaccgtc tgggcgctca tcaccctggg
      181 gcaggtgggc gcgacggcgg cggccgaccc gcggatcggc cgcgccgccg cctacctgct
      241 cgaccatacc taccggcccg gtggccagct ctgcgcctgg ggcggggccg gggcatcgac
      301 cacggccgac tgcctgcaag gcaacctgct ggccgcgttg ctcagcctgg gcattgccga
      361 tccgcggctg gagaacgcct tcgagtggat ggcccgcagc gtgaccggcg cgggcgtcgc
      421 cccggccagc gacaaacacg ctgcccggcg ctacttcgcc agcggcaagt cggggccgct
      481 gtttgaatgt gccatcaacg gccatctgcc ctgcgcctgg ggcggggcca aggtgctgct
      541 ggcttttggc ctgctgccgg cggcgcgccg cacgccgctc atcgacgacg ccatccacca
      601 gggcgtggag tatatcctca gcctcgaccc ggccacgggc gagtatcccc acccctatag
      661 cccaaagccc agcggcaact ggtggaagtt cggcttcccg gtgttctacg tggccgatct
      721 gttgcagatc gtcgaagcgc tggcggcgct gggctatggg gacgacccgc ggctggccaa
      781 cacgcgggcg ctgatcgccg gccaggccga cgccgccggg cgctggccgc tggcgtacca
      841 ttacaatggc aagatgggcg ctggcgtcga gttcggccgc aaggggcagg ccaacaagtg
      901 ggtgacgatc cgggcgctac gggcgctggc ggggcagcgc gtggacgcgg cggcccgcgt
      961 gggcgcggcg gcccgcgtgg acgcggcagc ccgcgtggac gcggcggccc gcgtggacgc
     1021 ggcagcccgc gtggacgcgg cagcccgcgt ggacgcggca gcccgcgtgg cttaggaacg
     1081 acggccgcgg cacaacgacg gccgtccgca aagatataga ttcgggtaca aatcacgcca
     1141 ggaggattgt ggccgggcga cagccgccta taatggcggt ggagagtgac aatggacgcc
     1201 tacgaaagcg attctgtaga ggggaacgag gtgacggccg acctgctggc cttcatcgtg
     1261 gacaacatgg gcatagagga tgtcaagggg ctggccttcg atattggcat cgacccccat
     1321 acgctgggcg gcgcgctggc cggggagctg gcccgctcgt tggtgcagac gatggcccag
     1381 cgtggcgagt taacgctgct cgaccgcgcc ctgcgcgatc tgctgccggc cgaatatgac
     1441 ctcgccttcg gcgacgcctt gcggcgctcg gccgatatcg acaccgagta gtccccaccc
     1501 cactaccgtg aacctgacag gtggggtcgc ccaccggtca ggtcggaaac gcctatgttc
     1561 gacctcatcg cctttgacgc cgacgacact ctgtggcaca acgaagacct ctatcgcgac
     1621 gcccgcgccc aattcgaccg cctgctggcc ccctacgacc cgccgcccga tctagaggac
     1681 atcatccacg ccacagagat ggccaacctg cctttcttcg gcttcggcgt caatagcttc
     1741 accctgtcgc tcatcgagac agccgtgcgc gtaaccggcg gccgggtggg cggccaggcc
     1801 atcggccggc tgatcgacgt ggccaagggc atgatcgccg ctgacatccg cctgattgac
     1861 gaggtggaag acacgctggc ggcgctgtcg gccgattact cgctgctgct catcaccaag
     1921 ggcgatctgc tccaccagca ggacaaggtc gcccgctccg gcctggggcg ctacttcagc
     1981 ggcgtggagg tggtcagcga caagacggcg gcgacctacg ccgacatcct gcggcggcgc
     2041 ggcgtggccc cggcgcgctt cctgatgatc ggcaacagcc tgcgcagcga catcgtgccc
     2101 gtggccgaac tgggtggcca ggccgtctac gtgcctatcg agaacacctg ggtctacgac
     2161 cacgtgcccg tgcccgcggg gctaccgggc gcggtgcacc aggtggcgcg gctgggcgaa
     2221 gtggtggcgt tggtgcggca gttggccggc gcggcgtaga cagccagcgc ccgacgccgc
     2281 gcggctaggc gcggccgacg accggcagcc gggcgacgac cgtcgcgccg cccgcgccgc
     2341 ccagcgacag cgtgccgccc aggccctcaa cccgttcgcg catcccccgc aggccatagt
     2401 ggccggggcg cgcttcggcg tcggcgggca ggccgcggcc gtcgtcggcc acgcgcagca
     2461 caaccacccc cggctcggcc cgcagctcca cctgcacgcg cgtcgccccg gcgtggcgct
     2521 cgacgttggc caggctttcg ccggccacgc ggcgcagcgc ctcggccacg gccggcggcg
     2581 cggccagagc ggccgtgtcg atgcgccgct cgaccaccat gcccgtccgc tccgccaggc
     2641 cggcgcacag ccgctccagg gccgcggcca gcggctcgcc gccgtcggcc ggggcgcgca
     2701 ggtcgtcgag ggcgcggcgc agctcggcca tgctgtcgcg cgtcagccgc ttcatagcat
     2761 cgatctgggc cgaggcgcgc tcaggatcga cgggatagag gcgctggacg gcctccaggt
     2821 gcaccgacag ggcggccagc gagtggccca ggccgtcgtg gaggtcgcgg gccatgcgtt
     2881 cgcgctcgcg cagcgcggcc agctcgcgcc cctcggcctc ggcgcgggcc aattgctcgt
     2941 tggcggcgcg caactcgttg accagtccgg cccgctcctg gctggtgcgg aagacgtggt
     3001 agatgtagag gtagagcagg tacatcagcc ccagttgggc ggcgacgaaa agcgcgctga
     3061 gccagctgac gtcggccggc agacgccagt cattggcggc cagcaggatg acgcccagca
     3121 cggcggccac ccccggcagc gccagcgccg gcggcagcag ggcgaacatc tgcccaaaga
     3181 gcatgccgat gacgtagacg aagaggccat tgagccacgc cgccagggcc agcagggcca
     3241 acacgccggc aaagtagagc gccagcagcc agttgggatg gggccacttc acttcgcgga
     3301 cgaacagcgc ccagtagccg gccagcagcg cggcgacgat gcccgccagc gccgcctcgc
     3361 gccagcccca cgcgccgcgg gcgacccaaa gggccgcgcc cagcgccagc agcagcccgc
     3421 cgatgtagac ggcgtgccag atcagcatcc agcgttggaa gagggaggcg cggagggggg
     3481 agtactccgg t
//
LOCUS       DAOSMQ010000344         3467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27256_length_3467_cov_4.975967, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000344 DAOSMQ010000000
VERSION     DAOSMQ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27256_length_3467_cov_4.975967"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..2322
                     /locus_tag="PK829_10935"
     CDS             172..2322
                     /locus_tag="PK829_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41769.1"
                     /translation="MNKRIIFLGLLPLIVLIHAGSPRSAAGLARLDSIPPVPLPVWSD
                     EVVATGPTLAGQGRPGMALGVDDKAHIVFGQSQLFHAWPDGATWRFEMVTTTAGPFYS
                     PAIASDASGTLIIVAAAASPTTGEMGLLVVSRAAGGAWQTAAVALPRIDAAPALSLAL
                     DSAGRPHVIVTGRTVDGSPALLYAHASPAGWVSETVAISRAPRGQSVLVLDSQERPVV
                     LYEMGNQPGPGATLSLARRDVAGWHHEVVGAGLILVGKSLALDASDRAHVVFGDNDPT
                     QLVYRWETEDGWQTLIEPGKGFSPDLALDETGRPHLVYVGWDKHLVYATLGDGFWRET
                     WLREAEPAGGDNTLRLDSAGVAHMAVLHEADSLTYITNPGDHWRFDAVAKESATGTPQ
                     ALVLDAADRPHLLAYDATVRQLYWAVKDGDRWTISFVASPPPAGLEIAAVMGPDGVAQ
                     IAYVDSERDQLVAGVRPAGQWSLTPITTAGRGLQLAVGQDNRPHLILFQNDQLNYWTK
                     DNGAWHSEPISPNGVVVTFAFLALDSQGRSKVVYNREGSERLIVAVRQSAGNWATTTL
                     PLRPFLAMALGPDDAVYGLYVTNRSEGVKPPTWFIRLNMAEQVGNDWQITAFNETSPM
                     ADPDGRLLVDAEGRVHVLERNGAEVLTYHQRDENGQWSRDPLPGLGSMALALGSDGQP
                     RVSGILDNDLRFYRRSILWLDEHALLPAVPHPGW"
     gene            2465..>3467
                     /locus_tag="PK829_10940"
     CDS             2465..>3467
                     /locus_tag="PK829_10940"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOU41770.1"
                     /translation="MHFKTIIEPFRIKTVEPIRQTTRAEREQLLREAGYNLFLLPAED
                     VLIDLLTDSGTGAMSSGQWAGMMLGDESYAGAKSFYKFEAAARRITGFKHIIPTHQGR
                     AAERILFGSALKPGDIVPNNTHFDTTRANVEYRHAEARDIPVAEARDPARVLPFKGNI
                     DLAALEQTLSDNAGRVPLAMITVTNNSGGGQPVSMANIRAASEVCHRHGVLFFLDACR
                     FAENAWFIKTREAGYADRTPLEIAQEMFSYADGCTMSAKKDGMANIGGFLALNDDALA
                     ERCKNMLILTEGFPTYGGLAGYDLEAIAVGLEEVLHEDYLQYRVRSVAYLGDILTANG
                     "
BASE COUNT          596 a         1179 c         1114 g          578 t
ORIGIN      
        1 acctcacgca aagatcgcca agggggcaaa gagcgcaaaa aagacgcttc ttggcctcct
       61 ccgcgccctt tgcgtgatgt cttcttccga ctttgcaata gccctggccc tgacaccgct
      121 attgatttac gccgcggctg acctacaatg gtgataggag gcttgggggc catgaacaaa
      181 agaatcattt ttctcggtct gctaccgctg atcgtgctga tccatgccgg tagccctcga
      241 tcggccgcgg gcctggcccg cctggacagc atcccgccgg tccccttacc tgtctggagc
      301 gacgaagtgg tcgctaccgg ccccacgctc gccgggcaag gcaggcccgg gatggcgctg
      361 ggcgtcgacg acaaggcgca catcgttttt ggccagagtc aactctttca cgcctggccc
      421 gacggcgcga cgtggcgctt cgagatggtg acgacgaccg ccggcccctt ctacagcccg
      481 gccatcgcca gtgatgcgtc gggcacgttg atcattgttg ctgcggcggc ctcacccacg
      541 accggcgaaa tggggcttct ggttgtgtct cgcgccgccg gtggcgcgtg gcagacggcc
      601 gcggtcgctc tgccgcgcat tgatgccgcg ccggcgctct cgcttgccct ggacagcgct
      661 ggccggccgc acgtcatcgt cactggtcgc acagtcgatg gctcgcccgc ccttctctat
      721 gcccacgcct cgcccgcggg ctgggtcagc gagacagtgg ccatttcccg cgcgccgcgc
      781 ggccaatcgg tgcttgttct cgacagtcaa gagcgccccg tcgtcctcta tgagatgggc
      841 aaccagcccg gccctggcgc gaccctctcg ctcgcccgcc gcgacgtcgc tggctggcat
      901 cacgaagtcg tcggcgcggg tctgatcctc gtcggcaaat cgctggcgct cgacgccagc
      961 gaccgggcgc atgtcgtctt cggcgacaac gacccgactc aacttgtcta tcgctgggag
     1021 accgaagatg gctggcagac gctcatcgag ccgggcaaag gcttttctcc cgatctggcg
     1081 ctggacgaga cgggccggcc gcaccttgtt tacgtcggct gggacaagca cttggtctac
     1141 gccacgctcg gcgacggctt ctggagagag acctggctgc gcgaagccga gccggccggc
     1201 ggcgacaaca cactgcggct ggattcggcc ggcgtggcgc acatggccgt gctacacgag
     1261 gccgatagcc tgacttacat caccaatccc ggcgatcatt ggcggttcga cgcggtggcc
     1321 aaggagagcg cgacgggcac accgcaggcg ctggtgctgg atgcggccga caggccgcat
     1381 ctgctggcct acgatgcgac cgtgcgacag ttgtactggg cggtcaaaga cggcgacagg
     1441 tggacaataa gctttgtcgc cagcccaccc ccggccggcc tggagatagc cgcggtgatg
     1501 gggccggatg gcgtggccca gatcgcctac gtggacagcg agcgggatca gcttgtcgcc
     1561 ggcgtgcggc cggccggtca gtggtcgctg acgcccatta ccaccgccgg ccgcggattg
     1621 caattggctg tgggacagga taaccggccg cacctgatcc tgtttcagaa cgaccagctc
     1681 aactactgga caaaggacaa cggcgcctgg cacagcgagc cgattagtcc gaacggggtc
     1741 gtcgtcacct tcgccttcct ggccctcgac agtcagggcc ggtccaaggt agtctacaac
     1801 cgcgagggct cagagcgact gatcgtcgcc gtgcgccaga gcgccggcaa ttgggccacc
     1861 acgacgctgc cgctccggcc atttctggca atggcgttgg ggccggatga cgccgtctat
     1921 ggtctttacg tgacgaatcg ctctgagggt gtcaagccgc caacgtggtt catccggctg
     1981 aatatggccg agcaggtggg caacgactgg cagattaccg ctttcaacga gacatccccg
     2041 atggccgacc ccgacggccg cctgctggtc gatgccgagg gccgggtcca cgtccttgag
     2101 cgcaacgggg ccgaggtgct gacctaccat cagcgcgacg agaacggcca atggtcgcgc
     2161 gatcccctgc ccgggctagg aagcatggcc ctggcgttgg gcagcgacgg ccagccgcgc
     2221 gtcagcggca tcctcgacaa tgacttgcgc ttctaccggc gctccattct ctggctggac
     2281 gaacacgcct tgctgccagc cgtgccgcat ccgggctggt agcgcaaagc agggcgcggc
     2341 gagataagtc atctcagcgg gcgcgacgtg gcggcagaac ccggcttctg cccgccgcgt
     2401 cacgccgtgt ggttctggcc gcgacgtggt acgatcccgt caccccactt ttcggagaac
     2461 cgccatgcac ttcaagacca ttatcgagcc attccgcatc aagaccgttg aacccatccg
     2521 ccagacgacg cgcgccgagc gcgaacagtt gctgcgggag gccggctata acctcttcct
     2581 gctgccggcc gaggacgtgc tcatcgacct gctgaccgac tccggcacgg gagcgatgtc
     2641 gtcggggcag tgggcgggca tgatgctggg cgacgagagc tacgccgggg cgaagtcgtt
     2701 ctacaagttc gaggccgccg cccggcgcat caccggcttc aaacacatca ttcccaccca
     2761 tcaggggcgc gccgccgagc gcatcctctt cggctcggcg ctcaagccgg gcgacatcgt
     2821 gcccaacaac acccacttcg acacaacgcg ggccaacgtt gagtaccgcc acgccgaggc
     2881 ccgcgacatc cccgtggccg aggcccgcga cccggcccgc gtactgccct tcaaaggcaa
     2941 catcgacctg gcggcgctgg agcaaacgct gagcgacaac gccggccgcg tgccgctggc
     3001 gatgatcacc gtcaccaaca actccggcgg cggccagccg gtcagcatgg ccaacatccg
     3061 cgccgccagc gaggtctgcc accgccatgg cgtactcttc ttcctggacg cctgccgctt
     3121 tgccgagaac gcctggttca tcaagacgcg cgaggcgggc tacgccgacc ggacgccgct
     3181 ggagatcgcc caggagatgt tcagctatgc cgacggctgc acgatgagcg ccaagaagga
     3241 cggcatggcc aacatcggtg gcttcctggc gttgaacgac gacgccctgg ccgagcgctg
     3301 caagaacatg cttatcctga ccgaaggctt ccccacctat ggcggcctgg cgggttatga
     3361 cctggaggcg atcgccgtgg ggctggagga agtgctgcac gaggactatc tgcaataccg
     3421 cgtgcgctct gtggcctacc tgggcgacat cctgacggcc aacggcg
//
LOCUS       DAOSMQ010000345         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27388_length_3454_cov_3.982348, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000345 DAOSMQ010000000
VERSION     DAOSMQ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27388_length_3454_cov_3.982348"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK829_10945"
     CDS             <1..391
                     /locus_tag="PK829_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41771.1"
                     /translation="TADLSAVAPITTTAQAGPSPTATTAPGTPTAEATATLFPTATPT
                     PTPSPTPSPTPILVPTAVVGENTSTLPVRQLPGGPVLVVLVRGDIVLPRPGHAFQGGD
                     LWREISTVNGVIGWVPDEFLDYGEAAD"
     gene            442..744
                     /locus_tag="PK829_10950"
     CDS             442..744
                     /locus_tag="PK829_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOU41772.1"
                     /translation="MTNVVYTVQNVIFEWDSEKASANWRKHAVSLESAVEAFFDPFLL
                     VVDDEEYVGQELREKVIGMTQDWQVILVVYVMRSDRVRLISARVATGEERRLYENQ"
     gene            731..1030
                     /locus_tag="PK829_10955"
     CDS             731..1030
                     /locus_tag="PK829_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41773.1"
                     /translation="MKINDLQKRLRKERPMITVSFRMPEDVLEDLKRIAPLLGFSGYQ
                     PLMRAYIGQGLRADLARLESSIEVASLVQSLRKQGITEEVIQLALADVHSGASAL"
     gene            complement(1053..2108)
                     /gene="thrC"
                     /locus_tag="PK829_10960"
     CDS             complement(1053..2108)
                     /gene="thrC"
                     /locus_tag="PK829_10960"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU41774.1"
                     /translation="MTSWPGIIEAYRPYLPFDQDTAIVTLNEGNTPLIAAPRLAETLA
                     PGRDFHLFLKFEGLNPTGSFKDRGMTAAITQAVREGAQTVICASTGNTAASAAAYAAR
                     AGLRCLVLVPEGKIALGKLAACLAYGAEVISIDGSFDDGLRLVREISARHPIVLVNSV
                     NPWRLEGQKTGAFEIIDQLGGRAPDWHCLPVGNAGNISAYWLGYKQYGHGLPRLLGGQ
                     AEGAAPIVRGRVVEHPETLATAIRIGNPARWRQAVEALDESDGLITAVSDTDILAAWH
                     LLAKREGVWVEPASATGLAALQQQIAAGAIDPEGQTAVIVLTGHGLKDPGTAVAQAAA
                     PVALPAKLEALEGYLAG"
     gene            complement(2262..3335)
                     /locus_tag="PK829_10965"
     CDS             complement(2262..3335)
                     /locus_tag="PK829_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU41775.1"
                     /translation="MHAKPRPITLLLLLLLLLLLAACARAPQAPAGPNLDLSDPNVGG
                     GAGEALGPETPIPTPTLTREPSPRLDAGAAAAAATADGRPLTAEPLDAAAAAAATADS
                     RQPTAAPLATATAFVVATPLVAATPDAAAPEPTAAATATPAASPTAAGERIHVVQPGE
                     NLYRIGLQYGLSWLAIAEYNGITNPDAISAGQELRIPPSPTATAESTTDLQTTDEAQG
                     SGGAGEQGSRGDGEAATDSGPVVADEPPAGQDDGPPVVAVAAGDTLYAIARQYGVDWA
                     QIAEANGLATPNQLYPGQPLKIPPDVAGPAPAFSHRVHSGETLGRLAAQYGLPADALA
                     AANGLTAPYTIYAGQLLTIPGDE"
BASE COUNT          501 a         1145 c         1219 g          589 t
ORIGIN      
        1 gacggccgat ctgtccgccg tcgcgcccat cactaccacc gcccaggccg gcccctcgcc
       61 cacggccacg accgcgccgg gcacgcccac ggccgaggcc acggcgacgc tcttccccac
      121 ggccacgccg acacccacgc cctcgcccac cccctcgccg acgcccatcc tcgtgcccac
      181 agccgtcgtc ggcgagaaca ccagcacgct gcccgtgcgc cagctgccgg gcgggccggt
      241 gctggtcgtg ctcgtgcgcg gcgacatcgt cctgccccgg cccggccacg ccttccaggg
      301 cggcgacctc tggcgggaga tcagcacggt caatggggtg atcggctggg tgccggatga
      361 gttcctggat tatggggaag cggccgatta gatactgacg cccattgtat tgacaacgta
      421 tatacgtcta gctatcattc catgacgaat gtcgtctaca cagttcagaa tgtcatcttt
      481 gagtgggata gcgagaaggc gtcggccaat tggcgcaagc atgcggtgtc acttgagtct
      541 gcggtagaag ccttcttcga tcctttcctg cttgttgtcg atgacgaaga atacgtcggt
      601 caagagctgc gggaaaaggt catcggcatg acgcaagatt ggcaagttat tcttgtcgtc
      661 tacgtgatgc gcagtgaccg agtgcgactc atttcagcac gggtcgcgac gggtgaggaa
      721 agaaggctgt atgaaaatca atgatttgca gaaacgcttg cgcaaagagc ggccgatgat
      781 taccgtcagt tttcgcatgc cggaggatgt gctcgaagat ttgaagcgta ttgcgccgtt
      841 gctggggttc tccggctatc aaccactcat gcgtgcttac atcggtcaag gcttgcgtgc
      901 tgacctggcc cggctagaga gcagtatcga agtggctagt ttggtacaaa gcttgcgcaa
      961 gcaggggatc accgaagagg tcatccagtt ggcgcttgca gatgtccata gcggtgcttc
     1021 cgccctatga gtgcggcgtc acgtcatacc ccctagcccg ccagataccc ctccaacgcc
     1081 tctaacttcg ccggcaacgc caccggcgcg gcggcctggg ccacggccgt gcccgggtcc
     1141 ttcagcccgt ggccggtcag gacgatgacc gccgtctgcc cctccgggtc gatggccccg
     1201 gcggcgatct gctgctgcaa cgccgccagc cccgtggccg aggccggctc cacccacacg
     1261 ccctcgcgct tggccagcag atgccaggcg gccaggatgt cggtgtcgct gacggcggtg
     1321 atcaggccgt cggactcgtc cagcgcctcg accgcctgcc gccagcgggc ggggttgccg
     1381 atgcggatgg ccgtggccag cgtctccggg tgctcgacga cccggccgcg gacgatgggc
     1441 gcggccccct cggcttgccc gcccagcagc cggggcagcc cgtggccata ctgcttgtag
     1501 cccagccagt aggcgctgat gttgccggcg ttgcccaccg gcaggcagtg ccagtcgggc
     1561 gcgcgcccgc ccagctggtc gatgatctcg aacgcgcccg tcttctgccc ctccagccgc
     1621 cacgggttga ccgagttgac cagcacgatg gggtggcgcg cgctgatctc gcgcaccagg
     1681 cgcaggccgt cgtcgaagga gccgtcgatg ctgatgactt cggccccata ggccaggcag
     1741 gcggccagct tgcccagggc gatcttgccc tccggcacga ggacgaggca gcgcagcccc
     1801 gcccgcgcgg cataggcggc ggcgctggcg gcggtgttgc ccgtgctggc gcagatgacc
     1861 gtctgcgcgc cctcgcgcac ggcctgggtg atggccgcgg tcatgccccg gtccttgaag
     1921 gagccggtcg ggttcaggcc ctcgaacttc aggaacaggt ggaagtcgcg gccgggggcc
     1981 agcgtctcgg ccagccgcgg cgcggcgatg agcggcgtgt tgccctcgtt gagggtgacg
     2041 atggcggtgt cctggtcaaa cggcaggtaa gggcgatagg cctcaatgat gcccggccat
     2101 gatgtcatag tctgttcacc tgtagggaga gaagaagctc acgcaaaggc gccaaggggg
     2161 ccaaggacgc aaagaagaag cagaagcttg ctcttctctc ttcttcttgg tctcttcttc
     2221 ctttgcgcct ttgcttcctt cgcgtccttt gcgtgagcgc tttattcgtc accgggaatc
     2281 gtcaacagtt gcccggcgta gatggtgtag ggcgcggtca ggccgttggc cgcggccagc
     2341 gcatcggccg gcaggccata ttgcgcggcc aggcggccga gcgtctcgcc gctgtggacg
     2401 cgatggctga acgccggcgc cggcccggcc acgtcgggcg gaatcttgag cggctggccg
     2461 gggtagagct ggttgggcgt ggccaggccg ttggcctcgg cgatctgcgc ccaatcgacg
     2521 ccgtactgcc gggcgatggc gtagagcgtg tcgccggcgg ccacggccac gacgggcggc
     2581 ccgtcgtcct gtccggctgg cggctcgtcg gccacgaccg gcccactgtc cgtggccgct
     2641 tctccgtcac ccctgctccc ctgctccccc gctcccccgc ttccctgcgc ctcgtccgtc
     2701 gtctggagat cggtcgtgct ttcggccgtg gccgtgggcg agggcgggat gcgcagctcc
     2761 tgcccggcgc tgatggcgtc ggggttggtg atgccgttgt actcggcgat ggccagccac
     2821 gacaggccat attgcaggcc gatgcggtag aggttctcgc ccggctggac gacgtggatg
     2881 cgctcgcccg cggccgtggg cgaggcggcg ggcgtggccg tggctgcggc cgtaggctcc
     2941 ggcgcggcgg cgtccggcgt ggccgccaca aggggcgtgg ccacgacaaa ggccgtggcc
     3001 gtggccaaag gtgcggcggt cggctgtcgg ctgtcggccg tcgccgccgc cgccgccgcc
     3061 gcgtccaacg gctcggcggt cagcggtcgg ccgtcggccg tcgccgccgc ggccgccgcc
     3121 cccgcgtcca gccgcggcga cggctcgcgg gtgagggttg gcgtggggat gggcgtctcc
     3181 gggccgaggg cctcgcccgc gccgccgccc acgttggggt cggacaggtc gaggttgggg
     3241 ccggccgggg cctggggcgc gcgggcgcag gcggccagca gcagcagcag gagcagcagc
     3301 aggagcaggg tgatggggcg gggcttggcg tgcatcggag gggattatag cagatcgtgg
     3361 gcgaagacct gacaggtggg cggccaaact ctctggtgca aaggaaactt caggccgccc
     3421 acctgtcagg tcagcgcact tttagcgact ttgg
//
LOCUS       DAOSMQ010000346         3452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27421_length_3452_cov_3.330291, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000346 DAOSMQ010000000
VERSION     DAOSMQ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27421_length_3452_cov_3.330291"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PK829_10970"
     CDS             complement(<1..382)
                     /locus_tag="PK829_10970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41776.1"
                     /translation="MSTDSAVATGSPVATTTDHRRDAVWQAEALSNTYLDGVRGAIPL
                     AAEQIDILVRLARAARPAARAILDLGCGDGILGHALLDAYTDTEPTVVLADFSLPMLE
                     AARARLDACANGRSDRVHFTQLDYA"
     gene            complement(399..2045)
                     /locus_tag="PK829_10975"
     CDS             complement(399..2045)
                     /locus_tag="PK829_10975"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOU41777.1"
                     /translation="MSVQPDTVLIFDTTLRDGEQSPGATLNVEEKLEIARQLSRLGVD
                     ICEAGFPIASPGDFEAVRRIAEEVGPLTEGRRDGRPMVIAGLARANETDIGRAHEAVR
                     AAPRHRIHTFLATSDIHLKHKLRIDREECVDQVIKAVTFARSLCDDVEFSPEDAGRSD
                     PDFLVQVLGQAITAGATTLNIPDTVGYTTPDEFGWLIGYLIAHTPGADRVVWSVHCHN
                     DLGLATANTLAGVKAGARQVEVTINGIGERAGNTALEEVVMALSTRPATFNVGSNIDT
                     TQLARTSHMVSTYTGMVVQPNKAIVGANAFAHEAGIHQDGMLKHHLTYEIMRPETVGL
                     NQSQLVLGKHSGRHAFRVHLREMGYDTMDEAQVTAAFRRFKELADKKKIVTDADIEAL
                     IADEIYQPPTIWELEHIQVSCGDHSIPTASVVMIGPGGERYQDAALGAGPVDAVYQAI
                     NRIVGVTNRLTEFSINAVTEGIDAVAETTVRIQPDNEDAFVVNPQTNRPMFRTYSGHG
                     ASTDIVVASARAYLSALNKLLAERQSQAAPHNGNGHAAVA"
     gene            complement(2124..>2906)
                     /gene="ilvC"
                     /locus_tag="PK829_10980"
     CDS             complement(2124..>2906)
                     /gene="ilvC"
                     /locus_tag="PK829_10980"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOU41778.1"
                     /translation="MLAPDTSQASIYRGAIAPNLRPGKTLMFSHGFNIRYGQIIPPAT
                     VDVSMVAPKSPGHRVRELYVEGKGTPGLVAVHQDASGAALATALAYAQAIGCTRAGTI
                     LTTFTEETETDLFGEQAVLCGGVTALVEAAFNTLVGAGYQPEIAYFECLHELKLIVDL
                     MYQGGLSYMRYSVSDTAEHGDYSAGPKIVTEQTRQAMQQILAEIRSGAYAEGWIDENA
                     QGRPWFNQRREEARHSQIEQVGRNLRRMMPWINPKEIMPGEA"
     assembly_gap    2907..2916
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2917..3127)
                     /locus_tag="PK829_10985"
                     /pseudo
     CDS             complement(2917..3127)
                     /locus_tag="PK829_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256075.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            complement(3141..>3452)
                     /gene="ilvN"
                     /locus_tag="PK829_10990"
     CDS             complement(3141..>3452)
                     /gene="ilvN"
                     /locus_tag="PK829_10990"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOU41779.1"
                     /translation="SQTRGEIMQLVSIYHASVVDVDMTSLIIQIVAPEDRVDSLIQLL
                     DHYGIEEMVRTGQVAMVRGSSSHGHSHSVATVAANGQTAPGNGANRNGNGHAEKNGAR
                     A"
BASE COUNT          504 a         1070 c         1203 g          665 t
ORIGIN      
        1 gcgcgtagtc aagctgggtg aagtgaaccc gatcgctgcg gccgttcgcg cacgcgtcca
       61 gccgggcgcg cgcggcctcc agcatcggca gtgagaagtc ggctaggacg accgtcggct
      121 cggtgtcggt gtaggcgtcc agcagggcgt ggccgaggat gccgtcgccg cagccgaggt
      181 cgaggatggc ccgcgccgcc ggccgcgccg cccgtgccag ccgcacgaga atgtcgatct
      241 gctcggccgc caacgggatt gcgccgcgca cgccgtcgag gtaggtgttg ctcagcgctt
      301 ccgcttgcca gacggcgtcg cggcggtggt cggtggtcgt ggccacgggg ctgcccgtgg
      361 ccacggcact gtcggtgctc attgcggtgc tcatcgggtt aggcgacggc ggcatggcca
      421 ttgccgttgt gcggcgcggc ctggctctgg cgttcggcca gcagtttgtt gagcgcgctc
      481 aggtaggcgc gggcgctggc cacgacgatg tcggtgctcg cgccgtggcc gctgtaggtg
      541 cggaacatcg gccggttggt ctgtggattg accacgaagg catcctcatt gtccggctgg
      601 atgcggacgg tcgtctcggc gacagcatca atcccctcgg tgacggcgtt gatgctgaac
      661 tcggtcagcc ggttggtgac gccgacgatg cggttgatgg cctggtagac ggcatcgacc
      721 gggccagcgc ccaacgcggc atcctgatag cgctcgccgc cggggccgat catgaccacg
      781 ctggccgtcg ggatgctatg gtcgccgcag gaaacctgga tgtgttccag ctcccagatg
      841 gtcggcggct gatagatctc gtcggcgatg agcgcctcga tgtcggcgtc ggtgacgatc
      901 ttcttcttgt cggccagttc cttgaagcgc cggaaggcgg ccgtcacctg cgcctcgtcc
      961 atggtgtcat agcccatctc gcgcaggtgg acacgaaagg catgacgacc ggaatgcttg
     1021 cccagtacca actgcgattg gttcaggcca accgtctccg ggcgcatgat ctcgtaggtc
     1081 aggtggtgct tgagcatgcc gtcctggtga atgccggcct cgtgggcgaa ggcgttggcc
     1141 ccgacgatgg ccttgttggg ctggacgacc atgccggtgt aggtgctgac catgtggctg
     1201 gtgcgggcga gttgcgtcgt gtcgatgttg ctgccgacgt tgaacgtcgc cggccgtgtc
     1261 gagagggcca tgacgacttc ttccagcgcg gtgttgccag cccgttcgcc aatgccattg
     1321 atggtcactt ccacctgccg cgcgccagcc ttaacgccgg ccagcgtgtt ggccgtggcc
     1381 agccccaggt cattgtggca gtggacagac cagacgacgc gatcggcccc cggcgtatgg
     1441 gcaatcaggt aacctatcag ccagccgaac tcgtccggcg tggtgtagcc gacggtatcg
     1501 gggatattga gcgtcgtcgc cccggcggtg atggcctgcc ccagcacctg gacgaggaaa
     1561 tcggggtcgg agcggccggc atcctccggc gagaactcga cgtcgtcgca cagcgaccgg
     1621 gcaaaggtga cggccttgat gacctggtca acgcactcct cgcggtcgat acgtagcttg
     1681 tgcttcaggt ggatgtcgct ggtggccagg aaggtgtgga tgcggtggcg cggcgcggcg
     1741 cgaacggctt cgtgggcacg gccgatgtcg gtctcgttgg cgcgggccag tccggcgatg
     1801 accatcggcc ggccgtcgcg tcgcccttcg gtcagcggcc cgacctcctc ggcgatgcgg
     1861 cgcacggcct cgaagtcacc gggggaagcg atggggaagc ctgcctcgca gatatcgacg
     1921 cccaggcgcg acaactgccg ggcgatctcc agcttctcct cgacgtttag cgtcgctccc
     1981 ggcgattgct caccgtcgcg cagggtggtg tcgaaaatca gaaccgtgtc cggttgtacg
     2041 ctcattcaaa acctctttat gatgggggcc aggttccagg gactagtcac tgacctactg
     2101 aatactgacc actgaccatt tccctacgcc tcccctggca taatctcctt ggggttgatc
     2161 cacggcatca tgcggcgcag gttgcggccg acctgctcga tctggctgtg gcgggcctct
     2221 tcacggcgct ggttgaacca tggccggccc tgggcattct cgtcaatcca accctcggcg
     2281 taggctcctg accggatctc ggccaggatt tgctgcatgg cctggcgcgt ctgctcggtg
     2341 acgatcttcg gcccggcgct atagtcgcca tgctcggccg tgtcgctgac actgtagcgc
     2401 atgtagctga gaccgccctg gtacatcaga tcaacgatca attttagctc atggaggcac
     2461 tcgaagtagg cgatctccgg ctggtagccc gcgcccacca gggtgttgaa ggctgcctca
     2521 accagggcag tgacaccgcc acacaggacg gcctgctcgc cgaagaggtc ggtttctgtc
     2581 tcctcggtaa aggtggtcag gatcgtgccg gcgcgggtgc agccaatggc ctgggcatag
     2641 gccagcgccg tggccagggc cgcgccgctg gcgtcctgat gcacggctac caggccgggc
     2701 gtgcccttgc cctcgacgta cagctcgcgg acacggtggc cgggcgactt gggcgcgacc
     2761 atgctgacat cgaccgtcgc cggcgggatg atctggccat agcggatgtt gaagccatgg
     2821 ctgaacatga gggtcttgcc cggccgcagg ttgggggcga tggcgccgcg gtagatgctg
     2881 gcctgcgagg tgtctggggc cagcatnnnn nnnnnncggc gcgctcggcg gtcatcacgg
     2941 ccagcccttc ggcctcgcat ttgctccagg aacggctgcc ttcgtagagg ccgacgcgca
     3001 cgtcgacgcc ggaatcgcgc aggttcaggg cgtgggcgtg gccctggctg ccgtagccga
     3061 tgatagcgac cttcttgccc gccaggcagg cggggtctgc gtctttgtcg tagtaaatcg
     3121 tggccaagat gttgtgctcc ttaggcgcga gcgccgttct tctcggcgtg gccattgcca
     3181 ttcctgttcg ccccgttgcc gggggccgtc tggccgttgg cggcgacggt ggcgacgctg
     3241 tggctgtggc cgtggctcga gctgccgcgc accatggcca cctggccggt gcgcaccatc
     3301 tcttcgatgc cgtagtggtc caacagctgg atcagtgaat ccacgcggtc ttccggggcg
     3361 acgatctgga tgatgagtga ggtcatatcg acatcgacga cgctggcgtg gtagatgctg
     3421 accagttgca tgatctcgcc gcgcgtctgg ct
//
LOCUS       DAOSMQ010000347         3449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27449_length_3449_cov_5.344431, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000347 DAOSMQ010000000
VERSION     DAOSMQ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27449_length_3449_cov_5.344431"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PK829_10995"
     CDS             complement(<1..1316)
                     /locus_tag="PK829_10995"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41780.1"
                     /translation="MDSVRLLDLLEGALSEAEMTALAGALAVAFAAFPGATKRERTGE
                     FLGYVQRQGRSAGLAEALVALRPDLATAVAHLYADNDAQLAWLDQISGHASGHAAPTM
                     SSAVTWRWPADDPGNTREFPLQGDGPDKAVPRPWDPADTRQFPPEKDAPPAAPPNPYL
                     LGRAVSDEAMFFGRDAEQARVQEWLAAGAHVALVGARLFGASSLLRHAARHVVAPWLP
                     AAVDMKEPSVRTRPGLLNAVWGQWWSSVKPSSAAPVNTLAEFVTAARKLNAAGFRPLL
                     FLDELEQLAWRPAVFDDAFFDAWLALGREGAVTFVVTSHVAPAELFAQSGYTSRFYEL
                     FRQLDVGLLDADAARALLTIPIERAGLTVPASAAERLLALAGPQPFFLQLAGLYLYEA
                     LAAEPAAPEAVERHFTAAAAPYWRELWETLSPLAQSAYPVTATPGPR"
     gene            1568..2923
                     /locus_tag="PK829_11000"
     CDS             1568..2923
                     /locus_tag="PK829_11000"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOU41781.1"
                     /translation="MSTFLSFPENFLWGAATASFQIEGAWNEDGKSESIWDRFCHTPG
                     HVKDGSSGDVACDHYHRYPQDVALMKRLGLKAYRFSVAWPRVLPEGRGRVNQAGLDFY
                     SRLVDELLTNDIRPFVTLYHWDLPQVLQDAGGWAARPTAEAFVEYAGAVSAALGDRVH
                     NWITHNEPWCISFLSHMLGIHAPGLRDPYTALRAAHHVLLSHGWAVPVIRANSPGAEV
                     GITLNLEWKDPASRSVADLDATRAADSLANRWFLDPLYGRQYPADGVADFASALPNGL
                     DFVEPGDMAAIAVPTDFLGLNYYTRAVNRAEGDDNLPQVIFPDRPRTDIGWEVYPEGF
                     YKLLNRLHFDYSVPKLYVTENGASYNIGPDAGGRVPDAPRLDYLRRHLAAAHHAIANG
                     VPLAGYFVWSLMDNFEWAEGYTQRFGIVWVDYDTQQRLPKDSALWYGQVIASNGYEHG
                     D"
     gene            2929..>3449
                     /locus_tag="PK829_11005"
     CDS             2929..>3449
                     /locus_tag="PK829_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41782.1"
                     /translation="MNAAPIGANETPPPPSRPGAAYTILLAFWASGMALTIVFALWFS
                     EQVAMAVGGDDWRGTWAAVGVFLAAAVGLPAWVGRRWSLIPRYRAVFGAWLWAAVFVL
                     CLLPARLAGPTAAEAAAGLQIAGLLLYLALVAIVRRRAGVRFASGGRGVAVALFMAAL
                     LALPWVVWGALGSP"
BASE COUNT          534 a         1246 c         1142 g          527 t
ORIGIN      
        1 cgcggccccg gcgtggccgt caccgggtag gcgctctggg ccagtggcga aagcgtctcc
       61 cacaactctc gccagtaggg cgcggcggcg gcggtgaaat ggcgctcaac ggcctccggc
      121 gcggcaggct cggccgccag ggcctcatag agatacagcc cggccagttg cagaaagaac
      181 ggctgcggcc cggccagagc cagcaagcgc tcagccgcgc tcgctggaac ggttagcccg
      241 gctcgctcaa tgggtatagt gagcagcgct cgcgccgcgt ccgcgtcgag caggcccacg
      301 tctagctggc ggaacaactc gtagaagcgc gaggtgtagc ccgactgcgc gaatagctcc
      361 gctggggcga cgtggctggt gaccacaaag gtgaccgcgc cctcgcgccc cagcgccagc
      421 caggcatcga agaaggcgtc atcgaagacc gccggccgcc aggccaactg ctccagctca
      481 tccaggaata gtagcggccg aaaaccggcg gcgttcagct tgcgggcggc ggtgacgaac
      541 tcggccagtg tgttcaccgg cgcggcgcta ctgggtttga ccgacgacca ccactgcccc
      601 cagacggcgt tgagcagccc cggccgggtg cgaaccgacg gctccttcat atcaaccgcc
      661 gccggcagcc agggcgcgac cacgtgccgc gcggcgtggc gcagcagcga cgacgccccg
      721 aacagccgcg cgccgacaag ggccacgtgc gccccggcgg ccagccactc ctgcacacgg
      781 gcctgctcgg cgtcgcggcc gaagaacata gcttcgtcgc tgacggcgcg gccgagcagg
      841 taagggttag gcggagcagc gggcggggcg tctttctccg gtgggaattg gcgcgtgtcg
      901 gccgggtccc acggccgggg cacagctttg tccggcccgt ccccctgaag aggaaactcg
      961 cgcgtgttgc cggggtcgtc cgccggccag cgccaggtga cggccgagga cattgtcggc
     1021 gcggcgtggc cactcgcgtg gccactgatc tgatcgagcc aggccaactg ggcgtcgttg
     1081 tcggcgtaga gatgggccac ggccgtggcc aggtctggcc gcagggccac cagcgcctcg
     1141 gccagcccgg cgctgcgccc ctgccgctgc acatagccca gaaattcgcc cgtccgctca
     1201 cgtttcgtcg ccccgggaaa ggcggcaaaa gccacggcca gcgcgccagc cagggccgtc
     1261 atctccgcct cgctcaacgc gccctccaga agatccagca atcgcactga gtccatcgtc
     1321 ttctcccacg ccggagtata gcgccgatgg ggagacgttc aaccgtggcg cgattctccg
     1381 aatcacagcg ctgcgattcg gagaatcgcg ccacggtctt tcggttgcca cctgccccgt
     1441 ttcgcgtacg atccatccaa ctgcccggcg agctagttcc ccgcgatgga gcacgatcac
     1501 cgggcaacgc tccaagccgt cgctgtcgcc gtccggggca gttgtggcta catcaacgag
     1561 gcaaatcatg tcaactttcc tttcctttcc cgaaaacttc ctgtggggcg cggcgacagc
     1621 atcgttccag atcgaaggcg cctggaacga ggacggcaag agcgaatcga tctgggatcg
     1681 cttctgccac acgcccggcc acgtcaagga cggcagtagc ggcgatgtcg cctgtgacca
     1741 ctaccaccgc tatccgcaag acgtggccct gatgaagagg ctgggcctga aggcctaccg
     1801 cttctccgtc gcctggcccc gcgtgctgcc agaggggcgc ggccgggtca atcaggccgg
     1861 gctggacttc tacagccgcc tggtcgatga gctgctgacc aacgacatcc gcccattcgt
     1921 cacgctctac cactgggacc tgccccaggt gctgcaagac gccggcggct gggccgcgcg
     1981 gccgacggcc gaggcgttcg ttgaatatgc cggcgcggtc agcgccgccc tgggcgaccg
     2041 cgtccacaac tggatcaccc acaacgagcc gtggtgcatc agcttcctga gccacatgct
     2101 gggcattcac gcccccggcc tgcgcgaccc ctacaccgct ctgcgcgccg cccatcacgt
     2161 tctgctgtcc cacggctggg ccgtgcccgt cattcgcgcc aatagccccg gcgcggaagt
     2221 gggcatcacc ctcaacctgg aatggaaaga cccggccagt cgcagcgtgg ccgacctcga
     2281 cgccacgcgc gcagccgaca gcctggccaa ccgctggttc ctcgacccgc tctatggccg
     2341 gcagtacccg gccgacggcg tggccgactt tgccagcgcc ctgccaaacg ggctggactt
     2401 cgtggagccg ggtgacatgg ccgccatcgc cgtgccgacc gacttcctgg gcctcaacta
     2461 ctacacccgc gccgtcaacc gcgccgaggg ggacgacaac ctgccgcagg ttatcttccc
     2521 cgaccggccg cgcacggaca tcggctggga agtgtacccg gagggcttct acaagctgct
     2581 caaccggctg cacttcgatt acagcgtgcc caagctctac gttaccgaga acggcgccag
     2641 ctacaacatc ggcccggacg ccggcggccg cgtgcccgac gcgccccgcc tcgactacct
     2701 gcgccgccac ctggccgctg cccaccacgc catcgccaat ggtgtgccgc tggccggcta
     2761 cttcgtctgg tcactgatgg acaacttcga gtgggccgag ggctacaccc agcgcttcgg
     2821 catcgtctgg gtcgattacg acacccagca gcgcctgccc aaagacagcg ccctctggta
     2881 tggccaggtc atcgccagca acggctacga gcacggcgac tgaccgcgat gaacgcagcg
     2941 cccattggag caaacgagac gccacctccg ccctcgcggc cgggggcggc ctacaccatc
     3001 ctgctggcgt tctgggccag cggcatggcc ctgactattg tcttcgccct gtggttcagc
     3061 gagcaggtcg ccatggccgt cggcggcgac gactggcggg gaacgtgggc ggcggtcggg
     3121 gtctttctgg ccgcggccgt gggcctgcca gcgtgggttg ggcggcggtg gtcgctcatc
     3181 cctcgctatc gcgccgtctt cggggcctgg ctgtgggcgg ctgtgttcgt cctctgcctg
     3241 ctgcccgccc ggctggccgg ccccactgcc gccgaggccg ctgccgggct gcaaatcgcc
     3301 gggctgctgc tctatttggc actggtggcc atcgtccgcc gccgcgctgg cgtccgcttc
     3361 gcgtccggcg gccggggcgt ggccgtggcg ctgttcatgg ccgcgctgct ggcgctgccg
     3421 tgggtggtct ggggcgcgct ggggtcgcc
//
LOCUS       DAOSMQ010000348         3443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27519_length_3443_cov_4.746753, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000348 DAOSMQ010000000
VERSION     DAOSMQ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27519_length_3443_cov_4.746753"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="tsaD"
                     /locus_tag="PK829_11010"
     CDS             complement(<1..714)
                     /gene="tsaD"
                     /locus_tag="PK829_11010"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU41783.1"
                     /translation="MSDDSTRILAIETSCDETAAAVIADGTTMLSNVIASQTELHARY
                     GGVFPEVASRRHVEVIHAVVEEAMREAHLGFSDLDCVAVTRGPGLVGSLLVGVNMAKG
                     LAVARNLPILGVNHIEGHIYSLWLTEEAPHIHFPVLTLVVSGGHTELYVMTDHGRYEL
                     LGAALDDAAGEAFDKVGRLLGLPFPGGPAIDRASDKGSPTAFRFPRAVMEEEHGYDFS
                     FSGLKTAVMRQVSQYHSPAT"
     gene            complement(711..2129)
                     /gene="selA"
                     /locus_tag="PK829_11015"
     CDS             complement(711..2129)
                     /gene="selA"
                     /locus_tag="PK829_11015"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOU41784.1"
                     /translation="MEPAAASSPAVTLRNLPSIDRLLRAPGGDELLAAFGRALATEAL
                     RATLDAARTAVRDGLPALPPDYDLVAAAYEWATDLLAPTLRPVINATGIIVHTNLGRA
                     PLGETALAAVAAAAAGYATLEYDLAAGRRGSRAVHAEQLLTRLTGAEAALVVNNNAAA
                     VLLMLTALCRDAEVIISRGQLVEIGGGFRIPEVMAQSGAQLVEVGATNRTHLRDYAEA
                     ITARTAAILVAHPSNYRVVGFTSQPELAELAALAHERGLWLLFDQGSGALRDTTAYGL
                     ALEPTVPDGLRAGADVVAFSGDKLLGGPQAGLLVGRAELVRRMQRHPLARAVRADKMA
                     LAALAATLGHYLAGQPQRVPVWQMIARPLDDIAAEAERWAARLAALGVPAEVVAGESL
                     VGGGSLPGETLPTRLVALSSGGAEALAARLRAAAVPVVGRIQDGRLLLDPRTVLPGQA
                     EQLLETVARAAAGHAGKEHVHS"
     gene            complement(2129..2632)
                     /locus_tag="PK829_11020"
     CDS             complement(2129..2632)
                     /locus_tag="PK829_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41785.1"
                     /translation="MHRFVRRLGVVPVLALSAGLIVLGVLAVDRIVNTMWPFDVTRIE
                     LVRATAQQRVDAAMLLAAAKSDIIIAFLASVIVAVTGLALPLAYFFNKRIQPPELRHE
                     PPPFLTISRQALWVGLWVAFCLWLQMNRTLGLAVAALGAAVLILFEVVLQIRARASAI
                     QEQGSVT"
     gene            complement(2640..3089)
                     /locus_tag="PK829_11025"
     CDS             complement(2640..3089)
                     /locus_tag="PK829_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HOU41786.1"
                     /translation="MIPMTLAVELKSAGLVWRTSTYDFFAIPDHGLDDRIFVLADMVA
                     FTELVQGWPAVAFHGTAEWALDYIFTDEIVWMPTEEQLRDTLQDNLRRDAGDALSLLL
                     RLGPGEDYHCDIGFRGQSLTFDGPTAGEAYARALLHVLGQRNQPNGV"
     gene            3158..>3443
                     /locus_tag="PK829_11030"
     CDS             3158..>3443
                     /locus_tag="PK829_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU41787.1"
                     /translation="MSALPANFTHLHLHSHYTLLGATPSLDDLVDRARRDGLRQLALA
                     DSNALFGAVSFARACRAAGIRPIIGLTATVALPADDLPPDAHALPGHLVLL"
BASE COUNT          546 a         1199 c         1182 g          516 t
ORIGIN      
        1 cgtcgctggc gagtggtact ggctgacctg gcgcatgacc gccgttttca ggccgctgaa
       61 gctgaagtcg tagccgtgct cctcctccat gacggcccgc gggaagcgaa aggcggttgg
      121 gctgcccttg tccgacgccc ggtcgatggc cgggccgccg gggaagggca ggcccagcag
      181 ccggccgacc ttatcgaagg cctcgccggc ggcgtcatcg agggccgcgc cgaggagttc
      241 atagcggccg tggtcggtca tgacgtacag ttcggtgtgg ccgccgctga cgaccagcgt
      301 cagcaccggg aagtgaatgt gcggcgcttc ctcggttagc cacagcgagt agatgtgccc
      361 ctcgatgtgg ttgacgccca ggatgggcag gttgcgggcc acggccaggc ccttggccat
      421 attgacgccc accagcagcg agccgaccag ccccggcccg cgcgtgacgg ccacgcagtc
      481 aaggtcgctg aagccgagat gggcctcgcg catggcctcc tcgacgacgg cgtggatgac
      541 ctccacgtgg cggcgcgagg ccacctcggg gaagacgccg ccatagcggg cgtgcagttc
      601 ggtttggctg gcgataacgt tgctcagcat ggtcgtgcca tcggcgatga cagccgcggc
      661 cgtctcatcg cacgaggttt cgatggccag gatgcgggtc gaatcgtcgc tcatgagtgt
      721 acatgttcct tgccggcgtg ccccgcggcc gcccgcgcca cggtttccag caactgctcc
      781 gcctggcctg gcaaaacagt gcgcgggtcg agtagcagac ggccgtcctg gatacggccg
      841 acgaccggca cagccgccgc gcgcagccgc gccgccagcg cctcggcccc gccggagctt
      901 aaggccacca gccgcgtcgg cagcgtctcg ccgggcaggc tgccaccgcc gacaagtgac
      961 tcgccggcca cgacctcggc cggcacgccc agcgctgcca gccgcgccgc ccaacgctct
     1021 gcctcggcgg cgatgtcgtc cagcggccgg gcgatcatct gccagacggg cacgcgctgc
     1081 ggctggccgg ccagatagtg gcccagcgtc gccgccagcg cggccagagc catcttatcg
     1141 gcgcgcacgg cccgcgccaa ggggtggcgc tgcatgcgcc gaaccagttc cgcccgcccg
     1201 acgagcagcc cagcctgcgg cccgcccagc aacttatcgc cgctgaaggc caccacgtcc
     1261 gccccggcgc gcagcccgtc gggcacagtc ggctccaggg ccaggccata ggcggtcgtg
     1321 tcgcgcaaag cgccgctgcc ctggtcgaat agcagccaca ggccgcgctc gtgggccagc
     1381 gcggccaact cagccagctc tggttggctg gtgaagccga cgacgcggta gttagagggg
     1441 tgggcgacga gaatcgccgc cgtccgcgcg gtaatcgcct cggcgtagtc gcgcaggtgg
     1501 gtgcggttgg tcgcgccgac ttccaccagt tgcgcccccg attgggccat gacctccggg
     1561 atgcggaagc cgccgccgat ctccaccaac tggccgcggc tgatgatgac ctcggcgtcg
     1621 cggcagagcg cggtcagcat gagcaggaca gcggcggcgt tgttattgac taccagagcc
     1681 gcctccgcgc ccgtcagccg ggtcagcagc tgctcggcgt ggacggcccg cgaaccgcgc
     1741 cgtcctgcgg ccaggtcgta ctccagcgtg gcatagcccg ccgcggccgc ggccacggcc
     1801 gccagcgccg tctcgcccaa cggtgcgcgc cccaggttgg tgtggacaat gatgcccgtg
     1861 gcattgatga ctggccgcag cgtcggcgcg agcaagtcgg tggcccattc gtaggccgcg
     1921 gcgacgaggt cataatcggg cggcaacgcc ggtaggccgt cgcggacggc cgtgcgcgcc
     1981 gcgtccagcg tcgcccgcag cgcctcggtg gccagggcgc gaccgaaagc ggccagcaat
     2041 tcgtcgccgc cgggcgcgcg cagcagccgg tcgatgctgg gcagatttcg cagcgtgacg
     2101 gcaggagagg acgcggcggc cggctccatt acgtgacgct gccctgttcc tggatggccg
     2161 aggcgcgggc acggatttgc agcacaacct cgaagaggat tagcacggcc gcccctagcg
     2221 cggccacggc cagacccagc gtgcggttca tctggagcca caagcagaag gccacccaca
     2281 ggccgaccca cagcgcctgg cggctgattg tcagaaaggg cggcggttcg tggcgcaact
     2341 ccggcggctg gatgcgcttg ttgaagaagt aggccagcgg cagcgccaac ccggtcacgg
     2401 cgacgatgac cgaggccaaa aaggcgatga tgatgtcgct cttggccgcc gccagcagca
     2461 tggccgcgtc aacgcgctgt tgcgccgtgg cccgcaccaa ctcgatccgg gtcacgtcaa
     2521 acggccacat cgtattgacg atgcgatcta cggccagcac gccgagcacg atcagaccgg
     2581 cgctgagcgc caggacaggc acaaccccca ggcggcggac aaatctgtgc atgggcggat
     2641 tatacaccat ttggttgatt acgttgcccc agcacgtgga gcagggcgcg ggcgtaggcc
     2701 tcgccggccg tgggcccgtc gaaggtcagc gactggcctc gaaagccgat gtcgcaatgg
     2761 tagtcctcgc ccggccccag gcgcagcagc agcgacaggg cgtcgccggc gtcgcggcga
     2821 agattgtctt gcagggtgtc gcgcaactgt tcctcggtcg gcatccagac gatctcgtcg
     2881 gtgaagatgt agtccagtgc ccactcggcc gtgccgtgga aggccactgc cggccagccc
     2941 tgtaccaact ccgtgaacgc gaccatatcg gccaggacga aaatgcggtc gtccaggccg
     3001 tggtcgggga tggcgaagaa gtcataggta ctcgttcgcc agacgaggcc agcactcttt
     3061 agttcaaccg ccagggtcat cgggatcatc gctgttctcc ataggggccg attgtcgctt
     3121 gccagtcggc ggactcactt ctatcatcgc ggctgtgatg agcgcgttgc cggccaattt
     3181 tacccacttg cacctgcatt cgcattacac gttgctgggg gcgacgccat cgctcgatga
     3241 tctcgttgac cgtgcccggc gcgacggcct gcgacagttg gccctggccg acagcaacgc
     3301 ccttttcggc gcggtcagct ttgcccgcgc ctgccgcgcc gccggcatcc ggcccatcat
     3361 cggcctgacg gccaccgtgg ccttgccggc tgacgatcta ccgcccgacg cccacgccct
     3421 gcccggccac ctggtgctgc tgg
//
LOCUS       DAOSMQ010000349         3434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27603_length_3434_cov_4.234685, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000349 DAOSMQ010000000
VERSION     DAOSMQ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27603_length_3434_cov_4.234685"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2537)
                     /locus_tag="PK829_11035"
     CDS             complement(<1..2537)
                     /locus_tag="PK829_11035"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HOU41788.1"
                     /translation="MSAENKARGRGGAGEQGSESDALRRWRPATDHWPLLAILATFLV
                     LGLATSAINPLHEATDELRHYRFIQHIIQRHALPVQGQTSDLPCTIQGHHPPLYYATA
                     ALLTFWIDTGRDICYEPPTNPFWDYRYWEVGRDNKNQYLHGADESFPWHGEALAAHLA
                     RVVNLLYGAATVWLTWATARLIWPRRPALAAGSAAFVAFNPMFLYMAAAINNDVAAAM
                     AGAAVTYACVRLLRDPRGLRPAWGVALGALYALALMSKFNLAAIIIVIEAAVTWVAWR
                     RGQWRQWIVVNVLLVGVTALLAGWWFLRNQRLYGEPTGIEELTQLWGVRDPRQSFWLA
                     VSELPYAWTSLWGRFGYGQIPLPGWMYGALFVVLLLAVGGYGVALLRRLPRGDRTPSP
                     SGRGLGRGSDADAPSPNPSLREGEVLALLALTIAVFFAVLFNYLLVSPAGPMGRFFFP
                     ALPALGILMFYGLTLWGKGPGARGQGPGEEAHVKARRGQVAKAEKKLAFLPWRLGFFA
                     PLRERSPLKSGTWSLEPFSLEPILAWAVNLGLIALSLVALFGYLRPAYARPAAWAEGA
                     TLPNPVAIQFDTLVRLRGYAIDTTAVRPGEPIGIDLYWEVTGQPPGDYLLFVHLVDSE
                     TGALVAQRDTHPGLGNFPSSQWRPGDRFVERLRLYVPETAYVPAAAEVRVGLYAPGSY
                     RLGITDAATGAGLGDSFPLGAVAIVLAGPDTPLPNAGVYRFEDRFRLAGYAYDRRVLA
                     AGEPLTVTLYWTAGPAPAERYDVQVRLLDEAGNIVHHEQRPLPVLPPGETVADVHVIP
                     GDPARTPGNYTVQVSIEGADDRRLQLVGEDGRWLDDRLLLSAVRVTD"
     gene            complement(2537..>3434)
                     /locus_tag="PK829_11040"
     CDS             complement(2537..>3434)
                     /locus_tag="PK829_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41789.1"
                     /translation="GALAGWARLAAARRGWAAGLLAALLLVATAARDLPAYFGEWPAR
                     GMVRYLYRADIEDVADFVLRDPAAPWPADFGITGLLAGPWDRVALELALGGRDDVRPR
                     WYDPRRALLLWPDISFSGYPGVESPYAGLFAPLPGDNLLRGDYTLARVAPSVTPALDG
                     QGFLSEAPLCFANGLCWMAAVYDAAGGQLELEWRVESPLALPPLPLISNPPPPGVYSG
                     PRLYVFAQLVDDSGTFLVGDDGLWVDPQTLQPGDVFLQRHLLPAPAGARPAAVLFGLY
                     DPMTGERIPTVEGQDHVRLEIE"
BASE COUNT          577 a         1237 c         1186 g          434 t
ORIGIN      
        1 taatccgtca cgcgcacggc cgacagtaac agcctgtcat ccagccagcg cccatcctcg
       61 cccaccagtt gcagccgccg gtcgtccgcg ccctcgatgg acacctggac tgtgtagttg
      121 cccggcgtcc gggccgggtc accggggatg acgtgaacat cggcgaccgt ctcgccgggc
      181 ggcaggacgg gcagcggccg ctgctcgtgg tggacgatgt tgccggcctc gtccagcagc
      241 cgcacctgca cgtcatacct ctccgccggg gccgggccgg ccgtccagta gagcgtcacc
      301 gtcagcggct cgccagcggc cagcacgcgc cggtcgtagg catagccggc cagccggaag
      361 cgatcctcga agcggtagac gccggcgttg ggcagcggcg tgtccgggcc ggccagcaca
      421 atggccaccg cgcccagcgg gaagctatcg cccaggcccg cgcccgtggc cgcgtcggtg
      481 atgcccagcc ggtagctgcc gggggcgtag aggccaacgc gcacctcggc cgccgcgggc
      541 acataggctg tctccggcac atagagccgc agccgctcca cgaagcggtc gcccggtcgc
      601 cattggctac tggggaagtt gcccaggccg gggtgggtgt cgcgctgggc caccagcgcg
      661 cccgtctcgc tatcgaccag gtggacgaac agcaggtagt cgcccggcgg ctggccagtg
      721 acttcccagt agagatcgat gccgatcggc tcgccggggc ggacggcggt cgtatcaatg
      781 gcgtagccgc gcaggcgcac cagcgtatcg aactggatgg cgaccgggtt gggcagcgtc
      841 gcgccctcgg cccaggccgc cggccgcgcg taggccggcc gcaagtagcc gaacagggcc
      901 accagcgaca gggcgatcag gcccaggttg acagcccagg cgaggatggg ttccagggaa
      961 aaaggttcca ggctccaggt tccagatttc aggggagagc gctcacgcaa aggcgcaaag
     1021 aagccaaggc gccaaggaag aaaggcaagc ttcttctctg ccttcgcgac ttggcccctt
     1081 cgcgccttga cgtgagcctc ttcccctggc ccctggcccc tggcccctgg ccccttcccc
     1141 cacagtgtca gcccatagaa catcaaaatc cccaacgccg gcaacgcggg gaagaagaag
     1201 cgccccatcg gcccggccgg gctgacgagc aggtagttga agaggacggc aaagaagacg
     1261 gcgatggtca gcgccagcag cgccagcacc tccccctccc tcagggaggg gttaggggag
     1321 ggcgcgtccg cgtcagaccc tctccccaac cctctcccag agggcgaggg ggtccgatcc
     1381 ccccgaggga gccggcgcag gagagcgacg ccatagccac ccacggccag cagcaatacc
     1441 acgaacaacg cgccatacat ccagcccggc agcgggattt gcccgtagcc gaagcgcccc
     1501 cacaggcttg tccaggcgta gggcagctcc gacacggcca gccagaagct ctggcgtggg
     1561 tcgcgcacac cccatagctg tgtcagttcc tcgatgcccg tcggctcgcc gtagagccgc
     1621 tgattgcgca ggaaccacca accggccagc agcgccgtca cgccaacgag caggacgttg
     1681 acgacgatcc actgccgcca ctgcccccgc cgccaggcca cccaggtaac ggccgcctca
     1741 atgacgataa tgatggccgc caggttgaac ttgctcatca gcgccagcgc gtagagcgcc
     1801 cccagggcca cgccccacgc cggccgcagc ccacgcgggt cgcgcagcag ccgcacgcag
     1861 gcgtaggtca ccgccgcgcc agccatcgcc gcggcgacgt cgttgttgat ggccgcggcc
     1921 atgtagagga acatcgggtt gaaggccacg aaggcggccg acccggccgc cagcgccggc
     1981 cgccgtggcc agatgagtcg cgccgtggcc caggttagcc agaccgtggc cgcgccgtag
     2041 agcaggttga ccacccgcgc caggtgcgcc gccagcgcct cgccatgcca gggaaagctc
     2101 tcgtccgcgc cgtggaggta ttggttcttg ttgtcgcgcc ccacttccca gtagcggtaa
     2161 tcccagaagg gattggtcgg cggctcgtag cagatgtcgc ggccggtgtc gatccagaag
     2221 gtcagcagcg ccgccgtggc gtagtagagc ggcgggtggt ggccctgaat ggtgcagggc
     2281 aggtcgctcg tctgcccctg caccggcagc gcatgccgct ggatgatgtg ttggatgaag
     2341 cggtaatggc gcagctcgtc ggtggcctcg tgcagcgggt tgatggcact ggtggccagg
     2401 ccgaggacga ggaaggtggc caggatagca agcagtggcc agtggtcagt ggccggtcgc
     2461 caacggcgga gggcatcgct ttcgctcccc tgctcccctg ctcccccgcg cccccgcgcc
     2521 ttgttctccg cactcatcac tcgatctcca gccgcacgtg gtcttgcccc tcgaccgtgg
     2581 ggatgcgctc gccggtcatg gggtcgtaga ggccaaagag gacggccgcc ggacgcgccc
     2641 ccgccggcgc gggcagcaag tggcgttgca ggaagacgtc gcccggttgc agcgtctgcg
     2701 gatcgaccca caggccatcg tcgccaacaa ggaacgtgcc gctgtcatcg acgagttggg
     2761 cgaagacata caggcgcggg ccggagtaga cgcccggcgg cggcgggttg ctgatcagcg
     2821 gcagcggcgg cagcgccagc ggcgactcga cgcgccactc caattccagt tgcccgcccg
     2881 cggcgtcata gacggccgcc atccagcaca gcccgttggc gaagcacagc ggcgcttcgg
     2941 acaggaagcc ctgcccgtcg agcgccggcg tcaccgaggg ggcgacgcgg gccagggtgt
     3001 agtcgccacg cagaaggttg tcgcccggca gcggcgcgaa cagccccgcg tagggcgact
     3061 ccacgccggg gtagccgctg aagctgatgt cgggccagag cagcagggca cgccgcgggt
     3121 cataccagcg cggccgcacg tcatcgcgcc cgcccagagc caactccagg gcgacacgat
     3181 cccacggccc ggccagcagc ccggtgatgc cgaagtcggc cggccagggc gcggccgggt
     3241 cgcgcagcac gaagtcggcc acgtcctcga tgtcggcccg gtagaggtag cgcaccatgc
     3301 cgcgcgccgg ccactcgccg aagtaggccg gcaggtcgcg cgccgccgtg gccacgagca
     3361 gcagcgccgc cagcagcccc gccgcccacc cgcgccgcgc cgcggccagc cgcgcccagc
     3421 cggccagcgc cccc
//
LOCUS       DAOSMQ010000350         3408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27875_length_3408_cov_4.235908, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000350 DAOSMQ010000000
VERSION     DAOSMQ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27875_length_3408_cov_4.235908"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1376
                     /locus_tag="PK829_11045"
     CDS             <1..>1376
                     /locus_tag="PK829_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOU41790.1"
                     /translation="SNSLRLLRAAAGLAWDELGIAQMYLFGGYTATERTILRGAQRAA
                     AGTEYRFRVGQEGPPAVARLARTWTTVIDDRLDHVVETICDLWTTAAARHIDPVDTPI
                     GIMLSGGLDSRLVAGAVASRGKRIIGLTFGDANSDEVRIAGEVAAAVGAQWLPRPMDE
                     SFAFERLAFDRVNFTNETMYNLMWDANLPELVAAGVTHFSSGATFETTFGGHRDVDPR
                     RRLLKNVRQALIGPWPTPTATAEQLEALIDLHTGKARKRARNYARLLAEPYRHLMTDS
                     LPAIRDEVAARIHAIAASGPIAIEQVNERFDSEQYQGHHSRDQERQLALYGTPLLPTC
                     DRDVANYLTNLPAGMKYDHALYYRVFRRLYPRLAAIQVPNLGTDLNKSQLQIELIRAW
                     RIQRKSRLSSWVNFDAWMRLGNNMAKYERLFLDQAGFFDPAAVGDYFADVRAGRQRLY
                     DGGETGSFL"
     assembly_gap    1377..1476
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1604..>3408)
                     /locus_tag="PK829_11050"
     CDS             complement(1604..>3408)
                     /locus_tag="PK829_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HOU41791.1"
                     /translation="ALVPLTHADEMAVADPATMLADLRAIPEYVQRFQDAFGVSGDAI
                     TTDHVTQALAAFQRTLISDDSPFDRYAAGDFDALSPAQRRGLAIFRSGATRCFECHGA
                     PTFASDTFRVVGVDSDDPGRAGVVEDGVFGAFKVPTLRNVALSAPYMHDGSLATLEEV
                     IDFYRDGGGRAHGFENVDVFVNPFTLTDQERADLLAFLYALTDESGLPEIPAAVPSGL
                     PVVAPVENPARAVVAAMNTGGAGGAAASAARPRTITVAAGETIQAAVDRARPGDTVEI
                     PYGVYHERVAIDMSGITLRGVANAAGEWPILDGQGTLSEGVIASGNDFTVGNLHVRNY
                     TDNGVLVEGARGVHFHDIVAENVGTYGVYPVRSTDVLIERVTVSGVDDAGIYAGQSEN
                     VIVRDSTAYGNVIGIELENTLNGEVTNNHVYDNSNGILIVLLPQLTSKISSGAKITGN
                     LVEHNNTANFAPEGATARIVPPGSGILLIGTDNNEVSGNTITGNKTAGLAIFSLTGTG
                     AFNENELDVGPLAEGNYAHGNTYRNNATDPDPVVAELGIPAGDILWDTTGGNNRFNEP
                     GATSFPPLLPGDGWPGFLRTAYGNIFRVLTGLLG"
BASE COUNT          493 a         1141 c         1154 g          520 t
ORIGIN      
        1 agcaactcgc tgcggctgct gcgggccgcc gccgggctgg catgggacga actgggtatc
       61 gcccagatgt atctcttcgg cggctacacg gccaccgagc gcaccatcct gcgcggggcg
      121 caacgcgccg ccgcgggcac ggagtaccgc ttccgcgtcg gccaggaggg gccgccggcg
      181 gtcgcccgcc tggcccgcac ctggacgacg gtcatcgacg accgcctcga ccacgtcgtc
      241 gagaccatct gcgacctgtg gacgacggcc gccgcccgcc atatcgatcc ggtcgatacg
      301 cccatcggca ttatgctcag cggcgggctg gattcgcgcc tggtggccgg ggcggtggcc
      361 tcgcgcggca agcgcatcat cggcctgacc tttggcgacg cgaacagcga cgaagtgcgc
      421 atcgccgggg aggtggccgc ggccgtgggg gcgcaatggc tgccgcggcc gatggacgag
      481 agcttcgctt tcgagcggtt ggccttcgac cgggtgaact tcaccaatga gacgatgtat
      541 aacctgatgt gggacgccaa tctgccggag ttggtggccg cgggcgtgac ccacttcagc
      601 agcggagcga ccttcgagac gaccttcggc ggccaccgcg atgtcgatcc gcgccgccgt
      661 ctgctgaaga acgtccgcca ggcgctgatc gggccgtggc ccacgcccac ggccacggcc
      721 gagcaactgg aggcgctcat cgacctccac accggcaagg cccgcaagcg cgcccgcaac
      781 tacgcccgcc tgctggccga gccataccgc cacctcatga ccgacagcct gcccgctatc
      841 cgtgatgagg tcgccgcgcg catccacgcc atcgccgcct ccggccccat cgccatcgag
      901 caggtcaacg agcgcttcga cagcgagcag tatcagggcc accacagccg cgaccaggag
      961 cgccagctgg ccctttatgg cacgccgctg ctaccgacct gcgaccggga cgtggccaac
     1021 tacctgacca acctgccggc gggcatgaag tacgaccacg ccctctacta ccgcgtcttc
     1081 cgccgcctct acccccgtct ggccgccatc caggtgccca acctgggcac cgatctgaac
     1141 aagtcgcagt tgcagatcga actcatccgc gcctggcgca tccagcgcaa gtcgcggcta
     1201 agctcgtggg tcaacttcga tgcctggatg cgcctgggca acaacatggc taaatacgag
     1261 cggctgttcc tcgaccaggc gggcttcttc gacccggccg ccgtgggcga ctacttcgcc
     1321 gacgtgcgcg ccggccgcca gcgcctctac gacggcggcg agacgggcag cttcctnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgt gcgcgccggc cgccagcgcc
     1501 tctacgacgg cggcgagacg ggcagcttcc tgaatctggc ctatctgctg gacgagcggc
     1561 gggcggcggt gcggccgtcg ccccacccca ccaccgccac ggcctagccc agcagcccgg
     1621 tcagcacgcg gaagatgttg ccataggccg tccgcaggaa gccgggccag ccgtcgccgg
     1681 gcagcagcgg cgggaagctc gtcgcccccg gctcgttgaa gcggttgtta ccgccggtcg
     1741 tgtcccagag gatgtcgccc gcggggatgc ccaactcggc cacgaccggg tcggggtcgg
     1801 tggcattgtt gcggtaggta ttgccgtggg cgtagttgcc ctcggccagt ggccccacgt
     1861 ccagctcgtt ctcgttgaat gcccccgtgc ctgtcaggct gaagatggcc agcccggccg
     1921 tcttgttgcc ggtgatagtg ttgccgctga cctcgttgtt gtccgtgccg atgagcagga
     1981 tgccgctgcc gggcggcaca atgcgcgccg tggccccctc cggcgcgaag ttggccgtgt
     2041 tgttgtgctc caccaggttg ccggtgatct tcgccccgga ggagatcttc gacgtcagtt
     2101 gcggcagcag gacgatgagg atgccgttgc tgttgtcgta aacgtggttg ttggtcacct
     2161 cgccgttgag ggtgttctcc aattcgatgc caatgacgtt gccataggcc gtgctgtcgc
     2221 ggacgatgac gttctcgctc tgcccggcgt agatgccggc gtcgtccacg ccgctgacgg
     2281 tgacccgctc gatgagcacg tcggtgctgc gcacggggta gacgccatag gtgccgacgt
     2341 tctcggcgac gatgtcgtgg aagtggacgc cgcgcgcgcc ctcgaccagc acgccgttgt
     2401 cggtgtagtt gcgcacgtgg aggttgccga cggtgaagtc gttgccggag gcgatgacgc
     2461 cctcggacag cgtgccctgg ccatcgagaa tgggccactc gccggcggcg ttggccacgc
     2521 cgcgcagggt gatgccgctc atgtcgatgg ccacgcgctc gtggtagacg ccgtagggaa
     2581 tctcgacggt gtcacccggt cgcgcccggt caacggcggc ctggatggtc tcgccagcgg
     2641 cgacggtgat cgtccgcggc cgggccgcgg acgcggcggc cccgcccgcc ccgccggtgt
     2701 tcatcgccgc gacgacggcc cgcgcggggt tctccaccgg ggccacgacc ggcagcccgg
     2761 agggcacggc cgcgggaatc tccggcaagc cgctctcatc ggtcagagcg tagaggaagg
     2821 ccagcaggtc ggcccgctcc tggtcagtca gggtgaacgg gttgacgaag acatcgacgt
     2881 tctcgaagcc gtgagcgcgc ccgccgccgt cgcggtagaa gtcgatgacc tcttccaggg
     2941 tggccagcga gccgtcgtgc atgtaggggg cgctgagggc gacgttgcgc agcgtcggca
     3001 ccttgaacgc gccgaagacg ccatcttcga ccacgccggc gcggccgggg tcgtcactgt
     3061 caacgcccac gacgcggaag gtgtcgctgg cgaacgtcgg cgcgccgtgg cactcgaagc
     3121 agcgcgtcgc cccggagcgg aagatggcca gcccgcgccg ctgggccggc gagagggcgt
     3181 cgaagtcgcc ggcggcatag cggtcgaaag ggctgtcgtc gctgatgagc gtgcgttgga
     3241 aggcggccag agcctgcgtt acgtggtcgg tcgtgatcgc gtcgccgctc acgccgaatg
     3301 cgtcctggaa gcgctggacg tattcgggaa tggcccgcag gtcggccagc atcgtcgccg
     3361 ggtcggccac ggccatctcg tcggcgtggg tcaggggcac gagggcct
//
LOCUS       DAOSMQ010000351         3405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27907_length_3405_cov_5.074328, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000351 DAOSMQ010000000
VERSION     DAOSMQ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27907_length_3405_cov_5.074328"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PK829_11055"
     CDS             <1..910
                     /locus_tag="PK829_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41792.1"
                     /translation="GYAAPEQYGKGQTDARSDVYALGATLHFLLTGRDPADDPFNFPP
                     VRALNPAVSPALEAAVAQALRGAPDTRWQSAREMRRALTQTVATPLAVESKPMSGAVS
                     SRRASLAPTPAATARPSGRAQPRPAAAGVSRPVARPAAARSPTPRPAAPHWRDSGWFY
                     LFVVGGLAAVTIFVQELGFLGPLFPLVWFTLSMLAGTLARQTGAIGFTWLSVPLYNSI
                     VGQTNDLSWAMVLSLIMPIELFFLLTRYRLYTPAAMLLAGGLGVMGMYVITNPAVGDL
                     VLSGAAVALGSLVAYVIDQWLEPLLT"
     gene            958..2022
                     /locus_tag="PK829_11060"
     CDS             958..2022
                     /locus_tag="PK829_11060"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOU41793.1"
                     /translation="MNEQGRPCPNCGTIARPGAHFCSRCGADLTAGTTPQLASLPVAP
                     ALPPGDDAPTQVHLDTPPGTAEAPTVAYPPPADAVTLTGMQAIQQRALRQNSGTATHA
                     GKVRSHNEDYVAKLDYSLDQSGAAEPMGLYIVADGMGGYKEGEKASRDSVRQAFRDFI
                     EGHILQDLQSTTHKLSTGQPDATVEETLRALVTEANRLVYRKRQQTGTRRGTTITAVL
                     IVGAKAAVANVGDSRTYLMRGGQLHQVTQDHSLVYSLFKAGQITEEQIYSHPKKNEVY
                     RSLGEQEDIKVDIFPLTIQAGDRFLLCSDGLWEMVRNPQIAAILGNAVTPQAACDHLV
                     AAAFDNGGEDNISALAVYLE"
     gene            2205..>3405
                     /locus_tag="PK829_11065"
     CDS             2205..>3405
                     /locus_tag="PK829_11065"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU41794.1"
                     /translation="MANTMEFSGLFNKSFLPYMAQPQLLYAYFEARPGGGTRGASTAR
                     LPLNFSLVLDRSGSMSGPKITQLREAVRTLIGRLEPTDTVAVVAFNAHSDVLIPATNA
                     GNRAVLSERIGRLEAGGGTKMAPAMRAGLAEIAKARPSHLSSAVASRLIVLTDGQTTG
                     EDDCRAEADRVAAAGVPLIALGLGSDWNEDLLLDLARRTGALGDAFLMKGPEDVGPIF
                     DQVFTQMKVVAQDVAFRLLLVQGVEARRVWQVTPLIKDITFGAVRGRTVTFGLPELAE
                     SGAAFLVELLIPPRNPGQYRFAQAQATYHLPGQPAEEARVDLTAEVTTDPNLARGVNG
                     RVMNIVEKVTAFKLQTQALDDAARQDIANATRKLRAAHTMLLEQGEVELAQNALAEAE
                     RLESGQGL"
BASE COUNT          558 a         1158 c         1132 g          557 t
ORIGIN      
        1 gggctacgcc gcgccggagc aatatggcaa ggggcagacc gacgcccgca gcgatgtcta
       61 tgccctgggg gcaacgctgc acttcctgct gaccggtcgc gacccggctg acgacccgtt
      121 caacttcccg ccggtgcgcg ccctgaatcc ggccgtgtcg ccggcgctgg aggcagccgt
      181 ggcccaggcg ctgcgcggcg cgccggacac gcgctggcag tcggcgcggg agatgcggcg
      241 ggcgctgacc cagaccgtgg ccacgccgct ggccgtcgag tcgaagccga tgtctggggc
      301 tgtgtcgtcg cgccgcgcgt ccctggcccc aaccccggct gcgacggcgc ggccgtccgg
      361 ccgcgcccaa ccgcgcccgg cggccgcggg ggtgtcgcgt cccgtcgcgc ggccggcggc
      421 ggcgcgctcc cccacgccgc gcccggccgc gccccattgg cgtgacagcg gctggttcta
      481 cctgttcgtt gtcggcgggc tggccgcagt gactatcttt gtgcaggagc ttggttttct
      541 ggggccgctc tttccgctgg tctggttcac attgagcatg ttggcgggca cgctggcgcg
      601 gcaaactggg gcaattggct ttacgtggtt aagcgttccg ctttacaata gtatcgtcgg
      661 gcagaccaat gacttgagtt gggcgatggt attgtcgctc attatgccca tcgagttgtt
      721 ctttctgttg acacgctacc gcctctatac gcccgcggct atgctcttgg ccgggggcct
      781 gggggtgatg ggaatgtacg tgatcaccaa tccggcggtg ggtgatctgg tgttgagcgg
      841 cgcggcggtc gccctcggca gccttgtcgc ctatgtgatc gatcaatggt tggaaccgct
      901 gctcacgtag ccacgcgccg tcggacggcg acgcggccgg ttaggagaga tgcgccgatg
      961 aacgaacaag gccggccttg cccaaattgc ggcaccatcg cgcgccccgg cgcgcacttc
     1021 tgttctcgtt gcggcgctga cctgacggcg ggcacaacgc cgcaattggc cagcttgccc
     1081 gttgcgccgg cgttgccgcc cggcgatgac gcgccgaccc aggttcacct cgacacacct
     1141 cccggcacgg cagaagcgcc gaccgtcgcc tatccgcctc cggccgacgc cgtgacctta
     1201 accggtatgc aggccatcca acagcgcgcc ctgcgacaga actccggcac ggccacgcac
     1261 gccggcaagg tgcgcagcca caacgaggac tacgttgcca agctcgacta cagtctcgac
     1321 caaagcggcg cggccgagcc aatgggcctc tacatcgtcg ccgacggcat gggcggctac
     1381 aaggagggcg agaaggccag ccgcgactcg gtgcggcagg cgtttcgcga cttcatcgag
     1441 ggccacatcc tgcaagactt gcagtcaacg acacacaagc tctcgaccgg ccagcccgac
     1501 gcgaccgttg aggagacgtt gcgcgcgttg gtgaccgagg ccaaccggct ggtctatcgc
     1561 aagcggcaac agaccggcac tcggcgcggc acgactatca ccgccgtgct gatcgtcggg
     1621 gccaaggcgg ccgtggccaa cgtcggcgac agccgcacgt acctgatgcg cggcggccag
     1681 ctacaccagg tgacacagga tcactccctc gtctatagcc ttttcaaggc cggccagatc
     1741 accgaggagc agatctacag ccaccccaag aaaaacgagg tctatcgttc gctgggcgag
     1801 caggaagaca ttaaggtcga tatcttcccc ctgactattc aggctggcga ccggtttttg
     1861 ctctgctccg atggtctgtg ggagatggtt cgtaacccac aaattgctgc catcctgggc
     1921 aacgcggtca cgccgcaagc ggcctgcgac cacctggtgg ccgccgcctt cgataatggt
     1981 ggcgaggaca acatcagcgc cctggcggtt tacctggagt agcgccgccc gtggcgcgat
     2041 tctccgaatc gcaagcgtgg cgcgattctc cgaatcgcaa gcgtggcgcg attctccgaa
     2101 tcgcaagcgt ggcgcgattc tccgaatcgc aagcatgatc gcaagcatgg cgcgattctc
     2161 cgaatcgcgt tccgcgccgc ccgtccgcgg aaggagacaa ggcgatggcc aacacgatgg
     2221 aattcagcgg cctgttcaac aaaagcttcc tgccctatat ggcccagccg cagttgctct
     2281 acgcttactt tgaggcgcgg ccgggcggcg gcacgcgcgg cgcgtccacg gcgcgcctgc
     2341 cactcaactt cagcctcgtg ctcgaccgca gcggctcgat gagcggcccg aagattacgc
     2401 agttgcgcga ggcggtgcgg acgctcatcg gccggctgga acccaccgac accgtcgccg
     2461 ttgtcgcctt caacgcccat agtgacgtgc tcattccggc tacgaacgcc ggcaaccggg
     2521 ctgtcctgtc cgagcgcatc ggccggttgg aggccggcgg cggcacaaag atggccccgg
     2581 ccatgcgcgc cggcctggcc gagatcgcca aggcccggcc gtcccacctc tcctcggccg
     2641 ttgccagccg cctgatcgtc ctgaccgacg gccagacgac gggcgaagac gactgccgcg
     2701 ccgaggccga ccgtgtggcc gcggccggcg tgcccctgat tgccctgggg ttgggttcag
     2761 actggaatga ggacttgctg ctcgacctgg cccggcggac gggcgcgctg ggcgatgcct
     2821 tcctgatgaa ggggccggag gacgtcggcc ccatcttcga ccaggttttc acgcagatga
     2881 aagttgtggc ccaggacgtg gctttccggc tactgctggt gcagggcgtc gaggcgcggc
     2941 gcgtctggca ggtgacgccg ctcatcaagg acatcacctt tggcgcggtc agaggccgga
     3001 cggtcacctt cgggttgccg gaactggccg agagcggcgc ggccttcctg gtcgagctgc
     3061 ttatcccgcc gcgtaacccc ggccagtacc gcttcgccca ggcacaggcg acctatcacc
     3121 tgcccggcca gccggccgag gaggcgcgcg tcgatttgac cgccgaggtg acgactgacc
     3181 cgaacctcgc ccgcggggtg aacggccggg tcatgaacat cgtcgaaaag gtgactgcct
     3241 tcaagctgca aacgcaggcc ctcgacgacg ccgcgcggca agatatcgcc aacgccacgc
     3301 gcaagctgcg cgccgcccac accatgctgc tggagcaggg cgaggtggag ttggcccaga
     3361 acgcgctggc cgaggccgaa cgcctggaaa gcggccaggg cctgt
//
LOCUS       DAOSMQ010000352         3399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_27979_length_3399_cov_4.002990, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000352 DAOSMQ010000000
VERSION     DAOSMQ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27979_length_3399_cov_4.002990"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..722
                     /locus_tag="PK829_11070"
     CDS             123..722
                     /locus_tag="PK829_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOU41795.1"
                     /translation="MFPKEHKRTGPGLAALGVGLLLLVVVLALGGGRAAASPSATIVR
                     CDPATVVGPIGGAVNVDIYVENVVELYAADVRLSFDTTALQVVDADPEASGVQMQLLD
                     TFLSPDYVPRNVADNDEGTIWYAATQMNPAEAVSGSGPLARTTFRALRPGAFTLPITY
                     YKLVRRNSLEIPATAQGCAVTFVEIDSGGVTFVPFTAKN"
     gene            complement(719..>1453)
                     /locus_tag="PK829_11075"
     CDS             complement(719..>1453)
                     /locus_tag="PK829_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41796.1"
                     /translation="IPPAQCALWPLLAQTHLARGDAGAASAALAARSPDCPADAAARE
                     AQTAVLRAELAAAQAAGDAAAADRAAAALLALDPGDAGALAVLTVVDVLAWLGDGRLD
                     VRAGGSPEPVEARRFVMPDSGDAGDVVFAHPPAAVAFTTALPDAPAVLRFRAALDPQS
                     WGWGGDGVTFVVTAQTAGAPAQELARFHLANDVEGRGWHAVELPLDAFAGQTVTITLA
                     TEAGPAGDGTGDWAGWGAPRVVRRID"
     assembly_gap    1454..1553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1554..2919)
                     /locus_tag="PK829_11080"
     CDS             complement(<1554..2919)
                     /locus_tag="PK829_11080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU41797.1"
                     /translation="MATRDRAPTALPIATAITLLAALLRFLYLGHDSLWFDEVLTRGT
                     AVASLASPAAALAVRDHVPLLYWLTALVLRVLPEHEVTLRLVSALAGVLAVPLLLAFG
                     RAARLPAAGRWAALLLAVSPFHIRYSQEARHYALLLFFSLLVLYWLYRATAAGARGDG
                     RAGRTWLAFAAATAANLLTHYSAWLWLAGQGAAVALWLAWRARQGDRRAALAALPAAL
                     IIGVTLLLLLGRAADALRANTGGAAGTTAAAALPVWLRALWLEFGFFRPWPAAALALL
                     AAVGAVALRRRPGVAALLAVCAVVPVALIQALAITRFALPKYVIYLLPIYLFAAGAGI
                     DAALALVRRPRARAAAGAAVALAILLVAAPAVAAEYRQMVHDWRGAAAQLGPAAPGDV
                     VLTIALDTGDGFNAAGVVAPIYLDPGFRLLDGNHLTADELAALAGARGRVSALALNLY
                     APVVA"
     gene            complement(3015..>3399)
                     /locus_tag="PK829_11085"
     CDS             complement(3015..>3399)
                     /locus_tag="PK829_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU41798.1"
                     /translation="IAEARPSTRAALRALLEHDPLCERVTEATDAQAALAALSENPDL
                     LLLDWALPGLPAAAVVARVRSVRPELVIIALGHHAATRRAALEAGADCFIDTNQPPTD
                     LVALLNTLCSPAAARLGQIGHGYTG"
BASE COUNT          506 a         1224 c         1191 g          378 t
ORIGIN      
        1 ccgccacaac cagccgcgcg gccctgcttg cggtggtcgc cgtcctcgtt gtactggtga
       61 tcggcgcgtt gctatggcag cgcggccgat cgagatgaga gtagataagg agaaatgaac
      121 ctatgttccc gaaagagcac aaacgcaccg ggcctggtct ggccgcgctg ggcgtcggcc
      181 tgctgttgct ggtcgtcgtc ctggcgctcg gcggggggcg cgccgccgct tccccgtcgg
      241 ccaccatcgt gcgctgcgac ccggccaccg tcgtcggccc aataggcggc gcggtgaacg
      301 ttgacatcta cgtcgagaac gtcgtcgagc tgtatgcggc cgacgtgcgc ctgagcttcg
      361 acacgacggc cctgcaagtc gtggacgccg accccgaggc tagtggcgtg cagatgcagt
      421 tgctcgatac ctttctgtcg cccgattacg tcccccgcaa cgtggccgac aacgacgaag
      481 gcacgatctg gtatgccgct acccagatga accctgccga agccgtcagc ggctccggcc
      541 ccctggcgcg caccaccttc agagcgctcc ggccgggcgc gttcaccctg cccatcacct
      601 actacaagct ggtccgccgc aactcgctgg agattccggc cacggcccag ggctgcgccg
      661 tcacgttcgt tgagatcgat tcgggcgggg tcaccttcgt gcccttcacg gccaagaact
      721 agtcgattcg tcgcaccacc cgcggcgcgc cccaccccgc ccaatcgccc gtgccgtccc
      781 ccgccgggcc ggcctcggtg gccagggtga tcgtcaccgt ctgcccggcg aaggcatcga
      841 ggggcagctc gaccgcgtgc cagccgcgcc cctcgacgtc gttggccaga tgaaagcggg
      901 ccaactcctg cgccggcgcg ccggccgtct gcgccgtgac gacgaacgtc accccgtcgc
      961 cgccccagcc ccaactctgc gggtcgagcg cggcgcggaa gcgcagaacg gccggcgcgt
     1021 cgggcagcgc cgtcgtgaag gccacggccg ccggcgggtg ggcgaacacc acgtcgccgg
     1081 cgtcgccgct atcgggcatg acgaagcgcc gcgcctcgac cggctccggc gagccgccgg
     1141 cgcgcacatc cagccgcccg tcgcccagcc aggccagcac atcgacgacc gtcagcaccg
     1201 ccagcgcgcc ggcgtcgccg gggtcgaggg ccagcagggc cgcggcggcg cggtcggccg
     1261 cggcggcgtc gcccgcggcc tgggcggcgg ccaactcggc gcgcaggacg gccgtctgcg
     1321 cctcgcgggc ggcggcgtcg gccgggcagt cgggcgagcg cgccgccagc gccgccgacg
     1381 ccgcgccggc gtcgccgcgg gccaggtgcg tctgcgccaa tagcggccac agggcgcact
     1441 gcgccggcgg gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggccac
     1561 gaccggcgcg tagaggttca gcgccagggc cgacacacgc ccgcgcgccc cggccagcgc
     1621 cgccaactca tcagcggtca ggtgattgcc gtcgagcagc cggaagccgg ggtcgagata
     1681 gatcggggcg acgacgccgg cggcgttgaa gccgtcgccg gtgtccaggg caatggtcag
     1741 gacgacgtcg ccgggcgcgg ccggccccag ttgcgccgcc gcgccgcgcc agtcgtggac
     1801 catctggcgg tactcggcgg ccacggccgg cgcggccacc agcaggatgg ccagggccac
     1861 ggccgcgccg gccgccgccc ttgcccgcgg ccgccggaca agggccaggg ccgcgtcgat
     1921 gcccgccccg gcggcgaaca ggtagatcgg cagcagatag atgacgtact tgggcagggc
     1981 gaagcgggtg atggccagcg cctggatgag ggccacgggc acgacggcgc acacagccag
     2041 cagcgcggcc acccccggcc gccgccgcaa cgccacggcc ccgaccgcgg ccaatagggc
     2101 cagcgccgcc gccggccacg gtcggaaaaa gccgaactcc agccacagcg ctcgcagcca
     2161 gaccggcagt gccgcggccg cggtcgtgcc cgccgcgccg ccggtgttgg cccgcagggc
     2221 gtccgcggcc cggccgagca ggagcaacag ggtcacgccg atgatcagcg ccgccggcag
     2281 cgcggccagc gcggcgcgcc gatcgccttg ccgcgcccgc caggccagcc acagggccac
     2341 ggctgcgccc tgcccggcca gccatagcca ggcgctatag tgggtcaaca ggttggccgc
     2401 ggtggcggcg gcgaaggcca gccaagtgcg tcccgcccgc ccgtcgccac gcgcgcccgc
     2461 ggctgtggcc cgatagagcc agtagagcac cagcagcgaa aagaagagca acagggcata
     2521 gtggcgggct tcctgcgagt agcggatgtg gaagggcgac accgccagca gcagcgccgc
     2581 ccagcgcccg gctgccggca atcgcgccgc ccggccgaag gccaacagca gcggcaccgc
     2641 cagcaccccc gccagcgccg agacgagccg caacgtcact tcgtgctctg gcaacacgcg
     2701 cagaacgagc gccgtcagcc agtagagcag cggcacgtgg tcgcgcacgg ccagcgccgc
     2761 cgcgggcgag gccaggctgg ccacggccgt gccgcgcgtc agcacttcgt cgaaccacag
     2821 gctatcatga ccgagataga ggaagcgcag cagggcggcc agcagggtga tggccgtggc
     2881 gatgggcagg gcagttggag cgcggtcgcg ggtcgccatc gttggcgagt ataccacgac
     2941 agaaagggac gccgacgaca cggacggcaa caggtcagga attcgttttc attcctggcg
     3001 gcgtcaatcc cgcttcagcc ggtataaccg tgtccgattt ggcccagccg cgcggccgcg
     3061 ggcgagcaaa gcgtgttcaa cagggcgacg agatcggtcg gcggctggtt ggtgtcgatg
     3121 aaacagtccg cgccggcctc cagcgctgcc cgccgcgtgg cggcgtggtg gcccagggcg
     3181 atgatgacca gttccggccg cacgctgcgc acgcgggcga cgacggccgc ggccggcagc
     3241 cccggcagcg cccagtcgag caacagcagg tccgggttct cgctcagcgc ggccagcgcg
     3301 gcctgtgcgt cggtggcttc ggtgacgcgc tcgcacagcg gatcgtgttc gagcaaagcg
     3361 cgtagggccg cccgcgtaga cggccgcgcc tcagcgatg
//
LOCUS       DAOSMQ010000353         3392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28054_length_3392_cov_4.007492, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000353 DAOSMQ010000000
VERSION     DAOSMQ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28054_length_3392_cov_4.007492"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1027)
                     /locus_tag="PK829_11090"
     CDS             complement(29..1027)
                     /locus_tag="PK829_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU41799.1"
                     /translation="MNDLLRSLVEAYGPSGFEDAVRDLIRPLVEPHADAVSVDAMGNL
                     LVHKGPAAEGALKVMIAAHIDEIGVMVTHITAKGFLRFTNIGGVMAHTLLGNRVRFAD
                     GTIGVIGVDHSDDRTVVQPLSKHFIDVGATSREDCPVRVGDAAHFDRAFVTRGNYLTA
                     KSMDDRIGCYVAIEALRRLPAGAPHDVTFVFCVQEEVGTRGAEAAANRILPDVGIALD
                     VTRTGDVPEAKAMSVSLGDGPAIKVRDSGMIAHAGLVRLMRRRAEEAGIRYQMEVLEG
                     GSTDARAMQIAGPGCAAGCISIPCRYVHSPSETVDSRDVEGSIQLLVALLSGPIDL"
     gene            complement(1048..2097)
                     /locus_tag="PK829_11095"
     CDS             complement(1048..2097)
                     /locus_tag="PK829_11095"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU41800.1"
                     /translation="MNQLLQQLTEAVGVSGGEKEVRLLLRDLIVDHVDEWSVDAMGNL
                     LATKRGTGAMDMRVLVDAHMDEVGLLITEIESGGMLCFARVGGFDDRALLGKVVQIGP
                     QKVIGIIGARAIHLLKSGEYGKTVKADAMRIDIGAKNKDGAAGKVRVGDYAAFVTAYE
                     ENTAVGGRTAIGKAFDNRAGCAALVELLRGERYPFDLVAAFTVQEEVGLRGAKIAAYS
                     ARPDAALVLECTPAYDLPDEADVSPNVALGQGASVYVMDARTIQDPRLVAHLTRTADA
                     RGIAYQVRQPGGGGTNTGAIQRGAAGIPVATLAIPGRYAHTPTMMISLDDYDSVVRLA
                     EAALRDLTPEIVRRS"
     gene            complement(2094..3197)
                     /locus_tag="PK829_11100"
     CDS             complement(2094..3197)
                     /locus_tag="PK829_11100"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOU41801.1"
                     /translation="MDTFDLLKRLTETPGPSGNEHAIAATLQELWRPLVDETTIDRVG
                     SLVAIKRGAGDSEPRPRILLAAHADELGLMVTNIVAHGGYGFLRVTNLGGVDRRHLMG
                     QTVVVHGREALTGILGGLPDARLPEEARGQAYTYDNLVLDVGLPIDRLRELVAVGDFV
                     SFRQPLRRLQKNRVAGKALDNRSSLAAVTLCLEALQARHHAWDVVAVATAQEETVYLG
                     AYTSGHAQRPDIAVAIDVTHAKGPGANDGNLAELGSGPVLGLGAEIHAAVYQGLRAAA
                     EALEMSVSVEANASGGGTDAYALQTAREGIPTGLVEIPLRYMHTMVETVDVGDVERVG
                     RLLAEMIARLDDDFLPNLRAELMRPDNRKDNGQ"
BASE COUNT          502 a         1131 c         1172 g          587 t
ORIGIN      
        1 cctggcgtct tggcgttagc ttcttctctt agaggtcgat tgggccgctc aggagggcga
       61 cgagaagctg gatggagcct tccacgtcgc ggctgtcgac ggtctcgctg ggggagtgga
      121 cgtagcggca gggaatggag atgcagccgg cagcacaacc ggggccggca atctgcatcg
      181 cgcgggcatc ggtagacccg ccttcgagca cttccatctg atagcggatg ccggcttcct
      241 cggcgcggcg gcgcatgagg cgcaccaggc cggcgtgggc gatcatgccg ctgtcgcgca
      301 ccttgatggc cgggccgtcg cccaggctaa cgctcatcgc cttggcctcc ggcacgtcgc
      361 ccgtccgtgt cacgtccagg gcgatgccga catcgggcag gatgcggttg gcggccgcct
      421 ccgcgccgcg cgtgccaact tcttcctgga cgcagaagac gaaagtcacg tcgtggggcg
      481 cgccggccgg cagccgccgc agcgcctcga tggccacata gcagccgatg cggtcgtcca
      541 tgctcttggc cgtcaggtag ttgccgcggg tgacgaaggc gcgatcgaag tgggccgcgt
      601 cgcccacgcg cacgggacaa tcctcacggc ttgtcgcgcc cacgtcgatg aagtgcttgc
      661 tgagcggttg gacgacggtg cggtcgtcgc tgtgatccac gccaatgacg ccaatcgtcc
      721 cgtcggcgaa gcgcacgcgg ttgcccagca gggtgtgggc catgacccca ccgatgttgg
      781 tgaaacgcaa aaagcctttc gcggtgatgt gggtcaccat caccccaatc tcgtcgatgt
      841 gggcggcgat catcaccttc agcgcgccct cggccgccgg ccccttgtgg accagcaggt
      901 tgcccatcgc atcgacgctg acggcgtcgg cgtggggttc caccagcggg cgaatgagat
      961 cgcgcacggc gtcctcgaaa ccggacgggc cataggcttc taccagggag cggagcagat
     1021 cgttcatagg tgtagtccct ttgtcgttca cgagcggcgc acgatctccg gcgtcaggtc
     1081 gcgcagggcg gcctcggcca ggcggacgac gctgtcatag tcgtcgaggc tgatcatcat
     1141 cgttggcgtg tgggcgtagc ggccggggat ggccagcgtg gccacgggga taccggccgc
     1201 gccgcgctgg atggcgccgg tattcgtgcc gccgccgccg ggctggcgaa cctggtaggc
     1261 gatgccacgg gcgtcggccg tgcgtgtcag gtgggccacc agccgcggat cctggatggt
     1321 gcgggcgtcc atgacgtaaa cggacgcgcc ctggccgagg gccacgttgg ggctgacgtc
     1381 cgcctcgtca ggcaggtcgt aggcgggcgt gcactccagc accagcgccg catcgggccg
     1441 ggcgctgtag gcggcaatct tggccccgcg cagaccaact tcctcctgca cagtgaaggc
     1501 ggccaccagg tcgaacgggt agcgctcgcc gcgcaacaac tccaccagag ccgcgcagcc
     1561 ggcgcggttg tcgaacgcct tgccgatggc cgtccgccca ccaacggcgg tgttctcctc
     1621 ataggcagtg acgaaggcgg cgtagtcgcc caccctcacc ttgcccgccg cgccatcctt
     1681 gtttttcgcg ccgatgtcga tgcgcatggc gtcggccttc acggttttgc cgtactcgcc
     1741 cgacttgagc agatggatgg cccgcgcgcc gatgataccg atgacctttt gcgggccgat
     1801 ctggacgacc ttgcccagca gggcgcggtc gtcaaagccg cccaccctgg caaagcacaa
     1861 catgccgccg ctttcgatct cggtgatcag caggccgacc tcgtccatgt gggcatcgac
     1921 gaggacgcgc atgtccatcg cgcccgtgcc gcgcttggtg gccagcaggt tgcccatggc
     1981 gtcgaccgac cactcatcga cgtggtcgac gatgaggtcg cgcaacagca agcgcacttc
     2041 cttctcgccg ccggagacgc cgacggcctc cgtcagttgt tgcaggagct ggttcattgg
     2101 ccattgtcct tgcggttgtc cggccgcatc agttccgcgc gcaggttggg caggaagtca
     2161 tcgtccagcc gcgcgatcat ctcggccagc aggcggccga cgcgctccac gtcgcccaca
     2221 tcgaccgtct ccaccatcgt gtgcatgtag cgcagaggaa tctccaccag gccggtgggg
     2281 atgccctcgc gggccgtttg caaagcatag gcgtccgtgc ctccgccgct ggcgttggcc
     2341 tccacgctaa ccgacatttc cagcgcctcg gcggcggcgc gcaatccctg atagacggcc
     2401 gcgtgaatct ccgcgcccag acccagcacc gggccactgc ccagttcggc caggttgccg
     2461 tcattggcgc ctggcccctt ggcatgggtc acatcaatgg ccacggcgat gtcggggcgc
     2521 tgggcgtggc cgctggtgta tgcgccgagg tagacggtct cttcctgcgc cgtggccacg
     2581 gcaactacgt cccaggcgtg gtggcgagcc tggagagcct ccagacagag cgttaccgcg
     2641 gccagcgagg agcgattgtc cagcgccttg ccggcgacgc ggttcttctg caggcggcgc
     2701 agcggctggc ggaagctcac gaagtcgccc accgccacca actcgcgcag ccggtcaatg
     2761 ggcaggccca cgtcgaggac gaggttgtca taggtgtagg cctgaccacg cgcctcctcc
     2821 ggtagccgtg catcgggcag gccgccgagg atgccggtca gcgcttcgcg gccgtggacg
     2881 acgaccgtct ggcccatcag gtggcggcga tcgaccccac cgaggttggt cacgcgcagg
     2941 aagccgtagc cgccgtgcgc gacgatgttg gtgaccatca accccagttc gtcggcgtgg
     3001 gcggccagaa ggatgcgcgg ccgcggctcg ctatcgcctg cgccgcgttt gatggcgacg
     3061 aggctgccga cgcgatcgat ggtcgtctca tcgaccaacg gccgccacag ttcttgcagc
     3121 gtggcggcga tggcatgttc gttgcccgac ggtcccggcg tttctgtcag gcgtttcagg
     3181 agatcgaaag tgtccatgag cgctagtgat ccttgtccgg tcagggcgtc gccgtcgccg
     3241 tcgggctggc cgaggggtcg atggtcggcg tctcgctggg cagcacgtcg aagtccaggg
     3301 tcggcgtagc tgacggcgtc tcggtggctg tcggggtctc ggtcggcgtc tcggtgctgg
     3361 tcactgccgc cgtgttgccg atgtcacctc gc
//
LOCUS       DAOSMQ010000354         3377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28208_length_3377_cov_4.619807, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000354 DAOSMQ010000000
VERSION     DAOSMQ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28208_length_3377_cov_4.619807"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PK829_11105"
     CDS             complement(<1..560)
                     /locus_tag="PK829_11105"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOU41802.1"
                     /translation="MSHPNTAGARRYLFLTVFVAGMCTLAVEFTASRLLQTVYGTSNI
                     VWANVIGLVLLSLTVGYFAGGRLADRRPGASLFYTLVSLAGFAAVFFLLLTSVILRGA
                     AGALAVMNVGAVAGSLLLVVLALVVPVTLLGCLSPFAVRLAVRDVAEAGRVSGRIYAV
                     STLGSLLGTYLPVLVVIPLAGSRAAAV"
     gene            complement(557..2584)
                     /locus_tag="PK829_11110"
     CDS             complement(557..2584)
                     /locus_tag="PK829_11110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU41803.1"
                     /translation="MSRAERAALAFILLVAAALRLTGLDWDGFQHHHPDERYITWVAT
                     TIEFPRPGSMSRADWQPQTSTFNPFHWPPAASSEGIVVLQDEPRDFAYGHLPLYLGVL
                     ATRFVERLGPLLIPLLPAGWSLTGDVLNGAGRIEFHHLAIVGRALTALFDVGTVGVVF
                     LLGRRLYGVGVALLASAFLAVAVLPIQLAHFFTSDPYLTFFAVAALYALVAGHGAGDA
                     RRRQRWWLAAGALIGLAVGSKFSAILLFAPLLWAIGSGERAGRRWAEGLAAVAVAVLV
                     FALTNPFALLDRTCPAPAEWPRLGPLAAPEALVRSCYLQNLAEQSAMARGTADLGFTR
                     QYTGTVPYLYFIEMQIRWGLGPLLGVVAFAGLGWAIWGLLWPPGAAGSDGWTRLRREP
                     VVIPLLWVVPYFLITGAFAVKFMRYMQPILPLLVLFGAAMLWAGGRRWQERRATARPG
                     RWAGGVAGIVLAFTALYAAAFMAIYSTEHPWNAASRWVYEHVPPGTTLLSEQWDDYLP
                     VPLLLDGQRHLRNEYPNVELTWLSRPDAADDEAKLRANLEALAAADYVTILSNRVYGV
                     VPRLPDRYPLSSRYHQLLFDGELGYELAWVGGRAPRLFGLTVRLDTFAWPGLTPPAAV
                     AAYLRAGPALSFGRVDESFVVYDQPLTMIFRNTGRLTAEQMRAAFDAGATD"
     gene            complement(2593..3231)
                     /locus_tag="PK829_11115"
     CDS             complement(2593..3231)
                     /locus_tag="PK829_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOU41804.1"
                     /translation="MSERIVIEAEPREITGKQVSQLRREGWIPGVIYGRKEPVSVQME
                     QKALRRALRTVGTTHLADVNVGGQMRTVLVREIQQHATRGDLVHVDFMEVDMKSKLRA
                     TAELVGVGMSTPQSQGLGVTTMMLREVDIECLPENLVAEIEVDLSAIQAADDVIHVRD
                     LTAPTGVEILSDPELMVARFEFAASDEEEAGEVEAEADVEVITKGKKEEEDF"
BASE COUNT          643 a         1155 c         1103 g          476 t
ORIGIN      
        1 accgccgccg cccgcgaccc ggccagggga atgaccacca gcacgggcaa atacgtcccc
       61 agcaggctgc ccagggtgga gacggcgtag atgcgcccgc tgacgcgccc cgcctcggcc
      121 acgtcgcgca cggccaggcg tacggcgaac ggcgacaggc agcccagcaa cgtcaccggc
      181 acgaccaggg ccagcacgac caggagcagc gagccggcca ccgcgccaac gttcatgacc
      241 gccagcgccc cggccgcgcc gcgcaggatg acgctggtca gcagcaaaaa gaagacggcg
      301 gcgaagccgg ccaagctgac cagggtgtag aacaggctcg cgcccggtcg ccggtcggcc
      361 agccgcccgc cggcgaagta gccaaccgtt agcgacagca ataccaggcc gatgacgttg
      421 gcccagacga tgttggacgt gccgtagacc gtctgcaaga gacgactggc tgtgaactcg
      481 acggccagcg tgcacatgcc ggcaacgaag acggtcagga agagatagcg gcgcgcgccg
      541 gccgtgttgg ggtggctcaa tctgtggctc ctgcgtcaaa ggcggcgcgc atttgctcgg
      601 ccgtcaggcg acccgtgttg cggaagatca tcgtcaacgg ctggtcgtaa acgacaaagc
      661 tctcatccac ccggccaaac gacagcgccg gcccggcgcg gagataggca gccacggccg
      721 cgggtggggt caggcccggc caggcaaagg tgtcgagccg gacggtcagg ccgaacagcc
      781 gtggcgcgcg gccgcccacc cacgccagtt cgtaccccag ttcgccgtcg aacagcagct
      841 ggtgatagcg cgacgacagc ggatagcggt cgggcaggcg cggcacgacg ccataaacgc
      901 ggttggagag gatggtcacg tagtcggccg cggccagcgc ttccagattg gcccgcagct
      961 tggcctcgtc gtcggctgcg tcggggcgcg agagccaggt caactcgacg ttggggtact
     1021 cgttgcgcag gtggcgctgg ccgtccagca gcagaggcac cggcagatag tcgtcccact
     1081 gctccgacaa cagtgtcgtc cccggcggca cgtgctcata gacccagcgc gaggccgcgt
     1141 tccagggatg ttcggtgcta tagatggcca tgaaggcagc ggcgtagagg gccgtgaagg
     1201 ccaaaacgat gccggccacg ccccctgccc aacgacccgg ccgcgccgtg gcccgccgct
     1261 cttgccaacg ccgcccgccg gcccagagca tggccgcacc gaagagcacc agcagcggca
     1321 gaatgggctg catgtagcgc atgaacttga cggcaaacgc gccggtgatc aggaagtagg
     1381 gcacgaccca tagtagcggg atgacaaccg gctcgcgccg cagccgcgtc cagccgtcgc
     1441 taccggccgc gcccggcggc cagagcagtc cccagatagc ccagcccagt ccggcgaagg
     1501 cgacgacgcc cagcagcggc cccaggcccc aacgaatctg catctcgatg aagtacagat
     1561 acggcacggt gcccgtatat tggcgcgtga agcccagatc ggccgtgccg cgcgccatgg
     1621 cgctttgctc ggccaggttc tgcaagtagc acgagcgcac gagggcctct ggcgcggcca
     1681 gtgggccaag gcgcggccat tccgccggcg cggggcaggt gcggtcgaga agcgcgaagg
     1741 ggttggtcag ggcaaaaaca aggacagcca cggccaccgc tgccaggccc tccgcccatc
     1801 gtcgccccgc ccgttcgccg ctgccaatag cccacaacag cggcgcaaag agcaggatgg
     1861 ccgagaactt cgaccccacc gccaggccga tcagcgcccc ggcagccagc caccatcgct
     1921 gccgccggcg cgcgtcgccg gccccgtggc cggcaaccag ggcatagagc gcggcgacgg
     1981 cgaagaaggt cagatagggg tcgctggtga agaagtgggc caactggatg ggcagcacag
     2041 ccacggccag aaaggcagac gccagcagag cgacgccgac gccatagagc cgccggccaa
     2101 gcaggaacac tacccctacc gtgcctacgt cgaacagcgc cgtcaaggcc cggccgacaa
     2161 tggccagatg atggaactcg atgcgccctg cgccattcaa cacgtcgccg gtcagcgacc
     2221 agccggccgg cagcaacggg atgagtagcg gccccagtcg ttcaacaaag cgcgtagcca
     2281 agacccccag gtagagcggc aggtggccat aagcgaagtc gcgcggctcg tcctgcaaaa
     2341 cgacaatccc ttcgctgctg gccgcgggcg gccagtggaa gggattgaac gtcgatgttt
     2401 gcggttgcca gtcggcccgg ctcatggagc cgggccgcgg gaattcgatt gtggtcgcta
     2461 cccaggtgat gtagcgctcg tccggatggt gatgctggaa gccgtcccag tccagtccgg
     2521 tcaggcgcag ggcggccgcg accagcaaga taaaggccag ggcggcgcgc tcggctcgag
     2581 acacggcccg gtctagaagt cctcttcttc cttcttgccc ttggtaataa cttccacgtc
     2641 ggcctcggcc tcgacctcac cggcttcctc ttcgtcgctg gcggcaaact cgaagcgcgc
     2701 caccatcagc tcgggatcgg acagaatctc gacgcccgtg ggcgcggtca ggtcacggac
     2761 gtggatgacg tcatcagccg cctggatggc gctcaggtca acctcgatct cggccaccag
     2821 gttttccggc aagcattcga tgtccacttc gcgcaacatc atcgtggtca cacccaggcc
     2881 ctgagactgg ggcgtagaca tgccgacgcc caccagctcg gctgtggccc gcagcttgct
     2941 cttcatgtcc acttccataa aatcgacgtg gacaaggtcg ccgcgcgtgg cgtgctgctg
     3001 aatctcgcgc accagcaccg ttcgcatttg accgccaacg ttgacgtcgg ccaggtgggt
     3061 tgtgcccacg gtccggagag cgcgccgcag cgccttctgc tccatttgca ctgagaccgg
     3121 ctccttgcgc ccgtaaatga cgcccggaat ccagccctcg cgccgcaact gactaacctg
     3181 cttgccggtg atttcgcggg gttccgcttc gattacgatt ctctcgctca tgcctctgtg
     3241 ttccttcctg gagcaacagc caaacgggtg tcgcgcgtcc gtcttgcgca cggacttgta
     3301 cgtcgtactt gtgcgccaca caagtgtacc acgcttgtgt gccacatttg tgcgccacac
     3361 aagtgtgcca cacttgt
//
LOCUS       DAOSMQ010000355         3358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28410_length_3358_cov_5.003633, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000355 DAOSMQ010000000
VERSION     DAOSMQ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28410_length_3358_cov_5.003633"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PK829_11120"
     CDS             complement(<1..1069)
                     /locus_tag="PK829_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU41805.1"
                     /translation="MGVLLDKVWFDLWHRKARTLLAVLSIAAGVFAVGAMFGLTDQML
                     SGMDAAHRAVNPSHLNIILRRPIDRATAEALTDIPGIVDVEPMNLRTMRYKTSPDETE
                     WQAATVVARDDYENQIFDYLTLTAGEWPGNKHIAVERITSSYYGIDLGDEIIFEMDGT
                     DRAYPVTGLIRHPFVPPPQFGGNAYFFVDSEMMARFGLLEGQFVQLLVQVEPYSEDYA
                     RDRAAAIKQELARQDVGVSLVIYQEPDKHWGHDTVLGITLVLQILAVVALFTSVVIVI
                     NTTTAIITQQTDQIGVIKALGGTTRVVARVYLLGVLIFGLLALVIALPAGMAMAFLTT
                     RQLLLLFNIDYVGFRFSTRAVI"
     gene            complement(1085..1843)
                     /locus_tag="PK829_11125"
     CDS             complement(1085..1843)
                     /locus_tag="PK829_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41806.1"
                     /translation="MDNANGRPAPIVSVRDVYKRFPAGETEVTILKGVSLDIYPAEFV
                     AIVGPSGNGKSTLLNMITGIDHPSEGQVIVTGQSVHAMSENELAVWRGQHLGIIFQFF
                     QLLPALSLVKNVMLPMDLVNQRPRRERAERAMALLDMVGLADQAHKLPSQVSGGQQQR
                     AAVARALANDPPLLVADEPTGNLDSTTAENVFQLFLKLVAGGKTLVMVTHSRTLAARG
                     SRVVEIRDGLIAHDSSAAARPQRSSATPAVSLTY"
     gene            2037..2666
                     /locus_tag="PK829_11130"
     CDS             2037..2666
                     /locus_tag="PK829_11130"
                     /inference="COORDINATES: protein motif:HMM:NF040089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41807.1"
                     /translation="MAIVPNELLVELRNTFRNRLSLSELADLCQDLGLNIENLPGTSL
                     SEKSRELAAYIGRRDLVGKLATVGPVLLPDIPWLEILGRYGYGPQPDAGNSDGSAPPA
                     TAVSTEDLQRLSPILAHHSEFNTPGARRDMLAIAGVLSYLDVDLSGSARQVANAVLLR
                     LNDRGQIAPGDYALGRLLKYLLDDDTLPEGARATLVQIVARYGLNQAAG"
     gene            2690..>3358
                     /locus_tag="PK829_11135"
     CDS             2690..>3358
                     /locus_tag="PK829_11135"
                     /inference="COORDINATES: protein motif:HMM:NF040089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41808.1"
                     /translation="MAEGFLTLTDKQQLARVLANVYDLKMGPAARASFLDTAGLGRFV
                     TAVNLAAEAPRTLAQELVTRLEPFGMLPAVPSQPQYHALGALVDYVRQMADTNNADAR
                     FLAEMMVKYQLIADTAYLARLKADYQLDTEPVRQTDPALSPPSEPPAPADEPPFDPTV
                     GDQAELELIVNSEDNFIDIDMLAGAIYTAQAVGRIERPSGRALGTGFLVGPDLMLTNY
                     HVFKD"
BASE COUNT          591 a         1070 c         1129 g          568 t
ORIGIN      
        1 agatcaccgc ccgcgtcgag aaacggaagc cgacgtagtc gatgttgaac agcaacagga
       61 gttgcctggt ggtcaggaag gccatggcca tgcccgccgg cagggcgatg accaacgcca
      121 acaggccgaa gatgagcacg cccagcaggt agacgcgggc caccacgcgc gtcgtgccgc
      181 ccagcgcctt gatgacgccg atctggtcgg tctgctgggt gatgatcgct gtcgtcgtgt
      241 tgatgacgat gaccacgctg gtgaacaggg cgacgacggc caggatttgc agcacaaggg
      301 tgatgccgag gacggtgtcg tggccccaat gcttgtccgg ctcctgatag atgaccagac
      361 tgacgccgac gtcctgccgc gccaattcct gcttgatagc cgcggcgcgg tcgcgggcat
      421 agtcctcgct atacggctcc acctggacga gcaactgaac gaattgcccc tccagcaggc
      481 cgaagcgggc catcatctcg ctatcgacga agaagtaggc gttgccgccg aactggggcg
      541 gggggacgaa cgggtggcgg atgaggccgg tgacggggta ggcgcggtcg gtgccgtcca
      601 tctcgaagat gatctcgtcg cccaggtcga tgccgtagta gctgctggta atgcgctcga
      661 cggcgatgtg cttgttgccg ggccactcgc cggccgtcag cgtcaggtag tcgaatatct
      721 ggttctcata gtcgtcgcgg gcgacgaccg tggccgcctg ccactcggtc tcgtcgggcg
      781 aggtcttgta gcgcatcgtc cgcagattca tcggctcgac gtcgacaatg ccggggatat
      841 cggtcagcgc ttcggccgtg gcccggtcga tgggccggcg caggatgatg ttgaggtgcg
      901 acgggttgac ggcgcggtgg gcggcatcca tgcccgacag catctggtcg gtcaggccga
      961 acatggcccc cacggcgaac acgcccgcgg cgatgctgag cacggccagc agcgtgcgcg
     1021 ccttgcggtg ccagaggtca aaccagacct tgtcgaggag gacgcccatg gctgaagaga
     1081 gtggtcagta ggtcagggag accgctgggg tcgcagaaga cctctgtggt cgagcggccg
     1141 cgctgctatc gtgggcgatc aggccatcgc gaatctcgac cacgcgcgag ccgcgggcgg
     1201 ccagggtgcg gctgtgggtg accatgacca gcgtcttgcc accggcgacc agcttcaaga
     1261 aaagctggaa gacgttctcg gccgtggtcg agtccaggtt gcccgtcggc tcgtcggcca
     1321 ccagcagcgg cgggtcgttg gccaacgcgc gggcgacggc ggcacgctgc tgctggccac
     1381 ccgacacctg gctgggcagc ttgtgggcct ggtcggccag gcccaccata tccagcaggg
     1441 ccatggcccg ctcggcccgc tcgcgccgcg gccgctggtt gaccagatcc atcggcagca
     1501 tgacattttt gaccaggctg agggccggca atagctggaa gaactggaag atgatgccca
     1561 agtgctggcc gcgccagacg gccagttcgt tctcgctcat ggcgtgcacc gattggccag
     1621 tgacgataac ctgcccctcg ctggggtggt cgatgccggt gatcatgttc agcagcgtcg
     1681 atttgccgtt gcccgacggg ccgacgatgg ccacgaactc agcgggatag atgtcgagcg
     1741 agacgccctt gaggatcgtc acctccgttt cgccggcggg gaagcgtttg tagacatcgc
     1801 gcaccgagac gatgggcgcg ggtcggccgt tggcattgtc catagccggc cagtttaaca
     1861 gcattcgtgc taaaaatcac aagcgcgggc tgccctctca tatgcacgac ggctctgtaa
     1921 aaagacccac caggagcctc gctttcgttg aagagatcgc cgactttatg ttattatttg
     1981 ccaagtccgg cgcagttgtg ccagacagaa acccttggcc gcgagaggag tgtcgcatgg
     2041 ctatcgtgcc gaacgagtta ctggtcgagt tgcgcaatac cttccgcaac cgcctgagcc
     2101 tgagcgagct ggccgatctg tgtcaggacc tgggcctcaa catcgagaac ctgcccggca
     2161 cgagcctgag cgagaaatcg cgcgaactgg cggcctacat cggccggcgc gacctggtcg
     2221 gcaagctggc cacggtgggg ccggttttgc tgccggacat accctggctg gagattctgg
     2281 gccgctacgg ctacgggcca cagccggacg cgggtaacag tgacggcagc gccccgccgg
     2341 ccacggccgt cagcaccgag gacttgcagc gcctctcgcc catcctggcc caccactccg
     2401 aattcaacac gcccggcgcg cgccgcgaca tgctggccat cgccggcgtg ctgtcgtatc
     2461 tcgacgtcga tctgtccggt tcggcgcggc aggtggccaa cgccgtcctg ctgcgcctca
     2521 acgaccgcgg ccagatcgcg ccgggtgact acgcgctcgg ccgtctgctg aagtatctgc
     2581 ttgacgatga caccttgccg gagggggcgc gggccacact cgtccagatc gtcgcccgct
     2641 atgggctgaa tcaggctgcc ggataagggg cagctaagga gggattgcga tggccgaagg
     2701 atttttgaca ctgacagaca aacagcaact ggccagagtg ctggccaacg tttacgacct
     2761 gaagatgggg ccggcggcgc gggccagctt cctggatacg gccgggttgg gccgcttcgt
     2821 gaccgccgtc aacctggccg ccgaagcgcc gcggacgttg gcccaggagc ttgtcacccg
     2881 gttggagccg tttggcatgt tgccggccgt gcccagccag ccgcagtacc atgctctggg
     2941 cgcgctggtc gattacgtcc gccagatggc cgacaccaac aacgccgacg cccgcttcct
     3001 ggccgagatg atggtcaagt accagctcat cgccgatacg gcctatctgg cgcgactgaa
     3061 ggcggactat cagctcgaca cggagccggt gcgccagacc gacccggccc tgtcgccgcc
     3121 gagcgagccg ccggccccgg ccgacgagcc gccttttgac cccactgtgg gcgaccaggc
     3181 cgagctggag ctgatcgtca actccgagga caacttcatc gacatcgaca tgctggccgg
     3241 ggcgatctac accgcccagg ccgtcggccg tatcgagcgg cccagcggtc gcgccctggg
     3301 cacgggcttc cttgtcggcc ccgacctgat gctgaccaac taccacgtct tcaaggac
//
LOCUS       DAOSMQ010000356         3352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28474_length_3352_cov_4.929633, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000356 DAOSMQ010000000
VERSION     DAOSMQ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28474_length_3352_cov_4.929633"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK829_11140"
     CDS             <1..204
                     /locus_tag="PK829_11140"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOU41809.1"
                     /translation="SRTQIGQLTSHAFSPMLKQYVGIGQLLTPAAPMGGAVDVEVTVE
                     FKRKLCPARIVPTPFFDPPRKRG"
     gene            286..1878
                     /locus_tag="PK829_11145"
     CDS             286..1878
                     /locus_tag="PK829_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU41810.1"
                     /translation="MTNQHYDAIVIGAGHNGLVAGAYLARSGKKTLILEKRHLVGGAS
                     VTEEIYPGFKFTVFSYVVSLFRPEIVRELNLPAHGLTILPLESTLTPTLDGDDYLYRD
                     GDHYQTLRNIARFSPRDADAYDDYARSMYFMAKAVKYILGVIPPDPTTLHPKDMMGLL
                     EMARHFLGLGQEQLYMLAKLMTMSAADFVEQWFESEPLKATLSASGIIGTFLGPRSPG
                     TAYVLLHHYMGEIDGAYRAWGFHKGGTGGLAEVLAQAARAQGAEVRCNAGVARVLTSG
                     GRAAGVVLENGDEIRAPLVLSSLDPKQTFLRLVDPQQLPDDLVVAVRRFQTFGSSGKV
                     NLALDRLPELGCRPGVGRHLAGAVSISPNLDYIERAYDDAKYGHFSRRPYVDIIIPSM
                     IDPDMAPPGKHVMSCFVQYAPYELADGDWTDARRDAFGDAVVDVLATYFPNIRDLILH
                     RQVLTPVDVERITGITQGNIFHGELNLSQLFFLRPAPGYAKYRTPIKGYYQCGSGTHP
                     GGGIMGAPGRLGALEALRDSRS"
     gene            1881..>3352
                     /locus_tag="PK829_11150"
     CDS             1881..>3352
                     /locus_tag="PK829_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU41811.1"
                     /translation="MTVLYDTIIIGAGHNGLVAAATLAAGGQRVLVLEQRPALGGVAG
                     AEEAWPGYYVPTGATDAALLQEEIIRKLDLGRHGLAFAQPPALLFAPQPDGRSLTIWR
                     DEARTTAEIARLSRHDAERWPAFRAQVERVARLLSGMLLLAPPDLSALRGGDLKGWAP
                     LALRLKRLGDADMMELMRILPMAVRDYLDEWFESDVLKGALGGSAVVGATLGPRGAGT
                     NLGFLYQNLGGLLQVRPVAGGMGALAAALAAAARARGAEIRTGAAVERILIEGDLEPV
                     AAGVVLHSGETIRAGVVLSNADPRRTLFDLVGPQHLEPETMRAVRNIIYRGSTARVDL
                     ALNGLPDFAGATGTEQLGGRIRVSPSLDYIERAYDAAKYGRASERPYLEIVIPTLNDP
                     SLAPAGHHILTITALYAPYALRDGDWAGQAPAFGDAVLDVLEGVAPGTRATVVHCRVV
                     TPLDLEQRYGLTEGSIYHGQMGLDQMLVMRPIPGWSRYETP"
BASE COUNT          536 a         1210 c         1070 g          536 t
ORIGIN      
        1 agccgcacgc agatcggcca gctaaccagc catgccttct cgcccatgct caagcaatac
       61 gtcggcatcg gccagctgtt gacgccagcc gcgcccatgg gcggggcggt tgatgtcgaa
      121 gtgacagtcg agttcaagcg caagctctgc ccggcgcgga ttgtgccgac gccgttcttc
      181 gatccgccgc ggaagagggg ctaggttcca ggttccaggt tccagggaag agcgctcttc
      241 cctggaacct ggaacctgga acctagcccc taaaaagagg agaccatgac caaccaacac
      301 tacgacgcca tcgtgatcgg cgccggacac aacggcctgg tggccggggc ctatctggcg
      361 cggtcaggca agaagacgct cattctggag aaacgccacc ttgtcggcgg cgcgtcggtg
      421 accgaagaaa tctaccccgg cttcaagttc accgtcttct cctacgtggt cagccttttc
      481 cggccggaga tcgtccgcga actgaacctg cccgcccacg gcctgaccat cctgccgctg
      541 gagagcacac tgacgccgac gctggacggc gacgactacc tctaccgcga cggcgaccac
      601 taccagacgc tgcgcaacat cgcccgcttc tcgccgcgcg acgccgacgc ctacgacgac
      661 tacgcccgca gcatgtactt catggccaag gcggtcaagt acatcctcgg cgtcattccg
      721 cccgacccga cgacgctgca ccccaaggac atgatgggcc tgctggagat ggcccgccac
      781 ttcctggggc tggggcagga gcagctctat atgctggcca aactgatgac catgagcgcg
      841 gccgacttcg tggagcagtg gttcgagtcg gagccgctca aggcaacgct gtcggccagc
      901 ggcatcatcg gcacgttcct cggcccgcgc tcgcccggca cggcctacgt gctgctccac
      961 cactacatgg gcgagatcga cggggcctac cgcgcctggg gcttccacaa gggcggcacg
     1021 ggcggcctgg cggaagtgct ggcccaggcg gcccgtgcgc agggggccga ggtgcgctgc
     1081 aacgccggcg tggcccgcgt gctgacgtcc ggcggccgcg ccgccggcgt cgtcctggag
     1141 aacggcgacg agattcgcgc gccgctggtt ctttccagcc ttgaccccaa gcagacattc
     1201 ctgcgcctgg tcgatccgca gcaactgccc gatgatctgg ttgtcgcggt gcggcgcttc
     1261 caaacgttcg gttcgtcggg caaagttaac ctagccctcg accggctgcc ggagctaggc
     1321 tgccggccgg gtgtcggccg tcatctggcc ggggccgtgt ccatcagccc caatctcgac
     1381 tacatcgaac gcgcctatga cgacgccaaa tatggccact tctcgcgccg cccctacgtc
     1441 gatatcatca tcccgtcaat gatcgacccc gacatggccc cgcccggtaa gcacgtcatg
     1501 tcctgcttcg tccagtacgc gccctacgag ctggccgatg gcgactggac cgacgcccgg
     1561 cgcgacgcct ttggcgatgc cgtcgtcgat gtgctggcga cctacttccc caacatccgc
     1621 gacctgattc tgcaccggca agtcctgacg ccggtggacg tagagcgcat caccggcatc
     1681 acccagggca atattttcca cggcgagcta aacctctcac aactcttctt cctacgcccc
     1741 gcccctggct atgcgaagta tcgtaccccc atcaagggtt actaccagtg cggctccggc
     1801 acccaccccg gcggtggcat catgggcgcg ccggggcgtt tgggggcgct ggaggcgtta
     1861 cgggactcta ggagctaaca atgacagtac tctacgacac gatcatcatc ggcgccggac
     1921 acaatggcct tgtcgccgcg gcgacgctgg ccgcgggcgg ccagcgtgtc cttgtgctgg
     1981 agcagcgacc ggcgctcggc ggcgtggccg gcgcggaaga ggcctggccc ggctactacg
     2041 tccctaccgg ggccaccgac gcggctctgt tgcaggagga gatcattcgc aagctcgacc
     2101 tgggccgcca cggcctggcc ttcgctcagc cgcccgcgct gctcttcgcg ccccagcccg
     2161 acggccgcag cctgaccatc tggcgcgacg aggcccggac gacagccgag atcgcccgcc
     2221 tgagccgcca cgacgccgag cgctggcccg ccttccgcgc tcaggtcgag cgcgtggccc
     2281 ggctgctcag cggcatgctg ctgctggccc cgcccgatct gagtgccctg cgcggcggcg
     2341 acctgaaggg ctgggcgccg ctggcgctac gccttaagcg gctgggcgac gccgatatga
     2401 tggaactgat gcgcatcctg cccatggccg tccgcgacta tctggacgag tggttcgaga
     2461 gtgacgtcct caagggcgcg ttgggcggct cggccgtcgt cggcgcgacg ttggggccgc
     2521 gtggcgcggg caccaacctg ggcttcctct accagaatct cggcgggctg ttgcaagtcc
     2581 ggcccgtcgc cggcggcatg ggcgcgctgg cggcagcgtt ggccgcggcg gcgcgcgctc
     2641 gtggcgcgga gatacgcacc ggcgcggccg tcgagcgcat tctgatcgag ggcgacctgg
     2701 agccagtcgc cgccggcgtc gtcctgcaca gcggcgagac cattcgcgcc ggcgtcgttc
     2761 tgtccaacgc cgacccgcgg cggacgctct tcgacctcgt tggcccgcaa cacctggagc
     2821 cggagacgat gcgcgccgtc cgcaacatca tctaccgcgg cagcaccgcc cgcgtcgacc
     2881 tggccctcaa cggcctgccc gacttcgccg gcgcgaccgg gactgagcaa ctgggcgggc
     2941 gcatccgcgt cagccccagc ctcgactaca tcgagcgcgc ctacgacgcg gccaagtatg
     3001 gccgcgcctc tgagcgcccc tacctggaaa tcgttattcc aaccttgaac gacccgtctc
     3061 tggccccggc cggccaccat attctgacca tcactgccct ctacgcccct tatgccctgc
     3121 gcgacggcga ttgggccggc caggcacccg cgttcggcga cgccgtgctg gacgtactcg
     3181 aaggcgtggc ccccggcacg cgggccacgg tcgtccattg ccgcgtcgtg acgccgctcg
     3241 acctggagca gcgctatggc ctgaccgagg gcagcattta ccacggccag atggggctgg
     3301 accagatgct ggtcatgcgg cccattcccg gctggagccg ctacgagacg cc
//
LOCUS       DAOSMQ010000357         3338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28629_length_3338_cov_4.454462, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000357 DAOSMQ010000000
VERSION     DAOSMQ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28629_length_3338_cov_4.454462"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PK829_11155"
     CDS             complement(<1..321)
                     /locus_tag="PK829_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOU41812.1"
                     /translation="MNEKVGFIGLGIMGGAMAANLLKAGFDVTVWNRTPSRITPLASA
                     GAAVGDHPADVAGRSDIIVVCVSDTPDVEAVVLGPDGVLAGARPGALVIDCSTISPEA
                     TRAIA"
     gene            610..1914
                     /locus_tag="PK829_11160"
     CDS             610..1914
                     /locus_tag="PK829_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOU41813.1"
                     /translation="MVGKKRWLLIAVALAALAGGATWWALPRLPGRVRVYLPEPVAAL
                     VTTPLPAALPTAAVTTAAAPVATFELAQLVAEPPAAPSPTPALPTAQPAPPLAPTLAA
                     ATETPAPSLPPYGRVANIPIIPQKFNNCGPTNLTLVLNHYGIDVDQFAVAALVRPNYE
                     DRNVSPDELVDYVNEQTPLRAAAYAGGDIDTLRALLAAGVPVIIETGLEPDAATGWMG
                     HYLTVFAYDDITRHFSVRDTYLGPWQGEGLAGYDETARTWAQFNGAFVVVYPPERAAE
                     VAAALGPAFADAATMWAAAVDAARAAARARPDDPFAWFNLGSGLTALAGPGDAALMAG
                     AAAAFDRARQLGLPPRMLWYQFAPYEAYLAAGRAGDVLTLTAVTLENQGGRNVEETYL
                     YQGLALLATGDAAGARAALARAVELNAASPTGKAAQAALDGL"
     gene            complement(2042..3274)
                     /locus_tag="PK829_11165"
     CDS             complement(2042..3274)
                     /locus_tag="PK829_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41814.1"
                     /translation="MYDSLARDDFERAYQRSLWRKLITRLTGKSNQLLPFDEVRQALP
                     YRGQRDIGLQEVALDKIVGSVGRYRDFDRAFLPTQRQTTERWVNVSKARYNEVALPPV
                     ELYKIGEVYFVKDGNHRVSVARERKQAIIDAFVTEIDIPFRLTADMDIDSVQQEKAYG
                     QFVQDTRLDELRPDSDLHLSNPAEYARLREHIAAHHYYLGTERQADVSYGEAVQSWID
                     NVYRPLVDAIEAQGLRRQFPEMTAADLYLRVSEYQWLVREAAQADVGSDEARDEATER
                     MAGIYQQGVVRRVIRTLRGANWIDTMIRDKEYAAFLERTGLLALRPDARVLASLPGKY
                     EKILHHIDVHHYYLGLEQQRHVPYSEAVASWYDNVYLPTIRDIREQNLLADFPGRTET
                     DAYIWLIEHREEMEQAQP"
BASE COUNT          480 a         1099 c         1221 g          538 t
ORIGIN      
        1 ggcgatggcg cgggtggcct ccgggctgat ggtgctgcaa tcgatgacca gcgcgcccgg
       61 ccgcgccccg gccagcacgc cgtcggggcc gaggacgacc gcctccacgt cgggcgtgtc
      121 gctgacgcag acgacgatga tgtcgctgcg gccggccacg tcggcgggat ggtcgcccac
      181 ggccgcgccc gccgacgcca gcggcgtaat gcggctgggc gtgcggttcc agaccgtcac
      241 gtcgaaaccg gccttcaaca aattcgcggc catcgcgccg cccatgatgc ccagtccaat
      301 gaagccgacc ttttcgttca tgggggggat tatagctgat agaagtgggc agtgggcagt
      361 gggagcgcgc tgaccgccaa cgacggaccg ccgaccactg cctcagggtc atgacaacgt
      421 caataggctc tcacgcaaag gcgcgaaggg gcaaaaggcg caaagaagaa atggccgatg
      481 atcgagcttt ttcttggcgt ctttcgctta ctttgcggct ttgcgtgagc gctccttccg
      541 gctttgcaat gaccctgccg ctaacccact gcccctaccc attcatcgcg ctgcccgtat
      601 aatgggcgca tggtaggcaa gaaacgctgg ctgttgattg ctgtcgcgct ggcggcgctg
      661 gcgggcgggg cgacgtggtg ggcgctgccg cgactgccgg ggcgcgtgcg cgtctatttg
      721 ccagagccgg tcgccgcgct ggtgacgacg ccgctgccgg cggcgctgcc cacggccgct
      781 gtgaccacgg cggccgcgcc cgtggctacg tttgagttgg cgcagttggt ggccgagccg
      841 ccggccgcgc cctcgcccac gccggccctg cccacggctc agcccgcgcc gccactcgcg
      901 ccaacgctcg ccgccgccac ggaaacgccc gccccgtccc tgccgcccta cggccgcgtg
      961 gccaacattc ccatcatccc ccagaagttc aacaactgcg gcccgacgaa cctgaccctg
     1021 gtgctcaacc actacggcat cgacgtggat cagttcgccg tggccgcgct ggtgcgaccg
     1081 aattacgagg atcgcaacgt ctcgccggac gagttggtgg attatgtcaa tgagcagaca
     1141 cccctgcggg cggcggccta cgccggcggc gacatcgaca cgctgcgggc gctgctggcc
     1201 gccggcgtgc cggtcatcat cgagacgggg ctggagccgg acgcggccac gggctggatg
     1261 ggccactacc tgaccgtctt cgcctacgac gacatcaccc gccacttcag cgtccgcgac
     1321 acctacctgg ggccgtggca gggggagggg ctggccggct acgacgagac ggcgcggacg
     1381 tgggcgcagt tcaatggcgc gttcgtcgtc gtctatccgc cggagcgggc cgccgaggtg
     1441 gccgcggcgc tggggccggc cttcgccgac gcggcgacga tgtgggccgc ggccgtggac
     1501 gcggcgcgcg cggcagcgcg ggcgcggccc gacgacccct ttgcctggtt caacctgggc
     1561 agcggcctga ccgcgctggc cgggccgggg gacgcggcgt tgatggccgg ggcggcggcg
     1621 gccttcgacc gggcgcggca gctgggcctg ccgccgcgga tgctgtggta ccagtttgcg
     1681 ccctacgaag cctacctggc cgcgggccgc gccggcgacg tgctgacgct gacggcggtg
     1741 acactggaga atcagggcgg ccgcaacgtg gaggagacgt acctctatca gggcctcgcc
     1801 ctgctggcca cgggcgacgc cgccggggcg cgggcggcgc tggcgcgggc agtggaattg
     1861 aacgcggcct cgcccacggg caaggccgcg caagcggcgc tggacgggtt gtagcacagg
     1921 tcgttgacct gtgttccgca gagacaggcc aacggcctgt gctacggggg acaggccaac
     1981 ggcctgtgtt acaggggaca ggccaacggc ctgggctacg gggacaggcc aacggcctgt
     2041 gctacggctg cgcttgttcc atttcctcgc ggtgctcgat gagccagatg taggcgtcgg
     2101 tttcggtgcg gccggggaag tcggccagca ggttctgctc gcggatgtcg cggatggtgg
     2161 gcaggtagac gttatcgtac cacgaggcga cggcctcact gtagggcacg tggcgctgtt
     2221 gctccagccc caggtagtag tggtggacgt cgatgtggtg gaggatcttc tcgtacttgc
     2281 cgggcaggct ggccagcacg cgcgcgtccg gccgcagggc cagcagcccg gtgcgctcca
     2341 ggaaggcggc gtactccttg tcgcggatca tcgtatcgat ccagttcgcg ccgcgcagcg
     2401 tgcggatgac gcggcggacg acgccctgct ggtagatgcc ggccatgcgc tcggtggcct
     2461 cgtcgcgggc ctcgtcgctg cccacgtcgg cctgggccgc ctcgcgcacg agccactggt
     2521 actcgctgac acgcagatag aggtcggcgg cggtcatctc cgggaattgg cggcgcagcc
     2581 cctgggcctc gatggcatcg accagcggcc ggtagacgtt gtcgatccac gactgcacgg
     2641 cctcgccgta gctgacgtcg gcctggcgct ccgtgcccag gtagtagtgg tgggcggcga
     2701 tatgctcgcg caggcgggcg tattcggccg ggttgctcag gtgcaggtcg ctgtcggggc
     2761 gcaactcgtc caggcgggtg tcctgcacga actggccata ggccttctcc tgctgcacgc
     2821 tgtcgatgtc catgtcggcc gtcaggcgga aggggatgtc gatctcggtc acgaaggcgt
     2881 cgatgatggc ctgcttgcgc tcgcgggcca cggagacgcg gtggttgccg tccttgacga
     2941 agtaaacctc gccgatctta tacagctcga cgggcggcag ggcgacctcg ttgtagcggg
     3001 ccttgctgac attgacccag cgctcggtcg tctggcgttg ggtgggcagg aaggcgcggt
     3061 cgaagtcgcg gtagcggccg acgctgccga cgatcttgtc cagcgccacc tcttgcaggc
     3121 cgatgtcgcg ctggccgcgg tagggcaggg cctggcgcac ctcgtcgaag ggcagcagct
     3181 ggttgctctt gccggtcagc cgcgtgatga gcttgcgcca cagggagcgc tggtaggcgc
     3241 gttcaaagtc gtcgcgggcg agtgaatcgt acataggcgc gacgggaaac ggcggggcag
     3301 cctcccgatt cagcccattc tacgccgaac cgggcggc
//
LOCUS       DAOSMQ010000358         3327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28744_length_3327_cov_5.032702, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000358 DAOSMQ010000000
VERSION     DAOSMQ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28744_length_3327_cov_5.032702"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="PK829_11170"
     CDS             <1..1559
                     /locus_tag="PK829_11170"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU41815.1"
                     /translation="GAGGDRVDEFADLIAGHYAAAGDAAAEAAWQARAGAHAAARHAL
                     PEAERALSRALTLLESGAILPNRPPESGAILPNRPATAEDAIRENRATRFDLLLEREA
                     IYHIQGRRELQAADLDALDALAAALGDGDRLAEAALCRGFYGNATGDLVMAMTAAEAA
                     ERHAAPDDFTRRAKAHLIMAHAYRAQGVYDRARGLLETALALTRRGGPRAVEAEVLRS
                     LGMVAQEQGDIVGLRGYFEQALALARQSGDRRSERRTLNSMGVMEENEGNYEAAWAYF
                     EESLALARNLGDRTGTGTVLGNMGVTAMDTGDLATARTSFEEALAITRETDDAIGINI
                     WLLNLAFVVATEGDTERALALYEEARQGVEAAGDRPTLGYVLNGIGRVLLEAGRAGEA
                     LPWLRDALALREELAQAHLLSESQLYLAEALAVLGRTSEAMSYAGAALPRLLAEGLET
                     HEDTQRGLWAAYQALAAAGDARANEVLARLRAEIEAVAARLPIEMRPRYRNQFPWKRT
                     AIALSVPGTF"
     gene            1668..>3327
                     /locus_tag="PK829_11175"
     CDS             1668..>3327
                     /locus_tag="PK829_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU41816.1"
                     /translation="MSTPTIAPYGSWKSPITADLVLAGAVNLSQPRFDGDDIYWLEGR
                     ALERGRQVVVRRAPDGHTEDITPPAFNARTRVHEYGGGDYAVYEGVVYFANFDDQRLY
                     RQRPGEAPQPLTPAAGARYADLQVDAARGRLLAVREDHRGPGEAVNTLVAVSLHDGAT
                     TVLAEGNDFYSTPRLSPDGRKLAWLTWNHPNMPWDGTELWLADVATDGALGHARRVAG
                     GPAESIFQPAWSPPGADGDSDLIFTSDRSDWWNFYRLTGDGAGQPDEPLYPMAAEFGQ
                     PLWVFGMSTYAFVAPDRLVCSYTRNGRRAMALLDMGSGDLRPLDLALPGTMLAGSSGR
                     VVYVGVSPAEAAAVVLLEVSGDEVATTVLRRSASFSVDEDYLSIAEPVEFPTRGAILP
                     NRAAEDENSAIPENRATVAYGFFYAPRNRDYTAPVGELPPLIVTSHGGPTSATTDDLN
                     LSRQYWTSRGFAILDVNYGGSTGYGRAYRQRLNGNWGIVDVEDCINGARWLVEQGWVD
                     GERLIIRGGSAGGYTTLRAITAYHVFQAAASYYGISDAEALDRDT"
BASE COUNT          506 a         1204 c         1145 g          472 t
ORIGIN      
        1 tcggcgcggg cggcgaccgc gtggacgagt tcgccgatct catcgccggc cactatgccg
       61 ccgccggcga cgccgccgcc gaggccgcgt ggcaggcgcg ggccggggcg cacgccgccg
      121 cccgccacgc tctgccggag gcggaacgcg ctctcagccg cgccctgact ctgctggaga
      181 gtggcgcgat tcttccgaat cgcccgcccg agagtggcgc gattctcccg aatcgcccgg
      241 cgacggcgga agacgcgatt cgggagaatc gcgccacacg gttcgacttg ctgctggagc
      301 gcgaggccat ctaccacatc cagggccggc gcgagttaca ggccgctgac ctggacgcgc
      361 tcgacgcgct ggccgcggcc ctgggcgacg gcgaccggct ggccgaggcg gccctgtgcc
      421 ggggcttcta cggcaacgcg accggcgatc tggtcatggc catgactgct gccgaagcgg
      481 ccgagcgcca cgccgcgccc gacgacttca cccggcgggc gaaggcgcac cttatcatgg
      541 cccacgccta tcgcgctcag ggggtctatg accgggcccg tgggctgctg gagacggccc
      601 tggcgctgac ccggcgtggc ggcccgcgcg ccgtcgaggc ggaagtactg cgctcgctgg
      661 gcatggtcgc ccaggagcag ggcgacatcg tcggcttgcg cggctacttc gagcaggccc
      721 tcgccctggc gcgccagtcg ggcgaccggc gcagcgaacg gcgcacgctc aacagcatgg
      781 gcgtgatgga agagaacgag ggtaattacg aagccgcgtg ggcttatttc gaggaatccc
      841 tggcactggc ccgaaacctc ggcgaccgca ccggcaccgg taccgtcctg ggcaatatgg
      901 gcgtgacggc catggacacc ggcgacctgg ccacggcgcg gacgagcttc gaggaagctc
      961 tggccatcac ccgcgagacg gatgatgcca tcggcatcaa catctggctg ctcaacctgg
     1021 cttttgtcgt ggccacagag ggggacacgg agcgcgctct ggccctctat gaggaggccc
     1081 ggcagggggt tgaggccgct ggcgaccggc cgacgctggg ctacgtgctc aacggcattg
     1141 gccgcgtgct gctggaggcc gggcgcgccg gcgaggccct gccctggctg cgcgacgcgc
     1201 tggcgctgcg cgaagaattg gcccaggcgc atctgctgtc ggaatcgcag ctctatctgg
     1261 ccgaggcgct ggctgtgctg ggccgcacga gcgaggccat gagctacgcc ggggcggcgc
     1321 tgccccggct gctggccgag ggattggaga cgcacgagga cacccagcgc ggcctgtggg
     1381 ccgcctatca ggcgttggcc gccgccggcg acgcgcgggc caacgaggtg ctggcccggc
     1441 tgcgcgccga gatcgaggcc gtggccgccc gcctgccgat tgagatgcgg ccgcgctacc
     1501 gcaaccagtt cccctggaaa cggacggcga ttgccctctc cgtgcctggc actttctgaa
     1561 gtgcctggca cgttcttgaa cgcccccaga aggtgcgtcg cactcaagaa agtgcgacgc
     1621 accgaccccg gctggccatc ctaccccgcc agggctatca ttggcccatg tctacgccca
     1681 caatcgcccc ctatggctcc tggaaatcgc ccatcaccgc cgacctggtg ctggccggcg
     1741 cggtcaacct gtcgcagcct cgcttcgacg gcgatgatat ctactggcta gagggccggg
     1801 cgctggagcg cggccggcag gtcgtcgtcc gccgcgcgcc cgacggccac accgaggaca
     1861 tcaccccgcc ggcgttcaac gcccgcacgc gcgtccacga atacggcggc ggcgactacg
     1921 ccgtctatga gggcgtcgtc tattttgcta acttcgacga ccagcgcctc tatcgccagc
     1981 ggccgggcga agcgccgcaa ccgctgacac cggcggccgg cgcgcgctac gccgacctac
     2041 aggtcgatgc cgcccgcgga cgcctactgg ccgtgcgcga agaccatcgc ggcccgggcg
     2101 aggcagtcaa cacgcttgtg gccgtctcgc tccacgacgg cgcgacgacc gtgctggccg
     2161 agggcaacga cttctacagc acgccgcgcc tcagccccga cggccggaag ctggcctggc
     2221 tgacgtggaa ccaccccaac atgccctggg acggcacgga actctggctg gccgacgtgg
     2281 ccaccgacgg agcactgggc cacgcccgcc gcgtcgccgg cggcccggcc gaatccatct
     2341 tccagcccgc ctggtcgccg cccggcgcgg acggcgatag cgacctgatc ttcacttccg
     2401 accgcagcga ctggtggaac ttctatcgcc tgaccggcga cggggccggc cagcccgacg
     2461 aaccgctcta cccgatggca gccgagttcg gccaaccgct gtgggtcttt ggcatgtcca
     2521 cctacgcctt cgtcgctccc gaccggctgg tgtgcagcta cacgcgcaac ggccgccgcg
     2581 ccatggccct gctggacatg ggcagcggcg atttgcgccc gctcgacctg gccctgccgg
     2641 ggacgatgct cgccggcagc agcggccggg tcgtctacgt gggcgtctcg ccggccgaag
     2701 ctgccgccgt cgtcctgctg gaggtgagcg gcgacgaggt ggccacgact gtcctgcgcc
     2761 gctcggccag cttcagcgtc gatgaggact atctgtccat cgccgagccg gtcgagttcc
     2821 ccacccgtgg cgcgattctc ccgaatcgcg cggctgagga tgaaaacagc gcgattccgg
     2881 agaatcgcgc cacagtcgcc tatggcttct tctacgcgcc gcgcaaccgc gactacaccg
     2941 ctccggtggg cgagttgcca ccgctgatcg tcaccagcca cggcggcccg acctcggcca
     3001 cgaccgacga cctgaatctg agccgccagt actggacgag ccgcggcttc gccattctcg
     3061 acgtcaacta cggcggcagc accggctacg gccgcgccta ccgccagcgg ctgaacggca
     3121 actggggcat cgtcgatgtg gaggactgta tcaacggcgc gcgctggctg gtggaacagg
     3181 ggtgggtgga cggcgagcgg ctgatcatcc gcggcggcag cgccggcggc tacaccactc
     3241 tgcgggccat caccgcctat cacgttttcc aggccgcggc cagctactac ggcattagcg
     3301 acgccgaggc cctggacaga gacacgc
//
LOCUS       DAOSMQ010000359         3326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28753_length_3326_cov_4.058086, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000359 DAOSMQ010000000
VERSION     DAOSMQ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28753_length_3326_cov_4.058086"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK829_11180"
     CDS             complement(<1..523)
                     /locus_tag="PK829_11180"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOU41817.1"
                     /translation="MRLGKDFIDKPIYTLDEGKLLGKVQDLYLDDSLETVLGVYLGAQ
                     GLVRRRAQLIRSGDVTVFGVDAVLVKTAGVVTDDTELAAAKNWLRRDKVAGRDVDTPG
                     GTRLGVLGDVVIDATGRVTAFALSRVFVEGPVAEKRVIDRRAVQDTGSVDGRMTVDLS
                     RVEAALVDMPALPP"
     gene            complement(646..1926)
                     /gene="fabF"
                     /locus_tag="PK829_11185"
     CDS             complement(646..1926)
                     /gene="fabF"
                     /locus_tag="PK829_11185"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU41818.1"
                     /translation="MNSDFPRTAPPESQRAAAGSRVVVTGLGTLNPLGHDAAATWAAV
                     RAGRSGVGPITLFDAAGYETRIAAEVKNFDPVALFGRKEARRMARLTQLALAAAGQAL
                     ADAALGQALDPARVGVVVGSGMGALDPIAEAAETLATHGPARLSPFFVPMMLADTPAA
                     IISIVHGLTGPNLAVYSACASGNSALGEAAAMIRRGAADLMLAGGAEACVLPLALAGF
                     GVMGAISRRNDAPEQASRPFDAGRDGFVVAEGAAVLALEAREHALARGAHIYGELLGY
                     GSSADAYHITTPHETGAGAAASMAAALADAGLSPADVDYINAHGTSTAHNDRAETAAI
                     KRVFGERAYALPISSTKSMTGHLLGAAGALEAIFCLLALRDGLIPPTINYEQPDPACD
                     LDCVPNVARPAGLRVVMSNAFGLGGHNATLLFGR"
     gene            complement(1997..3172)
                     /locus_tag="PK829_11190"
     CDS             complement(1997..3172)
                     /locus_tag="PK829_11190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HOU41819.1"
                     /translation="MGSTATQATNARQNGTAVTEVPLSSADFHFAFHPNPDRIFDRAQ
                     RPFRLMRGKPSIKTYTNAIYAPAFRTAKQKLGCLYDESGNRIEASITRRNRSDMVYNT
                     DPLRFEGNAKELPVYDRPVLYLGFLYSHFSHFMIETLGHWWGLTEELGGIDRYLIHIS
                     DTSYLERSYVKAAMDALGITRDQLVFFDQPTRLSRVVVPANSVQIHSHIYTSYKDIFN
                     KLSLALGAADVAPTERAVFLSRSKDKHGVRRYHGEEEIEAFLAERGVTIAHPGQLPFR
                     EQVKLINEHSRIIGFQGGQMLNIAFALQPKEVVYLTDNRVWGSSLLTDICFDHSSTYV
                     KVADTENVLREMYRAVIRRTTGKRVKTGGFSRGHTVDLDRTTAWLAQMLASDRAPAR"
BASE COUNT          542 a         1045 c         1166 g          573 t
ORIGIN      
        1 ccggcggcag ggcgggcata tcgaccaggg ccgcctccac gcgggacaga tcgacggtca
       61 tgcggccgtc gacgctgccg gtgtcctgca cggcgcggcg gtcgatcacg cgcttctccg
      121 ccaccggccc ctcgacgaag acgcggctga gggcgaaggc agtgacgcgg cccgtggcgt
      181 cgatgacgac gtcgcccagg acgccgaggc gcgtgccgcc gggcgtgtcc acgtcgcggc
      241 cggcaacctt gtcgcgccgc agccagttct tcgccgccgc aagctcggtg tcgtcggtga
      301 cgacgccggc agtcttgacc agcaccgcgt cgacgccaaa gacggtcacg tcgccgctgc
      361 ggatgagctg cgcccgccgc cgcaccaatc cctgcgcgcc cagataaacg cccagtaccg
      421 tttccagact atcgtccaga tagaggtctt gaaccttgcc cagtagtttg ccctcgtcga
      481 gggtgtagat gggtttgtcg ataaaatctt tgcctagtct catgacgggt ctccttagcc
      541 agattgtaag aagctcacac caagaacgca acgatagggg ccaggttcca ggggccaggg
      601 gaagagcgct ccttccccta atctctaatc cctatcccct atctctcacc gcccgaagag
      661 taaggtcgcg ttgtgcccgc ccaagccaaa agcgttagac atgaccacgc gcagcccggc
      721 cgggcgggca acgttgggca cacagtcgag gtcgcaggcg gggtcgggct gctcgtagtt
      781 gatcgtcggc gggatcagcc cgtcgcgtag cgccagcaag cagaagatcg cttccagcgc
      841 cccggccgcg cccagcaggt ggccggtcat cgacttggtg gagctaatcg gcagggcgta
      901 ggcgcgctcg ccgaagacgc gcttgatggc cgccgtctcc gcccggtcat tgtgcgccgt
      961 ggaagtgccg tgggcgttga tatagtccac gtcggccggc gacagccccg cgtcggccag
     1021 cgccgcggcc atggatgccg ccgcccccgc gccggtttcg tgtggtgtgg tgatatggta
     1081 ggcgtcggcg ctgctgccat agcccagcaa ctcgccatag atgtgcgcgc cacgggccag
     1141 ggcgtgctca cgcgcctcca gcgccagcac ggccgcgccc tcagcgacaa cgaagccatc
     1201 gcgcccggca tcaaacggcc gcgaggcttg ctctggcgcg tcgttgcgcc ggctgatggc
     1261 tcccatgacc ccgaagccgg ccagcgccag cggcaagacg cacgcctctg ccccgccggc
     1321 cagcatgagg tcggccgcgc cgcgccggat catggccgcg gcctcgccga gggcgctgtt
     1381 gccgctggcg caggcggagt agacagccag gttcggcccg gtcaggccgt ggacgataga
     1441 gatgatcgcc gccggcgtgt cggccagcat cataggcacg aagaaggggc taaggcgggc
     1501 cggcccgtgg gtggccaacg tctcagccgc ctcggcaata gggtctagcg cgcccatgcc
     1561 gctaccgacg acgacgccca cccgcgccgg gtcgagcgcc tggcccagcg ccgcgtcggc
     1621 cagcgcctgc ccggccgcgg ccagagccag ttgtgtcagc cgggccatgc ggcgggcctc
     1681 tttgcggccg aagagggcca cggggtcgaa gttttttacc tcagcggcga tgcgcgtttc
     1741 gtagccagcg gcgtcgaaga gagtgatggg gccgacgcca gagcggcccg cgcgaaccgc
     1801 cgcccacgtc gcggcggcgt cgtggcccaa cgggttgagc gtgcccaggc cagtgacgac
     1861 gacgcggctg ccggccgcgg cgcgctggct ttccggtgga gccgtgcggg ggaaatcgct
     1921 gttcatcgtc gtgaagtata acacgacgat gaacagcgtg gtggagtgga ggggagtggg
     1981 ttggatcgcc ggatggttaa cgcgccggcg cgcggtcgct ggcgagcatt tgcgccaacc
     2041 aggccgtagt ccggtccaga tcgaccgtgt ggccgcgcga aaagccgccg gtcttgaccc
     2101 gcttgccggt tgtccgccgg atgacggcgc ggtacatctc gcgcaagacg ttctcggtgt
     2161 cggcgacctt gacataggtg gaggagtggt cgaagcaaat atcagttagc agcgacgacc
     2221 cccagacgcg gttgtcagtc aggtagacaa cttctttcgg ctggagggcg aaggcgatat
     2281 tgagcatctg cccgccctgg aagccgatga tgcggctgtg ttcgttgatc agcttgacct
     2341 gctcgcgaaa cggcagctgg ccggggtggg caatggtgac gccccgttcg gccaggaagg
     2401 cctcgatctc ctcttcgccg tggtagcgcc ggacgccgtg cttatccttc gaccgcgaca
     2461 ggaacaccgc ccgttcggtg ggggcgacgt cggccgcgcc cagcgccagc gagagcttgt
     2521 tgaagatatc cttgtagctg gtgtagatgt ggctgtgaat ctggacggag ttggccggca
     2581 caacgacccg gctgaggcgt gtcggctggt cgaagaagac gagctggtcg cgagtgatgc
     2641 ccagggcgtc catggccgcc ttgacgtagg agcgctccag atagctggtg tcgctgatgt
     2701 ggatgagata gcgatcgatg ccgcccagtt cctccgttag cccccaccag tggcccagcg
     2761 tctcgatcat gaaatggctg aagtgggagt agagaaagcc cagatagagc accgggcggt
     2821 cgtagacggg cagctctttg gcgttgccct cgaagcgcag cgggtcggtg ttgtagacca
     2881 tatcgctgcg gttgcgccgc gtgatcgacg cctcaatgcg attgccgctc tcgtcataga
     2941 gacaaccgag cttctgcttg gcggtgcgga aggccggggc gtagatggcg ttggtgtagg
     3001 tcttaatcga cggcttgccg cgcatcaggc ggaaagggcg ctgggcgcgg tcgaagatgc
     3061 ggtcggggtt ggggtggaag gcaaagtgaa aatcggcgct ggagagcggg acttcggtga
     3121 cggcagtccc gttctggcgc gcgtttgtgg cttgtgttgc tgtgctgccc atgtgtggtt
     3181 ctgacctctc attgcggtgt cgtctcccat tgtgtcggtt ttaccttacg ggttgcatta
     3241 agtgaaaggc ggtctcgtag cacagcctgt caggctgtcc gtccgaacgc ggcccagggc
     3301 ctggtaaacg gtcttcgcgc ccagcc
//
LOCUS       DAOSMQ010000360         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28837_length_3318_cov_3.683114, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000360 DAOSMQ010000000
VERSION     DAOSMQ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28837_length_3318_cov_3.683114"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1050)
                     /locus_tag="PK829_11195"
     CDS             complement(121..1050)
                     /locus_tag="PK829_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOU41820.1"
                     /translation="MKHPTLLALVLLVLLASACAGGAAASSDDGHLTVVTTTGQIGDT
                     ARIVGGDHVRVLSLMGPGTDPHLYVAGISDVDKLRGADIVFYNGLFLEAQMEAVLEQL
                     AERQTVVAVSAGIDPAKLLPSAKFANEFDPHIWFDVRLWMQTVEQVRDTLMAADPANA
                     ADYEANAAAYLAELTALDAYVTAQAARVPEAQRILITAHDAFNYFGRAYGFEVLGLQG
                     ISTASEASTADVQRLADFIATARVPAVFIESSVPVRNVEAVQAAVRDRGFDVRIGGQL
                     FSDAMGDTDTPEGTYVGMVRYNIDTIVGALLAK"
     gene            complement(1047..1811)
                     /locus_tag="PK829_11200"
     CDS             complement(1047..1811)
                     /locus_tag="PK829_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOU41821.1"
                     /translation="MTNLVAANLSVRQGAGRPSHPGPAAGDYLRALFALGENGRAVTT
                     TEVAGRVGVRPASATAMLQKLATAEPPLVDYHKNRGARLTAAGERAALAIVRCHRLLE
                     LFLHEKLGYGWDEVHDEADRLEHVISADLADRLAAALGQPTHDPHGHAIPAADLSLAI
                     PAALPLSALTPGQSATVLHVSDDNAAALRRLASFNLRPGAIVTREEAAGDGAVRLRLR
                     AGALIAVSPADAARVFVAPPRPEDIDYTQSGDFHTP"
     gene            complement(1968..2414)
                     /locus_tag="PK829_11205"
     CDS             complement(1968..2414)
                     /locus_tag="PK829_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOU41822.1"
                     /translation="MRHGLQGLPVDDWTTAGVCGWWSVREIIAHLASFEVMLLDVLAI
                     AAGETDIHGSTLRAWARDGQRFNDEQVAARAALSPEETLADYIAAHEAVMARIAALPD
                     GATTRAGFLPTYGREYDLDDFLTYSFYGHKREHTAQIAVFRDAIKR"
     gene            complement(2538..2912)
                     /locus_tag="PK829_11210"
     CDS             complement(2538..2912)
                     /locus_tag="PK829_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOU41823.1"
                     /translation="MQPKEVTRLYLARHNAHDVEGVMALFAENACYEIPGRLVRHGKG
                     EIRQAEEWEAAVHDQIRTGDLVQADHEVSFRAFASNEWLRAAGVDELRYTSMTIAVRA
                     GHIIRITAALAPESLRAIETAH"
BASE COUNT          595 a         1018 c         1145 g          560 t
ORIGIN      
        1 gcatcttctt gagcggcgtg tccagaaagt gccaggcact gtcgagtcgg tcggtgcgtc
       61 gcaccttctt gagcggcatg tccagaaagt gccaggcact ccagaaagtg ccaggcactg
      121 ctatttggcc aggagggcgc cgacaatcgt atcgatgtta tagcgcacca tgcccacata
      181 cgtcccttcc ggggtgtccg tgtcgcccat ggcgtcggag aagagctgcc cgccaatgcg
      241 cacgtcgaag ccgcggtcgc gcacggcggc ctgcacggcc tccacattgc gcacgggcac
      301 ggacgactcg atgaagacag ccggaacgcg ggccgtggcg atgaagtcgg ccaggcgctg
      361 cacgtcggcc gtgctcgcct ccgaggcggt gctgatccct tgcagaccaa gcacctcgaa
      421 gccataggcg cggccgaagt agttgaaggc gtcgtgggcc gtgataagga tgcgctgggc
      481 ttcgggcacg cgggcggcct gggcggtcac gtaggcgtcc aacgcggtta gctcggccag
      541 ataggcggcg gcgttggcct cgtagtcggc ggcattggcc gggtcggcgg ccatgagcgt
      601 gtcgcgcact tgctcgaccg tctgcatcca caggcgcacg tcgaaccaga tgtggggatc
      661 gaactcgttg gcgaacttgg ccgagggcag cagtttggcc gggtcgatgc cggccgagac
      721 ggcgacgacg gtctgccgtt cggccaactg ttccagcacg gcctccattt gcgcttccag
      781 gaagaggccg ttgtagaaaa cgatgtcggc gccgcgcagc ttgtcgacgt cgctgatgcc
      841 ggcgacgtag aggtgggggt cagtgcccgg ccccatcagg ctgagcacgc ggacgtggtc
      901 gccgccaacg atgcgggccg tatcgccgat ctggccggtg gtggtgacga cggtcaggtg
      961 gccatcgtcg ctgctggccg ccgcgccgcc ggcgcaggcc gacgccagca agacaagcaa
     1021 gaccagggcg aggagggtag ggtgtttcat ggcgtgtgga agtctccaga ttgcgtgtag
     1081 tcaatatctt ctggtcttgg tggggcgacg aaaacgcgcg cggcatcggc cgggctgacg
     1141 gcaatcaacg ccccggcgcg cagacgtagc cgcacagcgc cgtcgccggc agcctcctcc
     1201 cgcgtcacga tcgcgcccgg tcgcagattg aacgaggcca agcgccgcag cgccgcggcg
     1261 ttgtcatcac tgacgtggag gacagtggcc gactggcccg gcgttaacgc gctcaggggg
     1321 agggcggccg ggatggccag tgacagatcg gccgcgggaa tggcgtggcc gtgggggtcg
     1381 tgcgtgggct ggccgagggc cgcggccaag cggtcggcca aatcggcgct gatgacgtgc
     1441 tccaggcggt cggcctcgtc gtggacttcg tcccagccgt agccgagttt ttcgtgtagg
     1501 aacagctcta gcagccggtg gcagcgcact atagccaacg cggcgcgttc gccggcggcc
     1561 gtcaggcgcg cgccgcggtt tttgtgatag tccaccagcg gcggctcggc cgtggccagc
     1621 ttctggagca tggccgtcgc cgacgccggg cggacgccga cgcgcccggc cacctcggtt
     1681 gtggtcacgg cccggccatt ctcgcccagg gcaaagaggg cgcgcagata gtcaccggcg
     1741 gcggggccag ggtgggaggg cctccccgcg ccctgcctaa cacttaaatt agccgcgact
     1801 aaattagtca tggctaaagt atacgcctgg cgcgttgtaa gtcaagggag aattcagcca
     1861 acagccacct gacaggtggc gaccctgtct ccccggtagc cgggagattg tggccgtcca
     1921 ccggtcaagt cgatgaaaag cggcgcggcc ccagccacgc cggcccttca gcgcttaatg
     1981 gcgtcgcgga agacggcgat ctgggcagtg tgctcgcgct tgtggccgta gaacgaatag
     2041 gtcaggaagt cgtcaaggtc atactcgcgg ccgtaggtgg gcaggaagcc ggcgcgcgtt
     2101 gtcgctccgt ctggcagagc ggcgatgcgc gccatcaccg cctcatgcgc ggcgatatag
     2161 tccgccagcg tttcttccgg cgacagcgcg gcgcgagcgg caacctgctc gtcgttaaac
     2221 cgctgaccgt cgcgcgccca ggcgcgaagg gtggagccgt ggatgtctgt ctcgccagcg
     2281 gcaatggcca gcacgtcgag cagcatgacc tcgaacgagg ccaggtgggc gatgatctcg
     2341 cgcaccgacc accagccgca gacgccggcc gtcgtccagt cgtctacggg cagaccctgg
     2401 agaccgtgtc gcacagttag attgccgtac ttcagaacat cagcagcatg catatgcttc
     2461 gcctctctgc gaaattggcc ggcgcggcgc gccggccgtg ccagagaggg aagcatacca
     2521 caaacggtcg cgcgccgtca gtgcgcggtc tcgatggcgc gcaacgactc cggcgcaaga
     2581 gcggcggtga tgcggatgat atgaccggcg cgcacggcaa tggtcattga ggtataccgc
     2641 agttcatcga cccccgcggc gcgcaaccac tcgttgctgg cgaaggcgcg gaagctgacc
     2701 tcatggtcgg cctggaccag gtcgccggtg cggatctggt cgtggacggc cgcctcccac
     2761 tcttcggcct gacggatttc acccttgccg tggcgaacaa gacggcccgg aatttcgtag
     2821 caggcgttct cggcaaagag agccatgacg ccttccacgt catgggcatt gtgccgggcg
     2881 aggtacaagc gagtaacttc cttaggttgc attcagtccc acctccacca acaatgatag
     2941 cgggccatga cgcgccgtgt caataagcga ttggtcatgg tggtaggaac tcacgcaaag
     3001 tcgccaagag gccaaggcgc aaaggaagaa aagaagatct cgcgcaaaga cgcagagtac
     3061 gccgagctta agaggctttc ttaactttgt gtacttcgcg tctttgcgtg agatttgcgt
     3121 gagccttgcg gcgccctgct cttttcggcg cggctggcca ccagcgcgaa ggctgctaca
     3181 atacccgccc actagtaaca aaagttggag aggcgcatga aactatcgga attcctggaa
     3241 gattacggcg gctggctggc attcatcatt gccctggtgg cgacgttggg cagcctgtac
     3301 tacagtgaga tcgccggg
//
LOCUS       DAOSMQ010000361         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28849_length_3317_cov_3.812692, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000361 DAOSMQ010000000
VERSION     DAOSMQ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28849_length_3317_cov_3.812692"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2310
                     /locus_tag="PK829_11215"
     CDS             <1..2310
                     /locus_tag="PK829_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU41824.1"
                     /translation="LRRYRPRHGWLPFGLLLATLACLALSVLDVGWTPEDGVVVPVLA
                     LGFLLAARLAQRGVRLLPAGAMLVVAGAALALVAGLWPPGAIARGGAAELLAFWTRQL
                     ALFADRAAGWARAVAAGGRSTETVAFTLGLALAGWFVAATLAWCVYRLRRPTWGLSLV
                     GAALAVNTFYGQAGLYWAVIFFGLAITAATYLDYLFREDDWDAAGVDYSSEVRPDLLI
                     YAAGISLGLMSLAMALPAINFRAIAEAFQRQDAIVATEQTLERAFAGVQQPRTDEGAA
                     GAGGLPRAFLLGGDPKLSETVVMTATVALPAGLSPGTLLSARHWRSVSYDEYTGRGWR
                     RSSEREEAVVAGQALPEGGDVPARRLVPLRETVAWLYDRRATRYTVGRPAVFSHDLTV
                     YWRGAADLVGVSGRNLAPHRYSVEATAALLDSGLAAARLEDVPPEVLARYTALPNDVP
                     ARVHALARQAAGLDATTGVTPPSPYEQARAIETFLHQYPYTLDLPPPPADVDIVDYFL
                     FDLQKGFCDYYASAMVVMARSVGLPARLAAGFRQQPADAAGVQTVRQINAHSWAEVYF
                     AGYGWVEFEPTAAFAAATAPAAPAAAGQPAPAFEPPAPVAGLPPRAPRRETPWAALLG
                     LAALAVVVWQLWGRRLAADARRPRASLDPVQAAFAGLQDSAAAAGFPYRDGQTPAEFA
                     AALLARLGEGADDSHGAVERLAQLFAARQYGAGAAAGPGAASAAAGEAHAAWQAARRP
                     LRRLAWRRRLGRRAHETSPSSTDGPAEVR"
     gene            2355..>3317
                     /locus_tag="PK829_11220"
     CDS             2355..>3317
                     /locus_tag="PK829_11220"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOU41825.1"
                     /translation="MKQDDFDIPEVFRRAMEEAGWRENEEGGGRRPPRRPLPPPMRSP
                     RFSRLLVLAIVILIFLFSLGSIARFYTDFLWFDHLGFRDMFVKRLTVRAIVFVIAFVV
                     AAAALVGNWLLARRRSLRETTPLQPGPLRGGVVRALLIGAGLAVAFLFASAAASQWEE
                     LLLWLNAGSFGVTEPIFGRDVSFYVFKLPVLEFFQGWIITLLFLTGLGLIVVYAGANL
                     LDIQRGTWRPLQSAAARRHLALLAGVLMLVWAAGYALDTYRLLFSDRGVAFGASYVDL
                     RANLWSLRLQAIFAVLTAVALLYNVFRLNVRLPVLAGALWILSGI"
BASE COUNT          356 a         1254 c         1159 g          548 t
ORIGIN      
        1 ctccggcgct accggccgcg ccacggctgg ctgccctttg gcctgctgct ggcgacgctg
       61 gcctgcctgg ccctgtcagt gctggacgtc ggctggacgc cggaggacgg cgtcgttgtg
      121 cccgtgctgg cgttgggctt cctgctggcg gcgcggctgg cccaacgggg tgtccggctg
      181 ctgcccgccg gggccatgct cgtggttgcc ggcgcggcgc tggcgctggt ggccggtcta
      241 tggccgcccg gggccatcgc ccgcggcggc gcggccgaac tgctcgcctt ctggacgcgg
      301 caactggccc tcttcgccga ccgcgccgcc ggctgggcgc gggccgtcgc cgccggcggc
      361 cgcagcaccg aaacggtcgc cttcactctg gggctggctc tggcgggctg gttcgtggcc
      421 gcgacactgg cctggtgcgt ctaccggctg cggcggccca cctgggggct gtcgctggtc
      481 ggcgcggccc tggccgtcaa caccttttat ggccaggcgg gcctctactg ggccgtcatc
      541 ttctttggcc tggccatcac tgcggccact tatctggact atctcttccg cgaggacgat
      601 tgggacgccg ccggcgtgga ctattccagc gaggtgcgcc ccgacctgct catctacgcc
      661 gcgggcatca gcctgggcct catgtcgctg gccatggccc tgccggcgat caacttccgg
      721 gccatcgccg aggccttcca gcggcaggat gccatcgtgg ccaccgagca gacgctggag
      781 cgggccttcg ccggcgtcca gcagccgcgc accgacgagg gcgcggccgg cgcgggcggg
      841 ctgccgcggg ccttcctgct gggtggcgac ccgaaactga gcgagacggt cgtcatgacc
      901 gccaccgtag ccctgccggc cggcctgtcg ccgggcaccc ttctctcggc ccgccactgg
      961 cgctcggtca gctacgacga gtacaccggc cgcggctggc ggcggtcgtc ggagcgcgag
     1021 gaggcggtcg tcgccgggca ggccctcccg gagggcggcg acgtgcccgc ccggcggctg
     1081 gtgcccctgc gcgagacggt ggcctggctc tacgaccgcc gggcgacgcg ctacaccgtc
     1141 ggccgcccgg ccgtcttcag ccatgacctg accgtctact ggcgcggcgc ggccgacctc
     1201 gttggcgtca gcgggcgcaa cctggccccg caccgctata gcgtcgaggc gacggcagcc
     1261 ctgctcgaca gcggcctggc cgccgcccgc ctggaagacg tgccgccgga ggttctcgcc
     1321 cgctatactg ccctgcccaa cgacgtgccc gcccgcgtcc acgccctggc ccgccaggcg
     1381 gccggactgg acgcgacgac gggcgtgacg ccaccctcgc cctacgagca ggcgcgcgcc
     1441 atcgagacat tcctgcacca gtacccctac acccttgacc tgccgccgcc gccggccgac
     1501 gtcgatatcg tcgattattt cctcttcgac ctgcaaaagg gcttctgcga ctactatgcc
     1561 tcggccatgg tcgtcatggc ccgctcggtg gggctgccgg cgcggctggc ggccggcttc
     1621 cggcaacagc cggccgatgc cgccggcgtc caaacggtgc gacaaatcaa cgcccactcc
     1681 tgggccgagg tctattttgc cggctacggc tgggtggagt ttgagccgac ggccgccttc
     1741 gccgcggcaa ccgcgccggc cgccccggcc gcggcgggcc agcccgcgcc ggccttcgag
     1801 ccgccggcgc cggtggctgg cctgccgccg cgcgctcccc ggcgcgagac accgtgggcg
     1861 gcgctgctgg ggctggcggc gctggcggtc gttgtgtggc agctctgggg gcggcggctg
     1921 gccgcggacg cccgccggcc gcgcgcctct ctcgaccccg tgcaggccgc cttcgccggg
     1981 ctgcaagaca gcgccgccgc ggccggcttc ccctatcgcg atgggcagac gccggccgag
     2041 ttcgccgccg ccctgctggc ccgcctgggc gagggcgcgg atgatagcca cggcgcggtg
     2101 gagcggctgg cccagctctt cgccgcgcgg cagtatggcg cgggcgctgc tgccggccct
     2161 ggggccgctt cggccgcggc cggcgaagcc catgccgcct ggcaggccgc ccggcggccg
     2221 ctgcgccggc tggcctggcg gcgtcgcctc ggccggcggg cacatgagac ctcgccatcg
     2281 tctacagacg gcccggccga agtgcgctag aatcatctcc ggtagctcag cccacgcaga
     2341 cagcgagtgg aactatgaaa caagatgact ttgacattcc ggaggtcttc cggcgcgcca
     2401 tggaagaggc cgggtggcgc gagaacgagg agggcggcgg ccggcgcccc ccacgtcgcc
     2461 ccttgccgcc gccgatgcgc tcgccgcgct tcagccgcct tctcgttctg gccatcgtca
     2521 tcctgatatt cctcttcagc ctgggcagca tcgcccgctt ctacaccgac ttcctctggt
     2581 tcgaccacct cggcttccgc gatatgtttg tcaagcggct gaccgtgcgc gccatcgtct
     2641 tcgtcatcgc cttcgtcgtc gccgcggccg cgctggtggg caactggctg ctggcccgcc
     2701 ggcgttcgct gcgcgagacg acgcccctgc aaccagggcc actgcgcggc ggcgtcgtgc
     2761 gcgcgctgct catcggcgcg ggtctggccg tggccttcct gttcgcctcg gccgccgcca
     2821 gccagtggga ggagctgctg ctgtggctca acgccggcag ctttggcgtg accgagccga
     2881 tcttcggccg cgacgtgagc ttctacgtct tcaaactgcc cgtgctggag ttcttccagg
     2941 gctggatcat caccctgctc ttcctgaccg gtctgggtct catcgtcgtc tacgccgggg
     3001 ccaacctgct cgacatccag cgcggcacgt ggcgaccgct gcaatcggcc gccgcccgcc
     3061 gccatctggc cctgctggcc ggcgtactca tgctcgtctg ggccgcgggt tacgcgctcg
     3121 atacctaccg gctgctcttt tccgaccgcg gcgtggcctt cggcgcgagt tatgtcgact
     3181 tgcgcgccaa cctgtggagc ctgcgcttgc aggccatctt cgctgtgctg acggccgtgg
     3241 ccctgctcta caacgtcttc cggctcaacg tccgcctgcc tgtcctggcg ggggcgttgt
     3301 ggattctcag cgggatc
//
LOCUS       DAOSMQ010000362         3312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28911_length_3312_cov_3.485109, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000362 DAOSMQ010000000
VERSION     DAOSMQ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28911_length_3312_cov_3.485109"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PK829_11225"
     CDS             <1..827
                     /locus_tag="PK829_11225"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOU41826.1"
                     /translation="RKLQAAHLSGQRTFSDFGKALPAVCHTPSCRLYSITEVRQMTIP
                     DATEIVAELERLLPDWKEMEPGRWQRVEYALRAAEGDGPVFPTFESFIEWVDEDTSAE
                     WVEGKVYFMSPASLRHQLIAGFLYKIIDAYVEHHRLGVTLNAPFKMKLPGYGAEPDVL
                     VVLNENSGRLRPTYLNGPADLVVEVISPDSVERDRVKKYGDYEAAGVNEYWLIDPEQR
                     TAEFYRLSGGRYAAVEPDADGWVASAVLPGFRLRAAWLFQNPLPKLLDVLADLKIL"
     gene            931..2070
                     /locus_tag="PK829_11230"
     CDS             931..2070
                     /locus_tag="PK829_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOU41827.1"
                     /translation="MPKKSNKVRVAIIGVGNCANALIQGVHYYRDAADDQEIPGLMHA
                     TVGGYHVRDIEFSAAFDVVTTKVGKDLSEAMWAYPNDTIKFADVPYLNVPVYRGMTHD
                     GIGKYLSQKVEKAPGPTDDIIKILKETKTDVVVNFMPVGSEMATKWYVEQIIEAGCAF
                     VNGIPVFIASSPYWGKRFEDAGLPIIGDDVKSQLGATITHRVLTTLFKDRGIHIDRTY
                     QLNFGGNMDFYNMLERERLESKKISKTNAVTSQLPYDLGADNVHVGPSDYVPWLTDRK
                     WCHIRMEGRAFGDVPLQLELKLEVWDSPNSAGVIIDAVRCAKLALDRGIGGPLTAPSS
                     YFMKSPPVQYTDDEARRRVQEFIAAQPAHTSQAAPEGNGQLAAAD"
     gene            2167..2631
                     /locus_tag="PK829_11235"
     CDS             2167..2631
                     /locus_tag="PK829_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOU41828.1"
                     /translation="MAARVQQWAAILSNQRGESRSREGGMLSDSQFEELVGEALDNLP
                     PFFQAQMDNIVVLVELWPSRRVLREMDVPPGETLLGLYRGVPLTERTHAYNLVTPDSI
                     TLFQGPIEAAAASWDEGDYFDRVREEVWHTVVHEIAHHFGIDDDRLHELGAY"
     gene            complement(2638..>3312)
                     /locus_tag="PK829_11240"
     CDS             complement(2638..>3312)
                     /locus_tag="PK829_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41829.1"
                     /translation="RRIAFQSDRAGNDDIYVMARDGSDQTRLTTSPDSDRQPGWSPDG
                     TQLVFFSRGNSGSDLYAIDAGGGNPRRLTATPLNEGHVSWSVDGRLVYNVTIEDRFQV
                     YTSTSMGTEEQRLTFTEGDDWSPEWSPDGRTMVFLSERDGTNRNPAIYVMNADGSGQR
                     SLYDSPDYEWSPSWSPDGRQILFTAASGAQANARDDIYLMNADGSGARLAAQSGSYPS
                     WAVAVP"
BASE COUNT          602 a         1086 c         1058 g          566 t
ORIGIN      
        1 aaaggaaact tcaggccgcc cacctgtcag gtcagcgcac ttttagcgac tttggaaaag
       61 ccctgccggc cgtttgccac accccatcct gccgcctata ctctatcacc gaggtgcgcc
      121 agatgaccat tcccgacgcg acagagatcg tggccgagtt ggagcggctg ttgccagact
      181 ggaaagagat ggagccgggc cgctggcagc gagtggagta tgctttgcgc gcggccgagg
      241 gcgacgggcc ggtcttcccc acgtttgagt cgttcatcga gtgggtggac gaagacacgt
      301 cggccgaatg ggtggagggg aaggtctact tcatgagtcc cgcatcgctg cgacatcaat
      361 tgatcgctgg tttcctgtat aagatcatcg acgcctatgt cgaacatcac cgcctgggcg
      421 tcacgctcaa cgcgccgttc aaaatgaagc tccccggtta tggcgctgag ccggacgtgt
      481 tggtcgtcct gaacgagaac agcggccgtc tgcggcccac ctatctcaac ggcccagccg
      541 acctcgttgt cgaagtcatt tcgcccgata gcgtcgagcg cgaccgggtg aagaagtatg
      601 gcgactatga ggccgccggc gtgaatgagt actggctcat cgacccggag cagcggacgg
      661 ccgagttcta tcggctgagc ggcgggcgct atgcggcggt cgaaccggac gcggacggct
      721 gggttgcgtc ggccgtgttg ccgggcttcc ggctgcgcgc cgcctggctg ttccagaacc
      781 cactgcccaa gctgctggat gtgctggccg atctgaaaat cctctaatcc cgcgcctttt
      841 tcggccaact tacgtataat cactgaaaga aacttgcggc gcggcgggcg cggttgccca
      901 cacgcgcgtc cttaacccca ggagatcacc atgcccaaga aaagcaacaa ggtgcgcgtc
      961 gccatcatcg gcgtcggcaa ctgcgcgaat gcgctcattc agggcgtcca ctattatcgg
     1021 gacgccgcgg acgaccagga aatccccggt ttgatgcatg ccaccgtcgg cggctaccac
     1081 gtccgcgata tcgagttctc agccgccttc gacgtcgtca ccaccaaggt cggcaaggac
     1141 ctcagcgagg cgatgtgggc ctatccgaac gacacgatca agttcgccga cgtgccctac
     1201 ctcaacgtgc ccgtctaccg cggcatgacc cacgacggca tcggcaagta tctcagccag
     1261 aaggtcgaga aggcccccgg cccgacggac gacatcatca aaatcctgaa ggagacgaag
     1321 accgacgtcg tcgtcaactt catgcccgtc ggttcggaga tggccacgaa gtggtacgtc
     1381 gagcagatca tcgaggccgg ttgcgccttc gtcaatggca tccccgtctt catcgccagc
     1441 tcgccctact ggggcaagcg cttcgaggac gccggcctgc ccatcatcgg tgacgatgtc
     1501 aagagccagc tcggcgcgac catcacccac cgcgtcctga cgaccctgtt caaggaccgc
     1561 ggtatccaca tcgaccgcac ctaccagctc aacttcggcg gcaacatgga cttctacaac
     1621 atgctggagc gcgagcggct ggagtcgaag aagatctcca agaccaacgc cgtcaccagc
     1681 cagctgccct atgacctggg cgcggacaac gtccacgttg ggccgagcga ctacgtgccc
     1741 tggctgaccg accgcaagtg gtgccacatc cgcatggaag ggcgcgcctt tggcgacgtg
     1801 ccgttgcaac tggagttgaa actggaagtg tgggactcgc ccaactcggc cggcgtcatc
     1861 atcgacgcgg tgcgctgcgc caagctggcc ctcgaccggg gcatcggcgg cccgctgacc
     1921 gcgccgtcgt cgtacttcat gaagtcgccg ccggtgcagt acaccgacga cgaagcccgg
     1981 cggcgggtgc aggagttcat cgccgcgcag ccggcccaca cctcccaggc cgcgccggag
     2041 ggcaacggcc agctggccgc ggccgactag cgaagccgtg gcgcgattct ccgaatcgcg
     2101 aagccgtggc gcgattctct gaatcgcgaa gccacagcaa actgcctgat atagtcatat
     2161 gcagggatgg cggcccgcgt tcagcagtgg gccgccatcc tctctaacca gaggggcgag
     2221 agccgctcgc gcgagggtgg gatgctaagc gatagccagt tcgaggagct tgtcggcgag
     2281 gcgctggaca atctgccgcc cttcttccag gcgcagatgg ataacatcgt cgtgctggtg
     2341 gaactgtggc cgtcgcggcg ggtgctgcgc gagatggacg tgccgccggg cgagacgctg
     2401 ctgggcctct accgcggcgt ccccctcacc gagcgcaccc acgcctacaa cctcgtcacc
     2461 ccggactcga ttaccctctt tcaaggcccc atcgaggccg ccgcggccag ttgggacgag
     2521 ggcgattact tcgaccgcgt gcgcgaagag gtctggcaca cggtcgtcca cgagatcgcc
     2581 caccacttcg gcatcgacga cgaccgcctg cacgaactgg gcgcatactg agcgacctta
     2641 gggcacggcc acggcccacg acgggtaaga gccggactgc gccgccagcc gcgccccgct
     2701 gccgtcggcg ttcatgaggt agatgtcgtc gcgcgcgttg gcctgcgcgc cgctggcggc
     2761 ggtgaagaga atttgccggc cgtcgggcga ccaactgggc gaccactcat aatccgggct
     2821 gtcgtagagc gaccgctgcc cgctgccgtc ggcgttcatg acgtagatag ccgggttgcg
     2881 gtttgtgccg tcgcgctccg acaggaagac cattgtccgc ccgtcgggcg accactccgg
     2941 cgaccagtcg tctccctctg tgaacgtcag ccgttgctcc tccgtcccca tgctggtgct
     3001 ggtgtacacc tggaagcgat cctcgatggt gacgttgtag accagccggc catcgaccga
     3061 ccaggacacg tgcccctcgt tgaggggcgt ggccgtcagc cggcgcgggt tgccaccgcc
     3121 ggcgtcgatg gcgtagaggt cgctgccgct gttgccgcgc gagaagaaga cgagctgcgt
     3181 gccgtcgggc gaccagcctg gctgccggtc gctgtcgggg ctggtggtca ggcgcgtctg
     3241 gtcgctgcca tcacgggcca tgacgtagat atcatcgttg ccggcgcggt cagactggaa
     3301 ggcgatgcgc cg
//
LOCUS       DAOSMQ010000363         3309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_28939_length_3309_cov_4.598033, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000363 DAOSMQ010000000
VERSION     DAOSMQ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28939_length_3309_cov_4.598033"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1164)
                     /locus_tag="PK829_11245"
     CDS             complement(121..1164)
                     /locus_tag="PK829_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOU41830.1"
                     /translation="MIKKASWQRNARAAWRDTLVLLRQFAWPLLLFVVVLVGGGLLYY
                     ALAAYAGQRLDGPIESIYGVLEMIFLQPLGEFPDVWYLQLFYFVMPIVGIGILAFGLA
                     DFGRLFFNRRARRKEWEMAVASTFSNHVILVGLGHLGFRVMQELVERGQDVVVIEQNP
                     KANLADEARRLRVPVIQDDGQRQETLIGAAVARARAIVLCTQNDVLNLQVAFMARRLN
                     PDIEVVMRIFDDAFAEEVARQFGYRAMSATGMAAPTFAAAATGMDIARPITIEGQPFS
                     LCSLTVGRGSRLDGLSVGAVERDYDVSVVLLRRDGHSDHHPAAERALAAGDVVAVLGG
                     PEPIGHIARANHL"
     gene            complement(1285..2376)
                     /gene="amrS"
                     /locus_tag="PK829_11250"
     CDS             complement(1285..2376)
                     /gene="amrS"
                     /locus_tag="PK829_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU41831.1"
                     /translation="MTPTISGTPTTHWHRLADGRVQCDVCPRACRLHDGQRGLCFVRG
                     AANGEILLTTYGRSSGFCVDPIEKKPLNHFLPGTPILSFGTAGCNLACKFCQNWDISK
                     SREWDTLADQAAPETIAAAARQLGCRSVAFTYNDPVIFMEYAMDVADACHELGIKAVA
                     VTAGYINAAARRDFFAHMDAANVDLKDFSESFYRDICAGELAPVLDTLRYLKHETAVW
                     FELTTLLIPGLNDSDTELDAMTRWVATELGPDVPMHFTAFHPDYKLLDRPPTPPATLS
                     RARTIALSNGVRYAYTGNVHDEAGGSTFCHACGERLIGRDWYTLTAWNLTADGHCRRC
                     GAACAGVFDGPPGRWGARRLPVRLGAYEE"
     gene            2558..>3309
                     /gene="amrB"
                     /locus_tag="PK829_11255"
     CDS             2558..>3309
                     /gene="amrB"
                     /locus_tag="PK829_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018877605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU41832.1"
                     /translation="MSNERVRPPAVAGRFYPADPQQLEADVRQYLNAAQAPDDCLAPP
                     LVKAIIAPHAGYVYSGPIAASAYIQLACSDIPVQRFVLVGPAHFAPLRGLATSSADAF
                     ETPLGRVPIDAAGREAALASPHVRIDNLAHRPEHCLEVQLPFLQVLFPDFTIIPLLVG
                     DAEAGAVADVLARLWGGPDTRIVISSDLSHYYSGAVARQLDAETAAAIVALDARALRD
                     DAACGREAIAGLLVEAQRHAMTAAALDLRHSG"
BASE COUNT          537 a         1162 c         1102 g          508 t
ORIGIN      
        1 tatgggcgcg gcggtcgcgc aacggccggc cacagaaggc cacgtccgcc gcccggccgg
       61 tttcgacgat gtgccagacg gtagcggaga gcataacgac ggggttggta atatccatcg
      121 ctagagatgg ttggcgcggg cgatgtggcc gatgggttcc ggcccgccca acaccgccac
      181 cacgtcgccc gcggccaggg cgcgctcggc cgccggatgg tggtcggagt ggccgtcccg
      241 ccgcaacagc acgacgctga cgtcgtaatc ccgctccacc gcgccgaccg acaacccatc
      301 cagccgcgag ccgcggccga cggtcaggct acacaggctg aagggctgcc cctcgatggt
      361 gatcggccgg gcaatgtcca tgcccgtggc cgcggcggcg aaggtcggcg cggccatgcc
      421 cgtggcgctc atggcccggt agccgaactg ccgcgccacc tcctcggcga aggcgtcgtc
      481 gaagatgcgc atgacgacct cgatgtcggg gttcagccgc cgggccatga aagccacttg
      541 caggttcagc acgtcgttct gggtgcagag gacgatggcc cgcgcccggg ccacggccgc
      601 gccgatcaac gtctcctgcc gctgcccgtc atcttgaatg accggcacgc gcagccgccg
      661 cgcctcatcg gccaggttgg ccttggggtt ctgctcgatg acgaccacgt cctgcccgcg
      721 ctcgaccaat tcctgcatga cgcggaagcc caggtggccc agaccgacga ggatgacgtg
      781 gttgctaaag gtcgaggcga cagccatttc ccactccttg cgccgggcgc ggcgattgaa
      841 gaacagccgc ccgaagtcgg ccagcccgaa ggccaggatg ccgatgccga cgatgggcat
      901 gacgaaatag aacagttgca gataccagac gtcggggaac tcgcccaacg gttgcaggaa
      961 gatcatctcc agcacgccgt agatggactc gattggcccg tccagccgct gcccggcgta
     1021 ggcggccagg gcgtagtaga gcagcccgcc gccgaccagc acgacgacga agagcagcag
     1081 cggccaggcg aactgccgca gcagcaccag ggtatcgcgc cacgccgccc gcgcgttgcg
     1141 ttgccaactg gccttcttga tcatgtcgga gatcgaacca tcgattgcac cgatgaagcg
     1201 ctcacgcaaa gaaagctcac gcaaagacgc aaagacgcaa agaagaatct tttctttgcg
     1261 tctttgcgcc tttgcgtgag cctcctactc ttcgtatgcc cccaggcgca ccggcagccg
     1321 ccgcgccccc cagcggccgg gcgggccgtc gaacacgccc gcgcaggccg ccccgcagcg
     1381 gcggcaatgg ccgtcggcgg tcaggttcca ggccgtcagc gtgtaccagt cgcggccgat
     1441 gagccgctcg ccgcaggcgt ggcagaaggt gctgccgccg gcttcgtcgt ggacgttgcc
     1501 ggtgtaggcg tagcggacgc cattggacag ggcaatcgtc cgcgctcggc tcagggtcgc
     1561 cggtggcgtg ggcggccggt cgagcagctt gtagtcgggg tggaaggcgg tgaagtgcat
     1621 gggcacgtct ggccccaact ccgtcgccac ccagcgcgtc atggcgtcca actcagtgtc
     1681 actgtcgttg aggccgggga tgagcagcgt cgtcagctca aaccagacgg ccgtctcgtg
     1741 cttcaggtag cgcagcgtgt ccagcaccgg ggccagttcg ccggcgcaga tgtcgcggta
     1801 gaacgactcg ctgaagtcct tcaggtcaac gttggccgcg tccatgtggg cgaagaagtc
     1861 gcgccgggcg gcggcgttga tgtagccggc ggtgacggcg accgccttaa tgcccaactc
     1921 atggcaggcg tcggccacgt ccatggcata ttccatgaaa atgaccggat cgttataagt
     1981 aaaggccacg ctacggcagc cgagctgccg ggccgccgcg gcgatggtct ccggcgcggc
     2041 ctggtcggcc agcgtgtccc attcgcgcga cttgctgatg tcccagttct ggcagaactt
     2101 gcaggccagg ttgcagccgg ccgtgccgaa cgacaggatc ggcgtgccgg gcaggaagtg
     2161 gttgagcggc ttcttctcga tgggatcgac gcagaagccg ctggagcggc cgtaggtggt
     2221 caggaggatc tcgccgttgg ccgcgccgcg cacgaagcac agcccgcgct ggccgtcgtg
     2281 gagccggcag gcccgcgggc acacgtcaca ctgtacccgg ccgtcggcca accggtgcca
     2341 gtgggtcgtt ggcgtgccgc tgatggttgg tgtcatacgt atttctcttg gcgcgccggg
     2401 gcgtggccca ggccatcgga gcgtccccgg caacacagat cggacatttg tattatacta
     2461 tgtctagacg acttggaagc ggccgattgt acggtggcca tccgcacgcc ccgcctgccc
     2521 tccggtcgct agaccccggt cgtcgcagga gaccactatg tccaacgaac gtgtgcgccc
     2581 gccggccgtg gccgggcgct tctaccccgc cgatccccag cagctagagg ccgacgtgcg
     2641 ccagtacctc aacgccgccc aggcccccga cgactgcttg gccccgccgc tggtcaaggc
     2701 catcatcgcc ccccatgccg gctacgtcta ctccggcccc atcgccgcct cggcctacat
     2761 ccaactggcg tgcagcgaca tccccgtgca gcgcttcgtc ctcgtcggcc cggcccactt
     2821 cgctcccctg cgcggcctgg ccaccagcag cgccgacgcc ttcgagacgc cgctcggccg
     2881 cgtgcccatc gacgccgccg gccgcgaggc ggcgctggcc agcccgcacg tccgcatcga
     2941 taacctggcc caccggccag agcactgcct ggaagtgcaa ctgcccttcc tgcaagtact
     3001 cttccccgat ttcaccatca tcccgctgct cgtcggcgat gccgaagccg gcgcggtcgc
     3061 cgacgtgctg gcgcggctat ggggcggccc ggacacgcgc atcgtcatca gttccgacct
     3121 gagccactac tacagcgggg ctgtggcccg gcagttggat gccgagacgg ccgcggccat
     3181 cgtcgccctc gacgcgcggg cgctgcgcga cgatgccgcc tgcggccggg aggccatcgc
     3241 cgggcttctg gtcgaggcgc aacggcacgc catgaccgcc gccgcgctcg acctgcgcca
     3301 ctccggcga
//
LOCUS       DAOSMQ010000364         3290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29181_length_3290_cov_5.252859, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000364 DAOSMQ010000000
VERSION     DAOSMQ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29181_length_3290_cov_5.252859"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PK829_11260"
     CDS             complement(<1..602)
                     /locus_tag="PK829_11260"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU41833.1"
                     /translation="MKPRWPVLVGILVAAVLLVGAGYVLANWRGAAAPAGIEIVPPAA
                     TATPAPPPTPLPTATPGPMRVYVSGAVARPAVYALPPGSIIDDAVRAAGGFTASADPV
                     AINLAAPVAEGMQIYVPTLAEDAPTPPPVSAPVATTAAEGGSRLGGVTTAGLININQA
                     TATELEMLPGIGPTMAANIVAHREANGPFATIEAIMDVPG"
     gene            complement(602..1585)
                     /locus_tag="PK829_11265"
     CDS             complement(602..1585)
                     /locus_tag="PK829_11265"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOU41834.1"
                     /translation="MRYVDNSDIHDPRLNLAFEEYLLRHVDSDEPLLLFYVNEPSVII
                     GRNQNTLEEIDPDYVNSHGIHVVRRLSGGGAVYHDLGNLNFSIVTNGKEHLNDFARFT
                     EPVVAVLRSLGVPAELRGRSDILVEGRKISGNAQYATTRRMFSHGTLLFDTDLGEMLR
                     AINPRRMQIESKAVQSVRSAVANIRELLPRDMTLADLRAVLLRGIFGGADIPTLDLTG
                     ADWLRIEDIAETRYRLWSWNYGRSPQFNVQKNARFPAGKVDARIDVEDGRVQAIRLFG
                     DFSGQRDVSELETHLVGVPYDRERLSAALADVNLDDYFGRMDVATLIDLLY"
     gene            complement(1598..3076)
                     /locus_tag="PK829_11270"
     CDS             complement(1598..3076)
                     /locus_tag="PK829_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU41835.1"
                     /translation="MTTLHRRLGSRRLGLALVALVLIALAWWGILAEGRGLTSRDLTG
                     AGGVPLTFIAPEGAAGAPGVVVAHGFAGSSRLMLGFGRALAHGGYGVMLLDFDGHGAA
                     RGPLDSSALQRNLQAAAEALAAQPEIDPTRLALLGHSMGSGAVMTAGITAADRYRATV
                     AVSPTGAEVSPDAPRNLLLMAGSLEPNFVGNARDLLSRAGGESADLAGGRGRALLVVP
                     NVEHITILFSRAAQTAALDWLDRAFGLPGGAAVAPDRRLVWYATHLGGWLLLLLAVAP
                     LIPAVAVVPAARRRPWAWLGLPVGALAAGLALLALGRMGDFTRLGGLLVGGVLALWFA
                     VLGLVWLGLLSGFRLPRLKGRDVLWGLLLFATLTVAFGLMADRVWIHWQLVPARLARW
                     PFLAAAAVPWLLAAGLAQAGASQPRRAGWWLWQTAVIVAGLALAIVVYPGLGFVSLLM
                     PIIPVILLILSVAGAAFDRPWSFALGGALFFGWLLVAVFPLA"
BASE COUNT          574 a         1134 c         1159 g          423 t
ORIGIN      
        1 atgccgggca cgtccatgat ggcctcgatg gtggcgaagg ggccgttggc ctcgcgatgg
       61 gcgacgatgt tggccgccat tgtcgggccg atgccgggca gcatctccaa ctccgtcgcc
      121 gtggcctggt taatattgat cagcccggcg gttgtcaccc cgcccaggcg gctgccgccc
      181 tccgcagcgg tcgttgccac cggggcgctg acgggcggcg gcgtgggcgc gtcctcggcc
      241 agcgtcggca cgtagatctg catcccctcg gccaccggcg cggccagatt gatggctacc
      301 gggtcggcgc tggcggtgaa gccacccgcg gcacgcacgg cgtcatcaat gatgctgccg
      361 ggaggcaggg cgtagacggc cggccgggcc accgcgccgc tgacatagac gcgcatgggg
      421 ccgggcgtgg ccgttggcag cggcgtgggc ggtggcgcgg gcgtggctgt ggccgccggc
      481 ggaacgatct cgatcccggc cggggctgcc gcgccgcgcc agttggccag cacgtaaccc
      541 gcgccgacca acaaaacagc ggccacaagg atgccgacca gaacaggcca acggggtttc
      601 atcagtacaa tagatcgatc agggtggcca cgtccatgcg accaaagtag tcatcgaggt
      661 tcacgtcggc caacgccgcg ctcagtcgtt cgcggtcata aggcacgccg acgaggtggg
      721 tctccagttc ggacacgtcg cgctggccgc tgaagtcgcc aaagaggcga atggcctgca
      781 cgcggccgtc ctccacgtcg atacgcgcat cgactttgcc ggccgggaag cgggcattct
      841 tctggacgtt gaattgcggc gagcggccgt agttccaact ccacagccgg tagcgcgtct
      901 cggcgatgtc ctcgatgcgc agccagtccg cgccggtcag gtcaagggta gggatgtcag
      961 cgccgccgaa gatgccgcgc agcagcaccg cccgcagatc ggccagggtc atgtcgcgtg
     1021 gcagcaactc gcggatgttg gccacggcgc tgcgcaccga ctggacggcc ttcgactcga
     1081 tttgcatccg ccgcggattg atggcccgca gcatctcgcc caggtcggtg tcgaagagca
     1141 gcgtgccgtg gctgaacatg cgcctggtcg tcgcgtactg ggcattgccg gagattttgc
     1201 gcccttccac cagaatatcg ctgcggccgc gcaactcggc cggcacgccc aacgaacgca
     1261 gcacggccac aaccggctca gtgaagcggg cgaagtcgtt gaggtgctcc ttgccgttgg
     1321 tgacaatgct gaagttgagg ttgcccaggt cgtggtaaac cgcgccgccg cccgacagcc
     1381 gccgcacgac gtggatgccg tgactattca cgtagtcggg gtcgatctcc tccagcgtgt
     1441 tctggttgcg accaatgatg accgacggct cgttgacgta gaacaatagc agcggctcgt
     1501 cgctgtccac gtggcgcagc aaatactctt caaaggccag gttcagccgg gggtcgtgga
     1561 tgtcgctgtt gtcgacgtag cgcatgaccg tatgctatca cgccagaggg aagacggcga
     1621 ccagaagcca gccaaagaag agtgccccgc cgagggcgaa gctccacggc cggtcaaagg
     1681 ccgcgccggc gacactcagg atcagcagga tgacggggat gatgggcatg agcaggctga
     1741 cgaagcccag gccgggatag acgacaatag ccagcgccag cccggcaacg atgacggccg
     1801 tctgccagag ccaccagccc gcccgtcgcg gctgcgacgc gccggcctgg gccagaccag
     1861 ccgccaacag ccacggcacg gccgccgcgg ccaggaacgg ccagcgcgct aaccgcgcgg
     1921 gcaccagctg ccagtgaatc cacacccggt cggccatcag gccaaaggca acagtcagcg
     1981 tggcaaagag gagcagcccc cagagcacgt cccggccctt cagccgcggc aggcgaaagc
     2041 cactgagtag ccctagccag accagcccca ggacggcaaa ccacagcgcc agcaccccgc
     2101 cgaccagcag cccacccagc cgcgtgaaat cgcccatgcg tcccagcgcc agcagcgcca
     2161 gaccggcggc cagcgccccc accggcagcc ccagccaggc ccacggccgc cggcgggccg
     2221 cgggaaccac cgccaccgcg ggaataagcg gcgcgacggc cagcagcaac agcagccagc
     2281 cgcccagatg ggtcgcgtac cacacgaggc ggcgatccgg agccacggcc gccccgccgg
     2341 gcaacccaaa ggcccggtcg agccaatcga gcgcggcggt ctgcgcggcg cggctgaaga
     2401 ggatggtgat gtgttcaacg ttggggacaa ccagtagcgc ccggccgcgc ccgccggcca
     2461 ggtcggcgct ctcgccgccg gcgcgggaca gcaggtcgcg ggcgttgcca acgaagttcg
     2521 gctccagcga gccggccatc agcaacaaat tgcgcggcgc gtcgggcgag acctccgcgc
     2581 cggtgggcga gacggccacg gtcgcccggt agcggtcggc agcggtgatg cccgcggtca
     2641 tgaccgcgcc gctgcccatg ctgtggccca gtagcgccag gcgcgtgggg tcgatctccg
     2701 gctgggccgc cagcgcctcg gccgcggcct gcaggttgcg ttgcagggcg ctgctgtcca
     2761 gcgggccgcg ggccgcgccg tggccgtcga agtcgagcag cataacacca tagccgccgt
     2821 gggccagagc gcggccgaag cccagcatga gccggctgga gccggcaaag ccgtgggcga
     2881 cgacaacgcc gggcgcgccg gccgcgccct ccggggcgat gaaggtcagg ggcacaccgc
     2941 ccgcgccggt caggtcgcgg ctggtcaggc cgcgcccctc ggccaggatg ccccaccagg
     3001 ccagggcgat gaggacgagg gcgacaaggg cgaggccaag gcggcgcgaa ccgaggcggc
     3061 gatgcagggt ggtcatgggc cgatcatagg ccaggccgcg ccgcctgtcc agcccgtagc
     3121 acagcctgtc aggctgtggc ccgtagcaca gcctgtcagg ctgcggccgg cgacgacagc
     3181 ctgtcaggct gcggcccgta gcacagcctg ttaggctgtg gccggcgacg acagtctaac
     3241 aggctgtgct acaggccaag atgtggtatt ctctgtctgt gcccacgcgg
//
LOCUS       DAOSMQ010000365         3284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29248_length_3284_cov_5.478476, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000365 DAOSMQ010000000
VERSION     DAOSMQ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29248_length_3284_cov_5.478476"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK829_11275"
     CDS             complement(<1..866)
                     /locus_tag="PK829_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU41836.1"
                     /translation="MTAISPPAARLSAAGRARQWLRDRNIPPVYGILIIIFIAAILLD
                     AFFGIDDELFILNQTILTNILVRSVALGIVAVGQTFVMVGASIDLSVAYLISVTAVGA
                     SYLMAGDPNRVWWTVLVLFVIGVLVGLVNGTLITRLRINPLIATLGVGLILRGLLNAT
                     FENFAGSVPASFQTFAYGMLGPLPISVLVLFLVVLVGWFVLRRMRYGAHLYAVGGSEE
                     VGRLSGLRTERVLTMAHVICSTTAVLTGLFVVSRLRAGAPWVGTDGLYDLESIAATVI
                     GGTALSGGKGGV"
     gene            complement(863..2458)
                     /locus_tag="PK829_11280"
     CDS             complement(863..2458)
                     /locus_tag="PK829_11280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU41837.1"
                     /translation="MAESFLTEAIEPAAAARPVARGVDPVLAVESITKRFPGVVALDD
                     VSVEFYPGEVHAVVGENGAGKSTLMKVLAGAYRPDGGTIRLDGAPVVFSHPSAAQAAG
                     ISIIYQEFNLLPERTVMENIFLGREPHRFGIVDYGRLEQDTRAVLQQLEAETLISPTA
                     LLGTLSVAQQQVVEIAKALSYDAKVLIMDEPTAALAQQEVTVLTALVRRLQARGIAII
                     FISHRLVEVFELAQRVTVLKDGQKVGTMPTKDLTMSKVVEMMVGRVLSDYFPERARPE
                     DIGAVALRLRGAGNATLRDIDLELRAGEIVGVAGLQGSGRTALAQAIFGVEPFQSGAM
                     EIQGQPELFRTPAQAIRNRFGYVTEDRKYEGLTLKQPINDNIMLTLRSLKAKLSRAFR
                     NGTRGDQELALELAQRVDVRAPGMNREVQFLSGGNQQKVVLAKWLATDARVLIFDEPT
                     RGIDVEAKASIHERIRELARAGAAVLMISSELPEVIGMSDRIVVMWDGAIAGELPAGA
                     GEADIMHLATGFAESSRNEEKSV"
     gene            complement(2461..>3284)
                     /locus_tag="PK829_11285"
     CDS             complement(2461..>3284)
                     /locus_tag="PK829_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU41838.1"
                     /translation="PEHLPLVARVAGLGFDVIEVAFEDPALIDVGALGQRAAAAGLGV
                     LVCGAYGPGRNLVSADPAERAATAAYVRALIDAAARLGSPVVSGPAYGAVGKTPAPDV
                     AARQAERDLAAAELRPLAAYAGARGVRLALEPLNRFETDLINVVEQGLALIEAVGSPH
                     LGLHLDTFHMHLEERDSAAAIRRAGDRLFHFHACENDRGTPGRGQVAWAAVAAALREV
                     GYDGAVVIESFTPEVKSIARAVCIWRELAGSQDELAGEGLAFLRTLLGDAARPER"
BASE COUNT          555 a         1167 c         1082 g          480 t
ORIGIN      
        1 cagacgccgc ccttgccgcc ggacagcgcc gtgccgccga tgaccgtggc ggcaatgctc
       61 tccaggtcgt agaggccgtc ggtgccgacc cagggcgcgc ccgcccgcag ccggctgacg
      121 acgaagaggc cagttaggac ggccgtcgtg ctgcaaatga cgtgggccat cgtcagcacg
      181 cgctccgtgc gcagaccaga cagccgcccc acttcctcgc tgccgcccac ggcatagagg
      241 tgcgccccgt agcgcatccg gcgcaacaca aaccagccca ccaacaccac caggaacaag
      301 accagcacgg agatgggtaa cgggccgagc ataccatagg caaaggtctg gaagctggcc
      361 ggcaccgaac cggcgaagtt ctcgaacgtc gcattcagca gcccgcgcag gatcagcccc
      421 acgcccagcg tggcgataag cgggttgatg cgcaaacggg tgatgagcgt gccgttgacc
      481 aggcccacaa ggacgccgat gacgaacaac accagtaccg tccaccagac gcggttgggg
      541 tcgccggcca tcagatacga cgcgcccacc gcggtgacgc tgatcaggta ggccaccgat
      601 agatcaatcg acgcgccgac catgacgaac gtctgcccca cggcgacaat gcccagagcc
      661 accgaccgca ccagaatgtt ggtcaggatg gtctgattga ggatgaacag ctcatcatcg
      721 atgccgaaga aggcatcgag cagaatggcg gcgatgaaga tgatgatcag gatgccatag
      781 acgggcggga tgttcctgtc ccgcaaccat tgccgcgccc ggccggcggc cgagaggcgc
      841 gctgccggtg gtgagatcgc tgtcatacgc tcttctcctc gttccgactg ctctcagcga
      901 atcccgtcgc caggtgcatg atgtcggcct cgcccgcgcc ggcgggaagc tcgccggcga
      961 tagcgccgtc ccacatgacc acgatccggt cgctcatgcc gatgacctcc ggtaactcgg
     1021 aagagatcat cagcacggcc gcgccggcgc gggccagctc gcgaatgcgc tcgtggatgc
     1081 tggccttggc ctccacgtca atgccgcgcg tcggctcgtc gaagatgagc acgcgcgcgt
     1141 cggtagccag ccacttggcc aacaccacct tctgctggtt gccgccgctc aggaattgca
     1201 cctcgcggtt catgcccggc gcgcgcacgt ccacgcgttg ggccagctcc agggccagtt
     1261 cctggtcgcc gcgcgtgccg ttgcggaagg cgcggctcag cttggccttc agcgagcgca
     1321 gcgtcagcat gatgttgtca ttgatgggct gcttcagcgt caggccctcg tacttgcggt
     1381 cttcggtcac atagccgaag cggttgcgaa tggcctgggc gggcgtgcgg aacagctccg
     1441 gctggccctg aatctccatc gcgccgctct gaaacggttc gacgccgaag atggcctggg
     1501 ccagcgccgt gcggcccgac ccctgcaggc cggccacgcc gacgatctcg ccggcgcgca
     1561 gctccagatc gatgtcgcgc agggtggcgt tacccgcgcc gcgcaaccgc agggccaccg
     1621 cgccgatgtc ctccggccgg gcgcgctccg ggaagtagtc ggacaggacg cggccgacca
     1681 tcatctccac gaccttgctc atggtcaggt ctttggtcgg catcgtgccc accttctggc
     1741 catccttcag cacggtcacg cgctgggcca actcaaagac ctctaccagc cggtgggaga
     1801 tgaaaatgat ggcgatgccg cgggcctgca agcggcgcac cagggcggtc agcacggtca
     1861 cttcttgctg ggccaacgcc gccgttggct cgtccatgat cagcactttg gcatcgtagc
     1921 taagggcttt ggcgatctca acgacctgtt gctgggccac cgacagcgtg cccagcaggg
     1981 ccgtggggct gatgagcgtc tccgcctcca gctgctgcag aacggcccgg gtgtcctgct
     2041 caaggcggcc atagtcgacg atgccgaagc ggtgtggttc gcgccccagg aagatgttct
     2101 ccatgaccgt gcgttcgggc agaaggttga actcctggta gatgatgctg atgccggccg
     2161 cctgcgccgc cgacgggtgg ctgaagacga ctggcgcgcc gtcgagccgg atggtgccgc
     2221 cgtcggggcg ataggccccg gccagcacct tcatcagcgt gctcttgccc gcgccgttct
     2281 cgccgacgac ggcgtggact tcgcccgggt agaactcgac cgagacgtcg tcaagagcca
     2341 ccacgccggg aaagcgtttg gtgatactct ccacggccag cacgggatcg acgccgcgcg
     2401 ccaccggccg ggccgcggcc gccggctcta tcgcttcggt cagaaagctc tcggccatag
     2461 ctacctctcc gggcgtgccg catcgcccaa cagcgttcgc aggaacgcca acccttcgcc
     2521 ggccagctca tcctggctgc cagccaactc gcgccagatg cacaccgcgc gggcgatgga
     2581 cttcacctcc ggggtgaagc tctcgatgac caccgcgccg tcatagccca cctcgcgcag
     2641 cgccgcggcc acggccgccc aggcgacctg cccgcgcccc ggcgtgccgc ggtcgttctc
     2701 gcaggcgtgg aagtggaaga ggcggtcgcc ggcgcgacgg atggccgcgg ccgagtcgcg
     2761 ctcctccaga tgcatatgga aggtgtccag gtgcagcccc aggtgcgggc tgcccacggc
     2821 ctcgatcagg gccagccctt gctcgacgac gttgatcaga tcggtctcga agcggttgag
     2881 cggctccagg gccaaccgga cgccgcgcgc gccggcataa gcggccagcg ggcgcaactc
     2941 ggccgcggcc agatcgcgct ccgcctgccg cgcggccacg tcgggcgcgg gcgtcttgcc
     3001 caccgcgcca taagccgggc cgctgacaac cggcgaaccg agccgggccg cggcgtcgat
     3061 gagcgcgcgc acgtaggccg ccgtggccgc gcgctcggcc gggtcggcgc tgaccaggtt
     3121 gcgccccggc ccatacgccc cgcagacgag cacgcccagc ccggcggcgg ccgcccgctg
     3181 gcccaacgcg cccacgtcga tgagggccgg gtcttcaaag gcgacctcga tcacgtcgaa
     3241 gccgaggccg gccacgcgcg ccaccagcgg caaatgctcc ggtg
//
LOCUS       DAOSMQ010000366         3283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29258_length_3283_cov_4.381970, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000366 DAOSMQ010000000
VERSION     DAOSMQ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29258_length_3283_cov_4.381970"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1951)
                     /locus_tag="PK829_11290"
     CDS             complement(260..1951)
                     /locus_tag="PK829_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOU41839.1"
                     /translation="MSTLSKPPAAGAAPSPEALSRGVDLLHDPLHNRGTGFSAAERQA
                     FGLRGLLPARVLSLEDQVARILENLRKKPTDLEKYVYLNDLVDRNETLFYRVLIDNLD
                     ELMPIVYTPTVGQGCQQYGHIFRRPRGLYISSEDAGHIAGLLANWPAEGVRVIVVTDG
                     ERILGLGDLGANGMGIPVGKLSLYTACAGIPPAMTLPIMLDVGTENAAFLADPLYIGV
                     PHPRLKGETYDAFIEEFMAAVTARWPKALVQFEDFANHHAFALLQRYRDRYRVFNDDI
                     QGTAAVTLAGLLAALRLIAVEGGPARRLSEQRLLFLGAGEAGVGIADLVVSAMMEEGL
                     SAEEARARCWFVDSHGLVVRSRADLAAHKLPYAHEHAPVAGLLAAVEELRPTAIIGVS
                     GQPRTFTREIIERMAKYNERPIIFALSNPTSKSECTAEEAYTYTGGRAIFASGSPFPS
                     VKLYGQTYNPGQGNNAYIFPGVGLGVVVSGASHVTDEMFEAAARTLADQVTADDMANG
                     RIYPTLSRIREVSLAIATAVAGIAHERGLATEPRPDDLSGYVREFMYNPTYDPLV"
     gene            2217..>3283
                     /locus_tag="PK829_11295"
     CDS             2217..>3283
                     /locus_tag="PK829_11295"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOU41840.1"
                     /translation="MDHSLAQTISLQCPQCGARFTAAVWLIVDAGARPELLARVADGA
                     VHTLVCPNGHDGEVDAPLLLYRPGETPALLFSPARRTTAEQDAEQAQGLIGRLRETLG
                     GAWRDEWLGELSPLPREALAALARGDEAAAAEVMQAAVAAALPSGVARALADIAGLLA
                     AEGVRVESAEDLERALAARPELRRRLEAALRAAGEGESDDEPAPPPPPPAAAPPPPAA
                     APPPPPPGPAPEEEELPPLLQALADFVNARTWLESYQFLLAHPELLSDELDALLGELL
                     AEAAASGQEHDVRLWTEHRALLQRSRATGAAAAFAEKMEMTAEELDKAVQEAEDLAAI
                     PPEARRAVEEIVAALAAEGVRP"
BASE COUNT          504 a         1054 c         1245 g          480 t
ORIGIN      
        1 gggaaacggc ggggcagcct cccgattcag cccattctac gccgaaccgg gcggccgtgg
       61 caactgcctg gatagggtta ttaccgagtt aacaggcgct cacgcaaagg tgctcacgca
      121 aaggcgcaaa gtaagcaaag gcgcaaagaa gaatgctcac gccaagacgc caagcttaag
      181 aacccttctt aactttgcgt tctttgcggc tttgcgtgag cattctttgc gtgagcgttt
      241 cctccgactt gggaactgcc tagaccagtg gatcgtaagt cgggttgtac atgaattcgc
      301 gcacgtagcc ggacagatcg tccggccgcg gctcggtggc caggccgcgc tcgtgggcaa
      361 tgccggccac ggccgtggcg atggccagcg acacctcgcg gatgcgcgac agggtcgggt
      421 agatgcggcc gttggccatg tcgtcggcgg tcacctggtc ggccagcgtg cgcgccgcgg
      481 cctcgaacat ctcgtcggtc acgtggcttg cgccgctgac gacgacgccc agcccgacgc
      541 cggggaagat gtaggcgttg ttgccctggc cgggattata ggtctggcca tagagcttga
      601 ccgacgggaa ggggctgccg ctggcgaaga tggcccggcc gccggtgtag gtgtaggcct
      661 cttcggcggt gcactcggac ttggaggtcg ggttcgacag ggcgaagatg atcggccgct
      721 cgttgtactt ggccatgcgc tcgatgatct cgcgggtgaa ggtgcgcggc tggccgctga
      781 cgccgatgat ggccgtcggc cgcagctcct ccaccgcggc caggaggccg gccaccggcg
      841 cgtgctcgtg ggcgtagggc agcttgtggg cggccaggtc ggcgcgcgac cgcaccacca
      901 gcccgtggga atcgacgaac cagcagcgcg cgcgggcctc ctcggccgac agcccttcct
      961 ccatcatggc cgaaacgacc aggtcggcaa tgcccacgcc ggcctcgccc gcgcccagga
     1021 acagcagccg ctgctcgctg agccgccgcg ccggcccgcc ctcgacggcg atgagccgca
     1081 gcgccgccag cagcccggcc agcgtcaccg cggccgtgcc ctggatgtcg tcgttgaaga
     1141 cgcggtagcg gtcgcggtag cgctgcagga gggcaaaggc gtggtggttg gcgaagtcct
     1201 cgaactgcac cagcgccttc ggccagcgcg ccgtcaccgc ggccatgaac tcctcaatga
     1261 aggcgtcgta ggtctcgccc ttcagccgcg ggtggggcac gccgatgtag agcgggtcgg
     1321 ccagaaaggc ggcgttctcc gtgcccacgt cgagcatgat cggcagggtc atcgccggcg
     1381 ggatgccggc gcaggcggtg tagagcgaca gcttgcccac ggggatgccc atgccgttgg
     1441 cccccaggtc gcccagaccg aggatgcgct cgccgtcggt gacgacgatg acgcgcacac
     1501 cttcggccgg ccagttggcc agcaggccgg cgatgtggcc ggcgtcctcc gacgagatgt
     1561 agaggccgcg cggccggcgg aagatgtggc catactgctg gcagccctgg ccgacggtcg
     1621 gcgtgtagac gataggcatc agctcgtcga gattgtcgat gaggacacgg tagaagagcg
     1681 tctcgttgcg gtcgaccagg tcgttgagat agacgtactt ctccagatcg gtcggcttct
     1741 tgcgcagatt ctccaggatg cgggcgacct ggtcctccag cgacaggacg cgcgccggca
     1801 gcaggccgcg caggccgaac gcctggcgct cggcggcgct gaagccggtg ccgcggttgt
     1861 ggagcgggtc gtggagcaag tcaacgccgc gagacaacgc ctcgggcgac ggggccgcgc
     1921 cggcggccgg gggcttggac agggtactca tgatcactct ccgatagacg cccggaaggg
     1981 gcgcgcgctc gcgccgccgg ccgccgccgg ccggaacggc gacaatgagg tgggtggaaa
     2041 ggtgggttac gcgggtagtg tacacggttt ttgcgggagt ggatagggga gagtgggcag
     2101 tgggcagtgg gcagtgggca gtatccagtg ggcagtggac agtatccagt cggtggtcgg
     2161 cggtcgtccg ttgccatcca accggctaca atccacccaa cgacaggagg tcgccggtgg
     2221 atcattccct tgcccagacc atttcattgc aatgccccca gtgcggggcg cgtttcacgg
     2281 ccgcggtgtg gctgatcgtc gatgccgggg cgcggccgga gttgctggcc cgcgtcgctg
     2341 atggggcggt gcacacgctc gtctgcccca acggccacga cggcgaagtg gacgcgccgc
     2401 tgctgctcta ccggccgggc gagacgccgg cgctgctctt ctcgccggcg cggcggacga
     2461 cggccgagca ggatgccgag caggcccagg ggctgatcgg ccggctgcgc gagacgctgg
     2521 gcggcgcttg gcgcgacgaa tggctggggg agctgtcgcc gctgccgcgc gaggcgctgg
     2581 cggcgctggc ccgcggcgac gaggccgccg cggctgaggt gatgcaggcg gccgtggccg
     2641 cggccctgcc gtcgggcgtg gcccgggcgc tggccgacat cgccgggctg ctggcggccg
     2701 agggcgtgcg cgtcgagtcg gccgaagacc tggagcgggc gctggcggcg cggccggaac
     2761 tgcgccggcg gctggaggcg gcgctgcggg cggcgggcga gggcgagagc gacgacgaac
     2821 cggccccgcc gccgccgcca ccagcggccg cgccgccgcc accagccgcc gcgccgccgc
     2881 cgccgccacc cggccccgcg ccggaagagg aagagttgcc gccgctgctg caagcgctgg
     2941 ccgactttgt caacgcgcgg acatggctgg agtcttacca gtttttgctg gcccacccgg
     3001 aattgttgag tgatgaactg gatgcccttc tcggcgagct tctggcggaa gcggcggcaa
     3061 gcggacaaga gcacgacgtc cggctgtgga ccgagcaccg cgccctactg caacgcagcc
     3121 gtgcgacggg cgcggcggcg gcctttgccg agaagatgga gatgacggcc gaggaactgg
     3181 acaaggctgt acaggaagct gaagacctgg cggccatccc gccggaggcg cgccgggcgg
     3241 tggaggagat cgtggccgcg ctggcggccg agggtgtgcg gcc
//
LOCUS       DAOSMQ010000367         3283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29259_length_3283_cov_4.210967, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000367 DAOSMQ010000000
VERSION     DAOSMQ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29259_length_3283_cov_4.210967"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK829_11300"
     CDS             <1..532
                     /locus_tag="PK829_11300"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU41841.1"
                     /translation="SALADMARYYPQSAGSLTQMQGVGAAKQARYGDAFLAVIRAYCA
                     ERDIAERPKVGAMTRAAGPAVPGKTRRDEVLALYQSGRGVAEIGGIFGIKLATVANHL
                     WEAVQDGVSIDPTPLLADTGLSAADQARALQAFRELGAEKLRPVYDALGETISFDALH
                     LLRLYAVCTALQRATG"
     gene            complement(529..1188)
                     /locus_tag="PK829_11305"
     CDS             complement(529..1188)
                     /locus_tag="PK829_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU41842.1"
                     /translation="MEPTESSLDTLRRTELPTLGTSIPRRGNRLTRIFFTLLLRLMGW
                     RVVGQLPDLPRLVFVGAPHTSNGDFFMTALTMAALGIDVHFVMKHTPFVGPVGWFLRW
                     FGGIPLDRDHTRDFVSQMVDVFDSREQFLLAIMPEGTRSVVKGWRSGFYHIAHNAGVP
                     LVMVKFDYPNRRMRVGPVFWPSGDYETELPLIQAEFADIAGRHPELMLALKETTVAKA
                     I"
     gene            1425..3227
                     /locus_tag="PK829_11310"
     CDS             1425..3227
                     /locus_tag="PK829_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOU41843.1"
                     /translation="MTTGSFVPDWAKGIVWYQIFPERFRNGDPTNDPTLASLEGAWPH
                     DFTSPWQVHPWTADWYRLQPYEQQNGRDIFFNLQRRRYGGDLQGILDSLDYLTDLGVE
                     ALYLNPVFDAPSAHKYDGTTYHHIDPHFGPDPDGDRRLIAAETPHQPATWLWTAADRL
                     ALRLIEEVHWRGLRIIFDGVWNHMSRRSWVFRDVAARQQQSPYRDWLNVASWDDAAQG
                     TSFECHGWAGVPELPQLRQDANGIVSGGEATGGFGPREYIFAATRRWLDPDGDGDPRD
                     GIDGWRLDVAYCVRHPFWQAWRRHVRSINPQAYLVAEVVLPPEDEQPYLRGDEFDATM
                     NYNFAFAAMEFLVGGAGATTATEFDARLRELRGAYPAEVAHVMQNLIDSHDSSRLASA
                     IVNRDLHPMRDWPEYHRLSRVEMSPDYATRKPTAAERRRQMLVALFQFTYIGTPMLYY
                     GDEAGMWGANDPCCRKPMVWPEMPYEPESTLPDGRPRPHANAVAFDHDLFAHYQTLIA
                     LRKASPALRRGEFRTVLADDGRRLFAFVRETDDDTAIVVLNADDLMQIATLELPAGRW
                     TDALSGESYWTEAGRLSLTVPPLWGAVLRPAASP"
BASE COUNT          572 a         1108 c         1092 g          511 t
ORIGIN      
        1 cagtgccctg gccgatatgg cccgctacta cccgcagtcg gccggcagcc tgacgcagat
       61 gcagggcgtt ggcgcggcca agcaggcgcg ctacggcgac gccttcctgg ccgtcatccg
      121 cgcctactgc gctgagcggg acatcgccga gcggcccaag gtgggcgcga tgacgcgggc
      181 agccgggccg gccgtccccg gcaagacgcg gcgcgatgag gtgctggctc tctaccagag
      241 cgggcgcggt gtggctgaga tcgggggaat tttcgggatc aaactggcaa cggtggccaa
      301 ccatctctgg gaggcggtgc aggacggcgt gagcatcgac ccaacgccgc tgctggccga
      361 tacagggcta tcggccgccg accaggcccg cgccctacag gcgttccgcg agctgggcgc
      421 ggagaagctg cggccggtct atgacgccct gggcgagacg atcagcttcg atgccctgca
      481 cttgctacgg ctctatgccg tctgcacggc cctgcaacgg gcgacgggct agatcgcctt
      541 ggcgacagtc gtctccttca gggcaagcat cagttccggg tggcggccgg cgatgtcggc
      601 gaactcggcc tggatgagag gcaactcggt ctcgtagtcg ccactgggcc agaagacagg
      661 gccaacgcgc atgcggcggt tggggtagtc gaacttgacc atcaccagcg gcacaccggc
      721 gttgtgggca atgtggtaga agccgctgcg ccaacccttg acaacgctgc gcgtcccttc
      781 gggcatgatc gccagcaaga attgctcgcg gctgtcgaac acatcgacca tctgcgacac
      841 gaagtcgcgc gtgtggtcgc gatcaagggg aatgccaccg aaccagcgca ggaaccagcc
      901 caccggcccc acaaagggcg tatgcttcat gacaaaatgg acatcgatgc ccagagcagc
      961 catcgtgagg gcggtcatga aaaagtcacc attggacgtg tgcggcgcgc cgacaaacac
     1021 caggcgcggc aggtcgggca attgtccaac gacgcgccag cccatcaggc gcagcagcag
     1081 cgtgaagaag atgcgcgtca gacggttgcc gcggcggggg atgctggttc ccagggttgg
     1141 tagttcggtc cggcgaaggg tgtcgagcga ggactcagtt ggctccatag ctgtggtggc
     1201 tcctgcaagt gccggtgacg cccggtctat cggcagcggt ttgattacaa ccggtaaaac
     1261 cccgccgggc gacaaaagac acgcccccct caccaatgtc agacctggcc agtctcagaa
     1321 ggccgccgcg gtcgggcctc taagaggtct ggggcgttct tgtggcccag ggcgctcttt
     1381 tccggcatga tcgcgccaaa cccatccgcc aaggagcagc aacaatgacc accggcagct
     1441 tcgtgcccga ctgggccaag ggcatcgtct ggtatcagat cttcccggag cgcttccgca
     1501 acggcgaccc gaccaatgac ccaacgctgg ccagcctgga gggcgcctgg ccccacgact
     1561 tcacctcgcc ctggcaggtg catccctgga cggccgactg gtatcgcctc cagccctatg
     1621 agcaacagaa cgggcgcgac atcttcttta acttgcagcg gcggcgctac ggtggcgact
     1681 tgcaggggat tctcgacagc ctcgactacc tgaccgacct gggggtggag gccctctacc
     1741 tcaaccccgt cttcgacgcg ccctcggctc acaaatacga cggcacgact tatcaccata
     1801 tcgaccccca cttcggcccc gaccctgacg gcgaccggcg gttgattgcc gcggagacgc
     1861 cccaccagcc ggccacctgg ctgtggacgg cggccgaccg gttggcgctg cgcctcatcg
     1921 aggaggttca ctggcgcggc ctgcgcatca tctttgacgg cgtttggaac cacatgagcc
     1981 gccgcagctg ggtcttccgc gacgtggccg cgcggcaaca gcagtccccc taccgcgact
     2041 ggctcaacgt ggcctcttgg gacgacgccg cgcagggcac aagcttcgag tgccacggct
     2101 gggcgggcgt gcccgagttg ccgcaactgc gccaggacgc caatggcatc gttagcggcg
     2161 gtgaggcgac gggcggcttt ggcccgcgcg agtacatctt cgccgccacg cgccgctggt
     2221 tggaccccga cggcgacggc gacccgcgcg acggcatcga cggctggcgg ctcgacgtgg
     2281 cctactgcgt ccgccacccc ttctggcagg cgtggcggcg gcacgtgcgc agcatcaacc
     2341 cacaggccta cctggtggcc gaggtcgtcc tgccgccgga agacgagcag ccctacctgc
     2401 gcggggacga attcgatgcg acgatgaact acaatttcgc cttcgcggca atggagttcc
     2461 ttgtcggcgg cgcgggggcc acgacggcaa ctgagttcga cgcccgcctg cgcgagttgc
     2521 gcggggccta cccggccgag gtcgcccacg tgatgcaaaa tctgatagac agccacgaca
     2581 gcagccggct ggcctcggcc atcgtcaacc gcgacctgca ccccatgcgc gactggccgg
     2641 aataccaccg cctgtcgcgc gtggagatgt cgcccgacta tgccacgcgc aagcccacgg
     2701 ccgccgagcg ccgccggcag atgcttgtgg ccctgttcca gttcacctac atcggcacgc
     2761 cgatgctcta ctacggcgac gaggcgggca tgtggggggc caacgatccc tgctgccgca
     2821 agccgatggt ctggccggag atgccgtatg agccggagag cacactgccc gacggccgcc
     2881 cgcgtcccca cgccaacgca gtggcctttg accacgatct gttcgcccac tatcagacgc
     2941 taatcgccct gcgcaaagcg tcgccggcgc tgcggcgcgg tgaatttcgc accgtgctgg
     3001 ccgacgatgg ccggcggctg tttgctttcg tccgcgagac agacgatgac acggccatcg
     3061 tcgtcctgaa cgccgatgac ctgatgcaga tcgctacgtt ggaactgccc gccgggcgtt
     3121 ggacggacgc gctgagcggg gagagctatt ggaccgaggc ggggcggctg agcctgactg
     3181 tgccaccgct ctggggggcg gttctgcggc cggccgccag cccgtagcac agcctgtagc
     3241 acagcctgtt aggctgtggc cggcgacgac agcctgacag gct
//
LOCUS       DAOSMQ010000368         3276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29337_length_3276_cov_3.522819, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000368 DAOSMQ010000000
VERSION     DAOSMQ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29337_length_3276_cov_3.522819"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="PK829_11315"
     CDS             complement(<1..1281)
                     /locus_tag="PK829_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41844.1"
                     /translation="MRFSRLTLAIVAAISCVSFGWALAQGGTEPASDGGQIPALIDAS
                     VGRGFTYQGYLTESSAPHNGTCDFRFKLWDASTGGNQIGATQTVTQVALTGGRFTALV
                     DDADQFGPTAFDGNARWLEVAARCSIASDFITLTPRQALRGVPYALGLRPGAVISGEV
                     PSSGVLVLNNSADNGLALKSDSGGIAVETAFVGVRVANAVNDGFQAQSAFASGFSVGT
                     AGIDGLYINTAGKDGVAIDTSGDNGVIVNQAGGAGINVNQAGESGFRIGLTAGDGLLV
                     GNAAKAGVKVMNASTGLAVYGDDTSIGLLIQPENPDWAGVFDGDVQITGSCTGCRLAT
                     IGVNVGNRPLQPGDIVAIQGVSPGVQGQADLLWQVTPFEPGLTPIGVVAGRMELVTYD
                     HLSPDGQPAQYLAPRPGAVMPGGTVDIITYGPVQV"
     gene            1654..2100
                     /locus_tag="PK829_11320"
     CDS             1654..2100
                     /locus_tag="PK829_11320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOU41845.1"
                     /translation="MSMKHLSGLELMQAMVAGNLPMPPIAHIIPMKGVVAEHGRIVFE
                     AQADERHLNPLGGVHGGFAATVLDSVTGCAVHTVLEPGAGYGTVDLNVKMLKAVPRGV
                     PLIAEGRVLFVSRSLGVSEGTLKDAEGNLYAHATATCLILRPSSAS"
     gene            2105..2974
                     /locus_tag="PK829_11325"
     CDS             2105..2974
                     /locus_tag="PK829_11325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU41846.1"
                     /translation="MTKILENKVAVITGSTRGLGLAIAQTFAAEGAAVVIAGRTPAPV
                     AAAVAALQDGGARAAGLACDVGDREQVKALGTLAVERFGGIDIWVNNAGLSAPYGPTA
                     EIAPDAFRRVVDTNIVGVYNGSMVALGHMLPQGRGKMINLLGRSRKNGVKYQNAYSAS
                     KVWVASFTLALAAEYEDSGVGIFAFNPGLVDTDMLRRVDVVRGYEQKVKPLETVMRLW
                     ANQPELPAQRALWLASSATDGKTGLSVNVLSRTSAMMGLAREGVRRLTGRAAPDTSLE
                     IRSVAPDDEFAGR"
     gene            3038..>3276
                     /locus_tag="PK829_11330"
     CDS             3038..>3276
                     /locus_tag="PK829_11330"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU41847.1"
                     /translation="MSRTRLPTDQILSLLAAAPGHIAAAAAGLSPEQLRTAPTPGEWS
                     ANDILAHLRACADVWGDCIATILAEEHPTIRAMNPR"
BASE COUNT          598 a         1060 c         1057 g          561 t
ORIGIN      
        1 cacttgaacg gggccatagg tgatgatatc gactgtgcca cctggcatca ccgcgcccgg
       61 tcttggagct aaatactggg ccggttgtcc gtcggggctg aggtgatcat acgtgaccag
      121 ttccatgcga ccggccacca ctccgattgg cgtgagtcca ggctcgaagg gcgtcacctg
      181 ccacagcaag tctgcctgac cctgtacgcc tggactcacc ccctgaatag cgacgatgtc
      241 gcccggctgg agcggccggt tgcccacatt gacgccaatc gtcgccaagc gacaaccggt
      301 gcatgagccg gtgatctgga catccccatc aaacacaccc gcccaatcgg ggttttccgg
      361 ttgaattagg agaccaatac ttgtgtcatc gccatacacc gccagaccgg ttgaggcatt
      421 catcaccttg accccggcct tggcggcatt cccgaccaat aggccgtcgc ctgctgttaa
      481 accgatgcga aagccgcttt ccccagcctg gttgacgttg ataccggcgc cgccggcctg
      541 gttgacgatg accccattat cgccgctcgt gtcaatcgcg acgccatctt ttccggcagt
      601 gttgatgtac aggccgtcaa tacctgcggt gcccacagag aaaccactgg cgaaggccga
      661 ttgcgcctgg aacccatcgt taaccgcatt cgctacccgg acgccaacaa aggccgtctc
      721 tacagctatg ccgccgctat ctgacttcag ggcgagtcca ttgtcggcgg agttgttgag
      781 caccagcacg ccactagagg gaacttcgcc actgatgact gcccccggcc tcaatcccag
      841 ggcgtaaggc acgccgcgca aagcctggcg tggggtgagg gtaatgaaat cgctggcgat
      901 tgaacagcga gccgccacct ccagccagcg cgcattgccg tcgaaagctg tggggccaaa
      961 ctggtcggcg tcatcgacca gggcagtgaa gcggccgccc gtcaacgcga cctgggtaac
     1021 tgtctgggtg gcgccaatct ggttgccgcc ggtcgaggcg tcccacaact tgaagcggaa
     1081 gtcacaggtg ccgttgtggg gcgcgctgct ctccgtcagg tatccttgat aggtgaagcc
     1141 gcggccgacg gaggcgtcga tcagggccgg aatctgcccg ccgtcgcttg ccggctccgt
     1201 acctccttga gccaaggccc agccgaaact gacacaactg atggcggcaa cgatggcaag
     1261 ggtgagtcgg gaaaatcgca tgaaattacc tccggggcgg tccttatgag actgctagat
     1321 gattttctgc gcgaccaacc gataggaagg ggcattgcgc cccagaaagt attataatga
     1381 ctctggtgac aaatgatagc ccgttgatca tcgcgcccgg ccacttcaga cgacacaatg
     1441 gccaaccggg ctgcattcat ttgtggggga agcatggaac aacccgtcgg gccggacaac
     1501 agcgcgacgg cgcgccggct gcccgtttca cgcggcgctt ggtcgaactg attgagagtg
     1561 gctacgggct gagcgtagcg aatgaggtgg gcgatgatgg cagcagcagc gagccgctac
     1621 cgcggttggc ccatgaactg gcggggtagg gccatgtcga tgaagcacct gtccggcctc
     1681 gaactcatgc aagccatggt ggcgggcaac ctccccatgc cgcccatcgc ccacatcatc
     1741 cccatgaaag gcgtggtcgc cgaacacggc cgtatcgtct tcgaggccca ggccgacgaa
     1801 cgccacttga acccgctcgg cggcgtgcat ggtggttttg ccgccacggt gctggactca
     1861 gtgacgggtt gcgccgtcca taccgtgctg gagccgggcg ccggctatgg cactgtcgat
     1921 ctgaacgtga agatgctgaa ggccgtcccg cgcggcgtgc ccctgatcgc cgagggccgg
     1981 gtgctcttcg tctcgcggtc gttgggtgtg tccgagggga cgctcaagga cgccgagggc
     2041 aacctctacg cccatgccac ggcgacgtgc ctgatcctgc gcccctcgtc agcgtcgtga
     2101 aatgatgacg aagatactgg aaaacaaggt agcggtcatt accggcagca cgcgcggtct
     2161 ggggctggcc atcgcccaga cctttgcggc tgagggcgcg gccgtggtca ttgccggccg
     2221 cacgccggct ccggtcgccg ccgccgtcgc tgccctacag gacggcggcg cgcgggcggc
     2281 cggcctggct tgcgacgtgg gcgaccgcga gcaggtgaag gcgctgggca cgttggccgt
     2341 cgagcgcttc ggcggcatcg acatctgggt caacaatgct ggcctgtccg cgccctatgg
     2401 gccgacggcc gagatcgcgc cggacgcctt caggcgcgtg gtcgatacga atatcgtcgg
     2461 cgtctacaac ggttcaatgg tcgccctggg ccacatgttg ccccaggggc gcggcaagat
     2521 gatcaacctc ctcggccgca gccgcaagaa tggcgtcaag taccagaatg cctacagcgc
     2581 cagcaaggtc tgggtggcca gcttcaccct ggcgctggcg gcggagtacg aagatagtgg
     2641 cgtgggcatc ttcgccttca atcccggcct ggtcgatacc gacatgttgc ggcgcgtcga
     2701 tgtcgtgcgg ggctacgagc agaaagtcaa gccgctggag acggtcatgc gcctgtgggc
     2761 caaccagccc gaattgccgg cccagcgggc gctctggctg gcttccagcg ccactgacgg
     2821 caagacggga ctgtcggtga acgtgctcag ccgaacgagc gcgatgatgg gcctggcgcg
     2881 cgagggcgtc cgccgcctga cgggccgggc cgcgcccgac acctcgctgg agattcgctc
     2941 cgtcgcgccc gacgatgagt ttgccgggcg atgaggcgca tagcggccgt ggcccacgcc
     3001 gcttagcggc cgtggcccac gccactagag aaggccgatg agtagaacac gacttcccac
     3061 tgaccaaatt ctcagcctgc tggccgccgc ccccggccat atcgccgcgg cggcggccgg
     3121 gctgtcgccg gaacaactgc ggacggcgcc cactcccggc gaatggtcgg ccaacgacat
     3181 cctggcccac ctgcgcgcct gtgccgacgt gtggggcgac tgcatcgcca ccattctggc
     3241 cgaggagcat ccgaccatcc gggccatgaa cccgcg
//
LOCUS       DAOSMQ010000369         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29418_length_3268_cov_5.033925, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000369 DAOSMQ010000000
VERSION     DAOSMQ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29418_length_3268_cov_5.033925"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1040
                     /locus_tag="PK829_11335"
     CDS             195..1040
                     /locus_tag="PK829_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU41848.1"
                     /translation="MVKIITDTTACLSPEIARRYDIPVIPQIVHFGDETFYEGVDMDA
                     EAFMNRLKASAALPQTAAPPPGLFVEQFRRWAPLGEPIVCLHPSAEISGTIRSATVAA
                     AEFPDADIRIVDTRLVASALGTIVQLAAEAAAQGADAGTIVARANDLSRRGRIYFLVS
                     TLEYLARGGRIGGAAALVGSILSVKPILQLQDGRIEQYERERTQKRALARLKEIVREQ
                     IPGDGRGYLSILYAGNPDEGRALADDLGQAINQPDVPVVSMPPAIITHGGPGLLGAAF
                     FVANA"
     gene            complement(1043..2455)
                     /locus_tag="PK829_11340"
     CDS             complement(1043..2455)
                     /locus_tag="PK829_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOU41849.1"
                     /translation="MTDHMTPDDFRRHGKALIDWIADYYEHIETYPVLSRATPGSVRA
                     ALPEGPPQEGEPFAAVMADVERAILPGITHWQSPNFFAYFPANASFPSILGELLSAGL
                     GVQGMIWQTSPACTELETLVLDWLVGMLGLPEAFLSTHSGGGVIQDTASSAVLAALLA
                     ARERATGYSNVESGNDPRLRAYCSTQTHSSVSKAAGVMGVGRANLRAIAVDAAYAMRP
                     EALRAAIEADLAAGLRPFFVCATVGTTSSTAIDPIRAIGTVAREYGLWLHVDAAMAGT
                     AALCPEFRPLQDGLELADSYAFNPHKWMFTNFDCDCFWVADRAALIRTLTILPDYLKN
                     AATASGAVIDYRDWHVQLGRRFRALKLWFVIRHYGVTGLQARVREHVRLAQQFAAWVA
                     ADARFELAAPVPLNLVCFRLRDGGDDANRALLERLNASGKLYLTHTVLDGKYTLRLSI
                     GGTLTEERHVQAAWARIREL"
     gene            2656..>3268
                     /locus_tag="PK829_11345"
     CDS             2656..>3268
                     /locus_tag="PK829_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU41850.1"
                     /translation="MADKSLNKGAAGLDETTWGHRWGYADTRFVVNPDRSVKVTGHRY
                     AVSGYDMPGFLPFVEEMLGVQIDLSDVKPERMDKPVPAPALNAAFSTALAAVLAPAQI
                     STDARERLIHSHGQTTADEVYQVLYTHLDRVVDMVIYPETQAQVEALIVLAAAHDVCL
                     VPYGGGTSVSCALKLPASETRTIVSVDMRRMDAIEWIDRENCRA"
BASE COUNT          559 a         1102 c         1073 g          534 t
ORIGIN      
        1 tttctgctgt ccaggatcaa ctcccatgcc gcggccatag gctgttcggc cgtctgcgtt
       61 tgcctccccg cggaagcgcc cggtgtgtcg ccaacaactg gcgcgctccc caaatccgcg
      121 tacgattccg acaacctggt tcagcggcgc gccccaacca gagaacactg gtttcacacg
      181 gagggttatc actcatggtc aaaatcatca ctgataccac cgcctgtctt tcgccggaaa
      241 tcgcccgccg ctacgatatc ccagtcatcc ctcagatcgt ccatttcggc gacgaaacct
      301 tctatgaagg cgttgacatg gacgccgaag cgtttatgaa ccggctaaag gcgtcggcgg
      361 cgttacccca gacggccgcg ccaccgccgg ggctttttgt cgaacagttc aggcgatggg
      421 cgccgctggg tgagccgatt gtgtgcctcc atccgtcggc cgaaatcagc ggcaccatcc
      481 gctcggctac cgtcgccgcc gccgagttcc ccgacgccga catccgcatc gtggatacgc
      541 gcctggtggc cagcgcgttg gggacaatcg tgcagctggc ggccgaggcg gcggcgcagg
      601 gcgcggacgc tggcaccatc gtcgcccgtg ccaacgatct gagccggcgc ggccggattt
      661 atttcctcgt cagtacgctg gaatacctgg cccgtggcgg gcggatcggc ggcgcggcgg
      721 cgctggtggg cagcatcctg tccgtcaagc cgatcttgca attgcaggac ggccgcatcg
      781 agcagtatga gcgcgaacga acccaaaaac gagccctggc gcgactgaaa gagatcgtcc
      841 gggaacagat tcccggcgac ggccgcggct atctgtccat cctgtatgcc ggcaacccgg
      901 acgaaggtcg tgccctggcc gacgatctcg gccaggccat caaccagccc gatgtgccgg
      961 tagtgtccat gcccccggcc atcatcaccc acggcgggcc ggggctgctg ggcgcggcct
     1021 tcttcgttgc caacgcctga aatcaaagct ctcgtatcct ggcccacgcc gcttgcacgt
     1081 ggcgctcttc ggtcagcgtg ccgccaatgg acaggcgcag ggtatacttg ccgtccagca
     1141 ccgtgtgggt caggtacagc ttgccggagg cattcagacg ttccagcagc gcccgattgg
     1201 cgtcgtcgcc cccatcgcgc aggcggaagc agaccagatt caagggcacg ggcgcggcca
     1261 gctcgaagcg cgcgtcggcc gccacccagg ccgcgaactg ctgcgccagg cgcacgtgtt
     1321 cgcgcacccg cgcctgtagc ccggtcacgc cgtagtggcg gatgacgaac cacagcttca
     1381 gcgcgcggaa gcggcggccg agttgcacgt gccagtcgcg atagtcgatg accgcccccg
     1441 acgccgtggc cgcgttcttc aggtaatcgg gcaggatggt cagggtgcga atgagcgccg
     1501 cccggtcagc cacccagaag cagtcgcagt cgaagttggt gaacatccac ttgtgggggt
     1561 tgaaggcgta gctgtcggcc agttccaggc cgtcttgcag cgggcggaat tccgggcaga
     1621 gggctgccgt gccggccatg gccgcgtcga cgtggagcca cagaccgtac tcgcgggcga
     1681 cagtgccgat ggcccggatg gggtcgatgg ccgtggagga ggtcgtgccc accgtggcgc
     1741 aaacgaagaa cggccgcagc ccggcggcca ggtcggcctc aatggccgcg cgcagggcct
     1801 ccgggcgcat ggcgtaggcc gcgtccacgg cgatggcgcg caggttggcc cggcccacgc
     1861 ccatcacccc ggcggcctta ctgaccgacg agtgcgtctg cgtggagcag taggcccgca
     1921 ggcgcggatc gttgccactc tcgacgttgc tatagccggt ggctcgttct ctggccgcca
     1981 ggagggcagc caataccgcg ctgctggcgg tgtcttgaat cacaccgccg ccggagtgcg
     2041 tggacaaaaa cgcctccggc aggccgagca tgcccaccag ccagtcgagg acaagcgttt
     2101 ccaattcggt gcaggcgggg ctggtctgcc agatcatccc ctgcacgccc agcccggccg
     2161 aaagcaactc gcccaggatg gacggaaagg aggcgttggc cgggaagtag gcgaagaagt
     2221 tgggcgactg ccagtgggtg atgcccggca ggatggcccg ctccacgtcg gccatcacag
     2281 cggcgaacgg ctcgccctct tgcggcggcc cctccggcag cgccgcgcgg acgctgcccg
     2341 gcgtggcccg cgagaggaca gggtaggtct caatgtgctc atagtaatcg gcgatccagt
     2401 cgatcagcgc cttgccgtgc cggcggaagt cgtcgggggt catgtggtcg gtcatggtct
     2461 gtctccgggg atgagatggt cgtagcgtaa cgcaaaggag gagcagtggc cagtggtcat
     2521 ggccagtggc cagtggtcag tgggccgtaa gcgctctgcc taccgagcac tagccctgcc
     2581 cactgccatc ttttgccccc cggcggcgat tgccgcataa tggccactga tccatcgcat
     2641 cattggagcg aactcatggc cgacaaatca ctgaacaagg gcgccgccgg cctcgacgag
     2701 accacttggg gtcaccgctg gggctatgcc gacacccgct tcgtcgttaa ccccgaccgc
     2761 agcgtcaaag tcaccggcca tcgctacgcc gtctccggct acgacatgcc cggtttcctg
     2821 ccgttcgtcg aggagatgct cggcgtacag atcgacctga gcgacgtcaa gccggagcgc
     2881 atggacaagc ctgtgcccgc gccggcgctc aacgccgcct tcagcaccgc gctggcggcc
     2941 gtcctcgccc cggcgcagat cagcaccgac gcccgcgagc gcctcatcca cagccacggc
     3001 cagacgacgg ccgatgaggt ctaccaggtg ctctacactc atctcgaccg cgtcgttgat
     3061 atggtcatct atccggagac gcaggcccag gtggaggcgc tcatcgtcct ggccgcggcc
     3121 cacgacgtct gcctggtgcc ttatggcggc ggcacgagcg tcagttgtgc cctgaagctg
     3181 ccggccagcg agacgcgcac catcgtctct gttgatatgc gccgcatgga cgcgatcgag
     3241 tggatcgacc gggagaactg ccgcgcct
//
LOCUS       DAOSMQ010000370         3255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29568_length_3255_cov_5.323750, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000370 DAOSMQ010000000
VERSION     DAOSMQ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29568_length_3255_cov_5.323750"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PK829_11350"
     CDS             complement(<1..1070)
                     /locus_tag="PK829_11350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU41851.1"
                     /translation="MKFQDFSLDARIEAGIHDAGYVTPTPIQEQAIPLVLEGRDVLGL
                     AQTGTGKTAAFMLPILQRLTRGPLRRTRVLIIAPTRELAEQIHQTAVVLGRATKARSA
                     AIYGGVGKGPQVAALQRGTEIIVACPGRLLDHIHAGDVDLSGIEVLVLDEADRMLDMG
                     FLPDIRRILKHVPPKRQSMLFSATMPADIRSLADSILTDPVTVQVDMRAPAKTVSHAL
                     YPVPETLKNKLLHALLEQTAMSRVLVFTRTKHRAKRLALDLGKQGYRVAAIQGNLSQN
                     RRQEAMDGFRGGKYDILVATDIAARGIDVSEISHVINYDMPDTVDAYTHRIGRTGRAE
                     ATGEAVTFAVQDDEPLVRDIERV"
     gene            1318..>3255
                     /locus_tag="PK829_11355"
     CDS             1318..>3255
                     /locus_tag="PK829_11355"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HOU41852.1"
                     /translation="MFIRRFLPLFPILLLSLLLPVVGGQAAPVAEWSPAGSLNAADGG
                     ALHHFGTAVAIDGDTAIVGAPGWPSAPDARPGAAYVFTHNGTAWVQRTRLTANDGQHG
                     DGFGNSAVLVGNVLVVGAVSARRGNPGDPNIAYGVGAVYSYTGSGATWTQETKLMPAD
                     LVDNNLFGASLAFDGETLVVGAPYGDVGHEAVYFYTRRAGGWDGPVKFDSPDPTVNND
                     FGQGVAVAGDLALVGAPGIDCCDANAAFTKGVVYVFARHAGVWSPAGTLSPDDGRLGD
                     NFGCDIAFDGTTAVVAACQAYGLAPTLHGYAYVFSRAGDTWTQTARLEPEGDATGSFG
                     IRAVALAGDQLVLGWPSAPPEGATDRLYPYTRTGATWTAGEPIESPDGPVDTDFGWHL
                     ALSETHLLAGAPYLAKLNVEEQGRVYAYWQPGATTGHILYAPIIASPGLESPDGLIAY
                     NIHEENGRFAIYLIRPDGTGKHPLMANREVNEDSPAWSPDGARLAFTESSPGQNPLLV
                     TTNVAGGDRRVVPLADVRDPGSPSWSPDGTRLAFVAWTKNYTRDDVYVVNLDGSGLVN
                     RTLTMSGRPQEPRWSPDGTKLLFVYDDRSSGPLQLAVVGATSGSPVVLTDNDMHHSSP
                     AWSPDGTRILFATMASGSTQVL"
BASE COUNT          509 a         1108 c         1081 g          557 t
ORIGIN      
        1 acgcgctcga tgtcacgcac cagcggctcg tcgtcttgca cggcgaaggt gaccgcctcg
       61 cctgtggcct cggcgcggcc ggtgcggccg atgcggtggg tgtaggcatc gaccgtatcg
      121 ggcatgtcgt agttgatgac gtgcgagatc tcgctgacgt cgatgccgcg cgcggcgatg
      181 tccgtggcca cgaggatgtc gtacttgccg ccgcggaagc cgtccatggc ctcctgccgc
      241 cggttctggg acaggttgcc ctgaatggcg gccacgcgat agccctgctt gcccaggtca
      301 agcgccagcc gcttggcgcg gtgcttggtg cgcgtgaaga ccagcacgcg cgacatggcc
      361 gtctgctcca gcagggcgtg cagcagcttg ttcttcagcg tctccggcac cgggtagagg
      421 gcgtgggaga cggtcttggc cggggctctc atgtccacct gcaccgtcac cgggtcggtc
      481 aggatgctgt cggccaggga gcgaatgtca gccggcatgg tggccgagaa gagcatcgac
      541 tgccgtttgg gcggcacgtg cttcaggatg cggcggatgt cgggcaggaa gcccatgtcg
      601 agcatgcggt cggcctcgtc gaggacgagc acctcgatgc cggacaggtc cacgtcgccg
      661 gcgtggatgt ggtcgagcag gcggccgggg caagcgacga tgatctccgt gccgcgttgc
      721 agcgcggcca cctgcggccc tttgcccacg ccgccataga tggcggcgct gcgcgccttc
      781 gtggcccggc ccagcacgac ggccgtttgg tgaatctgtt cggccagttc ccgcgtcggg
      841 gcaatgatga gcacccgcgt ccgccgcaat ggcccgcgcg tcaggcgctg caagatgggc
      901 agcatgaagg ccgctgtctt gcccgtgccc gtctgggcca ggccaagcac gtcgcgccct
      961 tcgaggacga gggggatggc ctgttcctga atgggggtcg gcgtcacgta gccggcgtca
     1021 tgaatgccgg cctcgatccg tgcgtcgaga gaaaagtctt gaaacttcac tgagaatact
     1081 ccttgggaca tcaccgagcc aagtcgcact cccagccgat gtttcttttt cgtccaataa
     1141 tgattgtggc aaccagccct ttgggccgcg atgccggggc gcagtatagc aggccggccg
     1201 gcgcgctgtc aagctggcgc gtcactgctc ataatctgga ggttccaact gtggtatcat
     1261 atatcttgag tgggggccgc tcgcgattct cctaacacct taaccacagg agccaccatg
     1321 tttatcaggc gttttctacc cttgttcccg atcctcttgt tgtccctgtt gctgcccgtc
     1381 gtgggcggcc aggccgcgcc ggttgccgag tggtcgccgg ccggcagtct caatgccgcc
     1441 gatggcggcg cgttgcacca tttcggcacg gctgtcgcca ttgacggcga cacggccatc
     1501 gttggcgcgc ccggttggcc aagcgccccg gacgcaaggc ccggcgcggc ttacgtcttc
     1561 acccacaacg gcacggcctg ggtccagcgg acgcggctga cggccaacga cgggcaacac
     1621 ggcgacggct tcggcaactc ggccgtactc gtgggcaacg tcctggtcgt cggagccgtt
     1681 agcgcgcgtc gcggcaatcc gggcgatccc aacattgcct acggggtagg cgcggtctac
     1741 agctataccg gcagcggcgc gacgtggacc caggagacga agctgatgcc ggccgatctg
     1801 gtcgacaaca acctattcgg cgccagcctc gccttcgacg gcgagacgct cgttgtcggc
     1861 gcgccctatg gcgacgtggg ccatgaggcc gtctacttct acacccgccg cgccggcggc
     1921 tgggatgggc cggtcaagtt cgatagccct gacccaaccg ttaataacga tttcggccaa
     1981 ggggtggctg tcgccggcga cctggccctg gtcggcgcgc cgggcattga ttgttgtgac
     2041 gcgaacgcgg ccttcacaaa gggcgtcgtc tacgttttcg cccgccacgc gggcgtctgg
     2101 tcgccggcgg gcacgctgtc gcccgacgac ggccgcctcg gcgacaactt cggctgtgac
     2161 atcgccttcg acggcacaac ggccgtcgtg gccgcctgcc aggcctatgg gctggcgcca
     2221 actctccacg gctacgccta cgttttcagc cgcgctggcg acacctggac gcagaccgcc
     2281 cgcctggagc cagaaggcga cgccaccggc tcgtttggca tccgcgccgt cgccctggcc
     2341 ggcgaccagt tggtgctggg ctggcccagc gcgccgccgg agggcgccac cgaccggctc
     2401 tacccctaca cgcgcaccgg ggcgacgtgg acggccggag aacccatcga atcgcccgac
     2461 ggcccggtcg atacggactt cggctggcac ctggcgctga gcgagaccca tttgctggcc
     2521 ggcgcgccct atctggccaa gctgaatgtt gaagagcagg gccgcgtcta tgcctactgg
     2581 caacccggcg cgaccaccgg ccacatcctc tacgcgccga tcatcgcctc gcccggtctg
     2641 gaaagccccg acggcctgat cgcctataac attcacgaag agaacgggcg cttcgccatc
     2701 tacctgatcc ggcccgatgg cacaggcaag caccccctga tggccaacag ggaagtaaat
     2761 gaagattcgc ccgcctggtc gcccgacggc gcgcgcctgg ccttcacgga gagcagcccg
     2821 ggccagaacc cactgctcgt cacaaccaac gtcgccggcg gcgaccggcg cgtcgtgccg
     2881 ctggccgatg tgcgcgaccc ggggtcgccc tcctggtcac ctgatggcac gcggctggcc
     2941 ttcgtcgcct ggacgaaaaa ctacacccgc gatgacgtct acgtggtcaa tctggacggc
     3001 agcgggctgg tcaaccgcac gctgaccatg tccggccggc cgcaagagcc gcgctggtcg
     3061 cccgatggca caaaactgct ttttgtctac gacgaccgca gcagcgggcc gctgcaattg
     3121 gccgtcgtcg gcgccacgag tggtagcccc gttgtcctca ctgacaacga catgcaccat
     3181 agctcgccgg cctggtcgcc cgacgggacg cgcatcctct tcgcgacgat ggccagcggc
     3241 tccacgcagg tgctg
//
LOCUS       DAOSMQ010000371         3249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29644_length_3249_cov_5.228554, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000371 DAOSMQ010000000
VERSION     DAOSMQ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29644_length_3249_cov_5.228554"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK829_11360"
     CDS             complement(<1..566)
                     /locus_tag="PK829_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU41853.1"
                     /translation="MPPTHAANHHNRAYLALALGLVGMGFSGIFVKLAGTPGAVSAFY
                     RVAVAVALLALPFSRGIARDGQPSRREALIAVLAGLFFAGDLFFWNTGILISGATTPT
                     LMGNTAPIWVGLGAMVFFGERPGRLFWVGLAIAIGGAVVILGVDALNDVGLGTFFGVL
                     SGMFYGGYFLVVQRSRQKLNTLTSFWLSA"
     gene            779..1510
                     /locus_tag="PK829_11365"
     CDS             779..1510
                     /locus_tag="PK829_11365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU41854.1"
                     /translation="MSELLRDKVAVVTGASRGIGEATALTLAQAGANVVVTARSADEL
                     AGLAERLERMDVGALAVPADVTQEADVERLRAAALARFGAIDILVNNAGVGKYGPLSS
                     LTAADYDWMMNTNMRSSFLCTAAFLPDMLARRQGWLVFIASVAGLKGLPNESVYCATK
                     FAQMGFAQALDYETREQGVKVSVIAPGGVGTHFAFGTGRTPGDPALDAMLDAQDVAEA
                     VLFAVTQPAKARVFLIGMRPMAEAL"
     gene            1629..2087
                     /locus_tag="PK829_11370"
     CDS             1629..2087
                     /locus_tag="PK829_11370"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU41855.1"
                     /translation="MEQLFTDMSERLETLVRAIETAVDGVPAEDLDWKPASDMNSLAV
                     LLTHTAGALRYWVGEVAGGQPAGRVRAQEFETHSVDADTLLGRLREALDHSKSVLANL
                     SLAQLNETRVGGMDNIPHSAGWALLHALEHTGIHTGHIQMTRQLWDARDK"
     gene            2109..2900
                     /locus_tag="PK829_11375"
     CDS             2109..2900
                     /locus_tag="PK829_11375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41856.1"
                     /translation="MTADNDAARQAWETNAAVWDERMGMAGNDFVNVLIWPRTQRLLD
                     VRPGERVLDIACGNGLYALRLADLGAQVTAFDFSAPLIERARAHAAGYGDRIALRVLD
                     ATDGDALLALGEGQFDAAMCNMALFDMADIGPLAGAVARLLRPGGRFVFSVMHPCFNG
                     LHVTHVTESADDGRQWTTRYALKLSRYLTPFTAEGIALRGQPEPQPYFHRPLSVLLAP
                     FLGAGLALDALEEAAFPPNHEPGQTTSWSGNYTEFPPVLIARLRR"
     gene            2952..>3249
                     /locus_tag="PK829_11380"
     CDS             2952..>3249
                     /locus_tag="PK829_11380"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOU41857.1"
                     /translation="MTDEMTKTALMERIGESWEALQRVLAPLDEAQLSRPDPASGWAI
                     KDHLIHLATGERGIAWLLSGRSRYEAMSITADEWHSLEMDQVNDLVYQRHRERTA"
BASE COUNT          561 a         1064 c         1153 g          471 t
ORIGIN      
        1 gccgacagcc agaaggaggt cagcgtgttg agcttctgcc ggctgcgctg gacgacgagg
       61 aaatagccgc cgtagaacat gcccgacagg acgccgaaga acgtgcccag gccgacatcg
      121 ttgagcgcgt ccacacccag aatgaccact gcgccgccga tggctatggc cagcccgacc
      181 cagaacagcc gccccggccg ctcgccgaag aagaccattg cccccaggcc gacccagatg
      241 ggcgcggtat tgcccatcag cgttggcgtc gtcgccccgc tgatgaggat gcccgtgttc
      301 cagaagaaaa ggtcgccggc aaagaacagc ccggccagga cggcgatgag cgcttcgcgc
      361 cgcgacggct gcccgtcgcg ggcaatgccc cggctgaagg gcagcgccag cagcgccacg
      421 gccacagcca cgcggtagaa ggccgacacc gcgcccggcg tcccggccaa cttgacgaag
      481 atgccggaga agcccatgcc gacaagaccc agggccagag ccaggtaggc gcggttgtgg
      541 tggttggcag catgggtggg gggcatggcg tgtccgtgtt gaccggttgg agcgcgtgcg
      601 ggcgcggggc acttataccg caggcgcggg caatcggcaa tgctcgcccg tggcgcgatt
      661 ctcccgaatc gcgctcttcg cgcgccgcga ttcggagaat cgcgccacga tttgggagaa
      721 tcgcgccacg gctgggcggc gctgctatac tatgctggcg agcgaaggag acaaagcaat
      781 gagtgagcta ttgcgggaca aggtggcggt ggtgaccggg gccagccgcg gcatcggcga
      841 ggccacggcg ctgacgttgg ctcaggccgg ggccaacgtt gtcgtgacag cccgctcggc
      901 cgatgagttg gccggcctgg ccgaacgcct ggagcgcatg gatgtcggcg cgctggctgt
      961 gcccgccgat gtgacccagg aggccgatgt ggagcgattg cgcgcggcgg cgctggcccg
     1021 cttcggcgca atcgacatcc tggtcaacaa cgccggtgtc ggcaagtatg gcccgctcag
     1081 cagcctgacc gcggccgatt acgactggat gatgaacacc aacatgcgca gcagcttcct
     1141 gtgcacggcc gctttcctgc ccgacatgct ggctcgccgg cagggctggc tggtcttcat
     1201 cgcctccgtc gccgggctga aggggctgcc caacgagagc gtctattgcg ccacgaagtt
     1261 cgcccagatg ggctttgccc aggcgctgga ctacgagacg cgcgagcagg gcgtcaaggt
     1321 cagtgtcatc gcccccggtg gcgtgggcac gcacttcgcc ttcggtaccg gccgcacgcc
     1381 cggcgacccc gccctcgacg ccatgctcga cgcgcaggac gtggccgagg cggtgctctt
     1441 cgccgtcacg cagccggcca aagcgcgggt gttcctgatt ggcatgcggc cgatggcgga
     1501 ggcgttgtaa cggatgaagg atgagggatg acggaaagtg gacgacggac gacggaccac
     1561 gggtggaaga tgtctgtggt cggccgtcgg ccgtcggcgg tcgaagacaa catgaaggag
     1621 tgagcggcat ggaacagcta tttacggaca tgagcgagcg gttggaaacg ttggtgcggg
     1681 cgattgagac ggccgtcgat ggcgtgccgg ccgaggactt ggattggaag cccgcctcgg
     1741 atatgaactc gttggccgtc ctgctgacgc atacggccgg ggcgctgcgc tactgggttg
     1801 gcgaagtggc cggcgggcag ccggcggggc gcgtgcgggc gcaggagttt gagacgcaca
     1861 gcgtggacgc ggacaccctg ctggggcgtt tgcgcgaagc gctcgaccac agcaaaagcg
     1921 tgctggccaa tctcagcctg gcccagttga acgagacgcg cgtgggcggc atggacaaca
     1981 taccccacag cgccggttgg gcgctgctcc acgcgctgga gcacaccggc atccacaccg
     2041 gccacatcca gatgacgcga cagctctggg acgcgcgcga caagtagacg aagggccgcg
     2101 atacgaccat gactgccgac aacgacgccg cccgccaggc ctgggaaacc aatgccgccg
     2161 tctgggacga gcgcatgggc atggccggca acgattttgt caacgtgctc atctggccgc
     2221 ggacgcagcg gctgctggac gtgcggccgg gcgagcgcgt cctcgacatc gcctgcggca
     2281 acggcctcta cgcgctgcgg ctggccgacc tgggcgcgca agtgaccgcg ttcgacttct
     2341 ccgcgccgct aatcgagcgc gcccgcgccc acgccgccgg ctacggcgac cgtatcgccc
     2401 tgcgcgtcct cgacgccacc gacggcgacg ccctgctggc tttgggcgag ggccagttcg
     2461 acgcggccat gtgcaacatg gccctgttcg acatggccga catcgggccg ctggccgggg
     2521 cggtggcgcg gctgctgcgg ccgggcgggc ggttcgtctt ctcggtcatg cacccgtgct
     2581 tcaacgggct gcacgtgacc cacgtaaccg agagcgccga cgacggccgg cagtggacga
     2641 cgcgctacgc cctgaagctg tcccgctacc tgacaccgtt caccgccgag ggcatcgccc
     2701 tgcgcggcca gccggagccg cagccctact tccaccgtcc gctgagcgtt ctgctggccc
     2761 cgtttctggg cgcgggtctg gctctcgacg cattggagga ggccgccttt ccgccaaacc
     2821 acgagccggg ccaaaccacc tcgtggagcg gcaactacac tgagttcccg ccggtgctca
     2881 tcgcccggct gcggcgctga ggccgcgtcg ccaacaccca ggtgacgacc gacattctcc
     2941 gtggaggcaa gatgaccgac gaaatgacga agacggcgct catggagcgc attggcgaga
     3001 gttgggaagc gttgcagcgc gtgttggctc cgctggacga ggcgcagttg agccggcccg
     3061 acccggccag cggttgggcg atcaaagacc acctgatcca cctggcgacg ggggagcggg
     3121 gcattgcctg gctgctgagc ggtcgttccc gctacgaggc catgagcatc accgccgacg
     3181 agtggcacag cctggagatg gatcaggtca acgacctggt ctaccagcgc caccgcgagc
     3241 gcacggccg
//
LOCUS       DAOSMQ010000372         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29840_length_3232_cov_4.034624, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000372 DAOSMQ010000000
VERSION     DAOSMQ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29840_length_3232_cov_4.034624"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PK829_11385"
     CDS             <1..516
                     /locus_tag="PK829_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOU41858.1"
                     /translation="HLGPLSLKQEGLQFAIRSAGRFITVVSSFLLLTFTTRPDGLMLA
                     LSQRGFPNSIAYIMLATMQIVPRFQTKANTILDAQRSRGLETEGNLLVRLRALLPLVQ
                     PLLLGSIIDIEERAIALEVRAFGRPGPKTSLLVLHDTPAQRVLRWLLLLAMLAVIGWR
                     LYLAIAPRLAA"
     gene            513..1349
                     /locus_tag="PK829_11390"
     CDS             513..1349
                     /locus_tag="PK829_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41859.1"
                     /translation="MIRLGDVTYFYPRATTPALAGVTLEIPTGQFCAVIGANGAGKST
                     LCYTLAGFVPHFYRGKLSGGVEVAGVDVPASSLAQLAGHVGLVFANPFNQITGARFSV
                     REEIAFGLENMGVPLEEMATRVEDILGFAGLRDLAERSPFALSGGQQQRLAIASVLVM
                     RPPVIVLDEPTSQLDPIGSREVFAVLRDLAATDAATVVLAEQKLEWVAVFADRVIVLH
                     EGRIVADGLPGDVLASADLAQYGLQPTRYTQAARAAQAAGFRADRDDLPVTLEEAVEF
                     FR"
     gene            1346..2218
                     /locus_tag="PK829_11395"
     CDS             1346..2218
                     /locus_tag="PK829_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41860.1"
                     /translation="MTAGGQRTTDDIGAATGAALAPMPIVVANVSFTYPSGTQALRGV
                     SLRVAPGEAVAIVGENGAGKTTLVKHLNGLLKPSEGSVVVGDWDTREHTVAKMAARVG
                     YVFQNPDDQIVERTVGDEVAFGPRRLGQPEAERQANVAAALATVGLTDAAATHPYDLT
                     TPQRKLVTLASVLAMRTPIVIFDEPTMGQDAGGVALIGGIVESLKAEGRTVLTITHDI
                     DFCAAHFGRAIVMAAGQALADGPAAEVLTHADVLATTNVEPPQLVRLAQALRLKRAPL
                     TVEGFVAEIVGRGQ"
     gene            2304..>3232
                     /locus_tag="PK829_11400"
     CDS             2304..>3232
                     /locus_tag="PK829_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU41861.1"
                     /translation="MVIILGDILADYAIRVEQLQLEPKDLQRVSYLALGPGGACNVAI
                     MAAHLGLKVAALGEVGDDLFGEIVLEGLADEGVDVSGVIISPGARTPVASVLVDERGE
                     PAYLGYPGSLQVMSLSEAWRPTIGTAEALYADGWADHEGVPAIVLAALRVAREAGVPT
                     FFDPGPGNPKIDNAWHSEAAALATVLLATEDEARRLSGEADPVNSARVLLERGTQLAV
                     IKRGMAGAFLLTADDLHIAPGLPVVARDATGAGDSLAAAIIYGTLHHFDLQTLGDIGN
                     ATGAAKVLKLGTGHNMPTVAEVNEMLRRFGAEVL"
BASE COUNT          487 a         1111 c         1125 g          509 t
ORIGIN      
        1 cacctggggc cgctgtcgct gaagcaggag ggcttgcagt ttgccatccg cagcgccggc
       61 cgcttcatca ccgtcgtcag cagcttcctg ctgctaacct tcaccacccg gcccgatggc
      121 ctgatgttgg cccttagcca gcgcggcttc cccaacagca tcgcttacat catgctggcg
      181 acgatgcaga tcgtgccccg cttccagacc aaagccaaca ccatcctgga cgcccagcgc
      241 tcgcgcggcc tggagacgga gggcaaccta ctcgtgcggc tgcgggcgct gctgccgctc
      301 gtccagccgc tgctgctggg cagcatcatc gacatcgaag agcgggccat cgccctggaa
      361 gtgcgcgcct tcggccggcc ggggccgaag accagcctgc tcgtgctcca cgacacgccc
      421 gcccagcgcg tcctgcgctg gctgctgctg ctggccatgc tggccgtcat cggctggcgg
      481 ctctatctgg ccatcgcgcc gaggttggcg gcatgatccg gctgggcgat gtgacctact
      541 tctacccgcg ggcgacgacg cccgcgctgg ccggcgtgac gctggagatt cccacggggc
      601 aattctgcgc cgtgatcggc gccaacggcg cgggcaaatc cacgctgtgc tacaccctgg
      661 ccggcttcgt gccccacttc taccgtggca agctgtctgg cggggtggag gtcgccggcg
      721 tcgatgtgcc ggcctcgtcg ctggcgcaac tggccggcca cgtcggcctg gtcttcgcca
      781 atccgttcaa ccagatcact ggcgcgcgct tcagcgtgcg cgaggagatc gccttcgggc
      841 tggagaacat gggcgtgccc ctggaagaga tggcgacgcg cgtcgaggac attctcggct
      901 ttgccgggct gcgcgacctg gccgagcgct cgcccttcgc cttgtccggc gggcaacagc
      961 agcggctggc catcgcctcg gtgctggtca tgcgcccgcc ggtcatcgtt ctcgacgagc
     1021 cgacgtcgca actcgacccc attggctcca gggaggtctt tgccgtcctg cgcgacctgg
     1081 cggcgaccga cgcggccacg gtggtgctgg ccgagcagaa gctagagtgg gtggccgtct
     1141 ttgccgaccg cgtgattgtg ctgcacgaag ggcgtattgt ggccgacggg ctgccgggcg
     1201 atgtgttggc ctcggccgac ctggcccaat acggcttgca accgacgcgc tatacccagg
     1261 ccgcccgcgc cgcccaggcc gccggcttcc gcgccgaccg agacgacctg cccgtgacgc
     1321 tggaagaagc cgtggagttc tttcgatgac cgccggcgga caacggacga cggacgacat
     1381 aggggcggcg actggcgcgg ccctcgcgcc catgcccatt gtggtggcga acgtgagttt
     1441 cacctacccc agcggcacgc aggccctgcg cggcgtcagc ctgcgcgtgg cccctggcga
     1501 ggccgtggcc atcgtcggcg aaaacggcgc gggcaagacg acgctggtga aacaccttaa
     1561 cggcctgctg aagcccagcg aggggagcgt ggttgtcggc gactgggaca cgcgcgagca
     1621 tactgtggct aagatggccg cccgcgtcgg ctacgtcttt cagaaccccg acgaccagat
     1681 cgtcgagcgc accgtgggcg acgaggtcgc ctttgggcca cgccgtctgg ggcaaccgga
     1741 ggccgagcgg caggccaacg tggccgcggc gctggcgacc gtcgggctga ctgacgcggc
     1801 ggctacccac ccctatgacc tgaccacgcc acagcgcaag ctagtcacgc tggcgtcggt
     1861 gctggccatg cgcacgccca tcgtcatctt cgacgagcca acgatgggcc aggatgccgg
     1921 cggtgtggcc ctcatcggcg gcatcgtcga gagcctgaag gccgaggggc gcacggtgct
     1981 gaccattacc cacgacatcg acttctgcgc ggcccacttc ggccgggcca tcgtcatggc
     2041 cgccggccag gcgctggccg atggcccggc ggccgaggtg ctgacccacg ccgacgtgct
     2101 ggccacgacc aacgtcgagc cgccgcaact cgtccgcctg gcccaggcgc tgcggctgaa
     2161 gcgcgccccg ctgacagtgg aggggtttgt ggcggagatt gtgggaaggg ggcagtaggc
     2221 agtaggtcag taaacagtag gtcagtaggt cagtgggcgg cggtcggcgg tcggcggtca
     2281 gcgatagcac ggaggctctc ctcatggtca tcatcctcgg cgacattctg gcagactatg
     2341 ccattcgggt ggagcaactc caactggagc ctaaggactt gcagcgtgtc tcctacctgg
     2401 ctctagggcc agggggggcg tgcaatgtgg ccatcatggc cgcgcatctg ggattgaagg
     2461 tggccgcgct gggcgaggtg ggcgacgatc tctttggcga gatcgtcctc gaagggctgg
     2521 ccgatgaggg cgtcgatgtg agcggcgtca tcatcagccc cggcgcgcgg acacccgtgg
     2581 ccagtgtgct cgtcgacgag cgcggcgagc cggcctacct gggctacccc ggcagcttgc
     2641 aggtgatgag cctgtcggag gcctggcggc caacgattgg cacggccgaa gccctctatg
     2701 ccgatggctg ggccgaccac gagggcgtgc ccgccatcgt cctggcggcg ctgcgcgtgg
     2761 cccgcgaggc gggcgtgccg accttcttcg accccggccc cggcaacccc aagatcgaca
     2821 acgcctggca cagcgaagcc gccgcgctgg cgactgttct gctggcaacc gaggacgagg
     2881 cccgccgcct gtcgggcgag gccgaccccg tcaactcggc ccgcgtcttg ctggagcggg
     2941 gaacgcaact ggcggtcatc aagcggggca tggccggggc gtttctgctg acggccgacg
     3001 acctgcacat tgcccccggc ctgcccgtcg tggcccgcga cgccaccggc gcgggcgaca
     3061 gcctggccgc ggcgatcatc tacggcaccc tgcatcactt cgacctccag accctcggcg
     3121 acatcggcaa cgccaccggc gcggccaagg tgctgaagct gggcacgggc cacaacatgc
     3181 ccaccgtggc cgaagtcaac gagatgttgc ggcgatttgg tgcggaggtc ct
//
LOCUS       DAOSMQ010000373         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29920_length_3224_cov_3.955506, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000373 DAOSMQ010000000
VERSION     DAOSMQ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29920_length_3224_cov_3.955506"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PK829_11405"
     CDS             <1..1210
                     /locus_tag="PK829_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOU41862.1"
                     /translation="AELQLDNNQYFLNVPVAADLAVDPLYFGSRPGSANAVVLEATVF
                     NLSAVAAANVPVAFYNTNGQQLVVVATATISSLPAGGSAIATGTWANPPAQYAAMVVV
                     DPAGQVPDSNRENNAASLTPGFEVVGKAATVTAVVGTSVTFPVEVRSVGGYSNSVALE
                     ALDLPPGGTATVTPASVAPPGTATVEITIPADAPPAVYPVRVVGVSGERSATAIVNVN
                     VVLTLMCYPLTVTVQGQGAPPTLSPERSPDCPGAGDYVAGQVIDLTAQPAAGWRVSAW
                     TGTLDDASQSPANAAIMPESNHTVGVVYAPLCYSLTLQKEGEGALPVAAPNKSSECAA
                     NGQYKPGELITLTASPAATWRVAGWRGTGNDGASASTNQLTMPAADHVAGVVYERNVR
                     LLWLPVLLGP"
     gene            1426..2376
                     /locus_tag="PK829_11410"
     CDS             1426..2376
                     /locus_tag="PK829_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOU41863.1"
                     /translation="MIVTTAQLFKVAYGNFAVGAYNINNMEQALGLFQGHIDAQAPFI
                     IQLSKGARKYANARVLEAIIRAVEAEYPDAIFAVHLDHGDEQTCYDCIDSGFYSSVMI
                     DASHAPFAENVATTRRVVERAHAKGVSVESELGMLGGVEEDIKVDEKHAMLTDPDEAR
                     AFVEQSGCDSLAVAIGTSHGAYKFSGSQGLHFDRIEAIQAALPGFPLVMHGSSSVPQD
                     EVLRINAAGGALNPSARGVDDNQFARAAKLGVTKVNIDTDGRLVWTRVHREFFRDHPE
                     GFDFRTPGKEYVRAYADFIVAKSRKLGSYGQLDKVRAAVS"
     gene            2515..3216
                     /gene="pdeM"
                     /locus_tag="PK829_11415"
     CDS             2515..3216
                     /gene="pdeM"
                     /locus_tag="PK829_11415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HOU41864.1"
                     /translation="MIETQVQGETLWLLPERAVYWPRAGALIVADVHFGKAAAFRAGG
                     IPVPGGTTAAMLARLSAALDRTAAARLLILGDLLHARAGRVPGTLAQVAAWRAARPDL
                     PITLIRGNHDSRAGDPPGDWDVECLDAPVAEAPFVWLHEPPSRSAGCQPALQAEEAGC
                     QPAPQGYPVAGHVHPAVTLSGGGRGLTLPCFYFGRDYALLPAFGEFTGTAVVRPRSGE
                     RVFVLAGGEIIAKPI"
BASE COUNT          531 a         1161 c         1027 g          505 t
ORIGIN      
        1 cgccgaacta caactggaca acaaccagta cttcctgaac gtgcccgtcg cggccgatct
       61 ggcggtcgat ccactctact ttggctcccg gccgggcagc gccaacgcgg tcgtcctgga
      121 agccaccgtc ttcaacctca gcgccgtggc ggcggccaac gtcccggtgg ccttctacaa
      181 caccaatggc cagcagctgg tcgtggtggc gaccgccacc atcagcagcc tgccggccgg
      241 cggcagcgcc atcgccaccg gcacgtgggc caacccgccg gcgcagtacg cggcgatggt
      301 ggtggtcgac ccggcgggtc aggttcccga ctcgaaccgg gagaacaacg ccgcctccct
      361 gacgcccggc ttcgaggtcg tgggcaaagc ggccaccgtc accgccgtcg ttggcacgag
      421 cgtgacgttc ccggtcgagg ttcgcagcgt cggcgggtat agcaactcgg tcgctttgga
      481 agcgctagac ctgccgcccg gcggcacggc cacggtcacg ccagcgagcg tcgccccgcc
      541 gggcacggcc acggtcgaga tcaccatccc tgccgacgcg cccccggccg tctatcccgt
      601 tcgggtggtg ggcgtgtccg gggagcgctc ggcgacggcc atcgtcaacg tgaacgtcgt
      661 gctgacgctg atgtgctatc cgctgaccgt aaccgtccag ggccagggcg cgccgcccac
      721 gctgtcgcct gagcgttcac ccgactgccc cggcgcgggc gactacgtcg ccggtcaggt
      781 aatcgacctg acagcccaac cggccgccgg ctggcgcgta agcgcctgga caggaacgct
      841 ggacgacgcc agtcaatcgc cggccaacgc cgcgatcatg ccggaatcca accacacggt
      901 gggcgtggtc tacgcgccgc tctgctactc gctgacgctg cagaaggagg gcgaaggcgc
      961 tttgcccgtg gccgcgccca acaagtcgag cgagtgcgcg gccaacggcc agtataagcc
     1021 gggtgagctg atcacgctga cagccagccc tgcggcgacg tggcgggtcg ccggttggcg
     1081 cggaaccggc aacgacgggg cgagcgccag cacgaaccag ttgaccatgc ccgcggccga
     1141 tcacgtggcc ggagtcgtat acgagcgaaa cgtgcgcctg ctttggctgc cggtgttgct
     1201 ggggccttaa cctaggtctc aaagacctga caggtaggcg gtcggcagca cttcagtgac
     1261 cgcgagcggg ccgctcacct gtcaggtttg caagacaacc tgcccccatt tcgtctgcct
     1321 gctataattc cccctgagcg tggcccggcg gcacagcttt ttccctgcgc ccctgccccg
     1381 ctccccgata gcccataacc aagactcaac caggagcacc tgcccatgat cgtcaccacc
     1441 gcccaactct tcaaagtcgc ctatggcaat ttcgccgtcg gcgcttacaa catcaataac
     1501 atggagcagg ccctcggcct cttccagggc cacatcgacg cccaggcccc gttcatcatc
     1561 cagctcagca agggcgcgcg caaatatgcc aacgcccgcg tgctggaagc catcatccgc
     1621 gccgtggagg ccgagtaccc cgacgccatc ttcgccgtcc atctcgacca cggcgacgag
     1681 caaacctgct acgattgcat cgactccggc ttctatagct cggtcatgat cgacgccagc
     1741 cacgcgccct tcgccgagaa cgtggccacc acccgccgcg ttgtcgagcg cgcccacgcc
     1801 aaaggcgtca gcgtcgagtc ggagctgggc atgctcggtg gcgtggagga ggacatcaag
     1861 gtcgatgaga agcacgccat gctgaccgac cccgacgagg ccagggcgtt cgtggaacag
     1921 tccggctgcg acagcctggc cgtggccatc ggcacgagcc acggggccta taagttctct
     1981 ggttcgcagg ggcttcattt cgaccgcatt gaggccattc aggcggcgct gcccggcttc
     2041 ccgctggtca tgcacggcag cagcagcgtg ccccaggacg aggtgctgcg catcaacgcg
     2101 gccggcgggg cgctcaaccc atcggccagg ggcgtcgacg acaaccagtt cgcccgcgcc
     2161 gccaagctgg gcgtgaccaa ggtcaacatc gacaccgacg gccgcctcgt ctggacgcgc
     2221 gtccaccgcg agttcttccg cgaccacccg gagggcttcg acttccgcac gccgggcaag
     2281 gaatacgtcc gggcctacgc cgacttcatc gtcgctaaga gccgcaagct cggctcctat
     2341 ggccagctgg acaaggtgcg cgccgccgtt tcctgatcga agaacctcac gcaaaggacg
     2401 caaagggggc aaagaacgca aagaagacaa ggagattctc ttcttggcgt cctttgcttc
     2461 cctggcgtcc tttgcgtgag ctcttcttag cgcctttgcg tgagcttctc tcccatgatc
     2521 gagacacaag tgcagggcga gacgttgtgg ctgctgccgg agcgggccgt ctattggccg
     2581 cgggcggggg cgctgatcgt ggccgacgtc cacttcggca aagcggccgc ctttcgcgcc
     2641 ggcggcattc ctgtgcccgg cggcacgacg gccgccatgc tggcccgcct cagcgccgcc
     2701 ctcgaccgca cggccgcggc gcggctgctc atcctgggcg acctgctgca cgcccgcgcc
     2761 ggccgcgtcc cgggcacatt ggcgcaggtg gctgcctggc gcgccgcccg ccccgacctg
     2821 cccatcaccc tcattcgcgg caaccacgac agccgcgccg gcgacccacc gggcgattgg
     2881 gacgtggagt gcctggacgc gcccgtggcc gaagcgccgt ttgtctggct ccacgagcca
     2941 ccaagtcgta gcgcaggttg tcaacctgcg ttacaggcgg aagaggcggg ttgccaaccc
     3001 gccccacaag ggtaccctgt cgctggccac gtccacccgg ccgtgaccct cagcggcggc
     3061 ggccgcggcc tgacgctgcc gtgcttttac ttcggccgcg actatgctct gctgccggcc
     3121 tttggcgagt tcacgggcac ggccgttgtc cggccgcggt cgggggagcg cgtcttcgtc
     3181 ctggccggcg gcgagatcat cgccaagccg atctagagcc tgat
//
LOCUS       DAOSMQ010000374         3222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_29948_length_3222_cov_4.845911, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000374 DAOSMQ010000000
VERSION     DAOSMQ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29948_length_3222_cov_4.845911"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PK829_11420"
     CDS             <1..583
                     /locus_tag="PK829_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41865.1"
                     /translation="ILAAQRFGAQPSGGPGGTFLASWRHNGGVASDLARLDETRAAWL
                     RALPAMALDGDWLLAHADATFYQVYGRTVGAVNRGIRAILHDDNAADWDLLLDYFSQR
                     RAFQDEERGPARVARFLSLYGGRRFVHGHTPIVKLNGRQPEDVTAAHVYAGGLAVDVD
                     AGLYLGGPGFVTILSDNGAGGNYRDQPQGEWRI"
     gene            580..1107
                     /locus_tag="PK829_11425"
     CDS             580..1107
                     /locus_tag="PK829_11425"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOU41866.1"
                     /translation="MIDNSNSTQETHSYSEAEMYRRSLKGRADAKRTFAEVVADKLTI
                     AFGSMVFLVVNVIWFAVWIVINVGLIPGVEPFDPFPFGFLTMVVSLEAIALAIIVLIS
                     QNRAAKIADLREEVDLHIDMLAEAELTKLLQLVTRLAEQQGINLSDDSELQEMLEPSD
                     TEQLQQALEDDISNS"
     gene            1142..1900
                     /locus_tag="PK829_11430"
     CDS             1142..1900
                     /locus_tag="PK829_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU41867.1"
                     /translation="MRIAIFSDVHGNLTALEAVLADIDRHAPDAIVFAGDLCLFGPRP
                     AECLRLVRDRRFPAVVGNTDAWLAGRAQPPDKHAATMAWTQARFLPDDTAWLGRLPFG
                     LRFAPTAEADDDLLIVHANPRNWDDIVFPAEADQLAQWGHVRQPDAELEPLFSGLTAA
                     VVAYGHLHIPGVRPWGGLTLLNVSSVSMPGDGDGRAKYALVEWQAGRWSATHHRVAYD
                     VGAEADAFRAHQPPRWQEALAAIEADGYYYPQKI"
     gene            2034..2522
                     /locus_tag="PK829_11435"
     CDS             2034..2522
                     /locus_tag="PK829_11435"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU41868.1"
                     /translation="MTATDKHAIKEELRAAREELYRVVSGLSSAQWQQRAYSEEGGEW
                     RVLDLLRHVVDSERGMTALMAQVQAGGAGVSDDFDLSRWNRRVVEKLQDKTLDDLMAA
                     MAQNRVALFDFIDTLADEDWSQKGRHASGRILTIEQVCELIASHERAHTAEILAATAA
                     TA"
     gene            complement(2574..3089)
                     /locus_tag="PK829_11440"
     CDS             complement(2574..3089)
                     /locus_tag="PK829_11440"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOU41869.1"
                     /translation="MRCGIYLQTFAESNSRLGFIPGSHRPPTGPVSGSTKRKERWLKW
                     LGAANYLSPKIQMAISNADWIPTEPGDCVIFDPRTIHSGSYITGPKYSVFVAYGVENK
                     HFSNHYNYYRNVRSELGYGDMEPEFVQLLREHDLYPEETPTFDFVEGAWVPSSLVKSL
                     YTRRRDTPVQM"
BASE COUNT          555 a         1096 c         1051 g          520 t
ORIGIN      
        1 catcctggcc gcgcagcgct ttggcgccca gcccagcggc ggccccggcg gcaccttcct
       61 ggccagctgg cggcacaacg gcggcgtggc gtccgacctg gcccggctgg acgagacccg
      121 cgccgcctgg ctgcgcgccc tgccggcgat ggctctcgac ggcgactggc tgctggccca
      181 cgccgacgcg actttctacc aggtctacgg ccgcaccgtc ggcgcggtca accgcggcat
      241 ccgcgccatc ctgcacgacg acaacgccgc cgactgggat ctgctgctcg actacttctc
      301 gcagcggcgc gccttccagg acgaggagcg cggcccggcc cgcgtggccc gcttcctgag
      361 cctctacggc ggccgccgct ttgtccacgg ccacacgccc atcgtcaagc tcaacggccg
      421 ccagccggag gacgtcaccg ccgcccacgt ctacgccggc ggcctggccg tcgatgtcga
      481 tgccgggctg tacctgggtg gccccggctt cgtaacgatc ctgtcggata acggggctgg
      541 cgggaactac cgcgatcaac cgcaaggaga gtggcgaata tgatcgacaa cagcaacagc
      601 acccaggaga cgcactccta ctccgaagcg gagatgtacc gccgcagcct gaagggccgc
      661 gccgacgcca agcgcacctt cgccgaagtc gtcgccgaca agctgaccat cgccttcggc
      721 agtatggtct tcctggtggt caacgtcatc tggttcgccg tctggatcgt catcaacgtc
      781 ggcctgattc ccggcgtcga gccgttcgat cccttcccct tcggctttct aacgatggtc
      841 gtctcgctgg aagccatcgc cctggccatc atcgtcctca tctcccagaa ccgcgccgcc
      901 aagatcgccg acctgcgcga ggaagttgac ctccacatcg acatgctggc cgaggcggaa
      961 ctgaccaagc tgctgcaact ggtcacccgg ctggccgagc agcaaggcat caacctcagc
     1021 gacgacagcg agttgcagga gatgctcgaa ccgtcggaca cagagcagct tcagcaggcg
     1081 ctggaagacg acatcagcaa cagctagagt tacatctctg gaggggctat ctggagtggc
     1141 tatgcgaatc gcgatcttct ccgatgttca tggcaacctg accgccctgg aggccgtcct
     1201 ggccgacatc gaccggcacg cgcccgacgc catcgttttc gccggcgacc tgtgcctgtt
     1261 cggtccccgg ccggccgagt gcctgcgcct ggtgcgcgac cggcgcttcc cggctgtcgt
     1321 cggcaacacc gacgcctggc tggccggccg cgcccagcca cccgacaagc acgccgcaac
     1381 gatggcctgg acacaggcgc gcttcctgcc ggacgacacg gcctggctgg gccggctgcc
     1441 gttcggcctg cgcttcgcgc ccacggccga ggccgacgac gacctgctca tcgtccacgc
     1501 caacccgcgc aactgggacg acatcgtttt cccggccgag gctgaccagt tggcgcagtg
     1561 gggccacgtg cggcagcccg acgccgagct ggagccgctc ttcagcgggc tgacggccgc
     1621 cgtcgtcgcc tatggtcatc tgcacatccc cggcgtgcgc ccctggggcg ggctgacgct
     1681 gctcaacgtc agctcagtca gcatgcccgg cgacggtgac gggcgggcca agtatgccct
     1741 ggtggagtgg caggccggcc gctggtcggc tacccaccac cgcgtggcct acgatgtcgg
     1801 ggccgaggcc gacgcctttc gcgcccacca gccgccccgc tggcaggaag cgctggcggc
     1861 gattgaggcc gatggatact attatccgca gaagatatag gggatagggg ctaggggcta
     1921 ggggctaggg gctaggttcc agggatcagg ttccgtaaga ctagccgagg actgacccac
     1981 tgacttattg acccattgac ccactgacct actgaccaca tggagaacgt aatatgaccg
     2041 caaccgacaa acacgcaatc aaggaagagc tgcgggcggc gcgggaggaa ctataccgcg
     2101 tcgtgtccgg gctgagcagt gcccagtggc agcagcgcgc ctattccgag gagggcggcg
     2161 agtggcgcgt gctcgatctc ctgcgtcacg tcgtcgattc ggagcgcggc atgacggcgc
     2221 tgatggccca ggtgcaggcc ggcggcgcgg gcgtgtccga cgatttcgac ctgtcccgtt
     2281 ggaatcgccg cgtcgtcgag aagctgcaag acaagacgct cgacgacctg atggccgcta
     2341 tggcccagaa ccgcgtggcc ctatttgact tcatcgatac gctggcggac gaggattgga
     2401 gccagaaggg ccgccacgcc agcggacgca ttctgaccat tgagcaggtc tgcgagctga
     2461 tcgccagcca cgagcgcgcc cacacggccg agattctggc ggcgacggcc gcgacggcct
     2521 aaaccaaaaa gggccggggc gaagtgagtc cgccccggcc ccgcgccgca gccctacatc
     2581 tgcactggcg tgtcgcgccg ccgcgtgtag aggctcttga ccagcgacga cggcacccac
     2641 gctccctcaa cgaagtcgaa ggtcggcgtc tcctccgggt agaggtcgtg ctcgcgcaag
     2701 agctggacga attcaggctc catgtcgcca taacccaact cggagcgcac gttgcggtag
     2761 tagttgtagt ggttggagaa gtgcttgttc tcgacaccgt aggcgacgaa gacagagtac
     2821 ttcggcccgg tgatgtaact gccggagtgg atcgtgcgcg ggtcgaagat gacgcaatcg
     2881 cccggctcgg tcggaatcca gtcggcgttg gagatggcca tctgaatctt gggcgacagg
     2941 tagttggccg cgcccagcca cttcagccag cgctccttgc gcttcgttga acccgacacc
     3001 ggccccgtgg gcggcctgtg gctgccgggg atgaagccga ggcgcgagtt gctctcggcg
     3061 aacgtttgca ggtagatgcc gcagcgcacc agcttgtagg gttccgcgtc tcgtcccagt
     3121 ccggcccaac gccatactcc cggttaacgc tgtcgcgatg ccaggagatg gccgagaagc
     3181 cgacgtggat gtcgttgtgc tgcaagaagc gcacgttggg at
//
LOCUS       DAOSMQ010000375         3212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30051_length_3212_cov_5.671524, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000375 DAOSMQ010000000
VERSION     DAOSMQ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30051_length_3212_cov_5.671524"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PK829_11445"
     CDS             <1..1184
                     /locus_tag="PK829_11445"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOU41870.1"
                     /translation="FRAARPLGHDEPLDVPVPAPPPPPRATLIATDGSQILPDRHAPF
                     LYYLINIGAITYYHGEPAAPGIVSLPSIFYPTIEDDLTDDSPGFNKSEVTIARDRREI
                     ETLAALALSMQGQAARTLALLDQRLLYWPFVGEREKTEEAVFAWTAAMTQIYETGALL
                     AGYIDRPGKASVVTLLQTLLDDDTINLKELGRRPAAGELTDADLYATLLGPGERSPVF
                     VDVSPANSRFAEENPLIEVCFFYLNPGSRGQVPGSREEEASIGPEAAGDSSSPGTWPL
                     EPGPFPLEPGPWNLEPTLARAIARVDIPRWVAEDPAAVEFVHGLIYDQCKLMGDYPYV
                     LARADELAVVGRFDEMNLNMMIDGHMQRLGVPDGLLAAKADSKGIARGGRSHHEFRPG
                     L"
     gene            1268..1918
                     /locus_tag="PK829_11450"
     CDS             1268..1918
                     /locus_tag="PK829_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41871.1"
                     /translation="MELFMETTTTVGRVLRAGTQGFTIGCQVRQLSGPSFGCLVKAQP
                     ADTREAIFGLVYNMNVDDDPLVRRLILADSQPEAVINDQRQNRLLPIEMNVLAVGYCR
                     DGRFFHGLPPRPPLNLDPVVLCEESEIRRFTRQLGYLRLILRSADLGVPVDQLLVAHV
                     QDIYRLRHHDADWAMAAVREIIELLRNNYETLVPVLEALGEALPELDGVPGAAGGF"
     gene            1918..>3212
                     /locus_tag="PK829_11455"
     CDS             1918..>3212
                     /locus_tag="PK829_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOU41872.1"
                     /translation="MLPTLPDPTPQPTSDPLGYVVGGGLKEGFRIRLTVPADSVQEGS
                     FVVCESGHFRYYGLVTDLQLGSTDPRFADEQSDRLPVSLRQALYGKTLYTTLAVYPTL
                     MLDLGPDLTAPERAEWQARVDRGEENPGPRPVKTVPAHHAPVRPADAADVAAIFGEEG
                     GANFVVGHTIEQGYPVCLDLARFVKRSSGIFGATGTGKSFLTRMVLAGLMKADVAAAL
                     VFDMHNEYGYDDRDADRDTTVRGLKAMFGQKAQIAGLGKSTRVRGASPDFTLELSTRD
                     FGTADILLLSEALDLNETAAVTLSALERAFQRDWLAEFMALKPGAVVEDEAGKKVPAG
                     DSVAYWANGHNIHPAAAESLHRKLQPIFNRAYVVERPAANAVRAIVDQLENGRHVILS
                     FGEYDNPLDYMLVSNVLTRRIRQQWVDKTERHKSFGEAPP"
BASE COUNT          546 a         1173 c         1018 g          475 t
ORIGIN      
        1 actttcgcgc cgcccggccg ctgggccacg acgagccgct ggacgtgccc gtccccgccc
       61 cgccgccgcc gccgcgggcc acgctcatcg ccaccgacgg ctcgcagatc ctgcccgacc
      121 gccacgcgcc cttcctctac tatctgatca acatcggcgc tatcacctac taccacggcg
      181 aaccggctgc gcccggcatc gtctcgctgc cgtccatctt ctaccccacc atcgaggacg
      241 acctgaccga cgacagccct ggcttcaaca agagcgaagt caccatcgcc cgcgaccggc
      301 gcgagatcga gacgctggcc gcgttggccc tcagcatgca gggccaggcc gcgcgcacgc
      361 tggccctgct cgaccagcgc ttgctctact ggcccttcgt cggcgagcgc gagaagaccg
      421 aagaagctgt tttcgcctgg acggcggcca tgacccagat ctacgagacg ggcgcgctgc
      481 tggccggcta catcgaccgt ccaggcaaag cctcggtcgt cacgctgctg caaacgctgc
      541 tcgacgacga caccatcaac ctgaaggaac tgggccggcg gccggcggcc ggcgaactga
      601 ccgacgccga cctctacgcc acgctactcg gccccggcga gcgcagcccc gtcttcgtcg
      661 atgtctcccc ggccaacagc cgcttcgccg aggagaaccc gctcattgag gtgtgcttct
      721 tctacctgaa tccaggttcc aggggccagg ttccaggttc cagggaagag gaagcgagca
      781 taggcccaga ggctgcggga gacagctctt cccctggaac ctggcccctg gaacctggcc
      841 ccttccccct ggaacctggc ccctggaacc tggaacctac tctcgcccgc gccatcgccc
      901 gcgtggacat tccccgttgg gtggccgagg acccggcggc ggtggaattc gtccacgggc
      961 tgatctatga ccagtgcaag ctgatgggcg actatcctta tgtgctggcg cgggcggacg
     1021 agttagccgt cgtcggccgc ttcgacgaga tgaacctgaa catgatgatc gacggccaca
     1081 tgcaacggct gggcgtgccc gatgggctgc tggccgccaa ggccgacagc aagggcatcg
     1141 cccgcggcgg gcgcagccac catgaattcc ggccggggct ttaggagggt gacgacaacg
     1201 cggacgtagc atcgccgaga ggagcacccc gcccgcgcgg cagggaggtt tctcgaccga
     1261 ggcgcgcatg gagctattta tggagacgac aacgacagtc gggcgggtct tgcgggcggg
     1321 cacacagggc ttcaccatcg gctgccaggt acggcagttg tccgggccat cgttcggctg
     1381 cctggtcaag gcccagccgg ctgacacgcg cgaggccatc tttggcctgg tctacaacat
     1441 gaacgtcgat gacgacccac tcgtgcgccg gctcatcctg gccgacagcc agccggaggc
     1501 ggtcatcaac gaccagcgcc agaaccggct gctgcccatc gagatgaacg tcctggccgt
     1561 cggctattgc cgcgacggcc gcttcttcca cggcctgcca ccccggccac cgctcaacct
     1621 tgacccggtg gtgctgtgcg aagagagcga gatcagacgc ttcacccggc agctgggtta
     1681 cctgcggctc atcctgcgca gcgccgacct gggcgtgccc gtcgaccagc tccttgtggc
     1741 ccacgtgcag gacatctacc gcctgcgcca ccacgacgcc gactgggcca tggccgccgt
     1801 gcgcgagatc atcgaactgc tgcgcaacaa ctacgagacg ctcgtgcccg tgctggaagc
     1861 cctcggcgaa gcgctgccgg aactggatgg cgtgcccggc gcggcgggag gcttctgatg
     1921 ttgcccacgt tgcccgaccc caccccccaa cccaccagcg acccgctcgg ctacgtcgtc
     1981 ggcggcggcc taaaggaggg cttccgcatc cgtctgaccg tgccggccga cagcgtgcag
     2041 gagggcagct tcgtcgtctg cgagagcggc cacttccgct actatggcct ggtgaccgac
     2101 ctgcaactgg gcagcaccga tccccgcttc gccgacgagc aatccgaccg gctgccggtc
     2161 agcctgcgtc aggcgctcta cggcaagaca ctctacacga ccctggccgt ctaccccacg
     2221 ctgatgctcg acctggggcc agacctgacc gcgccggagc gggccgagtg gcaggcgcgc
     2281 gtcgaccgcg gcgaagagaa ccccggcccg cgaccggtga agaccgtgcc cgcccaccac
     2341 gcgccggtgc gcccggccga cgccgccgac gtggccgcca tcttcggcga ggaaggcggg
     2401 gccaacttcg tcgtcggcca caccatcgag cagggctacc ccgtctgcct cgacctggcc
     2461 cgcttcgtta agcgcagcag cggcatcttc ggcgcgacgg gcacgggcaa gagcttcctg
     2521 acgcgcatgg tgctggccgg gctgatgaag gccgacgtgg ccgcggccct ggtcttcgac
     2581 atgcacaacg agtacggcta cgacgaccgc gacgccgacc gcgacacgac cgtccgcggt
     2641 ctgaaagcca tgttcggcca gaaggcgcag atcgccgggc tgggcaagag cacgcgcgtc
     2701 cgcggcgcgt cgcccgactt caccctggag ctttcgacgc gcgacttcgg cacggccgac
     2761 atcctgctgc tgtccgaggc gctggacttg aacgagacgg cggcggtgac gctcagcgcc
     2821 ctggagcgcg ccttccaacg cgactggctg gccgagttca tggccctgaa accgggcgca
     2881 gtggtcgagg acgaggccgg caaaaaggtt ccggccggcg attccgtcgc ctactgggcc
     2941 aatggccaca acatccaccc cgccgcggct gaatcgctgc accgcaagct gcaacccatc
     3001 ttcaaccgcg cctacgtcgt cgagcggccg gcggccaacg ctgtcagggc catcgtcgat
     3061 cagttggaga acggccgcca cgtcatcctc agcttcggcg agtacgataa cccgctggac
     3121 tacatgctcg tctccaacgt cctgacgcgg cgcatccgcc agcagtgggt cgataagacc
     3181 gagcgccata agagcttcgg cgaggctccg cc
//
LOCUS       DAOSMQ010000376         3212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30053_length_3212_cov_4.584732, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000376 DAOSMQ010000000
VERSION     DAOSMQ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30053_length_3212_cov_4.584732"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK829_11460"
     CDS             complement(<1..284)
                     /locus_tag="PK829_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41873.1"
                     /translation="MNRKFRHLLLLLALLGALPAVATTAATVHGQTEPSISTFLPLVD
                     VGDDWQASEYATDIAFGDVDGDGRDEMAIVRNAVTGPRVLLLDDAAAGFAP"
     gene            complement(295..2319)
                     /locus_tag="PK829_11465"
     CDS             complement(295..2319)
                     /locus_tag="PK829_11465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU41874.1"
                     /translation="MTDNEAAAPRPTQADDLYRFQLPSDPQIAPDGTQVVFCVQRVER
                     ETEKKYTNLWLADSDGGRLRQFTHGKWADTQPRWSPDGRTLAFLSNRGSEEQAQLYLL
                     PTDGGEARPLTDLKGNFANYEWSPDGARLVCQFRQKDADALEREKDEAKKKLGLSYRH
                     ITRADYKFDGAGYLPQERWHVWVIDATTGTATQLTEGSHDETEPRWSPDGQSILFASN
                     RSDRPDMDLDATELYLIPAGGGAMRQLATRPGRKFAAVFSPDGQYVAYLGRDKPGRWY
                     QNARLYVAPLAGGGAREVGGELDLHLSGATNGDVFNDSTPSLPVWSADGRRVACVATT
                     RGNEWLLSLNVFEPNDAPQWLIAEAGVLGGYTLDRARQRVAYLWSTLDDPAQLVVRDL
                     AYDAPAGPRTLTALNPWLAEKEMGTLEEVWFKAYDGHDLHGWILKPPGFDPSRRYPSI
                     LQIHGGPQTQYGNIYMHEFHYLAAAGYVVYWSNPRGSQGYGEAVTGAIYGQWGTVDYD
                     DVMAWADYVEKLPYIDPERMGVTGGSYGGYMTTLIIGRTQRFKAAVAQRVVSNFLSFY
                     GSSDLNAGLEHLAGTAAPWDDVQTNWAQSPISAIGNARTPTLVIHSENDHRCDQEQGE
                     QVFIALRRLGVATELLLLPGESHGVSRGGRTDRRVARLEYMLGWFERYLK"
     gene            complement(2427..>3212)
                     /locus_tag="PK829_11470"
     CDS             complement(2427..>3212)
                     /locus_tag="PK829_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU41875.1"
                     /translation="VAAADGVIVGRHEGYPDQSCSPNGNRWNAVYVRHADGSIAWYGH
                     LKLGSVTTKPVGATVVRGEVLGLVGSSGNSSGPHLHFEMYNGGALLDPYAGSCNALDG
                     GWWEEQRAYNDAAINKLTTGTAPVERSACPAPDIPHEATLFQPGDRVTFTAYYHDQLD
                     SLPGVYRILTPGGAVFAQWTHSSTRPHSTLSYWYWAYVIPATAQTGTWRFEVDFNGRA
                     YSHAFVVGVPSTPTATPTATPTATPTRPWRPSDFIFLPSVVKE"
BASE COUNT          486 a         1039 c         1140 g          547 t
ORIGIN      
        1 ggcgcaaagc cggcggcggc gtcatcgagc agcagcaccc gcggcccggt gacggcgttg
       61 cggacgatgg ccatctcgtc gcggccgtcg ccgtctacgt caccaaaagc gatgtcggtg
      121 gcgtactccg acgcctgcca atcgtcgccg acgtcgacca ggggcaggaa agtcgagatg
      181 gacggctcgg tctggccgtg gacagtggcc gcggtcgtgg caacggccgg cagcgccccc
      241 agcagggcca acaacaggag cagatgacgg aacttccggt tcatgacttt tccctcactt
      301 cagatagcgc tcaaaccagc ccaacatata ttccagccgg gcgacgcgcc ggtctgtccg
      361 gccgccgcgg ctgacgccgt gggactcgcc cggcaatagc aacaactccg tagcgacacc
      421 cagccggcgc agggcgatga acacctgctc gccctgctcc tggtcgcaac ggtggtcgtt
      481 ctcgctgtgg atgaccagcg tcggcgtgcg ggcgttgccg atggcgctga tgggcgactg
      541 cgcccagttg gtctggacgt cgtcccacgg cgcggccgtg ccggccaggt gctccagtcc
      601 ggcgttcaga tcgctggagc cgtagaagct gaggaagttg ctgacgacgc gctgggccac
      661 ggcggccttg aagcgctggg tgcggccgat gatcagcgtg gtcatgtagc cgccatagct
      721 gccgccggtg acgcccatgc gctccgggtc gatgtagggc agcttttcaa catagtcggc
      781 ccaggccatg acgtcgtcgt aatcgaccgt gccccactgg ccatagatgg ccccggtaac
      841 ggcctcgccg tagccctggc tgccgcgcgg gttgctccaa tagaccacgt agccggcggc
      901 ggccagatag tggaactcgt gcatgtagat gttgccgtac tgcgtctgcg gtccgccgtg
      961 aatctgcagg atcgacgggt agcgccgtga gggatcgaac ccgggcggct tgagaatcca
     1021 gccgtgcagg tcgtggccgt catatgcctt gaaccacacc tcttcgagag tgcccatctc
     1081 tttctccgcc agccacgggt tgagcgccgt cagcgttcgc ggcccggcgg gggcgtcgta
     1141 agccaggtcg cggacgacca gttgcgccgg atcgtcgagc gttgaccaca ggtaggccac
     1201 gcgctgccgc gcccggtcga gggtgtagcc gcccagcacg cctgcctcgg cgatgagcca
     1261 ctgcggcgcg tcgttcggct caaagacgtt cagcgagagt agccactcgt tgccgcgcgt
     1321 cgtcgccacg caagccacgc gccggccgtc ggccgaccag accggcagcg acggcgtcga
     1381 gtcgttgaac acgtcgccgt tcgtcgcccc ggacaggtgg aggtccagct cgccgcccac
     1441 ctcgcgcgcg ccgccgccgg ccagcggggc cacgtagagc cgcgcattct gataccagcg
     1501 gccgggcttg tcgcgcccca gataggccac gtactgccca tcgggcgaaa agacggcggc
     1561 gaacttgcgg ccgggccgcg tcgccagttg tctcatcgcg ccgcctccgg ccggaatcag
     1621 gtacagttcc gtcgcgtcca ggtccatgtc gggccggtcg ctgcggttgg aggcaaagag
     1681 gatgctctgc ccgtcgggcg accagcgcgg ttccgtctcg tcgtggctgc cttcggtcag
     1741 ttgcgtggcc gtgcccgtcg tggcgtcgat gacccagacg tgccagcgct cctggggcag
     1801 atagcccgcg ccgtcgaatt tatagtcggc gcgggtgatg tggcgatagg acaaacccag
     1861 cttcttcttg gcctcgtcct tttcccgctc cagtgcgtcg gcatccttct ggcgaaactg
     1921 gcagacgagg cgcgcgccat cgggcgacca ttcgtagttg gcaaaattgc ccttcaggtc
     1981 ggtcagcggc cgggcctcgc cgccgtcggt cggcagcagg tagagctgcg cctgctcctc
     2041 gctgccacgg ttggacagaa aggccagcgt ccggccgtcg ggcgaccagc gcggctgcgt
     2101 gtcggcccac ttgccgtggg tgaactggcg caggcggccg ccgtcgctgt cggccagcca
     2161 caaattggtg tatttcttct ccgtctcgcg ctccacgcgc tggacgcaga agactacctg
     2221 cgtgccgtcg ggcgcaatct gcgggtcgga cggcaactgg aaccggtaca ggtcgtcggc
     2281 ttgggtcggc cgcggcgcgg cggcctcgtt gtcggtcatc tcttcactcc gtgtgggtag
     2341 gctatgggct aacgatacca cattgcgggc tgcgaggaga agacgatacg tggggcgcgt
     2401 ggcccgttcc cgaatggtcg cgcagcctac tctttgacca cggagggtag aaagatgaag
     2461 tcgctgggcc gccaggggcg ggtgggggtg gctgttggcg tggctgtggg ggtggccgtg
     2521 ggggtggagg ggacgccgac gacgaaggcg tggctatagg cgcggccgtt gaaatccacc
     2581 tcgaatcgcc acgtgcccgt ctgggccgtc gccgggatga cgtaggccca ataccagtag
     2641 gacaacgtgg aatggggccg cgtgctgctg tgggtccact gggcgaagac cgcgccgccg
     2701 ggcgtcagga tgcggtagac accgggcagg ctgtcgagtt ggtcgtggta gtaggccgtg
     2761 aaggtgacgc ggtcgccggg ctggaagagc gtcgcctcat gggggatgtc cggcgcgggg
     2821 caggccgagc gctccactgg cgctgtgccc gtggtcagct tgttgatggc tgcgtcgttg
     2881 taagcccgct gctcctccca ccagccgccg tcgagagcgt tgcagctgcc cgcgtagggg
     2941 tcgagcagcg cgccgccgtt gtacatctca aaatgcaggt gcggcccgct ggagttgccg
     3001 ctgctgccca ccaggcccaa cacctcgccg cgaacgaccg tcgcgccgac gggcttggtg
     3061 gtgacggagc caagtttaag gtggccatac caggcgatgg agccgtcggc gtggcgcacg
     3121 tagacagcgt tccagcggtt gccgttgggg ctgcaactct ggtcggggta gccctcatgc
     3181 cggccgacga tgacgccatc ggccgcggcc ac
//
LOCUS       DAOSMQ010000377         3197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30222_length_3197_cov_3.935710, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000377 DAOSMQ010000000
VERSION     DAOSMQ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30222_length_3197_cov_3.935710"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK829_11475"
     CDS             <1..308
                     /locus_tag="PK829_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41876.1"
                     /translation="TTALYDAGFVLLNRYVVHAEHPISVHIRNTHALTHDAILVLSPG
                     APGDGPAWATPGLPRPDGADFTADCAALLGWVLTQPGLGAEAIGRLWQASLAGAAAG"
     gene            complement(305..1132)
                     /locus_tag="PK829_11480"
     CDS             complement(305..1132)
                     /locus_tag="PK829_11480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41877.1"
                     /translation="MMRRQGQSGNTRRYLRTHWPVFFGLYSVLVLTVVLLGVGLASGW
                     YALVPFAVAVMLVAGYVLVAALWTAYQTYDGPGPLVMTQLIDYAQIRPDDKVVCIDLG
                     TRATAVEIARRLVTGQVTAIDVYNPQSNPGAPLRRARAAARKPGPDPRLEWIDGSIAL
                     LPMPDHSVRLVFMNEVLSEFWLPEERAQLLEEVRRILVPEGRLLLAERHRTRSNLLLS
                     GLATSSLPPAETWHALLLRADFRIQREDSLRGLVYCARADRPPPTEGRQLHLHLEFL"
     gene            1310..1927
                     /locus_tag="PK829_11485"
     CDS             1310..1927
                     /locus_tag="PK829_11485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41878.1"
                     /translation="MDELDDTRQRFTERYEEGHVPWDDPLPPPEILELAETRPPGRAL
                     DVGCGFGRAAIYLAQRGWTVVGVDFIPQAIDEARRRAADAGVAERTRFVVASATDLTF
                     LAPPFDLVIDVGCAHSFADDMLRRYSDELARLLAPGGEYILHARLRDSEAAGEERPHG
                     LEVATLLALLADDFVLERQEYGTTRVEDRPPWNSAWFWFRRAREE"
     gene            complement(1956..3101)
                     /locus_tag="PK829_11490"
     CDS             complement(1956..3101)
                     /locus_tag="PK829_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HOU41879.1"
                     /translation="MTGRILLLSPYHGGSHRAWAEGYACASAHAIALLTLPAHFWKWR
                     MHGGAVTLARQWLSQAASQPLPDLILATDLFDLTTFLALTRRHTAGIPAALYMHENQL
                     TYPLPDDPATGPMRRQLGERDRHYAFINYASMLAADRVFFNSRYHLETFFAALPAFLR
                     HYPEYNELDTILLLRRRSAVLPVGIDLRRLEPPETAAAGAPPLILWNQRLEFDKRPER
                     FMDVLLTLAGEGLPFRVALCGERFGQPDAAWLAGVEALGERVIHNRYANDDTYRHLLW
                     QAAATFSTADHEYFGIGVLEAIYAHTFPLLPDRLSYPEIIPDAFHADCLYHGKADLLA
                     RLRGVLANPAAARARATALASAIAGYDWAVMAPTYDRAFDGLMAQRG"
BASE COUNT          556 a          998 c         1129 g          514 t
ORIGIN      
        1 tgaccacggc gctctatgac gctggctttg tcctactcaa ccgttacgtc gtccacgctg
       61 agcaccctat ctccgtccac atccgcaaca cccacgcgct gacccacgac gccatcctgg
      121 tgctgtcccc cggcgcgccc ggtgacggcc cggcgtgggc gacaccaggg ttgccgcgcc
      181 ccgatggcgc ggacttcacc gccgattgcg ccgcgttgct cgggtgggtg ctgacacagc
      241 cgggactggg cgcagaggcg attggccggc tgtggcaggc gtcgctggcc ggggcggctg
      301 cgggctaaag gaactccagg tggaggtgta gttgccggcc ctcggtcggc ggtggccggt
      361 cggcgcgggc gcagtagacc aggccgcgca ggctatcctc gcgttggatg cggaagtcgg
      421 cccgcaagag cagggcgtgc cacgtctccg ccggcggcaa cgacgatgtc gccagaccgc
      481 tgagcagtag attgctgcgc gtgcggtggc gctcggccag cagcagccgg ccttccggca
      541 cgaggatgcg ccgcacctct tccagtagtt gggcgcgttc ctccggcagc cagaactccg
      601 acagcacctc attcatgaag accagccgca cgctgtgatc gggcatgggc aggagtgcga
      661 tgctgccgtc gatccattcc aggcgcgggt cagggccggg cttgcgggcg gcggcgcggg
      721 cgcggcgcaa aggcgcgccg gggttggact gaggattgta gacatcgatg gccgtgacct
      781 ggccagtgac gagccgccgg gcaatctcga cagccgtagc gcgcgtgccg aggtcgatgc
      841 aaacgacctt atcatcgggg cgtatttgcg cgtagtcaat tagctgagtc ataaccagtg
      901 ggccggggcc gtcgtaggtc tggtaggccg tccacagcgc ggccacgagc acgtagccgg
      961 ccacgagcat gactgccacg gcaaagggga cgagcgcgta ccagccgctg gccaggccaa
     1021 caccgagcag cacgaccgtc agcaccagca cgctgtatag cccgaaaaag accggccaat
     1081 gggtgcggag atagcggcgc gtgttgccag actggccctg tcgtctcatc atgtcgcgtg
     1141 ctcagcagtc gccctattcc agggcttttc cagagtcacc cccggcgcac cggtcaggtc
     1201 aatggtcgct agacgacttt ggaaaagccc tgcatcctat tcgcagttac agggaatgat
     1261 actattgtaa gggacaacca ggagaggcga caaccgccag gagcagacaa tggacgagtt
     1321 agacgacacc cggcaacgct ttacggagcg ctatgaagag gggcacgtgc cctgggacga
     1381 cccgctgccg ccgccggaga ttcttgagct ggccgaaaca cggccgcccg gccgcgcgct
     1441 cgacgtcggc tgcggctttg ggcgcgcggc catctacctg gcccaacgag gctggacggt
     1501 cgtcggcgtg gacttcatcc cccaggccat cgacgaggcg cggcggcggg cagctgacgc
     1561 gggcgtggcc gagcggacac gctttgtcgt cgcctcggcc accgacctga ccttcctcgc
     1621 accgccgttc gatctagtca tcgacgtcgg ctgcgcccac tcctttgccg acgacatgct
     1681 gcgacgctat agcgacgagt tggcccggct gttggccccc ggcggcgaat acattctcca
     1741 tgctcgtctg cgcgacagcg aggcggccgg cgaggagcgg ccgcacggcc tcgaagttgc
     1801 taccttgctg gcgctgttgg ccgatgactt tgtgctggag cgccaggaat atggcacgac
     1861 acgggtagag gatcggccgc cgtggaactc ggcctggttc tggttccggc gggcgaggga
     1921 agaatagggc gctgctcgcg tcaccagcct gtcatttacc cgcgttgcgc catcaagccg
     1981 tcaaaggcgc ggtcataggt gggagccatc accgcccagt catagccggc aatggcgctg
     2041 gccaacgcag tggcgcgggc gcgggcggcg gccgggttgg ccagcacgcc gcgcagccgg
     2101 gccagcaggt cggccttgcc gtggtagagg cagtcggcgt ggaaggcgtc ggggatgatc
     2161 tccggatagc tgagtcgatc cggcagcagc gggaaggtgt gggcgtagat cgcttcgaga
     2221 acgccgatac caaagtattc gtggtcggcc gtggagaagg tggccgcggc ctgccagagc
     2281 agatggcgat aggtgtcgtc gttcgcatag cgattgtgaa taactcgctc gcccagcgcc
     2341 tcgaccccgg ccagccaggc cgcgtccggc tgcccaaagc gttcgccaca cagagccacg
     2401 cgaaacggca gtccttcccc ggccagcgtc agcagcacgt ccatgaagcg ctccggccgc
     2461 ttgtcgaatt ccaggcgctg gttccagaga atgagtggcg gcgcgccggc ggcggctgtc
     2521 tccggcggct ccaggcggcg caggtcgatg cccacaggca ggacggcgct ccggcggcgc
     2581 aggagaagga tggtatcgag ttcgttgtac tcggggtagt gacgcaggaa ggcgggcaag
     2641 gcggcgaaga acgtctccag gtggtagcgc gaattgaaga aaacgcggtc ggcggccagc
     2701 atggaggcgt aattgatgaa ggcgtagtgg cggtcgcgct cgcccaactg gcgacgcatt
     2761 gggccggtgg ccgggtcgtc gggcagaggg taagtcagct ggttctcgtg catgtagagc
     2821 gccgcgggga tgccggccgt gtggcggcgc gttaaggcca gaaaggtggt caggtcaaac
     2881 aggtcagtag cgagaatgag gtcgggcagc ggctggctgg cggcctggct cagccattgc
     2941 cgcgccagcg taaccgcccc gccatgcatg cgccacttcc agaagtgggc cggcagggtt
     3001 agcagcgcaa ttgcgtgggc gctggcgcag gcgtagccct cggcccaggc gcggtggctg
     3061 ccgccgtgat agggcgacag cagcaggatg cggccggtca tctacgttta cccgtcatca
     3121 cggcaaaatc gcgcttacaa ttcgaccact tccacatgtt cgtctatact tcctgtcgat
     3181 tacagtcagg aatagag
//
LOCUS       DAOSMQ010000378         3192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30277_length_3192_cov_4.607906, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000378 DAOSMQ010000000
VERSION     DAOSMQ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30277_length_3192_cov_4.607906"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK829_11495"
     CDS             <1..440
                     /locus_tag="PK829_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41880.1"
                     /translation="FGWMAAPLPGWMVAGLAVLTGLAVVGLGLKMASSQWPVISDQRP
                     AAAGRRPPVNRQHILVLGLTVLLTAALHVYYNLTFVQHQGRYLYPALIPIAVGFVAGW
                     AYWLRPAARRWPWAAWLLPLGVAAVLGGIALFALWRILPGLAP"
     gene            complement(458..1261)
                     /locus_tag="PK829_11500"
     CDS             complement(458..1261)
                     /locus_tag="PK829_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HOU41881.1"
                     /translation="MPSPFPGMDPYLEGEMWQEFHETLAGAIRAQLLAVLPPHYVALL
                     AKRYVLNNQSVGLLSLPEEQRVIYPDVHVVHPSSRIAETAVAYETQALASPATQLISP
                     MAEEVPVMSVEVRDIANRRLVTVIEILSPVNKMGEGAVEYARKRQDVLKTATHLLEMD
                     LLRRGLRIGLAGELPPADYYVYLSRFTHRPYTDVWPVALRDPLPVVPVPLLPPDADVP
                     LNLQRAVNACYELVHYERLLDYAAAPPPPPLSEADAAWMGTVVSRPVKQ"
     gene            complement(1304..1537)
                     /locus_tag="PK829_11505"
     CDS             complement(1304..1537)
                     /locus_tag="PK829_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41882.1"
                     /translation="MTTPIEVDCTFEADGRVRVRRVRLGRPWQPVEQGRQWADADGRH
                     VLIMLAGGVRELLLRADTLTWELHELPGTRRPA"
     assembly_gap    1589..1688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1693..2460)
                     /locus_tag="PK829_11510"
     CDS             complement(1693..2460)
                     /locus_tag="PK829_11510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU41883.1"
                     /translation="MPELTAIILTKNEARHVAECVASLRWADRVLVFDSLSDDDTVAL
                     ARAAGAEVAARPFDNYAAQRNAALDSVTTDWVFFVDADERGTPALAAEVRQVIATRPE
                     DGWYVPRHNYIFGRLTLGAGWYPDYQFRLLRHGRARYVRPVHELGEVDGAIGYLQNPL
                     IHYNYRDRAQFHAKQRFYVETDAGILRDAGVRPKPRNYVLQPLRQFYWRYVTLGGWRD
                     GLHGLRLSLYMGYYEWRKYRRLRELWRSPFPLPPGEG"
     gene            complement(2572..>3192)
                     /locus_tag="PK829_11515"
     CDS             complement(2572..>3192)
                     /locus_tag="PK829_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOU41884.1"
                     /translation="DVRAETLQPREAHAWFVSGHSGDHILIRVTPLSPGLDLDVWLLD
                     GAIERVAAVDAFAAGEPETIELTLTADGQYIVFVRDFNGEPGDYEIALGAAPVATPER
                     GGTLNIGDTIIGVIRPGTAVAWSFQVAAGDAINLTVTPAEAGSDIVLQLQGPDGLTAL
                     EVDAGSAGEAETVSGFVVPTAGPWRIVLREYFAQTANFRLSLTRAR"
BASE COUNT          505 a         1016 c         1059 g          512 t
ORIGIN      
        1 agttcggctg gatggccgcg ccgctgccgg ggtggatggt ggccgggctg gcggtgctga
       61 cggggctggc ggtggtgggt ctggggctaa aaatggccag tagtcagtgg ccagtgatca
      121 gtgatcagcg gccggcagcc gctggccgcc ggccgccggt aaacaggcag cacatactcg
      181 tgctgggcct gaccgtactg ctgacggcgg cgctgcacgt ttactacaac ctgaccttcg
      241 tacagcacca gggccgctat ctctacccgg cgctcatccc gattgcggtg ggctttgtgg
      301 ccgggtgggc gtactggctg cggccggcgg cacggcgctg gccgtgggcg gcgtggctgc
      361 tgccgctggg cgtcgccgcc gtgctggggg gcattgccct atttgccctg tggcggattt
      421 tgccggggct ggcgccgtga gctactgatt cactgaccta ctgcttcact ggcctactga
      481 ccacagtacc catccaagcc gcgtccgcct cgctcaacgg cggcggcggc ggcgcggcgg
      541 cgtagtcgag cagccgctcg taatgcacca gctcatagca ggcgttgacg gcgcgttgca
      601 ggttgagcgg cacgtcggca tcgggcggca ggagcggcac aggcacgacc ggcagcgggt
      661 cgcgcagggc caccggccag acatcggtgt agggacggtg ggtgaagcgg ctgaggtaca
      721 cgtagtagtc ggccggcggc aactcgcccg ccaacccaat ccgcaggccg cgccgcagca
      781 ggtccatctc cagcaggtgc gtcgccgtct tcagcacgtc ctgtcgcttc ctagcgtact
      841 ccaccgcgcc ctcgcccatt ttgttgaccg gcgaaagaat ctcaatgacc gtcaccaagc
      901 gccggttggc aatgtcgcgc acttcgacgc tcatcaccgg cacttcctcg gccatcgggc
      961 tgatcagttg ggtagcgggc gacgccaacg cctgtgtctc gtaggccacg gcggtttcgg
     1021 caatgcgtga cgatggatga accacgtgta cgtcgggata aatgacccgc tgctcctcag
     1081 gcaagctgag caggccaacg gattggttgt tcagcacgta gcgtttggcc aatagggcca
     1141 cgtaatgcgg cggcaagacg gccagcagtt gcgcgcgaat agcgccggcc agcgtctcgt
     1201 ggaactcttg ccacatctcg ccctcaagat atggatccat gccgggaaat ggagatggca
     1261 taagtcggcc tccactcctt attatagcac gtcgctcgtc cggctacgcg gggcgacggg
     1321 ttccaggcaa ctcgtgcagc tcccacgtca gcgtatcagc gcgcagaagt agctcgcgca
     1381 cgccaccggc gagcatgatg agcacgtggc ggccgtcggc gtcggcccac tggcggccct
     1441 gctccaccgg ctgccacggc cgtcccagcc gcacgcgccg gacgcgcacg cggccgtcgg
     1501 cctcgaaggt gcaatcgact tcaatgggtg tggtcatgat cggtgtatac cctcttccac
     1561 agggagaggc aacgccaatc ccccgcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnct ccctaaccct ctcccggggg gagagggaac ggggaacgcc agagttcgcg
     1741 gaggcggcgg tacttgcgcc actcgtagta gcccatgtag agactcagcc gcagcccatg
     1801 tagcccgtcg cgccagccgc ccaaagtgac gtagcgccag tagaactgcc gcagcggttg
     1861 caggacgtag ttgcgcggct tcggccggac gcccgcgtcg cgcaggatgc cggcgtccgt
     1921 ctccacgtag aagcgctgct tggcgtggaa ttgcgcccga tcgcggtagt tgtagtggat
     1981 gagcggattt tgcaggtagc caatggcccc atcgacctcg cccagctcat gcaccgggcg
     2041 gacgtagcgg gcgcggccgt ggcgcaacag gcggaactgg taatcgggat accagcccgc
     2101 gcccaacgtc agccggccga agatgtagtt atggcggggc acataccagc cgtcttccgg
     2161 ccgcgtggcg atgacctggc gcacctcggc cgccagcgcc ggcgtgccgc gctcgtcggc
     2221 atcgacgaaa aacacccagt cggtcgtcac gctgtcaagg gcggcgttgc gctgggcggc
     2281 gtagttgtcg aacggccgcg cggctacctc ggccccggca gcgcgggcca gggccaccgt
     2341 gtcgtcgtcg ctgagcgagt cgaacaccag cacgcggtcg gcccagcgca ggctggcgac
     2401 gcactcggcc acgtggcggg cctcgttttt ggtcaggatg atggcagtca attccggcat
     2461 tcggatcaaa gttttaacgc aaggacgcaa agaagcaagg acgccaagaa aacgtcatcg
     2521 ctctattctt ggcgtcttag cgtcctggcg tccttgcgtt aacctcttct ctcatctcgc
     2581 ccgcgtcagc gagaggcgga agttggccgt ctgggcgaag tattcgcgca ggacgatgcg
     2641 ccacgggccg gccgtgggca cgacgaagcc ggacacggtc tcggcttcac ccgccgaccc
     2701 ggcgtcgacc tccagcgccg ttagcccgtc cggcccttgc aattgcagaa caatgtcgct
     2761 gccagcctca gccggggtga cagtaaggtt gatggcgtcg ccggcggcga cctggaacga
     2821 ccaggccaca gccgtgcccg gccggatgac gccgatgatg gtgtcgccga tattcagcgt
     2881 gccgccgcgc tccggcgtag ccacgggggc cgcgcccagg gcgatctcgt agtcgcccgg
     2941 ctcgccgttg aagtcgcgca cgaagacgat gtactgcccg tcggcggtca gcgtcagctc
     3001 gatcgtctcc ggctcgcccg cggcgaaggc gtccacggcg gcgacgcgct cgatggcgcc
     3061 gtccagcagc cagacgtcca ggtcgaggcc ggggctgagc ggcgtcacgc ggatgaggat
     3121 gtggtcgccg ctgtggcccg acacaaacca ggcgtgggcc tcgcgcggct ggagcgtctc
     3181 agcgcgcacg tc
//
LOCUS       DAOSMQ010000379         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30484_length_3175_cov_3.693910, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000379 DAOSMQ010000000
VERSION     DAOSMQ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30484_length_3175_cov_3.693910"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1095
                     /locus_tag="PK829_11520"
     CDS             148..1095
                     /locus_tag="PK829_11520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU41885.1"
                     /translation="MISWRPGPLRETLLSFVDAVTDPGDPFYVPPAGRLAVFDHDGTL
                     WCEKPLMVHIYALLARYRQLARREPLGLSRRALRAVFANDHSDFDNVFQRSEWAEILG
                     DLAGVPFSAMSDVDFDAWVRHWAGRWRHPRFGVGVRGLVYRPMVELLHLLQAREFTVA
                     ITTADEAAFVRVLSAELYGVAPGRVLGSNFVPQGSRLDPSLLLRGYHPDFFHDGEDKP
                     RCIERELGQRPLLAAGNSDGDLALLRWTAEGQGARLPLVVRHTDAAREYAYDRRAGRL
                     LAEAAARGWPVVDMARDWGTVLGEGEGTGSRGQGLGEAR"
     gene            1418..2836
                     /locus_tag="PK829_11525"
     CDS             1418..2836
                     /locus_tag="PK829_11525"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOU41886.1"
                     /translation="MNRRLVLMALALLVSLMLPLGLAVAQGDAPAASGVSGRLLYNGL
                     VGNDANTQEVFTMNADGTGQTRLTFNDGYDGEAAWSPDGTRIAFVAEVPMTYPYQLYV
                     MNADGSNPVHLVDPADHPGRPTWSPDGTKLAFMVNGSEQLYIVNSDGTGLVAIQGVSG
                     TGPSWSPDGQLIAFAGYGVNDRFDIFVVRPDGTGLTNLTKTKSHDWSPAWSPDGGRIV
                     FYRDFGKGNDELYTMNRSGGDRIRLTNTPGSERWPSWSPDGQQIAYVSWRPNEEDELV
                     AHIFTMNADGSNRKQITNGLGEYYPSWQPAASGPATTFTPTNDATVMQARPRNVYGAK
                     PVLQVKDAAKDINSYVKFNVTGLTGAAQSATLRLWVTNASPDGGAVYAVSPTYLNTTT
                     QWLETGLNWNNAPAITGAPLGSLGPVTAGRWVEIDVTAGVVAALSSGSRVSFALTNDS
                     TNLAAYSSLNGVHPPELVVVTD"
     gene            complement(2933..>3175)
                     /locus_tag="PK829_11530"
     CDS             complement(2933..>3175)
                     /locus_tag="PK829_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41887.1"
                     /translation="ATAVGDAADTAAQKMTDAASAVAGKAHDAADAAGSRAQDAAAAL
                     EDALPERQCAAITKAGTRCKRPAMDGSDFCAIHQVA"
BASE COUNT          500 a         1086 c         1056 g          533 t
ORIGIN      
        1 gccgtagcac aggctgtcag cctgtcgccg ggcagcctga caggctgcgc tacagagcag
       61 atcgtcccgc ccgccgtagc acaggctgtc agcctgtcgc cgggcagcct gacaggctgc
      121 gctacagggc atataatcct gtctgttatg atctcctggc gtcccggccc ccttcgcgag
      181 acgctgctgt cgttcgttga tgctgtcacc gatcccggcg atccgttcta cgtgcccccg
      241 gccggacggc tggccgtctt tgaccacgac ggcacgctgt ggtgcgagaa gccgctgatg
      301 gtccacatct acgccctgct ggcccgctac cgccagttgg cccgccgcga gccgctgggc
      361 ctctcgcgcc gggcgctgcg ggccgtcttc gccaacgacc atagcgactt cgacaacgtc
      421 ttccagcgca gcgagtgggc cgagattctg ggcgacctgg ccggcgtgcc cttcagcgcc
      481 atgagcgacg tcgacttcga cgcctgggtg cgccactggg ccggccgctg gcggcatccg
      541 cgctttggtg tcggcgtgcg cgggctggtc taccggccga tggtcgagtt gctccacctg
      601 ctgcaagcgc gcgagttcac cgtggccatc acgacggccg acgaggccgc tttcgtccgt
      661 gtcctctcgg ccgagctgta cggcgtggcc cccggccgcg tcctgggcag caacttcgtg
      721 ccccagggca gccgccttga cccgtcgctg ctgttgcgtg gctatcaccc cgacttcttc
      781 cacgacggcg aggacaagcc gcgctgcatc gagcgggagc tgggccagcg gccgctgctg
      841 gccgcgggca acagcgatgg cgacctggcc ctgttgcgct ggacggccga ggggcagggg
      901 gcgcgcctgc cgctggtcgt ccgccacacc gacgccgccc gtgaatatgc ctacgaccgc
      961 cgcgccggcc gcctgctggc cgaggccgcc gcccgcggct ggccggtggt ggatatggcg
     1021 cgggattggg gcacggtgtt gggggagggg gaggggacag gttccagggg ccaggggcta
     1081 ggggaggcac gctaaccggc tgctctactg acctgctgcc tcactgtccc cctgtcctac
     1141 tgtccgccag ttagcaagcc cactgtcatc cgatttacag cgctctctgg gcaattgggt
     1201 atactgtaac caggtaaaaa gccagcggct ttttccgcaa agcaaagtat tggggccggg
     1261 ttgtattgtc aagccacaga cccgtgggtg gcgccagcca cccacctgcc tatccgtccg
     1321 tcatccggtt ccgtcaagcc gcggtcggtg cggcccaacc cgccgtcacg acctgagtag
     1381 cctggtcgtt tggccggtct acaggagcca caaacccatg aaccgcagac tcgttttaat
     1441 ggctctggcc ctgctggtga gcctcatgct gccgttgggc ctggccgtcg cccagggcga
     1501 cgcgccagcc gcttcgggtg tatccgggcg gcttctctac aatggtctgg tgggcaacga
     1561 cgctaacaca caggaagtct tcaccatgaa cgcggatggc acgggccaga cgcgcctgac
     1621 cttcaacgac gggtatgacg gcgaagcggc ctggtcgccc gatggcacgc gtattgcctt
     1681 cgttgccgaa gtgcccatga cctaccccta tcaactctac gtgatgaacg ccgacggtag
     1741 caacccggtt caccttgtgg atccggctga tcatcccggc cgcccaacct ggtcgcccga
     1801 cggcacgaaa ctggccttta tggtcaatgg cagcgaacag ctctacatcg tcaacagcga
     1861 tggcacgggc ctggttgcga ttcagggcgt gtcgggcacc gggccgagct ggtcgcccga
     1921 cggccagctg atcgccttcg ccggctatgg cgtcaacgat cgcttcgata tctttgtggt
     1981 caggcccgac ggcacggggc tgaccaacct gacgaagacg aagtcgcatg actggtcgcc
     2041 cgcctggtcg ccggacgggg ggcgcatcgt gttctatcgc gatttcggca agggcaacga
     2101 tgagctatac accatgaacc gcagcggcgg cgaccgcatc cggctgacca acacgccggg
     2161 cagcgagcgg tggccgtcct ggtcgcccga cgggcagcag atcgcctacg tcagttggcg
     2221 gccgaatgaa gaggacgaac tggtcgccca catcttcacc atgaatgccg acggcagtaa
     2281 ccggaagcag atcaccaatg gcctgggcga gtattacccc agctggcagc cggcggcgag
     2341 cgggccggcg acgaccttca cccccaccaa cgacgctacc gtgatgcagg ccaggcccag
     2401 gaacgtctac ggcgcgaaac ccgtcctgca agtgaaggac gcggccaagg acattaatag
     2461 ttatgtgaag ttcaatgtca ccggcctgac cggcgcggcg cagagcgcca cgctgcggct
     2521 gtgggtcacc aacgccagcc cggacggcgg cgcggtctat gccgtctcgc ccacctacct
     2581 gaacacgacg acgcagtggc tggaaaccgg gctgaactgg aacaacgccc cggccatcac
     2641 cggcgcacct ctgggcagcc tcggccccgt gacggccggg cgctgggtgg aaatcgacgt
     2701 gacggccggc gtggtcgccg cgctcagcag cggcagccgc gtcagcttcg ccctgaccaa
     2761 cgactcgacc aacctggccg cctacagcag cctcaacggg gtgcatccgc cggaactggt
     2821 tgtcgtcacc gactgagtcg cgtcgccagc gtctgtactg ggaaataaag aggcgcgacg
     2881 ctccgttggg ggcgtcgcgc ctctgtccat ttccggacgt gcagccgcgc cgctaagcca
     2941 cctggtgaat ggcgcagaag tcgctgccgt ccatcgccgg gcgcttgcag cgcgtgccgg
     3001 ccttggtgat ggcagcgcac tggcgctccg gcagggcgtc ttccagggcc gcggcggcgt
     3061 cctgggcgcg gctgccggcg gcgtcggcgg cgtcgtgggc cttgccggcc acagcgctgg
     3121 cggcgtcggt catcttctgg gcggccgtgt cggcggcgtc acccacggcc gtggc
//
LOCUS       DAOSMQ010000380         3170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30553_length_3170_cov_4.252327, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000380 DAOSMQ010000000
VERSION     DAOSMQ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30553_length_3170_cov_4.252327"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PK829_11535"
     CDS             complement(<1..1118)
                     /locus_tag="PK829_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOU41888.1"
                     /translation="MRRLRQQLEEIESMSLAPYGLRSAASRGRHYPEPESATRTAFQR
                     DRDRIIHTTAFRRLMYKTQVFVFYEGDHYRTRLTHTIEVAQLGRSLARGLGGNEDLTE
                     AICLAHDLGHAPFGHAGEHSLNALMAEHGGFNHNFQSYRIVTELEERYPDFPGLNLTY
                     ETREGMLKHETDYDVSEAAEFEPEKRASLEAQIANLADEIAYDAHDLDDGLRAALFTL
                     EDLDELAIWRELCDSAGWAPGAHFSSLHRHEIIRELIGRSVTDVMAHTSAALEAANID
                     SPEKLQRSERNVVGYSPAFGEKVRALKSFLYARMYRHYRLVRMQTKAERFIAGLFEAY
                     MKEPDMLPRETWARLEHAPRARVVADYIAGMTDRYALD"
     gene            1338..2030
                     /locus_tag="PK829_11540"
     CDS             1338..2030
                     /locus_tag="PK829_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU41889.1"
                     /translation="MPPKRSGTVSRPIVIMIAIFATLLAAGLFLGIPYLQGRPAFPRG
                     TFPLLVEGNQIMVEMDPNGEVFLLGDAPLVDAGAGTGGPVVGQLVTSTPAIIATTAPP
                     TPVTPVIVPTAAPPTATPQPQASCVIFTSHTVQAGETLFSISRQYVTSIPLMARHGIS
                     STSLTPGAVISVPVGNPACCTNGWRPYVVESGDTWFGIAQQCGITVDSLLGGNGLSAG
                     ATLYMTSVICIP"
     gene            complement(2047..2202)
                     /locus_tag="PK829_11545"
     CDS             complement(2047..2202)
                     /locus_tag="PK829_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41890.1"
                     /translation="MTEETDEMTTYAPRGKWDFSDAQRIIIGLLLWLNIIVLVIAVLL
                     ILGVLTP"
     gene            complement(2261..3079)
                     /locus_tag="PK829_11550"
     CDS             complement(2261..3079)
                     /locus_tag="PK829_11550"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU41891.1"
                     /translation="MSDDDEELSEGAVAAALTTTWLGRAYRYRPAVDSTNDQMKRWAA
                     GGAPHGAVLLADYQSAGRGRLDRRWDAPPGTALLLSVLLRPAGWPAGRGAWLTMLAGL
                     AAAEAIEDVTGRPARLKWPNDVLLGEGETWRKAGGLLLDAALDANGGLEMAILGIGLN
                     VNIPAGALPPAATPATSLLVELGRPVARPALLAALLARLEGRYAAADAGQSPAEDWAA
                     RLVTLGRTVDVTAAGRAPLAGTAEGVDEWGRLLVRDATGQLHTVAAGDVTLRGR"
BASE COUNT          504 a         1108 c         1030 g          528 t
ORIGIN      
        1 tcatcgagcg cgtagcggtc ggtcatgccg gcgatatagt cggcgacgac gcgggcgcgc
       61 ggcgcgtgct ccaaccgcgc ccacgtctcc cgcggcaaca tgtccggctc cttcatatac
      121 gcctcgaaca agccagcgat gaagcgttcg gccttggtct gcatgcgcac cagccggtag
      181 tggcggtaca tacgcgcata gaggaagctc ttcagggcgc gcaccttctc gccaaatgcc
      241 ggcgaatagc cgacgacatt gcgctcagac cgttggagct tttccggcga gtcgatgttc
      301 gcggcctcca gcgcggcgct ggtgtgggcc atcacgtcgg tgacggagcg gccgatcagc
      361 tcacggatga tctcgtgccg gtgcagcgag gagaagtgcg cccccggcgc ccagccggcg
      421 ctgtcgcaca gctcgcgcca gatggccagt tcgtccaggt cttccagcgt gaacagggcg
      481 gcccgcaggc cgtcatcgag gtcgtgggcg tcataggcga tttcgtcagc caggttggcg
      541 atctgggctt ccaatgaggc gcgcttttcc ggctcgaact cggccgcctc gctgacgtcg
      601 tagtcggtct cgtgcttcag catgccctcg cgcgtctcgt aggtcaggtt cagaccgggg
      661 aagtcgggat agcgctcctc caattcggtg acgatacgat agctctggaa gttatggttg
      721 aagccgccat gctcggccat cagcgcatta agggagtgtt cgccggcgtg gccaaaaggc
      781 gcgtggccca ggtcgtgggc caggcagatg gcctcggtca ggtcttcgtt gccacccagg
      841 ccgcgggcca gcgaccggcc gagctgggcg acttcgatgg tgtgggtcag acgagtgcga
      901 tagtgatcgc cctcgtagaa cacaaacacc tgcgtcttat acatgagccg gcgaaaagcc
      961 gtggtgtgga tgatgcggtc gcggtcgcgt tggaaggcgg tgcgcgtggc cgattctggc
     1021 tccggatagt gccggccgcg cgacgcggcg ctgcgcaacc catagggggc caggctcatc
     1081 gattcgatct cttctagctg ctgtcgtagg cgtcgcatgg gaaactgctc ctaagagggg
     1141 gccaatcgga tcgatcgcgc ccaggtagtt gcccagggcc tttccagagt cgtcgagcga
     1201 ccattgaccg gacaggtggc cgcccaccga tccggtcggc gcgcctggga tgactttgga
     1261 aaagcccagg tagttgccgc caagccatgc ggagtgtaac ataatcagtg acgcatcgca
     1321 ctttaggcga ggaccgtatg ccaccgaagc gttcaggcac tgtcagcagg cccattgtca
     1381 tcatgatcgc catcttcgcc acccttctgg cggcgggact attcctgggc atcccctacc
     1441 tgcagggccg gccggccttc ccgcgtggca cgtttccact gctggtcgaa ggcaatcaga
     1501 tcatggtgga aatggatccc aatggagagg tgtttctgct gggcgacgcg cctctagtgg
     1561 acgccggcgc gggcacgggc gggcctgtcg tcggccagct cgtcacctcc acaccggcca
     1621 tcatcgccac caccgcgccg ccgacgcccg tcacacccgt gattgtgcct actgccgcgc
     1681 cgccgacggc tacgccccag ccgcaagcca gttgcgtcat ctttacgagc cacaccgtcc
     1741 aggcgggcga gacgctattc agcatctccc gccaatacgt cacctccatc ccgctcatgg
     1801 cccggcacgg catctcctcc acgtcgctga cgcccggcgc ggtcatctcc gtgcccgtcg
     1861 gcaacccggc ctgttgcacc aacggctggc ggccctatgt ggtcgagtcg ggggacacgt
     1921 ggtttggcat cgcccagcaa tgcggcatca ctgtcgattc gctactgggt ggcaacggcc
     1981 tcagtgccgg ggcgacgctc tacatgacct cggtcatctg cattccctag ccgccaccgg
     2041 cgtgggctac ggggtcagca cgcccagtat aagcaggacg gcaatcacca ggacgatgat
     2101 gttcagccac agcaagaggc cgatgatgat ccgctgggcg tcgctgaagt cccacttgcc
     2161 acgcggcgcg taggtcgtca tttcgtctgt ttcctcggtc atggcagctc tctccaggcg
     2221 cgtgttggtt gatctagcgt agcgcaggat gagccaggcg tcaacgcccg cgcagggtca
     2281 catcgccggc ggcgaccgtg tggagttgtc cggtggcgtc gcgcaccagc agccgccccc
     2341 actcgtctac gccctcggcc gtgcccgcca gcggcgcgcg gccggcggcg gtcacgtcca
     2401 ccgtccgccc cagcgtcacc agtcgcgccg cccagtcttc ggccggcgac tgcccggcat
     2461 cggcggcagc atagcgcccc tccagccgcg ccagcagggc cgccagcagc gccggccgcg
     2521 ctaccggccg ccccaactcg acgagcaggc tggtggccgg cgtggccgcg ggcggcagcg
     2581 cgccggcggg aatgttgacg ttcaggccga tgccgaggat ggccatctcc agaccgccat
     2641 tggcgtccag ggccgcgtcg agcagcagcc cgccggcctt gcgccacgtc tcgccttcgc
     2701 ccaatagcac gtcgttgggc cacttgagcc gcgccggccg cccggtcacg tcttcgatgg
     2761 cctcggccgc ggccagcccg gccagcatcg tcagccacgc gccgcgcccg gccggccagc
     2821 cggcggggcg caacagcacc gacaacagca gcgccgtgcc cggcggcgcg tcccagcggc
     2881 ggtcgaggcg gccgcggccg gccgactggt aatccgccag cagcacggcc ccatgcggcg
     2941 cgccgccggc cgcccagcgc ttcatctggt cgttggtcga atccaccgcg gggcgatagc
     3001 gataggcgcg ccccagccag gtcgtcgtca gggccgctgc cacggccccc tcgctcaact
     3061 cctcatcgtc gtcgctcatg ccgccattct accagcttcg cggcggggtc gaagcgccga
     3121 ccgctgacca cagaccacag accacagacc acagaccatt gaccacagac
//
LOCUS       DAOSMQ010000381         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30794_length_3152_cov_5.020019, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000381 DAOSMQ010000000
VERSION     DAOSMQ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30794_length_3152_cov_5.020019"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK829_11555"
     CDS             <1..268
                     /locus_tag="PK829_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41892.1"
                     /translation="ASARRVLVWGPDEETALERRQLAVQILGERGADVHYQHLAGGDA
                     QRVLTWVNKQGGSLLILPTGEGAWPAGTQAALLDEAQPHLLLVR"
     gene            340..2217
                     /gene="uvrC"
                     /locus_tag="PK829_11560"
     CDS             340..2217
                     /gene="uvrC"
                     /locus_tag="PK829_11560"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU41893.1"
                     /translation="MSYTPSDHLAEVLNSLPTKPGVYLHKDAEGNVLYVGKAINLRNR
                     VRSYFHSAVDSVKTLRLRQHIADIEVIITDSELEALLLEMNLIKQYQPRFNVRLKDDK
                     RYPYIKVHWADPFPKVTVTRRMVRDGGRYFGPYTSAWAVQQTLDLLRKVFPYLTCDRV
                     ITGQDERACLYYDIRLCNAPCIGVVNQEQYRATIKQLMDFLDGKSDHIVRGLEQRMAT
                     AAENLDFERAAEIRDQLKAIERITARQKIIGSDETDQDVIAFARDDGEACVQVFFIRH
                     GKLIGREYFMLDNAEGESDQDVLQEFLTAFYEDAAYVPKEVLLPAEIEEARIIEEWLR
                     RKRDTKVTLQVPRRGKKRELVEMAAENAHDTLAALRQQWAADRSKHVTAITELQEALK
                     LPAPPARIECYDISHTQGQQTVGSMVVFVQGAPQKSDYRRFNVQSATNDDYSSMREVL
                     TRRFQRYRDTLAGELHDPGQIKPRAERPTAWAILPDLLIIDGGKGQLAVGQEVLRLFD
                     LEGEVPLAALAKQEEELFQPDRPQSVLLPRRSEALYLVQRVRDEAHRFANEGHRKRRS
                     KVGVASILDSVPGVGPRRRQLLLKRFGSLEALREASVEEISAVPGIPVDVAASIKSYL
                     Q"
     gene            complement(2229..2816)
                     /locus_tag="PK829_11565"
     CDS             complement(2229..2816)
                     /locus_tag="PK829_11565"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU41894.1"
                     /translation="MTTMPPPRPFQILASRIAWECPWYRVRQDDILTPDGRPGVYNVI
                     EKADAVWIVPVTAAGQVLMVYQYRYTVDEWCWEVPAGSVKPGQTREEAAREELREEVG
                     GEGGELTYIGRSFLANGICNEVGHFFLATGVEVGPTHHEAAEVMHVYHLPVAEALRMA
                     RAGEISDGPTALALLLCAERLLALAGAARDDMAAE"
     gene            complement(2823..>3152)
                     /locus_tag="PK829_11570"
     CDS             complement(2823..>3152)
                     /locus_tag="PK829_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41895.1"
                     /translation="RPRSAAEAEADSTRYLAPDTLPGPRLLLDTPHGRLYGGTGATGD
                     WALAAALATAVPRLMLAGGLTPDNVAAAVACVRPLAVDVAGGVESAPGRKAPDRVRAF
                     IAAAKAA"
BASE COUNT          554 a         1050 c         1054 g          494 t
ORIGIN      
        1 ggccagcgcg cggcgcgttc tggtctgggg gccagacgag gagactgccc tggagcgccg
       61 ccaactggcc gtgcagatcc tgggcgagcg cggggccgac gtgcactacc agcacctggc
      121 cggcggcgat gcgcaacgcg tgctgacctg ggtcaataag cagggcggca gcctgctcat
      181 cctgcccaca ggcgaaggcg catggccggc gggcacgcag gcggctctgc tggacgaggc
      241 gcagccacat ctattattag tcaggtaaaa tgcgaatagg tgtgctaaaa tatggatccg
      301 cggacgcgcc gccggctggc gcgtcgcgcc tgagcgccga tgagttatac gcccagcgat
      361 cacctggccg aagtcctgaa tagcttgccg accaagcctg gcgtctatct gcacaaggac
      421 gccgagggca atgtcctcta cgttggcaag gcgatcaacc tgcgcaatcg ggtgcgctcc
      481 tatttccaca gcgcggtcga ttcggttaag acgctgcgcc tgcgccagca catcgccgac
      541 atcgaggtca tcatcaccga ctccgagctg gaagcgttgc tgctggagat gaacctcatc
      601 aagcagtacc agccgcgctt caatgtgcgc ctgaaggacg acaagcgcta cccttacatc
      661 aaggtgcact gggccgaccc gttccccaag gtgaccgtaa cgcgacgaat ggtgcgcgac
      721 ggcggccgtt actttggccc ctacacttcc gcctgggccg tgcaacagac gcttgatttg
      781 ctgcgcaagg tctttcccta tctaacctgc gaccgcgtca tcactggcca ggacgagcgc
      841 gcctgcctct actacgacat caggctgtgc aacgcgccct gcattggcgt ggtcaaccag
      901 gagcagtacc gggcgacgat caagcagctc atggacttcc tggatggcaa atccgaccac
      961 atcgtccgcg ggctggagca gcgcatggcc acggcggccg agaacctcga cttcgagcgc
     1021 gccgccgaaa tccgcgacca actcaaggcc atcgagcgca tcaccgctcg ccagaagatc
     1081 atcggctccg acgagaccga tcaggacgtg atcgcctttg cccgcgacga cggcgaagcc
     1141 tgcgtgcagg tgtttttcat tcgccacggc aagctcattg gccgcgagta tttcatgctc
     1201 gacaacgccg agggcgagag cgatcaggac gtattgcagg agttcctgac ggcgttctat
     1261 gaggacgctg cctacgtgcc caaagaggtc ttgctgccgg ccgagatcga agaggcgcgc
     1321 atcattgagg aatggctgcg ccgcaagcgg gacaccaaag tgaccttgca ggtgccgcgg
     1381 cggggcaaga agcgcgaatt ggtggagatg gccgccgaga acgcccacga tacgctggcg
     1441 gcattgcggc agcagtgggc cgccgaccgc tccaagcacg tcaccgccat caccgaactc
     1501 caggaggccc tgaagctgcc cgcgccgccg gcacgcatcg agtgctacga catcagccac
     1561 acccagggcc agcagacggt cggctcgatg gtcgtcttcg tgcagggcgc gccgcagaag
     1621 agcgactacc ggcgcttcaa tgtgcagtca gctaccaacg acgactatag ctccatgcgc
     1681 gaggtgctga cgcgccgctt tcagcgctat cgcgacaccc tggccggcga actgcacgac
     1741 cccggccaga tcaagccgcg cgcggagcgg ccgacggcct gggccatcct gcccgaccta
     1801 ttgattatcg acggcggcaa ggggcaattg gcggttggtc aggaagtgct gcgtctgttc
     1861 gacctggagg gcgaagtgcc gctggcggcg ctggccaagc aggaggaaga gcttttccag
     1921 cccgaccggc cgcagtcggt gttgctgccg cggcggtcgg aggcccttta cctggtgcag
     1981 cgcgtccgcg acgaggccca ccgcttcgcc aatgagggcc accgcaagcg ccggtcaaag
     2041 gtcggcgtgg cctccatcct cgacagcgta cccggcgttg gcccgcggcg gcggcaactg
     2101 ctgctgaagc gcttcggctc gctcgaggcg ttgcgcgagg cgtcggtcga ggagatcagc
     2161 gctgtgccgg gcatccccgt cgacgtggcc gcgtcgatca agtcctactt gcagtagccc
     2221 cgcggccgct attcggccgc catatcgtcc ctggctgctc cggccaaggc caacagccgc
     2281 tccgcgcaga gcagcagggc cagcgccgtt ggcccgtcac tgatctctcc ggcgcgggcc
     2341 atgcgcaacg cctcagctac gggtaggtgg tagacgtgca tcacttcggc cgcctcgtgg
     2401 tgggttggcc cgacctcgac ccccgtggcc agaaagaagt ggccgacctc gttgcagatg
     2461 ccattggcca ggaaagagcg gccaatgtag gtcagctcgc ccccttcgcc accgacttcc
     2521 tcgcgcaact cctcgcgggc cgcttcttcc cgcgtctgcc ctggcttgac gctgccggcg
     2581 ggcacttccc aacaccactc atcgaccgtg tagcggtact ggtagaccat gagcacctgg
     2641 ccggcggcgg tcacgggcac aatccagacg gcatcagcct tctcgatgac gttgtagacg
     2701 cctggccggc cgtcgggcgt gaggatgtcg tcttgccgca cgcgatacca ggggcactcc
     2761 caggcgatgc ggctggcaag gatttggaag gggcgaggcg gtggcatggt ggtcatagac
     2821 gctcaggcgg ctttggcagc ggcgatgaag gcgcgcacgc ggtcgggggc cttgcggccg
     2881 ggggcgctct cgacgccgcc ggcgacatcg acggccaacg gtcgcacgca ggccacggcg
     2941 gcggcgacgt tgtcgggcgt caggccgccg gccagcatca ggcggggcac agccgtggcc
     3001 agcgccgccg ccagcgccca gtcgcccgtt gcgcccgtgc cgccgtagag ccgcccgtgg
     3061 ggcgtgtcca acagcaggcg cgggccgggc agcgtgtcgg gcgcgagata gcgggtgctg
     3121 tcggcctcgg cctcagccgc cgagcgggga cg
//
LOCUS       DAOSMQ010000382         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30818_length_3150_cov_4.215832, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000382 DAOSMQ010000000
VERSION     DAOSMQ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30818_length_3150_cov_4.215832"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PK829_11575"
     CDS             complement(<1..625)
                     /locus_tag="PK829_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOU41896.1"
                     /translation="MTYPRFRKVLIANRGEIALRLIRACHELGIRAVAVYSDVDAGAP
                     WVRAADEAHPLHGSAAAETYLDRGKLLTIAANANADAIHPGYGFLSENAAFAGAGVGE
                     GGVFVGPTPAAMRALGSKAAARSLAEAHGVPVVPGVDGEGQSDDELAAAAAAIGYPVL
                     IKASAGGGGKGMRVVGAPDELRDALQAARQEAQSAFGDPHVLLEKYFT"
     gene            1358..2152
                     /locus_tag="PK829_11580"
     CDS             1358..2152
                     /locus_tag="PK829_11580"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOU41897.1"
                     /translation="MFPPTTAAAVKTESGVIGIVGGVGPLAGLDLQAKIIRQTIAGRD
                     QEHLPVLSLSWPGPIADRTEYLLGRVAANPAHALLAQLRLLAAAGATVAAIPCNTAHA
                     PAIFDIVRAGVAGLPLRLLHMIAETAAHLAAEHPELRTVGVLSTTGTWRARLYPQLLE
                     PRGLRVVVPDATLQETIHAAVYDPAHGIKPAGRATAQARAGLEQGIAALRAAGAEAII
                     LGCTEMPLAFPERRYADLPLIDSTLILARALIRAVAPERLRPWEEA"
     gene            2149..>3150
                     /locus_tag="PK829_11585"
     CDS             2149..>3150
                     /locus_tag="PK829_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41898.1"
                     /translation="MNIWKQWKLAALVALLALAACNGAATTGETTTTSVPAATATPAT
                     PAATTAVVLNTPEAPPVARDTLAGPIIGLNLENTYEEYYLALFDVGTNTFRELPATIG
                     LVMPYAARWFDGGCLIYADGHLLDLHGEFVWSVPDEVLAQTVDWHNSFLSPDRAWLAT
                     VVDDAGARHVEVIRLSAPFDRVRLTEGGGGHPGAVLWAAAADGLWLFFSDNSGGVLQV
                     FRAHPDGTGREQLTAHAASPALIVALALSPDGRRLAYGARNLAVPSSPYTYRVEDEGW
                     VGIVDLATGEARRVALPGLAGVEFGQGLVWSGDSTRLLAVGDSLPLATGDPLAGRQVH
                     "
BASE COUNT          503 a         1047 c         1086 g          514 t
ORIGIN      
        1 cggtgaagta cttctccagc agcacgtggg ggtcgccgaa ggcggactgg gcctcctgcc
       61 gggcagcctg cagggcgtcg cgcagttcgt ccggcgcgcc gacgacgcgc atgcccttgc
      121 cgccgccgcc ggcgctggcc ttgatgagca ccgggtagcc gatggccgcg gccgccgcgg
      181 ctaactcgtc gtcgctctgg ccctccccat cgacgccggg gacgacgggc acgccgtggg
      241 cttcggccag ggaacgggcg gcggccttgc tgcccagggc gcgcatggcc gccggcgtcg
      301 ggccgacgaa aacgccgcct tcgccgacgc ctgcgcccgc gaaggcggcg ttttcgctca
      361 ggaagccgta gccggggtgg atggcgtcgg cgttggcgtt ggcggcgatg gtcagcagtt
      421 tgccgcggtc gaggtaggtc tcggcggcgg cactgccgtg gagcgggtgg gcctcgtcgg
      481 cggcgcgcac ccacggcgcg cccgcgtcca cgtcggagta gacggccacg gcgcggatgc
      541 ccaactcgtg gcaggcgcgg atgaggcgca gggcgatctc gccgcggttg gcgatgagga
      601 ctttgcgaaa gcgagggtag gtcatgaggg aagtataccg tgatatattt gttttaactg
      661 tgatgacgga ttaaacgatg tttcgcactg cctacgggaa caactccgcc tgtagatatt
      721 tgctatacaa ctggcactat gcacgcattc ttccattatt gattgtagcg acaatgcttg
      781 ccggctgtcg cccaacgcaa cagagcaggg agccactacc gacgcaagat agttcaggct
      841 tgtcggaaat actacgagcg ttcccgcaag aacggccgcc agttgacggc gagcggataa
      901 gatatacctc cgccgtctgg ggcacgctcg tattagaaaa tggctgcctc cagctgatta
      961 cggctgagga tttcaccgac agggttgaaa gctatctcat catttggccg gacgattact
     1021 ggcctgaaat tgaagacagt agtggagacg tagttgttta tgatggttcc ggggaaatcg
     1081 cggcgcgcgt tggacagcct ctttatatgg gcggggccga gatacctgca acaaagccta
     1141 tttcggagat acccttagcg ttatcgagcg gcctgcagga gccaacgccc cccgaatgcc
     1201 cagggcctta ttggcttgcg ggaagcattg agatgcagaa tgggccaccg gccgattagg
     1261 ctctcttgaa agaaggacga actctgtcat cgatttgaaa cagcggcctc ctcgtgatac
     1321 gtttcaccca agcgagacgt atcatggcag gagagctatg tttccaccaa ccaccgccgc
     1381 cgcggtgaaa accgagtcgg gagtcatcgg catcgtcggc ggtgtcgggc cgctggcggg
     1441 gctggacttg caggccaaga tcatccgcca gaccatcgcc gggcgcgacc aggagcatct
     1501 gcccgtgctc agcctctcct ggccggggcc gattgctgac cggacagaat acctgctcgg
     1561 ccgggtcgcg gcgaacccgg cccacgccct gctggcgcaa ctgcggctgc tggccgcggc
     1621 cggcgcgacc gtcgccgcca tcccctgcaa caccgcccac gccccggcca tcttcgacat
     1681 cgttcgcgcc ggcgtggccg ggttgccgct gcggctgctg cacatgatcg cggaaacagc
     1741 cgcccacctg gccgccgagc accccgaact gcggacggtc ggtgtcctct cgacgacggg
     1801 cacgtggcgc gcgcggctct atccgcagct actggagccg cgcgggctgc gcgtcgtcgt
     1861 ccccgacgcg accctgcaag agacgatcca cgccgccgtc tacgacccgg cccacggcat
     1921 caagccggcc gggcgggcca cggcgcaggc gcgggccggc ctggagcagg gcattgccgc
     1981 gctgcgggcg gccggggccg aggcgatcat tctcggctgc acggaaatgc cgctggcttt
     2041 tccggaacgg cgctacgccg acctaccgct gatcgactcg acgctgatcc tggcgcgggc
     2101 gctcatccgc gccgtcgcgc cagagcggct gcggccgtgg gaggaagcat gaacatctgg
     2161 aagcaatgga aactggccgc gcttgtggcc ctgctggcgc tggcggcctg caacggcgcg
     2221 gcgacgacgg gcgaaaccac gaccacgtcc gtacccgccg cgaccgcgac cccggccacg
     2281 cccgcagcaa cgacggccgt cgtcctgaat acgccggaag cgccaccggt tgccagagat
     2341 acgctggccg gccccatcat tggccttaat ctggagaaca cctacgagga atactatctg
     2401 gctctcttcg acgtgggcac gaacaccttc cgcgagttac ccgccaccat cgggctggtg
     2461 atgccctacg cggcgcgctg gttcgatggc ggctgcctga tctacgccga cggccacctg
     2521 ctggacttgc acggcgaatt tgtctggtcg gttccggacg aagtgctggc ccagaccgtg
     2581 gactggcaca actcgtttct ctcgcccgac cgcgcctggc tggccacggt cgtcgatgac
     2641 gccggcgcgc gccacgtcga ggtcattcgc ctcagcgcgc ccttcgaccg cgtgcgcctg
     2701 accgagggcg gcggcgggca tccgggggcg gtgctctggg cggcggccgc tgacgggctg
     2761 tggcttttct tcagcgacaa cagcggcggc gtcttacagg tcttccgcgc ccaccccgac
     2821 ggcacgggcc gcgagcaact gacggcccac gcggcgtcgc cggcgctcat cgtcgccctg
     2881 gcgctgtcgc ccgacggccg gcggctggcc tatggcgcgc gcaacctggc cgtgccgtcg
     2941 tcgccgtaca cctaccgcgt cgaggacgag ggctgggtcg gcatcgttga cctggcgacg
     3001 ggcgaggcgc gccgggtggc cttgccgggg ctggcgggcg tggagttcgg gcaggggctg
     3061 gtgtggagtg gcgacagcac gcggctgctg gccgtcggcg acagcctgcc gctggccacc
     3121 ggcgacccgc tggccgggcg acaggtgcac
//
LOCUS       DAOSMQ010000383         3143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_30914_length_3143_cov_4.476360, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000383 DAOSMQ010000000
VERSION     DAOSMQ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30914_length_3143_cov_4.476360"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PK829_11590"
     CDS             <1..1003
                     /locus_tag="PK829_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41899.1"
                     /translation="APGPGAPACPPEEAAARATAPAQASAGLPCALFPPDNVWNTPID
                     TLPRHALSDAYVDAIGRAAEFHADFGAGIWPPEDGGPIGIPYVVVPAGQPGVPVQFYY
                     DDQSDPGLYPIPPDPPIEGGPQSDGDRHILIVQAGTCRLYELYDAWPRDDGGWDAGSG
                     AIYDLGSHALRPSGWTSADAAGLPILPGLVRYDEVAAGEIAHALRFTAPETQRAFVWP
                     ARHFASDLTAPGYPPMGQRFRLRADFDMSGFAPEVQVILRAMQRYGIILADNGSPWYI
                     SGAPDERWNNDILRQLHQLTGDDFEAVDTSSLMIDPDSGRVAMRDVFAFLPTVAAPR"
     gene            complement(1086..>3143)
                     /locus_tag="PK829_11595"
     CDS             complement(1086..>3143)
                     /locus_tag="PK829_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU41900.1"
                     /translation="GADAATTILTYDPLVQAVAGRDGILEIRASDVTVRDLTVRDAPI
                     EGGVPLWAVRVWKDGGTLTNVSFDTVRFLNNAGRGLELHNNTTLNNLTITGSLFKDNP
                     THGIRLSSTTNVNGLTITHTTFENNGHSGLFQSVGGGYLTGLHIDNSTFTGNVVQAVL
                     LGNAHDGLIENSTFSGGGRGIAFINLADTANAIGDLEIRHNTLSDFAGTAIWVDVNNT
                     ALDDPLTIANNTITQDISLLTTSGATIAVGLAGAKTHAAVDLIANDVTLAGVFGTATA
                     AYGLKLAGGLTAVTISGNSLDGGNVGNNGGAPATSGLYLVSNSAGYGALNQSDSVAVS
                     TNTITGFVNGISIYDEVSAAFGGLPVVNGLSIARNSIAGNSAFGVRGGPSTAAEATCN
                     WWGAASGPSGQGPGTGDAVSTDVIFAPWLPNDDILNSLCGGVNDLFVGTNRGGSVGGV
                     NFNDVDILALDMQTGTWSKFFEGKDVNVSTNLTGFTFEPDGCLLVTFDGNEKKLGLGM
                     IIKPHDILKFCPTSLGETTTGTWSVYLDGSDVGLTTDGEKLDALELLPDGRILLSTKG
                     NFAVKNAANQTITGRDEDVLVFDPTTLGANTTGSFAVYLDGSDIPGMVKEDITGLYYN
                     PLNGDLHVTILGTFNVGGVAGDSNDIIILRPSGGGYTVLPYWNGTDDGWSYVLRSMHI
                     DLP"
BASE COUNT          502 a         1047 c         1025 g          569 t
ORIGIN      
        1 tgccccaggg ccaggggcgc ccgcttgccc gccagaggaa gccgcggcgc gggccactgc
       61 gcccgcccaa gccagcgccg gcctcccctg cgccctcttc ccacccgaca acgtttggaa
      121 cacgcccatt gacacgctgc cgcgccacgc gctgtccgac gcctacgtag acgccatcgg
      181 ccgggccgcc gagttccacg ccgacttcgg cgcgggcatc tggccgccgg aggacggcgg
      241 gccgatcggc attccctatg tcgtcgtgcc ggccggccag ccgggcgtgc ccgttcagtt
      301 ttactacgac gaccagagcg accccggcct ctaccccatt ccgcccgatc cgcctatcga
      361 gggcgggccg caaagcgacg gcgaccgcca tatcctcatc gtgcaggcgg gcacgtgccg
      421 gctttatgag ctatacgacg cctggccccg cgacgatggt ggctgggacg cgggttcggg
      481 ggccatctac gacctgggca gccacgccct gcggccgtcg ggctggacgt cggccgacgc
      541 cgccgggctg cccatcctgc ccggcctggt gcgctatgat gaagtcgccg ccggcgagat
      601 cgcccacgcc ctgcgcttca ccgcgccgga gacgcagcgc gccttcgtct ggccggcgcg
      661 ccacttcgcc tccgacctga ccgcgcccgg ctatccgccg atgggccagc gcttccgcct
      721 gcgggccgac ttcgacatga gcggcttcgc gccggaggtg caggtcatcc tgcgggccat
      781 gcaacgttac ggcatcatcc tggccgacaa cggctcgccc tggtacatct ccggcgcgcc
      841 cgacgaacgc tggaacaacg acatcttgcg ccagctccac cagctgaccg gcgacgactt
      901 cgaggccgtg gatacgtcgt ccctgatgat cgatcccgac tccggccgcg tcgccatgcg
      961 cgacgtgttc gctttcctgc ccaccgtggc cgcgccacgc tgagacccgc ccggccgcaa
     1021 cgaaaacggg ccgccggctg tccggcggcc cgtaacccat gaccgcggct ggtagtcgct
     1081 actccctaag gcagatcgat gtgcatgctg cgcagaacgt agctccagcc atcatctgtt
     1141 ccattccagt agggtagcac ggtgtagccg ccgccggagg gccgcaagat gatgatgtcg
     1201 ttggagtcgc cggccacgcc gccgacgttg aacgtgccca ggatggtcac gtgcaggtcg
     1261 ccgttgagcg ggttgtagta gaggccggta atgtcctctt tgaccatgcc cgggatgtca
     1321 gagccgtcca ggtacaccgc gaacgagcca gtggtgttgg ctcccagcgt cgtcgggtcg
     1381 aagacaagga cgtcctcatc ccggccggtg atggtctgat tggcggcgtt cttgaccgcg
     1441 aagttgcctt tggtgctgag cagaatccgg ccgtcaggca gcaattccag cgcgtcgagc
     1501 ttctcaccgt cggttgtcag gccgacgtcg gagccgtcga ggtaaacgct ccacgtgccc
     1561 gtcgtcgtct cgcccagcga ggtcgggcag aacttcagga tgtcatgcgg cttgatgatc
     1621 atccccagac ccagcttttt ctcgttgccg tcgaaggtga ccagcaggca gccatctggc
     1681 tcaaaggtaa agccggtcag attggtgctg acgttgacat ccttgccctc aaagaatttc
     1741 gaccaggtac ctgtttgcat gtccagggcc aggatatcga cgtcgttgaa gttgacaccg
     1801 ccgacgctgc cgccccgatt ggtgcccaca aataggtcat tgacgccgcc acagaggcta
     1861 ttcagaatat cgtcgttggg tagccagggc gcgaagatga cgtcagtgct aacggcgtcg
     1921 cccgtgcccg gcccctgccc gcttggcccg ctggccgcgc cccaccagtt gcaggtggcc
     1981 tcggcggccg tgctcggccc gccgcggacg ccaaaagcgc tgttgccggc aatgctattg
     2041 cgcgcgatgc tcagcccgtt gacgaccggc agcccgccaa aggccgcgct aacctcgtca
     2101 tagatactga tgccgttgac gaaaccggtg atggtgttgg tcgagaccgc gacgctatcg
     2161 ctctggttga gcgcgccgta gccggccgag tttgaaacga gatagaggcc gcttgtggct
     2221 ggcgcgccgc cattgttgcc aacgttaccg ccgtcaaggc tattgccgct gatggtcaca
     2281 gcagttaggc cgccggccag cttgaggcca taggcggccg tggccgtccc gaacacgccc
     2341 gccagagtca cgtcgttagc gatcagatcg acggcggcgt gggtctttgc cccggccagg
     2401 ccgaccgcaa tggtcgcccc gctcgtggtc agcaggctaa tgtcctgcgt gatggtgttg
     2461 ttggcaatcg tcagcgggtc atcgagggcg gtgttgttga catcgaccca aatggccgtg
     2521 ccggcgaagt cgctcagcgt gttgtggcga atctcgaggt cgccgatggc gttggctgtg
     2581 tcggccaggt tgatgaaagc gataccccgg ccgccgccgc tgaacgtcga attctcgatt
     2641 aggccgtcgt gggcattgcc cagcagcact gcctgaacga cattgccggt gaaggtgctg
     2701 ttgtcaatgt gcagcccggt cagatagccg ccgccgaccg actggaacag gccactgtgg
     2761 ccgttgttct cgaaggtcgt atgggtaatg gtcaggccgt tgacgttggt cgtgctggac
     2821 aggcggatgc cgtgggtggg gttgtccttg aagaggctgc cggtaatggt caggttgttg
     2881 agcgtcgtgt tgttgtgcaa ctcgaggcca cggccggcgt tgttcagaaa gcgaacggtg
     2941 tcaaacgaga cgtttgtcag cgtgcccccg tccttccaga cgcgcacggc ccagaggggc
     3001 acgccgccct cgatcggcgc gtcgcgcacg gtcaggtcgc gcacggtgac atcgctggcg
     3061 cgaatctcca ggatgccgtc gcggccggcg acggcctgca ccaatgggtc ataggtcaga
     3121 atcgtcgtcg ccgcgtcggc tcc
//
LOCUS       DAOSMQ010000384         3127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31149_length_3127_cov_4.931966, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000384 DAOSMQ010000000
VERSION     DAOSMQ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31149_length_3127_cov_4.931966"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..93
                     /locus_tag="PK829_11600"
     CDS             13..93
                     /locus_tag="PK829_11600"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOU41901.1"
                     /translation="MRVGDWRVIYEVRNDQLITLVLRIAP"
     gene            150..974
                     /gene="yqeB"
                     /locus_tag="PK829_11605"
     CDS             150..974
                     /gene="yqeB"
                     /locus_tag="PK829_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HOU41902.1"
                     /translation="MLFGDYPILLRGGGDLATGVAYRLHKCGFPVIVTELPRPLVVRR
                     RVSLATAVLEGEVTVEDLRGVRVESAADAWALAMTGAIPVLPSPEWPPAGDARRPAVL
                     IDARMAKRNIDMAIDQAPLVIALGPGFVAGVDCHAVIETNRGHRLGRVLWRGPAEPNT
                     GTPGVVGGKGRERVLRAPVAGVVAWRKEIGDRVREGEPLGDVAGQAIAAPFDGIVRGL
                     IAPGTAVPAALKIGDIDPRDDASAPFTISEKALAIGGGVLEAVLTWMGQEVERYAR"
     gene            964..1902
                     /locus_tag="PK829_11610"
     CDS             964..1902
                     /locus_tag="PK829_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU41903.1"
                     /translation="MRGRRVWMIVGITVLLLVVAYLAAGWYVSQLAMAAPTTSLAEAR
                     AQTGDPADYGLPQPEDVTIDTGEVTLSGWYFDNPAEGNCGVLFMHGFTGTRYQALYWA
                     PLFWAHGCDLLAYDHRGHGDSSPALLTYGYYEKFDALAARNWFAQRAGLEPARIAVGG
                     VSYGAATALQLAPLIPEAPFVLADSSYSSLADILAVRGRVRLGGLATRLLMPGALWIV
                     GLRGGFDVDEVSPEAAIAEATMPILLIHSRTDNFTPYTHSEAIYANSDQSRTVLHIND
                     WGSEHAADIGTDFAAYQALFEDFLTQYAPDFGLPAN"
     gene            1935..2870
                     /locus_tag="PK829_11615"
     CDS             1935..2870
                     /locus_tag="PK829_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU41904.1"
                     /translation="MRRKGLWIVLAVVVILVAVYFGASWYFSEILLNMPTQTIAEARA
                     EGDTPASVGLPEPEEVLIDAGAVTLAGWYFDNPADAGCGLLFLHGFTGTRYHALNWAP
                     LFWERGCDILAYDHRGHGDSSHAFHTYGFYEKQDALVALDWFGRRAGLEQSQIGVFGV
                     SYGAATALQMAPQAPELAFVGADSAYSDMEEIVGYQARRMFPVLAPLLLPGTLAVAEW
                     RADFDVQAVAPEQSVAEAQMPILLIHSLTDEYTPSTHSEDIYAHSNQSRTVLHLTDWG
                     SPHARDILTDYAAYRQLVNDFLEQFAPGFGWPPAS"
     gene            2867..>3127
                     /locus_tag="PK829_11620"
     CDS             2867..>3127
                     /locus_tag="PK829_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41905.1"
                     /translation="MTERQWMLPAALGIGGPELIAIVGGGGKTSLMFALAATLPGRVV
                     VTTTTRIFAAQMRLAPAVVYADDLAPLGPLLEQHGRCLVVGHV"
BASE COUNT          471 a         1085 c         1031 g          540 t
ORIGIN      
        1 cctggctatt gcctgcgtgt cggcgattgg cgtgtcattt atgaagttcg gaacgaccag
       61 ctaatcacac tagtactcag gattgcaccc tgaggagagg cataccgatg agcgtgcagg
      121 tgattctcga tgatctggcc tgataagtaa tgctcttcgg cgactacccc atcctcctcc
      181 gcggcggcgg cgacctggct accggcgtcg cctaccgcct gcacaagtgc ggcttcccgg
      241 tcatcgtcac cgagttgccc cggccgctcg tcgtccggcg gcgcgtgtcg ctggccacgg
      301 ccgtgctgga gggcgaggtg acggttgaag acctgcgcgg cgtgcgcgtc gagtcggccg
      361 cggatgcctg ggcgctggcc atgaccggag cgatccccgt cctgccgtcg cctgaatggc
      421 caccggcggg cgacgcccgc cgaccggccg tcctcatcga cgcgcgcatg gccaagcgca
      481 acattgacat ggccatcgac caggccccgc tggtgatcgc cctggggccg ggcttcgtcg
      541 ccggcgtcga ttgccacgcc gtcatcgaga ccaaccgcgg ccaccgcctc ggccgcgtgc
      601 tctggcgcgg cccggccgag ccgaacacgg gcacgcccgg cgtcgtcggc ggtaaggggc
      661 gcgagcgagt gctgcgcgcg cccgtggccg gcgtcgtcgc ctggcgaaag gagatcggcg
      721 accgcgtgcg cgagggcgag ccgcttggcg atgtggctgg gcaggccatc gccgcgccgt
      781 ttgacggcat cgtccgtggc ctgatcgcgc cggggacggc cgtgcccgcg gcgctgaaga
      841 tcggcgacat cgacccgcgc gacgacgcca gcgccccctt caccatctcg gaaaaggccc
      901 tggccatcgg cggcggcgtt ctggaggcag tattgacctg gatgggtcag gaggtagaaa
      961 ggtatgcgcg gtagacgtgt ttggatgatt gtgggcatca ccgtgctgct actggtcgtg
     1021 gcctacctgg ccgccggctg gtacgtctcc caattggcca tggccgcgcc gacgacctcg
     1081 ctggccgagg cgcgggcgca gacgggcgat ccggccgact acggcctgcc ccaaccggag
     1141 gacgtgacca tcgacaccgg cgaggtcacg ctgagcggtt ggtacttcga caacccggcc
     1201 gagggcaact gcggcgtcct gtttatgcac ggcttcaccg gcacgcgcta ccaggccctc
     1261 tactgggcgc cgctgttctg ggcccacggc tgcgatctgc tggcctatga ccaccgcggc
     1321 cacggcgaca gctcgccggc cctgctgacc tatggctact acgagaagtt cgatgctctg
     1381 gccgcgcgga actggttcgc ccaacgggcc gggctggagc cggcgcgcat tgccgtcggc
     1441 ggcgtgtcct atggcgcggc aacggcgttg caactcgcgc cgctgatccc ggaagcgccc
     1501 tttgtgctgg ccgattcctc ctatagcagc ctggccgaca tcctggcggt gcgcggccgg
     1561 gtgcggctgg gcggcctggc cacgcgcctg ctgatgccgg gggcgctgtg gatcgttggg
     1621 ctgcgcggcg gctttgacgt cgatgaggtt tcgccagagg cggccatcgc cgaggcgacc
     1681 atgcccatcc tgctcatcca ctcgcgcacc gacaacttca cgccctacac ccactccgag
     1741 gccatctacg ccaacagcga ccagagccgc accgtcctgc acatcaacga ctggggctcg
     1801 gagcacgccg ccgacatcgg caccgacttc gccgcttatc aggcgctgtt cgaggacttc
     1861 ctgacccagt acgcgccgga ctttggtctg ccggccaatt gagccataag agaccatcac
     1921 ccgaaggaag cgcgatgcga cgcaaagggt tgtggattgt cctggcggtc gtggtcatcc
     1981 tcgtcgccgt ctactttggg gccagttggt acttctccga aattttgctc aacatgccca
     2041 cgcagaccat cgccgaggcg cgggccgagg gcgatacacc ggccagcgtt ggcttgcccg
     2101 aaccggaaga ggtgctcatc gacgccggcg cggtgacact ggccggctgg tacttcgata
     2161 acccggccga cgctggctgc ggcctgcttt ttctccacgg cttcaccggc acgcgctacc
     2221 acgccctcaa ctgggcgccg ctgttctggg agcgcggctg cgatatcctg gcctacgatc
     2281 atcgcggcca cggcgacagc agccatgctt tccataccta cggcttctat gagaagcaag
     2341 acgctctggt tgcgctggac tggtttggcc ggcgcgccgg cctggagcag tcgcagatcg
     2401 gcgtctttgg cgtgtcctat ggcgcggcca cggccctgca aatggcccca caggccccgg
     2461 agttggcctt cgtcggcgcg gactcggcct acagcgacat ggaggagatc gtcggctatc
     2521 aggcgcggcg gatgtttcca gtcctggccc cgctgctgct gcctggcacg ctggccgtgg
     2581 ccgagtggcg cgccgatttc gacgtgcagg ccgtcgcccc ggagcagagc gtcgccgagg
     2641 cgcaaatgcc catcctgctc atccactccc tgaccgatga gtacactccc tcgacccact
     2701 ccgaagacat ttacgcccac agcaaccaga gccggaccgt gctgcatctg actgactggg
     2761 gttcgcccca cgcgcgcgac atcctgaccg actatgccgc ctaccggcaa ctggttaacg
     2821 acttcctcga gcagttcgcg ccaggcttcg ggtggccacc tgcatcgtga ccgagcgaca
     2881 atggatgttg ccggccgccc tgggcatcgg cggcccggag ttgatcgcca ttgtcggcgg
     2941 cggcggcaaa acaagcctga tgttcgccct ggccgcgacg ctgcccggcc gggtagtggt
     3001 caccacaacc acgcgcatct tcgccgcgca gatgcgactg gccccggctg tcgtctatgc
     3061 tgacgatctt gcgcccctcg gcccactcct ggaacaacac ggccgctgtc tggtcgtcgg
     3121 ccacgtg
//
LOCUS       DAOSMQ010000385         3124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31188_length_3124_cov_3.681004, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000385 DAOSMQ010000000
VERSION     DAOSMQ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31188_length_3124_cov_3.681004"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            618..1478
                     /locus_tag="PK829_11625"
     CDS             618..1478
                     /locus_tag="PK829_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41906.1"
                     /translation="MSATGRTATILHADQEHSGLRMVVFLALFAGLLLGFGVVLLALN
                     AFAPPAIRDYRYFLSCVGAFPVALLLIWGLERLLKRVWHSGLSLELNPYGVIVHDRRG
                     GGARGQGPGSREESGMSAQPSSLAPGTWNLEPTLSWSAHFTQLNWYFRVSGYPRAGRE
                     RRVANKWLCLGTELHQDESRLNVFTFMPPELAAAWIDNPRAAFHRLNPADVYETSPRA
                     RLGPPGRPTIPNQVLHSKDGRYWLAERRRWEYGVELTPQDFNTFMDAALAHARPSPAA
                     GAESPIVSTQ"
     gene            1510..2673
                     /locus_tag="PK829_11630"
     CDS             1510..2673
                     /locus_tag="PK829_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU41907.1"
                     /translation="MFPFITDEHRMIEQAARDFGRNSIAPIAAEHDETGEFPLATIRE
                     MGQLGFMGIEVPEEYGGAGMDTMAYVLAMVEISKADASHGTIMSVNNSLYAHGLLKFG
                     TEEQKQRYLVPVASGQKIGAYSLTEPMSGSDAGTMKSRAVLNPEGTCYVINGRKSWVT
                     SAPYADFIILFTMTQPEKKHKGVTAFIIETDRPGFARGKKEPKLGIRASATSEILFDN
                     YECPVENRLGAEGEGFKIAMTVLDAGRIGIASQALGIAEAAFEASVAYAREREAFGQK
                     IGEFQQIQAKLADMKCRIDASRLLIYQAALAKMAARGGSGRYSLEASEAKLFASETAM
                     WVTTQAIQIHGGMGYSKEMPLERYFRDAKITQIYEGTSEIQRMVIARALTGLR"
     gene            complement(2775..3038)
                     /locus_tag="PK829_11635"
     CDS             complement(2775..3038)
                     /locus_tag="PK829_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOU41908.1"
                     /translation="MNLVSLINALFFIFYVLIFAHIILSWVRPSPYHPVWGPIIRLVN
                     GVIDPIVTPVRRLMPPMGGLDFSPMIILLLARLLQGVLINLVR"
BASE COUNT          593 a         1038 c          988 g          505 t
ORIGIN      
        1 gcttctacat cgatctgatc ggccaggaag gtcgccgacg gctatcacca ccacatcggc
       61 ggcaatacct gggccggggc cggcctgccg cccgccccgg ccgacgcggc gcggctgcta
      121 tggtatgaga ttcgccagcc ggacgcggcg gcgctggagg ccattgccgc gcggctggcg
      181 gcggccggcc gcccctttga gcaggacgcg ggcgggttgc gggtgatgga tccgtcgggg
      241 attgtggtgt tgctgacagc gctttgaatc aggggctgac cgactggtca gcggttgcga
      301 ccggcgtagg aatacgctct aacgtggcta ccatacgtgt atttcgtaac cgacagtcgt
      361 cagttgctcg aagacctgac aggtgggcgg cctgaagttt cctttgcacc agagagagtt
      421 tggccgccca cctgtcaggt aagcgcactt ttagcgactt tggaaaagcc ctgttctcag
      481 ggtaggtcag tggtctggac atctcccggc tctgccgctg ccggagcgca ggcggccacc
      541 gccaaccgca aaaataggat cagtagtggg tcccacaaga cctgtcagtc cgcccgcccc
      601 gtgctacaat cgcctccatg tccgccactg ggcgcaccgc caccatcctc cacgccgacc
      661 aggagcacag cggcctgcga atggtcgtgt tcctggccct ctttgccggc ttgctgctcg
      721 gcttcggcgt cgtcctgctg gcgctgaacg ccttcgcccc gccggcgatc cgggactacc
      781 gctacttcct gtcctgcgtc ggcgcgttcc ccgtggccct gctgctcatc tggggcctgg
      841 agcgcctgct gaagcgcgtc tggcacagcg gcctgagcct ggaactgaac ccctacggcg
      901 tcattgtcca cgatcgacgg gggggagggg ccaggggcca ggggccaggt tccagggaag
      961 aatccggcat gagcgcgcaa ccttcttccc tggcccctgg aacctggaac ctggaaccca
     1021 ccctctcctg gagcgcccac ttcacccagc tcaactggta cttccgcgtc agcggctatc
     1081 cgcgggcggg gcgcgagcgg cgcgtggcca ataagtggct ctgcctggga actgaattgc
     1141 accaggacga gagccgcctc aatgtcttca ccttcatgcc gcccgaactg gccgcggcgt
     1201 ggatcgacaa cccgcgggcg gccttccacc ggctcaaccc ggccgatgtc tacgagacct
     1261 cgccgcgagc gcgcctcggc ccgcccggcc gcccgaccat ccccaaccag gtgctccact
     1321 cgaaagacgg ccgctactgg ctggccgagc gccgccgctg ggaatatggc gtggagttga
     1381 cgccgcaaga tttcaatacc ttcatggacg ccgccctggc acacgcccgg cccagcccgg
     1441 ccgccggcgc ggagtctccc atcgtctcaa cacagtagcc gccgacgcgc ggcccaggag
     1501 agttgtgcaa tgttcccctt catcaccgac gagcaccgca tgatcgaaca ggcggcgcgc
     1561 gacttcggcc gtaattccat cgcccccatc gccgccgagc acgacgagac gggcgagttc
     1621 ccgctggcca ccatccgcga gatgggccag ttgggcttca tgggcatcga agtgccggag
     1681 gagtatggcg gcgcgggcat ggacaccatg gcctacgtgc tggccatggt cgagatcagc
     1741 aaggccgacg ccagccacgg cacgatcatg tcggtcaaca attcactcta cgcccacggc
     1801 ctgctcaagt ttggcacgga agagcaaaag cagagatacc tcgtgcccgt ggccagcggg
     1861 cagaagatcg gcgcctatag cctgaccgag ccgatgagcg gcagcgacgc gggcacgatg
     1921 aaaagccgcg ccgtgctgaa ccccgaaggc acatgttacg tcatcaatgg ccgcaagtcg
     1981 tgggtcacct ctgctcccta tgccgacttc atcatcctct tcaccatgac ccagccggag
     2041 aagaagcaca agggcgtgac ggcgttcatc atcgagaccg accggcccgg cttcgcgcgc
     2101 ggcaagaagg agccgaagct gggcatccgc gccagcgcca ccagcgagat tctcttcgac
     2161 aactacgagt gcccggtgga gaaccgcctc ggcgcggagg gcgagggctt caagatcgcc
     2221 atgaccgtcc tcgacgccgg gcgcattggc atcgcctcgc aggcgctggg catcgccgag
     2281 gcggccttcg aggccagcgt cgcctacgcc agggagcgcg aggcgttcgg ccagaagatc
     2341 ggcgaattcc agcagattca ggccaagctg gccgacatga agtgccgcat cgacgccagc
     2401 cggctgctca tctaccaggc ggcgctggcc aagatggccg ccaggggtgg cagcggccgc
     2461 tactccctgg aggccagcga ggccaagctc ttcgccagcg agacggccat gtgggtgacg
     2521 acccaggcca tccagatcca cggcggcatg ggctatagca aggagatgcc gctggagcgc
     2581 tacttccgcg acgccaagat cacccagatc tatgagggca ccagcgagat tcagcgcatg
     2641 gtcattgccc gcgcgctgac gggactgaga tagggaccag gggccaggga ttaggggcta
     2701 gggattaggt tctagggact agggaagagc gcccttccct agtccctaaa acctaaaacc
     2761 tagcccctat tctcctagcg gactagattg atcagcaccc cctgcagcag ccgcgccagc
     2821 agcagaatga tcatcgggct gaagtccagg ccacccatcg gcggcatcaa ccggcgcacg
     2881 ggcgtgacaa tcgggtcaat gacgccatta acgaggcgga taatcggccc ccagacgggg
     2941 tggtaaggac tggggcgcac ccacgatagg atgatgtggg cgaatatcag cacgtagaag
     3001 ataaagaaga gagcattaat cagactgacc aggttcattt cacctcagag tttcgcttca
     3061 cgttcgccaa acagggcgcg accaacgcgg acgtgggtcg ccccttcctc gatggcgatc
     3121 tcgt
//
LOCUS       DAOSMQ010000386         3099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31516_length_3099_cov_4.080486, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000386 DAOSMQ010000000
VERSION     DAOSMQ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31516_length_3099_cov_4.080486"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PK829_11640"
     CDS             <1..237
                     /locus_tag="PK829_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate phytyltransferase"
                     /protein_id="HOU41909.1"
                     /translation="GRALLTALYLLPIGALLARPGPAAAALIAVRVALLAVFWLISRR
                     TDPARPPSMARLYFVLWGLFYAEYLLLALGGAGR"
     gene            complement(280..2163)
                     /locus_tag="PK829_11645"
     CDS             complement(280..2163)
                     /locus_tag="PK829_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41910.1"
                     /translation="MQSLLSAVLVILIIFVLLIIFVPAWLERRARAHAVLREQFAADI
                     QAQERAARRLELSLSPYARGRSAHFVARVDAARERLASVRVLVGDAAATLDALRLPVV
                     YKYLLPVQHFALVPRDAGAILADTRAVRRLRGQLTAVAAAAAAAEAAVGELTALPQTL
                     AEERRALAAQLDGVDATLRAERAAGIERLSDLEGELNRLRALLARQQTCGDGPPAMLD
                     DGAAALEEAAPGATALAGQVAEVAAARVRLDERLRAVATDLDDIQAGAKATPAAAPSQ
                     VRSPLRRAAALLNESAAEHRRARDFAAAEADIAAAARLVVLARDAAAAAAHGRLLADR
                     DDGVSLAAPIADLRRELGELLDGLENRLGSASREATLVARAGDIRRRAEALLSRQDEV
                     IAALDRDASAVQARLAHGWEAARQLLPLAADDPLARRYAALAAATDEARRSPTALEAY
                     RRDAAAFEAEMAPWVTRVQAGRQLVAELRARLPQQIDAAIQLAAPWACLQEHVAFIHQ
                     RALDFESAQSQLARASRRREAEPPLDELADIDSQVAERMTLLGYQADRLRFLETGLGE
                     IIALAEADSEGLPPDDPALVRRNKAIRLIAQYSKQAHAAQKYEDASLALSRAADVANR
                     AAL"
     gene            2364..2693
                     /locus_tag="PK829_11650"
     CDS             2364..2693
                     /locus_tag="PK829_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU41911.1"
                     /translation="MDTFHALADPNRRQIMEMLAREGELSATEIHSRFSVSAPAVSQH
                     LKVLREAGLVHMEKRAQQRIYRVAPEAMREVEDWASAIRNQWARRFDALDALLKEEGA
                     KPPKEQE"
     gene            2693..>3099
                     /locus_tag="PK829_11655"
     CDS             2693..>3099
                     /locus_tag="PK829_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOU41912.1"
                     /translation="MNETQYLISERIFDAPREAVWRAWTDPEWLMRWWGPEHFTSPAA
                     RMDVRPGGRYLWGMRDPGGHDYWSTGVFREVVPLERLVYTDSFSDEHGNVVSPAAYGM
                     GDDFPAESTITVLLEDLGGRTRMRVYTDQKPDG"
BASE COUNT          465 a         1106 c         1084 g          444 t
ORIGIN      
        1 ggccgcgccc tgctgacggc gctctacctg ctgcccatcg gcgcgctgct ggcccggccc
       61 ggcccggcgg ccgcggcgct catcgccgtc cgcgtcgccc tgctggccgt cttctggctc
      121 atcagccgcc gcaccgaccc ggctcgcccg ccatccatgg cgcggttgta ctttgtcctg
      181 tgggggctgt tctatgcgga gtatctgctg ttggcgctgg ggggggccgg ccgctaacga
      241 ctgacccact gctcccctgc cccttcccgt cctcaacgcc tacagcgccg cccggttggc
      301 cacatcggcg gcgcgcgaaa gggccaacga ggcatcctcg tacttctgcg ccgcgtgggc
      361 ctgcttggag tactgggcaa tcaggcgaat ggccttattg cgccggacga gggccgggtc
      421 atcgggcggc aacccttcgc tgtcggcctc ggccagggcg atgatctcgc ctagccccgt
      481 ctccagaaag cgcagccggt cggcctggta gccgagcaag gtcatacgtt cggccacctg
      541 gctgtcgatg tctgccaact catcgagggg cggctcggcc tcgcgacgac ggctggccct
      601 ggccagttgg ctttgcgccg actcgaaatc gagcgcgcgc tgatggatga aagcgacatg
      661 ctcctgcaag caggcccacg gcgcggccaa ctggatggca gcgtcgatct gctgcggcag
      721 gcgggcgcgc aactcggcca caagctgccg cccggcctgc acccgtgtca cccagggagc
      781 catctcggcc tcaaacgcgg ccgcgtcgcg ccggtaggcc tccagcgccg tcgggctgcg
      841 gcgggcctcg tcggtggcgg cggccagggc ggcgtagcgg cgggccaacg ggtcgtcggc
      901 cgccagcggc agcagctgcc gggccgcctc ccaaccgtga gccagacggg cctgaacggc
      961 cgacgcgtcg cgatccagcg cggcgatcac ctcatcctgc cgcgagagca gcgcttcggc
     1021 ccggcggcga atgtcgccgg cgcgggccac cagggtggcc tcgcgcgagg cgctgccaag
     1081 ccggttctcc agcccgtcga gcaattcgcc caactcgcgg cgcaggtcag cgatgggcgc
     1141 ggccaggctg acgccatcgt cgcggtcggc cagcaaccgg ccgtgggccg cggcagcggc
     1201 ggcatcgcgg gccaggacta ccagtcgcgc cgccgcggcg atgtcggcct cggcggcggc
     1261 gaaatcgcgg gcgcggcggt gctcggcggc gctctcgttc agcagcgccg ccgcccggcg
     1321 caacggcgac cgcacctgag agggggcggc cgccggcgtg gccttggccc cggcctggat
     1381 atcatccagg tcggtggcca cggcccgcaa gcgctcgtcc aggcgcacgc gggccgcggc
     1441 gacctcggcc acctgcccgg ccagcgccgt cgctccgggg gcggcctcct ccagcgccgc
     1501 ggcgccatcg tccagcatcg ccggcgggcc gtcgccacag gtctgttgcc gggccagcag
     1561 cgcgcgcagc cggttgagtt cgccttccag gtcgcttagc cgctcaatgc cggcggcccg
     1621 ttcggcgcgc agagtggcgt cgacgccgtc gagttgcgcg gccagagcgc ggcgctcctc
     1681 ggccaacgtc tgcggcagcg ccgtcagctc gcccaccgcc gcctcggcgg cggccgcggc
     1741 cgcggccacg gccgtcagtt ggccgcgcag ccggcgaacg gcccgcgtgt cggccaggat
     1801 ggccccggcg tcgcgcggca cgagggcaaa gtgttggacg ggcagcaaat acttgtagac
     1861 cacgggcagg cgcagcgcgt ccagcgtcgc cgccgcgtcg ccgacgagga cccgcaccga
     1921 ggccagccgc tcgcgggccg cgtcgacgcg ggcgacaaag tgggccgaac gcccgcgggc
     1981 ataggggctg agcgacagct ccaaacgccg cgcggcgcgc tcctgggcct gaatgtcggc
     2041 ggcgaattgc tcgcgcagaa cggcatgggc ccgcgcccgg cgctccagcc acgccggcac
     2101 gaagatgatc agcagcacaa aaatgatcag gatgaccagg acggccgaaa gcagcgattg
     2161 cacgtcgatc tcctttaact tctattatag tggaaaagtg gccggtgggc agtggggagt
     2221 agcggtcatt gacctgacag atgggcggcc cgccgttctc cggtgaaagg ctcatgcgtg
     2281 gccgcctacc tgtcaggtcg gcgcgccgcc ggccactaat ttaagtattg acttatttaa
     2341 gtgaatcatt tataatgctg cgcatggata cattccacgc cctggccgac cccaatcgtc
     2401 gccaaatcat ggagatgctc gcgcgcgagg gcgaactatc ggcgaccgag attcacagcc
     2461 gcttctcggt cagcgcaccg gccgtttccc agcacctcaa ggtgttgcgc gaggccggcc
     2521 tggtgcacat ggagaagcgc gcccaacagc gcatctaccg cgtcgccccg gaggccatgc
     2581 gcgaggtcga agactgggcc agcgccatcc gcaaccagtg ggccaggcgc ttcgacgccc
     2641 tggacgcgct tctgaaggaa gaaggggcca agccgcccaa ggaacaagag tgatgaacga
     2701 gacacaatac ctgatcagtg aacgcatttt cgatgctccg cgcgaagccg tctggcgggc
     2761 ctggaccgac ccggagtggc tgatgcgctg gtgggggccg gagcatttca cctcgcccgc
     2821 cgcccgtatg gacgtgcggc cgggcggtcg ctatctctgg ggcatgcgcg accccggcgg
     2881 ccacgactac tggtcaaccg gcgtctttcg ggaggtcgtt ccgctggagc ggctggtcta
     2941 caccgacagc ttcagcgacg aacacggcaa cgtcgtctcg cccgcggcct acggcatggg
     3001 cgacgatttc ccggccgaat cgaccattac cgtgctgctg gaagacctgg gcggccggac
     3061 gcggatgcgc gtctacaccg accagaagcc cgacggcga
//
LOCUS       DAOSMQ010000387         3098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31523_length_3098_cov_5.095301, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000387 DAOSMQ010000000
VERSION     DAOSMQ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31523_length_3098_cov_5.095301"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1071
                     /gene="holB"
                     /locus_tag="PK829_11660"
     CDS             52..1071
                     /gene="holB"
                     /locus_tag="PK829_11660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOU41913.1"
                     /translation="MLPLASGWKAIIGHEWAVRLLSNAVAHGRAGHAYLFTGPDGIGK
                     MTLARTFAATLNCAAPAGERPCGQCRSCQLIAEDKHPDVRVVVPEVSERGTPSIKIEA
                     IRRLQSDLSLSAYEGRYKIALLRRFDTANPNAANAFLKTLEEPPAHVILLLTAADSDS
                     VLPTINSRCRTMGLRPLATPLVEEALLTHFDASPDEANLLAHLADGRLGWAISAHRQP
                     AALHDREAQLATLDQALSGSLVARFALAETLARKTDTLPALLRTWLSWWRDMALLAFG
                     PHRDDAISNIDRVEQLHHLAAQWPRPQVLAALRQTEAALRQLAQNGNARLVLENVLLI
                     YPRIE"
     gene            1071..1628
                     /locus_tag="PK829_11665"
     CDS             1071..1628
                     /locus_tag="PK829_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41914.1"
                     /translation="MTPQSSPERFTISATPSAGVGALLLAIVFGFVGRRLFRLSPGEA
                     VAGGVLAAALHFLSELWHQKGHARAAARAGYPMTGVRLWGVLGTSLYPPDEPALPDEV
                     HVARALGGPRASAWLAGGGALAAVLAWPVGGVARMVSTLFALENLLVFTLGAFLPMPF
                     METDGATILHHRQTAGGSRRIVIQE"
     gene            1637..2434
                     /locus_tag="PK829_11670"
     CDS             1637..2434
                     /locus_tag="PK829_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOU41915.1"
                     /translation="MSIEADLATLLAFALDAAWQAGRITLGHFQTGLAAERKADNSPV
                     TIADRQAEQKLRDLIMTAWPDHALIGEEYGHQPGRRDSGYTWIIDPIDGTKSFISGVP
                     FYGVLLALVKGEQPLLGVTYFPALDEMVYATRGGGCYWNGRRAHVSAVARLADAVLLA
                     SDLNTFAQFNRQAPFQRLIDATYYQRTWGDAYGYALVATGRAEVMLDPVMALWDCGPL
                     QVILEEAGGTFTDWRGTPTIFAGEAVATNGTLFAPVMELVRGGMEQA"
     gene            2548..>3098
                     /locus_tag="PK829_11675"
     CDS             2548..>3098
                     /locus_tag="PK829_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41916.1"
                     /translation="MGQTSDSAPSLDTGPEQRPRRPFFRLPGPDLTAGAGEHSGPRAR
                     GLPWRGAARELAPIGLLALVAVVFFAPVVAGRAWLPKGGGDSVSFLFPMYRFAADSLR
                     AGTIPFWNPHQYAGAPFLADNQSGIFYPFNLLLFFLWPAFSYRGVEALVVWHFFFAGL
                     ATYICLRRLQPEVVVGRAAALLGA"
BASE COUNT          443 a         1120 c          997 g          538 t
ORIGIN      
        1 gggagagggg cgtttctttc ctgcatttcc atgacaggtt acaattctca catgctcccc
       61 cttgcttctg gctggaaggc gatcatcggc catgaatggg ctgtccgctt gctgagcaac
      121 gccgtggccc acggccgcgc cggccacgcc tacctgttca ccggccccga tggcattggc
      181 aagatgaccc tggcgcggac gttcgccgcg acgctcaatt gcgccgcccc ggccggcgag
      241 cggccctgtg ggcagtgccg cagttgccag ctcatcgccg aggataagca ccccgacgtg
      301 cgcgtcgtcg tgcccgaagt cagcgagcgc ggcacgccat cgatcaagat cgaagccatc
      361 cgtcgcttac agagtgacct gagcctgtcg gcctatgagg ggcgttacaa gatcgccctg
      421 ctacggcgct tcgatacggc caaccccaat gccgccaacg ccttcctgaa gacgctggag
      481 gagccgccgg cccatgtgat cttgctgctg acggccgccg acagcgacag cgtcctgccg
      541 acgatcaact ctcgctgccg gacaatgggg ttgcggccgt tggccacgcc actggtcgaa
      601 gaggcgctgc tcacccactt cgacgccagc cccgacgagg ccaacctgct tgcccacctg
      661 gccgacggcc ggctgggctg ggccatcagc gcccaccggc agccggcggc gctgcacgac
      721 cgcgaggcgc aactggcgac gctcgaccag gcgctgagcg gctcgctggt ggctcgcttc
      781 gccctggccg agacgctggc gcgcaagacg gatacgctgc ccgccctgct gcgcacgtgg
      841 ctgagctggt ggcgcgacat ggccctgctc gctttcggcc ctcaccgcga tgacgccatc
      901 agcaacatcg accgggtgga acaactccac cacctggcgg cgcagtggcc acggccgcag
      961 gtcttggccg cgctgcgcca gaccgaagcg gcccttcgcc aactggccca gaacggcaat
     1021 gcccgcctgg tgctggaaaa tgtcctgttg atctatccgc gaatcgaatg atgacgccac
     1081 aatcttcgcc ggaacggttc acgatcagcg cgaccccctc cgcgggcgtt ggcgcgctcc
     1141 tgcttgccat tgtcttcggc ttcgtcggcc ggcgactgtt tcgcctgtcg cccggtgagg
     1201 cggtggccgg cggggtgctg gcggcggcac tgcactttct gtccgagttg tggcaccaga
     1261 agggccacgc ccgcgccgcc gcgcgcgccg gctaccccat gaccggcgtg cggctgtggg
     1321 gcgtgctggg cacgtcgctc tacccgcccg acgagccggc gctacccgat gaggtccacg
     1381 tcgcccgcgc cctgggtggc ccgcgggcca gcgcctggct ggccggcggg ggagcgctgg
     1441 cggctgtact ggcctggccc gttggcggcg tcgcccggat ggtcagcact ctgttcgcgc
     1501 tagaaaacct gctcgtcttc accctgggcg cgttcctgcc catgcccttc atggagacgg
     1561 acggggcaac catcctccac caccgccaga cagccggggg cagccgccgg atcgtcattc
     1621 aggaatagca tccgccatgt ctatcgaagc cgatctcgcc acgctactcg cctttgccct
     1681 cgatgccgcc tggcaggctg ggcggatcac tctgggtcat ttccagaccg gcctggccgc
     1741 cgagcgcaag gccgacaaca gccccgtcac catcgccgac cggcaggccg agcaaaagct
     1801 gcgcgacctc atcatgaccg cctggcccga ccacgccctc attggcgagg agtatggcca
     1861 ccagcccggc cgccgcgaca gcggctacac ctggatcatc gaccccattg acggcacgaa
     1921 gtcgttcatc agcggcgtgc ccttctacgg cgtgctgctg gcgctggtca agggggagca
     1981 accgctgctg ggcgtgactt acttccccgc cctggatgag atggtctacg ccacgcgggg
     2041 cggtggctgc tattggaacg gccgccgggc gcacgtctcg gccgtcgccc gtctggccga
     2101 tgccgtcctg ctggccagcg acctcaacac cttcgcccag ttcaaccgac aggccccttt
     2161 ccagcggctc atcgacgcca cctactatca acgcacctgg ggcgacgcct atggctacgc
     2221 cctggtggcc accggccggg ccgaggtgat gctcgacccg gtcatggccc tgtgggactg
     2281 cggcccgttg caagtcatcc tggaggaggc tggcggcacc ttcaccgact ggcgcggcac
     2341 gccgaccatc ttcgccggcg aagccgtagc caccaatggg acgttgtttg cgccggtgat
     2401 ggagttagtg cggggtggga tggaacaggc atagataggg gctattgtag gggcgggtct
     2461 gagacccgcc cctacagggt gtgtgccgct ctcttgccca tggtggcggc ggcgcgccgg
     2521 cctgtgctac aatcggcgat tgacattatg gggcaaacct ccgatagtgc gccgtcgctc
     2581 gacaccggtc cagagcagcg tccgcgacga ccgttcttcc gtctgcccgg ccctgacttg
     2641 accgcggggg ccggcgagca ctccggcccc cgcgcccgcg gcctaccctg gcgtggcgcg
     2701 gcacgcgagc ttgcccccat tggcttgctg gccctggtcg cggtggtctt cttcgcgccg
     2761 gtggttgccg gccgggcctg gctgcccaag ggcggcggcg attccgtctc cttcctgttc
     2821 cccatgtacc gcttcgccgc cgacagcctg cgcgccggca caatcccctt ctggaacccg
     2881 caccagtacg ccggcgcgcc ctttctggcc gacaaccaat caggcatctt ctacccattc
     2941 aacctgctgc ttttcttcct ctggccggcc ttctcctatc gcggcgtcga agcgctggtc
     3001 gtctggcatt tcttcttcgc cgggctggcc acctatatct gcctgcggcg gttgcagccg
     3061 gaagtggtcg tcggccgggc cgcggcgctg ctgggcgc
//
LOCUS       DAOSMQ010000388         3089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31643_length_3089_cov_4.534937, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000388 DAOSMQ010000000
VERSION     DAOSMQ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31643_length_3089_cov_4.534937"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK829_11680"
     CDS             complement(<1..200)
                     /locus_tag="PK829_11680"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOU41917.1"
                     /translation="MQTQITCPNCGVPYVADIHQVIDAARQPQLKEMLLSGQLNVAVC
                     PNCGAGGRVATPLLYHDPDHDL"
     gene            complement(280..1887)
                     /locus_tag="PK829_11685"
     CDS             complement(280..1887)
                     /locus_tag="PK829_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU41918.1"
                     /translation="MTRALVDIRILQREYLLGISRAMTAELDLHDLLRLILKSAVELV
                     SGQAGMIALAEPGSERLRVAAVYGIPPHLVDHFAPLMRGLPYREGHDGEAFPELVEQL
                     RRVAKEANLGLSQVIRLPLSSGGIVIGLIYVFQSGNYYFVEDAPNLLRSFAEQAAIAV
                     GNARLYQRVNQEKQRLDAILEQSADGVMILDPQLKITVFNRALSHMTGWPAAEAIGRS
                     HADVIQWQTLKTESDLNAALARNWPQPNAAHLYVEGEARRRRDAACPTERDTLSLGIT
                     YAPLLGADGQMTDIIANVRDLTRYREEEALQKTFISVISHELKTPVSIIKGYAGTLRR
                     PEGRWSPDVLDESLAVIEEEADNLNGLIDNLLEASRLQAGTFTLEISDEVSLPRLAAA
                     VARKFTTQTKTHTIAVDFPADFPTVIGDERRLTQVLNNLVSNAIKYSPDGGRIDIAGE
                     VYPQHVTVSVCDEGIGIAARDQHRIFQKFSRVDNALSRKTEGTGLGLYLSRAIVEAHG
                     GRIWFRGNSEGDAGAPGTTFTFSLPRD"
     gene            2058..3014
                     /locus_tag="PK829_11690"
     CDS             2058..3014
                     /locus_tag="PK829_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOU41919.1"
                     /translation="MDDQQMIPFAGVRLGHATHPEYPTGCTVFLCPAGTFGSVEARGP
                     APGSRELAVLAADKPDDKEVNAVVLTGGSAFGLATADGVMRYLAERGIGHPTPIRPVP
                     IVPAAVVFDLWPGRTFTPDAAMGYAACEAATGYEGEIDQGNVGVGAGVLVGKWAGFQY
                     MMKGGFGVASVREAGVEVAAAAVVNAVGDIVNADGTVLAGARDPAGGWLAERNPLRFV
                     DEARLPLPGTNTTLVVVATDAILTRNELARLTHQAHNGLAIAVRPSHTRHDGDVAFAL
                     STRRVAAPVTLVGNMAVAAVAEAIRNAARFAASIPGVPGLAG"
BASE COUNT          487 a          961 c         1057 g          584 t
ORIGIN      
        1 aacaggtcgt ggtcggggtc gtggtagagc aggggcgtag cgacacggcc gcccgcgcca
       61 cagttggggc agacggcgac attgagctgg ccggacagca acatctcttt aagctgtggc
      121 tggcgcgcgg cgtcaatgac ctgatggatg tcggcgacgt agggtacgcc gcaattgggg
      181 caggtaatct gcgtttgcat tccgtctcct tgatcggcgc ggggtggcat agggcgggca
      241 tgtgccgcgc ttcagccgtc cgcgcgaagt gtccttcggt taatctcgcg gcagcgagaa
      301 ggtaaatgtc gttcccggcg cgccggcgtc gccttcgctg ttgccgcgga accagatgcg
      361 gccgccatgc gcctcaacga tggcccgtga cagatacagc cccagtcccg tgccctctgt
      421 cttgcgactg agagcgttat cgacgcggga gaacttctgg aagatacgat gctggtcgcg
      481 ggcggcaatg ccgatgccct cgtcgcagac cgatacagtg acgtgctgcg ggtacacctc
      541 gccagcgatg tcaatccggc cgccgtcggg cgagtacttg atggcgttgc tgaccagatt
      601 gttgagcacc tgtgtcagcc gccgctcgtc gccgatgacg gtcggaaagt cggccgggaa
      661 atcgacggcg atggtgtggg tcttcgtctg cgttgtgaac ttacgggcta cggccgcggc
      721 cagccgcggc aagctgactt catcgctgat ctccagcgtg aaggtgcccg cctgcaagcg
      781 cgaggcctcc aggaggttgt cgatcagccc gttgaggtta tcggcctcct cttcgatgac
      841 ggccaacgac tcgtcgagca cgtctggcga ccagcggccc tccggccgtc gtaatgtgcc
      901 ggcatagccc ttgatgatcg acaccggtgt ctttaactcg tggctgatga ccgagatgaa
      961 tgtcttctgc agggcctctt cctcgcgata gcgggtcagg tcgcggacgt tggcgatgat
     1021 gtcggtcatt tggccgtcgg cgccgagcag cggggcgtag gtgatgccca ggctgagtgt
     1081 gtcgcgctcg gtggggcagg cagcgtcgcg gcggcggcgc gcctcgcctt cgacgtagag
     1141 atgggcggca ttgggttggg gccagttgcg ggccaacgcg gcgttcaagt ccgactccgt
     1201 tttcaacgtt tgccactgga tgacgtccgc gtggctgcgg ccgatggcct cagccgccgg
     1261 ccagccggtc atgtggctca gggcgcggtt aaagacggtg atcttcagtt gtgggtcaag
     1321 gatcatcacg ccgtcggcgc tttgctccag gatcgcgtcc agacgctgct tttcctgatt
     1381 gacgcgctgg tatagccggg cattgccaac ggcgatagcc gcttgctcgg cgaagctgcg
     1441 cagtagattg ggcgcgtctt cgacgaagta atagttgccg gactgaaaga cgtagatcag
     1501 gccaatgacg atgccgccgc tgctgagcgg caggcggatg acctggctca ggccgaggtt
     1561 ggcctcctta gccacgcgcc gtagctgctc caccagttcg ggaaacgcct cgccatcgtg
     1621 gccctcgcga tagggcaagc cacgcatcag cggcgcgaag tgatcaacca ggtggggggg
     1681 gatgccgtag acggcggcga cgcgtaagcg ttcgctgccc ggttcggcca gggcgatcat
     1741 acccgcctgt ccggagacga gttcgacggc cgacttgagg attagccgta gcaggtcatg
     1801 caggtctaac tcggccgtca tcgcccgact gatgcccagc aagtactcgc gctgtaagat
     1861 acggatatcg accagggccc ttgtcatgct tttctttccg agacaacgac actcgattct
     1921 agcacacgtt gccacggcga ctatcagcat agcggttttc tcttggaatt gcgtaatagc
     1981 agtgctagag ttgtggcaga tttggcccat ttcgcggcct ggccggcaaa cacgcaggcg
     2041 accagacgag gtgggcaatg gatgaccaac agatgatccc cttcgccggc gtgcgcctgg
     2101 gccacgccac acaccctgag tatcccaccg gttgcacggt cttcctctgt ccggcgggca
     2161 cgttcggcag cgttgaggcg cgtggcccag cccccggcag ccgcgagttg gccgtgctgg
     2221 ctgccgacaa gccggatgac aaggaagtca acgccgtggt gctgactggc ggcagcgcct
     2281 ttgggctggc cacggccgac ggcgtgatgc gctatctggc cgagcgcggc atcggccacc
     2341 cgacgcccat ccgccccgtg cccattgtgc cggcggcggt cgtctttgac ctctggccgg
     2401 gtcgcacctt cacgcccgac gcggccatgg gctacgcggc ctgcgaggcc gccactggct
     2461 atgagggtga aatcgatcag ggcaatgtcg gcgttggggc gggcgtgctg gtgggcaagt
     2521 gggccggctt ccagtacatg atgaaaggtg gctttggcgt ggcctcggtg cgcgaggcgg
     2581 gcgtcgaagt ggccgcggcg gcggtggtca acgccgtcgg cgacatcgtc aacgccgacg
     2641 gtaccgtgct ggccggcgcg cgcgacccgg ccggcggctg gctggccgag cgcaacccgt
     2701 tgcgcttcgt ggacgaagcc cgcttgccgc tgccgggcac gaacacgacg ctggtggtcg
     2761 tggccaccga cgccatcctg acacgcaacg agctggcccg gctgacccac caggcccaca
     2821 acggtctggc tatcgccgtg cggccgtcgc acacccgcca cgacggcgat gtggctttcg
     2881 ctctgtccac ccgtcgcgtc gcggccccgg tcacgttggt cggcaatatg gccgtcgccg
     2941 ccgtggcgga ggccattcgc aacgccgcgc gcttcgccgc gtccatccct ggcgtgcccg
     3001 gcctggccgg ctgagcgctt cgtctattgg atgtgtctat gaaccaggtc atctctgtgt
     3061 ttggaagttc ctcgcccacc ccggatagc
//
LOCUS       DAOSMQ010000389         3076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31796_length_3076_cov_4.770606, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000389 DAOSMQ010000000
VERSION     DAOSMQ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31796_length_3076_cov_4.770606"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..998
                     /gene="minC"
                     /locus_tag="PK829_11695"
     CDS             288..998
                     /gene="minC"
                     /locus_tag="PK829_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOU41920.1"
                     /translation="MTPAVDIKGIRDGLLIRVSDRANVPLAEALGDELARKRAFLAGS
                     RVAVDVGGRALGGEQLAALGQLFAAHDMTLWAVLAADGDTRDAVRRAGLATRLSGSAT
                     DLDGNALAAPPNGGEPPAKEPPPANALLLRETVRSGRAIWHEGHVVIVGDVNPGAEVM
                     AAGSVIVWGRLRGLVHAGALGDTSAVICALELNPTQLRIADHIAIAPDNGPGRSPEQA
                     AIRDGQIVAEPWVTRPTS"
     gene            1072..1878
                     /gene="minD"
                     /locus_tag="PK829_11700"
     CDS             1072..1878
                     /gene="minD"
                     /locus_tag="PK829_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOU41921.1"
                     /translation="MSAQVLTVTSGKGGVGKTTVTANLATALAMLGKRVTAIDADIGL
                     RNLDVVMGLENRIVYDLVDIVEGRCRLRQAMIKDKRQPELYLIPAAQTRDKTAVSPSD
                     MVLLCDQLRQEMDYIVIDSPAGIERGFRNAVAPADKVLIVTNPEVSAVRDADRIIGLI
                     EAEEKGPGQLIVNRVKPQMVSRGDMLSVDDVLDILAVELLGVIPEDERILVASNQGLP
                     LVMHGLNGSSAGEAFRHVARRLDGESVPFMDLSVKPTFMSRLRAFMNGQN"
     gene            1892..2158
                     /gene="minE"
                     /locus_tag="PK829_11705"
     CDS             1892..2158
                     /gene="minE"
                     /locus_tag="PK829_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOU41922.1"
                     /translation="MSWLNRLLGKNETSGQIAKQRLQMVLTHDRADLPPGLLEQIKDD
                     IIEVIARHTAVDTVNVAVHLEQNARENRLIAEVPLLAARRGTRG"
     gene            2294..>3076
                     /locus_tag="PK829_11710"
     CDS             2294..>3076
                     /locus_tag="PK829_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU41923.1"
                     /translation="MLSKTSVWRYFDVWLLAAVLLLTAYGILMIRSAITGAPNFAGYP
                     QQQLIWAILGLIILLVVAAIDYRVLTSAHWYIYGGLVLLLVAVIVTGQVNNEARRWIQ
                     ITPSFQIQPSEFGRIFITVTFAQFLANRRHLIGRFSNTLLTLAYFALPVGLIFIQPNL
                     GMTLLFMFIWFIMLWLAGLPLRHFAALLFLGVGLGIAIFPLLADYQKDRVSTFLNPEA
                     ANEDDVFNVDQAEISIGSGGWLGKGYLQGTQSQLGFLRAQHTD"
BASE COUNT          548 a         1009 c          974 g          545 t
ORIGIN      
        1 cgaccgccgc gcccacagcc gtctgaagac catcggccgc gagcacgtct gccccgactg
       61 cctacgcgtc ctcgaccgtc agccccagcc ctgagtagcc caccgcgggg cgcgcctgct
      121 cagggcatat atctggccct ccctctctgt aaacggctat acatggccgc tgtcatggcg
      181 gctacttgcc atgatgttga acttgttgta tcatttcaac aatactgcaa gcgtatggat
      241 ggcacagtga tggttagtcg agcgtaatgg caggcgacga gaagcgcgtg acaccggcgg
      301 ttgacatcaa aggtattcgc gacggcctgc tgattcgggt cagcgaccgg gccaacgtac
      361 cattggccga ggcgctgggc gacgaattgg cccgcaagcg ggcctttctg gccggcagcc
      421 gggtggccgt cgatgtgggc ggccgggcgc tgggcggcga gcagttggcg gcgttgggcc
      481 agttgttcgc ggcccacgac atgacgctgt gggccgtgct ggccgcagac ggcgatacgc
      541 gcgatgctgt ccgccgcgcc gggctggcga cgcgcctgtc cggcagcgcc actgacctgg
      601 acggcaatgc cctggccgcg ccgcccaacg gcggcgagcc gccggccaaa gagccgccgc
      661 cggccaacgc cctgctgctg cgcgagacgg tgcgctccgg ccgggctatc tggcacgaag
      721 ggcacgtcgt catcgtcggc gatgtcaacc ctggcgcgga ggtcatggcc gcgggcagcg
      781 tcatcgtctg ggggcggctg cgcggtctgg ttcacgccgg cgcgctgggc gacaccagcg
      841 cggtcatttg cgccctggag ttgaacccga cccagctacg catcgccgac cacatcgcca
      901 tcgccccgga caacggtccc ggccgctccc cggagcaggc ggccatccgc gacgggcaga
      961 tcgtcgccga accgtgggtg acccggccaa cgagttgagc gtaactgcca tcaagaagtg
     1021 tgcagtgatg gtagcgcctt ttcggcagga atgaacagaa ggtcgaatcc tatgagtgca
     1081 caagtgctga cagttacatc gggcaagggc ggcgtcggca agacgaccgt gaccgccaac
     1141 ctggccacgg cgctggctat gctgggcaag cgggtaacgg ccatcgacgc cgacatcggc
     1201 ctgcgcaatc tcgacgtggt catggggctg gagaaccgca tcgtctatga cctcgttgac
     1261 attgtggagg gtcgctgccg gctgcgccag gcgatgatca aggacaagcg ccagccggag
     1321 ctatacctta tccccgcggc ccagacgcgc gacaaaacag ccgtcagccc gtcggacatg
     1381 gtcctgctgt gcgaccagtt gcgccaggag atggactaca tcgtcatcga ctcgccggca
     1441 ggcatcgagc gcggcttccg caacgccgtg gccccggccg acaaggtgct catcgtcacc
     1501 aacccggagg tgtcggccgt gcgcgacgct gaccgcatca tcggcctcat cgaagccgag
     1561 gagaaagggc cggggcaatt gatcgtcaac cgcgtcaagc cgcagatggt cagccgcggc
     1621 gatatgctct cggtggacga tgtcctcgac atcctggccg tcgagctgtt gggcgtcatc
     1681 cccgaagacg agcgcatcct cgtggccagc aaccagggct tgccgctggt gatgcatggg
     1741 ctgaacggca gcagcgccgg cgaggccttc cgccacgtgg cccgccgcct ggacggcgaa
     1801 agcgtgccct tcatggacct gagcgtcaaa ccgaccttca tgagccgcct gcgcgccttc
     1861 atgaacggcc agaattgaga aggagcaacg catgagctgg ctcaatcgat tgctcggtaa
     1921 aaacgaaacc agcggccaga tcgccaagca gcgcttgcag atggtgttga cccacgatcg
     1981 cgccgacctg ccgcccggtc tgttagagca gatcaaggac gacatcatcg aggtcatcgc
     2041 ccgacacacg gccgtcgata cggtcaatgt cgccgtgcac ttggagcaga acgcgcgcga
     2101 gaaccggctg atcgccgagg tgcccctgct ggccgcacgg agagggacta ggggataggg
     2161 gctagggacg aggttccagg ggccaggttc cagggaagaa cgctcttccc tggaacctgg
     2221 aacctggccc ctatcctgtg aaatcggtga aatccctggt ttcgcccccg gcttgcaggc
     2281 acaataggcg gctatgctca gcaagacctc agtctggcgc tacttcgatg tttggctact
     2341 ggcggcggtg ctgctgctga cggcctatgg catcctgatg atccgtagcg ccatcaccgg
     2401 cgcgcccaat ttcgctggct acccccagca gcaactcatc tgggccatcc tcggtctgat
     2461 catcctgctg gtcgtggccg ccatcgacta ccgcgttctc acatccgcgc actggtatat
     2521 ctatggcggg ttggtcttgc tgctcgtggc cgtcatcgtc accggccagg tcaacaatga
     2581 ggcccggcgc tggattcaga tcacgccgtc gttccagatt cagccgtcgg aattcggccg
     2641 catcttcatc acggtcacct tcgcccaatt cctggccaat cggcggcacc tcatcggccg
     2701 cttctccaac accctgctga ccctggccta tttcgccctg ccggtcggcc tgatcttcat
     2761 ccagccgaat ctgggcatga ctctcttgtt catgttcatc tggttcatca tgctctggct
     2821 ggccgggctg ccgctgcgac acttcgcggc cctgctattt cttggcgttg ggctgggcat
     2881 cgccatattc cccttgctgg ccgactacca gaaagaccgc gtgtccacgt ttctgaaccc
     2941 tgaggcagcc aatgaggacg acgtgtttaa cgtcgatcag gccgagatca gcatcggcag
     3001 cggcggctgg ctgggcaagg gctacttgca gggcacacag agccagcttg gcttcctgcg
     3061 cgcccaacac accgac
//
LOCUS       DAOSMQ010000390         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31850_length_3073_cov_3.667992, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000390 DAOSMQ010000000
VERSION     DAOSMQ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31850_length_3073_cov_3.667992"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..698)
                     /locus_tag="PK829_11715"
     CDS             complement(138..698)
                     /locus_tag="PK829_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU41924.1"
                     /translation="MPKLQTQGVHHITLIGADRQTSIDFWEGVLGMPFIFEQPNLDVP
                     AESHLYFDPGDGRLITIFTREDRQPTGENTPEVIGAVHHLAFNVSRATYTQVVARLAE
                     RGIANSGEVDRGFMYSIYFRDPLGFLIELANYKFEPPAGYSHAEVLLEAHLLRVARGD
                     HHIADGHVADAIEALTQRGTRSLSRL"
     gene            857..2629
                     /locus_tag="PK829_11720"
     CDS             857..2629
                     /locus_tag="PK829_11720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU41925.1"
                     /translation="MEPTILDPGSLFYRVFHDSPVGMVVTTLAEGRYVEVNQAYAQML
                     GYAPAELIGQAFPVFGLGHSEERGMVVEVLRRAGKLGDVPLLLRTREGETRTAIGSVQ
                     LEEIDGASYLVSMIQDLTDHEQVQSALQSAESRFRIFFQSIPLPLLVYDVETLRFVDV
                     NPAACRQYGYSHDEFTALSAADLWVISDDNEWAQVLTRVRDPKETHLGVGRHRRKDGA
                     LIDVDVVSYAFSMEGRPVRLSISQDVTEQRAVEMALRASEERLRVIADVTTDAIWDRD
                     LNTNVVLWNHGLHSLFGFAPEDSASHAWWRDHVHPDDREEVEKTIRAFMTAQEGYWAS
                     EYRFLCADGHYANVLDRGYLICDENNQPVRFIGAMVDITAQIQVAEAAARAAQDERQR
                     LARDLHEAVTQSLYSASLMAEAARRHSATADLSTTGDYINRLSELSQQALRQLRLLVY
                     ELRPTLLEQEGLVGALRHRLEAVEQRAGIQARLVEQGEVAVPPGLQAEMFWIAQEALN
                     NALKHSASTATTVVVGAADDAVWLEVKDNGCGFEVDGSSANGAGLARLRERMVKLGGK
                     LEIESVPGGGAVVRARLPLDGNEV"
     gene            2629..>3073
                     /locus_tag="PK829_11725"
     CDS             2629..>3073
                     /locus_tag="PK829_11725"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU41926.1"
                     /translation="MADSRHNFGHEHASDTHPPSPLPTSDFLLADAPMFARVFHNSPV
                     GMVIAAPDGRYLAINDAFARMIGWSVAALLGHSPVELGLMSAADSAALSGSAPRAPVR
                     HLPPAGAGQSDVALQMIGGDGRPRDLVASSQSIELRGRPYVVNLFQ"
BASE COUNT          523 a         1026 c          971 g          553 t
ORIGIN      
        1 ggactttgtg gcctacggac agggcgtggc cgaagaacta cccggatgaa aggcccgtag
       61 agacgggtcg ctgacccgtc tctagtacag acgggtcggc gacccgtctc tagtacagac
      121 gggtcggcga cccgtctcta caaccgcgac aacgatcgcg ttcccctctg cgtcaacgcc
      181 tcaatcgcgt cggccacgtg gccgtcggcg atgtggtggt cgccgcgggc cacgcgcagc
      241 aggtgggcct ccagcagcac ttcggcatgg ctgtagcccg ccggcggctc gaacttgtag
      301 ttggccagct cgatcaggaa ccctaacgga tcgcgaaagt agatcgaata catgaagccg
      361 cggtcgactt cgccgctgtt ggcgatgccc cgctcggcca gccgcgccac cacctgggtg
      421 taggtggccc gcgagacgtt aaaggccagg tggtgaaccg cgccgatgac ttcgggcgtg
      481 ttctcgccgg tcggctgccg gtcttcgcgg gtgaagatcg tgatcagacg gccatcgccg
      541 gggtcgaaat agagatgact ttcggccggc acatccaggt tcggttgctc aaaaatgaac
      601 ggcatcccca ataccccttc ccagaagtcg atcgacgtct gccggtcagc cccgatgaga
      661 gtgatatggt ggacgccctg cgtctgtagt tttggcatca tcgctccttc cttgtgtttg
      721 ctggccgggt tatcccagcc tgtcgggcga ttatagccac tgcccctgtc tgtcagaaac
      781 ccgacatttg acccatgatt gcctctggct acctctatac aatacgtata cacatttctg
      841 tctgtgcgcc gcgactatgg agcctacaat acttgatccg ggttcgcttt tctatcgcgt
      901 cttccacgat agtcctgtgg gcatggttgt cactaccctg gcggaggggc gctacgtgga
      961 ggtcaaccag gcttacgccc agatgttggg gtacgcgccg gccgagttga tcggccaggc
     1021 ctttcccgtc tttggcctgg ggcatagcga agagcgcggc atggtcgtcg aggtgctgcg
     1081 tcgcgccggc aagctgggcg acgttcctct gctgctgcgc acccgcgagg gcgagacgcg
     1141 cacggccatt ggctcagtcc agttggaaga gatcgacggc gccagctacc tggtgtcgat
     1201 gatccaggac ctgaccgacc acgagcaggt tcagtctgcc ttgcagagcg ccgaaagccg
     1261 cttccgcatt ttcttccaga gcattcccct gccgctgctg gtctacgacg tcgagacgct
     1321 gcggtttgtc gacgtcaacc cggcggcctg ccgccaatac ggctatagcc acgacgagtt
     1381 caccgcccta tcggccgctg acctctgggt gatcagcgac gacaacgagt gggcgcaggt
     1441 tctgacccgc gtgcgcgacc ccaaagagac gcacctgggc gtcggccgcc accggcgcaa
     1501 ggacggggcg ctgatcgatg tcgatgtcgt cagctatgcc tttagcatgg agggccgccc
     1561 cgtgcgcctg tccatctccc aggatgtcac cgagcagcgc gccgtcgaga tggctttgcg
     1621 ggccagcgag gagcggctgc gcgtcattgc cgacgtgacc accgacgcca tctgggaccg
     1681 cgacctgaat accaatgtgg ttctgtggaa ccacggcctg cactccctct tcggctttgc
     1741 gccggaagac agcgccagcc acgcgtggtg gcgcgaccac gtccaccccg acgaccgcga
     1801 ggaggtcgag aagacaatca gggcctttat gaccgcccag gagggctatt gggccagcga
     1861 gtatcgcttt ctgtgcgccg acggccacta tgccaacgtc ctcgaccgcg gctatctcat
     1921 ctgcgacgag aacaaccaac ccgttcgctt catcggcgcg atggtcgata tcaccgcgca
     1981 aatccaggtg gccgaagccg ccgcgcgcgc ggcccaggac gaacgccagc gcctggcccg
     2041 cgatctgcat gaggccgtca cgcagtcact ctacagcgcc agtctcatgg ccgaggccgc
     2101 ccgccgccac tcagccacgg ccgacctgag cacgaccggc gactacatca accggctcag
     2161 cgaactgtcg caacaagcct tgcgccaact gcggctgctg gtctatgaac tgcggccgac
     2221 gctgctggaa caagaggggc ttgtcggcgc tctgcgccac cggctggagg ccgttgaaca
     2281 gcgcgccggc attcaggccc gcctggttga acagggcgag gtcgccgtcc cgcccggctt
     2341 gcaagcggag atgttctgga ttgctcagga agcgctcaac aacgccctga agcactccgc
     2401 gtccacagcc acgaccgtcg tggtcggcgc ggccgatgac gctgtctggt tggaagtcaa
     2461 ggacaacggc tgcggtttcg aggtcgatgg ctcctcggcc aacggcgcgg gcctggcccg
     2521 cctgcgcgag cgcatggtga agttgggcgg caaactggag atagagagcg tgccgggggg
     2581 gggcgcagtg gtccgcgccc ggttgccgct agacggcaat gaggtgtaat ggctgacagc
     2641 cgtcacaact tcggccacga acacgcatct gatacccacc ccccgtcccc cctgccaaca
     2701 tccgacttcc tcctggccga cgcgcccatg ttcgcgcgcg tcttccacaa cagccccgtc
     2761 ggcatggtca tcgccgcgcc agatggccgc tacctggcta tcaacgacgc ctttgcgcgc
     2821 atgattggct ggtcggtggc cgccctgctc ggccactcgc cggttgagct ggggctgatg
     2881 agcgccgctg actcggccgc cctgtcgggg agcgctccac gcgcgccggt caggcacctg
     2941 ccgccggcgg gcgcgggcca gtcggatgtc gccctgcaaa tgatcggcgg cgacggccgc
     3001 ccgcgcgacc tcgtggcctc gtcgcagtcc atcgaattgc gcggccgccc ttacgtcgtc
     3061 aacctcttcc agg
//
LOCUS       DAOSMQ010000391         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31926_length_3066_cov_4.465626, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000391 DAOSMQ010000000
VERSION     DAOSMQ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31926_length_3066_cov_4.465626"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1411
                     /locus_tag="PK829_11730"
     CDS             275..1411
                     /locus_tag="PK829_11730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU41927.1"
                     /translation="MNLALIHDWLNQLGGAEDVLAALVSLYPDAPLYTSLYDRARMPA
                     HWREWDIRTAFIDRLPFAHRKQQLYFPLYPFAFEQFDLRGYDVVLSNKSGFCHGVITG
                     PETMHVCYCLTPTRYVWRYHQYAEQEDLGRLTRAGLAPFLTFLRQYDRLAADRVDYFI
                     AISDEVRRRIAKVYRREATIIYPPVDVGRFAPSNRVDDYYLFVGRLVPYRRLDVLIEA
                     FNKMGRPLKIAGSGRDRERLEALAGPTVEFLGFVPDADLPDLLARCRAFVWPGEEDFG
                     ISPLQASASGRPVIAYAAGGARETVVPGVSGALFAEQSVAAIIEAVESFDPLVVSPAA
                     IRRHAEQYDITVFKRRIADFVAQKYEEWNSVTPGQSSAATGSSR"
     gene            1357..2097
                     /locus_tag="PK829_11735"
     CDS             1357..2097
                     /locus_tag="PK829_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41928.1"
                     /translation="MELRHTWTILRRYWLLALIPAVLVLVAGLATWRPAPTVYNAGVR
                     FLVGQPPGAAAAEFDEQRYYNWLSSEYIVNGLTDWIRGGRFAAAVSQRLAGQGIDAPA
                     GLIQGGLAVDNARSMLTVSLTAGDPALLQQMLGGVVTVLTEDNATALPQLAGERAVLI
                     QLDEPVVNTIAPGLRSQLDLPLRVALALAAGLGLALLAHYIDPTVRDRAELERLGVPV
                     LGAIPRERKKGSRFQVPGAREEHSSPGP"
     gene            2137..2787
                     /locus_tag="PK829_11740"
     CDS             2137..2787
                     /locus_tag="PK829_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOU41929.1"
                     /translation="MELSDIFRILRQRGWLILVLMALTAAAAFGFSRIQTPVYQSNLK
                     LLVLPSRTDFGQAQAAKTLLSSYEQWLRSTYRAQAVINDLQLDMTAGDLLGDVRVASD
                     ANAFVIDLTVENTDPNLANDIARSWGNQLIQWQNANNDKLQKEDRITIEFLDDPQAGL
                     DRPDTKINTAAGAVFGLLLGVALLFLLEWLASGVMRRSEDVERYLNIAVIGTIPQE"
     gene            2834..>3066
                     /locus_tag="PK829_11745"
     CDS             2834..>3066
                     /locus_tag="PK829_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOU41930.1"
                     /translation="MSLVTITDPRSPAAEAYRTLRTNLSFYSLDQPIHTLVVTSPAAG
                     EGKSTAIANLAVTMAQSGRRTILVDCDLRRPSL"
BASE COUNT          487 a         1064 c          977 g          538 t
ORIGIN      
        1 ggcgaggtct ggcgcagaaa gtagttggca aggagcaagg cggccaggcc gaaagcgtag
       61 agcagcccgg cggcgtggat ggccgcgcgt agacggttgt ggttggcagg cttgaccatc
      121 ttgaccatgt tcaagatcga ttatagtggc gagcacgcgc tttgccgatc aaattgcggc
      181 ttggcatgag gtgtcttaat gcctttgtcg tgaccccgca ccatcaaccg cgaatgctgc
      241 cgttgcggcc gtttcccggc tataatccct ctctgtgaac ctcgccctca tccacgactg
      301 gctcaaccaa ctcggcggcg cggaggatgt gctggcggcg ctggtgtcgc tctatcccga
      361 cgcgccgctg tatacctcgc tctacgaccg cgcgcgcatg ccagcccact ggcgcgagtg
      421 ggacattcgc acggccttca tcgaccggct gcccttcgcc caccgcaagc agcagctcta
      481 cttcccgctc tacccgtttg ccttcgagca gttcgacctg cgcggctacg acgtggttct
      541 cagcaacaag agcggctttt gccacggcgt catcaccggg ccggagacga tgcacgtctg
      601 ctactgcctg acgccgacgc gctacgtctg gcgctaccac cagtacgccg agcaggaaga
      661 cctcggccgc ctgacgcgcg ccggcctggc cccgttcctg accttcctgc gccagtacga
      721 ccgcctggcg gccgaccgcg tggactattt catcgccatt tcggatgaag ttcgtcggcg
      781 aatcgccaag gtttaccggc gcgaggcgac tattatctac ccaccggtgg acgtggggcg
      841 cttcgcgccg tcgaatcggg tggacgacta ctacctgttc gtcggccgcc tcgttcccta
      901 ccggcggctc gatgtgctca tcgaggcgtt taacaagatg ggccggccgc tcaagatcgc
      961 cggcagcggc cgcgaccggg agcggctgga agcgttggcc gggccgacgg tcgagtttct
     1021 gggcttcgtg cccgacgccg acctgcccga cctgctggcc cgctgccggg cgtttgtctg
     1081 gccgggcgaa gaggacttcg gcatctcgcc cttgcaggcc agcgcctccg gccggccggt
     1141 catcgcctac gccgccggcg gcgcgcgcga gacggttgtg cccggcgtca gcggggcgct
     1201 gtttgccgag cagtccgttg ctgctatcat cgaggcggtc gagtcgttcg atccgctcgt
     1261 ggtcagtccc gcggccattc gccgtcacgc cgagcagtac gacatcaccg tgttcaagcg
     1321 ccgcatcgcc gactttgtgg cccaaaagta cgaagaatgg aactccgtca cacctggaca
     1381 atcctccgcc gctactggct cctcgcgctg atcccggctg tgctggtgct cgttgccggg
     1441 ctggcgacgt ggcggcccgc gccgacggtc tacaacgccg gcgtgcgctt tctcgtcggc
     1501 cagccgcccg gcgcggcggc ggccgagttc gatgagcagc gctactacaa ctggctgtcg
     1561 tcggagtaca tcgtcaacgg cctgaccgac tggattcgcg gcgggcgctt tgccgccgcc
     1621 gtcagccagc gcctggccgg gcagggcatc gacgcgccgg ccgggctgat tcagggtggg
     1681 ctggccgtgg acaacgcgcg cagtatgctg accgtctcgc tgacggccgg cgacccggcg
     1741 ctgttgcaac agatgctcgg cggcgtcgtc acggtgctga cggaggacaa cgccacggcc
     1801 ctgccacagc tggccggcga acgggcggtg ctgatccagc tcgatgaacc agttgtcaac
     1861 accatcgccc ccggcctgcg cagccaactc gacctgccgc tgcgcgtggc cctggcgctg
     1921 gcggccgggc tggggctggc gctgctggcc cactacatcg acccgacggt gcgcgaccgc
     1981 gccgaactgg agcgcctggg tgtgcctgtg ttgggcgcga ttccgcggga gcggaagaag
     2041 ggttccaggt tccaggtgcc aggtgccagg gaagagcact cttcccctgg cccctagtcc
     2101 ctggcccctc gccccttctg ataagagacg acacatatgg aactaagcga catttttcgc
     2161 atcttgcgcc agcggggctg gctgatcctg gtcttgatgg ccctgacggc cgcggccgcc
     2221 tttggcttca gccgtatcca gacgcccgtc taccagtcga acctgaagct gttggtgctg
     2281 ccgtcgcgca ccgacttcgg ccaggcgcag gcggccaaga cgctgctgag cagctatgag
     2341 cagtggctgc gcagcaccta ccgcgcccag gcggtcatca acgacctgca actggacatg
     2401 accgccggcg atctgctggg cgacgtgcgc gtggcctccg acgccaacgc cttcgtcatc
     2461 gacctgacgg tggagaacac cgaccccaac ctggccaacg acatcgcccg ctcgtggggc
     2521 aaccaactga tccagtggca gaacgccaac aacgacaagc tgcaaaagga agaccgcatc
     2581 accatcgagt tcctcgacga cccccaggcc ggcctggatc gcccggacac aaagatcaac
     2641 accgccgccg gggccgtctt cgggctgctg ctgggcgtgg ccctgctgtt tctgctggag
     2701 tggctggcct ccggcgtgat gcggcgcagc gaggatgtcg aacgctatct caacatcgcc
     2761 gtcatcggca ccatcccgca ggagtaagca tcgacgtggc ggttcgccgc cgcgtaaggt
     2821 caatcgaagc accatgagcc ttgtgaccat taccgatccc cgttcccccg cggccgaggc
     2881 ctaccgcacg ctgcgcacga acctgtcgtt ttacagcctt gaccagccca tccacaccct
     2941 ggtggttacc tcgcccgcgg ctggcgaggg caaatcgacg gccatcgcca atctggccgt
     3001 gacgatggcc cagagcggcc gccgcaccat cctggtcgat tgcgacctgc gccggccgtc
     3061 gctcca
//
LOCUS       DAOSMQ010000392         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_31945_length_3065_cov_5.464120, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000392 DAOSMQ010000000
VERSION     DAOSMQ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31945_length_3065_cov_5.464120"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1564)
                     /locus_tag="PK829_11750"
     CDS             complement(83..1564)
                     /locus_tag="PK829_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOU41931.1"
                     /translation="MFKKILIANRGEIAVRVIRTCREMGISTVALYEASDRGSLHVRL
                     ADECVALPGPQSFMDPAAILAIAQEQGVDAVHPGYGYLAEDVAFIRTCDEAGIAFIGP
                     PAEVVERLRNKIGALETARAAGIPTVVHSPRSFSENEFEGLAAAAEAIGYPVVLKSCR
                     GGRGRGERVVLTAAQLPDVARRAQVETQAVYGNKQLYVERAILPAHQVGVQVLADHYG
                     NLVHLGEREGSVVQSNQKIVEEGPALCLSEARRAELLATALQVARLFEMQNLGTVEFL
                     VDGNGDFYFSEVKPRIQIAHTLAEMLTRIDLVREQIRLEAGEPLNLRQEDLVRRGHAI
                     MCRLQAEDPFHRYLPAPGHLRQVRMPGGPEVRVDTYVYAGADVPSHYDALIAKITAWA
                     PDRATCIDRMRRALEDANVSGAPTNLPLLLRILRDEAFVRGDYSTDVLSRLEVAPVPE
                     FDRVQRDLAVAAALIYARRREAFNPQTPDQWSSGWHRSSRQLS"
     gene            complement(1635..>3065)
                     /locus_tag="PK829_11755"
     CDS             complement(1635..>3065)
                     /locus_tag="PK829_11755"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOU41932.1"
                     /translation="RIDLLFDPGTFTEIDTLVLPRHETYPGGKGSRLGDGVITGFGLI
                     NGRRAFASSQDAAVMGGSLGEMHANKIVKAMQMALKYGCPFIAINDSGGARIQEGVDS
                     LGGYARIFDANCEASGVIPQLSVIMGPCAGGAVYSPALTDFVFMTNNSYMFITGPDVV
                     RSVMQENVSFDELGGGMMHSQESGVCHFLTTDDRDCLARVRTLLSYLPGNNQDDPPYV
                     PPIDDPERRCPELNELAPTDPYHAYDVRTVIGSIVDDGAFFEVHQLWAENMVVGFARL
                     NGYVVGIVANQPMVLAGCIDIKASIKATHFIRICDAYNVPIITLQDVPGFLPGRDQEY
                     GGIIRNGARMIYAYSEATVPKLLVILRKSYGGAYCVMSSRGLRGDLLYAWPNAEIAVM
                     GAEGAVNILFRRDVQAAEDPEARRRELVDDYQRKFNNPYVAAARGLIDDVIDPADTRR
                     VLIRSLQMTMSKRERHIPRKHGISPM"
BASE COUNT          507 a          977 c         1054 g          527 t
ORIGIN      
        1 gctgaccaga cccagctcga cctcgtaggc cgtgccgtca attgtcacca gtagggacgt
       61 catcaatcct cgctccttgt cgttagctca attggcggct gctgcggtgc cagccactcg
      121 accactggtc gggcgtctgc gggttgaagg cctcgcggcg gcgggcgtag atgagggccg
      181 cggccacggc caggtcgcgc tgcacgcggt cgaactccgg cacaggcgcg acttccagac
      241 ggctgagcac gtcggtggag tagtcgccgc gcacgaaggc ctcgtcgcgc aagatgcgca
      301 ggagcagcgg caggttcgtc ggcgcgccgc taacgttggc gtcctccagc gcgcgccgca
      361 tgcggtcgat gcaggtggcg cgatccggcg cccaggcggt gatcttggcg atcagggcgt
      421 cgtagtgaga tggcacgtcg gccccggcat agacataggt atcgacgcgc acttccggcc
      481 cgcccggcat gcgcacctga cgcaggtggc cgggcgcggg caggtagcgg tggaaggggt
      541 cttcggcctg caggcggcac atgatggcat ggccgcgccg gacgaggtct tcctggcgca
      601 gattcagcgg ctcgcccgcc tccagccgga tctgctcgcg caccaggtcg atgcgggtca
      661 gcatctccgc cagtgtgtgg gcgatctgga tgcgcggctt gacctcgctg aagtagaagt
      721 cgccgttgcc atccaccagg aactcgaccg tacccaggtt ctgcatctcg aacaggcgcg
      781 ccacctgcag ggccgtagcc agcagttctg cccgccgcgc ctccgagagg cacagcgccg
      841 gcccctcttc gacgatcttc tggttgctct gcacgaccga cccctcgcgc tcgcccaggt
      901 ggacgaggtt gccgtagtgg tcggccaaca cctgcacgcc gacctggtgg gcgggcagga
      961 tggcgcgttc gacgtagagc tgcttgttgc cataaacggc ctgcgtctcg acctgcgccc
     1021 ggcgggcgac gtcgggtagc tgcgcggccg tcaggacaac acgctcgcca cgcccacgcc
     1081 cgccgcgaca cgacttgagc accaccggat agccgatagc ctcagccgcc gcggccagcc
     1141 cctcaaattc gttttcggag aacgaccgcg gtgaatggac aaccgtcggg atgccggcgg
     1201 cgcgcgccgt ctccagcgcg ccgatcttgt tgcgcagccg ttcgacgacc tcggccggcg
     1261 ggccgatgaa ggcgatgccc gcttcgtcgc aggtgcggat gaacgccaca tcttcggcca
     1321 ggtagccata gccggggtgg acggcatcga cgccttgctc ctgggcgatg gccagaatgg
     1381 cggccgggtc catgaagctc tgcggccccg gcagggcgac gcattcgtca gccagacgaa
     1441 catggagcga gccgcggtcg ctggcctcgt agagggcgac cgtcgagatg cccatctcgc
     1501 ggcaggtgcg gatgacacgg acggcgatct cgccgcgatt ggcgatgagg attttcttaa
     1561 acatgatcgt tccttttctt cagaggctag gggccaggga gaccatctct tccctagccc
     1621 ctagccccta atccctacat cggcgagatg ccatgtttgc gcggaatgtg gcgctcgcgc
     1681 ttggacatgg tcatctgcaa cgaacggatg agcacgcgac gcgtgtcggc cgggtcgatg
     1741 acatcatcga tcagcccgcg ggcagcggca acgtaggggt tgttgaattt gcgctggtaa
     1801 tcgtctacca gttcgcgccg ccgggcctcc gggtcttcgg ccgcctgcac gtcacggcgg
     1861 aagaggatat tgaccgcgcc ttccgcgccc atgacggcga tctcggcgtt gggccaggcg
     1921 tagagcaggt cgccgcgcag accgcggcta ctcatgacgc agtaggcccc gccatagctc
     1981 ttgcgcaaaa tgaccaataa cttgggcaca gtggcctcgc tgtaggcgta gatcatgcgc
     2041 gcgccgttgc ggatgatgcc gccatactcc tggtcgcggc cgggcaggaa gccgggcacg
     2101 tcttgcagag tgatgatggg cacgttgtaa gcatcgcaga tgcggatgaa gtgcgtggcc
     2161 ttgatcgatg ccttgatgtc gatgcagcca gccagcacca tcggctggtt ggcgacgatg
     2221 ccgacgacat agccgttcaa gcgggcgaag ccgacgacca tgttctcggc ccacaactgg
     2281 tgcacctcga agaacgcgcc atcgtcaacg atgctgccga tgacggtgcg cacgtcgtag
     2341 gcgtggtagg gatcggtcgg cgccagttcg ttcagctccg ggcagcgccg ctcagggtcg
     2401 tcaatgggcg gtacgtaggg cgggtcgtcc tggttgttgc cgggcaggta gctcagcagg
     2461 gtgcgcacgc gggccaggca gtcgcggtcg tcggtcgtca ggaagtggca gacgccgctc
     2521 tcctgactgt gcatcatgcc gccgcccagc tcgtcgaagc tgacgttctc ctgcatcacc
     2581 gagcgcacaa cgtccggccc ggtgatgaac atatagctgt tattggtcat gaagacgaag
     2641 tcggtcaggg ccggcgagta gaccgcgccg ccggcgcacg gccccatgat gaccgacagt
     2701 tgcgggatga cgcccgacgc ctcgcagttg gcgtcgaaga tgcgcgcata gccgccgagg
     2761 ctgtcgacgc cttcctggat gcgcgcgccg ccgctatcgt tgatggcgat gaaggggcag
     2821 ccgtacttga gggccatctg catcgccttg acgatcttgt tggcgtgcat ctcacccagg
     2881 gagccgccca tgacggcggc atcctgcgag gaggcgaagg cgcgccggcc gttgatcagc
     2941 ccgaagccgg tgatgacgcc gtcgcccagg cgcgagcctt tgccgccggg gtaggtctcg
     3001 tggcggggca agacgagcgt gtcgatttcg gtgaatgtgc cggggtcaaa aagcaggtcg
     3061 atgcg
//
LOCUS       DAOSMQ010000393         3054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_32095_length_3054_cov_4.195398, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000393 DAOSMQ010000000
VERSION     DAOSMQ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32095_length_3054_cov_4.195398"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..778)
                     /locus_tag="PK829_11760"
     CDS             complement(56..778)
                     /locus_tag="PK829_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU41933.1"
                     /translation="MDTLLRALIRGFARLLFRLALRLEIEGREHVPPGGPLVIIGNHF
                     SLFEAPLLICFLPYGDSVTWLAATELQENPFLRALIPLFNIIPVWRGQPDRAALRRAF
                     DWLAAGGVVGIMPEGGVDDSLRHLTLAGELTTDANGPQSRLNAQLITPRPGAAYLAVR
                     SGAPVLPTACLGTERILANVRRLRRTPVRLIIGPVFGPLTIDESLPKAERRERLDALG
                     HEMMRHMAALMPPENRGPYRDA"
     gene            complement(876..>3054)
                     /gene="pheT"
                     /locus_tag="PK829_11765"
     CDS             complement(876..>3054)
                     /gene="pheT"
                     /locus_tag="PK829_11765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU41934.1"
                     /translation="GIILVRSDEIRAGEQGSRGAGERERADRGLSPAPLPPRPPALPA
                     PGTPLQDVFGDAVLEIAILPNTARATSIIGVARELAALTGKPLRLPGYDVVAAPASSS
                     RPPVDQRVVITTDRPDLNPRFVALLIEGVQQIASPYWMQHRLRLAGQRPINVVVDISN
                     YVMLEVGQPNHTFDYDVLRRRADGYGPGGSEAGSNPIHIHTRLPHPGETVTTLDGAER
                     KLEPYTILVTDPAGNLSIGGIMGGRESEIGPTTTNVLLEAASWDFINIRRSANALDLH
                     TEAAYRFSRGVPPSLALLGAKRAAELLRVLAGGTVAPGIVDTYPNPAPPVVIDLDPVY
                     ARQLSGLELPAGEIAALLRRLAFVVEPAGEHLRVTVPDHRLDIEGPHDLVEEICRLYG
                     YDRIPSTLLADTLPPQRRNVALEREERLRDLLAQLGLQEVWTYRLTSAAREARLLPVG
                     QPDDRPYVTLSNPPAPDRYAMRHSVLSSVLEVVAANSRHTPRIALFEIGAIYLGSEDG
                     PLPDELPRLAIAMTGRRQVEDWTDGMPASFDFYDLKGVMDALFAALHLPVTVEAAAHP
                     SYRPGRCARLLVGQQVIGTLGELHPGVVKGFDVRVERDQAVLAADIDLQALLPLIPDS
                     YRYTALPAYPPVREDLALVVEASLPAATVEAALRAAGGALLRDVALFDVYDGSQLPPG
                     KKSLAYHLTFQAPDKTLTDADVSRQRGRILRLLEQQLGAMLRS"
BASE COUNT          452 a          991 c         1159 g          452 t
ORIGIN      
        1 gcgagctggg cagagatttg cgttggcgcg gcggccgcgg cgtgcccgta gcgggctagg
       61 cgtcgcggta ggggccgcgg ttctccggcg gcatcagcgc ggccatgtga cgcatcatct
      121 cgtggccgag ggcgtccagc cgctcgcgcc gttcggcctt cggcagtgac tcgtcgatgg
      181 tcagcgggcc aaagacgggg ccgatgatca gccgcaccgg cgtccggcgt aggcggcgga
      241 cgttggccag gatgcgctcc gtgcccagac aggccgttgg cagaacgggc gcgccgctgc
      301 gcacagccag ataggccgcg cccggccgcg gggtgatgag ctgggcgttc aggcgtgatt
      361 ggggaccgtt ggcatcggtg gtcaactcgc cggccagggt cagatggcgc agcgagtcat
      421 cgacgccgcc ctccggcatg atgccgacca cgccgccggc cgccagccag tcgaacgccc
      481 gccgcagagc cgcccgatct ggctggccgc gccagacggg aatgatgttg aacagaggga
      541 tgagcgcccg caaaaagggg ttctcctgta gctccgtcgc cgccagccag gtaacgctgt
      601 cgccataggg caggaagcaa atcagcaacg gcgcttcaaa gaggctgaag tggttgccga
      661 tgatcaccag cggcccgccg ggcggcacat gctcgcgccc ctcaatctct aaccgcaggg
      721 ccaggcgaaa gagcaaccgc gcgaagccac gaatgagggc gcgaaggagg gtgtccatat
      781 agggtcgctt gtgccggggg gctggggggc aggggagcag gggagcaggg gggagcaccc
      841 ccgctccccc gcccccctgc tcccctgcgc ttccactagc tgcggagcat ggcccccagc
      901 tgctgctcca gcagccgcag gatgcgcccg cgctggcggg agacgtcggc gtcggtcagc
      961 gtcttgtctg gagcctggaa ggtcaggtga taggccaggc tctttttccc cggtggcaac
     1021 tggctgccgt cgtagacgtc gaacagggcc acgtcgcgca gtagcgcccc gccggccgcc
     1081 cgcagtgccg cctcaaccgt ggccgcgggt agtgacgcct cgacgacgag ggccaggtct
     1141 tcgcgcacgg gcgggtaggc cggcagcgct gtgtagcggt agctgtcggg gatgagcggc
     1201 agcagcgctt gcaggtcgat atcggcggcc agcacggcct ggtcgcgctc gacgcgcacg
     1261 tcgaagcctt tgaccacgcc ggggtgcagc tcgcccagcg tgccgatcac ttgctgacca
     1321 acgagcagac gggcgcagcg gccgggccgg tagctggggt gggctgccgc ctcgacggtg
     1381 acgggcaggt gcagcgcggc aaaaagcgca tccatgacgc ccttcaggtc gtagaagtcg
     1441 aacgaggccg gcatgccgtc agtccaatcc tccacctgtc gccggccggt catggcgatg
     1501 gccagccgcg gcagttcgtc gggcagtggc ccatcctcgc tgcccaggta aatcgcgccg
     1561 atctcgaaca gggcgatgcg cggcgtgtgg cggctgttgg ccgcgactac ctccagcacc
     1621 gacgacagga cactatggcg catggcgtag cggtcgggcg cgggcgggtt gctcagggtg
     1681 acgtagggcc ggtcgtcggg ctggcccacg ggcagcaggc gcgcctcgcg ggcggccgaa
     1741 gtcagccggt aggtccagac ctcctgcaag cccagctgcg ccagcaggtc gcgcagccgc
     1801 tcctcgcgct ccagggccac gttgcgccgc tgtggcggca gtgtgtcggc cagcagcgtc
     1861 gaggggatgc ggtcgtagcc gtagaggcga caaatctcct cgacgaggtc gtgcggcccc
     1921 tcgatgtcca gccgatggtc gggcacggtg acgcgcagat gctcgccggc cggctcgacg
     1981 acgaacgcca gccgccgcag cagggcggcg atctctccgg ccggcagttc cagcccgctg
     2041 agctggcgcg cgtagaccgg gtcgaggtcg atgacgacgg gcggggcggg gttggggtaa
     2101 gtatcgacga tgcccggcgc gaccgtgccg ccggccaaga cgcgcagcag ctcggcggcc
     2161 cgcttggctc ccagcagggc cagactgggc ggcacgccgc ggctgaagcg gtaggcggct
     2221 tcggtgtgca aatccagcgc gttggcgctg cggcggatgt tgatgaaatc ccacgacgcc
     2281 gcttcgagaa ggacgttggt cgtcgtcggg ccgatctcgc tctcgcggcc gcccatgatg
     2341 ccgccaatgg acaggttacc ggccgggtcg gtgacgagga tggtgtacgg ctccagcttg
     2401 cgctccgcgc catccagcgt cgtgacggtt tcgccggggt ggggcaggcg agtgtggatg
     2461 tggatcgggt tggaacccgc ttccgagccg ccggggccat agccatcggc gcggcggcgc
     2521 agcacgtcgt agtcgaaggt gtggttgggc tggccgacct cgagcatgac gtagttgctg
     2581 atatcgacga cgacgttgat gggccgctgg ccggccagcc gcaggcggtg ctgcatccag
     2641 taggggctgg cgatctgctg cacgccctcg atgagcaggg cgacaaagcg cgggttcagg
     2701 tcgggccggt cggtggtgat gacgacgcgc tggtcgacgg gcgggcggct gctgctggcc
     2761 ggggcggcga ccacgtcgta gccaggcagg cgcagcggct tgcccgtcag cgccgccagt
     2821 tcgcgggcca cgccgatgat cgacgtggcc cgggccgtgt tgggcaggat ggcgatctcc
     2881 agcacggcgt cgccgaacac gtcttgcagc ggcgtgccgg gagctggcaa tgcagggggg
     2941 cgggggggca ggggcgcggg ggagaggccg cggtcggctc tttctctttc ccctgctccc
     3001 ctgctcccct gctcccctgc tcttatttca tcactccgca ccaggatgat gccc
//
LOCUS       DAOSMQ010000394         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_32175_length_3049_cov_4.479292, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000394 DAOSMQ010000000
VERSION     DAOSMQ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32175_length_3049_cov_4.479292"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK829_11770"
     CDS             <1..293
                     /locus_tag="PK829_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41935.1"
                     /translation="ADFTFFAQLVGPDTTRYAAIDTPPPTPTGQWTPGEVYEVRLPLA
                     LDPATPPDAYPLIIGLYTRTADGGFDRLQQVTPDGRLTDDFMTLTLVRVDAR"
     gene            404..1861
                     /locus_tag="PK829_11775"
     CDS             404..1861
                     /locus_tag="PK829_11775"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU41936.1"
                     /translation="MRLGFFAPWRERSSPNPYPLSPSPLLLGLLVGLIAVLAGVVLGF
                     GGPVAAAAVALGGLAAVLVLRNMELGFFAVIGVVALLPFATLPFDIGLTPTFLDFALG
                     AAVLVWLMGIVTGRQRDLVTAPVLVPLLLFIIVAVFAFIFGLGNGPLTPTLLRKFAEL
                     LISLGFVLVVIDYCRTWPRLERLVKFLLLMGGAAAVIAIGLWLLPDSTANSALNALTR
                     LGYPGGWVIRYIEENPELSERAIGTSVDPNSLGGLLLMIGALIGPQVVTRQPLFRRPL
                     VLLIAGLVIVALILTFSRGAMLGLAAGLGFVALTRYRRLLPWLALAALLLLVLPITQG
                     YIARFVEGFQGADLATQMRFGEYKDALILIGRYPIFGVGFAGTPDIDVYLAVASVYFT
                     IAGRMGLLGLLGFFTIMAVVFGYAHRHRHALRADERRDAVWLGLHAALVGALVAGVFD
                     HYLFNMDFPHAVTIFWMVLGLAVAGTRLGATTETR"
     gene            2037..>3049
                     /locus_tag="PK829_11780"
     CDS             2037..>3049
                     /locus_tag="PK829_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU41937.1"
                     /translation="MTAHRLGRALVALAVLALLLALWPNARSLADLTGEEALRGQAAG
                     LVHWLNAAIRPQPRLEPTAVAGSPLVYPAVSPFAVNTFLEQEAEPEKRARILDLAAAA
                     GFRAIRQEFPWEDIEVHAKGDFIDRRNDSAGVDAWAKYDQIVALAGARDIAVIARLSN
                     PPSWSRARGDELGTNAPPDDFNDFGDFATAVAERYRGRIHFYQVWNEPNGNEEWGLQD
                     VDPEAFTRLLCLAYDRIKAVDPSAVVLAGALTPTVANDGGNMNDLLFLERMYAAGAGG
                     CFDVMSAQGYGLWSGPTDQRLRPTVINYPHNLFLRDLMVRHGDAAKPIWISEMGWNVA
                     PEG"
BASE COUNT          419 a         1078 c          970 g          582 t
ORIGIN      
        1 cggccgactt caccttcttc gcccagctcg tcggcccgga cacgactcgc tacgcggcca
       61 tcgacactcc accgccaacg cccaccggcc agtggacgcc cggcgaggtc tacgaggtgc
      121 gcctgccgct ggccctcgac ccggccacgc cgcctgacgc ctatccgctc atcatcggcc
      181 tgtacacgcg cacggccgac ggcggcttcg accgcctcca gcaggtgacg cccgatggcc
      241 gcctgaccga cgacttcatg actttgacgc tcgtgcgggt ggatgcgcga tgagcagaca
      301 ggggctaggg gctaggggcc aggggctagg ggaagagact cacgccaagg cgctaagggg
      361 ccaagccgca aaggaagaag agcaagattc ttcttctctg cttttgcgcc ttggcttctt
      421 tgcgccttgg cgtgagcgtt cttcccctaa tccctatccc ctatccccta gcccccttct
      481 tctcggcctg ctcgtcggcc tcatcgccgt gctggccggc gtcgtgctgg gctttggcgg
      541 gccggtcgcc gcggcggctg tggcgttggg tgggctggcg gccgtgctgg tgctgcggaa
      601 catggaactg ggcttcttcg ccgtcatcgg tgtcgtcgcc ctgttgcctt tcgctacgct
      661 gcctttcgac attggcctga cgccgacctt ccttgacttc gccctgggcg cggccgtgct
      721 tgtctggctg atgggcatcg tcaccggccg gcagcgcgat ctggtgaccg cgccggtgct
      781 tgtgcccctg ctgctgttca tcatcgtcgc cgtcttcgcc ttcatctttg gcctgggcaa
      841 cggcccgctg acgccgacgc tgctgcgcaa gttcgccgaa ttgctcatca gcctcggctt
      901 cgtcctcgtc gtcatcgact attgccgcac ctggccgcgg ctggagcggc tggtcaagtt
      961 cctgctgctc atgggcgggg cggcggccgt gatcgccatc ggcctgtggc tgctgcccga
     1021 cagcacggcc aacagcgccc tcaacgcctt gacccgcctg ggctaccccg gcggctgggt
     1081 catccgctat attgaggaga acccggaact gagcgaacga gccattggca catcggtcga
     1141 tcccaactcg ctcggcggcc tgctgctgat gatcggggcg ctgattgggc cgcaggtggt
     1201 cacgcgccag ccgctcttcc gccggccgct ggtgctgctg atcgccgggc tggtcatcgt
     1261 cgccctcatc ctgaccttct cgcgcggggc aatgctgggg ctggccgcgg ggctgggctt
     1321 tgtggccctg acgcgctacc ggcggctgct gccgtggctg gcgttggcgg cgctgttgct
     1381 gctcgttctg cccatcaccc agggctacat cgcccgcttc gtcgagggct tccagggagc
     1441 cgacctggcc acacaaatgc gttttggcga gtacaaggac gcgctcatcc tgatcggccg
     1501 ctaccccatc ttcggcgtcg gcttcgccgg cacgcccgac atcgacgtct atctggccgt
     1561 ggccagcgtc tacttcacca ttgccgggcg catgggcctg ctggggctgc tgggcttctt
     1621 taccatcatg gccgtcgtct tcggctatgc ccatcgccac cgccatgccc tgcgcgccga
     1681 cgagcgccgc gatgccgtct ggctggggct gcacgctgcc ctggttgggg cgctggtggc
     1741 cggcgtgttc gaccactatc tgttcaacat ggactttccc cacgcggtga cgatcttttg
     1801 gatggtattg gggctggcgg tggcggggac gcgactggga gctactactg aaacccggtg
     1861 aagtgtgggc taagggggct gtccgcgatc agcttcttcg tctcagggct gttccaaagt
     1921 caccccaggc gcgcccaccg gtcaggtcaa tggtcgcgag acgactttgg caaagccctg
     1981 ttcttcgtct caaacagtaa tgcgtgtgcc gcgattgtca ctataatatg ggccacatga
     2041 ccgcccaccg cctcggccgc gccctggtcg ccctggccgt cctcgctctg ctgcttgcgc
     2101 tctggcccaa tgcccgttcc ctggctgacc tgaccggtga agaggccctg cgcggccagg
     2161 cagccggctt ggttcactgg ctcaacgccg ccattcgccc ccagccgcga ttggaaccga
     2221 ccgccgtggc cggctccccc ctggtctacc cggccgtcag ccccttcgcc gtcaacacct
     2281 ttctggaaca ggaggccgag ccggaaaagc gcgcccgcat cctcgacctg gccgccgcgg
     2341 ccggcttccg cgccatccgg caggagttcc cctgggagga catcgaagtc catgccaaag
     2401 gggatttcat cgaccgccgc aacgactccg ccggcgtcga tgcctgggcc aagtacgacc
     2461 agatcgtggc cctggccggg gcccgcgaca tagccgtcat cgcccggctg agcaatccgc
     2521 cgtcgtggag ccgcgcccgt ggcgacgagt tgggcaccaa cgcgccgccc gatgacttta
     2581 acgatttcgg cgatttcgcc accgccgtgg ccgagcgcta ccgcggccgc atccacttct
     2641 accaggtctg gaacgagcca aacggcaatg aggagtgggg cctgcaagac gtggacccgg
     2701 aggcgttcac gcgactgctc tgcctggcct acgaccgcat caaggccgtc gatccgtcgg
     2761 ccgtggtcct ggccggggcg ctgacgccga ccgtggccaa cgacggcggc aacatgaacg
     2821 acctcctctt cctggagcgc atgtacgccg ccggggcagg aggctgcttc gacgtgatgt
     2881 cggcccaggg ctatggcctc tggtctggcc cgaccgacca gcgcctgcgg ccgacggtca
     2941 tcaactatcc ccacaacctc ttcctgcgcg acctgatggt gcgccacggc gacgccgcca
     3001 agcccatctg gatcagcgag atgggctgga acgtcgcccc ggagggcat
//
LOCUS       DAOSMQ010000395         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_32435_length_3031_cov_4.823253, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000395 DAOSMQ010000000
VERSION     DAOSMQ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32435_length_3031_cov_4.823253"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PK829_11785"
     CDS             <1..1181
                     /locus_tag="PK829_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41938.1"
                     /translation="DNAATAMPSTDAVFVPMIGQGVGQMAEAPAPTPMPEPPTATAEP
                     PTATPEPPTTTPAPAALIGASPTPIRVVDFNAVAAELAARDEVLVYNKIGFHVTFLEE
                     SDQLDGWMQRLDAEGVPFFLKSVDNAEPLYKAQELMKVSGVPHTLVYRSTLYDVPDYN
                     LDPVAAAESYWELEKAIWPPELDPSLVWLETMNELDKTRSEWLAQFSLRTAQLALRDG
                     YRWAAFGWASGEPEPDDWESPAMLELLRLIADHPDQLAIALHEYSYTSEDIADDYPHK
                     VGRFLDLFAAADRHGIPRPTVLITEWGWSYDHIPPEPLAMEDVTWAARLYAAYPQIKG
                     AAIWNLGKLEDTLLPLNTQMSALIDNMLALSLDTTFSAPAPPAQTPLDAGLFPPPARD
                     "
     gene            complement(1254..1982)
                     /locus_tag="PK829_11790"
     CDS             complement(1254..1982)
                     /locus_tag="PK829_11790"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOU41939.1"
                     /translation="MTLTLDVLHAELRTCRRCLEEGYWIAPGPVFSSGPEPRVMLIGQ
                     APGPTEAVVKRPFNAGSGKRLFKWLAEAGWDETDFRATAYMTAITKCYPGRSDSGKGD
                     RVATPFEQMLCRPWLQQELRLVNPRLLILVGGLAIRLLYPANTRLDTIIGTAAWFPPA
                     ALAADPWNLDLSQAEVIGAMDGGSAADGRDLSSVVRRHVGGRFVVPLPHPSGASLWPN
                     KPENQALIRRAMALLGDIRAACGL"
     gene            complement(2072..2485)
                     /locus_tag="PK829_11795"
     CDS             complement(2072..2485)
                     /locus_tag="PK829_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU41940.1"
                     /translation="MGFKFERLEVWQLALDYTERIYAVSARLPRDEEYNLKSQIRRAA
                     TSIVLNIAEGSTSQSDAEQRRFVGIAIRSLVETVACMHLINRQYKPADSQPLREAYRD
                     SERLFAKLQAFQHALSDSQIREDKADYEVDALPPF"
     gene            complement(2526..>3031)
                     /locus_tag="PK829_11800"
     CDS             complement(2526..>3031)
                     /locus_tag="PK829_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOU41941.1"
                     /translation="DRKTRAELMDEGLDILTGLWRGQPFSYDGQHYHVRPTEFMPPAP
                     PVQQPRIPIWVVGAWPRPKSLARALRYDGLLPNPMTPEGDHRPLTIDDVRDIAAYVRD
                     HRAEATPFDIVAEGKTPGDRPDEAAATVRDWADAGATWWIEALWGAEDIMAVRQRVIA
                     GPPTADR"
BASE COUNT          519 a         1044 c          967 g          501 t
ORIGIN      
        1 ccgacaacgc ggccacggcc atgccgtcca ccgacgccgt ctttgtgccc atgatcggcc
       61 agggcgttgg gcagatggcc gaggccccag cgccgacgcc gatgcctgag ccgcctacgg
      121 ccacggccga accgcccacg gccacgccgg agccgcccac gaccacgccc gccccggccg
      181 ccctcatcgg cgcgtcgccc acgcccatcc gcgtggtcga tttcaatgcc gtcgccgccg
      241 aactggccgc gcgcgacgaa gtgctggtct ataacaagat cggcttccac gtcacctttc
      301 tggaggagag cgatcagctt gatggttgga tgcaacggct ggacgctgag ggcgtgcctt
      361 tcttcctcaa gagcgtcgat aacgccgagc cgctctacaa ggcacaggag ttgatgaagg
      421 tcagcggcgt gccccacacg ctcgtctatc gctccacgct ttacgacgtg cccgactaca
      481 acctcgatcc ggtcgccgcg gccgagtctt attgggaact ggagaaggcc atctggccgc
      541 cggaattgga cccatcgctg gtctggctgg aaacgatgaa cgagctggac aagacgcgct
      601 ccgagtggct ggcccagttc tcgctgcgca cggcccagtt ggccctgcgc gacggctacc
      661 gctgggcggc tttcggttgg gcgtcgggcg agccggagcc ggacgattgg gagtcgccgg
      721 cgatgctgga gctgctgcgc ctcatcgccg accaccccga ccagttggcc atcgctctgc
      781 acgagtatag ctacaccagc gaggatattg ccgacgacta cccgcacaag gtcggccgct
      841 tcctagatct cttcgccgcc gccgaccggc acggcatccc ccggccgacg gtgctcatca
      901 ccgaatgggg ctggagctac gaccacatcc ccccggagcc gctggcgatg gaagatgtga
      961 cctgggcggc gcggctctac gccgcctacc cgcagatcaa gggcgcggct atctggaatc
     1021 tgggcaagct ggaggacacg ctcctgccgc ttaacacgca gatgagcgcc ctgatcgaca
     1081 acatgctcgc cctcagcctc gacacgacct tcagcgcccc cgccccgccg gcgcagacgc
     1141 ccctcgacgc cggcctcttc ccgccgccgg caagagatta actcaaggcc cagtcgcctg
     1201 tggcgcgatt ctccgaatcg cgtgctgcga ttcggagaat cgcgccacaa ggactatagc
     1261 ccgcaggcgg cgcgaatgtc gcccaacagg gccatcgccc ggcggatgag ggcctgattc
     1321 tccggcttgt tgggccatag actggccccg gagggatgcg gcagggggac gacgaaccgg
     1381 ccgccaacgt gacgacggac gaccgacgac agatcgcggc catccgccgc gctgccaccg
     1441 tccatcgcgc cgatcacctc cgcctgactc aggtcgaggt tccacgggtc ggccgccagc
     1501 gccgccggcg ggaaccacgc tgccgtgccg atgatcgtgt ccagccgcgt gttggccggg
     1561 tagagcagcc ggatagccag cccgccgaca aggatgagca gccgcgggtt cacgaggcgt
     1621 agctcctgtt gcagccacgg ccggcagagc atctgctcga acggtgtggc cacgcggtcg
     1681 cccttgccgc tgtcgctgcg gccggggtag catttggtga tggcggtcat gtaggccgtg
     1741 gcgcggaagt cagtctcgtc ccagcccgcc tcggccagcc acttgaagag gcgcttgccg
     1801 ctaccggcgt tgaacggccg cttgacgacc gcctcggtcg ggccgggcgc ctggccaatc
     1861 agcatcactc gcggctccgg gccgctgcta aacaccggtc ccggcgcgat ccagtagcct
     1921 tcctccagac agcggcggca ggtgcggagt tcggcgtgga gaacgtcgag tgtaagtgtc
     1981 atactgtcat actgtagggc gaccgccgac ggccgaccga cgaccgccgc caaaaggaag
     2041 cgcacgacta cagccgctgg ccgagaagaa gctagaaggg tggaagggcg tcaacctcat
     2101 agtcagcctt gtcttcgcgt atctgcgaat ccgaaagagc atgctgaaag gcctgtaact
     2161 tggcaaacag tctctcgctg tcgcgataag cctcacgaag aggctggcta tccgcgggct
     2221 tgtactgtcg attgatcaga tgcatgcagg cgactgtttc caccagcgac cgaatcgcta
     2281 tgccaacaaa tcggcgttgc tcggcgtcag attgggacgt tgaaccctcg gcgatattaa
     2341 ggacgatgct ggtcgcggcg cgcctaatct gactctttag attgtattcc tcgtcgcgtg
     2401 gtaagcgcgc tgaaactgca taaatgcgct cggtgtaatc tagcgccagt tgccacacct
     2461 caagacgctc aaacttgaac cccattcaac acctcaggct gatagacctc tgtttctggc
     2521 ggcggtcagc ggtcggccgt cggcggtcct gcaatgaccc tctgtcgcac tgccattatg
     2581 tcctccgccc cccacaacgc ctcaatccac cacgtcgccc cagcgtccgc ccagtcgcgg
     2641 acggtggccg cggcctcgtc gggccggtcg ccgggcgtct tgccctcggc cacgatgtcg
     2701 aacggcgtcg cctccgcccg gtggtctcga acgtaggcgg cgatgtcgcg cacgtcgtcg
     2761 atggtcagcg gccggtggtc gccctccggc gtcatcgggt tgggcagcag gccgtcataa
     2821 cgcagggcgc gggccaacga cttcggccgc ggccacgcgc cgacgaccca gatcggaatg
     2881 cgcggctgct gcaccggcgg cgcgggcggc atgaactccg tcggccggac gtggtagtgt
     2941 tggccgtcgt agctgaacgg ctggccgcgc cacaggccgg tgaggatgtc cagcccctcg
     3001 tccatcaatt cggcgcgcgt cttgcggtcg g
//
LOCUS       DAOSMQ010000396         3027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_32493_length_3027_cov_3.848250, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000396 DAOSMQ010000000
VERSION     DAOSMQ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32493_length_3027_cov_3.848250"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1371
                     /locus_tag="PK829_11805"
     CDS             148..1371
                     /locus_tag="PK829_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41942.1"
                     /translation="MKTKIPRNIWVMTAASFLTDVSSEMVNNLVPLFLANVLGARTGT
                     IGLVEGVVESMSSFIKLLSGWWSDRVVSRKPPTVIGYALSAVSKPLLAFAASWTAVLG
                     ARFGDRMGKGIRTAPRDALLADSVPAEGRGAAFGFHRAGDSLGAVGGLALALLAVWLI
                     QGNALSLSRATFQRIALFSAVPALLAVLVLALGLREVRREAPAAGRPILGSFRTMPPP
                     FRRFLLIFALFTLGNSADAFIILRAQERGLSVLGVLAMLLAFNAVYTAVAGPAGRLSD
                     RIGRPRLILAGWAVYFAIYLGLALAGAAWQVVALYVVYGIYHGLVEGNARAFVADLAG
                     AEGRGAAYGLYHAVVGLMALPASLIGGILWQGIGSWAGLGPSAPFLFGAILAGAAALL
                     FATLLPPRRWQPGRA"
     gene            1368..1910
                     /locus_tag="PK829_11810"
     CDS             1368..1910
                     /locus_tag="PK829_11810"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41943.1"
                     /translation="MNHDDHVALLREGVHRRGAGAVWADLGAGDGAFTLALADLLGDG
                     ATVHTVDRDGAALARGAAEARRRFPGVQVRPQTADFSRPLALPPLDGLVMANSLHFVR
                     DGDKAALLRRVVAHLRADGHFLLVEYDADRGNLWVPHPLSFDAWSALAARVGLARPVL
                     LGRRPSRFLRAIYAAAAGLA"
     gene            2046..2522
                     /locus_tag="PK829_11815"
     CDS             2046..2522
                     /locus_tag="PK829_11815"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOU41944.1"
                     /translation="MKTINRNQLKDKAMSRMHDLRQAVSAHWREMVEAEAPAGDMARA
                     FTAGTLVSTIPVPMVDMAIAAFVMRRFSRLPRAPFFAAMALTNNVVMAPLYAATPKLG
                     ALAIGWMALHTPVALPEALVLRVLIGYLLIAAGLGLGSFVLANTGFWGYQRARRSA"
     gene            2604..>3027
                     /locus_tag="PK829_11820"
     CDS             2604..>3027
                     /locus_tag="PK829_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU41945.1"
                     /translation="MPTHYQGSEAERRALDTYIKLTRAAEAVNVHVNERLHTSGLTVS
                     QFGVLEALYFRGPMPVGQLAEKILRSSANLTLVVDNLVRRGLVERARRADDRRCVDVS
                     LSAEGRALIVGLMPGHVAGVVDALAALSAEEQLTLAALC"
BASE COUNT          397 a         1061 c         1045 g          524 t
ORIGIN      
        1 gggcgagggc gccaacgtgg tcagccaggt gcatcagtat ttgcggacgg tgtagctgaa
       61 cagtcagaag ggagagagaa acaaagccct gcagtggctc tctttctccc ttgctcctgc
      121 tccccagcgc tcgggcgcaa gctcaacatg aaaaccaaaa tcccccgcaa tatctgggtc
      181 atgacggcgg ccagcttcct gaccgacgtg tccagcgaga tggtcaacaa cctcgtgccg
      241 ctgttcctgg ccaacgtgct gggggcgcgg acgggaacca ttggcctggt ggagggcgtg
      301 gtggagtcga tgagcagctt tatcaagctg ctgtcgggct ggtggtcaga tcgcgtggtc
      361 agccgcaagc cgccgacggt catcggctac gccctgtcgg ccgtctccaa gccgctgctg
      421 gccttcgccg ccagctggac agccgtgctg ggagcgcgct tcggcgaccg catgggcaag
      481 ggcatccgca cggccccgcg cgatgctctg ctggccgaca gcgtgcccgc cgaggggcgc
      541 ggcgcggcct tcggcttcca ccgcgccggc gacagcctcg gcgcggtcgg cgggctggcg
      601 ctggcgctgc tggccgtctg gctgatccag ggcaatgccc tgtcgctgag ccgggccacc
      661 ttccagcgca ttgccctgtt cagcgccgtg cccgcccttc tggccgtgct ggtgctggcc
      721 ctggggctgc gcgaggtgcg gcgcgaagcc ccggccgccg gtcgccccat tctcggctcc
      781 ttccggacca tgccgccgcc cttccgccgc ttcttgctca tcttcgccct gttcacgttg
      841 ggcaactcgg ccgatgcctt catcatcctc cgggcacagg agcgcgggct gagcgtcctg
      901 ggcgtgctgg ccatgctgct ggctttcaac gccgtctata cggccgtggc cgggccggcc
      961 gggcggctgt cggaccgcat cggccggccg cggctcatcc tggccggctg ggcggtctat
     1021 ttcgccatct acctggggct ggccctggct ggcgcggcct ggcaagtggt ggccctctac
     1081 gtcgtttacg gcatctacca cgggctggta gagggcaacg cgcgtgcctt cgtggccgac
     1141 ctggccggcg cagaggggcg cggcgcggct tatggcctct accatgccgt cgttggcctg
     1201 atggccctgc cggccagcct catcggcggc atcctctggc agggcatcgg cagctgggcc
     1261 ggcctgggcc cgtcggcgcc gttcctcttc ggcgcgatcc tggccggcgc ggcggcgctg
     1321 ctgttcgcca cgctgctgcc gccgcggcgt tggcagcccg ggcgcgcatg aaccacgatg
     1381 accatgtcgc tctgctgcgc gagggcgtcc accggcgcgg cgccggagcc gtctgggccg
     1441 atctgggcgc gggcgatggc gctttcacgc tggccctggc cgacctgctg ggcgacggcg
     1501 cgaccgtcca cacggtcgac cgcgacggcg cggccctggc ccgcggcgcg gccgaggcgc
     1561 ggcgacgctt ccccggggtg caggtgcgcc cgcagacagc tgacttcagc cgccccctgg
     1621 ccctgccgcc gctcgatggc ctggtgatgg ccaactcgct ccactttgtc cgcgacggcg
     1681 acaaggcagc cctgctgcgc cgtgttgtcg cccacctgcg agcggacggc cacttcttgc
     1741 tggtcgagta cgatgctgac cgggggaacc tctgggttcc ccacccgctg tcgttcgacg
     1801 cctggtcggc gctggccgcg cgggtgggtc tggcgcggcc ggtgctcctc ggccgtcgcc
     1861 ccagccgctt cctgcgggcc atctacgccg ccgccgccgg cctggcgtag gtttccctac
     1921 gccgggccaa cgcccgcccg tcgccacccc cacgcccccc taaaaggcgg aactaccctc
     1981 cccgccccgt cgtttccctc atgtaaggcc gcgcaaccaa catccggcgg cccgcgagga
     2041 aaacgatgaa gacaattaat cgcaaccaac tgaaagacaa ggccatgagc cggatgcatg
     2101 acctgcggca agcggtctcg gcccactggc gcgagatggt cgaggccgag gccccggctg
     2161 gcgacatggc ccgcgccttc acggccggca cgctggtcag caccatcccc gtgccgatgg
     2221 tcgatatggc catcgccgcc ttcgtgatgc gccgcttctc gcggctgccg cgcgcgcctt
     2281 tcttcgccgc catggccctg accaacaacg tcgtcatggc cccgctctac gccgccacgc
     2341 ccaagctcgg cgcgctggcc atcggctgga tggcactgca tacgccggtg gccctgccgg
     2401 aggcgctggt gctgcgcgtg ctgattggct atctgttgat tgccgccggc ctggggctgg
     2461 gcagcttcgt tctggccaac actggcttct ggggctacca gcgggcgcgg cggtcggctt
     2521 agccatcgcc ccaatcgtct tgacagcagc gccagggcag gctattatta tgacatcaaa
     2581 ctattcgata tggaaagaat gcgatgccaa cacactacca gggcagcgag gccgagcgcc
     2641 gggcgctgga cacctacatc aagctgacgc gggcggctga ggcggtcaac gtccacgtta
     2701 atgaacgcct tcacacctcc gggctgacgg tcagtcagtt tggggtgctg gaggcgcttt
     2761 actttcgcgg gccgatgccg gttgggcagc tggccgagaa gattttgcgc agcagcgcta
     2821 acctgacgtt ggtggtggat aatctggtcc ggcgcggcct ggtggagcgg gcgcgccggg
     2881 cggacgaccg ccgctgtgtg gatgtcagtc tgagcgcgga ggggcgagcg ctgatcgtcg
     2941 gactgatgcc gggccacgtg gccggcgtgg tagatgcctt ggcggcgcta tcggccgagg
     3001 agcagcttac gctggcggcg ctctgtc
//
LOCUS       DAOSMQ010000397         3026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_32502_length_3026_cov_4.195220, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000397 DAOSMQ010000000
VERSION     DAOSMQ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32502_length_3026_cov_4.195220"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2360
                     /locus_tag="PK829_11825"
     CDS             <1..2360
                     /locus_tag="PK829_11825"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU41946.1"
                     /translation="RLNFSRAILVRELMLAQGDGGTAIWAGNWGWNSLPVGWAGRPSI
                     WGQTTAEAQAAQTVAALERARREWPWMGLMFLENWTAAPAGTAADDPRWGFSIAGRPA
                     AEALAGYQAGQLPSVAAPGFHLARSDDPAQVYSGAWAFSPQFGADIGQSGDRVTFTFW
                     GTDIGLRVRRADFRARLYATVDGQPANALPRDENGAALVLTAGDPSEDFLSDEWIAHG
                     LAPGVHTLELTASRGWDQWALNGFAVAYRPEGLGPAWLRPALAVLGALLAALAALAGR
                     RAAWGLAWAGAGRAYRRLSDRGQLLVLAALAALATLTGWLTWGPDALGVYRRLGDLGQ
                     LAATGGAAALFYVSPSAVLYVLALAALVVLLTLRPAWGLALIALAMPFYVPQTTKPVF
                     GYRFSPVEVFTLATLAAWAARTALDAGRDYRRTGALPARPRWQRADWAALVFVVVATV
                     SLFFAQRRGVALTEWRVVILEPALFYLLLRAIRPTGRELWVILDAWVLSGVLVAGYGL
                     WQYATGQNLIAAEGGLLRLRSIYGSPNNVALYLDRLLPLLAAVPLLGGALSPRRRWVY
                     GLALLPTGLALLLTFSKGAFLLGVPAGLLVVFWVWQRRAGRRAWPWVIGAGAAGVVAL
                     AVASRVPALAARLDLFGATGFFRVNLWRAALHMAVDHPFFGVGLDNFLYAYRGAYILD
                     AAWQEPNLNHPHNIVLDFATRLGFVGLLAGAWLIGEAGLASGRALRRASTAWRPVAAG
                     LAGGLAAMLAHGLVDHSFFLVDLAFVFYLILGSAVWLATEPAAAD"
     gene            complement(2357..>3026)
                     /locus_tag="PK829_11830"
     CDS             complement(2357..>3026)
                     /locus_tag="PK829_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU41947.1"
                     /translation="HTRITLITSLSLVVVGTVLFYLTEARHGSVFGGESAGRLWLVSF
                     FHSVASRTSGFVISGQFGQLAPANGLLLTVLMFIGASPASTGGGITTSTFAVLLLAMW
                     NFARGRSEIRAGRRTISLDILFKAAAIITGAALVISVVTWLLLYTQENVTLVAALVEA
                     VSAFSTTGFSLGLTPRLNLFGQLLIAGLMFFGRAGTLTLIVALARPRVDPPVGYPQER
                     ILIG"
BASE COUNT          396 a         1043 c         1083 g          504 t
ORIGIN      
        1 agcgcctcaa cttcagccgc gccatcctcg tccgcgaatt gatgctggcc cagggagacg
       61 ggggcacagc gatttgggcg ggcaattggg gctggaacag cctgcccgtg ggctgggccg
      121 gccggccgtc catctggggg cagacgactg ccgaggcgca agccgcgcaa accgtcgccg
      181 cgctggaacg cgcccggcgg gagtggccgt ggatgggcct gatgttcctg gagaactgga
      241 cggccgctcc ggcgggcaca gctgccgacg atccgcgctg ggggtttagc attgccggac
      301 ggccggcggc cgaggccctg gctggctatc aggcggggca actaccgagc gtagccgcgc
      361 ccggcttcca cctggcccgc tccgacgacc cggcgcaggt ctacagcggc gcgtgggcgt
      421 tctcgccgca gttcggggcc gacatcggcc agagcggcga ccgcgtgacc ttcaccttct
      481 ggggcactga catcggcctg cgcgtgcgcc gggccgactt ccgcgctcgc ctctacgcca
      541 cggtcgacgg ccagccggcc aacgccctgc cgcgcgacga gaatggcgcg gcgcttgttc
      601 tcaccgctgg cgacccatcc gaggacttcc tctccgacga gtggatcgcc cacggcctgg
      661 cgccgggcgt ccatacgctg gagttgacgg cctcgcgcgg ctgggaccag tgggcgctca
      721 atggcttcgc cgtagcctac cggccggagg ggctgggacc ggcgtggctg cggccggcgt
      781 tggccgtgct gggggcattg ctggcggcgc tggcggcgct ggccgggcga cgggcagcgt
      841 ggggcctggc ctgggccggc gctggccggg cctatcgtcg cctcagcgac cgcggccagc
      901 tccttgtcct cgccgcgctg gctgcgctgg ccacgctgac cggttggctg acgtgggggc
      961 cggacgcgct gggcgtctat cgccgtctgg gcgacctcgg ccagttggcg gcgacaggcg
     1021 gggcagctgc ccttttctac gtgtcgccat cggctgtgct ctacgtcctg gcgctggcgg
     1081 cgctcgtcgt cctgctgacg ctgcggccag cctggggcct ggcgctcatc gccctggcca
     1141 tgcccttcta tgtcccgcag acgaccaagc cggtgttcgg ctatcgcttc tcgccggtgg
     1201 aggttttcac actggccacg ctggccgcct gggcggcgcg gacagcccta gacgccgggc
     1261 gggactaccg ccgcaccggc gcgttgccgg cgcgtccccg ttggcaacgg gccgactggg
     1321 ccgcgctggt cttcgtcgtc gtggccacgg tctcactctt cttcgcccaa cggcgcggtg
     1381 tagccctgac cgagtggcgc gtcgtcatcc tggagccggc gctgttctac ttgctgctgc
     1441 gcgccatccg gccgacgggg cgggagttgt gggttattct cgacgcctgg gtgctcagtg
     1501 gcgtgctcgt ggccggctac ggcctgtggc aatacgctac tggccagaat ctcatcgccg
     1561 ccgagggcgg cttgctgcgg ctgcgctcta tctacggctc gcccaacaac gtcgccctct
     1621 atctcgaccg cctgctacca ctgctggcgg ccgtccccct gctcggcggg gcgttgtcgc
     1681 cgcggcggcg ttgggtctat ggtctggccc tgctgcccac cggcctggcg ctgctgctga
     1741 cgtttagcaa gggcgcgttc ctgctgggtg tgcccgcggg gctgctggtc gtcttctggg
     1801 tctggcagcg gcgggcgggg cggcgggcgt ggccgtgggt catcggcgca ggcgcggccg
     1861 gcgtggtggc gctggccgtg gccagccgcg tgccggcgtt ggcggcccgg ctcgacctgt
     1921 tcggggccac aggctttttc cgggtcaacc tgtggcgggc ggcgctccat atggccgtcg
     1981 atcacccctt ctttggcgtc ggactggaca acttcctcta cgcctaccgc ggcgcgtaca
     2041 ttctcgacgc cgcctggcaa gaaccgaacc tcaatcaccc tcacaacatc gtcctcgact
     2101 tcgccacgcg gctgggattc gtcgggctgc tggcgggcgc gtggctcatc ggcgaggcgg
     2161 ggctggcgtc agggcgggcg ctgcgccgcg ccagcacggc gtggcgaccg gtggccgccg
     2221 gcctggctgg cgggctggcg gccatgctgg cccacggcct ggtcgaccac agcttcttcc
     2281 tcgtcgatct ggccttcgtc ttctacctga tcctcggcag cgccgtctgg ctggcgacag
     2341 agccggccgc ggctgactaa ccgatgagga tgcgttcctg cggatagccc acgggcgggt
     2401 ccacccgcgg ccgggccagg gcgacaatga gcgtcagcgt tcccgcgcgg ccgaagaaca
     2461 tcagcccggc gatgagaagc tgcccgaaca ggttcagccg tggcgtcagc cccagcgaga
     2521 agccagtcgt ggagaaggct gagacggctt ccaccagcgc cgccaccagc gtaacgtttt
     2581 cctgtgtgta gagcagtagc caggtaacca cgctgataac caacgctgcg ccggtgatga
     2641 tcgccgcggc cttgaagagg atatccagcg agatcgtgcg ccggccagcg cgaatctcgc
     2701 tgcgaccgcg ggcgaagttc cacatagcca gcaacagcac ggcaaatgtg ctcgtggtaa
     2761 tgcccccgcc cgttgatgcc ggcgacgcgc caatgaacat gagtaccgtc agcaacaggc
     2821 cattggccgg cgccagctgg ccgaattggc cagaaataac aaagcctgat gtgcggctgg
     2881 caacagagtg gaagaacgac accagccaga gccgcccggc cgactcgccg ccgaagacgc
     2941 tgccgtgacg cgcctccgtc aggtagaaga gcaccgtccc gacaaccacg agcgacagtg
     3001 acgtgatcag ggtgatgcgt gtgtgc
//
LOCUS       DAOSMQ010000398         3015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_32658_length_3015_cov_4.555068, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000398 DAOSMQ010000000
VERSION     DAOSMQ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32658_length_3015_cov_4.555068"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PK829_11835"
     CDS             <1..405
                     /locus_tag="PK829_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41948.1"
                     /translation="RQTPLLMPAVNFAERTLALLPSSRLRVRALSAVYLILLASGVVP
                     LLLALFLVARYAPILQQPALVERVVATLVNVGRVAATVAGALLAGAGRFVVEQPAVVG
                     LIILLAGFVLLWGGVLQRLLAQPAPIRVRNRG"
     gene            447..1568
                     /locus_tag="PK829_11840"
     CDS             447..1568
                     /locus_tag="PK829_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41949.1"
                     /translation="MKRRILIPLALVLLLIVALASTASAGPLDDTVIEANEVVNNDVI
                     VLDGDLTVHEGAVVNGDVVVFNGDATIDGVVSGSVTLFNGDLRAGEAAVVEGECVLLN
                     GKISGPASLRNCTAVEDLKLPPMAIPAIPEIPAIPEVPAVPELPAAPEVPAVPVVPVM
                     PNRQVNEGPGFGARVVGVIASTLLFTLLGLFVGLVMPNQLRRIVSTARAKPVASGLAG
                     VLTAVAVPSLIVLLIPVSILLTLVCIGLLGFPIMILLALGLVLGSFLGWVVVGTWLGL
                     RLYGGKEGRIVRAAALGTGTLTFIFGVLGLLPLVFGESLLAFVIVSLGLGAVTLTQFG
                     MKPYPRQPRGGTSNDDLDPDKVDVVLGTLPPDEAAHQRA"
     gene            1842..>3015
                     /locus_tag="PK829_11845"
     CDS             1842..>3015
                     /locus_tag="PK829_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41950.1"
                     /translation="MSTTTVNREFRLENQRQFDQRSPVRWIASQLRHYWYLPLLAVIA
                     AGFNNFGASYIQLLIGRGFDLLNTPGWTNRSLLLLALAVFGSAAVQGIFGLVRNSAFE
                     FMAQRVERDSRDSLYASLLGKSQTFHNRQRIGDIMARATNDVRMLNIMFSPGLMLITD
                     SLVGIFAPIVLIARINTQLLLMPVVFVFLYIITIRDYVRRLSPISEGLRESFGTMNAG
                     LAEAVSGIEVVKGNAQEAQEADKFVGDATVYRDYAIREGEVQARYLPMLVFSLCWGAA
                     FFHAMLLWRDDALTLGQVVSYVGLFGFLRFPTFVSVFTFQVAQLGVASARRILELINT
                     ETELDENQSGVAQPIAGAVEFRHVSFGYAWRDSPESPARSDAGEWRDSPESPARSDS"
BASE COUNT          432 a         1024 c          960 g          599 t
ORIGIN      
        1 cggcagacgc cgctgctgat gccggcggtc aacttcgccg agcgcactct ggcgctgttg
       61 cccagcagcc ggctgcgcgt gcgggcgctg agcgccgtct acctgatcct gctggccagc
      121 ggcgtcgtgc cgctgctgct ggctctgttc ctggtcgccc gctatgcgcc catcctgcag
      181 cagccggcgc tggtcgagcg cgtcgtcgcc acgctggtga acgttggtcg cgtggccgcc
      241 acggtggccg gcgctctgct ggccggggcg ggccgctttg tagttgagca gccggccgtc
      301 gttggcctga tcatcctcct ggccggcttt gtcctgctgt ggggtggcgt tttgcagcgg
      361 ctgctggcgc aacccgcgcc tatccgcgtg cgtaatagag gataaaggac gtaggagtat
      421 tgggaacaac gctgaaggag attcgcatga agagacgtat cctgattcct ctggctctgg
      481 tgctactatt gatcgtcgcc ctggcgtcga cggcttccgc cggcccgctc gatgacaccg
      541 tcatcgaggc caacgaagtg gtcaacaatg acgtcatcgt cctcgatggc gatttgaccg
      601 ttcacgaagg cgcggtcgtc aacggcgacg tggtcgtctt caatggcgac gcgaccattg
      661 acggcgtcgt cagtggcagc gtgacgctgt tcaacggcga cctgcgcgcc ggcgaggccg
      721 ctgtcgtcga gggcgaatgc gtcctcctga acggcaagat cagcggtccg gcgtcgctgc
      781 gcaactgcac cgccgtcgaa gacctcaaac tgccgccgat ggcgattccg gctatcccgg
      841 agattccggc catccctgaa gttccggccg tgccggagct tccggccgcg ccagaggttc
      901 cggctgtgcc cgttgtgccg gtaatgccca accggcaggt aaatgagggg ccgggcttcg
      961 gcgcgcgcgt ggttggcgtc atcgccagca cgctgctgtt caccctgctg ggcctgttcg
     1021 tcggcctggt gatgcccaat cagttgcggc gcatcgtcag cacggcgcgg gccaagccgg
     1081 tagccagcgg gctggccggg gtgctgacgg ccgtggccgt gccttcgctc atcgtcctgc
     1141 tgattccggt gtcgatcctg ctgaccctgg tctgcatcgg tctgctcggc ttcccgatca
     1201 tgatcctgct ggccctgggg ctggtgctgg gcagcttcct gggctgggta gtcgtcggca
     1261 cgtggctggg cctgcgcctc tatggcggca aggaagggcg catcgttcgc gccgcggcgc
     1321 tgggcacagg cacgctgacg ttcatcttcg gcgtactggg cctgttgccg ctggttttcg
     1381 gcgaatcgct gctggccttc gtcatcgtct ccctgggcct gggcgcggtc acgctgacgc
     1441 agtttggcat gaagccctat ccgcgccagc cgcgcggcgg cacgtcgaac gacgacctgg
     1501 acccggacaa ggtcgatgtc gtcctgggca cgctgccgcc cgacgaggcc gcccaccagc
     1561 gcgcctaaca gctatagaaa tcgagtcttt gtgaaaacaa aaggacgccg gggaggcatt
     1621 gcttccccgg cgtttttcgt tcaacaagcg ctaacccgcc ggccagccct cggcctccag
     1681 ttcggccggc aaccactcct ggcaagggtt gaatcctgtt ttagtgttgc tgccaccggc
     1741 agccgggtct tgtgctgctg tctgaagtcg tctataatgg ccgttcgttt agaacgtttg
     1801 tttgctcttg gccggcctgc ccggctcctg gaagacccgc tatgagtacg accacggtca
     1861 accgcgaatt tcgcctggag aaccagcggc agttcgacca gcgcagcccg gtgcgctgga
     1921 ttgcctcgca actgcgccac tactggtatc tgccgctgct ggccgtcatc gccgccggct
     1981 tcaacaactt cggcgccagc tacatccagc tgctcatcgg ccgcggcttc gacctgctca
     2041 acacgcccgg ctggaccaac cgttcgctgc tgctgctggc cctggccgtg ttcggctcgg
     2101 ccgccgtgca gggcatcttc ggcctcgtcc gcaactccgc cttcgagttc atggcccagc
     2161 gcgtcgagcg cgactcccgc gattcgctct acgccagcct gttgggcaag agccagactt
     2221 tccacaatcg ccagcgcatc ggcgacatca tggcccgcgc caccaacgac gtgcgcatgc
     2281 tcaacatcat gttcagcccc ggcctgatgc tcatcaccga ctcgctcgtc ggcatctttg
     2341 cccccatcgt cctcatcgcc cgtatcaaca cgcagttgct gctcatgccg gtcgtcttcg
     2401 tgttcctcta catcatcacc attcgcgact atgtgcgccg cctcagcccc atcagcgagg
     2461 ggctgcgcga gtcgttcggc accatgaatg ccgggctggc cgaggccgtc agcggcatcg
     2521 aggtggtcaa gggcaacgcc caggaggccc aggaggcgga taagttcgtg ggcgacgcga
     2581 ccgtctaccg cgactacgcc atccgcgagg gcgaagtgca ggcccgctat ttgcccatgc
     2641 tcgtcttcag cttgtgctgg ggcgcggcct tcttccacgc catgctcctc tggcgcgacg
     2701 acgccctgac gctgggccag gtcgtcagct acgtcggcct gttcggcttc ctgcgcttcc
     2761 ccaccttcgt gtccgtcttc accttccagg tggcccagtt gggcgtcgcc agcgcccggc
     2821 gcatcctgga actgatcaac accgaaaccg aactggacga gaaccagagc ggcgtcgccc
     2881 agcccatcgc cggcgcggtt gagttccgcc acgtcagctt cggctacgcg tggcgcgatt
     2941 ctcccgaatc gccggcgcgg agcgatgcgg gagagtggcg cgattctccc gaatcgccgg
     3001 cgcggagcga ttcgg
//
LOCUS       DAOSMQ010000399         3014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_32673_length_3014_cov_4.175059, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000399 DAOSMQ010000000
VERSION     DAOSMQ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32673_length_3014_cov_4.175059"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK829_11850"
     CDS             complement(<1..385)
                     /locus_tag="PK829_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41951.1"
                     /translation="MNQLTRFNALKRLFLAVLLVGVLLLVSGCASPGRSAATAAATPP
                     APATAASSPTPAASATPPPPPATPEPAAQPTAEPSATATATASATPTATATATASLAA
                     DGPLVLLYAPDGHLYRGAVETGLGRR"
     gene            700..2289
                     /gene="dnaX"
                     /locus_tag="PK829_11855"
     CDS             700..2289
                     /gene="dnaX"
                     /locus_tag="PK829_11855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOU41952.1"
                     /translation="MSQALYRKWRPARFDQVIGQEHVTRTLQAAVAADRVGHAYLFCG
                     PRGTGKTTMARLLAKAVNCEAADPAVRPDDTCPLCRAVNEGRFLDLIEIDAASNTGVD
                     DIRTLREKVNFAPSQGRYKVYIIDEVHMLSIAAFNALLKTLEEPPAHTIFVLATTEEH
                     KVPVTIKSRCQQFNFRLLTTPEIAARLNWLAGQETLAIEPGAVELLARQASGSLRDAE
                     SLLDQLIVSPDDRITLERAQLVLGTAPDAAVLAVVDAWLDADSAHGLGVIHDALSSGA
                     DARQFCRQMVAHLRQLLLLQVAGAQLDVEGPVERKAEMLAQAGRAPRRALIDAVRRFQ
                     EAALQPAGSWQPQLPLELAFIELLPDAPMAIPRAEERPPAADRRPPQVEERPPTADRR
                     PPRAEEPPPRAEEPPPAAPRPPTPAAVLTVAAVAGMWPEMTQQVGRQNKNLPALLTMC
                     KPLAIEGRTIILGFDYPLLKDKFDKTQGAADLVVAAFRALSGTDCLIRTVATADYPVP
                     IHRDEFQALATELGGVVRDDE"
     gene            2391..2762
                     /locus_tag="PK829_11860"
     CDS             2391..2762
                     /locus_tag="PK829_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOU41953.1"
                     /translation="MTKRNFHGKPKKKTSGNPNDMLAQVQKMQQDMATAQTALENETL
                     TVSAGGGAITITITGHQRVRSIAIDKELLTADEAEFLQDMLVAGINAAIEQSQALAAQ
                     RMEGITGGVGGMDGLLGGLMG"
     gene            2767..>3014
                     /locus_tag="PK829_11865"
     CDS             2767..>3014
                     /locus_tag="PK829_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HOU41954.1"
                     /translation="MAQSVLPRSVQRLIDEFARLPGIGPKSASRLTFYLLRGSDNQAL
                     DLAVALQELKERTRLCSVCFNITEDDPCPLCSDGARDAG"
BASE COUNT          517 a         1045 c         1024 g          428 t
ORIGIN      
        1 ggcggcggcc gaggccggtt tcgaccgcgc cgcggtagag atggccgtcc ggggcgtaga
       61 ggaggacgag cgggccgtcg gcagccaaag acgcggtcgc cgtggcggtc gctgtggggg
      121 tggctgaggc ggtcgccgtg gcggtcgcgg atggctcggc cgtgggctgg gctgccggtt
      181 cgggggtggc gggcggcggt gggggcgtgg cgctggcggc cggggtgggg ctgctcgccg
      241 ccgtcgccgg ggctgggggc gtggccgccg ccgtggccgc gctccggccc ggcgacgcgc
      301 agccggagac aagtagcagc acaccaacga gaagcacagc cagaaaaagc cgcttgagag
      361 cgttgaagcg tgttagctgg ttcatgatct tccctctatt cgccgggcag gaaacggcgt
      421 aaccgacagc ttcgactgct ctgatcatag ccgcgccctt gccccgctga tggacggccg
      481 ggccacggct gccctacaag tagccgacgg cccagggcct ttcagattac gccgcccgta
      541 gcacagcctg ttaggctgtg gccaaccacg acagcctaac aggctgtgct acgggcggcg
      601 cgtgttgtta ccgaccccca aaagtggtaa acttcggcat ccagacctca gaggtcttct
      661 gagacctctg aggtctagcg gcctactacc gggaagctca tgtcgcaagc cctctaccgc
      721 aaatggcggc cggcccggtt cgaccaggtg atcggccagg aacacgtgac ccgcacgctg
      781 caggccgcgg tggcggccga ccgcgtgggc cacgcctacc tgttctgcgg cccgcgcggc
      841 acgggcaaga cgacgatggc ccggctgctg gccaaggcgg tcaactgcga ggccgccgac
      901 cccgccgttc gccccgacga cacctgtccc ctctgtcgcg ccgtcaacga aggccgcttc
      961 ctcgacctga tcgagatcga cgcggccagc aacaccggcg ttgacgacat ccgcaccctg
     1021 cgcgagaagg tcaacttcgc ccccagccag gggcgctaca aggtctacat catcgacgaa
     1081 gtccacatgc tctccatcgc cgccttcaac gccctgttga agacgctgga ggagccgccg
     1141 gcccacacca tcttcgtgct ggccacgacc gaggaacaca aagttcccgt tacgatcaag
     1201 tcgcgctgcc agcagttcaa cttccggctg ctgacgacgc cggagatcgc cgcgcggctc
     1261 aactggctgg ccgggcagga gacgctggcc atcgagccgg gcgcggtgga gctgctggcc
     1321 cggcaggcgt cgggcagcct gcgcgacgcc gagagcttgc tcgaccagct tatcgtctcg
     1381 cccgacgacc gcattaccct ggaacgggcg caactggtgc tgggcacggc cccagacgcg
     1441 gccgtgctgg ccgtcgtcga tgcctggctc gacgccgata gcgcccacgg cctgggcgtc
     1501 atccacgacg ccctgtcctc cggcgccgac gcgcgccagt tctgccggca gatggtcgcc
     1561 cacctgcgcc aactgctgct gttgcaagtg gccggggcgc aactggacgt ggagggaccg
     1621 gtggagcgca aggcggagat gctggcgcag gctggccgcg cgccacgccg ggcgctcatc
     1681 gacgccgtgc gccgcttcca ggaggcggcg ctgcaaccgg ctggtagctg gcagccgcag
     1741 ttgccgctgg aactggcttt catcgaactg ctgcccgacg cgccgatggc catcccgcga
     1801 gcggaggaac gaccgccggc ggccgaccgc cgaccgccgc aagtggaaga acgaccgccg
     1861 acggccgacc gccgaccgcc acgggcggaa gaaccaccgc cacgggcgga agagccaccg
     1921 ccggccgcgc cccgtccccc gacgcccgcg gccgtgctga cggtcgcggc cgtggccggc
     1981 atgtggccgg agatgaccca gcaggtcggc cggcagaaca agaacctgcc cgcgctgctg
     2041 accatgtgca agccgctggc catcgagggc cggacgatca tcctcggctt cgattacccg
     2101 ctgctgaagg acaaattcga caagacccag ggcgcggccg acctggttgt ggccgccttc
     2161 cgcgccctca gcggcacgga ctgcctcatc cgcacggtgg ccacggccga ctaccctgtg
     2221 cccatccacc gcgacgagtt ccaggctctg gcgaccgaac tcggcggcgt ggtgcgagac
     2281 gatgaatgaa aaatctcacg caaagacgca aagtacgcca agctcagaat tctgagcttg
     2341 gcgtactttg cgtctttgcg tgaggtcttc cttcctaaat cgaggtacac atgaccaagc
     2401 gtaatttcca cggcaaaccg aagaagaaga cgagcggcaa ccccaacgac atgctggccc
     2461 aggtgcagaa gatgcagcag gacatggcca cggcgcagac ggcgctggag aacgagacgc
     2521 tgaccgtcag cgccggcggc ggagccatca ccatcaccat caccggccac cagcgcgtgc
     2581 gcagcatcgc catcgacaag gagctgctga cggccgacga ggccgagttc ctgcaagaca
     2641 tgctcgtggc cggcatcaac gccgccattg agcagtcgca ggcgctggcg gcccagcgca
     2701 tggagggcat taccggcggc gtgggcggga tggacgggct gctgggcggc ctgatggggt
     2761 aagcccgtgg ctcaatctgt actgccccgc tcggtccaac ggctcattga cgagttcgcc
     2821 cgcctgcccg gcatcggccc caagtcggcc tcgcgcctga ccttctacct gctgcgcggc
     2881 agcgacaatc aggccctcga cctggccgtg gccctgcaag agttgaagga gcgcacgcgc
     2941 ctgtgttcgg tgtgcttcaa tatcaccgag gacgacccct gtcccctgtg cagcgacggc
     3001 gcgcgcgacg ccgg
//
LOCUS       DAOSMQ010000400         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_32824_length_3003_cov_3.951832, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000400 DAOSMQ010000000
VERSION     DAOSMQ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32824_length_3003_cov_3.951832"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PK829_11870"
     CDS             complement(<1..702)
                     /locus_tag="PK829_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41955.1"
                     /translation="MKKFVPLLLLALMLLAAPAGAQSSPGADMPNANDWPTVALPALD
                     LTAPGNAPATTPHLDSALATLAAQAPERAAPAAESLGLRVEDGQVQARLVVAPGGEAA
                     ARAAVEAAGGRVTGALADALQVWLPTGALTTVAASAGVAVLGAPDYAQLAEVTSEGVA
                     RAGADAWHAAGLRGQGVRVAIIDAGFQGYSAKLSTELPAAVVAKNFVDGQPDAEVDAT
                     TAHGTACAEIVHDMAP"
     gene            complement(712..1881)
                     /locus_tag="PK829_11875"
     CDS             complement(712..1881)
                     /locus_tag="PK829_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOU41956.1"
                     /translation="MIYIDISAAVHSKAGLGRYSERLAAALIARRPERYALFYNRGSG
                     AAEPAGLPAGVPRRSVRWGYKPWRMAVLLGQMTGVGFDRLVPGAELFHSTEHLLFPLR
                     GAPTVLTVHDLIFKRFPEYHKSLNYWYLNRAMPLYCRRATAIVAVSEATKRDLVQFYG
                     VDPAKVTVVYEAAASHFQPPSPARIAAVRDSYRLPQRFLIHLSTIEPRKNLLRLLDAF
                     QILRRSFPDLHLLLAGARGWLDADVFARIDADGLGAVVRPLGWVPDDDLPAIIGAAAL
                     AVQPSLYEGFGLPILEHMACGQVVAASDSSSHPEVGGAAAAYFDPTDVVGMAAVIARL
                     LSDGDEYARRRALGLAQAAQFSWARAAAETEAIYDRLLSPTEGRVRDAANETRVG"
     gene            complement(1912..2721)
                     /locus_tag="PK829_11880"
     CDS             complement(1912..2721)
                     /locus_tag="PK829_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU41957.1"
                     /translation="MPKQPTAYHRPRSVDAALNLLRQPNTVPLAGGTALLATEEGIDA
                     AVVDLQDAGLNNITWADDGRVLRLGAMTRLADLDAVLAPLSDLQGAAGLLRAAIHRAG
                     PNTYRHAATAGGVIASRLPDSEFLAALLALGTTVNLRLPSPETLSLAAYLADDDRPPG
                     LITEVDVYWPQGRGAAERVARTPADAPIVSVTLWRPDGGAVRLAATGISPRPMRLRAA
                     EAALGDGTAEAVAAAAEAARAAALHPGDFRGDAAYRAEMAAVLTRRVWQSL"
BASE COUNT          452 a          997 c         1122 g          432 t
ORIGIN      
        1 tggggccatg tcgtgaacga tctcggcgca ggcggtgcca tgggcggtgg tggcgtctac
       61 ctcggcgtcg ggctggccgt cgacgaagtt cttggcgacg accgcggccg gcagctccgt
      121 gctgagcttg gcgctgtaac cctggaagcc ggcgtcgatg atggcgacgc gcacgccctg
      181 gccgcgcagc ccggcggcgt gccaggcgtc ggcgccggcg cgggccacgc cttcggaggt
      241 gacttccgcc aattgcgcgt agtccggtgc gccaagaacg gccacgcccg cagacgcagc
      301 cacggtcgtc agcgcgccgg tcggcagcca gacctgcagg gcatcagcca gcgcgccggt
      361 gacacggccg ccggcggcct ctacagcggc gcgcgccgcc gcctcgccgc cgggagcgac
      421 gaccagccgc gcctgcacct ggccgtcctc gacgcgcagg ccgagcgatt cggccgccgg
      481 ggcggcgcgc tccggcgctt gggccgccag cgtcgccagg gcgctgtcca gatggggcgt
      541 cgttgccggc gcattgccgg gcgcagtcag gtcgagggcg ggcagggcca ccgttggcca
      601 gtcgttggcg ttgggcatgt cagcgccggg cgacgattgc gcgcccgccg gggcggccag
      661 cagcatcagg gccagcaaca ggagggggac gaacttcttc atagctgagt tttaccccac
      721 acgcgtttcg ttggccgcgt ccctcactcg cccttcagtt ggcgacagga ggcggtcgta
      781 gatggcttcg gtctcggccg ccgcccgcgc ccaactgaat tgcgccgcct gggccaggcc
      841 cagcgcgcgc cggcgggcgt attcgtcgcc gtcgctcagc aggcgggcga tgacggcggc
      901 catgcccacg acgtcggtcg ggtcgaagta ggccgccgcc gcgccgccca cttcagggtg
      961 gctgctgctg tcgctggcgg ccacgacctg gccgcaggcc atgtgctcca gaatgggcag
     1021 gccgaaaccc tcgtagaggc tgggctggac ggccagcgct gccgcgccaa tgatcgccgg
     1081 caggtcgtcg tcgggcaccc agcccagcgg ccggacgaca gcgcccagcc cgtcggcgtc
     1141 gatacgagcg aacacatctg cgtcgagcca cccccgcgcc ccggccagga ggaggtgaag
     1201 atctggaaag gaacggcgta gaatctggaa ggcgtccaac aggcgcaaca ggtttttgcg
     1261 cggctcgatg gtgctcaggt ggatgaggaa gcgttggggc aggcggtagc tgtcgcggac
     1321 agcggcgatc cgggccgggg aggggggctg aaaatgggac gcggcggcct catacacaac
     1381 cgtcacttta gccggatcga cgccgtagaa ctggacgaga tcgcgcttgg ttgcctccga
     1441 cacggcgacg atggccgtcg cccggcggca atagagcggc atggcccggt tgagatacca
     1501 gtagttgagc gacttgtggt attccgggaa gcgtttgaag atgaggtcgt ggacggtcag
     1561 cactgtcggc gcgccgcgca gggggaagag caggtgttcg gtgctatgga acagctccgc
     1621 gccgggcaca agacggtcga aaccgacgcc ggtcatttgc cccagcagca cggccatccg
     1681 ccacggcttg tagccccagc gcaccgagcg ccggggcact cccgcgggca gcccggccgg
     1741 ctcggccgcg ccgcttcccc ggttgtagaa cagggcgtaa cgctccggcc gccgggcgat
     1801 gagggccgcc gccaggcgct cgctgtagcg ccccaggccg gccttgctgt ggacggcggc
     1861 ggagatgtcg atgtagatca tgggtggtag agacgtgccg gcggcgcgtc tctacaatga
     1921 ctgccagacc cgtcgcgtca gcaccgcggc catttcggcg cgataggcgg cgtcgccgcg
     1981 gaagtcgccg gggtggaggg cggcggcgcg agcggcctcg gcggcggcgg cgacggcctc
     2041 agccgtgccg tcgcccagag ccgcctcggc cgcgcgcagg cgcatgggcc gcgggctgat
     2101 gcccgtcgcc gccagccgga ccgcgccgcc gtcgggccgc cacagcgtta cggagacgat
     2161 gggggcgtca gcgggcgtgc gggccacgcg ctcggccgcg ccgcgcccct gcggccagta
     2221 aacatcgacc tcggtgatca gccccggcgg ccggtcgtcg tcggccaggt aggcggccag
     2281 actaagcgtc tccggcgatg gcaggcgcag gttcaccgtc gtgcccagcg ccagcagcgc
     2341 ggccaggaat tcgctgtcgg gcagccgcga ggcgatgacg ccgccggcgg tggcggcgtg
     2401 gcggtaggtg ttcggcccgg cgcggtgaat ggcggcccgc agcaggccgg ccgcgccctg
     2461 caggtcgctc agcggggcca gcacggcgtc gagatcggcc aggcgcgtca tcgcgcccag
     2521 gcgcaacacg cggccatcgt cggcccaggt gatgttgttc aggccggcgt cttgcaagtc
     2581 caccacggcg gcgtcgatgc cctcttctgt cgccagcagg gctgtgccgc cagccagggg
     2641 cacggtattg ggctggcgca gcaagttgag ggccgcatcg acgctgcggg gacggtggta
     2701 agcagtgggt tgtttgggca tatgctccct ctggggccag atgttggcca ggattgcgtc
     2761 catcttagcc cgcactcggc gatttgtcat cttgacaaca accggccagg gcctacaata
     2821 gccacattaa cgccagatga gtgcggacgc gcccgcgtgg agctaaagag cgcaacgatc
     2881 tgcgtccggc agcgtattga acatagaaat ggtggctcaa agattgtggg ccaacaaacg
     2941 tttgtccgag agagtgaatt cctatgttcg aagcgattag cggcctgagc gccgctttcg
     3001 gcc
//
LOCUS       DAOSMQ010000401         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_33004_length_2990_cov_4.623509, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000401 DAOSMQ010000000
VERSION     DAOSMQ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33004_length_2990_cov_4.623509"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2956)
                     /locus_tag="PK829_11885"
     CDS             complement(<1..2956)
                     /locus_tag="PK829_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU41958.1"
                     /translation="MWLPLASALPFSHNEQCVVSLRATAIHDADSDDPPDTLVADYTW
                     RFRIVPAPAGGILINELDSDTPGSDTAEFVELYDGGAGETDLSGLVLVFFNGTDDRAY
                     FAADLDGVRTDEDGYAVAGSTALGGALTLPAAMIQNGPDAIALYEGDAADFPNGTALT
                     TAGLRDAIVYGTSDPTDTGLLVLLAAGQAQVDENARAAAEADSSGRCPDGAGGRRYTA
                     TYRQNAPTPGRANDCHADVPPAVATVSPADGADGVTAQTQLEVVFSEAVTLDNNWFSI
                     VCDVSGSHAAASAGGPTTFILTPTLPFSHGEQCATTLRAAAVHDADSDDPPDTLAADY
                     TWRFRVAPAPAGGILINELDSDTSGSDTAEFVELYDGGTGGTDLSGLVLVLFNGKDDR
                     AYFAVDLDGARTNGDGYAIIGGPALGGVATLPAAVIQNGPDAVALYEGDAADFPSGTA
                     ITTAGLRDAIVYGTSDPTDNGLLVLLAAGQAQVDENARAAAESDSSGRCPDGAGLPRQ
                     TIAYLQNTPTPNAPNNCRVDAPPVIEAVSPANGAEHVSALAQLRVQFSEPVALDSNWF
                     TITCSVSGSHAADTGGGPMTFTLTPALPFADAEECTVTLGATAVHDADPDDPPDHLPD
                     NYTWHFRIAPVATDGILMNEVDSDTPGQDTAEFIELYDGGRGDTDLSGLVVVLWNGQD
                     DTAYQAIDLAGQQTDAEGYFVLGSATLPGVDWPLADATLQNGPDAVALYAADATAFPD
                     GTPLTTAGLRDALVYGPASDPDNGLLALLAAGQPQIDENSRGEATAHALQRCPNGSGG
                     PRHSDGYRAATPSPGTANTCPLDAPPAISAVSPAAGATAVSLDTTLLITFTEPVTFSG
                     RWFALSCDGHEQTVTTLPGETRATVVPGPALPAGATCVAVVSAGGIADQDPDDPPDTM
                     TADTIWSFATLPAPPPLVARFHSNSPVWIATPVLFTNTTTGPGTPAFTWDFGDGHGST
                     LAHPRHTYAAPGT"
BASE COUNT          442 a          898 c         1138 g          512 t
ORIGIN      
        1 aggtgccggg ggcggcgtag gtgtggcggg ggtgggccag ggtggagccg tggccgtcgc
       61 cgaagtccca cgtgaacgcg ggcgtgcccg ggccggtggt ggtgttggtg aagaggacgg
      121 gggtagcgat ccacacgggg ctgttgctgt ggaagcgggc cacgaggggc ggcggggcgg
      181 gcagcgttgc gaatgaccag atggtgtcgg cggtcatcgt gtcgggcgga tcgtccgggt
      241 cttgatcggc gatgcctccg gcagagacga cggccacaca ggtcgcgccg gctggcaggg
      301 cggggccggg cacgaccgtg gcccgtgttt cgcccggcag ggtggtcact gtctgctcgt
      361 ggccgtcgca ggagagggcg aaccagcggc cgctgaaggt cactggctcg gtgaaggtga
      421 tgaggagggt ggtgtccagg gagacagctg tcgcgcccgc ggccggagac acggcgctga
      481 tggccggtgg ggcgtcgagc gggcaggtgt tggccgtgcc ggggctgggg gtggcggcgc
      541 ggtagccgtc actatggcgc gggccgccgc tgccgttggg gcaacgttgg agggcgtggg
      601 cggtcgcctc gccgcggctg ttttcgtcta tctgtggctg cccggcggcc agtagggcca
      661 gcaggccgtt gtcggggtcg ctggcggggc catagacgag ggcatcgcgc aggccggcgg
      721 tggtgagtgg cgtgccgtcg gggaaggcgg tcgcgtcggc ggcgtagagg gcgacggcgt
      781 ctggcccatt ttgcaacgtg gcgtcggcca acggccagtc gacccccggt agcgtggcgc
      841 tgccaagcac gaaatagcct tccgcgtcgg tctgctgccc ggccagatcg atggcctggt
      901 aggcagtgtc gtcttgcccg ttccagagga cgacgacgag gccgctgagg tcggtatcgc
      961 cgcgtccgcc gtcatagagt tcaatgaact cagccgtgtc ctggccgggc gtgtcactat
     1021 ccacttcgtt catgaggatg ccatccgtgg cgaccggggc gatgcggaag tgccaggtgt
     1081 agttgtctgg caagtggtcg ggcggatcgt cggggtcggc gtcgtggacg gcggtggccc
     1141 cgagagtgac ggtacactcc tccgcgtccg cgaaaggcag cgccggcgtg agcgtaaaag
     1201 tcatcgggcc gccacctgtg tccgccgcgt ggctgccgct gacgctacag gtgatcgtga
     1261 accagttgct atcgagcgcg accggttcgc taaattgcac acgcagttgt gccagtgcgg
     1321 acacgtgttc ggccccattg gcgggcgaga cggcctcaat gactggcggc gcatcgacac
     1381 ggcagttgtt gggcgcgtta ggcgtgggtg tgttctggag ataggcaatg gtttggcgtg
     1441 gtaagccagc gccatcgggg caccggccgc tggagtcgct ctcagccgcg gcgcgggcat
     1501 tctcgtccac ctgggcctgt cccgcagcca gcagaaccag aaggccgttg tccgttggat
     1561 cggacgtgcc gtagacgatg gcgtcgcgga ggccggccgt ggtaatggcc gtgccgctgg
     1621 ggaagtcggc ggcgtcgccc tcatagagcg cgaccgcgtc ggggccgttc tgaataacgg
     1681 ccgcgggtag cgttgctact cctcccagcg ccgggccgcc gatgatcgcg tagccgtcgc
     1741 cgttcgtgcg cgcgccatcc aaatcgacgg cgaagtaggc gcggtcgtcc ttgccattga
     1801 acaacaccag caccagcccg gatagatcag ttccaccagt tccaccgtcg tagagttcga
     1861 cgaattcggc tgtgtcgctg ccagacgtgt cgctgtcgag ttcattgatc aggatgccgc
     1921 cggccggggc gggagcaacg cggaagcgcc aggtgtagtc ggccgccagc gtgtcgggcg
     1981 gatcgtcgct gtcggcatca tgtacggccg ccgcgcggag ggtggtggcg cattgctcgc
     2041 cgtggctgaa gggcaacgtt ggcgtcagga tgaaagttgt ggggccaccg gccgacgcgg
     2101 ccgcgtggct gccgctgaca tcgcagacaa tgctgaacca gttgttgtcc agcgtcaccg
     2161 cttcactgaa taccacttct aattgggttt gcgcggtcac accgtcagca ccgtcggccg
     2221 gcgagaccgt ggcaacggcc ggcgggacgt cggcgtggca gtcgttcgct ctccccggcg
     2281 tcggcgcgtt ctggcgatag gtcgccgtat agcgccgtcc gccggcccca tcgggacagc
     2341 gaccgctgga atcggcttcg gccgcggcgc gggcgttttc atcgacctgg gcctgtcccg
     2401 cagccagcag aaccagaagg ccggtgtccg ttggatcgga cgtgccatag acgatggcgt
     2461 cgcgcaggcc ggcggtcgtc agggccgtgc cgttggggaa gtcggcggcg tcaccctcgt
     2521 agagcgcgat cgcgtcgggg ccgttctgga tcatggccgc gggcagcgtt agagcacccc
     2581 ccaacgcagt gctgccggca acggcgtagc cgtcctcatc cgtccgtaca ccgtccaaat
     2641 cggcagcgaa gtaggcacgg tcgtccgtgc cattgaaaaa gaccagcacc agcccggaca
     2701 gatcagtctc accggcgcca ccgtcgtaga gttcgacaaa ctcggccgtg tcgctgccgg
     2761 gcgtgtcgct atccagttca ttgatcagga tgccgccggc cggtgcgggg acaatgcgga
     2821 agcgccaggt gtagtcggcc accagcgtgt cgggcggatc gtcgctgtcg gcgtcgtgga
     2881 tagctgttgc tcgaaggctg accacgcact gctcgttgtg gctgaagggc aaggccgacg
     2941 ccagtggtag ccacacagcc acaaccgggg gtggcccgac caccttcact
//
LOCUS       DAOSMQ010000402         2959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_33462_length_2959_cov_3.649449, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000402 DAOSMQ010000000
VERSION     DAOSMQ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33462_length_2959_cov_3.649449"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PK829_11890"
     CDS             <1..802
                     /locus_tag="PK829_11890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU41959.1"
                     /translation="TATPPSPMTAMNLSYDAIARYYDLSHQSLVEDIPFLLRVAAEAG
                     GPALELGCGSGRLLVPLARAGYAVTGVDNSPEMLARAELRLAGEPAEVRDRVRLVPAD
                     VRALALPAAAPFGLAYFGYNTFMHLDETSAATTLRRVRPLLRPGARLLIDVDHPLALS
                     TAADDPDFILEDVLRDNALGETIRQYTAYESVPGEQAVDVSWVYESDRGARTERTRVK
                     LRYHYFYPHQFDILFGLTGFRLLALHGSYAGTPFAESSERLIVEGEAS"
     gene            complement(968..2632)
                     /locus_tag="PK829_11895"
     CDS             complement(968..2632)
                     /locus_tag="PK829_11895"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HOU41960.1"
                     /translation="MRTLEQALLDHDLIVLRVIAEWLELDLTGADKAQSVKQLAAALM
                     AVDLAEEIEGLEPEEAGALAALVREGGRAPVAVFSRAHGEVRAMGPGRLEREEPWLDP
                     ISPAEALWYRGFLYRGFDQTSEGLLEFFYLPQELLARLQPSPAAAPREMAAAPTLTPI
                     DPPEGTRPPVDDAVDDLTTLLALAQRTGLQPDRLPDLRGLLMNPDRDRRSLLLTLATE
                     MSLLRRAEEWLRPTRAAVDWLQHSREAQLRALVDAWSRSVWNELRHTPGLVAEGESWA
                     NDPLLARTALLDVLPADDHWYRVADVVAIVRRQDPDFQRPDGNYDTWYLRDAETREYL
                     SGFETWERVEGRLLPFLIQGPLYWLGMAELSATTDPALAAYRLAPRALAWLGDEPPPA
                     DEVRVPLVVQPEGVLLVPHNASRLDRFRAARIADPEPFVPGRPYRYRIVPSSLAWARE
                     QDISPEQMLGFLESASGRPVPPSVRRGITRWAERGVEGRLQSVVVLRVGDPAILETLR
                     TNPKTRDFLTEALGELAVVVRAGQWEAFRQAVAALGLLLDVEAGEP"
     gene            complement(2644..>2959)
                     /locus_tag="PK829_11900"
     CDS             complement(2644..>2959)
                     /locus_tag="PK829_11900"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41961.1"
                     /translation="YAGVGPRGGGGFLMTPDARWDDDRIDTCLCNPVGRLTMLAMLTR
                     VMKGTHVTSRHVTMRRNHTITARSSSPMPIYVDGEMFAYPRDGVHEVTITSVPAAVEV
                     MV"
BASE COUNT          464 a         1027 c         1010 g          458 t
ORIGIN      
        1 aaccgcgacg ccgcctagcc cgatgactgc catgaacctc agctacgacg ccattgcccg
       61 ctactacgac ctcagccacc agtccctggt cgaagatatc ccttttctgc tgcgcgtcgc
      121 ggccgaggcg ggcggcccgg cgctggaatt gggctgcggc agcgggcgct tgctcgtgcc
      181 cctggcccgc gcgggctacg ccgtcaccgg ggtggacaac tcgccggaga tgctggcgcg
      241 ggcggagttg cggctggcgg gcgagccggc agaggtgcgc gaccgtgtgc ggctggtgcc
      301 ggccgacgtg cgcgcgctgg ctctgccggc ggcggcgccg ttcgggctgg cctacttcgg
      361 ctacaacacc ttcatgcacc tggacgagac cagcgcggca acaaccctgc ggcgggtgcg
      421 gccactgctg cggcccggcg cgcggctgct catcgacgtg gatcacccgc tggcgctatc
      481 gaccgcggcc gatgaccctg acttcatcct cgaagacgtg ctgcgcgaca acgcgcttgg
      541 cgaaaccatc cgccagtata ccgcctatga gtccgttccc ggcgagcagg ccgtcgatgt
      601 tagctgggtc tacgaatcag accgcggggc cagaacagag cgcacacgcg tgaagctgcg
      661 ctaccactac ttctaccccc accagttcga tatcctcttc ggcctgaccg gcttccggct
      721 gttggccctg catggcagct acgccggcac gccctttgcc gagagcagcg agcggctgat
      781 tgtggagggg gaagcgagtt agtgaccagt aggtcagtgt ccagtggtca gtagggcagt
      841 gtccagtggt cagtagggca gtgtccagtg gtcagtaggg cagtggtcat cggcagaagt
      901 taacgcaagg acgcgaagtt ctagaccgcg ccctttttct cccctgctcc cctgctcccc
      961 cgcattctta cggctccccc gcctccacat ccagcagcag gcccaacgcg gcgacggcct
     1021 ggcggaacgc ctcccactgc ccggcgcgca cgacgacggc cagctcgccc aatgcctcgg
     1081 tcaggaagtc gcgcgtcttg gggttggtgc gcagcgtctc caggatggcc gggtcgccca
     1141 cgcgcaagac gacgaccgat tgtagccgtc cctccacgcc gcgctctgcc caacgcgtga
     1201 tgccgcggcg gacgctgggc ggcacgggcc ggccactggc cgattccagg aagccgagca
     1261 tctgctccgg gctaatatcc tgttcgcgcg cccaggccaa cgacgagggt acgatgcggt
     1321 agcgataggg ccggccgggc acaaacggct ccgggtcggc gatccgcgcc gcgcggaagc
     1381 gatcgaggcg actggcgttg tggggcacca gcagcacgcc ttccggctgg acgaccagag
     1441 gcacgcgcac ctcgtcggcc ggcggcggct cgtcgcccag ccaggccagc gcccgcggag
     1501 ccagccggta ggctgccagc gccgggtcgg tcgtcgccga tagctcggcc atgcccagcc
     1561 agtagagcgg cccctgaatg agaaagggca gcagccgccc ctcgacgcgc tcccacgtct
     1621 cgaaaccgct caggtattcg cgcgtctcgg cgtcgcgcag gtaccaggtg tcatagttgc
     1681 catccggccg ctggaagtcg gggtcctggc ggcgaacgat ggcgacaaca tcggccacgc
     1741 ggtaccaatg gtcatcggcc ggcaacacgt ccagcagcgc cgtccgggcc agcagcgggt
     1801 cgttggccca actctccccc tcagccacca gccctggtgt gtggcgcagc tcattccaga
     1861 cgctgcggct ccaggcatcg accagggcgc gcaactgggc ctcgcggctg tgctggagcc
     1921 agtcgacggc cgcgcgcgtt ggccgcagcc actcctccgc ccgccgcagg agcgacatct
     1981 ccgtggccag cgtcagcaac agcgagcgcc ggtcgcggtc gggattcatc agcaggccgc
     2041 gcaggtcggg caggcggtcg ggttgcaaac cggtgcgctg ggccagagcc agcagggttg
     2101 tcaggtcgtc cacggcgtca tcgacgggcg ggcgggtgcc ttccggcggg tcgatgggcg
     2161 tcagggttgg cgcggcggcc atctcgcgcg gcgcggcggc cggtgacggc tggagcctgg
     2221 ccagcaactc ctggggcagg tagaagaatt ccagcagccc ctcgctcgtc tggtcgaagc
     2281 cgcggtagag gaagccgcga taccacagcg cctcggccgg gctgatgggg tcgagccacg
     2341 gctcctcgcg ctccaatcgg cccggcccca tggcccgcac ctcgccgtgg gcacggctga
     2401 agacggccac gggggcgcgt ccgccctcgc gcaccagcgc ggccaacgcc ccggcctctt
     2461 ccggctccag accctcgatc tcttcggcca ggtctacggc catcagcgcc gcggccagct
     2521 gcttgaccga ctgcgccttg tcggcccccg tcaggtcaag ctccagccat tcggcaatga
     2581 cacgtagaac gatgaggtcg tgatccagca atgcctgttc aagcgttcgc atagcttccc
     2641 ctgttagacc atcacttcga ccgcggcggg cacactggta atggtgactt cgtggacgcc
     2701 atcgcgtgga taggcgaaca tctcgccgtc tacgtagatg ggcatcggcg acgacgaccg
     2761 ggcggtgatt gtgtggttgc ggcgcatggt tacgtggcgg gaggtgacgt gcgtgccttt
     2821 catgacgcgg gtcaacatgg ccagcatggt cagccggccg accggattgc atagacaggt
     2881 gtcgatgcgg tcgtcgtccc aacgagcatc gggggtcatc aggaagccgc cgccgccacg
     2941 cgggccaacg ccggcatag
//
LOCUS       DAOSMQ010000403         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_33487_length_2957_cov_4.534459, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000403 DAOSMQ010000000
VERSION     DAOSMQ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33487_length_2957_cov_4.534459"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..146)
                     /locus_tag="PK829_11905"
     tRNA            complement(57..146)
                     /locus_tag="PK829_11905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Ser,seq:gga)
     gene            complement(284..1897)
                     /gene="ggt"
                     /locus_tag="PK829_11910"
     CDS             complement(284..1897)
                     /gene="ggt"
                     /locus_tag="PK829_11910"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU41962.1"
                     /translation="MALTFSDMHFPSRRAPVVAGNGLVATSQPLAAQAGLAVLQAGGN
                     AIDAAVATVATLSVVEPCSTGIGGDAFALIWSAAEGRLFGLNASGPAPAGLTADLVRG
                     RGHTTFPARGGLPVTVPGALRGWQLALERFGTRGLDTLLARPIDYAANGYPVSPGIAA
                     AWERSTELLKRLPDSRRVWLPGGRAPRATERFRNPEFAATLRTIAEQGYDAFYTGSMA
                     ERVAAAVRADGGVMSAGDLSAYRAEWVEPIAVPYGDGYVFHEIPPNGQGLAALLALNI
                     ARGFSLKDMTYGSADYYHVLIEAMKLAFADAHAYIGDPRRVDVPVGGLLSQRYARERR
                     ALIAKDHALFPQPGRPPRDGDTVYLTVADAAGNMVSWIQSLYMGFGSGITAADTGVQL
                     QNRGANFSLERGHPNEVAPGRRPYHTIIPGFITRDGRPWASFGVMGGFMQPQGHLQVG
                     VNLVDFGMDPQAALDAPRFNWLEGREVALEPGIGAETRDALAHRGHALWPLGAAVHYG
                     GGQVILRDNDSGVLIGGAEPRNDGAAVGY"
     gene            complement(1897..2307)
                     /locus_tag="PK829_11915"
     CDS             complement(1897..2307)
                     /locus_tag="PK829_11915"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HOU41963.1"
                     /translation="MPRLYLRASCHGNPGPGTWAVALEQDSEWETSAGSDPRTTNNRM
                     ELRAAIEGLLLLPPGSPAQLFTTSDYLYQGATRWLANWKRGGWQKKDGQPVANGDLWQ
                     LLDDLSRHYQVHWVNAKGQRLEGLTRAGQALTSA"
     gene            complement(2350..2763)
                     /gene="rnhA"
                     /locus_tag="PK829_11920"
                     /pseudo
     CDS             complement(2350..2763)
                     /gene="rnhA"
                     /locus_tag="PK829_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
BASE COUNT          446 a         1031 c         1013 g          467 t
ORIGIN      
        1 gagaaagcaa ttggcgagcc tgttggccag gctcgccaat gagttcgtcc ttcaattggc
       61 ggagagggcg ggattcgaac ccgcgacccc ttgcgaggta cacgatttcc aatcgtgcgc
      121 actagaccgg actatgcgac ctctccaaat cgacggccat tatatccgct cgctggccct
      181 ctgcccaatc aagtcagggc ttttccaaag gtacccaggc gcaccgacct gacaggtggc
      241 cgcccaccgg tcaggtcctg agtcatcagc gtgcccccga cgttcagtaa cccacggctg
      301 cgccatcgtt gcgcggctca gcgccgccga tgaggacgcc gctatcgttg tcgcgcagaa
      361 tcacctgccc gccgccatag tggacggccg cgcccagagg ccagagcgcg tgcccgcggt
      421 gggccaacgc gtcgcgcgtc tccgcgccaa tgcccggttc cagcgccact tcgcgcccct
      481 ctagccagtt aaagcgcggc gcgtcgaggg ccgcttgcgg gtccatgccg aaatcgacga
      541 gattgacgcc cacctgcaag tgcccctgcg gctgcatgaa gccacccatg acgccgaagc
      601 tggcccacgg ccggccgtcg cgggtgatga agccggggat gatggtgtga tagggccggc
      661 ggccgggagc cacctcgttg gggtggccgc gctccagact aaaattcgcg ccgcggttct
      721 ggagttgcac gcccgtgtcc gccgcggtaa tgccgctgcc aaagcccata tagaggctct
      781 gaatccagct gaccatgtta ccggccgcgt cggccacagt cagatagacc gtatcgccgt
      841 cgcggggcgg gcggcccggc tgcggaaaca gggcgtggtc tttggcaatt agcgcgcggc
      901 gttcgcgggc gtagcgctgg ctcagtaagc caccaacggg cacatcgacg cggcgtgggt
      961 cgccgatgta ggcgtgggcg tcggcgaaag ccagcttcat ggcctcgatg aggacgtggt
     1021 agtagtcggc cgagccgtag gtcatgtctt tcaggctaaa gccgcgggcg atgttgagcg
     1081 ccaacagagc cgccagcccc tgcccattgg gcggaatctc gtggaagacg tagccgtcgc
     1141 catagggtac ggcgatcggc tcgacccact cggcccggta ggcggatagg tcgccggcgc
     1201 tcatgacccc gccgtcggcg cgcacggccg cggcaacgcg ctcggccata gagccggtgt
     1261 agaaggcatc gtagccctgc tcggcaatcg tgcgtaatgt ggcggcgaac tccgggttgc
     1321 gaaaccgctc cgtcgcccgc ggcgcgcgcc cgccgggcag ccagacgcgc cgcgaatcgg
     1381 gcaagcgctt tagcagttcc gttgagcgct cccaggcggc ggcgatgccg ggactgaccg
     1441 ggtagccatt ggcggcatag tcgatgggcc gggccagtag cgtgtccagc ccgcgcgtgc
     1501 cgaagcgctc cagcgccagt tgccagccgc gcaacgcgcc gggcaccgtc acgggcaggc
     1561 caccgcgggc cgggaaggtc gtgtggcccc ggccgcgcac caggtcggcc gtcaggccgg
     1621 ccggggctgg gccgctggcg ttcagcccga acaaccgtcc ctcggccgcc gaccagatca
     1681 gggcgaaggc gtcgccaccg atgccggtcg agcacggttc gacgacactc agcgtcgcca
     1741 cagtggccac ggcggcgtca atggcgttgc cgccggcttg cagcaccgcc agtccggcct
     1801 gcgccgccag cggctggctt gtggccacca ggccattgcc agcgaccaca ggcgcgcgcc
     1861 gcgaggggaa atgcatgtcg ctaaaggtta gagccattat gcgctcgtca gggcctgtcc
     1921 cgctcgcgtc agcccctcca gccgctgccc cttggcgttc acccagtgaa cctgatagtg
     1981 ccgcgagagg tcgtccagta gttgccacag atcgccgttg gccaccggct ggccatcctt
     2041 cttctgccag ccgccgcgct tccagttcgc cagccagcga gtagccccct ggtagaggta
     2101 gtcggacgtg gtgaagagtt gcgccgggct gccgggcggc aacagcagca acccttcgat
     2161 ggcggcgcgc aactccatgc ggttgttggt cgtccgcggg tcactgccgg cgctcgtctc
     2221 ccactcgctg tcctgctcca gggccacagc ccacgtgccc gggccggggt tgccgtggca
     2281 ggaggcgcgc agatagaggc gcggcacgcc agcgtcgagg tcgatgcgcg gggtgatggc
     2341 cagccgggcc tcgcgggcca gttggtcgac ccgctcattg tccggctcgc cactgtgccc
     2401 ccgcacccag tgccagacga tctcgtggcg ggccgtctgc ccctgcagcg cgcgccacag
     2461 gtcggcgttg gggatggcct ggccgttctt cttcacccag ccggcggcgc tccacttggc
     2521 cacgccctcg gtgatgcccc ggcgcacgta ctgcgagtcg gtgtggaacg acacgcggca
     2581 cggccgcttg agcgccgcca gggcggcgat ggctgcctgt agctccatgc ggttattggt
     2641 cgtcttggga tcgctgccgg tcagcacccg ctcgcgctcg ccggcgcgca ggatggccgc
     2701 ccagccgccg atgccggggt tggggtcggc cccgccgtcg ctatagatcg tgacttcgtc
     2761 catcggccgt cccgtctcag cgacgacgcg ggcacgcggt cggtcggggc gtcactgggc
     2821 caatgccgcc agcatgtcgt ccacgcgggt gaacttgacc cgcggccggc cctgctcctt
     2881 gcctcggctc agttccagcg catcgagccg ctgccaatcg gcaaaggtga cgacgtgcgg
     2941 ctgccggcta cggacca
//
LOCUS       DAOSMQ010000404         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_33551_length_2952_cov_3.777011, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000404 DAOSMQ010000000
VERSION     DAOSMQ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33551_length_2952_cov_3.777011"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PK829_11925"
     CDS             complement(<1..669)
                     /locus_tag="PK829_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU41964.1"
                     /translation="MSLDKIIVRGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLGQMEKPDVDNIEGLSPAVSIDQRGVSHNPRSTVGT
                     VTEIYDYLRLLFARVGTPHCPVCGRPVMPQSAEQIVGNVLAMPTGTRIQVLAPLVQDR
                     KGHHQTVFDDVRKAGFVRVRVHGEVRQVDDKIELDRYKNHTIEAVVDRLVIRHAADDT
                     SEEAA"
     gene            1063..1467
                     /locus_tag="PK829_11930"
     CDS             1063..1467
                     /locus_tag="PK829_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU41965.1"
                     /translation="MANKRRVGIIAGVVAVLVIVGGYLVYSNGQTAVAQEDTTEVETS
                     IVRTGSITVSATGAGTVIPARQVDLAFSTSGVLTELAVGVGDQVAAGDELARVNDTAA
                     QHALATAQLPSSAASTAPAKGATFWPGKTSAV"
     gene            1398..1721
                     /locus_tag="PK829_11935"
                     /pseudo
     CDS             1398..1721
                     /locus_tag="PK829_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1889..2683
                     /locus_tag="PK829_11940"
     CDS             1889..2683
                     /locus_tag="PK829_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41966.1"
                     /translation="MANNDTTATVELSALVQQIQRAMTAEQVQAIADMKLTAGSLTAL
                     QASGALAFGGPGSTDGATSGDGQTRPDFSNFQPPADGSGGFAGGGPGGGAATGGGQGG
                     AFAGGPPAGSDFSGGALSEDDIATRQAAMANMDPAEMQSRMLTGMVVRLLQTKTGQTP
                     TGPGGPGGGAFDAAVTAVAEATGLSADDIRAQMNDGQTLTGIVAANGGDMTAVRAAVV
                     AALQALPEAANMDVEAMADRLLAQSMPSGQGAPPAAQATPAAAATP"
BASE COUNT          503 a          986 c         1012 g          451 t
ORIGIN      
        1 ggcggcctct tcgctggtgt cgtcggcggc gtggcggatg accaggcggt cgacgacagc
       61 ctcaatggta tggttcttat agcggtctag ctcgattttg tcgtccacct ggcgcacttc
      121 accgtgaaca cgcacgcgga cgaaaccggc cttgcgcacg tcgtcgaaga cagtctggtg
      181 gtggcccttg cggtcttgca ccagcggggc cagcacctgg atgcgcgtac ccgtgggcat
      241 ggccaggacg ttgccgacga tttgctcggc cgactggggc atgaccggcc ggccgcagac
      301 gggacaatgg ggcgtgccca cgcgggcaaa gagcagacgc aggtagtcgt agatctcggt
      361 gaccgtgccc accgtcgagc gcgggttgtg gctgacgcca cgctggtcga tggagacggc
      421 cgggctgagg ccctcgatgt tatcgacgtc aggcttctcc atctgaccga ggaactgccg
      481 ggcataggcc gacagcgatt cgacgtagcg gcgctgccct tcggcaaaga tcgtgtcgaa
      541 ggccagcgac gacttgcccg aaccggacag gccagtgatg accaccagct tgtcgcgggg
      601 aatttctacg tcgatgttct tgagattgtg ttcgcgcgcg ccgcgcacaa tgattttatc
      661 gagcgacata cttccgtttc ctgctgcgcg aagaataagc ccggccacca tctccgcgcg
      721 accgacgcgc cgcgcgtcga ccccaataga cgactcgcta ttctagcata gcgcccgccg
      781 gcgtaccatt agcgtttggt tggatgggcg agacgggcac gcaatgggtg agtatagcac
      841 agatggtcta gtctcacaag gggcagcaga cgcgattcgg ggcttctcta gagtcgtcta
      901 gcgaccattg cctggggcga ctttggaaaa gccctgagac gcgattcggg agaatcgcgc
      961 cacgggtgtg cattttacca gccggcaaca gcttcttcac gagtcctgaa caattgcggc
     1021 ctacactgac gagtgcgaga accactcgtg gaggcatgca tcatggcaaa taagcgtaga
     1081 gtagggatca tcgccggcgt tgtggccgtg ctggtcattg tcggcggcta tttggtctac
     1141 agcaacggcc agacggccgt ggcccaggag gacaccaccg aggtggagac ctcgatcgtg
     1201 cgtactggca gcatcaccgt ctcggccact ggcgcgggca cagtcatccc cgcccggcag
     1261 gtcgatctgg cgttctcgac cagcggcgtg ttgaccgaac tggccgtcgg cgtcggcgac
     1321 caggtggcgg cgggcgacga actggcccgc gtcaacgaca cggcggccca gcacgccctg
     1381 gcgacggccc aactgccatc ctcggctgcc tcgaccgccc cagcgaaggg cgctactttc
     1441 tggccgggca agacgtcagc ggtctgagca acaagcagct tgcagccatt cgcaaccggc
     1501 agatcggctt cgtcttcccg tcgttcaacc tgttgtcacg caccagcgcc gtcggcaacg
     1561 tcatgctgcc gctggtctac aagcgcggcg cgggcacgcc accgtcggcc gagcgccatg
     1621 agcgggccat ggccgcgttg gaggccgtcg ggctggccga ccgggccaac cacatgccca
     1681 acgagatgtc cggcggccaa cgccagcgcg tgaccatcgc cgacggccgc ttcttcaacg
     1741 agaaggacat ggcccagaca gccaaggtgg cgatcctggg cgcgggcgtg gccgccgacc
     1801 tgttcggcga cgccgacccc attggccctg gccgcctgca cccccaccgg cggccacgct
     1861 cctgccactg tggcaggcgt atcagtcgtt ggccaacaac gacacgacgg cgacggttga
     1921 gctgagcgcg ctggtgcagc agattcagcg ggccatgacg gccgagcagg tgcaggccat
     1981 tgccgatatg aagctgaccg ccggcagcct gaccgcgttg caggcgagcg gcgcgctggc
     2041 gttcggcggc ccgggcagca ccgacggcgc gacctccggc gacgggcaaa cgcggcccga
     2101 cttctccaac ttccagccgc cggcggatgg cagcggtggc tttgccggcg gcgggccggg
     2161 cgggggcgca gcgacaggcg gcggacaggg cggcgccttc gccggtgggc cgccggcggg
     2221 cagcgacttc agcggcggcg ccctgagcga ggacgacatc gccacgcgcc aggcggcgat
     2281 ggcgaacatg gacccggccg agatgcagag ccggatgctg accggcatgg tcgtccgcct
     2341 gctgcaaacc aagacgggcc aaacgcccac tgggccgggc gggccgggcg gcggggcatt
     2401 tgacgctgcg gtgacggctg tggccgaggc caccggcctg agcgccgacg acatccgcgc
     2461 ccagatgaac gatggccaga cgctgaccgg catcgtcgcg gccaacggcg gcgacatgac
     2521 cgccgttcgc gccgccgtcg tcgcggcgct gcaagcgctg cccgaggcgg ccaacatgga
     2581 cgtggaggcg atggccgacc ggctgttggc gcagagcatg ccgtcggggc agggcgcgcc
     2641 gccagcggct caggcaacgc cagccgcggc agccacgccg taaagtgagc cggggcggcc
     2701 gcgcggctaa aactcgcccg gtcttcttgt ctattcgtcc accgggggcg agccgcgcgg
     2761 cccgccccct tcagaggcaa ccgcggcggt gccaatcccc aggcctcacg tttatcgcgc
     2821 cgcttacccc tcccagtcac cgctacagac ccgcccgtcg atcccagggt cgggtcgtct
     2881 ttccaggcgc aactatgagc gtgaaatgag cggccgtctg cccgccgatg cagccccccg
     2941 aggcaagcgt at
//
LOCUS       DAOSMQ010000405         2949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_33589_length_2949_cov_4.896683, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000405 DAOSMQ010000000
VERSION     DAOSMQ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33589_length_2949_cov_4.896683"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK829_11945"
     CDS             complement(<1..383)
                     /locus_tag="PK829_11945"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU41967.1"
                     /translation="MITIPVQHDTDDADFRQLSEFVVAQMREHQVPGVAVGLWHDGRE
                     RLAGYGITSVENPLPVTADTLFQIGSTTKTITGTIVMRLVEQGLIDLDRPVRDYLPEL
                     RLRDKTAEATVTMRHLLTHMGGWLG"
     gene            complement(401..2923)
                     /locus_tag="PK829_11950"
     CDS             complement(401..2923)
                     /locus_tag="PK829_11950"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOU41968.1"
                     /translation="MNRAVRILLIIVGVLAVLALVLFAGGMAVARSPFPDTDGEKALE
                     LPAACETAGEGEEAALCVALTGHGLTAPVHIYRDEHGIANIYAENAADLFFAQGYVHA
                     QDRMWQMEFWRHIAMGRVSEIVGEPGLENDKFIRTSGWNRIAAANLAYYEQELPEAMA
                     ALEAYSAGVNAYLVENKDDIAISQRILGLVGEPWTIEPWEPLHSIGWGVVMGWDLGGN
                     WDDELTRARLYAAIGQEATDELVPGYPYDTRPVIAPTGLLVSAANTTSGASPAVAAVD
                     WTQVSTQLIGQPPAALGDGPFLGSNNWVVSGEHTASGLPLLANDPHLGIQMPSIWYQV
                     GLHAPGWDVTGFSFAGIPGVIIGHNDRIAWGVTNVGPDVQDLYIEKINPDNPNQYEYM
                     GEWRDMNLIEEVIKVNGGEDVTLTVRETQHGPIISDVLEDQPDVLAMRWTANNGPSRI
                     LQSALLLNQAQNIDDFREALRYWDIPSQNFVYADVDGNIAYQTPSLIPIRANGQGMVP
                     VPGWTGEYEWQGYIPYEELPALLNPEQGYIVTANNAVVDEQYPYFIARDWASGDRAQR
                     ITAMIDAAIADGPIDADDFARIHFDSYSTRAESYVPLLTGLASDDAEVQRALALLRDW
                     DYQERRDSVATSIFEMFYMHLVRNVLSDDLGEENYLDGHSTIYFHQLANLPDARWWDD
                     VTTPKVETREDILLRSLSDGLAWLHANLGDDMDGWTWGRLHTATFSSTPLGESGIGPI
                     ESLVNRGPFPADGGTDLVNATSWRFTEPAVITSHPSMRMIVDLSDFDASRWVIPTGQS
                     GHPFNAHYDDQIELWLNGEYLPMLSSREAIEAAATDQLVLQP"
BASE COUNT          453 a          897 c         1053 g          546 t
ORIGIN      
        1 tcgcccagcc agccgcccat gtgggtcagc aagtggcgca tggtgacagt ggcctcggcc
       61 gtcttgtcgc gcaggcgcaa ctccggtaga tagtcgcgca ccggccggtc gaggtcgatc
      121 agcccttgct ccaccaggcg catgacgatt gtgccggtga tggtcttggt cgtcgagccg
      181 atctggaaga gcgtgtcggc cgtgacgggc agggggttct cgacgctggt gatgccatag
      241 ccggccaggc gctcgcggcc gtcgtgccac aggcccacgg ccacgccggg aacctggtgc
      301 tcccgcatct gggcgacgac gaactcactc aactgccgga aatcggcgtc gtcggtgtcg
      361 tgttgcacag gaatggtgat catcgggttt tgccttgcgc tcagggttgc agcaccaact
      421 ggtcggtcgc ggcggcctcg atggcctcgc ggctggacag catgggcagg tattcgccat
      481 tgagccacag ctcgatctgg tcgtcgtagt gggcgttaaa cgggtggccg ctctggccgg
      541 tggggatgac ccagcggctg gcgtcgaagt cgctcaggtc aacgatcatg cgcatggacg
      601 ggtggctagt gatgaccgcc ggttcggtga agcgccaact cgtggcgttg accaggtcgg
      661 tgccgccgtc ggccgggaag gggccgcggt tgaccagcga ctcgatgggg ccaatgccgc
      721 tctcgcccag gggtgtgctg ctgaaggtgg ccgtgtgcag ccggccccac gtccagccgt
      781 ccatgtcgtc gcccaggttg gcgtgcagcc aggccaggcc gtcgctcaac gagcgtagta
      841 agatgtcctc gcgcgtttcc accttcggcg tcgtcacgtc gtcccaccag cgggcatcgg
      901 gcaggttggc cagttggtgg aagtagatcg tgctgtggcc gtcgaggtaa ttttcctcgc
      961 ccaggtcatc gctgaggacg ttgcgaacca ggtgcatgta gaacatctca aagatgctcg
     1021 tggccacgct gtcgcgccgc tcctgatagt cccaatcgcg cagcaacgcc agtgcccgct
     1081 gcacctcagc gtcgtcggag gcgagaccgg tcagcagggg cacgtagctc tcggcgcgcg
     1141 tggagtagct gtcgaagtgg atgcgagcga agtcgtcggc gtcgatgggg ccgtcggcga
     1201 tggctgcgtc gatcatggcg gtgatacgct gggcgcggtc gccgctggcc caatcgcggg
     1261 cgatgaagta ggggtactgc tcatcgacca cggcattgtt ggccgtgacg atatagccct
     1321 gctccgggtt aaggagggcg ggcagttctt cgtaggggat gtagccctgc cactcatact
     1381 cgcccgtcca gccgggcacg ggcaccatgc cctggccgtt ggcgcggatg gggatgaggc
     1441 taggcgtctg gtaggcgatg ttgccatcca cgtcggcata gacgaagttt tgcgacggga
     1501 tgtcccaata gcgcagggct tcgcggaagt catcgatgtt ctgggcctgg ttgagcaaca
     1561 gcgccgactg caagatgcgc gacgggccgt tgttggccgt ccagcgcatc gccaggacat
     1621 cgggctggtc ttcgagcacg tcgctgatga tcggcccgtg ctgggtctcg cgcacggtca
     1681 gggtgacatc ctcaccgccg ttgaccttga tgacttcctc gatgaggttc atgtcgcgcc
     1741 actcgcccat gtactcgtac tggttggggt tgtcggggtt gatcttctcg atgtagaggt
     1801 cttgcacgtc cgggccgacg ttggtgacgc cccaggcaat gcggtcgttg tggccgatga
     1861 tgacgccggg gatgccggcg aagctgaagc cggtcacgtc ccagccgggc gcgtgcaggc
     1921 cgacctggta ccagatcgac ggcatctgga tgcccaggtg gggatcgttg gccagcaggg
     1981 gcaggccgga ggcggtgtgc tcgccggaga cgacccagtt gttgctgccc aggaaggggc
     2041 cgtcgcccag cgccgccggc ggttggccaa tgagttgcgt ggagacctgc gtccaatcga
     2101 ccgcagcgac agccggcgac gcgccgctgg tcgtgttcgc ggccgagacg agcaggcccg
     2161 tcggggcgat gaccgggcgg gtgtcatagg ggtagccggg cacgagttcg tccgtggctt
     2221 cctggccaat ggcggcgtag agccgggcgc gggttagctc gtcgtcccag ttgccgccca
     2281 ggtcccagcc catgaccaca ccccagccga tggagtggag cggctcccac ggctcaatcg
     2341 tccacggctc gccgacgagg ccgagaatgc gctggctgat ggcgatgtcg tccttgttct
     2401 cgaccagata ggcgttgacg ccggcgctgt atgcttccag cgcggccatc gcctccggca
     2461 attcctgctc ataataggcc aggttggccg cggcgatgcg gttccagccg ctggtgcgga
     2521 tgaacttgtc gttctccagc cccggctcgc cgacgatctc cgagacgcgc cccatggcga
     2581 tgtgacgcca gaactccatc tgccacatgc gatcctgggc atgaacgtag ccctgggcga
     2641 aaaagaggtc ggcggcgttc tcggcgtaga tgttggcgat gccgtgctcg tcgcggtaga
     2701 tgtggacggg cgcggtcagg ccgtggccgg tcagggcgac gcagagggcc gcctcttcgc
     2761 cctcgccggc cgtctcgcag gccgcgggca gttcgagcgc cttctcgccg tcggtatcgg
     2821 ggaaggggct gcgggcgacg gccatgccgc cggcaaacag gacaagggcc aggacggcca
     2881 gaacgccaac gatgattagc aggatgcgga cggcgcggtt cattcggttt ttcctctctc
     2941 ttcgacagc
//
LOCUS       DAOSMQ010000406         2937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_33748_length_2937_cov_3.629771, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000406 DAOSMQ010000000
VERSION     DAOSMQ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33748_length_2937_cov_3.629771"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK829_11955"
     CDS             complement(<1..231)
                     /locus_tag="PK829_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU41969.1"
                     /translation="MINVFLVEDNRFVADAVDGLLNTYDNIQLVGVATTGEEALTTLP
                     PTGVDIALIDLALPGMNGIDVIAALRQSQPALP"
     gene            complement(209..1768)
                     /locus_tag="PK829_11960"
     CDS             complement(209..1768)
                     /locus_tag="PK829_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU41970.1"
                     /translation="MSDPQQSPRAAGAAGEDRSQSEITTLLRLALKAAGLGTTITDNL
                     RGAFVTIDSTTAEIAGLPPGTTTLPPRRWLELIHPDDRAAVMALTEQLRPDGGAFTAT
                     FRLVRPDGATRWVRTAGAAIFSPDGRVPFAALMTDITDHTGDDTPPLVNLEARVRERT
                     AQLEAANTSLVAARDRFRTLFHSNPIPSVIYSPAEDRVVDANTAFLGFFGLPAERVVG
                     RAGDDLDILLDGSNRRGIVAAMPPGSGLQNVEIALPNRGGEPRIVLASLEPLELASGP
                     CILAGLVDITALKQAETQVRQMASQLSLAEQTERLRIAAILYDDLQQRLYALQVRLAA
                     TVAQAVRRDYNAAMAEAEQTRLLLDEALAMTRNLSVDISPPILQGESLYHALLWLSSR
                     LLEQYGVHVNVQLLSPWRLLPAGLRVALFQIVRELLFNVGKHAGVDTATVMLAQEDAR
                     VTLIITDTGAGFDPTAMKRPGGLYQMRRRIELLGGEMNLASRPGAGTTVTLIVPVNIE
                     ELGENDQRLSG"
     gene            2054..>2937
                     /locus_tag="PK829_11965"
     CDS             2054..>2937
                     /locus_tag="PK829_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41971.1"
                     /translation="MNRSPLSPALTARLAHDPQLAPRWAAVYAHLRALPRHLRQRLRR
                     ARLSLAAAALLLALGGAPRLAPLAAAPAVIITVANGEVDLDDNGLCSLVEAIDNANDT
                     TDGRGDEAGHDDCAAGNPAGADIVALPAGGAFTLTDYTSYDYGYTALPLITSTITISG
                     NGSTLQRTGGDELRFLAASGADLTLDHVTLQGGQAGDYYGGAVYAIDSTLTLTNCTLS
                     GNKAARGGALAVNGGAATITNSTLRDNDSDKGGAIYAYDAELALQTSTLSGNDAGAGQ
                     GGGVYSYAGRLALTGATVR"
BASE COUNT          437 a          969 c         1047 g          484 t
ORIGIN      
        1 gggcagcgcc ggctgcgact ggcgcagggc ggcgatcacg tcgatgccgt tcatgcccgg
       61 cagggccagg tcgatcaggg cgatgtcgac gcctgtgggc ggcagcgtag tcagggcttc
      121 ttcgccggtg gtggccacgc cgaccaactg gatgttgtcg taggtgttca gtagtccgtc
      181 gaccgcgtcg gcgacgaagc gattgtcctc aaccagaaag acgttgatca ttttcgccca
      241 attcctcgat gttgactggt acgatgagcg tgacagttgt gcccgcgccg gggcgcgagg
      301 cgaggttcat ttctccgccg agtagctcga tgcgccggcg catctggtag aggccgcccg
      361 gtcttttcat cgccgtcggg tcgaagccag cgcccgtgtc agtgatgatg agcgtcacgc
      421 gggcgtcctc ctgggccagc atgaccgtgg ccgtgtcgac gccggcgtgt ttgccgacgt
      481 tgaagagcaa ttcgcggacg atctggaaca gggccacgcg caaaccggcc ggcagtagcc
      541 gccaggggga tagtagctgc acgttgacgt gaaccccgta ctgctccagc aggcgcgagc
      601 tgagccagag cagagcatgg tagaggctct cgccttgcag gatgggcggg ctgatgtcca
      661 ctgacaggtt gcgggtcatg gccagcgcct cgtccagcag caggcgcgtc tgctcggcct
      721 cggccatggc ggcgttgtag tcgcggcgca ctgcctgggc caccgttgcc gccaggcgca
      781 cttgcagggc gtagaggcgc tgttgcaggt catcatacaa gatggcggcg atgcgcaacc
      841 gctcggtctg ctctgccagc gagagttgcg aagccatctg ccgtacctgg gtctcggcct
      901 gcttgagagc ggtaatgtcc accaggccgg ccagaataca cgggccactg gccagctcca
      961 ggggttccag cgacgccagg acgatgcgcg gctcgccgcc ccggttgggt agggcgattt
     1021 cgacgttctg caggccgctg ccggggggca tggcggcgac gatgccgcgg cggttggagc
     1081 cgtcgaggag gatgtcgagg tcgtcgcccg cccggccgac cacgcgctcg gctggcagac
     1141 caaagaagcc caggaaggcg gtgttggcgt ccacgacgcg gtcctcggct gggctgtaga
     1201 tgacgctggg aatggggttg ctatggaaga gcgtgcggaa gcgatcgcgg gcggcaacga
     1261 gcgacgtgtt ggctgcttcc agttgggcgg tacgttcgcg gacacgcgcc tccagattga
     1321 ccagtggcgg cgtgtcgtca ccggtgtggt cggtgatgtc cgtcatcagc gcggcgaagg
     1381 gcacgcggcc gtcggggctg aagatggccg cgccggcggt gcgcacccag cgcgtcgcgc
     1441 cgtcgggccg cacgaggcgg aacgtggccg tgaacgcgcc gccgtcgggc cgcagttgct
     1501 cggtcagggc catgacggcc gcgcggtcgt cggggtggat cagttccagc cagcggcggg
     1561 gcggcagcgt ggttgtgccg ggcggcaggc cggcgatttc ggccgtggtg ctgtcgatgg
     1621 tcacgaacgc gccgcggagg ttatccgtga tcgttgtgcc caggccggcc gctttgaggg
     1681 ccaagcggag gagcgtggtg atttcgctct ggctcctgtc ctcgccggcc gcgccggccg
     1741 cccgaggact ctgctgcgga tcgctcatcg agtgcgtcct gttcgccgcg gtgtctacca
     1801 ccattatagc ccactggctg cccaatgggc cattcttgct ttccggccca gggctttgcc
     1861 agagtcgtct ggcgactatt gacctgacag gtggccgtcg caggcgtggc cgcccacctg
     1921 acaggtcggc gcgcctttaa ccttgccgtg gcccgccgcc ccggccgatt gattgccctc
     1981 gcgccgctgg tgtatactgt tgccagagcc acggcgcggg cgcaagccgc gcccgcctgc
     2041 aaggagaatc gtcatgaacc gatcgcccct ctcacccgct ctgaccgccc gccttgccca
     2101 tgatccgcag ttagcgccac gctgggcggc cgtctatgcc cacctgcgtg ccctgccacg
     2161 ccacctgcgg cagcggctgc ggcgcgcgcg gctatcgctg gccgcggcgg cgctgctgct
     2221 ggccctcggc ggcgcgccgc ggctcgcccc ccttgccgcc gcgcccgcgg tcatcatcac
     2281 ggtggccaac ggcgaggtcg atctagatga taatggcctg tgcagcctcg tcgaggccat
     2341 cgacaacgcc aacgacacga ccgacggccg cggcgacgaa gcgggccatg atgactgcgc
     2401 cgccggcaac cccgccgggg ccgacatcgt tgccctgccg gccggcggcg cgttcaccct
     2461 gaccgactat accagctatg actacggcta caccgccctg ccgctgatca cctcgaccat
     2521 caccatcagc ggcaacggct cgacgctgca acggacgggc ggcgacgagc ttcgctttct
     2581 ggccgcctcc ggggctgacc tgacgctcga ccacgtgacg ctgcaaggcg gccaggccgg
     2641 cgactattat ggcggcgcgg tctatgccat cgacagcacg ctgacgctga ccaactgcac
     2701 gcttagcggc aacaaagccg cgcggggcgg cgcgctggcc gtcaatggcg gcgcggcgac
     2761 gattacgaat tcgaccctgc gcgacaacga cagcgacaag ggcggggcca tctacgccta
     2821 cgatgccgag ctggcgttgc agaccagcac cctcagcggc aacgacgccg gggcggggca
     2881 ggggggcggg gtttatagtt acgccggccg cctggcgcta acgggcgcga ctgtgcg
//
LOCUS       DAOSMQ010000407         2922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_33984_length_2922_cov_4.307290, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000407 DAOSMQ010000000
VERSION     DAOSMQ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33984_length_2922_cov_4.307290"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..261
                     /locus_tag="PK829_11970"
     CDS             22..261
                     /locus_tag="PK829_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU41972.1"
                     /translation="MNDTEERVRAIIVDLLGVDEDQVVIDARFREDLEADSLDLVELI
                     MEFEKEFAGEISDEEAQKITTVGEAVYYINSRMRS"
     gene            complement(348..989)
                     /locus_tag="PK829_11975"
     CDS             complement(348..989)
                     /locus_tag="PK829_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41973.1"
                     /translation="MDTTPAGHFPRLTRRDGLALGLTAIFIAYFLVWLPGPGAGLSLI
                     GVELGEWIKFLGVGGGRNWFYVPPITLGLLLTLWTATWPNRRARTWLARGLAVAVALL
                     AFPAVAAITSEPPSEWLLRLVLIGAVAAVAVLAALLAARPKLAAWVWLGMGAVAVAGA
                     VGPTAQYLAVRPVIAGALMQPLGIGAGVWFNALGHALVAVVGLAAFRAARQKK"
     gene            complement(1002..1487)
                     /locus_tag="PK829_11980"
     CDS             complement(1002..1487)
                     /locus_tag="PK829_11980"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOU41974.1"
                     /translation="MNARRVNESRVTFTQPMGIDAANALGNVHGGIIMKICDEAGAYA
                     AMKHARRPVVTVSVDSMSFHSAVNIGNLLTVNAEVTWVGRSSMETRILVTAEELMSGR
                     VTHTNTAYFVYVALDERGRPAEVPPLLLETDEERRHFEEAAQRQARRLERRGDERAAG
                     R"
     gene            complement(1465..2232)
                     /gene="trmD"
                     /locus_tag="PK829_11985"
     CDS             complement(1465..2232)
                     /gene="trmD"
                     /locus_tag="PK829_11985"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOU41975.1"
                     /translation="MRIDVLTLFPAMFSAYLDESILRRAQTAGLIAIGVHNIRDHAAG
                     RHRVTDEPPFGGGGGMVLKPEPLFAAAEAIIGGTSPGPRRILLSPQGRSLTQALATEL
                     AGEAWLLLLCGRYEGVDERVRQHLVDDEVSIGDYVLTGGELAALVLIDAVARHVPGVL
                     GHEHAAVRDSFAMGLLEGPHYTHPVSFRGWDVPAVLRSGHAGRIDRWRREAALRRTWQ
                     RRPDLLLAADLDEADRRFLMILARETVEPNDERTTSE"
     gene            2353..>2922
                     /locus_tag="PK829_11990"
     CDS             2353..>2922
                     /locus_tag="PK829_11990"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU41976.1"
                     /translation="MRILITGANGQLGVALQQTLADHELTLIDLPAVDITNRAALAAA
                     FAAARPELVVHCAAYTDVDGCARNPELAYQVNAYGTQNVALACQSAGTALVHISTNEV
                     FAGDEPGGYEEWMPLAPRNPYGRSKAAAEAHVRALLPRAYIVRTAWLYAPGGRNFIHA
                     ILRRARETGTVRVVTDEVGNPTYAPDLAAA"
BASE COUNT          552 a         1018 c          932 g          420 t
ORIGIN      
        1 acattgaagg agaggcagtc aatgaatgac acggaagaga gagtgcgggc gatcatcgtc
       61 gacctgctgg gcgtagatga agaccaggtg gttatagacg cgcgctttcg cgaagatctg
      121 gaggcggatt cgctcgactt ggtcgagcta atcatggagt tcgagaagga attcgccggt
      181 gagatctcgg atgaagaagc ccagaagatc accaccgttg gggaagccgt ttactacatc
      241 aatagccgga tgcgctccta atagcatcac ccgtcaacac cacagctgcg gctgcttttc
      301 gcttcacaaa tgctgacagc cttccctgat ggacagatgg aaggctgtca ctttttttgc
      361 cgcgcggccc ggaaggcggc caaacccacg acggccacca gcgcgtgccc cagtgcattg
      421 aaccacaccc ccgcgccaat gcccaacggc tgcatcaacg cgccggcgat gaccggccgc
      481 actgccagat actgcgccgt tggcccaacc gcgccggcga cagccaccgc gcccatcccc
      541 agccacaccc acgccgccag tttgggccgc gcggccagga gcgccgccag caccgcaacc
      601 gcggccaccg ccccaatcag caccagccgc agcaaccact cactgggcgg ctctgaagtg
      661 atggcggcga cggccgggaa ggccagcagg gccacagcca cggccagccc gcgcgccagc
      721 caggtgcgcg cccgcctgtt gggccaggtc gcggtccata gggtcagcag caaacccaaa
      781 gtgatggggg gcacatagaa ccagttccgg cctccgccga cgcccaggaa cttgatccac
      841 tcgcccaact cgacgccgat gaggctcagg cccgcgcccg gccccggcag ccagaccaga
      901 aaataggcga tgaagatggc cgtcagcccc agagccagcc cgtcgcggcg ggtcagccga
      961 ggaaagtggc cggcaggggt ggtgtccatg ggccgcgttg cctagcgacc ggcggcgcgc
     1021 tcgtcgccgc ggcgctccag acgccgcgct tgtcgttgcg ccgcttcctc gaagtgccgg
     1081 cgctcctcgt cggtctccag gagcaggggc ggcacttcgg ccggacggcc gcgctcatcc
     1141 agagcgacgt agacgaagta ggcggtgttg gtgtgggtca cccggccgga catcaactcc
     1201 tcggccgtca ccagaatgcg cgtttccatc gacgagcgcc ccacccaggt gacttcagcg
     1261 ttgaccgtca gcaggttgcc aatgttgacc gccgaatgaa agctcatcga atcgaccgag
     1321 acagtgacga ccggccggcg ggcgtgcttc atggccgcat acgcccccgc ttcgtcacaa
     1381 atcttcatga taatgccgcc gtggacgttg ccgagggcgt tggctgcgtc gatgcccatc
     1441 ggctgggtaa aggtcacccg tgattcattc actcgtcgtg cgttcatcgt tcggttccac
     1501 tgtctcccgg gccaagatca tcagaaagcg ccggtcggcc tcgtcgaggt cggcggccag
     1561 cagcaggtca ggccggcgct gccacgtccg ccgcagcgcg gcctcacgcc gccaacggtc
     1621 gatgcgcccg gcatggccgg aacgcaacac ggccggcacg tcccaaccgc gaaatgacac
     1681 cgggtgcgtg taatgcggcc cttccagcag gcccatagca aagctgtcac gcacggcggc
     1741 gtgttcatgg cccaggacgc caggcacgtg gcgggctacg gcgtcgatga gcaccagggc
     1801 ggccagttcg ccgccagtca gaacgtagtc gccgatggag acctcgtcat cgaccagatg
     1861 ttggcgcaca cgctcatcaa cgccctcata ccggccgcac agcagcaaca gccacgcttc
     1921 cccggccaac tcggtggcca gcgcctgggt cagcgatcgc ccctgaggcg acagcaggat
     1981 gcgccgcggc cccggcgagg tcccgccaat gatggcctcg gccgcggcga agagcggctc
     2041 cggcttcagc accatgccgc cgccgccgcc aaaaggtggc tcatcggtca cgcggtggcg
     2101 gccggcagcg tggtcgcgga tgttgtggac gccgatggca atcagccccg cggtctgggc
     2161 gcgtcgcagg atactctcgt ccagataggc cgagaacatg gcggggaaga gggtcaggac
     2221 gtcgatgcgc atcatgcgtt ggcggcgcgg aagagcgccc gacaggagta tcaaggggaa
     2281 gcgaagcgct gtcaagcgaa aggctggcga ccaattgaca agaaccggcg cgcggcacac
     2341 aattagcgtc acatgcgcat cctgattacc ggagccaacg gccaactcgg cgtcgcccta
     2401 cagcaaacgc tggccgacca cgaactgacc ctcatcgacc tgcccgccgt agacattacc
     2461 aaccgcgcgg cgctggcggc tgccttcgcc gcggccaggc cggagcttgt cgtccactgt
     2521 gcggcctaca ccgacgtaga cggctgcgcg cgcaacccgg agttggccta tcaggtcaat
     2581 gcttacggca cgcagaacgt cgccctggcc tgccagagcg ccggcacggc cctggttcac
     2641 atcagcacca acgaggtctt cgccggtgac gagccaggcg gctatgagga gtggatgccc
     2701 ctggcgccgc gcaaccccta tggccgctcc aaggccgccg ccgaggcgca cgtccgcgcc
     2761 ctgttgcccc gcgcctacat cgtccgcacg gcctggctct atgcgcccgg cgggcgtaac
     2821 ttcatccacg ccatcctgcg gcgcgcccgc gagacgggca ccgtgcgtgt cgtcaccgac
     2881 gaagtgggca acccgacgta tgccccagac ctggccgcgg cc
//
LOCUS       DAOSMQ010000408         2914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_34107_length_2914_cov_4.727877, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000408 DAOSMQ010000000
VERSION     DAOSMQ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34107_length_2914_cov_4.727877"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PK829_11995"
     CDS             complement(<1..461)
                     /locus_tag="PK829_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU41977.1"
                     /translation="MHLYLIRHAQSANNHAYATGGERQPDPPLTELGHRQARALADYL
                     RQQPDSARTAYAERHGRGGIVLTHLYTSLMLRAVTTAAYIAEATGLPLHAWTEIHEIG
                     GLHGIAPETGEVVGVPGPGRAYFAAEHPTLILPDTLTDAGWWNRDKEPREE"
     gene            complement(466..2487)
                     /locus_tag="PK829_12000"
     CDS             complement(466..2487)
                     /locus_tag="PK829_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU41978.1"
                     /translation="MNINRPIVLRALLLALVLPLLLALPGRATAQDAAGAFVPAPCMF
                     TLDLGVTKPSGEKLGFTCGYVVVPVRHAAPDGPTLRLPVAVRRATGSPSDPLILAQGG
                     PGGDAFEVFSILVPSSSVAASRDIIIFNQRGTPYAEPELTCPETDAVLADILAADTDA
                     GEQLYEDALAACHARLLAEGIDLSAFNSLENAADVPMIAHALGYEEYNFYGVSYGTLL
                     GLHLMRTHPEGLRSVILDSVVSTDINFIAESAASEDRVFGQVFAACAANPTCAAEYPN
                     LEERFYAVLAQFDAHPVTLTLTDPDTGRDYPTYLDGRGLRSVLWQLLYVARMAAVIPK
                     LVTDLEAGDLRYLQAMWPLLVFDQLVAEGMYYSVVCAEDADVDPAAVPLNNLRPEIAA
                     TARDELQSFVETCALWHVEQLPPSVDAPVVTDIPTLLLSGRFDPVTPPIFAQAAAAGL
                     SHATVIFHPTAAHGVAFQDACVNDIMVRFLDNPDLVPDSSCLQTVSTLNFVPPNAPTF
                     PPLAGVNSLKPRTLIVFGLAAVLVLLLASAFVVWPLVYIVRAFGEKRAERAPAARRLR
                     WLSRGLMLAFAAVAVVLGLGLLVAIVASLADLTLATGLALPPAAAPLLWLPLALLLLA
                     VGIVVVAALLWRQRDSGSTAGKAYYTVLALCAVALVILLASQGLLLPPL"
     gene            2666..>2914
                     /locus_tag="PK829_12005"
     CDS             2666..>2914
                     /locus_tag="PK829_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41979.1"
                     /translation="MIAIRPLSHFDPAGFVALASGYTAVAIYRVARTESDAETTLRLT
                     LEPLPAPREFGFPYHADELRHYAECVPGPFCLGAYDGER"
BASE COUNT          476 a          884 c         1133 g          421 t
ORIGIN      
        1 cactcctcgc gcggttcctt gtcccggttc caccagcccg cgtcggtcag cgtgtcgggc
       61 aagatcagag tgggatgctc ggcggcgaag taggcccggc ccggcccggg cacgccgacg
      121 acctcgcccg tctccggggc aatgccgtgc aggccgccga tctcgtgaat ctccgtccag
      181 gcgtggaggg gcaggccggt ggcctcggca atgtaggcgg cggtggtgac ggcgcgcagc
      241 atcaggctgg tgtagaggtg ggtgaggacg atgccgccac ggccgtgccg ctcggcatag
      301 gcagtgcgag cgctgtccgg ctgctggcgc aggtagtcgg ccagggcgcg ggcctggcga
      361 tggcccagct cggtcagcgg cgggtcgggc tggcgctcgc cgccggtggc gtaggcgtgg
      421 ttgttggccg attgggcgtg gcgaatgagg tacaggtgca tagcgctata gcggtggtag
      481 taatagcccc tggctggcga gcaggatgac cagagcgacg gcgcagaggg ccaggacggt
      541 gtagtaggct ttgccggcgg tggagccgct gtcgcgttgc cgccagagga gggcggcgac
      601 gacgacgatg cccacggcca atagcaacag cgccagcggc agccacagca gcggcgcggc
      661 ggccggcggc aaggccaggc ccgtggccag tgtcaggtcg gccagcgagg cgacgatggc
      721 caccagcagc cccagcccca ggacgacggc cacggcggca aaggcgagca ttagcccgcg
      781 actgagccag cgcaggcggc gggccgccgg cgcgcgctcg gcgcgcttct cgccaaaggc
      841 gcggacgatg tagacgagcg gccagacgac gaaggccgac gccagcagca gcacgaggac
      901 ggccgccagc ccaaagacga tcagcgtgcg cggcttaagg ctgttcactc cggccagcgg
      961 cgggaaggtg ggcgcgttcg gggggacgaa gttcagcgtg ctaacggttt gcaggcaact
     1021 gctgtcggga accaggtcgg ggttgtccag gaagcgaacc atgatgtcgt tgacgcaggc
     1081 atcctggaag gcgacgccgt gcgcggccgt tgggtgaaag atcacggtgg cgtggctcag
     1141 gccggcggcg gcggcctggg cgaagatggg cggtgtcacc gggtcgaagc ggccggagag
     1201 gagcagcgtg ggaatgtcgg tgacgacggg cgcatcgacc gacggcggca gttgttcgac
     1261 gtgccacagg gcgcaggttt cgacaaagct ttgcagctcg tccctggccg tggcggcgat
     1321 ctccggccgc aggttgttca acggcacggc ggcggggtca acgtcggcgt cttcggcaca
     1381 gacgaccgag tagtacatcc cctcggctac gagctggtcg aagaccaaca gcggccacat
     1441 ggcttgcaga tagcgcaggt cgcccgcttc caggtcagtg acgagcttgg gtatgacggc
     1501 ggccatcctg gccacgtaga gcagttgcca gagcacgctg cgcaggccac ggccgtcgag
     1561 gtaagtcggg tagtcgcggc cggtgtccgg gtcggtcaac gtcagggtga cggggtgggc
     1621 gtcgaactgg gccaggacgg cgtagaagcg ctcttcgagg ttggggtatt cggccgcgca
     1681 ggtcgggttg gcggcgcagg cggcgaagac ctggccaaag acgcgatcct cactggccgc
     1741 ggactcggca atgaagttga tgtcggtgga aacaacgctg tcgaggatga cgctgcgcag
     1801 cccttcgggg tgggtgcgca tcaggtgcag gcccagcagg gtgccatagg acacgccgta
     1861 gaagttgtac tcctcatagc ccagcgcgtg ggcgatcatg ggcacgtcgg cggcgttctc
     1921 caggctgttg aaggctgaca ggtcgatgcc ctcggccagc aggcgggcgt ggcaggcggc
     1981 cagggcgtcc tcgtagagct gctcgcccgc gtcggtgtcg gcggcaagga tgtcggccag
     2041 gacggcgtcg gtctccggac aggtgagttc cggctcggcg tagggcgtgc cgcgctggtt
     2101 gaagatgatg atgtcgcggc tggcggccac tgaggaggac ggcacgagga tcgagaagac
     2161 ctcgaaagcg tcgccgccgg gaccgccctg ggccaggatg agcgggtcgg acgggctgcc
     2221 cgtggcgcgg cggacagcca cgggcaggcg cagcgtcggg ccgtcgggcg cggcgtggcg
     2281 cacgggcacg acgacgtagc cgcacgtgaa gcccagcttc tcgccgctgg gcttggtcac
     2341 gcccaggtct agcgtgaaca tgcacggcgc gggcacgaac gcgccggcgg cgtcctgggc
     2401 ggtggcgcgg ccgggcaagg ccagcagcag cggcaagaca agggcaagga gcaaggcgcg
     2461 gaggacgatg gggcgattga tgttcatagt ggttccacca gcaatacggc ggccgtggcc
     2521 cccggttgcg atggttgtag cagagggggg cgttctgggc aagcgagggg caggaagcga
     2581 cagggcctgg ccctcaactt ggccgcctgc caggatgctg ccataatccc tatgcggccg
     2641 agatcagccg cgccggaaga ccgtcatgat tgccattcgt cccctgtccc acttcgaccc
     2701 cgctggcttc gttgctctgg ccagcggcta tactgccgtg gccatctacc gcgtcgcccg
     2761 caccgagagc gatgccgaga cgaccctgcg gctgacgctg gaaccgctgc ccgccccgcg
     2821 cgaattcggc tttccctatc acgccgatga actgcggcac tatgccgagt gtgtccccgg
     2881 cccgttttgc ctgggggcct acgacggcga gcgg
//
LOCUS       DAOSMQ010000409         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_34659_length_2879_cov_5.190510, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000409 DAOSMQ010000000
VERSION     DAOSMQ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34659_length_2879_cov_5.190510"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            440..1510
                     /locus_tag="PK829_12010"
     CDS             440..1510
                     /locus_tag="PK829_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOU41980.1"
                     /translation="MLNPNLLWWIAGFLIVLTPIVIIHEGGHFFAAKLSKIRVEEFGF
                     GLPPRAVKLAERNGTIYSLNWIPVGGFVRPAGEDDPDVPGGLASASKRARLFVLAAGA
                     GANFLTAILFMWLAVLFSPSVMIDAVNPASPAASSDLRVGDVVLAVDGQEVSDAGMLI
                     STVSERAGQQIDLLVRRGQEEVTINVRPRLPGEYDAATEGALGIGLGDGPALRQVGPV
                     EAMGQSLRTIGEYLTLYARLPSMLINREITAEEARPVSIIGISQIAGGALESSVVSRN
                     PYRLLIMAAFISVALGLTNLLPIPALDGGRILFVLIEAIRGRRIEPQREGMVHLIGML
                     MLLGLMAFLIFQDIFNPIVPLQ"
     gene            1532..2533
                     /locus_tag="PK829_12015"
     CDS             1532..2533
                     /locus_tag="PK829_12015"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOU41981.1"
                     /translation="MTNQRQQSFTSVLSALFNGDDVPVHLIYRLSDMEMAEFNQFQRA
                     WASVDEARRAAIVRHMAEIAEENTLIDFTPVFAYLFDDKYASVRQGALDGVWDTTDPA
                     LIRPIINLLQTDPSVVVRAAAARALAHYVLLAEWGQIAPGVVTPIVDALLAEYEQPKA
                     NEEVKRAALEALASANHPRVPELIRNAYEDGSNEMQLSALFAMGLNADDRWLSIVSDE
                     MESPSADFRAEAARAAGAIGNPEAIDGLEQLLHDEDVEVAIAAVYALGQIGSERATEL
                     LNRLADDPAYEDLYDAIDEALEEMDWMGGEFDLLALPDDEDEDENDDFAIDIDPRAN"
     gene            2530..>2879
                     /locus_tag="PK829_12020"
     CDS             2530..>2879
                     /locus_tag="PK829_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU41982.1"
                     /translation="MSVFVAVLTQNILPIMLVASFGYWLRRRFEVHLSTLTSVVFNVL
                     SPCLVFSSLVTSQLPGDELLHLAAFAAGTILMMGGLALLLSRLLRLGRAATAAVVLIA
                     MFTNGGNYGLTLLQ"
BASE COUNT          462 a          932 c          930 g          555 t
ORIGIN      
        1 cgccgttttt cgccagactc acttgaaggg gccaaagctc agagggtggt cacccctggg
       61 aggcatccgc tgaactgtcg attttcgccg gctgtggccg gcttggcccc gcgtcgccgc
      121 ggggaacctc cacctcgtca cccaccggcg ttgtcgccgc ggtgggtcat gcgctgtctc
      181 gcccctcgga cggccgacca gagcagggcc accgtccgct attcggttgt tgtggtagag
      241 catagcggcg gggcaggggg ttgtcaaggc ggcgggcgtg aaagtggtga gagtgatgtt
      301 agatctgcgg cgcggtcttg gatggcgggc caccagggaa tataattctt tcttgacgcg
      361 atcgtcttgg ctggcctgtc agcggtacct ggccatatag cgccttcacc tgggccgcgc
      421 gaacgcgggg acaaagcgaa tgttgaatcc aaacttactg tggtggatcg ccgggtttct
      481 gattgtcctg acgcccattg tcatcatcca cgagggtggc cacttcttcg ccgccaaact
      541 gagcaaaatc cgcgtcgagg aatttggctt tggcttgccg ccgcgggcgg ttaagctggc
      601 cgagcgcaac ggcacgatct actcgctcaa ctggattccg gtcggcggct tcgtgcggcc
      661 ggcgggcgag gacgaccccg acgtgcccgg cggcctggcg tcggcgtcga agcgggcgcg
      721 gctgttcgtg ctggccgctg gggccggagc caacttcctg acagcgattc tgttcatgtg
      781 gctggctgtg cttttctcgc cgtcggtcat gatcgacgct gtgaacccgg cctcgccggc
      841 ggccagcagc gatctgcgcg tgggcgatgt tgtcctggca gttgacggac aggaagtcag
      901 cgacgcgggc atgttgatca gcacggtctc agagcgggcc gggcagcaga tcgacctgct
      961 ggtgcggcgc gggcaggaag aagtgacgat caacgtccgg ccgcgcctgc cgggtgaata
     1021 tgatgccgcg acggagggcg cgctgggcat cggcctgggt gacggcccgg cgctgcgtca
     1081 ggtcggcccg gtcgaggcga tgggccagtc cctgcgcacg atcggcgaat acctgactct
     1141 ctacgcccgc ctgccatcca tgctcatcaa ccgcgagatc accgccgagg aagcgcgccc
     1201 agtcagcatc atcggcatta gccagattgc cggcggcgcg ctggagtcgt cggtcgtctc
     1261 tcgcaatccc taccgtctgc tcatcatggc cgcgttcatc agcgtcgccc tcggcctgac
     1321 gaacctgctg cctatccctg ccctggacgg cggccgtatt ctctttgtgc tcattgaggc
     1381 cattcgcggc cggcgtattg agccgcagcg cgagggcatg gtccatctca tcggtatgct
     1441 catgctcctg gggctgatgg ccttcctcat cttccaggac atctttaatc ccattgtgcc
     1501 gctgcaatag cgctcggctg gtaacccatt catgaccaac caacgccaac aatctttcac
     1561 gtctgtcctg tcggccctgt tcaacggcga cgacgtgccc gtccacctga tttaccgcct
     1621 gtccgacatg gagatggcag agttcaacca gttccagcgc gcctgggcca gcgtggacga
     1681 ggcgcgccgg gcggccattg tccggcatat ggccgagatt gctgaggaga acaccctcat
     1741 cgatttcacg cccgtctttg cctatctgtt cgacgacaag tacgcgagcg tgcgccaggg
     1801 cgcgctcgat ggcgtctggg ataccactga tccggccctg atccggccga tcatcaacct
     1861 gctccagacc gaccccagtg ttgtcgtgcg cgccgcggcg gcgcgggcgc tggcccacta
     1921 cgtgctgctg gccgagtggg gccagatcgc gcccggcgtg gtcacgccga ttgtcgatgc
     1981 gctgctggcg gagtatgagc agccgaaggc caacgaggaa gtcaagcggg ccgctctgga
     2041 agccctggcc tcggccaatc atccccgcgt gccggagctg atccgcaacg cctacgagga
     2101 cggctccaac gagatgcagc tcagcgccct ctttgccatg ggcctgaacg ccgacgaccg
     2161 ctggctgtcg atcgtcagcg acgagatgga aagtccctcg gccgactttc gcgccgaagc
     2221 ggcccgcgcc gccggcgcca tcggcaaccc agaggcaatc gacggcctgg agcagttgct
     2281 gcacgatgag gacgtcgaag tcgccatcgc cgccgtctac gccctgggcc agatcggcag
     2341 cgaacgcgcc accgagctgc tgaaccggct ggccgatgat cccgcctacg aagacctcta
     2401 cgacgccatc gacgaagccc tcgaggagat ggactggatg ggcggcgagt tcgatctgct
     2461 ggccctgccc gatgatgagg acgaagatga gaacgacgat ttcgccatcg atatcgaccc
     2521 gcgcgccaat tgagcgtctt tgtcgccgtc ctgacacaga acatcctgcc gatcatgctg
     2581 gtggcctctt ttggatactg gctgcggcgg cggtttgaag tccatctcag caccctcacc
     2641 agcgttgtct tcaacgtcct aagcccttgc ctggtcttct cctcgctcgt caccagccag
     2701 ttgcccggcg acgagttgct gcacctggcc gccttcgcgg cgggcacgat cctgatgatg
     2761 ggcgggctgg ccttgctgct gtcgcggctg ctgcgcctgg ggagagcggc gacggcggcc
     2821 gtggtgctca tcgccatgtt caccaacggc ggcaactatg gcctgactct gctccagtt
//
LOCUS       DAOSMQ010000410         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_34687_length_2878_cov_3.815090, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000410 DAOSMQ010000000
VERSION     DAOSMQ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34687_length_2878_cov_3.815090"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PK829_12025"
     CDS             complement(<1..319)
                     /locus_tag="PK829_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOU41983.1"
                     /translation="MNAYQTLLDRVHELNDLGKANALLSWDREVNMPRAGTPERIAQM
                     TTLSSLIHRLSTSDEMGELIERAAAEIDGADYDSDEAALIRLLRRNYADERKLPADYV
                     ARAA"
     gene            complement(356..925)
                     /locus_tag="PK829_12030"
     CDS             complement(356..925)
                     /locus_tag="PK829_12030"
                     /inference="COORDINATES: protein motif:HMM:NF025802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HOU41984.1"
                     /translation="MIRPHHLGDQSTFLGQNCALCKQEFEAGDDIVICPEDGSRHHVH
                     CWQANHNHCTAYGCRGQGSVGAPAPDLTFQPATEQTPVADAQPEEEPARPPSRPRIVP
                     IMPPPPRARPTAARPIPNAPGSKVRTLPAGSFGCVRGCFIAVLLLVVVLVALACAGAW
                     FFTVRTTPAPASAILPLLTGLAALPFRLS"
     gene            complement(971..1396)
                     /locus_tag="PK829_12035"
     CDS             complement(971..1396)
                     /locus_tag="PK829_12035"
                     /inference="COORDINATES: protein motif:HMM:NF025802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HOU41985.1"
                     /translation="MDEQEHPPIRITEHDISEANQLSLSCPICGNAVENNTDGEALVP
                     VVCDKCGTLYHRACWEGSGGKCAVLGCGSVKFHIYGKDTRPVLKVKYTDLPASSANGR
                     AVPSPQTRRLKEEQRRQVQRMTLFQRFWKWLLDQIRINP"
     gene            complement(1529..2608)
                     /locus_tag="PK829_12040"
     CDS             complement(1529..2608)
                     /locus_tag="PK829_12040"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HOU41986.1"
                     /translation="MTNETTPTYAAQLTLSLPGQGPSATVTVTLAPADALPVEGRSKP
                     LSECTLAELRAYASELEADAWDAYDAITLHQLQEEAKIDIVLRPLDASGQAGAEVEDW
                     LQQAIILPPAPVEETEPAEEPPTAGAPAAEPGAAATAQAGVSAPELEPAPAPTPPQPQ
                     PIVAQAAADSAAVVEPPTIIASQARVRVAGGRLPLAYAAGAAVDILLDEMPLRAMQAH
                     ALSSLDREVAGVMIGRQPEKQPDGRYIVHVIDSIIAKHTVMHGASVTYTPESWRHLND
                     TLWERYPDESAVMVGWYHTHPGFGIFLSGMDLFIHQNFFTQIWHVAYVLDPRARNSGF
                     FCWNRQKTKVNRYDFQWPDWAAGSW"
     gene            complement(2647..>2878)
                     /locus_tag="PK829_12045"
     CDS             complement(2647..>2878)
                     /locus_tag="PK829_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41987.1"
                     /translation="PGARRYQIDVRPAGTETWTMLDRRQGNTRLGQLAQWDTAELPTG
                     QYELRLTPVDLNNIRLTDSSSCAITLGLINEN"
BASE COUNT          424 a          868 c         1046 g          540 t
ORIGIN      
        1 cggccgcgcg ggccacgtag tcggccggca gcttgcgctc gtcggcgtag ttgcggcgca
       61 acaggcggat gagggcggcc tcgtcgctgt catagtccgc gccgtcgatc tccgccgcgg
      121 cccgttcgat cagttcgccc atctcgtccg acgtgctcag gcggtggatc aggctgctaa
      181 gggtggtcat ctgggcgatg cgctcgggcg tgccggcacg gggcatgttc acttcgcggt
      241 cccagctcag cagagcattg gctttgccga gatcgttcag ttcgtgaaca cggtcaagga
      301 gggtttgata ggcattcacc ggaaatcgtc tcctttgtaa gattgtggag tggggtcagg
      361 aaaggcgaaa aggcagcgct gccagcccgg tcagcagcgg caggattgcg ctggccggcg
      421 cgggcgtcgt gcgcaccgtg aagaaccatg cccccgcgca ggccagggcc acgagaacga
      481 cgaccagcag caggacggcg atgaaacagc cgcggacaca gccgaagctg cccgccggca
      541 gcgtgcggac tttggagccg ggcgcgttgg gaatggggcg ggcggccgtc gggcgggcac
      601 gtgggggcgg cggcatgatg ggcacaatgc gcggccgcga cggtgggcgg gccggctctt
      661 cctccggctg cgcgtcggcc actggcgtct gctccgtcgc cggctggaag gtcagatcgg
      721 gcgcgggcgc gccgacgctc ccctggccac ggcaaccgta agcggtgcag tggttgtggt
      781 tggcctgcca gcaatggacg tggtggcggc tgccatcctc cgggcaaata acgatgtcgt
      841 cgccggcctc gaactcctgt ttacacaggg cgcagttctg ccccagaaag gtggattggt
      901 cgccgaggtg gtgcggacgg atcatgatga gcgacccgac ggtttcgccc gaatccgtcg
      961 ggtctggtgt ctaggggtta atgcgaatct ggtcgagcag ccacttccag aagcgctgga
     1021 agagggtcat gcgctgcacc tggcggcgct gctcttcctt cagacggcgc gtctgggggc
     1081 tggggacggc gcggccgttg gccgacgacg ccggcaggtc ggtgtacttg accttcagca
     1141 ccggccgggt gtccttgccg tagatatgaa acttgacact gccgcagccc agcacggcgc
     1201 atttaccgcc gctgccttcc cagcaggccc gatggtaaag cgtgccgcac ttgtcgcaga
     1261 caacgggcac cagcgcctcg ccgtcggtgt tgttctccac cgcgttgccg caaatgggac
     1321 aggagaggct cagttggttg gcctcagaga tgtcgtgctc cgtaatgcgg atgggcggat
     1381 gctcttgctc gtccataact cgaaacctgg aagcggcgag ctggtcgtgt gtctcggtcg
     1441 tccgggctgc ctgtgggcca ggggtcgctc gcgttcctga ggtctgatta tacatcattt
     1501 tggcgctcca atacggaacc ggttcgcgct accacgaccc ggcggcccag tcgggccact
     1561 ggaagtcata gcggttgacc ttcgtcttct gccgattcca gcagaagaag ccgctgttgc
     1621 gggcccgcgg gtcgagaaca taggccacgt gccagatctg ggtgaagaag ttctggtgga
     1681 tgaacagatc catgcccgac agaaagatgc cgaagccggg gtgggtgtgg taccagccga
     1741 ccatgacggc gctctcgtcg gggtagcgct cccacagggt atcgttgaga tgtcgccagc
     1801 tttcgggcgt ataggtgacg ctggccccgt gcatgacggt gtgcttggca atgatgctgt
     1861 caatgacgtg gacgatgtag cggccgtcag gctgcttttc aggctggcga ccgatcatca
     1921 cgccagcgac ctcgcggtcg aggctgctca gggcgtgggc ctgcatggcc cgtagcggca
     1981 tctcgtccag caggatgtct acggccgcgc cggcggcata ggccagcggc agccgcccgc
     2041 cggccacgcg cacgcgcgcc tgactggcaa tgatggtcgg cggctcgacc acagcggcgc
     2101 tgtccgcggc cgcctgggcc acaattggct gtggctgtgg cggcgtgggg gcgggcgcag
     2161 gctccagttc gggcgcggat actccggcct gggctgttgc ggctgcccca ggctcggccg
     2221 cgggcgcgcc ggctgtgggt ggttcctcgg ccggttcggt ttcttcgacc ggcgcgggcg
     2281 gcaggatgat ggcctgctgt agccagtctt ctacctctgc gccggcctgg ccactggcgt
     2341 ccagcggccg cagcacgata tcgattttgg cctcctcctg aagttggtgg agggtaatgg
     2401 cgtcgtaggc atcccaggca tcggcttcca gttcgctggc gtaggcgcgc aattcggcca
     2461 gggtgcactc agacaggggc ttgctgcggc cctcgaccgg cagggcgtcg gccggcgcga
     2521 gcgtgactgt gaccgtcgcc gatggcccct ggccggggag gcttagcgtt agctgggcag
     2581 cataggtagg ggtggtttcg ttggtcattt cggatttcgt cgccacggcc agcgcggccg
     2641 tggcggtcag ttctcgttga tcagcccgag cgtaatggcg caggagctgc tatcggtcag
     2701 gcgaatgttg ttcagatcga ccggcgtgag acgcagttcg tactgcccgg ttggcagttc
     2761 ggccgtgtcc cattgcgcca gttggcccag gcgcgtgttg ccctgacggc ggtcgagcat
     2821 tgtccacgtt tctgtgcccg ccggccgcac gtcgatttgg tagcggcgcg cgccgggg
//
LOCUS       DAOSMQ010000411         2872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_34784_length_2872_cov_4.243166, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000411 DAOSMQ010000000
VERSION     DAOSMQ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34784_length_2872_cov_4.243166"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1372
                     /locus_tag="PK829_12050"
     CDS             38..1372
                     /locus_tag="PK829_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOU41988.1"
                     /translation="MRKYRLPLLLGLLLAGIITVRFLPAMLRTMPTRYAMRLPAPLQA
                     LALPAASTPILPTAAAPRDAAALLGNAAVPATATPPPSPTPQTVAAGFATAAPATSPA
                     TPTLAPTPTATPWPVPSAARMTGFTHVFQEWNNCGPATLVMTLTHFGRVLSQRDTALV
                     LRPDPEDRNVTPHEMADFVNNTQDGLHAVARINGDLRLLKQLLANNVPVIIEIGFDPP
                     GEYQWLGWYGHYLLPVAYDEAAGQFWVYDSWLGTSEVPQTNADANGRVLTEAELDAFW
                     TEFNHSYIVVYETAQETLVQQLLGDTLNDDAMWAASLARAQTDAARAPDNAFYWFNLG
                     STYNALGRYDEAAVAFDEARAIGLPWRMLWYQFGPYEAYYRVGRYDDVILLTQTTLQD
                     RPYFEESYYYLGLAQAALGDSAAARENLQKAHTLNPSFAPAEKALAELPADG"
     gene            1411..2841
                     /locus_tag="PK829_12055"
     CDS             1411..2841
                     /locus_tag="PK829_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOU41989.1"
                     /translation="MRNRTKSIVLLIAVAQLGLIIGLLALPTVVQAIPGRYRVWLQEN
                     HPALGRVSEGVIHQVAPVATALPAPQQVAESAIDIGSLIAAQPSTTLASAPDETELIG
                     VPNGAASPAAPAVTVAVEPTAAPTEPALTPTPAATATPAPTPTPMPLPDRAVLEGMSI
                     VKQSFNNCGPANLTQVLNWYDSPITQDDVASYLKPNPEDRNVSPWQIADYVNEQMPGF
                     KAIARSGGDLDMLRRFLASGFPVVIEKGYELPDSGWWGHYLTIYGYDDGLQELYSQDS
                     YLGPFDGSGRVDPYDEFLHFWQQFSNTFYVVYRPEQEETVKAILGEDMYDDFKMWQKV
                     AAMAETETLERPDDVFSWFNLGTALTRMGGLTGENQYYQGGAQAFDKAREIGLPPRML
                     WYQFQPYLAYLKLDRYQDVIDLADATLETQGGRNVEETFWYKGHALAYLGDIAGAIEA
                     YQTALTVNKNFYPAQWSLDSLQGTGG"
BASE COUNT          529 a         1024 c          834 g          485 t
ORIGIN      
        1 cccggctcaa aggacggcaa aggccgtcga gaagcgcatg cgaaaataca ggctgcctct
       61 gctcctcggc ctgctgctgg caggtattat aactgtgcga ttcctgccgg ctatgttgcg
      121 gacgatgccg acgcgctacg ccatgcggct gcccgcgccg ctgcaagccc tggccctgcc
      181 ggccgcttcc acgcccattt tgcccacagc cgccgcgccg cgcgatgccg ctgccctttt
      241 gggcaacgcc gccgttcccg cgacagccac gccgccgccc agccccacgc cgcaaaccgt
      301 ggccgcgggc tttgccacgg ccgcgccggc gaccagcccg gcaacgccca cgctggcacc
      361 cacgccaacg gccacgccct ggcctgtgcc cagcgccgcg cgcatgactg gtttcaccca
      421 cgtctttcag gaatggaaca actgtggccc ggccacgctg gtcatgaccc tgactcattt
      481 cggccgcgtc ctcagccagc gcgacaccgc cctcgtcttg cgccctgacc ccgaagatcg
      541 caacgtcacc ccgcacgaga tggccgactt cgtcaacaac acccaggacg gcctccacgc
      601 cgtcgctcgt atcaacggcg acctgaggct gctcaagcag ctactggcca ataacgtgcc
      661 ggtgatcatc gagatcggct tcgacccacc gggcgagtac cagtggttgg gctggtatgg
      721 ccactatctg ctgcctgtcg cctatgacga agcggccggg cagttctggg tctacgactc
      781 ctggctgggc accagcgaag tgccgcagac caacgccgac gccaacggtc gcgtcctgac
      841 ggaggccgaa ctagatgcct tctggacgga gttcaatcac tcctacattg ttgtctatga
      901 aacggcgcag gagacgcttg tccaacagct tctcggcgac acgctgaacg acgacgccat
      961 gtgggcggcc agcctggcgc gggcgcagac cgacgccgcg cgcgccccgg acaacgcctt
     1021 ctactggttt aacctcggct ccacctacaa cgccctcggc cgctacgatg aggccgccgt
     1081 ggcctttgat gaagcccgcg ccatcggctt gccctggcgc atgttgtggt accagttcgg
     1141 cccctacgaa gcttactacc gtgtcggccg ctatgacgat gtcattctgc tgacacagac
     1201 gacgctccag gaccgcccct acttcgagga gtcctactac tacctgggcc tggcgcaggc
     1261 ggcgctgggc gattccgcgg ccgcgcgcga aaacttacaa aaggcccaca ccctcaaccc
     1321 gagcttcgcg ccggccgaaa aggcgctggc cgaattgccg gccgatggct aacctcccat
     1381 ccgcgctggc cgagcaagag gactaatttt atgcgtaatc gaaccaaaag catcgtactt
     1441 ctgatcgctg ttgcccaact cggccttatc attggactgc tggctctgcc gacggtcgtc
     1501 caggccatcc ccggccgcta ccgcgtctgg ctccaggaaa atcatccggc cctcggccgt
     1561 gtctccgaag gggtcatcca ccaggtggct cccgtggcca cagctttgcc cgcgccacaa
     1621 caggtggctg agagcgccat cgacattggc tcgctcatcg ccgcgcagcc aagcacgacg
     1681 ttggccagcg ccccggacga gacggagcta atcggcgtgc ccaacggcgc ggcgtcgccg
     1741 gccgcgccgg cggtgacggt cgctgttgag ccaacggccg cgcccaccga accagcacta
     1801 acacccactc cggcggccac cgccaccccc gccccaaccc ccacgccgat gcccttgccc
     1861 gatcgggccg ttctggaggg catgagcatt gtcaagcagt cgttcaataa ctgtggcccg
     1921 gccaacctga cccaggtcct caactggtac gactcaccca tcactcagga tgacgtcgcc
     1981 tcctacctga agcccaatcc cgaagaccgc aacgtcagtc cctggcagat tgctgattat
     2041 gtcaacgagc agatgcccgg cttcaaagcg attgcccgca gcggcggcga tctcgacatg
     2101 ctgcggcgct tcctggcgtc gggcttcccc gtcgtcatcg agaagggcta tgagttgccc
     2161 gattccggct ggtggggcca ctacctgacc atctacggct acgacgatgg cctgcaagag
     2221 ttgtacagcc aggattccta tctgggcccg ttcgatggca gcggccgcgt tgatccttac
     2281 gacgaattcc tgcacttctg gcagcagttc agcaacacct tttacgtcgt ctaccgccct
     2341 gagcaggaag agacggtcaa ggccatcctt ggcgaggaca tgtacgacga ctttaagatg
     2401 tggcagaagg ttgccgccat ggccgagacg gagacgctgg agcgaccgga cgacgtcttc
     2461 tcctggttca acctgggcac ggccctgacg cgcatgggcg gcctgacagg cgagaaccaa
     2521 tactaccagg gtggcgcaca agccttcgac aaggcccgcg agatcggtct gccgccgcgc
     2581 atgttgtggt accagttcca accctatctg gcctacttga agctggatcg ctaccaggac
     2641 gtcatcgacc tggccgacgc gacgctggag acgcagggcg gccgcaacgt cgaggagacg
     2701 ttctggtaca agggccatgc cctggcctac ctgggcgaca tcgccggggc catcgaggcc
     2761 tatcagacgg cgctgaccgt caacaagaac ttttaccccg cgcagtggtc gctcgactcg
     2821 ctgcaaggca ctggcggcta agggccgccc ccgccagcga cgacagcctg ta
//
LOCUS       DAOSMQ010000412         2871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_34794_length_2871_cov_4.908026, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000412 DAOSMQ010000000
VERSION     DAOSMQ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34794_length_2871_cov_4.908026"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /gene="pepT"
                     /locus_tag="PK829_12060"
     CDS             <1..1027
                     /gene="pepT"
                     /locus_tag="PK829_12060"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010140454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOU41990.1"
                     /translation="TIALLAHVDTSPAFSGAGIKPIVHRHYQGGPIPLPDDPEQALTY
                     ENSPYLKDRIGDDIITASGTTLLGADDKAGVAIIMAAAGYLLKHPEIPHGPIRIGFTP
                     DEEIGRGVHADLPADLGAKFGYTLDGAHRGELVYETFSADRAVVRVQGVSIHPGWAKD
                     KLVNALHLAAKIVDTMPQVTLTPETTEGRQGFIHLYAMSGTAAAAELHFILRDFEMAE
                     MERQGALIQQVCATIAATEPRAHITCEVIPQYRNMRYWLENDMRPVDMAVKAYAAAGI
                     EHYFYPIRGGTDGSRLTEKGVPTPNLFTGMQNIHGPLEWVSVQDMALAAEMCVQLAQT
                     WTRATNG"
     gene            1061..2761
                     /locus_tag="PK829_12065"
     CDS             1061..2761
                     /locus_tag="PK829_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004715132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU41991.1"
                     /translation="MSKQLATAKPAAVAQSESWLPMAILVLAQVQMAFNVNAIPVSMG
                     PIVEQLGIPATSVGTALVVYSLFVAAFVMVGSKLGKMFGARLVFQLTALAHGVAMGIM
                     ALSRDANGMNLAQALAGLAAAALVPTLVVLIAANYHGKQQAQALGILAATPAISGALA
                     FFIAGFLGTALSWRYSFAILFFVSLVVFLLSFRLKPIPRQQGIRIDTMGAGMAAIGII
                     LLSLGFNNLNNWGMLMAKPAAPFNLVGLSPAPIMILGGVVLLQAFFAWSHMRAQVGHM
                     PLLSLEVLDSTYERSAVLALLVIGALGPAINFLIPLYIQIVQGQTTLFTAVAVVPYTL
                     SIALSAIFIVRFYQRLSPRMIAIAGFVMVAVGLVILSFTVRNDWGTPLVILALIIVGL
                     GEGSLLTLLFNIMVTSSPKELAGDVGALRGVANNLSTAVGTAFAGVVAVGLLSLIVTT
                     AIGQSNLPRSLQRQVNLDQVNFISNTQLETILGETTATDEQVATAVAINEDARVRALR
                     ASFLILAGLALLAIVPSFGLPNYTPGEIPPEGALPATPAGRRQTPESRAQLAARRRQR
                     "
BASE COUNT          462 a         1004 c          901 g          504 t
ORIGIN      
        1 gaccatcgcc ctgctggccc acgtggacac ctcgccggca ttcagcggcg cgggcatcaa
       61 gcccatcgtc catcgccact accagggcgg ccccattccc ctgcccgacg atccggagca
      121 ggcgctcacc tatgagaatt cgccctacct gaaggatcgc atcggcgacg acatcatcac
      181 cgccagcggc acaacgctgt tgggcgctga cgacaaggcg ggcgtcgcca tcatcatggc
      241 cgctgcgggc tacctgctga aacaccccga aatcccccac ggccccatcc gcatcggctt
      301 cacgcccgac gaggagatcg gccgcggcgt ccacgccgat ctgccggccg acctgggagc
      361 caagttcggc tacacgctcg acggcgcgca tcgcggcgaa ctggtctacg agacattctc
      421 ggccgaccgg gctgtcgtcc gtgtccaggg cgtgtcgatc catcccggct gggccaagga
      481 caagctggtc aacgcgctgc acctggcggc caagatcgtc gataccatgc cccaggtgac
      541 gttgacgccg gagacaaccg aggggcggca ggggttcatc cacctctacg ccatgagcgg
      601 cacggccgcg gcggccgaac tgcacttcat cctgcgcgac ttcgagatgg ctgagatgga
      661 gcgccaggga gcgctgatcc agcaggtctg cgccactatc gccgccaccg agccacgcgc
      721 ccacatcacc tgtgaggtca tcccgcagta tcgcaacatg cgctactggc tggagaacga
      781 catgcgcccg gtggacatgg ctgtgaaagc ctacgccgcg gcgggcatcg aacattactt
      841 ctaccccatt cgcgggggca cggacggctc gcgcctgacc gagaagggcg tgccgacgcc
      901 caacctcttc accggtatgc agaacatcca cggcccactg gagtgggtca gcgtccagga
      961 catggccctc gcggcggaga tgtgcgtcca gttggcgcag acgtggacga gagcgacgaa
     1021 cggctagacc cgcgccgctc cgggagcatg gacgaaaaca atgagcaaac aacttgctac
     1081 cgccaaaccg gcggccgttg cccagagcga atcgtggctg ccgatggcca tcctcgtcct
     1141 ggcccaggtg caaatggcgt tcaacgtcaa cgccattccc gtctccatgg ggccgatcgt
     1201 ggagcaactc ggcattcccg ccaccagcgt gggcacggcg ctggtcgtct actcgctgtt
     1261 cgtggccgcc ttcgtcatgg tcggctccaa actcggcaag atgttcggcg cgcggctggt
     1321 gttccagctg acggcgctgg cccacggcgt ggccatgggc atcatggccc tcagccgcga
     1381 tgccaacggc atgaacctgg cccaggcgtt ggccgggctg gccgcggcgg cgcttgtgcc
     1441 gacgctggtc gtgctcatcg ccgccaacta tcacggcaag caacaggccc aggcgctggg
     1501 catcctggcg gccacgccgg ccatctccgg cgcgttggcc ttcttcatcg ccggcttcct
     1561 gggcacggcg ctgagttggc gctactcctt cgccattttg ttcttcgtgt cgttggtggt
     1621 cttcctgctc agcttccgcc tgaagcccat tccgcggcag caggggattc ggatcgacac
     1681 catgggcgcg ggcatggccg ccatcggcat catcctgctg agtctgggct tcaacaatct
     1741 caataactgg ggcatgttga tggccaagcc ggcagcgccg ttcaacctgg tggggctgtc
     1801 gccggcgccg atcatgatcc tcggcggcgt ggtcttgctg caagcgttct tcgcctggtc
     1861 gcatatgcgc gcgcaggtcg gccacatgcc cttgctgtcg ctggaggtgc tcgattcgac
     1921 ctatgagcgc tcggccgtcc tcgccctcct ggtcatcggc gctctggggc cggcgatcaa
     1981 cttcctgatc ccgctctaca tccagatcgt ccaggggcag acgaccctgt tcacggccgt
     2041 ggcggttgtg ccctacacgc tgtcaattgc cctgtcggcc atcttcattg tccggttcta
     2101 ccagcgcctg tcgccgcgca tgatcgccat cgccggcttc gtgatggtgg ccgtcgggct
     2161 ggtcatcctg tccttcactg tccgcaacga ctggggcacg ccgctagtca tcctggccct
     2221 catcatcgtc ggcctgggcg aaggctcgct gctgacgctg ctgttcaata tcatggtcac
     2281 atcgtcgccc aaggagttgg ccggcgatgt gggcgcgctg cgcggggtgg ccaacaacct
     2341 ctccacggcc gtcggcacgg ccttcgccgg cgttgtcgcc gtcggcttgc tgagtctgat
     2401 cgtcaccacg gctatcggcc agtcgaacct gccgcgctcg ctgcaacggc aggtcaatct
     2461 cgaccaggtc aacttcatca gcaataccca actggaaacc attctgggcg agacgacggc
     2521 caccgacgag caggtggcca cggccgtggc catcaacgaa gacgcccgcg tgcgggcgct
     2581 gcgggcgtcg tttctgatcc tggccgggct ggcgttgctg gccattgtgc cgtcttttgg
     2641 cctgcccaac tacacaccgg gcgagattcc gccggagggc gccttgccgg ccaccccggc
     2701 cggccggcgc cagacgcccg aaagccgggc gcagttggcc gcgcgccggc ggcagcgcta
     2761 gtcgtcgcca atgggcctgt ttgcgtcgcg gcgctggccg cgacgcgaac agcccagcta
     2821 ccggccagac cttacctcac accgcggcgg cggcggaagc aaacaggttc a
//
LOCUS       DAOSMQ010000413         2869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_34824_length_2869_cov_4.199716, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000413 DAOSMQ010000000
VERSION     DAOSMQ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34824_length_2869_cov_4.199716"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PK829_12070"
     CDS             <1..1241
                     /locus_tag="PK829_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41992.1"
                     /translation="WDGDLDDFVQLQRVAYLAAKEANPDAAVHLGAFTYFWDPGYFAR
                     FLDAVAADPEAAANGYYFDAATAHLYFQPNAVYNVLYAFRKVMADHGLDKPLWLVETN
                     APPMDDPYWVVPNWTLAVSLNEQAAFIPQALAAAISAGAERIAVYKLRDTEADHAANP
                     EPFGLVRWDDSRRPAYNTYRVAIRLMAGATAGERERWDAVGQVRLEQPEQTTTVLFAR
                     LPGQQTARVPATADTARWIDMWGKEEEIAAEDGVFTVELPGALCLQTIADYCMIGGTT
                     YYLVQANEALRARQLAATRAAAEATATAPAATRTATPRATPTASRTPRPTATAVATAT
                     APATATAPATVTATAPSPTATVTPAPVATMTLASMPAPMDSARSATSGGLSGYVGFVV
                     IGVGVLLGLGLVGWRLAGRR"
     gene            complement(1301..1615)
                     /locus_tag="PK829_12075"
     CDS             complement(1301..1615)
                     /locus_tag="PK829_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOU41993.1"
                     /translation="MLGLQTPYTNNGGTSKPVNIIKVSARSRTAAVAGAIAGVMRESQ
                     HAEVQAIGAGAVNQAVKAIVIAKGYLAEEGVHITCTPSFVDVDIDAQERTAIRFVIEP
                     NG"
     gene            1761..>2869
                     /locus_tag="PK829_12080"
     CDS             1761..>2869
                     /locus_tag="PK829_12080"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOU41994.1"
                     /translation="MNGHQRDDLPPTASGRGVVFRAAGLVAALALLSRVLGLVREIVV
                     RQYLGVTTLEATAFDIASRFPEAIFLIVAGGAIGSAFIPTFSAYLSRDDEAGSWHLFS
                     AVLNLLTIVVTLVSVAVMLFAAPFVTFFYADNIAQTPALLPLTVALMRVMLLSPIIFG
                     ISGIVMATLNARQHFLLPAVAPSVYNLGIIAGGVAGAMMGGGGAVATVWGLAWGVVVG
                     ALGHLLVQLPGLRGQRARYAPVLTVRDPGVRQVLRLMAPRVLGLSFSEVNKFIILFLT
                     GTMVLGTLPALNAAFRILIMPQGILGQALGIAAFPTLAALAARAAHRDMRAILADSLR
                     LVLFLGLPATVLLALLAEPYVTILFERGLFDAEATA"
BASE COUNT          423 a          986 c          919 g          541 t
ORIGIN      
        1 cctgggacgg cgacctggac gacttcgtcc aattgcagcg cgtggcctac ctggccgcta
       61 aagaagccaa ccccgacgcc gccgttcacc tgggcgcatt cacctacttc tgggaccccg
      121 gctacttcgc ccgcttcctg gacgccgttg ccgccgatcc ggaggccgcc gccaacggct
      181 actactttga cgcagctacg gcccacctct atttccagcc caacgccgtc tacaacgttc
      241 tctatgcctt ccgcaaggtg atggccgacc acggcctgga caagcccctc tggctggtgg
      301 agaccaacgc gccgccgatg gacgaccctt actgggttgt gcccaactgg acgctggccg
      361 tgtcgctcaa tgagcaggcg gctttcattc cccaggcgtt ggccgcggcg attagcgccg
      421 gggcggaacg tatcgccgtc tacaagctga gagacaccga ggccgaccac gcggccaacc
      481 cggagccgtt cggactggtg cgctgggacg acagccgtcg cccggcctac aacacctacc
      541 gcgtggccat ccgcctgatg gccggagcga cggcgggcga gcgcgagcgc tgggacgccg
      601 tcggccaggt gcgcctggag cagccggagc agacgacgac ggtgctcttt gcccgcctgc
      661 cgggccagca gacggcgcgc gtgccggcca cggccgacac agcccgctgg atcgacatgt
      721 ggggcaagga ggaggagatc gctgccgagg acggcgtttt cacggttgag ttgcccggcg
      781 cactatgcct gcaaacgatt gccgactact gcatgatcgg cggaacgact tactacctgg
      841 tgcaggccaa cgaagcgctt cgggcgaggc agttggcggc cacacgcgcc gcggccgagg
      901 ccaccgccac cgcgccggcg gcgacacgca ccgccacccc acgggccacg ccaaccgcct
      961 ctcgcacgcc gcgcccgacg gcaacggctg tggcgaccgc caccgcgccg gcgaccgcca
     1021 ccgcgccggc cacggtcacc gcgaccgcgc catcgcccac cgcgaccgtg acgccggcac
     1081 cagtggcaac gatgacgctc gcgtcgatgc cggcacccat ggacagcgcg cgctcggcca
     1141 cgtccggcgg actgtcgggc tacgtcggct ttgtggtgat tggggtgggg gtgttgttgg
     1201 ggctggggct ggtcggttgg cggctggcgg gccgccgcta ggcaacaaaa aaggggcgca
     1261 gccagccggc tgcgcccctt atacatcctc gttcgcgtgc ttaaccattc ggctcgatga
     1321 cgaagcgaat cgccgttcgt tcctgcgcgt cgatgtcgac atcgacaaat gacggcgtgc
     1381 aagtgatgtg gacgccctct tcggccagat agcccttggc gatgacgatg gctttgacgg
     1441 cctgattaac agcgccggcg ccaatggcct gcacctcggc atgctgtgac tcgcgcataa
     1501 caccggcgat cgcgccagcg acggctgccg tgcgagagcg ggccgatacc ttgatgatat
     1561 tcacgggttt cgatgtacct ccattgttcg tgtacggcgt ttgcagcccc aacatgttgc
     1621 ccatcgtatt tactccattg tatgttaatc taccgtgatc attagcatac cattgaaccg
     1681 tccttgactt ttaggtattt gtatctgaat ttgttcagtc gaatgacggc ttgttgtctg
     1741 aggagcgcga tcgattgact gtgaatggcc accagcgcga cgatttgccg ccgaccgcca
     1801 gcggccgtgg cgttgtcttt cgcgccgccg gcctggtggc tgcgctggca ttgctgagcc
     1861 gtgtcctggg gctggtgcgc gagatcgtcg tgcgccagta tttgggcgtc acaacgttgg
     1921 aggcgaccgc cttcgacatc gccagccgct ttccggaagc tatcttcctc attgtcgccg
     1981 gcggggccat tggctcggcc ttcatcccca cgttctccgc ttatctgagc cgcgacgacg
     2041 aggcgggcag ctggcacttg ttctcagccg ttctcaacct gctcaccatc gtcgtgacgc
     2101 tcgtctctgt cgcggtgatg ctcttcgccg cgccattcgt caccttcttc tacgccgaca
     2161 acatcgccca gacgccggcc ctgctgcccc tgaccgtggc cctgatgcga gtcatgctgt
     2221 tgtcgccgat catctttggc atcagtggca tcgtcatggc cacgctcaac gcccggcagc
     2281 actttctcct gccggccgtg gccccgtcag tctacaacct gggcatcatc gccggcggcg
     2341 tggccggggc gatgatgggc ggaggtgggg cggtggccac ggtctggggc ttggcctggg
     2401 gtgtggtcgt gggcgcgctg gggcatctgc tggtgcagct gcccggccta cgcggccaac
     2461 gcgcgcgcta tgctccggtg ctcaccgtgc gcgaccccgg tgtgcggcag gtgttgcgcc
     2521 tgatggcccc gcgcgtgctg gggctgtcct tcagcgaggt caataagttc atcatcctgt
     2581 ttctgaccgg cacgatggtt ctgggcacgc tgccggccct gaacgccgct ttccgcattc
     2641 tgatcatgcc tcagggcatc ctcggccagg cgctgggcat cgccgccttc ccgacgctgg
     2701 ccgcgctggc cgcccgcgcc gcccaccgcg acatgcgcgc catcctggcc gactccctgc
     2761 ggctggtgct cttcctgggg ctgccggcta cggtgctgtt ggcgctgctg gccgaaccct
     2821 acgtgacaat ccttttcgag cgcggcctgt tcgacgccga ggcgacggc
//
LOCUS       DAOSMQ010000414         2865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_34888_length_2865_cov_4.877936, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000414 DAOSMQ010000000
VERSION     DAOSMQ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34888_length_2865_cov_4.877936"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PK829_12085"
     CDS             complement(<1..1088)
                     /locus_tag="PK829_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOU41995.1"
                     /translation="MNDAQHSLRKILPAALLALLVALALYPAVGWAQDATPTATTAPA
                     EATPGDPPLNITSVQPGTVVNTGQTEIVITGSGFVDGAVVVLSNWGGLETTFVSRQVL
                     RANVPAGVPPGTYDVQVVKPNAATTVLRGGLTVILPAGPTDTPEPSPTPAPTDFIRPL
                     LVVQSYGASAPQITPGQNLDFEMTIVNAGQAAAANVLATFVSGDFVPRATGGVRALGT
                     LAPGQSIRFWQPLFATDDLRGKDQAVLKVTATYTDINGQSYESSFELTFPVVPQASGG
                     AAATATPTATPTGTPGPRLRPQLLVTGYTTDPEQLQPGVPFALTMNVSNEGEANARNV
                     TLIVGGGSAGSGSADGTPEAGGVSGAGGS"
     gene            complement(1093..1869)
                     /locus_tag="PK829_12090"
     CDS             complement(1093..1869)
                     /locus_tag="PK829_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU41996.1"
                     /translation="MIAPQAGVPDAPFIAISGLRKSYALGGVVVHALDGVDLGIRAGT
                     FHTIMGPSGSGKSTLLYLLGGLDRPTAGRIVVAGAALQEMDENALAAYRREVVGFIFQ
                     SFNLISSLSAWENVAFPMRFAGVSRRARRERAFALLERVGLADRRQHRPSELSGGQQQ
                     RVAIARSLVNEPRLVLADEPTGNLDTASGQSIMELLAELHSDGRTIVVVTHDPRMAAY
                     ATHQVFLLDGRTVSDAEYHAVTGMPGGSIARPVAAPASVS"
     gene            complement(1881..2669)
                     /locus_tag="PK829_12095"
     CDS             complement(1881..2669)
                     /locus_tag="PK829_12095"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOU41997.1"
                     /translation="MTAEDELPLVQPRDNRPWRFDRVVPLLIRPRQTLADIVANDRPQ
                     WRAPIALLLLATVIHALVAGSIKAAASAAGEIILPPNFEFYTPEQQAQFQQAATATNN
                     PTFNYILPALGAALSALLLWLLVSWLLHLVLTLLGGRGTSQGAINVVAWASLPFVLRA
                     VVQIVAMLMTDQLVGGSGLAGFAPAGDGSLRVLLASLLGQIDLYTIWHILLVYIGARL
                     SSQLPRGKCWLAVLLVFALVMAVRALPDVVLARFGDLTIIQPFL"
BASE COUNT          465 a          920 c         1067 g          413 t
ORIGIN      
        1 gagccgcccg cgccggagac gccgcccgct tccggcgtgc cgtcggcgct gccggagccg
       61 gcgctgccgc cgccgacgat gagggtgacg ttgcgggcgt tggcttcgcc ctcgttgctg
      121 acgttcatcg tcagggcgaa gggcacgccg ggctggagtt gttccgggtc ggtggtgtag
      181 ccggtgacga gcagttgcgg gcgcagccgg gggccgggcg tgcccgtggg cgtggccgtc
      241 ggcgtcgctg tggctgccgc gccgccgctg gcctggggca cgacggggaa ggtcagttca
      301 aatgacgact catagctctg gccgttgatg tcggtgtagg tcgccgtcac cttgagcacg
      361 gcctgatcct tgccgcgcag gtcgtcggtg gcgaagagcg gctgccagaa gcggatcgac
      421 tgcccgggcg cgagcgtgcc cagggcgcgg acgccgccgg tggcgcgggg cacgaagtcg
      481 ccgctgacga aggtggccaa gacgttggcg gccgcggcct gcccggcatt gacgatggtc
      541 atctcgaaat cgaggttctg gccgggggta atctgcggcg cgctggcccc gtaggactgc
      601 acgaccagca gcggccggat aaagtcggtc ggcgcgggcg tgggcgaggg ttcgggcgta
      661 tccgttggcc cggccggcag gatgacagtc agcccgccgc gcagcacggt cgtggccgcg
      721 ttgggcttga ccacctggac atcataagtc cccggcggta cgccggccgg gacgttggcg
      781 cgcagcacct ggcggctgac gaacgtcgtc tccagcccgc cccaattgga caggacgacg
      841 accgccccat cgacgaaacc ggagccggtg atgacgatct cggtctggcc ggtgttgaca
      901 accgtgcccg gctggacaga ggtgatgttc agcggcgggt cgccgggtgt cgcttcggcc
      961 ggggccgttg tcgccgtggg cgtggcgtcc tgtgcccagc cgacagccgg gtacagcgcc
     1021 agagccacca gcaaggccag cagcgccgcc ggcaggattt ttcttagact gtgctgcgcg
     1081 tcattcataa gttcaactca cactcgcggg cgcggccacc ggccgcgcga tggagccgcc
     1141 cggcataccg gtgacggcgt ggtactcggc gtcgctcacc gtccggccgt cgagcaggaa
     1201 gacctggtgg gtggcgtagg cggccatgcg cggatcgtgg gtgacgacga cgatggtgcg
     1261 gccgtcgctg tgcagctcgg ccagcagttc catgatactc tgcccgctgg ccgtgtcgag
     1321 attgccggtc ggttcgtcgg ccagcaccaa tcgcggctcg ttgaccagcg agcgggcaat
     1381 ggccacgcgc tgctgctggc cgccagacag ctccgacggg cggtgctgtc gccggtcggc
     1441 caggccaacg cgctccaaca gggcgaaggc ccgttcgcgc cgcgcgcgcc gggagacgcc
     1501 ggcgaagcgc atcggaaagg cgacgttctc ccaggcgctg aggctggaaa tgaggttgaa
     1561 cgactggaag atgaagccga cgacctcgcg gcgataggcg gccagggcgt tctcatccat
     1621 ctcttgcagc gccgccccgg cgacgacgat gcggccggcc gtcggccgat ccagcccgcc
     1681 caacaggtag agcagcgtgc tcttgccgga gccggacggc cccatgatgg tgtggaacgt
     1741 gcccgcgcga atgcccagat cgacgccgtc gagggcatgg acgaccacgc cgcccagcgc
     1801 gtaggatttg cgcaggccgc tgatggcgat gaagggcgcg tcgggcacgc cggcttgcgg
     1861 ggcgatcata ggcggcgcgg ctagaggaag ggctgaatga tggtcaggtc gccgaaacga
     1921 gccaggacca catcgggcag ggcgcgcacg gccatgacga gggcgaagac cagcagcacc
     1981 gccagccagc acttgccacg cggcaactgg ctgctgagcc gcgcgccgat gtagacgagg
     2041 aggatgtgcc agatggtata cagatcgatc tgccccagca gcgaggccag aaggacgcgc
     2101 aacgagccat cgccggccgg ggcgaagccg gccaggccgc tgccgccaac cagctggtcg
     2161 gtcatcagca tggccacgat ctgcaccacg gcccgcagga cgaacggcag gctggcccag
     2221 gcgacgacgt tgatggcccc ctggctggtg ccgcgcccgc ccagcagcgt cagcaccagg
     2281 tgcagcagcc agctcaccag cagccacaac agcagcgcgc tcagcgccgc ccccaacgcg
     2341 ggcaggatgt agttgaaggt cgggttgttg gtggccgtgg ccgcctgctg gaattgggcc
     2401 tgctgctccg gtgtgtagaa ctcgaagttg ggtggcagga tgatctcgcc ggcggcgctg
     2461 gcggcggcct tgatgctgcc ggcgaccagg gcgtggatca ccgtggccag cagcagcagg
     2521 gcgatgggcg cgcgccactg cggccggtcg ttggcgacga tgtcggccaa cgtctggcgc
     2581 ggccggatga gcagcggcac cacgcggtcg aagcgccacg gccggttgtc gcgcggttga
     2641 acgagcggta gttcatcttc agcggtcata gggcctgtcc ggcggccgcg catgagatag
     2701 ccggcgcgac cgccccggta ctatagccga tgacccgccg ccgtgccacg cgccccaagc
     2761 aaacgcacat ggagggggtg gacagtggac agtagacagt agacagtagg cactcaagtt
     2821 gctaccttga gcttaacaag aagaagtcca tgaactattc tcagg
//
LOCUS       DAOSMQ010000415         2862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_34948_length_2862_cov_3.797649, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000415 DAOSMQ010000000
VERSION     DAOSMQ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34948_length_2862_cov_3.797649"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK829_12100"
     CDS             complement(<1..285)
                     /locus_tag="PK829_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41998.1"
                     /translation="MDVFLDIIRHIRESIGTEILAPIITDATYRRGYFAGIMTVVVFG
                     VFVWLRIQLLMALAKIRAFFQPSALPATRPGPSGYDRALGCRDGFLRPGCI"
     gene            complement(501..2249)
                     /locus_tag="PK829_12105"
     CDS             complement(501..2249)
                     /locus_tag="PK829_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU41999.1"
                     /translation="MRKTPLLSLLAIFVLLVAGATALPARAQTGPNLLENPGFENGHY
                     NQDGIPQITVPNGWRMYWSNEELIFGGEWVTTRPETVVWNIKGAPPGEEFYWKDGAYT
                     MKVFNTWAPLWAALAQDVEGLQVGAKYRLSVPVYIDVFADYEGGQKIKPERKDMAKVR
                     LGAGPVGATWRDENAIAYSGWWTGDSIPEFYQTYPLFVYDFTATQPNMTVYIEMASAY
                     PFPHNGFFFDTPSLVSLGAAPAAAAPAANPAAGQSAASQPAAPVQAQPAATIVPPTPR
                     PDGSIVHTVVAGDTLWVIAIQYAEVLGLAPLDALASIRELNNDPTFINPGDEILIKAG
                     TGETASATPGEGTAAATDAAAGTPAATVEGEVGTPSADATAAGDATPAATAAAGANAD
                     TGTPAAGDTNAASDTATPAAVAGTICVGTFLDANADGQRNEGEELVANAAVAISRGGT
                     TASTYITDGVSEPYCFELTDADSYQLQLYPPAGLAATTEDNWAVSIANGESYTVLFGL
                     GNAPATTPDTAADAATQLTTGDQAATDTTGGTVDNATTADDGGLFSGRLGVIVLGVAG
                     LLLVLAALGVYLLRRG"
     gene            complement(2523..>2862)
                     /locus_tag="PK829_12110"
     CDS             complement(2523..>2862)
                     /locus_tag="PK829_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42000.1"
                     /translation="TISPSGTTRAAAALVVYGLFFLSLARQMLHIATSYNYTLDLTQL
                     ALPLARPENTALLLRAVFPNAASWPWLLPLVLVAAVVAVFDRKGSGARGQGPGEKAHA
                     KGPRRKGREE"
BASE COUNT          517 a          892 c          956 g          497 t
ORIGIN      
        1 gatgcatcct ggccgcaaga acccgtcgcg acagcccagg gcgcgatcgt agccgctggg
       61 gccggggcgc gtggccggca gggccgatgg ctggaagaag gctcttatct tggccagggc
      121 catcagcaat tgaatccgca gccatacaaa cacgccaaag acaacaacgg tcataatgcc
      181 cgcgaagtac ccgcgacgat aggtggcatc ggtaatgatc ggcgcgagga tttctgtgcc
      241 gatcgactcg cggatatggc gaatgatgtc cagaaagaca tccattcctg cccccactct
      301 tcgcttgacc tgccgactac gatttggaac ttctcctcat tgtagcataa gctctgcatc
      361 aatagtgcca tcgagcgaga caattgccat gtcaattgat gcaatttggc gcacacgtgg
      421 gtgctcgctc aggacggagg catgaaaaag aggggcttgc ggcccctctc ctgatcatcg
      481 attgctgcta acgccgtggc ttatccccgc cgcaacaggt aaacccccag cgctgccaac
      541 accagcaaaa gcccagccac ccccaggaca atgacgccca gccggccgct gaacagcccg
      601 ccatcgtcag ccgtcgtagc gttatcgact gtgccaccgg tggtgtcggt ggccgcctgg
      661 tcgccggtcg tcaactgcgt ggcggcgtcg gcggccgtgt cgggcgtggt cgccggcgcg
      721 ttgcctaggc cgaacaagac cgtgtaggac tcgccgttgg cgatggacac ggcccagtta
      781 tcctcggtcg tcgccgccag acccgcgggc ggatagagtt gcagctggta ggagtcggcg
      841 tcggtcagct cgaagcagta cggttcgctg acgccgtcag taatataggt cgaggcggtc
      901 gtgccgccgc gggaaatggc tacggcggcg ttggctacta gctcttcgcc ctcattgcgt
      961 tggccgtcgg cgttggcgtc aaggaatgtg cccacgcaga tagttccggc cacggcggca
     1021 ggcgtggcgg tgtcactggc ggcgttggtg tcgcccgccg ccggcgtacc cgtatcggcg
     1081 tttgctcccg cggcggcagt ggcggccggg gtggcatcgc ccgcggcggt ggcgtcagcg
     1141 gacggcgtgc ccacctcgcc ctcgaccgtg gccgcaggag tgccggccgc cgcatcggtt
     1201 gccgcggctg taccttcgcc gggcgtggcc gaggcggtct cgcccgtgcc ggccttgatg
     1261 aggatctcat cgccggggtt gataaaggtg ggatcgttgt tcagctcgcg gatggacgcc
     1321 agcgcgtcta gcggggccag gcccagcact tcggcgtact ggatggcgat gacccacagc
     1381 gtatcgccgg ccacgacagt gtggacgatg gagccgtcgg gccggggcgt gggcggaacg
     1441 atggtggccg cgggctgggc ctgaacgggt gcggccggct ggctggcagc cgattgcccg
     1501 gcagccgggt ttgccgcggg cgcggccgcg gccggcgcgg cgcccagcga caccagcgac
     1561 ggcgtgtcga agaaaaagcc gttgtggggg aaggggtaag ccgaggccat ctcgatatag
     1621 acggtcatat tcggctgcgt ggccgtgaag tcatagacaa atagcgggta ggtctgatag
     1681 aactccggga tagagtcgcc tgtccaccag ccgctatagg caatggcgtt ctcgtcgcgc
     1741 caggtagccc ccaccggccc cgcgcccaga cgcaccttgg ccatatcctt gcgctcgggc
     1801 ttgatcttct ggccgccttc gtagtcggca aagacatcga tgtagacggg cacgctgagg
     1861 cggtacttgg cccccacttg cagcccctcg acatcctgcg ccagcgcggc ccacaggggc
     1921 gcccaggtgt tgaagacctt catcgtgtag gcgccatcct tccaatagaa ctcctcaccc
     1981 ggcggagcgc ccttgatgtt ccagacaact gtctccggcc gggtggtgac ccactcaccg
     2041 ccaaagatca gttcttcatt tgaccagtac atgcgccaac cattgggcac ggtgatctgg
     2101 gggatgccat cctggttgta gtggccgttc tcgaagccgg ggttttccag caggtttggg
     2161 ccggtttgcg cccgcgccgg gagggccgtc gcgccggcca ccaacagaac aaagatcgcc
     2221 agaagcgaca gcaggggtgt ctttcgcatg ttgaatctct tctccattcc accacatcgg
     2281 cctgtcggcg cggcagcaac ccgccaagcg tggcccaaca gccgtatggc gcgccgaggc
     2341 gcgccgctac atcaacatgt tatcttaaca gcacggccgc gggcatacca catttctggg
     2401 cggcgcatga gtggaaggtt aatagggtag aggccagagc caggggatag gggccgggtt
     2461 ccaggggagg aggctcacgc aaagccgcaa agaagcaaag acgctaagga agaaaagcaa
     2521 gctcactctt ctctgccttt gcgccttggc cccttggcat gagccttttc ccctggcccc
     2581 tggcccctgg cccctgaccc cttccggtcg aatacggcca ccaccgcagc cacaaggacg
     2641 agcggcagca gccagggcca gctggcagca ttggggaaga cggcgcgcag cagcagggcc
     2701 gtgttctctg gccgggcaag aggcagggcc aattgggtca ggtcgagggt gtagttatag
     2761 cttgtggcaa tgtggagcat ctgccgggcc agcgagagga aaaagaggcc atagacgacc
     2821 agggccgccg cggccctggt cgtcccggag ggcgatatcg tc
//
LOCUS       DAOSMQ010000416         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35044_length_2856_cov_4.762585, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000416 DAOSMQ010000000
VERSION     DAOSMQ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35044_length_2856_cov_4.762585"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PK829_12115"
     CDS             complement(<1..934)
                     /locus_tag="PK829_12115"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU42001.1"
                     /translation="MTDTSLAYARAHRERHLAELLDFLRLPSISTDPAYHEATADAAD
                     WLAGQMKDAGLENVRVFPTERHPLVYADWLHAGPGAPTVLIYGHYDVQPADPLELWTT
                     PPFEPTVREDYIYARGAADDKGQLYLHVKAVEATLRADGRLPLNVKFLIEGEEEIGSH
                     SLHAFIPQNRDLLAADSALISDSAMLGPGRPALVYGLRGNCHVLLDITGPDRDLHSGT
                     YGGSIDNPLNALGHLLARLKNESGHVMIPGFYDRVRPLSLEERELLAQFPLSEAEWLM
                     QTGAPRLWGEPEYTLVERVGARPTLDVTGMFGGYI"
     gene            1064..1585
                     /gene="scpB"
                     /locus_tag="PK829_12120"
     CDS             1064..1585
                     /gene="scpB"
                     /locus_tag="PK829_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOU42002.1"
                     /translation="MPVPADGLSPSALLESLLFVASGPVSAARLAAALELSTAAVAGL
                     LRDLAADYAERGLRLQWSDGLVQLTTAAGAGATIERFLGLEVTTRLSPAALEVLAIVA
                     YLQPATRPQIDQLRGVNSDAALRSLLSKGFVQEVGRLEKPGRPILYGTTPDFLQAFGL
                     NSIDEMPRLDEEE"
     gene            1626..2435
                     /locus_tag="PK829_12125"
     CDS             1626..2435
                     /locus_tag="PK829_12125"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU42003.1"
                     /translation="MQERLQKLMAQANLGSRRASEVIIAAGRVTVNGRIAKLGDKADP
                     AVDQVLVDGRPLFAPPRAPEGEFLYIALYKPRGVISSLEDELEAGRPTVRDLVPLAAD
                     VHIYPVGRLDKPSEGLVLMTNDGELAHRLTHPRYGHEKVYDVTVEGRMSDRILEQWRR
                     GVMLDGRITAPAPIEVLERANERTRLRVILREGRKRQIRRIAAALGHPVQRLLRERIG
                     PLSLGDLQPGEWRHLTAAEVRDLRHSAAGSPAAAPTHHPLGKEHRAPRTSG"
     gene            2413..>2856
                     /gene="cmk"
                     /locus_tag="PK829_12130"
     CDS             2413..>2856
                     /gene="cmk"
                     /locus_tag="PK829_12130"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOU42004.1"
                     /translation="MPHEHLVDVIAIDGPAASGKSTIGYALAQRLGYLFLDTGSMYRA
                     MTLAALRLGLALDDVAALTALAEQIEIDVLPPGEAADGRQCTILLNGEDVTWALRLPE
                     VEAGVSAVSAVAGVRAQLVSRQREMGRRGGIVMVGRDIGTVVMPDA"
BASE COUNT          446 a          962 c          966 g          482 t
ORIGIN      
        1 caatgtagcc gccaaacatg ccggtcacgt ccagcgtcgg ccgcgcgccg acgcgctcca
       61 ccagcgtgta ctccggctcg ccccataacc gcggcgcgcc cgtctgcatc agccactccg
      121 cctcgctgag gggaaactgg gccagcaact cgcgctcctc cagcgacagc ggccgcaccc
      181 ggtcgtagaa accggggatc atcacgtgcc cggattcatt cttcagccgg gccagaaggt
      241 ggccgagggc attgagcggg ttgtcgatgc tgccgccata ggtgcccgaa tggagatcgc
      301 ggtctgggcc ggtgatgtcc agtaacacgt ggcagttacc gcgcaggcca tagaccaggg
      361 ctggccggcc ggggccaagc atggccgagt cggagataag cgccgaatcg gccgccagca
      421 ggtcgcggtt ctgggggatg aaggcatgaa gactgtggct gccgatctct tcttcgccct
      481 cgataaggaa cttgacattt agcggcagac ggccgtcggc gcgcagagtg gcttcgaccg
      541 ccttgacgtg gaggtagagc tgccctttgt cgtcggccgc gccgcgggca tagatgtagt
      601 cctcacgcac ggtcggctcg aagggcggtg ttgtccatag ctccagcggg tcggccggct
      661 gcacgtcgta gtggccgtag atcagtactg tcggcgcgcc cggcccggcg tggagccagt
      721 cggcatacac cagcgggtgg cgctcagtcg gaaatacgcg cacattctcc agaccggcgt
      781 ctttcatctg cccggccagc cagtcggcgg cgtcggccgt ggcttcgtgg taggcaggat
      841 cggtgctgat gctgggcagg cgcaggaagt cgagtagttc ggcgagatgg cgttcacggt
      901 gggcgcgggc gtatgcaagg cttgtgtcgg tcatagtgtt tctttcatgg gcggatgtgt
      961 tatcatcggt atcatatccg cttatcgaac gcttgtcccc tctaaagcgg ggccgcgctg
     1021 ccagaagatg atagaccgac ccgaaccgct cgcgactgac ctattgcccg tacccgccga
     1081 tggtctcagc ccctcggccc tgctggagag cttgctcttc gttgccagcg ggccggtctc
     1141 ggcggcgcgg ctggcggcgg cgttagaact gtcgaccgcg gccgtcgctg gcttgttgcg
     1201 ggatttggca gcggactacg ccgagcgcgg gctgcggctc cagtggagtg atggcctggt
     1261 gcagctgacc acggccgccg gggccggcgc gaccattgag cgcttcctcg gcctggaggt
     1321 caccacgcgc ctcagcccgg ccgcgctgga agtgctggct atcgtcgcct atttgcaacc
     1381 ggcgacgcgg ccacagatcg accagttgcg cggtgtcaat tccgacgccg ccctgcgctc
     1441 gttgctcagc aagggctttg tccaggaggt tggccggctg gagaagcccg ggcgaccgat
     1501 tctctatggc accacgcccg actttctgca agcctttggc ctgaacagca ttgacgagat
     1561 gccccggctg gacgaagaag agtgacgctg ccggccccgt ctcgacgagg ctgaccacta
     1621 gaaccatgca agaacggcta caaaagctca tggcccaggc taacctgggg tcgcgtcgcg
     1681 ccagcgaggt catcattgcc gccggccgcg tgacggtcaa cggccgcatc gcgaagctgg
     1741 gcgacaaggc cgacccggcc gtagaccagg tgctggtcga tggccggccg ctctttgccc
     1801 cgccgcgcgc cccagagggc gagttcctct acatcgccct ctacaagccc cgtggcgtta
     1861 tctcctcgct ggaggacgaa ctggaggcgg gccgccccac cgtgcgcgac cttgtgcctc
     1921 tggccgccga cgtccacatc taccccgtcg gccgtctcga caagcccagt gagggcctgg
     1981 tgctgatgac caacgacggc gaactggccc accgcctgac ccacccgcgc tacggtcacg
     2041 agaaggtcta cgatgtcacc gtcgaggggc gcatgtccga ccgcatcctg gagcagtggc
     2101 ggcgcggagt catgcttgac ggtcgtatca ccgcgcccgc gcccatcgag gtcctcgagc
     2161 gtgccaacga gcgcacgcgc ctgcgcgtca tcttgcgcga ggggcgcaag cggcaaatcc
     2221 ggcgcatcgc cgccgccctc ggccaccccg tccagcggct gctgcgcgag cgcatcggcc
     2281 cgctgagcct gggcgactta cagccgggcg aatggcgtca cctgaccgcc gccgaagtac
     2341 gcgacctgcg ccacagcgct gccggaagcc ccgccgcggc tccaacgcat cacccactcg
     2401 ggaaggaaca tcgtgcccca cgaacatctg gttgacgtca tcgccatcga cggcccggct
     2461 gcgtccggca agtcgacgat tggctacgct ctcgcccagc gcctgggcta cctcttcctt
     2521 gacaccggct cgatgtaccg ggccatgacg ctggccgccc tccggcttgg tctggccctc
     2581 gacgacgtcg cggcgctgac ggcgctggcc gagcaaattg agatcgacgt gctgccgccg
     2641 ggcgaggccg cggacggtcg ccagtgtacc attctcctca atggcgagga cgtcacctgg
     2701 gcgttgcgcc tgccggaggt ggaggcgggc gtgtcggctg tctcggccgt ggccggcgtg
     2761 cgggcgcagc tggtgtcgcg ccagcgcgag atgggccgcc gcggtggcat cgtcatggtt
     2821 ggccgcgaca ttggcacggt ggtcatgccc gacgcg
//
LOCUS       DAOSMQ010000417         2845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35211_length_2845_cov_5.462366, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000417 DAOSMQ010000000
VERSION     DAOSMQ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35211_length_2845_cov_5.462366"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..700)
                     /locus_tag="PK829_12135"
     CDS             complement(50..700)
                     /locus_tag="PK829_12135"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU42005.1"
                     /translation="MQIVNFLLAAAIGYLCGSIPFGYLYVRLLKGEDLTKIGSGRTGG
                     TNSFRAAGLGVGVLTSFSDVLKGAAAVWLTTWLLGDRLGAELLPWAQAVAGVFAVVGH
                     NWSIFLRFGGGAGTGPNVGWSAAIWPWIFPIAFLMMGGMLYFVGMASVASLSMAVVTI
                     AIFAYRFAAGIDPTPAYLVGSLVAGLVVTWALRPNIVRVLNGTERLVGRRAKRSSQ"
     gene            complement(756..1181)
                     /locus_tag="PK829_12140"
     CDS             complement(756..1181)
                     /locus_tag="PK829_12140"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HOU42006.1"
                     /translation="MPTLILSRALREEMLAHAAGLWPEEACGLLAGREERAVRLYPVE
                     NSLHSPVAYEMEPVQQVRAMLALEAEGLELLAIYHSHPDGPAQPSATDIAAAYYPDAV
                     QLILSLADRQRPSLRAFTIIAGVVAEVGWQEEASGAGQI"
     gene            complement(1311..2759)
                     /locus_tag="PK829_12145"
     CDS             complement(1311..2759)
                     /locus_tag="PK829_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOU42007.1"
                     /translation="MSDYRIEKDSLGEVRVPSGAYWGAQTQRAIHNFPITGLRPYPAF
                     VWSMALIKRAAAEVNRDLGLFGDKEAAGHTISGAEMAGAIIKAADEVLAGQWADQFVV
                     DPIQAGAGTSHNMNINEVIANRANEILGFGLEAQGKPVNPNDHVNMAQSTNDTIPTAI
                     RLGALWRLDELLATLRRLAAELNNKAAEFDGIVKSGRTHLQDAVPVRLGQEFGGYAKA
                     IERDTAKIAAAAEGLRRLGIGGTATGTGLNAHPEYHARMVRTLSALSGLTLYESDNLF
                     ESMQSMADAVHFSGALRTLAGDLTRIANDLRLLASGPTTGLDEIRLPPVQPGSSIMPG
                     KVNPVLAEMLNMAMFQVQGNDLTVMLAGQAGQLELNVMMPIIAYNLFQSMDVIINAVN
                     SFIDNCLVGITANAEKATGWLAKNAILVTALNPVIGYQKGAEVAKEALATNRTVREVV
                     VEKGYVTAEEADHLLDVSALTEGGIQGVAAGG"
BASE COUNT          471 a          913 c          949 g          512 t
ORIGIN      
        1 ttctgcgtga ctggatactg cttactgctt actgcccact gtccactgtt cactgactac
       61 tgcgcttcgc ccggcggccg acgagccgct ccgtgccgtt cagcacacgg acgatgttgg
      121 gccgtagcgc ccaggtgacg actagcccgg cgacgaggct gccgacgaga taggccggcg
      181 tcgggtcgat gcccgcggcg aagcggtagg cgaagatggc gatggtcacc acggccatgc
      241 tcagcgaggc cacggaagcc atgcccacga agtagagcat tccacccatc atcaggaagg
      301 cgatggggaa aatccacggc cagatggccg ctgaccagcc gacgttcggc cccgtgcccg
      361 cgccgccgcc gaagcgcaga aagattgacc agttgtggcc gacaacggcg aagacgccgg
      421 ccactgcctg tgcccagggc agcagttccg cgccgagccg gtcgcccagc agccacgttg
      481 tcagccagac agccgccgcg cccttgagta cgtcgctaaa cgaggtcaac acgcccacgc
      541 ccaggccggc ggcgcggaag gagttggtgc cgcccgtccg gccgctgccg atcttggtca
      601 ggtcttcgcc cttaaggagg cggacgtaga gatagccgaa ggggatggaa ccacagagat
      661 agccgatagc cgcggccagg aggaagttga caatctgcat agtgcgctcg cgagtcgagt
      721 ctacacatag aacccgcctg cggccagttc gttgcttaaa tctggcccgc gccagacgct
      781 tcctcttgcc agcccacctc ggccacgacg ccagcgatga tcgtgaaggc acgcaggctc
      841 ggccgctgcc ggtcggccag cgaaaggatg agttgtacgg cgtcggggta gtaggcagcg
      901 gcgatgtcgg tggccgacgg ctgcgccggg ccgtcggggt gggagtggta gatggcaagc
      961 agctccagcc cctcggcctc cagggccagc atggcccgca cctgttgtac cggctccatc
     1021 tcgtaggcca ccggactgtg gaggctgttc tcgaccgggt agaggcgcac ggcgcgctct
     1081 tcacggccgg ccagcagccc gcaggcctct tccggccaca gcccggcggc gtgggcgagc
     1141 atttcttcgc gtagtgcgcg gctcaggatc aaggtcggca tgggtgtgga agaagctcac
     1201 gccaagccgc aaagcgagca aaggcgcaaa gaagaataag tcttcttact ttgtatcctt
     1261 tactcccttt gcgtcctttg cgtgagcggc tttgcgtgag cccctctggc ctaaccgccc
     1321 gcggcgacgc cctggatgcc gccctcggtc agggcgctta cgtcgagcag gtggtcggcc
     1381 tcttcggccg tcacgtatcc cttctccaca accacctcgc gcaccgttcg gttggtagcc
     1441 agcgcctcct tggccacttc ggcacccttc tggtagccga tgaccgggtt gagcgccgtc
     1501 accaggatgg cattcttggc cagccagccg gtggccttct ccgcgttggc ggtgatgccg
     1561 acgaggcagt tgtcgatgaa cgagttcacc gcgttgatga tcacgtccat gctctggaac
     1621 aggttgtagg cgatgatggg catcatcaca tttagttcca actggcccgc ctggccggcc
     1681 agcatgacgg tcaggtcgtt gccctgcacc tggaacatgg ccatattcag catctcggcc
     1741 agcaccgggt tgaccttgcc aggcatgatc gacgagccgg gctgcaccgg tggcaggcga
     1801 atctcgtcca ggccagtggt ggggccggag gccagcagcc gcaggtcgtt ggcgatgcgc
     1861 gtcaggtcgc cggccagggt gcgcagcgcg ccgctgaagt ggacggcgtc ggccatgctc
     1921 tgcatcgact caaagaggtt atcgctctca tagagtgtca ggccgctgag ggcgctgagc
     1981 gttcgcacca tgcgggcgtg gtactccgga tgggcgttca ggcccgtgcc ggtggccgtg
     2041 ccgccaatgc ccagccggcg cagcccctcg gccgccgcgg cgatcttggc cgtgtcgcgc
     2101 tcgatggctt tggcatagcc gccaaactcc tgccccaggc gaacgggtac ggcgtcttgc
     2161 aggtgggtgc gcccgctctt gacgatgccg tcgaactcgg ccgctttatt gttcaactcg
     2221 gctgccagtc ggcgcaacgt cgccagcagc tcgtctagcc gccacagcgc gcccaggcgg
     2281 atggccgtgg gaatggtgtc gttggtcgac tgggccatgt taacgtggtc gttggggttg
     2341 accggcttgc cctgcgcctc tagcccaaag ccgaggattt cgttggcccg gttggcgatg
     2401 acctcgttga tgttcatgtt gtggctggtg ccggccccgg cctggatggg atcgacgacg
     2461 aactggtcgg cccattggcc ggccaggact tcgtcggcgg ccttgatgat ggccccggcc
     2521 atctccgcgc cgctgatggt atgcccagcg gcctccttgt cgccaaacag gcccaggtcg
     2581 cggttgactt cggccgcggc gcgcttgatg agggccatcg accacacgaa ggcggggtag
     2641 gggcgcaggc cggtgatggg gaagttgtgg atagcgcgct gcgtctgcgc gccccagtag
     2701 gctccggacg gcacgcgcac ttcgcccaac gagtcctttt cgatgcgata gtcgctcata
     2761 gattcctccg caatgcaaaa accggattga ccaaccaggg tcacaggtca gtcaatccgg
     2821 tcaaggttcg tcctgtaggg gcggg
//
LOCUS       DAOSMQ010000418         2842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35261_length_2842_cov_4.479010, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000418 DAOSMQ010000000
VERSION     DAOSMQ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35261_length_2842_cov_4.479010"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1049)
                     /locus_tag="PK829_12150"
     CDS             complement(183..1049)
                     /locus_tag="PK829_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU42008.1"
                     /translation="MSKQATPFTDARKSLPSTLYTEEYFLTACEGYDVFLASEGAHLS
                     RRLRDAFALADVRAGMRVLDVGCGRGEILRHCMRLGIEAHGMDYAAVATQMSREVVAA
                     ETQRAAAELQRPENEQPRPPRPGVCQADAKTLPFPDNSFDRVLMFDVVEHLFPWELQQ
                     AFVDIGRVLKPGGRLVIHTAPNRWYDTYAYPWVRRVRTLLGQGDDYPKDPRAITPVNQ
                     DVHVNEQDLLSMRRALKRGGYTGIRVWLDSPPQSRVESPVLAGLRRVAFGAPPFRWFF
                     EREVFAVAEPVK"
     gene            1195..1782
                     /locus_tag="PK829_12155"
     CDS             1195..1782
                     /locus_tag="PK829_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU42009.1"
                     /translation="MNDEQVWLQKARRGDKNAFGQLIEAYQGPVYNLAYRMLGNAGEA
                     EEAAQEAFIRAYTRLDSYDPAHKFSTWLLSITSNYCIDQIRKRRAVLLSIDEPLPPHP
                     ALHSDNNKGPEAQYLAQEREALVQGMLRELPDEYRQAVVLRYWYDMSYEEIAEIQKTT
                     VSAVKSRLFRARRQLAESGMGRSLVPAEVEDEVMV"
     gene            1822..2379
                     /locus_tag="PK829_12160"
     CDS             1822..2379
                     /locus_tag="PK829_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOU42010.1"
                     /translation="MEHEERYYLMMMGALDNELPDADRDELTAHLAGCADCRREWQAL
                     LAIDTLFRQTPLLMPVVNFAERTLALLPSSRLRVRALSAVYLILLASGIVPLLLGLFL
                     VGRYAPLLEQPALVERVVTSVVNVGRVAATVVSAVLAGAGRFVVEQPAVVGLMIILAG
                     FVLLWGGVLQRLLAQPAAISVRNRG"
     gene            2420..>2842
                     /locus_tag="PK829_12165"
     CDS             2420..>2842
                     /locus_tag="PK829_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU42011.1"
                     /translation="MKRRILIPLALVLFLIVVLAPAAFAGPLDDTVIEANEVVNNDVI
                     VLDGDLTIHEGAVVNGDVVVFNGDATIDGVVSGSVTLFNGDLHAGRAAVVEGECVLLN
                     GDVRGPASLRNCTAVGSLKLPELEMPAIPAVPAVPNIPE"
BASE COUNT          488 a          870 c          991 g          493 t
ORIGIN      
        1 cagcacgccg tcgaggatgc cgatggcctc gcggccgtgg ttggcctcgt catgggtcag
       61 gccgggcggg atgctggtca gttgccagac gcgcagggcg aaggccagca gcagtatggc
      121 cgccagcact gcgcgcgcgc gggcgacgct caggccgcga ccagtccgcc ccccgcggcc
      181 ggtcacttta ccggctcggc cacggcgaag acctcgcgct cgaagaacca gcggaaaggc
      241 ggcgcgccga aggccactcg tcgcagcccg gccagcaccg gcgactcgac ccggctctgc
      301 ggcggtgaat cgagccagac gcgaatgccg gtgtagcctc cccgcttcag cgcccggcgc
      361 atgctcagca agtcctgctc gttgacgtgg acatcctgat tgaccggggt gatggcccgc
      421 gggtccttgg ggtagtcgtc gccctggccc agcagcgtgc gcacgcgccg cacccaggga
      481 taggcgtagg tgtcgtacca gcgattgggc gcggtgtgga tgaccagccg gccgcccggc
      541 ttcagcacgc ggccgatatc gacaaaggct tgctgcaact cccacgggaa gaggtgctcg
      601 acgacgtcga acatcagcac gcggtcgaag ctgttgtcgg ggaagggcag cgtcttggcg
      661 tcggcctggc agacgccggg ccggggcggg cgcggctgct cgttttcggg gcgctggagt
      721 tccgcggcgg cgcgttgtgt ttcggccgcg acgacctccc ggctcatctg cgtggccacg
      781 gcggcatagt ccatgccgtg ggcctcgatg cccaggcgca tgcagtgacg caggatttcg
      841 ccgcggccac agcccacgtc cagcacgcgc atcccggcgc gcacgtcggc cagcgcgaaa
      901 gcgtcgcgca ggcggcgcga gaggtgcgcg ccctctgagg cgaggaagac gtcgtagcct
      961 tcgcacgccg tcaggaagta ttcttccgta tagagcgttg acggcaaaga cttgcgcgcg
     1021 tcggtgaatg gagtcgcttg tttgctcatg ggtgtggttg tcgccggcta agggtttacc
     1081 gggcgtcctg tataatgcac gtcaggcatt atagcgaccg actgcgggca ggcaaacgcg
     1141 aaacccaccg ccaccttcta cgtatagggg gtgagtagct aggagattgg aacgttgaac
     1201 gacgagcagg tctggctgca aaaggccaga cgtggcgaca agaatgcgtt cgggcagttg
     1261 atcgaagcct atcaggggcc ggtctataac cttgcctacc ggatgctggg caacgccggc
     1321 gaagccgaag aagcggcgca ggaagcgttc atccgcgcct acacgcggct ggacagctac
     1381 gacccggcgc acaagttcag cacctggctg ctgtcgatca catctaacta ctgcatcgac
     1441 cagattcgca agcggcgggc ggtcttactg tccatcgacg agccgctgcc gccgcatccg
     1501 gcgttgcaca gcgataacaa caaggggcca gaggcgcaat atctggctca ggagcgcgag
     1561 gccctggtgc agggcatgtt gcgcgagtta cccgacgaat atcggcaggc ggtcgtcctg
     1621 cgctactggt acgacatgtc ctatgaggag atcgccgaga tacagaaaac cacggtcagc
     1681 gctgtcaaat cgcgcctctt tcgcgcgcgg cggcagctgg ccgaaagcgg tatggggcgg
     1741 agtctggttc ccgccgaggt tgaggacgaa gtgatggtat aagccacgcg cgggaagggg
     1801 tcttctcgcg ggagaatagc gatggagcac gaagagagat actacctgat gatgatgggc
     1861 gcgctcgaca acgagcttcc cgatgccgac cgggatgaac tgaccgccca tctggccggc
     1921 tgcgccgact gccgccgcga gtggcaggcg ctgttggcca tcgacacgct gttccggcag
     1981 acgccgctgc tgatgccggt ggtcaacttc gccgagcgca ccctggcact gttgcccagc
     2041 agccggctgc gcgtgcgggc gctaagcgcc gtctacctga tcctgctggc cagcggtatt
     2101 gtgccgctgc tgctcggcct gttcctcgtt ggccgctacg cgcccctcct ggagcagccg
     2161 gcgttggtgg agcgggtcgt gacctcggtg gttaacgtgg gccgcgtggc cgccacggtc
     2221 gttagcgctg tgctggccgg ggcgggccgc tttgtggtcg agcagccggc cgtcgtgggc
     2281 ctgatgatca ttctggccgg gtttgtcctc ctgtggggtg gcgtgctgca acggctgttg
     2341 gcgcaacccg cagccatcag tgtgcgtaat agaggataag gacgcaggag tattggaaac
     2401 aacgctgaag gagacaagca tgaagagacg tattctgatc cctctggccc tggtgctatt
     2461 tttgatcgtc gtcctggcgc cggcggcctt cgctggcccg ctcgatgaca cggttattga
     2521 ggccaacgag gtagtcaaca acgacgtcat agtcctcgat ggcgatttga ccattcatga
     2581 gggcgcggtc gtcaacggcg acgtggtcgt cttcaacggc gatgcgacca ttgacggcgt
     2641 cgttagtggc agtgtgacgc tgttcaacgg cgacctgcac gccggccggg ccgccgtcgt
     2701 cgagggcgaa tgcgtgctgc tgaacggtga cgtgcgcggc ccggcgtcac tgcggaactg
     2761 caccgccgtt ggcagtctaa agctgccgga gctggagatg ccggccatcc ccgcggttcc
     2821 agcggtgccg aacattccgg aa
//
LOCUS       DAOSMQ010000419         2836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35360_length_2836_cov_3.800431, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000419 DAOSMQ010000000
VERSION     DAOSMQ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35360_length_2836_cov_3.800431"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PK829_12170"
     CDS             complement(<1..671)
                     /locus_tag="PK829_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU42012.1"
                     /translation="MNKQHLAVMAFESARAFAGEVAMRVRRDGQWQPTTYREMGDSIR
                     RIARALLALGVQPGDRVGIMSENRPEWSLVDLGVLAVGGITVPIYATNTRQQAAYILD
                     ETEMSVIFTGSQAAYDKIQAIAAGPTTLRYMITLDDDTTIDGQASYHFGQFLALGTDA
                     ADAEIETRLAAAESSDLATIIYTSGTTGEPKGVMLTHANLLHQFKALEGEFHVGPQDR
                     SLCVLP"
     gene            848..1558
                     /locus_tag="PK829_12175"
     CDS             848..1558
                     /locus_tag="PK829_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU42013.1"
                     /translation="MDVAPGIYSLGNRQSGHVHAYLFDDGQGITVVDTLMAADAHLIL
                     TELARIGRAPTDIKHLIMTHAHRSHLGGLARLKELSGAPVYAHEWEADIISADRVAQP
                     VSSRPWRPVRTWPLQLALNLNLSKHPPVRVDEYVHEGDRIGPLTVVFAPGHSPGHLAF
                     YWPERQVLVAGDAVCTWPVFMLGWRGFMLNVRQHRASVRRLAELDSEIVCSGHGDPLA
                     SGGASRIRAALPELDRLA"
     gene            1586..2629
                     /locus_tag="PK829_12180"
     CDS             1586..2629
                     /locus_tag="PK829_12180"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOU42014.1"
                     /translation="MARIKEYWLQLETRPWDVAPWGSDRLRAEPPPKTPAPYRPTGQD
                     VFIIRRYNANWAAPDDRPLNPWDLTEPDPATTGGTLPGATLTAKVADELVVHFRNGDQ
                     RHGLPPAERVHSLHAHGVQRAQTMGGAFPLAPPDPAQGNRRGDRIMPGESFTYRWSCP
                     QRSAAGVWLYHDGGPHGAAATAAGAFGALVVLAPGEQEADAPAGPLRRAGDSSTHFAA
                     VPAPPRRADYLLVFHELPGVGLCLNGRAGLGNTPALVAGEGTRLTLRCLNVTDQPLAV
                     SLPGHRWEHGANYTDVELLPPGGGATLAILSASAEDGGGLGEWPIIGRAGGQVVMGSL
                     VTTKGGAVALLSA"
BASE COUNT          453 a          996 c          927 g          460 t
ORIGIN      
        1 ggcaacacgc acaaggagcg atcctggggg ccgacgtgga actctccctc cagcgccttg
       61 aactggtgga ggaggttggc atgggtcagc atcaccccct tcggctcacc ggttgtgccg
      121 ctggtgtaga tgatggttgc caggtcgctt gactcggctg ctgccagccg ggtctcgatt
      181 tcggcatcgg ccgcgtctgt gcccaacgcc aggaattggc cgaagtggta ggatgcctgg
      241 ccatcaatcg tcgtgtcatc atccagggtg atcatgtaac ggagcgtggt cggcccggcg
      301 gcaatggcct gtatcttgtc gtaggcggcc tggctgccgg tgaagatgac cgacatctct
      361 gtctcatcaa gaatgtaggc cgcctgttgc cgggtgttgg tggcatagat gggcacagtg
      421 atgccgccga cggccaacac gcccagatca accagcgacc actccggccg gttctccgac
      481 atgatgccga cccgatcgcc gggctgcacg cccagcgcca gcaaggcgcg ggcgatgcgg
      541 cggatgctgt cgcccatttc gcgataggta gtgggttgcc attggccgtc gcgccggacg
      601 cgcatggcca cttcaccggc aaaggcacgc gcgctctcga aggccatgac ggccaggtgc
      661 tgtttgttca tgggggtgcc ccgcccgtaa gaggatagtg cttactatcc tcccatttcc
      721 gagaaatagc cagtgattgg cgaatgtgct cagggaaaca gcaacgcatt gacaccgccc
      781 cacccgtcct ttaccatata ccttatctca gccaccgccg cccacatcgc ccccacagga
      841 gcggctcatg gatgttgcgc ccggtattta cagcttaggc aacaggcaga gcggccacgt
      901 tcacgcctac ctgttcgacg acggccaggg catcaccgtc gtcgataccc tgatggctgc
      961 cgacgcccac ctcatcctga ccgaactggc ccgcatcggc cgcgccccca ccgacatcaa
     1021 acatctcatc atgacccatg cccaccgttc ccaccttggc ggcctggcgc ggctgaagga
     1081 actgagcggc gcgccggtct acgcgcacga atgggaggcc gatattatct cagccgaccg
     1141 cgtggcccag cccgtctcca gccggccgtg gcggccggtg cgcacctggc cgctccagct
     1201 ggcgctcaac ctcaacctct ccaaacaccc gccggtgcgg gtcgatgaat atgtccacga
     1261 gggcgaccgc atcggcccac tgacggtcgt cttcgccccc ggccactcgc ccggccatct
     1321 ggccttttat tggccggagc ggcaggtgct tgtcgccggc gatgccgtgt gtacctggcc
     1381 ggtgttcatg ctcggctggc gcggctttat gctcaacgtc cggcagcaca gagcctcggt
     1441 gcggcgactg gccgagctgg acagtgaaat cgtctgcagc ggccacggcg acccgctggc
     1501 cagcggcggc gcgagccgca tccgcgctgc cctaccggag ctagatcggc tggcctagcg
     1561 cggcgagaac gaacaggaga gcaggatggc gcgcattaag gagtactggc tgcaactgga
     1621 aacccggcct tgggacgtgg ccccgtgggg cagcgaccgc ctccgcgcgg agccgccgcc
     1681 gaaaacaccc gctccctacc gcccgacggg gcaggatgtc tttatcattc gccgctacaa
     1741 cgccaactgg gccgcgcccg acgaccggcc gctgaacccc tgggacttga ccgaacccga
     1801 cccggccacg accggcggca cgctccccgg cgcgacgctg acggccaagg tggccgatga
     1861 actcgtcgtc cacttccgca acggcgacca gcgccatggc ctgcctccgg ccgagcgcgt
     1921 ccatagcctg cacgcccacg gcgtccagcg cgcccagacg atgggcggcg cgttccccct
     1981 ggctccgccc gacccggccc agggcaaccg ccgtggcgac cggatcatgc ccggcgagag
     2041 cttcacctac cgctggagct gcccgcaacg ctcggccgcg ggcgtgtggc tctatcacga
     2101 cggcgggccg cacggcgcgg cggccacggc cgcgggcgcg ttcggcgcgc tggtcgtgct
     2161 ggcccccggc gagcaggaag cggacgcccc cgccgggccg ttgcgccgcg ccggcgacag
     2221 cagcacccac ttcgccgccg tgcccgcccc gccccggcgc gccgactact tgctggtctt
     2281 ccacgaactg ccgggcgtgg gcctgtgtct gaacggccgc gccggcctgg gcaatacgcc
     2341 ggcgctggtg gccggcgaag ggacgcgcct gacgctgcgc tgcctcaacg tcaccgacca
     2401 gccgctggcc gtcagtctgc ccggccaccg ttgggagcac ggcgcgaact acaccgatgt
     2461 cgaactgttg ccgcccggcg gcggggccac gctggccatc ctctccgcct cggccgagga
     2521 cggcggcggg ctgggcgagt ggccgatcat cggccgggcc ggcgggcagg tggtcatggg
     2581 ttcgctggtg acgacgaagg gcggcgcggt ggcgctgctg agcgcctgat gacgcttaca
     2641 caaagaatac ctcacgcaaa ggcgcagaga agcaaaggac gcaaagaaga tagtcttgtt
     2701 cgcgtccttt gccccttttg cggctttgcg taaggttctt aagctaagga ggaggacatc
     2761 gtatggctcc atttgtccct gtgccccgcg cgttgcgccc ctgggcggtg gctggcgtgc
     2821 tgctggcggc cgtctt
//
LOCUS       DAOSMQ010000420         2835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35375_length_2835_cov_4.088129, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000420 DAOSMQ010000000
VERSION     DAOSMQ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35375_length_2835_cov_4.088129"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK829_12185"
     CDS             complement(<1..280)
                     /locus_tag="PK829_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42015.1"
                     /translation="MTGDSALIAAAEAQYDAYHLDDLPPALQRHSLDYYYLSVYPSLA
                     EMRPLAGGDAPPYPATIHNAYIHIPFCSGVCDFCSYYLVAISPRRRAAV"
     gene            389..1777
                     /locus_tag="PK829_12190"
     CDS             389..1777
                     /locus_tag="PK829_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU42016.1"
                     /translation="METGASVSSDKLDFKRVLPIFIIVLIDLLGLTIIIPLLPLYATA
                     FGANPFTIGLLGAAYPLMQFIGAPILGRLSDRYGRKPILIISQIGTLLGFILLGFANA
                     LPILFLSRIVDGLSGANIATAQAAISDVTTERNRTQGLGLIGAAFGLGFIIGPVIAFI
                     ALALSGNDYRVPAFAAAGFSLASILLTTFWFKETLPAAQRGASDRRAGVSFSAMGAAL
                     RHPTIGLLLFLIFGQQIVFGGFEQLLSIFTLSRLGLNASGNAVVFVFVGIILVAVQGG
                     LVGRWSRAYGDRRLIFAGLALLGVGLALIAFTPHQPPPWYSRAELEAELNSPETDLGS
                     EAPSATNSTVELPDDSNNGWFGLLWLLAAMIPVSIGGGMLQPSINSLITKRIGLAEIG
                     GMLGISAAFLSAANALAPVLGGAIFETVGPTALFLIWGVLMGALFLLAIRRLTPGREE
                     TAAPGLTRGGGH"
     gene            complement(1804..2238)
                     /locus_tag="PK829_12195"
     CDS             complement(1804..2238)
                     /locus_tag="PK829_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42017.1"
                     /translation="MDTSLTGAVPKRLAVWLAALVGAATCVLVPLLFAQQDSLWPLPG
                     LYFVQIALVGVLVLLFVAAGWGLKARRRWLPWAAAGILLAFVILGGFSIGPFLMPAFL
                     AFALVGFLVDWPDDGAMARHAGVLLVAAVAQAALMLLATLIA"
     gene            complement(2348..>2835)
                     /locus_tag="PK829_12200"
     CDS             complement(2348..>2835)
                     /locus_tag="PK829_12200"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOU42018.1"
                     /translation="ELARRYFTSHGPATLKDYTWWSGLDAAEARAGLAMVKGQLEHDV
                     AGREMYWFAATPVGELPSPSAHLLPFYDEYLVAYKDRRAVLPSEHNALADSRNIIFHQ
                     PILVDGRVAGTWLRELKADVVILRPTLFRHLTGAERDALGAAAERYGAFLGLRAELVM
                     A"
BASE COUNT          478 a          982 c          890 g          485 t
ORIGIN      
        1 ccacggccgc gcgccgccgg gggctgatgg cgacgagata gtagctgcaa aagtcgcaca
       61 cgccggagca gaagggaatg tgaatgtagg cgttgtggat ggtcgccggg taaggcggcg
      121 cgtcgccgcc ggccagcggg cgcatctccg ccagcgacgg gtagacgctg aggtaatagt
      181 agtcgagact atggcgttgc agcgcgggcg gcaggtcgtc gagatggtag gcgtcgtact
      241 gggcctcggc cgcggcgatg agggctgaat caccagtcat agggacggga gtgtaccaca
      301 gccacggccg cggtcacgac gtgcccgaca gagcaactgg ccgcctgcaa ccgttttctc
      361 tgcctacacg atgtggtacg ctcacggcat ggaaactgga gcctccgtgt cctctgacaa
      421 gcttgatttc aagcgcgtgc tgcccatctt catcattgtc ctgatcgatc tgcttggtct
      481 gacgattatc atcccgctgc tgccgcttta cgccaccgcc tttggggcca acccattcac
      541 cattgggttg ctgggcgcgg cctacccatt gatgcagttc atcggcgcgc ccatcctcgg
      601 ccgcctctcc gatcgctacg gccgcaaacc catcctgatc atcagccaga tcggcacgct
      661 actcggcttc atcctgctcg gtttcgccaa cgccctgccg atcctgttcc tgtcgcgcat
      721 cgtcgatggt ctatccggag ccaacatcgc cacggcgcag gcggccatca gcgacgtgac
      781 caccgagagg aaccgcaccc agggtctcgg cctcatcggc gcggccttcg gcctgggctt
      841 catcatcggg ccagtgatcg ccttcatcgc cctggccctt agcggcaatg actatcgcgt
      901 gccggccttc gccgccgcgg gcttctcgct ggcctccatc ctgctcacaa ccttctggtt
      961 caaggaaacg ctgcccgcag cccagcgcgg cgccagcgac cggcgcgccg gtgtcagctt
     1021 cagcgcgatg ggcgcggcct tgcgccaccc caccatcggc ctgttgctct tcctcatctt
     1081 cggccagcaa atcgtcttcg gcggcttcga acaactgctg tcgatcttca ccctcagccg
     1141 tctggggctg aacgcctccg gcaatgcggt cgtcttcgtt ttcgtcggca tcatcctcgt
     1201 cgccgtgcag ggcgggttgg tcggtcgttg gagccgcgcc tatggcgacc ggcggctgat
     1261 cttcgccggc ctggcgctcc tgggcgtggg gctggcgctc atcgccttca ccccgcacca
     1321 accgccgccg tggtattcgc gcgccgaact ggaagcggag ctgaattcgc cggaaacgga
     1381 cctcggttcc gaagcgccgt cagccaccaa ttcgaccgtg gagttgcccg atgacagcaa
     1441 caacggctgg ttcggcctgc tctggctcct ggccgcgatg atccccgtgt ccattggcgg
     1501 cggcatgttg cagccgagta tcaacagtct catcaccaag cggatcggcc tggcggagat
     1561 cgggggtatg ttgggcatct cggccgcctt cctcagcgcg gccaacgccc tggccccggt
     1621 tctgggcggc gcgatcttcg agacggttgg cccaacggcg ctattcctga tctggggggt
     1681 gctgatgggg gcgctgttcc tgcttgccat ccgtcgcctg acgccggggc gggaagagac
     1741 ggccgcgcca ggactgaccc gcggcggcgg gcactaggct ggcttgcgcg cgctgtcttt
     1801 gcctcaggcg atgagcgtcg ccagcagcat cagcgccgcc tgcgccaccg cggccaccaa
     1861 cagcacgccg gcgtgccgcg ccatcgcccc gtcgtccggc cagtccacca gaaagccaac
     1921 cagcgcgaag gcgagaaagg ccggcatcaa gaacggaccg atgctgaagc cgcccaggat
     1981 gacgaaggcc agcaggatgc ccgccgccgc ccagggcagc cagcgccggc gcgccttcag
     2041 cccccagccg gcggccacga acagcagcac cagcacgcct acgagggcga tctggacaaa
     2101 gtagaggccg ggcaagggcc agaggctatc ctgttgcgcg aagagaaggg gcaccaggac
     2161 acaggtggcc gcgccaacca gggctgccag ccagacggcc aatcgcttag gaaccgcacc
     2221 tgtcaatgaa gtatccatgg ctcactccgt cctgttgatt gcgccggcca tgcggcgctt
     2281 aggccttatc tcaagaacag gatagtagtg aggctacaat acgtcaagtg cgcggcctag
     2341 ccggtgttca ggccatcacc aactccgccc gcagccccaa aaacgcgcca tagcgctcgg
     2401 ccgccgcgcc cagcgcgtcc cgctccgccc cggtcagatg ccggaacagc gttggcctga
     2461 ggatcaccac gtccgccttc aattcgcgca accaggtgcc ggccactcgc ccatcgacga
     2521 ggattggctg atgaaaaatg atattgcggg agtcggccag ggcgttgtgc tccgagggca
     2581 ggacggcgcg gcgatctttg taggccacca gatactcatc gtagaacggc agcaggtggg
     2641 cgctcggcga cggaagctca cccacgggcg tcgccgcaaa ccagtacatc tcccggccag
     2701 ccacgtcgtg ctccaactgc cctttgacca tcgccaatcc cgcccgcgcc tcggccgcgt
     2761 ccagccccga ccaccaggtg tagtccttga gcgtcgccgg cccgtggctg gtgaagtagc
     2821 gccgcgccag ttcgg
//
LOCUS       DAOSMQ010000421         2833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35406_length_2833_cov_3.844132, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000421 DAOSMQ010000000
VERSION     DAOSMQ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35406_length_2833_cov_3.844132"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK829_12205"
     CDS             <1..708
                     /locus_tag="PK829_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOU42019.1"
                     /translation="TWGAAYAAAQVALSAAQALLGAPLVYALTRPPGHHAYSDLYGGY
                     CYLNNAAIAAHWLAQRGLRPAILDIDYHHGNGTQAIFYERDDVFFCSLHADPADEYPY
                     YSGYPDEMGAGQGEGATLNIPLPQATGEAGYLRALDWALAAVDAVSPDMLVVSLGFDT
                     LSGDPHGGMGLTPAAFRPIGQSLAALGRPTLLVQEGGYQLGMLGPALLALLEGLGEAT
                     GGGDAVAARLPKPDAAG"
     gene            complement(743..1138)
                     /gene="msrB"
                     /locus_tag="PK829_12210"
     CDS             complement(743..1138)
                     /gene="msrB"
                     /locus_tag="PK829_12210"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOU42020.1"
                     /translation="MTYKVQKTDTEWRQELTPEQYHIMREKGTERAFTGALYYNKEKG
                     IYSCAACGAPLFSSEQKFESGTGWPSFWAPANEQNVGQQTDRSLFMVRTEVHCANCGA
                     HLGHLFDDGPQPTGLRYCINSASLSFQKS"
     gene            complement(1239..2198)
                     /locus_tag="PK829_12215"
     CDS             complement(1239..2198)
                     /locus_tag="PK829_12215"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOU42021.1"
                     /translation="MSETPDWDSAWQGLEPQQPRSRRGCYGWALLAAVLLTIAVLALA
                     VFARQRGAGDDTGLLLPGTDAPATAAPGETPANPAAESTPAGPGLAATATLPGELLTP
                     SPPPVPDVIAGRFAPILDGDPTDWANIPTNTSPYTVFTGDGWDGSDDLGVTWQVGWDD
                     VYLYFAFSIADEVHSQSQTGNQTFRGDSIELQIDANRAGDFGPTLSADDFQISLSPGD
                     LGGLPPSAWLFQGTAGGDMIDAPAGQTITVAARPSADGYTLEAAVPWHDLGITPAPGL
                     VIGLAVNANDNDHPGTAAQELMKSNVPGRRFGDPTSWGTLTLQ"
     gene            2384..>2833
                     /locus_tag="PK829_12220"
     CDS             2384..>2833
                     /locus_tag="PK829_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOU42022.1"
                     /translation="MNPKIEQLRQLKKQAHLGGGEDRLAAQKAKGKLTARERIDLLLD
                     KGSFREVDALVVHRETNFGMDKKKYMGDSVVTGWGTIDGRLVYVFSQDFTVFGGSLSG
                     VHAEKICKIMDMAMRNGAPVIGINDSGGARIQEGVVSLAGYADVFLRN"
BASE COUNT          461 a          918 c          955 g          499 t
ORIGIN      
        1 acctggggcg ctgcctatgc cgcggcccag gtggctctct cggcggcgca agcgctcctg
       61 ggggcgccgc tcgtctacgc cctgacgcgc ccgcccggcc accacgccta ctccgatctc
      121 tacggcggct actgctacct gaataacgct gccattgccg cccactggct ggcccagcgc
      181 ggccttcgcc cggccattct cgacatcgac taccaccacg gcaacggtac gcaggccatc
      241 ttctatgagc gcgacgacgt cttcttctgc tcgctccacg ccgacccggc cgacgagtat
      301 ccctactaca gcggttatcc tgatgagatg ggcgcgggcc agggcgaggg tgcgacgctc
      361 aacatcccgc tgccgcaggc caccggcgaa gccggctacc tgcgagcgct cgattgggcg
      421 ctggcggccg tcgatgccgt ctcgcccgac atgttggtcg tctcgctggg cttcgacacc
      481 ctgtctggcg acccacacgg gggcatgggc ctgacgccgg cggcgttccg gcccattggg
      541 cagagtctgg cggcgctggg gcggcccacc ctgctggtgc aggagggcgg ctatcaactg
      601 gggatgctgg ggccggcgct gttggcgctg ttggaggggc taggcgaggc gacaggcggg
      661 ggcgacgcgg tcgccgcccg cctgccaaag ccggacgcgg ccggctagac cagcgtcggc
      721 cggctaaacc ggcgtcggcc ggctatgact tctggaagct tagcgaggct gagttgatgc
      781 aataacgcag gcctgtcggc tgcgggccgt cgtcgaagag gtggcccagg tgtgcgccgc
      841 agttggcgca atgcacctcg gtgcgcacca tgaacaggct gcggtcggtc tgctggccga
      901 cgttctgctc gttcgcgggt gcccagaagc tcggccagcc ggtgccagac tcgaacttct
      961 gctccgagct aaatagcggc gcgccgcagg cagcgcagct atagatgccc ttctccttgt
     1021 tgtaatacaa cgcgccggta aaggcccgct cggtgccctt ctcgcgcata atgtggtact
     1081 gctccggcgt caactcctgt cgccactccg tgtcggtctt ctgcactttg taggtcatgt
     1141 catcgctcct atcattctaa gctaccctcg atcttatgcc gggtgcgttg gagagtgatt
     1201 agaacgccgg cgggcgtaag cctcctgtaa gcttcgcgtc actgaagcgt cagcgtgccc
     1261 cacgatgtcg ggtcgccaaa gcggcggccc gggacgttgg acttcatcag ttcttgcgcc
     1321 gctgtgcccg ggtggtcgtt gtcgttggcg ttgacggcca ggccaatgac caggccgggc
     1381 gccggggtga tgcccaggtc gtgccagggg acggctgcct ccagggtgta gccatcggcg
     1441 ctggggcgcg cggctacggt gatggtctgc ccggccgggg cgtcgatcat gtcgccgccg
     1501 gccgtgccct ggaacagcca tgccgacggc ggtaggccac ccaggtcgcc gggcgagagg
     1561 ctgatctgga aatcgtcggc gctcagcgtg gggccgaaat cgccggcgcg attggcgtcg
     1621 atctgcaact cgatgctgtc gccgcgaaag gtctggttgc cggtctggct ctgcgagtgg
     1681 acttcgtcgg cgatgctgaa ggcgaagtag agatagacat cgtcccagcc cacctgccag
     1741 gtcacgccca ggtcgtcgct gccgtcccag ccgtcgccag taaagaccgt gtagggcgag
     1801 gtgttggtgg ggatgttggc ccagtcggtg gggtcgccat ccagaatggg cgcgaaacgg
     1861 ccggcgatga catcaggcac gggcggcggc gagggcgtca gcagctcgcc gggcagcgtt
     1921 gccgtggcag ccaggccagg gccggcgggt gtgctctcgg ccgcggggtt ggccggggtt
     1981 tcgccggggg cggccgtggc cggcgcgtcc gtgccgggca gcaacaggcc cgtgtcgtcg
     2041 ccggccccgc gctgccgggc aaagacggcc agggccaaca ccgcaatggt gagtaatacc
     2101 gctgccagta gtgcccagcc atagcaacca cgacgcgacc gcggctgttg cggctctaat
     2161 ccctgccagg cactatccca atctggtgtc tcgctcatga cgctaaacgg tagcgcgaat
     2221 cagtggccgc ggcaaggaac cggcccgcca gacagggttt ttgatctgag cgcgtcgatc
     2281 tgacagcccg tgcctcactt ggacgcctca tccccttgtc atataatgca ccgacaacca
     2341 cataaatcca ttgatcccag gaggtagctg accggcgcag cctatgaacc ctaagatcga
     2401 acaactccgc caactcaaaa agcaggctca tctcggcggc ggtgaggatc ggctggcggc
     2461 ccagaaggcc aaaggcaaat tgaccgcccg cgagcgcatc gatctgctgc tggacaaagg
     2521 ctctttccgc gaagtggatg ccctggtcgt ccaccgtgag accaacttcg gcatggacaa
     2581 gaagaaatac atgggcgata gcgtcgtcac tggctgggga accatcgacg gccgcctggt
     2641 ctacgtcttc agccaggact ttactgtctt cggcggcagc ctcagcggcg ttcatgccga
     2701 gaagatctgc aagatcatgg acatggccat gcgcaacggc gcgccggtca tcggcatcaa
     2761 cgactctggc ggggcgcgca tccaggaagg cgtcgtcagc ctggctggct acgccgatgt
     2821 cttcttgcgc aac
//
LOCUS       DAOSMQ010000422         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35452_length_2830_cov_4.495856, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000422 DAOSMQ010000000
VERSION     DAOSMQ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35452_length_2830_cov_4.495856"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK829_12225"
     CDS             complement(<1..353)
                     /locus_tag="PK829_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU42023.1"
                     /translation="MDYRKPAVFGENWSPSRYRVLPHFAAEQQVLARPDIQRILEIGC
                     GNGWNMSRFAQDGHVAFGLDVVPERVSLAAAHGPAMLGDGLRLPVGDGCLDMVYIQHV
                     LHHIGDVERALAEVRR"
     gene            complement(369..2579)
                     /locus_tag="PK829_12230"
     CDS             complement(369..2579)
                     /locus_tag="PK829_12230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42024.1"
                     /translation="MYRGAEQPAHPAPNSVADPLAVELTIIVPTRNEVANVAPLLDRL
                     GAALAGRSFEVLFVDDSTDATPQVITAEAAARPFPVRVIARRPEQRNGLSGAVVEGIA
                     SASGRWLCVIDADLQHPPELIPRLLAQASRTGADIVVASRQANLLGPVGLSRARAFTS
                     QTLTILARMVFPRVLKNVSDPLTGFFLARRAAVDPAVLQPEGFKILLELLARHPDLRV
                     TELHFDFAPRHDGQSKAGLNEGMRFFRHLTRLRLTVNQHLIRFLLLIAGIMAGNLALL
                     VALAGGAGWPVMRAAAVAGAATVAGMLVGEAWVFSDRPRGPARRRLAGVLLLGMFFLL
                     LLYLPGIWLLAVRLGLPYPLAALVAMLLAGFVYYAISEQWIWTRGLMMQPRASTYYNI
                     HGILTVASQVPLPDLATFQTDAEPARIDLQLRIDRHGTPSRVPNAICYDEHLGRFGFG
                     LTVIPGDFTEIVVSPLLETSPAFLYTNVVEPVLRWMLLARGQALPRLAGVVIEPEANG
                     PDSGRAILFAGQADMGYGLARLCEEGRAAFLGDDRLLLGRDRRARSFPKPVTARRDLT
                     PPTGALRAALALGLQRLLYTRPVRRLGLWLSNRHLPAATLNTYLQRLIPQPKYPLHEL
                     VPGLAYGQSSPLGPLLWVERDGATTTPLEQMLDEGDGALGFQPFPLLRSELAHWDGRD
                     WAAEERAILIAALAGCEPIAWRPAERWWEQIDRAMRAGTAGTALDPHTLAVEPA"
BASE COUNT          498 a          912 c         1020 g          400 t
ORIGIN      
        1 cgccgcactt cggccaatgc ccgctccaca tcgccaatgt ggtgcaggac gtgttgaatg
       61 tagaccatgt ccaggcagcc gtcgccgaca ggcagccgca ggccgtcgcc cagcatggcc
      121 gggccgtggg ccgcggccag ggagacgcgc tccggcacta cgtctagccc gaaggcgacg
      181 tggccatcct gcgcgaagcg gctcatgttc cagccattgc cgcagccgat ctccaggatg
      241 cgctggatgt cgggccgggc cagaacctgc tgctcggcgg caaagtgggg cagaacacgg
      301 tagcggctgg gggaccaatt ctcgccgaag acggctggtt tgcgatagtc catatccgaa
      361 aacactcctc aggctggctc caccgccagc gtgtgcgggt ccagtgccgt cccggctgtt
      421 ccggcgcgca tggcccggtc gatctgctcc caccagcgct cggccggccg ccaggcgatc
      481 ggctcacagc cggccagggc ggcgataagg atggcccgct cctcggcggc ccagtcgcgg
      541 ccgtcccagt gggccagttc gctgcgcagc agggggaaag gctggaagcc gagcgcgccg
      601 tcgccctcgt cgagcatctg ctccagaggc gtggtcgtcg cgccgtcgcg ctcgacccac
      661 agtagggggc cgagtgggct ggactggccg taggccagac cgggcacaag ctcgtggagg
      721 ggatacttcg gctgggggat aaggcgttgc agataagtat tgagcgtcgc cgcgggcagg
      781 tggcggttgc tgagccacag acccagccgg cgcacagggc gggtgtagag caggcgctgg
      841 aggcccaggg cgagggccgc ccgcagcgcg ccggtcggtg gcgtcaggtc gcggcgggcg
      901 gtgacgggct tggggaaact gcgggcgcgg cggtcgcggc cgaggaggag gcggtcgtcg
      961 ccgaggaagg cggcgcgccc ctcttcgcat agccgcgcaa ggccatagcc catgtctgcc
     1021 tgcccggcga aaaggatagc ccggccactg tctgggccgt ttgcctccgg ctcgatgaca
     1081 acgccggcga gccgcggcag ggcttggccg cgcgccagca gcatccagcg cagcaccggt
     1141 tcgacgacat tggtgtagag aaacgccggc gaagtctcta acaagggcga gacgacgatc
     1201 tcggtgaagt cgcccggaat gacagtcagg ccgaagccga agcggccgag atgctcgtcg
     1261 tagcagatgg cgttgggcac gcggctgggc gtgccgtgcc ggtcgatgcg tagttgcagg
     1321 tcgatgcgcg ccggctctgc gtccgtctgg aacgtggcca gatcggggag cggaacctgt
     1381 gaagccactg tcagaatgcc gtggatattg tagtaggtgc tggcccgtgg ttgcatcatt
     1441 agcccgcgtg tccagatcca ctgctcggag atggcatagt acacaaagcc ggccagcagc
     1501 atcgccacca gcgcggccag cgggtagggc aggcccaggc gcacggccag tagccagatg
     1561 ccgggcaagt agagcagcag caggaagaac atgcccagca gcagcacccc ggctagacgc
     1621 cgccgcgccg ggccgcgcgg ccggtcgctg aagacccacg cctcgcccac caacatgccg
     1681 gcaacggtgg cggccccggc cacagccgcg gcccgcatca ccggccagcc cgcgccgccc
     1741 gccagggcca ctaaaagcgc caggttgccg gccatgatgc cggctatcag caaaaggaag
     1801 cgaatgagat gctggttaac cgtcaggcgc agccgcgtca ggtggcggaa aaagcgcatg
     1861 ccctcattga gaccggcctt gctctggcca tcgtggcgcg gggcgaagtc gaaatgcagc
     1921 tcggtgacgc gcaggtcggg atggcgggcg agaagctcca gcaaaatctt gaagccctcc
     1981 ggctggagga cggccggatc gacggcggcg cggcgggcca aaaagaagcc cgtcagcggg
     2041 tcggagacgt ttttcaggac gcgcgggaag accatgcggg ccagaatggt cagcgtctgc
     2101 gacgtgaacg cccgcgcccg gctcagcccg actggcccca acaggttggc ctgccggctg
     2161 gcgacgacga tatcggcccc ggtgcggctg gcctgtgcca acaggcgggg aatgagttcg
     2221 ggcgggtgtt gcaagtcggc gtcgatgacg cacagccagc gcccgctggc cgaggcgatg
     2281 ccctccacaa ccgctccgct caggccattg cgctgctctg gccgacgagc gatgacccgc
     2341 accgggaacg gccgtgccgc ggcttcggca gtgatgacct ggggcgttgc gtcggtactg
     2401 tcgtcgacga atagaacctc gaaagagcgc ccggccaacg cggctcccag ccgatcgagc
     2461 agtggggcga cgttagctac ctcgttgcgc gttggcacga taatggtcag ctccacggcg
     2521 agcgggtcgg cgacggaatt aggcgcgggg tgggccggct gctctgctcc tcgatacatg
     2581 gtcgtttgct gctggggcgg ccggcgcgcc ggccgcagga tagggcgata atagcacagc
     2641 ggcgcggccg atgcgactag gcgaacgggc cgggaatggg tatgattcta ggacggccgc
     2701 cacaggcagc cacaacgcga ctatggccca gaacgatttc agcatttcgg ccttcttccc
     2761 ggcttacaac gacgccggca cgatcaccag catggtcatc accttgctcc tgacgctgcg
     2821 cgaactgacc
//
LOCUS       DAOSMQ010000423         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35577_length_2822_cov_5.108421, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000423 DAOSMQ010000000
VERSION     DAOSMQ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35577_length_2822_cov_5.108421"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PK829_12235"
     CDS             <1..449
                     /locus_tag="PK829_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42025.1"
                     /translation="AGVGQDAVQRRAELVGHVGEELALEPVGPLGAFLGLDEVGRLGR
                     VLGPRGLMPNPRAGTVAPAADLPRLIDEAKAGRVEFRVDKTSNIHAPIGKASFSQEQL
                     NENLTAFIGAVKRARPAAAKGTYIQKITVANTMGPGIRLDPVEAAL"
     gene            644..1213
                     /gene="rplJ"
                     /locus_tag="PK829_12240"
     CDS             644..1213
                     /gene="rplJ"
                     /locus_tag="PK829_12240"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOU42026.1"
                     /translation="MAIDKERKDELVNEYRDLLQRSSAIFLTRYGGMTVKEMEALRLK
                     IGDANGQINVTKNTLLGIALEQTDHVPPSELLNGQLATSFALGEASALAKVLVDQAKA
                     NDKLKIVGGMLGNRVLTPKEVESLATLPSLDQLRAQILGLISAPAQGIVSAVANGVRQ
                     VVNVLDAYAKKDDSGAAPDAPATMDAAAA"
     gene            1298..1690
                     /gene="rplL"
                     /locus_tag="PK829_12245"
     CDS             1298..1690
                     /gene="rplL"
                     /locus_tag="PK829_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004669074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOU42027.1"
                     /translation="MADLDKIVQELSSLTLLEAAELTKKLEEVWGVSAAAPMAMGMMP
                     GMMAGGGAAAPAEEVEEQTEFSVKLKDIGPKKIEVIKAVRTLTNLGLKEAKDAVEGLS
                     VIMEAVTKEQAAEAKKTLEEAGAVVEVK"
     gene            1764..2435
                     /locus_tag="PK829_12250"
     CDS             1764..2435
                     /locus_tag="PK829_12250"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOU42028.1"
                     /translation="MDDYHFVLASQSPRRRQLLRLLGYPFEVYVADVDEDVHLSAGPA
                     DYVQLTAAQKAEAAARALGTPTTPTLIIAADTTVALDGAILGKPADAGHARHMLTALR
                     GQTHAVYTGLCVVDTASGREVRALHTTAVTMRAYSPAEIDAYVATGDPLDKAGAYAIQ
                     YAAFAPVAALDGCYLNVVGLSLCGLIAALAEFGVPCRARRAELRAAHDGYLCPLLEQV
                     WGQCQ"
     gene            2447..>2822
                     /locus_tag="PK829_12255"
     CDS             2447..>2822
                     /locus_tag="PK829_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42029.1"
                     /translation="MERMARLRPALGRIAFALLSAAILLVFSEKLFWYVTGYGILDLL
                     VGYFFPATILLAVVAAFRVRRPAAVFLAGAVFGFVAEGMLTSTLYEGGPLAWFSASYT
                     PLAWHAPLSVFFGWWWLRRELIA"
BASE COUNT          512 a          904 c          939 g          467 t
ORIGIN      
        1 aggctggcgt aggacaggat gccgtgcagc ggcgtgcgga actcgtggga catgttggtg
       61 aggaactcgc tcttgagccg gttggcccgc tcggcgcttt ccttggcctg gacgaggtcg
      121 gccgtctggg ccgcgtgctc ggcccgcgcg gcctgatgcc taacccgcgc gcgggcacag
      181 tggccccggc cgcggacctg ccccgcctga tcgacgaggc caaggccggc cgcgtggagt
      241 tccgcgtcga caagacctcc aacatccacg cgcccatcgg caaggccagc ttcagccagg
      301 agcagttgaa cgagaacctg accgccttca tcggcgcggt caaacgcgcc cgcccggccg
      361 cggccaaggg cacctacatc cagaagatca cggtcgctaa cacgatgggg cctggcattc
      421 gccttgaccc ggtggaggcc gcgctctaga cgcacatcaa caaccgtagt ctctaccgga
      481 gacagccggc ggggcgccat ttggcaagcc ccttaatatt gccggccgag gtaggacact
      541 acagcccacg ccgggtggcc aacgccatcc gcctgacctg taaagtcctc ctatcggccg
      601 cgccggtacg gaggattttt tcttgcagag gaggtgaaaa cctttggcaa tcgacaagga
      661 acgcaaggat gagctggtaa acgagtatcg cgacctgctc cagcgcagtt cggccatctt
      721 tctgacccgc tacggcggca tgactgtcaa ggagatggag gcgctacggc tgaagatcgg
      781 cgacgccaac gggcagatca acgtgaccaa gaacacgctg ctcggcattg ccttagagca
      841 gaccgaccac gtgccgccca gcgaactgct gaacggccag ttggcgacga gcttcgccct
      901 gggcgaggcc tcagcgctgg cgaaggtact ggtggatcag gccaaggcca atgacaagct
      961 gaagatcgtc ggcggcatgc tcggcaatcg cgtgctgacc ccgaaagagg ttgagtcgct
     1021 ggccacactg ccctcgctcg accagctccg cgcccagatt ctgggcctga tcagtgcgcc
     1081 cgcgcagggc attgtctcag ccgtcgccaa tggtgtgcgc caggtcgtca acgtcctgga
     1141 cgcctacgcc aagaaggacg acagcggcgc agcgccggac gcaccggcga caatggacgc
     1201 cgccgcggcc taagaggctt gagactatct tacccattga caacacaagg cggacacaac
     1261 gtccgcgcga gaagcaatct aggagtgata taagacaatg gcagatttgg acaagatcgt
     1321 acaggaactg agcagcctga ccctgttgga agcggccgag ctgaccaaga agctggaaga
     1381 ggtatggggc gtcagcgccg ccgcgccgat ggcgatgggc atgatgccgg gcatgatggc
     1441 cggcggtggc gccgcagcgc cggccgagga agtcgaggag cagacggagt tctccgtcaa
     1501 gctgaaggac atcggcccga agaagatcga ggtcatcaag gccgtgcgca cgctgacgaa
     1561 cctgggcctg aaagaggcca aagacgccgt tgaaggcttg agcgtcatca tggaagccgt
     1621 gactaaggaa caggcggccg aggccaagaa gacccttgaa gaggccggcg cggttgtcga
     1681 ggtcaagtag ttccgactct agtggaatct ctgacgagga ggccggtata cttctgggta
     1741 gaccggtctc tttttcattg gtgatggacg actaccactt tgttctggct tcgcagtcgc
     1801 cgcggcggcg gcaattgctg aggctgctag gctacccgtt cgaggtctac gtcgctgacg
     1861 tggacgagga cgttcacctg agcgccggcc cggccgacta tgtgcagcta acggcggcgc
     1921 agaaggcaga ggccgcggcc cgcgcgctgg gtacgccgac cacgcccaca ctgatcattg
     1981 ccgccgacac gaccgtagcc cttgacgggg ccatcctggg caagccggcc gacgccggcc
     2041 acgcccgaca catgctgacg gcgctgcgcg gccagacaca cgcggtctac accggccttt
     2101 gcgtcgtcga tacggccagt gggcgcgagg tgcgggcgct gcacaccacg gcggtgacca
     2161 tgcgcgctta cagtccggcg gagatcgacg cctacgtggc caccggcgac ccactggaca
     2221 aggccggcgc ctatgccatc caatacgctg ctttcgctcc cgtggccgcg ctggacggct
     2281 gctatctaaa cgtcgtcggg ctgtcgctct gcgggctaat cgcggcgctg gcggagttcg
     2341 gcgtgccctg ccgcgccagg cgggcggagc tgcgagccgc ccacgacggc tacctttgcc
     2401 ccttgctgga gcaggtctgg gggcaatgcc agtaaagagc cgcgctgtgg aacggatggc
     2461 gcgcctgcgc ccggcactgg ggcgtattgc cttcgcgctg ctgtcggccg ccattctgct
     2521 cgttttctcc gagaagctct tctggtacgt caccggctat ggcatcctcg acctactggt
     2581 gggctacttc ttccccgcca ccatcctgct ggccgtcgtc gctgccttcc gcgtgcgccg
     2641 gccggcggcc gtcttcctgg ccggagcggt tttcggcttc gtcgccgagg ggatgctgac
     2701 cagcacgctc tacgagggtg ggccgctggc ctggttcagc gccagctaca cgccgctggc
     2761 ctggcacgcg ccgctgagcg tgttcttcgg ctggtggtgg ctgcggcgcg aactgattgc
     2821 cg
//
LOCUS       DAOSMQ010000424         2808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35815_length_2808_cov_3.836179, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000424 DAOSMQ010000000
VERSION     DAOSMQ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35815_length_2808_cov_3.836179"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /locus_tag="PK829_12260"
     CDS             <1..2028
                     /locus_tag="PK829_12260"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42030.1"
                     /translation="ATTTATRTPTATTTRTPTVTATRTPTATGTLTATPTRTPTTTAT
                     RTPTATATRTPTRTPTATATATRQTTGYTLLLPVISNGTVFEPVQFAGQFGGGLQAVA
                     VRGNLAYAGLGARLAVLDVANPAAPALLGQTTLLSSVIEDVAVGGDYAYAAVGDDGLR
                     VVNISNPAEPVIVTTMDTPGSAQKMTLAGNILYVADLDGGLRLINVVNPAAPVEIGYF
                     DNNWLVYDVAVAGNYAFITNGGAGLRIINVANPAAPVEVGSLDATLPYLDHVAVSGPY
                     AYLTEADYYGGGGGLLRVVNIANPAAPFETGSTEIGWSNSVAVAGQTVYVAGYSGLQL
                     VNVANPAAPVVSDDYWTSNGEFADVAVAGGYAYIAAAQSLTILNTADPSQIQWVGTFQ
                     TPGDSHDIAVVGNYAYSIAYSSGLRIINVSNPAVPADTSFYGSIGRTSGVSLSGQYAY
                     LASDVGLEIVNVTNPAGPTLAGGLDQGLVMNDVFVVGRYAYVAAGSDGLRVIDVSNPA
                     APAQVGFHNIEYAITVAVEGAYAYVGGAQGLYIVNIANPAAPAQVSYFDTGDNVRGVA
                     AAGRYVYVTDRNEVRILDVSNPAAPVQVGVVDEGIEEANWWANDVAVAGRYLYVAWGD
                     GLRVLDVANPAAPVSVGFYDTPGTANGVAVAGDYVYVADGAGGVFVLRHNK"
     gene            complement(2167..>2808)
                     /locus_tag="PK829_12265"
     CDS             complement(2167..>2808)
                     /locus_tag="PK829_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOU42031.1"
                     /translation="GSKRLVRQAIQYAIDHQRPSVTLVHKGNIMKFTEGAFKNWGYEV
                     ATQEFGDDTVTEDDLWNKYDGRVPAGKILINDRIADNMLQQMITRTDEYSVLATLNLN
                     GDYISDAAAAQVGGLGMAPGGNIGDGLALFEATHGTAPKYAGKDMVNPGSLILSGAMM
                     LEYMGWNEAADLVVEAMGKTIQDKVVTYDLHRQIDGATKVSSSGFARAIVERM"
BASE COUNT          483 a          944 c          875 g          506 t
ORIGIN      
        1 gcaaccacta ccgctacccg caccccaacc gcgacaacga cccgcacccc gacggtgacg
       61 gccacgcgca cgccaaccgc taccggcact ctgacggcga cgccgacgcg cacgccaacc
      121 accacggcca cccgtacccc gaccgccacc gccacgcgga caccaacgcg cacgccaacc
      181 gccaccgcca ccgccacgcg ccagacgacc ggctatacgc tcctgttgcc tgtcatcagc
      241 aacgggacgg tgttcgagcc ggtgcagttc gccggccagt ttggcggcgg gttacaggcc
      301 gtggccgtgc gcggcaacct ggcctacgcc ggcctgggcg cgcggctggc cgtgctcgac
      361 gtggccaacc cggccgcgcc ggcgctgctg ggccagacga cgctgctatc gtcggtcatt
      421 gaggacgtgg ccgtgggcgg agattatgcc tatgcagcgg tcggcgacga cggcctgcgc
      481 gttgtcaata tcagcaaccc ggccgagccg gtgattgtca cgaccatgga cacgccgggc
      541 agcgctcaga aaatgaccct tgccggcaac atcctctacg tggccgacct tgacggtggt
      601 ctgcgcctca tcaacgtggt taacccggcc gcgccggtcg aaattggcta tttcgacaac
      661 aactggctcg tctacgacgt agcggtagcc ggcaactacg ccttcatcac caacggcggg
      721 gctggactgc gcattatcaa cgtggccaac ccggccgcgc cggtcgaggt cggctctctg
      781 gacgcgacgc tcccatatct agatcacgta gctgttagcg ggccgtacgc ttacctgaca
      841 gaagcagatt actacggtgg cggaggcggc ctcttgcgcg tggtcaacat cgccaacccg
      901 gccgcgccgt ttgagacggg ttccaccgag atcggctgga gcaactcggt cgccgtggcc
      961 ggccagaccg tttacgtagc tggctattcg ggcctgcaat tggtgaacgt tgccaatccg
     1021 gcggcgcccg tcgtcagcga cgactattgg accagcaatg gtgagtttgc cgacgtggcc
     1081 gtggcgggtg gctatgccta catcgccgca gcacagtcgc taacgatcct caacaccgcg
     1141 gatccttccc aaattcaatg ggtcggaacc ttccagacgc cgggagattc ccacgatata
     1201 gccgtagtcg gcaattacgc ctactccatc gcttactcgt ccggcctgcg gatcatcaac
     1261 gtatccaacc cggccgtccc ggccgacacc agtttctatg gtagcatcgg ccgcacctcc
     1321 ggcgtttcgc tcagcggaca atacgcttac ctggccagcg atgtgggcct ggagatagtg
     1381 aacgtaacca acccggctgg gccgacactg gcgggcgggc ttgaccaggg actcgtcatg
     1441 aacgacgtgt ttgttgttgg gcgctatgcc tacgtcgccg ctgggtcaga cggcctgcgt
     1501 gtgatcgacg tcagcaaccc ggccgcgccg gcgcaggtcg gcttccacaa catcgaatac
     1561 gccatcaccg tagccgtcga gggagcctat gcttatgttg gcggcgcgca ggggctttac
     1621 atcgtcaaca ttgccaaccc ggccgcgccg gcgcaggtca gctacttcga tacgggcgac
     1681 aacgtgcgcg gcgtggccgc ggccgggcgc tatgtctatg tcaccgaccg caacgaagta
     1741 cgcatcctcg acgtgagcaa cccggccgcg ccggtgcagg taggggttgt ggacgagggc
     1801 atcgaggagg ccaactggtg ggccaacgac gtagctgtgg ccgggcgcta cctctacgtc
     1861 gcctggggcg acgggctgcg ggtgctcgac gtggccaacc cggccgcgcc ggtgtcggtg
     1921 ggattctacg acacgccggg gacggccaat ggcgtggccg tggccggaga ttatgtctac
     1981 gtggctgatg gggcgggtgg ggtgtttgtt ttgcggcata acaagtagaa agtcgacatc
     2041 gacatcagtg gcaagacctc tgaggtttgc ggcaaacctc agaggtcttc tctaagccag
     2101 cgacgcggga tgccatcccg cgctacaact tatatccgcg ggataccatc ccgcgctaca
     2161 accaacttac atcctctcca caatcgcccg cgcaaacccc gacgagctga ccttcgtcgc
     2221 cccgtcgatc tgccggtgca ggtcgtaggt cacgaccttg tcctgaatgg tcttgcccat
     2281 cgcctcgacg accagatcgg cggcctcgtt ccagcccatg tactccagca tcatcgcccc
     2341 gctcaggatg aggctaccgg ggttgaccat gtccttgccg gcgtatttgg gggccgtgcc
     2401 gtgggtggcc tcgaacaggg ccaggccgtc gccgatgttg ccgccggggg ccatgcccag
     2461 cccgcccacc tgcgccgcag cggcgtcgct gatgtagtca ccgttcaggt tcagcgtggc
     2521 caggacgcta tactcgtccg tgcgggtgat catctgctgg agcatgttgt cggcgatgcg
     2581 gtcgttgatg aggatcttgc cggccgggac gcggccgtcg tacttgttcc acaggtcgtc
     2641 ctcggtcacg gtgtcgtcgc caaactcctg cgtcgccacc tcatagcccc agttcttgaa
     2701 ggccccctcg gtgaacttca tgatgttgcc cttgtgcacc agcgtcaccg acggccgctg
     2761 gtggtcgatg gcgtactgga tggcctggcg cactagccgc ttgctgcc
//
LOCUS       DAOSMQ010000425         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35831_length_2807_cov_5.173328, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000425 DAOSMQ010000000
VERSION     DAOSMQ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35831_length_2807_cov_5.173328"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /gene="argC"
                     /locus_tag="PK829_12270"
     CDS             <1..872
                     /gene="argC"
                     /locus_tag="PK829_12270"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOU42032.1"
                     /translation="PGAPALPLLAGADAPLEAADVVFLCLPHAAAAETALVALAAGKR
                     VIDLSADFRLRDAEVYRAWYAHNHPAPELLAEAVYGLTEVNRAALPGARLVANPGCYP
                     TSVLLALRPLLLSGIPLAGPIIADSASGVSGAGRTPTQTSHFVEVADNYSAYKIGRAH
                     RHLPEMEQAIGQWRAGAPPLIFSPHLLPVPRGILSTIYAQLDADASEDDLRARYRAAY
                     DGEPFVEVLPPGETASLAHVNHTNRCAIGLKLVGRTLILTSAIDNLVKGAAGQAVQNM
                     NAMFGCAETTGLL"
     gene            869..1666
                     /gene="argB"
                     /locus_tag="PK829_12275"
     CDS             869..1666
                     /gene="argB"
                     /locus_tag="PK829_12275"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOU42033.1"
                     /translation="MTGDNGHAPGGVHVIKIGGNEMNASGFLEALAAGLAQLAADAPL
                     VVVHGGGQDIATLQSRLGIEPVKVDGLRVTDAGSLAVAQMVLSGHTNKQVVRALLGAG
                     LDAVGLSGVDGRLLTCRRKRHPTVDLGLVGEVTAVRGDLLRGLLDGGITPVVSPISLG
                     EDGQTYNVNADEAAGAIAAALGAAQLDFVSNVPGVLAGGERLSQLTPAAAEALIANGV
                     ISGGMIPKVRTALDALARGVPRVRIVDLAGLAAAAGTVFHPGLTADR"
     gene            1765..>2807
                     /locus_tag="PK829_12280"
     CDS             1765..>2807
                     /locus_tag="PK829_12280"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HOU42034.1"
                     /translation="MDTQSIITDESQYLMQTYRRADIVLARGDGAYLFDTDGRRYLDF
                     MSGIAVAALGHADPDVTAAVAQQAATLTHVSNLFYTIPQVGLARRLVTHSFADRVFFT
                     NSGAEAIEGALKFARKWTRGRGQGKHEIVAFSGSFHGRTMGALSVTYKAAYREPFAPL
                     VPGVCFAPFNDLAAAAALISDATCAVVVEPVQGEGGIHAATPAFLRGLRALCNQHGAL
                     LIFDEIQCGLGRTGRLWAHEAAGVTPDIMTLAKPLANGLPIGAILLTEDVAAAVGPGE
                     HGSTFAGGPVVCRAGEVVFDRVSDPAFLAAVAANGQYLREQLRALASPLVVEVRGAGL
                     LVGVELKTAVAPIL"
BASE COUNT          395 a         1045 c          964 g          403 t
ORIGIN      
        1 tccccggtgc gccggccctg ccgctgctgg ccggcgcgga cgcgccactg gaggcggccg
       61 acgtcgtctt cctctgcctg ccccacgccg ccgcggcgga gacggccctc gtcgccctgg
      121 ccgcgggcaa gcgcgtcatc gacctgagcg ctgacttccg cctgcgtgac gccgaggtct
      181 atcgcgcctg gtatgcccac aaccaccccg cgccggaact gctggccgag gccgtctatg
      241 gcctgactga ggtcaaccgg gcggcgctgc cgggggcgcg gctggtggcc aaccccggct
      301 gctacccgac gagcgtcctg ctggcgctgc ggccgctgct gctgagcggc atccctctgg
      361 ccgggccgat catcgccgac agcgcctccg gcgtcagcgg cgcgggccgc acgccgacgc
      421 agacgagcca cttcgtcgag gtggccgaca actacagcgc ctacaagatc ggccgcgccc
      481 atcgccacct gccggagatg gagcaggcca tcggccagtg gcgggccggc gcgccgccgc
      541 tgatcttctc cccccacctg ctacccgtgc cgcgcggcat cctctccacc atctacgccc
      601 agctggacgc ggacgcgagc gaggacgacc tgcgcgcgcg ctaccgggcc gcctacgacg
      661 gcgagccgtt cgtcgaggtg ctgccgccgg gcgagacggc ctcgctggcc cacgtcaacc
      721 acaccaatcg ctgcgccatc ggcctgaagc tggtcggccg gacgctcatc ctgacctcgg
      781 ccatcgacaa tctggtcaaa ggggccgccg ggcaggccgt tcagaacatg aacgcgatgt
      841 tcggctgcgc cgagacgacg gggctgctat gaccggcgac aacgggcacg cgccgggcgg
      901 cgtccacgtc atcaagatcg gcggcaacga gatgaatgcc agcggttttc tggaggcgct
      961 ggccgcgggg ctggcgcaat tggccgcgga cgcgccgctg gtcgtcgtcc acggcggcgg
     1021 gcaggacatc gccacgctac agagccggct gggcatcgag ccggttaagg tcgatggtct
     1081 gcgcgtgacc gacgccgggt cgctggccgt ggcccagatg gtgctctccg gtcacaccaa
     1141 caagcaggtc gtccgggcgc tgttgggcgc ggggctggat gccgtcgggt tgagcggcgt
     1201 tgacggccgg ctgctgacct gtcgccgcaa gcgccacccg acggttgacc tggggctggt
     1261 gggcgaggtg accgccgtgc gcggcgatct gttgcgcggg ctgctggatg ggggcatcac
     1321 cccggtcgtc tcgcctatct ccctgggcga ggacggccag acctacaacg tcaacgccga
     1381 cgaggccgcc ggggccatcg ccgccgcgct gggggcggcg caactcgatt tcgtttccaa
     1441 tgtgcccggc gtgctggccg gcggcgagcg gctgtcgcaa ctgacgccag ccgcggccga
     1501 ggcgctcatc gccaacggcg tcatcagcgg cggcatgatc cccaaggtgc gcaccgccct
     1561 cgatgccctg gcccgcggcg tcccccgcgt gcgcatcgtc gatctcgccg ggctggccgc
     1621 tgcggccggg acagtcttcc acccagggct gaccgccgac cgctgacggc cgaccgctga
     1681 ccacggacga cagacgacag accacagacc tactgaccca ctgacctact gacccactgg
     1741 cttaccgacc actgaccacc aactatggac acccaatcga tcatcaccga cgaatcccaa
     1801 tatctcatgc agacctaccg ccgggccgac atcgtgctgg cccggggcga cggcgcttac
     1861 ctgttcgaca ccgacggccg ccgctacctc gacttcatgt ccggcatcgc cgtggccgcg
     1921 ctggggcacg ccgacccgga cgtgaccgcc gccgtggccc aacaggcggc gacgctgacg
     1981 cacgtcagca acctcttcta caccatcccc caggtcgggc tggcccggcg gctggtgacc
     2041 cactccttcg ccgaccgcgt cttcttcacc aacagtggcg cggaggccat cgaaggcgcg
     2101 ctgaagttcg cccgcaagtg gacgcgcggc cgcggccagg gcaagcacga gatcgtcgcc
     2161 ttcagcggca gcttccacgg ccggacgatg ggcgcgctgt cggtcaccta caaagccgcc
     2221 taccgcgagc cgttcgcgcc gctggtgccg ggcgtctgct tcgcgccgtt caacgacctc
     2281 gccgcggccg ccgcgctcat ctccgacgct acctgcgccg tggtcgtcga gccggtgcag
     2341 ggcgagggcg gcatccacgc cgcgacgccg gccttcctgc gcggcctgcg ggcgctgtgc
     2401 aaccaacacg gagcgctgct catcttcgat gaaatccagt gtggcctggg gcggacgggg
     2461 cggctgtggg cgcacgaagc cgccggggtc acgcccgaca tcatgaccct ggccaagccg
     2521 ctggccaacg gcctgcccat tggcgcgatt ttgctgaccg aagacgtggc cgcggccgtc
     2581 ggcccgggcg agcacggcag cacttttgct ggcgggccgg tcgtctgccg ggcgggcgag
     2641 gtcgtcttcg accgcgttag cgacccggct ttcctggccg ccgtggccgc caatggccaa
     2701 tacctgcgcg agcagttgcg cgccctggcc agcccgctgg tcgtcgaggt gcgcggcgcg
     2761 gggctgctgg tcggcgtgga actgaagacg gccgtcgcgc ccatcct
//
LOCUS       DAOSMQ010000426         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35834_length_2807_cov_4.494549, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000426 DAOSMQ010000000
VERSION     DAOSMQ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35834_length_2807_cov_4.494549"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..2170
                     /locus_tag="PK829_12285"
     CDS             137..2170
                     /locus_tag="PK829_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOU42035.1"
                     /translation="MTTRTAFPRPVREIENLWIPLPDGARLAARVWLPADATGAPVPA
                     LLEFLPYRKSDGTIVRDALRQPYYAGHGYAVLRVDMRGSGDSDGVLLDEYTTQEHDDA
                     VAVIAWIAAQPWCDGNVGMMGISWGGFNSLQVAARRPPALRAVVVIGFTDDRYADDVH
                     YMGGCVITSQMLSWSAVMHAYNALPPDPRWVGERWRAMWLERLAGSPPFIETWLAHQT
                     RDAYWRHGSVGEDYRAIAVPVYAVGGWADSYNNSVPRLLAGLSGPRKGLIGPWAHTFP
                     EMGGPGPAIGFLQDSLRWWDYWLKGIDTGIMAEPMLRCWLQDSVPPATYYAERPGRWV
                     AEPSWPSPNVSARALYLTGDGAAGRLSARPEAEAALRIRGRLGYGLDVGQWGAYGTPG
                     EWPGDQRAADGECLTFDSPPAGAPEDILGYPEVELLLSADQPLALVAVRLCDVAPDGA
                     STLVSWGLLNLTHRDGHEFPEPLEPGRCYRVTVPLNVSGHCLAAGHRWRVAISPVYTR
                     HAWPSPEAVTLTLFAGAGCCLRLPVRVPSPADAALADFPPPEMAAPAAREALRPGRSR
                     KTISHDLIDGWTELRIEDDGGRTRYSGSGMEVEDGVTEITRVREGDPLSLRVDIVRHL
                     ELQRGDWRVRVETRCAMSADATHFHVSHHLDAYEGDTRVFARADVKSIPRHLV"
     gene            2180..>2807
                     /locus_tag="PK829_12290"
     CDS             2180..>2807
                     /locus_tag="PK829_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOU42036.1"
                     /translation="MTKPRLRDLGITIGEFPPGPHNAITDVAGTLVGHTTLVYDEPRV
                     ARTGVTIIMPRGHDSWRQPSFGASHSFNGCGEMTGLLWLDESGQLSHPIALTSTHHVG
                     TVHEALIAYAQERGHTEQSSLPVVGETWDGWLNDMDGFHVKAEHVTSALANVSAGPVA
                     EGNVGGGTGMICHEFKGGIGTASRVVATQSGTWTIGALVQANHGAREDF"
BASE COUNT          406 a         1021 c          945 g          435 t
ORIGIN      
        1 aacgacgtgt tcacaacgcg ttgacgatta taccatctgc ggctatgatg gcatgtaggc
       61 tagagcgctt gtcagagaga ggtatcttgc ctgtccggct gtctctcctt catccttcat
      121 ccctcatccc tccgccatga ccacccgcac cgccttcccc cgccccgtcc gcgaaatcga
      181 aaacctgtgg attcccctgc ccgacggcgc gcgcctggcc gcccgcgtct ggctgccggc
      241 cgacgccacc ggcgcgcccg tgccggccct gctggagttc ctgccctatc gcaagagcga
      301 cggcacgatt gtgcgcgacg ccctgcgcca gccctactac gccggccacg gctacgctgt
      361 cttgcgcgtc gatatgcgtg gcagcggcga ctccgacggc gtgctccttg acgagtacac
      421 gacgcaggaa cacgacgacg ccgtcgccgt catcgcctgg atcgccgcgc agccgtggtg
      481 cgacggcaat gtgggcatga tgggcatctc ctggggcggg ttcaacagcc tgcaggtggc
      541 cgcgcggcgg ccgccggcat tgcgggccgt agtcgtcatc ggcttcaccg acgaccgcta
      601 cgccgacgat gttcactaca tgggcggctg cgtcatcacc tcgcagatgc tgtcctggtc
      661 ggcggtcatg cacgcctaca atgccctgcc gcccgatccg cgctgggtgg gcgagcgctg
      721 gcgggcgatg tggctggagc gactggccgg ctcgccgccg ttcatcgaga cgtggctggc
      781 ccaccagacg cgcgacgcct actggcggca tggctccgtg ggcgaggact accgcgccat
      841 cgccgtgccg gtctacgccg tcggcggctg ggccgacagc tacaataact ccgtgccgcg
      901 cctgctggcc gggctgagcg ggccgcgcaa ggggctgatc ggcccgtggg cgcatacgtt
      961 cccggagatg ggcggccccg gcccggccat cggcttcctg caggacagcc tgcggtggtg
     1021 ggactactgg ctgaagggca tcgacacggg catcatggcc gagccgatgt tgcgctgctg
     1081 gctgcaggac agcgtgcccc cggcaaccta ctacgccgag cggcccggcc gctgggtggc
     1141 cgagccgtca tggccgtcgc ccaacgtcag tgcgcgggcg ctctacctga ccggcgacgg
     1201 cgcggccggc cgtttgagcg cccggccgga ggccgaggcc gcgctgcgta tccgcggacg
     1261 cctcggctac ggcctggacg tcggccagtg gggcgcgtat ggcacgccgg gcgagtggcc
     1321 gggcgaccag cgcgccgccg acggcgagtg cctgaccttc gacagcccgc cggctggcgc
     1381 gccggaggac atcctcggct acccggaggt ggaactgctg ctgagcgccg accagccgct
     1441 ggcgttggtg gccgtgcgcc tgtgcgacgt ggctcccgac ggcgcgtcaa cgctggtcag
     1501 ttgggggctg ctcaacctca cccaccgtga cggccacgag ttcccggagc cgttggagcc
     1561 gggacggtgc taccgcgtca cagttccgtt gaacgtcagc ggccactgcc tggccgcggg
     1621 ccatcgctgg cgcgtggcca tttcgccggt ctatacgcgc cacgcctggc cgtcgccaga
     1681 ggcagtgacc ctcactctct ttgctggggc cggctgctgt ctgcgcctgc ctgtgcgcgt
     1741 cccctcgccg gccgacgcgg ccctggccga cttcccgccg ccggagatgg ccgcgcccgc
     1801 cgcccgcgag gcgttgcgcc ccggccgcag ccgcaaaacc atcagccacg acctcattga
     1861 cggctggacg gaactacgca tcgaggacga cggcggccgg acgcgctata gcggctccgg
     1921 catggaggtc gaggacggcg tgactgagat cacccgcgtc cgcgagggcg acccgctgtc
     1981 gttgcgcgtc gatatcgtcc gccacctcga attgcagcgc ggcgactggc gcgtgcgcgt
     2041 ggaaacgcgc tgcgccatga gcgccgacgc cacccacttc cacgtcagcc atcatctgga
     2101 cgcctatgaa ggcgatacgc gcgtctttgc tcgcgcggac gtcaagtcca ttccccgcca
     2161 tttggtttga aggtaagcca tgacgaaacc cagactacgt gatctcggca tcaccatcgg
     2221 cgaattcccg cccggccccc acaacgccat caccgacgtg gccggcacgc ttgttggcca
     2281 tacgacgctg gtctacgacg agccgcgcgt ggcccgcacc ggcgtcacca tcatcatgcc
     2341 ccgcggccat gacagctggc ggcagccgtc gttcggcgcg tcccactcct tcaacggctg
     2401 cggcgagatg accggcctgc tctggctgga cgagtcgggc cagctcagcc accccatcgc
     2461 cctgaccagc acccaccacg tcggcaccgt ccacgaggcg ctcatcgcct acgcccagga
     2521 gcgcggccac accgagcagt cgagcctgcc cgttgtcggc gagacgtggg acggctggct
     2581 caacgacatg gacggctttc atgtcaaggc cgagcacgtc accagcgccc tggccaacgt
     2641 gtccgccggg ccggtggccg agggcaacgt tggcggcggc acgggcatga tctgccacga
     2701 gttcaagggc ggtatcggca cggcctcgcg tgtcgtggcc acgcagagcg gtacgtggac
     2761 gatcggcgcg ctggtgcagg ccaaccacgg cgcgcgcgag gacttcc
//
LOCUS       DAOSMQ010000427         2799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_35962_length_2799_cov_4.627551, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000427 DAOSMQ010000000
VERSION     DAOSMQ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35962_length_2799_cov_4.627551"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..167
                     /locus_tag="PK829_12295"
     tRNA            95..167
                     /locus_tag="PK829_12295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Lys,seq:ttt)
     gene            212..1516
                     /locus_tag="PK829_12300"
     CDS             212..1516
                     /locus_tag="PK829_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU42037.1"
                     /translation="MSAPSRVALGNVPSSAAPRGDAAMNTENIVVDLRRMNRVLDWNP
                     ETGVIRVEPGVTLSELWQYILEDGWWPPVATGTSFTTMGGSAAMNVHGKNAYKAGPLG
                     DHILEFDLMLASGETITCSREANSDVFYAAIGGFGMLGIFTSLTIQMKRIYSGLLDVE
                     TTARGNLREMLDYFYENTGRSDYTVGWLDSLAGGRTLGRGDVHRANYLAPGDDPTPAQ
                     TLRLENQHLGPNLFGVVPRSIIWMFMWPMMRDIGVRVVNQGKFLMGNVSGVKRYRQPH
                     AQFHFLLDYVPDFKRAYSPGGLIQYQPFIPKETAEDAFGAILALCQRRGLPNYLTVLK
                     RHKPDDFLLSYAVDGFSMAMDFRVTNDNRQRMKWLTQELDEIVLAAGGRFYFAKDSTL
                     RPEVARAYLGDERIAAFHELKRRCDPDNLLQTDLWRRVFPDK"
     gene            complement(1592..2023)
                     /locus_tag="PK829_12305"
     CDS             complement(1592..2023)
                     /locus_tag="PK829_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42038.1"
                     /translation="MPAARYRPGQSFPIQFAWRLPGGEYLRAVFQADVLELAPSAEKY
                     VVRLSQLVAGREETAEGQPKPPAALSRDYWQLVGGLIGRRITLAYEADDGRALYMRLE
                     TLTGEHNYFTRYEDATVIARGIETRLQQLRARGAVDSTEEE"
     gene            complement(2099..>2799)
                     /locus_tag="PK829_12310"
     CDS             complement(2099..>2799)
                     /locus_tag="PK829_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOU42039.1"
                     /translation="SGGQRQRVAVGRAIVREPAVFLMDEPLSNLDAKLRVSARAEISK
                     IHQRLGTTFIYVTHDQVEAMTMGDRIAVLADGILQQCDTPRNLYNEPVNVFVAGFIGS
                     PSMNFFNGTLVNEEGRQFIDTGDFRVRIPEDRKEAFGPYSGKEVIMGIRPEHVHAPEF
                     APPSIKAEPVSATVEVVELLGHELHLYLNSGHNSFVGTVDPRFAVHTGNKIDIVFDMN
                     NVHLFDKATELAIR"
BASE COUNT          470 a          886 c          933 g          510 t
ORIGIN      
        1 ccgcgctcgc ggagatcgag gcggccgggg ccgaggtcgt ggggtccggc ggctgagcgt
       61 tcacgcccgc tggagcgaag taggctggac gggcgggccg ctagctcagt cggtagagca
      121 tcggactttt aatccgcggg tcgtgggttc gagcccctcc gggcccactt tcagatgttt
      181 cgtgacctcg gtcacacctg tcgagggccg cgtgtccgca ccgtcgcgcg tcgccctcgg
      241 caacgtgccg agttcggccg ccccgcgcgg cgatgcggcc atgaacaccg agaatatcgt
      301 tgtcgatctg cgacgcatga accgcgttct ggactggaac ccagaaaccg gcgtcattcg
      361 cgtggaaccg ggcgttaccc tgtcggagtt gtggcagtac atcctggaag acggctggtg
      421 gccgccggtg gcgacgggta cgagcttcac cactatgggc ggctcggcgg ccatgaacgt
      481 ccatggcaag aacgcctaca aggctggccc cctgggcgac catatcctgg agttcgacct
      541 gatgctggcc tccggcgaga cgatcacctg tagccgcgaa gccaacagcg acgtcttcta
      601 cgccgccatc ggcggcttcg gcatgctggg catcttcacc agcctgacga tacagatgaa
      661 gcgcatttac tccggtctgt tggatgtgga gacgaccgcg cgcggcaatc tgcgcgagat
      721 gctcgactac ttctacgaga acaccggccg ctccgactac accgtcggct ggctcgactc
      781 gctggccggc ggccggacgc tgggccgcgg cgatgtccac cgggccaact atctggcccc
      841 cggcgacgac cccacaccgg cccagacgtt gcgcctggag aaccagcacc tcggcccgaa
      901 cctctttggc gtcgtgcccc ggtcgatcat ctggatgttc atgtggccga tgatgcgcga
      961 tatcggcgtc cgcgtcgtca accagggcaa gttcctgatg ggcaatgtca gcggtgtcaa
     1021 gcgctatcgc cagccgcatg cgcagttcca cttcctgctc gactacgtgc ccgacttcaa
     1081 gcgcgcctac tcgccgggcg gcctcatcca gtatcagccg ttcattccca aggagacggc
     1141 cgaggacgcc ttcggcgcga tcctggccct gtgccagcgg cgcggcctgc ccaactatct
     1201 gaccgtgctg aagcggcaca agccggatga cttcctgttg tcctatgccg tcgatggctt
     1261 ttcgatggcg atggacttcc gcgtgaccaa cgataaccgg caacgcatga agtggctgac
     1321 gcaggaactg gacgagatcg ttctggccgc gggcgggcgc ttctatttcg ccaaagacag
     1381 cacgctgcgg ccggaagtcg cccgcgccta cctgggcgac gagcgcatcg ccgccttcca
     1441 cgagttgaaa cggcgttgcg atcccgacaa cttattgcaa acggacctgt ggcggcgcgt
     1501 tttcccggac aaatagtccg tcattcgtgc gccacaaacc acaacgcggc cggggtgtga
     1561 gtatcccggc cgcgtttttc gttagccggg cctactcctc ttcggtcgaa tcgaccgcgc
     1621 cgcgggcgcg caactgctgt agtcgcgtct cgataccgcg ggcaatgacc gtggcgtctt
     1681 cgtagcgggt gaagtagttg tgttcgcccg tcagcgtctc cagacgcata tagagggcgc
     1741 ggccgtcgtc ggcctcatag gccagggtga tgcgccggcc gatcagcccg ccgaccagct
     1801 gccagtagtc gcggctcagc gcggcgggcg gcttgggttg gccctcggcc gtctcctctc
     1861 gcccggccac gagctggctc aaccggacga cgtacttctc tgccgatggg gccagttcca
     1921 gcacgtcagc ctgaaagaca gcgcgcaggt actcaccgcc gggcagccgc caggcgaact
     1981 ggatggggaa ggattggccg ggccggtagc gggctgcggg catggggcgg gggagtggca
     2041 agggccgccg tcctgggggg cggcggccct ttagcggggt gatgtcatcg tcaacagact
     2101 agcggatagc cagttcggtg gccttgtcga acagatgaac gttattcatg tcgaagacga
     2161 tgtcgatctt gttgccggtg tggacggcaa aacgcgggtc gaccgtgccg acaaagctgt
     2221 tgtggccgga gttgaggtat agatgcaact cgtgccccag caactcgacg acctcgacgg
     2281 tggccgagac cggctccgcc ttgatgctcg gcggcgcgaa ttccggagcg tggacgtgtt
     2341 ccggacgaat gcccatgata acttccttgc cggagtaggg gccgaaggct tccttgcgat
     2401 cctcagggat gcgcacgcgg aaatcgcccg tgtcgatgaa ttggcggccc tcttcgttga
     2461 ccagcgtgcc gttgaagaag ttcattgacg ggctgccgat gaagccggcg acgaagacgt
     2521 tgaccggctc gttgtacagg ttgcgcggcg tgtcgcactg ctgcaagatg ccgtcggcca
     2581 gcacggcgat gcggtcgccc atagtcatgg cctctacctg gtcgtgggtg acatagatga
     2641 aggtcgtgcc caggcgctgg tgaatcttgc tgatctccgc gcgggcagag acgcgcagct
     2701 tggcgtccag gttcgacagc ggctcgtcca tgaggaacac tgccggctcg cggacgatgg
     2761 cccggccgac ggcgacacgc tgccgctggc cgccggaga
//
LOCUS       DAOSMQ010000428         2792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36076_length_2792_cov_4.600292, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000428 DAOSMQ010000000
VERSION     DAOSMQ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36076_length_2792_cov_4.600292"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PK829_12315"
     CDS             complement(<1..1519)
                     /locus_tag="PK829_12315"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOU42040.1"
                     /translation="MTWKRGLLGALVAVALLAGGVLVYRQFFGPASTTPGAAATPAPP
                     SAAIAVPNDGAVSAEGQIVPVRQANLVFSVSGVVAEYFVAEGESVAAGDRILRLDDTE
                     QQLAVERAQAAQTQAIANRTAAGNSLEAAQTNLEAVALDVTAAEVQLGLARAEPRPQE
                     IALSEAELALAEARLVQATAAQALVLEGAPDSRVRAAEAELRAAEARAIPSRLRLEQE
                     RLRDEPDADALAQAERDYNAALAAVAAAQQALDELAGGATAAQQSAAASGVSAAAAQR
                     DAAATELAALRAGQRPEEVAIAEARLAAARAAEAEAQARSLAADRAAQVAATEVTRVQ
                     EDLGAAFVALQQRTLVAPFDGTVADFPFSAGEVVGAGVPAAILADFSSWLVETTDLTE
                     LDVAEIATGAPAQVRADALPDLALSGVVTGIAPIAREVRGDTTYKVTVRLDDVVDAPL
                     RWGMTVFASFDEAAPAVLSAVARESGAVRAEGQLLPEQSAALSFQMPGRAILTGVQ"
     gene            complement(1569..>2792)
                     /locus_tag="PK829_12320"
     CDS             complement(1569..>2792)
                     /locus_tag="PK829_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU42041.1"
                     /translation="DMRRMDAIEWIDRENCRACVQAGITGSHLEERLAAEGFTCGHEP
                     DSVELSTLGGWIATNASGMKKNRYGNIEDIVESVTLVTPRGLIEQLMPMPRVSMGIEP
                     KHVAFGSEGNLGIITRAVIKIHRLPEVKRYGSYVFKDFRTGTAFLHDLAQAGVLPASI
                     RLVDNVQFRFGQALKGQPTSSDKLMGAAQKTFLFSVKGFDPHEIVAATIVMEGTAEEV
                     EHQAAMVKRYAARHGGVSGGSHNGQRGYMLTYAIAYIRDFLTDYHIIGETYETTVPWS
                     RIQEVCDAVAERATAMHRQFGLPGQAYVSPRITQLYHTGVCIYFTHGFSAKGAEAPDR
                     IFATIEHAMRETIMAHGGSISHHHGIGKLRRDFLSQTLTPASVELLREVKDGVDPNNV
                     FGIANNVFAPETNGH"
BASE COUNT          402 a          945 c          996 g          449 t
ORIGIN      
        1 cctggacacc ggtcaagatc gcgcgccccg gcatctggaa cgacagcgcc gccgattgct
       61 ccggcagcag ttgcccctcg gcgcggactg ccccgctttc gcgggccacg gcggacagca
      121 cggccggggc ggcctcgtcg aacgaggcga agacggtcat gccccagcgc aggggcgcat
      181 caacgacatc atcgaggcga acggtgacct tgtaggtcgt atcgccgcgc acctcgcggg
      241 cgataggagc aatgccggtc acgacgccgc tgagggccaa atctggcagg gcgtcggcgc
      301 gcacctgggc cggcgcgccg gtggcgatct cggccacgtc caactccgtc agatcggttg
      361 tctccaccaa ccagctgctg aaatcggcca ggatggcggc gggcacgccc gcgccgacga
      421 cctcgccggc ggagaaagga aaatcggcta ctgtcccgtc gaaaggggca accaaggttc
      481 gctgttggag ggcgacgaag gcggcgccca ggtcttcctg tactcgtgtc acctctgtcg
      541 cggctacttg agcagcccgg tcggccgcca gggagcgcgc ctgagcttcg gcctcggccg
      601 cccgcgccgc ggccagccgg gcctcggcga tggcgacctc ttccggccgc tggccggcgc
      661 gcaacgccgc caactcggtc gcggcggcgt cgcgctgggc cgcggcggcg ctgacgccac
      721 tggcggcggc gctctgctgg gcggccgttg ctccgccggc cagctcgtcc agcgcctgct
      781 gggccgcggc gacagccgcc agggcagcgt tgtagtcgcg ctcggcctgg gccagggcgt
      841 cggcgtctgg ctcgtcgcgc agccgctcct gctccaggcg caggcgcgac gggatggccc
      901 gcgcctcggc ggcgcgcagt tcggcctcgg cggcgcggac acgcgaatcg ggcgcgccct
      961 ccaggacgag cgcctgggcg gctgtggcct gaaccagccg cgcctcggcc aacgccaact
     1021 cggcctcgct cagggcgatc tcctgcgggc gtggttcggc gcgggccaga cccaactgaa
     1081 cttcggccgc ggtcacgtcc agcgcgacgg cctccagatt cgtctgggcg gcctccaggc
     1141 tgttgccggc cgcggtccgg ttggcgatgg cctgggtctg cgccgcctgg gcgcgctcca
     1201 cggccaactg ctgctcagtg tcatcaagac gcaggatgcg atcgcccgcg gcgacgcttt
     1261 cgccctcggc aacaaagtac tcggccacga caccggacac gctgaagacc agattggcct
     1321 gccgcacggg cacgatctgc ccctcggccg acaccgcgcc gtcattgggc acggcgatgg
     1381 ccgccgacgg cggcgcgggc gtggccgcgg cccccggcgt cgtggaggcc gggccgaaga
     1441 actgccggta gacgagcacg ccgccggcca gcagggcaac ggcgaccaaa gcgccaagga
     1501 gtccgcgttt ccaggtcata ggatgtttgc tgcgatggtt cggtcggggc gtcggtcggc
     1561 gcggttggtt agtggccgtt cgtttccggc gcaaagacgt tgttggcaat gccgaagacg
     1621 ttgttgggat cgacgccgtc cttgacttcg cgcaatagct cgacgctggc cggcgtcagc
     1681 gtctgcgaca gaaagtcgcg gcgcagcttg ccgatgccgt gatggtggct gatggagccg
     1741 ccgtgggcca tgatcgtctc gcgcatggca tgttcgatgg tggcgaagat gcggtcgggg
     1801 gcctcggccc ccttggccga aaagccgtgg gtgaagtaga tgcacacgcc ggtgtggtag
     1861 agctgggtga tgcgcggcga gacgtaggcc tggcccggca gcccgaactg gcgatgcatg
     1921 gccgtggccc gctcggcgac ggcgtcgcac acttcctgga tgcggctcca gggcacggtc
     1981 gtctcgtagg tctcgccgat gatgtggtag tcggtcagga agtcgcggat gtaggcgatg
     2041 gcgtaggtca gcatgtagcc gcgctggccg ttgtgggagc cgccggagac gccgccatgc
     2101 cgcgcggcat agcgcttgac catcgccgcc tggtgctcga cctcctcggc cgtaccttcc
     2161 atgacgatcg tcgcggcgac gatctcgtgc gggtcgaagc ccttgacgga gaagaggaac
     2221 gtcttctgcg ccgcgcccat cagcttgtcg ctgcttgtcg gctgcccctt cagcgcctgc
     2281 ccaaagcgga actggacgtt atcgaccagc cggatgctgg ccggcagcac gcccgcctgg
     2341 gccaggtcgt ggaggaaggc tgtgcccgtg cgaaagtcct taaagacgta ggagccatag
     2401 cgcttcacct ctggcagcct gtggatcttg atcaccgcgc gggtgatgat gcccaggttg
     2461 ccctcgctac cgaaggcgac atgcttcggc tcaatgccca tggacacgcg cggcatgggc
     2521 atgagttgct caatgaggcc acgtggcgtc accagggtga cgctctcaac gatgtcctcg
     2581 atattgccgt agcggttctt cttcatgccg ctggcgttgg tggcaatcca gccgcccagg
     2641 gtcgatagtt cgacgctgtc cggctcgtgg ccgcaggtga agccctcggc cgccagccgc
     2701 tcctccaggt ggctgccggt gatgccggcc tggacgcagg cgcggcagtt ctcccggtcg
     2761 atccactcga tcgcgtccat gcggcgcata tc
//
LOCUS       DAOSMQ010000429         2790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36109_length_2790_cov_4.443510, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000429 DAOSMQ010000000
VERSION     DAOSMQ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36109_length_2790_cov_4.443510"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="PK829_12325"
     CDS             <1..1509
                     /locus_tag="PK829_12325"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU42042.1"
                     /translation="ARVYLLGVLIFGLLALVIALPAGMAMAFLTTKQLLLLFNIDYVG
                     FRFSTRAVIWQVLSAILAPLLAALWPVMRGAAISVREALASYGLGGDFGSTRFDQTVE
                     RLAERVLPSPYTIALGNMFRRKGRLLLTQLVLVLAGTMFLMVLTLSASMTHTLDMELN
                     RRDYDLRLFFFQPHRSDRLVDLVSHYPGVVAAEPWYSVSGTVMREGEEVQDTGGLGAE
                     LFGIPAGSTFYQPLIISGRWLSPDDTGRVAVVSRETAEFNDLAVGDVITVDLGDLGQA
                     DFEVVGTYQAISPDVFSTDSIYAPATAVVDVTRRANRANQIVIRAADRSPAGIEALMS
                     GLDQYLKTYNVEVNPFFSRTNAEDRVYAYNTFSIVNQMLFSVAIVMGVVGGIGLMGSL
                     SISVVERTREIGVLRSLGGTSAVIMTMFVLEGVLQGLMSWLVAVPLAYLVARPVSALL
                     GQTIFRTDLDFAFNTGAVFIWLLAVLAIAFLSSLIPAHGASRISVRESLAYG"
     gene            complement(1672..>2790)
                     /locus_tag="PK829_12330"
     CDS             complement(1672..>2790)
                     /locus_tag="PK829_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42043.1"
                     /translation="GRRGAAGRTGAGSLAGGAARLWVFALGWTVLAALPSLLFLAPAY
                     VYGSPRLSYLPAVGVGLLWALPLLWLRRRLRVGGAALAVLTGVAAGALLWPARPFVVC
                     QLDFYADTSRMARQMGAIGASAPAGRELVFVNAPFFFSSTAARPDGCPSAFPWTPTGG
                     ILIPPYAQPRDFVRFNGGPDRAATGVTFAGYAPGWRTFGPEIDGDGLRAAAGESAVYV
                     FDLGRGNFVDLAAAWQPGNAPTASPLATFGDALALAGGTATAAAASETLNVRLDWRVL
                     AAPPFLPAVFVHVYDDGGVLVAQSDGPPAGGLVPLALWRPGDGLSDMRRLDLSALPPG
                     NYVVAVGVYNPADGLRLPAVAGGARLPDDVWWLQTFTK"
BASE COUNT          436 a          999 c          933 g          422 t
ORIGIN      
        1 gcccgcgtct acctgctggg cgtgctcatc ttcggcctgt tggcgttggt cattgccctg
       61 ccggcgggta tggctatggc ctttctgacc accaagcaac tgctcctgct gttcaacatc
      121 gactacgtcg gcttccgttt ctcgacgcgg gcggtgatct ggcaggtgct ctcggccatc
      181 ctggccccgc tgctggcggc gctgtggccg gtcatgcgcg gcgcggccat ctccgtgcgc
      241 gaggcgctgg ccagctatgg cctcggcggc gacttcggct ccacccgctt cgaccagacc
      301 gtcgagcgcc tggccgagcg cgtcctgccc tcgccctaca ccatcgccct gggcaacatg
      361 tttcgccgca aggggcggct gctgctgacg cagcttgttc tcgtgctggc ggggacgatg
      421 ttcctgatgg tcctgacgct gtcggcgtcg atgacccaca cgctggacat ggagttgaac
      481 cgccgcgact acgacctgcg cctgttcttc ttccagcccc accgctccga ccggctggtc
      541 gacctggtca gccactaccc cggcgtcgtg gccgccgaac cgtggtactc ggtcagcggc
      601 acggtgatgc gcgaagggga ggaagtgcag gacaccggcg gcctgggggc ggagttgttc
      661 ggcatccccg ccggcagcac gttctaccag ccgctcatca tcagcgggcg ctggctgtcg
      721 ccagacgaca ccggccgcgt cgccgtcgtc agccgcgaga cggccgagtt caacgacctg
      781 gccgtcggcg acgtcatcac cgttgatctg ggcgacctgg gccaggccga ctttgaggtc
      841 gtcggcacgt atcaggccat ctcgcccgat gtgttcagca ccgactcgat ctacgcgccg
      901 gccacggccg tcgtcgacgt cacccgccgc gccaaccgcg ccaaccagat cgtcatccgc
      961 gccgccgacc gcagcccggc cggcattgag gcgctcatga gcgggctgga tcaatacctg
     1021 aaaacctaca acgtcgaggt caatcccttc ttcagccgca ccaacgccga agaccgcgtc
     1081 tacgcctaca acactttcag catcgtcaac cagatgctct tcagcgtggc cattgtcatg
     1141 ggcgtcgtcg gcggcatcgg cctgatgggg tcgctgtcga tcagcgtcgt cgaacggacg
     1201 cgcgagatcg gtgtgctgcg ctcgctgggc ggcacgtcgg cggtcatcat gacgatgttc
     1261 gttttggagg gggtgttgca agggctgatg agctggctgg tggccgtgcc gctggcctat
     1321 ctcgtcgccc ggccagtctc ggccctgctg gggcagacga tcttccgcac cgacctcgac
     1381 ttcgccttca acaccggcgc ggtcttcatc tggctgctgg ccgtgctggc catcgccttc
     1441 ctgtcctcgc tcatccccgc ccacggggcc agccgcatca gcgtccgcga gagcctggcc
     1501 tacggctgaa acgaaaaagc gcccagaaac gcgtacagcc ccggccagga gggggaaccg
     1561 gggctgcacg cattcttggc gacttcattt aagagtatag acccgcttcc taaattagat
     1621 gtgagccaga cgtgagcgga tgctaagcac ccattcgggt cttctctgga gctatttggt
     1681 aaaggtttga agccaccaca cgtcgtcggg caggcgcgcg ccaccggcga cggccggcaa
     1741 gcgcaacccg tcggccgggt tgtagacacc cacggcaacg acgtagttgc ccggcggcag
     1801 cgcgctcagg tcgagccgcc gcatgtcgct caggccgtcg cccggccgcc acagcgccag
     1861 cgggaccagc ccgccggccg gcgggccgtc gctctgggcc accaacacgc cgccatcgtc
     1921 gtagacgtgg acaaagacgg ccggcaggaa gggcggcgcg gccagcacgc gccagtcgag
     1981 acgcacattc agtgtctcgc tcgccgctgc cgctgtcgcc gtcccgccgg ccagcgccag
     2041 cgcgtcgcca aaggtcgcca gtggcgaggc agtgggtgcg ttgcccggct gccaggcggc
     2101 ggccaggtca acgaagttgc cgcggccgag gtcgaagacg tagacggcgc tctcgcccgc
     2161 cgcggcccgc aagccgtcgc cgtcgatctc cggcccgaac gtccgccagc cgggcgcata
     2221 gccggcgaag gtcacgcccg tcgccgcccg gtctggcccg ccgttgaagc gcacgaagtc
     2281 gcgcggttgg gcgtagggcg ggatgaggat gccgccggtg ggcgtccacg ggaaggcgct
     2341 ggggcagccg tcggggcggg cggccgtgga actgaagaag aagggcgcgt tgacgaagac
     2401 gagttcgcgc ccggcggggg cggacgcgcc aatggccccc atctggcggg ccatgcggct
     2461 ggtgtcggcg tagaagtcaa gctggcagac gacaaacggc cgcgccggcc acagcagcgc
     2521 ccctgcggcc acgcccgtca gcacggccaa cgccgccccg cccacgcgca gccgccgccg
     2581 caaccacaac agcggcagcg cccagagcag gccgacgccg acggccggca gatagctcag
     2641 ccgcgggctg ccgtagacgt aggccggggc gaggaacagc agcgacggca gcgccgccag
     2701 cacagtccag ccaagcgcga aaacccacag ccgcgccgcc ccgcccgcca ggctgccagc
     2761 cccagtacgg ccagcagccc cacggcggcc
//
LOCUS       DAOSMQ010000430         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36379_length_2774_cov_3.833395, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000430 DAOSMQ010000000
VERSION     DAOSMQ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36379_length_2774_cov_3.833395"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK829_12335"
     CDS             complement(<1..195)
                     /locus_tag="PK829_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42044.1"
                     /translation="MSNATTVERVRAHLAALVRWPGGRPVGSPANYAAQDTIAAALAA
                     AGYEVEQQDFVCLTWRLEEAG"
     gene            complement(205..720)
                     /locus_tag="PK829_12340"
     CDS             complement(205..720)
                     /locus_tag="PK829_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42045.1"
                     /translation="MKRVYGLLLVLALVMLAGCGLVSTGESAAEKATEIATFDIPAGY
                     TPDVGMDLAGTVMVGYSNAAENGHIYLIQAPASSNLSQAQLEQSLRDALNSSQDATVD
                     TVDLEEMAMTIRGEEVTATVGTATGQNDTTMRVLTVPFTGNGGPALLLIQTAEASWDQ
                     ATIDDFIASFR"
     gene            complement(783..2471)
                     /locus_tag="PK829_12345"
     CDS             complement(783..2471)
                     /locus_tag="PK829_12345"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOU42046.1"
                     /translation="MLRKLALVLLLIALFFSLAAQSDKSYSADRFDVDVAAQADRSLL
                     VTETVAFRFVGGPFSFVFRELPTDHTDGITNIVAGVDGVPWPEGTGPGQVEITGRNPI
                     VITWHLSPTSDVVQNFTLSYQVLGVVRRGEQADVLDWQALPDEYEYDIASSTVTVNYP
                     TGMTLQGEPEVLAGKADFASSGNRVVFTQGNLSAGDPLVVRLSFPPGSFAGAPPAWQT
                     QREAQNRWSWAWIAAAVATLIGGLLAIFAGMRGFSQPTRKPDALLHKPPVDLPPALAG
                     WLFNQSITWPHGLATLLDLAGRGVVAIEEATEKKWYRSADFLITLLKQPADARPHERA
                     LLDLLFTDRSSAPQNAIRMSDMGRLITSSRWKTYTESLETEADAQGLISAEAKQRRNR
                     FLTWGVMLLFLAGAIMLVIFLVGSLFGWWPFLLAAAVALLALVAFIAGAAVSPLSDRG
                     TELVETFAPFRRFIEQVSRGKIEVPDPAYYEAHLPYATAFGYAVPWVKKQVDEGYSEV
                     PAYFHALNADDGAHVAAWLVAVTAATNSGGSAAPASAAGAAGAGAAGGGASGAG"
BASE COUNT          486 a          905 c          933 g          450 t
ORIGIN      
        1 tccggcctct tccagccgcc aggtcagaca gacgaagtcc tgttgttcca cctcgtagcc
       61 ggcggcggcc agcgcggcag cgatggtgtc ctgggcggcg tagttggcgg gcgagccgac
      121 cgggcggccc cccggccagc gcaccagcgc cgccaggtgc gcgcgaaccc gttcgactgt
      181 cgtcgcgtta ctcatggcca ttgattagcg gaacgaagcg atgaaatcat cgatggtcgc
      241 ctggtcccaa cttgcctcgg ctgtctggat cagcagcagc gccggcccgc cgttaccggt
      301 aaagggtaca gtcaggacgc gcatggtcgt atcgttctgg ccggtggccg tgcccaccgt
      361 ggcggtcact tcttcgccgc gaatggtcat agccatttcc tccagatcga ccgtgtccac
      421 cgtggcatcc tgggacgagt tcagcgcgtc tcgcaaggat tgctccaatt gcgcctggct
      481 cagattggac gacgcgggcg cctgaatcag gtagatgtgg ccattttcgg cggcgttgct
      541 atagccaacc atgaccgtgc cggccagatc catgcccacg tcgggggtat agccggcggg
      601 aatatcgaac gtggcgatct cggtcgcttt ttcggcggcc gattcgcccg tgctaaccag
      661 accgcagccg gccagcatca ccagagccag caccaacaac agaccataca ctcgcttcat
      721 gacagacctc caatccttcc tgagaacaga caacagctct ttatcgcacc cgtgggatgc
      781 gcttaacccg cgccgctggc cccaccgccg gccgccccgg ccccggctgc gcccgcagcc
      841 gaagcgggcg cagccgaccc gccggagttc gtggccgctg tgacggcgac caaccaggcg
      901 gcgacatgag cgccgtcgtc cgcgttgagg gcgtggaagt acgccggtac ttcgctgtaa
      961 ccctcatcaa cctgcttctt aacccagggc acggcatagc caaaggcggt ggcatagggc
     1021 aagtgcgctt cgtagtacgc cgggtcgggc acttctatct taccacggga gacttgctcg
     1081 atgaaacggc ggaaaggcgc aaaggtctcg accagttccg tgccgcggtc ggacagcggc
     1141 gagacagccg cgccggcaat gaacgcgacc agggccagca gcgccacggc ggcggccagc
     1201 aaaaagggcc accagccaaa cagagagcca accaggaata tcaccagcat gatcgcgccc
     1261 gcgaggaaca agagcatcac gccccaagtc aagaagcggt tgcgccgctg cttggcctcg
     1321 gcgctaatga gaccctgggc gtcggcttcg gtttccaggc tctcggtgta ggtcttccag
     1381 cgcgaggagg tgatcagccg gcccatgtcc gacatcctaa tggcgttttg gggcgcgcta
     1441 gagcggtcgg tgaagagcag gtcgagcagg gcgcgctcgt gggggcgagc gtcggccggt
     1501 tgcttcagca gcgtgatgag gaagtcggcc gaccggtacc acttcttctc ggtggcctcc
     1561 tcaatggcca cgacgccgcg gccggccagg tcgagtagcg tcgccaggcc gtggggccag
     1621 gtgatggact ggttgaacag ccagccggcc agggccggcg gcaagtctac gggcggcttg
     1681 tgcagcagcg cgtcgggctt gcgtgtcggc tggctaaagc cgcgcatgcc ggcgaagatg
     1741 gccagcagtc caccgatgag ggtggcgacg gcggcggcga tccaggccca tgaccagcgg
     1801 ttctgggcct cccgctgtgt ctgccaggcc ggcggcgcgc cggcgaagct gcccggcgga
     1861 aaggagaggc gcacgaccaa cggatcgccg gccgacaggt tgccctgggt gaagacgacc
     1921 cgattgccgc tgctggcgaa gtcggccttg ccggccagca cttccggctc gccttgcagg
     1981 gtcatgccgg tcgggtagtt gaccgtcacg gtgctgctgg cgatgtcgta ctcgtactcg
     2041 tccggcagcg cttgccagtc gagcacgtcg gcctgttccc cgcgccggac gacgccgagc
     2101 acctggtagg acagggtaaa gttctgcacg acgtcggacg taggcgacag atgccaggtg
     2161 atgacaatag gattgcggcc ggtgatctcg acctgcccgg ggccagttcc ttccggccac
     2221 ggcacgccgt ccactccggc gacgatgttg gtgatgccgt cggtgtggtc ggtcggcaat
     2281 tcgcggaaga cgaagctgaa cggccccccg acaaaacgga aggccacggt ctccgtaacg
     2341 agcaacgagc ggtcggcctg cgcggccaca tcaacgtcga agcggtcggc gctgtagctt
     2401 ttgtcgctct gggcggccag cgagaagaag agggcaatga gcaatagtac aagggcgagc
     2461 ttgcgaagca ttgtgtttac tccactcctg tgctaggcga tcgggcggcc ccggctgggc
     2521 acgcggctga tcatcatgac aacatcccat cccctctacg gagatcgctc atgcgggttg
     2581 cgccggcgag cgcggcctca ggcgctcgct ccgctgtcag cgggccgcca ggacggcccg
     2641 cgccgcgttg tagcccggcg cgccagtcac gccgccgccg gggtgcgccc ccgcgccaca
     2701 gaggtacagg ttacgcactg gcgtctcgta gcggctccag ccgggaatgg gccgcatgac
     2761 cagcatctgg tcca
//
LOCUS       DAOSMQ010000431         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36473_length_2769_cov_4.078482, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000431 DAOSMQ010000000
VERSION     DAOSMQ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36473_length_2769_cov_4.078482"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK829_12350"
     CDS             <1..386
                     /locus_tag="PK829_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42047.1"
                     /translation="SQFAGRYAAYAGNAPTFVEKARLYPGAVFVVGFDTAARVIQPRY
                     YGDSQAGMLAALDEVRARGNRFLVAGREGADGAFHELAELALPAGYAALFAPIPASLF
                     HADVSSTELRAELKAEADAEAAARR"
     gene            complement(403..1074)
                     /locus_tag="PK829_12355"
     CDS             complement(403..1074)
                     /locus_tag="PK829_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42048.1"
                     /translation="MRARKLSVLAVLLLALAACAGLRGVESGDITAQAEETQSVIVPG
                     VPTLTVNHFAGDLTIRDGAADKITAHVTRQSTLSDAAAAEASLADITVAFSQSGSDVT
                     LDVEVPKKLTNEINDLLAIVELEVPPGTTLFLNQGGGNVVVEQPSGDVTIDAGAGDFT
                     VTLPADAAFRLIVAGGVAKVASDFEGVPSGGLAADIDTTVGDNPTQTLTFNVGAGQVN
                     LRHAD"
     gene            complement(1085..1519)
                     /locus_tag="PK829_12360"
     CDS             complement(1085..1519)
                     /locus_tag="PK829_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOU42049.1"
                     /translation="MTQPIPASHSDLLARPVHAVLTTLMPDGQPQSCLMWCDSDGECA
                     RVNTTLERQKGHNLSRDPRATLLLMDPDDTSRFMELRGEMELLTEGALEHLDALTRKY
                     TAHPQFYGYVYPEQQRDRETRVIGRLIAAKITLDAIHRERKD"
     gene            complement(1524..1949)
                     /locus_tag="PK829_12365"
     CDS             complement(1524..1949)
                     /locus_tag="PK829_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOU42050.1"
                     /translation="MSADIPAEFLDLVDGPPVAALSTLMPNGGPQTTAVWCDFDGAYV
                     RVNTMRGFRKERNMRLNPRVTLLCFDPREPLRYVEVRGVVAEMTEAGAREHLDGLSLR
                     YTGLTPYFGGCVPAELAATETPVLCRIRPVHVVCLDARE"
     gene            complement(1952..2572)
                     /locus_tag="PK829_12370"
     CDS             complement(1952..2572)
                     /locus_tag="PK829_12370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU42051.1"
                     /translation="MTDPAALHNAYAADYDAQVAAYESFIAEALFGLCFAFVRPGERL
                     LDVGIGSGLAAAPFARVGLRVSGMDFAAAMLDLCRAKGIAEDLRQHDLQRAPWPYATA
                     AFDHAICCGVCHFVPTLESTFSEARRVLRPGGIFAFTTKWPDAPLPPGVPFARVAAGE
                     LDVFSHAPEHVQSRLDAAGFTCLRRLRCFVGQDIFGVWIARRGKGE"
BASE COUNT          438 a          899 c          973 g          459 t
ORIGIN      
        1 tgagccagtt cgccgggcgc tacgcggcct acgccggcaa cgcgccgacg ttcgtcgaaa
       61 aggcgcgcct ctatccgggg gctgtctttg tcgtcggctt cgacacggcc gcgcgcgtca
      121 tccagccgcg ctactacggc gacagtcagg cgggcatgct ggccgcgctg gacgaagtgc
      181 gcgcgcgggg caaccgcttt ctggttgccg ggcgtgaggg ggccgatggc gcgttccacg
      241 aactggccga gctggcgctg ccggccggct atgcggccct gttcgcgccc atccccgcga
      301 gcctgttcca cgccgacgtg tcctcgaccg aactgcgtgc cgaactaaaa gccgaggccg
      361 acgccgaagc cgccgcgcgg cgctaggggt cgcggccgtc gtttagtcgg cgtggcgcag
      421 attcacctgg cccgcgccga cgttgaaggt cagcgtctgc gtcgggttgt cgcccacggt
      481 cgtgtcgatg tcggcggcca gaccgccaga gggcacgccc tcgaagtcgc tggccacctt
      541 ggccacgccg ccggccacaa tgagccggaa ggcggcgtcg gccggcaggg tcacagtgaa
      601 gtcgcccgcg ccggcatcga tggtcacgtc gcccgacggt tgctcaacga cgacgttgcc
      661 gccgccctgg ttgaggaaca gcgtcgtgcc cggcggcact tccagttcga cgatggccag
      721 caggtcgttg atctcgttcg tcagcttttt gggcacctcg acgtcaaggg tgacgtcgct
      781 gccgctctgg ctgaaggcca cggtgatgtc tgccagactg gcctcggccg cggcggcgtc
      841 gctgagggtg ctctgccggg tcacgtgggc ggtgatcttg tcggccgcgc cgtcacggat
      901 ggtcagatcg ccggcgaagt gattgaccgt cagcgtgggc acgccgggga cgatgacgct
      961 ttgcgtctcc tcggcttggg cggtaatgtc gccgctctcg acgccgcgca ggccggcgca
     1021 ggcggccagt gccagcagca gaacggcaag aacagatagc ttcctggctc tcatcgtaaa
     1081 cctcctaatc tttcctttcg cggtggatgg cgtccagcgt gatcttggcg gcgataagac
     1141 ggccaatcac gcgggtttcg cgatcgcgct gttgctctgg atagacgtag ccgtagaact
     1201 gtgggtgggc ggtgtacttg cgcgtcaggg catccagatg ctcaagcgcg ccctcggtca
     1261 gcaattccat ctcgccccgc aactccatga agcggctggt gtcgtcgggg tccatgagca
     1321 gcagggtggc ccgcgggtcg cggctcaggt tgtggccctt ctgacgctcc agagtggtgt
     1381 tgacgcgcgc gcattcgccg tcgctgtcgc accacatcag gcacgattgc ggctggccgt
     1441 cgggcatgag cgttgtcagc acggcgtgga cgggccgggc cagcaggtcg ctgtgggagg
     1501 cggggatggg ttgggtcatg gggtcactcc cgcgcgtcca ggcacacaac atgcaccggc
     1561 cggatgcggc aaaggacggg cgtctccgtg gccgccagtt cggccggcac acagccgccg
     1621 aagtagggcg tcaggccggt gtaacgcaga gataggccgt cgaggtgctc ccgcgcgccg
     1681 gcctcagtca tctccgcgac gacgccgcgc acctccacat agcgcagcgg ctcgcgcggg
     1741 tcgaagcaca gcagggtgac gcgcgggttg agccgcatgt tgcgctcctt gcgaaagccg
     1801 cgcatcgtgt tgacgcgcac gtatgcgcca tcgaagtcgc accagacggc tgtggtctgt
     1861 ggcccgccgt tgggcatcag cgtgctcagc gcggccaccg gcgggccgtc taccaggtca
     1921 agaaactcgg ctggaatgtc ggcgctcatg gtcactctcc tttgccccgg cgggcgatcc
     1981 agacgccaaa gatatcctgc ccgacaaagc agcgcagccg ccggagacag gtgaagccgg
     2041 cggcgtctaa cctggactgg acgtgctccg gcgcatggct gaacacgtcc aactccccgg
     2101 ccgccacgcg ggcaaagggc acgcccggcg gcagcggcgc gtcgggccac ttggttgtga
     2161 aggcgaagat gccacccggc cgcagcacac gccgcgcctc gctgaaggtg ctctccagcg
     2221 tgggcacgaa gtggcagacg ccgcagcaga tggcgtggtc gaaggcagcg gtggcgtagg
     2281 gccagggcgc gcgttgtaag tcgtgctgcc gcaggtcttc agcaatgccc ttggcgcggc
     2341 aaaggtcaag catggccgcg gcgaaatcca tgcccgacac gcgtagcccg acgcgggcaa
     2401 agggcgcggc ggccagcccg ctgccgatgc ctacgtccag cagccgctcg cccggccgga
     2461 cgaaggcgaa gcacagcccg aagagcgcct cggcgatgaa actctcgtag gcggccacct
     2521 gcgcgtcata gtcggcggcg taggcgttgt ggagggcggc cgggtcggtc atgaagaggt
     2581 cacgtcctgg gcctgaaccg atcgttgact gaatgcttca gtttaccatt tgagatcgct
     2641 cgctgtccag acaggagttg tcgccatcgg ggctttgcca aagtcgtcga gcgactattg
     2701 acttgacagg tgcgcggcca atccaggaga agaccggtct ccgttctagt ctagccgcgc
     2761 caacaactc
//
LOCUS       DAOSMQ010000432         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36521_length_2766_cov_5.122833, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000432 DAOSMQ010000000
VERSION     DAOSMQ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36521_length_2766_cov_5.122833"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /gene="alaS"
                     /locus_tag="PK829_12375"
     CDS             complement(<1..1420)
                     /gene="alaS"
                     /locus_tag="PK829_12375"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU42052.1"
                     /translation="MRPTTSKEIRQAFLDYFNELKHEIVPSAPLPQKDNPTLLFTNAG
                     MNQFVDVFLGKEKRPYKRATTAQKVMRVQGKQNDLENVGPSARHHTFFEMLGNFSFGD
                     YFKREAIDYAWTFLTGVMELEPERLFATVYTDDDEAAALWQRYLPAERILRFGKKDNF
                     WQMGDLGPCGPCSEVHYYSGDMATITPRGVNNDDYPDYVEVWNLVFMQYDAQPDGTLV
                     PLPHPSVDTGMGMERLVRVIQGVPSNYETDLFTPALARIQALLGDSDAQRAEKYVGYR
                     VIADHGRAATFLIADGVQPGNTGAGYVLRMIIRRAARFGRKIGFHEPFLGHVAQVYID
                     EMGEAYPELRLRRDLILYTLEREETRFNRTLDSGLLQLDRILDELHSRGETVVPGDVA
                     FALYATHGLPLELTRDVALEQHGMSVDEDGYTAAREQHALASGSGAFANYVVGQSVYG
                     DMLHSLVNTGQLDDSGVDYDPYT"
     gene            complement(1690..2547)
                     /locus_tag="PK829_12380"
     CDS             complement(1690..2547)
                     /locus_tag="PK829_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HOU42053.1"
                     /translation="MNSDRIGLLHPGEMGISVAATLQNGGQTVYWASTGRSAPTRARA
                     EQHSLRDAGTLAALCAACPVIVSVCPPHAAEAVAADVLASGFRGLYLDANAISPQRAQ
                     RLAQSFASAGATFVDGGIIGGPAWQPDTTWLYLSGPAAAEAATLFAAGPLATIVLDGP
                     AGQASALKMVYAAWSKGTTALLAAVLATAEALDVRGHLETQWARDGSELATTAAPRAR
                     RVTRKAWRFAGEMDEIAATFGAAGLPDGFHRAAADVYGRLAAFKGRDEPPTLAEVLRA
                     LLPTTGLDN"
BASE COUNT          443 a          864 c          962 g          497 t
ORIGIN      
        1 cggtgtaggg gtcgtagtcc acgccgctgt cgtccagttg gccggtgttg accagggagt
       61 ggagcatgtc gccgtagacg ctctggccga cgacgtagtt ggcgaacgcg ccgctgccgc
      121 tggccagggc atgctgctcg cgggccgcgg tgtagccgtc ctcgtccaca ctcatgccgt
      181 gctgctccag ggccacgtcg cgcgtcagtt ccagcggcag gccgtgggtg gcgtagaggg
      241 cgaaggccac gtcgccgggt acgaccgtct cgccgcggct gtgcagctcg tcgaggatgc
      301 ggtcgagttg cagcaggccg ctgtcgagag tgcggttgaa gcgggtctcc tcgcgctcca
      361 gcgtgtagag gatgaggtcg cgccgcagcc gcaactctgg gtaggcttcg cccatctcgt
      421 cgatgtagac ctgggcgacg tggcccagga acggctcgtg gaagccgatc ttgcggccga
      481 agcgcgccgc ccggcggatg atcatccgca ggacgtagcc cgcgccggtg ttgcccggct
      541 ggacgccgtc ggcgatgagg aaggtcgccg cgcggccgtg gtcggcgatg acgcgatagc
      601 cgacgtactt ctccgcccgc tgcgcgtcgc tgtcgcccaa cagcgcctgg atgcgggcca
      661 gcgctggcgt gaacaggtca gtctcgtaat tgctgggcac gccctggatg acgcgcacca
      721 gccgctccat gcccatgccg gtatcgaccg acgggtgggg taggggcacg agcgtgccgt
      781 cgggctgcgc gtcgtactgc atgaagacca gattccagac ctcgacatag tccggatagt
      841 cgtcgttgtt gacgcccctg ggggtgatgg tggccatgtc gccggagtag tagtggactt
      901 ccgagcaggg gccgcacggg ccgaggtcgc ccatctgcca gaagttgtcc ttcttgccga
      961 agcgcaggat gcgctcagca ggcaggtagc gctgccacag cgcggcggcc tcgtcgtcgt
     1021 cggtgtagac tgtggcgaag aggcgctccg gctccagttc catgacccca gtcagaaaag
     1081 tccaggcgta gtcaatggcc tcgcgcttga agtagtcgcc gaaggagaag ttgcccaaca
     1141 tctcgaagaa ggtgtggtgg cgggccgagg ggccaacgtt ctccaggtcg ttttgcttgc
     1201 cctgcacgcg catgaccttc tgggcggtgg tggcgcgctt ataggggcgc ttctccttgc
     1261 ccaggaagac atcgacgaac tggttcatgc cggcgttggt gaacaacagc gtcgggttgt
     1321 ccttttgcgg cagcggcgcg ctggggacga tctcgtgctt aagctcgttg aagtagtcga
     1381 gaaaggcttg tcgaatttcc ttactggttg tcggtctcat ggttactccg ggcggatttt
     1441 agcagataac ttttagacct gacaggtgtt ggcgttggcc gccaacgcca acacctgtca
     1501 ggtctggccg gtaaaaacaa aaaccgccag accgagtact cagtctggcg gttgccggtt
     1561 cgctgattgt gtctacgcgt caagcaacgt ccccgaccag actgagtgtc tggccggcgg
     1621 cggcgaagcg aacgtacttg cacgggtagc cgaacatgac agtactatag cagcagaggg
     1681 gagccactgt caattgtcta acccggtagt tggcagaagt gccctcaaaa cctcagcgag
     1741 cgtcggcggc tcgtctcgcc ccttgaaggc ggccagccgg ccgtagacat cggccgcggc
     1801 gcggtggaag ccatccggca ggccggccgc gccgaacgta gccgcgatct cgtccatttc
     1861 gccggcaaat cgccacgcct tacgcgtgac gcgccgggca cggggcgcgg ctgtcgtggc
     1921 cagttcgctg ccgtcgcgcg cccactgcgt ttccaggtgc ccgcgcacgt ccagcgcctc
     1981 ggccgtggcc agtacggcgg cgagaagggc tgtcgtgcct ttgctccagg cagcgtagac
     2041 catcttcagc gccgaggcct ggcccgctgg cccgtctagc acgatcgtcg ccagcggccc
     2101 ggcggcgaat agggtggctg cctcggccgc ggccgggccg gacaggtaga gccaggtggt
     2161 gtccggctgc cacgccggac caccgatgat gccgccatcg acgaaggttg cgccagcgct
     2221 ggcgaagctc tgggccaggc gctgtgctcg ctgcggcgaa atggcattgg cgtccaggta
     2281 aaggccgcgg aagccgctgg ccagaacgtc ggcagcgacg gcctcagccg cgtggggcgg
     2341 gcagacgctg acgatgaccg ggcaggcggc gcagagcgcg gccagcgtgc cggcgtcgcg
     2401 cagggagtgc tgctcggcgc gggcgcgcgt cggcgcgctg cggccggtgg aggcccaata
     2461 gacggtctgg ccgccgttct ggagcgtggc ggcgacggag atacccatct cgccggggtg
     2521 gagaaggcca atgcggtctg agttcattct gttatctccc tgttggcttg aaccgtgtcc
     2581 aagcatactg gctttgtttg gcgcggcaat catcgtttgg cgtggcgcca aatctcgcgt
     2641 acgattcggc ccacaaccct gggtcagcca gtatccagta tatggaaacg caaatgatca
     2701 ttctcgaaac gccccgcctc ctcctccgcc accccgtccc cgacgacctc gatgccctct
     2761 atgccc
//
LOCUS       DAOSMQ010000433         2759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36657_length_2759_cov_5.113905, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000433 DAOSMQ010000000
VERSION     DAOSMQ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36657_length_2759_cov_5.113905"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="PK829_12385"
     CDS             <1..1433
                     /locus_tag="PK829_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42054.1"
                     /translation="SNYPPLFHVVTVPLVWLFGPQYWTGRLVSFLGTLLTAAAIGYAV
                     GRGQPRGRDHDPGRARGRWWLAALSGLAFLASNYVYHVGPLFRQHMFMVMFETLAVVA
                     LAVVIEREESDGRRRTLGLLGVMLLLLAAGYTKQLAYATVIAVFLFLFLRGPWRAVRW
                     AVPFALVTGLLFLGINVATHGQWMLNTITANLNPFVPGQAEGLFRQWFGLHIVLVTAA
                     ALYAIYQLYATRLSIYAIWFVVATVNSVTAGKWGAGESYFATAIAAACILSGLAFDHW
                     LSWSTARRWPTPRMAAWAAVLLPSLIALLYLWQARQVFHMPTHTPALAAVARALGQPD
                     AVMIAPQTSCSLPRPPEAIPYVDAVGATLLGRPPTAADTRAGQQIAALIAQGDTAAFS
                     EDAGFNLYLGRDVVTNPTQLLNLYTNNAVDLTEMLAMLDAQAFDTVVLRAQFYPSPVL
                     DVIGQRYETTDLVQMNGFVYCIMRPR"
     gene            1430..2173
                     /locus_tag="PK829_12390"
     CDS             1430..2173
                     /locus_tag="PK829_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOU42055.1"
                     /translation="MNQYRVELPVFNGPLDLLLHLIEREELDITAVSLVQVTGQYLEQ
                     VRQLGDEQLEGLIDFISIGARLLLIKSRALLPRPPVLPGDEVEEEDPTEALLRQLRAY
                     KRFKTAAQWLDGRQRRGLRTYLRVAPPPQLEGHLDLTGVDVNTLLGALQAVLARTEVR
                     ESALSVAQPRTLTIEDQLRRLRARLGRGRAFLFNDVLVNPRDRTEIAVTLLALLELIK
                     RREAQASQSYLFGPIEVTVAGPEADAPAE"
     gene            complement(2184..2594)
                     /locus_tag="PK829_12395"
     CDS             complement(2184..2594)
                     /locus_tag="PK829_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42056.1"
                     /translation="MAKHAPNPDIYISTEWADAGYDCDHCGGRILRRTDHETGLRDHT
                     CLQCEQCRCQWTLARHPLRVGTTPACRAAQRRRAKAVETLGMLPRWMIIVLAVVVVLA
                     LARFGGVFLRLGAPAVLLVVVAAVVTYRRRRDAR"
     gene            complement(2575..>2759)
                     /locus_tag="PK829_12400"
     CDS             complement(2575..>2759)
                     /locus_tag="PK829_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42057.1"
                     /translation="EHIYHAALLDPHTAAELAPDEIYALVDDLLAAHGDWLPAYHPDE
                     RYAAPGLAPTNGQARS"
BASE COUNT          424 a          949 c          894 g          492 t
ORIGIN      
        1 cgtccaacta cccgcccctg ttccacgtcg tcaccgtgcc gctggtgtgg ctgttcgggc
       61 cgcagtactg gacggggcgg ctggtcagct ttctgggcac gctactgacg gcggcggcca
      121 ttggctatgc cgtgggccga gggcaaccgc ggggccggga ccacgacccc ggccgtgccc
      181 gtggccgttg gtggctggcg gccctatcgg ggctggcctt cctggcgtcg aactacgtct
      241 accacgtcgg cccgcttttt cggcagcaca tgttcatggt catgttcgag acgctggcgg
      301 tggtggcgct ggcggtggtc attgagcgtg aggaaagcga tggccgccgc cgcaccctcg
      361 gtctgctggg cgtcatgctc ctgctcctcg ccgctggcta cacgaaacaa ctggcctacg
      421 ccaccgtcat cgccgtcttc ctcttcctct tcctgcgcgg cccgtggcgg gccgtccgtt
      481 gggccgtgcc ttttgccctc gtcaccggcc tgctctttct gggcatcaac gtcgccaccc
      541 acgggcagtg gatgctcaat actatcaccg ctaacctcaa tccctttgtg cctggccagg
      601 ccgagggact gttccggcag tggttcgggc tgcatatcgt cctcgtgacc gcggcggcgc
      661 tctacgccat ctaccaactc tacgccacac gcctgtcgat ttatgccatc tggttcgtcg
      721 ttgccacggt caatagcgtc acggccggta agtggggcgc gggcgagtcc tacttcgcca
      781 cagccatcgc cgctgcctgt atcctctccg ggctggcctt tgaccactgg ctgagttgga
      841 gcacagcgcg gcgctggccc acgccacgga tggctgcctg ggccgcggtg ctgctgccgt
      901 cgctcatcgc cctgctttac ctctggcagg cacgacaggt cttccatatg cccacgcata
      961 cgccggcgct ggccgccgtc gcccgcgccc tgggacagcc agacgcagtg atgatcgccc
     1021 cgcagacctc gtgttcgctg ccccggccgc cggaggccat tccctacgtc gatgctgtgg
     1081 gggcgacgct gctgggccgc ccgcccacgg cggccgatac ccgcgccggc caacagatcg
     1141 ccgccctcat cgcccagggc gacaccgccg ctttcagcga agacgccggt ttcaacctct
     1201 acctcggccg cgacgtagtt accaacccga cacaactgct caatctctat acgaataatg
     1261 ccgtcgatct gactgagatg ctggccatgc tcgacgcgca ggcgttcgat accgtcgtct
     1321 tgcgcgctca gttctatccg tcgcccgtgc tcgacgtgat cggccagcgc tacgagacga
     1381 ctgacctcgt gcagatgaac ggtttcgtct actgtatcat gcgcccacga tgaaccagta
     1441 ccgcgtcgaa ctgcccgtct tcaacggccc ccttgatctg ctgctacacc tcatcgagcg
     1501 tgaggaactg gacatcaccg ccgtctccct agtgcaggtg accggccagt atctggagca
     1561 ggtgcggcaa ctgggcgacg agcagctgga gggtctgatc gacttcatca gcatcggcgc
     1621 gcggctcttg ctgatcaaga gccgcgccct gttgccacgt ccgcccgttc tgcccggtga
     1681 cgaagtggaa gaggaagacc cgaccgaggc gctgctgcgg cagttgcggg cctacaaacg
     1741 cttcaagacg gccgctcaat ggctggatgg gcgacagcgg cgcggcctgc gcacctacct
     1801 gcgtgtcgcc ccgccgccac agctggaggg ccatctagac ctcacgggcg tggacgtcaa
     1861 cacgctgttg ggcgcgctcc aggccgtgct ggcgcggacg gaagtgcgcg agagcgctct
     1921 cagtgtcgcc cagccacgga cgctgaccat cgaggatcag ttgcggcggc tgcgcgcccg
     1981 gctggggcgt gggcgggcgt ttctgttcaa cgatgtgctg gtcaacccgc gcgaccggac
     2041 ggaaatcgcc gtgacgctcc tggcactact ggaactgatc aaaaggcgcg aggcccaggc
     2101 gtcgcagagc tatctctttg ggcctatcga ggtgaccgtg gctgggccag aggccgacgc
     2161 gccggccgaa tagctgaacg gcactaacgc gcgtcgcgcc gtcgccgata ggtgacgacc
     2221 gcggccacca caaccagcag caccgccggc gcgcccagac gcaagaacac ccccccgaac
     2281 cgcgccagcg ccagcaccac caccacggcc aggacaatga tcatccagcg cggcagcatg
     2341 cccaatgttt cgaccgcctt cgcgcggcga cgctgggcgg cgcggcaggc gggcgtcgtg
     2401 cccacgcgca gcggatggcg cgccaacgtc cactggcagc ggcactgctc gcactggaga
     2461 caggtgtggt cgcgcaggcc agtttcgtgg tcggtgcgcc gcaggatgcg cccgccacaa
     2521 tggtcgcagt cgtagccggc gtcagcccac tctgtgctga tgtaaatatc cgggttagga
     2581 gcgtgcttgg ccattggttg gggcaaggcc cggggcggcg tagcgctcat ctgggtgata
     2641 ggccggcagc cagtcgccgt gggcggcaag aaggtcatcc accagggcat agatttcgtc
     2701 cggggccagt tcggcggcgg tgtgggggtc gagcagcgcg gcgtggtaga tgtgctcgc
//
LOCUS       DAOSMQ010000434         2755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36739_length_2755_cov_3.741111, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000434 DAOSMQ010000000
VERSION     DAOSMQ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36739_length_2755_cov_3.741111"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..671)
                     /locus_tag="PK829_12405"
     CDS             complement(102..671)
                     /locus_tag="PK829_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOU42058.1"
                     /translation="MRKLIYMMNTSLDGYVEDANGSLDWSSPDEEIHQFFNDQERKFE
                     VSLYGRRLYETMQPYWSTAEQNPDLPAVEKEYARIWNAIPMIVFSRTLDQVEGNATLM
                     RDGLIEQITKLKSQPGRWMSVGGPTLAASLIEAGMVDEFRLVIHPVILGGGKPYFPNL
                     KAPTNLRLLETHHFSSGALYLRYARVSSV"
     gene            complement(720..1373)
                     /locus_tag="PK829_12410"
     CDS             complement(720..1373)
                     /locus_tag="PK829_12410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOU42059.1"
                     /translation="MTQPDGFLAEPASGDPAGAVLVLHAWWGLNDAIRDLCRRLAGAG
                     YVAFAPDVYHGRLATTIAEAEALRGVFGTPEATAQAAADIAAAAHWLYERAGRPAAGL
                     AVIGFSLGAYFALECSTALPDLISKVVVFYGTGPTEFDAARAAFLGHFAANDPYEERE
                     YIDATRAALEAAGRPVTFYDYPDTGHWFFEPDRPEYDAPAAALAWERTLAFLAATQE"
     gene            complement(1486..>2755)
                     /locus_tag="PK829_12415"
     CDS             complement(1486..>2755)
                     /locus_tag="PK829_12415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU42060.1"
                     /translation="LCPQPIDTREQVFYHREMETLIKLQEIAVHMDLEPAEEVGRLPA
                     APTTPPDAPLPCGLPAAPQRPSDRRAAKMNDLGVFHAALPNGGTIPLLKTLLTSACER
                     NCTYCPFRAGRNYRRTTFRPDEMATAFMDMRRAGMVEGLFLSSGIIGGGVRAQDKLLD
                     TIDILRHKHHFGGYVHLKIMPGAEHAQVVRAMQLADRVSVNLEGPNPARLSVLAPKKQ
                     FVEELLTPLRWIEAIRRERPPREGWRGRWPSSVTQFVVGGAGESDLELLHATAYLTRE
                     AGLRRAYFSAFRPVSDTPLEHLPAENPWRQHRLYQASFLFRDYGFDLEEMPFDAGGRL
                     PLDTDPKRAWAEVHLREAPVEVNRAEREELLRVPGIGPKGADAILAARRRARLRSVGD
                     LRQIGVRTKDIEPFVLLDGHRPDYQLRLFT"
BASE COUNT          434 a          871 c          997 g          453 t
ORIGIN      
        1 tcgctcgccg ggcaaggact gtcaaacata actgtggcgc gattctctga attgcaactg
       61 cgattcagag aatcgcgcca cgggcagcaa caggcgcacg cctaaacaga gctgacgcgc
      121 gcgtagcgca gatacagcgc tccgctggaa aagtgatggg tttccaacag ccgcaggttg
      181 gtaggagcct tgaggttcgg gaagtagggc ttgccgccgc cgaggatgac tggatggatc
      241 acgaggcgga actcatcgac catgcccgcc tcgatcagac tggcggcgag ggttgggcca
      301 cccacgctca tccacctgcc cggctgtgat ttcagtttgg tgatctgttc aatcaggcca
      361 tcgcgcatca gggtagcgtt cccttcgact tgatcgaggg tgcgggagaa gacgatcatc
      421 gggatcgcgt tccagatgcg cgcgtattcc ttctccacag cgggcaagtc ggggttctgc
      481 tcggccgtag accaataggg ctgcatggtc tcgtacaggc gccgcccgta caggctaacc
      541 tcaaacttcc gctcctgatc gttgaagaac tggtgaatct cctcgtcggg gctcgaccag
      601 tcaaggctac cattggcatc ttccacgtag ccatccagcg acgtgttcat catgtagatc
      661 agcttgcgca aggttatctc ctttagcggg tctcgaaccc gtggtagcag caggttgttc
      721 tactcctgcg tggcggccag gaaggccagc gtgcgctccc aggccagggc cgcggccggg
      781 gcgtcatact ccggccggtc gggttcaaag aaccagtggc cggtgtcggg gtagtcgtag
      841 aaggtcaccg ggcggccggc ggcctccagc gcggcccgcg tggcgtcgat gtactcgcgc
      901 tcctcgtagg ggtcgttggc ggcgaagtgg cccaggaagg cggcgcgggc ggcgtcgaac
      961 tcggtcggcc ccgtgccgta gaagacgacg actttgctga tgaggtcggg cagcgccgtc
     1021 gaacattcca gggcgaaata ggcccccagc gagaagccga tgacggccag accggccgcc
     1081 ggccgcccgg cgcgctcata gagccagtgc gcggcggcgg cgatatcggc tgcggcctgc
     1141 gccgtggctt cgggcgtgcc gaagacaccg cgcaacgcct cggcttcggc gatggtagtg
     1201 gcgagccggc cgtggtagac gtccggggcg aaggcgacgt agccggcgcc ggccaggcgg
     1261 cggcagaggt cgcggatggc gtcgttcaag ccccaccagg cgtggagcac cagcaccgcg
     1321 ccggccgggt cgccgctcgc gggttccgcc agaaagccat cgggttgggt catcgtcata
     1381 ttactctcct gggttggacg cggccgaacc ccggccgcgc ccctatgggg ttctcggcgc
     1441 cggtgctcgc cggccgccgt cagtcaatgt cccgggccaa tggcctcagg tgaacagccg
     1501 caactggtag tccggccggt ggccgtcgag cagcacaaat ggctcgatgt ctttggtgcg
     1561 cacgccgatc tggcgcaggt cgccgacgga gcgcaggcgg gcgcggcggc gggcggccag
     1621 gatggcgtcg gcccccttcg gcccgatgcc gggcacgcgc agcagttcct cgcgctcggc
     1681 gcggttgacc tccaccggag cttcgcgcag atgcacctcg gcccaggcgc gcttggggtc
     1741 ggtgtccagt ggcaggcggc cgccagcgtc gaagggcatc tcctccagat cgaagccata
     1801 gtcgcggaag aggaaagagg cctgatagag ccggtgctgc cgccaggggt tctcggccgg
     1861 caggtgctcc agcggcgtgt cgctgacggg gcggaaggcg gagaagtagg cccgccgcag
     1921 cccggcctcg cgcgtcaggt acgcggtggc gtgaagtagc tccaggtcgc tctcgccggc
     1981 cccgccgacg acgaactgcg tcaccgacga cggccagcgc ccgcgccagc cctcgcgcgg
     2041 cggccgttcg cggcggatgg cctcgatcca gcgcagcggc gtcagcagct cctcgacgaa
     2101 ctgcttcttg ggggccagga cgctcagccg ggcggggttc ggcccctcca ggttgaccga
     2161 gacgcggtcg gccagctgca tggcgcggac gacctgggca tgctcggcgc cgggcatgat
     2221 cttcaggtgg acgtagccgc cgaaatggtg cttgtggcgc aaaatatcga tggtatcgag
     2281 cagcttgtcc tgggcgcgca cgccgccgcc gatgatgccg ctgctgagga acagcccttc
     2341 gaccatgccg gcgcggcgca tgtccatgaa ggccgtggcc atctcgtcgg ggcggaaggt
     2401 ggtgcggcgg tagttgcggc cggcgcggaa ggggcagtag gtgcagttgc gctcgcaggc
     2461 gctggtcagc agcgtcttga gcagggggat cgtgccgcca ttgggcaggg cggcgtggaa
     2521 gacgcccagg tcgttcatct tggccgcgcg gcgatcgctg gggcgctgcg gcgcggccgg
     2581 caggccacag ggcagcggcg cgtcgggcgg ggtcgttggc gcggccggca gccgcccgac
     2641 ctcctcggcc ggctccagat ccatgtggac ggcgatctct tgcagcttga tgagcgtctc
     2701 catctctcta tgatagaaca cctgttctct ggtgtcaatg ggctgtggac acagg
//
LOCUS       DAOSMQ010000435         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36790_length_2752_cov_4.222469, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000435 DAOSMQ010000000
VERSION     DAOSMQ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36790_length_2752_cov_4.222469"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PK829_12420"
     CDS             <1..187
                     /locus_tag="PK829_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42061.1"
                     /translation="AYGRPELFAARTETVMGRLLATLPEWLAAALPDDGGAILPNRAL
                     VLARSAVRENRATEGEE"
     gene            184..651
                     /locus_tag="PK829_12425"
     CDS             184..651
                     /locus_tag="PK829_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOU42062.1"
                     /translation="MSLETQIAWVGRLMLAALLGIVLGWEREARGRDAGVRTFGAVSL
                     GACLFSIISFAVAPEGRETTRIAAQIVTGVGFLGAGVILRGRGHISGLTTSATLWASA
                     AVGMAVGYGLYLLGAAATALLLFLLLLRRHPRSDMAAGAAAREETENEAAPPI"
     gene            complement(669..1931)
                     /locus_tag="PK829_12430"
     CDS             complement(669..1931)
                     /locus_tag="PK829_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU42063.1"
                     /translation="MADLKIPPRRSRLAPLLGALAVLLLCVTFFLGGVAVDRFLLGGN
                     EASAEETGLNTALLDEARQVIQDNFVDREAATDERLQTGALAGMVDILGDTGHSRFMT
                     PQMVEEQNNYIAGEFEGIGAYVEMRDGFVTIVSPIDNSPAQAAGIQPGDVVTAVDGRD
                     VTGLSLQEVVDLILGPAGTDVTLTLFRPETTEELTLTLTRARIELQNVTWTILPGTTV
                     AQIRIAGFSDHVGDDLAAAITAAEGQGATGLILDLRNNPGGLLSEAINVAGRFLPRDS
                     VVVLRQDANGDVTAEKSDVDDPTTLPLVVLVNQGSASASEIVSGALQDHQRATLVGET
                     TFGTGTVLNEFGLSDGSAILLATEQWLTPNSRVIWRQGIVPDEVVELTGPVQLLVPET
                     ANDLTAEEVAASGDTQVLRAIELLSTPQ"
     gene            complement(1941..2222)
                     /locus_tag="PK829_12435"
     CDS             complement(1941..2222)
                     /locus_tag="PK829_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOU42064.1"
                     /translation="MVSAVVLLKTQRGLINEVATQLADLEGISEVFSVGGRYDLVAIL
                     RVRDNDAMAALVTEHMLKVNGIAESETLLAFRAFSRHDLESMFSLGLEE"
     gene            2369..>2752
                     /locus_tag="PK829_12440"
     CDS             2369..>2752
                     /locus_tag="PK829_12440"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOU42065.1"
                     /translation="MNINPQIQMAFGLRLLIAALLGGVIGLERERRGQDAGIGTFAIV
                     ALGACAFSLGAIITGNADTMRVVAGVIQGIGFLGAGLLIRGRAGVHGLTTAAGLWASA
                     SIGLLASFGLYVLALITTTLILLILI"
BASE COUNT          419 a          929 c          919 g          485 t
ORIGIN      
        1 ggcctatggc cggccggagc tattcgcggc gcgcaccgag acagtcatgg ggcggctgct
       61 ggcaacgctg ccggagtggc tggcggcggc gctgcccgat gacggtggcg cgattctccc
      121 gaatcgcgct cttgtgctgg cgcggagcgc ggttcgggag aatcgcgcca cggagggaga
      181 agagtgagcc tggagacaca aatcgcctgg gttgggcggc tcatgctggc cgccttgctg
      241 ggcatcgtcc tcggttggga gcgcgaggcc cgcggacgcg acgctggcgt gcgcacgttc
      301 ggcgcggtgt cgctgggggc ctgcctcttc tcgatcatct ctttcgccgt agcccccgag
      361 ggacgcgaaa ccacccgcat tgccgcgcag atcgtcaccg gcgtcggctt cctgggcgcg
      421 ggtgtcatcc tgcgcggccg gggccacatt agtggtctga cgacctcggc caccctgtgg
      481 gcctcggccg ccgtgggcat ggccgtcggc tacggcctat acctgctggg cgcggcggcc
      541 acggcgctgc tgctctttct actgctgctg cgccgccacc cacgtagcga catggctgcc
      601 ggcgctgctg cccgcgagga aacggagaac gaggccgcgc cgcccatctg agccgcgccg
      661 cccctctttc actgtggggt gctcagcagt tcgatcgccc gcaacacctg cgtgtcgcca
      721 ctggcggcga cttcctcggc ggtgaggtcg ttggccgtct ccggaaccag gagttgcacc
      781 ggcccggtca gctctaccac ttcgtcgggc acaatcccct gccgccagat gacgcggctg
      841 ttgggcgtca gccactgctc tgtggccagc aggatagccg agccgtccga caggccaaac
      901 tcgttcagca ccgtgccggt gccgaaggtc gtctcgccga ccagcgtcgc ccgctggtgg
      961 tcttgcagcg cgccagagac gatctcgctg gcgctggcgc tgccctgatt gacgagcacc
     1021 accagcggca gcgtcgtcgg gtcgtccacg tcggacttct ccgcggtcac gtcaccgttg
     1081 gcgtcctggc gcaagacgac gaccgaatcc ctgggcagga agcggccggc gacgttgatc
     1141 gcctcactca gcaggccgcc ggggttgttg cgcaggtcga ggatgaggcc ggtggccccc
     1201 tggccctcgg ccgcggtgat ggctgcggcc aggtcatcgc ccacgtggtc gctaaaaccg
     1261 gcgatgcgaa tctgggccac ggtcgtaccc ggcaggatcg tccaggtgac gttctgcaac
     1321 tcgatgcgcg cccgcgtcag cgtcagggtc agttcttcgg tcgtctccgg gcggaagagg
     1381 gtcagcgtca cgtccgtgcc cgccgggccg aggatgagat cgacaacctc ttgcagcgac
     1441 aggccggtca cgtcgcggcc gtcgacggcg gtgacgacgt cgcccggctg gatgccggcg
     1501 gcctgggccg gcgagttatc gatgggcgag acgatggtca cgaagccgtc gcgcatctcc
     1561 acgtaagcgc caatgccctc gaactcaccg gcgatgtagt tgttctgctc ctcaaccatc
     1621 tgcggggtca tgaagcgact gtgccctgtg tcgcccagga tgtcaaccat gcccgccagc
     1681 gcgccggtct gcaagcgctc gtcggtggcg gcctcgcggt cgacaaagtt gtcctggatg
     1741 acctggcggg cctcatcgag aagggcagtg ttcaggccgg tttcctcggc gctggcctcg
     1801 ttgcccccca gcaggaagcg gtcaacggca acgccaccca ggaagaaggt gacgcacaac
     1861 aacaggacgg ccagcgcccc cagcaaggga gccagacggg agcggcgcgg aggtatcttc
     1921 agatcagcca tcgtccgtct ttactcctct agtcccagcg agaacatgct ttccaggtcg
     1981 tgacgcgaga aggcgcgaaa ggccagtaac gtctccgact cggcgatgcc gttgaccttg
     2041 agcatgtgct cggtcaccag cgcggccatg gcgtcgttgt cacgcacgcg caggatggcc
     2101 accaggtcat agcggccgcc caccgaaaag acctcggaga tgccctccag gtcggccaac
     2161 tgggtcgcta cttcattgat cagaccgcgc tgggtcttca gtaacaccac tgcactgacc
     2221 atgccgcttt gctcctcttg ctgttggcgg gtacctttcg cccgcggccg aattgtatca
     2281 cgcccccacc ccggccggtc acattcccag gcgcagacac aacaaaacgc tcatgaaacg
     2341 gccgccgcca gccgctatca tcattcgcat gaatatcaat ccccagattc aaatggcgtt
     2401 cgggctgcgt ctgctgatag ccgcgctgct gggcggggtc atcggcctgg agcgggagcg
     2461 gcgcggccaa gacgcgggca tcggcacgtt cgccattgtc gccctgggcg cgtgcgcctt
     2521 cagcctgggc gctatcatca ccggcaacgc cgataccatg cgcgtcgtcg ccggtgtcat
     2581 ccaggggatt ggctttttgg gcgcggggct gctcattcgc gggcgggccg gcgtccacgg
     2641 actgaccaca gcggccgggc tgtgggccag cgcgtccatt ggcctgctgg ccagcttcgg
     2701 cctctatgtg ctggcgctga tcaccacgac gctgatcttg ctgatcctta tt
//
LOCUS       DAOSMQ010000436         2746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_36899_length_2746_cov_3.745819, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000436 DAOSMQ010000000
VERSION     DAOSMQ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36899_length_2746_cov_3.745819"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="PK829_12445"
     CDS             <1..1501
                     /locus_tag="PK829_12445"
                     /inference="COORDINATES: protein motif:HMM:NF011927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOU42066.1"
                     /translation="MNDREIQAIIDRVMREVGPGGPDAVPASNGAAPAAPPPRPATRP
                     DDRQPTPPAAAPTGDAGVYPTLDEAAAAARAAFVRLNALPLDIRRDMVAHMRQAGREN
                     ALLLAELAAGETGMGRVEDKVLKNVLNADKAPGTEMLDPRAWSGDHGLTLVEYAPYGV
                     IGALTPSTNPTSTVICNAISMIAAGNAVVFNAHPSAKGASNRTVQILNRAIQAAGGPP
                     DLLTCVAEPTLESAQALMQHKLIRLLVVTGGTPVVRAAMQSGKRAVCAGPGNPPVVVD
                     ETADIAQAGRDIVIGGSFDNNIVCTTEKETFAVAAICDDLIRVMEAHGAVRLNSWQFN
                     RLWKAVTTLDRGPRKHADMNKAFIGKNAAVLLAEIGISAGPEVRQIVVEVDADHPAVW
                     SEQMMPIMPVVRVANADEGIDLAVEAEHGFRHTAVMHSKNLDNLSRMAREMDCSIFVK
                     NGPCLAGLGYGGEGFCSFTIASPTGEGITNPISFSRIRRCALVDSFRIV"
     gene            1507..2094
                     /locus_tag="PK829_12450"
     CDS             1507..2094
                     /locus_tag="PK829_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOU42067.1"
                     /translation="MTAPALALLEFSSLAAGIEAGDAMVKRAPVEQLLTGTIQPGYYL
                     ALVIGEVADVNEAVAAGLLASGAYLRDQLLLPNVHPGVVAALRGARATGTAEALGVIE
                     TTTVAAAIRAADAGLKGAEVALNQLRLGDGLGGKGLALFVGLVSDVEAAVAIGSDAVG
                     VQLLRRAVIARLHGEMWANVDGSGRFGDHFGWPAT"
     gene            2100..2378
                     /locus_tag="PK829_12455"
     CDS             2100..2378
                     /locus_tag="PK829_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOU42068.1"
                     /translation="MQLARVIGTVVATRKYAGLEGIKFLVVQPLSKTQQPAGAPVVAA
                     DATAQAAPGELVFIVGSREAAQAMPEPFVPVDHAIVGLVDDVALDDNP"
     gene            2383..2661
                     /locus_tag="PK829_12460"
     CDS             2383..2661
                     /locus_tag="PK829_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOU42069.1"
                     /translation="MYIGRVAGTVVATVKHEAFHGHKLLLVDRLDLAGQPTAEYDICV
                     DMARAGVGDRVLVLDEGNGARQVLNRQVAPVRAVIVGIVDDVYLMDGH"
BASE COUNT          426 a         1018 c          922 g          380 t
ORIGIN      
        1 catgaatgac cgcgagattc aggccatcat cgaccgcgtc atgcgggagg ttgggccagg
       61 aggccccgac gctgtgcccg ccagcaacgg cgccgcgccg gccgcgccgc ccccgcgccc
      121 agccacccgc cccgacgacc gccagcccac gcccccggcg gccgcgccca caggcgacgc
      181 cggcgtctac cccacgctcg acgaggccgc cgcggccgcc cgcgccgcct tcgtgcgcct
      241 caacgccctg ccgctcgaca tccgccgtga catggtcgcc cacatgcgcc aggccggccg
      301 ggagaacgcc ctgctgctgg ccgagctggc cgccggcgag acgggcatgg gccgcgtcga
      361 ggacaaggtg ctcaagaacg tcctcaacgc cgacaaggcc cccggcacgg agatgctcga
      421 cccgcgcgcc tggagcggcg accacggcct gacgctggtc gaatacgccc cctatggcgt
      481 catcggcgcg ctgaccccca gcaccaaccc gaccagcacg gtcatctgca acgccatcag
      541 catgatcgcc gccggcaacg ccgtcgtctt caatgcccac cccagcgcca agggggcgtc
      601 caaccgcacg gtgcagatcc tcaatcgggc catccaggcc gccggcggcc cgcccgacct
      661 gctcacctgc gtggccgagc cgacgctgga atcggcccag gcgctgatgc agcacaagct
      721 catccgcctg ctggtcgtca ccgggggcac gccggtcgtg cgcgcggcca tgcagagcgg
      781 caagcgcgcc gtctgcgccg ggccgggcaa cccgcccgtg gtcgtcgatg agacggccga
      841 catcgcccag gccggccgcg acatcgtcat cggcggctcg ttcgacaaca acatcgtctg
      901 cacgacggag aaagaaacct tcgccgtggc cgccatctgc gacgacctca ttcgcgtcat
      961 ggaggcccac ggcgcggtgc ggctcaactc gtggcagttc aaccgcctgt ggaaggcggt
     1021 cacgacgctc gaccgcggcc cgcgcaagca cgccgacatg aacaaggcct tcatcggcaa
     1081 gaacgccgcc gtgctgctgg ccgagatcgg catcagcgcc gggccggaag tgcgccagat
     1141 cgtcgtcgag gtggacgccg accaccccgc cgtctggtcg gagcagatga tgcccatcat
     1201 gccggtcgtg cgcgtggcca acgccgacga gggcatcgac ctggccgtcg aggccgagca
     1261 cggtttccgc cacacggcgg tcatgcactc caagaacctc gacaacctca gccgcatggc
     1321 acgggagatg gattgcagca tctttgtgaa gaatggcccc tgcctggcgg gcctgggcta
     1381 tggcggcgag ggcttctgct cgttcaccat cgccagcccc accggcgagg gcatcaccaa
     1441 cccgatcagc ttcagccgca tccgccgctg cgccctggtc gattcgttcc gcatcgtctg
     1501 acgactatga ctgcgcccgc cctcgccctg cttgagttca gtagcctcgc cgccggcatc
     1561 gaggcgggcg acgccatggt caagcgcgcc ccggtggagc aactgctgac cgggacgatc
     1621 cagccaggct actacctggc cctggtcatc ggcgaggtgg ccgacgtcaa cgaggccgtc
     1681 gcggccgggc tgctggcgag tggggcctat ctgcgcgacc aactgctgct gcccaacgtc
     1741 caccccggcg tcgtggccgc cctgcgcggg gcgcgcgcca cgggcacggc cgaggcgctg
     1801 ggcgtcattg agacgaccac cgtggccgcg gccatccgcg ccgccgacgc cgggctgaag
     1861 ggggccgagg tggccctcaa ccaactccgg ctgggcgacg ggctgggcgg caaggggctg
     1921 gcgctgttcg tcgggctggt gtcggacgtg gaggcggccg tggccattgg cagcgacgcc
     1981 gtcggcgttc aactgttgcg ccgcgccgtc atcgcccggc tgcacggcga gatgtgggcc
     2041 aacgtcgatg gcagcggccg gttcggcgac catttcggct ggccggccac ctgaacacca
     2101 tgcaactcgc gcgcgtcatc ggcacggtcg tagcgacccg caagtatgcg gggctggagg
     2161 gcatcaagtt cctcgtcgtc cagccgctca gcaagacgca gcagccggcc ggcgcgcccg
     2221 tcgtcgccgc cgacgccacg gcccaggccg cgccgggcga actggtcttc atcgtcggca
     2281 gccgcgaggc cgcccaggcc atgccggagc cgttcgtgcc cgtcgatcac gccatcgtcg
     2341 gcctcgtcga cgacgtggcc ctggacgaca acccctagcc ccatgtatat cggacgcgtc
     2401 gccggaaccg tggtcgccac ggtcaaacat gaagccttcc acggccacaa gctgctgctg
     2461 gttgaccggc tcgatctggc ggggcagccc acggccgagt acgacatctg cgtcgatatg
     2521 gcccgcgccg gcgtgggcga ccgcgtgctg gtgctggacg agggcaacgg cgcgcgccag
     2581 gtgctcaatc gccaggtcgc gccggtgcgg gcggtgattg tggggattgt tgacgacgtc
     2641 tatctgatgg atggccacta agccggcggc cggcggctac tttatcagca ctcacccttt
     2701 ttctggacag ccgccatttg cccggccagg cggtaggcag tctggt
//
LOCUS       DAOSMQ010000437         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37083_length_2736_cov_4.016785, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000437 DAOSMQ010000000
VERSION     DAOSMQ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37083_length_2736_cov_4.016785"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1547
                     /gene="murC"
                     /locus_tag="PK829_12465"
     CDS             114..1547
                     /gene="murC"
                     /locus_tag="PK829_12465"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU42070.1"
                     /translation="MHIHVVGIGGAGMSAIARVLLGRGYRVSGSDRQANSQTAALAAE
                     GIRVSIGHDPANIDGADLLLVSSAVPESNEEWVAAQERGLPVLKRADLLGQLMRGSIG
                     IAVAGTHGKTTTTGMIAHILLEAGLDPTVILGGTLDELGGNGRFGTGPHFVIEADEYD
                     YMFLGLRPEVAVITNVEHDHPDLFPTAETYRAAFRQFVEGLPETGRLIVCADDPGAVA
                     LLDELTIVPGATASIDVITYGLGSLPRSETPTVRALDPRPNRIGGHDFIVDVDGQTIG
                     LARLRLPGLHNVRNSLAAIIVSLGLQLDFASICRALAGFGGVQRRFQLIGEVGDVTII
                     DDYAHHPTEIRATLAAARQRFPGRRLWAVWQPHTFSRTRLLADEFAGSFDAADRVIAL
                     DIYRSREAGDPSISTAGVVSRMSHPQAVHIPDRRAAADYLLERVSPGDVILTLGAGDG
                     NMVGRWLLEDLQRQRGVPSRAAGQPGL"
     gene            1553..2494
                     /gene="murB"
                     /locus_tag="PK829_12470"
     CDS             1553..2494
                     /gene="murB"
                     /locus_tag="PK829_12470"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOU42071.1"
                     /translation="MTTLHTVERLRALFGDRLAISEPLARHTTTRVGGPAELFLSVTS
                     AGELLAAVEVAYAAGLPYFILGGGSNILVADAGISGLVILNRARQVSFRHSGAGVVCT
                     AESGANLSALARQCISRGLGGLEWAIGVPGTVGGAVVGNAGAHGADMDATLLATTIWE
                     PGYGTRVYNREEMAYGYRTSVLKRDMAPGRARRVVLSAELRLTPAPVEVLTARADGFT
                     AHRKERQPGGATTGSMFKNPENFYAGYLIDTAGLKGLRIGDAIISDKHANFFVNDGQA
                     TAEDIRALIAEAWNSVRDQFGIEMELEVELVGDWAFE"
     gene            2595..>2736
                     /locus_tag="PK829_12475"
     CDS             2595..>2736
                     /locus_tag="PK829_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HOU42072.1"
                     /translation="MTTGKLRIGVLFGGRSGEHEVSLRSARSVMAALDPDRYEVVPIG
                     ITK"
BASE COUNT          444 a          987 c          853 g          452 t
ORIGIN      
        1 tgacggcatc gccctacttc ctgccgctgc tactggtggt ggtgtttctg ttcatcctgg
       61 tggtgctgct gtaaatgagt aatggcatga cgagcgcgga actcagaccc ggaatgcata
      121 ttcacgtcgt cggcatcggt ggcgccggca tgtcggccat cgcccgcgtg ctgctcggcc
      181 gtggctaccg ggtcagcggc tccgaccggc aggccaatag ccagacagcc gccctggccg
      241 ccgagggcat ccgtgtgtcc atcggccacg atccggccaa catcgacggc gcggacctgc
      301 ttctcgtctc ttcggccgtg ccggagtcga atgaggagtg ggttgccgcc caggagcgcg
      361 gcctacccgt cctcaaacgc gccgacctgc tcggccagct catgcgcggc tccatcggca
      421 tcgccgtggc cggcacccac ggcaaaacga ccaccacggg catgatcgcc cacatcctcc
      481 tggaggccgg cctcgaccca accgtgatcc tgggcggcac gctggacgaa ctgggcggca
      541 atggccgctt tggcactggg ccgcacttcg ttatcgaggc cgatgagtac gactacatgt
      601 tcctcggcct gcgcccggaa gtcgccgtca tcaccaacgt cgagcacgac catcccgacc
      661 tgttccccac ggccgagacc tatcgcgccg ccttccgcca gttcgtcgag ggattgccgg
      721 agaccggccg tctcattgtc tgcgccgacg accccggcgc ggtggccctg ctggacgaac
      781 tgactatcgt ccccggggcc acggccagca tcgacgtgat cacctatggc ctgggcagcc
      841 tgccccgctc ggagacgccc accgtgcgcg cccttgaccc ccgccccaac cgcatcggcg
      901 gccatgactt catcgtcgat gtcgacggcc agaccatcgg cctggcccgg ctgcgcctgc
      961 ccggcctcca caatgtccgc aacagcctgg cggccatcat cgtcagcctg ggattgcaac
     1021 tcgacttcgc cagcatttgc cgcgccctgg ccggcttcgg cggagtgcag cgccgcttcc
     1081 agctcattgg cgaagtgggc gatgtaacca tcatcgatga ctacgcccac caccccaccg
     1141 agatacgggc cacgttggcc gccgcccgcc agcgcttccc cggccggcgc ttgtgggccg
     1201 tctggcagcc ccacaccttc agccgcacgc gcctgctggc cgacgagttc gccggcagct
     1261 tcgacgccgc cgaccgcgta attgccctgg atatctaccg cagccgcgag gccggcgacc
     1321 cgtctatcag caccgccggc gtcgtcagcc gcatgtccca tccgcaggcc gtgcatattc
     1381 ccgaccggcg cgcggctgcc gattacctac tggagcgagt tagccccggc gacgtgattc
     1441 tgaccctcgg cgctggcgat ggcaacatgg tcggacgctg gcttttagaa gatctacaac
     1501 ggcaaagagg agtgccgagc cgggccgccg gccaaccggg cctatgaaac ttatgaccac
     1561 gctccacacg gtagagagac tgcgcgccct ttttggcgac cgcctggcca tcagcgaacc
     1621 gttggcgcgg cacacgacca cccgcgtcgg cgggccggcc gagctgtttc tgtcggtcac
     1681 gtcggccggc gaactgctgg ccgcggtcga ggtcgcctac gccgccggcc tgccctattt
     1741 catcctgggc ggcggctcca acatcctcgt cgccgacgct ggcatcagcg gcctggtcat
     1801 cctcaaccgg gcgcgccagg tcagcttccg ccacagcggc gcgggcgtcg tctgcacggc
     1861 cgagtcgggc gccaacctgt ccgccctggc tcgccagtgc atcagccggg gcctgggcgg
     1921 gctggagtgg gccatcggcg tgccgggcac tgtcggcggc gcggtggtcg gcaacgccgg
     1981 cgcccacggc gcggacatgg atgccacgct gttggcaacg accatctggg agccgggcta
     2041 tggcacgcgc gtctacaacc gcgaagagat ggcctacggc tatcgcacca gcgtcctgaa
     2101 acgcgacatg gcccccggcc gggcgcgccg tgtcgtcctg tcggccgagt tgcgcctgac
     2161 gccggctccg gtcgaggtgc tgaccgcccg cgccgatggc ttcaccgccc accgcaagga
     2221 gcgccagccc ggcggagcca ccaccggctc catgttcaag aacccggaga acttctacgc
     2281 cggctatctg atcgataccg ccggcttgaa gggcctgcgc atcggcgacg cgatcatttc
     2341 ggacaaacac gccaacttct tcgtcaacga cggccaggcg acggccgagg acatccgcgc
     2401 tctcatcgcc gaggcctgga acagcgtccg tgaccagttc ggcatcgaga tggaactcga
     2461 ggtcgagctg gtgggcgact gggcgtttga atgaattagg aattaggaac taagaccgat
     2521 aagaggcaac tgactgactg acctactgac tgactgatta ctggacactg attactggac
     2581 acagactacc gctcatgacc actggcaaac tccgcattgg cgtcctcttc ggcggccgct
     2641 ccggcgagca tgaagtctcg ctgcgttcgg cccgctcggt gatggccgcg ctcgaccccg
     2701 accgctacga ggtcgtgccc atcggcatca ccaagg
//
LOCUS       DAOSMQ010000438         2721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37347_length_2721_cov_3.011253, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000438 DAOSMQ010000000
VERSION     DAOSMQ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37347_length_2721_cov_3.011253"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2687)
                     /locus_tag="PK829_12480"
     CDS             complement(<1..2687)
                     /locus_tag="PK829_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU42073.1"
                     /translation="MSTPTNIWGAAIPGLSLSGPLAFSLALLLASLVLLVLYRLRAAA
                     VPGTMTRQQWLVLGALCLASLALSQLLPLRLPWADPLLREHPVTAFLALFAAVPALLA
                     GAALNIPAAIVVGLFAGLGRALGQTGSPADILAAGLAAGASAWLMQQNYSGRLFRALR
                     QPVVAGLLGWLLWGIITVLDILASMWPQMGLLAALDLALFLGAYALIPLTLEGLTGGL
                     LAMLLLWIAPQWRPARGLVPSPLRRSLQRQLTAAFLSFVAVVLLVSALLVFLLSARFA
                     RQAIAGQMAANTDAVAVRLGTLQRDLATALAAGGADPALAAADPGARSAALSRLLRAA
                     PQFSQVDAIAAPADLASLTPAESALVADALASGEARFGLLEAAGERHIGLALPHPGSA
                     SDGVLLGHVSTAALEAVVANLSPTDGASGLVVDEGGRVVLAAGPAQPATEWAAPPAEQ
                     AALALGGGRVVYRARAGDGARQLVYYVPVPPSGWRVVAVVPQARILRAALGVMGPLAL
                     LLAAVSGLFVAYVAGLGRAIVRPLGEISQASRAIAGGGGLERPVRSRREDEIGQLSLA
                     FSQMQRALRQRLDELSLLLGVSNDVAATVNLGEGMVAVLQGVLRGTGAAGARAVIRNP
                     NGPAPLVFAEGPAAESMAPLDRAVVRRMRAEVELGLTAPEELDTELGAAPVAALFALP
                     LRLAGEYQGALYLGYRQPHYFDSDERNLLRTLAGQASVLVQNAYLFAAAEGGRRRLAA
                     VLASTPNAVIVTDQTDRVLLLNPAMERVFGLRAADAIGRPVVDVLAGSETGRTLAQQL
                     TAGQDVAADNRLDLAAGGRDFLAGVSIVSTSDGQAIGRVAVLQDVTDLKEVDRLKSEF
                     LDGISHDLRSPLTYMRNYASLLPLPDDPALEQQYVNKIAA"
BASE COUNT          461 a          960 c          968 g          332 t
ORIGIN      
        1 gcggcgatct tgttgacgta ctgctgctcc agcgccgggt cgtcgggcag cggcaacagg
       61 ctggcgtagt tgcgcatgta ggtcagaggg ctgcgcaggt cgtggctgat gccgtcgaga
      121 aactctgact tcaaccggtc tacctctttc aagtcggtca catcttgcaa gaccgccacg
      181 cggccgatgg cctgcccgtc gctggtagag acgatcgaga cgccggccag gaaatcgcgc
      241 cccccggcgg ccaggtcgag ccggttgtcg gcggcgacat cctggccggc ggtcagctgt
      301 tgggccagcg tccggcccgt ctcgctgccg gccagcacat cgaccacggg acggccaatg
      361 gcgtcggcgg cgcgcagacc gaaaacacgc tccatcgccg ggttcagcag cagcacccgg
      421 tcggtctggt cggtgacgat cacggcgttg ggcgtgctgg ccaaaacggc agccaggcga
      481 cggcggccgc cctcggcggc ggcgaacaga taggcgtttt gcaccagcac cgacgcctgt
      541 ccggccagcg tccgcagcag attgcgctcg tcactgtcga agtagtgggg ctggcgataa
      601 cccaggtaga gcgcgccctg atactcgccc gccagccgca gcggcagggc gaacagcgcc
      661 gccaccgggg ccgcgcccag ttcggtgtca agctcctccg gcgcggtgag gcccagttct
      721 acctcggcgc gcatccgccg gacgacggca cgatcaagcg gagccatgct ctcggccgcc
      781 ggcccctcgg cgaaaaccag cggcgcgggg ccattggggt tgcggatgac cgctcgcgcc
      841 ccggccgcgc ccgtgcctcg caagactcct tgtagcacgg caaccatgcc ttcgcccagg
      901 ttcaccgttg ccgccacgtc gttgctgacg cccagcagca gcgacaactc gtctagccgt
      961 tggcgcaggg cgcgctgcat ctgcgagaag gccagactga gctggccgat ctcgtcttcg
     1021 cgccggctgc gcaccggccg ctccaggccg ccgccgccgg cgatggcccg cgaggcctgg
     1081 ctaatttcgc ccagcggccg gacgatggcc cgccccaacc cggccacata ggcgacgaac
     1141 agcccgctga cggccgccag cagtagcgcc agtggcccca tgacgcccag cgcggcccgc
     1201 agaatccgcg cctggggcac gacagcgacg acgcgccagc cgctcggggg cacagggaca
     1261 tagtagacca gttgtcgcgc gccatcgccg gcgcgcgcgc gatagacgac gcgaccgccg
     1321 cccagcgcca atgcggcctg ctcggccggg ggcgcggccc actccgtcgc cggttgggct
     1381 gggccggcgg cgaggacgac gcgcccgcct tcgtcaacca ccaggccgct cgcgccatct
     1441 gtgggcgaga ggttggccac cactgcctct aaagctgcgg tcgaaacgtg ccccagcagc
     1501 actccgtcgc tggcgctgcc tggatggggc agggccaggc cgatgtgccg ctcgccggcc
     1561 gcctctaata ggccgaagcg cgcttcgccg gaggccagcg catcggccac cagagcgctc
     1621 tcggccggtg tcaggctggc caggtcggcc ggtgcggcga tggcgtctac ctggctaaac
     1681 tggggcgcgg cgcggagcag ccggctgagc gcggcggagc gcgcgccggg gtcggccgcg
     1741 gccagcgccg ggtcggcccc gccagcggcc agcgcggtgg ccaggtcgcg ctgtaacgtg
     1801 cccaggcgga cggcgacggc gtcggtgttg gccgccatct gcccggcgat ggcctggcgg
     1861 gcgaagcggg ccgacagcag gaataccagc agggcgctga ccagcaacac cacggccaca
     1921 aaggagagga aggccgccgt caattgccgc tgcaagctgc ggcggagggg cgacggcacg
     1981 aggccgcgcg ccggccgcca ctggggggca atccatagga gcagcatggc cagtaggccg
     2041 ccggtcagcc cctccagcgt cagcgggatg agggcatatg cccccaggaa cagggccaaa
     2101 tccagcgcgg ccagcaggcc catctgcggc cacatgctgg ccaagatgtc caggacggtg
     2161 atgattcccc agagcagcca gccaagcagc ccggcgacaa ccggctgtcg caatgctcgg
     2221 aagagacggc cggagtagtt ctgctgcatc agccaggccg aggccccggc cgccagaccg
     2281 gcggccagga tgtctgccgg cgaaccggtt tggcccaaag cgcggcccag cccggcgaag
     2341 agaccaacga cgatggccgc ggggatgttg agcgccgcgc cagccagcag agccgggacg
     2401 gcggcaaaga gcgccaaaaa ggccgtcacc gggtgttcgc gcagcagggg atcggcccat
     2461 ggcaggcgca gaggtagcag ctgactgagg gccagcgatg ccagacagag cgcgccaaga
     2521 accagccatt gctgccgggt catggttccc ggaacggccg cggcgcgtag acgatagagg
     2581 acaagcagca ccagactggc cagaaggagc gccaggctaa aggccaacgg cccggacagc
     2641 gacagacccg gtatggcggc tccccagatg ttggtgggcg tactcacgct acggtcactc
     2701 gcctgccact acgccgattc c
//
LOCUS       DAOSMQ010000439         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37510_length_2712_cov_3.764772, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000439 DAOSMQ010000000
VERSION     DAOSMQ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37510_length_2712_cov_3.764772"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK829_12485"
     CDS             complement(<1..607)
                     /locus_tag="PK829_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU42074.1"
                     /translation="MDTWQWIGGFIPVEAVLLANSREMRQIYVTRERLDSRLARLERL
                     ASERRVPVGQSDSDTLTDLAGAPGHSGVAAQVGPRRTVALADLLASPAPALVMLDGVE
                     DPYNFGQAVRSLYAAGLDGLIVRQRHWLSAGVAVRASAGATEFMPTASVETPEEAAAE
                     ARARGLRVAVADEEGTPMAQTDLSGPLLLVIGGEKRGVTRSF"
     gene            complement(711..2093)
                     /locus_tag="PK829_12490"
     CDS             complement(711..2093)
                     /locus_tag="PK829_12490"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOU42075.1"
                     /translation="MAKKPQTPQRDDAQQRQSRKEILIARKHERQLRNVRIAAVVVVV
                     LIAIVVAVALINELALTPKRAVATIGDQSIALRDWQQRVTYERAQRIIFLENQLDAFG
                     GDVGIVQQFGGSVINELLDGEGLGENVLNVMAEELVICNAAAERDLAITDADVQNEIN
                     ANFGFYGEGVSPTAQPEPTQTIQPTPSLTPITPADATAVPTEAPEPTATAGPSPTPFP
                     TPTPLSEQAFQEQFGGIMTSLTDLGASEATYRSVVRAQLCRTRLADALAEEQSLSRTA
                     PQASIFVITADTEEAANEVQTQIEADGYLTAWNTIQSRVDDPEATETPPTSSFELLWR
                     TQDGLEASVGAAVAALAFDLPVNEPSDIIAVDNGDDTTTYYIIMVSGREERELSESEY
                     QTRQDEMLQTFADEQLTGKLQLNELWRSRVPTVPVLDSKFLAAPTATPEPEATEAVVP
                     TIAFEPTAAP"
     gene            complement(2117..2356)
                     /gene="rpsR"
                     /locus_tag="PK829_12495"
     CDS             complement(2117..2356)
                     /gene="rpsR"
                     /locus_tag="PK829_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOU42076.1"
                     /translation="MQANNRPNRRFRPSKRVCQFCVEKTPIDYKQVDVLSRYVNEFGR
                     MRPRRQTGTCAKHQRALAREIKRARHIALLPFVSD"
     gene            complement(2370..2708)
                     /gene="ssb"
                     /locus_tag="PK829_12500"
     CDS             complement(2370..2708)
                     /gene="ssb"
                     /locus_tag="PK829_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU42077.1"
                     /translation="MASNRGLNKVMVIGWLEDDPEVRQTPSGRAVASFSVATSRAWTS
                     SEGDRHEEKEWFNVVAWGTLAEMCKDQVLKGQQIYIEGRLQTRSWEDSLGRKHFRTEV
                     VAQELIVLSD"
BASE COUNT          427 a          882 c          901 g          502 t
ORIGIN      
        1 gaaaggagcg cgtgacgccg cgcttctcgc cgccgatgac caacagcagc ggcccgctca
       61 ggtcggtctg ggccatcggc gtgccctctt cgtcggcaac ggcgacccgc aggccgcggg
      121 cgcgcgcctc ggcggcagcc tcttcgggcg tctccaccga ggcagtgggc atgaactccg
      181 tcgccccggc cgaagcgcgc acagccaccc cggccgacag ccagtggcgt tgccgcacaa
      241 tcagcccgtc gaggccggcg gcatagagcg aacgcacggc ctggccaaag ttgtaggggt
      301 cctccacgcc atcgagcatc accagcgccg gcgccgggct ggccaacaga tcggccaacg
      361 ccactgtccg gcgcggaccc acttgcgcgg cgacaccact gtggccgggc gcgccggcca
      421 gatcggtcaa cgtatcgctg tcgctctggc ccacgggcac gcggcgctcg ctggccagcc
      481 gttccagccg ggccagccgg ctgtcaaggc gctcacgcgt gacgtagatc tgtcgcatct
      541 cgcggctatt ggccagaagc accgcctcca cgggaataaa cccgccaatc cattgccagg
      601 tgtccatgct tttgcgctcc gctagacaaa gaaaaggcca gacaggctgg ccttttcatc
      661 acctcaaatc atgtccccgc cggcggggcg gcttacccgg ctgcgacgag ctacggcgcg
      721 gccgtcggtt cgaaggcaat cgtcggaacc acggcttcgg ttgcttccgg ctccggcgtg
      781 gccgtgggcg cggccaggaa cttgctgtcg agcaccggca ctgtgggcac gcggctgcgc
      841 cacagttcat tgagttgcag cttgccggtc agttgctcat cggcgaacgt ctgcaacatc
      901 tcgtcctggc gcgtctgata ttcgctctcg gacaactcgc gctcttcgcg gccactgacc
      961 atgatgatgt agtaggtggt cgtgtcgtcg ccgttgtcca cggcgatgat gtcactcggc
     1021 tcgttgacgg gcaggtcgaa cgccagcgca gccaccgcgg ccccaacgct ggcctccagg
     1081 ccatcctgcg ttcgccacag cagctcaaac gaggacgtgg ggggcgtctc agtcgcttcg
     1141 gggtcatcga cgcggctctg gatggtgttc caggccgtca gatagccgtc ggcctcgatc
     1201 tgggtctgaa cctcattggc ggcctcctcg gtatcggcgg tgatgacgaa gatgctggcc
     1261 tgcggggccg tgcgcgacag tgactgctct tcggccaggg cgtcggccag gcgcgtgcgg
     1321 cacagttgcg cgcgcacgac actgcggtaa gtcgcctcgc tcgcgcccag gtcagtcagc
     1381 gaggtcatga tcccgccgaa ctgctcctgg aaggcctgtt ccgacagcgg cgtgggcgtc
     1441 gggaagggcg tcggcgaggg gccggccgtg gccgtcggct ccggcgcttc cgtcggcacg
     1501 gcggtggcgt cagccggggt gatgggcgtc agcgacggcg tcggctggat ggtctgcgtc
     1561 ggctccggct gcgcggtggg cgagacgccc tccccgtaga agccgaagtt ggcattgatc
     1621 tcgttctgca cgtcggcgtc ggtaatggcg aggtcgcgct cggcggcggc attacagatg
     1681 accagttctt cggccatcac gttcagcacg ttttcgccca ggccctcgcc gtcgaggagc
     1741 tcattgatga cactgccacc gaactgctgg acaatgccga cgtcgccgcc aaatgcatcc
     1801 aactggtttt ccagaaagat gatgcgttgg gcgcgctcat aggtgacgcg ctgctgccag
     1861 tcgcgcaggg caatggattg gtcgccgatc gtggcgacgg cgcgcttggg cgtcagggcc
     1921 agttcattga tcagagccac cgcaacgacg atggcgatga gaacgacgac gacaacggcg
     1981 gcgatgcgca cgttgcgtaa ctggcgctcg tgcttgcgcg cgatcagaat ctctttgcgc
     2041 gactgacgct gttgggcgtc gtcccgctgg ggggtttggg gtttcttggc catagcgagt
     2101 gtccgccgcg cgaggcctaa tcagaaacga aaggcagcag agcgatgtgt cgggcgcgct
     2161 tgatctcgcg ggcgagagcg cgctggtgtt tggcgcacgt gcccgtctgg cggcgcggcc
     2221 gcatgcggcc gaactcattc acatagcggc tgagcacatc cacttgctta tagtcgatgg
     2281 gcgtcttctc gacgcagaac tggcacaccc gcttgctggg gcgaaaacgc cggttaggac
     2341 ggttattagc ttgcatgtct aatcctcttt caatctgaga gcacaatcag ttcctgggcg
     2401 actacttcgg tgcgaaagtg cttgcggccg agactgtctt cccagctgcg cgtctgtagc
     2461 cgcccctcaa tgtagatctg ctggcccttc aggacctggt ccttgcacat ctcggccagc
     2521 gttccccagg cgacgacatt gaaccactcc ttctcttcgt gccggtcgcc ctcggacgat
     2581 gtccaggcgc gtgaggtggc aacgctgaag gaggccacgg cgcggccgct gggtgtctgt
     2641 cggacttcgg ggtcgtcttc cagccagccg atgaccatga ctttgttcaa gcctctgttc
     2701 gacgccatcg cg
//
LOCUS       DAOSMQ010000440         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37512_length_2712_cov_3.448250, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000440 DAOSMQ010000000
VERSION     DAOSMQ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37512_length_2712_cov_3.448250"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="PK829_12505"
     CDS             complement(<1..1301)
                     /locus_tag="PK829_12505"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOU42078.1"
                     /translation="MTTERPDLSGVSADVVAYIEALEAELAHQRGRVEPPRAAALPDE
                     LPTPYSIITISRRGAAKRSPRHLYSRQRRGGMGVFDIDAADDDEPALLAHAHENDILI
                     LFTGDGRAFRLPATQLPESPVRARGAALAGKLPLRPGERVVGALAEGGGQYAILLSQR
                     GWVRRVRASYLGRSLIQGTSFHDIKEGGPLVAVCWSGGGDDLFLASREGKAIRFMETQ
                     VPTRGVLGIRLDVTDAAVAVTPVATVAVATGVFLLGADGQGTIRLMSGFAANKAPAAA
                     GKVAMKTERLVGAVAAGPGDELFILSRLGKKVVVLGVPQTYPVTPVNGWMISGFLAPD
                     LDADYTYPKALKHELREKTGEYVLDVKDFRTEEKDRLLAQVHRLMNNRFDVARYLMTT
                     RPWDFFMMVEMGLDRLHHGFWKYGDPSHPKYEPGNPYEHAMR"
     gene            complement(1298..1987)
                     /locus_tag="PK829_12510"
     CDS             complement(1298..1987)
                     /locus_tag="PK829_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOU42079.1"
                     /translation="MNAPLDDGDIVSGALASPPAADHLEVEVKFLVADLDVVRRRLAG
                     AGATDGAPRVHERNVRFDTADESLLARQALLRLRQDTRVRLTYKGLAAQDAASEAKIR
                     EELELEVADFDRMALILARLGFGPVQTYEKYRQTYHWGDVEVVLDEMPFGTFVELEGP
                     SDKSLKAAAAALGLDWERRVLANYLALMELARSTFSLPFRDLTFANFERHPVDMGTLL
                     PVCVVVEGGDW"
     gene            complement(1984..2511)
                     /locus_tag="PK829_12515"
     CDS             complement(1984..2511)
                     /locus_tag="PK829_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42080.1"
                     /translation="MKYIATVKGQEYTIEIDPDRGILIDDQPYEIDFRRLPSGGVTSL
                     LMNNRSIAAMVEERGNQWDVLIQGELYNVQVQDERAYRLDRMRTSGLSVDGEAVVASP
                     MPGIIVAVPVAVGDAVEFGQRVIILESMKMENELRAPCAGVVTHVHVAAGTSVEKDQP
                     LLGISQESGGIGSAS"
     gene            complement(2513..>2712)
                     /locus_tag="PK829_12520"
     CDS             complement(2513..>2712)
                     /locus_tag="PK829_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42081.1"
                     /translation="GQFDTKFVEDRFSMDDRGAPHTLEAALLATLAAHEQSQRAAQIV
                     APGARDTSNWKWYSRWERLRR"
BASE COUNT          450 a          929 c          880 g          453 t
ORIGIN      
        1 cgcatggcgt gctcataagg gttgccgggc tcgtacttgg ggtgggaggg gtcgccgtat
       61 ttccaaaacc cgtggtgcag ccggtccagg cccatctcca ccatcatgaa gaagtcccag
      121 ggccgggtgg tcatcaggta gcgcgccacg tcgaaccggt tgttcatgag gcggtggacc
      181 tgcgccagca accggtcctt ctcctcggtc cggaagtcct tcacgtcaag aacatactcg
      241 cccgtttttt cgcgcagctc atgcttgagc gccttggggt acgtgtaatc cgcatccagg
      301 tccggcgcta agaacccaga tatcatccag ccgttgacgg gcgtgaccgg gtacgtctgc
      361 ggcacgccga gcactacgac cttcttgccc agccgcgaga ggatgaatag ctcatcgccg
      421 gggccggcgg ccaccgcgcc gaccagccgt tcggtcttca tggccacctt gccggccgcg
      481 gcgggagcct tgttggccgc gaagccgctc atgaggcgga tcgtcccctg gccgtcggcc
      541 cccagcagaa agacgcccgt ggccacggca accgtggcca cgggggtcac ggccacggcc
      601 gcgtcggtca cgtccagccg gatgcccaga acgccgcgcg tgggcacttg ggtctccatg
      661 aagcggatgg ccttgccctc gcgcgaggcc aggaaaaggt cgtcgccgcc gcccgaccag
      721 cacacagcca ccagcggccc gccctctttg atgtcgtgga agcttgtgcc ctggatgagg
      781 ctgcggccca aatagctggc acgcacgcgc cgcacccaac cgcgctggct caacaggatg
      841 gcgtattgcc cgccgccctc ggccagcgcg ccgacgacac gctcgcctgg ccgcagcggc
      901 agcttgccgg ccagagccgc gccgcgcgcc cgcaccggcg actctggcaa ctgggtggcc
      961 ggcagacgaa aggcgcggcc gtcgccggtg aacaggatca ggatatcgtt ctcgtgggcg
     1021 tgggccagca gcgccggctc gtcgtcatca gccgcgtcga tgtcgaagac gcccatgcca
     1081 ccacgccgct ggcggctgta gaggtggcgc gggctgcgtt tggccgcgcc gcgccggcta
     1141 atggtgatga tgctgtaggg cgtcggcagc tcgtcgggta gcgcggcggc gcgcggcggc
     1201 tcgacccggc cgcgctggtg ggccagctcg gcctccagcg cctcgatgta ggcgacgacg
     1261 tcggccgaca cgccgctcag gtcggggcgc tcggtggtca ccaatccccc ccttcaacga
     1321 cgacacagac gggcagcagc gtgcccatat cgaccggatg gcgctcgaag ttggcgaagg
     1381 tcaaatcgcg gaaaggcaaa ctgaaggtgc tgcgggccag ttccattagc gccagatagt
     1441 tggccaacac gcgccgctcc cagtccagac ccagcgctgc cgccgcggcc ttcaaggatt
     1501 tgtcagacgg gccttctagt tccacaaacg tgccgaacgg catctcgtcg agaaccactt
     1561 ccacgtcgcc ccagtggtag gtctggcgat acttctcata ggtctgcacc gggccaaagc
     1621 ccagccgcgc gaggataagg gccatgcggt cgaaatcggc gacctccagc tccaactcct
     1681 cgcggatctt ggcctcactg gcggcgtctt gggcggccag ccccttatag gtcaggcgga
     1741 cgcgcgtgtc ctggcgcaag cgcagtagcg cctgccgggc cagcaagctc tcgtcggccg
     1801 tgtcgaagcg cacgttacgc tcgtgcaccc gcggcgcgcc atctgtcgcg cccgcgcccg
     1861 ccagccgtcg tcgcacgacg tctagatcgg ccacgaggaa tttgacttcg acctccaggt
     1921 ggtcggccgc gggcggcgat gccaacgcgc cggacacgat atcaccatcg tccaggggcg
     1981 cgttcatgag gctgacccga tgccgcccga ttcctggctg atgcccagga gaggctggtc
     2041 tttctcgacg ctggtccccg cagcgacatg gacgtgggtc acgacgccgg cacagggcgc
     2101 gcgcaactcg ttttccattt tcatcgactc cagaatgatg actcgctggc caaactccac
     2161 cgcatcgcca acggccacag gcacagccac aatgatgccg ggcatgggcg aggcgacaac
     2221 ggcctcgcca tcaaccgaca ggccggaggt gcgcatccgg tccaggcgat aggcccgctc
     2281 gtcctgcacc tgcacgttgt acagctcccc ctggataagc acatcccact gattgccgcg
     2341 ctcctccacc atggcggcga tggagcggtt gttcatgagc agcgacgtga cgccgcccga
     2401 cggcagccgg cgaaaatcga tctcgtaggg ctggtcatcg atcaggatgc cgcggtcggg
     2461 gtcgatctca atggtgtatt cctggccctt cacagtcgcg atgtacttca tcttatctcc
     2521 gcagtcgctc ccagcgtgaa taccatttcc agttgctggt gtcgcgcgca ccgggggcga
     2581 caatctgggc ggcgcgttgg ctctgctcgt gggcggccag tgtagccagc aaggcggcct
     2641 ccaatgtatg cggggccccg cggtcgtcca tgctgaagcg gtcttcgacg aacttggtgt
     2701 cgaattggcc cg
//
LOCUS       DAOSMQ010000441         2703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37686_length_2703_cov_5.033988, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000441 DAOSMQ010000000
VERSION     DAOSMQ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37686_length_2703_cov_5.033988"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1736
                     /locus_tag="PK829_12525"
     CDS             138..1736
                     /locus_tag="PK829_12525"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU42082.1"
                     /translation="MTIGVIAAGDKETAAAGAEMLRRGGNAVDAAVAAAFASFIAEAG
                     VVHLGGSGIAQLYDARSGQSRVYDFFSVVPGLGSQPPERLDFEAVTIDFGAATQQFYL
                     GRGAVAVPGNIAGLCRLAADYGRLPLADLLAPAIRLARDGVALAPFQADTCALLAPLY
                     THTEGVRRVFAPAGRVIRPGERLHIPDLAETLTALARAGDDYARRGPLARALLADQAA
                     NGGLLTAADLEAYAVLRLAPIRLAYRDHEVRLPPLCSAGGVLTAFTLRLLSAFDVAAW
                     PHGSVGHLQLLVEVMAATTRARAQWDNWLDALGDEAATARLLDERAVAAARDEVRAAL
                     ADRQPSPVAPEPPGPANTSHLSVMDGEGLAVGLTTTAGESAGYIVPGTGYIANNMLGE
                     EDLHPRGFHTRPAGQRIPTMMTPAVILHGGRPRLVVGSGGSARIRSAIMQTLSNLLDY
                     RLPLDDAVNRARVHLDGDVLQCEAGYDPRAVDALEGMGYRVNRWPARSIYFGGAHSVA
                     RDDDGRLWAAGDNRRGGATAHVEG"
     gene            1740..>2703
                     /locus_tag="PK829_12530"
     CDS             1740..>2703
                     /locus_tag="PK829_12530"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HOU42083.1"
                     /translation="MRRDEILPLGLLLLAYLALAVLFAMRTPAWQAPDEPAHYNYVRQ
                     LVEGNLPVIAPGDWDQAYLGQIVGAEFDPAYSVAGLTYEDWQPPLYYLLQAPLYRLSG
                     GALTAMRLLSVALGAGVVALAYVVGRATFGGRVPPALAVAAFVAFLPQHLAILASVNN
                     DSLAELLIAAILALLVVPWPEPAARGDGRRLLALGLLLGAAFLTKGTAYLMAPVAGAF
                     LLARHWGAWRPLARALLLVFVPALLLGLLWWGRNLAVYGGLDALGKSAHDAVVVGQPR
                     TSEWIAQYGLGGTAGRFLRTTFVSFWGQFGWMAAPLPGWMVAGLA"
BASE COUNT          309 a         1024 c          976 g          394 t
ORIGIN      
        1 gcgcggcgag ccgggggccg ttcttccata tgcgcatatg cggttgcccg acggccaagc
       61 accgcaacgt atgcgccggg gcggagttcg gctacactgg tgggcagccg ggccactcgc
      121 ccggcacagg agcacccatg actatcggag tcatcgccgc cggagataaa gagacggccg
      181 ccgccggggc ggagatgttg cgccggggcg gcaatgccgt cgacgccgcc gtggccgccg
      241 cgttcgcctc gttcatcgcc gaggccggcg tcgtccacct gggcggcagc ggcatcgccc
      301 agctctatga cgcccgcagc ggccagtcgc gcgtctatga cttcttcagc gtcgtgcccg
      361 gcctgggcag ccagccgccg gagcggctcg acttcgaggc cgtgaccatc gacttcggcg
      421 cggccacgca gcagttctat ctcggccgcg gcgccgtggc cgtgccgggc aacatcgccg
      481 ggctgtgccg gctggcggct gactacggcc gcctgccgct ggccgacctg ctggccccgg
      541 ccattcgtct ggcccgcgac ggcgtcgccc tggccccctt ccaggccgac acctgcgccc
      601 tgctggcccc gctctacacc cacaccgagg gcgtccgccg cgtcttcgcc cccgccggcc
      661 gcgtcatccg ccccggcgag cggctgcaca tccccgacct ggccgaaacg ctgacggcgc
      721 tggcccgcgc gggcgacgac tacgcccgcc gcggcccgct ggcccgcgcc ctgctggccg
      781 accaagccgc caacggcggg ctgctgacgg ccgccgacct ggaggcttac gccgtcctgc
      841 gcctcgcgcc catccgcctg gcctaccgcg accacgaggt gcggctgccg ccgctgtgct
      901 cggccggcgg cgtgctgacg gctttcacgc tgcggctgct gtcggccttc gacgtggccg
      961 cctggcccca cggctcggtc gggcacttgc agctcctggt cgaagtcatg gcggcgacga
     1021 ctcgcgcccg cgcccagtgg gacaactggc tcgacgcgct gggcgacgag gcggccacgg
     1081 cccgactgct ggacgagcgc gcggtggccg ccgcccgcga cgaggtgcgc gccgccctgg
     1141 ccgaccggca gccgtcgccc gtcgccccgg agccgcccgg cccggccaac accagccacc
     1201 tgagcgtcat ggacggcgag gggttggccg tgggcctgac gacgacggcc ggcgagtcgg
     1261 ccggctacat cgtccccggc acgggctaca tcgccaacaa catgctaggc gaggaagacc
     1321 tgcacccgcg cggcttccac acgcgcccgg cggggcagcg catcccgacg atgatgacgc
     1381 cggcggtcat cctacatggc ggccggccgc ggctggtcgt cggcagcggc ggcagcgccc
     1441 gcatccgcag cgccatcatg cagacgctca gcaacctgct cgactatcgc ctgccgctcg
     1501 acgacgccgt caaccgggcg cgcgtccatc tggacggcga cgtgttgcag tgcgaggcgg
     1561 gctacgaccc gcgggcggtc gacgcgctgg agggcatggg ctatcgtgtc aaccgctggc
     1621 cggcgcggag tatctacttt ggcggggcgc atagcgtcgc gcgcgatgac gacggccgcc
     1681 tttgggccgc cggcgacaac cggcgcggcg gggccaccgc ccacgtcgag ggctgagcca
     1741 tgcgccgcga cgaaatcctc ccgctcggcc tgctcctgct ggcctatctg gccctggccg
     1801 tcctctttgc catgcgcacg cccgcctggc aggcgcccga cgagccggcc cactacaact
     1861 acgtccgcca actcgtcgag ggcaacctgc ccgtcatcgc gccgggcgac tgggaccagg
     1921 cctacctggg ccagatcgtc ggcgcggagt tcgacccggc ctattccgtg gccgggctga
     1981 cctacgagga ctggcagccg ccgctctact acctgctgca agcaccgctc taccggctgt
     2041 cgggtggcgc gctgacggcc atgcggctgc tgtcggtggc cctgggcgcg ggcgtggtgg
     2101 cgctggccta cgtcgtcggc cgggccacgt tcggcgggcg cgtgccgccg gcgctggccg
     2161 tggccgcctt cgtcgccttc ctgccgcaac atctggccat cctggcttcc gtcaataacg
     2221 acagcctggc cgagctgctc atcgccgcca tcctggcgct gctggtcgtg ccctggccgg
     2281 agccggcggc gcggggcgac ggccggcggc tgctggcgct ggggctgctg ctgggcgcgg
     2341 ccttcctgac caagggcacg gcttacctga tggcccctgt cgctggcgcg ttcctgctgg
     2401 cgcggcattg gggcgcgtgg cggccgttgg cgcgggcgct gctgctggtc tttgtgccgg
     2461 cgctgctgct ggggctgctg tggtgggggc gcaacctggc tgtctatggc gggttggacg
     2521 ccctgggcaa gtcggcccac gacgccgtcg tcgtcggcca gccgcgcacg agcgagtgga
     2581 tcgcccaata tggcctcggc gggacggccg gccgcttcct gcgcacgacc ttcgtcagct
     2641 tctggggcca attcggctgg atggccgcgc cgctgccggg gtggatggtg gccgggctgg
     2701 cgg
//
LOCUS       DAOSMQ010000442         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37708_length_2702_cov_3.750283, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000442 DAOSMQ010000000
VERSION     DAOSMQ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37708_length_2702_cov_3.750283"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            660..>2702
                     /locus_tag="PK829_12535"
     CDS             660..>2702
                     /locus_tag="PK829_12535"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOU42084.1"
                     /translation="MAEDNPQESAVSGERAWLAALLLRRQSRLMPRFALAMARLRSLS
                     RGQRRRLQRRAAASLAGAALVLAIGAANFGPLGAGVAQAATISVANGEIAVNDNGVCS
                     LIEAIINAEDGAQTHDDCLAGTAGADTIDLPAGGAFTFSTPYVYAAGNNGLPAIDTAI
                     TIDGNGATLDLGGPTIPIRLAQVNPTGDLTINATTIRNGLMSDGIYGGGALRVDGGTL
                     TIDGSTLSDNITTAYGGAVFARNDATLTITNSTLSGNRAFRGGAVMGRNSAVTLTDSA
                     VDGNVAKRSGGGIQVEDGRLTLTGGTVNDNGWEPGQIGVVGYVDYAGGVYADGATVSI
                     TGTEIDGNYGGYIGGGVLLADVTGATITGATITGNTADTGGGVAWGRASDGIPFPTTG
                     VIRDSLIDGNTADIGGGAALILGELAIEQTTVSNNTALDGYGYGSGGGVANLGGDLTI
                     TQSALTGNESDYGGGLFAYSYFGGDSTVVTNTTLSGNSASKNGGGVALDRQPEATFNN
                     VTISDNEAADGGGLAATNPPAAGGLGSVTLNRTLIAGNRASATGDEVLVDDVTVTANN
                     YNLFGHSGITNGNAFNSFTPGATDITATSDGTTPTALAGILAALANNGGPTTPPTKTH
                     ALVSNSPALDRAPNAACLAPSPTDGVDQRDVARNQNGTGAAGPNECDVGAYEYVAAP"
BASE COUNT          496 a          937 c          907 g          362 t
ORIGIN      
        1 gccggcgacc ggagcgcccc gggtggacgg ggaaccacca gtcagggaac cagcggctac
       61 accgtccact gccagagtac catacccggt agctaaataa cgccacaaat gggaaaactg
      121 gcccgccact ggtcgggcgc gggccgccaa gggtttttca gtagcacagc ctgtagcaca
      181 gcctgtagca cagcctgtca ggctgtggct gtggctgtgg ctgtggctgt ggctgtggcc
      241 ggcgaggaca gcctaacagg ctgtgctact ttgcgcttcg cgcagttcga cgcccggcct
      301 gtagcacagc ctgtcaggct gtggctgtgg ctgtggctgt ggctggcgag gacagcctaa
      361 caggctgtgc tacaggccgg gcgcgggtcg tcggggcttt gccaaagtcg ctaaaagtgc
      421 gctgacctga caggtgggcg gccaaactct ctctggtgca aaggaaactt caggccgccc
      481 acctgtcagg tcttcgagca attgacgact ttggaaaagc cctggcgggc catcctgccc
      541 attgacaggc gcgtgattgt ctgtatcata ccagacacga aggaaggagc tgcgcagtac
      601 ctctgcgcgg ctcctttgcg tctgtagcag tcgtacttct aaaatcacag gaggttgaca
      661 tggcggaaga caacccacag gaatccgcgg tgagtggcga gcgggcctgg ctggcggcgc
      721 tgctgctgcg gcggcagagc cggctgatgc cccggttcgc gctggcgatg gcccggctgc
      781 ggagcctctc gcgcgggcag cggcggcgct tgcagcggcg ggcggcggcg tcactggccg
      841 gggcggcgct ggtgctggcc atcggcgcgg ccaacttcgg tccattgggc gcgggcgtgg
      901 cccaggcggc gacgatcagc gtcgccaacg gcgaaatcgc cgtcaacgat aacggcgtct
      961 gctcgctgat cgaggcgatc atcaacgccg aagacggcgc ccagacccac gacgactgtc
     1021 tggccggcac ggccggcgca gacaccatcg acctgcccgc gggcggcgcc ttcaccttca
     1081 gcacgcccta cgtttacgcc gcgggcaaca acggcctgcc ggccatcgac acggccatca
     1141 ccatcgacgg caacggcgct acgcttgacc tgggcgggcc aaccatcccc atccgcctgg
     1201 cgcaggtcaa ccccacgggc gacctgacga tcaacgccac gaccatccgc aacggcttga
     1261 tgagcgacgg catctatggc ggcggcgcgc tccgggtcga tggcggcacg ctgaccatcg
     1321 acggcagcac gctgagcgac aacatcacca ccgcttacgg cggcgcggtc ttcgcccgca
     1381 acgacgccac gctgacaatc accaacagca ccctctccgg caaccgcgcc tttcgcggtg
     1441 gggcggtgat ggggcggaac agcgccgtga cgctgaccga tagcgccgtc gatggcaacg
     1501 tggccaaacg cagcggcggc ggcatccagg tcgaggacgg ccgcctgacg ctgaccggcg
     1561 gcacggtcaa cgacaacggc tgggagccgg gccagatcgg cgtggtcggc tacgtcgatt
     1621 acgccggcgg ggtctacgcc gatggcgcga cggtgagcat taccgggacg gagatcgacg
     1681 gcaactacgg cggctacatc ggcggcggcg tgctgctggc cgacgtcacc ggcgcgacca
     1741 tcaccggcgc gaccatcacc ggcaacacag ccgacacggg cggcggcgtc gcctggggcc
     1801 gggccagcga cggcatcccg tttccaacga cgggcgtcat ccgcgacagc ctgatcgacg
     1861 gcaacacggc cgacataggc ggcggcgcgg ccctgatcct cggcgaactg gctatcgagc
     1921 agacgacggt cagcaacaac accgctctgg acggctacgg ctatggcagc ggcggcggcg
     1981 tcgccaatct gggcggcgac ctgaccatca cccagagcgc gctgacgggc aacgaatcgg
     2041 actatggcgg cggtctgttc gcctacagct acttcggcgg cgacagtacc gtggtcacca
     2101 acacgaccct cagcggcaac agcgccagca aaaacggcgg cggcgtggcg ctggacaggc
     2161 agccggaggc gacgttcaac aacgtcacca tcagcgacaa cgaggccgcc gatggcggtg
     2221 ggctggccgc gaccaacccg cccgcggctg gcggcctggg cagcgtgacg ttgaaccgga
     2281 cgctgatcgc tggcaaccgc gccagcgcca cgggcgacga agtcctggtc gatgacgtga
     2341 ccgttacggc caacaactac aacctcttcg gccacagcgg catcaccaac ggcaatgcat
     2401 tcaacagctt cacccccggc gcgaccgaca tcaccgccac cagcgacggc acgacgccaa
     2461 cggccctggc cggcatcctg gccgcgctgg ccaacaacgg cgggccgacg acgccgccga
     2521 cgaagaccca cgccctggtg agcaacagcc cggccctcga ccgcgccccc aatgccgcct
     2581 gcctcgctcc ctcgccgacc gacggcgtcg atcagcgcga cgtggcccgc aaccagaacg
     2641 gcactggcgc ggccggcccc aacgagtgcg acgtcggcgc gtatgagtac gtcgccgcgc
     2701 cg
//
LOCUS       DAOSMQ010000443         2692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37889_length_2692_cov_3.950322, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000443 DAOSMQ010000000
VERSION     DAOSMQ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37889_length_2692_cov_3.950322"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1330)
                     /gene="nrfD"
                     /locus_tag="PK829_12540"
     CDS             complement(38..1330)
                     /gene="nrfD"
                     /locus_tag="PK829_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOU42085.1"
                     /translation="MTKIATIKTFVRNTVLPALRQPVIRFMLGLGVLMAIGVGAALVV
                     FAEGLHVTNLTDLVPWGLWISIDLSSIALAAGAFSISAIAYLLHRKELRPVATTAVYV
                     GFVCYSIAMMMLLLDIGRPDRFWHGFVFWNIHSPLWEVTMCVGLYFTVLLMEVAPIIG
                     HWEPVEHRFPRLSHRLGSLHALTPYLALIGLCLSTLHQSSLGLTYGNLIARPIWSRPI
                     PMAVSFYLSAIVGGIALVTCISFLAPHLTPRARVNKVTLDKLAYTVGWAALFLLVVRW
                     WDLMSMPVNYEPAKSEALYILTRGRLAFNFWGLEILLGMVVPAILLLVGSFRRNERAR
                     ILALALVALGLVAYRWDTNLVGQLVVFGQAPGSDVPLFTNYTPSLIEILTATGVIAYG
                     LLAVTFGVRYLNVIDHGEVVEAPAEAPVPAAMIPSSSR"
     gene            complement(1327..2211)
                     /locus_tag="PK829_12545"
     CDS             complement(1327..2211)
                     /locus_tag="PK829_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU42086.1"
                     /translation="MSSIDRRDFLKLGLAATATAAVGVGMSRIPLPMAAGEPVVGAGF
                     KSAPTGDTEPEIIRADEAAALPRADHDQPSPHHWAMVIDQGKCVGCDLCLAACHAYND
                     TPPNISWSRVEEVAPTADGRRVFRPTPCMHCEDAPCVEICPVGATYHRYDGLVMMDYD
                     RCIGCRYCQLACPYGVRHFNWEAFTGPNPDVPAAGHPEVPRRPRGVVEKCSFCVQRID
                     RGLAAGLTPGRDPDATPACVVACVYGARTFGDLNDPESMVSIALRQRPDAYRLREELG
                     TGPRVYYLPATPTEVSES"
     gene            complement(2214..>2692)
                     /locus_tag="PK829_12550"
     CDS             complement(2214..>2692)
                     /locus_tag="PK829_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42087.1"
                     /translation="SCHNTEGHYYTFTGHAAEGLLCTDCHLRISDSPEAGTRGHGARS
                     HTFQIDLESCNDCHLSDMHAEVDSAMAATTAVEGQAQTDVACYPVETTDIQADNFVPA
                     REEVTPYVSQTPSGPGPLAYLLPAGIGLVFGTMVAPWVNRWTKRRHSDSDSEQGEK"
BASE COUNT          477 a          819 c          984 g          412 t
ORIGIN      
        1 cgcggcgctt ttctacccac cctttttcct gagttgctca acgggaggag gaagggatca
       61 tggcggcggg cacaggggct tcggcgggcg cctccaccac ttctccatga tcaatcacat
      121 tgagataccg gacaccgaat gtgaccgcca gcaggccgta ggcgatcaca ccggttgctg
      181 tcaggatctc aataagtgat ggcgtatagt tggtgaacag cggcacgtcg ctgccgggcg
      241 cttgcccaaa gacgaccagc tgtccgacga gattggtgtc ccagcgatag gcgacgaggc
      301 ccagggccac cagggccagc gccaggatgc gcgcgcgctc gttgcgccgg aaggagccga
      361 cgagcagcag tatggcgggc acaaccatgc ccagcagaat ctccagcccc cagaagttga
      421 acgccagccg gccgcgcgtc aggatgtaga gcgcctccga cttggccggc tcgtagttca
      481 ccggcatcga catcaggtcc caccagcgca cgaccagcaa gaagagcgct gcccagccga
      541 cggtataggc cagcttgtcg agagtgactt tgttgacgcg cgcccgcggc gtcaggtgcg
      601 gcgcgaggaa cgaaatgcac gttaccaggg cgatgccgcc gacaatggcc gacaggtaga
      661 aggagacggc catcggaatg gggcgcgacc agatgggtcg ggcgatgaga ttgccgtagg
      721 tcaggcccag cgacgactga tgcagcgtcg agaggcacag gccaatgagc gccaggtaag
      781 gcgtcagggc gtggagcgag ccgaggcgat gggagaggcg cgggaagcgg tgctcaaccg
      841 gctcccaatg gccgatgatc ggcgcgactt ccatcagcag gacggtgaag tacaggccga
      901 cgcacatcgt cacttcccat agcggcgagt ggatgttcca gaagacgaag ccatgccaga
      961 agcggtcggg ccggccgatg tcgagcagga gcatcatcat ggcgatgctg tagcagacga
     1021 agccgacgta gacggccgtt gtagccacgg ggcgcagttc cttgcgatgt agcagatagg
     1081 cgatggccga gatgctgaac gcgcccgccg ccagagcgat ggacgacagg tcgatggaaa
     1141 tccacagccc ccacggcacc aggtcggtca gattggtcac atgcaggccc tcggcgaaga
     1201 ccaccagggc cgcgccgacg ccgatagcca tcagcacgcc caggccgagc atgaagcgga
     1261 tgaccggctg gcgcagggct ggcaagaccg tgttgcggac gaaggtcttg atggtggcga
     1321 ttttggtcat gactcgctga cctccgttgg agtagcgggc aggtagtaga cgcgcggccc
     1381 ggtgcccagt tcttcgcgca ggcggtaggc gtcgggacgc tgccgcaggg cgatgctgac
     1441 catcgactcc gggtcattca ggtcgccgaa ggtgcgcgcg ccgtagacgc aggcgaccac
     1501 gcaggccggc gtggcatcgg ggtcgcggcc gggcgtcagg ccggcggcca ggccgcggtc
     1561 gatgcgctgg acgcagaagg agcacttctc gacgacgccg cgcggccggc ggggcacttc
     1621 ggggtggccg gcggcgggca cgtcggggtt ggggccggtg aaggcctccc agttgaagtg
     1681 gcgcacgccg taggggcagg ccagctggca gtagcggcag ccgatgcagc ggtcgtagtc
     1741 catcatcacc aggccgtcgt agcggtggta cgtggccccg acggggcaaa tctcgacgca
     1801 gggcgcgtcc tcacagtgca tgcacggcgt cgggcggaag acgcgccggc cgtcggccgt
     1861 cggcgccacc tcttcgacgc gcgaccagga gatgttgggc ggcgtgtcgt tgtaggcgtg
     1921 gcaggcggcc aggcacaggt cgcagccgac gcacttgccc tggtcgatga ccatcgccca
     1981 atgatggggc gagggctgat cgtggtcagc gcgcggcagc gcggcggctt cgtcggcgcg
     2041 gatgatttcc ggctcggtat cgccggtagg ggcggatttg aaacccgccc ctacgacagg
     2101 ttcaccggcc gccatcggca gtgggatgcg gctcatccct acgccgacgg cggcggttgc
     2161 cgtggcggcc aggccgagct tcaagaaatc gcggcggtcg atggacgaca tctctacttt
     2221 tctccctgct cgctgtcgct gtcgctgtgg cggcgctttg tccagcggtt gacccacggc
     2281 gccaccatcg tgccgaacac caggccaatg ccggccggca gcaggtaggc caatgggccg
     2341 gggccgctgg gcgtctggct gacgtagggc gtcacttcct cgcgcgccgg gacgaagtta
     2401 tcggcctgga tgtcggtcgt ctcgaccggg tagcaggcca cgtcagtctg cgcctgcccc
     2461 tccacggcgg tggtggcggc catagccgaa tcgacttcgg catgcatgtc gctcaggtgg
     2521 cagtcgttgc acgactccag gtcgatctgg aaggtgtggg agcgcgcgcc gtggccgcgc
     2581 gtgccggcct ccggcgagtc ggagatgcgc aggtggcagt cggtgcagag cagaccctcg
     2641 gcggcgtggc cggtgaaggt gtagtaatgg ccctcggtgt tgtggcagct gt
//
LOCUS       DAOSMQ010000444         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37932_length_2689_cov_4.770691, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000444 DAOSMQ010000000
VERSION     DAOSMQ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37932_length_2689_cov_4.770691"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..140
                     /locus_tag="PK829_12555"
     CDS             15..140
                     /locus_tag="PK829_12555"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOU42088.1"
                     /translation="MKGVDKVLDAAGWTYVAAAVAAVGQLLYYVLLLGGGGRRRS"
     gene            complement(222..1118)
                     /locus_tag="PK829_12560"
     CDS             complement(222..1118)
                     /locus_tag="PK829_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU42089.1"
                     /translation="MDAYVGGIEAGGTKVVCAVGSGPDDVRAEARFPTTAPAETIGRA
                     LAFFREQEAHYGPVGAIGIAAFGPLDAHPHSPRFGTITSTPKPGWANADVVGPFREAL
                     GVPVGFDTDVNIAGLGEWRWGAAQGLENFIYLTIGTGVGGGGMINGQLLHGLVHPEFG
                     HIPVPHDWAADPYKGRCPYHGDCLEGMAAGPAIGERWGRPAYELLPEHPAWELEAHYL
                     GLALQTFICTLSPERIILGGGVMEQPQLFPLVRRQTQAYLNGYVQSPVILTDIDSYIV
                     PPALGNRAGVLGAFAQAQLLLG"
     gene            complement(1242..>2689)
                     /locus_tag="PK829_12565"
     CDS             complement(1242..>2689)
                     /locus_tag="PK829_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU42090.1"
                     /translation="LRAEGISLCIDLVCNHTAKEHEWARRAAAGDPTYQAYYLMYPDR
                     AEPDAYERTLPEVFPDFAPGNFTYYPAIDRWVWTTFNEYQWDLNYGNPAVLAEMLDII
                     LYLANQGVEVLRLDAVAFMWKRLGTDSQNQPEAHSILQAWRALSRIAAPGLLLKAEAI
                     VSPPKLIPYLGRGEATNKECELAYHNVFMVLLWSALAEGRVALMTRALQNMPPIPSGA
                     AWITYVRCHDDIGWAVTDEDAAEVGLSGPGHRAFLSDFYSGRFANTFARGATFQFNPR
                     TNDRRISGSCASLAGLERALEGKDAPAAETAIRRILLVYSLIFAFGGIPLLYMGDELG
                     LLNDAGYLDDANLADDNRWMHRPRMDWARAEERRDWWTVAGRIFRGLSEQLAARRDTA
                     ALHAQAGAFPVWTHNDAVFGLLRDSPRGRVLVLANFSDRPQSVPGYRLHEMLFGGRLV
                     DRLTGRVMDSAPGITLERYEALWLTPEAELG"
BASE COUNT          426 a          911 c          913 g          439 t
ORIGIN      
        1 tgggcaacga cgagatcaag ggcgtggaca aggtgctcga cgccgccggc tggacctacg
       61 tggccgccgc cgtcgccgcc gttggccaac tgctgtacta cgtactcctg ctgggaggcg
      121 gcggccgacg ccgcagttag ctcctgccag agcaacattc tccagaaacc aggacgccag
      181 ctatcaagct ggcgtctttt tgtatcctgt gagcgcggct gtcagcctag cagcaactgc
      241 gcctgcgcaa aggcccccaa cacccctgcc cggttgccca gcgccggcgg cacgatgtag
      301 ctgtcgatgt ccgtcaggat gaccggcgac tggacgtagc cgttcaggta ggcctgtgtc
      361 tggcggcgca ccagcgggaa gagctgcggc tgctccatga ccccgccgcc gaggatgatg
      421 cgctccggtg acagggtgca gatgaacgtc tgcaacgcca ggcccagata gtgcgcctcc
      481 agttcccagg cgggatgctc cggcagcaac tcataagccg gccgccccca gcgctcgccg
      541 atggccgggc cggcggccat accctctagg caatcgccgt ggtaagggca gcgccccttg
      601 taggggtcag cggcccagtc gtggggcacg gggatgtgac cgaactccgg atgcaccagc
      661 ccgtgcagca actggccgtt gatcatccca ccgccgccca cgcctgtgcc aatggtcagg
      721 taaatgaagt tctccagccc ctgcgccgcg ccccagcgcc actcccccag cccggcaata
      781 ttgacgtcgg tgtcgaaccc gacgggcacg cctagcgcct cccggaacgg cccgaccacg
      841 tcggcgttgg cccagcccgg cttgggcgta ctggtaatgg tgccgaagcg gggcgagtgg
      901 gggtgggcgt ccagcgggcc gaaggcggcg atgccgatcg caccgacagg gccataatgc
      961 gcctcctgct cgcggaagaa ggccagggca cggccaatgg tttccgccgg tgccgtcgtc
     1021 gggaagcgcg cctcggcgcg cacgtcgtcc ggcccgctgc cgacggcgca cacgaccttc
     1081 gtaccgccgg cctcgatgcc gcctacgtat gcgtccatgt caatcactcc ttatggtggg
     1141 aagaggctca cgcaaagacc gcaaagggtg ctaagttcgc aaagaggatt tctcttggcg
     1201 ttctttgctc gcttggcgtt ctttgcgtga gcctcttttc ttcatcccag ctccgcctcc
     1261 ggcgtcagcc acagcgcttc ataccgctcc agggtgatgc ccggcgcgct gtccatgacc
     1321 cgcccggtca ggcgatccac cagccggccg ccgaagagca tctcgtgcag ccggtagccg
     1381 gggacgctct gtggccggtc gctgaagttg gccagcacca acacgcggcc gcgcgggctg
     1441 tcgcgcagca ggccgaagac ggcgtcgttg tgggtccaga cgggaaacgc gcctgcctgg
     1501 gcgtgcagcg cggccgtgtc gcggcgcgcg gccagttgct cgctcaggcc gcgaaagatg
     1561 cgccccgcca ccgtccacca gtcgcggcgc tcctcggccc gcgcccagtc catgcgcggc
     1621 cggtgcatcc agcggttgtc atcggccagg ttggcgtcgt ccaggtaacc ggcgtcgttg
     1681 agcaatccca gctcgtcgcc catgtagagc agcgggatgc cgccgaaggc gaagataagg
     1741 ctatagacca gcaggatgcg gcggatggcc gtctcggccg cgggcgcgtc tttcccctcc
     1801 agcgcccgct ccagcccggc cagcgacgcg cacgagccgc tgatgcgccg gtcgttggtc
     1861 ctggggttga actggaaggt cgcgccgcgg gcgaaggtgt tggcgaagcg tccgctatag
     1921 aaatcgctca gaaaggcgcg gtggccgggg ccggatagcc ccacctcggc ggcgtcctcg
     1981 tcggtgacgg cccagccgat gtcgtcgtga cagcgcacgt aggtgatcca ggctgcgccg
     2041 gacgggatgg gcggcatgtt ctgcaacgcc cgcgtcatca gggccacgcg cccctcggcc
     2101 aacgcgctcc acagcagcac catgaagacg ttgtggtagg ccagctcgca ttccttgttg
     2161 gtcgcctcgc cccggcccag ataggggata agcttcggcg gcgagacgat ggcctcggct
     2221 ttcagcagca gccccggcgc ggcgatgcgg ctcagggcgc gccaggcttg caggatggag
     2281 tgggcttcgg gctggttctg gctgtccgtg cccagccgct tccacatgaa ggcgacggcg
     2341 tccagccgca gcacctcgac gccctgattg gccaggtaga ggatgatgtc gagcatctcc
     2401 gccaggacag ccgggttgcc atagttcagg tcccactggt actcgttgaa cgtcgtccac
     2461 acccagcggt cgatggcggg gtaataggtg aagttgcccg gcgcgaagtc ggggaagacc
     2521 tccggcagcg tgcgctcgta ggcgtccggc tcggcgcggt cggggtacat caggtagtaa
     2581 gcctgatagg tcgggtcgcc ggccgcggcc cgccgtgccc attcgtgctc cttggcggtg
     2641 tggttgcaga ccaggtcgat gcacaggcta atgccctcgg ctcgcagct
//
LOCUS       DAOSMQ010000445         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37934_length_2689_cov_4.634776, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000445 DAOSMQ010000000
VERSION     DAOSMQ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37934_length_2689_cov_4.634776"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK829_12570"
     CDS             complement(<1..414)
                     /locus_tag="PK829_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOU42091.1"
                     /translation="MKRALKWAAIMIVGLVGIVVLIALTGYIVSERRIAKTYAIADEV
                     LTLPADAASLTEGQRLVTIRGCGDCHTPDLGGQVLLDDPMLGTIPTANLTRGQGSATA
                     GYSVADWERAIRHGVGPDGHSLLFMPSYEFSGLSDE"
     gene            complement(435..1445)
                     /locus_tag="PK829_12575"
     CDS             complement(435..1445)
                     /locus_tag="PK829_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42092.1"
                     /translation="MNGKRTSSVPSAFLAIDDRRVVRAACGADGNWQVDCVLDGPAPV
                     TLAADPSSPDRLYAGCQGHGVFTSADQGQTWHSLGLAGRVVKAIAVSPHDPRTIYAGV
                     KPAALYKSTDGGQSWLELEGFRRIPFRWWWFSPAESPFRAYVNALAVSPTEPEVVLAG
                     IELGAVVRSEDGGRSWSRHRPGALRDCHSLGFHQRDGRWVYEAGGTGGGAAVSRDGGR
                     AWNQPKSGLAKKYGVTCAADPERPEVWYVALAPSPARAYGKDAAAYLYRASAGAGWEP
                     IGWEPHPLGQMPRALVTRPGAPGHLFAGTTYGDVWHTADYGDTWQRLPFNLGGGLSLL
                     VL"
     gene            complement(1496..1918)
                     /locus_tag="PK829_12580"
     CDS             complement(1496..1918)
                     /locus_tag="PK829_12580"
                     /inference="COORDINATES: protein motif:HMM:NF040028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HOU42093.1"
                     /translation="MKVARMLYWVFAWLFVVGVATQVFFAGMAVVANRWGWDNHIGLG
                     HTLAAPLLVMLVTMVLGKLPGRMKRLTWLLFGVYVLQADVLIFLRASAPVLSALHPVM
                     ALVDFALGLALARQARALVRADEASELARPHAEGASAD"
     gene            complement(1951..2607)
                     /locus_tag="PK829_12585"
     CDS             complement(1951..2607)
                     /locus_tag="PK829_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42094.1"
                     /translation="MTVSDSPFVGWTAIAGGVVGVVGFIALVLLFVVGEPFGTLNDVL
                     SIPVALLAAPLLFALYQLHAASYPAVAVVALLAGLAGYAAAAIGSGLLVLGRIDFNTS
                     LLFGIGGFGLIGLWALLTSGMGLRGDTLPRAAAWAGLALSVTPTLALLTVLRAESVAN
                     ALNAMGGQTGGTPPGALAMVFVVLGGLSYAAMPLWIIWLGRLFLMGRVGLPAAVALGQ
                     "
BASE COUNT          534 a          943 c          843 g          369 t
ORIGIN      
        1 ctcgtcgctc agcccggaaa actcatagct gggcatgaac aagaggctgt ggccatctgg
       61 gccgacgccg tgacgaatgg cccgctccca atcggcaacg ctgtagccgg cggtggcgct
      121 accctggcca cgggtcaggt tggccgtggg gatagtgccg agcatggggt catcgagcaa
      181 cacctggccg cctaaatcgg gcgtgtgaca gtcgccacag ccgcggatgg tcaccagccg
      241 ctgtccttcc gtcaggctgg cggcgtcggc cggcagcgtc aacacctcat cggcgatggc
      301 gtaggtcttg gcgatgcgtc gctcgctaac gatgtagccg gtcagggcga tcaagacaac
      361 gatcccgacc agcccaacga tcatgatcgc ggcccacttg agtgctcttt tcatatcaaa
      421 tctccaacat cgttctacag aaccagtagc gacaacccgc cgcccagatt gaatggcaac
      481 cgctgccagg tatcgccgta gtcggccgtg tgccacacgt cgccgtaggt tgtgccggca
      541 aagagatggc ccggcgcgcc ggggcgggtg accagcgccc gcggcatctg gcccagcggg
      601 tgcggttccc aaccaatagg ctcccagccg gctccggccg aagcccgata gagatacgcc
      661 gccgcgtctt tgccataagc cctggccggg ctgggagcca aagcgacgta ccagacttcc
      721 ggccgctccg ggtcggcggc acaggtgacg ccatatttct tggccagacc acttttcggc
      781 tgattccagg cgcgcccgcc atcgcgacta acggccgccc cgccgcccgt cccgccggct
      841 tcatagaccc accgcccatc gcgctgatgg aatcccaggg agtgacagtc gcgcagagca
      901 ccggggcgat gccgcgacca ggagcgtccg ccatcctcgc tacgcacaac cgcgcccagc
      961 tcgatgccgg ccaggacgac ctccggctca gtgggcgaaa cggcgagggc gttgacgtag
     1021 gcccggaagg gcgactcggc cggcgaaaac caccaccagc gaaacggaat gcgccggaag
     1081 ccttccagtt caagccagct ttggccgccg tccgtggact tgtacagggc ggcgggcttc
     1141 actccggcat agatcgtccg ggggtcgtgg gggctgacgg cgatcgcctt cacgacccgt
     1201 ccggccaggc ccaggctatg ccatgtctgg ccctggtcgg ccgatgtgaa aacgccatga
     1261 ccctggcagc cggcgtatag ccggtcgggg ctgctcggat cggccgccag cgtcaccggc
     1321 gccggcccgt ccaggacaca atcaacctgc cagttgccgt ctgccccgca cgccgcgcgg
     1381 acaacgcgcc ggtcatcaat ggccagaaaa gcggaaggga cgctgctggt tcgtttgccg
     1441 ttcatgttcg acctctagcc catcacaatc ctgataagcc ctgaccggca cgcgcttaat
     1501 cagcgcttgc cccttccgcg tgcgggcgag ccagctccga cgcctcgtcc gcgcgaacca
     1561 gcgctcgcgc ctgccgcgcc aacgccagcc ccagagcgaa atcgaccagg gccatgactg
     1621 ggtgcagcgc cgacaggacg ggcgcgctgg cgcgcaggaa gatgagcacg tcagcctgca
     1681 agacgtagac gccgaacagc agccacgtca gccgcttcat ccggccgggc agcttgccca
     1741 gtaccatcgt caccagcatg accaggagcg gcgcggccag ggtgtggccc aggccgatgt
     1801 ggttgtccca cccccagcga ttggccacga cggccatgcc ggcaaagaag acctgcgtag
     1861 ccaccccgac aacgaacagc caggcaaaga cccagtagag catacgcgca actttcatga
     1921 caatacctcc aaccaatcgt tatgagccaa ctactgaccg agggccacag ccgccggcaa
     1981 tcccacccgc cccatcagga acaagcgccc cagccagata atccagagcg gcatggcggc
     2041 gtaactgagg ccgcccagga caacaaagac cattgccagc gcgccaggcg gcgtgccgcc
     2101 cgtctgaccg cccatcgcgt tcaaggcatt ggccacgctt tcggcgcgca acacggtcag
     2161 cagagccagc gtcggcgtca ccgacagggc caggccggcc caggccgccg cccggggtag
     2221 cgtgtcgccg cgcaggccca tgcccgacgt caacagcgcc cacaggccga tcagcccgaa
     2281 cccgccaatg ccgaacaata gcgaagtgtt gaagtcgatc cgccccagga cgagcaagcc
     2341 cgatccgatg gccgcggctg cgtagccggc cagcccggcc agcagcgcca caacggcgac
     2401 cgcggggtag ctggccgcgt gaagctggta cagcgcgaac aggagcggcg ccgccagtag
     2461 cgccaccgga atggacagaa cgtcgttcag cgtgccgaac ggctcgccga cgacgaacag
     2521 cagcaccagg gcaatgaagc cgacgacgcc gacgacaccg ccggcgatgg ccgtccaacc
     2581 gacgaaaggc gaatctgata cagtcatacg ctgcgtcctc caatggaatg aatttggcgc
     2641 ggtgttgaga gcattgagtg aagccagtat aggcggtggc ccgtcgggc
//
LOCUS       DAOSMQ010000446         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_37935_length_2689_cov_4.509871, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000446 DAOSMQ010000000
VERSION     DAOSMQ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37935_length_2689_cov_4.509871"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK829_12590"
     CDS             <1..486
                     /locus_tag="PK829_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42095.1"
                     /translation="QEPPRSSEWAPPPMSNLHRQRGDGPGGPVPGHISVDERFGWYMI
                     AGRRGNFLPTIAPGVWLLADSEIDERPASAHDYVVVGSSRAGLGSITAQPISHSTTLP
                     HCYLGYRVVGPDGASQLVVDDSRQAVSGGDLLVLAVVEGFWYALDGQPAAGPPLGGIN
                     G"
     gene            479..1408
                     /locus_tag="PK829_12595"
     CDS             479..1408
                     /locus_tag="PK829_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU42096.1"
                     /translation="MAKRVLVIGATLLDTKGKPLAGLAPGMSNPAEIRSTRGGTARNV
                     AENLARLGADVVLVSAVGDDPTGRQLMMQTAEAGVNLDDVEMVAGAHTGSYIALLEED
                     GRLAVALDDVRVMQTIDAAYLEARHDLFAGAAMVMFDGSLSEEAIAVVVRLARASGAL
                     LCADPSSARLAHKLRPYLPYLHLLVPNEIEEAALCEVGFAGHDPNASLALARRLIGAG
                     VANVVVTLADFGLDYATADEIGYIPPSHTHMVDSTGTGDAVTAAILFGMLNDLSPIEA
                     IRLGAAAAALTLQSPDTVVPDLSLDMLYDHLIV"
     gene            1533..2198
                     /locus_tag="PK829_12600"
     CDS             1533..2198
                     /locus_tag="PK829_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42097.1"
                     /translation="MTNTLGFILCIVYVFGMIGLAEGLRRWRGYGSGFTRKIIHIGVG
                     MMSWFLPFLFTSPWPFVAACGGFMVLNLLDWRYGFFNAMQSSSRSNLGTVYFPLAAAV
                     VALVFWEQPPLMVAALMPLTWGDGLAPVVGAAYGRHVYRIHTSTRSVEGSAGFFVAGF
                     VFTWLALWVMPGVPDISPAQALLPALVIMAITTVIEAVSIWGLDNLAVTAAAILILRV
                     WPF"
     gene            complement(2203..>2689)
                     /locus_tag="PK829_12605"
     CDS             complement(2203..>2689)
                     /locus_tag="PK829_12605"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU42098.1"
                     /translation="PIPAHQTISQPYVVAYMIAALALEPGDRVLEIGAGSGYAAAILS
                     RIVSAVYTVERHPELVSYARERLAALGYSNVYVTQGDGTRGWPEHAPYNGIIVAAGGP
                     SVPESLRAQMADGGRLVIPVGRSRQQQYLIRVTRQADGRAREERLAPVAFVPLIGDEG
                     W"
BASE COUNT          382 a          913 c          890 g          504 t
ORIGIN      
        1 caggagccac cgcgctcgtc ggagtgggcg ccgccaccca tgtccaacct gcatcgccag
       61 cgcggcgacg ggccaggcgg ccctgtgccc ggccacatct ccgtcgatga gcgcttcggc
      121 tggtacatga tcgccggtcg tcgcggcaac tttctgccca ccatcgcgcc cggcgtctgg
      181 ctgctggccg atagcgagat cgatgagcgg ccggcgtcgg cgcacgacta cgtcgtcgtc
      241 ggcagcagcc gcgccggcct gggcagcatc accgcccagc ccatctccca ctccacgacc
      301 ttgccgcatt gctatttggg ctatcgcgtc gtcggccccg acggcgcgtc gcagctggtc
      361 gtcgacgaca gtcgtcaggc ggtcagcggt ggcgatctgc tggtgctggc cgtggtcgag
      421 ggcttctggt atgcgttgga cggtcaaccg gccgcggggc cgccgctggg gggaatcaat
      481 ggctaaacga gtgctcgtta tcggcgcgac gctgctggac accaagggta agcccctggc
      541 cgggttggct ccagggatgt ctaacccggc tgagattcgc tcgacgcgcg gcggcacggc
      601 ccgcaacgtc gctgagaacc tggcccgcct gggggccgat gtcgtcctcg tttccgccgt
      661 gggcgacgac cccaccgggc ggcagttgat gatgcagacg gccgaagccg gggtgaacct
      721 cgacgacgtg gaaatggtcg ccggcgcgca cactggttcc tatatcgcct tgctggagga
      781 ggacggccgg ctggccgtgg ctctcgacga cgtgcgtgtc atgcagacca tcgacgcggc
      841 ctatctggaa gcgcgccacg acctctttgc cggcgcggcc atggtgatgt ttgacggcag
      901 cctgagcgag gaggctatcg ccgtggtcgt gcgactggcc cgcgcgtctg gcgcgctcct
      961 gtgcgccgat ccgtcgtcgg cccgattggc ccacaagctg cgcccctatc tgccctatct
     1021 gcatctgctt gtgcccaacg agatcgagga ggccgccctg tgcgaggttg gctttgccgg
     1081 ccacgacccc aacgccagcc tggccctggc gcgccgcctc atcggtgccg gtgtggccaa
     1141 cgtcgtcgtc acgctggcgg atttcgggct ggattacgcc acagctgacg agatcggcta
     1201 catcccgccg tcgcacaccc acatggtcga tagcaccggc acgggcgacg ccgtcactgc
     1261 ggccatcctg ttcggcatgc tcaacgactt gtcgcccatc gaagccattc gcctgggcgc
     1321 ggccgccgcc gccctaaccc tccagtcccc cgacactgtc gtgccggact tgagtctgga
     1381 tatgttgtat gatcatctga ttgtgtaggg agttgttgta ctgaccgccg accgccggcc
     1441 acagacgaca gaccacagac aaccgccgac tgcccaccga tactgatctg ctgaacactg
     1501 acctactgct tcctcatccc tcatccctca tcatgaccaa cactctcggc ttcatcctct
     1561 gcatcgtcta cgtcttcggc atgatcggtc tcgcggaggg gctgcgccgt tggcgagggt
     1621 atggctccgg cttcacgcgc aagatcatcc atatcggtgt gggcatgatg agctggttcc
     1681 tgccctttct attcacctcg ccctggccat ttgttgccgc ttgcggtggg ttcatggttc
     1741 tcaacctgct cgattggcgc tatggcttct tcaacgccat gcagagcagc agccgcagca
     1801 acctgggcac ggtctacttc ccgttggctg cggccgtcgt cgccctggtg ttctgggagc
     1861 agccgccgct gatggtcgcc gcgctgatgc cgctgacctg gggtgatggg ctggccccgg
     1921 tcgttggcgc ggcctatggg cggcacgtct atcgtattca caccagcacg cgctccgtgg
     1981 aggggtcggc cggtttcttc gtggccggct ttgtcttcac ctggctggcc ctgtgggtca
     2041 tgcccggcgt gcccgacatc agcccggcac aggcgctgct gcccgcgctg gtgatcatgg
     2101 ctattaccac ggtcatcgaa gcagtgtcga tttgggggct ggacaacctg gccgtgacgg
     2161 cggcggccat tctgatcttg cgcgtctggc cgttctaagc gcctaccagc cctcgtcgcc
     2221 gatcagcggc acgaaagcga ccggggcgag ccgttcctcg cgcgcgcggc catcggcctg
     2281 gcgcgtcacg cggatgaggt actgctgttg ccggctgcgg ccgacgggta tgaccagccg
     2341 cccgccgtcg gccatctgcg cccgcagcga ttcaggcacg gacgggccac cggcggcgac
     2401 gatgatgccg ttgtagggcg catgctccgg ccagccgcgc gtgccgtcgc cctgagtcac
     2461 atagacgttg ctatagccga gcgcggccag ccgctcgcgg gcgtagctga ccagttcggg
     2521 gtggcgctcg acggtgtaga ccgcgctgac aatgcggctg aggatggccg cggcatagcc
     2581 cgagcccgcg ccgatctcca gcacgcgatc gcccggctcc agcgccagcg cggcgatcat
     2641 gtaggcgacg acgtaaggct gggagatggt ctggtgggcg gggatgggc
//
LOCUS       DAOSMQ010000447         2679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38115_length_2679_cov_3.798780, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000447 DAOSMQ010000000
VERSION     DAOSMQ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38115_length_2679_cov_3.798780"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="PK829_12610"
     CDS             complement(<1..1438)
                     /locus_tag="PK829_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOU42099.1"
                     /translation="MSTNYRPAAPQPRLSRGRAILAGLLLLAILMLAAGVGRAAARQA
                     SPASDSAGNAAGTSVNNLPLPTTRNDFFLPGTQPSNPDDKKFAEIADPRSCNSCHTAP
                     IYQTWRGSMMGQAGRDPLFWSAFAVANDDATDAGEFCLRCHAPTGWFADRSHPADGSA
                     LTDQDKDAGLACEVCHRMVDPVPGAQDEATTLDIAIRAALTNPPPTNHVASAMIILDP
                     SVDAQGQPLNRRRGPFARPSNPHPALQARFQGQGQDAVTESRLCGSCHNVDNPALSWN
                     PDPPGNAPAQYWPSDFDAAAPSFEKGQLFPLERTYEEWLNSSYARGGVFAPQFAGAML
                     SPVVETCQDCHMERQTGYAANRYGPTYRDCNTTGCLPAHVFVGGNAWIPRILQDSRWE
                     LHNPDDATALQLTGAAAESMLRRAATLTMSLEPSGAGQVATVRVTNETGHKLPTGYPE
                     GRRMWLNLHAYNAAGQLIYESGAYNTTTG"
     gene            complement(1435..2442)
                     /locus_tag="PK829_12615"
     CDS             complement(1435..2442)
                     /locus_tag="PK829_12615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOU42100.1"
                     /translation="MLAFLAAFFIALAITLAGLPWVRRAALALSLVDAPSERKTQRTP
                     MPLLGGVGIFVGAILAVLVIYRGEPEPTVLGVLLATTVVALTGLIDDYRPLPAWAKLA
                     GQLAGCLILAAFGIRVRLPLPAALNYAITLLWLLGITNAINFLDNMDGLSAGLAAVIS
                     SFILLLALTNGQFLVAALSAALLGACVGFLRYNFPPARIFMGDVGSLFLGFLLAAMGI
                     QLRFPDNSNFVTWMVPVFLFGLPIFDMTLVVVSRLRRGLSPNTAGRDHTSHRLVRLGF
                     APREAVLILYLFCGVLGMMALYITQASVVEGYLVGGIAAALALAALVWLERQWKESEA
                     T"
     gene            complement(2461..>2679)
                     /locus_tag="PK829_12620"
     CDS             complement(2461..>2679)
                     /locus_tag="PK829_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42101.1"
                     /translation="GEYEAAATALAHARELNDPVSIPTAELDFLEAQILDGLGRPDEA
                     RALAERALETAGEETAAQIEAFLATLDQ"
BASE COUNT          437 a          837 c          988 g          417 t
ORIGIN      
        1 taccggtggt cgtgttgtag gccccggact cgtagatgag ttgcccggcg gcgttgtagg
       61 cgtgcagatt cagccacatg cgccgccctt cggggtagcc ggtgggcagc ttgtggcccg
      121 tctcgttggt gacgcgcacg gtggcgacct gccccgcgcc gcttggttcc aggctcatgg
      181 tcagcgtggc cgcccggcgc aacatgctct cggccgccgc gccggtcagt tgcagggccg
      241 tcgcgtcgtc cgggttgtgt agttcccaac ggctgtcctg caggatgcgc gggatccagg
      301 cgttgccgcc gacgaagacg tgggcgggca ggcagccggt cgtgttgcag tcgcggtagg
      361 tggggccata ccggttggcg gcgtagccag tctgacgctc catgtggcaa tcctggcacg
      421 tctccacgac cgggctgagc atggccccgg cgaactgcgg cgcgaagacg ccgcccctgg
      481 cgtagctgct gttgagccat tcttcgtagg tgcgctccag cgggaagagc tgccccttct
      541 cgaacgacgg cgcggcggcg tcgaagtcgg atggccagta ctgcgccggg gcgttgccgg
      601 gcgggtcggg gttccagctc aacgccgggt tgtctacgtt atgacaactg ccgcataggc
      661 gcgattcagt cacggcgtcc tgcccctggc cctggaagcg cgcttgcagc gcggggtggg
      721 ggttagatgg ccgcgcgaac ggtccgcgcc gccggtttag cggctgcccc tgggcgtcga
      781 cggaggggtc gaggatgatc atcgcgctgg cgacgtggtt ggtcggcggc gggttggtca
      841 gcgcggcgcg gatggcaata tcgagtgttg tcgcctcatc ttgcgcgccg ggcacgggat
      901 caaccatgcg gtggcaaacc tcgcaggcca ggccggcgtc cttatcctga tcggtcaggg
      961 cgctgccgtc ggccgggtgg ctgcggtcgg cgaaccagcc ggtgggcgca tggcagcgca
     1021 ggcagaactc gcccgcgtcg gtggcgtcgt cgttggccac ggcgaaagca gaccagaaca
     1081 gcgggtcgcg cccggcctgg cccatcatgc tgccgcgcca cgtctggtag atgggggcgg
     1141 tgtggcagct attgcagctc ctcgggtcag ctatctccgc gaacttcttg tcatcggggt
     1201 tgctgggctg cgtgccgggc aagaagaagt cgttgcgcgt cgtcggcaac ggcaggttgt
     1261 tgacgctggt cccggcggca ttgccggcgc tgtcgctggc gggcgaagcc tggcgggcgg
     1321 cggcgcggcc gacgccggcg gccagcatga ggatggccaa caatagcagg ccagccagga
     1381 tcgcgcggcc acgggacagg cgcggctgtg gggccgcggg tcgataattg gtgctcatgt
     1441 tgcctcactc tccttccatt ggcgctccag ccagaccagg gcagccaggg cgagcgcggc
     1501 agcgattccc cccaccaggt agccctcaac gacgctggcc tgggtgatgt acagcgccat
     1561 catacccaaa acgccgcaaa ataggtagag aatcagcact gcctcgcgcg gcgcgaagcc
     1621 cagccgcacc aggcgatggc tggtgtggtc gcgcccggcc gtgttggggc tgagtccccg
     1681 ccgcagccgc gagacgacga ccagggtcat gtcgaagata ggcagcccaa agaggaagac
     1741 gggcaccatc caggtgacga agttggaatt gtcgggaaag cgcaactgga tgcccatggc
     1801 cgccagcagg aagcccagaa acagcgagcc gacgtcgccc atgaaaatgc gcgccggcgg
     1861 gaagttgtag cgcaggaagc cgacgcaggc ccccagcaga gcggccgaca gcgccgccac
     1921 gagaaactgg ccattggtca gcgccagcaa aaggataaat gaagagatga cggcagcaag
     1981 gcccgcgctc aggccgtcca tgttgtcgag gaagttgatg gcgttggtga tgcccaacag
     2041 ccacagcagg gtaatggcgt agttgagcgc cgctggcagc ggcagccgga cgcgaatgcc
     2101 aaaggcggcc aggatcaggc agccggccag ttgaccggcg agcttggccc aggcgggcag
     2161 cggccggtag tcgtcgatca gcccggtcag ggccacgacc gtcgtcgcca ggagcacgcc
     2221 cagcaccgtc ggctctggtt cgccacgata aatgaccagc acagccagaa tcgcgcccac
     2281 gaagatgccc acgccgccga gcagaggcat gggcgtgcgc tgggtcttgc gctccgacgg
     2341 cgcatcgacc aggctaagcg ccagcgccgc ccgccgcacc cacggcagac cggcgagggt
     2401 gatggccagg gcgatgaaga aggcagcgag gaaggcgagc atgttgctga cccgggcggc
     2461 ttattggtcg agggtggcca ggaaggcctc gatctgggcg gccgtctcct cgccagctgt
     2521 ctccagggcg cgttcggcca gggcgcgggc ttcgtcgggc cggcccagtc cgtcgaggat
     2581 ctgcgcttcc aggaagtcca gttcggccgt cggaatggac acggggtcat ttaattctcg
     2641 ggcgtgggcc agggctgtcg cggcggcctc gtactcgcc
//
LOCUS       DAOSMQ010000448         2678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38131_length_2678_cov_4.491803, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000448 DAOSMQ010000000
VERSION     DAOSMQ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38131_length_2678_cov_4.491803"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..>2678
                     /locus_tag="PK829_12625"
     CDS             262..>2678
                     /locus_tag="PK829_12625"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOU42102.1"
                     /translation="MSLTRQYEALPAERAIEQQWQDSGVYHFRPDGDAPIYAIDTPPA
                     SVSGRLHLGHVYSYAHADFFARYRRMRGDNVFYPMGFDDNGLPTERLVERTRGVRASE
                     IGRAAFIRECLAVSAEVEAEYEALWRRLGLSVDWRYTYRTIDDDARRVAQWGFLDLLR
                     RDLAYQREAPTLWCPECRTAIAQAELNDLERETIFHTLAFGLAGGATLPIATTRPELL
                     PACVAVFVHPDDARFRPLIGQMATTPLFDRAVPILADPAADPALGTGAVMCCTFGDTA
                     DVAWQRRHDLPLIAAIGPDGRMTAAADPFAGLTTDAARRQLAAALDERGLLLDSRPIA
                     QTVRVHERCDTPVEYLTSRQWFIRILDQRAAFREAGERIAWHPEHMGLRYAQWVDNLS
                     WDWCISRQRAFGVPFPLWTCEACGAIILPNEDELPVDPTERPPSQPCACGSTAFRAET
                     DVMDTWATSSLTPQIAGRRLTDPALFQRVFPMALRPQAHEIIRTWAFYTIVRAHYHEG
                     ALPWTDVAISGWGLSPDGAKKLSKRHDDQPAGESETLAKSSAGGKPLGALAALDKYSA
                     DAVRYWAAGTGLGKDTIISEERIATGGKWATKLWNVARFAERFLDGYTPPAAPPAGLT
                     TADRWLLARQARLIAAATAAFDAYDYATARGEVETFFWRDLADNYIEMAKQRLYDGGA
                     GYEAARYTLYQALLTTVKLLAPLLPYITEAIYGGLFAASDGAASVHRAAWPAADAHFD
                     DEAALAAGETLLDIATAVRRFKNARNLSLGAPLAALHLPDTPAELAGPLRAATADLLS
                     ITRARALT"
BASE COUNT          404 a          996 c          906 g          372 t
ORIGIN      
        1 cgccgaaaga gaagacgggg ccacgggttg aaagcccccg cggtggaagc gcgtcgaagg
       61 tcgtccgggt tggttgccga agaaccgggc ctggcccgca gtagaccggc acgggtgcgc
      121 ccgttagcgc gccgccccga tggtggtcgg ggcatgagcg cgccggacgg ccggcgaact
      181 gaggtggtac cgcggaagca cagccctttc gtcctcatga tggacagagg gctgattgtg
      241 tttctatgga ggtatcacca gatgagtctg acacggcaat acgaagcgct ccccgccgag
      301 cgcgccatcg aacagcagtg gcaggacagc ggcgtctacc acttccggcc ggacggcgac
      361 gcgccgatct acgccatcga tacgccgccc gcgtcggtgt cgggccggct gcacctcggc
      421 cacgtctact cctacgccca cgccgacttc ttcgcccgct accggcgcat gcgcggcgac
      481 aacgtcttct acccgatggg cttcgacgac aacggcctgc cgaccgagcg gctggtggag
      541 cgcacgcgcg gcgtccgggc cagcgagatc ggccgcgccg cattcatccg cgaatgtctg
      601 gcggtgtcgg ccgaggtgga ggcggagtac gaggccctct ggcggcggct gggtctgtcg
      661 gtcgattggc gctacaccta ccgcaccatc gacgacgacg cgcgccgcgt cgcccaatgg
      721 ggcttcctcg acctgctgcg gcgcgacctg gcctaccagc gcgaagcgcc gacgctgtgg
      781 tgcccggagt gccgcacggc cattgcccag gcggagctga acgacctgga gcgcgagaca
      841 atcttccaca cgctggcctt tggtctggcc ggcggggcga cgctgcccat cgctacgacc
      901 cggccggaac tgctgcccgc ctgcgtggcc gtcttcgtcc accccgacga cgcccgcttc
      961 cggccgctca tcggccagat ggccacgacg ccgctcttcg accgggccgt gcccatcctg
     1021 gccgacccgg ccgcggaccc ggcgctgggc acgggcgcgg tgatgtgctg caccttcggc
     1081 gacacggccg acgtggcctg gcagcgccgc cacgacctgc cacttatcgc cgccatcggc
     1141 ccggacgggc gcatgaccgc cgcggccgac cccttcgccg ggctgacgac cgacgcggcc
     1201 cgccgccaac tggccgcggc gctggacgag cgcggactgc tgctggacag ccggcccatc
     1261 gcccagaccg tgcgcgtcca cgagcgctgc gacacgccgg tggagtacct caccagccgg
     1321 caatggttca tccgcatcct cgatcagcgc gccgccttcc gcgaggccgg cgagcgcatc
     1381 gcctggcacc cggagcacat gggcctacgc tatgcgcaat gggtggacaa tctcagctgg
     1441 gactggtgca tctcgcggca gcgggccttt ggcgtgccct tcccgctgtg gacctgcgag
     1501 gcgtgcggag ccatcatcct gcccaatgag gacgagttgc ccgtcgaccc aactgagcgc
     1561 ccgccgtcgc agccctgtgc ctgcggcagc acggccttcc gggcggagac ggacgtgatg
     1621 gacacttggg cgacctcatc gctgacgccg cagatcgccg gtcgccgcct gaccgacccg
     1681 gccctcttcc agcgcgtctt cccgatggcc ttgcggccgc aggcgcacga gatcatccgc
     1741 acctgggcct tctacaccat cgtgcgtgcc cactaccacg agggcgcgtt gccgtggacg
     1801 gacgtggcca tctccggctg gggcctgtcg cccgatggcg caaagaagtt gagcaagaga
     1861 cacgacgacc agccggcagg agagtcagag acgttggcca aatcctcggc cggcggcaag
     1921 ccgctagggg cgctagcggc gctggacaag tactcggccg acgccgtgcg ctactgggcc
     1981 gcgggcacgg ggctgggcaa ggacaccatc atcagcgagg agcgcatcgc caccggcggc
     2041 aagtgggcca ccaagctctg gaacgtggcc cgcttcgctg agcgcttcct cgacggctac
     2101 accccgccgg ccgcgccgcc agcgggcctg accacggccg accgctggct gctggcccgc
     2161 caggcccgtc tgatcgccgc cgccacggcc gccttcgacg cctacgacta tgctaccgcc
     2221 cgcggcgagg tggagacgtt tttctggcgc gatctggccg acaactatat cgagatggcc
     2281 aagcagcggc tctacgacgg cggcgcgggc tacgaagcgg cgcgctacac gctctaccag
     2341 gccctgctga caaccgtaaa gctgctggct ccgctgctac cctacatcac cgaggccatc
     2401 tacggcggcc tgttcgcggc gagcgacggc gcggcctccg tccatcgggc cgcgtggccg
     2461 gcggccgacg cacacttcga cgacgaagcc gccctggccg cgggcgagac gctgctcgac
     2521 atcgccacgg ccgtgcgccg cttcaagaac gcgcgcaacc tgtcactggg cgcgccgctg
     2581 gcggcgctgc acctgcccga cacgccggcc gagttggccg ggccgctccg cgcggcgacg
     2641 gccgacctgc tcagcatcac ccgcgcccgc gccctgac
//
LOCUS       DAOSMQ010000449         2677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38147_length_2677_cov_4.131579, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000449 DAOSMQ010000000
VERSION     DAOSMQ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38147_length_2677_cov_4.131579"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1284
                     /locus_tag="PK829_12630"
     CDS             19..1284
                     /locus_tag="PK829_12630"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOU42103.1"
                     /translation="MHNSSTAPAMSTSMRLIALLLVPLLLLPLLLGRPADAGSAAAAS
                     AAASRVFLPLALDRATFRASPLGSGFSQVTEITDAGDDRLFIAERAGIVKILHPDGRI
                     TTFLDIRQRVISNDGSEYGFFDIAFHPGYKDPTSPGYGFFYVSYTTGKDDGVIIDVHF
                     NVSRFRVSADPDVADPNSEVLLQHEKQSFNVHKGGGMEFDPRDDMLYVGMGDDRLLLI
                     AQSDRSPKGKISRLPVDNVPRDATPRSLATISEEIWVFGLRNPWRFDLDPATNSIFIG
                     EVGDYLWEEVNLAPMTARGYNFGWPCMEGGFVFPETNEVPQCQTPWLFKRAIYEYPHN
                     DGSGRCAVIGGHVNRPVYNPTDGRYIFGDMCSREIYSLSRNAAGAWQRTLLGVVPGDL
                     INTIGEDRHGTQYVGTAARGGPIWKLFIP"
     gene            1317..2558
                     /locus_tag="PK829_12635"
     CDS             1317..2558
                     /locus_tag="PK829_12635"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOU42104.1"
                     /translation="MRGYTKFTTACMAGLLLLLASLTLRPATARPAASGRVFVPLAAG
                     KVAFGATAIPGTYNEVTAITHAGDDRLFIGEREGRVKILHPDGRVTVFLDIRHRVIAS
                     RGEYGFYDIAFHPGYADPASPGYGFFYVSFTSGTDERAAGGAAAVDVDLIVARFRVSA
                     DPNVANPASEVPLLVVPQTDDIHKGGAMEIDPRTDRLFVGVGDDFKNLIAQDPGSPKG
                     KILRLAVDEAPRDAPPYQLYPVTTEVWASGLRNPWRIDVDPLGDQLLIGEVGDADWEE
                     VNRAPLSAPGANFGWPCLEGPLVKPSFSGAAACQNPERFERPVHAYSHRDTDNNRCVI
                     IGGKVYRPAGNPTDGRYIFADLCSREVFALGYDADGSWPRTLLGVVGNELINTIGEDR
                     YGTQYIGTAARGGVIWRLSIP"
BASE COUNT          441 a          973 c          822 g          441 t
ORIGIN      
        1 cctcaacgcg agggaaccat gcacaactca tctactgccc cggccatgtc cacgtccatg
       61 cgcctgatag cgctgctcct tgtccctcta ctcctcttgc ccctgctgct cggccgcccc
      121 gctgacgccg gcagtgccgc ggccgccagt gcggccgcca gccgcgtttt ccttcccctg
      181 gccctcgacc gggccacttt ccgcgcctcg ccgctcggca gcggcttcag ccaggtaacg
      241 gagatcaccg acgccggcga cgaccggctg ttcattgccg agcgcgccgg catcgtcaag
      301 atcctgcacc ccgacggccg catcactacc tttctcgaca tccgccagcg ggtcatcagc
      361 aatgacggct ccgagtatgg cttcttcgac atcgccttcc accccggcta caaggacccc
      421 acctcgcccg gctacggctt cttctacgtc agctacacaa cgggcaaaga cgacggtgtg
      481 atcatcgacg tgcatttcaa cgtctcgcgc ttccgcgtct cggccgaccc ggacgtggcc
      541 gaccccaaca gcgaagtgct cttgcagcac gagaagcaat cgttcaacgt ccacaagggc
      601 ggcggcatgg agttcgaccc gcgcgacgac atgctctacg tcggcatggg cgacgaccgg
      661 ctgctgctca ttgcccagag cgaccggtcg cccaagggca agatctcccg tctgcctgtg
      721 gacaatgtgc cgcgcgacgc caccccgcgc agcctggcca ccatcagcga agaaatctgg
      781 gtcttcggcc tgcgcaaccc gtggcgcttc gacctcgacc cggcgaccaa ttcgatcttc
      841 atcggcgaag tgggcgatta tctgtgggag gaagtcaacc tggcccccat gaccgcccgc
      901 ggctacaact tcggctggcc atgcatggaa ggcggcttcg tctttccgga gacgaacgag
      961 gtaccgcaat gccagacgcc ctggctgttc aagcgggcta tttacgagta cccccataac
     1021 gacggcagcg gccgttgcgc cgtcatcggt ggccatgtca accggccggt ctacaacccc
     1081 actgacggtc gctacatttt cggcgacatg tgcagccgtg agatctacag tctgtcgcgc
     1141 aacgccgccg gagcctggca acgaacgctg ctgggcgtcg tccccggcga tctgatcaat
     1201 accatcggcg aagaccgcca cggcacacag tacgtcggca cggccgcgcg cggcgggccg
     1261 atctggaagc tgttcatccc ctagccgccg tggccgcgcc ggggacgaag gttaagatga
     1321 ggggctacac gaaattcact acggcttgca tggccggtct gctactcctg ctggccagcc
     1381 tgaccctccg gcccgccacc gcccgcccgg ccgcgtctgg gcgcgtcttt gtgccattgg
     1441 ccgcgggtaa ggttgccttc ggcgcaacgg ccataccggg aacgtataac gaggtcacgg
     1501 ccatcaccca cgccggtgac gaccgcctgt tcatcggtga gcgcgagggc cgggtgaaga
     1561 ttctccaccc cgacggccgc gtgaccgtct tcctcgacat ccgccatcgg gtcatcgcca
     1621 gccgcggcga gtacggcttc tacgatatcg cctttcaccc cggctacgcc gacccggctt
     1681 cgcccggcta tggcttcttc tatgtcagct ttaccagcgg cacggacgag cgggcggccg
     1741 gcggcgcggc ggcggttgat gtcgacttga tcgtggcccg cttccgcgtc tcggccgacc
     1801 cgaacgtggc caacccggcc agcgaagtcc ccttgctggt cgtgccccag accgacgaca
     1861 tccacaaggg cggcgcgatg gagatcgacc cgcgcaccga ccgcctcttc gtgggcgtgg
     1921 gcgacgactt caagaacctc attgcccagg accccggctc gcccaagggc aaaatcctgc
     1981 ggctggcggt tgacgaagcg ccgcgcgacg cgccgcccta tcagctttat ccggtgacga
     2041 ccgaggtctg ggccagcggg ctgcgcaacc cgtggcggat cgacgtggat ccgctgggcg
     2101 atcagctgct catcggcgag gtgggcgacg ccgattggga ggaggtcaac cgcgccccgc
     2161 tgagcgcgcc gggggccaac ttcggctggc cgtgtctgga ggggccgctg gtgaagccta
     2221 gtttttccgg cgcggcggcc tgccagaacc cggagcggtt tgagcggccg gttcacgcct
     2281 actcccatcg cgacacggac aacaaccgtt gcgtgatcat cggcggcaag gtctaccgcc
     2341 cggccggcaa cccgaccgat ggccgctaca tcttcgccga tctgtgcagc cgcgaggtct
     2401 ttgccctggg ctacgacgcc gacggctcgt ggccgcgcac gctgctgggc gtcgtcggca
     2461 atgagctaat caacaccatc ggcgaagacc gctatggcac acagtacatc ggcacggccg
     2521 cgcgcggcgg ggtgatctgg cggctgtcca ttccctaggg gttcagcgcg gcgcgccccg
     2581 ctggccggcc catccgtcac cgaaggtagg cggccgcgcc cctagaaccg cgcccgcagc
     2641 cgcgcccgtc cgccccagcc cgacgccagg aacaaca
//
LOCUS       DAOSMQ010000450         2674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38205_length_2674_cov_3.567010, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000450 DAOSMQ010000000
VERSION     DAOSMQ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38205_length_2674_cov_3.567010"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1504)
                     /locus_tag="PK829_12640"
     CDS             complement(287..1504)
                     /locus_tag="PK829_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOU42105.1"
                     /translation="MPTGTGPTTTSSDDGGRGHVIGIDATAALTQGGGIGRYTRELIH
                     ALVAEAPDNHYTLFSARPPATLLVPDSLPVAAHVVHRPAPLDERWLYRLWYRARIPLP
                     VQTFTGPLDLFHSPDFVLPPVGGGIPTLLTVHDLSFVHYPDTFPAQLVAYLSRVVPRS
                     VARADHILADSEATQRDLAALWNVPANKVTVLYSGVNARFRPVTDPARLAAVRARYGL
                     GERPIVLAVGTVQPRKNYALLVRAFRAVAAAQPHQLVIVGGRGWLTEGLEAEIARQGL
                     GQRVVMPGFVDDADLPALYSAADVFVFPSLYEGFGLPLLEAMACGVPVISSDASSLPE
                     VAATGGEPAALLLSPQDEAGWSAAMLRLLAEPAARQRLIAAGAAQAARFTWPAAARQL
                     AGLYDRLLARGER"
     gene            complement(1467..2030)
                     /locus_tag="PK829_12645"
     CDS             complement(1467..2030)
                     /locus_tag="PK829_12645"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU42106.1"
                     /translation="MSRMITTLEHVSTRLGVKPREAKRFVKFLIVGAIGFIVDFGFFN
                     LLLGPINHWVLPGQWIYNLLTRMGFGAGFIAHLGPSIASTISFVAAVCSNYYWNRHWT
                     YPDSRSRSPRRQFTMFFLVSVVGVLIRIPIVFVMTPLFRSMVSGIGPLQAYTTRLADN
                     LSLMVAVVVVLFWNFFANRHWTYNDVK"
     gene            complement(2033..>2674)
                     /locus_tag="PK829_12650"
     CDS             complement(2033..>2674)
                     /locus_tag="PK829_12650"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU42107.1"
                     /translation="IFLVAGISLAAVMLGVAADFGPALSVADRLGGFAGQFTLGDMRG
                     VDITTANFAVLERLAHWQAAVDMARDNLWMGVGFGNYEAAYPAYALINWPDALGHAHN
                     YYLTLLAEVGLPGLLAYVVFWLAVAWQTARTARQLAWPKRGVVIGLLAAWAALAVHHL
                     VDKLYVNNIYVHLGAMLAVLQLLAAPTWKGATANRTGNPPVASSAGLPPDEIA"
BASE COUNT          546 a          848 c          897 g          383 t
ORIGIN      
        1 ccgtcgccag ccaggccgcc caccaagaca gtctaccgtc ctggcagcca cagggcgcgg
       61 cccattgcgg cacaggcgct cgatctcaat tgtaggtact actcgttcgt tacatggtcg
      121 gcccggggtt cttcaggccg ataaggacat tataggtagc cagtcctggc cgcgcaagtg
      181 atctggggct tttcacaggg ctttcagccg tcgtcagctg gctaaagacc ggacaggcga
      241 acgacccaaa tctcctgaaa tgacaggcac cgcccgtaac caggcatcaa cgctcgccgc
      301 gggccagcag ccggtcatac aggccggcca gttgccgcgc cgcggccggc caggtgaagc
      361 gcgctgcctg ggccgccccg gcggcgatca ggcgctgccg ggcggccggc tcggccagca
      421 ggcgcagcat ggccgctgac cagccggcct cgtcctgtgg cgacaggagc agcgccgccg
      481 gctcgccgcc cgtcgcggcg acctccggaa ggctggaggc gtcggagctg atgacgggca
      541 cgccacaggc catcgcctcc aacaagggca agccaaagcc ctcatagagg ctgggaaaga
      601 cgaacacatc cgcggccgaa tacagcgccg gcaggtcggc gtcatcgacg aagccgggca
      661 tgacgacgcg ctggcccagc ccctggcggg cgatctccgc ttccagtccc tctgtcagcc
      721 agccccgccc gccaacgatg accagttggt ggggctgggc cgcggccaca gcccggaagg
      781 cgcgcacgag cagggcatag ttcttgcgcg gctgcaccgt gcccacggcc aacacaatcg
      841 gccgctctcc caacccatag cgcgcccgta ccgcggccag gcgcgccgga tcggtcaccg
      901 ggcggaagcg cgcgttcacg ccgctgtaga gcaccgtcac cttattggcg ggcacgttcc
      961 acagggcggc caggtcgcgc tgcgtggctt cggaatcggc caggatgtgg tcggcgcggg
     1021 ccaccgaacg aggcacgacg cggctgagat aggccaccag ttgggccggg aaggtgtcgg
     1081 gatagtggac gaacgacagg tcatggacgg tcagcaacgt ggggatgccg ccgccaacgg
     1141 gtggcaggac gaagtcgggc gagtggaaca gatcgagcgg gccggtaaag gtctgcactg
     1201 gcaaggggat gcgggcgcga taccacagcc ggtagagcca gcgctcgtcg aggggcgcgg
     1261 gtcggtggac aacgtgagca gcgacaggca gcgaatcggg cacgagcagc gtcgccgggg
     1321 gtcgggccga aaaaagcgtg tagtgattgt ctggcgcttc ggctaccagg gcgtggatga
     1381 gttcgcgtgt gtagcggccg atgccgccgc cctgggtcag cgccgcggtg gcgtcaatgc
     1441 cgatgacgtg cccccgcccc ccgtcgtcac ttgacgtcgt tgtaggtcca gtgccggttg
     1501 gcaaagaagt tccagaaaag aacaacgacg acggcaacca tcagcgacag gttatcggcc
     1561 aggcgggtcg tataggcttg cagcgggccg ataccgctca ccatcgaccg gaacagcggc
     1621 gtcatgacaa agacaatggg gatacggatg agcacgccga cgacgctgac cagaaaaaac
     1681 atcgtaaatt gccgccgcgg cgaacgggag cgcgagtcgg ggtaagtcca gtggcggttc
     1741 cagtagtagt tgctgcacac cgcggccacg aaggagatgg tcgaggcaat ggacgggccg
     1801 aggtgggcga taaagccagc gccaaagccc atgcgcgtca agaggttata gatccactgg
     1861 ccgggcaaca cccagtggtt gattgggcca agcaacagat tgaagaagcc gaagtcgaca
     1921 atgaagccga tggctcccac aatgaggaat ttgacaaagc gcttggcttc tctgggctta
     1981 acgcccaagc gggtcgatac atgttccagc gtcgtgatca tccttgacat ctctaggcaa
     2041 tctcatccgg gggtaagccg gctgaggacg caacaggtgg attaccggta cgattggccg
     2101 ttgcgccctt ccaagtaggc gcagccagca gttgtaacac ggccagcatc gcgcccaaat
     2161 gaacgtaaat gttattcaca tatagtttat ccacgagatg gtggactgcc aatgccgccc
     2221 aggccgccaa caaccctatg accaccccgc gcttgggcca cgccaactgc cgcgccgtcc
     2281 gcgctgtctg ccaggcgacg gccagccaga acacaacgta ggccagcaga cccggcagac
     2341 ccacctcggc cagcagggta aggtagtaat tgtgggcgtg gcccagggcg tcgggccagt
     2401 tgatgagcgc gtaggcgggg taggcggcct cgtagttgcc gaagcccacg cccatccaca
     2461 ggttgtcgcg ggccatgtcg acggccgcct gccagtgggc cagccgttcc aacacagcga
     2521 agttcgcggt ggtgatgtcc acgccgcgca tgtcacccag cgtgaactgg ccggcgaagc
     2581 cacccaggcg gtcggcgacg gatagcgccg gcccgaagtc cgcggccacg cccagcatca
     2641 ctgcagccag actgatcccc gcaacaagaa agat
//
LOCUS       DAOSMQ010000451         2670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38264_length_2670_cov_4.684130, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000451 DAOSMQ010000000
VERSION     DAOSMQ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38264_length_2670_cov_4.684130"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="galT"
                     /locus_tag="PK829_12655"
     CDS             complement(<1..714)
                     /gene="galT"
                     /locus_tag="PK829_12655"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOU42108.1"
                     /translation="MDAEALFAAPHRRQNLLTGEWVLVSPQRTARPWRGQMEAPPAGA
                     RLAHDPDCYLCPGNARAGGNVNPDYAATFVFDNDFAALRPDTAPGELRRGLTDAGPPL
                     LRAEAERGICRVVCFSPRHDLTLADMPLAGLRRVVDTWAEQTAELGARPWVRHVQVFE
                     NRGALMGASNAHPHGQIWADERLPNEPAQELVEQRAFAAASGGCLLCDYLALELRLGE
                     RVVCANDHWVALVPFWAVWP"
     gene            899..>2670
                     /locus_tag="PK829_12660"
     CDS             899..>2670
                     /locus_tag="PK829_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU42109.1"
                     /translation="MLLFGLPAFRRAEYWSIPMADREQLKQAIAVQESLRGQIDDAIL
                     DATLAALRRQLAELDAPPPRRAQATLLFLDLAGHTELIQGLDPEEIVETIDRALARLA
                     APVRRRGGRIVRFQGDGFKAVFGLPTAHEKDPDHAIRAALDILSETQAIAAELEAERG
                     RPGFMARIGIDTGLVLIGEGAEGEDSVTGLPVNLAARLEGLAEPGTVLISHHTYQHVR
                     GVFDMQALPPVQVRGFAQPVPTYRILRPKARSFRTRRRGVEGIETRMIGRDAEMSRLQ
                     AIYTALVEDGRGRVITVVGEAGLGKSRLLYEFENWGDLQPVNVQLYRGRARQETQQLP
                     FGLLRDVFVFRCGIFDDDEPAAVRDKLVAGFRALLGDDTAELSAHLVGHLLGYDFSAS
                     PHVRALQGDARQLRDLALAQMTAYFRAATGRRPLLLLLEDLHWADDSSLDTIEALAAA
                     LRDHPLVILGAARPALYERRPGWMANQPQHERIDLALLGDDSSRQLVTEILQKADAIP
                     DRLRDLVVARAEGNPFYVEELIKMLIEQGVIERRDLAEATGQASEPPAPDEDEHWLIH
                     PERLDAIHIPGTLTGVLQARLDSLP"
BASE COUNT          420 a          947 c          905 g          398 t
ORIGIN      
        1 gggccacacc gcccagaagg ggaccagcgc tacccagtga tcgttggcgc agaccactcg
       61 ctcgcccagg cgcaactcca acgccagata gtcgcacagc aggcagccgc ccgacgccgc
      121 ggcaaaggcg cgttgctcga caagctcctg ggccggctcg ttgggcagcc gttcgtcggc
      181 ccatatctgg ccgtgggggt gggcgttgct ggcccccatg agcgcgccgc ggttctcgaa
      241 gacctgcacg tggcgcaccc agggacgcgc ccccaactcg gccgtctgct cggcccaggt
      301 atcgacaacg cggcgcaggc cggccagggg catatcggcc agcgtcaaat cgtggcgggg
      361 cgagaagcag accacgcggc agatgccccg ctcggcctcg gcgcgcagca ggggcgggcc
      421 ggcgtcggtc aggccgcggc gcagctctcc cggcgcggtg tctggccgca gcgcggcgaa
      481 atcgttgtcg aagacgaacg tggccgcgta atcggggttg acgttgccac cggcgcgggc
      541 gttgccgggg cacagatagc aatcggggtc gtgggccagg cgcgccccgg ccgggggcgc
      601 ctccatctgc ccgcgccacg gccgggccgt gcgctgcggc gagacgagca cccattcacc
      661 cgtcagcagg ttctggcggc ggtggggggc agcaaagagt gcttcggcat ccattgttgc
      721 tgttctgctc ctgtgatcgc gccgcggcct cagagctccc caggcgcacc gacctgaccg
      781 gtgggcggcc acgaagggaa ctgcccccag agaacgactg gccgcccacc ggtcaggtca
      841 atggtcgcta gacggctttg gaaaagccct ggccgcggcc tgcccacgct ccataactat
      901 gctattattc ggcctgcctg cgttccggcg ggcggagtac tggagtatcc ctatggccga
      961 ccgcgagcaa ctcaagcaag ccattgccgt gcaggagagc ctgcgcggcc agatcgacga
     1021 cgctatcctg gatgctaccc ttgccgccct gcgccgtcaa ctggccgaac tggacgcgcc
     1081 gccgccgcgc cgcgcccagg caacgctgct gtttctcgac ctggccggcc acaccgaact
     1141 gatccagggc ctggacccgg aggagatcgt cgaaacgatc gaccgggcgc tggcgcggct
     1201 ggccgcgccg gtacgacgac gcggcggccg gatcgtccgc ttccagggcg atggcttcaa
     1261 ggccgtcttt gggctgccga cggcccacga gaaggaccct gaccatgcca tccgcgccgc
     1321 gctggacatt ctgagcgaga cgcaggccat tgccgccgaa ctggaagccg agcgcggacg
     1381 gcccggtttc atggcccgca tcggcatcga caccggtctg gtgctcattg gcgaaggggc
     1441 cgagggcgag gactccgtca ccggcctgcc ggtcaacctg gccgcgcggc tggaggggct
     1501 ggccgagccg ggcacggtgc tcatctccca tcacacctac cagcatgtgc gcggcgtctt
     1561 tgatatgcag gcgttgccgc cggtgcaggt gcgcggcttc gcccagcctg tgccgaccta
     1621 tcgcatcctg cggccaaagg cgcgctcctt ccgcacgcgg cggcgcgggg tggagggcat
     1681 cgagacgcgc atgatcggcc gcgacgccga gatgagccgc ctgcaagcga tctacaccgc
     1741 ccttgtcgag gacgggcgcg ggcgtgtcat caccgtcgtc ggcgaggccg ggctgggcaa
     1801 gtcgcgcctg ctgtacgagt tcgagaactg gggcgacttg cagccggtca acgtccagct
     1861 ttaccgcgga cgtgctcgcc aggagacgca acaactgccc tttggtctgc tgcgcgatgt
     1921 cttcgtcttt cgttgcggca tcttcgacga cgacgagccg gcggctgtgc gcgacaagct
     1981 ggtggcgggc ttccgcgccc tgctgggcga cgacacggcc gagctgtcgg cccatttggt
     2041 cggtcacctg cttggctacg acttcagcgc cagtccccac gtgcgcgcct tgcaaggcga
     2101 cgcgcgtcaa ctgcgcgacc tggccctggc gcagatgacg gcctacttcc gcgccgccac
     2161 gggccgccgg ccgctgctcc tgctgctaga agacctccac tgggccgacg atagctcact
     2221 cgataccatc gaagcgctgg ccgccgcctt gcgcgaccat ccgctggtca tcctcggcgc
     2281 ggcccggccg gcgctctacg aacgccgccc cggctggatg gccaaccagc cacagcatga
     2341 gcgcatcgac ctggccttgc tgggcgacga cagcagccgc caactggtga ccgaaatcct
     2401 ccagaaggcc gacgccatcc ccgaccgcct gcgcgacctg gtggtggctc gcgccgaggg
     2461 caatcccttt tacgtcgaag agttgatcaa gatgctcatc gagcagggcg tcatcgagcg
     2521 gcgcgacctg gccgaggcca ccggacaagc cagcgaaccg cctgcgccgg atgaggatga
     2581 gcactggctc atccacccgg aacggttgga cgccattcac atacccggca cactgaccgg
     2641 cgttttgcag gcccgcctcg acagcctgcc
//
LOCUS       DAOSMQ010000452         2669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38283_length_2669_cov_3.714996, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000452 DAOSMQ010000000
VERSION     DAOSMQ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38283_length_2669_cov_3.714996"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PK829_12665"
     CDS             complement(<1..1108)
                     /locus_tag="PK829_12665"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOU42110.1"
                     /translation="MLTIDLDVARRFILGKGGLWPGRRWRGVEGVAAAMRAMDYLQLD
                     PLQIVARSQDIQLHSRVLDTTPGAWEEVTYGQRRFFDWGGWLAVRPMDELPHWRVAMR
                     RERDGDPPNVDTRIRAMGREHADAVVEMRAILRQRGTVSNRDFAAASRTRTQSYRGRK
                     DSSLALYYLWRTGEVMTHHRENFERVYALTETVAPPDLIRESDDADADRFLVLKDISF
                     AGLSRLNRTADSWQRVVPFSRTDRIIAALLDRGEIVEVRVEGWKGTSFARGEDAETLL
                     DLAAGRVPPAWTPLETDTTAEVVFLAPLDPVSARGRAKQLFGFEYIWEVYKPADKRKH
                     GYYTLPVLWGERLVARFDSKLDRTTNTFVVLGFWL"
     assembly_gap    1419..1518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1673..>2669
                     /locus_tag="PK829_12670"
     CDS             1673..>2669
                     /locus_tag="PK829_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU42111.1"
                     /translation="MIQRDDIRNIAIIAHVDHGKTTLVDGMLKQTHAFRQNQQVAERV
                     LDSNDLERERGITILAKNTGIDYKGVTINIVDTPGHADFGGEVERIMNMVDGALLLVD
                     AVEGPMAQTRFVLRQALAKGLKVILVVNKIDRPAARPDYAVNATFDLFIDLGATEEQA
                     HFPVIYAKATDGRAGLSPAELDSDLTVLFDTILEHIPPPTVDPDGPTQLLVTALEYSS
                     YVGKIAVGRLTAGRLRAGQTVAHIDHHGNMATGKVTKLYTFRDLQRQEQSEVSAGTIV
                     AVAGIPGLGIGDTLADADNPVALPPIKVEEPTVRMVFRINDSPFAGREGKYVTSRQ"
BASE COUNT          448 a          840 c          846 g          435 t
ORIGIN      
        1 ccagccagaa gccgaggacg acaaaggtgt tggtcgtccg gtcgagctta ctatcgaagc
       61 gagccacaag acgttcgccc cacaggacgg gcagggtgta gtagccgtgc ttgcgcttgt
      121 cggccggttt gtagacctcc cagatgtact caaagccgaa tagctgcttg gcccggccgc
      181 gagcgctaac agggtcgagc ggggccagga agaccacctc ggccgtcgtg tccgtctcca
      241 gcggtgtcca cgccggcggc acgcggccgg cggccaggtc gagcagggtt tcggcgtcct
      301 cgccacgggc gaagctcgtc cccttccagc cctcgacgcg cacctcgacg atttcgccac
      361 ggtccagcag cgcggcgatg atgcggtccg tgcggctgaa gggcacgacg cgctgccagg
      421 aatcggccgt gcggttcagg cgcgacaggc cggcgaagct gatgtccttg agcaccagga
      481 agcggtcggc gtcggcgtcg tcgctctcgc ggatgaggtc gggcggggcg acggtctcgg
      541 tcagggcata gacgcgctcg aagttctcgc ggtggtgggt catcacttcg ccggtgcgcc
      601 agagatagta gagggccagg gagctgtcct tgcggccgcg gtagctctgg gtgcgcgtgc
      661 gcgaggcggc agcgaagtcg cgattgctga ccgtgccgcg ctggcgtagg atggcgcgca
      721 tctccaccac ggcgtcggcg tgttcgcggc ccatcgcccg aatgcgcgta tcgacgttgg
      781 gcgggtcgcc gtcgcgctcg cggcgcatgg ccacgcgcca gtggggcagt tcgtccatcg
      841 gccggacggc cagccagccg ccccaatcga agaaacggcg ctggccatag gtcacttcct
      901 cccacgcgcc gggggtggtg tccagcacgc ggctgtggag ttggatgtcc tggctgcggg
      961 cgacgatttg cagggggtcg agttgcaggt agtccatggc ccgcatagcc gcggcgacgc
     1021 cttccacgcc gcgccagcgc cggccgggcc acaggccacc cttgccgagg atgaagcggc
     1081 gggccacgtc gaggtcaatt gtcagcatgg cggtcagacc tccttctgaa atggcgcgag
     1141 cgggcattct gtgtcgttgt cgcccgcccg ggcgccagca ggctggtaaa atgtgcccgg
     1201 cagtatagaa ccttcgttct atccttgcaa gctcaatgga cggctctttt ttctttgcgc
     1261 ctttgtccct tcgcgccctg gcgcgctctt gttgtccgct tgcccatccc tctctctccc
     1321 gctataatct ttagtcggct accgacctga acaagacaca cccgcccggc ccacccgccg
     1381 ggcttttttg gcgcagtaag acctcacgca aagatcgcnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnngc ccggcccacc cgccgggctt ttttggcgca gtaagacctc
     1561 acgcaaagat cgccaaggac gccaaggacg ccaagataga aaagagcttt gcgctcttag
     1621 cgagcttgac gtgaggttct tggcgtgagg ttcttaagcc acggatagga ccatgatcca
     1681 gagagacgac attcgcaata tcgctataat cgcccacgtc gaccacggca agacgacgct
     1741 ggtcgatggc atgctcaaac aaacccacgc ttttcggcag aaccagcagg tggccgagcg
     1801 cgtgctagac agcaacgacc tggagcgcga gcgcggcatc accatcctgg ccaagaacac
     1861 cggcatcgac tataagggcg tgacgatcaa catcgtcgat acgcccggcc acgccgactt
     1921 tggcggcgag gtggagcgca tcatgaatat ggtcgatggc gcgctgctgc tggtcgatgc
     1981 cgtcgagggg ccgatggcgc agacgcgctt tgtgctgcgg caggcgctgg ccaaggggct
     2041 gaaggtcatc ctggtggtca ataagatcga ccgcccggcg gcccggcccg actacgccgt
     2101 caacgccacc ttcgacctgt tcatcgacct gggcgcgacg gaggagcagg cccactttcc
     2161 ggtcatctat gccaaggcga ctgacggccg cgccggcctc tcgccggccg agcttgactc
     2221 cgacctgact gtgttgttcg acaccatcct cgagcacatc ccgccgccga cggttgaccc
     2281 cgacgggccg acgcaactac tggtgacggc actggagtac agcagctacg tgggcaagat
     2341 cgccgtcggc cgtctgacgg ccgggcggct gcgggccggg cagacggtgg cccacatcga
     2401 ccaccacggc aacatggcca cgggcaaggt caccaagctc tacaccttcc gcgatttgca
     2461 gcggcaggaa cagagcgagg tcagcgctgg cacgattgtc gccgtggccg gcatccccgg
     2521 tctgggcatt ggcgacacgc tggccgacgc cgataacccc gtggctctgc cgcccatcaa
     2581 ggtggaagag ccaacggtgc gcatggtctt ccgtatcaac gacagcccct tcgccgggcg
     2641 cgagggcaag tacgtcacca gccgccagt
//
LOCUS       DAOSMQ010000453         2662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38421_length_2662_cov_4.381281, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000453 DAOSMQ010000000
VERSION     DAOSMQ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38421_length_2662_cov_4.381281"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK829_12675"
     CDS             complement(<1..599)
                     /locus_tag="PK829_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOU42112.1"
                     /translation="MRVKVILNPRSGQGRAREQTGMIDTLGQRYGDLDIVPTQRPGHA
                     VELAAAAASEGYDLVAAAGGDGTVHEVICGLMGDGDRATLPLGIIPIGSGNDLAYGLG
                     IREKPEEAVQRVFTGTRRAIDLGRVVDDRGRATVFHNNLGIGFDAIVVMRTETISRIQ
                     GFLMYLTAVLQTIAFYYETPYMEMTFDGQPVNQRALFLYA"
     gene            746..2593
                     /locus_tag="PK829_12680"
     CDS             746..2593
                     /locus_tag="PK829_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42113.1"
                     /translation="MSADDSVVNNGEVDNTNAGAPVADGNRTFLVLAALLTGTLLLFS
                     LFTVGYLLLGRAGGRSAEIAAIETQNAVILATNAAVTETIAAMNVEATSRAGASVAAV
                     EDTATPMAAATAEEDNAAPAATSEGPAVDSPESMTATIETLAANPTLAAAATSAAAGE
                     PGPTATPPATGEEGTPAANTTASPDETAESATTPVDATAASGETSAAGQDTPSATAAT
                     AEPGEATSVGTDAATSVPTDATSTAGDATAATADATANATAATANANATTTPLPTSAA
                     GAGTTATATSSVLPVPTATEAGRVPAVGATATPASTVVLLGVTQEATGLPTSSTTEAE
                     ATTVVGQPGGSAPVATGTPTPSPFGGGGDESGGGVPETPAATALPADAGYPPAATEPA
                     ADYPAPATSTPVSNVPDQPTAGAATPTQVGGATPNLPGSPTPTLAPTNTPTLVASNTP
                     TLVPSSTPTLVASSTPTLVPTSTATTVATTTATMVATTTATTTATTTATVSASPTATT
                     TATALPAVSPTTTATATATALPGTATPTPTPDPNATATATQTATVAPTVTSTAAATAT
                     GTVMAPTPTATPTPPVTLPETGLDAWGIAAVGLALALVVFGARRLRRAG"
BASE COUNT          466 a         1005 c          852 g          339 t
ORIGIN      
        1 gcatagagga agagcgcccg ctgattgacc ggctggccgt cgaaggtcat ctccatgtag
       61 ggcgtctcat agtagaaggc gatggtttgc agcacagccg tcaggtacat caggaagccc
      121 tggatgcggc tgatcgtctc cgtgcgcatg acgacgatgg cgtcgaagcc gatgccgagg
      181 ttgttgtgga agacggtagc gcgcccgcgg tcgtcaacta cccggccgag gtcgattgcc
      241 cgccgcgtgc ccgtgaaaac ccgctgtacg gcctcctccg gcttctcgcg gatgcccagc
      301 ccataggcca ggtcattgcc cgagccaatg gggatgatgc ccagcggcaa ggtggcgcgg
      361 tcgccgtcgc ccatcagccc gcagatgacc tcatgaaccg tgccgtcgcc gccagccgcg
      421 gccaccaggt catacccttc gctggcggcg gccgcggcca gttccacggc atggccaggc
      481 cgctgggtcg gcacgatgtc gaggtcgccg tagcgctgcc ccagggtgtc gatcatgcca
      541 gtctgctcgc gtgcccgccc ctggcccgaa cgtggattga ggatcacttt gacgcgcatg
      601 ggtctgcttt acaccccttg ccaaacatgt tatactgaca ttcgcgttat gttacgtcaa
      661 gttgaccgcg gtggcaagac ggcaacacac ggtcacgggc gcgcggcggc tggcctgggc
      721 gcgccactgt tcaggagcgg gcattatgag tgcagatgat tccgtcgtca acaacggcga
      781 ggttgacaac acgaacgcgg gcgcgcctgt cgctgacggc aaccgcacct ttctggtcct
      841 ggcggcgctg ctgaccggaa cgctgttgct cttctcgttg tttaccgtag gttacttgct
      901 gcttggtcgc gccggcgggc gctcggccga gatcgccgcc atcgagacgc aaaacgccgt
      961 catcctggcc accaacgccg ccgtcaccga aacgatcgcc gccatgaacg tcgaagcgac
     1021 cagccgcgct ggcgcgagcg tggccgccgt tgaggacacg gctaccccga tggccgcggc
     1081 gacggccgag gaagacaacg ccgccccggc cgcgacctct gaaggcccgg ccgttgactc
     1141 gcctgagagc atgacggcga caatcgagac gctggcggcc aacccgaccc tggccgccgc
     1201 ggcgacgagc gccgccgcgg gcgaaccagg ccccacggcg accccgccgg ccactggcga
     1261 agagggcacg ccggccgcta acacaaccgc cagcccggat gaaaccgcgg agagcgcgac
     1321 aacgccggtt gacgccacag cggcgtcggg cgaaacgagt gcggccggac aggacactcc
     1381 ttcagcgacg gcagcaactg ctgagccggg tgaggctacg tccgtgggca cggacgcggc
     1441 tacgtccgtg cccacggacg cgacttcaac cgcgggcgac gccacggcgg ccacagcaga
     1501 cgctacggcc aacgccacag cggccacagc caacgccaac gccaccacca cgcctttgcc
     1561 gacctccgcg gccggtgccg gcacgacggc cacggccacc agcagtgtct tgcccgtgcc
     1621 cacggccacg gaagcaggcc gcgtgccggc cgtcggcgcc acggccacgc cggcgtccac
     1681 agtcgtcctg ctgggcgtga cgcaggaggc caccggcctc cccaccagct ccaccacaga
     1741 agcggaagca acgaccgtcg tcggccagcc gggcggttcg gcccccgtcg ccacgggtac
     1801 gcccacacca tccccctttg gcggcggcgg cgatgagtcc ggtggcggcg tgccggagac
     1861 accggcggcc acggctctgc cggccgatgc cggctacccg cccgcggcca cggagccggc
     1921 ggccgactac cccgcgccgg caacgtctac gccggtcagc aacgtgcccg accagccgac
     1981 ggccggcgcg gcgacgccaa cccaggtcgg cggagccacc cccaacctgc ccggctcgcc
     2041 cacgccgacg ctcgcgccaa cgaatacgcc cacactggta gcctctaaca cgccgacgct
     2101 cgtgccctcc agcacgccaa cgctggtcgc gtcgagcaca ccaacgctcg tgccgacgag
     2161 cacggcaacg accgtggcca cgactacagc cacgatggtg gccacgacaa cagcgacgac
     2221 aacagcgacg acgacggcga cggtcagcgc ctcgccgacg gccacgacca cggcgaccgc
     2281 cctgccggcg gtttccccca caacgacagc caccgcaact gccacggcgc tgcccggcac
     2341 ggccacaccc acgccaaccc cagatcccaa cgcgacggcc acggccactc aaacagccac
     2401 cgtcgcccca acagtcacca gcacggccgc cgcgacggcc acaggcacag tcatggcccc
     2461 gacgcccacg gccacgccca cgccgccggt gacgctgccc gaaactggcc tcgacgcctg
     2521 gggcatcgcc gctgttggtc tggctctggc cctcgtcgtc ttcggggcgc gccgtctgcg
     2581 tcgggcaggt taggtcgcgg agagggtatc gaaaaagagc cgggggctgg ttgcccccgg
     2641 ctctttctat ttccactgac ca
//
LOCUS       DAOSMQ010000454         2639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38851_length_2639_cov_3.825464, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000454 DAOSMQ010000000
VERSION     DAOSMQ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38851_length_2639_cov_3.825464"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PK829_12685"
     CDS             <1..1073
                     /locus_tag="PK829_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU42114.1"
                     /translation="LPDGGPQAVLGLSPLHRPLQTAAGLLGNNDLISPADKAELTLFF
                     TSGLIDYALTRRELDQHTVYDYARRRGVSEDAITNMLIPVTEGIFFLPPERYSAMVLM
                     GLLAPAIKRPHTIRVGSFAGGMTEVMSGPIAETIVRRGGTVQYDITVERLAVEDGRVS
                     GVYVDGQLIAANHVVLATSLGAAQPLIREALGDHPWFEKMLALPTMPAVTLQIELDEP
                     ALPEDHVVFSPNTVLASYAEQSRTTFRDVPGRLSIDLGQPEKFIGRPPEEIFAAAMAD
                     ARRVGLNIEDNVRRYRVVDHPQDFYLLSPGAEALRPPQATPIPGLTLAGDYTKQALFC
                     SMEGAVMSGQAAAKAVARAAKA"
     gene            complement(1088..>2639)
                     /locus_tag="PK829_12690"
     CDS             complement(1088..>2639)
                     /locus_tag="PK829_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU42115.1"
                     /translation="VTVAASLYELGLRPRDLIIIAHTQNLESIFAFWGALRLGAIPSM
                     FPTLTEKLDAGVYMSSLAELARLSGVAAVLTTDAFAPVVRPRLPCPVYGAADLPAPTG
                     IESGPASAGRTFHPPAPAEIAFLQHSSGTTGLQKGVALSHAAVLNQMAAYSTALALHE
                     SDVVVSWLPLYHDMGLIAGFLLPLLQGVPLVLMSPFDWVQHPALLFRAIHDYHGTLCW
                     LPNFAYNHCARGIRRRDMDGLSAASMRLFVNCSEPVRHDSQTLFLERFAANGVRPEML
                     GVSYAMAENTFAVTQTPPGRPARLDTIDAATRIAGAAPPQLPDDPASAVRVSCGPPIP
                     GTQVRVLDDEGHTLPDRRVGQLAIRSDCLLTGYYRRDDLQPFTPDGWFLTGDRGYLAE
                     GEVYVVGRSKDLIINAGKNIYPADIEAIVNEVPGVHAGRAVAFGVADEREGTELVAVV
                     AETGVDDAGARATVSRAIRAAVAHRAGVTVSYVHLVGPRWLLKTSSGKIARAANRDKW
                     RRETMDAG"
BASE COUNT          424 a          832 c          939 g          444 t
ORIGIN      
        1 gcctacccga tggcgggccg caggccgtgc tgggcctgtc gccgctgcac cggccattgc
       61 agacggccgc cggcctcctg ggcaacaacg acctgatttc cccggccgac aaggccgagt
      121 taaccctgtt cttcacctcc ggcctgatcg actatgccct gacacggcgg gagctagacc
      181 agcacaccgt ctacgactac gctcgccggc gcggcgtcag cgaggacgcc attaccaaca
      241 tgctcattcc cgtcacggag ggcatcttct ttctgccgcc ggagcgctac tcggccatgg
      301 tgttgatggg cctgctggcc ccggccatca agcggccgca taccatccgc gtcggttcgt
      361 ttgccggtgg catgaccgag gtcatgtctg ggccaattgc cgagaccatt gtccggcgcg
      421 gcggcacagt gcagtacgat attacggtcg agcgattggc ggttgaggat ggccgcgtca
      481 gtggcgtcta cgtcgatggc caattgatcg cggccaacca tgtcgtcctg gccacgtcgc
      541 tgggcgcggc gcaaccgctc attcgcgagg cattgggtga ccacccctgg ttcgagaaga
      601 tgctggctct gccgaccatg ccggcggtga cgctgcaaat cgagttggat gagccggcgc
      661 tgcccgagga tcacgtggtc ttcagcccta acacggtgct ggcttcctac gccgaacaga
      721 gccggacgac ctttcgcgac gtgcccggcc gcctgtcgat tgacttgggc cagccggaga
      781 agttcatcgg ccggccgccg gaggagatct ttgccgcggc gatggctgac gcgcggcggg
      841 tgggtcttaa tatcgaggac aacgtgcgcc gctatcgcgt cgttgatcac cctcaggact
      901 tctatctatt gtcgcccggc gctgaggcgc tgcgcccgcc gcaggccacg cccatccccg
      961 gcctgacgtt ggccggcgac tacaccaagc aggcgctctt ctgtagcatg gagggcgcgg
     1021 tgatgtcggg acaggcagcg gccaaggccg ttgctcgcgc ggccaaggcg tagcgccacc
     1081 accgtcctta cccggcgtcc atcgtctcgc gccgccactt gtcccgattg gcggcgcgag
     1141 cgatcttgcc gctggaggtt ttcagcagcc agcgcgggcc gaccaggtgg acgtagctga
     1201 cggtcacacc ggcacggtgg gcgacggcag cgcggatggc gcggctgact gtggcgcgcg
     1261 cgccggcatc gtccacgccc gtctcggcca caacggccac tagctccgtt ccctcgcgct
     1321 cgtcggccac gccaaaggcc accgcccgcc cggcatggac gccgggaact tcgttgacga
     1381 tggcttcgat gtcggccggg tagatattct tgccggcgtt gatgatgagg tctttggagc
     1441 ggccgacaac gtagacttcc ccctcggcca gatagccccg gtcgccagtc agaaaccagc
     1501 cgtcgggcgt gaatggttgc aggtcgtcgc ggcggtagta gccggttagc aggcagtcag
     1561 agcggatggc cagttgaccc acgcggcggt cgggcagggt gtggccctcg tcgtccagga
     1621 cgcggacttg tgtgccgggg atgggcgggc cgcaggagac gcgcacggcc gaggcggggt
     1681 cgtcgggcag ctgtggtggc gcggcgccgg cgatccgcgt ggccgcgtcg atggtgtcca
     1741 ggcgggcggg gcggccgggc ggcgtttgtg tcacggcaaa ggtgttctcg gccatggcgt
     1801 agctgacgcc cagcatctcc gggcggacgc cgttggcggc gaagcgctcc aggaagagag
     1861 tctggctgtc gtggcgcacc ggctcggaac agttgacgaa aaggcgcatg gacgcggccg
     1921 agaggccgtc catatcgcgg cggcggatgc cgcgggcgca gtggttgtag gcgaagttag
     1981 gcagccagca gagcgtgccg tggtagtcgt ggatggcgcg gaagaggagg gcggggtgct
     2041 gtacccagtc gaaaggcgac atgagcacga ggggcacacc ttgcagcagc ggcagcagga
     2101 agccggcgat gaggcccatg tcatggtaaa ggggcagcca gctcacaaca acgtcgcttt
     2161 cgtgtagtgc taaggctgtg ctataagcgg ccatttgatt gagcacagcc gcgtggctaa
     2221 gcgcgacgcc tttttgcagg ccggtcgtgc cgctgctgtg ttgcaggaag gcaatctcag
     2281 ccggcgcggg cgggtggaac gttcgtcccg cgcttgccgg gccgctctct atgccggtgg
     2341 gcgcgggcaa gtcggccgcg ccgtagaccg gacagggcag gcgcggccgt accacaggcg
     2401 cgaaagcgtc ggtagtcagc acggcggcca cgcccgacag ccgcgccagt tcggccaggc
     2461 tgctcatata gacgccggcg tccagcttct cggtcagtgt ggggaacatc gacgggatgg
     2521 cccccaaccg cagcgcgccc cagaaggcga aaatgctttc cagattctgt gtgtgggcga
     2581 tgatgatgag gtcacgtggg cgcaggccaa gttcgtatag cgacgcagcg accgtcacg
//
LOCUS       DAOSMQ010000455         2635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38919_length_2635_cov_3.632946, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000455 DAOSMQ010000000
VERSION     DAOSMQ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38919_length_2635_cov_3.632946"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PK829_12695"
     CDS             complement(<1..426)
                     /locus_tag="PK829_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42116.1"
                     /translation="MRNDPFSIHFWLAAALIIIVIGLAACGGAPAVDTAPTATFPPPP
                     AETTAAAPVAELPTGYPVATPADALPTGYPGESATSAPLPTPGPGNAATAVPSSDAVF
                     MPMIGQGVESPAEAIAPTPEPPTATATAEPPTATPEPPTA"
     gene            675..1388
                     /locus_tag="PK829_12700"
     CDS             675..1388
                     /locus_tag="PK829_12700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU42117.1"
                     /translation="MEVQFAVAKVTKWATSESGDTLEMIERPHGGISMVLVDGQRSGK
                     SAKLISNIVARKAVQLLSEGVRDGAAARAAHDYLYTNRGGKVSATLNMISVDTLTRTL
                     VISRNNEAPVLVHTAAGGFYLLDQPSVAVGVHRNTKPVISEVPIEVNTIVVAFTDGLR
                     HAGTLSGRGMAYDPDAAFRQMVGEGIGDAHTICDRLLAEALAMDNGRARDDISVMAIS
                     VLESADGDEVRRLSGRLIL"
     gene            1470..1871
                     /locus_tag="PK829_12705"
     CDS             1470..1871
                     /locus_tag="PK829_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42118.1"
                     /translation="MTLSPAPPPPRSPASPLRVAVVGPCASGKSTLVAALRAAGYEAR
                     HPAQEHSYVPDMWRRLVDPDVLIYLDISYEALLARRPHFGEPEYLEREKARLAHAREH
                     ADLVVDTSELAAEEVRQRVLAALAGGASTAG"
     gene            complement(1879..>2635)
                     /locus_tag="PK829_12710"
     CDS             complement(1879..>2635)
                     /locus_tag="PK829_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42119.1"
                     /translation="MPEHPGDISTTATAVTSSPAALATQVETPSVAEATATAATRATP
                     SRTTGDSPTSPLLPEPAATATATPPTPSPTVTPIPTSAEVDGWLVYENDFLGYRFSYP
                     PEARIRVQGFTGFPMDELPENLTVEEYQAQLEAEYPDNLCVTVAYQAVFIAFVPSYDT
                     VGKYTVPCGVTGVGDFEIKTEEVSFIIDGRQVSGSLHRMFGGEGNWRGEFTLLGDGDD
                     LGIHYGSANDATHEEFLDAQETMMQIVLSLRLD"
BASE COUNT          468 a          811 c          927 g          429 t
ORIGIN      
        1 ggccgtgggc ggctccggcg tggccgtggg cggttcggct gtggccgtgg cggtgggtgg
       61 ttccggcgtc ggcgcgatgg cttcggccgg cgactcgacg ccctggccga tcatgggcat
      121 gaagacggca tcgctggacg gcacggccgt ggccgcgttg ccggggccgg gcgtgggcaa
      181 tggcgcggac gtggccgact caccgggata gccggtgggc aacgcgtcgg ccggtgtggc
      241 gacagggtag ccggttggta gctcggccac gggcgcggcc gcggtggtct ccgccggcgg
      301 ggggggaaaa gtggctgtcg gggctgtgtc cacggccggc gcgccgccac aggcagcgag
      361 gccgatgacg atgatgatca gcgcggcagc aagccagaaa tgaatactaa aggggtcgtt
      421 tctcatagca ataataaggc gcggccatcg ccgcgcccga taactcatct tactccggtg
      481 ggcgcgggtc gcaaccggcg ctcatgcccg gctcatgcct ccgggccaag cagaaagcgc
      541 aaggcaagtg gacgcgcgcc cgccaggcga tctcattgtg gagcgcggcg gaacgtggcg
      601 cgaagggaag ttctcgtgac gaatcatgtg aagacctgct atcataaaga cgtcattatc
      661 cccgaaaaag ttctatggaa gttcaattcg cagtcgccaa ggtcaccaag tgggccacca
      721 gcgagagcgg cgatacgctg gagatgatcg agcggccgca cggcggcatc tccatggtgc
      781 tggtggacgg ccagcgcagc ggcaagagcg cgaagctcat cagcaacatc gtcgcccgca
      841 aggcggtgca gttgctcagc gagggcgtgc gcgacggcgc agccgcccgc gccgcccacg
      901 actacctcta taccaaccgc ggcggcaagg tctcggccac gctgaacatg atctccgtcg
      961 atacgctgac gcggacgctg gtcatcagcc gcaacaacga ggccccggtg ctggtgcata
     1021 cggctgccgg cggcttctac ctgctcgatc agccctcagt cgccgtcggc gtccaccgca
     1081 acaccaagcc ggtcatcagc gaagtgccga ttgaggtcaa caccatcgtc gtggccttca
     1141 ccgacggcct gcgccacgcg ggcacgctga gcggccgggg catggcctat gaccccgacg
     1201 ccgccttcag gcagatggtc ggcgagggca tcggcgacgc ccacacgatc tgcgaccggc
     1261 tgctggccga ggcgctggcg atggacaacg gccgcgcccg cgacgacatc agcgtgatgg
     1321 ctatctccgt gctggaaagc gccgatggag acgaagtgcg gcggctgagc gggcgactga
     1381 ttctgtaatt gggaattagg aatgaagagg aagaacctca cgccaaggac gccaagggag
     1441 caaagacgca aagaagaaac caatcattga tgactctctc ccctgctccc ccgccccccc
     1501 gctcccctgc atcccccctg cgcgtggccg tcgtcggccc gtgcgccagc ggcaagagca
     1561 cgctcgtggc ggcgctgcgg gcggccggct acgaagctcg ccacccggcg caggagcact
     1621 cctacgtgcc cgatatgtgg cggcggctag tcgatcccga cgtgctgatc tatctggaca
     1681 ttagctacga ggcgctgctg gcgcggcggc cgcacttcgg cgagccggag tacctggagc
     1741 gggagaaggc gcggctggcc cacgcgcgcg aacacgccga tctggtggtg gacacgagtg
     1801 agttggccgc cgaagaagtg cgccagcggg tgttggcggc gctggccggt ggcgcgagca
     1861 cagcggggta aatcctgctt aatccaggcg caatgacaaa acgatctgca tcatcgtctc
     1921 ttgcgcgtcc aggaactcct cgtgggtcgc gtcgtttgcg ctgccgtaat ggatcccgag
     1981 gtcgtcgccg tctcccaaca gggtgaactc gccgcgccaa ttgccctccc cgccaaacat
     2041 ccgatggaga gagccggaca cttgccggcc atcgatgata aagcttacct cttccgtctt
     2101 gatctcgaaa tcaccgacgc cggtgacacc gcagggcacg gtgtatttgc caacggtatc
     2161 gtaagatggc acgaaggcga taaagacagc ctgataggcg accgtcacgc agagattgtc
     2221 aggatactcg gcctccagtt gggcctgata ctcctcgacg gtcaggtttt ccggtagctc
     2281 gtccattggg aagccggtaa agccctggac acgtattctc gcctccggcg gatagctgaa
     2341 gcgatagccg aggaagtcgt tttcgtagac gagccagccg tccacctcgg ctgacgtggg
     2401 gatgggcgtc acggttggcg agggcgtcgg cggtgtggcg gtggccgttg ccgccggttc
     2461 gggcaggagc ggggaagtgg ggctgtcgcc ggttgtgcgc gagggcgtag cgcgtgtggc
     2521 ggccgtggcc gtggcttcgg cgacggaggg cgtctcgacc tgggttgcga gcgcggccgg
     2581 actgcttgtg accgctgtag ctgtcgtgga gatgtctcct ggatgctctg gcata
//
LOCUS       DAOSMQ010000456         2631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_38999_length_2631_cov_3.565217, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000456 DAOSMQ010000000
VERSION     DAOSMQ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38999_length_2631_cov_3.565217"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK829_12715"
     CDS             <1..263
                     /locus_tag="PK829_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42120.1"
                     /translation="VKGTAKYKAAGGEREFQVELENARALKGKTLDVYANGMRVGSFK
                     VSALGAGRLSRNTDLGQAVPQISAGSKVQIKWGSILVAQGSF"
     gene            complement(351..1169)
                     /locus_tag="PK829_12720"
     CDS             complement(351..1169)
                     /locus_tag="PK829_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU42121.1"
                     /translation="MEASSYSSNLKRQTLAEQLAETLREAILAGRWRAGEALPTEPEL
                     AAQFGVSRAVVRDGTRMLAAQGLVQAQHGRGVFVTASPVAAFGDALLLALRRAGASAW
                     DVERFEQMVMPEVQAEAARQATDADLVAIQRLAEAYHDTFAAVTRDSWGRDRLSAGER
                     ERIMLAFQALYGAIFAATHNALWGLLAEPLLRLRAPRSWQSGEMTVEEFIAHERRIID
                     ARVTAIVARDPDRAYDTARSSAAAPPDIASTLRAALQATPVAEIATIPLPLPSR"
     gene            complement(1138..2445)
                     /locus_tag="PK829_12725"
     CDS             complement(1138..2445)
                     /locus_tag="PK829_12725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU42122.1"
                     /translation="MNSRLTVRAVRARYYAITFLVWFATALPLALSVLLAQARGLSLF
                     EVSVALGLYSLTIVLLEVPTGGLADAVGRKRVALLAFALAALYNVAFLLAFSFPMLLL
                     AMILYGVSRALSSGALDAWFVDALQAADANVDLQPALAGAETVGLLALGLGTMAGGVL
                     PKLFAWLPADGTAILTPLAVPLVAALAVKLLTVVAIAVLVREERPVGGSWRAGFRAVP
                     ALVRDALALSRANPRLLLLMSASFVAGLAIISLEALWQPFFAALPEVGQSSAAAPLFG
                     VIMAGNFLVGMVGNLLSVPLARHWGGRYARVSAVARLLQGASILALAATGALVPAAGF
                     FWLAYLMGGVGLSPHATLLNAEIPAERRSAMLSVQSLAAYLGSFVGGAALGYVAEHVA
                     ISAAWLIAGALTVVSLAPYLILDARRDRRPEGDCGSEQLLQQP"
BASE COUNT          489 a          863 c          957 g          322 t
ORIGIN      
        1 acgtcaaggg cacggccaag tacaaggccg ctggcggcga gcgggagttc caggtcgagc
       61 tggaaaacgc cagggcgctg aagggcaaga cgctggacgt gtacgccaac ggcatgcgcg
      121 tgggtagctt caaggtgagc gcgttgggcg cgggccgcct cagccgtaac actgacctgg
      181 gccaggccgt gccgcagatt agcgccggca gcaaggtgca gattaagtgg ggcagcatcc
      241 ttgtggcaca gggcagcttc taagacagag tccaccccat gaacaaggag cgcgcctcct
      301 cggctcagcc gagggggcgc ttcttgtttg gacaggctca gctggttgtc tcagcgcgag
      361 ggcaggggca gggggatggt ggcgatctca gcgacggggg tggcttgcag ggcggcgcgc
      421 aaagtagacg cgatgtccgg cggcgcggcg gccgatgacc tggcggtgtc ataggcgcgg
      481 tcggggtcgc gggcgacgat ggcggtgacg cgggcatcga tgatccgccg ctcgtgggcg
      541 atgaactcct cgacagtcat ctcgcccgat tgccaggagc gcggcgcgcg cagacgcagc
      601 agcggctcgg ccagcaggcc ccagagggcg ttgtgggtgg cggcgaagat ggccccgtag
      661 agcgcctgaa aggccaacat gattcgctcg cgttcgccgg ccgagagccg gtcgcggccc
      721 caactgtcgc gcgtcacggc ggcgaaagtg tcgtgatatg cctcagccag tcgctgtatg
      781 gcgaccaggt cggcatcggt cgcctgccgc gccgcctcgg cctgcacttc cggcatgacc
      841 atctgctcaa agcgctccac gtcccaggcc gacgcgcccg ctcgccgcag ggccagcagc
      901 agcgcgtcgc cgaaggcggc cacgggcgac gcggtgacga agacgccccg gccgtgctgc
      961 gcctggacca acccctgggc cgccagcatg cgcgtgccgt cgcggacaac cgcccggctg
     1021 acgccgaact gcgccgccag ttctggctcg gtgggcaggg cctcgcccgc ccgccagcgg
     1081 ccggccaaaa tcgcctcgcg gagcgtctcg gccagttgct cggccaatgt ctggcgctta
     1141 aggttgctgg agtagctgct cgcttccaca gtcgccctcc ggacggcggt cgcgccgcgc
     1201 gtcgaggatg agatagggag ccaatgatac cacggtcagc gccccggcga tgagccacgc
     1261 cgccgaaatg gcgacgtgtt cggccacgta gcccagggcc gcgccgccaa cgaagctgcc
     1321 caggtaggcg gccagcgatt gcacggagag catggccgag cgccgttcgg ccggaatctc
     1381 ggcgttgagc agcgtcgcgt ggggcgacag gccgacgccg cccatcaggt aggccagcca
     1441 gaagaagccg gccgccggaa ccagcgcgcc ggtcgcggcc agggctagga tcgacgcgcc
     1501 ctgcaacagg cgggcgacgg cgctgacccg cgcgtagcgc ccgccccagt gccgcgccag
     1561 gggcaccgac agcagattgc cgaccatgcc gacgaggaag ttgccggcca tgatgacgcc
     1621 gaacaggggc gcggccgcgc tgctctgacc cacttccggc agggcggcga agaacggctg
     1681 ccacagcgct tcgaggctga tgatggccag cccggcgacg aacgaggccg acatgagcag
     1741 cagcaggcgc ggattggcgc ggctgagggc cagggcgtcg cgcacgaggg cgggcacagc
     1801 gcggaagccg gcccgccagg agccgccaac cggccgctcc tcgcgcacca gcacggcgat
     1861 ggccacgaca gttagcagct tgaccgccag cgcggccacg aggggcacgg ccagcggcgt
     1921 caggatagcc gtgccgtcgg ccggcagcca ggcgaagagc ttgggcagca caccgccggc
     1981 catcgtgccc agccccagcg ccagcaggcc gaccgtctcc gccccggcca gcgccggctg
     2041 gagatcgacg ttggcgtcgg ccgcctggag ggcatcgaca aaccaggcat cgagcgcccc
     2101 ggacgacagc gcccggctga cgccgtagag gatcattgcc aacaagagca tggggaagga
     2161 gaaggccagc aggaaggcga cgttgtagag cgctgccagg gcgaaagcca gcaaggcgac
     2221 gcgcttgcgg ccgacggcat cggccagccc accggtgggc acttccagca ggacgatggt
     2281 cagcgaatag agacccaggg cgacgctgac ctcgaacaga ctcagcccgc gggcctgggc
     2341 cagcagcacc gacagggcca gcggcagggc cgtggcgaac cagaccagga aggtaatggc
     2401 gtagtagcgg gcgcgaacgg cgcgaacggt cagacgtgaa ttcatcaggt catcatatga
     2461 catgatgtca tcataacacg caagacgatg gcctgtcaag gggttaggaa atgatgaact
     2521 gcgatgccgc gagcttggcg tttacagagg tgacagaaga ggaggtctag ctcgcacggc
     2581 tcaataagcg tcataaaccg cgtcctcgtc gttatcccag acccgcgcca g
//
LOCUS       DAOSMQ010000457         2630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39028_length_2630_cov_3.102913, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000457 DAOSMQ010000000
VERSION     DAOSMQ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39028_length_2630_cov_3.102913"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK829_12730"
     CDS             complement(<1..256)
                     /locus_tag="PK829_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOU42123.1"
                     /translation="MKDAQKPDHISLIALVGHLKEGRFVIPNFQRDFEWEPWDISALM
                     RSIFLDYYIGSLLLWRGKSENFTALACEPIYAYKGNKKSWE"
     gene            complement(336..1724)
                     /locus_tag="PK829_12735"
     CDS             complement(336..1724)
                     /locus_tag="PK829_12735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOU42124.1"
                     /translation="MAKVEIMSPVDVAWLSMEEPTNLMMVNAIITFDRPLDFDRVRQV
                     LRYRWLRYHRFRQRVVRPPVPLLRPYWEDDPHFDLDLHLQRIALPAPGDRATLQEMVS
                     DLMSGPLDFARPPWKFILIENYGEGCAIMARIHHCLADGMALVAVLLAMTDFYPDAPL
                     TPGEPADDGPMAESWGGGLARDLGDSVGAARAAVGGLLRRARRRDAAVGLGEQGAGMG
                     LAAGRLLARSPDPHTRLKGKLGVLKRAAWADPIPLTDVKLIKNAFGGTVNDVLVAAVT
                     GGLRHYLVDRGDDVGDDSGGLEIRAAIPVNLRRDDDNGKLGNRFGLVFLTLPIHIEQP
                     LMRLAEVRRRMLELKRSAEAPVTLGILGAMGLGTDAFREFVVRMLEPKATLVLTNVPG
                     PPVPLYLAGQRIRDLMFWVPQAGRLGVGISILSYAGNVYVGVATDAGLVPEPDEIMLG
                     FYGEYDELLGLV"
     gene            1914..>2630
                     /locus_tag="PK829_12740"
     CDS             1914..>2630
                     /locus_tag="PK829_12740"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42125.1"
                     /translation="MSERPSSAVTCGRCGRQIGLARNFCPFCGAPLAEPPGPVANFYD
                     QLSELQRGLAAAFSLGEMKDLAFALGVVDFASLPGETKEDKARELALRMVRENRLAQL
                     VALCRRERPNRAWPAFGPTPEPAENPLAAQFSAGLRAQLEGRLADARQIFEGIQRIDP
                     NYPGLAAQLMAIRQEMGRGYVDIDGRIDPYSVLHPPAGLPGLPDPARPLPTGQARRGA
                     SPLLLVGAIVVLLLLAVAVWY"
BASE COUNT          466 a          883 c          839 g          442 t
ORIGIN      
        1 gttcccacga tttcttgttg cctttgtaag catagatagg ctcgcaggct aacgcggtga
       61 aattctccga ttttcctctc cacaacaata ggctgccaat gtaataatcc agaaagatgg
      121 aacgcattaa ggcactgata tcccacggct cccattcaaa gtcgcgttga aaattaggta
      181 ttacaaatcg gccctctttt aaatggccaa caagcgcaat aaggcttata tggtcaggct
      241 tttgtgcgtc tttcataacg gccaacctct ctcttggcag ttccttgaaa caaatcgacg
      301 gtgtcgacgg gtcagcaacc catattcacg aaacgctaga ccaaccccaa caactcatca
      361 tactccccat aaaaccccag cataatctca tccggctccg gcacgaggcc ggcgtcggtg
      421 gccacgccga cgtagacgtt gccggcgtag ctgaggatgc tgatgcccac gcctaaccgg
      481 cccgcctgtg gcacccagaa catcaggtcg cggatgcgct gcccggccag atagaggggc
      541 acaggcgggc caggcacgtt ggtcagcacc agcgtggcct ttggctccag catgcgcacg
      601 acgaactcgc ggaaggcgtc ggtgcccagg cccatcgccc ccagaatgcc cagcgtcacc
      661 ggcgcttcgg ccgagcgctt tagctccagc atccgccgcc gcacctcggc cagacgcatc
      721 agcggttgct cgatgtggat cggcagcgtt agaaagacca gcccaaagcg gttgcccagc
      781 ttgccgttgt cgtcgtcgcg gcgcagattg accgggatgg cggcgcgaat ctccagcccg
      841 ccgctgtcat cgcccacgtc gtcccccctg tccaccaggt aatggcgcag cccgcccgtc
      901 acggcggcga cgagcacgtc gttgaccgtg ccaccgaagg cgttcttaat gagcttcacg
      961 tcggtcaggg gaatggggtc ggcccaggcg gcgcgcttca ggacgccgag cttgcctttc
     1021 aggcgcgtgt gcgggtcggg gctgcgggcc agtagacggc cggcggccag ccccatgccc
     1081 gcgccctgct cgcccagccc cacggcggcg tcgcggcgac gcgcgcggcg cagcagcccg
     1141 ccaacggcgg cgcgggccgc gccgacgctg tcgcccagat cgcgagccag cccgccgccc
     1201 caactttcgg ccatggggcc atcgtcggcc ggctcgcccg gcgtcagcgg cgcgtcggga
     1261 tagaaatccg tcatggccag caacacagcg accaaagcca tcccatcggc cagacaatgg
     1321 tggatgcggg ccatgatggc acagccctcc ccgtagttct cgatgaggat gaacttccac
     1381 ggcggacggg cgaagtccag cgggccactc atcaggtcgc tgaccatctc ttgcagtgtg
     1441 gcgcggtcgc cgggagcggg cagagcgatg cgctgcaaat ggagatcaag gtcaaagtgg
     1501 gggtcgtcct cccaataggg gcgcagcaag ggcacggggg gccggacgac gcgctggcgg
     1561 aagcgatgat agcgcagcca gcgatagcgc agcacctgcc ggacgcggtc gaagtcgagc
     1621 ggccggtcaa aagtgatgat ggcgttgacc atcatcagat tggtcggctc ttccatgctg
     1681 agccaggcga catccacggg ggacatgatc tcgactttgg ccacgttctt gctgtacctc
     1741 cgcgcgcgtg atgacgttat tatagcgccc tgcccgcgac tgcgcgacgc ggcccctgcg
     1801 cctggcgatt gccggagggg ggcaagcgaa aaagcgccgt tgcgcgttcc gcctgctgcg
     1861 gcgacaatag cgcgtctgat cctttggcgc ggcaacaggg gcagcgaagc gtgatgagcg
     1921 agcgaccttc ctcagcagtc acctgcgggc ggtgcggccg ccagattggc ctggcccgta
     1981 acttctgccc cttttgtggc gcgccgctgg ccgagccgcc cggcccggtg gccaactttt
     2041 acgaccagtt gtcagagcta cagcggggcc tggccgccgc cttcagcctg ggcgagatga
     2101 aagacctggc cttcgctctg ggcgtcgtcg atttcgcctc gctgccaggc gagaccaaag
     2161 aggacaaggc gcgggagctg gcgctgcgca tggtgcgcga gaaccgcctg gcgcaactgg
     2221 tcgccctgtg ccggcgcgag cggcccaatc gcgcctggcc cgcctttggg ccgaccccgg
     2281 agccggccga gaatcccctg gcggcgcagt ttagcgccgg cctgcgcgct caactcgagg
     2341 gccggttggc cgacgcgcgg cagatcttcg agggcatcca gcgcatcgac cccaactatc
     2401 cggggctggc ggcccaattg atggccatac gccaggagat gggcaggggc tatgtggaca
     2461 tagacggccg gattgacccc tactccgtcc tgcacccgcc tgccggtctc cctggcctac
     2521 cagaccccgc ccgcccactg ccaaccggcc aagcgcgccg cggcgcgtcg ccgctgctgc
     2581 tcgttggggc cattgtcgtg ctgcttctgc ttgccgtggc ggtgtggtac
//
LOCUS       DAOSMQ010000458         2628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39048_length_2628_cov_3.818888, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000458 DAOSMQ010000000
VERSION     DAOSMQ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39048_length_2628_cov_3.818888"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PK829_12745"
     CDS             <1..731
                     /locus_tag="PK829_12745"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU42126.1"
                     /translation="DAIQNITGLDKMLASYPRELLAFFGDMADLASARGYFGIYYSSY
                     MPIIVGIFAVGAAAALLAGDEERGTLDLTLAQPVGRSALFWGRWLGFVAATALILLLG
                     YLGWAVTLPFSALDVTAGALLAAFVPILGMLLFFGGLSLLLSMVLPSARIASMSAGGL
                     LVANWLLVGLAKLNPDLQPIVDLTPFAYFQGGEALVDMNWGWAVGLIVAGFVLAAAAW
                     ALFLRRDIRVGGERSWSWRGLRRA"
     gene            863..1312
                     /locus_tag="PK829_12750"
     CDS             863..1312
                     /locus_tag="PK829_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HOU42127.1"
                     /translation="MSERLQFTVDKFTFTIPTDRLYHPEGLWVMERAGQLVAGVTDFF
                     QQTNGDVVLAEIATAGTAIAAGERLGDIETIKVDIELPSPVSGTVVKVNDQLELAAEI
                     INQAPYGDGWLVVIAPAAWNEERTALMSPEAYAAHSHARALKEIGKQ"
     gene            1309..1728
                     /locus_tag="PK829_12755"
     CDS             1309..1728
                     /locus_tag="PK829_12755"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOU42128.1"
                     /translation="MSDARKVVIVPCSGIGKTYGTVSREAAYEVIEDLRPDETQLVAL
                     SMLVLGDEGARAAVDGSQTIAIDGCKLACASKMVRDSGGTVARSYAVLDAYRRHKAFK
                     PAGIAELNEGGLQLAHALAEEIAGEIDILTRGEADNA"
     gene            1721..2464
                     /locus_tag="PK829_12760"
     CDS             1721..2464
                     /locus_tag="PK829_12760"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOU42129.1"
                     /translation="MPELPQKKVGIIACSGEEMAEGTVTRLAALQVLESLRPDDTVTI
                     CLPLFLAGGEGDRAFARYYPTIAVDGCDRRCAARATEQFSGKPAASIVVTDVVREMGL
                     EPPAGCRRLNDAGQAAVAETAGRVAGLVDELLDRRWSRRKGEFIDAEPIPVPVVPAAK
                     TTAGCACGSGIPVQTVAIDGTPVTLVALPPLMEQLRDAGKTPSPETIHELLEQVALYN
                     PIPAGEQDAYLAALTRTYAAFWGQLEAVV"
BASE COUNT          431 a          825 c          923 g          449 t
ORIGIN      
        1 acgacgccat ccagaacatc accgggctgg acaaaatgct ggccagttac ccgcgcgagc
       61 tgctggcctt cttcggcgac atggccgatc tggcctcggc caggggttat ttcggcatct
      121 actacagcag ctacatgccc atcatcgtcg gcatcttcgc cgtcggcgcg gcggcggcgc
      181 tgctggccgg cgacgaggag cgcggcacgc tcgacctgac cctggcccag ccagtcggcc
      241 gctcggcgct gttctggggg cgctggctgg gcttcgtcgc cgcgacggca ctcatcctgc
      301 tgctgggcta tctcggttgg gcggtgacgc tgccgttctc ggcgctggac gtgaccgccg
      361 gggcgctgct ggcggcgttc gtgcccatcc tgggcatgtt gctgttcttt ggcgggctgt
      421 cgctgctgct cagcatggtg ctgccgtcgg cgcgcatcgc gtcgatgagc gccggcgggc
      481 tgctggtggc caactggctg ctggttggcc tggccaagct caaccccgac ttgcagccca
      541 tcgtcgatct gacgccgttc gcctacttcc agggcggcga ggcgctggtg gacatgaact
      601 ggggctgggc cgttgggctg atcgtcgccg gctttgtgct ggcggccgcg gcctgggcgc
      661 tgttcctgcg gcgcgacatc cgcgtgggcg gggaacgtag ctggagctgg cgcggtctgc
      721 gacgggcata ggcgcaggcc gcccatttgt cacctcaaat ccatgacaat tgtcactact
      781 gaccgtcggg cgcggggctt ataattcaac gcagattgaa ttatcgccac ggcttcgcgc
      841 cgcgacggta ggagcgaggc agatgtcaga gcggttgcaa tttacggtcg acaagttcac
      901 cttcaccatt ccgaccgacc gtctatacca cccagaaggg ttatgggtga tggaacgcgc
      961 gggccaactg gtggccggcg tgaccgactt cttccagcag accaacggtg atgtggtctt
     1021 ggccgagatc gccacggcgg gcacagccat cgccgcgggc gaacgtctgg gcgacatcga
     1081 aacgatcaag gtcgatatcg aactgccatc acccgtgtcg ggcacggtcg tcaaggtcaa
     1141 tgaccagttg gaactggcag ccgagatcat caatcaggct ccctacggcg atggctggct
     1201 ggtggtgatt gcgccggcgg cgtggaatga agaacggacg gccctgatgt cgccggaagc
     1261 gtacgccgcg cacagccacg cccgcgcgtt gaaggagata ggcaagcaat gagcgacgcc
     1321 aggaaagtcg tcatcgtgcc gtgcagtggc atcggcaaga cctatggcac cgtcagtcgc
     1381 gaggcggcct atgaggtgat cgaagacctg cggccggacg agacgcaact ggtcgccctg
     1441 tccatgttgg tattgggcga cgaaggggcg cgggccgcgg tagatggcag ccagacgatc
     1501 gccatcgatg gctgcaagtt ggcctgcgcc agcaagatgg tgcgcgatag cggcggtacg
     1561 gtcgccaggt cgtatgccgt gctggatgct taccgccgcc acaaggcgtt caaaccggcc
     1621 ggcatcgcgg aactcaacga gggtggcctg caactggcgc atgccctggc ggaggaaatc
     1681 gccggcgaga tcgacatcct gacgcgtggg gaggcggaca atgcctgagt tgccacagaa
     1741 gaaggtcggc attattgcct gttcgggcga ggagatggcc gaagggacgg tcacgagact
     1801 ggccgcattg caggtgctag aaagcttgcg gcccgacgac acggtgacca tctgcctgcc
     1861 gctctttctg gccgggggcg agggcgaccg cgcctttgcc cgctactacc cgacgatcgc
     1921 cgtggacggc tgtgaccggc gctgcgcggc gcgggccacg gagcagttct ccggcaaacc
     1981 ggcggcgagc atcgtcgtca ccgacgtggt gcgcgagatg gggctggaac cgcccgccgg
     2041 ctgccgccgc ctgaacgacg ccgggcaggc ggccgtggcc gagacggccg gccgcgtggc
     2101 cggactggtg gatgagctgc tggacaggcg ttggagccgc cgcaaggggg agttcatcga
     2161 cgcggaaccc attcccgtgc cggtcgttcc ggcggccaag accacggccg gttgcgcctg
     2221 tggctcgggc atcccggtgc aaacggtggc catcgatggg acgccggtga cgctcgtggc
     2281 cctgccgccg ttgatggaac agctgcgcga cgcgggcaag acgccttcgc cggagaccat
     2341 tcatgaactg ctggagcagg tggcgctcta taatcccatt ccggccgggg agcaagacgc
     2401 ttatctggcg gcgctcacgc gcacctacgc cgccttctgg ggccaactgg aggctgtggt
     2461 atgagcgcga cggccgtcta tcacaccacc gtcacctggc gcggcgagca ttggggccac
     2521 atcgtcatgg gcaacggccc ggagatggcc ttctccgccc cgcccgacgc gcagggccac
     2581 cccggcgtat tgacacccga ggacgccttt gttgccgcca tcaactcg
//
LOCUS       DAOSMQ010000459         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39132_length_2623_cov_4.001947, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000459 DAOSMQ010000000
VERSION     DAOSMQ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39132_length_2623_cov_4.001947"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2137)
                     /locus_tag="PK829_12765"
     CDS             complement(<1..2137)
                     /locus_tag="PK829_12765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU42130.1"
                     /translation="MTPSFHVDARAERRLLALILTLFVLLGFSYALITPVFEASDELW
                     HYPMVRHLADGNPLPVQVFDPALAGPWNQEASQPPLYYTLAAALTFWIDTGDMAQVRW
                     LNPHVDNGVITADGNTNLVVHDPAANPWAGTRLAVRVIRLFSVLLGAATVYLTYRMAT
                     EIVPGRPGVALLAAALNAFLPMFLFISGAVNNDNLAVPLASLALLLLIRIVIRRRGGY
                     FPVRWTEWAAVGAVLGLAALTKEGTLGLFPLAFGTAVVAAWQRMEAEQGGGGAGERGS
                     KKALLTAVRNVAVALVLVAAPAAVIAGWWYARNIALYGDWLGWNAFIAVLGQRAAPAS
                     LAQLWGERRGFMMSFWGLFGGVNVPMPLWIYAVLNVFLVLCVAGFVLYLFRIGRSEWR
                     EWRASPSPNPSRREGDETPSPLGRGLGRGADAAGQRLLWAVERHFGLIAIGLFAAAVV
                     YGLVQWATTTWSSQGRLVFTAISVLCTLMACGWMSLLDLTGLRKPVRSGLFAAPAVFL
                     FFVAAAAPWLWIRPAYVPPAYPGPLATAADIVFGDAMRLASYEVQSDADPLRPGNTVR
                     VRLEWEARQPMTRDWSVFVHLNDPVLGRPIAQRDMFPGQGLLATRLLSPGQRLVNDYV
                     LTLPATAVAPAELELAVGLYDFATGERLPVTVANGAANAGPDTVTLATVPLQPRDGAL
                     PNPTSLFVEHGLELVGFAVEPRRVAAGDAL"
     gene            complement(2302..>2623)
                     /locus_tag="PK829_12770"
     CDS             complement(2302..>2623)
                     /locus_tag="PK829_12770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019060425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42131.1"
                     /translation="VSLRRGTLMAALAHGRPLITTAPGEADAAPELRHGDNVWFVPPG
                     DPAALAAAVSTLSADPAARARLGHGAREVAGLFDWGAIAGQTADFYGVLVSSDGGRTL
                     TQGR"
BASE COUNT          478 a          917 c          887 g          341 t
ORIGIN      
        1 ccagcgcgtc gccggcggcc acgcgccgcg gttcgacggc gaagccgacg agctccaggc
       61 cgtgctccac aaagagcgag gtcgggttgg gcagcgcgcc gtcgcgcggt tgcagtggca
      121 ccgtggccag cgtgaccgtg tccgggccag cgttggccgc gccattggcg accgtcaccg
      181 gcagccgctc gcccgtggca aagtcgtaga ggcccacagc cagctccagt tcggccgggg
      241 cgacggccgt agccggcagc gtcagcacat aatcattgac cagccgctgt cccggtgaca
      301 gcaggcgcgt ggccagcagg ccctgaccgg ggaacatgtc gcgctgggcg atgggccggc
      361 cgagcaccgg gtcgttgagg tggacaaaga cgctccaatc gcgggtcatc ggttgccgtg
      421 cttcccattc cagccggacg cggacggtgt tgcccggccg gagcggatct gcgtccgatt
      481 gtacctcata gctcgccagg cgcatggcgt cgccgaagac aatgtcggcg gctgtggcca
      541 gcgggccggg gtacgccggc ggaacgtaag ccgggcgtat ccatagccag ggagccgccg
      601 cggcgacgaa gaagaggaac acggccgggg cggcgaaaag gccagacctg acaggttttc
      661 tcaggcctgt caggtctaga aggctcatcc acccacaggc catcagcgtg cagagcaccg
      721 aaatggccgt aaagaccagc cgcccctgcg acgaccacgt ggtcgtcgcc cattgcacca
      781 gcccatagac gacggcagcg gcgaagaggc caatggcgat aaggccgaag tggcgctcga
      841 cagcccaaag cagccgttgc ccggctgcat cagcccctct ccccaaccct ctccccaagg
      901 gcgagggagt ctcatccccc tccctccgag aggggttagg ggagggcgag gcccgccatt
      961 cgcgccactc gctccgccct atccggaaca gatagagcac gaagccggcg acgcacagga
     1021 cgaggaagac gttgaggacg gcgtagatcc acaacggcat gggcacgttg acgccgccga
     1081 acaggcccca gaacgacatc atgaagccgc gccgctcgcc ccacagctgc gccagcgacg
     1141 ccggcgcggc ccgctgcccc agcacggcga tgaaagcgtt ccagcccagc caatcgccat
     1201 acagagcgat attgcgggca taccaccacc ccgcgatcac cgccgccggc gcggccacca
     1261 gcaccagcgc caccgccacg tttctaactg ctgtcagtaa cgctttctta ctcccccgct
     1321 cccctgcccc cccgcccccc tgctctgctt ccatccgctg ccacgccgcc accaccgccg
     1381 tgccgaatgc cagcgggaac agccccagcg tgccctcttt ggtcagcgcc gccagcccca
     1441 gcaccgcgcc cacggcggcc cactccgtcc agcgaacggg aaagtagccg ccgcgccgcc
     1501 ggatgacgat gcggatcagc aacagcagcg ccagcgacgc caggggcacg gccaggttgt
     1561 cgttgttgac cgcgccactg atgaacagga acatgggcag gaaggcgttg agcgccgccg
     1621 ccagcagagc cacgcccggc cgccccggca cgatctcggt ggccatgcgg taggtcagat
     1681 agaccgtcgc tgcgcccagc agcacggaga agaggcgaat gacacgcacg gccagccgcg
     1741 tgcccgccca cgggttggcc gccgggtcgt ggacgaccag gttggtgttg ccgtcggccg
     1801 tgatgacgcc attatcgacg tggggattca gccagcgcac ctgcgccatg tcgcctgtgt
     1861 cgatccagaa ggtcagcgcc gcggccaggg tgtagtagag cggcggctgg ctggcctcct
     1921 ggttccaggg gccggccagc gcgggatcga agacctgcac cggcaggggg ttgccgtcgg
     1981 ccagatggcg caccatcggg taatgccaca actcgtccga cgcctcaaag acgggcgtga
     2041 tgagggcgta gctgaagcca agaaggacga agagcgtcag gatgagcgcc agcaggcggc
     2101 gttcggcgcg ggcatcgacg tggaatgacg gggtcattac cgggattgta gcagggctgg
     2161 gggcgttggg cacagccgcc gcggggcgat tggcctacga caaccctctc gtgggtgata
     2221 ggttccaggg gccagggaaa gaagctcacg caaaggacgc gaaggaagaa aagccagctt
     2281 cttcttctct tccttcgccc cttagcgccc ttgcgtgagc gtcctgcccc cgtccgaaga
     2341 aaccagaacc ccatagaaat cggctgtctg cccggcaata gcgccccagt cgaacagccc
     2401 cgctacttcg cgcgcgccgt ggcccagacg ggcgcgcgcc gccgggtcgg cgctcaacgt
     2461 ggacacggcc gcggccagag ccgccgggtc gccgggcggc acgaaccaga cgttgtcgcc
     2521 gtggcgcagt tccggcgcgg cgtcggcctc gcccggcgcg gtggtgatca gtggccggcc
     2581 gtgggccaga gcggccatga gtgtcccccg gcgcagggaa acg
//
LOCUS       DAOSMQ010000460         2622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39155_length_2622_cov_3.858590, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000460 DAOSMQ010000000
VERSION     DAOSMQ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39155_length_2622_cov_3.858590"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PK829_12775"
     CDS             <1..280
                     /locus_tag="PK829_12775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU42132.1"
                     /translation="DGAMVGCVFCGHDGRRGYLHHVVVAPAARGQGVGAAMVARALDG
                     LAALGIYKTHLDVFTSNELAIAFWQHTGWQRRDDIVRFSFNRSDDADI"
     gene            440..832
                     /locus_tag="PK829_12780"
     CDS             440..832
                     /locus_tag="PK829_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU42133.1"
                     /translation="MTKKVYVGNLSFSATEDQVRDLFSEYGEIQSIAMINDRDTGRFR
                     GFAFVEMEDSAANAAIKALNGKEVDGRELSVNEARPREERSGGGSFGGGSRGGGSYGG
                     GNRGGGGYGGGGNRGGGDRDRNRSRNSW"
     gene            968..1576
                     /locus_tag="PK829_12785"
     CDS             968..1576
                     /locus_tag="PK829_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOU42134.1"
                     /translation="MNNTEGLALPAEVVREFVIAGHFDLPRVQAMLAEEPRLLNAANE
                     WGPGDMETAIQGAAHTGQAAIAEYLLARGAPLELCTAAMLGRRADVATFLAADADAIH
                     SRGAHGIALLSHAAFSGDVGLVADLHSRGAREGASLALANAVAAGHKGVVAWLLEYAD
                     PDLTWLNWQGKTALDVALEAGQTAIADLLRSYGAESGTPAGD"
     gene            1589..2104
                     /locus_tag="PK829_12790"
     CDS             1589..2104
                     /locus_tag="PK829_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU42135.1"
                     /translation="MELDEMMAQMSLQAGRIRALADGVAAAQARWRPDAESWSILEVV
                     NHLADEEREDFRTRLDFILHRPGEAPPPIDPQGWVTARGYNQRDLAESIDRFLGERQT
                     SLAWLRGLGAPDWATIVSAPDGSWRIRAGDMAAAWVAHDLLHTRQLVELHWAWTQQAV
                     APYATDYAGSW"
     gene            2181..>2622
                     /locus_tag="PK829_12795"
     CDS             2181..>2622
                     /locus_tag="PK829_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42136.1"
                     /translation="MEARRTTSPLWRRWVAANAVAEAVGLGLTLGLTGLVIGRLESIG
                     GALGVLVAFVAAVLSGVIEATVVGLAQWWAMRPWVPGITRRAWWLATLAGALLAYVLG
                     YLPSTIMSLMATGDPAAAAPMTEPPQAVVLLLAAGLGAVAGAVLS"
BASE COUNT          388 a          783 c          975 g          476 t
ORIGIN      
        1 ggatggggcg atggttggct gcgtcttctg cggccacgac gggcggcgcg gctacctgca
       61 ccacgttgtc gtcgctccgg cggcgcgcgg gcagggcgtt ggcgcggcga tggtggcgcg
      121 ggcgctggac ggtttggcag cgctgggcat ctataagacc cacctggacg tgtttaccag
      181 caacgagctg gccatcgctt tctggcagca caccggctgg cagcggcgcg acgacatcgt
      241 gcgcttctcc ttcaaccgct ccgacgatgc cgatatctag cctggctggg ccaaaaatga
      301 gcacttgcca accacttttt gtagagtgtt ggcttttcgg cgaagagtgt tacaatatgt
      361 ttgcttttgt cgtccgggcc ggagcaggtg agtaccagct taccggcctg ttattagttt
      421 gtgtagagag agtttcaagt tgaccaagaa agtgtatgtt gggaatttgt cgttcagcgc
      481 caccgaagat caggtccgtg atctgttttc ggagtatggt gagattcagt cgattgccat
      541 gatcaatgac cgggacaccg gccgtttccg tggcttcgcg tttgttgaga tggaagattc
      601 ggccgcgaat gcggccatca aggctctgaa cggcaaggaa gtggacgggc gtgagttgag
      661 cgtcaacgaa gcccggccgc gtgaggagcg ttctggtggc ggcagcttcg gcggcggcag
      721 tcgcggtggc ggcagctacg gcggtggtaa ccgcggtggc ggcggctacg gcggcggcgg
      781 caaccgcggc ggtggcgatc gcgatcgcaa ccgcagccgt aacagctggt aagcgtcgcc
      841 ccccgttggg cgctattacc ttagtaggct aaaaagccgt cccgaacttc gcaaggagtc
      901 gggacggttt tttgatccct ggctataatc tcgccgtcgc cggctgtgcc ggcggcaagg
      961 agcgtgtatg aacaatacag agggtctggc tttgccggcc gaagtcgtgc gcgagttcgt
     1021 catcgccggg cacttcgatc taccgcgcgt gcaggcgatg ctggctgagg agccgcggct
     1081 gctgaacgcc gccaacgagt gggggccggg tgacatggag acggctattc agggcgctgc
     1141 ccacaccggg caggcggcca ttgccgagta cctgcttgcc cggggcgcgc cgctggagct
     1201 atgtacggcg gccatgctgg gccggcgcgc cgatgtggcg acgtttttgg ccgccgacgc
     1261 cgacgccatc cacagccgcg gcgcgcatgg catcgccctg ctcagccatg ccgccttcag
     1321 tggtgacgtg ggcctggtgg ccgaccttca cagccgcgga gcccgcgagg gcgcgtcgtt
     1381 ggccctggcc aatgccgtgg ccgccggcca taagggcgtc gtcgcctggc tgttggagta
     1441 tgccgacccc gatctgacct ggctcaactg gcagggcaag acagcgctgg atgttgccct
     1501 ggaggccggt cagacggcta tcgctgacct gctgcggagt tatggcgcgg agagcggcac
     1561 gccggcgggc gattgatagg aggatcgcat ggaactggac gaaatgatgg cgcagatgtc
     1621 gttgcaggcc gggcgtattc gggcgctggc cgatggcgtc gcggccgcgc aagcccgctg
     1681 gcggccggac gccgaatcgt ggtcgatcct ggaggtcgtc aaccatctgg ccgacgagga
     1741 gcgcgaggac tttcgcacgc ggctggactt cattctccac cggccgggcg aggccccacc
     1801 ccccatcgac ccgcagggct gggtgacggc gcgcggctac aaccagcgcg atctggccga
     1861 gtccatcgac cgctttctgg gcgagcggca gacgtcgctg gcgtggttgc gcgggctggg
     1921 cgcgcccgat tgggccacca tcgttagcgc gccggatggt tcgtggcgca tccgcgcggg
     1981 cgacatggcc gcggcctggg tggcccacga tctgctgcac acgcggcagc tggtggaact
     2041 gcattgggcc tggacgcagc aggcggttgc cccttacgca accgactatg ccggctcgtg
     2101 gtagccggcc gccggccagg cggcaacggg agcgccgcgg ccgcgtcgtg agaggggaga
     2161 acttcatcat gacaacaagt atggaagcca ggcgaaccac ttcgccgctg tggcggcgct
     2221 gggtggcggc caatgcggtc gccgaggcgg ttggactggg tctgacgctg gggctgaccg
     2281 ggctggtcat cggccgtctg gagagcatcg gtggggcgct gggggtgttg gtggccttcg
     2341 tcgccgccgt gctcagcggc gtcatcgagg cgactgtcgt cggtctggcg cagtggtggg
     2401 ccatgcgtcc gtgggtgccg ggcatcaccc gccgcgcctg gtggctggcg acgctggccg
     2461 gggcgctact ggcctacgtc ctcggctatc tgccttcgac gatcatgagc ctgatggcca
     2521 ccggcgaccc ggccgccgcc gcgccgatga ccgagccgcc acaggcggtg gtgctgctgc
     2581 tggccgcggg gctgggggcg gtggccgggg cggtgctgtc gt
//
LOCUS       DAOSMQ010000461         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39177_length_2621_cov_3.769680, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000461 DAOSMQ010000000
VERSION     DAOSMQ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39177_length_2621_cov_3.769680"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK829_12800"
     CDS             complement(<1..380)
                     /locus_tag="PK829_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU42137.1"
                     /translation="MRLLLLEREAALDALERAWRAVRDGPGQVALVGGEAGVGKSALA
                     GRLVGDRSTGDGAAVLWGACDALFTPRPLGPLYDMAAGAPALAAALANPTDRAAVFAA
                     VLDTLRARPTIAVFEDVHWADEATL"
     gene            628..1431
                     /locus_tag="PK829_12805"
     CDS             628..1431
                     /locus_tag="PK829_12805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU42138.1"
                     /translation="MSPRSAHAPLNGIDCAYEMAGDGPPVVLLHAGIADSRMWAAQFD
                     ALATNHTVIAYGRRGFGRTRPAPGPFNHVDDLAALLGYLGIKRTALVGCSQGSRIALD
                     FALAHPDQVSRIVLSAPSVSGFRYDGPPPRQADAIDAAEAAGDLARVNELEMQIWVDG
                     PRRAPDAVASSVRRLVAEMNAIALANEGVGEERPPDRPAAGRLGEVGAPVLLVVGELD
                     AAITLAVAEALAGGLPNVRRVDLLAAHLPNMEQPEAFNRAVGPFLGERA"
     gene            1764..>2621
                     /locus_tag="PK829_12810"
     CDS             1764..>2621
                     /locus_tag="PK829_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42139.1"
                     /translation="MTHSPHSPVIRAATALVLFAILVVGIGAWSQPVAAAPTCTTSCY
                     VSPTGSDANDGATAATAFKTIQKAIDTVQANGQVRVLPGNYSETATGRDVLGSGSYQF
                     GLFFEETAKNGVTLQGVTAADVNITDPSATQAVITTNATNNFGYSGVFVEADDVTIAG
                     VEIGDNTPSNNKTIEVIGDGFTFKNSFMNVSDGGSLYFGDWRYNDTTDVSYIQSYTVD
                     NNIFADGSSIDIASGAGYTGPVAGRTITNNEFSFSLAMYTAMGNVSWPSISFNGDTTT
                     VSWYTYPVGG"
BASE COUNT          452 a          923 c          829 g          417 t
ORIGIN      
        1 agcgtggcct cgtcggccca gtggacatcc tcgaagacgg cgatggtcgg gcgggcgcgc
       61 agtgtgtcga ggacggcggc gaagacggcc gcgcggtcgg tggggttggc cagcgccgcg
      121 gccagcgccg gcgcgccggc ggccatgtcg tagagagggc cgagcggccg gggtgtgaag
      181 agggcgtcgc acgcgcccca gagcaccgcc gcgccgtcgc cggtgctccg gtcgcctacc
      241 agacggccgg ccagggcgga tttgcccacg ccggcctcgc cgccgaccag cgccacctgg
      301 cctgggccgt ccctgaccgc gcgccaggcc cgttccagtg cgtccagggc cgcctcgcgc
      361 tcgaggagaa ggagtcgcat ggagctatca tacatcaggg ctgggttggg ggtaaagtgg
      421 ccgcggccct gagacgttat tcaggaccag tcaggtcccc atcgatcgta tggaaaggcc
      481 tcgaatcgcc tccctgtttc tgtgcggtat ggtaacattt gccagcctgg catttcattg
      541 gcgctctgga cgccctctca tctattggcc gggcagacac gccataaaca tcgacgttcg
      601 ccccaggcca acaaggagaa tccgcccatg tcccctcgct ccgcccacgc ccccctcaac
      661 ggcatcgact gcgcctacga aatggccggc gacggcccgc cggtcgtcct cctccacgcc
      721 ggcatcgccg acagccgcat gtgggcggcc cagttcgacg cgctggccac gaaccacact
      781 gtcatcgcct atggccggcg cggcttcggc cgcacccgcc ccgctcccgg ccccttcaac
      841 cacgtcgatg acctggccgc cctgctgggc tatctgggca tcaagcggac ggcgctggtg
      901 ggctgctccc agggcagtcg catcgccctc gacttcgccc tggcccatcc cgaccaggtg
      961 agccgcatcg tcctgagcgc gccgtccgtc agcggcttcc gctatgacgg cccgccaccg
     1021 cgacaggcgg acgcgatcga cgccgccgag gcggccggcg acctggcgcg ggtcaacgag
     1081 ttggagatgc agatctgggt cgatgggccg cggcgcgcgc ccgacgcggt ggcttcgtca
     1141 gtgcgccggc tagtggcgga gatgaacgcc atcgccctgg ccaacgaggg cgtgggcgag
     1201 gagcgcccgc ccgaccgccc ggccgccgga cggctaggcg aagtcggcgc gcccgttttg
     1261 ctcgtcgtcg gcgaactcga cgccgccata acgctggccg tggccgaggc gctggccggc
     1321 ggcctgccca acgtgcgccg cgtcgacctg ctcgccgccc acctgcccaa catggagcag
     1381 ccggaggcgt tcaatcgggc ggtggggccg tttctggggg aacgcgccta gcaccgggcc
     1441 ggccagcgcc ggccccccac ctcactgacg gctgcccttc tccgagaaca gaaaccccga
     1501 ctggcataca tgacgaatgt catgcccagg gcatgacatt cgtcactact tgcgttcggc
     1561 aactatctag tacgatagtc ctggacacag ccagtcgatt ctcgcgcacc ggagccagaa
     1621 gacgagtaag aaccgccgat tagggttgtt gtcttatctc gtttgtaggt cgctacatct
     1681 ctcgatttta acctcagccg cgacaatcaa actcctaact ggtctgtgga tcgtgccaaa
     1741 aggcggttgg gaggccgccg gcaatgaccc attcccctca ttcccctgtt atccgcgcgg
     1801 cgacggcgtt ggtgctgttc gccatcctag tcgtgggtat tggcgcgtgg agccaaccag
     1861 tcgccgccgc gcccacgtgt acgaccagtt gttacgtcag ccccacgggc agtgacgcca
     1921 acgacggcgc cacggcggcc acggccttca agacgatcca gaaggccatc gacacggtgc
     1981 aggccaacgg ccaggtgcga gtgctgcccg gcaactatag cgaaacggcc acaggccgcg
     2041 acgtgttggg cagcggcagc taccagttcg gcctcttctt cgaggagacg gccaagaacg
     2101 gcgtgacgct gcaaggcgtg accgcggccg atgtcaacat caccgacccc agcgccaccc
     2161 aggctgtcat caccaccaac gccaccaaca acttcggcta cagcggcgtc tttgttgagg
     2221 ccgacgatgt gaccatcgcc ggcgtcgaga ttggcgacaa cacgcccagc aacaacaaga
     2281 ccatcgaggt catcggcgat ggcttcacgt tcaagaactc gttcatgaac gtgtccgacg
     2341 gcggttcgct gtacttcggc gactggcgtt acaacgacac gaccgacgtg tcctacatcc
     2401 agagctacac cgtcgacaac aacatcttcg ccgacggctc cagcattgac atcgccagcg
     2461 gcgcgggcta caccggcccg gtggccggcc gcaccatcac caacaacgag ttttcgttct
     2521 cgttggccat gtatacggcg atgggcaacg tgtcctggcc gagcatcagc ttcaacggcg
     2581 acacgaccac cgtgtcgtgg tacacctacc ccgtgggcgg c
//
LOCUS       DAOSMQ010000462         2620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39187_length_2620_cov_4.814425, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000462 DAOSMQ010000000
VERSION     DAOSMQ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39187_length_2620_cov_4.814425"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PK829_12815"
     CDS             complement(<1..750)
                     /locus_tag="PK829_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOU42140.1"
                     /translation="MSKKIDAVVLISAAAEWRPVRAHFAAAPGEATPFGEAITVSIPV
                     AGRPRSVVLFHGGWGKIDAAASTQYAIDRWRPGLLLNLGTCGGFAGDVAAGTLLLANF
                     TLVYDIVEQMGDPDEALAHYAKEIDLSWLRGPLPLPVQRELLVSADRDIVSAEIADLR
                     ARFGAIAADWESGAIAHVAARNGVRCLILRGVTDLVSASAGEAYGRPELFVARTEALM
                     IRLLATLPEWLAAALPDDGGAILPNRALVLAR"
     gene            860..>2620
                     /locus_tag="PK829_12820"
     CDS             860..>2620
                     /locus_tag="PK829_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42141.1"
                     /translation="MRTLRVLALVLCAGFAALTVGCAIPEETAEVAPTTATPADAATI
                     PVEPTGAPAEPTTEPAAALPLAVAYDLGDATIVQANFPEDSRFRNMPIRLNGVIAAPA
                     GAGPYPVVVILHGTHPGCPVDEMGVDRWPCDPADEQANYSGFAYLASALAARGYVALA
                     PNINAENTFGFGEPVPGERLAQLLDLHLNALKAATAGGENAFGVELSGVADVGRVALI
                     GHSRGGEMASYLTRKYGLGTAESAAERGYGPVAGLLLVAPARILQDSAGADVPLAVIL
                     PACDGDVVGQDGQTFYEAVRFQTNHTWATTVLLEGANHNGFNTILGGDMIAHAGRPGC
                     QSPLAPEEQRQFLVDYAGDFLTTLFGAEDEAAAAKARLGLDLTQPAPGELFGRPARIN
                     SLSASADRLRLFDPGDETELMTNLQAGPTVAEGATLFFCPPGYTVEAELAACRRPNFV
                     NPANPAMAVISWSGPAEVRFEMPAGQFELGRHTTLTLRAALDPLSEQNAAGAPQSFSV
                     RLTDGSGATASVVAGPGEPALQYVTGEFTADETFPPGLLDAVVYLATVRVPLASFAGI
                     DLSDVDEVALVFDQTASGTLF"
BASE COUNT          408 a          926 c          905 g          381 t
ORIGIN      
        1 ccgcgccagc acaagagcgc gattcgggag aatcgcgcca ccgtcatcgg gcagggccgc
       61 ggccaaccac tccggcagcg tcgccagcag ccggatcatg agcgcctcgg tgcgcgcgac
      121 aaatagctct ggccggccat aggcttcgcc tgcgctggcg ctgaccaggt cggtgacgcc
      181 acgcaggatg aggcagcgca cgccgttgcg ggcggcgacg tgggcgatgg ccccgctctc
      241 ccaatcggcg gcgatggccc caaagcgggc gcgcagatcg gcgatttccg cgctgacgat
      301 gtcgcggtcg gccgagacca gcagttcgcg ctggacgggc agcggcagcg gcccgcgcag
      361 ccaggacagg tcgatctcct tggcgtaatg ggccagggct tcgtccgggt cgcccatctg
      421 ctccacgatg tcgtaaacca gggtgaagtt ggccagcagc agggtgcccg cggccacgtc
      481 gccggcgaag ccgccgcacg tgcccaggtt gagcagcagc cccggccgcc agcggtcgat
      541 ggcgtactgc gtcgaggccg cggcgtcgat cttgccccag ccgccgtgga agaggacgac
      601 cgagcgcggc cggccggcga cggggatact cacggtgatg gcctcgccaa agggcgtcgc
      661 ctcgcccggc gcggcggcga agtgggcgcg cacgggccgc cattcggccg cggcggagat
      721 cagtacaacc gcatcgattt tcttagacat tggcccatta taatggttgt ccgcgcccgt
      781 ttgctatact acgcggcgtc ggggcaagcc tcgcagacca caccggccca cggccctgct
      841 gcccccagag gagaatgaca tgcgaacgtt gagagtgctc gcgctggttc tatgcgcggg
      901 attcgcggcg ctgactgttg gctgcgccat ccccgaagag acggccgaag ttgcgcccac
      961 gaccgccacg ccggccgacg ctgccaccat cccggtcgaa ccgacgggcg ccccggccga
     1021 accgacgacc gaaccggcgg cggcactacc gctggccgtc gcctacgacc tgggcgatgc
     1081 gaccatcgtg caggccaact tccccgaaga cagccgcttc cgcaacatgc ccatccgcct
     1141 gaacggcgtc atcgctgcgc cggcgggcgc ggggccatac cctgtggtgg tcatcctcca
     1201 cggcacgcac cctggctgtc cagtggatga gatgggcgtc gaccgctggc cgtgcgaccc
     1261 ggccgatgag caggccaact actccggctt tgcctacctg gccagcgcat tggccgcgcg
     1321 cggctacgtg gccctggccc ccaacatcaa cgccgagaac acctttggct tcggcgagcc
     1381 tgtccccggc gagcgactgg cgcaactgct ggacctgcac ctgaacgcgc tcaaggcggc
     1441 caccgcgggc ggcgagaacg ccttcggcgt cgagttgagc ggcgtggccg acgtgggccg
     1501 ggtggcgctc atcggccatt cgcgtggcgg cgagatggcc agctacctga cgcgcaaata
     1561 tggcctgggc acggccgaaa gcgcggccga gcgcggctat ggcccggtgg ccgggctgtt
     1621 gctggttgcg ccggcgcgca tcctgcagga cagcgccggc gccgacgtac cgctggccgt
     1681 catcctgccc gcctgcgacg gcgatgtcgt cggccaggac ggccaaacct tctacgaagc
     1741 cgtgcgcttc cagactaacc atacctgggc gacgacagtg ctgctggagg gggccaatca
     1801 caacggtttc aacaccattc tgggcgggga tatgatcgcc catgccggcc ggcccggctg
     1861 ccaatcgccg ctcgcgcccg aggagcagcg gcaattcctc gttgattacg ctggcgactt
     1921 cctgacgacc ctcttcggcg cggaggacga ggcggcggcg gccaaagccc ggctgggcct
     1981 cgatctcacc cagccggccc cgggcgagct tttcggccgt ccggcgcgta tcaacagcct
     2041 gtcggcctcg gccgaccggc tgcggctgtt cgaccccggc gacgagacgg aactgatgac
     2101 caacctgcag gccgggccaa cggtggccga gggggcgacg ctgttcttct gtccgccagg
     2161 ctacaccgtt gaggcggagt tagccgcctg tcgccggcca aacttcgtca acccggccaa
     2221 cccggcaatg gcggtcatca gttggagcgg cccggccgaa gtgcgctttg agatgccggc
     2281 cgggcaattt gaattgggcc gccacacgac gctgacgctg cgcgccgcgc tcgacccgct
     2341 gtcggagcaa aacgccgccg gcgcgccgca gagcttcagc gtgcggctga ccgatgggag
     2401 cggggcgacg gccagcgtcg tcgccggccc cggtgagccg gctctccagt acgtcaccgg
     2461 cgaattcacc gcggacgaga ccttcccgcc cggcctgctg gatgccgtcg tctacctggc
     2521 aaccgtgcgc gtgccgctgg cgtccttcgc cgggattgac ctgagtgacg tggatgaggt
     2581 cgccctggtg ttcgaccaga cggcgagcgg cacgctgttc
//
LOCUS       DAOSMQ010000463         2619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39214_length_2619_cov_3.264821, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000463 DAOSMQ010000000
VERSION     DAOSMQ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39214_length_2619_cov_3.264821"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PK829_12825"
     CDS             <1..912
                     /locus_tag="PK829_12825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU42142.1"
                     /translation="RAPIATSAPATMAAPPTPTPQATASPMRRPPGTTATPTTTPVPT
                     FTPPPPPPPVAGEHLLWGRPVPASGPTWTDKAYPYGSTRGGTLRPHTGVEFVVPTGTP
                     VLAVAAGTVVVAGDDGQVAYGPQTNFYGNLVILELAGAADGVPIYALYGHLSVVDVSV
                     GQVVAAGEALGLSGASGVADGPHVHLEARVGENSYGATRNPLLWLRPLGNTGVVVGRV
                     AGSNAELLHEVPVVLRRIDGAAPFTATTSYATGEPNADGSLNENFVLDDVEPGFYEVT
                     AGDSARQATAELWVYPGRLNWVELVLP"
     gene            complement(884..1597)
                     /locus_tag="PK829_12830"
     CDS             complement(884..1597)
                     /locus_tag="PK829_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HOU42143.1"
                     /translation="MNNKALLTIVRWLVIVAMPFLLGLGMIRAVIAWDYPGFEYARIP
                     SDVFGFTNEQRVALAQGTLDYLRRPEPADEVIDVLLDLRLPGSDRPLYNEREVDHMID
                     VKNLTDAIKRLAYGAAAIVVVGLAILLIPRATRAYGWRTVFLAGLATVIALAVIALLI
                     LVAWPIFFVQFHELLFPPETWTFSYSDSLIRLFPEQFWFDFGVLVSVGTLVLGILVTL
                     LGWLMSRRRRATGAQARPS"
     gene            complement(1623..>2619)
                     /locus_tag="PK829_12835"
     CDS             complement(1623..>2619)
                     /locus_tag="PK829_12835"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOU42144.1"
                     /translation="GLIGVWQFGQTRRGSRAALLALPALLAPTVFFGLRGGVKSYADQ
                     VYEAESAYNYIQVIRQDGCNYLLLNEGQAYHSFYCDGGRVPAVSVWSTMLAAPYFNAG
                     AQPPQRVAMVGLAAGTMAKQFTRVFGPLPIDGIELDPAVVAAGRAYFALDEANIRVVI
                     GDGRYALAGLPGPYDVITVDAYKVPYIPWHLTTREFFAEAAGKLSDTGVLAINVGRVP
                     GDRRLVDAVGATLASVFPSVHAIDVPGTLNTIVVATRQPTTAANLAANLAVLPPAADA
                     LLRDALRTAVANPGVIAPGGTVFTDDRAPVETISDSVVVRYLLEIGPEGLNTLGQ"
BASE COUNT          474 a          906 c          842 g          397 t
ORIGIN      
        1 cgcgccccga ttgctaccag cgccccggcc acaatggccg ccccgcccac gcccacgccc
       61 caggcgaccg ccagccccat gcgccgcccg cctggcacaa ccgccacgcc tacgaccacg
      121 cccgtgccga ccttcactcc cccacccccg ccgccacctg tggccggcga acacctgcta
      181 tggggccggc cggttcccgc cagcggcccg acgtggactg acaaggccta cccctacggc
      241 agcacgcgcg gcgggacgct gcgcccgcat accggcgtgg agttcgtcgt gcccacgggc
      301 acgccagtcc tggccgtggc cgcgggcact gtggtagtcg ccggtgacga tggccaggtc
      361 gcctatggcc cgcagaccaa cttttacggc aacctggtca tcctggagtt ggccggcgcg
      421 gccgacggcg taccgatcta tgccctctat ggccacctgt cggttgtgga cgttagcgtt
      481 ggccaggtgg tggccgcggg cgaggcgttg ggcctttccg gagccagtgg cgtagccgat
      541 gggccgcacg tccatttgga ggcgcgcgtc ggcgagaata gctatggagc cacgcgcaac
      601 ccgctgctgt ggctgcggcc gttgggcaac acgggcgtcg tcgtcggacg tgtggccggt
      661 tctaacgccg agttgctaca cgaagttccg gtcgtgctgc ggcggatcga tggcgctgcc
      721 cccttcacgg ccacgacctc ttatgccacc ggcgagccta atgccgatgg cagcctgaac
      781 gagaacttcg tcctcgacga cgtcgagccg ggcttctatg aagtgaccgc cggcgacagc
      841 gcgcgccagg caacggccga gttgtgggtt tatcctggcc ggctcaactg ggtcgagctt
      901 gtgctcccgt agcccgccta cgccggctca tcaaccaacc cagcaacgtc accaggatgc
      961 ccagcaccag cgtgcccacg ctgaccagca cgccaaagtc aaaccagaac tgctccggga
     1021 acagccggat aagcgagtcg ctatacgaaa acgtccacgt ctccggcgga aagagcagtt
     1081 catgaaactg tacaaagaaa atcggccagg cgacgaggat cagcagggca atgacggcca
     1141 gggcaatgac cgtcgccagt ccggccagaa acaccgtccg ccagccataa gcgcgcgtcg
     1201 cccgcgggat cagcaggatg gccagtccga ccacgacgat cgcggcggct ccataggcca
     1261 ggcgcttgat ggcatcggtc aggttcttga cgtcgatcat gtgatcgacc tcgcgctcat
     1321 tgtaaagcgg ccggtcgctg cccggcagac gcaggtcgag taagacgtcg ataacctcgt
     1381 cggccggctc tggccgccgc aggtagtcga gcgtgccctg ggccagcgcc acccgctgct
     1441 cgttggtaaa gccaaacacg tccgacggaa tgcgcgcata ctcaaagccg ggatagtccc
     1501 aggcgatcac cgcgcggatc atgcccagcc ccaacagaaa tggcatggcc acgatgacca
     1561 gccagcgaac gattgttagc agtgctttgt tgttcaagct gtcctcataa gtgccggcgc
     1621 tctcactggc ccagggtatt cagcccttcc gggccaatct ccagcaagta gcgcaccacg
     1681 accgagtcgc taatggtctc cacgggggcg cggtcgtcag tgaagaccgt tccgccgggc
     1741 gcgatgacac ccgggttagc caccgccgtg cgcagcgcgt cgcgcaacag cgcgtcggcc
     1801 gcgggcggca acacagccag attggccgcc aggttggccg ccgtcgtcgg ctgtcgtgtt
     1861 gccaccacga ttgtattcaa tgtgcccggc acgtcgatag cgtggacgct gggaaacacg
     1921 ctggccaacg tcgccccgac ggcatcgacc agccgccggt cgccgggcac gcggccgacg
     1981 ttgatggcca gcacgcccgt gtcgctcagc ttgccggccg cctcggcaaa gaactcgcgc
     2041 gtagtcaggt gccaggggat gtagggtacc ttgtaggcat cgacggtgat gacatcatac
     2101 ggccccggca agccggccag ggcataacgg ccgtcgccga tgaccacgcg gatattggcc
     2161 tcgtcgaggg cgaaataggc tcgcccggcg gcgaccacgg ccgggtcgag ttcgatgcca
     2221 tcgatgggca gtggcccgaa aacgcgcgtg aactgcttgg ccatcgtccc cgcggccagg
     2281 ccgaccatgg ccacgcgctg cggcggctgc gcgccggcgt tgaaataggg cgcggccagc
     2341 atggtgctcc agaccgacac agccggcacg cggccgccgt cgcagtagaa ggagtggtaa
     2401 gcctggcctt cgttgagcag caggtagttg cagccgtcct ggcgaatgac ctggatgtag
     2461 ttgtaggccg attccgcctc gtagacctga tcggcgtagc tcttgacgcc gccgcgcaag
     2521 ccgaagaaga ccgtgggcgc aagcagcgcc ggcaaggcca gcaacgccgc cctcgagccg
     2581 cgccgcgtct gtccgaactg ccacacacca ataaggccc
//
LOCUS       DAOSMQ010000464         2610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39378_length_2610_cov_4.808219, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000464 DAOSMQ010000000
VERSION     DAOSMQ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39378_length_2610_cov_4.808219"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK829_12840"
     CDS             <1..358
                     /locus_tag="PK829_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42145.1"
                     /translation="LGLLLAACGGDNPPLAVDEAAKVVCSDDCVAHGQCGRLVDDRRV
                     VLANEGRPAVWAHNRFFLDETLVTVREVSQRELIAARDQVPLPETEATRFPHIFYRVE
                     GEGKNAWVSEWCVARP"
     gene            387..1226
                     /locus_tag="PK829_12845"
     CDS             387..1226
                     /locus_tag="PK829_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOU42146.1"
                     /translation="MTGEDRPVLEELLAAQARREPVALAVIIRDHGSVPRHAGTKMLV
                     YGDGRTLGTIGGGEMEARVVAAAAEALRDGRTRLVPYSLVDPQRGDPGVCGGQVEIYV
                     EPYVSLPTLYVLGCGHVGRALVSLGHWLGYRVVAWDDRPELATAGNVPDADARLSGPL
                     AEALATQPIDARTYVAVVTRNVGLDRELLPQLLATPAAFIGVMGSRRRWQETRRQLLA
                     DGLPAERLTGVNAPIGLEINAESPAEIAVSIIAQIIMAQRGGDGRPMAAPAAAPEEAT
                     SST"
     gene            1236..>2610
                     /locus_tag="PK829_12850"
     CDS             1236..>2610
                     /locus_tag="PK829_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU42147.1"
                     /translation="MFTHNVPPILDRYEMPADLASALELLARHGRAARVVAGGTDLLL
                     EMERGLRPDLKVIIDITRIPDLNSISRDADGQIHIGALVTHNQVVASTDLVAHALPLA
                     QACWEIASPQLRNRATVAGNLITASPANDTISALRALDAQVILASTRGWRMVRLADFY
                     TGVRRTVMEPDELLVEIVLAPLPETARGLFVKLGLRRAQAISVVHLAVVLDFAADGHT
                     VSDARITLGSVAPTIINATTAEDYLSGKTLSDDEITVAAGLAAAAATPIDDVRGPAAY
                     RAEMVEVMIRRALLALRDGRERENWPADPAMLWGDTGGLFPTGPDYAASFGPDTSIET
                     TVNGRPLSAPGISKTLLRWLREEGQLTGTKEGCAEGECGACTVYLDGMAVMACLVPAA
                     RAHHAEVVTVEGLATPAGLHPIQQAFIQTGAVQCGYCIPGFLMSGAKLLDEKPAPDPV
                     QIGQAFAG"
BASE COUNT          388 a          987 c          847 g          388 t
ORIGIN      
        1 cctcggtttg ctgctggctg cttgtggcgg cgacaacccg cccctggccg tggacgaggc
       61 ggccaaggtc gtctgtagcg atgactgcgt cgcccacggg caatgcggcc ggctggtgga
      121 cgaccgccgc gttgtgctgg ccaatgaggg ccggccggct gtctgggcgc acaaccgctt
      181 tttccttgac gaaaccctgg taaccgtccg cgaggtgagt cagcgcgagc tgatcgccgc
      241 gcgtgaccag gttccccttc ccgaaaccga ggccacgcgg tttccccaca tcttctaccg
      301 cgtggagggc gaaggcaaga acgcctgggt gtccgagtgg tgtgtggcgc ggccctgatt
      361 ccgacagctt tgccgaaaga taaaccatga ctggcgaaga tcgccctgtc ctcgaagaac
      421 tcctggccgc ccaggcccgg cgtgagccgg tagccctggc ggtcattatc cgcgaccacg
      481 gctctgtgcc gcgccacgcg ggcacgaaga tgctcgtcta cggcgacggc cgcaccctgg
      541 gcaccatcgg cggcggcgag atggaagccc gcgtcgtggc cgccgcggcc gaggcgctgc
      601 gcgacggccg cacccggttg gtgccctact ccctggtcga tccccagcgc ggtgaccccg
      661 gcgtctgtgg cggccaggtg gaaatctacg tcgaacccta tgtcagcttg ccaacgctgt
      721 acgtgctcgg ctgcggtcac gtcggccgcg ccctggtgtc gttgggccac tggctgggct
      781 accgcgtcgt cgcctgggac gaccgcccgg agctggccac ggccggcaac gtgcccgacg
      841 ccgacgcgcg cctgagcggc ccgctggccg aggccctggc aacccagccc atcgacgcgc
      901 gcacctatgt cgccgttgtc acccgcaacg tcggcctcga ccgcgagcta ttgccacaac
      961 tgctggccac gcccgccgcc ttcatcggcg tcatgggcag ccgccgccgc tggcaggaga
     1021 cgcgccgcca gctattggcc gacgggctgc ccgccgagcg tctgacgggc gtgaacgcgc
     1081 ccatcggcct ggaaatcaat gccgaatcgc cggccgaaat cgccgtcagc atcatcgccc
     1141 agatcatcat ggcccaacgc ggcggcgacg gccggcctat ggccgcgccg gccgccgcgc
     1201 cggaagaggc caccagttcc acctgacaac cgcctatgtt tacccacaac gttcctccca
     1261 tcctcgatcg ctacgagatg cccgccgatc tggcttccgc tctggagctt ctggcccgcc
     1321 acggccgcgc cgcccgcgtc gtcgccggcg gcacagacct gctgctggaa atggagcgcg
     1381 gcctccggcc cgacctcaag gtgatcatcg acatcacccg catccccgac ctcaactcca
     1441 tcagccgcga cgccgacggc caaatccaca tcggcgcgct ggtgacgcac aaccaggtcg
     1501 tcgcctcgac cgaccttgtc gcccacgccc tgccgctggc ccaggcctgc tgggaaatcg
     1561 cctcgcccca actgcgcaac cgggccacgg tcgccggcaa cctcatcacc gccagcccgg
     1621 ccaacgacac catctccgcc ctgcgcgccc tcgacgccca ggtcatcctg gcctcgacgc
     1681 gcggctggcg catggtgcgg ctggccgact tctataccgg cgtgcgccgg acggtcatgg
     1741 agccggatga gctgctggtc gagatcgtcc tcgcgccgct gccggagacg gcccgcggcc
     1801 tgttcgtcaa actgggactg cggcgggcgc aggccatctc cgtcgtccat ctggccgtcg
     1861 tgctcgactt cgccgccgac gggcacacgg ttagcgatgc ccgcatcacc ctggggagcg
     1921 tcgccccgac gatcatcaac gctaccacag cggaggacta tttgtccggc aagacgctga
     1981 gcgacgacga gatcaccgtc gccgccgggc tggccgccgc cgcggcgacg cccatcgacg
     2041 acgtgcgcgg cccggcggcc taccgcgccg agatggtcga ggtgatgatc cgccgggcgc
     2101 tgctggccct gcgcgacggc cgcgagcgcg agaactggcc ggccgacccg gccatgctct
     2161 ggggcgacac cggcggcctg ttccccaccg gccccgacta tgccgcctcc tttggccccg
     2221 acacgtccat cgagacgaca gttaacggcc ggccgctcag cgcgccgggc atctccaaga
     2281 cgctgttgcg ctggctgcgc gaggaagggc agctgacggg cacaaaagag ggctgcgccg
     2341 agggggagtg tggcgcgtgt accgtctacc tggacggcat ggccgtcatg gcctgccttg
     2401 tgcccgccgc ccgcgctcac cacgccgagg tcgtcactgt cgaagggctg gccacgccgg
     2461 cggggctcca ccccatccag caagccttta tccagaccgg cgcggtgcag tgcggctact
     2521 gcatccccgg cttcctcatg tccggggcca agctgctcga cgagaagccc gcgcccgacc
     2581 ccgtgcagat cggccaggcc tttgccggca
//
LOCUS       DAOSMQ010000465         2602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39550_length_2602_cov_4.639183, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000465 DAOSMQ010000000
VERSION     DAOSMQ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39550_length_2602_cov_4.639183"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PK829_12855"
     CDS             <1..1186
                     /locus_tag="PK829_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOU42148.1"
                     /translation="RCYSADPGLGGRDGLAIARAELAQRGMSLILDFTPNHVARDHDW
                     TTRRPELFIRGTADDLATAPDAFFAAGNAIIAHGRDPNFLPWRDTAQLNVFDSHLRAA
                     AIETLRDIAGQCDGIRCTSAMLVINEVFAGNWGQRADLWPDGEYWRAVLAAVRATHPG
                     ACFIAEVYWGREWELLQQGFDLCYDKQLYDRLVHETAETVRGHLTSDPAYQDRLVRFL
                     ENHAEPRAAAVFPASRALAVAIAAYTLPGAKMFHQGQFEGYATRVPAALSRRPDEPAD
                     RALGAFYRRLALSLQTTPLRDGQWRLCALNGWPDNYSYNNLVAWCWQAGDERRVIVVN
                     LSPHTCQARIQLPWPELAGQNWVLHDIMEDRIFEHGGDEILNIGLFVELAPWGRHFFA
                     FR"
     gene            1356..>2602
                     /locus_tag="PK829_12860"
     CDS             1356..>2602
                     /locus_tag="PK829_12860"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOU42149.1"
                     /translation="MTENLNPFAIAQAQLDKAAEILQLAPDMHAFLREPMREFHFTIP
                     VRMDDGHTQTFKAFRVQYNDARGPAKGGIRFHPDETIDTVRALSAWMTWKTAVADVPL
                     GGGKGGVICEPRRMSAGERERLSRGYMRVVARYVGLRQDVPAPDVNTNPEIMAWMLDE
                     YETIHGAHEPGVITGKPLTLGGSAGRSPATAMGGMVTIREAAKVLGMPLAGATCAIQG
                     YGNVGSFAHKLAHNLLGLKVTAVSDEFGAIYCEGGLDPEVVSAHMRRNGKVGGCPGSE
                     TISNAELLEMPVDILIPAAIENQLTSANADRIRARIVAELANGPTTPEADQVLTEKGI
                     YIIPDFLCNAGGVTVSYFEMVQNSYGFYWTEDLVQQRLDEKMTTAFHAVHEMATERQI
                     ETRMAAYLVAVNRVAEAVRARGWV"
BASE COUNT          437 a          932 c          838 g          395 t
ORIGIN      
        1 gcgctgctat agcgccgacc ccggcctggg cggccgcgac ggcctggcga tagcgcgggc
       61 cgagttggcc cagcgcggca tgagcctcat cctcgacttc acgcccaatc acgtcgcccg
      121 cgaccacgac tggacgacca gacggccaga gctgttcatc cggggcacgg ccgacgacct
      181 ggccaccgcg cccgacgcct tttttgccgc gggcaacgcc attatcgccc acggccgcga
      241 ccctaatttc ctcccctggc gcgacaccgc ccagttgaac gtcttcgaca gccacctgcg
      301 cgccgccgcc atcgagacgc tgcgcgacat cgccgggcaa tgcgacggca tccgctgcac
      361 gtcggccatg ctggtcatca acgaggtttt cgccggcaac tggggtcagc gtgccgacct
      421 gtggcccgat ggggagtact ggcgtgcggt tctggccgct gtccgcgcca cccaccctgg
      481 tgcctgcttc atcgccgagg tctactgggg ccgggagtgg gagttgctgc aacagggctt
      541 cgacctgtgt tatgacaagc aactctacga ccggctggtg cacgagacag ccgagacggt
      601 gcgcggccat ctgacttccg acccggccta tcaggaccgg ctggtgcgct tcctggaaaa
      661 ccatgccgag ccgcgcgccg cggccgtctt cccggccagc cgcgccctgg ctgtggccat
      721 cgccgcctac accctgccgg gggcgaagat gttccaccag ggacagttcg agggttacgc
      781 cacgcgcgtg cccgcggcgc tgtcgcgccg gcccgacgag ccagccgacc gggccctggg
      841 cgcgttctac cgtcgcctgg ccctgtcgct gcaaacgacg ccgctgcgcg acggccagtg
      901 gcgactttgc gcgctaaatg gttggccgga taattacagt tataacaacc tggtcgcctg
      961 gtgttggcag gcgggggacg aacggcgcgt catcgtcgtc aacctgtcgc cccatacctg
     1021 ccaggcacgc atccagttgc cgtggccgga actggccggt cagaactggg tgctgcacga
     1081 catcatggaa gaccgtatct tcgagcacgg cggcgatgag atcctcaaca tcggtctctt
     1141 cgtcgagctg gccccctggg ggcggcactt cttcgctttc cgctgacagc ggcgcggtgc
     1201 gcgtccgccg cgcaccgcgc ctcgctcatt acgaatagaa tcacctcgcc agtcgcccac
     1261 ggcgcgctgg cgtttccatt tctgaagcaa gcgaccgccg gcccgccccg cgcgccggca
     1321 acacgttccc aaatcccaca ggagtcacag acaacatgac cgagaacctg aacccgtttg
     1381 ccatcgccca ggcccagctg gacaaggccg ccgaaatcct gcaactggcc cccgacatgc
     1441 acgctttcct gcgcgagccg atgcgtgagt tccacttcac catccccgtg cgcatggacg
     1501 acggccacac ccagaccttc aaggccttcc gcgtgcagta taacgacgcc cgcggcccgg
     1561 ccaagggcgg catccgcttc caccccgacg agaccatcga cacggtgcgc gccctgtcgg
     1621 cgtggatgac gtggaagaca gccgtggccg acgtgcctct gggcggcggc aagggcggcg
     1681 tcatctgcga gccgcgccgg atgtcggccg gggagcgcga gcgcctcagc cgcggctaca
     1741 tgcgcgtcgt cgcccgctac gtcggcctgc gccaggacgt gcccgcgccc gatgtcaaca
     1801 ccaacccgga gatcatggcc tggatgctgg acgagtacga gaccatccac ggcgcgcacg
     1861 agccgggcgt catcaccggc aagccgctga cattaggcgg ctcggccgga cgcagcccgg
     1921 ccacggcaat gggcggcatg gtcaccattc gcgaggcggc caaggtgctg ggtatgcctc
     1981 tggcgggggc cacctgcgcc atccagggct atggcaacgt cggcagcttt gcccacaagc
     2041 tggcccacaa cctgctgggg ctgaaggtga cggccgtcag cgacgagttc ggggcgatct
     2101 attgcgaggg cgggctggac ccggaagtcg tctcggccca catgcgccgc aatggcaagg
     2161 tcggcggttg ccccggcagc gagacgatca gcaacgccga gctgttggag atgcccgtcg
     2221 atatcctcat cccggcggcc atcgagaacc agctgaccag cgccaacgcc gaccgcatcc
     2281 gcgcccgcat cgtcgccgag ctggccaacg gcccgacgac gccggaggcc gaccaggtgc
     2341 tgaccgagaa gggcatctac atcatccccg acttcctgtg caacgccggc ggcgtcactg
     2401 tgtcgtactt cgagatggtg cagaacagct acggcttcta ctggaccgaa gacctggtgc
     2461 agcagcggct ggacgagaag atgaccaccg ccttccacgc cgtccacgag atggccacgg
     2521 agcgccagat cgagacgcgc atggccgcct acctagtggc cgtcaaccgc gtggctgagg
     2581 ccgtgcgggc gcgcggctgg gt
//
LOCUS       DAOSMQ010000466         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39767_length_2591_cov_4.652603, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000466 DAOSMQ010000000
VERSION     DAOSMQ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39767_length_2591_cov_4.652603"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PK829_12865"
     CDS             <1..693
                     /locus_tag="PK829_12865"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU42150.1"
                     /translation="LVQRAPARQRPETNLFRHGVRQVVTTLGSLWREYPMTLRYLIAY
                     LIYNDGIQTVIVVAAAFATSELGVDAQTVLLLVLMIQFVAFGGALLFGRLAERMGAKR
                     AIMLSLVIWSGVVIYAYAFLANTTQLFVLGVPLALVLGGSQALSRSLFSQMIPDDHEA
                     EYFGFYEISERGTSWIGTFAFALAVQATGSQRIAIVALIIFFIVGLILLSRVDVRRAI
                     VEAGNDPSGVVL"
     gene            697..1500
                     /locus_tag="PK829_12870"
     CDS             697..1500
                     /locus_tag="PK829_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42151.1"
                     /translation="MTPSDLENVLAYVRERGTATELARLRYLLSGESAPADVRESFER
                     AQRSDGGWSPPWAPDYSSVDATCFQLALADQLGLDRRSPLFIDALRFLAERQRADGYW
                     EEEEEMAAAAPPWAQPGDTAARLYVTANCGFWAAVTGLVPTAARDAAAYLSYHLYEDG
                     ELPSFLQTHWLSAALWQQRGLVDEANKTLSYLAARVGDLSAGNLAWMIIALREGGVAA
                     ADAAISGALDRLLALRLPEGHWPSDEGIENSVHVTIEALKALQLCGRLP"
     gene            complement(1562..1969)
                     /locus_tag="PK829_12875"
     CDS             complement(1562..1969)
                     /locus_tag="PK829_12875"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOU42152.1"
                     /translation="MRKVLFLCTGNSCRSQMAEAIVNARLGDRWQAVSAGTIPSGYVH
                     PKAIAALAEIGIDHAGRSKTADEFRDVDFDLVVTVCDSAAEECPVWLGRGVRRHHSFP
                     DPAKTDDMADFRAVRDAIDREIVALLQAHDAAS"
     gene            complement(1980..>2591)
                     /locus_tag="PK829_12880"
     CDS             complement(1980..>2591)
                     /locus_tag="PK829_12880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU42153.1"
                     /translation="TALSAADCFSGASEAELSAEGRRQANCLAQRLAALPIVAFYCSP
                     LGRARATATIVAAPHALTPVVVPDLRELDYGQWETLPREEVTARFAADYAAWGVDPLA
                     SAPPGGETGLDVLARVLPALRAIVQRHTGQSVAVVAHKAANRLALCGLLGIDPRSYRD
                     RLDQSPACLNIVDFDADGQARLALFNDVSHYARGANESRAVGE"
BASE COUNT          387 a          884 c          894 g          426 t
ORIGIN      
        1 ctggtgcagc gcgcccccgc ccggcaacgg ccggagacga acctattccg ccacggcgtg
       61 cgacaggtcg tgacgacgct gggcagcctg tggcgcgaat atcccatgac cctgcgctac
      121 ctgattgcct acctcatcta caacgacggc atccagacgg tcatcgtcgt cgccgcggcc
      181 ttcgccacca gcgagctggg cgtcgacgcc cagacggtgc tcctgctggt gttgatgatc
      241 cagttcgtgg cctttggtgg ggcgctgctg ttcggccgat tggccgagcg catgggcgcg
      301 aagcgggcga tcatgctcag cctggtcatc tggtcgggcg tcgtcatcta tgcctacgcc
      361 ttcctggcca acaccacaca gcttttcgtc ctcggcgtgc cgttggcgct ggtgctgggc
      421 gggtcgcagg cgctcagccg gtcgctgttt tcgcagatga tccccgacga ccacgaagcc
      481 gagtacttcg gcttttacga gatcagcgag cgaggcacgt cgtggatcgg cacgtttgct
      541 ttcgctctgg cagtacaggc gactgggtcg cagcgtatcg ccatcgtcgc cctgatcatc
      601 ttcttcattg tcggtctgat cttgctaagc cgtgtggacg tgcgccgggc cattgtcgag
      661 gccggcaacg accccagcgg cgtcgtccta tagcctatga ccccttccga cctggaaaac
      721 gtcctggcct atgtgcgtga gcgcggcacg gccaccgaac tggcgcgctt gcgctacctg
      781 ctgagcggcg agagcgcccc ggccgacgtg cgcgagagct tcgagcgcgc ccaacgcagt
      841 gacggcggct ggtcgccgcc ctgggcgccc gactatagct ccgtcgatgc cacctgcttc
      901 caactggccc tggccgacca gttgggcctc gaccgccgtt cgccgctgtt catcgatgcc
      961 ctgcgcttcc tggcggagcg gcagcgcgcc gacggctact gggaagagga ggaggagatg
     1021 gccgcggccg cgccgccctg ggcccagccg ggcgacaccg ccgcgcgcct ctacgtgacg
     1081 gccaactgtg gcttctgggc ggcggtgacg ggcctggtgc ccacggccgc ccgcgacgcc
     1141 gccgcctatc tgagctatca cctgtacgag gatggcgagc tgccgtcgtt cctacaaacc
     1201 cactggctgt cggcggctct atggcagcag cgcggcctgg tggatgaggc caacaagaca
     1261 ctcagctatc tggcggcgcg ggtgggcgac ctctcggccg gcaacctggc ctggatgatc
     1321 atcgccctgc gcgagggcgg cgtggcggcg gccgacgcgg ccatcagcgg cgcgctcgac
     1381 cggctgctgg cgctgcgcct gccggagggg cactggccca gcgatgaggg cattgagaac
     1441 agcgtccacg tcaccatcga agcgctgaag gcgttgcaac tgtgcggtcg gctgccatga
     1501 ggccgggcga cgtcatctgg atgcgcgtct tcaaggccga cggccgcacc caccgcagcg
     1561 gttagctggc agcgtcgtgc gcttgcaaga gggcgacgat ctcccggtcg atggcgtcgc
     1621 gcacggcgcg aaagtcggcc atgtcgtcgg tcttggccgg gtcggggaag ctgtggtgcc
     1681 ggcgcacgcc gcgccccagc cagacggggc actcctcggc cgccgaatcg cacactgtca
     1741 ccaccagatc gaagtccaca tcgcggaact cgtcagccgt cttcgaccgg ccggcgtggt
     1801 cgatgccgat ctcggccagg gcggcgatgg ccttggggtg gacgtaaccg ctgggaatcg
     1861 tgccggcgct gacggcctgc cagcggtcgc ccaggcgggc gttgacgatg gcctccgcca
     1921 tctgcgagcg gcaagagttg ccggtgcaga ggaataatac tttgcgcaag gatgccctcc
     1981 tattctccta ccgcgcgaga ctcattcgcg ccgcgggcgt aatgtgacac gtcattgaac
     2041 agcgccagcc gcgcctggcc gtctgcgtcg aagtcaacga tgttgaggca ggccggcgac
     2101 tggtcgagcc ggtcgcgata gctgcgcggg tcgatgccca gcagaccaca gagcgccagc
     2161 cggttggctg ccttgtgggc cacaacggcc acggattgcc ccgtatgacg ctgcacaatg
     2221 gccctcagcg ccggcagcac gcgcgccagc acgtccagac ccgtctcgcc gcccggcggc
     2281 gcgctggcca gcgggtcaac gccccaggca gcgtagtcgg cggcgaaacg cgccgtaacc
     2341 tcctcgcggg gcagcgtctc ccactggccg taatcgagtt cacgcaggtc gggcacgacc
     2401 accggcgtca gcgcgtgcgg cgcggcgacg atggtcgctg tcgccctggc gcggccgagg
     2461 gggctgcaat agaaggcgac gatgggcagc gcggccagtc gttgggccag gcagttggcc
     2521 tgccgccggc cttcggccga cagctcggcc tcgctggctc cggaaaaaca atcggcggcg
     2581 ctcagggcgg t
//
LOCUS       DAOSMQ010000467         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39774_length_2591_cov_3.373423, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000467 DAOSMQ010000000
VERSION     DAOSMQ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39774_length_2591_cov_3.373423"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK829_12885"
     CDS             <1..611
                     /locus_tag="PK829_12885"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOU42154.1"
                     /translation="NGRLRHMPWADLRAIDSDMLEQTFTLRGDHSKIRVSLTMNGIAW
                     LFDTVHLARPDLWRKSDRRVFRFRPIWAILSLAFSAGWWAFLIVAAIYGPGGALWQWL
                     LLAALGLAPLAILLRRPYRLQIEGDALRVRYLRREEIIPATAIAHIWTKRAGTVAISN
                     LALELHLVDGRRLSLGGFEGGTGELANTLMAWWQRARADEPQ"
     gene            complement(650..1639)
                     /locus_tag="PK829_12890"
     CDS             complement(650..1639)
                     /locus_tag="PK829_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOU42155.1"
                     /translation="MKRRLLLTVGLPLLVVVLLAVLVVGLLQSAEVDALQLAQSVLGA
                     RAVPAWVAEWYGWLRLPLRLLFAVAIFLPAWLVTKISGRLAGWLMRVARYDGGDREAG
                     DQGVAGAPLMAHERRYETIRLLLASLINISAFVTAFILAAGQFVSLVNLAVISTILAN
                     AFGFAARDYIGDLLNGVSNIFEDRFDVGDNVAVFRPGDPVEGIVERVTVRTLSIRPRA
                     GELIIVPQGEVRILRNYSRGSFTGTVVTVRVAAAALPRAMAVLTPLTAEAPAVLPDLL
                     EPWRIVAPDGQLGSTAELLIHARARYGHGVEARLQIMALIEERLAAAGVQLTS"
     gene            complement(1636..>2591)
                     /locus_tag="PK829_12895"
     CDS             complement(1636..>2591)
                     /locus_tag="PK829_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOU42156.1"
                     /translation="AVGLAFYAQTNPAALYVITAAPTSPQAERSLYLLARIVLFPFQI
                     IFVFMTFSAALWIFRRRQRLAAWLLGEPLSGRFTVANDADLPFVLRPQRRRTRTQIVS
                     SIIAVVAILVAIVLALGQIVPRAELAVVIAALTSGLTWGARLPVGDVLGGLTNIVETT
                     VTVGERISYRQLSERVEGVVEGMDLRFINVRADSGELTTIPHGELRIFRNYSRGAQTG
                     VHATFPIAARDLGRAVALLGELATAAADLVPELIGPWQVMSLDGKLGPVIDLSVYGRT
                     TPGNETELHLAIHALVLERLAAAGIAVGAGADMTGATEAAE"
BASE COUNT          419 a          871 c          943 g          358 t
ORIGIN      
        1 tgaatggccg tctgcgccac atgccctggg ccgacttgcg cgcgattgac agcgatatgc
       61 tcgaacagac gttcactctg cgcggcgacc acagcaagat tcgcgtctca ctgacgatga
      121 acggcatcgc ctggctcttc gacactgtcc acctggcgcg gcccgaccta tggcgcaaat
      181 cagacaggag agtgtttcgc ttccgtccta tctgggccat cctgtcgctg gcattttcgg
      241 ccggttggtg ggcctttctg atcgtggccg ccatctacgg gccgggtggg gcgttgtggc
      301 agtggttgtt gctggctgcg ctggggctcg cgccgctcgc catcctgctg cggcggccct
      361 accgcttgca gatcgaagga gatgcgctgc gcgtgcgcta cctgcggcgg gaggaaatca
      421 tccccgcgac cgcaatcgct cacatctgga cgaagcgggc cgggacggtg gcaatctcca
      481 atctggctct tgaactgcat ctggtggacg gccgccggct gagcctgggc ggcttcgagg
      541 ggggcacggg cgagctggcc aacacgctca tggcctggtg gcaacgggcg cgggccgacg
      601 agccgcagta gccggccaac ccaccgcgcc aacgccggcg gccgcggcct cagctggtta
      661 gctgcacccc cgccgccgcc aaccgttcct caatcaacgc catgatctgc aaccgcgcct
      721 cgacgccgtg gccgtagcgg gcgcgggcgt ggatgagtag ctcggccgtg ctgcccagct
      781 ggccgtccgg ggcgacgata cgccacggct ccagcaggtc gggcagcacc gccggggcct
      841 cggccgtcag cggcgtcagc acggccatcg cccggggcag ggcggccgcg gccacgcgca
      901 cggtgacgac cgtgccggtg aagctgccgc ggctgtagtt gcgcaggatg cgtacctccc
      961 cctggggcac gatgatcagt tcgccggcgc gcgggcggat gctgagcgtg cgcaccgtca
     1021 cccgttcgac aatgccctcg actggatcgc cggggcggaa cacggccacg ttgtcgccca
     1081 cgtcaaagcg atcctcgaag atgttgctga cgccgttgag caggtcgccg atgtagtcgc
     1141 gggcggcgaa gccgaaggcg ttggccagga tggtggagat gacggccagg ttgaccaggc
     1201 tgacgaactg ccccgcggcc agaatgaagg cggtgacgaa ggcggagatg ttgatcaggc
     1261 tggccagcag caggcggatg gtctcgtagc ggcgctcgtg ggccatgagc ggcgcgccgg
     1321 ccacgccctg gtcgccggcc tcgcggtcgc cgccatcata gcgggcgacg cgcatcagcc
     1381 agcccgccag ccggccgctg atcttcgtga ccagccaggc tggcaggaag atggcgacgg
     1441 caaagaggag gcgcagcggc agccgcagcc agccgtacca ctcggccacc cacgccggca
     1501 cggcccgcgc gcccagcacc gactgggcca gttgcagcgc gtccacctcg gccgactgca
     1561 atagcccgac gaccagcacc gccagcagga cgacgaccag cagcggcagg ccaacggtca
     1621 gcagcaaacg gcgcttcatt cggccgcctc cgtcgccccg gtcatatccg cgccggcccc
     1681 gacggcaatg cccgccgcgg ccagccgctc cagcaccagg gcgtggatgg ccaggtgcag
     1741 ctcggtctcg ttgcccggcg tcgtgcggcc atagacggac agatcgatga cggggccgag
     1801 cttgccgtcg agcgacatca cctgccacgg gccgatcagc tccggcacga ggtcggccgc
     1861 cgcggtggcc agctcgccca gcagcgccac cgcccggccc aggtcgcgcg cggcgatggg
     1921 gaaggtggcg tggacgccgg tctgcgcgcc gcggctgtag ttgcggaaga tgcgtagttc
     1981 gccgtggggg atggtcgtca gctcgccgga gtcggcgcgg acgttgatga agcgcagatc
     2041 catgccctcg acgacgccct cgacgcgctc gctgagctgg cggtagctga tgcgctcgcc
     2101 cacggtgacg gtcgtctcga cgatgttggt caggccgccg agtacgtcgc ccacgggcag
     2161 gcgcgcgccc caggtcaggc cgctggtcag cgcggcaatg accacggcca actcagcgcg
     2221 ggggacgatc tggcccaggg ccaggacgat ggccacgagg atggccacga cggcgatgat
     2281 gctggagacg atctgcgtgc gcgtgcggcg gcgctgcggg cgcaggacga aaggcaggtc
     2341 ggcgtcgttc gcgacggtga agcgccccga cagcggctcg cccagcagcc acgcggcgag
     2401 gcgctgtcgc cggcgaaaaa tccacagggc ggccgagaag gtcatgaaga cgaagatgat
     2461 ctggaagggg aacaggacaa tgcgcgccag caggtagagc gagcgctcgg cctgcggcga
     2521 ggtgggagcg gcggtgatga cgtagagcgc cgccgggttg gtctgggcat agaaggccag
     2581 gccgacggcc a
//
LOCUS       DAOSMQ010000468         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39796_length_2590_cov_4.447337, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000468 DAOSMQ010000000
VERSION     DAOSMQ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39796_length_2590_cov_4.447337"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PK829_12900"
     CDS             <1..863
                     /locus_tag="PK829_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU42157.1"
                     /translation="WNWATMGQSVRPLTNMTHGGIPRETFVDAELIRYPTFDGRAIPA
                     WLYRPRTAAGPVPVVVNVHGGPEWQARPWFDFLTQYLVACGYAVLVPNVRGSTGYGNA
                     YSHLDDIGKRLDSVNDLAHAAHWLRAQADFDGRRVAVLGSSYGGYMVLAALAFHPELW
                     AAGVDIVGIANFVTYLENTSGYRRQHRESEYGRLDTDRELLERVSPINAVERMTAPLL
                     VIHGANDPRVPLSEAQQLVARLAALGRAAELLVFDDEGHGITLLKNKRVAYSAIARFL
                     NETLGASPED"
     gene            875..1210
                     /locus_tag="PK829_12905"
     CDS             875..1210
                     /locus_tag="PK829_12905"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOU42158.1"
                     /translation="MLVMYVAFGLLLAGLAVPLWLGKVPPNGWYGFRVPSTLYNEQLW
                     YKVNRYAARWMALAGVVTVVAALALYRVPGLTVDRYAWLLLAVFVLSFVPGLVLSFRY
                     LRRLQKSGV"
     gene            complement(1207..1758)
                     /locus_tag="PK829_12910"
     CDS             complement(1207..1758)
                     /locus_tag="PK829_12910"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOU42159.1"
                     /translation="MLRSKRVTLRAIERDDTPAIYAINNDVDVELAGGGDPPWPQSLA
                     RAYADFDRRAGEGGREGNPSGVSFAIEADGHLIGFCGLNYRDPFAATAELGITIGDKG
                     YWGQHYGREAIGLLLDYGFRLLNLRRIYLRVHARNERGVRAYRACGFVEEGRLRQHVW
                     SNGAYDDLLYMGVLRDEWTPPAI"
     gene            complement(1816..>2590)
                     /locus_tag="PK829_12915"
     CDS             complement(1816..>2590)
                     /locus_tag="PK829_12915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU42160.1"
                     /translation="DAGARFIVIGGGFIGSEVAASLRKRGNDVTLVFPEAGISDRLFP
                     AEMAAFLNDYYREQGVEVLSGESVTDMAADADGATVTLGSGRTLAADRVVAGIGIVPD
                     VALARAAGLASDNGILVDENLQTTQPDIYAAGDVANYPDLILGGRRRVEHADAARAMG
                     RAAGLNMAGAGQPYRYLPLFYSDLFALGYEAVGQCDSRLDIVADWGEAFRHGVLYYLR
                     DGRVRGVLLMDVWDKVDEARAIIEAGDVVAAADLRGRIR"
BASE COUNT          404 a          894 c          852 g          440 t
ORIGIN      
        1 tgtggaattg ggcgacgatg ggccagagtg tgcgcccgct gacgaacatg acccacggcg
       61 gcattccgcg ggaaacgttc gtcgatgctg aactgatccg ctatccgacg ttcgacggcc
      121 gcgccatccc cgcctggctc taccggccgc gcacggccgc cgggccggtt ccggtggttg
      181 tcaacgtcca cggcgggccg gagtggcagg cgcggccgtg gtttgacttt ctgacccagt
      241 acctggtcgc ctgcggctat gccgtcctcg tgcccaacgt gcgcggctcg accggctacg
      301 gcaacgccta tagccacctg gacgacatcg gcaagcgcct cgacagcgtc aacgacctgg
      361 cccacgccgc ccactggctg cgggcgcagg cggacttcga cggccggcgc gtcgccgtgc
      421 tcggcagcag ctacggcggc tatatggttc tggcggcgct ggccttccat cccgaactgt
      481 gggccgccgg agtcgatatc gtcggcattg ccaacttcgt cacctacctg gagaacacca
      541 gcggctaccg ccgccaacac cgcgagtcgg agtacggccg cctcgacact gaccgggaac
      601 tactggagcg cgtctcgccc atcaacgccg tcgagcgcat gaccgcgccg ctgctggtta
      661 tccacggggc caacgatccg cgcgtgcccc tcagcgaggc gcagcagctc gtcgcccgtc
      721 tggccgcgct cggccgggcc gcggagttgc tggtcttcga cgacgagggg catggcatca
      781 cgctgctgaa gaataagcgc gtcgcctaca gcgccatcgc ccgcttcctg aatgagacgt
      841 tgggcgcgtc gccggaggac tgaccataaa gacgatgctg gtgatgtacg tggccttcgg
      901 gctgttgctg gccgggctgg ccgtgccgct gtggctgggc aaggttccac ccaacggctg
      961 gtatggcttc cgcgtgccca gcacgctcta taacgagcag ctgtggtata aggtcaaccg
     1021 ctacgccgcg cggtggatgg ctctggccgg cgtcgtcacc gtcgtagccg cgctggccct
     1081 ctatcgcgtg cccggtctga cggtcgatcg ctatgcctgg ctgctgctgg cggtgttcgt
     1141 cctgtcgttc gttcccggcc ttgtgctcag ctttcgctat ttgcggcggt tgcagaagag
     1201 cggggtctag atggccggtg gcgtccattc gtcgcgcagc acgcccatgt agagcaggtc
     1261 gtcgtaagcg ccattgctcc acacgtgctg tcgcagccgc ccctcctcca caaaaccaca
     1321 ggcgcgatag gcgcgcacgc cccgctcgtt gcgggcgtgg acgcgcaggt agatgcgccg
     1381 caggttgagc aaccggaagc catagtcgag cagcagcccg atcgcctcgc ggccatagtg
     1441 ctggccccaa tagcccttat cgccgatggt gatgcccagc tccgctgttg ccgcgaaagg
     1501 gtcgcggtag ttcaggccac agaagccgat caagtggcca tcggcctcga tggcaaagga
     1561 gacgccggac ggattgccct cgcggccacc ttcgccggcg cgccggtcga agtcggcata
     1621 agcgcgggcc agcgattgtg gccacggtgg atcgccgccg ccggccaatt ccacgtccac
     1681 atcgttgttg atcgcataga tggccggggt atcgtcgcgc tcaatggcgc ggagagtcac
     1741 gcgttttgat ctcagcattg ggtcttactc ttctaaaggg atgacctgaa cagcccggct
     1801 cgcgccgatg ttggcctatc ggatgcgccc gcgaagatcg gccgcggcga ccacatcgcc
     1861 tgcctcgatg atcgcgcgcg cctcatcgac cttgtcccac acgtccatga gcaacacgcc
     1921 gcgcacgcgg ccgtcgcgca ggtagtagag cacgccgtgc cggaacgcct cgccccagtc
     1981 ggcgacgatg tccagacggc tgtcgcattg gccgacggcc tcgtagccca gcgcgaacag
     2041 gtcagagtag aacagcggca ggtagcggta gggctggccc gcgccggcca tattcagccc
     2101 cgctgcccgg cccatcgccc gcgcggcgtc ggcgtgttcc acacggcggc gaccgcccag
     2161 aatgaggtcg gggtagttgg ctacgtcgcc ggcggcgtag atatccggct gtgtcgtctg
     2221 caagttctcg tcgacgagga tgccattgtc gctggccagg ccagccgccc gcgccagagc
     2281 cacgtcgggc acaatgccga tgccggccac gacccggtcg gccgccagcg tccgcccgct
     2341 gcccagtgtc actgtggccc cgtccgcgtc cgcggccatg tcggtcacgc tctcgccgga
     2401 gagcacctcg acgccctgct cacggtagta gtcgttgagg aaagcggcca tttcggccgg
     2461 gaaaaggcgg tcgctgatgc ccgcctccgg aaagaccagc gtcacgtcgt tgccccgctt
     2521 gcgcagcgag gcagccacct ctgagccgat gaagccgccg ccgatgacga tgaagcgcgc
     2581 ccccgcgtcg
//
LOCUS       DAOSMQ010000469         2588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39834_length_2588_cov_4.172523, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000469 DAOSMQ010000000
VERSION     DAOSMQ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39834_length_2588_cov_4.172523"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PK829_12920"
     CDS             complement(<1..520)
                     /locus_tag="PK829_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU42161.1"
                     /translation="MEPVRLDLPADLGIGQVNAYLFTQPEVVLVDAGIKTDACWEALV
                     DGLALHGIAPADIARVVITHTHVDHYGLAGRLVAESDATVWVSELGAPWLSAAEALWH
                     ERLVYYRDDFLRHLGLPADVVAVTLHGLELLATRIDPVPAERIVPFHLDGVLQMGGQA
                     WQVIHTPGHASMQ"
     gene            complement(642..>2588)
                     /locus_tag="PK829_12925"
     CDS             complement(642..>2588)
                     /locus_tag="PK829_12925"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOU42162.1"
                     /translation="RGLALEKHRAATRGLDRSLSISDDLAPVSPALAEAVRAYGELNA
                     HLAYLRDRYPNETYGLHTAQLGTDLAEASAGDMVGRLRGKPAPPLRMRRQENLLAPLE
                     LMARSLREGGMADIRRAGLDRTLSQARIFGLHAAQLDLRQYSEYNTSVLDELLGRLDW
                     CAGFGALDGPGRAAVLTDALTRPAPDLSSFDHLSAEARETLDLFAVIKRAVDYYGPEL
                     LGPYIVSMTHGPEDILAPLLLARWHGLSPGEDGAEGLTFAPLFETRDDLRAAPDIMAA
                     LFDHPGYAPHLERVGRRQTIMIGYSDSNKDAGYLAANWELYQAQEALAAVCHGHDVVM
                     TLFHGRGGTIARGGGPANRAILAQPPGSVGGRIRITEQGEVISDRYSHPAIAERHLEQ
                     VVHAVLIASAPEHYRLQVRPQPEWRAAMDELAALSYRAYRQLIYETPELLVYWGHATP
                     MDEISQMRIGSRPSRRTAAATFTSLRAIPWGFSWMQCRHVLPGWFGVGAALDGFGRDP
                     EATSLLRQMYRDWPFFRNLLDNSQVSMAQADMGIARLYADLVPDGRVRELIYGQVATA
                     FEATRVAVLNVTGQRELLDHDPVLQRAVRRRNPYIDPLNFIQVSLLRRLRALPDADSA
                     EAEHIRNTIFLTINGIASGLKNTG"
BASE COUNT          388 a          820 c          985 g          395 t
ORIGIN      
        1 tctgcatgct ggcgtggccc ggcgtgtgga tgacctgcca cgcctgcccg cccatttgca
       61 gcacgccgtc gaggtgaaag ggcactatac gctcggcggg tacggggtcg atgcgcgtgg
      121 ccagcagctc caggccgtgg agcgtcacgg cgacgacgtc ggccggcaag cccagatggc
      181 gcaggaagtc gtcgcgatag tagaccaggc gctcatgcca cagcgcttcg gcggccgaca
      241 gccacggcgc gcccagctcc gacacccaca cggtggcgtc gctctcggcc accagccgcc
      301 cggccaggcc atagtgatcg acgtgggtgt gggtgatgac gacgcgggcg atgtcggccg
      361 gggcaatgcc gtgcagagcc aggccatcga ccagcgcctc ccaacaggcg tcggtcttaa
      421 tgccggcatc aacgaggacg acctccggct gggtgaatag ataggcgttg acctggccaa
      481 tgcccaggtc ggcgggcaaa tcgaggcgca caggctccat gcccctatta tgcgtgatct
      541 atgattggtg ggccatctcg cctcagttcc aaagtcatct gcgcccacct gtcaggtcgg
      601 tgcgcctggg gtgactttgg aaaagctctg tatctcgccc gctagccggt gttcttgagg
      661 ccggaggcga tgccattgat ggtcaggaag atagtgttgc ggatgtgctc ggcctcggcg
      721 ctgtcggcgt cgggcagggc gcgcaggcga cgcaagaggc tgacctggat gaagttcagc
      781 gggtcgatgt aggggttgcg ccggcggacg gcgcgctgca agaccgggtc gtggtcgagt
      841 agctcgcgct ggccggtgac gttgaggacg gcgacacggg tggcctcgaa ggcggtggcc
      901 acctggccat agatgagttc gcgcacgcgc ccgtcgggca ccaggtcggc gtagagccgg
      961 gcgatgccca tgtcggcctg agccatggag acctgggagt tgtccagcag gttgcggaag
     1021 aagggccagt cgcggtacat ctgccgcagc agcgatgtcg cctccgggtc gcggccgaag
     1081 ccgtccagcg ccgcgccaac gccaaaccag ccgggcagga cgtggcggca ctgcatccag
     1141 ctaaagcccc aggggatggc ccgcaggctg gtgaaggtcg ccgcggccgt ccggcgcgac
     1201 ggccgcgagc cgatgcgcat ctggctgatc tcgtccatcg gcgtggcgtg accccagtag
     1261 accagcagtt ccggcgtctc gtagatgagt tgccggtagg cgcggtagga cagcgccgcc
     1321 aactcgtcca ttgccgcccg ccactccggc tgcgggcgca cttgcaggcg gtagtgctcc
     1381 ggcgcgctgg cgatgaggac ggcatggacg acctgttcca gatggcgctc ggcgatggcc
     1441 gggtggctat agcggtcgga aatgacttcg ccctgctcgg tgatgcggat gcgcccgccg
     1501 acggagccgg gcggctgggc caggatggcg cggttggccg ggccgccgcc gcgggcgatg
     1561 gtgccgccgc ggccgtggaa gagggtcatg accacatcgt gcccgtggca gaccgcggcc
     1621 agcgcttcct gggcctgata caactcccag ttggcggcca ggtagccggc gtccttgttg
     1681 ctgtcggagt agccgatcat gatcgtctgg cggcggccga cacgctccag gtggggggca
     1741 tagccggggt ggtcgaacag cgcggccata atgtccgggg ccgcgcgcag gtcgtcgcgc
     1801 gtctcgaaca gcggcgcgaa agtcagcccc tccgcgccgt cctcgcccgg cgacaggcca
     1861 tgccagcggg ccagcagcag cggggcgagg atgtcttccg ggccgtgggt catgctgacg
     1921 atgtaggggc cgagcagttc cgggccgtaa tagtcaaccg cccgcttgat gacggcgaag
     1981 aggtccagtg tctcgcgcgc ctcggctgag aggtggtcga agctggacag gtcgggcgcg
     2041 ggccgggtca gggcgtcggt cagcacggcg gcgcggccgg ggccgtcgag cgcgccgaag
     2101 ccggcacacc agtcgaggcg tcccagcagc tcgtcgagga cgctggtgtt gtactcgctg
     2161 tactggcgca ggtcgagttg ggcggcgtgg aggccgaaga tgcgcgcctg cgacagggtg
     2221 cggtcgagtc cggcgcggcg gatgtcggcc atgccgccct cgcgcagcga gcgggccatc
     2281 agttccaacg gcgcgagcag gttctcctgc cggcgcatgc gcaggggcgg cgccggcttg
     2341 ccgcgcagcc ggccaaccat gtcgccggcc gacgcctcgg ccagatcggt gcccagttgg
     2401 gcagtgtgca ggccgtaggt ctcgttgggg tagcggtcgc gcaggtaggc caggtgggcg
     2461 tttaactcgc cataggcgcg caccgcctcg gccagcgcgg gcgacacggg ggccaggtcg
     2521 tcgctgatgc tcagcgagcg gtcgaggccg cgcgtggcgg cgcggtgctt ctccagcgcc
     2581 aggccgcg
//
LOCUS       DAOSMQ010000470         2583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_39924_length_2583_cov_4.832674, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000470 DAOSMQ010000000
VERSION     DAOSMQ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39924_length_2583_cov_4.832674"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..972
                     /locus_tag="PK829_12930"
     CDS             22..972
                     /locus_tag="PK829_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOU42163.1"
                     /translation="MPAWTLVWGEAAGPVCGPDEVLVDVSAAAVNRADLWQARGHYPP
                     PAGASDILGLEMAGVVREVGPAVQTFRPGDRVCALLPGGGYAELASVPAGMLLRLPDD
                     WTFAQGAALPEVWYTAFINLFDEGQLKAGETALIHAGASGVGTAAIQLAADARARVLA
                     TAGSDEKTARCRELGAELAINYKQADFLAEVLAATGGAGVDVILDPVGGGYLARNIAA
                     LRRFGRLVLIANLGGTRGELDTGQVLGKRLRIIGSTLRGRPVAEKIAITRRFEAEVWP
                     KLADGRLRPIIDRVWPIDQAQAAHDYVLADRNIGKVILEI"
     gene            complement(1068..2363)
                     /locus_tag="PK829_12935"
     CDS             complement(1068..2363)
                     /locus_tag="PK829_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HOU42164.1"
                     /translation="MDKRKLGFLIVLVALLAVLLASAPGPVVAETPAGHLDPGTQFFV
                     PRASAQARAHINWLYNSGRRADARLLRVMTNTPQAVWLTDPRPARVQEEVRRTVRQAN
                     GQGKVPVLVAYNIPFRDCAQFSAGGATTVSQYKTWIDNFAAGIGNLKAIVILEPDGLG
                     IIPWYKQFRGLPGEAGSYEWCQPADADEATAANDRFAMLNYAVDALKARPNTSVYLDG
                     THSAWLGAGDTAHRLVQAGVLRADGFFLNVSNYVQTERLQKYGAWVSKCIWFATDPAS
                     WGNGHFDWCASQYYPADPNDFSTWHLTDQWYIDNVESQTWAYPGEEGLSGFVIDTSRN
                     GQGPWTPAASYPDPQVWCNPPGRGVGLRPTADTGNPMLDAYLWIKIPGESDGECTRGL
                     GPAGQTVDPEWGVIDPAAGHWFRPMALQLARNANPPLLP"
BASE COUNT          412 a          799 c          932 g          440 t
ORIGIN      
        1 ctggccgccg ggaactgggg gatgcccgcc tggacgctgg tctggggcga ggcggccggg
       61 ccggtctgcg ggccggacga ggtgttggtg gacgtgagcg cggcggccgt caaccgggcc
      121 gacctgtggc aggcgcgcgg ccactacccg ccacccgccg gcgcgagcga tattctgggg
      181 ctagagatgg ccggcgtcgt ccgcgaggtc ggcccggccg tgcagacgtt ccgccccggc
      241 gaccgcgtct gcgccctgtt gcccggcggc ggctatgccg aactggccag cgtgcccgcg
      301 gggatgctgc tgcggctgcc cgacgattgg acgtttgccc agggcgcggc cctgccggag
      361 gtgtggtata cagccttcat caacctcttt gacgagggcc agctaaaggc gggcgagacg
      421 gcgctcatcc acgccggggc cagtggcgtg ggcacggcgg ccatccagct ggcggccgat
      481 gccagggcgc gtgtcttggc cacggccggc agcgacgaga agacggcccg ctgccgcgaa
      541 ctgggcgcgg agctggccat caactataag caggccgact tcctggccga ggtgctggcg
      601 gccaccggtg gcgcgggcgt cgatgtcatc ctcgaccccg ttggcggcgg ctatctggcg
      661 cgcaacatcg ccgcgctgcg ccgtttcggc cggctggtgc tcatcgccaa cctgggcggc
      721 acgcggggcg aactggacac cggccaggtg ctgggcaaac ggctgcgcat catcggctcg
      781 acgttgcgcg gccggcccgt ggccgagaag atcgccatca cccgccgctt cgaggccgag
      841 gtctggccga agctggccga tgggcggctg cggccgatca tcgaccgcgt ctggcccatt
      901 gaccaggccc aggccgcgca tgactatgtg ctggcggatc ggaatatcgg caaggtgatc
      961 cttgagattt aggcgcacgg gttgcctgct aaggcccagg gtcagacaaa aaagggagca
     1021 ggccgcttcg cctgctccct caggtatgaa agttactccg ccaacgccta aggcagcagc
     1081 ggcgggttgg cgttgcgcgc cagttgcagc gccatcggcc ggaaccagtg gccggcggcc
     1141 gggtcgatga cgccccattc gggatcgacg gtctggccag ccgggccgag gccgcgggtg
     1201 cattcgccgt cagactcgcc ggggattttg atccacagat aggcgtcgag catggggttg
     1261 ccggtgtcgg ctgtcgggcg cagccccacg ccacgcccgg gcgggttaca ccacacttgc
     1321 gggtcagggt agctcgcagc cggcgtccac gggccttgcc cgttgcggct cgtgtcgatg
     1381 acgaaaccgg atagcccttc ctcgccgggg taggcccagg tctggctctc cacattgtcg
     1441 atgtaccact ggtcggtcag atgccaggtg gaaaagtcgt tggggtcggc cgggtagtat
     1501 tggctggcgc accagtcgaa gtgcccgttg ccccaggagg ccgggtcggt ggcgaaccag
     1561 atgcacttgc tgacccacgc gccgtacttc tgcaggcgtt cggtctggac gtaattggag
     1621 acgttcagga agaagccatc ggcgcgcaag acgccggcct gcaccaggcg atgggccgtg
     1681 tcgcccgcgc ccagccaggc gctgtgggtg ccgtcgaggt agacagaggt atttggccgc
     1741 gccttcaggg catcgacggc gtagttgagc atggcgaagc ggtcgttggc ggcggtggcc
     1801 tcgtcggcgt cggccggttg gcaccattca tagctgcccg cctcgccggg caggccgcgg
     1861 aactgcttgt accaggggat gatacccagc ccatccggct ccaggatgac gatcgccttc
     1921 aggttgccga tgccggcggc gaagttgtcg atccaggtct tatactggct aacggtcgta
     1981 gcgccgccgg ccgagaactg ggcacagtcg cggaagggga tgttgtaggc cacgaggaca
     2041 gggactttgc cctggccgtt ggcctgccgg acggtgcgtc tgacctcctc ctgcacgcgt
     2101 gccgggcgag gatccgtcaa ccagaccgcc tggggcgtat tggtcatgac gcgaagtagc
     2161 ctggcgtcgg ctcggcggcc actgttgtag agccaattga tgtgcgctct ggcctgggcg
     2221 ctggccctgg ggacgaagaa ctgcgtgccg gggtcgagat ggccggccgg cgtttcggca
     2281 acgacagggc cgggcgcgga agccagaagc acggccaaca gagcgacgag aacgatgagg
     2341 aatccgagct ttcttttgtc catttcctgc tcctttgaat ggggttgaag tcagcgccgg
     2401 gcattttggc ggccgagggg ggaatgtctt ccaggctatc agaacattcc tcgcggacgg
     2461 gtaaaaaggc aggggtcagg caacgggcca caacaccctg tgctttacct gtttctaaat
     2521 catagtatca ctcaagttgc taccttgagc ttaacaagaa gaagtccatg aactattctc
     2581 agg
//
LOCUS       DAOSMQ010000471         2578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_40044_length_2578_cov_5.279033, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000471 DAOSMQ010000000
VERSION     DAOSMQ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40044_length_2578_cov_5.279033"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PK829_12940"
     CDS             complement(<1..1346)
                     /locus_tag="PK829_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42165.1"
                     /translation="MNRRLLFSLVLFLLLVLSVSGASATDEPLPRDLDALRARYDRPA
                     ATEMAGTPAAPEPADAPPATDTTPLAADVDHLVSWSELAFESARSGMWAIYRMKPNFT
                     EIVPAAVSSDFHNSSPALRPRGADRLLYVSLRNDNYDIYSSLPNGSDEKRLTDYRWDD
                     LMPAWSPDGTQIAFQSERTGGTDIYVMNADGSGLRRLTTAEQFDGHPTWSPDGAQIAF
                     SSARTGRYQIWVMNADGGGQRQLTDQFAALYPAWSPDGGTIAFSGLFSYRPEDPFLDI
                     LLMNGDGSAVRYFGGAFNRDAHSISWSPDGTYAAYVLTHWEIDHDAWQAQDSYLHLGS
                     YDGVSVHDKKIYTDDPWVLSVSWASRDSQPPGPCSVDTPAVRAWRSAYVTWNATDDLS
                     GVARYDVEWRRAGATEWRALALFAPAANFTLDERAAGEIIEWRCRATDAGFNRADWA"
     gene            complement(1427..>2578)
                     /locus_tag="PK829_12945"
     CDS             complement(1427..>2578)
                     /locus_tag="PK829_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOU42166.1"
                     /translation="VLDFTALLPGPFATMVLADLGAEVVRVESPTRPDMVRFLPPFDG
                     DISAWHGVLNRNKRSVALDLKRPEADAAVRRLVADGGYDIVIEQFRPGVMDRLGIGYE
                     ALRAVNPAVIYCALTGYGQTGPLRDRAGHDINYLALSGALSYSGRAGDGPPPLGFQVA
                     DIGGGAMGALVGLLAAVVHRAATGRGQFIDVSMFDLMVAWQAHLISHSLVAGETPMPE
                     SMALNGGRAYDLYATQDGRWLSVGSLEPKFWQGFCAAIGRPDLVAAGLSLDPAAVAAA
                     KTAVRHVIAARPLAEWVAVFAPLDVCVEPVLTVPEMLAGPQVAARGLVVDVPRPGGGT
                     QRQVANPWRFSDGAAEYRHAGAPLGADTNDVLTAAGFSDDEIARLLAAA"
BASE COUNT          398 a          860 c          955 g          365 t
ORIGIN      
        1 gcccaatcgg cgcggttgaa gccggcatcg gtagcccggc agcgccactc gatgatctcc
       61 cccgcggcgc gttcgtccag cgtgaagttg gctgccggcg cgaacagcgc cagcgcccgc
      121 cactcggtcg cgccggcgcg acgccactcc acgtcatagc gggcgacgcc ggagaggtcg
      181 tcggtggcgt tccaggtgac gtaggcgctg cgccaggcgc gcacggccgg cgtatcgacg
      241 ctgcacggcc cagggggctg gctgtcacgc gaggcccaac tgaccgacag gacccacggg
      301 tcgtccgtgt agatcttctt gtcgtgcaca ctcacgccgt cgtagctgcc caggtgcaga
      361 tagctgtcct gagcctgcca ggcgtcatgg tcgatttccc agtgggtcag gacgtaggcc
      421 gcgtatgtgc cgtcgggcga ccaggagatg gaatgggcgt ccctgttgaa cgcgccgcca
      481 aagtagcgaa cggctgagcc gtccccgttc atgagcagga tgtcgagaaa agggtcttcg
      541 ggtcgatagc taaagaggcc gctgaaggcg atggtgccgc cgtcgggcga ccaggcgggg
      601 tagagcgcgg caaactggtc tgtcagctgc cgttgcccgc cgccgtcggc gttcatgacc
      661 cagatctggt agcggcccgt gcgggccgag gagaaggcga tctgcgcgcc gtcgggcgac
      721 caggtgggat ggccgtcgaa ctgctcggcc gtggtcagcc gccgcaggcc gctgccatcg
      781 gcgttcatga cgtagatgtc agtgccgccc gtgcgctccg actggaaggc gatctgtgtg
      841 ccgtcgggcg accaggcggg catcaggtca tcccagcggt aatcggtcag gcgtttttcg
      901 tcgctgccgt tgggcaggga tgagtagatg tcgtagttgt cgttgcgcag cgagacgtag
      961 aggaggcggt cggcgccgcg cggccgcagg gccgggctgc tgttgtggaa gtccgacgag
     1021 acggccgccg gcacgatctc ggtgaagttg ggcttcatgc gatagatggc ccacatgccg
     1081 ctgcgcgcgg actcgaacgc cagctcgctc caactgacca ggtgatcgac gtccgcggcc
     1141 agcggggtgg tgtctgttgc cggtggcgca tcggccggct cgggcgcggc tggcgtgccg
     1201 gccatctcgg tggcagccgg acggtcatag cgcgcgcgca gggcgtcgag gtcgcgcggc
     1261 aacggctcgt ccgtcgccga cgcccccgac acagacagaa ccaacagcag gaacaggaca
     1321 agcgagaaga gaaggcggcg attcatgttc caggctccct cagaagattg tgggtcgggt
     1381 taaggagcgc cgcggacgag cgcggcgcgt cgagacgtgc ggtgacctag gccgccgcga
     1441 gcagccgggc gatctcgtcg tcgctaaacc cggccgcggt caatacatcg tttgtgtccg
     1501 ctcccagcgg cgcgccggcg tggcgatact ccgccgcgcc gtcgctgaag cgccacgggt
     1561 tggccacctg ccgttgcgtg ccgccgcccg gccggggcac gtccaccacc aggccgcgcg
     1621 cggccacctg cggcccggcc agcatctccg gcacagtcag caccggctcg acgcagacat
     1681 ccagcggggc gaagacggcg acccactcgg ccagcggacg ggcggcgatg acgtggcgca
     1741 cggccgtctt ggccgcggcg acggcggccg ggtcgaggct aagccccgcg gccaccaggt
     1801 cgggccggcc gatggccgcg cagaagccct gccagaactt cggctccagg ctgccgacgg
     1861 acagccagcg gccgtcttgc gtggcgtaga ggtcataggc ccggccgccg ttgagggcca
     1921 tgctttccgg catgggcgtc tcgccggcca ccaggctgtg gctgatcaga tgcgcctgcc
     1981 aggcgaccat caggtcgaac atgctgacat cgatgaactg gccgcggccg gtggcggcgc
     2041 ggtggacgac ggcagccagc agccccacca gcgcccccat cgccccgccg ccgatgtcgg
     2101 ccacctggaa gcccagcggc ggcgggccat cgccggcacg gccgctgtag ctgagcgcgc
     2161 ccgacagggc caggtagttg atgtcgtggc cggcgcggtc gcgcaggggg ccggtctggc
     2221 catagccggt cagggcgcag tagatgacgg ccgggttgac cgcccgcagc gcctcgtagc
     2281 cgatgcccag ccggtccatg acgcccggcc ggaactgctc gatgacgata tcgtagccgc
     2341 cgtcggccac cagccggcgc acggcggcgt cggcctccgg ccgcttcagg tcgagggcca
     2401 cggagcgctt gttgcggttg aggacgccat gccaggcgga gatgtcacca tcgaagggcg
     2461 gcaggaagcg caccatgtcc ggccgggtgg gcgactcaac gcgcacgacc tccgcgccca
     2521 ggtcggccag caccatcgtg gcaaaggggc cgggcaggag cgcggtgaag tcgaggac
//
LOCUS       DAOSMQ010000472         2561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_40387_length_2561_cov_4.755387, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000472 DAOSMQ010000000
VERSION     DAOSMQ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40387_length_2561_cov_4.755387"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..687
                     /locus_tag="PK829_12950"
     CDS             199..687
                     /locus_tag="PK829_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOU42167.1"
                     /translation="MDTTQQMQMTIDRDALTITVNRAFDAPRERVWRALTEPALIARW
                     WGGGAGSQTTHVDQFDLRPGGAWRFVERGENGEDAFRGEFREVTPPQRIVQTFEYEPW
                     AGHILVETMTLSEQAGGTLLTVIEQFSNLEDLDGMVQSGMEDGAAKSYRQLAELLATL
                     GD"
     gene            complement(823..>2561)
                     /locus_tag="PK829_12955"
     CDS             complement(823..>2561)
                     /locus_tag="PK829_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU42168.1"
                     /translation="DDNLHNFSSRGPREDGGFKPEVVASGAAISSIPMWQAQGCLAQV
                     CPVGYALFNGTSMAAPQATGGVAQLISAAQQAKVQHLPEQIRQAVRSSARYLTGRYQA
                     YEQGNGLMNVPDAWDVLSAKVTTVDITSSVPVHTALSAFLATPGYGVGINDREGVMAG
                     DSYSRTYTFRRTSGANGNKLYNVNWVDNDGTFSSAGTIVLPKNANVSFPVNINPTTSG
                     AHSAILNLDDPGNPGIEYQTMNVVVAADQFNAGNSYTVTKSGSLGPGQFVTYFFNVPA
                     GTPAFKVDMTGGGATVGAGAIRFMRWHPYGVGIDNNGVSNCYNPSIGGCTTGSALSRT
                     TTNPFPGVWEVTVDARRNSDAVSAPFTLTASILGATVAPNPDVIASATVGVPVARSYT
                     ITNIYGAFTGKAIGTALGSAYIDTPTIANGATQSFNVTVPAGSTALRATIGSTSDPAA
                     DLDLFVYNCVSGPCVLVGSQADGDSEESVTVASPAAGAWRVDVQAYNVPNGTTTYKYI
                     DVISNPAFGAITVTDADALRAAGASWMVPASVTANAVPATGRVLYGNVQVRTNTNVLV
                     GQGDVIVQSVTP"
BASE COUNT          437 a          807 c          901 g          416 t
ORIGIN      
        1 acctgggcgg ccggacgcgg atgcgcgtct acaccgacca gaagcccgac ggcgagatgg
       61 gtgacatggc tgtactgggt tggaaccagt cgttcgataa gttggccgcc agcctgcgcg
      121 gctaggcggg gacgcgatag ccggcccagg ccggccctac tggcaatgga tcacgacgac
      181 taccgaagga gaatagaaat ggacacgacc cagcagatgc aaatgaccat cgaccgcgac
      241 gccctgacca tcaccgtcaa ccgcgccttc gacgccccgc gcgagcgcgt ctggcgggcg
      301 ctgacagagc cggcgctgat cgcccgctgg tggggcggcg gcgcgggcag ccagacgacc
      361 cacgtcgacc aattcgacct gcgccccggc ggcgcgtggc gctttgtgga gcgcggcgag
      421 aatggcgaag acgctttccg cggcgagttc cgcgaggtca cgccaccgca acgcatcgtg
      481 cagacgttcg agtatgagcc gtgggccggc cacatcctgg tcgagacgat gacgctgagc
      541 gagcaggccg gcggcacgct gttgaccgtc atcgagcagt tcagcaacct cgaagaccta
      601 gacggcatgg tgcagtcggg catggaagac ggcgcggcga agagctaccg gcagttggcc
      661 gagctgttgg ccacgctggg cgactagctg ccgtcacctc agcggtacta tcagagcaaa
      721 aggcccgcgg gattgttcgt cccgcgggcc tttgtgatta gaagcctgcc ctaccgccag
      781 ccgggccgcg gagcggccgg ccgcgagagg gcctaaacgc ccttacggcg tgacgctctg
      841 gacgatgacg tcgccctggc ccaccaggac gttggtgttg gtgcgaacct ggacgttgcc
      901 gtacagaacg cgcccggtcg ccgggacggc gttggccgtc accgacgcgg gcaccatcca
      961 cgaggcaccg gctgcgcgca gcgcgtcggc gtcggtcacg gtgatcgcgc cgaacgccgg
     1021 gttgctgatc acgtcgatgt acttgtacgt ggtcgttcca ttcggaacgt tgtaggcctg
     1081 gacatcaacc cgccacgcgc ccgcggccgg gctggcgacg gtcaccgatt cctcggagtc
     1141 gccgtcggcc tgggagccga ccagcacaca aggcccactg acgcaattgt agacgaacag
     1201 gtccaggtca gcggccggat cggacgtgct gccgatcgtc gcccgcagcg ccgtcgagcc
     1261 ggcgggaacg gtcacgttga aggactgcgt ggctccgttg gcgatggtgg gcgtgtcgat
     1321 gtaggcgcta cccagcgctg taccgatagc cttgccagtg aacgcgccat agatgttggt
     1381 gatggtgtag ctacgggcca ccggcacgcc aaccgtcgcc gaggcgatga catccgggtt
     1441 cggcgcaacc gtcgccccca ggatggacgc ggtcagcgtg aacggcgcgc tgacggcgtc
     1501 ggagttgcgc cgcgcgtcaa ccgtcacttc ccacacgccc gggaacgggt tcgtggtcgt
     1561 gcggctgagg gcactaccgg tggtgcaacc accaatgctg gggttgtagc agttgctgac
     1621 gccgttgttg tcgatgccga cgccataggg gtgccaacgc atgaagcgga tcgcgcctgc
     1681 gcccacggtc gcgccgccgc cggtcatgtc caccttgaag gcaggcgtgc cggccggaac
     1741 gttgaagaag taggtcacga actggcccgg cccaagcgaa ccgctctttg tgaccgtgta
     1801 gctgttgccg gcgttgaact ggtcggcagc cacgacgaca ttcatcgtct ggtactcgat
     1861 gccggggtta cccggatcgt ccagattgag gatagccgag tgggcgccgc tggtcgtcgg
     1921 gttgatgttg acggggaacg agacgttggc gttcttgggc agaacgatcg tgccggccga
     1981 actgaacgtg ccgtcgttat cgacccaatt cacgttgtag agcttgttgc cgttcgcgcc
     2041 actggtgcgg cggaaggtgt aggtgcggct atagctgtcg ccagccatga cgccctcgcg
     2101 gtcgttgatg cccacgccgt agcccggcgt agccaggaag gcgctcagcg cggtgtggac
     2161 gggcaccgac gaggtgatgt cgacggtggt caccttggcg ctcagaacgt cccacgcgtc
     2221 gggaacgttc atcaagccgt tgccctgctc ataggcctgg tagcggccgg tcaggtagcg
     2281 cgcgctggag cgcaccgcct ggcggatctg ctccggcagg tgctgcacct tggcctgctg
     2341 ggcggcgctg atcagctggg caacgccacc ggtggcctgc ggagcagcca tcgacgtgcc
     2401 gttgaacagc gcgtagccga ccgggcagac ctgggccaga cagccctgcg cctgccacat
     2461 ggggatgctg gagatggccg cgccggaagc gacgacctcg ggcttgaagc cgccatcctc
     2521 gcgcgggccg cgcgacgaga agttgtgaag attgtcatct t
//
LOCUS       DAOSMQ010000473         2560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_40407_length_2560_cov_4.208782, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000473 DAOSMQ010000000
VERSION     DAOSMQ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40407_length_2560_cov_4.208782"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PK829_12960"
     CDS             complement(<1..1364)
                     /locus_tag="PK829_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU42169.1"
                     /translation="MANYDFLRKIPLFSSLSDEDLSSLCQIVREFHLPAGKVLFEEGS
                     VGDMAYIIERGEVEIIKLSQKREILLAKRGPGEVFGEIALLFDTPRTAAVRARTDTDL
                     IGIGREDLRNLLQISPSAAASMFDIVLSRWQNTQNLLRQSEKMAQLGTFTAGIAHELN
                     NPAAAVQRSTAQLETVLFDSAHTFMLLVRQGLSGEQQATLEQFVRRAQEQARSMPPMD
                     PLARSDREAELEEWLEDRGVQDPWDCAATLVNLEFTDDDLKRLASGYGQAQLPVIINW
                     LNNNYTIFNLLAELQQGAQRISAIVKALKSYTYLDQADIVRYNVAEGLENTLLILRHK
                     LKAGIKIVREYDPALPQIDAHGGELNQVWTNLIDNAVDALRNGPDSAPQPNATLTLRT
                     RQEGKWVIVTVEDNGPGIPPDVLPRIFDAFYTTKPVGQGTGLGLDISYNIVVRKHFGD
                     IRVKS"
     gene            complement(1389..>2560)
                     /locus_tag="PK829_12965"
     CDS             complement(1389..>2560)
                     /locus_tag="PK829_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42170.1"
                     /translation="ERILALAEQAGRPDAEAYGRWAMLLFHVNFDADFARAVAEGERA
                     LALARQHGLAELEAYVLNDMGRTYTAAHRMTDALAALAAAFESWRVLGNEPMQADAAS
                     IWSQGLLLRGDLAEAERLAREGLAIGRRINNVWAQSFNALSLGQVLFRQGRAGEALRL
                     TRWAMEISAQGGFSGPGVNAPLILHWVSGLLGGPAVAHEQLRETMTGTGLADHPFMGP
                     LAKGMERYFAGDAAAGFALSAKLEWPGVARLGPIGVMLYTTPIAMGVAAGRADEALAM
                     ADALLDRLAREGFAAFRPDALALRGAALRALGRADEARAAYEQALDEANRLGVRWDEW
                     NTLAALHALEPDAARAADYRRRAAAAIDFLAGQLDDELRAAFLGRADVQRLRGDR"
BASE COUNT          391 a          843 c          890 g          436 t
ORIGIN      
        1 gacttcacac ggatgtcgcc aaagtgcttg cggacgacga tgttgtagct gatatccagc
       61 cccagccccg tgccctggcc aaccggcttg gtggtataga aagcgtcgaa gatgcgcggc
      121 agcacgtcgg gtggaatgcc cggcccgttg tcctcgacgg tgacgatgac ccatttgccc
      181 tcttgccgcg tgcgcagagt gagggtggcg ttgggttgcg gcgcgctgtc ggggccgttg
      241 cgcagtgcgt caacggcgtt gtcgatcagg ttcgtccaga cctggttcag ttcgccgccg
      301 tgggcgtcga tctgcggcag agccgggtca tattcgcgca cgatcttaat gcccgccttc
      361 agcttgtgac gcaggatcag cagtgtattc tccaggcctt cggccacgtt gtagcgcacg
      421 atgtcggcct ggtctagata agtgtaggac ttcagcgcct taacgatggc ggagatgcgc
      481 tgcgcgccct gttgcaactc ggccagcaga ttgaagatag tgtagttatt gttgagccag
      541 ttgatgatga ccggcagctg ggcctgacca tagccagacg ccaggcgctt caggtcgtcg
      601 tcggtgaatt ccagattgac caacgtcgcc gcgcaatccc aggggtcctg gacaccgcgg
      661 tcttccagcc attcctccaa ctcggcctcg cggtcgctgc gggccagagg gtccatcggc
      721 ggcatcgacc gggcctgctc ctgggcgcgg cggacgaact gctccagcgt ggcttgctgt
      781 tcgccggata gcccctggcg cacgaggagc atgaaggtat gggcggagtc gaagagcacc
      841 gtctccaatt gcgctgtgct gcgctggacg gccgcggcgg ggttattcaa ttcatgggcg
      901 atgccggccg tgaaggtgcc caactgggcc atcttctcgc tctggcgcag caggttctgg
      961 gtgttctgcc agcgcgacag aacgatgtcg aacatcgacg ccgccgccga cgggctgatc
     1021 tgcaacaggt tgcgcaggtc ttcgcggccg atgccgatca ggtctgtgtc cgtccgcgcc
     1081 cgcacggcgg ccgtgcgcgg tgtatcgaat agcaaggcaa tttcgccgaa gacctcgccc
     1141 ggcccgcgct tggccagcag aatctcgcgc ttctggctga gcttgatgat ctccacctcg
     1201 cccctctcga tgatgtaggc catgtcgccg acgctgccct cctcaaagag cactttgccg
     1261 gcgggcaagt ggaattcgcg cacgatctgg cacaggctgg acagatcttc gtcggagagc
     1321 gacgagaaca ggggaatctt gcgcagaaag tcgtagttag ccataggtga tttcgtcgtc
     1381 gcggcggtct atcggtcgcc tctcaggcgt tggacatcgg cgcggccgag aaaggcggcg
     1441 cgcaattcgt cgtcgagctg cccggccagg aagtcgatgg ccgcggccgc gcggcggcgg
     1501 tagtcggccg cgcgggccgc gtctggctcc agcgcgtgca gcgcggccag cgtgttccac
     1561 tcgtcccagc gcacgcctag ccggttggcc tcgtcgaggg cctgctcata ggcggcgcgg
     1621 gcctcgtcgg cacggcccag cgcgcgcagc gccgcgccgc gcagggccag ggcgtcgggc
     1681 cggaaggcgg cgaaaccctc gcgcgccagc ctatccagga gggcatcggc cattgccagc
     1741 gcctcgtccg cgcgccccgc ggccaccccc atcgcgatgg gcgtcgtgta gagcatgacc
     1801 ccgattgggc caaggcgggc cacgcccggc cactccagtt tggcgctgag cgcaaagccc
     1861 gcggcggcgt cgccggcgaa gtagcgctcc atgcctttcg ccagcgggcc catgaagggg
     1921 tggtcagcca ggcccgtccc ggtcatcgtc tcgcgcagtt gctcgtgggc cacggccggc
     1981 ccgccgagca ggccggagac ccagtgcagg atcagcggcg cgttgacgcc cggcccgctg
     2041 aaaccgccct gggcgctgat ctccatggcc cagcgggtga ggcgcagggc ctcgccggcg
     2101 cgcccctggc ggaacagcac ctggccgagg gagagggcgt tgaagctctg cgcccagacg
     2161 ttgttgatgc gccggccgat ggccaggccc tcccgcgcca ggcgctcggc ctcggccagg
     2221 tcgccgcgca gcagcagccc ttgcgaccag atgctggccg cgtcggcctg catcggctcg
     2281 ttgcccagca cgcgccagct ctcaaaggcg gcggccaatg cggccagggc gtcggtcatg
     2341 cgatgggcgg cggtataggt gcggcccatg tcgttgagca cataggcctc cagctcagcc
     2401 aggccgtgtt gccgcgccag ggccagggcg cgctcgcctt cggccacagc gcgggcgaag
     2461 tcggcatcga aattgacgtg gaacaatagc atcgcccagc ggccataggc ctcggcgtcg
     2521 ggccggccgg cctgctcggc cagggccagg atgcgttcgg
//
LOCUS       DAOSMQ010000474         2554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_40547_length_2554_cov_4.747099, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000474 DAOSMQ010000000
VERSION     DAOSMQ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40547_length_2554_cov_4.747099"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PK829_12970"
     CDS             <1..827
                     /locus_tag="PK829_12970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOU42171.1"
                     /translation="AETVDVGHRAEFYDKVGVVRDMMQNHVMQLLALVATEPPTSFDA
                     EAIRNEKAKVFQSIRPIRPENVASCTVRGQYRTYRDTKGVAPGSTTPTYAAIRYYVDN
                     WRWQGVPFYMRSGKALAAKSTEITIFFKRPPHVMFPMPEGAQLAPNYLSICIQPDEGI
                     EFSFQAKVPDTAAEMRTVDMSFQYEQSFGAMAIPEAYERLLLDVIKGDPSLFTRSDAI
                     ELCWQLIDPILEAWQSKHAPPLSFYESGTWGPVEADALIAHDGYHWTHGCGQEGEA"
     gene            complement(824..>2554)
                     /locus_tag="PK829_12975"
     CDS             complement(824..>2554)
                     /locus_tag="PK829_12975"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOU42172.1"
                     /translation="TPNPARSPLMHMMAGIYGRYQTALARQGGLDYDDLIWLAADRLE
                     HEPDLTTHLRARWPVILEDEAQDSVPLQETLLEQLTGPEGNWVRVGDPNQAVTSTFTA
                     AHPRFFSRFIDRPDVTARTLPNSGRSAPLIIGAANAILNWTIDNHPVPEVRANAFRRQ
                     DILPAPPGDAQPNPPDSEAEARIRVYRHREEEELPTVARLASEYAQEHPEQTLAILVP
                     THQAGYAIARALDALNADYDNLLRGSGREREVAAALHAVLAVLANPLDTRALQSAHSG
                     LSELRESGPYYDPYPDDSEQIERFRALLRSVHRPETLLFPREDEELVEALPAGVATEE
                     DVARLERLADFLRANFDLRTLPVDDLTLALADALFANDSASATTEDAATHELDLAIAY
                     QIAAQLRIWREAQPEWRLPELVAQLQIVASGRRSLNIMRGAELGYEPAPGRISLATQH
                     SSKGLEWDAVFLVGIDGMWIPGSLDAPFLGVHDFLGGDANAEATAQLRYLMGAETGLH
                     PNRTATESAHIEVICERLRLLYVGITRARRFLQISRSRTTRRGRSEQESEPATVLGVL
                     YRYLQERSES"
BASE COUNT          416 a          822 c          886 g          430 t
ORIGIN      
        1 ccgccgagac ggttgatgtc ggccaccggg cggagttcta cgacaaggtc ggcgtcgtgc
       61 gcgacatgat gcagaaccac gtcatgcagc tactggcgtt ggtggccacc gaaccgccga
      121 cttccttcga cgccgaggcc atccgcaacg agaaggccaa ggtgttccag tcaatccggc
      181 ccatacggcc ggagaacgtc gccagttgca cggtgcgcgg ccagtaccgc acctatcgcg
      241 atacgaaggg ggttgcgccc ggctcgacga cgcctactta cgccgccatt cgctactacg
      301 tcgacaactg gcgctggcag ggcgtgccct tctacatgcg ttcgggcaag gcgctggcgg
      361 ccaagtcgac ggagatcacc atcttcttta agcggccgcc gcacgtgatg tttcccatgc
      421 ccgagggggc gcaactggcc cccaactacc tgtctatctg catccagccc gacgaaggca
      481 tcgagttttc ctttcaggcc aaagtgccgg acactgcggc cgagatgcgc acggtcgata
      541 tgtccttcca gtatgagcaa tcgttcggcg cgatggctat ccccgaggcc tacgagcggc
      601 tgctgctgga cgtgatcaag ggcgacccct cgctttttac gcgtagcgac gccatcgagc
      661 tatgctggca gctcattgac cccattctgg aggcctggca gtcgaagcac gcgccgccac
      721 tcagcttcta cgaatcgggc acgtgggggc cggtggaggc cgacgcgttg atcgcccatg
      781 atggctatca ctggacgcat ggctgtgggc aagaggggga ggcctaactc tcgctgcgct
      841 cctgcaaata gcgatacagc acccccaaca ccgttgccgg ctccgattcc tgctcgctgc
      901 ggccgcggcg ggtggtgcgg ctgcggctga tctgcaagaa gcggcgcgcg cgagtaatgc
      961 caacgtatag cagccgcaag cgctcgcaga tgacctcgat gtgggctgac tcggtggccg
     1021 tgcggttagg gtgcaggccc gtctccgcgc ccatcagata gcgcaattgc gcggtggcct
     1081 cggcattggc gtcgccgccc agaaagtcgt ggacgcccaa aaaaggcgcg tccaggctgc
     1141 caggaatcca catgccgtcg atgcccacca ggaagacggc gtcccactcc agccccttgc
     1201 tactgtgctg cgtggccaat gagatacggc cgggcgctgg ctcatagccc aactccgcgc
     1261 cgcgcatgat gttgagcgag cggcggccag aggcgacaat ctggagttgg gccaccagct
     1321 ccggcagccg ccactccggc tgcgcctcgc gccagatgcg cagctgggcg gcgatctggt
     1381 aggcaatggc cagatcaagc tcgtgggtgg ccgcgtcctc ggtcgtcgcg ctggcgctgt
     1441 cgttggcgaa aagcgcgtcg gccagagcca gcgtcagatc atcgaccggc agcgtgcgca
     1501 gatcgaaatt ggcccgcagg aagtcggcca gccgctccag gcgggcgaca tcctcctctg
     1561 tggccacgcc agccggcaac gcctcgacaa gctcctcgtc ctcgcgtggg aagagaagtg
     1621 tctccggccg gtgaacgctg cgcagcaatg cccgaaagcg ctcgatctgc tcgctgtcgt
     1681 cggggtaggg gtcgtaatac ggccccgatt cgcgcaactc cgacaggcca ctgtgggcgg
     1741 attggagcgc gcgcgtatcc agcgggttgg ccagcacggc caggacggcg tggagggcgg
     1801 cggcgacctc gcgctcccgg ccgctgccac gcaacaggtt atcgtagtcg gcgttcagcg
     1861 catccagagc gcgggctatg gcgtaaccgg cctggtgggt gggcacgagg atggccagcg
     1921 tctgctccgg gtgctcctgg gcatactccg acgccagccg ggccacggtg ggcagttcct
     1981 cctcctcgcg gtggcggtaa acgcggatgc gcgcctcggc ttcactgtcg ggcgggttgg
     2041 gctgggcgtc gcccgggggc gcgggcagaa tgtcctgccg ccggaaggcg ttggcgcgca
     2101 cttcgggcac ggggtggttg tcgatggtcc aattgaggat ggcgttggcc gcgccaatga
     2161 tgagcggggc ggagcggccg ctgttgggca gcgtccgcgc ggtcacatcc ggccggtcga
     2221 tgaagcgcga gaagaagcgc ggatgggcgg cggtaaaggt gctggtgacg gcctggttgg
     2281 ggtcgccgac gcgcacccag ttgccttctg gcccggtcag ttgctccagc agcgtctctt
     2341 gcagcggcac gctgtcctgc gcctcgtcct ccaggataac aggccagcgg gcgcgcagat
     2401 gggtggtcag gtcgggttcg tgctccagcc ggtcggccgc cagccagatg aggtcatcgt
     2461 agtccagccc cccctgccgg gccagcgccg tctggtagcg gccatagatg ccggccatca
     2521 tgtgcatcag cggcgagcgg gcggggttgg gcgt
//
LOCUS       DAOSMQ010000475         2554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_40549_length_2554_cov_4.390156, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000475 DAOSMQ010000000
VERSION     DAOSMQ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40549_length_2554_cov_4.390156"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="PK829_12980"
     CDS             complement(<1..1723)
                     /locus_tag="PK829_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU42173.1"
                     /translation="MDTPAEDKSKGLTPVRAADLRQQIEGLQAMVTDQQRALMRVPDA
                     NARATALRATAALVRQLVGLGESTRQLEQDLHAEAGEQNRLRALQEVGAAVNSSLDLE
                     VVLQQVMDAIVRLTRAERAMLLLDNGGALEVKMARNLSQETLAPGSLDISRSIIRRVA
                     ETGEAVVTINAQEDERFSSQHSIVSYKLRSILCAPLKLKGETIGVLYADNRVASGIFG
                     DADRDLLAAFADQAAVAIDNARLFRTVTEIKQLLDNVFASIASGVITIDAADRIALFN
                     RAAEIILGVPASAILSRGYRGVLPLLGLPIDHLVETVKADGRSRTAEVDLAAARRRAG
                     ATTVQLVVSPLRSPANEQLDGVALLLDDVSEKKRVESLRRYLPPALVDRVRDLDAAQR
                     PQRRTLTVLFADIRDFSRYGEYLDPEALIDLANSFFSEAVAVISEHQGLIDKFTGDAV
                     MALFNTPLNPQDDHTSQAVRAALRIQARLAARQREMPSDKVLHVGIGVHTGEAVVGNV
                     GSEQRKDYSAVGDVVNLCKRLQEMAGPDETLVSRVVYEQVRAYVQAEPLLPVQVKGRQ
                     TLEEVFRL"
     gene            complement(1846..>2554)
                     /locus_tag="PK829_12985"
     CDS             complement(1846..>2554)
                     /locus_tag="PK829_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU42174.1"
                     /translation="VPARVVSGYALGEYTPEAKNYRVRAVNAHTWVEVYFPGYGWIYF
                     EPTQSIPVVERPESGQVASGLTPPVPVPDANRALTDDELSDLERANGLLDGTDNQQNQ
                     GLLGGVPWWQVATGATLLIAAAAALWMANRYNQKVEGDVGRSFLRLGEWARVLGLAWR
                     PTQTPYEQADTLVAVVPQGQQPIRNLTRQYVLQLFSPTRSMDADFDPRQEWRQLRPML
                     IRRGIVNTLNRTRGQKK"
BASE COUNT          423 a          847 c          848 g          436 t
ORIGIN      
        1 ccagccggaa gacctcctcc agcgtctgcc gccccttcac ctgcaccggc agcaacggct
       61 ctgcttgcac gtaggcgcgc acctgttcgt agaccacgcg gctgaccagg gtctcgtctg
      121 gcccggccat ctcctgtaga cgcttgcaca aattcaccac atcgcccacg gccgaatagt
      181 ccttgcgctg ctcgctaccg acgttgccga cgacggcctc gccggtgtgg acgccaatgc
      241 cgacgtgcaa caccttgtcg gagggcatct cgcgttgacg ggccgccagc cgcgcctgga
      301 tgcgcagcgc cgcgcgaacg gcctgcgagg tgtggtcgtc ctggggattg agcggcgtgt
      361 tgaacagagc catcaccgcg tcgccggtga acttgtcgat taacccctgg tgctcgctga
      421 tgacggccac cgcctcagag aagaagctgt tggccagatc gatcagcgcc tcggggtcga
      481 ggtattcgcc atagcggctg aagtcgcgaa tgtcggcgaa gagcacggtc aacgtgcgac
      541 gttgcggccg ttgggccgcg tccaggtcgc gcacccggtc aaccagcgcc ggcggcagat
      601 agcgccgcaa gctctccacc cgcttcttct cggagacatc gtccagtagc agggccacgc
      661 catccagctg ctcgttggcc ggcgagcgca gcggcgacac gaccagctgc acggtcgttg
      721 ccccggcgcg gcggcgggcg gcggccaggt ctacttcggc cgtccggctg cggccgtcgg
      781 ctttcaccgt ctccaccagg tgatcgatgg gcaagcccag cagcggcaag acgccgcgat
      841 agccccgcga caggatggcg ctggcgggca cgcccaaaat gatctcagcg gcgcggttaa
      901 acagggcgat ccggtcggca gcatcaatgg tgatcacccc gctggcgatg gaggcgaaga
      961 cgttgtccaa caactgcttg atctcggtaa cggtgcggaa caggcgcgcg ttgtcgatgg
     1021 ctacagcggc ctggtcggcg aaggcagcca acaaatcgcg gtcggcgtcg ccgaagatgc
     1081 cgctggcaac gcggttgtcg gcatagagca cgccgatggt ctcacccttc agcttgagtg
     1141 gcgcgcagag gatggaacga agcttgtagc tgacaatact gtgctggctg gaaaagcgct
     1201 cgtcctcctg ggcgttgatg gtcaccaccg cctcgcccgt ctcggccacc cggcggatga
     1261 tgctacggct gatgtccagc gagccagggg caagcgtctc ctgagagaga ttgcgagcca
     1321 tcttcacttc tagcgcgccg ccgttgtcga ggagcagcat ggcccgttcg gcccgcgtca
     1381 ggcggacaat ggcgtccatg acctgctgga ggacaacttc cagatcgagc gaagagttga
     1441 cggccgcgcc aacctcttgc agggcgcgca ggcggttctg ttcgccggcc tctgcgtgga
     1501 ggtcctgctc cagttgtcgc gtcgattcgc ccagacccac cagttgccgc accagcgcgg
     1561 ccgtggcccg cagcgccgta gcccgcgcat tcgcgtcggg cacgcgcatc agggcgcgct
     1621 gctggtcggt gaccatcgcc tgtagccctt cgatctgctg ccgcaggtca gcggcgcgaa
     1681 caggcgtcag gcccttgctc ttgtcttcag ccggagtgtc caagatgtct tcgtgcgtcg
     1741 cgaggtcgtg tgttgtgtgg caaggaaaaa gccctgccgg cggcggatgg gccgcgcaga
     1801 cagggccatt atagcagaga acccacgcga gcgtgtcccg cgcgcctact ttttctggcc
     1861 gcgggtgcga ttcagggtgt tgacaatgcc gcggcggatg agcatcggcc gcagttgtcg
     1921 ccactcctgc cgcgggtcga aatccgcgtc catcgagcgg gtagggctga acagctgcaa
     1981 gacgtattgc cgggtcaggt tacgaatggg ctgctggcct tgcggcacga cggccacaag
     2041 cgtgtcggcc tgctcataag gcgtctgcgt cggccgccag gccaaaccca ggacgcgcgc
     2101 ccactcgccc agccgcaaga agctgcgccc cacatcaccc tccactttct ggttataccg
     2161 gttagccatc cagagggccg cagcggcggc gatcagcagg gtcgcgccgg tggccacctg
     2221 ccaccagggc acgccaccga gcagcccctg gttctgctgg ttgtctgtac cgtccaacag
     2281 gccgttggct cgttccaggt cgctgagttc gtcgtcggtc agggcgcggt tggcgtcggg
     2341 cacaggcacg ggcggcgtca gcccactggc cacctggccg gattcgggcc gttcgaccac
     2401 gggaatcgac tgggtcggct cgaaataaat ccagccgtag ccggggaagt agacctcaac
     2461 ccaggtgtgg gcgttaacgg cgcgcacgcg gtagttcttc gcctccggcg tgtactcgcc
     2521 cagggcatag ccgctgacca cgcgcgccgg cacg
//
LOCUS       DAOSMQ010000476         2549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_40665_length_2549_cov_3.174820, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000476 DAOSMQ010000000
VERSION     DAOSMQ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40665_length_2549_cov_3.174820"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /locus_tag="PK829_12990"
     CDS             <1..1903
                     /locus_tag="PK829_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42175.1"
                     /translation="RVGPPASTHLAAAVDPLVILCEDLHWADSASLDLIDDLYAALRD
                     AAPAAVLIVAAGRPSLLERRPGWANDAAGVGLLRLTALPPAQTQALVEEALQRVPRLP
                     DRLRHRIADVSGGNPFYVEELVKMYIEQGVVARGATDDEPWSVALDRLDAVRVPPTLV
                     GLIQARLDALPAAERRALQHAAVIGRVFWDSALAALAGAATPPPLTTALGREVVFRRT
                     PSTFADCQEFLFRHALVRDVAYETVLLSERRALHAHAARWLETHAAERLDECLSVLAA
                     HWDAANDATRAAAYLVRSAELALAAGNHGAACATLRRALELTAPSAATLLLLGEAAFM
                     ISDLPTASDALEQALNLAQTTGDRAAEANSLYWYSRVATARGDFDTARVALETALPIA
                     RAVGGATLARALEGLAGATGSRGDLPAATALGKEALALAREVGDAVLEMRCLALLGTF
                     ARLGDDLDSAEARFQAARALAHQAGNLEREATALLNLGDLAYRRERYDDARVAAHAAR
                     DLFRDQGLADAVAIAAGNLAQAELMLGERDAAVADARQALAAARAAGATPLALFALSV
                     FAQALAPTDRPRAAALLHLVIHHPATEYQDRREAAELLMHLGTAIVPATPLDLDTTIT
                     AILNNAASD"
     gene            complement(1900..2433)
                     /locus_tag="PK829_12995"
     CDS             complement(1900..2433)
                     /locus_tag="PK829_12995"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HOU42176.1"
                     /translation="MNDPIDTPPDEGERAAGEEHRRRMRFEFRDLIEDLIEDGRRRGL
                     FDDLAGQGRPLDLEANTYEGSAALTNKLMKDNDLRPPWLTRRVEVVGKIDALRADISR
                     TWGRYRAAFTAPGESHRSALTIGWDDSCQKWEATIASLNKEIESYNLKRPMSQLELFK
                     LRLADELRRVDAPRYLL"
BASE COUNT          319 a          965 c          884 g          381 t
ORIGIN      
        1 gcgggtgggg ccgccggcct ccacccacct ggccgccgcc gtcgacccgc tggtcatcct
       61 gtgcgaagac ctccactggg ccgatagcgc ctcactcgac cttatcgacg acctttatgc
      121 cgccctgcgc gacgccgccc ccgcggccgt gctgatcgtc gccgcgggcc ggccgagcct
      181 gctggagcgc cgccccggct gggccaacga cgcggcgggc gtcgggctgc tgcgtctgac
      241 tgccctgccg ccggcccaga cgcaggccct tgtggaggag gctctccagc gcgtgccccg
      301 gttgcccgac cggctgcgcc atcgcatcgc cgatgtgtcc ggcggcaacc cgttctatgt
      361 cgaagaactg gtcaagatgt acatcgagca gggcgtcgtc gctcgcgggg cgacggacga
      421 tgaaccctgg tctgtggctc tcgaccggct cgacgccgtc cgcgtgccgc cgaccctcgt
      481 cggcctcatc caggcccgcc tcgacgcgct gccggcggcc gaacgccgcg ccctgcaaca
      541 cgccgccgtc atcggccgcg tcttctggga ttcggctctg gcggcgctgg cgggcgcggc
      601 gaccccgccg ccgctgacca ccgcgctggg ccgcgaagtg gtgttccggc gcacgccttc
      661 cacctttgcc gactgccagg agttcctctt ccgccacgct ctggtgcgcg acgtggccta
      721 tgagaccgtg ctgctgagcg agcgccgcgc cctgcacgcc cacgccgcgc gctggctgga
      781 aacccacgcc gccgaaaggc tcgacgagtg tctcagcgtc ctggccgccc attgggacgc
      841 cgccaacgac gccacccgcg cggccgccta cctggtgcgc tcggccgagt tggcgctggc
      901 ggccggcaac cacggcgcgg cctgcgccac gttgcgccgg gcgttggagc taaccgcgcc
      961 ctcggcggcg acgctgttgc tgctgggcga ggcggcgttc atgatcagcg acttgcccac
     1021 cgccagcgac gccctggagc aggctctgaa tctggcccag acaaccggcg accgcgccgc
     1081 cgaagccaac agcctctact ggtatagccg cgtggccacg gcccgcgggg acttcgacac
     1141 ggctcgcgtc gcgctggaaa cggccctgcc cattgcccgc gccgtcggcg gcgcgacgct
     1201 ggcccgcgcc ctggaggggc tggccggggc caccggcagc cgtggcgatc tgcccgcggc
     1261 gacggcgctg ggcaaagagg ccctggcgct ggcccgcgag gtgggtgatg ccgtcctgga
     1321 gatgcgctgc ctggcgctgc tgggcacgtt tgcccggctg ggggacgacc tggactcggc
     1381 cgaggcgcgc ttccaggcag cgcgcgcgct ggcccaccag gccggcaatc tggagcgcga
     1441 ggccacggcc ctgctcaacc tgggcgacct ggcctatcgg cgcgagcgct acgacgacgc
     1501 ccgtgtcgcc gcccacgccg cccgcgacct cttccgcgac cagggtctgg ccgacgccgt
     1561 ggccatcgcc gccggcaacc tggcccaggc ggaactgatg ttgggcgagc gcgacgccgc
     1621 cgtggccgac gcccgccaag cgctggccgc cgcccgcgcc gccggggcca cgccgctggc
     1681 gctgttcgcc ctgtccgtct ttgcccaggc gctggccccc accgaccggc cgcgcgccgc
     1741 cgcgctgctg cacctggtca tccaccaccc ggccacggag tatcaggatc ggcgcgaggc
     1801 ggccgagttg ttgatgcacc tgggcacggc catcgttccg gcgacgccgc tagacctgga
     1861 cacgaccatc acagccatcc tcaacaacgc cgccagcgac tagagcaggt agcgcggcgc
     1921 gtccacgcgc cgcagttcgt cggccaggcg cagcttgaac aattccaact ggctcatcgg
     1981 ccgcttcagg ttgtagctct cgatctcctt gttgaggctg gcaatggtcg cctcccactt
     2041 ctggcaggaa tcatcccagc cgatggtcag ggccgagcgg tggctttccc ccggcgcggt
     2101 gaaggcggcg cgatagcgcc cccacgtgcg gctgatgtcg gcccgcagcg cgtcgatctt
     2161 gcccacgacc tccacccggc gcgtcagcca cggcgggcgc aggtcgttgt ccttcatgag
     2221 cttgttggtc agcgcggcgc tgccttcgta ggtgttggct tccaggtcga gcggccggcc
     2281 ctgcccggcc aggtcgtcga agaggccacg gcggcggccg tcctcgatca ggtcttcgat
     2341 caggtcgcgg aattcaaagc gcatgcggcg gcggtgttcc tcaccggcgg cgcgctcgcc
     2401 ctcgtctggt ggggtgtcga ttgggtcgtt catggggtga atgttagcgc caaaaagggc
     2461 gcggcgcacc cggaaagtgc gccgcgcccg tgcacgccgg tggtcgcgcc cggctcagtc
     2521 gatgcaggag tagttggccg tcgctccga
//
LOCUS       DAOSMQ010000477         2538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_40883_length_2538_cov_4.283931, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000477 DAOSMQ010000000
VERSION     DAOSMQ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40883_length_2538_cov_4.283931"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..839)
                     /locus_tag="PK829_13000"
     CDS             complement(135..839)
                     /locus_tag="PK829_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU42177.1"
                     /translation="MADPERIALVTGANRGIGREVSRQLARRGLRVILTARNAPAGQD
                     AADALAREGLPVVFCPLDVADAASVDAAKGWVEERFGRLDVLINNAAVYLDEQSNIFN
                     VPIADFTTTLAVNLYGPLYLCRAFVPGMRRRGYGRVVNVSSESGQLSTMGGYTPAYAI
                     SKAALNALTRVVAAEAGTSVKVNAACPGWVRTDMGGAHATRSVAEGAAGLVWLATLPD
                     SGPTGGFFRDGRPLDW"
     gene            complement(839..1846)
                     /locus_tag="PK829_13005"
     CDS             complement(839..1846)
                     /locus_tag="PK829_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU42178.1"
                     /translation="MPTVPTDHLDFFDGKRVLVTGGLGFIGSNLAHRLVSLGADVLLV
                     DSLIPEYGGNIFNVQDIENCLRINIADMRDEHGLRYLVQGQDVIFNLAGQVSHTDSMT
                     DPYTDLEINARSQLSLLEACRHGNPATKVVFASTRQIYGRPEYLPVDERHPLQPVDVN
                     GINKLAGEWYHMVYHEVYGLRTVSLRLTNTYGPRMRIRDARQTFIGWWFRQLLEGQTL
                     ALFGDGLQVRDFNYIDDVVEALLMVAATAAADGQIYNLGSDDPINLVNLARLLIEING
                     GGQYELRPFPADRKRIDIGDYYGDYRRIRSKLGWRPMVGLRDGMTRSLDYFRQYLEHY
                     I"
     gene            complement(1827..>2538)
                     /locus_tag="PK829_13010"
     CDS             complement(1827..>2538)
                     /locus_tag="PK829_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU42179.1"
                     /translation="TVLLTLRELTDDYEVIVVNDGSQDHTAAVLDELARVYPNEVRII
                     HHPQNRGYGGALRTGFYSAAKEWIFYTDGDAQYDPRELANLAALVSDDVDFINGWKIE
                     RNDPLHRIIIGRIYQSIIKAAFGLHLRDVDCDFRLMRREVFERIQLEADSGVICVELM
                     KKTQDAGFRLTETPVHHFHRAYGKSQFFRFRRLAQVARDLTRLWYRLVWRKEAAGPTS
                     SIEASAAPHPQTYADRSH"
BASE COUNT          439 a          783 c          881 g          435 t
ORIGIN      
        1 aaggcgctga cctccggccc caggatgtac cagccgccgg ccagcacgcg cgccgtcgcc
       61 gcgtcgattt cagcccgtag acgggcgtag gcgctcttca ggtcaaccag ggggatggcg
      121 ttggtcatgg ccgcttacca gtccagcggg cggccgtcgc gaaagaagcc gcccgtgggg
      181 ccggaatcgg gcagcgtggc cagccagacc aggccggccg cgccctcggc cacgctgcgc
      241 gtcgcgtgcg caccgcccat gtcggtgcgc acccagccag ggcaggcggc gttgaccttg
      301 accgacgtcc cggcttcggc ggcaacgacg cgcgtcaggg cgttgagggc ggccttggag
      361 atggcgtagg cgggcgtgta gccgcccatt gtgctcaatt ggcccgactc ggacgagacg
      421 ttgacaacgc gaccatagcc gcgccggcgc attccgggta caaaggcgcg acagaggtac
      481 agcgggccat agaggttgac ggccagcgtt gtcgtgaagt cggcgatcgg cacgttgaag
      541 atgttgctct gctcgtctag atagacggca gcgttgttga tgagcacatc caggcggccg
      601 aaacgctcct cgacccagcc tttggccgca tccacactgg cggcgtcggc cacatccagc
      661 gggcagaaaa cgacgggcag accctcgcgg gccagggcgt cggccgcgtc ctgcccggcc
      721 ggcgcgttgc gagcggtcag gatgacacgc aggccacgcc gggccagttg gcggctgacc
      781 tcccggccaa tgccgcggtt cgcgcccgtc accagggcaa tgcgttcggg gtcagccatc
      841 aaatgtagtg ctccagatat tgccggaagt agtcgagcga ccgcgtcatg ccatcgcgca
      901 ggccgaccat cggccgccag ccgagcttgc tgcggatgcg gcggtagtcg ccgtagtagt
      961 cgccgatgtc gatgcgcttg cggtcggccg gaaaggggcg cagctcatac tggccgccgc
     1021 cgttgatctc gatgagcagg cgggccagat tgaccaaatt gatggggtcg tcgctgccga
     1081 gattgtagat ttgcccgtcg gccgccgccg ttgccgcgac catcagcagc gcctcgacca
     1141 cgtcatcaat gtagttgaaa tcgcgcactt gcaagccgtc gccgaacagg gcgagcgtct
     1201 ggccctccag cagttgccgg aaccaccagc cgatgaacgt ttgccgcgcg tcacggatgc
     1261 gcatccgggg gccgtatgtg ttggtcaggc gcagggagac ggtgcgcaga ccatagacct
     1321 cgtggtagac catgtgatac cactcgccgg ccagcttgtt gatgccgttg acgtcgaccg
     1381 gttgcagggg atggcgctca tcgacgggca ggtactccgg gcggccgtag atctgccgcg
     1441 tgctggcaaa gacgaccttc gtggccgggt tgccgtggcg acaggcttcc agaagcgata
     1501 gctggctgcg ggcgttgatc tccagatcgg tgtacggatc ggtcatgctg tcagtgtggc
     1561 tgacctgacc ggccaggttg aagatcacgt cctgcccttg caccagatag cgcagcccgt
     1621 gctcgtcgcg catgtcggca atgttgatgc gcaggcagtt ttcaatgtct tggacgttga
     1681 agatgttgcc gccgtactcc ggaatgagcg aatcgaccag caacacgtcg gcccccaggc
     1741 tcaccagccg gtgggccagg ttggaaccga tgaagcccag cccgccggtg accaggaccc
     1801 gcttgccgtc gaagaagtcg agatggtcag tgggaacggt cggcataggt ctgagggtga
     1861 ggggcggcgc tcgcttcgat ggacgaagtc ggcccggcgg cttccttgcg ccacaccagg
     1921 cggtaccaca gcctggtcag gtcgcgcgcc acctgggcca ggcggcggaa gcggaagaac
     1981 tgcgacttgc cgtaggcgcg atgaaagtgg tggacgggcg tctcggtcag gcgaaagccc
     2041 gcgtcctggg ttttcttcat cagttcgacg cagataacgc cgctgtcggc ctccaactgg
     2101 atgcgctcga agacctcgcg gcgcatcaga cggaagtcgc aatcgacatc gcgcagatgg
     2161 aggccgaaag cggccttgat gatggactgg tagatgcggc cgatgatgat gcggtgcagc
     2221 ggatcgttgc gctcgatctt ccagccgttg atgaaatcga cgtcgtcgct gaccagcgcg
     2281 gccaggttgg ccaactcgcg cgggtcatac tgcgcgtcgc cgtcggtgta gaagatccac
     2341 tccttggccg cactgtagaa gccggtgcgc agcgccccgc catagccgcg attctgcgga
     2401 tggtggatga tgcgcacctc gttggggtag acgcgcgcca gttcatcgag cacggcggcg
     2461 gtgtggtcct ggctgccgtc gttgacgacg atgacctcat agtcatcggt cagttcgcgc
     2521 agcgtcagga gcacggtg
//
LOCUS       DAOSMQ010000478         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_41328_length_2517_cov_3.580829, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000478 DAOSMQ010000000
VERSION     DAOSMQ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41328_length_2517_cov_3.580829"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PK829_13015"
     CDS             <1..1241
                     /locus_tag="PK829_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOU42180.1"
                     /translation="TRFDSWLDMRCQPYIAWLNDHHADLITRLTGCAPTCDHGPKILW
                     WKGERPDDYARIAKFLTPSAYVAGRMAGLSAGQAFMDYTFIHFSGLSDNRAGTWSTAI
                     CDTLGVDMGKLPAIVEPWRVIGEVSEVAARDFGLAAGTPIAAGCGDTAANALGAGIVR
                     PGQVLDVAGTASVLAGCTDAFVADTTHRALLTMRSVIPGLWNPLAYIAGGGQALRWFR
                     DEFAGGTHGGDADAAYAAMIAAAAAAPPGAEGLFFSPHLGGRICPATPELRGAWLGFS
                     FGHTQAHFLRAVLESVAYEYAWYLRVLREYIPNLELTETRAVGGGARSDAWNQIKADV
                     LDVPYQRLARTEFGTWGSALIAGKAVGIYDDLAATAAEHARPAGPPLRPSAAAHERYR
                     PLVDRHIRLQEQLVTMFTSA"
     gene            1307..2347
                     /locus_tag="PK829_13020"
     CDS             1307..2347
                     /locus_tag="PK829_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU42181.1"
                     /translation="MPSPIRICMIGAGRVGKLHTGTLTRHVPGGQVVALVDPWADVLK
                     ATGDLFGIERRFASLADALAAVAFDAVVITTPTPTHRELAVLAAEQGKHVFLEKPMAL
                     SLPECDAILDAARRHAVILQMGFMRRFDPDFVAAWERIQGGEIGEPTMIKSLTHGPGL
                     PPPWATDLKTSNGNLAEVNSHDLDTLRWLMASDPRRIYVEVANFKGAERGVTTEHYYD
                     NMFATVKFESGALGNISGVCPCDYGYDSRVEIIGKKGIMHIGRMEGEAIVVCTDRDHG
                     LVTPIFRRWPDRFRWGYIQELEHFVECIQRGRTPRVGGEDGRWAVAMVVAGTRSFLEE
                     RPVYLRETGVSQ"
BASE COUNT          377 a          885 c          864 g          391 t
ORIGIN      
        1 cgacgcgctt cgattcgtgg cttgacatgc gctgccagcc ctacatcgcc tggctcaacg
       61 accaccacgc cgaccttatt acccggctca ccggctgcgc gccgacgtgc gaccacggcc
      121 cgaagatcct gtggtggaag ggggagcggc cggacgacta cgcccgcatc gccaagttcc
      181 tgacgccctc ggcctacgtc gcggggcgca tggccgggct gagcgctggc caggccttca
      241 tggactacac cttcatccac ttctccggcc tgtcggacaa ccgcgccggg acgtggagca
      301 cggccatctg tgacacgctg ggcgtggaca tgggcaaact gccggccatc gtcgagccgt
      361 ggcgggtgat cggcgaagtg agcgaggtag cggcgcgcga cttcggcctg gccgcgggta
      421 cgcccatcgc cgccggctgt ggcgacacgg ccgccaacgc tcttggcgcg ggcatcgtcc
      481 ggccggggca ggtgctcgac gtggccggca cggcgtcggt gctggccggc tgcaccgacg
      541 ccttcgtggc cgacacgacc caccgggcgc tgttgaccat gcgctcggtc atccccggcc
      601 tgtggaaccc gctggcctac atcgccgggg gcgggcaggc gctgcgctgg ttccgcgatg
      661 agtttgccgg cgggacacac ggcggcgacg ccgacgcggc ttacgcggcc atgatcgccg
      721 ccgccgccgc cgcgccgccg ggggccgagg ggctgttctt ctcgccccac ctgggcgggc
      781 gcatctgtcc ggcgacgccg gagttgcgcg gcgcgtggct gggcttctcg ttcggccaca
      841 cgcaggccca tttcctgcgg gccgtgctgg agagcgtcgc ctatgagtat gcctggtatt
      901 tgcgggtgct gcgcgagtac atccccaacc tggaactgac cgaaacgcgc gccgtcggcg
      961 gtggggcgcg cagcgacgcc tggaaccaga tcaaggccga cgtgctggac gtgccctacc
     1021 aacggctggc gcgaaccgag ttcggcacgt ggggcagcgc gctcatcgcc ggcaaggccg
     1081 tgggcatcta tgacgacctg gcggccacgg cggccgagca cgcccgcccg gccggcccgc
     1141 ccctgcgccc ctcggcggcg gcgcacgaac gctaccggcc gctggtggat cggcacattc
     1201 gcctgcaaga gcaactggtg acgatgttta cgagcgcgta ggggcggcgc ggccgtcctt
     1261 cgccggcggg gcgcgccccg ccccgttgag gagagagaat tagcctatgc cctcacccat
     1321 tcgcatctgc atgatcggcg ccgggcgcgt cggcaagctg cacacaggca cgctgactcg
     1381 ccacgtgccc ggcggccagg tcgtcgccct ggtcgatccc tgggccgatg tgctcaaggc
     1441 cacgggcgac ctgttcggta tcgagcggcg cttcgcctcg ctggccgacg cgctggcggc
     1501 ggtggccttc gatgccgtcg tcatcaccac gccgacgccc acccaccgcg agctggccgt
     1561 gttggccgcc gagcagggca agcacgtctt cctggagaag ccgatggctc tgtcgctgcc
     1621 ggagtgcgac gccatccttg acgctgcccg ccgccacgcc gtgatcttgc agatgggctt
     1681 catgcgccgc ttcgaccccg acttcgtggc tgcctgggag cgcatccagg gtggcgagat
     1741 cggcgaaccg acgatgatca agtccctcac ccacggcccc ggcctgccgc cgccgtgggc
     1801 cactgacctg aagaccagca acggcaatct ggccgaggtc aacagccatg atctggacac
     1861 cctgcgctgg ctgatggcgt cggacccgcg gcgaatctac gtcgaggtgg ccaacttcaa
     1921 aggggccgag cgcggcgtga ccaccgagca ctactacgac aatatgttcg ccacggtcaa
     1981 gttcgagtct ggcgcgctgg gcaacatctc cggcgtctgc ccgtgcgact acggctacga
     2041 cagccgcgtg gagatcatcg gcaagaaggg catcatgcac atcggccgca tggagggcga
     2101 agccatcgtc gtctgcaccg accgcgacca cggcctggtg acgcccatct tccgccgctg
     2161 gcccgatcgt ttccgctggg gctacatcca ggagctagag cacttcgtcg agtgcatcca
     2221 gcgcggacgg acaccgcgcg tgggtggcga agacggccgt tgggccgtgg cgatggttgt
     2281 ggccggcacg cggtcgttcc tggaggagcg gccggtctat cttcgcgaga cgggcgtcag
     2341 ccaataggcg cgccgggcgt gcccgcgttc cacgcgcgca accactttcg acatcactga
     2401 cccacggaac gacccacgga gacacaccat gaaccgcgat tttgatccga cactgtttga
     2461 agaggttctg gccgcggggg ccattgctgg caatgtgctg ctcagcccgc tgacccg
//
LOCUS       DAOSMQ010000479         2506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_41569_length_2506_cov_4.789474, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000479 DAOSMQ010000000
VERSION     DAOSMQ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41569_length_2506_cov_4.789474"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PK829_13025"
     CDS             <1..393
                     /locus_tag="PK829_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU42182.1"
                     /translation="VSDAEALARDTHKFESRYNHSLIGPYPARRDLYVERSPIYVAPD
                     CSAALILFQGLEDKVVPPDQSAAMFIAVRDKGLPVAYVAFEGEGHGFRQAANIKRAME
                     AELYFYGRVFGFEPAEQLEPVVIENLHV"
     gene            complement(424..>2506)
                     /locus_tag="PK829_13030"
     CDS             complement(424..>2506)
                     /locus_tag="PK829_13030"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HOU42183.1"
                     /translation="AAAAGDDNALAGARGLAQGAIYWGGYTAARAALAAGDAETAGQW
                     LLVRDFRPSTRFSRPDADATLALRALADGATAGAPTAGATTTALNQLDADLLDTYQAR
                     LAEHLATAADPTAQRGRRAEAAGLAAATWRVLAPAYAEQTAADPAAMTATVDGLLPAL
                     LAGDETAVAATTARLGEALASFRAAPLSPDDQARRAGQLLRYLTLVPVEYGRGVTERT
                     VMIDLEVQEAVTFMRGAEAAFADLRLDLAAIDPTQTNALAAQIAAAVADLDAANHKTG
                     VLTPDEMAANVEAITAGLTALFPPEWTTNADADFDVLASVLDQMEAAIGAGDYTLAES
                     ARLEAYAIFDFGPEPRLLGFAPPLVARIDGLFWQGYGGQTGLAQAIALGAAPAEIAAI
                     RVDLDSALEEAQLTLGDLPSAPGAIISNAAIIVFREGLESVVILAALMASMVGALARH
                     RRPMVLGVVLSLLATAATWWIAQEILSSLTRFGERLEAVVSLIAIGILLLITNWFFHK
                     AYWKDWMAGFHQQKRRLLSAETGQFVGLVILGFTSMYREGFETVLFLQALVLDAGTAV
                     VLEGVAVGALAVLVVGLVTFKLQARLPYKKMLVWTGVLIGLVLVTMVGKTVHVLQAVG
                     WLGISPIAGLTLPYWSGLWFGLYATWQGLIAQISAALFVLGSYYLAEGMQQKRRKNLP
                     VARAERAQPNP"
BASE COUNT          388 a          872 c          896 g          350 t
ORIGIN      
        1 gtcagcgacg ccgaggcgct ggcccgcgac acgcacaagt tcgagtcgcg ctacaaccac
       61 agtctcatcg gtccctaccc ggcccgccgc gacctgtacg ttgagcgctc gcccatctac
      121 gtggcccccg attgcagcgc ggcgctgatt ctctttcagg ggctggagga caaggtcgtg
      181 ccgccggatc agtcggcggc catgttcatc gccgtgcgcg acaaggggct gcctgtcgcc
      241 tatgttgcct ttgagggcga ggggcacggc ttccggcagg cggctaacat caagcgggcg
      301 atggaggcgg agctgtactt ctacggtcgc gtcttcggct tcgagccggc cgagcagttg
      361 gagccggtgg tgatcgaaaa cctgcacgtg tagggcgggt tgccgaaccc gcccggcggg
      421 tgttcacggg ttcggctgcg cccgctcggc gcgggccacg ggcaggttct tgcggcgctt
      481 ctgctgcatg ccctcggcca ggtagtagct gcccagcaca aagagggccg cgctgatctg
      541 ggcgatgaga ccctgccacg tggcgtagag gccgaaccac agccccgacc agtagggcag
      601 cgtcagcccg gcaatgggcg agatgcccag ccagccgacg gcctgcaaca cgtgcaccgt
      661 cttgcccacc atcgtcacca gcaccaggcc gatgagcacg cccgtccaga cgagcatctt
      721 cttgtaaggc aggcgcgcct ggagcttgaa ggtgaccagg ccgacgacca gcacggccag
      781 tgcgccgacg gctacccctt cgagcacgac ggccgtgccc gcgtcgagca ccaacgcttg
      841 caggaacagc accgtctcga agccctcgcg gtacatgctg gtgaagccga ggatgaccag
      901 gccgacgaac tggcccgtct cggccgagag cagacgccgc ttctgctggt ggaagccggc
      961 catccagtcc ttccagtagg ctttgtggaa gaaccagttg gtgatcagca acaggatgcc
     1021 gatagcgatg agggacacga ccgcctccag ccgctcacca aagcgcgtca gcgacgacag
     1081 aatctcctgg gcgatccacc acgttgctgc cgtggccaac agcgacagga cgacgcccag
     1141 gaccatcggc cggcggtggc gggccagcgc cccgaccatg ctggccatca gcgccgccag
     1201 gatgacgacc gactccaacc cctcgcggaa gacgataatg gccgcgttgg agatgatcgc
     1261 ccccggcgcg ctgggcaggt cgcccagggt cagttgcgct tcctccagtg cgctgtccag
     1321 atcgacgcga atggcggcga tctccgccgg cgcggccccc aaagcgatgg cctgggccag
     1381 gcccgtctgg ccgccgtatc cctgccagaa gaggccgtcg atgcgggcca ccagcggcgg
     1441 ggcgaagccc agcagccgcg gctccggccc aaagtcgaag atggcgtagg cctccaggcg
     1501 ggccgactcg gccagggtat agtccccagc gccgatggcg gcctccatct ggtcgagcac
     1561 ggaggccagc acgtcgaaat cagcgtcggc gttggtcgtc cactctggcg ggaagagggc
     1621 cgtcagcccg gcggtgatag cctcaacgtt ggccgccatc tcgtcgggcg tcagcacgcc
     1681 ggtcttgtga ttggcggcgt ccagatcagc cacggccgcg gcgatctgcg cggccagcgc
     1741 gttggtctgg gtggggtcaa tagccgccag gtcgaggcgc aggtcggcga aggcggcctc
     1801 ggccccgcgc atgaaggtga cggcctcctg cacctccagg tcgatcatca ccgtccgctc
     1861 tgtcacccca cggccgtact ccaccggcac tagggtcaga tagcgcagca gttggccggc
     1921 gcggcgggcc tggtcgtcgg gcgagagggg cgcggcgcgg aaggaggcca gcgcctcgcc
     1981 cagacgcgct gtcgtcgccg ccacggccgt ctcgtcgccg gccagcagcg ccggcagcag
     2041 cccgtccacc gtggcagtca tcgcggccgg gtcggcagcc gtctgctcgg cgtaggcggg
     2101 ggccaggacg cgccaggtgg ccgcggccag accggctgcc tcggcgcggc ggccgcgttg
     2161 ggcagtgggg tcggccgccg tggccaggtg ctcggccaga cgggcttgat aggtgtccaa
     2221 caagtcggcg tccagttggt tcagcgcggt ggtagtggcg ccagccgtgg gcgcgccggc
     2281 cgtggcccca tcggccagcg cacgcaaggc cagcgtcgcg tcggcgtcgg ggcgggagaa
     2341 gcgcgtgctg gggcggaagt cgcgcaccag cagccactgc ccggccgtct cggcatcgcc
     2401 cgccgccagg gccgcgcggg cggcggtgta gccgccccag tagatcgccc cctgggccag
     2461 gccgcgcgcg ccggccagcg cgttgtcgtc gccggcggcg gcggcc
//
LOCUS       DAOSMQ010000480         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_41689_length_2501_cov_5.668438, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000480 DAOSMQ010000000
VERSION     DAOSMQ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41689_length_2501_cov_5.668438"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PK829_13035"
     CDS             complement(<1..242)
                     /locus_tag="PK829_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HOU42184.1"
                     /translation="MSDSDALKRAAAERAVNYIQSGMVVGLGTGSTAVFAVRRIGALL
                     AAGELQGIVGIPTAEVTAREAERCGVPLGSLDDYPAV"
     gene            complement(235..2256)
                     /locus_tag="PK829_13040"
     CDS             complement(235..2256)
                     /locus_tag="PK829_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOU42185.1"
                     /translation="MAIDFGREVCGDLATATRREWLVTNGIGGFASGTVANALSRRYH
                     GLLTAALHPPLGRTLLLAKLDETAEYDGVYPNSGRLTPLYVNRWEDSAVEGNGHHAIN
                     RFHLEGTTPVWTYAVANALLEKRIWMQPGANTTYVQYHLTRATGPLGLTIKALANYRD
                     YHSTTIVNDWSPAVEPVARGVRITMFDGARPFYLLSDRAAITPQFDWYEDFFLAIEEQ
                     RGQRDVTEDHLYLAQLQVLLRPGERFIVVASTEPSPHMNGDEALAERRAHEAALLERG
                     ETTGAGDAWRQLVLAADQFIVNRPTPGDPDGKSIIAGYHWFSDWGRDTMIALPGLTLA
                     TGRPEVAASILRTFAHFVDQGMLSNRFPDAGETPEYNTVDATLWYFVALHETIAATGD
                     TALARELFPVLAGIIDWHIRGTRYNIHVDPADGLLFAGEAGVQLTWMDVKVDDWVVTP
                     RIGKPVEINALWYNALRIMADLAGRLGEDAGQYAALAERVRAGFERFWNAPLGYCFDV
                     IDGPAGIADVVAGNDGRLRPNQLLAVSLPYSPLPAERQRSIVDACGRHLLTAHGLRSL
                     SPDDPGYIGRYGGDRRKRDGAYHQGTVWGWLIGPFALAHLRVYGDRAAARSYLEPLLQ
                     HLNDHGLGSISEIFEGDAPFAPRGCIAQAWSVAEALRVWQATAERGDNE"
BASE COUNT          372 a          818 c          897 g          414 t
ORIGIN      
        1 acggcggggt aatcgtcgag gctgcccagg ggcacgccgc agcgctcggc ctcgcgggcg
       61 gtcacttcgg ccgtggggat gccgacgatg ccctggagtt cccccgcggc cagcagcgcg
      121 ccgatgcgcc ggacggcgaa gacggccgtg ctgcccgtgc ccaacccgac gaccatgccc
      181 gactgaatat agttgacggc gcgttcggcc gccgcccgct tcaatgcatc gctatcactc
      241 attgtcgcct cgctcggccg tcgcctgcca gacgcgcaac gcctcggcca cgctccacgc
      301 ctgggcgatg cagccgcgcg gggcgaaggg cgcgtcgccc tcgaagatct cgctgatgct
      361 gcccagaccg tggtcgttca gatgctgcaa cagtggctcc agatagctgc gcgccgcggc
      421 cctgtcgccg tagacgcgca gatgggccag ggcgaacggg ccgatgagcc agccccagac
      481 cgtgccctga tgataggccc cgtcgcgctt gcgccggtcg ccgccatagc ggccgatgta
      541 gccggggtcg tcgggcgaga gggagcgcag gccgtgggcg gtcagcaggt ggcggccgca
      601 ggcgtcgacg atagagcgct gccgctccgc tggcagcggg ctatagggta gtgagacggc
      661 cagtagctga ttggggcgta ggcggccgtc gttgccggcg acgacatcgg cgatgcctgc
      721 cggcccgtcg atgacgtcga agcagtagcc cagcggcgcg ttccaaaagc gctcaaaccc
      781 ggccctgacc cgctcggcca gcgcagcgta ctggccggcg tcctcgccca gccgcccggc
      841 caggtcggcc atgatgcgca gggcgttgta ccacagggcg ttgatctcca ccggcttgcc
      901 gatgcgcggc gtcaccaccc agtcatcgac cttgacgtcc atccacgtta gctgcacgcc
      961 cgcctcgccg gcaaaaagca ggccgtcggc cgggtccacg tggatgttgt agcgcgtgcc
     1021 gcggatgtgc cagtcgatga tgccggccag cacggggaac agttcgcggg ccagggccgt
     1081 gtcgcccgtg gcggcgatgg tctcatgcag ggcgacgaag taccacagcg tcgcgtcaac
     1141 cgtgttgtac tccggcgtct cgcccgcgtc ggggaagcga ttggacaaca tgccctgatc
     1201 gacgaagtgg gcgaaggtgc gcaggatgct ggcggctact tccggccggc cggtggccag
     1261 cgtcagcccc ggcagggcga tcatcgtgtc gcggccccaa tcgctgaacc agtggtagcc
     1321 ggcgatgatc gacttgccgt cggggtcgcc cggcgtcggc cggttgacga tgaactgatc
     1381 ggcggccaaa accagttgcc gccaggcgtc gccggccccg gtcgtctcac cgcgctccag
     1441 cagggccgcc tcgtgggcgc gccgctcggc caacgcctcg tcgccgttca tgtgcggcga
     1501 cggctccgtg ctggccacga cgatgaagcg ctcgcccggc cgcagcagca cttgcagttg
     1561 ggccagatag aggtggtctt cggtcacgtc gcgctggccg cgttgctcct cgatggccag
     1621 gaagaagtcc tcgtaccagt cgaactgggg ggtgatggcc gcccggtcgc tgagcaggta
     1681 gaacggccgc gcgccgtcga acatggtgat gcgcacgccg cgcgcgacgg gttcgaccgc
     1741 gggcgaccag tcgttgacga tggtcgtgct gtggtagtcg cggtagttgg ccagcgcctt
     1801 gatggtcagg cccagcgggc cggtggcgcg ggtcaggtga tactggacgt aggtggtgtt
     1861 ggcccccggc tgcatccaga tgcgcttttc cagcagggcg ttggccaccg cgtaggtcca
     1921 caccggcgtc gtgccttcca gatggaagcg gttgatggcg tggtggccgt tgccctcgac
     1981 ggcgctatct tcccagcggt tgacgtaaag tggggtcagc cggccgctgt tggggtaaac
     2041 gccgtcgtac tcggccgtct cgtcgagctt ggccagcagc agcgtgcgcc ccagcggcgg
     2101 gtgcagcgcc gccgtcagca ggccgtggta gcggcgcgag agggcgttgg ccaccgtgcc
     2161 ggaggcgaag ccgccgatgc cgttggtcac cagccattcg cgccgggtcg ctgtcgccag
     2221 gtcgccgcac acctcgcggc cgaagtcaat tgccataggt gattcactcc gtcggtttgt
     2281 cggcgggtac tatagcggga ttcaggccgg tgcgcatcct tgggccagag gcaggtaggg
     2341 ttattcagaa gtcgcaccgc cgtagcacag cctgttaggc tgtggctcct gttaggctgt
     2401 ggctcctgtt aggctgtgac gcctgttagg ctgtggctgg cgaagacagc ctgacaccct
     2461 aattttgcgc aaaaatcggg gggtatcaaa ggtccaccga g
//
LOCUS       DAOSMQ010000481         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_41697_length_2501_cov_3.614881, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000481 DAOSMQ010000000
VERSION     DAOSMQ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41697_length_2501_cov_3.614881"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1344)
                     /locus_tag="PK829_13045"
     CDS             complement(58..1344)
                     /locus_tag="PK829_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU42186.1"
                     /translation="MPLWMTSEDESKPAVERCARCDSILPEGAERCLMCGLPRPPRAG
                     PTEIVAEPSPTLATFVSPLASVEQVAPSPPPAFVDYPVRESRSTRLFWLVATLVIIGL
                     TAMWLALRDDGPQVMAAFIPTTTPLPPTITSTPTWTPLPSETVPPSVTPSPSTTPAPT
                     STPRDPRAHTVAAGETLFGLSLLYRISAESIAQANNIDLNTSIQSGQSLVIPWPTATP
                     PLESVLLTINGEQVMADATNCEIITIQSGDSAYGLSAVKGVPLEAIIAVNRQTQESIQ
                     LLQPGDTLCIPRILYGDTIPPTAGPSPTPSLTPPPAGPALLNPPNGASFGVTDEPIVL
                     QWLAVKDLAADEWYMVELRDADDRDSLPHRGFTRDQSFRVPAEWRPAAGGARQIEWRV
                     SIVQVTGRRSDGGFIYAFRGTAGAVAYFIWQGAATP"
     gene            1470..2471
                     /locus_tag="PK829_13050"
     CDS             1470..2471
                     /locus_tag="PK829_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOU42187.1"
                     /translation="MADLTTSYLGLTLKSPIVVSSNPLTYDVNNIRRMEEAGAGAVVL
                     YSLFEEQIELAEIGFSNYYEQHREELPEALRHVAEMKEYNQGAGSYLAHLYQVKHATA
                     MPVIASLNGYYSSGWIRYARLIEAAGADALELNIYYLATKPTVSGAEVEQMYTDLVRD
                     IKQSIRIPIAVKLNPYFSAVANISRRLVSAGAHGLVLFNRFYQPDFDIENRAIVPSLE
                     LSTPSELRLRLRWVALLAGQVNADLAITGGVHSAEDVIKGLMAGASVTMMASALIQHG
                     IDHLAAVTTNLQSWLDSHAYASVSDIRGCLSHVQIGDSAALERANYLNVLRTARAKD"
BASE COUNT          441 a          786 c          852 g          422 t
ORIGIN      
        1 ggagcgccgg cagaatccgt ttctgaccga tttgacgact gaggagtaag cagggtgcta
       61 gggggtcgcc gcgccctgcc aaatgaaata cgccactgcc ccggccgtcc cccggaaagc
      121 gtaaatgaag ccgccatcgg agcgccgccc ggtcacctgg acaatgctga cgcgccactc
      181 gatttggcgc gcgccgccgg ccgctggccg ccactcggcc gggacgcgga acgactggtc
      241 gcgggtgaag ccgcggtggg gcaggctgtc gcggtcgtcg gcgtcgcgca gttcgaccat
      301 gtaccactcg tcggcggcca ggtccttgac cgccagccat tgcaggacga tcggctcatc
      361 cgtcacgccg aagctggccc cgttgggggg attgagcagc gccgggccgg ctggcggcgg
      421 cgtcagcgag ggcgtcggcg aggggccggc cgtgggtggg atggtgtcgc cgtagaggat
      481 acgcgggatg cacagcgtgt cgcccggctg caagagttgg atcgactcct gcgtctgccg
      541 gttgacggcg atgatggctt ccaggggcac gcccttgacg gcggagaggc cataggccga
      601 gtcgcccgac tggatggtga tgatctcgca gttggtggcg tcagccatga cctgttcgcc
      661 attgatggtc agcaggactg actccagcgg cggcgtggcc gttggccagg ggatgacgag
      721 gctctggccg gactggatgg acgtgttgag atcgatattg ttggcctggg cgatgctctc
      781 ggccgagatg cgatagagca gcgacagacc gaagagcgtc tccccggcgg cgacggtgtg
      841 ggcgcgcggg tcgcgcggcg tgctggtcgg cgcgggcgtg gtcgaggggc tgggcgtgac
      901 cgacggcggg acagtctccg acggcaaggg tgtccacgtc ggcgtgctgg tgatggttgg
      961 cggcagaggc gtcgtcgtgg ggatgaaggc ggccatgacc tgcggcccgt cgtcgcgcag
     1021 cgccagccac atcgccgtca ggccgatgat gaccaacgtc gccaccagcc agaacaggcg
     1081 cgtcgagcgg ctttcgcgca cgggataatc gacgaaggcc ggcgggggag agggcgcaac
     1141 ctgctcgacg gacgcgagcg ggctaacaaa tgtcgccagc gtgggactcg gctcagcgac
     1201 gatttctgtc ggcccggcgc gcggcggccg cggcaggccg cacatcaggc agcgttcggc
     1261 tccttcgggc agaatactat cgcagcgggc gcagcgttcg accgccggct tgctctcgtc
     1321 ctcgctcgtc atccagaggg gcattatagc ggcgcggttg actggcctca accggtgtaa
     1381 cggcgcgctc ataatcctcg ttcggccgca tttctgctac aatagggatg atcatagcgg
     1441 acatcaccct cgcgagtgga atcaatctaa tggctgacct gactacttcc tacctgggcc
     1501 taaccctcaa atccccaatt gtggtatcgt cgaacccgct gacctacgac gttaataaca
     1561 tccgtcgaat ggaggaggcc ggtgcgggcg cggtcgtgct ttactcgctg ttcgaagagc
     1621 agattgaact ggcggagatt ggctttagca actactacga gcagcaccgc gaggagctgc
     1681 cggaggcact gcgccacgtg gccgagatga aagagtataa ccagggcgcg ggcagctacc
     1741 tggctcatct ctaccaggtc aaacatgcca cggccatgcc cgtcatcgcc agcctgaacg
     1801 gctactacag cagcggctgg attcgctacg cgcgtctcat cgaggccgcc ggggccgatg
     1861 ccctggaact gaatatctac tacctggcca ccaagcccac cgtttctggc gccgaggtgg
     1921 aacagatgta caccgacctc gtgcgcgaca tcaagcagtc gatccgcatc cccatcgccg
     1981 tgaagctgaa cccctacttc agcgccgtgg ccaacatctc ccggcggctg gtgagtgccg
     2041 gggcgcacgg gctggtactg ttcaaccgct tctaccagcc cgacttcgac attgagaacc
     2101 gcgccatcgt gcccagcctg gaattgagca cgcccagcga gttgcgcctg cgcctgcgct
     2161 gggtggcatt gctggccggg caggtcaacg ccgacctggc cattaccggc ggtgtccaca
     2221 gcgctgagga tgtcattaaa gggctgatgg ccggggccag cgtgacgatg atggcctccg
     2281 cgctcatcca gcacggcatc gaccacctgg ccgcggtgac gaccaacctg caaagctggc
     2341 ttgacagcca cgcctatgcc tccgtcagcg acatccgcgg ctgcctgagc cacgttcaga
     2401 ttggcgactc ggccgcgttg gagcgggcca actatctcaa cgtcctgcgc acggcgcgcg
     2461 ccaaggactg aggccactag ccacaactat gatcactctg c
//
LOCUS       DAOSMQ010000482         2495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_41821_length_2495_cov_5.095902, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000482 DAOSMQ010000000
VERSION     DAOSMQ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41821_length_2495_cov_5.095902"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PK829_13055"
     CDS             complement(<1..770)
                     /locus_tag="PK829_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOU42188.1"
                     /translation="MRIGILSRNPALYSTRRLAQAIWARGHQAQIIDTTAIAVHLGTG
                     ETPPVLTANAIGLAVTSPLPPLDAVIPRIGTSVTFYGLAVVRQFEAAGVFSAASSDAI
                     ARSRDKLHSLQVMAQAGLPIPRTAVVARPEALLAAVRAVGGLPAVVKLIRGTQGRGVL
                     LADHIATIAAMLRRVEELNQQAIIQEYIAEAAGRDLRIIVVGSRPVAAMERHAPPGDF
                     RANLHQGGSAEPASLDHATAALAVAAARAHGLAVAGVD"
     gene            878..1660
                     /locus_tag="PK829_13060"
     CDS             878..1660
                     /locus_tag="PK829_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOU42189.1"
                     /translation="MPIDTFVIVPTYNEADNLDDLLSQLLALPVTLGAVVVDDNSPDG
                     TGALADRWAARQPGRITVVHRPAKLGLGTAYIAGFQKALFELGAERVLTMDADFSHNP
                     RYIPALLALSQHVHVAIGSRYAPGGGTRNCTWQRIFLSRLANGVARTLLGLRARDATA
                     GFRLYRREVLLSIPLDEIFSSGYSFLIEMLFLCQRRGWQIGETPIIFEDRRKGTTKIS
                     RQEIFKAQYTVFRLFLRRLRGGEPRRPSISVSPAINNDHRSL"
     gene            1641..>2495
                     /locus_tag="PK829_13065"
     CDS             1641..>2495
                     /locus_tag="PK829_13065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU42190.1"
                     /translation="MITVPCNLCGRDDYRVRFPATTEPSALRADAFRCTHSGYGHHLQ
                     IVECCHCGLVYANPRWPTDTVLEAYTSVEDETYVQERHGRELTFRRHLRRIERIIGPA
                     QGRTLLDVGAYIGVFVEVAAAAGWRAQGVEPSAWAAAEAQRRGLAVHDGTLASVDLPP
                     ESFDVITMWDVIEHLSDPAAELARARRLLRPGGWLVAHTMDIGAPLARIMGARWPWLM
                     DMHLYYFDRRTLPAMFTANGLRVVWQGTQGRYLRLGYLASRVGGLNAGLGRLAGAVVN
                     GLGLPAAAV"
BASE COUNT          363 a          851 c          847 g          434 t
ORIGIN      
        1 aagtccaccc cggcgacggc cagcccgtgg gcgcgcgcgg cggccacggc cagcgccgcc
       61 gtggcgtggt cgagcgacgc cggctcggcc gagccgccct gatggagatt ggcgcggaaa
      121 tcgcccggcg gcgcgtgccg ctccatagct gccaccggtc ggctgccgac gacgatgatc
      181 cgtaagtcgc gcccggccgc ctcggcgatg tactcctgaa tgatcgcctg ctggttcaac
      241 tcctccaccc gtcgcagcat cgccgcgatg gtggcaatat ggtcggccaa cagaacgccg
      301 cgcccctgcg taccccggat aagcttaacc accgccggca gcccgccgac agccctgaca
      361 gcggccagca gcgcctccgg ccgggcgacg acggccgtgc gcgggatggg caagcccgcc
      421 tgggccatga cttgcaggct gtggagcttg tcgcgcgagc gggcgatggc gtcagacgac
      481 gccgcgctga agacgccggc cgcctcgaac tggcgcacga cggccagccc gtagaacgtc
      541 accgacgtgc cgatgcgagg aatgacggca tcaagcggcg gcagcggcga cgtgaccgcc
      601 aggccgatgg cgttggccgt cagcacgggc ggcgtctcgc ccgtgcccag gtggacggcg
      661 atagcggtgg tgtcaatgat ctgcgcctgg tggccgcgcg cccagatggc ctgggccagc
      721 cggcgggtgc tatagagcgc tggattgcgg gagaggatgc cgatgcgcat gcagcttatt
      781 gtcccctgtc gtccagccgc tggccaatga acgtgagctt acccgcgcgt tgccctatcc
      841 tgcccttgcc gctacaatct ccctcggagc gccgtccatg cccattgata ccttcgtgat
      901 cgtgcccacc tacaacgagg ccgacaatct ggatgatctg ctgagccaac tgctggccct
      961 gccggtgacg ttgggcgcgg tggtggtcga tgacaattcg cccgacggca cgggcgcgct
     1021 ggccgaccgc tgggccgcgc ggcagccggg gcgcatcacc gtcgtccatc gtccggccaa
     1081 gttgggtctg ggcacggcct atatcgccgg ctttcagaag gctctgttcg agttgggcgc
     1141 tgagcgggtg ctgaccatgg acgccgattt ctcccacaac ccacgctaca ttcccgcctt
     1201 gctcgccctg agccagcatg tccacgtggc catcggttcg cgctatgcgc ccggcggcgg
     1261 cacgcgcaac tgtacctggc agcgcatctt tctgagtcgc ctggccaacg gcgtggcgcg
     1321 gacgctgctg gggctacggg cgcgcgacgc tacggctggc ttccgtctct accggcgcga
     1381 ggtgctgctg tccattccct tggacgaaat cttctccagc ggctattcgt tcctgatcga
     1441 gatgctcttc ctctgccagc ggcgtggttg gcagatcggc gaaacgccca ttattttcga
     1501 agatcggcgc aaggggacaa cgaaaatttc gcgccaggag attttcaagg cccaatacac
     1561 tgtctttcgc ctgttcctgc gccggctgcg cggcggcgag cctcgccggc cctctatctc
     1621 cgtttctccc gccatcaaca atgatcaccg ttccctgtaa tctgtgcggc cgggatgact
     1681 accgcgtacg cttcccggcc actacagagc cgagcgcgct gcgcgccgac gcctttcgct
     1741 gcacccacag cggctatggc caccatctgc aaatcgtcga gtgctgccat tgcggcctgg
     1801 tctacgccaa ccctcgctgg ccaaccgaca ccgtgttaga ggcatatacg tccgtcgagg
     1861 atgagaccta tgtccaggag cgccacggcc gcgagttgac ctttcgccgc cacctgcgcc
     1921 gtatcgagcg catcattggc ccggcccagg gtcggacact gttggacgtg ggggcctaca
     1981 tcggcgtctt cgtcgaggtg gcagcggcgg ctggctggcg ggcgcaaggc gtggagccgt
     2041 cggcgtgggc cgcggccgaa gcacagcgcc gcggtttggc cgtccacgat ggcacattgg
     2101 cgtcggtcga tctgccgccg gagtcgttcg acgtcatcac catgtgggac gtgatcgagc
     2161 acctgtccga cccggcggct gagctggccc gcgcgcggcg gctgctgcgg ccgggcggct
     2221 ggctggtggc ccatacgatg gacatcggcg cgccgctggc ccggatcatg ggcgcgcgct
     2281 ggccgtggct gatggacatg cacctctact acttcgaccg gcggacactg ccggccatgt
     2341 ttaccgccaa tggcctgcgg gtcgtgtggc aggggaccca gggccgctac ttgcggctgg
     2401 gctatctggc ctcgcgcgtg ggcggcctga acgcaggctt gggccggttg gcgggcgcgg
     2461 ttgtcaatgg gctggggctg ccggcggccg ctgtg
//
LOCUS       DAOSMQ010000483         2495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_41823_length_2495_cov_4.983197, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000483 DAOSMQ010000000
VERSION     DAOSMQ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41823_length_2495_cov_4.983197"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK829_13070"
     CDS             <1..483
                     /locus_tag="PK829_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOU42191.1"
                     /translation="AIGDIFVSATGDIHVLDERHFMLMKDGALLANSGHFDVEINKLA
                     LRDLSVDDPKLVRPFVEQFTMKHNGHRLNLLGQGRLINLAAAEGHPASVMDMSFAGQA
                     LAAKYLIDHKGELENRVYTIPAAVDQEIARIKLDAMGIYIDTLTAEQAHYLDSWEEGT
                     "
     gene            625..1251
                     /locus_tag="PK829_13075"
     CDS             625..1251
                     /locus_tag="PK829_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU42192.1"
                     /translation="MSIPKDTHKADRVGAVKLARVLSNVISPPTMFAGTGLALGVYER
                     GWWPGFAWGAFYAIVIALTPMLAIIYLLRTGRIGDLHMSATHERHIPYLTAAASGAAV
                     YALLTLLGGPVLLRCLAVFNVITLSALGLINSRWLISIHATAAAATWLIVTVVFGTVV
                     GLVALPVAVLICLVRLYLRRHTPAQIMAGAVLGLTVVAAMRVAGCFVV"
     gene            1266..2045
                     /locus_tag="PK829_13080"
     CDS             1266..2045
                     /locus_tag="PK829_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42193.1"
                     /translation="MFEQERFIGRLQRHVMTEARILTCFLSGSFGRRADDAYSDIDIA
                     LVYGSAADRDAAWAERREFVRAVMPYVAVRSFDAEHVRPFLHVALYSNGTKADYRFET
                     MDGLAPNPGDHEIRILKDGNRWAESYQAASSRLAPAQPYISPAALTELDDRFWVMFWD
                     VLRLIKRGDTDKPFPVYLQLLYFTLPPLLAALPREEPARAALLRAQYDGDAGATARGL
                     ADLLDAYIAARAAVIRRQNLVYPLNTAFEGEIRRLVTRLSR"
     gene            complement(2042..>2495)
                     /locus_tag="PK829_13085"
     CDS             complement(2042..>2495)
                     /locus_tag="PK829_13085"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HOU42194.1"
                     /translation="AAFGIGHLFYITAMLRLADRAGLVDSRRWIALAVWLLIGLVGWY
                     VVVYRGAVVGGKALSIVHWIALPYALLLATTAGLASGLALADSRFVLLAVGAALFLLS
                     DLILAGEMFSGLSFALIGDVIWLTYGPGQMLIVYSIGAALARLGAAAP"
BASE COUNT          407 a          853 c          827 g          408 t
ORIGIN      
        1 gccattggcg acattttcgt cagcgccacg ggcgatatcc atgtgctcga cgagcgccac
       61 ttcatgctga tgaaggacgg cgcgctgctg gccaactccg gccacttcga cgtggagatc
      121 aacaagctcg ccctgcgcga tctgtcggtt gacgacccca agctggtgcg gccgttcgtg
      181 gagcagttca ccatgaagca caatggccac cgtctgaacc tgctgggcca ggggcggctg
      241 atcaacctgg ccgcggccga gggacatccg gcgtcggtca tggacatgag cttcgccggg
      301 caggcgctgg cggccaagta tctgatcgac cacaagggcg agctggagaa ccgcgtctac
      361 accatcccgg ccgcggtcga tcaggagatc gcccgcatca agctggacgc gatgggcatc
      421 tatatcgaca cattgaccgc ggaacaggcc cactatctcg attcgtggga agagggcacc
      481 tgattctaat aggggccagg ttccaggggc caggttccag ggaaaagcct tcttccctgg
      541 cccctggccc ctagcccctt tccccttccg gggcgtggta gattgggaag tgggcggccc
      601 ggcctacagg gcggcccgac cgtcatgagc attccaaaag atacccacaa agccgaccgc
      661 gtcggcgcgg tgaagctggc gcgcgtcctg tccaacgtca tcagcccgcc gacgatgttc
      721 gccggcacgg ggctggctct gggcgtctat gagcgtggct ggtggccggg gtttgcctgg
      781 ggggcgttct acgccatcgt tatcgccctg acgccgatgc tggccatcat ctacctgttg
      841 cgcacggggc gcattggcga cctgcacatg agcgccaccc acgagcgcca tatcccctac
      901 ctgaccgcgg ccgcttccgg cgcggccgtc tacgccttgc tgaccctgct gggcgggccg
      961 gtgctgctcc gctgcctggc tgtgttcaac gtcatcaccc tcagcgcgct ggggctgatc
     1021 aatagccgct ggctgatcag catccacgcc acggccgcgg cggccacctg gctgatcgtc
     1081 actgtggtct ttggcacggt tgtggggctg gtggcgctgc cggtggccgt gctcatctgt
     1141 ctggtgcggc tatacctgcg gcggcacacg cccgcccaaa tcatggccgg ggcggtgctg
     1201 gggctgacgg tggtggcggc catgcgcgtc gccggctgtt tcgttgtcta aggctcccgc
     1261 aagctatgtt cgagcaagag cgtttcatcg gccgtttgca gcgccacgtg atgaccgagg
     1321 cgcgcattct gacctgcttc ctgtctggct cgttcggccg ccgcgccgac gacgcctatt
     1381 ccgacatcga catcgccctc gtctatggca gcgccgccga ccgcgacgcc gcctgggccg
     1441 aacggcgcga gttcgtccgc gcggtgatgc cctacgtggc cgtgcgctcc ttcgacgcgg
     1501 agcacgtgcg cccctttctg catgtggccc tctatagcaa cggaaccaag gccgactacc
     1561 gcttcgagac gatggacggg ctggccccca accccggcga ccacgagatc cgcatcctga
     1621 aggacggcaa ccgctgggcg gagagctacc aggcggcctc gtcgcggctg gctccggcgc
     1681 agccctacat cagcccggcg gcgctgaccg aactcgacga ccgcttctgg gtgatgttct
     1741 gggatgtgct gcggctgata aagcgcggcg atacggacaa gcccttcccc gtctacctgc
     1801 aactgcttta ctttactcta ccgccgctgc tggcggcgct gccgcgcgag gagccggcgc
     1861 gggcggcgtt gctgcgggcg caatacgatg gcgacgccgg cgcgacggcg cgcggcctgg
     1921 ccgacctgct ggacgcctac atcgccgcgc gggcggccgt catccgccgg cagaacctcg
     1981 tctacccgct caacaccgct ttcgagggcg aaatccggcg gttggtgacg cggctaagcc
     2041 gctagggcgc ggccgcgccc aggcgggcca gggccgcgcc gatggaatag acgattagca
     2101 tctggccagg gccataggtc agccagatca cgtcgccgat cagcgcgaag ctcaggccgc
     2161 tgaacatctc gccggcgagg atgaggtcgc tcaacaagaa cagcgccgcg cccactgcca
     2221 acaggacgaa gcgactgtcg gccagggcca ggcccgaggc cagcccggcc gtcgtcgcca
     2281 gtagcagcgc gtagggcagg gcgatccagt ggacgatcga cagcgccttg ccgccgacca
     2341 ccgcgccgcg atagacgacc acgtaccagc ccaccaaccc gatcagcagc cataccgcca
     2401 gggcgatcca gcggcgcgaa tcgaccagcc ccgcgcggtc ggccaggcgc agcatggcgg
     2461 tgatgtagaa gaggtggccg atgccgaagg cggcg
//
LOCUS       DAOSMQ010000484         2479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_42192_length_2479_cov_3.484323, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000484 DAOSMQ010000000
VERSION     DAOSMQ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42192_length_2479_cov_3.484323"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2224)
                     /locus_tag="PK829_13090"
     CDS             complement(<1..2224)
                     /locus_tag="PK829_13090"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU42195.1"
                     /translation="MLRFSDFAHLPQIDGLVRELVSEPRGLIVVAGLDSRPALEGPGD
                     SRPSFLPSGRATIFRVLVSELLDAHPGRRCVVVGEDRGVLHVARRFKSRLEFIAVKPP
                     LTYDQALRGLSGPRPGVVVIDRLTAENLPAILAVAHTGALVVSQVDTLYAGDDLVRYL
                     HDLGATAADLSSLRWVLSVQRLPTLCPICRQPAAVSADQMAQLEALGRRYPDLTAVHA
                     PGDGGAFYAPGRCATCQATGRQGDAAVFDFFHVPAGVEPAARHSRLPMGAYVWMLAQR
                     GQLALSDALDFDGDQLRRAHALLMATTRTASDLGAALERKTAELDTANRLLKQRMREL
                     VSLEGIGQALITWSDLSELGARVVRVAVELCKADRGALYYTRSADWAQVLAAHGWQGA
                     EIGLGLARALVYNGVSEREATPFLDPPPGITVAADAPALRAGLAVPLVAHGIPAGMML
                     IQSTRRAQFSQGEGALLQTLAGYAASAMQRAGLLEQLQSKVEALEAAQESIAQKERLE
                     RELELARQVQQSMLPRTFPSVPGLAFAAANAPARHVGGDFYDVIRLDADRVGLVIADV
                     SDKGMPAALYMALARSLILAESRQFDSPAVVLARVNQLLRELGEQDMFVTVFYGVLDP
                     AGRRLTYARAGHDRPLLLRDGAATALGGRGMALGLFDNTLFQVSEEELLLRAGDRLVL
                     YTDGLTDVVNPAGEMFDQRRLTALALRHAARPPADFCRALLADLAAYQGSAPQFDDMA
                     L"
     gene            complement(2271..>2479)
                     /locus_tag="PK829_13095"
     CDS             complement(2271..>2479)
                     /locus_tag="PK829_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42196.1"
                     /translation="VAVHHSEADDVVPVAWSHALCERLDALSAAGEIPYAPECTFYDL
                     QPHTFRGDGDRRFIEATIAFFDRH"
BASE COUNT          396 a          840 c          904 g          339 t
ORIGIN      
        1 ccagagccat gtcgtcgaac tgcggcgcgc tgccctggta ggcagccagg tcggccaaga
       61 gggcgcggca gaagtcggcc ggcggccgcg cggcgtggcg caacgccagc gccgtcagcc
      121 gccgctggtc gaacatctcg ccggccgggt tgaccacgtc ggtcagcccg tcggtgtaga
      181 gcaccagccg gtcgccggcc cgcagcagca gttcctcctc gctcacctgg aagagggtgt
      241 tgtcgaacaa ccccagggcc atgccccggc cgcccagcgc tgtcgcggca ccgtcgcgga
      301 gcagcaaggg ccggtcgtgc ccggcacgcg cgtaggtcag ccgtctcccc gccgggtcga
      361 ggacgccgta gaacaccgtc acgaacatgt cctgctcgcc cagttcccgc aagagctggt
      421 tgacgcgggc caggacgacg gccggcgagt cgaactggcg cgattcggcc aggatgaggc
      481 tgcgggccag ggccatatag agcgccgcgg gcatgccctt gtcggacacg tcggcgatga
      541 ccagcccaac gcggtcggcg tcgagacgga tgacatcgta gaagtcgccg ccgacgtggc
      601 gggcgggcgc gttggccgcg gcgaaagcca ggccagggac gctggggaag gtgcgcggca
      661 acatgctttg ctgcacctgc cgggccagtt ccagctcgcg ttccaaccgc tccttctggg
      721 ctatactctc ctgggcggct tccaacgcct cgactttgct ctgcaattgt tccagcagtc
      781 cggcccgctg catggccgag gcggcatagc cggccaacgt ttgcagcaag gctccctcgc
      841 cctgggagaa ttgcgcccgg cgggtggact ggatgagcat catcccggcg ggaatgccgt
      901 gggccaccag cggcacagcc agcccagccc gcagcgctgg cgcgtcggcg gccacggtga
      961 tgcccggcgg cgggtcgagg aagggcgtcg cctcgcgctc cgatacgcca ttgtagacga
     1021 gggcgcgggc caaacccagg ccgatctccg ccccctgcca gccgtgggcg gccagcacct
     1081 gcgcccagtc ggctgaacgg gtgtagtaga gcgcgccgcg gtcggcttta cataactcca
     1141 cggccacgcg caccacgcgc gcgcccagtt cgctcaggtc gctccaggtg atgagcgcct
     1201 ggccaatacc ctccagagag accagttcgc gcatgcgttg cttcagcagg cgattggcgg
     1261 tgtctagctc ggctgtcttg cgctccagcg ccgcgccgag gtcgctggcc gtccgcgtcg
     1321 tggccatcag cagggcgtgg gcgcggcgca gttggtcgcc gtcgaagtcg agggcatcgc
     1381 tgagggccag ttggccgcgc tgggccagca tccagacgta ggcacccatt ggcagccggc
     1441 tgtggcgcgc cgccggttcg acgccggccg gcacgtgaaa gaagtcgaac acagccgcgt
     1501 ccccctggcg gccggtcgcc tggcaggtag cgcagcggcc gggggcatag aacgcgccgc
     1561 catcgccggg cgcatggacg gcggtcaggt cgggataacg ccggccgagc gcctccagtt
     1621 gagccatctg gtcggcggag acggccgccg gctgccggca gatgggacag agcgtcggca
     1681 gccgctggac actgagcacc cagcgcagcg aggacaggtc ggccgcggtc gcgcccaggt
     1741 cgtgcaagta gcggacgagg tcgtcgccgg cgtagagcgt atccacctgg ctgacaacca
     1801 aagcgccggt gtgggccacg gccaggatag cgggcaaatt ctcggccgtc agccggtcga
     1861 tgaccacgac gccggggcgc gggccgctca gaccgcgcag ggcctggtcg taggtcagcg
     1921 gcggcttcac ggcgatgaac tccaggcgcg acttgaagcg ccgggcgacg tggagcacgc
     1981 cgcggtcttc gccgacgacg acgcaacggc gaccggggtg ggcgtcgagt agttcgctga
     2041 cgaggacgcg gaagatggtc gcccggccgc tgggcaagaa agagggccgg ctgtcgccgg
     2101 gaccttccag cgccggccgg ctgtccagcc cggcgacgac gatcagcccg cgcggctcgc
     2161 tgaccaattc ccgcaccagg ccgtcgatct gcggcaggtg ggcgaagtcg ctgaagcgca
     2221 gcatagggga agggtatctc gggttgggac cggtgtccag tgcctccgcc tcagtgccgg
     2281 tcgaaaaacg caatcgtcgc ctcaatgaac cgtcgatccc cgtcgccccg gaaggtatgc
     2341 ggctggaggt catagaaagt gcattccggc gcgtagggaa tctcgccggc ggcgctgagg
     2401 gcgtcgagcc gctcacagag ggcgtgcgac caggccacgg gcacgacatc atcggcctcg
     2461 ctatggtgga cggccacgg
//
LOCUS       DAOSMQ010000485         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_42265_length_2476_cov_5.075589, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000485 DAOSMQ010000000
VERSION     DAOSMQ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42265_length_2476_cov_5.075589"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PK829_13100"
     CDS             <1..1207
                     /locus_tag="PK829_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOU42197.1"
                     /translation="LLLVLLLVAGARGFIDWAVVRASFSTDPDVADATLARWETPGAN
                     WGAIIDNFRNLMVFRFPREETWRIWVTLAWLAALLIPTPYVYGRAAFRRSPVRRVLTW
                     LWLLTPVVSYLLLVGLSGNAVVPRLNPDIDWGGLLLTVIIAVVGIMASFPLGVLLALG
                     RRSRLSGLPAWLIWGAAAVLTVYFLISSTVPALGQTAGVAGKALALWPLLILLAAWLF
                     QRAFRGNVVALLSTIYIEVVRGVPLITILFMSIIIFPIFLPPGMEVLGTWRVLAAVTL
                     FAAAYLAENIRGGLQAIPKGQYEAADALGLSTPRKYRLIILPQALRAVIPAIVGQFIG
                     LFKDTTLVAIVALTELLGVANLISAQPDWLGVHREPYVFIALIYFIGNFFMASYSRRL
                     ERRLGVGER"
     gene            1240..2004
                     /locus_tag="PK829_13105"
     CDS             1240..2004
                     /locus_tag="PK829_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOU42198.1"
                     /translation="MTTEHKTPSTEEIIICRDVHKWYGDFEALRGISLTVRRGEVIVI
                     IGPSGSGKSTFIRTINRLEEHQQGDIIVDGITLTHDIRNIAEVRREVGMVFQQFNLFP
                     HLTVLENIMLAPVNVRKWTREQAEERARHWLQRVGIPEQADKYPGQLSGGQQQRVAIA
                     RTLAMEPLILMFDEPTSALDPEMIKEVLDVMRDLATSGYTILAVTHEMGFARTVADRV
                     IFMDGGLIVEENTPEDFFANPTNERTKQFLSQILSH"
     gene            complement(2084..>2476)
                     /locus_tag="PK829_13110"
     CDS             complement(2084..>2476)
                     /locus_tag="PK829_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU42199.1"
                     /translation="TQMNSQTVSSAANFTMHAAVAALKGPKDTTRAMRDEYQQRRDFM
                     VPALNAIDGVECANIEGAFYLFPRFPNSTKTSAELAEALIDKAGIASVPGGAFGRSGE
                     GHVRFTIATAMRDLEKAVDKLAKVAREL"
BASE COUNT          372 a          829 c          812 g          463 t
ORIGIN      
        1 gctgctgctg gtgctgctgc tggtggccgg ggcgcgcggc ttcatcgact gggccgttgt
       61 ccgggccagc ttctccactg acccggacgt ggccgacgcc acgctggcgc gttgggagac
      121 gccgggcgcg aactggggcg caatcatcga caacttccgc aacctgatgg tcttccgctt
      181 cccgcgcgag gagacatggc gcatctgggt gacgctggcc tggctggcgg ctctgctcat
      241 ccccacgccc tacgtctatg gccgggccgc gtttcggcgc tcgccggtgc gacgcgtgct
      301 gacgtggttg tggctgctga cgccggtggt cagttatctg ctgctcgtcg gcctgtcggg
      361 caacgctgtc gtgccacggc tgaatcccga catcgactgg ggtggcctgc tgctgacggt
      421 catcatcgcc gtggtgggca tcatggcttc gttcccgctg ggggtgctgt tggcgctcgg
      481 ccgccgcagc cgtctcagcg gtctccccgc gtggctcatc tggggggccg cggccgtgct
      541 gacggtctac tttctgatca gcagcaccgt ccccgcgctg ggccagacgg ccggcgtcgc
      601 cggcaaggcg ctggcgctct ggccgctgct catcctgctg gcggcctggc tgttccagcg
      661 cgccttccgc ggcaacgtcg tcgccctgtt gagcacgatc tacattgagg tcgtccgcgg
      721 cgtgccgctg atcaccatcc tgttcatgtc gatcatcatc ttccccatct tcctgccgcc
      781 ggggatggag gtgctgggca cgtggcgcgt gctggccgcg gtgacgctct tcgccgccgc
      841 ctacctggcc gagaacatcc gcggcggctt gcaggccatc cccaaggggc aatacgaggc
      901 cgccgacgcc ctggggctga gcacgccgcg caagtaccgg ctcatcatcc tgccccaggc
      961 gttgcgcgcc gtcattccgg ccatcgtcgg ccagttcatc ggcctgttca aggacacgac
     1021 gctggtggcc atcgtcgccc tgaccgagct gttgggcgtc gccaacctca tctcggcgca
     1081 gcccgattgg ctgggcgtcc accgcgagcc atacgtgttc atcgccctca tctacttcat
     1141 cggcaacttc ttcatggcat cctatagccg gcggctggag cggcgtttgg gcgtcggcga
     1201 gcgttgaggc gccggcgcgg cccatcagga gacacaggca tgacgacgga acacaaaacc
     1261 ccgtccacgg aagagatcat catctgccgc gacgtgcaca agtggtatgg cgatttcgag
     1321 gcgctgcgcg gcatcagcct gacggtcagg cgcggcgagg tcatcgtcat catcggcccg
     1381 tccggctccg gcaaatcgac tttcatccgc accatcaacc ggctggagga gcaccagcag
     1441 ggggacatca tcgttgatgg catcaccctg acccacgaca tccgcaacat cgctgaggtt
     1501 cggcgcgagg tgggcatggt cttccagcag ttcaacctct tcccgcacct gaccgtgctg
     1561 gagaacatca tgctggcccc ggtgaacgtg cgcaagtgga cgcgcgaaca ggctgaggag
     1621 cgcgcgcgtc actggctaca gcgcgtcggc atccccgaac aggccgacaa gtaccccggc
     1681 cagctctccg gcggccagca gcagcgcgtg gccattgccc ggacgctggc catggagccg
     1741 cttatcctga tgttcgacga gccgacctcg gccctggacc cggagatgat caaggaagtg
     1801 ctcgacgtga tgcgcgacct ggccacgtcg ggctacacca ttctggccgt aacccatgag
     1861 atgggcttcg cccgcaccgt ggccgaccgg gttattttca tggacggcgg cctgatcgtc
     1921 gaggagaaca cgcctgagga tttcttcgcc aaccccacga acgagcggac gaagcagttc
     1981 ctgtcccaaa tcctgagcca ctgaagagag gggacaggtt ccaggggcca ggttccaggg
     2041 aaagagtgct cctcccctgg aacctggccc ctggaacctt cttctatagc tcgcgcgcga
     2101 ctttagccag cttatcaacg gccttttcca ggtcgcgcat ggctgtggcg atggtgaagc
     2161 ggacgtggcc ttcgccactg cggccgaaag cgccaccggg cacggaggcg atgccggcct
     2221 tgtcgatgag cgcctcggcc agctcggcgc tggtcttcgt gctgttgggg aagcgcggga
     2281 acaggtagaa cgcgccctcg atgttggcgc actcgacgcc gtcaatggcg ttcagcgccg
     2341 gcaccatgaa gtcgcgccgc tgttggtatt cgtcgcgcat ggcccgcgtc gtgtccttcg
     2401 gccccttcag cgcggccacg gcggcgtgca tggtgaagtt ggccgccgag gagacggtct
     2461 ggctgttcat ctgcgt
//
LOCUS       DAOSMQ010000486         2473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_42355_length_2473_cov_3.343672, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000486 DAOSMQ010000000
VERSION     DAOSMQ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42355_length_2473_cov_3.343672"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PK829_13115"
     CDS             <1..426
                     /locus_tag="PK829_13115"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOU42200.1"
                     /translation="QPNFSTCFGAPFMPLRPGVYAKLLGEKIEKHGAQVWLVNTGWTG
                     GPYGVGSRMKLAYTRRMIHAALDGELDNVPMVEEPYFGLAVPESVPDVPSDILMARNT
                     WADRDAYDAQAQKLVGMFKENFKQFEELVSDDIKGAGPH"
     gene            627..1268
                     /locus_tag="PK829_13120"
     CDS             627..1268
                     /locus_tag="PK829_13120"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOU42201.1"
                     /translation="MYELPLFPLNLVLFPGMPLPLHIFEERYKEMVADCLRENRPFGV
                     VLIAEGRAEGGAPARPHVVGCTAEITQVQPLDDGRMVIMTVGRERFRIARLRHDRPYL
                     VGLVEPAPLLAEDATSVAAGAARLEPLVMAYLKKLVSMGNIDTMPDDPPDDPASLAYL
                     GAALIQLPPEEKQDLLAFDRLTELEAALRRAYHDELALLRTVPAQDIGLFSVN"
     gene            1454..2083
                     /locus_tag="PK829_13125"
     CDS             1454..2083
                     /locus_tag="PK829_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HOU42202.1"
                     /translation="MKIAIIGTGRVAAALGKGWAARGHMVTFASRQPGSEKVATLVAA
                     AGPNAAAARVADAVARSSTVVLAVPFSAAQETLAMAGDLAGKLLIDCTNPIAPGLRLL
                     YNGDRSGAEQIAAWAPGARVIKAFNTTGAENMAAPAYPGGAASMFLCGDDEMGKAAVW
                     QLAEEIGFEPVDAGALSAARHLENLALLWIHLAQVNGLGRGIAFRLLRR"
     gene            complement(2155..>2473)
                     /locus_tag="PK829_13130"
     CDS             complement(2155..>2473)
                     /locus_tag="PK829_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42203.1"
                     /translation="TSATLLSAVLADHVGRRLKGALHPDLADALALTRSRLDRVAAAL
                     RAVPDIPAQITAGEFGPLHGWLRENIYRHGSKFTANELIARVCGGPLSIDPYVAYLRD
                     KFA"
BASE COUNT          400 a          809 c          878 g          386 t
ORIGIN      
        1 cagccaaact tcagcacctg cttcggcgcg ccgttcatgc ccctgcggcc gggcgtctat
       61 gccaagctgc tgggcgagaa gatcgagaag cacggcgcgc aggtctggct ggtcaacacc
      121 ggctggacag gcggccccta cggcgtgggc agccgcatga agctggccta cacccggcgg
      181 atgatccacg ccgccctcga cggcgagctg gacaacgtgc ccatggtcga agagccgtac
      241 ttcgggctgg ctgtgccgga gagcgtgccc gacgtgccgt cggacatcct gatggcccgc
      301 aacacctggg ccgacaggga cgcctacgac gcccaggccc agaagctggt gggcatgttc
      361 aaggagaact tcaagcagtt cgaggaactg gtcagcgacg acatcaaggg cgccggcccc
      421 cactgagccg aacacaccct ccattagggg cagaaacccg ggccaacgcc cgggtttttg
      481 tttgccagtg gtgcgtcgca ctttcctgag tgcgtcgcac cttcttcggc ggcccgaggg
      541 gagcgaagaa agtgccaggc acggagaagt tcttcgccgg gcaacgaatc ggctatactg
      601 tgcgtatagg cgacaaggac tcgactatgt atgaactgcc gctctttccg ctgaacctcg
      661 tcctctttcc ggggatgccg ctgccgttgc acatctttga ggagcgctat aaggagatgg
      721 tggccgactg tctgcgcgag aaccggccgt ttggcgtcgt cctgatcgcc gaggggcggg
      781 cggaaggcgg cgcgccggcg cgcccccacg ttgtcggctg cacggccgag atcacccagg
      841 tgcagccgtt ggacgacggc cgcatggtca ttatgaccgt cggccgcgag cgcttccgca
      901 tcgcccggct gcgccacgac cgcccctacc tggtcggcct ggtcgaaccg gccccgctgc
      961 tggcggagga cgcgacctcc gtggccgccg gcgcggcccg gctggagccg ctggtcatgg
     1021 cgtacctgaa gaagctggtc agcatgggca acatcgacac gatgcccgac gacccgcccg
     1081 acgatcccgc ctcgctggcc tatctgggcg cggcgctcat ccagctgcca ccggaggaga
     1141 agcaagacct gctggcgttc gaccggctga cggagctgga ggcggcgctg cggcgggcct
     1201 accacgacga gctggccctg ctgcgcaccg tccccgcgca agacattggg ctgttttctg
     1261 taaactgacc gccgacccaa tgccaggggc caggttccag gggccagggg ccagggcaag
     1321 agcgtgccag cttactgacc gactgaccca ctgaccgact ggcccactgc ttactgatct
     1381 actggcccat gctatctttg cccctcgccg ccgaatcgtg tatcttgggc gcgtctaata
     1441 aggaagggtc ggcatgaaga ttgcgatcat tggcaccgga cgggtggcgg cggcgctggg
     1501 caagggctgg gcggcccgcg gccacatggt cacctttgcc tcgcgccagc cgggtagcga
     1561 gaaagtggcc acgctggtgg cggcggccgg gccaaacgcg gccgccgcgc gcgtggccga
     1621 cgccgtggcc cgcagcagca ccgtcgttct ggccgtgccc ttctcggccg cccaggagac
     1681 gctggcgatg gccggcgacc tggccggcaa gctgctgatc gactgcacca accccatcgc
     1741 ccccggcctg cggctgctct acaatggcga tcgttccggc gcggaacaga tcgccgcctg
     1801 ggcgccgggc gcgcgggtga tcaaggcgtt caacacgacc ggcgcggaga acatggccgc
     1861 gccggcctac cctggcggcg cggcgtcgat gttcctctgt ggcgatgatg agatgggcaa
     1921 ggcggccgtc tggcagctgg ccgaagagat cggcttcgag ccggtggatg ccggggcgct
     1981 gtcggccgcg cgccatctgg agaatctggc cctgttgtgg atccacctgg cgcaggtgaa
     2041 cgggctgggc cggggcatcg ccttccggct gctgcgacgc tagggatagg gattaggggc
     2101 tagggattag ggattaggga agagcgctct tccctagccc ctagccccta tcccctaggc
     2161 aaacttgtcg cgcaggtagg ccacgtaggg gtcgatggac agcgggccgc cgcagacgcg
     2221 ggcgatcagc tcgttggcgg tgaacttgga gccgtggcga tagatatttt cgcgtagcca
     2281 gccgtggagc gggccgaact cgccggcggt aatctgggcg ggaatgtcgg gcacggcgcg
     2341 cagggccgcc gccacccggt cgaggcgtga ccgggtgagg gccagggcgt ccgccaggtc
     2401 ggggtgcagg gcgcccttga gccggcggcc cacgtggtcg gcgagcacgg ccgagagcag
     2461 cgtggccgac gtc
//
LOCUS       DAOSMQ010000487         2470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_42416_length_2470_cov_3.491511, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000487 DAOSMQ010000000
VERSION     DAOSMQ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42416_length_2470_cov_3.491511"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PK829_13135"
     CDS             <1..1222
                     /locus_tag="PK829_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42204.1"
                     /translation="LGAFDLRAPEAADWRSGKLAAGVRAIQAVPPSGWRVTGIACAGD
                     SDAGSVFDPIAGTALIDLDGAETLGCVFQLARTAPGARLTIRNEAAPADDADFAYTGD
                     LGPFLLHAPSAAQTSYSLMPGVYRVHELLHPLWTLGDIACSGDADGGSVASREDASVI
                     VDLDLGEEITCVFRHVRAAAGTGTITLAQAASPAEAASFAYDGTLGAFVLTTPASTTR
                     TWPSLPPGSYTIQQTPPAGWQLDAITCAGDSDAGSSLLPGERMAVLDLDAGENLVCTF
                     ANSRPVGSGSIAIVHAPTPADETAFRFRGALGGFTLRAPSRPVRTFTGLAAGAYSVNV
                     RLPEGWQLGAITCEGDSDRGTVIDTATAAATIDLDLGEAITCRFASVPEDAPPPPPPP
                     PPAASRVYIPYLGR"
     gene            complement(1307..2059)
                     /locus_tag="PK829_13140"
     CDS             complement(1307..2059)
                     /locus_tag="PK829_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOU42205.1"
                     /translation="MHHSLATRKLNVWYGQQHALKNINIDLPIRQITAIIGPSGCGKS
                     TFLKSLNRLLELNEIVRIEGEVLLDGENIYAPGSDVTDVRTRIGMLAQKPFPLPMSIY
                     DNVAYGMKIHGMHNGHVADNVERALRAVGLWSEVADRLKAPATGLSGGQQQRLCLARA
                     IAVEPEILLCDESTASLDPIAAQGIEALLMELRGDYTLVIVTHNIAQARRLADYVLFM
                     WLGELVEHGPAAEFFANPQQKLTQDYLARRIG"
     gene            complement(2107..>2470)
                     /locus_tag="PK829_13145"
     CDS             complement(2107..>2470)
                     /locus_tag="PK829_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU42206.1"
                     /translation="VPKELRESTLALGTTRFEMLGMLLKQTLPGLFTAVLLAFGRAIG
                     DGAAPMFTAGYTDRLPQGLMQPVATLPLAIFFQLSAPFKEVQTRAYASALILTAVILI
                     ISFLAQYTSRRLGRNVIR"
BASE COUNT          415 a          839 c          805 g          411 t
ORIGIN      
        1 cctgggtgcg ttcgacttgc gcgctccgga ggcggccgac tggcgcagtg gcaagctggc
       61 cgcgggcgtt cgcgccatcc aggctgttcc gccctccggt tggcgcgtga ccggcatcgc
      121 ttgcgccggc gatagcgatg cgggcagcgt tttcgacccg atcgcgggca cggcactcat
      181 cgatctggat ggcgcggaga cgctaggctg cgtcttccaa ctggcgcgta ccgcgccagg
      241 agcgcgcctg accattcgca acgaggccgc gccggccgat gacgccgact tcgcctatac
      301 gggcgacctc ggcccattcc tattacatgc cccctcggcg gcgcagacga gctatagcct
      361 gatgcccggc gtctaccgcg tgcacgaact tctgcaccct ctatggacgc tgggcgacat
      421 cgcctgtagc ggcgacgccg acggtggctc ggtcgccagc cgcgaggacg cctcagtgat
      481 cgtcgatctc gacctgggcg aagagatcac ctgcgtcttt cgccacgtgc gcgcagcggc
      541 cggcacaggc acgatcaccc tcgcccaggc agccagtcca gcggaagcag cctccttcgc
      601 ctacgacggc acgctggggg cgttcgtcct gaccacaccc gccagcacca cgcgcacgtg
      661 gccgtcgctg ccgccgggga gctacacgat ccagcagacg ccgccggccg gctggcagct
      721 ggacgccatc acctgtgccg gcgacagcga cgccggcagc agcttgctgc ccggcgaacg
      781 catggccgtg ctcgacctcg atgccgggga gaacctcgtc tgcacttttg ccaattcgcg
      841 cccggtcggc agcggtagta ttgctatcgt ccatgcgccc acgccggccg acgagaccgc
      901 tttccgtttc cggggcgcgc tgggcggctt caccttgcgc gcgccgtcgc ggccggtgcg
      961 aacctttacc gggctggccg cgggcgctta cagcgtcaat gtgcgcctac ccgaaggctg
     1021 gcagctcggc gcgatcactt gcgagggcga cagcgatagg ggcacggtca tcgacacggc
     1081 gacggccgcc gcgaccatcg atctggacct gggcgaggcc atcacctgtc gcttcgccag
     1141 cgtgccagag gacgcgcctc cgcctccgcc gccgccaccc ccggccgcca gtcgggtcta
     1201 catcccctac ctcggccgct agtccccacc cacacaaaca aacccggcca cggcgggtgc
     1261 cgcggccggg taaccaccac tccctagaag ccggaagcgg ggagcactaa ccaattcgac
     1321 gcgccaggta gtcctgggtc agcttttgct gggggttggc gaagaactcc gccgcggggc
     1381 cgtgctccac cagctcaccc agccacataa acagcacgta gtcggccagg cggcgcgcct
     1441 gggcaatgtt gtgggtgaca atcaccagcg tgtaatcgcc gcgcagttcc atgagcagcg
     1501 cttcgatgcc ctgtgcggcg atagggtcga gtgaagcagt ggactcgtcg cacagcagaa
     1561 tctctggctc gacggcgatg gcgcgagcca ggcaaaggcg ctgctgctgc ccgccggaca
     1621 ggccagtggc gggagccttg aggcggtcgg cgacttcgct ccacagcccc acagccctga
     1681 gtgccctctc gacgttgtcg gccacgtggc cattgtgcat gccgtggatc ttcatgccgt
     1741 aggcaacgtt gtcgtagatc gacatgggca acgggaacgg cttttgggcc agcataccga
     1801 tgcgcgtgcg cacgtcggtt acatcgctgc cgggggcgta gatgttctcg ccgtccagca
     1861 gcacttcgcc ctcaatgcgc acgatctcgt ttagctccag tagccggtta aggctcttca
     1921 gaaaggtgga cttgccacag cccgacggcc caatgatggc cgtgatctgg cggatgggca
     1981 ggtcgatatt gatattcttg agtgcgtgtt gctgtccata ccacacgttt agcttgcgcg
     2041 tggccagtga gtggtgcatg gttgtctccc catcgcaacg aagcggcggc gcttcgtcaa
     2101 cggcggttag cgaataacat tacgacccag ccgccgcgag gtgtactggg ccagaaagct
     2161 aatgatcagg atgacggccg tcaggatcag cgccgacgcg taggctcgcg tctgcacttc
     2221 cttgaacggc gcgctcagct ggaagaagat cgccagcggc agggtggcga cgggttgcat
     2281 gaggccctgc gggaggcggt cggtgtaacc ggcggtgaac atgggcgcgg ccccgtcgcc
     2341 aatagcccgg ccaaaggcca acagcacggc cgtaaacaga ccgggcaacg tctgcttaag
     2401 caacatgccc agcatctcga agcgggttgt gcccagcgcc agggtcgatt cgcgcaactc
     2461 tttgggcacc
//
LOCUS       DAOSMQ010000488         2467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_42487_length_2467_cov_4.852405, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000488 DAOSMQ010000000
VERSION     DAOSMQ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42487_length_2467_cov_4.852405"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PK829_13150"
     CDS             <1..330
                     /locus_tag="PK829_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42207.1"
                     /translation="VVLLLAAGLGAVAGAVLSFAQFLALRGHVAHAGRWIPANMLAWA
                     VGMPVVFWAMDLAFKLSSTAAAIAVVALALLAMGAIVGAIHGVVLVGMVSGSEEGSHP
                     RSLAAGR"
     assembly_gap    439..448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <449..878
                     /locus_tag="PK829_13155"
     CDS             <449..878
                     /locus_tag="PK829_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42208.1"
                     /translation="SPSRRNVVLIGLVILVALSLLFAAGRLLRVGDGLSSDVVATPPL
                     QLTPDPGEEVDPQQPETDVPTDSLRELSLAVAAVITALTGLLGLVATQIWRGREEGRL
                     DQKHALALERERLELERERLALEKERLEVERQRQAMRGSG"
     gene            916..1374
                     /locus_tag="PK829_13160"
     CDS             916..1374
                     /locus_tag="PK829_13160"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOU42209.1"
                     /translation="MSNIHVSVSRVIDAPADVIYGILSDYRAGHPSILPKPYFKELVV
                     EEGGVGEGTVARVGMEVMGSQQTLRLRVTEPEPGRVLREEDAAAGVVTLFTVEPLAAG
                     RSRVTFDTDMPASDGLRGWLERRTTPFFMRRIYHAEQSLLADMARTRAGQ"
     gene            1371..2060
                     /locus_tag="PK829_13165"
                     /pseudo
     CDS             1371..2060
                     /locus_tag="PK829_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
     gene            2151..>2467
                     /locus_tag="PK829_13170"
     CDS             2151..>2467
                     /locus_tag="PK829_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU42210.1"
                     /translation="MEDVIIIGAGLAGLATAIDLQKAGRGVTILERYKIAGGRCADWV
                     MDGMRVESGLHRWLGFYKALPELFRKADLDPDKAVIWEDELEIRLPDGGPQAVLGLSP
                     LHRP"
BASE COUNT          384 a          773 c          872 g          428 t
ORIGIN      
        1 gtggtgctgc tgctggccgc ggggctgggg gcggtggccg gggcggtgct gtcgttcgcg
       61 cagttcctgg ctctgcgcgg ccacgtcgcc cacgccgggc gctggattcc ggctaacatg
      121 ctggcctggg ccgtgggcat gcccgtcgtc ttctgggcca tggatctcgc cttcaagctg
      181 tcgagcacgg ccgcggccat cgccgtcgtc gccctggccc tgctggccat gggggccatc
      241 gttggggcca tccacggcgt ggtgttggtc ggcatggtgt cggggagtga ggaaggcagc
      301 catcctcgat cgctggccgc cggccgctga ccgccggcca ctggcccagg ttgatccgca
      361 gccgcaagcg gctatactgc cggcacggaa cggaaccact gctgtgtgtc gaacgtcttt
      421 caggtagcta tggccgatnn nnnnnnnnat cgccttctcg ccgcaatgtg gtgctcatcg
      481 ggctggtcat cctggtggct ctcagtctgc tgttcgccgc cggccgcctg ttgcgcgtcg
      541 gcgacggcct cagcagcgac gtcgtggcca cgccgccgct gcaactgacc cccgaccccg
      601 gcgaggaggt cgatccgcag cagccggaaa ccgacgtccc gaccgatagc ctgcgcgagt
      661 tgtcgctggc cgtggccgcg gtcatcacgg ccctcaccgg tctgctgggc ctggtggcga
      721 cgcagatctg gcgcggccgc gaggaggggc ggctcgacca gaagcacgcc ctggccctgg
      781 agcgggagcg gctggaattg gagcgggagc gcctggcgct ggagaaggag cgcctggaag
      841 tggagcggca gcggcaggcc atgcgcggtt ctggatagcg ctattgccgc gccgtgctgc
      901 tggaaggagt aggccatgag caatatccat gtatccgtgt cgcgcgtcat cgacgcgccg
      961 gccgatgtca tttacggcat cttgtctgac taccgcgccg gtcacccctc cattttgccc
     1021 aagccatact tcaaggagct ggtcgtcgaa gaaggtggcg ttggcgaggg cacggttgcc
     1081 cgcgtaggca tggaggtgat gggtagccag cagaccctgc gcctgcgggt gacggagcct
     1141 gaaccggggc gcgtgttgag ggaggaggat gccgccgcgg gggtcgtgac gctctttacg
     1201 gtcgagccgc tggccgccgg ccgcagccgg gttacatttg acaccgacat gcccgcgtcc
     1261 gatggcctgc gcggctggct ggaacggcgc acgacgccct ttttcatgcg acgcatctat
     1321 cacgccgagc agtcgctgct ggcggacatg gcgcggacgc gggccgggca atgagcgccg
     1381 cccgcgtcga cgcggggtcg ccctacgcgg aggcggccga agccattggc ctgcgctacg
     1441 tcagcgacga cgagccgggc atccgccgcg agcgcgttgg cgacgacttc cagtaccgcg
     1501 acgaacgcgg tgagcggatc gacagtccgc gcctgctggc acgcatcgag gccctgcgca
     1561 ttccgccggc ctgggaagag gtctggattt gccgccgggc caacggccac ttgcaggcca
     1621 ccggccgcga cgccagaggg cgcaaacagt accgctatca ccccgactgg agcgccacgc
     1681 gcaaccagac caagtacgac cgtatggccg agtttggcgc ggcgctgccc cacctgcgcg
     1741 agcgcatcga cgccgaccta tcacggcgcg gcctgccccg cgagaaggta gtggccaccg
     1801 tgctgcggct gatggagatg acttttatcc gcgttggcaa cgtcgagtac gcgcgggcca
     1861 atcgctccta tggcctgacg accctgcgcg ataagcatgt gcgtgtcgag ggggccacca
     1921 tccacttcca gtttatcggc aagagcggcc agaaacatgc catcgacgtg cgcgaccgca
     1981 agctggcgcg catcgtcaag cagtgccgcg acctgcccgg ctatgagctg tttcagtaca
     2041 ttgacgaagc gggcgatagg aattcctaat tcgctttggt cgggtttttc cccgtatcgc
     2101 actgcgtcaa tgttccgtat actgagagga tagcaaggag caaagcaacg atggaagatg
     2161 tgatcattat tggcgcgggg ttggcggggt tagccacggc cattgactta cagaaggcgg
     2221 gacgcggcgt gacgattttg gagcggtaca agatcgccgg tgggcgctgt gctgattggg
     2281 tgatggatgg catgcgcgtc gagtcgggcc tgcaccgctg gctgggcttc tacaaggcgc
     2341 tgcccgagct tttcaggaaa gctgacctgg accctgataa ggccgtcatc tgggaggacg
     2401 agttggagat tcgcctaccc gatggcgggc cgcaggccgt gctgggcctg tcgccgctgc
     2461 accggcc
//
LOCUS       DAOSMQ010000489         2465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_42532_length_2465_cov_5.370954, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000489 DAOSMQ010000000
VERSION     DAOSMQ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42532_length_2465_cov_5.370954"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK829_13175"
     CDS             <1..332
                     /locus_tag="PK829_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOU42211.1"
                     /translation="VLLVPLPLLDELDRVVTQKPRLARYIQPEQLAALRGILLDIGEV
                     VPPISGVLPIVSRDRKDDYLLAYAVVGEADYVVTGDEDLLVLKRLDKLQILSPPAFAA
                     LLRDKES"
     gene            complement(386..1126)
                     /locus_tag="PK829_13180"
     CDS             complement(386..1126)
                     /locus_tag="PK829_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOU42212.1"
                     /translation="MTRMALISDIHGNLTALEAALADIERHGAERIVCLGDVAQEGPQ
                     PVAVIHRLHDLAIPVVMGNTDDWLLNPKPKKVKSEKDRQELEIDRWVLAQLTDTERAL
                     MQDYAQTLTFDLDGASLLCYHGSPISCEHRIRPTTPAKRLKELFAGHNETLFAGGHTH
                     EPMLRRFGPSLILNPGSVGAPVVERQTSTEDDYTPSWAEYALVEAKGADLRVTLRRVP
                     YALADFAAAVRASGMPHAGYLLKGWRQV"
     gene            1230..2141
                     /locus_tag="PK829_13185"
     CDS             1230..2141
                     /locus_tag="PK829_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU42213.1"
                     /translation="MTVSRIQMTDAGPMFSRLVAGVWRLGDWGMDTGALLGFIHGCLD
                     LGITTFDNADIYGGYTCEERFGAALAAEPGLRDRLELVTKCGIQLVSGNRPANRVHHY
                     DTSRAAIVRAAENALRLFHTDRLDLLLIHRPDPLLDADEVAEAFAALRVAGKARAFGV
                     SNFMPWQFDLLQARLDFPLATNQIELSVLALDPLHDGTLDQAQRLRVPPMAWSPLAGG
                     RLFDPGDERAARVRRALAGVGQELGGAGVDQVALAWLLRHPARVLPVLGTGRLERVQA
                     AAMAEALTLDRQQWFAVWEASAGHEVP"
     gene            complement(2138..>2465)
                     /locus_tag="PK829_13190"
     CDS             complement(2138..>2465)
                     /locus_tag="PK829_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42214.1"
                     /translation="TPAPPLFCLPLVWRADGQPEAAYRVFVHLVDAAGHIIAQSDAAP
                     ANWTRPTTGWLRGEVILDTHTLTLPEPLPAGPLTLRIGLYDPDTGARLASGGADFVTI
                     PWPLAP"
BASE COUNT          369 a          800 c          868 g          428 t
ORIGIN      
        1 ttgtcctgct cgtaccgctt cctttgttag acgagctaga ccgtgttgtg acccaaaagc
       61 cacgtctggc caggtacatc cagcctgagc aattggctgc tctgcgcggt atcctgctgg
      121 acattggtga agttgtgccc cccatcagcg gcgtccttcc tattgtttcg cgagatcgta
      181 aggacgacta tctattagcc tatgcagttg tcggcgaggc ggattacgtg gtgactggcg
      241 atgaagatct actcgttctc aagcggctgg acaagctgca aatcctctcg ccgccggcat
      301 ttgcagccct tcttcgcgac aaagaatcgt gacgcctcct atggcctcca gtagccgcgc
      361 ggcgggccgc gccgccgggc aggggctaga cctgccgcca gcccttcagc agatagccgg
      421 cgtggggcat gccactggcg cggacggccg cggcgaagtc ggccagcgcg tagggaacgc
      481 gccgcagcgt tacgcgcaaa tcggcccctt tcgcctcgac gagggcatac tcggcccagg
      541 agggggtgta gtcgtcttca gtgctggtct ggcgttcgac cacgggcgcg ccgacgctgc
      601 cggggttgag gatcaggctg gggccgaagc ggcgcagcat tggctcgtgg gtgtggccgc
      661 cggcgaagag cgtctcgttg tggccggcga acaactcctt cagccgtttg gccggcgtgg
      721 tggggcggat gcggtgttcg cagctgatgg gcgagccgtg gtagcagagc agggacgcgc
      781 cgtccaggtc gaacgtcagt gtttgcgcat agtcctgcat cagcgcccgc tctgtgtccg
      841 tcaactgcgc cagcacccag cggtcgattt ctaactcctg gcggtctttc tctgacttga
      901 ccttcttcgg cttgggattc agcagccagt cgtcggtgtt gcccatgacg accgggatgg
      961 ccaggtcatg caggcgatgg atgacggcca cgggctgtgg cccttcctgg gccacgtcgc
     1021 ccaggcagac aatgcgttcc gcgccgtggc gctcaatgtc ggctagcgcc gcctccagcg
     1081 cggtcaggtt gccgtggata tcggaaatca aagccattcg cgtcatgggc catacctccg
     1141 gcgggaatga taaccgccgg gccgcggagt tgtcgaatcc gcgtccgcgt tcataatcac
     1201 ttcctattca cgcttaagga gaaatgggca tgaccgtctc gcgcattcaa atgaccgacg
     1261 ccgggccgat gttctcgcgg ctggtggccg gggtgtggcg gctgggcgac tggggtatgg
     1321 acaccggcgc gctgctgggc ttcatccacg gctgcctcga cctgggcatc accaccttcg
     1381 acaacgccga catctacggc ggctacacct gcgaggagcg cttcggcgcg gcgctggcgg
     1441 ccgagccggg cctgcgcgac cgcctggaac tggtcaccaa gtgcggcatc cagcttgtca
     1501 gcggcaaccg gccggccaac cgcgtccacc attacgacac cagccgcgcc gccatcgtcc
     1561 gcgcggccga gaacgcgctg cggctattcc acaccgaccg cctcgacctg ttgctcatcc
     1621 atcgccccga cccgctgctg gacgccgacg aggtggccga agcgttcgcg gccctgcggg
     1681 tggctggcaa ggcgcgggcc tttggcgtca gcaacttcat gccctggcag ttcgacctgt
     1741 tgcaagcgcg cctcgacttc ccgctggcca ccaaccagat cgagctgtcg gtgctggccc
     1801 ttgacccgct ccacgacggc acgctcgacc aggcccagcg gttgcgcgtg ccgccgatgg
     1861 cctggtcgcc gctggccggc ggacgactgt tcgaccctgg cgacgagcgg gccgcgcggg
     1921 tgcggcgggc gctggcgggc gtcggccagg agttgggtgg cgcgggcgtc gatcaggtcg
     1981 cgctggcctg gctgctgcgc catccggcgc gggtgttgcc ggtgctgggc acgggccggc
     2041 tggagcgcgt gcaggccgcg gcgatggccg aggcgctgac gctcgaccgg caacagtggt
     2101 tcgctgtctg ggaggcgtcg gccgggcacg aagtgcccta gggcgcaagc ggccagggga
     2161 tcgtcacgaa atcggccccg ccactggcca gccgcgcacc cgtgtcgggg tcatacaggc
     2221 cgatccgcag cgtcagcggc ccggccggca acggctccgg cagcgtcagc gtatgcgtgt
     2281 cgaggatgac ctcgccccgc aaccagccgg tcgtcggccg cgtccagttg gccggggcgg
     2341 catcggactg ggcgatgatg tggccggcgg catcgaccag gtggacgaag acgcgatagg
     2401 cggcctctgg ctggccgtcg gcgcgccaga cgagaggaag gcagaagagt gggggagcgg
     2461 gggtg
//
LOCUS       DAOSMQ010000490         2455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_42781_length_2455_cov_3.835417, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000490 DAOSMQ010000000
VERSION     DAOSMQ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42781_length_2455_cov_3.835417"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK829_13195"
     CDS             <1..610
                     /locus_tag="PK829_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOU42215.1"
                     /translation="FGLDRTLTTGVVSALGRPLETDTGDYIFNVIQTDAAINPGNSGG
                     PLLNSRGEVMGINTAIQEGAQGIGFAVPAATLRRVVPVLLAEGAYQHATLGLLGYSIN
                     AGLAEALGLPVEQGVLVAQLAQGGPADAAGVRGAQQEVIVGNRRVLAGGDIITALDGN
                     AINDWNALLEYLELHKAVGDTIVLSLLRDGQPLTIQATLGAE"
     gene            926..1381
                     /locus_tag="PK829_13200"
     CDS             926..1381
                     /locus_tag="PK829_13200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU42216.1"
                     /translation="MTYTNYPYEIALEVAFRDIDAMGHVNNAVFFAYFETVRIKYLGE
                     VMREGILGSELLDLPLILVNASCTYKSPALLTEVLHIGTGISRFGTKSFDMLYQVQGE
                     DGRLVAYGKTVQVMYDYITREAFPIPDELRRFIAGYQSDWQPPAYPEYL"
     gene            complement(1389..>2455)
                     /locus_tag="PK829_13205"
     CDS             complement(1389..>2455)
                     /locus_tag="PK829_13205"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU42217.1"
                     /translation="ATVTMRHLLTHMGGWLGDFFRETGSGDDALARYVEEMATLEQVV
                     PVGTLWAYNNAGFGLAGRVIEKVTGKGYETVAQEMVLDPLGMANSFFFPADVISRRFA
                     VGHSVHEGQGPVVERPWALARSAHAVGGLCSSARDQMRYARFHLGDGATETGQRLLAA
                     GTMAAMQSPQCAANLERQMGLSWILRDMAGTPTVLHGGATNGQQSAFVMAPSRRFAIT
                     VLTNADEGNMLHDAVVAWAMERYLGLVASEVDQAQPDADALAAYTGLFRARLSHVELT
                     VQDGVLMGRMIPQGGFPDVDSPASPPTPFSRVVFYAPDRIVALDPPLKGLKAEFLRDE
                     AGQIAWLRTSRLMRRDEPAA"
BASE COUNT          404 a          833 c          802 g          416 t
ORIGIN      
        1 gtttggcctt gaccggacgc tgacgacggg tgtggtcagc gcgctgggcc ggccgttgga
       61 gactgacacg ggcgactaca tcttcaatgt catccagacg gacgcggcta ttaatcccgg
      121 caactccggc gggccgctgt tgaactcgcg cggcgaggtc atgggcatca acacagccat
      181 ccaggagggc gcgcagggca ttggcttcgc cgtgcccgcg gccacgctgc ggcgcgtggt
      241 gcccgtcttg ctggccgaag gggcctatca gcatgcgacg ctgggcttgt tgggttactc
      301 gattaacgcc gggctggccg aggcgttggg gctgccagtg gagcaggggg tgctggtggc
      361 ccaactggcg cagggcggcc cggccgatgc cgccggcgtg cgcggcgcgc agcaggaggt
      421 catcgtcggt aaccgccgcg tcctggccgg cggcgacatc atcaccgcgc tggacggcaa
      481 cgccatcaat gactggaacg cgctactgga gtacctggaa ctgcacaagg ccgtcggcga
      541 caccattgtc ttgtcgctcc tgcgcgacgg ccaacccctg accattcagg cgacgctggg
      601 cgctgagtag atggccggcg gacaacggac gacggcccac cgccgacctc atctctcgtc
      661 tcgtctggcc gccgtgtgtg ggcgttggtc tgtcgtccct cggccaccag gactttgcca
      721 aagtcgtctg gcgaccattg acctgaccgg tggacggcca gttgttctct agtggcagta
      781 ccatgcgtgg ccgcccatgt caggtcgatg cgccggcggt ggctttggca aagccctgcc
      841 tcggccacgc cctttctttc cgccgccaga caaacgtgct agaatgcccc tgtcattccc
      901 acagtcagcc catctccgag acagcatgac ctacaccaac tacccctatg agatcgccct
      961 ggaggtcgcc ttccgcgaca tcgatgccat gggccacgtc aacaacgccg tctttttcgc
     1021 ctacttcgag acggtgcgca tcaagtacct gggcgaagtg atgcgcgagg gcatcctcgg
     1081 ctcggaattg ctcgatctgc cgctcatcct cgtcaacgcc agttgcacct acaagtcgcc
     1141 ggcgttgcta accgaggtgc tccacatcgg tacgggtatc tcccgcttcg gcacgaagag
     1201 cttcgacatg ctctaccagg tgcagggcga ggacggccgc ctggtggcct acggtaagac
     1261 ggttcaggtg atgtacgact acatcacccg tgaggccttc cccatccccg acgagctacg
     1321 ccgcttcatt gccggctacc agagcgattg gcagccgccc gcgtaccccg aatacctcta
     1381 gccgaggctc aggcagccgg ttcgtcgcgc cgcatgaggc gactggtgcg cagccaggcg
     1441 atctggccgg cctcgtcgcg taggaactcg gctttcagcc ccttcagcgg cgggtcgagg
     1501 gcgacgatgc gatccggcgc gtagaagacg acccggctga acggcgttgg cggcgaggcg
     1561 ggcgagtcca catccgggaa gcctccctgc ggaatcattc tgcccatgag cacaccgtcc
     1621 tgcaccgtta gctcgacgtg cgacagccgc gccctgaaga ggccagtata cgcggccagc
     1681 gcgtcagcgt ccggctgcgc ctggtctacc tctgacgcga ccagccccag atagcgctcc
     1741 atcgcccagg ccacaacggc atcgtggagc atgttgccct cgtcggcatt ggtcagcacg
     1801 gtgatggcga agcggcgcga gggggccatg acgaaggctg actgctgccc gttggtcgcg
     1861 ccaccgtgca gcaccgtcgg cgtgccggcc atgtcgcgca gtatccagct caggcccatt
     1921 tgacgctcca ggttggccgc gcattgcggt gactgcatgg cggccatcgt cccggccgcg
     1981 agcagccgct ggcccgtctc ggtcgcgccg tcgcccaggt ggaagcgggc atagcgcatc
     2041 tggtcgcgag cgctggagca caggccgccg acagcatggg ccgagcgggc cagcgcccac
     2101 ggccgctcca cgaccggccc ctggccctca tggacgctgt ggccaacggc gaagcggcgc
     2161 gagatcacgt cggccggaaa gaagaaggag ttggccatgc ccagcgggtc gagcaccatc
     2221 tcctgggcga cggtctcata gcccttgccg gtgaccttct cgatgacccg cccggccagg
     2281 ccgaagccgg cgttgttata agcccacagc gtacccacgg gcacaacctg ttcgagtgtg
     2341 gccatctcct cgacgtagcg ggccagggcg tcgtcgccgc tgcccgtctc gcggaagaaa
     2401 tcgcccagcc agccgcccat gtgggtcagc aagtggcgca tggtgacagt ggcct
//
LOCUS       DAOSMQ010000491         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_43192_length_2436_cov_4.418732, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000491 DAOSMQ010000000
VERSION     DAOSMQ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43192_length_2436_cov_4.418732"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK829_13210"
     CDS             <1..277
                     /locus_tag="PK829_13210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42218.1"
                     /translation="FGFPVLEAQACGTPVVCSNTSSLPEVAGEGALLVDPRDTAALAA
                     ALRRVAGEDDLRRALIRAGYANGPRFTWAAAARQTLAMLLQVAGQVN"
     gene            complement(300..716)
                     /locus_tag="PK829_13215"
     CDS             complement(300..716)
                     /locus_tag="PK829_13215"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU42219.1"
                     /translation="MKISARVQSSFGQQIITLATNENSHTIDIPPKPTGYGSSANGGE
                     LLFLALATCYCNDIYREAARRGIEVTGVEVDVRGDFGAEGEPGRNVTYQARITGKATA
                     AALEDLARHTDTVAEIQNTLRAGATVSLSEVEVLPV"
     gene            complement(755..1540)
                     /locus_tag="PK829_13220"
     CDS             complement(755..1540)
                     /locus_tag="PK829_13220"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU42220.1"
                     /translation="MRENKSPDVGSIWYFWFATDAFHVDKRLRRLNRRLPSEPRCRFC
                     NAPFRGMGGALERMLFGKRQSSLNPRYCNMCDEASRRFPGGAEVPMSMLFADVRGSTA
                     LSETMSPMAFSRLINRFYTRAARIIGEEDGLVEKLAGDSVAAFWGAGFAGPDYVARTV
                     AVAQQLVRAMTEEEIPIGVGVHAGVAYFGAVGTEEGLTEFAALGDEVNLAARLAAQAA
                     AGEIIISQTALAAANVPADGLETRELELKGISRPVSAYVLHAA"
     gene            1601..>2436
                     /locus_tag="PK829_13225"
     CDS             1601..>2436
                     /locus_tag="PK829_13225"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOU42221.1"
                     /translation="MQTELIHPGPLLDTAGRLAQVGWARQPLLDANLEKARFYALRPL
                     QRFRLKRWDYYALFTPRGFFSATVADLGYAANVFVYTLDYANGELYEEGLVVPLARGV
                     GLARGSGEGETAYQSDKASLRFTAGPDGRRVRVDWPGFHGGRGIAADIHLATPPGHES
                     VTIVIPIGARRFYYNTKINCLPAAGTLRHGDAVEELSPTTSLGSLDWGRGVWEYRSYW
                     NWASASGFLPDGRTVGLNLGRGFGDLSRATENAIVLDGRVHKLGDVRFDYQPGAYMRP
                     WR"
BASE COUNT          374 a          821 c          819 g          422 t
ORIGIN      
        1 ctttggtttc ccggtgctgg aggcgcaggc gtgcggcacg ccggtggtgt gcagcaacac
       61 aagttcgctg ccggaggtgg ccggggaagg ggcgttgctg gtcgatccgc gcgacacggc
      121 ggcgctggcg gcggcgctgc ggcgcgtggc cggcgaggat gacctgcgcc gcgcgttgat
      181 ccgcgcgggc tatgccaatg gcccacggtt tacgtgggcc gcggcggcga ggcagacatt
      241 ggctatgctt ctccaagtgg caggccaggt caattaggca gtcaaacagc ctccgctgct
      301 tacaccggca ggacctcgac ctcgctcagg ctgacggtcg cgccggcgcg caaggtgttc
      361 tggatttcgg cgacggtatc ggtgtggcgg gccaggtctt ccagcgcggc ggccgtggct
      421 ttgccagtga tcctggcctg gtaggtgacg ttgcggcccg gttcgccctc ggctccgaag
      481 tcgccgcgca cgtcaacctc cacgcccgtc acttcaatgc cgcgcctggc cgcctcgcgg
      541 tagatgtcgt tgcaatagca ggtggccagc gccaggaaga gcaactcgcc accgttggcg
      601 ctggagccgt agccggttgg tttgggcggg atgtcgatgg tgtggctgtt ttcgttggtc
      661 gccagagtaa tgatctgttg gccaaagctg ctttggacgc gcgcactgat cttcatgttg
      721 ggtctccgca tggatatagt tgcccagcac agcgctaggc ggcatgcaac acataggccg
      781 acaccggccg gctgatcccc tttaactcaa gctcacgggt ctccagcccg tcggccggca
      841 cgttggccgc ggccagagcc gtctggctga tgatgatctc gcccgccgcg gcctgggcgg
      901 ccaggcgcgc ggccaaattg acctcatcgc ccagggcggc gaactcggtc agcccctcct
      961 ctgtgcccac cgcgccaaag taagccaccc cggcgtgaac gccaacgcca atgggtattt
     1021 cctcctcggt catggcgcgg acgagctgct gcgccacggc caccgtccgc gccacatagt
     1081 ccggcccggc gaagcccgcg ccccagaagg cggccacgga gtcgccggcc agcttttcca
     1141 ccagcccatc ttcctcgccg atgatcctgg ccgcgcgagt atagaagcgg ttgatgaggc
     1201 gactgaacgc catcggactc atggtctccg agagcgctgt cgagccgcgc acgtcggcga
     1261 agagcatcga catgggcacc tccgccccgc cggggaagcg gcgcgaggcc tcgtcgcaca
     1321 tgttgcagta gcgcgggttg agcgatgact ggcgcttgcc gaagagcatg cgctccaacg
     1381 cgccgcccat gccgcggaaa ggcgcgttgc agaaccggca acgcggttca ctcggcagtc
     1441 tccgattcag ccgtcgcagg cgcttgtcaa cgtgaaaggc gtcggtcgcg aaccagaaat
     1501 accagatgct accgacgtca ggactcttgt tctctctcaa gtcgatcctt ccttcccctc
     1561 cgcgccaatc ggtgctttaa tccgggccaa aggatagcac atgcaaaccg aactgatcca
     1621 tcccggcccc ttgctcgata ccgccggccg cctggcgcag gtgggctggg cgcggcaacc
     1681 gttgttggac gctaacctgg agaaagcgcg cttctacgct ttgcggccgt tgcagcgctt
     1741 ccgcctgaag cgctgggact actacgccct ctttacgccg cgcgggtttt tctccgccac
     1801 ggtcgccgac ctgggctatg cggccaatgt ctttgtctac acgctcgact acgccaacgg
     1861 cgaactctac gaggaggggc tggtcgtccc gctggcgcgt ggcgtggggc tggcgcgcgg
     1921 cagcggcgaa ggcgagacgg cttaccagag cgacaaggcg tcgctccgct tcaccgccgg
     1981 gccggacggg cggcgcgtgc gcgtcgattg gcccggcttc cacggcggcc ggggcattgc
     2041 cgccgacatc cacctggcca cgccgccggg ccacgagtcc gtgaccatcg tcatccctat
     2101 cggcgcgcgg cgcttctact acaacaccaa gatcaactgc ctgcccgccg ccggcacgct
     2161 gcgccacggc gacgcggtcg aggagttgtc gcccacgacc agcctcggct cgctcgattg
     2221 ggggcggggc gtgtgggagt atcgcagcta ctggaactgg gccagcgcgt cgggcttcct
     2281 gccggatggg cgcacggttg ggctgaacct cggccgcggc ttcggcgacc tgagccgggc
     2341 cacggagaac gccatcgtcc tcgacggccg cgtccacaag ctgggcgacg tgcgcttcga
     2401 ttaccagccg ggggcgtaca tgcggccgtg gcgctt
//
LOCUS       DAOSMQ010000492         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_43201_length_2436_cov_3.066779, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000492 DAOSMQ010000000
VERSION     DAOSMQ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43201_length_2436_cov_3.066779"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK829_13230"
     CDS             <1..538
                     /locus_tag="PK829_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42222.1"
                     /translation="DDHVGAAVGRAGGRGRRWQINTNAFPARYVTFPVYSGQRAMQIG
                     IIRPQDNVYSYSSTQQTLFIPAGPPSVALSFQLFATTTGTRAGSLTPPPVVPTSPLDR
                     ARLSDDAQMVLLFDSRGQQHVLLFQRQWYSQWQRHVIDLSAFRGQAVTLYFGVFNNGT
                     GGITGMAVDDVAVTYCLP"
     gene            complement(541..>2436)
                     /locus_tag="PK829_13235"
     CDS             complement(541..>2436)
                     /locus_tag="PK829_13235"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU42223.1"
                     /translation="EDAALAHRLATALREAGRPVWIAPDDILPGEQWVEAIGRGLTVS
                     GLFVILITPQAVQSEWVKYEVNLAITLERRLRMEIVPLEVVPAETPITWSAYQAIEFG
                     DYDADLPHVLARLAARRRAPEARPPSPALPPPEPPRRTAALNQPAPLPLPASAPVVLP
                     DHVPQNYPRQPTYRIHAPTEMALAHVLAGEFLYGPERQPAQAGEFWIGRYQVTNADYK
                     RFIDANPGHPVPHVAADWAKPYNWNPTTRDYPPGKATHPVVLVRWADAKTFCDWAGMR
                     LPTEEEWEKTARGVDGRAYPWGDEPPTADLCNCDHATLGTSPVGRYSPQGDSPYGCGD
                     MAGNVSEWTSTPGNADGMRIVRGGAWPFASQNNQVTSRLEAAAERQTPYIGFRALTDS
                     PTDTPTGERWTHLRSGIEFLRVPAGQFFFGDSRQPVDLDAFWIGRYEVTNLQFDHFVR
                     ATGYRTVAEQKGYGRVLRDNKWVRLPGAYWRQPDGPDSAIDNRWQHPVVQISAADADA
                     FAQWAGLRLPTEEEWEKAARGGDSRVFPWGNRAPSPRLLNYGRLMGNTSPVGRYSPDG
                     DSPYGVADMAGNVWEWTTTRYDRDPDLLVLKGGSWADPETSWLRGAAGPRHCQASIGF
                     RLVTSAS"
BASE COUNT          362 a          834 c          865 g          375 t
ORIGIN      
        1 tgacgaccac gtcggcgcag cggtcgggcg ggcgggtggc cgtgggcgac gctggcagat
       61 caacaccaac gccttcccgg cccgctacgt cacctttccg gtctacagcg gccaacgcgc
      121 catgcaaatc ggcatcattc gcccgcagga caacgtctac agctactcat cgacccagca
      181 gacgcttttc atccccgccg ggccgccgtc ggtggcgctc agcttccagc ttttcgccac
      241 gacgacgggc acgcgggccg gaagcctgac accgccaccg gtcgtgccca cttcgcccct
      301 cgaccgggcg cggctgagcg acgacgcgca aatggtgctg ctgttcgaca gcagagggca
      361 gcaacacgtc ttgctcttcc agcggcagtg gtacagtcaa tggcagcggc acgtcatcga
      421 cctgagcgct ttccgcggcc aggcagtgac gctctacttc ggggtcttca acaacggcac
      481 gggcgggatc accggcatgg ccgtggacga cgtggccgtg acctactgcc tgccgtaggg
      541 ctaggaagcc gaggtcacca gccggaagcc gatcgacgcc tggcagtggc gtgggccggc
      601 cgcgccgcgc agccaggagg tctccgggtc ggcccaggaa ccgcccttca acaccagcag
      661 gtcggggtcg cggtcatagc gcgtcgtcgt ccactcccag acgttgccgg ccatgtcggc
      721 cacgccatag gggctgtcgc cgtcgggcga gtagcggccc acgggcgatg tgttgcccat
      781 cagccggcca tagttgagca ggcgcggcga gggcgcgcgg ttgccccagg ggaacacgcg
      841 gctgtcgccg ccgcgcgcgg ccttctccca ctcctcctcg gttggcagcc gcagcccggc
      901 ccattgggca aaggcgtcag cgtcggcggc gctgatctgc acgaccgggt gttgccagcg
      961 gttatcaatg gccgagtctg gcccgtccgg ctgtcgccag taggcgccgg gcagtctcac
     1021 ccacttgttg tcgcgcagca cgcgcccata gcccttctgc tcggccactg tccggtagcc
     1081 ggtggcgcgc acgaagtggt cgaattgcag gttggtgacc tcgtagcggc cgatccagaa
     1141 ggcatccagg tccaccggct ggcgactgtc gccaaagaag aactgcccgg cgggcacgcg
     1201 cagaaactcg atgccgctgc gcagatgtgt ccagcgctcg ccggtgggcg tgtcggtggg
     1261 cgagtcggtc agggcgcgaa agccgatgta gggtgtctgg cgctcagcgg cggcctccag
     1321 ccgggaagtg acctgattgt tctgcgaggc gaagggccag gccccgccac ggacaatgcg
     1381 catgccgtcc gcgttgccgg gcgtgctcgt ccactcgctg acgttgccgg ccatgtcgcc
     1441 gcagccatag gggctatcac cctggggcga atagcggccg acgggcgacg tacccagcgt
     1501 ggcgtggtcg caattgcaga gatcggccgt cggcggctcg tcgccccagg ggtaggcgcg
     1561 gccgtccacg ccccgcgccg tcttctccca ctcctcctcg gttggcagcc gcatgccggc
     1621 ccagtcgcaa aacgttttgg cgtcggccca acgcaccagc accaccgggt gggtcgcctt
     1681 gccgggcgga tagtcgcgtg tggtcgggtt ccagttatag ggcttggccc aatcggcggc
     1741 tacgtggggc acagggtgac cggggttggc gtcgatgaag cgcttgtagt cggcgttggt
     1801 gacctgatag cgcccgatcc agaactcgcc cgcctgcgcc ggctgccgct ccgggccata
     1861 gagaaactcg cccgccagca cgtgggccag ggccatctcc gtcggcgcgt ggatgcgata
     1921 ggtgggttgg cgcggatagt tctgcggcac atggtcgggc aagaccacgg gcgcggacgc
     1981 cggcaggggc agcggcgcgg gctggtttag cgcggccgtg cgccgcggcg gctctggcgg
     2041 cggcaaggcc gggctgggcg gacgcgcctc cggcgcgcgc cggcgcgccg ccagccgtgc
     2101 cagcacatgg ggcaaatcgg cgtcgtagtc gccgaattca atggcctgat aggccgacca
     2161 ggtgatcggc gtctccgccg gcacgacctc caacgggacg atctccatgc gcaggcggcg
     2221 ctccagggtg atggccagat tgacttcgta cttgacccac tccgactgca ccgcctgggg
     2281 ggtgatcagg atgacgaaca ggccgctgac ggtcaggccg cggccgatgg cctcaaccca
     2341 ctgctcgccg ggcaggatgt cgtccggggc gatccacacc gggcggccgg cctcgcgcag
     2401 ggccgtggcc agccggtggg ccagcgcggc gtcctc
//
LOCUS       DAOSMQ010000493         2411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_43799_length_2411_cov_4.953735, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000493 DAOSMQ010000000
VERSION     DAOSMQ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43799_length_2411_cov_4.953735"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PK829_13240"
     CDS             complement(<1..801)
                     /locus_tag="PK829_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOU42224.1"
                     /translation="MDFDVKNIDLAPGGRHRIEWAEQEMPVLRGIQQQFKDERPFAGL
                     RVSACMHVTTETANLMASLHQGGADVVLCASNPLSTQDDVAAALVTHYEIPVYAIKGE
                     DNETYFKHIHAVLDHNPHIVMDDGADLVSTIHKDRRNLIDNMLGGTEETTTGIIRLRA
                     MARDGALQFPMMAVNDALTKHMFDNRYGTGQSTMDGIIRATNILLAGRNVVVAGYGWC
                     SRGIAMRARGLGANVIVTEIDPLKALEAVMDGYRVLPMIEAAAIGDIFV"
     gene            complement(844..1839)
                     /locus_tag="PK829_13245"
     CDS             complement(844..1839)
                     /locus_tag="PK829_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU42225.1"
                     /translation="MKIVITGSIAYDYLMSFPGKFTDAFVADKLEKISLSFLVDSLKR
                     QRGGTAPNIAYTLALLGDRPLIMATAGQDFGEYRAWLESQGVDTSAIIDIDGEFCASF
                     FVNTDEDHNQIASFYTGAMAHAGRLSFAQYAPDADLTIITPNDPGAMRAYVRECKAIG
                     LPYIYDPSQQTLRVTGEELCEGLDGCKLLSLNEYEFSLVREKTGLSEADILSRAGGLV
                     MTMGATGSRLVVDGRELFIPPVTPRVLAEPTGAGDAYRAGLLRGMQLGAPWEVAGRMG
                     ALAAAYVLEQFGPQNHTFTRAEFVARYRQHFDDEGALDALLAIPESIPSPLPVAP"
     gene            complement(2060..>2411)
                     /locus_tag="PK829_13250"
     CDS             complement(2060..>2411)
                     /locus_tag="PK829_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42226.1"
                     /translation="TMTNLLFGGHLTDVATATKMVRADVAQSLNLTTTDFNLDFELPD
                     KILLAGHDIVEIPISYNPRTYEEGKKIRTRDGLRALLTMVRDRLGLTPVLKPPAAVGA
                     SGREMPARGPGGAL"
BASE COUNT          394 a          763 c          807 g          447 t
ORIGIN      
        1 tacgaaaatg tcgccaatgg cggcggcctc gatcatcggc aagacgcggt agccgtccat
       61 gaccgcttcc agcgccttca gcgggtcgat ctcggtgacg ataacgttgg cccccaggcc
      121 acgggcgcgc atggcgatgc cgcggctgca ccagccgtag ccggcgacga cgacgttgcg
      181 gccggccagc aggatgttgg tcgcccggat aatgccgtcc atcgtcgatt ggcccgtgcc
      241 atagcgattg tcgaacatgt gcttggtcag cgcgtcgttg acggccatca tggggaactg
      301 cagcgcgccg tcgcgggcca tggcccgcag gcgaatgatg cccgtggtcg tctcttccgt
      361 gccgccgagc atgttgtcga tcaggttgcg gcggtctttg tggatggtgc tgaccagatc
      421 ggcgccgtcg tccatgacga tatggggatt gtggtcgagc acggcatgga tgtgcttgaa
      481 gtaggtctcg ttgtcctcgc ccttgatggc gtagaccggg atctcgtagt gggtgacgag
      541 ggccgcggcc acgtcgtcct gggtgctcag cgggttgctg gcgcagagaa cgacatcggc
      601 cccgccctga tgtaggctgg ccatcaggtt ggccgtctcg gtagtcacgt gcatgcaggc
      661 gctgacgcgc agcccggcga aggggcgctc atccttgaat tgctgctgga tgccgcgcag
      721 cacgggcatc tcctgctcgg cccactcgat gcgatgccgg ccgccgggag ccagatcgat
      781 gttcttgacg tcgaagtcca tgtgtgcaga ttctccttaa acaactggcc ttgttgccgc
      841 cgcctacggg gcgaccggca aaggcgatgg aatggattcg gggatggcga ggagcgcgtc
      901 gagcgcgccc tcgtcgtcga aatgctggcg atagcgggcc acgaactcgg cccgcgtgaa
      961 ggtgtggttc tgcgggccga actgctccag cacgtaggcg gccgccagcg cgcccatgcg
     1021 cccggccact tcccagggcg cgcccagttg catgccgcgc agcagtccgg cgcggtaggc
     1081 gtcgcccgcg cccgtcggct cggccaacac gcgcggcgtc acgggcggga tgaacaactc
     1141 gcgcccatcg accaccagcc gcgagccggt cgcgcccatg gtcatcacca gcccgccggc
     1201 gcggctcagg atgtcggcct cgctcaggcc cgttttctcg cgcaccaggc tgaactcata
     1261 ctcgttgagc gacaacagct tgcagccatc cagcccctcg catagctcct cgccggtgac
     1321 gcgcagagtc tgctggctgg gatcatagat gtagggtagg ccgatggcct tgcactcgcg
     1381 gacgtaggcg cgcatcgccc cggggtcgtt gggagtgatg atggtcaggt cggcatcggg
     1441 ggcatattgg gcgaaagaca gccgcccggc gtgggccatc gccccggtgt agaagctggc
     1501 gatctggttg tggtcttcgt cggtgttgac gaagaaggag gcgcagaact cgccgtcgat
     1561 gtcgatgatg gccgaggtat cgacgccctg gctttccagc caggcgcggt attcgccaaa
     1621 gtcctgcccg gccgtggcca tgatgagcgg ccggtcgccc aacagggcca gcgtgtaggc
     1681 gatgttgggc gcggtgccgc cgcgctggcg cttcagcgaa tcaaccagaa agctcaggct
     1741 gatcttctcc agcttgtcgg ccacaaaggc atcggtgaac ttacctggaa aggacatcag
     1801 gtagtcatag gcgatggagc cggtgatgac gatcttcata gctgcaatgt ctctctttcc
     1861 ccccatcagg aggatcgtgc cggcggcctc acgtgatcag acggcgcatc gcgaccctct
     1921 cggcccgatc gccgcgcatc tcgattatgg acgaacggtc acgctgcgag tgttctacag
     1981 cggcgcaacg ctccagtata ccctgtttgc gccgcgacgc accccgatgg actataggct
     2041 cccccggtta cgatctagct tacaatgcgc cgcccggccc acgggcgggc atttctcggc
     2101 cggaagcccc gacggccgcc ggcggcttca gcaccggcgt cagccccagc cggtcgcgca
     2161 ccatcgtcag cagcgcccgc agcccgtcgc gcgtgcggat cttcttgcct tcctcgtagg
     2221 tgcgagggtt ataactgatg ggaatctcca caatgtcgtg gccggccagc aagattttat
     2281 ccggcaactc gaagtcgagg ttgaaatcgg tcgtcgtcag gttcagcgat tgggccacgt
     2341 cggcgcggac catcttggtc gccgtggcca cgtcggtcag gtgaccgccg aagagcaggt
     2401 tggtcatcgt c
//
LOCUS       DAOSMQ010000494         2372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_44850_length_2372_cov_3.924471, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000494 DAOSMQ010000000
VERSION     DAOSMQ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44850_length_2372_cov_3.924471"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK829_13255"
     CDS             <1..416
                     /locus_tag="PK829_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOU42227.1"
                     /translation="AWFEESLASGKRIMGIGHRIYKVEDPRAKILKPLAEKLAKSSGQ
                     TQWYQVADTIQQLARQNDYFIERNLYANVDYYSAVVLYTIGLPVDQFTCIFAVSRIAG
                     WCSQVLEQLADNRLIRPKESYVGKWDQSFVPLAER"
     gene            509..1849
                     /locus_tag="PK829_13260"
     CDS             509..1849
                     /locus_tag="PK829_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOU42228.1"
                     /translation="MNRAIAFSIIVLAVALAACSAGGGEPTAGTATDTPATSLAQAPA
                     PAPPIVATNTYIPLVEDQPTPTATRPLPTATPSPLPTATPLSPVAAVALEPVLAGGLQ
                     HPTAITHAGDDRLFVLEQIGRIRIVAGGQLVATPFLDITDRVGSAGSEQGLLGLAFHP
                     NYATAGAPGAGTFYVNYTDYGGATHISRFTVRADDPNRADPNSEVIYLTQPQPYPNHN
                     GGSLAFGPDGYLYAGLGDGGSANDPQGAGQRRDTWLGKLLRLDVTTNDGQYTVPPDNP
                     FVGQPQARPEIWAYGLRNPWRFSFDRATGDIFIADVGQNRWEEVNFQPASSAGGENYG
                     WNIMEATHCFQQETCDRDGLALPIFEYSHSQGCSITGGYIYRGAAYPQLTGNYFVADY
                     CLGTIWRLFPQIDSWQADVVLDSDLAISTFGQDVDGELYVADYANGAIYRLRPN"
     gene            complement(1846..2196)
                     /locus_tag="PK829_13265"
     CDS             complement(1846..2196)
                     /locus_tag="PK829_13265"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOU42229.1"
                     /translation="MPLFKRAPAADAEGYVATVPVAAVTEDEMTIIRMAGKKVILTRR
                     AGHVYAIDSVCPHGAADLSEGWLRRGRLCCHEHEYCFDVATGLLVWPDDEPYRLRRYD
                     VKEEDGVVKVRPAR"
BASE COUNT          424 a          828 c          734 g          386 t
ORIGIN      
        1 gtgcctggtt tgaagaatct ctggcctccg gcaagcgcat tatgggcatc ggccatcgca
       61 tctacaaggt cgaggacccg cgcgccaaaa tcctgaagcc gttggccgag aaactggcca
      121 agagcagcgg ccaaacccag tggtaccagg tcgccgacac cattcagcag ctcgcgcgcc
      181 agaacgacta cttcattgag cgcaacctgt acgccaacgt cgactactac tcggccgtcg
      241 tcctctacac catcggactg ccggtcgatc agttcacctg cattttcgcc gtcagccgca
      301 tcgccggctg gtgctcgcaa gtgctggagc aactggccga caaccgcctg atccggccca
      361 aggaaagcta cgtcggcaag tgggatcagt ctttcgttcc gctggccgag cgctagccgc
      421 ttaccgcggc cggcggggag cctaaagtga catgagaacc ggccgctggc gcaagtcgcc
      481 gcggccggtt cttctataat cacggcctat gaaccgagcc atcgctttta gcatcattgt
      541 cctcgctgtc gcattggccg cctgttcggc gggtgggggc gagccgaccg ccgggacggc
      601 gacggacacg ccggccacct ctctggccca agcgccggca cccgcgccgc cgattgtagc
      661 caccaatacc tacatccccc tcgtcgaaga ccagccgacc cccacggcga cacgtccgct
      721 gcccacagcc acgccatcgc ccctgcccac ggcgacaccg ctcagccccg tcgccgccgt
      781 ggccctggag ccggttctgg ccggcggtct ccagcacccg acagcaatca cccacgccgg
      841 tgacgaccgg ctcttcgtgt tggagcagat cggccgcatc cgtatcgtcg ccggtggcca
      901 gctggtggcg acgccgtttc tggacattac cgaccgcgtt ggctccgccg gcagcgagca
      961 ggggctattg gggttggcct tccatcccaa ctatgccacg gccggcgcgc cgggcgcggg
     1021 cacgttctac gtcaactata ccgactacgg cggcgcgacc cacatctcgc gcttcaccgt
     1081 ccgcgccgat gaccccaacc gcgccgaccc gaacagcgag gtcatttacc tgacccagcc
     1141 acagccctac cccaaccaca acggtggcag cctggccttt ggccccgacg gctaccttta
     1201 tgccggcctg ggcgatggcg gcagcgccaa cgacccacag ggagccgggc agcggcgcga
     1261 cacctggctg ggcaagctgc tgcgccttga cgtgaccacg aacgatggcc agtacaccgt
     1321 gccgccggat aacccgttcg ttggccagcc acaagcgcgg cccgaaatct gggcctatgg
     1381 cctgcgcaac ccgtggcgct tctccttcga ccgggccaca ggcgacattt tcatcgccga
     1441 cgtgggccag aaccggtggg aagaggttaa cttccagccg gccagcagcg ccggtggcga
     1501 gaactacggc tggaacatca tggaggcgac acactgcttc caacaggaga cgtgcgaccg
     1561 cgacgggctg gcactgccca tcttcgagta tagccacagc cagggctgct ccatcaccgg
     1621 cggctacatc tatcgcggcg cggcctaccc gcagctgacc ggcaactact tcgtggccga
     1681 ctactgcctg ggcacgatct ggcggctgtt cccgcagata gacagctggc aggccgacgt
     1741 cgttctcgac agtgacctgg ctatctcgac atttggccag gacgtggacg gcgagctata
     1801 cgtcgccgac tacgccaacg gggccatcta ccggctgcga ccgaattagc gcgccggacg
     1861 caccttgacg acgccgtcct cctctttcac atcgtagcgg cgcaggcggt agggttcgtc
     1921 gtccggccag acaagcagac cggtggcaac atcgaagcag tactcgtgct cgtggcagca
     1981 caggcggccg cggcgcagcc agccctcgct gagatcggcc gcgccgtggg ggcagacgct
     2041 gtcgatggcg tagacgtggc cggcgcgacg ggtaaggatg acttttttgc cagccatgcg
     2101 gatgatggtc atctcgtctt cggtgacggc tgccacgggg acggtggcca cgtaaccctc
     2161 ggcgtcggcg gccggggcgc gcttgaaaag tggcatggcc acggttatag ccgagtcata
     2221 cgcgaccggc aatcccggtg gatagaggcg cagcgatgac aacgatcctc attcttggcg
     2281 gctatgggca ggccggccgc gcgctggccg actacttgct ggcgcagacc gattgtcgtc
     2341 tcatcctagc cgggcgacgg ccagagcggg ct
//
LOCUS       DAOSMQ010000495         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_44951_length_2368_cov_4.060960, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000495 DAOSMQ010000000
VERSION     DAOSMQ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44951_length_2368_cov_4.060960"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="PK829_13270"
     CDS             <1..1551
                     /locus_tag="PK829_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU42230.1"
                     /translation="IRLTVDSNDAEAAYRQVYRQLRAAILAGELPPGSRLPATRTLAR
                     ASGVARVTVVEAYRQLEAEGFTESRVGAGTFVAAGVVATGGVAAGGVATGGVAAGGVA
                     AERAAAGRAIAPGDGPVAFRPALTEWGRRAVEVGHSAPASDGGRPEIDFGLGRAFAEL
                     FPYHVWRRLLARYLSTDDAMLSRYGSPAGFFPLRQAVADYLGRARGVRCVAEQVVIVS
                     GAQQALDILARLLLNPGDEVVVETPGYRDAFTLFQLHGARLVGLPVDEEGLPVDRIPA
                     DCRARLAFVTPTHQFPKGGTMPLARRMALLAWARAGGALVIEDDYDGDLRYDGHSLAA
                     LQGLDAAGADTDGRVAYLGTFSKVLFPALRLGYLVLPPPLVTPFVRAKDLVDRGAPTL
                     TQAAVADFIAEGHFERHLRRLRRLYGQRRAALGAALTARLGERVHFSPLAAGLHVMLF
                     PEANVDEARLVREAAARGVRVHPGAPYHLERPAPPSILLGFSGLSETEIVEGVRRLAE
                     AWPVAGAA"
     gene            complement(1559..2077)
                     /locus_tag="PK829_13275"
     CDS             complement(1559..2077)
                     /locus_tag="PK829_13275"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOU42231.1"
                     /translation="MVTLGRLDNTPIGTIWAAAADSTLLAVSLWDDPTRLADDAARLT
                     GETPDLAAPPGPIVIAALAQLNEYLRGERRVFDLPLPWPALTPFAREVLTRVYAVPYG
                     QTSSYGAIAAEMGRPGAVRAVGQANANNPIPIIIPCHRVLGTDGKLHGYGARGGLETK
                     AWLLRLEGSWLV"
     gene            complement(2052..>2368)
                     /locus_tag="PK829_13280"
     CDS             complement(2052..>2368)
                     /locus_tag="PK829_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU42232.1"
                     /translation="ARLRDEGFCLWALERTAGASPQPSLYDAGPPDAPLALVVGNERA
                     GVDPGLLALCDGVFSLPMYGGKSSLNVAVAFGIAVYHLRFAAAPQAEIEGVSHGDTGT
                     TG"
BASE COUNT          295 a          835 c          861 g          377 t
ORIGIN      
        1 atccggctga ccgtggattc caacgacgcc gaggccgcct accggcaggt ttatcgccaa
       61 ctgcgggcgg ccattttggc gggcgagttg ccgccgggca gccgcttgcc ggcgacgcgc
      121 accctggccc gcgccagcgg tgttgcccgt gtcaccgtcg tcgaggccta ccggcagttg
      181 gaggccgagg gtttcaccga gagccgcgtc ggcgcgggga cgtttgtggc cgctggcgtg
      241 gtcgccaccg gcggggtcgc cgctggcggg gtcgccaccg gcggggtcgc cgctggcggg
      301 gtcgccgccg agcgggctgc cgctggacgg gccatcgcgc ccggcgatgg cccggtcgcc
      361 ttccggccgg cgctgacgga gtggggccgc cgcgcggtcg aggtcggcca ttctgcgccg
      421 gcctctgacg gaggccggcc ggagatcgac ttcggcctgg gccgcgcctt tgccgagttg
      481 tttccctatc acgtctggcg gcgcctgctg gcccgctacc tgtccaccga cgacgccatg
      541 ctctcgcgct acggctcgcc ggccggcttc ttccccttgc ggcaagccgt ggccgactac
      601 ctggggcggg cgcggggcgt gcgctgcgtc gccgagcagg tggtcatcgt cagtggcgcg
      661 cagcaggcgc tcgacatcct tgctcgcctg ctgctgaatc ctggcgacga ggttgttgtc
      721 gagacgcccg gctatcgtga cgcctttacc ctcttccagc tgcatggggc gcggctggtg
      781 gggctgcccg tcgacgagga ggggctgccc gtggatcgca ttcccgccga ctgccgggcg
      841 cgtctggcct tcgtgacgcc gacgcaccag tttcccaaag ggggcacgat gcccctggct
      901 cgccgcatgg ctttgttggc ctgggcgcgg gcgggcgggg cgctggtcat cgaagatgac
      961 tacgacggcg atctgcgcta cgatggccac tcgctggcgg cgctgcaagg gttagacgcc
     1021 gccggcgcgg acactgatgg ccgggtggcc tatctgggta ccttctctaa agtcctcttt
     1081 ccggccctac ggttgggcta cctggtgctg ccgccgccgc tggtgacgcc ctttgtccgc
     1141 gccaaagacc ttgtcgaccg cggcgcgccg acgctgacgc aggcggccgt ggccgacttc
     1201 atcgccgagg gacacttcga gcgccacctg cgccgcctgc gccgcctgta tggccagcgc
     1261 cgggcggcgc tgggcgcggc gctaaccgcc cggctgggcg agcgagtgca cttctcgccc
     1321 ctggcggccg ggctgcacgt gatgcttttc ccggaggcga acgtcgatga ggcccggcta
     1381 gtgcgcgagg cggccgcgcg tggcgtccgc gtccatcccg gcgcgcccta ccatctggag
     1441 cgccccgcgc cgccgtcgat cctgctcggt ttcagcggtc tgagcgagac ggagatcgtc
     1501 gagggcgtgc gccggctggc cgaagcctgg ccggtggccg gagcggccta acgcggcgct
     1561 agaccagcca gctcccttcc agccgcaaca gccacgcttt tgtctccaga ccgccgcgcg
     1621 cgccatagcc gtggagcttg ccatctgtcc ccaaaacgcg gtggcagggg atgatgatag
     1681 ggatggggtt gttggcgttg gcctggccga cggcccgcac cgcccccggc cggcccatct
     1741 ctgcggcaat ggccccgtag ctgctcgtct ggccataggg cacggcgtag acgcgcgtca
     1801 gcacctcccg cgcgaagggc gtcagcgccg gccagggcag aggcaggtcg aacacccgcc
     1861 gctcgccgcg caaatactca ttcaactgcg ccagcgcggc gatgacgatc ggccccggcg
     1921 gagcggccag gtctggcgtt tcgccggtca ggcgggccgc gtcgtcggcc aggcgcgttg
     1981 ggtcgtccca caggctgaca gccagcaggg tgctatcggc ggccgcggcc cagatcgtgc
     2041 cgatgggggt attatccagt cgtcccagtg tcaccatggc tcactccttc aatctcggcc
     2101 tgtggcgcgg cggcaaagcg caggtgatag acggcaatgc cgaaggcgac ggccacattg
     2161 agtgacgact tgccaccgta catgggcagg ctgaagacgc cgtcgcacag ggccagcaac
     2221 ccgggatcga cgccggcccg ctcgttgccg acgacgagag ccaggggggc gtccggcggc
     2281 ccggcgtcgt agagcgacgg ttgcggtgag gctccggccg tccgctccag cgcccacaga
     2341 cagaagccct cgtctctcag ccgcgccg
//
LOCUS       DAOSMQ010000496         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_45212_length_2357_cov_4.231538, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000496 DAOSMQ010000000
VERSION     DAOSMQ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45212_length_2357_cov_4.231538"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PK829_13285"
     CDS             complement(<1..664)
                     /locus_tag="PK829_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU42233.1"
                     /translation="MTDPTLLTDMPPAAANTAFVRGACPHDCPDACGTIVEVLDGRAV
                     AFYGDPAHPITDGWVCGKVRPYLEHVYHPDRLTTPLRRVGPKGSGQFAPISWDEAIAE
                     IGDRWRDIIAAYGAEAILPYSYSGTLGLAQMTVCNGRFWNRLGASQLERAICGDAAQY
                     AVERTLGARWAVPYADTAHTRAALIWGHNPASTAPHFMPYLKDIQRRGGTVVVIDPKR
                     TRT"
     gene            991..1608
                     /locus_tag="PK829_13290"
     CDS             991..1608
                     /locus_tag="PK829_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42234.1"
                     /translation="MRAYPLPSMLLLLAGLLLVACGGAPKQPPASFTPLLTATPLPTS
                     DSQQPVEIPVPPDDPSTWNLYLNREYGFSFHYPSDRWTLATAEDGRQLLLTYGDTGIA
                     LRVKFARQDDAGADLQIFNGEVADLVPEGTMRFIGADVERSMLAYEGLVEWVFYNDAA
                     PIPRGELLFSLALLSSNDYNAGVPAVVPDDVQAEADRILETFSLD"
     gene            complement(1723..>2357)
                     /locus_tag="PK829_13295"
     CDS             complement(1723..>2357)
                     /locus_tag="PK829_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42235.1"
                     /translation="RLEPAVWRAVAIPLPPLPEVGDAAVAPEQAEDQPPAQLFCHFAE
                     GTPVELLPPQAAPQIFLTPPRNPESLKPAHEWAQANNIALLPTSTCTEELLALGLGAS
                     ETAVWRITSPKDGDTISGNVPIVGTASFDPAVVQFYKIELGIPNGTDVQWLTLGEVHN
                     TPVVNGTLEVLQAEALPPGTYYLRLIVVKDSNYVGEPHQIQIHVEPPPSP"
BASE COUNT          395 a          775 c          777 g          410 t
ORIGIN      
        1 ctgtgcgcgt gcgcttgggg tcaatgacca cgaccgtgcc gccgcgccgc tggatgtcct
       61 tcagataggg catgaaatgg ggggcagtgc tggccgggtt gtggccccag atgagggccg
      121 cgcgcgtgtg ggcggtgtcg gcatagggca cggcccagcg cgcccccagc gtccgctcga
      181 cggcatattg agcggcgtcg ccgcagatgg cccgctccag ctggcttgcg ccgaggcggt
      241 tccagaagcg gccgttgcag acggtcatct gcgccagccc cagcgtaccg ctgtagctat
      301 agggcagaat ggcctcggcg ccatacgcgg cgatgatgtc ccgccagcgg tcgccgatct
      361 cggcgatagc ctcatcccag gagatgggcg cgaactgccc gctccccttc ggccccacgc
      421 ggcgcaatgg cgtcgtcagc cggtcggggt ggtagacgtg ctccaggtag gggcgcacct
      481 tgccacagac ccagccgtcg gtgatgggat gggcggggtc gccatagaag gccacggcac
      541 ggccgtcgag cacttcgacg attgtcccgc aagcatcggg acagtcgtgg gggcaggcgc
      601 cgcggacaaa ggcagtgttc gcggccgcag ggggcatatc ggtcagcagt gttgggtcgg
      661 tcaaggatga gacctcacag tcggccgcgc gggcgattca aaggcgatta tggcggaaaa
      721 caagaagcgc gtcaatttga cgccttagcc taccagggtt tttcagtcgt cgccagttgc
      781 tcaatgacgc caacccctta agccagctta ccgcctctag cgtgccgcct gacactctgc
      841 ggcaggcggc gcacactagg actgatcacc ggcccgcgct attcctgtcc gcgtagatcc
      901 gccggttcta cacgatccgt atgtcttctc tggccggcga gcagggcaat tgggctataa
      961 tgacggcatc tggagatgta aacctaactg atgagagcct accccctgcc ttcaatgctc
     1021 cttttgctgg ccggcctgct attggttgcc tgcggcggcg ccccaaaaca gccgccagcc
     1081 tccttcacgc ccctgctgac ggccaccccg ttgccgacca gcgactccca gcagccggtc
     1141 gaaatccctg tgccgccgga cgatccttcc acctggaatc tctacctcaa ccgggagtac
     1201 ggcttttcct tccactaccc gtccgaccgc tggacgctcg ccaccgccga agacggccgg
     1261 caactactgc tgacctacgg cgacacgggc attgccctgc gtgtcaagtt cgcccgccaa
     1321 gacgacgccg gcgccgacct gcaaatcttc aacggcgagg tggccgacct cgtgccggag
     1381 gggacaatgc gcttcatcgg cgcggacgtg gagcggtcga tgctggccta tgaggggctg
     1441 gtcgagtggg tcttctacaa cgacgccgcg cccatccccc gcggcgagct gctgttcagc
     1501 ctggcgctgc tgagcagcaa cgactacaac gccggcgttc cggccgtggt gccagacgat
     1561 gtgcaggcgg aggcggatcg cattctagag acgtttagcc tcgattgagt cgggcgtccc
     1621 cctccggccg gcagtggcgc gtgggcagga taccggctac agcgcaggac tattcgcgaa
     1681 cggccgcgcc agcttcgcaa taggagcgcg aagctggcaa gttcatggcg atggcggcgg
     1741 ctcgacgtga atctgtatct ggtgcggctc gccgacgtag ttgctatcct tgacaacgat
     1801 gaggcgtaga tagtaggtcc ccggtggcag cgcctcggcc tgcaacactt ccagcgtgcc
     1861 gttgaccacg ggcgtgttgt ggacctcgcc cagcgtcagc cattgcacat ctgtgccgtt
     1921 gggaataccc aactcgatct tgtagaactg caccacagct gggtcaaagc tggccgtgcc
     1981 gacgatgggc acgttgccgc tgatcgtgtc gccgtccttg ggcgaagtga tgcgccagac
     2041 ggccgtctcg ctggccccca gccccaacgc caatagctcc tcggtgcaag tggaggtcgg
     2101 caacagggcg atgttgttgg cctgggccca ctcgtgagcc ggcttcaggc tctccgggtt
     2161 gcgcggcggc gtcaggaaga tctgtggcgc ggcctggggc ggcaacaact ccaccggcgt
     2221 accctcggcg aagtgacaga agagctgcgc cggcggctgg tcttcggcct gctccggcgc
     2281 gacggcggcg tcgcccacct caggcaacgg cggcaaggga atggccacgg cccgccacac
     2341 cgccggctcc agccgtt
//
LOCUS       DAOSMQ010000497         2346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_45489_length_2346_cov_3.978175, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000497 DAOSMQ010000000
VERSION     DAOSMQ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45489_length_2346_cov_3.978175"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PK829_13300"
     CDS             <1..623
                     /locus_tag="PK829_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42236.1"
                     /translation="SQAFFRTNALLVRSGFLLGGLLVLLAPEFVRLALGPRWLPMVPT
                     FQFMAIFTLLDPLRVTISSLFVAVGRPGQVVRVRALQVAALVAGLFALGPRWGITGVA
                     VVVDGVLLLGLTWLLGSARAHVDYSARRLFLAPLLALATGGAVALGGQWLVCRAGPAV
                     CSNDWLTGALKGLLFAALFAGTLWTLERQAAVEMLGQLRGLWQPRS"
     gene            605..1534
                     /locus_tag="PK829_13305"
     CDS             605..1534
                     /locus_tag="PK829_13305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42237.1"
                     /translation="MATAIMNVTVGITSYNQRDYLIAAIDSVLAQTYLPAEILIVDDA
                     STDDSPQVISAYAGRHPDIVRPLLLTRNGGVNAARNHILNAATGDYLAFLDGDDRWLP
                     GKLARAAARLDAADRPDAVFDNFVFTDAQGQPQFVWAARARPPEGPLLRQVITLDLPR
                     TTLFRSELVPTALWRSAGYYDPSFRIYGDWDMRIRQAQVVGRYGYVDAVLSEYRRHGA
                     GLSAQSVDSHLAAVDRVEAKYASLIAHMEADAGGIHAGLRRWRAKLLQRAAYNTAAGR
                     ARGCRRQALAYYRRSLAYAPALDARALWQIVKP"
     gene            1531..>2346
                     /locus_tag="PK829_13310"
     CDS             1531..>2346
                     /locus_tag="PK829_13310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU42238.1"
                     /translation="MMSPSLPERSGWPGPAESPRPALAAPPRISVITPSYNQGRYLEA
                     TIRSVLAQEYPNLEYIIVDGGSTDDSVRIIDRYAKHLAGWTSRRDNGPAQAINRGFAQ
                     ATGDILAWLNSDDLYTPGALWAAADALAAHPEAALAYGEGWYIDEAGAPIEPCRFVRR
                     RFDRRYLVNRDPILQPAAFWRRALWDAAGPLDESLRWVFDWEWFIRAHAHGPFLYLPR
                     DMARYRVQPAALTRTGGLPRQLEHGRITRRYGAWWHPNHVVQQTRRLDAAGQQA"
BASE COUNT          320 a          856 c          761 g          409 t
ORIGIN      
        1 tgtcgcaggc gttcttccgc accaacgctc tgctggtgcg cagcggcttc ctgctgggcg
       61 ggctgctggt gttgctggcc ccggagttcg tgcgcctggc gttaggcccg cgctggctgc
      121 cgatggtgcc caccttccag tttatggcca tcttcacgct gctcgatccg ctgcgcgtga
      181 ctatcagtag cctgttcgtc gctgtcggcc ggccgggaca ggtcgtccgc gtgcgcgcgc
      241 tgcaagtggc ggcgctggtg gccggactat tcgctctcgg cccgcgctgg ggcatcaccg
      301 gcgtagccgt cgtcgtcgat ggggtactgc tgctggggct gacctggctg ctgggcagcg
      361 ctcgcgccca tgtggactat tcggcgcggc gcttgttcct ggccccgctc ctggcgctgg
      421 cgacgggcgg cgcggtcgcg ctgggagggc aatggttggt ctgtcgcgcg gggccggctg
      481 tctgctccaa cgattggctg accggggcgc tgaaagggct gttgttcgcc gctctctttg
      541 cgggtaccct ctggacgctg gagcggcaag cggccgtgga gatgctcggc caattgcgcg
      601 gcctgtggca accgcgatca tgaacgtcac cgtcggcatt acttcctaca accagcgcga
      661 ctatttgatc gccgccatcg acagcgtgct ggcccaaacg tacctgccgg ccgagattct
      721 catcgtcgat gacgcctcaa ccgacgactc gccccaggtc atcagtgcct acgccggccg
      781 tcaccctgat atcgtccgcc cgctgctgct gacgcgcaac ggcggcgtca atgccgcccg
      841 caaccacatc ctcaacgcag ccacaggtga ctacctggcc tttctcgacg gcgacgaccg
      901 ctggctgccg ggaaaactgg cgcgcgcggc cgcgcggctc gatgccgccg accggcccga
      961 cgccgttttc gataacttcg tcttcaccga cgcccagggc cagccccaat tcgtctgggc
     1021 ggcgcgagcg cgtccgccgg aggggccgct gctgcgccag gtgatcacgc tcgacctgcc
     1081 gcgcacgact ctattccgct ccgagctagt gcccacggcg ctatggcgtt cggccggcta
     1141 ctacgatccg tcgttccgca tctacggcga ctgggacatg cgcatccggc aggcgcaagt
     1201 tgtcggccgc tatggctacg tcgatgcagt cctcagcgag taccgccgcc acggcgcggg
     1261 cctgtcggcc cagagcgttg acagccattt ggctgccgtg gacagggtgg aggccaaata
     1321 tgccagcctg atcgcccaca tggaagcaga cgcgggcggc atacacgctg gcctgcgccg
     1381 ttggcgggcc aaactgttgc agcgcgccgc ctataacact gccgccggac gcgctcgcgg
     1441 ctgccgccgc caggccctgg cctattaccg ccgtagcctg gcctacgcgc ccgcgctgga
     1501 cgcgcgcgcc ctgtggcaaa tcgtcaagcc atgatgtcgc cctctctgcc cgaacgttcc
     1561 ggctggcccg ggccggccga atcgcctcgc ccggcgctgg ccgccccacc gcgcatcagc
     1621 gtcatcaccc cgtcgtacaa tcagggccgc tacctggagg cgactatccg ctctgtcctg
     1681 gcccaggagt atcccaatct agagtacatc atcgtggacg gcggctcgac cgacgacagc
     1741 gtccgcatca ttgatcgcta tgcgaagcac ctggccggct ggacatcgcg acgcgacaac
     1801 ggcccagccc aggccattaa ccgcggcttc gcccaggcca ccggcgacat cctggcctgg
     1861 ctcaactccg atgacctcta cacgcccggc gcgctctggg ccgcggccga cgccctcgcc
     1921 gcgcacccgg aggcagcgct ggcctatggt gagggctggt acatcgacga ggccggcgcg
     1981 cccatcgaac cgtgccgctt cgtgcgccgc cgcttcgacc gccgctatct ggtcaatcgc
     2041 gaccccatct tacagccggc ggccttctgg cggcgggcgc tctgggatgc ggcggggccg
     2101 ttggatgagt ccttgcgctg ggtcttcgac tgggagtggt tcattcgcgc ccacgcccac
     2161 ggccccttcc tctatctgcc gcgcgacatg gctcgttatc gcgttcagcc cgcggcgctg
     2221 acgcgcaccg gcggcctgcc gcggcagctg gagcacggcc gtatcacgcg ccgctacggc
     2281 gcgtggtggc accccaatca cgtcgtccaa caaacgcgcc ggctggacgc cgccggccag
     2341 caggcc
//
LOCUS       DAOSMQ010000498         2341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_45614_length_2341_cov_4.748031, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000498 DAOSMQ010000000
VERSION     DAOSMQ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45614_length_2341_cov_4.748031"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PK829_13315"
     CDS             <1..647
                     /locus_tag="PK829_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42239.1"
                     /translation="SMYITLFLPLSLLLSIVTVAIGDRVGAWLGAEAAPGEPRQSTLR
                     FVLSLLLPLLAGVALGLLAVFGARQQANIVNPETILALPADQPALDWMAANLPAGARV
                     AVNAWQWLGATWAGSDGGAWIVPLTGRQTTTPPVDHIYDANLFAQVRAFNEAAAAITD
                     WSDPAAAEWLRGQGVSHVYAGQRGGPFDPAKLSRNPGLALLYQNEGAFLYAVKP"
     gene            781..1395
                     /locus_tag="PK829_13320"
     CDS             781..1395
                     /locus_tag="PK829_13320"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOU42240.1"
                     /translation="MAFELPPLPYPEDALEPHVDARTMSIHHDKHHAAYTNNLNSALE
                     GQPALAGKSIEDLLGNLDAVPEAIRTTVRNNGGGYANHNLFWEIMGPGAGGQPTGELA
                     AAIDDAFGSFAAFKEQFAKAATTRFGSGWAWLYVDNNGGLVVASAPNQDTPLMEGNTP
                     ILGLDVWEHAYYLNYQNRRPDYIAAWWNVVNWNAVAEKYAAARK"
     gene            1458..1868
                     /locus_tag="PK829_13325"
     CDS             1458..1868
                     /locus_tag="PK829_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOU42241.1"
                     /translation="MTHIITRLCLRDTACVEVCPVECMVLGKPEETWPWLYIDPDTCI
                     DCGACVPECPYEAIFPEEEVPFDYVAPAGVWIANTKELLPDGAPFTGVIDGHNVALLN
                     AKQLAGGEKLDLTEDIPANYAFYSEGPGYDALNM"
     gene            1981..>2341
                     /locus_tag="PK829_13330"
     CDS             1981..>2341
                     /locus_tag="PK829_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOU42242.1"
                     /translation="MSDYRIEKDSLGEVRVPSGAYWGAQTQRAIHNFPITGLRPYPAF
                     VWSMALIKRAAAEVNRDLSLFSNKEAGGRAISSAEMAGAIIQAADEVLAGQWADQFVV
                     DPIQAGAGTSHNMNINEV"
BASE COUNT          444 a          807 c          689 g          401 t
ORIGIN      
        1 acagcatgta catcaccctc ttcctgccgc tgagcctgct gctgagcatc gtcacagtgg
       61 ccattggcga cagggtgggc gcctggctgg gcgcggaggc tgcgccgggc gagccgcgcc
      121 agtccactct acgctttgtg ctgtcgctgc tgttgccgct gctggccggc gtcgccctgg
      181 gcttgctggc cgtcttcggc gcgcgacaac aggccaacat cgtcaacccg gagacgatcc
      241 tcgccctacc ggccgaccag cccgcgctcg actggatggc cgccaacctg ccggccggcg
      301 cccgcgtggc cgtcaacgcc tggcaatggc tgggcgcgac ctgggccggc agcgatggcg
      361 gcgcgtggat cgtcccgctg acggggcggc aaacgaccac gccgccggtc gatcacatct
      421 acgatgccaa cctcttcgcg caggtgcgcg ccttcaatga ggccgccgcg gcgatcactg
      481 attggagcga cccggccgcg gccgaatggc tgcgcggcca gggcgtcagc cacgtctacg
      541 ccggccagcg cggcggccca ttcgacccgg ccaagctgtc gcgcaacccc ggcctcgcgc
      601 tgctctacca gaacgaaggc gctttcctct atgccgtcaa accatagagg gcagagaaat
      661 agccctccct gggtagggta attgcccgtt tgacaacggc taactctgcg ctagaatcaa
      721 attagcttgc gggtctgcaa gccatttcca ccggctgtcc tctattccaa ggagtacgtt
      781 atggcgtttg aattaccccc gctaccctat cccgaagatg cgttggagcc tcacgtcgac
      841 gcgcgcacga tgagcatcca ccacgataag caccacgccg cctacaccaa caaccttaac
      901 tcggcgctgg aaggccagcc cgctctggcc ggcaagagta tcgaagactt gctcggcaac
      961 ctggacgctg ttcccgaagc catccgcacg accgtccgca acaatggcgg cggctatgca
     1021 aaccataatc tgttctggga aatcatgggc cccggcgctg gcggccagcc caccggcgaa
     1081 ctggccgcag ccatcgacga cgcctttggt agcttcgccg ctttcaagga gcagttcgcc
     1141 aaggccgcca ccacgcgctt tggctctggt tgggcctggc tctacgtgga caacaacggt
     1201 ggccttgtcg tcgcctccgc tcctaaccag gacaccccgc tcatggaagg caacacgccc
     1261 atcctcggcc tggatgtctg ggaacacgcc tactacctca actatcagaa ccgccggccg
     1321 gactacatcg ccgcctggtg gaacgtcgtc aactggaacg ctgtggccga gaaatacgcc
     1381 gcggcgagaa aatgatcccc gactcgcgcc ggacgcattg acggcccggg cgaaaaacgc
     1441 tttgtaagga gagatacatg acccacatca tcacccgact ctgcctgcgc gacaccgcct
     1501 gtgtcgaagt gtgccccgtc gaatgcatgg tcctcggcaa gcctgaagag acctggccct
     1561 ggctgtatat cgaccccgac acctgcatcg actgcggcgc gtgcgtgccg gagtgcccct
     1621 atgaggccat cttccccgaa gaggaagtgc ccttcgacta cgtggctccg gccggcgtct
     1681 ggatcgccaa caccaaggag ctgctgcccg acggcgcgcc cttcaccggc gtcatcgacg
     1741 gccacaacgt tgccttgctc aacgccaagc aactggctgg cggtgagaag ttagacctga
     1801 ctgaggacat tccggccaac tacgccttct actccgaagg ccccggctac gacgcgctca
     1861 acatgtaagt tctgtggtag gggcgggtct aagacccgcc cctacaggac gaaccttgac
     1921 cggattgact gacctgtgac cctggttggc caatccggtt tttgcatcgc ggaggaatcc
     1981 atgagcgact atcgcatcga aaaggactcg ctgggcgaag tgcgcgtgcc gtccggagcc
     2041 tactggggcg cgcagacgca acgcgccatc cataacttcc ccatcaccgg cctgcgcccc
     2101 taccccgctt tcgtgtggtc gatggctctc atcaagcgcg ccgcggccga agtcaaccgt
     2161 gacctgagcc tttttagcaa caaagaggcc ggcggtcgcg ccatcagcag cgcggagatg
     2221 gctggagcca tcatccaggc cgccgacgaa gtgctggccg ggcaatgggc cgaccagttt
     2281 gtcgtcgatc ccattcaggc cggggccggc accagccaca atatgaacat caatgaggtc
     2341 a
//
LOCUS       DAOSMQ010000499         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_45794_length_2334_cov_4.283458, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000499 DAOSMQ010000000
VERSION     DAOSMQ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45794_length_2334_cov_4.283458"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK829_13335"
     CDS             complement(<1..204)
                     /locus_tag="PK829_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42243.1"
                     /translation="MQEITVSTPQGKGEDIARLAIACGLSGVTIARVRAYKGDQVSEE
                     EEVKVCTSAPLSTRFVEAVMAAPD"
     gene            431..>2334
                     /locus_tag="PK829_13340"
     CDS             431..>2334
                     /locus_tag="PK829_13340"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOU42244.1"
                     /translation="MPAPAGARFFLAVVGGAGWLVGIAVASAVDAGPGFWLLMAAAAL
                     AGAAALWRVGRVGLLLAGAAALALGGARLAAAQPSIDAGALAFYNGQTGVEVRGQVIA
                     EPERRDTSLRLRVAARAVVLDGRTRPVAGVVTVQTHRYPAVDYGATVSLRGDLEPLDA
                     LGSAGFAAYLERQGVASVMAYPDVTVLATDGGSPLRRALLAIRARGRITLAALLPEPQ
                     ASLLTGILLGDDSGLPRTTADAFQATGMTHVIAISGFNIALIVALLDRLTAPLLPRRS
                     GALVIILFLAFYTLLVGAAPSVVRAAIMGSAYLIGLRLLGRPTWALAGLFVAAFFMTL
                     LNPNTLWDVGFQLSFAATLGLMLYATPWLRRLDRGTEALLGPEAARPIRRLLGEAVVV
                     SLAAQVLVVPLLLYHFGRLSLASLPANLLVLPAQPAVMASGGAALFAGLLLPAAGRVV
                     AVPAWLFLTYTLAVIDRLASAPWASVPLRLSGPGLVAAYAVIGALTLLATRDRDTDRT
                     LAARWRLRPVPLVALGGAAVVVVLAVAWWAERPDGRLHVAFLDVGQGDAIFIQSPGGA
                     QVLIDGGRYPSVLLERLGEQMPFWDHSLDLVIATHPDDDHVAGLPEVVARYDVAQVIT
                     NGATADDAPA"
BASE COUNT          260 a          849 c          835 g          390 t
ORIGIN      
        1 gtcgggcgcg gccatgaccg cctcgacaaa gcgcgtggac aacggggccg acgtgcagac
       61 cttgacctct tcctcctcgc tgacctggtc gcccttgtag gcgcggacgc gcgcgatggt
      121 gacgccgctc agcccgcacg caatggccag acgcgcaatg tcttcgccct tgccctgagg
      181 agttgagacc gtgatttctt gcatactgtc ctatacccgc caacatactt tcccaggcgt
      241 agatagcata ggaaaaggaa taccctgtaa caatgtatcg ctggtataat tccgctgtga
      301 cctctgctgc gcggcggcca gcgcggccgg cccgccgtcc agaggccacg ccgagccagc
      361 aaccatgccg ccccgcgctc tcgcccccgc ccccgcgcca accgcctctc cggcggcaac
      421 gatccggagt gtgcccgcgc cggctggcgc gcgcttcttc ctcgccgtgg ttggcggcgc
      481 gggctggctg gtgggtatcg ccgtggcctc ggccgtggac gcagggccgg gcttctggct
      541 gctgatggcg gcggcggccc ttgccggcgc ggcggcgctg tggcgcgttg gccgcgtggg
      601 gctgctgctg gcgggcgcgg cagcgctggc cctgggcggg gcgcgtctgg ccgcggccca
      661 gccgtccata gacgcgggcg cgctggcgtt ttacaacggc cagactggcg tagaggtgcg
      721 cggccaggtc atcgccgagc cggagcggcg cgacaccagt ctgcgcctgc gcgtcgccgc
      781 gcgggcggtg gtcctcgacg gccggacgcg gccggtggcc ggcgtcgtca ctgtccagac
      841 ccatcgctat ccggccgtgg actatggcgc gaccgtgagt ctgcgcggcg atctggagcc
      901 gctggacgct ctgggcagcg ccggcttcgc cgcctacctg gagcggcagg gcgtcgccag
      961 cgtcatggcg tatccagacg tgaccgtgct ggccacggac ggcggctcgc ctctccggcg
     1021 cgccctcctg gccattcgcg cccgcggccg gataaccctg gcggcgctcc tccccgagcc
     1081 acaggcgtcg ctgctgaccg gcattctcct gggcgacgac agcggcctgc cgcggacgac
     1141 ggccgatgcc ttccaggcca ccggcatgac ccacgtcatc gccatctccg gcttcaacat
     1201 cgccctcatc gtcgccctgc tcgaccgcct gaccgcgccg ctgctgcccc ggcggtcggg
     1261 ggcgctggtc atcatcctct ttctggcctt ctacacgctg ctggtcggcg cggccccgtc
     1321 ggtggtgcgg gcggcgatta tgggcagcgc ctatctcatc ggcctgcggc tgctcggccg
     1381 gccaacgtgg gcgctggccg ggctgttcgt ggctgctttc tttatgacgc tgctcaaccc
     1441 caataccctg tgggatgtcg gcttccaact cagcttcgcg gcgacgctgg gcctgatgct
     1501 ctacgccacg ccctggttgc ggcggctgga tcgcggcacg gaagcgctgc tggggccgga
     1561 agcggcgcgg cccatccggc ggctgctggg cgaagcggtt gtcgtcagtc tggcggcgca
     1621 ggtgctggtc gtgccgctgc tgctctacca cttcggccgg ctgtcgctgg ccagcctacc
     1681 ggccaacttg ctggtgctgc cggcgcaacc ggcggtcatg gccagcggcg gcgcggccct
     1741 gttcgccggg ctgttgctgc cggccgccgg ccgcgtcgtc gccgtgccgg cgtggctctt
     1801 cctgacctac acgctggcgg tcatcgaccg gctggcgtcg gccccgtggg cgtccgtgcc
     1861 cctgcggctg tcgggaccgg ggctggtggc ggcctatgcc gtcatcggcg cgctgacgct
     1921 gctggccacg cgcgacaggg atacggatcg cacgctggcc gcgcggtggc ggctgcggcc
     1981 cgtgccgctg gtggcgctgg gcggcgcggc cgtggtcgtc gtgctggccg tcgcctggtg
     2041 ggcggagcgg ccagacggcc gcctgcacgt ggccttcctc gacgtgggcc agggcgacgc
     2101 catcttcatc cagtcgcctg gcggggcgca agtgctcatc gacggtgggc gctaccccag
     2161 cgtcctgctg gagcggctgg gcgagcagat gcctttctgg gaccactccc tcgacctggt
     2221 catcgccacc cacccggacg atgaccacgt cgccgggctg ccggaggtgg tggcccgcta
     2281 cgacgtagcc caggtaatca ccaacggggc cacggccgac gacgcgcccg ccta
//
LOCUS       DAOSMQ010000500         2329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_45932_length_2329_cov_4.528144, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000500 DAOSMQ010000000
VERSION     DAOSMQ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45932_length_2329_cov_4.528144"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PK829_13345"
     CDS             complement(<1..710)
                     /locus_tag="PK829_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU42245.1"
                     /translation="MVEQVPENLESTGPQLILIVDDELRMRRFIRMNMELEGYQVIEA
                     ENGIHALDQIRQFTPDLVVMDVMMPEMDGFETLKLLREISTVPVILLTVRDEEEDITR
                     GLALGADDYVTKPFSPRVLTSRVAAVLRRAHWPAPPPRTVLRIDDRLSVDFNRHQVIV
                     NGERIDLRPTEYRLLNHLIQNAGWVVPHDTLLAKVWGYEYREETHYLRLYINYLRKKI
                     EADPANPHYILTERGFGYR"
     gene            complement(718..960)
                     /locus_tag="PK829_13350"
     CDS             complement(718..960)
                     /locus_tag="PK829_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42246.1"
                     /translation="MRLSAIIQMLEEDSILGELDGPPNPAAHFITVHNPRRRDGRAVT
                     VFEANVETVLIAWHRVGVVQLLPQAEVEKAIGFVRE"
     gene            complement(979..1500)
                     /locus_tag="PK829_13355"
     CDS             complement(979..1500)
                     /locus_tag="PK829_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU42247.1"
                     /translation="MIACPECGAQHRPGTLFCDHCGAAIHPAAQAHVARTARPQPPAA
                     ADDAPQPLRRRPDTLPPTGDAQPELRVAIPHRRRDLSIRAAVIQIGRADPDAGSRPEL
                     DLTPFDGLERGVSRRHATIQWAEGGFVLIDQHSSNGTWLEGVRLVAGYAYQLPPRATV
                     RFGDLVVQLSTSD"
     gene            complement(1497..>2329)
                     /locus_tag="PK829_13360"
     CDS             complement(1497..>2329)
                     /locus_tag="PK829_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42248.1"
                     /translation="RVAHLILLTDGHTYGDADHCLRLAREAAARGVGITAFGIGSDWN
                     DAFLDALVAPSGGQSHYAAAPGDILPYLEERLAGLGDIYAREVRLLAGWPGQLSLRAG
                     FKLAPFPQPLALDTNPIPLGDLEGRAPMTLLLEFIVAPQAMAARFRLPIEIHYRRPGG
                     GEASLSRSTQLLVQAGAADDAPPSPALIEAARRLNLYRMQERAWEEAQSGQLDTAAAR
                     MRRLTARYLETGDLRLAQQAQQEAQRLGRQSTMSLEGRKVLKYGTRSLLGQLPREPES
                     "
BASE COUNT          375 a          726 c          848 g          380 t
ORIGIN      
        1 cgatagccga agccgcgctc ggtcaggatg tagtgcgggt tggccgggtc tgcctcgatc
       61 ttcttgcgca ggtagttgat gtagaggcgg agatagtggg tttcttcgcg gtactcatag
      121 ccccagactt tggccagcag ggtgtcgtgg ggaacgaccc agccggcgtt ctggatgagg
      181 tggttcagca ggcggtactc ggtcggccgc aggtcaatgc gctcgccgtt gacgatgacc
      241 tggtggcggt tgaagtccac cgacaggcgg tcgtcaatgc gcaggacggt gcggggcggc
      301 ggcgcgggcc agtgggcgcg ccgcagcacc gcggccacac gactggtcag cacgcgcggg
      361 ctgaagggct tggtcacgta gtcatcggcc cccagggcca ggccgcgggt aatgtcttcc
      421 tcttcgtcgc gaacggtcag cagaatgacg ggcacggtgg agatttcgcg cagcaacttc
      481 agcgtctcga agccgtccat ctccggcatc atcacgtcca tgaccaccag gtcaggcgtg
      541 aactggcgga tctggtcgag agcgtggatg ccattctcgg cctcaatgac ctgatagccc
      601 tccaactcca tgttcatgcg gatgaagcgg cgcatgcgca actcgtcatc gacgatgagg
      661 atgagttgtg ggccggtgga ctccagattc tcgggaactt gctcgaccat agggcactca
      721 ctcgcggaca aagccgatgg ccttctccac ttcggcctgg ggtagcaact gaacgacgcc
      781 gacgcgatgc caggcgatga ggacggtctc aacgttggcc tcaaagacgg tcacggcgcg
      841 gccatcgcga cggcgggggt tatgaacggt gatgaagtgc gccgccgggt taggcgggcc
      901 gtccagttcg cccaggatgg agtcttcttc caacatctga atgatcgccg acagtctcat
      961 aggcccttgc tctccagttt aatcgcttgt ggacagttgt accactaggt cgccaaagcg
     1021 cacggtggcg cgcggtggca gttggtaggc gtagccggcc accaggcgga cgccctctag
     1081 ccatgtgccg ttgctgctat gctggtcgat gagcacgaag ccgccctcag cccactggat
     1141 ggtcgcgtgg cggcgcgaga cgccgcgctc caggccgtcg aaaggggtca ggtcgagttc
     1201 ggggcgggag ccggcgtcgg ggtcggcccg gccgatctgg atgacggcgg cgcgaatgga
     1261 caggtcgcgg cggcggtggg gaatggcgac tcgcaactcc ggctgagcat cgccggttgg
     1321 cggcaatgta tcggggcgac gacggagggg ttggggagca tcgtcggccg cggccggcgg
     1381 ctgtggccgc gcggtgcggg ccacgtgagc ctgcgccgcc gggtggatgg ccgcgccgca
     1441 gtggtcgcag aacagcgtcc ccggccggtg ctgcgcgcca cactccgggc aggcgatcac
     1501 gattccggct cccgcggcaa ctgccccaac agcgagcgtg tgccgtactt caacaccttg
     1561 cgcccttcca gcgacatcgt gctctggcgg ccgagccgtt gggcctcctg ttgcgcctgt
     1621 tgcgccaggc gcaggtcgcc cgtctccagg tagcgggcgg tcagacgacg catccgcgcg
     1681 gcggcggtgt ccagttggcc cgactgggcc tcctcccagg cccgttcctg catccggtag
     1741 agattcagtc gccgggcggc ctcgatgagc gcgggcgatg gcggcgcgtc atcggccgcg
     1801 ccagcctgca cgagcaactg ggtcgagcgg ctgagcgacg cctcgccacc gccgggccgg
     1861 cgatagtgta tctcgatggg cagccggaag cgcgcagcca tcgcctgtgg ggcgacaatg
     1921 aactccagca gcagggtcat tggcgcgcgc ccttccaggt cacccagagg gatggggttg
     1981 gtgtccagcg ccagcggctg aggaaagggg gccagcttga agccagcgcg cagcgacaac
     2041 tggcccggcc agcccgccag caggcgcact tcgcgggcgt agatatcgcc cagcccggcc
     2101 agcctctcct ccaggtaggg caagatgtca ccaggggcgg cggcgtagtg ggattggccg
     2161 ccggacggag ccaccagcgc gtcgaggaaa gcgtcgttcc agtcggagcc gatgccgaag
     2221 gcggtgatgc cgacgccgcg cgccgcggcc tcacgagcca ggcgcagaca gtggtcggcg
     2281 tcgccgtacg tgtggccgtc ggtcagcaga atgaggtggg cgacgcgcc
//
LOCUS       DAOSMQ010000501         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_46043_length_2325_cov_4.649780, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000501 DAOSMQ010000000
VERSION     DAOSMQ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46043_length_2325_cov_4.649780"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK829_13365"
     CDS             <1..275
                     /locus_tag="PK829_13365"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOU42249.1"
                     /translation="DAPLKLYIIATAHERARRRLRDRRKLGFDDDFDAILADVIRRDH
                     IDSTREHSPLRPAADSTVIDTTGRSVADLIAQILTLLNHTPTEEPA"
     gene            275..1723
                     /locus_tag="PK829_13370"
     CDS             275..1723
                     /locus_tag="PK829_13370"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOU42250.1"
                     /translation="MPLFQEVDLTTFAGGRVVAIEPGSVAAAVGLRPGDELLAINDET
                     VEDVIDVQFHGAEEELELLVRREGEYLLFEAERDYDQELGLRFAHPTFDVDIRRCNNL
                     CPFCFVLQMAPRFRRTLYIKDDDYRYSFLFGHFVTLTNLSDHDWQRIERMRLSPLYVS
                     VHVTDTELRRQFLRNSTAPDIMAQLRWLAARHIEVHTQLVIVPGFNDGAWLERSIREL
                     AELWPGDGGGGVLSVSVVPVGLTRHHKYGMRPHTPAEAAATLAYVEALQPQFQQRFGL
                     RFVYPTDEWYLVSGREVPPLAAYDGQALHENGLGMVRAFLDDWAGVREEIAATVAKPP
                     PRALTLATATLFAPTLQPVAAEFAALTGCAVEVVPILNRRLGETITVAGLLMAGDVID
                     QLLAAGCGDLVVLPRVMFDHPDTIALDDLSPQDAANRLGRPVALADSLGDVWDALTGV
                     SRVLYRPGVVPGDSIDLRVLGDDAGESAAHLS"
     gene            complement(1730..>2325)
                     /locus_tag="PK829_13375"
     CDS             complement(1730..>2325)
                     /locus_tag="PK829_13375"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU42251.1"
                     /translation="AVDDSYTVALGETLTVAAPGVLANDANPGNAPLTAVLVEGPLSG
                     TLSLAADGSFTYTSTEEFAGQDSFTYRADNGLASNVATVTIAVFDPNGPPVAVDDSYI
                     ALVGQRLTVAAPGVLANDVNPQANGMTAVLAAGPAHGTLALRADGSFTYTPQAGYQGA
                     DQFTYRANNGQPSNVATVRITVSRSGFRVFLPVAVSD"
BASE COUNT          353 a          816 c          769 g          387 t
ORIGIN      
        1 ccgacgcgcc actgaagctc tatatcatcg ccacggccca cgagcgggcg cgccgccgcc
       61 tgcgcgaccg ccgcaagctg ggcttcgacg acgatttcga cgccatcctg gccgacgtca
      121 tccgccgcga ccatatcgac agcacccgcg agcactcgcc cctccgcccc gcggccgaca
      181 gcaccgtcat cgacacgacc ggccgcagtg tcgccgatct gatcgcgcaa attctgacgc
      241 tgctgaacca cacgccgacc gaggagcctg cctgatgccc ctttttcagg aagtagacct
      301 gacgaccttt gccggcgggc gcgtcgtcgc catcgagccg ggcagcgtgg ccgcggccgt
      361 tggcctgcgg ccgggcgacg agctgctggc catcaacgac gagacggtcg aagacgtcat
      421 cgacgtgcaa ttccacggcg cggaggagga gttggagttg ctggtgcgcc gcgagggcga
      481 gtatttgctc ttcgaggccg agcgcgacta tgaccaggag ttgggcctgc gcttcgccca
      541 cccgacgttc gacgtggata tccgccgctg caacaacctc tgcccgttct gcttcgtgct
      601 gcaaatggcc cccaggttcc gccggacact ctatatcaag gacgacgatt accgttactc
      661 cttccttttc ggccactttg ttaccctgac caacctgagc gaccacgact ggcagcgcat
      721 cgagcggatg cggctgtcgc cgctgtacgt ctccgtccac gtgaccgaca cagagttgcg
      781 ccgccaattt ctgcgcaact ccaccgcgcc agacatcatg gcgcaattgc gctggctggc
      841 ggcgcgccac atcgaggtgc atactcaact cgtcatcgtg cccggcttca acgacggcgc
      901 gtggctggag cgctcgatcc gcgagctggc tgagttgtgg ccaggggatg gcggcggtgg
      961 ggtgctgtca gtcagcgttg tgcccgttgg cctgacgcgc caccacaagt acggcatgcg
     1021 cccccacacc ccggccgagg ccgcggcgac gctcgcttac gtcgaagcgt tgcagcccca
     1081 gttccagcag cgcttcggtc tccgcttcgt ctacccgacc gacgagtggt atctcgttag
     1141 cgggcgcgaa gtgccgccac tggcagccta cgacggccag gccctgcacg agaacggcct
     1201 gggcatggtg cgcgcctttt tagacgattg ggccggcgtg cgcgaagaga tcgccgccac
     1261 cgtggcgaag ccgccccccc gcgccctcac cctggccaca gcgacgctct ttgcgccgac
     1321 gctgcaaccg gtggcggccg agttcgccgc cctgaccggt tgtgccgtgg aagtcgtgcc
     1381 catcctcaac cggcggctgg gcgagacgat caccgtcgcc gggctgctga tggccggcga
     1441 tgtcatcgac caactgctgg ccgcgggttg cggcgatctt gtcgtcctgc cgcgggtcat
     1501 gttcgaccat cccgacacca tcgctctcga cgacctgtcg ccgcaggacg cggccaaccg
     1561 cctgggccgg ccggtggccc tggccgactc gctgggcgat gtctgggacg cgctcaccgg
     1621 cgtctcgcgg gtgctgtacc ggccgggcgt tgtcccgggc gacagcatcg acctgcgcgt
     1681 cctcggcgac gacgccggtg aatcagccgc ccatttgtcc tgatccgcct cagtcggaca
     1741 ccgccacggg caagaaaacc cgaaagccgc tgcgcgacac cgtgatcctc actgtggcca
     1801 cgttcgacgg ctggccgttg ttggcccgat aggtaaactg gtccgcgccc tgatagccgg
     1861 cttgcggcgt gtaggtgaac gagccgtcgg cgcgcaacgc cagtgtgccg tgggccgggc
     1921 cggcggccag gacggcggtc atgccgttcg cctgagggtt gacgtcgttg gccagcacgc
     1981 ccggcgcggc gacggtcagg cgctggccca ccagggcgat gtagctatcg tccacggcca
     2041 ccggcgggcc gttgggatcg aagacggcga tggtgacggt ggcgacgttg gaggccaggc
     2101 cgttgtcggc ccggtaggtg aagctgtcct gcccggcaaa ctcctcggtg gaggtatagg
     2161 tgaagctgcc atcggcggcc aggctcaacg taccgctcag cggcccctcg acaagcacgg
     2221 ccgtcagcgg cgcgttgcca gggttggcgt cattggccag cacgcccggc gcggcgacgg
     2281 tcagcgtctc tcccagggcg acggtgtagc tgtcgtccac ggccg
//
LOCUS       DAOSMQ010000502         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_46130_length_2322_cov_3.064402, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000502 DAOSMQ010000000
VERSION     DAOSMQ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46130_length_2322_cov_3.064402"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2322
                     /gene="dnaE"
                     /locus_tag="PK829_13380"
     CDS             <1..>2322
                     /gene="dnaE"
                     /locus_tag="PK829_13380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU42252.1"
                     /translation="PGHLVLLAAEPAGYRSLCRLASLFQADADRERRRRAGLTWAELR
                     ANADGLVCLTGGRACWLERFLRAGNRAAATRYVAQLGGVFGEGCCLAVSPDLMAGPGR
                     PLAEEIIALGSRFGLRVVAAQPIYCLDAQQRPLLRLLAAIDANCPVAEVDPSALPDCG
                     DPASALEWPAPEAFAAAYADRPELLAAAGDVADGCGEALPDGRLLWPVLAGDMPAPVQ
                     LHRDAAAGLAERYGAALPADALARLEREIAAVVDYGYAPLFLLVADLVRFARSAAIPV
                     STRGSVANSLLAYCLGITTVDPIAHDLLFERFLNPARRSPPDIDLDFCSRRRDEVLDY
                     VRQTYGDDRVALVSTMTTFQPRSAVREAGKAHALPDETIDALVAILPHGWHPDPRRRS
                     TETPADVLAKIADPTQREVVRLAYLLVSRPHYPGLHPGGLVITPGPLTDTVPVLLSPK
                     GFLATQYEHGDVETIGLPKLDLLGIRALTVLADAVELVRHFHAPAFRLEDIPPGDAAT
                     GDMLSRGDSIGVFQCESDGARRTLRQLRARNVGDLAIAGAFFKPGPALGGMARAFVAR
                     YRGQEPVTYLHPALEPILGRTQGVLLFQEQILRIAVEIAGLSWAQADGLRRGISRFRA
                     NELEALAAAFADGCCRPAPDGPGFSREQAARLWEQIVPFAGYGFNQGHATAYADVSYR
                     SAYVKAHWPAEFLCARLADAGGFHHPAVYMAEAVRLGVAVRPPHVNFSGHSFTLTYEH
                     APDSEQPILWMGLGQVRDLRAASVAAIRAARAGA"
BASE COUNT          277 a          926 c          761 g          358 t
ORIGIN      
        1 cccggccacc tggtgctgct ggcggccgag ccggccggct accgctctct gtgccgtctg
       61 gcatcgctct ttcaggccga cgccgaccgc gagcgccgcc gccgcgccgg cctgacgtgg
      121 gccgagttac gcgccaatgc cgacggattg gtctgcctga ctggtggccg cgcctgctgg
      181 ctagagcgct tcctgcgggc cggcaaccgc gccgcggcga cgcgctacgt ggcccagctc
      241 ggcggcgtct tcggcgaagg gtgctgcctg gcggtcagcc ccgacctgat ggccgggccg
      301 ggccggccgc tggccgagga gatcatcgct ctgggcagcc gctttggcct gcgcgttgtc
      361 gccgcccagc ccatttattg cctcgacgcc cagcagcggc cgctcctacg tctgctggcg
      421 gccatcgacg ccaattgccc cgtggccgag gtagacccca gcgccctgcc cgactgtggc
      481 gacccggcca gcgccctgga gtggcccgcg ccggaggcct tcgcggccgc ctacgccgac
      541 cgcccggaac tgctggccgc cgccggcgac gtggccgacg gctgcggcga ggcgctgccc
      601 gacggccgcc tgctctggcc cgtcctggcc ggcgacatgc ccgcgcctgt ccaactgcac
      661 cgcgacgccg cggccgggct ggccgagcgc tatggcgcgg ctctgcccgc ggacgccctg
      721 gcccgcctgg agcgagagat tgccgccgtg gtcgactacg gctatgcgcc cctctttctg
      781 ctggtggccg acctggtgcg cttcgcccgt tccgcggcca tccccgtcag cacgcgcggc
      841 agcgtggcca actcgctgct ggcctattgc ctgggcatca ccaccgtcga tcccatcgcc
      901 cacgatctgc tcttcgagcg ctttctgaac cccgcccgcc gcagtccgcc cgacatcgac
      961 ctggatttct gtagccggcg gcgcgacgag gtgctcgatt acgtccgcca gacctacggc
     1021 gacgaccgcg ttgccctggt cagcaccatg accaccttcc agccacgctc agccgtgcgc
     1081 gaggccggca aggcccacgc cctgcccgat gagaccattg acgcgctggt ggccattctg
     1141 ccccacggct ggcatcccga cccgcgccgc cgctcgacgg agacgccggc ggacgtgctg
     1201 gccaaaatcg ccgacccgac ccagcgcgag gtcgtccgtc tggcctatct gctcgtcagc
     1261 cggccccact atcccggcct gcaccccggc ggtctggtca tcacgcccgg cccgctgacc
     1321 gacaccgtgc ccgtcctgct ctcgcccaag ggcttcctgg cgacccagta cgagcatggc
     1381 gacgttgaga ccattggcct gcccaagctc gacctcctgg gcatccgtgc cctgaccgtg
     1441 ctggccgatg ccgtcgaact ggtgcgccac tttcatgcgc cggccttccg cctggaggac
     1501 atcccgcccg gcgacgcggc cacgggcgat atgctcagcc gcggcgactc cattggcgtt
     1561 ttccagtgcg agagtgacgg cgcgcggcgc accctgcgcc agttgcgcgc ccgcaacgtg
     1621 ggtgacctgg ccattgccgg cgcgttcttc aagcccggcc cggccctggg cggcatggcc
     1681 cgcgccttcg ttgcccgcta tcggggccag gagccagtca cctacctcca cccggcgctg
     1741 gagccgatcc tcggccgcac ccagggcgtg ctgctcttcc aggagcaaat cctgcgcatc
     1801 gccgtggaga tcgccgggct gagctgggcg caggccgacg gcctgcggcg cgggatcagc
     1861 cgcttccggg ccaacgagtt ggaagcgttg gccgcagcct ttgccgatgg ctgctgccgc
     1921 cccgcgcccg acggccccgg cttcagtcgt gaacaggcgg cccgcctctg ggagcagatt
     1981 gtccccttcg ccggctacgg tttcaaccag ggccacgcca cggcctacgc cgacgtcagc
     2041 taccgctccg cctacgtcaa ggcccactgg ccggctgagt tcctctgcgc ccgcctggcc
     2101 gacgcgggcg gcttccacca cccggccgtc tacatggccg aggccgtccg gttgggcgtt
     2161 gccgtgcgcc cgccccatgt caacttcagc ggccacagct tcacgctgac ctacgagcac
     2221 gcccccgaca gcgagcagcc catcttgtgg atgggcctcg gccaggtgcg cgacctgcgc
     2281 gccgcgtccg tcgccgccat ccgcgcggcg cgggcgggcg cg
//
LOCUS       DAOSMQ010000503         2314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_46342_length_2314_cov_4.748119, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000503 DAOSMQ010000000
VERSION     DAOSMQ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46342_length_2314_cov_4.748119"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1035
                     /locus_tag="PK829_13385"
     CDS             73..1035
                     /locus_tag="PK829_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU42253.1"
                     /translation="MRIGVFTALFQQFPLEVALDKAVAAGVTAVEIGAGGYPGSQHCR
                     PADLLDDERARAAFQEAIAARGLLLSALSVHNNPLHPDPAVAQTADAELRAAIRLAEA
                     LGVPVVNGFAGLPAGAPGDTSPNWVTCPWPPDFLRMLDYQWNEVAIPYWRELETFAAG
                     HNVRVAFEMHPCMLVYNVETLLRLRAATGPALGCNFDPSHLWWNGVDPVEAIRALGPA
                     IFHVHGKDCALDAHNIRVNGCNDHKAYDRILERAWTFRSIGYGHDAAVWRAIVSMLRL
                     VGYDYVISIEHEDALMSTDEGLAKGIALLQEVVMAAAPGEMFWA"
     gene            1128..1388
                     /locus_tag="PK829_13390"
     CDS             1128..1388
                     /locus_tag="PK829_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HOU42254.1"
                     /translation="MGTSATTTIRISRRTRDILQALAEQYDSSMQAVLEEAVDLLEGQ
                     RLLEATNEAYAALRANPEAWQELQEERAEWDATLSDGLDGLL"
     gene            1388..1732
                     /locus_tag="PK829_13395"
     CDS             1388..1732
                     /locus_tag="PK829_13395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOU42255.1"
                     /translation="MPSPSRGEIWWANLDPTKGREQGGRRPVLIVSTTYFNTGPADLV
                     FVVPLTRTDRGVPIHVPIQPPEGGVMAPSLILCDALRSISKERLGDRPLGSVSTKTMQ
                     RVESILRLLMKL"
     gene            complement(1744..>2314)
                     /locus_tag="PK829_13400"
     CDS             complement(1744..>2314)
                     /locus_tag="PK829_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42256.1"
                     /translation="TAASLIGDPFLLRTVGGRPLRLSPGVAAPHSAAAAEMLAAAVLD
                     AVALAPGETVLESRAGPGWLTAALAARAAEVIAIEPNPDAVADAAENLDAFDNVSLYE
                     ATEAEALPALAQTPAVAVFHGAGELPAAALDWLAQARPERLVVVAEVGLLAKEAKRLA
                     GAGYRPVAFQPVDVGPQGFEVEVVGGWAR"
BASE COUNT          409 a          823 c          701 g          381 t
ORIGIN      
        1 tttactgttc actgacctac tgaccactga cccactgacc actgaccgct aaccactaaa
       61 aaggagtccc ccatgcgcat cggtgtcttc accgccctgt tccaacaatt ccccctcgag
      121 gtcgccctcg acaaggccgt cgccgccggc gtgaccgccg tggagattgg cgccggcggc
      181 taccccggca gccagcactg ccgcccggcc gacctgctcg acgatgagcg cgcccgcgcc
      241 gccttccagg aggccatcgc cgcgcgcggg ctgctgctca gcgctctcag cgtccacaac
      301 aacccgctgc accccgaccc ggccgtggcc cagacggccg acgccgagtt gcgcgccgcc
      361 atccgcctgg ccgaggccct gggcgtgccc gtggtcaacg gcttcgccgg tctgccggcc
      421 ggcgcgcccg gcgacacatc gcccaactgg gtcacttgcc cctggccgcc cgacttcctg
      481 cgcatgctcg actaccagtg gaacgaggtc gccattccct actggcgcga gttggagacg
      541 ttcgccgccg gccacaacgt ccgggtcgcc ttcgagatgc acccctgcat gctggtctac
      601 aacgtcgaga cgctgctgcg gctgcgcgcg gccaccggcc cggccctagg ctgcaacttc
      661 gaccccagcc acctgtggtg gaacggcgtc gatccggtcg aagccatccg cgccctcggc
      721 ccggccatct tccacgtcca cggcaaagac tgcgccctgg acgcccacaa catccgcgtc
      781 aatggctgca acgaccacaa ggcctatgac cgcatcctgg agcgggcgtg gacgttccgc
      841 agcatcggct acggccacga cgccgccgtc tggcgcgcca tcgtcagcat gttgcggctg
      901 gtcggctacg actacgtcat ctccatcgag cacgaggacg cgctgatgtc caccgacgaa
      961 gggctggcca agggcatcgc cctgctgcaa gaggtcgtta tggccgccgc gccgggggag
     1021 atgttttggg cgtaagaaaa gagatctccg cctgcccgca tgcgttgaca aaaacgctct
     1081 ctcctatgta tactcaattg tatactaaat tgtggagcaa tgaaatcatg gggacgagcg
     1141 caacaacaac gataagaata tcacggcgaa ctcgcgacat cctccaagcc cttgctgagc
     1201 agtatgatag ctccatgcag gccgttctgg aggaagccgt ggatctgtta gaaggtcagc
     1261 ggctcctgga ggcaactaac gaagcctatg cggcattgcg ggccaaccct gaggcgtggc
     1321 aagagcttca ggaggaacga gccgagtggg atgcaacctt aagcgatgga ctggatggtc
     1381 tactgtgatg ccttcaccca gccgaggcga gatctggtgg gccaatcttg accccacaaa
     1441 aggtcgtgaa cagggtggac ggcggccagt actgattgtc tccacgacct atttcaatac
     1501 cggcccagcc gacctcgttt ttgttgttcc cctcactcgc actgatcgcg gagtccctat
     1561 tcatgtacca attcaaccac cggagggtgg cgtgatggcc cccagcttaa tactgtgcga
     1621 cgcgctcagg tcgatttcta aggagagatt aggtgatcgc cccttgggaa gtgtatcgac
     1681 taagacgatg caacgagtgg agagtatctt gcgattgctg atgaagctgt agttgctttg
     1741 ggtttagcgc gcccatccgc ccaccacctc cacctcaaac ccctgcggcc ccacatccac
     1801 cggctggaat gccaccggcc ggtagcccgc ccccgccagc cgcttggcct ccttggccag
     1861 cagcccgacc tcagcgacga cgaccagccg ctccggccgc gcctgtgcca gccagtccag
     1921 cgccgccgcc ggcaactcgc ccgcgccgtg gaagacggcc acggctggcg tctgcgccag
     1981 cgccggcagc gcttccgcct ccgtggcctc gtagagcgac acgttgtcga aggcgtcgag
     2041 gttctcggcc gcgtcggcca cggcgtcggg attcggctcg atggcgatga cctcggccgc
     2101 gcgggccgcc aatgccgctg tcagccagcc ggggccggcg cggctctcca gcaccgtctc
     2161 gcccggggcg agtgccaccg cgtcgagcac ggccgcggcc agcatctccg ccgcggcggc
     2221 gctgtggggc gcggccacgc cgggcgagag gcgcaacggc cgcccgccaa ccgtccgcag
     2281 caggaacggg tcgccgatca gcgacgccgc cgtg
//
LOCUS       DAOSMQ010000504         2290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_47019_length_2290_cov_3.769575, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000504 DAOSMQ010000000
VERSION     DAOSMQ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47019_length_2290_cov_3.769575"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PK829_13405"
     CDS             <1..590
                     /locus_tag="PK829_13405"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOU42257.1"
                     /translation="KGTRARMGKGENVWTAFTTLMVRNRRRYGGYWIHMGVLIMAFGI
                     VADGLYQSETQIRLTAGESVQVGDFEMVFNSVSRFPGADDLLVTEADVDVYRDGEFLR
                     KLTPQIEFFQRSGQPMTIPSARSTITEDFYVILVNWEPTTADAATFRIFLNPLINWIW
                     AGGVIFILGTLIAAWPDREKAPARARKPAAVVVGD"
     gene            637..1236
                     /locus_tag="PK829_13410"
     CDS             637..1236
                     /locus_tag="PK829_13410"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOU42258.1"
                     /translation="MTRYYRLVRWLVLALLLALLPAVALAQDDGTITDDEVNEVAKDL
                     YCPVCESTPLDVCPTQACADWRELIRTKLAAGETRQDVLDYFARQYGDGVLADPPRHG
                     ASLVVLWVLPVLVVLLGALLFARLLRSMRATGPAATVAGMTGTVTTGTATTSTVATGM
                     ATTGMATTGTAASPPRADERRGDEALDDYIARVERELKE"
     gene            1262..1858
                     /locus_tag="PK829_13415"
     CDS             1262..1858
                     /locus_tag="PK829_13415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU42259.1"
                     /translation="MSETIPTPTTPPAAEPAKARRPLRLSIILLWVGVIALIALLGWG
                     LLNSTATRPEVGQQAPDFDVEFFDGYDWDGRTTANLADMRGQVVVLNFWASWCVECRD
                     ETPMLEAAWQRYAGQGVVVLGVAYADVEPNSLAYMKEFGVSFPHAPDLGTDISQTYEI
                     TGVPETFIIDKDGIIRHVQIGAVNTAMLDQIIPQLLAQ"
     gene            1879..>2290
                     /locus_tag="PK829_13420"
     CDS             1879..>2290
                     /locus_tag="PK829_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42260.1"
                     /translation="MIAGITLGSLLLGVALVVVVVLYLARPFVTAEDEDVRLAREEAD
                     SLALRRDALLRQVRELDDDMEAAKVAPELYQRVRPQLVKQAAIIMQQLDARQADALTP
                     DLDAQIEAAVRRLRTPDEIDADIEAAVRAARGRPA"
BASE COUNT          385 a          777 c          770 g          358 t
ORIGIN      
        1 ccaagggcac gcgggcgcgc atgggcaagg gcgagaacgt ctggacggcc ttcacgacgc
       61 tgatggtccg caatcggcgg cgctacggcg gctactggat ccacatgggt gtgctcatca
      121 tggccttcgg catcgtcgcc gatggcctct atcagagcga gacgcagatt cgcctgacgg
      181 ccggtgagag tgtccaggtg ggtgactttg aaatggtctt caacagcgtc agccgctttc
      241 cgggggccga cgacctgctg gtcaccgaag ccgacgtgga tgtctatcgg gacggcgagt
      301 tcctgcgcaa actgacgccg cagatcgagt tcttccagcg ctccggccag ccgatgacta
      361 tcccctcggc ccgctcgacg attaccgagg acttctacgt catcctggtc aactgggagc
      421 cgacgacggc cgacgcggcc acgttccgca tcttcctgaa cccgctcatc aactggatct
      481 gggcaggcgg cgtcatcttc attctcggca cgctcatcgc cgcctggccc gaccgggaga
      541 aggctcctgc tcgcgcccgc aagccggccg ccgtggtcgt gggcgactag gccggggacg
      601 catcgtcgcc acggcgcgca gtagaaacaa acgacaatga cgcgatatta ccgactcgtt
      661 cgctggctcg tcctggccct gctgctggcc ctgttgccgg ccgtggccct ggcccaggac
      721 gacggcacga tcaccgacga cgaggtgaac gaggtcgcca aagacctcta ctgcccggtg
      781 tgcgagagca cgccgctgga tgtctgcccg acgcaggcct gcgccgactg gcgcgaactg
      841 atccgcacca agctggccgc gggcgagacg cggcaggacg tgctcgacta cttcgcccgg
      901 caatatggcg atggcgtcct ggccgacccg ccccgccacg gggccagtct ggtggttcta
      961 tgggtcttgc cggtgctggt cgtcttgctg ggggcgctgc tgtttgcccg gctgctgcgc
     1021 agcatgcgcg cgaccgggcc ggcggcaacc gtcgccggca tgaccggcac ggtcacgacc
     1081 ggcacggcca cgaccagcac ggtcgcgacc ggcatggcca cgaccggcat ggccacgacc
     1141 ggcacggccg cgtcgccgcc ccgcgccgat gagcgccgcg gcgacgaagc cctggatgac
     1201 tacatcgccc gcgtggagcg ggagctaaag gaatagcgac gggaagatag gggctagtca
     1261 catgagtgaa acaatcccaa cgccaacgac cccacccgcg gccgagccgg ccaaggcgcg
     1321 ccggccgctg cgcttgtcga ttatcctgct gtgggtgggc gtcatcgccc tgatcgccct
     1381 gctgggctgg ggactgctca acagcaccgc cacgcggccg gaagtgggcc agcaagcgcc
     1441 cgacttcgac gtggagttct tcgacggcta tgactgggac ggccggacga cagccaacct
     1501 ggccgacatg cgcggtcagg tcgtcgtgct caacttctgg gcctcgtggt gcgtcgaatg
     1561 tcgcgacgag acgccgatgc tggaagccgc ctggcagcgc tacgccggcc agggcgtcgt
     1621 cgttctgggc gtggcctatg ccgacgtgga accgaactcg ctggcctaca tgaaggagtt
     1681 cggcgtcagt ttcccccacg cgcccgatct gggcacggac atctcgcaga cctacgagat
     1741 caccggcgtg ccggagacat tcatcatcga caaggacggc atcatccgcc acgtgcagat
     1801 cggcgcagtg aacacggcca tgctcgacca gatcatcccg caattgctgg cccaatagcc
     1861 gccggaggaa gcatcgctgt gatcgctgga atcaccctcg gttcactttt gttgggcgtg
     1921 gccctggtcg tcgtggtcgt gctctacctg gcccggccgt tcgtgaccgc cgaagacgaa
     1981 gacgtccgcc tggcgcgcga agaggcggac agcctggcgc tgcgccgcga cgcgctgctg
     2041 cggcaggtgc gcgaactgga cgacgatatg gaggcggcca aggtcgcccc tgagctttat
     2101 cagcgcgtcc ggccacaact ggtcaagcag gcggccatca ttatgcagca actggacgcc
     2161 cgccaggcgg acgcccttac gcccgatctg gacgcgcaga tcgaggccgc cgtcaggcgg
     2221 ctgcgcacgc cggacgagat cgacgccgac atcgaggccg ccgtccgggc ggcgcgcggc
     2281 cggccggcgg
//
LOCUS       DAOSMQ010000505         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_47254_length_2281_cov_6.351303, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000505 DAOSMQ010000000
VERSION     DAOSMQ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47254_length_2281_cov_6.351303"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PK829_13425"
     CDS             <1..269
                     /locus_tag="PK829_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOU42261.1"
                     /translation="ERAQHESLQQLAAAEGRSMSEVIREAVAHYLVERDDETRKRQTL
                     TTIAALAQIREAIRLESGAFPEEFILNDRAEREEELWQRMGLNE"
     gene            266..712
                     /locus_tag="PK829_13430"
     CDS             266..712
                     /locus_tag="PK829_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOU42262.1"
                     /translation="MTIVIDANLVVALALPLPYADLATAAISHWIAQGEQLIAPMLME
                     YELVTAIRRGVASGRLREQRALPILYELRESGVQSIAPTAELHEQALYWAGRVSQHKA
                     YDAQYLALAAREDAHFWTADHRLAAAAQNAGLEWVHWIGDWNDATA"
     gene            709..1500
                     /locus_tag="PK829_13435"
     CDS             709..1500
                     /locus_tag="PK829_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HOU42263.1"
                     /translation="MSDILVESWLDLHDKLFQPAWQPTINRFRAQLAFRGLSDAHYDL
                     STSLMRLGQGYDRLEKHLLRNFRKYAHRDAVPDDSNWNWLTLAQHHGLPTRLMDWTFS
                     PYVAMHFATANLDAFTCDGVIWAVDYVKVHAALPERLQNELKQEGADLFTVEMLDRLA
                     PGIDGLAQPQDEPCLLFLEPPSIDDRVVNQYALLSVMSSATMRVDGWLAARPEFYARY
                     IIPAALKWEIRDKLDQANVTERVLFPGLDGLSIWLRRHYTPRAHG"
     gene            1535..>2281
                     /locus_tag="PK829_13440"
     CDS             1535..>2281
                     /locus_tag="PK829_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU42264.1"
                     /translation="MIPYDDFGGRGPVLHFAHPNAYTPGSFRQFLVPLTAHYHVVAAH
                     HRPLWPLSSDGDAAGPPEAAIDFTWDAIAGDLLRFLDQQGLAQVIGVGHSLGAVATMI
                     AARRQPERFRALVLIEPVFLPPHVLAAVRAHPQMAAERPFVLAALRRRDRWPSRADAF
                     ARFRDKAVFAGWSDEALWDYVNDGLGDDPATGEVVLRYPREWEAGFYARPPANVWDEL
                     PGLRRPTLAVRGTQSDTLYPEAWALWQNLQP"
BASE COUNT          382 a          801 c          719 g          379 t
ORIGIN      
        1 tagagcgcgc gcagcacgag agcttgcagc agttggccgc ggccgagggg cgcagtatgt
       61 ccgaagtaat ccgtgaggct gtggcccatt atctggttga gcgtgacgat gaaacgcgca
      121 agcggcaaac actgacgaca atcgccgcac tcgcccaaat ccgtgaagct attcgtctgg
      181 aatcaggcgc gttcccggaa gagttcatcc tcaacgaccg tgctgagcga gaagaggaac
      241 tctggcaaag gatggggctg aacgaatgac aatcgtgatc gacgccaatc tggtcgttgc
      301 cctggcgctg ccgctgccct atgcagacct ggcgacagcg gcgatcagtc attggatcgc
      361 gcagggtgaa caactcatcg cgccaatgct catggagtat gagttggtaa cggccattcg
      421 tcgtggcgtg gccagcggtc gattgcggga gcagagggcg ttgccgatac tctatgagct
      481 gcgcgaatct ggcgtccaga gcattgcccc aaccgcagag ttgcacgagc aggcgctcta
      541 ttgggccggg cgcgtcagcc agcacaaagc ctacgacgcc cagtatctcg ccctcgccgc
      601 gcgcgaagac gcgcatttct ggaccgccga ccaccgcctc gccgccgcgg cccaaaacgc
      661 cgggctggaa tgggttcact ggatcggcga ctggaacgac gcgacagcat gagcgacatt
      721 ctcgtcgaga gttggctcga cctgcacgac aaactgttcc agcccgcctg gcagcccact
      781 atcaaccgct tccgggcgca actggccttt cgcggcctgt ccgacgcgca ctacgacctg
      841 tccacctcgc tcatgcgcct aggccagggc tacgaccggc tagaaaagca cctgctgcgc
      901 aacttccgca agtatgccca ccgcgacgcc gtgcccgacg actccaactg gaactggctg
      961 acgttggccc agcaccacgg cctgcccacg cggctgatgg attggacgtt ctcgccctac
     1021 gtggccatgc acttcgccac ggccaacctg gatgccttca cctgtgacgg cgtcatctgg
     1081 gcggtggact atgttaaagt ccatgccgcg ctgccggagc gcttgcagaa cgaactgaag
     1141 caggagggcg ccgacctgtt cactgtggag atgctcgacc gccttgcgcc gggcatcgat
     1201 ggtctggccc agccccagga cgagccgtgc ctgctctttt tggagccgcc gtcgatcgat
     1261 gaccgcgtgg tgaatcagta cgcgctgctg tcggtcatgt ccagcgctac catgcgcgtg
     1321 gacgggtggc tggcagcgcg gccggagttc tacgcccgct acatcatccc cgcggcgctc
     1381 aagtgggaga ttcgcgacaa gctcgaccag gccaacgtca ctgagcgcgt tctcttcccc
     1441 ggcctcgatg gcctgagtat ctggctgcgg cggcactaca cgccccgcgc ccacggctga
     1501 gcgccggcta acagatcgat acgtatagag agccatgatc ccttacgacg acttcggcgg
     1561 ccgcggcccc gtgctgcact tcgcccaccc caacgcctac acgcccggct ctttccgcca
     1621 attcctggtc ccgctgaccg cgcattacca cgtcgtggcc gcccaccacc ggccgctctg
     1681 gcccctgtcg tccgacggcg acgccgccgg cccgccggag gccgccatcg acttcacctg
     1741 ggacgccatc gccggcgacc tgctgcgctt cctcgaccag caggggctgg cgcaggtcat
     1801 cggcgtcggc cactcgctgg gcgcggtggc gacgatgatc gccgcccgcc ggcagccgga
     1861 gcgcttccgg gcgctggtcc tcatcgagcc ggtctttttg ccgccgcatg tcctggccgc
     1921 cgtccgggca cacccgcaga tggccgccga gcggccattt gtcctggccg cgctgcgccg
     1981 ccgcgaccgc tggcccagcc gcgccgacgc ctttgcccgc ttccgcgaca aggccgtctt
     2041 cgccggctgg tcagatgaag ccctgtggga ctacgtcaac gatggcctgg gcgacgaccc
     2101 ggccacgggc gaagtcgtcc tgcgctaccc acgcgagtgg gaggcgggct tctacgcccg
     2161 gccgccggct aacgtctggg acgagctgcc cgggctgcgc cggccgacgc tggccgtgcg
     2221 cggcacgcag tcggacacgc tctacccgga ggcgtgggcg ctgtggcaga acctccagcc
     2281 c
//
LOCUS       DAOSMQ010000506         2273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_47482_length_2273_cov_4.027953, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000506 DAOSMQ010000000
VERSION     DAOSMQ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47482_length_2273_cov_4.027953"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PK829_13445"
     CDS             complement(<1..1006)
                     /locus_tag="PK829_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42265.1"
                     /translation="MTASLRHLAYEARPLAARAGLAVVAVLAALLIARLPLPLAAGLV
                     LGAAAVVLTLIQPLVGLGLALLLGPWGALQGVVLGNSLLDVGQLALLLTAAAWLGSCL
                     ARRRLVVPFTPLNLPWLLFVGVAAVSLLDAPSVALGLIELVKWLQMAFVVWLVVDLAA
                     PPRRPAVANILLALLLLAGLSQALIGIWQFGLRGDGPEHFIVLGHFYRAYGTFEQPNP
                     FGGYMNLTALPAIGLAMGLLISFRRPDSPLRAWSGRLWLAFVGLCAVATTLALVFSWS
                     RGAWLGFLAGAGVLALFSTRRPLAGLALAALAAVVVGGGLLLGVTTGFGPALSVADRL
                     G"
     gene            complement(1003..1947)
                     /locus_tag="PK829_13450"
     CDS             complement(1003..1947)
                     /locus_tag="PK829_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU42266.1"
                     /translation="MRVLITGAAGFLGSHLCDRFIAEGHTVVGMDNLITGNMDNIAHL
                     FGHERFGFIKHNVSNYIYVDGPLDAVLHFASPASPNDYLEHPIPTLKVGSLGTHNTLG
                     LAKHKGARYLLASTSEVYGDPQVNPQPESYWGNVNPIGPRGVYDEAKRFAEAMTMAYH
                     RYHGLETRIVRIFNTYGPRMRLNDGRVVPNFIYQALTNQPLTVYGRGVQTRAFQYYSD
                     LIEGIYRLLHSDESQPVNIGNPHTEITVQDLAERIVRLSGSQSEIICVEPTDDRFVDD
                     PQVRRPDITRARTLLGWEPRVPVDEGLAETIAYFRQRI"
BASE COUNT          467 a          763 c          743 g          300 t
ORIGIN      
        1 cgcccagacg gtcggccacg gagagcgccg ggccaaaccc cgttgtcacc cccagcagca
       61 acccgccgcc gacaacgacc gcggccagcg ccgccagggc caacccggcc agcggccgcc
      121 gcgtgctgaa cagcgccagc acacccgcgc ccgccagaaa ccccagccac gccccacggc
      181 tccatgagaa gaccaacgcc aacgtcgtcg ccaccgcgca cagacccaca aaggccaacc
      241 acagccggcc tgaccaggcg cgcagcgggc tgtctggccg gcgaaagctg atcagcagcc
      301 ccatcgctag gccaatggct ggcagcgccg tcagattcat gtagccgccg aaggggttgg
      361 gctgctcaaa cgtcccatag gcgcgataga agtggccgag gacgataaag tgctccggcc
      421 catcgccacg cagaccaaac tgccagatgc cgatgagcgc ctgcgatagg ccggccaaca
      481 gcaagagcgc caggagaata ttggccacgg ccgggcggcg cggcggggcg gccagatcga
      541 ccaccagcca gacgacaaaa gccatttgca gccacttgac taactcgatc aggcccaggg
      601 caaccgacgg cgcgtccagc agcgacaccg ccgccacgcc cacgaagagc agccagggca
      661 agttcagcgg cgtgaagggc acgacaaggc ggcggcgcgc cagacaggaa cccagccatg
      721 ccgccgcggt caacaagaga gccagctggc caacatccag caggctgttg cccaggacga
      781 cgccctgcag cgctccccac gggccaagca gtagcgccag gcccagcccc accagcggct
      841 ggatgagcgt caggacgacg gcggccgcgc ccagcaccag cccggccgcc agcggcagcg
      901 gcagccgggc aatgagcagc gcggccagaa cggccacgac cgccaggcca gcccgcgccg
      961 ccaacggccg ggcctcatac gccaagtgcc ggagagacgc ggtcaaatgc gttgccggaa
     1021 ataagcgatc gtctcggcca aaccctcgtc gaccggcacg cgcggctccc aaccgagcag
     1081 tgtgcgcgcg cgggtgatgt cgggccggcg cacctgggga tcgtctacaa aacggtcgtc
     1141 cgtcggctcc acacagatga tctccgattg gctgcccgac agccgcacga tgcgctcggc
     1201 caggtcttgg acggtgatct ccgtgtgcgg gttgccgatg ttgaccggct ggctctcgtc
     1261 ggagtgcagc aggcgataga taccctcgat caggtcagag tagtactgga acgcccgcgt
     1321 ctggacaccg cgaccataga ccgtcagcgg ttggttggtc agggcctggt agatgaagtt
     1381 gggcaccacg cggccgtcgt tgaggcgcat acgcgggccg taggtgttga agatgcgcac
     1441 aatgcgcgtc tccagcccat gatagcgatg gtaggccatg gtcatcgctt cggcgaagcg
     1501 cttggcctcg tcgtagacgc cgcgcgggcc gatggggttg acgttgcccc agtagctctc
     1561 cggctgcgga ttcacctgcg ggtcgccgta gacctcggag gtggaggcca acagatagcg
     1621 cgcacccttg tgcttggcca ggccaagcgt gttatgcgtg cccagggagc cgaccttcag
     1681 ggtggggatg gggtgctcca ggtagtcgtt ggggctggcc ggggaggcaa agtgcagcac
     1741 ggcgtcgagc gggccatcga cgtagatata gttactgacg ttgtgcttga tgaagccgaa
     1801 ccgctcgtgc ccaaataaat gagcgatatt gtccatgttg ccggtgatga ggttgtccat
     1861 gccaacgacg gtatgccctt cggcaatgaa ccggtcgcac agatgcgacc ccagaaagcc
     1921 ggccgcgccg gtgatgagta cacgcatgtc attctccagt aaacaggtaa cgggccacga
     1981 ggcgctaggg atacagtcgc catgcggcga tcacgtcgat ctcaaagggg gattttaccc
     2041 gcggtcagtg cggccggcga atcccggcgg ggcctttctt cgtattcgcc cctatggtcg
     2101 tctataattg tgtcccatga cagaagcaac caacacggct gccaacgccg acatccacga
     2161 gcggcgcaag gccgacacgg ccggccgcga acccggcagc gacatccacg agcggcgtaa
     2221 ggccgacacg gccggccgcg aacccggcag cgacatccac gagcggcgta agg
//
LOCUS       DAOSMQ010000507         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_47517_length_2272_cov_4.566080, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000507 DAOSMQ010000000
VERSION     DAOSMQ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47517_length_2272_cov_4.566080"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PK829_13455"
     CDS             <1..679
                     /locus_tag="PK829_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012264923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOU42267.1"
                     /translation="EGVAGGLDEYAYRTEKISRWVCRAVAEFAFQHAQQNEAKVFGGP
                     KYTVSPVYEGMFKEEMDAAATRHPDVRYEPQLIDATLALLIATSGEALVIPTLNRDGD
                     LLSDLVLQMFGSIAGSESMVISLNPEGVIDCVMAEAPHGTAPSLEGKNVANPMAMLLA
                     AAGLLGYMKEKELKAASRAIYEATLEAVYDGVRTADLHGTATTDQFTNEVIRRVRAKL
                     EVWSSLS"
     gene            complement(676..1896)
                     /locus_tag="PK829_13460"
     CDS             complement(676..1896)
                     /locus_tag="PK829_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU42268.1"
                     /translation="MNYGFESLPENDTLTKSLFHHTLMAFYAPARWPLALVVLALAAL
                     AAFVGAVWWWLSGETSPAVVAALLIALAMAVDVAILVAQRRARIAFGLWPGQIAALAL
                     PRTALALAVTPLGLWLGWPAAVALLAGIQLAGVVVLARATLVEPHRLTLSRLDVPTDR
                     LPAGTPPVRILHISDIHLERPGVREEQLLALAKETAPDLILITGDYVNLSYNLDGETH
                     AAVRCLLGQLSARYGVFAVLGTPAVDLEVAVPPLFDGLPVRLLRDEAVAVPVAGGTVT
                     LLGLDCHHDIARDAATLERVLASAPDNGPRILLYHSPDLMPDAVRRGIDLHLCGHTHG
                     GQVRLPLIGPLLTASSLGRRYVMGHYREGRTHLYVSRGIGFEGLSAPRVRLLCPPEVA
                     LLTLGSAGALAAAP"
     gene            2023..>2272
                     /locus_tag="PK829_13465"
     CDS             2023..>2272
                     /locus_tag="PK829_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU42269.1"
                     /translation="MDPKFPPRVPIAYLPTPIERLERLSRHLGGPELWIKRDDQTGLA
                     TGGNKTRKLEFLVADALAQGSDHLVTTGAPQSNHCRQTA"
BASE COUNT          420 a          749 c          792 g          311 t
ORIGIN      
        1 cgagggtgtg gccggcggcc tggacgagta tgcctatcgc accgagaaga tctcccgctg
       61 ggtctgccgg gctgtggccg agtttgcctt ccagcacgct cagcagaacg aggccaaggt
      121 cttcggcggg ccgaagtaca ctgtcagtcc ggtctacgag ggcatgttca aggaggagat
      181 ggacgccgcc gcgacacgcc accccgacgt gcgctacgag ccgcaactca tcgacgccac
      241 cctggccctg ctcatcgcca ccagcggcga ggcgctggtc atcccgacgc tcaaccgcga
      301 tggcgacctg ctctctgacc tggtgttgca gatgttcggc tccattgccg ggtcggagtc
      361 gatggtcatt tcgctcaacc cggagggcgt cattgactgc gtgatggccg aagcgcccca
      421 cggcaccgcg ccgagtctgg agggcaaaaa cgtggccaac cccatggcca tgctcctggc
      481 cgcggccgga ctgctgggct acatgaagga gaaggaactg aaggccgcca gccgggccat
      541 ctatgaggct acgctggagg cggtctacga cggtgtgcgc acggccgacc tgcacggcac
      601 ggccacgacc gaccagttca ccaatgaggt catccgccgc gtgcgggcca aactggaagt
      661 gtggtcgtcg ctcagctagg gcgcggcggc aagagcgcca gcagagccta gtgtgagcag
      721 ggccacctcc ggtggacaga gcagacgcac gcgcggcgcg ctcagcccct caaagccgat
      781 gccgcggctg acatagagat gcgttcgccc ctcgcggtag tggcccatga cgtagcgacg
      841 gccgagcgac gaggccgtca gcagggggcc gatgagcggc aggcgcacct gcccgccgtg
      901 ggtgtggccg cagaggtgca gatcgatgcc gcgccggacc gcgtcgggca tcaggtcggg
      961 cgagtggtag aggagaatgc gcggcccatt gtccggcgcg ctggccagca cgcgctccag
     1021 cgtcgccgcg tcgcgggcga tgtcgtggtg gcagtcaagg ccgagcaggg tgacggtccc
     1081 gccggccacg ggcacggcca cggcttcgtc acgcaacagg cgcaccggca ggccgtcgaa
     1141 gagcggcggg acggccactt cgagatcgac cgcgggcgtg cccaggacgg caaacacgcc
     1201 atagcgggcc gaaagctgac ccagcaggca gcgcacggcg gcgtgggtct ccccatcaag
     1261 gttataagac agattgacat aatcgccagt gatgagaata aggtcgggcg cagtctcttt
     1321 agccagcgcc agtagttgct cctcgcgcac gcccggacgt tccaggtgga tgtcgctgat
     1381 gtggaggatg cgcacgggcg gtgtgccggc gggcaggcgg tcggtgggca cgtcgaggcg
     1441 gcttagtgtc aggcgatgcg gctcgacgag ggtggcgcgg gcgagcacca cgacgccggc
     1501 aagctgaatg cccgccagca gcgccacggc ggcgggccag cccagccaca ggcccagcgg
     1561 cgtcacggcc agggccagcg ccgtccgtgg cagcgccagc gcggcgatct gccccggcca
     1621 cagaccgaag gcgatgcggg cgcggcgctg ggcgacgagg atggccacat cgacggccat
     1681 cgccagagct atgagcaggg ccgcgacgac agccggactt gtctcgccgg atagccacca
     1741 ccacactgct cccacaaacg ccgccagcgc cgccagcgcc agcacgacca gtgccaacgg
     1801 ccaacgcgcc ggcgcgtaga aggccatcag ggtatggtga aagaggcttt tggtcagggt
     1861 gtcgttctcg ggcaggctct caaaaccgta gttcattgac ccacattgta gcagaacgca
     1921 tcggcgggtg tggtatgcgc ccaatcgaca cggccagcaa atcggctaca ataccctctg
     1981 agccgccaag tgagattgcg ccgtccgttt gtgaagacgc ctatggaccc gaaatttcct
     2041 ccacgagtgc ccattgccta cctgcccacg cccatcgagc ggttggagcg cctcagccgt
     2101 catctgggcg ggccggagct gtggatcaaa cgcgatgacc agacgggcct ggccacgggc
     2161 ggcaacaaga cgcgcaagct ggagttcctc gtcgccgacg cgctggccca gggcagtgac
     2221 cacctggtga ccaccggcgc gccccagagc aaccactgcc ggcagacggc cg
//
LOCUS       DAOSMQ010000508         2268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_47650_length_2268_cov_3.116132, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000508 DAOSMQ010000000
VERSION     DAOSMQ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47650_length_2268_cov_3.116132"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..884)
                     /locus_tag="PK829_13470"
     CDS             complement(261..884)
                     /locus_tag="PK829_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOU42270.1"
                     /translation="MKPVLVTYATMAGSTADVAETIAAELRAAGLPVEVAPMAAVGAL
                     DGYSAVVAGGPMIMGWHRQAAGFLRQHRRDWARLPLAVFVMALSLTATAERTVAGVPV
                     FVDEQLPRPATTGGLRWRQRYAQLGRYVRPIVAAAGPAKPRSVGVFGGRMEYGRLKPW
                     AVLFVMGVIQAPAGDRRNWEAIRAWAAGLPAALGLAVEPALREMAVA"
     gene            complement(909..1436)
                     /locus_tag="PK829_13475"
     CDS             complement(909..1436)
                     /locus_tag="PK829_13475"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOU42271.1"
                     /translation="MSKIDPLIRDFLAQKKIAVVGVSDKRETGCNAAYQRFVAAGYTV
                     FPVNPRLSTYQGATCYPDLRAIPEKPDAVFILANPRVTDQIVAQCVELGVGHVWMHCM
                     MGVKPGAAANMTSVSADAVRVCRQNGIAVIPGTCPNQYLKPDFGHRLMRALAGVLGNL
                     RVDDSAPESAPRVAH"
     gene            complement(1652..>2268)
                     /locus_tag="PK829_13480"
     CDS             complement(1652..>2268)
                     /locus_tag="PK829_13480"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOU42272.1"
                     /translation="GNRTPNLSPTFIADLAAKLGLTFNPLQRETGEDEFGPEDVFHYI
                     YALLHSPTYRERYAEFLKIDFPRVPLTGDAALFRALAGLGRELTALHLLEAPQVNQFI
                     TRYPVPGDNRVAAGYPQFTHAAGEAAGRVHLNPTQYFDGVPPEVWAFHVGGYQVLDKW
                     LKDRRGRQLSYDDLTHYQRVVVALQRTLALMTAIDAAIPAWPLA"
BASE COUNT          356 a          734 c          858 g          320 t
ORIGIN      
        1 cctggctgaa ccgatggccg gcggcaacca gcgccggcac ggtcagatcg gcggcgcgcc
       61 ccagatagac cttgcgcagc ttgtcgcggt ggcggcgata gccgtaccag taatagtggc
      121 cccggcggct ctcgcggcga acgggtgtcg tcatgtggtc attctccgcg gggagtgtag
      181 ccgaaaacac gcctacctga caggtgagcg accgcggccg cgcacctgtc aggtgtttaa
      241 ccatgcggat gggcggtggg ctaggcgacg gccatttccc gtagcgccgg ctccaccgcc
      301 agtcccagcg ccgccggcaa cccggccgcc caggcgcgga tcgcctccca gttgcgccga
      361 tcgcccgccg gagcctggat gacgcccatg acgaacagca ccgcccaggg cttcagccgg
      421 ccatactcca tccggccgcc gaagacgccg acgctgcgcg gcttcgccgg cccggcggcg
      481 gcgacgatgg gccggacgta gcgccccagc tgggcatagc gctggcgcca gcgcagcccg
      541 ccggtagtcg ccggccgggg cagttgttca tcgacgaaga cgggcacgcc ggcgaccgtc
      601 cgctcggccg tggccgtcag gctgagggcc atgacgaaca cggccagcgg cagccgcgcc
      661 cagtcgcgcc ggtgttgccg cagaaagccg gccgcctggc ggtgccagcc catgatcatc
      721 ggcccgccgg cgacgacggc gctatagcca tccagcgcgc ccacggccgc catcggcgcg
      781 acctcgaccg gcagcccggc ggcgcgcagc tccgcggcga tcgtctcggc cacatcggcc
      841 gtcgagccgg ccatggtggc ataggtgacg agaacaggct tcatggccaa ccaacctccc
      901 tcgccgcgct agtgggccac gcgcggggcc gattcggggg cgctgtcgtc cacgcgcagg
      961 ttgcccagga cgccggccag cgcccgcatc aggcggtggc cgaagtcggg cttcaggtac
     1021 tgattggggc aggtgccggg gatgacggcg atgccgttct ggcggcagac gcgcacggcg
     1081 tcggccgaga cgctggtcat gttggccgcc gcgccgggct tcacgcccat catgcagtgc
     1141 atccatacgt ggccgacgcc cagctcgaca cactgggcga cgatctggtc ggtcacccgg
     1201 gggttggcca ggatgaagac ggcatccggc ttctccggaa tggcccgcag gtcggggtag
     1261 caggtcgcgc cctggtaagt agacaggcgc ggattgacgg ggaagaccgt gtagccggcg
     1321 gcgacgaagc gctgataggc ggcattgcag cccgtctcgc gcttgtcgga cacgccgacg
     1381 acggcgatct tcttctgggc gaggaaatcg cgaatgaggg gatcgatctt ggacatggtg
     1441 cagtttcctc cgcgccgcgg gccgcgaggg cggctggcgg gcgtaaccag gccacaaaga
     1501 acagcggcgg ggcgtggccg ggcgacgacg gcaacccgcc taattaacta ctatagatca
     1561 aagcgggcgc gggcaatagt aacgaaggtc atgacctggg catgacaggc ccacgcgccg
     1621 gggcggcttg gccggctggg cggcggcggc gtcaggccag gggccaggct ggaatggcgg
     1681 cgtcgatggc ggtcatgagc gccagcgtgc gttgcagggc gacgacgacg cgctggtaat
     1741 gggtcaggtc gtcataggac aactgccggc cgcggcggtc cttcagccac ttgtcgagca
     1801 cctgatagcc gccgacgtgg aacgcccaca cctccggcgg cacgccgtcg aagtactgcg
     1861 tcgggttgag atggacccgg ccggccgctt cgccggcggc gtgggtgaat tgggggtagc
     1921 ctgcggccac gcggttgtcg ccgggcacgg ggtagcgggt gatgaactgg ttgacctgcg
     1981 gcgcttcgag caggtgcagg gccgtcagtt cgcgccccag cccggccagc gcccggaaga
     2041 gggccgcgtc gccggtcagg ggcacgcggg ggaagtcgat cttcaggaac tcggcatagc
     2101 gctcgcggta ggtggggctg tggaggaggg cgtagatgta gtggaaaacg tcttcggggc
     2161 cgaactcgtc ttcgcccgtc tcgcgctgga gggggttgaa ggtcaggccg agtttggccg
     2221 ccagatcggc gatgaaggtg ggggagaggt tgggggtgcg gttgccgg
//
LOCUS       DAOSMQ010000509         2266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_47705_length_2266_cov_3.217549, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000509 DAOSMQ010000000
VERSION     DAOSMQ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47705_length_2266_cov_3.217549"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PK829_13485"
     CDS             complement(<1..895)
                     /locus_tag="PK829_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42273.1"
                     /translation="MTQTAIDQLRALHDELGAHADLVEMLDARDRVYARYGRAFAPEN
                     LPELTAGDYLSFLRFENNGHWSSIHRYGSRTTSDMPALRRALGVLTDEQRPLAERYDE
                     ATGGAVKGIGRAIATPILHVVYPDRYGVWNSQSHAGLQAIGALPRFEPGWTDGQKYEA
                     VNETLLALAEALRIDLWTLDAVWEVLRRRRPLAPPFDSIFIDRAEAEWAFDLFAETAT
                     RLGGGPDDPRIAVTLPHDQTMMRLNFGPWMAIDVSRKRGKMDIVLPIAEAETLGLTEH
                     DEPFRNDPGMAVVFIPLESARD"
     gene            complement(992..2140)
                     /locus_tag="PK829_13490"
     CDS             complement(992..2140)
                     /locus_tag="PK829_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42274.1"
                     /translation="MPAVMSPVDADAIPLYTGPRAGDSAAHPFFTADAAARSLNRARL
                     VELPGVTGAAELLGRSASELLSAAAVTALVRAAADRLALDPDDPALLRAFDAAFDAPP
                     TRPAALAVAEAVGRRLGALLLMLWRGDAANRAARPEWDEAHWAFWRAVERVVVGGGLL
                     AGRLGEATVPAAGAFLAAAGCPIAVERSPYGNAIAIAGLARHAPAGAERMLLFDFGHT
                     AVKCGLATYRQGALAGLERRPSLPPPCEDGWPAHAGAAWARPRWERMAAAITAAWAAE
                     VAPGQGERVAVGLCLASHLHDGHPIGRDRGCYTALGELAPHLATFLRDDLRARLGLFR
                     ALAVLHDGLAAASTRAGEPRTVVLTLGTAIGAGYPLGEVGLRAMKLGL"
BASE COUNT          360 a          818 c          774 g          314 t
ORIGIN      
        1 tgtctcgcgc gctttccagc gggatgaaga ccacggccat gccgggatca ttcctgaacg
       61 gctcgtcgtg ttcagttaac cccagagtct cggcctctgc aatgggcaac acgatatcca
      121 ttttcccacg tttccgtgag acatcgatgg ccatccacgg gccgaagttg agccgcatca
      181 ttgtttgatc atggggcagc gtcacggcaa tgcgcgggtc atctggccca ccgcccagcc
      241 gcgtcgccgt ttcggcgaag agatcgaagg cccactccgc ctcggcgcga tcaataaaga
      301 tactgtcgaa gggcggcgcc aggggccgcc gccggcgcaa gacctcccag acggcgtcaa
      361 gcgtccacag atcgattcgc agcgcctccg ccagagccag cagcgtctcg ttgaccgcct
      421 catacttttg cccatctgtc cagcccggtt caaaccgtgg cagcgcgccg atggcctgta
      481 gcccggcgtg gctttggctg ttccagacgc cgtaacggtc ggggtagaca acgtggagga
      541 tgggggtggc gatggcccgg ccgatgcctt tgaccgcgcc gccggtcgcc tcgtcgtaac
      601 gctcggccaa cggccgctgc tcgtcggtca ggacgcccaa cgcccgccgc aaagccggca
      661 tatcgctggt tgtgcggctg ccgtagcggt gaatacttga ccagtggcca ttgttctcga
      721 agcgcaggaa ggacaggtag tcgccggccg tcaattcggg caggttttcg ggggcaaaag
      781 cccgcccgta gcgggcatag acccggtcgc gcgcgtccag catttccacc aggtcggcgt
      841 gcgcgcccaa ctcgtcgtga agggcgcgca gttggtcaat ggccgtttgt gtcatgtctg
      901 ctaacctctt ttgcccccat gagcaacaca tcgaaacagg gtgtccctct cgttgtggcc
      961 ccatacacgc ccaagtatag acacatagcc cttacaaacc gagtttcatc gcccgcaacc
     1021 ccacctcccc cagcggatac cccgccccaa tcgccgtccc cagtgtcagc acgacggtgc
     1081 gcggctcgcc ggcgcgggtg ctggccgcgg ccaggccgtc gtggaggacg gccagcgcgc
     1141 ggaagaggcc tagccgcgcc cgcaggtcat cgcgcaggaa cgtggccagg tgcggggcca
     1201 gttcgcccag cgccgtgtag cagccgcggt cgcggccgat ggggtggccg tcgtgcaggt
     1261 ggctggccag acacagtccc acggcgaccc gctcgccctg gcccggcgcg acctccgcgg
     1321 cccacgcggc ggtgatggcc gcggccatgc gctcccagcg cggccgcgcc cacgccgcgc
     1381 cggcgtgcgc gggccagccg tcctcacacg gcggcggcaa agatggccgc ctctccaggc
     1441 cggccaatgc gccctgacgg taggtggcca ggccgcactt gaccgccgtg tggccaaaat
     1501 cgaacaggag catgcgctcg gcgccagctg gcgcgtggcg ggccaggccg gcgatggcga
     1561 tggcattgcc gtagggcgag cgctcgacgg cgatggggca gccggccgca gccagaaacg
     1621 cccccgccgc gggcacggtc gcctcgccca gccgcccggc cagcagcccg ccgccgacaa
     1681 ccacccgctc gaccgcccgc cagaaagccc agtgggcttc gtcccactcc ggccgcgcgg
     1741 cgcggttggc cgcgtcgccg cgccagagca tcagcagcag cgcccccagc cgccggccaa
     1801 ccgcctcggc cacggccagc gccgccgggc gggtcggcgg cgcgtcgaag gcggcgtcga
     1861 aggcgcgcag cagcgccgga tcgtcggggt cgagagccag acggtcggcc gcggcgcgca
     1921 cgagggcagt caccgccgcg gccgagagca gttccgacgc cgagcggccg agcagttccg
     1981 cagcgccggt aacgcccggc aattcgacaa gccgcgcccg gttgagcgaa cgggcggcgg
     2041 cgtcggcagt gaagaagggg tgggcggcgc tgtccccggc gcgcgggccg gtgtagagcg
     2101 ggatggcgtc ggcgtccacg ggcgacatca cggctggcaa tcgacgtcct ggacggcgca
     2161 ggccacctcg acccagccgg ccagatagac gccgttggac tgtagctccc cctcgaccgt
     2221 caccggcagg cgcgcgccgc tggccggatc atacatgccg atccag
//
LOCUS       DAOSMQ010000510         2265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_47723_length_2265_cov_5.157919, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000510 DAOSMQ010000000
VERSION     DAOSMQ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47723_length_2265_cov_5.157919"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK829_13495"
     CDS             complement(<1..233)
                     /locus_tag="PK829_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOU42275.1"
                     /translation="MTDQVTLTIDGQEVTVPAGTTILNAAASVGITIPAVCYHPHLTA
                     NGLCRVCSVDAGRRLQAAACVTQVSPGMVVNTQT"
     gene            complement(379..1230)
                     /gene="fdhD"
                     /locus_tag="PK829_13500"
     CDS             complement(379..1230)
                     /gene="fdhD"
                     /locus_tag="PK829_13500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOU42276.1"
                     /translation="MDIHIHTVGGQGQVAPDSLQPGAGARPAHYLSLNGDEPQAIDGA
                     VVEEALVCITVNGEELATFMCTPRDLPEMALGFLYNEGLIAGRDDVRALTVSAHNTCV
                     DVWLRDANYRPPRRAIITAGCGGGLTFDDLSTAHAPLTADLRATPAQLAALMRRLHLG
                     AELYQLARGIHTAALADPCAPGHECFLLQMEDLGRHNCLDKLQGAALLAGIPTRNRIL
                     LSSGRISSEMINKARRLETPIVCSRTSPTSLSVALAEAWNLTLVAYLRQDRMRVYTHP
                     ERVISKQ"
     gene            complement(1232..>2265)
                     /locus_tag="PK829_13505"
     CDS             complement(1232..>2265)
                     /locus_tag="PK829_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU42277.1"
                     /translation="ADESEPGTFKDRALMEQDPFALVEALTLAAYTVGAENGWIFLRG
                     EYPRCYARLQNAIDKAREAGYLGRDILGRKGFNFDIQLRLGAGAYICGEETALFEAIE
                     GKRGFPRIKPPFPTTHGLFHQPTAINNVETLAAALSVINIGVEQWHKLGTPGSPGTKW
                     FCVSGRVLRPGVYEVPFGLTVRQLIQMAGGVIGNEIQAVLLGGAAGVFIGPRRLDTPL
                     TYEDAKAQGFPLGSGVIMVFDEAVDLREILYMLARFFAHESCGKCYPCQLGTQRQLEI
                     IERVLRYGGPLPDDRQMLQDIGLAMTQTSLCGLGQTAASAVLSALERWPELFAPTGRA
                     NGQTPTGLL"
BASE COUNT          369 a          712 c          783 g          401 t
ORIGIN      
        1 gtctgggtgt tgacgaccat gcccggcgac acctgggtca cgcaggcggc cgcttgcagc
       61 cgccgcccgg catcgaccga gcacacccgg cacaggccgt tggccgtcag gtggggatgg
      121 tagcagacgg cggggatggt gatgcccacc gaggcggcgg cgttgaggat ggttgtgccc
      181 gccggcacgg tcacttcctg cccgtcgatg gtgagggtta cttggtcggt catgggtcgg
      241 tgtccagtat tcagtgtcca gtatccagtg tccagtattc agtgtccagt atccagtgtc
      301 cagtattcag tgtccagtat tcagtgtcct atattcggtc ttctgcctga agactggata
      361 ccggatactg cttactgctt actgcttact gataactctc tccggatgcg tatagacacg
      421 catccgatcc tgtcgcaaat aggccaccag agtcaggttc cacgcctcgg ccagggccac
      481 ggacaggctg gtgggcgagg tgcgcgaaca gacgatgggc gtctccagac ggcgagcctt
      541 gttgatcatc tcgctgctga tgcgcccgct gctgagcaga atacggttgc gggtcgggat
      601 accggccagc agcgccgcgc cctggagctt gtcgaggcag ttgtggcggc cgaggtcttc
      661 catttgcagc aggaaacact cgtggcccgg cgcgcacggg tcggccaggg cggcggtgtg
      721 gatgccgcgg gccaactggt acagctccgc gccgaggtgc agccggcgca tgagcgcggc
      781 cagttgtgcc ggcgtggccc gcaggtcggc cgtcagcggc gcgtgggcgg tcgataggtc
      841 gtcgaacgtc agtccgccac cgcagccggc ggtgatgatg gcccggcgcg gcggccggta
      901 gttggcgtcg cgtagccaga catcgacgca agtgttgtgg gccgagacgg tcagggcgcg
      961 cacgtcgtcc cgcccggcga tgaggccctc gttgtagagg aagcccaggg ccatctctgg
     1021 caagtcgcgc ggggtacaca tgaaggtggc cagttcctcg ccgttgacgg tgatgcagac
     1081 cagcgcctcc tccaccaccg cgccgtcgat ggcttgcggt tcgtcgccgt tgaggctgag
     1141 atagtgcgcc gggcgcgctc ccgcgccggg ctgaaggctg tccggagcga cctggccctg
     1201 gccgccgacg gtgtggatat gaatatccat tctacaacag ccccgtgggc gtctgcccgt
     1261 tggcgcggcc ggtgggggca aacaactccg gccagcgctc cagcgcgctc agcacggccg
     1321 aggcggctgt ctggcccagg ccacacagcg aggtctgggt catggccagg ccgatgtcct
     1381 gaagcatttg cctgtcgtca ggcagcgggc cgccatagcg caggacgcgc tcgatgatct
     1441 ccagttgccg ttgcgtgccc aactggcagg ggtagcactt gccacaactc tcgtgggcga
     1501 agaagcgggc cagcatgtag aggatttcgc gcaagtctac agcctcgtcg aagaccatga
     1561 tgacgccaga acccagggga aagccctgcg ccttggcgtc ttcgtaggtt agcggcgtgt
     1621 ccagccggcg cgggccgatg aaaacgccgg ccgcgccgcc cagcagaacc gcctgaatct
     1681 cgttgccgat gaccccgcca gccatctgaa tgagttggcg gacggtcagg ccaaagggca
     1741 cctcgtagac gccggggcgc agaacgcgcc cgctgacgca aaaccacttt gtgcccggcg
     1801 agccgggcgt gcccagcttg tgccactgct caacaccgat gttgatgacc gacagcgccg
     1861 cggccagcgt ctcgacgttg ttaatggccg tcggttggtg gaacaggccg tgggtggtcg
     1921 gaaagggcgg cttgatgcgc ggaaagccac gtttgccctc gatggcctcg aagagcgctg
     1981 tctcctcgcc gcagatgtag gcccccgcgc ccaggcgcag ctggatgtcg aagttgaaac
     2041 ccttgcggcc caggatgtcg cggcccagat agccggcctc gcgggccttg tcgatggcgt
     2101 tctgcaagcg cgcgtagcag cgcgggtact cgccgcgcag gaaaatccag ccattctccg
     2161 cgccgaccgt gtaggccgcc agtgtcaggg cctcgaccag ggcaaagggg tcttgctcca
     2221 tcagcgcccg gtctttgaac gtgcccggct cgctctcgtc ggcgt
//
LOCUS       DAOSMQ010000511         2245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_48265_length_2245_cov_4.106849, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000511 DAOSMQ010000000
VERSION     DAOSMQ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48265_length_2245_cov_4.106849"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PK829_13510"
     CDS             <1..731
                     /locus_tag="PK829_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOU42278.1"
                     /translation="DFEVVSGTAGATTINSYAPAELRDGAALALDVAAAAIGVNEARL
                     SPFPYTELDIVTTPTAALGIEYPGLIVGTLRMYDIQATNQAGVGYDDILESTTAHEVM
                     HQWFYNLIGNDQLDEPWLDESMAGYQTYRYYIDRYGQSAGDGYFANFAGRWESVDRAE
                     IPIGLPVRAYEGPEYGAIVYGRGPFFLRALEEKMGRQTFDAFLRDYAATFRWGISTTA
                     DFRALAEAQCGCDLEPLFAAWVAE"
     gene            complement(1148..1585)
                     /gene="aroQ"
                     /locus_tag="PK829_13515"
     CDS             complement(1148..1585)
                     /gene="aroQ"
                     /locus_tag="PK829_13515"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019503130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOU42279.1"
                     /translation="MRILILHGPNLNLLGQREPEVYGRWTLAEINEMLVAEASKRGVE
                     LRVLQSNHEGTLIDAIHEARPWANGILINPGALTHYSYALRDAIAAAEIPTVEVHLSN
                     IHAREEFRHRSVIAPVCVGQISGFGRHSYRLGLAALLDSLLER"
     gene            complement(1858..>2245)
                     /locus_tag="PK829_13520"
     CDS             complement(1858..>2245)
                     /locus_tag="PK829_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOU42280.1"
                     /translation="YERGRRAVLNLGHTFGHAIEQVSGYAIRHGDAVAIGLAAAARLS
                     AALEECSPSLPHLVEMTLARLGLPTHFPPTLDPAALFAAMGTDKKKLAGRLRFVLLHD
                     IGDVFIRDDVPAAVVVDVLAGLGAGE"
BASE COUNT          415 a          739 c          699 g          392 t
ORIGIN      
        1 gcgactttga ggtggtcagc ggcacggccg gcgcgacgac gatcaacagc tacgccccgg
       61 ccgagctacg cgacggcgca gccctggccc tcgacgtggc cgcggcggcc atcggcgtca
      121 acgaggcccg cctgtcgccc ttcccctaca cggaactcga catcgtcacc acgcccacgg
      181 cggcgctggg catcgaatat cccggcctca tcgtcggcac gctgcgcatg tacgacatcc
      241 aggccaccaa ccaggccggc gtgggctacg acgacatcct cgaatcgacc acggcccacg
      301 aggtcatgca ccagtggttc tacaacctca tcggcaacga tcagctcgat gagccgtggc
      361 tggacgagtc gatggccggc taccagacct accgctacta catcgaccgc tacggccagt
      421 ccgcgggcga cggctacttc gccaacttcg ccggccgttg ggagagcgtt gaccgggccg
      481 agattcccat tggcctgcct gtacgcgcct acgaagggcc ggagtatggg gccatcgtct
      541 atggccgcgg cccctttttc ctgcgcgctc tggaggagaa gatggggcgg cagacattcg
      601 acgcctttct gcgcgattac gcggcaacgt tccgctgggg catcagcacc acggccgact
      661 ttcgcgccct ggccgaggcc cagtgcggtt gcgatttgga gccgcttttc gccgcctggg
      721 tggctgagta atatgtcggg gatcaattga ctggaaaatc gtaagtgaca ccgtaacaat
      781 tgtcgtgtat agtaccttca tatcctgtag gaatgacgcg gcgcaaacaa cgagctacga
      841 agccacatcg ccgccagcac cataataact tcacaccacc accgcactac tgcgtgacta
      901 tcagggccgt cacatcgcca caccatcgtc acacgcaccg caccaactct ggggggccac
      961 cggccttatg gcgtcatcgt cgaaccatca cagagcacca tcgcgccacc gtcctgacct
     1021 gatagtctcc gcgtcattca ccaccattgc caacatcgcc cacaggatcc acaaagacaa
     1081 agaccgagcc agccactcgg tcttttcttt ttggccgcag cgcgacggtt ggccgcggcg
     1141 cgacggctta acgttccagc aggctgtcca gcagcgccgc caggcccagc ctgtagctgt
     1201 gccggccgaa gccgctaatc tgccccacgc agacaggggc gatgactgag cggtggcgga
     1261 actcctcgcg ggcgtggatg ttggacagat gcacctcgac cgtagggatc tcggccgcgg
     1321 cgatagcatc acgcagggcg tagctataat gggtcagcgc gccggggttg atgaggatgc
     1381 cgttggccca cggccgcgcc tcatggatgg cgtcgatgag tgtgccctcg tgattcgatt
     1441 gcagcacgcg cagctccaca ccgcgcttgc tcgcctcggc aacgagcatt tcgttgatct
     1501 cggccagcgt ccagcggccg tagacctccg gttcgcgctg gcctagcagg ttcaggttgg
     1561 ggccgtgaag gattaggatg cgcatggggt aggggatagg ggatagggat tagggattag
     1621 ggattaggtt ccaggttcca ggttccaggt tccagggaag agcgttcgtc aacctggccc
     1681 ctatcattat ccgtcattgg gcgtcatctg tcatcagtgg ccgacaatta ggggtcaggt
     1741 tccagggaag agtgctcacg ccaaggacgc ccagggggca aaggcgcaaa gaagaagctt
     1801 tcttcctttg cgcctctgct tcctttgcgg cttggcgtga gcctcttccc ctggaaccta
     1861 ttccccagcc cccagccccg ccagcacgtc cacgacgacc gccgccggca cgtcgtcgcg
     1921 gatgaagacg tcgccgatgt cgtggagcag gacgaaacgt aggcggccgg ccagcttctt
     1981 cttgtccgtg cccatggcgg cgaagagggc cgccgggtcg agcgtcggcg ggaagtgggt
     2041 cggcagacct agccgggcca gggtcatctc gaccaggtgg ggcaacgacg gcgagcactc
     2101 ctctagcgcc gccgacagcc gcgccgccgc ggccaggccg atggccacgg cgtcgccgtg
     2161 gcggatggcg tagcccgata cctgctcgat ggcgtggcca aaggtatggc ccaggttcag
     2221 cactgcccgc cggccgcgct catac
//
LOCUS       DAOSMQ010000512         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_48499_length_2237_cov_4.412466, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000512 DAOSMQ010000000
VERSION     DAOSMQ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48499_length_2237_cov_4.412466"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK829_13525"
     CDS             <1..230
                     /locus_tag="PK829_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42281.1"
                     /translation="HGEREFGREGGHGPRHGRWHEHSQGERPPLSEAERHAARHVRHE
                     VRRIMRAAEQAGLSPERVARLVRRQTRHDFD"
     gene            347..823
                     /locus_tag="PK829_13530"
     CDS             347..823
                     /locus_tag="PK829_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42282.1"
                     /translation="MKITTPQGYIIELTTEEAETLFPELLRDVRRQLGGMGEGGRDQS
                     PQRLLRRVLRRHRIPPTQLALYRALYEAGDDGLDFNSLAATIGRSAEELSGVLGGLGR
                     RVNATSGIGALPEPPGVTLLFEQKESAPPGGGWGWAMRPELRAALEEMGLPWAGEE"
     gene            1103..1840
                     /locus_tag="PK829_13535"
     CDS             1103..1840
                     /locus_tag="PK829_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42283.1"
                     /translation="MNKRLMLIAILLLNGLAAATTVLARELSEPAHTMTMPATASADL
                     VRLVQPAEQALRLVGSPAFPTAAGWATVDAAGREIKVEVSGLAALAGQTIEVTLGGAR
                     AGSMTVGADGQGALRVEGDSAGAILAAANGTALELRAGAAVIASSQLQTDDNGDDNAN
                     DNGDDNANDNGDDNANDNGDDNANDNGDDDTNDNGDDNANDNGDDNANDNGDDNANDN
                     GDDHGGHDDHGGDDNSGAGHDDHSGHN"
     gene            1946..>2237
                     /locus_tag="PK829_13540"
     CDS             1946..>2237
                     /locus_tag="PK829_13540"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOU42284.1"
                     /translation="MDDQTLLRKARALDREALGLIYDQHYQSVYRYVSFRVSDTQTAE
                     DLAAEVFTRFLAALRDRGALPNTVVGWLFGTAQNVLREHYRKQKQMNWSELDD"
BASE COUNT          434 a          778 c          732 g          293 t
ORIGIN      
        1 gccacggcga gcgcgagttc ggccgcgaag gcggccacgg cccgcgccac ggccgctggc
       61 atgagcacag ccagggcgaa cggccgccgc tgagcgaggc cgagcgccac gccgcccgtc
      121 acgtccgcca cgaggtgcgc cgcatcatgc gtgccgccga acaggccggg ctgtcgcctg
      181 agcgcgttgc ccgccttgtc cgccgccaga cgcgacacga cttcgactag caattgacgt
      241 ggcgcgattc tccgaatcgc tcttcgtgcc gcgccagtcg ggcgcagaac gcgattctgg
      301 agaatcgcgc cacatgacgg tctattggtt ataacaggcg acaattatga agatcacgac
      361 cccccagggc tatatcatcg agctgacgac cgaagaggcc gagacactat tcccggagct
      421 gctgcgcgac gtgcggcgac agttgggcgg catgggcgag ggcggccgcg accaatcgcc
      481 gcaacggttg ctgcgccgcg tcctgcgccg ccatcgcatt ccccccaccc aactggccct
      541 ctaccgcgcg ctctacgagg ccggcgacga cgggctagac ttcaactcgc tggcagcgac
      601 gattggccgc tcggccgagg aactcagcgg cgtgctcggc gggctgggcc ggcgcgttaa
      661 cgccacctcc ggcatcggcg cgctgccaga gccgcccggc gtaaccctcc tcttcgagca
      721 gaaggagagc gctccgcccg gcggcggctg gggctgggcc atgcgccccg agttgcgcgc
      781 cgcgctggag gagatgggtc tgccctgggc gggcgaggag tagaccgcta tctcagggcc
      841 gggcgctttc ttgctcggac acgccgccca gaaggcgcgc cgcgttccgg caagtacgcc
      901 gcgccgcgcc ggccagcctc agtgccgggc actcaataaa gtgcccggca cttttttgtg
      961 cgcctgaagc taggcccatt cgctctctga agcagccaat ttgcaaagcg cgatgcccgc
     1021 cccgttactt tcccgccgcg gccgacactt tctacacaga cgaccgcgcg gcgcgaaaac
     1081 aacgtaggag aacgggaacg acatgaacaa gagactgatg ctgatcgcga ttttgttgct
     1141 gaatgggctg gccgcggcca cgacggtgct ggcccgcgaa ctgagcgagc cggcccacac
     1201 catgaccatg cctgccacag cgagcgccga tctggttcgc ctggtgcaac cggccgagca
     1261 ggcgctgcgc ctcgtcggca gcccagcctt tccgacggcg gccgggtggg ccacagtgga
     1321 cgccgccgga cgcgagatca aggtcgaagt gagcggcctg gccgcgctgg ccgggcagac
     1381 gatcgaggtc accctcggcg gggcgcgcgc cggcagcatg accgttggcg cggacggaca
     1441 gggcgcgttg cgcgtcgagg gcgacagcgc cggggccatt ctggccgcgg ccaacgggac
     1501 ggcgctggag ttgcgcgccg gcgcagccgt cattgccagc agccagttgc agaccgacga
     1561 caacggcgac gacaacgcca acgacaacgg cgatgacaac gccaacgaca acggcgacga
     1621 caacgccaat gacaatggcg atgacaacgc caatgacaac ggtgacgacg ataccaatga
     1681 caacggcgac gacaatgcca acgacaatgg cgatgacaac gccaacgaca acggcgatga
     1741 caacgccaac gacaacggcg acgaccacgg cggccacgac gaccacggcg gcgacgacaa
     1801 ttccggcgcc ggccacgacg accacagcgg ccataactag gccctggaca accaccccgc
     1861 gccagagagc cggccgcccg cgcaatcagg cggccggctc tccaccctga ccgcgcggct
     1921 gggaacgtgt taatatcgcc ctgctatgga cgaccagaca ttgctacgga aggcccgcgc
     1981 cctcgacagg gaagcgctgg gcctcatcta cgaccagcat taccaatcgg tctaccgcta
     2041 tgtatccttc cgggtcagcg atacgcaaac ggccgaagac ctggccgccg aggtcttcac
     2101 ccgcttcctg gccgccctgc gcgaccgggg cgcgctgccc aacacagtcg ttggctggct
     2161 gttcggcacg gcgcagaatg tcctgagaga gcattaccgc aagcaaaagc agatgaactg
     2221 gagtgagttg gacgacg
//
LOCUS       DAOSMQ010000513         2235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_48557_length_2235_cov_4.073394, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000513 DAOSMQ010000000
VERSION     DAOSMQ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48557_length_2235_cov_4.073394"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PK829_13545"
     CDS             complement(<1..890)
                     /locus_tag="PK829_13545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HOU42285.1"
                     /translation="MLLTHTDHGLYCAAGDFFIDPWQPVARAVITHAHGDHARAGSAA
                     YLCATPGEGILRLRLGPEARIETAAYGQTLRLNGVAVSFHPAGHILGSAQVRVAHGGE
                     VWVISGDYKTEPDPTCAPFETVAADTFITEATFGLPIYRWQPSARVFAEVNAWWRANQ
                     AAGRTSVLFAYALGKAQRVLAGVDAAIGPIFVHGAVHRLNAAYCAAGVPLPAWRYVGD
                     APADQDWSRALVIAPTSAQGTPWLRRFGDVSAAFASGWMRVRGQRRRRSVDRGFVLSD
                     HADWPGLLAAITATGAARVLV"
     gene            912..1880
                     /locus_tag="PK829_13550"
     CDS             912..1880
                     /locus_tag="PK829_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU42286.1"
                     /translation="MMLFDLPLEELQTYRPPRPEPADFDAFWAAALAAARATPAAATF
                     TPIDAGLRLIDVDDVSFRGYGGQPIRGWLLRPRGVDGPRPCVVEYIGYGGGRGHPHDW
                     LLFAAAGYAHFVMDTRGQGSVWRRGDTADPEGDGGNPQHPGFMTRGVLSPSTYYYGRL
                     YIDAVQAIAAAREHPAVDAGRVAVCGGSQGGGVALAAAGLVGDLAAALIDVPFLCHFR
                     RATTITDADPYGEIVRFCKIHRDRVATVFATLDYFDGINFAARGRAPALFSVGLMDDV
                     CPPSTVFAAYNHYAGPKEMRVWPFNRHEGGEGYQTAERLRFLRELW"
     gene            1938..2084
                     /locus_tag="PK829_13555"
     CDS             1938..2084
                     /locus_tag="PK829_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42287.1"
                     /translation="MDIVPIAGGRVTIDGQSFEVSGPQVSSAAALLARLSAAGIDPAT
                     LAAR"
BASE COUNT          326 a          741 c          835 g          333 t
ORIGIN      
        1 acgaggacgc gggccgcgcc cgtggccgtg atggctgcca gcaggccggg ccagtcggcg
       61 tggtcggaca ggacaaagcc gcggtcgacc gagcggcggc ggcgctggcc acggacgcgc
      121 atccagccgg aggcgaaggc ggcggaaaca tcgccgaagc ggcgcagcca cggcgtgccc
      181 tgggccgaag tcggggcgat gaccagcgct cgcgaccagt cctgatcggc cggagcgtcg
      241 ccaacatagc gccaggccgg caggggcacg cccgcggcgc aataggcggc gttgaggcgg
      301 tggacagccc cgtggacgaa gatcggcccg atggccgcat cgacgccggc caggacacgc
      361 tgggccttgc ccagagcgta ggcaaacagc acgctcgtgc gcccggcggc ctggttggcc
      421 cgccaccagg cgttgacctc ggcgaagacg cgggcggaag gctgccagcg gtagatgggc
      481 aggccgaagg tggcttcggt gatgaaggta tcggcggcga cggtctcgaa cggcgcgcag
      541 gtggggtcgg gttcggtctt gtagtcgccg ctgatcaccc agacctcgcc gccatgagcg
      601 acgcgcacct gggccgagcc gaggatgtgg ccggcgggat ggaaggagac ggccacgccg
      661 ttgaggcgca gcgtctggcc ataggcggcc gtctcgatac gcgcctccgg ccccaggcgc
      721 aggcgcagga tgccctcgcc gggcgtggcg cagaggtagg cggccgagcc ggcacgggcg
      781 tggtcgccgt gggcatgggt gatgacggcg cgggcgaccg gctgccacgg gtcgatgaag
      841 aagtcgcccg cggcgcagta aaggccatga tcggtatggg tcaggagcat cgctcaggat
      901 tataattgac gatcatgctt tttgatctac ctctagaaga actccaaact tatcggccgc
      961 cgcgtccaga gccggccgac ttcgacgcct tttgggcggc ggcgctggcg gcggcgcggg
     1021 ccacgccggc ggcggcgacg ttcacgccaa tcgacgccgg gctgcggctg atcgacgtcg
     1081 acgacgtgtc ctttcgcggc tacggcggcc agcccatccg cggctggttg ttgcgcccgc
     1141 gcggggtgga tgggccgcgg ccgtgcgtgg tcgaatacat cggctatggc ggcgggcgcg
     1201 gccaccccca cgactggcta ctcttcgccg cggccggcta tgcccatttc gtcatggata
     1261 cgcgcggcca gggcagcgtc tggcggcgcg gcgacacggc cgacccggag ggcgacggcg
     1321 gcaacccaca gcatcccggc ttcatgaccc gcggcgtgct cagcccctct acctactact
     1381 acggccgcct gtacatcgac gccgtgcagg ccatcgccgc ggcgcgcgag catccggccg
     1441 tggacgccgg gcgcgtggcc gtctgcggcg gcagccaggg cgggggcgtg gccctggcgg
     1501 cggccgggct ggtgggcgat ctggccgcgg cgctcatcga cgtgcccttt ctgtgtcact
     1561 tccgccgggc gacgaccatc accgacgccg atccctatgg cgagatcgtc cgcttctgca
     1621 agatccaccg cgatcgcgtg gcgacggtct ttgcaacgtt ggattacttt gacggcatca
     1681 actttgccgc ccgcggccgc gccccggcgc ttttctccgt cgggctgatg gacgacgtct
     1741 gcccgccctc gaccgtcttt gccgcctaca accactatgc cggcccgaag gagatgcgcg
     1801 tctggccgtt caaccggcac gagggcggcg aggggtatca gacagccgag cggttgcggt
     1861 ttttgcggga gttgtggtag ccgtcagggc ctgagcaatc gacggccagg aagtggggtt
     1921 attgctggct tttgcctgtg gatattgtgc cgattgccgg cgggcgtgtc accattgatg
     1981 ggcagtcgtt tgaggtcagc gggccgcaag tgagcagcgc ggctgctctg ctcgcccggt
     2041 tatcggccgc cggcatcgat ccggcgactc tggcggcgcg ataggcgcgt tcgcgctcag
     2101 acggcgcggc cgacggccac caccgtcagc ccccacggca atcgccgtcg ccgcagccag
     2161 cgcgcctcca gcctcagcgg ctgcgtcaac agcgtgtcca gccagaccgg gttgggggcg
     2221 atgtcggagc gggcg
//
LOCUS       DAOSMQ010000514         2231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_48669_length_2231_cov_5.025276, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000514 DAOSMQ010000000
VERSION     DAOSMQ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48669_length_2231_cov_5.025276"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PK829_13560"
     CDS             <1..1043
                     /locus_tag="PK829_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42288.1"
                     /translation="PHWVPPLLTDMVTALNPAKHPFYEHSAAGFFVAARGDRVVGRIA
                     ALENVNYNRQHDSRTGQFYFFDCEDDDEAAAALFGAACEWARARGLDTVIGPKGLSAF
                     DGYGLLERGYEHRQMMNMMNYNYPYYTRLVEGQGFVKEVDFVSVFLPAAGFRLDERIH
                     RIAERARQRQSLRVLDLRSKRDLRHWAPAIGRAYNQAFVNNWEYVPLTEREIQFVLDQ
                     LLLIANPRLLKFIAHDDDVVGFALSFPDISAAMQRARGHLLPFGLLDMLRELRVARGL
                     AGNGAGILPQFHGLGGNALLYTEMERTVRDFGAEVYEMTQVAETAVQMRSDLQNIGGE
                     PYKNHRVYRKPL"
     gene            1229..>2231
                     /locus_tag="PK829_13565"
     CDS             1229..>2231
                     /locus_tag="PK829_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42289.1"
                     /translation="MTGAAPSAAAPDTRHWDRGATFDLVAMTALLLFSLVLTVYRLTL
                     PTDGWLATEPNDFDARGFIYQANLMGLPSDLRPGDHLLAVEGIPFNSDIVTASWRLRD
                     EWRAGNSLRYSVVRDGQALELDVPLTHWRLADFWRGTATAENLIWYVSSVLFVVVALL
                     AFWRRPDSRAARALWVLAAVELTVVQITTLVPAMASDSAFAEVALSINLLIFATFTVL
                     LPPALIRFALVFPEPIPPLARRPWLALVPYAVGLLVMVAFLTQQYVFGWLWTAASVLI
                     ALALLVYSALIRRDAVSRAQLRWGLGGTIIGLALFFVTYIPVFLDVPAAVTSVLDTLS
                     "
BASE COUNT          331 a          782 c          709 g          409 t
ORIGIN      
        1 gcccacattg ggtaccgccg ctgctgaccg acatggtcac ggccctgaac ccggccaagc
       61 accccttcta cgagcactcc gcggccggct tctttgtggc ggcgcgtggc gaccgtgtcg
      121 tcggccgcat cgccgcgctg gagaacgtca actacaaccg ccagcacgac agccgcaccg
      181 gccaattcta cttcttcgac tgcgaggacg acgacgaggc cgcggcggcc ctctttggcg
      241 cggcctgcga gtgggcgcgg gcgcgcgggc tggacacggt gattggcccc aagggcctga
      301 gcgctttcga cggctacggc ctgttggagc gcggctatga gcaccggcag atgatgaaca
      361 tgatgaacta caactacccc tactacaccc gcctggtgga ggggcaaggg ttcgtcaagg
      421 aggtcgattt tgtctccgtc ttcctgccgg cggccggctt ccggctggat gagcgcatcc
      481 atcgcatcgc cgagcgcgcg cggcagcggc agagcctgcg cgtgctcgac ttgcgctcca
      541 agcgcgacct gcgtcactgg gcgccggcca tcggccgggc ctacaaccag gccttcgtca
      601 acaactggga gtacgtcccc ctgaccgagc gcgaaatcca gttcgtcctc gaccaattgt
      661 tgctcatcgc caacccgcgg ctgctgaagt tcatcgccca cgacgacgac gtcgtcggct
      721 tcgcgcttag tttccccgac atctccgcgg ccatgcagcg ggcgcgcggc cacctgttgc
      781 ccttcggcct gcttgacatg ctgcgcgagt tgcgcgtggc cagggggctg gccggcaacg
      841 gcgcgggcat cctgccgcaa ttccacgggc tgggcggcaa cgccctgctg tacacggaaa
      901 tggagcgcac cgtccgcgac ttcggcgctg aggtctatga gatgacgcag gtagccgaga
      961 cggccgtgca gatgcgcagc gacctgcaga acatcggcgg cgagccgtac aagaaccacc
     1021 gcgtctatcg gaaaccgttg tagggggtgg ccagggcgtt caccagccat agcacggccc
     1081 gcggacgccg gcttcgaact cacatcttgt cgcacctgtt gcgcctctca attcggccgg
     1141 cgccattcga cactttcgta acgatcagga gacgatcgcc ggctatactc ccgatcatgg
     1201 tcactctggt aacgttcgag gtactcccat gaccggcgcc gcgccatcag ccgcggcgcc
     1261 agacacacgc cactgggatc gcggggcgac gttcgatctg gtggcgatga ccgccctgct
     1321 gctgttctcc ctggtgctaa ccgtctaccg cctcactttg cccaccgacg gctggttggc
     1381 gacggaaccc aacgacttcg acgcgcgggg gttcatctac caggctaacc tgatggggtt
     1441 gccttccgac ctgcggcccg gcgaccatct actggccgtg gagggcatcc ccttcaacag
     1501 cgacatcgtc accgcctcct ggcgcttgcg cgatgagtgg cgcgccggca atagcctgcg
     1561 ttactccgtc gtgcgcgacg gccaagcgct ggaactggac gtgcccctga cccactggcg
     1621 gctggccgac ttctggcgcg gtacggccac ggccgagaac ctcatctggt acgtgagcag
     1681 tgtgctcttc gtggtcgtgg ccctgctggc cttctggcga cggcccgaca gccgggcggc
     1741 gcgcgcccta tgggtgctgg ccgccgtcga gttgaccgtc gtccagatca cgaccctggt
     1801 gccggccatg gcgagcgaca gcgccttcgc cgaggtggcc ctgagtatta acctgctcat
     1861 cttcgccacg ttcaccgtct tgctgccgcc ggcgctcatc cgctttgccc tggtcttccc
     1921 ggaacccatt cccccgctgg cgcgccggcc gtggctggcc ctggtgccct acgcggtggg
     1981 gctgctggtg atggtggcct tcctgaccca gcagtacgtc ttcggctggc tatggacggc
     2041 ggccagcgtc ctcatcgccc tggccctgct ggtctacagc gcgctcatcc ggcgcgacgc
     2101 cgtcagccgc gcccaattgc gctgggggct gggcggaacc atcatcggtc tggcgctgtt
     2161 cttcgtcacc tacatccccg tcttcttgga cgtgcccgcg gcggtgacga gcgtgctcga
     2221 tactctgtcg g
//
LOCUS       DAOSMQ010000515         2224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_48883_length_2224_cov_4.030890, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000515 DAOSMQ010000000
VERSION     DAOSMQ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48883_length_2224_cov_4.030890"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PK829_13570"
     CDS             complement(<1..1317)
                     /locus_tag="PK829_13570"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU42290.1"
                     /translation="MDRALQRLERVLELEKQQGYQNKAVVGGIRQFAVFWVTQANEEA
                     SDEADRALAEQVSEVLMEYGRLSGVEARARAIDSLLASMERRRSRRPEAPPAPPPAPP
                     PARSVPPPKATAPAEASPRPAPAAVAPPVDEEPAEEEAVAPDAAIEPERPFVTADPLG
                     LTQPVTALKGVGQKIAERLNRIGPQTIWELLYLFPRRYDDYTLMKPISKLKYGEQVTV
                     IGTIWQTRTRKTRINQSLVECIVNDGTGTVQATWFNQPWLADQLPAGLQIVLSGKVEL
                     FLGRLVFNSPEWEPLELEPLRTRRIVPVYPLTEGLLASKMREIMQRTVREWAERAVAP
                     RIRELDREHRFDASILAQMADLGLLGVSVPSSYGGTGTDYISLGVASEELEYVDTSLR
                     VILSVHVGLNSLTLLAWGTEAQKQRYLVPQAQGKKIATFGLTEPSAG"
     gene            complement(1384..>2224)
                     /locus_tag="PK829_13575"
     CDS             complement(1384..>2224)
                     /locus_tag="PK829_13575"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42291.1"
                     /translation="GDESTIKVHVHVSDPGRPLSYGISLGHISDVVVENMQAQMEDII
                     QAAPPAAMAVVAPDVQPNQVAVVAVAAGAGLAGVFRSLGAAGVVNGGQTNNPSTEEIF
                     RAIDELPTDKIIVLPNNKNIILAAEAARDLSSKQVTVVPSRSAPQGIAALMSLNPDRD
                     LASNAEAMTRSLSLVKTGEITRATRSVQLDGVDVAEGALIGLADGKLCCSGTDELAVL
                     SDLLEHLEAANCGLVTLYYGATVTLAQAADVAEHVRARYPDLEVEVIEGGQPHYDYVL
                     GAE"
BASE COUNT          343 a          750 c          740 g          391 t
ORIGIN      
        1 gccggcggaa ggctcggtca ggccgaaggt ggcaatcttc tttccctggg cttgcggcac
       61 caggtagcgc tgcttctgcg cctcggtgcc ccacgccagc agggtcaggc tgttgaggcc
      121 gacgtgtacc gacaggatca cgcgcagcga cgtgtccacg tactccagtt cctcgctggc
      181 caccccgagg ctgatgtagt cggtcccggt gccgccgtag gacgacggca cagaaacgcc
      241 gagcagcccc aggtcggcca tctgcgcgag gatcgacgca tcgaagcggt gttcgcggtc
      301 cagctcgcgg atgcgcggtg ccaccgcccg ctcggcccac tcgcggacgg tacgttgcat
      361 gatctcgcgc atcttgctgg ccagcaaccc ttcggtcagc ggatacacgg gcacaatgcg
      421 ccgcgtgcgc aacggctcta gctccagcgg ctcccactcc ggcgaattga acacgagccg
      481 ccccaggaac agttccacct tgccgctgag gacgatctgc aagccagccg gcaattgatc
      541 ggccagccag ggctggttga accacgtcgc ctgcactgtg cccgtgccgt cgttgacaat
      601 gcactcgaca agggactggt tgatgcgcgt cttgcgggtg cgggtctgcc agatggtgcc
      661 gatgaccgtt acctgctcgc catacttcag cttgctgatc ggcttcatca gggtgtagtc
      721 gtcgtagcgg cgcgggaaaa ggtagagcag ctcccagatg gtctgcgggc cgatacggtt
      781 caaccgctcg gcgatcttct ggccgacgcc cttcagcgct gtcaccggct gggtcaggcc
      841 cagcgggtcg gcggtgacga atggccgctc cggctcaatg gctgcatccg gcgcgaccgc
      901 ctcctcctcg gccggctcct cgtccaccgg cggcgcgaca gcggccgggg ccgggcgggg
      961 cgaggcctcg gccggggcgg tggcttttgg cggcggcacg gaacgggctg gcggtggggc
     1021 cggcggtggg gccggcggag cctctggccg ccgggagcgc cgccgctcca tgctggccag
     1081 gagcgagtca atggccctgg cccgcgcctc gacgcccgac aaccggccat actccatcag
     1141 cacttccgat acctgctcgg ccaacgcgcg gtcggcttcg tcgctggcct cttcgttggc
     1201 ctgggtaacc cagaacacgg cgaactggcg gatgccgccg acgaccgcct tgttttgata
     1261 gccttgttgt ttctctaact caagcacgcg ctcaagtcgc tgcaatgcac gatccatgtt
     1321 tatttggtgc tcccgcccac cgtcgcctag gggcggttgg gcgaaaaatg cgcgtcggcc
     1381 cggctactcc gcgccgagga cgtagtcgta gtgcggctgt ccgccctcaa tgacttcgac
     1441 ctccaggtcg ggatagcgcg cgcggacgtg ttcggcgacg tcggccgcct gggccagcgt
     1501 cacggtggcc ccataataga gcgtcaccag cccgcagttg gctgcctcca gatgctccag
     1561 caaatcgctc aaaacggcca actcatccgt gccagaacaa cagagcttgc cgtcggccag
     1621 gccgatgagc gcgccctcgg ccacgtcgac gccgtccagt tgaacggagc gcgtcgcccg
     1681 agtgatctcg cccgtcttga ccagactcag ggagcgcgtc atcgcctcgg cgttgctggc
     1741 caagtcgcgg tcggggttca acgacatcag cgcggcgatg ccctggggcg cggagcggct
     1801 gggcacgacg gtgacctgct tgctgctcag gtcgcgcgcg gcctcggccg ccaggatgat
     1861 gttcttatta ttgggcaaga cgataatctt gtccgtcggc agctcatcaa tcgctcggaa
     1921 gatctcctcg gtgctggggt tgttggtctg gccgccgttc acaacgccgg ccgcgcccag
     1981 gctgcgaaaa acgccggcca accccgctcc ggcggcgacg gccacgacag ccacctggtt
     2041 gggctgaacg tcgggcgcaa ccacggccat agccgcgggc ggcgcggcct ggatgatgtc
     2101 ctccatctgc gcctgcatgt tttcgacgac gacatcggag atgtgcccca ggctgatacc
     2161 atagctcaat gggcggccgg ggtcgctgac gtggacgtgg accttgatgg tgctctcgtc
     2221 gccg
//
LOCUS       DAOSMQ010000516         2223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_48925_length_2223_cov_3.137454, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000516 DAOSMQ010000000
VERSION     DAOSMQ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48925_length_2223_cov_3.137454"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PK829_13580"
     CDS             complement(<1..281)
                     /locus_tag="PK829_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU42292.1"
                     /translation="MSMELTVAGGGLAGTEAAWQAAELGVSVRLYEMRPGQRTPAHET
                     AWLAELVCSNSLGSALPHKAPGLLKAELRGLGSVILDCAARTAVPAGSSL"
     gene            397..1617
                     /gene="trpB"
                     /locus_tag="PK829_13585"
     CDS             397..1617
                     /gene="trpB"
                     /locus_tag="PK829_13585"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOU42293.1"
                     /translation="MDPNTLQSSPTLPDAQGKFGPYGGQFVPETLMPALEELIAAYDE
                     ARHDPAFVAEFEGLLSSYVGRPSPISHARRLSAKLGGAQIYFKREDLNHTGAHKINNA
                     LGQALLVKRMGKRRVVAETGAGQHGVATATACALLGLEGIIYMGEVDIARQQPNVGRM
                     KLLGAAVRPVASGSRTLKDAINEAIRDWVTNVATTHYLLGSVLGPHPYPMMVRDFQSV
                     IGREARAQMLALCGRLPDAIVACVGGGSNAMGIFHAFREDESVDLIGVEAGGEGVDGG
                     RHAARFADPAAARVGVLHGTKSYVMQTADGQISETHSISAGLDYPSVGPEHAWLRDQE
                     RAYYTYATDAEALAAFHELSRTEGIIPALESAHAVAEALKRAPTMRPDQILLVNLSGR
                     GDKDLDTVLHLVSG"
     gene            complement(1634..>2223)
                     /locus_tag="PK829_13590"
     CDS             complement(1634..>2223)
                     /locus_tag="PK829_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42294.1"
                     /translation="DKRTIELLWAYRADRLEHVGFVTGPRHIDPFEVRAAEAALLEAG
                     LPAVPWLVAGTERSDAPDLARFGWVAGAAILPMAEEAWDVAPTPEEAAQLVRHADQSC
                     TNGPYLLPSRVKPNEAVQGDLVDLLNSEDPAVRLAPAARLIRQLREAEQWLAEALAWT
                     DRALALVARWTPTPNARLARAELAHRRERLLRKLT"
BASE COUNT          356 a          797 c          753 g          317 t
ORIGIN      
        1 agcgacgacc cggcgggcac ggccgtccgc gcggcgcagt cgaggataac tgagccgagg
       61 ccgcgcaact cggccttcag caggcccggc gctttgtggg gcagggcgga gccgagggag
      121 ttgctgcaca ccagttcggc cagccaggcc gtctcgtggg cgggtgtacg ttggcccggc
      181 cgcatctcat agagccgcac ggacacgccc aattcggccg cctgccaggc cgcctccgtc
      241 ccggccagcc ctccaccggc gacagtcagt tccatgctca tggattgatt ctaccacacg
      301 ccgcccgtgg cgcgattctc ccgaatcgcg tcgcctcacc gcgcagatcg tagcgcgatt
      361 ctcccgaatc gcaccgaatc gcgttacact ttggccatgg acccaaacac cctccagtca
      421 tctcccaccc tgcccgacgc ccagggcaag ttcggcccct atgggggcca gtttgtgccg
      481 gagacgctca tgccggcact ggaagaactg atcgccgcct atgacgaggc gcgccacgac
      541 ccggcctttg tggccgaatt cgaggggctg ctgagcagct acgtcggccg cccctcgccc
      601 atcagccacg cccgtcgcct gtcggccaag ctgggcgggg cacagatcta cttcaagcgc
      661 gaagacctca accacaccgg cgcgcacaag atcaataacg ccctcggcca ggctctgctg
      721 gtgaagcgca tgggcaagcg ccgcgtcgtg gccgagacgg gcgcgggcca gcacggcgtg
      781 gccacagcca ccgcctgcgc cctgctgggg ctggagggca tcatctacat gggcgaagtc
      841 gatattgccc ggcaacagcc caacgtcggc cgcatgaagc tgctgggggc ggcggtgcgc
      901 ccggtggcca gcggcagccg cacgctcaag gacgccatca acgaggccat tcgcgactgg
      961 gtgaccaacg tggccacgac ccactacctg ctcggctccg tcctggggcc gcacccctac
     1021 ccaatgatgg tgcgcgactt ccagagcgtc atcggccggg aggcgcgggc gcagatgctg
     1081 gccctgtgcg gccggctgcc cgatgccatc gtcgcttgcg tcggcggcgg cagcaacgcc
     1141 atgggcatct tccacgcctt ccgcgaggat gagtccgtcg atctgatcgg cgtggaggcc
     1201 ggcggcgagg gcgtagacgg cggccgccac gccgcccgct ttgctgaccc ggccgcggcg
     1261 cgcgttggcg tgctccacgg cacgaagagc tacgtgatgc agacggccga cggccagatc
     1321 agcgagaccc acagcatcag cgccgggctg gactacccca gcgtcggccc ggagcacgcc
     1381 tggctgcgcg accaggagcg ggcctactac acctacgcca ccgacgccga ggccctggcc
     1441 gccttccacg agctatcgcg gacggagggc atcatcccgg cgctggaatc ggcccacgcc
     1501 gtggccgagg cgctcaagcg cgcgccaacg atgcggccgg atcagatttt gctggtgaat
     1561 ttgagtgggc ggggggataa ggatttggat acggtgctgc acctggtgtc gggctaggcc
     1621 gagtcccgct cggtcaggtc agcttgcgca gcaaccgctc ccgccggtgg gccaactccg
     1681 cccgcgccag ccgcgcgttg ggcgtgggcg tccagcgggc aacgagggcc agggcgcggt
     1741 cggtccaggc cagcgcctcg gccagccatt gctccgcctc gcgcaactgc cgaatcagcc
     1801 gggccgcggg ggcaagccgg acagccgggt cctccgagtt cagcaggtcg acgagatcgc
     1861 cctgaacggc ctcgttcggc ttgacgcgcg acggcaacag gtaggggccg ttcgtgcaag
     1921 actggtcggc atggcggaca agctgcgcgg cctcctctgg ggtaggcgcc acgtcccacg
     1981 cctcctcggc catcggcagt attgctgcgc cggcgaccca cccgaaacga gccaggtcag
     2041 gcgcgtcgct gcgctcagtg cctgccacca gccacgggac ggcgggcagt cctgcctcga
     2101 ggagcgcggc ctccgccgcg cgcacctcga acgggtcgat gtgacggggt cccgtgacga
     2161 agccgacgtg ctccagccga tcagcacggt atgcccacag cagctcaatg gttcgcttgt
     2221 ctg
//
LOCUS       DAOSMQ010000517         2219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_49057_length_2219_cov_3.557763, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000517 DAOSMQ010000000
VERSION     DAOSMQ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49057_length_2219_cov_3.557763"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK829_13595"
     CDS             <1..391
                     /locus_tag="PK829_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOU42295.1"
                     /translation="YPSAWSISRRALADDVIGGYDIPAGAIVAMSPYTIHRRPDFWPD
                     PERFDPDRFTPERAAGRPRFAYFPFGGGARQCIGNYFALMESLIIIPTIVQHFRLSPC
                     WSEPIEGHALVTLRPRGGVPVAVEKRR"
     gene            388..1371
                     /locus_tag="PK829_13600"
     CDS             388..1371
                     /locus_tag="PK829_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU42296.1"
                     /translation="MKVLFIGGSGIISSACTALAIDEGIDLYLLNRGQTGLRPIPAAA
                     QVLHADIRQPASAAAALAGHTFDAVVDWIAFTTDHVAVDLELFRGRVGQFIFISSASA
                     YQKPPQRLPITEDTPLDNPFWQYSRNKAACEAMLTAAHRDEGFPATIVRPSHTYDATL
                     LPFHGGYTTLDRLRRGAPLLIHDDGESLWTLTHHRDFARAFVPLLGESRAIGEAYHIT
                     SDEVLTWNEIAGLLAAAAGVAADLVHVPSTTIAGYDQGLGDSLLGDKAHNTYFDNSKI
                     RTIVPGFAAEIPFADGAREIVAWYDAHPEAQRVDPSYNALLDRIIADAREK"
     gene            1496..2059
                     /locus_tag="PK829_13605"
     CDS             1496..2059
                     /locus_tag="PK829_13605"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HOU42297.1"
                     /translation="MNPFHWLFDKAYPAIRFLYERVQGNRWFDRITPQIWLGGAPTYA
                     RDYQFLLDNGITAVLDMRAEREGDRAFYANHDIAYKRLAVLDATVPGDDYLSEGADWI
                     DAQIDSGRTVLVHCAKGRGRSATLVAAYLMRHQDLPYDDCQTMMKEARPLVKLEDRHR
                     RRLEQWHAEPWHAEPRPIEGTDQDATT"
BASE COUNT          365 a          808 c          658 g          388 t
ORIGIN      
        1 ttatccctcc gcctggtcga tcagccgccg ggcgttggcc gatgacgtga tcggcggcta
       61 cgacatcccc gccggcgcaa ttgtggccat gagtccctat accatccacc gtcgcccgga
      121 cttctggccc gacccggagc gcttcgatcc cgaccggttt acgccggagc gggccgcggg
      181 ccggccgcgg ttcgcctact tcccctttgg cggcggcgcg cggcagtgca tcggcaacta
      241 ctttgctctc atggaaagcc tgattatcat ccccacaatc gtccagcact tccggctcag
      301 cccctgctgg tcagagccaa tcgaggggca tgctctggtg acattacgac cgcgtggggg
      361 cgtccccgtg gcggtggaga agcgacgatg aaagttctgt ttattggcgg cagcggcatc
      421 atcagttcgg cctgcacagc gctggccatc gacgaaggta ttgacctcta tctgctcaat
      481 cgcggccaga cgggcttgcg gcccatcccg gcggcggcgc aggtgttaca cgccgacatc
      541 cgccagccag cctcggccgc cgcggccctc gccggccaca cctttgacgc cgttgtcgac
      601 tggattgcct tcacgaccga ccacgttgcc gtcgatctgg aactctttcg cggtcgggtg
      661 gggcaattca tcttcatcag ttcggcttcg gcctatcaga agccgcccca gcgcctgccc
      721 atcaccgagg acacgccgct ggacaatccc ttctggcaat actcgcgcaa caaggccgcc
      781 tgcgaggcga tgttgaccgc ggcccaccgc gatgagggct tcccggcgac catcgtccgg
      841 ccgtcgcaca cctacgacgc cacgctgctg cccttccacg gcggctacac gacgctcgac
      901 cggctgcggc ggggcgcgcc cctcctgatc cacgacgacg gcgaatcgct gtggacgctg
      961 actcatcacc gcgacttcgc ccgcgccttt gtgcccctgt tgggcgagtc gcgggccatt
     1021 ggcgaggcct accacatcac ctctgacgaa gtgctgacct ggaacgagat cgccgggctg
     1081 ctggccgcgg ccgccggcgt ggccgccgac cttgtccacg ttccctcgac gaccatcgcc
     1141 ggctacgacc agggcctggg cgatagcctc ctgggcgaca aggcccacaa cacctatttc
     1201 gacaacagca aaattcgcac cattgttccc ggcttcgccg ccgagattcc ctttgccgac
     1261 ggggcgcgcg agattgtcgc ctggtacgac gcccaccccg aggcgcagcg cgttgacccc
     1321 tcctacaacg ccttgctcga ccgcattatt gccgacgccc gcgagaaatg atggccagcg
     1381 gccagacctg agaggccttc cgagaccttt tcattccgac tgatgatccc cgaccagcgc
     1441 cctggccgct cccctacgtt gccaagccgg cccggcggcg ttatgcttac gggcgatgaa
     1501 tccgtttcac tggctattcg acaaggccta tccggccatc cgcttcctct atgagcgcgt
     1561 gcaaggtaac cgctggttcg accgcatcac gccgcaaatc tggctgggcg gcgcgccgac
     1621 ctacgcacgc gactaccagt tcctgctcga caacggcatc accgccgtgc tggacatgcg
     1681 cgctgagcgc gaaggcgacc gcgcctttta tgccaaccac gacatcgcct acaagcggct
     1741 ggccgtgctc gacgccaccg tccctggcga cgactacctg agcgaggggg ccgactggat
     1801 cgacgcgcag atcgactccg ggcgtaccgt cctggtacac tgtgccaagg ggcgcggccg
     1861 ttcggccacg ctggtagccg cctatctgat gcgccatcag gacttgccct atgacgactg
     1921 ccagacgatg atgaaagagg cccggccgct ggtcaagctg gaggaccgcc accgccgccg
     1981 cctggagcag tggcacgccg agccgtggca cgccgagccg aggcccatcg aaggaacgga
     2041 tcaggacgcg acgacatgat tgtccccgct gccacgccgc ccgcctcgct cgctgtcgcc
     2101 cagcgttcgc tgggggaacc ctcccgccaa tttccgctct ttccgccgct ggtggagggc
     2161 tgcccaacca ccagcacggc tgagttgcaa tatccgctgg cgctcgacta cgattatgc
//
LOCUS       DAOSMQ010000518         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_49117_length_2217_cov_4.339500, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000518 DAOSMQ010000000
VERSION     DAOSMQ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49117_length_2217_cov_4.339500"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK829_13610"
     CDS             complement(<1..181)
                     /locus_tag="PK829_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42298.1"
                     /translation="MSLPLTAVAFGRPGVGPDGNAHRGKPARNHGNGDYHGVAHQHRD
                     IDARRPRPGHRDPAVS"
     gene            539..820
                     /locus_tag="PK829_13615"
     CDS             539..820
                     /locus_tag="PK829_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42299.1"
                     /translation="MVDQKKQEALAALFRETISAHHDAYIEADGADADWPIWYAGYLR
                     DRLAELLPADFTKSELVYLLITLDRELKTRAPGAEWVGYYTRWFMERYG"
     gene            844..1488
                     /locus_tag="PK829_13620"
     CDS             844..1488
                     /locus_tag="PK829_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HOU42300.1"
                     /translation="MFDQRERRELEKRMKEENRLPPGQSATIKFPVLHYGPVPRVDLA
                     KWDFRVFGLVEEDRRWTWAEFNELPRTQVVLDIHCVTRWSKFDTVWEGVSVKTLVERG
                     LIRLKPEARYVVQHCEVGYTTNTPLEFVLQDGFIMATHFDGQPLAPDHGYPLRGVAGV
                     VGGRRADDKYFWKGGKWLRGLEFRADDQPGFWERNGYHNVADIWKEQRHGWPGF"
     gene            1525..>2217
                     /locus_tag="PK829_13625"
     CDS             1525..>2217
                     /locus_tag="PK829_13625"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU42301.1"
                     /translation="MASYCPHCGAELVRAGAAFCATCGRPLQPRSGAIGSGSTGPRLV
                     AQLPGRPEQTFVVARPEITIGRDPDNDFILDNPVVSRHHATLYLRDGVYWLRDLGSTN
                     GTAVNGQRITETALRDGDILRIGDEQGNNVGLVFRHAGAAPSPGTIQLGRRALGQGAA
                     LVIGRDPSANLTLDHPTVSRLHAEVRLTPTGPMLRDLGSSNGTFLNGELISSPRPLSP
                     RDVVQIGPYKLVY"
BASE COUNT          388 a          708 c          745 g          376 t
ORIGIN      
        1 aggagacggc cgggtcgcgg tggccggggc gggggcgcct ggcgtcgatg tcgcggtgct
       61 ggtgggcgac gccgtggtag tcgccgttcc cgtggttgcg ggcgggcttg ccccggtggg
      121 cgttgccgtc tgggccaaca ccgggccggc caaaagcaac agcagtaagg ggcagagaca
      181 tgccagacgc agactatagg cggatactcg gttcatctcc gtcccactct ctctcctgtc
      241 cgtaatggcg ggcacactga gggggcattg tagcccattt ttcgtgcgat gctagtggcc
      301 catgtccgta ggaccttgcc aaagttgtct agcgaccatt gacctgacag gtgggcggcc
      361 gagaaggttt ggccattcac ctgtcaggtc ggcgcgcggc ggccggcaac tgcaaaactc
      421 cgcgccggcg gcgcaagaag ttgacggaca tagcccggta ggcgtatgat ctccacatgg
      481 ccagacaaga ggcccgtggc tggtctccag ccgccatttg accgatgcag gaggctacgt
      541 ggtcgatcag aagaaacagg aagcgctcgc ggctctcttc cgcgagacca tcagcgccca
      601 tcacgacgcc tacatcgagg cagatggggc cgacgccgat tggcccatct ggtatgccgg
      661 gtatctgcgc gaccggttgg cagaattgct gccagccgac ttcaccaaaa gcgagctggt
      721 ctatttgctc atcaccctcg accgcgagtt gaagacccgc gcgcccggcg cagagtgggt
      781 gggctattac acccgttggt tcatggagcg ttacggctga gcggggagca caaggggcta
      841 atcatgttcg atcagagaga acgacgcgag ttggaaaagc gcatgaagga ggagaaccgg
      901 ctgccccctg gccagtcggc gacaatcaaa ttccccgtgc tccactatgg ccctgtgccg
      961 cgggtcgatc tggccaagtg ggacttccgc gtctttggcc tggtggagga ggaccggcgc
     1021 tggacgtggg cggagttcaa tgagctgccg cgcacgcagg tcgtgctcga cattcattgc
     1081 gtcacgcgct ggtcgaaatt cgacaccgtc tgggagggag tgtcggtcaa gacactggtc
     1141 gagcgcggtc taatccgtct caagccggag gcgcgctatg tcgtccagca ttgcgaagtt
     1201 ggctacacga ccaacacgcc gctggagttc gtgctacaag atggcttcat catggcgacc
     1261 catttcgatg gccagccgct ggcgcccgac cacggctacc cgctgcgcgg cgtggccggc
     1321 gtcgtcgggg gccggcgggc cgacgacaag tacttctgga agggcggcaa gtggctgcgc
     1381 gggctagagt tccgcgccga cgaccagcct ggcttctggg agcgcaacgg ctatcacaac
     1441 gtcgccgaca tctggaaaga gcaacgccac ggttggccgg gcttctgata gcggccgcag
     1501 agcgcgcaga cagggagggg tggcttggcc agttattgtc cccattgtgg cgcggagctt
     1561 gtccgcgcgg gggcggcgtt ttgcgccacg tgcggccggc cgttgcagcc gcgttccggg
     1621 gccattggct ccggcagcac tggcccgcga ctggtggcgc agttgcccgg ccgcccggag
     1681 cagacgttcg tcgtcgccag gccggagatt accatcggcc gcgaccccga caacgatttc
     1741 atcctcgata accccgtcgt ctcgcgccac catgccacgc tgtacctgcg cgacggcgtt
     1801 tactggctcc gcgacctggg cagcaccaac ggcacagcgg tcaacggcca acgcatcacc
     1861 gaaacagcgc tgcgcgacgg cgatatcctt cgcattggcg acgagcaggg caacaacgtc
     1921 ggtctggtct tccgccacgc cggggccgcc ccctcaccgg gtacgatcca gctcggccgg
     1981 cgggcgctgg ggcaaggggc tgcgctggtc atcggccgcg acccatcggc caacctgaca
     2041 ctcgaccatc ccaccgtctc gcgactgcac gccgaagtgc gcctgactcc gaccgggccg
     2101 atgctgcgcg acctgggcag cagcaacggc actttcctca acggcgagct tatcagcagc
     2161 ccgcgaccgc tgtcgccgcg cgacgtagtg cagatcggcc cctacaagct ggtctac
//
LOCUS       DAOSMQ010000519         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_49127_length_2217_cov_3.087419, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000519 DAOSMQ010000000
VERSION     DAOSMQ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49127_length_2217_cov_3.087419"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PK829_13630"
     CDS             <1..983
                     /locus_tag="PK829_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42302.1"
                     /translation="PAPQHSSPRPLRPPAPLPPQAPASVKVHPDIALAILRAKQAAPG
                     RVWLLLRHADAAGRGAIDERVARQLLSGPDAPLRVCGRRQLANLLAAGDGLFWQRAAG
                     VIWLRSVVRVAAALGVARLGGVPVAVPVAALTGPIGRARAHLYASFHSGRQSVDLLTG
                     APRPRGPIARATLTALSAAAPNSQRAYERRAGVGRRSAVAIGPPAGAVDEHEVAWRRG
                     RALFHLRDRKGRFGRPGAVYLAWQLPNEYTGPHATLPRGRQKRLNRALADLFLDGMTG
                     NGEQPGGATGRPRRRFYGSARPAALARHNGERYWRGRGGEWYWQPETDAR"
     gene            1053..1964
                     /locus_tag="PK829_13635"
     CDS             1053..1964
                     /locus_tag="PK829_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU42303.1"
                     /translation="MKLHILDTHFQGMAQVTAVYLVVGPEGPVLVESGPGSTLQAVLK
                     ALDGHGFGPADIRHVLLTHIHLDHAGAAGWWAQQGAQLYVHHVGAPHLIDPGKLLASA
                     GRIYGARMDTLWGPVLPAPAERVTLLYDGDRIHAGGLTFTALDTPGHAYHHHTYRLRD
                     YREGELAFTGDAAGVNLPLVNVVDLPAPPPEFHMETWLRTLDRLDAAGLDTIYPTHFG
                     PVRDVHGHLVALRGLMVAAVAFVAERAAAGVARDALLADYIAWNRQRARAANLSAAAI
                     DRYQAANPLFMSVDGILRYLGKRGDRG"
BASE COUNT          298 a          894 c          729 g          296 t
ORIGIN      
        1 ccccagcccc ccagcattct tccccccggc ccctccgccc cccagccccc ctgcctccac
       61 aggcccccgc gtctgtcaaa gtccaccccg acatcgccct ggccatcctg cgggccaagc
      121 aggccgcccc cggccgcgtc tggctgctgt tgcgccacgc cgacgccgcc ggccgcgggg
      181 ccatcgacga gcgggtcgcc cggcaactgc tgagcggccc cgacgcgccc ctgcgcgtct
      241 gcggccgccg ccagctggcc aacctgctgg ccgcgggcga cgggctgttc tggcagcggg
      301 cggcgggcgt catctggctg cggtcggtgg tgcgcgtggc cgcggcgctg ggcgtggccc
      361 ggctgggcgg cgtgcccgtg gccgtgcccg tggccgcgct gaccggcccc atcggccgcg
      421 cccgcgccca cctctacgcc agcttccaca gcggtcggca gagcgtggat ctgctgaccg
      481 gcgcgccccg cccgcgcggc cccatcgccc gcgccaccct gaccgccctc agcgccgccg
      541 cgcccaacag ccagcgcgcc tatgagcgcc gcgccggcgt cggccgccgc tcggccgtgg
      601 ccatcggccc gcccgccggc gcggtcgatg agcacgaagt tgcctggcgg cgcggccggg
      661 cgctgttcca cctgcgcgac cgcaaaggcc gcttcggccg ccccggcgcg gtctacctgg
      721 cctggcaact acccaacgaa tacaccggcc cgcacgcgac gctgccgcgc ggccgccaga
      781 agcgcctcaa tcgggcgctc gccgacctgt tccttgacgg gatgacgggc aacggcgagc
      841 aacccggcgg cgcgactggc cggccgcggc ggcgtttcta cgggtcggcc agaccggccg
      901 ctctggcccg ccacaacggc gaacgctact ggcgcggccg gggtggcgaa tggtactggc
      961 aaccggagac cgatgcgcgc tgagcgcccg cggccggcca accctggcaa taacttgcat
     1021 ccttgatcgg gataaagcta tgatcctctg gcatgaagct ccacatcctc gacacccact
     1081 tccagggcat ggcccaggtc accgcggtct atctcgtcgt cggtcccgaa gggccggtgc
     1141 tggtcgaaag cggccccggc agcaccctgc aggccgtcct gaaggccctc gacggccacg
     1201 gcttcggccc ggccgacatt cgccacgtcc tactgaccca catccacctc gaccacgccg
     1261 gagcggccgg ctggtgggcg cagcaggggg cgcagctcta cgtccaccac gtcggcgcgc
     1321 cgcacctgat cgaccccggc aagctgctgg ccagcgccgg gcgcatctac ggcgcgcgca
     1381 tggacacgct ctgggggccg gtgctgcccg cgccggccga gcgggtgacg ctgctctatg
     1441 acggcgaccg catccatgcc ggcggcctga ccttcaccgc cctggacacg cccggccacg
     1501 cctaccacca ccacacctac cgcctgcgcg actaccgcga gggcgagctg gccttcactg
     1561 gcgacgccgc cggcgtcaac ctgcccctgg tcaacgtcgt cgatctgccc gcgccgccgc
     1621 cggagttcca catggagacg tggctgcgca cgctcgaccg gctggacgcg gccggcctgg
     1681 acaccatcta cccgacccac ttcggccccg tccgcgacgt ccacggccat ctggtggccc
     1741 tgcgcgggct gatggtcgcg gccgtcgcct tcgtggccga gcgcgcggcc gccggggtgg
     1801 cacgcgacgc gctgctggcc gactacatcg cctggaaccg ccaacgcgcc cgcgccgcca
     1861 acctctccgc cgcggccatc gaccgctacc aggccgccaa cccgctgttc atgtcggtcg
     1921 atgggatttt gcgctatctg gggaagaggg gggatagggg ctagggaaga ggttccaggg
     1981 gccaggggcc aggggccagg gaagagcgcg cttgccctgg tccgtagttg agtagcccgg
     2041 ttgcctttgg gccagagcag cctaggcgac ccggtcgaat ggcccgcatt acgaatctgc
     2101 atgccagcca ccaggcaaca acgttcttcc cctggcccct ctcccctagc cttcaggccc
     2161 ggtcgaacgg cccgcattac gaatctgcat gccagccacc aggcaacaac gttcttc
//
LOCUS       DAOSMQ010000520         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_49429_length_2206_cov_4.190609, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000520 DAOSMQ010000000
VERSION     DAOSMQ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49429_length_2206_cov_4.190609"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PK829_13640"
     CDS             <1..797
                     /locus_tag="PK829_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU42304.1"
                     /translation="VVIGYMGNNVYPFRAGEVLRAVVLRQREGVAVSSSLATIVVERV
                     FDGLVMLLFVFVALPFAPLPSDSIRFVVVLGSVLFFGALLVFLAVAVVPARFLRLTEW
                     LVGRFVPQRLRAPLLSFAERFVEGLLSLRDPRNLIMIFLTSVVIWLLETVKYWFVMHA
                     FDFTVSFFALMLMNGIVNLATTLPSAPGYIGTFDGPGIAVLTLYGVDPAVATAYTLTL
                     HAALWLPITLLGIYYMIRTQLHWADIGRAVEQGRALDEAAEGEAAR"
     gene            794..2143
                     /locus_tag="PK829_13645"
     CDS             794..2143
                     /locus_tag="PK829_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU42305.1"
                     /translation="MKIAIVGAGIAGLSAAYDLLRAGHTVTLLEGDSQTGGLATGFRD
                     ERWEWPLEKFYHHLFTSDRAIIGLLDEIGFRDKLFFPRPTTSVIYEDRIVPFDSPLRW
                     ITFPGFNPIDVARFGAVSAYLRFTEPWRELEKHTADSWLRRAYGDKIYETTWRPMLIG
                     KFGPYYQEVNMAWMWARLHVRSPRLGYFEGGFQAFVDRLTGVVRGLGGDIRLNCPAES
                     IQSDAGGLLIRAGGQTEGYDAGIVTTSPALLARLAPDLAGDYLGQILALKSMGAVVMT
                     LALKHSLMAESHTYWLNIPATSADKAGGVPFLALVEHTNYIDRRHYGGDHILYCGDYV
                     TPDHPYFTMSQAELERLFVGVLHRFNPDFRPDWVRQSWLFRARYAQPVPGLNHSKAIP
                     DLRTPIPGLYLASMSQVYPWDRGTNYAVEIGRRVARTLLDDGPSLLGRANVARSSPA"
BASE COUNT          323 a          752 c          685 g          446 t
ORIGIN      
        1 tcgtcgtcat cggctacatg ggcaacaacg tctacccctt ccgcgccggc gaggtattgc
       61 gcgccgtcgt tttgcgccag cgcgagggcg tcgccgttag cagcagtctg gcgaccatcg
      121 tcgtcgagcg cgtcttcgat gggctggtga tgctgctgtt cgtcttcgtg gccctgccgt
      181 tcgccccgct gcccagcgac agcatccgct ttgtcgtcgt tttgggcagc gtgctgttct
      241 tcggcgcgct gctggttttc ctggccgttg ccgtcgtgcc ggcgcgcttc ctgcgcctga
      301 cggaatggct cgtggggcgc ttcgttcccc agcggctgcg cgcgccgctg ctgagcttcg
      361 ccgagcgctt tgtcgagggg ctgctcagcc tgcgcgaccc gcgcaacctg atcatgatct
      421 ttctgacctc tgtcgtcatc tggttgctgg agacggtcaa gtactggttc gttatgcacg
      481 cctttgactt caccgtgtcc ttctttgccc tgatgctcat gaatggcatt gtcaacctgg
      541 ccacgacgct gccctcggct cccggctaca tcggcacatt cgacgggccg ggcattgctg
      601 tgctgacgct ctatggcgtt gatccggcgg tggccacggc ttacacgctg acgcttcacg
      661 ccgcgctgtg gctgcccatc accctgctgg gcatctacta catgatccgt acccaactcc
      721 attgggccga tatcggccgc gccgtcgagc aaggccgcgc tctcgatgaa gcggcggagg
      781 gggaggcggc gcgatgaaga tcgccatcgt tggcgcgggc attgccggcc tgtcggccgc
      841 ctatgacctc ctccgcgctg ggcacactgt taccctgctg gagggcgaca gccagaccgg
      901 cggcctggcc accggcttcc gcgatgagcg ctgggagtgg ccgctggaga agttctacca
      961 tcatctgttc acctccgacc gggccatcat cggcctgctg gacgaaatcg gctttcgcga
     1021 caaactcttt ttcccgcggc cgacgacctc tgtgatctac gaggatcgca tcgtcccctt
     1081 cgattcgccc ctgcgctgga tcacctttcc cggcttcaac ccgatcgacg tcgcccgctt
     1141 tggggccgtc agcgcctatc tgcgcttcac cgagccgtgg cgcgaattgg agaagcacac
     1201 cgccgatagc tggctgcggc gcgcctacgg cgacaagatt tacgagacga cgtggcggcc
     1261 gatgctcatt ggcaagttcg ggccatacta tcaggaagtc aacatggcct ggatgtgggc
     1321 gcgcctccac gtgcgcagcc cgcggctggg ctacttcgag ggcggcttcc aggccttcgt
     1381 cgaccggctg accggggtgg tgcgcggcct gggcggcgac atccgcctga attgcccggc
     1441 cgagtctatc cagtccgacg ccggcggcct gctgataagg gccggcggcc agacggaggg
     1501 ctacgacgcc ggcatcgtca ccacgtcgcc ggcgctgctg gcccggctgg cccccgacct
     1561 ggccggcgac tacctgggcc aaattctggc cctgaagagc atgggcgcgg tggtgatgac
     1621 cctggctctg aagcactcgc ttatggccga aagccacacc tactggctca acatcccggc
     1681 cacttcggcc gataaggccg gtggcgtgcc cttcctggcc ctggtcgaac acaccaacta
     1741 catcgatcgc cgccactacg gcggcgacca tatcctctat tgcggcgatt acgtgacgcc
     1801 agaccatccc tacttcacga tgagccaggc ggaactggag cgcctgttcg tcggcgtgtt
     1861 gcatcgcttt aaccctgact tccggcccga ttgggtgcgc cagagctggc tcttccgcgc
     1921 ccgctacgcc caacccgtgc ccggtctgaa ccactcgaag gccatccccg acctgcgcac
     1981 gcccatcccg ggcctctatc tggccagcat gagccaggtc tacccctggg accggggcac
     2041 caactacgcc gtcgagatcg gtcggcgggt agcgcggacg ctgctcgatg acggcccgtc
     2101 gttgttgggc agggcaaacg ttgcccgatc atcgcccgcc tgacttggta agaggttgga
     2161 cagacgctag aataagcttc tagttttgta ctggcgccgc gcaggg
//
LOCUS       DAOSMQ010000521         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_49488_length_2204_cov_3.919497, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000521 DAOSMQ010000000
VERSION     DAOSMQ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49488_length_2204_cov_3.919497"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PK829_13650"
     CDS             <1..442
                     /locus_tag="PK829_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HOU42306.1"
                     /translation="RVVTVMELLSPANKLYAKGREEYIQKRYEITGSRTNLVEIDLLR
                     AGQPMPLQGRRVDSDYRILVSRATTRPRALLYPFNLRDPIPTFDLPLLPGDEEPAVDL
                     GAIFHALYERARYDLSLDYSQAPVPPLNDADIPWAQEQIAQRQS"
     gene            599..1252
                     /locus_tag="PK829_13655"
     CDS             599..1252
                     /locus_tag="PK829_13655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU42307.1"
                     /translation="MPAKDSDDRWAEYYQKVADRAPRDLFRRLMALVEASAPNAPRGQ
                     AIDLGCGDGTETLALLAAGWRVLAIDGSPAAIRLVAERARPSDASRLQTQLAAFDEVI
                     LPSADLLYAGLSLPFCPPAEFGALWHKIRAALRPGAWLAAHFFGVRDDWASNPTMSFH
                     TAEALRVLLAGLEIAHWEEVEDDRASAFEAMKHFHYFEVSAWLRSDSGESRHGGRGH"
     gene            1482..1796
                     /locus_tag="PK829_13660"
     CDS             1482..1796
                     /locus_tag="PK829_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42308.1"
                     /translation="MTHQPINRYDHFAALGRAGAAHDVDALMHALLAHDDLATSRLVD
                     FAIGLVDTREGAARLRHYLFDGAPIQRNYAALYFKRRGRVGLLEEAVAQGKIDAAQAF
                     SR"
     gene            1901..>2204
                     /locus_tag="PK829_13665"
     CDS             1901..>2204
                     /locus_tag="PK829_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU42309.1"
                     /translation="MTDPDPHASILAHNRIAWDKWAQDGDRWTLPVSPAEVAAARAGE
                     WVIYLTEEKPTPRDWFPADLRGVDVLCLASGGGQQGPILAAAGANVTVFDNSPEQLA"
BASE COUNT          373 a          780 c          703 g          348 t
ORIGIN      
        1 gcgcgtcgtc acggtcatgg aactcctttc cccggccaac aagctctatg cgaaagggcg
       61 cgaggagtac atccagaaac gctacgagat caccggcagc cgcaccaacc tggtggagat
      121 agacctcctg cgcgccggac agccgatgcc gctacagggg cggcgcgtgg acagcgacta
      181 tcgcatcctc gtcagccggg ccacgacccg gccgcgcgcg ctgctctacc cattcaacct
      241 gcgcgacccc attcccactt ttgacctgcc ccttctgcct ggcgacgagg agccggcggt
      301 cgatctgggg gccatcttcc acgccctcta tgagcgcgcg cgctatgacc tgagcctcga
      361 ctactctcaa gcccctgtgc ccccactgaa tgatgccgac attccatggg cgcaggagca
      421 gatcgcgcaa cggcaatcgt aggcccaccc cagattcatg agactcattc agagaatccg
      481 gtgcgacggc gatagccatt ggccgcaaca acaagttgcc agccggttgg atgcgccaac
      541 ccaaacctac cgcgctcgcc agtgagcgcg ggcgacgacc acccgatcga ggaacccgat
      601 gcctgccaag gactcagacg accggtgggc cgagtactat cagaaagtcg ccgaccgcgc
      661 gccgcgcgac ctgttccggc ggctcatggc gctggtcgaa gccagcgcgc ccaacgctcc
      721 acgcggccag gccattgacc tcggctgtgg cgacggcacg gagacgctgg cgctcctggc
      781 cgcgggctgg cgcgtgctgg ccattgatgg ctcgcccgcg gccatccggc ttgtggccga
      841 gagggcgcgt ccgtccgacg ccagccgctt gcagacgcag ctcgccgcct tcgacgaggt
      901 catcctgccc tcggccgacc tgctctacgc cgggctaagc ctgcccttct gcccgccggc
      961 ggagtttggc gccctgtggc acaagattcg cgcggcgttg cggccgggag cgtggctggc
     1021 ggcgcacttc ttcggcgtcc gcgatgactg ggcgagcaac ccgacgatga gcttccacac
     1081 ggccgaggcg ctgcgcgtcc tgctggccgg gctggaaatc gcccactggg aggaagtcga
     1141 ggacgatcgc gcctctgctt tcgaggcgat gaagcacttc cactattttg aggtcagcgc
     1201 ctggctgcgg agcgattcgg gagaatcgcg ccacggtggg cgcggccatt gacatacccc
     1261 tcccccagtg ctactatccc cgccatgcaa tacattctgt cggccagcag tgattgtcgg
     1321 aacgattcgc ccaaaccccg cgcggtcagg cggatggccg atgcctgctg atttacacga
     1381 caggtaacga caccggaaag ccaacaggcg cggccacccc cgcgcctttt ttgttggccg
     1441 cgcgccggag ccaggggttg ccgcaccaga aaggagacgc gatgacccac caaccgatca
     1501 accgctacga ccacttcgcc gcgctcggcc gcgctggcgc ggcccacgac gtggacgcgc
     1561 tgatgcacgc cctgctggcc cacgacgacc tggccacatc gcggctggtt gacttcgcca
     1621 tcggtctggt ggacacgcgc gagggcgcgg cgcggctgcg ccactacctg ttcgacggcg
     1681 cgcccatcca gcgcaactac gccgcgctgt acttcaagcg gcgcgggcgc gtgggcctgc
     1741 tggaggaagc cgtcgcccag ggtaagatcg acgcggcgca ggccttctcg cgctaggccg
     1801 gcggccgtgt ctgacatggc cgcggacagt ttgcggtcgc tcttggtagc ttatggtggc
     1861 ccttttatac tacagtaacg actgttgcaa gaggcaaagc atgaccgatc ccgatcccca
     1921 cgccagcatc ctggcccaca accgcatcgc ctgggacaag tgggcccagg acggcgaccg
     1981 ctggacattg cccgtctcgc cggccgaggt ggccgcggcc cgcgccggcg agtgggttat
     2041 ctatctgacc gaggagaagc ccacgccgcg cgactggttt ccggccgacc tgcgcggggt
     2101 cgacgtactc tgtctggcct ccggcggcgg gcagcagggg ccaattctgg ccgcggccgg
     2161 ggccaacgtg accgtcttcg acaattcgcc ggagcaactc gccc
//
LOCUS       DAOSMQ010000522         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_49780_length_2195_cov_3.992991, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000522 DAOSMQ010000000
VERSION     DAOSMQ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49780_length_2195_cov_3.992991"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /gene="purH"
                     /locus_tag="PK829_13670"
     CDS             <1..757
                     /gene="purH"
                     /locus_tag="PK829_13670"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOU42310.1"
                     /translation="VIVKHLSPCGLAQADSVAAALPAAIAADPVSAFGGVIAANQPVG
                     ADFVAALAAAELFVEAVIAPAFDEEALAWFAAHKKNCRLVARGGALERAGGAALEWRA
                     IRGGLLAQTGDRGDPPNVVWRVVSRRAPTDEEMAALRFAWLACQHVKSNAIVLARAEG
                     AAAAVVGIGGGLPSRVDAVRLAVGKAGGRARGAVLASDAFFPFADGLEVAGAAGVTAA
                     VEPGGSVRDDEVIAAADRLGLALVFTGARHFRH"
     gene            complement(851..>2195)
                     /gene="dnaA"
                     /locus_tag="PK829_13675"
     CDS             complement(851..>2195)
                     /gene="dnaA"
                     /locus_tag="PK829_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOU42311.1"
                     /translation="RATFTTWLQGTRALSWADGEFVIGVRSDFAKDWLENRLYDLIAR
                     VLAGVTGRPTRVRFAVWSDDIIAPKTALANGAGRAEVVPVSANGGYKNGATIQANGAD
                     EENNQLNQRYSFPAFVVGPNNRLAHAAALSVAESPGKTYNPLFIYGGVGLGKTHLLHA
                     IGQRCRQDGHRVRYISAETFTNDLVMAIRTKQTPEFRERYRTVDVLLIDDIQFIAGKE
                     GTQDEFFHTFNDLHSHGKQVVISSDRPPKALATLEERLLSRFEWGLTADIQPPEEETR
                     RAILLAKADELGCPVPAAVIEFIAHQVRQNIRELEGALNKVIAYAQLTGAPIDQTLVD
                     RALADTLRRAERVTIDQVLDAIVRYYSVTHEALSSSSRKSTIAYPRQVAMYLARTETE
                     ASLPQIGLALGNRDHTTIMHGCEKISGLIDLDPQLRRDVLEIKAMLYAQAPVITH"
BASE COUNT          307 a          708 c          809 g          371 t
ORIGIN      
        1 ggtcatcgtc aaacacttgt ccccctgcgg gctggcccag gccgacagcg tcgccgcggc
       61 gctgcccgcg gccatcgccg ccgacccggt gtctgccttc ggtggggtca tcgccgccaa
      121 ccagccggta ggcgcggact tcgttgccgc gctggcggcg gccgagctgt tcgtcgaggc
      181 cgtcatcgcc ccggccttcg acgaggaagc cctggcctgg ttcgccgccc acaagaagaa
      241 ctgtcgtctg gtggcccgcg gaggggcgct ggagagagcc ggcggcgcgg cgctggagtg
      301 gcgcgccatc cgcggcgggc tgctggccca aacgggcgac aggggcgacc cgcccaacgt
      361 cgtctggcgc gttgtcagcc ggcgcgcgcc cacggatgag gaaatggcgg ccctgcgctt
      421 cgcctggctg gcctgccagc acgtcaagtc gaacgccatc gtcctggcgc gggccgaggg
      481 cgcggcggcg gcggtcgtgg gcatcggtgg ggggctgccc agccgggtag acgcggtgcg
      541 gctggccgtg ggcaaggcgg gcgggcgcgc gcgcggcgcg gtgttggcct ccgacgcctt
      601 cttcccgttt gccgacgggc tagaagtggc cggcgcggcc ggggtgacgg ccgctgtgga
      661 gccgggcggc tccgtgcgcg acgatgaagt gattgccgcg gccgaccggc tgggcctggc
      721 gctggtattc accggcgcac gccacttccg ccactgatcc ccaaggcaag gggcgcatcc
      781 cctgaaggat gcgcccccac cagcgccggc ggccactggc ccccggccgg ctcacctttt
      841 cacacagcgt ctagtgggtg atcaccgggg cctgcgcata gagcatggcc ttgatctcca
      901 acacgtcgcg gcgcagttgc gggtcgaggt cgatcaaccc ggagattttc tcgcaaccgt
      961 gcatgatggt cgtgtggtcg cggttgccca gggccaggcc gatctggggc agggaggctt
     1021 cggtctccgt gcgcgccagg tacatggcca cctgccgcgg gtaggcgatg gtgctcttac
     1081 ggctgctgct ggacagcgcc tcatgcgtga cgctgtagta acgcacgata gcgtcaagga
     1141 cctggtcgat ggtgacccgc tcggcgcggc gcagggtgtc agccagggcg cgatcgacca
     1201 gcgtctggtc gatgggcgcg ccggtcagtt gggcataagc gatgaccttg ttgagcgccc
     1261 cttccagctc gcggatattc tggcgcacct gatgggcgat gaattcgatg acggccgcgg
     1321 gcacagggca gcccagttcg tcggccttgg ccagcaagat ggcgcggcgc gtctcttctt
     1381 ccggcggctg gatgtcggcc gtcagccccc actcgaagcg cgacagcaga cgctcctcca
     1441 gcgtggccaa cgctttcggc ggccggtcgc tggagatgac cacctgcttg ccgtggctgt
     1501 gcagatcgtt gaaggtgtgg aagaactcgt cctgggtgcc ttccttgccg gcgatgaact
     1561 ggatgtcgtc gatgagcagg acatcgacgg tgcgatagcg ttcgcgaaac tctggcgtct
     1621 gcttggtgcg gatggccata accaggtcgt tggtgaaggt ctcggccgat atgtagcgca
     1681 cgcggtggcc atcctgccgg cagcgctggc cgatggcgtg gagcaggtgg gtcttgccga
     1741 ggccgacgcc gccgtagata aacaacgggt tataggtctt gccggggctt tcggccacag
     1801 acagagctgc ggcgtgggcc agccggttgt tggggccgac gacgaaggcc gggaaggaat
     1861 agcgctggtt gagctggttg ttctcttcat ccgcgccgtt ggcctggatg gtcgcgccgt
     1921 ttttgtaacc gccgttggcg ctcacgggca cgacctccgc gcggccggcg ccattggcca
     1981 gcgccgtctt gggagcgatg atgtcatcgg accagacggc gaagcgcacc cgcgtcggcc
     2041 ggccggtgac gccggccagg acgcgcgcga tgaggtcgta gagacggttc tccagccagt
     2101 ccttggcgaa gtcgctgcgc acgccgatga cgaactcgcc atcggcccag gacagcgccc
     2161 gagtgccttg cagccacgtc gtgaacgtcg cccgt
//
LOCUS       DAOSMQ010000523         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_50088_length_2185_cov_3.792958, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000523 DAOSMQ010000000
VERSION     DAOSMQ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50088_length_2185_cov_3.792958"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PK829_13680"
     CDS             <1..809
                     /locus_tag="PK829_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU42312.1"
                     /translation="PAGVASHPVLDQFLYSAILSLEATGYHLLTFVAGTRTDTAVYED
                     LYRRGQVSGFLVANTNDNDPRIACLIDRGIPFASFGRANDEWRFAWVDVDGGDGIAQV
                     VGHLVAGGHERIGLITWPAGSKAGSHRESGYLAGLRAAGIEPDPAWLFRGENSIQTGV
                     AGLSHWLTLPAEHRPTAVACVSDIIAVGVMNAAAAAGLHVGRDLAVTGYDDSSLAQFL
                     HPPLTSVRQPIAEVGQRLVALLLGQMGAEPATPAGVMLAPELVIRRSSQP"
     gene            854..1408
                     /gene="pgmB"
                     /locus_tag="PK829_13685"
                     /pseudo
     CDS             854..1408
                     /gene="pgmB"
                     /locus_tag="PK829_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017311381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
     gene            1463..>2185
                     /locus_tag="PK829_13690"
     CDS             1463..>2185
                     /locus_tag="PK829_13690"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOU42313.1"
                     /translation="MGLDELAAAVAFDPTRHVVESTFDPATQHHAETIFTLGNGTFAT
                     RGTFEEGYPGESALTMAHGLWDDTPIVFTELVNLPNWLRLNVTIDGDAFRMDQGRVLS
                     FRRYADLRAGVLRRDVLWQAPNGKTVDLHFERFIAYPRAHVAALRLLVTAVSHPCAVA
                     VRAGIDGHVANQDLLHLRLEDEGKAGGDAVWLRARTRHTGRYVAVAAALSAETSAATP
                     VEVAAELCPGFPTLLAAADLMPG"
BASE COUNT          295 a          849 c          715 g          326 t
ORIGIN      
        1 accccgccgg cgtggcctcc caccccgtgc tcgaccagtt tctctattcg gctattctca
       61 gcctggaggc gaccggttac cacctgctga ccttcgtcgc cggcacgcgc accgacacgg
      121 ctgtctacga agacctctac cgccgcggtc aggtcagtgg cttcctggtc gccaacacca
      181 acgacaacga cccgcgcatc gcctgtctga tcgaccgcgg catccccttc gccagcttcg
      241 gccgggccaa cgacgagtgg cgcttcgcct gggtcgatgt cgatggcggc gacggcatcg
      301 cccaggtcgt gggccatctc gtcgccggcg ggcacgaacg catcggcctg atcacctggc
      361 ccgccggctc caaggccggc tcccaccgcg agagcggcta cctggccggc ctgcgcgccg
      421 ccggcatcga acccgatccg gcgtggctct tccgcggcga gaactcaatc cagacaggcg
      481 tggccgggct gtcccactgg ctgacgctgc cggccgagca tcgccccacg gccgtagcct
      541 gtgtcagtga catcatcgcc gtcggcgtca tgaacgccgc cgctgctgcc gggctacacg
      601 tcggccgcga cctggccgtg accggctacg acgacagcag tctggcccag ttcttgcacc
      661 cgcccctgac ctccgtgcgc cagcccattg ccgaagtggg ccaacgcctg gtggcgctgc
      721 tgctgggcca aatgggcgct gagccggcca cgcccgcggg cgtcatgctg gccccggaac
      781 tcgtcatccg gagatcgagc cagccatgac caccgccgac cggcccgacg cgctggcttt
      841 gccgcgcgcc ctcatctttg acctcgacgg cgtcatcacc gacacggccg aataccacta
      901 cctggcctgg caacggctgg ccgacgagga ggggctgccc ttcggccgcg aagccaacga
      961 gcgctgccgc ggcgtcaccc ggcgcgagtc gctgctggtg gtgctgggcg gccggccgga
     1021 gagcgaagag cgcatgcagg cgatgatggc ccgcaagcag cgctattacg aacaggccct
     1081 cgaccgtatc ggcccggacg acctcttacc cggcgtggcc gccctgctgg acgccctcga
     1141 cgctgccgcc attccctacg ccatcgcctc ggccagcagg aacgcccggc cggtgctgga
     1201 gcggctgggc attgccgggc ggctggcggc cgtggccgac ggctacagcg tcgagcgcaa
     1261 caagccccac cctgacttgt tccgctatgc cgccgcggct ctggggctgc ccgcggccga
     1321 ctgcctggtg gtcgaggacg ccgccgcggg cgtcgtcgcc gggctggccg cgggcatgcc
     1381 cgtgctggcc ctcggcccgg ccgagcgctt cgccgggctg ctgcgccaca gccgcgtcac
     1441 ccgccgcgac gacctgcgcg acgtgggcct cgacgaactg gccgcggccg tcgccttcga
     1501 cccgacgcgc cacgtcgtcg aaagcacctt cgacccggcc acgcagcacc atgccgagac
     1561 catcttcact ctgggcaacg gcacgttcgc tacgcggggc acttttgagg agggctaccc
     1621 cggcgagtcg gccctgacca tggcccacgg cctgtgggac gacacgccca ttgtcttcac
     1681 cgagctggtc aatctgccca actggctgcg gctgaacgtc actatcgacg gcgacgcctt
     1741 ccgcatggac caggggcgcg tgctcagctt ccggcgctat gccgacttgc gcgccggcgt
     1801 cttgcgccgc gatgtgctct ggcaagcgcc caacggcaag acggtcgatc tccacttcga
     1861 gcgcttcatc gcctacccgc gcgcccacgt ggccgccctt cgcctgctgg ttacggccgt
     1921 ctcccacccc tgcgccgtcg ccgtccgcgc cggcatcgac ggccacgtgg ccaatcagga
     1981 cttgctccac ctgcggctgg aggacgaggg caaggccggc ggcgatgcgg tctggctgcg
     2041 cgcccgcacg cgccacaccg gccggtatgt ggccgtggcg gcagcgctgt cggccgagac
     2101 ttccgcggcc acgccggtcg aggtggccgc cgagctgtgc cccggcttcc cgacgctgct
     2161 ggccgcggcc gacctgatgc ccggc
//
LOCUS       DAOSMQ010000524         2179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_50286_length_2179_cov_4.723164, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000524 DAOSMQ010000000
VERSION     DAOSMQ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50286_length_2179_cov_4.723164"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PK829_13695"
     CDS             <1..789
                     /locus_tag="PK829_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOU42314.1"
                     /translation="HNERNPIFPKMLRPEPIPPNVDDGLSYVGRLGADVALITDGDAD
                     RVGLGDENGHFIDQLRVYGLLGYYFLEVRGERGPIVKTISTTKMLNKLGALYNVPIYE
                     TGVGFKYIAPKMKEVDAMLGGEESGGYAFRGHAPDRDGILAGLYLLDMMVQTGKRPSE
                     LLDWLFEKVGPHYYDRIDTAFETARRDEVQARVQAARPERIAGLKVTDISTLDGFQFH
                     MEDGGWLLIRFSGTEPIIRVYCETTHADKVQDILQDGLRIAGLR"
     gene            799..1293
                     /locus_tag="PK829_13700"
     CDS             799..1293
                     /locus_tag="PK829_13700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOU42315.1"
                     /translation="MSKAFQDYYPDEVSYCYGCGRLNEHGLQIKSYWDGEETVCHYQP
                     RPYHTAVPGYVYGGLLASLIDCHGTGTAAAAAYRDQGRPMDSDPPLRFLTASLHVDYL
                     KPTPIDRPLELRGRVTEIKGRKVIVAVELYAHDELCVRGEVVTVQVPDDFMARLMGEA
                     AGEG"
     gene            1295..2110
                     /locus_tag="PK829_13705"
     CDS             1295..2110
                     /locus_tag="PK829_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU42316.1"
                     /translation="MSDGRPALVDTHCHLDFVSLKEDIEAVVARAAEAGVTTIIVPAL
                     DLDNAPAVLALAGRFPGIYAAVGVHPNSAAGWRDEWIDDLRGLAGHEKVVAIGEIGLD
                     YYWDKTPPATQHHALAAQLDLAAELGLPVILHNRDASADVLAQLTAAAQRRPRPDNTP
                     PGVLHSFSAGAEMAAAALALGYALGFTGPLTFKKADDLRAIAAAVPLDRIVVETDAPY
                     LTPEPYRGRRNEPAYVRFVAERLADVRGLPYDAIAQATTANAHRLFPRLSPTY"
BASE COUNT          373 a          777 c          676 g          353 t
ORIGIN      
        1 cacaacgagc gcaaccccat cttccccaag atgctgcggc cggagcctat tccgcccaac
       61 gtcgatgacg ggctgagcta cgttggccgc ctgggggccg acgtggccct catcaccgac
      121 ggcgacgccg accgcgtcgg cctgggcgac gagaacggcc acttcatcga ccaactgcgc
      181 gtctacggcc tgctgggcta ctacttcctg gaagtgcgcg gcgagcgcgg ccccatcgtc
      241 aagaccatct cgacgacaaa gatgctcaat aagctcggcg cgctctacaa cgtgcctatc
      301 tatgagacgg gcgtgggctt caaatacatt gctccaaaga tgaaggaagt ggacgccatg
      361 ctcggcggcg aggagagcgg cggctatgct ttccgtggtc acgcgcccga ccgcgatggc
      421 atcctggccg gcctttacct gctggatatg atggtgcaga cgggcaagcg gccgtcggag
      481 ttgctcgact ggctgtttga gaaggtcggc ccgcactact acgaccgcat cgacaccgct
      541 ttcgagacgg cgcggcgtga cgaggtgcag gcccgtgtcc aggccgcccg cccggagcgt
      601 atcgccggcc tgaaggtgac cgacatcagc actctcgacg gcttccagtt ccacatggag
      661 gatggcggct ggctgctcat ccgcttcagc ggcaccgagc cgatcatccg cgtctactgc
      721 gagacaaccc acgccgacaa ggttcaggat atcctccaag acggcctacg catcgccggc
      781 ctgcgctaac cagctaccat gtcaaaagcc ttccaggact attaccccga cgaagtaagc
      841 tactgttacg gctgcggccg gctcaacgag catggcttgc agatcaagag ctattgggac
      901 ggcgaagaga cggtctgcca ctaccagccg cgcccctacc acaccgccgt gcctggctac
      961 gtctatggcg gactgctggc ctcgctcatt gactgccacg gtacgggcac ggccgccgcc
     1021 gcggcctacc gcgaccaggg ccggccgatg gacagcgacc cacccctgcg tttcctgacg
     1081 gcctcgctgc acgtcgacta cctcaagccg acgcccatcg accggccgct ggagttgcgc
     1141 ggccgggtca cggagatcaa ggggcgcaag gtcattgtcg ccgtggagtt gtatgcccat
     1201 gacgaactgt gcgtccgcgg cgaagtcgtc accgtgcagg ttcccgacga cttcatggcc
     1261 cggctgatgg gcgaggccgc gggggaggga taacgtgagc gacggccggc cggcgctggt
     1321 tgacactcac tgccatctcg acttcgtttc cttgaaagag gatattgaag ccgtcgtggc
     1381 ccgggcggcc gaagccgggg tgacgacgat catcgtgcct gcgctcgacc tggacaacgc
     1441 gccggccgtg ctggccctgg ctgggcgctt ccccggcatc tatgccgcgg tcggcgtcca
     1501 ccccaactcg gccgccggct ggcgcgatga gtggatcgac gacttgcgcg gcctggccgg
     1561 gcacgagaag gtcgtcgcca tcggcgagat tggcctggac tattactggg acaagacgcc
     1621 gccggccacg cagcaccacg ccctggcggc gcaactcgac ctggccgccg agctgggcct
     1681 gccggtcatc ctccacaacc gcgacgccag cgccgatgtg ctggcccagc taacggcggc
     1741 ggcgcagcgg cgaccgcggc ccgacaacac cccgcccggc gtgctgcact ccttctccgc
     1801 cggcgcggaa atggccgcgg ccgcgctggc gctgggctac gctcttggct tcaccgggcc
     1861 attgaccttc aagaaagccg acgacctgcg ggccatcgcc gccgccgtgc cgcttgaccg
     1921 cattgtggtg gagaccgacg cgccctacct gacgcccgag ccttaccgcg gccgccgcaa
     1981 cgaacccgcc tacgtccgct tcgtggccga gcgcctggcc gacgtgcgtg gcctgcccta
     2041 tgacgccatc gcccaggcca ccaccgccaa tgcccatcgt ctcttcccgc gtctgtcacc
     2101 aacctactga cctaccgatc attgcccacc atgctctcca tcctcatcgt caactggaac
     2161 gtccgtgacc tgctgcgcg
//
LOCUS       DAOSMQ010000525         2178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_50331_length_2178_cov_4.073952, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000525 DAOSMQ010000000
VERSION     DAOSMQ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50331_length_2178_cov_4.073952"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK829_13710"
     CDS             complement(<1..207)
                     /locus_tag="PK829_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU42317.1"
                     /translation="MANSQPRPQETWDDGAAYEQYVGRWSRRVAAPFVQQLAVPAGAR
                     WLDVGSGSGALSEAVLAAAGPAELL"
     gene            complement(268..1131)
                     /locus_tag="PK829_13715"
     CDS             complement(268..1131)
                     /locus_tag="PK829_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOU42318.1"
                     /translation="MSPYGSQSMIAPLQSVLVRRPDEAFGAADPARWHYTATPDLLLA
                     RQEHDAFVNTLLAHGVEVAYHDAPLPDHADALFVHDPALITDRGAILLRMGKPQRAGE
                     EEAMAVTLAQLGVPIHYRLHGAATAEGGDLLWLDERTLCVGLGFRTNAAGFDQLAEAL
                     AGSGVALVPVQLPYFEGPEACLHLMSLISLVDVDLAVVYRPLLPVPFYQLLESRGVRL
                     IDVADEEFATMGTNVLALAPRECILLDHNIHTARALEAAGCRVYPYRGDEMSLKAEGG
                     PTCLTRPILRG"
     gene            complement(1192..1851)
                     /locus_tag="PK829_13720"
     CDS             complement(1192..1851)
                     /locus_tag="PK829_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42319.1"
                     /translation="MTGLLWQIFNVIKETLNRTIKFPELLLTLLGLSATKRLRLRVVI
                     LRDERGLPLASNEEAQPAYEEARRIFARMAGVSVEPAGWRVVTAPHAAPKAALDVRCT
                     DGAWQDDLGAAGGFFRSLMAKTTSGTLIGYGQPLTVFIVRSMSTHNGCSLGAATDYVT
                     VEAKTLRATRRLLAHEVAHACGLWHSKNPANLMAPRGPGEELAHWQAAILRTSRHVTF
                     F"
     gene            2054..>2178
                     /locus_tag="PK829_13725"
     CDS             2054..>2178
                     /locus_tag="PK829_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOU42320.1"
                     /translation="MVAEQIAGRGIDDDAVLQAMRLVPREHFVPDAERAYAYDDTP"
BASE COUNT          353 a          701 c          801 g          323 t
ORIGIN      
        1 cagcagctca gccggaccgg cggcggccaa gaccgcctcg ctcagcgctc cgctgccgga
       61 gccgacatcc agccagcgcg ccccggcggg cacggccaac tgctgcacga acggcgcggc
      121 cacgcggcgg ctccagcggc cgacgtactg ctcgtaggcc gcgccgtcgt cccatgtttc
      181 ttgcggtcgt ggctgcgagt tggccattat cgtatctttt caactcgccg ggcgagcggc
      241 tgggcgggtg gcgcggggaa ggctggatca gccccgcagg atcggccgcg tcaggcaggt
      301 tggcccgccc tcggccttca acgacatctc gtcgccgcgg taggggtaga cgcggcagcc
      361 ggccgcctcc agcgcgcgcg ccgtgtggat gttgtggtcg agcaggatgc actcgcgcgg
      421 ggccagggcc agcacgttcg tgcccattgt ggcgaactcc tcatcggcca cgtcgatgag
      481 ccggacgccg cgcgattcca gcagttgata gaagggcacg ggcagcaacg gccggtagac
      541 cacggccaga tcgacatcga ccaggctgat gagcgacatc aggtgcaggc acgcctctgg
      601 cccctcgaaa tagggcagct gcacggggac gagggccacg cccgacccgg ccagcgcctc
      661 ggccagctgg tcgaagccgg cggcgttggt gcggaagccc aggccaacgc acagcgtccg
      721 ctcgtcgagc cacagcaggt cgccgccctc ggccgtggcc gcgccgtgga gccggtaatg
      781 gataggcaca cccaactgcg ccagcgtgac ggccatggcc tcctcctcgc cggcgcgctg
      841 cggcttgccc atgcgcagga ggatggcccc gcggtcggtg atcagcgccg ggtcgtggac
      901 gaaaagggcg tcggcgtggt cgggcagcgg cgcgtcgtgg taggccacct ccacgccgtg
      961 ggcgagcagg gtgttgacga aggcatcgtg ctcctggcgg gccagcagca aatcgggagt
     1021 cgcggtgtag tgccagcggg cggggtcggc cgcgccaaag gcctcgtctg gccggcggac
     1081 cagcaccgac tgaagagggg cgatcatgga ttgggagccg tagggggaca tggtgaggga
     1141 tgaaggataa gggatgagga agcagtaggt cagtcggcgg cggtcggcgg tctaaaagaa
     1201 ggtgacatgg cgcgacgtgc gcagaatggc ggcttgccag tgggccagtt cctcgccggg
     1261 gccgcgcggg gccatgaggt tggccgggtt cttggaatgc cacaggccgc aggcgtgggc
     1321 aacctcgtgg gccagcaggc ggcgggtggc gcgcagtgtt ttggcctcga cggtcacgta
     1381 gtcggtcgcc gcgcccagcg aacagccgtt gtgggtggac atgctgcgga cgatgaagac
     1441 ggtcaggggt tggccgtagc cgatgagcgt gccgctggtc gtcttggcca tcaggctgcg
     1501 gaagaagccg ccggccgcgc ccaggtcatc ctgccaggcg ccgtcggtgc agcgcacgtc
     1561 gagggccgct ttcggcgcgg catggggcgc ggtgacgacg cgccagccgg ccggctcgac
     1621 cgacacgccg gccatgcggg cgaagatgcg gcgggcctcc tcataggccg gctgggcctc
     1681 ctcgttgctg gccaggggca ggccgcgctc gtcgcgtaga atgaccacgc gcaggcgcag
     1741 cctcttggtg gcgctaaggc ccagcagggt cagcagcaac tccgggaatt tgatcgtccg
     1801 gttcagcgtt tccttgatga cgttaaagat ctgccacagt aatccggtca tgagggcctt
     1861 ccagtaattc cgctgcccag agattagccc aaagtcgcca tgacgcaaaa ccggccgccc
     1921 cgtggcgcga ttctcccgaa tcgcggcgca ggcgattcgg gagaatcgcg ccacgttcca
     1981 ttgcacacca acaactttgc gttagattcc ctctatggaa gaggatgcgc gctttgccgc
     2041 ggcgcgggag cggatggtgg ccgagcagat tgccggacga ggcatcgacg acgacgccgt
     2101 gctgcaggcc atgcgtctcg tcccgcgcga gcacttcgtg ccagacgccg agcgcgccta
     2161 tgcctacgac gacacccc
//
LOCUS       DAOSMQ010000526         2177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_50363_length_2177_cov_3.991989, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000526 DAOSMQ010000000
VERSION     DAOSMQ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50363_length_2177_cov_3.991989"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PK829_13730"
     CDS             <1..1094
                     /locus_tag="PK829_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU42321.1"
                     /translation="ADFFDRDPYPGIRRWAELLPPLAPGLSMGEGGTPLVAAPRLAEW
                     AGFDGELYVKDESRNPTGSHKDRLNLCTVSAAVLSGAPGITVASSGNHGAAAAAYAAR
                     AGLPCVLFITEGTEPHLVRMVLAYGAAVVPLPRPLRRVLMAELVHRAGFHPVSSITAT
                     HTGHPFGPEGYKTIAYELYRQLGDRMPAAVFVPTAYAELLYGVWMGFRELAQVGRRGP
                     APQLFACEPAAGAPLRVALAQGRPVVQIEEAPTAAYSIVVPANGYRGVLAVRESEGDA
                     LAVTDEEMLMAQTALGRAGLWAELSGAAALAGLRQAARAMTFDGPVVCLMTSTGLKDQ
                     GLPAPVLPPVAPTWAAVRQTLHDVYGLSV"
     gene            1110..>2177
                     /locus_tag="PK829_13735"
     CDS             1110..>2177
                     /locus_tag="PK829_13735"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU42322.1"
                     /translation="MPAETVTPSRQQYLDVKAQYPDAIVFFRLGDFYETFDDDAAVVA
                     RELDLILTSRTQGKSGRIPMAGVPHHSVENYIARLIAKGYKVALCEQMSTDLINGVMP
                     REVTRVFTAGTVVEPGMLEAGRNNYLAAAVFDDGRAGLAYADITTGEFATAHLSGRNE
                     LMEELARLAPVELLLPEGAEFTHPAIKTISPLPAWRFELGHARQTLLRHFGVATLDAF
                     GCEGKPLATRAAGAVLHYLQETQRGRVGQMQRLTTYSTAGYMALSEATRRNLELTESL
                     SGAREGTLLYVLNKTVTPMGARLLRQRLTRPLLSVGPLNARLDQVETLVADGLLRAEL
                     RGALKGLPDLERLTNRLLGGRA"
BASE COUNT          315 a          761 c          745 g          356 t
ORIGIN      
        1 cagccgattt cttcgaccgc gatccctacc ccggcattcg ccgctgggca gaactgctgc
       61 cgcctctagc gccggggctg tcgatgggcg agggcggcac gcccctggtg gccgcgccgc
      121 gcctggccga gtgggccggc ttcgacggtg agctgtatgt caaggatgag agccgcaacc
      181 ccaccggtag ccacaaagac cgcctgaacc tttgcacggt cagtgccgcc gtgctcagcg
      241 gcgcgccggg catcaccgtg gcctcgtcgg gcaaccacgg cgcggccgcc gccgcctacg
      301 ccgcgcgggc cgggttgcct tgtgtcttgt tcatcaccga gggcaccgag ccgcacctgg
      361 tacgcatggt tttggcctat ggcgcggcgg tcgtgccgct gccgcggccg ttgcggcgcg
      421 tgctgatggc cgaactcgtt caccgcgccg gcttccaccc tgtcagcagc atcaccgcca
      481 cccacactgg ccaccccttc ggcccagagg gctacaagac cattgcttac gagctgtacc
      541 ggcaattggg cgaccggatg ccggcggccg tctttgtgcc cacggcctac gccgagttgc
      601 tctatggcgt ctggatgggc ttccgggaac tggcccaggt gggccggcgc ggccccgcgc
      661 cacagttgtt tgcctgtgag ccggccgcgg gcgcgccctt gcgtgtcgcc ctggcccagg
      721 ggcgaccggt cgtgcagatt gaggaagcgc ccacggcggc ctatagcatt gtcgtccctg
      781 ccaatggcta ccgcggcgtc ctggccgtgc gcgagagcga gggcgacgct ctggccgtca
      841 ccgatgagga gatgctgatg gcgcagacag cgctgggtcg cgcgggcctg tgggcggagc
      901 tatccggcgc ggcggcgctg gccgggctgc gccaggccgc gcgggcgatg actttcgatg
      961 ggcctgttgt ctgcctgatg acctctaccg gtctgaagga ccaggggttg cccgcgccgg
     1021 tgctgccgcc ggttgcgcca acctgggcgg ccgtgcggca aacgcttcac gatgtctacg
     1081 ggctgtccgt ctagcgccat cgccacgcca tgccggccga aaccgtcacc cccagccgcc
     1141 agcagtacct cgacgtcaag gcgcaatacc ccgacgccat cgtcttcttc cgcctgggcg
     1201 acttctacga gacattcgac gacgatgccg ccgtcgtggc ccgcgaactc gacttgatcc
     1261 tgaccagccg gacgcagggc aagagcggtc gcatccccat ggccggtgtg ccccaccact
     1321 cggtcgagaa ctacatcgcc cggctgatcg ccaagggcta taaggtcgcc ctgtgcgagc
     1381 agatgagcac cgacctgatc aatggcgtca tgccgcgcga ggtgacccgc gtcttcaccg
     1441 ccggaacggt cgttgaaccc gggatgctag aggctggccg caacaactac ctggccgcgg
     1501 ccgtgttcga cgacggccgc gcgggcctgg cctatgccga catcaccacg ggtgagttcg
     1561 ccacggcgca tctgtccggg cggaacgaac tgatggagga actggcccgt ctggctccgg
     1621 tcgagttgct cctgccggag ggtgcggagt tcacccaccc ggccattaag accatcagcc
     1681 cgttgccggc ctggcggttc gaactgggcc atgcccgcca gacgttgctg cgccatttcg
     1741 gcgtggccac gctcgacgcc tttggctgcg aaggcaagcc gttggcgacg cgcgccgccg
     1801 gggctgtgct ccactacttg caggagaccc agcgcggccg cgtgggccag atgcagcgcc
     1861 tgaccaccta ttcgacagcc gggtacatgg ctctatcgga ggctacgcgg cgcaacctgg
     1921 agctaaccga gtcgctcagc ggcgcgcgcg agggcacgct gctgtacgtc ctcaacaaga
     1981 ccgtcacgcc gatgggcgcg cgcctgctgc gccaacgtct gacgcggccg ttgcttagcg
     2041 tcggcccgct gaacgcgcgc cttgatcagg tggaaacgtt ggtggccgat ggcctgttgc
     2101 gggccgagtt gcgcggggcg ctaaaggggc tgcccgacct ggagcggctg accaaccgcc
     2161 tgctgggcgg gcgggcc
//
LOCUS       DAOSMQ010000527         2161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_50899_length_2161_cov_3.490028, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000527 DAOSMQ010000000
VERSION     DAOSMQ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50899_length_2161_cov_3.490028"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..633)
                     /locus_tag="PK829_13740"
     CDS             complement(55..633)
                     /locus_tag="PK829_13740"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HOU42323.1"
                     /translation="MANPARGAVRRVIATRPAAWLLARTIHYIDRAVLRLSGGRTTAA
                     ALFSGLPVLAVTTTGAKSGQPRTVPLVAVPDGDRLILIASNWGQSRHPGWYYNMKANP
                     AVRVVGGDGPPGRLYMAREVAGEERAACWAKAVAVYPGYEAYARRAGREIGVVVLTLL
                     EDLSDLAALAAAVEEPERDYEEYLAKRNHGKS"
     gene            complement(767..>2161)
                     /locus_tag="PK829_13745"
     CDS             complement(767..>2161)
                     /locus_tag="PK829_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU42324.1"
                     /translation="ADGTFRRGRLAPMKPIADEAAAQTPSLRHVIVLRRAGNDVATSD
                     VAMTPGRDHWWHELVAAQPDTATTADTAAEDTLLILYTSGTTGRPKGIVHTHCGFPVK
                     AAQDMAFGTDVHGAADGGDDRIYWITDMGWMMGPWLVYGATLLGATFCFYDGSPDYPD
                     VDRLWALAERHRLTHLGVSPTLIRALAPHGAEPARRHDLSSVRFFASTGEPWNPDPWR
                     WLFDVVGEGRRPIINYSGGTEISGGIVMGNPILPLKPTAFAAPCPGIAADVVDSAGRS
                     VRGQVGELVIRAPWIGMARGFWHDRQRYLDTYWSQLPGVWVHGDWAAVDEDGMWYILG
                     RSDDTIKIAGKRLGPAEVESVLVAHPAVREAAAVGVPDAVKGSALVCFVVLSAGHAPS
                     EALAAELRALVGAELGKPLTPQAVRFVSDLPKTRNAKLMRRVIRSAYLGQDPGDTSSL
                     VNPEVIAELRRDEE"
BASE COUNT          309 a          775 c          733 g          344 t
ORIGIN      
        1 ccgggtagcc gatcccggcc ggcctcagcc gttcgcttca agcgcaaccg atcattacga
       61 cttgccgtgg ttgcgtttcg ccaggtattc ctcgtaatcg cgctccggct cctcgaccgc
      121 tgccgccagg gcggcgagat cactcaggtc ttccagcagc gttaatacca ccaccccaat
      181 ctccctcccc gcccgccgcg catacgcctc ataccccgga tagaccgcca ccgccttcgc
      241 ccaacacgcc gcccgctcct cccccgccac ctcgcgggcc atgtagagac ggcccggtgg
      301 gccgtctcca cccacgacgc gcaccgccgg gttggctttc atattgtaat accaccccgg
      361 gtgacggctc tgcccccagt tcgaggcgat cagaatgagg cggtcgccgt cgggtacggc
      421 caccagcggc acggtgcgcg gctgcccact cttcgccccg gtcgtcgtca cggccaggac
      481 gggcaggccg ctaaagagcg ccgccgccgt cgtccggccg ccgctcaggc gcagcacggc
      541 ccggtcgatg tagtgaatcg tccgcgccag cagccacgcc gccggccgcg tggcgatcac
      601 ccgccgcaca gcccctcgcg ctggattagc catcggcctc cttctggggg gcgctcacgc
      661 aaaggcgcaa agaagcaaag gcgcaaagga agaaagctta ttcttctttg cttcttgtct
      721 tcttgtcttc tttgcgtctt tgctcccttt gcgtcctttg cgtgagctat tcttcatcgc
      781 gtctcagttc ggcgatcact tccgggttga ccaggctcga cgtatccccg gggtcctggc
      841 ccaggtaggc gctgcggatg acgcgccgca tcagcttggc attgcgcgtc ttgggcaggt
      901 cgctgacaaa gcggacggcc tgcggcgtca gcggcttgcc caactctgcg cccaccagcg
      961 cgcgcaactc ggccgccagc gcctccgacg gcgcgtgccc ggcgctcagg acgacgaagc
     1021 agaccaacgc gctgcctttg accgcgtcgg gcacacccac ggcggcggcc tcgcgcacgg
     1081 ccgggtgggc caccagcacc gactcgacct cggccggccc cagccgcttg ccggcgatct
     1141 tgatcgtgtc gtcggagcgg ccgaggatgt accacatgcc gtcctcatcg accgcggccc
     1201 agtcgccatg cacccagacg ccgggcagtt gcgaccagta ggtgtccaga tagcgctgcc
     1261 ggtcgtgcca gaagccgcgg gccatgccga tccacggcgc gcgtatgacc agttcgccca
     1321 cctggccgcg cacggagcgg ccggcgctat ccaccacgtc ggcggcgatg ccggggcagg
     1381 gcgcggcgaa ggcggtcggc ttgagcggca ggatggggtt gcccatgacg atgccgccgc
     1441 tgatctccgt gccgccggag tagttgatga tcggccgccg cccctcgccc accacgtcga
     1501 acagccagcg ccacgggtcg gggttccacg gctcgcccgt gctggcgaag aagcgcaccg
     1561 acgacaggtc gtggcggcgc gccggctccg cgccgtgggg ggccagggcg cggatgagtg
     1621 tcggcgagac gcccaggtgg gtcagccggt ggcgttcggc cagcgcccag aggcgatcga
     1681 cgtcgggata gtcgggcgag ccgtcgtaga agcagaacgt cgcccccagc agcgtcgccc
     1741 catagaccag ccacggcccc atcatccagc ccatgtcggt gatccagtag atgcggtcgt
     1801 cgccgccgtc ggccgcgccg tggacgtcgg tgccgaaggc catgtcctgg gcggccttga
     1861 ctgggaagcc gcagtgggtg tggacgatgc ccttgggccg gccggtcgtg ccgctggtgt
     1921 agaggatgag cagggtatcc tcggcggcgg tgtcggccgt ggtcgccgtg tccggctggg
     1981 cggcgacgag ttcgtgccac cagtggtcgc ggccgggggt catggccaca tcagatgtgg
     2041 ccacgtcgtt gccggcgcgg cgcaggacga tgacgtggcg cagcgagggc gtttgcgcgg
     2101 cggcctcgtc ggcgatgggc ttcatcggcg cgagacggcc gcggcggaat gtgccgtcgg
     2161 c
//
LOCUS       DAOSMQ010000528         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_51090_length_2155_cov_5.338095, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000528 DAOSMQ010000000
VERSION     DAOSMQ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51090_length_2155_cov_5.338095"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="PK829_13750"
     CDS             complement(<1..1739)
                     /locus_tag="PK829_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU42325.1"
                     /translation="MIICPNCQTENRPGTKFCRTCGKPLPADPKATQLSTPLPAAPAS
                     GFPWPAPSSGVGAPAQPGQCPNCGSPVQPNTMFCPICGHALRAGGTAAGAAGMGTLVM
                     PKAERPALVINWPGGQKDERPLEKPVMRLGRAPDNDIVINFPTVSGHHLRLETDGTGD
                     GVRVTDLGSTNGTLHNGLQMPANAPRTVYAGDVLRIGDLTGNSLSLSLKTTGAGLQTR
                     PLGMQQLAQLQQAVIGRDPSCEVHLDHPSVSRRHAEIRRQPDGSFVIRDLGSANGTFV
                     DGQRVVAWLPLAKGAVVQIGPYKMVYDLEAKALTTSMSRGHRLDTVNLGKQVAGGRMI
                     LTDVSLAVLAGEFISLVGGSGAGKSTLLRAMNGFNPATQGQMLIDGEQLYPNLDAYRM
                     IMGYVPQDDIIHRELTVGKALYYAAKLRLPDAGKAEIDKRIHEVLEMVDMGAHAHKPV
                     RVLSGGQRKRVSIAVELLAQPDLLFLDEPTSGLDPGLEKKMMYDLNRLADRGKTIVLV
                     THATANIEQCDHVAFLSQGYLSYYGPPGEAIQYFHAQDFADIYLKLTQEVNPAEGKPA
                     PDNLQAVFPAVQAR"
     gene            complement(1783..>2155)
                     /locus_tag="PK829_13755"
     CDS             complement(1783..>2155)
                     /locus_tag="PK829_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42326.1"
                     /translation="GLSTAYAPFAGGQMLWRGDNAQIYVLTNDGHWAQYDDLWQEGEA
                     EFTCGEANTPPAPVRGFGRVWCDHAAVRESLGAATAAEIGDSGGRAQDFVNGLLLAGP
                     DGSTFVLIGETATWRRVWPNE"
BASE COUNT          308 a          694 c          772 g          381 t
ORIGIN      
        1 cgcgcctgca cggccgggaa gaccgcttgc aggttgtcgg gcgcgggctt gccctcggcc
       61 gggttcactt cctgggtcag cttcaggtag atgtcggcga agtcctgggc gtggaagtac
      121 tggatggcct cgccgggcgg gccgtagtag gacaggtagc cctggctcag gaaggccacg
      181 tggtcgcatt gctcgatgtt ggccgtggcg tgggtgacaa ggacgatggt cttgccgcgg
      241 tcggccagcc ggttgaggtc gtacatcatc ttcttctcca gcccggggtc caggccggag
      301 gtcggctcgt ccagaaagag caggtcgggc tgggccagca gctccacagc gatgctgacg
      361 cgcttgcgct ggccgccgct gaggacgcgc actggcttgt gggcgtgcgc gcccatgtcc
      421 accatctcca gcacttcgtg gatgcgcttg tcgatttcgg ccttgccggc gtccggcagg
      481 cgcagcttgg cggcatagta aagcgccttg ccaacggtca gttcgcggtg gatgatgtcg
      541 tcctggggca cgtagcccat gatcatgcgg taggcgtcga ggttggggta gagctgctcg
      601 ccgtcgatga gcatctgccc ctgcgtggcc gggttgaagc cgttcatggc ccgcagcagc
      661 gtgctcttgc ccgcgccgct gccgccgacg agcgagatga actcgcccgc cagcacagcc
      721 agcgatacgt cggtcaggat catccgcccg ccggcaacct gcttgcccag attaacggta
      781 tcgaggcggt ggccgcggct cattgaggtc gtcagggcct tggcctccag gtcatagacc
      841 atcttgtatg gcccgatctg cacaaccgcg cccttggcca gcggcagcca ggcgacaacg
      901 cgctgcccgt ccacgaacgt gccgttggcg ctgcccaggt cgcgaatgac gaagctgccg
      961 tcaggctggc ggcggatttc ggcgtggcgg cgagagacgg aggggtggtc gaggtgcact
     1021 tcgcaagacg ggtcgcggcc gatgaccgcc tgctgcaatt gggccagctg ctgcatcccc
     1081 agtgggcgcg tctgcaagcc agccccggta gtcttcagcg acaggctcag cgagttgccg
     1141 gtcaggtcgc caatgcgcaa cacatcgccg gcgtagaccg tccgcggcgc gttggccggc
     1201 atctgtagac cgttgtggag tgtgccgttg gtgctgccca ggtcagtaac ccgcaccccg
     1261 tcccccgtgc cgtcggtttc caggcgcaag tgatggccgg agacggtcgg gaagttgatg
     1321 acgatatcgt tgtcgggcgc gcggcccagg cgcatgaccg gcttttccag cggacgctca
     1381 tccttctggc cgccgggcca attgatgacc agcgccggcc gttccgcttt gggcatcacc
     1441 agcgtgccca tgccggccgc gccggcggcg gttccgcccg cccgcaaggc gtggccgcaa
     1501 atggggcaga acatcgtgtt gggttgcacg ggcgagccgc aattagggca ctggccaggc
     1561 tgtgccggcg cgcccacacc gctggagggg gccggccagg ggaagccgct ggccggggcc
     1621 gcgggcaggg gcgtcgagag ctgggtggct ttggggtcgg ccggcagagg cttgccgcag
     1681 gtgcggcaga acttcgtgcc ggggcggttc tcagtttggc aattggggca gatgatcatc
     1741 gcaacttcct tgctagaagg gttccccctc gtttgggccg gctcactcgt tgggccatac
     1801 gcgccgccag gtggccgtct cgccgatcag cacaaaggtc gatccgtctg ggccggccag
     1861 tagcaagcca ttgacaaagt cctgcgcccg gccgccgctg tcgccgatct ccgccgccgt
     1921 ggccgcgccc agactctcgc gcaccgcggc gtggtcgcac cagacgcggc cgaagccgcg
     1981 caccggcgct ggcggggtgt tggcctcgcc acaggtgaat tcggcctcgc cctcctgcca
     2041 gaggtcgtcg tattgcgccc agtggccgtc gttggtgagg acgtagattt gcgcgttgtc
     2101 gccccgccag agcatctgcc cgccggcaaa aggcgcgtag gcggtgctga ggccc
//
LOCUS       DAOSMQ010000529         2147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_51347_length_2147_cov_4.340822, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000529 DAOSMQ010000000
VERSION     DAOSMQ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51347_length_2147_cov_4.340822"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PK829_13760"
     CDS             <1..643
                     /locus_tag="PK829_13760"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU42327.1"
                     /translation="ALVIDCSTISPEATRAIATRLSARGVHMLDAPVSGGSEGAAKGT
                     LSIMVGGDAAQVARAMPILGAMGKTITHVGGQGAGQLAKLVNQVLVVVTMQAVGEALL
                     LAQAGGLDLERTLAAVGGGAAGSWMLNNRGPQVIRRDWRPGFTIDLQQKDLRLVLETA
                     DTLGIPLPSTAQVFQYYRALQQRGLGGEGNHALVKALEQLAGQEIGGATATPE"
     gene            787..1065
                     /locus_tag="PK829_13765"
     CDS             787..1065
                     /locus_tag="PK829_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42328.1"
                     /translation="MASKREFTLEEAQRIGDQIGVDWSQYDLEQFRMGLAVELEHGVH
                     DLQTNVTGDDELVTGKIALAHLKEIRDYYSRLAVMEREAEAYWATQDK"
     gene            complement(1157..1810)
                     /gene="folE"
                     /locus_tag="PK829_13770"
     CDS             complement(1157..1810)
                     /gene="folE"
                     /locus_tag="PK829_13770"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOU42329.1"
                     /translation="MIDARHLDNTAPDPGGELTDGPVLAASCKEKVADSVRTILRAVG
                     EDPQREGLRDTPNRVARAYDELLLGYRVDPVELLNNALFEASYSEMVVVSDIDFYSLC
                     EHHMLPFFGRAYVAYIPNRKVIGLSKIPRIVEVYARRLQIQERMTRQIAELIDELVQP
                     LGVAVVVDGLHMCMAMRGVKKSNARMRTSALLGTFADNPKTRAEFMEHIGPVARSLV"
     gene            1936..>2147
                     /locus_tag="PK829_13775"
     CDS             1936..>2147
                     /locus_tag="PK829_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42330.1"
                     /translation="MNRDQQDQAADARFDARRAARQAHADKSAKGIVDFDEMVKLDAR
                     REMIHPSDGLGLERILGTSDLLEINFL"
BASE COUNT          391 a          711 c          706 g          339 t
ORIGIN      
        1 cgcgctggtc atcgattgca gcaccatcag cccggaggcc acccgcgcca tcgccacccg
       61 gctgagcgcg cgcggcgtcc acatgctcga cgcccctgtc agcggcggca gcgagggcgc
      121 ggccaagggc acgctcagca tcatggtcgg cggcgacgcc gcccaggtcg cgcgggccat
      181 gcccatcctg ggggcgatgg gcaagacgat cacccacgtc ggcggccagg gcgcggggca
      241 actggccaag ctcgtcaacc aggtgcttgt cgtcgtcacc atgcaggccg tgggcgaggc
      301 gctgctgctg gcccaggcgg gcgggctgga tttggagcgg acgctggcgg ccgtcggcgg
      361 cggcgcggcc ggcagctgga tgctcaacaa ccgcggcccg caggtcattc gccgcgactg
      421 gcggccgggc ttcaccatcg acttgcagca aaaggacttg cgcctggtgc tggagacggc
      481 cgacacgctg ggcatccccc tgccgtccac ggcgcaagtc ttccaatact atcgcgccct
      541 gcaacagcgc ggcctgggcg gcgagggcaa ccacgccctg gtgaaggcgt tggagcagct
      601 ggccgggcag gagatcggcg gcgcgacggc cacgccggaa tagaggccgc ccaatccacc
      661 cttcccgccc ggcgatggcc ttcgcccacc tccgcgcgcg gctggccaca caagccactc
      721 ttgctgaaat gaggtatagt tacatcaata gcgcggtggg caactcaccg cgcctgggag
      781 aaagcggtgg ctagtaaacg ggaattcacg cttgaggaag cccagcgcat cggcgatcaa
      841 atcggcgtcg actggagcca atatgatctg gagcagtttc ggatgggcct agccgttgaa
      901 ctggaacacg gcgtccacga tctgcaaacg aacgtgaccg gcgacgatga gcttgtcacc
      961 ggcaagatcg ccctggccca tttgaaggag atccgcgact actactcccg cctcgccgtc
     1021 atggagcgcg aggcggaggc ctactgggcc acccaggaca agtagctgcg tccgcccggc
     1081 tttgcccggg ccaccggccc gatggagccc catcgggaat aaactggacg cccgatgggg
     1141 gcctatcggg aataaactag accagtgatc tggccaccgg cccgatatgc tccatgaact
     1201 ccgcccgcgt cttggggttg tcggcgaagg tgcccagcaa ggcgctggtg cgcatgcggg
     1261 cattggactt cttcacgccg cgcatggcca tgcacatgtg caggccatcg acgaccacgg
     1321 cgacgcccaa cggctgcacc agctcatcga tcagttcagc gatctgccgc gtcatgcgct
     1381 cctgaatctg caagcgacgg gcatagacct cgacgatgcg cgggatcttc gacaggccaa
     1441 tgaccttgcg gttggggata taggccacgt atgcccggcc gaagaaaggc agcatgtggt
     1501 gctcgcacag gctgtagaag tcgatgtcgc tgacgacgac catctcgctg taactggcct
     1561 cgaacagggc gttgttgagc aactccacgg gatcgacgcg gtagcccagc agcagctcat
     1621 cgtaggcgcg ggcgacgcgg ttgggcgtgt cccgcaggcc ctcccgctgc gggtcttcgc
     1681 cgacggcgcg caggatggtg cgcacagagt cggccacttt ctccttgcac gatgccgcca
     1741 gcaccgggcc gtcggtcaat tcgcccccgg gatcgggcgc ggtgttgtca aggtgtcttg
     1801 catctatcat aatctggaca ttatatcata gtggccagtg atcggtcgcc agtggccgcc
     1861 agaccgcaga ggttttctga aagctctggg ggctcgctga ggtttggcca gtggctactc
     1921 tacaggaggc tcgccatgaa ccgtgaccag caagaccagg ccgccgacgc ccgtttcgac
     1981 gcccgccgcg ccgcgcgcca ggcccacgcc gacaagtccg ccaagggcat cgtcgacttc
     2041 gatgagatgg tcaagctcga cgcccgccgc gagatgatcc atcccagcga cggcctcggc
     2101 ctggagcgca tcctgggcac aagcgacctg ctggagatca acttcct
//
LOCUS       DAOSMQ010000530         2136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_51709_length_2136_cov_3.923594, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000530 DAOSMQ010000000
VERSION     DAOSMQ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51709_length_2136_cov_3.923594"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PK829_13780"
     CDS             <1..1243
                     /locus_tag="PK829_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU42331.1"
                     /translation="AKETVLRRDVLIAVGEALAATRGEPRDPETPVGRLLAWRQTQLA
                     ANRERYSSHLYSRSDESALRVPEVKPIYPDNARLVPGAQILNAAFDAMLARDPRVVAF
                     GEDVGLLGGVNQTWAGLQKKYGPLRVSDTGIREATIIGQAIGLALRGLRPIAEIQYLD
                     YLLYGLQILSDDLASLHWRTRGGQKAPVIISTRGHRLEGVWHAGSPMAAVINLLRGLH
                     ICVPRDMTQAAGFYNTLLRGDEPGLVVEVLNGYRLKEHLPANIGDITVPLGVPEVLRE
                     GDDITLVTYGAACRIAVEAAEALAAVGIAVEIIDVQTLLPFDRYGHIVESLRKTNRVV
                     FLDEDMPGGATAYMLREVLETQGGFQWLDAEPRTISAQPHRPAYGSDGAYFSKPNAED
                     VFAVLYDMMNEANPTRFPSFL"
     gene            1361..>2136
                     /locus_tag="PK829_13785"
     CDS             1361..>2136
                     /locus_tag="PK829_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOU42332.1"
                     /translation="MTIQITLPEMGEGVIEGTLARWLVREGDRVERYAPIAEVETDKV
                     TTETTTEAAGTILRLCVSEGDTVPVGTVLAIIGEPGETTDDGQPPTAEKTTADRRPPT
                     AEKTAAEKTTAAPVADSLPAAPYTGRISPVVGRIAAEHGVDLGRVTGTGRDGRITKDD
                     ILAYVAGTTADGRPPTAASEAPTADGRPLTAATGASGRTTDESSLSDESPLAAVGGRP
                     SAAGKPSPTGESELLPLTNIRRAIAEHMVRSKHTSPHVTTV"
BASE COUNT          367 a          762 c          698 g          309 t
ORIGIN      
        1 ggccaaagag acggtgttgc gccgggacgt gctcatcgcc gtgggcgagg cgctggcggc
       61 cacgcggggc gaaccgcgcg acccggagac gcccgtcggc cggctgctcg cctggcggca
      121 gacgcaactg gcggccaacc gcgagcgcta tagctcccac ctctacagcc gttcggacga
      181 gtcggctctg cgcgtgccgg aagtcaaacc catctacccg gacaacgccc gccttgtccc
      241 cggcgcgcaa atcctcaacg ccgccttcga cgccatgctg gcccgcgatc cgcgcgtggt
      301 cgccttcggc gaggacgtgg gcttgctggg cggtgtcaat cagacctggg ccggcttgca
      361 gaagaagtac ggccccctgc gcgtctccga cacgggcatc cgcgaggcca ccatcatcgg
      421 ccaggccatt gggctggccc tgcgcggcct gcgccccatc gccgaaatcc agtatctcga
      481 ctacctgctc tatggcctgc aaatcctgtc cgacgacctg gcctccctcc actggcgcac
      541 acgcggcggg cagaaagcgc cggtcatcat cagcacccgc ggccaccggc tggagggcgt
      601 ctggcacgcc ggttcgccca tggccgccgt catcaaccta ttgcgtggcc tgcacatctg
      661 cgtgccacgc gacatgaccc aggccgccgg cttctacaac acgctgttgc gcggcgacga
      721 gccgggtctt gtcgtcgagg tgctcaacgg ctaccgcctc aaggagcacc tgccggccaa
      781 catcggcgac attactgtgc cgctaggcgt gcccgaagtg ctgcgcgagg gtgatgacat
      841 caccctggtg acctatggcg cggcctgccg catcgccgtc gaggcggccg aggccctggc
      901 cgccgtcggc atcgccgtcg agatcatcga cgtgcagacg ctgctgccct tcgaccggta
      961 cggtcacatc gtcgaatcgc tacgcaagac caaccgcgtc gtcttcctgg acgaagacat
     1021 gcccggcgga gcgacggcct acatgctgcg cgaggtgctg gagacgcagg gcggcttcca
     1081 atggctcgac gccgaaccgc gcaccatcag cgcccagccc caccgtccgg cctatggctc
     1141 cgatggggcc tacttctcca agcccaatgc cgaggatgtc ttcgccgtgc tctacgacat
     1201 gatgaacgaa gcaaacccga cgcggtttcc cagctttcta taatatgttg cgccttgagt
     1261 ttacccgcgg gggagcaggt ggacagggga gcagggggga gaagaggtct cccctgctcc
     1321 cccgctcccc tgctcccctg tccttctgtg gagaaccaca atgaccatcc aaatcaccct
     1381 gcccgaaatg ggcgaaggcg tcatcgaggg cacattggcc cgctggctgg tgcgcgaggg
     1441 cgaccgcgtg gagcgctacg cgcccatcgc cgaggtcgag acggacaagg tgacgaccga
     1501 gacgacgacg gaggcggccg ggacgatcct acgtctctgc gtgagcgagg gcgacaccgt
     1561 ccctgtcggc acggtgctgg ccatcattgg tgagccgggc gaaacgacgg atgacggcca
     1621 accgccgacc gccgagaaga cgaccgccga ccgccgaccg ccgaccgctg agaagacggc
     1681 tgctgagaag acgaccgccg cgccggtggc tgattcgctg cccgcggccc cgtataccgg
     1741 ccgcatcagt cccgttgtcg gccgcattgc cgccgagcat ggcgtcgatc tgggccgggt
     1801 gacgggcacg gggcgcgatg ggcggattac gaaggacgac atcctggcct atgtggccgg
     1861 gacgacggcc gacggccgac cgccgaccgc cgcaagcgaa gcgccgacgg ccgacgggcg
     1921 accgctgacc gccgcgactg gggccagtgg gcgaacgacg gatgaaagct ctctgtctga
     1981 cgaatctcct ctggcggcgg tcggcggtcg gccgtcagcg gccggcaagc cgtcgcccac
     2041 cggcgaatcg gaactactcc ccctgaccaa catccgccgc gccatcgccg agcacatggt
     2101 gcgcagcaag cacaccagcc cccacgtgac gacggt
//
LOCUS       DAOSMQ010000531         2124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52093_length_2124_cov_5.324795, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000531 DAOSMQ010000000
VERSION     DAOSMQ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52093_length_2124_cov_5.324795"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PK829_13790"
     CDS             complement(<1..464)
                     /locus_tag="PK829_13790"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOU42333.1"
                     /translation="MMIRRVAPPPDSQRGYAAGFVTRYIAFLIDIALVAGVALLTVTV
                     ARTTLAFFGFDQLLDYLFAPLDLEARSQVLSGAARWGITVGGGFLGFGVYSALAWMLV
                     GKTVGKALLGLRVLGRDGRRLTWRQAIVRSLAYYVSALPLFLGYLWVLVDDER"
     gene            complement(454..1248)
                     /locus_tag="PK829_13795"
     CDS             complement(454..1248)
                     /locus_tag="PK829_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42334.1"
                     /translation="MNDARPTHEPRIVEGVLVPTSTPLPAAGAPLSVTDPEFDAYMTI
                     VKALLGGAIEGTAELARRLERLEAEMRAAEGGPPGPGTVDSSADVVRYMLGGMVLSAA
                     DGARQRAVRLARTSDEFLRLTGSTIRPLANNRLTGIIVRPIDRAFGRLVARGQRRVDG
                     WIALGQTHEPSARLLARQAYLDILDDFIGHLSENEQLTTLVQEQSVGLANEFVEEVRE
                     RTVSADTLAESVVRRILRRPPRTALPPPEPIRAAVAEVKWVPRHDD"
     gene            1411..>2124
                     /gene="argS"
                     /locus_tag="PK829_13800"
     CDS             1411..>2124
                     /gene="argS"
                     /locus_tag="PK829_13800"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU42335.1"
                     /translation="MLHHHELAAAVHAALLAAQGDGTLPPFDLPAVTVERPRETSHGD
                     YATAVALQLARPARMAPLKIAAAIADHLPPIDYLDGATVAPPGFINFRLSPAWLQQLP
                     ERILAAPRGFGHNDLGDGKKAQVECVSANPTGPITLGRTRGGVIGDTLHRVLASAGYD
                     VTLEYYYNDAGRQITLLGESVKVRYLQLLGQPAELAEEHYQGEYIVELARELIAERGD
                     ALRDVPADTFAVYARDRISR"
BASE COUNT          377 a          767 c          673 g          307 t
ORIGIN      
        1 cgctcgtcgt ccaccagaac ccacagatag cccagaaaga ggggcagggc ggagacgtag
       61 taggccagcg agcggacaat ggcctgccgc cacgtcaaac ggcggccgtc gcggcccagc
      121 actcgcaacc cgagcagcgc cttgccaact gtcttgccga ccagcatcca ggccagggcg
      181 gagtagacgc cgaagcccag gaacccgccg ccaactgtga tcccccagcg cgccgcgccg
      241 gacagcacct gcgaacgcgc ctccaggtct agcggcgcaa agaggtagtc cagcaactgg
      301 tcgaagccga agaaggccag cgtcgtgcgc gccaccgtca ccgtcagcag agcgacgccg
      361 gcaacgaggg cgatgtcgat caggaaggcg atatagcgcg tcacaaagcc ggcggcatag
      421 ccgcgctgcg aatcgggcgg cggggcgacg cgcctaatca tcatggcgcg gcacccactt
      481 cacctcggcc acagccgcgc ggatcggctc cggcggcggc agcgccgtgc gcggcggccg
      541 gcgcaggatg cgccgcacaa ccgactcggc cagtgtgtcg gccgagacgg tgcgctcgcg
      601 cacctcctca acgaactcat tggccaggcc gacgctctgc tcctgcacca gggtagtcaa
      661 ctgctcattt tccgacagat ggccgataaa gtcatcgagg atgtccagat aggcctgccg
      721 ggccagcaag cgagcgctcg gctcgtgggt ctggcccagg gcgatccagc catccacccg
      781 ccgctggccg cgggcgacta ggcggccaaa ggcgcggtcg atggggcgga cgatgatgcc
      841 ggtcaggcga ttgttggcga gaggccggat ggtgctgccg gtcaggcgca ggaactcgtc
      901 cgacgtccgc gccagccgga ccgctctttg ccgcgcgccg tcggcggccg agagaaccat
      961 cccgccgagc atgtagcgca ccacgtcagc cgagctatcg acagtgccgg ggccgggcgg
     1021 gccaccctcg gcggcgcgca tctccgcctc caaccgctcc agccgccggg ccagctcggc
     1081 cgtgccctcg atggctccgc ccaatagcgc tttgacgatg gtcatgtagg cgtcgaattc
     1141 gggatcggtg acggacagcg gcgcgccggc cgcgggcagg ggcgtgctcg tcgggacgag
     1201 tacgccttcg acaatgcggg gttcgtgagt tggtctggcg tcgttcatag gtcgatgtgc
     1261 gcagcaatgt ctttgcgtcg atactcggtc tgtgccggca acaaccatat taccagcact
     1321 gcatttacag tataaaagac gggccaacaa tggtcaatcg acccatttcc ctcttcgact
     1381 gtccaccacc atactgttag aatctccatc atgcttcatc atcacgaact ggctgccgct
     1441 gtccacgccg cgcttctggc cgcgcaaggc gacggcacgc tgcctccctt cgacctgccc
     1501 gcagtgaccg tcgaacgccc gcgcgagaca agtcacggcg actacgccac ggccgtcgcc
     1561 ctgcaactgg cccgcccggc gcgcatggcc ccgcttaaga tcgccgcggc catcgccgac
     1621 cacctgccgc ccattgacta cctcgacggc gctactgtcg ccccgccggg cttcatcaac
     1681 ttccgcctgt cgccggcctg gctccagcag ttgccggagc gcatcctggc cgccccgcgc
     1741 ggctttggcc acaacgacct gggcgacggc aagaaggccc aggtcgagtg tgttagcgcc
     1801 aaccccaccg ggccgatcac cctcggccgg acgcgcggcg gtgtcatcgg cgacaccctc
     1861 caccgtgtgc tggccagcgc cggctatgac gtgaccctgg agtactatta caacgacgcc
     1921 ggccgacaga tcaccctgct gggcgaatca gtcaaggtgc gctacctcca actgctgggc
     1981 cagccggccg agctggccga agaacactac cagggcgagt acatcgtcga actggcccgc
     2041 gagctgatcg ccgagcgcgg cgacgccctg cgcgacgtgc ccgccgacac cttcgccgtc
     2101 tatgcccgcg accgcatctc ccgc
//
LOCUS       DAOSMQ010000532         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52137_length_2123_cov_3.771277, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000532 DAOSMQ010000000
VERSION     DAOSMQ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52137_length_2123_cov_3.771277"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PK829_13805"
     CDS             complement(<1..437)
                     /locus_tag="PK829_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010558703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42336.1"
                     /translation="MNCPRCRRLVPANTAFCVYCGAPLTPPSARRWLLPAVFLTVGLV
                     TAALVYTFWQLGPGRAGARNAALSDATLPPPPTAAAPSAAPSLATTTTTSTPLAATPA
                     PLSGVTSSLPDEIVFQSNRDGDYDIYIMALDGTNQRALTDNDV"
     gene            complement(504..1157)
                     /locus_tag="PK829_13810"
     CDS             complement(504..1157)
                     /locus_tag="PK829_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU42337.1"
                     /translation="MNNLFDRMRSGAEKAAFEADKLRRLSAAQSDLRGLRDEMNKAVN
                     QIGRIAFDLHQRGQGGPPELRAACEAATAVLAQISTQEAEIERIRNEQFLEHGADAKD
                     PSPLVCPVGHGPLAAGARYCQQCGQPGVPVAPVALIPCPTCGAALQPDARFCYNCGQP
                     MPAADAAPQPPAAPARSETVRLDSTKPVAACPSCGTPVLDASESFCSTCGYPFKRAT"
     gene            complement(1162..1770)
                     /locus_tag="PK829_13815"
     CDS             complement(1162..1770)
                     /locus_tag="PK829_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU42338.1"
                     /translation="MAMVTCPNCGHANAETARFCARCGHKLSPAETMVMGGFDDSIPL
                     PGTRPPTMSDKEQAAFNPPPPNSPETPIAPVPLRPVSAPPPVYTSPPPAVGAPVAANE
                     PPASSHRFVAMRTIAGLANVLAIVSLVLVGLGGLLVGYQMVSVLFGSSILGALGGLII
                     GAIAGALGWVYWRLLGEGIWVILDIESNTRRTAAALEGQRQP"
BASE COUNT          352 a          655 c          762 g          354 t
ORIGIN      
        1 ttgacgtcgt tgtcggtcag cgcgcgctga ttagtgccgt cgagggccat gatgtagatg
       61 tcgtagtcgc cgtcgcggtt gctctggaag acgatctcgt cggggaggga ggaggtcact
      121 ccggacaggg gcgcaggcgt ggcggccagg ggcgtgcttg tcgtggtggt ggtggcgagg
      181 gaaggcgcgg cgctgggagc ggctgctgtg ggtgggggcg gcagcgtggc gtcgctgagc
      241 gccgcattcc gcgcaccggc acgccccggc cccagttgcc aaaaggtgta gaccagcgcc
      301 gcagtgacca gccccacggt gaggaacaca gccggcagca gccagcggcg cgccgagggt
      361 ggcgtcagcg gcgcgccgca gtagacgcaa aaggctgtgt ttgccggaac cagacggcgg
      421 cagcgagggc agttcatcgc ctatgtcgcc ggccgctttg tcgctgtggg actatgcata
      481 tggaacctgg ctcctcttgt ggctcaggtt gcacgtttga aggggtagcc gcacgtcgaa
      541 cagaacgact cgctggcatc gagcaccggc gtgccgcacg acgggcaggc ggccaccggc
      601 ttcgtgctgt cgagccgcac ggtttccgaa cgcgccggcg cggccggcgg ctgcggcgcg
      661 gcgtccgcgg cgggcatggg ctggccgcag ttgtagcaga agcgcgcgtc cggttgcaac
      721 gctgccccgc aggttggaca agggatcagg gcgaccggcg ccacgggcac acccggctgg
      781 ccacactgct ggcagtagcg cgccccggct gccaacgggc cgtggcccac cgggcacacc
      841 agtggcgatg ggtctttggc gtcggcccca tgctccagaa actgctcatt gcgaatgcgc
      901 tcgatctccg cctcctgcgt gctgatctgg gccagcacgg ccgtggcggc ctcgcaggcg
      961 gcgcgcaatt ccggcgggcc gccctggccg cgctggtgca ggtcgaaggc gatgcggccg
     1021 atctggttga cggccttgtt catctcgtcg cgcaggccgc gcaagtctga ctgggcggca
     1081 gagaggcggc ggagcttatc ggcctcgaag gcggccttct cggccccaga gcgcatcctg
     1141 tcgaaaagat tattcatttg gctatggctg ccgctggccc tctagcgccg ccgcggttcg
     1201 gcgcgtgttc gactcgatgt ctagaatcac ccagatgccc tcgcccagca gccgccaata
     1261 gacccagccc agcgcgccgg cgatcgcgcc gatgatcagg ccaccgagcg cgccgagaat
     1321 gctgctaccg aataagacgc taaccatctg gtagccaaca agtaacccgc cgaggccgac
     1381 caataccagc gacacgatgg ccaggacatt ggccagaccg gcgatggtgc gcatggccac
     1441 gaagcgatga ctggaggccg gcggctcgtt ggcggccacg ggcgcgccga cggccggagg
     1501 cggcgacgtg tagaccggcg ggggcgcgga cacggggcgg agcggcacag gggctatggg
     1561 tgtctcaggg gagtttggcg gcggaggatt aaaggccgcc tgctctttgt cgctcatggt
     1621 cggcggccgg gtgcccggca gggggatgct atcgtcgaag ccgcccatca ccatcgtctc
     1681 cgccggcgat agcttgtggc cacaccgggc gcagaacctg gccgtttcag cgttggcatg
     1741 accacagttg ggacacgtta ccattgccat cgtctcactc tccttgcggc tacacgattc
     1801 cagggcagtg ctcaacgttt ctgcgcgccg gcgaacgcgt cgcccctgcg ctcggtgaag
     1861 aggaagtcga tgtcctgagg ctacgcaggc tcatggcgtg agtccatcga tgtgaacggt
     1921 gatggtttgt ccggtagcgc cggcgcagct ggccaagtca atatcacgtg gggcgccgag
     1981 caaaatccac tgtgccgaaa attatagcgg cctgtccgga aaaggctaca gattggcttc
     2041 atgggacagg gcgcttcagt tgttaccaat tggcttgaga cctgaccggc tatagcaaag
     2101 tagcacagcc tgttaggctg tcg
//
LOCUS       DAOSMQ010000533         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52238_length_2120_cov_3.714286, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000533 DAOSMQ010000000
VERSION     DAOSMQ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52238_length_2120_cov_3.714286"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PK829_13820"
     CDS             complement(<1..570)
                     /locus_tag="PK829_13820"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOU42339.1"
                     /translation="MTRPELLTVAEALAIVLDSVAPLGSETVALLDSLGRVLAEPVVA
                     ADSLPPFANSSMDGYALRAADLAGAGPDTGVRLRVVADVAAGAPSPVTLTPGTAARIM
                     TGAPLPEGADAVVPVEDTDEPWRDRDRPLPETVTIWRAVGSGDYVRYPGEDIRAGQTV
                     LAAGHLVRPQEVGVLASLGIERVAVVRRPR"
     gene            complement(563..1174)
                     /locus_tag="PK829_13825"
     CDS             complement(563..1174)
                     /locus_tag="PK829_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU42340.1"
                     /translation="MKGYADAWETVCRYVGDRGLRRHMQAVSAAMRFYADRLGEDQEA
                     WATVGLLHDFDWEIHPGLDEHPMKGALILRAEGWDEETIRIILSHYTEGTGVERNKPV
                     DFALLACDEITGLIIAATLVKPSRDIADTSVASIRKKWKDRRFAAGVDRDHVAEVTAD
                     FSRVCFDGGLELWPHIENVLTAMQGAAAELELDGRLAVAEAHD"
     gene            complement(1171..>2120)
                     /gene="ricT"
                     /locus_tag="PK829_13830"
     CDS             complement(1171..>2120)
                     /gene="ricT"
                     /locus_tag="PK829_13830"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOU42341.1"
                     /translation="MATVLAPGETSDFIGIRFQKLGKLYHFKVGSHKDLVPGDYVVVE
                     TKRGKQLGQVIAFIPPDEVNRQKGLRAVQRRATPRDLVMKQVWEAKELDALITCREEA
                     SRANVRDAKFVKAEFSFDGSWLIISYTTENKKLDVRQVQAALGRILRTRVEMQQVGPR
                     DVAKVMGGYGACGIPRCCSTFLTEFSPISIRMAKEQGISLSPQEITGMCGRLRCCLVY
                     EYEQYVEAKKQLPKVGKVIGTPHGEGKVVDVRPLRDSVIVQVGEEYHEVTRAQLEPLE
                     ELEALKKKAEAGCSKHENGGCDCGARAAPAADEPPAEGE"
BASE COUNT          354 a          723 c          677 g          366 t
ORIGIN      
        1 gcgcggccgc cgcacaacgg ccacacgctc gatacccagc gaggccagca cgcccacttc
       61 ctggggccgg acaaggtgcc cggctgccag caccgtctgc ccggcgcgga tgtcttcgcc
      121 aggatagcgc acgtagtcgc cggagccaac ggcgcgccag atggtaacgg tttccggcag
      181 cgggcggtcg cggtcgcgcc acggctcatc ggtgtcctcg acaggcacaa cagcatcagc
      241 gccttcaggc aggggcgcgc cggtcatgat gcgcgccgcc gtgcccggcg tcagggtcac
      301 gggtgatggc gcgccggcgg ccacgtcggc caccacgcgc agacgcacgc cggtgtctgg
      361 ccccgcgccg gccaggtcgg cggcgcgcag agcgtagccg tccatcgacg agttggcgaa
      421 ggggggcagg ctgtcggcag cgacaaccgg ttcagccaga acgcggccga gcgaatccag
      481 caacgccacc gtctcgctgc ccagcggggc aacgctgtcc agaacaatag ccagcgcctc
      541 ggcgacggtc agcagctcgg gcctagtcat gcgcctcggc gacagccagg cggccgtcta
      601 actccagctc ggccgccgcg ccctgcatgg ctgtcaggac gttctcaatg tgcggccaca
      661 gttccaggcc gccatcgaag cagacgcggc tgaagtcggc cgtcacctcg gccacgtgat
      721 cgcgatccac tcccgcggca aagcggcgat ccttccactt cttgcggatc gacgccaccg
      781 aggtgtcggc aatgtcgcgc gagggcttga ccagcgtggc cgcgatgatc aagccggtga
      841 tctcgtcgca ggccaggaga gcgaaatcga ctggcttgtt gcgctcgacg ccggtgcctt
      901 cggtgtagtg ggacaggatg atgcgaatcg tctcctcgtc ccaaccctcg gcccgcaaga
      961 tgagcgcgcc tttcatggga tgctcgtcca ggccggggtg aatctcccag tcgaagtcgt
     1021 ggagcaggcc aacagtggcc caggcctcct ggtcttcgcc cagccgatcg gcatagaagc
     1081 gcatggccgc gctgacggcc tgcatgtggc gacgcaggcc gcggtcgccc acgtagcggc
     1141 agacagtctc ccaagcgtcg gcgtagcctt tcattccccc tccgccggcg gctcgtccgc
     1201 ggccggggct gccctggccc cacagtcgca gccgccattt tcgtgcttgg agcagccggc
     1261 ctcggccttt ttcttcaggg cttccaactc ttccagcggc tctagctggg cgcgcgtcac
     1321 ctcatggtac tcctcgccaa cctggacgat gaccgagtcg cgcagcggcc gcacatccac
     1381 gaccttgcct tcgccgtggg gcgtgccgat gaccttgccg accttgggca gttgcttctt
     1441 tgcctcgacg tactgctcgt actcatagac caggcagcag cgcaggcggc cgcacatgcc
     1501 ggtgatctcc tgcgggctga gcgagatgcc ctgctccttg gccatgcgaa tagaaatggg
     1561 actgaattcg gtcaggaagg tggagcagca gcgcgggatg ccacaggccc catagccgcc
     1621 catgaccttg gccacatcgc gcgggccgac ctgctgcatc tcaacgcgcg tgcgcaggat
     1681 gcggccgagg gccgcctgca cctgacgcac gtcgagcttc ttgttctctg tcgtatagga
     1741 gatgatcagc catgagccgt caaagctgaa ttcggcctta acgaacttag catcgcggac
     1801 atttgcccgc gatgcttctt cgcgacaggt aatcagcgcg tccagttctt tcgcttccca
     1861 gacctgcttc atgaccaggt cgcgcggcgt cgcccgccgc tggacagcgc gcaggccctt
     1921 ctgccggttc acctcgtcgg gcgggatgaa ggcgatgacc tggccgagtt gtttgcctcg
     1981 tttcgtttcg acgacgacgt agtcgccggg aacgaggtct ttgtggctac cgaccttaaa
     2041 atgatacagc tttccaagtt tctgaaagcg aatcccaatg aagtcagagg tttcgccggg
     2101 cgccaacaca gtggccatag
//
LOCUS       DAOSMQ010000534         2119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52258_length_2119_cov_4.961725, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000534 DAOSMQ010000000
VERSION     DAOSMQ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52258_length_2119_cov_4.961725"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PK829_13835"
     CDS             complement(<1..1107)
                     /locus_tag="PK829_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HOU42342.1"
                     /translation="MTNLINQTLNSRYRLEALLGDGGMGTVYRAADLNLERQVAIKLM
                     HSHFARLEEFRARLVQEARTAAQLDHPSIVRVYDFGDSELGLFIAMEYVDGGSLRDHL
                     RRLQRLQKYLPLAQCLQIGIQMAEALDYAHRRKIIHRDVKPGNIILKRLSRPDEAGAQ
                     PFRALLTDFGLVKLQEGLSLTQSGATVGTPTYMSPEQCEGRELDGRSDLYSLGVVLYE
                     LVANRLPFTFQTLSEAIAAHQNNVQPALASTSRPEVPPIIDTLLARSMAKSPAQRFAT
                     GAEMADALRSAFVALEGLPTRVMLHQELDILDQVAEPPAGFELQIQTPGHGDSLVPLT
                     RAVITLGRNLDNDIVLPADGVSRHNWVLAGDAAHS"
     gene            complement(1117..>2119)
                     /locus_tag="PK829_13840"
     CDS             complement(1117..>2119)
                     /locus_tag="PK829_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008314890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOU42343.1"
                     /translation="ARVGDDVYGEDPTINALEALAAARVGKEAGLFVASGTMGNLTAV
                     LTHAGRGDEAILGADSHVYCWEAGGMAALGGVMPLALPTDSIGRLDLGAVEDAIREDD
                     PHMPRSRLILVENSYGGKHGYPLPPDYFAGIAAIARRHGLATHMDGARLFNAAVAQGI
                     SAADITAHVDSVTFCLSKGLCAPVGSVLCGSAAFIHQARRARKALGGGMRQAGILAAA
                     GVVALNEMIDRLAEDHAHARLLAEGLARLPGARVDVDKVRTNMVFFDLADDVPLSSEQ
                     VAGQLRETAGVWLGTNGPRGFRAVTHYWISAGDVGVLLDGLERILARAQEPAAPLTA"
BASE COUNT          353 a          731 c          695 g          340 t
ORIGIN      
        1 gctgtgggcg gcatcgcccg ccagcaccca gttgtggcgc gagacgccgt cggccggcag
       61 gacaatgtca ttgtcaaggt tgcggccgag ggtgatgacc gcccgcgtca ggggcacaag
      121 gctgtcgccg tggccgggcg tctgaatctg taactcgaag ccggccggcg gctcggccac
      181 ctggtcgagg atgtccaact cctgatgcag catcacccgc gtgggcagcc cctccagagc
      241 gacaaaggcg ctacgcaggg cgtcggccat ctccgcgccg gtggcgaagc gctgggcggg
      301 cgacttggcc atcgaccgcg ccagcagcgt gtcgatgatg ggcggcactt ccggccgcga
      361 cgtgctggcc agcgccggct ggacgttgtt ctgatgggcg gcaatagcct cggagagcgt
      421 ctggaaggta aagggcagcc tgttggctac cagctcatag aggacaacgc cgagcgagta
      481 gaggtccgag cgaccgtcaa gctcgcgccc ctcgcactgc tccggtgaca tgtaggtcgg
      541 cgtgcccacc gtcgcgccgc tctgcgtcag gctcaggccc tcttgcagct tcaccaggcc
      601 aaagtcggtc agcagggcgc ggaacggctg cgcgccggcc tcgtcgggcc ggctgagccg
      661 cttcagaatg atattgcccg gcttgacatc gcggtgaatg atcttgcgcc ggtgggcata
      721 gtcgagcgcc tcggccatct ggatgccgat ttgcagacat tgggccagcg gtaagtactt
      781 ctgcaaacgt tgcaggcgac gcagatggtc gcgcaggctg ccgccatcga catactccat
      841 ggcgatgaac agccccaact ccgaatcgcc gaaatcgtag acgcggacga tagaagggtg
      901 gtcgagctgc gccgccgtgc gcgcctcctg caccagccgc gcgcggaact cctccaggcg
      961 ggcaaagtgg ctatgcatga gcttgatggc cacttgcctc tccagattga ggtcagcggc
     1021 gcgatagacc gtgcccatgc cgccatcgcc cagcagggcc tccagacggt agcgactgtt
     1081 cagggtctga ttgatcaggt tggtcatcca tccctcctag gccgtcaacg gcgcggccgg
     1141 ctcctgcgcc cgcgccaaaa ttctctccaa cccgtccagc aataccccta cgtccccggc
     1201 cgaaatccag taatgagtca ccgcccggaa gccgcgcggg ccgtttgtgc ccaaccagac
     1261 gccggccgtc tcgcgcagct ggccggccac ctgctccgac gacagcggca cgtcatcggc
     1321 caggtcaaag aagaccatat tggtgcgcac cttgtccacg tccacgcgcg cgcccggcag
     1381 ccgggccagt ccctcggcca gcagccgcgc gtgggcatgg tcttcggcca gccggtcaat
     1441 catctcgttc agggcgacga cgccggccgc ggccagtatg cctgcctggc gcatgccgcc
     1501 gcccagcgcc ttacgcgccc gccgggcctg gtggatgaac gccgctgagc cgcacagcac
     1561 cgaccccacc ggggcgcaca ggcccttgct gaggcagaag gtcaccgaat cgacgtgggc
     1621 cgtgatgtcg gccgcgctga tgccctgggc tacggcagcg ttaaacaggc gcgcaccgtc
     1681 catatgggtg gccaggccat gtcgccgggc aatggcggca atgccggcga agtaatcggg
     1741 cggcagcggg tagccgtgct tgccgccgta gctattttct accaggatca gccggctgcg
     1801 gggcatgtgg ggatcgtctt cgcggatagc gtcttcgacc gcacccagat caaggcggcc
     1861 gatactgtcg gtcggcagcg ccaaaggcat caccccgcct agcgcggcca tgccgcccgc
     1921 ctcccaacaa tagacgtggc tgtcggcccc cagaatggcc tcgtcgcccc gcccggcgtg
     1981 ggtcagcacg gctgtcaggt tgcccatcgt gccgctggcg acgaatagtc cggcctcttt
     2041 gcccacccgc gccgcggcca gcgcctccag tgcgttgatg gtcgggtctt ccccatatac
     2101 atcgtcgccg acacgggca
//
LOCUS       DAOSMQ010000535         2119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52260_length_2119_cov_4.219961, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000535 DAOSMQ010000000
VERSION     DAOSMQ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52260_length_2119_cov_4.219961"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..1489
                     /locus_tag="PK829_13845"
     CDS             365..1489
                     /locus_tag="PK829_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42344.1"
                     /translation="MDIINQRPGLAALVAFIAGLIIGLVVLGWGLFPVQYTGTDPSGL
                     FPEYQAVFIRNAADLYSFDHDQEKLRSTLSGWGGDALACELASTSTDAGDQARLVAAA
                     TIVNGQGCLGLAAAPVATVPGAVLPTVPGQETPATEQGSSNLLPLLLGGLLLFLLLLA
                     LLYVWNRRRTLAEGEEPGATTYSQPSPVARSPRPAMKSAAATTTPVAPMAEAMAVPIA
                     RFRTAYTRGHDTYDDAFSIENANGDFLGECGVGISETIGATAPKNVTAFEVWLFDKND
                     IRTITKVIMSDHAFFDDALKAKLAPKGEPVLARENETIVLETATLLINAEITEMEYGQ
                     ASELPDTSYFERFTIELSAWAKEGETAMPNPGEGEDLLRY"
     gene            complement(1550..>2119)
                     /locus_tag="PK829_13850"
     CDS             complement(1550..>2119)
                     /locus_tag="PK829_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOU42345.1"
                     /translation="LADEIAYDAHDLDDGLRANLFTLEDLDELAIWRELCDSAGWTPG
                     THFSSLHRHEIIRELIGRSVTDVMIHTRAELAAAGIDSPPKLQLHPRNVVGYSPAFGD
                     KVRALKSFLYARMYRHYRLVRMQTKAERFIVGLFEAYMKEPDMLPRETWQRLERAPRA
                     RVVADYIAGMTDRYALDEWQKLYDPYMRA"
BASE COUNT          390 a          687 c          690 g          352 t
ORIGIN      
        1 tggcgcgggt ggcaggccct ccacgtcctg ggccgccgcc gcgccaacga acgtcgattc
       61 gatgttgacg atcgccccgc ggaagtcgcc ggagaggacg tactgcttgt cgccggtctg
      121 gccggccgcc ggttggtcag gttctgtcat ggcgcctggt gatagtatac aaggaacgtc
      181 aagacattgt atcccggtga gagttatggt aagatcattg ccagaagagg ggatgtcgcc
      241 taagagcgga tccggaacac gcggccgagg ccggcaagac gtgcgcggga agccgggcgc
      301 gccggaatag accgtgggga cgggttcgac aaatctaatc tatagatgca ggtaggtgac
      361 aaaaatggac atcatcaatc aacgccccgg cttggcggca ctggtagcat tcatcgcggg
      421 gctgatcatc ggccttgtgg tgttgggctg gggactcttc ccggttcagt acacgggcac
      481 agatccctca ggactcttcc cggagtacca ggcggtcttc atccgcaatg ccgccgatct
      541 ctactcgttc gaccacgatc aggaaaagtt acgctcaacc ctcagcggct gggggggtga
      601 cgcgctggcc tgcgagctgg caagcacctc caccgacgcg ggcgaccagg cgcgtctcgt
      661 cgccgcggcc actatcgtca acggccaggg ctgcctcggt ctggccgccg cgcccgtggc
      721 cacggtgccc ggcgcggtcc tgccgaccgt gcccggccag gaaactccgg cgaccgaaca
      781 gggcagttcg aacctactgc ccttactcct gggagggctg ctgcttttcc tgctgctgct
      841 ggccctcctc tacgtctgga accgccggcg gacgctggcg gagggtgagg agccgggcgc
      901 gacgacctat agccagccgt cgcccgtggc caggtcgcca cggccggcga tgaagtccgc
      961 cgcggcgaca accacgccag tggcccccat ggccgaagcc atggctgtgc ccatcgcccg
     1021 cttccgcacg gcctataccc gcggccacga cacctacgac gacgccttca gcatcgagaa
     1081 cgccaacggc gacttcctgg gcgagtgcgg cgtaggcatc tccgagacca tcggcgcgac
     1141 cgcgcccaag aacgtgacgg ccttcgaggt ctggctgttc gacaaaaacg atatccgcac
     1201 catcaccaaa gtgatcatga gtgaccacgc cttcttcgac gacgcgctca aggccaagtt
     1261 ggcccccaag ggcgagccgg tgctggcgcg tgagaatgag accatcgtcc tggagacggc
     1321 aacgctgctc atcaatgcgg agatcacgga gatggagtat ggccaggcgt ccgaactgcc
     1381 cgatacgagc tatttcgagc gcttcaccat tgagctgtcc gcctgggcca aggaaggcga
     1441 gacggccatg cccaaccccg gcgaggggga ggacttgctg cgctactaag ccggatcgtc
     1501 accggtcagt gggcgcggcg cggccgcgcc cactgaccgg tttatacccc taggcccgca
     1561 tgtacgggtc atacagcttc tgccactcat cgagcgcgta gcggtcggtc atgccggcga
     1621 tgtaatcggc cacgacgcgg gcgcgcggcg cgcgctccaa acgctgccac gtctcccgcg
     1681 gcaacatgtc cggctccttc atgtacgcct cgaatagacc aacgatgaag cgctcggcct
     1741 tggtctgcat gcgcaccagc cggtagtggc gatacatgcg cgcgtagagg aagctcttca
     1801 gggcacgcac cttgtcgcca aacgccggcg agtagccgac gacgttgcgc gggtgaagct
     1861 ggagcttcgg cggcgagtcg atgcccgcgg ccgccagttc agcgcgggtg tggatcatca
     1921 cgtcggtgac agagcggccg atcagttcgc gaatgatctc atgccggtgc agcgacgaga
     1981 agtgcgttcc cggcgtccag ccggcgctgt cgcacaactc gcgccagatg gccaactcat
     2041 ccaaatcttc cagcgtgaag aggttggccc gcaagccgtc gtcgaggtcg tgggcatcgt
     2101 aggcgatctc gtcggccag
//
LOCUS       DAOSMQ010000536         2118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52296_length_2118_cov_3.886573, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000536 DAOSMQ010000000
VERSION     DAOSMQ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52296_length_2118_cov_3.886573"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..354)
                     /locus_tag="PK829_13855"
     CDS             complement(43..354)
                     /locus_tag="PK829_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42346.1"
                     /translation="MRNRRLPLLIALALIGATLIWAGVARAQDTAAPPPTPAAAPIHP
                     VFPLLDDAGDNVLDSGRAISTMTTCGGCHDTAFIAANFYHADAGRATFGERGAAAGGH
                     A"
     gene            complement(402..1826)
                     /locus_tag="PK829_13860"
     CDS             complement(402..1826)
                     /locus_tag="PK829_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HOU42347.1"
                     /translation="MKQFRYLWVIGLVVTALIIAVPVLLFASSGNEASPDPWAYVAPT
                     PSHTNHAALLPGPYATGQEVTAACLECHETAAQEMMQTTHWTWESEPVLLPGRDEPVT
                     IGKKNQINNFCIGIQGNWTGCTKCHAGYGWDSASFDFAEQTNVDCLVCHADPGLYGKG
                     TAGLPADGVDLAAAAGSVRRPTRQNCGSCHFNGGGGNAVKHGDMDESLYYPDETLDVH
                     MGRYDFECVDCHAGDNHQILGHSISVSSEVKASALACTDCHVEPLHADARITTHTDAI
                     ACQTCHIPEGALRHATKVDWDWSTAGQDLPEDPHVYLKIKGNFVYEHDYRPIYAWWNG
                     AADRYIVGDLIDPTQVTNINTPLGDVSDVTAKIYPFKIHTARQPYDVLNQYLLVPMTA
                     GEGGFWDTFDWDSAFRLNEVNSGLAFSGEYGFAPTQMYWSMDHMVQPKENALQCYDCH
                     GENGRMDWEALGYNGDPMEWGGRE"
     gene            complement(1823..>2118)
                     /locus_tag="PK829_13865"
     CDS             complement(1823..>2118)
                     /locus_tag="PK829_13865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU42348.1"
                     /translation="CQALGDPKRLLILYALAERPRHVSALAADLDLPQPTISRHLQVL
                     RDRHLVTTARDGAAVVYSLAEPRVIGVLDTMRLILRDSLARQSGYLASAAEPL"
BASE COUNT          337 a          664 c          749 g          368 t
ORIGIN      
        1 tacaatcggg ttccagcggc cgaagatgcc ctgcccggtg tctcaggcat gcccgccggc
       61 cgccgcgccg cgctcgccga aggtggcgcg gcccgcgtcg gcatggtaga agttggcggc
      121 gatgaaggcc gtgtcatggc agccgccaca ggtggtcatc gtcgagatgg cccggcccga
      181 gtcgagcaca ttgtcgcccg cgtcgtcgag cagcggaaag accgggtgga tgggcgcggc
      241 cgccggcgtg ggcggcggcg cggccgtgtc ctgtgcccgg gccaccccgg cccagatgag
      301 cgtcgcgccg atcagggcca gagcaatgag taaaggaagt cgcctgttgc gcatggcgtg
      361 tatcctgctg cgtgaggtca atgagtggcg gtggggatcg tctactcccg gccgccccac
      421 tccatcgggt cgccgttgta gcccagggcc tcccagtcca tgcggccgtt ctcgccgtgg
      481 cagtcgtagc actgcaaggc gttctccttg ggctgcacca tgtggtccat cgaccagtac
      541 atctgcgtcg gcgcgaagcc atactcgccg ctgaaggcca ggccgctgtt cacctcgttc
      601 agccggaagg ccgaatccca gtcgaaggtg tcccagaagc caccctcgcc ggcggtcatg
      661 ggcaccagca gatactggtt gaggacatcg tagggctggc gggcggtatg gatcttgaac
      721 gggtagattt tggccgtcac gtcgctaaca tcgcccagcg gcgtgttgat gttagtcacc
      781 tgcgtcgggt cgataaggtc gccgacgatg tagcggtcgg ccgcgccgtt ccaccaggcg
      841 tagatcgggc gatagtcgtg ctcatagacg aagttgccct tgatcttcag gtagacgtgc
      901 gggtcttcgg gcaggtcctg gccggccgtc gaccagtccc aatcgacctt cgtcgcgtgg
      961 cgcagcgcgc cttcggggat gtggcacgtc tggcaggcaa tggcgtcggt gtgggtggta
     1021 atgcgcgcgt cggcgtggag tggctcaacg tggcagtcgg tgcaggccag ggccgacgcc
     1081 ttgacctcgc tcgacaccga aatgctgtgg cccagaatct ggtggttgtc gccggcgtgg
     1141 caatcgacgc actcaaagtc gtagcggccc atatggacat cgagggtctc gtcggggtag
     1201 tagaggctct cgtccatgtc gccgtgcttg acggcattgc cgccaccgcc attgaagtgg
     1261 caactgccac agttctgccg cgtcggccgg cgcacactgc ccgccgccgc ggccagatcg
     1321 acgccatccg ccggcaggcc cgccgtcccc ttgccgtaga ggccgggatc ggcgtggcag
     1381 accaggcaat cgacgttcgt ctgctcggca aagtcgaagc tggcgctgtc ccagccatag
     1441 ccggcgtggc atttggtgca gcccgtccag ttgccctgga tgccgatgca gaagttgttg
     1501 atctggttct tcttgccgat cgtcactggc tcgtcgcggc cgggcagcag caccggctcg
     1561 ctctcccacg tccagtgggt cgtctgcatc atctcctggg cggccgtctc gtggcactcc
     1621 aggcaggcgg ccgtcacctc ctgcccggtc gcataggggc cgggcagcag cgcggcgtgg
     1681 ttggtgtgcg atggcgtcgg ggcgacgtag gcccagggat cgggcgacgc ctcattgccc
     1741 gacgaagcga acagcagcac gggcacggcg atgatcagcg ccgtgaccac gaggccgatg
     1801 acccagaggt agcgaaattg cttcatagtg gctccgcggc cgaggcaagg tagcccgatt
     1861 ggcgggcaag gctatcgcgc aagatgaggc gcatggtgtc caaaacaccg atgacgcgcg
     1921 gctcggccag tgaatagacc actgctgcgc cgtcgcgcgc ggtcgtcacc aggtggcggt
     1981 cgcgcaagac ctgcaagtgg cgggagatgg ttggctgcgg cagatcgagg tcggccgcca
     2041 acgccgagac gtggcgcggc cgctcggcaa gggcgtagag gatgagcaac cgcttggggt
     2101 cacccagggc ctgacaga
//
LOCUS       DAOSMQ010000537         2110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52556_length_2110_cov_5.139659, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000537 DAOSMQ010000000
VERSION     DAOSMQ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52556_length_2110_cov_5.139659"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PK829_13870"
     CDS             complement(<1..703)
                     /locus_tag="PK829_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018524745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU42349.1"
                     /translation="MTIGDVSMERRQRLDSLQFHESVLTTIGNTPLVRLNAVTGSSEA
                     LVLAKLEFFNPGGSVKDRIGWPIIEDAERDGRLRPGGTIVEATSGNTGVGLAIAAALK
                     GYRCIFVMPDKMSQEKILLLRAFGARVVVTPTAVEPDDPRSYYSVARRLVEETPNAIL
                     ANQYHNPINPQAHYEMTGPEIWAQTDGLITHFIAGMGTGGTITGVGRYLKERNPAIQV
                     VGVDPERHFRFIEPRR"
     gene            868..>2110
                     /locus_tag="PK829_13875"
     CDS             868..>2110
                     /locus_tag="PK829_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU42350.1"
                     /translation="MKRSRLTVVVAVLVLMLITVVVVSAATYIVQPGDTLWRISRQFG
                     TTVEAIVQANNIPNPNLIYVGQTLEIPVGPTAVPPTAQPPGPTTTPQPPQPTPTSSPT
                     TTYVVQPGDTLTAIAARFGTTVQAVVQANNIVNPNLIFVGQVLVIPGTTVVPPAPTVA
                     PTVPGATPQPPSGFAFGGQTQTFANAQAMTGTGMNWVKFQYKWSAGDDTSDVAALIQS
                     AKNAGFKVLISVTGETIYPAANSLDFGALTQFMRDLAALGPDAIEVWNEQNIDFEWPA
                     GQIDPTSYVNNMLAPAYQAIKAANPNVMVISGAPAPTGFDNGTNAWADNRYIAGMRAA
                     GAANYADCIGVHYNAGATSPTVSTGHPGGSHYSWYYQPMVDLYYNTFGGARQLCFTEL
                     GYLSGEGFSGLPANFAWASGTS"
BASE COUNT          384 a          678 c          647 g          401 t
ORIGIN      
        1 gccggcgtgg ttcgatgaag cgaaagtggc gctccggatc gacgccgacg acctgaatgg
       61 ccgggttacg ctccttcaga tagcggccga cgccagtgat cgttccacct gtgcccatgc
      121 cggcgatgaa gtgggtgatg aggccgtccg tctgtgccca aatctccggg ccggtcatct
      181 cgtagtgggc ctgggggttg atcgggttgt gatactggtt ggccaggatg gcattgggcg
      241 tctcctcaac gaggcgacgg gcgacggagt agtagctacg cgggtcatcg ggttcgacgg
      301 ccgtcggcgt gacgacaacg cgcgcgccga aggcgcgcag cagcaggatt ttctcctgcg
      361 acatcttgtc gggcatgaca aagatgcagc gatagccctt cagcgccgcg gcgatggcca
      421 ggccgacgcc ggtgttgccg gaagtggcct caacgatggt gccgccgggg cgcaggcggc
      481 cgtcgcgctc ggcgtcctcg atgatcggcc agccgatgcg atccttgact gagccgccgg
      541 ggttgaagaa ctctagtttg gccaacacca gcgcttcact gctgccggtg acggcgttga
      601 gccgcaccag cggcgtgttg ccaatggtcg tcagcaccga ttcgtggaac tggaggctat
      661 ctagtctctg gcggcgctcc atactgacat cgccaatcgt catatgctct cacctgaggc
      721 cggaattcac gctcgcttca tagctatgct accagcaaat cgggcatgat gcatgcagca
      781 agttatgtaa aaaaggcttg cgattatgta acgttatgtc tataatgtta ttgtgtgccc
      841 tttgaccgtt agcgaaggag tgaagccatg aaaaggtcgc ggcttactgt ggtcgtggca
      901 gtgctcgtct tgatgctcat caccgtcgtt gtcgtctcgg cggctaccta catcgtccag
      961 ccgggcgaca ccctttggcg catctcgcgc caattcggga cgacggttga ggccatcgtc
     1021 caggccaaca atattcccaa ccccaacctg atctacgtcg ggcagacgct ggagatcccc
     1081 gttgggccga cagctgtgcc gcccacggcg cagcctcccg ggccgacgac cacaccgcag
     1141 ccgccacagc caacgccaac ttcgtcgccc acgacaacct acgtcgtcca gccgggcgac
     1201 acgctgacgg ccattgccgc gcgctttggc acaacggtcc aggccgtggt gcaagccaac
     1261 aacatcgtta accccaacct gatcttcgtc ggtcaagtgc tcgtcattcc gggcacgacc
     1321 gtcgtgccac cggcccccac ggtcgcgccg accgtgcccg gggcgacacc acagccgccc
     1381 agtggcttcg cctttggtgg ccagacacag accttcgcta acgcgcaggc catgaccggc
     1441 acgggcatga actgggttaa gttccagtat aaatggtcgg ccggcgacga cacttctgac
     1501 gtggccgccc ttattcagtc ggccaagaac gccggcttta aagtgttgat ctccgtcact
     1561 ggcgagacga tctatccggc ggccaacagc ctcgactttg gcgcgctgac ccagttcatg
     1621 cgcgacctgg ctgcgcttgg ccccgacgcc attgaggtct ggaacgagca gaatatcgat
     1681 ttcgagtggc cggcagggca gatagacccg acgtcctacg tcaataacat gctcgccccc
     1741 gcctaccagg ccatcaaggc ggctaacccc aatgtgatgg tcatctccgg cgcgccggcg
     1801 ccgacgggct tcgacaacgg cacgaacgcc tgggccgaca accgctacat cgccggcatg
     1861 cgggccgccg gggcggccaa ctatgccgac tgcatcggcg tccactacaa cgccggcgcg
     1921 acttcgccga ctgtgtccac cggccatccg ggtgggtcgc actatagctg gtactatcag
     1981 ccgatggtcg atctttacta caacaccttc gggggcgcgc ggcagttgtg cttcaccgag
     2041 ctgggctatc tgtcgggcga gggcttcagc ggcctgccgg ctaacttcgc ctgggcctct
     2101 ggtacgtcgg
//
LOCUS       DAOSMQ010000538         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52618_length_2108_cov_5.864101, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000538 DAOSMQ010000000
VERSION     DAOSMQ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52618_length_2108_cov_5.864101"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PK829_13880"
     CDS             <1..876
                     /locus_tag="PK829_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU42351.1"
                     /translation="LDNFNDYYDPARKRANEQRLCAYPNFRMIEADVRDRATLFGLFE
                     DARFDAVAHLAAMAGVRDAVKQPDLYVNVDLNGTQNLLDAARAAGNVGNFVMASTSSV
                     YGNTTRQPFIETDPCDRPLQPYAAAKRAAEILGHSYYYLFGQNFIALRFFTVYGPNGR
                     PDMMAYLVADSITKGKQIPLYDGGQMYRNWTYVDDTVAGVAAALDRPLGYEIINLGQD
                     QPVLLADFVTLLEELSGRKANVVPTPRLAADVTSNEADISKARQLLDYNPRVPVSEGV
                     RRFWEWYRHYDLGQP"
     gene            857..2026
                     /locus_tag="PK829_13885"
     CDS             857..2026
                     /locus_tag="PK829_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOU42352.1"
                     /translation="MTSASHSPDAETDKPLAPFRLVADAAAWDVCLADLRGMSQLAID
                     LEANSMFAYSERACLIQLSTASTDYIIDPLAGLDLSGLGAALADPTVEKIFHAAEYDL
                     ILLRRDYDWSLVNLFDTMWAARILGYRQMGLAGLLEAFFGVRLSKRYQKANWCHRPLS
                     TPELAYAQKDTHYLPALRDRLAAELEERGLMAEALETFAEQARVRLPNNGFDPEAFWN
                     INGVQDMTPPQQAAMRALYLFRDREARRRNAPHFKVLSDRTLLEIAAALPARMSELDA
                     IHGMTSGQQQRYGRQLLEAVAEARQSPPPQPPKRPPRLPDAVLIRYDRLHRWRKARAQ
                     ARGVESDVIVSRDALWAIAHANPHTAGELAALDVLGPWRLERYGQEILRELGVRN"
BASE COUNT          387 a          724 c          651 g          346 t
ORIGIN      
        1 ctggacaact tcaatgacta ctatgacccg gcgcgcaagc gggccaacga gcagcggttg
       61 tgtgcctacc ccaactttcg catgatcgag gccgacgtgc gcgaccgggc aacgctcttc
      121 ggcctcttcg aggacgcgcg cttcgacgct gtggcgcacc tggcggctat ggccggggtg
      181 cgcgacgccg tcaagcagcc agacctgtac gtcaacgtag acctgaacgg cacgcagaac
      241 ctcctcgacg cggcgcgggc ggccggcaac gtgggtaact tcgtcatggc ttcgacctcg
      301 tcggtctatg gcaatacgac gcgccagccg ttcatcgaga ccgacccctg cgaccggccc
      361 ttacagccct atgccgcggc caagcgcgcc gccgaaatcc tgggccactc ttactactac
      421 ctcttcggcc agaacttcat cgccctgcgc ttcttcactg tctatggccc caacggccgg
      481 ccggacatga tggcctatct ggtagccgac agcatcacca agggcaagca gatccctctc
      541 tacgacggcg ggcagatgta tcgcaactgg acgtatgtcg atgacaccgt cgccggcgtc
      601 gccgccgcgc tcgaccggcc gctgggctac gagatcatca acctgggtca ggatcagccg
      661 gtgttgctgg ccgacttcgt gaccctgttg gaagagttga gcgggcgcaa agccaacgtt
      721 gttcccacgc cgcggctggc tgccgacgtg accagcaacg aggccgacat cagcaaggcc
      781 cgccaactgc tggactataa cccgcgcgtg cccgtcagcg agggcgtgcg ccgcttctgg
      841 gaatggtatc gtcactatga cctcggccag ccatagcccc gacgcggaga ccgataagcc
      901 cctggctccg tttcgtctgg tggccgacgc cgctgcctgg gacgtctgcc tggccgacct
      961 gcgcggcatg tcgcagttgg ccatcgacct ggaggccaac agcatgttcg cctacagcga
     1021 gcgggcctgc ctcatccagc tttcgacagc ctcaaccgac tacatcatcg acccgctggc
     1081 tggccttgac ctgagcgggc tgggcgcggc gctggccgac ccgaccgtgg agaaaatctt
     1141 ccacgccgcc gagtatgacc tgatcctgct gcggcgtgac tacgactgga gcctagtcaa
     1201 tctgttcgac acgatgtggg cggcgcgcat cctcggctac cggcagatgg ggctggccgg
     1261 gctgctggag gcgttcttcg gcgtgcggct cagcaaacgc taccagaaag ccaactggtg
     1321 tcaccggccc ctctcgaccc ccgaactggc ctatgcccag aaggataccc actacctgcc
     1381 ggccctgcgc gaccggctgg cggccgagct ggaggaacgc ggcctgatgg ccgaggcgct
     1441 ggagaccttc gccgagcagg cgcgcgtgcg gctgcctaac aacggtttcg accccgaagc
     1501 cttctggaac atcaacggcg tccaagacat gacgccgccg cagcaggcgg ccatgagagc
     1561 gctgtacctc ttccgcgacc gcgaggctcg ccgccgcaac gcgccccact tcaaagtgct
     1621 gagcgaccgc acgctgctgg agatcgccgc tgccctgccg gcgcgtatgt cggaactgga
     1681 cgcgattcac ggcatgacca gcggccagca acagcgctat ggccgccaac tgctggaggc
     1741 tgtagccgaa gcgcgccagt cgccgccgcc gcaaccaccc aagcgcccgc cacgcttgcc
     1801 cgacgccgtc ctcatccgct atgaccggct gcaccgctgg cgcaaggcgc gcgcccaggc
     1861 tcgcggcgtc gaatccgacg ttatcgtcag ccgcgacgcc ctgtgggcta ttgcccacgc
     1921 caacccgcac acggccgggg agctggcggc gctggacgta ctcggcccgt ggcggctgga
     1981 gagatatggc caggagatac tacgagaatt aggagttagg aattaggaat tgggaagata
     2041 gccacaggga atacgctctt cgattcgtaa ttcctaattg aggaaggagt tgcccatgga
     2101 aatcacat
//
LOCUS       DAOSMQ010000539         2102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52839_length_2102_cov_3.783097, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000539 DAOSMQ010000000
VERSION     DAOSMQ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52839_length_2102_cov_3.783097"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK829_13890"
     CDS             <1..268
                     /locus_tag="PK829_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU42353.1"
                     /translation="ALGIFFGAAVPAGWSLDFALAVTFIGIVVPALRDRPHVAAALSA
                     GLVAVLTFGWPYKLGLMAAALTGILAGVALESRGGSRVRQEAQP"
     gene            265..588
                     /locus_tag="PK829_13895"
     CDS             265..588
                     /locus_tag="PK829_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOU42354.1"
                     /translation="MSNITLILTIIGMGVITFAIRVSFFLLPEGVQLPERLLRALRYV
                     PAAVLSAIIAPELLLPSGAFDLSLGNERLLAGLVAILVAWRTRNVLLTVIAGMGALWA
                     LQALI"
     gene            781..>2102
                     /locus_tag="PK829_13900"
     CDS             781..>2102
                     /locus_tag="PK829_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU42355.1"
                     /translation="MTINSDTRAIEAQIDARIEARLEYWIEQLGRLCAQPSISAQRLG
                     IDDCAALVATMLREEGYTAEVLPTGGSPIVYGDSGPRDGAPTLLFYNHYDVQPPEPLE
                     LWNSPPFMLTRAGDHLYARGVSDDKGQLITRMAAAAAVRDVLGELPCRVKFFVEGEEE
                     IGSPNIPPAIDAHKERFAADACIWEFGSVDYTGRPTQTLGMRGLAYFELSAETASQDC
                     HSGLGGSLIANAAWRLVWALSTIKGVDERIRIPGFYDKAHPATERDLAYLAGLPDDSE
                     AFKASYGVTGFLKGMASGTELRRAAVFEPTATINGLTSGYQGPGAKTVLPARASAKLD
                     FRLVPDQTPEEIGPLLRAHLDAEGFPDVEVRYLAGVKPARTDPDHPFVQLSIRAAEDV
                     YGKPPSVEPLIGGSGPLHPFIHVLGLPVVTSGCGYPESRVHAPNENIR"
BASE COUNT          317 a          750 c          679 g          356 t
ORIGIN      
        1 ggcgttgggc atcttcttcg gcgcggccgt gccggctggc tggtcgctcg acttcgcctt
       61 ggccgtaacc ttcatcggca tcgtcgtgcc cgccctgcgc gaccggccgc acgtcgccgc
      121 ggcgctctcg gccggcctgg tagccgtgtt gaccttcggc tggccctaca agctggggct
      181 aatggccgcg gcattgaccg ggattcttgc cggcgtggcg ctggagagcc gtggcggcag
      241 tcgcgtccgg caggaggcgc agccgtgagc aacataaccc tcatcctcac cattattggc
      301 atgggcgtaa tcacctttgc catccgcgta tcgttcttcc tgctgccaga gggcgtgcaa
      361 ctgccggagc ggctgctgcg cgccctgcgt tacgtacccg cggccgtcct ctcggccatc
      421 atcgccccgg agctactgct gcccagtggc gcgttcgatc tgtcgctggg caatgagcgg
      481 ctgttggccg ggctggtggc cattctcgtc gcctggcgca cgcgcaacgt cctgctgacg
      541 gtcatcgccg gcatgggcgc gctgtgggcg ctgcaagcgc taatctgatc cccctccctc
      601 cgggaggggt taggggaggg cgcggcggaa gaccctctcc ccaaccctct ccccaggggc
      661 gagggagtct tgtctccctc cgggaggggc taggggaggg ccgctccggc tcctttggcc
      721 cgcgctccac cttgcgctat agtcctcgac aagaccaccc atcccaatca tggagaccct
      781 atgaccatca acagtgacac ccgggccatc gaggcccaga tcgacgcgcg catcgaggca
      841 cgcctggagt actggatcga gcaactcggc cgcctttgcg cccagcccag catttctgcc
      901 caacgcctgg gtatcgacga ctgcgccgcg ctggtggcga cgatgctgcg cgaagaaggt
      961 tacactgccg aagtcctgcc cacgggcggc agtcccatcg tctatggtga cagtggcccg
     1021 cgcgacggcg cgccgacgct gctgttctat aaccactatg acgtccagcc gccggagccg
     1081 ctggaactgt ggaactcgcc gcccttcatg ctgacccgcg ccggcgacca cctctacgcc
     1141 cgcggcgtca gcgacgacaa gggccagctc atcacccgca tggccgccgc cgcggccgtg
     1201 cgcgacgtgc tgggcgagct gccctgccgc gtcaagttct ttgttgaggg cgaggaggag
     1261 atcggcagcc ccaacatccc gcccgccatc gacgcgcaca aggagcgctt cgccgccgac
     1321 gcctgcatat gggagttcgg tagcgtcgat tacactggcc ggccgacgca aacgctgggc
     1381 atgcgcggcc tggcctactt cgagctatcg gccgagacag cctcgcaaga ctgccactcc
     1441 ggcctgggcg gctcgctgat cgccaacgcc gcctggcggc tggtatgggc gctgagcacc
     1501 attaagggcg tcgatgagcg catccgcatt cctggcttct acgacaaagc ccacccggcc
     1561 accgagcgcg atctggccta tctggctggg ctgcccgacg actccgaggc gtttaaggca
     1621 tcctatggcg tgaccggttt cctgaagggc atggccagcg gcacggagct acgacgcgcg
     1681 gccgtgtttg agccgacggc gaccatcaat ggcctgacct ccggctatca ggggccggga
     1741 gccaagacgg tgctgcccgc ccgcgccagc gccaagctcg acttccgcct ggtgcccgac
     1801 cagacgccag aggagatcgg gccgctgctg cgcgcccacc tggacgccga gggcttcccg
     1861 gacgtggagg tgcgctacct ggccggggtc aagccggccc gcaccgaccc agaccatccc
     1921 ttcgtgcagc tatccatccg cgccgccgag gatgtctatg gcaagccacc cagcgtcgag
     1981 ccgctcatcg gcggcagcgg gccgttgcac ccgttcattc acgtcctggg cctgccggtg
     2041 gttacgtcgg gttgtggcta tccggaaagc cgcgtccacg cgcccaacga gaacatccgc
     2101 at
//
LOCUS       DAOSMQ010000540         2099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52921_length_2099_cov_5.038160, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000540 DAOSMQ010000000
VERSION     DAOSMQ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52921_length_2099_cov_5.038160"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PK829_13905"
     CDS             <1..1252
                     /locus_tag="PK829_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOU42356.1"
                     /translation="LGAEGYALTVAPDGARLSAHAPAGLFYGVQTLRQLLPPAIENAT
                     PLPGQGPLPGPWSLPAGRIRDVPRFAWRGLMLDVARHFFPVADVRRVIDLAAAYKINR
                     LHLHLSDDQGWRIDIASWPELARVGGQTAVGGGPGGYYTQADYAALVAYAAARHITIV
                     PEIDLPGHTNAALAAYPQLNADGRPRPPYTGIEVGFSSLAIDNELTYRFIDDVIGELA
                     ALTPGDYLHIGGDEALATAKEDYIRFIERVAAIVRAHGKRMVGWEEIGQARLEPSAIA
                     QHWASGHATAAAAQGASVILSPAPRVYLDMKYDAATPLGLAWAGHISVRAAYDWEPAT
                     QLAGVAEAQLLGVEAALWSETLTTLADIEFMLFPRLPGVAEVGWSAAGRDWETYRARL
                     AAHGPRLGAMGVNFYRAAEIDWSP"
     gene            complement(1436..>2099)
                     /locus_tag="PK829_13910"
     CDS             complement(1436..>2099)
                     /locus_tag="PK829_13910"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791602.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOU42357.1"
                     /translation="NARTPRVMGVLEPLKVIIENYPKGQEETFDVPYYKQDKTRTESR
                     PLPFSREIYIERDDFMEEPVKGFTRLAPEVEVRLMSAYYIVCTGVVKDSVGRIVELRA
                     IYDPATRGGTSADGRKVRGTLHWVSAEHALPAEARLFDYLFTKPDPQENGENFLDNIN
                     PDSLKIVDALVEPSLATAAPGDRFQFVREGYFCVDLDSTPDRLVFNRTVGLKDTWVKG
                     KE"
BASE COUNT          323 a          738 c          699 g          339 t
ORIGIN      
        1 cctgggcgcg gagggctacg ccctcaccgt cgccccggac ggcgcgcgcc tgtccgccca
       61 cgcgccggcg gggctgttct acggcgtcca gacgctgcgc cagctcttgc cgccggccat
      121 cgagaatgcc acgcccctgc ccgggcaggg gcccctgccc gggccatggt ccctgcccgc
      181 cgggcgcatc cgcgacgtgc cccgcttcgc ctggcgcggc ctgatgctcg acgtggcccg
      241 ccacttcttc cccgtggccg acgtgcggcg ggtcatcgat ctggccgcgg cctacaagat
      301 caaccggctg catctgcacc tgagcgatga ccagggctgg cgcatcgaca tcgcctcgtg
      361 gccggaactg gcccgcgttg gcgggcaaac ggccgtcggc ggcgggccgg gcggctatta
      421 cacccaggcc gactacgccg ccctggtcgc ctacgccgcc gcgcgccaca ttaccatcgt
      481 gccggagatc gacctgcccg gccataccaa cgccgccctg gccgcctacc cgcaactcaa
      541 cgccgacggt cggccccggc cgccctacac cggcatcgag gtcggcttca gcagcctggc
      601 catcgacaac gaactgacct accgcttcat cgatgacgtg atcggcgagc tggcggcact
      661 gacgccgggc gactatctcc atatcggcgg cgatgaggct ctggccacgg ccaaagagga
      721 ctacatccgc ttcatcgagc gcgtcgccgc catcgtccgc gcccacggca agcgcatggt
      781 cggctgggag gagatcggcc aggcgcggct ggagccgtcg gccattgccc agcactgggc
      841 ctccggccac gccaccgccg ccgccgcgca aggggcgagc gtcatcctct cgcccgcgcc
      901 gcgggtctac ctggacatga aatacgacgc ggcgacgccg ctgggcctgg cgtgggccgg
      961 ccacatcagc gtgcgcgcgg cctatgattg ggagccggcg acgcagctag ccggcgtggc
     1021 cgaggcgcaa ctgctgggcg tggaggccgc gctgtggtca gagacgctga cgacgctcgc
     1081 cgacatcgag ttcatgctct tcccgcggtt gccgggcgtg gccgaagtag gctggtcggc
     1141 cgccggccgg gattgggaga cataccgcgc ccgcctggcg gcgcatgggc cgcggctagg
     1201 ggcgatgggg gtcaacttct atcgcgcggc ggagatcgac tggtcgccct aagtcctgtg
     1261 gaactgtcac gcaaccacgg tttgagacgg ggcgagcttc aggacgcgcg tcgcgtttaa
     1321 gtggttggcg ggcagaaaat agggtcgtag ggcatctacg ccgtaagcca acttccatcc
     1381 agtaccaata gcaaaacggc cgggcgcaag cccggccgtt atcgtctatt ggggcctact
     1441 ctttgccctt aacccacgta tccttcaacc ccactgtccg gttaaacacc agccggtcgg
     1501 gcgtcgaatc gagatcgacg cagaagtagc cctcgcgcac gaactgaaat ctgtcgccgg
     1561 gcgcggccgt ggccaggctc ggttccacca gcgcgtcgac gatcttcagc gagtcggggt
     1621 tgatattgtc gaggaagttc tcgccattct cctgcgggtc gggcttggtg aacaggtagt
     1681 caaagaggcg cgcctcggcc ggcagagcgt gctcggccga gacccagtgc agcgtgccac
     1741 gcaccttgcg gccatcggcc gaggtgccgc cgcgcgtggc cgggtcatag atcgcccgta
     1801 gctcgacgat gcgcccgact gagtccttga ccaccccggt gcagacgatg tagtaggcgc
     1861 tcatcagccg cacttcgacc tccggggcca gccgggtgaa gcccttgacc ggctcctcca
     1921 tgaagtcgtc gcgctcgatg tagatttcgc gcgagaaagg caacggccgg gactcggtgc
     1981 gcgtcttgtc ctgcttgtag tagggcacgt cgaaggtctc ttcctgcccc ttggggtagt
     2041 tctcgatgat caccttgagc ggctccagca cgcccatgac ccgtggcgtg cgcgcgttc
//
LOCUS       DAOSMQ010000541         2097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_52995_length_2097_cov_4.061214, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000541 DAOSMQ010000000
VERSION     DAOSMQ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52995_length_2097_cov_4.061214"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PK829_13915"
     CDS             <1..366
                     /locus_tag="PK829_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOU42358.1"
                     /translation="YPTGEKQEINTPKFGQWYDWATVIAGDCHFIKRFMPPRLDGKII
                     CTNTTTAADVSAFRATGVRTLVTTTPRFEGRSFGTNMMEAMLVAVSGEGRKLTDAELR
                     AWADRLGFRPTVAELNPAG"
     gene            372..896
                     /locus_tag="PK829_13920"
     CDS             372..896
                     /locus_tag="PK829_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOU42359.1"
                     /translation="MSSKILVAYASRTGTTAGVAEVIGQVLAADGTAVDVRPVAEVNN
                     LSPYSAIVVGSAIQGAEWLPEAMQFIRDHQRELAARPFAAFLVCMTLAMTNRDYHDEV
                     ARWLQPVCDLVEPVSMGLFAGALDISKVPTLRARLNFRISVRLGVWSEGDHRDWAEIR
                     AWAEELRPLLAAEA"
     gene            899..2032
                     /locus_tag="PK829_13925"
     CDS             899..2032
                     /locus_tag="PK829_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HOU42360.1"
                     /translation="MTRVEQLLADLVAIDSTNPDLVPGAAGEGAIAAFVAEWLTAAGL
                     EVHVEEVRPGRPNVVGIARGQGGGRTLLLNGHIDTVGVAGMTAPHTPQVRDGRLYGRG
                     AYDMKGGVAACMVAAAEAARLDLRGDVIVTAVMDEEYAGLGTLAVAERTRADGAIVAE
                     PTEMALVVAHRGFAWLEVETRGVAAHGSRPHLGIDAIVKMGGVLAGLARLSRDLANGP
                     AHPLLGPASVHASLISGGQELSSYPERCLLSVERRTLPGETGATAAAELQAIVDELAA
                     ADPAFRAEVRLGLERAPMETPAGAAISAALQAAATAVLGQPRAPSGVSFWTDAASLHA
                     AGIPTALFGPAGEGAHAVEEWVDLASVRACAAVYLAVARAFCQ"
BASE COUNT          295 a          726 c          765 g          311 t
ORIGIN      
        1 tacccgacgg gcgagaagca ggagatcaac acgcccaagt ttggccagtg gtacgactgg
       61 gccacggtca tcgccggcga ctgccacttc atcaagcgct tcatgccgcc gcgcctggac
      121 ggcaagatca tctgcaccaa cacgaccacg gccgccgacg tgtctgcttt ccgcgccacg
      181 ggcgtgcgta ccctggtgac gacgacgccg cgcttcgagg gccgctcctt cggcaccaac
      241 atgatggagg cgatgctggt ggccgtcagt ggcgaggggc gcaagctgac cgacgccgag
      301 ttgcgtgcct gggccgaccg gctggggttc cggccgaccg ttgcagagtt gaaccccgcg
      361 ggataagaac catgtcaagc aagattctcg ttgcctacgc cagccgcacc ggcacaactg
      421 ccggcgtggc tgaggtcatt gggcaggtgc tggctgcgga cggcacggcc gtagacgtgc
      481 ggccagtggc cgaggtgaac aacctgtccc cctacagcgc cattgtcgtc ggcagcgcca
      541 tccagggcgc ggagtggctg ccggaggcga tgcagtttat ccgcgaccac cagcgtgagc
      601 tggcggcccg ccctttcgcc gccttcctgg tctgcatgac cctggccatg accaacaggg
      661 attaccacga cgaggtcgcc cgctggctac agccggtgtg cgacctcgtc gagccggtca
      721 gcatgggcct gttcgccggg gcgctggaca ttagcaaggt gccgaccttg cgggcgcggc
      781 tcaatttccg catcagcgtg cgcctgggcg tctggtcgga aggcgatcac cgtgactggg
      841 ccgagattcg cgcctgggcc gaagagctgc gccccttgct ggccgcggag gcgtaggcat
      901 gacgcgcgtc gagcaactgc tggccgatct ggtggccatc gattcgacta atcccgatct
      961 ggtccccggc gcggccggcg agggagctat cgccgccttc gtcgctgagt ggctgaccgc
     1021 cgccgggttg gaagtccacg tcgaggaagt gcgaccgggg cgtccgaacg tcgtcggcat
     1081 tgcccgcggc cagggcggcg gccggacgct gctgctcaac ggccacatcg acaccgtcgg
     1141 cgttgccggc atgaccgcgc cccacacgcc gcaggtgcgc gacggccgcc tctacggccg
     1201 cggcgcctac gatatgaagg gcggcgtggc ggcctgcatg gtcgccgcgg ccgaggcggc
     1261 ccggctcgac ttacggggcg acgtcatcgt cacggcggtg atggacgagg agtatgccgg
     1321 gctgggcacg ctggccgtgg ccgaacgcac tcgcgccgat ggggccatcg tcgccgaacc
     1381 gaccgagatg gccctcgtcg tcgcccaccg gggcttcgcc tggttagagg tggagacgcg
     1441 cggcgtggcc gcccacggct cgcggcccca cctcggcatc gatgccatcg tcaagatggg
     1501 cggcgtgttg gccgggttgg ctcgcctgag ccgcgacctg gccaacggcc cggcccaccc
     1561 gctgctcggc cccgcctccg tccacgcctc gctcatcagc ggcgggcagg aactatcgag
     1621 ctacccggag cgctgcctcc tgtccgtgga gcggcgcacg ctgccgggcg agacgggagc
     1681 cacggccgcg gccgagttac aggccatcgt cgatgagttg gcggcggccg accccgcctt
     1741 tcgggccgag gtgcgcctgg gcctggagcg cgcgccaatg gagacgcccg ccggcgcagc
     1801 catcagcgcg gcgctacagg cggcggcgac ggctgtgctg ggccagccgc gcgcgccgag
     1861 cggcgtgtcg ttctggacgg acgcggcctc gctccacgcc gccggcatcc ccacggcgct
     1921 cttcggcccg gccggcgagg gggcgcatgc cgtcgaggag tgggtcgatc tggccagcgt
     1981 gcgggcctgc gccgcggtct atctggccgt agcgcgggcg ttctgccagt agtggcggct
     2041 gaacgattgg ccacgcggtg gcgggcgctc tggccaggcg cggacgaggc cacggtc
//
LOCUS       DAOSMQ010000542         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_53681_length_2077_cov_6.210682, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000542 DAOSMQ010000000
VERSION     DAOSMQ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53681_length_2077_cov_6.210682"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PK829_13930"
     CDS             <1..700
                     /locus_tag="PK829_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOU42361.1"
                     /translation="VVIRCESSINYDWGGGGPGNGLGNDNFSVRWTGRFNFSGGNTRF
                     IAAADDGVRVWVDGALLIDGWRDQATTEYRGERSLSADIHEVKVEYYENGGGAVARLR
                     WEGAEGCPDNQYRAEYFNNRTLSGNPVVIRCESSINYDWGGGGPSNGLGNDNFSVRWT
                     GRFNFSGGNKSFIATADDGIRIWIDGTLLIDGWRDQAPTEYRAQRSLSAGVHEVKVEY
                     YENGGGAVARLRWE"
     gene            1116..>2077
                     /locus_tag="PK829_13935"
     CDS             1116..>2077
                     /locus_tag="PK829_13935"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU42362.1"
                     /translation="MPDLTLPGLIDVHTHLRVPGGEHKEDFATGTAAALAGGITLVLA
                     MPNTNPPLTTPEALAAARRAAAAGARCDVGLFAGAAPADVAHLPALAPDAAALKIYLN
                     DTFGRLRVDDLPTLVACFRDWPRDKMIALHAEKQSVAVAIGLAATYDRPAHFCHISRR
                     EEIELIADAKGRGLPITCEVTPHHLFLSQADAARLGPLGDMRPVLGTAGDVAALWAHL
                     GDTIDCIATDHAPHTRAEKASDAPPPGVPGLETSLPLLLSAVASGRLTTARLVALMHD
                     NPRRIFGLPPQPDTWVEVDMTPWTLPAEGWQTKCGWTPFAGLTAG"
BASE COUNT          363 a          692 c          692 g          330 t
ORIGIN      
        1 ggtcgtcatt cgctgcgagt cgagcattaa ctacgactgg ggtggtggcg gcccgggcaa
       61 cgggctgggc aacgacaact tctctgtccg ctggacggga cgattcaact tcagcggtgg
      121 caacacacga tttatcgctg cagctgacga tggcgtccgc gtgtgggtgg atggggcact
      181 gctcatcgac ggctggcgcg atcaggctac gaccgagtac cgcggcgagc gctcgctaag
      241 cgcggacata cacgaagtca aggtggagta ctacgagaac ggcggcggag ccgtcgcccg
      301 cttgcgttgg gagggggctg aaggctgtcc cgacaatcag tatcgggccg agtacttcaa
      361 caatcgcacc ctgagcggca acccggtcgt cattcgctgc gagtcgagca ttaactacga
      421 ctggggtggt ggcggcccga gcaacgggct gggtaacgac aacttctccg tccgctggac
      481 gggacgattc aacttcagtg gtggcaataa atccttcatt gccaccgccg acgacggcat
      541 ccgcatctgg atcgatggga cgttactcat cgacggctgg cgcgatcagg ctccgaccga
      601 gtaccgggcg caacgatcgc ttagtgctgg cgtgcacgaa gtcaaggtcg aatactacga
      661 gaacggcggc ggggctgttg cccggctgcg gtgggaataa gggttaggga tgggaaatag
      721 ttaaagtatg cttgtggact ttccccatat agtggagtca acaaggagga tagaaacgtt
      781 tctcaaaatc agctgggctt agggtagccc aatgccaaat ggagccacta tcgattgtag
      841 aacacctcca tggaatcgaa aatggctgca cgcgaggttt gttttcttgc cttcagcccc
      901 ttgcttctaa ccatgtctct gctatactga ttttccagtg tgcacgaggc gcgacagact
      961 gggggcgacg cgaggcgtcg cgacaacgag caaagccgcc ggcgacaagc cggccggcaa
     1021 ccccattctt gatcccccat cgcccgatcc gaccggatcg ggcttttttt tgcccgccgg
     1081 tggccgcgcc gattcccgcc ccacggacaa acgccatgcc tgacctgacc ctgcccggac
     1141 tgatcgacgt ccacacccac ctgcgcgtgc ccggcggcga gcacaaggaa gatttcgcca
     1201 cgggcacggc cgcggcgctg gccggcggca tcaccctggt gctggccatg cccaacacca
     1261 acccgcccct gaccactccg gaagcgctgg ccgcggcccg ccgcgctgcc gcggccggcg
     1321 cgcgctgcga cgtgggtctg ttcgccgggg ccgccccggc cgacgtggcc cacctgcccg
     1381 cgctggcccc cgacgccgcg gcgctgaaga tctatctcaa cgacaccttc ggccgcctgc
     1441 gcgtcgatga cctgcccacg ctggtggcct gcttccgcga ctggccgcgc gacaagatga
     1501 tcgccctcca cgccgagaag cagagcgtcg ccgtggccat cggcctggcg gccacctacg
     1561 accggccggc ccacttctgc cacatcagcc gccgcgagga gatcgagctg atcgccgacg
     1621 ccaaggggcg cggcctgccg atcacctgcg aggtgacgcc ccaccacctt ttcctgagcc
     1681 aggccgacgc ggcgcggctg gggccgctgg gcgacatgcg gccggtgctg ggcacggccg
     1741 gcgacgtggc cgccctgtgg gcgcacctgg gcgacaccat cgactgcatc gccaccgacc
     1801 acgcgccgca tacgcgggcc gagaaggcgt cggacgcgcc gccgccgggc gtgccggggc
     1861 tggagacgag cctgccgctg ctgctcagcg ccgtggccag cgggcggctg acgacggcgc
     1921 ggctggtggc cctgatgcac gacaaccccc ggcgcatctt cggcctgccg ccgcagccgg
     1981 acacgtgggt ggaggtggac atgacgccgt ggacgctgcc ggccgagggc tggcagacga
     2041 aatgcggctg gacgcccttc gccgggctga cggccgg
//
LOCUS       DAOSMQ010000543         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_53885_length_2072_cov_3.806148, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000543 DAOSMQ010000000
VERSION     DAOSMQ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53885_length_2072_cov_3.806148"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PK829_13940"
     CDS             complement(<1..702)
                     /locus_tag="PK829_13940"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOU42363.1"
                     /translation="MAMLTLSPREARRLAVAAQQLEAPRPNPTKADLLDMIRRITCVQ
                     IDPINVVARTQLLVPFSRLGPYDPADLETLLWDDKALFEYWAHAASIVLADDYPFFRP
                     QMGERLPGNGVWQQRVHAWWTANAAFRQRLLDELARRGPLFTEEFESRADVPWNYDNR
                     WGGSNDVNIILDMLWFRGSVTVTRRAGNGYGLKKQWGLLEHQLGDRVGAPEVARPVLV
                     RRAVERALLALGPARQ"
     gene            909..>2072
                     /locus_tag="PK829_13945"
     CDS             909..>2072
                     /locus_tag="PK829_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42364.1"
                     /translation="MKRPLAILLVLLALLALAACGGATPADPGADPGATTLPDQPTAA
                     VTIPAETPTAGLPTAEPPTAAPTAEPTATTATTPAALCPDLPRPALILSTGPGFEAHN
                     PLSGERCPLPLPETMGYLQPGGDHLYFVAFDSEASTNVVARLGADETVEPLEPTRATG
                     DVYYLLRFAVAADESHLAWSHMASQDAAATAMQGSLWIGTADGRAATPIFENQLGGEL
                     RIVTPLRFTADGATLYFTWEPIGLGGAWNSFNGRYDNLYRVPVAGGAPEKVFDCADAG
                     LFLCAGDFLDDGTVAYTDTSRIIHVAGPGGAELAAIPTTAEYAGYPTFSPTGDLYYSE
                     AVLPANNPNVAVPAPGTVYRLAAPYSGAPALVATADGLLPSATAQPFLDADHLV"
BASE COUNT          323 a          766 c          673 g          310 t
ORIGIN      
        1 ctggcgggcc gggccgaggg ccaacagcgc ccgctcgacc gcccgccgca ccaacaccgg
       61 tcgcgccacc tccggcgcgc ccacgcggtc gcccaactgg tgttctagca gtccccactg
      121 cttcttcagg ccgtagccgt tgcccgcccg ccgggtgacg gtgacgctgc cccggaacca
      181 cagcatgtcg aggatgatgt tgacatcgtt gctgccgccc caacgattgt cgtagttcca
      241 cggcacgtcg gcccggctct cgaactcttc ggtgaacaac gggccgcggc gggccagttc
      301 gtccagaagg cgctgccgga aggcggcgtt ggccgtccac caggcgtgga cgcgctgctg
      361 ccagacgccg ttgcccggca agcgctcgcc catctgcggc cggaagaagg gatagtcatc
      421 ggccaggacg atggaggccg cgtgcgccca gtactcgaac agcgccttgt cgtcccatag
      481 cagcgtctcc aggtcggccg ggtcatatgg ccccagacgg ctgaagggca caagcagttg
      541 cgtccgggcg acgacgttga tgggatcgat ctgcacgcag gtgatgcggc ggatcatgtc
      601 gagcaggtcg gctttggtgg ggttcggccg cggcgcttcc agttgttgcg cggccacggc
      661 caggcggcgc gcttcccgcg ggctgagggt gagcatggcc atcgtgggcg ggataatagc
      721 cgaacagatg tttcagggca agcgtggcgc gattctcccg aatcgcgtgc tgcgattcag
      781 gagaatcgcg ccacggtgta ctccaaatag accgccggct gtaacctatt tggcagacga
      841 ctctgccggc gtcccctaca ctaacgccca gtccaccgcg ctgaccaagc gcccgagaga
      901 gaaagacgat gaaacgacca cttgcgatcc tgctcgtttt gcttgccctg ctggctctgg
      961 ccgcctgcgg cggcgcgacg ccggctgatc ccggcgctga tcccggcgcg acgaccctgc
     1021 ccgaccaacc cacggccgcc gtgacgatac ccgcggaaac gcccaccgcc ggcctaccca
     1081 cggccgagcc acccacggcc gcccccacgg ccgaaccgac ggccaccacc gcgacgacgc
     1141 cggcggcgct ctgccccgac ctgcctcgcc cggccctgat cctcagcacc ggccccggct
     1201 tcgaggccca caacccgctc tccggcgagc gctgcccgct accgctgccg gagacgatgg
     1261 gctacctcca gcccggcggc gaccacctct actttgtcgc cttcgatagc gaggccagta
     1321 ccaacgtcgt cgcgcgcctg ggcgcggacg aaaccgttga gccgctggag ccgacgcggg
     1381 ccacaggcga cgtctactac ctgttgcgct tcgccgtggc cgccgacgag agccatctgg
     1441 cctggagcca catggcgtcg caggacgccg cggccacagc catgcaaggc agcctgtgga
     1501 tcggcacggc cgacggccgc gccgccacac ccatcttcga gaaccagctt ggcggcgagt
     1561 tgcgcatcgt cacgccgctg cgcttcaccg ccgacggcgc gacgctgtac ttcacctggg
     1621 agccgatcgg cctgggcggc gcgtggaatt ccttcaacgg ccgctatgac aacctctacc
     1681 gcgtgccggt cgccggcggc gcgccggaga aggtcttcga ctgtgccgac gcgggcctgt
     1741 ttttgtgcgc gggcgacttc ctcgatgatg gcaccgtggc ctacaccgac acgagccgga
     1801 tcatccacgt tgccgggcca ggcggggcag aactggccgc catcccgacg acggcggagt
     1861 acgccggcta tccgaccttc agccccacgg gcgacctgta ttacagcgag gcggtgctcc
     1921 cggccaataa ccccaacgtg gctgtgcccg cgccggggac ggtctaccgg ctggcggcgc
     1981 cctacagtgg cgcgccggcg ctggtggcca cggccgacgg tctgctgccc tcggccacag
     2041 cccagccctt cctcgacgcc gaccatctgg tt
//
LOCUS       DAOSMQ010000544         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_53891_length_2072_cov_3.169063, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000544 DAOSMQ010000000
VERSION     DAOSMQ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53891_length_2072_cov_3.169063"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..228
                     /locus_tag="PK829_13950"
     CDS             85..228
                     /locus_tag="PK829_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42365.1"
                     /translation="MRFFALQRELTELIGREVDLNTPGFLSPSFRDDVLAEAENLFSL
                     WAE"
     gene            424..666
                     /locus_tag="PK829_13955"
     CDS             424..666
                     /locus_tag="PK829_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42366.1"
                     /translation="MDKRFQPCAVIMHMRDGTTVEQVYPTRFQAELFLYMLPLLMSTQ
                     PHEQGVASAILSPLVEGRAYEPVSVPRPQRSDSYLQ"
     gene            907..1329
                     /locus_tag="PK829_13960"
     CDS             907..1329
                     /locus_tag="PK829_13960"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HOU42367.1"
                     /translation="MDPIATLRAAHEAFSRHDLPSIIRDMRRDVVVYGHAAGQRLPSA
                     DAFAQFMNMYFTMSSDIAIVDAEYFAHGNKVVAQFRAVGTQDGPFLGFPASDRPFTLD
                     VAEIWTFDDEGYAIEGHNYADTLGLLIQLGHVPAPALA"
     gene            1374..1646
                     /locus_tag="PK829_13965"
     CDS             1374..1646
                     /locus_tag="PK829_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42368.1"
                     /translation="MYALIRRQLNDYDRWKETVTTLDGLRAQYGSRGMTIFRAARDPN
                     EVYLLLEWDDDKSPDDYLNLPQVQQALASTGTSEVIAISEVMRLPY"
     gene            complement(1723..>2072)
                     /locus_tag="PK829_13970"
     CDS             complement(1723..>2072)
                     /locus_tag="PK829_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012161734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HOU42369.1"
                     /translation="VGQQFLRGYQLTDANGVAAFTTIYPGWYSGRTVHIHFKIRTEEG
                     GARYEFTSQLFFDDALTDSVFTRDPYAARPDRDTRNAADGIYRGGGDRLLLSPAASGE
                     GYAAVFDIGLAMG"
BASE COUNT          365 a          764 c          606 g          337 t
ORIGIN      
        1 cctatggccg gggctgttat ttctatcagc cgttgacaca gttgctgtca ggtcggcgcg
       61 ccacgtgccc gaccgcgcgt cggcttgcgc tttttcgccc tgcaacgaga actaaccgaa
      121 ctgatcggcc gcgaagtcga tctcaacacc ccgggcttcc tcagtcccag ctttcgcgac
      181 gacgtgctgg ctgaagcgga gaacctcttc tctctctggg cggaataacc gacccaccca
      241 cccgcttttc tggcgtttcc gccaatagac acacggccgc ggtgcgttat cctataccaa
      301 tcgttcgccc ccgtggccac gcctcccgtg gcccggccgc gggcttgcga gcgggcttcc
      361 cacgccgaag ccgaaccgaa ccttacgaac acagggggcg cgccgccccg ccgaggagaa
      421 gacatggaca agcgattcca gccctgcgcc gtcatcatgc acatgcgcga cggcacgacc
      481 gtcgaacagg tctacccgac ccgcttccag gccgagctgt tcctgtatat gctgccgctg
      541 ctgatgtcca cgcagccgca cgagcagggc gtcgccagcg ccatcctctc ccccctggtc
      601 gaagggcgcg cctatgagcc tgtgtccgtt ccccggccgc agcgcagcga tagctacctt
      661 caataggcgg ccaccagagg cggccaccag aggcggccac cagccacaac gtcaagacct
      721 gaccggtggg cggcgcgaag ccctatacct cccagcgcct cataccgccc accggtcagg
      781 tccggaacaa ctggcaactg accacacatt gagcaccttt tggtctgtcg gacactcccc
      841 gcgctctgct atgctacgcc catcatcccg ggctggacat gcccagacag aaaggagaat
      901 ttaccgatgg accctatcgc taccctgcgc gccgcccacg aggccttcag ccgccacgac
      961 ctgcccagca tcatccgcga catgcgccgc gacgtcgtcg tctacggcca cgctgccggc
     1021 cagcgcctgc cttcggccga cgccttcgcc cagttcatga acatgtactt cacgatgtcg
     1081 tccgacatcg ccatcgtgga cgccgagtac ttcgcccacg gcaacaaggt cgtcgcccag
     1141 ttccgcgccg tcggaacgca agacggcccc ttcctgggct tccccgccag cgaccgcccc
     1201 ttcacgctcg acgtggccga aatctggacc ttcgatgacg agggctacgc catcgaaggc
     1261 cacaactacg ccgacacgct cggcctgctc atccagctgg gccacgtgcc cgccccggcc
     1321 ctggcctgag ccgggccgcg ccgccccgtg agacagaacc aaaaggtgac actatgtacg
     1381 ctctcattcg ccgtcaactg aacgactatg accgctggaa agagaccgtc acgaccctcg
     1441 acgggttgcg cgcccaatat ggctcgcgcg gcatgaccat ctttcgcgcc gcccgtgacc
     1501 ccaacgaggt ctacctgctg ctggagtggg acgacgacaa gtcgcccgac gactacctga
     1561 acctgccgca ggtgcagcaa gccctcgcgt ccacgggcac gtcggaggtc atcgccatca
     1621 gcgaagtcat gcggctgccg tattagtggg ccgcaggcag ccgttccgtg ggccggcgat
     1681 ccgtggtctg gcggcggtcg ctggcccacc cacaacggcc agctacccca tcgccagccc
     1741 aatatcgaac acagccgcgt agccctcgcc gctcgcggcc gggctgagca gcagccggtc
     1801 gccaccgccg cggtagatgc cgtcggcggc gttgcgcgtg tcgcgatccg gccgcgcggc
     1861 ataggggtcg cgggtgaaga cgctgtcggt cagcgcgtcg tcgaagaaga gttgcgaggt
     1921 gaactcgtag cgcgccccgc cctcttcggt gcgaatcttg aagtggatgt gcaccgtccg
     1981 gccgctgtac cagccggggt agatggtcgt gaacgcggcc acgccgttgg cgtcggtcaa
     2041 ctggtagccg cgcagaaact gctgcccgac gg
//
LOCUS       DAOSMQ010000545         2069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_53983_length_2069_cov_3.314300, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000545 DAOSMQ010000000
VERSION     DAOSMQ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53983_length_2069_cov_3.314300"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2069)
                     /gene="priA"
                     /locus_tag="PK829_13975"
     CDS             complement(<1..>2069)
                     /gene="priA"
                     /locus_tag="PK829_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU42370.1"
                     /translation="AQMVAVPARVAGPVRRRPQLPPELAAALARLPADPEELPPEALA
                     ALLDAGHARLEAQPAAVSLLIPRRAVYSHILRLRRAETYGAILDLLAAEARAVEVGEI
                     YARTGATLKHLRRLADLDLVALKAAEVWRDSLADRDFAPAEPPELTLDQARVWGRIKV
                     AMIAGEDDDWDEDDAPATGAQPATTDDGADAHNLDSPTLDIDALDPMDAATPFLLHGV
                     TGSGKTEIYMRAIDFALERGQQAIVLVPEIALTPQTVRRFAARFPGRVAVLHSALGDG
                     ERYDTWRRARQGLFDIAIGPRSALFAPLPNLGVIIVDEEHDASYKQTPPVPPPYYHAR
                     DAAIALAALTNATVILGSATPDVVTYHRARSGRYQLLELPRRIMGHRQRIEGQAERLG
                     IASHYQPLTGGGDGEGRDVDDFLRLDDALTIPLPPVQLVDMRQELRANNRSIFSRALD
                     AAVTETLARGEQAILFLNRRGTSTFVNCRDCGHVMNCPRCGVPLTYHQPQLLLVCHQC
                     GRREPNPVVCPNCGSERIRYFGLGTEKLAELVAARWPEARLIRWDRDTTTDEGSHEAL
                     LAGFIERRADILVGTQMIAKGLDLPFVTLVGVISADISLGLPDYNTGERVFQVLAQVA
                     GRAGRGLLGGRVIVQTYQPEHYAIRTAAEHDYPSFYIEEIRFRTQRALPPFRRMARLL
                     IADPARY"
BASE COUNT          316 a          686 c          763 g          304 t
ORIGIN      
        1 gtagcgggcc gggtcggcaa tcagcagccg ggccatgcgc cggaagggcg gcagggcgcg
       61 ctgggtgcgg aagcgtatct cctcaatgta gaagctgggg taatcgtgct cggcggcggt
      121 gcgaatggcg tagtgctccg gctggtaggt ctggacgatc acgcggccgc ccaacaggcc
      181 gcgcccggcc cgccccgcca cctgggccag cacctggaag acgcgctcgc cggtattata
      241 gtcgggcagc cccagcgaga tgtcggccga aatgacgcca accaacgtca caaacggcaa
      301 gtccagcccc ttggcgatca tctgcgtgcc aacgaggatg tcagcgcggc gctcgatgaa
      361 gccggccagc agcgcctcgt gggagccttc gtccgtcgtc gtatcgcggt cccaccggat
      421 gagccgcgcc tctggccaac gcgccgcgac cagttcggcc agcttctccg tgcccagccc
      481 gaagtagcgg atgcgctctg aaccgcaatt ggggcagacg accgggtttg gctcacgccg
      541 gccgcactgg tggcagacca gcaacaattg cggctggtga taggtcaggg gcacgccgca
      601 gcgcgggcag ttcatcacgt ggccacagtc gcggcaattg acgaaggtcg atgtgccccg
      661 gcggttgagg aagaggatag cctgctcgcc gcgggccagc gtctctgtca cggcagcgtc
      721 gagcgcgcgg ctgaagatag agcggttgtt ggcgcgcagt tcctggcgca tatccaccag
      781 ctgcacgggc ggcagaggaa tggtcagggc gtcatcgagg cgcaggaagt cgtccacgtc
      841 gcgcccctca ccgtcgccgc cgccggtcag cggctggtag tggctggcga tgccgaggcg
      901 ctcggcctgc ccctcaatgc gctggcggtg gcccataatg cgccgcggca gttccagcag
      961 ttgatagcgg ccggagcgcg cccggtggta ggtgaccaca tcgggcgtgg cgctgccgag
     1021 gatgacggtc gcattggtca gggcggccag ggcaatggcc gcgtcgcggg cgtggtagta
     1081 gggcggcggc acgggcggcg tctgcttgta gctggcatcg tgctcctcat cgacgatgat
     1141 aacacccagg ttgggcagcg gcgcgaagag agcgctgcgc gggccaatag cgatgtcgaa
     1201 caggccttgc cgcgcccgcc gccaggtgtc atagcgctcg ccgtcgccca aggcgctgtg
     1261 gagcacggcc acgcggccgg ggaagcgcgc cgcgaaacgc cgcaccgtct gcggcgtcag
     1321 ggcgatctcc ggcaccagaa cgatggcttg ctggccgcgt tccagagcga agtcgatggc
     1381 ccgcatgtag atctcggtct tgccgctacc ggtgacgccg tggagcagaa agggtgtggc
     1441 cgcgtccatc gggtcgaggg cgtcgatgtc gagggtaggt gagtcgaggt tatgggcatc
     1501 cgcgccgtcg tccgtcgtgg ccggctgtgc gcccgtcgcc ggggcgtcgt cctcgtccca
     1561 atcgtcgtcc tcgccggcga tcatggccac tttgatgcgc ccccagacgc gggcctggtc
     1621 gagggtcagc tccggcggct cagccggagc aaagtcgcgg tcggccagcg agtcgcgcca
     1681 gacctcggcc gccttcagcg ctaccagatc gaggtcagcc agccgccgca ggtgcttcag
     1741 cgtcgccccc gtgcgggcgt agatctcgcc cacctccacc gcccgcgcct cggcggccag
     1801 caggtcgaga atcgcgccat aggtctcggc gcgacgcagg cgcaggatgt ggctgtagac
     1861 agcgcggcgg ggaatgagca ggctgaccgc ggctggctgc gcctccaggc gagcatgccc
     1921 ggcgtcgagc agggccgcca gcgcttcggg cggcaattct tccgggtcgg ccggcagccg
     1981 cgccagagcc gcggccagtt ccggcggcaa ctgtggccgg cggcgcaccg ggccggccac
     2041 gcgggcgggc acggccacca tttgcgccg
//
LOCUS       DAOSMQ010000546         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_54389_length_2058_cov_3.213679, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000546 DAOSMQ010000000
VERSION     DAOSMQ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54389_length_2058_cov_3.213679"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK829_13980"
     CDS             complement(<1..548)
                     /locus_tag="PK829_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42371.1"
                     /translation="MGFPTWTLWGMGLSAIAALLAIALSFLAQSPRMLARLHLTGSRL
                     DLRARTLTGYGLALLLLAMGFFAAGVPLGGPGAAAVEPTAEAALAEATPATAAAETLP
                     ANGSQSGAMVGLPTSSAAGEAANGVGGGLPTADLSAVAPITTTVAAGPSPAVTTAPGT
                     PTPTATATLFPTATPTPTPSPT"
     gene            684..1262
                     /gene="lepB"
                     /locus_tag="PK829_13985"
     CDS             684..1262
                     /gene="lepB"
                     /locus_tag="PK829_13985"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOU42372.1"
                     /translation="MTPPPPARPEPPPAAAFRPPTSAFVREIIETLLLTFFIFWLVNS
                     LVGRYRIDGNSMNPTLLNGEYLIISNFAYKLDAPERGDIIVFRHPNSEMNLIKRVIGL
                     PGDTVNIQNGVVSVNGVALDEPYIQAAPTYNSSWVVPEGQYFVLGDNRNNSSDSHAWG
                     FLPLDHILGKALLVYWPPSDWERIPHHEFPPS"
     gene            1515..>2058
                     /gene="rpiB"
                     /locus_tag="PK829_13990"
     CDS             1515..>2058
                     /gene="rpiB"
                     /locus_tag="PK829_13990"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOU42373.1"
                     /translation="MQPAAPTPTPTGQAGRQRVAIGADHGGYELKEALRALLAADYEV
                     IDCGTHSKDPVDYPDIAVAVARLVAAGGAWRGILIDGAGIGSCMAANKIAGIRAAMCY
                     DQATAVNSREHNNANVLTLGAGLIGPNLAQQIVRTWLNTAFAGGRHAARVAKIDALDG
                     ARPAPLAPMPPAADTKGTATK"
BASE COUNT          357 a          734 c          690 g          277 t
ORIGIN      
        1 tgcgtgggcg agggcgtcgg cgtgggcgtg gccgtgggga agagcgtcgc cgtggccgtg
       61 ggcgtgggcg tgcccggcgc ggtcgtgacc gccggcgagg ggccggccgc gacggtggtg
      121 gtgatgggcg cgacggcgga cagatcggcc gtcggcagcc cgccgccgac gccgttggcg
      181 gcctcaccgg cggcgctgct ggtgggcagg ccgaccatcg cccccgactg gctgccgttg
      241 gcgggcagcg tttcggcggc ggctgtggct ggcgtggcct cggccagggc ggcttcggcc
      301 gtcggctcaa cggcggccgc gcccggcccg cccagcggca cgccggcggc gaagaagccc
      361 atcgccagca gcagcagcgc caggccatag ccggtcagcg tccgcgcccg caggtcgagc
      421 cggctgccgg tcaggtgcag ccgcgccagc atccgcggcg attgggccag aaatgacagg
      481 gcaatggcca gcaaggccgc tatggccgac aggcccatgc cccagagagt ccaggttgga
      541 aaacccatag ctgcccacat tatagcgcgt ttagcaaagc cagcaggtgt tgcgcccggc
      601 caggaggacg gctattatct tcagggagtg cccgcgaaca ggacgcccac gagaagagac
      661 acatggcagg tatgcagccg acaatgactc ccccgccgcc ggcccggccc gaaccaccgc
      721 cggcggccgc tttccgcccc cccacgtcgg ccttcgtccg cgagatcatc gagacgctgc
      781 tgctgacctt tttcatcttc tggctcgtca acagcctcgt cggccgctat cgcatcgacg
      841 gcaatagcat gaacccgacg ctgctcaacg gcgaatatct gatcatcagc aacttcgcct
      901 acaagctcga cgccccggag cgcggcgaca tcatcgtctt ccgccacccc aacagcgaaa
      961 tgaacctcat caagcgcgtc attggcctgc ccggcgacac ggtcaacatc cagaatggcg
     1021 tcgtcagcgt caacggcgtg gcccttgatg agccgtacat ccaggccgcg ccaacctata
     1081 acagcagttg ggttgtgccc gaggggcaat acttcgtcct gggggacaat cgcaacaatt
     1141 ccagcgactc ccacgcctgg ggcttcctgc ccctcgacca catcctgggg aaggcgctac
     1201 tggtctactg gccgcccagc gattgggagc gcataccgca tcacgagttc ccgcccagct
     1261 aggtagcggc agcgccgtag cagcgccgct ttgaggccga cagggggatt atgaacgaca
     1321 accgcgcgga gatcgaagcg ctggtacgcc aggtcgtcgc ccgcacgctt ggcgcggccc
     1381 cggccgaacc ccggcgcgcg ctcattgacg aagccaccct gcgggagacg cccgccggca
     1441 cgacgctggt catcgccgcc aacgccatca tcaccccgct ggcccggcag gtggccgaag
     1501 aacgacgcat caccctgcaa ccggcggccc cgacgcccac gcccacgggc caggccggcc
     1561 gccagcgcgt ggccatcggc gcggatcatg gcggctacga gctaaaggag gcgctgcggg
     1621 cgctgctggc ggccgactac gaggtcatcg actgcggcac gcacagcaaa gaccccgtcg
     1681 attaccccga catcgccgtg gccgtggccc ggctggtggc cgcgggcggt gcctggcgcg
     1741 ggattctcat cgatggcgcg ggcattggca gttgcatggc cgccaacaag atcgctggta
     1801 tccgcgcggc aatgtgctat gatcaggcca cagccgtgaa cagtcgcgag cacaacaacg
     1861 ccaacgtcct gacgctgggc gcgggcctca tcggccccaa cctggcccaa cagattgtgc
     1921 gaacatggct gaacacggcg tttgccggcg gccggcatgc cgcccgggtg gccaagatcg
     1981 acgccctcga cggcgcgcgt ccggcccccc tcgcgcccat gccgccggcg gcggacacaa
     2041 aaggcacggc aacgaaaa
//
LOCUS       DAOSMQ010000547         2049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_54698_length_2049_cov_3.551153, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000547 DAOSMQ010000000
VERSION     DAOSMQ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54698_length_2049_cov_3.551153"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PK829_13995"
     CDS             complement(<1..530)
                     /locus_tag="PK829_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOU42374.1"
                     /translation="MSAALPVYYSPAHAQHAPEAHADVPARIDELLGAAVDFGASIRQ
                     AGDFGIEPIAAVHAPGLLALLQTAYRRFAQLKEGPRPAVPDTFAVRASAGRVTHNIWG
                     QLGYYCTDSLTPILPHTWDAAYASAQVALSAAQALFGTPLAYALTRPPGHHAYRDLYG
                     GYCYLNNAAIAARWLA"
     gene            complement(543..1013)
                     /locus_tag="PK829_14000"
     CDS             complement(543..1013)
                     /locus_tag="PK829_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU42375.1"
                     /translation="MNEQEASPTRDAVVSLREITADSLRRILRLKVSPRQEQFVADNA
                     TSIAQAHFEPKAWFRAIYADETPVGFVMLYDDTKKPEYFLWRYMIDSRYQGLGFGRQA
                     LLQVIDHVRGRPGATEMLLSYVPAEGSPEPFYAGLGFVNTGEIEDGENVMRLAF"
     gene            1151..>2049
                     /locus_tag="PK829_14005"
     CDS             1151..>2049
                     /locus_tag="PK829_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU42376.1"
                     /translation="MARRTTLAALLGLLLLAIGHTQRAEAQGNEVLQLVNAVRAEYGL
                     APVAYNAQLAAAAQGHANFIASEGIYSHYGINGSTWQDRAQAAGYPGWAGENLVGGTR
                     LTPQQGVTWWRNSAVHFSNMLNPRWTEAGVGFAVGNGQNFYVMVFGTQSESPPPRPEQ
                     PVDVPFIVAPIELAAPNPDGSIVHTVLEGHTFWAIAARYEVSIADLYLFNGLNEDSVL
                     NPGDKLVIRLADGQPPPPTPTPPTTHTVREGESLWSIAATYKTDLDTLLWLNGVTIDH
                     VVYAGDQVKVRLGEGEAPPPTPTP"
BASE COUNT          351 a          683 c          681 g          334 t
ORIGIN      
        1 tgggccagcc agcgcgcggc gatggcggcg ttgttgagat agcaatagcc gccgtagaga
       61 tcgcggtagg cgtggtggcc gggcgggcgc gtcagggcat aggccagcgg cgtgccgaag
      121 agcgcctgcg ccgccgacag ggcgacctgg gccgaggcgt aggcggcgtc ccaggtgtgg
      181 ggcaggatgg gcgtcaggct gtccgtacag tagtagccca gttgacccca gatattgtgg
      241 gtgacacggc cggccgaggc gcgtacggca aaggtgtcgg gcacggccgg ccgcggcccc
      301 tccttgagtt gggcaaagcg ccggtaagcc gtctgcaaca gagccagcag ccccggcgca
      361 tggacggcgg cgattggctc gatgccgaag tcgcccgcct ggcggatgct tgcgccaaag
      421 tccacggccg cgcccagcaa ctcgtcgatg cgcgccggca cgtcagcgtg ggcctcgggc
      481 gcgtgctgtg cgtgggcggg cgagtagtag accggcaacg cggcggacat ggccccgacc
      541 tcctagaagg ccaaccgcat gacgttctcg ccgtcttcga tctcgccggt gttgacaaag
      601 cccaggccgg cgtagaatgg ctccgggctg ccttcggcgg gcacgtagct gaggagcatc
      661 tctgtcgctc ccggccggcc gcgcacgtgg tcgatgacct gcaacagcgc ctgccggccg
      721 aagcccaggc cctggtagcg gctgtcgatc atgtagcgcc acaggaagta ctccggcttc
      781 ttggtatcgt cgtagagcat cacgaaaccg accggcgtct cgtcggcgta gatggcgcgg
      841 aaccatgcct tcggctcgaa gtgggcctga gcaatggagg tggcgttatc ggcgacaaac
      901 tgctcttgcc gcgggctgac ctttagacgc agaatgcggc gcaggctgtc ggcggtgatt
      961 tcgcgcaagc tgacaacggc gtcgcgggtg gggctggctt cttgctcgtt catggggcgg
     1021 gaccatagcg caatcgggcc gggatgcaaa acttctctcc ggcacaaacc ccatgacagg
     1081 ccaattttgg tcagccggag cttaataagg tttgccctgc ctctgcaact ggcctatagt
     1141 tgactctcgt atggcacgtc gtaccactct ggcagccctg ttggggctgc tgctcctcgc
     1201 catcggccat acgcagcgcg ccgaggccca gggcaacgaa gtcctgcaac tggtcaacgc
     1261 ggttcgcgcc gagtatggac tggctccggt ggcctataac gcccagttgg ccgccgccgc
     1321 ccaggggcac gccaacttca ttgccagcga gggcatctac agccattacg gcatcaacgg
     1381 cagcacctgg caagaccgcg cccaggcagc cggctaccct ggctgggcgg gcgagaacct
     1441 cgtcggcggc acgcgcctga cgccgcaaca gggcgtcacc tggtggcgca acagcgctgt
     1501 ccactttagc aacatgctca acccgcgctg gacagaggcg ggcgttggct tcgccgtggg
     1561 caatggccag aacttctatg tcatggtctt cggcacccag tccgagtcgc cgccgccgcg
     1621 tcccgaacaa ccggtcgatg tacctttcat cgtcgccccc atcgaactgg ccgcgcccaa
     1681 tcccgacggc tccatcgtcc acaccgtgct ggaaggccat accttctggg ccatcgccgc
     1741 ccgttacgaa gtctccatcg ctgacctcta tctgttcaac ggcctgaacg aggacagcgt
     1801 tctgaacccc ggcgataagc tggtcatccg gctggccgac ggccagccgc cgccgcccac
     1861 gccgacgccg ccaacaaccc acaccgtccg cgagggcgag tcgttgtggt ccatcgccgc
     1921 cacctacaag accgatctgg acacgctgct gtggctgaac ggcgtgacca tcgaccatgt
     1981 cgtctatgcc ggcgaccagg tcaaggtgcg gctgggcgag ggcgaagcgc cgccgccaac
     2041 gccgacgcc
//
LOCUS       DAOSMQ010000548         2048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_54723_length_2048_cov_5.130958, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000548 DAOSMQ010000000
VERSION     DAOSMQ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54723_length_2048_cov_5.130958"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..695
                     /locus_tag="PK829_14010"
     CDS             117..695
                     /locus_tag="PK829_14010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU42377.1"
                     /translation="MTLADEATLIEQARADPAAFGILYDRYVGRIYAYALRQTADVAA
                     AEDITATTFEKALRHIRRFRRPEAGLAPWLYRIAHNEIIQRARRERFTVAWGEEDLPE
                     SGPRHSGDGRPPEATLLAQERRHDLLAALRRLNDADQDVLTLRLLEGLPAADVAVVLG
                     CSRDNVYVRLHRALGRLRQQLEQLAATEAKPS"
     gene            692..1909
                     /locus_tag="PK829_14015"
     CDS             692..1909
                     /locus_tag="PK829_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42378.1"
                     /translation="MNANRNDKEWAARLEAGREDDPLLELAAQLKRAGEETQATPTVA
                     FQRQLRRDLLNQYQQPAARPWHRLGRWAASAVAVVVLAAAVGLTWFSISSSGRPSFGG
                     AALTEPATNPPGGAASVYRLLDDSITGVITETATTIDPDGQTVTKLLTPGVNVEVTTH
                     WSLPPDAGPVSAFVHLLDSEGHVVAQADGPVEAAEDAANPLPVSTLALTLPTDLSAGT
                     YELIGGLYVPTTGTRLTVNTDDGQFTAIPLGQYEVPEPAAPGQLNEAVLATLIAQRHD
                     ILHAQLLDSQQDALLVHDVSPTAGTTLGSAAPLEFAITVDYALRSAPAGILEVQIVEM
                     QSGEAGRVVGLATVDNLVQGTGTVTAVVVVNPATALSGPADLGLWLQLRTDAASPPLL
                     IEMPESFGWRYEP"
BASE COUNT          363 a          684 c          681 g          320 t
ORIGIN      
        1 gcgcgtggca ggtcgtccac gtccacaagt cgccggcgtg gcgcgcgccg cgggatggcg
       61 gctaatccgc gccgcgccgc ggcaagattt gccgccctgc ggcgttatat aggcatatga
      121 cgctcgcaga cgaagccacg ctcatcgagc aagcccgcgc cgacccggcc gcgttcggca
      181 ttctatacga ccgctacgtc ggccgcatct acgcctacgc cctgcgccag acggccgacg
      241 tggccgcggc cgaggatatc accgcgacca cgttcgaaaa ggcgctgcgc cacatccgcc
      301 gctttcgccg gccagaggcc ggcctggccc cctggctcta ccgtatcgcc cacaacgaga
      361 tcatccagcg cgctcgccgg gagcgcttca ctgtcgcctg gggggaggag gacctgccgg
      421 aaagtggccc gcgccacagc ggcgacggcc gcccgccgga ggcgacgctg ctggcccagg
      481 agcgtcgcca cgacctgctg gccgccctgc gccggctgaa cgacgccgac caggacgtgc
      541 tgacgttgcg cctgctggag gggctgccgg cggcggatgt ggccgtggtg ctgggctgct
      601 cgcgcgataa cgtctatgtg cgcctgcatc gagcattggg acggctgcga caacagcttg
      661 agcaattagc ggcgacggag gcaaagccat catgaacgcg aatcgaaatg acaaagagtg
      721 ggccgcgcgg ctggaagcgg gccgcgagga tgacccgcta ttagagttgg cggcgcaact
      781 gaagcgcgcg ggcgaggaga ctcaggccac gccgacggtg gccttccaac gtcagttgcg
      841 gcgcgacctg ctcaaccagt accaacaacc cgcggcgcgg ccgtggcaca ggttgggacg
      901 ctgggcggcg tcggccgtcg ccgtcgttgt gctggcggcg gccgtgggcc tgacctggtt
      961 ctcgatcagc agcagcggcc ggccgtcttt cggcggcgcg gcactcacgg agccggcgac
     1021 gaacccgccc ggcggcgcgg cctcggtcta ccggctcctc gacgattcga tcaccggcgt
     1081 catcactgaa accgcgacga caatcgaccc cgatggccag actgtcacga aattgctgac
     1141 cccaggagtc aacgtggaag tcactacgca ctggagtctg ccgccggacg ctggccctgt
     1201 gtcggctttc gttcacttgc tggatagcga aggccacgtc gtcgctcagg cggatgggcc
     1261 tgtggaagct gccgaagatg cggctaaccc gttgccggtg tcaaccctgg ctctgaccct
     1321 accaaccgat ctatccgccg gcacatatga actcattggc ggcctgtacg tgccgacgac
     1381 gggtacgcgg ctgacagtca acactgatga tggtcagttc acagcgatcc ctctgggcca
     1441 atatgaggtt cctgagccgg ccgcgccggg ccagctcaat gaggcggtgt tggcgacact
     1501 gatcgcgcag aggcacgaca tcttgcacgc ccagctcctt gattcgcaac aagacgcact
     1561 gctggtgcat gacgtttcgc caacggcggg cacgacactc ggcagcgcgg cgccgctgga
     1621 attcgcgatc accgttgact acgccctcag aagcgcgccg gcaggcatcc tcgaggtgca
     1681 gatcgtcgag atgcagtctg gggaagcggg ccgagttgtt ggtctggcta cggtggacaa
     1741 ccttgtccag gggactggga cggtgacggc ggtcgtcgtt gtcaacccgg cgaccgctct
     1801 gagcgggccg gccgatctgg gcctatggct gcaactgaga accgatgccg cttcgccacc
     1861 gctactcatc gagatgccag agtcattcgg ctggcgctac gaaccatagg atcgaaaaga
     1921 cggttggcgg gtcgtcaaca agaccacccg ccaaccgtca cctctatcgc cgggccgcgc
     1981 cgcttacgcc ttaggaatga tgatccacag cagcacgtag ggcagcaagc ccggtacgcc
     2041 gcccggca
//
LOCUS       DAOSMQ010000549         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_54833_length_2045_cov_3.672362, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000549 DAOSMQ010000000
VERSION     DAOSMQ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54833_length_2045_cov_3.672362"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PK829_14020"
     CDS             complement(<1..902)
                     /locus_tag="PK829_14020"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOU42379.1"
                     /translation="MFEVTFLGTSSSAPTTTRGLAGHVILHRHYRFLLDCGEGTQRQI
                     LRSGLGFKRLDTVLLTHGHLDHILGLGGLVSTLARWENLDHIDIYGSRATLARVYDLL
                     FKVVFAGHRPPIDVELHAIEPGAVICADDKFSLTTFEVSHRGPGNLGYLFAEKPHRPF
                     LAERAGELGVPFGPERARLVRGEAVVLADGRVVQPDDVLGETLHGTKYVHIGDVGRTD
                     TLIDIVRGADALVMESTYLEEEAQMAADFGHMTATRAAQLARDAGVKHLILTHLSRRY
                     SEREIRTEARATFANTHVARDLDH"
     gene            complement(895..>2045)
                     /gene="rtcA"
                     /locus_tag="PK829_14025"
     CDS             complement(895..>2045)
                     /gene="rtcA"
                     /locus_tag="PK829_14025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOU42380.1"
                     /translation="ILLFLWQSPAPAGLCVGRANRLPTGGERAIIRPMLTIDGRMGEG
                     GGQVLRTSLSLAALTGRPFRLTHVRANRAKPGLRPQHLTAVRAVARLCGAALSGDALD
                     SRTLEFRPAMPPQPGRYAFDVSDAAQGGSAGAVMLIFQAVLWPLLFAAGPSHVTLRGG
                     TAVSMSPPFHYVDRVARPVFARMGATFTLELVRWGWYPVGGGIVTAEIAPLRRLDAPE
                     FSHLPAGRVEGVAAVTNLPANIPQRMAGRATNLLAAAGHASAIEPRRENGDGPGAGIF
                     LWLPQGGFSSLGRPGLPSERVAEAAVAETLAFIDNGAMVDRHLADQLLLPLALAHGQT
                     RYTTDHLTLHTTTNADLLRQWLPVTIAITGEMGGPAEIAVTGAGFANV"
BASE COUNT          309 a          669 c          778 g          289 t
ORIGIN      
        1 tagtggtcta gatcccgcgc cacatgggta ttggcgaaag tggcgcgggc ctcggtgcga
       61 atctcccgct cgctgtagcg gcgggagagg tgggtcagga tgagatgctt cacgccggcg
      121 tcgcgggcca gttgggcggc gcgtgtggcc gtcatgtggc cgaagtcggc cgccatctgc
      181 gcctcctcct ccagataggt gctctccatc accaacgcat cggctccgcg gacgatgtcg
      241 atcagggtgt ccgtccggcc cacgtcgccg atgtggacat acttcgtgcc gtggagcgtt
      301 tcgcccagca cgtcgtcggg ctgcaccacg cggccgtcgg ccagcaccac cgcctcgccg
      361 cgcaccagcc gtgcccgctc cggcccaaag ggcacgccca actcgcccgc ccgctcggcc
      421 aggaaagggc ggtggggctt ctccgcgaac aaatagccca ggttgcccgg cccgcggtgg
      481 ctgacctcga aggtcgtcag gctgaacttg tcgtcggcgc agatgaccgc gcccggctcg
      541 atggcgtgca gttccacgtc aatgggcggc cggtggccgg caaagacgac cttgaacagc
      601 aggtcataaa cccgcgccag cgtcgcccgg ctgccgtaga tgtcgatgtg gtcgaggttc
      661 tcccagcggg ccagcgtgct gaccaggccg cccaggccga ggatgtggtc gaggtggccg
      721 tgggtcagca acacggtgtc gagccgcttg aagccgaggc cgctgcgcag gatttgccgc
      781 tgggtgccct cgccgcagtc gagcaggaag cggtagtggc ggtggaggat gacgtggccg
      841 gccagcccgc gcgtcgtggt cggcgcggac gaggacgtgc ccaggaaggt gacttcaaac
      901 attggcaaat cccgcgccgg tgacggcgat ctcggccggg ccgcccatct cgccagtgat
      961 ggcgatggtg acgggcagcc attggcgcaa caggtcggcg ttggtcgtcg tgtgcaaggt
     1021 caggtggtcg gtcgtgtagc gggtctggcc gtgggccagg gccagcggta agagcaattg
     1081 gtcggccaga tggcgatcga ccatggcccc attgtctatg aaagccagcg tttccgcaac
     1141 cgccgcctcg gcgacgcgct ccgacggcag gccgggccgg ccgaggctgc tgaagccgcc
     1201 ctgcggcagc cacaggaaga tgcccgcgcc ggggccgtcg ccgttctcgc gccgcggctc
     1261 gatggcgctg gcatgaccgg cggcggccag caggttggtc gcccgcccgg ccatgcgctg
     1321 ggggatgttg gccggcaggt tggtgacggc ggccacgccc tcgacgcgcc cggcgggcag
     1381 gtggctgaac tctggcgcgt ccagccgccg gaggggggcg atctcggccg tgacgatgcc
     1441 gccgccgacg ggataccagc cccagcgcac gagttccagc gtgaacgtcg cccccatgcg
     1501 cgcgaagacg ggccgggcga cgcggtcgac gtagtggaac ggcggactca tggacacggc
     1561 cgtgccgccg cgcagggtga cgtgtgatgg cccggcggcg aagagcagcg gccagaggac
     1621 ggcctggaag atgagcatca ccgcgccggc cgaaccgccc tgggccgcgt cgctcacgtc
     1681 gaaggcgtag cggcccggct gtgggggcat ggccgggcgg aactccagcg tgcgcgaatc
     1741 gagcgcgtcg ccgctgaggg ccgcgccgca gaggcgagcc acggcgcgga cggcggtcag
     1801 gtgttgcggc cgcaagccgg gcttagcccg gttggcgcgc acgtgggtca gccggaaggg
     1861 ccggccggtc agcgcggcga ggctaagcga ggtgcgcaga acctggccgc cgccttcgcc
     1921 catgcggccg tcgatggtga gcatagggcg gatgatcgct cgttcgccgc cggtgggcaa
     1981 gcggttggcg cggccaacac aaagccccgc cggagcgggg ctttgccata gaaacaggag
     2041 aatga
//
LOCUS       DAOSMQ010000550         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_54839_length_2045_cov_3.111055, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000550 DAOSMQ010000000
VERSION     DAOSMQ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54839_length_2045_cov_3.111055"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK829_14030"
     CDS             complement(<1..188)
                     /locus_tag="PK829_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOU42381.1"
                     /translation="MATTYYVHKGVGSDSNNGLSWANCWSSLTKAQAVAGANDTIYVR
                     AGATPYYERLGLSKNGQR"
     gene            complement(188..484)
                     /locus_tag="PK829_14035"
     CDS             complement(188..484)
                     /locus_tag="PK829_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42382.1"
                     /translation="MQAGYYAANPLEALVALTARVAMLERALASLRGGAGAQMENIAA
                     DTPLAALPDADSIPALYLVEEESRDIYRLVCRVVERQPRLYLQRVGRSRPTDTE"
     gene            complement(489..1607)
                     /locus_tag="PK829_14040"
     CDS             complement(489..1607)
                     /locus_tag="PK829_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42383.1"
                     /translation="MGGGGTPPPPPFVFVAPPLLPPVEYVLYVADRTGTVIGELVGAL
                     GAVTWSSDGYGMSSLALSPASAARYGSLLDFGRRAQIEFSGGLAPWGGFIDVPRETTR
                     GLVTAHLYEAGYALNWLLTGPQDLYLESEARPAAEILADLVRRSGMDIGLDVTLAGGG
                     PPVEAEFHYETLAAAAEKLRGLDAALHYYTRPRHTTGQRIDFDLVLFRDTGAAGGGAV
                     LVPGWNLADWTALEQGPIYNEVMVGIGDFLTVLQRPGDAVNRHELGIAIDPASQQRYG
                     RRQQPPIVLTEAEAGPGRAEARARAALEVASTPRGRVQGTCLNLPPGPFSDLRVGTRV
                     TVEASPSVLEASLLVLGMEFRPSAGTLSLVFAGDAGGG"
     gene            complement(1649..>2045)
                     /locus_tag="PK829_14045"
     CDS             complement(1649..>2045)
                     /locus_tag="PK829_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42384.1"
                     /translation="AASNLKKAFSGEAYSNPNHDIGDVASVAATISANIVAIGGDGAL
                     ATFLANLLTVGVAGTVAAGTNTTTVVTTTLPSAVSGFYVGKTFAALTGTNAKQGGKLV
                     TGYDGATKRLTIEALTAPMAAGDLFILVG"
BASE COUNT          316 a          845 c          586 g          298 t
ORIGIN      
        1 caccgctggc cgtttttgct caaccccagc cgctcatagt acggcgtcgc cccggcccga
       61 acgtaaatcg tatcgttcgc cccggccacc gcctgcgcct tcgtcaggct cgaccagcag
      121 ttggcccacg acagcccatt gttgctgtcg ctgccaactc ctttatgaac gtagtatgtc
      181 gtcgccatta ctctgtgtcc gtcggccggc tgcgccccac ccgctgcaaa tatagccgcg
      241 gctgccgctc cacgacccga cagaccagcc ggtagatgtc gcgcgactcc tcttcgacca
      301 gatacagcgc cgggatgcta tccgcgtccg gcagcgccgc cagcggcgtg tccgcggcaa
      361 tgttctccat ctgcgccccg gccccgccgc gcaacgacgc cagcgcccgc tccagcatgg
      421 ccacccgcgc cgtcagcgcc accagcgcct ccagcgggtt cgccgcgtaa tatcctgcct
      481 gcatcgcgct accctcctcc cgcgtcaccg gcaaagacca gcgacaacgt gcccgcgctc
      541 ggccgaaact ccatccccag caccagcagc gacgcctcca gaaccgacgg cgacgcctcg
      601 accgtcaccc gcgtgccgac ccgcaggtca ctgaatggcc ccggcggcag attcagacac
      661 gtgccctgga cacggccgcg cggcgtgctg gccacctcca gcgccgcccg cgcccgcgcc
      721 tcggcccggc ccggccccgc ctccgcctcc gtcaggacaa tcggcggctg ctgccgccgg
      781 ccgtagcgct gctggctggc cgggtcgatg gcgatgccca actcgtggcg gttcacggcg
      841 tcgcccggcc gttgcaaaac ggtcaggaag tcaccgatcc ccaccatcac ctcgttgtag
      901 atcggcccct gctccagcgc cgtccagtcg gccagattcc accccggcac cagcaccgcc
      961 ccgccgccgg ccgcgcccgt gtcgcggaac agcaccaggt cgaagtcgat gcgctgcccc
     1021 gtcgtgtgcc gcggccgggt gtagtaatgc agcgccgcgt ccagcccgcg cagcttctcc
     1081 gccgccgcgg ccaacgtctc gtagtgaaac tccgcctcca ccggcggccc gccgccggcc
     1141 aacgtcacat ccaggccaat atccatcccc gaccgcctga ccagatcggc cagaatctcc
     1201 gccgccggcc gcgcctcgct ctccaaataa aggtcttgcg gccccgtcag cagccagttc
     1261 agcgcatagc ccgcctcgta caggtgcgcc gtcaccagcc cccgcgtcgt ctcccgcggc
     1321 acgtcgatga agccgcccca cggggccagc ccgccggaaa attcaatctg cgcccggcgg
     1381 ccgaaatcca gcagcgaccc gtaacgcgcc gccgacgccg gcgacagcgc cagcgacgac
     1441 atgccatagc cgtcactgct ccacgtcacc gcccccagtg cccccacaag ctcaccgatg
     1501 accgtccccg tccggtcggc cacgtagagc acgtactcca ccggcggcag cagcggcggc
     1561 gcgacaaaga caaatggcgg cgggggcggc gtgccgccgc cgcccaccgg ccgtctgtag
     1621 ctccacagcc ctgtcgaacg ctgcgccatt agcccaccag gataaacaga tcgccggccg
     1681 ccatcggcgc ggtcagcgcc tcgatggtca gccgcttcgt cgccccgtcg tagccggtga
     1741 ccagcttgcc accctgcttg gcgttcgtgc cggtcagggc ggcgaacgtc ttgccgacgt
     1801 aaaacccgct caccgcgctc ggcagcgtcg tcgtcacaac cgtcgtcgtg ttcgtcccgg
     1861 ccgccaccgt gcccgccacg ccgacggtga gcaggttcgc caggaacgtc gccagagcgc
     1921 cgtcgccgcc aatagccacg atgttggcgc tgatcgtcgc cgccaccgaa gccacatcgc
     1981 cgatgtcgtg gttcgggttg ctgtacgctt ccccgctgaa cgccttcttc aggttgctgg
     2041 ccgcc
//
LOCUS       DAOSMQ010000551         2036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_55178_length_2036_cov_4.768299, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000551 DAOSMQ010000000
VERSION     DAOSMQ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55178_length_2036_cov_4.768299"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..469)
                     /locus_tag="PK829_14050"
     CDS             complement(170..469)
                     /locus_tag="PK829_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOU42385.1"
                     /translation="MFLGKVVGTLVSTQKEASLDGLKFLVVRRLTIDNQDERGYVVAA
                     DAVGAGLDEVVMVATGSSARQTVMTDKRPCDAVVMAIVDSWEVDGQEKYNKGQSA"
     gene            complement(606..896)
                     /locus_tag="PK829_14055"
     CDS             complement(606..896)
                     /locus_tag="PK829_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOU42386.1"
                     /translation="MTEALGMIECRSFAAMVEASDAMVKAARVTLVGYEKTGGGYVTA
                     IVRGDVAAVKAAVEAGARGAEKIGEIVAVHVIPRPHENVDQTLPLGRAPAAE"
     gene            complement(924..1562)
                     /locus_tag="PK829_14060"
     CDS             complement(924..1562)
                     /locus_tag="PK829_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42387.1"
                     /translation="MATELRSYVFLDSLQPQHAAFLGTVAGGFLPLPGDASLWIEISP
                     GIEINRITDIALKATDVRPGMQIIERLYGLLEIHSSSQASVRAAGAAILEAIGVVESD
                     RLKPTVVSSQIIRNIDPHQTQLINRTRHGHMLLAGQTLYVMEVQPAAYAALAANEAEK
                     RADLNILEVRAFGSFGRIYLGGEERHIMAGYQAAIDAIEGVSGREQAGKRSE"
     gene            complement(1565..1840)
                     /locus_tag="PK829_14065"
     CDS             complement(1565..1840)
                     /locus_tag="PK829_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42388.1"
                     /translation="MLTIQCEECGIGRCHYVATPYLLKLGKHMLVMPDSPSYVCDICG
                     NRFYDDEFIYGVHHLLRQAAEDTRRRARRRQIPHADRVAQPPARRSR"
     gene            complement(1845..>2036)
                     /locus_tag="PK829_14070"
     CDS             complement(1845..>2036)
                     /locus_tag="PK829_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HOU42389.1"
                     /translation="TGFGPGGATAQDVALMRRVVGPEIGVKASGGVRNYADTQAMIAA
                     GATRIGASAGVAIVSEARR"
BASE COUNT          312 a          664 c          692 g          368 t
ORIGIN      
        1 ggcgcgggct tgcggtcgtc ggcgcgcggc ggggcggccg ccggaaccgg cgccgccggc
       61 gctgcgccgg cgccaacctc ccgcatgacg cggtcgatga tggcctgaat ctcgcggtca
      121 ttcatgacaa tgtgctcgct ggccctgcgt caacagccgg cgcggccggc taggcgctct
      181 ggcccttgtt gtacttctcc tggccgtcca cttcccagga atcgacgatg gccatgacaa
      241 ccgcgtcgca gggacgcttg tcggtcatga ccgtctgccg ggcggaactg cccgtggcga
      301 ccatcacgac ctcgtccagg cccgcgccga cggcatcggc ggcgacgacg tagccgcgct
      361 cgtcctggtt gtcgatggtc aggcggcgga cgacgaggaa cttcaggccg tcgagcgagg
      421 cctctttctg ggtggagacg agcgtgccga ctactttgcc gagaaacata gatcatccat
      481 cgatgtggtc gcgattctcc gaatcgcggc cacggttggc ctgtgctgtg gtcggaggcg
      541 attcggagaa tcgcgccacg gttggccggt ggtcagaggc gattcggaga atcgcggcca
      601 cgggtttact cagcggccgg ggcgcggcca agcggcagcg tctggtcaac gttctcgtgc
      661 ggccgcggga tgacgtggac ggcgacgatt tcgccgatct tctcggcccc gcgcgcgccc
      721 gcttcgacgg cggccttcac cgcggccaca tcgccgcgga caatggcggt gacgtagccg
      781 ccgccggtct tctcatagcc taccagcgtg acgcgagcgg ccttgaccat cgcgtccgag
      841 gcctcgacca tggcggcaaa actgcggcac tcgatcatgc ccaatgcttc tgtcatttca
      901 ctttctcctg atggttgcgt accctactcg ctgcgtttgc cagcctgttc ccggccgctg
      961 acgccttcga tggcatcgat ggcggcctga tagccggcca taatgtggcg ttcctcgccg
     1021 cccagataga tgcggccgaa gctgccgaag gcgcgcacct ccaggatgtt gaggtcggcg
     1081 cgcttctcgg cctcattggc ggccaacgcg gcgtaggcgg ccggctgcac ctccatgaca
     1141 tacagcgttt gcccggccag gagcatgtgc ccgtggcgcg tgcggttgat gagctgtgtc
     1201 tggtgagggt cgatgttgcg gatgatctgg ctggagacga cggtgggctt caggcggtcg
     1261 gactcaacca cgccgatggc ctccaggatg gccgcgcccg cggcgcgcac gctggcctgg
     1321 ctggacgagt ggatttccag caggccgtag agccgttcga tgatctgcat gcccggccgc
     1381 acgtcggtcg ccttcagggc gatgtcggtg atgcggttga tctcgatgcc gggcgaaatc
     1441 tcgatccaca gcgaggcgtc accgggcagg ggcaagaagc ccccggcgac cgtgcccagg
     1501 aaggcggcgt gctgcggttg gagactgtcc aggaatacat agctgcgcaa ttcagtggcc
     1561 acaattagcg actcctgcgg gctggtggct gcgccacccg gtcggcgtgg ggaatctgcc
     1621 gccggcgggc gcgtcgccgg gtatcttcgg ccgcctgccg cagcaggtgg tggacgccgt
     1681 agatgaattc atcgtcatag aaccggttgc cgcagatgtc acaaacatac gacggcgaat
     1741 cgggcatgac cagcatatgc ttgccgagct tcagcaagta gggcgttgcg acgtagtgac
     1801 agcgaccaat tccgcactct tcgcactgaa tcgttaacac gtccttatct cctggcctcg
     1861 gatacgatgg ccacgccggc gctggcccca atgcgcgtgg ccccggcggc gatcatcgcc
     1921 tgggtgtcgg catagttgcg caccccaccg gatgccttga cgccaatctc cggcccgacg
     1981 acgcggcgca tgagggccac gtcctgggcc gtcgccccgc ccggcccgaa gccggt
//
LOCUS       DAOSMQ010000552         2028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_55454_length_2028_cov_4.736949, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000552 DAOSMQ010000000
VERSION     DAOSMQ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55454_length_2028_cov_4.736949"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PK829_14075"
     CDS             <1..901
                     /locus_tag="PK829_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42390.1"
                     /translation="VRPYLFARLPRVLASVEGFQDITQARQATVVDAIGAHCDYVIAA
                     HWGDVYLDDMGVGDAPGDLVAVAAHKFHKPGSDWLLAHLGRPHLGRAETEEMLHGLLA
                     AELARIPALGDADFTIKALKAEQWSARWTTASLRMYQSAAFPRLPFYDTRLIDFFTTV
                     PTAYVAGRRLQIDYLRRAAPDLARVPWQATGRDLFDDGGFSPRVLTQRVARKARAVAT
                     GRRVLERNWEVQFLGTDGRAGLHEWLLRPGRRLHELVSAQSIAELLDAFARDPWADKR
                     SYAVAQLLTLSAWLDYMAGDLDR"
     gene            898..2001
                     /locus_tag="PK829_14080"
     CDS             898..2001
                     /locus_tag="PK829_14080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU42391.1"
                     /translation="MTPRVLVLGLTWPPETFISRLLAGLTERGVGVTVAVGARPAVVD
                     PALARIDWLVLPGRGGSLANRLRRRIGPARGDNLPLDRFDVLYFPWNTAAIAYAPLLG
                     RKPAVISCRGAQINVAPHNPERAAIRDGLAATFAQAAAVHCVSAAIRDEAAAYGLDAG
                     KAVVIRPAVDADIFRPGAAPASPRAGGPLRIVTTGSVIWRKGYEYALLALRRLIDQGV
                     AAHFDIVGGGPEAQRLLYTIHDLALQDHVTWHGAQPPGEVLRRLQAADVFLLTSLSEG
                     IANAALEGMACGLPVVTSAVGGMGEAVTDGVEGFLAPARDPEATAAALARLAASPGVR
                     RDMGAAARRRVERDFRLSDQVGAFADLFRAVAA"
BASE COUNT          243 a          732 c          731 g          322 t
ORIGIN      
        1 cgtgcggccc tatctcttcg cccggctgcc gcgcgtgctg gccagcgtgg agggttttca
       61 ggacatcacc caggcccgcc aggcgaccgt cgttgatgct attggcgcgc actgtgacta
      121 cgtgatcgct gcccactggg gtgatgtcta cctcgacgac atgggcgtgg gcgacgcgcc
      181 cggcgatctc gtcgccgtgg ccgcgcacaa gttccacaag ccgggcagcg actggctgtt
      241 ggcgcacctc ggccgccctc acctcggccg ggcggagacg gaggagatgc tccacggcct
      301 actggcggcc gagctggcgc gcatcccggc cctgggcgac gccgatttca cgatcaaggc
      361 cctcaaggcg gagcagtgga gcgcccgctg gacgacggcc tcgctgcgga tgtaccagtc
      421 ggccgccttc ccgcgcctgc ccttctacga cacgcggttg atcgatttct tcaccaccgt
      481 gccgacggcc tacgtcgccg ggcggcgctt gcagatcgac tatctgcgtc gcgccgcgcc
      541 cgatctggcc cgcgtgccct ggcaggccac cgggcgcgac ctgttcgacg acggcggctt
      601 cagcccgcgc gtgctgacac agcgggtcgc ccgcaaggcc cgggccgtgg ccaccggccg
      661 gcgggtgctg gagcgcaact gggaggtgca atttctaggc acggatggcc gcgccgggct
      721 gcacgagtgg ctgctgcggc ccggccggcg actccatgag ttggtgtccg cccagtccat
      781 cgccgagttg ctggatgcct tcgcccgcga cccgtgggcg gacaagcgta gctatgccgt
      841 ggcccaactg ctcacgctgt cggcctggct cgactatatg gccggagacc tcgaccgatg
      901 accccgcgcg ttcttgtcct gggcttgacc tggccgccgg agacgttcat cagccgtctg
      961 ctggccgggc tgaccgagcg cggcgtgggc gtgaccgtgg ccgtgggcgc gcggccggcc
     1021 gtggttgacc cggcgctggc gcgcatcgac tggctggtat tgccgggccg gggcgggtca
     1081 ttggcgaatc gcttgcggcg gcgcatcggg ccggcgcggg gcgacaatct gccgctcgac
     1141 cgcttcgatg tcctctactt cccctggaac acggcggcca tcgcctacgc gccgctgctg
     1201 gggcgcaagc cggcggtcat cagctgccgc ggcgcgcaga tcaacgtcgc cccgcacaac
     1261 ccggagcgcg ccgccatccg cgacgggctg gcggcgacct tcgcccaagc cgcggccgtc
     1321 cactgcgtct cggccgccat ccgcgacgag gccgccgcct atggcctgga cgcgggcaag
     1381 gcggtcgtca ttcgcccggc ggtcgatgcc gatatcttcc ggccgggggc cgcgccggcg
     1441 tcgccccgcg ccggcgggcc gctgcgcatt gtcaccaccg gtagtgtcat ctggcgcaag
     1501 gggtatgagt acgccctgct ggccctgcgc cggctgattg accagggcgt ggcggcgcac
     1561 ttcgacatcg ttggcggcgg cccggaggcc cagcggctgc tctacaccat ccacgacctg
     1621 gccctacaag accacgtcac ctggcacggc gcacagccgc cgggcgaagt cctgcgccgt
     1681 ttgcaggccg ccgacgtgtt cctgctgacc agcctgagcg agggcatcgc caatgccgcc
     1741 ctggagggga tggcctgcgg cctgccggtc gttacttcgg ccgttggcgg catgggcgag
     1801 gccgtgaccg atggcgtgga gggctttctg gctccggccc gcgacccgga ggcgaccgcc
     1861 gcggcgctgg cccgtctggc cgcctcgcca ggagtgcgcc gggacatggg cgcggcggcg
     1921 cgccgccgcg tcgagcgcga cttccggctg agcgatcagg ttggggcctt cgccgacctc
     1981 ttccgggcgg tggcggcatg agactggtgc tggtcgtgcc ccggtttc
//
LOCUS       DAOSMQ010000553         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_55502_length_2027_cov_3.877282, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000553 DAOSMQ010000000
VERSION     DAOSMQ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55502_length_2027_cov_3.877282"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PK829_14085"
     CDS             <1..1061
                     /locus_tag="PK829_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU42392.1"
                     /translation="GIAEALQQLTVPTVVLTLGQRGSCARTPGGMLFQTAFPVDPVDT
                     TAAGDTFILAGVLLSNLCFVVALVLVYRLAGQTRPWSGEAGAAAGNDANGMGAAAGDA
                     AWPQAPVAPARRNHAGYLAALYLCIAPASFVFSSYLTESLFLLLTVGAFYAAERRGWL
                     AAGLLAGLAALTRSLGVFVALPLAVLWWQQRRRGEARLWQLLPLLLAPLGLALFMLIL
                     DRGVGDPLAFMHQGERWGMARAWPWTAFLSDSAARALFDPIDRGLTLLFTGLALLAFR
                     LGPAYGLWALIGTVGALALKGNPTSMLRYTVVVFPAFLVLAEIGRRHPRVHDAVLVSS
                     TVLLTLLMALSAENFWVT"
     gene            1156..1845
                     /locus_tag="PK829_14090"
     CDS             1156..1845
                     /locus_tag="PK829_14090"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HOU42393.1"
                     /translation="MNQFTPPLYFEYFALLLWAISGAIVGWRKGYDVVGVFVIAFVSS
                     FGGGLLRDGLFLQRLPVVLTDSNYIFILLIAVAGVTVVGRRLNRDKMLPKIVSIIDAI
                     GVPMFVVIGAELARSRVLELPAIVLVATISGIGGGLMRDLMAGDTPELLRPGQYNTLL
                     VVLAAVLYLLVTQYTPLTRIQTAWLTIVLFFAARLLTIRFNWRTRPLHDFHVSEVVGG
                     VAEWIPWGRHK"
BASE COUNT          243 a          727 c          663 g          394 t
ORIGIN      
        1 gcggcattgc cgaggctcta caacagctga ccgtgcccac tgtggtgctt acgctaggcc
       61 aacgcggcag ctgcgcccgc acaccgggcg gcatgctgtt tcagaccgcc ttcccggtgg
      121 atcctgtaga caccacggcc gccggcgata cctttatcct ggccggcgtg ctgctatcga
      181 acctgtgctt cgtcgtcgct ctggtgctgg tctaccggct ggcgggacag acgcgaccgt
      241 ggtcgggcga ggccggcgcg gcggccggca acgacgcgaa cgggatgggc gcggcggccg
      301 gcgacgcggc gtggccgcaa gcccccgtgg ccccggcgcg gcgaaatcac gccggctatc
      361 tggcggcgct ctacctgtgc attgccccgg cctcgttcgt cttctccagc tatctgactg
      421 agtctctctt tctgctgctg acggtgggcg cgttctatgc cgccgagcgg cggggctggc
      481 tggcggcggg gctgctggcc gggctggcgg cgctgacgcg atcgctgggg gtgttcgtgg
      541 ctctgccact ggcggtgttg tggtggcagc agcgacggcg cggcgaggcg cggctgtggc
      601 agctcctgcc cctactgctg gcgccgctgg ggctggcgct gttcatgctc atcctcgacc
      661 ggggcgtggg cgatcccctg gccttcatgc accagggcga gcgctggggc atggcccgcg
      721 cctggccgtg gaccgccttc ctctccgaca gcgccgcccg tgccctgttc gatccgatcg
      781 atcggggact gaccctgctg ttcaccgggc tggcgttgct cgccttccgc ctcggcccgg
      841 cctacgggct gtgggcgctc atcggcacgg tgggggcgct ggcgctgaag ggcaacccca
      901 cgtcgatgct gcgctacacg gtggtcgtct tcccggcttt ccttgtcctg gccgagatcg
      961 gccgccgcca cccccgcgtg catgacgccg tgctcgtttc ctccacggtg ctgttgacgc
     1021 tgctcatggc gctgtcggcc gaaaacttct gggtgacctg accggggcgg ccgctcgatt
     1081 ctccccaacc tggagcgctt ttcgcttctg cgtccatttc tgccattcaa cgggtatact
     1141 tggccgcgag gcactatgaa ccaattcact ccccccctct acttcgagta cttcgccctg
     1201 ctcctgtggg ccatcagcgg cgcgatcgtc ggctggcgca agggctatga tgtcgtcggc
     1261 gtgttcgtca tcgccttcgt gtcgtcgttc ggcggcgggt tgttgcgcga cggtctgttc
     1321 ctgcaacgcc tgccggtggt gctgaccgac tccaactaca tcttcatcct gctcattgcc
     1381 gtggccgggg tgacggttgt cggccgccgt ctcaaccgcg ataagatgct gcccaagatc
     1441 gtctccatca tcgacgccat cggcgtgccc atgttcgtcg tcatcggcgc ggaattggcc
     1501 cggtcgcgcg tgctggagct gccggccatc gtgctcgtgg ccaccatctc cggcatcggc
     1561 ggcggtctga tgcgcgacct gatggccggc gacacgcccg aactgctgcg gccgggccag
     1621 tacaacaccc tgctggtcgt cctggcggcc gtgctctacc tccttgtgac gcaatacacg
     1681 cccctgacgc gcatccagac ggcctggctg accatcgtcc tgttcttcgc cgcccgcctg
     1741 ctgaccatcc gcttcaactg gcgcacgcgc ccgctgcacg atttccacgt cagcgaggtc
     1801 gtcggcggcg tggccgagtg gatcccgtgg ggacggcata agtagagacg ggtcggcgac
     1861 ccgtctcaac gccggcgacc cgtctcaacg ctggcgaccc gtcctaacgc cggcgacccg
     1921 tctcaacgcc ggcgacccat ctctccgccg gcgacccgtc tcaacgccgg cgacccgtct
     1981 caacgccggc gacccgtctc aacgccggcg acccatctca acgccgg
//
LOCUS       DAOSMQ010000554         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_55658_length_2023_cov_5.083333, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000554 DAOSMQ010000000
VERSION     DAOSMQ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55658_length_2023_cov_5.083333"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PK829_14095"
     CDS             <1..237
                     /locus_tag="PK829_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42394.1"
                     /translation="GEGFSGLPANFAWASGTSVQEQAQWLREARDLSMTGGKVQMMIV
                     FNVDFTNFDPSGDPQAGYAMVRPDGGCPACTTLK"
     gene            411..845
                     /locus_tag="PK829_14100"
     CDS             411..845
                     /locus_tag="PK829_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU42395.1"
                     /translation="MKVRNILATKGGRVITIAPDKTVREAVALLVHHRIGAVVVADEG
                     GHLIGILSERDVMRTAAGDEGVFGRLVADVMTREVVIGMPQDDVLAVAHTMLEKRFRH
                     LPIVDDGRLIGIISIGDVLKTQRDAYQGEIDTLETRIIAEGE"
     gene            852..>2023
                     /locus_tag="PK829_14105"
     CDS             852..>2023
                     /locus_tag="PK829_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU42396.1"
                     /translation="MFPLGSAAQPLRVAIIGAGPAGFYAAERLFKESELVIEVDLYDR
                     LPTPFGLVRNGVAPDHQKIKSVTAAFDRIAANPRFRFFGNVELGRDVTVDDLKDYYHQ
                     ILYSTGAQTDRPLGIPGDDLIGSHPATEFVAWYNGHPDYRDCEFDLSQERVAVVGVGN
                     VAIDVARILCRTPDELVKTDIADYALEQLRESRVREVHILGRRGPAQAAFTAPEAKEL
                     GELADCDTIVLPEEAALDPLSEASLGGADRAELRKVEIIQELSRRQPTGKAKRLILRF
                     LVSPVELIGDENGRVRQMRLVRNVLYATDAGSLRPKATDHFETLDVGLVFRSIGYRGV
                     PLPGVPFHDSWGVIPNAGGRVLAPDTHEPLVGQYTAGWIKRGPTGVIGTNKPDAAET"
BASE COUNT          353 a          672 c          644 g          354 t
ORIGIN      
        1 ggcgagggct tcagcggcct gccggctaac ttcgcctggg cctctggtac gtcggtgcag
       61 gagcaggccc aatggctgcg cgaggcgcgt gacctgtcca tgaccggtgg caaggtgcag
      121 atgatgattg tcttcaacgt cgacttcacc aacttcgacc cgtccggcga cccacaggcg
      181 ggctatgcca tggtccggcc ggacggcggc tgcccggcct gtactactct taagtagggg
      241 ctagggagta ggggctaggg gctaggaaga gtgctcttcc cagcccctag cccctactcc
      301 ctaatccctg gcccctatta gacggcgcaa aggctacccc tttgcgcctt tttgtgttac
      361 atgttagcca acgcctggcg ctaagaccac ttcgagagga ataagtccct atgaaagtac
      421 gcaacatcct ggccacgaaa ggcgggcgag tcatcaccat cgcgccggac aagaccgtcc
      481 gcgaggccgt ggccctgctc gtccaccatc gcatcggcgc ggtcgtcgtc gccgacgagg
      541 gcggtcacct catcggcatc ctgagcgagc gggatgtcat gcgcacggcg gccggcgacg
      601 agggcgtctt cggccggctg gtggccgacg tgatgacccg tgaggtcgtc atcggcatgc
      661 cccaggacga cgtgctggcc gtggcccaca ccatgcttga aaagcgcttc cgccatctgc
      721 ccatcgttga tgacggccgg ctgatcggca tcatctcgat tggcgacgtg ctgaagaccc
      781 agcgcgacgc ctatcagggc gagatcgata cactggaaac gcgcattatc gctgaaggag
      841 agtaagtccc catgttccca ctaggatcgg ccgcgcaacc tctgcgcgtc gccattatcg
      901 gcgccgggcc ggccgggttc tatgccgccg agcgcctctt caaggaaagt gaactggtca
      961 tcgaggtcga tctgtacgac cggctgccga cgccgttcgg gctggtgcgc aacggcgtgg
     1021 cccccgacca ccagaagatc aagtccgtca ccgccgcgtt cgaccgcatt gccgccaacc
     1081 cgcgcttccg cttcttcggc aacgttgagc tgggccgcga tgttaccgtc gatgatctca
     1141 aagactacta ccatcagatt ctctactcga ccggggcgca gaccgaccgg ccgctgggca
     1201 tccccggcga cgacctcatt ggcagccacc cggcgacgga gtttgtagcc tggtacaacg
     1261 gccaccccga ctaccgcgac tgcgaattcg acctgagcca ggagcgcgtc gccgttgtcg
     1321 gcgtgggcaa cgtggccatt gacgtggccc gtatcctctg ccgcacgccg gacgaactgg
     1381 tgaagaccga cattgccgac tatgccctgg agcagctacg cgagagccgc gtgcgcgaag
     1441 tccatatcct cggccgccgc ggcccggccc aggcggcttt caccgcgccg gaggcgaagg
     1501 aactgggcga gttggccgat tgcgacacca ttgtcctgcc ggaggaggcc gctctagacc
     1561 cgctcagcga ggcctcgctc gggggagccg accgggctga actgcgcaag gtggagatca
     1621 tccaggagtt gtcgcgccgc cagccaactg gcaaggccaa gcggctgatt ctgcgtttcc
     1681 tcgtgtcgcc ggtggagctg atcggcgacg agaacggccg tgtcaggcag atgcgcctgg
     1741 tgcgcaatgt cctctatgcc acggatgccg gctcgctgcg gccgaaggcg actgaccact
     1801 tcgagacgct ggatgtgggc ctcgtctttc gctccatcgg ctaccgcggc gtacccctgc
     1861 cgggcgtgcc tttccatgat agctgggggg tcattcccaa cgccggcggg cgcgtgctcg
     1921 ccccggatac tcatgaacct ctggtgggcc agtacaccgc cggatggatc aagcgcggcc
     1981 cgactggcgt catcggcacc aataagcctg acgccgccga gac
//
LOCUS       DAOSMQ010000555         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_55767_length_2021_cov_5.402848, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000555 DAOSMQ010000000
VERSION     DAOSMQ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55767_length_2021_cov_5.402848"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1575
                     /locus_tag="PK829_14110"
     CDS             40..1575
                     /locus_tag="PK829_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42397.1"
                     /translation="MAQTNTYDPAATDRDERRWDRWGRLTLVIGVVLLVWPVLSTLAT
                     YRYPTDGWSSTAVENFDIGGPQRMTEPLTTQATPLQPNDVVIALDGRPLLAKTPPPLP
                     PDLAEGQVIRYTLERAGQTVEVDVTLIRLSPSALLLDQLQLFRSNPGNSLFVFAMLII
                     ATTVFGLRPGSLAARYLFLFAAFDFGIGLQTSSDLYAFTYPSWLSFLHGLYGFGWVYI
                     FLPCIALLALVFPVRKWPARRFPRLLPLVVSGLPLAAAVVANVLIWFGGHLSAHRILS
                     ALTFYVAGWALLVAPVALIHNLLTIHEPLARAQMRWLAFGLGFGFIVPLVTLIISVEL
                     WPVSDPRREVILVAANLALLIFPICLAVGILRYRLFDIDVIIRRTTSYAIITGLLALV
                     YFGSIVVLQGLLSPFMSDATPAVVLSTLLIAALFLPVRRRVQDVIDRRFNRTRYDAEK
                     TLAAFAATARDETDLDALLSELVRVIQETMEPEHVSVWLRPAQFPRPKGEGSEFPRPA
                     GEG"
     gene            1648..>2021
                     /locus_tag="PK829_14115"
     CDS             1648..>2021
                     /locus_tag="PK829_14115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOU42398.1"
                     /translation="MKYGFVLPSGDARAAAEMARAAEEAGWDGFFVWEPVWGVDAWVA
                     LTAAAMVTQRIRLGTMLSPLSRMRPWKLASETATLDNLSGGRVILAVGLGAPETGFAE
                     FGEATDRKTRAELMDEGLDILTG"
BASE COUNT          285 a          679 c          649 g          408 t
ORIGIN      
        1 cgatactaac cgcaatccaa tcagacaagg caggcggcga tggctcagac caacacctat
       61 gacccggcag cgaccgaccg cgacgaacga cgttgggatc gttggggccg gctaacgctg
      121 gtcatcggcg ttgtgcttct ggtgtggccg gtgctgagca cgttggcgac ttaccgctat
      181 cccaccgacg gctggagcag cacggccgtc gaaaacttcg acatcggcgg gccgcagcgc
      241 atgaccgagc cgctgacgac ccaggccacg ccgttgcagc ccaacgacgt ggtgatcgcc
      301 ctcgacggac ggccgctgct ggccaagacg ccgccgccgc tgccgcccga cctggccgag
      361 ggacaagtca tccgctatac cctggaacgc gccgggcaga cagtcgaggt tgacgtgacg
      421 ctgattcgcc taagcccgtc ggcgcttctg ctcgaccagc tccagctatt tcgcagcaac
      481 cccggtaaca gcctcttcgt cttcgccatg ctgatcattg ccacgaccgt ctttgggctg
      541 cgtcccggca gcctggccgc ccgctacctg ttcctgttcg cggcgttcga tttcggcatc
      601 ggcttgcaga catcctccga tctgtacgcc ttcacgtatc cttcgtggct ctcctttctg
      661 cacggccttt acggatttgg ctgggtctac attttcctgc cgtgtattgc cctgttggcg
      721 ctcgtcttcc cggtgcgcaa gtggccggcg cggcgctttc cgcggctgtt gccgttggtg
      781 gtgagcggtt tgccgctggc ggcagccgtg gtcgccaacg ttctgatctg gttcggcggt
      841 catctaagcg cccaccgtat cctgtcggcc ctgactttct atgttgcggg ttgggcgctg
      901 ctcgttgccc ctgtcgccct cattcacaac ctgctgacca tccatgaacc gctggcccgc
      961 gcccagatgc gctggctggc ctttggcctg gggtttggct tcattgtgcc cctggtcacg
     1021 ctcatcatca gcgttgagct ttggcccgtc tccgacccgc gtcgcgaggt gatattggtc
     1081 gcggccaatc tggcgctgtt gatcttcccc atctgcctgg ccgtcggcat cctgcgctac
     1141 cgtctcttcg atatcgacgt catcatccgc cgcacgacga gctatgccat catcaccggc
     1201 ctgctggcgc tggtttactt cggcagcatc gtcgtgttgc aggggctgct gtcgccgttc
     1261 atgagtgatg cgacgccggc cgtcgtcctc tcgacgttgc tgatcgctgc cctcttcctg
     1321 cccgtccgcc gccgggtgca ggacgtcatt gatcgccgct tcaatcgcac ccgctacgac
     1381 gctgagaaga cgctggccgc cttcgccgcc accgcccgcg acgagaccga cctcgacgcg
     1441 ctcctgtccg aactggtgcg cgtcatccag gagacgatgg agccggaaca cgtgtcggtc
     1501 tggctgcggc cggcgcagtt ccctcgcccg aagggagagg gatcagagtt ccctcgcccg
     1561 gcgggagagg gttagggaga gggcgctttt cccgcgtcca cgttgcagac cctcccacag
     1621 ggccggggag ggcgctatac tcgccgcatg aaatacggct ttgtcctacc aagtggcgat
     1681 gcccgcgcgg cggccgagat ggctcgcgcg gccgaggaag ccggctggga cggcttcttc
     1741 gtctgggaac cggtctgggg cgtggatgcc tgggtggcgc tgacggccgc ggcgatggtg
     1801 acgcaacgca tccggctggg gacgatgctc tcgcccctgt cgcgcatgcg gccgtggaag
     1861 ctggccagcg agacggcgac gctcgataac ctgtccggcg gccgggtgat tctggccgtg
     1921 gggctgggcg cgccggagac gggctttgcc gagtttggcg aggcgaccga ccgcaagacg
     1981 cgcgccgaat tgatggacga ggggctggac atcctcaccg g
//
LOCUS       DAOSMQ010000556         2015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_55988_length_2015_cov_5.303061, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000556 DAOSMQ010000000
VERSION     DAOSMQ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55988_length_2015_cov_5.303061"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PK829_14120"
     CDS             <1..425
                     /locus_tag="PK829_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOU42399.1"
                     /translation="SPTFPDYEAIIARAGLGRFVRWLGYVDEADKPVLYRQAEVFAFP
                     SRREGFGLPPLEAMACGTPVVVSDGGALPEIIGPAGFAIDPDDARGMAGAIIGIITQD
                     DLAADLRRQGPQQAARFTWDNTAIETLRVYSKVVRDDG"
     gene            524..1627
                     /gene="rfbG"
                     /locus_tag="PK829_14125"
     CDS             524..1627
                     /gene="rfbG"
                     /locus_tag="PK829_14125"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HOU42400.1"
                     /translation="MTFANTYTDLPVLVTGHTGFKGSWLTTWLLELGAKVIGYSLPEP
                     PTRPSNFVAAGLAERITDVRGDIRDYETLRQTIATHRPQLIFHLAAQPIVLRSVELPK
                     LTIDTNAGGTVNVLEAIRQTDSVRALVSITTDKVYENQEWLWGYRESDQLGGHDPYSA
                     GKAMAELAIAAYRSTYFAPDDYARHGVAIASTRAGNVIGGGDFADYRLVPDCMKALMD
                     GRPIGVRNPLSVRPWQHVLEPLSGYLWLGAQLLRHGPAFGEPWNFGPLEQKGVPAQAL
                     AEKLVELWGRGAWEHTRPGYAEVETGLLRLSWDKAAHRLDWRPVYTWVDALTEITGWF
                     KEFQAGADMGAVTRDHIAAYTARAVELGLAWAA"
     gene            1654..>2015
                     /locus_tag="PK829_14130"
     CDS             1654..>2015
                     /locus_tag="PK829_14130"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOU42401.1"
                     /translation="MNTRHAPRFLFALLVLSLVALILRLRLAPFDFVWDGRVPWERAT
                     LAPLTIRDVPLNILLFAPLGFALAGLLGGRGRAARPWAVAGGTVALGVLFSAALEGAQ
                     FFLPERVPSLADVIANGLG"
BASE COUNT          310 a          721 c          668 g          316 t
ORIGIN      
        1 cgtcgcccac ctttcccgac tacgaggcga tcatcgcgcg ggccggactg ggccgcttcg
       61 tgcgctggct cggctatgtg gacgaggccg acaagccggt gctctatcga caggcggagg
      121 tcttcgcctt tcccagccgc cgcgagggct ttggcctgcc gccgttggaa gcgatggcct
      181 gcggcacgcc cgtggtcgtc agtgacggcg gcgcgctgcc ggagatcatc ggcccggccg
      241 gcttcgccat cgaccccgat gacgcccgcg gcatggccgg ggccatcatc ggcatcatca
      301 cccaggacga cctcgccgcc gacctgcgcc gccaaggccc gcagcaggcg gcccggttca
      361 cctgggacaa caccgccatt gagacgttga gggtgtatag taaggtggtc agggacgatg
      421 gatgaaggat gaccgccgac cgctgactgc cgaccgctga ctacagacca ctgaccactg
      481 acctactgac cactgaccta ctgaccacta accacagatc actatgacat tcgccaacac
      541 ctacaccgac ctgcccgtgc tcgtcaccgg ccacaccggc tttaaaggct cgtggctgac
      601 cacctggctg ctcgaactgg gcgcgaaggt gatcggctat agcctgccgg agccgccaac
      661 gcggccgagt aacttcgtcg ccgccgggct ggccgagcgt atcaccgacg tgcgcggcga
      721 catccgcgac tacgaaacgc tccgccagac catcgccacc caccggccgc aactcatctt
      781 ccacctggcc gcgcagccca tcgtcctgcg ctccgtggag ctgcccaagc tgaccatcga
      841 caccaacgcc ggcggcacgg tcaacgtcct ggaggccatc cgccagaccg acagcgtccg
      901 cgccctggtc agcatcacca ccgacaaggt ctacgagaat caggagtggc tgtggggcta
      961 ccgcgagtcg gatcaactcg gcggccacga cccctatagc gccggcaagg ccatggccga
     1021 actggccatc gccgcctatc gcagcaccta cttcgcgccg gacgactacg cccgccacgg
     1081 cgtggccatc gcctcgacgc gggcgggcaa cgtcatcggc ggcggcgact tcgccgacta
     1141 ccggctggtg cccgattgca tgaaggcgct gatggacggc cggcccatcg gcgtgcgcaa
     1201 tccgctgagc gtgcggccgt ggcagcacgt gctggagccg ctgagcggct atctatggct
     1261 gggcgcgcaa ctcttgcgcc acggcccggc ctttggcgag ccgtggaact tcggcccgct
     1321 ggagcagaaa ggcgtgccgg cgcaggcatt ggccgagaag ctggtggagt tgtggggccg
     1381 cggcgcgtgg gagcacaccc gccccggcta tgccgaggtc gaaactggcc tgctgcgcct
     1441 cagttgggac aaggccgccc accgcctcga ctggcggccg gtctatacct gggtcgatgc
     1501 gctgacggag atcaccggct ggttcaagga gtttcaggcc ggggccgata tgggcgcggt
     1561 gacacgcgac cacatcgccg cctacacggc ccgcgccgtg gagttggggc tggcctgggc
     1621 ggcgtaggca catggccagg cgataggata accatgaata cccgccatgc gccgcgcttc
     1681 ctcttcgccc tgctcgtcct aagcctggtc gcgctgatct tgcggctgcg cctcgcgccg
     1741 ttcgatttcg tctgggacgg ccgcgtcccg tgggagcggg ccacgctggc cccgctgacc
     1801 atccgcgacg tgccgctcaa catcctgctc ttcgcgccgc tcggcttcgc gctggcgggg
     1861 ttgctgggcg ggcgcggccg ggcggcccgc ccctgggccg tagccggcgg aactgtcgcc
     1921 ctcggtgtcc ttttctcggc ggcgctggag ggggcgcagt tcttcctgcc ggagcgcgtg
     1981 ccgtcgctgg ccgacgtcat cgccaacggg ctggg
//
LOCUS       DAOSMQ010000557         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_56125_length_2012_cov_2.704139, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000557 DAOSMQ010000000
VERSION     DAOSMQ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56125_length_2012_cov_2.704139"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1886)
                     /locus_tag="PK829_14135"
     CDS             complement(114..1886)
                     /locus_tag="PK829_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42402.1"
                     /translation="MSSRRFLPLLFLFALVGLSARGRAPAAAAPAADPPFSHLWVPYA
                     TIWRSSETHSYQYYSFADPVHHRLYVAAEPDANTTVYNTDTGAVVGHVPLSPSLRDFN
                     AGNEHLVFQSVPTLARDPRVNITYHVIDAATLAPIRAVTYACPDMGCLINDVAEGPDG
                     RLYVARYAPGAIDVVDGDSGAILYTLPFAEDGNTAVSLDTYDATLYVAGYFPDEGQSG
                     LARYDISQPTPVRLSFDPIDVGALDISPDGRYLTISNGPSRLIQQYRLEPFALLWTSP
                     FDFVANFADGGVLAINTLRLIERHELTVSLDAETGQTTYSMLWLSALMGQQQEIVRPL
                     PDGGIAVFYQNRIELRRPVDYAVVLPTVFNRACFVGSIIDDFHHSGSGWPAGQSGSVT
                     YGYTPDGYTITLGEAGQWIGLSRGDYWDNADLLSVGMIQVGDPDTRVGAVGIIYGLND
                     DWSDFYTVEAIDYNHWWRNFHFHDGQWELLQAGRYVNWAPTFPIQMEFDRQRSTNEVL
                     FKLAASDYLPIDIVPGRVGIMAASGTANVSANFLNYRFDGDNCVENNYGPSLAGAAPT
                     GVAPTDVAPASIGHLRAAQWPQGE"
BASE COUNT          370 a          568 c          740 g          334 t
ORIGIN      
        1 caggatggtc atggggcgga gtatgcccca aaggggagcg gggggacagg ataggcaggg
       61 gaggcgggcg cgcattctca gatgcgcgcc cggctgccgg cggttccggt ctatcattcg
      121 ccttgcggcc actgcgccgc gcgcaggtgg ccgatagatg ccggggccac gtccgtcggg
      181 gccacgcctg tcggggccgc gccggcgagg ctcggaccgt aattattctc cacgcagtta
      241 tcgccgtcga agcggtaatt caggaagttg gcggagacgt tggccgtgcc cgaggcggcc
      301 ataatcccca cgcggccggg tacgatgtcg atggggagat aatcggaggc ggccagtttg
      361 aacagaacct cgttggtcga tctctgacgg tcaaattcca tttggatggg gaacgtgggg
      421 gcccaattga cgtatcttcc cgcttgcagc agctcccact gaccatcgtg gaagtggaaa
      481 ttgcgccacc agtggttgta gtcaatcgcc tcaacggtgt agaagtcgct ccaatcgtcg
      541 ttcagaccat agatgatgcc gaccgcgccg acgcgcgtgt cggggtcgcc gacctggatc
      601 atgcccaccg acagcaaatc ggcattgtcc cagtagtcgc cgcggctgag gcctatccac
      661 tggccagcct cgcccagagt gatggtgtag ccgtcgggag tgtagccgta ggtgacgctg
      721 ccgctctggc cggcgggcca gccgctgccg ctgtggtgga aatcatcgat gatgctgccc
      781 acgaagcagg cgcgattgaa aaccgtgggc agaaccacgg cataatcaac cgggcggcgc
      841 agttcgatcc tgttctgata gaagacagcg atgccgccat cgggcagggg ccgaacgatc
      901 tcctgctgct gccccatgag ggccgacagc cacagcatgc tgtaggtcgt ctggcccgtt
      961 tcagcgtcca gactgacggt cagctcgtgc ctctctatga ggcggagcgt gttgatggcc
     1021 agcacgccgc cgtcggcaaa gttggccacg aagtcgaagg ggctggtcca aagcagcgcg
     1081 aacggttcga ggcggtactg ctggatgagc ctcgatggcc cgttggagat ggtcaggtag
     1141 cggccgtccg gcgagatgtc cagggcgccg acgtcgatag ggtcgaagga gagccggacg
     1201 ggcgtgggct ggctgatgtc gtagcgggcc aggccgcttt gcccttcgtc ggggaaatag
     1261 ccggcgacgt agagggtcgc gtcgtaggtg tccagggaca cggccgtgtt gccgtcctca
     1321 gcgaagggca acgtgtacag gatggccccg ctgtcgccgt ccaccacatc gatggccccg
     1381 ggggcatagc gggcgacgta gagccggccg tccggcccct cggccacgtc gttgatcaga
     1441 caacccatat ccgggcacgc ataggtgaca gcgcggatgg gggccagggt ggcggcgtcg
     1501 atcacgtggt aagtgatgtt gacgcggggg tcgcgtgcca gggtcggcac cgactgaaag
     1561 acgaggtgct cattgccggc gttgaagtcg cgcaggctgg ggctaagcgg cacgtggccg
     1621 acgacggcgc cggtgtcggt gttgtagacc gtggtgttgg cgtccggctc ggcggcgacg
     1681 tagaggcggt ggtggacggg atcggcgaag gaatagtact ggtaggaatg cgtctcgctg
     1741 ctgcgccaga tggtggcgta gggcacccag agatggctga acggcgggtc ggccgccggg
     1801 gcggcggcgg ccggagcgcg cccgcgggcc gacagaccaa cgagggcgaa caggaacaag
     1861 agcggcagga agcgacgtga ggacatggct ggctcctgaa tggcggcgca gaaattggct
     1921 gcgcaatgat acagcagttg ggcgcaacgg gcactgtgac ctgacagcaa ctgtgtcaac
     1981 ggctgataga aataacagcc ccggccatag gc
//
LOCUS       DAOSMQ010000558         2011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_56142_length_2011_cov_4.180470, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000558 DAOSMQ010000000
VERSION     DAOSMQ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56142_length_2011_cov_4.180470"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PK829_14140"
     CDS             <1..520
                     /locus_tag="PK829_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42403.1"
                     /translation="VDAARPLGEGNSVFLSLSDAADRGRAPAGMRAMTLSTHTDVAAW
                     WALRPGPDESAYLARREAYAERLIAAAERAVPGLRAAIRLQLPGTPVTFQVYTSRPLG
                     MVGGFPQTSIFAARGPQTGLDNVWLVGDSVFPGQSTAGVTLGAMRVAAAITSGQAIRE
                     NRATIHAGEGCD"
     gene            606..1496
                     /locus_tag="PK829_14145"
     CDS             606..1496
                     /locus_tag="PK829_14145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU42404.1"
                     /translation="MIEPPQSRPTPAAPISTPLLFAGLLIVDSLHFVFARALLPYLPP
                     EMSAFLLLGIAAIEVGTFQAARGGIRVGVLWRHWRFFAAIGLMIAASTAINYTAVAFI
                     DPGTASLLGKTSVLFTLALGALWLRERLRPVELLGAALAIAGAFVIGFQPGAPLRLGS
                     VLVLVSTFLYALHTAVVKRHGEGIDFGSFFLWRIATTAAFLLAFATARGALVWPGRTA
                     WLLATVAATVDVVISRALYYLSLRRMPMSIHTIILTLSPVVTILWALALFGESPSVLS
                     LVGGALVVAGVAVVMLRPRR"
     gene            complement(1510..>2011)
                     /locus_tag="PK829_14150"
     CDS             complement(1510..>2011)
                     /locus_tag="PK829_14150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU42405.1"
                     /translation="NNAAIYEDHPLAEVDYDTWTASWRRVIDTNVIGAANASYCAARY
                     MIRQGGGRIVNVSSRGAFRGEPTGPAYGASKAALNAMSQSLAKHLAPYNISVGVVAPG
                     FVETDMARDALTGQSGVDIRAQSPFNRVARPAEVAYAVLFLASERAEFMTGAIIDVNG
                     ASYLRT"
BASE COUNT          266 a          675 c          684 g          386 t
ORIGIN      
        1 cgttgatgcg gctcgcccgt tgggcgaggg caactccgtc ttcctgtcgc tgtcggacgc
       61 ggcggacagg gggcgcgccc cggcggggat gcgggcgatg acgctgtcaa cgcatacgga
      121 tgtggccgca tggtgggcgc tgcggccggg gccggacgag agcgcctatc tggcccggcg
      181 cgaagcctat gccgaacgcc tgatcgccgc cgcggagcga gccgttcccg gcctccgcgc
      241 agccatccgc ttgcagttgc cgggcacacc ggtgactttt caggtctaca ccagccggcc
      301 gttgggcatg gtcggcggct tcccgcagac gtcgatcttc gccgcccgtg ggccgcagac
      361 gggcttggac aacgtctggc tggtgggcga ttctgtcttt cccggccagt cgacagccgg
      421 cgtcacgtta ggggccatgc gcgtcgccgc cgctatcacg tctggtcagg cgattcggga
      481 gaatcgcgcc acgatacacg ccggagaagg atgcgattga cggcccatcc gcgcggagct
      541 acactctcgg ccgcgaagcg tctgcccatg gatcgacgcc ggattgacta cgctcgcgac
      601 cacctatgat cgaacctccc caatcgcgcc cgactccggc cgcgcccatc agtacgccac
      661 tgctctttgc cggcctactc atcgttgaca gcctgcactt cgtcttcgcg cgggcattgc
      721 tgccctactt gccaccggag atgtcggcct ttctgctgct gggcatcgcc gccatcgagg
      781 tgggaacgtt ccaggcggcc cgcggtggca ttcgtgtggg cgtgctgtgg cggcactggc
      841 gcttcttcgc cgctatcggc ctgatgatcg ccgcctcgac agccatcaat tacacagctg
      901 tggccttcat cgaccccggc acggcctcgt tgctgggcaa gacctcagtg ctgttcacgc
      961 tggccctcgg cgcgctgtgg ttgcgggagc ggctgcggcc ggtcgaactg cttggcgcgg
     1021 ccctggccat tgccggcgcg ttcgtcattg gcttccagcc gggagcgcca ttgcgcctcg
     1081 gctccgtgct ggtgctggtt tcgaccttcc tctacgccct gcacacggcc gttgtcaagc
     1141 ggcatggcga gggcattgac tttggcagct tcttcctgtg gcgcatcgcc acgacggccg
     1201 ccttcctctt ggcctttgcc acggcccggg gcgcgttggt ctggcccggc cgcacggcct
     1261 ggctgttggc caccgtcgcc gcaacggtcg atgtcgtcat cagccgggcg ctctactacc
     1321 tctctctacg gcggatgccc atgagcatcc ataccatcat cttgactctc agtccggtgg
     1381 taaccatcct gtgggcgtta gcgctatttg gcgaaagccc gtcggtgttg agcctggttg
     1441 gtggggcgct ggtagtggcc ggcgtagccg tggtcatgct ccggccgcgg cgctagggtt
     1501 ccgcgggcct cacgtccgca gatacgacgc gccgttgacg tcgatgatcg ccccggtcat
     1561 gaactcggcc cgctccgagg ccaggaaaag gacggcgtag gccacctcgg ccggccgggc
     1621 gacgcgattg aagggactct gggcgcgaat gtccacgccg ctctgacccg tcagcgcgtc
     1681 gcgggccatg tcggtttcca cgaagccggg ggcgacgacg ccgacagaaa tattgtaggg
     1741 cgccaggtgt ttggccagcg attggctcat ggcgttcagc gccgccttac tggccccgta
     1801 ggccgggccg gtcggttcgc cgcggaacgc gccgcgcgag gagacgttga caatgcggcc
     1861 gccgccctgg cggatcatgt accgggcggc gcagtagctg gcgttggccg cgccgatgac
     1921 gttggtgtcg atgacgcgtc gccagctggc cgtccacgtg tcgtaatcga cctcggccag
     1981 gggatggtcc tcgtagatgg cggcattgtt g
//
LOCUS       DAOSMQ010000559         2008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_56254_length_2008_cov_3.952381, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000559 DAOSMQ010000000
VERSION     DAOSMQ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56254_length_2008_cov_3.952381"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PK829_14155"
     CDS             <1..265
                     /locus_tag="PK829_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42406.1"
                     /translation="VVPPPQATSAIAPKSSTTINRYHFVFFIFPSLIELNHVTPVGFY
                     VGLADVLGNKRFFLRLITSIAAAGASDSQVYMNAKKSISALTV"
     gene            complement(254..694)
                     /locus_tag="PK829_14160"
     CDS             complement(254..694)
                     /locus_tag="PK829_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU42407.1"
                     /translation="MTGREHLYDLIKESFLLLDFGDRTLFERYGLSVSRYYALHHIAG
                     NPGLSPSRLSQLMFCDKSNITRLLQGLEVDGYVTRHVHERDARIQRLFLTDKGLRLHR
                     EVWTVHLTFIEQRLGLLSSEDSQQLETTLDCFIRALAEARSLNG"
     gene            878..>2008
                     /locus_tag="PK829_14165"
     CDS             878..>2008
                     /locus_tag="PK829_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU42408.1"
                     /translation="MLTIIPLRPTGRRRQVWLWLALALLAAGCRGPGSPATTAPTLIV
                     PAATPAGAINEPTPLPGSIAVLLPDDALDSRWGSVDRPAFERCLTDAGAVFTLYSAGG
                     DPATQAAQAAEAIAAGARVLLLAHVSNEAGAAVLATARAEGIKVIDYDRLTTGGPGAD
                     LYIGFDPLAVGRLMGETLAPFVRALPVARRNVVVLAGPAGDDTALLVAEGLRAVVEPY
                     VSAGQWTLLADLTVADATAAQAELATLLAGDAPVGAVLATDDVLAGAAAAAVSAAGLD
                     PVRLTGRGATVPALQRILAEQQTLTIYEPAKPLIGAACQAALAVLNGDEIVSLTTRAI
                     DNGAGEVPFIRLSPIAVTADNLAETVVADGAASWAEICVGAAA"
BASE COUNT          353 a          657 c          638 g          360 t
ORIGIN      
        1 ggtcgtgccg ccgccgcaag cgacgagcgc catcgcgccc aaaagcagca cgacaatcaa
       61 cagataccac tttgtattct tcatattccc ctcacttatc gaattgaacc atgttacccc
      121 tgtgggcttc tatgttggtt tggcagacgt tttgggaaac aagcgcttct ttcttcgatt
      181 gatcacctcc atagctgccg caggagcgtc tgacagccaa gtatacatga atgcgaaaaa
      241 atctataagc gcgttaaccg tttagtgacc tcgcctctgc aagcgcccgg ataaaacaat
      301 cgagcgtcgt ctccagctgc tggctatcct cactggataa caggccgagc cgctgctcaa
      361 tgaacgtcag gtggaccgtc cagacctcgc gatgcaaccg cagacccttg tctgtcagga
      421 acaggcgctg aatgcgggcg tcgcgctcgt ggacgtgacg ggtcacatag ccatcgacct
      481 ccagcccctg cagcaagcgg gtgatgttgc ttttatcgca gaacatcaac tggctaaggc
      541 ggctgggcga cagaccagga ttgccggcga tgtggtggag ggcgtagtag cgcgagaccg
      601 acagaccata gcgctcaaag agcgtgcgat cgccgaaatc cagcagtaaa aaggactcct
      661 tgatcaagtc gtacagatgc tcccggccgg tcatagcttg cctactagtg tgcactgcat
      721 ggatgacaat cagttgacag cgcaacagtt accggaatta ctctaacata tggcccatag
      781 ggtgtcaacg cggcgacatg aaatatactc ataaatgtac ctctccttat cttctagcct
      841 ttacacccag cgggccgccc gccccaggag gttgacgatg ctgacgatca ttccactccg
      901 ccccaccggc cgccggcgac aggtctggct gtggttggct ctggccttgt tggccgccgg
      961 ttgtcgcggc ccaggcagcc cggcgaccac ggcgccaacg ctcatcgtgc ccgcggccac
     1021 gccggcgggg gcaattaatg aacccactcc cctccccggc agcattgccg ttttgctgcc
     1081 cgacgacgcg cttgactcgc gttgggggtc agtcgaccgg cccgccttcg agcgctgcct
     1141 gaccgacgcc ggcgccgtct tcacgctcta cagcgccggc ggcgacccgg ctacacaggc
     1201 ggcacaggcg gccgaggcca tcgccgcggg ggccagggtg ctgctgctgg cccacgtcag
     1261 caacgaggcc ggcgcggctg tgctggccac ggcgcgcgct gagggcatca aggtgatcga
     1321 ctatgaccgt ttgaccaccg gcgggccggg agccgatctc tacatcggct tcgatcccct
     1381 ggctgtgggc cgtctcatgg gcgagacgtt ggccccgttc gtgcgtgccc tgcccgtggc
     1441 ccgccgcaat gtcgtcgtac tggccgggcc ggccggcgac gacacggcgt tgctggtagc
     1501 cgaggggctg cgcgcggtgg tggagccgta tgtcagcgcg gggcaatgga cacttctggc
     1561 cgacctgacc gtggccgacg cgaccgcggc ccaggcggag ttggccacgc tcttggccgg
     1621 ggatgcgccg gtgggggccg tgttggccac ggacgacgtt ctggccggcg ccgccgccgc
     1681 tgctgttagc gccgcgggcc tcgaccccgt gcgcctgacc ggccgcggcg caaccgtgcc
     1741 cgcgctgcaa cgtatcctgg ccgagcagca gacgctgact atctacgagc cggccaagcc
     1801 cttgattggc gcggcctgcc aggcagccct ggccgtgctg aacggcgacg agatcgtctc
     1861 gctgaccacg cgggcgatcg ataatggcgc gggcgaggtg ccgttcatcc gcctgtcgcc
     1921 catcgccgtg acggccgaca acctggccga gacggtcgtg gccgacggcg cggcgagctg
     1981 ggcagagatt tgcgttggcg cggcggcc
//
LOCUS       DAOSMQ010000560         2003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_56421_length_2003_cov_4.187885, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000560 DAOSMQ010000000
VERSION     DAOSMQ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56421_length_2003_cov_4.187885"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="PK829_14170"
     CDS             complement(<1..1393)
                     /locus_tag="PK829_14170"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HOU42409.1"
                     /translation="MLALWLLALSAACAPLPAAVAPAPAVTPVAVPITLVADGQSRAL
                     LTTARSVGEALAQAGIETSPADEIDPPVATPLIVDAAATPLTITIVRVTETTEVIPEA
                     IPFGRQIVRSAELSPGDPPRLLQAGRPGLREVTVRIVYHDGLEVERWPTATTVVEPAQ
                     NEVVMIGVIAASDRMTFAGTLAYIDDGRAILLEGATDAPRQLAIEGALDGRVFQLSPA
                     GDYLLYTAAAAKDADTFRNELWVLPTAAGGIAQPLNIENVLWAGWDPTATPPRIAYTT
                     ARSTTLPPGWEASNDLWLLEVTLDANQPAPVRLVESYPAAYAWWGGVYNWSNDGRLAY
                     AFADEVGVLTMPTAEEAAALDPTTAAPARAVLHEFAEFNTGGDWAWLPALSWSADGRL
                     LAFTDHAAATGAARERFDLYLADVVAGNQATLVEDIGIWAAAQWSPAATLPAQQLATL
                     RAIDPEAGQDGSYA"
     gene            complement(1419..1811)
                     /locus_tag="PK829_14175"
     CDS             complement(1419..1811)
                     /locus_tag="PK829_14175"
                     /inference="COORDINATES: protein motif:HMM:NF021536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HOU42410.1"
                     /translation="MTAERFYTDVMQQGQLRTLGHAQRLTGAVLHTLGFNLSGGLKRA
                     LARVLPPVLAHELTRGWRLLHFRNTRISLEDFAADVGLHSGNTDPLYNRMATAAVFGQ
                     IKRLIDNDLSRKVAKDLSPEVRELWNSA"
BASE COUNT          319 a          638 c          729 g          317 t
ORIGIN      
        1 gggcatagct gccatcctgg cccgcctcgg ggtcgatggc ccgcagcgtg gccaactgtt
       61 gcgccggcag cgtcgccgcc ggcgaccact gcgccgcggc ccagataccg atgtcctcga
      121 cgagagtggc ctggttgccg gcgaccacgt cggccaagta gaggtcgaag cgttcgcgcg
      181 ccgcgcctgt ggccgcggcg tggtcggtaa aggccagcag ccggccgtcg gccgaccaac
      241 tcagcgccgg cagccaggcc cagtcgccgc cggtgttgaa ttcagcgaac tcgtgcagca
      301 cggcgcgggc gggcgcggcc gtcgtcgggt cgagcgccgc cgcctcctcg gccgtgggca
      361 tggtcagaac gccgacctca tcggcgaagg cgtaggccag ccggccgtcg ttcgaccagt
      421 tgtagacgcc gccccaccag gcgtaggcgg ccggatagct ctcgaccagc cggacgggcg
      481 cgggctggtt ggcgtccagc gttacctcca gcagccacag gtcattgctg gcttcccaac
      541 cgggcggcaa ggtggtcgag cgggcggtgg tgtaggcgat acgcggcggc gtcgccgtgg
      601 ggtcccagcc ggcccagagg acgttctcga tgttgagggg ctgagcaatg ccgccggcgg
      661 ccgtgggcaa gacccatagc tcattgcgaa atgtgtccgc gtccttcgca gcagcggctg
      721 tatagagaag gtagtcgccg gccggcgata actggaagac acggccgtcg agcgcgccct
      781 cgatggccag ttggcgtggc gcgtcggtgg ccccctccag caggatagcc cggccgtcgt
      841 cgatgtaggc caatgtgccg gcaaaggtca tgcggtcgct ggcggcgatg acgccgatca
      901 tgacgacctc gttttgcgcc ggttcgacga cggtcgtggc cgtgggccag cgctcgacct
      961 ccaggccgtc gtggtagacg atgcgcacag tcacctcgcg caggccgggc cggccggctt
     1021 gcagcagccg cggcgggtcg ccgggtgaca gttcggcgct gcggacgatc tggcggccga
     1081 aggggatggc ttcagggatg acttcggtcg tctccgtcac ccggacgatg gtaatggtca
     1141 ggggcgtggc cgcggcgtca acgatcaggg gcgtggccac gggcgggtcg atctcgtcgg
     1201 ccggactagt ttcgatgccc gcctgcgcca gggcctcgcc gacggagcgg gctgttgtca
     1261 gcagggcgcg gctctgcccg tcggcgacaa gggtgatggg cacagcgacc ggcgtgaccg
     1321 ccggcgcggg cgcgacggca gccggcaagg gcgcgcaggc cgcgctcagt gccaggagcc
     1381 agagggccag cacggccgcg ccgccccacg tggcagacct acgcgctgtt ccaaagctcg
     1441 cgaacttcag ggctgaggtc cttggccact ttgcggctca gatcgttgtc aatgaggcgc
     1501 ttgatctgac cgaagacggc cgccgtggcc atccggttgt agagagggtc agtgttgccg
     1561 ctgtgaaggc ccacgtcggc ggcgaagtcc tccagcgaga tgcgcgtgtt gcgaaagtgg
     1621 agcaagcgcc aaccgcgcgt caattcgtgg gccagcaccg gcggcaggac gcgcgccagg
     1681 gcgcgcttga ggccgccgct gaggttgaag cccaacgtgt gcagcaccgc gccagtcagg
     1741 cgctgggcgt ggcccaacgt gcgcagctgg ccctgctgca tcacgtcagt gtagaaccgt
     1801 tccgctgtca tcgtttgatc gctcatgaaa gctgcattcc cggattgact tggccgaatt
     1861 tgtggctata cttcactgtt cggtcggaca gacagcaagc gctatcgacc gtcgattata
     1921 gccgacaaga catttagtaa ggaatataac cgcagcgggt gtggcgtaca agtacggcgt
     1981 acaagtacgg cgcacaagtc cgt
//
LOCUS       DAOSMQ010000561         2003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_56427_length_2003_cov_3.464066, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000561 DAOSMQ010000000
VERSION     DAOSMQ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56427_length_2003_cov_3.464066"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PK829_14180"
     CDS             <1..1408
                     /locus_tag="PK829_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42411.1"
                     /translation="QTAQPCSTCKPVPLLSQTALQERILAIYGPNGSGQVRPDNITSF
                     SFADLNDYLDAGTEPIPQPTAPPAATGDAATSAPGTPEPTVTLPADYRVQEALREADW
                     LVFALLSAGESAGGPDSGALNRFLAQRPDLASQSEVVVLAFDAPYFLDSTEISKLSAY
                     YAIYSHTEAFVDAAARALFLESPLLGAPPVSVEGIQYDLFTQTQPAADQVIELFFVIG
                     EQIEAPSRQEPLASAIGDTLRLQTGTILDRNGRPVPDGTLVRFILRNRVQGTVTVLGD
                     RPTTNGIAQLDYVLDASMGPGQFRITAESGDATVSQEVDIVIEDDAQLAIIVPTAAPT
                     HTPTPEPTATPTPEPTETPPPQLPTATPPRPGPTEEPGWLIGLSEARTLLAVLSGLLA
                     VGLAAVVLDRPAQPPLTQRVGRLLWGLVGGLLLYNYYVLDMPGAGTLAGLGSLAGLLL
                     VLAGGAAGLFLYRALHPG"
     gene            complement(1409..>2003)
                     /locus_tag="PK829_14185"
     CDS             complement(1409..>2003)
                     /locus_tag="PK829_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU42412.1"
                     /translation="PQPLDQVGLGVAISAVAAVANFLVARVLMRAGKRHRSAALTADA
                     HHLLTDVWTSLGVIVGVGLVGLTGVLWLDPIIGLVVAAQIIYTGLKLIRVAFNGLLDA
                     SLPADELAAVTAILERYEPEGVAYHALRTRQAGAQRFVSFHLLVPGSWSVQEGHEMLE
                     RIERDIRRKLDPVSVTSHLEPREDPVSLDDDPLFRED"
BASE COUNT          333 a          725 c          649 g          296 t
ORIGIN      
        1 ccagacggcg cagccctgtt cgacctgcaa gcctgtgccc ctgctctcgc aaacggcgct
       61 acaggaacgt atcctggcca tctatggccc gaacggcagc ggccaggtgc gccccgacaa
      121 catcaccagc ttctcattcg ccgacctgaa cgactacctg gacgcgggca cagagccaat
      181 cccccagccg accgcgcccc ccgccgcaac cggcgacgca gccacgtcgg cgccgggcac
      241 gccggagcca acggtgacgc tgccagccga ctatcgcgtc caggaggctc tgcgcgaggc
      301 cgactggctc gtgttcgccc tgctgagtgc tggggagagc gccggcgggc cggactccgg
      361 cgcgctgaac cgcttcctgg cccagcggcc cgacctggcc agccagagcg aggtcgtcgt
      421 cctggccttc gacgcgcctt actttctgga tagcaccgag atcagcaagc tgagcgccta
      481 ctacgccatc tatagccaca ctgaggcgtt tgtcgatgcc gccgcgcggg cgctcttcct
      541 ggaatcgccc ctcctcggcg cgccgccggt cagcgtggag ggcattcagt atgacctctt
      601 cacccagacg cagccggccg ccgaccaggt gatcgaactg ttcttcgtca tcggcgagca
      661 gatcgaagcg ccttcgcgcc aggagccgtt ggcctccgcc atcggcgaca cattacggct
      721 gcaaacaggg acgatcctcg accgcaatgg ccggcccgtc cccgacggca cgctggtgcg
      781 cttcatcttg cgcaaccgcg tccagggcac agtgaccgtt ctgggcgacc ggccgacgac
      841 caacggcatt gcccaactcg actacgtgct cgacgccagt atggggccgg ggcagttccg
      901 catcaccgcc gagtcgggcg acgccactgt gtcgcaggaa gtggatatcg tcattgagga
      961 cgacgcgcaa ctggccatca tcgtgccgac ggccgcgcca acgcacacac cgacgcccga
     1021 gcccactgcc acgcccaccc ccgaaccgac agaaacaccc ccaccccagt tgccgacggc
     1081 tacgccgccg cggcccggcc cgacggaaga gccgggctgg ctgatcggct tgtcggaagc
     1141 gcgcacgctg ctggccgtgc tcagcgggtt gctggctgtc gggctggcgg ccgtggttct
     1201 cgaccggccc gcgcagccgc ccctgacaca gcgggtcggc cggctgctgt gggggctggt
     1261 cggcgggctg ctgctctata actactacgt tctggatatg cccggcgcgg ggacgcttgc
     1321 cggcctgggc agcctggccg ggttgctgct ggtgttggct ggcggcgcgg cggggctgtt
     1381 cctctaccgc gccctgcacc ctggctagtc aatcctcgcg gaagagcgga tcatcatcga
     1441 gcgagacagg gtcctcgcgc ggttccaggt ggctggtgac gctgaccgga tccagcttgc
     1501 ggcggatgtc gcgctcgatc cgttccagca tctcgtgccc ctcctggacg ctccacgagc
     1561 cgggcacaag caggtggaaa gacacaaagc gttgcgcgcc cgcctgccgc gtgcgcaggg
     1621 catggtaagc gacgccctcc ggctcatacc gctccagaat agcggtgacg gcggccagct
     1681 catcggccgg cagcgaggcg tccaacagcc cattgaacgc cacgcggata agcttaaggc
     1741 cagtgtagat gatctgcgcg gccaccacca ggccgatgat cgggtcgagc cacagcacgc
     1801 cggtcaggcc caccagtccc acgccgacga tgacgcccag cgatgtccac acgtcggtca
     1861 aaaggtggtg ggcgtcggcc gtcagcgccg cggagcgatg gcgtttgccc gcgcgcatca
     1921 gcacccgggc cacgaggaag ttggccacgg cggccacggc cgaaatggcg acgcccagac
     1981 cgacctgatc taacggctgc gga
//
LOCUS       DAOSMQ010000562         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_56688_length_1996_cov_3.267903, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000562 DAOSMQ010000000
VERSION     DAOSMQ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56688_length_1996_cov_3.267903"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1890)
                     /locus_tag="PK829_14190"
     CDS             complement(163..1890)
                     /locus_tag="PK829_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOU42413.1"
                     /translation="MVPTKLDTRRPGRAAVARVWLAAAALLLFFFLAVNSLVGDSPTM
                     DEQNHIARGLAFLRTGDPRLSLEHPPLINALSALPLLTMPELRLPTDHASWERREGWY
                     EFADLFLWQYNHDVGRIVFLARLPVVFLLMGLALTGYRYARALWGAAAGLPALCLLLF
                     EPNLLAHGRYATTDMGGTLFTFLATALLWRLWQEPERWNWRRWAWAALAVGLAVGSKM
                     TALGFVPIWVVLALLPLYPVGGRGRVWPAGRRLAQLAAAGLVALVVLWAIYGFQWGNF
                     LFISPALQPLNTAAGPMPTFWAGVEQIARLGGAGRAQSFLLGRFSDSGFVAYFPVAFA
                     VKTPLVLLAAIPLAAVWLLGDARTRRRALFLFAPALLYFALTMVSALNIGYRHALPAL
                     PHLLVLVAGLAAPEATGRRLFGSAALGARRLVAATVAVVIGVALWIHPHYLSYFNLAA
                     GGPANGYRVLIDSNVDWGQDLLRLQQWMAETGTASVRLGWFGTADPGYYGLAYEPLPG
                     IGRDTFFRLWWALPFNPQQPEPGVYAISATSLWETPLRPEEKRVYAWFRAHEPSARVG
                     YSILIYDVP"
BASE COUNT          387 a          685 c          695 g          229 t
ORIGIN      
        1 atgaagaggg cgatgtcggc gtcgagaatc tcgtcctggg ccaggcccgg cggcacgttg
       61 ggcagggcca ccagccggaa ggccgcggcc agcagcagga cgagcagcgc cgccagcaac
      121 cagcgccgcg gcgacggccg ccacccggca agggaagctc gttcagggga catcgtagat
      181 gaggatggaa tagccgacgc gggccgacgg ctcatgggcg cggaaccagg catagacgcg
      241 cttttcctcc ggccgcagcg gcgtctccca caggctggtg gcgctaatgg cgtaaacgcc
      301 cggctccggc tgctgcgggt tgaagggcag cgcccaccac agccggaaga aggtatcgcg
      361 gccgatgcca ggcagcggct cgtaggccag gccgtagtag ccgggatcgg ccgtgccgaa
      421 ccagcccagg cgcacgctgg ccgtacccgt ctcggccatc cactgctgca agcgcagcag
      481 gtcttgcccc caatcgacgt tgctgtcgat gaggacgcgg tagccgttgg ccgggccgcc
      541 agcggcgagg ttgaagtagc tcaggtagtg gggatggatc cacagggcta cgccgatgac
      601 cacagccacg gtcgccgcca ctagccgccg cgcccccagc gccgcgctgc caaagagccg
      661 ccgcccggtc gcctccggcg cggccagccc ggcaacaagc accagcagat ggggcagcgc
      721 cggcagggcg tggcgatagc caatgttgag cgcgctgacc attgtcaggg cgaagtagag
      781 caacgccggc gcgaacagga acagcgcccg gcggcgcgtg cgagcgtcgc ccaacagcca
      841 gacggccgcc agaggaatgg ccgccagcaa caccagcggc gtcttgacgg caaaggccac
      901 cggaaagtag gccacgaaac cgctatccga aaagcggccc agcagaaacg actgcgcgcg
      961 cccggcgccg cccaggcgag cgatctgctc cacgccggcc cagaaggtcg gcattggccc
     1021 tgcggccgtg ttcagcggtt gcagcgcggg cgagatgaac aggaagttgc cccactggaa
     1081 gccgtagatg gcccagagaa cgaccagagc gaccagcccc gccgcggcca gttgcgccag
     1141 ccgccgcccg gccggccaga cccggccgcg cccgcccacg gggtagagcg gcagaagggc
     1201 cagcaccacc cagatgggca cgaagcccag cgcggtcatc ttgctgccga cggccagccc
     1261 gaccgccagc gctgcccagg cccagcgccg ccagttccag cgctctggct cctgccacaa
     1321 gcgccacagc agggccgtgg ccagaaaggt gaacagcgtg ccgcccatgt cggtggtggc
     1381 atagcggccg tgggccagca ggttcggctc gaacagcagc aggcacagcg ctggcagccc
     1441 ggcggccgcg ccccacagcg cccgcgcgta gcggtagcca gtcaaggcca gacccatgag
     1501 caggaagacc accggcagcc gcgccaggaa aacgatccgg ccaacgtcgt ggttgtattg
     1561 ccacaggaaa aggtcggcga actcatacca gccttcgcgc cgttcccaac tggcatggtc
     1621 ggtgggcagg cgcaactccg gcatggtcag tagcggcaaa gcactgaggg cattgatgag
     1681 cggcgggtgc tccaggctga ggcgcgggtc gccggtgcgc aggaaggcca gcccgcgggc
     1741 gatgtggttc tgctcgtcca tcgtcggact gtcgccgaca aggctattga cggccaggaa
     1801 gaagaacaac agcagcgccg ccgccgccag ccagacgcgg gcgacggccg ctcgccccgg
     1861 tcgccgcgtg tccaattttg tgggcaccat gccgtcatta tagcggcata gtgggccgtg
     1921 gacagttgtc cgccgcaccg acctgacacc ctaattttgc gcaaaaatcg gggggtatca
     1981 aaggtccacc gagcag
//
LOCUS       DAOSMQ010000563         1988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_56999_length_1988_cov_3.023280, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000563 DAOSMQ010000000
VERSION     DAOSMQ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56999_length_1988_cov_3.023280"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PK829_14195"
     CDS             <1..1004
                     /locus_tag="PK829_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42414.1"
                     /translation="FLPERVPSLADVIANGLGALLGAALFNAWAMGVGRALERVLTWR
                     NVLVGLALYALGAALLTAHLGRSVFLDNWDTSFPLVVGNEAVGKRQWSGKVHNLYFTA
                     YCDSDVVSWAGYDFDGQAPYVDTFEDFNYPALDWSEGSSTQQDGQGVLLGPGEWLTTT
                     EPFSEFSKYARLCHSIWISTTVATTDPAQRGPARIISISADAEHRNVTLGQEKDALII
                     RLRTPASGENGQKPEILVPGVFTDGRMRRIEVRYIAAMVAPMLYVWVDGVEYSLSLAP
                     GAAFFPGFVTENRWPVTLGGNPFRYDLAYLAIVVGLPAVVIGGLWAARGLSRRRGWR"
     gene            complement(1185..1946)
                     /gene="pgl"
                     /locus_tag="PK829_14200"
     CDS             complement(1185..1946)
                     /gene="pgl"
                     /locus_tag="PK829_14200"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOU42415.1"
                     /translation="MTTTNMPSTPTPDVRVVAGMAAAAGYVVAALQAALSDGAVATLV
                     LAGGTTPLPLYARLAEATDLDWERIHVFWGDERLVPPDDPGSNYAAAHEALLRHVPLP
                     EANIHRIKGELEDVAAVADYAEQLRGFAQRYDAGCPSPWPRFDIVLLGLGADGHTASL
                     FPGSPAACVVPVMAVTADYDGRPAQRVTLTPIVFNHARRVAFLVSGANKAAAVAAALG
                     PERDPERYPAQRMEPEGELVWFVDEAAAEGLGARG"
BASE COUNT          304 a          650 c          696 g          338 t
ORIGIN      
        1 tcttcctgcc ggagcgcgtg ccgtcgctgg ccgacgtcat cgccaacggg ctgggggcgc
       61 tgctgggcgc ggcgctgttc aatgcctggg cgatgggcgt cgggcgcgct ctggagcggg
      121 tcctcacctg gcgcaacgtg ctcgtcggcc tggccctcta tgccctcggc gcggcactgc
      181 tgacggccca cctgggtcgc agcgttttcc tggacaactg ggacacgtcc ttcccgctgg
      241 tggtcggcaa cgaggcggtg ggcaagcggc aatggagcgg gaaggtacac aatctgtatt
      301 tcacggccta ctgtgatagc gatgtggtca gctgggccgg gtacgatttt gacgggcaag
      361 cgccctacgt ggacactttt gaggacttca actatccagc gcttgactgg agcgaagggt
      421 cgtccactca acaggatggc cagggcgtgc tacttgggcc gggcgagtgg ctgaccacaa
      481 ctgagccatt cagcgagttc tctaagtatg cccgtctctg ccatagcatc tggatcagta
      541 cgactgtggc cacaaccgac ccggcgcagc gcggcccggc gcggatcatc tcgatctcgg
      601 ccgacgccga gcaccgcaac gtgaccctgg gccaggagaa ggacgcgctc atcatccgcc
      661 tgcgcacgcc cgccagtggc gagaacggcc aaaagccaga aatactcgtg cccggcgttt
      721 tcaccgatgg gcggatgcgt cgtatcgagg tgcgctacat cgcggcgatg gtggcgccga
      781 tgctgtatgt ctgggtggat ggcgtggagt atagtctgtc cctcgctccg ggggcggcgt
      841 tcttccccgg ttttgtgacg gagaatcgct ggccggtgac cctgggcggc aaccccttcc
      901 gctacgacct ggcctatttg gccatcgtcg tcgggctgcc ggccgtggtc atcggcgggc
      961 tgtgggcggc ccgcggcctc agccgtcggc gcggctggag ataggggcta gggagcaggt
     1021 tccaggggaa gaaggctctt ccctatcccc agggtttttc caaagtcgtc tcgcgaccat
     1081 tgacctgaca ggtgagcggc ccgtcgttct ctggaaaggc ccatgcgtgg ccgcccacct
     1141 gtcaggtcgg tgcgccgggg gtgtctttgg caaagccctg gcccctagcc cctagcccct
     1201 agcccctccg cggcggcctc gtccacgaac cacaccaact ccccctccgg ctccatacgc
     1261 tgcgcgggat aacgttccgg gtcgcgctcc gggccgaggg cggcggccac ggcggcggcc
     1321 ttgttcgcgc cgctgaccag gaaggccacc cgccgggcgt ggttgaagac gatgggcgtc
     1381 agggtgacgc gctgggcggg gcggccgtcg tagtccgcgg tcacggccat gaccggcacg
     1441 acgcaggccg ccggcgagcc ggggaacagc gacgccgtgt gcccgtccgc gcccaacccc
     1501 agcagaacga tgtcgaagcg cggccacggc gacggacagc cggcgtcgta gcgctgggcg
     1561 aagccgcgca actgctcggc gtagtcggcc accgcggcga catcctccag ttcacccttg
     1621 atgcggtgga tgttggcctc cggcaggggc acatggcgca acaacgcctc gtgggcggcg
     1681 gcgtagttgc tgccggggtc gtcgggcggc accaggcgct cgtcgcccca gaagacgtgg
     1741 atgcgctccc agtcgagatc ggtcgcctcg gccaggcggg cgtagagcgg cagcggcgtc
     1801 gtcccgccgg ccagcaccag cgtggccacc gcgccgtcag acagcgccgc ctgtagcgcg
     1861 gcgacgacgt acccggctgc cgcggccatg ccggcgacga cgcgcacgtc cggcgttggc
     1921 gtggatggca tgtttgtagt ggtcataggc tttaggacat taacaaatta acaggcattt
     1981 atgataca
//
LOCUS       DAOSMQ010000564         1985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_57101_length_1985_cov_4.769948, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000564 DAOSMQ010000000
VERSION     DAOSMQ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57101_length_1985_cov_4.769948"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PK829_14205"
     CDS             <1..971
                     /locus_tag="PK829_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOU42416.1"
                     /translation="TAYRDQYPRFMSEFGFQALPPLATIRTYAAEADWNMTAYVMEQH
                     QKNASGNSLMVGQMLDTFRLPDGFDALVYLSMVLQAEGIRYGVEHWRRHRDRVAGTLY
                     WQLNDCWPVASWSSLDYFGRWKALHYAARRFYAPVLLSIEDDPPRQAISVTNDRPERW
                     DGCARWSLETLDGAVLAAGEEPVAAAPLATTTVKALDLVAHVTEANRRSLVFVAELWR
                     DEQPVARQTAFFAVTKHLELADPRIAATCAGDGAGLRITLRGRSLARLVEVSLDGADV
                     VFSDNYFDLPAGRAVTVSCPLPAGWDGQRAAAALRVRSVYDSFQSQ"
     gene            1130..>1985
                     /locus_tag="PK829_14210"
     CDS             1130..>1985
                     /locus_tag="PK829_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU42417.1"
                     /translation="MTNIQSLIAQMTLEEKAALCTGDSAWTTTPVKRLDIPELLVSDG
                     PHGIRRVPDVQSMSIHSMPATCFPTASSLAATWDVALLREMGQALGREALALNVGVVL
                     GPGANMKRSPLCGRNFEYFSEDPYLAGEMAVGLIEGIQSQGVGTSLKHFAANNQECER
                     FSVNAVVDERTLREIYLPAFERAVTIAKPWTVMCAYNRLNGRYGSENQWLLTEVLKGE
                     WGFEGFVVSDWGAVHNRVAALKAGLDLEMPGPKPTRVNAVVEAVRNGDLDEEILDEAV
                     RRILTIVCR"
BASE COUNT          339 a          651 c          642 g          353 t
ORIGIN      
        1 ttaccgccta tcgcgaccag tatccgcgct tcatgtccga gttcggcttc caggcactgc
       61 cgcccctggc caccatccgc acctacgccg ctgaggccga ctggaacatg accgcctatg
      121 tcatggagca gcaccagaag aacgccagcg gcaatagcct catggtcggg caaatgctgg
      181 ataccttccg gctgcccgac ggcttcgacg cgctggtcta cctgagcatg gtgttgcagg
      241 cggagggcat ccgctatggc gtggagcact ggcgacgcca ccgcgaccgc gtggctggca
      301 cgctctactg gcaattgaac gactgctggc cggtggcgtc gtggtcgagc ctcgactact
      361 tcggccgctg gaaggcgctt cactacgccg cccggcgctt ctacgcgccc gtgctgttgt
      421 ccatcgagga cgacccgccg cggcaggcca ttagcgtgac caacgaccgg ccggagcgct
      481 gggacggctg tgcgcgctgg tcgctggaga cgctggacgg cgcggtgttg gcggcgggcg
      541 aggaaccggt ggccgccgcg ccgctggcca cgacgacggt gaaagccctc gatctggtcg
      601 cccacgtgac cgaggccaac cgccggtcgc tcgtcttcgt ggccgaactg tggcgcgacg
      661 agcagcccgt cgcccggcag acggcctttt tcgccgtcac aaagcatctg gaattggccg
      721 acccgcgcat cgccgccaca tgcgccggcg acggggcggg tctgcgcatt actctgcggg
      781 gccgctcgct ggcgcggctg gtggaggtgt cgctcgatgg cgcggacgtc gtcttcagcg
      841 acaactactt cgacctgccg gccggccgcg ccgtcaccgt cagctgtccg ctgcccgcgg
      901 gctgggacgg ccaaagggcc gcggccgctt tgcgcgtgcg ctcggtctac gattcttttc
      961 agtctcagta attgtagggg cgggatggca tcccgccatt aacacgaagt cctggggaag
     1021 agcatctttg ccgcgggttt cacgacaaac ctgttttttc catcatctac catctgtgaa
     1081 atcatgcccc tgaagttcat acctttcgga gcagtagaag gagtcagtca tgactaacat
     1141 tcaatccctt attgcccaga tgaccctgga agaaaaagcg gcgctgtgca cgggcgacag
     1201 cgcctggacg acaacgccgg tcaaacgcct cgacatcccg gaattgctgg tgtccgacgg
     1261 cccacatggc atccgtcgcg tgcccgacgt tcagtcgatg agcatccaca gcatgccggc
     1321 gacctgtttc cccacggcct ccagcctggc ggctacctgg gacgtggccc tgctgcgcga
     1381 gatgggacag gcgctgggcc gggaggcact ggcgttgaac gtgggcgtcg tcctcggccc
     1441 gggagccaac atgaagcgct cgcccctttg cggccgcaac ttcgagtact tctccgaaga
     1501 cccctatctg gccggcgaga tggccgttgg cctcattgag ggtattcaga gccagggcgt
     1561 cggcacatcg ctcaagcact tcgccgccaa caatcaggag tgcgagcgct tctcggtcaa
     1621 cgccgtggtg gacgagcgca ccctgcgcga gatctatctg cccgcgttcg agagggccgt
     1681 taccatagcc aagccgtgga cggtcatgtg cgcctacaac cggctcaacg gccgctatgg
     1741 ctccgaaaat caatggctgc taaccgaggt gctgaaaggt gagtggggct tcgagggctt
     1801 tgtcgtctcc gactggggcg cggtccacaa tcgggtggcg gctctgaaag ccggactaga
     1861 cctggagatg cccggcccca aacccacgcg cgtcaacgct gtcgtcgagg cggtgcgcaa
     1921 cggcgacctg gacgaggaaa tcctcgacga agcggtgcgg cgcatcctga ccatcgtctg
     1981 ccggg
//
LOCUS       DAOSMQ010000565         1978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_57395_length_1978_cov_3.666667, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000565 DAOSMQ010000000
VERSION     DAOSMQ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57395_length_1978_cov_3.666667"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..>1978
                     /locus_tag="PK829_14215"
     CDS             160..>1978
                     /locus_tag="PK829_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42418.1"
                     /translation="MKTTRHLLAGIFLISMGALALEVILTRIFSVTMWYHFAFLAISM
                     ALMGSATAGVVLYFFPRLREPESARLWIGRAAATLAVAVPIVFMAYLRIPFRPVLMNR
                     AGAFSFEQLGWLALIYVLLSLPFFLSGSVLALSLAGWPGDAGRVYAADLIGAAVGCLL
                     SVAALSRLGGVNALLLVGVVLALAAAAFVLGQRRQRLVPAGLALLFTAGLLASNVAAP
                     WLRIVVNKAGGAEPPIVYERWNIHSRVTVYEPENYPFFWGVSPVKWDEAVAAGGLWQH
                     ALLLIDAVAGTPIQRFTGDLAQVDFLRFDLTAIAYQLSNAPQTLVIGPGGGRDVLTAL
                     ASGAPHVTAVEVNPAVIEAVRGPFAEFAGNLYSRPDVEVVVADARGTIDRSADRYDII
                     QASLIDTWAAGGSGAFALSENSLYTREAFAGYFDHLSDDGILSISRWYLPGRPAETLR
                     LVATGMAGWQTAGVADPRGHVAVIAMPNRATAQTEGLSTTLFKRQPFTAAEVAHLQEV
                     AAAYGYEVLYAPGLAPVEEVGQFITDADHDAFIRAYPLDISPATDNRPFFFNLILLGH
                     LLDPALSGSGVYRTSMEAIIILFAVIVVTATLSALFILVP"
BASE COUNT          252 a          744 c          634 g          348 t
ORIGIN      
        1 ctgcgcgtcg tggagacgtt cgtcggcggg acgtcggtct atcgcgcgga ctgacatcag
       61 gtgacgccgc cgccgcgcgg ggctacaatc ccgctacgcg gcgaatggcc gtcacgcgcg
      121 gccgcgccgg gatgctttcc gcgagggttc acaccaccga tgaaaacgac gcgacacctt
      181 ctggccggca tcttcctgat cagcatgggc gcgctggcgc tggaagtcat cctgacccgc
      241 atcttcagcg tgacgatgtg gtaccacttc gccttcctgg cgatctcgat ggccctcatg
      301 ggcagcgcca cggctggagt cgtgctctat ttcttccccc gcctgcgcga accggagtcg
      361 gcgcggctat ggatcggccg ggccgccgcc accctggccg tggccgtgcc catcgttttc
      421 atggcctacc tgcgcatccc tttccggccg gtgctgatga accgcgccgg ggcgttctcc
      481 ttcgagcagc tgggctggtt ggcgctgatc tacgttctgt tgtcgctgcc cttctttctg
      541 tccggctcgg tgctggccct gtcgctggcc ggctggccgg gcgatgccgg gcgcgtctac
      601 gctgccgacc tgatcggcgc ggccgtgggc tgcctgctca gcgtcgccgc cctgtcgcgg
      661 ctgggcggcg tcaatgccct gctgctcgtc ggcgtcgtcc tggccctggc cgcggcggcg
      721 ttcgttctgg gtcagaggcg acagcggctc gtgcccgccg ggctggcgct gctcttcacc
      781 gccgggctgc tggccagcaa cgtggccgcg ccctggctgc gcatcgtcgt caacaaggcc
      841 ggcggcgcgg agccacccat cgtctatgaa cgctggaaca tccattcccg cgtcacggtc
      901 tatgagccag agaactaccc cttcttttgg ggcgtgtcgc cggtcaagtg ggacgaggcc
      961 gtggccgccg gcgggctgtg gcagcacgcc ctgctgctca tcgacgccgt ggccggcacg
     1021 cccatccagc gcttcaccgg cgacctggcc caggtggact tcctgcgctt cgacctgaca
     1081 gccatcgcct accagctcag caacgcgccg caaacgttgg tcatcggccc cggcggcggc
     1141 cgtgacgtgc tgaccgccct ggcctccggc gcgccccacg tcacggccgt cgaggtcaac
     1201 ccggcggtca tcgaggccgt gcgcggcccc ttcgccgagt tcgccggcaa cctctacagc
     1261 cggcccgatg ttgaggtcgt cgtcgccgac gcccgcggca ccatcgaccg cagcgccgac
     1321 cgctacgaca tcatccaggc gtcgctcatc gacacgtggg cggccggcgg ctccggcgcg
     1381 ttcgccctca gcgagaacag cctctacacg cgcgaggctt tcgccggcta cttcgaccac
     1441 ctcagcgacg acggcatcct gagtatttcg cgctggtacc tgcccggtcg cccggcggag
     1501 acgctgcggc tggtggccac gggcatggct ggctggcaga ccgccggcgt ggccgacccg
     1561 cgcggccacg tcgccgtcat cgccatgccc aaccgcgcca ccgcccagac cgagggcctt
     1621 tcgacgacgc tcttcaaacg gcagcccttc acggccgcgg aagtagccca cctgcaggag
     1681 gtggccgcgg cctatggcta cgaggtgctc tatgcgccgg ggctggctcc ggtggaggaa
     1741 gtcggccagt tcatcaccga cgccgaccac gatgccttca tccgcgccta cccgctcgac
     1801 atctccccgg cgaccgacaa ccggccgttc ttcttcaacc tcattctgct gggccacctg
     1861 ctcgacccgg ccctcagcgg cagcggcgtc taccgcacca gcatggaggc catcatcatc
     1921 ctcttcgccg tcatcgtcgt cacggcgacg ctgagcgccc tgttcatcct ggtgcccc
//
LOCUS       DAOSMQ010000566         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_57478_length_1976_cov_3.930245, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000566 DAOSMQ010000000
VERSION     DAOSMQ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57478_length_1976_cov_3.930245"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PK829_14220"
     CDS             complement(<1..765)
                     /locus_tag="PK829_14220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOU42419.1"
                     /translation="MEIPIETTITTVTVYPDRARVTCRGRAEVAPGQHQLIVGELPLA
                     LEPESVRVGGAGTARVQLRGVDVQRRHYAQAPAANVLALEEQIKGLQDELQGLDDRQA
                     VKQASIGHLDGLRKATTEYAWGLARGRSTADNQAALMRFFEEEDTRLRADLRALGGEG
                     RSLQEQLDKLRAELDQLHSARPRQRFEARVEVEVLSAGEFAVEVTYVAGRAGWRPLYD
                     MRLEQPQTAGGAASVALTALAEITQNTGQDWRGVGLT"
     gene            complement(897..>1976)
                     /gene="trmFO"
                     /locus_tag="PK829_14225"
     CDS             complement(897..>1976)
                     /gene="trmFO"
                     /locus_tag="PK829_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOU42420.1"
                     /translation="REAFAQLVTERIAGHPRITLVHEEVTALPDGPAIIATGPLTSPA
                     LAEVIGRLSGQDYLYFYDAVAPIVQAETIDMTIAFRQSRYDRGEQEEGDYINCPMTRE
                     EYEAFVDALLAAETIALKQFEREDAHFFEGCMPVEALARRGREALAFGPLRPVGLLDP
                     RTGKRPYAVVQLRQDNLIGSLYNLVGFQTNLKWPDQKRVLRLIPGLGAAEFVRYGLLH
                     RNTYINAPALLQPTLQYRNRADLFFAGQIVGVEGYVGNAATGLLAGVNAARLLTGGHP
                     LILPPATMLGALCHYVTHAAVKDFQPMKANFGILPPLPPRPGKEDRGHLYADRALTAL
                     RRFARAHGLRYDGSLAAPEAHVPSP"
BASE COUNT          307 a          651 c          696 g          322 t
ORIGIN      
        1 ggtcaggccg acgccgcgcc agtcctggcc cgtgttctgc gtgatctccg ccagagccgt
       61 cagagccacg ctggccgcgc cgccggcggt ctgcggctgt tccaggcgca tgtcgtacag
      121 cggacgccag ccggcccggc cggcgacgta ggtcacttcg acggcaaact cgccggccga
      181 cagcacctcg acctcgacac gggcctcaaa gcgctggcgc ggccgggcgg aatgaagctg
      241 atctagctcg gcccgaagct tgtccaactg ctcttgcagg ctgcggccct cgccccccag
      301 ggcgcgcagg tcagcgcgca ggcgggtgtc ctcctcctca aagaagcgca tcagcgcggc
      361 ctggttgtcg gccgtgctgc ggccgcgggc cagcccccag gcatactcgg tcgtcgcctt
      421 gcgcagaccg tccagatggc caatcgacgc ctgtttgacc gcttgccggt catccagacc
      481 ttgcagttcg tcctgaagtc ccttgatctg ttcttccagg gccaacacat tggccgcggg
      541 ggcctgggca tagtggcggc gctgcacgtc gacgccgcgc agttgcacgc gggccgtgcc
      601 cgcgccgccg acgcgcaccg actccggctc cagcgccagc ggcagctcgc cgacgatgag
      661 ttggtgctga ccgggcgcga cttcggcccg gccgcggcag gtcacgcgcg cccggtcagg
      721 gtagacggtg accgtagtga tggtggtttc aattggaatt tccatgacgc tctcttgtgg
      781 ttactcggtg acggtacaac tggacaacgg gcacactccc cctgctggcc gttccctcgc
      841 cccttaggag aaggtcaggg agagggcctc ctatcccctc gccctttggg agagggttag
      901 ggagagggca catgtgcctc cggtgcagcc aggctcccat cgtaccgcag gccatgcgcg
      961 cgcgcaaagc ggcgcagggc ggtcagggcg cggtcggcat agagatggcc gcggtcttcc
     1021 ttgcccggcc gcggcggcag cggcggcagg atgccgaagt tggccttcat cggttggaag
     1081 tccttcacgg cggcgtgggt gacatagtgg cacagcgcgc cgagcatcgt cgccggcggc
     1141 aagatgagcg ggtggccgcc ggtcagcagc cgggcggcgt tgacgccggc cagcagcccc
     1201 gtggcggcgt tgccgacgta gccctccacg ccgacgatct gcccggcaaa gaacaggtcg
     1261 gcccggttgc gatactggag cgtcggctgc aaaagggccg gggcgttgat gtaggtgttg
     1321 cggtgcagca ggccatagcg gacgaactcg gccgccccca ggccggggat caaccgcagc
     1381 acgcgcttct ggtcgggcca cttcagattg gtctggaagc caaccaggtt atagagcgag
     1441 ccgatgaggt tgtcctgccg caactggacg acggcgtagg gccgtttgcc cgtgcgcggg
     1501 tcgagcaggc ccaccggccg caatgggccg aaggccagcg cctcgcgccc gcgacgcgcc
     1561 agcgcctcga cgggcatgca gccctcgaag aagtgggcgt cttcgcgctc gaactgcttg
     1621 agggcgatgg tctcggccgc cagcagggca tcgacaaagg cctcgtactc ctcgcgcgtc
     1681 atcgggcagt tgatatagtc gccttcctcc tgctcgccgc ggtcgtagcg cgattggcgg
     1741 aaagcgatgg tcatgtcgat tgtctccgcc tggacaatgg gagcgacagc atcgtaaaag
     1801 taaaggtagt cctgcccgct gagtcggcca ataacctcgg ccagcgctgg cgaggtcagc
     1861 gggccggtag cgataatagc tgggccgtcg ggcagggcgg ttacctcttc gtggacgaga
     1921 gtgatgcgcg ggtggccggc aatgcgctcg gtgaccagtt gggcgaacgc ctcgcg
//
LOCUS       DAOSMQ010000567         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_57830_length_1968_cov_3.829064, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000567 DAOSMQ010000000
VERSION     DAOSMQ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57830_length_1968_cov_3.829064"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK829_14230"
     CDS             complement(<1..211)
                     /locus_tag="PK829_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42421.1"
                     /translation="MRGKVIFLILGLLLVASLVAPTTHAADIRSGERVIIAADEVIDD
                     DLIVSAQYIEVNGTVTGDLVATGTII"
     gene            582..1004
                     /locus_tag="PK829_14235"
     CDS             582..1004
                     /locus_tag="PK829_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42422.1"
                     /translation="MATQPALPRLLEGAPAAPQRAPARRARTRAVTDEADVPVQQHLP
                     GLLPQELDVLELEGLAPDLQHELQEVRLVMYRLLRLWDDAEPPLAPEEARRLAALVFT
                     GARTVAHLLNHQAGAGGDARGWLKGALDQVAADYGLDL"
     gene            988..>1968
                     /locus_tag="PK829_14240"
     CDS             988..>1968
                     /locus_tag="PK829_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42423.1"
                     /translation="MASTSSRERDAAERARAAAYLLGDVGRFSRHVIGLPLYDYQVEP
                     LRAVVDSVLNQRGREFLLVFPRQSGKNEAVAQLLVYLLTVLQRAGGQIVYGAVGDGLG
                     RGMRRLEQRLDTPWTRSQWRREARPARYTLGRAAVVFLSSHPRAASRGETADHLLVID
                     EAQDQDAAHIESVFTPMRAAHNATAVYLGTVRQTTDFLWVKKGELESATAADGVQRVF
                     LVDPATVATENAAYGAFLDEQVRRYGRQHPIVAAEYFLEPHDGRGGLFPAWRLALMGG
                     DHARLRAPRPDERYVATIDLAGQDEAATTSEATLRNPGRDYTVATIFRLSE"
BASE COUNT          328 a          680 c          665 g          295 t
ORIGIN      
        1 cgataatcgt gcccgtggcc accagatcac cggtcaccgt gccattgacc tcgatgtact
       61 gggcgctgac gatcagatcg tcgtcgatga cctcatcggc ggcgatgatg acccgctcgc
      121 cgcttcggat gtcggccgcg tgggtggtcg gagctaccag gctggcgacg agcagcagcc
      181 ctaggatcag aaagattact ttgcctcgca tgacgaacct ccaagtaagt gcccaagaat
      241 cactccagca ggtactcgcc cgcatccgcg gcagcggacg ccgcaccggc atctacaggc
      301 gggtgagaca gaatgactct agcaagtact gtacgcccaa agcgtctggc cgtcaccttt
      361 tgcgcccgcg agacaacccc gatttctaaa atcgaacagc ggcaggtaga acggctgtgc
      421 taggatgctt gcaaacatgt gccctggagg aatgtgttat gacaactagc gttcactgtg
      481 aagctctcac tcggcgcggc cggccctgcc gcaacgcggc ccggccccac agccagccgc
      541 cccgttgtgc ccgccatcgc gcccaggcca acgatccaga cgtggccaca cagccagcct
      601 tgccccgtct gttggaggga gcaccggcag cgccgcagcg cgccccggcc cgccgtgccc
      661 gaacccgcgc ggtgacggat gaggccgatg tgcccgtgca gcaacacttg cccggtctct
      721 taccgcaaga gctggatgtg ctggagttag aggggctggc ccctgacttg cagcacgagt
      781 tgcaggaggt gcgcctggtc atgtaccgcc tgttgcggct gtgggacgac gccgagccgc
      841 ccctggctcc ggaggaggca cgccggctgg cggccctggt cttcaccggc gcgcggacgg
      901 tggcccactt gctcaaccac caggccggtg cgggcggcga cgcgcgcggc tggctgaagg
      961 gggcgctgga tcaggtggca gccgactatg gcctcgacct ctagccggga gcgggacgcc
     1021 gccgagaggg cgcgggcggc ggcctatctg ctgggcgatg tcggccgctt ctcgcgccac
     1081 gtcatcggtt tgccgctgta tgactaccag gtcgagccgc tgcgggccgt ggtcgactcg
     1141 gtgctcaacc agcgcgggcg cgagttcctg ctggtcttcc cgcgccagtc gggcaagaac
     1201 gaggccgtag cccaactgct cgtttacctg ctaaccgttt tgcagcgggc aggcggccag
     1261 atagtctacg gagcggtcgg cgacggtctg gggcggggga tgcggcggct agagcagcgc
     1321 ctcgacacgc cctggacacg cagtcagtgg cggcgggaag cgcggccggc acgctacacc
     1381 ctgggccggg cggcggttgt cttcctgagc agccacccgc gcgccgccag ccgtggcgag
     1441 acggccgacc acctgctggt catcgacgag gcccaagacc aggacgcggc ccacatcgaa
     1501 tcggtcttca cgcccatgcg cgccgcccac aacgccaccg ccgtctatct gggcacagtg
     1561 cggcagacaa ccgactttct gtgggtcaag aagggtgaac tggaaagcgc cacggccgcc
     1621 gacggcgtcc agcgcgtctt cctggtagac ccggcgacgg tggcaaccga aaacgccgcc
     1681 tacggcgcgt tcctggacga gcaggtgcgg cgctatggcc ggcagcaccc catcgtggcc
     1741 gcggagtact ttctggagcc acacgacggc cgcggcggcc tcttcccggc ctggcggctg
     1801 gccctgatgg gcggcgacca cgctcgcctg cgcgcccccc ggccagacga gcgctacgtg
     1861 gccaccatcg acctggccgg gcaggacgag gcggcgacga ccagcgaggc gacgctgcgc
     1921 aaccccggtc gcgactacac ggtggcgacc atcttccgcc tgtccgag
//
LOCUS       DAOSMQ010000568         1963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_58050_length_1963_cov_5.148323, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000568 DAOSMQ010000000
VERSION     DAOSMQ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58050_length_1963_cov_5.148323"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..982
                     /locus_tag="PK829_14245"
     CDS             179..982
                     /locus_tag="PK829_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOU42424.1"
                     /translation="MNNQTNLPAIDITDLTVAYQDKPVLWDVDLQVPPGVLLAIVGPN
                     GAGKTTLIKSVLGLIKPVAGQTLIFGAPYATQRRLVAYVPQRGSVDWDFPTSVLDVVM
                     MGRYGRLGWLRRPSRDDREAALAALEKVGMARYADRQISQLSGGQQQRTFLARALVQD
                     AQVYFMDEPFQGVDATTERAIVELLKELRGAGKSVIVVHHDLQTVPEYFDWVLLLNIR
                     RIAAGPVGVVFTEENLRRAYGGRVGFLGGNGHSEPSPAVAPRSAENPAR"
     gene            994..>1963
                     /locus_tag="PK829_14250"
     CDS             994..>1963
                     /locus_tag="PK829_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOU42425.1"
                     /translation="MDLLNSLLFDYTIRTVALGAAVLGIVAGALGSFALLRRQSLLGD
                     AMSHAALPGVLLAFMLTGSKAPVALVLGAAVAGVTATFLLLAISRYSRIKEDAALGVI
                     LSVFFGFGLLLLTFLQRNPRAAQAGLNKFLFGQAATLLTRDVVVMALFGGAALLVVAL
                     FWKEFKLLSFDRDFGASLGYPMTLLDALLTILLVIGIVIGLQAVGVVLMSALIVAPAA
                     AARQWTDRLSTMLVVASAFGALAGVAGALISSLGTGLSTGPVVVLVISVIVLLSLLFG
                     AARGLVWTWLRRRRSRRTLRTQAVLTNLHDLAREHRDPHYAHPVATL"
BASE COUNT          283 a          715 c          622 g          343 t
ORIGIN      
        1 ttctggacac gccgctcaag aaggtgcgac gcacttcaga aagtgcgacg caccgaccga
       61 ctcggcagtg cctggcactt tctggagtgc ctggcacttt ctggacacgc cgctcaagaa
      121 ggtgcgacgc acttcagaaa gtgcgacgca ccgggagatt aatcaagaga catcatctat
      181 gaacaaccag accaacctac ccgccatcga catcaccgat ctgaccgtcg cctatcagga
      241 taagcccgtt ctctgggacg tcgacctgca agtgccgccg ggcgtgctgc tggccatcgt
      301 cggccccaac ggcgcgggca aaacgacgct gatcaagtcc gtcctcggcc tgatcaagcc
      361 ggtcgccggg cagacgctga tcttcggcgc gccctatgcc acacagcgcc gcctggtcgc
      421 ctatgtgccc cagcgcggca gcgtcgattg ggacttcccc accagcgtcc tcgacgtggt
      481 catgatgggg cgctacggcc gtctcggctg gctgcgccgc cccagccgcg acgaccgcga
      541 ggccgccctg gccgcgctgg agaaggtcgg catggcccgc tacgccgacc ggcagatcag
      601 ccagctctcc ggcggccagc agcagcgcac cttcctggcc cgcgccctcg tccaggacgc
      661 gcaggtgtac ttcatggacg agccgttcca gggcgtcgac gcgaccaccg agcgggccat
      721 cgtcgaactg ctcaaggagc tgcgcggcgc gggcaagtcc gtcattgtcg tccaccatga
      781 cttgcagacc gtgccggagt atttcgactg ggtgctgttg ctcaacatcc ggcgcatcgc
      841 cgcggggccg gtgggcgtcg tcttcaccga ggagaacctg cgccgggctt atggcggccg
      901 tgtcggcttc ctgggtggca acggccacag cgaacccagc ccggccgtcg ccccgcgcag
      961 cgccgagaac ccggcccgct gaggagcgcc atcatggatc tgctgaatag tctcctcttc
     1021 gactacacca tccgtaccgt ggccctgggc gcagccgtgc tgggcattgt cgccggggcg
     1081 ctgggcagtt tcgccctgtt gcgccgccag agcctgctgg gcgacgccat gtctcacgcc
     1141 gccctgcccg gcgtgctgct ggctttcatg ctgaccggca gcaaggcccc cgtggccctg
     1201 gtgctgggcg cggccgtggc cggcgtcacg gccaccttcc tgctgctggc tatcagccgc
     1261 tatagccgca tcaaagaaga cgccgccctg ggcgtcatcc tgtccgtctt cttcggcttt
     1321 ggcctgctgc tgttgacctt cctgcaacgc aacccgaggg cggcccaggc cggcctgaac
     1381 aagttcctct tcggccaggc ggccacgctg ctgacccgcg acgtggtcgt catggccctg
     1441 ttcggcggcg cggcgctgct ggttgtggcc ctcttctgga aggagttcaa gctgctcagc
     1501 ttcgaccgcg acttcggggc cagcctcggc taccccatga cccttctcga cgcgctgctg
     1561 accattttgc tggtcatcgg catcgtcatt ggcctgcaag ccgtcggcgt cgtgctcatg
     1621 agcgcgctca tcgtcgcccc ggccgcggcc gcgcgccagt ggaccgaccg gctgagcacc
     1681 atgctggtcg tcgcctcggc gttcggggcg ctggccggcg tcgccggcgc gctcatcagc
     1741 agcctgggca cgggcctgtc caccggcccg gttgtcgtcc tggtgatcag cgtcatcgtt
     1801 ctgctgtcac tgctgttcgg cgcggcgcgg gggctggtct ggacgtggct gcgccgccgc
     1861 cgcagtcgcc gcacgctacg cacccaggct gtcctgacca acctccacga cctggcccgc
     1921 gagcaccgcg acccgcacta cgcccatccc gtggccacgc taa
//
LOCUS       DAOSMQ010000569         1961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_58142_length_1961_cov_3.746590, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000569 DAOSMQ010000000
VERSION     DAOSMQ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58142_length_1961_cov_3.746590"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PK829_14255"
     CDS             complement(<1..337)
                     /locus_tag="PK829_14255"
                     /inference="COORDINATES: protein motif:HMM:NF021158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HOU42426.1"
                     /translation="MKNNKKLWVATLLLLVVVALAACGGAQQAAEQAEIAAPGDSLVS
                     GTVTYLDRSALPAGAIITVELADVSIADAPANVLATQTIPVGDQQAPFPFELAYDPTA
                     VNPASRYVVQ"
     gene            681..>1961
                     /locus_tag="PK829_14260"
     CDS             681..>1961
                     /locus_tag="PK829_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU42427.1"
                     /translation="MSDDATSAPFVPADEAAALRDAMLTLNDQRPLLGDAVVDAAIMA
                     MRERLAALEAAALADRRRKQVTVLFGDIVGFTALSEGRDAEVVAQIINTLWEGVDAAI
                     LAHGGRIDKHVGDAVIAVWGGAVAREDDPESAVHAALAMHAATEACCRLYRAPISLRI
                     GIHTGPALTGAVGAGGETTVVGETVTLADRLQHAAPADGIVISPATYRQVRGIFDVQP
                     AESAARSVGLPAAYLVQAAKQRAFRVATRGIEGIETRMVGRETELGVLQEAFAAIVAE
                     RWPRLLAVIGEAGIGKSRLLYEFDSWVELRPEEVFYYKGRAAASATPFGLFHDLFANR
                     FEILDSDPPEVALGKFRAGFALGRVESLADAGALADAAAIVGQWLGFDFDSSPAVARL
                     LGSPEFGRAAQAHLFRYFRARAAVDPLVILCEDLH"
BASE COUNT          289 a          686 c          653 g          333 t
ORIGIN      
        1 cctgcaccac gtagcgcgag gcggggttga ccgccgtcgg gtcataggcc agttcaaacg
       61 ggaacggcgc ttgttggtcg ccgacaggga tcgtctgcgt ggccagcacg ttcgcggggg
      121 cgtcggcgat agacacgtcc gccagttcca cggtaatgat cgcgccggcc ggcagcgccg
      181 agcggtccag ataagtcact gtgccactga cgaggctgtc gccgggggca gcgatttcgg
      241 cctgttcggc cgcctgttgc gccccgccgc aggccgccag tgccactacc accagcagta
      301 gcagcgttgc cacccataac ttcttattgt tcttcattgc cagctccttc tccaggcgct
      361 ctgtactgac tcagcctgtt tttcgcagtc agcgcccccg acgtctgtct aagtataagc
      421 gccgtcaggg gttctgacaa acggggccct gcggccggcg gggcttcgcc aaagtcgtct
      481 aacgaccatt gacctgacat gtgggcgggg ccggtcgttc gctggtgaaa ggcccactcg
      541 tggccgcgca cctgtcaggt cggcgcgccg ggagtgactt tggaatagcc ctactgacaa
      601 acggggccat gcggccggcg gctaatctgg tacaatacgc tcaggcaaag atcccccacc
      661 atcgagaccg cttacccgcc atgagtgatg acgccacatc tgcccccttt gttcccgccg
      721 acgaggccgc cgccctgcgc gacgccatgc tgaccctgaa cgaccagcgc cccctgttgg
      781 gcgacgccgt tgtcgatgcc gccatcatgg ccatgcgcga gcgcctggcc gcgttagagg
      841 cggctgccct ggccgaccgc cgccgcaagc aggtgacagt gctcttcggc gacatcgtcg
      901 gtttcaccgc cctatcagag gggcgcgacg ccgaggtggt ggcccagatc atcaacacgc
      961 tctgggaggg cgtcgatgcg gccatcctgg cccacggcgg gcgcatcgac aagcatgtcg
     1021 gcgatgccgt catcgccgtg tggggtggcg ctgtggcccg cgaggacgac ccggagagcg
     1081 ctgtccacgc cgcgctggcc atgcacgccg ccaccgaggc ctgctgtcgc ctctaccgcg
     1141 cgcccattag cctgcgcatc ggcatccaca ccggcccggc gctcaccggc gcggtgggcg
     1201 cgggcggcga gacgacggtc gtaggcgaaa cggtgaccct ggccgatcgc ctgcagcacg
     1261 ccgcgccggc cgatggcatc gtcatctccc cggcgaccta ccggcaagtg cgcggcatct
     1321 tcgacgtgca gccggccgaa agcgccgccc gaagcgtggg gctgccggcg gcctatctgg
     1381 tgcaggcggc caagcagcgc gccttccgtg tcgccacccg cggcatcgag ggcattgaga
     1441 cgcgcatggt cggccgcgag acggaactgg gcgtgctaca agaggctttt gccgccatcg
     1501 tggccgagcg ctggccgcgc ttgctggcgg tgatcggcga ggcgggcatc ggcaagtccc
     1561 gcttgctata cgaattcgat agctgggttg agctgcggcc ggaggaggtc ttctattaca
     1621 aggggcgcgc cgcggccagc gcaaccccct ttggcctgtt ccatgacctc ttcgctaacc
     1681 gtttcgagat cctggacagc gacccgcccg aggtcgccct gggcaagttc cgagccggct
     1741 ttgccctggg gcgcgtcgag tcgttggccg acgccggcgc gctggccgat gccgcggcca
     1801 tcgttggcca gtggctcggc ttcgacttcg acagcagccc ggccgtggcc cgtctgctgg
     1861 gcagcccgga gttcggccgc gccgcgcagg cgcacctctt tcgctacttc cgcgcccgcg
     1921 ccgccgttga cccgctggtc atcttgtgcg aagacctcca c
//
LOCUS       DAOSMQ010000570         1958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_58285_length_1958_cov_3.271151, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000570 DAOSMQ010000000
VERSION     DAOSMQ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58285_length_1958_cov_3.271151"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PK829_14265"
     CDS             complement(<1..1025)
                     /locus_tag="PK829_14265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU42428.1"
                     /translation="MRIGLVTGEYPPMEGGVGAFTEQLARALAGRGHVLHIITARSAR
                     PVDAPRRASALFQPYDLGFAQLHARVGRWRWPSLATVADIVLRYDLEVVNLQYQAAAY
                     NMRAVAIHFLPWRLRGVAPTVVTFHDLRVPYLFPKAGGLREAAVRGLARRATGGIATN
                     AADLAQLASWTDAPLCRIPIGSNIEAREPNHVEIEEVRVGLGLPAGAVLLGYFGFLNE
                     TKGADTLIDALARLDGRTHLVFIGGQTGASDPQNNETFLRGLREQIDRLGLAGRVHWT
                     GFLPPGRVSAHLAAADLLVMPYRDGVSLRRGTLMAALAHGRPLITTAPGEADAAPELR
                     HGDNVWFV"
     gene            complement(1029..>1958)
                     /locus_tag="PK829_14270"
     CDS             complement(1029..>1958)
                     /locus_tag="PK829_14270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU42429.1"
                     /translation="PTMLSILIVNWNVRDLLRDCLRSIERGRGELEVEVIVVDSASSD
                     DSAAMVAAEFPWVTLLPQADNVGFPRGNNIALARARGDALLLLNPDTVIRDDALPVLV
                     AYLAAHPDVGAVGPQLLNPDGSVQSSRRRFPTLATGFLESTWLEGLAPGVLRRYYALD
                     LPDDATADVDWLTGACILVPRAVYERVGGLDEGYYMYSEELDWCRRIRAAGWLVVYLP
                     AARVAHHVGKSSEQAVTARHINFQRAKLRYFRKYHGRAAAGLLRLFLLASYGWQLLLE
                     AAKGALGSQPELRRQRVRAYRDVLRSGLRPAGY"
BASE COUNT          329 a          651 c          715 g          263 t
ORIGIN      
        1 acgaaccaga cgttgtcgcc gtggcgcagt tccggcgcgg cgtcggcctc gcctggcgcg
       61 gtggtgatca gcggccggcc gtgggccaga gcggccatga gtgtcccccg gcgcagggaa
      121 acgccgtcgc ggtagggcat gaccagcaga tcggccgcgg ccagatgggc cgagacgcgc
      181 cccggcggca ggaagcccgt ccagtgaacg cgcccggcca gccccaaccg gtcgatctgc
      241 tcgcgcaggc cgcgcaggaa ggtctcgttg ttctgcgggt cgctggcccc ggtctgcccg
      301 ccaatgaaga ccagatgggt gcgcccatcc agccgcgcca gggcgtcgat gagcgtgtcg
      361 gcgcccttgg tttcgttcag aaagccgaag tagcccagta gcaccgcgcc ggccggcagc
      421 cccaggccaa cgcgcacctc ttcgatctcg acgtggttgg gttcgcgagc ctcgatattg
      481 ctgccaatgg ggatgcgaca cagcggggca tcggtccagc tggccaactg ggccagatcg
      541 gcggcattgg tggcaatgcc accggtggcc cgccgcgcca gaccgcgcac ggccgcctcg
      601 cgcagcccgc ccgctttggg gaacaggtag ggcacgcgca ggtcgtggaa ggtgacgacg
      661 gtcggggcga cgccgcgcaa gcgccagggc aggaagtgga tggccacggc gcgcatgttg
      721 taggccgcgg cctgatattg caggttaacg acttcgagat cgtagcgcag gacgatatcg
      781 gcgaccgtgg ccagcgacgg ccagcgccag cggccgacgc gcgcgtgcag ttgggcaaaa
      841 ccgaggtcat agggctggaa cagcgccgac gcgcggcgcg gggcatcgac cgggcgggca
      901 gatcgggcgg tgatgatatg gagcacgtgg ccgcgcccgg ccagcgcgcg ggccaactgc
      961 tccgtgaacg cccccacccc gccctccatg ggcgggtatt cgcccgtcac cagcccaata
     1021 cgcatggcct aataccccgc gggccgcaac ccggagcgca acacgtcgcg gtaggcgcgc
     1081 acgcgctggc ggcgcagttc cggctgactg cccagcgcgc ccttggccgc ttccagtagc
     1141 agttgccagc cgtagctggc cagcaggaag aggcgcagca ggccggcggc ggcgcggccg
     1201 tggtacttgc ggaagtagcg cagcttggcc cgctggaagt tgatgtgccg ggcagtgacg
     1261 gcctgctcac tgctcttgcc aacgtggtgg gcaacccggg ccgcgggcag atagacaacc
     1321 agccagccgg cggcgcgaat gcggcgacac caatccagct cctcggagta catgtagtaa
     1381 ccctcgtcca gaccgccgac gcgctcatag accgcccgcg gcacaaggat acaggcgccg
     1441 gtcagccagt cgacgtcggc tgtggcgtcg tcgggcaggt cgagggcgta gtagcgccgc
     1501 agcacgccgg gagcaagccc ctccagccag gtactctcca ggaagccggt ggccagcgtg
     1561 ggaaagcgac ggcgcgagga ctgcacgctg ccgtcggggt tcagcaactg cgggccgacc
     1621 gcgcccacgt cgggatgggc ggccagatag gccaccaaca ctggcagggc atcatcgcgg
     1681 atgactgtgt cgggattgag cagcagcaga gcgtcgccgc gggcgcgggc cagggcgatg
     1741 ttgttgccgc gcgggaagcc gacgttgtcg gcctgtggca gcagtgtcac ccaggggaat
     1801 tcggccgcca ccatcgccgc gctgtcgtcg ctggaggcgc tgtcaacgac gatcacttct
     1861 acctctagtt cgccccggcc gcgctcgatg gaccgcagac agtcgcgcag caggtcacgg
     1921 acgttccagt tgacgatgag gatggagagc atggtggg
//
LOCUS       DAOSMQ010000571         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_58339_length_1956_cov_4.990005, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000571 DAOSMQ010000000
VERSION     DAOSMQ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58339_length_1956_cov_4.990005"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /gene="recR"
                     /locus_tag="PK829_14275"
     CDS             <1..390
                     /gene="recR"
                     /locus_tag="PK829_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOU42430.1"
                     /translation="PICSDDARDAGLLCVVEEPLDVLAIERARAYNGRYHVLHGAISP
                     VEGIGPEELRVAELAQRVAAGDFREIILATNPTLEGESTALYLQRRLADQKVRITRLA
                     RGLPVGGDLEYTDEITLGRAFEGRQDV"
     gene            491..1894
                     /locus_tag="PK829_14280"
     CDS             491..1894
                     /locus_tag="PK829_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU42431.1"
                     /translation="MIATLDLRDRIVGLDQEVPLLDGRRLPYVNLDNAASTPPLVDVV
                     RAVDAFMPYYASVHRGTGFKSRLSTVAYDQAHAIIGRFVGADPALDTVIFGKNTTEAI
                     NKLAFRLPLADDAVILTTQLEHHSNDLPWRPRAHVVHVRALPDGRLDEDDFDRQLAAH
                     AGRVALVAVSGASNVSGYIQPIHRLARKAHAAGALILVDAAQLAPHRQVDMRPHDDPE
                     HLDFVALSAHKMYAPFGTGALVGPKEIFLRQPPEYRGGGTVDVVTLDDVHWAGAPDRD
                     EAGSPNVVGAVAMAAAARVLMDVGMDAVAAHEAELVAYALEQLPRVPGVRLYGETDPA
                     RAAEKVGVIPFNLAGMSHFLVAAILGYEGGIGVRSGCFCAHPYVVHLLQLNEAESARW
                     RTNLLAGDKSDMPGMVRASFGGYNTTGDIDRLVEMLGRIARGDYGGDYVLDRHSGEYH
                     PRGFTEPLADYFLLEPL"
BASE COUNT          301 a          719 c          635 g          301 t
ORIGIN      
        1 ccgatctgta gcgacgacgc gcgcgacgcc gggctgctgt gcgtcgtcga ggagccgctc
       61 gacgtgctgg ccatcgagcg cgcgcgggcc tacaacggcc gctaccacgt cctccacggg
      121 gccatctccc ccgtcgaggg catcggcccg gaggagttgc gcgtggccga gctggcccag
      181 cgcgtcgccg ccggcgactt ccgcgagatc atcctggcca ccaacccgac gctggagggc
      241 gaatcgacgg cgctttactt gcagcgccgc ctggccgacc agaaggtgcg cattacccgg
      301 ctggcccgcg gcctgcccgt cggcggcgac ctggaataca ccgacgagat caccctcggc
      361 cgcgccttcg agggccggca ggacgtatag aagaacctca cgcaaagatc gccaagagcg
      421 caaagaagaa ttctttcttg gcgtccttgg cgatctttgc gtgaggttct tatctcttta
      481 ggagaacagc atgatagcca cgctcgacct gcgcgaccgg attgtgggcc tggatcagga
      541 agtgccgctg ctcgacggcc gccgcttgcc ctacgtcaac ctcgacaacg ccgccagcac
      601 accgccgctg gtcgatgtcg tgcgcgccgt ggacgccttc atgccctact acgccagcgt
      661 ccaccgtggc acgggtttca agtcgcgcct cagcacggtc gcctatgacc aggcccacgc
      721 catcatcggc cgcttcgtcg gcgcggaccc ggcgctggac acggtcatct tcggcaagaa
      781 caccaccgag gcgatcaaca agctggcctt ccgcctgccg ctggccgatg acgccgtcat
      841 cctgacgacg caactggagc accactccaa cgacctgccc tggcggccgc gcgcccacgt
      901 cgtccacgtg cgcgccctgc ccgacggccg gctggacgag gacgacttcg accggcaact
      961 ggccgcccac gccggccgcg tggctctggt ggccgtcagc ggcgcgtcga acgtcagcgg
     1021 ctacatccag cccatccacc ggctggcgcg caaggcccac gccgccggcg cgctcatcct
     1081 ggtcgatgcc gcccagttgg ccccccaccg ccaggtggac atgcgccccc acgacgaccc
     1141 ggagcatctc gatttcgtcg ccctgtcggc ccacaagatg tatgccccct tcggcacggg
     1201 ggcgctggtc ggcccgaagg agatcttcct gcgccagccg cccgagtacc gcggcggcgg
     1261 cacggtcgat gtggtcacgc tcgacgacgt gcattgggcc ggcgcgcccg accgcgacga
     1321 ggccggtagc cccaacgtcg tcggcgcggt ggccatggcc gcggcggcgc gggtattaat
     1381 ggacgtgggc atggatgccg tcgccgccca cgaggccgag ctggtggcct atgcgctgga
     1441 gcagttgccg cgcgtgcccg gcgtccggct ctacggcgag accgacccgg cgcgggcggc
     1501 cgagaaggtc ggcgtcatcc ccttcaatct ggccggcatg tcccacttcc tcgtcgccgc
     1561 catcctgggc tacgaaggcg gcatcggcgt gcgcagcggc tgtttttgtg cccacccgta
     1621 tgtcgtccac ctgttgcagc tcaacgaggc cgagtcggcg cgctggcgga ctaacctgct
     1681 ggccggcgac aagagcgaca tgcccggcat ggtgcgggcc agctttggcg gctacaacac
     1741 caccggcgac atcgaccggc tggtggagat gctcggccgc atcgcccgcg gcgactacgg
     1801 cggcgactac gtcctcgacc gccactcggg cgagtatcac ccgcgcggct tcaccgagcc
     1861 gctggccgac tacttcctgc tcgaaccgct ctgagcgtag cgcaggatgt tatcctgcat
     1921 gctgtggttg ccaggacgcc ggttaacaac ccgcgc
//
LOCUS       DAOSMQ010000572         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_58345_length_1956_cov_4.068911, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000572 DAOSMQ010000000
VERSION     DAOSMQ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58345_length_1956_cov_4.068911"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK829_14285"
     CDS             complement(<1..427)
                     /locus_tag="PK829_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42432.1"
                     /translation="MMRGKVAWTLGGMVVLLAVLVGLVGAAPGAVDAPSLVASPREID
                     FGPVGVGTTSAPIAVSFYNLSDTSFLSPFTGGAVGAPFLRTDECNLSVPPQSNCRMLF
                     TFAPETAGVFTATAEVNTDAGPATIVMHGRGVGPKLSLTP"
     gene            complement(567..>1956)
                     /locus_tag="PK829_14290"
     CDS             complement(567..>1956)
                     /locus_tag="PK829_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42433.1"
                     /translation="APNGLACATHDLHRPYLTDHLGVPYDAIRAGAEPGGVIIYNDGP
                     PEPRPSDDDDGPIHYPPTDGGVIWPGILDELGPGPATGGGGDGRSPLLRPAVLDAVRI
                     PATYRAEYARRLAAAGPLPARQEAAARLLIDGLDVRSVRLLHARQIDRAAGTVAGVKV
                     SPATLAALARLSVASGPAAALPFSAGGQLATPAQTFADAFTPLMDRLSPEATPLPPPS
                     RYALESTLAARRGTAFDPGWVGLSVNSYAVDRVLRAVAGSELMAVEGEATASGRRGAD
                     VVYTYQTAATDATRLSADLTGADGAGPGATVTGLEVTLARGDAAVCYRVSGRMAMQPA
                     RVASGCLPDVAVQTVVTYAGCIGQLTGGTISDADYRDALLYRHFIFLEPVAGGGAVTE
                     FELVSGDPDALTPDGLNPRPLLSAALAGMWAGIARVPVLFDPFYALAPVIDHWEARDG
                     WLNVYERYALIE"
BASE COUNT          306 a          696 c          703 g          251 t
ORIGIN      
        1 gcggcgtcag ggacagcttg ggccccacgc cgcggccgtg catcacaatg gtcgctggcc
       61 cggcatcggt gttgacctcg gccgtggccg tgaagacgcc ggccgtctcc ggcgcgaagg
      121 tgaacaacat gcggcagttg ctttgcggcg gcacagacag gttgcactcg tccgtgcgca
      181 ggaacggcgc gccgacagcg ccgccggtga acggcgacag gaagctggtg tcgctcaggt
      241 tgtagaagct aacggcaatc ggcgcgctgg tggtgccgac gccaacgggg ccgaagtcga
      301 tttcgcgcgg cgatgccacc aggctgggcg catcgaccgc gccgggcgcg gccccgacca
      361 accccaccag caccgccagc agaaccacca tgccgccaag cgtccacgcg acttttcctc
      421 gcatcatcag gagcctccat ctgcgcataa agtgtcgccc ccgccgctga ctatcataag
      481 cgagacggcc gggcgggcac aagcggccga aagacgcgcg ggcgcatggg tgggaagtac
      541 tggcgagcgg ggtggcgcgt cagccgctac tcgataagcg catagcgctc gtacacattc
      601 agccacccat cccgcgcttc ccaatggtcg atgaccggcg cgagggcgta gaaggggtcg
      661 aacagcaccg gcacgcgggc aatacccgcc cacatgccgg ccagggccgc cgacagcagc
      721 ggccgcgggt taaggccgtc cggtgtcagc gcatcagggt cgccgctcac gagttcgaac
      781 tccgtcaccg cgccaccacc ggcgactggc tccaggaaga tgaagtggcg gtagagtagc
      841 gcgtcacggt agtcggcgtc gctgatcgtc ccgccggtca gctggccgat gcagccagcg
      901 taggtcacga ccgtctgcac ggccacgtcg ggcagacagc cggaggcgac gcgcgccggc
      961 tgcatggcca tgcgcccgct gacgcgatag cacaccgccg cgtcgccccg tgccagcgtc
     1021 acctccagtc ccgtcaccgt tgccccgggg ccagccccgt ccgcgccggt cagatcggcc
     1081 gacagccgtg tggcgtcggt ggctgccgtc tgataggtgt agacgacgtc cgcgccgcgg
     1141 cggccggacg cggtcgcctc gccctcgacg gccatcagtt ctgagccggc gacggcgcgc
     1201 agcacccggt cgacggcgta gctgttcacc gacaagccaa cccagcccgg gtcgaaggcc
     1261 gtgccgcggc gagcagcgag ggtactctcc agcgcatagc gcgacggcgg cggcagcggc
     1321 gtcgcctcgg gtgacaggcg atccatcaat ggcgtgaagg cgtcggcgaa ggtctgcgcc
     1381 ggcgtggcca gttggccgcc ggcgctgaag ggcaaggccg ccgccggccc gctggcgaca
     1441 ctcaggcgcg ccaacgccgc cagcgtcgcc ggcgaaacct tgacgccggc cactgtgccc
     1501 gccgcccggt cgatctgccg tgcgtggagc aatcgtaccg agcgcacgtc caacccgtcg
     1561 atgagcagcc gcgccgccgc ctcctgtcgg gccgggagag gccccgcggc agccaggcgg
     1621 cgggcatact cggcgcggta ggtcgccggg atgcgcaccg cgtcgagcac agccggacgc
     1681 agcaacggcg agcgcccgtc gccgccccca cccgtcgccg gccccggccc cagttcgtcg
     1741 aggatgcccg gccagatgac gccgccatcc gtcggcgggt agtggatagg gccgtcgtcg
     1801 tcatcgcttg ggcgtggctc cggcgggccg tcgttgtaga tgatgacgcc gcccggctcg
     1861 gccccggcgc ggatggcgtc gtagggcacg ccgagatggt ctgtgagata gggccggtga
     1921 aggtcgtgcg tggcgcaggc caggccgttg ggcgcg
//
LOCUS       DAOSMQ010000573         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_60475_length_1907_cov_3.078294, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000573 DAOSMQ010000000
VERSION     DAOSMQ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60475_length_1907_cov_3.078294"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="PK829_14295"
     CDS             complement(<1..1362)
                     /locus_tag="PK829_14295"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42434.1"
                     /translation="MTGTRRARLLAPILVLLVLLASAGVAAAHPLGNFTISRYSAVTL
                     GAETAEVLYIVDMAEIPTFQTRQAIDSNADGTISAAEEDAYLATELPALAANLRLTAG
                     GALLPLTPTTHTLSFPPGQGDLPTMRLEVRLSAPLPAGGQALDIAYEDGNFADRLGWR
                     EIVITAGDGSLLSASVPAADVSNQLRDYPADLLQAPLAVSQATARFAPAGAALDGAPS
                     DGATAADPAAATTTSAVVANRFGQDKFADLLQRTLDTPAALAAALLVAIGLGAAHALT
                     PGHGKTIVGAYLVGSRGTARHALFLGVTTTITHTAGVFALGFLVLFASRFVLPEKLYP
                     WLGVLSGLLVVGIGLSILRGHVGHWRARRRGDDHDHGPYHFHFGKGHSHGPDGHTHGG
                     PTHSHAPSALEAPEPAAVVSAAAPRRALAFAGVAAAAPAVATHAHEHGHSHASHDHHH
                     EHDQ"
     gene            complement(1364..>1907)
                     /locus_tag="PK829_14300"
     CDS             complement(1364..>1907)
                     /locus_tag="PK829_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU42435.1"
                     /translation="AAHGDYDEAIRRYEALVAQLPQPNFVAALGDYYALSGDNAAAQK
                     QYDTVAFIHQLDETQQVLYNRQMAIFFANHNIETDTALAYAQGELAARQDIYAYDTLA
                     WALYRAGRFDEAADASQRALAHGTPDAGLYYHAGMIAAAQGDTRVAIAQLQQALDLNP
                     NFDLLQARQAATTLATLKGE"
BASE COUNT          266 a          596 c          753 g          292 t
ORIGIN      
        1 ttggtcgtgc tcgtggtggt gatcatggct ggcatggctg tggccatgct cgtgcgcgtg
       61 ggtggccacc gccggggccg ccgcggccac gccggcgaag gccagcgccc gccgcggcgc
      121 ggcggccgag acaactgccg ccggttcggg cgcttcgagg gcgctgggcg cgtggctgtg
      181 cgtggggccg ccgtgcgtgt ggccgtccgg cccgtggctg tggcccttgc cgaagtggaa
      241 atggtaaggg ccgtggtcgt ggtcgtcgcc gcggcggcgg gcccgccaat ggccgacatg
      301 gccgcgcaga atcgacaggc caatgccgac gacgagcagc ccggacagga cgcccagcca
      361 ggggtagagc ttctccggca ggacgaaccg tgaggcgaac aacaccagga agcccagggc
      421 gaagacgccg gccgtgtggg tgatggtcgt cgtcacgccc aggaagagag cgtggcgggc
      481 tgtaccgcgc gagccgacaa ggtaggcccc gacgatggtc ttgccgtggc cgggcgtcag
      541 ggcatgggcc gcgcccagac cgatggccac cagcagcgcc gcggccagcg ccgccggcgt
      601 gtccagcgtg cgctgcaaga ggtcggcgaa cttgtcctgg ccgaagcggt tggcgacgac
      661 ggctgaggtg gttgtcgccg cggccggatc ggccgccgtg gcaccgtccg acggggcacc
      721 gtccagcgcc gcgccggccg gggcaaagcg cgccgtggcc tggctgacgg ccagcggggc
      781 ttgcagcagg tcggccgggt agtcgcgcag ctggttgctg acgtcggccg cgggcacgga
      841 ggcgctcagc agggaaccat cgccggcagt gatgacgatt tcccgccagc cgaggcggtc
      901 ggcgaagttg ccgtcctcat aggcgatgtc aagcgcctgg ccgccggccg gcagcggggc
      961 cgagagccgc acttccagcc gcatggttgg caggtcgccc tggccggggg ggaaggagag
     1021 cgtgtgggtc gttggcgtca gcggcaagag cgcgccaccg gccgtcaggc gtaggttcgc
     1081 cgccagcgcg ggcagctccg tggccaggta ggcgtcctcc tcggcggcgc taatcgtccc
     1141 gtcggcgttg ctgtctatgg cctgccgcgt ctggaacgtg ggaatctcgg ccatatcaac
     1201 gatatagagc acctcagccg tctccgcccc cagcgtcacg gcgctatagc ggctgatggt
     1261 gaagttgccc agcgggtggg ccgcggcgac gccggcggag gccagcagca ccagcaggac
     1321 gaggatcggg gccagaaggc gggcgcgtct tgtgccggtc atgtcactct cctttcagag
     1381 tggccagggt cgtggcggcc tgccgcgcct ggagaaggtc gaagttagga ttcaggtcga
     1441 gggcctgctg tagctgcgcg atggccacac gcgtgtcgcc ctgcgcggcg gcgatcatcc
     1501 cggcgtggta gtagaggccg gcgtcgggcg tgccgtgggc cagggcgcgc tggctggcgt
     1561 ccgcggcttc gtcgaagcgg ccggcgcgat agagcgccca ggccagcgta tcgtaggcgt
     1621 agatgtcctg ccgcgcggcc agttcgccct gagcgtaggc cagggcggtg tcggtttcga
     1681 tgttgtggtt ggcgaagaag atcgccatct ggcgattgta gagtacttgc tgagtctcgt
     1741 ctaactggtg gatgaaggcc acggtgtcat actgcttctg ggccgcggcg ttgtcgccgc
     1801 tgagggcata gtagtcgccc agcgcggcca cgaagttcgg ctgcggcagt tgggccacaa
     1861 gggcctcata gcgcctgata gcctcgtcgt agtcgccgtg ggcggcg
//
LOCUS       DAOSMQ010000574         1897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_60919_length_1897_cov_5.600977, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000574 DAOSMQ010000000
VERSION     DAOSMQ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60919_length_1897_cov_5.600977"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>1897)
                     /locus_tag="PK829_14305"
     CDS             complement(11..>1897)
                     /locus_tag="PK829_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOU42436.1"
                     /translation="IETALDRLLPRVAKPGRYTGGELNQVVKDWDAVGYRVAMAFPDL
                     YDIGMSNLGWMIHYDIINKHRNLLAERVFCPWDDMEAALRARGIPLFSLETKHPIRDF
                     DMLAMTLPYEQLFTNALNLLDLAGLPVRSADRDASYPLVVAGGHACYNPEPMAPFIDV
                     FVIGEGEEVLLEIIATMRAAAHLDRETQLRHVASITGCYVPRFYDVAYHDDGTIAAIT
                     PNVPEAPPRVLKAIVPVLPPPVTDFIVPFVETVHNRAPIEIMRGCTRGCRFCHAGMVT
                     RPVRERPVEEVLAAMEAILAKTGYEEIALMSLSSSDYTHVLELTRRIGERFGGLGLNI
                     SLPSLRIETVSTQLMDNLGDGKRSGFTLAPEAATEKMRNIINKYVTHEQLLETAREIY
                     RRDWRTIKLYFMIGHPQEEMSDVAAIVDLSRQVLAEGRRFHGNKAAVNVGVSTFIPKP
                     HTPFQWEPMGDMAGIRAKLGYLIAEFKRPGLKLRWNDPDESVFEGMLTRGDRRMAAVV
                     ERAWRKGAKFDAWQDHFRRDAWAEAMAEEGLDAAFFTHRPRPIDEVFPWEHIDVAVTR
                     RFLTGDYLMSQRQETRIDCRHHCFACGILPKLKDLRRETPPEAWECPPVTKVADRRPA
                     LIQL"
BASE COUNT          290 a          592 c          688 g          327 t
ORIGIN      
        1 cgaacgctcg ctacaactga atgagcgccg gccgccggtc ggccaccttc gtcaccggcg
       61 ggcactccca cgcctccggc ggcgtctcgc ggcgcaggtc cttcagcttg ggcaggatgc
      121 cacaggcgaa gcaatggtgg cggcagtcga tgcgcgtctc ctggcgctgg ctcatcaggt
      181 agtcgccggt cagaaagcgg cgcgtcacgg ccacgtcgat gtgctcccag gggaagacct
      241 cgtcgatggg ccgcggccgg tgggtgaaga aggccgcgtc cagcccctct tcggccatcg
      301 cctcggccca ggcgtcgcgg cggaagtggt cttgccaggc atcgaatttg gctcccttgc
      361 gccaggcacg ctcgacgacg gcggccatgc gccggtcgcc gcgggtcaac atgccctcaa
      421 acaccgattc gtcggggtcg ttccagcgca gtttcaggcc cggccgcttg aactcggcga
      481 tcagatagcc cagcttggcg cggatgccgg ccatgtcgcc catcggctcc cactggaagg
      541 gtgtgtgggg cttggggatg aaggtgctga cgccgacgtt gacggcggcc ttgttgccgt
      601 ggaagcgccg cccctcggcc agcacctgcc gcgacagatc gacgatggcc gccacgtcgc
      661 tcatctcctc ctgcgggtgg ccgatcatga agtagagctt gatggtgcgc cagtcgcggc
      721 ggtagatctc gcgggccgtc tccagcagtt gctcgtgggt gacgtacttg ttgatgatgt
      781 tgcgcatctt ctccgtggcc gcttcggggg ccagggtgaa gccgctgcgc ttgccgtcgc
      841 ccaggttgtc catcagttgc gtgctgaccg tctcgatgcg caacgacggc agggagatgt
      901 tcaaccccag gccgccaaag cgctcgccga tgcgccgggt cagttccagc acgtgggtgt
      961 agtcgctgga cgacagcgac atgagggcga tctcctcgta gccggtcttg gccaggatag
     1021 cctccatcgc cgccagcact tcctcgaccg gccgctcgcg caccggccgg gtgaccatgc
     1081 ccgcgtggca aaagcggcag ccgcgcgtgc agccgcgcat gatctcgatg ggggcgcggt
     1141 tgtggaccgt ctccacgaag ggcacgatga agtcggtcac cggcggcggc aggacgggga
     1201 cgatggcctt caggacgcgc ggcggcgctt cgggcacgtt gggcgtgatg gcggcgatcg
     1261 tgccgtcgtc gtgataggcc acgtcgtaga agcgcggcac gtagcagccg gtgatgctgg
     1321 ccacgtggcg caactgcgtc tcgcgatcca ggtgggccgc ggcgcgcatc gtggcgatga
     1381 tctccagcag cacttcttcg ccctcgccga tgacgaagac gtcgatgaac ggggccattg
     1441 gttcggggtt gtagcaggcg tggccgccgg cgacgaccag cgggtagctg gcgtcgcggt
     1501 cggccgaacg caccggcagc ccggccaggt cgagcaagtt cagggcgtta gtgaagagct
     1561 gctcataggg cagcgtcatg gccagcatgt cgaaatcgcg gatggggtgc ttcgtctcca
     1621 gcgagaagag ggggatgccg cgggcgcgca gggccgcctc catgtcgtcc caggggcaga
     1681 agacgcgctc ggccagcagg ttgcggtgct tgttgatgat gtcgtaatgg atcatccagc
     1741 cgaggttaga catgccgatg tcgtagaggt cggggaaggc catggccacg cggtagccga
     1801 cggcgtccca gtccttgacg acctggttca gttcgccgcc ggtgtagcgg ccgggtttgg
     1861 ccacgcgggg gaggaggcgg tcgagagcgg tttcgat
//
LOCUS       DAOSMQ010000575         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_61358_length_1888_cov_2.939989, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000575 DAOSMQ010000000
VERSION     DAOSMQ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61358_length_1888_cov_2.939989"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PK829_14310"
     CDS             complement(<1..543)
                     /locus_tag="PK829_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42437.1"
                     /translation="MNDATRSLIQAIHDSPPRAVFAAAGAGSQALADLLGVPGASRTL
                     LEALIPYAADAFDEFLGGAPEQYVSAETGRRMAGRALARARHLRELEAWPLVGLACTA
                     TIITDRPKKGEHRAHVAAWTPARLRVYALRLDKGARDRAGEEGLVSALLLDALAEASG
                     VAARPALPLGPGDSLTDETVD"
     gene            747..>1888
                     /locus_tag="PK829_14315"
     CDS             747..>1888
                     /locus_tag="PK829_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42438.1"
                     /translation="MKRPLRLPSLLVSMLLMSMLLVLLTACRPGAAAVPTTAPTAVLP
                     ATTVPEATASTAISVAVTPITDDEPPATAAVATAAVATAATNAAPLFDVAWDDRAIYA
                     SGLVDPAAEDVPEAPVYHIAITIANDMHTVDGRQTVFYTNAEDVPLSEIVFHLHPNLL
                     DSAIDVSDMTVDGAPAPTAISGAKRSILTVTLPAPLPPGATTTLDMRFHTEVATDIGR
                     NYGVLAFFDGILALAHFYPMLSVYDATGWNTAEADVQGDLTYSDAGYYLVRVAAPPGV
                     TVVASGVAVEEQALSQGGPSSALREGPGEGETVAVYAVGPARDFYLAAGDFEVVSGTA
                     GATTINSYAPAAWRDGAALALDVAAAAIDINEARLSPFPYTELDIVT"
BASE COUNT          276 a          715 c          615 g          282 t
ORIGIN      
        1 atcgacggtc tcatcggtca ggctgtcgcc cggccccaac ggcagggccg gccgcgccgc
       61 cacgcccgac gcttcggcca gcgcgtccag cagcagcgcg ctgaccagcc cctcctcgcc
      121 cgcccggtcg cgcgcgccct tgtccaggcg cagcgcatag acccgcagcc gcgccggcgt
      181 ccaggcagcc acgtgggcgc ggtgctcgcc cttcttcggc cggtcggtga tgatggtcgc
      241 ggtgcaggcc agccccacca gcggccacgc ctctaactcg cgcaggtgac gcgcccgagc
      301 cagcgctcgc ccggccatgc gccgccccgt ttcggccgag acgtattgtt ccggcgcgcc
      361 gcccaggaac tcgtcaaagg cgtcggcggc gtaggggatg agcgcctcca gcagcgtccg
      421 tgatgcgccg ggcacgccca acaggtcggc cagggcctgg ctgcccgccc cggcggcggc
      481 aaagacggcc cgtggcgggc tgtcgtgaat ggcctggatg aggcttcgcg tggcgtcgtt
      541 catagggcta ttgtaatcgg ctcgcgaggg tggcgctatc ggccgcgccg tgccgggcac
      601 gttcttgatt gcctggcccc ttcctgcgcc ggcgaccaaa gaaggcgcgc tacgctcaag
      661 aaagaacgcc cctccggcag accggtgcat gagtcttgcc gctgtttctt tgcgcggcgg
      721 agagggctgg agacaattgc gcgatcatga aacgacctct gcgcctgccc tcgctcctcg
      781 tgtccatgct tctcatgtcc atgctcctcg tgctcctgac cgcttgccgc cccggcgcgg
      841 cggccgtgcc caccacggcg ccaaccgctg tcctgccggc caccaccgtg cccgaggcca
      901 cggcgtctac agcgatatcg gtagcggtga cgccgatcac tgacgacgaa ccgccggcta
      961 ccgctgcggt ggccacggca gcggtggcca cggcagcgac aaacgccgcg cccctcttcg
     1021 acgtagcctg ggacgatcgc gccatctacg ccagcggcct cgttgacccg gcggccgagg
     1081 acgtacccga agcgcccgtc taccacatcg ccatcaccat cgccaacgac atgcacactg
     1141 tcgatgggcg gcagacggtc ttctacacca acgctgagga tgtccccctg agcgagattg
     1201 tctttcatct gcaccccaat ttgctcgaca gcgccatcga cgtcagcgac atgaccgtcg
     1261 atggcgcgcc cgcccccacg gccatcagcg gggccaagcg cagcatcctg accgtgaccc
     1321 tgcccgcgcc gctgccgccg ggggcgacga cgaccctcga catgcgcttc cacacggagg
     1381 tggccaccga catcggccgc aactatggcg tcctggcctt tttcgatggc atcctggccc
     1441 tggcccactt ctaccccatg ctgtcggtct acgacgccac cggctggaac acggccgagg
     1501 ccgacgtgca aggcgacctg acctacagcg acgccggcta ctacctggtg cgcgtggccg
     1561 cgccaccggg cgtcacggtc gtggcctcgg gcgtggccgt cgaagagcaa gccctctccc
     1621 aggggggccc gtcctccgcc ctgcgggagg ggccagggga gggcgagacg gtagcggtct
     1681 atgccgtcgg cccggcccgc gacttctatt tggcggcggg cgacttcgag gtggtcagcg
     1741 gcacggccgg cgcgacgacg atcaacagct acgccccggc ggcgtggcgc gacggcgcgg
     1801 cgctggctct cgacgtggcc gcggcggcca tcgacatcaa cgaggcgcgc ctgtcgccct
     1861 tcccctacac cgaactcgac atcgtcac
//
LOCUS       DAOSMQ010000576         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_61451_length_1886_cov_3.117968, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000576 DAOSMQ010000000
VERSION     DAOSMQ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61451_length_1886_cov_3.117968"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PK829_14320"
     CDS             <1..470
                     /locus_tag="PK829_14320"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOU42439.1"
                     /translation="SGRRTILVDCDLRRPSLHALFGLAMTPGLTNLVLEELAELPLQP
                     TGVDSLWLLSSGPTPPNPADLLGASRMDRVLAQLSEAADIVLFDAPPVVAAADAAILG
                     AKADGVLLIIQAGRTRRDQSERARELLEQAKARIVGVALTNAPRESGAAEYYG"
     gene            502..1569
                     /locus_tag="PK829_14325"
     CDS             502..1569
                     /locus_tag="PK829_14325"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOU42440.1"
                     /translation="MKGLILSGGTGSRLYPLTYTSAKQLIPVANKPILFRVIETIRDA
                     GIDDIGIVVGNTAEQIKEAVGRGGRWDVRITYIPQEKPLGLAHAVKVAQPFLGDDRFV
                     MFLGDNVIQGGISNLIREFARSDWNSQIVLTRIEHPEQYGVAELGEDGRIIRLVEKPK
                     SPPSDLALVGIYMFDASVFQAVEAIKPSWRGELEITDAIQWLVDNGRTVHPYIHEGWW
                     IDTGAPGDMLSANDLVLEEIAYNIEGYVDRDSNVDHRVTVQRGAEVIGSIIRGPAIIG
                     EYARIVNSYIGPFTSIDHHVTIENSEIEHSMVLENSRICDIETRIQDSLIGRDVVIAR
                     SPIKPKALKLTVGDHSKMGIQ"
     gene            1637..>1886
                     /gene="dtd"
                     /locus_tag="PK829_14330"
     CDS             1637..>1886
                     /gene="dtd"
                     /locus_tag="PK829_14330"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOU42441.1"
                     /translation="MRALIQRVARASVTVDGQTVGRIGRGFVVLLGVTHADGRAEAGW
                     VAAKIAGLRVFDDEAGKMNLSLADVGGELLVVSQFTLYG"
BASE COUNT          334 a          638 c          616 g          298 t
ORIGIN      
        1 agagcggccg ccgcaccatc ctggtcgatt gcgacctgcg ccggccgtcg ctccacgcgc
       61 tgttcggcct ggccatgacg cccggcctga ccaacctggt gctagaggag ctggccgagc
      121 tgccgttgca accgaccggc gtggatagcc tgtggctgct gagcagcggg ccgacgccgc
      181 ccaacccggc cgacttactc ggcgcgagcc gcatggaccg cgtgttggcc caactgtccg
      241 aggcagccga tatcgtcctg ttcgacgcgc cgcccgtggt tgccgcggcc gacgccgcca
      301 tcctgggagc caaggccgac ggcgtgctgc tgatcatcca ggccggccgc acccggcgcg
      361 accagtccga gcgcgcccgc gagctgttgg agcaggccaa ggcgcgcatt gtcggcgtgg
      421 ccctgaccaa cgcgccgcgc gagagtggcg cggcggagta ctacggctaa gcgcgccggc
      481 gcagcgactt gcggaggggc aatgaaagga cttatcctca gcggcggcac aggctcgcgg
      541 ctctacccac tgacctacac cagcgccaag cagctcatcc cggtggccaa caagccgatc
      601 ctctttcgcg tcatcgagac gattcgcgac gcgggcatcg acgacatcgg catcgtcgtc
      661 ggcaacaccg ccgagcagat caaggaggcc gtcggccgcg gcgggcgctg ggacgtgcgc
      721 atcacctaca ttccgcagga gaagccgctg gggctggccc acgccgtcaa ggtggcgcag
      781 ccatttctgg gcgacgaccg tttcgtgatg ttcctgggcg ataatgtcat ccagggcggc
      841 atcagcaacc tcatccgcga gttcgcccgg agcgactgga atagccagat cgtcctgacg
      901 cgcatcgagc acccggagca gtacggcgtg gccgagttgg gcgaggacgg ccgcatcatc
      961 cgtctggttg agaagcccaa gtcgccgccg agcgacctgg ctctggtggg catttacatg
     1021 ttcgatgcca gtgtctttca ggcggtggag gccatcaaac cgtcgtggcg gggcgagttg
     1081 gagatcaccg atgccattca gtggctggtc gacaacggcc gcaccgtcca cccctatatc
     1141 catgagggct ggtggatcga caccggcgcg ccgggcgaca tgctctcggc caatgatctg
     1201 gtgctagagg aaatcgccta caacatcgag ggctacgttg accgcgacag caatgttgac
     1261 caccgcgtca ccgtccagcg gggcgctgag gtcatcggca gcatcatccg cggcccggcc
     1321 atcatcggcg agtatgcccg tatcgtcaac agctacatcg gccccttcac cagcatcgac
     1381 caccacgtca ccatcgagaa cagcgagatc gagcactcga tggtgctgga gaacagccgc
     1441 atctgcgaca tcgaaacgcg catccaggat agcctcatcg gccgcgatgt ggtcattgcc
     1501 cgctcgccga tcaagcccaa agccctgaag ctgaccgtgg gcgaccacag caagatgggc
     1561 atccagtaac cgcaccctgg cggggcggca tgggcttgcg tagtcctgcc cagccgctgc
     1621 gaaagcgaca tctcctatgc gtgccctcat ccagcgcgta gcccgcgcca gtgtaaccgt
     1681 ggacggccag accgtcggcc ggatcggccg cggctttgtc gtcctgctgg gcgtgaccca
     1741 cgccgacggc cgcgccgagg ccggatgggt ggccgccaag atcgccggcc tacgcgtctt
     1801 cgatgacgag gccggcaaga tgaatctatc gctggccgac gtgggcgggg agcttctcgt
     1861 cgtgtcgcag ttcacgctct atggcg
//
LOCUS       DAOSMQ010000577         1875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_61966_length_1875_cov_3.935714, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000577 DAOSMQ010000000
VERSION     DAOSMQ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61966_length_1875_cov_3.935714"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PK829_14335"
     CDS             complement(<1..741)
                     /locus_tag="PK829_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOU42442.1"
                     /translation="MDEPTRDDALTFDEFPIPTADEWRAAAVDSLGGQPFEKLISRTY
                     EDIPLQPLYRREDTAELAYAQTLPGQPPYLRGRTAAGPTQHGWAIAQELTEHDPAIFN
                     AALITALEHGQTAVTIRLDDGAGAGLRLATVADVATALRGVYLQGLPVILHAGPTALP
                     LLALLAAARPAGAAEGGLAELTGSIAADPLAALAETGALPLPLASAYDDMALVAEWAA
                     AAAPGLATVAVRAGLYHEAGGSAVHELAL"
     gene            956..1456
                     /locus_tag="PK829_14340"
     CDS             956..1456
                     /locus_tag="PK829_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOU42443.1"
                     /translation="MAIKQGWHGRLFEDFEVGDVYPHPLGRTVTQNDNIWFSLLTMNT
                     NPIHFDSAYSAQTEWGTPLVNSAFTLSLVMGMSVGDISQNAVNLGWDEIRLPNPLYEG
                     DTVYAQSEVLEKRESNSRPHQGIVTIRTTGMNQDGLPIITFKRTILVYKRGYAPAAHR
                     PTLQTS"
     gene            1510..>1875
                     /locus_tag="PK829_14345"
     CDS             1510..>1875
                     /locus_tag="PK829_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOU42444.1"
                     /translation="MEPQMEPLNVTIRELPPVQVAALEYNAEGLAGTYSESIGMLFRE
                     VEGWLRLRGYDTDALRRIGVPFVEGEMLRRYWCCIEAPAGVTTDDGPVMLRQLAGGRY
                     AVLTLPKDSATIGESIGRFH"
BASE COUNT          302 a          601 c          679 g          293 t
ORIGIN      
        1 gagggccagt tcgtggacgg cgctgccgcc ggcttcatgg taaaggccgg cgcggacggc
       61 gaccgtggcc agccccggcg cggccgcggc ggcccactcg gccacaaggg ccatatcatc
      121 gtaggccgac gccagcggca acggtagcgc ccccgtctcg gccagcgccg ccagcgggtc
      181 ggcggcgatg ctgccggtca gttcggccaa gccgccctcg gccgcgcccg cgggccgcgc
      241 cgcggccagc agcgccagca gcggcagcgc cgtcggcccg gcgtggagga tcaccggcag
      301 gccctggagg tagacgccgc gcagggccgt ggcgacgtcg gccacggtgg ccagccgcag
      361 gcccgcgccc gcgccgtcgt cgaggcggat ggtgacggcc gtctgcccgt gctccagcgc
      421 cgtgatgagg gcggcgttga agattgccgg gtcgtgctcg gttagctcct gggcgatggc
      481 ccagccgtgt tgcgtcgggc cggcagctgt ccggccgcgg aggtagggcg gttgccccgg
      541 cagcgtctgg gcataggcca actcggccgt gtcctcgcgc cggtagagcg gctgcaaggg
      601 gatatcctcg taggtgcgcg aaatcagctt ctcgaacggt tgcccgccga gcgagtcgac
      661 ggccgcggcc cgccattcgt cggcggtggg gatggggaac tcgtcaaaag taagggcgtc
      721 gtcccgtgtt ggttcgtcca tgtttctgta ttcaccggag cgccagagcg tggccggcgc
      781 gggttggttg ggcgcgaacc ggccacagtg gtcgatccgg tcttgagccg gattgtatag
      841 tactccagcc gcgtccgcat gtcaggtgat gtgtgtcacg tcggaggcag gcaaaagtca
      901 tatgtccccg cgccgcgcag cggttataat cccgacgacc tatgaggagg tatctatggc
      961 catcaaacaa ggctggcacg ggcggttgtt cgaggatttc gaggtcggcg acgtctatcc
     1021 ccacccgctc ggccgcaccg tgacgcaaaa cgacaacatc tggttctcgc tgctgacgat
     1081 gaacaccaac cccatccact tcgatagcgc ctactcggcg caaacggagt ggggcacgcc
     1141 gctggtcaac tcggccttca ccctgtcgct ggtgatgggc atgtcggtcg gcgacatctc
     1201 gcagaacgcg gtcaatctcg gctgggacga gattcggttg cccaacccgc tctacgaggg
     1261 cgataccgtc tatgcccaga gcgaggtgct ggagaagcgc gagtcgaaca gccggcccca
     1321 ccagggcatc gtcaccatcc gcacgacggg gatgaatcag gacgggctgc cgatcatcac
     1381 cttcaagcgg acgatcctgg tctacaaacg cggttatgcg ccggcggccc accggccgac
     1441 gctacagacg agctaggccg tccacgcggc gcgggctaag cgcggggtgg ggggccagga
     1501 gatagacgca tggaaccgca gatggaaccg ttgaacgtga cgattcggga gttgccgccg
     1561 gtgcaagtcg cggcgctgga gtacaacgcc gaggggctgg ccgggacgta tagcgagagc
     1621 atcggcatgc tcttccgcga ggtggagggc tggctgcggc tgcgcggcta tgacaccgac
     1681 gcgctgcggc gcataggcgt gcccttcgtc gagggcgaga tgctacggcg ctactggtgt
     1741 tgcatcgagg ccccggctgg agtgacgacc gacgacggcc cggtgatgct gcgccagttg
     1801 gccggcgggc gctatgccgt gctgacgctg cccaaggatt cggccaccat tggcgagagc
     1861 atcggccgct tccac
//
LOCUS       DAOSMQ010000578         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_62714_length_1858_cov_4.074321, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000578 DAOSMQ010000000
VERSION     DAOSMQ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62714_length_1858_cov_4.074321"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PK829_14350"
     CDS             complement(<1..732)
                     /locus_tag="PK829_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42445.1"
                     /translation="MRKRDLPPLVLGILITAVAACRPTPTLPNANSPAALEPEWAEPA
                     VDPATIAPATASIPLAASPPVFASSPIAPTAEVAFESGQLLGSDRAGLSLRIPSDWID
                     LTAQSNIPAMGNRLGINLVFAADSERTGRSLLAGKAFARGAYVSGLIVTPNAPTADPT
                     AALADLLRAAAPTAVPLTAPAPVVSANGVAGVALEVSDGPIGVNASATDLRTRVVLYL
                     PEADSGYSSWVVLLLSASTPRWPAAA"
     gene            complement(745..>1858)
                     /locus_tag="PK829_14355"
     CDS             complement(745..>1858)
                     /locus_tag="PK829_14355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOU42446.1"
                     /translation="VDLRCDEEEGAPTHSDTIIVATIDPLSQSAALLSLPRDLWVEVP
                     NYGANRINQAYFFGQAYEYPGGGPQLAAETVEAFLGVPIDYYVAVDFQAVIDFIDLMG
                     GVVVEVPERIEDPTYPDSCYGYDPFTIDPGQQRLDGATALKYARTRATFGGDVDRAGR
                     QQQVINAVREQATELDTLPQLLLRAPQLWQSYQEHVTTNLSFDEALQLANFVRTMPRE
                     NIRNVVLDYSYVYNDTTFDGQQVLVPVREKVRVLRDEVFAPPVVPTPVLEALPTAITV
                     EDARVAVFNGTPTFGLASDTQAYLLSRDVNVTEIGNADSATYSATQIIDYGSHPGTVQ
                     YLVQLMNVPPLNVSTGVNPAGEFDVLVILGSDWQVP"
BASE COUNT          308 a          552 c          695 g          303 t
ORIGIN      
        1 cgcggccgcc ggccagcgcg gcgtcgaggc gctaagcagc aggacgaccc aggacgagta
       61 gccgctatcg gcctctggca agtagaggac gacgcgcgtg cgcaggtctg tggccgacgc
      121 attgacacca atcggcccgt cgctcacctc cagagccacg ccggccacgc cgttggcaga
      181 gacgacgggc gcgggcgcgg tcaggggcac ggccgtcggc gcggcggcgc gcagcagatc
      241 ggccagggcc gcggttgggt cggccgttgg cgcgttgggc gtaacgatca ggccggagac
      301 gtaggctccg cgcgcgaacg ctttgcccgc caacaggctg cgccccgttc gctccgagtc
      361 agcggcgaag acgaggttga tgcccagccg gttgcccatg gccgggatgt tgctctgggc
      421 ggtcaggtcg atccagtcgc tggggatgcg cagggacaga ccggcgcggt cgctgccgag
      481 cagttggccg gactcgaacg cgacctcggc tgttggggcg atgggcgaac tggcgaagac
      541 cggcggcgag gcggctaagg ggatagaggc ggttgccggc gcaatggtgg cgggatcgac
      601 cgccggttca gcccactctg gctccagcgc ggccgggcta ttggcgttgg gcagggtggg
      661 tgttgggcga caggctgcca ccgcggtgat caggataccc aggaccaggg gcggcagatc
      721 gcgcttccgc atggctccgg ccggctaggg aacctgccag tcgctcccca gaatgacgag
      781 aacatcaaac tcgcctgctg ggttgacgcc cgtgctgacg ttcagcggcg ggacgttcat
      841 aagctggaca aggtattgaa ctgtgccggg atgcgagccg tagtcgatga tttgggtggc
      901 cgagtaggtg gccgagtcag cgttgccgat ctcggtcaca ttgacatcgc ggctgagcag
      961 gtaggcctgc gtgtcggaag ccaggccgaa ggtcggcgtg ccgttgaaga cagccacgcg
     1021 cgcatcttcg acggtgatgg ccgtgggcag cgcttccagg acaggcgtgg gcacgaccgg
     1081 tggcgcaaag acctcatcgc gcaggacgcg caccttctcg cgcacgggca cgagcacttg
     1141 ctggccgtca aaggtggtat cgttgtagac gtagctgtaa tcgagaacga cgttgcggat
     1201 gttctcgcgc ggcatggtac gcacgaaatt ggccagttgc agcgcctcgt caaaggacag
     1261 gttggtggtc acgtgctcct ggtacgattg ccacagttgg ggcgcgcgca agagcagctg
     1321 gggcagggtg tccaattcgg tggcctgctc gcggacggcg ttgatgacct gttgctgccg
     1381 gccggcgcga tcgacatcgc cgccaaaggt cgcgcgggtg cgcgcatact tcagcgccgt
     1441 cgcgccatcg aggcgctgct ggccgggatc gatggtgaag gggtcatagc cgtagcaact
     1501 atcggggtag gtcgggtctt cgatgcgctc cggcacttcg acgacgacgc cacccatcag
     1561 gtcgatgaag tcgatgacgg cctggaagtc gacggccaca tagtagtcga tgggcacgcc
     1621 cagaaaggcc tcgaccgtct ccgcggccag ttgtggcccg ccgccggggt attcgtaggc
     1681 ctggccgaag aagtaggcct ggttgatgcg gttggccccg tagttgggaa cctccaccca
     1741 caggtcgcgt ggcagcgaca gcagcgccgc cgactggctg agcgggtcga tggtggcgac
     1801 gatgatggtg tcgctgtggg tgggcgcgcc ctcctcttcg tcgcaacgca gatcgacg
//
LOCUS       DAOSMQ010000579         1852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_63018_length_1852_cov_3.589872, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000579 DAOSMQ010000000
VERSION     DAOSMQ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63018_length_1852_cov_3.589872"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PK829_14360"
     CDS             <1..225
                     /locus_tag="PK829_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42447.1"
                     /translation="GESLFEPLTVMAARPAAAEEVVAPAAAQPAAVAAPAAESDAGPA
                     GALRWAALALVLVAVALLVVQSRRPAAVKS"
     gene            228..662
                     /locus_tag="PK829_14365"
     CDS             228..662
                     /locus_tag="PK829_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOU42448.1"
                     /translation="MAHIANKRVTAGLLVVALALAALSFVDWGKAGAAPPATTAASPP
                     ATPSPTPAEVGRALFQAKGCTSCHRHDGVGIARVAGSAADAPALAGLGAPDLTHYQPD
                     PDFVRRWLHDPRAVRPDTAMPNLRLSDTDIEALLAFLQTNQP"
     gene            complement(695..1111)
                     /locus_tag="PK829_14370"
     CDS             complement(695..1111)
                     /locus_tag="PK829_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU42449.1"
                     /translation="MQATRIFETVIYANDLAAAERFYGEVLGLKVYSRSDTVVAFHCG
                     DGVLLVFDPDRAILPNRGVPSHGAHGPGHLAFAMRAGEADGWRAQLAAHGVAIEMEID
                     RHPDGLSLYFRDPAGNSIELAPPTLWGCGRGDAAQT"
     gene            complement(1281..1745)
                     /locus_tag="PK829_14375"
     CDS             complement(1281..1745)
                     /locus_tag="PK829_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42450.1"
                     /translation="MSLAKRNASNHLTAGCFLGALAAAGGVTVILGVLGVGVTLFVAA
                     PTANWIGLGFALVQSVTFIILAGLFGAAAGLVIAIVDHLVDGRIHDGRMPFWAWLPIG
                     GAMGAATSVLLLIFPLDAKDSTTSLAFYGIMGAVCGLVAGPVFGLCFRQRRA"
BASE COUNT          317 a          664 c          602 g          269 t
ORIGIN      
        1 ggcgagtcgc tttttgagcc gctgaccgtg atggcggcgc ggcccgccgc ggccgaggaa
       61 gtcgtcgccc cagccgccgc ccaaccggct gcggtggccg cgccagccgc cgagagcgac
      121 gccgggccgg cgggcgcgct gcgctgggcg gcgctggccc tggtgctggt cgccgtggct
      181 ttgctggttg tccagagccg gcggcccgcg gcggtgaagt cgtaggcatg gcccacatcg
      241 ccaacaagcg cgtcacggcg gggctgttgg ttgtggccct ggcgctggcg gcgctatcct
      301 ttgtggactg gggtaaggcg ggcgcggctc cgccggcaac gaccgccgct tcgcccccgg
      361 caacgcccag cccgacgccg gctgaagtgg gtcgggcgct gttccaggcc aagggctgca
      421 cgtcgtgtca ccgccatgac ggcgtcggca ttgcgcgcgt cgccggcagc gcggccgacg
      481 ccccggccct ggccggcctg ggcgcgcccg acctgaccca ctaccagccc gaccccgact
      541 ttgtccgtcg ctggctgcac gacccgcggg ccgtccgccc cgacacagcc atgcccaacc
      601 tgcgtctgag cgacaccgac atcgaggcgc tactggcctt tttgcaaacg aaccagccct
      661 agaggaccga cctgacaggt gtgcagggca cctgtcaggt ctgggccgcg tcaccccgcc
      721 cgcaacccca caacgtcggc ggggccaact cgatgctgtt gccggccggg tcgcggaagt
      781 agagcgacag cccgtcggga tggcggtcga tctccatctc gatggccacg ccgtgggcgg
      841 ccagttgcgc ccgccagccg tcggcctcgc cggcgcgcat ggcaaaggcc agatggccgg
      901 ggccatgcgc gccgtggctg ggcacgccgc ggttgggcag gatcgcccgg tcgggatcga
      961 agacgagcaa cacgccatcg ccacagtgga aggccacaac ggtgtccgaa cggctgtaga
     1021 ccttcaatcc cagcacttcg ccgtagaagc gctcggccgc ggccagatca ttggcgtaga
     1081 tgaccgtctc gaagatgcgt gtggcctgca tggtcaatcc tccagtcgct gtcttgttgt
     1141 agcgcggcat accatcccgc ggttccacca ccccagattg tagcccggga tgccatcccg
     1201 cggttccagc gcgagatacc atcccgcgct acaaggttga acccgattga acagtcatga
     1261 aagcgggttg cgtttaggct tcacgcgcgt cgctggcgaa agcacagccc aaagaccggc
     1321 ccggccacca gcccacacac cgcgcccatg attccgtaga aggccagaga cgttgtcgag
     1381 tctttggcgt cgagcgggaa gatgaggagg agaacagacg tcgccgcgcc catagcccca
     1441 ccgatcggca gccaggccca gaagggcatg cgaccgtcgt gtatccgacc gtcaaccaga
     1501 tgatcgacga tggcgatcac aaggccggcg gccgccccaa aaagtccggc caggatgatg
     1561 aacgtgacgc tttgcacaag ggcgaagcca agccctatcc agttcgcagt gggcgcggcg
     1621 acaaacagag tcacgcccac gcccaacaca ccgaggatca cagtcacgcc gccggccgcg
     1681 gccagcgccc ccaagaaaca gcccgccgtc aggtgattac tcgcgtttcg cttcgccagc
     1741 gacatcgctt tctcctgtct acgaacgatt cagccactgc cccgacacca tgctcagccc
     1801 caccccctgc cgccggtagc ccagcttgcg atagaagccc tcggctgagt cg
//
LOCUS       DAOSMQ010000580         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_63464_length_1843_cov_4.901566, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000580 DAOSMQ010000000
VERSION     DAOSMQ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63464_length_1843_cov_4.901566"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PK829_14380"
     CDS             <1..267
                     /locus_tag="PK829_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOU42451.1"
                     /translation="AIAHLIATGQYGTYHFVNSGACSRWAFANEILRQAGVAATNVPI
                     LSSAYPRASTPPPFGALHNRAGAALGITLRPWPEALADYLRDAL"
     gene            264..1247
                     /locus_tag="PK829_14385"
     CDS             264..1247
                     /locus_tag="PK829_14385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU42452.1"
                     /translation="MNTPSSPRVSVIIPHWNGRQHLDDCLHALRRQTYADHEVILVDN
                     GSTDGSQAYVRERFPEVRLVELGHNSGFTGAGNAGYAASRGEFICLLNNDTEADAGWL
                     AAIVAVFDSHPRVGVVASKMLLFDRRDYFHTAGDFYRVDGIPGNRGVWQEDRGQYDRE
                     EPVFSACGGAAAYRREMLAEIGFLDNAFFFSCEDIDLAWRAHLAGWDVRYTPAAVVYH
                     KLKASGGSGVVGSYHDGRNFLYVIWKNYPTSLLRQHAGDILRAQLRISGEALRAWRGA
                     AARARLRGQLAGLLALPRLWPARRHIQAARRIDDSTLAARLTPIDATRPDG"
     gene            1341..>1843
                     /locus_tag="PK829_14390"
     CDS             1341..>1843
                     /locus_tag="PK829_14390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42453.1"
                     /translation="MPAYNEAERLPTTLPAVIAFLDAQPYASELILVNNNSRDDTRRI
                     ALDFAATRPYIRVLDEMTQGKGAAVRAGMLAAEGEYLFMADADFSMPVAEIAKFLSPQ
                     LGRYDVAIGSRETPGAMRYNEPVYRHFMGRVFNFYVKVLAIPGFEDTQCGFKCFRREV
                     AHDILPR"
BASE COUNT          297 a          666 c          565 g          315 t
ORIGIN      
        1 gccatcgcac acctgatcgc caccgggcaa tacggcacgt atcactttgt caacagcggc
       61 gcgtgctcgc gctgggcctt tgccaacgag attctgcgcc aggcgggcgt ggccgcgaca
      121 aacgtgccca tcctcagcag cgcctacccg cgggcctcca caccgccccc gttcggcgcg
      181 ctgcacaacc gcgccggcgc ggctctgggc attacgctca ggccgtggcc ggaagcgttg
      241 gccgactacc tgcgcgacgc cttgtgaaca cgccctcctc gccccgcgtc tcggtcatca
      301 tcccgcactg gaacggtcgc cagcacctcg acgactgcct ccatgctctg cgccgtcaga
      361 cctacgccga ccacgaggtc atcctggtgg acaatggctc taccgatggc tcgcaggcct
      421 atgtgcgcga gcgcttcccg gaggtgcggc tggtggagtt gggccacaac agcggtttca
      481 ccggcgcggg caatgccggc tatgccgcca gccggggcga gttcatctgc ttgctgaaca
      541 acgacaccga ggccgacgcg ggttggctgg cggccatcgt cgctgtcttc gacagtcatc
      601 cgcgcgtcgg tgtcgtcgcc agcaaaatgc tgctcttcga ccggcgcgac tacttccaca
      661 ccgccggcga cttctaccgc gtcgatggca tccccggcaa ccgcggcgtc tggcaagagg
      721 atcgcggcca gtatgatcgc gaggagccgg tcttcagcgc ctgcggcggc gcggcggcct
      781 atcgacgaga gatgttggcc gagatcggct tcctggacaa cgccttcttc ttctcctgcg
      841 aagacatcga cctggcctgg cgggctcatc tggccggctg ggacgtgcgc tatacgccgg
      901 cggctgtcgt ctaccacaaa ctcaaggcct ccggcggcag cggcgtcgtc ggcagctacc
      961 acgacggccg taactttctt tacgtcatct ggaagaacta ccccaccagc ctgctgcgcc
     1021 aacatgctgg cgatattctg cgcgcccagc tacgcatcag cggcgaggct ctgcgcgcct
     1081 ggcgcggggc ggcggcgcgg gcgcgcctgc gcggacagct ggccggcctg ctggccctgc
     1141 cgcgcctgtg gccagcccgt cgccacatcc aggcagcgcg ccgcattgac gacagcaccc
     1201 tggccgcccg cttgacgccg attgacgcca cccggccgga tgggtaaact tgacctttgc
     1261 gccgggaaac agcttcgtcc gtcgtccttg cccggcctgt gagccaaatg agcgcccaac
     1321 ctccgcttct gagcctagtc gtgcctgcct acaacgaggc agagcgcctg ccgacaacgc
     1381 tgccggcagt catcgccttc ctggatgccc agccctacgc gtcggaactg atcctcgtca
     1441 acaacaacag ccgcgacgac acgcgccgaa ttgccctgga cttcgccgcg acgcgcccct
     1501 acattcgcgt gctggacgag atgacccagg gcaaaggcgc ggccgtgcgc gcgggcatgc
     1561 tggccgccga gggcgagtat ctcttcatgg ccgacgccga cttttccatg cccgtggcgg
     1621 agatcgccaa gtttctgtcg ccccaactgg gccggtatga tgtcgccatc ggcagccgcg
     1681 aaacgccggg cgcgatgcgc tacaacgagc cagtgtaccg ccactttatg ggccgcgtat
     1741 tcaacttcta cgtcaaggtg ctggccattc ccggctttga agacacacag tgcggcttca
     1801 agtgctttcg ccgcgaagtg gcccacgaca tcctgccccg gca
//
LOCUS       DAOSMQ010000581         1842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_63529_length_1842_cov_3.807499, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000581 DAOSMQ010000000
VERSION     DAOSMQ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63529_length_1842_cov_3.807499"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PK829_14395"
     CDS             complement(<1..1033)
                     /locus_tag="PK829_14395"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOU42454.1"
                     /translation="MFIVDAHLDLAYNALNKGRDPRRKLTEIRAAEAPDYARGIPTVS
                     FSAMRRGGVGLAFATLFNSPQDNPFPDGSEEDECYSTAEEAHRLGMKQVDFYRRLVDE
                     DETLRLVTDQATLDEVVASHDQSHMATGENAQPLLGLLLLMEGADHIRVPDETEVWRD
                     AGVRIIGPAWDDTRYCDGAWRGSKTGLSADGRRLLEVMADLGLILDLTHMSEIAALQS
                     LDAYAGPVVATHANARALVPTERQLSDKQIRLIGERDGVIGCVLFNAFLRAGYRKGDN
                     KATVTLDHVVAHIDHICQLIGDARHAGIGSDLDGGFGAADIPTPLDSIADLRLIGEAL
                     GEKGYSPDDV"
     gene            complement(1077..>1842)
                     /locus_tag="PK829_14400"
     CDS             complement(1077..>1842)
                     /locus_tag="PK829_14400"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOU42455.1"
                     /translation="AKALTDDDISRYRAAVNEYADLFPTCVHASYLINVASPDPALWE
                     RSYAALKEEVERAAALGIDLVTFHPGSYMDSNEAAGLDAIARALRRLLAETAATAPRT
                     IICLETMAGQGTNLGGRFEHLAAIIDRAGGSERLGVCFDTCHVFAAGYDIRTPEAYAA
                     TLAEFDRVVGLGRIQCFHFNDSKHGLGEGKDRHEHIGRGQIGLAGFANFINDPRWANH
                     CAHLETPPTEDADDGTVTDMNQVNLAALRALRPMTE"
BASE COUNT          269 a          595 c          645 g          333 t
ORIGIN      
        1 ccacgtcgtc cgggctgtag cccttttcgc ccagcgcttc gccgatgagg cgcaagtcgg
       61 cgatgctgtc cagcggcgtg gggatgtcgg ccgcgccaaa tccgccgtcc aggtcgctgc
      121 caatgccggc gtgacgcgcg tcaccgatga gctggcagat gtggtcgatg tgggcgacaa
      181 cgtggtcgag cgtcaccgtg gccttgttgt cccccttgcg atagccggcg cgcaggaagg
      241 cattgaatag cacgcagccg atgaccccgt cgcgctcgcc gatgaggcga atctgtttat
      301 cgctcaactg tcgctccgtc ggcacgaggg cgcgggcatt ggcatgggtg gcgacgaccg
      361 ggccggcgta ggcgtcgagg gattggaggg cggcgatctc gctcatgtgg gtcaggtcga
      421 ggatcaaccc caggtcggcc atgacctcca gcaggcgacg gccgtcagcc gaaaggccgg
      481 tcttgctgcc gcgccaggcc ccatcgcaat agcgcgtatc gtcccaggcc gggccaatga
      541 tgcgcacgcc ggcgtcgcgc cacacctctg tttcatccgg cacgcggatg tggtcggccc
      601 cctccatcag tagtagcagg cccagcagcg gttgggcgtt ttcgcctgtg gccatgtggc
      661 tttgatcgtg gctggccacg acctcatcca gcgtcgcctg gtcggtcacc agccgcaatg
      721 tctcgtcttc atcgaccagc cgccggtaga agtcgacctg cttcatgccc aggcggtggg
      781 cttcctcggc ggtgctgtag cactcatcct cctcgctgcc gtcggggaag ggattgtcct
      841 gcggcgagtt gaagagcgtg gcgaaggcca ggcccacgcc gccgcggcgc atagccgaga
      901 agcttaccgt ggggatgcca cgggcgtaat cgggcgcttc ggcagcgcga atttccgtca
      961 gcttgcggcg cgggtcgcgc cccttgttga gggcgttgta ggccaggtcg aggtgagcat
     1021 cgacaatgaa catagagcct ccagatgcgt tagttagggg cgggaacgcg gcgctcttac
     1081 tccgtcatcg gccgcagcgc gcgcagggcg gccagattga cctggttcat gtctgtcact
     1141 gtgccgtcgt cggcgtcctc ggtcggcggc gtctccaggt gggcgcaatg gttggcccaa
     1201 cgcgggtcgt tgatgaagtt ggcgaagccc gccaggccga tctggccgcg cccgatgtgc
     1261 tcgtggcggt ctttgccctc gcccaggccg tgtttgctgt cgttgaagtg gaagcactgg
     1321 atgcgcccca gccccacgac acggtcgaac tcagccagcg tggccgcgta ggcttccggc
     1381 gtgcggatgt cgtagccggc ggcgaagacg tggcaggtgt cgaagcagac gcccagccgc
     1441 tcgctgccgc cggcccggtc gatgatcgcc gccaggtgct cgaagcgccc gccgaggttc
     1501 gtcccctggc cggccatcgt ctccagacag atgatcgttc tgggggcggt cgcggccgtc
     1561 tcggccagca ggcgacgcag cgcccgggca atggcgtcta gcccggccgc ctcgttactg
     1621 tccatatagg agccggggtg gaaggtcacc aggtcgatcc ccagcgccgc cgctcgctca
     1681 acctcttcct tcaacgccgc atacgaccgc tcccacagcg ccgggtcggg cgaggcgacg
     1741 ttgatcaggt agctggcgtg gacgcacgtc gggaagaggt cggcgtactc gttgacggca
     1801 gcgcggtagc ggctgatgtc gtcgtcggtc agggctttgg cc
//
LOCUS       DAOSMQ010000582         1838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_63720_length_1838_cov_3.553561, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000582 DAOSMQ010000000
VERSION     DAOSMQ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63720_length_1838_cov_3.553561"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1838)
                     /locus_tag="PK829_14405"
     CDS             complement(<1..>1838)
                     /locus_tag="PK829_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOU42456.1"
                     /translation="WFLGWQIEQRFGREQALEWLLNTTYYGHLAYGVEAAARLTFDVG
                     AAELTPAEAALLAAIGRDPAANPFDDPAAARAGAASVLATMAARGWADAADSSTDALA
                     RLAPPPGSDSIAPAFARLTRAELERLLGPARLVGGGYQVETTLDLPLQAAAACVAAAG
                     QGEPVGGGPPCPAAAALTDEPSAEAAAVVILDVPTGAIVALAGDAPLTARPVGTLARP
                     FIALSALSQGYTAASLTYDVEQIYLQDGQPYVPPDDGDDRGPLRLREALAAGRAAPAT
                     QMLGWVGVARVLENAGALGVMASDATSGLAFAQEGFSADLLSLSHAFAALGNGGTLAG
                     APGAERPRPATIRRVLDVQGSEVYALGPETRDVVSPELAWLLTDMLRADAGGAALATG
                     RAAAGDDWAVGLSPERLVGVWAGGAAGDDNAAAPIWQALFDQALGGQPAGEWPRPVGL
                     HAVDVCALSGLLPSRRGPACPTVIEWFVAGTEPTATDTMTREVAVNRETGRLATVFTP
                     PNLVERRVFTVFPPEAAQWATEHDILAPPTEYDPIRRVPTRSGGAAVTSPEPWAVVGR
                     QDSPQGQWPVVGSAGGDDFAYYRLSWFEGLMPEAMQLIVARGETAV"
BASE COUNT          242 a          712 c          661 g          223 t
ORIGIN      
        1 ccacggccgt ctccccgcgc gcgacgatga gctgcatcgc ctccggcatc agcccctcga
       61 accatgacag ccggtagtag gcgaaatcat cgccgccggc gctaccgacc accggccact
      121 gaccttgagg cgagtcctgt cgcccgacca ccgcccacgg ctccggtgac gtcaccgccg
      181 cgccgcccga ccgcgtgggc acgcggcgga tgggatcgta ctcggtcggt ggggcaagga
      241 tatcatgctc ggtcgcccac tgggccgctt cgggtgggaa gacggtgaag acgcggcgct
      301 cgaccaggtt cggcggggtg aagacggtcg ccaaccggcc cgtctcgcgg ttgacggcta
      361 cctcgcgggt catcgtgtcc gtggctgttg gctccgtgcc ggccacaaac cactcgatca
      421 ccgtcgggca agccggcccg cggcgcgacg gcagcagccc ggacagggca cagacatcga
      481 cggcatgcag ccccaccggc cgcggccact cgccggccgg ctgtccgccc agcgcctggt
      541 cgaacagcgc ttgccagatg ggcgcggcgg cattgtcatc cccggccgcg ccgccggccc
      601 agacgcccac cagccgctcg ggcgacagcc ccacggccca gtcgtcgccg gcggccgcgc
      661 gccccgtggc cagagccgcg ccgccggcgt cggcgcgcag catatcggtc agcagccagg
      721 ccaactccgg actcactacg tcgcgcgttt ccgggccgag ggcatagacc tcacttccct
      781 gcacatccag cacacgcctg atcgtcgccg ggcgtggccg ctccgcgccg ggcgcgccgg
      841 ccagcgtgcc gccgttgccg agggcggcga aggcgtggct caggctgagc aagtcggccg
      901 agaagccctc ctgggcgaag gccaggccgg acgtggcgtc gctggccatc acgcccagcg
      961 cgccggcgtt ctccagcacc cgcgccacgc ccacccagcc gagcatctgt gttgccgggg
     1021 cggcgcggcc ggcggccagc gcctcgcgca gccgcagcgg cccacggtcg tcgccgtcgt
     1081 cgggcggcac gtagggctgg ccgtcctgta gatagatctg ctccacgtcg taggtcaggc
     1141 tggcggcggt gtagccctgg ctgagcgccg acagggcgat gaacggccgc gccagcgtgc
     1201 ccaccggccg ggcggtcagc ggcgcgtcgc cggccagggc tacgatcgcc ccggtgggta
     1261 cgtccagaat gacgacggcc gccgcctcag ccgagggctc atccgtcagc gccgcggccg
     1321 ccgggcaggg cggcccgccg ccgaccggct cgccctggcc ggccgcggcc acacaggccg
     1381 ccgcggcttg cagcggcagg tctagcgtcg tctccacctg gtagccgccg cccaccagcc
     1441 gcgccggccc cagcaaccgc tccaactccg cgcgggtcag ccgggcaaag gccggggcga
     1501 tggagtcgct gcccggtggc ggggccaggc gggccagtgc gtcggtggaa ctgtcggctg
     1561 cgtccgccca gccgcgcgcg gccattgtcg ccagcacgct ggccgcgccc gcccgcgccg
     1621 ccgcggggtc gtcgaacggg ttggcggccg ggtcgcggcc gatggcggcc aggagcgccg
     1681 cctcggccgg cgtcagttcg gccgcgccaa cgtcgaaagt gagccgcgcc gctgcctcga
     1741 cgccgtaggc caggtggccg tagtaggtcg tgttgagcag ccattccagc gcttgctcgc
     1801 ggccgaagcg ctgttcgatt tgccagccca gaaaccag
//
LOCUS       DAOSMQ010000583         1831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_64066_length_1831_cov_3.471847, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000583 DAOSMQ010000000
VERSION     DAOSMQ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64066_length_1831_cov_3.471847"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PK829_14410"
     CDS             complement(<1..894)
                     /locus_tag="PK829_14410"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HOU42457.1"
                     /translation="MKANTVRLLPLLLFFTVALPACGSFGPTDSRADEPVVFEPTGAA
                     PTDEPADAPITEDAGGYARAFYRAWELGDYLGMYSLLAPSSQAVVDSAAFVARYAETM
                     DTAAIDAVHTQPLAMLQEGDTAEYGVRVTLTSAVVGDIVRDHTMRLAYEGGRWGVVWD
                     EGLILPELAGGNRLALELRIPSRGNIYDRDGQALAYQGTAYELGVVPGQITDEPGLLA
                     ALSATLGTPPDDIKAKYATAMPDWYVPVGVVSEETLQANALALEPFLEKGLTTPDRRP
                     SRLYSDTNSAAHVIGYLGQIPA"
     gene            1026..1289
                     /locus_tag="PK829_14415"
     CDS             1026..1289
                     /locus_tag="PK829_14415"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOU42458.1"
                     /translation="MEMTKLTIRVPIAVLERAKKYAENNQTSVTRLISQYLSQLPVEN
                     DYLQDAPIVQSLIGILSPETTIEDYWRYIEEKYGDGLSNPDRS"
     gene            1261..1683
                     /locus_tag="PK829_14420"
     CDS             1261..1683
                     /locus_tag="PK829_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOU42459.1"
                     /translation="MAYRILIDLNVVVDVLYKREPFFETSAAVLAAAEARHIEGWLAA
                     HSVTTLFYLIARNTSPAAARVHLTRLVQFLKIAAVDGRAIEQALALPYRDFEDAVQMM
                     AGVHAGVEHVVTRDLSGFKAGPLPTLTPPELLALLRRS"
     gene            1683..>1831
                     /locus_tag="PK829_14425"
     CDS             1683..>1831
                     /locus_tag="PK829_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HOU42460.1"
                     /translation="MDWKLEVVIVPVSDIDRARHFYETQVGFVVDHDTALSEDVRIVQ
                     LTPPGS"
BASE COUNT          352 a          542 c          599 g          338 t
ORIGIN      
        1 ggccggaatc tggcccaggt agccgatgac gtgggcggcc gagttcgtgt cggagtagag
       61 gcgcgacggc cggcggtcgg gtgtggtcag ccccttttcc aggaacggct ccagcgccag
      121 ggcattggcc tgcaacgtct cctcgctgac cacgccgacg ggcacgtacc agtcgggcat
      181 ggccgtggcg tacttggcct taatatcgtc ggggggcgtg cccagcgtcg cgctgagcgc
      241 ggccagcagc cccggctcgt cggtgatctg ccccggcacg acgcccagct cgtaggccgt
      301 gccctggtag gccagcgcct ggccgtcgcg gtcgtagatg ttgccacgcg agggaatgcg
      361 tagctccagc gccagccggt tgccgccggc cagctccggc aagatcaacc cctcgtccca
      421 gacgacaccc cagcgcccgc cctcgtaggc caggcgcatg gtgtggtcgc gaacgatgtc
      481 gccgacgacg gccgaggtca acgtgacgcg gacgccatac tcggccgtgt cgccctcttg
      541 cagcatggcc agcggttggg tatggacggc gtcgatggcc gccgtgtcca tcgtctcggc
      601 atagcgagcc acgaaggcgg cgctatcgac caccgcctgg ctgctggggg ccagcaggct
      661 gtacatgccc agatagtcgc ccagctccca ggcgcgatag aaggcgcgcg cgtagccgcc
      721 ggcgtcctcg gtgatgggcg cgtcggccgg ctcatccgtc ggcgccgcgc cggtcggctc
      781 aaagacaacc ggctcgtcag cgcgcgagtc ggtggggccg aaggagccgc aggcagggag
      841 ggcgacagta aagaagagaa gcagaggtaa cagacggacg gtgtttgctt tcatgagcgt
      901 ctttccaagt agcattccgg ctcattatag cgacttaaga gagacataga gtatgcagtt
      961 aggcaatagt taaggttgaa agccgtacag tttcgtgtta agatgtacgg cacggaggtg
     1021 ggcgtatgga aatgacgaag ttaacaattc gcgtgccgat cgctgtgctt gaacgtgcca
     1081 agaagtatgc agagaataat cagacctcag tcacacgact catatcgcaa tacctaagtc
     1141 agttgccagt tgagaacgac tacctgcaag atgcgcctat tgtccagagc ctcataggca
     1201 ttctatcgcc agagacgacc atagaggatt attggcgcta cattgaagag aagtatggcg
     1261 atggcctatc gaatcctgat cgatcttaac gtcgttgtgg acgtgcttta taagcgcgaa
     1321 cccttctttg agacgtcggc ggctgtccta gccgcggccg aggcaaggca catcgagggt
     1381 tggctggccg cccactcggt cacgacgctc ttctacctga tagccaggaa cacatcgccg
     1441 gccgcggcgc gcgttcacct aacgcgtctg gtacaattcc tgaaaattgc cgccgtggat
     1501 ggccgcgcaa tcgaacaggc gttggcgctg ccctacaggg actttgaaga cgcggtgcag
     1561 atgatggccg gagtccatgc cggcgtggag cacgttgtga cgcgcgatct atctggcttc
     1621 aaggccgggc cgttgcccac cctaacgccg ccggagttgt tagccttgtt gcggaggagt
     1681 taatggactg gaaactggaa gtcgtcatcg tgcccgtgtc ggacatcgac cgggcgcgcc
     1741 atttctacga gacgcaggtc ggcttcgtgg tcgatcacga cacggcgctg agcgaagacg
     1801 ttcgtatcgt ccagttgacg ccgcccggct c
//
LOCUS       DAOSMQ010000584         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_64103_length_1830_cov_3.656338, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000584 DAOSMQ010000000
VERSION     DAOSMQ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64103_length_1830_cov_3.656338"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1097)
                     /locus_tag="PK829_14430"
     CDS             complement(258..1097)
                     /locus_tag="PK829_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42461.1"
                     /translation="MRKKALIVAVAGLLFVAAALTLPARPNGGAARADVGPGAINLPI
                     ILKPGPTPSATLTPVPSPTPGFGENWLENGSFEDEHWTDLPPVEGYLINQEPRGWDLT
                     WLERGDIVWDLRTIHPNDPAVGRVSGIAEMLHKLARQLPPEEQAGGPRALILEGDQTY
                     KIFHHGGAFGSQLSQRVTLPAGRWRLTVPVQLHWHENLDPDDPTWDTYTAESGAWAIV
                     NGSWLGGWATARDMGDRRWFYHVVEFQLTEETEVEVLIRVKSIYLSAKDFFIDAVWLE
                     PIN"
     gene            1288..>1830
                     /locus_tag="PK829_14435"
     CDS             1288..>1830
                     /locus_tag="PK829_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOU42462.1"
                     /translation="MTLNLLHKHRDPAWLAEMTDWVRASLAARGLALLASPVAVRVLP
                     WSAVFRCATTGGDVFFKACAPSQAFEPALMRYLAERRPDDVLPVLAADLGRAWLLLPD
                     GGPVLRHTLTGTAADLVAHWSAILPRLAALQVALASDAAHLLSLGVMDRRPAVLPALY
                     DAVLADTGRWPLDHPDGLSRD"
BASE COUNT          277 a          645 c          590 g          318 t
ORIGIN      
        1 ggcggctaca cgccgtcgct cttctgggac ggctcggcca acggcttccc gacgagtatc
       61 gacgggttgg agatgattcc atagggaaag acgaataggg aaagaccaac cggcctgtgc
      121 ttcgccgccc agcggcccct acgccttgct ccacaggcca ggtgtagggg ccgcacgccg
      181 cagccacacc cgccccgcta cagagcaggc ttgtctcgcc aacgtccggc cattcctccc
      241 tcatccctca tccttcccta gttgatcggc tccagccaga ccgcgtcgat gaaaaagtct
      301 ttggcgctca gatagatact tttcacgcgg ataagcacct ccacctccgt ctcctcggtc
      361 aactggaact cgacgacgtg gtagaaccag cggcggtcgc ccatatcgcg ggccgtggcc
      421 cagccgccca gccaactgcc gttgacgatg gcccacgccc ccgactcggc cgtataggtg
      481 tcccaggtcg ggtcgtcggg atcgagattc tcgtgccaat ggagttgcac gggcacggtc
      541 agccgccagc ggccggccgg cagcgtcacc cgctgcgaca gctggctgcc gaacgcgccg
      601 ccgtggtgga agatcttgta ggtctgatcg ccttccagga tgagcgcccg cgggccgccg
      661 gcctgctcct ccggcggcag ctgccgcgcc agcttgtgga gcatctcagc gatgcccgac
      721 acgcggccga cggccgggtc gttggggtgg atggtgcgca gatcccagac aatatcgccc
      781 cgctccagcc aggtcaggtc ccagccgcgc ggctcctgat tgatgagata gccctcgacc
      841 ggcggcagat cggtccagtg ttcgtcctca aagctgccgt tctccagcca gttctcgccg
      901 aagccgggcg tgggcgaagg gacgggcgtc agggtggccg agggcgtcgg ccccggcttc
      961 aggatgatgg gtagattgat cgcgcccggc ccaacgtcgg ctcgggccgc gccgccgtta
     1021 ggccgcgccg gcaaggtgag ggccgcggca acgaacaaca ggccagctac cgctacgatt
     1081 agcgcctttt tgcgcatgag aatacctcct tccattgatg gcgtctgaat gcgcttgcct
     1141 tcgagattca ttatactcga tttatcccct ctcccgacgc gtcccccttc tgctggggcg
     1201 caacagagcg ccaggactaa gcggtgttcg ttgcgccctg gagccgagtc ttatagactg
     1261 gcctgaacaa atgctaagga cgaatccatg acgctcaacc tgctccacaa acaccgcgac
     1321 cccgcctggc tggcggagat gaccgattgg gtgcgggcga gcctggcagc gcgggggctg
     1381 gcgctgctgg cctcgccagt ggccgtgcgc gtgctgccgt ggtcggccgt ctttcgctgc
     1441 gccaccacgg gtggcgacgt cttcttcaag gcctgcgccc cgtcacaggc gttcgagccg
     1501 gcgctgatgc gctatctggc cgagcgccgc cccgacgacg tgttgcccgt gctggccgcc
     1561 gacctggggc gcgcctggct gctgctaccc gacggcggcc cggtgctgcg ccacacgttg
     1621 acgggcacgg ccgctgacct cgtcgcccac tggtcggcca tcctgccccg gctggcggcg
     1681 ttgcaagtcg ctctggccag cgacgcggcc catctgctct cgttgggggt gatggaccgg
     1741 cggccggcgg ttctgcccgc gctctacgat gccgtgctgg ccgacaccgg ccgctggccg
     1801 ctcgaccacc ccgacggcct atcgcgcgac
//
LOCUS       DAOSMQ010000585         1827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_64245_length_1827_cov_3.576749, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000585 DAOSMQ010000000
VERSION     DAOSMQ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64245_length_1827_cov_3.576749"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..686)
                     /locus_tag="PK829_14440"
     CDS             complement(258..686)
                     /locus_tag="PK829_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOU42463.1"
                     /translation="MTEKTLDRPFTVASFQTYMNEGRLMGSRNRATGEVFVPPRPIDP
                     ATYGEDMEWVELSGRGTLAAFTAVYIGTSAMIAAGYDRTNPYLAGVVALEEGPRVSAQ
                     IMGLDSKQPDIDWIGRPVRATFVERGEGEKRRAFLVFEPV"
     gene            complement(716..>1827)
                     /locus_tag="PK829_14445"
     CDS             complement(716..>1827)
                     /locus_tag="PK829_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOU42464.1"
                     /translation="EAIYIGNYSSDLFEGQGHTAPIMADWVGLAPRPATRVEDACASG
                     GVALRQGVLAIASGLYDVVLVGGIEKMSNLPIAGVTDALATAGDVSFEIPAGFTFPGF
                     YAAIATAYMHRYGASADALLRVGIKNHENGKLNPKAQFQARIADLMAGRIARAQQKGL
                     PVPSWQTEMDFLHDDGANPYIAWPLRLFDCSPVTDGAAAVLLVSEEIARRFSDDPLYI
                     IGTGQASDTSALHDRPELTALRAAQLAGQQAYAMAGVTPDDVKIAEVHDCFTIAEIVA
                     TEDLGFFAPGTGHEAAEEGLTSRQGPKPINTSGGLKSKGHPVGASGAAQVVEVWKQMH
                     GAAGERQVERDVDLAMTHNVGGTGQTCVVHIYERR"
BASE COUNT          275 a          630 c          577 g          345 t
ORIGIN      
        1 cgccatatta tacatcatac tcgttctttg tcgaatggtt agcgatcctt gcgggattgc
       61 ttcactcgcg tagccgaacg ctgcgctgac tgtctccggc ggcatcgcct cctggataac
      121 ccgctataac tcgaccagta gtgtgtacgg ggtcgtctgg tctcgtgccg tgccggcaag
      181 tgcgcatcgt cgaggaagtc agttgggccg ctgaaattgt ggctgcgccc tttgggtaac
      241 gcgcggcgat tgaggcgcta cactggctca aacaccagaa acgcccgccg cttctctcct
      301 tcgccccgct ccacgaacgt cgcccgcact ggccggccga tccagtcgat gtccggctgt
      361 ttgctgtcca gccccatgat ttgcgcgctg actcgcggcc cttcctccag cgccacaacg
      421 ccagccagat agggattcgt ccgatcatag ccggcggcga tcatcgccga cgtgccgatg
      481 tagaccgctg tgaacgccgc cagcgtgccc cgtcccgata gctcgaccca ctccatgtcc
      541 tcgccgtagg ttgccgggtc gatcggccgc ggcggcacaa agacctcgcc cgtcgcgcgg
      601 ttgcgcgacc ccatcagcct gccctcgttc atatacgtct ggaaagaggc cacagtgaag
      661 ggccggtcta gtgtcttttc ggtcatggct cacctcgtcc gtcgtctctc ctgcctcatc
      721 gccgctcgta aatatgcacc acgcacgtct gtcctgtgcc gccgacgttg tgggtcatgg
      781 ccagatcgac gtcgcgctcc acctgccgct cgccggccgc gccgtgcatt tgcttccaca
      841 cctcgaccac ctgggccgcg cccgacgcgc cgacggggtg gcccttgctc ttcagaccgc
      901 cggaggtgtt gatcggcttt ggtccctgcc ggctggtcag gccctcttcg gccgcctcgt
      961 gacccgtacc tggcgcgaag aagcccaggt cttccgtggc cacgatctcg gcgatggtga
     1021 agcagtcgtg gacttcggcg atcttgacgt cgtcgggcgt cacgccggcc atcgcatacg
     1081 cctgctgccc ggccagttgg gccgcgcgca gggcggtcag ttccggccgg tcgtggaggg
     1141 ctgaggtgtc gctggcctgc cccgtgccga tgatgtacag cggatcgtcg ctgaagcggc
     1201 gggcgatctc ctcgctgacc agcagcacgg ccgccgcgcc gtcggtgaca ggcgagcagt
     1261 cgaacagccg cagcggccag gcgatatagg gattggcccc gtcgtcgtgg aggaagtcca
     1321 tctcggtctg ccaggacggc acgggcagcc ccttctgctg cgccctggcg atgcgcccgg
     1381 ccatcaggtc ggcaatgcgc gcctggaact gggccttggg atttagtttg ccattctcgt
     1441 ggttcttgat gcccacgcgc aacagagcgt cggccgacgc gccatagcgg tgcatgtagg
     1501 ccgtggcaat ggcggcgtag aagccgggaa aggtgaagcc agccggaatc tcgaacgaca
     1561 cgtcgccggc cgtggccagc gcatcggtga cgccggcgat gggcaggttg ctcatcttct
     1621 cgatgccgcc gaccaacacc acgtcgtaga ggccggaagc gatggccaac acgccctgcc
     1681 gcaaggccac gccgccgctg gcacaggcat cctccacgcg cgtggccggc cgcggggcca
     1741 gccccaccca gtcggccatg atcggcgccg tgtggccctg cccctcgaag aggtcgctgc
     1801 tgtagttgcc gatgtagatc gcttcga
//
LOCUS       DAOSMQ010000586         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_64838_length_1815_cov_3.940909, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000586 DAOSMQ010000000
VERSION     DAOSMQ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64838_length_1815_cov_3.940909"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PK829_14450"
     CDS             <1..1438
                     /locus_tag="PK829_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU42465.1"
                     /translation="LTMAYGRDESRMVFTEKLKLAKHVPLGELIYLDRERCIQCARCT
                     RFQAEVVGDPVIGFHNRGRRLEIVTFSDPGFDSIWSGNTTDICPVGALTTADFRFGAR
                     PWELNPVASICTHCPVGCNLTMSTRREAASGGRNVIKRIMPRQNETVNEIWICDKGRF
                     VHHFADHPDRLTQPLVRRDGRLEPVSWDDALDFVAGRLQQHKDAVAGVAGDRLSNEDY
                     FQFQRLFRQGLGSGNIDLAERRLAGADVVARVGLARGSNLKDLGRGDAILVVAADLHE
                     EAPVWWLRVKQAAERGATLMVLNARPTRLDAYARFVRTYQNGAALTAVRELLALAHVD
                     TGDAPADESGAMANALMEANNLVVFYGAEGLSYDQTDALARMLANLLLLKRSEEAQPF
                     AGRTNNGLVAVGRHANGQGARDMGVHPVYAPGYRAAPSAGLNADEIYAAAADGGLRAL
                     FVAGADPVGDGLMEAAADWPSSSSRSYS"
     gene            1462..>1815
                     /locus_tag="PK829_14455"
     CDS             1462..>1815
                     /locus_tag="PK829_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU42466.1"
                     /translation="MVLPAQSWAEREGTFTNGERRVQRYYPGIPAMGEARPDWQILAQ
                     LGERVGLGKPPFAASLVFKEIAAAVPQYAGMDYRSLANVEPQWPIIGRSDMYYGGTAY
                     DNRWGLGQQWPAAAES"
BASE COUNT          277 a          658 c          619 g          261 t
ORIGIN      
        1 cctgaccatg gcctatggcc gcgacgagag ccgcatggtc ttcaccgaga agctcaagct
       61 ggccaagcac gtgccgctgg gcgaactcat ctacctcgac cgcgagcgct gcatccaatg
      121 cgcccgctgc actcgcttcc aggctgaagt ggtcggcgac ccggtcatcg gcttccacaa
      181 ccgcggccgg cggctggaga tcgtcacctt ctccgacccc ggcttcgata gcatctggag
      241 cggcaacacg accgacatct gccccgtggg cgcgctgacg acggccgact tccgctttgg
      301 cgcgcggccg tgggagttga accccgtggc cagcatctgc acccactgcc ccgtcggctg
      361 caacctgacc atgagcaccc gtcgcgaggc ggccagcggc ggccggaacg tcatcaagcg
      421 catcatgccg cgccagaacg agaccgtcaa cgagatctgg atctgcgaca agggccgctt
      481 tgtccaccac ttcgccgacc accccgaccg gctgacccag ccgctggtgc gccgcgacgg
      541 ccggctggaa ccggtgtcgt gggatgacgc cctcgacttc gtcgccgggc ggctgcaaca
      601 acacaaggac gctgtcgccg gtgtggctgg cgaccggctg agcaatgagg actacttcca
      661 gttccagcgc cttttccgcc aggggctggg cagcggcaac atcgacctgg ccgagcggcg
      721 gctggccggc gcggacgtgg tcgcccgcgt gggcctggcc cgtggcagca acttgaaaga
      781 cctggggcgc ggcgacgcca tcctcgtcgt cgccgccgac ctgcacgagg aagcgccggt
      841 gtggtggctg cgcgtgaagc aggccgccga gcgcggcgcg acgctgatgg tgcttaacgc
      901 ccggccgacg cgccttgacg cctacgcccg cttcgtccgc acttaccaga atggcgccgc
      961 gctaaccgcc gtgcgcgagt tgctggccct ggcccacgtg gacaccggcg acgcgccggc
     1021 cgacgaatcc ggcgcgatgg ccaatgccct catggaggcc aataacctgg tcgtcttcta
     1081 cggggccgag gggctgagct acgatcagac cgacgccctg gcgcggatgc tggccaatct
     1141 gctgctgctg aagcgcagcg aggaggcgca gcccttcgct gggcgcacca acaacggtct
     1201 cgtggccgtc ggccggcacg ccaacggcca gggcgcgcgc gacatgggcg tccaccccgt
     1261 ctacgcgccc ggctaccgcg ccgcgcccag cgccggcctg aacgccgatg agatctacgc
     1321 cgcggcggcc gacggcggcc tgcgcgccct cttcgtcgcc ggggccgatc ccgtgggcga
     1381 tgggctgatg gaggccgcgg cggactggcc ttcctcgtcg tccaggagct attcctgacg
     1441 ccgacggccg agctggccga cgtggtcttg cccgcccaga gctgggccga gcgcgagggc
     1501 acgttcacca acggcgagcg gcgcgtccag cgctactacc ccggcatccc ggccatgggc
     1561 gaggcgcggc ccgactggca gattctggcc cagttaggcg agcgcgttgg cctgggcaag
     1621 ccgcccttcg ccgccagcct cgtcttcaag gagatcgccg ccgccgtgcc acagtacgcg
     1681 ggcatggact accgctcgct ggccaacgtg gagccgcagt ggcccatcat tggccgcagc
     1741 gatatgtact atggcggcac ggcctatgac aaccgctggg gtctggggca gcagtggccg
     1801 gccgcggccg agtct
//
LOCUS       DAOSMQ010000587         1813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_64941_length_1813_cov_2.990899, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000587 DAOSMQ010000000
VERSION     DAOSMQ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64941_length_1813_cov_2.990899"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PK829_14460"
     CDS             complement(<1..832)
                     /locus_tag="PK829_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU42467.1"
                     /translation="MTTLPLIPRRVFFGNPDRAQVTISPDGTHLAWLAPRDGVLNVWV
                     APRQNPADARPVTGDTGRGIRFYAWAYSGDILYIQDKGGDENWRVYSADIDTGEVGDL
                     TPFEGVAAQILALSHRRPDEIVVAMNDRNPQYHDIYRVNLTNGERTLLLEHERFIGVD
                     VDDDLAVRLGEQMTPDGGLAVYRPEGGDWAEWQTFPPADMLTASVVGFSQDGGTLYAR
                     DSRGRDTAALVAIDMATGAKTVLAEDARADADQVLRHPTEHHVQAVAFNYERKHWQVL
                     D"
     gene            complement(945..1217)
                     /locus_tag="PK829_14465"
     CDS             complement(945..1217)
                     /locus_tag="PK829_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42468.1"
                     /translation="MEPELILRLFLIVLGVVFLVLSFAGAILELARHAGRTEAAGAPN
                     PLDSVTKLAEALGALFAALAGAPLWLGLGGFGIILILVGALVPFNF"
     gene            complement(1245..>1813)
                     /locus_tag="PK829_14470"
     CDS             complement(1245..>1813)
                     /locus_tag="PK829_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOU42469.1"
                     /translation="EAWPAAFEAEIAGLLGGVDEPLLARYQALTRRIAAMPRGRDDYG
                     LIHFDAHEANLHIDGDRVVLFDFDDCVYNWFIADIAIVLFYKVANHDAPERVAAGFLP
                     GFLRGYAAENRLDPRWLAAIPDFLTTREIDLYAIIRRSYDLPEVTPAAVEAIPHDWSR
                     RFMRGRRERLLAEQPYLDFDFSALADYL"
BASE COUNT          298 a          571 c          656 g          288 t
ORIGIN      
        1 ggtcgagcac ctgccagtgc ttgcgctcat agttgaaggc caccgcctgc acgtgatgct
       61 ctgtcgggtg gcgcagtacc tggtcggcgt cagcgcgggc gtcttcagcc agcaccgtct
      121 tcgcgcccgt ggccatgtcg atggccacca gcgcggcggt gtcgcggccg cggctgtcgc
      181 gggcatagag cgtgccgccg tcctggctga aaccaacgac cgacgccgtc agcatgtctg
      241 ccggcggaaa agtctgccac tcggcccagt cgccgccctc tggccggtag actgccagcc
      301 cgccgtcggg cgtcatctgt tcgcccagac ggacagccag atcgtcgtcc acgtccacgc
      361 cgataaagcg ttcgtgctcc agcagcagcg tccgctcgcc attggtcagg ttgacgcggt
      421 agatgtcgtg gtactgtggg ttgcggtcgt tcatggcgac gacgatctca tccggccgcc
      481 ggtggctgag ggcgagaatc tgcgcggcca cgccctcgaa cggcgtcagg tcgcccactt
      541 cgccggtgtc gatgtcggcc gaatagacgc gccagttctc gtcgccgccc ttgtcctgaa
      601 tgtagaggat gtcgccactg taggcccagg cgtagaagcg gatgccgcgg ccggtgtcgc
      661 cggtgacggg gcgggcgtcg gccggatttt ggcgcggggc gacccagacg ttgagcacac
      721 cgtcgcgcgg ggccagccag gccagatgcg tcccgtccgg gctgatggtc acctgcgccc
      781 ggtcggggtt gccgaagaag acgcggcggg gaatgagagg gagagtcgtc attggggttc
      841 ctttcggtga taggggacag tgggtcagtg ggtcagtggt cagtaggtca gtgggtcagt
      901 aggccagtaa ggcggtcggc ggtcggccgt ccgcggtcgg cgatctagaa gttaaacggc
      961 accagcgcgc ccacgagaat gagaatgatg ccgaagccgc ccaggcccag ccacagcggt
     1021 gcgccggcca gcgcggcgaa gagcgccccc agggcctcgg ccagcttggt gacactgtcc
     1081 agcggattgg gcgcgccggc tgcttctgtg cgtccggcgt ggcgtgccag ttccaggatg
     1141 gccccggcga acgacaggac aagaaagacg acgcccagga cgatgagaaa gagccgcaag
     1201 atgagttccg gttccacgtg gcctcctttc gcgttgcgtt gcgcctaaag ataatccgcc
     1261 agcgccgaaa agtcgaaatc gagatagggc tgctccgcca gcagccgctc gcgccggccg
     1321 cgcatgaacc ggcgcgacca gtcgtggggg atggcttcga cagccgcggg cgtcacctcc
     1381 ggcaggtcgt agctgcggcg aatgatggcg tagaggtcga tctcccgcgt cgtcagaaag
     1441 tcggggatgg ccgccagcca acgcggatca agccggttct cggcggcgta gccgcgcagg
     1501 aagccgggca ggaagccggc ggcgacgcgc tccggcgcgt cgtggttggc caccttgtag
     1561 aagaggacga tggcgatgtc ggcgatgaac cagttgtaga cgcagtcgtc gaagtcgaac
     1621 aacaccaccc ggtcgccgtc gatgtggagg ttggcctcgt gggcgtcgaa gtggatcagg
     1681 ccgtagtcgt cccggccgcg gggcatggcc gcgatgcgcc gcgtcagggc ctggtagcga
     1741 gccagcagcg gctcatcgac gccgcccagc aagccggcga tctcggcctc aaaggcggcc
     1801 ggccacgcct cgc
//
LOCUS       DAOSMQ010000588         1810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_65057_length_1810_cov_5.318519, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000588 DAOSMQ010000000
VERSION     DAOSMQ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65057_length_1810_cov_5.318519"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PK829_14475"
     CDS             <1..727
                     /locus_tag="PK829_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU42470.1"
                     /translation="SPIAGTTRDAIDERLRWHGQEFTLIDTAGIRRRGKIDQGVEKYS
                     VLRALKTLRRADVALLLIDGEEGVTAQDAHIGGMLTEENVGVIILVNKWDIVEKDAYT
                     MPAYEKTARNELAFLSYAPLLFISAETGQRLNKIFPTVLEVQAARHQRVGTGELNDLL
                     RDLIANHPPPTKAGTQLKFYYATQVAVAPPTFVFFVNRPEMIHFGYQRYIENRIREQF
                     TFSGTPIRLLFRGRKDERPGRDD"
     gene            complement(724..>1810)
                     /locus_tag="PK829_14480"
     CDS             complement(724..>1810)
                     /locus_tag="PK829_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU42471.1"
                     /translation="KGLQAQPDVAVHVCHATLWQGIEDRVQQASGGWRNPRFWGRVVS
                     AYRDLVRAHAATPPYDVMLIGYPGQFDAYLGRRLAHGRGAPVALDILMSLHLVAEERG
                     LVAAHPTTGALLFRLERGGLRRPDLLIAENAEYGGYIAGKYHLPPERFRYVPHGADET
                     IFHPRPVRPPDDRFRVTYHGGYLPSHGLDAILGAAARLRDQADVGFEFYGSGPEKERI
                     VQQAAGLGLRNVTFHGFVDQDELLDGLAAAHVCLGVFGVTRQANFTVQNKVWEGLAMG
                     RPVISGDSPTIRAALADRREVYLVARNSPEALAAGLTELRADPALRENIAAAGHARYL
                     AGNSTTAIGRQMADALRGLVAAWAGER"
BASE COUNT          308 a          626 c          623 g          253 t
ORIGIN      
        1 cagccccatc gccgggacga cgcgcgacgc catcgacgag cggctgcgct ggcatgggca
       61 ggaattcacg ctgatcgaca cggccggcat ccgccgccgc ggcaagatcg accagggcgt
      121 ggagaagtac agcgtcctgc gcgccctgaa gacgctgcgc cgggccgacg tggccctgtt
      181 gctcatcgat ggcgaggaag gcgtcaccgc gcaggacgcc cacatcggtg gcatgctaac
      241 cgaggagaac gtcggcgtca tcatcctggt caataagtgg gacattgttg aaaaggacgc
      301 ctacaccatg cccgcctacg agaagacggc acgcaacgaa ctggccttcc tgtcctatgc
      361 gcccctgcta ttcatctccg ccgagacggg ccagcggctg aacaagatct ttccgacggt
      421 gctggaggtg caggcggcgc gtcaccagcg cgtgggcacg ggcgagctaa acgacctctt
      481 acgcgacctg atcgccaacc acccgccgcc gaccaaggcg ggcacgcagt taaagttcta
      541 ctacgccacg caagtggccg tggccccgcc aacgttcgtc ttcttcgtca accggccgga
      601 gatgatccac ttcggctacc agcgctacat cgaaaaccgc atccgggagc agttcacttt
      661 cagcggcacg cccatccggc tgctgttccg cggccgcaag gacgagcgcc ccggccgcga
      721 cgactagcgc tcgcccgccc acgcggcgac gaggccccgc agcgcgtcgg ccatctgccg
      781 gccaatggcg gtggtgctgt tgccggccag gtagcgggcg tggccggcgg cggcgatgtt
      841 ctcgcgcagc gccgggtcgg cccggagttc agttagtccg gccgccaggg cctccgggct
      901 attgcgagcc accaggtaga cttcccgccg gtcggccagc gcggcgcgga tggttggcga
      961 atcgccgctg atgaccggcc ggcccatggc cagcccctcc caaactttgt tctgcacggt
     1021 gaagttggcc tgccgtgtca cgccgaagac acccaggcag acgtgcgccg ccgccagccc
     1081 gtcgagcaac tcgtcctgat cgacaaagcc gtggaaggtg acgttgcgca gccccagccc
     1141 ggccgcctgc tggacaatgc gctccttctc cggcccgctg ccgtagaact cgaagccgac
     1201 atcggcctga tcacgtagcc gtgccgccgc gccgagaatg gcgtccaggc cgtgcgacgg
     1261 cagatagccg ccgtggtaag tgacgcggaa gcggtcgtcg ggcgggcgca ccggccgggg
     1321 atggaagatg gtctcatccg cgccgtgggg cacgtagcgg aagcgctcag gcggcaggtg
     1381 gtacttgccg gcgatatagc cgccgtactc cgcattctcg gcgatgagca ggtcgggccg
     1441 gcgcaggccg ccacgctcca gccggaagag cagcgccccc gtggtcgggt gggcggcgac
     1501 aaggccgcgc tcctcggcca ccagatgcag cgacatgagg atgtcgagag ccaccggcgc
     1561 gccgcggccg tgggccaggc ggcggccgag gtaggcgtcg aactggccgg ggtagccgat
     1621 gagcatcacg tcgtaaggcg gcgtggcggc gtgggcgcgg acgaggtcac gataggccga
     1681 gacgacccgg ccccagaagc gcgggttgcg ccagccaccg ctggcctgct ggacgcgatc
     1741 ctcgatgccc tgccacagcg tcgcatggca gacatgcacg gccacgtccg gctgggcctg
     1801 caagcccttg
//
LOCUS       DAOSMQ010000589         1809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_65117_length_1809_cov_2.920182, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000589 DAOSMQ010000000
VERSION     DAOSMQ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65117_length_1809_cov_2.920182"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PK829_14485"
     CDS             complement(<1..1197)
                     /locus_tag="PK829_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOU42472.1"
                     /translation="MTDLGDNIILTGFMGTGKTTVGRLLAARLGRDFVDSDDLIVARA
                     GKPVADIFRDEGEERFRAWEADTAAELAGRRGLVIATGGRLMLDAANAATLGVTGPVF
                     CLSASADEILTRLAADGVDRPLLAGDAAGARVAALLRQRAVGYARFRPVATDGRSPAA
                     VAGDIAAALAAGLRDTLTVRHPGGSYKVVVGEGLLAEAAGLARGPAVVITDSEVGPRH
                     AASLGPLPGLTMPAGERHKTLDTVRALYDGLLAAGLGRDGTVIGLGGGVVGDVAGFVA
                     ATYLRGIDFALCPTSLLAMVDASIGGKTGVDLPQGKNLVGAFAQPRGVLADVGTLATL
                     PAAEFTAGLAEVAKHGLIADPLLWQRLMMEEWHIDPALLAADRLRRADLQSLLVRAIG
                     VKRDVVE"
     gene            1307..>1809
                     /locus_tag="PK829_14490"
     CDS             1307..>1809
                     /locus_tag="PK829_14490"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU42473.1"
                     /translation="MSKHKNLIVVAIIVVLSLALAACNAAPGANSAAIAPAAQTGSTV
                     SAGGITVVGQGTAYGQPDQATVVVGVETFAATVSEASAQNQTTLDNVMAALTAAGIAN
                     EDIQTTNYSLYAEQIYGDKGPEGIAGYHVSNQVNVKIRDISLVGDVLSAVTEAGANAI
                     YGVNFSVA"
BASE COUNT          309 a          654 c          595 g          251 t
ORIGIN      
        1 ttcgaccacg tcgcgcttga cgccgatggc ccgcaccagc agcgattgca gatcggcccg
       61 ccgcagccgg tcggcggcta gcagcgccgg gtcgatatgc cactcctcca tcatcagccg
      121 ttgccacagc agcgggtcgg cgatcaggcc gtgcttggcc acctcggcca ggccggcggt
      181 gaactcggcc gcgggcagcg ttgccagcgt gcccacgtcg gccaacacgc cgcgcggttg
      241 ggcaaacgcg ccgacgaggt tcttaccctg gggcaaatcc acgcccgtct tgccgccgat
      301 gctggcatcg accatggcca gcaggcttgt tggacagagg gcgaagtcga tgccgcgcag
      361 gtaggtggcg gccacgaaac cggccacgtc gccgacgacg ccaccgccca ggccgatgac
      421 cgtgccatcg cgccccagcc cggcggccag caggccgtcg taaagagcgc gcaccgtgtc
      481 cagcgtcttg tgccgttcgc ccgcaggcat ggtcagcccg ggcagcgggc cgagacttgc
      541 ggcatggcgc gggccaacct cgctatcggt gatgaccacg gccgggccgc gggccagccc
      601 ggccgcctcc gccagcagcc cctcgccgac gacgaccttg tagctgccgc cggggtggcg
      661 cacggtcagc gtgtcgcgca gcccggcggc caacgccgcg gcgatgtcgc cggcgacagc
      721 cgcgggcgaa cggccgtcgg tggccaccgg ccggaagcgg gcatagccga cggcccgctg
      781 ccgcagcagc gcggccaccc gcgccccggc ggcgtcgccc gccagcagcg gtcgatccac
      841 gccgtccgcg gccaggcggg tcaaaatttc gtcggccgag gcgctgaggc agaagaccgg
      901 ccctgtgacc cccaacgtgg cggcattggc cgcgtcgagc atcagccgcc cgcctgtggc
      961 gatgaccagc ccgcgccgcc cggccagttc ggcggcggtg tcggcctccc aggcgcgaaa
     1021 gcgctcttcg ccctcgtcgc ggaaaatatc ggccacgggc ttgccggcgc gagcgacaat
     1081 gagatcgtcg ctatcaacga agtcgcgccc cagccgcgcg gccagcaggc ggccgacggt
     1141 cgtcttgccc gtgcccatga agcctgtcag gatgatgttg tcgcccaggt cagtcattgg
     1201 ccctaattat ccaccaaatg gccacttttg cggaaccatt ggcagcatcc cggcgtccta
     1261 tcagcagacc ggtaacggtc aaaaacgctt gtaaggagag taacagatgt ctaagcacaa
     1321 gaatctgatc gtcgtcgcta tcatcgtcgt cctgagtctg gctctggcgg cctgcaacgc
     1381 cgcgcccggc gccaatagcg ccgccattgc gcccgccgcg cagacgggtt cgaccgtcag
     1441 cgccgggggc atcactgtcg tcggtcaggg cacggcctat ggccagcccg accaggccac
     1501 cgtcgtcgtt ggcgtggaga ctttcgccgc gaccgtgagc gaggccagcg cgcagaatca
     1561 gacgaccctc gacaacgtca tggccgcgct gacagccgcc ggcatcgcca acgaggacat
     1621 ccagacgacc aactacagcc tctatgccga acaaatctac ggcgacaaag ggccggaggg
     1681 aattgccggc taccacgtca gcaaccaggt caacgtcaag attcgcgaca tcagccttgt
     1741 tggcgatgtc ctcagcgcgg tgaccgaggc gggggccaac gccatctacg gcgtcaactt
     1801 ctccgtggc
//
LOCUS       DAOSMQ010000590         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_65711_length_1797_cov_4.502870, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000590 DAOSMQ010000000
VERSION     DAOSMQ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65711_length_1797_cov_4.502870"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PK829_14495"
     CDS             <1..704
                     /locus_tag="PK829_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42474.1"
                     /translation="AGEHSDRLKKRALFILVPLWVGSRHGGRRGLARPAPATLAYFGG
                     LGLAFMLVEIPTMQKLTVYLGQPVYSLAVVLFALLLFSGLGSWWSGRWPAESIATRLR
                     RVFPLLTVFLLLHALLSSAVLARTLWFSLPARLLVAVLLLSALGFLMGMPFPTGIRWA
                     GQRQPTVVPWLWGINGVTSVLGSALATALAIHVGFRLTLLTAALVYAAVGALFVLATR
                     RAATSVPDLELGTTD"
     gene            747..>1797
                     /locus_tag="PK829_14500"
     CDS             747..>1797
                     /locus_tag="PK829_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOU42475.1"
                     /translation="MTSLTQSHLYREIHEQPDVLRRLLDEEEATAQALADLVRGRDIT
                     HVVIAARGTSDNAGRYAQYVLGSMNGLPVGLAAPSLFTIYQRPPRFGNALVLGISQSG
                     QSPDIVAVLAEARRQGATTAVLTNRVDSPLAAQGDVVINLRAGEERAVAATKTYTAEL
                     AAVALLSVALSGDAEARAALWATPDAVAATLAMNATIAAVAPRYRYMQRCVVIGRGYN
                     YATAFETALKLKEMTYTIVEPYSSADFLHGPLAMLEPGFPVIVIAPSGRLAAEAESFI
                     GTLRERQAEVIAVSDDAAVLAAARVPLALPARVPEWLSPLTAIIPGQLLALQLAHARD
                     FDVDAPRAIRKVTETV"
BASE COUNT          232 a          665 c          620 g          280 t
ORIGIN      
        1 tcgccggcga gcacagcgat cgcctgaaaa agcgcgccct gttcatcctg gtgcccctct
       61 gggtgggcag ccggcatggc ggccggcgcg gcctggcccg ccccgccccg gccacgctgg
      121 cctatttcgg cggcctgggc ctggccttca tgctggtcga gattccgacg atgcagaagt
      181 tgaccgtcta cctgggccag ccggtctact cgctggcggt ggtgctgttc gcgctgctgc
      241 tgttcagcgg cctgggcagc tggtggagcg gccgctggcc ggccgagagc atcgccactc
      301 gcctgcggcg cgtcttcccg ctgctgaccg tctttctgct gctccatgcc ctgctcagca
      361 gcgccgtgct ggcgcggacg ctgtggttta gcctgccggc gcggctgctg gtggccgtgc
      421 tgctgttgtc ggccctgggc ttcctgatgg gcatgccctt ccccaccggc atccgctggg
      481 ccgggcagcg ccagccgacg gtcgtgccct ggctgtgggg catcaacggc gtcacgtcgg
      541 tgctcggctc ggccctggcc acggcgttgg ccattcatgt cggcttccgg ctgacgctgc
      601 tgaccgccgc gctggtctat gccgccgtcg gcgcgctgtt cgtgctggcc acgcgccgcg
      661 cggcgacgag cgtgcccgac ctcgaactgg gcacgaccga ctaagcccgg ccgggcgacc
      721 ggccgccaag cgaacgagag acccccatga catccctgac ccaaagccac ctctaccgcg
      781 aaatccacga gcagcccgac gtgctgcgcc gcctgctgga cgaggaggaa gccactgccc
      841 aggctctggc cgacctcgtc cggggccggg acatcaccca tgtcgtcatc gccgcccgcg
      901 gcaccagcga caacgccggc cgctacgccc agtacgtcct cggctccatg aacggcctgc
      961 cggttgggct ggccgcgccg agcctgttca ccatctacca gcgcccgccg cgcttcggca
     1021 atgccctggt gctgggcatc agccagagcg gccaaagccc cgacatcgtt gctgtgctgg
     1081 ccgaggcgcg ccggcaaggg gccaccaccg ccgtgctgac caaccgcgtc gattcgccgc
     1141 tggccgcgca gggggacgtg gtcatcaatc tgcgcgccgg cgaggagcgc gccgtggcgg
     1201 ccacgaagac ctacaccgcc gaactggccg cggtggcgct actcagcgtg gccctgagcg
     1261 gcgatgccga ggcgcgggcg gcgttgtggg ccacacccga cgccgtggcg gcgacgctgg
     1321 ccatgaacgc gaccatcgcc gccgtggccc cgcgctatcg ctacatgcag cgctgcgtgg
     1381 tcatcggccg cggctacaac tacgccaccg ccttcgagac ggcgctgaag ctgaaggaga
     1441 tgacctacac catcgtcgag ccgtacagta gcgccgactt tctccacggg ccgctggcca
     1501 tgctggagcc gggcttcccg gtcatcgtca tcgccccgtc ggggcggctg gcggccgagg
     1561 cggagagctt catcggcacg ctgcgcgagc gacaggcgga ggtcatcgcc gtcagcgacg
     1621 acgcggccgt gctggcggcg gcgcgcgtgc cgctggcgtt gccggcccgt gtgccggagt
     1681 ggttgtcgcc gctgacggcc atcatccccg gccaactcct ggccctgcaa ctggcccacg
     1741 cgcgcgactt cgacgtcgac gcgccgcggg ccattcgtaa ggtgacggag acggtct
//
LOCUS       DAOSMQ010000591         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_66454_length_1783_cov_5.190394, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000591 DAOSMQ010000000
VERSION     DAOSMQ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66454_length_1783_cov_5.190394"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PK829_14505"
     CDS             <1..261
                     /locus_tag="PK829_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOU42476.1"
                     /translation="VVGATYPAELAAARALAPAANFLIPGIGAQGGDLAAAVAHGAGA
                     AAGPVINAGRSVLYASAGLDFAAAARAQAARLREAINALREG"
     gene            435..713
                     /locus_tag="PK829_14510"
     CDS             435..713
                     /locus_tag="PK829_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HOU42477.1"
                     /translation="MKRPTNCVHVDEIQEVTPNTFGCEDCLKTGSTWVHLRVCLTCGY
                     VGCCNSSPNKHSSRHAKETGHHVVMSMEGGEEWMWCYEDEDFIIPRRR"
     gene            710..>1783
                     /locus_tag="PK829_14515"
     CDS             710..>1783
                     /locus_tag="PK829_14515"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU42478.1"
                     /translation="MTDLFGRPPSPALSNTRATPTARDTGSIPAGERRVITVLFCDVV
                     GSTSLASRLDPEEWAEVMNDAFRYMITPVEEYGGMVARLMGDAVLAFFGAPQAHEDDP
                     QRAILAGLGIVNGMRPFIRQFEQDYGLAFNVRVGINTGPVVVGEMGTGQLGEYTAMGD
                     AINLAARMEQTAAPGTVQISDDTYRHVAPLFDVWDLGGIEVKGKDEPVHAYRVLGLEA
                     QPGRLRGLEGIHAPLVGRDRELGRLRDVLTALRDGQSAIVYLVGEAGLGKSRLIAEAR
                     QMWREQLAAASRDVDPQRVGPQSVGPQNVGPQSVDPQIADPQRLDPLWRGWTEFVAVS
                     FGASRPYDLLKRQVRHFCNIRETD"
BASE COUNT          288 a          616 c          609 g          270 t
ORIGIN      
        1 gtcgtcggcg cgacctaccc ggccgaactg gccgcggcgc gggcgctggc cccggcggcc
       61 aacttcctca tccccggcat cggcgcgcag gggggcgacc tggccgcggc cgtggcccac
      121 ggggccggcg cggcggccgg gccggtcatc aatgccgggc gcagcgtgct ctatgcctcg
      181 gccggcctcg acttcgccgc cgccgcccgc gcccaggcgg cgcggttgcg ggaggcgatc
      241 aacgcgctaa gggaaggcta ggccgcgtct ccccgcgttt gacaccattc cccgttgcgc
      301 gtacactcaa attgcgcccg tgccggcccc ttcatggcct ggcgacgccg caaccgacag
      361 ccttaaacag gagaaaggca atgaacgatg ccgcgattgt cctggatgcc atccgccgcg
      421 tgaaggcggg tgaactgaag cgtccgacca actgtgtgca tgttgatgag attcaggaag
      481 tgacgccgaa cactttcggc tgtgaggact gcctgaagac tggcagtacg tgggtacatt
      541 tgcgcgtctg cctgacctgt ggctatgtcg gttgctgcaa cagttccccc aacaagcact
      601 cctcgcgcca cgctaaagag acgggtcacc atgtcgtcat gtcgatggag ggcggcgaag
      661 agtggatgtg gtgctacgag gatgaggact ttatcattcc gcgacgccga tgaccgatct
      721 gttcggccgc ccgccctcgc cggcgcttag caatacgcgc gccacgccaa cggcccgcga
      781 cacgggcagc atccccgccg gcgaacgccg ggtcatcacc gtcctcttct gcgacgtcgt
      841 cggctcgacc tcgctggcca gccgcctcga cccggaagag tgggctgagg tgatgaacga
      901 tgccttccgc tacatgatca ccccggtaga ggagtatggc ggcatggtcg cccggctcat
      961 gggcgacgcc gtgctggcct tcttcggcgc gccccaggcc cacgaggacg acccccaacg
     1021 cgccatcctg gccgggctgg gcatcgtcaa cggcatgcgc ccgttcattc gccagttcga
     1081 gcaggactac ggcctggcct tcaacgttcg cgtgggcatc aacaccggcc ctgtcgtcgt
     1141 tggcgagatg ggcacggggc agctgggcga gtacacggcc atgggcgacg ccatcaacct
     1201 ggccgcgcgc atggagcaga cggccgcgcc cggcacggtg cagatcagcg acgacaccta
     1261 ccgccacgtc gccccgctgt tcgatgtctg ggacctgggc ggcatcgagg tcaagggcaa
     1321 ggacgagccg gtgcacgcct accgcgtgct ggggctagag gcgcagcccg gccgcctgcg
     1381 cgggctggag ggcatccacg ctccgctggt cggccgcgac cgtgaactgg gccgcctgcg
     1441 cgacgtgctg accgccctgc gcgacggcca gagcgccatc gtctacctcg tcggcgaggc
     1501 gggattgggc aagagccggc tcattgccga agcgcgacag atgtggcgcg agcagttggc
     1561 cgccgccagc agggacgtgg acccgcagag ggtgggcccg cagagcgttg gcccgcagaa
     1621 cgtgggcccg cagagcgttg acccgcagat agctgacccg cagcggctag accccctctg
     1681 gcgcggctgg acggaattcg tagccgtctc cttcggggcc agccggccct atgacctgct
     1741 gaagcgccag gtgcgccact tctgcaacat ccgcgagacc gac
//
LOCUS       DAOSMQ010000592         1774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_66933_length_1774_cov_3.630599, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000592 DAOSMQ010000000
VERSION     DAOSMQ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66933_length_1774_cov_3.630599"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1021
                     /locus_tag="PK829_14520"
     CDS             167..1021
                     /locus_tag="PK829_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU42479.1"
                     /translation="MIEVRHLTKRFGRFMAVQDLSFTVAQGEAVALWGANGAGKTTAV
                     RCLLNLFPYEGEITVNGLDARRQSKAVRRLIGFVPQELSFHDDMTVAETLVFYARLKK
                     VPDGYDFEPLLERLRLTTHVGHRVGELSGGLKQRLALAVALLSDPPILILDEPTASLD
                     IRSREEFLVLLRGLKRAGKTMVFSSHHLEEITALADRVLLLEDGRLVVDAPPDQLERQ
                     LNWETTLHLYMADTAIDPALQTLAELGMPVSRNGRGLRVQVAPGSKGKVLQALHDVGV
                     RVDDFTVE"
     gene            1022..>1774
                     /locus_tag="PK829_14525"
     CDS             1022..>1774
                     /locus_tag="PK829_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU42480.1"
                     /translation="MDFENVLILIQKELRDALRNRWFLLYAVAFAALSLALAWLSVSG
                     AGSYGVAGFGRTTAGLINLVLLIVPLMGLTLGAMSLAGEREKGTLVYLLAQPISAAEL
                     LLGKFIGLALALTAALVIGFGLTGLMMVVSGSGGDFIIFATLMALAILLAVASLSVGF
                     LISAAVRRAATAVGLALFLWLVLVYFGDLGLMGTAMVVQMDVGQLLTLALVNPLQVFK
                     IAAVLNLRDNLEVLGPAGIYAFRTYGGALWPML"
BASE COUNT          255 a          568 c          623 g          328 t
ORIGIN      
        1 cagcaacgcc ccgctgccca cgggcggcga agcgggcgcg ctgtggccgg tgctgctgtt
       61 gccattggcg ctgctgctgc tggccggcct gggccgactc ggccgccgcc actatcattt
      121 gcctgaggcc ggccgcgccg agcgcccggt cgtggttgaa taagccatga ttgaagttcg
      181 ccatctgacc aaacgctttg gccgcttcat ggccgtgcaa gacctgtcgt tcaccgtcgc
      241 ccagggcgaa gcggtggccc tctggggggc caacggcgcg ggcaaaacga cggccgtccg
      301 ttgcctgctc aacctcttcc cctatgaggg ggagatcact gtcaacgggc tggacgcgcg
      361 ccggcagagc aaggcggtgc gccgtctcat cggcttcgtg ccccaggaac tgagtttcca
      421 cgacgatatg accgtggccg agacgcttgt cttctacgcc cggctgaaga aagtgcccga
      481 cggctatgac ttcgagccac tgctggagcg gctgcgcctg accacccacg tcggccaccg
      541 cgtcggcgag ctgtcgggcg ggctgaagca acggctggcg ctggccgtgg ccttgctgtc
      601 tgacccgccc atcctcatcc tcgacgagcc gacggccagc ctggacattc gtagccgcga
      661 ggagttcctg gtgctgctgc gcgggctgaa gcgggccggc aaaacgatgg tcttttcctc
      721 tcatcatctg gaggagatca ccgccctggc cgaccgggtg ctgctgctgg aagacggccg
      781 gctggtggtc gatgccccgc ccgaccagct ggagcggcaa ctgaactggg agacgacgct
      841 acacctttac atggccgaca cggccatcga cccggcgttg cagacgctgg cagaactggg
      901 catgcccgtc agccgcaacg gccgcgggct gcgcgtgcag gttgcgccgg gcagtaaagg
      961 caaggtgctg caagcgctgc acgatgtcgg cgtgcgggtc gatgacttca cggtggagta
     1021 aatggacttc gagaacgtac tgatcctcat ccagaaagaa ctgcgcgacg cgctgcgcaa
     1081 ccgctggttc ctgctctatg ccgtggcctt cgcggccctg tcgctggcgc tggcctggct
     1141 gtcggtctct ggcgcgggca gctatggcgt cgccggcttc ggccggacga cggccgggct
     1201 gatcaacctc gtcctgctca tcgtgccgct gatggggctg acgctggggg cgatgagcct
     1261 ggccggcgag cgcgaaaagg gcacgctggt ctacctgctg gcccagccca tcagcgcggc
     1321 cgagctgctg ctgggcaaat tcatcggcct ggcgctggcc ctgacggcgg cgctggtcat
     1381 cggctttggc ctgaccgggc tgatgatggt tgtcagcggc agcggcggcg atttcatcat
     1441 cttcgccacg ctgatggcgc tggccatttt gctggccgtg gccagcctga gcgttggctt
     1501 cctcatctcg gccgcggtgc ggcgggcggc gacggccgtg gggctggcgc tgttcctgtg
     1561 gctggtgctg gtctattttg gcgacctggg gctgatgggc acggcgatgg tggtgcagat
     1621 ggacgtgggc caactgctga cgctggcgct ggtcaacccg ctgcaagtgt tcaagatcgc
     1681 cgccgtgcta aacctgcgcg acaacctgga ggtgctgggg ccggcgggca tctacgcctt
     1741 tcgcacctat ggcggggcat tgtggcccat gctg
//
LOCUS       DAOSMQ010000593         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_66987_length_1773_cov_3.552386, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000593 DAOSMQ010000000
VERSION     DAOSMQ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66987_length_1773_cov_3.552386"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PK829_14530"
     CDS             complement(<1..338)
                     /locus_tag="PK829_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42481.1"
                     /translation="MATQKKSTNGARKVSATSKTKSAAKKPAAKKAPAKPHRAAKPKE
                     ASPRLDDNQRSLLAGIIILFLTAVLTLSLLSPNQGQFTVWLSGVAGLVVGVGRYAIPL
                     LSGAIGGYLVL"
     gene            complement(509..1693)
                     /locus_tag="PK829_14535"
     CDS             complement(509..1693)
                     /locus_tag="PK829_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HOU42482.1"
                     /translation="MNNAQPIPHRRASRWLLALLPLALLVLLVAAFLALDPLRFFQGA
                     FPPLEELSVQRVVFPDADTIRLEVINSGPEPVTIAQVAIDNAYWNYTIHPDNTLGRLE
                     TATIDIPYRWVAGEPLPINLITSTGLTFPTAVDVAVESASFSGRTLLAYALLGIYVGI
                     IPVALGMLWYPFMRRVDRKWMNAVLALTIGLLVFLFVDTILEALELAGEAPGVFQGVP
                     VVIFGALLSLGVLLAIGRRSGEQTPMRVAALIALGIGLHNLGEGLAIGAAFATGAAAL
                     GSFLVVGFTLHNITEGVGIVAPLTRREQPPLRVFLLLALLAGGPAIAGTWIGGLAFSP
                     FVAAVFLSLGAGAILQVIWEVGRLLARDARRDGGAALTWLNFGGAALGVLIMYLTAFL
                     VK"
BASE COUNT          337 a          588 c          593 g          255 t
ORIGIN      
        1 aggacgagat agccgccgat ggccccgctg agcaggggga tggcgtagcg gccgacgccg
       61 acgaccagcc cggccacgcc cgacagccag acggtgaatt gcccctggtt gggcgacagc
      121 aggctgaggg tcaggacggc ggttaggaag aggatgatga tgcccgccag caggctgcgc
      181 tggttgtcgt ccagccgcgg cgaagcttcc ttcggcttag cggcgcgatg gggtttggcc
      241 ggggccttct tggccgccgg cttcttggcc gctgatttgg tcttgctggt ggcgctaacc
      301 ttgcgcgcgc cgttggtgct cttcttctgg gtcgccatcg ctcaaatgtt cctatgagag
      361 tatagcatgg gatgggcgat aggggccaga ggccacagga agagcagggc tttcccaatt
      421 gacctgacag atgagcgaca gggcttgcgg ccgcccacct gtcaggtcgc tccgcctgct
      481 cagcgaccta ttccattccg ccccaccgct acttcaccag gaacgccgtc aaatacataa
      541 tcagcacccc cagggccgcg ccgccgaagt tgagccacgt cagagccgcg ccgccgtcgc
      601 gccgggcgtc gcgggccaac agccggccta cttcccagat gacttgcaga atcgcccccg
      661 cccccaggct caggaagact gcggccacga acggcgagaa ggccagccca ccgatccacg
      721 tgccggcaat ggccggcccg ccggccagca gcgccagcag cagaaagacg cgcagcggcg
      781 gctgctcccg ccgcgtcagc ggggcgacga tgccgacgcc ctcggtgatg ttgtgaagcg
      841 tgaagccgac caccagaaac gaccccagcg ccgccgcgcc cgtggcgaag gccgcgccga
      901 tagccagccc ctcgcccagg ttgtgcaggc cgatgcccag cgcgatcagc gcggccacgc
      961 gcatgggcgt ctgctcgccc gaacgacggc cgatggccag cagcactccc aggctcagca
     1021 gcgcgccgaa gatgaccacc ggcacaccct ggaaaacccc cggcgcttcc ccggccagct
     1081 ccagcgcctc caggattgta tcgacgaaca ggaagacgag cagaccgatc gtcagggcca
     1141 ggacggcatt catccacttg cggtcgacgc ggcgcatgaa cgggtaccac agcattccca
     1201 gggccacagg gataatgccg acgtagatgc ccagcagcgc ataggccagc agcgtccgcc
     1261 cgctaaatga ggccgattcg acggctacat cgaccgccgt cgggaaggtc aggccggttg
     1321 aggtgatcag gttgatgggc agcggctcgc cggccaccca gcgatagggg atgtcgatgg
     1381 tggccgtctc caggcggccg agcgtattgt cggggtggat ggtgtagttc cagtaggcgt
     1441 tgtcgatggc cacctgagcg atggtcaccg gctccggccc gctattgatg acctccagac
     1501 gaatggtgtc cgcgtccgga aagacaacgc gctgcaccga cagctcctcc agcggcggga
     1561 acgcgccctg gaagaagcgc agcgggtcga gcgccaggaa agcagcgacc agcagaacca
     1621 gcagggccag cggcaagagc gccagcagcc agcgactggc gcggcggtgg ggaatgggct
     1681 gagcgttatt catgatcggg tttccttagg ccgtgacatt gaagaagccc atccagccca
     1741 actcggccag ctccgtctga tgggcgtgga aca
//
LOCUS       DAOSMQ010000594         1766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_67354_length_1766_cov_3.672122, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000594 DAOSMQ010000000
VERSION     DAOSMQ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67354_length_1766_cov_3.672122"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1766
                     /locus_tag="PK829_14540"
     CDS             <1..>1766
                     /locus_tag="PK829_14540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU42483.1"
                     /translation="PVAAAVVAPLPEEEFALPSAPVPVAAPSPASPAGLRAPVAVPAR
                     PRPVVVLPEGAALRSVVRALVAGGGLLLALSLWAGRSIPSGQRLADFVLTLIGGLALL
                     LGLQTWTRGQLASWFGRLLVRLAALLRVTPAQAVLLLLALPFAWLTRQASGNGLLAYS
                     ATVAIFAWLVAIGLAVAGSVTPRAERPAEVDRPVLGLDRWDIVLSVVLFILALALRAY
                     ATDRYPTTYSGDEGSAGLYAVELLTGKANNLFGLGWFSFPTLYFTVQSAAIALFGQTV
                     EAVRLTSAVAGALTVVALYWLGRAMFNRTTALLAALYLAASHFHIHMSRIALNNVWDG
                     LFGTLAILGLWYGWKTGRRLGFILCGLALGLGQYFYISIRPLPILFLIWAAVAFWRQR
                     PLFRERFHGLVLATVIALVAFLPLGLYFVDKPDDFQAPMNRVTIFGPWLEAELARGER
                     TTAEIILDNAINGVLGFTHEPLRLLYNPGSPLLLAGAALLFLLGILWALLNFDLRYLL
                     LLLPLLAVVASNAVSQGAPASQRYVLAMPLVALFVAVPLGEAVLWLRGQYPRYRTAVV
                     VAAFALLALVAAVDIDYYFNRV"
BASE COUNT          192 a          614 c          585 g          375 t
ORIGIN      
        1 cctgtggcgg ccgcggtggt cgcgccgttg ccggaggagg agtttgccct tccgtctgct
       61 cccgtgcctg tcgccgcccc atcgcccgcg tcaccggccg ggttgcgcgc gccggtggct
      121 gtgccggccc ggccgcggcc agttgtcgtt ctgccagagg gggcggcgct gcgctcggtg
      181 gtgagggcgt tggtcgcggg aggcgggctg ttgctggcgc tcagcctgtg ggcagggcgc
      241 tcaattcctt ccggccagcg cttggcggac tttgtactga ccctgatcgg cgggctggcc
      301 ctgctgctgg gcctacagac gtggacccgc ggccaactgg caagctggtt cggccggctg
      361 ctggtccggc tggccgccct gttgcgcgtc acaccggccc aggccgtgct gctcttgctg
      421 gccctgccct tcgcctggct gacgcggcag gcatcgggca atggcctgct ggcctatagc
      481 gctacggtgg ccatcttcgc ctggctggtg gccattggcc tggccgtggc cggcagcgtg
      541 acgcccaggg ctgagcggcc ggccgaggtg gaccggcccg tgctgggtct cgaccgttgg
      601 gacatcgtcc tctccgtggt cctgttcatt ctcgccctgg ccctgcgcgc ctacgccacc
      661 gatcgctacc cgaccaccta ctccggcgac gaaggctcgg ccggcctata tgcggtcgaa
      721 ctgttgaccg gcaaggccaa caacctgttt ggcctgggct ggttctcctt cccgacgctc
      781 tacttcaccg tccagagcgc ggctatcgcc ctattcgggc agacagtgga ggctgtccgg
      841 ctgacctcgg ccgtggctgg ggcgctgacg gtcgtggctc tctactggct cggccgggcc
      901 atgttcaacc gcacaacggc tctgctggcg gcgctctacc tggccgcctc ccacttccac
      961 atccacatga gccgcatcgc cctaaacaac gtctgggatg ggctgttcgg cacactggcc
     1021 atcctcgggc tgtggtatgg ctggaagacg ggccggcgtt taggcttcat cctctgcggg
     1081 ctggcgctgg ggctggggca gtacttttac attagcatcc gccctctgcc catcctgttc
     1141 ctcatctggg ccgcggtcgc cttctggcgt caacgaccgc tgttccgcga gcgcttccac
     1201 ggcctcgtgc tggcgacggt cattgcgttg gtcgccttcc tgccgttggg gctgtatttc
     1261 gtcgataagc ccgatgattt ccaggccccc atgaaccgcg tgaccatctt cggcccgtgg
     1321 ctggaggccg agctggcgcg cggcgagcgc acgacggccg agattatcct cgacaacgcc
     1381 atcaacggcg tgttgggctt cacccacgag ccattgcgcc tgctctacaa tcccggctca
     1441 ccgctgctac tggccggggc ggcgctgctg ttcctgctgg gcatcctctg ggcgctgctc
     1501 aactttgacc tgcgctattt gctgttgctt ctgccactgc tggccgtggt ggcctccaat
     1561 gccgtcagcc agggcgcgcc cgcctcgcag cgctatgtgt tggccatgcc gttggtggct
     1621 ctgttcgtcg ccgtgccgct gggagaggct gtcctgtggc tgcgcgggca gtatccgcgc
     1681 taccggacag ccgtcgtcgt cgccgctttt gccctgctgg ccctggtggc cgccgtcgat
     1741 atcgactact acttcaaccg cgtcta
//
LOCUS       DAOSMQ010000595         1761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_67612_length_1761_cov_4.143025, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000595 DAOSMQ010000000
VERSION     DAOSMQ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67612_length_1761_cov_4.143025"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PK829_14545"
     CDS             complement(<1..328)
                     /locus_tag="PK829_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42484.1"
                     /translation="MNRQVRKGTVLMTALGTFSMGVAAVSAQQTPTPIADTAAQTGGP
                     LGLSWWLWIVLALLLLFLLWWLFLRRKPVPPPPPDPMRRSATYERPVPPAAPMTSVTP
                     VAPPVPA"
     gene            complement(488..>1761)
                     /locus_tag="PK829_14550"
     CDS             complement(488..>1761)
                     /locus_tag="PK829_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOU42485.1"
                     /translation="MEFFNSADLSGEVVARQEMNQAEMMWFLDVLPGVDPWKFSARLT
                     ARYTAEADGEHLFSLVSAGLSRLYLDDALLVDNWSDWKPGDSFFGFGSNEIIGAAELE
                     AGRAYDVRVEYAFPAPGGMFMKALRVGVTRPLGDEAIESALIAAVQADVAILFVGQNG
                     EWDTEGRDRPHIDLVGRQNELIERVAAANPNTVVVVQSGGPVRMPWLDRVAAVLQAWY
                     PGQECGNAIADVLTGRVNPSGRLPQTFPLRLEDNPAYVNYPGENGHVLYGEGLFVGYR
                     YYDKKRVAPLFPFGHGLSYTTFAWDNLRLSSNTLAPGETLTVSIDVTNTGERAGAEVV
                     QLYVGDPQAALARPEKELKGFAKVRLAPGERRTVSLPLDMRALAYYDDAKTAWVAEAG
                     AFEVLVGASSADIRARVPFTLTADWAEATTH"
BASE COUNT          293 a          566 c          607 g          295 t
ORIGIN      
        1 gcgctggcac gggcggcgcg acaggcgtga ccgacgtcat tggggcggca ggcgggacgg
       61 gccgctcgta ggtggcgctg cggcgcatcg ggtccggcgg cggcgggggg acgggcttgc
      121 ggcgcaggaa tagccaccac agcaagaaca acagtagcag ggccagcacg atccacagcc
      181 accacgacaa accgagcggg ccgccggttt gcgcggcagt atcggctatg ggggtgggcg
      241 tctgctgggc actcacagcg gccacgccca tgctgaatgt gcccagcgcc gtcatcagca
      301 cagttccctt tcttacttga cggttcatct ttcccttcct gcttatacgc gtctccattc
      361 ggagcgcgag cgatagacct gctgctactc taaaagagga tgggggcgat gtcaagggtg
      421 gggcagaaac gcagaaacgc attcagaggg cagatcggcg tgtctgcccc tacccttgat
      481 gccgtggtta gtgggtggtc gcttcagccc aatcggccgt cagcgtgaac gggacgcggg
      541 cgcgaatgtc ggccgacgac gcgccgacca gcacctcgaa cgcgcccgcc tcggccaccc
      601 aggcggtctt ggcgtcgtcg tagtaggcca gggcgcgcat gtccagcggc aggctgactg
      661 tgcggcgctc gcccggcgcc agccttacct tggcaaagcc ctttaactcc ttctctggcc
      721 gggccagggc cgcctgcggg tcgcccacgt agagctgcac gacttccgcg ccagcacgct
      781 cgccagtgtt ggtcacgtcg atgctgaccg tcagcgtctc gcccggcgcg agggtgttgc
      841 tgctcaggcg caggttgtcc caggcgaagg tggtgtacga caggccgtgg ccgaacggga
      901 agagcggggc aacgcgcttc ttgtcgtagt agcggtagcc gacgaagagg ccctcgccgt
      961 agaggacatg gccgttttcg ccaggatagt tgacataagc ggggttgtcc tccagccgca
     1021 gtgggaacgt ctgcggcagc cggccgctgg ggttaacccg accggtcagc acgtcggcaa
     1081 tggcgttgcc gcactcctga ccgggatacc atgcttgtag cacagcggcg acacggtcga
     1141 gccagggcat gcgcactggc ccgccactct gcacgacgac aaccgtgttg gggttggccg
     1201 cggcgacgcg ctcgatgagt tcgttctgcc ggccgaccag gtcgatgtgc ggccgatcgc
     1261 gtccctcggt gtcccattcg ccgttctggc ccacaaacag gatggccacg tcggcctgca
     1321 ccgcggctat cagggcgctc tcgatggcct cgtcgcctag agggcgggtg acgccgacgc
     1381 gcagagcctt catgaacatg ccgccggggg cggggaaggc gtactcgacg cgcacgtcgt
     1441 aggcgcggcc ggcttctaac tcggccgcgc cgatgatctc gttgctgcca aagccgaaga
     1501 agctgtcgcc gggcttccag tcgctccaat tgtctaccag cagggcgtcg tcgaggtaaa
     1561 ggcggctcag cccggcgctg accaggctaa acaggtgctc gccatcggcc tcggccgtgt
     1621 agcgggccgt caggcgggcg ctgaacttcc acggatcgac accgggcagc acgtccagga
     1681 accacatcat ttcggcctgg ttcatctctt gccgggcgac aacctcgccg ctgaggtcgg
     1741 cggagttgaa gaactccatg g
//
LOCUS       DAOSMQ010000596         1757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_67809_length_1757_cov_4.960635, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000596 DAOSMQ010000000
VERSION     DAOSMQ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67809_length_1757_cov_4.960635"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1515)
                     /locus_tag="PK829_14555"
     CDS             complement(118..1515)
                     /locus_tag="PK829_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU42486.1"
                     /translation="MQGSVVTLDRETVGRFAAGLQGEVITPADAGYDEARTIYNAMID
                     KRPALIARCANAADVAAAVAFARHNKLDVAIRGGGHNGAGLALVDDGLVIDLSPMKAI
                     AVDPAARTVRVAGGCTWGEVDQATHAHGLAVPSGIISTTGVGGLTLGGGLGYLSRAYG
                     LTIDNLLAVDIVLADGRLVTASEDENADLFWAVRGGGGNFGVVTSFLFQAQPVGMVYG
                     GPIIWPIDQTEMILRWYLDFIADAPENISGWFGVHRVPTGSPLPAELHSAHGCVITWC
                     YTGPLEEAEEVFRPIRALGTPVLDLAGPLPFPQLQSMFDQIYYPPGLQWYWKADFVKE
                     VSDDAIALHLKFGSNLPSELCTMHLYPINGAVQRVGRTATAFSYRDVTFAQVIVGVES
                     DPVNVDKVSQWARQYWAALHPHSAGGAYVNMMMEEGQERVEAAYRDNYRRLATVKNKY
                     DPGNLFHVNQNIRPE"
BASE COUNT          308 a          538 c          592 g          319 t
ORIGIN      
        1 actgttagta atgtgctagc agttttttca atagagatgt ctttgcagcc gacaattagg
       61 ggctaggggt tagggaagag cgctcttcac taacccctaa cccctatccc ctatccccta
      121 ctcgggccgg atgttctggt tgacgtggaa caggttgccg ggatcgtatt tgttcttcac
      181 ggtcgccagg cggcgatagt tgtcgcgata ggcggcctcg acccgctcct ggccctcttc
      241 catcatcatg ttcacgtagg ccccgccggc cgaatggggg tggagcgcgg cccagtactg
      301 cctggcccac tggctgacct tgtcgacgtt gaccgggtcg ctctcgacgc cgacgatgac
      361 ctgcgcgaag gtgacgtcgc ggtagctgaa agccgtggcc gtgcggccga cgcgctgcac
      421 cgcgccgttg atgggataga ggtgcatggt gcacagctcc gagggcaggt tcgacccgaa
      481 tttgagatgc agggcgatgg cgtcgtcgct gacctccttg acgaagtcgg ccttccagta
      541 ccactgcagg ccgggcggat agtaaatctg gtcgaacatg ctctggagtt gcgggaaggg
      601 cagcggcccg gccaggtcaa gcactggcgt gcccagggcg cgaatggggc gaaagacttc
      661 ttcggcctct tccagcggcc ctgtgtagca ccaggtgatc acgcagccgt gggcgctatg
      721 cagctcggcc ggcaggggcg acccggtggg cacgcggtgg acgccgaacc agccggagat
      781 gttctccggc gcgtcggcga tgaagtccag ataccagcgc aggatcatct cggtctggtc
      841 aatgggccag atgatcgggc cgccatagac catgccgaca ggctgggcct ggaacaggaa
      901 ggacgtgacg acgccgaagt tgccgccgcc gccgcgcacg gcccagaaga gatcagcgtt
      961 ctcgtcctcg ctggcggtca cgagccgccc atcggccaga acgatatcga cggccagcag
     1021 attgtcgatc gtcaggccat aggcgcgcga caggtagccc agcccaccgc ccaacgtcag
     1081 gccgccgacg ccggtggtgg agatgatgcc gctgggcacg gccaggccgt gggcgtgcgt
     1141 cgcctgatcg acctcgcccc aggtgcagcc gccggccacg cgcacggtgc gcgccgcggg
     1201 atcgacggcg atggccttca tgggcgagag atcgatgacc agcccgtcat cgaccagcgc
     1261 caatccggcc ccattatggc cgccgccgcg aatggccacg tccagcttgt tgtggcgggc
     1321 aaaggccacg gccgcggcca cgtcggcggc attggcgcag cgggcgatca gcgccgggcg
     1381 cttgtcgatc atagcgttgt agatagtgcg ggcctcatcg tagcccgcgt cggcgggggt
     1441 aatcacttcg ccctgaagtc cggccgcaaa acggccgacg gtttccctgt ccagtgtcac
     1501 gactgatcct tgcatcatgg ttgatctgtc tcctaagtag cctggggcgc gcggccagtg
     1561 gcaatcgcac tgccggcgtc agcaaaccta taggagaaca gtgtaggcgg ggcggccggc
     1621 gctgtcatga gccggatgtc atcaattgtg ggcgagaagt ggggagtggc gtgggacagg
     1681 gagcctagga ggcttattcc cttcctggtg tttcatcgcc ctttaggcgt tgcaacacca
     1741 tctgtccgtc atcataa
//
LOCUS       DAOSMQ010000597         1738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_68952_length_1738_cov_3.338681, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000597 DAOSMQ010000000
VERSION     DAOSMQ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68952_length_1738_cov_3.338681"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..>1738)
                     /locus_tag="PK829_14560"
     CDS             complement(74..>1738)
                     /locus_tag="PK829_14560"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOU42487.1"
                     /translation="PNHWRGEIGPNFSNAQWNTWFAAYQAMITHYAALAAAEGVEQFA
                     VGTELNTTVSHEANWRAVIAAVRAEFPGALTYAGDWTNAPDVPWWDALDLIGVDAYYP
                     LAAAGNNTPTKAQLVAAWQPLLADLAGLSAANGGQRILFTEVGYRSQNGAAQHPWDWQ
                     SGGAVDLAEQALLYQVAFEQVFDEPWFAGAYWWAWDPEPYQGGVCDNGYTVHDKPAED
                     IVRQWYGAPPRDTTPPPPPDDSVTRLIYGDALAAGWENWSWGTTVNFNQTAQVAAGAK
                     AILADAGSWSALSLYHAPINTTAYDYLSFYVRQAAATTALHVTVNDGDDNALLDVPAA
                     DCRYTGGAPLPANTWRRVLIPLSDLGAAGAAISRVTFLNTGDGSLRFWLDDVRLVAAA
                     GDTYLPIVLTPTDDATVQSSAPKSKYGGKPTLRVRDAAADMVSYLKFDVTGLGGPPAR
                     ATLRLYASDGGPSGGAVFVVSNNNKGSSTPWREAGLTWRNAPPLNGAPLATAGAAVAG
                     QWLAVDVTAAIAGNGPVSFALRGANADLVVYSSSEGAQPPELVLEE"
BASE COUNT          214 a          574 c          678 g          272 t
ORIGIN      
        1 gggctagggg ccaggggaag aacgctcttc cctagcccct aatccctaat ccctaccccc
       61 tagcccctaa tccctactct tccagcacca actccggcgg ctgcgcccct tcgctgctgc
      121 tgtagacgac caggtcggcg ttcgcgccgc gcagggcgaa gctgaccggg ccgttgccgg
      181 cgatggccgc ggtcacgtcc accgccagcc attgcccggc gacggccgcg ccggccgtgg
      241 ccagcggcgc gccgttgagg ggcggggcgt tgcgccaggt caggccggcc tcgcgccagg
      301 gggtgctgct gcccttgttg ttgttgctga cgacgaagac cgccccgccg gacggcccgc
      361 cgtcgctggc gtagagccgc agcgtggccc gcgccggcgg cccgcccagc ccggtcacgt
      421 cgaacttgag gtagctgacc atgtcggcgg cggcgtcgcg gacgcgcagc gtgggcttgc
      481 cgccgtactt gctcttgggg gccgacgatt gcacggtggc gtcgtcggtc ggcgtcagga
      541 cgatgggcag gtaggtgtcg ccggccgcgg ccaccaggcg gacgtcgtcg agccagaagc
      601 gcagggagcc gtcgccggtg ttgaggaagg tcacgcggct gatggccgcg ccggccgcgc
      661 cgaggtcgct gagcgggatg aggacgcgcc gccaggtgtt ggccggcagg ggcgcgccgc
      721 cggtgtagcg gcagtcggcc gcgggcacgt ccagcagcgc gttgtcgtcg ccgtcgttga
      781 cggtcacgtg cagcgccgtc gtcgccgccg cctggcggac gtagaaggac aggtagtcat
      841 aggcggtcgt gttgatgggc gcgtggtaga gcgacagggc gctccagctg cccgcgtcgg
      901 ccaggatggc tttggcgccg gcggcgacct gggcggtctg gttgaagttg accgtcgtcc
      961 cccatgacca gttctcccag ccggcggcca gggcgtcgcc atagatgagc cgggtcacgc
     1021 tgtcgtcggg cggcggtggg ggcgtcgtgt cgcgcggcgg cgcgccatac cactggcgga
     1081 cgatgtcctc ggccggcttg tcgtggacgg tgtagccgtt gtcgcagacg ccgccctggt
     1141 acggctccgg gtcccaggcc caccagtagg ccccggcgaa ccacggctcg tcgaagacct
     1201 gctcgaaggc gacctggtag aggagcgcct gctcggccag atcgaccgcg ccgccgctct
     1261 gccagtccca ggggtgttgg gccgcgccgt tctggctgcg atagccgacc tcggtgaaga
     1321 ggatgcgctg gccgccgttg gcggcgctga ggccggccag gtcggccagc agcggctgcc
     1381 aggcggcgac gagttgggcc ttggtgggcg tgttgttgcc cgcggcggcc agcgggtagt
     1441 aggcatcgac gccgatgagg tcgagcgcgt cccaccaggg cacgtcgggg gcgttggtcc
     1501 agtcgccagc gtaggtcagc gcgccgggga actcggcgcg gacggcggcg atgacggcgc
     1561 gccagttggc ttcgtggctg acggtggtgt tgagttccgt gcccacggcg aactgctcca
     1621 cgccctcggc cgcggccagc gcggcgtagt gggtgatcat ggcctgatag gcggcgaacc
     1681 aggtgttcca ctgggcattg ctgaagttgg ggccgatttc gccgcgccag tggttggg
//
LOCUS       DAOSMQ010000598         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_69159_length_1734_cov_4.369863, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000598 DAOSMQ010000000
VERSION     DAOSMQ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69159_length_1734_cov_4.369863"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PK829_14565"
     CDS             <1..563
                     /locus_tag="PK829_14565"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOU42488.1"
                     /translation="IHPDRAVDLWNALLAAGDSYGLKACGLAARDSTRTEAGLPLYGH
                     EMAGALGLNPADAGFGSFVKVYKPFFIGRDAFVRHEATRDRDLVRFRMNEKGVRRPES
                     GDPVLDRRGKVIGHVTSCAIDREGYLLGQAIVPSAMAEPGTPLFIYQMGGGTRPIKGV
                     ERADLGGRLPMPDAATVQTRFPERKK"
     gene            677..1177
                     /locus_tag="PK829_14570"
     CDS             677..1177
                     /locus_tag="PK829_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOU42489.1"
                     /translation="MRREVLTWSDVDKLIDYLVPQIRGRFDSMVIITRGGIVPGGMLA
                     EALDITYILTASVRFPSDFPGMQPEMVKYAIPEFIQFPDDDVLRGRRVLVVDDVWTKG
                     RNSVTVANRIDSAGGTPEICVLHYKPSYSLYPGYAPTYYAAVTDAYMIYPWELDRGPE
                     MLGAWN"
     gene            1288..1386
                     /gene="ffs"
                     /locus_tag="PK829_14575"
     ncRNA           1288..1386
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK829_14575"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1423..>1734
                     /locus_tag="PK829_14580"
     CDS             1423..>1734
                     /locus_tag="PK829_14580"
                     /inference="COORDINATES: protein motif:HMM:NF015923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HOU42490.1"
                     /translation="MTRSPLRRTSRGGAPVASYQPRWPTSPRRQAWFFLCLLVVLVAA
                     GCRPAALTYTVRDDAAAVTVSGRYATVGEVVAAAGLALGQGDRLSPAAGAPADPATPI
                     AI"
BASE COUNT          288 a          592 c          572 g          282 t
ORIGIN      
        1 tcatccaccc cgaccgcgcg gtagacctct ggaacgcgct gctggccgcg ggcgacagct
       61 atggcctgaa ggcctgcggc ctggcggcac gcgacagcac gcgcaccgag gcgggcctgc
      121 cgctctatgg ccatgagatg gccggggcgc tggggttgaa cccggccgac gccggcttcg
      181 gcagcttcgt caaagtctac aagccgtttt tcatcggccg cgacgccttt gtgcggcatg
      241 aggccacccg cgaccgcgac ctggtgcgct tccgcatgaa cgagaaaggc gtgcgccggc
      301 cagagtcagg cgacccggtg ctcgaccggc gcggcaaggt catcggccac gttaccagct
      361 gcgccatcga ccgcgagggt tacctgctgg gccaggcaat tgtaccttcg gcgatggccg
      421 aaccgggcac gccgctattc atctaccaga tgggcggcgg cacacggccg atcaagggcg
      481 tggagcgtgc cgacctgggc ggacgcctgc ccatgcccga cgcggccacg gtgcagacgc
      541 gcttcccgga gcgcaagaag taagaaatga caggggcacg gccggcggtg gggcgatacg
      601 ccgccggcca tgcgtcaatt gacttgcccc aaccaacagg gtaggatcac gcatcagcaa
      661 cgacgaggag tggacaatgc ggcgcgaagt gctcacgtgg tctgacgtcg acaaactcat
      721 cgactacctg gttccccaga ttcgagggcg gttcgactcc atggtaatca tcactcgtgg
      781 cgggattgtg cccggcggga tgctggcgga ggcgctggac atcacctata tcctgaccgc
      841 ctcggtgcgc tttccgtccg acttccccgg gatgcagccg gagatggtca agtacgccat
      901 cccagagttc atccagttcc ccgacgacga cgtgctgcgc gggcggcggg tgctggtggt
      961 cgacgacgtc tggactaagg ggcgcaacag cgtcaccgtg gccaaccgca tcgactctgc
     1021 cggcggcacg ccggagatct gcgtgctaca ctacaagccg tcctattcgc tttaccccgg
     1081 ctacgcgccg acctactacg ccgccgtaac cgacgcttac atgatctatc cgtgggagct
     1141 ggaccgcggc ccggagatgc tgggggcctg gaattaaccg gccgattgtg tgtaatcact
     1201 gaggcccgcc cgcccgccgt tgtagaacac gcgcgtctgg gctatcatta ggcccgttaa
     1261 caggaatgtg cccatgaggc tggtgaccac tgggtccctg gtggcggaag ccgttgaacc
     1321 gtgtcaggtc cggaaggaag cagcactaag acgtttcttc cgggtaccca ggacaacctg
     1381 gtcggtcacc ggcctcatgg gcacattcgc gttgaaggcc gaatgactcg cagcccactc
     1441 cggcgcacgt cccgcggcgg cgcgcccgtc gcgtcctatc agcctcgttg gccaacaagc
     1501 ccgcgccggc aagcgtggtt ctttttgtgc ctgctggtcg tgctggtggc cgccggctgc
     1561 cgccccgcgg cgctaaccta caccgtgcgc gacgatgcgg cggccgtgac ggtcagcggc
     1621 cgctacgcca ccgtcggcga ggtcgtcgcc gccgccgggc tggccctggg ccagggcgac
     1681 cgcctctctc ccgccgccgg cgctccggcc gacccggcca cgcccatcgc catc
//
LOCUS       DAOSMQ010000599         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_69317_length_1731_cov_4.452864, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000599 DAOSMQ010000000
VERSION     DAOSMQ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69317_length_1731_cov_4.452864"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PK829_14585"
     CDS             complement(<1..458)
                     /locus_tag="PK829_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOU42491.1"
                     /translation="MNTVRAIPAAETRVELVVVNSRFLATAAPAFSVEAARAFVGRVR
                     AEFADATHNVPAYLIGHGAATIAHCHDDGEPSGTAGRPVLAVLQGSGLGDIAVVVTRY
                     FGGTKLGTGGLVRAYGDAARAVVAALPRAEKVATVTALIEVPYSLLERLRL"
     gene            799..1650
                     /locus_tag="PK829_14590"
     CDS             799..1650
                     /locus_tag="PK829_14590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HOU42492.1"
                     /translation="MTVLLLTINYSLMILVPATLAVFIWRRTGAPWRFFFMGAVTFVL
                     SQVLHIPFNAVVQRSGLLPATTATAGLLITALFLGLSSGVFEEVARYLTYRFWARDAR
                     TWSRGLMLGAGHGGSEAIILGVLGAVSTFSLLAVANNEVAMNALPAEQRQVVADALAQ
                     VVNMPAPMLILGAVERVFAIACHLAMSLLVLQVFLRRNIGWLFVSIGFHAALNMVSVI
                     AVSRVGPYWTEVVVGLFALLAVVIVFRLRGPEPAEPEPTPLPPPVDADALSLQPSDDA
                     LDRSRYS"
BASE COUNT          265 a          595 c          548 g          323 t
ORIGIN      
        1 agccgcagac gttccagcaa cgaatagggc acttcgatca gagccgtcac tgtggctacc
       61 ttctcggcgc gcggcagggc ggcgacaacc gcccgcgccg cgtcgccata ggcccgcacc
      121 agcccgcccg tgcccagctt cgtgccgccg aagtagcgcg tcacaacgac ggcgatgtcg
      181 cccaggccgc tgccctgtaa gacggccagc accggccgcc cggcggtgcc cgacggctcg
      241 ccgtcatcgt ggcagtgggc aatggtggct gcgccgtggc cgatgaggta ggcggggacg
      301 ttgtgcgtgg cgtcggcgaa ctcagcccgc acgcggccga cgaacgctcg cgccgcctcg
      361 acgctgaagg caggcgcggc cgtggcgagg aagcgcgagt taacaacgac caactcgacg
      421 cgcgtctcgg ccgcggggat ggctcgcacg gtgttcacca cgctattgta gaccggttgc
      481 agcggttgtc atgccggcgc gctggcgcgc tccggcgctc cggccggcaa ctgaaaaccc
      541 ccactgtatg acatttgtca cctttacgag cgccccctat ttatgctatg atgtttgtga
      601 agccaccgca acggcagagc gtcgtgagtc aaactatttg agttacgaca ggcaaacttg
      661 ggctaggcag ccccgtgggg ttgccccagt tgccgatact gagtggggat tgtctgcact
      721 ccacacgctt gtctccgcgg tggccttctt cttgccgggc gagtccgttg ctccatgccc
      781 acatcctgga aatagcccat gactgtactt ctgctgacaa tcaactacag tctcatgatt
      841 ctcgtccccg ccaccctggc cgttttcatc tggcggcgca cgggcgcgcc gtggcgcttc
      901 ttcttcatgg gcgcagtgac ctttgtgcta tcccaggtgt tgcacatccc gtttaatgcc
      961 gttgtccagc gctccggcct gctgccggcc acaacggcga cggccggcct gctcatcacc
     1021 gccctctttt tgggcctgtc atcgggcgtc ttcgaggaag tggcccgcta cctgacctac
     1081 cgcttctggg ccagggacgc ccgcacgtgg tcgcgcggcc tgatgctcgg cgcgggccac
     1141 ggcggcagtg aggcgattat cctgggcgtg ctgggagcgg tcagcacgtt ctccctactc
     1201 gccgtcgcca acaacgaggt agcgatgaac gccctgcccg cggagcagcg tcaggtcgtg
     1261 gccgacgccc tggctcaggt cgtgaacatg cccgcgccga tgttgattct gggggcggtc
     1321 gagcgcgtgt tcgccatcgc ctgccatctg gccatgtcgc tgctggtctt gcaggtcttc
     1381 ctgcggcgta acatcggctg gctctttgtg tccatcggct tccacgccgc gctgaacatg
     1441 gtcagcgtca tcgccgtgtc gcgtgtcggc ccctattgga cggaggtcgt cgttggcctg
     1501 ttcgccctgc tggcggtggt catcgtcttc cgcctgcgcg gcccggagcc ggccgagcca
     1561 gagccaaccc cgctgccgcc gccggttgac gccgatgccc tgtccctgca accaagcgac
     1621 gacgccctcg accgcagccg ctacagctaa gcgcgcggcc ggagccgcaa ccggcgcagg
     1681 gccaaagcgt tgagcagatc aaagacgaac agaaaacccc cttgcagcat g
//
LOCUS       DAOSMQ010000600         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_69369_length_1730_cov_5.204179, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000600 DAOSMQ010000000
VERSION     DAOSMQ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69369_length_1730_cov_5.204179"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PK829_14595"
     CDS             <1..1220
                     /locus_tag="PK829_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU42493.1"
                     /translation="RLSQPAGAVKFPTGVDHHQQQALLANPKATSAEVWESLYRNSPQ
                     YRRFVADRLQAAGNVSAVSHQRPRQPAKQKVSSLAQFGVLARRYFDLIRRDSGSLVIL
                     LAAMPLIGLLLLVMADRYDLTGKSEPAIRSQIQQEILDARRAGDPGNDVEQFQGIYQV
                     AGATQRLLFMMALAATLLGLFGAAYEIVKEAPIYHRERMVNLRIPPYLLSKIVILLGF
                     GLLQCLLLLLVMSLRVAFPGDGVFLPAAVEMYITLVLATLASITLGLLISAVVRSSAT
                     VIYIILVVLFVQIIFAGAIFEIPAAARPISYLTTTRWTLEALGSSVNIERLNARGGGC
                     LEPEEPLPAGLAPAAEGYCQTGQTALPPALTFNVSYDHSTGHLLASWLILLAFAAAFL
                     GLTIVVQRRKDVV"
     gene            complement(1251..>1730)
                     /locus_tag="PK829_14600"
     CDS             complement(1251..>1730)
                     /locus_tag="PK829_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42494.1"
                     /translation="VGKSGAPPITIGGYLMRRRRLGLLSDQLDEDERHRLAVANADYD
                     GIIARQTVRFEARILAEVGGRMREWMEYLRNLAVSKRLVADHAHYIWKADTRVVIGEL
                     LDAFGPTLPGHVTDDVAMLDRRLQTRWAPDAFIWAAVWQPAYPAERFWWLYGYPAAH"
BASE COUNT          275 a          600 c          542 g          313 t
ORIGIN      
        1 cccgcctcag ccagccggcc ggcgcggtca aatttcccac cggcgtagac caccaccagc
       61 agcaagctct gctggctaac cccaaggcga catcagccga ggtgtgggag tcgctctatc
      121 gcaactcgcc gcagtaccgg cgcttcgtgg ccgaccggct ccaggcggcc ggcaacgttt
      181 ccgctgtcag ccaccagcgc ccccgccaac cggccaagca aaaggtttcc tcgctggccc
      241 agttcggcgt gttggcgcgg cgctacttcg acctcatccg ccgcgacagc ggtagcctgg
      301 tcatccttct ggcagccatg cctctcatcg gtctgctgct gctggtcatg gccgaccgct
      361 atgacctgac cggcaagagc gagccggcca tccgcagtca gattcagcag gaaatcctcg
      421 acgcccgccg cgccggcgat cctggcaacg acgtcgagca attccagggc atctaccagg
      481 tggccggggc aacgcaacga ctgctcttta tgatggccct ggcggcgacg ctgctggggc
      541 tgtttggcgc ggcctatgag atcgtcaagg aggcgccgat ctaccaccgc gagcggatgg
      601 tcaacctgcg catcccgccc tacttgctgt cgaagatcgt cattctgctc ggctttgggt
      661 tgctgcaatg cctgctgctg ctactcgtca tgagcctgcg ggtggccttt ccgggcgacg
      721 gcgtctttct gccggccgcc gtggagatgt atatcaccct ggtgctggcg acgctggcca
      781 gcatcaccct gggcctgctc atctcggccg tcgtccgcag ttcagccacg gtcatctaca
      841 tcatcctggt cgtcctcttc gtgcagatca ttttcgccgg ggccatcttc gagattccgg
      901 cggcggcgcg gcccatttcc tacctgacga cgacgcgttg gacgttggaa gcgctgggca
      961 gctcggtgaa catcgagcgg ctgaacgcgc gcggcggcgg ctgcctggag ccggaggaac
     1021 cgctgccggc cgggctggcc cccgcggctg agggctattg ccagacgggc cagaccgccc
     1081 taccgccggc gctgaccttc aacgtcagct atgaccactc gaccggccac ctgctggcgt
     1141 cctggcttat cttgctggcc ttcgccgccg ccttcctggg gctgaccatc gttgtccagc
     1201 gacggaagga cgtggtctaa ccgcgttcag ggagaaactc gctttcccgg tcaatgcgcc
     1261 gccggatagc cgtagagcca ccagaagcgc tcggccgggt aggctggctg ccaaacggcg
     1321 gcccagatga atgcatccgg cgcccagcgg gtctgcaagc gccggtcgag catggccacg
     1381 tcgtcagtga catggccggg cagggtgggg ccgaacgcat cgagcagttc cccaatgacg
     1441 acgcgggtgt ccgccttcca gatgtagtgg gcatgatcgg ccaccaggcg cttcgacaca
     1501 gctagattgc gcaggtactc catccattcg cgcatgcgtc cgccgacctc ggccagtatt
     1561 cgcgcctcga agcgaaccgt ctggcgggcg ataatcccat cgtagtcggc attggccacc
     1621 gccagccgat ggcgctcgtc ctcgtctagc tggtcgctca acaggcccag gcggcgacga
     1681 cgcatcagat agccgccgat ggtgatgggc ggcgcgccgc tcttgcccac
//
LOCUS       DAOSMQ010000601         1714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_70226_length_1714_cov_5.890295, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000601 DAOSMQ010000000
VERSION     DAOSMQ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70226_length_1714_cov_5.890295"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK829_14605"
     CDS             complement(<1..228)
                     /locus_tag="PK829_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42495.1"
                     /translation="MNPAPLAGYAAPPLRVCIFGTYRAGYTRNQIILKGLQAQPDVAV
                     HVCHATLWQGIEYREPKASRQWRKPRLWRRLA"
     gene            274..1137
                     /locus_tag="PK829_14610"
     CDS             274..1137
                     /locus_tag="PK829_14610"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HOU42496.1"
                     /translation="MILVTGATGFIGRSLTRVLDHAGHAWRPYTGRLSDAAALAEALD
                     GATTVIHLAGAEARGNRRLLQHVDVEGTQRLVEMARPAGVRHLIVPSRLHANVHAIHP
                     LPRAKGEVERLVARSGVPYTILRTTTLFGRDDRFTELLLSLAIWSWPFAWVPNGGAMP
                     TQPLWVEDFTHCLVQILDRPDLINRTLTVGGAERLSYRQLVQMILRVTGRQRLLLPVP
                     LKATRRIARFLLAWWHWPPASLFFLDRFSAAEVVELDAAQRIFGLQPARFGETITYLN
                     RGGMRWRLFRR"
     gene            complement(1195..>1714)
                     /gene="metW"
                     /locus_tag="PK829_14615"
     CDS             complement(1195..>1714)
                     /gene="metW"
                     /locus_tag="PK829_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOU42497.1"
                     /translation="CGDGALLDYLKREKHARGRGIELSEAGVLACVRRGLSVRQGNLQ
                     EGLADYPDGSFDCVILAQTLQYLDDPVMVLGEMLRVGRRAIVSFPNWGYWRCRLELLA
                     HGRIPAAPDLPQPWHDIPRWQALTAADFAAFCRQRSFDVSQAIYLAGERRVAALANLR
                     AQTAVFALERRS"
BASE COUNT          264 a          610 c          569 g          271 t
ORIGIN      
        1 agccagccgg cgccataagc gcggtttgcg ccactggcgg ctagccttgg gctcgcgata
       61 ctcgatgccc tgccacagcg tcgcgtggca gacatgcacg gccacgtccg gctgggcctg
      121 caagcccttg aggatgatct ggttgcgggt gtagccggcg cggtaggtgc cgaagatgca
      181 gacgcgcaac ggcggcgcgg catagccggc cagaggggct ggattcatcg tcatcattat
      241 agcagccgct aaccggcccg gtacaatagc ggcatgatcc tcgtcaccgg cgcgaccggc
      301 ttcatcggcc gctcgctcac ccgcgtcctc gaccacgccg gccacgcctg gcgaccctac
      361 accggccgtc tgagcgacgc cgcggccctg gccgaggcgc tggacggggc aacgacagtc
      421 atccatctgg ccggggccga ggcgcgcggc aaccgccgcc tgttgcaaca cgtggacgtg
      481 gaaggcacac aacggctggt ggagatggcc cggccggccg gcgtgcgcca tctcatcgtg
      541 cccagccgcc tgcacgccaa cgtccacgcc atccaccccc tgccgcgggc caaaggcgag
      601 gtcgagcggc tggtggcccg cagcggcgtc ccctacacca tcctgcgcac gacgacgctg
      661 tttggccgcg acgaccgttt caccgagctg ctgctcagcc tggccatctg gagctggccg
      721 ttcgcctggg ttcccaacgg cggcgcgatg cccacgcagc cgttgtgggt ggaagacttc
      781 acccattgtc ttgtccaaat cctcgaccgg cccgacctga tcaaccggac gctgactgtg
      841 ggcggcgcgg agcggctgag ctaccgccaa ctggtgcaga tgatcctccg cgtcaccggc
      901 cggcagcgcc ttctgctgcc tgtgcccctg aaggcgacgc ggcggatagc gcgctttctg
      961 ttggcctggt ggcattggcc gcccgccagc ctcttcttcc tggatcgctt ctccgccgcg
     1021 gaggtcgtcg aactggacgc cgcccagcgc atcttcggtc tgcaaccggc gcgcttcggc
     1081 gagacgatca cctacctcaa ccgcggcgga atgcgttggc ggctgtttag aagataaatg
     1141 aggtcaacgc ccaagcgcca ggcttcttcc tcccggcgtc ctggcggtct ggcgttaact
     1201 tctccgctcc agcgcaaaga cggccgtctg cgcccgcagg ttggccagag cggccacgcg
     1261 ccgctcccca gccaggtaga tggcctgtga cacgtcgaag ctccgttgcc ggcaaaaggc
     1321 ggcgaagtcg gccgcggtca gcgcctgcca gcgagggatg tcgtgccacg gctgcggcag
     1381 gtcgggcgcg gccgggatgc gtccgtgggc cagcaattcc agccggcagc gccaatagcc
     1441 ccagttgggg aagctgacga tggcccgccg gccgacgcgc aacatctcgc ccagcaccat
     1501 caccgggtcg tcgagatatt gcagcgtttg ggccaggatg acgcagtcga agctgccgtc
     1561 ggggtagtcg gccaggccct cttgcaggtt gccctggcgc acactgaggc cgcgccgcac
     1621 acaggccagc acgcccgcct cgctgagttc aatgccgcgg ccgcgggcgt gcttctcgcg
     1681 cttcaggtaa tcgagcagcg cgccgtcgcc gcag
//
LOCUS       DAOSMQ010000602         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_70812_length_1704_cov_4.366283, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000602 DAOSMQ010000000
VERSION     DAOSMQ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70812_length_1704_cov_4.366283"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK829_14620"
     CDS             <1..350
                     /locus_tag="PK829_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOU42498.1"
                     /translation="SIAANLARNQRRSLGRYWAAMQRAFLAAPRHHAAPPERTPANDA
                     RRLREAVGRLRPDARDVVYLRYFLGLSEAETAAALDIPPGTAKSRHSRALAQLRAIIA
                     ADYPDLREALGDD"
     gene            351..1169
                     /locus_tag="PK829_14625"
     CDS             351..1169
                     /locus_tag="PK829_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42499.1"
                     /translation="MEPTTDTHWQRALAQLAAQVEYPPTPNIAARARDGVTGRQMLPA
                     ARPARPAGRRLAWALGVMALLALSLLAVPATRAALLSFFARVGAIEIFIDESAPTVPA
                     PTRPPSTPSFSALPATTTPEAVTHSLSLIDLGEPTTLAEAERQAHFTPDLPAALGAPD
                     EVYTHPGIDLPAITLVWRPDDGPPLSLTAIAVAEFARKVVAATAVEEVAVGDETGLWL
                     TGPHHLQLFGDWQGSNRLLITSNVLIWVHGGITYRLEGDLSQADAVAIAASLGD"
     gene            1258..>1704
                     /locus_tag="PK829_14630"
     CDS             1258..>1704
                     /locus_tag="PK829_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42500.1"
                     /translation="MKRASVLVVVGLLLLATATAVLAGGWAVVTLDSPPGEIHAGEPW
                     TVGFTVLQHGQTPVHNLGPGNPVEPLLIATNADGRRVEAQATPTDEIGHFVAEVTFPT
                     EGDWEWTIHPNPLAGESLFEPLTVMAARPAAAEEVVAPAAAQPAAVA"
BASE COUNT          247 a          645 c          585 g          227 t
ORIGIN      
        1 tcagcatcgc cgccaacctg gcccgcaacc agcggcgcag cctgggccgc tactgggcgg
       61 ccatgcagcg cgcctttctg gccgcgccgc gccaccacgc cgcgccgccg gagcgcacgc
      121 cggccaacga cgcccgccgc ctgcgcgaag ccgtcggccg gctgcggccc gacgcccgcg
      181 acgtggtgta tctgcgctat ttcctgggcc tgtcggaggc ggagacggcc gcggcgctgg
      241 acatcccacc gggcacggcc aagtcgcgcc acagccgcgc gctggcccag ttgcgcgcca
      301 tcatcgccgc cgactacccc gacctgcgcg aggcgctggg agacgactaa atggaaccaa
      361 cgaccgacac ccactggcaa cgcgccctgg cccaactggc ggcgcaggtc gagtacccgc
      421 caacgcccaa catcgccgcg cgcgcccgcg atggcgttac cgggcgacag atgttgcccg
      481 ccgcgcggcc ggccaggccg gccggccgtc gcctggcctg ggcgctgggg gtgatggccc
      541 tcctggcgct gagcctgctg gccgtgccgg caacgcgggc ggcgctgctg tcgttcttcg
      601 cccgcgtcgg ggccattgag attttcatcg acgagagcgc gccgacggtg cccgcgccga
      661 cgcggccgcc gtctacgccc tccttctccg ccctgccggc cacgaccacc ccggaagccg
      721 tgacccactc gctgtcgctc atcgacctgg gcgagccgac gacgctggcc gaggccgagc
      781 ggcaggccca cttcacgccc gatctgcccg cggcgctggg cgcgcccgac gaggtctaca
      841 cccacccggg catcgacctg ccggccatca ccctcgtctg gcggccggac gacggcccgc
      901 cgctgtcgct gacggctatc gccgtggccg agttcgcccg caaggtcgtt gccgcgacgg
      961 cggtcgagga ggtggccgtg ggcgacgaga cgggtttgtg gctgaccggg ccacaccact
     1021 tgcaactatt tggcgactgg caggggtcga atcgactgct gatcacctcc aacgtgctca
     1081 tctgggtgca cggcggcatc acctaccggc tggaaggcga cctgagccag gccgacgccg
     1141 tggccatcgc cgcgtcgctg ggcgattgac ccgccggcgg gaaccggccg cgaccgggcg
     1201 gtgtactatg ggcacacggt cgaacctgac cgtcaattca aaaagggaga gtgaaagatg
     1261 aagcgcgcaa gtgttcttgt ggtggtgggg ctgctgctac tggccacggc cacggccgtg
     1321 ctggctggcg gctgggcggt ggtcacgctg gacagtccgc cgggcgagat ccacgccggc
     1381 gagccgtgga cggtgggctt caccgtcttg cagcatggcc agacgcccgt ccacaacctc
     1441 ggccccggca accccgtcga gccgctgctg attgccacca acgccgacgg ccgccgcgtc
     1501 gaggcacagg ccacgccgac cgacgaaatc ggccacttcg tcgccgaagt caccttcccc
     1561 accgagggcg actgggaatg gacaatccat cccaacccgc tggccggtga gtcgctattt
     1621 gagccgctga ccgtgatggc ggcgcggccg gccgcggccg aggaagtcgt cgccccagcc
     1681 gccgcccaac cggctgcggt ggcc
//
LOCUS       DAOSMQ010000603         1703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_70875_length_1703_cov_4.004248, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000603 DAOSMQ010000000
VERSION     DAOSMQ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70875_length_1703_cov_4.004248"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1703)
                     /gene="glmS"
                     /locus_tag="PK829_14635"
     CDS             complement(<1..>1703)
                     /gene="glmS"
                     /locus_tag="PK829_14635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOU42501.1"
                     /translation="RLEYRGYDSAGIAVIDGEAIDIRRDVGKLGNLEALLRRSPLAGH
                     LAIGHTRWATHGVPSQRNAHPHLGMEGRVVLVHNGIVENYQSLRAELSAEGVAFRSET
                     DTEVIVQMVERFMAAGLSLEDAARQTLGRLEGANAVVLMSVDQPDRLVCARIGNAGGI
                     ALGIGAGEMFIASDIPAILEYTRQIIFLESRQMAVVTVDGYRVTDLDGRPVRAEVHAI
                     AWDPVSAEKGEYKHFMQKEIFEQPRALIDTLGGRIDFDTGCVSLPEMNLTPELVARID
                     KLYIVACGTSYYAGLVGKYLIETLARLPVEVEYASEFRYYNPIINEHTAVLSITQSGE
                     TADTLAAAEQARAQGATLWAIVNAIGSQSMRLSDGAIAMHAGPEIGVASTKAFTASLA
                     DLYLLACALGTLRGTIAPGQMRQLADDVARLPALVGRALERDADYQALAERFHRASDF
                     LFLGRGINYPIALEGALKLKEISYIHAEGYPAGEMKHGPIALIDEEMPVLAIVTKDGL
                     YEKMLSQVQQAKARGGVVIALATEGDEAIAREADTVITVPEAPPLLAPIVNVVPLQLL
                     S"
BASE COUNT          269 a          568 c          580 g          286 t
ORIGIN      
        1 gctgagcagc tgcaagggca cgacgttgac gatgggggcc agcagcggcg gcgcttccgg
       61 cacagtgatg accgtatccg cctcgcgggc gatggcctca tccccctcgg tggccagggc
      121 aatgaccacg ccgccgcgcg cctttgcttg ctgcacctgg ctgagcatct tctcgtagag
      181 gccatccttc gtcacgatgg ccagcacggg catctcctcg tcgatcaggg cgatggggcc
      241 gtgcttcatc tcgcccgcgg ggtagccctc ggcgtggatg tagctgatct ccttcagctt
      301 gagcgcccct tccagggcga tggggtagtt aatgccccgc cccaggaaca ggaagtcact
      361 ggcgcggtgg aagcgctcgg ccagcgcctg gtagtcggca tcgcgctcca gcgcccggcc
      421 gaccagcgcc ggcagccggg cgacatcgtc ggccaactgc cgcatctggc ccggcgcaat
      481 cgtcccgcgc agcgtgccca gggcacaggc cagcaggtag aggtcggcca gcgaggccgt
      541 aaacgccttg gtgctggcca cgccgatctc cggcccggcg tgcatggcga tggcgccgtc
      601 ggacaggcgc atcgactggc tgccgatggc gttgacgatg gcccatagcg tcgcgccctg
      661 ggcgcgggcc tgctcggccg cggccagcgt gtcggctgtc tcgccgctct gggtgatcga
      721 taggacggcg gtgtgttcat tgatgatagg gttgtagtag cggaactccg aagcgtactc
      781 gacctccact ggcaagcgcg ccagcgtctc gatcaggtat ttacccacca acccggcgta
      841 gtagctcgtg ccgcaggcga cgatatacag cttgtcgatg cgcgccacca gctccggcgt
      901 caggttcatc tctggcagag agacacagcc cgtgtcgaag tcgatccgcc cgccgagggt
      961 gtcgatgagc gctcgcggct gctcgaagat ctctttctgc atgaagtgct tgtactcgcc
     1021 cttctcggcg ctgacggggt cccaggcgat ggcgtggact tcggcgcgca caggccggcc
     1081 gtccaggtcg gtcacgcgat agccgtccac agtgacgacg gccatctgac ggctctccag
     1141 gaagatgatt tgccgcgtgt actccaggat ggcagggatg tcggaggcga tgaacatctc
     1201 gcccgcgccg atgcccaggg cgatgccgcc ggcgttgccg atgcgggcgc agaccaggcg
     1261 gtcgggctga tcgacgctca tcaggacgac ggcattggcc ccttccaggc gacccaacgt
     1321 ctgccgggcg gcgtcctcca gcgacaggcc ggcggccatg aagcgctcga ccatctggac
     1381 aatgacttcg gtgtcggtct ccgagcggaa agcgacgccc tccgcggaca actccgcgcg
     1441 caagctctgg taattctcaa cgatgccgtt gtggacaagg acgacgcggc cctccatgcc
     1501 caggtggggg tgggcgttgc gctggctggg cacgccgtgg gtggcccagc gcgtgtggcc
     1561 gatggccaga tgcccggcca ggggcgaccg gcgcaacagc gcttccagat tgcccagctt
     1621 gcccacgtcg cggcgaatgt cgatggcctc gccgtcgatg acggcaatgc cggccgaatc
     1681 atagccgcga tactccaacc gcc
//
LOCUS       DAOSMQ010000604         1697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_71255_length_1697_cov_3.551766, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000604 DAOSMQ010000000
VERSION     DAOSMQ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71255_length_1697_cov_3.551766"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK829_14640"
     CDS             complement(<1..515)
                     /locus_tag="PK829_14640"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOU42502.1"
                     /translation="MPLTDILSAVRWWAVLTLLGLVAWPLVFTLLRPLADRGYAFVKM
                     AGLLLVSYVFWLAGSLGLADNNGGGILLALAAVGAASWLAYRRRPAGDVGLREWLRAN
                     RGAVVAAELVFLLVFALWVWVRAQNPAIANTEKPMEFAFLNGVTLSPTFPPRDPWLSG
                     YGISYYYFGYV"
     gene            complement(518..>1697)
                     /locus_tag="PK829_14645"
     CDS             complement(518..>1697)
                     /locus_tag="PK829_14645"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOU42503.1"
                     /translation="HVWNFLLVHGLFLLFVVTHLARELRAWTATWTEEGKRRLEPLGR
                     PLLLAAGLFVVLCAALLIRGYWIAPVVLPLLAASGLLTLRPGLPAARRVVLALMAAAL
                     GLTLIVEIVVLDGDIGRMNTVFKFYLQVWLMLSVACGPAAVWAWPSIRQTRRLRLVWQ
                     TVLGVMLFAALLYPLLATAAKWGIRMNPDAPTTLDGAAFMPTVEYGDTDYAGQSRTVR
                     LVEDMGAIDWLQRNVQGSPVVMEAHGSNPYRSIASRIAMYTGLPTVVGWDWHQRQQRA
                     VAPDTLVSSRINDVNTFYNTLDTNEAWNILARYGVEYIVVGSLENTYYWPEGLSKFEA
                     MVADGTLAEVYRDEFARIYRVVALQERDAQSASPAPQAANEQHPAAGQLSIDRIVGEN
                     "
BASE COUNT          321 a          596 c          542 g          238 t
ORIGIN      
        1 atgacgtagc cgaagtagta gtagctgatg ccatagcccg acaaccacgg gtcgcgcggc
       61 gggaaagttg ggctgagcgt gacgccgttg aggaaggcga actccatcgg cttctcggtg
      121 ttggcgatag ccgggttctg cgcgcgcacc cacacccaca gggcaaagac aagcagaaag
      181 accagctcgg cggccaccac cgcgccccga ttggcccgca gccactcgcg caggccgacg
      241 tcgcctgccg gccgccgccg gtaggccagc cacgacgccg cgcccacggc cgccagcgcc
      301 aacaggatgc ctccgccgtt gttgtcggcc agccccaggc tgccggccag ccaaaaaacg
      361 tagctgacca gcagcagccc ggccatcttg acgaaggcgt agccccggtc ggccagcggg
      421 cgcagcaacg tgaagaccag cggccaggcc accagtccca gcagcgtcag gacggcccac
      481 cagcgcacgg cggaaaggat gtcggtcagg ggcatgctca gttctcaccc actattcgat
      541 caatgctcag ttgcccagcg gccgggtgtt gctcattggc cgcttgcgga gccgggctgg
      601 ccgattgcgc gtcccgctcc tggagggcga cgacgcggta gatgcgcgcg aactcgtcgc
      661 gatacacctc cgccagcgta ccgtcggcca ccatggcctc gaacttgctc agcccttccg
      721 gccagtagta ggtattctcc agcgaaccga cgacgatgta ttcgacgcca tagcgggcca
      781 ggatgttcca cgcctcgttg gtgtcgagcg tattgtagaa agtattcaca tcgttgatcc
      841 ggctggacac cagtgtatcc ggcgcgaccg cccgctgctg ccgctggtgc cagtcccagc
      901 cgacgaccgt cggcagcccg gtgtacatgg cgatacgcga ggcgatggag cgataggggt
      961 tgctgccgtg ggcttccatg accacgggcg aaccctggac gttgcgttgc agccagtcga
     1021 tggcccccat gtcctcgacc agccgcaccg tgcggctctg gccagcataa tcggtgtcgc
     1081 catactccac ggtgggcatg aaggccgcgc cgtcgagcgt ggtcggcgcg tcggggttca
     1141 tgcggatgcc ccacttagcg gccgtggcca gcagcggata gagcaaggcc gcgaagagca
     1201 tcacccccag caccgtctgc cacaccagcc gcagccgccg cgtctgacgg atggatggcc
     1261 acgcccacac cgccgccggc ccgcaggcga cgctgagcat cagccacact tgcagataga
     1321 acttgaagac ggtgttcatc cggccgatat cgccatcgag gacgacgatt tcgacgatca
     1381 gcgttagccc tagcgccgcg gccatcagcg ccagcaccac gcgccgcgcc gccggcaacc
     1441 ccggccgcag cgtcagtagc ccgctggccg ccagcagcgg caagaccacc ggggcgatcc
     1501 agtagccgcg aataagcagg gccgcgcaca ggacgacaaa cagccccgcc gccagcagca
     1561 gcggccggcc cagcggctcc aggcgtcgtt tgccctcttc ggtccacgtt gccgtccagg
     1621 cccgcaactc tcgcgccaga tgggtgacga cgaagagcag gaacagaccg tggacgagca
     1681 gaaagttcca cacgtgg
//
LOCUS       DAOSMQ010000605         1690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_71651_length_1690_cov_5.017737, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000605 DAOSMQ010000000
VERSION     DAOSMQ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71651_length_1690_cov_5.017737"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PK829_14650"
     CDS             <1..658
                     /locus_tag="PK829_14650"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121923.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOU42504.1"
                     /translation="AASAAITLAAWRERPLAERRAQLEAVANTAASPWLPLSAVIAAP
                     SWVDLASLREESRARGVEGFMLKRRASPYRVGRRRGDWWKWKIDPYSIDAVLIYAQRG
                     SGRRASLYTDYTFALWEGDDDHRQLVPFAKAYSGLTDAEMREVDRFVRNNTEDRFGPV
                     RSVRPELVMELAFESIQASNRHKSGVAVRFPRILRWRHDKRPEDADTMATLRAMIPDL
                     "
     gene            671..>1690
                     /locus_tag="PK829_14655"
     CDS             671..>1690
                     /locus_tag="PK829_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU42505.1"
                     /translation="MNCPHCHAVNREGAAFCRACGSLLLVACPTCGAAAAAGANFCDA
                     CGRPLGPAGWLGGLGGDGTRARPGLAAETTARLAPLTEAPSAGSAVVHSSAAPPALTD
                     TLQQFIPRELQDKLLAARRSGTMAGERRVVTMLFCDIKGSTALAEQLDPEEWSDIVNG
                     SFERMVRPIYHYEGTVARLMGDGLLAFFGAPIAHEDDPRRAVLAGLEIVAGMAEFRAG
                     LPRVAQALDVRVGINTGLVVVGAVGSDLRLEYSALGDAINVAARMEQTAQPGTVQITE
                     DTLRSIAGQFDVEPLGSIEVKGKAAPVPAYRVLRRRTGMELRRFQMTFRAPLVNRLRE
                     WDLLRG"
BASE COUNT          248 a          618 c          569 g          255 t
ORIGIN      
        1 cgcggcatcc gctgcgatca cgctggccgc gtggcgcgag cgcccgctgg cggagcgccg
       61 ggcacagttg gaggcggtgg ccaacacggc cgccagcccc tggctgccgc tgtcggccgt
      121 catcgccgcg ccgtcgtggg tcgatctcgc atcgctacgc gaagaaagcc gcgcccgtgg
      181 cgtcgagggc ttcatgctca agcgccgcgc ctccccctac cgcgtcggcc gccgccgcgg
      241 cgactggtgg aagtggaaga tcgaccccta cagcatcgac gccgtgctca tctatgccca
      301 gcgcggcagc ggccgccgcg ccagcctcta caccgactat accttcgccc tctgggaggg
      361 cgacgacgac caccgccagc tcgtgccctt cgccaaggcc tacagcggcc tgaccgacgc
      421 cgagatgcgc gaggtcgacc gttttgtgcg caacaacact gaggatcgct tcggccctgt
      481 gcgcagcgtg cggccggagc tggtcatgga actggctttc gagagcattc aggccagcaa
      541 ccggcacaaa tcgggcgtgg ccgtgcgctt cccgcgcatc ctgcgctggc ggcacgacaa
      601 gcggccggaa gacgccgaca caatggccac tttacgagcg atgatccccg atctataatg
      661 tcggccagag atgaactgcc cacactgcca cgcggtcaat cgggagggag ccgcgttctg
      721 tcgcgcctgc ggcagtctgt tgttggtcgc ctgccccacc tgtggcgcgg cggccgcggc
      781 cggagccaac ttctgtgacg cttgcggccg ccccctgggg ccggcggggt ggctgggtgg
      841 cctgggtggc gatggaaccc gcgcccggcc agggctggcc gccgaaacaa cggcccgtct
      901 tgcccctcta accgaagctc cgtccgccgg tagcgcggtc gtccacagca gcgccgcccc
      961 gcccgccctc acggacaccc tgcaacagtt cattccccgc gagttgcagg acaagctgct
     1021 ggccgcccgg cgctccggca cgatggccgg cgagcgccgc gtcgtcacca tgctcttctg
     1081 cgacatcaaa ggctctaccg ccctggccga gcaactcgac ccggaggagt ggagcgacat
     1141 cgtcaacggc agcttcgagc gcatggtgcg gcccatctat cactatgagg gcacagtcgc
     1201 ccggctgatg ggtgacgggc tgctggcctt ctttggcgcg cccatcgctc acgaggacga
     1261 tccccgccgc gccgtcctgg ccgggctgga gatcgtcgcc ggcatggccg agtttcgcgc
     1321 cgggctgccg cgcgtggccc aggccctcga cgtgcgcgtt ggcatcaaca ccggtctggt
     1381 cgtcgtcggc gcggtcggct ccgacttgcg gctggaatac agcgccctgg gcgacgccat
     1441 caacgtcgcc gcgcgcatgg aacagacggc ccagccgggc acggtgcaga tcaccgagga
     1501 caccctgcgc tccatcgccg gccagttcga tgtcgagccg ttgggcagca tcgaggtgaa
     1561 gggcaaagcc gcgcccgtgc cggcctaccg cgtcctgcgg cggcgcacgg gcatggagtt
     1621 gcgccgcttc cagatgacct ttcgcgcccc cctggtgaac cgcctgcgcg agtgggacct
     1681 gctgcgcggc
//
LOCUS       DAOSMQ010000606         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_71800_length_1688_cov_3.689528, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000606 DAOSMQ010000000
VERSION     DAOSMQ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71800_length_1688_cov_3.689528"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PK829_14660"
     CDS             complement(<1..1223)
                     /locus_tag="PK829_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOU42506.1"
                     /translation="MGRVTDQPSRLMLLTTVRSYRMEAFRAAADRLGIAVVTGLDLPP
                     ELADQWPEALPLSFAAPDEAAAGIAAYAAAHPLAAILSLDDSGSLVAAAASRALGLPH
                     NDPAAAVAARDKFVMRRLLREGGVATPWFERFTTADDPAVVAARAPYPCVVKPLNLNG
                     SRGVIRADSPAELIAAVGRLTRLIAPYGPPRPYLIEAFIPGFEVALEGLLDDGQLRVL
                     ALFDKPDPLDGPFFEETIYVTPSRLPGATQTAIAATTAAAARALGLCAGPVHAELRVN
                     ADGAWLVEVAGRSIGGLCSQVLRFGIDASLEELILRQAVGLPLGDTGRRDRAAGVMMI
                     PIPQAGLLRAVEGVEEAAAVPLIDGVEITARLHYPLTPLPEGDSYLGFIFARGETPGE
                     VEAALRQAHGRLRFA"
     gene            1334..>1688
                     /locus_tag="PK829_14665"
     CDS             1334..>1688
                     /locus_tag="PK829_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42507.1"
                     /translation="MRNTTPAHPQPIDRPRALSGRQLVALATVTFAAVLLSLAAACRG
                     LGVESASSISEFQVVSEAAISAPSGYIPAAFGSPLDPTATPTPSPSPTPEIGPPLPAP
                     TVVVAVPPIDFEAARQ"
BASE COUNT          260 a          600 c          602 g          226 t
ORIGIN      
        1 atggcgaagc gcaggcggcc atgcgcctgg cgcagtgccg cctctacttc gccaggcgtt
       61 tccccacggg cgaagatgaa gcccagatag ctgtcgccct ccggcagcgg cgtcagcggg
      121 tagtgcagcc gggcggtgat ctccacgccg tcaataagag gcacggcggc ggcctcttcg
      181 accccctcca cggcccgcag tagcccggcc tggggaatgg ggatcatcat cacgccggcg
      241 gcgcgatcgc gtcgcccggt gtcgcccagc ggcaggccca ccgcctggcg cagaatcagt
      301 tcttccaacg aggcgtcgat gccgaagcgc agcacctgcg agcacagccc gccgatggag
      361 cgcccggcca cctcgaccag ccacgccccg tccgcgttga cgcgcagttc ggcgtggaca
      421 ggaccggcgc acaggcccag cgcgcgcgcg gccgcggctg tcgtcgccgc gatggccgtc
      481 tgcgtcgccc ccggcagccg cgacggggtg acatagatcg tctcctcgaa gaacggcccg
      541 tcaagcgggt cgggcttgtc gaacagggcc aggacgcgca attgaccgtc atccaacaac
      601 ccctccaggg ctacctcgaa gccggggatg aaggcctcga tgaggtaagg gcgcggcggg
      661 ccgtaggggg caatgagccg cgtcaaccgg ccgaccgccg cgatcaactc cgccggcgaa
      721 tcggcgcgga tgacgccgcg gctgccgttg aggttcagcg gcttgacgac gcaggggtag
      781 ggcgcgcgcg cggccacgac ggcggggtcg tcggccgtcg tgaagcgctc aaaccagggc
      841 gtggccacgc cgccctcgcg cagcagccgg cgcatcacga acttgtcgcg ggcggcgacg
      901 gcggcggcgg ggtcgttgtg gggcaggccc agcgcccggc tggcggcggc ggcgacgagg
      961 ctgccgctgt cgtccaagga gaggatggcc gccaggggat gcgcggcggc ataggcggcg
     1021 atgccggccg cggcctcgtc aggcgcggcg aaggacagcg gcagcgcctc cggccactgg
     1081 tcggccaact ctggtggcaa gtcaaggccg gtgaccaccg cgatgcccag ccggtcggcc
     1141 gcggcgcgga acgcctccat tcggtaggaa cgcacggtcg tcagcagcat gaggcgtgat
     1201 ggctggtcgg tcacgcggcc cattatagca ccgcgcgact gacagcactt ttttacgttc
     1261 tcctcactga ccgggcggtc aattgcggct atagtagggt tggagaagcc ctctgcggca
     1321 aggagaagga gcattgagaa acacgacgcc tgcccacccg cagcccattg accgcccgcg
     1381 cgccctctca ggccggcagc ttgtggccct ggccacggtg acctttgcgg ccgtgctgct
     1441 gtcgctcgcc gccgcctgcc gggggctggg cgtcgaatca gcctcgtcga ttagcgagtt
     1501 tcaggtcgtc agcgaggccg ccatatcggc cccatcgggc tacattccgg cggcgttcgg
     1561 cagcccactc gacccgacgg ccacgccaac gccatcgccc tcgccaacgc cggagatcgg
     1621 cccgccgctg cccgcgccga cggtcgtcgt cgccgtgccg ccgatcgact ttgaggccgc
     1681 gcgccagg
//
LOCUS       DAOSMQ010000607         1676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_72493_length_1676_cov_4.567551, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000607 DAOSMQ010000000
VERSION     DAOSMQ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72493_length_1676_cov_4.567551"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PK829_14670"
     CDS             <1..624
                     /locus_tag="PK829_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOU42508.1"
                     /translation="IVERTNPRVIRVVALGIPSDVEKTQWYFQRWVAQLPAAGEIVLF
                     DRSWYTRAITEQVHGFCTEQEYREFLSSCPQFERMLQRSGIILLKYWFSVSDDEQERR
                     FAQRAADPRRRWKLSPMDLRSRDMWVEYSQAKDRMFAYTDTKQSPWYVVNADDKRRAR
                     LNCISHILSVIPYEDILPPPIELPPRKAAGGYIRPPIDEQTFVPERY"
     gene            628..>1676
                     /locus_tag="PK829_14675"
     CDS             628..>1676
                     /locus_tag="PK829_14675"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOU42509.1"
                     /translation="MAARRTRLLWLGLLLLALALGGIYVYHTFLSRVNFAGSVGAGGA
                     ATVRVPPGFSVNVFAEGLDGPRFIAFGPDGVLYVAERGAGRIVALPDGDGDGRADALQ
                     PVAEGLDRPHSLVYHEGAWYVGLPGSVVRLAPDGGSGTTPTVVVGDLPSGGAHTTRTV
                     AFLPDGRMVVAIGSSCNVCVEDDPRRAAIMVYNADGSDGRLLATGLRNAVGLAIEPES
                     GALWATNNGRDLMGDDTPPETVVIVNDGDHFGWPACHAGNIIDPDFGAPGACEGVVAP
                     VVTLQAHMAPLGLAFYTGQTFPDDYRGDLFIALHGSWNRPEPVGYKVMRVPMAGGQVA
                     GPAEDFATGWLTDGQV"
BASE COUNT          239 a          587 c          578 g          272 t
ORIGIN      
        1 atcgtcgagc gcaccaaccc gcgcgtcatc cgcgttgtcg ccctgggcat ccccagcgac
       61 gtggagaaga cccagtggta ctttcaacgt tgggtggcgc agctgccggc cgcgggcgag
      121 atcgtcttgt tcgaccgcag ttggtatacg cgcgccatca ccgagcaagt ccacggcttc
      181 tgcaccgagc aggagtaccg cgagttcctg tcgtcctgcc cccagtttga gcggatgctc
      241 cagcgctccg gcatcatcct gctgaagtac tggttctccg tcagcgacga cgagcaggag
      301 cggcgctttg cccagcgcgc ggccgacccg cgccgccgct ggaagctcag ccccatggac
      361 ctgcgctcgc gcgatatgtg ggtggagtac tcgcaggcca aggaccgcat gtttgcctac
      421 acagacacaa agcagtcgcc ctggtacgtg gtcaacgccg acgacaagcg gcgggcgcgc
      481 ctgaactgta tcagtcacat cctgagcgtg attccctacg aggacatcct gccgccgcca
      541 atcgagttgc cgccgcgcaa ggccgccggc ggctacatcc ggccgcccat cgacgagcag
      601 accttcgtgc cggagcgcta ctaacccatg gcggcgcggc ggacgcgcct gctgtggctg
      661 gggttgctgc tgctggccct ggcgctgggc ggcatctatg tctaccacac ctttctgagc
      721 cgggtgaact tcgccggttc tgtcggcgcg ggcggcgcgg ccaccgtgcg cgtgccgccg
      781 ggcttcagcg tcaacgtctt tgccgagggg ctggacggcc cgcgcttcat cgccttcggg
      841 ccggacggcg tgctctacgt ggccgagcgc ggggcagggc gcattgttgc cctgcccgac
      901 ggcgacggcg acggccgcgc cgacgccctc cagcccgtcg ccgagggcct cgaccggccc
      961 catagcctgg tctatcacga gggcgcctgg tacgtcggcc tgcccggtag cgtggtgcgc
     1021 ctggcccctg atggcggctc cggcacgact ccgacggtgg ttgttggcga cctgcccagc
     1081 ggtggcgcgc acacaacgcg gacggtcgcc tttctgcccg acggccggat ggtcgtcgcc
     1141 atcggctcca gctgcaacgt ctgcgttgag gacgacccgc gccgggcggc catcatggtc
     1201 tacaacgccg atgggagcga tggccggctc ttggccactg gcctgcgcaa cgccgtcggg
     1261 ctggcgattg agccggagag cggcgcgctg tgggccacca acaacggccg cgacctgatg
     1321 ggcgacgaca cgccgccgga gacggtcgtg attgtcaacg acggcgacca cttcggctgg
     1381 cccgcctgcc acgccggcaa catcatcgac ccggatttcg gcgcgccggg cgcgtgcgag
     1441 ggcgtcgtcg ccccggtcgt cacgctgcaa gcccacatgg ccccgctggg gctggccttc
     1501 tacactggcc agaccttccc ggacgattat cgcggcgacc tgttcatcgc cctgcacggc
     1561 tcatggaacc ggccggagcc ggtcggctac aaggtgatgc gcgtgccgat ggcgggcggg
     1621 caggtggccg ggccggccga ggactttgcc acgggctggc tgactgatgg gcaggt
//
LOCUS       DAOSMQ010000608         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_72996_length_1667_cov_4.883995, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000608 DAOSMQ010000000
VERSION     DAOSMQ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72996_length_1667_cov_4.883995"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK829_14680"
     CDS             complement(<1..278)
                     /locus_tag="PK829_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42510.1"
                     /translation="MAATSAGPINTRPGRPAAWEVAAVLALLLLAGVARMARPDLTEF
                     KADEGRLLSLALATSDGDIARRGIASSVGFPNAPMSVWLYALPLLLRP"
     gene            complement(265..1056)
                     /locus_tag="PK829_14685"
     CDS             complement(265..1056)
                     /locus_tag="PK829_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOU42511.1"
                     /translation="MAMLKLISWNVNGLRAVHRKGLFLEWLAATQPDVLALQETKCHP
                     DQLPPEVRQPPGYMTYWASAERPGYSGVALYTRRAPLSVQIGLGSDDYDREGRTLVAD
                     FGDFVLINAYFPNGGRDHARVPFKMRYKADFLTTCNNLRAAGRAVVFCGDVNTSHQDI
                     DLARPRQNRNTTGFLPEERAWIDEVVAQGYVDTFRARYPDRAGAYTWWAQVTFSREKN
                     VGWRLDYFFVSPDLWPQVIDATIEADVSGSDHCPVGLLLDLDGRD"
     gene            1114..>1667
                     /locus_tag="PK829_14690"
     CDS             1114..>1667
                     /locus_tag="PK829_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU42512.1"
                     /translation="MDARTDRPTALYDLSRAALTERLAGWGYSDYYAGLVWAGLYRDL
                     AAAPEAIAGLRPELAARLAAETIGVLPVRATIDSADGHTRKLLLGLADDAAIETVLMT
                     FRGRATVCVSTQVGCAMGCVFCATGQMGFHRHLSPGEIVGQVIHAERTLRARGETLRN
                     IVLMGMGEPLHNYEATMAAIETVM"
BASE COUNT          269 a          556 c          593 g          249 t
ORIGIN      
        1 tgcggcctta gcagcagcgg caaggcatag agccagacgc tcatgggcgc gttggggaag
       61 ccaacggagc tggcgatgcc gcgccgggcg atgtcgccgt cgctggtggc cagggccagg
      121 ctcagcaggc ggccctcgtc ggccttgaac tcggtcaggt cgggccgcgc catccgcgcg
      181 acgccggcca acaatagcag ggccaggacg gcggccactt cccacgccgc cggccgcccc
      241 ggtcgggtat tgatcggccc ggcgctagtc gcggccatcg aggtccagca acaaacccac
      301 cgggcagtgg tcggagccgc tcacgtcggc ctcgatggtg gcgtcgatca cctgcggcca
      361 gaggtcgggc gagacgaaaa agtagtccag ccgccagccg acattcttct cgcgcgagaa
      421 agtgacctgc gcccaccagg tgtaagcccc ggcgcggtcg ggatagcggg cgcggaaggt
      481 atcgacgtac ccctgggcaa cgacctcgtc gatccaggcg cgctcttcgg gcaggaagcc
      541 ggtggtgttg cggttctggc gcggccgggc caggtcgatg tcctgatggg aggtgttgac
      601 gtcgccgcag aaaacgaccg cgcgccccgc ggcccgcagg ttgttgcaag tggtcaggaa
      661 gtcggcctta tagcgcatct tgaagggcac gcgcgcgtgg tcgcggccac cgttggggaa
      721 gtaggcgttg atgagcacga aatcgccgaa gtcggctacc agcgtccgcc cctcgcggtc
      781 gtagtcgtcg ctgcccagcc cgatctggac cgacagcggc gcgcggcggg tgtagagggc
      841 cacaccgctg taaccagggc gctcggccga ggcccaatag gtcatgtagc ccggcggctg
      901 gcgcacctcc ggcggcaact ggtcgggatg gcacttcgtc tcctgcaagg ccaggacgtc
      961 cggctgggtc gccgccagcc actccagaaa cagccccttg cgatggacgg cccgcaggcc
     1021 gttgacgttc cagctaatca gcttgagcat ggccacattg tagctggctt agccccgcct
     1081 gacagcttcg ggcaacctgt tacaatggcg ggcatggacg ccagaacgga tcgccccacc
     1141 gcgctctacg acctcagtcg cgccgccctg accgagcggc tggccggttg gggctatagc
     1201 gattattacg ccgggctggt gtgggcgggc ctctaccgcg acctggccgc cgcgccggag
     1261 gccatcgccg ggctgcggcc ggagctggcc gcgcggttgg cggcggagac aatcggcgtc
     1321 ctgcctgtcc gggcaaccat cgacagcgcc gacggccaca cccgcaagct tctgctgggc
     1381 ctggccgacg acgcggccat tgagacggtg ctgatgactt tccgcggccg ggcgaccgtc
     1441 tgcgtcagca cacaggtcgg ctgcgccatg ggctgcgttt tctgcgccac ggggcagatg
     1501 ggcttccacc gccacctgtc gccgggcgag atcgtcggtc aggtcatcca cgccgagcgg
     1561 acgctgcgcg cccgcggcga gacgctgcgc aacatcgtcc tgatgggcat gggcgagccg
     1621 ctgcacaact atgaggccac gatggcggcg atagaaacgg tgatgga
//
LOCUS       DAOSMQ010000609         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_73022_length_1667_cov_2.725806, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000609 DAOSMQ010000000
VERSION     DAOSMQ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73022_length_1667_cov_2.725806"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1667)
                     /locus_tag="PK829_14695"
     CDS             complement(<1..>1667)
                     /locus_tag="PK829_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42513.1"
                     /translation="LAAVQLVPFLQFSLASERLATADYAFATDYSLPPAHLITLLVPE
                     FFGEPLRVGYWSVPTFEELTYYAGGLAVLGLVLALRRPTRLTWFYVALIVGGLWLALG
                     RYSILYELAYRFLPPFRLVRAPGRAGFLYLFAASALLAHTLTFWRTMPAAERRRRLWP
                     FWPAAVAVLAVALVAALAATGAVFMAVHPTETSGRLWQQIGGYALALTVVGIGGALVW
                     AYLMRPEVPAAPAVGVVERDAVGERDAVGERDAGGGRDAGRWRDAGRWRGAGRVALPA
                     ALVVLVMADTWSYAHKMVRLADSGPDPVWTDGLAVVGAAGGEEPAGRVLPWGVPLFSQ
                     NGAVQVGWPSVFGYDSLEPAATITLAASVPDPRSTAYDILGAAYVLAGGPLDDFTAGE
                     RPLTLLGQQGSAWVYRRARPLPLSRLVYAAEVIADSRAAIARVHEADFDPTTTAIQAA
                     DPGCALGPAPATMGTAEVLAHEATGWQIQTRSDAPALLVLAENAFPGWQVTIDGRPAA
                     ALTAYTAIRAVCVPAGEHVVEWRFRPVIYLFGAALSVVALVAVVVAW"
BASE COUNT          315 a          643 c          549 g          160 t
ORIGIN      
        1 ccacgcaaca acgacggcaa ccagcgccac cacgctcaga gccgcgccga agaggtaaat
       61 caccggccgg aagcgccact ccacgacatg ctcccccgcg ggcacacaga ccgcgcggat
      121 ggccgtataa gccgtcagag ccgcggccgg gcggccgtcg atggtcacct gccagccggg
      181 gaaagcgttc tcggccagca cgagcagcgc cggcgcgtcg ctgcgggtct gaatttgcca
      241 gcccgtggcc tcatgggcca gcacctcagc cgtgcccatc gtcgccgggg ccgggccgag
      301 tgcgcagccg gggtcagcgg cctgtatagc cgtcgtcgtc gggtcgaagt cagcctcatg
      361 gacacgggca atggccgcgc ggctgtcggc aatgacctca gcggcataga ccagccgcga
      421 cagaggcagc ggtcgcgccc ggcgatagac ccaggccgac ccctgctgcc ccagcagcgt
      481 caacggccgc tcccccgcgg tgaaatcgtc cagcggcccg ccggccagca catacgccgc
      541 gccgagaatg tcataggccg tcgagcgcgg gtcgggcaca gacgcggcca gggtaatcgt
      601 cgccgccggt tccagcgagt catagccaaa gacactgggc cagccgacct gcaccgcccc
      661 gttctggctg aacagcggca cgccccaggg caacacccgc cccgctggct cctcgccgcc
      721 ggccgcccca accacggcca acccgtccgt ccagaccggg tccggcccgc tgtcggccag
      781 gcgaaccatc ttgtgagcgt agctccacgt gtcggccatg accagcacaa ccagagccgc
      841 aggcagggcc acccggccag cgccgcgcca gcggccagcg tcgcgccagc ggccagcgtc
      901 gcgcccaccg ccagcgtcgc gctcgccgac agcgtcgcgc tcgccgacag cgtcgcgctc
      961 gacgacgccc actgccgggg ccgcgggcac ctccggccgc atcaggtagg cccagaccag
     1021 cgcgccgccg atgccgacaa cggtcagggc cagcgcatag ccgccgatct gctgccacag
     1081 gcggccgctg gtctccgtgg gatggacggc catgaagacc gcgcccgtgg ccgccagcgc
     1141 cgccaccaga gccacagcca gcaccgccac agccgccggc cagaagggcc acagccgccg
     1201 ccgtcgctcg gccgcgggca tcgtccgcca gaaagtcagc gtatgggcca gcagcgccga
     1261 ggcggcgaac agatagagaa agcccgcccg ccccggcgcg cgcaccagcc ggaacggcgg
     1321 caagaagcga taggccaact cgtagaggat gctatagcgg cccagagcca gccacagccc
     1381 gccgacgatg agagcgacgt agaaccaggt cagccgcgtc ggccgccgca gggccaacac
     1441 cagccccagc accgccagcc cgcccgcgta ataggtcaac tcctcgaagg tcggcacgct
     1501 ccagtagccg acgcgcagcg gctcgccaaa gaactccggc accagcaaag tgatgagatg
     1561 ggccggcggc agcgagtagt cggtagcgaa ggcataatcg gccgtcgcca gccgctccga
     1621 ggccagactg aactgcaaga agggcacaag ctggacagcg gccaggg
//
LOCUS       DAOSMQ010000610         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_73069_length_1666_cov_3.959032, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000610 DAOSMQ010000000
VERSION     DAOSMQ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73069_length_1666_cov_3.959032"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PK829_14700"
     CDS             <1..645
                     /locus_tag="PK829_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU42514.1"
                     /translation="WFHSRYDRRPDFVAARVDEPPKKSGLLLLRRIAAEFGVRTRRAT
                     EDQLNELRDFLTETTARSVLPLVIIDEAHELEPEQYKELRRLLNFRDPRGGKAYQLIL
                     LGRPELDINLRGSKDFNDRVATRSSLDPLAPDDTAALIEYRLLAAGRENRMPPLFLPD
                     SILPIWRETRGYPRSICLLCLHLCLELLVTGGATIDGAFVARFLESHSGYRQAE"
     gene            711..1289
                     /locus_tag="PK829_14705"
     CDS             711..1289
                     /locus_tag="PK829_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42515.1"
                     /translation="MKSNERPKARDKADEQKVSTLKELLRDKPRRGRPRRMVARQNVY
                     VAMRPEQKGLMDALGERLPLGLGRPDVPDVAVSVLTARLEALRSAVAGRTREIPEGVT
                     DLESLFLLWDLTPPQPEAAGAPERWTSIRLSPQQALELGRATGTLNAAFGAGRSEVFG
                     LALALLAQFLERYPLAGAEMDLGEVREMIFRV"
BASE COUNT          273 a          530 c          601 g          262 t
ORIGIN      
        1 tggttccaca gccgctatga ccgccggccg gacttcgtgg ccgcgcgggt ggatgagccg
       61 cccaagaaga gcggcctgct gctgctgcgc cgtatcgccg ccgagtttgg cgtgcgcacc
      121 cgccgcgcca ccgaagacca gctcaacgag ctgcgcgact tcctgactga gacgaccgcc
      181 cgcagcgtcc tgccgctggt catcatcgac gaggcccacg agttggagcc ggagcagtat
      241 aaggaattgc ggcggctgct caatttccgc gacccgcgcg gcggcaaggc ctaccagctc
      301 atcctcctcg gccggcccga actggacatc aacctgcgcg gcagcaagga cttcaacgac
      361 cgcgtggcca cccggtcgtc gctcgacccg ctggcccccg acgacacggc ggcgctgatc
      421 gagtatcgcc tgctggccgc cggccgcgaa aaccgcatgc cgcccctctt cctgcccgac
      481 agcatcctgc ccatctggcg cgagacgcgc ggctatccgc gctccatctg cctgctgtgc
      541 ctgcacctgt gcctggaact gctggtgacg ggcggggcga ccattgatgg cgcgtttgtc
      601 gctcgctttc tggagagcca cagcggctac cggcaggcgg aatagaatct ggaatcagga
      661 atgaagaggc gcggcgttgt gccacttctt ttcctaattc gtaattgatt atgaagagca
      721 acgagcgtcc caaggccagg gacaaggccg acgagcagaa ggtcagcacg ctgaaggagt
      781 tgctgcgcga caagcccagg cgcggccggc cgcggcgcat ggtggcgcgg caaaacgtct
      841 acgtggccat gcggccggag cagaaagggc tgatggacgc gctgggcgag cggctgccgc
      901 tggggctggg gcggccggac gtgcccgacg tggccgtgtc ggtgctgacg gcgcggctgg
      961 aggcgctgcg ctcggccgtc gccgggcgca cgcgcgagat ccccgagggc gtcactgacc
     1021 tggagtcgct ctttctcctg tgggacctga cgccgcccca gccggaagcg gccggcgcgc
     1081 cggagcgctg gacgagcatc cgtctatcgc cgcagcaggc gttggaactc ggccgcgcca
     1141 ccgggacgct gaacgcggcc ttcggcgcgg ggcgcagcga ggtctttggt ctggccctgg
     1201 cgctgctggc ccaattcctg gagcgctatc cgctggccgg ggcggagatg gatttgggcg
     1261 aggtgcggga gatgatcttc cgggtgtgag cggcgcgggc gaagaggcga ccgggcggcc
     1321 ggggggcggc cgccggggcc agccagcgcc ctctgcagcg aaaatccctt caaaaagacc
     1381 ccatttgctt attagtggct cataacaaat aaccctaccg ccgccatttc cttcgcatag
     1441 tgttggaagg ggagcggcgc gttttggccg gctgccgggg gagcggttgg gccgaaagtg
     1501 ggtggcggac tgcacctgcg acgccgattg agcgcgcttg tgggaggaaa tcggcatacc
     1561 aggccgccta acggtggcta ggtgggaact ggctggagac agagaaggat cgaatccggg
     1621 ttcaggatga ggttcggccg cggggcgggc cctaagggtg tgccga
//
LOCUS       DAOSMQ010000611         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_73272_length_1663_cov_2.997512, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000611 DAOSMQ010000000
VERSION     DAOSMQ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73272_length_1663_cov_2.997512"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1170
                     /locus_tag="PK829_14710"
     CDS             361..1170
                     /locus_tag="PK829_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOU42516.1"
                     /translation="MLRPMLPGPPNHLFDGYIFDLDGTVTLGEALLPTAGATIAHLRA
                     LGRRTLFLSNYPNRTRAELAARLTGMGLPTPAADVINSSWVLVQFLRARLPGARLFVV
                     GEVSLRGELAAAGFAVADDARVDAVIASFDRTFTYRKLQIAFDAIRGGARFFATNGDR
                     YCPVPGGGQPDAAAIIAAIEACTNTRVEAVVGKPSAHMAAAALAALGLPPERCLMTGD
                     RLETDVQMGLDAGLATALALTGATDAAMLAASPVRSDFALRQLSDLLPDGS"
     gene            complement(1276..>1663)
                     /locus_tag="PK829_14715"
     CDS             complement(1276..>1663)
                     /locus_tag="PK829_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42517.1"
                     /translation="LATPAARPQPPRGWTVEKLLGEIQCNLYVGLQRFRRGERQAAAR
                     EVQLYAVDRLLQLAAVVEPEGAAAADSFAPARRFEQRFPQMAAHLPTFQQGYDRTPES
                     AAAILDFLAARFELNAGMVAAIRELL"
BASE COUNT          264 a          559 c          576 g          264 t
ORIGIN      
        1 atgacgtaga gcgccgccgg gttggtctgg gcatagaagg ccaggccgac ggccaggctg
       61 aagagcgcga tcaggagcca cgccgggctg agaaggatga caagtaagcg acgcatggta
      121 tcgatggtgg tcaaggatgt tgacggctat tgtagtccag gcaatggcca ttgccaatcg
      181 gtaaagaatg gcacgcagga caaccgcata tagtatatta gcaccggaat ggcacgccga
      241 tgacgctgac tgggcgctga tttacgctgc aagacttgct ctttcagcgt cgatctgcgt
      301 gcaatcagcg tcatcagcgt gccattcttc tctccctgcc caaaccggcc cggttgggtt
      361 atgctacgac ccatgctgcc cggcccgccc aaccacctct tcgacggcta catcttcgac
      421 ctcgacggca cggtgacgct gggcgaggcg ctgctgccca cggccggcgc gaccatcgcc
      481 cacctgcgcg ccctcggccg gcggacgctc ttcctgtcca actaccccaa ccgcacgcgg
      541 gcggaactgg ccgcgcggct gacgggcatg gggctgccca cgccggcggc cgacgtcatc
      601 aactcgtcgt gggtgctggt gcagttcctg cgggcgcggc tgccgggggc gcggctgttc
      661 gtcgtgggcg aggtgtcgct gcggggcgaa ctggccgcgg ccggctttgc cgtggccgac
      721 gacgctcgcg tcgatgccgt catcgccagc ttcgaccgga cgttcaccta tcgcaagctg
      781 caaatcgcct tcgacgccat tcgcggcggg gcgcgcttct ttgccaccaa cggcgaccgc
      841 tattgccccg tgcccggcgg cggccagccc gacgccgcgg ccatcatcgc cgccatcgag
      901 gcctgcacca acacgcgcgt ggaggccgtc gttggcaagc cctcggccca catggccgcg
      961 gcggcgctgg cggcgctggg cctgccgccg gagcgctgcc tgatgaccgg cgaccggctg
     1021 gagacggacg tgcagatggg gctggacgcg ggcctggcca cggccctggc cctgaccggg
     1081 gcgacggacg cggccatgct ggcggcctcg cccgtgcgct cggacttcgc cctgcggcag
     1141 ctaagcgacc tgctgccgga tggctcgtag cgcccgcccc aggcgaagaa cctcacgcaa
     1201 aggacgccaa gggagcaaag ggcgcaaaaa gaaggcatct tgatatcctt tgtgtgaggt
     1261 tttcctgtca acggtttaga gcaactctcg tatcgccgcg accatgccgg cgttcaactc
     1321 gaagcgtgcc gccaggaagt cgaggatggc cgcggccgac tccggcgtgc ggtcgtagcc
     1381 ctgctggaag gtgggcaggt gagcggccat ctgcgggaag cgctgctcga agcggcgcgc
     1441 cggggcgaag gagtcggccg cggcggcccc ttccggctcc acgactgccg ccagttgcag
     1501 cagtcggtcg acggcgtaga gctggacctc acgcgccgcg gcctgtcgtt cgccacgccg
     1561 gaagcgctgt agaccgacgt agagattgca ctggatttcg cccagcagct tctcgaccgt
     1621 ccagccgcgc ggcggctggg gtcgggccgc cggcgtggcc agg
//
LOCUS       DAOSMQ010000612         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_73382_length_1661_cov_4.310710, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000612 DAOSMQ010000000
VERSION     DAOSMQ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73382_length_1661_cov_4.310710"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK829_14720"
     CDS             complement(<1..991)
                     /locus_tag="PK829_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HOU42518.1"
                     /translation="MPHPTPFHTRTSAVCDSHEWRNWSGFLSAGVYEPSHEREYYAIR
                     NSAGLIDVSPLYKYEVTGPDAARLVDRVITRDVTKQKVGQVYYTPWCDARGKVIDDGT
                     VSRLAADRFRITSADPNGRWFQDVGYGLNATVTNVTDDLAALALQGPRSRAILEEVVV
                     GVDFSKLPYFHLAHGTVDGFPVTVTRTGYTGDLGYELWVRPEYAERLWDGLIDRGNGH
                     GILPAGIIALDVARVEAGLVMIAVDYVSSHHAIIESQKSSPYEIGLGWAVRLDKGDFI
                     GKRALVAERERGSAWTFVGVQCFWPALERLFAEEGLPPQVAGRASRSAVPVYEQ"
     gene            complement(1031..>1661)
                     /locus_tag="PK829_14725"
     CDS             complement(1031..>1661)
                     /locus_tag="PK829_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU42519.1"
                     /translation="IDRDAADTYRLGSGLIALGARAQRANPVRLRARPELEVLAAGTG
                     ETATLEVLSGGEVVILDEIPGEYVTSGSQHIGSRWPVYATSTGKAILAHLPPQELARA
                     LAGPFPPLTAHTLVDAERLRACLAEQRHAGYAVAAEELEVGFVAVGAALLDARGYPVA
                     AISLGGTRARLTDERVPAIGGLVRAAAGRISRQLGYRPDSLAEPGDAVA"
BASE COUNT          256 a          570 c          589 g          246 t
ORIGIN      
        1 cctgctcata gacgggcaca gccgagcgcg aggcccgccc ggctacctgc ggcggcaagc
       61 cttcctcggc gaacagccgc tccagcgccg gccagaagca ctgtacgccg acgaacgtcc
      121 acgctgaacc gcgctcccgc tcggccacca gggcccgctt gccgatgaag tcgcccttat
      181 ccagccgcac cgcccagccc aggccgatct cgtagggcga cgacttctgg ctctcgataa
      241 tggcatggtg gctggagacg taatcgacgg cgatcatcac cagccccgcc tccacgcggg
      301 cgacgtcaag ggcgatgatg cccgcgggca ggatgccgtg gccgttgccg cggtcgatca
      361 ggccatccca caggcgctcg gcatactccg gccgcaccca caactcatag cccaggtcgc
      421 cggtgtagcc ggtgcgcgtc acggtgaccg ggaagccatc gaccgtgccg tgggccaggt
      481 ggaagtaggg cagcttgctg aagtcgacgc ccacgacgac ctcttccaga atagcccggc
      541 tgcgcggccc ttgcagcgcc agcgcggcca gatcgtcggt gacgttggtc acggtcgcgt
      601 tcaggccata gcccacgtcc tggaaccagc ggccgttggg gtcggccgag gtgatgcgga
      661 agcggtcagc cgccagtcgc gagactgtgc cgtcgtcgat gaccttacca cgggcgtcgc
      721 accagggtgt gtagtacacc tggccgacct tctgtttggt cacgtcgcgg gtgatgacgc
      781 ggtcgaccag gcgggcggcg tcggggccgg tgacttcgta cttgtagagc ggcgaaacgt
      841 cgatcaaccc ggcgctgttg cgaatggcgt aatactcgcg ctcatgcgac ggttcataga
      901 cgccggccga caggaagcct gaccagttgc gccactcatg gctatcgcag acggccgacg
      961 ttcgtgtgtg aaagggggtg gggtgcggca ttgttgctcc ttgctcagtt gccggcgaca
     1021 ggctgacagc ctatgctacg gcgtcgcccg gctcggcgag tgaatcgggg cggtagccca
     1081 gttggcgcga gatgcgcccg gcggcggcgc ggacgaggcc gccgatcgcc ggcacgcgct
     1141 cgtcagtcag ccgggcgcgc gtaccgccca ggctgatggc ggccacgggg tagccgcggg
     1201 catcaagcag cgccgcgccg acggcgacga agccaacctc cagctcctcg gcggccacgg
     1261 catagccggc gtggcgctgt tcggccaggc aggcccgcag gcgttccgcg tcgacgagag
     1321 tgtgggccgt cagcggcggg aacggcccgg ccagggcgcg ggccaactcc tgtggtggca
     1381 ggtgggccag gatggccttg cccgtcgagg tggcgtagac cggccagcgg ctgccgatgt
     1441 gctggctgcc gctggtgacg tactcgccgg gaatctcatc cagaatgacc acctcgccgc
     1501 cagacaatac ttccagcgtc gccgtctcgc ccgtgcccgc ggccagcacc tccagttccg
     1561 gccgcgcccg caggcgcacc gggttggccc gctgcgcccg cgcgcccagg gcgatgaggc
     1621 cggagccgag gcgataggtg tcggcggcgt cgcggtcgat g
//
LOCUS       DAOSMQ010000613         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_73512_length_1659_cov_3.909601, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000613 DAOSMQ010000000
VERSION     DAOSMQ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73512_length_1659_cov_3.909601"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PK829_14730"
     CDS             <1..806
                     /locus_tag="PK829_14730"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42520.1"
                     /translation="PAEFLCARLADAGGFHHPAVYMAEAVRLGITVRPPHVNFSDHGF
                     TLTYEHAPDSEQPVLWMGLGQVRDLRAAAIAAIRAARAGAPFAGVADLLARVPLQPKE
                     IDHLIRCGALDGLGASRRAMLAEARDTRGRGGQLRFGFLDAPDSASESPAERLAWEME
                     LLGQPVSVHPLALVHVPRGATPLGRLPAAPGQPATVYAVRVPGWPGGAGIFIGDGETF
                     VIARFDKHLASDRGRRPTWRPLRLSGRWRRDEWEGGWLQVEGVEIVDSD"
     gene            868..1326
                     /locus_tag="PK829_14735"
     CDS             868..1326
                     /locus_tag="PK829_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42521.1"
                     /translation="MRRRVTMTVVLIVALAALAACALGADEGDKGAAVDEGAESLAGG
                     YTWRREGGIAGFCDVVELTPDGAAEVSSCASDPPRPVAQVRLSTAQASLIAGWVVRFG
                     SFERQETDPATADAMTISVEFDGQGDEQPDEGDVAAMIDLAQQVLLEATR"
BASE COUNT          224 a          552 c          611 g          272 t
ORIGIN      
        1 tgccggccga gttcctttgc gctcgcctgg ccgacgccgg tggcttccac cacccggccg
       61 tctacatggc cgaggccgtc cggctgggca tcactgtgcg tccgccccat gtcaacttca
      121 gcgaccacgg cttcaccctg acctacgagc acgcgcccga cagcgagcaa cctgtcctgt
      181 ggatggggct gggtcaggtg cgcgacctgc gcgccgccgc catcgccgcc atccgcgcgg
      241 cacgggcggg cgcgcccttc gccggcgtag ccgacctgct ggcgcgcgtg ccgctccagc
      301 ccaaggagat cgatcatctc atccgctgtg gcgcgctcga cgggttgggg gccagccgcc
      361 gggccatgct cgccgaggcc cgcgacacac gcgggcgcgg cggccaactg cgcttcggct
      421 tccttgacgc gcccgactct gcgtccgagt cgccggccga gcgtctggcc tgggagatgg
      481 agctgctggg ccagccggtg agcgtccacc cgctggcgct cgtccacgtg ccgcgcgggg
      541 cgacgcccct ggggcggctg ccggccgcgc cgggccagcc ggcgacggtt tacgccgtgc
      601 gcgtgcccgg ttggcccggt ggcgcgggca ttttcatcgg cgacggcgaa acgtttgtca
      661 tcgcccgctt cgacaagcat ctggccagcg accgtggccg gcggcccacc tggcggccgc
      721 tgcgcctgtc cggccgttgg cggcgcgacg agtgggaggg gggttggtta caggtggaag
      781 gagttgagat agtggatagc gactagcgac cggtggttag tggctggtga tgcatgcggg
      841 cgtcagcctg ctgaggagag gaatgacatg agaagacgtg tgacgatgac ggtggtgctg
      901 atcgtggctc tggcagcgct ggcggcgtgc gcgctggggg ccgatgaggg tgacaagggc
      961 gcggcggtgg acgaaggcgc ggagtcgctg gcgggcggct acacctggcg gcgcgagggc
     1021 ggcattgccg gcttttgcga tgtcgtcgaa ctgacgcccg atggcgcggc cgaggtgtcc
     1081 agttgcgcca gcgacccgcc gcggccggtg gcccaggtgc ggctttccac ggcccaggcg
     1141 tcgctcatcg ccggctgggt cgtccgcttt ggctccttcg agcgccaaga gaccgacccg
     1201 gccacggccg atgccatgac catcagcgtc gagttcgacg gccagggcga cgaacagccg
     1261 gacgagggcg acgtcgccgc gatgatcgac ctggcccagc aagtcctgtt ggaggcaacc
     1321 cggtagagac gggtcgccga cccgtctctt ctcaggggag acgggtggcc gcccatcttc
     1381 tctgggagac gggtggccgc ccatcttctc tgggagacgg gtcgccgacc cgtctctacg
     1441 gcgtttgccg tcaggtttta cagattgggt caaaagcggt aacatatcga cggagcggcc
     1501 gggccgatgg gacgccggcg atgcagaccg tgtccgtccc tagatgtgcc cggcagaagc
     1561 ccttatgcgc gcgcgcccgt ttttggttac tggcattctc ggcttaggca caatcctcat
     1621 gacgctggtc atgactgcca taggcccgcg cccggccgc
//
LOCUS       DAOSMQ010000614         1658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_73573_length_1658_cov_5.313163, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000614 DAOSMQ010000000
VERSION     DAOSMQ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73573_length_1658_cov_5.313163"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PK829_14740"
     CDS             complement(<1..592)
                     /locus_tag="PK829_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42522.1"
                     /translation="MTTRILARSLRILFLFLWLPAGLLAAQPDPTPAPALATATPVAG
                     TPATDTPATSAPVTTILDSRFGVIESFEDPTAAARLGVAWTRARFQWAEVQPDGPGQW
                     QPPLDDEALQAELDAGREVIGLLIGIPDWARDRNGLPRGLSLPAGDPGNTWAVFVGQV
                     VSRYAGRIDRWIVWNEPDIDDPDAPGHFPEWLHRRRD"
     gene            685..>1658
                     /locus_tag="PK829_14745"
     CDS             685..>1658
                     /locus_tag="PK829_14745"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOU42523.1"
                     /translation="MHTYRLALIGFGNVGQGLATILRDYGPTISGQHGADFRIVAVNT
                     TRRGRIYNPDGIDPGQLLQAYSDTKRIDNVPTPYSGWDVQRVIHESNADVVIEMTTSD
                     LLTGQPATDYIRAGIAQDRHVITTNKGPVALFYPELAAMAAERGVRLGVEGTVMGGTP
                     VLRVGRELLASAGVLRIQGILNGTTNYILTRMADGEPYEAALREAQKKGYAEANPSAD
                     VDGYDAAAKIVILANLLLGIPLKMDNVEREGIGNLTTRDIVRAQQVSEVWKLVAWVER
                     TTDMVKASVRPIRLPQAHPLAGISGATNAVTFVTKLLGEVTIVGPGAGR"
BASE COUNT          297 a          561 c          552 g          248 t
ORIGIN      
        1 cgtcgcgccg cctgtgaagc cactccggaa agtggccggg cgcgtcgggg tcgtcgatgt
       61 caggctcgtt ccagacaatc cagcgatcga tgcgcccggc atagcggctg acgacctggc
      121 ccacgaagac ggcccaggtg ttgccggggt cgccggccgg cagcgacagg ccgcgcggca
      181 gcccgttgcg gtcgcgcgcc caatcgggaa tgccaataag caggccgatc acttcgcgcc
      241 cggcgtccaa ctcggcttgc agggcttcgt cgtccagcgg cggctgccac tgcccaggcc
      301 cgtcgggctg cacctcggcc cactggaagc gcgctcgcgt ccaggcaacg cccaggcggg
      361 ccgcggcggt agggtcttca aagctctcaa taacgccaaa gcggctgtcc aggatggtag
      421 tcaccggcgc gctggtcgcc ggcgtatcgg tcgccggcgt gccggccacg ggcgtggcag
      481 tcgccagcgc tggtgccggc gtggggtcgg gttgcgccgc caacaggccg gcgggcagcc
      541 agaggaacag gaacagaatg cgcaatgagc gcgcaaggat gcgggttgtc atgccagagc
      601 gtaagtgtaa ggccatacgt ggcctgacgg caagcgattg tgtgctacaa ttcccctatc
      661 tcgcagtagg acaaacatcg tcatatgcac acttaccgac ttgccctcat cggctttggc
      721 aacgtgggcc agggcctggc gaccattctg cgcgactacg ggccaaccat ctccggccag
      781 cacggggctg acttccgcat cgtcgccgtc aacaccacgc gccgcggacg catctacaac
      841 cctgatggca ttgaccccgg ccaactctta caggcctata gcgacacaaa acgcatcgac
      901 aacgtgccca cgccctactc cggctgggac gtgcaacggg tcatccatga gagcaacgcc
      961 gacgtcgtca tcgagatgac caccagcgac ctgctgaccg gccagccggc gaccgactac
     1021 atccgtgccg gcatcgctca ggatcgccac gtcatcacca ccaacaaggg gccagtggcc
     1081 ctgttctacc ccgaactggc ggcaatggcc gccgagcgcg gcgtgcgcct gggagttgag
     1141 gggacggtga tgggcggtac gccggtattg cgcgtcggcc gcgagttgtt ggcctcggcc
     1201 ggcgtcctgc gcatccaggg catcctcaac ggcacaacca actacatcct gacgcgcatg
     1261 gccgacggtg agccctatga ggcggctctg cgcgaggccc agaagaaggg ctacgccgag
     1321 gccaacccct cggccgacgt tgacggctat gacgccgcgg ccaagatcgt cattctggcc
     1381 aacttgctgc tgggcatccc gctgaagatg gacaacgtcg agcgcgaggg catcggcaac
     1441 ctgaccacgc gcgacattgt ccgcgcccag caggtcagcg aggtgtggaa gctggtggcc
     1501 tgggtggagc gcacgactga catggtcaag gcgtcggtgc ggcccatccg cctgccccag
     1561 gcgcacccgt tggccggtat ctccggggcg accaacgcgg tgacgttcgt gaccaagctg
     1621 ctgggcgaag tgaccatcgt cggccccggc gcggggcg
//
LOCUS       DAOSMQ010000615         1653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_73897_length_1653_cov_5.488110, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000615 DAOSMQ010000000
VERSION     DAOSMQ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73897_length_1653_cov_5.488110"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK829_14750"
     CDS             <1..541
                     /locus_tag="PK829_14750"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOU42524.1"
                     /translation="VFPTDTVYGIGVLPFDPVAIERLYAAKGRPAEKGIPVLLAEPAD
                     VAQIAADVPPAAAALMARFWPGPLTLIVPRQPTLPDNLSPNDTVAVRVPDHAVARALI
                     RAAGGAVAATSANVSGQPAARSAGEALAALAGRAAAVLDDGPSPGGRASTIVDCTVQP
                     PVILRVGPLSAADLGLRES"
     gene            538..1137
                     /locus_tag="PK829_14755"
     CDS             538..1137
                     /locus_tag="PK829_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU42525.1"
                     /translation="MSGLLLPLLLFVAALAATFLTYRNIRRGGARFYTLEREIILRRA
                     AFTLIIGALFYTGALGLLYYQRQQMIEAAAPPEGTPEGDTSEGAVGPGVTTPTPLFEQ
                     FPPTATATSTTPQPTITPTPVTCRAVVEGSGASGIYLRDAPQGTELVILAEGTLLTVL
                     PDEPVEAGGFLWRKVRAVGGEEGWAADDFLTIRAPCANE"
     gene            1163..>1653
                     /locus_tag="PK829_14760"
     CDS             1163..>1653
                     /locus_tag="PK829_14760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42526.1"
                     /translation="MIIGIDASRAAAARRTGTEAYAYQLIRALIPLAAERGHGLRLYF
                     NDPPPADLFPAAAHVEMRVIPLRRLWTHARLGHELRTRPPDVFFTPAHVIPIGVRVPS
                     VATVHDLGYEHFPEAHPWRQRAYLRWSTRHNARASRRVLVDSRATGDDLTRLYGTAAA
                     KIAV"
BASE COUNT          222 a          626 c          544 g          261 t
ORIGIN      
        1 ggtctttccg accgataccg tctatggcat cggcgtgtta ccctttgacc cggtggccat
       61 cgagcggctt tatgccgcca aaggccgccc ggcggagaag ggcatccccg tcttgctggc
      121 tgaaccggcc gatgtggccc agatagccgc cgatgtcccg ccggccgcgg cggcgctgat
      181 ggcccgattc tggccggggc cactgacgct cattgtgccg cgccagccga cgttgccgga
      241 caatctctcg cccaacgaca cggtagccgt gcgcgtgccc gatcacgccg tcgctcgtgc
      301 ccttatccgc gccgcgggtg gggccgtggc cgccaccagt gccaacgtct ccgggcagcc
      361 ggcggcgcgg tcggccggtg aggccctggc agcactggcc ggacgcgccg ccgccgtgct
      421 cgacgacggg ccatcgccgg gcggtcgcgc ctcaactatc gtcgattgca ctgtgcagcc
      481 gccagtcatc ctgcgcgtcg ggccgctatc ggccgctgat ctgggactga gggagtcatg
      541 agcggcctgc ttctgcccct cttgctcttt gtcgcggcgc tggcagcgac gttcctgacc
      601 tatcgcaaca tccgtcgggg tggagcgcgc ttctacacgc tggagcgcga gattatcctg
      661 cgccgggcgg ccttcaccct gatcatcggc gcgctcttct acaccggcgc gttgggcctg
      721 ctctactacc agcgccaaca gatgatcgaa gcggccgcgc cgccggaggg cacgcccgaa
      781 ggggatacgt ccgaaggcgc tgtcggcccc ggggtgacga cgccaacgcc cctcttcgag
      841 cagttcccgc ccacggccac ggccacttcg acgacgccgc agccgaccat cacgcccacg
      901 ccggtcacct gccgggctgt tgttgagggc agcggcgcaa gcggcatcta cctgcgcgac
      961 gccccccagg gcaccgagct ggtcatcctg gccgagggca cgctgctgac ggttctgcca
     1021 gacgagccgg tcgaggcggg cggctttctc tggcgcaagg tgcgggccgt gggcggcgaa
     1081 gagggctggg cggccgatga cttcctgacc atccgcgcgc cgtgtgccaa cgagtaagcc
     1141 gcttcgccgg cgaccccgcg ctatgatcat cggcattgac gccagccgcg ccgccgccgc
     1201 ccggcgcacg ggcacggaag cctacgccta ccagctcatc cgcgccctca tccccctggc
     1261 cgccgaacgc ggccacgggt tgcgcctgta tttcaacgac ccgccgccgg ccgatctctt
     1321 cccggcggcg gcgcacgtgg agatgcgggt catcccgctg cgccgcctgt ggacgcatgc
     1381 ccgcctcggt cacgaactgc ggacgcgccc gccggatgtt ttcttcaccc cggcccacgt
     1441 cattcccatc ggtgtgcgcg tgcccagtgt ggccaccgtc cacgacctgg gctatgagca
     1501 cttcccggag gcccacccct ggcggcagcg ggcctatctg cgttggagca cgcgccacaa
     1561 cgcccgcgcc agccgccgtg tactggtcga ttcgcgggcc acgggcgacg acctgacccg
     1621 cctctacggc acggccgcgg ccaagatcgc cgt
//
LOCUS       DAOSMQ010000616         1645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_74393_length_1645_cov_5.062893, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000616 DAOSMQ010000000
VERSION     DAOSMQ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74393_length_1645_cov_5.062893"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PK829_14765"
     CDS             complement(<1..299)
                     /locus_tag="PK829_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HOU42527.1"
                     /translation="MKNAIVELTRRPDVRAALAGYHRHLEEILAQIVAIQQVAAPTFA
                     EKRRAAYMEAQFLALVAAEVSHDALHNVFARLPGRDAGRPPLIISAHLDTVFPATT"
     gene            complement(296..1606)
                     /gene="hflX"
                     /locus_tag="PK829_14770"
     CDS             complement(296..1606)
                     /gene="hflX"
                     /locus_tag="PK829_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU42528.1"
                     /translation="MPTATRIHETAPPRERALMVGTALYDSEPLLTVEESLDELARLA
                     DTAGIDIVGQTMQRFEKPHSATYIGQGKVEEVKLLLEEMNADVVIFDDELLPRHQREL
                     EEVFGEDIKVIDRTALILDIFAQHAQTREGKLQVELAQLEYRLPRLTRMWTHLARQAG
                     GRAGGATGGVGLRGPGETQLETDRREIGRRIALIREELDAVRAHRERHRSKRQQTELQ
                     VVAIVGYTNAGKSTLLNRLSGADVYAADQLFATLDPTTRKVNMPGGREVLFTDTVGFI
                     QKLPTQIVAAFRATLEEIGEADMLLHVVDATHPNMAAQIEAVEDTLAELEVDHLPMVV
                     ALNKADALHDRAAVDALHLSVPAALVSARTGKGIDELLTLVEATMVGLLQPIRVHIPY
                     ERGDLLSLFYERGQVDSESHSGSGVQLYGRLPPRLLPVFANYTV"
BASE COUNT          253 a          518 c          577 g          297 t
ORIGIN      
        1 gtcgtcgccg ggaagacggt atccaggtgg gcggagatga tcagcggcgg ccggccggcg
       61 tcgcggcccg gcagacgggc gaagacgttg tggagcgcgt cgtggctcac ttcggccgcg
      121 accagggcga ggaattgggc ctccatgtaa gccgcgcggc gcttctcggc gaaggtcggc
      181 gcggccacct gctggatggc gacgatttgg gccagtatct cctccaggtg gcggtgatag
      241 ccggccaggg cagcgcgcac gtccggccgg cgggtgagtt cgacaatggc gtttttcata
      301 cggtgtagtt ggcaaagacg ggcaacaagc gtggcggcag ccggccatag agctggacgc
      361 ccgagccgct gtggctttca ctatccacct ggccacgctc gtagaacaga gacagcaggt
      421 cgccgcgctc gtaggggatg tggacgcgaa taggctgcaa caagccgacc atcgtcgctt
      481 ccaccagcgt cagcagttcg tcgatgccct tgcccgtgcg cgccgagacg agcgccgcgg
      541 gcacactcag gtgcagcgcg tcgacggcgg cgcggtcgtg cagcgcgtcg gctttgttca
      601 gggccacgac catgggcagg tgatcgactt ccagctcggc cagcgtgtcc tcaacggcct
      661 cgatctgcgc ggccatgttg gggtgcgtcg cgtcgacgac gtggaggagc atatcggcct
      721 cgccgatctc ctccagcgta gcgcggaaag cggcgacgat ctgggtgggc aacttctgaa
      781 tgaagccgac agtgtcggtg aagagcactt cccggccgcc gggcatgttg accttgcgcg
      841 tcgtcgggtc gagcgtggca aagagctggt cggcagcgta gacgtcggcc ccgctgaggc
      901 ggttgagcaa cgtcgatttg ccggcgttgg tgtagccaac gatagccacg acttgtagct
      961 ctgtctgctg ccgcttggag cggtggcgtt cgcggtgggc gcggacggcg tccagttcct
     1021 cgcggataag ggcgatgcgc cggccaatct cacgccggtc ggtctccaac tgcgtttcgc
     1081 cggggccgcg caggcccacg ccgccggtcg cgccgccggc ccggccgccg gcctgccgcg
     1141 ccaggtgggt ccacatgcgc gtcagccgtg gcaagcggta ctccaactgg gccaactcga
     1201 cttgcagctt accctcgcgc gtctgggcgt gctgggcgaa gatgtcgagg atgagcgccg
     1261 tccggtcgat gaccttaatg tcctcgccga agacctcttc caactcgcgt tggtgacgcg
     1321 gcagcagttc gtcgtcgaag atgacgacat cggcgttcat ctcctctagc agcagcttga
     1381 cctcctcgac tttgccctgg ccgatgtagg tagcgctgtg gggcttttca aagcgctgca
     1441 tcgtctggcc gacgatgtcg atgccggccg tgtcggccag ccgggccagt tcgtcaaggc
     1501 tctcttcaac tgtcagcagc ggctcgctgt cgtacagtgc cgtgccgacc atcagcgccc
     1561 gctcgcgcgg cggggccgtt tcgtggattc tggttgccgt gggcataaga ccctctccct
     1621 aaccctctcc cagagggaga gggaa
//
LOCUS       DAOSMQ010000617         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_74668_length_1641_cov_5.438840, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000617 DAOSMQ010000000
VERSION     DAOSMQ010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74668_length_1641_cov_5.438840"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK829_14775"
     CDS             complement(<1..203)
                     /locus_tag="PK829_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42529.1"
                     /translation="MKKLNRGVAVVGTGMSQFGAFPEKTTRDLFVEAFQDMRASVGRG
                     LDPTDIEAIYIGNYSSDLFEGQG"
     gene            complement(219..>1641)
                     /locus_tag="PK829_14780"
     CDS             complement(219..>1641)
                     /locus_tag="PK829_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU42530.1"
                     /translation="ALENSELVRRETLIYDIPAEQNRAATVREEEAVYLPALYFSETG
                     LTRQVRRLIEHPTSRLRGAGRRATGVPAVAGGPALTAQQQQAVQATLAHKLTILTGGP
                     GTGKTTTLRTLLDTLDAGGHTYVLASPTGRAAKRLTEATGREAKTIHRLLEFKPGEGF
                     GRGERNPIDADLIVIDEASMLDLVLAYNLLRAIGSDSHLLLVGDIDQLPSVGAGDVLR
                     DLIASDVAAVVRLETIFRQAADSLIIRNAHRINQGLMPETHADAQDFFLFIKDEPEEA
                     AALLVDVVAQRIPHKFGLDPLDDVQVLAPMYNGAAGVTRLNTILQETLNPPGSRKVEK
                     RLGGRIFRVGDKVMQTVNNYDKSVYNGDIGRVTAIDLIGQTLTVSIDGAPVVYDFLEA
                     DELMHAFAVSVHKSQGAEYPCVVIPVVVQHYLMLQRNLLYTAVTRARRLVVLVGTRRA
                     IQIAVRTNTVVQRHTALDWRLKH"
BASE COUNT          248 a          545 c          553 g          295 t
ORIGIN      
        1 tggccctgcc cctcgaagag gtcgctgctg tagttgccga tgtagatcgc ttcgatgtcg
       61 gtggggtcaa gaccgcggcc gaccgaggcg cgcatatcct ggaacgcttc gacaaacagg
      121 tcgcgcgtcg tcttctccgg gaaggcgcca aactggctca tccccgtgcc gaccacggcg
      181 acgccacgat tgagtttctt catggcagtc tccttgcgtt aatgcttcaa acgccaatcc
      241 agcgccgtgt ggcgctgcac cactgtgttg gtgcgcacgg cgatctggat ggcccggcgc
      301 gtgcccacca gcaccaccag ccggcgcgcc cgcgtcaccg ccgtatagag caggttgcgt
      361 tgcagcatca ggtagtgctg caccacgacc gggatgacca cgcacggata ctccgccccc
      421 tgcgacttgt ggacgctgac ggcgaaggcg tgcatgagtt cgtccgcttc caggaagtcg
      481 tagaccaccg gcgcgccgtc gatgctgact gtcaacgttt gtccaatgag gtcgatggcc
      541 gtcacccgcc cgatgtcccc attatagacg cttttgtcgt agttgttgac cgtttgcatc
      601 accttgtcgc cgacgcgaaa gatacggccg ccgaggcgct tctctacctt gcggctgccg
      661 ggcgggttga gcgtctcctg caagatcgtg ttcagccgcg tcacgccggc cgcgccgttg
      721 tacatggggg ccagcacctg cacgtcgtcg agcgggtcga ggccgaactt gtggggaatg
      781 cgctgggcga cgacatcgac cagcagcgcc gccgcctcct ccggctcgtc cttgatgaac
      841 aggaagaagt cttgcgcgtc ggcgtgggtt tccggcatca ggccctgatt gatgcggtgg
      901 gcgttgcgga taatgagcga gtcggccgcc tggcgaaaaa tcgtctccag gcgcaccaca
      961 gcggccacgt cggaggcgat caggtcgcgc agcacgtcac ccgcgcccac agacggtagc
     1021 tggtcgatgt cgccgacgag cagcaggtgg ctgtcggagc caatggcccg cagcaggttg
     1081 taggccagca ccaggtcgag catcgacgct tcgtcgatga cgatcaggtc agcgtcgatg
     1141 gggttgcgct cgccacggcc aaagccctcg cccggcttga actccagcag gcggtggatg
     1201 gtcttggcct cgcggccggt ggcctcggtc agccgcttgg cggcccggcc ggtgggcgag
     1261 gccagcacat aggtgtggcc acccgcgtcg agtgtgtcca gcagcgtgcg cagggtcgtc
     1321 gtcttgcccg tgcccggccc gccggtcagg atggtcagct tgtgggccaa cgtggcctgg
     1381 acggcctgtt gctgctgggc cgtcagggcc gggccaccgg cgacggccgg cacgccagtc
     1441 gcccgccgcc ccgccccgcg cagccggctg gtgggatgct cgatcagccg ccgcacctgc
     1501 cgcgtcaggc ccgtttcgga gaagtagagc gcgggcaggt agacggcctc ctcctctcgc
     1561 acggtcgcgg cgcggttctg ctcggcgggg atgtcgtaga tcagcgtctc ccggcgtacc
     1621 agctcactgt tctccagcgc c
//
LOCUS       DAOSMQ010000618         1640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_74738_length_1640_cov_3.129968, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000618 DAOSMQ010000000
VERSION     DAOSMQ010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74738_length_1640_cov_3.129968"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PK829_14785"
     CDS             complement(<1..360)
                     /locus_tag="PK829_14785"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU42531.1"
                     /translation="MTESVIIIGAGIGGICSAIHLAQQGLHVTVVEKNNRPGGRCDRI
                     SREGHHFDTGPTLLVMPLLYEEEFARLGVPMREALDLTRVDPTYHLVFDDGSRLNLTS
                     DLKRMYDQLEAIEPGAFD"
     gene            complement(439..888)
                     /locus_tag="PK829_14790"
     CDS             complement(439..888)
                     /locus_tag="PK829_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42532.1"
                     /translation="MLRDTAEDAALGYINVPAELLTANGLLTVNGLLTVNGLDVGDLG
                     AVLFDRPAFREWVAARVALARRDFAIGRRLIEGLACRRCRLAGYAYIARFEYVAGLIE
                     RDGYRLRAAYPERQSRAAALWMARRTAAGILRPRRQDNGALRPAMER"
     gene            complement(825..1397)
                     /locus_tag="PK829_14795"
     CDS             complement(825..1397)
                     /locus_tag="PK829_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42533.1"
                     /translation="MSSILAPVDLAPAPTRDHADLARSITRAASTQTYGTIRLLADRD
                     RAADAYRAYAYFRWVDDQLDDGTALPAERAAFLARQQALLNAGYAGRSLPAGLAAEET
                     LLAELIAADTEPDSGLRSYLGHMMAVMAFDVARPRRPVSRRATSKAMTASRRATRPVT
                     PPSAAPTSSTCCAIRPKTRPWATSTCRPSF"
BASE COUNT          252 a          528 c          605 g          255 t
ORIGIN      
        1 gtcgaaggcg cccggttcga tggcctcgag ctggtcgtac atgcgcttca ggtccgacgt
       61 caggttcagc cgcgagccat cgtcgaaaac caggtgatag gtgggatcga cgcgggtcag
      121 gtcgagcgcc tcgcgcatgg gcacgcccag ccgggcgaac tcctcctcgt agagcagggg
      181 catgaccagc agcgtcgggc cggtgtcaaa gtggtggccc tcgcggctga tgcggtcgca
      241 acggccgccg ggccggttgt tcttctccac gacggtgacg tgcagcccct gttgggccag
      301 gtggatggcc gagcaaatgc cgccgatgcc ggcgccgatg atgataacgg attcggtcat
      361 ggtgactcct tgtctgtcaa tcagaatggc agcacggccc atctcgcggg cgggccgcgc
      421 tgccgccccc gccgggggct atctctccat cgccgggcga agcgcgccgt tgtcctggcg
      481 tcgcgggcgc aagatgccgg cggctgtacg ccgggccatc catagcgccg ccgcgcggga
      541 ctggcgctcc gggtaagctg cgcgcagacg atagccgtcg cgctcgatca gaccggccac
      601 gtactcgaag cgggcgatgt aggcgtagcc cgccagccgg caacggcggc aggccagccc
      661 ctcgatgagc cgccgcccga tggcgaagtc gcgccgcgcc aacgccaccc gcgccgccac
      721 ccactcgcgg aaggcgggcc ggtcgaacag taccgcgccc aggtcgccca cgtccagccc
      781 gttgacagtc aggagaccgt tgacggtcag gagaccgttg gcggtcagaa gctcggccgg
      841 cacgttgatg tagcccaggg ccgcgtcttc ggccgtatcg cgcagcatgt ggatgatgtg
      901 ggcgccgcgg agggcggcgt aacgggccgg gtcgcgcggc ggctggcagt catggccttc
      961 gacgtggccc ggcgggagac agggcggcgc ggccgggcca cgtcgaaggc catgacggcc
     1021 atcatgtgac ccaggtaaga gcgcaggccg ctgtccggct ccgtgtcggc ggcgatcaac
     1081 tcagccagca gcgtctcctc cgcggccagg ccggccggca gggatcggcc ggcgtagccc
     1141 gcgttcagca gcgcctgttg ccgggccagg aaggcggcgc gctcggcggg cagggccgtg
     1201 ccgtcgtcca gttggtcatc gacccagcgg aagtaggcgt aggcgcgata ggcgtcggcc
     1261 gcccggtcgc ggtcggccag caggcggatg gtgccatagg tctgcgtgct ggcggcgcgg
     1321 gtgatcgacc gggccaagtc tgcgtggtcg cgagtgggcg ctggggccag atcgaccggg
     1381 gccagaatac ttgacataac gactcatcgg tgcggggtgt gtcccgttgg gtcaggatgt
     1441 ggccggcggc gtcgggtaga gaagcgcacc tacaccatta gcaagcataa tcggccgcgc
     1501 cggcgctgta tagtgacaaa gatcaccttt ttcagactct gttttgtggc gcgattctcc
     1561 gaatcgctct tctcttccaa acacgtggcg cgatttgggg aatcatgccc ggggcaacgg
     1621 ctgcgcggcg tgcgattcgg
//
LOCUS       DAOSMQ010000619         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_75529_length_1627_cov_4.337786, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000619 DAOSMQ010000000
VERSION     DAOSMQ010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75529_length_1627_cov_4.337786"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1178
                     /locus_tag="PK829_14800"
     CDS             90..1178
                     /locus_tag="PK829_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU42534.1"
                     /translation="MTNPNIQRALAEQAYAEELAELARNDRRPRPPNWRLSPWAVAAY
                     VLGATLDNGFVISPKYIGNARVIEIAIATLTTDRALLLLGVPGTGKTWVSEHLAAAVS
                     GDSTLLVQGTAGTSEEVIRYGWNYASLIARGPSLDALVPSPVLTAMRDGKIARVEELT
                     RIPSDVQDALITILSEKLLPIPELGQAVQAVRGFNVIATANDRDRGINDLSSALKRRF
                     NTVVLPLPATAEDEVTIVSRRVADLSHALELPAELPAAEEIRRVVTIFRELRAGLTED
                     GRAKVKSPSGTLSTAEAISVVNSGLALAAHFGDGRLAPGDLAAGLTGAIVKDPVQDAV
                     IWREYLETVMAERDDWAALYEACREMAA"
     gene            1175..>1627
                     /locus_tag="PK829_14805"
     CDS             1175..>1627
                     /locus_tag="PK829_14805"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HOU42535.1"
                     /translation="MSGATLRVFGVRHHGPGSARAVRAALAGYAPDCVLVEGPPDADA
                     LIPWLAHPALELPTALLVYCPDEPRRATFYPFATFSPEYRALRYALEHDIAVGFIDLP
                     RRHMLAADVAPAMPSAEMFNRLAAAAGHAAYEPWWNEAVEQRREAGELF"
BASE COUNT          245 a          583 c          548 g          251 t
ORIGIN      
        1 cctttttgtc cttggcctta gcctccatca ttggttaagg tagcaacttg agtgtctact
       61 gtctactgac ctactgaccc cggagaccca tgaccaaccc caacatccaa cgcgccctgg
      121 ccgaacaggc ctatgccgaa gaactggccg aactggcccg caacgaccgc cggccgcggc
      181 cgcccaactg gcgcttgtcg ccctgggccg tggccgctta cgtgctgggg gcgacgctcg
      241 acaacggctt cgtcatcagc cccaagtaca tcggcaacgc ccgcgtcatc gagatcgcca
      301 ttgccacgct gacgaccgat cgggcgctgc tgctgctcgg cgtgccgggc acgggcaaga
      361 cgtgggtgtc ggagcacctg gccgcggccg tctccggcga ctcaacactc ctggtgcagg
      421 gcacggccgg cacgagcgag gaagttatcc gctacggctg gaactacgcc agcctcatcg
      481 cccgcgggcc gagcctcgac gccctcgtgc ccagccccgt gctaacggcc atgcgcgacg
      541 gcaagatcgc ccgcgtggag gagttgacgc gcatcccgtc tgacgtgcag gacgcgctca
      601 tcaccatcct gtcggagaag ttgctgccca tcccagagtt ggggcaggcg gtgcaggcgg
      661 tgcgtggttt caacgtcatc gccacggcca acgaccgcga ccggggcatc aacgacctgt
      721 ccagcgctct caagcggcgc ttcaacaccg tggtgctgcc cctgccagca acggcggagg
      781 acgaggtgac catcgtcagc cgtcgcgtgg ccgacctgag ccatgcgctg gaactgccgg
      841 ccgagctgcc cgcggccgag gagattcgcc gcgtggtgac catcttccgc gagctgcgcg
      901 ccgggttgac cgaggacggc cgggccaaag tcaagtcgcc cagcggcacg ctgagcaccg
      961 ccgaggccat ctccgtggtc aatagcggcc tggccctggc ggcccacttc ggcgacggcc
     1021 ggctggcccc cggcgacctg gccgcggggc tgaccggagc catcgtcaag gacccggtgc
     1081 aggacgccgt catctggcgc gagtatctgg agacagtcat ggccgagcgc gacgactggg
     1141 cggcgctgta cgaagcctgc cgggaaatgg ccgcgtgagc ggggccaccc tgcgagtctt
     1201 cggcgtgcgc caccacgggc cggggtcggc gcgggcggtg cgggcggcgc tggccggcta
     1261 cgcgcccgat tgtgtgctgg tggaggggcc gcccgacgcc gatgcgctca tcccctggct
     1321 ggcccatccg gcgctggagt tgcccactgc tctgctggtc tactgccccg acgagccgcg
     1381 ccgggcgaca ttctatccct ttgccacttt ctcgccggag taccgggcgc tgcgctacgc
     1441 cctggagcac gacattgccg tgggcttcat cgacctgccc cgccgccaca tgctggccgc
     1501 cgacgttgcc ccggccatgc cctcggcgga gatgttcaac cggctggccg cggccgccgg
     1561 ccacgctgcc tatgagccgt ggtggaatga ggccgtcgag cagcggcgcg aggcgggcga
     1621 actcttc
//
LOCUS       DAOSMQ010000620         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_75982_length_1620_cov_4.699681, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000620 DAOSMQ010000000
VERSION     DAOSMQ010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75982_length_1620_cov_4.699681"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PK829_14810"
     CDS             complement(<1..1113)
                     /locus_tag="PK829_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOU42536.1"
                     /translation="MSTEIVVPELGESVVEATVGEWRVAEGQAVQVGDVVVELETDKV
                     DVEVGATAAGVLSRILHPAGSDVRIGDVLGLIDSAGGAAPATTTPSVNDQTARPDAPV
                     QASATPLPTDRERATPVARRMAEAEGVDLANVPGSGTGGRVTKQDVAERLGPAADRGR
                     PTADGRQTTDDDPQTTAAGPTAPRPSNAPAPLLARPPASQAGARPEERQRMSRRRRTI
                     AQRLVEAQHTAAMLTTFNEVDMSAVMDMRRRRQEAFVNAHGIKLGLTSFFVKACIGAL
                     RAFPRLNAEIQGDEIVLKGYYDIGIAVGAEEGLVVPVIRDADRLSFAAIERQVRDFAA
                     KAQDNTFTLEDLRGGTFTITNGGVFGDREGDAAQVFQ"
     gene            complement(1110..>1620)
                     /locus_tag="PK829_14815"
     CDS             complement(1110..>1620)
                     /locus_tag="PK829_14815"
                     /inference="COORDINATES: protein motif:HMM:NF028180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOU42537.1"
                     /translation="PLIVMSPKSLLRNARVYSSPRDLAEGHWRPVIDDAQAVAGRAAV
                     RRLILCSGKVYVDMSADPRRADCRDVAIARVEQLYPWPEAELNEVIEGYPDAREIVWL
                     QEEPTNAGAWEFVQPRLLRQLGGRLPLRLIARPRRASPAEGTMAMHIFSQTGLLDRAY
                     EVNEEMTTP"
BASE COUNT          226 a          562 c          564 g          268 t
ORIGIN      
        1 ctggaagacc tgcgcggcgt caccttcacg atcaccaaag acgccgccat tggtgatcgt
       61 gaaggtgccg ccgcgcaggt cttccagcgt aaaggtgttg tcctgggcct tggcggcgaa
      121 gtcgcgcacc tggcgctcga tggccgcgaa cgacagccgg tcggcgtcgc ggatgacggg
      181 cacgaccagc ccctcctccg cgccgacagc aatgccgatg tcgtagtagc ccttcagaac
      241 gatctcgtcc ccttgaatct cggcgttgag tcgcgggaag gcgcgcagcg cgccgatgca
      301 agccttaacg aagaacgacg tcagccccag cttgatgccg tgggcgttga cgaaggcctc
      361 ctgccgtcgc cggcgcatgt ccatgaccgc gctcatatcg acttcgttaa acgtcgtcag
      421 catggccgcg gtgtgctggg cctccaccag acgctgggcg atggtgcggc ggcggcgaga
      481 catgcgctgg cgctcctccg ggcgcgcgcc ggcctgggaa gcaggggggc gggcgagtag
      541 gggggcaggg gcattggagg gccggggtgc ggtgggtccg gcggctgtcg tctgtgggtc
      601 gtcgtccgtc gtttgtcggc cgtccgcggt cggccgtccg cggtcggccg ccggccccag
      661 ccgctcggcc acatcctgct ttgtgacccg gccaccggtg ccgctgccgg gcacgttggc
      721 cagatcgacc ccctcagcct cagccatgcg gcgggcgacg ggcgtggccc gttccctatc
      781 ggtgggcaga ggtgttgcgc tcgcctgaac tggcgcgtcc ggccgggctg tctggtcatt
      841 gacagatggg gtggtggtcg ccggcgcggc tccaccggcg ctgtcaatga ggccgagcac
      901 gtcaccgatg cgcacgtcgc tgccggcggg gtgcaggatg cggctcagca cgcccgcggc
      961 cgtggcccca acctcaacat cgaccttgtc cgtctctaac tcgacgacca catcgccaac
     1021 ctggacggcc tgcccctcgg ccacgcgcca ctcgccgaca gtcgcctcga ccactgattc
     1081 gcccagttcg ggcacgacga tctctgtgct catggggtcg tcatctcctc gttgacttcg
     1141 taggcccgat ccagcagccc cgtctggctg aagatgtgca tcgccatcgt accctcggcc
     1201 gggctggcgc ggcgcggccg ggcgatgagc cgcagtggca gccggccgcc caactggcgc
     1261 agcagccgcg gctggacgaa ctcccacgcg ccggcattgg tcggctcctc ttgcagccag
     1321 acaatctcgc gggcgtctgg atagccctcg atcacctcat ttagctccgc ctccggccag
     1381 ggatagagtt gctccacgcg ggcgatggcc acgtcgcggc agtcggcccg acgcgggtcg
     1441 gcgctcatat cgacgtagac cttgccgctg cacaggatca ggcggcgtac ggcggcgcgg
     1501 ccggccaccg cctgagcgtc gtcgatgacc ggccgccagt gcccttcggc caggtcgcgt
     1561 ggcgacgagt agacccgcgc gttgcgcagc aggcttttgg gcgacatgac gatcaggggc
//
LOCUS       DAOSMQ010000621         1617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_76171_length_1617_cov_3.861716, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000621 DAOSMQ010000000
VERSION     DAOSMQ010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76171_length_1617_cov_3.861716"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="PK829_14820"
     CDS             <1..1592
                     /locus_tag="PK829_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU42538.1"
                     /translation="RATDAGFNRADWATATAKRTLVDGAPPTSTVNVITPVDAATVQV
                     SWRGSDFGSGVATYDVWVRQGTAGDWTRWQRATTALSAEFTGQTGQTYYFRSQAIDRA
                     GLREPWRPSPQAQTTLGAPDAPAGPATPTGTVRVLLPLVTAVAGSANAQDMLSIADWP
                     TLGGNAGHTGVNAGDPGASRYARVWSTELGANGEEMPQQVTSANGLVLALVDASWRSG
                     SHPRVVALDVETGIPAWGFESALSALTHPPAIAHGAVYFGQDTYTYDLLAVDLRTGHQ
                     IWAGVGFCEADNYRFQHPLVVGNRIFLGIARGLDAIAAASGQSAWHFSDHCYTNGSDG
                     WAAAYYGGRLYYQPGGYLVELDANTGQTLRATNLYGDYWRGTIAAPVVTADTIVSVAY
                     DLTVLERGTHQLAWTIQGQTEDYYAGGTMPAVAGRVVYALRANTLRAYNLSNGVELWR
                     FTADGPLLAAPIVAGQYVYVASATHTYVLNRQTHLVEWDTPTGGILSIANGYLFIAET
                     IYDTMDYPQKAILHAYRAQEP"
BASE COUNT          283 a          558 c          511 g          265 t
ORIGIN      
        1 gccgggctac cgatgccggc ttcaaccgcg ccgattgggc cacagcgacg gcgaaacgca
       61 ccctggtcga cggcgcgccc ccaacctcca cggtgaacgt catcacgccg gtcgatgccg
      121 ctaccgtcca ggtaagctgg cgtggttccg acttcggcag cggcgtggca acctacgacg
      181 tctgggtgcg ccagggcaca gccggcgatt ggacgcgctg gcagcgagcg acaaccgccc
      241 tgagcgccga gttcaccggg cagaccggcc agacctatta cttccgcagc caggccatcg
      301 atcgcgccgg cctgcgcgag ccgtggcggc cgtcgcccca ggcccagacg acgctcggcg
      361 cgcccgacgc gccggccggc ccggccacgc caaccgggac tgtgcgcgtt ttgctgccgc
      421 tggtgaccgc cgtcgctggc tcggccaacg cgcaggacat gctctcgatt gccgactggc
      481 cgacgctggg cggcaacgcg ggccacacgg gcgtcaacgc cggcgaccca ggcgcgtcgc
      541 gctacgcgcg cgtctggtcg accgaattgg gcgcaaacgg agaggaaatg ccgcaacaag
      601 tcaccagcgc caacggtctg gtgctggcgc tggtcgatgc cagttggcgc tcgggcagcc
      661 acccccgcgt cgtcgcgctt gacgtggaga cgggcattcc ggcctggggt tttgaatcgg
      721 cgctctctgc cctgactcac ccaccggcaa ttgctcatgg cgcggtttat ttcggccagg
      781 acacatatac gtatgacctt ctggccgtcg atctacgcac tggtcaccaa atctgggccg
      841 gagttggctt ttgtgaagcc gataattatc gcttccagca tccgctggtc gtcggcaatc
      901 gcatcttcct gggcattgct agagggctgg atgcgatcgc ggcagccagc ggccagtcgg
      961 cctggcactt ttcagaccat tgctacacca atggcagcga tggctgggcg gcggcgtatt
     1021 acggcggccg cctctactac cagccgggcg gctacctcgt cgaactggat gccaacaccg
     1081 gccagacact ccgcgcgact aacctgtacg gcgactactg gaggggcact atcgccgcgc
     1141 ccgttgtcac cgcggacacc atcgtcagcg tcgcctacga cctgacggtt ctggaacggg
     1201 gaacgcacca actcgcctgg acgatccagg gccagacaga agattactac gcgggcggca
     1261 cgatgcccgc cgtcgccggc cgcgtcgtct acgcgttgcg cgcgaacacg cttcgggcct
     1321 acaatctgag caacggcgtg gaactgtggc gcttcacggc cgatgggcca ttgttggccg
     1381 cgccgatcgt cgccggccag tatgtctacg tggcctcggc cacccacacc tacgtgctga
     1441 accggcagac gcacctggtc gagtgggaca caccgaccgg cggcatcctg tcaatcgcca
     1501 acggctatct attcatcgcc gagacgatct acgatacgat ggactatccg caaaaagcca
     1561 tactccacgc ctaccgggcg caggagccat agtctgaatt gtggagggcc tgatgca
//
LOCUS       DAOSMQ010000622         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_76296_length_1615_cov_3.666026, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000622 DAOSMQ010000000
VERSION     DAOSMQ010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76296_length_1615_cov_3.666026"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK829_14825"
     CDS             <1..435
                     /locus_tag="PK829_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42539.1"
                     /translation="PGERWVTVGTDDDVPRLVGLLERISREVANSDVDLSVFSAVDSP
                     VLRWYFRAFERFESGPALPIDSQADVVITAGDSEPQLPNDYFGADFGLEQHEVTGVGL
                     TSEGGGAVVDQVLRAWLFHHSSVPVEQQRIVVWVRSDLATAK"
     gene            446..1057
                     /locus_tag="PK829_14830"
     CDS             446..1057
                     /locus_tag="PK829_14830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU42540.1"
                     /translation="MTEQPSFTYRSFDHDSPEPFSPDETLLLIQRCLAGEQPAYVALY
                     DQFSGIIYRLAYGLLQHREDAEEVLQDSFEYAFRRLDHYDARKSAFKTWLYRIAVSRC
                     HNKRRRKWLPSLSLQQLVEVDVRDPAAAPPDERVALSEQQRAVWQAVGALSPKLREVA
                     ILRYYGGLQYGEIGAVLGIPTKTAESRMRLAHKTLREQLSDLA"
     gene            1103..>1615
                     /locus_tag="PK829_14835"
     CDS             1103..>1615
                     /locus_tag="PK829_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOU42541.1"
                     /translation="MDSVPMDIIHVHQLIPDYVLGLLSADDSHRVEQHARQCPLCRQA
                     IQRERQLEFRVRGAVQLAARPPAGRLEALRPAFPRGATRVRGPLYRQFAPAMALVMLL
                     VVVLLAQVGGVGLPAPGFARATKTPTSTATPLPTATLAATAGPSTTAAAAALTRVNWP
                     NASSPAPEPAP"
BASE COUNT          259 a          558 c          544 g          254 t
ORIGIN      
        1 cccggcgagc gctgggtgac ggtgggcacg gacgacgatg tgccgcggct ggtggggctg
       61 ctggagcgca tctcccgcga agtggccaac tctgacgtcg acctgagcgt cttcagcgcc
      121 gtcgattcgc ctgtgttgcg ctggtacttc cgcgccttcg agcgcttcga gagcggcccg
      181 gcgctgccca ttgattcgca ggcggacgtg gtcatcaccg ccggcgacag cgagccacaa
      241 ctgcccaacg actacttcgg ggccgacttc gggctggagc agcacgaagt gacgggcgtg
      301 ggccttacca gcgagggcgg cggcgcggtt gtcgaccagg tgttgcgcgc ctggctgttc
      361 caccacagca gcgtacccgt ggagcaacag cgcatcgtcg tctgggtgcg ctcggacctg
      421 gccacggcga aatgaagggc gcacgatgac cgagcaaccg tcgttcacct accggtcgtt
      481 tgaccacgat agcccggagc cattctcgcc ggacgagacg ctgctactca tccagcgctg
      541 cctggccggg gagcaaccgg cctatgtcgc cctctacgac cagttcagcg gcatcatcta
      601 ccggctggcc tatggcctct tgcagcaccg cgaggacgcc gaagaagtct tgcaagacag
      661 tttcgagtac gccttccggc gtctcgacca ctacgacgcg cgtaagtcgg ccttcaagac
      721 gtggctctat cgcatcgccg tcagccgctg ccacaacaag cggcggcgca agtggctgcc
      781 gtcgctgtcg ctgcaacaac tggtcgaggt agacgtgcga gacccggccg ccgccccacc
      841 cgacgaacgc gtcgctctca gcgagcagca gcgggcggtc tggcaggccg tcggcgcgct
      901 gtcgcccaag ctgcgcgagg tggccatcct acgctactac ggtggcctac aatatggcga
      961 aatcggcgcg gtgctgggca ttcccaccaa gacggccgag tcgcgcatgc ggctggccca
     1021 taagacgttg cgcgagcaac tcagcgatct ggcatagcgc ctctgggcga catggcgtgt
     1081 atagatgaat taggaattca gaatggatag cgtcccgatg gatatcatcc acgtccacca
     1141 actgattccc gactatgtac tggggctgct gagcgccgac gacagccacc gcgtggagca
     1201 acacgcccgg cagtgcccgc tgtgccgcca ggccatccag cgcgagcggc agttggagtt
     1261 tcgcgtgcgc ggggcggtgc aactggccgc gcggccgccg gccggccggc tggaggcgct
     1321 gcgaccggcc ttcccacggg gggcgacgcg cgtgcgcggc ccgctctacc gccagttcgc
     1381 gccggcgatg gccctggtca tgctgctggt ggttgtgctg ctggcgcagg tgggcggcgt
     1441 tggcctgccc gcgcccggtt ttgcccgcgc gaccaagacc ccgaccagca cggcgacgcc
     1501 gctgcccacg gccacgctgg cagctactgc cggaccgagc acgacggccg cggcggcggc
     1561 gctgacgcgc gtcaactggc caaacgcctc ttcgcccgcc cccgaacccg cgccg
//
LOCUS       DAOSMQ010000623         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_76434_length_1613_cov_2.878049, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000623 DAOSMQ010000000
VERSION     DAOSMQ010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76434_length_1613_cov_2.878049"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1613)
                     /locus_tag="PK829_14840"
     CDS             complement(<1..>1613)
                     /locus_tag="PK829_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU42542.1"
                     /translation="TLFVDEVHRWNKAQQDALLPHVENGTIILIGATTENPYFEVNKA
                     LVSRSRIFQLRPLTEDDLRAVARQALSDPERGYGKLNVALSADALDHLVDVANGDARG
                     VLNALELAVETTPPDDNGVISVDLAVAEESIQRRAVLYDKDGDVHYDTISAFIKSLRG
                     SDADAALYWMARMVYAREDPRFIFRRMAIFAGEDVGMADPAAMGVVVNAWETFERIGM
                     PEGRFPLALAALYLATAPKSNSAFAFFDALGAVEKEREADIPNHLKDGNRDKEGFGHG
                     EGYLYPHAYRDHWVAQQYLPEALQGKVFYQPGEIGYEQRIRDEVARRREAQLAAMVES
                     ADDPHLEVLTTSPRDRGKDAWLQRAVAGSGRQLAALRDRLFEMAAVQRHHLVLDVNAG
                     SGLLTWEAARRAPEGGVWALTADATAGEALRQQAARLPEVERPVILIGALDELDYLLR
                     LRGEEAVRFDRILGRNILPVWRDFPNRAFATLATRLLPGGRLVLAQPIPRHTQRLYEL
                     VSWGRATALRDKVAAAEEAIYADTRDPLV"
BASE COUNT          232 a          554 c          572 g          255 t
ORIGIN      
        1 gaccagtgga tcgcgggtgt cggcgtagat ggcctcttcg gcggcggcca ccttatcgcg
       61 cagcgccgtc gcccggcccc aactcacaag ctcgtagagc cgctgggtgt ggcggggaat
      121 cggctgggcc aggacgagac ggccgcccgg caagaggcgc gtcgccagcg tagcgaaagc
      181 gcgattggga aaatcgcgcc acaccggcag tatgttgcgg ccgaggatgc ggtcgaagcg
      241 caccgcctcc tccccgcgca ggcgcagcag gtaatccagt tcgtcaagcg cgccgatgag
      301 gatgaccggc cgctccacct ccggcaggcg cgccgcctgc tggcgcagcg cctcgccggc
      361 ggtcgcgtcg gccgtcagcg cccagacgcc gccctccggc gcgcgccggg ccgcctccca
      421 cgtcagcagc ccgctgccgg cgttcacgtc cagcaccagg tggtggcgct ggacggcggc
      481 catctcgaac agccggtcgc gcaacgccgc cagttgccgg ccgctgccgg ccacggcccg
      541 ttgcagccag gcatccttgc cgcgatccct ggggctggtg gtcagcactt ccagatgggg
      601 gtcgtcggcg ctctcgacca tcgctgccag ctgcgcctcg cgccgccggg ccacctcgtc
      661 gcggatgcgc tgctcgtagc cgatctcgcc cggctggtag aagaccttgc cttgcagcgc
      721 ttcgggcaag tactgctggg ccacccagtg atcgcggtag gcgtgggggt agaggtagcc
      781 ctcgccgtgg ccaaagccct ccttgtcgcg gttgccgtcc ttcagatggt tggggatgtc
      841 ggcttcgcgc tccttctcca ccgcgcccag cgcgtcgaag aaggcgaagg cagaattgga
      901 cttgggggcc gtggccagat agagcgccgc cagagccagc ggaaagcgcc cctccggcat
      961 gccgatgcgc tcgaacgtct cccaggcgtt gacgaccacg cccatcgccg ccgggtcggc
     1021 catgccgacg tcctcgccgg caaagatggc catgcgccgg aagatgaagc gcggatcctc
     1081 acgggcatag accattcggg ccatccagta gagagccgcg tcggcgtcgg agccgcgcag
     1141 gctcttgata aaggccgaga tggtgtcgta gtggacgtcg ccgtccttgt catagagcac
     1201 ggcccggcgc tggatcgact cctcggctac ggccagatcg acgctgatga cgccgttgtc
     1261 gtccggcggc gtcgtctcca cggccagctc cagcgcgttg agcacgccgc gggcgtcgcc
     1321 gttggccacg tccactaggt ggtcgagcgc gtcggccgac agggcgacgt tgagcttgcc
     1381 atagccgcgc tccgggtcgc tcagcgcctg gcgggcgacg gcgcgcaggt cgtcctcggt
     1441 cagcggccgt agctggaaga tgcggctgcg gctgacgagg gccttgttga cctcgaagta
     1501 ggggttctcc gtcgtcgcgc cgatgaggat gatcgtgccg ttctcgacgt ggggcagcag
     1561 ggcgtcctgc tgggccttgt tccagcggtg gacttcatcg acgaagaggg tcg
//
LOCUS       DAOSMQ010000624         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_77838_length_1592_cov_3.152245, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000624 DAOSMQ010000000
VERSION     DAOSMQ010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77838_length_1592_cov_3.152245"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PK829_14845"
     CDS             complement(<1..490)
                     /locus_tag="PK829_14845"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HOU42543.1"
                     /translation="MKRLGKVLLWIVVAVVGVVLLALVVAAAVPLPSDPPVDMAAHGA
                     GSSSVEPATSGLLRAFPASNAPAGNPTTPERVALGRLLFFDPVLSNDDDIACATCHHP
                     DLGFSDGRATSVMPGGDAPARNALTLWNVVYDQSLFWDGRVGSLEAQALVPQTHADEM
                     AVA"
     gene            complement(639..1040)
                     /locus_tag="PK829_14850"
     CDS             complement(639..1040)
                     /locus_tag="PK829_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOU42544.1"
                     /translation="MIRRRRPIDQVLTSLILLAVVCLAGCGGAAAPPPTPTLPPEMAA
                     GQRVFVAHCGACHSTSPETVIVGPSLAGIAARGGERIDGLDARAYVYSSILQPGGFLV
                     PGYEDLMPHDLAKKLTGEEMDYVVAYVLALR"
     gene            1129..>1592
                     /locus_tag="PK829_14855"
     CDS             1129..>1592
                     /locus_tag="PK829_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU42545.1"
                     /translation="MTDPAQLPPTWTTRRVEVRDATAGDTPVLTALFNACAYVEPWDP
                     TFRPVEQAEIDKLVRRSLADDEHANFRLQALRLRSGGQPFGYFHLFHGAPQPDVCWIS
                     LFVIHPDFQGQQLGQEVVNGLAYHLREAGYWAIWLEVYLRNWPALRFWTNAG"
BASE COUNT          257 a          582 c          524 g          229 t
ORIGIN      
        1 cggccacggc catctcgtcg gcgtgggtct ggggcacgag ggcctgcgcc tccagcgagc
       61 cgacgcgacc gtcccagaag aggctctggt catagaccac gttccagagg gtcagggcgt
      121 tgcgggcggg cgcgtcgccg ccgggcatga ccgacgtggc ccggccgtcg ctgaagccga
      181 ggtcggggtg gtggcaggtg gcgcaagcga tgtcgtcgtc gttcgacagc acggggtcga
      241 agaacagcag ccggcccaac gccacccgtt ccggcgtcgt cgggttgccg gccggcgcgt
      301 tgctggccgg gaaggcgcgc agcaggcccg acgtggccgg ctcgacgctg ctggagccag
      361 ccccgtgggc ggccatgtcc accggcgggt cgctgggcaa gggcacggcc gcggccacga
      421 ccagcgccag cagcaccacg cccaccaccg cgacgacaat ccacaacaac actttcccca
      481 atcgtttcat gctctcctcc actgaccgcc aaccaccgac cgccaaccgc caaccgccga
      541 cgaccggcca cagaccacta ccctaacctc ctatctcccc cgctcccctg ctcccctgct
      601 cccctgctcc cccacccggc ggacacgccg gtctgctcct accgcagcgc caagacatac
      661 gcgacgacgt aatccatttc ctccccggtc agtttcttgg ccaggtcgtg gggcatcagg
      721 tcctcatagc cgggcacgag gaagccgccc ggctggagga tggagctata gacgtaggcg
      781 cgggcgtcca ggccgtcaat gcgctcgccg ccgcgggcgg caatgccggc cagcgacggg
      841 ccgacgatga ccgtctccgg actagtgctg tggcacgccc cgcagtgggc cacgaagacg
      901 cgctgcccgg cggccatctc cggcggcaac gtcggcgtgg gcggtggcgc ggccgccccg
      961 ccgcagcccg ccaagcagac tacggccagc aggatgaggc tggtaagaac ttggtcgatc
     1021 gggcggcggc ggcgtatcat agtggctgtt ctctgagggt cggcgaattg tacggaggcg
     1081 gatggacgcg ggcaacactg tgaaaagcat cacaaggccg gccggggcat gactgacccg
     1141 gcccaactgc ccccaacctg gacgactcgc cgcgtggagg tgcgcgacgc cacggccggc
     1201 gacaccccag tgctgacggc cctcttcaac gcctgtgcct acgtcgagcc gtgggacccc
     1261 acctttcgcc ctgtcgagca ggcggagatc gacaagctgg tgcgccgcag tctggccgac
     1321 gacgagcacg ccaacttccg cctgcaagcc ctgcgcctgc ggtcgggcgg ccagccgttc
     1381 ggctacttcc acctcttcca cggcgcgccg cagccagacg tgtgctggat ctcgctgttc
     1441 gtcatccacc ccgacttcca ggggcagcaa ttaggccagg aagtggtcaa cggcctggct
     1501 tatcacctgc gcgaagccgg ctattgggcc atctggttgg aggtctacct gcgcaactgg
     1561 ccggccctgc gcttctggac gaacgccggc tt
//
LOCUS       DAOSMQ010000625         1587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_78176_length_1587_cov_4.611619, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000625 DAOSMQ010000000
VERSION     DAOSMQ010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78176_length_1587_cov_4.611619"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PK829_14860"
     CDS             <1..803
                     /locus_tag="PK829_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU42546.1"
                     /translation="DRGEIEAALAGKLPALVALDDIRCDLHMHTEWSDGTLSVLDMAR
                     AARDGGLRMVAITDHSVSLGIANGLSVERLRAQAAEVRAANEALGPDFRVLHGTEMEI
                     RADGTLDYPDDVLAELDFVIASLHTGLGQPRDQAMTRILNAIHNPHVDMIAHPTGRLL
                     PDRAGADLDMDAVFAAAAQTGTILEVNAHPARLDLRDTHVRRALAMGVRIAINTDAHR
                     PEEFGNRSFGVATAQRGWATAADVVNTWPPEELLAFIRAKNEGGSRPA"
     gene            829..>1587
                     /locus_tag="PK829_14865"
     CDS             829..>1587
                     /locus_tag="PK829_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU42547.1"
                     /translation="MSDVADPAHMELDAQMLASLRRVAAAHTYPAGTILTHQGQTEHT
                     FYVIEDGGVVVLRRMEDGGEQMLNTLGPRQSFGEMALLDDNPRLATIRTLSETRVLEI
                     TADSFRELLRHDPDLAMHITRRVLANLRTLDQLAIHDLRAKNALLQKAYLELQAAQAE
                     LVEKERLERELELAAEMQRSLLPAVLPRYDDYRFAAYLAPARHVGGDLFGVQPLDEEY
                     VALLIADVADKGMYAALLMAVTRTLFMQEAQRSRS"
BASE COUNT          282 a          536 c          517 g          252 t
ORIGIN      
        1 aagaccgggg cgagattgaa gccgcgctgg ccggcaaatt gccggcgctg gtggcgctcg
       61 acgatattcg ttgcgacctg cacatgcaca cggaatggtc cgacggcacg ttgagcgtgc
      121 tggacatggc ccgcgcggcg cgtgatggcg gtctgcgcat ggtggccatc accgaccatt
      181 cggtcagcct gggcattgcc aacggcctgt cggtggagcg cttgcgggcg caagcagccg
      241 aagtgcgcgc cgccaacgaa gccctggggc cggacttccg tgtgttgcat ggcacggaga
      301 tggagatccg cgccgacggc acactcgact atcccgacga cgtgctggcc gaactggatt
      361 tcgtcattgc cagcctgcac accggcctgg gccagccgcg tgaccaggcc atgacgcgca
      421 ttctgaatgc catccacaac ccacacgtgg acatgattgc ccaccccacc ggccgcctgt
      481 tgcccgaccg ggccggagcc gacctggata tggatgccgt ctttgccgcc gcggcgcaga
      541 ccggaacgat tctcgaagtc aacgcccacc cggcgcgcct cgacctgcgc gacacccacg
      601 tgcgccgtgc gctggcaatg ggcgtccgca ttgccatcaa caccgacgcc caccggccgg
      661 aggagtttgg caatcgcagc tttggcgtgg ccacggccca acgcggctgg gccacggccg
      721 ccgacgtggt caacacctgg ccgcccgaag aattattggc cttcatccgt gccaagaacg
      781 agggcggaag ccggcccgcc tagccgatgg gcatcttgag tgttaccgat gtcagacgta
      841 gccgaccccg cccacatgga acttgacgcc cagatgctgg ccagcttgcg gcgcgtggcc
      901 gcggcccaca cctatccggc cggcaccatc ttgacccacc agggccagac ggagcatacc
      961 ttctatgtca tcgaagacgg tggcgtagtt gttctgcggc ggatggagga cggcggcgag
     1021 caaatgctca acaccctggg gccgcgccag tcattcggcg agatggccct cctggacgac
     1081 aacccccgcc tggcaacgat acggacgctc agcgagacgc gcgttttgga gatcaccgcc
     1141 gacagcttcc gggagttgct gcgccacgac cctgacctgg cgatgcacat cacccggcgc
     1201 gtgctggcca acctgcgcac gctcgaccag ttagccatcc atgacctgcg ggccaagaac
     1261 gccttgttgc agaaagccta tctggagctg caagcggccc aggcggaact ggtggagaag
     1321 gagcgactgg agcgggaact ggagttggcc gcggagatgc agcgcagcct cttgccggcc
     1381 gtcctgccgc gctatgacga ctaccgtttc gccgcctacc tggctccggc gcgccacgtc
     1441 ggcggcgacc tgttcggcgt gcagccgctg gatgaagagt acgtggccct gctgatcgcc
     1501 gacgtggccg acaaaggcat gtacgcggct ctactgatgg ccgtcacgcg gacgttgttc
     1561 atgcaggagg cgcagcgttc gcgctcg
//
LOCUS       DAOSMQ010000626         1586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_78244_length_1586_cov_4.936643, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000626 DAOSMQ010000000
VERSION     DAOSMQ010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78244_length_1586_cov_4.936643"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PK829_14870"
     CDS             complement(<1..349)
                     /locus_tag="PK829_14870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42548.1"
                     /translation="MRLLVCAGGTGGGIYPALAAVAELRQQGLAATDILWIGAKGEME
                     ETLVPRAGLQLATIPAGPLVGVPLGTRLRSAVKMARGFGAALGHIGRFRPAVLFITGG
                     YVAAPVALAARARR"
     gene            complement(351..1529)
                     /locus_tag="PK829_14875"
     CDS             complement(351..1529)
                     /locus_tag="PK829_14875"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOU42549.1"
                     /translation="MAPINVVKRRGEPLLAGRNKYNVDVWLLLGTAALLVFGFLMVYS
                     TTFDLGFRFKDNPVYYMTRQLGAMALGTAIIAIMMQFDYHALRILSVPIIVVTIVLLV
                     FLLFFGQVNYGAARALSQGSYQPSELAKLATILYIAHWLHSKGDRIKQVNYGLLPFSI
                     ITGVMFALIVLQPALSTAILVGLISFTLFFVAGADLKQVLVVGLVAGGVVLLMMTILP
                     HAAQRVSDFRAALADPFKASYQVRQALAALAQGGLFGVGLGQGVQKFGPLPLAHTDGV
                     FAIVGEELGLVGALGLIALFCFLAYRGFRVATRARDTYGFLLAVGVTVWIAYQALINL
                     AVITSTIPFTGMPMPFLSYGGSSMLITLIGVGILLNVSRDGKAAAALGGRQPRRQQPE
                     "
BASE COUNT          315 a          470 c          569 g          232 t
ORIGIN      
        1 cgcggcgggc gcgggcggcc agggcgacgg gcgcggcgac gtagccgccg gtgatgaaca
       61 gaaccgccgg tcggaagcgg ccgatatggc ccagggccgc gccgaagccg cgcgccatct
      121 tgacggcgct acgcagccgc gtgcccagcg gcacgccgac caacggcccg gcggggatgg
      181 tcgccagttg taggcccgcg cgtggtacca gtgtctcttc catctcaccc tttgcgccga
      241 tccacagaat atcggtagcc gccaggccct gctgtcgcaa ctcagcgacc gcggccaggg
      301 ccggatagat gccaccaccc gttccgccgg cgcacaccag tagtctcatg tcattccggt
      361 tgctgacggc gcggctggcg gccgccgagg gccgcggcgg ctttgccatc gcgcgagacg
      421 ttcagtaaga tgccgacgcc gatcagcgtg atcagcatcg atgagccgcc ataactgaga
      481 aagggcattg gcatccccgt gaaggggatg gtcgatgtga tgacggccag gttgatcaaa
      541 gcctggtagg cgatccagac ggtgacgccg accgccagca gaaaaccata ggtgtcgcgg
      601 gcgcgcgtgg ccacccggaa accgcgatag gctaaaaagc agaacagggc gatcaggccc
      661 agagcgccga caaggcccag ttcttcgccg acgatggcga agacgccgtc ggtgtgggcc
      721 aggggcaagg ggccgaactt ctggacgccc tggccgaggc cgacgccgaa cagcccgccc
      781 tgggccaggg cggccagggc ctgacgcacc tggtagctgg ctttgaaggg atcggcgagg
      841 gcggcgcgga agtcgctgac gcgttgggcg gcgtggggca ggatggtcat catgagcagg
      901 acgacgccgc cggcgaccag gccgacgacc agcacctgct tcaggtcggc cccggcgacg
      961 aagaacaggg tgaagctgat caggccgacg aggatggcgg tgctgagggc gggctgcaag
     1021 acgatcaggg cgaacatgac gccggtgatg atcgagaagg gcaggaggcc gtagttgacc
     1081 tgcttgatac ggtcgccttt ggagtggagc cagtgggcga tgtagaggat ggtggccagc
     1141 ttggccagct ccgacggctg ataggaacct tgcgacagcg cccgcgccgc cccgtagttg
     1201 acctggccga agaagagaag gaagaccagc aagacgatgg tcacgacgat gatcggtaca
     1261 gacaggatgc gcagggcgtg gtagtcgaac tgcatcatga tggcgatgat ggcggtgccc
     1321 agggccatcg cgccgagctg gcgggtcata tagtagaccg ggttgtcctt gaaacgaaag
     1381 ccgaggtcga aggtggtgct atagaccatc aggaagccga agacgagcag ggcggccgtg
     1441 cccagcagga gccagacgtc aacgttgtat ttgttgcggc cggccaacag aggttcgccg
     1501 cgtctcttga ccacgttaat cggtgccatc atctctcccg tggtggggcg atttgccaaa
     1561 tcgccctaca gttctcctac cagtag
//
LOCUS       DAOSMQ010000627         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_78593_length_1581_cov_4.119921, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000627 DAOSMQ010000000
VERSION     DAOSMQ010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78593_length_1581_cov_4.119921"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PK829_14880"
     CDS             complement(<1..997)
                     /locus_tag="PK829_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU42550.1"
                     /translation="MTVGTMTTNAKRILIVDDEAPLRFLLSKQLTRAGFEVVAAADGQ
                     AALAAVDESIAAVVLDVVMPGMDGFEVCRRLKADPRVAHIPVIFLSASFSGDFRRRAF
                     RLGGADFLAKPFQTEELPAYLHALLGDATRHQPAAGGRVVAVVGSDRAAGSAAAVRMA
                     ETDALRGDAPIMLIDLELPAGAIGARLQLAGGPNVRVLLHDTGEAITYEEIGRVAQRV
                     HFGLEVIPAPFTPSPLGGDEPDARRLSDMLDLLAAAGYGVVVHLGATPEALAAAALRR
                     SQAVYTVGPVDGEQRKALVALGAAQEAITPLAETGSGRPARPAETARPAPRPAVRA"
     gene            1116..>1581
                     /locus_tag="PK829_14885"
     CDS             1116..>1581
                     /locus_tag="PK829_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOU42551.1"
                     /translation="MSRSIEFDIDAGGIAVLRVNRPAARNALTWSAQEGFAQAVAAAA
                     RSTTARALIVTGTGEAFVSGGDLKELAGHPEASAGERLNRVMSAALDGLIALPFPVIA
                     AVNGDAIGGGCEILTACDLRLAAPQARFAFRQVHNGLTTGWGGGGRLVELIGQ"
BASE COUNT          222 a          560 c          569 g          230 t
ORIGIN      
        1 gcgcgcggac ggccggccgc ggggcaggcc gcgcggtttc cgcgggccgc gccgggcggc
       61 cgctgccggt ttcggccagg ggcgtgatgg cctcttgggc cgcgcccagg gcgaccaacg
      121 ccttgcgctg ctcgccatcg actggcccca cggtgtagac agcctgcgac cggcgcagag
      181 cggccgcggc cagcgcttct ggcgtggccc ccaggtggac aaccacccca tagccggctg
      241 cggccagtag gtcgagcatg tcgctcagcc gccgcgcgtc cggctcgtcg ccgcccagcg
      301 gcgacggcgt aaagggcgcg gggatgacct ccaggccaaa gtgtacccgc tgggccacac
      361 gcccgatttc ctcgtaggtg atggcttcgc cggtgtcgtg gagcaagacg cgcacgttgg
      421 ggccgccggc cagctgtagc cgcgcgccga tcgcgcccgc gggcagttcc aggtcgatga
      481 gcatgatcgg cgcgtcgccg cgcagggcgt ctgtctcagc catccgcacg gcggcggccg
      541 agccggcggc ccggtcgctg cccacgacgg ccaccacgcg cccgccggcg gccggctggt
      601 ggcgggtggc gtcgccgagc agagcgtgga gataggcggg tagttcttcg gtctggaaag
      661 gcttggccag aaagtcggcc ccgcccaggc ggaaagcccg ccggcggaag tcaccgctga
      721 acgatgccga caggaagata accgggatgt gggccacgcg tgggtcagcc ttcaggcggc
      781 ggcagacctc gaagccgtcc atgcccggca taaccacgtc tagcaccacg gcggcgatgc
      841 tctcgtccac ggcggccagc gcggcctggc cgtcggcggc ggccactacc tcgaacccgg
      901 cgcgggtcag ttgcttgctc aacaggaagc gcagcggcgc ttcgtcgtct acgatcagga
      961 tgcgtttggc gtttgtcgtc atcgttccca cagtcattgg tcgtaggcag ccgcccacgg
     1021 aagtcgtctg tccaggatca tagaggggcc gggttacgag cgaccttaag ggccgggcaa
     1081 gtgcgctgag aggcgttttc tgctatcgtt agcccatgag caggagcatc gagttcgaca
     1141 tcgacgctgg cggcattgcc gtgctgcgcg tcaaccggcc cgcggcgcgc aacgcgttga
     1201 cgtggagcgc ccaggagggt ttcgcccagg ccgtggccgc ggcggctcgc tcgaccactg
     1261 cccgcgccct gatcgtcacc ggcacgggcg aggcctttgt gtccggcggc gacctgaagg
     1321 aactggccgg ccaccccgaa gcgtccgccg gcgagcgcct caaccgcgtc atgagcgccg
     1381 ccctcgacgg cctcatcgcc ctgcccttcc cggtcatcgc cgcggttaac ggcgacgcca
     1441 tcggcggcgg ctgcgaaatc ctgaccgcct gcgatctgcg cctggccgcg ccgcaggccc
     1501 gcttcgcctt ccgtcaggtt cacaatggcc tgacgaccgg ctggggcggc ggcggccggc
     1561 tggtcgaact catcggccag a
//
LOCUS       DAOSMQ010000628         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_79155_length_1573_cov_3.548090, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000628 DAOSMQ010000000
VERSION     DAOSMQ010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79155_length_1573_cov_3.548090"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK829_14890"
     CDS             <1..467
                     /locus_tag="PK829_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOU42552.1"
                     /translation="DTSPSGFLGGMPLAMAVGLGDQTLLRATGLRPWPTDKVLLVDGR
                     DLDPGERDNLAAAAIAHAPDIETLLQPDALPAGPLYVHFDVDIIDPAEMQAVHYPAPG
                     GPAAQLVADLLRALAATDRVVAVSVSTWDPALDPDGHAQAVAQAALNALFGR"
     gene            620..>1573
                     /locus_tag="PK829_14895"
     CDS             620..>1573
                     /locus_tag="PK829_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HOU42553.1"
                     /translation="MSTHIVGPRSVSKPPSAELSAAYQAGVRAYAVDYYVPDYIPQDT
                     DLLCAFRIRPRGVDMVEAAAAVAAESSTGTWTEVWSNQLTDIDFYKAKVYAIVDDIAY
                     IAYPLDLFEENSVVNIMSSIVGNVFGFKAVGALRLEDMRIPLALVKTFPGPRVGIYDE
                     RVWANKWGRPLLGGTVKPKLGLSPKAYSTIIYECLVGGLDTSKDDENMNSQPFSRWRD
                     RFMYAQDAVERAMAETGEFKGHWHNCTAGSTEESLRRLEYAAELGSRMVMYDFLTAGF
                     AASADVFKRAAELDVIVHCHRAMHAVFTRQPDHGIAMRVVAK"
BASE COUNT          263 a          572 c          457 g          281 t
ORIGIN      
        1 cggacacatc gcccagcggc ttcctgggcg gcatgcccct ggcgatggct gttggcctgg
       61 gcgaccagac gctgttgcgc gccaccggcc tgcgcccctg gcccacggac aaagtcctgc
      121 tggtcgacgg ccgcgacctg gaccccggcg agcgcgacaa cctcgccgcg gcggccatcg
      181 cccacgcgcc ggacatagag acattgctgc aacccgacgc cctgcccgcc ggcccgctct
      241 acgttcactt cgacgtcgac atcatcgacc cggccgaaat gcaggccgtt cactaccccg
      301 cccccggcgg cccggcggcc caactcgtgg ccgatctctt gcgcgccctg gcggccaccg
      361 accgcgttgt ggccgtttcc gtctccacct gggacccggc gctcgacccc gacggccacg
      421 cccaggcagt tgcccaggcg gccctcaacg ctcttttcgg ccgctaacca ctgcccaaga
      481 ccccacattc tacttattga cgcgctgcgt catcgggtgc tatactgctt tgtgaaatcc
      541 ctcacaatga tggattcatt gtccctcggt tgtctgtcat gtctgtatcg gtcgcccgac
      601 cgaactagga ggtcaatctg tgagcaccca tattgttggt ccccgaagcg tatcgaagcc
      661 gccgtcggcc gaattgagcg cggcctatca ggccggcgtg cgcgcctatg ccgtcgatta
      721 ctacgtgccc gattacatcc ctcaggacac cgacctgctg tgtgccttcc gcatccggcc
      781 gcgcggcgtt gatatggtcg aggccgctgc cgccgtcgcc gcggagtcct ccaccgggac
      841 gtggactgag gtctggtcta accaactcac cgatattgac ttctacaaag ccaaggtcta
      901 cgccatcgtc gacgacatcg cctacatcgc ctaccccctt gacctgtttg aggagaacag
      961 cgtcgtcaac atcatgtcct ccatcgttgg caacgtcttc ggcttcaagg ccgtcggcgc
     1021 gctgcggctg gaagatatgc gcatcccgct ggctctggtg aaaaccttcc ccgggccgcg
     1081 cgtgggcatc tatgacgagc gcgtctgggc caataagtgg ggccggccgc tgctgggtgg
     1141 cacggtcaag ccgaagctgg gcctgtcgcc caaggcatac tcgaccatca tctacgagtg
     1201 tctcgtcggc ggcctggaca cctccaaaga cgacgagaat atgaacagcc agccgttcag
     1261 ccgctggcgc gaccgcttca tgtatgccca ggacgccgtc gagcgggcca tggccgagac
     1321 gggcgagttc aagggccact ggcacaactg caccgccggc agcactgagg aaagcctgcg
     1381 ccgcctggaa tacgccgccg agttgggcag ccgcatggtc atgtatgact tcctgaccgc
     1441 cggcttcgcc gcctcagccg acgtcttcaa gcgcgccgcc gagctggacg tcatcgtcca
     1501 ctgccaccgg gccatgcacg ccgtcttcac ccgccagccc gaccacggca tcgccatgcg
     1561 cgtcgtggcc aag
//
LOCUS       DAOSMQ010000629         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_79639_length_1566_cov_3.525480, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000629 DAOSMQ010000000
VERSION     DAOSMQ010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79639_length_1566_cov_3.525480"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK829_14900"
     CDS             complement(<1..413)
                     /locus_tag="PK829_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOU42554.1"
                     /translation="MRLDKLTEKSREAVLEAQHLAESYNHPAVEPEHLLLTLLDQEGG
                     VVPSVVRKIGGDVALLRGGVEAALAKLSRATGSSVQVGLSRELTAILNEAEAIAGAMK
                     DDFTSTEHILMAMAQPKAGRVRELLLATGDTVNEG"
     gene            594..>1566
                     /locus_tag="PK829_14905"
     CDS             594..>1566
                     /locus_tag="PK829_14905"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOU42555.1"
                     /translation="MAPDHGTWRAADTLARSGKGGPGGGVVDGESVLLWAEFSDGQRP
                     PALPSVLLPHLVVACSCSSNQFPCRHVLALLLLDWAAPTTAAGAPEWAATRLETGLRA
                     ARAGATPAGDETRPAALAAGLDELARRLRDEVGHGLAGLAAGDRRPWLAAADRLNDAY
                     APAAARELRALADLPGATPNWPEQLLPRLGRLMLLAEAFGRLDALSPGERGDALAATG
                     WPPRPGDDHVADTWLALGRRPLGDGDQRGLRTWLRGLSSGRWALLEEFRPARRPEGRC
                     FPAGARLAGELAFLPSAWPLAAQPVEALRLLAEAGDSDPAGQSIAAAV"
BASE COUNT          198 a          552 c          568 g          248 t
ORIGIN      
        1 tcaccctcgt tcacggtgtc accggtggcc agcagcagtt cgcgcacgcg cccggctttg
       61 ggctgggcca tggccatgag gatgtgctcg gtggaggtga agtcgtcctt catcgccccg
      121 gcgatggctt cggcctcgtt gaggatggcg gtcagttcgc ggctcaggcc cacctgcacg
      181 gacgagccgg tggcgcgcga cagcttggcc agggccgcct caacgccgcc acgcagcaaa
      241 gcgacgtcgc cgccgatctt gcggacgacc gacggcacga cacccccctc ctgatccagc
      301 agcgttagca acaggtgttc cggctcgacg gccgggtggt tatagctctc ggccagatgc
      361 tgggcttcca gcacggcctc gcgactcttt tcggttagtt tatccagacg catgggctga
      421 ttcctcgcac aatactttaa ggctaagtat ccgccagaga ggtcagaatt acgttaatcg
      481 cggatcagaa tggcgtaaga tgcaaccgcg ccggccggaa gtgcgtaata atgaacagta
      541 tgccggttgt gtggacaggg cggcggtcgt tgtcgctgcc gatgattgcg ggaatggccc
      601 ccgaccacgg gacgtggcgg gcggccgata cattggcgcg cagcggcaag ggcggccccg
      661 gcggcggcgt cgtagacggc gagtccgttc ttctctgggc cgaattcagt gacggccaac
      721 gcccgccggc ccttccctcc gtcctgctgc cccatctggt ggtcgcctgc tcctgttcct
      781 cgaaccaatt tccctgccgc catgtgctgg ccctgctgct gctcgattgg gccgctccga
      841 caaccgcggc cggcgctcct gagtgggcgg ccacgcggct ggaaaccggg ctgcgggcgg
      901 cgcgggcggg cgcgacgcca gccggcgacg agacgcggcc ggccgccctg gccgccgggc
      961 tggacgagct ggcccgccgg ctgcgtgacg aagtgggcca cggcctggcc gggctggccg
     1021 cgggtgatcg tcgcccctgg ctggccgcgg ccgaccggct caatgacgcc tatgccccgg
     1081 ccgcggcccg tgagttgcgc gccctggccg acctgcccgg cgcgacgccc aattggccgg
     1141 agcaactgct gccccgcctc ggccggctga tgctgctggc ggaggcgttt ggccgccttg
     1201 acgcgctgtc gcccggcgag cggggcgacg ccctggctgc caccggctgg ccgccccgcc
     1261 ccggcgacga ccatgtggct gatacctggc tggccctcgg ccggcgaccg ctgggcgacg
     1321 gcgaccagcg ggggctgcgc acgtggctgc gcgggctgtc gtccggtcgc tgggcgctgc
     1381 tggaagagtt ccggccggcc cggcggccgg aggggcgctg tttccccgcc ggggcgcggc
     1441 tggcgggcga attggccttc ctgcccagcg cctggccgct cgccgcccaa ccggtcgagg
     1501 cgctgcgcct actggccgag gccggggaca gcgatcccgc cggccagagc atcgccgcgg
     1561 ccgtgg
//
LOCUS       DAOSMQ010000630         1562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_79925_length_1562_cov_3.110153, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000630 DAOSMQ010000000
VERSION     DAOSMQ010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79925_length_1562_cov_3.110153"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..780
                     /locus_tag="PK829_14910"
     CDS             100..780
                     /locus_tag="PK829_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42556.1"
                     /translation="MATSMGLVRWLLVPLLAGLLSPPAQDGNRAGIVVDFGEETTTAC
                     VAFAEPDISSYEALVRSGLALEVNQQGDGAAVCRIEATGCAADDCFCACKGQDCVYWS
                     YWQRVDEAWQYAAIGASQHRVSDGDVDGWVWGPGTVAEAPPPPEVNFDDICGGAGDEP
                     GTEATAVAMLGAPATEGLSTAATVETPATAPTAGPSVWTYGGFVVLLLLLGGLLWLAR
                     RRAGGGGA"
     gene            783..>1562
                     /locus_tag="PK829_14915"
     CDS             783..>1562
                     /locus_tag="PK829_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOU42557.1"
                     /translation="MARRLATAALFLLALAIGAVAFLYPFLLPAAPAPAPGAATSGTV
                     AVPLLSAVLVTLCLAVLLVELQGQAAGAKTVAMLGVLVAATSTLRFIEVAIPGPGGFS
                     PIFAPIILGAYVFGPRFGFLLGAMTMLVSGIITAGVGPWLPYQMFTAGWVGITAGWLP
                     HLGRPRAEVAMMALLGLLWGFAYGAIMSFYTWPFLVGNAQTTWIPGAGAADALRRYAA
                     FYLTTSLLWDAVGAVGNVILLAALGAPTLRALGRFRDRLLFE"
BASE COUNT          176 a          510 c          594 g          282 t
ORIGIN      
        1 tgggcagtgg cccggtcgat gactactaca cgaccgtgct ggccattccc gccgctgccg
       61 gccgggcctg gccgctggcc gccggcgcgg agtaggcgca tggccacgtc gatgggtctt
      121 gtccgctggc tgctcgtgcc gctcctggcc ggcctcctct cgccgccggc gcaggacggt
      181 aaccgcgccg gtatcgtcgt tgacttcggc gaggagacga cgacggcctg tgtggccttt
      241 gcggagccgg acatcagcag ctacgaggcg ctggtgcgtt cggggctggc gttggaggtc
      301 aaccagcagg gcgacggcgc ggccgtctgc cggatcgaag ccactggctg cgccgccgat
      361 gactgctttt gcgcctgcaa gggtcaggat tgcgtctatt ggtcttactg gcagcgggtg
      421 gacgaggcat ggcaatatgc cgccatcggc gccagccagc accgggtgag cgacggcgac
      481 gtagatggct gggtgtgggg gccggggacg gtcgctgaag cgccgccgcc gccggaggtg
      541 aacttcgacg atatttgtgg tggcgcgggc gatgagccag ggacggaagc gacggccgtg
      601 gccatgctcg gcgcgccggc gacagaggga ctgtctacag ccgcgacggt cgagacgccg
      661 gcgacagccc cgacggccgg gccaagcgtg tggacctatg gcggcttcgt tgtcctgttg
      721 ctgctgctcg gcgggctgct gtggctggcg agacggcggg cgggtggggg cggggcataa
      781 ccgtggcccg acgccttgcc accgcggcgc tctttctgct ggcgctggcc atcggcgcgg
      841 tggcctttct gtatccgttc ctgttgccgg ccgcgcccgc ccctgcgccg ggcgcggcga
      901 cgtcggggac ggtggccgtg ccgttgctgt cggccgtgct ggtgacgctg tgcctggccg
      961 tcctgctggt cgagctccag gggcaggccg cgggcgcgaa gacagtggcc atgctgggtg
     1021 tcctggtggc ggcgacatcg acgctgcgtt tcatcgaggt agccatcccc gggccgggcg
     1081 gcttctcgcc cattttcgcg cccatcatcc tcggcgcata cgtcttcggg ccgcgcttcg
     1141 gcttcttgct aggagcgatg acaatgctcg tttccggcat catcaccgcc ggcgtggggc
     1201 cgtggctgcc ctaccagatg ttcactgccg gctgggtggg catcactgcc ggctggctac
     1261 cccacctggg ccggccgcgg gcggaggtgg ccatgatggc gctgctgggc ctgctgtggg
     1321 gctttgccta cggggccatt atgagcttct acacctggcc cttcctggtg ggcaacgccc
     1381 aaacgacctg gataccgggc gcgggcgcgg ccgacgcgct gcggcgctac gccgccttct
     1441 acctgacgac ctcgctgctg tgggatgcgg tcggcgcggt gggcaacgtc attctgctgg
     1501 ccgcgctggg cgcgccgacg ctgcgcgccc tgggccgctt ccgcgaccgg ctgttgttcg
     1561 ag
//
LOCUS       DAOSMQ010000631         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_81425_length_1542_cov_4.395427, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000631 DAOSMQ010000000
VERSION     DAOSMQ010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81425_length_1542_cov_4.395427"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1173
                     /locus_tag="PK829_14920"
     CDS             25..1173
                     /locus_tag="PK829_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOU42558.1"
                     /translation="MSIDTFTITDNHRLNLPSIDMPPRILLGAGPSNSAPRVLAAMLA
                     PTVGHLDPTFIQVMGEVSELLRYAWQTDNEYTIAISGTGSSGMEAAFANVVEPGDRVL
                     VGVNGYFGRRMVDMAGRYGADVRCLETTWGAAFSPEELRAGMETHRPRVLALVHAETS
                     TGVRQPLDGLAELCREHDCLLLVDTVTSLGGVPLYADAWGLDVAYSGSQKCLSSPSGL
                     APITFGPRALARIANRKTPIANWYMNSELLGKYWFGSPRAYHHTAPMHLFYALREGLR
                     LVAEEGLAARWARHQAVAERLWAGLGEMGLEPHVPLEIRLPSLTTVRVPAGVDAAAVS
                     RRLLTEYNIEIAGGLGDLAGKVWRVGLMGYNARPESVAVLLRALADVL"
     gene            1183..1542
                     /locus_tag="PK829_14925"
     CDS             1183..1542
                     /locus_tag="PK829_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005302071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOU42559.1"
                     /translation="MDLEELRATLLARRGATEELPFGPEVLVYKVAGKMFALVAWQER
                     PLTVSLKCEPGQALVWRAVFPSVRPGYHLNKTHWNTLTLDGRIPPEDVREMIDHSYER
                     VVAGLTRKQRAGLAEEA"
BASE COUNT          226 a          530 c          542 g          244 t
ORIGIN      
        1 ttcccgccgc ggagcgaggg aaccatgagt atagacacct tcaccatcac tgacaatcat
       61 cgcctcaacc tgccgtccat tgacatgccg ccgcgcatcc tcctgggcgc ggggccgtcg
      121 aacagcgccc cgcgcgtgct ggcggccatg cttgccccga ccgtcggcca ccttgacccg
      181 acctttattc aggtcatggg cgaggtcagc gagctgttgc gctacgcctg gcagacggac
      241 aacgagtaca ccattgccat cagcggcacc ggcagctcgg gcatggaggc cgccttcgcc
      301 aacgtcgtcg agccgggcga ccgggtgctt gtcggcgtca atggctactt cggccggcgc
      361 atggtcgata tggccgggcg ctatggggcc gacgtgcgct gcctggagac aacgtggggc
      421 gcggccttct cgcccgagga gctgcgcgcg ggcatggaaa cccaccggcc gcgcgtgctg
      481 gccctcgtcc acgctgagac ctccaccggc gtgcgccaac cgctggatgg gctggccgaa
      541 ctgtgccgcg agcacgactg cctgctgctg gtcgatacgg tcaccagcct gggcggcgtg
      601 ccgctctacg ccgacgcctg gggcctcgac gtggcctata gcggctcgca gaagtgcctc
      661 agcagcccgt cgggcctcgc gccgatcacc ttcggcccgc gggcgctggc gcgcatcgcc
      721 aaccgcaaga cgcccatcgc caactggtac atgaacagcg agttgctggg caagtactgg
      781 ttcgggtcgc cgcgcgccta tcaccacacc gcgccgatgc acctcttcta cgccctgcgc
      841 gaagggctgc ggctggtggc cgaagagggg ctggccgccc gctgggcgcg tcatcaggcc
      901 gtggccgagc gcctttgggc cgggctgggc gagatggggc tggagccgca cgtgccgttg
      961 gagattcgcc tgcccagcct gacgacggtg cgcgtgcccg ctggcgtcga tgccgccgcc
     1021 gtctcgcgcc ggttgctgac ggagtacaac atcgagatcg ccggcggcct gggcgatctg
     1081 gccggcaagg tctggcgcgt cggcctgatg ggctacaacg cccggccgga gtcggtcgcc
     1141 gtgctcttgc gggcgctggc cgacgtgctc taggggacgg gcatggacct ggaggagctg
     1201 agagccactc tcttggcccg gcggggggcg acggaggaat tgcccttcgg ccccgaagtg
     1261 ctggtctaca aggtggccgg caagatgttc gcccttgtcg cctggcagga acggccgttg
     1321 acggtcagcc tgaagtgcga gccgggccag gcgctggtct ggcgggccgt ctttccgtcc
     1381 gtccgccccg gctatcacct caacaagacc cactggaaca cgctgaccct cgacggccgc
     1441 attcccccgg aagacgtgcg cgagatgatc gaccactctt acgagcgggt cgtggccggg
     1501 ctgacgcgga agcagcgggc cgggttggcc gaggaagcct ag
//
LOCUS       DAOSMQ010000632         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_81564_length_1540_cov_4.542761, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000632 DAOSMQ010000000
VERSION     DAOSMQ010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81564_length_1540_cov_4.542761"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="PK829_14930"
     CDS             <1..119
                     /locus_tag="PK829_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOU42560.1"
                     /translation="FVTKLLGEVTIVGPGAGRVETGYAILSDLLDIHQRSTL"
     gene            215..517
                     /locus_tag="PK829_14935"
     CDS             215..517
                     /locus_tag="PK829_14935"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU42561.1"
                     /translation="MTDSLDFAKAMADETRQRIMALLCCRWLCVGDIVTEMQAISQPT
                     VSHHLAILREAGLVHVRRDGKQVFYSLNQDEVAVCCGRIMRNFAPEVPVAGIAVSE"
     gene            577..1365
                     /gene="arsM"
                     /locus_tag="PK829_14940"
     CDS             577..1365
                     /gene="arsM"
                     /locus_tag="PK829_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOU42562.1"
                     /translation="MTDDNTIREAVQSHYAAVASGGSCCGDSCCTPGGDAAVDVSLDS
                     LYGNLYTADTTWLPPAVTGLSLGCGDPITLAGLQPGQTVLDLGSGGGIDCFMAARQVG
                     PDGHVIGVDMTPEMLAQAERNKSTVGLENVEFRQGVIEALPVADGTVDVILSNCVINL
                     SPDKPAVFREAYRVLRPGGRLAVSDMMTVGLFSPAERADLSAWAGCVSGAEDVAHYVA
                     AMRAAGFEDISIVDKAAPGEELAGQLPETGPARLFSARVTAVKH"
BASE COUNT          265 a          494 c          506 g          275 t
ORIGIN      
        1 cgttcgtgac caagctgctg ggcgaagtga ccatcgtcgg ccccggcgcg gggcgcgttg
       61 agaccggcta cgccattctg tccgacttgc tagacattca ccagcgcagc acgctctagc
      121 agcgccggcg gctgtccaga gccagcggcc ccttgacaag gattacccgc gcgctataat
      181 ggtgtcatat tgatattcat caatacgtta ccttatgact gactccctcg actttgccaa
      241 ggccatggcc gatgaaaccc gacagcgcat catggcgctg ctctgctgcc gctggctgtg
      301 cgtgggcgat atcgtcactg agatgcaggc catcagccag ccaaccgtct cccaccacct
      361 ggccatcctg cgcgaggccg gtctggtgca cgtgcggcgc gacggcaagc aggtgttcta
      421 ctcgctgaac caggacgagg tcgccgtctg ctgtggccgc atcatgcgga acttcgcgcc
      481 ggaagttcca gtggcgggga tagccgtgtc tgagtaagtt atcagtctct ttttttaccc
      541 atacccatag atggatatcg atatgaggaa catagaatga cggatgacaa tacaattcga
      601 gaggcagtgc agtcccacta tgcggcagtg gccagcggtg gcagttgttg cggtgacagt
      661 tgctgcaccc ccggcgggga cgcggccgtc gatgtcagcc tggattcgct ctacggcaat
      721 ctctacacgg ccgacacgac ctggctgccg ccggcggtga ccggcctgtc gctcggctgc
      781 ggcgatccca tcaccctggc tggcctgcaa ccgggccaga cggtgctcga cctcggctcc
      841 ggcggcggca tcgactgctt catggccgcg cgccaggtgg ggccagacgg ccacgtgatc
      901 ggcgtggata tgaccccgga aatgctggcc caggcggagc gcaacaagag cacggtgggg
      961 ctggaaaacg tcgagttccg gcagggcgtc atcgaggccc tgcccgtggc cgacgggact
     1021 gtagacgtga tcctgtccaa ctgcgtgatc aacctcagcc ccgacaagcc ggctgtcttc
     1081 cgcgaggcct accgcgtgct gcggccgggt ggacggctgg cggtctcgga catgatgacc
     1141 gttggcctct tttcaccggc ggagcgcgcc gacctgtcgg cgtgggcggg ctgcgtcagc
     1201 ggcgcggagg atgtcgccca ctacgtcgcg gccatgcgcg cggctggctt cgaggacatc
     1261 tctatcgtgg acaaggccgc gcccggtgag gaactggccg ggcagctacc ggaaaccggc
     1321 ccggcgcgcc tcttcagcgc ccgcgtcacg gccgtgaaac actaggcagc cacaaaaaag
     1381 gacgccccac cgcgccggca taactggggc agtcgggcgt ccttaccttt caagtacgcg
     1441 ttatctggct tagaagggga tgtcgtcggc ttcctgagcc gccggccggc cgccgccgcg
     1501 ggcgggggca aagtggtgat tccgccgcgc gttccggacg
//
LOCUS       DAOSMQ010000633         1536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_81856_length_1536_cov_4.055368, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000633 DAOSMQ010000000
VERSION     DAOSMQ010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81856_length_1536_cov_4.055368"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PK829_14945"
     CDS             <1..348
                     /locus_tag="PK829_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42563.1"
                     /translation="IGVVLGVALIYGPLPLRWEAVHSDVGPNGFTDTQARGPLRAWQH
                     RHRFTALGPTQTRVDDHIIYSHDRGLRGVLSRLLFARPGLLYLFTARKVLTRRGVARL
                     LAERPAERGSGAG"
     gene            348..>1536
                     /locus_tag="PK829_14950"
     CDS             348..>1536
                     /locus_tag="PK829_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU42564.1"
                     /translation="MAAARAVIVGAGVGGLTTAALLLRAGYAVTVLEAHVYPGGSAGT
                     FYHKGYRFDAGATLAGGFSPGGPHARLAELLGLTWPIAPVDPAWVVHLPDGRAVTQWA
                     DPAAWQAERRRAFPGTESFWRQQERLADIAWDVSSRPFPWPPRALADLVTLGLALRPA
                     TLGALPYLHHTMADLVAGHAPDDDPLFRLFLDAQLLISAQTTAVHAGALYGSAALDLP
                     RRGVNHVRGGIGALAGTLVAWIRAHGGEVLTRQRVERIEAGRGRRAVVHTRRGLSLEA
                     DVVVANVTPWALCDLLGPDAPPALGKAVARLRPTWGAFTLYLGLDATALPASLPDHHQ
                     VVVDATKPLGEGNSVFLSLSDAADVERAPVGMRAMTLSTHTDVAAWWALRQEPDESAY
                     LARR"
BASE COUNT          190 a          559 c          547 g          240 t
ORIGIN      
        1 atcggcgtcg tgctgggggt ggcccttatc tacgggccgc tgccgctgcg ctgggaggca
       61 gttcacagcg acgtcggccc aaacggcttc accgatacgc aggcgcgcgg ccccttgcgc
      121 gcctggcagc accgccaccg cttcaccgcg ctcggcccca cgcagacgcg cgtcgatgac
      181 cacatcatct acagccacga ccgcggcttg cgtggcgtgc tcagccggct gctcttcgcc
      241 cggccggggt tgctctacct ttttacggcc cgcaaggtgc tgacgcggcg cggcgtggcc
      301 cggctgctgg ccgagagacc agcggagcgt gggagcgggg cgggttaatg gccgccgcgc
      361 gggcagtcat tgtcggcgcg ggcgtgggcg gcctgactac ggcggcgctg ctgttgcgcg
      421 ccggctatgc tgtcaccgtc ctagaggcgc acgtctatcc cggcggcagc gcaggcacgt
      481 tctaccacaa gggctaccgc ttcgacgctg gagcgacgtt ggctggcggc ttctcgcccg
      541 gcgggccgca cgcccgcctg gccgaactac tggggctaac ctggcccatc gcacccgtcg
      601 atcctgcctg ggttgtccac ctgcccgacg gccgcgccgt gacccaatgg gccgacccgg
      661 ccgcctggca agcggaacgg cggcgcgcct tccccggcac agagtcgttc tggcggcaac
      721 aggaacgcct ggctgatatc gcctgggacg tgtcctcacg cccctttccc tggccgccgc
      781 gcgcgctggc cgacttggtg actctggggc tggcgctgcg cccggccacg ctgggcgcgc
      841 tgccttacct gcatcacacc atggccgacc tcgtggccgg ccacgcgccc gacgatgacc
      901 cgctcttccg cctcttcctg gatgcgcaac tgctcatctc cgcccagacc acagctgtcc
      961 acgccggggc gctctacggc agcgccgcgc tcgacctgcc gcggcgcggt gtcaaccatg
     1021 tccgcggcgg catcggcgcg ctggcgggca cgctggtggc ttggattcgc gcccacggcg
     1081 gcgaagtgct tacgcggcag cgcgtcgaac gcatcgaggc ggggcgcggc cggcgcgccg
     1141 ttgttcacac gcgccgcggg ctgagtctgg aggcggatgt ggtcgtcgcc aatgtaacgc
     1201 cgtgggcgct gtgtgacctg ctggggccag acgcgccgcc ggcgctgggc aaggccgtag
     1261 cccggctgcg gccgacctgg ggcgcgttca cgctctatct ggggttggac gcgacggcgc
     1321 tgcccgcaag cttgcctgac caccaccagg tcgtcgtcga cgcgaccaaa cccctgggcg
     1381 agggcaactc cgttttcctg tcgctgtctg acgcggccga cgtggagcgc gccccggtgg
     1441 ggatgcgggc gatgacgctc tcgacgcaca cagatgtagc cgcgtggtgg gcgctgcggc
     1501 aggaaccgga cgagagcgcc tatctggccc ggcgcg
//
LOCUS       DAOSMQ010000634         1535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_81927_length_1535_cov_4.408108, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000634 DAOSMQ010000000
VERSION     DAOSMQ010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81927_length_1535_cov_4.408108"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..>1535
                     /locus_tag="PK829_14955"
     CDS             167..>1535
                     /locus_tag="PK829_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOU42565.1"
                     /translation="MLAKVLSAAIVGLEAELVNVEVDIVNGQPYFALVGLPDAAVRES
                     RDRVYAAVKNSGLNFPQHRITVNLAPADLRKEGPAYDLPIAVGMLAATAQIWHNELDD
                     SLFFGELSLDGSTRHTKGVLPLAALAREKGLKRVFVPAADAAEAALMPDIAVLPVRNL
                     GELVGHLTGLALLSPVTVDLQAAFDGEVAYTTDFADIAGQEHVKRAMEIAAAGGHNAL
                     MTGPPGAGKTLIARALPSILPRMGVDEALEVTKIYSVAGMLPAETPLIRTRPFRSPHH
                     TISYAGLVGGGQWPRPGEISLAHRGVLFLDELPEFGERLIEVLRQPLEGVPREVSISR
                     ATGSVTYPANFMLIAAQNPCPCGYYGDPTRACTCSNTMITRYQKRVSGPLMDRIDLHV
                     DVPRVEYEKLSSNQRGEQSAAVRARVEAARQRQAQRFAGTTLYSNADMGPGEVRQFCD
                     LDDTGR"
BASE COUNT          256 a          562 c          486 g          231 t
ORIGIN      
        1 ccattctacc agcttcgcgg cggggtcgaa gcgccgaccg ctgaccacag accattgacc
       61 tactgacgac tgacccactg tctactgtcg actgtccact gtccactgcc cactaattcc
      121 cctgtcgtaa tctgtggcga aagcagcgct gcgctataat caggacatgc tcgccaaagt
      181 cctcagcgct gccatcgtcg ggctggaagc cgaactcgtc aacgtcgaag tcgatatcgt
      241 caacggccaa ccctatttcg ccctcgtcgg tctgcccgat gccgccgtgc gcgaaagccg
      301 cgaccgggtc tatgccgcgg tcaagaacag cggcctgaac ttcccccagc accgcatcac
      361 cgtcaacctg gccccggccg acctgcgcaa ggaaggcccg gcctatgacc tgcccatcgc
      421 cgtgggcatg ctggcggcca cggcgcaaat ctggcacaac gaactggacg actcgctctt
      481 cttcggcgag ctgtcgctcg acggctcgac acgccacacc aagggcgtgc tgccgctggc
      541 ggcgctggcc cgcgagaagg ggttgaagcg cgtcttcgtg cccgccgccg acgcggccga
      601 ggcggcgctg atgcccgaca tcgctgtcct gcccgtgcgc aacctgggcg aactggtcgg
      661 ccacctgacc ggcctggccc tcctctcgcc cgtgaccgtc gatctgcaag ccgccttcga
      721 cggcgaagtg gcctacacaa ccgacttcgc cgacatcgcc gggcaggagc acgtcaagcg
      781 ggcgatggag atcgccgccg ccggcggcca caacgccctc atgaccggcc cgccgggcgc
      841 gggcaagacg ctgatcgctc gcgccctgcc cagcattctg ccgcgcatgg gcgtcgatga
      901 ggcgctggaa gtgaccaaaa tctacagcgt cgccgggatg ctgccggcgg agacgccgct
      961 catccgcacc cggccgtttc gctcgcccca ccacacgatc agctacgctg gcctggtggg
     1021 cggcgggcag tggccgcggc cgggggaaat ctcgctggcc catcgcggcg tgctctttct
     1081 ggacgagttg ccggagttcg gcgagcggct gatcgaagtg ctgcgccagc cgttggaggg
     1141 cgtgccgcgc gaggtcagca tcagccgggc cacgggcagc gtcacctacc cggccaactt
     1201 catgctcatc gccgcccaga acccctgccc ttgcggctac tatggcgacc cgacgcgggc
     1261 ctgcacctgt tccaacacga tgatcacccg ctaccagaag cgcgtcagcg ggccgctgat
     1321 ggatcgcatc gacctgcacg tggacgtgcc gcgggtggag tacgaaaagc tgtcgtcgaa
     1381 tcagcgcggc gagcagtcgg cggccgtccg cgcccgcgtc gaggccgccc gccagcgaca
     1441 ggcgcagcgc ttcgccggga cgacgctcta cagcaacgcc gacatggggc cgggcgaggt
     1501 gcgccagttc tgcgacctgg acgacaccgg ccgcc
//
LOCUS       DAOSMQ010000635         1530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_82310_length_1530_cov_5.575593, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000635 DAOSMQ010000000
VERSION     DAOSMQ010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82310_length_1530_cov_5.575593"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PK829_14960"
     CDS             <1..1249
                     /locus_tag="PK829_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU42566.1"
                     /translation="RLARLYATSTPSLIKIADGLQRNPMGGQTTRAICALPALTGQYG
                     VRGGGLAYSTSGYVQWDSEAVHKWAECPPPGRVVNMNRLGAALLGEVTDPPIMALYVF
                     GSNPAAIAPNAGAVVAGLMRADLFTVVHELFLTDTADYADIVLPATSNLEQADLHKAY
                     GHTLLRYNHPAIPPLGQSKSNWDVMRLLAAEMGFDEPWLRQDVEAVIDEVLTASAARS
                     PFLAGITLARLKSEGQVALNVGPDAPFAGGHFPTPSGKVELYSARLAAEGHDPLPNVT
                     GVWDDGAPSPDWRPEEALDLITPAAHHFVTSSFGNGRSQLRGEGGPTIEIHPADAAAR
                     GIRHGELVIVENGRGAVTLRAVVSDGVRPGVVAAAKGRWSKRGDGRGDRRNVNWTTSD
                     ALGDFAGQSTFHSNRVWLRKERD"
     gene            1331..>1530
                     /locus_tag="PK829_14965"
     CDS             1331..>1530
                     /locus_tag="PK829_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42567.1"
                     /translation="MDFQAPAIDYLVLQPLLWVVGAGMLIMLLDLLLDGDRVRRAAPI
                     VAMIGLFGGLITSAALWNSNRAA"
BASE COUNT          246 a          516 c          529 g          239 t
ORIGIN      
        1 gcggctggcc cggctctacg ctaccagcac gcccagcctg atcaagatcg ccgacggttt
       61 acagcgcaac ccgatgggcg ggcagacgac gcgggccatc tgcgccctgc cggccctgac
      121 ggggcagtac ggcgtgcgcg gcggcggctt ggcctatagc accagcggct acgtccagtg
      181 ggacagcgag gcggttcaca aatgggctga gtgcccgccg cccggccgcg tcgtcaacat
      241 gaaccgcctg ggcgcggcgc tgctgggcga agtgaccgat ccgccgatca tggcgctcta
      301 tgtcttcggc tccaacccgg cggccattgc ccccaacgcc ggcgcggtcg tggccgggct
      361 aatgcgcgca gacttgttta ccgtcgtgca tgaactgttc ctgaccgaca cggccgacta
      421 tgccgacatc gtcctgccgg ccacgtcgaa cctggagcag gccgacctgc acaaggccta
      481 tggccacacg ctgctgcgct ataaccaccc ggccatcccg cccctgggcc agagcaagag
      541 caattgggac gtgatgcgcc tgctggcggc ggagatgggc ttcgacgagc cgtggctgcg
      601 gcaggatgtc gaggctgtca tcgacgaagt gctgacggcg tcggcggcgc ggtcgccctt
      661 tctggcgggc atcactctgg cgcggctgaa gagcgaggga caggtggcgc tgaacgtcgg
      721 ccccgacgcg cccttcgccg gcggccactt tcctactccc agtggcaaag ttgagctata
      781 ctcagcgcga ttggcggccg agggccacga cccgctgccg aacgtcaccg gggtgtggga
      841 cgacggcgcg ccctcgccgg actggcggcc cgaagaggcg ctcgacctga ttacgcccgc
      901 ggcccaccac ttcgttacca gcagcttcgg caacggccgc agccagctcc ggggcgaggg
      961 cggaccgacg attgagattc accccgccga cgccgccgcg cgcggcatcc ggcacgggga
     1021 gctggtcatt gtggagaacg gccgcggcgc agtaaccctg cgagccgtgg tcagcgacgg
     1081 cgtgcgcccc ggcgtggtcg ccgccgccaa ggggcgctgg agcaagcgcg gtgacggccg
     1141 cggcgaccgg cgcaatgtca actggacgac ttccgatgcc ctgggcgact tcgccgggca
     1201 gagcacgttt catagcaacc gggtctggct gcgcaaagaa agggactagg ggttaggggc
     1261 tagggactag gaagagcttt cttcccctcg tccctagccc ctaatccctg acccctaaga
     1321 aggtgggaca atggactttc aagcgcctgc gattgattat ctggtgctac agcctttgtt
     1381 atgggtggtg ggggcgggca tgctcatcat gctgctcgac ctgctgctcg acggcgaccg
     1441 cgtgcgccgc gccgcgccca tcgtggccat gatcggcctg ttcggcgggc tgatcacctc
     1501 ggccgcgctg tggaacagca accgggcggc
//
LOCUS       DAOSMQ010000636         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_82566_length_1527_cov_2.995924, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000636 DAOSMQ010000000
VERSION     DAOSMQ010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82566_length_1527_cov_2.995924"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PK829_14970"
     CDS             <1..661
                     /locus_tag="PK829_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42568.1"
                     /translation="AFEQPHDGGFDVLVVRYNGFDPGDFSSFSVDVDPTSIKGVGAPG
                     PGESGSVGGLELVGATVTATFDNGATITGQVYRLADDGGLGGHHSGGTVVLRDGRPPR
                     PQISVLGVTPPATVTNANQTVRVVGPPGQPVTVLVVEGGLFLAGVPNGGFDIDPFEAN
                     NAVDVREYQAVVGGSGSVDVPIVLMKQNDKSGIHAITAVFDNHYGLKGLVAAPLVVEL
                     E"
     gene            680..>1527
                     /locus_tag="PK829_14975"
     CDS             680..>1527
                     /locus_tag="PK829_14975"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOU42569.1"
                     /translation="MHKDRINRAGRSPAGDRPALQGEPSMTTSSSSARRLWPVLALCL
                     LAVVVLLAAPGARAAAGPNRIFMPLVRVAAPPVFEVSPLVDLGSITITDIANAGDERL
                     FVADREGRVYIVYPNGTVAPELFMDARHLVQLGSDGNENDFEQGFLGLAFHPNYPATP
                     YIYFAHTTPRTLTVTRVTLSATNPNKVNPTTFVPLIFERKPDKDAAGNLSAVHNAGDL
                     AFGPDGYLYIPIGDGGPDPYDPWGVPGDPNNHSQRFDTIFGSILRIDVDPTRGAPADC
                     GVKGRYS"
BASE COUNT          241 a          540 c          502 g          244 t
ORIGIN      
        1 cgcctttgag cagccccacg acggcggctt cgacgtgctg gtggtgcgct acaacggctt
       61 cgaccccggc gacttctcgt cgttctccgt cgacgtcgat ccgacgagca tcaagggcgt
      121 cggcgcgccc ggcccgggtg agtcgggtag tgtcggcggg ctggagcttg tcggggcaac
      181 ggtgacggcc acgttcgaca acggcgcgac gatcaccggc caggtctacc ggctggccga
      241 cgacggcggg ctgggtggcc accacagcgg cggcacggtc gtgctgcgcg atgggcggcc
      301 gccgcggccg cagatcagcg ttctgggcgt cactccgccg gcgaccgtga ccaacgccaa
      361 ccagaccgtg cgcgtcgttg gcccgcccgg ccagccggtg acggtgctgg tggtcgaggg
      421 cgggctgttc ctggcaggcg tgcccaatgg cggctttgat atcgacccgt tcgaggctaa
      481 caacgccgtc gatgtgcgcg agtatcaggc cgtcgtcggc ggcagcggca gcgttgacgt
      541 gcccatcgtc ctgatgaagc agaacgataa gagcggcatc cacgccatca cggccgtctt
      601 cgacaaccac tatgggctga aggggctggt ggccgcgccg ctggtcgttg aactggagta
      661 ggccgccagt tgtgatagaa tgcacaagga cagaatcaac cgcgccggcc ggtcgcccgc
      721 gggcgaccgg ccggccctgc aaggagaacc atccatgacc acatcctcct cctccgcgcg
      781 ccggctgtgg ccggtgttgg cgctgtgcct gttagctgtc gtcgtccttt tggccgcgcc
      841 cggagcgcgc gcggccgccg ggccgaacag gatctttatg ccgctggtgc gcgtggccgc
      901 gccgccagtg ttcgaggtct cgcccctggt cgatctgggc agcatcacca tcaccgacat
      961 cgccaacgcc ggcgacgagc gcctgttcgt cgccgaccgc gaggggcgag tctacatcgt
     1021 ctaccccaac ggcacggtcg cgccggagtt gttcatggat gcgcgccatc tggtgcagtt
     1081 gggcagcgac ggcaatgaga acgacttcga gcagggcttc ctcggcctgg cttttcaccc
     1141 caactacccg gctacgccct acatctactt cgcccacacg acgccgcgca cgctcaccgt
     1201 cacccgcgtg accctgagcg cgacgaaccc caacaaggtc aacccgacga ccttcgtgcc
     1261 cctcatcttt gagcgcaagc cggacaagga cgccgccggc aacctgagcg ccgtccacaa
     1321 cgccggcgac ctggcctttg ggccggacgg ctacctctac atccccatcg gtgacggcgg
     1381 gccggaccca tatgacccgt ggggcgtgcc cggcgacccc aacaaccatt cccagagatt
     1441 cgacaccatc ttcggctcca tcctgcgcat cgacgtggac ccgacgcgcg gcgcgccggc
     1501 cgattgcggc gtcaaggggc gctactc
//
LOCUS       DAOSMQ010000637         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_83854_length_1510_cov_5.407560, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000637 DAOSMQ010000000
VERSION     DAOSMQ010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83854_length_1510_cov_5.407560"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1510)
                     /locus_tag="PK829_14980"
     CDS             complement(<1..>1510)
                     /locus_tag="PK829_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU42570.1"
                     /translation="PVPALRPSLDLCKPTGVTLDANSRVVVMLDRGGVGKALVSRLEK
                     LGATALVIDEPPTAEALAAQLAAWLADGPIQGVYWLPALDAEPALEDMDLATWRELNR
                     VRVKNLYTTMRALYDAISAPGTFLVSATRLGGLHGYGPDGATAPLGGAVTGFTKAYKR
                     ERGDVLVKAVDFEAGRKTAAPADALIAETLTDPGIVEVGYAGDDRYTVTLAEQPAADG
                     GEGLTLNKDSIFVVTGAAGGITSAIVADLAAASGGTFYLLDLVPEPAQDDQQIALFRQ
                     GKDALKAHLIAAAKASGEKVTPAQIDKQIMAVERNEAALRAIEAVEAAGGRAYYRNAN
                     LLDGPALTAIVDEIRERHGRIDVLVHAGGIEISRSLPDKDYNQFSLVFDIKADGFFSL
                     LNAAKGLPIGATVAFSSVAGRFGNNGQTDYSAANDLLCKLTSSLRRWRPQTKGLVIDW
                     TAWGEIGMATRGSIPRIMEMAGIDMLPPSAGVPTVRRELVAGCTGPASGRPSC"
BASE COUNT          216 a          508 c          540 g          246 t
ORIGIN      
        1 gcaggacggc cggcccgacg cgggccccgt gcagccggct accagctcgc gccgcaccgt
       61 gggcacgccg gccgacggcg gcagcatatc gatgccggcc atctccatga tgcgcgggat
      121 agagccgcgg gtagccatgc caatctcgcc ccaggccgtc cagtcgatga ccaggccctt
      181 cgtctgcggc cgccaacggc gcaggctgct ggtcaacttg cagagcaggt cgttggcggc
      241 gctgtagtcg gtctggccgt tgttgccgaa gcgcccggcg accgagctga aggccaccgt
      301 cgcgccgatg ggcaggccct tggccgcgtt cagcaggctg aagaagccgt cggccttaat
      361 gtcaaagacg aggctgaact gattgtagtc cttgtccggc aggctgcggc tgatctcgat
      421 gccgccagca tggacgagca catcaatacg gccgtggcgc tcgcgaatct cgtcgacgat
      481 ggcggtcagg gccggcccgt cgagcagatt ggcgttgcgg tagtaggccc ggccgccggc
      541 ggcctcgacc gcctcgatgg cccgcagggc ggcctcgttg cgctccacgg ccatgatctg
      601 cttgtcgatc tgcgccggag taaccttctc gccgctggcc ttagccgcgg cgatgaggtg
      661 cgctttgagg gcatccttac cctggcggaa gagggcgatc tgctggtcgt cctgagccgg
      721 ttcgggcacc aggtccagca gatagaacgt accgccgctg gccgcggcca ggtcggcaac
      781 gatggcgctg gtgatgccgc cggccgcgcc ggtgacgacg aagatgctgt ccttattcag
      841 agtcaggcct tcgccgccgt cggccgctgg ttgctcggcc agcgtgacgg tgtagcggtc
      901 gtcgccagca tagccgactt cgacgatgcc ggggtcggtc agcgtctcag caataagggc
      961 gtcggccggg gcagctgtct tgcgacccgc ctcgaagtcg acggccttga cgaggacatc
     1021 gccgcgctcg cgcttgtagg ccttggtgaa gcccgtcacc gcgccgccca gcggggccgt
     1081 cgcgccgtcg gggccatagc cgtgcaagcc gcccagccgt gtggccgaga cgaggaacgt
     1141 gcccggcgcg ctgatggcgt cgtagagcgc gcgcatggtc gtgtagaggt tcttgacgcg
     1201 cacgcggttg agttcgcgcc acgtggccag gtccatgtcc tccagcgccg gttcggcgtc
     1261 gagggcgggc agccagtaga cgccctggat gggaccgtcg gccagccagg cggccagttg
     1321 tgccgccagc gcctcggccg tcggtggctc gtcgatgacc agcgccgtcg cgccaagctt
     1381 ctccagccgg cttaccaacg ccttgcccac gccaccgcgg tcgagcatga ccacgacgcg
     1441 gctgttggcg tcgagtgtca cgcctgtcgg cttgcatagg tcgagcgacg gccgcagcgc
     1501 cgggacgggg
//
LOCUS       DAOSMQ010000638         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_84541_length_1502_cov_2.521769, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000638 DAOSMQ010000000
VERSION     DAOSMQ010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84541_length_1502_cov_2.521769"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PK829_14985"
     CDS             <1..613
                     /locus_tag="PK829_14985"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU42571.1"
                     /translation="IGRSAGNELVLADPEVSRRHARVLRRADGYAVEDIGSTNGTFVN
                     GQRIGHLTLLQDGDTIDLGDTVRLRFVAPVIAPSPIPPAPVDMSERPTEMLAAAPAAM
                     PAWPRPAEQPVPAYVPPAAVAPMNVQPAAPPPPVYPPSYPVAPRRGRGLLIGCGLLIA
                     LLLVCAGTFLILDAYDQGRLLYCGPAQPVIELVLGTARSATCP"
     gene            635..>1502
                     /gene="amrB"
                     /locus_tag="PK829_14990"
     CDS             635..>1502
                     /gene="amrB"
                     /locus_tag="PK829_14990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU42572.1"
                     /translation="MIGQQTTPISNPHLRLLDFQPVQQYGESMWLLRDPWQLGERQLI
                     FPAALAQMLPLCDGTRSPRQLQSDLSEDLGVPVPFDIISEALAQLDAAYLLHNDRYEA
                     RRQELLDVYRAQPFRPPALAGLSYPAAPAELSAQLRGYGHGDSATVAEWQGQGIVSPH
                     IDYQRGGPVYARVWRRAAPAVLAADLVLIFGTDHYGGPASITPTPLPYATPFGVLPTD
                     RALIDRLAEALGPEAAYAEELNHRQEHSVELSAVWLHHIFREAGVAPCPVVPILIGSF
                     HHFVTSGNHPSDE"
BASE COUNT          219 a          577 c          458 g          248 t
ORIGIN      
        1 catcggccgc agcgccggta acgaactggt gctggccgat ccggaagtct cgcgccgcca
       61 cgcccgcgtg ctgcggcgcg ccgacggcta tgccgtggag gacatcggca gcaccaacgg
      121 cacgttcgtc aatggccagc gcatcggcca cctgacgctg ctgcaagacg gcgacaccat
      181 cgacctgggc gatacggtgc gtctgcgctt tgtcgccccc gtcatcgccc catcccccat
      241 tccgcccgcg ccggtggata tgtccgagcg gccgaccgag atgctggccg ccgcgccggc
      301 agcgatgcca gcatggccgc gcccggccga gcagcctgtg ccggcctacg ttccgccggc
      361 ggctgttgcg cccatgaacg tccagccggc cgcgccgccc ccacccgtct atccacccag
      421 ctatccggtc gcgccgcgcc gtggccgcgg cctgctcatc ggctgtgggt tgttgatcgc
      481 cctgttgctg gtctgcgccg gcacctttct gatcctggat gcctatgacc agggccgtct
      541 gctctattgt ggcccggccc agccggtcat cgaactggtg ctgggcacgg ccagatcggc
      601 cacctgcccg taacgccccc aggggatcgc ccttgtgatt ggccagcaga caacgcccat
      661 tagcaacccg cacttgcggc tgctcgactt ccagcccgtc cagcaatacg gcgagtcgat
      721 gtggctgcta cgtgacccgt ggcagctggg cgaacggcag ctcattttcc cggcggccct
      781 ggcccagatg ctgcccctgt gcgacggcac gcgcagcccg cgccagctcc aaagcgacct
      841 gagcgaggat cttggcgtgc ccgtgccttt cgacatcatc agcgaggccc tggcccaact
      901 cgacgccgcc tatctgctgc acaacgatcg ctatgaggcg cgccggcaag agttgttaga
      961 cgtctaccgc gctcagccct tccgcccacc ggccctggcc gggctgagct acccggcagc
     1021 gccggccgag ctaagcgcgc aactgcgcgg ctatggccac ggcgacagcg ctacggtggc
     1081 tgaatggcaa ggacaaggga tcgtctcgcc ccatatcgac taccagcgcg gcgggcccgt
     1141 ctatgcccgc gtgtggcggc gggccgcgcc ggccgtgttg gccgccgatc tggtgctcat
     1201 cttcggcacc gaccactacg gcggcccggc cagcatcacc cccacgccac tgccctatgc
     1261 cacccccttc ggtgtcctgc cgaccgatcg cgccctcatt gaccgcttgg ccgaagccct
     1321 ggggccagaa gcggcttatg ccgaggagtt gaaccaccgc caggagcact ctgtagaact
     1381 gtcggccgtc tggctgcacc acatcttccg cgaggcgggc gtggccccct gcccggtcgt
     1441 gcccatcctg atcggctcct tccaccattt cgtcacctcc ggcaaccacc cctctgacga
     1501 ac
//
LOCUS       DAOSMQ010000639         1492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_85278_length_1492_cov_4.720946, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000639 DAOSMQ010000000
VERSION     DAOSMQ010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85278_length_1492_cov_4.720946"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PK829_14995"
     CDS             complement(<1..645)
                     /locus_tag="PK829_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU42573.1"
                     /translation="MEPNLIDETWAAELAAGPLAGLTALERDIFLTRLRLYWADALRP
                     LRRLYGDRPDFDDCCRRLLAIVAAGYAARPAELRLLDLQRQGEPDWFQQPSMLGYVAY
                     ADRFAGDLRGVAARIPYLVELGVTYLHLMPLLMPRQGPNDGGYAVADYRAVNPALGTM
                     ADLAALAGQLRAEGISLCIDLVCNHTAKEHEWARRAVAGDPTYQAYYLMYPDRAE"
     gene            complement(662..>1492)
                     /locus_tag="PK829_15000"
     CDS             complement(662..>1492)
                     /locus_tag="PK829_15000"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HOU42574.1"
                     /translation="EFQVCFHFPIMPRLYMALKAADKTPIINILADTPPIPAGTQWMT
                     FLRNHDELTLEMVTPEERQWMWANYAAEPRARLNLGIRRRLLPLLEGDKRRWLALNAM
                     FLSLLGTPILYYGDEIGMGDNIWLDDRDGVRTPMQWADAPNAGFSTGRKTYLPVIDQA
                     PYDYRHVNVAAQENDPDSYLAWMRLLVAARQSQPSLRSGEREWVETGDVAVLAYWRRG
                     VGEPVLCVFNLSDDPRPAGIVPERDMRDLLAREGRVYPAGQAIALAPYAALWLVGAGA
                     "
BASE COUNT          236 a          520 c          521 g          215 t
ORIGIN      
        1 ctcggcgcgg tcgggataca tcaggtagta agcctgataa gtcgggtcgc cggccacggc
       61 ccgccgcgcc cactcgtgct ccttagcggt gtggttacag accaggtcga tgcacaggct
      121 gataccctcg gcccgcaact ggccggccag cgcggccagg tcggccatcg tgcccaacgc
      181 cgggttgacg gcgcggtagt cggccacagc atagccgcca tcgttggggc cttggcgcgg
      241 catcagcaga ggcatcaggt gcaggtaggt gacgcccaac tcgaccaggt agggaatgcg
      301 cgcggccaca ccgcgcaggt cgccggcgaa gcggtcggcg taggcgacgt agcccagcat
      361 cgacggctgc tggaaccagt ccggctcgcc ctggcgctgc aagtcgagca ggcgcaactc
      421 ggccggccgg gcggcgtagc ccgcggcgac aatggccagc agccgccggc agcagtcgtc
      481 gaagtccggc cggtcgccgt agagacggcg cagcggccgc agcgcgtcgg cccagtatag
      541 ccggagacgc gtcaggaaga tgtcgcgctc cagggccgtc agacccgcca gcggcccggc
      601 ggctaactca gcggcccagg tttcgtcgat caggtttggc tccacgccag acactcccac
      661 ctcaagcgcc cgcgcccacc agccacagcg cggcataggg agccagcgcg atggcctggc
      721 cggccggata gacgcgcccc tcgcgcgcga gcaggtcgcg catatcccgc tccggaacga
      781 tgccggccgg ccgtgggtcg tcgctcaggt tgaagacgca cagcaccggc tcgcccacac
      841 cgcgccgcca gtaggccagc acggccacgt cgcccgtctc cacccactcc cgttcgccgc
      901 tgcgcaggct cggctgcgat tggcgcgccg ccaccagcaa gcgcatccag gccaggtagg
      961 agtcggggtc gttctcctgg gcggcgacgt tgacgtggcg atagtcatag ggcgcctggt
     1021 cgatgaccgg caggtaggtc ttgcggcccg tcgagaagcc ggcgttgggc gcgtcggccc
     1081 actgcatcgg cgtgcgcacg ccgtcgcggt cgtctagcca gatgttgtcg cccatgccga
     1141 tctcgtcgcc gtagtagagg atgggcgtgc ccagcagcga cagaaacatc gcgttcagcg
     1201 ccagccagcg gcgcttgtcc ccctccagca gcggcagcag ccgccggcgg atgcccaggt
     1261 tcagccgggc gcgcggctcg gcggcgtaat tggcccacat ccactgccgt tcctccggcg
     1321 tgaccatctc cagcgtcagc tcgtcgtggt tgcgcaggaa ggtcatccac tgcgtgcccg
     1381 ccggaatggg cggcgtgtcg gccaggatgt tgatgatggg tgtcttgtcg gccgccttca
     1441 gggccatgta tagccgcggc atgatcggga agtggaagca gacctggaac tc
//
LOCUS       DAOSMQ010000640         1490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_85468_length_1490_cov_3.035540, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000640 DAOSMQ010000000
VERSION     DAOSMQ010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85468_length_1490_cov_3.035540"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1490
                     /locus_tag="PK829_15005"
     CDS             <1..>1490
                     /locus_tag="PK829_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42575.1"
                     /translation="KVVDATTNLTGNSFMGTPAYMSPEQFTGGALDGRSDQYSLAVVA
                     FEALSGRVPFAAETIHGLMYQHVHEPPPDIHTIDKSLPPGVSATLRRALAKRPEDRFA
                     SVSDFIEALRLAATPGAVAAQTQQLQSYYDSGRYAYERGDWPTAVDFLGRVVAADAGY
                     RDANQLYKTAVMRTQAGAPPAVAAPQPPIVREAGSPVAAPAPAGKSRLPLLLGLLALL
                     IIGGAAAFFFLRPDAAPEATPVAVVAPTEVATEAPTATPAPTDTPLPTELPPIAGAAR
                     VVEAGAASYRVDGGSAQPLPNDTALPLELNRTVNVAVGEGRLQLVLPDGTRLYLDEGA
                     EISLTADRAGVPFVTVEEGRVLAWGHTDGIELTSALDGRAALLGPGAMGVNMLPGRLS
                     FEVACLDGSCDVSGAEDTAVLRLRNGQQSVIGGDGFAAPPARADRASYLALAPEVFGG
                     PDAVTQAAVTEEATLAPTETATPTEAPTATPTATLAPTTRPNPNGG"
BASE COUNT          211 a          562 c          523 g          194 t
ORIGIN      
        1 ccaaggtcgt cgatgcgacg acgaatctga ccggcaacag cttcatgggc acgccggcct
       61 acatgagtcc ggagcagttc accggcgggg cgctcgacgg ccgctccgac cagtatagcc
      121 tggccgtggt cgccttcgag gcgctcagcg gacgagtgcc cttcgccgcc gagacgatcc
      181 acggattgat gtaccagcac gtccacgagc cgccgcccga catccacacg attgataagt
      241 cgctgccgcc gggcgtgtcg gctacgctgc gacgggcgct ggccaagcgg ccggaagacc
      301 gcttcgccag cgtcagcgat ttcatcgagg cgctgcggct ggcggccacg ccgggcgcag
      361 tcgccgccca gacgcagcag ttgcaaagct actacgacag cggacgctac gcctacgagc
      421 gcggcgactg gccgacggcg gtcgatttcc tcggccgcgt cgtcgccgcg gacgccggct
      481 accgcgacgc caaccagctc tacaagacgg cggtcatgcg cacgcaggcg ggggccccgc
      541 ctgcggtcgc cgcgccccag ccgccgatcg tccgcgaggc gggcagcccc gtggccgcgc
      601 ctgccccggc gggcaagagc cggctgccgc tgttgctggg gctgctggcg ctgctgatca
      661 tcggtggcgc ggcggcgttc ttcttcctgc ggcccgacgc agccccggag gccacgccag
      721 tggctgtcgt cgcgccgacg gaagtcgcca cggaagcgcc cacggccacg cccgcgccca
      781 ccgacacccc tctgccgacc gagctgccgc ccatcgccgg cgcggcccgc gtcgtcgaag
      841 ccggcgcggc cagctatcgg gtcgatggcg ggtcggccca gccgttgccc aacgacactg
      901 ccctgccgct ggaactaaat cgcaccgtca acgtcgccgt cggcgagggg cggctgcaac
      961 tggtgctgcc cgatggcacg cggctctacc tggatgaggg cgcggagatc agtctgaccg
     1021 ccgaccgcgc cggcgtgccg ttcgtcaccg tggaagaggg gcgggtgctg gcctggggcc
     1081 acacggacgg gatcgagcta accagcgccc tcgacggccg ggccgccctt ctcggcccgg
     1141 gcgcgatggg cgtcaacatg ctgcccggcc ggctgagctt cgaggtggcc tgtttggacg
     1201 gtagctgtga tgtcagcggg gccgaggaca cggctgtcct gcgcctgcgc aatggccagc
     1261 agtcggtcat cggcggcgat ggtttcgccg cgccgccggc ccgcgctgac cgcgcgtcct
     1321 atctcgccct ggccccggag gtgttcggag ggccggacgc ggtgacccag gcggctgtca
     1381 ccgaggaagc gacgctcgcg ccgaccgaga cggccacgcc gactgaggcc cccaccgcca
     1441 cgcccacggc gacgctcgct cccaccaccc gcccgaaccc caacggcggc
//
LOCUS       DAOSMQ010000641         1488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_85595_length_1488_cov_4.632938, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000641 DAOSMQ010000000
VERSION     DAOSMQ010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85595_length_1488_cov_4.632938"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK829_15010"
     CDS             <1..629
                     /locus_tag="PK829_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42576.1"
                     /translation="AAAQGARDLAIEVDAGNQTALRFLLSQGYRFRGDVWALKLPVEA
                     ELPAPVWPAGYSVRTYAEVDDLPLYTALNNRVFGDLWGHWENTPGLVDETHMAEVLTE
                     FDPRGIFLVFDAGGAAVGECRARAFPLDAHPRAPHILDQPGVAPDHRAAGLHVPLALT
                     AAHWLRAQAARPIRLESWGDAAATIDAYRALGFKPVEHEVSYVLDVSR"
     gene            626..1123
                     /locus_tag="PK829_15015"
     CDS             626..1123
                     /locus_tag="PK829_15015"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU42577.1"
                     /translation="MSVIIRPMQPADLDDAKRVILSVAAPMFGPDDPEGFIARWWTLL
                     TDVDDWPRVYGPPRGTFLVALDGERFIGTGAIRPLDETTAELKRLWLLEAYHGRGIGY
                     RITQELLAFARAAGYQRVWLITDRVEQGRAIRFYERLGFHDIPPYNDSEDTMFMELLL
                     SPEEK"
     gene            1142..>1488
                     /locus_tag="PK829_15020"
     CDS             1142..>1488
                     /locus_tag="PK829_15020"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU42578.1"
                     /translation="MTHTRINPPALFDSTRYGFSQVVVATGRRTVYCSGQVAWDANED
                     IGSAGMGEQARRALANVERAVGAAGGGLADVVSLRLYIVRQGEGDVRAIREALLAAFP
                     AGAQPATTWIAVAG"
BASE COUNT          210 a          513 c          533 g          232 t
ORIGIN      
        1 gggcggcggc gcagggcgcg cgcgacctgg ctatcgaggt ggacgccggc aaccagacgg
       61 cgctgcgctt tctgctgtcg cagggctatc gcttccgcgg cgacgtgtgg gcgctgaagc
      121 tgccggtcga ggccgagttg cccgcgccgg tctggccggc cggctactcg gtgcgaacct
      181 atgccgaagt ggacgacttg cccctctata ccgcgctcaa caaccgcgtc tttggcgacc
      241 tgtggggcca ttgggagaac acgcccggcc tggtcgatga gacccacatg gccgaggtgc
      301 tgaccgaatt cgacccgcgc ggcatctttc tggtcttcga cgccggtggc gcggccgtgg
      361 gcgaatgccg cgcccgagcc ttcccgctcg acgcccaccc ccgcgcgccc cacatcctcg
      421 accagccggg cgtggcccca gaccaccgcg ccgccgggct gcacgtgccg ctggcgctga
      481 cggcggccca ctggctgcgg gcgcaggcgg cgcggcccat tcgcctggag tcgtggggcg
      541 acgccgcggc gacgatcgac gcctaccggg cgctgggctt caagccggtg gagcacgagg
      601 tcagctacgt cctggacgtg agccggtgag cgtcatcatc cggccgatgc agccggccga
      661 tctcgacgac gccaagcgcg tcatcctgag tgtcgccgcg cccatgttcg gcccagacga
      721 ccccgagggc tttatcgccc gttggtggac gctgctgacc gacgtggacg actggccgcg
      781 cgtctacggc ccgccgcggg gcacgtttct ggtggccctc gatggtgaac ggttcatcgg
      841 cacgggggcg atccggccgc tggacgagac gacagccgag ctaaagcgcc tgtggctgct
      901 ggaagcgtat cacggccgcg gcatcggcta ccgcatcacg caggaactgc tggcttttgc
      961 ccgcgcggcc ggctatcagc gggtgtggtt gatcaccgat cgcgtggagc agggtcgcgc
     1021 cattcgcttt tacgagcgtc tcggcttcca cgacatcccg ccctacaatg acagtgagga
     1081 caccatgttt atggagttgc tgctcagccc cgaggagaag tagacccgct aaggaggaac
     1141 aatgacccat acccgcatca acccgccggc gctgttcgac agcacgcgct acggcttctc
     1201 tcaggtcgtc gtcgccaccg ggcggcggac ggtctactgc tccgggcagg tggcctggga
     1261 cgccaacgag gacatcggca gcgccggcat gggcgaacag gcgcggcggg cgctggccaa
     1321 cgtcgagcgg gctgtgggtg ccgctggtgg cgggctggcc gacgtcgtct cgctgcggct
     1381 ctacattgtc cgtcaggggg agggcgacgt gcgggccatc cgcgaggcgc tgctggccgc
     1441 cttcccggcc ggcgcccagc cggcgacgac gtggatcgcc gtggccgg
//
LOCUS       DAOSMQ010000642         1463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_87793_length_1463_cov_3.657670, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000642 DAOSMQ010000000
VERSION     DAOSMQ010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87793_length_1463_cov_3.657670"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PK829_15025"
     CDS             <1..521
                     /locus_tag="PK829_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU42579.1"
                     /translation="IGGRHYGVYGHDWRVTPPLAWLELLAERELSVGSRPAARPAAEP
                     IIVLSEAEFATAVRDALRDYTDTAALLANPLLRSRLVLGQAGTDTTAAARIAALRKLL
                     REAAESLQDSPKLVKFHRALYHTYLQPAATQEQAAEVLDLPFSTYRRHLRSGIEEVTG
                     RLWLREIGNLEA"
     gene            complement(529..936)
                     /locus_tag="PK829_15030"
     CDS             complement(529..936)
                     /locus_tag="PK829_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42580.1"
                     /translation="MNENTVRWVMRADGIFNMLAGLLLQFNLTGILTALGWPPPAPET
                     RVYALVLGSALIGLSLTIWVASRRPSRAREALIISIITHLLAGLSILYEIFIAGIPLP
                     SPWLLPAAVGVQVLFVLGQLYYLLTARPANAYS"
     gene            complement(1108..>1463)
                     /locus_tag="PK829_15035"
     CDS             complement(1108..>1463)
                     /locus_tag="PK829_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42581.1"
                     /translation="DPAQYTIVSSDGVILRAAQRRRVPTQRSEQFATTMVNERTFPSA
                     TPPAADAHNNPRLSPNEVNEWLELFGPEPETKPARPLRQPNPPTPAPPPPPKKKNKDE
                     DDDLAEWLRLFGYKE"
BASE COUNT          250 a          450 c          521 g          242 t
ORIGIN      
        1 ccattggcgg ccggcactat ggcgtctatg gtcacgactg gcgggtgacg ccgccgctgg
       61 cctggctgga gctattggcc gagcgggaat tgagcgtcgg tagccggccg gcggcgcgcc
      121 cggcagccga gccgatcatc gtcctcagcg aggccgagtt tgccacagcg gtacgcgacg
      181 ccctgcgcga ctacaccgac acggcggcgc tgctggccaa tccgctgctg cgctcgcggc
      241 tggtgctggg ccaggcgggc acagacacga ctgcggcggc gcgcatcgcc gcgttgcgca
      301 agctgttgcg cgaggcggcc gagagcttgc aagactcgcc caagctggtc aagttccacc
      361 gcgcccttta ccacacctac ctgcaaccgg cggccaccca ggaacaggcc gcggaggtgc
      421 tcgatctccc gttcagcact taccgccgcc acctgcgctc cggcatcgag gaagtcactg
      481 ggcggctgtg gctgcgggag atcggcaacc tggaagccta gccgctggct aactgtaggc
      541 gttggccggc cgggcggtca acaggtaata gagctggccc aggacgaaaa gcacctgcac
      601 gcccacggcc gccggcaaga gccacggcga cggcaggggg atgccggcga tgaagatttc
      661 atagagaatc gacagcccgg ccagcaggtg ggtgatgatg ctaatgatga gcgcttcgcg
      721 ggcgcgcgac ggccggcgcg acgccaccca gatcgtcagc gacagcccaa tgagcgctga
      781 gccgagcacc agcgcataga cgcgcgtctc tggcgctggc ggtggccagc ccagggccgt
      841 caggatgccc gtcaggttga actgtaacaa caaaccagcc agcatattga agatgccatc
      901 ggcgcgcatg acccaacgga cggtgttctc attcatgaag gacctcctgc aaaacgattg
      961 tgatgtctcc aactgagtga gagcatacgc cccgccggcg ctcaattgac aggccaaaag
     1021 gtgttcattg gcaggtcaga tgggggagta ggggagtgga cagtgcccag ttggccagtg
     1081 acctctgagg tcgggcgtcg ctgggcacta ctctttgtag ccaaagagcc gcagccactc
     1141 ggccagatcg tcgtcttcgt ccttgttctt cttcttcggc gggggtgggg gcgcgggggt
     1201 gggcgggttg ggttggcgca aggggcgggc gggctttgtc tccggctccg ggccgaagag
     1261 ttccagccac tcgttgacct cgttggggct gaggcggggg ttgttgtggg catcggccgc
     1321 tggcggcgtc gctgagggga aggtgcgctc gttgaccatg gtcgtggcga actgctccga
     1381 ccgctgcgtg ggcacgcgcc gccgctgggc cgcgcgcaga atgacgccat cgctgctgac
     1441 gatagtgtat tgggccggat cct
//
LOCUS       DAOSMQ010000643         1453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_88675_length_1453_cov_6.430615, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000643 DAOSMQ010000000
VERSION     DAOSMQ010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88675_length_1453_cov_6.430615"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1453)
                     /locus_tag="PK829_15040"
     CDS             complement(<1..>1453)
                     /locus_tag="PK829_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU42582.1"
                     /translation="YIGVVGQFSDMDPASLTTDFIGWEIKNNTMSGLYRFNADNQLQP
                     LLAAALPSVSEDKLEYTIPLRAGLRFPDGSEFTADDVKWSLNRAGGLGNFLVNTYLKD
                     SNGDNFADEDAVQVIDPTTVKIIVKEPTAAFPAILTAPPFFPISSECYSDAGDPSSTC
                     GGIGPYTIANWALNDRMRLRANPDWPGEPQPAFENITVKFYPDAGSMRRSLVEFRSVD
                     LAWTGLTFNDFTELSALDADGNGANDYTAWVGPSTFKSYIIFEQTTAPWDSERVRQAA
                     ALAIDREALAAVFNGARLPLLSPVPDDIPGHLATMPARDLDRARELLRQEGYTAEKKL
                     PITLWYVNDGRYSAVEETYANTIKAQLEETGVFQVEIAGAGWDEFRLQISQCAYPTYL
                     LGWPSPGQPTSYLDATSWTDFFVTNTNRVFCSNYESEEMTELVAAARGELDDGARLEA
                     YGRIQQLWAEELPTLPLTQEPRRAISLPTIDGVR"
BASE COUNT          226 a          459 c          502 g          266 t
ORIGIN      
        1 tgcgcacgcc gtcgatggtc ggcagggaga tggcccgccg cggctcttgc gtcaggggca
       61 gcgtcggcaa ctcctcagcc catagctgct ggatgcggcc gtaggcctcc agccgcgccc
      121 cgtcatccaa ctcgccccgc gccgcggcca ccaactcggt catctcctcg ctctcgtagt
      181 tagagcaaaa gacgcggttg gtgttggtga cgaagaagtc ggtccaactg gtcgcgtcga
      241 ggtagctcgt cggctgcccc ggcgacggcc agcccagcag gtaggtcggg taggcgcact
      301 gcgagatctg cagccggaac tcgtcccaac ccgcgccggc gatctcaacc tgaaagacac
      361 ccgtctcctc tagctgggcc ttgatggtgt tggcgtaggt ttcctccacg gcgctgtagc
      421 ggccgtcgtt gacgtaccac agagtgatgg gcagtttctt ctcagcggtg tagccctcct
      481 gccgcagcaa ctcgcgcgcc cggtcgaggt cgcgggcggg catcgtcgcc aggtggccgg
      541 ggatgtcgtc gggcacgggg ctgagcagcg gcagtcgcgc cccgttgaac acggccgcca
      601 gggcttcgcg gtcgatggcc aaggccgctg cctggcgcac acgctcgctg tcccacggcg
      661 cagtcgtctg ctcgaagatg atgtagctct tgaaggtcga tggccccacc caggcggtgt
      721 agtcgttcgc gccgttgccg tcggcatcca gagccgacag ctcggtgaag tcgttgaaag
      781 tcagccccgt ccaggccaga tcgaccgagc ggaactcgac cagcgagcgg cgcatggagc
      841 cggcgtcggg gtagaacttg acggtgatgt tctcgaaggc gggttgtggc tcgcccggcc
      901 aatcggggtt ggcccgcagg cgcatgcggt cgttcagtgc ccagttggcg atggtgtacg
      961 ggccaatgcc gccgcaggtg ctgctggggt cgccggcgtc gctgtagcat tcgctgctga
     1021 tggggaagaa gggcggcgcg gtcaggatag ccggaaaggc cgccgtcggc tccttgacga
     1081 tgatcttgac cgtcgtcggg tcgatgacct gcaccgcgtc ctcgtcggcg aagttgtcgc
     1141 cgttgctatc cttcagatag gtgttgacca ggaagttgcc cagcccaccg gcgcggttga
     1201 gcgaccactt gacgtcgtcg gcggtaaact ccgagccatc ggggaagcgc agcccggccc
     1261 gcaacgggat cgtgtactcc agcttatcct ctgacaccga cggcagcgcc gcggccagca
     1321 gcggctgtag ctggttgtcg gcgttaaagc ggtagaggcc gctcatggtg ttgttcttga
     1381 tctcccaacc gatgaagtcg gtcgtcaggc tggccgggtc catgtcgctg aactggccga
     1441 cgacgccgat gta
//
LOCUS       DAOSMQ010000644         1447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_89233_length_1447_cov_3.411638, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000644 DAOSMQ010000000
VERSION     DAOSMQ010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89233_length_1447_cov_3.411638"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PK829_15045"
     CDS             complement(<1..1159)
                     /locus_tag="PK829_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42583.1"
                     /translation="MARPLPDSPGPAGAPRSPAPWWLAVAATIAVSAVLVALLSTGLC
                     PWPSLCGRIEAVDVAQARLDTALPAPQGPLVVEQSFIPQHDGLTSVELLLLRHEGHPP
                     DEATFEVALLDDSGAVVAFDRTSIVRLSHNQSFTLAFPPQRASAGRRYTIRLSGSAGN
                     VVSAWGYSLDVLSSGALALAGGALEQPPTTAARDLRFSTRYALTGGEALAAAVRPLRD
                     AGRLLLAAALLLPLPGVWLLLLFRPRRWDLPAKLGAALALGIAAWPVVWLWLTLAGGH
                     WSRPSLWAAVALGWAGAGALALWRWRPLADRRRLTTEDRLQSTDHRPLAADRRPPTTA
                     HWPLPHSLLLLLLVASLALRSVAIRDLAFPPWVDASRHALITAVMVSTGRAP"
BASE COUNT          254 a          455 c          531 g          207 t
ORIGIN      
        1 cgggtgcgcg gccggtgctg accatgacgg cggtgatgag cgcgtggcgg ctggcatcga
       61 cccagggtgg aaaagccagg tcgcgtatgg cgacagagcg gagagcgagg gaggcgacta
      121 ggaggaggag gagaagagag tggggcagtg gccagtgggc cgtggttggc ggtcggcggt
      181 cggcggccag cggccggtgg tctgtggact gtagtctatc ttcagtagtc agccggcggc
      241 ggtcggcgag cggccgccag cgccagagcg ccagagcgcc agcgccggcc cagccgaggg
      301 cgacggcggc ccagagggag gggcgcgacc aatggccgcc ggccagcgtc agccatagcc
      361 agaccactgg ccaggcagcg atgcccagcg ccagggccgc gcccagtttg gccggaaggt
      421 cccagcggcg cggccggaag agcagcagca accatacgcc cggcagaggc aggagcagcg
      481 ccgccgcgag cagcaatcgt cctgcgtcgc gcagcgggcg gacggcggcg gccagcgcct
      541 cgccgccggt cagggcatag cgtgtgctga agcgcaagtc gcgcgccgcg gtcgtgggcg
      601 gctgctccag cgccccgccg gcgagcgcca acgcgccgct gctgaggaca tcgagcgagt
      661 agccccaggc ggagacgacg ttgccggcgc tgccgctgag ccggatggta tagcggcggc
      721 cggcggaggc gcgttgcggc gggaaggcca gcgtgaagct ctggttgtgg ctcagccgga
      781 cgatggatgt tctgtcgaag gccacgaccg cgccgctgtc gtccagcagc gctacctcga
      841 aagtggcttc atccgggggg tggccctcgt ggcgcagcag cagcagttcg accgaggtca
      901 ggccgtcgtg ctgggggatg aagctctgct cgacgaccag cggcccctga ggcgcgggca
      961 gggccgtgtc cagccgcgcc tgggcgacat cgacggcttc gatccggcca cacagcgacg
     1021 gccacgggca gaggccggtg ctcagcagcg cgacaagcac agcgctcacg gcgatcgtgg
     1081 cggcaactgc cagccaccac ggggcaggcg agcgcggcgc gccggccggg ccaggcgagt
     1141 cagggagagg tcgagccatt gcgaccccaa tagtacctaa tcacgggcaa aaggaggatc
     1201 aattggttaa attggcaatc gcgtagaagt cgtaatattc attgtaagca agtcacccta
     1261 cactaaatct tgacggactt ccctccttgc aagtggcgtc acgagcgatt ttgtccctcc
     1321 ttccttctcc ccctgaagcg tgacgccact tctctttttt tccagacgcc ctgcaccgct
     1381 gatgatgcgg ttaggtaggg cctccgttca ttcccttgtc tgttaaatat cttaccctca
     1441 accccga
//
LOCUS       DAOSMQ010000645         1437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_90105_length_1437_cov_4.847323, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000645 DAOSMQ010000000
VERSION     DAOSMQ010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90105_length_1437_cov_4.847323"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PK829_15050"
     CDS             complement(<1..1421)
                     /locus_tag="PK829_15050"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU42584.1"
                     /translation="MIAVGEETRYVGDVLAEVVAATEAAARAAVALIAVEYEVLEPVT
                     DMHRALEPDAPLIHAGGNLLSRTAFVRGDMDAARAKTAYTATGIFQTQRIEHGYMEPE
                     CAIAEPLRQPRPTDAPTEPPSEADLEEVFADGQADDVPETALPVIRVLSQSQGVYDDQ
                     VQIASLLGLPLEAVRVILVPNGGGFGGKEDMSVQGHAALMAYLTGRPVKVGLTRDESI
                     IMHPKRHPIWMDYTVGCDAAGKLTYVKVKFVGDTGAYASVGMKVLERSAGHATGAYQV
                     PAADVEALAAYTNNIPCGAMRGFGVNQAIFGLESCLDDLCRQGGFDRWQFRYDNALQN
                     GDMTSTGQVIEGGAGVRETLLAVRDQFYAAKYAGLACGIKNTGVGNGMPDASHVRITV
                     VSADRVIIDHGWTEMGQGVHTIAVQTLCQETGIDPRIVRVRVDTASRQEAGMTTASRA
                     TSLVGNAVINAAAKLREDLKTHTL"
BASE COUNT          223 a          475 c          496 g          243 t
ORIGIN      
        1 agcgtgtggg tcttgaggtc ttcgcgcagc ttggccgcgg cgttgatgac ggcgttgccg
       61 accagcgacg tggcccgcga ggcggtggtc atgcccgcct cctgccgcga ggccgtgtcc
      121 acgcgcacgc ggacgatgcg cgggtcgatg cccgtctcct ggcagagcgt ctgcacggca
      181 atggtgtgga cgccctggcc catttccgtc cagccgtggt cgatgatcac ccggtcggcc
      241 gacacaacgg taatacggac gtgggaggcg tcgggcatgc cgttgccgac gccagtgttc
      301 ttaatgccac aggcaaggcc ggcgtatttg gcggcgtaga actggtcgcg cacggccagc
      361 agcgtctcgc gcacgcccgc cccgccttcg atgacctgcc cggtagaggt catgtcgccg
      421 ttctgaaggg cgttgtcata gcggaactgc cagcggtcga agccgccctg ccggcagagg
      481 tcatcaaggc agctttccag cccgaagatg gcctgattga cgccaaaacc gcgcatggcc
      541 ccgcagggga tattgttggt gtaggccgcc agcgcctcga catcggcagc gggcacctgg
      601 taggccccgg tagcgtggcc ggccgagcgc tccagcacct tcatgcccac ggaggcatac
      661 gcgccggtgt caccaacgaa cttcaccttg acgtaggtca gcttgcccgc ggcgtcgcag
      721 cccacggtgt agtccatcca gatgggatgg cgcttggggt gcatgataat agactcatcg
      781 cgcgtcaggc cgaccttgac cgggcggccg gtcagatagg ccatcagggc cgcgtgcccc
      841 tgcaccgaca tgtcttcctt gccaccaaag ccgccgccgt tgggcacaag gatcacgcgc
      901 acggcttcca gtggcaggcc cagcaacgag gcaatctgca cctggtcgtc atagacgccc
      961 tgactctggg aaaggacgcg gatgactggc aaagccgtct ctggcacgtc gtcggcctgc
     1021 ccgtcggcga agacctcctc caagtcggct tcgctgggcg gctcggttgg ggcgtcggtc
     1081 gggcgcggct ggcgcagcgg ctcggcgatg gcgcactccg gctccatgta gccgtgctca
     1141 atgcgttggg tctggaagat gcccgtggcc gtgtaggcgg ttttggcccg cgccgcgtcc
     1201 atgtcgccac ggacaaaagc ggtgcgcgag aggagattgc caccggcgtg gatgagcggc
     1261 gcgtctggct ccagggcgcg gtgcatgtcg gtcactggct ccagcacctc gtactcgacc
     1321 gcgatgaggg ccacggcggc gcgagcggcg gcttccgtcg cggcgaccac ctcggccagg
     1381 acgtcaccga cgtagcgcgt ctcctcgccg acggcgatca tcagcggcca gtccttg
//
LOCUS       DAOSMQ010000646         1424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_91294_length_1424_cov_4.811541, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000646 DAOSMQ010000000
VERSION     DAOSMQ010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91294_length_1424_cov_4.811541"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="PK829_15055"
     CDS             complement(<1..105)
                     /locus_tag="PK829_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42585.1"
                     /translation="MSEYPLEKHYLTANVIRGLAMDAVQRAESGHPGLP"
     gene            complement(184..1086)
                     /locus_tag="PK829_15060"
     CDS             complement(184..1086)
                     /locus_tag="PK829_15060"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU42586.1"
                     /translation="MSAAPTAVERATRPRLGLVLGGGGARGLAHIGVLRVLERENIPI
                     ACIAGTSMGGLIGALYAADVPIDRVEAEVVRLSRVTEQMRLVDFELSAAGLSVRGRRI
                     YNMLAELLGEGLTFADLRLPLAMAAVDIRTGRSVTLQGGLVIDAVRATISIPGIFTPV
                     DMGDYRLVDGGVLDNVPVDVAHALGATHTVAVDVLPSFSRNRPGVAPVETGLQLAFAP
                     QQFAEVYHVLMIMIAAVTEAQLRQFPPDLLIRPVLSPHVTLLSGFNRADELIAAGETA
                     AAAALPAIRALLAGEMEHETSDAT"
     gene            complement(1083..1379)
                     /locus_tag="PK829_15065"
     CDS             complement(1083..1379)
                     /locus_tag="PK829_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42587.1"
                     /translation="MQRWEYLVRVFELNKDDGVVVDYVTQMYPDRSWKELPEYDPLTL
                     EAWLNQAGQEGWELVKVEGVSAQGKRGDVGVAYPPSYPGWFHHYLCVFKRLRSE"
BASE COUNT          227 a          496 c          478 g          223 t
ORIGIN      
        1 cggcaggccg ggatggccgc tctccgcccg ctggacggca tccatggcca gcccgcggat
       61 cacgttggcc gtcagataat gcttctcaag aggatactcg ctcatatttg gctcctttgg
      121 tctgggcagc tacacgcgcc cctggtgatt gccccggcgg cgcgccggcc gcgagggcgc
      181 gggttacgtc gcgtcgctgg tttcgtgttc catctcgccc gccagcaggg cgcgaatcgc
      241 cggcagggcc gccgcggcgg ccgtctcgcc ggcggcgatg agttcgtcgg cccggttgaa
      301 gcccgacagc agcgtcacgt ggggcgacaa gaccggccgg atgagcagat cgggcgggaa
      361 ctggcgcaac tgggcctcgg tgacggcggc gatcatgatc atcagcacgt gatagacctc
      421 ggcgaactgc tgcggcgcga aggccagttg caaccctgtc tccaccgggg ccacgccggg
      481 ccggttgcgg ctaaagctgg gcaggacatc gacggcgaca gtgtgggtcg cccccagcgc
      541 gtgggccacg tcgacgggca cattgtcgag cacgccaccg tccaccagcc ggtagtcgcc
      601 catgtccacg ggcgtgaaga tgccggggat ggagatggtc gcccggacgg cgtcgatgac
      661 cagcccgccc tgcagggtga cagaccggcc ggtgcggatg tccacggcgg ccatggccag
      721 cggcagccgc aggtcggcga aggtcagccc ctcgcccagt agctcggcca gcatgttgta
      781 gatgcgccgg ccgcgcacgc tcagcccggc ggccgacagc tcgaaatcga ccagccgcat
      841 ttgctccgtc acccggctca ggcgcacaac ctccgcttcg acccggtcga tgggtacgtc
      901 ggcggcgtag agcgcgccga tcagcccgcc catgctcgtg ccggcgatgc aggcgatggg
      961 aatgttctcc cgctccagca cgcgcagcac gccgatgtgg gccagcccgc gcgccccgcc
     1021 gccgcccagc accaggccca ggcgcggccg tgtcgcccgc tcgacggctg ttggcgcggc
     1081 gctcactcgc tcctcagtcg cttgaagacg catagatagt ggtggaacca gcccgggtag
     1141 gagggcggat aggccacgcc aacgtcccct ctcttgcctt gcgcgctgac gccctcgacc
     1201 ttgaccagct cccaaccctc ctgaccggcc tggttcagcc acgcctccag cgtcaggggg
     1261 tcatattcgg gcagctcctt ccagctacgg tcggggtaca tctgggtgac gtaatcgacg
     1321 acgaccccat cgtccttgtt gagttcaaag acgcgcacca ggtattccca gcgttgcata
     1381 agacctcctc ggcaggactg tagggtatct caaaatgggg cgaa
//
LOCUS       DAOSMQ010000647         1424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_91297_length_1424_cov_4.474799, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000647 DAOSMQ010000000
VERSION     DAOSMQ010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91297_length_1424_cov_4.474799"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1424)
                     /locus_tag="PK829_15070"
     CDS             complement(<1..>1424)
                     /locus_tag="PK829_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOU42588.1"
                     /translation="LAIDFASPVRAAAAGQAARPLFGVPQAIPGGPHLRKAPCQFGWD
                     WGPQLPPIGVWKDIRLEGADGARLTDVALRGVLKDARGGPAEVSAAVSVEQWATAPLM
                     AQLRVVAPDGEEWLAEAPIAAGNATLRVVIPQPQLWWPNGYGAQPLYCAVVTLRAGDA
                     VRDERAYQLGLRHIALRQDEDQWGRSFAFWVNGVPVFAKGSNWIPADSFPTRLTADAL
                     ETLIRAAAETHQNMLRVWGGGFYEDERFYDLCDRYGILVWQDFVFACSIYPLDEPAFL
                     DTVRAEVEQNIARLRHRASLALWCGNNEIEWGWADWGLDRPENAPLKAAYDRFFHHTL
                     PQWCAAADPDTPYWPSSPSSDTPFQNPNGQRQGDAHYWDVWHGRKPFTAYRDQYPRFM
                     SEFGFQALPPLATIRTYADEVDWNMTAYVMEQHQKNASGNSLMVGQMLDTFRLPEGFD
                     ALVYLSLVLQAEGIRYGVEHWRRHR"
BASE COUNT          218 a          468 c          527 g          211 t
ORIGIN      
        1 cggtggcgtc gccagtgctc cacgccatag cggatgccct ccgcctgcaa caccaggctc
       61 aggtagacca gcgcgtcgaa gccctccggc agccggaagg tatccagcat ctgcccgacc
      121 atgaggctgt taccgctggc gttcttctga tgctgctcca tgacgtaggc ggtcatgttc
      181 cagtcgacct cgtcggcata ggtgcggatg gtcgccagcg gcggcaatgc ctggaagccg
      241 aactcggaca tgaagcgcgg atactggtcg cgataggcgg tgaagggctt gcggccgtgc
      301 cagacgtccc agtagtgggc gtcgccctgg cgctggccgt tggggttctg gaagggcgtg
      361 tccgacgagg gcgaactggg ccagtagggc gtgtccgggt cggccgcggc gcaccactgc
      421 ggcagggtgt ggtggaagaa gcggtcatag gcggctttca gcggggcgtt ttccggccga
      481 tccaggcccc aatcggccca gccccactcg atctcgttgt tgccgcacca cagcgccagg
      541 ctggcccggt ggcgcagccg ggcgatgttc tgctcgacct cggcgcgaac ggtgtccagg
      601 aaagccggct cgtccagcgg gtagatgctg cacgcgaaga cgaagtcctg ccagacgaga
      661 atgccgtagc ggtcgcagag gtcgtagaag cgctcgtcct cgtagaagcc gccgccccag
      721 acgcggagca tgttctggtg ggtctctgcc gcggcgcgga tgagcgtctc cagcgcgtcg
      781 gcggtgaggc gggtggggaa agagtcggct ggaatccagt tcgatccttt ggcaaagacg
      841 ggcacgccgt tgacccagaa ggcgaaggag cggccccatt ggtcctcgtc ctgacgcagg
      901 gcgatgtggc gcaggccgag ctggtaggcg cgctcgtcgc gcacggcgtc gccagcgcgc
      961 agagtgacca cggcgcagta gagcggctgc gcgccatagc cgttgggcca ccacagttgc
     1021 ggctgcggga tgaccacgcg cagggtggcg ttgccggcgg cgatgggcgc ttcggccagc
     1081 cactcctcgc catcgggcgc gacgacgcgc aattgcgcca tcagcggcgc ggtggcccat
     1141 tgctcgaccg acaccgccgc cgacacctca gccggcccgc cgcgggcatc cttcaggacg
     1201 ccgcgcaggg cgacgtcggt caggcgcgcc ccgtcggccc cttccaggcg gatgtccttc
     1261 cagacgccga tgggcggcag ctgcggcccc cagtcccagc cgaactggca gggcgcctta
     1321 cgcaggtgcg gcccgccggg gatggcctgt ggcacgccaa agagcggccg cgcggcctgc
     1381 ccggcggcgg cggcgcgcac gggcgaggcg aagtcgatgg ctag
//
LOCUS       DAOSMQ010000648         1421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_91652_length_1421_cov_1.963397, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000648 DAOSMQ010000000
VERSION     DAOSMQ010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91652_length_1421_cov_1.963397"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PK829_15075"
     CDS             complement(<1..805)
                     /locus_tag="PK829_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42589.1"
                     /translation="MKTLACLAVVILLAGCGASAAPAGFSAPPPTVPGMDAAAARATV
                     DAWMVIQAATAEAQALQTREAAYVTRVHEEQAQATAGAATATSQALAVSATAIAQQMM
                     VDAATADAAGMLTATSVAGTATAAPATSTAVYQLTVDAQADANVRLVQEDAATRMELE
                     QRRAETSLRWLPVLYGVGIFALLALGVAGLAFVWFLIHRARYTLLNPVVELPNGGALA
                     RLPDGRGGFGAPQLATITVPARPSSAPLPALPAPTPQPTPLPSLAGAHVL"
     gene            complement(814..1209)
                     /locus_tag="PK829_15080"
     CDS             complement(814..1209)
                     /locus_tag="PK829_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42590.1"
                     /translation="MRRGELGATLLVLALGLGAVLVAGARSAAGRMLDLAEYRARSPY
                     YQAAALRAQMAGDDSSSSAWVFVALAVIVLIVIALVALRPTLREFRLMRRRSHRPRRQ
                     PVASPTLPALPRLPAPRLPATGDETTPEH"
     gene            complement(1196..>1421)
                     /locus_tag="PK829_15085"
     CDS             complement(1196..>1421)
                     /locus_tag="PK829_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42591.1"
                     /translation="AFLAERLDPGLPPPPAAEIAAVRAAQWHMLLGFFLFVAACVALF
                     FVAMFRPDVPPSTDIDPLVEAEELVCYAKG"
BASE COUNT          239 a          481 c          521 g          180 t
ORIGIN      
        1 ttagcacgtg cgcgccggcc aaagagggca ggggggtggg ttgcggcgtg ggggcgggca
       61 atgccggcaa gggggcactc gacggccggg ccggaacagt aatggtcgcc agctgcggcg
      121 cgccgaagcc gccgcggccg tcaggcaggc gagccagcgc gccgccgttg ggcagctcca
      181 caacggggtt gagcagtgtg tagcgcgccc ggtggatgag gaaccacacg aaggccagcc
      241 cggcaacgcc cagcgccagc agggcgaaga tgccgacgcc gtagagcacc ggcagccagc
      301 gcaaggaggt ctctgcccgg cgctgctcca gttccatgcg cgtcgccgcg tcctcttgaa
      361 ccaggcgcac gttggcatcg gcctgggcgt caactgtcag ctggtacacg gccgttgacg
      421 tcgccggcgc ggccgtggcg gttccggcga cactggttgc cgtcagcatc ccggcggcgt
      481 cggcggtggc agcgtccacc atcatctgct gggcgatcgc cgtggcgctc actgccagcg
      541 cctgtgacgt ggccgtggcc gccccggctg tggcctgagc ctgctcctca tggacgcgcg
      601 tgacgtaggc ggcctcccgc gtctggagtg cttgcgcttc ggccgtcgcc gcctgaatga
      661 ccatccaggc gtccaccgtc gcccgcgccg cggcggcgtc catgcccgga acagtgggcg
      721 gcggcgcgct aaaccccgcc ggcgcagccg acgcgccgca gccggccagg agtatgacca
      781 ctgccaaaca cgccagtgtc ttcatgacga cctctagtgt tctggagtag tctcgtcgcc
      841 ggtcgccggc agccgtggcg caggcaaccg gggtagggcg ggcaacgtcg gcgacgcgac
      901 cggttgacgg cgcggtcggt gcgaacggcg gcgcatgagg cggaactcgc ggagggtcgg
      961 ccgcagagcc actagggcaa tgacaatcag gactatcacc gccagggcga cgaagaccca
     1021 ggcgctgctg gaactgtcgt cgccggccat ctgcgcccgc agggccgccg cctggtagta
     1081 gggggagcgc gcccggtact cggccaggtc gagcatccgg ccagcggccg agcgcgcacc
     1141 ggcgaccaga accgcaccta atcccagggc cagtaccagt aaggttgcgc ctagttcacc
     1201 ccttcgcata gcaaacaagc tcctccgcct ccaccaatgg gtcgatgtca gtggatggtg
     1261 gcacgtcagg ccggaacatg gcgacgaaga acagggcgac gcaggcggcg acaaacagaa
     1321 agaagcccag cagcatatgc cactgagcgg cccgaacggc ggcgatctcg gccgccgggg
     1381 gcggggggag cccaggatcc aagcgctcgg cgaggaaggc c
//
LOCUS       DAOSMQ010000649         1411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_92575_length_1411_cov_4.103245, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000649 DAOSMQ010000000
VERSION     DAOSMQ010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92575_length_1411_cov_4.103245"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1411
                     /locus_tag="PK829_15090"
     CDS             <1..>1411
                     /locus_tag="PK829_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42592.1"
                     /translation="PLFSYLVALAYRVTGPGAYGIHLASALAGIATIPAVWLAARALF
                     AGSRRGWLAWLPLLAAYLSAVSYWHLNWSRVGLRVILVPLFAALIVWALWRGFGAAEA
                     RPAAWAGGGRGYLLAGALLGLSLYTYQAARLLPVLVAVAFGLRALQRRRWTRADTVAV
                     LLVAAAALVVALPLGVYAWQHPGALSVRIQQATVLQADLPPGQQLGQLARQATTALLT
                     YSLRGDTDPQFTIPGRPSLNPFLSVGLVIGIVVALWRFRRSPYLFLLAWLALMTAPAM
                     VADQAATAKRYLGAFPAAMMLVSVGLLLPLEWLATTADRRPPTAETQTSPAELGSPTE
                     SNTPSFGNGAQLPSGGGRSSAVGRRLLSAALILGLLYTAVATWRDYFVVWAADPDLPA
                     HFQADYRAIGEAIGALDAAQPVWLSPFPADHPVIQLHAGLRPDLRAYNGRFCLPYADP
                     VGDRGTVYVIVPGLQDHSLA"
BASE COUNT          147 a          552 c          472 g          240 t
ORIGIN      
        1 tccgctgttc agctacctcg tcgccctggc ctatcgcgtc accggccccg gcgcgtatgg
       61 catccacctg gccagcgcgc tggccggcat cgccacgatc ccggcggtgt ggctggcggc
      121 gcgggcgcta ttcgccggat cgcgccgggg ctggctggcc tggctgccgc tgctggccgc
      181 ctacctgtcg gccgtgtcct actggcatct gaactggagc cgcgtcggcc tgcgggtcat
      241 ccttgtgccg ctgttcgccg cgctgatcgt ctgggcgctg tggcgtggct ttggggcggc
      301 cgaggcgcgg ccggcggcgt gggcgggtgg cggtcgtggt tacctgctgg ccggggcgtt
      361 gctcggcctc agcctctaca cctatcaggc ggcgcggctg ctgcccgtgc tggtcgccgt
      421 ggcctttggg ctgcgcgccc tccagcggcg gcgctggacg cgggcggaca cggtcgcggt
      481 gctgctggtg gccgccgcgg cgctggtcgt ggccctgccg ctgggcgtct acgcctggca
      541 acaccccggc gcgctctccg tccgcatcca gcaggccact gtcctgcaag ccgacctgcc
      601 gccgggccaa caactcggcc agctcgcccg gcaggcgacg acggcgctgc tgacctacag
      661 cctccgcggc gacaccgacc cgcaattcac catccccggc cggccgtcgc tcaacccgtt
      721 cctctccgtg ggtctggtca tcggcatcgt cgtcgccctg tggcgctttc ggcgctcgcc
      781 ctatctgttc ctgctggcct ggctggcgct gatgaccgcc ccggcgatgg tggccgacca
      841 ggcggcgacg gccaagcgct acctgggcgc gttcccggcg gcgatgatgc tggtctcggt
      901 gggactgcta ctgccgttgg agtggctggc gacgaccgcc gaccgccgac cgccgaccgc
      961 cgaaacacaa acgtcgccgg cagaactcgg ctcgccaacg gagagcaata ccccatcttt
     1021 cggcaacggc gcgcaacttc cttctggcgg cggtcggtcg tccgccgtcg gtcgtcgttt
     1081 actgtcggcc gcgctcatcc tcggcctcct ctacaccgcc gtcgccacgt ggcgcgacta
     1141 cttcgtcgtc tgggcggccg accccgacct gccggcccac ttccaggccg actatcgcgc
     1201 catcggcgag gccatcggcg cgctggacgc ggcgcaaccg gtctggctgt cccccttccc
     1261 ggccgaccac cccgtcatcc aactccacgc cggcctgcgc cccgacctgc gggcctacaa
     1321 cggccgcttc tgcctgccct acgccgaccc cgtgggcgac cgcggcacgg tctacgtcat
     1381 cgtgcccggc ctgcaagacc acagcctggc c
//
LOCUS       DAOSMQ010000650         1409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_92747_length_1409_cov_5.697194, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000650 DAOSMQ010000000
VERSION     DAOSMQ010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92747_length_1409_cov_5.697194"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PK829_15095"
     CDS             complement(<1..603)
                     /locus_tag="PK829_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU42593.1"
                     /translation="MIRIPRHLSQFLLLAVLLSLAACRLSAAETASTATAAPPPTPIV
                     VVATPTPLSDSDLLPIDIEEQLITNLYERVGPSVVHISSEVVTMDFFFGPMSSEGTGS
                     GFVWDAAGHIVTNYHVVADANKIEVIFSDNRQVEAEVVGVDPRNDLAVLRVDPGTVAV
                     PPLPVAAADDVRVGQRAIAIGNPFGLDRTLTTGVVSALGRP"
     gene            complement(627..>1409)
                     /locus_tag="PK829_15100"
     CDS             complement(627..>1409)
                     /locus_tag="PK829_15100"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HOU42594.1"
                     /translation="DHLVTTGAPQSNHCRQTAAAAARFGLGCSVVLRGRKPEATTGNL
                     LLDHLLGAHVYWSDAGECGEAVAAVLAELRSLGRRPYLIPLGGSNVVGATGYVLAMRE
                     LMAQLEAERLNVDFIVFASSSGGTQAGISLGAHVYGYGGTVLGISVDHPADALRTQVA
                     ALTTATAAHVGLDGVSVAERISVNDDYLGEGYAHVGETEREAIRLVAQLEGILLDPVY
                     TGRAMGGLIDLIRWGAFTRGQRVLFWHTGGTAALPAFAERLL"
BASE COUNT          247 a          473 c          491 g          198 t
ORIGIN      
        1 cggccggccc agcgcgctga ccacacccgt cgtcagcgtc cggtcaaggc caaacgggtt
       61 gccgatggcg atggcccgct ggccaacgcg cacatcgtcg gccgcggcca ccggcagcgg
      121 cggcacagcg accgtacccg gatcaacgcg cagcacggcc agatcgttgc gcggatcgac
      181 gccgacgacc tcggcctcca cctggcggtt gtcggagaaa atgacctcga ttttgttggc
      241 gtcggcgacg acatggtagt tggtgacgat gtggccggcg gcgtcccaga cgaagccgga
      301 gccggtgccc tcgctcgaca tgggaccgaa gaagaagtcc atcgtcacca cttccgagct
      361 gatgtgcacc actgacggcc caacgcgctc ataaaggttg gtgatcagct gctcctcgat
      421 gtcgatgggc agcaggtcgc tgtcggacag gggcgtgggc gtggccacaa cgacaatggg
      481 ggtcggcggc ggggcggcgg tagccgtcga cgcagtctcg gccgccgaca ggcggcaggc
      541 agccagcgat aacaacaccg ccagcagtaa gaactggctc agatgacggg ggatacgaat
      601 catagacgcc tcgccaggca gagtcgctag agtagccgct cggcaaaggc cggcagcgcc
      661 gccgtgccgc ccgtgtgcca gaacagcacg cgttggccac gggtgaaggc tccccagcga
      721 atgagatcga tcagaccgcc catggcccgg ccggtgtaga ccgggtcgag gagaatccct
      781 tccagttggg ctaccagccg gatggcctcg cgctctgtct cgccaacgtg ggcgtagccc
      841 tcacccaggt agtcgtcgtt gacgctgatg cgctcggcca cggagacacc atcaaggccg
      901 acgtgcgcag ctgtggccgt ggtcagggcg gccacctgcg tacgcagggc gtcggcggga
      961 tgatcgacgc tgatgcccag caccgtcccg ccatagccgt agacgtgcgc ccccagggag
     1021 atgcccgcct gcgtgccgcc gctgctgctg gcgaagacga tgaaatcgac attgagacgc
     1081 tccgcttcca gttgggccat cagttcgcgc atggccagca cgtagccggt cgcgccgacg
     1141 acgttggagc cgcccagggg gatcaggtag ggccgccggc caagcgagcg caactcggcc
     1201 agcaccgcgg cgacggcctc accacattcg ccggcgtcgc tccaatagac gtgcgcgccc
     1261 agcaggtggt cgagcagcag gttgcccgtc gtggcctccg gcttgcggcc gcgcaggacg
     1321 acactgcacc ccaggccaaa gcgcgccgcg gcggcggccg tctgccggca gtggttgctc
     1381 tggggcgcgc cggtggtcac caggtggtc
//
LOCUS       DAOSMQ010000651         1409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_92790_length_1409_cov_2.628508, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000651 DAOSMQ010000000
VERSION     DAOSMQ010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92790_length_1409_cov_2.628508"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PK829_15105"
     CDS             complement(<1..508)
                     /locus_tag="PK829_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU42595.1"
                     /translation="MAGLSLHERLEQARRRQFVGRVGERDRLRAVLGEPELPFFVLHI
                     FGPGGLGKTSLLREYGYIARELGIQSVLLDGRNFDASPVGFLGALGYALHLPAGGDPA
                     ATLAERGERLVLLVDTYELLNPIDNWLREEFLPGLSGNVFVVLAGRQPPAAAWRIDPG
                     WQTMLRVLP"
     gene            680..>1409
                     /locus_tag="PK829_15110"
     CDS             680..>1409
                     /locus_tag="PK829_15110"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOU42596.1"
                     /translation="MTMAAPLSAAVPGKTRRIRLGGITALAQSAWMGFPLAADVLEPI
                     RSAPAALRRWTEIIRPRRPAGGLRGLFNREFGIAEASFILMVSFFTSAALGAVRQVLF
                     NAQFGVSAEANAYYAAFRLPDTLFSLIAGGALSSAMIPVLLSARQNDGERAAWRLVSL
                     VLTALLAFMALVVLIVGLFTPFFVRTLLAPGFDATTSQLTVTLTRVMLVQPLVLAVGS
                     VATAVLNSRNRFLPTALSIVSHNVA"
BASE COUNT          223 a          515 c          441 g          230 t
ORIGIN      
        1 ggggcagcac acgcagcatc gtttgccagc cggggtcgat acgccacgcc gccgccggtg
       61 gctgtcgccc ggccaggaca acgaagacgt tgcccgacag accgggcaaa aactcctcgc
      121 gcagccagtt gtcgatgggg ttgagcagct cgtaggtatc gaccagcagg actaatcgct
      181 ccccgcgctc ggccagggtg gccgccgggt cgccgccggc cggcaggtgg agagcatagc
      241 ccagcgcgcc caggaaaccc accggcgaag catcgaagtt gcgcccatcg aggaggacgc
      301 tctggatgcc cagctcgcgg gcgatgtagc cgtactcgcg cagcaggctg gtcttgccca
      361 gcccgcccgg cccgaaaatg tgcagaacga agaagggcag ctctggctcg cccagaaccg
      421 ccctcaggcg gtcacgttcg cccacgcggc cgacgaactg ccgccggcgc gcctgctcca
      481 accgttcatg aagggatagg ccagccacca tccactcctc ggcccccata gccctttttg
      541 accagacaga ggaattgtcg ccatccgccg tcaaatgtca atggccaatt gagcagcaat
      601 tggacaccgt ttggtctggc ctggccgccg ggtttgcccg tatgatgttc atgattcaga
      661 accggagaag gacctgacga tgacaatggc agcccctcta tccgcagcgg ttcccggcaa
      721 aacccgccgc atccgcctgg gcggcatcac cgccctggct cagagcgcgt ggatgggctt
      781 ccccctggcg gccgacgtgc tcgaacccat ccgctcggcc ccggcggcgc tgcgccgctg
      841 gacggagatc atccgcccgc ggcggccggc cggcggcctg cgcggcttgt tcaaccgcga
      901 gttcggcatc gccgaggcca gcttcattct catggtctcg ttcttcacct cggcggcgtt
      961 gggggcggtg cggcaggtgc tcttcaacgc tcaattcggc gtcagcgccg aggccaacgc
     1021 ctactacgcc gccttccgcc tgccggacac actcttcagc ctcatcgccg gcggggcgct
     1081 gtccagcgcc atgatccccg tgctgctgag cgcccggcag aacgacggcg agcgggccgc
     1141 ctggcggctg gtcagtctgg tgctgacggc gctgctggcc ttcatggccc tcgtcgtgct
     1201 catcgtcggt ctgttcacac ccttcttcgt gcgcacgctg ctggcccccg gcttcgacgc
     1261 cacgaccagc caactgacgg tgacgctgac gcgcgtcatg ctcgtccagc cgctggtgct
     1321 ggccgtcggc agcgtggcca cggctgtgct caacagccgc aaccgcttcc tgcccacggc
     1381 cctgtccatc gtcagccaca acgtcgccc
//
LOCUS       DAOSMQ010000652         1406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_93059_length_1406_cov_3.207254, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000652 DAOSMQ010000000
VERSION     DAOSMQ010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93059_length_1406_cov_3.207254"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK829_15115"
     CDS             complement(<1..839)
                     /locus_tag="PK829_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU42597.1"
                     /translation="MHKRARLLPLLLALAGAVALAQPLVLLAQTQPTPTAATPTLATE
                     AAATPPAAGTPATDPAASPVPRVHVVEDGENLTTIAADYGVTVEDILAINNLANGDLL
                     QVGQELIIPGGTGEAIATVYTIAAGDTLAGVAATFNTTLADVLATNRLIAADLPLIVG
                     QTVPVISRTGSASPRPVTGRPYLVTPGDTLLTVAAAHNLAPAALRAANNLTGDYLFPG
                     QRLRLPDDTTTYRHLPEGWLDVRVTPASVGQGSTVSVYVANVLPGTPAGRHVWRQGTA
                     RPRG"
     gene            complement(906..>1406)
                     /locus_tag="PK829_15120"
     CDS             complement(906..>1406)
                     /locus_tag="PK829_15120"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU42598.1"
                     /translation="VPLPENVAAHSFGVVYTTLLLADLIEGPIDLAAALALAALHDLP
                     EALTTDIPTPAWRFLPSGSKTDAERQAMTHIAGEGPVGTRWLAWWEEMRRNESAEARL
                     VHDADRLDLYLQAHIYELHTGNRQLEEFWRVAHRFFYPQAQAIYDELRRRRDPSALVT
                     EAPLRP"
BASE COUNT          208 a          448 c          538 g          212 t
ORIGIN      
        1 ccgcgcgggc gggccgtgcc ctggcgccag acgtgccggc cggcgggcgt gcccggcaag
       61 acgttggcga cgtagaccga caccgtcgag ccttgcccca ccgaggccgg cgtcacgcgc
      121 acgtcgagcc agccctccgg caggtggcga taggtggtcg tgtcgtcggg caggcgcagg
      181 cgctggccag ggaagaggta gtcgccagtc aggttgttgg ccgcgcgcag agccgcggga
      241 gccaggttgt gggccgcggc cacggtcaac agcgtgtctc cgggggtcac caggtaaggc
      301 cgccccgtca ccggccgcgg cgaggccgac cctgtccggc taatgaccgg caccgtttgg
      361 ccgacgatga gcggcaggtc ggcggcgatc aggcgattgg tggccagcac gtcggccagg
      421 gtggtgttga aggttgcggc cacaccggcc agcgtatcac ccgcggcaat ggtgtagacc
      481 gtggcaatgg cctcgcctgt tccgccgggg atgattagct cctgcccgac ttgcagcaag
      541 tcgccgttgg ccaggttgtt aatggccaga atgtcctcaa ccgtcacgcc gtagtcagcg
      601 gcgatggtgg tcagattctc gccatcctcc acaacgtgga cgcggggcac aggcgacgcg
      661 gccgggtcgg tcgcgggcgt gcccgcggcg gggggggtag cggcagcctc ggtcgccaac
      721 gtgggcgtcg ccgcagtagg cgtcggctgg gtctgcgcca gcaagaccag cggctgcgcc
      781 agggcgacgg ccccggccag agccagaagg aggggcagga ggcgagctcg tttgtgcata
      841 ggccgcggct tattctgccc gaaggcggga cagttgccaa tagggtctct aggcgcgctc
      901 agcattcagg ggcgcaatgg cgcttcggtc accaatgccg aggggtcgcg ccggcggcgc
      961 aactcgtcgt agatggcctg agcctggggg tagaagaagc ggtgggccac gcgccagaac
     1021 tcctccaact ggcggttgcc ggtgtggagt tcgtagatgt gggcttgcag gtaaaggtcg
     1081 agccggtcgg catcgtggac cagtcgggcc tcggccgatt cgttccgccg catctcttcc
     1141 caccaggcca gccagcgcgt ccctaccggc ccctccccgg cgatgtgggt catggcctgt
     1201 cgctcagcgt cggtcttgga gccgctgggc aggaagcgcc acgcgggggt gggaatgtcg
     1261 gtcgtcagcg cctccggcag gtcgtggagc gcggccaggg ccaacgccgc ggccaggtcg
     1321 atgggcccct cgatcaggtc ggccagcagc agcgtcgtgt agacgacgcc gaagctatgg
     1381 gcggcgacgt tctccggcag cggcac
//
LOCUS       DAOSMQ010000653         1388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_94873_length_1388_cov_3.485371, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000653 DAOSMQ010000000
VERSION     DAOSMQ010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94873_length_1388_cov_3.485371"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1388
                     /locus_tag="PK829_15125"
     CDS             18..>1388
                     /locus_tag="PK829_15125"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU42599.1"
                     /translation="MTNYIDYQIAEIYAANTDWPQSNVRYWRAYAPGSQWRWILHDLD
                     TAFGNNMANGVGQNTLRLAMSKKGPKAYHSLILIQLSRNADFRALFAQRFAAHLNTTF
                     APERMTGLLNNMSATIAADMPAQTARWKLPKGVGFWNTQLTKMRAFLQKRPAFVITHL
                     NEYLGKPGTALLTTAAGAAGDIVVAGVPVPDSYSGLAFKNTPLTLTAVPAPGQLFVRW
                     AETGQTTPQITITLAGAATYTAIFDAEPPPPPVPDIVINEIHYRPLPESAEPDREFIE
                     LYHAGATTADLSGYTISAISYTFPNGAQIAPGEYIVVAANSADPAYAALAPGRVFDWD
                     WGDDAGHKLSNSSEAVTLRHADGRVVDTVTYDESSPWPPEADGDGPSLALLDETLDNA
                     LPASWAKSREIGGTPGAENFPPRPPVPAVVINEIHYNPADAQGPDAGYEFIELVNTSG
                     EPVALEG"
BASE COUNT          269 a          520 c          412 g          187 t
ORIGIN      
        1 tgcagacgca gatggacatg accaactaca tcgactacca gatcgccgag atctacgccg
       61 ccaacaccga ctggccgcag agcaacgttc gttactggcg cgcctacgcg cccggcagcc
      121 agtggcggtg gattctgcac gacctcgaca cggccttcgg caacaacatg gccaatggcg
      181 tcggccagaa caccttacgg ctggccatgt ctaagaaagg gccaaaggcg tatcatagcc
      241 tcatcctgat ccagctgagc cgcaacgccg acttccgcgc cctgttcgcc cagcgctttg
      301 ccgcccacct gaacaccacg tttgcgccgg agcgcatgac cgggctgctc aacaacatgt
      361 cggccacaat tgccgcggac atgcccgcgc agaccgcccg ctggaagctg cccaagggcg
      421 tgggcttctg gaacacccag ctgaccaaaa tgcgggcctt cctgcaaaag cgccctgcct
      481 tcgtgatcac ccatctcaac gagtacctcg gcaaaccggg cacagcgctg ctgacgacag
      541 ccgccggcgc ggccggcgac atcgtcgtcg ccggcgtgcc cgtgcccgac agctacagcg
      601 gcttggcctt caagaacacg cctctgacgc tgacagccgt gcccgcgccg ggacaactgt
      661 tcgtccgctg ggccgagacg ggccagacaa cgccacagat caccatcacc ctggccggcg
      721 cggcgaccta tacggccatc ttcgacgccg agccgccgcc tccgcccgtg cccgacatcg
      781 tgatcaacga gatccactat cgcccgctgc ccgaatcggc cgaaccggac agggagttca
      841 tcgagctata ccacgccggg gcgacgacgg ccgacctgag cggctacacc atcagcgcca
      901 tcagctacac cttccccaac ggggcgcaga tcgcgccggg cgagtacatc gtcgtcgccg
      961 ccaacagcgc cgacccggcc tacgccgccc tggcccccgg ccgcgtcttt gactgggact
     1021 ggggcgacga cgccggccac aagctgagca acagcagcga ggcggtgacc ctccgccacg
     1081 ccgacggccg cgtggtcgat accgtgacct atgacgaaag ctcgccctgg ccgcccgagg
     1141 cggacggcga tgggccgtca ctggccctgc tcgacgaaac cctcgacaac gccctgcccg
     1201 ccagctgggc caaaagccgc gagattggcg gcacgcccgg cgcggagaat ttcccgccgc
     1261 ggccgcctgt cccggctgtg gtcatcaacg agatccacta caacccggcc gacgcccagg
     1321 ggccggacgc cggctatgag ttcatcgaac tggtcaacac gagtggcgag ccggtggcgc
     1381 tggaaggc
//
LOCUS       DAOSMQ010000654         1382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_95503_length_1382_cov_3.319518, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000654 DAOSMQ010000000
VERSION     DAOSMQ010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95503_length_1382_cov_3.319518"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PK829_15130"
     CDS             complement(<1..1158)
                     /locus_tag="PK829_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42600.1"
                     /translation="MASMALLALMAGAVAVAALLLVAGAVLVWLVWPPEGGAIKALAA
                     VVPASLLAGLLYVFLHAARYRSLLFYQGSTQPQTAAELRALEAGQVIGLLTTFYIFLF
                     LIMAALSVALAWPALRESRRAWSDAPRPLAFGALAVLLVGALWLSGHSNVRVIQADMI
                     YKRAKPFDDQATRATSADPALRRQTWDAAIAIYDAAVAHAPKEDFYYLFLGRALLERA
                     SLSEDAVEKAALLSRAETLLLEAQQIAPLNTDHTANLARLNSRWYAASDDPAERAERL
                     DLAVRTYETALVLSPQNTIVRNELARLVLEVVGDCDRALALYDESALIDPFYAQTQLS
                     RADAYITCGDPLPAAERAAHFRTAAEALDSGLLLSPANLRAWIQLAEIRRQL"
     gene            1148..1351
                     /locus_tag="PK829_15135"
     CDS             1148..1351
                     /locus_tag="PK829_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU42601.1"
                     /translation="MLAIATPKIPAATAAPGTIRGDLAVEIGRNLVHGSDGPESAARE
                     VALFFQEDELVSWSRETDRWIRE"
BASE COUNT          227 a          454 c          505 g          196 t
ORIGIN      
        1 cagttgccgc cggatttcgg ccagttgaat ccaggcgcgg aggttggccg ggctgagcag
       61 taggccgcta tccagcgcct cagcggcggt gcggaagtgc gcggcgcgct cggccgccgg
      121 caggggatcg ccacaggtga tgtaagcgtc ggcgcgggac agttgcgttt gggcgtagaa
      181 cgggtcgatg agggccgatt cgtcatagag ggccagagcg cggtcgcaat cgccgacgac
      241 ctccagcacc agacgggcca actcgttgcg gacgatggtg ttctgcgggc tgaggacgag
      301 cgccgtctcg taggtgcgca cggccaggtc aagccgttcg gcccgctcgg ccgggtcgtc
      361 gctggcggca taccagcgcg agttgagccg ggccagattg gccgtgtggt cggtgttgag
      421 cggcgcgatc tgttgcgcct ccagcaggag cgtctcggcg cgcgatagca gggccgcttt
      481 ctcgaccgcg tcctcgctca ggctggcccg ctccagcagc gcccggccga ggaacaggta
      541 atagaagtcc tccttggggg cgtgggccac ggcggcgtcg tagatggcga tggccgcgtc
      601 ccacgtttgg cggcggaggg ccgggtcggc cgaggtcgcc cgcgtagcct ggtcgtcgaa
      661 aggcttggcg cgcttgtaga tcatgtccgc ctggatgacg cggacgttgc tgtgcccgct
      721 cagccagagc gccccgacca gcaacacggc cagcgcgccg aaagccagtg gccggggcgc
      781 gtcgctccac gcccggcggc tctcgcgcag cgccggccag gccagggcga ccgacagcgc
      841 cgccatgatg agaaacagga agatatagaa tgtcgtcagc aggccgatca cctgcccggc
      901 ttccagggcg cgcaattcgg ccgccgtctg cggctgggtg ctgccctggt agaagagcag
      961 ggagcggtag cgcgcggcgt ggaggaagac gtagagcaga ccggccagca gcgaggcggg
     1021 cacgaccgcg gccagcgcct tgatcgcgcc cccctcgggt ggccacacca gccacaccaa
     1081 caccgcgccg gccaccagca gcagcgcggc aacagcgacg gccccggcca tcagcgccag
     1141 cagggccatg ctggccattg ccacgcccaa aatacccgcc gccacggccg cgccgggcac
     1201 cattcgcggc gacctggcgg tggagatcgg tcgcaatctg gttcatggct ccgacggccc
     1261 ggaaagcgcc gcccgtgagg tagcgctctt cttccaggaa gatgaactcg tatcctggtc
     1321 gcgggaaacc gaccggtgga ttcgcgaata agcggcgcgc gactggccgc tatgaatgtc
     1381 ta
//
LOCUS       DAOSMQ010000655         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_98202_length_1356_cov_3.110684, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000655 DAOSMQ010000000
VERSION     DAOSMQ010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98202_length_1356_cov_3.110684"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PK829_15140"
     CDS             complement(<1..646)
                     /locus_tag="PK829_15140"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU42602.1"
                     /translation="MKTLWQRRKKWIIAGAAVLVAALALLAAGPLLSARNGAGTTTAA
                     VERVEVVVGDLSSEATASGELSARQSAALALMTSGTIDAVLVAVGDEAHAGQPLLRLD
                     TTELERNVANGQQALLSREAELEDLVAPPTAADLAAAEAAVASAQASLDDLLDGPSDT
                     ELAAAEANLRAAQADLGAASARLDTAQSGASDEAVRAAQLRLESAQRAATSAAER"
     gene            complement(653..>1356)
                     /locus_tag="PK829_15145"
     CDS             complement(653..>1356)
                     /locus_tag="PK829_15145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU42603.1"
                     /translation="GPTAAQRASAEAAVEQARLALADAEDALANATITAPFDAAVTAV
                     HVQPGEIAAGPVVGLVDLGSLQVVLQVDEVDVGNMAVGQPATVTLESFPDAVIDAEVA
                     AIAPAAAMSSTGLVTYDVRLRLDDSGLPLLAGMTANATLVTAERRDVLLVPNEAISVD
                     RTTGTYSVRRQTATGAETVTIVIGLRDNQYTEVRDGLSAGDVLLIGETDSSNGAPGFG
                     DNGPFGGARRQLRGG"
BASE COUNT          183 a          484 c          471 g          218 t
ORIGIN      
        1 gccgctcggc ggccgaagtg gcggcgcgtt gggcgctctc cagccgcaat tgggcggcgc
       61 gcacggcctc atcgctcgcg ccggattgcg cggtgtcgag tcgggccgaa gccgcgccca
      121 gatccgcctg ggcggcgcgc agattggctt cagcggcggc cagttccgtg tcgctagggc
      181 cgtcgagcag gtcatcgagc gaagcctggg cgctggccac ggcggcttcg gccgcggcca
      241 ggtcggcggc cgtcggtggg gccaccaggt cttccagttc ggcctcgcgc gagagcagcg
      301 cctgttgccc attggccacg ttgcgctcca actccgtcgt atctagtcgc agcagcggct
      361 ggccggcgtg cgcctcgtcg ccaacggcga ccagcaccgc gtcgatcgtg ccgctggtca
      421 tcagggccag ggccgcgctc tggcgcgccg acagttcgcc ggaggccgtg gcctcgctcg
      481 acaggtcacc gacaactacc tcgacccgct ccacggcggc ggttgttgtg ccggccccgt
      541 tccgcgccga cagcaacggc ccggccgcca gcaacgccag cgccgcaacc agcacggccg
      601 cgccggcaat gatccacttt ttgcgtcgtt gccatagagt tttcatgatg atctagcctc
      661 cccgcagttg ccgccgcgcg ccgccgaacg ggccgttgtc gccgaaaccg ggcgcgccat
      721 tcgacgaatc cgtctcgccg atgagcagta catccccggc cgacagcccg tcgcggactt
      781 cagtgtactg gttatcgcgc aggccgatga cgatcgtcac tgtttccgcg cccgtcgccg
      841 tctgtcgccg cacgctgtag gtgccggtgg tgcgatcgac gctgatagcc tcgttgggca
      901 caagcagtac gtcgcgcctc tcggccgtca ccagcgtggc gttggcggtc ataccagcca
      961 gcaggggcag gccgctatcg tccaggcgta agcgcacgtc gtaggtcacc aggccggtgc
     1021 tggacatggc cgccgccgga gcgatggcgg ccacctcggc atcgatcacc gcgtcgggga
     1081 agctctccag cgtcactgtt gccggctgac cgacggccat gttgcccacg tccacctcat
     1141 cgacctgcaa gaccacttgc aggctgccca gatcgacgag gccaacgacc ggcccggcgg
     1201 cgatttcgcc cggctggacg tggacggcgg tgacggcggc gtcaaacggg gcggtgatgg
     1261 ttgcgttggc cagggcatct tcggcgtcgg ccagcgccag tcgggcttgc tcgacggcag
     1321 cctcggccga ggcgcgttgc gcggccgttg ggccgg
//
LOCUS       DAOSMQ010000656         1342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_99728_length_1342_cov_4.253302, whole genome shotgun sequence.
ACCESSION   DAOSMQ010000656 DAOSMQ010000000
VERSION     DAOSMQ010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99728_length_1342_cov_4.253302"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK829_15150"
     CDS             complement(<1..383)
                     /locus_tag="PK829_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42604.1"
                     /translation="MRTPYIVGPPVRLPTDFFGRAAQTRQFYDLLAGPQVQCVSVLGL
                     RRAGKTSFLQHVAHPEVMAGYLSDAGRYLMVAVDVSACKSPADFYARVGRRLLAALSP
                     RVARPEPPGDAYGLESLLYDLADRRV"
     gene            complement(380..>1342)
                     /locus_tag="PK829_15155"
     CDS             complement(380..>1342)
                     /locus_tag="PK829_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU42605.1"
                     /translation="DVSSTSDIAMSDRANNPANPYVVGRPLTGASAALFVGREEVFDW
                     LAENLAADGPPNALLLYGERRIGKTSTLYQLVEGERGRPLRAGRTRRLLPTYVDLQRL
                     AGCRTDEWLRRLARDIYRQVAQQRPTRAMPKSRAGDDSAYAVFDRALDYLEEHLPAGD
                     VILLAIDELEQMRAGIDAGLLDEAIVPFLRSQVQHRARLAVVFCGSYGLLEAAWQPLI
                     DLTARYELGPLDFAATTTLVRAPLAGELAFDEAAVERIWRQTGGRPFAIQSLCHRLVG
                     LRHRQRRRDPIRVEDVQAAIDEVAAEAHGPGRPLVGGAAVGACL"
BASE COUNT          223 a          460 c          480 g          179 t
ORIGIN      
        1 acgcgccggt cggccaggtc gtagagcagc gattccaggc cgtaagcgtc gccgggcggc
       61 tctggccggg cgacccgcgg cgacagcgcg gccagcaggc ggcgaccgac gcgggcgtag
      121 aagtcggccg gggacttgca ggcggaaaca tcgacggcga ccatcagata gcgcccggcg
      181 tcgctcagat agccggccat gacctccggg tgggcgacgt gctgcaaaaa agaggtcttg
      241 cccgcccgcc gcaaccccag gacgctgacg cactgcacct gtggcccggc cagcaggtcg
      301 taaaactggc gcgtctgggc ggcgcggccg aagaagtccg ttggcaggcg cacaggtggc
      361 ccgacgatgt agggcgtcct cacaggcacg ctcccacggc ggccccgccc accagaggcc
      421 ggcccgggcc gtgcgcctcg gccgcaacct cgtcgatggc tgcctgtacg tcctcgacgc
      481 ggatggggtc gcgccggcgc tggcggtggc gcaggcccac caaccggtgg caaagggact
      541 ggatggcaaa gggacggccg ccggtctgtc gccagatgcg ctcaacagcg gcctcgtcaa
      601 aggccagctc tccggccagc ggcgcgcgga cgagcgtcgt cgtggccgcg aagtcgagcg
      661 gccccagctc atagcgcgcg gtcagatcga ttagcggctg ccaggccgct tctagcaggc
      721 cgtagctgcc gcagaacacc accgccaggc gcgcgcgatg ctgcacctgt gagcgcagga
      781 acggcacgat ggcttcgtcg agcaggcccg cgtcgatgcc ggcgcgcatt tgctccaatt
      841 cgtcgatggc cagcaggatg acatcgccgg cgggcaggtg ctcctccaaa tagtcgaggg
      901 cgcggtcgaa gacggcgtag gccgaatcat cgccggcgcg gctcttgggc atggcccgcg
      961 tggggcgctg ttgggccacc tggcggtaga tgtcgcgggc caggcggcgc agccactcgt
     1021 cggtgcggca accggccaga cgctgcaagt ccacataggt aggcagcagc cgccgcgtcc
     1081 tgcccgcgcg caacggccgg ccgcgctcac cctcgaccaa ttggtagagc gtcgaagttt
     1141 tgccaatgcg acgctcgcca tagagcaaca gggcgttggg cggaccgtcg gcggccagat
     1201 tctccgccag ccagtcgaaa acttcctcgc ggccgacaaa cagcgccgcc gacgcgccgg
     1261 tcagcggccg gccgacgacg tagggattcg ccggattgtt ggcgcggtct gacattgcaa
     1321 tgtcgctcgt gctgctcaca tc
//
LOCUS       DAOSMQ010000657         1325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_101638_length_1325_cov_3.603937, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000657 DAOSMQ010000000
VERSION     DAOSMQ010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101638_length_1325_cov_3.603937"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PK829_15160"
     CDS             <1..648
                     /locus_tag="PK829_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42606.1"
                     /translation="AADFVALMRRGAAGVRAADPAALVISGAPATTGINDGVNAIDDR
                     VYFKAMLAAGAAEFIDGAGVHPYGWANPPDAHAADATQVSSSHNNHPSFFFADTLADY
                     RALLVDAGAAGVPLWPTEFGWGTFENIRFEDGSPAAPPAGSEFMADNSEWEKAVYTLR
                     AFELGQEWDWVGPMFLWNLNFTRALGPEFQEVGYSLLRFDDSRRPVYRALENRGE"
     gene            770..>1325
                     /locus_tag="PK829_15165"
     CDS             770..>1325
                     /locus_tag="PK829_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42607.1"
                     /translation="MDLIASATSDPTIVAAIASAIGNGLFFVGRKLVEKAVVEPAVDI
                     PAAGMKERIQRGHRKSREDQALRRAVQQAFAAIEAPDEAEALERYAIQLGFDQLRAED
                     NDALRQEFARAMLLVTAPEAELVPESLYDSLRWPRDRRPQLALFLYSLRRALATHENW
                     GPLITYTDSEVVRLQLRQATTSLAH"
BASE COUNT          217 a          443 c          454 g          211 t
ORIGIN      
        1 gcggccgact ttgtggctct catgcggcgc ggcgcggccg gtgtgcgcgc ggccgacccg
       61 gcggcgctgg tcatcagtgg cgcgccggcg acgacgggca tcaacgacgg cgtcaacgcc
      121 atcgacgacc gcgtctactt taaggcgatg ctggccgcgg gcgcggccga gttcatcgac
      181 ggcgctggcg tccaccccta cggctgggcc aacccgcccg acgcccatgc cgccgacgcg
      241 acacaggttt cctccagcca caacaaccac cccagcttct tctttgccga cacgctggcc
      301 gactaccgcg ccctgctggt cgatgccggc gcggcgggcg tgcccctctg gccgacggag
      361 ttcggctggg ggacgttcga gaatatccgc ttcgaggacg gctcgcccgc cgcgccgccg
      421 gccggctcgg agttcatggc cgacaatagc gaatgggaga aggccgtcta cacgttgcgc
      481 gccttcgagc tgggccagga gtgggactgg gtcgggccga tgttcctgtg gaacctcaac
      541 ttcacccgcg ccctggggcc ggagttccag gaggtcggct acagtctgct gcgcttcgac
      601 gatagccggc ggccggtcta tcgggcgctg gagaatcggg gggagtgacg cgccgccaga
      661 gaagcaccat gctctggttc atacacagca tggcgctcat tgacggaggt ttagagggtg
      721 cccgcggttg cgctatacta acgtagagca gtctgtggga gtgtgccata tggacctgat
      781 tgcctccgcc acgtcggatc caaccatcgt agccgctatt gcaagcgcaa ttggcaacgg
      841 cctcttcttc gtcggccgca agctggtcga gaaggcagtc gtcgaaccgg cggttgacat
      901 tccagccgcc gggatgaagg agcggatcca gcgcggccac cgcaagagcc gcgaagatca
      961 ggccctgcgc cgggccgtgc aacaggcgtt tgcggcgatt gaagcgccgg acgaggcgga
     1021 ggcgctggaa cgctacgcca tccagttggg cttcgaccag ttgcgggccg aggacaacga
     1081 cgccctgcgg caggagttcg cccgggcaat gctgctggtc accgcgcccg aggctgagtt
     1141 ggttccggag agcctctacg acagcctgcg ctggccgcgc gaccggcggc cgcaactggc
     1201 gctcttcctc tacagcctgc gccgggcgct ggcgacgcac gagaactggg gcccgctgat
     1261 cacctacacc gatagcgaag ttgtccgcct gcaattgcgc caggcgacga ccagcctggc
     1321 ccaca
//
LOCUS       DAOSMQ010000658         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_102492_length_1317_cov_5.073693, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000658 DAOSMQ010000000
VERSION     DAOSMQ010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102492_length_1317_cov_5.073693"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1228
                     /locus_tag="PK829_15170"
     CDS             119..1228
                     /locus_tag="PK829_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOU42608.1"
                     /translation="MSSTYLEIQLIAVFVGVACALPGVFLVLRRMAMMSDAISHTVLL
                     GIVLGFFAIGSLESPLLVLAAAAMGVLTVSLVEVLMRTHLVRQDAAIGLVFPALFSIA
                     VILISRFARGVHLDVDAVLLGELAFAPLDRMNLFGLDLPRSLVVMGVIMLANAALIAV
                     FYKELKLTTFDAGLAAALGFSPALAHYGLMFMVSVTAVGAFDAVGSILVVALMIAPPA
                     AAYLLTERLSRMLLYSAALATAGAISGFWLAVALDANVAGAIATTLGALFLGVFLLAP
                     GRGLLAQARRRASQRWEFAQTALAIHLLNHENTPEMARECRVDHLQYHLRWQPDFAAT
                     VVGLSERSGLIQRSDGDLSLTDLGRERAQTALVYS"
BASE COUNT          164 a          487 c          429 g          237 t
ORIGIN      
        1 ctgcgcgtgt tggcccacga gggcctcgtg cgcgaagtgt cgcccggcgc gtgggcactg
       61 accgacgccg gcgtcatcag agcgcaggcg gcgagcgcgc ccgcccagga gtaagcccat
      121 gtcgtccact tacctcgaaa tccagctcat cgcggtcttt gtcggcgtgg cctgcgccct
      181 gccgggcgtc ttcctcgtcc tgcgccgcat ggccatgatg agcgacgcca tcagtcacac
      241 cgtcctgctg ggcatcgtcc tgggcttttt cgccattggc tcgctggagt cgccgctgct
      301 cgtcctggcc gcggcggcga tgggcgtgct gacggtcagc ctcgtcgaag tgctcatgcg
      361 cacccacctg gtgcgccagg acgcggctat cggtctggtc ttcccggccc tgttcagcat
      421 cgccgtcatc ctcatctccc gcttcgcccg cggcgtccac ctcgacgtcg acgccgtcct
      481 gctgggcgaa ctggccttcg cgccgctcga ccggatgaac ctgttcggcc tcgacctgcc
      541 gcgcagcctg gtcgtcatgg gcgtgatcat gctggccaac gcggcgctca tcgccgtgtt
      601 ctataaggaa ttgaagctga ccaccttcga cgccgggctg gcggcggcgc tgggcttctc
      661 gccggcgctg gcccactatg ggctgatgtt catggtctca gtgacggccg tgggcgcgtt
      721 cgatgccgtc ggctccatcc tcgtcgtcgc cctgatgatc gccccgccgg ccgcggccta
      781 cctgctgacc gagcgtctca gccgcatgct gctctacagc gccgccctgg ccacggccgg
      841 ggccatcagc ggcttctggt tggccgtggc cctcgacgcc aacgtcgccg gagccatcgc
      901 gacgacgctg ggggcgctct tcctgggcgt cttcctgctg gcccccggcc gcggtctgct
      961 ggcccaggcc cgccgccgcg ccagccagcg ctgggagttt gcccagacgg ccctggccat
     1021 ccacctgctg aaccacgaga acacgccgga gatggcccgc gagtgccgcg tcgatcactt
     1081 gcagtatcac ctgcgctggc agccggactt cgccgccacc gtcgtcggcc tgtctgagcg
     1141 ctctggcctc atccagcgca gcgacggcga cctgagcctg accgacctcg gccgcgagcg
     1201 ggcgcagacg gcgctggtct atagctaggg ccaccgctct gccaagcgtg gcgcgattct
     1261 ctgaatcgcg ggcctacaag agggcgattc ggagaaccgc gccacagggt gcaacgg
//
LOCUS       DAOSMQ010000659         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_102495_length_1317_cov_4.406498, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000659 DAOSMQ010000000
VERSION     DAOSMQ010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102495_length_1317_cov_4.406498"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PK829_15175"
     CDS             <1..511
                     /locus_tag="PK829_15175"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOU42609.1"
                     /translation="PDSEAYSEARRLGQLLAEAGLTVATGGYGGTMAAVSQGAAGAGG
                     HVIGVTSSHIERYRPTPPNEWVAEEIRFDSQRDRLLHLVMNNDAMIALPGGIGTLSEV
                     ALAWSLLQTGELTARPLVLLGPVWRETIRAFTQAETIRPRDLDLLYLATSSETAVAYV
                     RQSLATRGA"
     gene            529..1053
                     /locus_tag="PK829_15180"
                     /pseudo
     CDS             529..1053
                     /locus_tag="PK829_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
     gene            complement(1108..1317)
                     /locus_tag="PK829_15185"
                     /pseudo
     CDS             complement(1108..1317)
                     /locus_tag="PK829_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018808457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
BASE COUNT          169 a          427 c          497 g          224 t
ORIGIN      
        1 cccggatagc gaggcctatt ccgaggcgcg gcggctgggg cagttgttgg ccgaggctgg
       61 gctgaccgtg gccaccggtg gctatggtgg cacgatggcc gccgtcagcc agggtgcggc
      121 cggggccggc ggccatgtca tcggcgtgac ctccagccat atcgagcgct accggccgac
      181 gccgcccaat gagtgggtgg ccgaagagat tcgtttcgac tcccagcgcg accggctgct
      241 acatctggtt atgaacaatg acgccatgat cgctctgccg gggggcatcg gcacgctgtc
      301 ggaagtggcg ttggcctgga gccttttgca gacgggcgaa ctgaccgccc ggccactggt
      361 gctgctcggt ccggtctggc gcgagacgat ccgcgctttc acccaggctg agaccatccg
      421 gccgcgcgac ctcgacctgc tctacctggc cacgtcgtcg gagacggccg tggcttatgt
      481 caggcaaagc ctggccacga ggggggcgtg agccgagaag ctgtggccat gggggaggag
      541 cggtcactct ttctgaagtt aggtggctcg ctgctgaccg acaagacggc taccgaggcc
      601 ctgcgcggcg cggaactggc gcgcctggcc ggggagatcg ccgcggcgcg ggcggcacgg
      661 ctgggcttgc ggctggtgtt gggccacggc agcggctcct ttgggcacgt cgccgccgcg
      721 cggcacggca cgcgggcggg cgtggccggc gcgtccgcgt ggctgggctt cgccgcggtc
      781 agcgacgccg ccgcgcggct gaaccggcac gtcgtcgccg cgctgctggc cgccggcgtg
      841 ccggccatcg gcctgccacc ttccgcttca gctgtcgtca acgacggcca tatccgcgga
      901 ttggcgaccg gcccggtcgt cgccgccctc gatgctggct tgctgcccgt cgtcttcggc
      961 gacgtagcct ttgacaccgt ccgcggcggc acgattgtct cgaccgagga agtcctgggc
     1021 tacctggctc attcattgcg gccggcgtgg ctactgctgg ctggggagac gggcggcgtg
     1081 ctggacgccg gcggccgtgt cgtgcgaacc gccgcggatg agccagaagc cggccggacc
     1141 ggcatagacg ttcagacggg tcatgccgag ggcatggtca tggtaccaga tcgtcgccgc
     1201 gggctgcgtg ttctcgtagc tgaaatacgc cgacccgggg acggtattgg tcgtgtccgc
     1261 ctggccgtag ttcgaccctt gcatggcgta gccggcgggg atgttgttgg cggccgg
//
LOCUS       DAOSMQ010000660         1304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_104077_length_1304_cov_5.834267, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000660 DAOSMQ010000000
VERSION     DAOSMQ010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104077_length_1304_cov_5.834267"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PK829_15190"
     CDS             complement(<1..1073)
                     /locus_tag="PK829_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU42610.1"
                     /translation="MNPPAPYTDLRAYLDHNWPVYLDLLHQMVLINSFTLNPRGVNRL
                     GKLTAEHFAALGFESEAIQADGFRYGSHVTLTRHGRPAADGQPAPTIGLISHLDTVFP
                     ELEEQANDFHFRIAGDRIYGPGTVDIKGGTVLIYMILDGLRHAFPDVFESTTWHILLN
                     AAEEVLAPDFGALVRERLSGARAALIFEGGDVKDNRYQFVTARKGMARFHIEVEGRGS
                     HAGAMHERGANAVVQLADVIRAAADLTDYSRGLTFNIGTVMGGTVVNRVPHQASAGVE
                     MRAYDPDVFEDGVGRLLALSDLCTVRSVDDGYPCRVTIDIEGRWQPWPDNAGTLALFE
                     VWAAAAADLGLGTVSRQSRGGLS"
BASE COUNT          235 a          372 c          463 g          234 t
ORIGIN      
        1 tcgctcaggc cgccgcgcga ttgccggctg accgtgccca ggcccagatc ggccgccgcc
       61 gccgcccaga cctcgaacag ggccagcgtg ccggcgttgt ccggccaggg ctgccagcgc
      121 ccctcgatgt cgatggtgac ccggcagggg tagccgtcat cgacgctgcg cacggtgcag
      181 aggtcgctga gggccagcaa gcggccgacg ccgtcctcaa acacatcggg atcataggcg
      241 cgcatctcta cgccggccga tgcctggtgg ggtacgcggt tgacgactgt cccgcccatc
      301 accgtgccga tgttgaaggt caggccgcgg gagtaatcgg tcagatcggc cgccgcgcgg
      361 atgacgtcgg ccagctggac gacggcattc gcgccgcgct catgcatggc cccggcgtgg
      421 ctgccacgcc cttcgacctc gatgtgaaag cgggccatgc ccttacgggc cgtgacgaac
      481 tgatagcggt tatccttgac gtcgccgccc tcgaagatca gagccgcccg cgcgccgctc
      541 agccgttcgc ggaccagcgc gccgaagtcg ggggcgagga cttcttcggc cgcgttgagc
      601 aggatgtgcc aggtagtgct ctcgaagacg tcgggaaagg cgtggcgcag tccgtcgagg
      661 atcatgtaga tgagcaccgt cccgcccttg atatcgaccg tgccggggcc gtagatgcgg
      721 tcgccggcga tgcggaaatg gaagtcgttg gcctgctcct ccagttctgg gaagacggtg
      781 tcgaggtgag agataaggcc gatggtcggg gcgggttggc cgtcggccgc cgggcgtccg
      841 tggcgagtca gcgtgacgtg ggagccgtag cggaagccgt cggcctgaat ggcctcgctc
      901 tcgaagccca gcgcggcaaa gtgctcggcc gtcagcttgc ccaggcggtt gacgccgcgc
      961 gggttgaggg tgaagctatt gatgaggacc atctggtgca gcaggtcgag atagacaggc
     1021 cagttgtggt ccagataggc gcgcagatcg gtgtaggggg cgggggggtt catgggctag
     1081 ttatagcatt gacggcgggc agcttcaagc gaaatgagcc tcgtttttgc ctggggactt
     1141 gcattttgga acagatgtgc tatactatgt tgtagatacg caacaaatgg aacgtttgcg
     1201 tagaagatgt tatgataggc cttggcggct aagagagact ctcggccgat tgcgcaaggc
     1261 acgacttaag gagaaacgca aatgtatcag aaaatcgtga ttgt
//
LOCUS       DAOSMQ010000661         1304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_104080_length_1304_cov_3.950360, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000661 DAOSMQ010000000
VERSION     DAOSMQ010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104080_length_1304_cov_3.950360"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK829_15195"
     CDS             complement(<1..344)
                     /locus_tag="PK829_15195"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOU42611.1"
                     /translation="MAWQIDKSHTHVTFVARHMMVAKVRGEFSNYEVEVAYNEDTPSL
                     SSVKAVIYTDSINTRDERRDGHLRSADFVDAANYPTMTFESKRVEQRPNGNGRITGDL
                     TIRGVAREVVLDV"
     gene            478..1128
                     /locus_tag="PK829_15200"
     CDS             478..1128
                     /locus_tag="PK829_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU42612.1"
                     /translation="MMPRIDLTTIDEGSPAERHERADAAANRALILQTAERLFAEYGV
                     ANVCMATIAEAAGVGKGTLYRRFASKGELCLALMDSQMSTFQAEALARFRQQTRAGAS
                     YLTQLGDFLEALVFFTDRHAPLLAEVQHGGLLRTETPLNLPHFWQFMTVSALLRSAAR
                     NGELAAGLDIDYIGEALLSPLQIDIFRFQRDGRGYTLERIAAGLRGLVSLLGEHPT"
BASE COUNT          205 a          412 c          423 g          264 t
ORIGIN      
        1 acgtcgagca cgacctcgcg ggccacgccg cggatggtta ggtcgccggt gatgcggcca
       61 ttgccgttgg gccgttgctc cacgcgcttg ctctcgaagg tcatcgtcgg gtagttggcc
      121 gcgtcgacga agtcggccga gcgcaggtgg ccgtcgcgcc gctcgtcgcg cgtgttgatg
      181 ctgtcggtgt agataaccgc cttgaccgac gaaagggagg gtgtgtcctc gttataggcc
      241 acttcaacct cgtagttgct gaattcaccg cgtactttgg cgaccatcat gtggcgcgcc
      301 acaaaggtaa catgggtgtg ggatttgtct atttgccaag ccattgtcgt gctctcctct
      361 ttgtttgtgt tttgtatgtg gacttcagtc cgtttatctg ctatgatatt aacggactag
      421 agtccacttg tcaagtgcaa atcaacgtat ttcgcgtata atctaggtta gaaaaagatg
      481 atgccacgaa ttgacctgac gaccatcgac gaaggcagcc cggccgaacg ccacgagcgg
      541 gccgacgccg ctgccaaccg ggcgctgatc ctgcagacgg ccgagcgcct ctttgccgag
      601 tacggcgtcg ccaacgtctg catggctaca atcgccgagg ccgccggggt gggcaagggc
      661 acgctctacc gccgctttgc cagcaagggc gagttgtgtc tggcgctcat ggacagccag
      721 atgagcacct tccaggccga ggcgttggcg cgcttccggc agcagacgcg cgctggcgcg
      781 tcgtatctca cccagttggg cgacttcctg gaggcgctgg tgttctttac ggacaggcac
      841 gcacccctgc tggccgaggt gcagcacggc ggcctgctgc gcacggaaac gccgctgaat
      901 ctgccgcact tctggcagtt catgaccgtc agcgcgctgc tgcggtcggc ggcgcgcaac
      961 ggcgagctgg ccgccggcct ggacatcgac tacatcggtg aggctctgct gtcgccgctg
     1021 caaatcgaca tcttccgctt ccagcgcgat ggccgcggct acacgctgga acgcatcgcc
     1081 gctggcctgc gcgggctggt gagcctgctg ggcgagcatc cgacctagtt tatggacaat
     1141 ctccatcgtg cttctacggg aaggccccgc cttcctatgc tgatcctgct tgcggctttg
     1201 ctattggccg ccgtcctggc ggcgcgggcc tcagcggcgg atagcgcgtc gcccgccgcg
     1261 gccggggccg ccagcgcgcc cgccgcggcc ggggccgcca gcgc
//
LOCUS       DAOSMQ010000662         1299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_104669_length_1299_cov_4.418006, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000662 DAOSMQ010000000
VERSION     DAOSMQ010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104669_length_1299_cov_4.418006"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PK829_15205"
     CDS             complement(<1..310)
                     /locus_tag="PK829_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42613.1"
                     /translation="MNRSRSVAAAVLVAAAWLVAGLALVRLLFEWRFPAWLWLGRAGP
                     AALCGAAGAAGGLLLARRFGRAFAWASAPMLLNLLWLLDPAVDLPRGRFLFAAGWWLA
                     A"
     gene            644..>1299
                     /locus_tag="PK829_15210"
     CDS             644..>1299
                     /locus_tag="PK829_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOU42614.1"
                     /translation="MQPNKSDHFHIDTLAVHAGVAPDPTTGAVMTPIYQTSTYAQTGI
                     NEHKGYDYARTDNPTRSALQAALAALEGGRYGLAFSSGMAAIDTLARLVAPGERVICG
                     NDLYGGTFRLFDKVLAATGVRFTFVDTADPAAVAAELRRAPARLVWLESPTNPTLRLA
                     DIATISAAAHAAGAQVAVDNTFASPVLQRPLALGADFAVHSTTKYIGGHSDVVGGALL
                     T"
BASE COUNT          242 a          508 c          396 g          153 t
ORIGIN      
        1 cggcggccag ccaccagccg gcggcgaaga gaaagcgccc gcggggcaag tccacagccg
       61 ggtcgagcag ccagaggagg ttcagcagca tcggcgcgga cgcccacgcg aacgctcgcc
      121 cgaaccggcg cgccagcagt agccccccgg ccgccccggc cgcgccacag agggccgccg
      181 gcccagcccg gcccaaccag agccacgccg ggaagcgcca ctcgaacagc agccgcacca
      241 gcgccagccc ggccacgagc caggccgcgg cgacgagcac ggccgcggca acactacggc
      301 tccggttcaa gcgtatactc cgccacctgg cggccctcat tgcagagtgc ggcccgccag
      361 accccgccga ccaggagcgc caggatgagc gggcactgtt gcctgctaaa acgtttcatc
      421 gtgactccat aacagggctt tgccaaagtc aaccccaggc gcaccgacct gacaggtgga
      481 cggccgcgaa tgatactgcc accacagaac gaccggccgt ccacctgtca ggtcaatggt
      541 cgccagacga ctttggcaaa gcccctggac tccataatgg gccgatgccc tctgtggtta
      601 tgatacaata caatccgccg accaccatac aggagaggca gccgtgcagc caaacaaatc
      661 ggatcacttc cacatcgaca cgctggccgt ccacgccggc gtcgcgcccg acccgaccac
      721 cggggcggtg atgaccccca tctaccagac ctccacctat gcccagacgg gcatcaacga
      781 gcacaagggc tatgactacg cccgcaccga caacccgacg cgctcggcgc tgcaagcggc
      841 gctggcggcg ctggagggcg ggcgctacgg cctggctttc tcgtcgggca tggcggccat
      901 cgacacactg gcgcgccttg tcgccccggg cgagcgcgtc atctgtggca acgacctcta
      961 cggcggcacg ttccgcctgt tcgacaaggt gctggccgcc accggcgtgc gcttcacctt
     1021 cgtggacacg gccgacccgg ccgccgtcgc cgccgaactg agacgcgcgc cggcgcgcct
     1081 ggtctggctg gaatcgccca ccaacccgac gctgcgcctg gccgacatcg ccaccatcag
     1141 cgccgccgcc catgccgccg gggcacaggt ggccgtggac aacaccttcg cctcgcccgt
     1201 gctgcaacgg ccgctggccc tgggagccga cttcgccgtc cacagcacca ccaagtacat
     1261 cggcggccac tccgacgtcg tcggcggcgc actgctcac
//
LOCUS       DAOSMQ010000663         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_105395_length_1293_cov_4.616317, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000663 DAOSMQ010000000
VERSION     DAOSMQ010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105395_length_1293_cov_4.616317"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PK829_15215"
     CDS             <1..988
                     /locus_tag="PK829_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOU42615.1"
                     /translation="GRVVERLGPGVPVGIHTHDDAGCGVANTLAAVRAGAVQVQGTVN
                     GYGERVANANLCTIIPDLQLKMGYDCVPAERLTDLTDLSRYVAELANLTHDDHLPFVG
                     ASAFAHKGGIHVAAMLKNPGSYQHIAPEQVGNRQRSVVSELSGRGNLVDKIRQFNLNP
                     ESLDLPAVLEQIKKLEAQGFTFEGAEASVELMLRRTHPAYVPPFEMTDYWVMVQRRRG
                     RGSIVEATVKVRVGPRIMHTVAEGNGPVNALDAALREALSGVYPQISGVRLEDYKVRI
                     LDGENNTAATTRVLIETRQGTRRWSTVGASPNIIDASWRALADSMEYALVTG"
     gene            1002..>1293
                     /locus_tag="PK829_15220"
     CDS             1002..>1293
                     /locus_tag="PK829_15220"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOU42616.1"
                     /translation="MLHSPLPPSAQRVADAAAALGLTIDIVTFAQSTRTAEAAAEAVG
                     CEVGQIVKSLVFTVGGEPVMALVSGKNQLDTRKLAGLFGVGRKQVERADADTV"
BASE COUNT          224 a          463 c          424 g          182 t
ORIGIN      
        1 cggccgcgtc gtcgagcggc tggggccggg cgtgcccgtg ggcatccaca cccacgacga
       61 tgccggctgc ggtgtggcca acaccctggc cgccgtccgt gccggggccg tccaggtgca
      121 gggcacggtc aacggctatg gcgagcgcgt cgccaacgcc aatctgtgca ccatcatccc
      181 tgacctgcaa ctcaaaatgg gctatgactg cgtgccggcc gagcgcctga cagacctgac
      241 cgacctgtcg cgctacgtgg ccgaactggc caacctgacc cacgacgacc atttgccgtt
      301 cgtcggcgcc agcgccttcg cccacaaggg tggcatccac gtcgccgcca tgctcaaaaa
      361 ccccggcagc taccagcaca tcgcgccgga gcaggttggc aaccgccagc gcagcgtcgt
      421 gtcggagctg tccggccgcg gcaatctggt ggacaagatc aggcagttca acctcaaccc
      481 ggagagcctc gatctgccgg ctgtgctgga acagattaag aagctggagg cccagggctt
      541 caccttcgag ggagcggagg catcggtcga gctgatgctg cggcgcacgc acccggccta
      601 cgtgccgccg ttcgagatga ccgactactg ggtcatggtg cagcggcggc gcgggcgcgg
      661 ttccatcgtc gaggcgacgg tcaaggtgcg cgtcgggccg cgcatcatgc acaccgtggc
      721 cgagggcaat ggcccggtca acgccctcga cgcggccctg cgcgaggcgc ttagcggcgt
      781 ctacccgcaa atctccggcg tgcgcctgga ggactacaaa gtgcgcatcc tcgacggtga
      841 gaacaacacc gccgccacca cgcgcgtgct catcgaaacg cgccagggga cgcgccgctg
      901 gagcaccgtc ggagccagcc ccaacatcat cgacgccagc tggcgggcgc tggccgatag
      961 catggagtac gccctggtaa cgggctgacg gagcgccaat aatgctacat tcacccctcc
     1021 ccccttccgc ccagcgtgtg gccgacgccg ccgcggcgct gggcctgacc atcgacatcg
     1081 tcaccttcgc ccaatcgacg cgcacggccg aagccgccgc cgaggccgtc ggctgcgagg
     1141 tcgggcagat cgtcaagagc ctggtcttca ccgtcggcgg cgagccggtc atggccctcg
     1201 tgtcgggcaa gaatcaactc gacacgcgca agctggccgg cctgttcggc gtcggccgca
     1261 agcaggtcga gcgcgccgac gccgacaccg ttc
//
LOCUS       DAOSMQ010000664         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_105404_length_1293_cov_3.624394, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000664 DAOSMQ010000000
VERSION     DAOSMQ010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105404_length_1293_cov_3.624394"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PK829_15225"
     CDS             <1..999
                     /locus_tag="PK829_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42617.1"
                     /translation="LDRQGVAIAAATTLYRLGATDEGERGLLQAMRLVSSDAHNSHAR
                     YPELAWIDLRVGLGYFQLSQKLLVALVELIADESYPGRVDAEFAHLGAPPMRERARLT
                     LRSLLTGHQPGAIVALERIQEPAFRPGFVRYLEGQAATPAGRAVLAALPEAERARLRE
                     MLLHRLNAHEDNNAILVVGLLGGVEAREKQEEIVGRQHGEFDAARAAVALQYIHDDDA
                     FQLVLETIRAGRGQYDRDQTISLLAHLPLTRERLGALLLSLPDIPDNLTRVLLLDWIG
                     QRYATTAEAQALYATIKDASINKAVWNDAKGAYDFIDGQVPDEAALRTLLEMLSGF"
     gene            1156..>1293
                     /locus_tag="PK829_15230"
     CDS             1156..>1293
                     /locus_tag="PK829_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOU42618.1"
                     /translation="MDQPSLDELNLLHASVCQALGDPKRLLILYALAERPRHVSALAA
                     DL"
BASE COUNT          223 a          429 c          426 g          215 t
ORIGIN      
        1 ctggataggc agggcgtcgc catcgccgcg gcgacgacac tgtatcgtct gggcgcgacg
       61 gacgaagggg agcgcgggct gttgcaggcc atgcgcctgg tgagcagcga cgcccacaac
      121 agccacgccc gctaccctga actggcctgg attgacctgc gcgtcgggct gggctatttc
      181 caattgagcc agaagctgct ggtggctctg gtggagctga tcgccgacga gtcctacccc
      241 ggccgcgtcg atgccgagtt tgcccacctg ggcgcgccgc cgatgcgtga gcgggcgcgg
      301 ctcacgcttc ggtcactgtt aacaggccat cagccgggcg cgatcgtggc cctggagcgc
      361 atccaggagc ctgcctttcg ccccggtttc gtgcgctatc tagagggaca ggcggccacg
      421 ccggccgggc gggccgtgct ggcggcgctg cccgaggcgg agcgggcgcg gctgcgagag
      481 atgttgcttc accggctgaa cgcccacgag gacaacaacg ccatcctggt ggtgggcttg
      541 ttgggcggcg tggaggcgcg tgagaagcag gaagagatcg tgggccggca gcatggcgaa
      601 ttcgacgcgg cccgcgccgc cgtcgccctg caatacatcc acgacgacga tgccttccag
      661 ctggtcttag agactatccg cgccggccgc ggccagtatg accgcgacca gaccatcagc
      721 ttgctggctc acctgccgct gacacgggag cgcctcggcg cgttgctgct gagcttgccc
      781 gacatccccg acaacttgac gcgcgtcctg ctgttggact ggatcggcca gcgctacgcc
      841 acgaccgccg aagctcaagc tctttatgcc acgatcaagg atgcgtcgat caataaggcg
      901 gtgtggaacg acgccaaggg cgcctatgac ttcatcgacg gccaggtgcc ggacgaggcc
      961 gcgctgcgga cactactcga gatgctgtcg ggcttctgag acgacgccgc tccacggggg
     1021 aaggcgacca tctgacgcgc cgcgtcaccc atgacattcg tcacctcttc caggtgacaa
     1081 acgtcactac tgccggcgag agagcgagcg tacactgacc atgtattcgc ctatccgaat
     1141 acatggggaa agccaatgga tcagccctcg ctcgatgagc taaacctatt gcacgccagc
     1201 gtctgtcagg ctctgggcga ccccaaacgg ttgctcatcc tctacgccct ggccgagcgg
     1261 ccgcgccacg tctcggcgtt ggccgccgac ctc
//
LOCUS       DAOSMQ010000665         1291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_105644_length_1291_cov_3.746764, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000665 DAOSMQ010000000
VERSION     DAOSMQ010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105644_length_1291_cov_3.746764"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..>1291)
                     /locus_tag="PK829_15235"
     CDS             complement(291..>1291)
                     /locus_tag="PK829_15235"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOU42619.1"
                     /translation="AAIQADCNTVAGTYTTAEDGTLAITLGPSTLMACPEDSQAQAFQ
                     TGLAAAASYLFDGSDLLIDLAGDVGTLHFRPAAGAAGEGTTETEPMSLSGTVWQWVET
                     ATPQETVAAADPTRYTITFNEDGTAAIKADCNQVMATYTADGGTLSIIPGASTMALCP
                     EDSQGTAFVTALSSAATYFFQDGFLYIDQFASAGTMKFAAPEAAAGAEPGKGETVTGG
                     EGLVGPLWQLTLITKTDGNITINDPTRFTISFNADGTANLQADCNVGGANYTTGDGNT
                     LSITPTVSTMAFCGVGSFDQIFLGGLTNAMGYRLEDGNLLIDMLYESGSMVFMPAP"
BASE COUNT          205 a          425 c          431 g          230 t
ORIGIN      
        1 ggtcaaggat gtggacaaac tgctgctcga ccagctcggc cagcgccggc accacgtcgc
       61 cgttaaagcg gctgcccgga aagctgatca ctatgtattc caccggaccc aggttcatcg
      121 ttagactcct ttgctcaagg gtattaggcc ccacaccagg cgggcccgac atcattctag
      181 ccccaaacca gggcagagaa aacccccgtg tcaaaattgg ccgaccggcc ctggtggctt
      241 cctccaaccg gggccgatcg gcgctcatgc tgttgccgtc tccctagcgc ctatggcgcg
      301 ggcatgaaga ccatcgagcc gctctcatag agcatgtcga tgagcaggtt gccgtcctcc
      361 agacgatagc ccatcgcgtt ggtcaggccg cccaggaaga tctggtcgaa cgaaccgacg
      421 ccacagaagg ccatcgtcga gacggtcggc gtgatgctca gcgtgttgcc gtcgccggtg
      481 gtgtagttcg ccccgccgac gttgcagtcg gcctgcaagt tggccgtgcc gtcggcgttg
      541 aagctgatgg tgaagcgcgt cgggtcgttg atggtgatgt taccgtcggt cttggtgatc
      601 agcgttagct gccacagcgg gccgaccagc ccctcgccac cagtcaccgt ctcgcccttg
      661 cccggctccg cgccggcagc cgcctccggc gcggcgaact tcatcgtgcc cgcgctggcg
      721 aactggtcga tgtagaggaa gccgtcctgg aagaagtagg tcgccgcgct ggaaagggca
      781 gtcacgaagg ctgtcccctg cgaatcctcc gggcacaacg ccatcgtgct ggccccggga
      841 ataatgctga gcgtcccacc gtcggccgta tacgtcgcca tcacctggtt gcagtcggcc
      901 ttgatcgccg ccgtgccgtc ctcgttaaag gtgatggtgt agcgcgtcgg gtcggccgcg
      961 gcgacggtct cctgcggcgt ggccgtctcg acccactgcc agaccgtgcc ggacagggac
     1021 atcggctcgg tttcggtcgt gccctcgccg gcggcgccgg ccgcggggcg gaagtggagc
     1081 gtgcccacgt cgccggccag atcgatgagc aggtcgctgc cgtcgaacag atagctggcg
     1141 gccgcggcca ggcccgtctg gaaggcttgc gcctgcgagt cctccgggca ggccatcagc
     1201 gtcgaggggc cgagggtgat cgccagcgtg ccgtcctcgg ctgtggtata ggtgccggcg
     1261 accgtgttgc agtcggcctg gatggccgcc g
//
LOCUS       DAOSMQ010000666         1283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_106591_length_1283_cov_4.504886, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000666 DAOSMQ010000000
VERSION     DAOSMQ010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106591_length_1283_cov_4.504886"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /gene="modA"
                     /locus_tag="PK829_15240"
     CDS             <1..550
                     /gene="modA"
                     /locus_tag="PK829_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU42620.1"
                     /translation="AQMDVAVETGRIDAGAPQVFVTNRLVVVVPADNPASITDLTDLA
                     TPGVVLILAAEEVPAGRYALEILDRASDAVGVGSTFRDDVLANVVSYEENVRSVLNKV
                     VLGEADAGIVYTSDAISEPGVTAIEIPDELNVLAEYPIAVLNDSAYAEDAAAFVAYVL
                     GPEGQATLANYGFGVSSASSAQ"
     gene            547..>1283
                     /locus_tag="PK829_15245"
     CDS             547..>1283
                     /locus_tag="PK829_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU42621.1"
                     /translation="MSWLPGTGARRRPRDWPRGRRRARPHHRPETWWALALPLLLFLA
                     LPLLALLFYSPPAELRANLARPEVQQAISLSLRTSTLATLAAVLLGLPVAYLLARREF
                     RGRMLLDNLIDLPIVLPPAVAGLALLLVFGRRGWLGAPLDDLGLRVTFTQTAVVIAQT
                     FIAVSLFVRAATIGFAAVEPELEDAAAIDGAGPGQVFWRISLPLARRGVLSGLALAWA
                     RALGEFGATIIFAGNFPGRTQTMPLAI"
BASE COUNT          167 a          442 c          450 g          224 t
ORIGIN      
        1 ggcgcagatg gacgtcgccg tggagacggg ccgcatcgac gccggcgcgc cacaggtctt
       61 cgtcactaac cggctggtcg ttgtcgtccc ggccgacaac ccggccagca tcaccgattt
      121 gaccgacctg gccactcccg gcgtcgtgct catcttggcg gcggaggagg tgccggccgg
      181 acggtacgcg ctggaaattc tggacagggc cagcgatgct gtcggcgttg gctcaacctt
      241 ccgcgacgac gtgttggcca acgtcgtttc ctacgaggag aacgtgcgct cggtactcaa
      301 caaggtcgtt ctgggcgagg ccgatgcggg catcgtctac accagcgatg ccatttccga
      361 gccgggcgtg acagccatcg aaatccccga cgagttgaac gtgctggccg agtatcccat
      421 cgccgtgctc aatgacagcg cctatgccga agacgcggcg gcgttcgtcg cctacgtgct
      481 tgggccagag ggccaggcca cgctggcaaa ctacggcttt ggcgtgtcgt cggcgtcgtc
      541 ggctcaatga gttggctgcc gggcacaggg gcgcgccgtc gcccgcgcga ctggccgcgc
      601 gggcgacggc gcgcccgtcc acaccaccgg ccggagacgt ggtgggcgct ggcgttgccg
      661 ctgctgctct ttctggcgct gcccctgctg gcgctgctgt tctatagccc gccggccgaa
      721 ctgcgcgcca atctggcccg gccggaagtg cagcaggcca tcagtctgag cctgcgcacc
      781 agcacgttgg ccacgctggc cgccgtcctg ctgggcctgc ccgtggccta cctgctggcc
      841 cggcgcgagt tccgcggccg gatgctgctg gacaacctca tcgacctgcc tatcgtccta
      901 ccgccggcgg tggccgggct ggcactgctg ctggtgtttg gccggcgcgg ctggctgggc
      961 gcgccgctcg acgacctggg gctacgtgtc accttcacgc agacggccgt ggtcatcgct
     1021 cagacgttca tcgccgtctc cctgttcgtt cgcgcagcaa cgatcggctt cgcggccgtc
     1081 gagccggaac tggaagacgc cgcggccatc gacggggccg ggccgggaca ggtcttctgg
     1141 cgcatttcgc tgccgctggc gcggcggggg gtgctgtcgg gtctggccct ggcctgggcg
     1201 cgggcgctgg gcgagttcgg ggcgacgatc atcttcgccg gcaacttccc cggccgcacg
     1261 cagaccatgc cgctggcgat cta
//
LOCUS       DAOSMQ010000667         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_107110_length_1279_cov_3.508987, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000667 DAOSMQ010000000
VERSION     DAOSMQ010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107110_length_1279_cov_3.508987"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK829_15250"
     CDS             complement(<1..516)
                     /locus_tag="PK829_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42622.1"
                     /translation="MDQLGPVAVALVALWLYRGALGLAYFNDDPTGHFAWMEGRSAVD
                     FFTGSAAYGYYRPVVFTALRLFERLFGSAVWPHNPFADHALLLLLHAANTALVWSLAR
                     ALARPSGRGGAAFAWLAALLFAALPFSYEAVAYVASLTHPLVVFWVLVTLHLYLRRLT
                     ADRRRPTADRRR"
     gene            complement(580..>1279)
                     /locus_tag="PK829_15255"
     CDS             complement(580..>1279)
                     /locus_tag="PK829_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42623.1"
                     /translation="DESLLTHVRVKLSPDPTAPVLIFHHGFSELPYYNAWQRIFHRPV
                     SFPLTTVCIQAPFHNHWREPFREAFRSVRRVYQTFAGSLRLIELVQREFENAGAAYSV
                     LSGVSWGGVTSMLYAGTFGGVRAVAPMLSSPNLAQVLWDGGELVGMEMPISKPELEAL
                     LDFTPRCAYDPARVFPLLGETDVFFQVDKHAGHYATEPVIVPRAHIFSLYGVRPLRDH
                     IVRVMDWAAGHPLE"
BASE COUNT          258 a          420 c          439 g          162 t
ORIGIN      
        1 ccgtcggcgg tcggcggtcg gccgtcggcg gtcggccgtc agccgtcgca aatacagatg
       61 caacgtaacc agcacccaga agacaaccag cgggtgcgtc agcgaggcaa cgtaggcgac
      121 ggcttcgtag ctgaagggca gagcagcaaa gaggagggcc gccagccagg cgaaggccgc
      181 gccgccgcgc cccgacggcc gcgccagcgc ccgcgccagg ctccacacca gcgctgtgtt
      241 ggccgcatgc agtagcagga gaagggcgtg gtcggcaaaa gggttgtggg gccagacggc
      301 gctgccgaag aggcgctcga acaggcgcag ggccgtgaag acgaccggcc ggtaatagcc
      361 gtaggcggcc gagccagtga aaaagtccac ggccgaccgc ccctccatcc aggcgaagtg
      421 gccggtgggg tcatcattaa agtaggccag ccccagtgcc ccacgataga gccacaaagc
      481 gacgagcgcc accgccaccg gccccagctg atccacaagg cggtagacgc cttttcccgc
      541 gctcctccgc ccccctgctc ccccgcctcc gcccacggct tactccagcg gatgccccgc
      601 cgcccaatcc atcacgcgca cgatgtggtc ccgcagcggc cggacaccat agaggctgaa
      661 gatgtgggcg cgcggcacaa tgaccggctc cgtggcatag tggccggcgt gcttgtccac
      721 ctggaagaag acatcggtct cgcccagcag cgggaagacg cgcgcggggt cataggcgca
      781 gcgcggcgtg aagtcgagca gcgcctccag ttccggcttg ctgatgggca tctccatgcc
      841 caccagttca ccgccatccc acagcacctg ggccagattg ggcgaggaga gcatgggggc
      901 cacagcgcgg acgccgccga acgtgcccgc gtagagcata ctggtgaccc cgccccagct
      961 gacgccagaa aggacactgt aggctgcgcc ggcattctcg aactcgcgtt gcaccagttc
     1021 gataagccgc aacgacccag cgaacgtctg atagacgcga cgcacggagc gaaaagcttc
     1081 gcgaaacggc tcgcgccagt ggttgtggaa aggggcctgg atgcagaccg tcgtcagcgg
     1141 gaaggacact ggccggtgga agatgcgctg ccaggcgttg taatagggca gttcgctgaa
     1201 gccgtggtgg aagatgagca cgggcgcggt gggatcgggg ctgagcttga cgcggacatg
     1261 ggtcaacagc gactcgtcg
//
LOCUS       DAOSMQ010000668         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_107143_length_1279_cov_2.562092, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000668 DAOSMQ010000000
VERSION     DAOSMQ010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107143_length_1279_cov_2.562092"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1279)
                     /locus_tag="PK829_15260"
     CDS             complement(<1..>1279)
                     /locus_tag="PK829_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42624.1"
                     /translation="THLWEALKRLFQYGWDGLVNTFKVFQALFTGDWEELGRLLRERF
                     ENTIDAISDIMWHLWDAVGPALIDLAARIVTWWNGIDWRALARKALEELKRGLAEGGQ
                     GGGDDPLRLGGLMTGGAMSSGYEAGQRARAAFVKGWQEEGVAADEIFSPEATREQNRL
                     AVAAKKVAQEAAGADRVLGQYAQGINAITRSSDRASGNIRALDLYLAQTRDHLKLDAE
                     RSLHFAAGIDALTAAAARNKAGLDAAAQAAQSYATAFAGVQADYVTELPQADEPMVNP
                     EHDVTIRTRISGPTAEQAELAARYNDELEKLRETYAELTGGVGTFGMEQDKLDEKIGA
                     VAAEMDYYQGLLDGIPPTVNDVSAAQQGLAVNVDAVHRALYDQLVQMGAAPEVIAAYA
                     AATGIMSEEQAEAALVAARLQVEIEALATKMAEN"
BASE COUNT          183 a          491 c          376 g          229 t
ORIGIN      
        1 gttctcggcc atcttcgtcg ccagcgcctc aatttcgact tgcagccggg ccgcgacgag
       61 cgccgcctcg gcctgctcct ccgacatgat gcccgttgcc gcggcgtagg cggcaatcac
      121 ctccggcgcg gcccccatct gcaccagctg gtcatagagc gcccggtgca ccgcgtccac
      181 attgaccgcc aggccctgct gcgccgccga cacgtcattg accgtcggcg gaatcccgtc
      241 gagcagaccc tggtagtagt ccatctcggc ggcgaccgcg ccgatcttct cgtcgagctt
      301 gtcctgctcc atcccgaacg tgcccacccc gccagttagc tcggcgtagg tctcgcgcaa
      361 cttctccagt tcgtcgttgt accgcgccgc tagttctgcc tgctcggccg tcggcccgct
      421 gatgcgcgtc cggatggtca cgtcatgctc tggattgacc atcggctcat cggcctgggg
      481 cagttcggta acgtaatcgg cctgcacccc ggcaaaggcc gtcgcgtagc tctgcgccgc
      541 ctgcgcggcc gcgtccagtc cggccttgtt tcgcgccgcc gcggccgtca gcgcgtcgat
      601 gcccgcggcg aagtggagcg accgctccgc gtccagcttc aggtgatcgc gagtctgggc
      661 gagataaagg tcaagcgcgc gaatattgcc actggccctg tcgctggagc gggtaattgc
      721 gttaatgccc tgcgcgtact ggccgagcac gcggtcggcc ccggccgcct cctgagccac
      781 cttcttggcc gccaccgcca gccggttctg ttcccgcgtc gcctccggcg agaaaatctc
      841 atccgcggcg acgccctctt cctgccagcc cttcacgaag gccgcgcggg cgcgttgccc
      901 ggcttcgtag cccgacgaca tcgcgccgcc ggtcatcagc ccgcccagtc gcaacgggtc
      961 atccccgcca ccctggccgc cctccgccaa cccccgcttc agttcctcca gcgccttgcg
     1021 ggcgagcgcc cgccagtcga tgccgttcca ccacgtgaca atccgcgcgg ccaggtcaat
     1081 cagcgccggg ccaaccgcgt cccatagatg ccacatgatg tcgctgatcg cgtcgatggt
     1141 gttctcaaaa cgctcgcgca acagccggcc gagttcctcc cagtcgccgg tgaacagcgc
     1201 ctgaaagacc ttgaacgtgt tgaccaggcc atcccagccg tactggaaca ggcgcttcag
     1261 cgcctcccac aggtgggta
//
LOCUS       DAOSMQ010000669         1278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_107238_length_1278_cov_3.532298, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000669 DAOSMQ010000000
VERSION     DAOSMQ010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107238_length_1278_cov_3.532298"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..>1278
                     /locus_tag="PK829_15265"
     CDS             152..>1278
                     /locus_tag="PK829_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42625.1"
                     /translation="MYALLALLAAGSTWVLLNWLDGAGGRWAVAYVLLTAAGLYTHYF
                     FPAVVAAQGLIVMGRRRPEGRRPTTDGRPRPEGRSDTVGGRLSAVGGRLSAVVHWLLL
                     AAAAALLYLPWLPIFLRQVGGRAGQRAGLLAFLADSGRWLVLGQTIGRGEALWALVAA
                     GLLCLLGLLAARHWGTMFGLIVVVPLGLMFFVGATDPAFFKFLLVAAPFVCLLMGLAW
                     VLPRRWLWLPAVLTALVLAGSVASLRHLYADPAYARADYRGIAARIAAEGGNAGIILV
                     APNQWEVFTYYHRDGAPVYPLPTGQPDPAIVEPELARIAAAHERLYVLYWGEQQRDPA
                     GIIERWLDANTFKASEEWVGDVRLAVYAVSPLAGAAAPIAPT"
BASE COUNT          142 a          455 c          439 g          242 t
ORIGIN      
        1 cgacggccga ccgcggccag aaagggtcag tgggtcagtg ggtcagtggg tcagtgggtc
       61 ggccgtcggc ggtcgtcttg cggcggtcgt tcctgctgtt cttctcgccg ccgtcgcgcc
      121 cgtcctggtc tactacagcc aggaaacgcg catgtacgcc ctcctggccc tgctggccgc
      181 gggcagtacg tgggtcttgc tcaactggtt ggacggggcg ggcggacgct gggccgtggc
      241 ttacgtcctg ctgaccgccg ccggcctcta cacccactat ttcttccccg ccgtcgtggc
      301 cgcgcagggc ctaatcgtca tgggccgacg gcggccagag ggccgacggc cgaccaccga
      361 cggccgaccg cggccagaag gaagaagtga cacggtcggt ggtcgtctgt cagcggtcgg
      421 cggtcgtctg tcggcggtcg tccattggct cctcctcgcc gccgcggccg cgctcctcta
      481 cctgccgtgg ctgcccatct tcctgcggca ggtcggcgga cgggcggggc agcgggccgg
      541 gctgctggcc ttcctggccg atagcggccg ttggctcgtg ctgggccaga ccatcggccg
      601 gggggaggcc ttgtgggcct tggttgccgc gggtctattg tgtctgctgg ggttgctcgc
      661 cgcgcggcat tggggaacga tgtttggctt aatagtggtc gtgccactcg gtctcatgtt
      721 tttcgtcggc gcgaccgacc cggccttctt caaattcctg ctcgtcgccg cgccatttgt
      781 ctgcctgctg atgggcctgg cctgggttct gccgcggcgc tggctgtggc tgccggccgt
      841 gctgaccgcg cttgttctgg ccggcagcgt cgcctcactg cgccacctct acgccgaccc
      901 ggcctacgcc cgcgccgact accgtggcat cgccgcccgc atcgccgctg aaggcggcaa
      961 cgccggcatc atcctcgtcg cccccaatca gtgggaggtc ttcacctatt accaccgcga
     1021 cggcgcgccc gtctatccgc tgcccaccgg ccaacctgac cccgccatcg tcgagccgga
     1081 gctggcccgc atcgccgcgg cccacgagcg gctctatgtg ctctactggg gcgagcagca
     1141 gcgcgacccg gccggtatca tcgagcgctg gctagatgcc aacaccttta aggcctccga
     1201 ggagtgggtc ggcgatgtgc gcctggcggt ctacgccgtg tccccgttgg ccggcgcggc
     1261 cgcgcccatc gcgccaac
//
LOCUS       DAOSMQ010000670         1270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_108232_length_1270_cov_4.916872, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000670 DAOSMQ010000000
VERSION     DAOSMQ010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108232_length_1270_cov_4.916872"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PK829_15270"
     CDS             complement(<1..992)
                     /locus_tag="PK829_15270"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOU42626.1"
                     /translation="MRYTAWRAALPAALLIAALVTLCAASVRAQSAVEYPTGTWSAQF
                     WENTELSGPPASTSTTNSLHFRWGLGRPLEGQDTFSVRWTRRSWFPGAVHRFVAAMDD
                     GMRVWIDNQLIIDAWGMSAEHVVSTDVPLSEGWHNVRVDYHDLGGTATATLDWSPIAG
                     GNTYPNWKGEYFANPSLAGAPVVVRDDAFLDFSWGFGSAALNLPADNFSARWTRTFNG
                     VPGQYRLSLTSDDGARLYVNGQLVIDNWGVQAARTKSVDYWFGGATPLRVEYFEAAGT
                     ASLRLDIIPVPGGEGLLPPTPTPTPVPPMGDTVTCSVLPTASQGVVISARPLNVR"
BASE COUNT          225 a          380 c          447 g          218 t
ORIGIN      
        1 cggacgttga gcggccgggc gctgatgacg acgccctggc tggccgtggg caagacggag
       61 caagtgacgg tgtcgcccat aggcggcacg ggcgtgggcg tgggcgtcgg cggcagtagc
      121 ccttcgccgc cgggcacggg gatgatatcc aggcgcagcg aggccgtgcc cgcggcctcg
      181 aagtactcca cgcgcagcgg ggtcgcgccg ccgaaccagt agtcaaccga ctttgtccgc
      241 gccgcctgca cgccccagtt gtcgatgacc agttggccat tgacgtatag gcgcgcgccg
      301 tcgtcgctgg tcaggctgag gcgatattgg ccggggacgc cgttgaatgt gcgcgtccaa
      361 cgggcggaga agttgtcggc cggcaggttc agcgccgccg agccgaagcc ccagctgaag
      421 tcgaggaagg cgtcatcgcg gacgacaacc ggcgcgccgg ccagcgacgg gttggcgaaa
      481 tactcgccct tccagttggg ataggtgttg ccaccggcga tgggcgacca gtcgagggtg
      541 gccgtggctg tgccgcccag gtcgtggtaa tccacgcgga cgttatgcca gccctcgctg
      601 agggggacgt ctgtggagac aacatgctcc gcgctcatgc cccaggcgtc gatgatgagc
      661 tggttgtcga tccagacgcg catgccgtcg tccatagcgg ccacaaagcg gtgcaccgcg
      721 ccggggaacc aggagcgccg cgtccagcgc acggagaagg tatcctgacc ctccagcggc
      781 cggcccaggc cccaacggaa atggaggcta ttggtggtgc tggtcgatgc gggtgggccg
      841 ctcagttcgg tgttctccca gaactgggcg ctccaggtgc cggtgggata ctcgaccgct
      901 gactgggcgc ggacggaagc ggcgcacaaa gtgaccagag ccgcgatgag tagtgcggcc
      961 ggcagggcgg cgcgccaggc agtatatcgc ataggtcttc tccttatggc gattcgcggt
     1021 cgcagtcggc agcatcggcc gggcttgggc agtagtagac ttgctcactt ctatcttaat
     1081 ggcaaagggc aaccggtgtc aacagcggtc gcgcctatgg ggtgcctctg gcttttcagt
     1141 tgtcaccaat taacttgaag cctgaccggc tgtatgctgc agtagcacag cctgtcaggc
     1201 tgtcgtcgcc agccgcagcc taataggaca ttaacaaatt aacaggcatt taggttaagc
     1261 tcctcagcag
//
LOCUS       DAOSMQ010000671         1268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_108470_length_1268_cov_6.265458, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000671 DAOSMQ010000000
VERSION     DAOSMQ010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108470_length_1268_cov_6.265458"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PK829_15275"
     CDS             <1..1133
                     /locus_tag="PK829_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOU42627.1"
                     /translation="GSPVLNDQWQVVALHHAGVKKRDSAGRVLAVDGSVWQPAMGDDR
                     IAYVANEGVRVSSIMAALRAALDAGAFGGEAAALLDELFAEPPAATPAGGPARVLLTA
                     QRDLESFLAARGYDPRFLGPRVELPQLSPAQLADVAERSDSGGHVLDYVHFSLIMCRS
                     RRMAYFTAVNIDGRKTKSITRDRDVWYFDPRLPIEQQIGPDLYARNDLDQGHLVRRTD
                     PVWGRSAATANEDTFHFTNCAPQHARLNRRTWLALEDYILSNADNHDLKVTVFTGPVF
                     RADDMTYRGVYRLPAEFWKVVVMVKEDRRLSATAYLQTQKNLLEDLEFAYGPYRTYQV
                     AVSRIEAITGLDFGRLRDFDPLADTESAGPARVIGEAADVRL"
BASE COUNT          209 a          455 c          419 g          185 t
ORIGIN      
        1 ccggctcgcc ggtgctcaac gaccagtggc aggtcgtcgc cctgcaccac gccggggtca
       61 agaagcgcga ctcggccggg cgcgtgctgg ccgtcgacgg ctcggtgtgg cagccggcca
      121 tgggcgacga ccgcatcgcc tacgtggcca acgaaggcgt gcgcgtcagc agcatcatgg
      181 ccgcgctgcg tgccgcgctg gacgcgggcg cgtttggcgg cgaggcggcg gcgctgctcg
      241 atgagctgtt cgccgagccg ccggccgcca cgcccgccgg cggcccggcg cgcgtcctgc
      301 tcaccgccca gcgcgacctg gagtcctttc tggcggccag gggctatgac ccgcgcttcc
      361 tggggccgcg cgtcgaactg ccgcagctct cgccggcgca actggccgac gtggccgagc
      421 gcagcgacag cggcggccac gtgctcgact acgtccactt ctcgctgatc atgtgccgtt
      481 cgcggcgcat ggcctacttt accgccgtca acatcgacgg ccgcaaaacc aagtcgatca
      541 cccgcgaccg ggacgtgtgg tacttcgacc cgcgcctgcc catcgagcag cagatcggcc
      601 ccgacctcta cgcgcgcaac gacctcgacc agggccatct tgtccgtcgc accgaccccg
      661 tctggggccg ctcggcggcc acggccaacg aggacacgtt ccacttcacc aactgcgccc
      721 cgcaacacgc ccgtctcaac cggcgcacct ggctggcgct ggaggactac atcctcagca
      781 atgccgacaa ccacgacctg aaggtgacgg tcttcaccgg gccggtcttc cgcgccgacg
      841 acatgaccta tcgcggcgtc taccggctgc cggccgagtt ctggaaggtc gtcgtcatgg
      901 tcaaagaaga ccgccgcctg tcggccacgg cctacttgca gacacagaag aacctgctgg
      961 aagacctgga gttcgcctat ggcccctatc gcacctatca ggtggccgtc agccgcatcg
     1021 aggccattac cggactggac ttcggccgcc tgcgcgactt cgaccccctg gccgacacgg
     1081 agagcgccgg gccggcccgg gtgatcggcg aagcagcaga cgtgcggttg taggacgggt
     1141 tggcaacccg tccccagaga gccgacctga caggtgggcg gcgaacggta gcctgatagc
     1201 gacgaggctt ggggccgccc acctagtatg tccctatagg ggtagaatag gaagggaaac
     1261 aggatggg
//
LOCUS       DAOSMQ010000672         1268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_108473_length_1268_cov_4.231657, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000672 DAOSMQ010000000
VERSION     DAOSMQ010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108473_length_1268_cov_4.231657"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..>1268
                     /locus_tag="PK829_15280"
     CDS             180..>1268
                     /locus_tag="PK829_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42628.1"
                     /translation="MADESLPVTFSPEEEIVPAARADGRARARTLATGAAVFLTAVAL
                     FGLVQLATPGIVGNDGYYHIKMAYLIRQQGLTPAFEALPYTILNAGAYYDHHLLFHLL
                     LAPFAAVDPAVDGGAALTQGAKLATVLLPALGVVMAWWLLRGQNVPYAAGWALALFAV
                     SEAFLFRMSMARAQSGAVVLLLLALHWLLTGRLRWLAPLGFAFVWFYNAFPLLLVVAG
                     VYVVAVALLERRVAWGALLFPAAGIVLGLLLNPYFPRDLVFLVGHLLPKVSASAVPVG
                     NEWSPYETWTIVQNSGGALAAVLLGALALGWREERIDRAALVGLGLVGVFGLMFFRSR
                     RFVEYFPVFALLFLAFAAAPLLRDWRARP"
BASE COUNT          162 a          444 c          441 g          221 t
ORIGIN      
        1 cggcggcgac aacagcaaca acaacggcgg cgacaacagc aacgacaacg gcggcggcga
       61 caacagcaac gacaacggcg gcggcgacaa cggcaacgac aacggcggca acaatgaaaa
      121 cggcgatgcc atcgtcaccg actaggcagc cagtggccac agcaaggcga agatgaccaa
      181 tggctgatga atccctcccc gtcacgttct cccctgagga ggaaatcgtt ccggccgcgc
      241 gggccgacgg gcgcgcgcgg gcgcggacgc tggccaccgg cgcggccgtc ttcctgaccg
      301 ccgtggctct gtttgggctg gtgcagctgg ccacgccggg catcgtcggc aacgatggct
      361 actaccatat caagatggcc tatctcatcc gccagcaggg gctgacgccg gccttcgagg
      421 ccctgcccta caccatcctc aacgccgggg cctactacga ccaccacctg ctcttccacc
      481 tgctgctggc tcccttcgcc gcggtcgatc cggccgtgga cggtggcgcg gcgctaacac
      541 agggggccaa gctggccacg gtgctgctgc cggcgctggg cgtggtcatg gcctggtggc
      601 tgctgcgcgg gcagaacgtg ccctatgccg ccggctgggc gctggccctc ttcgccgtgt
      661 cggaggcgtt cctgttccgc atgagcatgg cccgggcgca atcgggcgcg gtggtgctgc
      721 tgctgctggc gctccactgg ctgctcaccg ggcgactgcg ctggctggcc ccgctcggct
      781 tcgccttcgt ctggttctac aacgccttcc cgctgctgct cgtcgttgcc ggggtctacg
      841 tcgtcgctgt ggccctgctg gagcggcgcg tggcctgggg ggcgttgctc ttcccggccg
      901 cgggcatcgt cctggggctg ctgctcaacc cctacttccc gcgcgacctc gttttcctcg
      961 tcggccacct gctgccgaag gtcagcgcgt cggccgtacc cgttggcaac gagtggtcgc
     1021 cctacgagac gtggaccatt gtccagaact ccggcggggc gctggcggcc gtgctgctgg
     1081 gggcgctggc gctgggctgg cgcgaggagc gcatcgaccg ggcggcgctg gtgggcctgg
     1141 ggctggtggg cgtcttcggg ctgatgttct tccgctcgcg ccgcttcgtg gaatacttcc
     1201 ccgtcttcgc cctgctcttt ctggccttcg cggccgcgcc gctgctgcgg gattggcggg
     1261 cgcggccg
//
LOCUS       DAOSMQ010000673         1262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_109246_length_1262_cov_4.510356, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000673 DAOSMQ010000000
VERSION     DAOSMQ010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109246_length_1262_cov_4.510356"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..>1262)
                     /locus_tag="PK829_15285"
     CDS             complement(156..>1262)
                     /locus_tag="PK829_15285"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOU42629.1"
                     /translation="AAQFYFRDMTVLKVMFGAIVTAMVLIFLTSGLGLLDYSLVYVNE
                     TYLWPGILGGLIMGVGFILGGFCPGTSLVSAAVGKIDGLIFVLGVLFGIFAFGETVGF
                     YEQFWHSSYFGRLTIPEWLGLPYGVVVVGIVLMALFMFWGAEHLERIFGGRDLSKEPK
                     WRLYAGGGLLGMALLVAFIGQPTTEERWARLEATEMARLANREVQIHPAELAVTMTDD
                     TLQTIILDVRSEADFNLFHLQEARHAPLAVLPTMLDELRAAPANTVVVVVGNDEVAAT
                     EAWKYLRAESVRSVYILEGGVNNWLRTFGDETFVAESAIAGAADDTLAFHFPTAIGAG
                     YAFAVPNEALAAGIEYTPRIVLEVKRGPGGGGCG"
BASE COUNT          252 a          444 c          383 g          183 t
ORIGIN      
        1 gcagccgcca gagggccgtg acgaacgtgt tcatacaaac agtatagatc gccgcggcgc
       61 ggggcacaag ccccccaatc cacgactggg cgacgcccgc gcgacgcctc cccaccgccc
      121 ggcccgcgcc ccggtcgcgc tccgtttcgc cgcgtctagc cgcagccgcc gccgcccggc
      181 ccgcgcttca cttccagcac gatccgcggc gtgtactcga tgcccgcggc cagagcctcg
      241 ttgggcacag caaacgcgta gcccgcgcca atcgccgtcg ggaagtggaa ggccagcgtg
      301 tcgtcggccg cgccggcaat ggcgctttcg gccacgaatg tctcgtcgcc aaaggtgcgg
      361 agccagttgt tgacgccgcc ctccagaatg tagacgctgc gcacgctctc ggcgcgcagg
      421 tacttccatg cctccgtggc cgccacctca tcgttgccga cgacaacgac gacagtattg
      481 gccggcgcgg cgcgcagttc gtcgagcatt gtcggcagca ccgccaatgg cgcatgtcgc
      541 gcttcctgca agtggaagag gttgaaatcc gcctccgagc gcacgtcgag gatgatcgtt
      601 tgcagcgtgt cgtcggtcat cgtcacggcc agttcggcgg ggtggatctg cacctcgcga
      661 ttggccagcc gggccatctc cgtcgcctcc aggcgcgccc aacgctcctc ggtcgtgggc
      721 tggccgatga aggccaccag cagggccatg cccagcaggc cgccgccggc gtagagccgc
      781 cactttggct ccttgctcag gtcacggccg ccgaagatgc gctccaggtg ttccgctccc
      841 cagaacatga acagagccat gagcacaatg ccgaccacaa cgacgccata gggcaacccc
      901 agccattcgg gaatggtcag ccggccgaag taggacgagt gccagaactg ctcatagaaa
      961 ccgaccgtct cgccaaaggc aaagatgcca aacagcacgc ccaggacgaa gatcaacccg
     1021 tcgattttgc ccacggcggc cgagacaagc gacgtgccgg ggcaaaagcc gcccaggatg
     1081 aagccgacgc ccatgatcag ccctccgagg atgcccggcc agaggtaggt ctcattgacg
     1141 taaaccaggc tgtaatccaa tagccccaac ccagaggtga gaaaaatcag caccatcgcc
     1201 gtgacaatgg ccccgaacat gaccttgagc acggtcatgt cgcggaagta gaactgcgcg
     1261 gc
//
LOCUS       DAOSMQ010000674         1257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_109922_length_1257_cov_2.797005, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000674 DAOSMQ010000000
VERSION     DAOSMQ010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109922_length_1257_cov_2.797005"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PK829_15290"
     CDS             complement(<1..742)
                     /locus_tag="PK829_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HOU42630.1"
                     /translation="MNPKLISTRALHLLSVPMLLALLLGGLSPVLAQRDDPENIPPPD
                     SVIIAGTLQTPLGCPGDWNTQCEDTMLAYDADGDLWLATFVLPAGEYEYKAALNGTWD
                     DNYGLGAEYYGPNIPLVVAEDGPVTFWYDHRSRWVADSVNNSLLSVFGDFQDELGCPA
                     DDTPECLRGLLLDPDGDGVYEYITAAVPPGDYSAAVAMSAGDETGALASEATVGDAVA
                     FTVGEGEAVLFSYDAAAGTLTTAAADPAA"
     gene            769..>1257
                     /locus_tag="PK829_15295"
     CDS             769..>1257
                     /locus_tag="PK829_15295"
                     /inference="COORDINATES: protein motif:HMM:NF014088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HOU42631.1"
                     /translation="MRAQSRSGGPAERAIASIMAELAADDESIVIDGDPACRGGVYVL
                     RLAVGVPLLVRFGRYAGGQPVAVPAGECLYAGSALGARGAVALAGRLLRHATRSGGRP
                     AHALREPLRARLAAAGLAASLPAGKTLRWHIDYLLDEAAVALRGVVALRTATRLEGVL
                     VER"
BASE COUNT          172 a          430 c          463 g          192 t
ORIGIN      
        1 cggcggccgg gtcggccgcg gccgtggtca gcgtgccggc ggcggcgtca tagctgaaca
       61 gcaccgcttc gccctcgccg acggtgaagg ccacggcgtc gccgaccgtc gcctcagagg
      121 ccagcgcgcc agtctcgtcg ccggcgctca tggccaccgc ggctgagtag tcgcccggcg
      181 gcacggcggc ggtgatgtac tcatagacgc catcgccgtc aggatcgagc agcaggccgc
      241 gcaggcactc cggcgtgtcg tcggccgggc agcccagctc gtcctggaag tcgccgaaga
      301 cgctcagcag gctattgttg acactgtcgg ccacccagcg cgagcggtgg tcataccaga
      361 acgtcaccgg cccgtcctcg gccaccacca ggggaatgtt ggggccgtag tactccgcgc
      421 ccaggccata gttgtcgtcc cacgtgccgt tcagggcggc cttgtactcg tactcgcccg
      481 ccggcagcac gaacgtcgcc agccacaggt cgccgtcggc atcgtaggcc agcatggtgt
      541 cctcgcactg cgtgttccag tcaccggggc agcccagcgg cgtctggagt gtgccggcaa
      601 tgatgactga atcgggcggc gggatgttct ccgggtcgtc gcgctgggcc aggacgggcg
      661 acaggccgcc aagcagcaac gccagaagca ttggcacgga cagcaggtgc aacgcgcggg
      721 tagaaattag ctttgggttc atcacgactt ccttggataa tcggccccat gagggcacag
      781 tcgagatcgg gcggcccggc cgagcgggcg atagcctcca ttatggcgga gttggccgcg
      841 gatgacgaat cgattgtcat cgacggcgac ccggcgtgtc gcggtggggt ctacgtcttg
      901 cggttggccg tgggcgtgcc gctgctggtg cgctttggcc gctacgcggg cggccagcct
      961 gttgccgtcc ccgccggcga gtgcctctac gccggctcgg cgttgggggc gcgcggcgcg
     1021 gtggcgctgg ccgggcggct gttgcgccac gccacgcgca gcggcggacg ccccgcccac
     1081 gccctccgcg agccgctacg ggcgcgattg gccgccgctg gcctcgccgc ctccctgccg
     1141 gccggcaaga cgctgcgctg gcacatcgac tacctgctcg acgaggcggc cgtggcgctc
     1201 agaggggtgg tggccctgcg cacggcgacg cggctggaag gggtgctggt cgagcgg
//
LOCUS       DAOSMQ010000675         1251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_110731_length_1251_cov_4.834448, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000675 DAOSMQ010000000
VERSION     DAOSMQ010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110731_length_1251_cov_4.834448"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PK829_15300"
     CDS             <1..750
                     /locus_tag="PK829_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42632.1"
                     /translation="AGFANAPHLAVSLLANGSVPGVTYRDEDVLRYTGGWSMLFDGSA
                     HALPGDVDALAILSDGSLLLSPDAPVKNLPGIGSAAVDDSDIVRYVPATGQYSWYFDG
                     SDVGLTTTAEDIDALAVLPDGDLLISTLGAAAVPGVSAGDEDVLRFHGTTGSKVTSGT
                     WSLYIDGSDLSAALGDIDAIAISGSDVYLSAEAAVTLGGQSMAPGDVFLCAGLTPGSA
                     TSCASISRVWQGTAAGLAAGANVDALELLEP"
     gene            833..>1251
                     /locus_tag="PK829_15305"
     CDS             833..>1251
                     /locus_tag="PK829_15305"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HOU42633.1"
                     /translation="MTTNNQVNTAPRASATGQPWWLLAGDWLVLLLFVFIGQRDHGIA
                     GQGALASLLSTTISLVVAWTAVALVLGGYRLRPDPGWRVWLGRVANIWLVAAPLGLVL
                     RALLRGQDAIPVIFILVALSIGGLMMVGWRAAAYWWLR"
BASE COUNT          167 a          413 c          459 g          212 t
ORIGIN      
        1 gccggttttg ccaacgcgcc ccatttggcc gtcagcctgc tggccaacgg cagcgtgccc
       61 ggcgtgacct accgggatga ggacgtgctg cgctacaccg gcggctggtc gatgctattt
      121 gacggctcgg cccacgcgct gcccggcgac gtggacgcgc tggcgattct cagcgacggc
      181 tcgctgttgc tcagccccga cgcgccggtg aagaacctgc ccggcatcgg cagcgccgcg
      241 gtcgacgaca gcgacattgt ccgctatgtg ccggcgacgg ggcagtatag ctggtacttc
      301 gacggctccg acgtgggcct gacgacgacg gcggaggaca ttgacgccct ggcggtgctg
      361 cccgacggcg acctgctgat cagcacgctg ggggcggccg ccgtgccggg cgtctcggcc
      421 ggcgatgagg acgtactgcg cttccacggc acgaccggct cgaaggttac cagcggcaca
      481 tggtcgctct acatcgacgg cagcgacctg agcgcggcgc tgggcgacat cgacgccatc
      541 gccatcagcg gcagcgacgt ctacctgtcg gccgaggcag cggtgacgct gggcggccag
      601 agtatggccc ccggcgacgt cttcctctgc gccgggctga cgccgggcag cgccacgagt
      661 tgcgcgtcca tcagccgcgt ctggcagggg acggccgcgg ggctggccgc cggggccaac
      721 gttgatgcgc tggagttgct agagccataa taggcgcgga tgacctgaca ggtctgctga
      781 gacctgtcag gtctctctgg cgcgtccgcg caaatcgggc tacaactcgc ccatgaccac
      841 caacaatcaa gtcaacacgg ctccgcgagc gagcgcgacg gggcagccct ggtggctgct
      901 ggccggcgac tggctcgtgt tgctcctctt tgtcttcatc ggccagcgcg accacggcat
      961 cgccgggcag ggggcgttgg ccagcctgct ttcgacgacg atcagtcttg tggtggcctg
     1021 gacggcggtt gccctcgtgc tgggcggcta ccggctgcgg cccgatccag gctggcgggt
     1081 gtggctcggc cgggtggcca acatctggct ggtggccgcg ccgctggggc tggtgttgcg
     1141 cgcgctgttg cgcggccagg acgccatccc ggtgattttc atccttgtcg ccctgagcat
     1201 cggcgggctg atgatggtcg gctggcgggc ggcggcgtac tggtggctgc g
//
LOCUS       DAOSMQ010000676         1242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_111898_length_1242_cov_3.834878, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000676 DAOSMQ010000000
VERSION     DAOSMQ010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111898_length_1242_cov_3.834878"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PK829_15310"
     CDS             complement(<1..794)
                     /locus_tag="PK829_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU42634.1"
                     /translation="MMQPATIDLAAVEPVLAKYQGRGREALLPLLHEAQAIHGWLPRE
                     VLAAVGQTLHVPLSDVHGVVEFYSMFYNEPMARRVIRVCFDPACHMAGSPAVRLAIEE
                     RLGLRPGETSADGRISYECVPCLGMCEHAPNALNGDRPAGDLTPADADAFLAGTYPEP
                     EPKIYGGPLIKLARAGKIDPLSLSAFEKYGGYKGLRKAMTMTPEEVLAILKGSDVLGR
                     GGAMFPVGLKWEMTRNAPGHPAEKHVIANADESEPGTFKDRALMEQ"
     gene            complement(932..>1242)
                     /locus_tag="PK829_15315"
     CDS             complement(932..>1242)
                     /locus_tag="PK829_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42635.1"
                     /translation="GAATEFAQWQLTTQLQESPPRGAVAVPRDLYLRQALFHESHGRA
                     GPAILAYEQAIVAGAQPPAIFFALGLLYRLVNRRDDARAALTLAARHPFYRRAVALLE
                     "
BASE COUNT          197 a          386 c          442 g          217 t
ORIGIN      
        1 tcttgctcca tcagcgcccg gtctttgaac gtgcccggct cgctctcgtc ggcgttggcg
       61 atgacgtgct tctcggccgg gtggccgggc gcgttgcggg tcatttccca cttcaggccg
      121 acggggaaca tggccccgcc acgaccaagc acgtcactgc ctttgaggat ggccaggacc
      181 tcctccggcg tcatggtcat ggccttgcgc agacccttgt agccgccgta cttctcgaag
      241 gcgctcaggc tgagcgggtc aatcttgcca gcgcgggcca gcttgatgag cggcccacca
      301 tagatcttcg gctccggctc cggataggtt ccggccagga aggcgtccgc gtcggcggga
      361 gtcagatcgc cggccggccg gtcgccgttg agggcgttcg gcgcgtgttc gcacatgccc
      421 agacagggga cgcactcata gctgatgcgg ccatcggcgc tggtttcgcc cggtcgcagg
      481 ccgagacgct cctcaatggc caggcgcacg gccgggctgc cggccatgtg gcaggcgggg
      541 tcgaagcaga cgcgaatgac ccggcgcgcc attggctcgt tgtagaacat cgaatagaac
      601 tcgaccactc cgtggacgtc tgagaggggg acgtggagcg tctggccgac ggccgccagt
      661 acttcgcgcg gcagccagcc atgaatggcc tgggcctcgt gtagaagagg cagcaaggct
      721 tcccgaccgc ggccctggta tttggccaat accggttcca cagcggccag gtcgattgtt
      781 gccggttgca tcatccttgc gcctcctctt taaggaacaa gcgaatgtcc gttcgcggtt
      841 gtgggagtag gccgaagcct ctacctgccg gttgggggcc gggaggtagg gcaacattat
      901 atagcaggat cgtccgcggg cgaacggatt cttattctag caaggccact gcccggcgat
      961 agaaggggtg gcgcgcggcc agcgtcaggg ccgcgcgggc gtcgtcgcgg cgattgacca
     1021 gccggtagag cagccccagg gcgaagaaga tggccggcgg ttgcgccccg gcgacgatgg
     1081 cttgctcata ggcgagaatg gccgggccgg cgcggccgtg gctctcgtgg aagagggcct
     1141 ggcgcaggta gagatcgcgg ggcaccgcca cggccccacg cggcggactt tcttgcagct
     1201 gggtggtcag ttgccactgg gcgaactcgg tcgcagcgcc aa
//
LOCUS       DAOSMQ010000677         1229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_113722_length_1229_cov_3.346678, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000677 DAOSMQ010000000
VERSION     DAOSMQ010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113722_length_1229_cov_3.346678"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1229)
                     /gene="proS"
                     /locus_tag="PK829_15320"
     CDS             complement(<1..>1229)
                     /gene="proS"
                     /locus_tag="PK829_15320"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOU42636.1"
                     /translation="TLREAPAEAELVSHQLLIRANYIRPAGAGLYTFMPLGYRVIRKI
                     WRILAEEMDAIDGQEMWMPNLLPAALWQATGRWNSFDVLFKLKGNGNREYALSATHEE
                     VVVDLALRDIESYRDLPRMVYHISKKFRDEPRPRGGLIRLREFIMKDAYTLDRSEAAL
                     DEYYPSMIQAYFNVFNRCGVPTVAINADVGAMGGKTSQEFTVPHSQGEDIYIACDNCG
                     YAANVEAAEFVREGERPAMLDTLTKVETPDCKTITDVAAFIGVPTSQTIKAVFYWWRP
                     RYVETPGEGRLVFALVRGDLAISDTKLVNALGGGFVRAATEDEIRAVGAVPGYASGVG
                     LTPAADMASPGVMVVADTSIEHGGNYVVGANEEPFHYTGANVGRDFAVSKLADIAQAD
                     TAHKCPVCGGRIEAKQA"
BASE COUNT          199 a          397 c          417 g          216 t
ORIGIN      
        1 atggcctgct tggcctcgat gcgcccgcca cagacggggc atttgtgggc cgtgtccgcc
       61 tgggcgatat cggccagctt gctgacagcg aagtcgcggc cgacgttggc cccggtgtag
      121 tggaagggtt cttcattggc cccaacgacg tagttgccac cgtgctcgat ggacgtgtcg
      181 gccacgacca tgacgcccgg cgaggccatg tcggccgccg gcgtcaggcc aacgcccgac
      241 gcatagccgg gcacggctcc cacagcgcga atctcgtcct cggtcgccgc gcggacgaag
      301 ccgccgccca gcgcgttgac cagcttggtg tcgctgatgg ccaggtcgcc gcgcaccagg
      361 gcgaagacca gccgtccctc gccgggcgtc tcgacgtagc gcggccgcca ccagtagaag
      421 acagccttga tcgtctggct ggtgggcacg ccgatgaagg cagccacatc ggtgatggtc
      481 ttgcagtccg gcgtctcgac cttggtcagg gtgtccagca tcgccggccg ttcgccctcg
      541 cgcacgaact cggccgcctc gacgttggcg gcgtagccgc agttgtcgca ggcgatatag
      601 atatcctcgc cctgcgagtg gggcacggtg aactcctgcg aagtcttgcc gcccatcgcg
      661 cccacgtcgg cgttgatggc caccgtgggc acgccacagc ggttgaagac gttgaaatag
      721 gcctggatca tgctggggta gtattcgtcc agcgccgcct cgctgcggtc gagcgtgtag
      781 gcgtccttca tgatgaactc gcgcaggcgg atgaggccac cgcgcgggcg cggctcgtcg
      841 cggaacttct tgctgatgtg gtagaccatc cggggcagat cgcggtagct ctcgatgtcg
      901 cgcagggcca gatcgacaac gacctcctcg tgcgtggccg aaagggcata ttcgcggttg
      961 ccgttgcctt tcagcttgaa gagcacatcg aacgaattcc agcggccggt ggcctgccac
     1021 agggccgccg gaagcaggtt gggcatccac atctcctgcc cgtcaatggc gtccatctcc
     1081 tcggccagga tgcgccagat cttgcggatg acgcggtagc cgaggggcat gaaggtatac
     1141 aggcccgcgc cggccggccg gatgtagttg gcacgaataa gaagttgatg gctgacaagc
     1201 tcggcctccg ccggagcctc gcgcagggt
//
LOCUS       DAOSMQ010000678         1221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_114772_length_1221_cov_5.078902, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000678 DAOSMQ010000000
VERSION     DAOSMQ010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114772_length_1221_cov_5.078902"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PK829_15325"
     CDS             complement(<1..650)
                     /locus_tag="PK829_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU42637.1"
                     /translation="MAATNRPPGALGEVARLFLRLGLTAFGGPAAHVAMFRDETVTRR
                     RWLTDAEFLDLLAATNLIPGPNSTEMAIHLGYLRAGWAGLVVGGAAFILPAFLIVLAL
                     AAVYARYGATPTAQALLYGIQPVVIAIILQAITGLGRTALRRPLPVVVAVAAFGLSFA
                     NVHELLLLAGGGLVVMLIRNGRRLWGDLPNRPTMLLLGLWGDLPNRPTFLPPKLWG"
     gene            complement(650..1039)
                     /locus_tag="PK829_15330"
     CDS             complement(650..1039)
                     /locus_tag="PK829_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU42638.1"
                     /translation="MITAVHTLIYSDDPEATRGFLRDVLRWPWVSSSDDEPEWLIFKT
                     GPSELGVHPTGGANHDGSAYQMPRKHEITLMCDDLAATMAELAARGATFSGEPMAQGF
                     GLTILLQVPGADDLLLYEPRHQLAHTL"
BASE COUNT          228 a          394 c          412 g          187 t
ORIGIN      
        1 tcgccccaca gtttcggcgg caagaaggtc gggcgatttg gcaaatcgcc ccacaatccc
       61 agcagcaaca ttgtagggcg atttggcaaa tcgccccaca gccgccggcc gttacggatc
      121 agcatgacca ccaacccgcc gccggccagc aacagcagtt cgtggacgtt ggcaaaagag
      181 aggccaaacg cggccacggc caccaccacc ggcagcggcc gccgcagcgc cgtccgcccc
      241 aggccggtga tggcctgcaa gatgatggcg atgaccaccg gctggatgcc atagagcagc
      301 gcctgcgccg ttggcgtcgc gccatagcgg gcgtagacgg ccgccaacgc cagcacgata
      361 aggaacgccg gcaggatgaa cgccgcgccg ccgacgacca gcccggccca accggcgcgc
      421 aagtagccga gatggatggc catctccgtc gagttggggc cagggatgag gttggtggcc
      481 gccagcaggt cgaggaactc ggcgtcggtc agccagcggc ggcgggtcac ggtctcatcg
      541 cggaacatgg ccacgtgggc cgccgggccg ccgaacgccg tcaggcccag gcgcaggaag
      601 aggcgagcga cttcgcccag cgcgccgggc ggccggttcg ttgccgccac tagagtgtgt
      661 gggccagctg gtggcgcggt tcgtagagca ggaggtcatc cgcgccgggg acttgtagca
      721 ggatggtcag gccgaagccc tgagccatcg gttcgccgct gaacgtcgct ccgcgcgcgg
      781 ccagctcggc catcgtcgcg gccagatcgt cgcacatcag ggtaatctca tgcttgcggg
      841 gcatctggta ggcgctgccg tcgtggtttg cgccgcccgt cgggtggacg cccagctcgc
      901 tcggccccgt cttgaagatg agccactccg gctcgtcgtc ggaactgctg acccacggcc
      961 agcggagaac atcgcgcagg aagccccgcg tagcctcggg gtcgtcggaa tagatgaggg
     1021 tgtgaacggc agtaatcatg agatagattg tacaccggtg cgtcgccgtt tctcaaatga
     1081 gcggcgcgtc cggttagggg tttttcagtg gtcggtcttc gcgcgcgcct cgacacacag
     1141 ccgtagcgca gcctgtagca cagcctgtag cacagcctgt taggacatta acaaattaac
     1201 aggcatttat gatacacttc c
//
LOCUS       DAOSMQ010000679         1197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_118264_length_1197_cov_4.129597, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000679 DAOSMQ010000000
VERSION     DAOSMQ010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118264_length_1197_cov_4.129597"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK829_15335"
     CDS             complement(<1..958)
                     /locus_tag="PK829_15335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42639.1"
                     /translation="MKASIVATVKNEGEALRPLLDSLLDQTRPPDEVVIADGGSDDAT
                     LTILDEYRALLPLRVIVAPGSNISQGRNRAIEAATGEIIAATDAGVVLSPTWLEALLA
                     PFADESIHVVSGWFEADPFTDFEVVMGATVLPSRSDIDPQSFLPSSRSVAFRRSAWAA
                     VGGYPEWLEHSEDLVFDRALRDRFGPFPFAPGAVAYFRPRSSLYAFYRQYYAYARGDG
                     KANLWPRRHAVRYVTYLLGLPLLLRAIWRERWFGWLLLFLGVGAYSRRPAERLWNNTW
                     GWRPPARARAFALIPIIRLVGDVAKMVGYPSGVWWRLRHGAPR"
BASE COUNT          220 a          386 c          437 g          154 t
ORIGIN      
        1 agcgcggcgc gccgtggcgc agccgccacc agacgccgga cgggtagcca accatcttgg
       61 ccacgtcgcc gaccagccgg atgataggga tgagggcgaa ggcgcgcgcg cgggccggcg
      121 gccgccagcc ccaggtgttg ttccacagcc gctcggccgg ccggcgcgag taggcgccga
      181 cgcccagaaa gagcaacagc cagccgaacc agcgttcgcg ccagatggct cgcagtagca
      241 ggggcaggcc gagcaagtag gtgacatagc ggacggcgtg gcggcggggc cagaggttgg
      301 ccttgccgtc gccgcgggcg taggcgtagt attggcgata gaaggcgtac aggctggagc
      361 gcggccggaa gtaggctacc gcccccggcg cgaatggaaa tgggccgaac cggtcgcgca
      421 gcgccctgtc gaagacaagg tcctcgctgt gctctagcca ctctggatag ccaccgacgg
      481 ccgcccacgc gctccggcga aaggcgaccg agcggctgga gggcaggaac gattgcgggt
      541 cgatgtcgct gcgcgagggc agcaccgtcg cgcccatgac gacctcgaag tcggtgaacg
      601 ggtcggcctc gaaccagccg ctgacgacgt gaatgctctc gtcggcaaag ggggccagca
      661 gcgcctccag ccacgtaggc gagaggacaa cgccagcatc ggtggcggcg ataatttcgc
      721 cggtggccgc ctcaatggcc cggttgcgcc cctggctgat gttgctgccc ggcgcgacga
      781 tgacacgtag cggcagcagg gcgcgatact cgtccaggat cgtcagggtg gcgtcgtccg
      841 agccgccgtc agcgatgacg acctcatcgg gcggacgggt ctggtcgagg agcgagtcga
      901 gcaagggccg cagcgcctcc ccctcgttct tgacggtagc gacgatcgat gccttcatgt
      961 tacactctag ggaaaggaac actgcatggc gcgattcgtt gcacccgcgg acgcgaaata
     1021 gcgcgctgcg tcagaggatc gcgccgtcgt aggcgaagag cctcgtggcc caaggagaac
     1081 ccccgatgga tgtcagccgc gccctggaaa tgctgttgca cggcaaccaa ctgaagcgca
     1141 cggcgcgcac cggctgggcg caacggggcg tgcccgcgcc ggaaaacgtg gccgccc
//
LOCUS       DAOSMQ010000680         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_118547_length_1195_cov_4.529825, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000680 DAOSMQ010000000
VERSION     DAOSMQ010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118547_length_1195_cov_4.529825"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PK829_15340"
     CDS             <1..898
                     /locus_tag="PK829_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42640.1"
                     /translation="TEIHHVEVQVLGDLHGRLLHLYERDCSVQRRHQKIIEESPAPVV
                     RDPALREAIAASAVRLAAAAGYTNAGTVEFIVDGAGGFYFLEMNTRLQVEHPITEAVT
                     GIDLATWQLRVAAGEPLPFAQSDIRQRGHAIECRVYAEDPAAGFLPSIGEIGYYARPS
                     GPGIRVDDGLETGGTVSPYYDPMLAKVITWGQDRPEAIRKMDRALREMVVLGVTTNID
                     YLRAILAAPAFAAGDTSTNFLAEHLAGWRPSAEVSAAEWVAAAAWEALAATRAADAPP
                     AAGGARVYDPWQTQAGWRNAGS"
BASE COUNT          171 a          467 c          389 g          168 t
ORIGIN      
        1 caccgagatc caccacgtcg aggtgcaggt gctgggcgac ctccacggcc gtctgctgca
       61 tctctatgag cgcgactgct cggtgcagcg ccgccaccag aagatcatcg aggaaagccc
      121 cgcgcccgtc gtccgcgacc cggccctgcg cgaggccatc gccgcgtcgg ccgtgcgcct
      181 ggccgcggcc gccggctata ccaacgccgg cacggtcgag ttcatcgtcg atggcgcggg
      241 cggcttctac ttcctggaga tgaacacgcg gctccaggta gaacacccca tcaccgaagc
      301 cgtcaccggc atcgacctgg ccacttggca attgcgcgtc gccgccgggg agccgttgcc
      361 cttcgcccag agcgacatcc gccagcgcgg ccacgccatc gagtgccgcg tctacgccga
      421 agacccggcc gccggcttcc tgccgtccat cggcgagatc ggctactacg cccggccgtc
      481 cggccccggc atccgcgtcg atgacggcct ggagaccggc gggaccgtct cgccctacta
      541 cgacccgatg ctggccaagg tcatcacctg ggggcaagac cggccggagg ccatccgcaa
      601 gatggaccgc gccctgcggg agatggtcgt gctgggcgtg acgaccaaca tcgactacct
      661 gcgcgccatc ctggccgcgc ccgccttcgc cgccggcgac accagcacga acttcctggc
      721 cgagcacctg gccggctggc ggccctcggc cgaggtcagc gccgccgagt gggtcgccgc
      781 cgcagcgtgg gaggcgctgg cggccacgcg cgccgcggat gcgccgccag cggccggcgg
      841 cgcgcgcgtc tatgacccct ggcagacgca ggccggttgg cgcaacgcgg ggagttgaga
      901 ggcgggggag ctggggggca ggggagctgg ggagaaaggc cccccccgct gagtccgcat
      961 cctcagatgc gggcgacttc cactcccctg ctccccgcat ccccgctccc ctgccccccg
     1021 cacccctgct cccctgctcc cctgccctcc gcacccccgc ccccctgctt ctgcacggct
     1081 tttcagttgt caccaattga cttgcgaccc gaccagcggc atgctgggaa tggcgcggtg
     1141 cctgtagcac agcctgtcag gctgtcgtcg ctggctacag cctaacaggc gtcac
//
LOCUS       DAOSMQ010000681         1194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_118717_length_1194_cov_3.359965, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000681 DAOSMQ010000000
VERSION     DAOSMQ010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118717_length_1194_cov_3.359965"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1194)
                     /gene="atpA"
                     /locus_tag="PK829_15345"
     CDS             complement(<1..>1194)
                     /gene="atpA"
                     /locus_tag="PK829_15345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOU42641.1"
                     /translation="IDGKGPVATENYRPTESRAPGVIERQPVKEPLQTGLKAIDSMIP
                     IGRGQRELIIGDRQTGKTAIALDTILNQKGQNLLCIYVAIGQRAGQVAQVVNTLEKYG
                     AMEYTTVVLAAASDPAPLQYFAPYSGCAIGEEFMEQGLDALVVYDDLSKHAWAYRQMS
                     LILRRPPGREAYPGDIFSLHSTLLERSVRLRDEFIIVPKGTKVAAETQGVNGEVYYGN
                     LVDAAIHQGMDALGEDAQDKYEVAKRPGTGGSLTALPIIETLLGDVSAYIPTNVISIT
                     DGQLFLETDLFNAGQRPAINAGLSVSRVGSAAQKRAMSKAASTLKGDLSQFRELAAFA
                     QFGSDLDPATQRQLACGERLMELLKQPQYQPIRLDHEVFMIYAGTRGYLDNVDVKQIQ
                     RWKSEF"
BASE COUNT          175 a          359 c          439 g          221 t
ORIGIN      
        1 gaattcactc ttccagcgct ggatctgctt cacgtcaacg ttatcaagat agccgcgtgt
       61 gccggcgtag atcatgaaga cctcgtggtc gaggcgaatg ggctggtact gcggctgctt
      121 gagcagctcc atcagccgct cgccgcaggc cagctgccgc tgcgtggccg ggtccaggtc
      181 agagccgaat tgagcgaagg cggccagttc gcggaactgg gacaggtcgc ccttcagggt
      241 gctggcggcc ttgctcatgg cccgcttctg ggcggcgctg ccgacgcgcg agacggaaag
      301 accggcgttg atggccgggc gctggccggc gttgaacagg tcggtctcca aaaagagctg
      361 gccgtcggtg atggagatga cgttggtcgg gatgtaggcc gacacgtcgc ccagaagggt
      421 ctcgatgatg ggtagggcgg tcagcgagcc gccggtgccg ggccgcttgg cgacctcgta
      481 cttgtcctgg gcgtcctcgc ccagcgcatc catgccctgg tggatggccg cgtcgacgag
      541 gttgccgtag tagacctcgc cgttgacgcc ctgcgtctcg gccgccacct tcgtgccctt
      601 gggcacgatg atgaattcgt cgcgcaggcg gacggaacgc tccaacaggg tgctgtgcag
      661 cgagaagatg tcgccggggt aagcctcgcg gccgggcggg cggcgcagga tgagcgacat
      721 ctgccggtag gcccaggcgt gcttggacag gtcgtcgtag accaccaggg cgtcgaggcc
      781 ctgctccatg aactcctcgc cgatggcgca gccggagtag ggggcgaagt attgcagcgg
      841 cgcggggtca gacgcggcgg ccaggacgac ggtggtgtat tccatcgcgc cgtacttctc
      901 cagcgtgttg acgacctggg cgacctgtcc ggcgcgctgg ccgatggcga cgtagatgca
      961 cagcaggttc tggcccttct ggttgaggat cgtgtccagg gcgatggctg tcttgcccgt
     1021 ctggcggtcg ccgatgatca gctcgcgctg gccgcggccg atggggatca tcgagtcgat
     1081 ggccttcaac ccggtctgca gcggctcctt caccggctgg cgctcgatga cgccgggcgc
     1141 gcgcgattcg gtgggtcggt agttctccgt ggcgacgggg cctttgccgt cgat
//
LOCUS       DAOSMQ010000682         1190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_119295_length_1190_cov_3.159471, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000682 DAOSMQ010000000
VERSION     DAOSMQ010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119295_length_1190_cov_3.159471"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PK829_15350"
     CDS             complement(<1..764)
                     /locus_tag="PK829_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42642.1"
                     /translation="MHQRYLITVQPVEATLPPPLSPDDGCRVAFMVGKSRVIIDTPDG
                     PPTPALRVATHELPAHLVITEGNIVAMDTPAWPQSGAYNQLIVSGDGDIRLHNDTFGL
                     RACFFAQDDGLLRVSNSLRLLRAAAGLAWDELGIAQMYLFGGYTATDRTVLRGAQRVA
                     AGTEYRFRVGQKEPPAAVRLARTWTTVIDDRRDHIVETICDLWTTAAARHIDPIDTPI
                     GIMLSGGLDSRLVAGAVASRGKRIIGLTFGDANSDEV"
BASE COUNT          200 a          367 c          416 g          207 t
ORIGIN      
        1 acttcgtcgc tgttcgcgtc gccaaaggtc aggccgatga tgcgcttgcc acgcgaggcc
       61 accgccccgg ccaccaggcg cgagtccagc ccgccgctga gcatgatgcc gatgggcgtg
      121 tcgatcgggt cgatgtgacg ggccgcggct gtcgtccaca ggtcacagat ggtctcaacg
      181 atgtggtcgc ggcggtcatc gataaccgtc gtccacgtgc gggccaggcg aacggccgcc
      241 ggcggctcct tctggccgac gcggaagcga tactccgtgc ccgcggcgac gcgctgtgcc
      301 ccgcgcagga cagtgcggtc ggtagccgtg taaccgccaa agaggtacat ctgggcaatg
      361 cccagctcgt cccaggccaa cccggccgcg gcccgcagca gccgcagcga gttgctgacg
      421 cgcagaaggc cgtcgtcctg ggcgaagaag caggcccgca ggccaaaagt gtcgttgtgc
      481 aggcgaatgt cgccgtcgcc gctgacgatg agttggttat aggcccccga ctgcggccag
      541 gcgggcgtgt ccatggcgac gatgttgccc tcagtgatga ccagatgggc gggcaactcg
      601 tgggtggcca cgcgcagcgc cggggtgggc gggccgtcgg gcgtgtcgat gatgacccgg
      661 ctcttgccaa ccatgaaggc cacgcggcag ccgtcgtctg gcgacagcgg cggtggcaga
      721 gtggcctcga ccggctgaac ggtgatgaga tagcgctgat gcatcctgat acctccccgt
      781 gcgtctgccc gcagcggttt gagaccacgc ggctagagcc tatgctacac gggcgcgaga
      841 gcgatcacca tagatggaac gtgaaaagat cggccgcggc gcgggaaaat aagcgatctg
      901 ctctctcact tgccgcgcgg gccatttgtt gacaaactta ccggcgtttg tcatcctcgc
      961 cccagagaag cgagtgctgt gtgtagcaca ggtcgttgac ctgttctgcg ggcagggctt
     1021 ttccagagtc gtctggcgac cattgacctg acaggtgggc ggccagtcgt tctctggtga
     1081 aaggcccgtg cgtggccgcc cacctgtcag gtcggcgcgc cgggggtgac tttggcaacg
     1141 acctgttcta cgggcaatga tgttgtgtcg gggacaggtc aacgacctgt
//
LOCUS       DAOSMQ010000683         1184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_120210_length_1184_cov_5.215235, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000683 DAOSMQ010000000
VERSION     DAOSMQ010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120210_length_1184_cov_5.215235"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1052)
                     /locus_tag="PK829_15355"
     CDS             complement(18..1052)
                     /locus_tag="PK829_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU42643.1"
                     /translation="MNYTFLGHSGLRVSALCLGTMTFGEDWGWGAPREDARTMFDAYA
                     AAGGNFIDTSSNYTNGTAERFVGEFIAADRDYWVVATKYTLRRPGSNPADLNAGGNAR
                     KNMLRTVEQSLGRLGTDHIDLLYLHMWDYSTRAEEVLRGLDDLVSAGKVLYVGISDSP
                     AWVVSRAQAIAELRGWASFVAYQAAYSIVTRGAEADVLPMTRALGLSVLAFDLLGGGV
                     LTGKFNRPGGPGEPTRESAISPEDAAAAEVILRVAAEAGRSPAQVAINWAARRSTNII
                     PILGARRPAQLDDNLGALDFDLSAEQMAALNDVRPLAREYPHTFWNDGIRRNLIYGRA
                     VDELRTDRWP"
BASE COUNT          169 a          386 c          443 g          186 t
ORIGIN      
        1 cccctatccc ctatccccta cggccagcgg tccgtccgca gttcatcgac ggcgcggccg
       61 tagatcaggt tgcggcggat gccgtcgttc caaaaggtat gggggtactc gcgggccagc
      121 ggcctgacgt cgttcagcgc ggccatctgc tcggcactca ggtcgaagtc gagcgcgccg
      181 aggttgtcgt ccagttgggc cgggcggcgc gcgccgagga tgggaatgat gttggtgctg
      241 cggcgcgccg cccagttgat ggccacctgg gccggcgagc ggccggcctc cgcggctacg
      301 cgcaggatga cctcggccgc ggcggcgtcc tccgggctga tggcgctctc gcgcgtcggc
      361 tcgccggggc cgccgggccg gttgaacttg ccggtcagca ccccgccgcc gagcaggtca
      421 aaggccagca ccgacaggcc cagggcgcgg gtcatgggca gcacgtcggc ctccgcgccg
      481 cgggtgacga tgctgtaggc ggcctggtag gccacgaacg aggcccagcc gcgcaactcg
      541 gcgatggcct gcgcccgcga gacgacccag gcgggcgagt cgctgatgcc gacgtagagc
      601 accttgccgg cgctgaccag atcgtccagc ccgcgcagca cttcctcggc gcgggtgctg
      661 tagtcccaca tgtgcaggta gagcaggtcg atgtggtcgg tgccgaggcg gccgagcgac
      721 tgctcaacgg tgcgcagcat gttcttgcgg gcgttgccgc cggcgttgag gtcggccggg
      781 ttgctgccgg ggcggcgcaa ggtgtacttg gtggccacga cccaatagtc gcggtcggcg
      841 gcgatgaact cgccgacgaa gcgctcggcc gtgccgttgg tgtagttact ggacgtgtcg
      901 atgaagttgc caccggcggc ggcataggcg tcgaacattg tccgggcgtc ttcgcggggc
      961 gcgccccacc cccagtcctc gccgaaggtc atggtgccga ggcacagcgc cgacacgcgc
     1021 aggccggagt ggccgaggaa ggtgtagttc atgggcagct ccttatggta gtgtgggtct
     1081 gtctggagag tagcccaacg gccggcgacg cgcaaagaat gaccgggctt ttccaaagtt
     1141 ccccggcgcg ccgacctgac tgacctgaca ggtgggcggc cgcg
//
LOCUS       DAOSMQ010000684         1183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_120386_length_1183_cov_3.108156, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000684 DAOSMQ010000000
VERSION     DAOSMQ010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120386_length_1183_cov_3.108156"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PK829_15360"
     CDS             <1..340
                     /locus_tag="PK829_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOU42644.1"
                     /translation="PHPFAKTRDRKLNPLQQITYVMVLNVLLPAQIITGALMWGAQRW
                     PDAAAALGGLPWLGPLHTLVAWSFAAFIILHVYLTTTGPTPTANIRAMIGGYEDVEAH
                     ETPPAAATAD"
     gene            355..954
                     /locus_tag="PK829_15365"
     CDS             355..954
                     /locus_tag="PK829_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOU42645.1"
                     /translation="MTSIPTPTPTPQASEPPFADAPAARAATRRVARPYAHPYAGGIL
                     LGLVLFATFFLTGNGLGASGGMNRLVVWLEDIFASGHVDRTAYLTTMAGGETNPFDSW
                     VVFVFIGLFIGGALSGWLFGRTKVETQRGPHTPVRVRWLFAFLGGSLMGYGARMARGC
                     TSGQALSGGAVLSVGSWAFMFSVFAGGYALAYFVRRLWN"
     gene            1000..>1183
                     /locus_tag="PK829_15370"
     CDS             1000..>1183
                     /locus_tag="PK829_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOU42646.1"
                     /translation="MAPFPLPLAELMGHWGQYVVYLLIGIAFGFALEMSGFANSKILA
                     AQFYFRDMTVLKVMFGA"
BASE COUNT          168 a          418 c          387 g          210 t
ORIGIN      
        1 gccgcacccg ttcgcgaaga cgcgcgaccg caagctgaac ccgctgcaac agatcaccta
       61 cgtgatggtg ctgaatgtgc tgctgccggc gcagatcatc accggggcgc tcatgtgggg
      121 cgcgcaacgc tggccggacg cggccgcggc gctgggcggg ctgccctggc tggggccact
      181 gcacacgctg gtggcctggt cctttgccgc cttcatcatc ctccacgtct acctgacgac
      241 caccggcccc acgcccaccg cgaacatccg ggcgatgatc ggtggctatg aggacgtgga
      301 ggcccacgag acgccaccgg ccgcggcgac ggccgattag caaacgagga gacgatgaca
      361 tccataccca cacccacacc cacaccgcaa gccagcgaac ccccgttcgc cgacgcgccg
      421 gccgcccgcg cggccacgcg gcgcgtcgcc cggccctacg cccaccccta cgcgggcggc
      481 atcttgctgg ggctggtgtt gttcgcaacc ttcttcctga ccggcaacgg cctgggcgcg
      541 tccggtggca tgaaccggct ggtcgtctgg ctggaggaca tcttcgcctc cggtcacgtc
      601 gaccgcaccg cctatctgac gacgatggcc ggcggcgaga cgaacccgtt cgatagctgg
      661 gtggtgttcg tcttcatcgg cctgttcatc ggcggggcgc tgtcgggctg gctgttcggc
      721 cggacgaaag tggagacgca acgcggcccg cacacgccgg tgcgcgtgcg ctggctgttc
      781 gccttcctgg gcggcagcct gatgggctat ggcgcgcgca tggcccgcgg ctgcaccagc
      841 ggccaggcgc tgtcgggcgg cgcggtgctg tccgtcggca gctgggcgtt catgttctct
      901 gtctttgccg gtggctacgc cctggcctac tttgtgcgcc ggctctggaa ctagcgtccg
      961 ccaggccggt cccccggccc atacgtatgg aggaatgaca tggcgccctt tccactccct
     1021 ctggccgaac tcatgggcca ctggggccag tatgttgtct acctgctgat cggcatcgcc
     1081 ttcggcttcg ccctggagat gtcgggtttc gccaacagca agattcttgc cgcgcagttc
     1141 tacttccgcg acatgaccgt gctcaaggtc atgttcgggg cca
//
LOCUS       DAOSMQ010000685         1169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_122539_length_1169_cov_5.140036, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000685 DAOSMQ010000000
VERSION     DAOSMQ010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122539_length_1169_cov_5.140036"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..>1169)
                     /gene="hutU"
                     /locus_tag="PK829_15375"
     CDS             complement(143..>1169)
                     /gene="hutU"
                     /locus_tag="PK829_15375"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOU42647.1"
                     /translation="VSEDVEDALRRVRHYTAAGEARSIGLIGNAAAVFPDLLARGLVP
                     DLVTDQTSAHDYLAYFPDDLSFEDGRALRERDPAAFARLSGAAMARQCAAMVAFQRRG
                     AIVFDYGNNLRQRAYDAGVTDAFSYPGFVPAYVRPLFCEGKGPFRWVALSGDPEDIRA
                     TDEAVLELFPDDAHLRRWIRMAQERVQFQGLPARICWLGYGERARAGLRFNELVASGA
                     VKAPIVIGRDHLDAGSVASPNRETEGMRDGSDAIADWPVLNALINAVSGATWVSYHHG
                     GGVGIGYSLHAGQVIVADGTPAAARRLERVLTVDPGMGVARHVDAGYPRAIEVARERG
                     VKIPMME"
BASE COUNT          166 a          410 c          427 g          166 t
ORIGIN      
        1 ccaactacgc tgccggggtc attcgccagc gcattgatga ggcgctggcc gggacctaga
       61 cttccgcgtc agggacgagg ggacactgac cactcgttca gaataggcaa gttcccagtt
      121 gtaccgcctc cgacagtcgc cgtcattcca tcatcggtat cttcaccccc ctctccctgg
      181 ccacctctat cgcccggggg tagcccgcgt ccacgtggcg ggcgacgccc atgccggggt
      241 caaccgtcag cacccgctcc agacggcggg cggccgccgg cgtgccgtcg gcgacgatga
      301 cctgcccggc gtgcaggctg tagccgatgc ccacgccgcc gccgtggtgg tagctgaccc
      361 acgtcgcgcc gctgacggcg ttgatgagcg cgttcagcac cggccagtcg gcgatggcgt
      421 cgctgccgtc gcgcatcccc tccgtctcgc ggttggggct ggccacggaa ccggcgtcga
      481 ggtggtcgcg gccgatgacg atgggggcct tgaccgcgcc gctggccacc agctcattaa
      541 agcgcagccc ggcgcgggcg cgctcgccat agcccagcca gcagatgcgc gccggcaagc
      601 cctggaactg cacccgctcc tgggccatgc gaatccagcg ccgcaggtgg gcgtcgtcgg
      661 gaaatagctc cagcacggcc tcgtcggtgg cgcggatgtc ttccgggtcg ccgctgaggg
      721 ccacccagcg gaacggcccc ttgccctcgc agaacagcgg ccggacgtag gcgggcacga
      781 agccggggta actgaaggcg tcggtcacgc cggcgtcgta ggcgcgctgg cgcaggttgt
      841 tgccgtagtc gaagacgatg gctccgcgcc gctggaacgc caccatcgcc gcgcactggc
      901 gggccatggc cgccccggac agccgggcga aggcggccgg gtcgcgctcg cgcagggcgc
      961 ggccgtcctc gaagctgagg tcgtcgggga agtaggccag gtagtcgtgg gccgaggtct
     1021 ggtcggtgac gaggtcgggc acgaggccgc gggccagcag gtcggggaag acggccgcgg
     1081 cgttgccgat caggccgatg gagcgggcct cgccggcggc ggtgtagtgc cgcacgcggc
     1141 gcagagcgtc ttccacgtcc tcgctgacc
//
LOCUS       DAOSMQ010000686         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_123716_length_1162_cov_4.458898, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000686 DAOSMQ010000000
VERSION     DAOSMQ010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123716_length_1162_cov_4.458898"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PK829_15380"
     CDS             <1..527
                     /locus_tag="PK829_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42648.1"
                     /translation="AGEDKAAGRPRRGLWAALLVAYLAQALVTGAFLGGRAAEPSTPA
                     PVWRVPAGATALDYQLGDAVALRGYSVTPAAGGDVLTLYWQALDFPRAEFSAFVHALD
                     ANGQTISQSDGPPAAVPMWCWVPGEVVADARRLEGDTPAAGFAVGLYDPVSGRRLAVQ
                     PATPDDRIVLPGAE"
     gene            complement(457..>1162)
                     /locus_tag="PK829_15385"
     CDS             complement(457..>1162)
                     /locus_tag="PK829_15385"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOU42649.1"
                     /translation="VAVKYYVAIVAIAFLLRREWRLLLALAVAFLVLWLVVPTVALGP
                     AGNAAFYATVRERAAHAMTTWVTEDINAQYLPSVAGRWLGTAAGRPLWRLLGYGLFGL
                     NLLAVARLVWRRAPRDMEWAFALLFLGLPLAIDTSWPHYFVYLPFAQTLVWLALREAT
                     GWTRRLVALFLVISVVLASMPFFALVGRWQDYSQYGVLFVANLGLLIAAHVLTLRPAE
                     RSGRPALPAEPPAAGR"
BASE COUNT          181 a          402 c          436 g          143 t
ORIGIN      
        1 gggcgggcga ggacaaggcg gctggccggc cacggcgcgg tctgtgggcg gcgcttctcg
       61 tggcctatct ggcgcaggcg ctcgtcaccg gcgcgtttct gggcggccgg gcggccgagc
      121 cgtctacccc ggcccccgtc tggcgcgtgc ccgccggcgc gacggctctt gactaccagc
      181 tgggcgacgc cgtggccctg cgcggctaca gcgtgactcc ggcggccggt ggcgacgtgc
      241 tgacgctcta ctggcaggcg ctcgacttcc cccgcgccga gttctccgct tttgtccacg
      301 ctctcgacgc caatggccag accatcagcc agagcgacgg cccgccggcg gccgtgccga
      361 tgtggtgctg ggttccgggc gaggtcgtgg ccgacgcgcg ccgcctggag ggcgatacgc
      421 cggcggccgg gttcgctgtc ggcctctacg acccggtcag cggccggcgg ctggcggttc
      481 agccggcaac gccggacgac cggatcgttc tgccgggcgc agagtgagga cgtgggcggc
      541 gatgagcagg ccgaggttgg ccacgaagag gacgccgtac tggctgtagt cctgccagcg
      601 gccgacgagg gcgaagaagg gcatcgacgc caggacgacc gagatgacca ggaacagagc
      661 taccagccgg cgcgtccagc cggttgcctc gcgtagcgcc agccagacga gcgtctgggc
      721 gaaaggcagg tagacgaagt agtggggcca ggaggtgtcg atggccagcg gcaggcccag
      781 aaagagcagc gcgaaggccc attccatgtc ccgcggcgcg cgccgccaga ccagccgggc
      841 cacggccagc agattcagcc caaacagccc gtagccgagc agccgccaca ggggtcgccc
      901 cgcggccgtg cccagccagc ggccggcaac gctgggcaga tattgggcgt tgatgtcctc
      961 tgtcacccag gtggtcatgg cgtgggcggc gcgctcgcgc actgtggcgt agaaggccgc
     1021 gttgccggcc ggccccagcg ccaccgtggg cacgaccagc cacaggacga ggaaggccac
     1081 ggccagcgcc agcaacagcc gccactcgcg ccgcagcagg aaagcgatgg cgacaatggc
     1141 cacgtagtac ttgaccgcca cg
//
LOCUS       DAOSMQ010000687         1161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_123851_length_1161_cov_5.137432, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000687 DAOSMQ010000000
VERSION     DAOSMQ010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123851_length_1161_cov_5.137432"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK829_15390"
     CDS             <1..416
                     /locus_tag="PK829_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU42650.1"
                     /translation="AHRWRGQNALVVREARADGRTVLFSSHVLPEVQAVCDRVGIIRA
                     GQIVATERVEQLLKSRLQRLHLRFAQMPPAGALDGDGVTELGRVGDRITFEVRANLNR
                     LLAQAVAYDVLELDNDQVTLEDIFMTYYSRNGGAL"
     gene            413..>1161
                     /locus_tag="PK829_15395"
     CDS             413..>1161
                     /locus_tag="PK829_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU42651.1"
                     /translation="MIDLLRHELRVRRGSFIGWALGLAFFCSIYTLIYPNLPAEVREI
                     DPRALALLESIGIQTFATYEGFILGTLFNFLPLLVGLFGVVVGAGALGGEEDDGTLEL
                     LAALPVSRVQLVLVKTLALAIGAALVILFDGLVAAGVFSLLGLDTPVTALDMFLTVQS
                     MWVMAFVFLAIGLFLGAYLPNHGVALGVALAALFTSFFGDNLAGMAPVLEPLQPFLPH
                     YHYESVIHMLTGDVAWREIGILVAIGVGFVLL"
BASE COUNT          155 a          391 c          390 g          225 t
ORIGIN      
        1 aggcgcaccg ctggcgcggc cagaacgccc tggtggtgcg cgaggcccgc gccgacggcc
       61 gcactgtcct cttctcctcc catgtcctgc cggaggtgca ggcggtctgc gaccgcgtcg
      121 gcatcatccg cgccgggcag atcgtggcca cggagcgggt tgagcaactc ctcaagagcc
      181 ggctccagcg gctgcacctc cgctttgccc agatgccgcc ggccggcgcg ctcgatggcg
      241 atggcgtcac cgagcttggc cgggtgggcg accggatcac cttcgaggtg cgcgccaatc
      301 tgaaccggtt gctggcccag gcggtggcct atgacgtgct ggaactggac aacgatcagg
      361 tgacgctgga agacatcttc atgacctact acagccgcaa cggaggcgcg ctatgattga
      421 cctgctacga cacgagttgc gcgtccggcg cggaagtttc atcggctggg ccttggggct
      481 ggccttcttc tgctccatct acaccctcat ctaccccaac ctgcccgccg aagtgcgcga
      541 gatcgacccg cgcgccctgg ccctgctcga atcgattggc atccagacgt tcgccaccta
      601 cgagggcttc attctgggga cgttgttcaa cttcctgccc ctgctggtgg gtctgttcgg
      661 cgtggtcgtc ggcgcgggcg cgctgggcgg ggaggaagac gacggcacgc tggagctgct
      721 ggcggcgctg cccgtctcgc gggtgcaact cgttctggtc aaaacgctgg cgctggccat
      781 cggcgcggcg ctggtcatcc tgttcgacgg cctggtcgcc gccggcgtct tcagcctgct
      841 ggggctggac acgcccgtca cggcgctgga catgttcctg accgtgcaga gcatgtgggt
      901 gatggccttt gtcttcctgg ccatcggcct gttcctgggc gcgtatctgc ccaaccacgg
      961 cgtggctctg ggcgtggcgc tggcggcgct gttcaccagc ttctttggcg acaacctggc
     1021 cggcatggcc cccgtactgg agccgttgca gccgttcctg ccgcactacc actacgagag
     1081 cgtcatccac atgctgaccg gcgacgtggc ctggcgggaa atcggcatcc tcgtggccat
     1141 cggcgtgggc tttgtgctgc t
//
LOCUS       DAOSMQ010000688         1152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_125364_length_1152_cov_4.441203, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000688 DAOSMQ010000000
VERSION     DAOSMQ010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125364_length_1152_cov_4.441203"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK829_15400"
     CDS             <1..419
                     /locus_tag="PK829_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU42652.1"
                     /translation="AFRFPRAVREGGHGYDFSFSGLKTAVMRQVSQYHSPATMPTADL
                     AASFQAAVVDVLVGKTAAAAETVGATAVHIAGGVSANRALRAAMRARLALPVRVPPLA
                     LCTDNAAMIGAAAHFHFIRGRRDSLDFDVVPSLQLV"
     gene            431..>1152
                     /locus_tag="PK829_15405"
     CDS             431..>1152
                     /locus_tag="PK829_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU42653.1"
                     /translation="MGSETTLLPHLDNTGLALNADARFRHVFSEGFDPILLTDHTGKI
                     LDGNRQALSFFGIDRKALLEANIRDLHHPDEPLPDVSKLTGPGASSFQSRVIAARVRD
                     NRPRQVTLTVEVRARRLMPGREGTWQWIYHDISSRVELEEMRQDLTAMLLHDLQSPLG
                     NVISTLELLRMQMETSGDAMLLELLDIASRSSGQLQRLIASLMDISRLEAGQPVGQQS
                     SVQLGQLVTEAYEIDEPIFARRG"
BASE COUNT          198 a          390 c          388 g          176 t
ORIGIN      
        1 ccgcctttcg tttcccacgg gcggtgaggg agggggggca cggctacgac ttcagcttca
       61 gcggcctgaa gacggcggtc atgcgccagg tcagccaata tcattcgccg gcgacgatgc
      121 caacggccga tctggcggcc agcttccagg cggctgtcgt cgatgtgctg gtgggcaaga
      181 cggcggcggc ggccgagaca gtgggggcga cggccgtcca catcgccggt ggcgtgtcgg
      241 ccaaccgcgc tctgcgggcg gccatgcggg cgcgactggc gttgccggtg cgcgtgccgc
      301 cactggccct gtgcaccgac aacgccgcta tgatcggtgc ggcggcccat ttccatttca
      361 tccgcggacg gcgcgacagc ctcgacttcg atgtcgtgcc cagtctgcaa ctggtctagg
      421 gaacacgagc atgggtagcg agacgacgct tctcccacat ctggacaaca ccggcctggc
      481 gctcaacgcc gacgcgcgtt ttcgccacgt cttctccgag ggcttcgacc cgatcttgtt
      541 gaccgaccac accggcaaga ttctggacgg caaccgccag gcgctgagct tcttcggcat
      601 cgaccgcaag gcgctgctgg aggccaacat ccgcgacctg caccaccccg acgagccgct
      661 gccggacgtg agcaagctga ccgggccagg agccagcagc ttccagagcc gggtcatcgc
      721 cgcgcgcgtg cgcgacaacc ggccgcggca ggtgacgctg acggtcgagg tgcgcgcccg
      781 ccgcctgatg cccggccgcg agggcacgtg gcagtggatt taccacgaca tctccagccg
      841 cgtagaactg gaggagatgc gccaggacct gacggccatg ctgctccacg acctgcaaag
      901 cccgctgggc aacgtcatct ccaccctgga actgttgcgc atgcagatgg agacgagcgg
      961 cgacgccatg ctgctggagc tactcgacat tgcctcgcgc agcagcggcc aattgcaacg
     1021 gctgatcgcc tccctgatgg acatcagccg cctggaagca ggccagccgg tggggcagca
     1081 aagcagcgtg caactgggcc agctggttac cgaggcttac gagatcgacg agccgatctt
     1141 cgcgcggcgc gg
//
LOCUS       DAOSMQ010000689         1144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_126731_length_1144_cov_4.299357, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000689 DAOSMQ010000000
VERSION     DAOSMQ010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126731_length_1144_cov_4.299357"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PK829_15410"
     CDS             complement(<1..1019)
                     /locus_tag="PK829_15410"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU42654.1"
                     /translation="MAHFTLVTAGSRGDIQPYIALGLGLRARGHTVRLATHALYANWI
                     RGYGLEFAPVEGDPLAAFQQEHGRDWVETGQRGREFLHGFHRFIDPLLHQATADTLAA
                     CAGTDMVLFSAVAFYVTYSVAEKLGLPFLQAYLQPINPTREFPSVIYPTRRKGGAIFN
                     YATHALGGYQIWLRLRPVLNAIRRERLGLRPLPLLGPFPDMFRRKVPVVHGYSPTLLP
                     KPKDWSAALFVPGFWFLDGDDNDTPPPALVNFLAGGPPPVYVGFGSMTGNDPERLTRV
                     TLEALRLSGQRAVLLSGWAGLAAGDLPPNVLRLDSAPHGWLFPRMAAIVHHGGVGTTH
                     EALRAG"
BASE COUNT          200 a          365 c          411 g          168 t
ORIGIN      
        1 ccggcgcgca gggcctcatg tgttgtgccc acgccgccgt ggtggacgat ggcggccatg
       61 cgcgggaaaa gccagccgtg gggcgcgctg tcgagccgca gcacgttagg cggcagatcg
      121 cccgcggcca gcccggccca acccgacagc agcaccgccc gctgcccact tagccgcagc
      181 gcctccagcg tcacgcgcgt cagccgctcc gggtcgttgc cggtcatgct gccgaaaccg
      241 acgtagaccg gcggcggccc gccggccaaa aagttgacca gcgccggcgg cggcgtatcg
      301 ttatcgtcgc cgtccaggaa ccagaagccg ggcacgaaca gcgccgcgct ccagtccttt
      361 ggcttaggca acagcgtcgg gctatagccg tgaacgacgg gcactttcct gcggaacatg
      421 tccgggaagg ggccgagcag gggcagcggc cgcaggccga gccgctcgcg gcggatggcg
      481 ttgagcaccg gccgcagccg cagccagatc tggtagccgc cgagcgcgtg ggtggcgtag
      541 ttgaagatcg ccccgccttt gcgccgggtc gggtagatga cgctggggaa ttcgcgcgtc
      601 gggttgatgg gctgcaagta ggcttgtagg aagggcaggc ccagcttctc ggcgacgctg
      661 taggtgacgt agaaggccac ggccgagaag agcaccatgt ccgtgccggc gcaggcggcc
      721 agcgtgtcgg ccgtggcctg gtgcagcagc gggtcgatga agcggtggaa gccgtgcagg
      781 aattcccgcc cgcgctgccc cgtctccacc caatcgcggc cgtgctcctg ctggaaggcg
      841 gccagcgggt cgccctccac cggggcgaac tccaggccat agccgcgaat ccagttcgca
      901 tagagcgcgt gggtggccag gcggacggtg tggccgcgcg cccgcaggcc caggccgaga
      961 gcgatgtagg gttggatgtc accgcgcgag ccggcagtga cgagggtgaa gtgcgccata
     1021 ctctttagaa accgagtttc tcagaagaaa ctcggtttct aggctatact aaccaccgct
     1081 tggctggtgg tcaagcgcaa gggaacatca tggacttcaa agacaatgtg gtaatcctga
     1141 ccgg
//
LOCUS       DAOSMQ010000690         1136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_128074_length_1136_cov_4.245143, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000690 DAOSMQ010000000
VERSION     DAOSMQ010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128074_length_1136_cov_4.245143"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PK829_15415"
     CDS             complement(<1..666)
                     /locus_tag="PK829_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42655.1"
                     /translation="MKKQLTQEAFDRAADFLRTQARPLERALFAHAFEDGRRTAVLAA
                     LVPYQNDDGGFGHGLEPDMRAAASSVVATTLALGILRRIGATEETSGLPAALVYLIDN
                     YDAESGRWPIISPGVEDAPHAPWWNYAESEANFRGFWANPRAAVVGYLWQYRKLVPSP
                     FVEGALRAAATDLLSYAHHMEMHDLLCFIDLVDTDGLPGELRQDMIDKLRRDAEGLSA
                     GRHQ"
     gene            complement(682..>1136)
                     /locus_tag="PK829_15420"
     CDS             complement(682..>1136)
                     /locus_tag="PK829_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU42656.1"
                     /translation="RYAQDEAALADTLRDVTEVESSYRPGPEAWSAKHILAHLIQGER
                     FNQQWIGELIGDVEGSYDGNAGNPELRLNATIHAFPTLADLVAELKRLNAETVALLRD
                     LPEEFVTRRRTFWRLARTLLDESYSHIGDHLQQMQAAVAAARAAGFGG"
BASE COUNT          161 a          381 c          409 g          185 t
ORIGIN      
        1 ttgatggcga ccagctgata gcccttcggc atcgcggcgc aatttgtcga tcatgtcctg
       61 ccgcagttcg ccgggcaggc cgtcggtgtc caccaggtcg atgaagcaca gcaggtcgtg
      121 catctccatg tggtgggcat agctcagcag atcggtagcc gcggcgcgca gcgccccctc
      181 gacgaacggc gacggcacga gcttgcggta ttgccacagg tagccgacga ccgccgcccg
      241 cgggttggcc cagaagccgc ggaagttggc ctcgctctcg gcgtagttcc accagggcgc
      301 gtggggcgcg tcctcgacgc cgggcgagat gatcggccag cggccgctct cggcgtcgta
      361 gttgtcgatc aggtagacca gcgccgccgg caggcccgat gtttcctcgg tcgcgccgat
      421 gcgccgcagg atgcccaggg cgagggtcgt ggccacgacc gacgaggccg cggcgcgcat
      481 gtccggctcc agcccgtggc cgaaaccgcc gtcgtcgttc tggtagggca ccagggcggc
      541 cagcaccgcc gtccggcggc cgtcctcgaa ggcgtgggcg aacaaggccc gttccagtgg
      601 tcgcgcctgg gtgcgcagga agtcggcggc gcggtcaaag gcctcttggg tcaactgctt
      661 cttcatggtg gctcgctccg gtcagccgcc gaagccggcg gcccgcgccg cggccacggc
      721 agcctgcatt tgctgcagat gatcgccgat gtgggaatag gactcgtcca gcagcgttcg
      781 cgccagccgc cagaaggtgc gccgccgggt gacgaactcc tccggcaggt cgcgcagcaa
      841 ggccaccgtc tcggcgttga gccgcttcag ttcggccacc aggtcggcca gcgtggggaa
      901 ggcgtggatc gtggcgttga gacgcagttc cgggttgccg gcattgccgt cgtagctgcc
      961 ctccacgtcg ccgattagct cgccgatcca ctgctgattg aagcgctcgc cctggatgag
     1021 atgggccagg atgtgcttgg cgctccacgc ctccgggccg gggcggtagg acgactccac
     1081 ctcggtcacg tcgcgcaggg tgtcggccag cgcggcctcg tcctgggcat agcggg
//
LOCUS       DAOSMQ010000691         1131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_128954_length_1131_cov_3.463755, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000691 DAOSMQ010000000
VERSION     DAOSMQ010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128954_length_1131_cov_3.463755"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1131)
                     /locus_tag="PK829_15425"
     CDS             complement(<1..>1131)
                     /locus_tag="PK829_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOU42657.1"
                     /translation="TYDGDTPRGKRAAVRSAGGILITNPDMLHAGILPFHPSWRALFG
                     ALEYVVVDEIHTYRGVFGSHVANVLRRLQRLCRFYGSDPRFICASATIANPAEHAERL
                     IEQSFTLVDESQNGAPRGAKDFILYNPPITNEELGLRASTVLTAKDAAIAFLGQDIQT
                     IVFARARQTVELLLGYLRDDLAYQNRGATFVAGYRGGYLPLERREIEAGLRHGTVRGV
                     VATNALELGIDIGQLDAAVLTGYPGTVASTWQQVGRAGRRAERSAAILVAGSGALDQY
                     LCLHPRFLFGRSPEYALTNPDNLPILTRHLLCAAYELPFTADESFGGFERVGPVLEEL
                     ARAGDLYASRDQYHYVGDAPASGISLRTGGDETVVIQDISEE"
BASE COUNT          180 a          335 c          433 g          183 t
ORIGIN      
        1 tgctcctcgc tgatgtcctg gatgaccacg gtctcgtcgc cgccggtacg cagcgagatg
       61 ccgctggccg gagcgtcgcc gacgtagtgg tactggtcgc ggctggcgta caggtcgccg
      121 gcgcgggcca gttcctccag caccggcccg acgcgctcga agccaccgaa ggactcgtcg
      181 gcggtgaagg gcagttcgta ggcggcgcag agtagatggc gggtcaggat gggcaggttg
      241 tcggggttgg tcagggcata ctccggcgag cggccgaaca ggaagcgcgg atgcaggcag
      301 aggtattggt ccagcgcgcc gctgccggcg acgaggatgg ccgcggagcg ttcggcgcga
      361 cggccggcgc ggccgacctg ctgccacgtg ctggccactg tgccgggata gccagtcagt
      421 acggcggcgt caagctggcc aatgtcgatg cccagttcca gcgcgttggt ggccacgacg
      481 ccacgcactg tgccgtggcg tagcccggcc tcgatctcgc gtcgctccag gggcagatag
      541 ccgccgcggt agccggccac gaaggtggcg ccacggttct ggtaggccag gtcgtcgcgc
      601 aaatagccga gcagcagctc aaccgtctgc cgggcgcggg cgaagacgat ggtctgaatg
      661 tcctggccca ggaaagcgat ggccgcgtct ttggccgtca gcacggtgct ggcccgtagg
      721 ccaagctcct cgttggtgat gggcggattg tagaggatga agtctttggc cccgcgcggc
      781 gcgccgttct gggactcatc gacgagcgtg aacgattgtt cgattaaacg ttcagcgtgt
      841 tcggcggggt tggcgatggt cgccgaggcg caaataaagc gcggatcgct gccatagaag
      901 cggcataggc gttgcaggcg gcgcaggacg ttggccacgt ggctgccgaa gacgccgcgg
      961 taggtgtgaa tctcgtcgac gacgacgtac tccagcgcgc cgaagagcgc ccgccacgac
     1021 gggtggaagg gcaggatgcc ggcgtggagc atgtcggggt tggtgatgag gatgccgcca
     1081 gcactgcgca cggccgcgcg tttgccgcgc ggcgtgtcgc cgtcataggt g
//
LOCUS       DAOSMQ010000692         1122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_130520_length_1122_cov_3.521087, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000692 DAOSMQ010000000
VERSION     DAOSMQ010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130520_length_1122_cov_3.521087"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PK829_15430"
     CDS             complement(<1..678)
                     /locus_tag="PK829_15430"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOU42658.1"
                     /translation="MSITLRRSLLVVAAVALAIVVLALGGRPAAAQSDNPDLWYAQFW
                     TNQELRGTPIMTRWDRTIDFRWWGGAPDPRVGDDNFSVRWTRAVPFAAGNYRFFATMD
                     DGMRVWINDQLIIDSWTRSQEHTVSVDRFIPAGTHNIRVEHFEDGGQATAIFRWELIG
                     ASNAGPQFPNWRGVYFNTVNLTGAPVLVRDDAYLNQNWGTGSPAPGIVNSDLWSARWT
                     RAVAVQPG"
     gene            complement(884..>1122)
                     /locus_tag="PK829_15435"
     CDS             complement(884..>1122)
                     /locus_tag="PK829_15435"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU42659.1"
                     /translation="LPTASQGVVISARPLNVRAAPGTGSALITQLQPCSQPLLTGNRS
                     SDGLWAEVLTDNGQIGWVLRQYLQTVGDASEGVG"
BASE COUNT          203 a          333 c          397 g          189 t
ORIGIN      
        1 gccgggctgc acagccacgg cgcgcgtcca gcgggccgac cacagatcgg agttgacaat
       61 gccgggggcg ggtgagcctg tgccccaatt ctggttgaga taggcatcgt cgcggacgag
      121 cacgggcgcg ccggtcaggt tcaccgtgtt gaagtagacg ccgcgccagt tggggaactg
      181 cgggccggcg ttgctcgcgc cgatcagctc ccagcggaag atggccgtgg cctggccgcc
      241 gtcctcgaag tgctccacgc ggatgttgtg ggtgccggcg ggaatgaagc ggtcaaccga
      301 gacggtgtgc tcctggctgc gtgtccagct atcgatgatg agctggtcgt tgatccagac
      361 gcgcatgccg tcgtccatgg tggcgaagaa gcggtagttg ccggcggcaa agggcacggc
      421 gcgggtccag cggacggaga agttgtcgtc gccgacgcgg gggtcgggcg cgccacccca
      481 ccagcggaag tcgatggtgc ggtcccaacg ggtcatgatg ggcgtgccgc gcaattcctg
      541 gttggtccag aactgggcat accagaggtc aggattgtcg ctctgggccg cggctgggcg
      601 gccgcccaga gccaatacga cgatcgccag cgcgacggcg gcgaccacca aaagactacg
      661 acggagagta atggacatac gaagttactc cttgcctaat gtggccgaac cttttttatg
      721 tggcgcacaa ctgatccatt ataatgacca caaagaaacg gtcaagtgtc gcgggcgcaa
      781 ttacccatag ccatggccag gccagagcaa gcgctcaggc cagagcgccc aggagccagg
      841 gcgctaagag aagtcttgat cagcggctgt ttttccgcgc cttctaaccg acaccttcgc
      901 tggcgtcgcc gacggtctga aggtattgcc gtagcaccca accgatctgc ccgttgtcgg
      961 tcagaacctc ggcccagagg ccgtcgctgc tgcggttgcc ggtcagcagc ggctggctgc
     1021 acggctgtag ttgggtgatg agggcgctgc ccgtgcccgg cgcggcacgg acgttgagcg
     1081 gccgggcgct gatgacgacg ccctggctgg ccgtgggcaa ga
//
LOCUS       DAOSMQ010000693         1118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_131253_length_1118_cov_3.767639, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000693 DAOSMQ010000000
VERSION     DAOSMQ010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131253_length_1118_cov_3.767639"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PK829_15440"
     CDS             complement(<1..847)
                     /locus_tag="PK829_15440"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOU42660.1"
                     /translation="MITGKTRLIGLLGWPVGHSISPAMHNAAAAALGLDWAYVPLPVR
                     PEALPSALGGLAALGFRGVNVTVPHKTAVLPYLDAVYPVAQAIGAANTIAIGDGQLTG
                     FNTDWSGFLADLEENEVDVRGRDCVVLGAGGAARAVVYALARNGARVTILARREEQAE
                     QLAGDLSEGAPVPVAAGSLARVVEVVRAARRPLLVNATPLGMAGGTYAGLSPWPDAPL
                     PADAFVYDLLYNPPLTPLLRAAQFAGCGHANGLGMLVNQAAEAFELWTSRRPDRAVMR
                     RAAEAV"
     gene            complement(844..>1118)
                     /locus_tag="PK829_15445"
     CDS             complement(844..>1118)
                     /locus_tag="PK829_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42661.1"
                     /translation="LAGRVVRLLGIFDSRRWHSQKRIHFYHAVFQIEVDDPQPRPTAE
                     STAVAYFAEDALPPLSPGHHLRAPFVFRQLRGDAPLPYFDPPEDSS"
BASE COUNT          170 a          383 c          431 g          134 t
ORIGIN      
        1 ccacggcctc ggcggcgcgg cgcatgacgg cgcggtcggg ccggcggctg gtccatagct
       61 cgaacgcctc ggcggcctga ttgaccagca tgcccagccc gttggcgtgg ccacagccgg
      121 caaactgggc ggcgcgcagc agcggcgtca gcggcgggtt gtagagcaga tcgtagacga
      181 aggcatcggc cggcagcggc gcgtcgggcc agggcgacag gccggcgtag gttccgccgg
      241 ccatgcccag cggcgtggcg ttgaccagca gcggccggcg cgcggcgcga acgacctcga
      301 cgacacgcgc cagcgacccg gcggcgacgg gcacgggcgc gccctcgctc aggtcgccgg
      361 ccagttgctc ggcctgttcc tcgcgtcggg ccaggatggt gacgcgcgcg ccgttgcgcg
      421 ccagggcata gaccacggcc cgcgctgccc cgcccgcgcc cagcaccacg caatcgcgcc
      481 cgcgcacatc gacctcgttc tcctccaggt cggccaggaa gccggaccag tcggtgttga
      541 agccggtcag ctggccgtcg ccaatggcga tggtgttggc cgcgccgatg gcctgggcga
      601 cgggatagac cgcatcgagg taaggtagga cggcggtctt gtggggcaca gtcacgttga
      661 cgccgcggaa gccgagcgcc gccagcccgc ccagcgccga gggcagcgcc tccggccgca
      721 cgggcagagg cacgtaggcc cagtcgaggc cgagggccgc cgcggcggcg ttgtgcatgg
      781 ccgggctgat gctatggccc acgggccagc cgagcaggcc gatgagtcgg gtcttgccgg
      841 tgatcatgac gaatcctcag gcgggtcgaa atagggcagc ggcgcgtcgc cgcgcaactg
      901 gcggaagacg aacggcgcgc gcaaatggtg gccgggcgag agcggcggca gcgcgtcctc
      961 ggcgaagtag gccacggccg tactctcggc cgtcggccgc ggctgcgggt catccacttc
     1021 gatctggaag acggcatgat agaagtggat gcgcttctgg ctatgccagc ggcgcgagtc
     1081 gaagatgccc agcagccgga cgacgcgccc ggccaggc
//
LOCUS       DAOSMQ010000694         1110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_132742_length_1110_cov_2.790521, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000694 DAOSMQ010000000
VERSION     DAOSMQ010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132742_length_1110_cov_2.790521"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PK829_15450"
     CDS             <1..1109
                     /locus_tag="PK829_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU42662.1"
                     /translation="PHTSPHRVAEAEQALLALVARHGVTLIAIGNGTASRETERLVAG
                     LTRERADLHYVIVSEAGASVYSASPLARAEMPHLDVSLRGAVSIGRRLQDPLAELVKI
                     DPQSIGVGLYQHDVDQKQLAGALTSVVESVVNQVGVEVNTASPALLGYVSGIGPKLAG
                     NIVAHRDANGGFPSRAALRAVAGLGAKAFEQAAGFLRIRDGENPLDASAIHPESYPVA
                     ARLLARLGLPPGTPAEELRPALQTLREKSSLDALAAELGTGAPTLGDILDQLAQPGRD
                     PRADLPLPLLRSDVVSMDDLTPGQVLTGTVRNVVDFGAFVDIGVKQDGLLHKRAMPRG
                     TALQVGDIVAVCVLNIDKERGRIGLGWPGAPPAQ"
BASE COUNT          169 a          410 c          374 g          157 t
ORIGIN      
        1 atccccacac gtcgccgcac cgcgtcgccg aggccgagca ggcgctgctg gccctcgtcg
       61 cccgccacgg cgtcaccctc atcgccattg gcaacggcac ggcctcgcgc gagacggagc
      121 gactggtggc cgggctgacg cgcgagcggg ccgatctgca ttacgtcatc gtcagcgagg
      181 ccggggccag cgtctatagc gccagcccgc tggcccgcgc cgagatgccc cacctggacg
      241 tgtcgttgcg cggcgcggtg tcgatcggcc gtcgcctgca agacccgctg gccgaactgg
      301 tcaagatcga cccgcaaagc atcggcgtgg gcctctacca gcacgacgtc gaccagaagc
      361 agctggccgg ggcgctgacg tcggtcgtcg agtcggtcgt caatcaggtc ggcgtggagg
      421 tgaacacggc ttcacccgcc ctgctcggct acgtgtccgg catcgggccg aagctggccg
      481 gcaacatcgt cgcccaccgc gatgccaacg gcggctttcc cagccgcgcc gccctgcgcg
      541 ccgtcgctgg cctgggagct aaggcattcg agcaggccgc cggcttcctg cgcatccgcg
      601 atggcgagaa tccgctggac gccagcgcca tccacccgga gagctacccg gtggcggcgc
      661 gactgctggc gcggctgggg ctgccgccgg gcacgcccgc ggaggaactg cgcccggctc
      721 tgcaaacact acgcgaaaaa tcgtccctgg atgcccttgc cgccgaactg ggcacaggcg
      781 cgcccaccct cggcgacatc cttgaccaac tggcccagcc cggccgcgac ccgcgcgccg
      841 acctacccct gcccctgctg cgcagcgatg tcgtctccat ggacgacctg acccccggcc
      901 aggtgctaac cggcaccgtg cgcaacgtcg tcgatttcgg cgcgtttgtc gatatcggtg
      961 tcaagcaaga cggcctgctg cacaagcgag ctatgccgcg cggcacggct ctgcaagtcg
     1021 gcgacattgt ggcggtctgc gtgctgaaca tcgacaagga gcgcgggcgg atcggtctgg
     1081 gctggccggg cgcgccgccg gcgcaataag
//
LOCUS       DAOSMQ010000695         1104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_133846_length_1104_cov_3.813155, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000695 DAOSMQ010000000
VERSION     DAOSMQ010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133846_length_1104_cov_3.813155"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK829_15455"
     CDS             complement(<1..307)
                     /locus_tag="PK829_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOU42663.1"
                     /translation="MHILSVNVGQRTPIANAKASGITGIYKTPAAEPARVTALGLAGD
                     FIADTEHHGGVDQAVYVYGTDDYDWWAAELGRPLAPGTFGKNLTVAGLASGELAVGDR
                     "
     gene            complement(377..1051)
                     /locus_tag="PK829_15460"
     CDS             complement(377..1051)
                     /locus_tag="PK829_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42664.1"
                     /translation="MDANETASRQALLQVLTTEHFTLQGARAVAMSEVSARASIYLSA
                     VSSAVVALALVTSLAQTEEMIRAFALVLLPIVAFMGFVTKARLIQLSRTDFHYQRAIN
                     RIRHAYVDAAPESARYFLLSIHDDVPGVVESSVFQRGRRMGVLTAAQMIGVVNYVIVG
                     LAAGIVIDWLTGATAWLSIVMGIVVGLAVALWDFRREEAQWRQYVATFDVLFPSPEEV
                     EERRAS"
BASE COUNT          195 a          350 c          378 g          181 t
ORIGIN      
        1 accggtcgcc gacggccagt tcgccgctgg ccagcccggc gacggtcagg ttcttgccga
       61 acgtgcctgg ggccagcggc cggcccaact cggccgccca ccagtcgtag tcgtctgtgc
      121 catagacgta gacggcctga tcgacgccgc cgtgatgctc cgtgtcggcg atgaagtcgc
      181 cggccaggcc gagggccgtc acccgcgccg gctcggcggc cggggtcttg tagatgccgg
      241 tgatgcccga cgccttggcg ttggcgatgg gcgtgcgttg gccgacgttg acgctgagaa
      301 tgtgcatggt gtgtctccca aataataaat aatagtgggc agctgtaggg ccggtaaggg
      361 ttgtcgcctc cgccagttag ctggcgcgcc gctcttccac ctcttccggc gacgggaaga
      421 gcacgtcgaa cgtggcgacg tattgccgcc actgggcctc ctcgcgccgg aaatcccaca
      481 gggcgacggc caggccgaca acgatgccca tcacaatcga cagccacgcc gtcgccccgg
      541 tcagccagtc aatgacgatg cccgcggcca ggccgacgat aacgtagttg acgacgccga
      601 tcatctgggc ggcggtcagc acacccatgc gccggccgcg ctgaaagacc gacgactcca
      661 ccacacccgg cacgtcatcg tgaatcgaca acaggaagta gcgggccgat tcgggggcgg
      721 cgtcgacgta ggcatggcgg atgcgattga tggcccgttg atagtggaag tcggtgcggc
      781 tgagctggat caggcgcgcc ttggtgacga agcccatgaa ggcgacaatg ggcaacagca
      841 ccagggcgaa ggcgcggatc atctcctccg tctgggccag cgacgtgacc agcgccaggg
      901 cgacgacggc gctggagacg gcgctcaggt agatgctggc ccgtgcgctg acctcggaca
      961 tggccaccgc cctggcccct tgcagggtga agtgctcggt cgtcaacacc tgaagcagtg
     1021 cttgtcgtga cgcggtttcg ttggcatcca tatggctcta tagtctttgg ccgcgattcg
     1081 ggagaatcgc gccacgggga acgg
//
LOCUS       DAOSMQ010000696         1094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_135723_length_1094_cov_4.276227, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000696 DAOSMQ010000000
VERSION     DAOSMQ010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135723_length_1094_cov_4.276227"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PK829_15465"
     CDS             <1..1006
                     /locus_tag="PK829_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOU42665.1"
                     /translation="KPSALGDIPGYGPPLLSPDGRLLALNGNWGGATALDLATGEVIG
                     PGRGRGMHRPTWSPDSRKLAYMAANDQLCVYHLDDAPADCIFGNSRLQEILWSPTSEV
                     IAAAVTDPLAADSIDCCTGQVWLVDALTGEASAIPVAYATGFEAAPNEVLAWLPDGTG
                     LVIKKTADGRGAIYHIGDGSRTDFAEPILSVSPDGQMVLHPSGAVSNVDGRARFTLPG
                     AAGCPSGAHLVYVWSPDGARLATTWVCFRTDGGSDPVAPLTVFDTAAGAPLWQQPLAP
                     DTWPVAWSPDDAYLLLQENATGLQNYDQLGDYALWQVAADGGTPELVAQGAVLLATIP
                     "
BASE COUNT          153 a          403 c          369 g          169 t
ORIGIN      
        1 caagcccagc gcgctgggcg acatccccgg ctacggcccg ccgcttctct cgcccgacgg
       61 ccggctgctg gctctcaacg gcaactgggg cggtgcgacg gcgctcgacc tggccaccgg
      121 cgaggtgatc ggccccggcc gcggccgggg gatgcaccgg cccacttggt cgcccgacag
      181 ccgcaaactg gcctacatgg ccgccaacga ccaactgtgc gtctaccacc tcgatgacgc
      241 tccggccgac tgtatctttg gcaatagccg gctccaggaa atcctgtggt cgcccacgtc
      301 ggaggtgatc gccgccgccg tcactgatcc gctggccgcg gacagcatcg actgttgcac
      361 cggtcaggtg tggctggtgg acgcgctcac cggcgaggcg agcgccatcc ctgtcgccta
      421 cgccaccggc ttcgaggccg cgcccaacga agtgctggcc tggctgcccg acggcacggg
      481 gctggtcatc aagaagacgg ccgacggccg cggggcgatc taccacatcg gcgatggttc
      541 gcgcaccgat ttcgccgagc cgatcctcag cgtctcgccc gacggccaga tggtgctgca
      601 cccgtccggc gcggtgagca acgtggacgg ccgcgcgcgc ttcacgctgc ccggcgcggc
      661 cggctgcccc agcggcgcgc atctcgtcta cgtttggtcg cccgatgggg cgcggctggc
      721 cacgacgtgg gtgtgtttca ggaccgacgg cggttccgat ccagttgcgc cactgacggt
      781 gttcgacacg gctgcgggcg cgccgctgtg gcaacagccc ctcgcgcccg acacctggcc
      841 cgtggcctgg tcgcccgacg acgcttacct gctgttgcag gaaaacgcta ccgggctgca
      901 aaactacgac cagttaggcg actatgccct ctggcaggtg gccgccgatg gcgggacgcc
      961 ggaactggtg gcccagggcg ctgtgttgct ggcgactatc ccgtaacgac tcgccccgtc
     1021 tgcccgcgcc ggccggcggc ttctccttgc ccgcaacgac gatctctatc gcgccgacct
     1081 ggccgggggc aacg
//
LOCUS       DAOSMQ010000697         1093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_135977_length_1093_cov_2.319846, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000697 DAOSMQ010000000
VERSION     DAOSMQ010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135977_length_1093_cov_2.319846"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..703
                     /locus_tag="PK829_15470"
     CDS             353..703
                     /locus_tag="PK829_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU42666.1"
                     /translation="MGLYRRLFGGGVKSISPEEAHRRLQADTEVVIVDVRQPLETRAG
                     SVPGAVLIPLTEFGRRLAELPRDRPILTICRSGHRSPIAARQLRAAGYDVTDVDGGTL
                     AWERAGLPLSPPES"
     gene            complement(709..>1093)
                     /locus_tag="PK829_15475"
     CDS             complement(709..>1093)
                     /locus_tag="PK829_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU42667.1"
                     /translation="QMMAREEFRRLPVVDKDGRLIGIVSLGDVRSAEPSPATSLSVWE
                     LNYLLSTLTIAKIMTPNPFAIGPDATLAEAARMMLEHRVSGLPVVDETRHVIGIITES
                     DIFAMVVLHEWGAEYEHEREPAEQG"
BASE COUNT          193 a          327 c          366 g          207 t
ORIGIN      
        1 gtccaccagg tcagccttaa ccacagcggc gacgagtgtg gggaagtcgg gattcgacgc
       61 ggcgatctcg acaatgtttt gccggcgcat ggcaaacgtg ggcaaggccg ctgccagcag
      121 cagggcgacg acgagaacac tatagaccac tttgtgaaca cggctcagtt tcatgctaac
      181 ctccagtaga gtgtcttgaa caacctttgt cttattcaac cgggcggtcc taacgtcccg
      241 tgttggtaat gcaggcaata taatggatta gtccatattt tgtaaaggtt atgtacgcta
      301 ctgttaactg gcgctcataa atgggccgct ggcgcggccg agagaggcaa tcatgggact
      361 ttaccggcgc ttgtttggtg gtggggtgaa aagcatcagc ccggaggagg cgcaccgacg
      421 cttgcaggcg gatacagagg tggtcatcgt cgacgtgcgc cagcccttgg agacgcgggc
      481 gggcagcgtg cccggcgcgg tgctcatccc gctgacggag ttcggccggc ggctggcaga
      541 actgccgcgc gaccggccca tcctgactat ctgtcgctcc ggccaccgca gccccatcgc
      601 cgcgcggcag ttgcgggcgg ccggctatga cgtcaccgac gtggacggcg gcacgttggc
      661 ctgggagcgg gccgggctgc cgctgtcgcc gccagaatcg tagtcgatct agccttgttc
      721 tgccggttcg cgctcgtgct cgtactcagc gccccactcg tgtagcacga ccatggcgaa
      781 gatgtcggac tcagtgatga tgccgatgac gtggcgcgtc tcgtcgacga cgggcaggcc
      841 gctgacccgg tgctccagca tcatccgcgc cgcctctgcc agcgtggcgt cggggccaat
      901 ggcgaaagga ttcggcgtca tgattttggc gatggtcagc gtcgatagca ggtagttgag
      961 ttcccagacg ctcagcgacg tggcgggcga cggctcggcg ctgcgcacgt cgcccaggct
     1021 gacgatgccg atcaggcggc cgtccttgtc cacgaccggc aggcggcgaa actcctcgcg
     1081 ggccatcatc tgg
//
LOCUS       DAOSMQ010000698         1084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_137735_length_1084_cov_2.124393, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000698 DAOSMQ010000000
VERSION     DAOSMQ010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137735_length_1084_cov_2.124393"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..451)
                     /locus_tag="PK829_15480"
     CDS             complement(278..451)
                     /locus_tag="PK829_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42668.1"
                     /translation="MDAVGAGQVADGIEDVWPAAQVGHQEDLVDALTWPRLGGVFLAG
                     QQQHARAEGLGGG"
     gene            565..>1084
                     /locus_tag="PK829_15485"
     CDS             565..>1084
                     /locus_tag="PK829_15485"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOU42669.1"
                     /translation="MRTQAAAAGIPVPPFTPVFSYDRLRAYMERVPPPWVLKPRSEAG
                     AMGIKKLYSSEEVWRWLDQLGDEQSFFHLEQFIPGEIYHVDSLVYDSRILFAQPHKYA
                     RPPMTVAHEGGVFVSRTLPDGEEARAILDLNARLLAAFGLERGATHAEFIRGADGQYY
                     FLEVAARVGGANI"
BASE COUNT          181 a          369 c          334 g          200 t
ORIGIN      
        1 tcggccgggt gaatggcgat tgccgcgtcc aggcttataa atgtacgttc ggcagtggca
       61 cgttgccgtg acacttcaat gtggcacgtt tacttatgcg ctcttgccga gtgctgccag
      121 ataagtatag caggccagtc catttcgtca acagccaggc gcgccatttc gtgatcttcg
      181 gcgcgattgc gtctatgatt gcgcccggag gcaagtcatg caacctgaac cgacgacgat
      241 cctttgtctg gccagctact ttaagggcac gaccttccta accgccgcca aggccctcgg
      301 cgcgcgcgtg ctgctgctga cccgcgagaa aaacgccgcc gagccgtggc cacgtgagag
      361 catcgacgag gtcttcctga tgcccgacct gcgccgccgg ccagacatct tctatgccgt
      421 cagctacctg gcccgcgcca accgcatcca tgccattgta ccgttgacga ctacgacgtc
      481 gagacggccg ccgccctgcg cgagcacctg cgcctgcccg gcctgggcga gacgggggcg
      541 cgctacttcc gcgacaaact ggccatgcgc acccaggccg cggccgccgg catccctgtg
      601 cctcccttta cgcccgtgtt tagctacgac cggctgcggg cctacatgga acgcgtgccg
      661 ccgccgtggg tgctgaagcc gcgctccgag gccggggcga tgggcatcaa gaagctctac
      721 tcgtcggaag aagtctggcg ctggctcgac cagttgggcg atgagcagtc gttcttccac
      781 ctggagcagt tcatccccgg cgagatctat catgtcgatt cactggtcta cgacagccgc
      841 atcctctttg cccagccgca caagtacgcc cggccgccta tgaccgtggc ccacgagggc
      901 ggcgtcttcg tctcgcgaac gctgcccgat ggcgaggaag cgcgggccat cctcgacctc
      961 aacgcgcggc tactggccgc cttcggcctg gagcgcggcg cgacccatgc cgagttcatc
     1021 cgcggggccg acgggcagta ttactttctg gaagtggccg cgcgggtggg cggagccaac
     1081 atcg
//
LOCUS       DAOSMQ010000699         1071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_140297_length_1071_cov_3.524606, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000699 DAOSMQ010000000
VERSION     DAOSMQ010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140297_length_1071_cov_3.524606"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1071
                     /locus_tag="PK829_15490"
     CDS             <1..>1071
                     /locus_tag="PK829_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HOU42670.1"
                     /translation="SYDAAAGTLTTAAADPAAGGATMPPPVVSTPPDLVVVPGTIQSV
                     LGCADDWLPDCETTALTYDADDDLWQGTFALPAGEYEYKAALNGTWDANFGLNAAAGG
                     PNIPLVVEEDADVSFFFSTQTGWITDNVNSTIATAAGSFQDEIGCPAEWSPDCLRSWL
                     QDPDGDGTFVFQTDAIPAGAYEAKVAINQTWDENYGEAGAPGGANIPFTVDADGTLVS
                     FLWNAANKIMTIQVGGSGQVGSLAEAKAHWVAADTIVWDVEYNPDNQYAFYYTETGEP
                     LVLGSDGVSGSPLLLSYVPEGLSPELAAKFPHLAGQAVFHLPARVGTLRVALKGQVAV
                     AATDAEDNLLDATALQIPGVLDD"
BASE COUNT          182 a          388 c          344 g          157 t
ORIGIN      
        1 agctatgacg ccgccgccgg cacgctgacc acggccgcgg ccgacccggc cgccggcggg
       61 gcgaccatgc cgccgccggt ggtcagcacg ccgccggacc tggtcgttgt gcccggcacc
      121 atccagtctg ttctgggctg cgccgacgac tggctgccgg attgcgagac cacggcgctg
      181 acctacgacg ccgacgatga cctctggcag ggaacgtttg ccctgccggc cggtgagtac
      241 gagtacaaag ccgccctcaa cggcacatgg gatgccaact tcggcctcaa cgccgcggcc
      301 ggcggcccca acatccccct ggtggtcgaa gaggacgccg acgtgagctt cttcttctcc
      361 acgcagaccg gctggatcac cgacaacgtc aacagcacta tcgccacagc cgcgggcagc
      421 ttccaggacg agatcggctg tccggccgag tggtcgcccg actgcctgcg ctcctggcta
      481 caagacccgg acggcgacgg cacttttgtc ttccagaccg acgccatccc cgccggggcc
      541 tacgaggcca aagtggccat caaccagacc tgggacgaga actacggcga ggccggcgcg
      601 cccggcgggg ccaacatccc cttcaccgtt gacgccgacg gcacactggt gtccttcttg
      661 tggaacgccg ccaacaaaat catgaccatc caggtcggcg gcagcggcca ggtgggtagc
      721 ctggccgagg ccaaagccca ttgggtcgcc gccgatacca tcgtctggga tgtcgagtat
      781 aacccggaca atcagtacgc cttctactac accgagacgg gcgaaccgct ggtgctcggc
      841 tcggacggcg tcagtggttc gccgctcctc ctgagctacg tgcccgaagg gctgtcgccg
      901 gaactggccg ccaaattccc ccatctggcc ggccaggcgg tctttcacct gccggcgcgg
      961 gtgggcacgc tgcgggtggc gctgaagggg caggtggccg tggccgcgac cgacgccgag
     1021 gataacctgc tcgacgccac cgccctgcag atccccggcg tgctcgacga c
//
LOCUS       DAOSMQ010000700         1061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_142387_length_1061_cov_3.944334, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000700 DAOSMQ010000000
VERSION     DAOSMQ010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142387_length_1061_cov_3.944334"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..>1061)
                     /locus_tag="PK829_15495"
     CDS             complement(29..>1061)
                     /locus_tag="PK829_15495"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOU42671.1"
                     /translation="RMQKRDVMVRQVSRTFALSIEQLPPLLREAISVAYLMFRISDGI
                     EDHEAMPAARKVVLLDEWAAVLEGRAPAAGLARRVDDLDPADPEVEVVRETPLILDWL
                     HALPAPFQEPIVAHVHATTRGMARWQAHGPYVEDEAALDDYMHEVAGRVGYLVTDLFA
                     LYSPAVAAQRESLMPLSRECGLALQTVNIVRGIRKDYERGWVFVPQTFYRSLGLTRET
                     LLALPQEPKALAVVAMLADKADRHLDHGLRYITTFPRSEHSLRLALMWPFLFAARTLA
                     LSRNNPNVLRSEAKMTRAEIGRIVAESKLMGWSNSWLARTYARLARPPAPPGQPPAIR
                     LVAAGPRDR"
BASE COUNT          171 a          333 c          392 g          165 t
ORIGIN      
        1 gcgtgcgtcg gtcatcgggg cgatctcctt agcggtcgcg cgggcctgcg gcgaccagac
       61 gaatggccgg tggttggccg ggcggggccg gcgggcgggc caggcgggcg taggtgcgtg
      121 ccagccagct gttagaccag cccatcagct tcgattcggc gacaatgcgg ccgatctcgg
      181 cgcgggtcat tttggcctcg ctgcgcagga cgttggggtt gttgcggctc agggccagcg
      241 tccgcgcggc gaagaggaaa ggccacatca gcgccagcct caggctatgc tcagaccgcg
      301 ggaaggtggt gatgtagcgc aggccgtggt cgaggtggcg gtcggccttg tcggccagca
      361 tggccacgac ggccaatgct ttcggctctt gcggcagcgc cagcagcgtc tcgcgcgtca
      421 ggcccaggct acggtagaac gtctgcggca caaagaccca gccgcgctcg tagtccttgc
      481 gaatgccgcg gacgatattg accgtttgca gcgccaggcc acactcgcgc gagaggggca
      541 tcaggctctc ccgctgcgcg gccacagccg gcgagtagag cgcaaagaga tcggtcacca
      601 ggtagccgac gcggccggcg acctcgtgca tgtaatcatc gagggcggcc tcgtcttcga
      661 cgtaggggcc gtgggcttgc cagcgggcca tgccgcgcgt ggtggcgtgg acgtgggcga
      721 caataggttc ctgaaagggc gcgggcaggg catggagcca gtcgaggatg agcggcgtct
      781 cgcggacgac ttcgacttcg ggatccgccg ggtcaagatc atccacgcgc cgggccaggc
      841 ccgcggccgg ggcgcgcccc tccagcacgg cggcccactc gtccagcagg acgaccttgc
      901 gcgccgcggg catggcttcg tggtcttcga tgccatcaga gatgcggaac atcaggtagg
      961 ccaccgaaat cgcctcgcgc agcagcggcg gcaactgctc gatggacagg gcgaatgtgc
     1021 ggctgacctg cctgaccatg acatcgcgtt tctgcatacg a
//
LOCUS       DAOSMQ010000701         1059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_142811_length_1059_cov_4.333665, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000701 DAOSMQ010000000
VERSION     DAOSMQ010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142811_length_1059_cov_4.333665"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /gene="nth"
                     /locus_tag="PK829_15500"
     CDS             <1..393
                     /gene="nth"
                     /locus_tag="PK829_15500"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU42672.1"
                     /translation="FYRNKARFIRETAARLLAEYGGVVPPEMDALLTLPGVARKTANV
                     VRGEIYALADGITVDTHVKRLAARLGLTAATDPVKVERDLMAIIPPESWIEIAHLLIW
                     HGRRVCAARKPNCPACPLRDICPSAVGQ"
     gene            444..>1059
                     /locus_tag="PK829_15505"
     CDS             444..>1059
                     /locus_tag="PK829_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42673.1"
                     /translation="MPYFNYDVHAVDVGAASWWERRAFAGAWWALGRQDDRWTPPRYT
                     AFRRDLNPAHNDHLARLAARLVHVEALHRTGVSRSRTDQQEIPLISVLERPLAAAVVT
                     VDPRRRGQTAHLGLLHTAGDAEALERLFDYLTETLAEDGTHRVVGPAGLSPHLSSGAL
                     VDAWDAMPPQHTPTNPPYLPEMLGRDARVLQTGRLYGVEVAGGSV"
BASE COUNT          153 a          390 c          350 g          166 t
ORIGIN      
        1 ttctaccgca ataaggcccg cttcatccgc gagacggccg cgcggctgct ggccgaatat
       61 ggcggcgtcg tgccgccgga gatggacgcc ctgctgacgc tgcccggcgt ggcccgcaag
      121 acagccaacg tcgtccgcgg cgaaatctac gccctggccg atggcatcac cgtcgatacc
      181 cacgttaagc gcctggccgc tcgactggga ctgacggcgg ccaccgaccc ggtcaaggtc
      241 gagcgcgacc tgatggccat catcccgccg gagagctgga ttgagatcgc ccatttgctc
      301 atctggcacg gccgccgcgt ctgcgccgcc cgcaagccca attgcccggc ctgtccgttg
      361 cgggatattt gcccctcggc ggtggggcag tagctcagta ggtaagcagg cggcggtcat
      421 ccttcatcct tcgatgttaa gcgatgccct acttcaacta cgacgttcac gcggttgacg
      481 tcggcgcggc ttcctggtgg gagcggcggg cgtttgccgg cgcgtggtgg gcgctgggac
      541 ggcaggacga ccgctggacg ccgccgcgct acaccgcctt ccggcgcgac ttgaacccgg
      601 ctcacaacga ccacctcgcc cgtctggcgg cccggctcgt ccacgttgag gccctccacc
      661 gcactggcgt ctcccgctcg cgcaccgacc agcaagagat tcccctcatc agcgttctgg
      721 agcggccgct ggccgccgcc gtcgtcaccg tggacccgcg ccgccgcggc cagaccgccc
      781 acctgggcct gctccacacc gccggcgacg ccgaagcgct cgaacggctc ttcgactacc
      841 tgacggagac gctggctgaa gatggcaccc accgcgtcgt cggcccggcc ggcctatcgc
      901 cccacctgag cagtggcgcg ttggtcgacg cctgggacgc catgccgccg caacacacgc
      961 ccaccaatcc gccctacctg ccggagatgc tggggcgcga cgcgcgggtg ttgcagacgg
     1021 ggcggctgta cggtgtggag gtggcgggcg gctctgtgg
//
LOCUS       DAOSMQ010000702         1054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_143917_length_1054_cov_2.842843, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000702 DAOSMQ010000000
VERSION     DAOSMQ010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143917_length_1054_cov_2.842843"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PK829_15510"
     CDS             complement(<1..513)
                     /locus_tag="PK829_15510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU42674.1"
                     /translation="MSLSEAATAESGAWPEANLSGEAQMAHEAALVARARAGDADAWA
                     ALVGHHQEPVFRLAYLILGDAADAEDVAQEAFVRAYLALARFDAGRPLRPWLLSIAAN
                     LARNRRRSLGRYWAAMQRAFLAEPRRHHAPPERTPASDARRLRQAVGRLRPDARDVVY
                     LRYFLGLSEAE"
     gene            543..986
                     /locus_tag="PK829_15515"
     CDS             543..986
                     /locus_tag="PK829_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU42675.1"
                     /translation="MVPMNLEISTDPARLDVAMIHRFLSERSYWAQGRPLDVVRRAID
                     NSLCFGAYLDGQQVAFCRLVTDRATFAWLADVFVLEDVRGQGIGKALVAAALVHPDVQ
                     GARLLLATKDAHGLYAPYGFAPVAPDRYMTRPAKTSSPHGLGDRG"
BASE COUNT          158 a          356 c          378 g          162 t
ORIGIN      
        1 ctccgcctcc gacaggccca ggaagtagcg cagataaaca acgtcgcgag cgtcgggccg
       61 cagccggccg acggcctggc gcaggcggcg ggcgtcgctg gccggcgtgc gctccggcgg
      121 cgcgtggtgg cggcgcggct cggccagaaa ggcgcgctgc atggccgccc aatagcggcc
      181 caggctccgc cgccggttgc gggccaggtt ggcggcgatg ctgagcagcc acgggcgcag
      241 cggccggcca gcatcgaatc gcgccagagc cagataggcg cgcacaaacg cctcctgggc
      301 gacatcctcg gcgtcggcgg cgtcgcccag gatgagatag gccaggcgga agactggctc
      361 ctgatgatgg ccgacaagcg ccgcccaggc gtcagcgtcg ccggcgcgcg cccgcgccac
      421 cagcgccgcc tcgtgggcca tctgggcctc gcctgacaga ttcgcttccg gccacgcgcc
      481 cgattcagcc gtggccgcct cgctcaagct cattcatttc actatacacc gcgtttgcta
      541 gaatggttcc catgaacctg gaaatctcga ccgatcccgc gcggttggac gtggccatga
      601 tccaccgctt tctgtcggag cgctcctact gggcgcaggg gcgtccgctg gacgtggtgc
      661 ggcgggccat cgacaactcg ctctgcttcg gggcctatct cgacgggcag caggtggctt
      721 tctgtcgcct ggtgaccgac cgggcgacat tcgcctggct ggccgatgtg tttgtactgg
      781 aggacgttcg cgggcagggc atcggcaagg cgctggtggc ggcggcgctg gtccatcccg
      841 acgtgcaggg ggcgcggttg ctgctggcga cgaaggacgc ccacggcctc tacgcgccgt
      901 atggctttgc gccggtggcc ccagatcgct acatgacgcg cccggcgaag acgagcagcc
      961 cgcacgggct gggagatagg gggtaggggc taggggctag ggaagagcgc tcttccccta
     1021 gcccctagcc cctaattgtc ggctgcaaag acat
//
LOCUS       DAOSMQ010000703         1048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_145219_length_1048_cov_5.277946, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000703 DAOSMQ010000000
VERSION     DAOSMQ010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145219_length_1048_cov_5.277946"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK829_15520"
     CDS             complement(<1..255)
                     /locus_tag="PK829_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42676.1"
                     /translation="MMRFHVSHPLSNSFRNGLAVAFLLLLGALALRPGPATAAPAVNS
                     IIPAGPTTILNDGGALCALLPSGAVQCWGVEGVQPGPYVAV"
     gene            complement(553..>1048)
                     /locus_tag="PK829_15525"
     CDS             complement(553..>1048)
                     /locus_tag="PK829_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOU42677.1"
                     /translation="LPDGEEARTILDLNARLLAAFGLERGATHAEFIRGADGQYYFLE
                     VAARVGGANIEQLVESASGLNLWGEWARMEIAYAQRQPYAVAPTRHDYAGILVCLARQ
                     EWPDLSGYTDPEVVYRVDKKHHAGLVVASPDAGRVEALLNDYANRFAGDFLAVLPPLD
                     KAPT"
BASE COUNT          202 a          327 c          356 g          163 t
ORIGIN      
        1 gacagcgacg taggggccgg gctggacgcc ctcaacgccc cagcactgca ccgcacccga
       61 cggcagcagg gcgcagagcg cgccgccgtc attgagaatc gtcgtcggcc cggcggggat
      121 gatggaattg accgccgggg cggcggtggc cgggccgggc cgcagcgcca gcgcccccaa
      181 gagcaacaaa aacgcaacgg ccaggccgtt gcggaaagag ttgctgaggg gatgagaaac
      241 atggaatcgc atcactgtgt gtctcctgat aaggtctgag atgttcacga ttacgccgac
      301 aactccggtt agctgtcggg cttcctaccg ttgaaaggcg cgggcgctct gccccgaaaa
      361 ccaagcgcgg cgcacttcac aaagtgcgtc gcgctggcat gacaaggcgt cttccgaatc
      421 catagcacag gtgtagcaaa gatgggatga agctatgctt gatgcgggca aaaatagcac
      481 aagaacgtca ggagtacaat aaaaatcgta gctcgcgttt ctgatggcct tggtggagat
      541 atgagtttgc gcttacgtcg gcgccttatc cagcggcggc agcacggcca gaaagtcccc
      601 ggcaaagcgg ttggcatagt cgttgagcag cgcctccacg cggccggcgt cgggcgaagc
      661 gacgacaagg ccggcgtggt gcttcttgtc cacgcggtag accacttccg ggtcggtgta
      721 gccgctcagg tcgggccact cctgccgcgc caggcagacg aggatgccgg cgtagtcgtg
      781 gcgcgtcggg gcgacggcgt agggctgccg ctgggcatag gcgatctcca tgcgcgccca
      841 ctcgccccac aggttcagcc cgctggccga ctcgacgagc tgctcgatgt tggccccgcc
      901 cacccgcgcg gccacttcga ggaagtaata ctgcccgtcc gcgccgcgga tgaactcggc
      961 atgggtcgcg ccgcgctcca ggccgaaagc ggccagcagg cgggcgttca ggtcgaggat
     1021 ggtccgcgcc tcctcgccat cgggcagc
//
LOCUS       DAOSMQ010000704         1043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_146303_length_1043_cov_3.647773, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000704 DAOSMQ010000000
VERSION     DAOSMQ010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146303_length_1043_cov_3.647773"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PK829_15530"
     CDS             complement(<1..923)
                     /locus_tag="PK829_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOU42678.1"
                     /translation="MRSLTLKLVLAFLTVGLAGVVLVAVVIRRQTLLEFDRFIVDQYQ
                     AEIIARAADYYTENGSLSGVESVLRTGRRGGPGGGPGGARPLLVTDTEGQVVASTGSI
                     ESDGYTSSVNVTDSNAVPVLVNGTAIGYVDFLDMPEADELRATAEAAFLDRISRAILI
                     SAVVAGLLALVLGAALASTIARPVRELTDGTRALAAGDLGHQVPVRTADEIGQLAHSF
                     NQMSADLARSNQLRKQMTADIAHDLRTPLSVILGYSEALQDEKLPGTPEVYGAMYRQA
                     QQLNRLIDDLRILSLADAGQLVHQAMQAAHVL"
     gene            complement(927..>1043)
                     /locus_tag="PK829_15535"
     CDS             complement(927..>1043)
                     /locus_tag="PK829_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU42679.1"
                     /translation="VHIRNLRTKIEPDSRNPRYVLTVYGIGYRLAAEPNGSN"
BASE COUNT          169 a          329 c          369 g          176 t
ORIGIN      
        1 agaacatggg cggcctgcat ggcctgatgg acaagttgcc cggcgtcggc cagcgacagg
       61 atgcgcaggt catcgatgag ccggttcagc tgttgcgcct gacggtacat cgccccgtag
      121 acctccggcg tgccgggcag cttctcgtct tgcagggcct cggagtaacc caggatgacg
      181 ctgaggggcg tgcgcaggtc gtgggcgatg tcggccgtca tctgcttgcg tagctggttg
      241 gagcgggcca ggtcggcgct catctggttg aaggagtggg ccaactggcc gatctcgtcg
      301 gccgtgcgca ccggcacctg gtggccgagg tcgccggcgg ccagcgcccg cgtgccgtcg
      361 gtcaattcgc gcaccggtcg ggcaatggtg ctggccagcg ccgcgcccag aaccagggcc
      421 agcaacccgg cgacaacggc gctgatgaga atggcgcggc tgatgcgatc cagaaaggcg
      481 gcttcggctg tggctcgcag ttcgtcagcc tcgggcatgt ccaggaaatc gacgtagccg
      541 atcgccgtgc cattgaccag cacaggcacg gcattgctgt cggtcacgtt gacggatgat
      601 gtgtagccgt ctgactcgat actgcccgtc gaggcgacga cttgcccctc tgtgtccgtc
      661 accagcaggg ggcgcgcccc gcccggcccg ccgcccggcc cgccgcgccg gccggtgcgc
      721 aagacgctct ccacgcccga taggctgccg ttctccgtgt aatagtcggc ggcgcgggcg
      781 atgatctcgg cctggtactg gtcgacgatg aagcggtcga actccagcag cgtctgccgg
      841 cggatgacca cagcgacgag caccacgccc gccaacccaa ccgtgaggaa tgccaggacg
      901 agtttgaggg tcagggaacg catgatctag ttactgccat tcggctcggc ggccaggcgg
      961 tagccgatgc cgtaaacggt caggacatag cgcgggttgc gcgagtcggg ttcgatcttc
     1021 gtgcgcagat tgcggatgtg gac
//
LOCUS       DAOSMQ010000705         1034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_148388_length_1034_cov_3.419816, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000705 DAOSMQ010000000
VERSION     DAOSMQ010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148388_length_1034_cov_3.419816"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /locus_tag="PK829_15540"
     CDS             complement(<1..>1034)
                     /locus_tag="PK829_15540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOU42680.1"
                     /translation="LTVVVSPLISLMEDQVMQMRELGVPAAYLNSTLAYDSYVTIARQ
                     AATGGIKLLYVAPETLLRPETLTLLDQAGVDCLTIDEAHCISEWGHDFRPEYRQLAAV
                     RRRFPDAVCLAVTATATARVRDDIRASLAIPDAAVFISSFDRANLFLAVEPRVDALGQ
                     ALAFLENHRGQSGIIYCATRRAVDTLTEQLAAHGWNVLPYHAGLDNATRQRNQRRFTY
                     DEAAIVVATVAFGMGIDKSNVRFILHYDLPKDLESYYQQIGRAGRDGLRADCLLLFGA
                     QDISTNIYLIDQGDPAQRPGAIARLQAMLAYAETDQCRRVPMLAYFGETDVPATCSLC
                     DNCTAGAGEQ"
BASE COUNT          170 a          316 c          378 g          170 t
ORIGIN      
        1 gttgttcgcc cgccccggca gtgcagttgt cgcacagact gcacgtcgcc ggcacatccg
       61 tctcgccgaa gtaggccagc atcggcacac gacggcactg gtccgtctcg gcgtaggcca
      121 gcatggcctg tagccgggcg attgcgccgg gacgctgcgc cgggtcgccc tggtcgatga
      181 ggtagatgtt ggtggagatg tcctgcgcgc cgaagagcaa caggcagtcg gcccgcaggc
      241 cgtcgcggcc ggcgcggccg atctgctggt agtagctctc caggtctttg ggcaggtcgt
      301 agtggaggat gaagcgcacg ttggacttgt cgatgcccat gccgaaggcc accgtggcca
      361 caacgatggc cgcttcgtca taggtgaagc gccgctggtt gcgctggcgc gtggcgttgt
      421 ccagcccagc gtggtagggc agcacgttcc agccgtgggc ggccagttgt tcggtcagcg
      481 tgtccacggc gcggcgggtg gcgcagtaaa tgatgcccga ttggccgcgg tggttttcca
      541 gaaaggccag cgcctggccc agggcatcca cgcgcggttc cacagccaga aagaggttgg
      601 cgcggtcgaa gctggaaatg aagacggccg cgtcggggat ggccagcgag gcgcgaatgt
      661 cgtcgcggac gcgcgccgtg gccgtggccg tcaccgccag gcagacggca tcggggaagc
      721 gccggcgcac cgcggccaac tggcgatatt ccggccggaa gtcgtggccc cactcggaga
      781 tgcaatgagc ttcgtcgatg gtcagacagt caacgccggc ctggtcgagc agggtcagcg
      841 tctccggccg cagcagcgtc tccggagcga cgtagagcag cttgatcccg cctgtggctg
      901 cctgccgggc gatggtgaca tagctgtcgt aggccagggt gctgttcaaa taggcggcgg
      961 gcacgcccaa ctcgcgcatc tgcatcacct ggtcttccat cagcgaaatg agcggcgaga
     1021 cgacgacggt cagg
//
LOCUS       DAOSMQ010000706         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_151812_length_1020_cov_2.043523, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000706 DAOSMQ010000000
VERSION     DAOSMQ010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151812_length_1020_cov_2.043523"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK829_15545"
     CDS             complement(<1..206)
                     /locus_tag="PK829_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42681.1"
                     /translation="MTETKPTYAVTEWHGCYDDSWNGVLVPDAFAHPAKFAPGLISRI
                     LRHMLERGYIAPGDSIVDPFGGIAA"
     gene            complement(199..330)
                     /locus_tag="PK829_15550"
     CDS             complement(199..330)
                     /locus_tag="PK829_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42682.1"
                     /translation="MTTLLVAVIVAAVIAAGLLLLVGLCRAAADTRDVAEDTGEYHD"
     gene            complement(327..494)
                     /locus_tag="PK829_15555"
     CDS             complement(327..494)
                     /locus_tag="PK829_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42683.1"
                     /translation="MNEFPTLLQYIGGLTAFLFLSAVLLALYHAGRRAIHGRRPRRYV
                     TAQRAGRGAQR"
     gene            complement(491..814)
                     /locus_tag="PK829_15560"
     CDS             complement(491..814)
                     /locus_tag="PK829_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42684.1"
                     /translation="MTRKTPSRIKPPQVYAGDTAAQLKTTCPTCGVVIMVQAQADKFC
                     DPPDWQFWGYKDARWVRCRGESDGRAYIHFWTIINGNRSDRLAWINPTPDDAPAQKTL
                     FEVNP"
BASE COUNT          158 a          325 c          342 g          195 t
ORIGIN      
        1 gccgcaatgc cgccgaacgg gtcaacaatg ctatcacccg gcgcgatgta gccgcgctcc
       61 agcatgtgcc gcaagatgcg cgagataagg ccgggcgcga atttggccgg gtgcgcgaaa
      121 gcgtccggca ctaggacgcc gttccaactg tcatcatagc agccgtgcca ttctgtcacg
      181 gcgtaggttg gcttcgtctc agtcatggta ctctcctgtg tcctctgcca catcgcgcgt
      241 gtcggccgcg gcccggcaga ggccaacgag cagcagcagg ccggcggcga tgacggccgc
      301 cacgatgacg gcaacgagca gggttgtcat cgttgcgccc ctcggccggc ccgttgcgcc
      361 gtcacgtacc ggcgcggccg gcggccgtgg atggcccgcc gcccggcgtg gtagagcgcc
      421 agcagaacgg cgctcaggaa caggaaggcg gtgaggccgc cgatgtattg gagtagggtg
      481 ggaaattcgt tcatgggttc acctcgaaga gcgtcttttg cgcgggggcg tcatcgggcg
      541 tggggtttat ccacgccagg cggtcgctcc ggttgccgtt gatgattgtc cagaagtgga
      601 tgtaggcgcg gccatcagac tcccctcggc aacgcaccca gcgggcgtct ttgtagcccc
      661 aaaactgcca atcgggcgga tcgcaaaact tgtccgcttg cgcctgaacc atgatgacca
      721 cgccgcacgt cgggcaggtg gttttcagtt gcgcggccgt gtccccggca tacacttgcg
      781 gcggcttgat gcgtgatggt gtttttcgtg tcatagcgtc ttgcccctgg ccggggctga
      841 ggattgcgcc ctcagccccg gcgttgtggc tgtctaaaac gggatgtctt cgctatcggt
      901 cacggccaga tcaggccggg cggtctgggg ctggttggcc gcctcgacag ccgccgcctt
      961 catccactcc gccacccact cgtcgagcat ctcgtcgatg gcgccggttt cctcgccgac
//
LOCUS       DAOSMQ010000707         1015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_153007_length_1015_cov_2.003125, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000707 DAOSMQ010000000
VERSION     DAOSMQ010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153007_length_1015_cov_2.003125"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK829_15565"
     CDS             <1..486
                     /locus_tag="PK829_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42685.1"
                     /translation="AVGNSDAHGTPMHLGPIHREVYPYEFLFRAVNTHVLTPEPLNGD
                     FAHDQALLLRAIGRGHGWVGYDMPASTAGFRYTVTGVRKGIIGDEMPLGATGATLQVK
                     APARCRIRLIRHGVVVAEAQRDTNLAYAPAEPGAYRVECHIPYEGQERGWIYSNPIYL
                     R"
     gene            complement(589..>1015)
                     /locus_tag="PK829_15570"
     CDS             complement(589..>1015)
                     /locus_tag="PK829_15570"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOU42686.1"
                     /translation="LAAMGQAGLTVTPLQAALATATLATGGQRPVPRLVEAVTDASGQ
                     WVSQPSALVGETAVSASTAVAVRETWPLSGDGGELAASSREFAVSTLSGPDGHYNSWY
                     LGFAPADAPRFVVALVLEDEDAITTAEAVGRAVLAAAAR"
BASE COUNT          156 a          368 c          339 g          152 t
ORIGIN      
        1 gccgtgggca acagcgacgc ccacggcacg ccgatgcacc tcggccccat ccaccgcgag
       61 gtctacccct atgagttcct cttccgcgcg gtcaacaccc acgtgctgac accggagccg
      121 ctcaacggcg attttgccca tgaccaggcg ctgttgctgc gagccatcgg ccgcggccac
      181 ggttgggtcg gctacgacat gcccgcttcc acggctggct ttcgctacac ggtcacgggt
      241 gtccgcaagg gtattatcgg cgacgagatg ccgctgggcg cgacgggggc gacgctacag
      301 gtgaaggcgc cggcgcgctg ccgcatccgg ctcatccgcc acggcgtcgt cgtggccgaa
      361 gcccagcgcg acaccaacct ggcctacgcg ccggccgagc cgggggccta ccgtgtggag
      421 tgccacatcc cgtatgaggg gcaggagcgg ggctggatct acagcaatcc catctacttg
      481 cgctgacttt atggccgtgc ctggcacttt ccgaagcgcc gggcacgttc ttgagcggat
      541 acccgaagga ggtgcgacgc actttctgaa gtgcgacgcg ccgagcggtt accttgccgc
      601 cgccgcgagg acggcccggc cgaccgcctc ggccgtggtg atggcgtctt catcttccag
      661 caccagagcg acgacgaagc gcggcgcgtc ggccggggca aaacccagat accagctatt
      721 gtagtggccg tccgggccgg agagggtcga cacggcgaac tcccgcgaac tcgccgcgag
      781 ttcgcccccg tcgccgctaa gtggccacgt ctcgcgcaca gcgacggccg tggacgcgct
      841 gacagccgtc tcgccgacga gggccgacgg ctggctgacc cattggccgc tggcatccgt
      901 gacggcctcc accagccgcg gcacgggccg ttggccgccg gtagccagcg tcgccgtggc
      961 cagcgccgct tgcagcggcg tcactgtcag ccccgcctga cccatcgctg ccagg
//
LOCUS       DAOSMQ010000708         1014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_153155_length_1014_cov_2.982273, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000708 DAOSMQ010000000
VERSION     DAOSMQ010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153155_length_1014_cov_2.982273"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..668
                     /gene="pth"
                     /locus_tag="PK829_15575"
     CDS             57..668
                     /gene="pth"
                     /locus_tag="PK829_15575"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU42687.1"
                     /translation="MDSAERGKRFLIVGLGNPGRNHAFNRHNVGFMAIDRLALAQGID
                     MRRVQSKAIVGSGRLAEGAVVLAKPQTFMNLSGEAVGALAGFYRIPPSAVLVVYDELD
                     IPFGVIRLREKGGAGGHNGMRSIIQHLGNDFPRLRLGIGRPPGRMDPAAYVLQDFGRD
                     ELPLVSEMLATAGQAITSFVRDGIDLTMSRFNGPVNGPAEEQG"
     gene            665..>1014
                     /locus_tag="PK829_15580"
     CDS             665..>1014
                     /locus_tag="PK829_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU42688.1"
                     /translation="MNVSGLLDLFDQIPAFAQLVGALGDAEGQPLLPLDLPRGARAPV
                     VAQIHRQLSAPARAPILLLCGRVDTATLWHQALETWLPASSRLHRLPEPTPLPYDRGP
                     WSDRTRAGRLTALT"
BASE COUNT          157 a          359 c          337 g          161 t
ORIGIN      
        1 ccgtcggagt tgtggaacgc ctatgaattg gacatgacgg tgggaggctc gtagacgtgg
       61 atagcgctga gcgcggcaaa cggtttctga tcgtcgggct gggcaacccc ggccggaacc
      121 acgccttcaa ccgccacaac gtcggcttca tggccatcga ccggctggct ctggcccagg
      181 gcatcgacat gcgccgcgtc caaagtaaag ccatcgtcgg cagcggccgg ctggccgagg
      241 gcgcggtcgt tctggccaag ccgcagacct tcatgaacct tagcggcgag gccgtcggtg
      301 cgctggccgg cttctaccgc attccgccca gcgccgtgct ggtggtctac gacgagttag
      361 acatcccctt cggtgtcatc cggctgcgcg agaagggcgg cgcgggcggc cacaacggca
      421 tgcgctccat catccagcac ctgggcaacg acttcccgcg gctgcggctg ggcattggcc
      481 ggccgccggg gcgcatggac ccggccgcct acgtcctgca agacttcggt cgcgacgaac
      541 tgccgctggt cagtgagatg ctggccacag ccggccaggc catcacgtcg ttcgtccgcg
      601 acggcattga cctgactatg tcgcgcttca atgggccggt gaacggccct gcagaggaac
      661 agggatgaac gtatccggac tgctcgacct gttcgaccag attcccgcct ttgcccaact
      721 tgtcggggca ctgggcgacg ccgaaggcca gccgctgctg ccgctggacc tgccgcgcgg
      781 ggcgcgcgcg cctgtcgtcg cccagatcca ccggcagttg agcgcgccgg cccgcgcgcc
      841 cattctgctg ctctgcggcc gagtcgatac ggccacgctg tggcatcagg ccctggagac
      901 atggctgccc gccagcagcc gccttcatcg cctgccggag ccaacgcctc tgccctacga
      961 ccgcggcccg tggagcgacc ggacgcgcgc cggccgcctg acagccctga ccga
//
LOCUS       DAOSMQ010000709         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_2393
            NODE_155651_length_1004_cov_5.050580, whole genome shotgun
            sequence.
ACCESSION   DAOSMQ010000709 DAOSMQ010000000
VERSION     DAOSMQ010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864460
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:38:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,117
            CDSs (total)                      :: 3,092
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 5 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155651_length_1004_cov_5.050580"
                     /isolate="AMR_MDS_2393"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PK829_15585"
     CDS             complement(<1..902)
                     /locus_tag="PK829_15585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU42689.1"
                     /translation="MTLKTGDVLQNRYRIVRLVGQGGFGAVYRAWDINVSQPVALKEN
                     LGVGSEAQRQFEREATLLAGLRHPNLPRVTDHFIIPGQGQYLVMDYVEGKSLATLLAE
                     RGRPLPEADALAWVRQVCDALTYLHARPSPIIHRDIKPENIIVTADNRAMLVDFGISK
                     VFVAGGQTTVGAMAVTPGFSPPEQYGGGGTDARSDVYALAATLYKLLTAQTPPESVEV
                     MAAEVVVPPAHELNPAVSPAVARAIERAMTPQMSRRTANATTFAEELLSRLAMPLPTA
                     AILAPIAVAERAASPARPEPAPASA"
BASE COUNT          155 a          327 c          361 g          161 t
ORIGIN      
        1 gcgctggccg gggctggctc cggccgcgcc gggctggcgg cgcgctccgc gacggcgatg
       61 ggcgcgagga tagctgctgt gggcagcggc atggcgaggc gcgacagcaa ctcctcggcg
      121 aacgtggtcg cgttggccgt gcgccggctc atctgtggcg tcatagcccg ctcgatggcc
      181 cgcgccacgg cgggactcac ggccgggttc agttcgtgcg ccggcggcac aaccacctcg
      241 gccgccatca cctcgacgct ttccggtggc gtctgcgcgg tcagcagctt gtagagcgtc
      301 gccgccaggg cgtagacatc gctgcgggcg tccgtgccgc cgccaccata ctgctccggt
      361 gggctgaagc cgggcgtgac ggccatcgcg ccgacggtcg tctgaccgcc ggccacgaag
      421 accttggaga tgccgaaatc gaccagcatg gcccggttgt cggccgtgac gatgatattc
      481 tccggcttaa tgtcgcggtg gataatgggc gaggggcgcg cgtgcaggta agtcagcgcg
      541 tcgcagacct gccgcaccca ggccagggcg tcggcctccg gcagcggccg tcctcgctcg
      601 gccagcagcg tcgctaggct cttgccctcg acgtagtcca tgaccaggta ttgcccctgg
      661 ccgggaatga tgaagtggtc ggtgacgcgg gggaggttgg ggtggcgtag gccggccagc
      721 agtgtcgcct cgcgttcgaa ctggcgctga gcctcgctgc cgacacccag gttttccttc
      781 agcgctaccg gctgggagac attgatatcc caggcgcgat agaccgcgcc gaagccaccc
      841 tggccaacca ggcggacgat gcgatagcgg ttctgcaaaa catcaccggt cttcagcgtc
      901 acggcacgct cctatacttc gcgcggagtg gcgacagggt cgaaacacgg acgctatatt
      961 agcgcatgtg tgacccggcg aaaagggcga gcagaggcgg ccgg
//
